LOCUS       DAZFRN010000001        86139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFRN010000001 DAZFRN010000000
VERSION     DAZFRN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.001"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            14..1216
                     /gene="proV"
                     /locus_tag="ACHKUB_000001"
     CDS             14..1216
                     /gene="proV"
                     /locus_tag="ACHKUB_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO6653091.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1209..2273
                     /gene="proW"
                     /locus_tag="ACHKUB_000002"
     CDS             1209..2273
                     /gene="proW"
                     /locus_tag="ACHKUB_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO6653092.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2331..3323
                     /gene="proX"
                     /locus_tag="ACHKUB_000003"
     CDS             2331..3323
                     /gene="proX"
                     /locus_tag="ACHKUB_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO6653093.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3615..4799
                     /locus_tag="ACHKUB_000004"
     CDS             3615..4799
                     /locus_tag="ACHKUB_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6653094.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4923..5660
                     /gene="ygaZ"
                     /locus_tag="ACHKUB_000005"
     CDS             4923..5660
                     /gene="ygaZ"
                     /locus_tag="ACHKUB_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO6653095.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5650..5985
                     /gene="ygaH"
                     /locus_tag="ACHKUB_000006"
     CDS             5650..5985
                     /gene="ygaH"
                     /locus_tag="ACHKUB_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO6653096.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6076..6606
                     /gene="emrR"
                     /locus_tag="ACHKUB_000007"
     CDS             6076..6606
                     /gene="emrR"
                     /locus_tag="ACHKUB_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO6653097.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6733..7905
                     /gene="emrA"
                     /locus_tag="ACHKUB_000008"
     CDS             6733..7905
                     /gene="emrA"
                     /locus_tag="ACHKUB_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO6653098.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7922..9460
                     /gene="emrB"
                     /locus_tag="ACHKUB_000009"
     CDS             7922..9460
                     /gene="emrB"
                     /locus_tag="ACHKUB_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO6653099.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9524..10039)
                     /gene="luxS"
                     /locus_tag="ACHKUB_000010"
     CDS             complement(9524..10039)
                     /gene="luxS"
                     /locus_tag="ACHKUB_000010"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO6653100.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(10189..11745)
                     /gene="gshA"
                     /locus_tag="ACHKUB_000011"
     CDS             complement(10189..11745)
                     /gene="gshA"
                     /locus_tag="ACHKUB_000011"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO6653101.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(11818..12246)
                     /gene="yqaA"
                     /locus_tag="ACHKUB_000012"
     CDS             complement(11818..12246)
                     /gene="yqaA"
                     /locus_tag="ACHKUB_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO6653102.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12243..12809)
                     /gene="yqaB"
                     /locus_tag="ACHKUB_000013"
     CDS             complement(12243..12809)
                     /gene="yqaB"
                     /locus_tag="ACHKUB_000013"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO6653103.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13090..13166)
                     /locus_tag="ACHKUB_000014"
     tRNA            complement(13090..13166)
                     /locus_tag="ACHKUB_000014"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13130..13132),aa:Arg,seq:acg)
     gene            complement(13365..13441)
                     /locus_tag="ACHKUB_000015"
     tRNA            complement(13365..13441)
                     /locus_tag="ACHKUB_000015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13405..13407),aa:Arg,seq:acg)
     gene            complement(13505..13581)
                     /locus_tag="ACHKUB_000016"
     tRNA            complement(13505..13581)
                     /locus_tag="ACHKUB_000016"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13545..13547),aa:Arg,seq:acg)
     gene            complement(13779..13855)
                     /locus_tag="ACHKUB_000017"
     tRNA            complement(13779..13855)
                     /locus_tag="ACHKUB_000017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13819..13821),aa:Arg,seq:acg)
     gene            complement(13859..13951)
                     /locus_tag="ACHKUB_000018"
     tRNA            complement(13859..13951)
                     /locus_tag="ACHKUB_000018"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13915..13917),aa:Ser,seq:gct)
     gene            complement(14267..14452)
                     /gene="csrA"
                     /locus_tag="ACHKUB_000019"
     CDS             complement(14267..14452)
                     /gene="csrA"
                     /locus_tag="ACHKUB_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO6653104.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14687..17317)
                     /gene="alaS"
                     /locus_tag="ACHKUB_000020"
     CDS             complement(14687..17317)
                     /gene="alaS"
                     /locus_tag="ACHKUB_000020"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO6653105.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17445..17945)
                     /gene="recX"
                     /locus_tag="ACHKUB_000021"
     CDS             complement(17445..17945)
                     /gene="recX"
                     /locus_tag="ACHKUB_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO6653106.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18188..19249)
                     /gene="recA"
                     /locus_tag="ACHKUB_000022"
     CDS             complement(18188..19249)
                     /gene="recA"
                     /locus_tag="ACHKUB_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO6653107.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19329..19826)
                     /gene="pncC"
                     /locus_tag="ACHKUB_000023"
     CDS             complement(19329..19826)
                     /gene="pncC"
                     /locus_tag="ACHKUB_000023"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO6653108.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19971..21056)
                     /gene="mltB"
                     /locus_tag="ACHKUB_000024"
     CDS             complement(19971..21056)
                     /gene="mltB"
                     /locus_tag="ACHKUB_000024"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO6653109.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21312..21875
                     /gene="srlA"
                     /locus_tag="ACHKUB_000025"
                     /gene_synonym="gutA"
     CDS             21312..21875
                     /gene="srlA"
                     /locus_tag="ACHKUB_000025"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO6653110.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21872..22831
                     /gene="srlE"
                     /locus_tag="ACHKUB_000026"
                     /gene_synonym="gutE"
     CDS             21872..22831
                     /gene="srlE"
                     /locus_tag="ACHKUB_000026"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO6653111.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22842..23213
                     /gene="srlB"
                     /locus_tag="ACHKUB_000027"
                     /gene_synonym="gutB"
     CDS             22842..23213
                     /gene="srlB"
                     /locus_tag="ACHKUB_000027"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO6653112.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23217..23996
                     /gene="srlD"
                     /locus_tag="ACHKUB_000028"
     CDS             23217..23996
                     /gene="srlD"
                     /locus_tag="ACHKUB_000028"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO6653113.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24101..24460
                     /gene="gutM"
                     /locus_tag="ACHKUB_000029"
     CDS             24101..24460
                     /gene="gutM"
                     /locus_tag="ACHKUB_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO6653114.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            24527..25300
                     /gene="srlR"
                     /locus_tag="ACHKUB_000030"
                     /gene_synonym="gutR"
     CDS             24527..25300
                     /gene="srlR"
                     /locus_tag="ACHKUB_000030"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO6653115.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25293..26258
                     /gene="gutQ"
                     /locus_tag="ACHKUB_000031"
     CDS             25293..26258
                     /gene="gutQ"
                     /locus_tag="ACHKUB_000031"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO6653116.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26255..27769)
                     /gene="norR"
                     /locus_tag="ACHKUB_000032"
     CDS             complement(26255..27769)
                     /gene="norR"
                     /locus_tag="ACHKUB_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO6653117.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            27956..29395
                     /gene="norV"
                     /locus_tag="ACHKUB_000033"
     CDS             27956..29395
                     /gene="norV"
                     /locus_tag="ACHKUB_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO6653118.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29392..30525
                     /gene="norW"
                     /locus_tag="ACHKUB_000034"
     CDS             29392..30525
                     /gene="norW"
                     /locus_tag="ACHKUB_000034"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO6653119.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKIDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(30653..32905)
                     /gene="hypF"
                     /locus_tag="ACHKUB_000035"
     CDS             complement(30653..32905)
                     /gene="hypF"
                     /locus_tag="ACHKUB_000035"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO6653120.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGAMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33058..33585)
                     /gene="hydN"
                     /locus_tag="ACHKUB_000036"
     CDS             complement(33058..33585)
                     /gene="hydN"
                     /locus_tag="ACHKUB_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO6653121.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33734..34744)
                     /gene="ascG"
                     /locus_tag="ACHKUB_000037"
     CDS             complement(33734..34744)
                     /gene="ascG"
                     /locus_tag="ACHKUB_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO6653122.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            35004..36461
                     /gene="ascF"
                     /locus_tag="ACHKUB_000038"
     CDS             35004..36461
                     /gene="ascF"
                     /locus_tag="ACHKUB_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO6653123.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            36470..37894
                     /gene="ascB"
                     /locus_tag="ACHKUB_000039"
     CDS             36470..37894
                     /gene="ascB"
                     /locus_tag="ACHKUB_000039"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO6653124.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSLSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38008..38427)
                     /locus_tag="ACHKUB_000040"
     CDS             complement(38008..38427)
                     /locus_tag="ACHKUB_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6653125.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(38498..39253)
                     /locus_tag="ACHKUB_000041"
     CDS             complement(38498..39253)
                     /locus_tag="ACHKUB_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653126.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRVD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            complement(39296..39766)
                     /gene="hycI"
                     /locus_tag="ACHKUB_000042"
     CDS             complement(39296..39766)
                     /gene="hycI"
                     /locus_tag="ACHKUB_000042"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO6653127.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            complement(39759..40169)
                     /gene="hycH"
                     /locus_tag="ACHKUB_000043"
     CDS             complement(39759..40169)
                     /gene="hycH"
                     /locus_tag="ACHKUB_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO6653128.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(40166..40933)
                     /gene="hycG"
                     /locus_tag="ACHKUB_000044"
     CDS             complement(40166..40933)
                     /gene="hycG"
                     /locus_tag="ACHKUB_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO6653129.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(40933..41475)
                     /gene="hycF"
                     /locus_tag="ACHKUB_000045"
     CDS             complement(40933..41475)
                     /gene="hycF"
                     /locus_tag="ACHKUB_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO6653130.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(41485..43194)
                     /gene="hycE"
                     /locus_tag="ACHKUB_000046"
     CDS             complement(41485..43194)
                     /gene="hycE"
                     /locus_tag="ACHKUB_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO6653131.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43212..44135)
                     /gene="hycD"
                     /locus_tag="ACHKUB_000047"
     CDS             complement(43212..44135)
                     /gene="hycD"
                     /locus_tag="ACHKUB_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO6653132.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44138..45964)
                     /gene="hycC"
                     /locus_tag="ACHKUB_000048"
     CDS             complement(44138..45964)
                     /gene="hycC"
                     /locus_tag="ACHKUB_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO6653133.1"
                     /translation="MSAISLINSGVAWFIAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(45961..46572)
                     /gene="hycB"
                     /locus_tag="ACHKUB_000049"
     CDS             complement(45961..46572)
                     /gene="hycB"
                     /locus_tag="ACHKUB_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO6653134.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(46697..47158)
                     /gene="hycA"
                     /locus_tag="ACHKUB_000050"
     CDS             complement(46697..47158)
                     /gene="hycA"
                     /locus_tag="ACHKUB_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO6653135.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            47370..47720
                     /gene="hypA"
                     /locus_tag="ACHKUB_000051"
     CDS             47370..47720
                     /gene="hypA"
                     /locus_tag="ACHKUB_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6653136.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            47724..48596
                     /gene="hypB"
                     /locus_tag="ACHKUB_000052"
     CDS             47724..48596
                     /gene="hypB"
                     /locus_tag="ACHKUB_000052"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO6653137.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            48587..48859
                     /gene="hypC"
                     /locus_tag="ACHKUB_000053"
     CDS             48587..48859
                     /gene="hypC"
                     /locus_tag="ACHKUB_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO6653138.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            48859..49980
                     /gene="hypD"
                     /locus_tag="ACHKUB_000054"
     CDS             48859..49980
                     /gene="hypD"
                     /locus_tag="ACHKUB_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO6653139.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            49977..50987
                     /gene="hypE"
                     /locus_tag="ACHKUB_000055"
     CDS             49977..50987
                     /gene="hypE"
                     /locus_tag="ACHKUB_000055"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO6653140.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLELA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            51061..53139
                     /gene="flhA"
                     /locus_tag="ACHKUB_000056"
     CDS             51061..53139
                     /gene="flhA"
                     /locus_tag="ACHKUB_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO6653141.1"
                     /translation="MSYTPMSDLGQQELFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSHPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(53176..53529)
                     /gene="ygbA"
                     /locus_tag="ACHKUB_000057"
     CDS             complement(53176..53529)
                     /gene="ygbA"
                     /locus_tag="ACHKUB_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO6653142.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(53561..53740)
                     /locus_tag="ACHKUB_000058"
     CDS             complement(53561..53740)
                     /locus_tag="ACHKUB_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653143.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            53816..56377
                     /gene="mutS"
                     /locus_tag="ACHKUB_000059"
     CDS             53816..56377
                     /gene="mutS"
                     /locus_tag="ACHKUB_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO6653144.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDCKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            56483..57139
                     /gene="pphB"
                     /locus_tag="ACHKUB_000060"
     CDS             56483..57139
                     /gene="pphB"
                     /locus_tag="ACHKUB_000060"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6653145.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESKLL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(57190..57957)
                     /gene="ygbI"
                     /locus_tag="ACHKUB_000061"
     CDS             complement(57190..57957)
                     /gene="ygbI"
                     /locus_tag="ACHKUB_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO6653146.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            58153..59061
                     /gene="ltnD"
                     /locus_tag="ACHKUB_000062"
     CDS             58153..59061
                     /gene="ltnD"
                     /locus_tag="ACHKUB_000062"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO6653147.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            59058..60320
                     /gene="otnK"
                     /locus_tag="ACHKUB_000063"
     CDS             59058..60320
                     /gene="otnK"
                     /locus_tag="ACHKUB_000063"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO6653148.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWTQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLATYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVARFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            60317..60955
                     /gene="ygbL"
                     /locus_tag="ACHKUB_000064"
     CDS             60317..60955
                     /gene="ygbL"
                     /locus_tag="ACHKUB_000064"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO6653149.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            60960..61736
                     /gene="ygbM"
                     /locus_tag="ACHKUB_000065"
     CDS             60960..61736
                     /gene="ygbM"
                     /locus_tag="ACHKUB_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO6653150.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            61825..63189
                     /gene="ygbN"
                     /locus_tag="ACHKUB_000066"
     CDS             61825..63189
                     /gene="ygbN"
                     /locus_tag="ACHKUB_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO6653151.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(63228..63464)
                     /locus_tag="ACHKUB_000067"
     CDS             complement(63228..63464)
                     /locus_tag="ACHKUB_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO6653152.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(63475..64902)
                     /locus_tag="ACHKUB_000068"
     CDS             complement(63475..64902)
                     /locus_tag="ACHKUB_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO6653153.1"
                     /translation="MAFDDLRSFLQELDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGAFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPAICVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(64902..65495)
                     /locus_tag="ACHKUB_000069"
     CDS             complement(64902..65495)
                     /locus_tag="ACHKUB_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO6653154.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            65642..66049
                     /locus_tag="ACHKUB_000070"
     CDS             65642..66049
                     /locus_tag="ACHKUB_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO6653155.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDSADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(66170..67162)
                     /gene="rpoS"
                     /locus_tag="ACHKUB_000071"
     CDS             complement(66170..67162)
                     /gene="rpoS"
                     /locus_tag="ACHKUB_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO6653156.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(67225..68319)
                     /gene="nlpD"
                     /locus_tag="ACHKUB_000072"
     CDS             complement(67225..68319)
                     /gene="nlpD"
                     /locus_tag="ACHKUB_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO6653157.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(68504..69130)
                     /gene="pcm"
                     /locus_tag="ACHKUB_000073"
     CDS             complement(68504..69130)
                     /gene="pcm"
                     /locus_tag="ACHKUB_000073"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO6653158.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(69124..69885)
                     /gene="surE"
                     /locus_tag="ACHKUB_000074"
     CDS             complement(69124..69885)
                     /gene="surE"
                     /locus_tag="ACHKUB_000074"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO6653159.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(69866..70915)
                     /gene="truD"
                     /locus_tag="ACHKUB_000075"
     CDS             complement(69866..70915)
                     /gene="truD"
                     /locus_tag="ACHKUB_000075"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO6653160.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(70912..71391)
                     /gene="ispF"
                     /locus_tag="ACHKUB_000076"
     CDS             complement(70912..71391)
                     /gene="ispF"
                     /locus_tag="ACHKUB_000076"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO6653161.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVTLHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(71391..72101)
                     /gene="ispD"
                     /locus_tag="ACHKUB_000077"
     CDS             complement(71391..72101)
                     /gene="ispD"
                     /locus_tag="ACHKUB_000077"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO6653162.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(72120..72431)
                     /gene="ftsB"
                     /locus_tag="ACHKUB_000078"
     CDS             complement(72120..72431)
                     /gene="ftsB"
                     /locus_tag="ACHKUB_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO6653163.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(72625..72948)
                     /gene="ygbE"
                     /locus_tag="ACHKUB_000079"
     CDS             complement(72625..72948)
                     /gene="ygbE"
                     /locus_tag="ACHKUB_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO6653164.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(72998..73603)
                     /gene="cysC"
                     /locus_tag="ACHKUB_000080"
     CDS             complement(72998..73603)
                     /gene="cysC"
                     /locus_tag="ACHKUB_000080"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO6653165.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(73603..75030)
                     /gene="cysN"
                     /locus_tag="ACHKUB_000081"
     CDS             complement(73603..75030)
                     /gene="cysN"
                     /locus_tag="ACHKUB_000081"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO6653166.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARIDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(75032..75940)
                     /gene="cysD"
                     /locus_tag="ACHKUB_000082"
     CDS             complement(75032..75940)
                     /gene="cysD"
                     /locus_tag="ACHKUB_000082"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO6653167.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNLEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            76192..77229
                     /gene="iap"
                     /locus_tag="ACHKUB_000083"
     CDS             76192..77229
                     /gene="iap"
                     /locus_tag="ACHKUB_000083"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO6653168.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   77250..77583
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=77372..77400
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(77680..77973)
                     /gene="cas2e"
                     /locus_tag="ACHKUB_000084"
     CDS             complement(77680..77973)
                     /gene="cas2e"
                     /locus_tag="ACHKUB_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO6653169.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(77970..78893)
                     /gene="cas1e"
                     /locus_tag="ACHKUB_000085"
     CDS             complement(77970..78893)
                     /gene="cas1e"
                     /locus_tag="ACHKUB_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO6653170.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(78890..79540)
                     /gene="cas6e"
                     /locus_tag="ACHKUB_000086"
     CDS             complement(78890..79540)
                     /gene="cas6e"
                     /locus_tag="ACHKUB_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO6653171.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRPFVSELRTGQSLCFNLQANPTICKA
                     GKRHDLLMEAKRQVKGQAEGRDIWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENTRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(79522..80268)
                     /gene="cas5e"
                     /locus_tag="ACHKUB_000087"
     CDS             complement(79522..80268)
                     /gene="cas5e"
                     /locus_tag="ACHKUB_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO6653172.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPE
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDTLRNAYQQYQDHFRKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(80279..81334)
                     /gene="cas7e"
                     /locus_tag="ACHKUB_000088"
     CDS             complement(80279..81334)
                     /gene="cas7e"
                     /locus_tag="ACHKUB_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO6653173.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTDYASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(81346..81882)
                     /gene="casB"
                     /locus_tag="ACHKUB_000089"
                     /gene_synonym="cse2"
     CDS             complement(81346..81882)
                     /gene="casB"
                     /locus_tag="ACHKUB_000089"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO6653174.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(81879..83441)
                     /gene="casA"
                     /locus_tag="ACHKUB_000090"
                     /gene_synonym="cse1"
     CDS             complement(81879..83441)
                     /gene="casA"
                     /locus_tag="ACHKUB_000090"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO6653175.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(83539..>86139)
                     /gene="cas3"
                     /locus_tag="ACHKUB_000091"
     CDS             complement(83539..>86139)
                     /gene="cas3"
                     /locus_tag="ACHKUB_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO6653176.1"
                     /translation="DYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQ
                     FFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISHSTLGYWLWNHYLSECQ
                     ELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLEIKP
                     LFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQ
                     KTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILE
                     DVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRP
                     SLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGVG
                     TLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVI
                     LLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDV
                     ERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLS
                     LFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDLAP
                     VDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNS
                     AFVYPDHGRIWLTQRVLREQGAIQMPHSARLLIESVYGEDVVMPEGFARSEQEQVGKY
                     YCDRARAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGA
                     HAWEMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCIEQRQDPEMANVILVTDDESCGYS
                     AMEGLTGKVG"
BASE COUNT        20206 a        23463 c        22138 g        20332 t
ORIGIN      
        1 atctttctat tgcatggcaa ttaaattaga aattaaaaat ctttataaaa tatttggcga
       61 gcatccacag cgagcgttca aatatatcga acaaggactt tcgaaagaac aaattctgga
      121 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
      181 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
      241 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
      301 cgatgccgaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
      361 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
      421 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
      481 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
      541 ccgcgcgtta gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga
      601 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
      661 caccattgtc tttatttccc acgatctcga tgaggctatg cgtattggcg accgaattgc
      721 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
      781 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tattcagtgc
      841 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaacccctg gcttcggccc
      901 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
      961 cggtaataag tttgtcggcg cagtttccat cgattcgctt aaaaccgcgt taacgcagca
     1021 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     1081 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     1141 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     1201 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     1261 tgcaatccgc agacgcctgg ggtacaccgg cgactgcacc gactgacggc ggtggtgctg
     1321 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     1381 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     1441 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttacattctc aacggtttcc
     1501 agcaattgct gctgggtatg cccgcaccgg tggcgattat cgttttcgct ctcatcgcct
     1561 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg
     1621 caatcggtgc ctggtcgcag gccatggtta ccctggcgct ggtgttaacc gccctgctgt
     1681 tctgtatcgt cattggtttg ccgctgggga tctggctggc gagaagtccg cgagcggcga
     1741 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc
     1801 caatcgtcat gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg
     1861 ctctgccgcc gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga
     1921 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     1981 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta
     2041 tggtggtcat tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     2101 tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     2161 ttatcctcga ccgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     2221 gctggtacac cactggccct gttggtctgc tgacccgccc attcattaag taactctgca
     2281 cttgcccggt gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     2341 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgacc
     2401 tgccgggcaa aggcattaca gttaatccag ttcaaagcac catcaccgaa gaaaccttcc
     2461 agacactgct ggtcagtcgt gcgctggaga aattaggtta taccgtcaat aaacccagcg
     2521 aagtagatta caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg
     2581 tgaactggac gccgctgcat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     2641 atcgtgaagg ggtatttgtt aacggcgcgg cacagggtta cctgatcgat aagaaaaccg
     2701 ccgaccagta taaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     2761 tcgataccaa cggcgacggt aaagcggatt taaccggttg taaccctggc tggggctgcg
     2821 aaggtgccat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     2881 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     2941 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     3001 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaatgcc gataccaaac
     3061 tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     3121 cctggaccga gaaaaatccg gcggcagcca aactgtttgc cattatgcag ttgccagtgg
     3181 ccgatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     3241 agggccacgt tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     3301 aggcgctggc agcgcagaag taatttttat tcaggcggat aaggcgtttt tgcctcatcc
     3361 gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc ttatcatgcc tacaaaatac
     3421 actcattccg tatggcggat aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc
     3481 tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaccac atcgcacatt tcaacaagct
     3541 atctttcatc ccatattcat caacatccgc tattattgat ttccagctta atcatcacct
     3601 gatgaacaaa aataatgact aaacctaatc atgagcttag cccggcgctg atcgtgctga
     3661 tgtctatcgc caccggtctg gcggtcgcca gtaactatta cgctcagcca ttgctcgaca
     3721 ccatcgcgcg taacttttcc ctttccgcca gttcggcagg ctttattgtt accgccgcgc
     3781 aattgggcta tgcagcaggt ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc
     3841 gccgcctgat tgtctcgatg accttactgg cggcaggcgg catgttaatc actgccagca
     3901 gtcagtcgct ggcgatgatg atcctcggta cggcattaac cggtttattc tctgtcgtgg
     3961 cacaaattct ggttccgctg gcagcgacgc tggcttcacc ggacaaacgc ggcaaagtgg
     4021 ttggcactat tatgagcggt ctgctgttgg ggatcttgct ggcacggaca gttgccggat
     4081 tgctggcgaa tctcggcggc tggcgcaccg tcttttgggt tgcctcggtg ttaatggcac
     4141 tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa atcagaaacc cacctcaact
     4201 acccacagtt gttaggttcc gttttcagca tgtttatcag cgataagatc ctgcgcaccc
     4261 gcgcattgct gggctgcctg acctttgcca acttcagcat tctctggacc tcaatggcct
     4321 ttttgcttgc cgctccacct tttaactaca gcgatggtgt cattggtctg tttggacttg
     4381 cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg ttttgccgat aagggtaaat
     4441 cgcaccacac cacaactttc ggtctgctgc tgctattact ttcatggctg gcgatctggt
     4501 ttggtcacac ttccgtactg gcgctgatta tcggcatcct ggtgctggat ctcaccgtac
     4561 agggcgtgca tatcactaac cagacggtaa tttatcgaat acaccctgat gcgcgtaatc
     4621 gcctgaccgc aggttacatg accagctact ttattggcgg tgccgccggt tcgctaattt
     4681 cagcctcagc ctggcaacat ggcggttggg ctggcgtttg tctggctggc gcgacgattg
     4741 ccctggttaa cttactggtc tggtggcgag gttttcatcg tcaggaagcc gcaaattaag
     4801 caatcagcaa ccgttttcgt ggctttacac ttataagggt gttaagaagc ccatcagtct
     4861 gataaggtta agatattcat tcagtctatt tataatatta acaatcgtta agcgtacact
     4921 ctatggaaag ccctactcca cagcctgctc ctggttcggc gaccttcatg gaaggatgca
     4981 aagacagttt accgattgtt attagttata ttccggtggc ctttgcgttc ggtctgaatg
     5041 cgacccgtct gggattctct cctctcgaaa gcgttttttt ctcctgcatc atttatgcag
     5101 gcgcgagcca gttcgtcatt accgcgatgc tggcagccgg gagtagtttg tgggttgctg
     5161 cactgaccgt catggcaatg gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc
     5221 gtattattca gcgtctgcaa aaatcgaaaa ccgccctgtg ggcatttggc ctgacggatg
     5281 aggtttttgc cgccgcaacc gcaaaactgg tacgcaataa tcgccgctgg agcgagaact
     5341 ggatgatcgg cattgccttc agttcatggt catcgtgggt atttggtacg gtaatagggg
     5401 cattctccgg cagcggcttg ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta
     5461 tgcttccggc actctttatg agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt
     5521 gcgttaccgc agcgttagtt ggtgcccttg caggcgtaac gctattttct attcccgtcg
     5581 ccattctggc aggcattgtc tgtggctgcc tcactgcgtt aatccaggca ttctggcaag
     5641 gagcgcccga tgagctatga ggttctgctg cttgggttac tggttggcgt ggcgaattat
     5701 tgcttccgct atttgccgct gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc
     5761 gcggtaggta ttttgctcga caccattggc atcgcctcga tatgcgctct gctggttgtc
     5821 tctaccgcac cagaagtgat gcacgataca cgccgtttcg tgcccacgct ggtcggcttc
     5881 gcggtactgg gtgccagttt ctataaaaca cgcagcatta tcatcccaac actgcttagt
     5941 gcgctggcct atgggctcgc ctggaaagtg atggcgatta tataacccac aagaatcatt
     6001 tttctaaaac aatacattta ctttatttgt cactgtcgtt actatatcgg ctgaaattaa
     6061 tgaggtcata cccaaatgga tagttcgttt acgcccattg aacaaatgct aaaatttcgc
     6121 gccagccgcc acgaagattt tccttatcag gagatccttc tgactcgtct ttgcatgcac
     6181 atgcaaagca agctgctgga gaaccgcaat aaaatgctga aggctcaggg gattaacgag
     6241 acgttgttta tggcgttgat tacgctggag tctcaggaaa accacagtat tcagccttct
     6301 gaattaagtt gtgctcttgg atcatcccgt accaacgcga cgcgtattgc cgatgaactg
     6361 gaaaaacgcg gttggatcga acgtcgtgaa agcgataacg atcgccgctg cctgcatctg
     6421 caattaacgg aaaaaggtca cgagtttttg cgcgaggttt taccaccgca gcataactgc
     6481 ctgcatcaac tctggtccgc gctcagcaca acagaaaaag atcagctcga gcaaatcacc
     6541 cgcaaattgc tctcccgtct cgaccagatg gaacaagacg gtgtggttct cgaagcgatg
     6601 agctaacgcg tcatctcgct caaaaatcca gatttatata agaaaaaatg accggccagc
     6661 atcgcaacat gctggccttt ttggcaagca ggtcggctca gccgatgagt taagaagatc
     6721 gtggagaaca atatgagcgc aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag
     6781 agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc tctttataat tattgccgta
     6841 gcgataggga tttattggtt tttggtactg cgtcatttcg aagaaaccga tgacgcatac
     6901 gtggcaggga atcagattca aattatgtct caggtgtctg gcagcgtgac gaaagtctgg
     6961 gccgataaca ccgattttgt aaaagaaggc gatgttctgg tcactctcga cccgacagat
     7021 gctcgccagg cgtttgaaaa agccaaaact gcactggctt ccagcgttcg ccaaacccac
     7081 cagctgatga ttaacagcaa acagttgcag gcgaatattg aggtgcaaaa aatcgccctc
     7141 gcgaaagcac aaagcgacta caaccgccgt gtgccgctgg gcaatgccaa cctgattggt
     7201 cgcgaagagc tgcaacacgc ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc
     7261 gctattcaac aatacaatgc caatcaggcg atgattctgg ggactaaact ggaagatcag
     7321 cctgccgtgc aacaggctgc caccgaagta cgtaacgcct ggctggcgct ggagcgtacc
     7381 cgtattgtca gtccgatgac cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa
     7441 attagcccaa cgacgccgct gatggcggtc gttccagcca ccaatatgtg ggtggatgcc
     7501 aactttaaag agacgcagat tgccaatatg cgtatcggtc agccggtcac tatcaccacc
     7561 gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca
     7621 ggtagcgcat tctcactgct tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt
     7681 cagcgtctac ctgtgcgtat cgaactggac cagaaacagc tggagcaata cccgctgcgc
     7741 attggtttgt ccacgctggt gagcgtcaat accactaacc gtgacggtca ggtactggca
     7801 aataaagtac gttcaacacc ggtcgcggta agtaccgcgc gtgaaatcag cctggcacct
     7861 gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt
     7921 gatgcaacag caaaaaccgc tggaaggcgc gcaactggtc attatgacga ttgcgctgtc
     7981 actggcgaca ttcatgcagg tgctggactc caccattgct aacgtggcga tccccactat
     8041 cgccgggaat ctgggctcat cgctcagcca gggaacgtgg gtaatcactt ctttcggggt
     8101 ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa
     8161 actgttcctt tggtccacca tcgcctttgc tattgcgtcg tgggcgtgtg gtgtctccag
     8221 cagcctgaat atgctgatct tcttccgcgt gattcagggg attgtcgccg ggccgttgat
     8281 cccgctttcg caaagtctat tgctgaataa ctacccgcca gccaaacgct cgatcgcgct
     8341 ggcgttgtgg tcgatgacgg tgattgtcgc gccaatttgc ggcccgatcc tcggcggtta
     8401 tatcagcgat aattaccact ggggctggat attcttcatc aacgtgccga ttggcgtggc
     8461 ggtggtgttg atgacactgc aaactctgcg cggacgtgaa acccgcaccg aacggcggcg
     8521 gattgatgcc gtggggctgg cactgctggt tattggtatc ggcagcctgc agattatgct
     8581 cgaccgcggt aaagagctgg actggttctc atcacaggaa attatcattc ttaccgtggt
     8641 ggcagtggtg gctatctgct tcctaattgt ctgggagctg accgacgata acccgatagt
     8701 cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc
     8761 gtatatgctc tacttcggcg cgattgttct gctgccgcag ttgttgcagg aggtctacgg
     8821 ttacacggca acgtgggcag gtctggcctc tgcgccggta gggattattc cggtgatcct
     8881 gtcgccgatt atcggccgct tcgcgcataa actggatatg cggcggctgg taaccttcag
     8941 ctttattatg tatgccgtct gcttctactg gcgtgcctat acctttgaac caggtatgga
     9001 ttttggcgcg tcggcctggc cgcagtttat ccaggggttt gcggtggcct gcttctttat
     9061 gccgctgacc accattacgc tgtctggttt gccaccggaa cgactggcgg cggcatcgag
     9121 cctctccaac tttacgcgta cgctggcggg gtctatcggc acgtccatca ccaccaccat
     9181 gtggaccaac cgcgagtcga tgcaccacgc acagttaacc gagtcggtaa acccgtttaa
     9241 cccaaacgcc caggcgatgt acagtcaact ggaagggctt gggatgacgc aacagcaggc
     9301 ctccggctgg attgcccagc agatcaccaa ccaggggctg attatttccg ccaatgagat
     9361 cttctggatg tcagccggga tattcctcgt cctgctgggg ctggtgtggt ttgctaaacc
     9421 gccatttggc gcaggtggcg gcggaggcgg tgcgcactaa gtacaactaa gccagttcat
     9481 ttgaactggc ttttttcaat taattgtgaa gatagtttac tgactaaatg tgcagttcct
     9541 gcaacttctc ttccggcagc gccagttctt cgttgctgtt gatgcgtacg tcacgttcca
     9601 gaatgctacg cgcaatatcc tgcgcttcct gcaacgagtg catctggtaa gtgccacact
     9661 ggtagacgtt cagttccggg atctgattct gatcctgcac tttcagcacg tcttccattg
     9721 ccgctttcca ggcatcagca acacgctgct catctggcgt accaatcaga ctcatataaa
     9781 aaccggtgcg gcagcccatt ggcgagatat cgataatctc tacaccatta ccgttaagat
     9841 ggttacgcat aaaaccagca aacaggtgct ccagggtatg gatccctctt tctggcatca
     9901 cttctttgtt cggcacgcag aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg
     9961 tgttcattgt tttcgccacc cgaactgcag gcgcttccat ccgggtatga tcgactgtga
    10021 agctatctaa caacggcatt tagccacctc cggtaatttt ttttaaaatt ttctgaactc
    10081 tttcttccca ggcgagtctg agtatatgaa agacgcgcat ttgttatcat catccctgaa
    10141 ttcagagatg aaattttggc cactcacgag tggccttttt cttttctgtc aggcgtgttt
    10201 ttccagccac accgcaaacg gttcggtatc agcggtttcc atttcctgct gacggcgttc
    10261 agatgcctcg cgctcggcta caaaatcctc ttcgcgcaga atttccagcg gctcttcacg
    10321 cagcagatta cggtaggctt ctgcaaacgc tttgcctgtt ccgccaatac cagtatcaat
    10381 catagacctt aagatacggg cagagaaagt cagatcggga ttatcgaagc aggcaaccag
    10441 ttcatcacac actttctgat acgcttcgcc gccgttaatg ctatccagcg tttgcgcgac
    10501 gcgtttcaga tcgcggaaca gatctttacc cacctgcagt aacgggaact gcgcggtttc
    10561 gcagccgata cccagcgtca ggcccggttt gcgaccttca aggatcaccc ggttccagtt
    10621 aacgcgcgta caggcaagtt cgctactgct catttccggt gcatcagcca gcgcacacca
    10681 gaccataaac aggtcgagga atcgcacctg ctgttcatct acaccaatcg gcgagaacgg
    10741 gttgatgtcc agcgaacgca cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga
    10801 aggtgactcg ccgctgcggg taacgcgttt tggacgaatc ggcgcataca gttcgttttc
    10861 aatctgcagc acgttgctat tgatttgcag cctcttaccg tctttctcaa taccaatctt
    10921 cgcgtactct tccgatggcg ttttgattgc ctgtttaagg cccgctacgt actcgtaaag
    10981 atcgttgaag gtaataccaa gattgctttg cgatttattg gtatagccga gatcgctcaa
    11041 acgaagagag gtcgcatacg gcaggtaata cataccgcac tcggttttct caaacggcag
    11101 cgacgttggt tttccttgca ggaaagaaga acaaatcgcc ggagatgcac caaacagata
    11161 aggaatgacc caaccgaaac gatagtaatt gcggataacg cggaaatagc ccgcagaaat
    11221 tttctctttg gcatcagcgc ccgagatatc accgcacttc gcttgccaga atgccattgg
    11281 caaagagaaa ttgtagtgca cgccggaaat ggtttgcatc agcgcgccgt agcgattttt
    11341 cagcccttca cgatacagcg ttttaaagcg tccggtgtta gaagtgccgt actgtgccag
    11401 ttcgatgtcc tgaccttctg cgatgtagca tggcatactt aacggccaca tccgctcatc
    11461 gcccatattg cgcgccgtat aacgatgcag atcgcgcata aaggtcaaca tatgttcaat
    11521 atcaccatcc actggtgtaa tgaattccag caatgcttcc gcaaaatcgg tagtaatcca
    11581 tttgtgcgtc agtgcggaac ctaatgcttc aggatgacct gttgttgcca gtgtgccatc
    11641 agcattaaca cgcaaagttt cgcgctccag cccacgctgt atccccttta acgcctgagg
    11701 atgtttttcc agccaggcca gcgcctgtga tacgtccggg atcaaattga cctcccgcct
    11761 gtcaaaatcg ttttaattag cataactgta atggtgacca tatgtgcagg cctacaatta
    11821 gtgccaccac atcatgccct gaacggtagc tgctgcaact gcaacatagc gtaacgcttt
    11881 accaaggcac aaaaaaaaga ttaccggtcc ccacgagatg cgcatccatc ccgctaacag
    11941 acacaataaa tcgccaacca cgggcatcca gcttaataat agcgtgactg caccatagcg
    12001 tttcagccag ccagtagctt tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg
    12061 cccaaggata acgttagtta accctccaag gctattaccc attgttgctg ttaaaactaa
    12121 aacccaggga taactgatcc cggaaagcaa cattgccacc agcacgactt cggagttgcc
    12181 gggtaatagt gtagcgctga gaaaactact ggcaaacaac gagaaaagcg ataacgcttc
    12241 actcacagca agcgaacatc cacggcgtcc atgcctgctg cacgggccgc ctgaataccg
    12301 aaatcggcat cttcaaagac cacacactgc gtcggttgca cgcccatacg ctgcgcgcac
    12361 aacaaaaatg tgtctggcgc gggtttatgg tgtttgacgt gatcagcagc gacgacggcg
    12421 tcaaaataac ggcgtaatcc caggtgcgcc agcaatgcct cagcgatggc gctttcactc
    12481 cccgttccta cagccattgg gcgacgacca tgccaacttt tcaccacttc aacaagagga
    12541 agcggctcga cactatccag cagcatactt cttactgctt ctgttttttc acgcgctaac
    12601 gcatgcgggt cgagatcggc ctgattcagc tcaataattg cctgagcaat acgccaggtg
    12661 ggcgatccat taagcgcaat cattgcctga acatcgtact gaagaccgta gtgccctaat
    12721 acttcgcgcc acgctttacg gtgcgtaggc tccgtatcca ggattgtgcc atccatatca
    12781 aaaattaaac ctgcataacg ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct
    12841 actttagcgt aattgcttga ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat
    12901 ggataaagca gttatttaga aatgagatat ttttgaagga aatttttggg agaagatggt
    12961 gcatccggga ggattactca ctggcgttcg cccttcgggc cgttgctgac gcaacgttat
    13021 cctccctggt gcctgcggtt aataccgcta tctcttcgat accttcattg ctgaaggtta
    13081 cggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt
    13141 actctatcca gctgagctac ggatgcatcg ggaaaattta ttttactgct gatattgata
    13201 tcgctactaa agccatatca agtaagagat ggtgcatccg ggaggattac tcgccttgcg
    13261 gctcgccctt cgggccgttg ctaaagcaac gttatcatcc ctggtgcctg cggttaattc
    13321 cgctacctct tcgatacctt cattgctgaa gatttcggag aatatggtgc atccgggagg
    13381 attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg
    13441 catcgggaaa atttatttta ctgctgatat tgataccgct actaaagcca tatcaagtaa
    13501 gagatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc cgagtactct
    13561 atccagctga gctacggatg catcgggaaa cttactttac tgcagatttt ttgataccgc
    13621 tactaaagcc gtatcaagta agagatggtg catccgggag gattactcgc cttgcggctc
    13681 gcccttcggg ccgttgctaa agcaacgtta tcctccctgg tgcctgcggt tataccgcca
    13741 cctcttccat accttcatcg ctgaaagtac gtagaatatg gtgcatccgg gaggattcga
    13801 acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcaaatg
    13861 gcggtgaggc ggggattcga accccggatg cagcttttga ccgcatactc ccttagcagg
    13921 ggagcgcctt cagcctctcg gccacctcac cacacgcctc ttacgagtgc ttcgaagaac
    13981 ttgtttatcg ctcatcgtcg ctgcgtggcg cacatattac tttctgagac ttataagtca
    14041 aacaattttt cctacacttt tatcgtttgc acacttcacg ttcaattagt ctgtaaaaac
    14101 gtcaaaaaga gtgttttatc aacagaaaaa tggaggtctg acagatagta gtaatgcaaa
    14161 aaaatggaga cttaagttga atgaacggga gtaaagcgaa aagactatag agtgaaggag
    14221 aaattttgag ggtgcgtctc accgataaag atgagacgcg gaaagattag taactggact
    14281 gctgggattt ttcagcctgg atacgctggt agatctcttc acggtgaaca gaaacttcct
    14341 tcggggcatt tacgccaata cgtacctggt tgccctttac ccctaaaact gtcacggtga
    14401 cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc attctttgct
    14461 ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg cattatccat catataacgc
    14521 caaaaagtaa gcaatgacaa acacattaca tgtaagcagt catggcatta cattctgtta
    14581 aacctaagtt tagccgatat acacaacttc aacctgactt tatcgttgtc gatagcgtta
    14641 atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg cttatattat tgcaatttcg
    14701 cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac
    14761 cggcttgcgc catgtcagga cgtccaccac ccttgccgcc cacctgctga gcgaccatac
    14821 caatcagttc ccctgctttc acacgatctg tgacgtcctt agatacgcct gcaatcagag
    14881 aaaccttacc ttcggctacc gttgccagca cgataattgt cgaccccagc tgatttttta
    14941 aatcgtcaac catggtacgc aacattttcg gctcaacacc gctaagctcg ctaaccaaca
    15001 gcttaacacc attaacatca attgccttac tggaaagatt tgcgctctcc tgtgcggcag
    15061 cttgttcttt aagctgttgt agttcttttt ctaactgacg cgtacgttcc agtactgagc
    15121 gcactttatc cgccagatta ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc
    15181 gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc ggttaccgct tcgatacgac
    15241 gaacgcctgc agcagtaccc gattcagaga tgatgcggaa cagaccaata tcaccagtgc
    15301 ggctggcgtg agtaccgcca cacagctcgg tggagaaatc gcccatgctc agcacgcgta
    15361 cacgctcatc atacttctcg ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga
    15421 gatccatgat gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc accaggtctt
    15481 cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca
    15541 ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt ccccagaacc tggcgcagcg
    15601 cagcgtgcat caggtgcgtt gcggagtgat tcagacgaat acgggcgcga cgagcctcat
    15661 caacatcagc ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt
    15721 gaccaatcgc ctggccgtat ttctgcgtat cttccaccgc gaaggagaag ttagcgcctt
    15781 tcagttcgcc tttatcacca acctgaccgc cggattccgc atagaatggt gtttgatcca
    15841 gcacgaccac agcttcctgg cctgcattga ttgcatcaac cgctttacca tcaacaaaca
    15901 gcgcggtcac tttgccgttc agttccagat ggtcatagcc tttaaattca gatgcactat
    15961 caacacggat cattgcgttg taatcggcac caaagccgct ggcttcgcgc gcgcgacgac
    16021 gctgctcttc cattgctgct tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac
    16081 aaacatcagc cgtcaggtca accgggaagc cataggtgtc gtacagacgg aaagccgttt
    16141 caccatccag cgtatcacca gaaagttttg ccagctcttc atccagcaac gccagaccgc
    16201 gctccagagt acgggcaaac tgctcttctt cagtcttcag cacctgctca acctgcgcct
    16261 gctggcgttt caggtcttca cccgcagagc ccataacgtc gatcagcgga ccaaccagtt
    16321 tgtagaagaa ggtttctttc gcgccgagca tattgccgtg acgcactgcg cgacgaatga
    16381 tacgacgcag tacataacca cggttttcat tggacggcat tacgccatcc gcgatcagga
    16441 acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga tttattgctc agatcggtcg
    16501 cgccagtgac tttcgctacc gcctggatca acgtgcggaa caggtcgatg tcatagttag
    16561 agttaacgtg ttgcagcacc gcagcaatac gctccagacc cataccggta tctacagacg
    16621 gcttcggcag cggttccatc gtgccatctg cctggcggtt gaactgcatg aagacgatgt
    16681 tccagatctc aatgtagcgg tcgccgtctt cttccggact tcccggaggg ccaccccaaa
    16741 tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc gcacggacca gtgtcaccca
    16801 tctgccagaa gttgtcagat gcgtatggcg cacctttgtt atcgccgatg cgaataatac
    16861 gttcgcgcgg gatcccgact tctttttccc agatttcgta ggcttcgtcg tcgctttcat
    16921 agacggtaac ccacagacgc tctttcggca gggcaaacca tttttcgctg gtcagcagtt
    16981 cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag ttgcccagca
    17041 tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac
    17101 cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc
    17161 caaggaacac atccttgaac tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt
    17221 tatggggtac cagggagctg ctggcaacta cctgatgtcc cttactatgg aaaaagtcga
    17281 gaaacgcctg acggatctca gcggtgctct tgctcataat tatcctgaaa tcaagctaac
    17341 gaaatatcgc caccagctcc agcgtgtctt aaccgccggg ctggtaactg aaaagtggga
    17401 ataagataag ttttcttgac tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt
    17461 cgccaaatat cctggatatc ttccatcaga tagccacgat agagcaaaaa acgctggatc
    17521 ttaacttttt ctgaaaagac agttggcaaa ggttcaccat attttcgcgt cgcctgatcg
    17581 cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg
    17641 gaaatacctt tctgattcag ttcctggcga atacgcgcag gtccataacc tttgcggcta
    17701 cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg ttcatggcac
    17761 caggcaataa cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg gccatttttg
    17821 cccataatcg gtgccgcgag tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc
    17881 agaatgcgta ccgcacgatc caacaggcga gcatatgccg ggcgacggga cgttgattct
    17941 gtcatggcat atccttacaa cttaaaaaag caaaagggcc gcagatgcga cccaggtttt
    18001 gctgataaac agatttaata gtcaatgagt gtgcagaatg ttcccgcagg cccaattcgt
    18061 tagaggatcg aggtgagagt aatttttcac gccagtgctg gcctgttccc ctcaccctaa
    18121 ccctctcccc aaaggggcga ggggaccgtc cgtgcacaca ttatctatta tcatcaataa
    18181 cgaacgatta aaaatcttcg ttagtttctg ctacgccttc gctgtcatct acagagaaat
    18241 ccggcgttga gttcgggttg ctcagcagca actcacgtac tttcttctcg atctctttcg
    18301 cggtttccgg gttatctttc agccaggcag ttgcattcgc tttaccctga ccgatcttct
    18361 cacctttgta gctgtaccac gcgcctgctt tctcgatcag cttctctttt acgcccaggt
    18421 caaccagttc gccgtagaag ttgatacctt cgccgtagag gatctgaaat tcagcctgtt
    18481 taaacggtgc agcgattttg ttcttcacca ctttcacgcg ggtttcgcta cccaccacgt
    18541 tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag acgaacagag gcgtagaatt
    18601 tcagcgcgtt accaccggta gtggtttccg ggttaccgaa catcacacca attttcatac
    18661 ggatctggtt gatgaagatc agcagcgtgt tggactgctt caggttaccc gccagcttac
    18721 gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt
    18781 cgatttccgc tttcggcgtc agtgccgcca cggagtcaac gacgataacg tctactgcgc
    18841 cagaacgcgc cagggcgtca cagatttcca gtgcctgctc gccggtgtcc ggctgggagc
    18901 acagcaggtt atcgatatcg acgcccagtt tacgtgcgta gattgggtcc agcgcgtgtt
    18961 cagcatcgat aaacgcacag gttttacctt cacgctgcgc tgcggcgatc acctgcaacg
    19021 tcagcgtggt tttaccggaa gattccggtc cgtagatttc gacgatacgg cccatcggta
    19081 gaccacctgc cccaagcgcg atatccagtg aaagcgaacc ggtagagatg gtttccacat
    19141 ccatggaacg gtcttcaccc aggcgcatga tggagccttt accaaattgt ttctcaatct
    19201 ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc gatagccatt tttactcctg
    19261 tcatgccggg taataccgga tagtcaatat gttctgttga agcaattata ctgtatgctc
    19321 atacagtatc aagtgttttg tagaaattgt tgccacaagg tctgcaatgc atacgcagta
    19381 gcctgacgac gcaccgcatc acggtcgccg ctgaagcatt cccgccgggt aatgccttca
    19441 ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag gcttctcttc actgccgcca
    19501 tccggcccgg cgataccact aatagacacg gcataatcag cacgagccgc tttcagtgcg
    19561 cctatcacca tttccaccac gacgggttca ctcaccgcgc catgctgcgc cagcgtctct
    19621 tcgcgtacgc cgatcatctg cgctttggct tcgttactgt aggtgacaaa tccgcgttca
    19681 aaccaggcgg agctaccggc aatatcggta atcactttcg ctacccaacc accggtacaa
    19741 gactcggcag ttgttacggt tgcgccacgg gctttcagcg cctgcccaac ctgttcactt
    19801 aactgcatca gttcactgtc agtcatcaca acctctgtca gtcaaaaatt tatgccggac
    19861 aagatagcac tttcgtcagg aagatggtgt tggcgttgca attttacgag gaggattcag
    19921 aaaaaagctg attagccaga gggaagctca cgcccccctc ttgcaaatag ttactgcacg
    19981 cgcgccagcg ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg ggtgctgtgg
    20041 ttataacggg taatggtgta gaagttcggc agaccgtacc agtactggta accggtaccg
    20101 acatccagac gcagcaggct ggcttgttga tggttgccca gcggctgctg tggcgttaaa
    20161 cctgccgtgg caagctgcga aatgctgtac ctggttttga agccatttgg caagcctgga
    20221 gcctgaccgt ttgccattac cgcgacctga tcgcctttca cccagccgtg cgctttgaaa
    20281 taattcgcca cgctaccgat cgcatcaacc ggatcccaca ggttgatatg cccgtcgccg
    20341 ctgaaatcta ccgcatattg tttgtaagac gacggcataa actgtccgta gcccatcgcc
    20401 ccggcaaagg aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc catcagcagg
    20461 aaggtttcca gctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt
    20521 gccagcgcat cgaggatgcg agttttcccc atcacgcgcc cccagcgggt ttcaacgccg
    20581 ataatcccga cgataatttc cggcggtacg ccatacacct gccacgcgcg attcaacgca
    20641 tcttcatact gattccagaa aaccacaccg ttctgcacgt tgtccggcgt aataaatttt
    20701 ttgcgataac gcagccatgc gccgttcgga cctgatggcg gtttcaccga tgtggttggt
    20761 gcctggttat ccatcagccg cagtaccgaa tccagacgct tcgcctggga gagaatttcc
    20821 tgcaactgct gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa ctgctgggca
    20881 ttcgggttat tagcgaaatc gccgcccatc tgcatcacat tgtgctgcgg ctcaagcagg
    20941 aagccgccag acggcgttcc ggtggtcgtt tcagtctcag taggttttgg cttgctgcta
    21001 caggcggcaa gcaacacaaa aaggggaagc aatgttacat aacgacgctt gaacatgagg
    21061 ggtccattta acagattcaa ccaggggcaa gtatggtaaa gcatcacgcc ccacacaagg
    21121 aagcggtagt cactgcccga tacggacttt acataactca actcattccc ctcgctatcc
    21181 ttttactcaa actttcaaat taaaatattt atctttcatt ttgagatcaa aataacactt
    21241 ttaaatcttt caatctgatt agattaggtt gccgtttggt aataaaacaa taaatcctga
    21301 aggagagaac aatgatagaa accattactc atggtgcaga gtggtttatc gggctgttcc
    21361 aaaagggcgg agaggtgttt accgggatgg tgaccggcat tcttccgctg ttgattagcc
    21421 tgctggttat catgaacgca ctgattaatt ttatcggtca gcatcgtatt gaacgttttg
    21481 ctcaacgttg cgccggtaac cctgtttccc gttacctact gttaccgtgc attggcacgt
    21541 ttgtcttttg caatccgatg accttaagcc tgggtcgctt tatgccggaa aagtacaaac
    21601 ccagctacta cgcggcggcc tcttatagct gccactcaat gaatggcctc ttcccccata
    21661 tcaaccctgg cgaactgttt gtttatcttg gcattgccag cggtctgaca acgctgaacc
    21721 tgccacttgg cccactggcg gtgagttatc tgctggttgg tctggtcacc aatttcttcc
    21781 gcggctgggt gaccgatctg accaccgcca tttttgagaa aaagatgggc attcaacttg
    21841 aacaaaaagt tcacctggca ggagcaacat catgacgcat attcggatcg aaaaaggaac
    21901 gggtggctgg ggcggcccgc ttgagctgaa agccacgccg ggaaaaaaaa tcgtctatat
    21961 caccgccggt acccggcctg cgattgttga caaactggca cagcttactg gctggcaggc
    22021 tattgacgga tttaaagaag gtgaacccgc ggaggcggaa attggtgtcg cggtaatcga
    22081 ctgtggcggc acattacgct gcggcatcta tccgaaacga cgtattccca ccattaatat
    22141 ccactcgacg ggcaagtccg gtccgctggc gcagtacatt gtggaagata tttatgtctc
    22201 tggcgtaaaa gaagaaaaca tcactgtagt gggtgatgcg acaccacaac cctcttccgt
    22261 gggccgtgac tatgacacca gtaagaaaat caccgaacaa agcgatggtt tactggcgaa
    22321 ggtgggaatg ggcatggggt ccgccgttgc ggtgctgttt caatctggtc gtgacaccat
    22381 cgacactgta ttaaaaacca ttctgccgtt tatggcattc gtctcggcgc tcattggcat
    22441 cattatggct tctggccttg gtgactggat tgcccacggt cttgctccgc tggcgagcca
    22501 tccactgggt ctggtcatgc tggcgctcat ctgctccttc ccactgcttt cacctttcct
    22561 cggcccaggc gcagttatcg cacaggttat cggcgtattg attggcgtgc agattggtct
    22621 cggcaatatt ccgccgcatc tggctttacc ggcactgttt gccatcaacg cgcaggcggc
    22681 ctgcgacttc atcccggtcg gtttgtcgct ggcggaagcc cgtcaggaca cggttcgcgt
    22741 cggtgtccct tctgtactgg tgagccgctt tttaaccggc gcaccgactg tactgatcgc
    22801 ctggtttgtc tccggtttta tctatcaata gaggctgaaa catgaccgtt atttatcaga
    22861 ccaccatcac ccgtatcggc gcgagtgcca ctgacgccct cagcgaccag atgctcatca
    22921 cctttcgtga aggcgcgcca gcggacctcg aagagtattg cttcattcat tgccacggcg
    22981 agttgaaagg tgcactccat cccggtttgc aatttacgct cgggcagcat cgctatccgg
    23041 tgaccgccgt tggcagcgtg gcggaagaca accttcgcga actgggtcat gtcaccctgc
    23101 gcttcgatgg tttaaacgaa gcggaatttc cgggcactgt ccatgtggca ggccctgtac
    23161 ccgacgatat cgcgccggga tcggttttga agtttgaatc tgttaaggag taaaaaatga
    23221 atcaggttgc cgttgtcatc ggtggtgggc aaacattagg cgcgttcctg tgccacggtc
    23281 tggctgccga ggggtatcgc gtcgcggttg tcgatattca gagcgacaaa gccgcaaatg
    23341 tggcacaaga aattaacgcc gaatatggtg aaagtatggc gtacggtttt ggtgctgacg
    23401 ccactagcga gcaaagcgtt ctggcgctct ctcgtggggt agatgaaatc tttggtcgcg
    23461 tggatttgct ggtctatagc gccggaatag caaaagcagc ctttatcagc gacttccagc
    23521 tcggcgattt tgaccgttcg ctacaagtga atctggtggg ttatttcctg tgtgcccgtg
    23581 aattttcgcg tttgatgatc cgcgacggga ttcagggtcg cattattcag atcaactcga
    23641 aatccggcaa agtgggcagc aaacacaact ctggctacag cgcagcgaaa tttggtggcg
    23701 tcgggctgac tcaatcactg gcgctggatc tggcggagta cggcattacg gtgcattcac
    23761 tgatgctcgg taacctgctg aaatcgccga tgttccagtc actgttgcca caatacgcga
    23821 ccaagctggg tatcaaaccg gatcaagtcg agcagtatta catcgacaaa gtaccgctca
    23881 aacgcggctg cgattatcag gacgtgctga atatgctgct gttctacgcc agtcctaagg
    23941 cgtcgtactg caccggacag tcgatcaatg tcaccggcgg tcaggtgatg ttctgattaa
    24001 cagcggagat ccgttaagga tctccgtgag actatagaat gcctgatgcg ctacgctcat
    24061 caggcataca ggacttccgc cactacatta aggaaaagtt atggtatccg cactcatcac
    24121 cgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt
    24181 taaccgtgcc ttcgacacac tatgccagca agggcgggtt ggcgtgggcc gttccagcgg
    24241 gcgctttaaa ccgcgggtcg tggtcgccat cgcgctggac gatcagcagc gcatcgtcga
    24301 caccttgttt atgaaaggac tgaccgtctt cgcccgaccg caaaaaattc ccgcaattac
    24361 cggtatgcat gcgggtgatt tacagcccga tgtgatcttt ccccatgatc cactatcaca
    24421 gaatgctcta tcattggcgc ttaaactgaa acgtggataa tttcgttgtg aatgttactt
    24481 gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc acgcccatga aacctcgtca
    24541 gcgtcaggcc gccattctgg agtatctgca aaagcagggt aaatgctcgg ttgaagaatt
    24601 ggcgcaatac tttgacacca caggcacaac cattcgcaaa gatctggtca ttctggaaca
    24661 tgccggaacc gtcattcgta cttatggcgg agtggtgttg aataaagagg aatccgatcc
    24721 gcctatcgat cataaaacgc tcatcaacac ccacaagaaa gagctgattg cagaagctgc
    24781 cgttagtttt atccatgatg gcgattcgat cattcttgat gctggcagta ccgttttgca
    24841 gatggttccc ctgctctcgc gctttaataa catcacggtg atgaccaaca gcctgcatat
    24901 cgtcaatgcg ctatccgaac tggataacga acaaactatc ctgatgccag gcggaacgtt
    24961 tcgtaaaaaa tcggcctcat ttcacggaca gctggcagag aatgccttcg aacatttcag
    25021 tttcgataaa ttgtttatgg gcaccgacgg catcgatctc aatgcgggcg taaccacctt
    25081 caacgaggtt tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag tgattttgat
    25141 ggcggactca tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc ttgaaagcgt
    25201 cgataagctg attaccgacg caggtatcga tccggcgttt cgtcaggcgc tggaagagaa
    25261 agggatcgat gtgatcataa ccggagagag caatgagtga agcactactg aacgcgggac
    25321 gtcagacgtt aatgctggag ttgcaggaag caagccgttt accggaacgt ctgggcgatg
    25381 attttgttcg cgccgccaat atcatcctgc actgcgaagg caaagtggtg gtttcgggaa
    25441 ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac acttgccagt accggcacac
    25501 cggctttttt tgtccatccg gcagaagcgc tgcacggcga tctggggatg attgaaagcc
    25561 gcgatgtgat gctgtttatc tcttactccg gtggcgcgaa ggaactggat ctgattattc
    25621 cgcgtctgga agataaatct atcgcgctgc tggcgatgac cggcaaaccg acgtcaccgc
    25681 tgggcctggc ggcgaaagcg gtgctggata tctccgtaga acgcgaagcc tgcccgatgc
    25741 accttgcgcc gacctccagc accgtcaata ccctgatgat gggtgacgcg ctggcgatgg
    25801 cggtcatgca ggcgcgcgga tttaatgaag aagattttgc ccgctcccac ccagccgggg
    25861 cactgggcgc tcgcttgctg aataaagtgc atcatctgat gcgccgtgac gatgccatcc
    25921 cacaggtggc gttaaccgcc agcgtgatgg atgcgatgct ggaactcagc cgcaccggtc
    25981 tggggctggt ggcggtatgt gacgctcaac aacaggtaca aggcgtcttt accgacggcg
    26041 atttacgtcg ctggctggtt ggcggcggcg cactcaccac gccagttaat gaagcgatga
    26101 cggtcggcgg caccacgttg caatcgcaaa gtcgcgccat cgacgccaaa gagatcctga
    26161 tgaagcgcaa aatcactgcc gcaccggtgg tagatgaaaa cggcaaactc accggcgcaa
    26221 ttaacctgca ggatttctat caggccggga ttatttaatc cttcattccc agacgtttcg
    26281 ccagccgatg caggttggcg acgtcggttt ccagcatccg cgcgcaggca gcccagttgt
    26341 gatgattttg tgccagcgcc tgacgaatag tttcacgctg gaacgcttct gtcgcttcac
    26401 gcaggttttg cttaacaacg ggcaccgccg ccacttctgg cgtcggcaac gtcacctcag
    26461 gaaaagcaaa atgttgcgcc tcaagaatca cttcatcgcc gttgcgggtg gctctcgaca
    26521 gcactaccgc ccgatgaata gcatgttcca gttcgcgcac gttccccgga aaacgatagt
    26581 gttgcagtaa atttcgcgct ccggcactta ataccacgcg ggagagcccc agccgcaaac
    26641 gacactgctc gcagaaatac cccgccagca gaatgacatc atcgccccgc tcacgcagcg
    26701 gcggcaccga aagtggaaac acgctcaggc gatgaaacaa gtcagcgcga aatcgccctg
    26761 ccagcacctc ttcgcgtaag tcgcggttag tcgccgccag cacgcgcaca tcgacccgca
    26821 aactgcggtc atcgccaacg cgctgaatat cgccatactg caacaccctc agcagcttgg
    26881 cctgcaatgc caacgacaac tcgccgatct catccagaaa cagagtgccg ttatccgcca
    26941 tttcaaactt cccgctgcgg ttactgatag cgccagtaaa cgctcctttc acatgcccga
    27001 acagttcact ttccgccaca ctttccggca gtgcggcaca gttgagatag accagcggat
    27061 tcaccgcccg tggcgaggct tcatgaatcg ctttcgccac cagctcctta ccggttcccg
    27121 tctcaccgct gatcaggacg ttgagatcgg acgccgccac aatctcaatc tcttttttca
    27181 gttgcgtcat gccaggggac aagccaatca tctgcgtctg tttcaccgct tcaaacggcg
    27241 tggcatcgcc tggcatcata ttctggcttt ccagttgttc aatcagcaac gcattgctta
    27301 acgctcccgc cgccagcgca gcaatcagcc gtagctcttc gtcgctgaaa acatcgaact
    27361 gatcgggctg catcccgtcg agcgtcagtg cgccgatcag gttttgcccg gcaaacaatg
    27421 gcagaccaac gcaggcgtga accttcagac tctcctgccc aggaatcaaa ccgtcatagg
    27481 gatcgggcaa ttcgctgtct gcgggaaagc gcaccacatc cccggcgcgg gcaatcgctt
    27541 ccagccgtgg atgcccttcc agcgcaaagc gtctaccgag tacatccttt gccagaccgt
    27601 cgatggcaag cggaataaac tgccgcgaat cgtaacgtag caacgcagac gcatcgcact
    27661 ccagcacctg acgtagcgtg gtgatcaggc gctgaaaacg atcctgatga ccaatcccac
    27721 gctgcaattc gatggcgata ttcgccagca catcaacgga aaaactcatc tttgcctcac
    27781 tgtcaatttg actatagata ttgtcatatt gaccatttga ttgatagtca ttttgactac
    27841 tcattaatga gcataatttt atttatagag taaaaacaat cagataaaaa actggcacgc
    27901 aatctgcaat tagcaagaca tctttttaga acacgctgaa taaattgagg ttgctatgtc
    27961 tattgtggtg aaaaataaca ttcattgggt gggtcaacgt gactgggaag tgcgtgattt
    28021 tcacggcacg gaatataaaa cgctgcgcgg cagcagctac aacagctacc tcatccgcga
    28081 agaaaaaaac gtgctgatcg acaccgtcga ccataaattc agccgcgaat ttgtgcagaa
    28141 cctgcgtaat gaaatcgatc tggcggatat cgattacatc gtgattaacc atgcagaaga
    28201 ggaccacgct ggggcgctga ccgaactgat ggcgcaaatt cccgatacgc cgatctactg
    28261 tactgccaac gctatcgact cgataaatgg tcatcaccat catccggagt ggaattttaa
    28321 tgtggtgaaa actggcgaca cgctggatat cggcaacggc aaacagctca tttttgtcga
    28381 aacaccaatg ctgcactggc cggacagcat gatgacttac ctgacaggcg acgcggtgct
    28441 gttcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca acgatgaagt
    28501 ggatcagacg gagcttttcg agcagtgcca gcgttactac gccaatatcc tgacgccgtt
    28561 cagccgcctg gtaacgccga aaattaccga gatcctgggc tttaacttac cagtcgatat
    28621 gatagccact tcccacggcg tggtatggcg cgataacccg acgcaaattg tcgagctgta
    28681 cctgaaatgg gcggctgatt atcaggaaga cagaatcacc attttctacg acaccatgtc
    28741 gaataacacc cgcatgatgg ctgacgctat cgcccagggg attgcggaaa ccgacccacg
    28801 cgtggcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc tgactaatgt
    28861 cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga tgatgccgaa
    28921 aatcgccggg ctggtggaag agatgactgg tttacgcttc cgtaacaaac gcgccagtgc
    28981 tttcggctct cacggctgga gcggcggtgc ggtggatcgt ctttccacgc gcctgcagga
    29041 tgcgggtttc gaaatgtcgc ttagcctgaa agccaaatgg cgaccagacc aggacgctct
    29101 ggagttatgt cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg cgccgctgcc
    29161 gcagagcacg gtgaatacgg tggttaaaga agaaacctct gccaccacga cggctgacct
    29221 cggcccacgg atgcagtgca gcgtctgcca gtggatttac gatccggcaa aaggcgagcc
    29281 aatgcaggac gttgcgccag gaacgccgtg gagtgaagtc ccggataact tcctctgccc
    29341 ggaatgctcc ctcggcaaag acgtctttga cgaactggca tcggaggcaa aatgagtaac
    29401 ggcattgtga tcatcggttc gggcttcgcc gcccgccaac tggtgaaaaa tattcgcaaa
    29461 caggacgcca ctattccatt aaccctgatt gccgccgaca gcatggatga gtacaacaaa
    29521 cctgacctca gccatgttat cagtcagggg caacgtgccg atgaccttac ccgccagacg
    29581 gcgggtgaat ttgccgagca gtttaatctg cacctgtttc cacaaacctg ggtgacggat
    29641 atcgatgccg aagcccgtgt ggtgaaaagc cagaataatc agtggcaata cgacaagcta
    29701 gtactggcaa ccggtgccag tgcctttgtc ccgcctgtgc ctgggcgtga gttaatgctg
    29761 acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga tgcccgacgc
    29821 gtgttgattg ttggcggtgg tttgattggt agcgaactgg cgatggattt ttgtcgtgca
    29881 ggcaaagcgg tcacgctaat cgacaacgct gccagtattc tggcgtcatt aatgccaccg
    29941 gaagtaagca gccgcttgca gcatcggttg acggagatgg gcgttcatct gttgttgaaa
    30001 tctcagttgc aggggctgga aaaaatagat tctggcattc tggcaacgct ggaccgccag
    30061 cgcagtatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga aaccgccctg
    30121 gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta tctgcaaacc
    30181 agtaatgccg atatttacgc gctgggcgat tgcgcggaaa ttaacggtca ggtattgccg
    30241 ttcctccagc cgattcaact tagcgcgatg gtgctggcaa aaaatcttct cggcaataac
    30301 acgccgctga aactaccggc gatgctggtg aaaatcaaaa cgccggaatt accgctgcat
    30361 ctggcaggcg aaacccagcg tcaggattta cgctggcaaa ttaataccga acgccaggga
    30421 atggtggctc gcggtgttga cgatgctgac cagcttcgcg cctttgtggt cagtgaggat
    30481 cggatgaaag aggcatttgg attgttgaaa acattgccga tgtaggtggg ctactgtgcc
    30541 taaaatgtcg gatgcgacgc tggcgcgtct tatccgacct acggggacgc atgtgtaggc
    30601 cggataaggc gtttacgccg catccggcaa tggtgtccaa atgcaacacg ttttatccgt
    30661 tctggacttc acccgctaac caacgcgccg cagcaataac cccctgcccc agagacaaac
    30721 cgccatcacc cgccggtaaa ctctgtggaa agagcaatgt gaaatcagcg agataatgcg
    30781 ccagacgtgc acgcagcaaa cggttatgaa taaccccgcc gctaaatacc agcgtagtga
    30841 taccacgcat cgtggcctgc tcacgcatca acgcggcaaa accctgcgcc agcgcatcat
    30901 gaaacgccca cgcgcgttga ttaaccggtg cctgccagtt cagccactgc tgccagaaag
    30961 tggcgagatc cagttgattg tccaccagcg gcattgtcac cggatgcgtc actccgtggc
    31021 acgaagctgc gagtgcctcc agagcacaag ccgcttcacc ttcataactt aacgtggctg
    31081 gcgcacagcc cagtgccgcc gccacagcat cgaacaaccg cccacacgat gacgccagcg
    31141 gcgcgttaat tccacgctca atggcccgcg ccagcacgct ccagttttgc tgttgcacac
    31201 ttgctgtttc agaataattc tgccactccg gcacaaagcg caggcactgc gccagcaggt
    31261 ttcgccacgg ctgcttcgcc gccaaatcgc cacccggaag cgccactgca ggcaagccgc
    31321 ccaggtgctc acattcgcga tagttcaccc gcagacactc gccgccccac aaagcgccgt
    31381 tctcccccat accgatacca tcgagcgtca aagcaatgac atcaccgcca tccagcggcc
    31441 actgatgctc tgccagacac gccgctgcgt gggcatgatg atgcagcacc gtttgcgtcg
    31501 gcatattcat ttcgctcgcc cactggctgg agacatagcc cggatgcacg tcatgcacaa
    31561 cgtattgcgg agtgaaatcg tagatgtttt gcatcaggcg taacgcttcg cgccactgca
    31621 tctggatgcc atcgtcactt aaatcgccca gatgctgact caacaccgct tgttcaccgc
    31681 gcaccaggca gaaggtgttt ttcagatccg cgccaagaca cagcacaggc ggaacatttt
    31741 taaagcccgg aggcaaaacc agcgcatccg gcacataccc gcgtgaacgg cgcagcattt
    31801 cgccgctttc gcgcaccacc gaatcatcca tccgctgcac gatgtcgcgg ttatgtatca
    31861 agaatccgtc ggcaatgccc tgcaaatcct ccagcgcctg ttcgttgctg atagccggtg
    31921 gtttaccgct caggttgccg gaggtcatca ccagcgggca ttgcagttcc tgtaacagca
    31981 aatgctggag cgggttcgca ggcaacattg ccccgacttc gttaaggtca ggggcgatat
    32041 catcacaaag ctcaggaacg tattttttat ccaccagcac aatcggcgcg gcgggcgtgg
    32101 taagcaactg gcgcgcagcg tctggtaaac cgtcagccac tggcaacatg accgccagcg
    32161 gtttcgccgg gcgatgtttg cgcgcccgaa gtgtcgccac cgcgttactg ttacgtgcat
    32221 cgcaggcaag atgaaatccg ccaatccctt tgatggcgac aatgttgccc atttttaact
    32281 gtgcgatagc cgcctgtaat gccgcctctt gttctgcatg ttcaccatga cttacccatt
    32341 caagatgtgg gccacactcc gggcaggcca ccggctgggc gtggaagcga cgatcgagcg
    32401 ggtcgcggta ctctttgtca caagccggac acaacggaaa cgccgccatc acggtaaacg
    32461 ggcggtcgta aggcatggcg cgaataatgg tgaaacgcgg gccgcagtgg gtacagttga
    32521 taaacggata acgataacgc cgttcgcctg gggtattcat ttcggcaaga caggcagggc
    32581 aagtagcggc atcgggaaca atttgcgtat tcatggtgcc gcctgtgctc tggcgtatag
    32641 tgaactcggt gggcagttgt gaccagatgt acggctcacg ctcgacgcta tcaatacgcg
    32701 ccagcggcgg gcagtgctga tgcaattgaa caagaaacgt ttccgggtct tccagcagcc
    32761 ggacttctac gccatcgccg tcattacaga catcgccgtg aagatttaat tgctgtgcca
    32821 gctgccagac aaacggacga aaaccgacgc cctgcacttt gcctcgaata cgcagttgga
    32881 caccgcaaga tgtgtttttt gccattgagt tattcccgcc atcatgaatt gcgtaacccg
    32941 ccctgccgga cacgactgcg tcgtatccgg cagtctcagg tcggcgatac cgccagctcc
    33001 gtattctacg aatatttccg ggaattcctt tgatgccaga acagttctat aagattttta
    33061 gaacatcagc gccgtacggc gacgtttttc tgcgctcagt tgttcaagtt tattacgatc
    33121 gacacaaatc agcgcatggg tcgggcaagc cgccatacac gccgggccgt cttcgcgatg
    33181 gttgcacagg tcgcatttat tggcttcagc tttgtcagcc cgtacattca gccccgcgcc
    33241 gctgttgcgg atcaccggac gtaccaccac ttccatcgcg ccatacgggc aagccaccac
    33301 gcaggttttg caaccaatgc aacgttcctg catcacatga acaaaccctt tatcacggct
    33361 gatagcacca ttcgggcaga cgttagcgca cggtgcatct tcacactgac ggcagactgt
    33421 cgccgtggaa atgttcacac ctttaatgac atggattcgc ggtaaaaagg tttccggggt
    33481 cagcgatgca cagtcctgat tttcctgatg agaaaccacg cacgccactt cacaggtacg
    33541 gcaaccaata catttactcg cgtcagcaat gatgaaacgg ttcatcaaat tctccagcaa
    33601 tgacagttaa tgcgccgata cattcacaaa tcatgccagt ttttaatttg ctgttattta
    33661 aggaaattaa tttctgtaat gcaggaaaaa agatgtcatc gacactagtg acgatgacat
    33721 gtgatgacaa tgtttatcgc gaaagggcaa tgagtgagtc gcggcggatc agttttccgc
    33781 tgaaggtttt cggcggtgag aaatccccgc catcgagcat aaaaatcagc cgtccaataa
    33841 tttcctgaat catctctgtt accggaattt ttacgctgga gagcgccgga acggtgtagg
    33901 gggcaattgc gatatcatcg aatccgataa ccgacacctg ctctggcacc gctacgccgc
    33961 gctcgtgcaa cgctttgatc gcacctatcg ccatatcgtc gttactggca actaacgcgc
    34021 taaatttagc cccacgttcg agcaacgttt ctaccccttc ggccccgctg gcgggcgtcc
    34081 atttaccgtt agcgataagt ttttcattga gcgcaatacc atgctgcgac agcgcgtctt
    34141 tatacccggc aagacgttca acgctggtgg gggaatccat cgagccggta aggaaagcaa
    34201 tctcctgatg tccggcgttt atcaactctg ccacggcatt aaagctggtc tgtttatgat
    34261 cgcaccagac gctatggctg ctgtttttgc gcaggcggcg attaagcacc attatcggct
    34321 gactgtgcgc gtcaatgatg tcatcaatct catccacgct taaaaaacgc gggtaaatca
    34381 taatggcgtc gcagcgcaaa tccagcagat actgaatcgc ctggcgctct tcttcggcgc
    34441 tgtgtttacc atctgccaat agcaactgcc gccctttctc ttccgccatt cgcgcggcat
    34501 gaaacagtaa ttcactaaaa taaatgccgt gataaagcgt gttggtcact accagcccca
    34561 gcgtctgagt actcttcgcc gacagattgc gcgccagcaa atttgggcgg taaccgctct
    34621 cttctaccgc ctgaaacacg cgatctttag tctcctggct gacgtagcca ttacctgaaa
    34681 gcacgcggga aacggtcgct tttgaaaccc cggcgcgctt cgccacttcc agcatcgtcg
    34741 tcatcatttt catcccttta cacgcaatca acgcagtgta ctgcaccgtt tgccgattgt
    34801 ccttgcgcaa tcggcgggaa aaatattcag gtgaccggtt tcacaaatat aaaaaatgaa
    34861 caattaactc tcttgcttat ttagtgacaa ctattcatga ttttgtgaaa ccggtttcct
    34921 aattccgttt cagcatcggc atttttccgt tacgtcgact gataacaact acatctaccc
    34981 tactgataac aggataaaat ccgatggcca aaaattatgc ggcgctggca cgctcggtga
    35041 tagcggcact gggcggcgtt gataacatct cggcagtcac gcactgtatg acgcgtttgc
    35101 gctttgttat caaagatgac cagcttatcg acagcccgac gttaaaaacc atccccggcg
    35161 tgctcggcgt ggtacgtagt gacaaccagt gtcaggtgat tatcggcaat accgtttcac
    35221 aagcctttca ggaagtcgtc agcctgctgc cgggagatat gcagcccgca cagcccgtgg
    35281 gtaaacccaa actcacgctg cgtcgcattg gtgcggggat cctcgatgcg ctgatcagca
    35341 ccatgtcacc gctgatcccg gcgattatcg gcggatcgat ggtcaaactg ctggcgatga
    35401 tcctcgagat gagcggcgtg ttgacaaaag gatcgccgac cttaaccatt ctgaatgtga
    35461 ttggtgacgg tgctttcttc ttcctgccgc taatggtcgc ggcatctgcc gccatcaaat
    35521 ttaaaaccaa tatgtcgctg gcgattgcca tcgcaggtgt gctggtacat ccgagcttta
    35581 ttgaactgat ggcgaaagcg gcccagggtg aacatgtcga atttgccctg attccggtca
    35641 ccgcggtgaa atatacctac acggtgatcc cggcgctggt gatgacctgg tgcctgtcat
    35701 atatcgaacg ctgggtggat cgcattacac cggctgtgac caaaaacttc ctcaagccaa
    35761 tgctgattgt gttgattgcc gcaccgctgg caatcctgct gattggcccg attggtatct
    35821 ggatcggtag cgccatttcg gcgctggttt acaccattca tggttatctg ggctggcttt
    35881 cagtcgccat tatgggcgcg ctgtggcctc tgctggtaat gaccgggatg caccgcgtct
    35941 ttacgccaac catcattcag accattgccg aaaccggcaa agaagggatg gtcatgccgt
    36001 cagagatcgg cgctaacctg tcgctgggcg gttcatcact ggcggtggcg tggaaaacga
    36061 aaaacccgga actgcgccag acggcgctgg ctgcggcggc atcagccatt atggcgggga
    36121 tttccgaacc ggcgttatac ggcgtggcga tccgcctgaa acgtccgctt atcgccagtc
    36181 ttatcagcgg ttttatttgc ggcgcggttg ccggtatggc ggggcttgcc agccactcaa
    36241 tggcagcgcc ggggttattt accagcgtgc aattcttcga tccggcgaat ccaatgagca
    36301 tcgtctgggt gttcgcggtc atggcgctgg cggtggtgct gtcgtttatc ctcacactat
    36361 tgctcggctt tgaggatatt cctgttgagg aagcggctgc cgaggcgcga aagcatcaga
    36421 gcgcacaacc gaccgtcgcc aaagaagtaa gtcttaattg aggatgaaaa tgtcagtatt
    36481 tccagaaggt tttttatggg gcggcgcgct tgccgccaac cagtctgaag gtgcgttccg
    36541 tgaaggtggc aaaggactga ccacggtcga tatgatcccg cacggcgagc atcgaatggc
    36601 ggtgaaactg gggctggaaa aacgttttca gttgcgcgat gacgagtttt atcccagcca
    36661 tgaggcgacg gatttttatc atcgttataa agaagatatc gccctgatgg cagagatggg
    36721 attcaaggtt ttccgtacct caattgcctg gagccgcctc tttccgcagg gcgatgaaat
    36781 cacgcccaat cagcagggca ttgcttttta ccgttctgtc tttgaagagt gtaaaaagta
    36841 cggtatcgaa ccgctggtca cgttgtgcca cttcgatgtg ccgatgcatc tggtcactga
    36901 atatggctcc tggcgtaacc gcaagctggt ggagtttttc agccgctacg ccagaacctg
    36961 ctttgaagca tttgatggtc tggtgaaata ctggctaacc ttcaatgaaa tcaacattat
    37021 gttgcatagc ccgttctccg gcgcgggtct ggtgtttgaa gaaggtgaaa atcaggatca
    37081 ggtgaaatat caggccgcgc atcaccagct ggttgccagt gcgctagcca ccaaaatcgc
    37141 ccatgaggtt aacccgcaaa atcaggtggg ctgtatgctg gcgggcggta acttctaccc
    37201 ttacagctgc aaaccggaag atgtctgggc ggcgctggag aaagatcggg aaaacctgtt
    37261 ttttatcgat gtgcaggcgc gcggcgcgta tccggcttac tctgcccgcg tatttcgcga
    37321 aaaaggggta accatcaaca aagcaccggg cgatgatgaa atcctgaaaa acaccgtcga
    37381 ttttgtctct ttaagctatt acgcctcgcg ctgcgcctcg gcggagatga acgccaacaa
    37441 tagcagtgcg gcgaacgtag tgaaatcgct gcgtaacccg tatctgcaag tgagcgactg
    37501 gggctggggt attgatccac tcggtctgcg tatcaccatg aacatgatgt acgaccgtta
    37561 tcagaagccg ctgtttctgg tggaaaacgg cctcggcgca aaagatgaac ttgctgccaa
    37621 tggcgagatt aacgatgact atcgcatcag ctatttacgc gaacatatcc gcgcaatggg
    37681 cgaagcgatt gcagatggca ttccgctgat gggctacacc acatggggct gtattgattt
    37741 agtttccgcc tctacgggtg aaatgagcaa acgctacggt tttgtctacg tagaccgtga
    37801 cgacgcaggc aacggcacgc tgacgcgcac gcgtaagaaa tcgttctggt ggtataaaaa
    37861 agtaattgcc agtaatgggg aagatttaga gtaagtaacc gtgccggatg cggcgtgaac
    37921 gccttatccg gcctacatag ttcgtgcgac atgtaggccc gataagcgca gcgcatcggg
    37981 caatctgggg tttggcatca gtcaaaatca aagaaaccat tccggcttaa cgccaaatcg
    38041 ggctgaaagt gccttaatgt gggatattgt cagggatcgt tgtccagaca aaatctgact
    38101 gaccagggat ttagagccga tttcgttctt caaatccgca taggtcagat tatgttgatc
    38161 aatcaacgtg cgaagtaacg ccacgccaac cggcattgcc gcaacggctt tgtcgaactc
    38221 tgcaaacttt tcgttgttgt tttcatactc tgcaatgcgg ctcgccagaa agtcgataag
    38281 tggattggtg tcatcttctt caatcaaata ttcgaccagt tccagtgctt cacgataatc
    38341 atcttctgag tcactgcctc ccagaaaggg gactgcattg accagttctc ttgttgcttt
    38401 aacagcgcgt gcagcattag cagtcattct ttgttctctc tgtagtaacg tgacgtctgt
    38461 ctgcacgtaa tccccctcat agccactctg caaaaagtca gttctggttc accccataaa
    38521 gcacatcccc ctttatcaag caacgctgcg tctgcgccag atcttccgtt tgcgggtcgc
    38581 gcccggcgat gggtttgagg acgagtttac ggttgggggt ataagtaaaa cgcgtgagcg
    38641 cgggatagct taacttgcca aaccagccgt gaacacgctg ccatttggcg tcttttacct
    38701 gatacagact ggttatccag taaccggaat caaactgcat atccagcaat tgcgtgtggc
    38761 cattcctttg taaatccagc aaatcatcaa tgccagtttc acttgcggta taaaaaccat
    38821 cggactgaaa aacacacggg cgaccttgct gattaaaggt catcagaatc aaaaacgcgt
    38881 taggtgccag accatttcct gaaatacccc gccagataac cagatcctga atgccgttac
    38941 gatcaagatc gacacggtaa agttgcgcat tacccgccat attttgcagc acccccacag
    39001 gcacagacca ggatttaccg gcgcgatctt taccgttcaa ttgccattca ctatcatcat
    39061 ttggcatctt ttcaagcgtc acgccagcaa cgccgacttt tctaaaatgc cagttttgct
    39121 cggcatcata aggcatggtc aggagaggtt tcagcgcccg cttttgcgcg tattcgctgt
    39181 cactcatcgg cagatattcg gtaaagctgg tggcctgtgc aggcagcact aagctcaata
    39241 aaaaagcgca catccctgtt ctgatgttca attccctgaa ttccttaatg aaaaactact
    39301 ctacttccac cgctaactgc gcaaatccac catttccttc ccagccttcc agtcgttgat
    39361 aaacggtttc aaccgcatct ttaatcggct gggtcatcgg gtagtaaaag ccgacgatat
    39421 ccggctggat gccgaggaaa atcacttcgc caatatcttc tttcaactgg tcgataaggt
    39481 agttcaacgg catgttatgg gtagtcatca taaacatctc ggcgatatca tccgggtcga
    39541 tgatgcggat ctcgccgggg tttagcccca tatcagtggc gtcgacaatc agcagtcgcg
    39601 tagggcgcag ttcacggatg gcgacgatgt cgttttccgg tgcactaccg ccgtcaatca
    39661 ccacccagtt acctttcggc gcggcggcgc acttttccgc cagcagcggg cctgcgccat
    39721 catcgcccat catgctattg ccaacacaga gtaaaacgtc agtcacgcag tctcctcacc
    39781 atcaaataga tggcgttttc ctgatgaata tcatgcagca tacttagcag cattgtactc
    39841 cattcctgct gctgcgaagt ttgcgctgcc cgggctttat cgaatgcgtt ggccagcatc
    39901 ggcacatggt taatgtcgat gacgatctca ccatatttcg gcacgccttc cattttgcgg
    39961 cgggcttcgc tgcccgcctc cagagtggca atccatgcca gatattcatc ccacgggcag
    40021 gtgagcgccg cttccaggca atcgataacc ccaaggtggt gaccaatcgc caggctgtaa
    40081 tagaccacct gctgcgcctc ggcgggcgtg gcatcgttct catcaataaa tttacggctc
    40141 agttgactga acaccacctt ttcactcatc ggatacgcgc ctcttctaca acatgattca
    40201 gatggctgac aatctcgttc agacgcggat cgttttccgc ttccagccag cgtgcaacct
    40261 gttcttcgcc ctgccctaac tgtgtcagga agtcatcggc aatctgacga ccgtaacgat
    40321 aacctgccag gcggcgtgct tcgcgatcca ctttcacacg cagcggctgc accatatcgc
    40381 catgcaggat ttccgccggt tgttcatcct gctcacccgg gccacgggcg tgaattttct
    40441 gctccagcag gccgagcgcc attgcaaagc catacagcgt ggcggcaggc gttggcgggc
    40501 aaccagggat gtagacatct accgggacga ttttatccgt accgccccac acgcagtaga
    40561 gatcgtggaa gatcccgcca ctgttaccgc aggcaccgta ggagatacag attttcgggt
    40621 ccggcgcgga ctgccacgca cgcagcgcag gggatcgcat tgcacgggtg accgcgccgg
    40681 taaacagtaa aatatccgca tgacgcggtg aaggaacgac tttaatgccg aagcgttctg
    40741 catcaaacag cggcgaaagc gtggcgaaaa tttcgatttc gcagccgttg cagccgccgc
    40801 agtccacgcg gtaaacgtag gcagaacgtt tgattttttt cagtaacgac gccttcatgc
    40861 tggcgatgga ttcatccacc gtcatcggga ccggaatgcc gttggcgtca cgggggccta
    40921 ataaattgct catcagatgg cctctttcat atggcgagtc agttcaatac ggtcggacgg
    40981 caccaggcat ttctggcgct tacattccgg gcaagtctca aagctttcgc ggtggttttc
    41041 cgcgcggctg tcaccattgt gcttaagcag cgcaatggcg tagtcgatct ctttctggac
    41101 ggcgaaagga cgattgcaga cgcgacagct gcacagcaca aaacgggact gttgcaggaa
    41161 atcttctttc ttccacaccg ccagttcgta ctcttgcgac agtttgatcg ccgccgtcgg
    41221 gcaaacttct tcgcagcgtc cacagaagat gcagcgcccg agattaaact cccacgcaag
    41281 ctctcctgtg gcgaggtcgg tttcaaccgt taaagcgttt gacgggcagg cattgacgca
    41341 ggccgcgcag ccgatgcact gctgcgggtt ctgctctggc ttaccacgga agtttttatc
    41401 aaccgcaatc ggctccagcg gataagacga ggtcgccgtg ccggttttga tgactttttt
    41461 gataaaggta aacatggcga ttccttattt cagcggcgag tttttacgct caatgctgta
    41521 acgctcgagt tctttgtacg gcaccacttt gctcttcttc ttacgtacat cgaccaccgt
    41581 catgcggtcg gtacaggagt agcaagggtc caggctaccg ataatcagcg gcgcatcaga
    41641 aacggtgttg ccacgcagca tgtagcgcag ggtcggccag ttcgcgtagg tcgcggcacg
    41701 gcagcgccag cggtacagct tctggttgtc gccggtcatg ctccagtgga tatcgtcgcc
    41761 gcgcggcgct tcggcaaagc ccagcgcgaa acggtgcgga atgtaggtaa agccttccac
    41821 catcaacgga ccgcccggca ggttatccag accgtagtcg atcatgttca gcgcggtata
    41881 gacctcgttg attcgcactt tcagacggga aataacgtcg cagccctgct cgctgtggac
    41941 ttccattggc agcaggccat aaccaacaaa cgggtgatcg gcgcgggtat cgcgagcgtg
    42001 accgctggca cggaccatcg ggccaacgtt actgaagtcg cgggcaattt ccgggtccag
    42061 acgaccaatg ccgacggtgc gctgttccat gttcggcgta ctcagcagca catccaccag
    42121 ctcctgcact tcacgacgca tctgttgcgc cagctggcgg gtctggatca tgtcatcttt
    42181 caacagatcg cgacgaatcc cgccgatcag gttcaggccg taggttttac gcgcaccggt
    42241 gaggatctct gccattttca tggaggtttc acgcacgcgg aagaactgca taaagccgga
    42301 gtcaaagccg gtaaagtggc aggcgagacc gaggttgagc agatgcgagt gcaggcgttc
    42361 cacctccagc agaatggcgc ggatcatctg cgcacgttct ggcacctgaa tacccatcgc
    42421 gttttctacc gacgtggtgt aggcggtgct gtgggcaaag ccgcagatcc cgcacacacg
    42481 gtcagagagg aaggtcactt cgttgtaacc catacgggtt tccgccagtt tttccatgcc
    42541 gcgatgaacg tagaacagac ggtagtcggc gtcgataatg ttttcgccat cgacgaacag
    42601 acggaagtgg cccggttcgt cagaagtgac gtgcagcgga ccaatcggca cgacgttgtt
    42661 tttcttgtcg cccagttcgt tgatgaactc gtaggtttca gcatcggtag tcggtgccgg
    42721 acgctgacga taatccatgc tgtctttacg cagcggataa agttcatccg gccagtcatc
    42781 cggcagcacc agacgacgtt catccggcag accaaccgga atcaaaccgt acatatcgcg
    42841 cacttcgcgc tcgccccaca ccgccgccgg aacgcgcggc gtcacggacg gatactccgg
    42901 tttgttggcg tcaacttcga cgcgaaccgt tacccaacac ttggtgccct tctccatcga
    42961 cagcacgtaa taaacggcgt aatgaccatt cagtttgcgt tcgtcgttac caaacagcac
    43021 cgacagccag ccaccctgct tgtagtagag aaactccacc acttccggca ggtagttcac
    43081 cttcacggtc acggtcagct gatctttggt ctgccaggcg tggtccagca cgacgcccgg
    43141 aaatgcctca ttcagcgcgg cgagataatg ttgacctaat ttttcttcag acatgctcaa
    43201 actctcttta atcacgccgc cagcaaggag acgaacgcta aaaatgcaaa gccaaaccca
    43261 gcccaggtaa tgcgcggagt aatatcaaga cgcagacggg ccatgctgtt ttcgaacagc
    43321 gcgataacca ggacgccgac gaccagtttg acgatggcaa tcaccagcgc cagcagcagc
    43381 ccaccggcgg tgaaggtttc catctgtccc cacgggataa atacgccgac gaacatctgc
    43441 agcaccacca gctgtttcag gctgataccc catttcatga cgccaaagcc gctgccgctg
    43501 tattcagaga gcgggccttc ctgcagctcc tgctcggctt ccgccaggtc gaacggcagt
    43561 ttgcccattt cgataaaggt ggcgaacgca caggcacaaa gcgccagtac cagcgggatg
    43621 ctctggctca gcggccagtg ataaacggtg tcggtgatgt tgctgatgtt ggtggaaccg
    43681 gcaacctgtg cggcgaccca cagaccaagc agcagcatcg gttcgaccag cacgccaagc
    43741 atcgcttcac ggctcgcgcc gatagcggta aacgggctac cggtatccag accggaaatg
    43801 gcaaagaaga aacgcgcgat ggcaaagaga tacagtaagg tgattaaatc acccagttgc
    43861 ggcagcggag aaccgacggt caccaccggc agcgcagtag cgatagtcag catgacgccc
    43921 accatcacat acggcgtcag gcggaacacc cagccggagg catccgggcc aacgctctgg
    43981 cggcccagca gtttgaaaat gtcacgatac tcctgcaaca cacccggccc acgacggtta
    44041 tgcagacggg cgcgcgccac gcgggtaata ccggagagca gcggcgcaac ggcaaataac
    44101 accagcgcct gaattaacgg atataaaaca ctcattctca ggctcctcgt gaaacaataa
    44161 tcaccaccag taccgccagt tcaaccagcg ccatccggcg gaacagcaac gcactacctt
    44221 cgcactgcca gcccggcact agcgacaccg gattcagcca tttgcgcagt ttcagcactg
    44281 gcgcaaacgc ctgtttaacc ggcatggcaa aaccgtgagc ggtaatcacc attgattttt
    44341 catggtcgta gccgcacacc caggccgcac cgcgggaacg tgacggcagg cggtcgcctt
    44401 tgcaaatcgc cataatgatg aatggcagca gcgggcaggc aatcagcagc aatgtgatca
    44461 tgggttgaga aacggtggtg ttagcaggct ccagcggcag aggtacagca gcagagagca
    44521 tcggcagcag ccacggcgca gcaacaccgc caattacgca gcaaatcgcc agtgccacta
    44581 cgcttacgct catcaggagc ggcgcacagg tggcgttttc ggcttctttg gtacgcggcg
    44641 cgccgaggaa cgtgacgcca tagactttcg ccatacacat caccgccagc gcaccggtaa
    44701 ttgccagacc cacagcgagc agcggcccca gcagacgggc aacaaacgcg ccactattgc
    44761 tcagtttgaa aaaggattga tagataaccc attccccggc aaacccgttc agcggcggca
    44821 gcgcagccat tgccatcaac ccgactaaca tggcgatgga gataaccggc attttcttgc
    44881 caataccacc gagtttttcg atatcgcgat gaccggtacg gaaccagacg ctccccgccc
    44941 cgaggaacag tacgcttttg aacaggctat ggttaagcag atggtacaga ccaccgacca
    45001 gaccaagagc aatcagcgcc ggttgttcga gcgcgatacc cgttacgcca gcgcccagcc
    45061 ccagcaggat aatgccgata ttttccaggg tgtggtaagc cagcaggcgc tggatgttgt
    45121 gttccatcaa cgcatacaga ccaccgacga acgcggtgat catgccgagc accagcagcg
    45181 cgatccccca ccacagcggc gcgttaccac ccagcagcga cagggttaaa atgcccagca
    45241 ggccaatttt catgactacc gtagaaaaca acgcggcagc tggcgcagag gcgttagcat
    45301 gtgcctgcgg cacccagccg tgcagcggaa taatcccggc cagcaggcca aagccaatca
    45361 caccgagcag ccagatatcg gaaccgagcg gcagctgttg catacgcata tccagcaggc
    45421 gcagatccag cgtgccataa cgctgccaca gcagccagca agcaatcgcc agcagcagag
    45481 tgccaagacg ccccagcgca aaccacagtt tgccctcttt gctgttgctg gtgaggaaca
    45541 ccgcacacaa ggccatgact tcggccatta ccacgaacat gccgaggttg ctggcaatga
    45601 cggcgcagac ggcggcagcc atcaacatat tgatctgcaa gccgttgcac ttcacctgcg
    45661 cgtggcgatg ccagtcaatg ttatagaggc tgacaaacag accgcaaagg ccgagggtaa
    45721 tcagccaaat cgcgttaagc ggggagattt gcacatcgta gcttaccagc gacagcgcac
    45781 cgctcacgcc aaccgcgcca gtcagcacag tgaagcccgc ggctgccgta tacaggctac
    45841 caaccgcgcc gccaattccg gctatccagc cacttagcgt cttttgaaaa gaaaagagaa
    45901 atgccagaac agcggcggca ataaaccacg ccacgccgct attgatcagg gaaattgcgc
    45961 tcatttagcc tctccacttt gagcctgctg aaacaaggtg agatcgccaa agtccgtatt
    46021 aaaagtcagc tcacgcttac gtttgctgac gcgggcgata tcggtgttat ccaccagatg
    46081 cagggctttg gtcgggcaca tccgcacgca ggccggacct tgttcatcaa aactgcaaag
    46141 gtcacatttg acggcgatcg cgcgaatacc cggcacccag tcaagcagcg tactgatacg
    46201 cgccggagca ggcggtgccg gtggcgcttt cggggtattg gcgtttgccg gaatatccag
    46261 cggacggctg ccggaaaatt caattgcgcc aaacgggcag gcgatgccgc acagcttgca
    46321 gcttacgcac aggctttcat tcaactgcac ggccccatca acgcgggtga tggcgttaac
    46381 cgggcagacc accgcgcagg gtgcatcttc acagtggtga cagagctgcg gcgcagattc
    46441 tttttcattc agcatcactc tcaggcgcgg cattgattgc aggccgtgct ggcgatgcgt
    46501 ctctgaacag gcggcctcac aagtgtggca gccgatacag agcgtggagt cagcaattac
    46561 aaaacgattc accaggcatt cctcaggtga ttgtcatttt tgacgaaaac atgccgatga
    46621 aatgtcattt tcgacactca tcgacacgcc catccccaaa caggcgtaac gcctgcaaaa
    46681 cgggcaaagc ctcagctcat gctgccgggc tttgtccctt taccagttgg cttaaattca
    46741 ccggcacatt gttttcaata gcggtgtata agctgtcgta aacgccagcg attttttcga
    46801 gatagtgttc cagaacctgt tcgcgatcgc ggatgttgac cttgccatca atcatgccgt
    46861 caatgctcag ttgcgcctgc gcaatccgct gtttgtcttc gccatctttt gtttcgacgt
    46921 aatactcgat ggtgtggctg ttaaagccat ccgccagggt gacgtaaatg cgaaaatgtt
    46981 caaaaaggac gaaacagagt tctttgtccg gcgcgcagct catggcgtga tgcaggcgcg
    47041 ctttcgataa cgtgatcccc tgaaccagcg aattgcagta gatgtgccac tggtcctgta
    47101 ggcgacgatg ccgctgtgcg atgtaatcgg ctttctcgct tatttcccaa atagtcattg
    47161 tcaggttacc cgtttaacag agatgccagc tttaagcatt ttctgtgcca acttttaatt
    47221 tattgttatt aaagagattt ttaagctaaa gatgaatttc gtcgccgtgt cgacgtgtca
    47281 tttcgacatc atcgacatta ttcaccgcag ggataatcaa cactggcaca attattgctt
    47341 gtagctggca atagttaatg ggaggcgata tgcacgaaat aaccctctgc caacgggcac
    47401 tggaattgat cgaacagcag gccgcaaaac acggcgcaaa acgcgtaact ggggtctggc
    47461 tcaaaattgg cgcattttct tgtgtcgaaa ccagctctct tgccttttgt tttgatctgg
    47521 tttgccgcgg cagcgtggcg gaaggttgta aactgcacct cgaagaacaa gaggccgaat
    47581 gctggtgtga aacatgccaa cagtatgtga cgctactgac ccagcgcgtc cgccgctgtc
    47641 cacagtgtca tggtgacatg ctgcagattg tggcagacga cggtttacag attcggcgga
    47701 tagaaataga ccaggagtga gcgatgtgta caacatgcgg ttgcggtgaa ggcaacctgt
    47761 atatcgaggg tgatgaacat aaccctcatt ccgcgtttcg tagcgcgcca tttgccccgg
    47821 cggcacgccc gaagatgaaa atcaccggca ttaaagcgcc tgaatttacc cccagccaga
    47881 ctgaagaagg cgacctgcat tacggtcatg gcgaagcggg cactcacgca ccgggcatga
    47941 gccagcgtcg gatgctggaa gtcgaaattg acgtgctgga caaaaataac cgtctggctg
    48001 aacgcaaccg cgcgcgcttt gctgcccgca agcaactggt gctcaacctg gtttccagcc
    48061 caggttccgg taaaaccacc ctgctgacgg aaaccttaat gcgcctgaaa gacagcgttc
    48121 cgtgcgcggt tattgaaggt gaccagcaaa ccgtgaacga tgccgcacgc attcgcgcta
    48181 ccggcacacc agcgattcag gtgaacaccg gtaaaggctg ccatcttgac gcacagatga
    48241 ttgccgacgc cgcaccgcgt ctgccactgg acgataacgg tattctgttt atcgaaaacg
    48301 ttggcaacct cgtatgcccg gccagcttcg atctcggtga aaaacacaaa gtggcggtgc
    48361 tttccgttac cgaaggtgaa gacaaaccgc tgaaatatcc gcatatgttt gccgccgcct
    48421 cgctgatgct gctcaacaaa gttgacctgt tgccgtatct caactttgac gttgagaagt
    48481 gcctcgcctg cgcccgcgaa gtcaatccag aaattgaaat catccttatt tccgccacca
    48541 gcggtgaagg gatggaccag tggctgaact ggctggagac acagcgatgt gcataggcgt
    48601 tcccggccag atccgcacca ttgacggcaa ccaggcaaaa gtcgacgtct gcggcattca
    48661 gcgcgatgtc gatttaacgt tagtcggcag ctgcgatgaa aacggtcagc cgcgcgtggg
    48721 ccagtgggta ctggtacacg ttggctttgc catgagcgta attaatgaag ccgaagcacg
    48781 cgacactctc gacgccttac aaaacatgtt tgacgttgag ccggatgtcg gcgcgctgtt
    48841 gtatggcgag gaaaaataat gcgttttgtt gatgaatatc gcgcgccgga acaggtgatg
    48901 cagttaattg agcatctgcg cgaacgtgct tcacatctct cttacaccgc cgaacgccct
    48961 ctgcggatta tggaagtgtg cggtggtcat acccacgcta tttttaaatt cggcctcgac
    49021 cagttactgc ctgaaaacgt tgagtttatc cacggtccgg ggtgcccggt gtgcgtactg
    49081 ccgatgggta gaatcgacac ctgcgtggag attgccagcc atccggaagt catcttctgt
    49141 acctttggcg acgccatgcg cgtaccaggg aaacagggtt cgctgttgca ggcaaaagca
    49201 cgcggtgccg atgtgcgcat cgtttactcg ccgatggatg cgttgaaact ggcgcaggag
    49261 aatccaaccc gcaaagtggt gttcttcggc ttaggttttg aaaccaccat gccgaccacc
    49321 gccatcactc tgcaacaggc gaaagcgcgt gatgtgcaga atttttactt cttctgccag
    49381 catattacgc ttatcccgac gcttcgcagt ttgctggaac agccggataa cggtatcgac
    49441 gcgttcctcg cgccgggcca cgttagtatg gttatcggca ctgatgccta taattttatc
    49501 gccagcgatt ttcatcgtcc gctggtggtg gctggtttcg aaccgcttga tctactgcaa
    49561 ggcgtggtca tgctggtgga gcagaaaata gcggcccaca gcaaggtaga gaatcagtat
    49621 cgtcgggtgg taccggatgc cggtaacctg ctggcgcaac aggcgattgc cgatgtgttc
    49681 tgtgtcaacg gcgacagcga atggcgcggc ttaggcgtga ttgaatcttc tggtgtacac
    49741 ctgacgccag attatcaacg attcgatgcc gaagcgcatt tccgcccggc accgcagcag
    49801 gtctgcgatg acccgcgcgc acgttgtggt gaagtattaa cgggcaaatg taagccgcat
    49861 caatgcccgc tgtttggtaa cacctgtaat cctcaaaccg catttggtgc actgatggtc
    49921 tcctccgaag gagcgtgcgc cgcgtggtat cagtatcgtc agcaggagag tgaagcgtga
    49981 ataatatcca actcgcccac ggcagcggcg gccaggcgat gcagcaatta atcaacagcc
    50041 tgtttatgga agcctttgcc aacccgtggc tggcagaaca ggaagatcag gcacgtcttg
    50101 agctggcgca gctggtagcg gaaggcgacc gtctggcgtt ctccaccgac agttacgtca
    50161 ttgacccgct gttctttcct ggcggtaata tcggcaagct ggcgatttgc ggcacagcca
    50221 atgacgttgc ggtcagtggc gctattccgc gctatctctc ctgtggcttt atcctcgaag
    50281 aaggattgcc gatggagaca ctgaaagccg tagtgaccag catggcagaa accgcgcgcg
    50341 cggcaggcat tgccatcgtc actggcgata ctaaagtggt gcagcgcggc gcggcagata
    50401 aactgtttat caacaccgct ggcatgggcg caattccggc gaatattcac tggggcgcac
    50461 agacgctaac cgcaggcgat gtattactgg ttagcggcac gctcggggac cacggggcga
    50521 ctatccttaa cctgcgtgag cagctggggc tggatggcga actggtcagc gactgcgcgg
    50581 tactgacgcc gcttattcag acgctgcgtg acattcccgg cgtgaaagcg ctgcgtgatg
    50641 ccacccgtgg tggtgtgaac gcggtagttc atgagttcgc ggcagcctgc ggttgtggta
    50701 ttgaaatttc agaagcggca ctgccggtta aacctgctgt gcgcggcgtt tgtgaactac
    50761 tgggactgga cgcccttaac tttgccaacg aaggcaaact ggtgatcgcc gttgaacgca
    50821 acgcggcaga gcaagtgctg gcagcgttac attcccatcc actggggaaa gacgcggcgc
    50881 tgattggtga agtggtggaa cgtaaaggtg ttcgtcttgc cggtctgtat ggcgtgaaac
    50941 gaaccctcga tttaccacac gccgaaccgc ttccgcgtat atgctaataa aattctaaat
    51001 ctcctatagt tagtcaatga ccttttgcac cgctttgcgg tgctttcctg gaagaacaaa
    51061 atgtcatata caccgatgag tgatctcgga cagcaagagt tgttcgacat cactcggaca
    51121 ctattgcagc agcccgatct ggcctcgctg tgtgaggctc tttcgcaact ggtaaagcgt
    51181 tctgcgctcg ccgacaacgc ggctattgtg ttgtggcaag cgcagactca acgtgcgtct
    51241 tattacgcgt cgcgtgaaaa agacaccccc attaaatatg aagacgaaac tgttctggca
    51301 cacggtccgg tacgcagcat tttgtcgcac cctgatacgc tgcattgcag ttacgaagaa
    51361 ttttgtgaaa cctggccgca gctggccgca ggtgggctat acccaaaatt tggtcactat
    51421 tgcctgatgc cactggcggc ggaagggcat atttttggtg gctgtgaatt tattcgttat
    51481 gacgatcgcc cctggagcga aaaagagttc aatcgtctgc aaacatttac gcagatcgtt
    51541 tctgtcgtca ccgaacaaat ccagagccgc gtcgttaaca atgtcgacta tgagttgtta
    51601 tgccgggaac gcgataactt ccgcatcctg gtcgccatca ccaacgcggt gctttcccgc
    51661 ctggatatgg acgaactggt cagcgaagtc gccaaagaaa tccattacta tttcgacatt
    51721 gacgatatca gtatcgtctt acgcagccac cgtaaaaaca aactcaacat ctactccact
    51781 cactatcttg ataaacaaca tcccgcccac gaacagagcg aagtcgatga agccggaacc
    51841 ctcaccgaac gcgtgttcaa aagtaaagag atgctgctga tcaatctcca cgagcgggac
    51901 gatttagccc cctatgaacg tatgttgttc gacacctggg gcaaccagat tcaaaccttg
    51961 tgcctgttac cgctgatgtc tggcgacacc atgctgggcg tgctgaaact ggcgcaatgc
    52021 gaagagaaag tgtttaccac taccaatctg aatttgctgc gccagattgc cgaacgtgtg
    52081 gcaatcgctg tcgataacgc cctcgcctat caggaaatcc atcgtctgaa agaacggctg
    52141 gttgatgaaa acctcgccct gaccgagcag ctcaacaatg ttgatagtga atttggcgag
    52201 attattggcc gcagcgaagc catgtacagc gtgcttaaac aagttgaaat ggtggcgcaa
    52261 agtgacagta ccgtgctgat cctcggtgaa actggcacgg gtaaagagct gattgcccgc
    52321 gcgatccata atctcagtgg gcgtaataat cgccgcatgg tcaaaatgaa ctgcgcggcg
    52381 atgcctgccg gattgctgga aagcgatctg tttggtcatg agcgtggggc ttttaccggt
    52441 gccagcgccc agcgcattgg tcgttttgaa ctggcggata aaagctctct gttcctcgac
    52501 gaagtgggcg atatgccact ggagttacag ccgaagttgc tgcgtgtatt gcaggaacag
    52561 gagtttgaac gtctcggcag caacaaaatc attcagacgg acgtacgttt aattgccgcg
    52621 actaaccgcg atctgaaaaa aatggtcgcc gaccgagagt tccgtagcga tctctattac
    52681 cgcctgaacg tattcccgat tcacctgccg ccactacgcg agcgtccgga agatattccg
    52741 ctgctggcga aagcctttac cttcaaaatt gcccgtcgtc tggggcgcaa tatcgacagc
    52801 attcctgccg agacgctgcg caccttgagc aacatggagt ggccgggtaa cgtacgcgaa
    52861 ctggaaaacg tcattgagcg cgcggtattg ctaacacgcg gtaacgtgct gcagctgtca
    52921 ttgccagata ttgttttacc ggaacctgaa acgccgcctg ccgcaacggt tgtcgcccag
    52981 gagggcgaag atgaatatca gttgattgtt cgcgtgctga aagaaaccaa cggcgtggtt
    53041 gccgggccta aaggcgctgc gcaacgtctg gggctgaaac gcacgaccct gctgtcacgg
    53101 atgaagcggc tgggaattga taaatcggca ttgatttaac tgcaaattgc cggacagatc
    53161 tgcctgtccg gcatactatt cacgaggttt tttcggacga tatttttccg gcagttctgg
    53221 caccggacgc ttgtcatcga tgagatgacg cacggttaag atcggatgac gccacagcat
    53281 tctcggcccg gcccaacgca taatctgttt catctcttca cgctttgcag gctggtaaca
    53341 gtgcaccgga cactgcttac aggctggttt ctcttcgccg aacacacatt tatccagccg
    53401 cttttgcgcg taaacaaaca acgcctcgta atgctccggc tccgctgacg cctgcgggca
    53461 tttcgcttga taaagatcga tcattttttt aatcgtcagt ttttcacgag agatacgctt
    53521 gccggacatg ctgcctccac ctcattaaga tgtatttata ttacatctta atcttaaagg
    53581 gcactatgac tctaaagaag aaaggttcgc caaccgacgc aattttgcgt acttgcttca
    53641 taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc aagaactcgc
    53701 cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta tgtgattaca
    53761 acgaaaataa aaaccatcac accccattta atatcaggga accggacata accccatgag
    53821 tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca agctgaaagc
    53881 ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac tgttttatga
    53941 cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg gtgcttcggc
    54001 gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact atctcgccaa
    54061 actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc cggcgaccag
    54121 caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca tcagcgatga
    54181 agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg actgcaaagg
    54241 tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg aaccggccga
    54301 ccgcgaaacg atggcggcag agctgcaacg cactaatccg gcggaactgc tgtatgcaga
    54361 agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc gcccgctgtg
    54421 ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca cccgcgatct
    54481 ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt gtctgttgca
    54541 gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca ccatggaacg
    54601 tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa ttactcagaa
    54661 cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg tcacgccgat
    54721 gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc gcgtgttgct
    54781 tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc agccggtact
    54841 gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa ctgctcgccc
    54901 acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc gtgcgcagtt
    54961 agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg agtttgccga
    55021 gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg tacgcgacgg
    55081 tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc tggctgacgg
    55141 cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg gcctggacac
    55201 gctgaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc gtgggcaaag
    55261 ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg agcgctacat
    55321 cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca aagcactggc
    55381 tctggaaaaa cagctttatg aagaactgtt cgacctgctg ttgccgcatc tggaagcgtt
    55441 gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gtgaacctgg cggaacgggc
    55501 ctataccctg aactacacct gcccgacctt tattgataaa ccgggcattc gcattaccga
    55561 aggccgccat ccggtggttg aacaggtgct gaatgagccg tttatcgcca acccgctgaa
    55621 tctgtcgccg cagcgtcgca tgttgatcat caccggtccg aacatgggcg gtaaaagtac
    55681 ctatatgcgc cagaccgcgt tgattgcgct aatggcctat atcggcagct acgtaccggc
    55741 gcaaaaagtc gagattggcc cgattgaccg tatctttacc cgcgtaggcg cggcagatga
    55801 cctggcttcc gggcgttcga cctttatggt ggagatgacc gaaaccgcca atattttaca
    55861 taatgccacc gaatatagtc tggtattgat ggatgagatc gggcgtggaa cgtccactta
    55921 cgatggtctg tcgctggcgt gggcgtgcgc ggaaaatctg gcgaataaga ttaaggcatt
    55981 gacgctgttt gccacccact atttcgagct gacacagtta ccggagaaaa tggaaggcgt
    56041 cgccaacgtg catctcgatg cactggagca cggcgacacc attgccttta tgcacagcgt
    56101 gcaggatggc gcggcgagca aaagctacgg cctggcggtt gctgctctgg cgggcgtacc
    56161 gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca tttcgccgaa
    56221 cgccgccgct acgcaagtgg atggtacgca aatgtctttg ctgtccgtac cagaagaaac
    56281 ttcgcctgcg gtcgaggcac tggaaaacct ggacccggat tcactgactc cgcgtcaggc
    56341 gctggagtgg atttatcgct tgaagagtct ggtgtaataa aaattcccga tagtcttttg
    56401 ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta taataggaaa
    56461 gcttatttta cagggtaaaa ccatgccatc tacacgctat caaaaaatca atgcccatca
    56521 ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat tacaatcccg
    56581 cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg gcgataatat
    56641 tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct ggtttatctc
    56701 cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg atggcaatat
    56761 gtggcttgcc agcggtggtg actggttttt cgatttaaat gattcagagc aaaaagaagc
    56821 tacagatctg ttgctgaaat tccatcacct tccacatatt attgaaatca ctaacgacaa
    56881 cataaaatat gtcatcgcac atgcagatta tccggggagt gaatatctct ttggtaaaga
    56941 aatagcggag agcaaattac tctggcctgt tgatcgtgtg cagaaatcgc ttaatggcga
    57001 gttacaacaa ataaacggcg ctgattattt tatatttgga catatgatgt ttgataacat
    57061 tcagacgttc gctaaccaga tttatattga taccggatcg ccgaaaagtg ggcggctgtc
    57121 attttataaa ataaggtagt ctcatgcttc ttctgtgaag catgagtaac cagatgttat
    57181 tgcaggccat tattcatttt tcgctaccag caaagagaga tcctgcttcg ccagcgcgcg
    57241 actggcactc tccggcaaac cgtcgtctgt aataatctga tcaaactcgc ttaatggtaa
    57301 cgccagccat gtcgccacct gaccatattt cgtcgcatca cagaccaaaa ctcgctggcg
    57361 gctggcactg gcaatcgccc gtttcaccgt gactttatct tccgctggcg tagaaatccc
    57421 ccgcacactc catgacgatg cagaaataaa agcctgatca atcatcaggc tgcgcagcat
    57481 ggtcgcagcg gcttccccga cacaggaacg gttttcccga cacactgcac cgccagtgtg
    57541 aataattgtg caattactgt tgtcgagcaa gtagttcgca ataacgaaat cgtttgtgac
    57601 cacagtcagt gactccatgt gaatcagatg ctgtgctatc gctaacgtgg tcgttcccgc
    57661 atccagatag atacaacttc ccggctgaac aagacttgcc gccagcttgc caatagccgc
    57721 tttttgcgtc attgccagcg cagtttttac ctgatgagaa ggttcatgcg ccacgcgtcc
    57781 cggagactgg acgcctccgg acaccagcac aacggctccc tgctgctcca gtttttgtaa
    57841 atcccgacga atggtcatat gtgacacatt cattctgtcc gttagttcag caatactgac
    57901 aatgcctttt tcagctacca tctcaaggat gatttggcga cgctctacgg gtatcaactt
    57961 ttgctccttc ctttgtcctg ctgacattct acgctatttg cctgcgaaac gtgcgcggcg
    58021 caactaacgc ttagttcaca taaaataaca cacaatgtta atttatgtga atcagctcac
    58081 catatcgtta tcttccagcg cttatattca caatatcaaa caaaatatca cttaaattaa
    58141 caaggagagc agatgaaaac gggatctgag tttcatgtcg gtatcgttgg cttagggtca
    58201 atgggaatgg gagcagcact gtcatgtgtc cgcgcaggtc tttctacctg gggcgcagac
    58261 ctgaacagca atgcctgcgc tacgttgaaa gaggcaggtg cttgcggggt ttctgataac
    58321 gccgcgacgt ttgccgaaaa actggacgct ctgctggtgc tggtggtcaa tgcggcccag
    58381 gttaaacagg tgctgtttgg tgaaacaggc gttgcacaac atctgaaacc cggtacggca
    58441 gtaatggttt cttccactat cgctagtgct gatgcgcaag aaattgctac cgctctggct
    58501 ggattcgatc tggaaatgct ggatgcgcca gtttctggtg gtgcagtaaa agccgctaac
    58561 ggtgaaatga ctgtcatggc ctccggtagc gatattgcct ttgaacgact ggcacccgtg
    58621 ctggaagccg ttgccggaaa agtttatcgc ataggtgcag aaccgggact aggttcgacc
    58681 gtaaaaatta ttcaccagtt gttagcgggc gtacatattg ctgccggagc cgaagcgatg
    58741 gcacttgcag cccgtgcggg gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc
    58801 gccggaaatt cctggatgtt cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc
    58861 ccgcattcag ccgtcgatat ttttgttaag gatcttggtc tggttgccga tacagccaaa
    58921 gccctgcact tcccgctgcc attggcctca acagcattga atatgttcac cagcgccagt
    58981 aacgcaggtt acgggaaaga agacgatagc gcagttatca agattttctc tggcatcact
    59041 ctaccgggag cgaaatcatg atcaagattg gcgttatcgc cgatgatttt accggcgcga
    59101 cggatatcgc cagttttctg gtggaaaacg gtctaccaac ggtacaaatt aacggtgttc
    59161 caacaggtaa aatgccggaa gcaatcgacg cactggtgat cagcctgaaa acgcgctcct
    59221 gtccggtggt tgaagccaca cagcaatcgc tggcggctct gagctggttg caacagcaag
    59281 gttgcaaaca gatctatttc aaatactgct ctactttcga cagtacggcg aaaggtaata
    59341 tcggcccggt taccgatgca ttaatggatg ctctcgacac gccgtttacg gtcttctctc
    59401 cggccctgcc ggtcaacgga cgtacggttt atcaggggta tttgttcgta atgaatcaac
    59461 tgctggccga atccgggatg cgccatcacc cggtaaatcc catgaccgac agctatcttc
    59521 cccgtctggt tgaagcgcaa tccacagggc gctgcggcgt cgtttcggca catgttttcg
    59581 aacaaggtgt ggatgccgtt cgtcaagagc tggctcgctt acagcaagag ggctaccgct
    59641 acgcggtgct tgatgcgctg accgaacacc atctggaaat tcagggagaa gccttgcgcg
    59701 atgccccact ggtaacgggc ggttctggtc tggcgattgg cctggcccgg cagtggacgc
    59761 aagaaaacgg taaccaggct cgcaaagcag ggcatccgct cgctgggcgc ggcgtagtgc
    59821 tctccggttc atgctctcaa atgaccaacc gccaggtagc acattaccgt caaattgcac
    59881 cagcccgtga agttgatgtg gcacgctgcc tctcaactga aactctggct acttatgcac
    59941 acgaactggc agagtgggtt ctgggccagg aaagtgtact tgctccactg gtttttgcca
    60001 ccgccagcac tgacgcattg gcagcaattc aacagcaata cggtgcacaa aaagccagtc
    60061 aggcagtaga aacactgttt tctcaactag cggcgcggtt agcggcggaa ggcgtggcac
    60121 gctttattgt cgcaggcggt gagacctccg gcgtagtcac acagagcctg ggcataaaag
    60181 ggtttcatat tggcccaacc atttcccccg gcgtgccgtg ggtaaacgca ctggataagc
    60241 ctgtctcact cgcccttaaa tctggcaact tcggtgatga agcctttttt tcacgagccc
    60301 aaagagagtt tttatcatga gcgatttcgc aaaagtagag cagtctttgc gagaggagat
    60361 gacgcggatt gccagttcat tctttcagcg cggctatgca accggttcgg ctggcaatct
    60421 gtcgctgctt ttacctgacg ggaatttact ggcgacaccg acaggttcat gcctgggcaa
    60481 tctcgatccg cagcggcttt ccaaagtcgc cgcggatggc gaatggttaa gtggtgacaa
    60541 accctcgaaa gaggtgctct ttcatctggc gctgtatcgc aacaatccgc gctgtaaagc
    60601 ggtggtgcat ttgcacagca catggtcgac ggcgctttcc tgcctgcaag ggctggacag
    60661 cagcaacgtt attcgtccgt tcacaccata cgtggtgatg cggatgggaa atgtcccgct
    60721 ggtgccttat taccgaccgg gcgataaacg catcgcacag gatctggcgg aactggcagc
    60781 agacaatcag gcttttttac tggcaaatca tggcccagtg gtttgcggtg aaagcctgca
    60841 agaagccgcc aacaatatgg aagagctgga ggaaacggca aagctgattt ttattctcgg
    60901 tgaccgcccg atccgttatc tgaccgcagg tgaaattgcg gaattaagga gttaatgcaa
    60961 tgcctcgttt tgcagctaat ttatccatga tgttcaccga agtgcctttt attgaacgct
    61021 tcgccgcagc gcgaaaagcc gggttcgatg ctgtggaatt tctgtttccc tatgactact
    61081 ccaccctgca aatccaaaag caactggagc aaaaccatct gacactggcg ctgttcaata
    61141 ccgctcccgg agatattaat gcgggggaat ggggattatc cgcccttccc ggacgtgagc
    61201 acgaagcaca cgcagacatc gacctggcac tggaatatgc attggcgctc aattgtgaac
    61261 aagtccatgt gatggcaggc gtcgtgcccg ctggcgaaga cgctgaacgg taccgggcag
    61321 tatttatcga taatctccgc tacgctgccg accgctttgc gctacacggc aagcgaatat
    61381 tagttgaagc actcagcccc gacgttaagc ctcattatct cttctccagc cagtatcagg
    61441 cactggctat tgttgaagag gttgcgcgag ataacgtgtt tattcaacta gatacttttc
    61501 atgcgcaaaa agtagatggc aacctgacgc atttaatccg cgactacgct gggaaatatg
    61561 cgcacgtaca aattgccgga ctacctgatc gacatgaacc ggacgatgga gaaatcaact
    61621 atccgtggct gttccgcctg ttcgatgagg tgggatatca gggctggatc ggttgtgaat
    61681 ataaacctcg tggcctcacc gaagaagggc ttggctggtt tgacgcctgg cgctaattcg
    61741 ttaaatccct aattacaacg tacccataca tccccccatc tacggatggg gtaggttaac
    61801 tataactttc agacagggtt tcccatgtcc acaattacat tgttatgcat tgcgttagct
    61861 ggcgtaatca tgctgttgct gctggtcatc aaggcaaagg tacaaccatt cgttgctctg
    61921 ctcctcgtca gcctgttagt cgcacttgcg gcgggtatac cggcgggcga agtgggtaaa
    61981 gtgatgatcg ccgggatggg cggtgtgctt ggttccgtca ctattattat tggtctgggc
    62041 gctatgctgg ggcgtatgat cgaacactct ggtggtgcag agtcactggc taattatttc
    62101 agtcgcaagt taggtgacaa acgaactatc gctgcgctaa ctctggcagc gttcttcctc
    62161 ggtattcccg tcttctttga tgtcggcttt attattcttg cgccaatcat ttacggtttt
    62221 gccaaggttg ccaaaatatc gccactcaaa tttggcctgc ctgtcgctgg aatcatgcta
    62281 actgttcatg tggcggtacc gccgcatcca ggccctgtcg ccgcagcggg gttactccac
    62341 gcagacatcg gctggctaac catcatcggt attgcgattt ctattcccgt agggattgtt
    62401 ggctactttg cagcgaaaat aatcaatacg cgccaatatg cgatgtcggt ggaagtactg
    62461 gaacagatgc aactggctcc ggccagtgag gaaggcgcaa caaaattaag cgataaaata
    62521 aatccaccgg gcgtcgcgct ggtcacctcg ctaattgtta ttcctatcgc gattatcatg
    62581 gcgggtacgg tttccgcaac actgatgccg ccttcgcatc ccctgcttgg tacgctacag
    62641 ctgatcggct caccaatggt agctctaatg attgcgctgg tgctggcatt ctggttattg
    62701 gctttacgtc gcggctggag cttacaacac accagcgaca ttatgggctc agcccttccc
    62761 actgcggcgg tagtgatttt ggttactggt gctggagggg tatttggcaa agtgctggtg
    62821 gaatcgggcg tcggcaaagc ccttgccaat atgctacaaa tgattgacct gcctctgtta
    62881 ccagccgcat ttattatttc actggctctg cgtgcatcgc aggggtcagc aaccgtagca
    62941 atcctgacaa ccggcgggtt actctcagaa gcggtgatgg gattgaatcc gattcagtgc
    63001 gtattggtga cgctggcagc ctgctttggt gggcttggcg cgtcacatat taatgactca
    63061 gggttctgga ttgtgaccaa atatctgggg ttgtcggtag cagacggtct gaaaacctgg
    63121 acagtgttaa cgaccattct cggttttacc ggattcttaa ttacctggtg cgtatgggcg
    63181 gtaatttgac cttaataaaa aggtccgatg ggcatcggac cttttattta acgcttacct
    63241 tccaccagca gcggcgggat gctcggcacc attggcgcgt catcaatatc tttctgcgtc
    63301 atgcggaacg cttcgggata atgttcgcgg ctggtacggc gcagcggttc ggtatcgcgc
    63361 caggtataaa ggcaatgctg gcactgatat accgtccaga catctttcac cggcgatttc
    63421 gccatcactt caatctgttc atcggcacaa cgtggacaaa tcatcttctt ctccttattt
    63481 acgtgcagcc agcatagcgg tcagtttttc agcccaggct ttggtttccg gtaaatccac
    63541 caccggttgg ctgtagtgac cacggttgtc cggggcgaca ggcgtagtgg cgtcgataat
    63601 cagcttgtcg gtgatccccg cagggcttga gcctggatcg agttccagca cggacatatt
    63661 cggcaactgc accaaatccc ctgccgggtt cactttcgag gagagcgccc acatcacctg
    63721 cggcaggttg aacgggtcaa cgtcttcatc gaccataatc accatcttca cgtagcccag
    63781 accatgcggc gtggtcattg cgcgcaggcc caccgcgcgg gcaaagccgc cgtagcgttt
    63841 tttggtggag ataatcgcca gcaggccatg ggtgtacatg gcgtttaccg cctgcacttc
    63901 cgggaactcg gctttcagct gctgatacag cggcacgcag atggctggcc ccatcaggta
    63961 gtcgatttcg gtccacggca taccgaggta cagcgattcg aaaatcggcc tggtgcggta
    64021 agagacttta tcgatgcgca ccacggtcat gttacgcccg ccggagtagt gcccggtaaa
    64081 ctcaccgaac gccccttcga tttcgcgttt acggctttcg atgacccctt cgaggatcac
    64141 ttctgaaccc cacggcacat caaaaccggt caacggggcg gtggcgatcg ggtacgggct
    64201 ttcacgcagc gcgcctgcca tttcgtactc ggactgatca tatttcagcg gcgtggcccc
    64261 catcagcgtg atgatcggat cgttaccgag cgtgatcgca atcggcagat cttcaccgcg
    64321 ctcttctgct ttatgcagat gcagggcgat atcgtgcatc ggcaccggtt gcaggccgag
    64381 cttacgcttg cccttcactt ccatgcggta gatgccgacg ttctgcttgc cgaagttatc
    64441 cgggtcgagc ggatcgcggg aaaccacgca cgctttgtcg agatagaaac cgccatcgcc
    64501 atcgtttaaa cgaaacagcg gcaggatatc gaacaggttg atctcgtcgc catcaacggt
    64561 gttctgcgcc caggctggat tggcgcggcg ctccggggca atcgggaagt tatcccagcg
    64621 gcggataaac tcatcaatct gctttttaac cggggcattt ggcggcaggc cgagggaaat
    64681 cgcgtggttc tgccaggaac cgatggtgtt catcgccacg cgggcatcgg taaagccgcg
    64741 aatattatca aaccacagcg cgggcgcgcc gtcgccgata cgcccggtgg cgttagctgc
    64801 tgctgccaga tccggctcgg cgttcacttc ttcgctgatt ttcagtaact ggccgtggtc
    64861 atcaagctcc tgtaaaaagc tgcgtaaatc atcaaatgcc attattcatt ctcctgagaa
    64921 aaattccggg cctgcggcaa tccttgccag cgcctggcgt ggggatgttc gaggccaaat
    64981 tgatccagca cgcgggctac cacatggtgg acaatgtcat ctaccgtttc gggatggtta
    65041 taaaaggcag gcatcggcgg caccatcgcc acgcccatgc gtgaaagtgc gagcatattt
    65101 tcgagatgga tggtgctaag cggcatttca cgcggcacca gcaccagttt gcggccttct
    65161 ttgagcacga cgtccgccgc gcgccctacc aggccatcgg cgtaaccagc gcggataccg
    65221 gcgagcgttt tcatactgca cggaataacg atcatgccgt cggtacgaaa agaaccggat
    65281 gagatgatcg ccgcctgatc cgccgggtta tggctgaagt ctgcgagggc agcaacatcg
    65341 cgagcgctgt aaggcgtttc cagttcaatg gtggttttcg cccacttcga catcaccaga
    65401 tgagtctcga cattcggcat ctcccgcagc gcttgcagta atgccacacc aagaggcgca
    65461 ccggtagccc ctgtcatccc gacgatcagt ttcattgctt attctcttaa ttgttcgtat
    65521 acgaacgtta ttattaacat actccttcat atccacagcg gcaaggattg aaaagtaaca
    65581 acatcttctt cgtcactaaa aaaccgctat gatagcgaca gagaattagc ccggagttta
    65641 catggcgtta cgaaataaag cgttccatca gttacgacag ctttttcagc agcacacggc
    65701 tcgctggcag cacgagttac ctgacctgac caaaccacag tacgcggtga tgcgcgccat
    65761 tgctgataaa cctggtatcg aacaggtggc actgatagaa gcagcagtca gcaccaaagc
    65821 aacgctagca gaaatgttgg caagaatgga aaatcgtggc ttagtcagac gtgaacatga
    65881 ctccgctgac aagcggcgac gctttgtctg gctgactgcc gaaggagaaa aggtacttgc
    65941 tgcggcgata cctattggtg acagcgtgga tgaggaattt ttggggcgtt tgagtggaga
    66001 agagcaggag ctgtttatgc agctggtgcg caagatgatg aacgcatagg gggcaaattt
    66061 ccggatgcga cgctatcgcg tcttatccag actacaaata caccgactca ccaaccgaaa
    66121 gaaaaaaggc cagcctcgct tgagactggc ctttctgaca gatgcttact tactcgcgga
    66181 acagcgcttc gatattcagc ccctgcgttt gtaggatttc gcgcaaacgg cgcaggcctt
    66241 caacctgaat ctggcgaaca cgttcacggg tgaggccaat ttcacgacct acatcttcca
    66301 gtgttgccgc ttcgtacccc agcaaaccga atcgacgtgc cagcacttca cgctgtttgg
    66361 cgttcagctc gaacagccat ttgacgatgc tctgcttcat atcatcatct tgcgtggtat
    66421 cttccggacc gttctctttt tcatcggcca ggatgtccag caacgctttt tcggaatcac
    66481 cacccagcgg ggtgtctacc gaggtaatgc gctcgttaag acgaagcata cggctgacgt
    66541 catcaactgg cttatccagt tgctctgcga tctcttccgc acttggttca tggtccagct
    66601 tatgggacaa ctcacgtgcg gttcgcaggt aaacgttcag ctcctttacg atgtgaatcg
    66661 gcaaacgaat agtacgggtt tggttcataa tcgcccgttc aatcgtctgg cgaatccacc
    66721 aggttgcgta tgtcgagaag cggaaaccac gttccgggtc aaacttctct accgcgcgga
    66781 tcagccccag gttgccctct tcgataaggt ccagcaacgc cagaccacga ttgccataac
    66841 ggcgggcaat ttttaccacc agacgcaagt tactctcgat catccggcgg cgagaggcga
    66901 catctccacg cagtgcgcga cgcgcaaaat aaacttcttc ttcggccgtt aacagtggtg
    66961 aataaccaat ctcaccaagg taaagctgag tcgcgtccaa cacacgctgt gtggctccct
    67021 gcgataacag ttcctcttcg gccaaatcgt tatcactggg ttcctcttct actaaggcct
    67081 tttcgtcaaa aacctcaact ccgttctcat caaattccgc atcttcattt aaatcatgaa
    67141 ctttcagcgt attctgactc ataaggtggc tcctacccgt gatcccttga cggaacattc
    67201 aagcaaaagc ctggttccgc cgatttatcg ctgcggcaaa taacgcagcg ggtttacgga
    67261 tttccccttg taacgaattt caaaatgcaa gcgtgttgaa ctggttccgg tgctacccat
    67321 ggttgctatt ttttgccccg ccttcacttc ttgttgttcc cggaccagca ttgtgtcgtt
    67381 atgggcgtag gcactcaggt aatcatcatt atgtttgatg ataatcagat taccgtagcc
    67441 gcgcagcgcg ttaccggcat aaacaacgcg gccatctgcg gtcgcgataa ttgcctgtcc
    67501 tttgctgcct gcgatatcaa tccccttgtt gcccccctca gaagcgccaa aggtttcgat
    67561 cactttgccc tcagtcggcc agcgccaggt ggagataggc gtactggttg atgtactgct
    67621 gacagttggc tcggttgtgc ttgctgttgg taccgttaca ggcgctgtga ccgtggtcgc
    67681 agctggcttg ttgttcggca acattttgtt agcactctgt tcacccgaag attcagaata
    67741 cgtaattgtc ggttgcgacg caacagcaac ggtggaattt tgtgcaggct tgatcacaac
    67801 tccttgctct gctgcgtcgg cctgggtaat ggcatttccg ccagtgattg gcgtaccgga
    67861 agcattaccc acctgcaagg tctgaccaac gttcagcgcg tatggtgcct gaatattgtt
    67921 gcgctgagca aggtcacgga aatcgttgcc agtaatccag gcgatataga aaagtgtgtc
    67981 gccttttttc acggtatagg tactgccgct ataactgcct ttcggaatgt tcccatactg
    68041 acggttatag acgatgcgtc cgttttccat ctgtaccggc tgctgagcta ctggctgcac
    68101 tggctggatt tgcggttgtt gagtagcctg aatttgtggc tgctgtaccg gctgaatttg
    68161 cggttgctgg gctgtagacg tcgtccccat tttcggcggc ggcgtaatca acataccaga
    68221 attggtattt gcaggcgcat tgccattaac ggagctgacc ggtgccggtg gatttgaagt
    68281 gtcagaacag cctgccagcc atagcgaaac cagtgacaaa gccgcaatgc ggcgaacggt
    68341 gaattttggg cttcccgcgc tcatttatcc cccaggaaaa attggttaat aaccagtgac
    68401 ataattaccg tgcaaggcac cctactgaac actggaaaag atgttcacga tacgctgacc
    68461 tgcggcaaaa taaccaggaa aaatccaggt atttcctcac gttttaagcc agctcaccct
    68521 tcactaaagg gacaaagcgc acggcctcca cggtatcgat aataaattcg cctccccgac
    68581 gacgcacccg tttcaaatac tggtgctcct cccctacggg taagacgaga atcccgcctt
    68641 cgtccagctg cgtcattagc gcagttggaa tttccggcgg tgccgccgta acaatgatag
    68701 cgtcaaacgg cgcgcgtgcc tgccaacctt gccatccatc gccatgacgg gttgaaacat
    68761 tatgtaaatc aagatttttc aggcggcgac gcgcctgcca ctgcaaacct ttaatccgtt
    68821 cgaccgagca aacatgctgg acaagatgcg ccaggattgc cgtttgatat cccgaaccgg
    68881 tgccaatttc cagcacccgc gactgcggcg tcagctcgag taattcggtc attcgcgcca
    68941 ccatatacgg ctgcgaaatt gtctgcccct gacctatcgg caaggcgata ttgtcccagg
    69001 ctttttgttc aaacgcttca tcaacgaatt tttcacgcgg cacggcagca agtgcattca
    69061 gcacctgctc atcctgaata ccttgcgcac gtaattgatc cagaagtgct tgtacgcgtc
    69121 tgcttaccat tgcgtgccaa ctcccacgct gtttaaccag tctgaaacca catcttgcgc
    69181 gctatgcgca gttaaatcca catgcagcgg cgtgatggag acatagccct catctaccgc
    69241 agcaaaatcg gtccccggac cggcatcaca tttaccgccc ggcgggccaa tccagtacag
    69301 cgtattgccg cgcggatctt gctgcgggat cacctgatct gccggatgtc gtgtaccgca
    69361 gcgcgtcacg cgaatacctt tgatttggtc cagcggcaaa tccggtacat ttatattaag
    69421 aatacgccct gttcgcagcg gttctttgca cagtgcacgc agaatagaac aggtaactgc
    69481 cgcagcggtg tcgtaatgtt tatgcccgtc tagcgagacg gcaagcgccg gaaaacctaa
    69541 atggcgacct tccatcgcgg cggctaccgt accggaataa ataacatcat cccccagatt
    69601 cggcccggcg ttaattccgg acacaacaat gtccgggcgc ggacgcatca gagcattcac
    69661 gccaagatag acgcaatcgg tcggggttcc catttgcaca gcaatatcac cattttcaaa
    69721 ggtaaacgtg cgcagggagg attccagtgt cagagaattt gaagcgccgc tgcggttacg
    69781 atcgggggcg accacctgaa cgtcagcaaa ctcacgcaag gctttcgcca gcgtttgtat
    69841 accgggtgca tgtaccccgt catcattact cagcaatatg cgcataatca cctgttgtgt
    69901 tgataagttc cctgacaacg ctggttgcaa aactacccgc cggaagccag aaacggatct
    69961 ctacggtgac gtcatcccac caattccagc ttaattgttg cggatacagc agcatcgctc
    70021 tgcgcgcggc ttcaactttt tcgcgcacca gtaaagcttg taattcagtt tctgcggcga
    70081 cagctgcttg ttcgaatgcc agcgcttcac gctgagttcc ccattcgcca ctgcccggca
    70141 atgcggcggt tatcatcagc tctttatcgt tgacgcgacg ctgtaattcc gccagttctt
    70201 cggtggttgc gacaaaccag ctaccacgtc cggctaattg tagcgcatcg ccgtcaacaa
    70261 cttgattaac gtctgctttt ttgaggcgct cagcaacaat ctgattaaac aacgcactgc
    70321 gggctgccga caaccaaaaa ctccgtttat tgcgatcgcg caccggagta ttggtttgcg
    70381 cccagcgctg cgccccctgc aagttgctac cgccaatccc aaaacgttgg gcaccgaagt
    70441 agttcggtac accttttacg caaatatcga tcagacgttg ttcaacgtca tcgcgattgc
    70501 tcacttcgcg cagaaccagg gtaaaggcgt tacctttcag cgcgcctaaa cgcagcttgc
    70561 gcttgtgccg cgcatactcc agcacctggc agccttccag ttgaaaggcg ctcagatcgg
    70621 gcatttcctt gcctggcacg cgagcgcata accactgttc cgtaacagca tgtttgtctt
    70681 tttgcccagc gaagctgact tcacgggcat gaattttcag gaatttcgcc agtgcatccg
    70741 ccacaaaacg ggtattgcag ccgtttttga ggattctaac cagaatatgc tcaccttcac
    70801 catcaggctc aaagcccaaa tcttccacca ccacaaagtc ttccggattg gctttcagca
    70861 gcccggtgcc ttgcggttta ccgtggaggt aagtgagatt atcaaactca atcattttgt
    70921 tgccttaatg agtagcgcca ccgcttcaca ggcaatccct tccccacgtc cggtaaatcc
    70981 aagtttttcc gtagtagtgg ctttcacgtt aacatcatcc atatggcagc cgagatcttc
    71041 ggcaataaac acgcgcattt gtggaatgtg cggcaacatc ttcggtgcct gagcgatgat
    71101 agtgacatcg acgttgccca gggtataacc cttcgcctga atacgacgcc aggcttcgcg
    71161 tagcagctcg cggctgtcgg cacctttaaa tgccggatcg gtatccggga acagcttgcc
    71221 gatatccccc agcgccgccg cgccaagcaa tgcatcggtc aacgcatgga gcgtcacgtc
    71281 gccatcagaa tgcgccagca atcctttttc gtaaggaatg cgtacgccac caatgataat
    71341 tgggccttca ccgccaaagg catgtacgtc aaaaccgtgt ccaattcgca ttatgtattc
    71401 tcctgatgga tggttcgggt gaggtaaaac tcggccagtg ccaaatcttc cgggcgcgtg
    71461 actttaatgt tatccgcacg gccttcgacc aactgaggat ggaatccgca atattccagc
    71521 gccgaggctt cgtcggtaat agtcgcgcct tcatttaaag cgcgcgtcag acagtcatgt
    71581 aacagctcac gagggaaaaa ttgcggcgtc agcgcgtgcc ataagccgtt gcgatcaacg
    71641 gtatgagcaa tggcattttt gcccggttcg gcacgtttca tcgtatcgcg cactggtgcg
    71701 gctaggatcc ctcccgtgcg gctggtttcg ctcaacgcca acaatcgcgc gaggtcatcc
    71761 tgatgcagac aaggacgagc ggcgtcatgc accaataccc actgcgcgtc gccagcggct
    71821 ttcaaacctg ccagcacgga atcggcacgc tcatcaccgc catctacaac ggtgatttgc
    71881 ggatgattcg ccagaggaag ttgtgcaaaa cggctatcgc caggacttat ggcaatgacg
    71941 acacgtttca cccggggatg cgccagcagc gcatgcaccg agtgttcaag aatggtttga
    72001 ttaccgattg agagatattg cttaggacat tccgtttgca ttcgacggcc aaatccggcc
    72061 gccggaacca cggcgcaaac atccaaatga gtggttgcca tgttaattcc cgggctgatt
    72121 tatcgattgt tttgccccgc agactgtgcg cgcttcgacg cgtcaggcac cagacgataa
    72181 aaagtttcgc ccggcctggt catgctgagt tcattacgcg cacgctcttc gagcgcctcc
    72241 tggccgccat tgagatcgtc aatttcggca aaaagttgat cgtttcgcgc tttaagtttc
    72301 gcgtttgtag cttgctgtgc cgccacatca tcattgacgc gggtatagtc atgtataccg
    72361 ttcttaccga accacagcga atactgtagc cagaccagaa tagccagcaa cagcagcgtt
    72421 agtttaccca tcctgccccc tgaaaaacgg catcatcatc ccatgcatcc gaagacgact
    72481 ctacatcctc tgttggggat accgcgacaa cgcgggcaaa tgtaccacat ttgtccattg
    72541 ttacgtatac ccagggcgtg cagaacataa tctcattatt agttacggtt tgaattatga
    72601 acagaggaga caagaaagta caaattagcc cagtagccac ataaacagtg cgccaaacat
    72661 aatgcctact gtcaccaggg tgaaaacaat actgtagcgt agctttccgt ccatcaatga
    72721 atgcagcgca atccccacca ctaccgcgac gggcatcagc gccagaaaga aaggccaggt
    72781 gtagataaag aagaacagcg tgttagagcc ataaatcaac atcggcatcg ccagcgcaaa
    72841 taaccaggag ataaaaccga ccacggcacc aggcagtgac catgtggttt cttcatcctc
    72901 agtaaggctg tcgttatttg ttagtgtaat gttatggcta ttacgcatat ttgatcctgt
    72961 tactttgacg aaccgggcat ggaaacccgg tggtgtctca ggatctgata atatcgttct
    73021 gtctcaacag atctaataat tgctgtacca aatttgttac taattgttca ccattgagat
    73081 gaatttctgc cgattcaggc gcttcgtaaa cggaatctat tcccgtaaag ttgcgcagtt
    73141 caccggcacg cgctttctta tataagcctt tgggatcgcg ggcttcgcaa atcgccagcg
    73201 gcgtatcgac aaacacttcg ataaagcgcc cttctcctac gcgttcgcga accatctggc
    73261 gttcggcgcg gtgtggcgag ataaatgcgg tcagcaccac cagtccggct tcaaccatca
    73321 aattcgccac ttcaccgacg cgacggatat tctctttacg atcggcatcg ctaaaaccga
    73381 gatcgctgca taatccgtgg cgaacattgt cgccatccag cagatacgta ctgacgccga
    73441 gtttatgtaa cgcctcctcc agcgccccgg cgaccgttga tttaccggac ccagagaggc
    73501 cggtaaacca cagcactaca ccacgatgac cgtggtgtag ctcgcgttgt tgcacagtga
    73561 ccggatggct atgccagacg acgttttcgt catgcagcgc cattatttat cccccagcaa
    73621 atcgcgcgcg ccccagtgcg gaaagtggcg acgaaccagg gcattcaatt ccagttcgaa
    73681 tgcactgaat tcagatgacg caacggcggc ctcgtttttc ggctcatgca ccataccggc
    73741 accaacagtc acgttgctca ggcgatcgat aaaaatcagc ccacccgtca ccggattttg
    73801 ttgataacga tctaacacca gcggctcgtc aaaagtgaga tccacgaggc cgatcccatt
    73861 cagtggcagg ttttcaactt cacgctgggt aaggttatta atatcaacct gatagcgaat
    73921 gccatcaata cgcgcgcgcg tcttcttacc ggcaattttg atgtcgtaac tctggcccgg
    73981 ggaaagcggc tgttccgcca tccataccac atccaccgac gcgctctgca ccgccggtaa
    74041 cgcttcgtct gccgccagca gcagatcgcc acggctgatg tcgatctcat ccgtcagcac
    74101 cagggtgatc gcttctccgg caaaggcttc ttcgcgatca ccatcaaaag tcacgatccg
    74161 cgcgacgttt gattccacac cagagggcag cactttgaca cgttgcccga cttccacgcg
    74221 accggatgcc agcgttccgg cgtagccacg aaaatcgaga ttcgggcgat taacgtactg
    74281 caccgggaag cgcattggct gagcatccac cactcgctgg atctccacgg tttccagcac
    74341 ttcgagcagt gtcggaccgc tgtaccacgg catactttca ctttgcgaag ccacgttgtc
    74401 gccttccagc gcagagagcg gcacaaagcg aatatccaga ttgccaggta actgttcggc
    74461 gaaagtcagg tagtcttcac gaatgcgggt gaatgtctct tcactgtaat ccaccagatc
    74521 cattttgttg atcgctacga ccagatgttt gatccccagc agcgtggaga taaaactgtg
    74581 acgacgggtt tgatcgagca cgcctttacg ggcatcgatc agtaagatcg ccagctcaca
    74641 cgttgaggcg ccggtcgcca tattgcgggt atactgctcg tgcccggggg tgtcggcgat
    74701 aataaattta cgcttctcgg tagagaaata gcggtaggcc acatcaatag tgatgccctg
    74761 ttcgcgctca gcttgcaggc cgtccaccag cagagccaga tccagttttt cgccctgggt
    74821 gccgtgacgc ttactgtcgt tatgcagcga tgagagctga tcttcgtaga tttggcgggt
    74881 atcgtgcagc aggcgaccaa tcagggtact tttgccgtca tcgacgctac cacaggtcag
    74941 aaaacgcagc aggcttttat gttgttgcgc aatcatccag gcttcgacgc cgccttcatt
    75001 agcgatttgt tgtgcaagtg cggtgttcat cttaaaaata cccctgacgt tttttcagct
    75061 ccatagaccc cgcctggtcg cggtcaatca cgcggccctg acgttcactg gtggtggaaa
    75121 ccagcatctc ttcgatgatc tccggcagtg tttgtgcatt tgactccacc gcaccggtca
    75181 gcggccagca gcccagcgta cggaaacgca ccatccgttt tttaatcact tcgcccggtt
    75241 gcaggtcgat acggttgtca tcaatcatca tcaacatacc gtcgcgttcc agaaccggac
    75301 gttccgcagc gagatacagc ggcacaatgt cgatattttc cagccagatg tattgccaga
    75361 tatcctgctc ggtccagtta gagagcggga agacgcggat gctttcgcct ttgttaattt
    75421 gcccgttgta gttgtgccac agctccgggc gctgattttt cggatcccag cgatggaagc
    75481 ggtcacggaa agagtagatt cgctctttag cgcgggattt ctcttcgtca cggcgcgcac
    75541 caccgaaggc ggcatcaaaa ccgtatttgt tcagcgcctg tttcaggcct tcagttttca
    75601 taatatcggt atgtttcgcg ctgccgtgca cgaatggatt aatccccatc gccacgcctt
    75661 ccaggttttt atgcaccagc agctcgcagc cgtaggcttt cgccgtacga tcgcggaact
    75721 catacatctc gcggaatttc cagccggtat cgacatgcag caacgggaaa ggcagcgtac
    75781 ctggataaaa cgccttgcgc gccagatgca gcatgacgct ggaatcttta ccgatagagt
    75841 agagcatcac cggatttgag aattctgccg ccacctcgcg aataatgtgg atgctttccg
    75901 cctccagttg ccgcaggtga gtaagtcgta tttgatccat aaccgttcct ttgcaatacc
    75961 gctattttct tgccatcaga tgtttcgact atagggagcg taagagaacg aatgaaatta
    76021 ccaattagaa tgagtagttc cttaacggaa taacgatttg gcaaagctaa tatcaaaaag
    76081 tgcttaaggc accggatttc gggcgtttag gaagatttga aattgtttta gcgcagcggc
    76141 agtttcatac tatggcggta aaaaaatttg catggtattt aaggactcac tatgttttcc
    76201 gcattgcgcc accgtaccgc tgccctggcg ctcggcgtat gctttattct ccccgtacac
    76261 gcctcgtcac ctaaacctgg cgattttgct aatacccagg cacgacatat tgctactttc
    76321 tttccgggac gcatgaccgg aactccagca gaaatgttat ctgccgatta tattcgccaa
    76381 cagtttcagc aaatgggtta tcgcagtgat attcggacat ttaatagtcg gtatatttat
    76441 accgcccgcg ataatcgcaa gaactggcat aacgtgacag gaagtacggt gattgccgct
    76501 catgaaggca aagcgccgca gcagatcatc attatggcgc atctggatac ttacgccccg
    76561 ctgagcgatg ctgacgccga tgccaatctc ggcgggctga cgttacaagg aatggatgat
    76621 aacgccgcag gtttaggtgt catgctggaa ttggcagaac gcctgaaaaa tacgcctacc
    76681 gagtatggta ttcgatttgt ggcgaccagc ggcgaagagg aagggaaatt aggcgctgag
    76741 aatttactca agcggatgag tgacaccgaa aagaaaaata cgctgctggt gattaatctc
    76801 gataacttaa ttgttggcga taaattgtat ttcaacagcg gtgtaaaaac tcctgaagca
    76861 gtaaggaaat taacgcgcga cagggcgctg gcaattgcgc gcagtcacgg aatagccgca
    76921 acgaccaatc cgggtttgaa taaaaattat ccgaaaggca ctgggtgttg taatgacgca
    76981 gaaatattcg acaaagcggg cattgctgta ctttcggtgg aagcgactaa ctggaatctt
    77041 gggaataagg atggttatca gcaacgcgca aaaacagccg cattccctgc gggaaatagc
    77101 tggcatgacg taagactgga taatcaacag catattgata aggcacttcc tggaagaata
    77161 gaacgtcgct gccgtgacgt tatgcggata atgctacctc tggtgaagga gttggcgaag
    77221 gcgtcttgat gggtttgaaa atgggagctg ggtgttctac cgcaggggcg gggaattcta
    77281 agtgatatcc atcatcgcat ccagtgcgcc cggtttatcc ccgctggcgc ggggaactct
    77341 tactgcttgg tatgcggaat cacaccctga acggtttatc cccgctggcg cggggaacac
    77401 tgaagcatca aacatttggt ggaccaaacg gacggtttat ccccgctggc gcggggaaca
    77461 ctgtacgcgg cgagttttag cgacaggtca tcccggttta tccccgctgg cgcggggaac
    77521 acggatctgc cagcgcctct gcggggcggt aaaccggttt atccccgctg gcgcggggaa
    77581 cacactaagc atacataact gtttttaaac aaatttattc cgcatcaaca atctaccaac
    77641 taaattaaaa catttcctta tttttaaaga acgtctaact tattgattat caacaggaag
    77701 aaaagaaacc aaacgtaacc catccaaatc caccggaata cgtctgtttt ctccccaggt
    77761 ctgaaattca aaacccgact cggtattggt cgcccaggcc atcaccacat ttccgcaacc
    77821 agccagttgg gtaatttgct gccagatcat ctcccgaata cgttttgatg tatcaccaac
    77881 atacacaccg gcacgcactt ccagtagcca gattgcgagc cgtccacgta agcgcggagg
    77941 gacattttct gtaacaacca cgaccatgct catccgccgc gcccccggtg accactatca
    78001 cccagcgttt caggttcagg gatggcaggc ggtaacatat ccggcgcggg ttgtggtggt
    78061 tcaatttcac ctgcagcaag gacttcctca attaacggta ttaatttgcc cgttaactta
    78121 gtgctacgga aaatatcgcg acaggctaat ctgacttctt tatcaggttc tgcgggttgc
    78181 ctcgctgcta tttcaaatgc ctttggcaca accgaatcaa atttaatgat atcggctatg
    78241 tcataaacaa atgaaagcgg tttgccacta tggataaacc caatagcggg cgcatatccc
    78301 gcggctaata ctgccgcttc agatataccg tacagacatg atgtagcagc actgatgcag
    78361 cgattcacaa catcgccttt ttcccagtct ttaggatcgt atttgcgacc attccatttc
    78421 acaccatatt gtttcgccag taatgcatac gtctggcgaa cgcgagatcc ctcaattcca
    78481 cgtaactgct ccactgaacg gcgagctggc tgtggttcac gaaaacgtaa ttcatacatt
    78541 ttgcgcacca ccttcaaccg tagatcttcc gttaaagcca gctttgcctg gtagagtaat
    78601 ttatctgccc gcgccccacc gggttgtccg gaggagtaaa cgcgaacgcc cgcttcaccg
    78661 acccagacca gcagtgttcc caccgtggcg gccagatgca ccgccgcgtg ggaaactctc
    78721 gttcctggtt cgagcataat gcaggcgacc gatcccaccg gaatatgcgt tcggatccct
    78781 gttttgtcga tcagcacgaa agcgccgtcc agtacgtcga tctgaccgta ctggaggaag
    78841 atcatggagg tgcgatcttt taatgggatc gggctcagtg gtacaaacgt cacgcctctg
    78901 ctccgggttt gatcagcatc agaccacagc cgaacgcccg gcttttgcca tacccctgac
    78961 tgagacgttg cagaaataac ccgggatcgg tgaccgtaag catccccgta taatccacgc
    79021 tactgaactg gatcagttgc cgggtgtttt cccgccggag ttgttgttgt cggtaggcat
    79081 caaccgaggt atccagtagt gtaaaaccac ttctttcccc ctgcgctgcc agccagtcca
    79141 gcgccgcctg ttgttgatgc aaccagatat cacgcccttc cgcctgcccc ttcacctgcc
    79201 gtttcgcctc catcagcaga tcgtggcgct tgcccgcttt acagattgtt ggattcgcct
    79261 gcaggttgaa acaaagagat tgtccggtac gcagctcgga cacaaatggt cggcattcga
    79321 tggtgaaagt ttcactttcc gccgggcgtt cctgcgagag gacaaaaaag cgaaacgcgc
    79381 cctggagttc ttcccgacga taaagaaatt gcctttcttt gccgccaggg aagagatccc
    79441 acagccactg atgcatcaca tattcaccgc gatccaccag atgcagcaac tgcgctgggg
    79501 aaagctggcc ggtatgtaag gttattcttg agaggtacat ggttcctcct tgctgagcca
    79561 cggcccctga ttgacggtgc gttccccaaa caaccactgc tgacgattta aaggaacatc
    79621 ccgacggcgt aatattttgc tcgcgaccag gccgtcgtgt tccccttccc accagcattc
    79681 atcctgaagt tttgggagtg agactttcag tttgcggaaa tgatcctgat actgttggta
    79741 tgcgttacgt aaggtatccg acgcgttgcc ttcgagtagt aacggcgcaa gtggtaacgc
    79801 cagcggatga ctttttcgcc ccagataaag cggaaaaacc ggatggcgta aaccgtcctg
    79861 caactgttcg aggctgtaag gcgcatcggg ggttgttgcc accgccacca tccaccaggc
    79921 atcggtgtag tagtcgcgcc gggagataat cgcgctcaaa agctcagggg cgctcaactc
    79981 ttcgcgacgg ctgaaataac gtgctttacg cacctctttt ggcatctgga ccgtgtgata
    80041 atcccgtgcc cagcgcgggt tacggctggc gcaaaccacc agtgaatagt ggcgattaaa
    80101 cgcgtttaag cgttcggtat catcacgccg gatccctacc ccggcagcca gcagccccag
    80161 caatgctgag cgcgaaggca gttcatgggt atgacgcact tcgccggggg catcgacgcc
    80221 ccaggatgcc atcggcccat gaagctgaaa aatcagatat tggctcataa gccgcccctt
    80281 acgcgcagat aaagtccagc acgtccttca tgcttccctg tttgttcatc acgtcaaaac
    80341 ttgcgtaatc ggtcttctgt tcgtagaccg tattcatatt ttcgcgaaga gttgtaatac
    80401 gctgcaccgc cacctctaac tgacgggtgc cattaatggg ttcatagaaa gctgccgcca
    80461 gagaacgcgg ttgatcggtg cctttttctg ccagcgccca ggaggcgtag gcacggctgg
    80521 caaagctgtt ctgtttgccg gttggggaga ctttaagtgc ggcttccgta aaggcacgta
    80581 tggtctgatt agctaacgct tcgtcaccgc caaggttttc gaccagcaga tctttatcaa
    80641 tgcagatata ggtgtagaac agtgcagaac cgaatccggt ttcaccaaga tgcccggcac
    80701 cggcatcttc agaagcctgg cgcaaatcat caacggcggt gaaaaaatca tcttcgacaa
    80761 tcgtttcact gacaccaaat gcgtgcgcta cctggcaggc ggcttcaaca ttaaactcgg
    80821 gtttattcgc cagcatacga ccaaacatgg caatatctac cgccatacgg tctttgcgta
    80881 acaacgcgag atcttcctct tttggcgcgc gcttttcttc tgcaagctga tgggccagcg
    80941 cttttacggc gtcaaattct gccgggctga tatggactaa ctgttcagtt tcggcgttag
    81001 tgagcggatc ttttggtttt ttgtcgtttt tagctttccc aagataatct gcaatttttg
    81061 ccgcccattc gatggctttt ttctcttcga ttcctttctc aatcaggata gttgccgcct
    81121 cacgcgcaat acgcccactg cgaataccaa tatggcccgc cagtgcctgt tcaaaaagtg
    81181 cagaagtgcg ccacgcacgt ttcagacttt gcgaggaaac gcgcagacgc gttgctccac
    81241 ccagcaccac agtttttggc gcaccggtat catcacggtt aaggttggca gcagggtaag
    81301 cggttaacaa atgaagctga ataaatgttg tcatgagata gtcctttatt gattttgttc
    81361 gttatcggcg tcgccagcct ggtaatattc cagcgcccag cggatacgga taaattcggt
    81421 tggccgctgc tggcggcggt gatgattgag caggtcgtcg ctctcctggc accaacggaa
    81481 gaccccctct gccagagagt caagattgac ggaaccgttt aataatctga ctgcgcggcg
    81541 taactggcgc agtaattcat ccggtgtttt tactgccgac agacgagtaa aacgcccctt
    81601 tgacattacc gccgccagct gcgcggcaaa aggctgccgt tcatcaatag ctttaacatt
    81661 ggcgctaact gcggctatca gcgccagtgc ggtgatacgc cactcaggtt catcctgcca
    81721 ctttatttgt ctgttcttca gaaacaggcg aaatccatcc gtcagacaaa catcattaac
    81781 cgtcgtacta cgccgcagac tggcacgttc gccacgtttc tcctgcaatt cctcgtgcca
    81841 tttgcgcagc gtggctttgt gctcctcttt tacaatactc attcagcagc ctcctgcttt
    81901 ttctccctgg cggctttagc actttgcttc tccgccgatg ttgtgaaata tttcttgcgc
    81961 gcggtcataa cgcgtttcaa atcaacaggc tcatatggat tggtgaatac acgctcgtca
    82021 aaatcctgac gcgcgaataa ccaaatttcc ttttgccatt tgccgagtaa ttcgtccgca
    82081 tcctgacctt cttcaatttg gcgcactaac ctcaggaagc gatgctgagt tttgttccag
    82141 aagtcgatat ccacaaaact gaaatcaccc cgtgcacctt ttggatcgga gaaccacgct
    82201 tctttcaatg cacttcgtaa cagacttaga atccgtgaag ccgtttgcgc agccagacgc
    82261 agtttcggta tctggccttc ttttttcttg agcagcagcg ggaaatggtg ttcgtaccaa
    82321 cagcgcgctt tcatgttgtc gaaatcatag ccaaatcccc acaggcctac ttttgcctgt
    82381 ttcagactgc tggcattaaa gagtttcacc accagcgcgg gaagttccgt attgttttct
    82441 gatttacccg tttcgataag gcctaaccag tcgcgccaga ttaaaccgcc cggttgtggt
    82501 ttgaccgagt aaaactcgcc accctcttta agtggtatac ggtaaggcgt taagggatgc
    82561 tgccacatgg cataattcgc accgtaattt ttggtcgtca tcaggctcag gagcgcgtcg
    82621 ctctgttcac cgcaaatatc gcagttgccg actgtcgtgg tattaaaatc aatacgaata
    82681 cggcgcggca ttccccagta agcctggagt ttattgacct gatcatgggt taccaccgca
    82741 ccggccagtt cgctggttcg cgtcgggcca agccagggga aaaccagatc gtcaaatttt
    82801 ttgggtagcg gtaagtcggc ttcatcctgc ggcatcacat tgggccacag tttgcgccac
    82861 aagggcgttt gttgattgcc ttgatattcc tgtaattcaa tcagagtggt catcggccca
    82921 ccgccgcgca aaccggtgcg atagcctttg ccgcctgatg gcgcatttaa ctgtagggag
    82981 aacagagcta acgcggaaca atgagggcat acgtgttcag tcacgccacg cttaataaag
    83041 tggtctttat taaacttcgt tgtttgagcg ccgggaatct caggcagtag cgaagcgacc
    83101 tgaactttat ctcccttgag cgcctcgaaa tcctgcataa atgaagggga atctgcccca
    83161 aactggaaag cgtgttctaa ggacagcaat gcttcccgta gcttttcagc ctccagtccg
    83221 tcttgccaga tatcatccca acgacgatga tcttttggcg caaagctggt ttgtagtagc
    83281 cccagcaaaa actgccatgc cgctccctgg agatctgccc gtggcgcagc aatatcgaca
    83341 acattttcat ccgccagatc gacaggcgcc agtttgcctg tcgttccatc tttgaaacga
    83401 acaggcaacc acggggttgt cagaagtgaa aacgagttca tacgcatcct ctcgtaaaaa
    83461 agaccatccc aggtcggttg tccatcattc catccttttc ggtgtatcaa ctattcagag
    83521 agccctgtcg caggcatctc aaccaacctt gccagtcaat ccttccatag ccgaataccc
    83581 gcaactttcg tcatcggtga ctaaaatcac gtttgccatc tccggatctt gccgctgttc
    83641 aatgcaccac tgcctgaacg catccccttc cagtaaagaa aactcatccc gatgtttttt
    83701 ccaccagctt cggcgcactc tgacaacgct catttcccat gcgtgagcac cggtggcata
    83761 aggcttcacc acaccgtcaa tacaggtagc cagccacagg gaaacagact cctcagccag
    83821 acgtgtcgac agtttttccg ggaggtaatc gttgatattg gcggcatagc cgggcctgaa
    83881 gttcagcaca aactttttag cccttgcgcg atcacagtaa tatttgccca cttgctcctg
    83941 ctcgctgcgg gcaaatcctt ccggcattac cacgtcctca ccgtagacgg attcaataag
    84001 aagccgggct gagtgtggca tctgaatagc accttgctca cgcagcacac gctgcgtcag
    84061 ccagattcgc ccatgatcgg gatagacaaa tgcactgtta cgcatggcac tgccaaacca
    84121 ttcgtcgcca ggagagtcgt cccagacggg agccagaatc agcaattcag ggggggaacg
    84181 ctcgtccttt ccgtcacgct ttaactgacc attaatatcg cggatatgcc gctgtaatcg
    84241 tcctgctcgc tgaatcagca aatcaacagg ggccaggtcg gagatcattt cgtccaggtc
    84301 aatatccacg ctttgctcaa ttacttgtgt acaaacaatg acttgtgacg ctctctgtaa
    84361 actacaatat ttaccaaagc gtgccagtgt ctccgtttca attcgctggc gatcgctaaa
    84421 agcaaagcgg ctatgaaaaa gtgaaaggct ggaagcagga atgacgccgc gggcaagcag
    84481 ctgacgatga accttaatag cgtcatcgac agaattccgg atccaggcga tgcatttccc
    84541 ctgacttacc gccgattcga tacgcgcaat acactcttgt tcactatgaa gccaacccac
    84601 gctgacgcta cgctcaacgt ctttgcgcgt cgctacccgg tgtgagttca catcggattt
    84661 cgtgacatgc gtcagccagg ggtaatcatc cttttccagg aacggagctt cttgcaggcc
    84721 ctctgtaccc cgcgcaaagg cagcgacgag tttgtcgcgc tgctgttgag ataacgtggc
    84781 agaaagcaaa ataacgctgt ttccgccacg cgcctgccgc tcgatcaacc cttcaagaat
    84841 gcacgacatg taagcgtcac aagcgtggat ctcatcagcc agcaggattt tgttactcag
    84901 ccccagaagc cgcagattat tatgtttgaa cggcatcacc gccatcatcg cctgatccag
    84961 cgtgccgacg ccaatttcag ccagcaacgc cttcttgtta ctgtcggcaa accaggccgc
    85021 acatccctga ctgaacgttt gttcatccgg ttcttctgac ccgactaaat caccagacca
    85081 gagtgattca ttaaagcggt ccattaatgt gcgggcacta tgtgccagca ccaggctggg
    85141 gcgggattct ggcgaataga aagcaagcca ggttttgacc agccgatcgt acatggcatt
    85201 ggccgttgcc attgttggca gaccaaaaaa cagcccctga gctttccccg cagctatcaa
    85261 cctgtgcgcc aggataagcg ccgcttctgt tttacctgcg ccagtcacat cttccagaat
    85321 aaataactgc ggtccttgct ggctgatatc cagatccagc accttttgct gtaacggtgt
    85381 cggattctca ataaaaggaa acagcgtatt aattccgttg aaaggcgcga cttttgcttt
    85441 tagaggaaag acggttaagg cgttttgagc ctgaattaaa gttttctgcc agtaaccttt
    85501 aatatccatt gggtgtgcga cgcgtggaaa aaatcgcgtt gacgaacccg tccagtctgc
    85561 gagcacgacg gttgcagaga tataccagga aaggtgtttt ataagttcaa tgccctcatc
    85621 atcatcccag aatgcgggga tctctatgag cggaaacagt ggtttgattt caaggagaaa
    85681 atctcgtgct gcagctttgt cttcaggcag aaaattatcc aattcatcca tacgatcagg
    85741 gggccgacca tgatgccctg tcgtcacggg catccacatt tctatcactc gcctaagttt
    85801 gcgcggagag agcgaagatg aaggaagtaa ctcctgacat tcacttaaat aatgattcca
    85861 caaccagtag cccagcgttg aatgagagat tttttcgtaa ttttttctgg caccttcctg
    85921 aatcttgagt tcagggggca ggtaaagttg ttgaaaagag cgggcaaatt ttccaatatc
    85981 gtgccagcac agcaaccagg cgaaaaattg agccgcctgt tccttgtcag aaatccctaa
    86041 ttgacgaaaa taatcagcca gcccgaagca atttctttta accattaaat agcccatcgc
    86101 ggctacatcc agcgaatgcc agcaaagaag gtgatagtc
//
LOCUS       DAZFRN010000002        72816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFRN010000002 DAZFRN010000000
VERSION     DAZFRN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.002"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..515
                     /locus_tag="ACHKUB_000092"
     CDS             <1..515
                     /locus_tag="ACHKUB_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6653177.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            531..1070
                     /locus_tag="ACHKUB_000093"
     CDS             531..1070
                     /locus_tag="ACHKUB_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO6653178.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSESWFN"
     gene            complement(1163..2740)
                     /gene="guaA"
                     /locus_tag="ACHKUB_000094"
     CDS             complement(1163..2740)
                     /gene="guaA"
                     /locus_tag="ACHKUB_000094"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO6653179.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2809..4275)
                     /gene="guaB"
                     /locus_tag="ACHKUB_000095"
     CDS             complement(2809..4275)
                     /gene="guaB"
                     /locus_tag="ACHKUB_000095"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO6653180.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4437..5807
                     /gene="xseA"
                     /locus_tag="ACHKUB_000096"
     CDS             4437..5807
                     /gene="xseA"
                     /locus_tag="ACHKUB_000096"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO6653181.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5804..6019)
                     /locus_tag="ACHKUB_000097"
     CDS             complement(5804..6019)
                     /locus_tag="ACHKUB_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6653182.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6088..7560)
                     /gene="der"
                     /locus_tag="ACHKUB_000098"
     CDS             complement(6088..7560)
                     /gene="der"
                     /locus_tag="ACHKUB_000098"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO6653183.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7678..8856)
                     /gene="bamB"
                     /locus_tag="ACHKUB_000099"
     CDS             complement(7678..8856)
                     /gene="bamB"
                     /locus_tag="ACHKUB_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO6653184.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8867..9487)
                     /gene="yfgM"
                     /locus_tag="ACHKUB_000100"
     CDS             complement(8867..9487)
                     /gene="yfgM"
                     /locus_tag="ACHKUB_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO6653185.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9505..10779)
                     /gene="hisS"
                     /locus_tag="ACHKUB_000101"
     CDS             complement(9505..10779)
                     /gene="hisS"
                     /locus_tag="ACHKUB_000101"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO6653186.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10890..12008)
                     /gene="ispG"
                     /locus_tag="ACHKUB_000102"
                     /gene_synonym="gcpE"
     CDS             complement(10890..12008)
                     /gene="ispG"
                     /locus_tag="ACHKUB_000102"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO6653187.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12035..13048)
                     /gene="rodZ"
                     /locus_tag="ACHKUB_000103"
     CDS             complement(12035..13048)
                     /gene="rodZ"
                     /locus_tag="ACHKUB_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO6653188.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13333..14487)
                     /locus_tag="ACHKUB_000104"
     CDS             complement(13333..14487)
                     /locus_tag="ACHKUB_000104"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO6653189.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14637..15068)
                     /gene="ndk"
                     /locus_tag="ACHKUB_000105"
     CDS             complement(14637..15068)
                     /gene="ndk"
                     /locus_tag="ACHKUB_000105"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO6653190.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15217..17529)
                     /gene="pbpC"
                     /locus_tag="ACHKUB_000106"
     CDS             complement(15217..17529)
                     /gene="pbpC"
                     /locus_tag="ACHKUB_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO6653191.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17530..22491)
                     /gene="yfhM"
                     /locus_tag="ACHKUB_000107"
     CDS             complement(17530..22491)
                     /gene="yfhM"
                     /locus_tag="ACHKUB_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO6653192.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAGFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22698..23543
                     /gene="sseA"
                     /locus_tag="ACHKUB_000108"
     CDS             22698..23543
                     /gene="sseA"
                     /locus_tag="ACHKUB_000108"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO6653193.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23879..24004
                     /gene="timP"
                     /locus_tag="ACHKUB_000109"
     CDS             23879..24004
                     /gene="timP"
                     /locus_tag="ACHKUB_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO6653194.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24036..24812)
                     /gene="sseB"
                     /locus_tag="ACHKUB_000110"
     CDS             complement(24036..24812)
                     /gene="sseB"
                     /locus_tag="ACHKUB_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO6653195.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24954..26237)
                     /gene="pepB"
                     /locus_tag="ACHKUB_000111"
     CDS             complement(24954..26237)
                     /gene="pepB"
                     /locus_tag="ACHKUB_000111"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO6653196.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGGRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26415..26615)
                     /gene="iscX"
                     /locus_tag="ACHKUB_000112"
     CDS             complement(26415..26615)
                     /gene="iscX"
                     /locus_tag="ACHKUB_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO6653197.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26627..26962)
                     /gene="fdx"
                     /locus_tag="ACHKUB_000113"
     CDS             complement(26627..26962)
                     /gene="fdx"
                     /locus_tag="ACHKUB_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO6653198.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26964..28814)
                     /gene="hscA"
                     /locus_tag="ACHKUB_000114"
     CDS             complement(26964..28814)
                     /gene="hscA"
                     /locus_tag="ACHKUB_000114"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO6653199.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28831..29346)
                     /gene="hscB"
                     /locus_tag="ACHKUB_000115"
     CDS             complement(28831..29346)
                     /gene="hscB"
                     /locus_tag="ACHKUB_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO6653200.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29442..29765)
                     /gene="iscA"
                     /locus_tag="ACHKUB_000116"
     CDS             complement(29442..29765)
                     /gene="iscA"
                     /locus_tag="ACHKUB_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO6653201.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29782..30168)
                     /gene="iscU"
                     /locus_tag="ACHKUB_000117"
     CDS             complement(29782..30168)
                     /gene="iscU"
                     /locus_tag="ACHKUB_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO6653202.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30196..31410)
                     /gene="iscS"
                     /locus_tag="ACHKUB_000118"
     CDS             complement(30196..31410)
                     /gene="iscS"
                     /locus_tag="ACHKUB_000118"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO6653203.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31522..32010)
                     /gene="iscR"
                     /locus_tag="ACHKUB_000119"
     CDS             complement(31522..32010)
                     /gene="iscR"
                     /locus_tag="ACHKUB_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO6653204.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32311..33048)
                     /gene="trmJ"
                     /locus_tag="ACHKUB_000120"
     CDS             complement(32311..33048)
                     /gene="trmJ"
                     /locus_tag="ACHKUB_000120"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO6653205.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33167..33970
                     /gene="suhB"
                     /locus_tag="ACHKUB_000121"
     CDS             33167..33970
                     /gene="suhB"
                     /locus_tag="ACHKUB_000121"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO6653206.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34115..34969
                     /locus_tag="ACHKUB_000122"
     CDS             34115..34969
                     /locus_tag="ACHKUB_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6653207.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35160..36440
                     /gene="csiE"
                     /locus_tag="ACHKUB_000123"
     CDS             35160..36440
                     /gene="csiE"
                     /locus_tag="ACHKUB_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO6653208.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36432..37571)
                     /locus_tag="ACHKUB_000124"
     CDS             complement(36432..37571)
                     /locus_tag="ACHKUB_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO6653209.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37731..38621)
                     /gene="hcaR"
                     /locus_tag="ACHKUB_000125"
     CDS             complement(37731..38621)
                     /gene="hcaR"
                     /locus_tag="ACHKUB_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO6653210.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38757..40118
                     /gene="hcaE"
                     /locus_tag="ACHKUB_000126"
     CDS             38757..40118
                     /gene="hcaE"
                     /locus_tag="ACHKUB_000126"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO6653211.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40115..40633
                     /gene="hcaF"
                     /locus_tag="ACHKUB_000127"
     CDS             40115..40633
                     /gene="hcaF"
                     /locus_tag="ACHKUB_000127"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO6653212.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40633..40953
                     /locus_tag="ACHKUB_000128"
     CDS             40633..40953
                     /locus_tag="ACHKUB_000128"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO6653213.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40950..41762
                     /gene="hcaB"
                     /locus_tag="ACHKUB_000129"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40950..41762
                     /gene="hcaB"
                     /locus_tag="ACHKUB_000129"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO6653214.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41772..42974
                     /gene="hcaD"
                     /locus_tag="ACHKUB_000130"
     CDS             41772..42974
                     /gene="hcaD"
                     /locus_tag="ACHKUB_000130"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO6653215.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43071..43493
                     /locus_tag="ACHKUB_000131"
     CDS             43071..43493
                     /locus_tag="ACHKUB_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6653216.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43541..44413)
                     /locus_tag="ACHKUB_000132"
     CDS             complement(43541..44413)
                     /locus_tag="ACHKUB_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO6653217.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44425..45519)
                     /locus_tag="ACHKUB_000133"
     CDS             complement(44425..45519)
                     /locus_tag="ACHKUB_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6653218.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDKVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45552..46550)
                     /locus_tag="ACHKUB_000134"
     CDS             complement(45552..46550)
                     /locus_tag="ACHKUB_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6653219.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46575..48086)
                     /locus_tag="ACHKUB_000135"
     CDS             complement(46575..48086)
                     /locus_tag="ACHKUB_000135"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6653220.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48109..49092)
                     /locus_tag="ACHKUB_000136"
     CDS             complement(48109..49092)
                     /locus_tag="ACHKUB_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6653221.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     STVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49189..52470)
                     /locus_tag="ACHKUB_000137"
     CDS             complement(49189..52470)
                     /locus_tag="ACHKUB_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO6653222.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     ATEMLNALDCQRTLSLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQSCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52588..53781
                     /locus_tag="ACHKUB_000138"
     CDS             52588..53781
                     /locus_tag="ACHKUB_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO6653223.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53845..55098)
                     /gene="glyA"
                     /locus_tag="ACHKUB_000139"
     CDS             complement(53845..55098)
                     /gene="glyA"
                     /locus_tag="ACHKUB_000139"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO6653224.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55426..56616
                     /gene="hmpA"
                     /locus_tag="ACHKUB_000140"
     CDS             55426..56616
                     /gene="hmpA"
                     /locus_tag="ACHKUB_000140"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO6653225.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56661..56999)
                     /gene="glnB"
                     /locus_tag="ACHKUB_000141"
     CDS             complement(56661..56999)
                     /gene="glnB"
                     /locus_tag="ACHKUB_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO6653226.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57060..58394)
                     /gene="glrR"
                     /locus_tag="ACHKUB_000142"
     CDS             complement(57060..58394)
                     /gene="glrR"
                     /locus_tag="ACHKUB_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO6653227.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58384..59097)
                     /gene="qseG"
                     /locus_tag="ACHKUB_000143"
     CDS             complement(58384..59097)
                     /gene="qseG"
                     /locus_tag="ACHKUB_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO6653228.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59262..60689)
                     /gene="qseE"
                     /locus_tag="ACHKUB_000144"
     CDS             complement(59262..60689)
                     /gene="qseE"
                     /locus_tag="ACHKUB_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO6653229.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61265..65152)
                     /gene="purL"
                     /locus_tag="ACHKUB_000145"
                     /gene_synonym="purI"
     CDS             complement(61265..65152)
                     /gene="purL"
                     /locus_tag="ACHKUB_000145"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO6653230.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILNDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRNVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGNRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65410..66966
                     /gene="mltF"
                     /locus_tag="ACHKUB_000146"
     CDS             65410..66966
                     /gene="mltF"
                     /locus_tag="ACHKUB_000146"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO6653231.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66963..67466)
                     /gene="tadA"
                     /locus_tag="ACHKUB_000147"
     CDS             complement(66963..67466)
                     /gene="tadA"
                     /locus_tag="ACHKUB_000147"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO6653232.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGAHDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67524..68159)
                     /gene="yfhb"
                     /locus_tag="ACHKUB_000148"
     CDS             complement(67524..68159)
                     /gene="yfhb"
                     /locus_tag="ACHKUB_000148"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO6653233.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68368..69216
                     /locus_tag="ACHKUB_000149"
     CDS             68368..69216
                     /locus_tag="ACHKUB_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6653234.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(69921..70592)
                     /locus_tag="ACHKUB_000150"
     CDS             complement(69921..70592)
                     /locus_tag="ACHKUB_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO6653235.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGIGRVASVLWPVNQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            complement(70634..72361)
                     /locus_tag="ACHKUB_000151"
     CDS             complement(70634..72361)
                     /locus_tag="ACHKUB_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6653236.1"
                     /translation="MTVRISGVLKDGTGKPVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERG
                     PKGERGERGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAAT
                     PKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFVA
                     WLKEKGAFDQPYWMMKASLHAGFNKVITDVGPGKLNLGGCVIEVMGKYEAAIVRVTIG
                     EYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDT
                     GSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGETGA
                     TGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGNKQTYAPQT
                     DSTTWAVDLDIGAMITGIGGYNDGNKTLIDRVSYRPLQVTFDGSQWRTVSVGEYVSSG
                     ASGFEYFPL"
     gene            complement(72504..72644)
                     /locus_tag="ACHKUB_000152"
     CDS             complement(72504..72644)
                     /locus_tag="ACHKUB_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6653237.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQC
                     KQ"
BASE COUNT        17075 a        20293 c        18221 g        17227 t
ORIGIN      
        1 tgaaatttaa aaaatgtctt ctgcctgtgg caatgttagc gtcattcact ctggcaggat
       61 gccagtcaaa tgctgacgat catgctgccg atgtttatca aaccgatcaa ctgaatacca
      121 aacaagaaac taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca
      181 actcccaaaa taaacggaac gcacaagcct tcggcgcgct tattggcgca gtcgctggcg
      241 gtgttatcgg ccacaacgtc gggtctggca gcaattccgg aacgacggca ggtgcagttg
      301 gcggcggagc tgtaggcgcg gcagcgggtt ctatggtgaa tgataaaacc ttagtggaag
      361 gtgtttcttt aacctataag gaaggcacca aagtgtatac ctctacccag gtgggtaaag
      421 agtgccagtt tacgacaggt ttagccgttg ttattaccac gacgtataac gaaacgcgta
      481 ttcagccaaa taccaaatgt cctgaaaaga gctaataatc aggaggagtc atgaagaaag
      541 tttttctttg cgccatctta gcctccttaa gctatccggc tatcgcctca tcattgcagg
      601 atcaactctc tgctgtcgca gaagcggaac agcaaggtaa aaatgaagag caaaggcagc
      661 atgacgaatg ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa
      721 atgcccaggc cgccgctaac aaaagagcgg caacggcagc ggcaaataag aaagctcgtc
      781 aggataaact ggacgccgaa gccactgcgg acaaaaaacg cgatcaaagt tatgaagatg
      841 agctacgcag cttagagatt cagaaacaaa aactggcgct ggcgaaagaa gaagcccgcg
      901 ttaagcgcga aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg
      961 tggtgcaatc tgaagctgac gccaacagaa atatgactga aggcggtcgc gatctgatga
     1021 aaagcgtggg caaagcagaa gagaacaaat cggaaagctg gtttaattaa tcgatgttag
     1081 taacttcaag cctatgattc ttgaagataa aaaaccctct gtagtaacag agggttttgt
     1141 tcattcatag tgcagggtca aatcattccc actcaatggt agctggcggc ttgccgctga
     1201 tgtcatacac cacgcgagaa ataccgttca cttcattgat aatgcggttg gaaacacgac
     1261 cgaggaaatc gtacggcaga tgcgcccagt gtgcggtcat aaagtcgatg gtttcgacag
     1321 cacgcagaga gacaacccag tcatacttac gaccatcgcc cattacgcca acggaacgta
     1381 ccggcaggaa cacagtgaac gcctggctga ctttgtcgta caggtccgct ttacgcagtt
     1441 cttcaatgaa gatggcgtca gcacggcgca gcaggtcaca gtactctttc ttcacttcac
     1501 ccagcacacg aacgccaagg cctggtcccg ggaacgggtg acggtacagc atgtcgtacg
     1561 gcaggcccag ctccagacca atcttacgca cttcgtcttt gaacagctct ttcagcggtt
     1621 caaccaggcc catcttcatc tctttcggca ggccgcccac gttgtggtga gatttgatga
     1681 cgtgtgcttt accggttgca gaagccgcag attcgataac gtcagggtag atggtgccct
     1741 gcgccagcca cttcacgtct tccagtttca gcgcttcttc atcgaacact tcaacgaaaa
     1801 cgcgcccgat gattttacgt tttgcttccg gatcgttttc gccagccagc gctgacagga
     1861 agcgatcttc tgccggtacg tgaacaatgt tcagaccaaa gtgatcgcca aacatatcca
     1921 gaacctgctc tgcttcgttg agacgcagca ggccgttgtc gacgaatacg caagtcaggt
     1981 ttttaccgat agcgcggtgc agcagcattg cggttacgga ggaatccaca ccaccagaga
     2041 ggccgaggat gactttatcg tcgcctacct gctcgcggat gcgagctaca gcatcgtcga
     2101 taattttcgc tggcgtccac agggcttcac actggcagat atcacgcaca aaacgctcca
     2161 gcatgcgcat accctggcgg gtgtgagtca cttccgggtg gaactgtacg ccatagaagc
     2221 gtttttcttc gttagccata atggcaaacg ggcagctttc ggtgctggct acggtgacga
     2281 agtcggacgg gatagcggta actttatcgc cgtggctcat ccagacatcg agcagcggtt
     2341 taccgtctgc ggtcagcgca tcttcgatac cgcgaaccag tgcgctgtcg tttacgactt
     2401 caacctgcgc gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg cccaactgca
     2461 ttgccatggt ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct tcaaagacat
     2521 actgcggcgc acgcgggctg ttttcttcgg tggtgctttc cgggccgccg gaaagaataa
     2581 tgccgcttgg attgaagtca cgaatttgtg cttctgtcac atcccacgcc cacagttcgc
     2641 agtaaacacc cagctcacgc acgcggcgcg caaccagttg agtgtactga gaaccgaagt
     2701 ccagaatgag gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa
     2761 gtgaaacaga taatataaat cgcccgacat gaagtcgggc gaagagaatc aggagcccag
     2821 acggtagttc ggggactctt tagtaatggt cacgtcgtga acgtggcttt cctgaatgcc
     2881 cgcaccgctg atacgtacaa actccgcttt agtacgcagt tcgtcgatag taccacagcc
     2941 ggtcagaccc atacaggagc gcaggccgcc catctgctgg tgaatgatct ctttcaggcg
     3001 acctttataa gctacgcgac cttcgatacc ttccggcacc agtttgtcgg cagcgttatc
     3061 gctctggaaa taacggtcag aggaaccttt ggacatcgcg cccagggaac ccataccacg
     3121 gtaagatttg taagaacggc cctggtagag ttcgatttca cccggagatt cttcagtacc
     3181 cgccagcatg gaacctacca tcaccgcgct tgcgccagcg gcgatagctt tggcgatgtc
     3241 gccggagaag cgaataccac catctgcgat aaccggaata ccggtgcctt ccagggcttc
     3301 aactgcgtca gcaacggcag tgatctgcgg aacacctacg ccagtaacga tacgagtcgt
     3361 acagatagaa ccagggccga taccgacttt aaccgcgctg caaccagctt ctgccaaagc
     3421 gcgtgcacct gcagctgttg ccacgttgcc gccgatgatt tgcagatccg gatatttagc
     3481 acgggtttca cggatacgct gcaaaacgcc ttctgagtga ccgtgggagg agtcaatcag
     3541 cagaacgtca acgccagcgg caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc
     3601 gccaactgca gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg
     3661 ttccgctttc tggaagtctt tcacggtgat catgccgatc aggtggaatt cgtcatcaac
     3721 caccagcgct ttttcaacgc gtttttcgtg catttttgcc agcaccactt cacgggcttc
     3781 accttcacgc acggtgacca gacgctcttt cggcgtcatg taaacgctaa ccggctggtt
     3841 caggtcggta acaaaacgca cgtcacgacc ggtgataata cccaccagtt cgttttcttc
     3901 ggtaacgacc ggatagcccg caaaaccgtt acgctcagtc agttctttca cttcgcgcag
     3961 cgtcgtggtt ggcagaacag tctgcggatc agtcaccaca ccagattcgt gttttttcac
     4021 acggcgaact tcttctgcct ggcgttcaat ggacatgttt ttgtggataa agccgatacc
     4081 gccttcctga gccagagcaa tagccaggcg cgcttccgtt acggtatcca ttgctgcgga
     4141 aagcataggg atattcagac gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt
     4201 cggcagaacg gtagaatgag caggaacgag gagaacgtcg tcaaacgtca gagcttcttt
     4261 agcgatacgt agcatgggca atatctcgac cagagtggtt aataaatatt gccgcggcat
     4321 tatacagagc gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg ctatccccga
     4381 agggcgggtt actatcgact gaataacctg ctgatttaga atttgatctc gctcacatgt
     4441 taccttctca atcccctgca atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc
     4501 tgcttgagca tgagatggga caggtttgga tcagcggcga aatctctaat ttcacacaac
     4561 cggcttccgg tcactggtac tttacactca aagacgacac cgcccaggta cgctgcgcga
     4621 tgttccgcaa cagcaaccgc cgggtgacct tccgtccaca gcatggacaa caagttttag
     4681 ttcgcgccaa tattacgctc tacgagccgc gcggtgacta ccagataatc gttgagagta
     4741 tgcagccggc cggtgaaggg ctgctgcaac agaagtacga acagctcaaa gcgaagttgc
     4801 aggctgaagg tttgttcgat cagcaataca aaaaaccact tccctcccct gcgcattgcg
     4861 ttggtgtgat cacctcaaaa accggtgctg cgctacatga tattttgcat gtgttaaaac
     4921 gtcgcgatcc ttctctaccg gtgatcatct accccaccgc cgttcagggc gatgacgcac
     4981 cggggcaaat tgttcgcgcc attgaactgg cgaatcagcg caatgagtgc gacgtgttga
     5041 tcgttgggcg cggcggcggt tcgctggaag atttatggag ttttaacgac gaacgcgtag
     5101 cgcgggcgat ttttgccagc cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg
     5161 tgaccattgc cgattttgtt gccgatctgc gtgcgccaac gccgtctgcc gccgctgaag
     5221 tagtgagccg taatcagcaa gagttactgc gccaggtgca atcgacccgt caacggctgg
     5281 agatggcgat ggattattat ctcgccaacc gcacgcgtcg ctttacgcag atccatcacc
     5341 gattacagca acagcatccg cagctccggc tggcacgcca gcaaaccatg cttgagcgcc
     5401 tgcaaaagcg gatgagcttt gcgctggaaa atcaacttaa gcgtaccggg caacagcagc
     5461 agcggttaac acagcggctg aatcagcaaa atccacagcc gaagattcat cgcgcgcaaa
     5521 cgcgcattca gcaactggaa tatcgtttag cagaaaccct gcgcgtacag cttagcgcca
     5581 cgcgtgaacg tttcggtaat gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc
     5641 tggcgcgtgg atacagcgtt actactgcta ctgacggcaa tgtactgaaa aaagtgaagc
     5701 aagttaaagc gggtgaaatg ctaaccacac gtctggaaga cggctggata gaaagtgaag
     5761 ttaaaaacat ccagccagta aaaaaatcgc gtaaaaaggt gcattaagcc agcacgaatt
     5821 caacgcgttt tttcgatatc aagccatgcc cgtgctgaca gaagtaatct actgcaccgc
     5881 aggcttttaa cacctgcaac ggttgatggc agtctggaca aagagctttc atttcaataa
     5941 tttccccgca ggatgggcaa cgcgcatggt cgttatcctg atcaagcaca tgctgacatt
     6001 gtggacaatg cagttccata tgaattcctc cggtagccat ccctctacat tcatagaggg
     6061 aatggcagat aaaatactta cggataatta tttatttttc ttgatgtgct tcatcagacg
     6121 cttacgttta cgcatctggg ttggcgtcag ggtgttacgc ttgttcgcat acgggttttc
     6181 cccttctttg aactgaatac gaatcggcga tcccattacg tccagcgatt tgcggaagta
     6241 gttcatcaag tagcgcttgt aggaatcagg caggtctttc acctgattac cgtgaatcac
     6301 cacaatcggc gggttataac caccagcgtg ggcatatttc agcttcacac gacgaccgcg
     6361 taccagcggc ggttggtgat cttcaacagc catcgtcatg atgcgcgtca gcatagaggt
     6421 ccccacacga cgggtggagc tgtcatacgc ttcacgtact gattcaaaca agttaccaac
     6481 accactgccg tgcaaggcag agataaagtg cacacgagca aaatcgataa agcccagacg
     6541 gaagtccagc gtttctttca cctgctcttt cacttcctgg ctcaggccat cccacttatt
     6601 caccacaatg acaagtgagc gcccactatt gagaataaag cccagcagcg agagatcctg
     6661 atcggaaata ccttcgcgcg catcaatcac taacatcacc acgttggcat cttcaatggc
     6721 ctgcaacgtt ttgattacgg agaatttctc tacagcatcg gtgattttgc cgcgtttacg
     6781 tacgccagcg gtgtcaatga gcacatactc acgtccatcg cgttccattg gaatgtagat
     6841 actgtcacgc gtcgtgccag gcatgtcata aacaacaacg cgctcttcac caagaatacg
     6901 gttagtgagt gtagacttac ctacgttcgg acgacccaca atcgccagtt tgatcggcag
     6961 actttgcggg tcgaagtcgt cttcctcttc ttcttcgccg ttctcttccg cttcaaattg
     7021 cgcccagtat tcagcgtctt cgtcgacttc ctcttgcggt gcgagatctt ccatccacgg
     7081 cagcagcaca tgctccagca gacttaatac gccacgaccg tgagacgcgg cgatcgggta
     7141 gatttcacct aagccaagcg agtagaaatc aaccactgcc tgatcgggat ccagaccgtc
     7201 agttttgttt gccaccagga aggtcggttt ttcacgggag cgcagatgtt tggcaatcgc
     7261 ttcatctgcc ggcatcaggc ccgcgcgcgc atccaccata aacagtacga cgtccgcttc
     7321 ttcaatcgcc agcagcgact gttccgccat gcgggtttct acaccgtctt ctgtgccatc
     7381 aatcccgccg gtatcaatac agataaactc acggccttca atttccgcac gaccgtactt
     7441 acggtcacga gtcagacccg ggaaatccgc aaccagcgca tctcgggtgc gagttagacg
     7501 gttaaataac gtggattttc ctacgttagg gcgcccgaca agcgcgacca caggtaccat
     7561 gtttaaagcc tcatttttat aaatcatcag acaacgcacg ctatattcgc gtcgttgtta
     7621 aaaacaggaa aacggcccct gtccaggagc cgttttcaaa gtgaacgaca gagacgatta
     7681 acgtgtaata gagtacacgg ttccgtcttt tgcctggatc agcagtttgc cgtcagcggc
     7741 aaccggttca gtctggaaac cggaactatc aactttttgc tgggcaacga aacgaccatc
     7801 ttcgacgtta atccagtgca gataaccttc actgtcacct accaccaggt tgccattata
     7861 cagcaccgga gaagtcagca ggcgatgcaa cagatcgctt tgtgtccaca gcgtaacgcc
     7921 gccatcaatg gtcaacgcca tcacccggtc attttgatcg accagataga tgcgattgcc
     7981 gtcgacgatg aaatcattca ccgaacccag ttcgcgtttc cacataatct gaccactgcg
     8041 cagatcaagc gccgtcaggt taccattata ggccagcgcg aaaacaacgc cgttaacaac
     8101 gacgggagtc gtgtcaacat cgctcagacg gtcaatttcg gtagaaccgg tcgcctggga
     8161 aatacgctgc tgccaaatca tctggccctg ttccatcagc actgcgctga cgcgaccatt
     8221 atcgccaccc acgacggccg caccaaaagc cgttgccggc gcagactcgc cacgcaaaga
     8281 gagcgaaggc atatcgaggt taactgtcca tttgacagcg ccgtcagcct cgttcagcgc
     8341 ttgtaactga ccgttactgg tgtggattaa caccagaccg tcgctgacca ccgggcgcga
     8401 aagtgcttca cccgcgactt tagtttgcca tgccacagta ccatcgctgg tattcagcgc
     8461 gtaaacctgc gccttttcgc tgccaatgta gacatgccca ccagacacgg tcagaccgcc
     8521 agaaagtaat gcaggctctt tagagaacca gccatctttc tcggccaggc tgacagacca
     8581 gatttctttg ccatcatccg cattcagcgc ttttaccaaa ccagcgcggt ccgctgcata
     8641 gacaacgttg tccgccagtg ccggatgaag attggaatag aagttgccaa tgccgctacc
     8701 aacggaagtg ctccacgccg tggtcggcgt aaactggttt tcaacggttg gcaatgggga
     8761 catctttacc acatcttctt cgctgttaaa cagcgaacag ccgcttaaaa gggtaacgga
     8821 aagcagtcct ggcagcagta atttacgcaa ttgcatcggg tccctctcag atggacaaat
     8881 tattaatttt catctgcatc atttcgctca gtgccggagt aacatcgctt ttcacgcctg
     8941 cttcccatgc actacgcgca ccttgcttat cacctttgct cagcaatgct tcaccacgca
     9001 ggtcggcaac aatggcagcc cacccttcac ctttgatggt atcaagggtt ttcagcgcgg
     9061 catcagcctg cttgagctgt acctgaacgc gagcaagacg cagatttatc acggctttga
     9121 gattttcatc gctcgtgtct gccagcccct gttgtaactg ggcggcagct ttctctagtt
     9181 catttttgtc aacaaattgc tgcgcaagtt ccaaagaagc cagcgcacca taagtatttt
     9241 tattttcagc agcaaatttt tccgccgccg ggatgctatc cggtttgcct tcgctcactg
     9301 cggtaaccgc attttgatag gcaagagaag cggagcgtgc agaatcaacc tgatggctgt
     9361 tccagtagcg ccagccaatc agtgcgccaa cgcccaaaat caccccaaca gccagtgctt
     9421 tgccattttc agcaaaaaag cgtttaaccg cttctacctg gtcgttttcg ttctcgtaaa
     9481 tttccacgct gtccttctcc ttccttaacc cagtaacgtg cgcaaatgcg cggctacgct
     9541 atcctgcgca actgccgttt gctcaccaga gcgcaaatcc ttcactactg ctgtgccgtt
     9601 agccacttca gactcaccca gcaccacagc aacgcgggca ccccatttat cagcacgggc
     9661 aaactgtttc ttaaagttgc cgccgccgtg gttggtcatc aatttcacgc ccggtaattc
     9721 atcacgcaga cgctcagcta atgccatagc cgcagattgt gtatcagcac ctgaagccac
     9781 caggtatata tcgacaacag gatcggcttt aaattccgga ttaacggcct gtactaacaa
     9841 tacaagacgt tcgaggccca tcgcaaaacc gacagccggt gttgcacgac cgcccagttg
     9901 ttccacaaga ccgtcataac gaccgcctgc acacacggtg ccctgggagc cgagactgtt
     9961 agtcacccac tcgaaaacgg tacggttata gtaatccaga ccacgcacca gacgctggtt
    10021 tacggtgtaa gcgatccccg cgctctcaag cagtttgcac agaccggcaa aatgctcacg
    10081 agattcctcg tccagataat cacctaatgc cggagcgtcg ttgagaagcg cctgcacttc
    10141 cggatttttt gaatccagca cgcgcagcgg gttagtgtac atgcggcgtt tgcagtcttc
    10201 gtccagcttt tctttatgct gctcaaggaa tgccaccagc gcatcgcggt aattggcgcg
    10261 tgcttccagc gaaccgatag aattcagctc aagagttacg tgttcggaaa tacccagcgc
    10321 gcgccaccag cgggcggtga gcataatcag ttcagcgtcg atatccggac cttgcagacc
    10381 gaaaacttcg cagcccaact gatggaactg acgataacgc cctttctgcg gacgctcgtg
    10441 acggaacatc ggcccgatat accacagacg ctgttcctga ttgtacagaa gaccatgctc
    10501 aatgccggcg cgtacacagc ccgccgtccc ttcagggcgc agagtcaggc tgtcgccatt
    10561 gcgatcctca aaggtgtaca tctctttttc aaccacgtcg gtgacttcac caatcgcacg
    10621 tttgaatagc ggggtctgct ctacaatcgg caagcggatt tcactgtaac cgtagctgcc
    10681 gagcacgttt ttcagtgtgc cttcaatgcg ctgccagatg gccgtttcgc caggcaggta
    10741 atcgttcatg ccgcgaatgg cttgaatgtt ttttgccacg tttattctct ttctgaatat
    10801 aaaaatgaac cctcaacgct tccctcaatg tttcgggagc tatgcgggtt caatcataca
    10861 cgggaagcga ggcgcttccc atcacgttat tatttttcaa cctgctgaac gtcaattcga
    10921 cgcgcttcgt ccagctgact ggctttcgca cgaatgcgtg cttccagctg gtcgatcata
    10981 tcgttgttgt ccagacggtc tttgcgcacg ccatcttcat agaggccgct tttcttgttg
    11041 ccgccggtga cgccgagtgt agaaaccagc gcctcacctg ggccattcac cacgcagccg
    11101 ataatcgaaa cgtccatcgg agtgatgata tcttccaggc gttgctccag cgcgttaacc
    11161 gtaccgataa catcaaattc ctgacgcgaa caggtcgggc aggcgatgaa gttgatcccg
    11221 cgcgaacgga tacgcagcga tttcaaaata tcgaaaccga ctttgatctc ttcgaccgga
    11281 tcggccgcca gtgatacgcg cagcgtgtcg ccgatgcctt cagacagcag cagacctaaa
    11341 ccaatggcgg attttactgc cccgctgcgt gcaccaccgg cttcggtgat ccccagatgc
    11401 agcggctgat cgatctgttt tgccagcaaa cgataagact caacagcgag gaagacgtca
    11461 gacgctttca cgctgacttt gaactgatcg aagttcaggc gatcgagatg atcaacatgg
    11521 cgcatggcgg attccagcaa cgcctgcggc gtcggttcgc catacttttc ttgcagatct
    11581 ttttccagcg atccggcgtt aacgccaata cggatcggaa tgtttttatc gcgcgcacag
    11641 tcaaccacca tgcgaatacg ctcttcatta ccgatattgc cagggttaat acgcagacaa
    11701 tcgacgccgt attccgctac tttcagtgca atgcgatagt cgaagtggat gtcagccacc
    11761 agcggcacgt taacctgctg tttgatgagt ttgaacgctt ctgccgcgtc catcgtcggt
    11821 acggagacac ggacgatatc agcgccaacg cgttccagcg ccttgatttg attgaccgtt
    11881 gcttcgacgt ctgtcgtacg cgtattggtc atggactgta cggcgatggg agcaccatcg
    11941 ccaatcggca cattcccaac gtaaatacgt gttgattttc tacgttgaat tggagcctgg
    12001 ttatgcatga aaaatctccc gcgttacccg tctgttactg cgccggtgat tgttcggcat
    12061 tgagggtcag acgcgcaacc tggttagttc tgataaaacg actcagatcg acaggtttcc
    12121 cttgatactg gatctgtact gcggctggcg caccaatttt cagtttgtat ggtgcctggc
    12181 cggttaagtt caaattaccg tctttacgct gcataccgct aaacaatttt ttaccggtag
    12241 catcagtaac ctccagccag caatcggcag taaagttcat caccagcgca ttcggatcag
    12301 ccgccggcgt agtcacgcct gcctgatcgg ttggcaacgg cgcagcgcca tctggcgttg
    12361 ttgttgccgt tggtgccggg gtcgcggcgg tatcaacatt tgcctgcgaa ggcgaaacaa
    12421 ccgcattctg ttgcggatca acagctggtg ctggcgcagt tacggcaggt gtttgcgtgt
    12481 tggttgcggt agtatccaca gacgctggcg gcgtggacgt tgtagccggg tctgtagttg
    12541 tcgacgtatt taacggaaca ctctgcccct gctcgctatt actgctcagt tccgccgaag
    12601 attgatcggc catagtggtg atctcttcct gctgagcttt gtggtcttgc caccaccagg
    12661 caccgctcag gccgataacc acaaacaaca ccagccaggt gaaggtcatc agccagccgt
    12721 cacgtttttt gcggcgttta ccgagggaaa aactctgcat cggcgcaact ttcgcagccc
    12781 gtagtggagc ctgcttttcc agccctggca gcagttcttc ttctggaata tgtaccagac
    12841 gcgcataaga gcggatatat ccgcgcagga atgttgaagc aagatcggcg ggtgccttat
    12901 cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca acggcctgct
    12961 gactaagtcc tagttgttcg cgagcattac gcaggcgagc gccggtagta agtgcttcat
    13021 tttggtcgtg cgtggcttca gtattcattc gctgcaggta cgtttaaatg agaattgaga
    13081 tgccggtgaa ccatcatgct cacccacacc gcgaaacatc cgttaagtta accgttatca
    13141 tacagtataa gaccgtctgt gtgctcgtga caaagcccac attcactaag gctaacttac
    13201 tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg caccgttaat attgaccaga
    13261 caattgcaac ttaatacttc attcataaaa gattacgcca cggtacataa agtaaccgtg
    13321 gcgtaatggc tatcagaccg ctttaatgtc gatggcttca ccctgcatcc gtttacgcag
    13381 ggtacgtttc gtacggtcga taacatcgcc cgccaactga ccacaggcag catcgatatc
    13441 atcaccacgg gttttacgca caatagtggt gaaaccgtag ctcatcagca cttttgagaa
    13501 acggtcgata cggctgttcg agctgcgtcc atacggcgcg cccgggaacg ggttccacgg
    13561 gatcaggttg atcttacacg gcgtatcttt cagcagttcc gccagttggt gcgcgtgttc
    13621 agtgccgtcg ttaacgtggt caagcatcac atactcaata gtgacacggc cctgattggc
    13681 gttggatttc tccagataac ggcgcaccgc agcaaggaac gtttcgatat tgtacttttt
    13741 gttgatcggc acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg aaattgccag
    13801 tgcaacgtcg atcatatcgc ccagtttatc cagcgccgga actacaccgg aagtagaaag
    13861 cgtgacgcga cgtttagaca ggccaaaacc gaaatcatca agcatgattt ccatcgccgg
    13921 aacgacgttg ttcaggttga gcagcggctc gcccatgccc atcatcacta cgttagtgat
    13981 tgggcgctga ccggtgactt ttgctgcgcc gacgattttc gccgcacgcc acacctggcc
    14041 aataatttcc gatacccgca ggttgcggtt aaagccctgc tgggcggtgg aacagaattt
    14101 acactccagc gcacacccca cctgcgaaga gacgcagagc gtggcacggt cgtcttccgg
    14161 gatatacacc gtttcgacgc gctgatcgcc tacggcgatc gcccatttaa tggtgccgtc
    14221 ggatgaacgc tgttcttcaa ccacttccgg tgcgcggatt tccgccacct ctttcagttt
    14281 gccgcgcaac actttgttga tgtcggtcat ctcatcaaag ttgtcgcagc aatagtgata
    14341 catccacttc atcacctgat cggcgcggaa gggtttttca cctaaatctt taaaaaactc
    14401 ccgcatctgc tgacggttga gatccagcag gttgattttt ccatctttcg tggtgacgtt
    14461 ttcaggtgtg actaattgtt cagacatatg ctattccggc ctcgttatta cacgttatgg
    14521 cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg ggggcgctgc
    14581 attgtacaaa ttctggcgca cggatgccac gtttgcacgc ggcatttacg aaattattaa
    14641 cgggtgcgcg ggcacacttc gccttcgcca aagaaataag cgatttcgcg agcggcagat
    14701 tcgacggaat cagaaccgtg ggtaccgttt tcggtcaggc tgtcagcgta atcagcgcgc
    14761 agagtaccag ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg gtgacgctga
    14821 acggcgtttt caccttccag cacggaaacc acgatcgggc cagaggtcat gaattcaacc
    14881 agaccatcaa agaacggttt tccatcgtgt tcagcataaa agccacgtgc ctgttcaacg
    14941 gtcaggtgca gcattttggt gccgacaatt ttgaaccctg cagcttcaaa gcgcgcaaag
    15001 atattaccaa tgacgttttt tgctaccgcg ttcggtttga tgatggaaaa agtacgttca
    15061 atagccattt ttacctctgt aaattgttct gttgttgtct gtaccagcgt acgagatggc
    15121 gcggattata atgagcaaca gggccgttga ctattgatga aggtaacatt tttttaaaat
    15181 aaaatgagtt ttagcaacaa tcaggagcaa aacagactat tgcatgacaa atttcactgt
    15241 cgcgatttgc cccacatcat ccatcaccag caattgataa tcacctttat ccgtcaaatg
    15301 cagggtcacg ttgcgcccgc gttcagttaa cggttcgcca ttcagaaacc accagcgttc
    15361 acctgcccct ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg gtaaacgttt
    15421 aataatcgcg ccatcgcgaa cgcctgtcag ctgcagaggg agcttggcat cgtgaccgta
    15481 tggcgggcaa attgtcgagg ccggtggtaa gcgcacagcg cgacgttctg atgcgggcag
    15541 ccagggttcc agcggtagcg gccagacatt tatcatttct tgtcgcgctt gcgggcaatc
    15601 agcggcaaca cgtttgccat tttcatccag ccagatgggg aaacgaatgc cattaatgcc
    15661 ttcctgctcc ggcagtaata gagttggcgg ctgactcccg tccagcagcc aggttgccag
    15721 gcggcggcga cagttaccgt caccttccgg caaagactgt ccgcccggcc agcagataac
    15781 gccacgagtg actgagttcg ggcgcgggtc ttccggcaga ttcgcactgc gcgacagtaa
    15841 gatattattg acctgattca acaatggtac ggcactggca aagccaaact gaccaacaac
    15901 gggcgtgccg tccggtctgc cagtccagat cccaatgaca tagcgagcgt taaccccaat
    15961 cgcccaggcg tcacgatagc catagctggt gcccgttttc catgccagtg gggcgacgcg
    16021 cggcaaggca ctatccggca agggtttcgc ttcatcagcc ataatccggc gaatgatcca
    16081 cgccgccccc gacgacatta aaggccgttc aagcagcgga tcgtcaggct gtaagcgcaa
    16141 tttgcctgcc ttgccgtggc gagcaaacgc ggtatacgct gccgccatat cttccagttt
    16201 tgcaccagcg ccgccgagaa tgagtgaaag attcggcgca gcaccgttgg gcaaatataa
    16261 cggcaatcca acattgcgta actttgccgc aaaccgtttc ggtccatagg cttccagcac
    16321 ctgcacagca ggtaagttca gcgagcgcac cagcgcctcg ctcatgctga tcgggccatg
    16381 aaaaccgcta tcaaagttac ctggtcgata atcaccggtg cgccggggga cgtcttgcag
    16441 cagtgatgcc gggtggatca agccttcatc cagcgccaga ccataaacaa acggtttgag
    16501 caccgatccc ggcgatcgga tcgcattgac catatcaaca tgaccaaagc gtgaatcatc
    16561 gttgagatca accgatccca cccagccgcg aacacgcata tcggtatgat caaccacgat
    16621 catcgccagt gagctgcgcg gtggcaaccg ccctttccag ttttgcgcca gttcttccag
    16681 acgtcgttga agaccggcat ccagcgtagt aacgattttg tcgcttttgc ttttaccgag
    16741 catcatgcgc gaaaacagcg gtgccagttg cggcatttgt cggggtgcca gccagatggg
    16801 ttcttccctt gactctttta cctgctcacg ggaccacaca ccttgcacgg ccatccgttc
    16861 gagcacttta ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc gaagacggct
    16921 gggcgcttgc ggcaaaaccg ccagcattgc cgcctcggaa tagcttaaat tcgcaggcga
    16981 ctttccgaga taagcccaac ttgccgcacc gatcccctgc aacgtaccgc caaacggagc
    17041 gcggttaaga tacaaggtca gaatttcacg cttagacaga tgccattcca gttgcaacgc
    17101 gcgccagagc tggcgaattt tgccgccaaa tgttttgggg tgaggatcaa gcagacgagc
    17161 aacctgcata gtgagcgtgc tgccaccgga aataacccgt cccgaagtga gatcttgcca
    17221 tgctgcgcgc gccaccgaga atggattcac ccccggatgc ttccagaacc agcgatcttc
    17281 ataattgatc agcgcttcaa ggtaacgtgg agaaacatct tcgattgtta ccggataacg
    17341 ccagatgccg tcagcatcgg cgaagcgcca tagcggcgta ccatcctgcg ccacgaccac
    17401 tcgtgcggga ttgacttcat gcagcggtag aggccagagt ttatctgctc cccacgctgc
    17461 aagaaaaagg agaaatggcg cagctgccag cgttatccag cagccgcgtt tggttaacaa
    17521 gcgaggcatt tacggtcgga caatcagcag atcttcagcc gcgccggtcg cccgccattg
    17581 gggaacatac attgattcca ccatcggttg cggtacctga tacgttcccg gcgtcaccgc
    17641 ccgcgccaga tacaccagcg ttaccggttg gtattcatca acggcaaccg ccgccacaaa
    17701 gcgatcgtca cggaactcaa tgtgcttaat gctcgcctgc tgcatctggt tcagtaagtt
    17761 ttgcacttcg ccaccacttt gctccaggct ggcgctaccg ttcgccagat tctgattttc
    17821 cagttccaga cccgcaggca gcagatccac gactaacgca tccggcacgc tgttactggc
    17881 ttttacctgc aaccagacca gcaccagatc gccgctacgt aacgagtcca gcgatttgct
    17941 cttaccatca gtaccaagaa tatgacgctc gatttgcagc acattgttcg ccggtaaagg
    18001 tgcggattgc ggataaccgc tggcatccat acgcaaccat aacggctgat cgccactgtt
    18061 tctcacctgc aaggtggcaa gttgatcgct attcagattg ctgtttagcg ttttctcgcc
    18121 tgtcagcggc tcagctgaga aagaggtttg cgcctgccat ttacccggta aatcctgaat
    18181 cgtacgggca gccaggaaca acgcgttact ttcctgcgtc gatagccagc gttcaccaaa
    18241 cgcctgctgc gaaagtgtgt tcagcaaagt gtactgctca tcgggtagta gtttattttc
    18301 ttccagcaag gagagcatta acgcgttgtc gcgcagtgga ctaccgtaat cacccagcca
    18361 tatccgctca tcactatttc tcggcgtttt cagcgccagc acaatcgctt cttcaccacg
    18421 cgttgcatca cccatggttt tcagcgcaac gccaagttgc agcagcggta aaccagaagc
    18481 ggcatctgca cgatgctccc agatttcacg cagcgcaccc agtggtgctt tttgctgacg
    18541 ggccagcacc agcgcagcgt aagactgaac ggcgaattta ctggctttga gattatccgc
    18601 gtacgggatc gacatcatgc ccggatcttg taaatagcgc agcagacgct cattaccccg
    18661 gttaatggcg tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca ggaaatccat
    18721 cacgtaagcc gtcagccagt actcttcgtc accgttttta tcccacagcg caaagccgcc
    18781 gttatcacgt tgcatttgca gcaaacggga aatgccgata tcgaccgatg cacggcgttt
    18841 ctcatcactg tcgcctttga tgcccaacgc ctgcagttgg gctgcgttgg tataaagtga
    18901 cgggaacagg ccgctggcgg tttgctcaag acagccgtac ggatacgctt ttaactcttt
    18961 gatataacgt gcaatgttca gcggtggttt gccgctcaac aacaattgcc cttccagcgt
    19021 aacaggcgag aagttttgca atccatccgc cggaatcgcc catgtctcac caggctgtaa
    19081 cgccgtaccg taattaaccg tttgtgccgg gaacgccgga cggacgccga ttttccactg
    19141 cttatgctga tcggcaacgg tttcacccgg taacgctaac ccgctaatgg tggcctgaat
    19201 ttctccatcg ccataacccg gcaatgctcg caccgggata aacagcgtag tacgcacacc
    19261 tggcgctaat tcaacggctg cgggtgaatc gctgaccagt tcaagcaaac cactggcggt
    19321 cagggcaacg ttcagttttt gcggtttatc ggtaagatta gtgatatcca gcgtcagacg
    19381 cgaggtatcg ccactcgcca taaagcgcgg catgttcagt tcagcaatca ccggtgcggc
    19441 aactatcact ttgctttcgt tgctaccgaa atcatctgcc gtccaggctt gcgccatgac
    19501 gcgcaattca ccgttaaaat cgccaatcgg cagtgtaacc gagccttcgc cctgttcgtt
    19561 gagcgttacc ggcagcgcct gctgcgcgac aatattgacg tgattgaccg gcggtttacc
    19621 accacgtttc agctcatcac catcgccacc gaaacgcaga gctgccagac gcccctgacc
    19681 ttcaataacc tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct gaccaaagaa
    19741 cgcctgccac ggatctggcg tgacgtagtc agtaatattc agcacaccac tatcaacggc
    19801 agacaccagc acattcacct gtttaggctt ctcgccattt ttagtgctgg ctttaatttt
    19861 cacggttaat ggttgattgg gacgcatttt tgctggtgtt tccagcgcca gatcgaggcg
    19921 acggttttca tcgccaagcg gcagatgcaa cacaccaacc gcgcgttttg gcgtcgcgga
    19981 gcgagattta tcgccaggac gtaccaccag cgtacttaaa tacagatcat gacgattcca
    20041 ggttttatcg accggaatcg tcagatccag cccttgagcc cgaacatcaa tctcttgcca
    20101 ccacagcggc ccttcactgg actcgaccat cgcataacct ttacccgccg ttggcgcggc
    20161 gatatgcaac ttaatggtgt cgccagggcg ataactggct ttatccagtt tcagcgtgac
    20221 acggtcgggt cgcactgcgc cgctaccgtc gctgttgtcc tgccagctat agccagccca
    20281 gaaacgaaca ctactgaccg cttcattcgg cgctttgact tccagacgat aagcgcccca
    20341 ctctaccgga aaactgacct tgccggtttc gtccgctttc agatccagag tttgttcatt
    20401 ttcgatcaga tctttttgat caaactgtga ctgccagcct tcatcttctg accagttcca
    20461 gtagtaatcg cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg cttttttcac
    20521 gccttgcgca tcgctataaa cgatgtcaaa accggcgtta ctgccttcat caacaatggg
    20581 ctgtttcacc gtgctgtcag tacgataatc gtaaaccgat ttcgaggcga actgcggacg
    20641 gatccccggc aatgcatcgg caggccagat agcctgctca gcgcggcgcg tcaccgggcg
    20701 accgcccgat tccagcaaac taccctggaa aataacctgt aatggggaat gcgtttcctt
    20761 ccactggctt tctgtagaaa cttcgccgcg ccctttatca tccagcgtca actgaacttc
    20821 atccagcgtg cgggaaagat tttcggcagc tatatcgccg aattcaaaac caggtaaggc
    20881 tgacacagct tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat taccattagc
    20941 aggtgcacca tacaggtagt accccaccac ggagaatttc acttcatctt tcggcgttag
    21001 cggggttttc tcaccggtca gattcagcgc catgcgctct ggcataaaat cttcgacgtg
    21061 gaaatcccac atccgatact ggttatcgcc cgtgttagcg cgaatatgcc acataccggt
    21121 tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg ccattctccg gctgactaac
    21181 gacgctcctg agtacctgcc catcgggttt aatcacgtct aacttgatgg gttgattggg
    21241 caacgcttta ccgtctgcat cacgcagcaa accattgagg attaccgttt cacctgggcg
    21301 ataaagatcg cgtgggccaa acatgaaaaa ctgtttgcta tagcctggcg cgccagcaat
    21361 gttaaattct gctaagtcca gcgccggaag ttttaaatcg agtagcgttg tctgaccgtc
    21421 tttacgcgcc aacagtaatg ccgcgttttt atcattttcc agctgcacat gcccctgagc
    21481 gtcactggtt gcctgagtca gagtctgccc tttctcattt aataaagaga cttcaattcc
    21541 ttgctgggcc gcgccgtttt ccagactttg ggtaaagata tccagacgat tgtgataacg
    21601 gtgagctgaa acgccgatat cacttaacgt aaacagcgtc gcgggattac tgtaatcgta
    21661 acgtccagcc tgattcatca cagccagata cacgcccgcc tgctgaagcg gtttgatatc
    21721 gcccagcggc agcaataatt tttcacgggt gttacgcgca ggattgagat caaaccgtcc
    21781 ggtgtagacc agatccgcca tctgcagcag tttgtctgac tgccagttcg ccagcgaatt
    21841 gcggtattcc cattggctaa tgaatgctgg cagagattct ggcttaacgc ggaagaagtt
    21901 aacatcgaca ttattaacgt tgagcgccat taccggcagc ccttcaacga ctttgccagg
    21961 cagcagcgaa ccacggctgg caaaaccgac gctgggttgg atgtcgcggg tagttatagt
    22021 tttttcgtaa tctttactga aggttgcgtt gttgagtgct ttgacctcct tgccaatagt
    22081 aacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgt aactctttaa gattatctga
    22141 cagctcccag gcaccatcca ctttgccgct ttttttatcg acgacatgaa taacgcgtga
    22201 gaaatcctga tccgggtcga gagggatgga gaacgtcagc accagcgtgg ctgcaccatc
    22261 aagttggact tcagagagat ccagcaatgt cagcacctta ccggcactct gttgggccag
    22321 tttttgtctc tccggcacgg agagttttgc tgaactcgcg ttttctgaag aggccgcttt
    22381 agtaacttca gaaggcgcat cttttttcac cgctgttggc gcgttatcgt tgttgtcgca
    22441 ccctgccagc gccagcatta gcatgcaggc ggctacgcgt aactttttca tttttcatcc
    22501 ctgccacaat ggcccgttag caacgtcgaa taattattat gcgtgagaat ttacgttatg
    22561 taattcagta tcacgcccat acaatccgaa ttttagtgag aattgtggct cagacagcca
    22621 aatcagcctc tttcgcttgt cgcttttcac taaacatccg acaatttagc ctaaccccgg
    22681 caaaaatgga gatgcctatg tccacgacat ggtttgtagg agccgactgg ctcgccgaac
    22741 atattgatga cccggaaatt cagattattg atgcccgcat ggcgtcgcct ggacaggaag
    22801 atcgtaacgt tgctcaggag tatctgaatg gacatattcc cggcgcagtg ttttttgata
    22861 tcgaagcgct ttctgatcac acttccccgc ttccgcacat gctgccgcgc ccggaaacgt
    22921 tcgccgtggc gatgcgtgaa ttaggcgtta accaggataa gcacctgatt gtctatgacg
    22981 aaggtaatct tttctcagcc ccacgagcat ggtggatgct gcgcaccttt ggtgtagaga
    23041 aagtgtcgat tctggggggt ggacttgcag gctggcagcg cgatgatctg ctgttagaag
    23101 aaggtgcagt agagctgccg gaaggagagt ttaacgccgc gtttaatcct gaagccgtgg
    23161 tgaaagtaac cgatgtatta ttggcaagcc atgaaaatac ggcgcaaatt attgatgccc
    23221 gcccggctgc acgttttaac gcagaagttg atgaacctcg cccaggttta cgtcgcggac
    23281 atattcccgg agcactgaat gttccgtgga cggaactggt gcgcgaaggc gaactaaaaa
    23341 cgaccgatga actggatgcg atattttttg gtcgcggcgt cagctacgac aaaccaatta
    23401 tcgtcagctg cggctctggt gtaacggcag ccgtggtttt gttagcactc gcgacgctgg
    23461 atgtgccaaa cgtgaaactg tacgacggcg catggagtga atggggcgcg cgggcagatt
    23521 taccggttga gccagtgaaa taagctttat acaggcaata aaaaaccgcc gaatttggcg
    23581 gttttttatt gctggtctgg ttcgcggcct ttccagcagg ttgtattacc gtagtaatgc
    23641 aagcgcgtct cagcggagac aatactcgcc agtaactctc tttttgtcaa gcaaaagaga
    23701 gttattattg ttctgttagt gtattatcca ctgcggccct ttccgccgtc tcgcaagcgg
    23761 gcgctggctt aaggaaagga tgttccgcga ccgttagagc aagcgcgtct cagcgcttgc
    23821 tatcgcggca atatcgccag tggtgctgtc gtgatgcggt cttcgcatgg accgcacaat
    23881 gaagatacgg tgcttctgta tcgtacttat tgtttctggt gcgctgtttt cccaagtaaa
    23941 taataaccgg agtctctccg gcgacaattt actggtggtt aacaaccttc agagcagcaa
    24001 gtaagcccga atgccgccct ttagggcggc atattttaga ttatccggtt ttgtttaaag
    24061 tcacgcaaaa aaccacccca gcgacgttca tagaatggca caatatgttc ggtaataaag
    24121 tggctaattc ctttttcccc ttttttcacc tgacaaatat cgattggttc atcgccaggt
    24181 aatgtatcgg tcgctacact tcccgtcgcc tgaataattt cttcgatatc accatcggct
    24241 tcaatgccaa taagtaaatt aggctgtgcc tcttcgttct ctttaattga acaaataaaa
    24301 gcacgcttca ccggcttaat ggttttaaat aaggtggtga gtgaatcaat catttgtgct
    24361 ggcggctctg cgacttccga taatatcagc gattcaccgc cttccaggat ttcctggctg
    24421 ctcagcggat ttccctcttc accaatcaac aaactgattt cacgcggcat aaattcttta
    24481 ccggtcggca gtttggcatt aaggaagagc gtttcgccaa gggtcatctc aaacaatgtg
    24541 cgaacgggca ttacgacaaa tgcctgttcg tcttcaaccg cctgttgaag cgcttctaac
    24601 gaggtgaaaa aaggaatgac gctggtgccg tcttcttttt cccagtgctg taaatcaagc
    24661 gcactatctt caaccacagc ctcgccctgc gccgccgtac caggcaccca gacagtggat
    24721 tccagtagag tacggaaaaa ggccgggcgg tgcgccggtt cggttgctgc tttttccagc
    24781 aggtcttcaa gttcgttttt tgtttcggac ataagaacca caattcattc aacgttcggg
    24841 gcgcaaatgc ctgatgcgct acgcttatca ggcctacaag ggattcgcaa tttgttgaat
    24901 ttgcagaatt tgtaggccgg ataaggcgtt tacgccgcat ccggcatatt agtttacgcc
    24961 gttaacagat tagctatcgt gcgcacacca agtcccgtag cgcccgcaga ccactgttca
    25021 accggcgctt tacggtaagt cgccgagcag tcgatatgca gccagccttg ctgatagttc
    25081 tcaacaaagt gcgacaggaa gcccgccgcc gtgctcgcgc ctgccggata cgccgcgctt
    25141 ccggtattgt tcagttcggc aaagttagac ggcagctggc tgcggtggaa ttccgccagc
    25201 ggcagacgcc agaatggttc gttctcttgt gaggcactcg ccagcaaacg accggcaagc
    25261 gcatcgtcaa aactgaacag cgcgtgataa tcattaccca gcgcagtttt cgccgccccg
    25321 gtgagggtcg ccgcatcaat gatcatttcc ggtttctgcg cactggcatc aatcagacca
    25381 tcggcaagca ccagacgccc ttcggcatca gtgttcatca cttcaacttt tttaccgttg
    25441 cgataggtga tgatatcgcc cagcttgaac gcattgccgc taatcaggtt atccgcacag
    25501 cagaggaaca gcttcacgcg cttgttcagt ccgcgcgtaa tggcaaatgc cagcgccccg
    25561 gtaaccgttg ccgcgccgcc catgtccgac ttcatcgagt ccataaacgc agtctgtttg
    25621 atgctgtagc cgccggagtc aaaagtgata cctttaccta ccaggcacgc gtacaccggt
    25681 gcttctttat cgccagttgg gttgtaatcc agcgccagca ataccggaga acgttctgaa
    25741 ccgcgcccga cggtgtgcag ccccatataa ccttgctcac gcagatcttc gcctttggtg
    25801 atccgataag tcacacgacc gcccgcgacg ttgctgatca gatcaacagc acgctgtgcc
    25861 agttgcgatg gtcccaattc ttctgccggt gcattgatgg tgtcacgcac ccagtcgatg
    25921 atcatcaggc ggttatccag ttcctggcgc tgggcatcgt ccagatccgg ccactccact
    25981 ttacgcgtgc ctttcggggc tttgtaacct tgccagaatg cccagcagcg atccgcatcc
    26041 cagccttcac cgcttaactg aacatgcttg atgcccagac cgtcaatctt gcgcgccgca
    26101 cgctggatca gccccagatc gtctgccccg ttcaaatgca gggtaatgcc gtcattatta
    26161 atgctgtaag ttgctttttc tccccagcgc gcgtcggcag gttgggtaga gagggtaatc
    26221 ttcatcgctt ctgtcatttt agttatcctt ctttgtaaaa gggccgccag ttggcagccc
    26281 caggattttt ccgctaaatg attcgcgttg caggaaggcg gcaagtgagt gaagccccag
    26341 gagcatagat aactatgtga ctggggtgaa cgagcgcagc caacgcatct gcggcgtgaa
    26401 gcatgacgcg gaaattattc ggcctcgtcc agccagacta acaaaatcgc ttcgaggatt
    26461 ttctcgttgg atgcctgcgg gtcgtcgtcg aaatcttcca gatcgcaaat ccactgatgc
    26521 atatcggtga atcgaaccgt tttcggatca agatcgggat acgcatcgta cagtgcttcg
    26581 ccaatttcgc ggctatcggt ccatttaagt cccatactaa cctctgttaa tgctcacgcg
    26641 catggttgat agtgtaacgc gggatttcga ccactaaatc ttcgtcggtg acgcgcgcct
    26701 ggcagcttaa acggctttcc ggctccagtc cccaggcttt gtccagcatg tcgtcttcct
    26761 gctctgagct ttccggcagc gagtcaaaac cttcacgaac gatgcagtgg caggtggtgc
    26821 aagcacagga tttttcacag gcgtgttcaa tctcgatacc gttacgcaga gctgcgtcga
    26881 gaatggtttc accgctatta gcttccagaa cagcgccatc agggcagaga tcctgatgag
    26941 gcaaaataac aatctttggc atattaaacc tcgtccacgg aatggccttt cagcgcacga
    27001 cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg tttgtttgtc tacgttttta
    27061 atcgcttgtt cgatggcgtc aacgtcatcg ccctgcgcca cttcactcag gtgagcggca
    27121 gcatcgtcaa tgacctgacg ttctgcggcg cttaacagcg cggcatcagc agccagcgcg
    27181 ccgtgcagac tttccagcac acgcgccgct tctacttttt gttctgccag cattcgggct
    27241 tttacgtcct gctcggcata gctcattgag tctttgatca tcgaagcgat ttcgctatcg
    27301 gtcagaccgt aagacggttt gacctgaata gacgcctcaa cgccggtgga tttctccatc
    27361 gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg tcacgcgaat atgcgcacca
    27421 ccagccggta gcgccggaat accacgcagc gcaaaacgcg ccagtgagcg gcagtcctgc
    27481 accagttcgc gctcaccctg cattacatgg atagacatcg ccgtctgacc atctttaaag
    27541 gtggtgaaat cctgggcgcg ggccaccgga atcgtggtat tacgcgggat cactttctcc
    27601 accaggccgc ccatcgtttc gaggcccagc gacagcggga tcacatcaag cagcagcatt
    27661 tcgctgtccg gcttgttacc caccagaata tccgcctgaa tcgccgcgcc aatagcgacg
    27721 actttatccg ggtcgatgga agtcagcggt ggacgaccga aaaattcgcc tacccgttca
    27781 cgcaccagcg gcacacgagt agaaccgcca accatcacca cttccagcac ttcatcagct
    27841 tctacgcccg cgtctttcag cgcacgacga caagccagta aggttcgttt taccagtggc
    27901 gcgatcagtt cattgaattg ttcacggctg atttcgccct gccagcccgc aacgttaacg
    27961 gtcacggagt ccgcatcgct cagcgcgatt ttggctgcaa tggcggcatc cagcagttca
    28021 cgctgaacgc ggttatcgct acgatcagga atgtccgcct gctcgcgaat gtaatccgcc
    28081 agcagatgat cgaaatcatc gccgccgagc gcggaatcac cgccggttgc cagcacttca
    28141 aacacgccgc gacttaagcg cagaatggaa atatcaaacg tcccgccacc gaggtcataa
    28201 acggcgatca cgccttcctg accggaatcc agcccgtagg cgatagccgc agcggtcggt
    28261 tcgttaagta agcgcaggac gtgaaggccc gccagacgcg ccgcgtcttt ggtgccctga
    28321 cgctgggcat cgtcaaagta cgccggaacg gtgataacta caccatccag ctcgcctgcc
    28381 agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt ccgcagaaac gcgcaccggg
    28441 ttcagcagcc ccgccgccgt ttcaatcatc ggtaggccgt tttcgctggc ctggaattga
    28501 taaggcagat gcggatagcg ttgctggata tcagccagcg agcgccccat caggcgttta
    28561 acagaactaa tggtgttggc ggtatcgagc gctgcgttag tacgcgcgtc ataacccacc
    28621 gaatgccctt gctgttgata gtgaacaaca gatggcagca ggtgacggcc ttcatggtcg
    28681 gctaacgttt cggcctgacc gctgcgcact gtcgccacca gcgagttggt tgtgcccagg
    28741 tcaataccag ccgccagacg acgctgatgc ggcgcagcac tcaaaccagg ttcactaatt
    28801 tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg agcagttttt cttcgagttg
    28861 ttcggcactg ctccgcagtt tatcgagaaa acgcagctta cgcacggtat ccgccgccgc
    28921 gtcccacgcc tcgttgtcta actgttcaac catcaactga tggcgggtat caaacatctt
    28981 tttcacacgt ttgataaagc tttccagccg cgcttcatct ttcgcctgtt cgatctcgtc
    29041 cagctcttcg cgcagctcca actgttccat caggaacgcg gtgtcgcgca cagtatgctg
    29101 ctcgctggcg agatcaaaac cgtgtaaaga aagcaaatat tccgcgcgca ttaacggatg
    29161 acgcagcgtt tgccaggcct ggttaatggt tgcagattgc tgtacggcgg cgagttgttc
    29221 cgcctggctt ccgctggcga atttatcagg atgatactga cgttgtagat cctgaaaacg
    29281 caggctcagc gcctgggtat cgagttgata acgggcaggc aagccaaaga gggtgaagta
    29341 atccataaca ttctcagggc tgcgatccgc cgcgataacg gcgaatcagg taaaacaaac
    29401 cccacgcgca ggcgaccacg gtggggttat cggtatgcgc atcaaacgtg gaagctttcg
    29461 ccgcaaccac actcatcttt aacgttcggg ttggtgaatt taaacccttc attcaggcct
    29521 tcttttacga agtccagctg cgtaccgtcc agaaattgca ggcttttgcc atcgaccacg
    29581 actttcacgc ctttgtcttc aaacacgatg tcttccggcg tcggttcgtc aacaaattcc
    29641 agtacataag ccatacctga acatccggag gttctcacgc ccagacgcag gccaaaccct
    29701 ttgccgcggt tagccagaaa ggtatttact cgcgctgctg cactgtcgct cagtgtaatc
    29761 gacataacca aacctcaact cttattttgc ttcacgtttg cttttatagt ccgcaatggc
    29821 ggctttgatc gcgtcttctg ccagaataga acagtgaatt ttcaccggcg gcagttcaag
    29881 ttcttcagca atatcggtgt ttttgatcgc ctgcgcttcg tcgagagact tccctttcac
    29941 ccattcggtg accagggagc tggaagcgat agcagaaccg cagccgtaag ttttaaaacg
    30001 cgcgtcttca atgatacctt catcgttgac tttaatctgc aacttcatca cgtcgccaca
    30061 ggccggtgcc cccaccatgc cgctgccgac gttctcgtcg ttgttgtcaa aggaacccac
    30121 gttacgcgga ttctcgtaat ggtcgataac tttttcgctg taagccatta taaattctcc
    30181 tgattccgat accgattaat gatgagccca ttcgatgctg ttcagatcca cgccctgctt
    30241 gtacatttcc cacagcggag aaaggtcacg cagacgaccg atggatttac gaactaactc
    30301 gatggtgtag tcgatctctt cttcagtagt aaaacgacct aaagagaaac ggatagagct
    30361 atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg taggacggtt cgaggcttgc
    30421 tgacgtacag gcggaacctg aagaaactgc gaggtctttc agcgccataa tcagcgactc
    30481 accttcaacg tagttgaagc tgacgttgag aatgttcggc gcaccgtgtt ccaggtcacc
    30541 gttcaggtaa acttcttcga tatctttgat gccgttccat agacggttac gcaggccgcg
    30601 cagacgttcc atctcggtcg ccatctcttc ttttgcgatg cgataggctt cgcccatgcc
    30661 gacgatctgg tgaacaggca gagtgccgga acgcataccg cgttcgtgac cgccgccgtg
    30721 catttgcgct tcgatgcgta cgcgcggttt acgacgcaca tacagcgcac cgataccttt
    30781 cgggccatag attttgtgac cggagaaaga catcaggtca actttcaact ggctcaggtc
    30841 gataggcagt ttacccacgc tctgggttgc atcaacgtga tagataatgc cacgagcacg
    30901 gcacatttcg ccgatagccg cgatatcctg caccacgccg atttcgttat ttacgtgcat
    30961 gatggacacg aggatggtgt cgtcacgcat cgctgcttca agttctttca ggtcgataat
    31021 gccgttacgc tgcggtgcca ggtaggtgac ttcaaaacct tcgcgctcca gctgacggca
    31081 ggtatccagt accgctttgt gttcggtttt gctggtgatg atgtgcttgc cttttttctg
    31141 ataaaagttg gctgcacctt tgatcgccag gttgtcagat tcggttgcac cagaggtaaa
    31201 gacgatttca cgcggatcag cgccgaccag atcggcaatc tgattacggg cgatatctac
    31261 cgcttcttca gcctgccagc cgaaacggtg agaacgggag gccgggttac caaaggttcc
    31321 gtccatcgtc ataaactgca tcattttctc ggcaacacgc gggtccaccg gcgtggttgc
    31381 ggagtagtcg agataaatcg gtaatttcat tgctctataa actccgtaca tcactcaatg
    31441 caaggaatca ggctaccggc tggatgtacg accgtgttta cggagtattt agcactccgg
    31501 cctgattctg aattcttttt attaagcgcg taacttaacg tcgatcgcgt cttgtgtgcg
    31561 ggtgcgtggc gcgtcgtgag tatgctgacg accagacaca tccagcactt cctggttatt
    31621 aaccagttcg cctaaagtaa tgttgttgag aaaaccggtg agacggtcgc tcaaatcacg
    31681 ccacagcgcg tgggtcaggc atttatcgcc gccctggcag ccgcctttac cctgacaacg
    31741 ggtggcatct acagattcgt caacggcgct aattacttcg ccaacggcga tgctgctggc
    31801 atctttgcct aacagataac caccgcctgg tccacgtacg ctggaaacca gaccattttt
    31861 acgcagacgg gaaaacagtt gttccagata agaaagggaa attccctgac gttcggaaat
    31921 atcagccaac ggtaccgggc ccgcttcaga gttgagcgca acgtcaagca ttgcggtcac
    31981 ggcatagcgc cctttagatg tcagtctcat gtcttacttc acctcaaact cgcccctgcc
    32041 cggggttttt tattgtaaaa gtgggggtat tgcatagcag ggtcaagtct gacattcccg
    32101 agtaaattgg tcaactattt acttgactga tttagtcggg tatttaacct tcagtgccat
    32161 ttttttatcg tggcgtttgt acctgttgtc cttccagacc atgggcgggt tgagggcaat
    32221 tattcgcatt agcgctggcc tcttcccctc accctaaccc tctccccgaa ggggcgaggg
    32281 gaccgtccgt gcatttatct ggcttagccc tcaggcctta ttacctttat tctgctgctc
    32341 aatagaagcc agaatcccgc gcaggatatt caattcctgg ctttccgggc gcgcacgggt
    32401 aaacagacgg cgcaatttat tcatcacctg ccccggatgg ttttcacgga taaaaccggt
    32461 tgccagcagc gtttgttcca gatgaccata aaaacgctcc agatcatcga ccagcggata
    32521 cggcgtctct tcatgctcga cctgctcgcc gttttcctga gttgccagcc aggccatgcg
    32581 cacttcatag gcaataacct gaaccgccat cgccaggttc agcgagctgt attccgggtt
    32641 agccgcaatc gcgacatgat aatggcattt ctgcaactct tcattggtca agccgacgcg
    32701 ctcacgacca aacaccagcg ccaccggggt atttgccgct tcagcgacgc ttttcagacc
    32761 gcattcgcgc gggtcgagca tcggccacgg cagcgtgcgg gagcgtgcgc tggtgcccac
    32821 caccaggcta caaccggcta acgcttcgtc gagcgtatcg acgatgtgag cattaccaat
    32881 cacatcgctg gcccctgctg ccagggcaat cgcctgggag tcgggtttca ccagtggatt
    32941 aaccagccac agattggtta atcccattgt tttcatggca cgggcaacag aacccatatt
    33001 gccggtgtgt gacgtctcca ccagcacaat tcgaatattt tgcagcattg tctttcttca
    33061 tctaaagatt attcacgcat cttatcataa aacgaagaca gatgccgatc tcgctgctat
    33121 actctgcgcc gttttcccgt tctttaacat ccagtgagag agaccgatgc atccgatgct
    33181 gaacatcgcc gtgcgcgcag cgcgcaaggc gggtaattta attgccaaaa actatgaaac
    33241 cccggacgct gtagaagcga gccagaaagg cagtaacgat ttcgtgacca acgtagataa
    33301 agctgccgaa gcggtgatta tcgacacgat tcgtaaatct tacccacagc acaccatcat
    33361 caccgaagaa agcggtgaac ttgaaggtac tgatcaggat gttcaatggg ttatcgatcc
    33421 actggatggt actaccaact ttatcaaacg tctgccgcac ttcgcggtat ctatcgccgt
    33481 tcgtatcaaa ggccgcaccg aagttgctgt ggtatacgat cctatgcgta acgaactgtt
    33541 caccgccact cgcggtcagg gcgcacagct gaacggctac cgtctgcgcg gtagcaccgc
    33601 tcgcgatctc gacggtacca ttctggcgac cggcttcccg ttcaaagcaa aacagtacgc
    33661 cactacctac atcaacatcg tcggcaaact gttcaacgaa tgtgcagact tccgtcgtac
    33721 cggttctgcg gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg gtttctttga
    33781 aatcggtctg cgtccgtggg atttcgcggc aggcgagctg ctggttcgtg aagcgggcgg
    33841 catcgtcagc gacttcaccg gtggtcataa ctatatgctg accggtaaca tcgttgctgg
    33901 taacccacgt gttgtgaaag ccatgctggc gaacatgcgt gacgagttaa gcgacgctct
    33961 gaagcgttaa tgactcaggc gggtgatgtc actcacccgc cctcgccttt caggcgctat
    34021 tctgaaatac ttcctcaccg ctttactttc tttcgtcact ctcccaccat ctttccccga
    34081 ttaatggatg ataaagaagt cgcaaggaaa ggttatggca ctgccagtga acaaacgcgt
    34141 tcccaaaatt ctgtttattc tctttgttgt tgccttctgc gtttatttag tgccgcgcgt
    34201 tgccatcaac ttcttctatt atcctgacga taaaatttac ggtcccgacc cctggtcggc
    34261 ggaatccgtc gaatttacgg ctaaggacgg tactcgtctg caaggctggt ttattccttc
    34321 ttcgacgggc cctgctgaca acgccatcgc aaccatcatt catgctcacg gcaatgccgg
    34381 aaatatgtcc gcccactggc cgctggtcag ttggttacct gagcgtaatt tcaacgtttt
    34441 tatgtttgat tatcgcgggt ttggtaaatc aaaaggcacg ccgtcccaag ccggattgct
    34501 ggacgatacg caaagtgcca tcaatgtggt gcgccatcgc agtgatgtaa acccacaacg
    34561 gctggtgctg ttcgggcaga gtattggcgg ggcgaatatt cttgctgtta ttggtcaggg
    34621 tgatcgtgaa ggcatacgtg cggtgatcct cgactccaca tttgcctctt atgcaaccat
    34681 cgccaaccag atgatccccg gcagtggcta cttacttgat gagagttaca gcggcgaaaa
    34741 ttatatcgcc agcgtcagcc cgatcccgct tttactcatt cacggtaaag ctgatcacgt
    34801 tatcccatgg cagcacagcg aaaagttgta tagcctggca aaagagccaa aacggttgat
    34861 cctaatcccg gatggcgaac acattgatgc tttttccgat cgtcacggcg atgtttatcg
    34921 cgaacagatg gtggacttta tccttagcgc gttgaatcca cagaactaac ccatgatcgc
    34981 tagcacgata atcattcaca aaaccacctt aagacatgct aatccactgg tcagaacagt
    35041 ttaagatgag aaaaattctg tgacgcttgc caacatttct gatgattagc attcccttcg
    35101 ccatttccct gagcaaactt tagctattct tatcaattat gcttatggga gatctacaga
    35161 tgatgcctac gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc tgccagatct
    35221 tgctgacgct ctttcagccg gggttgaccg ccaccacggc aactttcagc gagcttaatg
    35281 gtgtggatga tgatattgcc agtcttgata tcagcgaaac aggacaggag atcctgcgct
    35341 atcatcaact cacactgacg gctggttatg acggtagcta ccgggttgaa ggtacagtgc
    35401 ttaaccaacg tttgtgttta tttcactggc tacgacgtgg tttccgtctg tgtccatcat
    35461 ttattaccag ccatttcacc cccgccctga agagtgaact gaagcggcgc ggaattgcgc
    35521 gtaactttta cgacgatacc aatctacaag cgttagtgaa tctctgctcc cgacgactgc
    35581 aaaaacgctt tgaaacgcgc gatattcatt tcctgtgtct gtatctgcaa tattgtttgc
    35641 tgcaacacca cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc tgggcggaat
    35701 cctgccttga atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt cgggcgctcc
    35761 agcctgtacc gcctgatgag ccactgttta tggcattact tttttccatg ttgcgggttc
    35821 ccgatccact gcgggatgcg catcagcgag acagacaatt gcgtcagtct atcaaacgtc
    35881 tggtaaacca ttttcgtgag ctgggaaatg ttcgttttta tgatgaacag gggttatgcg
    35941 atcagcttta tacccacctc gcccaggcgt taaatcgcag tttgtttgcc atcggtattg
    36001 ataataccct gccggaagag ttcgccagac tgtacccacg cctggtgcgc accacccgcg
    36061 cggcgctggc cggatttgaa agtgaatacg gcgtccatct ttctgatgag gaaagtggtc
    36121 tggtcgcggt gattttcggt gcctggctaa tgcaggaaaa cgatctgcat gaaaaacaga
    36181 ttattctact gaccgggaat gatagcgagc gagaagcgca gattgagcag cagttacgcg
    36241 aactaacgtt actgccgctc aacattaagc atatgtcggt aaaggcattt ttgcagacag
    36301 gcgctccgcg tggcgcggca ctgattattg cgccttatac catgccatta ccgctctttt
    36361 caccaccgct gatctatacg gacctgacgc tgacaacaca tcaacaggag cagatccgca
    36421 aaatgctcga atcagcatga gggaacaact ttcgggcgca aaaacattgc cggcagcgcc
    36481 accagcgcca ttacccagaa cacgccgtgg cccagatatt gatacaggaa accggcgaaa
    36541 acggtcatga tagcgatact gccgcccatc gcgacggcag agtaaaccgc ctgtaaacgg
    36601 atgacttcgc taccctggcg agcagcaata taacgcatgg cggccaggtg gcagaccgtg
    36661 aaggtgccgc aatgcagaat ttgcaccact atcaaccacg gcaacgccgt agttgctccc
    36721 ataatgcccc agcgcactac gccgcaaatc gccgagatca acagcatatc gcgtgcacta
    36781 caacggcgga aaagtttatt actcagcgca aagataatga cttccgccac cacgcccagc
    36841 gaccacaaat accccaccgc cgaggccgag tagccagctg cctgccagta aatggcgcta
    36901 aaaccgtaat aggccgcatg tgccccctgc aataaacaaa cgcaggccag aaagcgccag
    36961 ttctggcgaa ccagcgccaa ccacgctgac caaccggtgc tctcctgctg gcggcttgcc
    37021 ccttgtggct gaatcgtcgg acggatgaga aagccgagca gcatggatgc cacgcccaac
    37081 gtcaacagcg cgaggatcac ccgataatca aacatagtga ccagtttgcc cgtcagcgcc
    37141 gagccaatga caaacgccac cgagccccac agtcgcactt tgccgtaatc aagcgggaac
    37201 tgcttttgcc acgtattcgc cagtgcatcg gtcaacggta ccagcggtga gaaaaagagg
    37261 ttaaagccaa tcatcaccag catcagccac gctacgtgcg cccccgccca gaaggcgaca
    37321 gcaaagagaa gtgtcagcag tgccagcacg cgcaaggcgg aaatcaggcg ggaaggatcg
    37381 ctgacgcggg gcgcgatgag caaactcccg agaaaacggg caaccagacc tgcccccaat
    37441 aacaggccga tggtttctgg cgttaaacca atccctttaa gccagacgct ccagaaaggt
    37501 agaaaaatgc cgtaactaaa aaagtatgtg aaatagccga gcgccaacca gcgcgtggat
    37561 tgcaaaacca tgagtccctc ccgtcaggag gcgttagtct ggggttaatc gaatgttggt
    37621 gcaagttgaa aaataagaat attcgagcct gcttcccgat gtctgtaacg ccagttcaca
    37681 agcactaaaa aactgagatc cgcgacgcat gttgcaaaaa taatgcgtaa ttatgccgtt
    37741 acgcttgcca aacgttcctg cacaatggcg atgaaatcgc gtaatgccgg cttcatctcc
    37801 cctttcttcc acgccatcag taaagcaatg gaaggcacat ttccggcaat gggacgaaaa
    37861 acaacctgtc cggtattaaa attattcata taaccgggta tcaaagtgac gcccagcccc
    37921 atgcccacca gattcatggt caccagaata ttcgttgcca cctggacgat atttggctgg
    37981 ctgttttctt gcgcaaacca cgctttaacg atcggcgcaa gcgaaccgga atacgccgga
    38041 tcggtactga cgaaattcac gccatccagt tgcgcggcgg tgatctcttt ttcatgtgct
    38101 aaagggtgat caaccggtaa cacaaccact aatggttcgt caaaaagctc cagataatca
    38161 atctccgggc tataaacagg atggcgcatt aagccgacgt cgagttcacc acggcgaatt
    38221 ttttcctcct gttgcgtggt gattaaactc accagctcaa tcaaggtgtc tggctgtctg
    38281 agacgaaaca tcggtaatac ttttggcagt aaattcactt ccgccgatgg cacaaagcca
    38341 atggttaatt gtctgtcttc ctgaacaatt ttccgcgccc gtaatttggc attttccgct
    38401 tgttcgagga ttgccagcgc gtcctggaga aaacattctc ccgccgctgt caacgcgact
    38461 ttgcgcttat ccctcaccag taacggaaca ccgacacagt tttcaagatc gcggatctgg
    38521 ctgcttaacg aaggctgtga ggtatgcagt ttttccgccg cacgggtaaa gttcagtgcc
    38581 tgcgccactg cgacgaaata gcgtaaatgc cgtagttcca tcaccttccc cttgttatcg
    38641 aaaaaacgtc tcagctggta gaaattaact atttcacata ttagcaacca accagcaaca
    38701 tccttatggc acaaaaatag aaggtcaata catcttatct ttcaggatta aaaaatatga
    38761 ccacaccctc agatttgaac atttaccaac tgattgacac ccaaaatggt cgggtcactc
    38821 cgcgtattta taccgacccg gatatttacc aactggagct tgagcgtatt ttcggtcgtt
    38881 gctggctatt tctcgcccac gaaagccaga tcccaaaacc cggtgatttc tttaacacct
    38941 acatgggaga agatgcggtt gtcgtagtgc gtcagaaaga cggcagcatc aaggcgtttc
    39001 tcaaccaatg ccgccaccgg gccatgcgtg tgagttatgc agattgcggc aacactcgcg
    39061 cctttacctg cccgtatcac ggctggtctt atggcattaa cggcgagttg atcgatgtac
    39121 cgctggaacc tcgcgcctac ccacaagggt tgtgtaaatc ccactgggga ctaaacgaag
    39181 ttccttgtgt ggagagttat aaagggctga tttttggcaa ctgggatacc agcgcaccgg
    39241 gcctgcgtga ttacctgggt gacattgcct ggtatctgga tggcatgctg gatcgtcgcg
    39301 aaggcggcac cgaaattgtc ggcggcgtac aaaagtgggt gatcaactgt aactggaaat
    39361 tcccggcaga gcagttcgcc agtgaccagt atcatgctct gttcagccat gcttctgccg
    39421 ttcaggtatt aggggcgaaa gatgatggca gcgataagcg cctcggtgat ggacaaaccg
    39481 cccgcccggt gtgggaaacc gccaaagatg cgctgcaatt tggtcaggac ggtcacggta
    39541 gcggtttctt ctttactgaa aaaccggatg ctaatgtctg ggtcgatggc gcagtttcaa
    39601 gctattaccg cgaaacctat gccgaagcag aacaacgttt aggtgaagtt cgcgccctgc
    39661 gcctggcggg tcataacaat atattcccca cgctttcatg gctaaacggc actgccacac
    39721 tccgcgtctg gcatccgcgc ggccctgatc aagttgaagt gtgggcgttc tgtattactg
    39781 acaaagccgc ctccgatgaa gttaaagccg cttttgaaaa cagcgccact cgtgcttttg
    39841 gtcctgctgg ttttctcgag caggatgact cggagaactg gtgtgaaatc cagaaattgc
    39901 tgaaaggtca ccgcgcccgc aacagcaaac tgtgtctgga aatggggctt ggtcaggaaa
    39961 agcgtcgcga cgacggcatt ccaggcatta ctaactatat cttctcagaa acggccgctc
    40021 gtggaatgta ccaacgctgg gccgatcttc tgagtagcga aagctggcag gaagtgctcg
    40081 ataaaaccgc cgcttaccag caggaggtga tgaaatgagt gcgcaagttt cactagagtt
    40141 acatcaccgc attagccagt ttctctttca cgaagccagc ttactggacg actggaaatt
    40201 tcgtgactgg ctggcgcagc tcgacgaaga gattcgttac accatgcgca ccacagttaa
    40261 cgcgcaaaca cgcgaccgcc gcaaaggcgt ccagccaccg acaacctgga tttttaatga
    40321 caccaaagac cagctggagc ggcgaatcgc ccgtctggaa acgggcatgg catgggcaga
    40381 agagccgccg tcacgcaccc gccacttaat cagcaactgc cagataagcg aaaccgacat
    40441 cccaaacgta tttgctgttc gggtgaatta tctgctttat cgggcacaaa aagagcgcga
    40501 tgaaacgttc tatgtcggga ctcgttttga caaagttcgc cgtctggaag atgacaactg
    40561 gcgcttgctg gaacgggata tcgtcctgga tcaagcggta atcacttccc ataacctgag
    40621 tgtactgttc tgatgaatcg aatttatgcg tgtcccgttg cggatgtgcc ggagggtgag
    40681 gctctccgga tcgatacctc gcccgtcatc gccctgttca acgttggcgg cgagttttat
    40741 gccattaacg atcgttgcag ccatggtaat gcgtcaatgt cagaagggta tctggaagat
    40801 gacgccacgg tggagtgccc gctacacgcc gccagttttt gcctgaaaac ggggaaagcg
    40861 ttatgcctgc ccgccaccga tccgctcacc acttatccag tacacgttga aggtggtgac
    40921 attttcatcg acttaccgga ggcgcagcca tgagcgatct gcataacgag tccattttta
    40981 ttaccggcgg cggatcggga ttagggctgg cgctggtcga gcgatttatc gaagaaggcg
    41041 cgcaggttgc cacgctggaa ctgtcggcgg caaaagtcgc cagtctgcgt cagcgatttg
    41101 gcgaacatat tctggcggtg gaaggtaacg tgacctgtta tgccgattat caacgcgcgg
    41161 tcgatcagat cctgactcgt tccggcaagc tggattgttt tatcggcaat gcaggcatct
    41221 gggatcacaa tgcctcactg gttaatactc ccgcagagac gctcgaaacc ggcttccacg
    41281 agctgtttaa cgtcaatgtt ctcggttacc tgctgggcgc aaaagcctgc gctccggcgt
    41341 taatcgccag tgaaggcagc atgattttca cactgtcaaa tgccgcctgg tatcctggcg
    41401 gcggtggccc gctgtacacc gccagtaaac atgccgcaac cggacttatt cgccaactgg
    41461 cttatgaact ggcaccgaaa gtgcgggtga atggcgtcgg cccgtgtggt atggccagcg
    41521 acctgcgcgg cccacaggcg ctcgggcaaa gtgaaacctc gataatgcag tctctgacgc
    41581 cggagaaaat tgccgccatt ttaccgctgc aatttttccc gcaaccggcg gattttacgg
    41641 ggccgtatgt gatgttggca tcgcggcgca ataatcgcgc attaagcggt gtgatgatca
    41701 acgctgatgc gggtttagcg attcgcggca ttcgccacgt agcggctggg ctggatcttt
    41761 aaggaagcac gatgaaagaa aaaacgatca ttattgtcgg tggcgggcaa gcggcggcaa
    41821 tggctgcggc ctcgctacgc cagcaagggt ttaccggtga gctgcatctg ttttccgatg
    41881 agcgacatct tccttatgaa cgacctccac tctcgaaatc catgttgctg gaagattccc
    41941 cgcagttaca gcaggtgtta cccgctaact ggtggcagga aaacaatgtt catctgcatt
    42001 ccggtgtaac catcaaaacg ctgggtcgcg acacacgaga gttagtgtta accaacggcg
    42061 aaagctggca ctgggatcag ctttttatag caaccggcgc ggcagctcga ccgctgccgt
    42121 tgcttgatgc actgggagaa cgctgcttta ccctacgcca tgccggtgat gccgccagac
    42181 tgcgagaagt tctgcagccc gaacggtcag tcgtgattgt cggtgccgga actattggtc
    42241 tggaactggc tgccagcgcc acgcagcgca gatgtaaggt gacagtgatt gaactggcgg
    42301 caaccgtcat gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta cagcgccacc
    42361 agcaggctgg tgtgcgcatt ctgctcaata atgccattga acatgtggtc gatggtgaaa
    42421 aagtagaact gacgctgcaa agtggggaaa cgcttcaggc tgatgtggtg atttacggta
    42481 ttggtatcag cgccaacgag caactggctc gcgaggccaa ccttgatact gccaatggca
    42541 ttgtcattga tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg
    42601 caatcactcg tcttgataat ggtgcactac accgctgcga aagctgggaa aacgccaata
    42661 accaggcgca aattgccgct gccgcaatgt tagggctacc gctaccgcta ctgccgccgc
    42721 cgtggttctg gagcgatcag tacagtgata acttacagtt tattggcgat atgcgtggcg
    42781 atgactggct ttgtcgtggc aacccggaaa ctcagaaggc gatttggttt aatctgcaaa
    42841 acggcgtgct tatcggtgcg gtcacgctga atcaggggcg tgagattcgc ccaattcgca
    42901 aatggatcca gagcggcaaa acgtttgatg cgaaactgct gatagatgag aacatcgcgc
    42961 ttaaatcact gtaaccagga taattagcga atatctcaat gcctggggcg tggcgaggtg
    43021 caagagtgtg tattacgttt aaatcacatt atcttgcaaa gggattggtt atgaacacac
    43081 tacgttattt tgattttgga gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg
    43141 tggtcttaat tttcattatt tttggttttc ccaaaatgat gggctttgac ggtacggtcc
    43201 aatatatggc ctcgttgggc gcgccaatgc cgatgctggc agcgattatt gcggtagtta
    43261 tggaagtgcc cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg ctggcggtgc
    43321 tgtttatttt ctacacgctg ggtacggcgg tgattggtca ccattactgg gatatgaccg
    43381 gcgatgcggt tgggccaaat atgattaatt tctggaagaa tgtcagtatc gctggcgcat
    43441 tcctgctgct ggcaattacc gggccggggg caatttctct cgatcggcgt taggaaagat
    43501 gccggatgcg gcgtgaacgc cttatccggc atttaataaa ttacagccac tctacccgca
    43561 acgacatctc tgaagttgtt gattcccctg gtgctagcgc aatgagatca cccccttccg
    43621 ggcgatgatg atcatccggc gcatggctca tcggttccag acagaagaaa tcaaacgcat
    43681 atcctttatc aaacgcaggg tcggaaacaa agatgaaata acacggtgca ggtggcgtcg
    43741 tttccatgat gatggcataa ccttcctgcg gctgctcgat gcgggcctga ccattccatc
    43801 cggcaaaacc attgttcacc cactggcgcg gcaacggcgc gggctggtta aaatccagtt
    43861 cctgcggtag ctgctcgcaa aactcacccg ccagccactg ctcccgctcc agccagtaac
    43921 cgctcgcctg tgcctgaatc cgcgtttgcg gcgacaacgg aaaataagga tgccagccgg
    43981 taccaaatgg cagcgtctct gccccttgat tggtgacaga gagcgtcacc gtcagcgtat
    44041 ccgccgttaa atgaaacgcc tgacttaccc gatagtgata gacaccgctg cgatgttcat
    44101 acaccagaca caaactatca tcgctgtgcg agacacattg ccactcgccc agccagccat
    44161 cgccgtgtag atagtgcgca tcccactcaa cattcggttg cagttgatac tcacgcccct
    44221 gccagacaaa acgattgccg ctcacccggt ttgcaaatgg caccagcgga aaacatgagg
    44281 catcggttgc cacaccgctt tttttaccag gacgtaagag cggcgtcgta tcgcgccaga
    44341 agccttcgat tacgccgccc tggtcagaaa cgtccagctt tagcgacccg tgggataagg
    44401 tatagatggt catccgctaa ctccttaatc cgggaagtta atcacaactt tcccgcattt
    44461 gccgctcgcc atcagcgcat aggcatcacc tgcctgttcc agcgagaagc gatgggtaat
    44521 ggcgttacgc ggccacagct tccagtccgt cagatcatgg gcgcattttt ccatatggaa
    44581 cagactggtc acccaggagc cgataatccg ccgttgatgg tgcatcagat cggcgctgac
    44641 ctcgaattcc acttttccgg tttcaccaat gtaaaccacc cgtccccaat cagcggtgga
    44701 ttgcagtgcc agcaagcgac ctgcggcatt accggaacaa tcgagcgcaa catccgcgcc
    44761 accgtgggtg agttcggcga taatctgcgg cagaccttcg gtggttgcta aatagccgtg
    44821 atccatcacc cctaactgtt ttgccatcgc cagacgttcc ggcagcatat caacgccgat
    44881 gatccgtttt gcaccgcgac ctttcgccag catcatcgcc atcatgccga ccggccccag
    44941 accgaccacc agcactttat cgctgccgga aacttcgccg cgcaaaatcc cttcataggc
    45001 cgtgccaacg ccgcaactga taaacgcacc atcttcgtag ctcagcgcat ccggcaggag
    45061 gatcagatct ttttcttccg ccagcagata ttcggcatga ccgccgtcac gctgccagcc
    45121 gtaagccgct tttcctttgc cagtacaaga gataggaaag ccgcggcggc agttcgggca
    45181 aaaaccacag ccagaaatgt gatacaccag cacgcgatca ccctctttaa aatggcgaca
    45241 gccttgcccc atcgccacaa tctgcccgca cggttcatga ccgttgataa atccctggta
    45301 taacggttta tcgggtgccg ccgccgtggc acggtgttga tgatagatat agtggacatc
    45361 gcttccgcaa atcccggagg atttcatttt gatcagtacc tggttaatcc ccggcgtcgg
    45421 caccgcaact tcccgcagat cgacggtcga atttcctggt aaataagctg ccagcatcgt
    45481 tttcataaat ccctcattaa caatacgatt aattttcatc cctgccgcac ccgcgccagg
    45541 ggcgttaagg tttagcgttt cgctttactg ctacgctggg tcagcaagat attcgccagc
    45601 accgccacca cgatgatgac gccgcgcacc acctgctgga aaaaggagtt aatcccgagc
    45661 agcaccagac cgttaccgat tagcgtaatc accagcacac caagcaatgt accgaacagg
    45721 gaaccgcgac cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac
    45781 tccagaccgt ttgcggcacc tgcgttacca gaaccgaggc gcgccgccaa caaaatgccg
    45841 gtcaccgccg ctaataatcc cgaaagggta aagataagaa tgcgcacccg acgaacgttg
    45901 atgccgcaca actgcgccgc cgtagcatta ccgccaacgg caaaaaccga gcgcccgaag
    45961 gcggttttgc ggctgatgaa cacaaacagc gcaaacaaca caatcatgat cagcgcggat
    46021 accggcacac cgagaaattg tccgcccagc cagtccagca cctcgttttc gtcaatcggc
    46081 actggcagtg cgttcgtcat aaacagcccc attccgcgca gggcgctcca cagccccagt
    46141 gtggcaacga aacttggtac gttaaacacg ccgcgcagca ccccggccag cgttcccatc
    46201 agcgcgccta acagcaacac cagcagacac gccaccgcca gcggaacttc aaattgcagc
    46261 aaaaatgcca ggcacaccga gacaaaagcc accatcggcc caacgctgac atcaatctca
    46321 ccggagataa taatcagcgt catcgcccag gcggcaatcc caatggtggc ggcatcgcgc
    46381 agcacgttca tctggttatt caatgagata aagccaggcg cgttcaggga gaagaccaga
    46441 taaagaatgg caatcaccac cagcaaaccg atctcattaa tatggcgact gacaaattgt
    46501 ttgagcgaga cgctcttgcc ctgcggcaat ggtaatgacg aagcagacat cttcaggttc
    46561 ctcggtaaaa ttcatcagtg cacagacaga atggcggaca tcagctcatc cacattgacc
    46621 ggcgagtgaa actcctgcga gaacgtgccg tgctgtaata acaggatgcg gtcacacacc
    46681 agcggcaact cctccacttc actggagata aacaccacgc tttttccttc ggcagccagc
    46741 tcacggacaa tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg
    46801 tcgagcaaca aaatctggct ggcggcatag acccaacgac cgatcactac tttttgctga
    46861 ttgccgccag aaagcgtgcc gatgggcgtt tcgctactgg cggccttgac cgtcatccgc
    46921 tgcatcactt cttcggtcag gcggcggatg gtggaccatt gcagcactcc gttggcgctg
    46981 attttttgcc gattggtcag cactgtattt tcgtcaacgc ccaaccaggg aatgatcccc
    47041 gcttctttgc ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg
    47101 gggcgcgtga ttttctcgcc gttgataaca atttcgccct gttcatactc ctccagccca
    47161 acaatcgcct tcagcaattc actgcgccct gcccccagca gaccagcaat gccgagcact
    47221 tcgccacgac gtagcgtaaa actgatatcc tccagcttgg gcttatggcg taacgcacgg
    47281 acttccagta cggcctgatc cacaatttcc tgaggggcaa ccggcgcaat atcaacgtga
    47341 tcgcgcccga gcatcagcga cacaatatga tgcgtggagg tgttttcgag catcacatcg
    47401 cccgccacct gaccatcgcg cataacggtg gcacaggagg caatgcggcg aatttcttcc
    47461 atccggtggc tgacataaat taccgccacg cccagtgccg acatcttttt caccgcgctg
    47521 atcaccagtt caacttccgc actggcaagc gaactggtag gttcatcaag aatgaccacg
    47581 cgcggctcgc ccttcatcac ccgcgcaatt tccaccagct gcttttgcgc cgggcttagt
    47641 gttgaaacaa gttgttcagg actaacgtca acgcccagcg cctgtaagca acgttgggca
    47701 tcctgcgcca tttgcaggta atcaatcatg ccgttgcggc ggggccactg accgaggcag
    47761 aggttttccg ccaccgtcag cccttccacc agacttaact cctgataaac cgcgcgtacc
    47821 cccagttcag cggcacggcg agtcagcgta gcttcgtcgc cttccagtcg cgtctcgcca
    47881 atccagatat caccgctatc cggacgttcg ctaccggtaa gcattcgaat gagggtcgat
    47941 ttgcccgcgc cgtttttgcc taacagcgca cgaacttcgc ctttattgag cgtgaagtta
    48001 acgttatcca gcgcaacgac gccggggtaa cgcttatttc ctgccaccac ttttgctacc
    48061 gggactgcct ctgttgccgt gaacataggg acctctgcga atcagcgatt agggcagacc
    48121 atcaacgtgc gttgccagcc actgtttacc gtcttccgtt ttggtataga gatcgattgg
    48181 cacctgaatc actttttcac cgtcggcttg tttattgata accttcaatg tttgcgcgaa
    48241 aacagcattg cccattttct taccggaaat atccaccacc gctttcaaca cctgattgtt
    48301 ttccagctcc tgagcaattt cagtggtcat atccgaaccg aaaacagcaa tttttccggc
    48361 ctgattttga ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg attcccccat
    48421 aatggcgttg agatccggcg tggagataat tagtttttca ccaacggaaa tcgctttatc
    48481 taaaacagtc ccttcctgat tagcgacaat ttgcgcgccg ggaacgcggg attttaatac
    48541 ttcttcaaat cctttacgtc gctgcacaca aacttcaaag gcttcgcaat tgatgacggc
    48601 aattttcggc tggtcaattt tattggcaat aaaataatcg gcggcagcgt tacccagttt
    48661 tttaccaaat tccagcggat cgccgaccag atacgccgag acatatttat cgaccccctt
    48721 ttgattaata caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac
    48781 ggtactgcta ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc
    48841 cacgagggta tcaacaaagg tactttcttt cgaaatatca ccctgggcgt tagtttcaat
    48901 taactgtacc tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc
    48961 gtaatatccc tgggtatcaa ggtatattgc accaatggtc atttctttat ccgcagccct
    49021 ggcaaatagt gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat
    49081 ttttttaggc atagtgcctc ctgtagggtt tttattaaca acggcttatt ctaattattt
    49141 tgtgatgagc ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta
    49201 gttaaaaata aaaggcatca cggtggtgaa taatgccgct ttcggccagg ccggattaat
    49261 cgccgtcaat tcagcgcgag cagattcata ttgcgcaacc tccccgagtc ccgcggacgc
    49321 cagcatcgcc accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga
    49381 caacaggtca ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc
    49441 ttgcgcccac tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt cacccagcag
    49501 tcgcagcgcc attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg
    49561 gatgttaatg gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc
    49621 atcgccctgc gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc
    49681 cggtaaacgg ccttcgctta aattctccgg ataatgcagc gcggcatgaa gcagttcgct
    49741 ggcctgctgc ggctgtctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat
    49801 aaactgactg gtgaccttcc cttccccgcc ttcccacggg tggaatttgc gcgtggcgag
    49861 aatgtccgcc gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt
    49921 catgtcgtcg cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc
    49981 cggtgtggct ccacttaact tatccagcaa atcccgttcg aaaagcagac gtgcatcctg
    50041 cggcgcaagc tgataagcat tatcaagata acgcgcggcc agttcataat cgtgttgctt
    50101 attccacgca tggatcgcca acccgcgcca gccatcggca aactccggcg acatctctac
    50161 gcaactttgc cataaagtaa tggctttgtt gtagctgcgc ttgttgtagt agaagcaggc
    50221 cagtaaatga cgggcaaacc agcactcttc aatactctcc agcgccgcca cttcttccag
    50281 cgtattcggg aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc
    50341 gcgttcggct ttcggcagca agctggcttg tagataaagc gataacgtgc gctgacagtc
    50401 cagtgcgttc agcatctctg tcgccagagt gggcattccc cagttaatca gttgcccggc
    50461 ggtcatcagg gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagagctga
    50521 ttcgctacga ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcgcg
    50581 cagcagtttc tcgcgctgca cacgcgcgtt gtcctgacga ccacttaaca ccagcagcag
    50641 attatgcagg caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa
    50701 atccagacca gcgtcgaagt taccattacg cgccgccagt cgtgccaggc cataatagcc
    50761 accggctttg ctgttgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata
    50821 ttgtccctga cgttcgtaag cactggcgcg aatcaaactg gcctgtccgc actgcggatt
    50881 tttgttcagc gcatgtgcgc gtttcagcgc ctgagtggca tacgccaccg cttgcgggaa
    50941 atctgcgcgg ttatattcca gcatcgccag cgccaggtta cagcggtaat ccagcggatc
    51001 cagcaccacg ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc
    51061 cagatgctga ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgcgagtgg
    51121 tgctttggcg acgtccggca acggcaactc ttgcggctga tgttcctgat aactcaggac
    51181 aatattgccg tcggcatcgg agagctcaat ggtcagcctt tcaggattga taccgtgcaa
    51241 cacgccctgg atggcggtcg caggcatcag tgccacggca tcatcaagta acgcgttgca
    51301 tttgccgatt tcgcggatcg ccaggcggta tccgttcaac ggagagatgg catacagccc
    51361 ccactcaatc ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg
    51421 caccatgccc aaagaatgat aaggcaggaa atactgctca aaacgcttct cttcgtaagc
    51481 atcaagccag gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata
    51541 cgggccgtta ttgtcggtca gactcttgtc ccacgcctgg ccgaattcac tgtgtcccca
    51601 actccactgt tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc
    51661 ttcatcgtga caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt
    51721 tggaacaggc acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta
    51781 gtaagtgccg gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc
    51841 agtcacatcc ggtgggaaaa cgctctgatg cccttccccc cctttcactg ccgggttggc
    51901 ccaccacaag aaatgacgcg gcgtggcgtt accgttatag acgcggctgg cgatttccag
    51961 cgccgcccgg tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc
    52021 cgtttcgcct acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac
    52081 gggcataaag gtggtcgggc gatgatgttg cggccagtta aactcaatcc caccggaaat
    52141 ccacggcccc agcagcccca ccagcgcagg tttaatgact tcattgtgat aaacaaaatc
    52201 gcgctgtttc actttatccc acgcgcgatg cacccgaccg cccagttccg gcaggatcat
    52261 cactttgatg tagtcgtttt ccagccacac cgcctgccag gatttcaggg ttttctgctc
    52321 gctcagcgta tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg
    52381 attttccagg aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac
    52441 gcgctcttgc cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga
    52501 attaaaagat ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg
    52561 caattaacta aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc
    52621 caccataaca aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca
    52681 acgctggccc ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg
    52741 gaaagcgaaa aacgggtggt gaatatcgac gatgaaagcc agacgcgcgg gcatagtagc
    52801 ggtacatggc tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc
    52861 agtattgagt gtcaggttgc taatgcttgt ttaagtccga aaggcgagtt tgagtattta
    52921 cagattgatg caccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc
    52981 caccgtaaat tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cggtcaggtt
    53041 gatccggtga ccggcgtgtc gcaaaccatg ccgcaagcgc cgtggacaac gccggtggag
    53101 gtgaaatatc tgctggaaga gaagctcggc attcgggtga tggtcgataa tgactgcgtg
    53161 atgctggcgc tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc
    53221 aacgttgatt acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt
    53281 ttgtatggta gcggacagat tggtcacacc atcgttaatc cggatggcgt cgtctgcgac
    53341 tgtggacgtt acggctgcct ggaaaccgtc gcctcgttaa gtgctttaaa aaaacaggcg
    53401 cgggtatggc taaaatcaca accggttaat actcagcttg atcctgaaaa actgacaacc
    53461 gcgcagttaa tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgaccgc
    53521 agtgccaatg ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag
    53581 atctggttgt acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc
    53641 cagacgggat ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt
    53701 ggcttcgggc aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag
    53761 gcgcagttac gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt
    53821 ttttcaacag attaaccgtt tcgcttatgc gtaaaccggg taacgtgcgc agatgtcgag
    53881 aactttacct ttgatgcgct cgataacggc ttcatcattg atgctgtcca gcacgtcaca
    53941 catccagcca gccagttctt tcgcttctgc ttctttgaag ccgcgacgcg taattgccgg
    54001 agtacccacg cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg gtacgctgtt
    54061 tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac cggtcaggtt
    54121 tttatcaacc agatcaacca ggaacaggtg gttatcagtg ccgccggaaa ccactttgta
    54181 gccgcgttcc aggaacactt ctaccatcgc tttagcgttt ttagcgacct gctgctggta
    54241 agttttgaac tcaggctcca tcgcttcttt cagagcaacc gctttaccgg cgattacgtg
    54301 catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt tgtacagctc
    54361 ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga cccgccaggg ttttgtgagt
    54421 ggtggtagta acaacgtgag catgaggaac cgggttcggg tagacgccag cagcaaccag
    54481 gcccgcaacg tgcgccatat caacgaacag gtaagcaccg atgctgtcag cgatttcacg
    54541 cattttcgcc cagtcaacca cgccggaata tgcagagaaa ccaccgataa tcattttcgg
    54601 cttgtgttct ttggcttgtt tttccagatc ggcgtagtcg atatgaccgg tagcatcgat
    54661 accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac cgtgagtcag
    54721 gtgaccgcca tgcgccaggt tcatacccag aacggtatca cctggttcca gcagcgcggt
    54781 gtagaccgca aagttagcct gggagccgga gtgcggctgg acgttagcgt agtcagcgcc
    54841 gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat actcgcaacc
    54901 gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct gagaaccctg
    54961 cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga tgtgctcttc
    55021 ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat cggcaatgtt
    55081 catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt tttggccttt
    55141 ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc gataacaggt
    55201 cttgacaaag gaatttacgc aaacgattac cttcaggcta cgcaaggctt tggagaataa
    55261 agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt ttcacttcat
    55321 cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt ttttataaga
    55381 tgcatttgag atacatcaat taagatgcaa aaaaaggaag accatatgct tgacgctcaa
    55441 accatcgcta cagtaaaagc caccatccct ttactggtgg aaacggggcc aaagttaacc
    55501 gcccatttct acgaccgtat gtttactcat aacccagaac tcaaagaaat ttttaacatg
    55561 agtaaccagc gtaatggcga tcaacgtgaa gcactgttta acgctattgc cgcctacgcc
    55621 agtaatattg aaaacctgcc tgcgctgctg ccagcggtag aaaaaatcgc gcagaagcac
    55681 accagcttcc agatcaaacc ggaacagtac aacatcgtcg gtgaacacct gttggcaacg
    55741 ctggacgaaa tgttcagccc ggggcaggaa gtgctggacg cgtggggtaa agcctatggt
    55801 gtactggcta atgtatttat caatcgcgag gcggaaatct ataacgaaaa cgccagcaaa
    55861 gccggtggtt gggaaggtac tcgcgatttc cgcattgtgg ctaaaacacc gcgcagcgcg
    55921 cttatcacca gcttcgaact ggagccggtc gacggtggcg cagtggcaga ataccgtccg
    55981 gggcaatatc tcggcgtctg gctgaagccg gaaggtttcc cgcatcagga aattcgtcag
    56041 tactctttga ctcgcaaacc ggatggcaaa ggctatcgta ttgcggtgaa acgcgaagag
    56101 ggtgggcagg tatccaactg gttgcacaat cacgccaatg ttggcgatgt cgtgaaactg
    56161 gtcgctccgg caggtgattt ctttatggct gtcgcagatg acacaccagt gacgttaatc
    56221 tctgccggtg ttggtcaaac gccaatgctg gcaatgctcg acacgctggc aaaagcaggc
    56281 cacacagcac aagtgaactg gttccatgcg gcagaaaatg gcgatgttca cgcctttgcc
    56341 gatgaagtta aggaactggg gcagtcactg ccgcgcttta ccgcgcacac ctggtatcgt
    56401 cagccgagcg aagccgatcg cgctaaaggt cagtttgata gcgaaggtct gatggatttg
    56461 agcaaactgg aaggtgcgtt cagcgatccg acaatgcagt tctatctctg cggcccggtt
    56521 ggcttcatgc agtttaccgc gaaacagtta gtggatctgg gcgtgaagca ggaaaacatt
    56581 cattacgaat gctttggccc gcataaggtg ctgtaatttg atgttgccgg atggaaacat
    56641 ccggcaaccc ttgacgcggt ttaaattgcc gcgtcgtcct cttcaccggt acggatgcga
    56701 atgacccgtg ccacgtcaaa gacgaagatt ttaccgtcac cgattttgcc ggtttgcgcc
    56761 gtgcgaataa tggtatcgac acaggtatcg acaatgtcgt ccggtacgac aatctcaatt
    56821 ttcactttcg gcagaaaatc caccatatac tccgcgccgc ggtacagctc ggtatggcct
    56881 ttctggcgac caaagccttt cacttcggtc accgtcatgc cggtaatacc gacttcggcc
    56941 agtgcttcgc ggacatcgtc cagcttgaag ggttttataa tcgcatcaat ctttttcatg
    57001 ctattccttg aaaaggtcgc ctgtcttttg atctgctaaa cgtaacacat aacgccaatt
    57061 cattccttga aatcgtttgc atccagctcg tgtcgggaaa gcagtttata aaattctgtc
    57121 cggttgcgcc ccgccattct cgccgcgtgg gtgacgttgc ctttggtaat ttgcagcagc
    57181 ttacgcaaat agttgagttc aaactgatta cgtgcctcaa caaaggttgg cagcgccgtg
    57241 ttttcaccct ccagcgcctg ctctactaat gcatcgctaa taaccggaga tgaggtcagc
    57301 gccacgcact gttcaatcac gttgaccaac tggcgcacat tacccggcca actagcggtc
    57361 atcaggcgtt tcatcgcatc agtggagaac gcgcggacaa acggtttatg tcgctctgcc
    57421 gcctggcgca acaggtgatt tgccagaagc ggaatgtctt ctgtgcgctc tgccagagcc
    57481 ggaattttca ggctgacaac gttgaggcgg taatagagat cttcgcggaa ttccccgcgc
    57541 gccatcgctt ttggcagatc acggtgagtg gcagaaataa tccgcacgtc gatatcaata
    57601 tcgcggttgc tgcccagcgg gcgcacttta cgctcctgca acacgcgcag cagtttgacc
    57661 tgtaacggcg caggcatatc gccaatttca tcgagaaata gcgttccgcc ttccgccgcc
    57721 tggaataaac cttcgcgatt gctgacagcg ccagtaaacg cgccacgcgc atgaccaaac
    57781 agctccgact ccagcaattg ctcgggcaat gcgccacagt taatagcaat aaatggtttg
    57841 ctattgcgcg ggctggcgtt gtggatagcc tgggcgaaaa tctctttccc ggtgccgctc
    57901 tggccgttaa tcaaaacgct aacgtctgat tgcgccacca gccgcgcctg ttccagcaaa
    57961 cgcagcatca gcgggctgcg ggtgacaatc gcctcgcgcc agcgttcatc ggtggctggc
    58021 gcggattgtt ccagcgcatc gtcaattgcc tgatatagcg catctttatc gacaggcttg
    58081 gtgaggaaac tgaacacgcc ctgctgtgtt gcagcaacgg catcgggaat agaaccatgc
    58141 gcggtaagaa taattactgg cattcccggc tgcactttct gaatttcagc aaacagctgc
    58201 ataccgtcca tttcatccat ccgcaggtcg ctgatgacta aatctacttt ttcgcgattc
    58261 agtacccgta atccttcagc gccactttcc gccgtgacca cgctgtagcc ttcgctggtc
    58321 aggcgcaggc caagcagttt cagcaatccc ggatcgtcat cgaccaataa taaatgcgca
    58381 ggtttatggc tcatcaggag tgacctcatg ggtggatggc gcgggctttt caccctcgtg
    58441 cggcgtatcc ggcgagaaat ttccagccgg tttgcgggtc gagagctggc gttcaatatc
    58501 agtcaggttt tccagcttgc gggtggtgag ttccagttgc tgctgtagaa cgtgatgttg
    58561 ctggcgcaat gtatccagct cgctgtcgct ggcctgctgg agcttgccgt agcgttggcg
    58621 ctcttccgcc agttgcagtt gcagcgcctg accgtcgcgc cagagttgat acagagggcg
    58681 aacctgtgcc gggatctcgg tacttagcgc atcaatgcgc gcgaccagct ggcggcgctc
    58741 atacggcgta attttggcgt cggcgagcaa aatcccttgt ttaaaggtat tttgccagct
    58801 gccgtcgtca tattggcgag cttgctgacg tgactgcgca ggcattaaac gatcagcaca
    58861 atccatcgcc cgcagccagt aaagcggatt ggtttcggtt gatttacctt gcagcgccca
    58921 gatgtcgcta cattcagtag aaagatagtc agccagttga taaaccggaa ttttttcttc
    58981 tgctggcgta tcaatggctg gcttattgtg actctgcacg caacccagca atgccagaca
    59041 tggcagccct gccagccaca gccgtcgggg caataatcgt tgaaaaatgt gtcgcatatt
    59101 caccagactt aatgcctatc ccagtgggcg taattattgc aggcagtttg gacatggaca
    59161 gcgcggagaa accggagcgt acacgtcgta cgtgaggatt tcgagcactg cccggggccg
    59221 aactggcaaa taaaatagcc tgatgggata ggctgttagc attatttcgt gtttttcgac
    59281 gacggcaatt caatgcggaa acaaacgtct tgcccgctct cgtcgaccag atacagttcc
    59341 ccttgcatac ggcgaataca atccctggca atgcttaatc ccagaccgct gcccttcacc
    59401 gccccttttc gctggtggct tccttgaaaa aagggttcga agatcatggc gcgttcctct
    59461 tgcggaatgg gtgtgcctgt attgatgaca tcaatataaa cccgtgcacc atgtaagctg
    59521 ctgcgaaggc aaatgttacc ggattcagcc ccgtagtgca ccgcattgga gtaaagatta
    59581 tccagtacgc tcatcagcag cattggctcc gccaggcaag ctgttgcttt gagatcgacg
    59641 tcggtatgca tcattttagc ccgtgcgggc aggctatgag cagaaactac tgtctccacc
    59701 agcggtgcta actcaacatt ctccagttcc accgcactgt ccgcttgttt acggttgtaa
    59761 tcaagcagtt gttcgatcag tttttgcaaa ttgcggctgc tgctatcaag aatgctcacc
    59821 acctcttttt gctctggcgt aagcggcccg acaacctggt cagccagtaa ttcagtgccc
    59881 tcgcgcatac tcgccagtgg cgtttttaat tcatgagata aatgtcttaa aaattgatgg
    59941 cgttgggatt ccagccatga caggcgctca cttaaccaaa gaatacgttg cccaaccgag
    60001 cgtaactcgc tcggtccact gaacgaaacg ctattgccca gagaacgccc ttcccccaga
    60061 cggttgatca tgcgctcgat atttttcacc ggcccgataa tcatccgcgt gaaaagtagc
    60121 accatcacca gactcaccag aaatagcacc agcgattgcc aaccaaaata ttgcccacgt
    60181 tcggcgattt cacgctgaag ttgctgccca cgagagaaca ccactgtgcg cgtggcctgc
    60241 accatttcgg tattggcact ggcaaaggct tcaagacgcg cggcggcggc agcatcggga
    60301 ccactgttgt tacactgaag ttgggccaga ttgttcaaat cctgacgcaa tgcctggtaa
    60361 agtttatcgt ctggcagtac gcctgcgtgg gcatcgagca tttcgctgta acgcttgcgc
    60421 tggctttgat aaaccttcgc aagcgtaggg tcgtccagca cgcaatactg acggtagcta
    60481 cgctccatct ccagcgccgc gttggtcatc gcttcactac gccgggcatc aataagcgta
    60541 gtgcggttaa ccagcgccgc ctgatcgcta agcgcattca ggctttgcca ggcttgccat
    60601 gccagcacca acaggggtag tagaattagc aaaaatgcca gcattaccag ttgtcgtaat
    60661 gagcggggaa aaacgggcca gcgtttcaag gtgttactct cgtcagacgc gaatagcctg
    60721 atgctaaccg aggggaagtt cagatacaac aaagccggga attacccggc tttgttatgg
    60781 aataaggcgg tgcctaactc gacgtttcgc ccgatggttg atatagctac gctgatatca
    60841 gaagttggac ggcaggcacc ttgttgtgcg tcattcgaat tttatgtagc acgtcccgaa
    60901 ggggctgaca taagtcggtg aatgagccac tgcttactat tatgcagtaa ctgtgccaat
    60961 aaagaaaata gtttggtaac gtactgatta tacgttgctt tgagggattt atggcttctt
    61021 cgctatttga atgtaaatca gccactgtgt cgctaaaaag agacaactta agataaactt
    61081 attagattac aataaaatca atcatttaag tgtcgccaga aagcgacacg gtaaaccacc
    61141 cattgtcgtg atttacagac acaaaaaagc tcccggagtt gggagcttat gatagtgatt
    61201 ggtgcttaat gccggatgcg gcgtaaatgc cgcatccggg ctgcaaaacc aatgaactga
    61261 tgacttaccc caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc
    61321 cccagttttc cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca
    61381 tcataatggt gactcgacca ctttcagtcg taaccgacgt aataccgttc ggagaaccgt
    61441 tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca
    61501 ccagcccttt gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt
    61561 caccatgaga gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagagacg
    61621 ggctttgggt cacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtattgcgca
    61681 caaaacgtgg ccacaactcg ctccccggga tcagttcacg caggttagac atcatctggc
    61741 aaccgttaca tacccccagc gccagcgttt gcggacggtg gaagaaggtt gcaaactcat
    61801 cgcgtacacg gtcattgaac aggattgact tcgcccaacc ttcaccggca cccagcacat
    61861 caccgtagga gaaaccaccg cacgcgacca gggcgtggaa atcttccagg cccgtgcgtc
    61921 cagccagcag gtcgctcata tgcacatcga ttgcatcgaa gcctgcacgg tggaaggctg
    61981 ccgccatttc aacatgtgag ttcacaccct gctcacgcag cacggcaact ttcggacgtg
    62041 cgccagtggc aatatacggt gctgccacat cttcgttgat atcgaacgac agtttcacat
    62101 tcaggcccgg atcggcgtcg tttgatttcg cctggtgctc ctgatcggca cactccgggt
    62161 tgtcacgcag gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtagtgc
    62221 ggctttcgct gaatacagtc tgcccgttgg cggtaatcac aaaacggtta ccggaaaccg
    62281 cctgccctac ataatggacg caatccgcaa gcccatgctg tgccagtacg gactcgaccg
    62341 cttcacggtc agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg
    62401 ccaggcgatc gtcgcccaga gaggcaatat ccgcgttaat gccgcaatga ccagcaaagg
    62461 ccatttccgc cagcgttacc agcaggccgc catcagagcg gtcgtggtat gcgagcagct
    62521 tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc gcgacgttgc
    62581 gtacatctgc cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca
    62641 gcgcgttatt gcctttgccc aaatcaatca gcagcagtgc gttatcttcg gtagaaagct
    62701 gcggcgtgat ggtgtgacgt acatcttcca cgcgggcaaa tgcagaaatc accagcgaca
    62761 gcggcgacgt catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag
    62821 agtctttacc caccgggatc gtcaggccca gcgccggaca aagctcttcg cccacggctt
    62881 taacggcttc atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg
    62941 cggaaagttt aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt
    63001 caccgaccgc cagacgggca gaggcggcga aatccagcag cgcaaccggc gcacgctcgc
    63061 caatcgccat cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag
    63121 cgaccggcac ctgccacgga cccaccatct gatcgcgcgc caccatgccg gttacgctgc
    63181 ggtcaccgat ggtcaccagg aaagttttct ccgccacggt cggcagatgc agtacgcgtt
    63241 tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc atcgcccttc gctttcagcg
    63301 tttgtacatc gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga
    63361 tcggctgatt atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac
    63421 caatcaccgc gtagggcgcg cgttcacgct tacacagttc gtcaaacagc ggtaactgat
    63481 cggcagcaac cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc
    63541 tcatgcccgg ttcgtcgttc agaatatcgc gcagttcgaa tttaccgccg cgtccgccgt
    63601 cgctcaccag ttccggcatc gcgttagaaa gaccgcctgc gccgacgtcg tggataaaca
    63661 gaattgggtt agcatcacca agctgccagc aacggtcgat cacttcctgg cagcgacgct
    63721 ccatctccgg gttgtcgcgc tgaacagaag caaagtcgag gtcggcatca gactgaccag
    63781 acgccataga agacgccgcg ccgccgccaa gaccgatgtt catcgccggg ccgccgagaa
    63841 cgaccagttt cgcaccaacg ttgatctcgc ctttttgtac gtgatcggca cgaatgttgc
    63901 cgatcccgcc cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccgttgtggc
    63961 tgttcacttt ttcttcataa gtacggaagt agccgttcag tgccggacga ccaaattcgt
    64021 tgttaaacgc cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg gtgacaatgc
    64081 gctcaggctt accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg
    64141 aaacggagaa accaaccaaa cccgctttcg gcttcgcgcc gcgcccggtg gcaccttcat
    64201 cgcggatttc accgccggaa ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt
    64261 ggttgtgagt ttcgactttc atcagaatat gcgccggttc ctgatggaaa tcgtagcggc
    64321 ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat
    64381 ctttataagc agagagaacg tgatctggcg tggtttcgaa agtatttttg atcatcctga
    64441 acagcgattt cggctgttgt tcaccgtcga taatccagtc ggcgttaaaa attttgtggc
    64501 ggcagtgctc agagttcgcc tgagcgaaca tatacagctc gatgtcgttc gggttacgac
    64561 caagctttgt gaaagcatcc tgcagatagt caatttcatc ttccgccaga gccaggccaa
    64621 gacgcaggtt agcgtcgatc agcgcctgac ggccctgccc cagcaaatca acgcttgtga
    64681 ccggagtcgg ttgatggtgg gcaaacaact gctctgcatc atctaaatca aaaaagaccg
    64741 tttccatcat gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg
    64801 taccggcttc tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc
    64861 agttatgggc aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg
    64921 tcaccagcag gagtttgcct tgcggggcgt ggctggcgag tgccggacca tatttcagca
    64981 ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga
    65041 catactcggc gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt
    65101 tgttgattcg gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct
    65161 cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg gcgtcattat aaagaatctg
    65221 atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg
    65281 ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt
    65341 atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga
    65401 attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg
    65461 ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc
    65521 gccattcaag cgcggggaga gttgcgtgtg agcaccattc atactcccct gacttataac
    65581 gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt tgccgattac
    65641 ctcggtgtaa aactgaaagt gaccgtgcgg cagaatatca gtcagctgtt tgacgacctc
    65701 gataatggta acgccgacct gctggcggca ggactggtct ataacagtga gcgggtaaaa
    65761 aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta taaagtgggt
    65821 cagtatcgcc cgcgcacact gggcaacctg acggcggagc aactcaccgt tgcaccgggt
    65881 cacgtggtgg ttaacgatct ccaaaccctg aaagacacaa aattcccgga attaagctgg
    65941 aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga aggaaaactc
    66001 gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggagctc
    66061 gccgtagcgc tcgatatcac cgatgaacaa ccggtgacct ggtttagccc gttagatggc
    66121 gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtacg
    66181 ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg
    66241 cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa
    66301 tacgccgaag aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg
    66361 gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaatacc
    66421 gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag cggtggcgtg
    66481 cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgagcggatc
    66541 tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc ccgcgccctg
    66601 acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg tctgccttta
    66661 cttagccaga aaccctatta cagcaagctg acttacggct acgctcgtgg acatgaagcc
    66721 tacgcttatg tcgaaaatat tcgtaagtat cagattagcc tggtgggtta tctgcaagag
    66781 aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg
    66841 cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca
    66901 aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga agagaaacaa
    66961 aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga
    67021 aagaagtcac tgagcaacgc tgcgcactca tccgccagta ttccttccgt aatttccact
    67081 cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc gccagttttc
    67141 gcgtcatgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac
    67201 attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc
    67261 accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc atggcgacca
    67321 atcgggcggt tccagccttc gccgattacc cgattgttat gcactaatac cgcgccgacc
    67381 ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg catccagtat
    67441 tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt
    67501 atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttact
    67561 cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc gctatagcca
    67621 ctgtacagcc gcagcggagt gccgattttg cgctccagtt gtgcgacctt ttcatgtccc
    67681 agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct ggcgataaga
    67741 ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag
    67801 ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga
    67861 accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc
    67921 tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact catcggccag
    67981 cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa cggcaggaca
    68041 agtaacgcat tcagcggttg gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc
    68101 tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa
    68161 ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag cctaacagat
    68221 agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca
    68281 tggcaattag cggcaatgga atataaaatt cactcgcgtg tctctcatat tgcctgttgt
    68341 cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac
    68401 caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg
    68461 gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc
    68521 gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt ggcgttgagt
    68581 gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca aatccgcggt
    68641 gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac
    68701 gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct
    68761 gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc
    68821 tggaagctga tgaagattgg cttcaatgct gccgcagtgc gcgatatgca tgcgctactc
    68881 gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta
    68941 cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggcg
    69001 attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt
    69061 gccgaagaac aagcgacaaa cagcgcttca atctctgctt gtcacgctca gggaatgtta
    69121 acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt
    69181 cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc
    69241 gcccggtttg tgttgttttg agagtttcct tatggcgttg ttaatcacta aaaaatgcat
    69301 caattgtgat atgtgtgaaa tcatataaaa acacatttat tttcatagtg atacagtaat
    69361 gtgttattga atgtgttgtt ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac
    69421 acaaagcctc cccctttctt tagcattttc tactgagcag ctatccgctt tcagacctca
    69481 cttaaattac gcttattcag agaagagcgt cagaaacacg atcggcatca ctgtttctgc
    69541 aaacatccct gtggcagtct ttatttgcca tataacatag cactaaagat cttcattaat
    69601 gtttccagta tagttaatac ttcattgtgt cgggggtgtg gtcatgcccc cgttttttat
    69661 tactcctgtt cccttaattg cactgccagg tgccacaaca cacatcaact gtggctggta
    69721 tggaaacacc aaaactctca cttccggaaa tctgaaaaat catccatatc aacaggatgc
    69781 tctggaaatg agaccggtat cactttcagg attaactttt ccgtggcact ctgcatccgt
    69841 gtaggtaaag tctcttcaca acaattagat aatcgactca gttcatgggg gaggtgatgg
    69901 ttcccccatc tttttgtctg ttactcctgt gcccagccaa ccctgtatgc caggatctca
    69961 tccgcactgg tcagcttttc cagctccttc ttcatggtgc gctgccggac gtggatttcc
    70021 atccctttgg tgaacatcgc ctgctctgcc gcttcactca gcgctataag ctcttctgct
    70081 gtcacttcca catcattgtt ttccgcatcc gtccagaaaa acgcctccgg cagtttcccc
    70141 gctttcgctg ccgccaccga tggctcaaga cgcgtctgcg ttgacttccc gtagtcccat
    70201 ttacgcccat tgtgctcaaa cgtgtagttc gccgcttcca tcgcattacg ccaggcgtta
    70261 atttcatcgc ccttcatccc tcgcgctttc tccgcggtca gcaggtccgt gattttctcc
    70321 ccgtcaaaac cccagcgccc cgagatgtca gctttgcggt tttcttccgt atccgccacc
    70381 tccaccacac tctggttcac aggccacaga actgatgcca ccctgccgat gccggtaatg
    70441 accccggcac tgttataaac caccttcagc gtctctgctg aaaataacga ctgacactca
    70501 taccagtcct gaccatcctc tgatttcagg tacatcgcac ccggcacatc cggattttcc
    70561 ggcgtgtatt gtctgaaatt ctttatgtgc atctgtaagt cctcccggtt acatgtctga
    70621 aagatgatgc gctttataat ggaaaatact caaaccccga agctcctgac gacacatact
    70681 cacccacgct gactgtacgc cactgagaac catcaaatgt cacctgaagc ggcctgtaag
    70741 ataccctgtc aataagggtt ttgtttccgt cgttgtaacc gccaatcccc gtaatcattg
    70801 caccaatatc caaatccact gcccatgtag tgctgtctgt ttgcggcgca taagtctgtt
    70861 tattacccag cctgataccc acaacccggc tcttcccgtc tgatgccccg ggcggtccca
    70921 tcggcccggt ttctccacgc tcacctctgg gaccggggtc gcctttctcc cctttcggtc
    70981 cctgcgctcc tcgcggacca gcggggcctg ttgctcccgt ttcacctttc ggaccctgtg
    71041 ctccggggtc acctttctct cctttcggac cgggaacacc acctcctgct gcagcctctt
    71101 ctgcctttgt tttcgcttca tttgccacat ccattgccgc cttcacggcc ttcggcgtag
    71161 ctgcctttgt ttcgtcatta ctgccagtat cactgcttaa ctgcaccaca cctttctgcg
    71221 tcgtgctggc atcccgctgg ctgaccgtat ccggtttgtt ctttgtgctg taatccaccc
    71281 gccagtggaa atactgtgtg tcgccgtaaa ccgtacaggt acagaccgtg ccattaataa
    71341 accccgttgc accgtattcg ccaatggtga cccggactat ggctgcctca tacttcccca
    71401 tcacctcaat gacacagcca cccagattaa gttttcccgg tccgacatcc gtgatgacct
    71461 tattgaatcc cgcatgcagt gatgccttca tcatccagta aggctggtca aatgcccctt
    71521 tttctttcag ccaggcgaca aactcgctcg tggtccattc accggccccc gtatgaatgt
    71581 cccgcccgta tactctggct gctcccacgg tgttcagaaa cttcacctta tccgggatat
    71641 cgtcaccgtt tttcgctttc tccaggcatc cgtccgcttt gtccattgcc gctttcaccg
    71701 ctttcggggt ggctgccttc gtttcatcat cactgtccgt tgcgctgctt aactgcacaa
    71761 ttcccttctg tgccgtcgtc gcatccggtc cgcccggctc gcctttttcg cccttctctc
    71821 cccgttcacc tttcacaccc tgaagcccct gcggccccct ctcaccacgc tcaccctttg
    71881 gcccacgctc accggtatcg ccttttggcc cgggaatacc ctgtggcccg gtgtctccct
    71941 tatccccctt cggtccccgc gcattctctg cccgtttctt cgcttcctct gcactcgccg
    72001 ctgacgcttc cgcacgtttc aggatttccg ctgccaccgc ttccagctct gcaagggctt
    72061 tcgggtaata ctgtgcgtct tccaggtcca tcagaaattt attcagcgtt cccggtgcag
    72121 aatccgcctt caccagaatg tcacccacat atgacggcgc gtacccttcc gtgttcagcg
    72181 tcacccggta cagacccggc tcaacatcca tactgtaact gccggtttcc cccggctgac
    72241 catacgccac cgtggtgaca atcaccgtct ccgttgtgcg tcgcgctttc agctctatcg
    72301 tgcatcccgg taccggtttt cccgtaccat ccttcagcac acccgatatt ctgactgtca
    72361 tggatttccc ccataaaaaa accgcagtac cggtttcccg gcctgcggta atatttgtgg
    72421 tttgttggtg ttaaaacggt gccatccggc tgaccaccct cagcaaccgg tcggcggggg
    72481 atattctccc ccgccacggt ttcttactgc ttacactgta agaacgccgc aatctccgcg
    72541 cccgctatcc ggaaccggaa ctcgcacagt gaactgtggg tgatccagat aatgagcact
    72601 accgtgatac aaatcacggt ggtttttaac ggtttttgcg acataaacgc ttgctccttt
    72661 tacggagagg cgctaacctt tcacttgctt aggatgaatg gtcaggcctc gggttaaaca
    72721 tgaatgtttg tccggggcct tttcacatcc ggccttcagg tgttccctcc ggccatcagc
    72781 caaaggcacc cgcgcatact gtacggtttt tgtctc
//
LOCUS       DAZFRN010000003        66029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFRN010000003 DAZFRN010000000
VERSION     DAZFRN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.003"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(182..1129)
                     /gene="dusC"
                     /locus_tag="ACHKUB_000153"
     CDS             complement(182..1129)
                     /gene="dusC"
                     /locus_tag="ACHKUB_000153"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO6653238.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1368..1766
                     /gene="yohJ"
                     /locus_tag="ACHKUB_000154"
     CDS             1368..1766
                     /gene="yohJ"
                     /locus_tag="ACHKUB_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO6653239.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1763..2458
                     /gene="yohK"
                     /locus_tag="ACHKUB_000155"
     CDS             1763..2458
                     /gene="yohK"
                     /locus_tag="ACHKUB_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO6653240.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2588..3472
                     /gene="cdd"
                     /locus_tag="ACHKUB_000156"
     CDS             2588..3472
                     /gene="cdd"
                     /locus_tag="ACHKUB_000156"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO6653241.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3622..4341
                     /gene="sanA"
                     /locus_tag="ACHKUB_000157"
     CDS             3622..4341
                     /gene="sanA"
                     /locus_tag="ACHKUB_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO6653242.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4344..4583
                     /gene="yeiS"
                     /locus_tag="ACHKUB_000158"
     CDS             4344..4583
                     /gene="yeiS"
                     /locus_tag="ACHKUB_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO6653243.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4902..6140
                     /gene="preT"
                     /locus_tag="ACHKUB_000159"
     CDS             4902..6140
                     /gene="preT"
                     /locus_tag="ACHKUB_000159"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO6653244.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            6134..7369
                     /gene="preA"
                     /locus_tag="ACHKUB_000160"
     CDS             6134..7369
                     /gene="preA"
                     /locus_tag="ACHKUB_000160"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO6653245.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKDEKEHPVTL"
     gene            complement(7440..8450)
                     /gene="mglC"
                     /locus_tag="ACHKUB_000161"
     CDS             complement(7440..8450)
                     /gene="mglC"
                     /locus_tag="ACHKUB_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO6653246.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8466..9986)
                     /gene="mglA"
                     /locus_tag="ACHKUB_000162"
     CDS             complement(8466..9986)
                     /gene="mglA"
                     /locus_tag="ACHKUB_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO6653247.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(10047..11045)
                     /gene="mglB"
                     /locus_tag="ACHKUB_000163"
     CDS             complement(10047..11045)
                     /gene="mglB"
                     /locus_tag="ACHKUB_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO6653248.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11325..12365)
                     /gene="galS"
                     /locus_tag="ACHKUB_000164"
     CDS             complement(11325..12365)
                     /gene="galS"
                     /locus_tag="ACHKUB_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO6653249.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(12507..13664)
                     /gene="yeiB"
                     /locus_tag="ACHKUB_000165"
     CDS             complement(12507..13664)
                     /gene="yeiB"
                     /locus_tag="ACHKUB_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO6653250.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13681..14349)
                     /gene="folE"
                     /locus_tag="ACHKUB_000166"
     CDS             complement(13681..14349)
                     /gene="folE"
                     /locus_tag="ACHKUB_000166"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO6653251.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14607..15443
                     /gene="yeiG"
                     /locus_tag="ACHKUB_000167"
     CDS             14607..15443
                     /gene="yeiG"
                     /locus_tag="ACHKUB_000167"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6653252.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15475..17466)
                     /gene="cirA"
                     /locus_tag="ACHKUB_000168"
     CDS             complement(15475..17466)
                     /gene="cirA"
                     /locus_tag="ACHKUB_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO6653253.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17759..19228)
                     /gene="lysP"
                     /locus_tag="ACHKUB_000169"
     CDS             complement(17759..19228)
                     /gene="lysP"
                     /locus_tag="ACHKUB_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO6653254.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19433..20314)
                     /gene="yieE"
                     /locus_tag="ACHKUB_000170"
     CDS             complement(19433..20314)
                     /gene="yieE"
                     /locus_tag="ACHKUB_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO6653255.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20413..21462
                     /gene="yeiH"
                     /locus_tag="ACHKUB_000171"
     CDS             20413..21462
                     /gene="yeiH"
                     /locus_tag="ACHKUB_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO6653256.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21536..22393
                     /gene="nfo"
                     /locus_tag="ACHKUB_000172"
     CDS             21536..22393
                     /gene="nfo"
                     /locus_tag="ACHKUB_000172"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO6653257.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22397..23485
                     /locus_tag="ACHKUB_000173"
     CDS             22397..23485
                     /locus_tag="ACHKUB_000173"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6653258.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23541..24791)
                     /gene="nupX"
                     /locus_tag="ACHKUB_000174"
     CDS             complement(23541..24791)
                     /gene="nupX"
                     /locus_tag="ACHKUB_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6653259.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24891..25832)
                     /gene="rihB"
                     /locus_tag="ACHKUB_000175"
     CDS             complement(24891..25832)
                     /gene="rihB"
                     /locus_tag="ACHKUB_000175"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO6653260.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25962..26660
                     /gene="yeiL"
                     /locus_tag="ACHKUB_000176"
     CDS             25962..26660
                     /gene="yeiL"
                     /locus_tag="ACHKUB_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO6653261.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26731..27981)
                     /locus_tag="ACHKUB_000177"
     CDS             complement(26731..27981)
                     /locus_tag="ACHKUB_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6653262.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(28075..29013)
                     /gene="psuG"
                     /locus_tag="ACHKUB_000178"
     CDS             complement(28075..29013)
                     /gene="psuG"
                     /locus_tag="ACHKUB_000178"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO6653263.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(29001..29958)
                     /gene="psuK"
                     /locus_tag="ACHKUB_000179"
                     /pseudo
     CDS             complement(29001..29958)
                     /gene="psuK"
                     /locus_tag="ACHKUB_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
     gene            complement(30382..32073)
                     /gene="fruA"
                     /locus_tag="ACHKUB_000180"
     CDS             complement(30382..32073)
                     /gene="fruA"
                     /locus_tag="ACHKUB_000180"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO6653264.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(32090..33028)
                     /gene="fruK"
                     /locus_tag="ACHKUB_000181"
     CDS             complement(32090..33028)
                     /gene="fruK"
                     /locus_tag="ACHKUB_000181"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO6653265.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(33028..34158)
                     /gene="fruB"
                     /locus_tag="ACHKUB_000182"
     CDS             complement(33028..34158)
                     /gene="fruB"
                     /locus_tag="ACHKUB_000182"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO6653266.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34526..35707
                     /gene="setB"
                     /locus_tag="ACHKUB_000183"
     CDS             34526..35707
                     /gene="setB"
                     /locus_tag="ACHKUB_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO6653267.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35704..35958)
                     /gene="yeiW"
                     /locus_tag="ACHKUB_000184"
     CDS             complement(35704..35958)
                     /gene="yeiW"
                     /locus_tag="ACHKUB_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6653268.1"
                     /translation="MKCRLGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36113..36685
                     /gene="yeiP"
                     /locus_tag="ACHKUB_000185"
     CDS             36113..36685
                     /gene="yeiP"
                     /locus_tag="ACHKUB_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO6653269.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36908..38374
                     /gene="yeiQ"
                     /locus_tag="ACHKUB_000186"
     CDS             36908..38374
                     /gene="yeiQ"
                     /locus_tag="ACHKUB_000186"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6653270.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38492..39478
                     /gene="yeiR"
                     /locus_tag="ACHKUB_000187"
     CDS             38492..39478
                     /gene="yeiR"
                     /locus_tag="ACHKUB_000187"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO6653271.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39517..40230
                     /gene="lpxT"
                     /locus_tag="ACHKUB_000188"
     CDS             39517..40230
                     /gene="lpxT"
                     /locus_tag="ACHKUB_000188"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO6653272.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40642..41208
                     /gene="mepS"
                     /locus_tag="ACHKUB_000189"
     CDS             40642..41208
                     /gene="mepS"
                     /locus_tag="ACHKUB_000189"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO6653273.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41389..42945
                     /gene="pdeN"
                     /locus_tag="ACHKUB_000190"
     CDS             41389..42945
                     /gene="pdeN"
                     /locus_tag="ACHKUB_000190"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6653274.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFTALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            43027..44841
                     /gene="yejA"
                     /locus_tag="ACHKUB_000191"
     CDS             43027..44841
                     /gene="yejA"
                     /locus_tag="ACHKUB_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO6653275.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPTVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44842..45936
                     /gene="yejB"
                     /locus_tag="ACHKUB_000192"
     CDS             44842..45936
                     /gene="yejB"
                     /locus_tag="ACHKUB_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO6653276.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45936..46961
                     /gene="yejE"
                     /locus_tag="ACHKUB_000193"
     CDS             45936..46961
                     /gene="yejE"
                     /locus_tag="ACHKUB_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6653277.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46963..48552
                     /gene="yejF"
                     /locus_tag="ACHKUB_000194"
     CDS             46963..48552
                     /gene="yejF"
                     /locus_tag="ACHKUB_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO6653278.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(48556..48900)
                     /gene="yejG"
                     /locus_tag="ACHKUB_000195"
     CDS             complement(48556..48900)
                     /gene="yejG"
                     /locus_tag="ACHKUB_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO6653279.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49233..50423)
                     /gene="bcr"
                     /locus_tag="ACHKUB_000196"
     CDS             complement(49233..50423)
                     /gene="bcr"
                     /locus_tag="ACHKUB_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO6653280.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(50451..51146)
                     /gene="rsuA"
                     /locus_tag="ACHKUB_000197"
     CDS             complement(50451..51146)
                     /gene="rsuA"
                     /locus_tag="ACHKUB_000197"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO6653281.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51295..53055
                     /gene="radD"
                     /locus_tag="ACHKUB_000198"
     CDS             51295..53055
                     /gene="radD"
                     /locus_tag="ACHKUB_000198"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO6653282.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53180..53464
                     /gene="rplY"
                     /locus_tag="ACHKUB_000199"
     CDS             53180..53464
                     /gene="rplY"
                     /locus_tag="ACHKUB_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO6653283.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53602..54609)
                     /gene="yejK"
                     /locus_tag="ACHKUB_000200"
     CDS             complement(53602..54609)
                     /gene="yejK"
                     /locus_tag="ACHKUB_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO6653284.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54791..55018
                     /gene="yejL"
                     /locus_tag="ACHKUB_000201"
     CDS             54791..55018
                     /gene="yejL"
                     /locus_tag="ACHKUB_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO6653285.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            55038..56798
                     /gene="yejM"
                     /locus_tag="ACHKUB_000202"
     CDS             55038..56798
                     /gene="yejM"
                     /locus_tag="ACHKUB_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO6653286.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56873..56949
                     /locus_tag="ACHKUB_000203"
     tRNA            56873..56949
                     /locus_tag="ACHKUB_000203"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56907..56909,aa:Pro,seq:ggg)
     gene            57168..58409
                     /locus_tag="ACHKUB_000204"
     CDS             57168..58409
                     /locus_tag="ACHKUB_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO6653287.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            58508..58738
                     /locus_tag="ACHKUB_000205"
     CDS             58508..58738
                     /locus_tag="ACHKUB_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653288.1"
                     /translation="MQKNFRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNMAESIDRVVRPATMKTGGRPKKWR"
     gene            58906..59112
                     /locus_tag="ACHKUB_000206"
     CDS             58906..59112
                     /locus_tag="ACHKUB_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6653289.1"
                     /translation="MEAIKKVIFRQEVKKLIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEEDVIAWMKEREQEARGTAA"
     gene            complement(59257..59436)
                     /locus_tag="ACHKUB_000207"
     CDS             complement(59257..59436)
                     /locus_tag="ACHKUB_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653290.1"
                     /translation="MSIHKNERRGNPPFQFRLDPDLREKMEKAQQHDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            complement(59656..59883)
                     /locus_tag="ACHKUB_000208"
     CDS             complement(59656..59883)
                     /locus_tag="ACHKUB_000208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653291.1"
                     /translation="MTNNTLSPIQDTQTQDDEIIRQRQSEACARLEEELTRTKIPPPA
                     PRLVPPEKFALEDFVDKYPRRLKSGKNRPPG"
     gene            complement(59941..60120)
                     /locus_tag="ACHKUB_000209"
     CDS             complement(59941..60120)
                     /locus_tag="ACHKUB_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6653292.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            60317..60517
                     /locus_tag="ACHKUB_000210"
     CDS             60317..60517
                     /locus_tag="ACHKUB_000210"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6653293.1"
                     /translation="MRNHITHPQGRDPHNLNKYIWRFIALSTAKPRVIHIVATSEQEA
                     RRQSPAGCVMVFTARIRQGVDL"
     gene            60514..60747
                     /locus_tag="ACHKUB_000211"
     CDS             60514..60747
                     /locus_tag="ACHKUB_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653294.1"
                     /translation="MSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLSDEQERRTLISNEKKCGGVHHVQ"
     gene            60737..60997
                     /locus_tag="ACHKUB_000212"
     CDS             60737..60997
                     /locus_tag="ACHKUB_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653295.1"
                     /translation="MCNNIRPDATAAALTTLMLALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGLCVMIVLIP"
     gene            60970..61203
                     /locus_tag="ACHKUB_000213"
     CDS             60970..61203
                     /locus_tag="ACHKUB_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653296.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            61196..61429
                     /locus_tag="ACHKUB_000214"
     CDS             61196..61429
                     /locus_tag="ACHKUB_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653297.1"
                     /translation="MRDIYHETTDRVFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            61435..61734
                     /locus_tag="ACHKUB_000215"
     CDS             61435..61734
                     /locus_tag="ACHKUB_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6653298.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            61731..63140
                     /locus_tag="ACHKUB_000216"
     CDS             61731..63140
                     /locus_tag="ACHKUB_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO6653299.1"
                     /translation="MKLAPNVKRLPKDKYTDAIIFAGIDAHSFAEHYIIAQAKKAGDP
                     VPPVYLGRYQLSELDNLQIVDDGRYRATVIRAGNIEEPQLLTIATKLASAGVQEARLL
                     SENFELLEEWSDQLPRLREAWERGESLVMKKIPQRKTKLPMSVGSTGYDTQLDYVVKG
                     IIPASSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPR
                     RVKAWEVVHDEQVKNLYLVNRPIFPAAPLDVEEMVIAARQVERETGKPVRMIILDTLA
                     RCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDA
                     EYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPR
                     PPVELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWL
                     YKLENDGVIVIDGDDVRPL"
     gene            63342..63587
                     /locus_tag="ACHKUB_000217"
     CDS             63342..63587
                     /locus_tag="ACHKUB_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653300.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            63718..63912
                     /locus_tag="ACHKUB_000218"
     CDS             63718..63912
                     /locus_tag="ACHKUB_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653301.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            63916..64077
                     /locus_tag="ACHKUB_000219"
     CDS             63916..64077
                     /locus_tag="ACHKUB_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO6653302.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            64205..64693
                     /locus_tag="ACHKUB_000220"
     CDS             64205..64693
                     /locus_tag="ACHKUB_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6653303.1"
                     /translation="MALTTKKKRFADALSRGANPTEATIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTPDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAASVYSNRFPIPKPPTDL
                     KH"
     gene            64705..64866
                     /locus_tag="ACHKUB_000221"
     CDS             64705..64866
                     /locus_tag="ACHKUB_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653304.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQSTEIRLQAAAVLMPYFHETLNPC
                     FQEEEEDDL"
     gene            64856..65779
                     /locus_tag="ACHKUB_000222"
     CDS             64856..65779
                     /locus_tag="ACHKUB_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6653305.1"
                     /translation="MICKNMKGVSAVRAASVLASCKFSHADAIEMTKRNDPAVSLFLE
                     KSVVAGMNTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLSTSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGAV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
BASE COUNT        16649 a        16936 c        16339 g        16105 t
ORIGIN      
        1 atcccttacg gctaccgggt cgggcgcgag gtaacatccc tgtaaaacgc gccctcagcc
       61 cacatccatg tgggctgccc cggccttcag ggaacgcctc ggcaattttg acgccaccaa
      121 acaaccgtgc ggcctattga taaagagcta acacattgtc aaaaaacatc actatggttt
      181 tttagagttt ctcgatatca attgcctgaa tagcccttgc aatatcaggg gaattattca
      241 acacccgaac atgctgaaat aatcccgttg cttcatcgta ttctttacgc aaataactca
      301 accactgttt aatccgcgca acgtgatata acccggtatc gccctgcttt tccagacggg
      361 tatatttttg cagcaaagca accacctccg gccacggcat tcgcggttcg ttatatttta
      421 ctacccggct caggttggga atattgagcg ccccgcgacc aatcatcact gcgtcgcagc
      481 cgctgatcgc aatgcattgt tgcgcgctct gccagtccca gatttcgccg ttggcaatca
      541 ccggaatatt cagccgctgg cgaatctcgc caatcgcctg ccagtcaata tgctccgcgc
      601 ggtaaccctg ctcttttgtc cgtccatgca ccaccagctc cgtagcacca gcctgttgaa
      661 ccgcatcggc gatttcaaat ttcttctcac cgctgtccca gcccagacgc actttcacgc
      721 tgacgggcaa atgcgccggt acagcttcac gcatcgcttt tgcaccctga tagatgagtt
      781 caggatcttt gagtaacgtc gccccaccac cgctaccgtt gaccgttttc gacgggcagc
      841 cgcaattgag atccacgccc caggaaccta attccaccgc acgggcggcg ttctctgcca
      901 gccattgtgg gaactgaccc aacaactgca cgcgcaccag cgtaccggat ggtgtccggc
      961 tggcgttttg tagctcaggg caaatgcgat gaaaaacttt taccggcagc agttgatcca
     1021 ccacgcggac aaactcggtg atgcacagat cgtagtcgtt aacttcggtc agcaattcac
     1081 gcaccagaga gtcaagcact ccctccatcg gtgccagtaa cacacgcata tcatcacccg
     1141 caaaataatg aggcgctatg ttagcgccta tggtcagcgg attaaaggtc tacaaagagg
     1201 gattgctgca aactgcaatc cctctctatg cttgatgtat gcctgagttc gttgcgcatt
     1261 gcgtaatgcc ggggagcgaa aaaataagcg ctgatttctt aatgtgatcg gtagcacgtt
     1321 ttacgaatta attgtatgat gaatccatct catctggggt gttgattatg agtaagacac
     1381 tgaacattat ctggcaatat ttacgcgctt tcgtcctgat ttatgcctgc ctgtatgcag
     1441 gcattttcat tgcttccctg ctaccggtaa ccattccggg cagcatcatc gggatgctga
     1501 tcctgtttgt cctgctggcc ttgcaaattc ttccggcgaa atgggtcaat ccggggtgct
     1561 acgtactgat tcgctatatg gcgctattgt ttgtgccgat tggcgtaggc gtcatgcaat
     1621 attttgattt gctccgcgcg cagtttggcc cggtagtggt ttcctgtgct gtcagtacgc
     1681 tggtggtttt tctggtggtg agctggagtt cacaactggt acacggtgaa cgtaaagtcg
     1741 taggtcagaa aggatcagaa gaatgatggc gaatatctgg tggtcattac cgctgacgtt
     1801 gattgtcttt tttgccgccc gcaaactggc ggcacggtat aagtttcccc tgcttaaccc
     1861 gttgctggta gcaatggtgg tgatcattcc ttttttaatg ctgaccggca tctcttacga
     1921 cagctacttt aaaggtagcg aagtgctaaa cgatctgctg caaccggcgg tcgtcgcgct
     1981 ggcctatcct ttatatgagc agctacacca gatccgcgcg cgctggaaat cgatcatcac
     2041 catctgtttt atcggcagcg tggttgcgat ggtgacgggg acttccgtgg cattgttgat
     2101 gggcgcttca ccggaaatcg ccgcgtcaat cctgccgaaa tcagtcacca cgcctattgc
     2161 aatggcggtt ggcggcagta ttggcggtat tccggcaatc agcgcagttt gcgtgatttt
     2221 cgtcggcatc ctcggcgcgg tatttggcca tacattgctt aatgcgatgc gtattcgtac
     2281 caaagctgcg cgcggtctgg caatggggac tgcctcgcac gccctcggta cggcgcgctg
     2341 cgccgagctg gattatcagg aaggtgcatt tagttcgcta gcgctggtgt tatgcgggat
     2401 aattacttcg ctgatcgcac cgttcctttt cccgattatt ctggcagtaa tgggctaaaa
     2461 tttgcgatgc gtcgcgcatt tttgatgtaa gtttcacgcg ttgcataatt aatgagattc
     2521 agatcacata taaagccata acgggttcgt aaactgttat cccattacat gattatgagg
     2581 caacgccatg catccacgtt ttcaaaccgc ttttgcccaa cttgcggata acttgcaatc
     2641 tgcactggaa cctattctgg cagacaagta cttccccgct ttgttgaccg gggagcaagt
     2701 ctcatcgctg aagagcgcaa cggggctgga cgaagacgcg ctggcattcg cactacttcc
     2761 gctggcggcg gcctgtgcgc gtacgccatt gtcgaatttt aatgttggcg caattgcgcg
     2821 cggtgtgagc ggaacctggt atttcggtgc caatatggaa tttattggtg cgacaatgca
     2881 gcaaaccgtt catgccgaac aaagcgcgat cagccacgcc tggttgagtg gtgaaaaagc
     2941 gcttgcagcc atcaccgtta actacacgcc ttgtggtcac tgccgtcagt ttatgaatga
     3001 actgaacagc ggtctggatc tgcgtattca tctgccgggc cgcgaggcac acgcgctgcg
     3061 tgactatctg ccggatgcct ttgggccgaa agatctggag attaaaacgc tgctgatgga
     3121 cgagcaggat cacggctatg cgctgactgg tgatgcgctt tctcaggcgg cgattgcagc
     3181 ggcaaaccgt tcgcacatgc cttacagtaa gtcgccaagc ggtgtcgcgc tggaatgtaa
     3241 agacggtcgt attttcagtg gcagctacgc tgaaaacgcc gcattcaacc cgactctgcc
     3301 accgttgcag ggagcgttaa ttctgttgaa tctcaagggt tatgattacc cggatatcca
     3361 gcgcgcggtt ctggcagaaa aagccgatgc gccgttgatt cagtgggatg ccacctccgc
     3421 aacgctgaaa gctctcggct gtcacagtat cgaccgagta cttctcgctt aagcctggtg
     3481 ccggatgcgg cgtgaacgcc ttgtccggct tgccagccct ctcctggtgt cgaaattccc
     3541 ggcaaacagt ttgccgtttc ttgcgcaaaa ccagcgggta aagtagcctg atggaaattt
     3601 tccttagatc gagtctcctg catgttaaag cgcgtgttcc tcagcctgtt agtcctgatc
     3661 ggcttgctgc tgttgactgt gctcggcctc gatcgctgga tgagctggaa aaccgcgcct
     3721 tatatctacg acgaattgca ggatctcccc taccgccagg tcggtgtggt gctcggaaca
     3781 gcaaaatatt atcgtactgg cgtaattaat cagtattatc gctaccgcat tcaaggagcg
     3841 attaatgcct ataacagcgg taaggtaaat tatctattac tgagcggcga taacgcattg
     3901 caaagttata atgagccgat gaccatgcgc aaagatttaa tcgctgctgg tgtcgaccca
     3961 tcagatattg ttctcgatta cgcaggcttt cgtacgctgg attccatcgt gcgtacacgc
     4021 aaagttttcg atactaatga tttcattatt atcacccaac gtttccactg tgagcgggca
     4081 ttatttattg cgctgcatat ggggattcag gctcagtgtt atgccgtacc gtcaccgaaa
     4141 gatatgctgt cagtacgtat tcgcgaattt gccgcccgtt tcggtgcgct ggctgacctt
     4201 tatattttta aacgtgaacc gcgtttttta gggccgctgg tccctattcc ggctatgcac
     4261 caggtgccgg aagatgcgca ggggtatccc gccgtcacac ccgaacagtt acttgaatta
     4321 caaaagaaac aaggaaagta gttatggatg tacagcagtt ttttgtcgtt gccgtttttt
     4381 tccttattcc gatattttgt ttccgcgaag catggaaagg ctggcgcgca ggcgcgattg
     4441 ataaacgggt taaaaatgca ccggaaccgg tgtatgtctg gcgagcaaaa aatcccggac
     4501 tctttttcgc ttatatggtg gcatatatcg gcttcggaat tttatctatc ggcatgattg
     4561 tttatcttat tttctatcgt taatattttc tcttttctgt taattattga gaataattat
     4621 tacttcactc aataagctgc ggatataaca ttctgaaccg cagctatttg tgaatccttt
     4681 cacagtataa atttccccac gcgcatagcc ttaatatcag cacgcaatat ttatactcta
     4741 aataattcga gttgcaggaa ggcgacaagt gagtgagtcc ccaggagctt acataagtaa
     4801 gtgactgggg tgagcgaacg cagacgcagc acatgcaact tgaagtatga cgagtatatt
     4861 tagcgttcgc cttaattact catagcatta aggaagatca catgccgcaa caaaattatc
     4921 tggatgaact cactccggct tttacgcctt tactggcgat taaagaagcc tctcgctgtt
     4981 tattatgtca tgacgctccc tgtagtcagg cttgcccggc acagaccgat ccggggaaat
     5041 ttattcgctc aatctacttt cgtaatttta aaggcgctgc cgagacaatt cgcgaaaata
     5101 atgccctcgg tgccgtttgt gccagagtgt gcccgacgga gaaattatgt caaagcggtt
     5161 gtacccgtgc cggtgttgat actcccattg atatcggtcg cttacagcgt tttgttactg
     5221 attttgaaca acaaaccggg atggaaattt atcagcccgg tactaaaacg ctcggcaaag
     5281 tcgcgattat tggcgcaggt cctgccggat tgcaggccag tgtaacactg acaaaccagg
     5341 gttatgacgt cacgatttat gagaaagaag cgcaccccgg tggctggttg cgtaacggta
     5401 ttccgcaatt ccggttaccg caatcagtgc tggatgcaga gatcgcccgt attgaaaaaa
     5461 tgggcgtgac cattaagtgc aacaacgaag tcggtaacac actcaccctt gagcagctga
     5521 aagcagaaaa ccgcgcggta ctggtcaccg tggggttatc aagcggttcc gggctaccgc
     5581 tgtttgagca tagtgacgtt gagattgccg tcgacttcct gcaacgtgta cgacaggcgc
     5641 aaggcgatat cagcattcca caaagcgcat taattatcgg cggcggtgat gtcgcgatgg
     5701 acgtagccag cacgctgaaa gttctcggct gtcaggcggt aacctgcgta gcgcgtgaag
     5761 agttagatga gtttccggca agcgaaaaag agtttgccag cgcccgggag ctgggcgttt
     5821 cgatcatcga tggattcacg ccagtagccg tggaaggcaa taaagtcacg tttaagcatg
     5881 tacggttacc gggcgaactg acgatggcgg cagataaaat tattctcgcc gtcggtcagc
     5941 atgccagact ggatgccttt gcggagttag agccgcagcg taacaccatc aaaacacaaa
     6001 attatcagac ccgcgatccg caagtctttg ctgctggcga tattgttgag ggtgacaaaa
     6061 ccgtggtcta tgccgtgaaa accgggaaag aagccgccga ggcgattcat cactatttag
     6121 agggagcttg ctcatgttaa cgaaagatct ttcgattact ttttgcggcg tgaagtttcc
     6181 caacccgttc tgcctctctt cttcgcctgt aggcaactgc tatgagatgt gtgccaaagc
     6241 ctacgacaca ggttggggcg gcgtggtgtt taaaacgatc ggctttttta tcgccaacga
     6301 agtctcgccg cgttttgatc atctggtgaa agaagatacc ggttttatcg gcttcaaaaa
     6361 tatggagcag attgctgaac atccgttgga agagaatctg gccgccctgc gtcagctgaa
     6421 ggaagattac ccggacaaag tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg
     6481 ggaggagctg gcgcgcctgg tgcaagaagc tggcgcggat atgatcgagt gtaacttctc
     6541 ctgtccgcaa atgacttctc atgcgatggg tagcgatgtc gggcaaagcc cggagctggt
     6601 agaaaaatat tgtcgggcag tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac
     6661 gccgaatatc ggtgatatgt gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg
     6721 cattgcggcg attaacaccg ttaaatccat caccaatatc gatcttaatc agaaaatcgg
     6781 tatgccgatc gttaacggaa aatcgagtat ttccggatat tccggtaaag cggtaaaacc
     6841 gatcgccctg cgcttcattc agcagatgcg cacccatcca gaactgcgcg atttcccaat
     6901 cagcggtatc ggcggcattg aaacctggga ggatgcggct gagtttttat tgctcggcgc
     6961 agcaacgtta caggtgacca ccggcatcat gcagtacggg tatcggatag tggaagatat
     7021 ggcaagcggg ttgtcgcatt atctcgccga tcagggattt gattcgctgc aggagatggt
     7081 aggtctggcg aataacaata ttgtcccggc ggaagattta gaccgcagtt atattgtcta
     7141 tccccgtatc aatcttgata aatgtgttgg ctgtggacgc tgttatattt cctgttacga
     7201 cggcggtcac caggcgatgg aatggagcga gaaaacccgc acaccgcatt gtaataccga
     7261 gaaatgtgtg ggttgtctgc tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg
     7321 ggaagtgaag tttaagaaag acgagaaaga acacccggta acgttgtaaa accagagacg
     7381 catccggcat ttggcaccga ttgccggatg cagcgcatcc ggcaatatcc acaatcacat
     7441 cacttcttac gcgcgtattt cagtgaatcc agcgctacgg cgaagataat aatcgcccct
     7501 ttgatgatgt actgccagta tgggtttacg ccgatatacg tcaggccata gttgatgacg
     7561 gtaaaaataa ttaccccggt caccacgcca atcaccgtcc ccacaccgcc gctgaacgat
     7621 acaccgccta ccacgcacgc cgcgatagca tccagctcat acataaagcc gaggttgttg
     7681 gtggcagagc cgatacgtcc ggcttctaac atcccgccaa aggcatagaa cacgccagac
     7741 aacgcgtaga tcatcagcag gttcaggccg acgttgacac cagatacttt tgccgcttcc
     7801 gggttaccgc caatggcaaa aatgttctta ccgaagcggg ttttgttcca caacacccag
     7861 acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag agagacggaa actccccagc
     7921 gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa aaccagaaat tggcgacgcc
     7981 ccgacaaagt cgtaatagag cgagttgatg ccatagacga tgatcatcgt gccgagcgtg
     8041 gtaatgaacg gcgtcacgtt gagataagcg ataatcagac cgttgatcaa accgatcacc
     8101 gcaccaatgg cacagacaat cagaataacc agcgcaatcg gcatcgtcgc catttccggg
     8161 aacactttgt tggcgttatc catggactgc aataatgtcg cagccaccac tgccgccagc
     8221 cctacctgac gaccagcaga aagatcggtc ccctgggtga caattaaccc tgccacaccg
     8281 agcgcgataa taatacgcac cgatgactgg gtgagaatat tacttaagtt caacagactt
     8341 aaaaatgttg ggtcctggaa aataataatc gccagcaaaa ctaaaagaac gacgtaaata
     8401 ccgccctctt tcaggtaagt aagaaaactt ttcttattta acgcactcat gggaagcccc
     8461 taatcttaaa ggtgcaaaga cgcaagacgc agaatttcgt tttgcgttgt tgtttttgta
     8521 tcgacaattc cggaaacgag accattgctc atgaccagaa tacggtctgt tatccctaac
     8581 aactcaggca tttcagagga gataataata atccccttgc ctttcttcgc cagttcggca
     8641 attaactgat aaatttcaaa cttcgccccg acatcaatac cgcgcgtcgg ttcatcgagc
     8701 attaatattt ctggttgcgt tagtagccag cgaccaataa tcaccttttg ctgattacca
     8761 ccggagagcg aaccaatttg cgtccgatga cccggcgttt ttacccgcat cgaatcaatc
     8821 acccactggg tatcgctttt catccgcgag ttatccagta aaccaacttt atttttgtag
     8881 ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca gataggcata aattcccgtt
     8941 gagcggcgct cctcagttac cagagcaaat ccgtggttta tggcttcatt ggcattatgg
     9001 ttattgatct ttttgccgtg caacgtaatg gtgccagccg atttctcgcg aataccaaat
     9061 aacgtctcaa caatatcggt acgtttcgcc cccaccagcc cggcaatacc gaggatctcc
     9121 cctttatgca gatcaaacga gacatcgcga atcgacggct ggcgcagtga cgtcaggtta
     9181 cgcacctcga ggatgacttc gcccggcttg ttttctttgt cagggaaacg ctgattaaga
     9241 gaacgcccaa ccatcatggc gatgatcttg tccatcgtca gtcctgccag cggttcggtg
     9301 gcgatccact gaccgtcgcg caatacggta acttcatcac ataactggaa gatttcttcc
     9361 attttatgcg agatataaac aataccgcag ccgcgctctt ttaatttacg aataatagtg
     9421 aacagatgat tgacctcttt ttcggttaac gaagaagtcg gttcatccat aatcacaatt
     9481 ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct gcatttgcga aacggataat
     9541 gtgccgacgc gcgcacgcgg atcgatatca atatccagtt catcaaaaat cgctttggtt
     9601 tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg tgggatatcg ccccagccac
     9661 atgttatcca tcaccgaacg ttgtaatacc aggtttaact cctggtgtac catcgaaata
     9721 ccattttcca gtgcctcttt ggcggaatgg aaatcgatct ctttaccctg gaataaaatg
     9781 gtgccggagt ctttttgata aataccaaac aggcatttta ataatgtcga ttttcctgca
     9841 ccgttttccc ccattaatgc atggatagaa tgtggccgga cttttaaatt aacgttatca
     9901 agtgccttaa caccaggaaa ggacttgttg ataccgctca tttccaacaa gtattccccg
     9961 gaagacggag tcgttgagct gaccatataa ttttaccttg ttggccatac aataagggcg
    10021 cagtaaacga ctgcgcccaa tcagtcttat ttcttgctga attcagccag gttgtcttta
    10081 tctacgccaa cataaggtac gcggaccact ttgttgtcga ttttccagtt ggtgccatca
    10141 gccgcacctt tgccatcggc caggtttttc gccagatcaa aggtcgcttt cgcctggttg
    10201 ttagcatcgt tcagtactgt gcccgccagt gcaccggatt tcaccagcgc cagcgcttct
    10261 ggcaacgcat cgacgccaaa caccggaatg ctggacttgt tgtgtgcttt cagtgcttct
    10321 accgcgccca ttgccatcgc atcgttgttg gcgataacca cttcgatttt gttggcgttc
    10381 gggccagaca gccaggcgtc catcttatct ttcgcctgag cggtatccca catagcggta
    10441 tctaactgta actgttcagt tttaatgcct ttgtcgttca gctctttaat cacgtaagtg
    10501 gtacgtgctt ctgcatccgg atggcccggt tcacctttca gcaatacgaa ttgaatctga
    10561 ccgtctttgt tcagatccca accctgattc gccgcccagt gtttagcaat caaatcgccc
    10621 tgaataatgc cggactcttt ggaatcagtg ccaacgtagt aggctttgtc gtagctatcc
    10681 agcgccttac gagacggttc tttgttgaag aaaaccaccg gcacgttttg cccacgcgct
    10741 ttctcaatca ccgtacccgc agctgccggg tcaaccaggt tgattgccag tgccttcacc
    10801 cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg actggtcatt ctgagaatca
    10861 ttcatcagca gctgaacatc tggcgcggct ttcgcatctt gctcaatagc cttgcgcact
    10921 acagacataa agttatcgtc gtacttatag attgttacac caatgcgagt atcagcagcg
    10981 tgtgcagcgg caccgaataa catgctggcc atcacagcag acagggttaa caccttctta
    11041 ttcatggtat ctccggtttt tcttatgcag agtagtgctt gagataaatg ctcggcgggg
    11101 cagtagagtt aatgaagtgt tactgaacgc cgaagctcac tttttaaaat tcgttcttcc
    11161 atgcccggta acgctccaga aaacggcttt aattgttgtt atgacgctat tacctcggtg
    11221 aaagtgatta ttcactgtta catacgggtt acaacgttaa aacggtgcaa tcatagctat
    11281 cacattgtta agatactgtg aaatcactca cagattgaaa gcggttacat cgcctgattt
    11341 gttgagttag tgatcgtcgc cgcattctgg cgtgttgcga cagaatggcg acgcactaac
    11401 gtcggcataa aacagtggct ggcacgagga tcaatattgc ctgctgcccc ctgcaaggcc
    11461 agttcggtgg ctaatttcgc cattgaagca atgggataac gcacggtcgt taattgcggg
    11521 tcggtgtaac gggcgatggg aatatcatcg aaaccgatga ttgagagatg taacggaatc
    11581 gcaatgccat tatcttttaa tgctgtcagt gcgccagcgg ccatattgtc gttataagca
    11641 aatacagcgg taagttgtag attgcgcccc agcagttcaa ccatcgccgc ctcaccgccc
    11701 ggcatgtccg gcgtaccagt gccaatccag ctttccggcg gaataatatc ctgctctttc
    11761 aacgcactca tccagcctgc tttacgcatg gcgtcatctt caatgccgtg gctggaagaa
    11821 agataaccaa tacgttgatg accgttattc agcaacatgc gcgtcgccat tcgggcaccg
    11881 ctgagattat ccaggcaaac gcaacgatgg gcgtaccccg gcacaacgcg gttgattaac
    11941 accataccgg gaatgttatc cataaattgc gccagttcat cgtcactcaa tgcttttgag
    12001 tgaacaatca acgcattaca acgctggcga attaacacct caatggcgtg acgctctttt
    12061 tccgcttcat gatagctatt gccgattagc acgtatttct gatgctgctg ggcgaccaga
    12121 tccaccgctt ttaccagcgc gccgaaaaaa gcatcagaaa cgtccatcac caccacgcca
    12181 atggtatcgc taacctgagt tgccagcgcc tgggcattgg catttggccg ataatccagc
    12241 tcactcacgg ctttcattac tgcttcacgc gtgtcggcac tgaccagcgt gctgttattg
    12301 agcacccggg aaaccgttgc cacagagacg ccagcctgac gcgctacatc acgaatggtg
    12361 atcatattca ctacctgtta tgagattgca gtaaatgact gcttgctggc ggctattttg
    12421 tcagcactga atacaggact tcgtgaatcg agtcacagca atggaaacgg ttacagccgt
    12481 tttgttaacg aatgtgatcc agatcgttat cttgatgttt tagatattgc cgttccggca
    12541 gcacgcaaag ttaactgacg ccagagccat tccaccggcc cctggcggaa gtaacgcagc
    12601 cagataacag agaagaggat attcgccagc cataccggaa taacaaacgc cagcagctcc
    12661 aggcgatcaa aatgcataaa caaaccgagg tggtaaaaaa gcgtggtaca aatcagcgtt
    12721 tgcaatagat agttggtcag cgccatccgt ccgacgcagg cgatcgcaag caccagctta
    12781 aagcggctta attgcggcca gaagccataa aacagcgacg catagccaat cgcctgaaac
    12841 ggcgcactca gttcccgcgg catttgaagt aagaaggcac accagcgata cgcccagtcc
    12901 agctgccatt gcagggcgat ggcaggaagg ttaatggtca ccccaatcgc caccagcaca
    12961 aaaccagtac gacgatagtg acgtaagctg aactgccctt tcagccagcc gctgcgcatc
    13021 agcgccgcac caatgagcat catcccagcc agttgccagc catactgtgc gcccagtgcc
    13081 agtaaactgt tgccaacacc atcggcacga ttactgatcg catcaacgcc gccgtgaagc
    13141 ttccagtatt tttcatacaa aatagccgat gcatccggcg tccaggcgcg gctggtctgg
    13201 ctgtcggaaa tcagccccaa taacagcaaa acgccaagcc ccaccagata aagcatgacg
    13261 ccggtattaa acaggctttt taccgatggc gcatcgcgca ccagccgcca gcagattaag
    13321 cccaccagcc cgtaagccag cagaatatcg ccgtcccaga acaataaacc gtgaataaag
    13381 cccaataaga ctaacagcgt taaccgcgac tggatccagc gtctgccacg gggcagcaac
    13441 atttgcagtc cagcaccaaa cagcagcgca aaaagcgtga ggaatttcac ctggccgatg
    13501 agatcaagaa atgcccaggt ccatgcatcc tgcggcgtaa tagcaccgta ccaggcggga
    13561 ttgagataag ccgcctttgg tagcccaaag gcgctgatgt ttaatagcag gatccccaga
    13621 atggcgacgc cgcgaacaaa atcgagcgtg acgttgcgct ccatggttcc tgccttttaa
    13681 tcagttgtga tgacgcacag cgcgcagaaa ctcgtggcgc gtattctgac tggatttgaa
    13741 caatccacca agagaggtcg ttgttgtggc actggttgca tcgcggatgc cacgcgcctt
    13801 cacgcagtaa tgcaccgcgt cgatcgagac agccacgtta ttggtgccca gcagcgtttg
    13861 tagcgcaata agaatttgct gcgtcagacg ttcctgcacc tgcggacgct gggcaaagaa
    13921 ctgcacgata cggttaattt ttgacagacc gatcaccgaa tctttcggga tataagccac
    13981 cgtcgctttg ccatcgatgg taacaaaatg gtgttcacag gtactggtca gagtgatatc
    14041 gcgcacggtg accatttcat cgaccttcat tttgttttca atgagggtga ttttcgggaa
    14101 gttggcgtaa tccagaccgg agaaaatttc atcgacatac attttagcga tgcgatgcgg
    14161 cgtttccatc aaactgtcat cagccaggtc gagattcagc agctgcatga tttcggtcat
    14221 atgaccagca ataaggcttt tgcgcgtttc gttatccatt tcatgcacgg gcgggcgcag
    14281 cggtgtttcc agtcctcgcg caactaacgc ttcatgaacc agggccgctt ctttactgag
    14341 tgatggcatt tatgatttct cctgcaggtg tgacgcctcc gccctgcgtg ggggcaaagt
    14401 tattaagctg atttacagcc tgattattgt gcgtgaggcg gcgcacataa tccagtattc
    14461 acagcgataa ttattgtaat tgccgctgcc tttcatcagc agatgttaaa acatcgttat
    14521 gcaaatacgg aagtgaaagt tactcacagc acattgaata aacggtatga tgaagaaatt
    14581 gcaaacaaca caacaaggag ccacgcatgg aaatgctcga agagcaccgc tgttttgaag
    14641 gctggcagca acgctggcga cacgactcca gtaccttaaa ctgcccgatg acgttcagta
    14701 tctttctccc accccctcgt gatcacaccc cgccaccagt gctgtactgg ctttccggat
    14761 taacctgcaa tgacgagaac ttcaccacca aggcgggtgc ccagcgcgtg gcggcagaac
    14821 tggggattgt actggtgatg ccagacacca gcccgcgcgg cgaaaaggtt gccaacgacg
    14881 atggctacga tttaggccag ggcgcaggct tttatcttaa tgccacgcaa ccgccgtggg
    14941 cgacgcatta ccggatgtat gattatctgc gcgatgaatt accggcgctg gttcagtcgc
    15001 aatttaatgt cagcgaccgc tgcgccatta gcggtcactc aatgggtggt cacggtgcgc
    15061 tgattatggc gctgaaaaat ccgggtaaat acaccagcgt ttcggccttt gcgccaattg
    15121 tgaatccgtg cagcgtcccg tggggaatca aagcgtttag cagctattta ggtgaggaca
    15181 aaaatgcatg gctggaatgg gacagttgcg cgctgatgta tgccagtaac gcgcaggatg
    15241 cgatcccgac gcttatcgat cagggcgata atgatcagtt tcttgccgac cagttgcaac
    15301 ctgcggtact ggcagaagcc gcgcgccaga aagcgtggcc gatgacgctg cgtattcagc
    15361 cgggatatga tcacagttac tacttcatcg cctcttttat agaggatcac ctgcgcttcc
    15421 atgcgcagta tttactgaag tgaaagtccg cccggttcgc cgggcatctt ctcatcagaa
    15481 gcgataatcc actgccataa agtaacgacg tccgtcttcg ttatagctgt agtcgtcacg
    15541 actgagatcc ttgtcgccaa ggttcagcac gcctgcgcgc agtttgacgt ctttagtcac
    15601 ctgccaggcc gcgccggtat tccagatggt ataaccgccc ggtgttttag ccgtcgcgct
    15661 gtcggcgcgt ttctgcccgg tatagtgacc agaaacatag aatgaccagt cttccagcgc
    15721 cagcggtttc cagtccagcg taccgttagc agtatggaac ggcagatcgg atagcggttt
    15781 gttttcgccg ttgctgacat cacgaccatc gttgtaggtg tagttgatcg acagtttcca
    15841 ttcatcgttg aacggaattt tcagttcggt ttccacgccc tgaatacgag ctttgttaac
    15901 gttgtagtag ctaaataccg gtatgcgccg tccgttagcg cccgtctcaa aaccaacaaa
    15961 gttttggtag cccggtgcag cgttgacgtc agacgtacgg ctgatgctga tacgatcttt
    16021 cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc cttcttcacc
    16081 catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat ccgggctacc
    16141 cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa gttgcaacag
    16201 agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt cggtggcgtt
    16261 ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcgtgat cgtccatacg
    16321 cacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcca caaacagcgc
    16381 gtactggctg gcagacgttt tggagctggt tcccccggtc aggttcaccg catcgctaag
    16441 tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg tcagcggcaa
    16501 cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt tgccagggtt
    16561 tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat cccaacgccc
    16621 attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg agtcggaatc
    16681 acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat tcggtgtcca
    16741 ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg tttctccggt
    16801 atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc tgccgtaagc
    16861 tttcattccc agcacaccat caattaatgg tccactggta aagaactgac cgttataggt
    16921 atcaccgcga tcgcgatgtt cctgaatggt ggtatcgacg gtaacggtac ccgaccattt
    16981 ctgaccgatt tttttggtga tgatattcac tacaccgccg agcgcatcgg agccgtatag
    17041 cgacgacatc gggccacgaa ccacttcaat acgttcgatg gaatcgaccg ggatccagtt
    17101 cagatcgaaa tcattgtggc ggaagacggc attgcgggag ttaacgcgtt taccgtcgac
    17161 gagaatcagg gtgtagctgc tgtccagacc acgaatacta acgcccttac ggttatcccc
    17221 ttcgttcgtc agttgtacgc caggcacttc tttgaggaca tccttcagat tctgtaccgg
    17281 ttttcgctgc aggtcttcct gggtaatgac gctgatactg gcgggtgcat ctttgaggtt
    17341 ttgttcaacg gaagatgcag tgacaaccat cgtttcgcca tcatcatcga ccgctaacac
    17401 aggccatgca caagaaatag cggacaaaca cagcccgacc cgtacgaaag ggttcaacct
    17461 aaacattcca tatctccatg aggtaactac gaaaataaaa tgggttatcg ctcacatctt
    17521 cttcacgtcc ccttgcgtac ggcagcatcg cggtggactt atttgtatgc aggtgatcat
    17581 cccagaaagc cggaacagcc tctgatttgt cagccttttt tgattgcggc gtaacgataa
    17641 tgcaaacgat aacaattatc aatccaaatg ttaaatttta tatccgcatg atttgtcagg
    17701 gaaatggaat tgaaaaagcc ctctcggttg agagggctta gcaaggaagg gaggaaactt
    17761 atttcttatc gttctgcggg aacttcattt cgctgtaacg tacgaagtga gttcctttaa
    17821 tcagcttgta gccgaaccaa ataatcagga acagcgggat accaatatac gttgccgcta
    17881 cgccgcccca gtcaatggta tctttcagga acgcttcgta gttctggccc aaagtgataa
    17941 tcagacacag aatgaatgcg aagatcggcc ccagtgggaa gaaacctgaa cggtacggca
    18001 gatcgttaat gtcgtgtccc tgcaatacgt aaccgcgacg gaagcgatag tggctaatgg
    18061 caatccccag ccaggcgata aaacccgtca tcccggaggt gttcagcagc cacaggtata
    18121 ccgtctggtt gccaaacatg gaggtcagga agcacagacc ggcaatcacc gtcgtcgcat
    18181 acagcgcatt acgcggcacg ccaccacgcg acagtttagc gaaaatgcgc ggcgctttac
    18241 cgtcacacgc cagggtgtac agcatacgag tagacgcata cataccggag ttacccgctg
    18301 acagcaccgc cgtcagaata actgcgttca tcaccgccgc cgcagagagc agacccgcgt
    18361 gctggaacac cagggtgaac ggactaacgc tgatgtcttt aacatcgtta cgcagcaggc
    18421 tcggatcggt gtacggaata atcaggctga taatcaggat cgcgaacaca tagaacaaca
    18481 ggattcgcca gaacacctga cgtaccgcgc gtggaatgtt tttcgccgga tcttcggact
    18541 cgcctgcagc aataccgatc agctcggttc cctggaaaga gaagccgaca atcatagcta
    18601 cgccgatcat cgccgcaaaa ccaccagcaa acggcgcttc gccgattgtc cagttgctcc
    18661 agcccgcagg ctgcgcgcct ttgaagatac cgataatcat cagcacgcca acgatgataa
    18721 agacaataac tgtcgtgact ttgatcagtg agaaccagta ttccgcttca ccaaagccac
    18781 gaactgagat atagttcagc aggaagataa cgccgaggaa caacgcactc cagatccagc
    18841 ccggtgtatc cgggaaccac cagctcatga ccagctgagc tgcaaccagg tcaacggcga
    18901 tagtcaccgc ccagttgtac cagtagttcc agcccagcgc gaagccaaag ccttcttcaa
    18961 catagttctg accgtaagtg gcaaacgaac cggaaaccgg catatatgca gccagttcac
    19021 cgagactggt catcaggaag taaaccatca ggccaatcag catatacgag agcaatgccc
    19081 cgcccggacc tgcctgagaa atcgttgcgc cagaggcaac aaaaagacct gtaccgatgg
    19141 aaccgccaat ggcaatcatc gtcaggtgac gcgcctttaa ttcacggcgt aagcccggcg
    19201 cttctgtggt tttagtttcg gaaaccatac aaaaatgcta tccatcttaa aaatgaggcg
    19261 cgattgtagc agacgattgg caatccttcc ggcagaaata cacggttata agacaccttc
    19321 atgatcgccc agggattata agtaaagcag ccaatctctt ttctggcgaa tgcatgctga
    19381 gggcgctgcg tcacgaaatc gacacagcac cagcatgttc ttgtacagca acttaacgcg
    19441 gtacattcgc gggatcgcaa tagtccagaa agcgccgtag cgcgttggaa aggtgttttt
    19501 gccgatgatg tatacgccac aacgtacgca tcaggcgcgg cagagggacc gcaacttcac
    19561 ttaatgtgcc tgcctgcaat tgatcttcaa tcacccgtcg cgacaggcaa ctaattccca
    19621 acccatgacg caccgcatgt ttgattgcct cggagttacc taattccatc gccatctcaa
    19681 acttcggtaa atgtgacagc aatagataat cgacaatctc ccgcgtgccg gaaccgcgtt
    19741 cacgcaggat ccacggcgca gcggccagct gctctaaggt gaccggacca cgggccaacg
    19801 gcgaagtcgg cgcggcgaaa accaccagct cgtcttccag ccacggttca gaaataattt
    19861 cagtgctgtg gcacggtcct tcaataaaac caatatcaac gcggaaatcc agcaccgctt
    19921 gcatcacgtc ctggctgttc ccaacgctaa gttcaatcgg caactgcgga taatcatggc
    19981 gataacgggc gataactgca ggcagaatgt agttaccgat ggtactactg gcatagatac
    20041 gaatcgcgcc gttgtcttcg cgaaacagtt gttcgatttc aaccgcctgt tcaagcaatg
    20101 ccaacgcacg cggatagagc agccgcccgt gttcattaac aaccagtctt ttccccacgc
    20161 gatcaaacag ttgcacgcca agctgccctt ccaggtcggt caaggctgcg ctcactgctg
    20221 attgcgacaa cgccagcatc accgacgcct gggtggttga tccacttttc aatacttctg
    20281 caaaaacttc caactgccgg agggtgatgt gcatagtcgc ttaccactta taaagattaa
    20341 ttataaatat ataatcaatt ttatttttaa accagttagt cgtaacctta tgcccggtta
    20401 aaggagaggg ttatgacgaa tatcacctta cagaaacaac atcgtacact gtggcatttt
    20461 attccggggt tagccctgag tgcagttatc accggggtcg ccctgtgggg tggttccatt
    20521 cccgcggttg cgggtgccgg gtttagtgcc ctcaccctcg caatcttgtt ggggatggtt
    20581 ttaggcaaca ccatctatcc gcacatctgg aaaagctgtg acggtggtgt gctgtttgcc
    20641 aagcaatatt tattacgtct gggtatcatt ctttatggct tccgtctgac gttctcgcaa
    20701 attgccgatg tcggtatcag tgggatcatc attgacgtgc tgacgctgtc cagtaccttc
    20761 ctgctggctt gcttcctggg gcagaaagtg tttggtctgg ataagcacac cagctggttg
    20821 atcggtgccg gtagcagtat ctgtggtgct gccgcggtac tggcgactga gccggtagtg
    20881 aaagcggaag ccagtaaagt aaccgtggct gttgcaaccg ttgttatctt cgggaccgtc
    20941 gcgattttcc tctacccggc gatatatccg ctgatgtctc aatggtttag tccggaaact
    21001 ttcggtatct acattggttc tactgtgcac gaagtggcgc aggtggtggc ggcaggtcat
    21061 gccatcagcc cggatgcgga aaacgcagca gttatttcca aaatgctgcg cgtgatgatg
    21121 ctggctccgt tcctcatcct gctggcggcg cgtgttaaac agctgtctgg ggcgaacagc
    21181 ggcgagaaaa gcaaaatcac tattccgtgg tttgccatat tgttcatcgt cgttgccatc
    21241 tttaactcgt tccacctgtt accgcagagc gtggtgaaca tgctggtaac gctggatacc
    21301 ttcctgctgg caatggcgat ggcggcgctg ggtctgacca ctcacgtcag cgcgctgaaa
    21361 aaagccgggg cgaaacctct gctgatggca ctggtactgt ttgcctggct gattgttggt
    21421 ggtggtgcga ttaactatgt gattcaaagc gtcatcgcat aaaccactac atcttgctcc
    21481 tgttaacccg ctatcattac cgttttcctc cagcgggttt aacaggagtc ctcgcatgaa
    21541 atacattgga gcgcacgtta gtgctgctgg cggtctggca aatgccgcaa ttcgcgccgc
    21601 cgaaatcgac gcaaccgcgt ttgccttgtt caccaaaaac caacgtcagt ggcgtgccgc
    21661 accgctcacg acgcaaacca tcgatgaatt caaagcggcc tgtgaaaaat atcactacac
    21721 atcggcgcaa attcttcccc acgacagcta tctgattaac ctcggacatc cggtcactga
    21781 agctctggaa aaatcgcgcg atgcctttat agatgaaatg cagcgttgcg aacaactggg
    21841 gctttctttg ctcaacttcc accctggcag ccatctgatg cagatttcag aagaggattg
    21901 ccttgcgcgt attgccgaat ccatcaacat tgcgctggat aaaactcaag gtgtaacagc
    21961 ggtgattgaa aacaccgccg gtcagggcag taacttaggg tttaaattcg aacatctcgc
    22021 ggcgattatc gacggcgtgg aagataaatc ccgtgtcggc gtctgcattg atacctgcca
    22081 tgctttcgct gccgggtatg atttgcgtac tccagccgaa tgcgagaaaa cgttcgcgga
    22141 ttttgcccgt attgttggct ttaagtatct gcgcgggatg caccttaacg atgcgaaaag
    22201 cacctttggc agccgcgttg accgccatca tagcctcggt gaaggcaata tcggtcatga
    22261 tgcgttccgc tggatcatgc aggacgaccg tttcgacggc attccgctga tccttgaaac
    22321 catcaacccg gatatctggg cagaagagat cgcctggctg aaagcgcaac aaactgaaaa
    22381 agcggtggcc tgatcgatga acgacaggga aaagcagatt cttaaaattt tacggcgtaa
    22441 cccgctgatt caacagaatg aaatcgccga tattctgcaa atcagccgct cacgtgttgc
    22501 cgctcatatt atggatttga tgcgcaaggg tttgattaaa ggcaaaggat atatccttac
    22561 cgagcaggat tactgtgtgg ttgtgggtgc aatcaatatg gatattcgcg gaatggcgga
    22621 tatccgttac ccgcaagcag cctctcatcc aggttctgtt cactgttccg caggcggcgt
    22681 cggacgtaat atcgcgcata acctggctct gcttggtcgg gatgtgcatt taatatcagc
    22741 tattggcaac gatttttacg gcgagacatt actggaagaa acgcgccgcg cgggcgtgaa
    22801 cgtttcaaac tgcattcgtc tgcatggaca cagcactgca acttatctcg ctatcgctaa
    22861 taagcaggaa gaaactatcc tggcaatcaa tgacacacat attctgcaac agctaacgcc
    22921 tcaactgctc aatacttcac gggacttgat tcgccatgct ggtgtggtgc tggctgattg
    22981 taatctcaca cctgaagccc tggagtgggt ctttaccatt gctgatgaaa ttccgatgtt
    23041 tgtcgatacc gtttccgaat ttaaagcgaa caaagtaaaa aactggtaca gccggatcca
    23101 cactctgaaa ccaacacaaa atgaactgga aattttgtgg gggcaaccga ttaaagacga
    23161 caacgatcgc atcagagctg taaattcgct gcatcaacaa ggtgttaaac gcatttttgt
    23221 ttatctgaaa gatgaatctg tattttgcag cgataaggat ggagaacaat ttttgctgac
    23281 cgcgccagcg catacgacag tagacagttt tggtgctgac gatggtttta tggcgggcct
    23341 ggtatatagc tttcttgaag gatacagttt ccgcgacagc gcccgttttg cggtagcctg
    23401 cgcggcaatt tcacgcgcca gcggcagctt aaacaaccct accctgtctg ccgataacgc
    23461 gctttcatta gtgccaatgg tgtaacaatg ttgccggatt cgacgcttga ccgcatctta
    23521 tccggctcat aaatcgcaca ctacgctaaa ccaataaaga acccggcaat ggtggcactc
    23581 atcaggttag aaagtgtcgc cgccgccagc gcgcgtaaac caagctgggc gatttccggc
    23641 gcacggtgtg gcgcaaccgc agaaaacgcc cccaccacca ccccgataga accaaagtta
    23701 gcgaaaccgc acaacgcgaa ggaaataatc gccacggttt tagcatccag agtgccagcc
    23761 gtttgcagat agggtgagaa attgagataa gcgacaaatt catttattgc cagtttctgt
    23821 ccaatcaaac tcccggcaag attcgcatca gtccagtcaa cacccattac ccacgccagt
    23881 ggggccagca ggtaacctaa aatggactcc agcgaggcat gttcaaaacc aaaccagccg
    23941 ccaacgccgc cgataatacc gttaattaac gcaatgatgg caacaaatgc catcaccact
    24001 gttgccacac ccgcagcgat tttcagcccg gtcatcgccc ctgtcgcggc ggcttcaata
    24061 atgctttttg gcggtgtttc ggtgaaagag aggttattaa aagaaacctg cgaagattcc
    24121 gtagccgggc ttaacaggcg ggcaaacaag atcccgcctg ggatcgccat taacgatgcc
    24181 gccagcaaat attccacagg tacgcccagt gcggcgtaac caatcattgt cgaaccggca
    24241 attgaggcca tgccactaca aatcgctgta aataattcat tgcgattcag ccgatcgata
    24301 aagggtttga cgattgccgg aatttcgttt tgcccgagga aaatggtagt gaccgcgacg
    24361 aatgactcga tcttgctgat atttaatgct ttctggaaga taccgccgag aatgcgaatt
    24421 aagatcccca tcacaccgat gtaatagaga atactcacca gcgcggtgac gaagataatt
    24481 gccggtaata ctctgaaacc aaagataaat cctgcgccat caaacagcgt gtccattttc
    24541 ggcccgacca gcgaaccgaa gataaatgcg ctacccgcgt cgctgtacgc catcacttta
    24601 tgcacgccaa aagcgacttt ttcagcgacc caacgccctg gcggtaacca aagcataatg
    24661 ccgccaatca caacctgtaa cactaacgcc gcgccaacgg tacgcaggct gatcttcttc
    24721 ttgtttactg acagcaaaaa tgcaattgcc agcaatacca ccattcccag aacacttctc
    24781 atgacatcca tagtgattat ctcttcatgt catattaggc gacggctttc tgcccctcag
    24841 aaatagccag gcctgcatct tgcgataccg gcccggcttg ttatcaacac ttaatgggtt
    24901 ttgatgtagc cgcgcacgca ctcttcgact aagccccaga accagtcggt atcaatagtg
    24961 atgccgactt tggtattggc gggcttgcca agaacgccca gctcgtcgca gacggtgcgc
    25021 ccatagcaag ggccactgtt gacgtccact tcgacgtaca tctcctgggt tttaatgcca
    25081 tcagggttaa tcagataacc gatgcaggtg gcgtcgtgca ccgggccgcc agccaggccg
    25141 tagttttcga actgcgtttt gagagtgaag ttcatgatgt cgctgaacag ctctccggca
    25201 gggccgcctg ccctttccat ccgtgcaatc acgtccgggg tgcaaacggt ctggttggtg
    25261 agatcgaggc ccatcatcac taatggaacg ccagaggtga acaccacgcg ggctgcttcc
    25321 gggtcagcaa agatgttgaa ctcggcagat ggcgtgaagt tgcctgtacc gtaagcgccg
    25381 cccattagca caatttcacg gattttgggc aggatcgcgg gttgcatacg cattgccacc
    25441 gcgatatttg aaagcggacc aaccggcacc agagtgatat cgccatcgct tgccatcagg
    25501 gtatcaatga tgtatttcac cgcgtgggta ctttctgcct ggcgggtcag cggctcgaat
    25561 accgggccat ccagtccggt ttcaccgtga atattatcgg caacgatttg ttgacgcata
    25621 atgggttgcg gcatccccgc ataaaccgga acattaatct ccagtttctg gcaaacattc
    25681 aggccattaa ttaatgtttt atcaagcgtc tgattacccg ctacaatagt gatgcctaat
    25741 aaatctattg ccggatgttt cgctgccatc atcattgcaa tagcatcatc atgacccgga
    25801 tcacaatcca gaataatttt tctcttttcc attgtttatt tcctctgttt ccagttgcgt
    25861 tattttttct acagcaaaga aaattcgacc gggccgatga ttgaatctta ccaaccgcgt
    25921 acgtatgcta aatatgagaa atctcatagc ggataaacat cgtgaaagaa atccacaata
    25981 atgatcttaa gcagcaattg atgagtgaat ccgcgtttaa ggattgcttt ttaacggatg
    26041 tttcagccga tacgcggctg tttcattttt tagcgcgtga ctacattgtg caggaagggc
    26101 aacagccgtc ctggctgttt tacctgacgc gaggccgcgc caggctttac gccacgctag
    26161 ctaatggtcg cgtgtcgctg atcgatttct ttgccgcccc ctgttttatt ggcgagattg
    26221 agttaatcga caaagaccat gaaccgcgtg cggtgcaggc tattgaagag tgttggtgcc
    26281 ttgcgctccc tatgaaacat taccgcccgc ggttattaaa cgacacgcta tttttacgaa
    26341 aactctgcgt caccttaagt cataaaaatt atcgtaatat tgtttcttta actcagaatc
    26401 aatcatttcc gttagtgaat cgcctggcag catttatatt actctcgcag gaaggtgatc
    26461 tttatcacga aaagcatacg caagcggcag agtatttagg cgtttcttat cgacatcttt
    26521 tatatgttct cgcgcaattc attcacgacg gtttattaac taaaagcaag aaagggtatc
    26581 tcattaaaaa cagaaagcag ttgtcaggac tggcgctgga gatggacccg gagaataaat
    26641 tctccgggat gatgcagtaa aaattatttg caatagcgcg attgccggat gcaacgctta
    26701 atacgtttta tccggtctac aaatcgagca ttacgccaga ccaataaaga acccggcaat
    26761 agtcgcactc atcaggttgg aaagcgttgc tgctgccagc gcccgtaaac caagctgggc
    26821 gatttccggc gcgcgttttg gcgaaatagc cgaaaatgcg ccaacgacaa caccgataga
    26881 accaaagtta gcaaaaccac aaagcgcaaa ggagataatc gcaatggttt tcacttccag
    26941 cgtgccgccc gtttgcaggt atggggagaa actcaggtaa gcgacgaatt cgttaatcgc
    27001 cagtttctgc ccaatcaggc tacccgcaag attggcatca ctccagtcca cacccatgat
    27061 ccacgccagc ggtgccagca catagccaaa aatactttcc agagaggcat tggcgaaacc
    27121 aaaccagcca ccaattccgc cgataatgcc gttgatcagc gcaataattg cgacaaacgc
    27181 cattaccacc gtcgctacac cagcggcgat tttcagcccg gtcatcgcac cgctcgccgc
    27241 cgcttcgata aagctttttg gcggcgtttc gctgaacgac agattttcaa atgtgacctg
    27301 tgaaggctcg gtggccgggc taagaatacg tgcaaacaaa ataccacctg ggatcgccat
    27361 cagcgatgcc gctaacaggt agtcaattgg tacgcccatt ccggcataac caatcatcat
    27421 cgaaccagca atggacgcca tcccgctaca aattgcggta aacaactcgt tgcgattcat
    27481 gcgatcgata aacggtttaa cgatcgccgg gatctcattt tgcccgagga aaatagttgt
    27541 gactgcgaca aaagattcga ttttgctgat gttaagagct ttctggaaaa tgctgccaag
    27601 gatgcgaatc agcagcccca tcacgccaat gtagtacagc agactgatga gcgcagtaac
    27661 gaaaataatc gccggaagta cgcgaaaggc gaagataaaa cccgcaccgt caaacaggac
    27721 atccattttc ggcccaacca gcgaaccaaa aatgaaggcg ctaccggcat cactgtaaga
    27781 catcacttta tgaacgccta atgccgcctg ttctactgcc cactttcccg gtgggaagta
    27841 gagcatgatg ccaccaatag cgatttgcag cagcagtgcg gctccaaccg tgcgcaaact
    27901 gatgctcttt ttattcactg acaacagaaa tgctattgcc agtaacacca ccatccccac
    27961 aacacttctc attatatcca taatgatttt cccttcatgc cagtaaaccc ggcgtcagcg
    28021 ccaggttttg gtatgcttga tgagtacggg cgacggcttt ctgcccgtca gaaattaacc
    28081 cgcgagacgc tggtattctt tggcaatttc actcgccaga atggcgttgt tgaacaccag
    28141 ctggatattg gatttcaggc tgtcaccgcc ggtcagctca gcaacgcgcg ccagcaggaa
    28201 cggtgtactt tctttaccaa taacgccctg ctcttcggct tcggcgacgg cttgatctat
    28261 ggctgcattg atagattcct ccggcatcgc aaactgttcc gggatcgggt tcgctaccac
    28321 gaggccaccg ttcagcccgc tttgccattt caccgccatt gcacgggcaa tttcactggc
    28381 gctgtcgaga cgaatgctga cgtcaaacga gctggtacgg cagaaaaacg caggcagcgc
    28441 tttagtctga tagccaatta acggcacacc gaaggtttct aaatactcag tggttaatcc
    28501 taaatcgaga atagatttcg ccccggcaca aacaacggtg acattagtat ttgccagttc
    28561 ttgcaaatcg gcagaaatat cgaaggtatg ttccgccccg cgatgcacac caccaattcc
    28621 cccggtggca aatactttaa ttccggcaag cgccgcaata atcatcgttg aagccacagt
    28681 ggttgcgcca ttttttccgg cggcaacaac aaaaggtaaa tcgcgacgac taactttggt
    28741 cacgttatgc ccttcacgac ccagtaattc aatttcttct ttgcttaaac ccactttcat
    28801 cacgccgcca ataatggcga tagtggcagg tactgcgccc tgtttacgaa tagtttcttc
    28861 tacttcaatt gcggtctggg cattttgtgg gaacggcatc ccgtgagaaa taatggtcga
    28921 ttccagcgcc acaaccggtt ttttgttttt taaagcgtcc tgcacttccg gggaaatttg
    28981 taataattca ggggaaattt ttaattcaga cattctgcgt tctccactaa cgatataacg
    29041 ttggcaatcg ataaatcggg gttattggtg tattcacagg agagcgccat tgacgaacat
    29101 ccctgtgcga aacgaacaga ttcggcaaac ggcattccgt ctacccaaca cgaagcaagt
    29161 cccgccatca tggcatcgcc cgctccggta acattaataa cattggtttt gatcggcgca
    29221 gaccagccat tttcaccgct gatatcgctg taataaacgc catcgccgcc catgctcaat
    29281 accagtcggt tcaggccatg ttgatggaac caggcagcaa cttttgccac atcgtcacgc
    29341 cctgacagcg caatcccact cagggtttcc gcttcaaggc ggtttggctt gagagtgtgg
    29401 atctgattta gacggtcgcg cactttgaca catttccacg ctgaaaccgg atcgacaaat
    29461 acgggtacgt tggcggcatt atccagaatc catgccagtg cctcttcact gatattacag
    29521 tctgcgacaa tgacctttgc cctctgaata aattcacggt gctgtgcgag atattcagct
    29581 gtaatagcgt tgctaatatt catgtcattt atagcaacca gcatttcacc ggtattatcg
    29641 agtaatgata aataactaga cgtattttct cccggcacaa tcaggcattt atcgacataa
    29701 acgccagatt gattggtttg cgttagcagc gattgaccat aaaaatcact gcctactgcg
    29761 ctcagtagcc aggctttgtt acccagcaac gccaggtttt gtgcaatatt gcgccctact
    29821 ccaccaggcg taaattttat tttacctgga tttgaatccg cataatttaa tgattcatgt
    29881 gaatatccgg cgacatcaat atccggcgac atcaatattc gccgaaccta taattacgac
    29941 ataatccttt tcgcgcatag cacgtcctct ggtaaaaaat taccaaccct gtcaatccac
    30001 aagaatatag atactgcaaa gacggtttaa acatgtgttc atatttaaac atgtgctcat
    30061 agtatgcatg ttttacgtaa agtaaattac cgccagagag gcaaaatggg cagatttgag
    30121 caggctcaca aaatgcgatt aaatgcctga attacaaaag cgaggaattt gtgacgggat
    30181 gcacagaaca ggaacactaa aaaagggcag aaaataatct gcccttgagg aaataacctt
    30241 agtgtttgat gaacgtttca ggtttctact gaaagataaa accatttaaa ttcataaaat
    30301 tacatttaca aaccggtcat tccctttgcg ctcatccaat gatacagggc aggaaatttc
    30361 tgccctgtaa cacacctttt attacgctgc tttcgctact gcgtccactt ccggacgttt
    30421 caggaaggca taggccaaac ccgccaccag cgtaccggca ataattgcta ccaggtaacc
    30481 caataccggc gtaatagcgc cagggatcag cagaacaaac agaccaccgt gcggtgccat
    30541 cagtttcgca ccaatcgcca ttgagattgc gccagtcagc gccccaccca cgatacagca
    30601 cggcagcaca cgcatcggat cacgagcagc aaacggaatt gcaccttccg aaataaagca
    30661 cagtcccagt accagagcgg ctttgccacc ttcctgctgc gctttgtcga atttgcgacg
    30721 cgccaccatt gttgccaggc ccattgccag cggtggcacc atacctgccg ccataatcgc
    30781 cgccatcggg ccataggttt gagtactcag cagacccaca ccgaatgcgt acgctgcttt
    30841 atttaccgga ccgcccatgt cagtacacat catgccaccg aggatcgccc ccagcagaac
    30901 cgcattcgca gtccccatgg tctgcagcca gtgagtcaac ccttcgagaa tgccagcaac
    30961 cggtttaccg atcaggtaga tcatcgccag accgaccacc agactggaaa ttagcggaat
    31021 gatcaggatc ggtttcagcg cctccatact ctgtggcagt ttcaattgcg tactgattaa
    31081 cttcgcaatg taaccagcca ggaagcccgc aataatacca ccaatgaagc cagaaccggt
    31141 gctgaccgcc agcataccgc caatcagacc cggcgtgagg cccggacgat cggcaatgga
    31201 aaaggcaata taacctgcca gtaccggcac catcagcgca aaggctgaac caccaccaat
    31261 ctgcatcagc gccgctgcca acgtacccgg ctctttaaac gcttcgatac caaaagcaaa
    31321 agaaagcgcg atacacagac caccagcaac gaccatcggc agcatgtaag agacgcccgt
    31381 tagcaagtga cgataagcgc ctgcactctc tttcttacct tcagtggtcg ccgtttgagt
    31441 tttgcccgcc ggttcatacg gcgttgcttc agcaaccgct ttatccagtt cctgcgcggt
    31501 tttcttcagc gccagaccgg tagaggtacg atacatcggt ttaccagcaa atttcgccag
    31561 atccacttcg atatctgccg ccacaatcac cagatccgct gcggctactt cctcgggagt
    31621 gattgcatta cccgcgccaa cagaaccacg ggtttcaact ttcacccacc agccacgttt
    31681 tttcgcttcg gtttcaatgg cttcagccgc cataaaggtg tgtgctacgc cagtcgggca
    31741 agcagtcacc gcaactacgc gtttcggacc gctggcggca accggtgctg tcgcagtgac
    31801 cggcgcagtg taaggtttcg catggccttt ggcttcactc aggaacagct cagggtgcgc
    31861 aactgcccgg gaaatatcac ccagccagac atttttaccg ttcagcgcgc tgtcattcgg
    31921 gatggaatca ccgagaacaa tcgccatttc tgcgtcgttc ggattgtcga tgatttccag
    31981 ttttgctttt cgcgccgccg cgcccagcag ggtcttcgcc atataggcgc gtgcctgacc
    32041 gagattagcg tcaataatca gcagcgtttt cattatgcct ctcctgctgt cagttaaaag
    32101 gttgtaagtc gacgcgcgcc atcattgcgg ccaactgcgg acgatcggta atacccacat
    32161 tgctttgact taccgccagg gctgcaacag ctgtcgccag acgcagtgtg tgttcactgg
    32221 attcacgcat cagcaagcca taaatcaggc caccaaccat agaatcccct gcgccaacgg
    32281 tgcttacgac atcgactgac ggtggtttgg cgatccattc gccggaggca ttaacccaaa
    32341 gcgcgccttc ggcacccagt gaaataacaa catgcgcaat accttgttca cgtagcgcat
    32401 gtgcagcttc aatcacatct ttcatttcag gcagtttacg gcctgcccag atttccaact
    32461 cgcggcggtt aggtttcacc agccacggtg ccgctttcaa acctgctact aacgcttcac
    32521 ggctactatc aaagataatg caaggacact gactacgcag gcgagtcatc cagtcggtga
    32581 acgcttccgg gctgacgcct gacggtaagc ttccgctgac acagaccata tcgaactgac
    32641 cgagccagct cagagaatca gtcacaaagc gttcccagtc ggcgggggtg acttcaaaac
    32701 ccgagaagtt gaagtcggtc acttcgccgt ctttttccgt cagcttaacg ttaattcggg
    32761 tgcgcccctg tacaacctgg aaacggttgg caatgcccag ctcgctgaac agttgctgaa
    32821 aaccatcctg attgtcttta cccaggaagc cgccaacggt gacatcaatt cccaggtctt
    32881 ttaatacttt ggccacgttg atgcctttac ccgccgcatg cagaccggtg gttttcacca
    32941 ggttcacttc gccgcgttca atttccgggc agaaaccaac aaggtcataa gccggattaa
    33001 gggtgatagt agcaacacgt ctgctcatta tgcgccctcc ccaagaccag cagcgatagc
    33061 gtcgccgatt gctttcagcg cctgttcagc atctgcaccc tgggcggtaa agcgtaggcg
    33121 atgacctttc ttaacgccaa gtgccacaac tttcatcaga ctacgtccgt ttgccggttt
    33181 gccggtacca tcaaggtttg tcacggtaat atcactgtta aattgtttaa tggtattgac
    33241 cagcatggta cctggacgag catgcaggcc gtgttcattg cgcaccacaa actccgcgct
    33301 taacacgtcg tcggtcggcg catcatcgct ggtcagcagc gccagcaacg ttgccgcatc
    33361 cgctttcagc aagcggtcag ctttattgtc gagcaacaaa tcagcgagac gcttaagaac
    33421 cgcaatgggc tgatcgtcat tcatcgccac actcaccagc atggctgccg tttcgccgtc
    33481 cacatcaaaa gcatttgccg cacggcttac cgcaatcgcg ctacgcagat tgccttcggc
    33541 gctatcgctc agccagatac cctgtccgag gttcagcggt tgttcattga tggctttggt
    33601 gacgaaagtg gcgtcaactg ccctcgcctc tttcagacgc gcagcgttca gcgcctgaag
    33661 agtcagcaga tcgctggcga cgatatccag cgtcagcatt tcgttgtcga gcttcagctg
    33721 ctcactctgc ttttcgccca tcagtaatgc gcgaagttct tctgctgttg ttgctgactt
    33781 cagttgttca gcaacggaat catcgctcag tacgtgggtc agctggcgta gcaggcccag
    33841 atgttcatcc gagctggcag caataccgat tgccacgtac gctacctgac cgtcacccca
    33901 ggtgacgcct tccgggaact gaaatacctg aacgccggtt ttcagcacct gatcgcgggt
    33961 gtcggtagtg ccgtgtggaa tagcaatacc attgccgagg aacgttgagg tttgctgttc
    34021 gcgcgccagc atgccattga cgtagccttc tgctacatta ccggcctgca ccagcgccgc
    34081 agcgacctgg cgaatcgcct cttctttgtc tccggccttt tcgcccggat ggatgtcctg
    34141 tacggataac tggaacatag ttctcctctc ttgctgaatt gaaacgattc agcctctatg
    34201 agaaaaaaag cgccaacctg gcttagggtt aaagacaaga tcgcgctgaa acgtttcaag
    34261 aaagcataat acttctgttt cagcacgcaa ggaaagccgt aaagttatga gcaaaagttt
    34321 gatgtgctgc acaatttttt gccatttttc ctaattgctg atgggaaaat ccgttatcag
    34381 caatttcatt tcagcagcgt cagcagccaa tcagcaacag tgaaatggca ttttgattat
    34441 tcgtggcgct tttgatttcg ctgtctgttt attttctgac aagcagcgta aactccgcgt
    34501 cttcctcttc cagtgatcga ccagcatgca taactccccc gcagtctcca gcgcgaaatc
    34561 gtttgacctg acctcgacgg cgtttttaat cgttgccttt ctcaccggta ttgcgggcgc
    34621 tctgcaaacc ccgacactca gtatttttct tactgatgaa gtacatgctc gtccggcgat
    34681 ggtgggattc ttctttaccg gcagcgctgt cattgggatt ctggtaagtc agtttctcgc
    34741 cgggcgctct gataagcgcg gcgatcgcaa atcgctgatt gtcttttgct gcctgttagg
    34801 cgtgctggcc tgcacccttt ttgcctggaa tcgcaactac tttgttttgc tattcgttgg
    34861 cgtctttctt agcagctttg gctcgaccgc taacccgcaa atgtttgccc ttgcccgtga
    34921 acatgccgac aaaaccggac gtgaggcggt gatgttcagc tcttttttac gcgctcaggt
    34981 ttcactggca tgggtcattg gcccaccgct ggcttatgcc ttagcgatgg gtttcagctt
    35041 tacggtaatg tatctgagcg cagcggtagc gtttattgtt tgcggtgtga tggtgtggct
    35101 gtttttaccg tcgatgcaaa aagagcttcc gctggcgacc ggcacgatcg aagcgccgcg
    35161 ccgtaaccgt cgcgatacgc tgctgctgtt tgtcatttgt acattgatgt ggggctcgaa
    35221 cagcctgtac atcatcaaca tgccgctatt tattatcaac gaactgcatc ttcccgagaa
    35281 actggccggt gtgatgatgg ggaccgccgc cgggctggaa atcccgacga tgttgattgc
    35341 cggatatttc gccaaacgtc tgggtaagcg tttcttaatg cgcgttgctg ccgtgggtgg
    35401 cgtctgtttt tacgcaggaa tgctgatggc gcattcacct gtcattctgt tgggcttgca
    35461 gctgctaaat gctattttta ttggcattct gggcggcatc gggatgctct attttcagga
    35521 tctgatgccc ggtcaggcgg gttcagccac cacgctctat accaacacgt cgcgcgtggg
    35581 ctggatcatc gcaggatcag tggcgggcat cgtcgccgag atctggaatt atcacgctgt
    35641 gttctggttt gcgatggtga tgattatcgc cactctgttt tgcttactgc ggattaaaga
    35701 tgtttaaggc gcggtcagca tttccagatc gataagccag gtcatggcct gctgacgcga
    35761 gttaccgcac atttcagcgc tggcttgtaa gcctgcacag actttcggtc gcagtggcga
    35821 ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg cagggcgtat tggcgggctt
    35881 gccatctggc ataccgggaa tagggctgga aattgaaggg gcagtgcaac acgccccgca
    35941 acccagacgg catttcatac gttgttctct taaggttaac caccggattg atgaacgcgc
    36001 acagtaccac cttttttgca ccagcaaaag tgcgaatacc acttgcccga aaggcccgtc
    36061 gcgagtactt tgtcgcgata tttttgacat tttcgactac aggaattttt cgatgccaag
    36121 agcgaacgaa attaaaaaag gtatggtact gaattacaac ggcaaactgc tgctggttaa
    36181 ggatattgat attcagtcgc ccactgcccg cggtgccgct acgctgtaca aaatgcgttt
    36241 ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc aaaggtgatg atatcgttga
    36301 caccgtgacg ctgacccgcc gttacgttga cttctcctat gtcgatggca acgaatatgt
    36361 ctttatggat aaagaagact ataccccgta taccttcacc aaagatcaga ttgaagaaga
    36421 gttgctgttt atgccagagg gcggcatgcc ggacatgcag gtgctgacct gggacggtca
    36481 actgctggcg cttgagctgc cgcagaccgt tgatctggaa atcgttgaaa ccgcaccagg
    36541 catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg acattgagca ctggtctggt
    36601 gattcaggta ccagaatact taagcccggg cgaaaaaatt cgtatccata tcgaagaacg
    36661 ccgttatatg gggcgtgctg actaacttca gccgcatgca gaaaagggat agctcaggct
    36721 gtcccttttt taatttatta taccaatctt ctattttgcg cttcctgaac accttacgcc
    36781 ctcaattttc actcgttgat tgatcgccct cacacttcat cgcattaaca atccagacca
    36841 atttcaattg ctgtcatata actttacact gtcattgtta atttatcgtt actaagacgt
    36901 gactcctatg aagacaattg cctccgttac gctcccgcat catgtacacg ctccacgcta
    36961 cgatcgccag cagttgcaat cacgtatcgt tcattttggc tttggcgcct ttcaccgcgc
    37021 tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag ggcggcgact gggggatctg
    37081 tgaaatcagc ttgttcagcg gtgatcaact gatgagccag ctccgcgcac agaaccattt
    37141 atataccgtg ctggaaaaag gtgcggacgg caatcaggtg ataattgtcg gtgccgttca
    37201 cgaatgcctt aatgcaaaac tggattcctt agcggcaatt attgagaaat tttgcgagcc
    37261 acaggtggca attgtttccc tgacgattac cgaaaaaggc tattgtattg acccggccac
    37321 cggtgcactc gacaccagta atccgcggat tattcacgat ctacaaaccc ctgaagaacc
    37381 tcactccgca ccgggtattc tcgtcgaagc actgaaacgc cgccgtgagc gcggccttac
    37441 accgtttacc gtgctctcct gcgacaatat tcccgacaat ggccatgtgg tgaaaaacgc
    37501 ggtgctggga atggcagaaa aacgttcgcc agaactcgcc gggtggataa aagaacacgt
    37561 cagttttccg ggaaccatgg tcgaccgcat tgttccggct gcaaccgacg aatcactggc
    37621 agaaatcagc cagcatctgg gggtaaatga tccctgcgcg attagctgcg aaccgtttat
    37681 ccagtgggtg gtggaagata acttcgtcgc tgggcgtcct gcctgggaag tcgcaggtgt
    37741 acaaatggtg aatgatgtcc tgccatggga agagatgaaa ctgcgtatgc ttaatggcag
    37801 ccactctttt ctcgcttatc tgggttacct ttcaggattc gcccatatca gtgattgcat
    37861 gcaggatcgc gcatttcgcc atgccgccag aacattaatg ctgaatgagc aagcgccgac
    37921 actacaaatt aaagatgtcg atttaacaca atatgcggac aagttaattg cacgttttgc
    37981 taatccggcg ctgaaacata agacctggca aatcgcaatg gatggtagcc agaaattacc
    38041 gcaacgtatg ctggcaggta ttcgcataca tctggggtgc gaaacggact ggtcgttgct
    38101 ggcattaggc gttgctggct ggatgcgtta cgtcagcggc gttgatgatg ccggaaatgc
    38161 cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa cttgttgcgg gcagcagcag
    38221 tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt ttcggtgatg atctgccaga
    38281 taacccgcat tttgtgcagg ccatcgaaca agcctggcaa caaatcgtac aattcggcgc
    38341 acatcaggcg ctattaaaca ccctcaaaat ttaacgattt ctgcggttaa agcggatgaa
    38401 gctcaccttc gtccgctctc cccttctctt ttctgccttt tttagccagg attaacgctc
    38461 agttaactta ccagagtatt gcggagccgt tgtgaccagg accaacctca tcaccggttt
    38521 tctcggcagc gggaaaacca cgtcgattct tcatctgtta gcccataaag atcccaacga
    38581 aaaatgggcg gtactggtta atgaatttgg ggaagtcgga attgatggtg ctttgctcgc
    38641 cgacagcggc gcattgctga aagagatccc cggcggctgc atgtgctgcg ttaatggttt
    38701 acccatgcag gtagggttga ataccttact gcgtcaggga aaaccagacc gcttgttgat
    38761 agagccgacc gggctgggcc atccgaaaca gatcctcgat ctcttaaccg caccagtcta
    38821 tgaaccgtgg atagatctgc gcgccacgtt gtgcattctc gatccacgcc tgctgctgga
    38881 cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg gctgccgcag acatcattgt
    38941 cgccaataaa tccgaccgtg cgacacctga aagcgagcaa gcgctacagc gctggtggca
    39001 gcaaaatggt ggcgatcgac aattaattca cagtgagcat gggaaagttg acggtcatct
    39061 tctggatttg ccgcgtcgca atttagccga gttgcccgcc agcgccgcgc attctcatca
    39121 gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca gagcatcaac gctggcgtcg
    39181 cagtctgaac agcgggcaag gatatcaggc ctgcggctgg atattcgacg ctgatacggt
    39241 attcgacacc attggcattc tggaatgggc gcgacttgca ccggtggaac gcgtcaaagg
    39301 cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt cagggcgatg acctgcacat
    39361 tgaaacgcaa aacgttgcgc caccggacag ccgtattgag ctgatttcca gcagcgaagc
    39421 tgactggaat gctttgcaga gcgcgctgtt gaagcttcgt ttagcgacta ccgcgtaagg
    39481 ttgcctgcgt ttttcagtaa gataattaga gaaaatatga ttaaaaattt gccgcaaata
    39541 gtgttgttga atattgtcgg cctcgcgctg tttctttcct ggtatattcc cgttaatcat
    39601 ggattctggt tgccaattga tgcggatatt ttttatttct ttaatcagaa actggtcgaa
    39661 agtaaggcct ttttgtggct ggttgcattg accaacaatc gcgctttcga cggttgttct
    39721 ctgctggcga tgggaatgtt gatgctgagt ttctggctga aagaaaacgc ccctggcaga
    39781 cgacgtatcg tgattatggg tctggtcatg ctattaactg cagtggtatt aaaccagctg
    39841 ggtcaggcat taattcctgt aaaacgggcc agcccaacat tgacttttac cgatattaac
    39901 cgcgtcagcg aactgctctc tgttcccacg aaagatgcct cacgagatag ctttcccggc
    39961 gatcacggca tgatgctgct tattttttcg gcattcatgt ggcgttattt cggcaaagtt
    40021 gcaggcctta tcgcccttat tatttttgtg gtttttgcat ttcccagagt aatgattggc
    40081 gcacactggt ttactgacat cattgtcggt tcgatgaccg tgatattgat cggtttgccc
    40141 tgggtgttgc tgacgccatt aagtgatcga ttaatcacct tttttgacaa atcactaccg
    40201 ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa ttaacatcat caggcttatt
    40261 tttatagtga ggataatcct gatgatgcgc accgtgcttt catccatcgc gcgcaaaaat
    40321 cattctctaa gtaaatgaat ggattgcatg cgtttcactc aatcgtactt taattgacca
    40381 atcccgctta ttaactttct gtatcacttt ttcttataaa aaatcatgta aaaccgctcg
    40441 ccaagaccgc accaatcggg taatctcgaa ctcgttttgc ctcggcggta gattatcctc
    40501 acagcatata attttgtgcg ttagtccaca gatttggcct taaggaattg tttcaacatg
    40561 cccaggtaat tagtctcgtg tcgcttggca tttttttata acgatatttg tcgttaagga
    40621 cttcaaggga aaacaaacaa catggtcaaa tctcaaccga ttttgagata tatcttgcgc
    40681 gggatccccg cgattgcagt agcggttctg ctttctgcat gtagtgcaaa taacaccgca
    40741 aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa catcatcact gcaagcttct
    40801 caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa aatcgcgaat tatggatcag
    40861 tatgctgact ggaaaggcgt acgttatcgt ctgggcggca gcactaaaaa aggtatcgat
    40921 tgttctggtt ttgtacaacg tacattccgt gagcaatttg gcttagaact tccgcgttcg
    40981 acttacgaac agcaggaaat gggtaaatct gtttcccgca gtaatttgcg tacgggtgat
    41041 ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg gtatttatat cggcaacaat
    41101 cagtttgtcc atgcttccac cagcagtggt gttattattt ccagcatgaa tgaaccgtac
    41161 tggaagaagc gttacaacga agcacgccgg gttctcagcc gcagctaata aaccgtttgg
    41221 atgcaatccc ttggctatcc tgacgagtta actgaaagca ctgcttaggc agtgcttttt
    41281 tgttttcatt catcagagaa aatgatgttt ccgcgtcttg atccaggcta tagtccggtc
    41341 attgttatct tttaaatgtt gtcgtaattt caggaaatta acggaatcat gttcatacgc
    41401 gctcccaatt ctggacgtaa gctcctgctt acctgcattg ttgcaggcgt gatgattgcg
    41461 atactggtga gttgccttca atttttagtg gcctggcata agcacgaagt caaatacgac
    41521 acactgatta tcgacgtaca aaagtatctc gatacctatt ttgccgacct gaaatccact
    41581 actgaccggc ttcagccgct gaccttagat acctgccagc aggctaaccc cgaactgacc
    41641 gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc tggtgaaaga taaaaaaaca
    41701 ttctgttcat ctgcgactgg tgagatggac attccactca atgaattgat tccggcgctc
    41761 gacattaata aaaacgtcga tatggcgatc ttacccggca cgccgatggt gccgaacaaa
    41821 cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa atagcggcgt ctttaccgct
    41881 ctgaatctca acctgacacc gtcactcttt tatagttcac ggcaggaaga ttacgatggc
    41941 gtcgccctca ttattggcaa tactgcgcta tctacctttt cttcacgttt gatgaacgtt
    42001 aacgaattaa ccgacatgcc agtccgtgaa actaaaattg cgggcattcc tctgaccgtt
    42061 cggctttatg cggatgactg gacatggaac gatgtgtggt acgcattttt actgggcggc
    42121 atgagtggaa ctgtcgttgg cctgctctgc tattacctga tgagcgtacg tatgcgcccc
    42181 ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt acgtggcgta tcaaccggtg
    42241 gtggatacac aagctttgcg agtaacgggc ctggaagtac tgctacgctg gcggcatcct
    42301 gtcgcgggag aaattccccc ggatgccttc attaactttg ccgaatcgca aaagatgatt
    42361 gtgccgctga ctcagcacct gtttgagtta attgcccgcg atgccgcaga attagaaaaa
    42421 gtgctgccgg taggcgtcaa atttggcatt aacattgcgc cggaccatct gcacagcgaa
    42481 agctttaaag cagatatcca gaaactgctc acttccctac ccgcacacca tttccagatt
    42541 gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag aagccacaca actcttcgcc
    42601 tggctgcact cggtcggcgt agaaattgct attgatgact tcggcaccgg gcacagcgcg
    42661 cttatctatc ttgagcgttt tacgctcgat tatctgaaaa ttgaccgtgg atttatcaac
    42721 gccatcggta cggaaacgat cacttcaccc gtacttgacg cggtgctgac gctggcgaaa
    42781 cgcctcaata tgctgacggt tgctgaaggg gtcgaaacgc cggaacaagc gcgatggcta
    42841 agcgaacgcg gcgttaattt catgcaaggc tactggatta gtcgcccgtt accgctggac
    42901 gattttgtcc gctggctgaa gaaaccgtat acgccgcagt ggtaaggtgt gcttacgtcc
    42961 cttattattc atagtgaaag catgccggat tgcggctaat gatgagtaaa aggaaatccg
    43021 ttgcagatga ttgtgcgcat actgctgctg tttatcgctc tgttcacctt tggtgtgcag
    43081 gcgcaggcta tcaaggaaag ctatgccttt gccgtgctgg gcgaaccccg gtacgcgttt
    43141 aatttcaacc attttgatta tgtgaacccc gccgcgccaa aaggtgggca gataacgttg
    43201 tcagccctcg gcaccttcga taatttcaac cgctatgcac tgcgcggcaa cccgggcgca
    43261 cgcaccgagc agctgtacga cacgctattt acgacttccg atgacgaacc aggcagttat
    43321 tacccgctga ttgctgaaag cgcacgctat gctgacgatt attcctgggt ggaggtcgct
    43381 attaatccgc gcgcccgttt tcatgatggt tctcccatta ctgcccgcga tgtagagttt
    43441 acttttcaaa aatttatgac cgaaggcgtg ccgcaatttc gtctggtcta caaaggcacc
    43501 accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt tagctaaacc cggcaaagaa
    43561 gatatgttga gtctgttttc gctgccggta tttccagaaa agtactggaa ggatcacaaa
    43621 cttagcgatc cgctcgccac gcctccgctt gccagtggtc cgtaccgaat tacgtcctgg
    43681 aaaatggggc aaaatattgt ctattcccgc gtgaaagatt actgggcagc aaacttaccg
    43741 gtaaaccgtg gacgctggaa tttcgacacc attcgctacg attattacct cgatgataat
    43801 gtcgcctttg aagcgtttaa agccggtgcc tttgatttgc gtatggaaaa cgacgctaaa
    43861 aactgggcca cgcgttatac cggtaaaaat ttcgataaaa aatacatcat caaagatgag
    43921 caaaagaacg aatcagccca ggatacgcgc tggctggcgt ttaatatcca acgtccggta
    43981 ttcagcgatc gccgggtgcg ggaagctatc actctcgcct ttgactttga atggatgaac
    44041 aaggcgttgt tttacaatgc ctggagtcgc acgaacagtt attttcagaa taccgaatac
    44101 gcggccagaa attaccccga cgccgcggag ctggtgcttc tggcaccaat gaaaaaagat
    44161 ctaccgtcag aagtcttcac acaaatctac cagccgccgg tatctaaagg cgatggctac
    44221 gatcgtgaca acctgttaaa agccgacaaa cttctcaacg aagcgggctg ggtgctgaag
    44281 ggtcagcaac gcgttaatgc cacaacgggt cagccactca gctttgaatt attgcttccc
    44341 gcaagcagca atagtcagtg ggtattgccg ttccagcaca gcctgcaacg gctgggtatc
    44401 aacatggaca ttcgcaaggt ggataactcg caaatcacta accgcatgcg cagtcgcgac
    44461 tatgacatga tgccgcgcct atggcgggcg atgccgtggc ccagttccga tttacagatt
    44521 tcctggtcat cggaatatat caattccact tataatgccc ccggcgtgca aagcccggtt
    44581 atcgactcgc tgatcaacca aattattgcc gcgcagggaa ataaagaaaa attactgccg
    44641 ttggggcgag cattggatcg cgtattaacg tggaattatt acatgctgcc aatgtggtac
    44701 atggcggaag accgtctcgc ctggtgggat aaattctccc aacccactgt acgccctgtt
    44761 tacagcctgg gtatcgatac ctggtggtat gacgttaata aagcggccaa actgccgtca
    44821 gccagacaac agggagagta gatgggcgct tacctgattc gccgtctgtt gctggtgatc
    44881 ccaacattat gggcgattat caccatcaac tttttcatcg tgcaaattgc gcctggcggt
    44941 ccggtcgacc aggccatcgc cgccattgag tttggtaatg ccggagtatt acccggcgca
    45001 ggcggtgaag gtattcgcgc cagccatgcg caaacgggcg tcggcaatat cagcgacagt
    45061 aattaccgtg gcggacgcgg attagatcca gaagtgatcg ccgagatcac tcatcgctac
    45121 ggtttcgata agccgatcca cgaacgttac ttcaaaatgc tctgggacta catccgcttt
    45181 gattttggtg atagcctttt tcgcagcgcc tcggtgctga cgctgattaa agacagtctg
    45241 ccggtttcca tcaccctcgg attgtggagc acgctgatta tctatctggt gtcgattccg
    45301 ttaggcattc gcaaagctgt ttataatggg agccgctttg acgtctggag tagcgcattt
    45361 atcatcatcg gctacgccat tccggccttt ttgtttgcca tcctgctgat tgtcttcttc
    45421 gcgggcggca gctatttcga cctgttccct ctacgcggcc tggtttccgc taactttgat
    45481 tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc atatcacgct gccggtgctg
    45541 gcgacagtga ttggtggctt tgcggcgctg accatgctga ccaaaaactc attccttgat
    45601 gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag gggtaagtga aaaaaatatt
    45661 ctctggaaac atgtgttccg caacgccatg ctgctggtga ttgccggttt tccggcgacg
    45721 tttatcagca tgttttttac cggctcgctg ctgattgaag tgatgttttc actcaatggc
    45781 cttggcttac tgggctacga agcgaccgtc tcgcgcgatt atcctgtaat gtttggtacc
    45841 ttgtatattt tcaccctgat tggcctgctg ctgaatattg tcagtgatat cagctatacg
    45901 ctggtcgatc cgcgtataga ttttgaggga cgttaatgtc gcgactcagc cccgtcaatc
    45961 aggcccgttg ggcgcgtttt cgccataacc gtcgcggcta ctggtcgtta tggattttcc
    46021 tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc caacgataaa ccgttgctgg
    46081 tacgttatga cggcagttgg tatttcccgt tgttgaaaaa ctacagcgaa agcgattttg
    46141 gcggcccgct ggcaagtcag gctgattatc aggacccgtg gctgaaacaa cggctggaaa
    46201 ataacggctg ggtactgtgg gcaccgattc gctttggtgc taccagtatc aactttgcta
    46261 ccgataagcc cttcccttct ccaccctccc ggcaaaactg gctgggaacg gatgccaacg
    46321 gcggcgatgt gctggcacgc attctctatg gcacgcggat ctcggttctg tttggcctga
    46381 tgctgactct ctgttccagc gtgatgggcg tgctggcggg ggcgctacaa ggctattacg
    46441 gcggtaaagt cgatctctgg ggacaacgct ttattgaagt atggtcgggg atgccgacgc
    46501 tgtttttgat tattttactt tccagcgtcg tacagcctaa cttctggtgg ctgctggcaa
    46561 ttactgtctt gtttggctgg atgagtctgg tcggcgtggt gcgggcggag tttttacgca
    46621 cccgtaattt cgactacatt cgtgcggcgc aggcgcttgg cgtcagcgat cgcagtatca
    46681 tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct caccttttta ccgtttattt
    46741 tatgtagttc gataaccacc ctgacctcac tcgatttcct cggcttcggt ctgccgctcg
    46801 gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa taaccttcag gctccgtggc
    46861 ttgggatcac cgccttcttg tcggtggcga tattattgtc tttgctgatc tttattggtg
    46921 aagccgtccg cgacgcattt gatcctaata aggcggtgta gcatgacgca aactctgtta
    46981 gcgattgaag atttgtcggt gggttttcgc catcagcaaa ccgtacgtac agtagtcaat
    47041 gatgtttcac tacagattga ggctggcgaa acgctggcgc tggtgggtga gtcaggttca
    47101 ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc cttccccgcc ggttgaatat
    47161 ctctccggcg atattcgttt tcgtggcgaa tcgctgcttc atgccagcga tcaaacgttg
    47221 cgcggtgtac gcggtaataa gatcgccatg atttttcaag agccgatggt gtcgttaaat
    47281 ccattgcata ccctggaaaa acagctttat gaagtgcttt cactccaccg cgggatgcgt
    47341 cgggaagcgg ctcgaggcga aattcttaac tgccttgatc gcgttggtat ccgccaggcg
    47401 gcaaaacggc tgacagatta tccgcatcag ctctccggcg gcgaacgcca gcgggtgatg
    47461 attgcgatgg cgctgttaac gcgaccggaa ttattaattg ccgatgaacc gaccaccgca
    47521 ctggacgtct ctgtccaggc gcagatttta cagctgttgc gcgaactgca aggcgagctg
    47581 aatatgggca tgctgtttat tactcataac ctcagcattg tcagaaaact ggcccaccgc
    47641 gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata acgccgctac gctatttgcc
    47701 tcacccactc atccttacac acaaaagcta ctcaacagtg aaccgtcagg cgacccggtg
    47761 ccgttgccag aacctgcctc aacgttgctg gatgttgaac agcttcaggt tgccttcccc
    47821 attcgcaaag ggattttgaa gcgcattgtg gatcataatg tggtggtgaa aaacatcagt
    47881 tttacgctac gagcgggtga aacactgggt ttagtgggcg agtccggttc cgggaaaagt
    47941 acgacgggac tggcgctgct gcgactgatt aattctcagg gcagcatcat ctttgacggt
    48001 cagccactgc aaaatttaaa tcgccgccag ctgttaccta ttcgtcatcg cattcaggtg
    48061 gtatttcagg atccaaactc ctcgctcaac ccacgactca acgttttgca gattattgag
    48121 gaaggcttac gggttcacca gccgacgctt tctgccgcac aacgcgaaca acaagtgata
    48181 gccgtgatgc atgaagtggg attagatcct gaaacacgcc accgttatcc ggcggagttc
    48241 tctggtggtc agcgacaacg tattgcgatt gccagggcat taattcttaa gccctcgctg
    48301 atcatacttg atgaaccgac atcatcactc gacaaaacgg tacaggcgca aatattgacg
    48361 ctattgaaat cattgcaaca aaagcatcaa ctggcctatt tgtttatcag ccacgatttg
    48421 cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc gacaagggga agtagtggaa
    48481 caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg agtatacgcg tcagctactg
    48541 gcgttgagct gacgcttaaa aaggattgta gtttgaaaag ggttcggcaa ttgccacacc
    48601 aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg cgatttacaa acaggcacgg
    48661 ctcaccttca cactccagca ccgaacatgg aacttcgatg tcgcttaacg cctggcttag
    48721 tttatacatc accgaccatg cattatcacc atccgggcta agcaatttaa gcgccaccag
    48781 gctgttgtca ccgcacggtc cattttgcgg aatcggttca accttcgaac ctgcgaaacc
    48841 agcagaccag cgatagttct gcggcagtcg atggacgatt gagagttgta atgaagtcac
    48901 ttattttccc cggaagcaca ttcacttcac aatttgttta catcaatttt aacacatcat
    48961 caacaatccg tctttataca gatcgtaaag attgatgcct cgcctgcgtg gcctctatgg
    49021 ctctgattta agtaatatct gcgggcgtgg tcacgtttgc ggggctatat gtacccgttt
    49081 ctgcgatcta actcaacctt tttaactaca atgatgtgac tttttacata aattgatttt
    49141 acataaaata aacatatatc gggggaatga tagatttgtg gttgacatgc atcaacaaag
    49201 gaagcctttt agcttcctcg ttgtgcaata gatcaccgtt ttttcggtcg actggcgtac
    49261 agacagaaga gaatggagct gcttgcgcag aatgcaattg accaaatcat cggccatgca
    49321 gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg cccctatgcc aaaacggaag
    49381 gttcctgcca gcgaagatgc cgttcccgcc atatggggaa actcatcaag aatgaccgcc
    49441 atcgcattgg atgacaccat cgacacgcag cccacaaacg ccgcaacgcc aaccaccagc
    49501 gaccagaatc ccagccccag cagcgcactg atgaccatcc acgctgccat aataaattgt
    49561 atccacaacc ccgagcggaa catatttaac gcgccaatgc ggcgaacgaa gcggctgtta
    49621 aagatggtca tcacgaacag gaaaacaatg tttagtgcaa agtaataacc aaagttttcc
    49681 ggcgcgacgt ggttaatttc aatgtagaca aacggtccgg cgctcaggaa cgagaacatt
    49741 ccggcaaagc tgaaaccgct ggccagcata taactcagga cacgcttatg acggaacagc
    49801 gccgcaaagt taccaatagt ggtacgaatg tgaaatggct gacgacgctc cggtggtaag
    49861 gtttctttaa tcaggaagaa aatcattgcc gaagccagaa tcgccgctaa tgccaggatc
    49921 cagaagatgt aatgccagct cagccacacc agcacccaac cgccgacaat cggtgccatc
    49981 aacggtgcaa tagtggtcac cagcatgaca aacgacatca tccgcgagaa ctcttctttc
    50041 gggtaaatat cgcgcatcag ggcgttaatg accacgctgg ccgcagccgc agccagcccg
    50101 tggaagaaac gcatcacaat cagctgatcg atggtgtttg ccaacgcaca cgccaccgcg
    50161 gcggcggcaa acaccagcgt accgccgagc accaccggct tacgcccgaa gctgtctgcc
    50221 atcggcccgt agattaactg ccccaacgca aagcccagaa tataagtact gagggtcatc
    50281 tgcgtactgc ccgcaggtac gccaaactgc gctgaaatta ccggtagcgc gggcagatac
    50341 atatcaatcg acagcggcat caacatggcc agcaggccaa ggataaaaac aatagcaaac
    50401 gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt catggaaaag ttagacgacg
    50461 ctggcaattt cttcttcagt taacggacga tattcaccgg gggctaaatc agcatccagc
    50521 gtaataccgc caatacgttc acgatgcagc tcaaccacat ggttacccac agcggcgaac
    50581 atgcgtttca cctgatgata acgcccttcg ctgatggtca gacgaacctg cgttggggta
    50641 atcacttcca gcaccgcagg cttagtgaga tctttttcgt tatgcagctg tacgcctttg
    50701 gcaaattgct ctgccgtatc gtcagctaca ggtgattcca gtgtcaccag ataggtcttc
    50761 tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact gaccatcatc agtcatcagc
    50821 accagaccgg tggtatcaat atccaaccgc cccgccgcat gcagtttcca cgctaccggt
    50881 tcatcaagaa aatagagcac cgttgggtga tcagggtcgt ccgtggagca aacatagccc
    50941 tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct gcgccagcgg gttgccatcg
    51001 taagcgacat catgttcagg aagcagtttg aacgctgcat tacggacgat ttcgccatcg
    51061 acggtgacac gattgccgcg gatttcacgc ccggcaatag cacggctaac gccgagttgc
    51121 tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc ctgtaaaaac ggaggtcggg
    51181 cattgacccg aaaatctgaa ctgttgtctg cccagtatag cggtctaata acatccctca
    51241 agggaaaaag atccatggca tactattagc agaataatct acctacgcga gaccatgatt
    51301 tttacacttc gcccatatca gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt
    51361 cataaaaccc ctgccgttat cgtgctgccc accggcgcag gtaaaagcct ggtgatagcg
    51421 gaactggcac ggctggctcg tggtcgcgtg ctggtgctgg cacacgttaa agaactggtg
    51481 gcgcaaaacc atgcaaagta tcaggcgctg gggctggaag ccgatatttt tgccgctggg
    51541 ttaaagcgca aagagagcca cggtaaagtg gtatttggca gcgtgcagtc ggtcgctcgt
    51601 aatcttgatg cctttcaggg tgaattttcg ctgttgattg tcgatgaatg ccaccgtatt
    51661 ggtgacgatg aagagagcca gtatcagcaa atcctcactc acctgactaa agtgaatccc
    51721 cacttacgcc tgctggggct gactgccacg ccttttcgat tgggcaaagg ctggatctac
    51781 cagttccatt atcacggcat ggtacgcggc gatgagaaag cccttttccg tgactgcatt
    51841 tatgagctgc cgctgcgtta tatgattaaa cacggctatc tgacgccgcc agaacgactg
    51901 gatatgccag tagtgcaata cgatttcagc cgcttgcagg cacagagtaa cgggctgttc
    51961 agcgaagctg atctcaaccg tgagctgaaa aaacagcaac gcattacgcc acatatcatc
    52021 agccagatca tggagttcgc cgaaaagcgc aaaggggtaa tgattttcgc cgccaccgtt
    52081 gaacacgcaa aagagattgt gggattactg cctgccgaag atgctgcact gattactggc
    52141 gacacccccg gcgctgagcg cgatgtgtta attgaagatt ttaaagccca gcgttttcgc
    52201 tatctggtca acgtcgcagt actgaccacc ggatttgacg ccccgcacgt cgatcttatc
    52261 gccattctgc gccctaccga atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg
    52321 cgtctcgctc cgggcaagac tgattgttta attcttgatt atgcgggtaa tcctcacgat
    52381 ctctacgcgc cggaagttgg tacaccgaaa ggcaaaagtg acaacgttcc ggtacaggtt
    52441 ttctgccctg cctgcggttt tgccaacacc ttttggggga aaacgaccgc cgacgggaca
    52501 ttgattgaac actttggtcg ccgctgtcag ggatggtttg aagatgacga cggtcatcgc
    52561 gaacagtgtg acttccgttt ccgttttaaa aattgcccgc aatgtaacgc agaaaatgat
    52621 attgccgctc gccgctgccg cgaatgtgac acggtactgg ttgatccgga tgatatgtta
    52681 aaagcggcgc tacgactgaa agatgcgctg gtattacgct gtagcggcat gtctttgcag
    52741 catgagcacg acgagaaagg cgaatggttg aaaatcacct attacgatga agacggcgcg
    52801 gatgtgagtg agcgtttccg tctgcaaacg cccgcccagc gaactgcctt cgagcagctt
    52861 tttatccgcc cgcatacgcg cacaccgggc atccctctgc gctggatcac cgccgccgat
    52921 atcctcgccc agcaagcctt attgcgacac ccggattttg tcgttgcccg catgaaaggt
    52981 cagtactggc aagtgcgtga aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac
    53041 gaattacgcg gttaatggta gttttgattg atgtataaag cgattgagta tagaatctcg
    53101 cccgcttttg catacgcaaa gcagatcact tacctgttgc tgggtcgcct gtagcaggat
    53161 taatttaaga gagaaagaaa tgtttactat caacgcagaa gtacgtaaag agcagggtaa
    53221 gggtgcgagc cgccgcctgc gtgccgctaa caagttcccg gcaatcatct acggtggcaa
    53281 agaagcgccg ctggctatcg agctggatca cgacaaagtc atgaacatgc aagctaaagc
    53341 tgaattctac agcgaagttc tgaccatcgt tgttgacggt aaagaaatca aagttaaagc
    53401 tcaggacgta cagcgtcacc cgtacaaacc gaagctgcag cacatcgact tcgttcgcgc
    53461 ttaattgctg aataagttgt aaaaaacccc gctccggcgg ggtttttgta tctgcagatt
    53521 atgcctgatt acggtattgc tattttttgc aggccagata aggcgttcac gccgcatctg
    53581 gcattcagaa cacaaaaccg attaattgcc gccagacgtc cggcgctgca attggtcacg
    53641 caaattcggc ggcgtgcctt taatggtcag ggtatcggtt gccggatccc agaagatgcg
    53701 ttcacctaac agcatcgcat caaagttaat agtcaagcca ccgccgctac cggcaaactt
    53761 cgtcaactga cgcagtgtgc tacggtctgc cgggaagctc tcttccagtt cgtagccttt
    53821 ttcagccgca aactccgtga aactcacttc gctgacgcct gccagctctt tcgacagcga
    53881 ctccagttcg atctcttcgc ctgcctgcag ctgctcatta cagtagctat aaacctgctg
    53941 gcgcacgttc tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat catcaaccgc
    54001 ttgcaacagc ccacggtttt gcgctttcgc gtttaaccct tcgctggccc cgaggaaatc
    54061 cataaagaaa tccgccactt tgcgccctac tcgccctttc aggaaagtga gatagcgggt
    54121 ggactctgga ttggtttccc attcggttaa atcgatacgc gcaacaatat ccgcatgatt
    54181 gatatcaaga taatgggttg gattgatgtc gagattttcg ttaacgcgca tactgctcag
    54241 gttgctcaga actgccacca gcagatactc caccgccaga taacggtagt ggcaaaacag
    54301 cacgaatcca ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc gcccggttgc
    54361 cgcgcggcta aatgccagaa agtcctcttc gccctgacgc tgtaagcgca gcgtctgcgc
    54421 cagttcactc tcttcgctaa acaagccgta agctttattt ttggcgctat agacccgatg
    54481 cagttctgcc accatctcaa cgacggtttc tgtcggttcc agcaatgaat cgcgcaacac
    54541 cagctcaagg ttctgctcat cacgcttgat aagctggtgc agagcaatct ggttgatatc
    54601 cagactcatg ataaactctc ctttaagacc gggcggtatt caaccaccgc cggttaagcg
    54661 acgcaataaa agatggcggt cattttacag aaggcagcgt aacgagaatg atttaacgca
    54721 taaaaaagca gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat caactaactg
    54781 atttcgattt atgccacaaa tttcccgcta cagtgatgaa caggttgaac aattgctcgc
    54841 agagctgctc aacgtactgg aaaaacataa ggctccgacc gacctttccc tgatggtgtt
    54901 aggcaacatg gtcactaacc ttatcaatac cagcattgcc ccggcccaac gccaggcaat
    54961 tgccaactct tttgcccgcg ccttacagtc ctctatcaac gaagacaaag cgcactaagg
    55021 gaaacagata acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa gtctcccaga
    55081 tggtcagttg ggggcactgg tttgcactgt tcaatattct gctttcgctc gtcattggca
    55141 gccgttacct gtttatcgcc gactggccga caacgcttgc tggtcgcatt tattcctacg
    55201 taagcattat cggccatttc agcttcctgg tgttcgccac ctacttgctg atcctcttcc
    55261 cgctgacctt tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc attctggcaa
    55321 cggcgggaat gacgctatta ctgatcgata gcgaagtctt tactcgtttc catctccatc
    55381 ttaatcccat cgtctggcaa ctggttatca acccagacga aaatgagatg gcgcgcgact
    55441 ggcagctgat gttcatcagc gtgccggtta ttttattgct tgaactggtg tttgcgacgt
    55501 ggagctggca aaagctgcgc agcctgacgc gtcgtcggcg cttcgcgcgc ccgctggccg
    55561 cattcttatt tatcgccttt atcgcctcgc atgtggtgta tatctgggcc gatgccaact
    55621 tctatcgccc gatcaccatg cagcgcgcta acctgccgct ttcgtacccg atgacggcgc
    55681 gacgttttct tgagaagcat ggtctgcttg atgcgcagga gtatcaacgc cgtcttattg
    55741 agcaaggtaa tccagacgcc gtttccgttc agtatccgtt aagcgaactg cgctatcgcg
    55801 atatgggcac cgggcagaat gtgctgttga ttactgtcga tggcctgaac tactcacgct
    55861 tcgagaagca gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg ttcacgcgcc
    55921 atatgagctc cggcaacact acagacaacg gcatctttgg cctgttctat ggcatctcgc
    55981 cgagctatat ggacggcatt ctgtcgaccc gtacccctgc ggcgttaatt actgcgctta
    56041 atcagcaagg ctatcagctg ggattattct catcagatgg ctttaccagc ccgctgtatc
    56101 gccaggcatt gttgtcagat ttctcgatgc cgagcgtacg cacccaatcc gacgagcaga
    56161 ccgccacgca gtggatcaac tggctgggcc gctacgcaca agaagataac cgctggttct
    56221 cgtgggtctc tttcaatggc actaacattg acgacagcaa tcagcaggca tttgcacgga
    56281 aatatagccg ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc aatgcactgc
    56341 gtgattctgg caaactggac aatacggtgg tgattatcac tgccggtcgg ggtattccgc
    56401 tgagcgaaga cgaagaaacc tttgactggt cccacggtca tctgcaggtg ccattagtga
    56461 ttcactggcc aggcacgccg gcgcagcgta ttaatgctct gactgatcat accgatctga
    56521 tgacgacgct gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa tattcgcaag
    56581 gtcaggattt gttcaaccct caacgccgtc attactgggt tactgcagcg gataacgata
    56641 cgctggcaat taccaccccg aaaaagacgc tggtgctgaa caataacggt aaataccgca
    56701 cttacaactt acgtggtgaa agagtgaaag atgaaaaacc acagttaagt ttgttattgc
    56761 aagtgctgac agacgagaag cgttttatcg ctaactgatt aattataaat cagttagcaa
    56821 aatatcttac ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga gtcggcacgt
    56881 agcgcagcct ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca aatcctctcg
    56941 tgccgaccaa atcgcccacc ggatatcacc ggaaagcacc ggataagaca gggataaccc
    57001 gctaatcact tgattggcgg gtttttttat gttttttagt caccgaacat caccggattt
    57061 ataagctatc agccggattt tttagttata cgtttagtta tacgggctgg cgtataacta
    57121 aagccgtata actaaaaccc tgttttttcg tgtggaatca tagacatatg gcgagaaaaa
    57181 cacacccatt aaccacagtg cagatcaaag cagccagacc agcgcaaaag gagtacaccc
    57241 tgcaggacgg cggagggctt tttctcctgg tcaaaccgtc tggatcaaaa ctctggagat
    57301 tttcctacta ccgaccatcg gacaaaaaaa gaatattgct gagttttgga tcgcttgaag
    57361 atgtttccct ggctgatgcc agaaaacgcc gtagcgagta caggacgtta atcagtgccg
    57421 gaactgaccc gcaggaccac gagaggcaaa aaagagagac agaggcccga agacaaggga
    57481 acacgttcga aaatgtggcg gcggcatggt accaggtgaa aatcagccag aatctggccc
    57541 ccaacacgat taaagacatc tggcgttcgc tggataaata tgtattcccg ttcatcggca
    57601 acacgccaat agataccctc accgcccgaa ggttcgttga agtgcttacc cccatcaagg
    57661 agcgcggcaa cctggaaaca ctcaagcggg ttttacagcg cgttaatgag gtaatggatt
    57721 acgccgccaa cagtgggctg attgatgcca atccggctat gaatgtgcgt aaggcgtttc
    57781 cctcacctgt aaaaaaacat atgccaacaa tccgccccga acagctgccc gagcttatgc
    57841 aggctttatc agtatcggca acagaacggc agaccagatt actgattgaa tggcagttac
    57901 tgaccgtaac ccgtcccgcc gaagcgtcat caacgcggtg ggatgaaatc aacctggacg
    57961 cgaagcaatg gacgatacct gccggacgca tgaagatgcg cagggatcac gttatcccgc
    58021 tttccggtca ggctatggcg gtgctggagg ccatgaaacc aatcagccac caccgcaatt
    58081 acgttttccc aagtctgaaa gacccacagc agccgatgaa cagccagaca gctaacgcag
    58141 cattgcggcg tatgggattc gctggcgtgc tggtgtctca tggattacgc gccatattca
    58201 gcacagcagc gaacgaggaa ggattcgagc cggacgtaat agaggcggca cttgcccacg
    58261 tcgacaccaa cgaagttaga cgggcataca accggagcaa ctacatagaa aaacgcatcg
    58321 tgctgatgcg ctggtggggc gaatttgtcg aggctgcggc gacgggcgta accctcgcca
    58381 gtggtaaaag gggtatccga gccgtgtagc tgtacagaaa accagtaaaa actacgaaaa
    58441 ccatgtaaaa ccgtcgtata attgcatcaa atttaacgac aaggccgtga aacatgaaac
    58501 cgttaagatg caaaaaaatt tcagatgcaa ttgcgacggg ctgcaactgg ccctgatggt
    58561 tcagcatgaa ttttggtcaa cctacgatcc ggaggacaga acgacggccc catcaaaaaa
    58621 acaggtagta gatttcctgg tatcccgtgg ggcatccagg aatatggctg aaagtatcga
    58681 cagggtagtt cgtccggcaa ccatgaagac cggaggcagg ccaaaaaaat ggcggtaaca
    58741 atcctcaaag cggcagaaat gccgcttttt ttataaatcc ctttcaaatc accaacataa
    58801 aaaacgattc taaccgttta aaaacggtgg gaactgtttt tacccatatc cgatgattta
    58861 ccgtatttgt caccggaata caccggattc acaaggtaaa tcacgatgga agcaatcaaa
    58921 aaagtcatct ttcgccagga agtaaaaaaa cttatccaca tcaaggcaga cagcacgctt
    58981 caaagcatga tcaacgccgg agaatttccg cagggttttc gcgttggttt acgccgtcgc
    59041 ggatggtatg aggaagacgt aatcgcatgg atgaaagaac gtgagcagga agcacgcgga
    59101 acggctgcgt aatggtgtga ggcataacac gatgaacata acaaaaagcg ccccgttgcc
    59161 ggaacgccct tgtgaacaat taacctgctg cgccttatgt gatcccaaac ataagcacgg
    59221 gaatgatagc cgctatcagg ctggtgggca atgtaattac cctttgggtt ctatgccgcg
    59281 ttgctgtaat tctttgcgga ttattcgctt tatccaggca gcaagagaat catcgccgtc
    59341 atgttgttgt gccttttcca tcttttcccg taagtcagga tctaaacgga actggaacgg
    59401 aggattacca cgcctttcgt ttttgtgtat tgacacgtta attacacctc atgtaatgta
    59461 tttatgtgta ttgacacatt acacacaatc aacacaaaaa gcaaagcccg ctggcgctat
    59521 gaacaccaac gggcttctaa ccaaaccatt aattgaggta acgattatgg ctataaaaga
    59581 gcatagctta ctctttatac acacacaaac gccccggaaa cagaataatc cgtttcatag
    59641 ttcggttttg tgcactcatc ctggcggtcg gtttttgccg gatttaagcc gccgtgggta
    59701 tttatcgaca aaatcttcaa gggcaaattt ttctggtggc actaagcgcg gcgctggtgg
    59761 tggtattttt gttctggtta gttcttcctc aagtctggcg caggcttctg actgcctttg
    59821 ccggatgatt tcatcatctt gcgtttgcgt gtcttgtatt ggtgatagtg tgttgttggt
    59881 catgatactg ccctgtaaag caatgcgccg tagtacctca caccacggcg ctgatagtga
    59941 ttattctgat tctttggcct tgcggcgctg gcggcgtttg atctcgcctc gcatggctgt
    60001 aacgataaat tgcgcagtgc tctcgcctgt ttcttttact tgctccatag cctcaacaac
    60061 ttcatgcggg gcgcgagcct gtaatttctg tgatttgtta ttgatatggt ctctttccat
    60121 ttctggttcc ctttgtgatt actggaatcc agtatacacg aaaaagacca aaacaaaacg
    60181 cttgaagtgg attccactta gtgattatac tggaatccag ttgagtgttt atcactcaat
    60241 gcaaacgagg ctgaaaagga ttgccgtcct ttcgcagcct ctgaccacca acgatagcaa
    60301 cagtatcgag gtagctatga gaaatcatat cacacacccg caagggcggg acccgcacaa
    60361 cctgaataaa tacatctggc gttttatcgc cctgagcacg gctaagccgc gcgtaatcca
    60421 catcgtggcc accagcgaac aggaagcccg tcggcaatca ccggctggct gcgtgatggt
    60481 attcactgcc cgtattcgtc agggggtgga cttatgagcc aggaaatcac actacaacag
    60541 gcagcagaac gcgcccacca aatcgaagtt atttgcgcac tggcagagga ttaccctggc
    60601 atgatgaccg acagcgaatc aggggcaatc atcggcttac ttaaacgcct tagcggtgag
    60661 gtctgcgtat tcctgagcga tgagcaggaa agaagaacgc ttatttctaa cgaaaaaaaa
    60721 tgcggagggg tacaccatgt gcaataacat ccgtccggac gcaaccgccg ccgcgctcac
    60781 tacgctgatg ctcgcgctta ttgatatttc tgttattgct gacagggcgc ataagcacgc
    60841 caccagtgaa acagaatatg caggggcttt cgttcctcat tcgctggcgg ttatgcaact
    60901 tagtgctgat atggcgctga atgaggccaa agccatcctg attgctgatt gtgaaaatgg
    60961 gggggggtta tgcgtgatga tcgttttaat tccctgaaac aggaattttc cggcgttcct
    61021 gatgatgcgg ctgatgcgct ttcgtcaatg ccagaactta ttagagcggc ttttttctta
    61081 ctttccacga gagaatataa atcaacgggg cttgatgtac tgaatatcgc cgccgattat
    61141 gcggaatatg tggcagaggc gcgttacaga agaaaatttc ctgaggatgt aagccatgcg
    61201 tgatatttac cacgaaacaa cagatcgcgt atttcttgca ctttctcaca gtgaaaatat
    61261 gatggaaata ttgcgcatat ggcttgaaac acttggcgac aatgaacgcg acaaacaaaa
    61321 atcaagaatt gccacggcat taataacgct tcttgagcct gtaataaatg agttgcagga
    61381 aatagagaca ttgcacgaca gatataacga acagcacacc ggagaataaa aataatgaaa
    61441 cttaaatatt ctggcttaac tgccagtggc aacactcacc ctaaatttac gcgcggtgat
    61501 atttaccgcg accagtacgg cggtacggta atgattaagg gcgtggcggg acggtgcgta
    61561 acttaccgcc gtgaaggtta cgaatatgat tgcgtgatgc ctgtttatca gttccggcgt
    61621 gatttttctc tggtacagac cgcgccgcat aacgtgccca ccagcaacgc cagggcacgg
    61681 gcaaacatcc agaagctgaa aacaatgatt aacggattca ggggtaaaaa atgaaactgg
    61741 caccgaacgt aaaacgatta ccgaaagata aatacaccga tgcgattatt tttgcgggta
    61801 ttgatgctca ttcattcgca gagcattaca tcattgcaca ggccaaaaaa gcaggcgatc
    61861 cagtccctcc cgtttatctg gggcgttatc agttaagcga actggataac ctccagattg
    61921 ttgatgatgg gcgatacagg gcgacggtga tacgcgccgg aaatattgaa gagccgcagc
    61981 tgttaaccat cgccacgaaa ctggcgagcg ccggagtcca ggaggcgcgg ctactttctg
    62041 aaaatttcga attgctggag gaatggagcg accagcttcc acggcttagg gaggcatggg
    62101 aacgcgggga aagcctggtc atgaaaaaaa tcccccagcg aaaaaccaag ctaccaatga
    62161 gcgttggatc taccggatac gacacacagc ttgattacgt ggtaaagggg attattccgg
    62221 catcgtcgct ttgcagcata tacggggcga gcggttccta taaatcattc cttgccggat
    62281 cgtgggcgtg tcacgtttcc acgggccgcc agtggggagg ccgcagggtg gcgcatggcg
    62341 ctgttctcta tgtggttggt gaaggcggta taggcgttcc gcgtcgtgta aaagcctggg
    62401 aggttgtgca cgatgagcag gtgaaaaatc tgtatctggt aaaccgtccc atctttccgg
    62461 ctgccccgct tgatgttgag gaaatggtta tcgctgcccg tcaggtggaa cgggaaacgg
    62521 gtaaaccggt tcgaatgatt attctggata cgctggcgcg ctgctttggc ggcaatgatg
    62581 aaaatgattc ccgtgatatg ggggcgttta tccgtgggtg tgacgaactg aaacggcgca
    62641 caggggcaac ggtgctggtg gttcaccatt ccggcaagga tgaaacaaag ggggcgcgtg
    62701 gttccagtgc atttcgtgct tcgctggatg ctgaataccg gatacgcagg gaggacgcag
    62761 gcagcgaagc gctggttatc tcatgtacca aaatgaagga cgcggaggaa ctcaaagaag
    62821 cagcatatga cttacgcgtg gtggagcttt ttaccgacgc tgacggggag ttaatcacgt
    62881 cgctggtggt ggtggataag ccgcgccctc ccgttgaact ggagcgcatc gaggaggcag
    62941 ggaacaagac ggagaatcac gccgcgctat ggcagtgcat cagaacacga acagcacacg
    63001 gggaggcgtg cactatcgcc cttctccgcg acgatatgaa aaagctgggg tatgagatga
    63061 agcacttccg gcgctggctg tacaagctgg aaaatgatgg cgtaatcgtt attgacggtg
    63121 atgacgtgcg cccgctgtaa aaagtgggga gtaaaagtgg ggggagtggg gaatttaaca
    63181 aaattgaaac ataattaccc actttcccac ctgtatatac ccaaaaaagt ggggagtaaa
    63241 aaacatattg aaaaacataa cgttagaatc acaaaaaaaa gaggtgggga gacggtgggt
    63301 aatttcaaaa agtggggagc aaaagtgggg agtagtgggg aatgaacaga aaaaccagag
    63361 ataagacagc gccaaaatat aaagcgttag acatgacaga gctttcctta aaggtggcaa
    63421 tcagaacgat agaccgccac gcgggggaag gatacgcgaa ggagcatccc gaactgataa
    63481 gcgcattcat gaccacagcg gcggcaaact ttgccacgct gacagaacgg gagattgccg
    63541 aagcggaaca ggtaacaacc atcaacgtta aaagcgctgg agaataaccg aagaagtgag
    63601 cacatagccg gagcaatccg gctttttttc gatctcatat atcttcaaag atggttaatt
    63661 aatgataatc tatcgaaaat actggttatg caatgataag tatcaggaga aaacaaagtg
    63721 gaagagataa agcaaagcgc taccgtgatg attgatcatg aaatccttaa cgagcttaaa
    63781 aagttgcagt tcgatattgt gcgccggatg aatgaatcag gtatctacgg cagggcggca
    63841 acaccaacaa tagggcatct tgcgcgcgta ctgttacgcg aacgcctggg cattgcaaca
    63901 agtgaggcgt aaacaatggc tatcacagaa cgcgaggcaa gcatcattaa agccattggc
    63961 gaggaaacaa gagacaccat agcgccattg atggaaaaga tcgcagagct tgagacacga
    64021 ataaacaagc tgagtgatga agttgaaggc ctgatctcgt tactcggtga gcaatgacag
    64081 ggggggagtg ttccggccgg aaagtcgatc gccctggaca ccgcccccag tctcattcgt
    64141 agaaaatttt gcttttcaaa aaagttacac gaaaagttac cccccctcaa aacgaggttt
    64201 ttgcatggca ttaacgacca aaaaaaagcg gtttgctgat gcgttatcaa ggggcgcaaa
    64261 tcccacggaa gccactatac atgcgggata cagcgaaaag accgcaaggg tcaaaggctg
    64321 gcagctatcc aacgatcctg aggtgaaaaa gtatcttaag gctgtaactc ctgatgtaac
    64381 ttttcaggtt acaaaaccga aaaaagttac aaagaaagtt accgaaaaag ttacaccgga
    64441 acgggcatca attaacacca ttgagatgat ggataacggc ctacccgacc ccatcaaagc
    64501 aatgggcagg atactgattg acaacatgga cacagatcca aaactggctt tagatgccgc
    64561 gtttaagctg gcccagttca cccaccgcaa gatcggcagc attgggaaaa aagaggttaa
    64621 aaaactcaat gccgctagtg tctactcaaa tcggttcccc atccctaagc caccaacaga
    64681 cttaaaacac tgaggtagcg aactatgagc gaatacatca cagaccctat tgatgtactc
    64741 acggaaatca tgaacgacaa gcagcagtcg accgaaatac ggttacaggc cgccgctgtt
    64801 ctgatgccat attttcacga aacactaaac ccgtgcttcc aggaggagga agaagatgat
    64861 ctgtaaaaac atgaaaggcg tgtcagcagt tcgcgccgcc agcgtgctgg catcgtgtaa
    64921 atttagccat gctgacgcta tagagatgac gaaaagaaat gatccggcag tatcgttatt
    64981 tcttgaaaaa tccgtagtag caggaatgaa cacggataac atgccgtcgc tggtggcaga
    65041 gccgatagta agagagcttg gcgagtatat tttttcgcag tctgtaccag gcaagctaat
    65101 cagcagcgcg ctaaatcttc cttttggcgc aaaattgcag tcaatatcag gcaagggcag
    65161 taaatggctt aaagaagccg aagaaatccc tgtcaaagag ggagaagtaa acgaaagtgc
    65221 gctaaaactg tgcaaacttg gcgctattgt gatgctgaat aacgagctaa tcaaatttag
    65281 cacccctggt acaaattcag cgatcagaga cttgatcaca tcggaatcgg tgaaagaaat
    65341 agataaaaaa tttctttcca gtgaccagga ggtggctggc gtatcccctg agggcgtgct
    65401 taatggtgct gatgaggccg aaaacttcat agagcttttc aaaaagcaca tagcgaacgg
    65461 taacacgctt tccacgtcgt cgctaatctt gcccgtagaa aacgccttac agcttactga
    65521 tgctgagttc tggcagatag agcttttgag aataccggta attgtgagcg aacaggcgga
    65581 tcgcatgatg ctgatagacg ctcaaaagct gattattaac gttgaagcca cggttataac
    65641 cccaactgat gaggcggtaa taaaaaccga atcaggggcg gttagccttt tccagaatga
    65701 cgctacagcg ttcagggcaa tcacctattg cggatggcaa aaactcgata aggccgtcac
    65761 cgttctggcg aaaagctgat ttttccggca tcaatcccga tatggtgatc cccatatcgg
    65821 gataattacg aaattttcgc agtttaatta atggtcagat taatcgctaa cccgctgata
    65881 ttttcggaaa cctcaatttg aggaagtcgg aagcgtcacc cttcaaattc tgaatggtca
    65941 taatgaccac gcagaccatc aaggcaacca taacgaacat cgttacggtt ggagctttta
    66001 ccacaggaaa actcgcattt aaattgtac
//
LOCUS       DAZFRN010000004        65557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFRN010000004 DAZFRN010000000
VERSION     DAZFRN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.004"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..446)
                     /gene="folK"
                     /locus_tag="ACHKUB_000223"
     CDS             complement(<1..446)
                     /gene="folK"
                     /locus_tag="ACHKUB_000223"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO6653306.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLSHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVL"
     gene            complement(443..1840)
                     /gene="pcnB"
                     /locus_tag="ACHKUB_000224"
     CDS             complement(443..1840)
                     /gene="pcnB"
                     /locus_tag="ACHKUB_000224"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO6653307.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1900..2796)
                     /gene="gluQRS"
                     /locus_tag="ACHKUB_000225"
     CDS             complement(1900..2796)
                     /gene="gluQRS"
                     /locus_tag="ACHKUB_000225"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO6653308.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2863..3318)
                     /gene="dksA"
                     /locus_tag="ACHKUB_000226"
     CDS             complement(2863..3318)
                     /gene="dksA"
                     /locus_tag="ACHKUB_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO6653309.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3496..4200)
                     /gene="sfsA"
                     /locus_tag="ACHKUB_000227"
     CDS             complement(3496..4200)
                     /gene="sfsA"
                     /locus_tag="ACHKUB_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO6653310.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4215..4745)
                     /gene="thpR"
                     /locus_tag="ACHKUB_000228"
     CDS             complement(4215..4745)
                     /gene="thpR"
                     /locus_tag="ACHKUB_000228"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO6653311.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDAREAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4819..7248
                     /gene="hrpB"
                     /locus_tag="ACHKUB_000229"
     CDS             4819..7248
                     /gene="hrpB"
                     /locus_tag="ACHKUB_000229"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO6653312.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGRASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSML
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDNESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7444..9987
                     /gene="mrcB"
                     /locus_tag="ACHKUB_000230"
     CDS             7444..9987
                     /gene="mrcB"
                     /locus_tag="ACHKUB_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO6653313.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYDDYE
                     DEEPMPRKGKGKGKGRKPRSKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKV
                     WQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRR
                     PFDFPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLF
                     VPRSGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLV
                     KNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLAS
                     LYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQE
                     LYDMLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQ
                     DAAEKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQAR
                     RSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRV
                     MLVDALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEV
                     AQAFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQ
                     RGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGAS
                     GAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQ
                     QSEMQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10207..12450
                     /gene="fhuA"
                     /locus_tag="ACHKUB_000231"
     CDS             10207..12450
                     /gene="fhuA"
                     /locus_tag="ACHKUB_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO6653314.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFATEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12501..13298
                     /gene="fhuC"
                     /locus_tag="ACHKUB_000232"
     CDS             12501..13298
                     /gene="fhuC"
                     /locus_tag="ACHKUB_000232"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO6653315.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13298..14188
                     /gene="fhuD"
                     /locus_tag="ACHKUB_000233"
     CDS             13298..14188
                     /gene="fhuD"
                     /locus_tag="ACHKUB_000233"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO6653316.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FILSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14185..16167
                     /gene="fhuB"
                     /locus_tag="ACHKUB_000234"
     CDS             14185..16167
                     /gene="fhuB"
                     /locus_tag="ACHKUB_000234"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO6653317.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFVLAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16202..17482)
                     /gene="hemL"
                     /locus_tag="ACHKUB_000235"
     CDS             complement(16202..17482)
                     /gene="hemL"
                     /locus_tag="ACHKUB_000235"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO6653318.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17648..17695
                     /gene="yadX"
                     /locus_tag="ACHKUB_000236"
     CDS             17648..17695
                     /gene="yadX"
                     /locus_tag="ACHKUB_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO6653319.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17707..19128
                     /gene="clcA"
                     /locus_tag="ACHKUB_000237"
     CDS             17707..19128
                     /gene="clcA"
                     /locus_tag="ACHKUB_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO6653320.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19152..19217
                     /gene="yadW"
                     /locus_tag="ACHKUB_000238"
     CDS             19152..19217
                     /gene="yadW"
                     /locus_tag="ACHKUB_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO6653321.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19210..19554
                     /gene="erpA"
                     /locus_tag="ACHKUB_000239"
     CDS             19210..19554
                     /gene="erpA"
                     /locus_tag="ACHKUB_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO6653322.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19601..20224)
                     /gene="yadS"
                     /locus_tag="ACHKUB_000240"
     CDS             complement(19601..20224)
                     /gene="yadS"
                     /locus_tag="ACHKUB_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO6653323.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20262..21062)
                     /gene="btuF"
                     /locus_tag="ACHKUB_000241"
     CDS             complement(20262..21062)
                     /gene="btuF"
                     /locus_tag="ACHKUB_000241"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO6653324.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21055..21753)
                     /gene="mtnN"
                     /locus_tag="ACHKUB_000242"
     CDS             complement(21055..21753)
                     /gene="mtnN"
                     /locus_tag="ACHKUB_000242"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO6653325.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21837..23354
                     /gene="dgt"
                     /locus_tag="ACHKUB_000243"
     CDS             21837..23354
                     /gene="dgt"
                     /locus_tag="ACHKUB_000243"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO6653326.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23484..24908
                     /gene="degP"
                     /locus_tag="ACHKUB_000244"
     CDS             23484..24908
                     /gene="degP"
                     /locus_tag="ACHKUB_000244"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO6653327.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25063..26220
                     /gene="cdaR"
                     /locus_tag="ACHKUB_000245"
     CDS             25063..26220
                     /gene="cdaR"
                     /locus_tag="ACHKUB_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO6653328.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26309..26695)
                     /gene="yaeH"
                     /locus_tag="ACHKUB_000246"
     CDS             complement(26309..26695)
                     /gene="yaeH"
                     /locus_tag="ACHKUB_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO6653329.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27010..27834)
                     /gene="dapD"
                     /locus_tag="ACHKUB_000247"
     CDS             complement(27010..27834)
                     /gene="dapD"
                     /locus_tag="ACHKUB_000247"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO6653330.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27865..30537)
                     /gene="glnD"
                     /locus_tag="ACHKUB_000248"
     CDS             complement(27865..30537)
                     /gene="glnD"
                     /locus_tag="ACHKUB_000248"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO6653331.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30599..31393)
                     /gene="map"
                     /locus_tag="ACHKUB_000249"
     CDS             complement(30599..31393)
                     /gene="map"
                     /locus_tag="ACHKUB_000249"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO6653332.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31761..32486
                     /gene="rpsB"
                     /locus_tag="ACHKUB_000250"
     CDS             31761..32486
                     /gene="rpsB"
                     /locus_tag="ACHKUB_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO6653333.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32744..33595
                     /gene="tsf"
                     /locus_tag="ACHKUB_000251"
     CDS             32744..33595
                     /gene="tsf"
                     /locus_tag="ACHKUB_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO6653334.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDVNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVTASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33742..34467
                     /gene="pyrH"
                     /locus_tag="ACHKUB_000252"
     CDS             33742..34467
                     /gene="pyrH"
                     /locus_tag="ACHKUB_000252"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO6653335.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34759..35316
                     /gene="frr"
                     /locus_tag="ACHKUB_000253"
     CDS             34759..35316
                     /gene="frr"
                     /locus_tag="ACHKUB_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO6653336.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35408..36604
                     /gene="ispC"
                     /locus_tag="ACHKUB_000254"
     CDS             35408..36604
                     /gene="ispC"
                     /locus_tag="ACHKUB_000254"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO6653337.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36793..37551
                     /gene="ispU"
                     /locus_tag="ACHKUB_000255"
                     /gene_synonym="uppS"
     CDS             36793..37551
                     /gene="ispU"
                     /locus_tag="ACHKUB_000255"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO6653338.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37564..38421
                     /gene="cdsA"
                     /locus_tag="ACHKUB_000256"
     CDS             37564..38421
                     /gene="cdsA"
                     /locus_tag="ACHKUB_000256"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6653339.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38433..39785
                     /gene="rseP"
                     /locus_tag="ACHKUB_000257"
     CDS             38433..39785
                     /gene="rseP"
                     /locus_tag="ACHKUB_000257"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO6653340.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVDQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39815..42247
                     /gene="bamA"
                     /locus_tag="ACHKUB_000258"
     CDS             39815..42247
                     /gene="bamA"
                     /locus_tag="ACHKUB_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO6653341.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42369..42854
                     /gene="skp"
                     /locus_tag="ACHKUB_000259"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42369..42854
                     /gene="skp"
                     /locus_tag="ACHKUB_000259"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO6653342.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42858..43883
                     /gene="lpxD"
                     /locus_tag="ACHKUB_000260"
     CDS             42858..43883
                     /gene="lpxD"
                     /locus_tag="ACHKUB_000260"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO6653343.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43988..44443
                     /gene="fabZ"
                     /locus_tag="ACHKUB_000261"
     CDS             43988..44443
                     /gene="fabZ"
                     /locus_tag="ACHKUB_000261"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO6653344.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44447..45235
                     /gene="lpxA"
                     /locus_tag="ACHKUB_000262"
     CDS             44447..45235
                     /gene="lpxA"
                     /locus_tag="ACHKUB_000262"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO6653345.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45235..46383
                     /gene="lpxB"
                     /locus_tag="ACHKUB_000263"
     CDS             45235..46383
                     /gene="lpxB"
                     /locus_tag="ACHKUB_000263"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO6653346.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46380..46976
                     /gene="rnhB"
                     /locus_tag="ACHKUB_000264"
     CDS             46380..46976
                     /gene="rnhB"
                     /locus_tag="ACHKUB_000264"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO6653347.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47013..50495
                     /gene="dnaE"
                     /locus_tag="ACHKUB_000265"
     CDS             47013..50495
                     /gene="dnaE"
                     /locus_tag="ACHKUB_000265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO6653348.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50508..51467
                     /gene="accA"
                     /locus_tag="ACHKUB_000266"
     CDS             50508..51467
                     /gene="accA"
                     /locus_tag="ACHKUB_000266"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO6653349.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51565..53706
                     /gene="ldcC"
                     /locus_tag="ACHKUB_000267"
     CDS             51565..53706
                     /gene="ldcC"
                     /locus_tag="ACHKUB_000267"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO6653350.1"
                     /translation="MNIIAIMGLHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53763..54152
                     /gene="yaeR"
                     /locus_tag="ACHKUB_000268"
     CDS             53763..54152
                     /gene="yaeR"
                     /locus_tag="ACHKUB_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6653351.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALKGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54217..55530
                     /gene="tilS"
                     /locus_tag="ACHKUB_000269"
     CDS             54217..55530
                     /gene="tilS"
                     /locus_tag="ACHKUB_000269"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO6653352.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMVAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETILPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(55564..55824)
                     /gene="rof"
                     /locus_tag="ACHKUB_000270"
     CDS             complement(55564..55824)
                     /gene="rof"
                     /locus_tag="ACHKUB_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO6653353.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55811..56011)
                     /gene="yaeP"
                     /locus_tag="ACHKUB_000271"
     CDS             complement(55811..56011)
                     /gene="yaeP"
                     /locus_tag="ACHKUB_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO6653354.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56177..56722
                     /gene="yaeQ"
                     /locus_tag="ACHKUB_000272"
     CDS             56177..56722
                     /gene="yaeQ"
                     /locus_tag="ACHKUB_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO6653355.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            56719..57141
                     /gene="arfB"
                     /locus_tag="ACHKUB_000273"
     CDS             56719..57141
                     /gene="arfB"
                     /locus_tag="ACHKUB_000273"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO6653356.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57155..57865
                     /gene="nlpE"
                     /locus_tag="ACHKUB_000274"
                     /gene_synonym="cutF"
     CDS             57155..57865
                     /gene="nlpE"
                     /locus_tag="ACHKUB_000274"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO6653357.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(58021..58845)
                     /gene="yaeF"
                     /locus_tag="ACHKUB_000275"
     CDS             complement(58021..58845)
                     /gene="yaeF"
                     /locus_tag="ACHKUB_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO6653358.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(58898..60616)
                     /gene="proS"
                     /locus_tag="ACHKUB_000276"
     CDS             complement(58898..60616)
                     /gene="proS"
                     /locus_tag="ACHKUB_000276"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO6653359.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60727..61434)
                     /gene="tsaA"
                     /locus_tag="ACHKUB_000277"
     CDS             complement(60727..61434)
                     /gene="tsaA"
                     /locus_tag="ACHKUB_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO6653360.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVKKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61431..61835)
                     /gene="rcsF"
                     /locus_tag="ACHKUB_000278"
     CDS             complement(61431..61835)
                     /gene="rcsF"
                     /locus_tag="ACHKUB_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO6653361.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61953..62768)
                     /gene="metQ"
                     /locus_tag="ACHKUB_000279"
     CDS             complement(61953..62768)
                     /gene="metQ"
                     /locus_tag="ACHKUB_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO6653362.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62808..63461)
                     /gene="metI"
                     /locus_tag="ACHKUB_000280"
     CDS             complement(62808..63461)
                     /gene="metI"
                     /locus_tag="ACHKUB_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO6653363.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63454..64485)
                     /gene="metN"
                     /locus_tag="ACHKUB_000281"
     CDS             complement(63454..64485)
                     /gene="metN"
                     /locus_tag="ACHKUB_000281"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO6653364.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64673..65245
                     /gene="gmhB"
                     /locus_tag="ACHKUB_000282"
     CDS             64673..65245
                     /gene="gmhB"
                     /locus_tag="ACHKUB_000282"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO6653365.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15481 a        16303 c        18070 g        15703 t
ORIGIN      
        1 tgtaagactt cacgcaacgt ctccccatca ggaaacgcca gctccggtgc aatctcaaac
       61 agcggccaca gcataaatcc acgattcttc atatcgtagt gcggaacggt caggcgttcg
      121 gtatttatca cttcattacc aaacagcatg atatcgagat ccagcgtgcg tggtccccag
      181 cgttcagctt tacgaacacg accctgttgc agttctatgc gctgtgtatg gctgagtaac
      241 tcttccgggg cgagcgaagt ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct
      301 tgcggcccca gcggtggagt gcggtaaaac gaagaaacgg caagaatgtg gctttcaggg
      361 atatcgccta atgctttcag ggcagcattg acctgctcca gcggagaggc cagattgctg
      421 cctatggcaa tatacgccac tgtcatgcgg taccctcacg acgcggtgcg cgtttgcgtg
      481 gacgacgagt acggcgacgc ggtgacggct cttcatccaa ctcgttaagc atcccttttt
      541 gatctggtgg tgcggaaacc tggaactcac cccaccattt caccagacgc tgcagttcag
      601 cattacgttc aacttcagct cgcaaggcca acaggtcata agccgcacgg aacttaggat
      661 gctccagcag tttccatgcg cgtttaccct gacgacggga catacgcaac tgcaactgcc
      721 agatatcgcg ggttaacgtc gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt
      781 ccagcacgtc gttcatcgcc agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg
      841 cgatcttctg tgccgtctcc agcagtgggt accagaacat ggcggcaaac aagaacgccg
      901 ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagcacc tgttcaatga
      961 tccgctccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc gggaacagcg
     1021 gctggaacag atgatattca cacaacagct tataggtttc gtaaccgtag cccgcttgta
     1081 gcagtttaag cgattcttca aacaggcgtg ccggtgggat atcgttcagc agggtagcga
     1141 ggcgagggat cggttctgcg gtttccgggc tgatgcgcat acccaatttg gcggcaaaac
     1201 gtaccgcgcg cagcatacgt accggatctt cacggtagcg cgtttccggg ttaccaatca
     1261 gacggataac gccgtccttc agatccttca taccgccaac gtaatcacgg acggtaaaat
     1321 ccgctacgct gtaatagagg ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga
     1381 tggagccgaa aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc
     1441 ggtcgctgac gttaccttcg tggtgtccac ggaaggttgc aacttcgata atctccgggc
     1501 caaacatcac atgagccaga cggaaacggc gacccaccag gcggcagtta cggaacagtt
     1561 tgcgcacctg ctcaggcgtg gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa
     1621 gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct ttgttgagcc
     1681 tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca tgctgctcac
     1741 gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc
     1801 ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc ggtagtgtca
     1861 aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg catttgagaa
     1921 tgttgaattt acaattgccg actcgggcac ggcggtaagc cgccagtttt tgacggctga
     1981 ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa
     2041 ttgaagtgcc gcgattagta ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt
     2101 ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg
     2161 cactttccag ccaaaaagct ggtacagcga gatttgcctt actgttggtt caatcagatc
     2221 agccccacgc actatttctg taacgccctg gaaatgatca tcaaccacaa cagccaggtt
     2281 gtaggcgaac aacccatcac ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc
     2341 ggcgtgaata atgccgcgca gctggtcagt aaattgcgtg accggatgct gctggcggat
     2401 acgcactgcg gcattgtctg gtccatgatg caacacccgg caatgaccgt cgtaaatacc
     2461 gccaatgctt tgaatacgcg cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg
     2521 taaccaggcg agtgcttcac gataggcgtg gtgacgttgc gattgccaga gaacatcgcc
     2581 gtcccagtgc agaccgtaat gttccagctg gcgcaggata gtttctgcgg caccgggaac
     2641 ttcacgaggc gggtcgatat cttctatgcg taccagccag cgaccttgcc gggcgcgagc
     2701 ctgcaaatag ctgccgagcg cggcgatcag agagccaaaa tgaagctcgc cggaaggaga
     2761 gggggcgaag cggccaatat actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg
     2821 gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag ccatctgttt
     2881 ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc
     2941 cagacggcga ataccaattt caacaccgca ggattcgcag tagccgaaat cttcgtcttc
     3001 cacttttttc agcgtcttct cgatcttttt gatcagctta cgctcgcgat cgcggttacg
     3061 cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg ggaagttggc
     3121 tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt gattacgcca
     3181 tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact cttcgcccgg
     3241 cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg atgttttacg
     3301 gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc catgttcggg
     3361 ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta tctaaacgta
     3421 acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac
     3481 cagttactta ctttactaca atgtaaccgg cagtgatttt ttaagagcca tgccttcagc
     3541 agaaatttcc gctttgtaag ccagaatttc tacccccctc tgttgagctt ctgacaatag
     3601 ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag
     3661 cacggcgaaa aagataaccg cacgctggcc ttcagccgct acgctcatca actcccgaag
     3721 gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa
     3781 cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca acataaagtc
     3841 aatacggctg cgttctgcgc cgtattttac ttcgcttttc agcgagctat agcctgacag
     3901 ttctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc aaagcgtgtt
     3961 gacgcaaata aatgcgccgc tctggctttg agttaattcc caggtgtgtg ggtatttccg
     4021 tttggtgttg tctgaagtcg aataccagac ggtatcgcca ggcgttgcac aaccggtcat
     4081 tgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc
     4141 taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt agagggggag agaattccat
     4201 tgcgacaatc cttgttattg cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt
     4261 ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgcatacga ccagttaaaa
     4321 cctggcggcg ggattgtcac cgcctctctg gcgtcgcgca ataaggtaat atgtggatga
     4381 aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgaacggag catattcgcc
     4441 agctggatta agccgcgtgg cggctgacgc atccctaacc acaccacacg cgaacgcagc
     4501 cattgtccgg cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa
     4561 agagaaagcg ccttctcttt ctcggcgctc acttcgccta aaaatgccag agtcagatgc
     4621 aaattatcgg cggcgaccgg acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg
     4681 ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagacg ttgcggttca
     4741 gacatgtgag gcactcggtt atgaattacc gcgatgctac aatgtggcgc gaagaatgtt
     4801 aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt
     4861 accgctctcg attgtgcgcc gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca
     4921 acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat tatcctgctg
     4981 gagccgcgtc gtctggcggc gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa
     5041 aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc
     5101 cgcctggaag tggttaccga aggcgtgctg acgcgcatga tccagcgtga cccggaactg
     5161 agcggtgttg gactggtgat ccttgatgaa tttcatgagc gcagcttgca ggcggatttg
     5221 gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt
     5281 atgtcggcta ccctggacaa cgaccgcttg cagcaaatgc tgccagaagc gcctgtcgtc
     5341 atctcagaag ggcgctcgtt tccggttgaa cgccgttatt taccgctgcc cgcgcatcag
     5401 cgttttgacg atgccgttgc ggtagccacc gctgaaatgc tgcgtcagga aagcggatca
     5461 ttactgttat ttttacctgg cgtcggagaa attcagcgtg tgcaggaaca actggcttcg
     5521 cgcatcggca gtgatgtatt gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag
     5581 cgaaaagcga tcctcccggc accgcaaggg atgcgcaaag tggtgctggc gaccaatatt
     5641 gctgaaacca gtttaaccat tgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt
     5701 gtggcgcgtt ttgatccgcg cacggggctt acgcgactga ttactcaacg cgttagccag
     5761 gcatccatga cgcagcgtgc cgggcgcgcc gggcgtctgg agccgggtat ctgcctgcat
     5821 ttaatcgcca aggaacaagc agaacgcgct gccgcgcaaa gtgaaccgga aatcttacaa
     5881 agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcagcga tccggcgcag
     5941 atgagctggc tggatcaacc gccaacggtg aatctactgg ccgcgaaacg cctgttacaa
     6001 atgctggggg cgctggaggg tgaacgactt agtgcgcagg ggcaaaaaat ggcagcgctg
     6061 ggtaacgatc cgcgtttagc ggcaatgctg gttagcgcga agaacgacga cgaagctgct
     6121 accgcggcaa aaattgccgc cattctcgaa gagccgccac ggatgggcaa tagtgacctg
     6181 ggcgtggcgt tttcgcgcaa tcaaccagcc tggcagcaac gtagtcagca actgttaaaa
     6241 cgcttaaacg tacgtggcgg tgaggcagac agttcgctta tcgcgccgct acttgccggg
     6301 gcgtttgccg atcgcattgc tcgtcgccgt gggcaagatg gacgctatca actggcaaac
     6361 ggcatgggag cgatgctcga tgccaacgac gcgctaagcc gccacgaatg gttgatcgca
     6421 ccgttattat tgcagggccg cgcctcgccg gatgcgcgga ttttactggc gctaccggtc
     6481 gatattgatg agttagtaca acgctgcccg cagctggtac agcagtctga cactgtggag
     6541 tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tgcaaatcgg tctgctgacg
     6601 gtgaaagtgc agccgctggc gaaaccctcg gaagacgagt tgcatcaggc gatgcttaat
     6661 ggcattcgtg ataaaggttt aagcatgctc aactggacgg cggaagcgga acagctacgc
     6721 ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggcctgc ggttgataat
     6781 gaaagtttgt tggcgacgct ggaaacgtgg ctgctgccac atatgaccgg cgtgcattca
     6841 ctacgcggcc tgaaatcact cgacatttat caggcactac gcggattact tgattgggga
     6901 atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac gggaagccgg
     6961 atcgccattc gttatcatga agataacccg cccgcgctgg cggtgagaat gcaggagatg
     7021 tttggtgagg ccaccaatcc gacgatcgcc caggggcgcg tgccgctggt gctggagttg
     7081 ctttcacctg cccaaaggcc attacaaatc acacgagatt tgagcgcctt ctggaaagga
     7141 gcgtaccgtg aggtgcaaaa agagatgaaa gggcgttatc ccaaacatgt ctggccggac
     7201 gacccggcaa atactgcacc gacgcgacgg acgaaaaagt attcgtaagg ttggttttct
     7261 ccctctccct gtgggagagg gccggggtga gggcatcagc gcgcacgttc accctcaccc
     7321 taaccctctc cctcaaggga gaggggaccg atcgagcaca aattttgaga gatatcttct
     7381 tctgtcttgt aacagaagaa cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa
     7441 agcatggccg ggaatgaccg cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc
     7501 aaacaaaagg taagccgtcg tcgttacgaa gatgacgatg attacgatga ttacgacgat
     7561 tatgatgact atgaggatga agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg
     7621 cgtaagcctc gtagcaaacg cggctggcta tggctactgc taaaactggc tatcgttttt
     7681 gccgtgctga tcgccattta cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat
     7741 ggcaaggtct ggcaactgcc tgcggcagtt tatggccgaa tggtcaatct tgagccagat
     7801 atgaccatca gcaagaacga gatggtgaag ctgctggagg cgactcagta tcgtcaggtg
     7861 tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca acagcattga gatgatccgc
     7921 cgtccgtttg atttcccgga cagtaaagaa gggcaggtgc gtgcgcgtct gacctttgat
     7981 ggtgatcatc tggcgacgat cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt
     8041 cttgatccgc gcctgatcac catgatctct tcgccaaacg gtgagcagcg tctgtttgtg
     8101 ccgcgcagtg gtttccctga tttgctggtg gatactttgc tggcgacaga agaccgccat
     8161 ttctacgagc atgatgggat cagcctctac tcaattgggc gtgcggtgct ggcaaacctg
     8221 accgcaggtc gcacggtgca gggtgcgagt acgctgacgc aacagctggt gaaaaacctg
     8281 ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg aagcttacat ggcgctgatc
     8341 atggacgcgc gttacagcaa agaccgtatt cttgagctgt atatgaacga ggtgtatctc
     8401 ggtcagagcg gcgacaacga aatccgcggc ttcccgctgg cgagcttgta ttactttggt
     8461 cgcccggtag aagagctaag cctcgaccag caggcgctgt tagtcggcat ggtgaaagga
     8521 gcgtccatct acaacccgtg gcgtaaccca aaactggcgc tggagcgacg taatctggtg
     8581 ctgcgtctgc tgcaacagca acagattatt gatcaagaac tctatgacat gttgagtgca
     8641 cgtccgctgg gtgttcagcc acgcggtggg gtgatctctc cacagccagc ctttatgcaa
     8701 ctggtgcgtc aggagctgca ggcgaaatta ggcgataagg taaaagatct ctccggcgtg
     8761 aagatcttca ctacctttga ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa
     8821 ggcattccgg cactgaagaa acagcgtaag ttgagcgatc ttgaaactgc gattgtggtc
     8881 gtcgaccgct ttagtggtga agttcgtgcg atggtcggtg gttctgagcc gcagtttgcg
     8941 ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact
     9001 tatctgactg ccttaagcca gccgaaaatc tatcgtctga atacgtggat tgcggatgcg
     9061 ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac cgcagaatga tgaccgtcgt
     9121 tatagcgaaa gcggcagagt gatgctggtg gatgcgttga cccgttcgat gaacgtgccg
     9181 acggtaaatc tggggatggc gctggggctg cctgcggtta cggagacctg gatcaaactg
     9241 ggcgtaccga aagatcagtt gcatccggtt ccggcgatgc tgctgggggc gttgaactta
     9301 acgccaatcg aagtggcgca ggcgttccag accatcgcca gcggcggtaa ccgtgcaccg
     9361 ctttctgcac tgcgttcggt aattgcggaa gatggcaagg tgctgtatca gagcttcccg
     9421 caggcggaac gcgctgttcc ggcgcaggcg gcgtatctga cactatggac catgcagcag
     9481 gtggtacaac gcggtacggg tcgtcagctt ggggcgaaat acccgaacct gcatctggca
     9541 gggaaaacag ggactaccaa caataacgta gatacctggt ttgcaggcat tgacggcagc
     9601 acggtgacca tcacctgggt aggccgtgat aacaaccagc cgaccaaact gtatggagcc
     9661 agcggggcaa tgtcgattta tcagcgttat ctggctaacc agacgccaac gccgctgaat
     9721 cttgttccgc cagaagatat tgcagatatg ggcgtggact acgacggcaa ctttgtttgc
     9781 agcggtggca tgcgtgtctt gccggtctgg accagcgatc cgcaatcgct gtgccagcag
     9841 agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa
     9901 cagccgcaac agcaacctgc tcagcaagag cagaaagaca gcgacggtgt agccggttgg
     9961 atcaaggata tgtttggtag taattaacat ctaagcgtga aataccggat ggcgagttgc
    10021 catccggtaa aataacatcc catctaagat attaaccctt tcttttcatc tggttgttta
    10081 ttaacccttc aggaacgctc agattgcgta ccgcttgcga acccgccagc gtttcgaata
    10141 ttatcttatc tttataataa tcattctcgt ttacgttatc attcacttta catcagagat
    10201 ataccaatgg cgcgttccaa aactgctcag ccaaaacact cactgcgtaa aatcgcagtt
    10261 gtagtagcca cagcggtaag cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa
    10321 gaagacacta tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg ggggcctgct
    10381 gcaactattg cggcgcgaca gtctgctacc ggcactaaaa ccgatacgcc gattcaaaaa
    10441 gtgccacagt ctatttctgt tgtgaccgcc gaagagatgg cgctgcatca gccgaagtcg
    10501 gtaaaagaag cgcttagcta cacgccgggt gtctctgttg gtacgcgtgg cgcatccaac
    10561 acctatgacc acctgatcat tcgcggcttt gcgacagaag gccaaagcca gaataactat
    10621 ctgaatggcc tgaagttgca gggcaacttc tataacgatg cggtcattga cccgtatatg
    10681 ctggaacgcg ctgaaattat gcgtggcccg gtttccgtgc tttacggtaa aagcagtcct
    10741 ggcggcctgt tgaatatggt cagcaagcgt ccgaccaccg aaccgctgaa agaagttcag
    10801 tttaaagccg gtactgacag cctgttccag actggttttg actttagcga ttcgttggat
    10861 gatgacggtg tttactctta tcgcctgacc ggtcttgcgc gttctgccaa tgcccagcag
    10921 aaagggtcag aagagcagcg ttatgctatt gcaccggcgt tcacctggcg tccggatgat
    10981 aaaaccaatt ttaccttcct ttcttacttc cagaacgagc cggaaaccgg ttattacggc
    11041 tggttgccga aagagggaac cgttgagccg ctgccgaacg gtaagcgtct gccgacagac
    11101 tttaatgaag gggcgaagaa caacacctat tctcgtaatg agaagatggt cggctacagc
    11161 ttcgatcacg aatttaacga cacctttact gtgcgtcaga acctgcgctt tgctgaaaac
    11221 aaaacctcgc aaaacagcgt ttatggttac ggcgtctgct ccgatccggc gaatgcttac
    11281 agcaaacagt gtgcggcatt agcgccagcg gataaaggcc attatctggc acgtaaatac
    11341 gtcgttgatg atgagaagct gcaaaacttc tccgttgata cccagttgca gagcaagttt
    11401 gccactggcg atatcgacca caccctgctg accggtgtcg actttatgcg tatgcgtaat
    11461 gacatcaacg cctggtttgg ttacgacgac tctgtgccac tgctcaatct gtacaatccg
    11521 gtgaataccg atttcgactt caatgccaaa gatccggcaa actccggccc ttaccgcatt
    11581 ctgaataaac agaaacaaac gggcgtttat gttcaggatc aggcgcagtg ggataaagtg
    11641 ctggtcaccc taggcggtcg ttatgactgg gcagatcaag aatctcttaa ccgcgttgct
    11701 gggacgaccg ataaacgtga tgacaaacag tttacctggc gtggtggtgt taactacctg
    11761 tttgataatg gtgtaacacc ttacttcagc tatagcgaat cgtttgaacc ttcttcgcaa
    11821 gttgggaagg atggtaatat tttcgcaccg tctaaaggta agcagtatga agtcggcgtg
    11881 aaatatgtac cggaagatcg tccgattgta gttactggtg ccgtgtataa tctcactaaa
    11941 accaacaacc tgatggcgga ccctgagggt tccttcttct cggttgaagg tggcgagatc
    12001 cgcgcacgtg gcgtagaaat cgaagcgaaa gcggcgctgt cggcgagtgt taacgtagtc
    12061 ggttcttata cttacaccga tgcggaatac accaccgata ctacctataa aggcaatacg
    12121 cctgcacagg tgccaaaaca catggcttcg ctgtgggctg actatacctt ctttgacggt
    12181 ccgctttcag gtctgacgct gggcaccggt ggtcgttata ctggctccag ctatggtgat
    12241 ccggctaact cctttaaagt gggaagttat acggtcgtgg atgcgttagt gcgttatgat
    12301 ctggcgcgag tcggcatggc gggctccaac gtggcgctgc atgttaacaa cctgttcgat
    12361 cgtgaatacg tcgccagctg ctttaacact tatggctgct tctggggcgc agaacgtcag
    12421 gtcgttgcaa ccgcaacctt ccgtttctaa tttctctttt ggggcacgga tttccgtgcc
    12481 catttcacaa gttggctgtt atgcaggaat acacgaatca ttccgatacc acttttgcac
    12541 tgcgtaatat ctcctttcgt gtgcccgggc gcacgctttt gcatccgctg tcgttaacct
    12601 ttcctgccgg gaaagtgacc ggtctgattg gtcacaacgg ttctggtaaa tccactctgc
    12661 tcaaaatgct tggccgtcat cagcctccgt cagaagggga gattcttctt gatgcccagc
    12721 cgctggaaag ctggagcagc aaagcgtttg cccgcaaagt ggcttatttg ccgcagcagc
    12781 ttcctccggc agaagggatg accgtgcgtg aactggtggc gattggtcgt tacccgtggc
    12841 atggcgcgct ggggcgcttt ggtgcggcag atcgcgaaaa ggtcgaggaa gctatctcgc
    12901 tggttggctt aaaaccgctg gcgcatcggc tggtcgatag tctctctggc ggcgaacgtc
    12961 agcgggcgtg gatcgccatg ctggtggcgc aggatagccg ttgtctgttg ctcgacgaac
    13021 cgacttcggc gctggatatc gcccaccagg ttgatgtgct ggcgctggtg caccgtttaa
    13081 gtcaggaacg tggcctgacg gtcattgccg tgctgcacga tatcaatatg gcggcacgct
    13141 actgtgatta tctggtcgcc ctgcgcggtg gtgaaatgat tgctcaggga acgcctgcgg
    13201 aaattatgcg cggcgaaacc ctcgaaatga tttatggcat cccgatgggt attttgccgc
    13261 atccggcggg tgctgcacct gtgagttttg tttattgatg agcggcttac ctcttatttc
    13321 gcgccgtcga ctgttaacgg cgatggcgct ttctccgttg ttatggcaga tgaataccgc
    13381 ccacgcggcg gctattgacc ccaatcgtat tgtggcgctg gagtggttgc cggtggaatt
    13441 actgctggcg ctcggcatcg tgccttacgg cgtggcggat accatcaact atcgcctgtg
    13501 ggtcagtgaa ccaccattgc cggactcagt gattgacgtc ggtttgcgta cagaacctaa
    13561 ccttgaactg ctgaccgaaa tgaaaccgtc gtttatggtc tggtcggcag gatatggccc
    13621 ttcatcagaa atgctggcac gtattgcgcc gggtcgcgga tttaacttca gtgacggcaa
    13681 acagccgttg gcgatggcgc gtaaatcgct gacggaaatg gcagatttac ttaacctgca
    13741 aagcgcagcg gaaacgcatt tagcgcaata cgaagacttt atcctcagca tgaaaccccg
    13801 ctttgtgaag cgtggtgcac gcccgttact gctgacgacg cttatcgatc cgcgccatat
    13861 gctggtcttc ggtccaaaca gcttgttcca ggaaattctc gatgagtacg gcatcccaaa
    13921 tgcctggcaa ggggaaacca acttctgggg cagtaccgcc gtcagtatcg atcgtctggc
    13981 ggcgtataaa gacgttgatg tgctctgttt tgatcacgac aacagcaaag acatggatgc
    14041 gctaatggca accccgctgt ggcaggccat gccgtttgtc cgtacagggc gctttcagcg
    14101 cgtacccgca gtctggtttt atggtgcaac gctttcggca atgcactttg tgcgtgttct
    14161 ggataacgcc atcggaggta aagcgtgagt aaacgaattg cgcttttccc ggcgttattg
    14221 ctggcgctgt tagtgattgt cgctacggcg ctaacctgga tgaacttctc acaggcgctg
    14281 ccgcgcagtc agtgggcgca ggccgcctgg tcgccggata ttgacgtcat cgagcagatg
    14341 atttttcact acagcttgtt gccgcgtctg gcgatttcgc tgctggtggg cgcaggcctg
    14401 gggctggtgg gcgtgctgtt tcagcaagtg ctgcgtaatc cgctggcgga accgacgaca
    14461 cttggcgttg ctacaggcgc gcaactggga attaccgtca ccacactttg ggcgatccca
    14521 ggtgctatgg cgagccagtt tgccgcactg gtgggcgctt gtgtcgttgg cttaatcgtc
    14581 tttggcgtcg cgtgggggaa acggctttcg ccggttacgc ttattctcgc ggggctggtg
    14641 gtgagccttt attgtggggc aattaatcag ctactggtta tcttccatca cgaccagctg
    14701 caaagcatgt tcctgtggag caccggaacg ctgacgcaaa ccgactgggg cggcgttgag
    14761 cgtttatggc cgcagttgct gggcggcgtg atgttgacgt tattgctact tcgcccgtta
    14821 accctgatgg ggcttgatga tggcgtggcg cgcaatctcg ggctggcctt gtcgctcgcg
    14881 cgtctggcag cgctgtcgct ggcgattgtc atcagtgcgc tgctggtgaa cgctgtgggg
    14941 attatcggct ttatcgggtt gttcgcgcca ctgctggcaa aaatgctggg ggcgcggcgt
    15001 ttattgccac ggttgatgct ggcgtcgctg attggtgcgt taattctgtg gctttctgat
    15061 caaatcatcc tctggctgac tcgcgtgtgg atggaagtgt ccaccggttc ggtcactgcg
    15121 ttgatcggtg cgccgctgct actgtggctg ctgccgcgtt tacggagcat tagcgcgccg
    15181 gatatgaagg tcaacgatcg tgtcgcgact gaacgccaac atgtgctggc gtttgtcctc
    15241 gcgggcggcg tgctgctgtt gatggctgtg gtagtggcgc tgtcgtttgg tcgtgatgcg
    15301 cacggctgga cgtgggcgag cggggcgttg cttgaggatt tgatgccctg gcgctggccg
    15361 cgaattatgg cggcgctgtt tgcgggcgtc atgctggcgg tggcgggctg tattattcag
    15421 cggctgaccg gaaacccgat ggcaagcccg gaagtgctgg ggattagctc tggcgcagcg
    15481 tttggcgtgg tgttgatgct gtttctggtg ccgggtaatg cctttggctg gctgttacct
    15541 gcaggcagtc tcggggcggc ggtgacgctg ttgatcatta tgatcgccgc cggacgcggt
    15601 ggattttccc cacaccgcat gttactggcg gggatggcgt taagtaccgc gttcaccatg
    15661 cttttgatga tgttgcaggc aagtggtgac ccgcgaatgg cgcaagtgct gacctggatt
    15721 tccggttcga cctacaacgc gaccgatgcg caggtctggc gcaccggaat tgtgatggtg
    15781 attttgctgg cgattacccc gctgtgccgc cgctggctga ccattttacc gctgggtggt
    15841 gataccgccc gagccgtagg aatggcgcta acgccgacgc gaattgcgct gctgctgtta
    15901 gcggcttgcc tgacggcgac cgcgacgatg actattggac cgttgagttt tgttggttta
    15961 atggcaccgc atattgcgcg gatgatgggc tttcgacgga cgatgccgca catcgtaatt
    16021 tcggcgctgg tgggtggttt actgctggtg ttcgctgact ggtgtgggcg gatggtgctg
    16081 tttccattcc agatcccggc ggggctgctg tcaaccttta tcggcgcgcc atattttatc
    16141 tatttgttga gaaagcagag ccgttaattg aatgcctcgc ccggtgggcg aggcaaaatc
    16201 atcacaactt cgcaaacacc cgacgtgcag catcgatggt gttattgata tcttccatgc
    16261 tgtgcgccac ggacataaag cccgcttcaa acgctgatgg tgccaggtaa acaccttcat
    16321 ccagcatcat atggaagaaa cgcttaaagc gttccacgtc acaggccatc acatcctgat
    16381 agcacgtcac ggactcggcg tcggtaaaga aaataccgaa catgccgcca acgtggttaa
    16441 cgaccagcgg aattccggct tcttctgccg cttccagcag accttctgcc agacgtgatg
    16501 tcagctcatc caacgtttcg tgaacgcccg gctgcgcgac ttcattcaga caggcgaaac
    16561 cggctgccat cgcaattggg ttaccggaaa gcgtacccgc ctgatagacc ggacccgtcg
    16621 gggccagcgc atccattaca tcacgacgac caccgaatgc gcctaccggc attccaccgc
    16681 cgatgatttt gccgaggcag gttaaatctg gcactacgcc gtaataatcc tgtgcaccag
    16741 ccagtgccac gcggaagcca gtcattactt catcgatgat cagcaatgcg ccaaattcgt
    16801 cgcacagcgc gcgcagacct ggcaggaact ctggcagcgg cggaacacag ttcatattgc
    16861 ctgccaccgg ctcaacgata atacaggcaa tctcttgcgg gtattgctca aacgcggcgc
    16921 gtacagaagc cagatcgtta taagtacagg ttaaggtatg tttggcgaaa tctgccggaa
    16981 cacccggcga gtttggctgg cctaacgtga gtgcgccaga accggctttc accagcaggc
    17041 agtcagcgtg accgtggtaa caaccttcaa atttaatgat tttgtcgcga ccggtaaaac
    17101 cacgggccag gcggatggcg ctcatcgtcg cttcagtgcc ggagttcacc atgcgcacca
    17161 tatccatggt cgggaccagt tcggtcacca gttgcgccat tttcacttcc atttcggtcg
    17221 gtgcaccaaa gcttaaacca cgctcggcgg cttcaatcac ggcattgcgg atagccggat
    17281 ggttatggcc cagcaccatc ggcccccagg aaccgacata atcgatatag gctttgccat
    17341 caacatcgta cagataagcg ccgtccgctt tttcgataaa cagtggagtg ccgcccacgc
    17401 cagtaaaggc gcgaacaggg gagttcacac cgccagggat cagctcgcgc gctgcgctgt
    17461 aaagattttc agacttactc atggagggtt cctgattcgt agaaaaagtg aatggctgct
    17521 attctatgtt attcataaca agttaaatac tcgtcaaaca tcaggctgct tgtactggtg
    17581 caatcctgat ttcttagagt ataaaagttt tgtgcatttg aaacattacg ctttgcaaag
    17641 gattttcatg gaacgtgcga gtaaaatgcc gtcatcttat ttgtatgacc aataagtgat
    17701 cattggatga aaactgatac tccctcttta gaaacaccgc aggccgcgcg cctgcgacgc
    17761 agacaactga ttcgccaact tcttgagcgc gataaaaccc cgttagccat tttgtttatg
    17821 gcggcagtcg tcggcacgct tgtcgggctg gctgcggttg cttttgacaa aggtgtcgcc
    17881 tggttgcaga accaacgtat gggggcgctg gtacatactg ctgataatta tccgctactg
    17941 ttaaccgtcg cttttctctg ttcggcggtg ctggcgatgt tcggctactt tttggtgcgc
    18001 aaatacgcgc ccgaagcagg gggatcgggg atcccggaaa ttgaaggggc gctggaagat
    18061 caacgtcctg ttcgctggtg gcgtgtattg ccggtgaaat tctttggcgg gctggggaca
    18121 ctcggcggag gcatggtgct agggcgcgaa ggaccaaccg tgcagatcgg cggcaatatc
    18181 ggtcgcatgg tgcttgatat tttccgcctg aaaggtgatg aagctcgcca tacgctgctg
    18241 gcaaccggtg ctgctgcggg gctggctgcg gcctttaacg cgccgctggc gggtattttg
    18301 tttattatcg aagagatgcg tccgcagttt cgctatacgt taatttcgat taaagcggta
    18361 tttattggcg tcattatgtc gaccattatg taccggattt ttaatcatga agttgcgttg
    18421 attgacgtcg gtaaactttc tgatgctccg cttaatacgc tgtggcttta tctgatcctc
    18481 ggtattattt ttggcatttt cggccctatt tttaataaat gggtgctggg gatgcaggat
    18541 ttgctgcacc gtgtgcacgg cggcaatatt accaaatggg tactgatggg tggtgcgatt
    18601 ggcggtctgt gtggattgct ggggtttgtg gcaccagcaa cgtcgggcgg cggttttaac
    18661 ctgattccta tcgctaccgc ggggaatttc agcatgggaa tgctggtgtt tatcttcgtc
    18721 gctcgggtca ttaccacctt actctgcttc tcttccggcg cgccgggcgg tatttttgcc
    18781 ccgatgctgg cgctgggtac tgtgctggga accgctttcg gaatggttgc cgttgagctg
    18841 tttccgcaat atcacctgga ggcggggacg tttgctattg ccggaatggg ggcattactg
    18901 gcggcatcta ttcgcgcgcc gttaacgggg atcattctgg ttctggagat gaccgataac
    18961 taccagctca ttttgccaat gattattacc ggtcttggcg caacactatt agcgcaattt
    19021 accggcggga aaccgctata ctcggcgatt cttgcgcgca cgctggcaaa acaggaagct
    19081 atgcaactgg cgcgaagcaa ggccgcatca gccagcgaga atacttgaac gaaataccag
    19141 ggtattagat aatggcgatt attattgggt tagaatttgc ccaattgccg atgtcgtttg
    19201 gagcaaaata tgagtgatga cgtagcactg ccgctggagt ttaccgacgc agcagccaac
    19261 aaagttaaaa gcctgatcgc tgacgaagat aacccgaatc tgaaattacg cgtgtatatc
    19321 accggtggcg gttgcagcgg cttccagtat ggtttcacct ttgatgatca ggtgaacgaa
    19381 ggcgatatga ccatcgaaaa acagggcgtt ggcctggtgg ttgatccgat gagcctgcaa
    19441 tatctggtcg gcggttccgt tgactatacc gaaggactgg aaggttctcg tttcatcgtg
    19501 accaacccga acgcgaaaag cacctgcggt tgcggttctt cctttagcat ctaatctgtt
    19561 gttcatgtgc cggataaact atccggcaca tcttctctct tcaacgccca ttctcatcca
    19621 gcgcaaacgt cggtagctta agatgccaac gaatagccgc cagccgaatc aagagcgtca
    19681 cgaccatgcc catcatactg gctgtttcca gtggtacgga aaatgtgtaa taagccgtag
    19741 cgtggacaat accgccgata atacaggcag ttgcgtagat ttctgtacgt aaaatcatgg
    19801 ggatttcgcg ggccagaaca tcacgaatga tcccgccgcc aacgccagta atgacgccca
    19861 tacaaaccgc gattaacgga ccggcttccg cattaaaggc tttattcacg ccaatgccga
    19921 caaacaccgc cagaccaacg gcgtccagca ccggcaacat ccattttggt aagcgtcttg
    19981 gctggcgcac cagcacgatg gtcagcatgc tggttaccat tgcaacgacc agatcggtgg
    20041 gatctttcac ccaaaatacc gggccgtgat ccagcgccat gtcgcgaatt gtcccgccgc
    20101 ctacagcggt aaccacgccc agtaccagaa caccaaaagg gtccatacgc aattttccgg
    20161 ctaacaaaac gccggagatg gcaaataccg ctgtgccgac tatatccagc caatagacga
    20221 gcattgttca atccccactg agcacctgaa aaggtcaggc attaatctac ctgtgaaagc
    20281 gcattacaga gctgttgtgc agcgaggata atacgtgggc ttgcacgttc aaaccagtca
    20341 ctcgtgagag gaataacggg aattttgagc tgttcacccc agtattgttt gattttagga
    20401 atttggtccg gtccgcctgt aatgacaatc gcctgtggcg agcgtgctaa cacctgttcg
    20461 cggctaactt gcggccaggg aacccggctg tctttaaaga tgttttctcc gccacaaact
    20521 tcgagtacct ggttctgaat cgactctttt ccactggtaa atggcggatt aatgccgaat
    20581 tgcagaaaaa cacgtttttt aggtttatca gcatattgcg ctttcaattg cgcgtactga
    20641 tccagcaggg attgcgcggc ttgttcggcc ttgtctggtt gcggactcca gggggccagt
    20701 tgacgtaacg cattggcaat ttgttcaatg cttgtcgcat cgacccacat cacttttatt
    20761 cccagcgaag ccagctggtc aacctgccgc tcggcattac ctccacgcca ggcaatcacc
    20821 agatcgggtt tcagcgcgac aatgcgttcc agattcatcc cctgccaggt ggaaacctgc
    20881 tcaatctttt gcgcttgtgg aggatagtcg gaatagctgc tgaccccaac cggcgtgatc
    20941 ccggcagcaa aggcaagttc agtgttggcg ggagaaagcg tgatgacgcg cggcgcggcg
    21001 ttgagccaca gtggcgcaag aaaagacagg gcgaccagcg ccctgaacag tgacttagcc
    21061 atgtgcaagt ttctgcacca gtgactcaac catcaggctg gactgtttag cggcaacagc
    21121 caggaactca tcgaagctaa gatgagactg ttgatcggcc acgtcggaga tggcgcgtac
    21181 gacaacaaac gggacgttga aattgtggca gacatgggcg attgccgtcg cttccatctc
    21241 tacagcaatg gcctgtggga agttgtggcg gattttcgcc agaccaacag aaccgttgat
    21301 gaaagcgtcg ccgctaacaa tcaggccacg tacagcgtta agattcagtt cggcaatgca
    21361 ggcctcagcg gcagcgatca gtttatcgtc agctttaaag cctgccggac agcctggtaa
    21421 ctgaccgtat tcataaccaa atgccgtgac atccgcgtcg tgataacgtg cttcgtccga
    21481 gacaacgata tcgcccactt tcaacgttgg tgccaggcca ccggcagaac cggtgttaat
    21541 aatcacatct ggcttgcagt gttccaacag caaagtggca cccagcgccg cagcgacttt
    21601 accgatgccc gatttcagaa gcgcaacctc ggttccattc agttggccgg tatagatttc
    21661 gcaaccgccg agactgatag tttgacggtt ttcgattttg tcacgcagca gcgtaacttc
    21721 ttcttccatt gcaccaatga tgccgatttt catagattta ctcgcgataa gcccgatttg
    21781 aaggcatagt ttaccatgcg cttacgggga agcgtatttc tcacgcggga gaggacatgg
    21841 cacagattga tttccgaaaa aaaataaact ggcatcgtcg ttaccgttca ccgcagggcg
    21901 ttaaaaccga acatgagatc ctgcggatct tcgagagcga tcgcgggcgt atcatcaact
    21961 ctccggcaat tcgtcgtctg caacaaaaga cccaggtttt tccactggag cgcaatgccg
    22021 ccgtgcgcac gcgtcttacc cactcgatgg aagtccagca ggtggggcgc tacatcgcca
    22081 aagaaatttt aagccgtctg aaagagctta aattgctgga agcatacggc ctggatgaac
    22141 tgaccggacc ttttgaaagc attgttgaga tgtcatgcct gatgcacgat atcggcaatc
    22201 cgccgtttgg tcattttggc gaagcggcga taaatgactg gtttcgccag cgtttatacc
    22261 cggaagatgc cgaaagccag cctctgactg acgatcgctg cagcgtggcg gcactacgtt
    22321 tacgggacgg ggaagaaccg cttaacgagc tgcggcgcaa gattcgtcag gacttatgtc
    22381 attttgaggg gaatgcacaa ggcattcgtc tggtgcatac attgatgagg atgaatctca
    22441 cctgggcaca ggttggcggt attttaaaat atacccgtcc ggcgtggtgg cgtggcgaaa
    22501 cgcctgagac acatcactat ttaatgaaaa agccgggtta ttatctttct gaagaagcct
    22561 atattgcccg gttgcgtaaa gaacttaatt tggcgcttta cagtcgtttt ccattaacgt
    22621 ggattatgga agctgccgac gacatctcct attgtgtggc agaccttgaa gatgcggtag
    22681 agaaaagaat atttaccgtt gagcagcttt atcatcattt gcacgaggcg tggggtcagc
    22741 atgagaaagg ttcgctcttt tcgctagtgg ttgaaaatgc ctgggaaaaa tcacgctcaa
    22801 atagtttaag ccgcagtacg gaagatcagt tttttatgta tttacgggta aacaccctaa
    22861 ataaactggt accctacgcg gcacaacgat ttattgataa tctgcctgcg attttcgccg
    22921 gaacgtttaa tcatgcatta ttggaagatg ccagcgaatg cagcgatctt cttaagctat
    22981 ataaaaatgt cgctgtaaaa catgtgttta gccatccaga tgtcgagcag cttgaattgc
    23041 agggctatcg ggtcattagc ggattattag agatttatcg tcctttatta agcctgtcgt
    23101 tatcagactt tactgaactg gtagaaaaag aacgggtgaa acgtttccct attgaatcgc
    23161 gcttattcca caaactctcg acgcgccatc ggctggccta tgtcgaggct gtcagtaaat
    23221 taccgtcaga ttctcctgag tttccgctat gggaatatta ttaccgttgc cgcctgctgc
    23281 aggattatat cagcggtatg accgacctct atgcgtggga tgaataccga cgtctgatgg
    23341 ccgtagaaca ataaccaggc ttttgtaaag acgaacaata aatttttacc ttttgcagaa
    23401 actttagttc ggaacttcgc gctataaaac gaatctgaag aacacagcaa ttttgcgtta
    23461 tctgttaatc gagactgaaa tacatgaaaa aaaccacatt agcactgagt gcactggctc
    23521 tgagtttagg tttggcgtta tctccgctct ctgcaacggc ggctgagact tcttcagcaa
    23581 cgacagccca gcagatgcca agccttgcac cgatgctcga aaaggtgatg ccttcagtgg
    23641 tcagcattaa cgtagaaggt agcacaaccg ttaatacgcc gcgtatgccg cgtaatttcc
    23701 agcagttctt cggtgatgat tctccgttct gccaggaagg ttcaccgttc cagagctctc
    23761 cgttctgcca ggggggccag ggcggtaatg gcggcggcca gcaacagaaa ttcatggcgc
    23821 tgggttccgg cgtcatcatt gatgccgata aaggctatgt cgtcaccaac aaccacgttg
    23881 ttgataacgc gacggtgatt aaagtccagc tgagcgatgg tcgtaagttc gacgcgaaga
    23941 tggttggcaa agatccgcgc tctgatatcg cgctgattca gatccagaac ccgaaaaacc
    24001 tgaccgcaat taagatggcg gattctgatg cactgcgcgt gggtgattac accgtagcga
    24061 ttggtaaccc gtttggcctg ggagagacgg taacttccgg gattgtctct gcgctggggc
    24121 gtagcggcct gaatgccgaa aactacgaaa acttcatcca gaccgatgca gcgatcaacc
    24181 gtggtaactc cggtggtgcg ctggttaacc tgaacggcga actgatcggt atcaacaccg
    24241 cgatcctcgc accggacggc ggcaacatcg gtatcggttt tgctatcccg agcaacatgg
    24301 tgaaaaacct gacctcgcag atggtggaat acggccaggt gaaacgcggt gagctgggta
    24361 ttatggggac tgagctgaat tccgaactgg cgaaagcgat gaaagttgac gcccagcgcg
    24421 gtgctttcgt aagccaggtt ctgcctaatt cctccgctgc aaaagcgggc attaaagcgg
    24481 gtgatgtgat cacctcactg aacggtaagc caatcagcag ctttgccgca ctgcgtgctc
    24541 aggtgggtac tatgccggtg ggcagcaaac tgaccctggg cttgctgcgc gacggtaagc
    24601 aggtcaacgt gaatctggaa ctgcagcaga gcagccagaa tcaggttgat tccagctcca
    24661 tcttcaacgg cattgaaggc gctgagatga gcaacaaagg caaagatcag ggcgtggtag
    24721 tgaacaacgt gaaaacgggc actccggctg cgcagatcgg cctgaagaaa ggtgatgtga
    24781 ttattggcgc gaaccagcag gcagtgaaaa acatcgctga actgcgtaaa gttcttgaca
    24841 gcaaaccgtc tgtgctggca ctgaacattc agcgcggcga cagcaccatc tacctgttaa
    24901 tgcagtaatc tccctcaacc ccttcctgaa tacgggaagg ggttctcctt acaatctgtg
    24961 aacttcgcca caactccata catcttcatc atcctttggg catttgcaca atgccgtacg
    25021 ttacgtactt ccttatgcta agccgtgcat aacggaggac ttatggctgg ctggcatctt
    25081 gataccaaaa tggcgcagga tatcgtggca cgtaccatgc gcatcatcga taccaatatc
    25141 aacgtaatgg atgcccgcgg gcgaattatc ggcagcggcg atcgtgagcg tattggtgaa
    25201 ttgcacgaag gtgcattgct ggtactttca cagggacgag tcgtcgatat cgatgacgcg
    25261 gtagcacgtc atctgcacgg tgtgcggcag gggattaatc taccgttacg gctggaaggt
    25321 gaaattgtcg gcgtaattgg cctgacaggt gaaccagaga acctgcgtaa atatggcgaa
    25381 ctggtctgca tgacggctga aatgatgctg gaacagtcgc ggttgatgca cctgttggcg
    25441 caggatagcc gtttgcggga agaactggtg atgaacctga ttcaggctga ggagaatact
    25501 cccgcactta ctgaatgggc gcaacggctg gggatcgatc tcaatcaacc gcgagtggtg
    25561 gctattgttg aggtcgacag cggtcagctt ggcgtggaca gcgcaatggc ggagttacaa
    25621 caactgcaaa atgcgctgac tacgcccgag cgtaataatc tggtggcgat tgtctcgcta
    25681 accgaaatgg tggtgttgaa accggcgttg aactcttttg ggcgctggga tgcagaagat
    25741 catcgtaagc gagttgaaca actgattacc cgcatgaaag agtacggcca gttgcgtttt
    25801 cgcgtttcgc tgggcaacta ttttaccggt cctggcagta ttgcccgatc ctatcgtacg
    25861 gcgaaaacga cgatggtggt gggtaaacag cggatgccag aaagtcgctg ctatttttat
    25921 caggatctga tgttacctgt gttactcgac agtttgcgtg gcgactggca ggccaacgaa
    25981 ctggcgcgac cgctggcgcg gctgaaagcg atggacaata acggcttgct gcgacgaacg
    26041 ctggcggcgt ggtttcgtca caatgtgcaa ccgctggcaa cgtcaaaggc gttgtttatt
    26101 catcgtaata ccctggagta tcggcttaat cgtatatcgg aactgaccgg gcttgatttg
    26161 ggcaattttg atgacaggtt gctgctgtat gtggcgttac aactggatga agagcggtag
    26221 gttatgcgtt aaggttgagg cggcgctggc tcattcccct cagcccgaag gggagagggg
    26281 aatgcccgaa ttaaccgtca attcgggatt atttacgcgt tagtttttcc agatcggctt
    26341 caatctcgct gatcttattg gttacgactg actccaggtg acgtaagtcg tcgaggatct
    26401 tacgcttaag atcaacttcg ctgcggtcac gctggcagat ttgatcaagc tcatcaatga
    26461 tataccgtag attcgggctg atttcctgga cttctttata accctgaccc acaccatcag
    26521 cgacgaccgt tttacgctga cgcggatatt taaacttaac gctcttggcg aaaaactcgc
    26581 ctttgtcttt ctggaaatag attttcagaa tatcgttgtt ggcttcctgc cggaggctgt
    26641 aacgatcaat ttcttcagga ttggtaatac ccagactttt cagattgtcg tacatagcgt
    26701 tacctcaaaa tgagtcagta aattgtgctt attttagcat ttggcctggc ccgcccggct
    26761 cgactgtgat cgcagaaagc ctttttcttc cagacttcta tcatggcgta aaaatcaaaa
    26821 aattacctgc tttattgtgg cgatattcca tgggatggat taacacattt atcttcgttt
    26881 tgcgaattac atgacagtcc gaaacaagat gaaaaagtat ttaaaaaagg ggggaagaag
    26941 aatgagtagg acgaattatt ctgagccaga aagcatacgg gcagcatcat gctgcccgta
    27001 tatgtactct taatcgatgg tacgcagcag ttcgttaatg ccgactttgc cgcgagtttt
    27061 cgcgtcaact ttcttaacga taaccgcaca gtagaggctg tatttgccat ctttcgacgg
    27121 cagattacct gaaacaacca cagaccccgc cggaacgcga ccgtagtgga tttcgccggt
    27181 ttcacggtcg taaatacggg tgctctgacc aatgtatacg cccatagaga tgacggaacc
    27241 ttcttcgaca atcacccctt caaccacttc ggagcgtgcg ccaatgaagc agttatcttc
    27301 aataatggtc gggttagcct gcagcggctc cagcacgccg ccgatgccca cgccaccgga
    27361 aaggtggacg tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg tatcaaccat
    27421 cgtgccttca tcaacatatg cgccgatgtt gacgtaagac ggcatcagca cggtgttacg
    27481 ggcaataaat gcaccctgac gtaccgccgc tggtggcaca acgcggaagc cttctttctg
    27541 aaaacgcgct tcgtcgtagt cggcgaattt catcggcact ttatcgaagt agcggctttc
    27601 tgccccttcg atcacctgat tatcattaat acggaaagag agcagcaccg cttttttcaa
    27661 ccactgatgc gtcacccact gaccgtcaat tttttccgct acacgcagtg cgccggaatc
    27721 cagcagggcg atcacctgat ttaccgcttc gcgggttacg gtgtctgcat tggctggcgt
    27781 gatctcggca cggcgttcaa aagcggtttc aataatgttc tgtaactgct gcattgttaa
    27841 actcttttca tatcagtaaa cacatcaccc tttatcgttt ggattgaggg cctctgtcaa
    27901 ccgctgatgc acttcctgct gcaactcgtt attaagtgca cgccggtcag cggtggcaat
    27961 tatgaataaa tcttctactc gctcgccaat ggttgtaatt cgggcaccat gaagcgaaat
    28021 tcccagatcg gcaaaaattt tcccgactct cgccagcagt cctggctggt cgagcgcgat
    28081 aagctcaagg aacgatttgc ggtcggtatg ggtcggcaaa aacgttactt cggtttcaac
    28141 agtaaaatgg cgtaatttgg cgggttggcg acggggctgc ggtggctgcc agctactttg
    28201 cgtcagtact tgctccagac caaaccgaat aacctcatga cgatctgcgg acagcgggct
    28261 gccatcgggt tccagcacga taaaggtatc catcgccata ccgtcgcgag tggtgaaaat
    28321 ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg gcacagacgg cggcaaacag
    28381 ataggggcgg tccgggctcc agataaaaat ctcggtgcct ccacgcgtag cctgcgggct
    28441 aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa tggcgggcat gccaggccag
    28501 ttgatttggg ctatggcgga caaaatagtt agcacgacag cgtgaccaaa tttggtgcag
    28561 cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt gcctggagtt ggtgatggcg
    28621 aacccgttcg cgcatatccg gcgtgttttg cattccgcgt cgtagctgct tttcggtggc
    28681 aaaatagagt tcgcgcaaca gactttgctt ccagctattc cacagcgttt cgttggtggc
    28741 gcaaatgtca gccactgtca ggcataccag atagcgcaga cgattttccg tttgaacttc
    28801 ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg cggcgttgtg ctgtcaccga
    28861 catcagcaag tgctggcgaa ccagccaggc aaccagctgt gtttcgcgtg agttcagccc
    28921 gtggagttct gcaaaatgca ctacatcctg agcaccgaga atggagtggt cgccgccgcg
    28981 tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc agctcagttg acggcaggcg
    29041 cggccagacg tccacacaca acggatggcg ctggcgcgtt tcttcactgg caaaactctc
    29101 cagtttcagc atcacgcgga tagtatgttc atccaccgtg taggcgtgga acagatcaaa
    29161 ctgcatctgc ccgacgatat gcgaccattg cggcatatac gcgccgagca cgctatggcg
    29221 atgcattggc aatagcccgc gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag
    29281 ttttcgtgct tccggaatat tacacagcgg ttgttgcaga tggcgacggg catggcgtaa
    29341 ctggcgcagc gtggtggagt aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa
    29401 catacgcaag atggcttccg gctggcgcat aaatagtgtt tcatcacgca ggtcgattag
    29461 cgtaccgcgt agctgaaact catcgtcgat tggacgtggt ttttcgtcgg cgggaagggc
    29521 gaggatggct tcatcgaaca gttgcagcag catctggttg agttcactga cgcggcgtgt
    29581 aacgcggaag taatccttca tcatccgctc gaccggttcg ttgccttcac cactgtaatt
    29641 cagacgctga gcgacgctaa gttggcgatc gaataacaga cgattatcgt aacggctgac
    29701 gaccagatgc agggcaaagc gaatacgcca cagtatatgc agacattcgt ttaattccgc
    29761 ccgctccgct gaggttaaga agccaaaccc gaccatttca tccagcgatg ttgcgccaaa
    29821 atgacggcgg gccacccatt gcagagtgtg aatatcgcgc aagccgccag ggctactttt
    29881 gatgtctggt tcaaggttgt agctggtgcc atggtaacgc tgatggcgct ggttctgttc
    29941 ttcaactttc gccgcgtaga acttgtcgga aggccagaat ccttcgctaa aaatatgttt
    30001 ttgcagttcg aggaagagcg caacatcgcc aattaataag cgggattcga ttaaattggt
    30061 ggcgacggtt aaatccgata acccttccag catgcactct tcaagcgtgc gcacactatg
    30121 accgacttcc agctttacat cccagagcag cgttaacagc tcgcccactt tttgcgcctg
    30181 atcgtccggg agctttttac ggcttaaaat cagtaaatcg atgtcagaaa gtggatgcag
    30241 ctcgccacga ccgtagccac cgacggcgac caatgccagg tcggcaatct ggctgaatcc
    30301 cgcttcaatc cataatcgtt gcaggagctg gtcgataaac tcggtgcgcg cctcaatcaa
    30361 ctgttctgca gagatcccat tgtcaaaggc atcacccagc caacgctgga aagtatcgat
    30421 atgggctttt atcccaccga cggttaattc atcacggggc cagacgcatg gattttgcgg
    30481 ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt tctggaaggg tattcattgt
    30541 gcgccaccca taagattaaa ttatcgcatt aaaaaagccg gcattcgccg gcttcatctt
    30601 attcgtcgtg cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc aaaatttcgc
    30661 agccgttatc agtcaccaca atagtatgct catattgtgc agacaagctg cgatctttgg
    30721 tttttaccgt ccagccatct ttcatggtgc ggatctcttt tttacccgcg ttgaccattg
    30781 gctcaatggt gaacgtcatc cccggtttca gcacgacgtt ggtttcacgg gagtcatagt
    30841 gcagcacctg cggttcttca tggaagccgc gaccaatacc gtgtccacaa tattcacgaa
    30901 cgacggagaa gccttctgct tcgacaaact tctgaatcgc cgcaccgatt tcgcgcaggt
    30961 taatgcctgg tttgaccatg cgcagcgcca ggtacaggct ttcttgcgtg atgcggcaca
    31021 gacgttcgcc catgatggtc ggcttaccga cgataaacat tttcgaggta tcgccgtgga
    31081 aaccatcttt gattacggtg acatcaatgt taacgatatc gccatctttc agcagcttag
    31141 catcgtccgg gataccgtgg cacaccactt cattaataga gatgcaaacg gatttcggat
    31201 agccgtgata gccgaggcag gcagaaaccg cgtgttgttc attaacaatg taatcattac
    31261 agatgcgatc cagctcgccg gtgctgacgc ccggtttaac atacggttcg atcatctcca
    31321 gcacttcggc agccagtcgg ccagcgacgc gcattttttc gatatcttct ggggtcttga
    31381 ttgagatagc cattaattct gtccatcagc gtcggtgata ccgacaatat atatgtaagt
    31441 gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc
    31501 cactgaaggt tttctataat agaaaattcg acatctgatg ctgtacacag cgccaacaat
    31561 tattggtgtc catgacgtat ttgtggtata aagcgcgccg gacttccgat ccatttcgta
    31621 tacacagact ggacggaagc gacaatctca ctttgtgtaa caacacacac gtatcggcac
    31681 atattccggg gtgccctttg gggtcggtaa tatgggatac gtggaggcat aaccccaact
    31741 tttatataga ggttttaatc atggcaactg tttccatgcg cgacatgctc aaggctggtg
    31801 ttcacttcgg tcaccagacc cgttactgga acccgaaaat gaagccgttc atcttcggtg
    31861 cgcgtaacaa agttcacatc atcaaccttg agaaaactgt accgatgttc aacgaagctc
    31921 tggctgaact gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt ggtactaaac
    31981 gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc
    32041 atcgctggct gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc
    32101 tgaaagacct ggaaactcag tctcaggacg gtactttcga aaagctgacc aagaaagaag
    32161 cgctgatgcg cactcgtgag ctggagaaac tggaaaacag cctgggcggt atcaaagaca
    32221 tgggcggtct gccggacgct ctgtttgtaa tcgatgctga ccacgaacac attgctatca
    32281 aagaagcaaa caacctgggt attccggtat ttgctatcgt tgataccaac tctgatccgg
    32341 acggtgttga cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc
    32401 tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca ggatctggct tcccaggcgg
    32461 aagaaagctt cgtagaagct gagtaataag gcttgataac tcccccaaaa tagttcgagt
    32521 tgcagaaagg cggcaagctc gagaattccc gggagcttac atcagtaagt gaccgggatg
    32581 agcgagcgaa gataacgcat ctgcggcgcg aaatatgaag ggggagagcc cttatagacc
    32641 aggtagtaca cgtttggtta gggggcctgc atatggcccc ctttttcact tttatatctg
    32701 tgcggtttaa tgccgggcag atcacatctc cgaggatttt agaatggctg aaattaccgc
    32761 atccctggta aaagagctgc gtgagcgtac tggcgcaggc atgatggatt gcaaaaaagc
    32821 actgactgaa gctaacggcg acatcgagct ggcaatcgaa aacatgcgta agtccggtgc
    32881 tattaaagca gcgaaaaaag caggcaacgt tgctgctgac ggcgtgatca aaaccaaaat
    32941 cgacgtcaac tacggcatca ttctggaagt taactgccag actgacttcg ttgcaaaaga
    33001 cgctggtttc caggcgttcg cagacaaagt tctggacgca gctgttgctg gcaaaatcac
    33061 tgacgttgaa gttctgaaag cacagttcga agaagaacgt gttgcgctgg tagcgaaaat
    33121 tggtgaaaac atcaacattc gccgcgttgc tgcgctggaa ggcgacgttc tgggttctta
    33181 tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa ggcgctgacg aagagctggt
    33241 taaacacatc gctatgcacg ttactgcaag caagccagaa ttcatcaaac cagaagacgt
    33301 atccgctgaa gtggtagaaa aagaatacca ggtacagctg gatatcgcga tgcagtctgg
    33361 taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc atgaagaaat tcaccggcga
    33421 agtttctctg accggtcagc cgttcgttat ggaaccaagc aaaactgttg gtcagctgct
    33481 gaaagagcat aacgctgaag tgactggctt catccgcttc gaagtgggtg aaggcatcga
    33541 gaaagttgag actgactttg cagcagaagt tgctgcgatg tccaagcagt cttaattatc
    33601 aaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat cttgtaaatt cagctaaccc
    33661 ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag tattaattca tttcaatcgt
    33721 tgacagtctc aggaaagaaa catggctacc aatgcaaaac ccgtctataa acgcattctg
    33781 cttaagttga gtggcgaagc tctgcagggc actgaaggct tcggtattga tgcaagcata
    33841 ctggatcgta tggctcagga aatcaaagaa ctggttgaac tgggtattca ggttggtgtg
    33901 gtgattggtg ggggtaacct gttccgtggc gctggtctgg cgaaagcggg tatgaaccgc
    33961 gttgtgggcg accacatggg gatgctggcg accgtaatga acggcctggc aatgcgtgat
    34021 gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg ctattccatt gaatggcgtg
    34081 tgcgacagct acagctgggc agaagctatc agcctgttgc gcaacaaccg tgtggtgatc
    34141 ctctccgccg gtacaggtaa cccgttcttt accaccgact cagcagcttg cctgcgtggt
    34201 atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag ttgacggcgt gtttaccgct
    34261 gatccggcga aagatccaac cgcaaccatg tacgagcaac tgacttacag cgaagtgctg
    34321 gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc tggctcgtga ccataaatta
    34381 ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc gccgtgtggt aatgggtgaa
    34441 aaagaaggga ctttaatcac ggaataattc ccgtgatgga taaataaggg taagattccg
    34501 cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt attgttgcag gcagtttggt
    34561 cacggccagc gcgcagcaac cggagcgtac aaaagtacgt gaggatggcg agcactgctc
    34621 ggggccaaaa tggcaaataa aatagcctaa taatccagac gattacccgt aatatgttta
    34681 atcagggcta tacttagcac acttccactg tgtgtgactg tctggtctga ctgagacaag
    34741 ttttcaagga ttcgtaacgt gattagcgat atcagaaaag atgctgaagt acgcatggac
    34801 aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac gcacgggtcg tgcttctccc
    34861 agcctgctgg atggcattgt cgtggaatat tacggcacgc cgacgccgct gcgtcagctg
    34921 gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca acgtgtttga tcgttcaatg
    34981 tctccggccg ttgaaaaagc gattatggcg tccgatcttg gcctgaaccc gaactctgcg
    35041 ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag aacgtcgtaa agatctgacc
    35101 aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag tacgtaacgt gcgtcgtgac
    35161 gcgaacgaca aagtgaaagc actgttgaaa gataaagaga tcagcgaaga cgacgatcgc
    35221 cgttctcagg acgatgtaca gaaactgact gatgctgcaa tcaagaaaat tgaagcggcg
    35281 ctggcagaca aagaagcaga actgatgcag ttctgatttc ttgaacgaca aaaacgccgc
    35341 tcagtagatc cttgcggatc ggctggcggc gttttgcttt ttattctgtc tcaactctgg
    35401 atgtttcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt gcagcacgct
    35461 ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg caggcaaaaa
    35521 tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg taatggacga
    35581 tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc gcaccgaagt
    35641 cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg atcaggtgat
    35701 ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc gcgcgggtaa
    35761 aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt ttatggacgc
    35821 cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg ccatttttca
    35881 gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc aaaatggcgt
    35941 ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat tgcgcgattt
    36001 ggcaacaatg acgccggatc aagcctgccg tcatccgaac tggtcgatgg ggcgtaaaat
    36061 ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag cgcgttggct
    36121 gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag tgattcactc
    36181 aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg atatgcgtac
    36241 gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga agccgctcga
    36301 tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt atccatgcct
    36361 gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg acgacagcat tgaatgccgc
    36421 aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg atatcgctgc
    36481 gttgaattta tccgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg tggacgatgt
    36541 gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc gtctcgcaag
    36601 ctgaggataa tccggctaca gagagtcgcg ctatttgtta gcgtagggct tcagtgatat
    36661 agtctgcgcc atctgatcgt aagtagttgg ctttataagg tcaggtatgc cgtggtttta
    36721 cacggctttt ttttgtatag gcttcagtat tcctgagtac cgtaaaccct gtcagggaat
    36781 aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg aaaaattgcc agcgcatggc
    36841 tgccgtcatg ttgcgatcat tatggacggc aatggtcgct gggcaaaaaa gcaagggaag
    36901 attcgtgcct ttgggcataa agccggggca aaatccgtcc gccgggctgt ctcttttgcg
    36961 gccaacaacg gtattgaggc gttaacgctg tatgccttta gtagtgaaaa ctggaaccga
    37021 ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt gggcgctcga tagcgaagta
    37081 aaaagtctgc accgacataa cgtgcgtctg cgtattattg gcgataccag tcgctttaac
    37141 tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa cagccgggaa taccggtctg
    37201 acgctgaata ttgcggcgaa ctacggtgga cgttgggata tagtccaggg agtcaggcaa
    37261 ctggctgaaa aggtgcagca aggaaacctg caaccagatc agatagatga agagatgcta
    37321 aaccagcatg tctgtatgca tgaactggcc cctgtagatt tagtaattag gactgggggg
    37381 gagcatcgca ttagtaactt tttgctttgg caaattgcct atgccgaact ttactttaca
    37441 gatgttctct ggcccgattt cgatgaacaa gactttgaag gggcgttaaa tgcctttgct
    37501 aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg aaacagcctg atgggggtcg
    37561 cttttgctga agtatcgcct gatatctgct tttgtgttaa tacccgtcgt catcgcggcg
    37621 ttgtttctgt tgccgccggt ggggttcgcc atcgtaacgc tggtggtctg catgctggca
    37681 gcgtgggaat ggggacagct tagcggtttt accactcgtt cgcagcgagt atggttggcg
    37741 gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt tgccggaata tcaccgaaat
    37801 attcatcaac cgctggttga aatctcactt tgggcttcgc tgggttggtg gattgtcgcg
    37861 ctattgctgg tgctgtttta cccaggttcc gcagcaatct ggcgtaactc taaaacattg
    37921 cgccttattt ttggcgtgct aaccattgtt cccttcttct ggggcatgct ggcgttacgg
    37981 gcctggcact acgacgagaa tcattacagt ggcgcaatat ggctgctcta tgtcatgatc
    38041 ctggtatggg gcgctgactc tggcgcatat atgtttggca aattgtttgg taaacataag
    38101 ctggcaccga aggtttctcc gggtaaaacc tggcaaggct ttataggtgg actagcgacc
    38161 gcagcggtaa tctcatgggg ttatggcatg tgggcgaatc tcgacgtcgc tcccgtcacc
    38221 ttactcattt gctctattgt cgcagcgtta gcctcagtgc tcggcgatct gaccgagagt
    38281 atgtttaagc gtgaagcagg aattaaggac agcggtcatt taattccagg acacggtggt
    38341 attttagatc gtattgatag cctgacggct gcggtaccgg tctttgcttg cttgttgtta
    38401 ctggtattca ggacgcttta acggaaggta atatgctgag ttttctctgg gatttggctt
    38461 cgttcatcgt tgcactgggt gtacttatca ccgtgcatga atttggtcat ttctgggttg
    38521 cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg gtttggtaag gcgctctggc
    38581 ggcgaactga taagctcggc accgaatatg ttatcgccct gatcccgctg ggcggttatg
    38641 tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga actccgccac catgccttca
    38701 ataataaatc tgttgaccaa cgagcggcga ttattgccgc aggtccggtt gcaaacttca
    38761 tttttgctat ctttgcctac tggctggttt ttattattgg tgtgcctggc gtacgtccgg
    38821 tggttggtga aatagcagcc aattcgatag ctgcggaagc acaaattgca ccaggtacgg
    38881 aactaaaagc cgtagatggt atcgaaacgc ctgattggga tgccgtgcgt ttgcagttgg
    38941 tcgataaaat tggcgatgaa agcaccacca ttacagtagc gccatttggc agcgaccaac
    39001 ggcgggatgt aaagctcgat ttacgtcact gggcgtttga gcctgataaa gaagatccgg
    39061 tatcttcgct ggggattcgt cctcgtgggc cgcaaattga acctgtactg gaaaatgtgc
    39121 agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg cgacaggatc gttaaagtcg
    39181 atggtcagcc cttaacgcag tgggtgacct ttgtgatgct tgtccgggat aacccgggta
    39241 aatccttagc gttagaaatc gaaaggcagg ggagtccctt gtctttgaca ttaatcccgg
    39301 agagtaaacc gggtaatggt aaagcgattg gttttgtcgg tattgagccg aaagtcattc
    39361 ctttgccaga tgagtataaa gttgtacgcc agtatgggcc gttcaacgcc atcgtcgaag
    39421 ccacggacaa aacgtggcag ctgatgaagc tgacggtcag tatgctggga aaattgatca
    39481 ccggtgatgt gaaactgaac aacctcagtg ggccgatctc tatcgccaag ggggctggga
    39541 tgacagcgga actcggggta gtttattacc tgccgtttct tgcgcttatt agcgtgaact
    39601 tagggataat taacctgttt ccgttgcccg tacttgacgg ggggcatctg ctgttccttg
    39661 cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt tcaagacttt tgttatcgca
    39721 ttggctcgat tctgctggtg ctgttaatgg ggcttgcact tttcaatgat ttctctcggt
    39781 tatgagagtt agttaggaag aacgcataat aacgatggcg atgaaaaagt tgctcatagc
    39841 gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa gggttcgtag tgaaagatat
    39901 tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggcc ctcctcagta tgccggtgcg
    39961 cacaggcgac acggttaatg atgaagatat cagtaatacc attcgcgctc tgtttgctac
    40021 cggcaacttt gaggatgttc gcgtccttcg tgatggtgat acccttctgg ttcaggtaaa
    40081 agaacgtccg accattgcca gcattacttt ctccggtaac aaatcggtga aagatgacat
    40141 gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc gaatccctcg atcgcaccac
    40201 cattgccgat atcgagaaag gtctggaaga cttctactac agcgtcggta aatatagcgc
    40261 cagcgtaaaa gctgtcgtga ccccgctgcc gcgcaaccgt gttgacctaa aactggtgtt
    40321 ccaggaaggt gtgtcagctg aaatccagca aattaacatt gttggtaacc atgctttcac
    40381 caccgacgaa ctgatctctc atttccaact gcgtgacgaa gtgccgtggt ggaacgtggt
    40441 aggcgatcgt aaataccaga aacagaaact ggcgggcgac cttgaaaccc tgcgcagcta
    40501 ctatctggat cgcggttatg cccgtttcaa catcgactct acccaggtca gtctgacgcc
    40561 agataaaaaa ggtatttacg tcacggtgaa catcaccgaa ggcgatcagt acaagctttc
    40621 tggcgttgaa gtgagcggca accttgccgg gcactccgct gaaattgagc agctgactaa
    40681 gatcgagccg ggtgagctgt ataacggcac caaagtgacc aagatggaag atgacatcaa
    40741 aaagcttctc ggtcgctatg gttatgccta tccgcgcgta cagtcgatgc ctgaaattaa
    40801 cgatgccgac aaaaccgtta aattacgcgt gaacgttgat gcgggtaacc gtttctacgt
    40861 gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat gccgtcctgc gtcgcgaaat
    40921 gcgtcagatg gaaggtgcat ggctgggaag cgatctggtc gatcagggta aggagcgtct
    40981 gaatcgtctg ggcttctttg aaactgtcga taccgatacc caacgtgttc cgggtagccc
    41041 ggaccaggta gatgtcgtct acaaggtaaa agagcgcaac accggtagct tcaactttgg
    41101 tattggttac ggtactgaaa gtggcgtgag cttccaggct ggtgtgcagc aggataactg
    41161 gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa aacgattacc agacctatgc
    41221 tgaactgtcg gtaaccaacc cgtacttcac tgtagatggt gtaagcctcg gtggtcgtct
    41281 cttctataat gacttccagg cagatgacgc cgacctgtcc gactatacca acaagagtta
    41341 tggtacagac gtgacgttgg gcttcccgat caacgaatat aactcgctgc gtgcaggtct
    41401 gggttatgta cataactccc tgtccaacat gcagcctcag gttgcgatgt ggcgttatct
    41461 gtactctatg ggtgaacatc cgagcacctc tgatcaggat aatagcttca aaacggacga
    41521 cttcacgttc aactatggtt ggacctataa caagcttgac cgtggttact tcccgacaga
    41581 tggttcacgt gtcaacctga ccggtaaagt gaccattcct ggatcggata acgaatacta
    41641 caaagtgacg ttagacacgg cgacttatgt gccgatcgat gacgatcaca aatgggttgt
    41701 tctggggcgt acccgctggg gttatggtga tggtttaggc ggcaaagaga tgccgttcta
    41761 cgagaacttc tatgccggtg gttccagcac cgtgcgtggc ttccagtcca atacaattgg
    41821 tccgaaagca gtttacttcc cgcatcaggc cagtaattat gatccggact atgattacga
    41881 atgtgcgact caggacggcg cgaaagacct gtgtaaatcg gatgatgctg taggcggtaa
    41941 cgccatggcg gttgccagcc tcgagttcat caccccgacg ccgtttatta gcgataagta
    42001 tgctaactcg gttcgtactt ccttcttctg ggatatgggt accgtttggg atacaaactg
    42061 ggattccagc caatattctg gatatccgga ctatagtgat ccaagcaata tccgtatgtc
    42121 tgcgggtatc gcattacaat ggatgtcccc attggggccg ttggtgttct cctacgccca
    42181 gccgttcaaa aagtacgatg gagacaaggc agaacagttc cagtttaaca tcggtaaaac
    42241 ctggtaagtg ttctccacaa aggaatgtag tggtagtgta gcgatgactt taggcgatca
    42301 atataagatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg gatggtaagg
    42361 agtttattgt gaaaaagtgg ttattagctg caggtctcgg tttagcactg gcaacttctg
    42421 ctcaggcggc tgacaaaatt gcaatcgtca acatgggcag cctgttccag caggtagcgc
    42481 agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc agcgaactgc
    42541 agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg aaagcgggca
    42601 gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt gctcagaaag
    42661 cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga agaacgcggc aaactggtta
    42721 ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat ctggttgttg
    42781 atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc gacgtactga
    42841 aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag cagttggatg
    42901 cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg caatctgcac
    42961 aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta ggcttgtgcc
    43021 aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt gccgcgctgg
    43081 tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat accacgccgc
    43141 agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag ctgggtaaca
    43201 acgtatcgat tggcgctaac gcggtgattg agtccggcgt tgaactgggc gataacgtga
    43261 ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt tcgcgtctct
    43321 gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg atccagtccg
    43381 gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac tgggtgaaga
    43441 tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc tgcacaacca
    43501 tcgatcgcgg cgcgctggat gacactatta ttggcaatgg cgtgatcatt gataaccagt
    43561 gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt ggcgtcatta
    43621 tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc gtaatcaacg
    43681 ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg atgcgtccca
    43741 tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa gtctggcgca
    43801 aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa tcgcttgagc
    43861 gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt acggcctgtc
    43921 tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt ttgacaggaa
    43981 gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag aacttctgcc
    44041 gcaccgtttc cctttcttac tggtggatcg cgtgctggat tttgaagaag gtcgttttct
    44101 gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt tccctggaaa
    44161 accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag gtattctggc
    44221 gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tatttcgctg gtattgacga
    44281 agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag tcactttcga
    44341 aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgacg gtaaagtagt
    44401 ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga ttgataaatc
    44461 cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcgattggcg cgaacgctca
    44521 cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta ccgtactgaa
    44581 atctcacgtt gtcgtgaatg gtcatactaa aattggccgc gataatgaga tttatcagtt
    44641 cgcctccatc ggcgaagtta accaggatct gaaatatgct ggcgaaccga cccgcgtgga
    44701 aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca cagtccaggg
    44761 cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaatgcgc acattgcgca
    44821 cgattgtacg gtaggtaacc gctgcatcct cgccaacaac gcaacgctgg cgggtcacgt
    44881 atcggttgac gacttcgcga tcatcggcgg catgaccgca gtccatcagt tctgcatcat
    44941 tggtgcgcat gtgatggttg gcggctgctc cggtgtggcg caggacgtcc ctccttatgt
    45001 cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat atcgaagggc tgaagcgccg
    45061 cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg tataagctga tttatcgtag
    45121 cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa catatccgga
    45181 agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc gttaatgact
    45241 gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga tatcctgggg
    45301 gccggtttaa tccgcgctct gaaagaacgt gtgcccaacg cccgctttgt tggtgttgcc
    45361 gggccacgaa tgcaggctga aggctgcgaa gcctggtacg aaatggaaga actggcagtg
    45421 atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tacatattcg tgccgatctg
    45481 acaaagcgtt ttggcgaact gaagccagat gtttttgttg gtattgatgc gcctgacttc
    45541 aatattactc ttgaaggtaa cctcaaaaag cagggtatca aaaccattca ttacgtcagt
    45601 ccgtccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc caccgatctg
    45661 gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca aatacaacgt accgtgccgc
    45721 tttatcggtc ataccatggc tgatgccatg ccattagatc cagataaaaa tggtgcccgt
    45781 gatgtgctgg ggatccctta cgatgcccac tgtctggcat tgttgccggg cagccgtggt
    45841 gcagaagtcg aaatgcttag tgccgatttc ctgaaaacgg cccagctttt gcgccagaca
    45901 tatccggatc tcgaaatcgt ggtgccgctg gtgaatgcca aacgccgcga gcagtttgaa
    45961 cgcatcaaag ctgcagtcgc gccagacctg tcggttcatt tgctggatgg aatgggccgt
    46021 gaggcgatgg tcgccagtga tgcggcgcta ctggcgtcgg gtacggcagc actggagtgt
    46081 atgctggcta aatgcccgat ggtagtggga tatcgcatga agcctttcac cttctggctg
    46141 gcgaagcgac tggtgaaaac tgattatgtc tcgctgccaa atctgctggc gggcagagag
    46201 ttagtcaaag agttattgca ggaagagtgt gagccgcaaa aattggctgc ggcgctgtta
    46261 ccgctgttgg cgaacgggaa aaccagccat gcgatgcacg acaccttccg cgagctgcat
    46321 cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga gttagcacaa
    46381 tgatcgaatt tgtctatccg cacacgcagc tggttgcggg tgtggatgaa gtcggacgcg
    46441 ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct tgacccggcg cgcccaattg
    46501 ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg tctggcgctc tgtgaagaga
    46561 tcaaagagaa agcgttgagc tggagtctgg gccgtgcgga acctcacgaa atcgacgaac
    46621 tgaatatttt acatgcgact atgctggcga tgcagcgtgc cgtcgctgga ctgcatattg
    46681 cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg ccttcgatgg
    46741 ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc ctggcgaaag
    46801 tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat ggttttgccc
    46861 aacacaaagg gtatccaacc gcttttcatc tggaaaaact ggctgaacac ggcgcgaccg
    46921 aacaccatcg gcgcagcttt gggcctgtca aacgcgcact gggacttgcg tcctgattct
    46981 tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc gtacacctgc
    47041 gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg ctggtaaaaa
    47101 aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac ctttgcggtc
    47161 tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcccatcgtc ggggcggatt
    47221 ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca cctgacggtg ctggcggcga
    47281 acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag cgcgggtacg
    47341 gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaatgaa gggttgatcc
    47401 ttctttccgg cgggcgcatg ggcgacgttg gacgcagtct tttgcgtggt aacagcgcgc
    47461 tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat tttctcgagc
    47521 tgatccgcac cggcaggccg gacgaagaaa gctatctgca cgcggcggtg gaactggcgg
    47581 aagcgcgcgg tttgcccgtc gtggcgacta acgatgtgcg ctttatcgac agcagcgact
    47641 ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac gatcctaaac
    47701 gccctcgtaa ctattcgccg cagcaatata tgcgtagcga agaggagatg tgtgagctgt
    47761 ttgccgacat ccccgaagcc cttgccaaca ccgttgagat tgccaaacgt tgtaacgtaa
    47821 ccgtgcgtct gggtgaatac ttcctgccgc aattcccgac cggggacatg agtaccgaag
    47881 attatctggt caagcgtgca aaagaaggcc tggaagagcg tctggcattt ttattccctg
    47941 acgaggaaga acgtcttaag cgtcgtccgg agtatgacga acgtctggat actgaacttc
    48001 aggttatcaa ccagatgggc ttcccgggtt acttcctcat cgttatggaa tttatccagt
    48061 ggtcgaaaga taacggtgta ccggtagggc caggccgtgg ttctggtgca gggtcactgg
    48121 tggcctacgc gttaaaaatc accgatctcg atccgctgga atttgacctg ctgttcgaac
    48181 gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc tgtatggaga
    48241 aacgtgacca ggttatcgaa catgtggcgg acatgtacgg tcgcgatgcg gtatcgcaga
    48301 ttatcacctt cggtacgatg gcggcgaaag cggtgatccg cgacgtaggc cgtgtattgg
    48361 ggcatccgta cggatttgtc gatcgtatct cgaaactgat cccgcccgat ccgggtatga
    48421 cgctggcgaa agcgtttgaa gccgagccac agctgccgga aatctacgaa gcggatgaag
    48481 aagttaaggc gctgatcgac atggcgcgca aactggaagg ggtcacccgt aacgccggta
    48541 agcacgccgg tggggtggtt atcgcgccga ccaaaattac tgattttgcg ccgctttatt
    48601 gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt gaatacgccg
    48661 ggctggtgaa gttcgacttc ctcggtttgc gtacgctcac catcatcaac tgggcgctgg
    48721 agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc gccgcgatcc
    48781 cgctggatga caagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg gcggtattcc
    48841 agcttgaatc gcgcggcatg aaggacctga tcaagcgtct gcaacctgac tgcttcgaag
    48901 atatgatcgc actggtggca ctgttccgcc ccggcccgtt gcaatcaggg atggtggata
    48961 actttatcga ccgtaaacat ggtcgcgaag agatctccta tccggacgtg cagtggcagc
    49021 atgaaagcct gaaaccggta ctggagccaa cctacggcat catcctgtat caggaacagg
    49081 tcatgcagat tgcccaggtg ctttctggtt ataccctcgg tggcgcggat atgctgcgtc
    49141 gtgcgatggg taagaaaaag ccggaagaga tggctaagca gcgttctgta tttgctgaag
    49201 gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa aatcttcgac ctggtggaga
    49261 aattcgcggg ttacggattt aacaaatcgc actctgcggc ctatgctttg gtgtcatatc
    49321 aaacgttatg gctgaaagcg cactatccgg cggagtttat ggcggcggta atgaccgccg
    49381 atatggacaa caccgagaag gtggtgggcc tggtggatga gtgctggcgg atggggctga
    49441 aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac gacgacggcg
    49501 aaatcgtgta tggtattggc gcgatcaaag gggtaggtga aggcccgatt gaggccatca
    49561 tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc gcccgtaccg
    49621 acaccaaaaa gttaaaccgg cgggtgctgg aaaaactgat catgtccggg gcgtttgacc
    49681 gtcttgggcc acaccgcgcg gcgctgatga actcgctggg cgatgcgtta aaagcggcag
    49741 atcaacacgc gaaagcggaa gctatcggtc aggccgatat gttcggcgtg ctggccgaag
    49801 agccggaaca aattgaacaa tcctacgcca gctgccaacc gtggccggag caggtggtat
    49861 tagatgggga acgtgaaacg ttaggtctgt acctgacggg acaccctatc aaccagtatt
    49921 taaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac ccgacagaac
    49981 gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg gtcaccaagc
    50041 gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg gaagtgatgt
    50101 tgtttactga cgccctggat aaataccagc aattgctgga aaaagaccgc atacttatcg
    50161 tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc gctcgcgaag
    50221 tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc tcgctgacgg
    50281 acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa ccccaccgct
    50341 ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg cgattgcgtt
    50401 ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc cgtggcctca
    50461 ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg agtctgaatt
    50521 tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct ctgaccgcgg
    50581 ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat cgtctgcgtg
    50641 aaaaaagcgt agaactgacg cgtaaaatct tcgccgatct cggtgcatgg cagattgcgc
    50701 aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg gcatttgatg
    50761 aatttgacga actggctgga gaccgcgcgt atgcagacga taaagctatc gtcggtggta
    50821 tcgcccgtct cgatggtcgt ccggtgatga tcattggtca tcaaaaaggt cgtgaaacca
    50881 aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc aaagcactgc
    50941 gtctgatgca aatggctgaa cgttttaaga tgcctatcat cacctttatc gacaccccgg
    51001 gggcttatcc gggcgtgggc gcagaagaac gtggtcagtc tgaagccatt gcacgcaacc
    51061 tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac ggttatcggt gaaggtggtt
    51121 ctggtggtgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa tacagcacct
    51181 attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgct gacaaagcgc
    51241 cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct gaaagagctg aaactgatcg
    51301 actccatcat cccggaaccg ctgggcggtg ctcaccgtaa cccggaagcg atggcggcgt
    51361 ctctgaaagc gcagctgctg gcggatctgg ccgatctcga cgtgttaagc actgaagatt
    51421 taaaaaatcg tcgttatcag cgcctgatga gctacggcta cgcgtaatcg gcaaatgttc
    51481 tgaaaagggt cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa
    51541 ggaaggattt tccaggagga acacatgaac atcattgcca ttatgggact gcatggcgtc
    51601 ttttataaag atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag
    51661 attatctggc cacaaaacag cgttgatttg ctgaagttta tcgaacataa cccacgaatt
    51721 tgcggcgtga tttttgactg ggatgagtac agtctcgatt tatgtagcga tatcaatcaa
    51781 cttaatgaat atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc
    51841 gtgcaggata tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat
    51901 atcgccattc gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc
    51961 acgaaagcct tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat
    52021 atgggcggca ccgcatatca aaaaagcccg gttggctgtc tgttttatga ttttttcggc
    52081 gggaataccc tcaaggcgga cgtctctatt tcggtcaccg agcttggttc gttgctcgac
    52141 cacaccgggc cacacctgga agcggaagag tacatcgcgc ggacgtttgg cgcggaacag
    52201 agttatatcg ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg
    52261 ccatccggca gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg
    52321 atgatgaacg atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctg
    52381 ggtgggatcc cgcgcggtga atttactcgc gacagcatcg aagagaaagt cgctgccacc
    52441 acgcaggcac aatggccggt tcatgcggtg atcaccaact ccacctatga tggcttgctc
    52501 tacaacaccg actggatcaa acagacgctg gatgtcccgt cgattcactt cgattctgcc
    52561 tgggtgccgt acacccattt tcatccgatc taccagggta aaagtggtat gagcggcgag
    52621 cgtgttgcag gaaaagtgat cttcgaaacg cagtcgaccc acaaaatgct ggcggcgtta
    52681 tcgcaggcgt cgctgatcca cattaaaggt gagtatgacg aagaggcgtt taacgaagcc
    52741 tttatgatgc ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg
    52801 gcggcgatgc tgcgtggtaa tccgggcaaa cggctgatta accgttcagt agaacgagct
    52861 ctgcattttc gcaaagaggt ccagcggctg cgggaagagt ctgacggctg gttttttgat
    52921 atctggcaac cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc tggcgaacag
    52981 tggcacggct ttagcgatgc ggatgccaat cacatgtttc tcgatccggt taaagtcact
    53041 attttgacac cggggatgga cgagcagggc aatatgagcg aggaggggat cccggcggcg
    53101 ctggtggcaa aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac
    53161 ctgctgtttc tctttagtat tggcatcgat aaaaccaaag caatgggatt attgcgtggg
    53221 ttgacggaat ttaagcgctc ttacgatctc aacctgcgga tcaaaaatat gctgcccgat
    53281 ctctatgcag aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg
    53341 atccataagc tgattcgtaa acacgatctt cccggtttga tgttgcgggc atttgatacc
    53401 ttgccggaga tgatcatgac gccacatcag gcatggcaga gacagattaa aggcgaagta
    53461 gaaaccattg cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat
    53521 ccacccggtg taccgctgct gatgccggga gaaatgctta ccgaagagag ccgcacggta
    53581 ctcgattttc tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat
    53641 attcacggcg cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg
    53701 ggataacttg ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg
    53761 ttatgctggg tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcgatga
    53821 gcaaagcgtt ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag
    53881 cgcgcgactc gtggaaaggg gatttggcgc ttaagggaca atatgtgatt gagcttttct
    53941 catttccgtt cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg
    54001 cttttagcgt tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt
    54061 gtgaagccat ccgtgtcgat ccctacacgc aaaaacgctt cacgttcttt aacgatccgg
    54121 acgggctgcc attggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta
    54181 acgtgccggg cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa
    54241 cttctctcct cacgccagat tctggtggcc tttagcggcg gccttgattc tactgtctta
    54301 ctgcatcaac tggtgcagtg gcggacggaa aatccgggcg tcactctgcg cgctatccat
    54361 gtgcatcacg gtttaagcgc caatgctgat gcctgggtga aacattgtga aaacatctgc
    54421 caacagtggc aggtgccgct ggtggtcgaa cgtgtgcagc ttgcgcaaga ggggctaggt
    54481 attgaggccc aggcgcggca ggcacgttat caggcatttg cccgcacctt gttgcccggt
    54541 gaagtgctgg ttacagcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta
    54601 aaacgcggta gcggccctgc cgggctttcg gctatggcgg aagtttcgga gtttgccgga
    54661 acacggctta tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcgtta
    54721 gcgcatggtt tacgctggat tgaagacgaa agtaatcagg atgactcata cgatcgtaac
    54781 tttctgcgcc tgcgcgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca
    54841 acagcccgca gcgccgcact ttgcgctgaa caagagagcc tgttagatga actgctggcg
    54901 gatgatttag cgcactgcca aacgcaacag ggaacgttaa agattgcgcc aatggtggca
    54961 atgagcgatg cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg
    55021 atgccttccc gcgacgcgtt ggtgaggatc tggcaggaag tggcgctggc gcgagaagat
    55081 gcttccccct gtttacgttt gggtgcgttt gaaatccgcc gctatcaatc gcaactgtgg
    55141 tggattaaat ccgtcactgg gcaaagcgaa accattctgc cgtggcagac gtggctacaa
    55201 ccgctggaat taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc
    55261 cctccgcgtg cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt
    55321 gtcgggcgca acggcggacg taagctgaag aaaatctggc aagagttagg cgttccgccg
    55381 tggctacgtg acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg
    55441 gtatttgtga cgcaagaagg tgtggctgta ggtgagaatg gcgtcagttt tgtctggcag
    55501 aaaacgctta gtcaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt
    55561 caatcaggac tcgctcacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt
    55621 atccagacgc agctcgcggg ttgcaccagc ggcctcgacg accagatact ccacattttt
    55681 gcgggagact aaatcgctgg ccttcgcctg caatttttcg ccatctttca gctcaagtgt
    55741 cagcattaaa tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg
    55801 ttgatacgta tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg
    55861 ctttttccct gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca
    55921 aaacgcagcc cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc
    55981 gcttgcgtat taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac
    56041 cgtggatgca agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca
    56101 ctcatttctg caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt
    56161 cgaaaggagt tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc
    56221 cgatctcgac cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc atccttcaga
    56281 aacccaggag cgcatgatgc tgcgcctgct ggcgtggcta aaatatgccg atgaacgtct
    56341 gcaatttacc cgtggtttgt gtgccgatga tgagccggaa gcgtggctgc gtaacgatca
    56401 tttgggtatt gatttgtgga ttgagctggg gctgccggat gagcgacgga ttaagaaagc
    56461 ctgcacccag gccgcagaag tggcgctgtt tgcctataat agtcgggcgg cgcaaatctg
    56521 gtggcagcaa aatcagagca aatgtgcgca gtttgccaat ctttccgtct ggtatctgga
    56581 cgacgagcag ctggcgaaag tgagcgcctt tgctgatcgt actatgacgc tgcaggcaac
    56641 gattcaggat ggtgttatct ggttatcgga tgataagaat aatctggaag tgaacttaac
    56701 cgtctggcaa caaccttcat gattgtgatt tcccgacatg ttgctatccc tgatggcgag
    56761 tttgagatca ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca
    56821 acggctattc atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag
    56881 cgtctgctcg ccgccagcca tcatttgatc agcagtgacg gagtgattgt cattaaggca
    56941 caggaatacc gcagtcagga actgaaccgc gaagcggcgc tggcccggct ggtggcagtg
    57001 attaaagatt taacaacaga acaaaaagcc cgacgaccta cgcggcccac ccgtgcatca
    57061 aaagagcgca ggctggcatc gaaagcacaa aaatcaagcg tgaaggcgat gcgcggcaaa
    57121 gtgcgcagcg gtcgggaata aaaagaagga atggatggtg aaaaaagcga tagtgacagc
    57181 gatggctgta atcagcctct ttactctgat gggatgtaat aatcgggccg aagtcgatac
    57241 gctttctccg gcgcaggctg ccgaactgaa accgatgccg caaagttggc gtggcgtgct
    57301 gccgtgtgct gattgcgaag gaatcgaaac ctctctgttc ctcgaaaaag acggaacctg
    57361 ggtgatgaat gagcgttatc tgggggctcg tgaagaacct tcctccttcg cttcctacgg
    57421 tacatgggcg cgaaccgctg acaagctggt attaaccgat agcaaaggtg aaaagtcata
    57481 ttatcgcgcg aaaggagatg cgctggagat gctcgatcgt gaaggtaatc cgattgaatc
    57541 gcagttcaac tacacgctgg aaccggcaca atccagctta cccatgacgc cgatgaccct
    57601 gcggggcatg tatttttata tggctgatgc ggcgaccttc actgattgcg cgaccggaaa
    57661 acgtttcatg gtggcgaata acgcagagct ggagcgtggc tacctggctg cgcgcggtaa
    57721 cagtgaaaaa ccggtgttac tgtcagtaga aggtcacttt acgcttgagg ctaatccgga
    57781 taccggtgcg ccgaccaaag tattagcgcc cgatacggca ggtaaatttt acccaaacca
    57841 ggattgcagt agtttggggc tgtaacccgt cttgagacag aaacaaatga aatattgcct
    57901 catgcgctgc gcttatcagt cctaaagcat acaatgcaat attttgaatt agcacaattt
    57961 tgtaggccgg ataaggcgtt cacgccgcat ccggcatttt gcgcacgagg ctggctttta
    58021 tcactgagtc agcccaacaa agcggcctgc cttgatataa atcccaggct tcatgtgccc
    58081 aacatactgt aactgcgttt ctggcttaaa cgcagagata tcgccagtac gcatatgcat
    58141 cagatcggca gggctaatcc agcccgattg cgccagtgta agcggatgcc cggctttggc
    58201 aaatgcatca gtgacaaatt ccgaacaaaa ccacgacttt ttgtctcctt cgcccacact
    58261 gcttaactgc gctttcgcca ggccgctgac gcactgttga cgaaaatcct cggagaacgg
    58321 attcagtgag cacatctggc gagttaccat aaagggaata aattcgacaa tgccgcgata
    58381 gttgtaaccg ctatctttaa ttttgttggc gaacgcggtg atttctgtgg cttgttgcgg
    58441 ggtaagatcc gggactcgta agacgaaaag cttatcgcta tgcttcatcg cttttttgag
    58501 ggaaacaatc tggacgccag cgcctgtcgc ttctgcaacg ttattttcac ccagatagat
    58561 tgcaacgtga ctcacagagg aagtgctgaa gacgcggatt ccaaatgagg ttaccccaag
    58621 gcttgaggag aacagcaaat cgccgggttt gagatctggt tccgttattt cttttattga
    58681 ttgttcggtg aaagagcttt gatgctggaa tttaacagcc catgttttcg cctcggcatt
    58741 taccactgtg gctgatgagt ctggctggct gatatcaacg gtacaggccg aaagtaaaag
    58801 aaagcagggg agaagcaggc ggcagtacgc ctttggttta tccattttat acaatccatg
    58861 taaaaaaggg ccccgaaatt caggaccctt tctggcatca gcctttaatc tgtttcacca
    58921 gataatcgac gatgtcacca gtcttaatta actgtttctc gccgttacga cgatatttat
    58981 attcgatatc atcgttgtcg aggttacggt cgcccagcac aatagtgtgc ggaataccga
    59041 tcagttccat atcagcaaac atcacgcccg gacgctcttt gcggtcatcc agcagcactt
    59101 cgataccttg tgcgcgcagt tcgctgtaca gtttctcagc aagttcctgt acgcggaagg
    59161 atttgtgcat gttcattggc agaatcgcca cctggaacgg cgcgatagcg tcaggccata
    59221 cgatgccgcg ttcgtcgtag ttctgctcaa tcgccgcagc taccacacgc gttacgccga
    59281 taccgtagca acccatcgtc aggatttggt tacggccatc ttcaccctgt acggaggctt
    59341 tcagtgcttc ggagtacttg gtacccagct ggaagatgtg accgacttcg ataccacgtt
    59401 tgatcagcag cgtaccctga ccatccgggc ttggatcgcc agccaccacg ttgcggatat
    59461 cagcaatttc cggggtagcg acatcgcgat cccagttgat accgaagtag tgtttaccat
    59521 cgatgttagc accagcagcg aaatcactca tcgccgcaac ggtacggtca atcaccaccg
    59581 gaatcggcat gtttaccgga cccagtgaac ccggaccggc tttaaccacg gcacgaattt
    59641 cttcttcggt cgcaaaagtc agcgggctgg caacctgcgg cagtttttct gctttaactt
    59701 cgttcagctc gtggtcaccg cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa
    59761 cggctttaac cagcagagtc ttaaccgttt tctcaatcgg cagattgaac tgttcaacca
    59821 gttccgcgat ggtcttcgcg ttcggcgtat caaccagcgt catttcctgg gtagcagcag
    59881 cgcgcggttc tttcggcgcg atagcttctg ccagttcaat gttcgctgca tagtcagaag
    59941 tatcggagaa gaccacatcg tcttctccgc tctgcgccag cacctgaaat tcgtgagagg
    60001 cgctaccgcc gatagaaccg gtatcggcct gtacggcgcg gaaatccagc cccatgcggc
    60061 tgaagatttt gctgtaggcc gcatacattg catcgtaggt ttcctgcagg gattcctgag
    60121 aagtatggaa agagtaagca tctttcatca ggaattcgcg ggaacgcatg acgccgaaac
    60181 gcggacgcac ttcgtcgcgg aacttggtct ggatctgata gaagttcagc ggcagctgtt
    60241 tgtaagagct cagctcgtta cgaatcaggt ctgtaatcac ttcttcatga gttgggccga
    60301 gtacgaacgg acggtcacca cggtcagcaa tacgcagcaa ttccgggcca tactgttccc
    60361 aacgaccact ctcttgccac agttcagatg gctgaaccac cggcattaac acctcgatcg
    60421 caccggcgtt gttcatctct tcacgcacga tgttttcgac ttttttcaga acgcgcacgc
    60481 cggtcggcag ccaggtatat aacccggagg ccagcttgcg gatcatcccg gcgcgcagca
    60541 tcagctgatg gctgatcacc tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt
    60601 attggctagt acgcatgttg ttacggttcc agttggaagg tagaacaggc tcaaggcgag
    60661 cctgggacaa aaaaagtgat ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc
    60721 gtgaatttaa cgcggttcca gcgcaaagac ttcaaaacct gcgtcggtga cgcgccagcg
    60781 aacgttaaaa tcatgcagcc agacggcata ggttttgccc gtttcctcac ctttacgata
    60841 ggccgggcgc gggtcctgcg ccagtacttc gcggataaac agcgttaact gcggataacg
    60901 cttcttcagc gtcaaaagct gctttttgac ctctgcggta aaacacaccg ccatttctgc
    60961 cgctggcgca ctttgcgcat agctggcact ggcatcggga agcgattcag caaaggggag
    61021 atacggtttg atatccacta ccggcgtacc atcgaccaga tccagactgc cgagcttcag
    61081 aatcacgcta tctttatggc aaacaacctc tttcagctct accagcgaca taccaatcgg
    61141 gttagggcga aaagtggagc gcgtagcgaa aacacccatt ctggcattac cgccgagacg
    61201 aggcggacgc acagtcggac gccagccgcc ttccatcgtt tgatgaaaga cgaaaaggat
    61261 ccataaatgg ctgaatgctt ccaggccgcg aacggcgtcg gcctggttgt agggagcaat
    61321 gagatgtagt tcgccgttgg cgctttttac cagacctggc tggcgcggaa cggcgaattt
    61381 ttctttatag ggcgagcgaa taacgcctat ttgctcgaac tggaaactgc tcatttcgcc
    61441 gtgatgttaa gcgcagaacc gatacataca gcctgacgat agcaacctgg cgtaccgctg
    61501 gtgacttcgc agctatgcag taataccgca ttggctttca ttttcgaggc gttgatttgc
    61561 atccgcttac gtgcggttgg aatgctcggc ggagagtcct gattagaggc ctggcaagag
    61621 tcgccactga cttcaccgag atcgcggaac ggtttgccga ctaattcttc tgcattggta
    61681 taaattcgga ccggcgtggc gcgcggcgct ttcggttttg caggctccgc tttcggctgg
    61741 ggtgcagtgc tttgaacggg ttcgacaggg gatctgctta acatggaaca gccgcttagc
    61801 atgagtgcta ctaaacagat cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa
    61861 acgtcaatat tgaatcaggc gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa
    61921 tgatattaca aattgtggaa acagcctaaa tattaccagc ctttaacagc gccgccgtta
    61981 aacactttgt ttgctgcttc gtaaacttcg tcagactgat aagcctgaac gaatttcttc
    62041 acgttttcgg cgtctttgtt gtcttcacgc gttacgatca ggtttacgta cggggactct
    62101 ttatcttcga caaagatacc gtctttcgct ggagtcaggc caatctggct ggcataggtg
    62161 gtattgataa ctgccagagc gatttgcgcg tcgtccagag agcgcggcag ctgcggtgct
    62221 tccagttcaa caattttcag attttttggg ttctcaacaa catcaagaac ggtcggcagc
    62281 aggccaacgc catctttcag tttgatcaag cccacttttt gcagcagcag cagtgaacga
    62341 ccaaggttag ttgggtcgtt tggcacggca acctgcgaac catcctgcag ttcatccagt
    62401 gatttgattt tcttggagta accagcaatc ggataaacaa aagtgttgcc tactgcgacc
    62461 agtttgtagc cacgatcttt cagttgctga tcaaggtacg gtttatgctg gaaggcgttg
    62521 gcgtcgatat cgcctttgct caatgcttcg tttggcagaa catagtcgtt gaaggttacc
    62581 agctcaacgt ccaggccata tttgtctttc gcaactttct gcgcgacttc tgcaacctgc
    62641 tgttcggcac caacaatcac gccgacttta atgtggtttg gatctttttc atcctgaccg
    62701 cagcctacca gtgccagtga tccaatcagg gctcccactg ccgcaaaggt tttgaatttg
    62761 aacgccatac cttattcctt cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg
    62821 acagcccgga cgatgcggtc gcctgcgaac tgaattaaat aaaccagaat gaccagcaat
    62881 accagtaccg tattcatcac cgtcgcgttg tagccgatgt agccatactg atagccaatc
    62941 tgacctaaac caccggcacc gactgcacca cccatcgcgg aataaccaac cagggtaatc
    63001 agggtgatag ttgccgcatt caccagaccc ggcaacgctt ccggtaacag cactttacgg
    63061 acgatctgca ttggcgtggc ccccattgcg cgggaagctt caattaaccc ggttgggatc
    63121 tccagcagag cgttctcgac catacgggca ataaacggtg ctgcaccaac ggttaacgga
    63181 acaatcgctg cctgcaatcc aatcgatgta ccgacaataa cgcgggtaaa cggaatcatc
    63241 cataccagca agataatgaa cgggatggaa cggaaaatgt tcacaatcgc agaaatggta
    63301 cgatacaact tcgcgttagc aataatttgc cccggacgcg tgacataaag cagaacgcca
    63361 accggcagac caatcacaaa gccaaaaaaa ccggatacga aggtcattgc cagcgtttcc
    63421 catacgccac gaaccagcag ccacatcatc ggctcagaca taacccagta cctctacttt
    63481 tacatggtgt tcctgcagcc aggcaatggc ggcttgcgta tcttgttgtg tgccgtgcat
    63541 ttcagtcagc atgatgccga acttaacgcc accggcgtaa tccatctgcg cgctaataat
    63601 gttgttgttg acgttgaaac gacgcgcggt ttcagaaagc agtggggcat cgaccgattg
    63661 accggtaaac tccagacgca gcatcggcac gcagtcagta aatggctccg cttgcagacg
    63721 ttcctggtaa tcttccggga tatccagatg cagggtcgac tgaataaact tctgcgccag
    63781 cggcgttttc ggatgcgaga acacttcact taccgtgtcc tgctcgatca gttcgccatt
    63841 gctgatgacc gccacgcaat cacaaatgcg cttcacaacg tccatttcgt gagtgatcaa
    63901 cagaatcgtc aaacccagac ggcggttgat gtctttcagc agttcgagaa tagaacgtgt
    63961 cgttgccggg tccagcgcgc tggtggcttc atcacacagc aatactttgg gattgctggc
    64021 taatgcacgg gcaatcgcca cacgttgttt ctgcccaccg gaaagattcg acgggtagct
    64081 atcatgctta tcgccaagac caaccaatga cagcaattcc gtcacgcgac gtttgatctc
    64141 gtctttcggt gtgttgtcca gctccagcgg cagagccacg ttgccaaaaa cagtacgcga
    64201 agagagcagg ttaaaatgct ggaaaatcat accaatctgg cgacgagctt tggtcaactc
    64261 ggattctgac agcgtggtca gttcctggcc atcgaccagc acgctaccct cggttgggcg
    64321 ctccagcagg tttacacaac gtataagcgt actcttaccc gcgcctgagg caccgataac
    64381 gccataaatt tgtccagctg gcacatgcag gctgacgttg ttcaacgcct ggatggtgcg
    64441 ggtgccctgg tggaacactt tggtgatatt cgaaagtttt atcattgatt atttattatc
    64501 gtcattaagt tagtcgtggc atttcgaatg cctggaacgg gcaacgccgt caatgaaatg
    64561 gatgttaagg catccagacg tctaaatcaa tcaggtttat gcgaagagca ctttcttgca
    64621 ggtcgaaaca tgcgatacta gcgtcacatg ccttattaag gagctataaa aggtggcgaa
    64681 gagcgtaccc gcaatttttc ttgaccgtga tggcaccatt aatgtcgatc acggttatgt
    64741 ccatgagatc gacaactttg aatttatcga cggtgttatt gacgccatgc gcgagctaaa
    64801 aaaaatgggc tttgcgctgg tggtagtaac caaccagtct ggcattgctc gcggtaaatt
    64861 tactgaagca cagtttgaaa cgctgaccga gtggatggac tggtcgctgg cggaccgcga
    64921 tgtcgatctg gatggtatct attattgccc gcatcatccg cagggtagtg ttgaagagtt
    64981 tcgccaggtc tgcgactgcc gcaaaccaca tccggggatg tttttgtcag cacgcgatta
    65041 tttgcatatt gatatggccg cttcttatat ggtgggcgat aaattagaag atatgcaggc
    65101 agcggctgcg gcgagcgtgg gaacaaaagt gctggtgcgt acgggtaaac ctgttacgcc
    65161 tgaagcagaa aacgcggcgg attgggtgtt aaatagcctg gcagacctgc cgcaagcgat
    65221 aaaaaagcag caaaaaccgg cgtaatgatt aaaagatgag cggttgaaat aaaaatgcat
    65281 ttttccgctt gtcttcctga gacgactccc tataatgcgc ctccatcgac acggcggatg
    65341 tgaatcactt cacacaaaca gccggttcgg ttgaagagaa aaatcctgaa attcagggtt
    65401 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaagccgcgt
    65461 cgcaactgct ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc
    65521 ttgacgtcgc aagacgaaaa atgaatacca agtctca
//
LOCUS       DAZFRN010000005        65541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFRN010000005 DAZFRN010000000
VERSION     DAZFRN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.005"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..897)
                     /locus_tag="ACHKUB_000283"
     CDS             complement(<1..897)
                     /locus_tag="ACHKUB_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO6653366.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAG"
     gene            1233..2435
                     /gene="nupC"
                     /locus_tag="ACHKUB_000284"
     CDS             1233..2435
                     /gene="nupC"
                     /locus_tag="ACHKUB_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO6653367.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2485..4674)
                     /gene="pdeA"
                     /locus_tag="ACHKUB_000285"
     CDS             complement(2485..4674)
                     /gene="pdeA"
                     /locus_tag="ACHKUB_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO6653368.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(4883..4958)
                     /locus_tag="ACHKUB_000286"
     tRNA            complement(4883..4958)
                     /locus_tag="ACHKUB_000286"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4923..4925),aa:Ala,seq:ggc)
     gene            complement(4998..5073)
                     /locus_tag="ACHKUB_000287"
     tRNA            complement(4998..5073)
                     /locus_tag="ACHKUB_000287"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5038..5040),aa:Ala,seq:ggc)
     gene            5294..5653
                     /gene="yfeC"
                     /locus_tag="ACHKUB_000288"
     CDS             5294..5653
                     /gene="yfeC"
                     /locus_tag="ACHKUB_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6653369.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            5655..6047
                     /gene="yfeD"
                     /locus_tag="ACHKUB_000289"
     CDS             5655..6047
                     /gene="yfeD"
                     /locus_tag="ACHKUB_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6653370.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6099..7514)
                     /gene="gltX"
                     /locus_tag="ACHKUB_000290"
     CDS             complement(6099..7514)
                     /gene="gltX"
                     /locus_tag="ACHKUB_000290"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO6653371.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            7773..7848
                     /locus_tag="ACHKUB_000291"
     tRNA            7773..7848
                     /locus_tag="ACHKUB_000291"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7806..7808,aa:Val,seq:tac)
     gene            7893..7968
                     /locus_tag="ACHKUB_000292"
     tRNA            7893..7968
                     /locus_tag="ACHKUB_000292"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7926..7928,aa:Val,seq:tac)
     gene            8015..8090
                     /locus_tag="ACHKUB_000293"
     tRNA            8015..8090
                     /locus_tag="ACHKUB_000293"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8048..8050,aa:Val,seq:tac)
     gene            8095..8170
                     /locus_tag="ACHKUB_000294"
     tRNA            8095..8170
                     /locus_tag="ACHKUB_000294"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8128..8130,aa:Lys,seq:ttt)
     gene            8290..8631
                     /locus_tag="ACHKUB_000295"
     CDS             8290..8631
                     /locus_tag="ACHKUB_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO6653372.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQCLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(8622..9548)
                     /gene="yfeR"
                     /locus_tag="ACHKUB_000296"
     CDS             complement(8622..9548)
                     /gene="yfeR"
                     /locus_tag="ACHKUB_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6653373.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            9638..10636
                     /gene="yfeH"
                     /locus_tag="ACHKUB_000297"
     CDS             9638..10636
                     /gene="yfeH"
                     /locus_tag="ACHKUB_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO6653374.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(10633..10851)
                     /gene="ypeB"
                     /locus_tag="ACHKUB_000298"
     CDS             complement(10633..10851)
                     /gene="ypeB"
                     /locus_tag="ACHKUB_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO6653375.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(10853..12868)
                     /gene="ligA"
                     /locus_tag="ACHKUB_000299"
     CDS             complement(10853..12868)
                     /gene="ligA"
                     /locus_tag="ACHKUB_000299"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO6653376.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(12939..13925)
                     /gene="zipA"
                     /locus_tag="ACHKUB_000300"
     CDS             complement(12939..13925)
                     /gene="zipA"
                     /locus_tag="ACHKUB_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO6653377.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            14155..14916
                     /gene="cysZ"
                     /locus_tag="ACHKUB_000301"
     CDS             14155..14916
                     /gene="cysZ"
                     /locus_tag="ACHKUB_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO6653378.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            15101..16072
                     /gene="cysK"
                     /locus_tag="ACHKUB_000302"
     CDS             15101..16072
                     /gene="cysK"
                     /locus_tag="ACHKUB_000302"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO6653379.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            16456..16713
                     /gene="ptsH"
                     /locus_tag="ACHKUB_000303"
     CDS             16456..16713
                     /gene="ptsH"
                     /locus_tag="ACHKUB_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO6653380.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            16758..18485
                     /gene="ptsI"
                     /locus_tag="ACHKUB_000304"
     CDS             16758..18485
                     /gene="ptsI"
                     /locus_tag="ACHKUB_000304"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO6653381.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            18526..19035
                     /gene="crr"
                     /locus_tag="ACHKUB_000305"
     CDS             18526..19035
                     /gene="crr"
                     /locus_tag="ACHKUB_000305"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO6653382.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(19077..19928)
                     /gene="pdxK"
                     /locus_tag="ACHKUB_000306"
                     /gene_synonym="thiJ"
     CDS             complement(19077..19928)
                     /gene="pdxK"
                     /locus_tag="ACHKUB_000306"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO6653383.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            20033..20401
                     /gene="yfeK"
                     /locus_tag="ACHKUB_000307"
     CDS             20033..20401
                     /gene="yfeK"
                     /locus_tag="ACHKUB_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO6653384.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(20404..21315)
                     /gene="cysM"
                     /locus_tag="ACHKUB_000308"
     CDS             complement(20404..21315)
                     /gene="cysM"
                     /locus_tag="ACHKUB_000308"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO6653385.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(21449..22546)
                     /gene="cysA"
                     /locus_tag="ACHKUB_000309"
     CDS             complement(21449..22546)
                     /gene="cysA"
                     /locus_tag="ACHKUB_000309"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO6653386.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(22536..23411)
                     /gene="cysW"
                     /locus_tag="ACHKUB_000310"
     CDS             complement(22536..23411)
                     /gene="cysW"
                     /locus_tag="ACHKUB_000310"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO6653387.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(23411..24244)
                     /gene="cysT"
                     /locus_tag="ACHKUB_000311"
     CDS             complement(23411..24244)
                     /gene="cysT"
                     /locus_tag="ACHKUB_000311"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO6653388.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(24244..25260)
                     /gene="cysP"
                     /locus_tag="ACHKUB_000312"
     CDS             complement(24244..25260)
                     /gene="cysP"
                     /locus_tag="ACHKUB_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO6653389.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(25418..26209)
                     /gene="ucpA"
                     /locus_tag="ACHKUB_000313"
     CDS             complement(25418..26209)
                     /gene="ucpA"
                     /locus_tag="ACHKUB_000313"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO6653390.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(26338..27195)
                     /gene="murR"
                     /locus_tag="ACHKUB_000314"
     CDS             complement(26338..27195)
                     /gene="murR"
                     /locus_tag="ACHKUB_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO6653391.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            27359..28255
                     /gene="murQ"
                     /locus_tag="ACHKUB_000315"
     CDS             27359..28255
                     /gene="murQ"
                     /locus_tag="ACHKUB_000315"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO6653392.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            28259..29683
                     /gene="murP"
                     /locus_tag="ACHKUB_000316"
     CDS             28259..29683
                     /gene="murP"
                     /locus_tag="ACHKUB_000316"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO6653393.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            29688..30992
                     /gene="pbp4b"
                     /locus_tag="ACHKUB_000317"
     CDS             29688..30992
                     /gene="pbp4b"
                     /locus_tag="ACHKUB_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO6653394.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(31050..31949)
                     /gene="yfeX"
                     /locus_tag="ACHKUB_000318"
     CDS             complement(31050..31949)
                     /gene="yfeX"
                     /locus_tag="ACHKUB_000318"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO6653395.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVGAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(32045..32620)
                     /gene="yfeY"
                     /locus_tag="ACHKUB_000319"
     CDS             complement(32045..32620)
                     /gene="yfeY"
                     /locus_tag="ACHKUB_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO6653396.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(32681..33130)
                     /gene="yfeZ"
                     /locus_tag="ACHKUB_000320"
     CDS             complement(32681..33130)
                     /gene="yfeZ"
                     /locus_tag="ACHKUB_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO6653397.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(33117..33542)
                     /gene="ypeA"
                     /locus_tag="ACHKUB_000321"
     CDS             complement(33117..33542)
                     /gene="ypeA"
                     /locus_tag="ACHKUB_000321"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO6653398.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            33756..34625
                     /gene="amiA"
                     /locus_tag="ACHKUB_000322"
     CDS             33756..34625
                     /gene="amiA"
                     /locus_tag="ACHKUB_000322"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO6653399.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            34629..35528
                     /gene="hemF"
                     /locus_tag="ACHKUB_000323"
     CDS             34629..35528
                     /gene="hemF"
                     /locus_tag="ACHKUB_000323"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO6653400.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(35534..36586)
                     /gene="eutR"
                     /locus_tag="ACHKUB_000324"
     CDS             complement(35534..36586)
                     /gene="eutR"
                     /locus_tag="ACHKUB_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO6653401.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHKPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(36632..37132)
                     /gene="eutK"
                     /locus_tag="ACHKUB_000325"
     CDS             complement(36632..37132)
                     /gene="eutK"
                     /locus_tag="ACHKUB_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO6653402.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(37145..37804)
                     /gene="eutL"
                     /locus_tag="ACHKUB_000326"
     CDS             complement(37145..37804)
                     /gene="eutL"
                     /locus_tag="ACHKUB_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO6653403.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(37814..38701)
                     /gene="eutC"
                     /locus_tag="ACHKUB_000327"
     CDS             complement(37814..38701)
                     /gene="eutC"
                     /locus_tag="ACHKUB_000327"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO6653404.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(38722..40083)
                     /gene="eutB"
                     /locus_tag="ACHKUB_000328"
     CDS             complement(38722..40083)
                     /gene="eutB"
                     /locus_tag="ACHKUB_000328"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO6653405.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(40095..41498)
                     /gene="eutA"
                     /locus_tag="ACHKUB_000329"
     CDS             complement(40095..41498)
                     /gene="eutA"
                     /locus_tag="ACHKUB_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO6653406.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(41495..42721)
                     /gene="eutH"
                     /locus_tag="ACHKUB_000330"
     CDS             complement(41495..42721)
                     /gene="eutH"
                     /locus_tag="ACHKUB_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO6653407.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(42838..44025)
                     /gene="eutG"
                     /locus_tag="ACHKUB_000331"
     CDS             complement(42838..44025)
                     /gene="eutG"
                     /locus_tag="ACHKUB_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO6653408.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDHD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(44015..44851)
                     /gene="eutJ"
                     /locus_tag="ACHKUB_000332"
     CDS             complement(44015..44851)
                     /gene="eutJ"
                     /locus_tag="ACHKUB_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO6653409.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(44862..46265)
                     /gene="eutE"
                     /locus_tag="ACHKUB_000333"
     CDS             complement(44862..46265)
                     /gene="eutE"
                     /locus_tag="ACHKUB_000333"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO6653410.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(46277..46564)
                     /gene="eutN"
                     /locus_tag="ACHKUB_000334"
     CDS             complement(46277..46564)
                     /gene="eutN"
                     /locus_tag="ACHKUB_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO6653411.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(46671..46964)
                     /gene="eutM"
                     /locus_tag="ACHKUB_000335"
     CDS             complement(46671..46964)
                     /gene="eutM"
                     /locus_tag="ACHKUB_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO6653412.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(47003..48019)
                     /gene="pta"
                     /locus_tag="ACHKUB_000336"
     CDS             complement(47003..48019)
                     /gene="pta"
                     /locus_tag="ACHKUB_000336"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6653413.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(48016..48819)
                     /gene="eutT"
                     /locus_tag="ACHKUB_000337"
     CDS             complement(48016..48819)
                     /gene="eutT"
                     /locus_tag="ACHKUB_000337"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO6653414.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(48816..49517)
                     /gene="eutQ"
                     /locus_tag="ACHKUB_000338"
     CDS             complement(48816..49517)
                     /gene="eutQ"
                     /locus_tag="ACHKUB_000338"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO6653415.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(49492..49971)
                     /gene="eutP"
                     /locus_tag="ACHKUB_000339"
     CDS             complement(49492..49971)
                     /gene="eutP"
                     /locus_tag="ACHKUB_000339"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO6653416.1"
                     /translation="MKRIAFVGSIGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(49984..50319)
                     /gene="eutS"
                     /locus_tag="ACHKUB_000340"
     CDS             complement(49984..50319)
                     /gene="eutS"
                     /locus_tag="ACHKUB_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO6653417.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(50612..52891)
                     /gene="maeB"
                     /locus_tag="ACHKUB_000341"
     CDS             complement(50612..52891)
                     /gene="maeB"
                     /locus_tag="ACHKUB_000341"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO6653418.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            53180..54130
                     /gene="tal"
                     /locus_tag="ACHKUB_000342"
     CDS             53180..54130
                     /gene="tal"
                     /locus_tag="ACHKUB_000342"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6653419.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            54150..56153
                     /gene="tkt"
                     /locus_tag="ACHKUB_000343"
     CDS             54150..56153
                     /gene="tkt"
                     /locus_tag="ACHKUB_000343"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6653420.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(56249..57292)
                     /gene="ypfG"
                     /locus_tag="ACHKUB_000344"
     CDS             complement(56249..57292)
                     /gene="ypfG"
                     /locus_tag="ACHKUB_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO6653421.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(57418..57993)
                     /gene="nudK"
                     /locus_tag="ACHKUB_000345"
     CDS             complement(57418..57993)
                     /gene="nudK"
                     /locus_tag="ACHKUB_000345"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO6653422.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(58061..60040)
                     /gene="aegA"
                     /locus_tag="ACHKUB_000346"
     CDS             complement(58061..60040)
                     /gene="aegA"
                     /locus_tag="ACHKUB_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO6653423.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            60246..61946
                     /gene="narQ"
                     /locus_tag="ACHKUB_000347"
     CDS             60246..61946
                     /gene="narQ"
                     /locus_tag="ACHKUB_000347"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO6653424.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLCFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            62110..65223
                     /gene="acrD"
                     /locus_tag="ACHKUB_000348"
     CDS             62110..65223
                     /gene="acrD"
                     /locus_tag="ACHKUB_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO6653425.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDDLAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(65322..65381)
                     /gene="ypfM"
                     /locus_tag="ACHKUB_000349"
     CDS             complement(65322..65381)
                     /gene="ypfM"
                     /locus_tag="ACHKUB_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO6653426.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        15442 a        17976 c        16687 g        15436 t
ORIGIN      
        1 tccggcagca accagactta acccaaagac cgttgccgca gcatggctta acagcggttg
       61 cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
      121 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatcgtca tggcgatagc
      181 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
      241 gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
      301 caggaagacc gcttccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc
      361 caggttaggc tgggaaaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
      421 cccgccaatc actttctcca gcggtttttg cccgcgacgt tgcagcatta aaatcaggaa
      481 agtcgcgatc cccgtcagca ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa
      541 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
      601 aacccaatag aaccacacta cgggacgcgg atagtgatca cgaatctgct ccgccagatt
      661 tttaccggtg gcaatcccta gtttggcaga gaggatctga atcagcatcg ccatcaggtt
      721 ggcccaaacg acaacccaca gtagctgata gccgaagcta gcacccgcct gaatattggt
      781 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
      841 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
      901 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ccaaacgaga atgattatca
      961 aattcatttg aatgggttgt ggtgatttct ttgatagacc aggattatga ctacgaaaag
     1021 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
     1081 tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagct tgtcacagga
     1141 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
     1201 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
     1261 cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
     1321 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
     1381 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggat
     1441 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
     1501 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
     1561 acattcgcgt gttgccggtt attatccgcg caattggttt cctgctctcc aaagtcaacg
     1621 gtatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtctgaaa
     1681 actttattgc ctataaagat atcctcggca aaatctcccg taatcgtatg tacaccatgg
     1741 ctgccacggc aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg
     1801 aaccgaaata cgtcgttgct gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt
     1861 cgctgatcaa tccttaccgt gttgatgcca gtgaagaaaa catccagatg tccaacctgc
     1921 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg
     1981 ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac
     2041 tgtttgccac cgttactggc tggtttggct acagcatctc cttccagggc atcctgggct
     2101 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
     2161 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga
     2221 aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
     2281 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
     2341 aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg
     2401 tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat
     2461 cattccccgg cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata
     2521 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac
     2581 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
     2641 catcgcatcc agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat
     2701 atcagcctgc aaacgcttta accgttcgta gttggcatat ccggtgccaa agtcatcaat
     2761 cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt
     2821 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg
     2881 cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
     2941 attgactgaa aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa
     3001 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
     3061 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata
     3121 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
     3181 cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
     3241 ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct
     3301 taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc
     3361 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
     3421 aatcatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact
     3481 acccggcaat tgatacaact tttcgttttc ctgcatcaac ggcagcagcg tacggcaaat
     3541 tgagcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt
     3601 atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
     3661 acgaaagttg ggtaggagcg ttaacggatc tgtcagcgcc tgcaaatgcc agcggcgatt
     3721 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
     3781 cagcaccgca agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
     3841 gttgtaattc agcaggcaaa gcgttgaaac agcccaggtg agatttaaaa acggatagcg
     3901 aagcttaccg accccgaggg taaagatgat gaagaaaacg ggtaccaagt aaccggcaat
     3961 aaagtcgttt tcataaggtg tgcacaacag aagtagcagc acgcttaacg ctgccagcca
     4021 gcttaaggta aatgcgcgtt tctctttgcc caacgacgga gcgatatccc tgcgccacaa
     4081 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
     4141 aatcagcaca gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc
     4201 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
     4261 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
     4321 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
     4381 caacacgaca aacgtctggc taccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
     4441 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
     4501 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
     4561 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
     4621 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
     4681 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
     4741 gcaaacaggg ggccagcctg ttgccgattt ttcatccaat gatgctttca ccaggcggca
     4801 ctaacccgga cgtagactgt attacaaaag cggcaaaaag cagagacaaa aaacccccgc
     4861 tttgcagcga gggttggaaa tttggtggag ctaagcggga tcgaaccgct gacctcttgc
     4921 atgccatgca agcgctctcc cagctgagct atagccccac gatgcgttta cgtaccaagt
     4981 ttgctgggtg caaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc
     5041 atgcaagcgc tctcccagct gagctatagc cccgtcacgt aaagcttgtc gagttgacgg
     5101 gcggcatcat atgaattccg cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa
     5161 tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg ctcgcattca gtagttattc
     5221 atgtcacggt ttcctgtaaa gtggtgttat aaaatgaact actaatagac ccacatacat
     5281 tcagggaatt gttatgttca aggagaggat gacaccagat gaacttgcca gactgaccgg
     5341 ctatagccgc cagaccatta ataaatgggt acgcaaggaa ggctggacaa cgtcaccaaa
     5401 acctggcgtc cagggtggca aagcccgact ggttcacgtc aatgaacagg ttcgtgaata
     5461 tattcgcaat gctgaacgtc cagaaggtca gggagaagcg cctgcccttt ctggtgatgc
     5521 gccgcttgaa gtattgctgg tgacactggc gaaagagatg acgccagttg aacaaaaaca
     5581 gtttacatcc ttgcttctgc gggaagggat tatcggattg ttacaacgct tagggattcg
     5641 cgatagcaaa taatatgaaa agattacgca acaaaatgac caccgaagaa ctggctgaat
     5701 gcctcggtgt ggctaaacaa actgttaacc gttggatcag agaaaaaggc tggaaaacgg
     5761 aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat tctggtcgat acgcaagttt
     5821 gcgagtttat tcagaacacg ccagccttcc ataacacacc aatgttgatg gaagctgaag
     5881 agagaattgc tgaatacgct cctggagcac gcgccccggc gtatcgacaa atcatcaacg
     5941 cgatagataa tatgacagac attgagcagg aaaaagtcgc gcaatttttg tcacgtgaag
     6001 gaattcgcaa tttcctcgcc cgtctcgata ttgacgaatc agcataaata aaaaacggca
     6061 ggatattatc tcctgccgtt tatcttttta cacgctaatt actgctgatt ttcgcgttca
     6121 gcaataaaat ccagcgcttt gttgatacgc tcgatactgc gggtcttacc aattgcgtga
     6181 acggtaacat ccagtgctgg agactgcccc gcaccggtta cggcgacacg cagcggcata
     6241 ccaactttac ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg
     6301 ttttcagcgg tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga
     6361 cgcgctaccg gacgcagatg ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg
     6421 tagaaataac ggcagctctg tgccatctct ttcagcgtct tgcagcgttc gcccagcagt
     6481 ttcaccagat cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc
     6541 cactgtaagt gagtagcaac atactccggc ggcagcgcgt taatgtagtg atggttcagc
     6601 cacagcagct tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt caaagtgaag
     6661 tatttgatca tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc
     6721 accagatagt tcagcagtgc ttctggcaaa taaccgtcat cacgatactg cattacgctg
     6781 actgccccgt gacgtttgga cagtttttta ccgtcatcgc cattgatcat agaaacgtgc
     6841 gcgtaaaccg gcaccggcgc tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg
     6901 ttgatatggt cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc
     6961 acacagaagt tataggttgg ggaaccatcg gtacggcgga taatcagatc atccagctcc
     7021 tggttgctga actcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc
     7081 ggattagcaa aacgcacgac acacggttca tcatcagcat gatgctcatg gctgtggcgg
     7141 cagcgaccgt cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc
     7201 aggcgctctt tagagcaata gcatttataa gcagtgccct cttccagcat ctgatcgatc
     7261 accgcattgt agcgatcaaa acgtttggtc tggtagtacg gaccttcatc ccactccagg
     7321 ctcagccagt tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga
     7381 tcggtgtctt caatacgcag cacgaactca ccgccgtggt tacgtgcaaa aagccaggag
     7441 taaagagcag tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga
     7501 gttttgattt tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg
     7561 gaaaaattga gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt
     7621 tctaccttac gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc
     7681 atcttgctta aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg
     7741 aactctccct ataatgcgac tccacacagc gggggtgatt agctcagctg ggagagcacc
     7801 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaac tactttatgt
     7861 agtctccgcc gtgtagcaag aaattgagaa gtgggtgatt agctcagctg ggagagcacc
     7921 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccact ttctcgccag
     7981 ctaaatttct tgtaaaaatg tgaagtaccg aagtgggtga ttagctcagc tgggagagca
     8041 cctcccttac aaggaggggg tcggcggttc gatcccgtca tcacccacca cttcgggtcg
     8101 ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca
     8161 cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatttgtaag
     8221 attgattttt cttctcaagt tccttgttct tctaccgata ttacctcgta cagtgacgag
     8281 gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac acagagtagt
     8341 aacagggcat cacaaggtag cgatgttgct tcacaaatcg cgcggataag tcagcaaatc
     8401 atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc tgaagacaag
     8461 caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca actggcgcag
     8521 ttgcagaaac agcaggcaga aaaggcgcag gagaaagagc agtgtttgtc tctgaatgta
     8581 tcccttctaa atcctgttga aaaaacgact catatcgata tctatatctg atacaacgga
     8641 tccccttccc gcccagccat tagcgcattg ccctggtctc gtaccacgtc ccataacgct
     8701 tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaaca
     8761 accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag cgccagtgcg
     8821 ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca
     8881 atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt
     8941 cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt
     9001 acataatctt cgacggccaa agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa
     9061 agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg
     9121 acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg caggacaaac
     9181 tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa
     9241 atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta
     9301 tcgcgcaacg tgctgttcag ttcatccagc agtcgctcaa gacgcaaagc cagctgctgc
     9361 cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga
     9421 ttttccagtt ccttcacact gtgactcact gccgactggc tcaggccgat acgctctcct
     9481 gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa
     9541 taattcatct gttatcttca tgaatggatg caataaatca attttatttc tcaaactgga
     9601 agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga
     9661 tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg
     9721 cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc tgttctttat
     9781 gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca
     9841 tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg
     9901 gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct
     9961 cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc
    10021 ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac cattgctggt
    10081 tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg gtaaaattat
    10141 gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg
    10201 ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt
    10261 ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc
    10321 attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt
    10381 ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg tcttttgtgg
    10441 ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt
    10501 gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc
    10561 ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcacagc aggaaagcag
    10621 cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt
    10681 tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga attcatcttt
    10741 gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc ctttatattt
    10801 gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac
    10861 ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgctttcg
    10921 ccagtttaga acctgccgct tctccggcaa tcaccagatc ggttttcttc gacacgctgc
    10981 ccgcgacttt cgcgcccagt tcaaccagtc gagctttagc gtcatcacgc gacatctggc
    11041 ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt
    11101 tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc
    11161 ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag
    11221 gcaccttttg cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg
    11281 ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc
    11341 gagcaaaggt ggtttctttc gctttttcta gcgcgttaac cacgttttgt gccgattttg
    11401 gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tctgccggag
    11461 tatggacata ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat
    11521 ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgct ccgcaaatca
    11581 ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata
    11641 ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga
    11701 cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc
    11761 gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc
    11821 cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct
    11881 gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta aacgctaccg
    11941 cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt gagttgacct
    12001 taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat
    12061 agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaacc
    12121 cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca
    12181 gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa
    12241 gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc ccgcccgtgc
    12301 gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca
    12361 cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatagcg cgaatagtac
    12421 gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg gcactgacta
    12481 aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc tcacagcacc
    12541 aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc
    12601 tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt atctggctga
    12661 aagccgccag cggcgcagcg cctacacgtt gggtaggcga atcaggcgta atcagttctg
    12721 gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa
    12781 tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc gttcgcagtt
    12841 ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa
    12901 gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact
    12961 tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg
    13021 tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg
    13081 aagttctgca gctcgtcacc gtaagacggt acctgcataa agatggtgac acccggagta
    13141 gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctgaat
    13201 aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa
    13261 atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga
    13321 tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga
    13381 gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc
    13441 tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac ctgtggcgaa
    13501 actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg caccggctgc
    13561 gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg gaccggttga
    13621 cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg acgagcagcc
    13681 tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac ctcaccaacg
    13741 ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga cttcattcgt
    13801 tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca gaaaccatgt
    13861 accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg caaatcctgc
    13921 atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt actcactaag
    13981 agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca caaagataga
    14041 tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg cgcaattacc
    14101 cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct gattatggtt
    14161 tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg ctggaagctc
    14221 gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa tattttgttg
    14281 atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc gactctcatg
    14341 agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct ggctgtcatc
    14401 tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat tgccgctccg
    14461 tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac accgccagat
    14521 accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga atggcaaaaa
    14581 tttgcctggt atctgccgcg cgcaattgta ttgctaattc tttacttaat ccctggtatt
    14641 gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt agccatccag
    14701 tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg caccgccctg
    14761 cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt tacgatgatc
    14821 ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc gatgtgggtc
    14881 gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta ttttgtaaac
    14941 cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca tgtcattatt
    15001 tcccttctgt atatagatat gctaaatcct tacttccgca tattctctta gcgggtatgc
    15061 tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga tttttgaaga
    15121 taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg gtaacggacg
    15181 cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc gtatcggtgc
    15241 caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg aactggttga
    15301 accaaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc gcggttacaa
    15361 actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc tgaaagcgtt
    15421 aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa tccaaaaagc
    15481 agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat tcagcaatcc
    15541 ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag ataccgacgg
    15601 tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg gcgtcagccg
    15661 ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg agccaaccga
    15721 ttctccagtt atcgcccagg cactggctgg tgaagagatt aaacctggcc cgcataaaat
    15781 tcagggtatt ggcgctggtt ttatcccagc taacctcgat ctcaagctgg tcgataaagt
    15841 cattggcatc accaatgaag aagcgatttc taccgcacgt cgtctgatgg aagaagaagg
    15901 tattcttgca ggtatctctt ctggagcagc tgttgctgcg gcgttgaaac tacaagaaga
    15961 tgaaagcttt accaacaaga atattgtggt tattctaccg tcatcgggtg agcgttattt
    16021 aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ctgcaacagt aatgccagct
    16081 tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg cttcaaactt
    16141 tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga ctaattattt
    16201 tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt atgatttggt
    16261 tcaattcttc ctttagcggc ataatgttta atgacgtacg aaacgtcagc ggtcaacacc
    16321 cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa ctggcgctaa
    16381 caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac taaacctata
    16441 agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa cggtctgcac
    16501 acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga aattactgtg
    16561 acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac tctgggcctg
    16621 actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa agcggttgaa
    16681 catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta aaaatcagtc
    16741 acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc gctttcggta
    16801 aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt tctgccgacc
    16861 aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca gcccagctgg
    16921 aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc atctttgaag
    16981 ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc ctgattaaag
    17041 ataagcacat gacagctgac gcagctgctc atgaagttat cgaaggtcag gcttctgccc
    17101 tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt gatatcggta
    17161 agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc attcaggatg
    17221 aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg aacctgaaga
    17281 aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct atcatggcgc
    17341 gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag gtgaaaaatg
    17401 acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca accaacgaag
    17461 ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca gagcttgcta
    17521 aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagtg tgcgctaaca
    17581 ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc gttggtctgt
    17641 atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa gaacagtttg
    17701 ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt cgtaccatgg
    17761 acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag aacccgttcc
    17821 tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc gatcagctcc
    17881 gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg atgatcatct
    17941 ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag gaactgcgcg
    18001 acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa acaccggctg
    18061 ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc ggcaccaatg
    18121 atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca cacctttacc
    18181 aaccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct tctcatgctg
    18241 aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct acacttctgt
    18301 tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg cgcattaaga
    18361 agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag gctcttgctc
    18421 aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa aaaacaatct
    18481 gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg tttgttcgat
    18541 aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga gatcattgct
    18601 ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt tgcggaaaaa
    18661 atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc gcctgtagac
    18721 ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc tgatagcggc
    18781 gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga aggcttcaag
    18841 cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga atttgatctg
    18901 ccgctactgg aagagaaagc caagtctacc ctgactccgg ttgttatctc caacatggac
    18961 gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac cccggttatc
    19021 cgcatcaaga agtaattctt gccgcagtga aaaatggcgc ccatcggcgc cattttttat
    19081 gcttccgcca gcggcggcaa aatcaattca tcgctctcat gctgctgggt gtagcgcatc
    19141 acttccagta cgcgcaaccc cgcacggtgc accgcatcgt ttaacgcctt ccctttcagc
    19201 aaaccactga taagctgagc acaaaacagg tcgccagtcc ctttcaggtc ggtttttacc
    19261 cgtgaatggg aaatgacatt cacgctgtcg gcactgacca ccaccacctg catctcctga
    19321 ttttcttcat taccggaggc gctggtaatc accacccatt ttaatgtgtc tgaaagcaga
    19381 ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat ttttaccggt caggatttcc
    19441 agctcaaaga tattgggggt aattccctgc gccagcggca gtaaatattg tcgatacgcc
    19501 tcgggaaggt caggtttgac ataaattccg ctatcaatat cgccaatcac cggatcgacc
    19561 atgatcaata ggtcaggatg gtctttgcgt agcgcagtca gccactcggc aaggattttg
    19621 atttgcgatg ccgttcccat atagcccgtg gttacagcgc gcagctggcg cagcgcatca
    19681 cgctcctgaa gcgcacgcaa ataaccgcta aaccattcat ccggaatcgc accaccgtag
    19741 aaagtgtcat aatgcggcgt attgctcagc aataccgtcg gcacggcaaa aacattcaga
    19801 ccgttctgtt tgatagcagg cacggcaatg ctgttgccca cgctgccgta aaccacctgc
    19861 gactgcacgg cgacgatatc cgcctgcagc gccctgctct tatcgttaaa caacaacaaa
    19921 ctactcattt aattttttct ccttgccgat gatcctcatc gtattccaac cgaaacttta
    19981 cctgattctg gcagtcaaat cggctatcac aaaacaagga taaggtaatt caatgaagaa
    20041 aatcatttgt ctggtcatta cactactaat gacactcccc gcttacgcga agttaactgc
    20101 ccatgaagaa gcccgcatca acgccatgct ggagggatta gcacagaaaa aggatttgat
    20161 atttgtgcgc aacggtgatg aacatacctg cgatgaagcg gtttctcatc tgcgtctgaa
    20221 gctgggcaat acccgtaacc gcattgacac tgccgagcag tttattgata aggttgcttc
    20281 gtcgtcatcg attaccggga agccgtatat tgtgaagatc cccggtaaga gcgatgagaa
    20341 cgcacagcct tttttacatg cgctaattgc gcagacggat aaaacggtgc ctgcgcaata
    20401 atcttaaatc cccgccccct ggctaaaatg ctcttcccca aacaccccgg tggaaaggta
    20461 gcgatcgcca cgatcgcaga tgatcgctac caccaccgca ccagggttag cttttgccac
    20521 ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca cagaatattc cttcccgcac
    20581 tgccagttcg cgcatggtgt tttctgcatc gcgctgatga atatccagca cctcatccac
    20641 cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc cagcggcgaa tgccgggaat
    20701 actgctgcct tcttccggct gcaggccaac aatcgttact ggtttggatt gttcgcgcat
    20761 aaagcgtgaa acgccggtga tagtgccggt tgttcccatg ctggagacaa aatgggtaat
    20821 gcgcccgccg gtttgctgcc agatttccgg cccggtagtg gtgtaatggg cgtaagggtt
    20881 atcgggatta ttaaactgat cgagcagctt tccttcgcca cgattcgcca tctccagcgc
    20941 cagatcgcgc gcaccttcca tgccctgctc tttggtgaca agaatcagtt ccgcaccata
    21001 agcacgcatc gccgcacggc gttcctggct catgttgtcg ggcatcagca atttcatgcg
    21061 atagcctttc agcgcggcaa tcattgccag cgcaatgccg gtgttaccac tggtggcttc
    21121 gattaagaca tcacccggtt taatttcccc gcgcttttcc gcctcgacga tcatcgaaag
    21181 tgccgcacga tctttcaccg aacctgccgg gttattgcct tccagtttta accacacttc
    21241 actgccgtta tccggcccca ttcgctgcaa cttcaccaga ggcgtattgc ctattgtttg
    21301 ttctaatgta ctcacgatct ctatccatac gtggtgttgc ctgatgcgac gcttgcgcgt
    21361 cttatcaggt ctacaggtta caaaccttgc cattaaaaag cccggatcgc gggaagcgcc
    21421 tccgggcgtt taacattcac tcaacctatc aggcgctttg tgcgagagca agttcctcat
    21481 cgcgggtttc gatacgctcg tcgccgttat acagccgcgc atgttgcaga ccaacgaata
    21541 aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac gaccgtcagc ggttcgttgt
    21601 accaccccag cggctgcacc actaattggg tgtagtgacc tttcgggctg gcttccagta
    21661 cctgtaccgg cagcggcgaa tcgagactgg tacggcggct gatatccact tcccacgggc
    21721 gcaggaagag atccaccggc ccctgataag caggtgtgta gcccagcggc cagcgatgtg
    21781 cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag gcggttcact tcgcccataa
    21841 attcgagtac aaaacgggtc gctggttcgc gccatacctg attcggcgca tcagcctgtt
    21901 caatattccc ctgactcatc accactacac gatcggctac ttcggtcgcc tcttcctggt
    21961 cgtgggtcac aaacacgctg gtgaatttca gctcttcatg cagttgacgc agccagcgac
    22021 gcagctcttt acgcacctgc gcatccagcg cgccaaacgg ttcatcaagc agcagaattt
    22081 gcggttctac agccagcgcg cgcgccagcg ccacacgctg tttctggccg ccggaaagct
    22141 gcgccggata acgatccgcc agatgcgcaa gctggaccat ttccagcaat tttgtcactt
    22201 tcgctttgat ggctgcggca ttcgggcgct cgcgacgcgg cagcaccgtc aggccaaaag
    22261 cgatattgtc gaataccgtc atatggcgga acagcgcgta atgctggaac acgaaaccga
    22321 ccttgcgatc gcgcgcgtgc aggcggctga cgtcggtgcc gtggaagcga atatgcccgc
    22381 tggtttgatg ctccagcccg gcgataatgc gcagcagcgt ggttttcccg gaaccggacg
    22441 gccccagcaa cgcgaccatc tgacctgaag gaatatccag tgagatatcg ttcagcacct
    22501 gggtgcgacc aaacgacttc ttaatattgg caatctcaat gctcatgatg ttcctcctgc
    22561 tgtgcgcgtt tttcctgatt ctccaggcgc cactgcaaca tactttttaa aaacagggtg
    22621 ataatcgcca tcagcgttaa cagtgccgca gcggtaaagg agccgacggt gttgtagtcc
    22681 tgctccagca attcaatctg taacggcagc gaaagtgttt cgccgcgaat cgagccggaa
    22741 accaccgaca ccgcgccaaa ctcgccaatc gcgcgggcgt tggtcagcac tacgccataa
    22801 agcagcgccc agcggatgtt cggcaatgtg acacgacgga acatctgcca gccagacgcg
    22861 ccaagcaaaa tcgccgcctc gtcttcctga ctgccctggc ttaacatcac cggcaccagt
    22921 tcgcgcacca caaacggaca cgtcacgaag atggtgacca gcaccattcc cggccaggag
    22981 aacataattt gcagattatg ttcatccagc caaccgccca gcgggccgtt agagccgtag
    23041 aacagcagat acaccagacc ggcaaccacc ggcgatacgg caaacggaat gtccagtagc
    23101 gtcagcagta actggcgtcc agggaagtta aagcgcgtca ccagccaggc cagcagaatg
    23161 ccgaacacca ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag ccagatggcg
    23221 tgtagcatgt ccggatcggc cagattctgt aaaaccggca tcagcccttt gctgaacgcc
    23281 tgtacgaaga tataaatcat cggcaccagc aggatgaacg ccgataccag catcccgatg
    23341 ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt cataacgctt caattgggta
    23401 acttccgcca ttaatgacct accacacgcc gaccaaagcg actttgcaga gtgttaattg
    23461 agaacagcag cagcagagat gccgcgagga tcaccgaagc aatcgcgctc gctgccgggt
    23521 aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt cacttccgtc ttccacgcga
    23581 tatttccggc gataaaaatc accgcgccaa attcaccaag actacgggta aacgacagcg
    23641 ccacgcccgc caccagcgcc ggagaaagct ccggcagcac cactttgcag aaactctgcc
    23701 agcgcgttgc accaagcgtt tccgccgctt cttcatattc cgggcctaac tcttccagca
    23761 ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa ggccatcgcc accgcaatcc
    23821 ccagccaggt ataggtgact ttgatatcaa acttcgccag ccattcaccg taaaaaccgt
    23881 ttacagaaaa gagcgaggct agcgttaaac cggcgactgc cgttggcagc gcaaagggta
    23941 aatccatcag cgcatcaagc agcgtgcggc ctgggaagcg atagcgggtt aggatccacg
    24001 ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac aaacgccgac agcagcgtta
    24061 ctttgtaggc cgcgaccacc tgcgggttgg tgatcacctc ccagtactgc gcccagctca
    24121 tctgggccag ttgcatcacc agcgcggaga gcggcagcag caaaatcagg cacacaaaca
    24181 gcagactggt gccgaggctt aaggtaaagc ccggcagcac gcgtctggag gagacagcaa
    24241 acatcagtta cgccccgccg ctaacagctt gtctaactcg ccgccgctgg tgaagtgggt
    24301 tttcatcact tccggccagg agccaaattt gtcttccacg cggaacagct cggtctgcgg
    24361 gaatttgtct ttcagtttgt ccatcacctc cgggttattc acgcggtagt aatagtcggt
    24421 gatgatggtt tgcgcctgcg ggctatagag ccagttcaga taggctttgg cggctttttc
    24481 cgtaccgttg gcctgcacgt ttttatcaac ccacgccacc gggaattccg ccagaatgtt
    24541 ggttttcgga atcaccactt caaagccctg cgcttcatac tgtttacgga tgttgttcac
    24601 ttccgattcg aagctaatca gcacatcgcc caggccgcgc tcggcaaaag tggtggtcgc
    24661 gccacgaccg ccagtatcga acacttcaac gtttttcagg aactgggtca taaactgttc
    24721 ggttttgcct ttgtcaccac cgtcagcttt atccgctgcg ccccatgccg ccagataggt
    24781 ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt cggagcgcac
    24841 caggtcgttc caatcgtgga tattcttcgg gttaccctta cgcaccagga agcccatggt
    24901 ggagtagaac ggcgagctat tattcggcag gcgcgactgc cagtcggccg ggatcagctt
    24961 gcctttatcg tgcaggattt gtacgtcggt cacctggtta taagtgacaa cgtcggcttt
    25021 taagccctgt aaaatcgcca gcgcctgttt tgatgacccg gcatgagatt gttttatcgt
    25081 cagtttgtcg ccgccgttat cttttgccca ttgttgctca aacggcggat tcagggcggc
    25141 aaacagctcg cgggagacgt cataagaact gttcagcagt tccgttgcct gtacatggcc
    25201 cgccagcagc agagaagcga ccagcgcgag tgagttcttt ttcagtaagt taacggccat
    25261 tgcgcaccct tataaattta atgactttct aatagccatc atatttataa cggacgtgaa
    25321 aggagtaacg gttttatata ccgtttggtg atttggaagt tgaaaaggga ataagacgaa
    25381 atcctcccca cacatgcagg gaggaaacag aggtgaatca gataccgacg ctgaccgtct
    25441 ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag ctggattcat
    25501 ccgatgcgag gaaggccgcc agttcgccga cttccaacgg atcggcgagg cgacgcatcg
    25561 ggattgcttt cgccatttca gtcagcaccg attctggatc ttccgggttc gactggcggg
    25621 caatgctttc cgccattggt gtgcgaacgt atccagggca tatggcgtta acgcgaatac
    25681 cagactgcgc atactccacc gccagcgatt ttgtcaggcc aacaatcgcc gctttcgtta
    25741 aggcgtacgc cgtttcaccc ggatcggcca ccatatcgcc agtgaccgaa gacatcatca
    25801 caatgcgacc atctttgcgg gcaatcatct ccggcagcac cgccttcgtg acgttccata
    25861 cgcctttaat attgatgtca atatggaaat cgcgatcgtc atcgctcata tcgaggaaac
    25921 tgcccagacg acaaacgcct gcgttattca ccaggatatc aatgcgccct tctttttcct
    25981 tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg cacatcggcg acaaccgccg
    26041 tacagcgatg accacgacca cacagttcgt ccgccagctt ttcgatctca ggggagatat
    26101 ccagcaagat taggtttgcg ccatgacgcg caaaagttct ggcaattcct tcgccaattc
    26161 cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt ttaatgctct
    26221 ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata aacaaaatat
    26281 tgctgcggat cagctcacta attcaggaaa atctcaatcc agtcgctgct ttatgcatca
    26341 tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt ttcaatgact ccacatcatt
    26401 gagttgcaca agaccaacaa acagtaaatc ggtaacagaa ttttgcgccg tgcgggtaga
    26461 catcgacgag ctacgccatt cggtttcacc ggaaacggta tcgagcgtga aatgcgccag
    26521 ccgccgcagc ggagaatcag ccagagaagt aatggcaatc accgttgccc cctgctttcg
    26581 tgccgcttct gcgcataaga caatctcttt tttactgccg ctgtaagaga tagcgatttg
    26641 tacatcgcct tttttcaacg cctgggaaac ggtggcttgt acgtgcgtgt cggcttcgca
    26701 ggccacgcga tagccaattt tcatcagttt gaacgacaga tcgcgcccca ccagcgccga
    26761 tccgcccagg cccgttatct ggataaacgg tgctttactg atgacctcaa tgattttttg
    26821 cagccgcgcg tagtcgaaca acgcacaggt ttgctccagc gccagctctt tttcgcgatt
    26881 caatttgcgg gcaatcactt ccagcgaatc atcactggta atcgaactgt gcaggtgcag
    26941 cgccgtggca ttcgttttct cccggctggc actgtattcg cctattaacg ccatacgcaa
    27001 ttcagtaaat ccttgcgcgc cgagtttttg ggcaaatttc actatgctcg actggctaat
    27061 gccgagctgt ttcgccatct gccgtgaaga aacggattgc agctcactca cgttggcctg
    27121 cagaaagtca gcgatttttt gctcgttttc ggtgaattca ctccccgcat tgcttatttt
    27181 cgtcaggtac aacatcttcc actctcctgg gctggcagca taaattccgc ttaagttctt
    27241 atgacgctct tcacactctg cgagtgtaga ctcaatgatt cctttaagtc tgtcaattcg
    27301 ggatatatga ttccatacat ctgcaattat ttgatcgtaa atagtaaggt caccaccgat
    27361 gcaactagaa aagatgatta ctgaaggctc gaacaccgcc tcggctgaaa ttgaccgcgt
    27421 atcgacgctg gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac cgcttgccgt
    27481 tgagcgcgtc ctgccggata tcgccgcggc gatcgatgtt atccacgccc aggtcagcgg
    27541 cggcggtcgt ctgatttatc tcggtgcggg aacatccggt cgtctgggga ttctggatgc
    27601 cagcgaatgt ccgcccacct acggcgtgaa accgggtctg gtggttggtt tgattgctgg
    27661 aggcgaatat gccattcagc acgcggtgga aggcgctgaa gatagccggg aaggtggtgt
    27721 taacgatctg aaaaatatta atttaacggc acaggatgtg gtggttggca ttgctgccag
    27781 cggtcgcacg ccgtatgtga ttgccggact ggaatacgca cgccagctcg gctgccgcac
    27841 agtgggaatt tcctgtaatc cggggagtgc cgtttccacc acagccgagt ttgccattac
    27901 accgattgta ggtgccgaag tggtcactgg ttcttcgcga atgaaagcag gcacagcgca
    27961 gaaactggtg ctcaatatgc tttccaccgg gctgatgatt aaatctggca aagtgttcgg
    28021 caacctgatg gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat
    28081 cgttaaaaac gccaccggat gtaatgccga acaagcggaa gcggcgttaa ttgcctgcga
    28141 gcgcaactgt aaaacggcca ttgtgatggt gctgaaaaat ctcgatgccg cagaagctaa
    28201 aaaacgcctg gatcaacacg gcggctttat tcgtcaggtt ttagacaagg aataacccat
    28261 ggccaaagag atcagcagtg aacttctgaa caccatcctt acccgtgtcg gcggaccggg
    28321 aaatatcgcc agttgtggta actgtatgac gcgcctgcgt ctgggtgtac atgacagttc
    28381 actggttgat cccaatatca aaacgctgga aggcgtgaag ggcgtcattt tgaccagcga
    28441 ccaggtgcag gtcgtttttg ggcctggtaa agcgcatcgt gccgcaaaag cgatgagcga
    28501 gctgctgggc gaggcaccgg tacaggatgc cgcagaaatc gccgcccaga acaaacgtca
    28561 gttaaaagcc aaacaaacct ccggcgtgca acaatttctc gccaaattcg ccaccatctt
    28621 cacgccgctg atccccggtt ttattgccgc cggtctgctg ctggggatag cgacgttaat
    28681 tgccacggtg atgcacgttc cggcagatgc tcagggcacg ctacccgacg cgctgaattt
    28741 tatgaaggtg ttcagcaaag gtttgttcac tttcctggtg attctggtgg gctataacgc
    28801 cgcccaggca tttggcggca cgggcgtcaa tggcgcaatt attgccgcac tctttttgct
    28861 cggttataac cccgctgcaa ccaccggtta ctacgccggt tttcacgatt tctttggtct
    28921 gcccatcgat ccgcgcggca atattatcgg cgtgctgatt gccgcgtggg cctgcgcccg
    28981 cattgaaggc atggtgcgcc gctttatgcc ggacgatctc gacatgctgc tgacctcgtt
    29041 aatcaccctg ctgatcaccg ctacgcttgc gtacctgatc attatgccgc tgggtggctg
    29101 gttattcgaa ggtatgtcat ggctgtttat gcacctgaac agtaatccgt tcggttgtgc
    29161 ggttttagcc gggctgttcc tgatcgccgt ggtatttggc gtgcatcagg gctttattcc
    29221 tgtttacctc gcgttaatgg acagccaggg attcaacagc ttatttccca tcctttcaat
    29281 ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac tggcgggcgc aaccgcacag
    29341 tgcattacgc agtcaggtac gcggggcgat tattcccggt ctgctgggcg ttggtgaacc
    29401 gttgatttac ggcgtcaccc tgccccgcat gaaaccgttt attaccgcct gtttaggcgg
    29461 cgcagcgggc ggtttgttta tcggcctgat agcctggtgg ggtctgccga tgggcttaaa
    29521 cagcgccttt gggccgtctg gtctggtggc gctgccgctg atgacttccg cacaaggcat
    29581 ccttccggca atggcggttt atgctggcgg gattctggtg gcatgggttt gcgggtttat
    29641 tttcaccacg ctctttggct gccgtaacgt caatctggac tgatatcatg aaacggacaa
    29701 tgctctatct ttctctgctg gctgttagtt gtagcgtcag cgccgcaaaa taccctgttc
    29761 tgacagaaag ttcgccagag aaagcagggt tcaacgtcga acggcttaac cagatggatc
    29821 gctggattag ccagcaagtg gacgccggtt atcccggcgt aaacctgctg atcattaaag
    29881 ataatcagat tgtgtatcgc aaagcctggg gagcggcgaa aaagtacgat ggcagcgtgt
    29941 tgatggaaca gcctgtcaaa gccaccaccg ggacgctgta tgacctggcc tcaaacacca
    30001 aaatgtacgc cacgaacttc gccctgcaaa agctgatgag cgaaggcaaa ctgcatcctg
    30061 acgatcggat tgcgaaatat attccggggt ttgccgatag tccaaatgac accatcaaag
    30121 gcaaaaacac gctgcggatt tctgacctgc tgcatcacag cggcggtttc ccggcggacc
    30181 cacaatatcc gaataaagcg gtcgcgggcg cgttatattc ccaggataaa ggccagacgc
    30241 tggagatgat caagcgcacg ccgctggagt atcagcccgg cagcaagcat atttacagtg
    30301 atgtcgatta tatgctgctt ggatttatcg tcgagtcggt caccggtcag ccgcttgacc
    30361 gctatgttga agagtcgatt tatcgcccgc tcggcctgac acatacggta tttaacccgc
    30421 tgctgaaagg ctttaaaccg cagcaaattg ccgccacgga attgaacggc aatacccgcg
    30481 atggcgtgat ccatttcccg aatatccgca cctccactct ctggggtcag gtgcacgatg
    30541 aaaaagcctt ttattcgatg ggcggcgttt ccgggcacgc aggtttgttt tccaataccg
    30601 gcgatattgc ggtgttaatg caaacgatgc tgaacggcgg gggctatggc gatgtgcagc
    30661 tgttcagtgc ggaaacggtg aagatgttca ccaccagctc taaggaagat gccacttttg
    30721 gcctcggctg gcgcgtgaat ggtaatgcca ccatgacgcc gacgtttggc acgctggcaa
    30781 gcccgcaaac ctacggtcac actggctgga ccggaacggt gaccgttatc gatccggtga
    30841 atcatatggc gattgtgatg ttaagcaaca agccacattc gccggttgcc gatccgcaaa
    30901 agaatcccaa tatgttcgaa agcggtcagc tgccgattgc aacgtatggt tgggtagttg
    30961 atcaggtgta tgcggcgtta aagcaaaagt aataaaaagg ccagtcagta atgttactgc
    31021 ctggccttta atcaatgaat cagaaacgct tacagcgcca tcaacttgtc cagcgacggt
    31081 gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc acgcttacca
    31141 tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg cgcgcagtag
    31201 gcgcagaagt acagaccgtg agtgccactg gcagtgccgt acggcaggct ctggcgaaca
    31261 atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga ggtttccgga
    31321 cgttcgtcgc cgtcgatctc ttcgttggcc tctttggtgc gcccgatcat catctcctga
    31381 tcgtgaacgc tcatccggtt gagctgcttc aggttgtgtt cccaacgctg gacaaacaca
    31441 tagctgccgc ccgcacccac gccgtctttg ataaccgcca cttcgcgacg cgtctcttca
    31501 cccgccgggt tttccgtacc gtcaacaaag ccgctcaggt cacgctcttc aacccaacgg
    31561 aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat cgccgcctgg
    31621 gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac atcgaactgg
    31681 gtagtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc tgccccaacg
    31741 ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc aagatgcgcg
    31801 tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact ggccgcacgc
    31861 agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg gcaatgttct
    31921 ggcaaaatgc cactctgaac ctgagacatt gttcctcctg aaaataataa tgccacgcaa
    31981 tccgtggcat tgttgagcgc tattttacgt gttttttcgc ggcggcggtt tgttcagacg
    32041 caaattaacg ccgccagata attttactga ctttccagtt tttcagggta tcgtccgaag
    32101 gcattaaccc ttcaggacca ctccattccc cgctgaactg gtagctaata tgttgactgc
    32161 cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg caattgccaa
    32221 atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg tcagcaggaa
    32281 tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta atcaccatcg
    32341 ccacgttgtc gcccttcatc acttcaaaaa agcgcaccac gttgccgttc gcggttttca
    32401 ttccgctgcg caggcgataa tcgccatcaa gcgcatcggc aatggcttgt tcttgcagtg
    32461 gtgtggacgc cgttaattca ccaacgccct gctcgctcac tttggtggac gacccaaacc
    32521 agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag ccggtcagca
    32581 tcaatggcat agcgcataac attaaacgca gcgatttcat gtcacttcct ttggttattc
    32641 aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt acttcattaa
    32701 aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata tccgccacta
    32761 ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac ggttgccagc
    32821 aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag tacacgccag gtgcgaggaa
    32881 atgtagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaatgcct ggaatcagcc
    32941 ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta ccctgctcgc
    33001 gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat agcacaagcc
    33061 agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata atggacagga tggaactcag
    33121 tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg ttcatatccc
    33181 aggcgttcat acattccgag caccatgtcg ttatcttccg gcacattgat ctgaattttc
    33241 gggcagccac gagcaatcag ctttttctcc agccgattaa gcaacgcatt ggcaatccca
    33301 cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg cccgtcataa
    33361 ccgcccatca ccgttccgac cacttcaccg tttacctcag cgaccaaaaa caaactgacg
    33421 tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg cagcaagtcg
    33481 caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac gcgtatctcc
    33541 atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac ggtcgaatta
    33601 gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt caactattga
    33661 aatgaaaagt aaaacaattc tcaacatcaa accgtcgtaa cggattacgc gatacgatat
    33721 aacatctgga actttattat tacaactcag gccgtatgag cacttttaaa ccactaaaaa
    33781 cactcacttc gcgccgccag gtgctgaaag ccggtttggc tgccctgacg ttgtcaggaa
    33841 tgtcgcaagc catcgccaaa gacgaacctt taaaaaccag caacggacac agcaagccga
    33901 aagccaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac ggcggaattg
    33961 ataccggagc gatcggacgc aacggttcga aagaaaaaca tgtggtgctg gcgattgcta
    34021 aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg cgttctggcg
    34081 atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc gcagatctgt
    34141 ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct tcggtatttg
    34201 ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa cgcgaaaacc
    34261 gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcacctattg cagcaagtgc
    34321 tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgctcggc tcgcatattc
    34381 tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa gccgcgtttg
    34441 tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt atcaccaacc
    34501 cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc acagcgattg
    34561 ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat tcgaaaaagc
    34621 gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac cttcaggata
    34681 cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat agttggcagc
    34741 gcgaagctgg cggcggcggg cgtagtcggg tgttgcgtaa tggtggtgtt ttcgaacagg
    34801 caggcgtcaa cttttctcac gtccacggcg aggcgatgcc agcttccgcc accgctcatc
    34861 gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttcactggta gtgcatccgc
    34921 ataacccgta tgttcccacc agccacgcga atgtgcgttt ttttattgcc gaaaaaccgg
    34981 gtgcagagcc cgtctggtgg tttggcggcg gcttcgattt aacccctttc tatggttttg
    35041 aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgcctgcca tttggtgaag
    35101 acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa catcgcaacg
    35161 aacagcgcgg tattggcggg ctgttctttg atgatctgaa cacgccagat ttcgaccact
    35221 gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta ccaattgtag
    35281 agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc taccgtcgcg
    35341 gtcgttatgt cgagttcaat ctggtctggg atcgcggtac tctgtttggc ctgcaaactg
    35401 gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg gagtatgatt
    35461 atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag gtcagggatt
    35521 gggtgtaact ccctcacccc cattcccgca tccgctgatg caacgtcaac gacggcttct
    35581 cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat ccccactgca
    35641 tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg cggcgtacgg
    35701 cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat gcctaaaata gcgtgaaacg
    35761 cgttttgtag cgtgcggcga ctgacatgca gttgattaca caagtccagc accgtcaccg
    35821 gctcagacat attttccagc acatattcac gggcgcggga aagtaatcta cggtaactct
    35881 gatgactgat gctttccgcc gtcaccattg gttgcgcgtc ttccagcatc gcccccatcg
    35941 ccattagcaa attatccccc agcacttttc gcactgcagg cttatggaga ttttccgggt
    36001 tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct ttatgctgct
    36061 ctttcacttc cagcgccgac tggctacgca gcatatgtaa tacccgatcc gggttatgca
    36121 aaaagttagc ctgccgggtg atgacatctt cagaaagcac cacgcccagg atcgtgtaat
    36181 catccggcgt gctcagttca aattcagtgc caccagggcg ggtggcgatt tccgcgcttc
    36241 ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg ccaaaccaga
    36301 acgagttcgg ccagaccagg cacgactgac gcagcgccag accggtgtat tcacgaaaaa
    36361 cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc ggatgcagct
    36421 gatcgtaaat ctgctgccag gcggtaatcg ttaaagcatg ttcatagaca tccgttgtct
    36481 gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt tcgggtaagg
    36541 gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg ttaatgccgg
    36601 gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga taacgactac
    36661 tgcgtttacg taacgtcccg gcagaaaaga gctgttcgag cgcatttctg gctttttcca
    36721 gcggccagcc aaagtgggca gccacttctc ctgccgtcat tccctgacgt actgatgtta
    36781 acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg ggttccctta
    36841 cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca tcgtctggcc
    36901 gaccgatctc cttgcggctg atgacacgcc cggtacgcat cgcggcagca caacctgcgt
    36961 ccagcgccgc acgacacgcc gccagatcgc cttccaccac cagcgttaag cgaccagggt
    37021 caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca tccgctgcat
    37081 ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc atcggcaacc
    37141 tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg gtaaaggcgt
    37201 tacaggctgc tttacacgcg gcctggctac cggttaaaaa tgctgccgag tagttggttt
    37261 cagacggtgg cgggacatag gtcaccagct gtacgtcggc agatttcaac gctgcatcaa
    37321 tgccataggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg ccaagcgtga
    37381 tcccggcggt tgatgagaga taagaaccgg tacgcgaaac cacatgcgcc aggaaggcgg
    37441 tgttttctgc gtcattggcc cactggaatg ctgcaccatt ttcaatatgt gcaaccatcg
    37501 catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc agcacttcac
    37561 cggcagtcgg tgacgggccg tgtgcagcgc ccgcatacag cgagcgacca tataccactt
    37621 ccaccatcgc ctgcttggtc gcttcatcag cggcaatgta ggtgacgtca tcggaatcag
    37681 cagaaatcag cccgaggcta cgaatatgcg gcggcaattt tagttcacgc gcaaattcag
    37741 cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa tctaaagctg
    37801 gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc agcatacgtt
    37861 tcgccaaatc cacaatgacg gctgcggctt caaccggcgg cgtgccgccc tggtgaatgt
    37921 tagagataca ggtgcgatcg gcctcaacgg tggtcgccat acgcggcgag taaacggcgt
    37981 agcaggagag gctttctgac tgccctaacc ccggacgttc ccccaccagc aggatcacca
    38041 ctttcgcgcc gaggatctca ccgatctgat cttcaatctt cacgcgacca taacgaacaa
    38101 agaatggcgt gccgactttc agcccggcct gtttcaggcc cgccatcagc ggcggcagga
    38161 tctcttcgta gttcacggtg atggcatcag ttgacaggcc atcagaaata acgacctgta
    38221 cgtccggatt agcaacacac tgtgctttca gcgcttcaac agcttctgca cacaggcggc
    38281 ggcccatatc cgggcgagtc aggtagagat ttttgtcgct aatttcagag cgtacttcca
    38341 gcaagccctg cgctttcacc cactcttccg gcacttcttt cagtacggta tctttcgaac
    38401 gagagtgatc ggccaggaaa cgcagcagcg cctgggtacg tggacgcgga ccggcacgac
    38461 cggtacaaac gcgggccacg gtgctgcggc gcagttctgt taatacgtct gcgcgatgcg
    38521 ggttttcaac accaatccac gcttttgctt cagcggaacc taaatccagc gcacagcttt
    38581 ccgaggtcac cggtgccgca cagttggtgg tggcgcactt tgcttctgac ggcgcggggg
    38641 ccgtttgtcc cattgacgcc atcacgctgc gtacaatttc ttcaatctgt ttttgatcca
    38701 tgatgtgtta tctccgcgtc atcagaagaa cagtgacgga tcgcccgccc gtttggtcag
    38761 gcgaccgttt gccataatgc ccatgctttc cagccagcgt tcaaactccg gtgacggacg
    38821 caggttgagt aactgacgca cagtggcggt atcgtggaat gcggtggtct ggtagttgag
    38881 catgatgtca tcacccagcg gcatccccat gatgtagttg cagcctgcgg tggcgagcag
    38941 gatcatcagg ttttcgttga ggttctggtc agcgtcagcg tggttggtgt aacagcagtc
    39001 acagcccata gagatgccgc tcagcttgcc cataaagtga tcttctaagc cagcacggat
    39061 aatctggcgg tcgttgtaga gatactccgg cccaataaag ccgaccacgg tgttgacgat
    39121 aaacggatcg taatgacgcg ccagcccata gttacgtgct tccatcgtca cctggtcagc
    39181 gccgaagtta gcgccagcgg atagcgcaga gccttgtccg gtttcgaagt agaggcagtt
    39241 ttccccggcg atacggttga actctgcacc cactgcgcgc gcttcgtcga gcatcgccag
    39301 ttccacgcca aactctttca gccctttttc gctgccacag atactctgga aaatcagccc
    39361 gcctggtgcg ccgcgacgaa tcgcttcgat ctgggtggtg acgtgcgcca gtacgcagcc
    39421 ctgagttggg atgttgaatt tgtcgatcac gccataaatg gtatccagca cgcggcttaa
    39481 gttttccacg tcgtctgtca ccgggttaac gccgatcacc gcatcgccca ctccgaagga
    39541 aagcccttcg tagatttgcg cggcgatact ttgcacgtcg tcacgggtgt catttggctg
    39601 caaacgggcg ctaaaggtgc ccggaatacc gatggtggta ttggcctttt tgattaccgg
    39661 cattttcttc gcgccgtaga tcaggtccgc gttggagcaa atcttcgcta ccgccgcgac
    39721 cacttccgag gtcagccctt tgcgggtaaa ggcaatgtcg tccacgctgg tttcatcgct
    39781 cagcacatac tcacgcagtt cgctgatgct ccagttttta atctggttgt aggccgtttc
    39841 gttaacatcg tcctgaatca gccgcgtcac gcagtcatct tcataggcaa tcaccggatt
    39901 attgcggatg tccgctacgg tcatttccga caacacctgc tttgccgcca cgcgctcctg
    39961 tgaacttgcc gctgcaacgc ctgccagcac atcccccgaa cgcagttcgt tggctttagc
    40021 cagcacctct tttacatcct taaactgata tacattgccg aacaatgtgg tctttagttt
    40081 cataagtcgt tccctcagga aggaaatgcg agtgatttca ccgtcaccgg cacaaccgat
    40141 ccgccaaaaa gaggcgtacc aatgtcgata tagtcccccg cgcggacaat cacttcgtca
    40201 atgactgcca acgggagttg ttgtagctgt gggcgcaaca acatgcccag agctttacca
    40261 aagtcctgcc cggccaccac cagcagggga tgcggattcg gaaaacgcgc gacgaaatcg
    40321 accagcgcgt tgatgaccgt cagtaccgcg gcgtaacgca caggcagcga ggcgggaagc
    40381 gccagcacgt acgcgtcagt tttgggatca agatccagct gaatcagcgc ctgttgccag
    40441 gcactcacca gatccgtttc atcaattggg atcgccaccg gcaaattgcg cagcggcagt
    40501 tgtacgccct ccagccagat tgtgctgcca gagagcgaaa gggtatgtgc gcccgcgcca
    40561 atcaccgtgg cgcgtacggt ttgcgccgga aactgcacat tcatctcacg caggcgcgga
    40621 tggtcatgca gcgccgttgc cagcagcggg ccaatatcgg caaaacagaa cggatcggcg
    40681 ggctggtggc gataacattc gcccacgccg ccagaaagcg taatgatttc gggcgtaaca
    40741 cctgcgggca gcaaaccggt ttgcattaat gcctgcgcga gcggcgaaag cgttccgtca
    40801 atcacttcga caatcagctc tgccatccgc cgggtaacct gcaccagctg cgcgccggtc
    40861 agcgaacggg cgtcggtgcc tgcaccgaag cactcatcca caatcatctg ccccggttta
    40921 tgagcgtaaa ccacgcgccc ctggctgtcg gtttccagca ggcgaccgcc gacgttgagg
    40981 caggcagtgc cgctgatttt tccggcatcg aacagggcgt agttcgcggt gccaccgccg
    41041 atgtcgatat tcagtacccg acacagccgt tgttcagaaa gggtttgcgc cccggcaccg
    41101 tgaccggcga tcacggattc gaggtgcggc ccggcgctgg caacgacaaa atcgcccagc
    41161 gactgagaga gcgccatcac cgccgggcga gcattgcggg ttttcgcgct ttcaccggtg
    41221 atgatgatgg caccagaatc aacgctttcc ggcgcaatac ccgcagcctg atattgctcg
    41281 agtattaagg ttttcagttc cgcttctttt aaaccgccct gtttatcgac aggggtaaag
    41341 aacaccggac tttgccagct aatttcgcgt ttaatgaatt cgtagcgcgg cacctgcgac
    41401 accgccgcac ggttaaccag ctccaggtgg gagaaaatca cctgggtggt ggtggtgccg
    41461 atatcgatac cgacgctcaa tagctggcga gtgttcacga ttgtgcctcc gcttcggttt
    41521 tagtcgcggt cgcgtcttct tttggcacca gcatcatcgc cacgccaatc gccgttacgc
    41581 cgccgatcaa cttgccgaca atcatcggga agatcatggc gttcatgttg gcagcggcga
    41641 agcctaagtg gtcgcccagg gcgaaagcag cggaaacggc gaaggcgcag ttgatgactt
    41701 tgccgcgggt atccatctgc ttcatcatgc cgaacatcgg gatgttgttg gcaagcgttg
    41761 ccaccatgcc ggctgccgcg atgttgttca tattcagtac tttaccgacg ctcatcagcg
    41821 gtttttcaaa ccagcgagtc agcagcagca ccattggata cgcccctaac agcacgcagg
    41881 agatagaacc gataacttca atggcgcgca tcacttcacc gggtttatcg ccaggggcca
    41941 taaagatagg atccagaccg gggatcagtt cccagccaag caggaatttc actaccgcag
    42001 cggcaagacc gagggtgatc aatgcaacga ggaatttggc gaagatctgg aagccgttga
    42061 tcattttttc cgggatgaat ttcagcccca gcgccaccag aatcgcaaca atgatcaccg
    42121 ggatcatgtt catcaggatc agggcgaaag tgaattccac cggctgaccg ttgatctgca
    42181 caccggagta catagcaacc agaccaccag caatacagcc aatcggaatg gtcacaatgc
    42241 ccgccagcac gccgagcgcc agataacgac gatcagaagg ttcgataatg ccgagcgcca
    42301 ccggaatgga aaacacaatc gttggcccca tcatcgaccc gagaattaac ccagagtata
    42361 gccacgctgc tacgtcgccg cccgccagct ctttggcgag gaagaagccg cccatatcgc
    42421 acgccagcag cgttccggcg aacattgacg ggtttgcgcc gagcatttcg taaaccggga
    42481 taattaccgg cccgagtacg tgagccagta ccggtgccag cgcggtcata ccgaccatcg
    42541 ccagacccag agcgcccatt gccataaagc cttcttcgaa ctgaccgcca gatccttcga
    42601 tacttttacc gaacttaccg aggaaacgag cagaaccgcc gaactgcgac aggatcctgt
    42661 ctacggcagc tatcagcata aagaacatca tgatgtacat gatgatttcg ttaattccca
    42721 tcgcctttac tccctgttag ttgttattta ttggcggatg cggcgtaaac gccttatccg
    42781 ccctacatgt gcaatcccga aggccggatc agacgcggca agcgtcgcat ccggcattta
    42841 ttgcgccgct gcgtacaagc cgacaatctg ctccaggctg gcggtacgcg ggttgctgcg
    42901 cagacaaata tcttcctgcg cggcctgcgc ccatgcaccg taatgcgcag atgtcgcgcc
    42961 aacatcgccc agtcgtttac caatcccaac ttccgcaatc agctcactta ccgcgttaat
    43021 agcgtcatga tcgtcggatt ttttagttcg cagtgcccga ccaatctgac taaagcgttc
    43081 acgacaaacc atccggttaa attccatcac tgttggcagc aacatggcgt tcgccagacc
    43141 gtgcggaata tgcagcgccg cgcccggctg atgcgccatc gcgtggcaca acccaagacc
    43201 cgcactggaa aacgccattc ccgccataca tgaagccagc aacatgctct cgcgcgcggc
    43261 aaggtcgtga ccgtagccca ccgctttcgg cagcgatttg ccaatcatcg caatggcacc
    43321 aatcgccaga ctgtcggtaa acggtgtagc gttcagggcg ctgtatgctt caatggcatg
    43381 ggttaacgca tcaatgccgg tcatcgccgt gacatgcgac ggcacacctt cggtcaatgc
    43441 ggcgtcgagg atcgccacat ccggcatcag cgaggcatgg gctaacacct gcttgcgccc
    43501 gctcaccgcg tcgataatca ccgttacatt ggtggtttca gagccggttc cggcggtagt
    43561 tggaatggca atcagcggca agcgcggttg cagaacgctg gtttctgaca tctctgccag
    43621 cgtgctatcc gggttcgtca ccagcaacgc cacggctttc gccgcatcca gcaccgagcc
    43681 gccgccaaat gcgatcaccc cgtcacaacc tgactcacgc aactgcgcca cggctgcaca
    43741 cacgtcggta atgcacggtt cgcccaccgg acatggccag agcgtcatgg cgatgccttt
    43801 aacagccagg ctgcgcgtca gcccggcggt catccctgcc tgatgcaaaa agctgtctgc
    43861 catcacgaac agatgtttca gcccacgcgt ttgcgcttgc tgtccgcaac tgctcaccga
    43921 gcccggaccg caaagcgtca ccggtggaac gctaaatgtt tttacccgtt gcagattcag
    43981 ggtatcgaac gcctgaaaga gcgcggtctg caattcattt tgcatatagc ccctccgctt
    44041 tctctcgccc actactggcg atcgccagcg gggtcataaa caaactgtgc tgcggtaaat
    44101 gcacttgtaa cgccgggaat tgtttgcgaa acagttccgc cacgcccggt tgcatacagg
    44161 agccgcccgc cagccataaa tcagtaatgc cctgcccttc aatatgccgg gcgacaatgt
    44221 ccgccatttt ttcgtacacc ggtttcaccg cgggccaaat ctcgtcacca tgaccgcgct
    44281 tgtactgctc cgcctcttcc agcgaaatac ggcgatttcc ggcgagagtc agagagatgt
    44341 gatgtccgcc ggtggcttca tccgccgagt acgtcacttt gccctttttc acgatggcaa
    44401 tgccggtagt gccgccgccg atatccacta caccggcgtt gtccagttgc agcagatccg
    44461 caactgcggt tggctcatcc agcacatggc tcacttccag tccagcagac tccagcacgt
    44521 tgatggaaat acgcgggtcc gtacctggag gaaatgaagt cgccgcatgg ctaaaacggc
    44581 gaccgaattg ctgctcgagc gtgtcgagat ggcgacgaac aatggtgaca gcgccgaaga
    44641 aatcccagac gatgccgtcg cggaccacat cggcccagtc gaggcatacc gccaccggct
    44701 gaccgtcgcg gtcaacaacc atcgacacca catcgcaggt gcccagatcc acccccagcc
    44761 acagcggaga ttccgtcgcg gcaggcgtct ggttacacag cgttgccgcc gtttgcagac
    44821 gtggggtgag ccattgttcg tcgtgcgcca tcggttactc cttaaacaat gcgaaacgca
    44881 tcgactaata cacagcgacg cagacggaca aacgtccgcg cgctggttac cccttcaccg
    44941 gttggcgtgg tgatggtcat ggtggtccag ccttccccgc ccagccccag cccggcaatg
    45001 cacggtccgt tcttaacgaa aatgctggta tcaatggcgt tcgccatctg gttcatgttt
    45061 tcgatattgc gcgagtgcat tgccgccgtg tggtggcaac cgccctccag tttcaccgcc
    45121 agcgcaatgg catccgccac gttggcgacg cgcacgacgg gcagcaccgg catcatcagt
    45181 tcggtcacgg caaacggatg ttctgcagtg gtttccacaa acagcaaacg cgtttcttgc
    45241 ggcacgttca ggccgattgc cgccgcgatt ttcgccgcat cgcgaccaac ccagtcacgg
    45301 ctgacggtgc cttttccgcg ctcgtcgata tttttcagca acaccggttg cagctgctgc
    45361 gcctgttctg cggtcagttt caccgcgtgc tggccttcca tcagacgcat cagttcatcg
    45421 gctacgctat caacaacaat cagtaccttt tcgtcggcac aaatgatgtt gttatcgaaa
    45481 gaagcgcctt tgacgatgga ctgagcggca cgggcgaggt cggcggtttc atccaccact
    45541 accggcgggt tgccagcgcc tgcggcaatc agacgtttat tggtgtgttt acgcgccgct
    45601 tctactaccg cttcgccgcc ggttaccacc agcaggccga tacccggaaa cttgaacaag
    45661 cgttgcgcgg tttcgatatc cggatttgcc acagtaacca gtaagttttc cggcccacct
    45721 gcggcaacaa tcgcctggtt gagcagcgta atcgcccgct gggagacttt tttcgccgcc
    45781 ggatgcgggg caaaaatgac gctgttgccc gcggcaatca ggctgatggc gttgttaatt
    45841 acggttgccg ccgggttagt ggaaggcgtc accgaagcca ccacgcccca gggtgcgttt
    45901 tcaattaggg tcaggccgtt gtcgccagtc agcacttgcg gagagaggca ctcaacgcct
    45961 ggtgtgccgc gcgcctgagc gacgtttttt gcaaatttat cttcaacgcg ccccatgccg
    46021 gtttcactga cggcaagttc cgctaaatct ctggcgtgtt tttcgcctgc ttcacgaatg
    46081 gcagcaatgg ctaactggcg cattgccacg ctttttaacc cttgctgggc gactttggct
    46141 gccgcaacgg cgtcatccag ggacgcgaaa acgcccatct catgaacggc ggcgggcggc
    46201 gtgtcactgc tttgcatttt cagcagtacc gctttcacca cctgttcaat atcctgttga
    46261 ttcatgatgt tctgccttat ttgtggaaaa ttacctgacc gccagacacc acctcatcga
    46321 caatgccaat cacgcacaga tcgaccggtg acgtttcgct tttatgcgcc tggcgggcgg
    46381 aactgccact caccagtaac acccactccc cggttcccgc gccaatattg tcgatggcga
    46441 cggcgcattg cccgtcagga ttaccttgtg gatcaatcat ttccaccatc agcaatttgt
    46501 catgcgccag tccgtgatgg cgtacggtac aaacaatttg tccagtgacg actgccagtt
    46561 tcatacccgc ctccgtggcg tatttcaggt aaaagctccc cctaccctcc gcagaaggta
    46621 aaatgaaaaa ggagagagcg tgacgcccga atcgacgtca cacagggtga ttacaggttg
    46681 ctgctatcgc ctttcaggcc gatcgggaag acttcttcca ggtcgccgtg cggacgcggg
    46741 ataacgtgta cagataccag ctcgccgata cgctgtgccg ctgcggcacc tgcatcggtc
    46801 gcggctttac aggctgcaac atcaccacga accatggcag tacacaggcc gccgccaatc
    46861 tgcttaacac caaccagctt gacgcgcgca gctttcacca tcgcgtcaga agcctcaatc
    46921 agtgcaacca ggccccgggt ttcgatcatt cctaatgctt ccattgtgct ttcctcttta
    46981 tcagggtcca gaacgggacc gttcattcaa ccagtgtttg taaactgctt tcgcggttca
    47041 cttctgtctg acgcggcaca gctgccacca gcgccagctc gataatttcc tgcacgctac
    47101 aaccacgaga gagatcgtgc atcggcgcgg caagtccttg tatcagtggc ccgacggcac
    47161 gatatccgcc gagtcgttgt gcgattttgt aaccaatatt tccggcttcc agcgacggaa
    47221 aaaccatcac attggccttg ccctgtagcg ggctggcagg cgctttttgc gccgccactt
    47281 ccggcacgaa ggcggcgtca aactgtaact cgccatccac caccagcttt ggtgcgcgct
    47341 cacggacgat ttctgtcgcc tgctggacgt tagcaacaca ggggtgacgg gcgctaccat
    47401 tgctggaaaa cgacagcatc gccacgcgcg gctcttctcc ggtgatggcg cgccaggttt
    47461 cggcactggc aagcgcgata tccgccagct gcgccgccgt cggctgtggc accacgctgc
    47521 aatcggcaaa gcccaacgcc gggccgctgt actgtggcaa catcaggaaa atggaagaga
    47581 gcgttttaca gcctggctgc aagccgataa tgcgtaatcc ggcacgcagc acatttgccg
    47641 tggaagagag gttgcccgcg atacagacat ccgctttacc ggcgctgacc attgctgcgg
    47701 cgaacatcag agggtcggta agtttttcca gcgcatccgg cggcgttttt tcgcccgcgc
    47761 gggccagcca gcgatgagca aattcttccc gcattgcgag gttgccatgc ggatcaatca
    47821 cctgtagccc gtccatcgcc acgccgtgac tgagcgcaaa ctgacgaagt tcaaacggat
    47881 tggcgaccag aatgggcgtt gccagacctt gttgatgtaa atattgcgcg gctttcagca
    47941 cacgttggtc taacgcatcc ggaaaaacca ctctggcggg cgctcgcaac gccagttcac
    48001 gacaacgttc aataatcatt gcgtttcccc cagtcgttgt tggatctgct tcaccgttag
    48061 cggctgcttc gttacgctca ggatcatcat cacgtagacc gtgctggaaa ggcggttcag
    48121 cgcctgcaaa atgtccgggc gtaatacttc aaagctgcgg gtgataaaca cctgcgccgc
    48181 tacggtttcg gtttcccgca ctttggtacg tagcagattg agcaacgccg catcgcgccc
    48241 gtggctggct tccggcacca gatgatcgtg gtcgagatag cgcagcggct ggtgagaaag
    48301 tcgatgcaga tcttcgtcag aaagcccgac aatcgcctgg cagcccagcg gttcacccag
    48361 cgcatcggcg cgcataatgt tgccaagacg cgaacggata tccgccagcc acggctgcca
    48421 cggttccgcc agttcaattt gcagccacac tgccagcgca atggtgctgt cgagaaccgc
    48481 gcgaaaaccc agacgcggat cgcttttggc gaccattttt tccgccgaca ggtgagtcag
    48541 cgtatccggc ttttttgcca ccggctggcg gcacagttcg cagcacgcct gcggatgttc
    48601 atcgctactg gtcagcccat gaacgggctg cggctgctgc tgttcatcgt caacaaacag
    48661 gcggccctgc tcgtcaataa acttgatgcg cagatggcgg ctttccagta actcccgggc
    48721 agagggcgtc aggcgactgt ccgcagggag atggatctct gcgccttcgc tgagcgtatg
    48781 gttcgctctt agccatgctt cggtgatgaa atctttcata gggattgcca gttagccggc
    48841 caggcgacat acaggaattt cacgctggat gtcgtaccaa attcgatact ggagcctttc
    48901 gggataaaca tcacgtcacc cgctttggcg atcatggttt cgccttcgtg gcgaacatgc
    48961 agctcccctt ccagcaccat gtcgatttcg tcgtagttca gggtccacgg gaagaatgcg
    49021 ttctcccact gcataaaacc agcggccatg ctgctgccgt cgtctccggt cacaagatca
    49081 gtcaagccca cgcaatgcgg ctctgcgccg tcgaaacgac caaacttgac gctgctgcca
    49141 tcaatcactt tgatgccgcc tttgccggtc accgatttga agctcggctg cattgcgccc
    49201 tgctccagcg actgtttttc cttcatcact ttttccatca gctgtgcgac caggctttcg
    49261 gtaaactggc cttccggcag ctgggcaatg atggtttcgc ggatgcgctg gctttcggtt
    49321 ttgtcggctg gcacgctggc gggcacggac gccgttaccg ggatcgattc atcacattcg
    49381 gtgatggtaa aacccagtag atccgccact tcacgggctt ccggggtaat aatgctggcg
    49441 cgcagaacca cggacattgc ctgttcgccg cgcgcgtgtg cttcacgaat atcattcgct
    49501 gtgatgagtt ttttcacctg cttcctcctg tttggtgaga gatgccagat aatccaccag
    49561 ttgctgcacg ctttgcgggt cgtggctgtt tagctcgaaa atcggctctt cgaaacccgt
    49621 ctcaagcaat aactttcgcg ttgcggcgac gtcagcatct ggcatgtccg ttttactgat
    49681 gacggcgatt tgccgtttgc tgacgccaat atccagcaac ccggcaggca accgactttc
    49741 cgggtcattt gcgccatgga catagatcag catgtctaca tcctgtagcg tggtaattaa
    49801 ggcgtgatac cagcggggat ggctaaaata ttcacccggc gtatcaatat cgcccttatc
    49861 attaaattcc acggcctgtg tttttctggc gagggtataa ttcccctgca acgcattaaa
    49921 taacgttgtt tttcctgccc caatgctacc cacaaacgca attcgtttca tgaatgcctc
    49981 gtattaactt ttggtcattt cacaaagcgt ataatttaat aagcgaccta aaccgctgac
    50041 ggtttgtgat aacgcttctt ctactgcgcc caccgagcca taaatcacca atgcaccgct
    50101 gaagcggtcg agaaaaccga tatgcacatc agcggctttc agcgctaaat caccggcgat
    50161 catcgccgtt tcgccgggag ttagcgtcat aatgccaatt gcgcccgcat ccggaacgcc
    50221 gattttcttc gccagttcct caccgggatg cgcgatgaga tgcgccagtg tgacctgttt
    50281 gccgggcaca aattcctgaa tgatgcgttc tttatccatg agtcgccgcc tgtgaaaaac
    50341 tttcgtgtat cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta
    50401 aaaggtgagg tagatcacta agaatggaga gatattcgct gtggtgcata aactttcata
    50461 tgcaacgcat gaatataaat aaaataatat cgagatatta tatagactgc tttttttatt
    50521 attcgcaaac tcagtaataa gctttactaa tatattgcag ccaaaccgct taccctgaat
    50581 attcagggta agcgtgagag ttaaaaaaga attacagcgg ttgggtttgc gcttcaacca
    50641 cggccagcgc caccatgttg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa
    50701 ccggtttcgc cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc
    50761 gcagtaagtt gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag
    50821 aacctttcaa agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat
    50881 cgccgtgcat ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg
    50941 cctgacgcat tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca
    51001 gagcaacgcg cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct
    51061 ccgccagctc ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac
    51121 cactcggcag cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa
    51181 agacattttt caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg
    51241 catcggcttc cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg
    51301 cccgctgcgc ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc
    51361 agtactcttt aaagcgcgga tcggattcgt tattgacgat ctcaaaatca acgcccgctt
    51421 tgatctgcaa gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga
    51481 tcggtttcgc cagtcccagc gttaccagtt cctgagtggc atgcagaacg cgcgcctctt
    51541 ccccttctgg cagaacaacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca
    51601 taaacaggtt ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag
    51661 caatcggacg cgtcgccacg cccgactcca tcgcggcttt agcgaccgca ggagcgatct
    51721 taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat
    51781 cctgatcgcc atacgctgaa gccaccactt cgctctgttc ggcgtgggcc agttccgcaa
    51841 tcgcgcgtac cgccgccagt ttcatctctt cattgatagc cgttgcgcca acatccagcg
    51901 cgccacggaa gataaacggg aagcaaagga cgttgttgac ctggtttgga tagtcagaac
    51961 gaccggtgca aatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg
    52021 gttccgggtt cgccagcgcc aggatcattg gcgcacgagc cattttcttc accatttcct
    52081 gggtcagcac tttcgggccg gaacatccaa ggaaaatatc cgcgccttca atcacatcat
    52141 cgagggtacg tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatgtttg
    52201 gctcacggcc ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca
    52261 gacccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca
    52321 ccatccgcac gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag
    52381 tgctgatgat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc
    52441 gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgattccgc
    52501 cgaaggtcgg ttcgagcgca gcgacaactt caataaattt gtccgggtcg agttcatcaa
    52561 cttcaatgtc aaatacatca atcccggcga atttcttaaa cagaacgccc ttgccttcca
    52621 tcaccggttt gccagccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag
    52681 agatcaccgc caccaggtta ccgcgcgcgg tatatttgta ggcttttaac gggtcttttt
    52741 cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg
    52801 ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat
    52861 caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca
    52921 aataaagtgt gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg
    52981 agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat
    53041 cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt
    53101 ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac
    53161 ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt
    53221 ggcagacagc ggcgatattg agtccattcg ccattatcat ccccaggatg ccaccaccaa
    53281 tccttcgctg ttactcaaag ctgccggatt atcacaatat gagcatttaa tagacgatgc
    53341 tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga
    53401 caaactggca gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtc gcgtgtcaac
    53461 agaagttgat gcacgcctct cttttgataa agaaaagagt attgaaaagg cgcgccatct
    53521 ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc
    53581 gacctgggaa ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct
    53641 gacgctgctg ttttcttttg cccaggcgcg ggcctgtgca gaagcgggcg tttttctgat
    53701 ttcgccgttt gtcgggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta
    53761 tgtggtggaa gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca
    53821 acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc
    53881 cttaaccggc tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa
    53941 agtttcgcca gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc
    54001 catgagcgaa gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact
    54061 gtctgaaggc attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc
    54121 caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc
    54181 gcgcactcag tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg
    54241 gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc
    54301 caacctggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct
    54361 acagtttgct acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc
    54421 agttgcattc gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca
    54481 ccaccggccc gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta
    54541 cactggcggc gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt
    54601 ttatgggcga cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca
    54661 cgctgggact gggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg
    54721 aaacagaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg
    54781 tgatccatga aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc
    54841 aaagcgtgaa agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc
    54901 cgaataaagc aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc
    54961 tggcacggca aaaactgggc tggcaccatc cgccatttga gatccctaaa aagatttatc
    55021 acgcctggga tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg
    55081 ccgcctataa aaaggctcat ccgcaactgg cagaagagtt tacccgacgg atgagcggtg
    55141 gtttaccgaa ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc
    55201 cggcgaaaat cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc
    55261 tgcctgagtt gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag
    55321 gttctgtttc gctgaaggaa gatccagcgg gcaactacat tcactacggg gtgcgtgaat
    55381 ttggcatgac cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg
    55441 cgacgttcct gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga
    55501 aagcgcggca gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga
    55561 cgcaccaggc tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc
    55621 gaccgtgcga tcaggtggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg
    55681 gaccgacggc actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc
    55741 aggttaaaga gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata
    55801 ttattctgat tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag
    55861 caggagaagg tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc
    55921 aggatgagga atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg
    55981 aagcaggtat tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga
    56041 tgacgggtta cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg
    56101 ccgagaatat tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat
    56161 cgccggatgc tgattgccgg atgcgacgct gacgcgtctt atccggccta cagcgtctta
    56221 tccggcctac atgtccccgc aattttgttt aacgggtgat ccacaacgtg ggccaggcgt
    56281 ctggcccatg ccagttatcg caggtgggtt ctgcggcata acgcaccagg cgaaaacgct
    56341 gaccgtcaaa gcgccagcgc gcctgaatgc cacaatcgct taatccgcgc cctttcgcta
    56401 aggtcaccag ttcacgcgat ttctcatcaa atgttgcgtt catcagttcc agttcattcg
    56461 tctcctgacc gttgttgaac ggcaaacgca accgaaccgg gcgcgaggct agtggctttt
    56521 tacgcgacac aatccatgcc aaatcaatgg tgttataggc ccctgcctca cagctaatca
    56581 tcatcagcgc tttatcatcg gtcagcgcag tgacattcac ctcacgacgt aacggatcaa
    56641 gcgagcagcg cagaccattc atccgccagt ttccataatc cagcaaatcg ttgcgttctt
    56701 cgagtgagag tggcgtcggc gttgggttaa ccaccgcgac ctctttcagc gcaggcgcgg
    56761 gcggtacgct gagcggcggt tcgtcccctt tcttgatcca cgcggtttca ctgccaacgc
    56821 gcttttgctg agcatcaata aacaacaacg ccgcttttaa gccactcaga gaaatggtct
    56881 gattgccgtc gcgtaaggtg atcgccttcc cttcctgaat catctgcaaa aacgcggtga
    56941 tggttgccgt atcatcggtt actaataacc atggtgaaat ccgccacttg tcaccactta
    57001 acgccaacgg ctcaccatct aacagcagcc gtggcgctat ctctccttca gacgcatccg
    57061 gcgacttcaa cccgccgcgc tcaatacgta aaacggcatc ggtatgcgcc ccggcgctgc
    57121 ggctcagggt catcaccagt ccattatgat cgcccgtatt gcgcgccacg cagaagtttt
    57181 ggttattaca ggtgacctgc cagtcggaaa acgcccgttg cgctggtgcc gcccacacca
    57241 aagacgtcgg caacaaagcg aaaaaaaaga gaaggaaaat gcgatagcgc atggacggta
    57301 cgaccccaga agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag gtagcgcggc
    57361 tcaatcagat ttgtcggatt gagccaataa taaacaacga aatattattg ttatttttca
    57421 gtccattaaa tgtgacgttt gcaaatagtt aagcaataac accgtcttac cgtcacgtat
    57481 ctcgccggtt ttgatcatct ccagcgcctg gctgaacggc agctcgagca cttcaatatc
    57541 ttcatcttca acaccgccac cggcgttagc gcgctgattg tcactgtatt cggcgataaa
    57601 aaagtggatt agctcagtca caccgccagg cgacatatac agttcaaata atttgcgcac
    57661 ttcgcccact tcatagcccg tctcttcaat cgcttcttta cgaatgcaca cttccggttc
    57721 gtcgttatcc agcagcccgg cgcaggtttc aatcagctgc ccgctttcat tgccattaac
    57781 ccaggtagcg acacggaact gacgaatcag aaccacggtc tttttcttcg cgttgtacag
    57841 gaggatcgtc gcaccattac cgcgatcgta aacttcacgt ttatggcgga taacttcgcc
    57901 atctttgcgc gtgagatcgt aagtaatgtt gtgcagggtg aaatagttat cggagagaat
    57961 tttgtcttta atgagggtga tttgttgcgt cataccgact ccacagcgcg aaatgaacaa
    58021 ttatattacg ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat ttgactgatt
    58081 ttacccccag ccagtcaata atcccctgtg ccgcatgacg tccttctgcc attgcggtaa
    58141 ccaccagatc cgcaccacgc acggcgtcgc caccggcgaa gattttcgga ttggtggtct
    58201 ggtaacggta ctggctttcc acatccgcga tgatgcggcc ccatttgtct accgttacac
    58261 cgtgcgactc cagccacggc atcccgtgtg gattgaagcc aaacgccata atcaccgcat
    58321 cggctggcat gacaaattca ctgccctcaa tcggtacagg tcgccgacgc ccctgggcat
    58381 ccggctcgcc aagacgcgtg cgcaggaaac gaatcccgca gacgtgacct tgttcattca
    58441 gctcaagcgc caccggctgg acgttaaatt cgaagttggc cccctcttcg cgggcgttcc
    58501 tcacctcttt cttcgagcct ggcatgttag cttcatcacg acgataagcg caggtgacgt
    58561 tactcgcgcc gtggcgcagc gcagtacgca cacagtccat cgcggtgtcg ccgccgccca
    58621 gtaccacgac gttaagtccg gcggtattga taaacggctc ttccggtagc tcttcgagcc
    58681 ccatcacctg tttagtgttg gcaatgagga acggcagcgc gtcataaacg cccggcgcat
    58741 cttcattggg taaacccgct ttcatggaac ggtaagtgcc tacgccaacg aagaccgcgt
    58801 cgtattgttc caaaagcgaa tccaaagaga catctttacc cacttcacaa ttgagttcga
    58861 agtgaatccc catcgcgctg aagatttccc gacggcgtgc cagcagggat ttatccagtt
    58921 tgaaagaagg aatgccgaaa gtgagcaagc caccaatttc tggatggcga tcgtacaccg
    58981 tcaccgccac gccattgcgg gtcagaacat ccgcacaggc cagccctgcc gggcctgccc
    59041 cgataatcgc cacccgcttg tccactttgg tgacatgact taagtcagga cgccaacctt
    59101 tcgccaacgc ctgatctgaa atatagcgtt caatgttgcc gatagttaca gcaccgtgct
    59161 catcgcgaat agtacaggca ccttcacaca aacggtcttg cggacaaacg cgtccggtaa
    59221 tttccggcag ggtgttggtc tggtgagaaa gctcgacggc ggcgtcgatg tttccggctt
    59281 tcaccagttc aatccactgc ggaatatggt tatgcagcgg gcaggtccat tcacaaacgc
    59341 tgtgctcgcc gcacttaagg cagcgcgagg cttcccgttg tgcctggtcg gcgcgaaatg
    59401 gcagataaat ttcatcaaaa ccggttttgc gcgcttcaat cgccagttta tccggctcgc
    59461 cacgcgcggg cgttgcctgc atttgttcga ctttactcat taccggcatt tcttgcgccg
    59521 cggtactggc atgccacggt tgatgttcct gacgcgcggt gcgcaagcgg cgggatttcg
    59581 ccatgccgga gagtgcgacg tcagtgacca gttgcagcgc gtccgccggg caattctcaa
    59641 cacaggcagg accgttttcg cgccccgcac aaaggtcgca tttatgcgcc gtggctttta
    59701 cttttcctgc cgcgacgggt gtcaggacga tttgcatcgt accaaaagga caggccacca
    59761 cgcaggattt acagccaata cacttttgct gattgacctg aatgctgtca tcaacgtggc
    59821 tgattgcgcc attagggcag ctacgggcgc agggcgcatc ttcacaatgg tgacaggtca
    59881 ctgcactacg ttgctgttga tgtttgataa ccgtaattcg gggatgaaaa tggtgttggc
    59941 tcaggacatg ttgctcatca ttgtgagcca tgacacaggc gatttcacaa gcatgacaac
    60001 ccagacattg ctgactgttg gccataataa aacgattcat aacgaccttc ttttttggtt
    60061 gtaaaaacct tattctttat atgagtgttg ttattacccg acttacaggg gaatcggcaa
    60121 tgttcatgtg cccagaataa gtaactattt cgctataaac tgtggcagat caaataatcc
    60181 catcactgac taaattgtgt ttcagctaac tggaacatta atgatttttt gtggagaaga
    60241 cgcgtgtgat tgttaaacgc cccgtctcgg ccagtctggc ccgggccttt ttttacattg
    60301 tgctgctgtc gattctttcc acgggtatcg ctctgctaac tctggcgagc agtttgcgcg
    60361 acgctgaggc tatcaatatt gccggatcgc tacgtatgca gagttaccgc ctgggctacg
    60421 acttgcaaag tggcagtcca caactcaatg cacatcgcca gttgtttcag caggcactgc
    60481 attcaccggt attaaccaat ctcaacgtct ggtatgtgcc agaagcagta aaaactcgct
    60541 atgcgcatct gaatgccaac tggctggaga tgaataatcg gctcagcaag ggcgatttgc
    60601 cgtggtatca ggccaatatt aataattatg ttaatcagat agacctgttc gtgctggctt
    60661 tacagcacta cgctgaacgc aaaatgctgc tggtggtggc gatttccctg gctggcggta
    60721 tcggtatttt cacgctggtc ttttttactt tgcgccgtat acgccatcag gtggttgccc
    60781 cgctgaatca gctggttacc gccagtcaac gtattgaaca cgggcagttc gactcgccgc
    60841 cgctggatac cagtctgccg aatgagcttg gtctgcttgc aaaaaccttt aaccagatgt
    60901 cgagcgagct gcataaattg tacctttcgc tggaggcgtc agtagaagaa aagacccgcg
    60961 atctccacga ggccaagcgt cgtctggagg tgttgtatca atgttcccag gcgctgaaca
    61021 ccagccagat tgatgtgcat tgtttccgcc atattttgca gattgttcgc gacaatgaag
    61081 cggctgaata tctggagtta aatgtcggtg aaaactggcg gattagcgaa gggcaaccaa
    61141 acccggaatt gccgatgcag attttaccgg tgacaatgca agagacggtt tacggcgaac
    61201 tgcactggca aaatagtcac gtttcatcat cagaaccgct gcttaacagc gtttcgtcga
    61261 tgctgggacg cggtttgtgc tttaatcagg cgcagaagca ttttcagcag ttattgttga
    61321 tggaagaacg tgcaaccatc gcccgcgaat tgcacgactc gctggctcag gtactttctt
    61381 acttacgtat ccagttgacg ttactgaagc gttcgatacc ggaagataac gccaccgcac
    61441 aaagtatcat ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag ttacgcgagc
    61501 tgttgactac tttccgcctg acgctgcagc aggcggatct cccctccgcg ttgagggaaa
    61561 tgctggatac gttacaaaat caaaccagcg ccaaactgac cctcgactgc cgtctgccaa
    61621 ccctggcgct ggatgcgcaa atgcaggtgc atttgttgca aattattcgc gaagcggtgc
    61681 tgaatgcgat gaagcacgcc aacgccagcg aaatcgccgt cagttgcgtc accgcgccgg
    61741 acggcaatca cacggtttat atccgtgata acgggattgg catcggtgaa ccgaaagagc
    61801 ccgaaggtca ttatggtctg aatatcatgc gcgaacgcgc ggaacggctg ggtgggacgc
    61861 tgactttttc acaaccttcc ggcggcggca cgttagtgag tattagcttt cgctctgcgg
    61921 agggtgagga aagtcagttg atgtaatgcc tcctactgac caaagaatac ttgcacttaa
    61981 ggttcagtat aaaagggcat gataatttac attaactcct ttttttctcc acgattggct
    62041 cgtaccttgc cgctacagtg aagcaagtca agcctacaac gatacgcaga aacacgaggt
    62101 cctcttttaa tggcgaattt ctttattgat cgccccattt ttgcctgggt gctggcaatc
    62161 ctgttgtgtc tgacagggac cctggcgatt ttttcattgc ccgttgaaca ataccccgat
    62221 ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc ccagacgctg
    62281 gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg gtctcgataa tctcatgtat
    62341 atgtcatctc agagcagtgg caccggtcag gcatctgtca ctttaagttt taaagcaggc
    62401 accgatccgg acgaagccgt gcagcaagta caaaaccagc tgcaatcagc catgcgaaag
    62461 ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta aaaccggcga taccaatatt
    62521 ctgaccattg ctttcgtctc taccgatggt tcgatggata aacaggatat tgccgattat
    62581 gttgccagta atattcagga cccgttaagc cgcgtgaatg gcgtcgggga tatcgatgcc
    62641 tatggttcgc aatattccat gcgtatctgg ctggacccgg cgaaactcaa cagtttccag
    62701 atgacggcta aagatgtcac tgatgccatt gagtcacaga acgcgcagat tgcggttggg
    62761 caacttggtg gtacaccttc cgtcgataag caggcgctca acgccaccat taacgcccag
    62821 tcactgctgc aaacaccaga acagttccgc gatatcacct tgagggtgaa tcaagacggc
    62881 tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa atacgattat
    62941 cttagccgct tcaatggtaa gccagcctcc gggctggggg taaaactggc ctccggcgcg
    63001 aacgaaatgg cgacagcgga gctggtgctc aatcgtctcg acgatctggc gcagtatttc
    63061 ccgcacggac tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa agcctccatt
    63121 gaagacgtgg tgaaaacgct gctggaagct atcgccctgg ttttccttgt tatgtatctg
    63181 ttcctgcaaa acttccgcgc cacgctgata cccactatcg ccgtgccggt ggtgttgatg
    63241 ggaaccttct ccgtacttta cgccttcggt tacagcgtca acaccttaac catgttcgcg
    63301 atggtactgg cgatcggtct gctggtggat gacgccatcg tggtggtgga aaacgtcgaa
    63361 cgtattatga gtgaggaagg actcactcct cgcgaagcca cacgtaaatc gatggggcag
    63421 atccaggggg cactggtcgg gattgcgatg gttctttcgg cggtatttgt accaatggcc
    63481 ttcttcggcg gcaccaccgg tgccatctat cgccagttct ctattaccat tgttgcggcg
    63541 atggtgctgt cagtactggt agcgatgatc ctcactccgg ctctgtgtgc cacactactt
    63601 aagccactga aaaaaggtga gcatcacggg caaaaaggct tttttgcctg gtttaaccag
    63661 atgtttaacc gcaacgccga acgctacgaa aaaggggtgg cgaaaattct ccaccgtagc
    63721 ctgcgctgga ttgtgattta tgtcctgctg cttggcggca tggtgttcct gttcctgcgt
    63781 ttgccgacgt cgttcttacc gctggaagac cgtggcatgt ttactacctc ggtacaattg
    63841 cccagcggtt caacccaaca acagaccctg aaagtcgttg agcaaatcga gaaatattac
    63901 ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca ctgttggttc tggccccggg
    63961 ggtaacgggc aaaacgtggc gcgaatgttt atccgcctga aagactggag cgaacgcgac
    64021 agtaagaccg gcacctcatt tgccattatc gagcgtgcaa cgaaggcgtt taacaagatt
    64081 aaagaagctc gcgttatcgc cagcagcccg ccagcaatta gcggtcttgg tagttctgca
    64141 ggttttgata tggagttgca ggaccacgct ggagcaggtc acgatgcgct gatggcagca
    64201 cgtaaccagt tgctggcgct ggcggcggaa aacccggagc taacccgtgt gcgccataac
    64261 ggcctcgatg acagtccgca gttgcagatt gatatcgacc agcgtaaagc tcaggcgctg
    64321 ggcgttgcta tcgacgatat taacgacaca ctgcaaaccg cctggggttc gagctatgtg
    64381 aatgacttta tggatcgtgg tcgcgtgaag aaagtctatg tgcaggcagc tgcgccgtat
    64441 cgcatgctgc cagatgacat taatctctgg tatgtccgaa ataaagatgg cggcatggtg
    64501 cccttctctg ctttcgcgac ctcacgctgg gagacaggct cgccgcgtct ggaacgctat
    64561 aacggttatt ctgcggttga gattgttggg gaagccgcac cgggggtcag taccggtacg
    64621 gcgatggata ttatggaatc gttagtgaag cagttgccaa acggctttgg tctggagtgg
    64681 acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct gtacgccatt
    64741 tccttgctgg tagtattcct gtgtctagcg gcattgtatg aaagctggtc ggtaccgttc
    64801 tcggtaatgc tggtcgtgcc gctgggggta atcggcgcgc tgctggcaac ctggatgcgc
    64861 gggctggaaa acgacgttta cttccaggtg ggcctgttaa cggtcattgg tttatcggcg
    64921 aaaaacgcca tcctgatcgt cgagtttgct aacgagatga accaaaaagg ccacgacctg
    64981 tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc cgattctgat gacctcgctg
    65041 gcatttatct tcggcgtatt gccaatggca accagcacgg gtgccggttc cggtggtcag
    65101 catgcggtgg gtactggcgt aatgggcggg atgatttcag ctactattct ggctatttac
    65161 ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc gcgcccggaa
    65221 taagcaataa aaaaggcgac atgccaatgt gtcgcctttt tcaactttcc gattaagaac
    65281 ctgctcagcg ggttcttgct gtttatactt tgtctcggga attacttacg aagcataact
    65341 tcgataaagt ctttccagtt ccccagttca cgttcaatca taacaacctc tcttataatt
    65401 atgggtattc tacggaaaca atataccgtg gtgaagctaa tttactcgat tgctgcgatg
    65461 actacctccg ggggacaaac cttatgtaaa tactatggtc ctgcggtgat aatttgtatg
    65521 tgatacacag caacatttcg a
//
LOCUS       DAZFRN010000006        60785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFRN010000006 DAZFRN010000000
VERSION     DAZFRN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.006"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            714..1175
                     /locus_tag="ACHKUB_000350"
     CDS             714..1175
                     /locus_tag="ACHKUB_000350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653427.1"
                     /translation="MIKQAWEYMDAATVIKNAAGIRADDRAALLVTADGRTVQGLAKQ
                     LPEELAGKLEDLAKSMMPHTQPERHYTNDAEVQRLLQSLRMCVVQEFIRTSRGEFDAA
                     AIYSKGKLAAARVLTEVHGIPAAQVAEKCAFAISDWRWEVSRAIREANRNG"
     gene            1168..1335
                     /locus_tag="ACHKUB_000351"
     CDS             1168..1335
                     /locus_tag="ACHKUB_000351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653428.1"
                     /translation="MGNRTVMFCGVSYTVPAAFGTLSRDELEYAITLMRRGDFDTLRE
                     ACAWVAGESDE"
     gene            1332..1496
                     /locus_tag="ACHKUB_000352"
     CDS             1332..1496
                     /locus_tag="ACHKUB_000352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653429.1"
                     /translation="MTVITWDELEELWEMWGTDYAAYWHSLGRDEERPSFFEYVQALG
                     YIPQDAILAD"
     gene            1635..2162
                     /locus_tag="ACHKUB_000353"
     CDS             1635..2162
                     /locus_tag="ACHKUB_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO6653430.1"
                     /translation="MTTEITITAPSVYVGTYAKYNNGSIAGKWINLLDDVTDRDDFYE
                     KIAELHSDEADPEFMFQDYENMPRGIVSETSINWNYIDGLRRVLEENNEEWAEAYALF
                     VENFHDTDVENFYESYCGTADSEADYAEQLLDDTGELNAIPRNLRYYFDYEKFARDLF
                     INDYTFIDGHVFRDY"
     gene            2248..2826
                     /locus_tag="ACHKUB_000354"
     CDS             2248..2826
                     /locus_tag="ACHKUB_000354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653431.1"
                     /translation="MNKYIEMPSHMLRAALVCVDDNSSRLVFTGVHITPEIMEASNGH
                     VAVRMTHGCQCDRDITVIFKGKIPRAAEVTRIHPGDTPIAEHINKKGDVIGMTACQVL
                     DAEWPSARLAQHFPDKTDTSTVATLDTRYLTFPDKMFGTGQGRKQIGCAVCPAPAGCA
                     MRFRFDRATTKLFGNPVFIVMPIRYDGETGLE"
     gene            2920..3303
                     /locus_tag="ACHKUB_000355"
     CDS             2920..3303
                     /locus_tag="ACHKUB_000355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653432.1"
                     /translation="MTDFNEQPPVVTRSGTVHAPNLIHKRDGWHLSYCGSSAAYGCKT
                     TALVIDNRVFFVLKGDHRREWMTARTLWEALQYFVAHDDQIHPASEHRMSLGLDVDSF
                     GLMPTLRTALTSEQFNLLREYFAEL"
     gene            3417..3938
                     /locus_tag="ACHKUB_000356"
     CDS             3417..3938
                     /locus_tag="ACHKUB_000356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653433.1"
                     /translation="MTGRSSAWLVVHTATGYADRAAYTRDEMAALDIMADDSGGELYL
                     ARVKDRVHAEAVLAWCSLLAFGTWSLVRTDIYKRDGLFVELWPDTGKFIGYAGARAAA
                     VALNAAHQDEGFVWELIPEHPDVRDGLAALRRYVVAIQASKDLSRWPCFGDDIRPYTR
                     RRGGRCVTVWRAV"
     gene            4075..4326
                     /locus_tag="ACHKUB_000357"
     CDS             4075..4326
                     /locus_tag="ACHKUB_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFO6653434.1"
                     /translation="MTHEILAYTAASISDLQRNPVETVAAGDGGAVVIFNNNEPAFYC
                     VPPELYAYYRELAENEELNAIADERANETDSIRVSLDDL"
     gene            4316..4597
                     /locus_tag="ACHKUB_000358"
     CDS             4316..4597
                     /locus_tag="ACHKUB_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6653435.1"
                     /translation="MTYEIRFLPRALKEWSKLGHTVREQFKRKLAERQDNPRVQADAL
                     RGKPDRYKIKLRSSGYRLIYEVRDTEIVLLVIAVGKREGDLVYDLADKR"
     gene            4978..5511
                     /locus_tag="ACHKUB_000359"
     CDS             4978..5511
                     /locus_tag="ACHKUB_000359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653436.1"
                     /translation="MKYWTVTTHDGRVGVVYAEQVIPDNVVTMVSAEWAGTEVVQNDG
                     MKASLAVLYVLRDGIVRSAQITPVIAGGAPLDDLYRRSTFVQKYNGAWRDASGDWELS
                     LRDRSAVQPWDNSMARMEAASLMLLDEAEGRGAWPALINAITCPRYAPNISGVMRQLP
                     AGTRVWDTRYQRVVVAG"
     gene            complement(5615..5689)
                     /locus_tag="ACHKUB_000360"
                     /pseudo
     CDS             complement(5615..5689)
                     /locus_tag="ACHKUB_000360"
                     /inference="COORDINATES: protein motif:HMM:NF044641.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5783..6040
                     /locus_tag="ACHKUB_000361"
     CDS             5783..6040
                     /locus_tag="ACHKUB_000361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653437.1"
                     /translation="MQQLYVGVGMFGTVEECVARLSARAPGERVMVTVWTVEDVWEER
                     PDLTAEQAEDVLRVVNRRYGTGDWSTLAEIATDLFGPQVAE"
     gene            6051..6242
                     /locus_tag="ACHKUB_000362"
     CDS             6051..6242
                     /locus_tag="ACHKUB_000362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653438.1"
                     /translation="MAVIQYVPFSKAVKVMEKAGVTVRAAEDFLAACDEAKGVPMDEL
                     DAFIHGEALEHRAFLTDEE"
     gene            6247..6666
                     /locus_tag="ACHKUB_000363"
     CDS             6247..6666
                     /locus_tag="ACHKUB_000363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653439.1"
                     /translation="MRTEKQIRSWRDLQIAYFAHHPVSGSYTAPETPSGWCSRIGTHK
                     ARMEYRGTRDDGCPCRTVFTVESTRWNSKYSACNVSVAIEMSPDCGHEDTCRHRESCH
                     RREMVSIKHDGTRVMRAIRDEIDAWSGSGTITKSKVL"
     gene            6676..6960
                     /locus_tag="ACHKUB_000364"
     CDS             6676..6960
                     /locus_tag="ACHKUB_000364"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO6653440.1"
                     /translation="MVLPETISRDAAQMLALEIHREKELIYRNIGTDYHTLAEYAVSL
                     EREQSYRAAYYAWAAALARARGVNVAWCEARMKYCGFNGGLISRAQCAAQ"
     gene            6975..7304
                     /locus_tag="ACHKUB_000365"
     CDS             6975..7304
                     /locus_tag="ACHKUB_000365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653441.1"
                     /translation="MYKCREVSVPAELLETANAEIAAALGTLYEVCKKYNLPMTATVI
                     DTQFQDADGGWHTGMSSSRYAAGDCITTVIQACVSEGLYTQAMQLLAEIVVQEALDDG
                     AEKPVCH"
     gene            7273..7608
                     /locus_tag="ACHKUB_000366"
     CDS             7273..7608
                     /locus_tag="ACHKUB_000366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653442.1"
                     /translation="MTGLKSQYAIKGDNIMAALKDEDIVEYGLAEYQDIYTAAEALLD
                     KGKDAGVPALLFLAPVTAPGRCTLSAAGVCLDKPRTLRSFDAAARSYAEILKIMESHE
                     DSVASPTKH"
     gene            7844..8122
                     /locus_tag="ACHKUB_000367"
                     /pseudo
     CDS             7844..8122
                     /locus_tag="ACHKUB_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
     gene            8338..8559
                     /locus_tag="ACHKUB_000368"
     CDS             8338..8559
                     /locus_tag="ACHKUB_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653443.1"
                     /translation="MTIVKTHTGTVVTRDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLLDTIKLIEVQDHYGKLGV"
     gene            8562..9002
                     /locus_tag="ACHKUB_000369"
     CDS             8562..9002
                     /locus_tag="ACHKUB_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653444.1"
                     /translation="MMTFTREQLATLREASEAAKEKTTWQSLQAFRAAATPEVVLALL
                     DVLDASREAEPVAWKAVFTQVDQESNTFIAMYSDKAEVERWVRLHEIGDFRAEITPLY
                     AAPVVPEEISTTHTMLPAMNDQLMAFGYVKGWNACRAAMLKGGA"
     gene            9169..9672
                     /locus_tag="ACHKUB_000370"
     CDS             9169..9672
                     /locus_tag="ACHKUB_000370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653445.1"
                     /translation="MMKRWMPLLSVIVAQVFFGVMVMRVPELSMLWQWSVIGAFAMAC
                     VASFIVGRITGQEREQVRSALLCFYAAYQTWLCSGAPNRSPFSRRHGLCVNLWDYCED
                     AGFPMWVIRAACVQLHKDFARAGRNAQLPFNADNMSYSAESYQQVCHENPARIAWVND
                     QLQQLTE"
     gene            9684..9866
                     /locus_tag="ACHKUB_000371"
     CDS             9684..9866
                     /locus_tag="ACHKUB_000371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653446.1"
                     /translation="MTDNQKVFCVLVMIVVTAILVGIFAVEGPVPAWAVVVNHLMSAA
                     TWAYVGCVFIGKKFYG"
     gene            9859..10323
                     /locus_tag="ACHKUB_000372"
     CDS             9859..10323
                     /locus_tag="ACHKUB_000372"
                     /inference="COORDINATES: protein motif:HMM:NF024363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HFO6653447.1"
                     /translation="MAEYKSPVVHKLKILPKYFSAVCAGTKRAELRKNDRDYRAGDVL
                     HLVEAYPGGDSGILTGQQLSVSVTHVADVSAYAPGHVLLSIAPVWIPCSERMPPVGVD
                     VQLFVSSTGEQFTGFNLDNTGDFQYADCFGAPIVCEATHWMPLPEPPIKQGE"
     gene            10328..11137
                     /locus_tag="ACHKUB_000373"
     CDS             10328..11137
                     /locus_tag="ACHKUB_000373"
                     /inference="COORDINATES: protein motif:HMM:NF037120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653448.1"
                     /translation="MGKKYELVKGQSVVVEGVTLYRIRALRDIPWRCVNAGDLGGYVE
                     SERNLSQGGNAWVYGDAKVSGDARVFGDAQVHDKAVISGHARVSGRAQISGEACIRGM
                     AQVCGYALVRDRATVCGNAIVMDEALVEGVAEICDHAVVCDDATVKDANIRDQVSVAG
                     DACVNGHVVIMGIALLYDCACVRSCEDWLTVSDFAGTVCFIDDYGQNIECLHLDGPLT
                     AYRSGEGDIIVRAEGYMDTLHEFDECGDATCKALAAFIRAQMNGETGGTGE"
     gene            11226..11456
                     /locus_tag="ACHKUB_000374"
     CDS             11226..11456
                     /locus_tag="ACHKUB_000374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653449.1"
                     /translation="MVSNKAVRNVMALTGLILMFLGIVLAALGVLLTAYHWTVGNPVM
                     PWLYVTGVALCALLAGSFLMEYGGIKPMPDKR"
     gene            11503..11901
                     /locus_tag="ACHKUB_000375"
     CDS             11503..11901
                     /locus_tag="ACHKUB_000375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653450.1"
                     /translation="MTDSERIAQLERECAALQKVLERFVEFAGKVQPMYKEWEGGTGK
                     FAGVSAAEQPSAPSPGQRKHITYAMAHEVRRERAEHPEESVRALARRLNIPESTVRSC
                     LKLDDRRMRELKKKDMRSADEAYRLTGGDA"
     gene            11901..12797
                     /locus_tag="ACHKUB_000376"
     CDS             11901..12797
                     /locus_tag="ACHKUB_000376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6653451.1"
                     /translation="MSYMKIWRDLWTTSDLAQKRIENLSAVITQINTQFIPKIHDRLA
                     ASERHVKKFDPMLERWENGYLVRTRVVRQRVEVPVAVDSGEHDDVSSQWRRVRRIDYA
                     TYQEVMRLTEEGLGIHRIGKRLGISPQTVSNTRKMPPERVEKLRQLYQGDSGVDGGER
                     KDEVNESKPQRKPQKIVDYEIYQKVMDMLNSGLSAYRISKELGFSQQTISNIRHMSPE
                     RVERLRRLYENGEQVRPCYIPTPFDGDTLKKIISIQKDSPNIPLSRLSALVGVSVYHL
                     KKYLALSDAERDALLEALDEDE"
     gene            12784..13557
                     /locus_tag="ACHKUB_000377"
     CDS             12784..13557
                     /locus_tag="ACHKUB_000377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653452.1"
                     /translation="MKTSELCDAYDRLQQRVENLSAVITQINTQFIPKIHDRLAASER
                     HVKKFDPMLERWENGYLVRTRVVRQRVEVPVGDSEAAKTEHAIRGRRTRKEADYGTYM
                     KVVQMLNDGLTAYRIGKELGISHQMVANYRNMSPERVEHLRLVSEGKVALYDNRVRGM
                     ARPLTNDEVQKVLDIQATMPDIPLSRLSVLTGVSVYRLKKYMALSDAERKALLSAVTD
                     TTPVVNTASDVTATQSRTRKSRKGDDLNDPVFFSDDSDM"
     gene            13567..13770
                     /locus_tag="ACHKUB_000378"
     CDS             13567..13770
                     /locus_tag="ACHKUB_000378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653453.1"
                     /translation="MTNEEALEKARASVLAVNESLDMLEQKTAELVQQCEGIVTRMNA
                     VEQKLKQIEKMHADVQRIMSGKK"
     gene            13817..14734
                     /locus_tag="ACHKUB_000379"
     CDS             13817..14734
                     /locus_tag="ACHKUB_000379"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6653454.1"
                     /translation="MRKIYEAEIKRAKAAEARGEKHTPAVQKVSRYAVDPRTIRIIEG
                     FNIRKLSQEHIDSIAKSYAAGQPVPPVLVAVAADGVLELVDGEHRVRAAIQADMQRID
                     IQVFYGTEEERVAVAVSSSQGRNLTPDERALAYKRLSDKGWSNDEIAEKVGRSAGDVA
                     NHLLLAQCGPKVLEYVASGEIKATPLFSLAREIGPAAVLDRVEQALARKRAAATPPPE
                     APVEEKKKSGRGTKPGSTKAPRPRIRASDLTLFGKKDYERVTEIVAGLRFTSEITSDD
                     ADVMLLCRGELARELKELLNRYAVAKGEE"
     gene            14738..15286
                     /locus_tag="ACHKUB_000380"
     CDS             14738..15286
                     /locus_tag="ACHKUB_000380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO6653455.1"
                     /translation="MTAHVMVDMETAGTGPDAAILSAALVQFIPETGEIVSSFAVDID
                     LNSAVKHGGVIEPGTMQWWAKQSDTAREVFNSTDAVPFPLAMYSIQEWLDATGPAYVW
                     SNGAGFDCVILRRALERLGYKAWPFCMDCDVRTMVMLGQQTGFDPKKDMPFEGTEHNA
                     LDDALHQVKYVSAIWQRLVGGA"
     gene            15283..17553
                     /locus_tag="ACHKUB_000381"
     CDS             15283..17553
                     /locus_tag="ACHKUB_000381"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO6653456.1"
                     /translation="MMTRLVCLDFETFYDKDYSLSKMTTAAYIRSPLFQVICMTATVD
                     DGPVLEAWGYDDVRQLLLSLELDRPDTTTVAHNARFDGAIIEWIFGIRINRLLCTILM
                     MRQVGLSRLIRESLASVSDFLRQRGYPIPAKGHEVERAMGQRLEQMTPEFRAAYGAYC
                     RNDTVILRAVAQLLMPECPDDALRAMDMTLTMYTRPVFVLDKPLLEKYLVTLREKRQA
                     ALQEMARQYGFLTTDDLLNSLRSKPKLAELLKGLGVAPPMKLSEKKTETAKAKALVQL
                     DILRNPEASEKEQKKAWKTLAGLGIQSDTPDGVAAEIPELEVYDYAFAKTDLEFKQLA
                     DHPDPRVAALVTARMENNTSIAESRTRSLLNVQNPMPVPLQYAKAHTSRYTADEGINV
                     QNMPKRQGDKTLRHSITAPEGMEIGAADSSQVEARLLAYAAQEHSLLSIFQTKGDPYS
                     HMAADIYRVPEDDIKYWTKGEGAAEHEAHFSDPSHPSHAQAKRHTFMRNVGKETILGS
                     GYQMSGAKFGLRLRQQGVRLRPEPEQMMEWIRKMQASGKWPSEPGEQDVLMREYLQAF
                     HDAEARRINWLYRSKHQRIKGFWETCEWVLSRMVAGEQGHFGGPDGMLFYFDGQHKVF
                     GEHAPGVMLPDGYWIVYPGLRSFVEEETGFTKYTWKRMKDGRMVDDFIYGGSMTENLI
                     QGLAFAALKWQAVKVHQVVPVKMNVHDEWASVYPVALRDGVKRVYEMAMRAAPPWLKG
                     MPFDCEFKCGPDYGRC"
     gene            17569..18273
                     /locus_tag="ACHKUB_000382"
     CDS             17569..18273
                     /locus_tag="ACHKUB_000382"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFO6653457.1"
                     /translation="MGVKPLPITPSLLNTFLTCPRQYEAKYITKEVVFQQTEAAAYGD
                     RIHKAVESALKHGAALTPEADFMQPLVDWCRSMAARPGTEMFVEEKLAVTHALAPCSW
                     RGDKGMAPWQRGIADVFFIDHTNKLNIVVDWKTGKLKDDRTQQHILSLCASRKTGYSK
                     TLCMWVFVKEEDLITHVLNLEGLDPIAVHLQNVKAYEEACANNHFPAIRNGLCGKWCD
                     VLSCVHNGKNPAFNAK"
     gene            18282..18710
                     /locus_tag="ACHKUB_000383"
     CDS             18282..18710
                     /locus_tag="ACHKUB_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006705732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HFO6653458.1"
                     /translation="MTRYEDDFYTWTQEQRELLAAGRFDELDIEHLAEELDSMGASER
                     NALESRLIVLLMHMLKWQYQPGRRGRSWQFTIAEQRRSITRHLRNNPGLKGRLSEIFT
                     AAYGDARYRAEYETGLAIDTFPVDCPWTWEQVIDQEFMPQ"
     gene            18707..19135
                     /locus_tag="ACHKUB_000384"
     CDS             18707..19135
                     /locus_tag="ACHKUB_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HFO6653459.1"
                     /translation="MTRYEDDFYGWIQEQRALLATGRLSELDTVNLMEELAAVGRSEY
                     RELVSRLAVLMAHLLKWQYQPERQGRSWSLTIAEQRDALTDVLDDSPSLRARLPEAIQ
                     RAYKTAKISAERETGIPGRQFPVDCPWTWEQLMDEGYLPH"
     gene            19184..19666
                     /locus_tag="ACHKUB_000385"
     CDS             19184..19666
                     /locus_tag="ACHKUB_000385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653460.1"
                     /translation="MAKTPEGWVKDRIKEILEERKLHYFMPMTMGYGKSGEPDFDFCL
                     YGIYVKLEAKEDALRHRRGERITRGPNKGQVVRQGAPTILQQQTMCDIRASGGITLLI
                     DRHNYKGLAVFIDEVRALVQSGTLNLPFTGWMRQVITCAMRNDVYYGHVPEVICPVIQ
                     "
     gene            19648..21351
                     /locus_tag="ACHKUB_000386"
     CDS             19648..21351
                     /locus_tag="ACHKUB_000386"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFO6653461.1"
                     /translation="MSGYTMIKPGAWLMEDKTVLALKVGDAGRIANALPPGTAHVTAH
                     HGAQLVAVPYHMHTMRLLRNLGYDTGGMEVLRHTYPLPKVEGIYPLAKHQISQAAFFT
                     ENPRAFCTSTMRTGKTASAIVGARFLQEKGMATGVLVIATVSNLVGVWQKEINGLYPH
                     ATVNVLYGDKAKRRELLEQDADFYIINYDGVKLLRDELGDAVTSGRISVCIVDELTHY
                     ANTRTQLWEAAHWVINGARWEVIPGKTMQDGERSWKTKDRKKYLPSVKGVDYCWGLTG
                     TPGGPEMVYGEIKLIRPERIPFSFTAWRDTVCIKQGFKWIPREGYKKIIHMQMQPCIR
                     FDKKDIMDLPPVVMSGRDCDLSASQKKVYRQLQRDMLAMVGDATIKAVTKSTLVSKLL
                     QTAAGVVITDKGTEALDMQPRLDALEEIIKEATQKVVVFCAFTAVIDRLNDELTARGY
                     SVGVVDGRVTGAKRDAIFRTFQEDPNTYQVILCHPRTTAFGVELAAADTMVFFGPPLS
                     GDFVYQQAIERMSSLKQKAASCQIIHLSSTWEERNLFTSIHNGVSINEAINEMFTRKE
                     D"
     gene            21410..21805
                     /locus_tag="ACHKUB_000387"
     CDS             21410..21805
                     /locus_tag="ACHKUB_000387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653462.1"
                     /translation="MVTNLRKIREARRECNRVLKEIDEREQAITGSILTTMRASGLKT
                     ARFDGIGTVTVSTRDHAEIRDFNVLAAFMAQQCINAYNQGLPVTDAFGLLQRRANLST
                     AKELLDAGYTAETMGLAIVEKPSLSFSVK"
     gene            21817..23142
                     /locus_tag="ACHKUB_000388"
     CDS             21817..23142
                     /locus_tag="ACHKUB_000388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653463.1"
                     /translation="MSTNLPAMPAYIANMMPTLPQAMREQLAAAFAPALDSFGSAFNR
                     ISLKGSRFHLISGGQVTHTFNEAFTFVIVAISPEQHCVWYKNGYNGEEGVEPDAVWAQ
                     NEEPPANVPMSARVKDASGRNQYSMRRRFIVAPYMVNPQGNPYVDLDNLYVWDAGGMT
                     TFGDDVQLGSGPYPKAHSFSTYMNMCKSSGFYPCAILTTAIFDLRQSVPVARFLPVCS
                     QQGTPAILDMDTLQRVIEKSVDPTTQQLLDWRRGRSAEQQQQDVGTPVAQQPVQQTYQ
                     QTYQQTYQQPVQQSVQQQAPQQSYQPAPQQQTFQHAAQQQQVQQQSYQPAPQQPVLQE
                     NSTPVYQEPQSGTQSFMDEFANSPTLDGVVTQTTSPEAGHVDTVASDSTLAGVVKKMS
                     SAVDEANQTKQELVQEVVQSQPQQQQLPEQTDATAEDLVARMNDIMNTF"
     gene            23153..23422
                     /locus_tag="ACHKUB_000389"
     CDS             23153..23422
                     /locus_tag="ACHKUB_000389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653464.1"
                     /translation="MGRPGTQISALLQAADRGGIPVAKLAALLGVSRGMMYFYMKGSN
                     PSPDVAIRVVELTRRIHDALREGSLPLPERDFDAELKAVLNITQH"
     gene            23433..26579
                     /locus_tag="ACHKUB_000390"
     CDS             23433..26579
                     /locus_tag="ACHKUB_000390"
                     /inference="COORDINATES: protein motif:HMM:NF017826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO6653465.1"
                     /translation="MSTVLDFLHSVLPASGNFFCAAAIEPNSGKTKLYPVTSHEELAR
                     LVQKIANNRLDAYFALAAFEQTWHDDPKGRPGKNGAVKQVFRIQENAYAMRALWLDLD
                     VDPDDTKKYPSQGAAINGITEFCNRTGMPKPCLVSSGGGIHVYWCFTNDISAAQWNVL
                     SSALDAVLRHMGVPVDSTRTMDGASVLRPVGTINWKEKYGPGGAPVALISHGDIVHVD
                     VVVNALSRYMDTHGIVRTRKSPARQTGTAAHNSLQHLMNNPAFMAAMGHVMQEAKDKD
                     PVLITRECRQIREAGLEAEPTWHRMMSVMVCCFGGEIAARIISAYDKRRFNEQQFINK
                     FRYVESSSRGPASCQEFNRLCPGKCDTCPHFGKITSPAELGRTQRNIMPPATQTQTQT
                     QTQTQTQTQVASVTTAALGKPVTTPTPVSPQGPINLRSIGFEYIQGEGLYYLKPEKDE
                     AGNTTYFKQFIMSGYMFPVGKQEIHISFAQMQTIYIWKIISITGEVRQAQMLAEDQAT
                     PSALSKWCANNQLIAFPGADEVRNRYMRSYLHKVIDSIPSVRVADQFGWTEASLGRNG
                     SVRGFILGNTMLMPNMLPTQVAINASLEQFGRSRYTATGSLELWKQIPAACSRYNWLP
                     MQVALSLGFASVLMEFAVGSTQNIIVNFCGPSGSGKTTAMRAALSIWGNPERQMTRPQ
                     DTNNSQARIAGLSRSVPNGLDDVTKLEPKDLSDTVFSLSEGREKHRLEKNSALLQGQE
                     WKKVTIMTSNTSVLDALVPFAPDRDAEVKRVLELFMPRVPDRPAEEIAEMQRLERVMQ
                     QNYGQAGTAFVQWLMDTPGMIDRLPGLLDKMQMKFTEYQDERFWSATLAAVAVVVPMT
                     NAAGLTAFDVKQIISFMLEHRNSMRKQLLNSKVDGGSLLSDFLSDSLRNTLIVTDKDR
                     PVTVNGDMPLHQDAYVLRVPSMAGNLDVRIERDQQVAYVRCAAFHKWCKDRNTTPRHV
                     LSELAVSGVYDARVNGGKGYINMRLGRSISALAQIAPSRVYTFKLDKEGIDVDVLASK
                     TAKSEADDDEVHYQAEQG"
     gene            26548..26832
                     /locus_tag="ACHKUB_000391"
     CDS             26548..26832
                     /locus_tag="ACHKUB_000391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653466.1"
                     /translation="MKYIIRLNKVKDRKGEVVSVPPHAICFLYGLLPKDCVICGSPAH
                     AALPPDEAQHYLPVPCMGLAMRWEAIQDEFKAGIDNEDTRRLKEYLLAVG"
     gene            26923..27270
                     /locus_tag="ACHKUB_000392"
     CDS             26923..27270
                     /locus_tag="ACHKUB_000392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653467.1"
                     /translation="MIILQIDNTLLRNTYPGKYLTLAYKDIQSFMSRWDFYSVKGTGC
                     VFVGDNLRAPYKDDICSDVLSRLIKTHKWLAPALYEFNIFWVDVENEQRLCVLEMCGE
                     LSHISSHGKLRTS"
     gene            27450..27749
                     /locus_tag="ACHKUB_000393"
     CDS             27450..27749
                     /locus_tag="ACHKUB_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6653468.1"
                     /translation="MIGSFKDGPGGELARFYFTGVRGPGIPNQIEKALRRKLSLLDAA
                     SCETSLFVPPGNNYERLRGNLDGWSSIRVSLQWRLIFQWRDGYAYDVYLDPHTYR"
     gene            27760..28062
                     /locus_tag="ACHKUB_000394"
     CDS             27760..28062
                     /locus_tag="ACHKUB_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6653469.1"
                     /translation="MDTTTAEPTTVGEMLLEEFMKPMGITQIELAKALGVSRKVVGQI
                     INNSRRLSVEEAAHLAVLFGVDEGFWINLQAAHDRWESRQMLAKGRFRGGHVFCAA"
     gene            complement(28280..28516)
                     /locus_tag="ACHKUB_000395"
     CDS             complement(28280..28516)
                     /locus_tag="ACHKUB_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFO6653470.1"
                     /translation="MESYIRTRLDDTLKNQFETILQGCGLSVSAALRLFAENVVRNEG
                     LPFEITRKPSARLRESMRQTEELMAQGRPGGGVE"
     gene            complement(28604..29101)
                     /locus_tag="ACHKUB_000396"
     CDS             complement(28604..29101)
                     /locus_tag="ACHKUB_000396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653471.1"
                     /translation="MRNIIAGGVLIALAFTAGYFTGVTHEQLKVTDSANSIMQQGLNN
                     VTEDITVLTASLDAQRDRESKYAKDIQSDTARTEELLNRVMQHFDRVYDPPLPAREDR
                     ETPPSGDTCTPERAEARKLSGQLRETLEQYGNEARRADENTRLLNECIAQLALDRDVW
                     NQYHF"
     gene            complement(29094..29603)
                     /locus_tag="ACHKUB_000397"
     CDS             complement(29094..29603)
                     /locus_tag="ACHKUB_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6653472.1"
                     /translation="MKTSANGRKFIKAEEGIRYTLYDDATGRPIKRAVNARGNPTIGV
                     GHLVSRKDTRYDGVTLSQEEVDALLEEDLKEAERAINNNVHVPLTQNQFDALVSLVFN
                     VGPGTKGGKSGIITLTGGQPSTLLTLLNRHDYDGAAEQIMAWKYAAGKPILRARRMKE
                     RELFETPDA"
     gene            complement(29578..29883)
                     /locus_tag="ACHKUB_000398"
     CDS             complement(29578..29883)
                     /locus_tag="ACHKUB_000398"
                     /inference="COORDINATES: protein motif:HMM:NF017281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6653473.1"
                     /translation="MDYIIVMLASAANMSALMYMMLYTRNGATFKLHISSLAWLLMAA
                     TVFNLFRIWTDVGNICPMTRTEVIWQLLLFIPVILYKGNVSFLIPKEGYYENIRKRA"
     gene            complement(29888..30244)
                     /locus_tag="ACHKUB_000399"
     CDS             complement(29888..30244)
                     /locus_tag="ACHKUB_000399"
                     /inference="COORDINATES: protein motif:HMM:NF028241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HFO6653474.1"
                     /translation="MSTSVVQTVGNTPAVSSVVSVGLVALLPGVDMWTLLCACAGALL
                     FVLSAKEIALWERILYLPVASIGGYAAAAEIRQHFAMVSLPLAAFFGGLLIIGVGIRA
                     VKFVQNGDLSALLRRK"
     gene            complement(30257..30847)
                     /locus_tag="ACHKUB_000400"
     CDS             complement(30257..30847)
                     /locus_tag="ACHKUB_000400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653475.1"
                     /translation="MLKTPVFFSAALTRRLDKADRALPLAVQDEDRLAAILEEDDHTW
                     LTIRDDVGAEVVRITHACGGFVVDRGEDGTTPLNFPKGSCVRWEMTPAVVKDLICTHN
                     CCDDECPCDAVTAAGIALPEATKGVQWHGSAIFTGSTPMELAVAGAPAWVKVRKGANY
                     VTFSGVPAAPGKYTLSVAATNCDGKTAVQQGKLIVK"
     gene            complement(30841..31257)
                     /locus_tag="ACHKUB_000401"
     CDS             complement(30841..31257)
                     /locus_tag="ACHKUB_000401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653476.1"
                     /translation="MIIRLCEDTPKLAVRIAQPGCDGWQLMDTRVNIVIRPVVCVERG
                     TTHTYWDGCSFREEVIPDLVPTLRYPAFELDDEGRVVFYFDDKLWSLPPGRYQADIEI
                     DGVCVHTSMQIDLCNRPVAIEQVAVTAKASCGDASC"
     gene            complement(31254..38210)
                     /locus_tag="ACHKUB_000402"
     CDS             complement(31254..38210)
                     /locus_tag="ACHKUB_000402"
                     /inference="COORDINATES: protein motif:HMM:NF037529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO6653477.1"
                     /translation="MANNQNDLFNQFNPMTTTASSDLFAAPKDTTRDIYRQPSRYELQ
                     QRAAAQNEAVYNAALRDFQAVMEAPEWQNASLERRQEIYDDIKETQYQKFLSQYQDNE
                     DLYYKLQMIPELTLGANISSLKKQIDDANELADTWRGMQTSAHQVATGLGDSVPMAAI
                     KWQRDRLAGRTDAYAQRRIAELDEELARQVAEQAETLAKVKALDAQKSIARRNTELRR
                     EELQGENGEFLGSVGWALESPLDALSLAVEQTPTMVPVAAASAGGFLAAGPVGAGAAG
                     AAMGSLLSGGDAAGGASMDLLQRPMEEFQKMPEFQELIKQGVSAETARNLIIAGAAGS
                     AGPTGAAIGAATGVLGPGGMVGRTAGRLLTGTAARSAANSTITGAIGKGALKAAGGAG
                     VEGVEEGATQWASNTGVNTATGLNEDVWEGVGEAAALGVIAGGPMSTVSSTVDTVQGI
                     RAARAEKKAKAARGETDTTTHSEVVSPAEAAAAVSGDTSQASAPTTSEDSNKLRQALT
                     AYRDKLYETPGIPLTVEERNSLYDVAYAATKAGVSESAVNSMLEKVAIKRATPDNVSL
                     PDGFKAYAAARDAQTSATPATATATPTTTASAATTSTRPSWAGAESMEHVAPLAQAMM
                     SAYKRDSVVPQATYTPMQREPFDATVRSLPRTPRGLRVNTPETVYVRPDGKASASLSK
                     LQGKGNGRKDGNGTATTGAGSTGAVAGVAAGDGAATGTRMGDDIAASARAESAGLSGG
                     RGNTDTANAVYSESTGALGSGERAGTADTGRSDTTVSAASEAVSTGERGVAGETGQRA
                     TDGRGAESTGLRASGGDWSVGQKVVYRPRNGGKAREGVIAAINDDGTYDVDASVTRKG
                     KSVTERKAKVKAKQLSVLETAPVTEATPAAEVTTDPVVVEKAVVEAQAEALQASVNAV
                     MADETLPPAAVADVAEVQQALDEGDVAAAAELAAEARVKHGISALTYDELTDGRRDEL
                     IAAFRSLPDADRTQIREEWESLKKIGYYDDSGNIYMFTADAVIADMNEQANAEVPTFW
                     TKLSDGTKALIRKIRNALAAALVGITLWNAQPVADVHAATTGVVTQVQPVQGLSSTAS
                     VVRAWVQETKDNQGQKYIIADKVAGELYVMDSRGNVLATMPALYGSRKGDAAVPGQTP
                     AGIFMLQQRHDVGASLGGDVQQFAEHTDGSIWAIHRVLTANPKQMREARLKSATAADN
                     RISLGCINVPADMYDKYLKNGFQGKLYVIPEQKPLGEVFRDIQENVAVNDLMPGAKLP
                     ASLDSTDTMAFRSSTDNLAAEELTTGKLDTGATLNVLLATTDPESLSAMKSNDAADLG
                     AFAAIPAATEGIMRNARRRRNARLSESANDVEARETITGNQETPPVAAGGGNRRNDIP
                     DSWASTAWGKQETPKERVQSLWFNRQRWMIPVVTKLYDTQYRFLRWIHDNVVVPVGVE
                     LTDNKLASALKRAPGIRDAILADWTKLVEPSLARVAEIAEQKGMSVDDATRETMTWVT
                     MRHIPEANAELRRQMEAEIDEAIAEDRPAAEVNKAVKALEDFDAYQRGEPVEKVATAG
                     GWTSAQAIVTMRDIESRFSIEELESVAQPLYDALNVSVQARIKAGVLTQEQVDTWARF
                     KYYVPLYIHQESDGKNAFVGSSALNPLGEMKRHGATDGYAEHALMTVTESLNRAAASI
                     SIQPFKQHMNDMFVNADENHMVHGLMRVPVNEGTARDLRSKPGFYFTEHKEDGTTTKY
                     KFVFYDKYPDGDAIMEGLFPPEKDLNPILAVLATGTSAYARLLTKYRPTFAPVNWMRE
                     GAERAANIASRYITNEDGEVIGATDLQVSIRLAAANPAIVAALSNHLMTGGNTDNEYV
                     KAYRELEALGGLSTFSNILKQTEQDIRKDLRKMKGVRKYAKEMDNVVGHWNEMFGAAP
                     AVAAFMVLKSKGVSPERAAMFVLDSFNIHNHGTATQWLRSFQPFIVPAMEGSRNLIRS
                     LSTKRGMAVTAGRIAVGIALYTMLAALAPDDEMDKMTITELSRAIPVFNKDGSFFKIP
                     VAFGLPRVAWVTSAMLARSVREGGDFIADFSGATAALAEELMPLAGSYSGEHASNNPA
                     LAMFHAVMPELVRPFVEVATNASHFGTPIHIGNYREGERFASNSGKTMTAGVWKDAAA
                     ALRSTFGIDMYPESIRHIINAYGAGALSGLVNWLEKDSLYVTKGHMTTRQELGGFWTS
                     IGITALYDNGNKLSERRYWRLQDKANDLLMKYGVERSHPDNQRGAKAASAMRRMLAAG
                     ASYDEARFIRLAIETDSQRSKNNRTLNDKAKRLNRMNLSPDMLRSTFSQHLDKQNHLM
                     ERFIETSEGLNL"
     gene            complement(38215..38901)
                     /locus_tag="ACHKUB_000403"
     CDS             complement(38215..38901)
                     /locus_tag="ACHKUB_000403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO6653478.1"
                     /translation="MDDLQNPMVRGFFDLLANAEGTAGKGDNGYNVAFGGDTTVGYDA
                     HPGQYYTFTQTDGKQNRTSAAGRYQIIKPTYDSLAKRLGVTDFSPETQDRMALELIRE
                     NGALEDVKRGDYAAAINKLGGVWASLPSSTAPQPRHSMVELLGNQMPVQLVNQRGTPM
                     LAAPENDFERNHMMGFFGAGHSPAMQSLLTNALARVSEARNALASERPFFDDLPMDLD
                     NDLLDLIDRA"
     gene            complement(38903..39628)
                     /locus_tag="ACHKUB_000404"
     CDS             complement(38903..39628)
                     /locus_tag="ACHKUB_000404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653479.1"
                     /translation="MFFMPVDLFTPFIEGREKAIDRNWNDLNQANQVERGWLDNDAKQ
                     LSNWFKQDSYLDQLQQVRNQTDVSNMNRDLMAAYQPGNLAQAGFSSDFAIQQANAGYP
                     HIPELVNSWVTGLRGNAAEIAAKGTAQQKYSHNLMDALARLNVQKAIDANTLQPLKMA
                     AEKANSVAGINNALGTGTGATPSVTGGTTGIGIPDRYKQGSTANTTSPVSSTPIATNR
                     PTASDSYLDAMNRRYGIYGTWAD"
     gene            complement(39628..40038)
                     /locus_tag="ACHKUB_000405"
     CDS             complement(39628..40038)
                     /locus_tag="ACHKUB_000405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653480.1"
                     /translation="MKELKLMRKAGLHTVDSAPLFRCGYFVYAYDMEQWVAMCRILGV
                     DDAGDVGCSGVCQKFVRNTQQVYAIGVFDGGLDTLVHECAHAAFHFCDEIGITVSPDR
                     ANETYCYLLDWLFGQGVMGNRAAFRSCLEEPGGE"
     gene            complement(40042..41001)
                     /locus_tag="ACHKUB_000406"
     CDS             complement(40042..41001)
                     /locus_tag="ACHKUB_000406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653481.1"
                     /translation="MAACAMATGNWDTGMVKSQEVKTESIEKIENFLGMSKKNGGEDG
                     FWPEPQVTDTGVKSNAKWARLAAVAVATFNTLAMIDIAEKQLGIAEDYYKMARRDWDR
                     FREIFEPCERKEVAEACSAPLYNPKHDQQAADYMNEVDRTFGMAERRIHDLYGRYGIC
                     PDPSLNRDVALARSQVAGDSGNFAYRYEEGRQIARDDVRWNRRQQVLNRGRNIPTDAV
                     RYAEAAADAFNNQGKMLGNAAQGAMTALGYFSTRNETRYPQGRRDRRNSPYLVGQPEN
                     GVFGFISSSPAYIGGGGGMESVWYTGHDSIGSNIGSQNPGTGV"
     gene            complement(41016..41831)
                     /locus_tag="ACHKUB_000407"
     CDS             complement(41016..41831)
                     /locus_tag="ACHKUB_000407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653482.1"
                     /translation="MADEEELAPEGEEGAEGEEEVAASEPGKGVTDDGLTWSARIAQI
                     AAIAVAVINTKAAIDIARKQEKMAKKYLQMAKERQQYYYDVYVPCENTEIQEACSAEL
                     YAMHKDVQVGRAKASVRHELTGKTERELQCISRYCTGKRAAIVRDREVEEATALALSA
                     NFARRYEEKIKDARDDTRWARRAEALNRGRDIMARAQTFSGFAMGMFGRLGEQAANAA
                     AGAMGYLGYSMERREMQPREPLKRPQRMGISYPLPSPELEVIPPKPQPVKVLG"
     gene            complement(41833..42267)
                     /locus_tag="ACHKUB_000408"
     CDS             complement(41833..42267)
                     /locus_tag="ACHKUB_000408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653483.1"
                     /translation="MQYPKTADEMERFFTKVIGPLLVRHWAEEDQRTGQPHGLDVPAF
                     LAAWEQRGVMVIMAFEGDTPVGFMFVYVFRPLFLAGTVMQVERWYAEHDGVDKEMFDY
                     LSAIAPAMGINHIHAIACDARPVPEGIETDAADSYQMVKLVV"
     gene            complement(42293..43915)
                     /locus_tag="ACHKUB_000409"
     CDS             complement(42293..43915)
                     /locus_tag="ACHKUB_000409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653484.1"
                     /translation="MILKGFKGISPRTPEHMLGEGMATVARDVNLQHGTLRPWREPRF
                     VTQLPDTTLTVEAYGCCYFAWDSCVSVARWLPDVPRLYLTGRSPYPEVAAIGKDCTLD
                     YARLGVPAPDTTPLVAYITVPDKNIETAFRTYVFTYVNWLSEESAPSYPGTELAVNDG
                     QSVTVSGWEAQPAEYRVQKVRIYRRTTGLRSGAEEEQNIITEYLLVDEIDITQKTYVD
                     TRKDMDLGWQLPTREVREPPAGLRGILAIDGTSVLAGFVGNRLLFTKNGQPWNWPLAL
                     EMTLDDNIVHIAQDDGNLFVSTTGRSYVVDASLVCDERPCRSVVQADYPMPDIGCGYA
                     HSAIMTPFGMVYASTDGLVLLNQSQPTRLLTEGVLAADDWIKLRPETTRLAYYKGYLF
                     CVTDAVSFMLLLDTQSYSAAAQVAAMSTFSDTPVDMVLSDSGELLMLDDEGKLHQWNA
                     GDKLRPYLWVSGVIMTGFQSWLPVAQIGVDGTVTYMIKSGPHKVYSRPVAHNRPFRTR
                     RLMRNTEHRIILEGTGEVRWLRLGATMQEAVK"
     gene            complement(43912..45183)
                     /locus_tag="ACHKUB_000410"
     CDS             complement(43912..45183)
                     /locus_tag="ACHKUB_000410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653485.1"
                     /translation="MNKCRKTPKNARCAPEPVEAVPPLCDPFSVCLSFGRTLSYDGAC
                     VRLNGQPVIPDGWYGEVHVVNGCIVDAREAPVPLYTPPPCAPAATPCGEGGGEGGVTL
                     SPDAANLSRLIGNQLMTKLYLGQNEGVTVTGDGTQNNPLTIRLVAGDGDGIFMTSGSP
                     DALTISGSGEQADPFILKLTNSPLGAGTFGALELDSLGRVVGYNEDAGGDALRQLIDG
                     NGTKGVTVGPGVDRIDLEEVEGVKGSYTMGGYSLSVDDTGRVEGVKRGITLTAGTYTL
                     GNYSVTINAYGSITKLEKAAVPVEPNVFVAQYAAGEPSQAAVMSFTTEVPGRLHIEYI
                     GYLGTATAQTPGLAENVTGTAIAVDGSDVPRVLTTLMPSGTPGTMVSTGVRGITPGII
                     PAGPHTVAVRGPEIMGQHAAILKCTVVGVGT"
     gene            complement(45180..45818)
                     /locus_tag="ACHKUB_000411"
     CDS             complement(45180..45818)
                     /locus_tag="ACHKUB_000411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653486.1"
                     /translation="MQTVVFEAYPKVPLDAFLPELSLEIPELSDDILAHYVRNAAIEF
                     AERSLALQREITICLQSCVPDYILEAPDCMRIVSLHKVKKNGDRAYADCAGFCQFDDL
                     YVVWKQPNELWVKPVPVEPQDITVIVSVAPHHDACELDEILLTRYKEAVLAGARAQLY
                     GLQRRPWTSLVSATAQRKEFDRRIAAAGTDRLLQGRTGRVRMQTVFNGRRTR"
     gene            complement(45799..46491)
                     /locus_tag="ACHKUB_000412"
     CDS             complement(45799..46491)
                     /locus_tag="ACHKUB_000412"
                     /inference="COORDINATES: protein motif:HMM:NF042497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO6653487.1"
                     /translation="MITQALVLSISGDLNDNEDGYHNQTWSEQQIREWVAEGVNLVFD
                     KRPDLFMEKKVIPVTPCSVIQDTCDCGAIRRVLGHATKDGRLLSVMRKQGLESSLQWR
                     GTSCRRATGKYKPTSYALDAMTDALYVWPEMPPHEEAYILVECAVRPDADSIQSEVDD
                     AYVTAVKQWVLWRARSIDAEVSPAAGNAAQMHYKAFFDVLGVAEKATVVWHRSEKDAS
                     EPKGWRNANGSI"
     gene            complement(46491..47114)
                     /locus_tag="ACHKUB_000413"
     CDS             complement(46491..47114)
                     /locus_tag="ACHKUB_000413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653488.1"
                     /translation="MVTAVTHDEADIRNEGILAERQAELDTLATKMPKPIPGRPPYLM
                     EKATGIIHPFSDGLAQRSDLVVACYNLQGSMNPADANPDYDPQGITIVEERQRRLRDL
                     PPGNVAEMKASLSDEIAAIRAKMLEEIELERQAMRDDMAVEYESFKKELAAMRGDATT
                     AKAAKKKDKSKASEKAVSEPETPVTDAAITGTGETFDTALDDALKDS"
     gene            complement(47116..47331)
                     /locus_tag="ACHKUB_000414"
     CDS             complement(47116..47331)
                     /locus_tag="ACHKUB_000414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653489.1"
                     /translation="MKKIGLNGDATKTPKGKPSTKLKAGKLAHGGKGRDASAITKLKA
                     GKLGSTSKGRGPEAITKLRAPKGKVGI"
     gene            complement(47390..47965)
                     /locus_tag="ACHKUB_000415"
     CDS             complement(47390..47965)
                     /locus_tag="ACHKUB_000415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653490.1"
                     /translation="MADAKLFIGGTPNPNYPNCNGAYEFAEPHARRIAFEYANGYFTL
                     GWPLQPSEKNYQFQELMNAETGLNDHIMMYVVPEQHLLTGVLFEVLEGDARMAGAALR
                     PSGMLYDHTTKQYTPDTVLDKLFTNTAITAPFEKYGHLAEPYYVPKGKTLVLTLRVNA
                     VPTDKDVKLHTTTAKAQLVAKVQGFDVPTEV"
     gene            complement(47968..48915)
                     /locus_tag="ACHKUB_000416"
     CDS             complement(47968..48915)
                     /locus_tag="ACHKUB_000416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653491.1"
                     /translation="MYKSASGYGGLEATPLARIGYYDEIIAQAWEKDFLPEITNSEIN
                     ERITQCNQIVQFTRQPKVGPWRPYDKNQPLIANQLTPESFCLEICNAAYQDIKFDKLD
                     IARACKQWDSWEASFLDSTYQSLTDMWRSWVLNAMVLEADPGNKGNKAGIRHNISLGQ
                     SGAPNMVTPNNITRQFARLRQVLRDRQRWEEDKMFVIVPTALNEVLSVSPYADAGKMG
                     DCKPCSQNIDGFMPHKLFGFNPIESSHVPAFVDETGELTYYIIAGHRDAFGFAADIIE
                     GRLVEPSRNFGVEYQMLAAWGGKAIYPDALAVGYWAFGD"
     gene            complement(48938..49909)
                     /locus_tag="ACHKUB_000417"
     CDS             complement(48938..49909)
                     /locus_tag="ACHKUB_000417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653492.1"
                     /translation="MAYSMQPRRHHAAAAVQARREQEAEEAKKNESGQEQSQASAPEV
                     SAQVTSESGAPTNAANILATNSDRVQELEREVTTLRERLSEQSNELAAAQSRALELPE
                     DVQKELAELRKYREDLEMQQVLDVDQEFETLDREAAAELSAKVLRPALERLEKRYEDK
                     LRITHEELSRIQKERQERDASMTEAQKSSERSRVNAAILGAHPDFEKVLQSEQFAQFS
                     SRPAWKGSRVTLGQLMRDEYNNGNADFVIEAMNDFKGGKPSLADIASVSSATTASIPA
                     SGVDEQQYTDEDVSLWNRQLTTGQITREEYKELRAKYKAQKVAASAA"
     gene            complement(49920..50162)
                     /locus_tag="ACHKUB_000418"
     CDS             complement(49920..50162)
                     /locus_tag="ACHKUB_000418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653493.1"
                     /translation="MITVETPQADLLMTLPRNEAAYAALVQAVETHAANRRALLQQVA
                     LSALVSPAARDNGLRLMGEVDALAEVLDLLKRLTEI"
     gene            complement(50174..50332)
                     /locus_tag="ACHKUB_000419"
     CDS             complement(50174..50332)
                     /locus_tag="ACHKUB_000419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653494.1"
                     /translation="MACASCGGKSRRPLRTTQGGRPVNQTSTRTPQSSGSRPGIRDRI
                     AGLRWNSK"
     gene            complement(50374..51666)
                     /locus_tag="ACHKUB_000420"
     CDS             complement(50374..51666)
                     /locus_tag="ACHKUB_000420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653495.1"
                     /translation="MSNNCCTSSLVEGGTLLEPRIINGELQSPSVKGSITLDEPALLA
                     LSAQLCAVLSDCIEREIHGGSFNDVTLRDAELHSAKLITAALEGAVTLNDAAKKSIAD
                     AVGPELTTHVLEIVSENKIADAKLSGASLSGGITLDKSVSQAIADAVGPLLQKHIIDA
                     VTDNGLDGIQLRDVDIENARLSGTLELVGGAKTLLHAALSDLIKKQVDTLISSALTSW
                     EITGNNLVNISAENLSASNTRLSGTTSISGQVPLDTAAHDHLVKQLTNSIREIVDAAI
                     VADKDVLAGVFQDCDGAPRAPGVRIPSCQDVTDAINQAIASLPALDVISGFTYDEDTH
                     TLKLTTKLNNDGGPQSWEVNLDSIGGQVVTDGTTITGTGVTGQPLKLVIAETTKVKAV
                     TKGAELPTAIHGGRTALLGQPDKWIDIGGYLIPAFNKP"
     gene            complement(51666..52115)
                     /locus_tag="ACHKUB_000421"
     CDS             complement(51666..52115)
                     /locus_tag="ACHKUB_000421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653496.1"
                     /translation="MNTDILFDDASTSEYSRAFVVSPGCTAIISAWGFAQYIKAPEEG
                     QPKNIPQLAIVQKMAFDKGIFPNGSACAADEGLISVPKYSYVEDVTQCGLWALSACNN
                     TVALSVPGTYRLQLNDAAAVGTVFISLTRYSQQELGYIPYGMYLGVE"
     gene            complement(52117..52608)
                     /locus_tag="ACHKUB_000422"
     CDS             complement(52117..52608)
                     /locus_tag="ACHKUB_000422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653497.1"
                     /translation="MGIDTGGLYDDRGAHAAEKSVTGKHMVLFDPTSTYAQSALISVR
                     PGSSVLLVGYNIPEDAVFTVEGVSVGTSPAPGGQGCCRNGSVLGNSIVPGSAPNILFR
                     SPVKLGGKPWQLTKDNDRLLIALPGEYLLVLNDTQHLGDVQVELIPVPGYQQLPFAYM
                     AGV"
     gene            complement(52620..52877)
                     /locus_tag="ACHKUB_000423"
     CDS             complement(52620..52877)
                     /locus_tag="ACHKUB_000423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653498.1"
                     /translation="MSDLKEKAEELKEKVASEAQELQEEASTLRGKLEAWIHKINPCN
                     APETSLRWRIASTLFPDCWCCAGLRGFIYGVGATVFVWWLV"
     gene            complement(52870..53163)
                     /locus_tag="ACHKUB_000424"
     CDS             complement(52870..53163)
                     /locus_tag="ACHKUB_000424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653499.1"
                     /translation="MINGVAPYVGAQVYDIQQGYGRITQVEPDMSFVVDLGGGRVLRY
                     SKGGYVGNSRRVYWKNPVIVEPVDNDRTWDTFTTMAKSLRNMLNDGIKAVPNE"
     gene            complement(53163..55091)
                     /locus_tag="ACHKUB_000425"
     CDS             complement(53163..55091)
                     /locus_tag="ACHKUB_000425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653500.1"
                     /translation="MREEAQEVEVPKKAHDPLANVTAQRWNAAVQWRAREQIGGKSLR
                     TVLRECYEQREGILSPEDAEIADELGIDIYVNISALKVSALTSWLRDLLLNTPELPFV
                     ISPTPIPDLSEKARMEVLNKVKMELYAAGGFQGDLLSLIRSVKFQQLEKERQYAEVAC
                     NNMSRLIKDQCMEGGFQEAFLKTITDFATYPFAVFHGPVPTMVTQMEWSGSSLKPKDK
                     IQFQFRPISVFDFYWSPDSRNAQEGTGIFIREQVTKQDLYKCLGMKSYIKANVQKVLE
                     ATISKTQNLKWMSRNPEQPDTIGSGWGNGETLDVLRHYGMFSGKELKKYGITGIDDDQ
                     FYDACISMVGQYTIQAFILPNPHVGIRPVYTASFETAADRIPGFSICQKLRDVERAYM
                     NTLRFLMTNEGLSAGPFGEVDYSRIQRYMNPEDIGRITALTLHPVDPDMSAGGHPAYK
                     LQHIPSNMGAYLNCAQFFMDLADRVTQIPAAIHGEPVGTGANRTFRGMAMLYGNAIKP
                     IQSAVGNMDVGIFRPLGTLMYNYNMQYSDDESVKGDAKIIAQGATGLISKEVAKQNAF
                     DTLQLVATMGSAAQGAINPGIMKWAVENALKASGVPVDELEAIPAAPPVMPQQGVPGP
                     QQQGGGEMMPQSGTAEGA"
     gene            complement(55091..56581)
                     /locus_tag="ACHKUB_000426"
     CDS             complement(55091..56581)
                     /locus_tag="ACHKUB_000426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653501.1"
                     /translation="MRQHLYEAYPTASKFHAAWKVKKIRAICGPFGSGKSVTCVNDLL
                     YIAIRQKPAPDGVRYTRFGVIRATYPNLRTTTKKTLEQWLIHEAGGIKETAPMEGHYR
                     FRLQHDGTLVDMELILLAVSDDESIKKLRSTDFTAIWINEATEVPASVFTTATERIGR
                     YPSGERGACSWRGVIMDYNMPPRGHWLLNLFASERDDYALFMQPPAAFKHESDDGMIS
                     YEVNPEAENLVPLGGNDGFEGGIRYYQSQIDSKMLVGDYDGIDQLLCLKVVDDRSGKP
                     VWPRFSRAIHVAKHVILPQPGLETMISIDTSGIHPAALFWQFIGVKWVILDELYGEET
                     GFEDFVYGGLLPLVQSRYKESSGILAICDPANARNALTATTPVATLNEAHIRAQVAGT
                     NNTKSRIEAVNRLLNKDVGGLLISPNCERVIASCAGGYKFGKMQIRGTVDVAYTANPV
                     KNEHSHPADALQYGALYALRYDESSGDVSAVKRAFRDRLRKRRRIM"
     gene            complement(56578..57123)
                     /locus_tag="ACHKUB_000427"
     CDS             complement(56578..57123)
                     /locus_tag="ACHKUB_000427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653502.1"
                     /translation="MSEKDNRVVEVSQRKDLSRLRPHWPCIAQDLIMCRVPDGTGNGM
                     LLNPEVICRNYGMTMEECLALMKIPAFLRMMHDFKKRIDAMGDTASVTLRAQMITADL
                     MERLYKDVVEGADVSQIRERRETLRLMAQLGQLDPATNGTNKRRDDGGGSAASVVSVS
                     INMGPGGAVYDVSADVGEGGA"
     gene            complement(57413..59050)
                     /locus_tag="ACHKUB_000428"
     CDS             complement(57413..59050)
                     /locus_tag="ACHKUB_000428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653503.1"
                     /translation="MSDKTTVKVVVYKDEAHKLAGTGDVIDASLIPLSQTPGNILYNE
                     GGALAARFDVRGGFGIDTDMAFNGDADHGVLKVGLNISKDNDNLLFDPDTMTSDTELI
                     NESGTLKASFYPDAERSDLTFEGHGSKASPIVMKLKDSALPTINAAHMPSEGVETSGN
                     PIAPSEVGVVKTPHLATVNLLEEHEPKKWSAHLYVTREQSVKVQGLGVKTDPLVIALN
                     KAESTPEKPNAIEITDKGVLVDRYKLRDFENKTVQFMVPETPKVITPKMGTKGYEHMR
                     EELLVLNGQNTDYTVDLSAIEGEQEYSKHIRLVYYAVMLDGQDKEGAQRRGYSTLKFV
                     TKDGRIITPYGKTVRANEFVLTSKEHVAAVFDIYFGDQKRVMVNCPVQNVADTQVFVK
                     EFTTAFNNVGDSTGVLRVPLDALPLEGLRCAYAIDIDVSCVLTEFNEDTEVSLPDFRI
                     RANNQDVTGFPRVVRLTMANQTATFTHVCAAELVSKGSKGSLVPVEVVTGTPSFKGGT
                     TGKAVIRLKVMVRRINNKVSDINVLNATKDVDITVGS"
BASE COUNT        14379 a        15924 c        15926 g        14556 t
ORIGIN      
        1 ttcaggtaca cccccccccc tagaggaaat tttcgacttg aatttttcaa ggtggctaac
       61 ccccctaaaa atcttttaat gtccgaaaac gtgctacggt gctacaaagt ataaaattct
      121 gcatgttttt ctcttgtatt tcatagagtt acccgatttg cccccgaaaa aacttgctac
      181 aggattttac tcgggcttac tcgcgtgcta caggcctcta accagtacaa aaagtgcaca
      241 gaaccagttc atttcctaac caataccccg atttccgcta tttttcgtca gatttgccta
      301 gtgaatcgcc ctgttagttg tacgcaatag accgttttgg caggatatca tctaagtttt
      361 tagccttagt tttcagggat tcactagtat ccatgtgtct acggcagaat cggattgagc
      421 accaacactt gactactcca cctgttttgt gtttaatagg ccccaagcag gacgacgcag
      481 cgacaccgcg aagcctgccc cgcttcttac aaagcgttgc tctttaacaa tctggtaagg
      541 cgctacgtag tgagcaaggt cactgagaca aaccagcgtt cactacgaat cagggagaac
      601 ctctataagg tggcccgttc agttaaccgc tgagcgggtt taccggacca gccgcgcgag
      661 gcatctgctg actgcggcgg gtgcggtaaa cacaccatac agaggaaacc acgatgatta
      721 aacaggcatg ggaatacatg gatgccgcta cggttataaa gaatgccgca ggtatcagag
      781 cagatgaccg cgcggcactt cttgtaacag ccgacgggcg gaccgttcag ggtctggcta
      841 agcagttgcc ggaagaactg gcgggcaagc tggaagacct ggcgaaatcc atgatgccgc
      901 atacacagcc ggaacgtcat tacacaaatg acgcagaggt gcaacgcctg ttgcagtcgc
      961 tgcgtatgtg tgtggtacag gagtttatcc gtaccagtcg cggcgagttc gacgcggcgg
     1021 cgatatacag caaaggcaag ctggcagctg cgcgggtact tactgaggtg cacggcatac
     1081 ctgctgcaca ggttgcggaa aaatgcgcat tcgcaatcag tgattggcgc tgggaggtaa
     1141 gccgcgcaat ccgcgaggct aaccgcaatg ggtaacagga cggtgatgtt ttgtggcgtg
     1201 agttacacgg ttccggcggc gttcggaacc ttatcacgcg atgaactgga gtatgccatc
     1261 acgctgatga gacgtggcga ttttgacacc ttacgcgagg cgtgcgcgtg ggtagcgggg
     1321 gagagtgacg aatgacagtt attacatggg atgaactgga agaactctgg gaaatgtggg
     1381 gcacggatta cgccgcatac tggcatagcc tgggcagaga cgaagaacga ccgtcatttt
     1441 ttgagtatgt gcaggcgttg ggatacatac cacaggacgc gatacttgcg gactagacag
     1501 cgaattattg ccgctcatgg gggctggata gcgtgaacgc tgtccggcct gctcacacct
     1561 tgcaggtgtt gggggcggta atggtgggct gtttatcagc gaactaatcc acacaccata
     1621 caggagtaag aacgatgact actgaaatca ccattaccgc gccgtcagta tatgttggaa
     1681 cgtatgcgaa gtacaacaac ggcagcattg ccgggaaatg gatcaacctg ctggatgatg
     1741 tgacagaccg cgatgatttc tacgagaaga ttgcggagct gcatagcgac gaggcagatc
     1801 ccgaatttat gttccaggac tatgagaaca tgccgcgcgg cattgtctcg gaaacgtcga
     1861 ttaactggaa ctatatcgac ggattgcgcc gggtgctgga agagaataac gaagagtggg
     1921 cggaggctta cgcgttgttt gttgagaatt tccacgatac ggacgtagag aacttttacg
     1981 agtcatactg cggaacggca gacagtgagg cagattacgc cgagcagctg cttgacgaca
     2041 cgggcgagct gaatgccatt ccgagaaacc tgcgttacta cttcgactat gagaagtttg
     2101 cgcgtgattt gttcatcaat gactacacgt ttattgatgg gcacgtgttc cgcgactact
     2161 gacaggccgt caattattgc cgctgtattc ggaagcatac cgggcacggc ggcaatggtg
     2221 aacgacctta cgcatgagga gaatacgatg aataaatata ttgaaatgcc gtcgcatatg
     2281 ctgcgcgcgg cgctggtttg tgtggatgat aattcgtcgc gtctggtgtt taccggggtg
     2341 catattacgc cggaaattat ggaggcatcg aacggtcatg tggcggtgcg catgacgcac
     2401 ggctgtcagt gtgaccgcga catcactgtt atttttaagg ggaaaatccc ccgtgcggca
     2461 gaggtgacgc gcattcatcc cggagatacg ccgatagcgg agcatattaa taaaaagggc
     2521 gacgtcatcg ggatgacggc gtgccaggta cttgatgcag agtggccctc ggcacgactg
     2581 gcgcagcatt tcccggataa gaccgacaca tcgacggtag cgacgctgga tacgcgctat
     2641 ctgacgttcc cggataagat gttcggcacc gggcaggggc gcaaacagat tggctgcgca
     2701 gtgtgcccgg ccccggcagg atgcgctatg cgttttcgct ttgaccgggc gacaacgaaa
     2761 ctgttcggta atccggtatt tattgttatg ccgatacggt atgacggaga aaccgggctg
     2821 gagtaggact tatacagggg gttaattgca accacttgcc gcgcgtaggt ggttgtggtg
     2881 aacaaccttg catacaaggg gaactgataa gggggaacca tgacagactt taatgagcaa
     2941 ccgccagtag taacgcgaag tggcacggtg cacgcgccga acctgataca caagcgcgac
     3001 ggctggcacc tgagttattg cggcagcagt gcggcttacg gttgtaagac tacggcgctg
     3061 gttatagata accgggtttt ctttgtgctt aagggcgacc atcgccggga gtggatgact
     3121 gcgcggacgt tgtgggaggc gctgcaatac tttgtagcgc atgatgatca gattcatccc
     3181 gcgagtgaac accgcatgtc gttagggtta gatgtggata gcttcggact gatgcccacg
     3241 cttcgtacgg cgctcacgtc ggagcaattc aatttgctgc gggaatactt tgcagaactg
     3301 taaagcgcgt tactggtacg cgcctgtgtc ttcgttaatc gtggagtaac ttaacctgta
     3361 atattatttc tcgtgtctgg tatgcttaat tgcaggataa ccggacgggg ggatatatga
     3421 cgggcagatc atcggcgtgg ctggttgtac atacggcgac ggggtacgca gaccgggccg
     3481 catatacacg ggatgagatg gcggcgcttg atatcatggc tgatgacagt ggcggcgagt
     3541 tgtaccttgc cagggtgaaa gaccgtgtac acgcggaggc ggtgcttgca tggtgtagcc
     3601 tgctggcgtt cggtacgtgg tcacttgtgc gcacggatat ttataagcgt gatggcctgt
     3661 tcgttgagtt atggcccgac accgggaaat ttatcgggta cgctggcgca cgggcggcag
     3721 ctgtcgccct gaatgccgca caccaggacg agggctttgt gtgggagtta ataccggaac
     3781 atcctgacgt tcgtgacgga ctggcagcac tgcggcggta tgtggtggcg atacaggcca
     3841 gtaaggacct tagccgatgg ccctgctttg gcgacgatat aagaccatac acgcgtcgac
     3901 gtggcggcag gtgtgtcact gtatggcgtg cagtctgagt gtatacaaga ggacgatgac
     3961 gtattcactt gatgaacttc tggacggcat cacgccggag aatatccacg aacggcagga
     4021 cttcgggccg ccagtgggga aagagttgtt ataacagtaa cgggggatag cttaatgact
     4081 catgaaatac tggcatatac ggcggcaagt atcagcgact tgcagcgtaa tccggtggag
     4141 acagttgcgg cgggtgacgg tggtgctgtc gttattttta acaacaacga gccagcattc
     4201 tattgtgtgc cgcctgaact ctacgcatat tatcgtgagc ttgcggagaa tgaagagcta
     4261 aacgccatag ctgacgaacg ggcgaacgag acggacagca tcagggtgtc tttagatgac
     4321 ttatgaaatc aggttcttgc cccgtgcgct gaaagaatgg agcaagctgg ggcatacagt
     4381 gcgggagcag tttaaacgca agcttgcaga gaggcaggat aacccacggg tgcaggctga
     4441 cgcattgcgc ggcaagccag accgctacaa gattaaattg cggtcatccg gttatcgtct
     4501 gatttatgag gtgcgtgata ctgagattgt gctactggtc atagctgttg gtaagcgcga
     4561 aggtgacctg gtgtatgacc ttgcggataa gcgttaactc tgtgtggcgt ttcgtgactg
     4621 cggcgaactg aaacaacggc gggggtggag gttggtaaca tagcacagga tttaagccac
     4681 taaaccacac gcgtggcgcg ctgaacaggg tatagtggta tggtcatatg gcgtaggcat
     4741 taaatctcgc cagcatgcat ctgagagcgt tttacgcagg ggcactacca gcactgacta
     4801 aaagcctgac gttatgcatc tgtgcgcggg tgtataacgt cacgctgtaa ggtgatgacg
     4861 actgggataa caacaccggg cgactgcggc ccggcaaaaa tcggcggggg tgggtggcgg
     4921 catctcataa ccctttcttt ttcttaaaca atcctttcaa tatactggag acggaccatg
     4981 aaatactgga ctgtgaccac gcacgacggt cgtgtggggg tcgtttatgc tgaacaggtt
     5041 atccctgata atgtcgtcac tatggtgagt gcagaatggg caggtacgga ggtcgttcag
     5101 aacgacggaa tgaaagcctc gctggcggta ctgtacgtat tacgcgatgg catcgtgcgt
     5161 agtgcgcaga taacacctgt gatagcgggg ggagcgccgc ttgatgacct gtaccgacgt
     5221 tccacgttcg tgcagaagta taacggtgcg tggcgtgacg catcgggaga ctgggagctg
     5281 tcactgaggg acaggagtgc ggtgcagccg tgggataact caatggcccg gatggaggct
     5341 gcaagcctga tgctgctgga cgaggctgag gggcgggggg cgtggcctgc gctgataaat
     5401 gcgataacgt gcccccgtta tgcgccgaat atcagtggtg tcatgcggca gttgccagca
     5461 gggacgcggg tgtgggacac acggtatcag cgtgtggttg ttgcagggtg aggggtataa
     5521 aaagccccca tgacaggagt acatggggga gaatacgtta aaccagatat gttaaggagt
     5581 attagtaaca ggatataacc aatgcagtat gacgttagcg cgcattgcgc atgggtgcaa
     5641 gtggtttttg aatggcgggg cgacggggga aattcgtcgc gccgcaccat gattagcgga
     5701 gtttatatgc cccgtgcggc atgacgttac ggagtgacgt tataccggac gcaggtcaga
     5761 gactggcaca gacaggtact gagtgcaaca actttatgtg ggggtgggta tgttcggaac
     5821 ggtggaagag tgtgtggccc ggctgtcggc acgggcaccg ggtgagcggg tgatggtgac
     5881 tgtgtggaca gttgaagatg tatgggaaga gcgcccggac ctgacggcgg agcaggcaga
     5941 agatgtgctg cgggttgtta accgccgtta cgggacgggt gactggagta cgctggcaga
     6001 aatagcgacg gatttatttg gtccgcaggt ggcggagtaa ggggacagtt atggcggtta
     6061 tacagtatgt gccgttcagt aaggcggtga aggtgatgga gaaagccggg gtgacagtcc
     6121 gcgcggcaga ggatttcctt gccgcatgtg acgaggctaa gggtgtgccg atggatgagc
     6181 tggatgcgtt tatacatggg gaagcgctgg agcaccgggc gttcctgaca gatgaggagt
     6241 gagataatgc gcacagaaaa acagatacgt tcgtggcgtg atttacagat agcgtatttc
     6301 gcgcaccatc ctgtaagtgg atcatacacg gcaccggaaa cgccgagcgg atggtgcagc
     6361 cgtatcggca cgcacaaggc gcggatggag tacaggggga cacgggatga cggctgtccg
     6421 tgccgcacag tgtttaccgt ggagtcgacg cgctggaaca gtaagtattc cgcgtgcaac
     6481 gtgagcgtgg cgatcgagat gtcgccggat tgtgggcatg aagatacctg tcgtcaccgg
     6541 gagtcatgcc accgccgtga gatggtcagt attaagcacg atggtacgcg tgttatgcgt
     6601 gcgatacgtg acgagataga tgcgtggagc ggtagcggga caatcactaa atcgaaagtg
     6661 ttataagggg gtattatggt attgccagaa accatatcac gtgacgcggc gcagatgctg
     6721 gcactggaga tacacaggga gaaggagctg atatacagaa acataggcac cgattaccac
     6781 accctggctg aatatgccgt cagtctggag cgtgagcaga gttatcgcgc cgcgtattac
     6841 gcatgggcag ctgcactggc ccgtgcccgt ggggtaaatg tggcatggtg tgaggcccgt
     6901 atgaaatatt gtggttttaa tggtggatta atttcccgcg cacagtgcgc ggcacaatga
     6961 caggagaaaa cagaatgtat aaatgtcgtg aagtttctgt cccggcggag ttactggaga
     7021 cggcgaatgc cgagatagca gctgctttag gtacgctgta tgaggtgtgc aagaagtata
     7081 acctgcctat gactgcgacg gttattgata cgcagtttca ggatgcggac gggggctggc
     7141 acacaggtat gtcgagcagc cgttatgcag cgggtgactg tattactaca gtgattcagg
     7201 cgtgtgtcag tgaagggctt tacacacagg ctatgcagtt actggctgaa attgtggtcc
     7261 aggaagcgct tgatgacggg gctgaaaagc cagtatgcca ttaaggggga taatattatg
     7321 gcagcactaa aagatgagga tattgtggag tacggactgg cagagtacca ggatatttac
     7381 acggctgcgg aggcgttact ggataagggc aaagatgccg gggttccggc gttgcttttc
     7441 ctggccccgg ttacggcacc gggtagatgt acgctgtctg ccgctggtgt ttgccttgat
     7501 aagccacgga cacttcggtc attcgatgcc gcggcgcggt cttatgcgga gatacttaaa
     7561 attatggaga gtcacgagga ctcagtggca tcaccgacaa agcactagtg gtattacctg
     7621 gcggctatgt gccgccacaa taatattatg ggggagctat gaataacagg aaagcgcgtc
     7681 tgcttctgac gcgactgatg ccgctggctg cgaatagttt atgggtacga gtcagtaatc
     7741 gtcggattgt gcaactgaca atatcaggtc gttttcgtgc tggatatttg tgcaaacgta
     7801 gcgcggcaca aaatcggtgg aaaaatcatg tgaggtataa ctgatgcgtg tttttatttc
     7861 tggtccgatg acagggtatg caggttttaa ccgggcagca tttaacgatg cggagcgtat
     7921 gctgaaatca gagggatgta ctgtgttgaa tcctgccgtg cttattgacg gtcttacaca
     7981 ggcacagtat atggatatct gtctggcgat ggtgcgttgc gcggacactg tgttcatgtt
     8041 gcagggatgg gaggcctccg caggtgcccg tactgagcat gcactggcgg aaaagctggg
     8101 acaccgcatt ttatatcagg agtgcggtta tgtggctgag cgagttcctg caatacgtgt
     8161 tggtgcaggt gacgttgtga ctggcgctgg tcagttgtgt gaggttgtta aaactgacgt
     8221 tgacgctggt cgtgacacgg tgacattaca tttctttggt ggcggagtgt atcagtgtgc
     8281 gtcgagttac ccaatggatg ttgtttggat atctgacgct attgtaggcg gtacagcatg
     8341 acgattgtaa aaactcatac cggcactgtg gtcaccagag atgggcctca agtaaaaaaa
     8401 ctgcaccaga cagaaaggat gtgggtcgtc ggcaaaaacg agttctacca caaagaaact
     8461 ggacgccgcc attttgcaga aaatacgcgt cgccgactgc tgttagacac cattaagctt
     8521 atcgaggtgc aggaccatta cggcaaactg ggggtttgac catgatgact tttaccagag
     8581 aacagttagc taccttgcgc gaggcatcgg aggccgcgaa ggagaagacg acgtggcaga
     8641 gcctacaggc ttttcgtgct gcggctacgc cggaggttgt actggcattg cttgatgtat
     8701 tggatgcatc gcgggaagca gagccagtgg cgtggaaggc ggtgtttact caagttgacc
     8761 aggaatctaa tacgttcatt gctatgtatt ctgacaaagc agaagtcgaa cggtgggtgc
     8821 gactgcatga aataggtgac tttcgggcag aaataacacc gctttacgca gcgccggtag
     8881 tgccggagga aatcagtacg acccacacaa tgctgcctgc tatgaatgat cagttgatgg
     8941 cgttcggtta tgtgaagggg tggaacgctt gccgcgctgc catgcttaaa ggaggtgcgt
     9001 aatgcgtgtc gcgtttatcg gtttattacc atacccgact cgtttttggg cttctgcgct
     9061 aattgcaaag ccgcatgtcc tgatggctga caacaccatc ccagcaccga aacgtcgaac
     9121 aggtattgca gcggcgagac gcgcagcaaa gaaacgcagg agagcaaaat gatgaaacgc
     9181 tggatgccgt tgttatctgt gatagtggca caggttttct tcggggtaat ggtgatgcgt
     9241 gtaccggagt tgtccatgct gtggcaatgg tcagttatcg gtgcgttcgc aatggcgtgt
     9301 gtcgcgagct ttattgtagg gagaatcaca ggacaggaac gtgagcaggt gcggtctgcc
     9361 ttgttgtgtt tttatgctgc ataccagacc tggttatgta gtggtgcgcc aaacaggtct
     9421 ccgttttcac gacggcatgg gctttgtgta aatctgtggg attactgtga ggatgcaggg
     9481 tttcccatgt gggtcattcg agcggcgtgc gtgcaattgc acaaagattt tgcacgagca
     9541 gggcggaatg cacagcttcc gtttaacgca gataacatgt cgtattctgc tgagtcgtat
     9601 cagcaggtgt gtcatgaaaa tcctgcgcgt attgcgtggg tcaatgacca gttgcagcag
     9661 ttaacggagt aaaggtgatc acaatgaccg acaaccaaaa agttttctgt gtattggtaa
     9721 tgattgtcgt gacggccata ctcgttggca tcttcgcggt tgaaggtccg gttcctgcat
     9781 gggcagtcgt ggttaatcat cttatgtcag ctgcgacgtg ggcgtatgta ggttgtgttt
     9841 ttatagggaa gaaattttat ggctgaatac aaaagtccgg ttgtgcacaa gctgaaaatc
     9901 ctgccgaagt atttttctgc tgtgtgtgca ggtacaaaac gtgcggagtt acgtaagaat
     9961 gacagagatt accgtgcggg agatgtgctg cacctggttg aagcatatcc gggtggtgat
    10021 agcggcatac ttaccgggca gcagttgtct gtaagcgtta cgcatgtagc agatgtcagt
    10081 gcgtatgcac cggggcatgt gctgctaagc attgcgcctg tgtggatacc ctgtagtgag
    10141 cgaatgccgc cagttggtgt tgatgtacaa ctgtttgtgt ccagcacggg tgaacagttt
    10201 acaggtttta acctggataa tacgggtgat ttccagtatg cggattgttt cggcgcgccc
    10261 atcgtgtgtg aagccacaca ctggatgccg ctaccggaac cgccgataaa acagggggag
    10321 taatattatg gggaagaagt acgagttagt gaaaggccag tctgtggtcg ttgaaggtgt
    10381 cacgctttac cggattcgtg cgttacgtga tattccctgg cgctgcgtta atgcggggga
    10441 tttgggtgga tatgtcgaaa gtgagcgtaa ccttagccag ggcggtaatg cgtgggtgta
    10501 tggtgacgcg aaggtttcag gcgacgcgcg ggtatttggt gatgcgcagg tgcacgacaa
    10561 ggctgttata tccggtcatg cgcgggtatc tggtcgcgcg cagatatcag gtgaagcctg
    10621 tattcgtggc atggctcaag tgtgtggtta tgctttagtc agagatcggg ctacggtctg
    10681 tggaaatgcg attgtcatgg atgaggcgct tgttgagggt gtcgctgaaa tctgtgacca
    10741 tgcggtggtt tgtgacgatg cgactgtgaa agatgcgaac atacgtgacc aggtgagcgt
    10801 cgcaggtgat gcttgtgtga acggacatgt tgtcatcatg ggtatcgcat tattgtatga
    10861 ctgtgcctgt gtacggagct gcgaggactg gcttactgtg agtgatttcg caggcactgt
    10921 ctgtttcata gatgattatg gccagaacat agaatgtctg cacttggatg ggccactgac
    10981 agcatatcgc agcggtgagg gtgacattat agtacgcgcc gagggctaca tggatacgct
    11041 tcatgagttt gacgagtgcg gggatgctac atgtaaagca cttgctgcat ttatccgtgc
    11101 acaaatgaat ggtgaaacag ggggaacagg tgagtgagaa atatgagtat ggcagcacct
    11161 gttcggggaa gccccgattg acgcattata caatgcgtgt atagtggtaa tgttgggaga
    11221 caacgatggt cagtaataag gcagtgcgta atgtgatggc actgaccggg cttattctga
    11281 tgttcctggg tatcgtactg gcagcattag gtgtgctgct tactgcgtat cactggaccg
    11341 tcggtaatcc ggtaatgccg tggctgtatg tgacaggtgt tgcgctttgc gcgctacttg
    11401 ccgggtcgtt cctgatggag tacggcggta ttaaaccaat gccagataag cggtagcgtg
    11461 agttgtctgt tcatgctgcc gcatatataa aggggataaa gcatgacaga cagtgaacgc
    11521 attgcacaac tggagcggga atgtgccgca ctccagaaag tgctggagcg gtttgttgag
    11581 ttcgcaggta aagtgcagcc gatgtataag gagtgggagg gcggcacggg gaagttcgcc
    11641 ggggtaagtg cggcagaaca accctccgca ccatctcccg gtcaacgtaa gcacatcact
    11701 tacgctatgg cgcatgaggt gcgcagagag cgtgccgaac atccggaaga gagtgtacgg
    11761 gcactggccc ggcggctgaa catccctgaa agtactgtgc gctcgtgtct taaactggat
    11821 gaccgacgta tgcgggagct taaaaagaaa gatatgcgca gtgcggatga agcatatcgt
    11881 ctgacagggg gggatgcctg atgtcatata tgaaaatatg gcgggattta tggacgactt
    11941 cggaccttgc gcagaagcgt attgaaaacc ttagtgctgt gattacacaa ataaacacac
    12001 agtttattcc gaagatccac gaccgcctcg ccgcatctga gcgtcacgtc aagaagtttg
    12061 atccgatgct tgaacgctgg gagaacggat acctggtccg cacgcgtgtc gtgcgacaac
    12121 gtgtggaagt tccggttgcg gtagatagtg gtgagcatga tgatgtgtca tcacagtggc
    12181 gtcgtgtgcg tcgaatagac tatgccacgt atcaggaagt tatgcggctg acagaggaag
    12241 gcttaggtat tcatcggatt gggaagcgat taggcattag tccacagacg gtgagtaaca
    12301 cacgaaagat gccgccagaa cgtgtcgaga aattacgaca gttatatcag ggtgattctg
    12361 gcgttgatgg tggtgaaagg aaagatgagg ttaatgaatc taaaccacaa agaaagccac
    12421 agaaaatagt tgattatgag atatatcaaa aagtaatgga tatgctgaac agtggtttga
    12481 gtgcatatcg tattagtaag gagttaggtt ttagccagca aacgataagc aatattaggc
    12541 atatgtcgcc agaacgtgtc gaacggttac ggcgactgta tgaaaatggg gagcaggtca
    12601 gaccgtgtta tatacctacg ccgtttgatg gtgatacatt aaaaaaaatc attagtatac
    12661 aaaaggattc tccgaatatt ccgttatcgc gtttaagcgc attagtaggt gtatctgtgt
    12721 atcatcttaa gaagtatcta gcgctctctg acgcggaacg tgacgcatta ctggaggcgt
    12781 tagatgaaga cgagtgagtt gtgcgacgca tatgatcgcc ttcaacaacg cgttgaaaac
    12841 cttagtgctg tgattacaca aataaacaca cagtttattc cgaagatcca cgaccgcctt
    12901 gccgcatctg agcgtcacgt caagaagttt gatccgatgc ttgaacgctg ggagaacgga
    12961 tacctggtcc gcacgcgtgt cgtgcgacaa cgtgtggaag ttccggttgg tgactctgag
    13021 gctgctaaga cagaacatgc tattagaggg aggcgcactc gtaaggaagc tgattatggc
    13081 acatatatga aagtggtaca gatgttgaat gacggtttga ccgcttatag gataggaaag
    13141 gaattaggta taagtcatca aatggtggca aattatagaa acatgtcgcc tgaacgtgtt
    13201 gaacatctgc gtctggttag tgagggtaag gttgcattat atgataatcg tgtgcgaggg
    13261 atggcacgac cgctcacaaa tgatgaagtt caaaaagtat tagatataca agcgactatg
    13321 cctgatatcc cattatcacg tttaagtgtg ttaactggtg tatctgtata ccgtcttaaa
    13381 aaatatatgg cgctctctga cgcggaacgc aaagcgttat tgagtgctgt tactgacact
    13441 acgcccgtcg ttaatactgc gtcagatgtg actgccacac agtcacgaac gcggaagtca
    13501 cggaaaggtg acgatctgaa tgacccggta tttttctctg atgactctga tatgtaaggt
    13561 agctctatga caaacgaaga agcactggaa aaggcacgcg ccagtgtgct ggcggtgaat
    13621 gagtcacttg atatgctgga gcagaaaacg gcggaactgg tacagcaatg tgaaggcata
    13681 gtgacacgta tgaatgctgt ggagcagaag ctgaaacaga tagaaaaaat gcacgcggat
    13741 gtgcagcgga tcatgtcggg caagaagtga cgtatgcgta gtgcgcatat gataactgtg
    13801 aaaggggaaa agacgaatgc gtaaaattta cgaggcggag attaagcgcg cgaaagcggc
    13861 tgaggcccgt ggggaaaaac atacccctgc ggtgcagaag gtgtcgcgct acgcggtaga
    13921 tccacggact atccgtatca ttgaaggctt taatatccgc aagctgtcgc aggagcatat
    13981 cgactccatt gcgaagtcat atgcggcagg gcagccagtg ccacctgtgc tggtcgctgt
    14041 tgcagcggat ggtgttcttg aactggttga cggggaacat cgtgttcgtg cggcaattca
    14101 ggcagacatg cagcgcattg atatccaggt gttttatggc actgaggaag agcgcgtggc
    14161 agtggctgta tcgtccagcc aggggcgtaa cctgacgcct gatgaacgtg cgctggcata
    14221 taaacgactg agtgataagg gctggtccaa cgatgagatt gcggagaagg tcggtcgttc
    14281 cgctggcgat gtggctaacc atttgttact ggcacagtgc ggtccgaaag tcctggagta
    14341 cgttgcatcg ggagaaatta aagcgacgcc gttgtttagt ctggcgcgtg agattggtcc
    14401 ggcggcggta cttgaccggg tggagcaggc gctggcgcga aaacgtgccg cagccacacc
    14461 accaccggaa gccccggtgg aagagaaaaa gaaatcaggt cgggggacga agccggggtc
    14521 cacgaaagca ccgcgtccgc gcattcgtgc atctgacctg acgctgtttg gtaagaaaga
    14581 ttatgaacgt gtaacggaga tagtggccgg gttacggttt actagtgaga ttaccagcga
    14641 tgacgcggat gtaatgctgc tttgtcgggg tgagcttgcg cgggagttga aggaactgct
    14701 taaccgttat gccgtggcaa aaggtgagga gtagtgtatg acagcacatg ttatggtgga
    14761 catggaaaca gccggaactg gcccagatgc ggcgatttta tctgcggcac tggttcagtt
    14821 tatccctgag accggggaga ttgtgtcatc gtttgcggtt gatattgacc tgaactctgc
    14881 ggtaaagcat ggtggggtaa ttgagccggg tacgatgcag tggtgggcaa aacagagtga
    14941 caccgcacgt gaagtattta attcgacgga cgctgtgccg ttccccttgg cgatgtacag
    15001 tattcaggaa tggctggatg cgactggccc ggcatatgtc tggtctaatg gtgccgggtt
    15061 cgactgtgtg atactccgtc gtgcccttga acgtctgggc tataaagcat ggccgttttg
    15121 tatggattgt gacgttcgta cgatggtgat gctgggccag cagactggtt ttgacccgaa
    15181 aaaagatatg ccgtttgaag gtacggaaca taatgcactg gatgacgcac ttcaccaggt
    15241 gaaatacgtg tccgctatct ggcagcgact tgtcgggggt gcatgatgac acgcctggtt
    15301 tgtctggatt tcgagacatt ttatgacaag gactactccc tgagtaagat gaccacggcg
    15361 gcgtatatcc gtagtccatt gtttcaggtg atatgtatga cggctacggt ggatgatggg
    15421 cctgttctgg aagcgtgggg atacgatgac gtcaggcagt tgttattgtc cctggaactg
    15481 gacagaccgg acacgaccac agtggcacac aatgcgcggt tcgatggggc tattatcgag
    15541 tggattttcg gcatccgtat taaccgtctg ttgtgcacca ttctgatgat gcggcaggtg
    15601 gggctgtccc gcctgatacg tgaaagcctt gcgtcagtgt ctgactttct gcgtcagcgg
    15661 gggtatccaa ttccggcgaa ggggcatgag gttgagcgtg cgatgggcca gcggcttgag
    15721 cagatgacgc cggagttccg ggcggcatat ggtgcttact gccgcaatga cacagttatc
    15781 cttcgtgcgg tggcacaact gttaatgccg gagtgtcctg acgatgcatt acgtgcgatg
    15841 gatatgacac tgacgatgta cacacgtccg gtgtttgtgc ttgataaacc gttgctggag
    15901 aagtatctgg tcacgttgcg tgagaaacgc caggctgcat tacaggagat ggcgcgccag
    15961 tatggcttcc tcaccacaga tgacctgctt aactcactgc ggtcgaagcc gaagctggca
    16021 gagctgctta aggggctggg tgtggcgcct ccgatgaagc tgtcggagaa gaagacggag
    16081 acggcgaagg cgaaagcact ggtgcaactg gacattctgc gtaacccgga agcatctgag
    16141 aaggagcaga agaaggcgtg gaaaactctg gcaggtcttg ggatacagag cgacacaccg
    16201 gatggtgttg ctgccgagat accggaactg gaggtttatg actacgcttt tgcaaagact
    16261 gacctggagt ttaagcaact ggcagaccat cctgatccac gtgtggcggc gcttgttacg
    16321 gcgcgtatgg agaacaacac gtcaatagct gagagtcgta cgcgctcgtt actgaatgtg
    16381 cagaatccga tgcctgtgcc gttacagtat gcgaaggcgc ataccagtcg ctatacggca
    16441 gatgaaggga ttaacgtgca gaacatgccg aagcgtcagg gtgacaaaac actgcggcat
    16501 agtatcactg cgccggaagg catggaaata ggcgcagcgg actccagcca ggtggaggcg
    16561 cgactactgg cttatgccgc acaggagcac agcctgttgt ccatcttcca gactaagggc
    16621 gacccgtaca gccatatggc cgcagatatt taccgtgtcc cggaagatga tattaagtac
    16681 tggacgaagg gggagggtgc ggcagagcat gaagcacatt tcagtgaccc gtcacatccg
    16741 agtcatgccc aggctaagcg tcatacgttc atgcgtaacg taggaaagga gactatcttg
    16801 ggttcgggat accagatgtc gggggcgaag ttcggtctgc gtctgcggca gcagggggtg
    16861 cgtttgcgcc cggagccgga gcagatgatg gagtggattc ggaaaatgca ggcgtccggc
    16921 aagtggccgt cagagccggg ggagcaggat gtgttaatgc gggagtattt gcaggcgttc
    16981 cacgatgcgg aggcccgacg cattaactgg ctgtatcgca gtaagcatca acgcattaaa
    17041 ggtttttggg agacctgcga gtgggtgctg tcgcgtatgg ttgccgggga gcaggggcat
    17101 ttcggtgggc cggatggcat gttgttctac tttgacgggc agcataaagt gttcggtgaa
    17161 catgcgccgg gtgttatgct gccagatggt tactggatag tttatccggg gcttcggtcg
    17221 tttgttgaag aggaaacagg ttttactaag tatacctgga agcggatgaa ggacgggcgc
    17281 atggtggacg actttatcta tggcgggtct atgacggaaa acctgattca gggactggcg
    17341 ttcgcggcgc ttaagtggca ggccgtcaaa gtgcaccagg ttgttccagt gaaaatgaac
    17401 gtgcacgatg aatgggccag cgtctacccg gtggcgctgc gtgatggtgt taagcgggtt
    17461 tacgagatgg cgatgcgagc cgcgccgccg tggttgaaag gtatgccgtt tgactgtgaa
    17521 tttaaatgcg gccccgacta cggacgctgc taaggagcgc gtaatattat gggagtgaaa
    17581 ccactaccga taacgccgtc gttactgaat acatttttaa cgtgtccacg gcagtatgag
    17641 gcgaagtaca tcacgaagga agtggtgttt cagcagacgg aagctgcggc atatggtgac
    17701 aggatacaca aggcggtaga gagtgcgtta aagcatggtg cagcactgac accggaagct
    17761 gattttatgc agccgttggt agactggtgc cgcagtatgg cggcgcgacc gggcactgag
    17821 atgtttgtcg aagaaaagct ggcggttact catgcactgg ccccgtgtag ctggcgtggt
    17881 gataaaggta tggccccgtg gcagcgtggt atcgcggatg tgttttttat tgaccatact
    17941 aataagctga acattgtggt ggactggaaa accgggaagc tgaaagatga cagaacacag
    18001 cagcacatac tgtcactgtg tgcatcccgc aagacagggt attcaaagac actgtgtatg
    18061 tgggtgttcg tgaaggagga agacctcatt acgcatgtac ttaatctgga agggctggac
    18121 cctatcgctg tacatctcca gaacgtgaag gcgtatgagg aagcctgtgc aaacaatcac
    18181 tttccggcga tacgcaatgg tttatgcggc aagtggtgtg acgtgcttag ctgcgtacat
    18241 aacgggaaga acccggcatt taatgcgaag tgaggggata catgacacgt tacgaggacg
    18301 acttctatac ctggacgcag gagcagcgag agttactcgc ggctggacgg tttgatgaac
    18361 tggatattga acacctggcg gaggaacttg acagcatggg ggccagcgag cgtaacgcac
    18421 ttgagagtcg gttaattgta ttgctgatgc acatgctcaa gtggcagtat cagcctggtc
    18481 gtcgggggcg tagctggcag tttacgatag cagagcagcg tcggagtatc acgcgtcatc
    18541 tgcggaacaa tccggggctt aaaggccgtc tgtcggagat attcacagca gcatacggtg
    18601 acgcacggta ccgtgccgaa tacgaaacag gccttgcgat tgacacgttt ccggtggatt
    18661 gcccgtggac gtgggagcag gttattgacc aggagtttat gccgcaatga cacgttacga
    18721 ggatgacttc tacggctgga tacaggagca gcgagcgctg cttgctaccg gacggctgtc
    18781 cgagcttgat acggttaatc tgatggagga acttgcggca gtggggcgta gtgaatatcg
    18841 tgaactggtc agtcgtctgg cggtactgat ggcgcatctg ttgaagtggc agtatcagcc
    18901 ggaacgtcag gggcgaagct ggtctttgac catagcagaa cagcgggatg cgcttactga
    18961 tgtactggat gatagcccga gtttgcgtgc acgactgccg gaagcgattc agagagcata
    19021 caagacagcg aaaatatctg cggagcgtga gacggggata ccggggcggc agtttcccgt
    19081 ggattgcccg tggacgtggg agcagttaat ggatgaggga tacctgccgc attaatgcgg
    19141 agtgacacgg atgtttgtgt tcacaatgag ggagcataac attatggcta agacaccaga
    19201 ggggtgggtt aaggaccgga ttaaagaaat cctggaagaa cggaagctgc attacttcat
    19261 gccaatgacg atgggttacg gtaagtctgg cgaaccggat ttcgacttct gtctttatgg
    19321 tatctatgtg aagctggaag cgaaagagga cgcacttcgg caccgacggg gtgagcgcat
    19381 tacgcggggg ccgaacaaag gtcaggtagt acggcagggg gcaccgacga tattgcagca
    19441 acagacgatg tgcgatatcc gtgccagtgg cgggattacg ttactgattg accgccataa
    19501 ctacaaaggg ctggctgtgt tcattgacga agtgcgtgct ctggtgcagt ctggcacgct
    19561 taatctgccg tttaccggat ggatgcggca ggtgataacg tgcgcaatgc gcaatgatgt
    19621 ttattatggt catgtaccag aggtcatatg tccggttata caatgattaa gcctggtgcg
    19681 tggctgatgg aggataagac ggtgctcgcg cttaaggtcg gcgacgccgg gcgtatcgct
    19741 aatgcactcc cgccggggac agcacatgtg acggcgcatc atggggcgca gcttgttgcg
    19801 gtgccatacc atatgcatac gatgcgtctt ctgcggaacc tgggctatga cacgggaggg
    19861 atggaggtat tgcggcacac gtacccgctg ccgaaggtgg aagggattta cccgctggct
    19921 aagcaccaga tttcacaggc ggcatttttt acggagaatc cccgcgcttt ctgtaccagt
    19981 accatgcgta cgggtaagac cgcatcggct atcgtcggtg cgcgtttcct ccaggagaag
    20041 gggatggcga cgggtgtgct ggttattgcc acagtcagta accttgtcgg tgtgtggcag
    20101 aaggagatta acggtctgta tcctcatgcc acggtgaacg tgttgtatgg tgacaaggcg
    20161 aaacgacggg aattactgga gcaggacgca gacttttaca tcattaacta tgacggtgtg
    20221 aagttactgc gtgatgaact gggcgacgcg gtgacgagcg gccgcatctc tgtctgtatt
    20281 gtggatgaac tgacgcacta tgcgaatacc cgcacgcaac tgtgggaggc agcacattgg
    20341 gttattaatg gcgcacgctg ggaggtgata ccgggcaaaa ccatgcagga tggcgagcgt
    20401 tcctggaaga cgaaggaccg gaaaaagtat ctgccgtcgg ttaagggcgt tgactattgc
    20461 tggggtctga cggggacacc gggtggtcct gagatggtct atggcgagat taagctgata
    20521 cgtcctgagc ggataccgtt ctcgtttacc gcatggcgtg acacggtgtg catcaagcag
    20581 ggcttcaagt ggataccccg agaagggtac aaaaagatta tccatatgca gatgcagccg
    20641 tgcatccggt tcgacaagaa ggacatcatg gacctgccgc ctgtggtgat gtctggtcgt
    20701 gactgtgact tgtctgccag tcagaagaaa gtttatcgcc agttgcagcg ggacatgctg
    20761 gcgatggtgg gtgacgcgac gattaaggct gttacgaaat ccacgctggt atcgaagttg
    20821 ttacagacgg ccgcaggtgt ggttatcacg gataagggca cagaggcgct ggacatgcag
    20881 ccgcgtctgg atgcgctgga agagattatt aaagaggcca cacagaaggt cgttgtgttc
    20941 tgtgcgttta cggcagtgat agaccgactg aatgatgaac tgacggcgcg gggttacagc
    21001 gtcggtgtgg tggatggtcg tgttactggt gcgaagcgtg atgcgatatt ccgcacgttc
    21061 caggaagatc cgaatacata ccaggtcatc ctgtgtcatc cacgaacgac ggcgtttggt
    21121 gtggagctgg ctgccgcaga tacgatggtg ttctttggtc ctccgttgtc tggcgacttc
    21181 gtctatcagc aggcgattga acgtatgagt tcgctcaagc agaaggcggc atcctgtcaa
    21241 attattcacc tgtcatccac atgggaggag cgcaatttat tcaccagtat ccataacggg
    21301 gtcagtatta atgaggcaat caatgagatg tttacccgca aagaggatta gacactcgtt
    21361 gtatagttga atattatgta agtgggataa caacatggaa ctatcagaaa tggttacgaa
    21421 tctgcgtaag attcgtgagg ccagacgcga atgtaatcgc gttctgaaag aaattgatga
    21481 acgtgaacag gcgattaccg ggtctattct caccacgatg cgagccagcg gccttaagac
    21541 ggcccgtttc gacggtatcg gtacggtgac agtatctacc cgcgaccacg cagaaatccg
    21601 tgacttcaat gttctggccg catttatggc gcaacaatgc attaacgctt ataaccaggg
    21661 gcttccggtt accgatgcct tcggcttatt gcagcgtcgc gccaacctga gtacggcaaa
    21721 agaattgctg gatgcaggat acaccgcaga gacgatggga cttgccattg tcgaaaagcc
    21781 cagcctttcg ttttcagtta aataaggaga atggtcatgt ccactaattt acccgcaatg
    21841 ccagcatata tcgcgaacat gatgccgaca ttgccgcagg ctatgcgtga acagcttgcg
    21901 gcagcatttg ctccggcact ggattccttt gggtcagcgt ttaaccgaat cagtctgaaa
    21961 ggtagccgct tccaccttat ttccgggggt caggtcactc acacatttaa tgaggcgttc
    22021 acgtttgtca ttgtggcaat cagcccggaa caacattgtg tctggtataa gaacggttat
    22081 aacggtgaag agggggtaga gcctgatgct gtatgggcac agaatgaaga gccacctgcg
    22141 aacgtaccga tgtctgcccg tgtgaaagat gcgagtggtc gtaaccagta ctccatgcgc
    22201 cgtcgtttca ttgtcgcgcc gtatatggtt aatccccagg gtaatccgta tgtggacctg
    22261 gataacctgt atgtgtggga cgctggcggg atgactacgt ttggtgatga tgtccagttg
    22321 ggtagcggtc cttaccctaa agcgcactcg ttcagcacgt acatgaatat gtgtaagtct
    22381 tccgggtttt atccgtgtgc cattcttacc acggcgattt ttgacctgcg gcagtccgtt
    22441 ccggtggcac gtttcctccc tgtttgcagc cagcagggta cacctgcaat tctggatatg
    22501 gatacgctgc aacgtgttat tgaaaaatcg gtagatccga ccacacagca actgcttgac
    22561 tggcgtcgtg gccgcagtgc ggaacaacag cagcaggatg taggtacgcc tgtagcacaa
    22621 caaccagtgc agcagacata tcagcagaca tatcagcaga catatcagca accagtacag
    22681 cagtctgtac aacaacaggc accacaacag tcatatcagc cagcaccgca acagcagacg
    22741 ttccagcacg cggcgcagca gcagcaggta cagcagcagt catatcagcc agcgccacag
    22801 caaccagttt tacaagagaa cagcactccg gtatatcagg agccgcaatc aggtacgcaa
    22861 tcctttatgg atgagtttgc taactccccg actcttgatg gtgttgtaac acagacaact
    22921 tctccggaag cgggtcatgt ggatacagtg gcatctgaca gtacgctcgc aggtgtggtt
    22981 aaaaagatga gttccgcagt tgatgaagct aatcagacta agcaggaact tgttcaggaa
    23041 gtagtgcagt cacagccgca gcaacagcag ttaccggaac aaacagatgc taccgcagaa
    23101 gacctggttg ctcgcatgaa cgatattatg aacacgtttt aaggagtgag ctatgggacg
    23161 tccgggaaca cagatatccg cactattaca ggccgcagac cgtggcggta ttccggtcgc
    23221 aaaactggcg gcactgctgg gtgtcagtcg ggggatgatg tatttctata tgaaaggcag
    23281 taatcccagc ccggatgtcg ctattcgcgt tgtcgagctt acccgacgta ttcatgatgc
    23341 actgcgggag ggcagcctcc cgcttcctga acgtgatttc gatgcggaat taaaagccgt
    23401 tttgaatatc actcaacact gaaaggccac gtatgagcac tgttttagat ttccttcata
    23461 gtgtgctccc tgcgtctggt aacttcttct gtgcagcggc tatcgaacca aactccggga
    23521 aaacgaagct atatccagta acgtcgcatg aagaactggc acgtctggtg cagaaaattg
    23581 cgaataacag actggatgcg tacttcgcgc tggcggcgtt tgaacaaacc tggcatgacg
    23641 acccgaaagg acgtcctggt aagaacggtg cagtgaagca agttttccgt atccaggaga
    23701 atgcgtatgc catgcgtgca ttatggctgg accttgatgt cgatccggat gacacgaaaa
    23761 agtacccatc acaaggtgcc gctattaacg gtattacaga gttctgcaac cgtaccggga
    23821 tgccgaaacc ttgtctggtt agctcaggtg ggggtatcca tgtgtactgg tgcttcacaa
    23881 acgacatatc tgccgctcaa tggaatgtgt tgtcatcggc gcttgacgca gtgcttcgcc
    23941 atatgggtgt accagttgac tcgacacgga ctatggatgg cgccagtgtc ctgcgtcctg
    24001 tcggtacaat taactggaag gagaagtacg ggccgggagg cgcaccagtc gcgctcattt
    24061 ctcatggcga catagtgcat gtcgatgtgg tggttaatgc actgtcacgg tacatggaca
    24121 cgcacgggat tgtccgaacc cggaaaagcc ctgcgcgaca aacaggcact gccgcacata
    24181 attcactcca gcatctgatg aataacccgg cgttcatggc ggcaatgggg cacgtcatgc
    24241 aggaggcgaa ggataaggac ccggtcctta ttacgcgtga gtgtcgccag attcgcgaag
    24301 ccgggcttga ggcagaaccc acatggcatc gcatgatgtc ggtaatggtc tgttgttttg
    24361 gcggcgaaat cgcagcgcgg ataatatcgg catacgataa acggcgtttt aatgagcagc
    24421 aattcattaa caaattccgt tatgtggaaa gctcctcccg tggccccgcg tcctgtcagg
    24481 agtttaaccg cctgtgtccg ggcaaatgtg atacatgccc tcacttcggg aaaatcacgt
    24541 cacctgctga gcttggacgc acgcagcgta atattatgcc gcctgctaca cagacacaga
    24601 cacagacaca gacacagaca cagacacaga cacaggtagc gtctgttact actgccgcgc
    24661 tgggtaagcc tgttactaca ccgacgcctg tttcgccaca ggggccgata aatcttaggt
    24721 caataggttt tgagtatatt cagggtgagg gtttgtacta tcttaaacct gaaaaagacg
    24781 aagcaggaaa tacgacatat tttaaacaat ttattatgtc aggttatatg tttccggtag
    24841 ggaagcagga gatacatatt agttttgcac agatgcaaac tatatatatc tggaagatta
    24901 tttcgataac tggagaagta cgacaggcgc aaatgttagc agaagaccag gccacgccgt
    24961 cggcgctatc taagtggtgt gcaaataatc agttaatagc gttcccaggc gcggacgaag
    25021 tcaggaaccg ttatatgaga tcatatctgc ataaggttat tgattccatt cccagcgttc
    25081 gtgtggctga tcagttcgga tggacggagg catcgctggg ccgcaatggc agtgtacgtg
    25141 gttttattct ggggaatacc atgttaatgc ccaatatgtt gcctacacag gtggctatta
    25201 atgccagtct ggagcagttt ggccgttccc gttataccgc taccgggtca ctggaactct
    25261 ggaaacagat acccgcagca tgttcccggt ataactggtt gccaatgcag gttgcgttat
    25321 ccctgggatt tgcgtcagta cttatggagt ttgctgtggg gtcaacgcag aacatcatag
    25381 tgaatttctg tggcccgtca gggtccggga aaactactgc gatgcgtgcg gcacttagca
    25441 tatggggaaa tcctgagcgg cagatgactc gtccacagga cactaataac agtcaggcgc
    25501 gaatagccgg actgtcacga agtgttccta acggccttga cgacgtaaca aaacttgagc
    25561 cgaaagattt gtcggacacg gttttttcgc tgtcagaagg gcgagagaaa catcgcctgg
    25621 agaagaacag tgcactgttg cagggacagg aatggaagaa agttacgatt atgacgtcga
    25681 acacgtccgt acttgatgcc ctggtgccgt tcgccccgga ccgcgatgca gaagttaagc
    25741 gtgtgcttga gctattcatg cctcgtgttc ctgacagacc tgcggaggaa atcgcagaaa
    25801 tgcagcggct tgagcgtgta atgcagcaga actacggaca ggccgggaca gcattcgttc
    25861 agtggctgat ggatacgcca ggtatgattg acagacttcc gggtctgctg gacaagatgc
    25921 agatgaagtt cactgaatat caggatgagc gtttctggag tgccacgctg gctgctgttg
    25981 ctgtggttgt gccgatgaca aatgcggcag ggcttactgc atttgatgtg aagcagatta
    26041 taagtttcat gctggagcac cggaacagta tgcgtaagca gttgctgaac tcgaaagtcg
    26101 acggcggcag tttgttgtcg gatttcctgt cagacagtct gcgtaacacg ctcattgtta
    26161 cggataaaga ccgtccggtt actgttaatg gagacatgcc gctacaccag gatgcgtatg
    26221 tgttacgtgt gccgtcgatg gcggggaatc tggatgttcg catagagcgt gaccaacagg
    26281 ttgcatatgt ccgttgtgcg gcattccata agtggtgtaa ggaccgcaat accacaccac
    26341 gccatgtctt gtcggaactc gctgtaagcg gcgtatatga tgcacgcgtt aatggaggca
    26401 aagggtacat taatatgcgg ctgggtcgca gcatttccgc actggcgcag atagcgccgt
    26461 ctcgtgtgta tacgtttaag ctggataagg agggtattga cgtggatgtg cttgcgagca
    26521 agacagcgaa gtcagaggct gatgacgatg aagtacatta tcaggctgaa caaggttaaa
    26581 gaccgtaaag gtgaggtggt aagcgtgccg cctcacgcta tttgtttcct gtatggattg
    26641 ttgccgaagg actgcgtgat ttgcggttct ccggcacatg cggcactccc ccctgatgaa
    26701 gcgcagcatt acctgccagt gccctgtatg ggcctggcaa tgcgctggga ggccatacag
    26761 gatgagttta aagcgggtat cgacaatgag gatacccgca ggttgaagga gtatttactg
    26821 gcggtgggtt agttctgtat ttctttatat taaaatagtg ttggatattt cctgtataat
    26881 tttagtttta tttctaatca ggggatgcgc tggattatgt atatgattat tttgcagata
    26941 gataatacac tattgcgtaa cacatatccc ggtaaatatt tgacacttgc gtataaagat
    27001 attcagtcgt ttatgtcacg ctgggatttc tattctgtaa aaggtactgg ctgtgtattt
    27061 gtaggcgata atctacgtgc accatataaa gatgatatat gtagcgatgt tttatccaga
    27121 ctgattaaga cgcataaatg gcttgcccca gcattatacg aatttaatat attttgggtt
    27181 gatgtggaga atgagcaacg gctttgcgta ttggagatgt gtggagaatt atcgcatata
    27241 tcctcgcatg ggaaactacg taccagttaa atatataaaa ggtgaagcct tgttaatatt
    27301 atggaacgtg taggtcgtgg tgaagaacgt gtgtattcat ctgaggaagt acgggccgac
    27361 cccggtctga gcaaccaatt gtgccgaaac ggtagcacag tgcgccattt gtggtacact
    27421 atgaactgtc atatagatgg atacagcata tgataggtag ctttaaagat ggacctggcg
    27481 gcgaactagc ccggttttat ttcacaggtg tacgtggtcc ggggattccg aatcagatag
    27541 aaaaagcact gcgacgcaaa ctgtcattgc ttgatgcagc aagttgtgaa acatcacttt
    27601 ttgttccacc gggcaataat tatgaacgtt tgcgggggaa cctggatggc tggtccagca
    27661 ttcgggttag cctgcaatgg cgactcatat ttcagtggcg tgacggttac gcatatgacg
    27721 tgtatctgga cccacataca tatcggtaag gagtcatata tggatacaac tacagcggaa
    27781 ccgaccacag taggcgagat gttacttgaa gagtttatga aaccaatggg tatcacgcag
    27841 attgaactgg ctaaagcgct gggtgtttct cgcaaagttg tcgggcaaat tattaacaac
    27901 agtcgtcggc ttagcgttga agaagcggca catctggcgg tgcttttcgg cgttgatgaa
    27961 ggtttctgga taaacttaca ggcggcacat gaccgttggg agagccgaca gatgcttgct
    28021 aaaggtcgtt ttcgtggcgg ccatgttttc tgtgccgcat agcgttttct gattagtgcc
    28081 gtagtatagt tgttgttaga agaagtgtga cttcgccggg tcagtgtcgt aacattatgg
    28141 atgttggata ctgagtgtga cttcttcaag tcagagccgt aatatagcgg ttgatttgtt
    28201 aggagagaag gaacctgttc cggtcagggc caccaacctg gtaccggaaa ctaaaagccc
    28261 ggctggttac cgggcttttt tattcaacac cgccgccagg tcgtccctgt gccataagtt
    28321 cttctgtttg ccgcatggat tcacgcaaac gagcagacgg tttacgcgtt atttcaaacg
    28381 gtaagccctc attacggacg acattttcgg caaatagccg taatgccgca ctgacggata
    28441 atccgcagcc ttgcaggata gtttcaaact ggtttttgag cgtgtcgtca agccgtgtac
    28501 ggatgtacga ttccatttct ccccctgttg ttcagatatt acagtgtctg gtgtagccca
    28561 ctatggatta cagagcaagg caggtacagg gggacgatgc cccctagaag tggtactggt
    28621 tccagacgtc acggtctagt gcaagctggg cgatgcactc gttaaggagc cgggtattct
    28681 cgtcggcacg gcgtgcctca ttcccatact gttcaagagt ctctcgtagt tgtccagaaa
    28741 gtttacgggc ttcggctctt tcaggtgtgc aggtatcgcc ggaaggggga gtttccctgt
    28801 cttcacgggc aggtagcggg gggtcgtaca cgcggtcaaa atgctgcata acacgattaa
    28861 gcaactcttc ggtgcgtgcg gtatcagact ggatatcttt agcatattta ctctcacggt
    28921 cgcgctgcgc atcaagtgat gcggtaagga cggtgatatc ctcggtcacg ttattcagcc
    28981 cttgctgcat gatgctgtta gcactgtcag taactttcag ttgctcatgc gtcacgcctg
    29041 taaagtagcc agcggtaaat gccagggcga taagcacacc gccagcgata atgttacgca
    29101 tcgggtgtct caaacaattc tcgttctttc attcgacgtg cacgcagtat cggtttaccc
    29161 gctgcatatt tccacgccat gatttgttct gccgcgccgt cgtagtcatg gcggttcagc
    29221 agtgtcagta gtgtggacgg ttgcccacct gtcagtgtga taatgccgct cttaccgcct
    29281 ttagtgccgg ggccgacatt aaataccagg gagaccagtg catcgaactg gttctgtgta
    29341 agcggcacat ggacgttatt gtttatggca cgttcggctt ctttcaggtc ttcttccagc
    29401 agcgcatcca cttcttcctg tgacagtgta acgccgtcgt agcgggtgtc ttttcgcgag
    29461 accaggtgcc ccacgccgat agtcggatta cctcgtgcat ttacggcacg ttttatcggt
    29521 cgtcctgtgg catcgtcata cagggtatag cgtatgcctt cttcggcttt gatgaattta
    29581 cgcccgtttg cggatgtttt catagtatcc ctccttcggt atcaggaaac tgacgttgcc
    29641 tttgtagaga atgacgggga tgaacagcag taactgccag atgacttctg tgcgggtcat
    29701 ggggcagata ttgccgacgt ccgtccagat gcggaacagg ttaaagacgg tggccgccat
    29761 aagcagccac gcgagagaac taatatgcag cttaaatgtt gcgccattgc gtgtgtataa
    29821 catcatgtac atcaacgcgg acatattagc ggcagaggca agcataacga tgatgtagtc
    29881 cataatatta cttccgtcgt agcagggcac tcaggtcgcc gttctggacg aatttaacgg
    29941 cgcggatacc gacgccgata attaacagtc cgccaaaaaa tgccgccagc ggtagtgaca
    30001 ccatagcgaa gtgctgacgg atttcagccg cagcagcata cccaccgatg gacgccaccg
    30061 ggaggtaaag gatgcgctcc cacagggcga tctctttcgc actcaggacg aacaacagtg
    30121 cacccgcaca ggcacacagc agtgtccaca tatccacgcc gggcagcagt gcgaccagcc
    30181 cgacgctgac gacagagctg acagcaggtg tgtttccgac agtctgtaca acgctggtag
    30241 acatagcgtt tctcctttat ttaacaataa gtttcccttg ctgtacggcg gttttgccgt
    30301 cacagttagt tgccgcaaca gacaatgtgt atttgcccgg cgctgcggga acgccactga
    30361 acgtcacata gttcgcacct ttccggacct taacccatgc aggtgcgcca gcaacggcca
    30421 gttccatagg cgtactgcca gtgaatatgg cactgccatg ccactggacg ccttttgtgg
    30481 cttccggtaa tgcgatacct gcggcagtta cagcgtcaca ggggcactca tcatcgcagc
    30541 agttgtgcgt gcatatcagg tctttcacaa cagcgggagt catctcccag cgaacgcagc
    30601 tacccttcgg gaagttcagc ggtgttgtgc catcctcgcc acggtcaaca acgaagccac
    30661 cgcatgcatg cgtaatgcgc accacttccg cgcccacgtc atcccggata gttagccagg
    30721 tatggtcatc ctcttccaga attgccgcca gtcggtcttc atcctgaacg gccagcggta
    30781 acgcccggtc tgccttatcc agacggcggg taagtgcggc agagaaaaat acaggtgttt
    30841 tcagcatgat gcatctccac atgaagcttt cgctgttacg gccacttgct caatggcaac
    30901 ggggcgatta cacaggtcaa tctgcatgga tgtgtgtacg cagacgccgt cgatttctat
    30961 atcagcctgg tatctgccgg gcggcagtga ccacagcttg tcgtcaaaat agaacaccac
    31021 gcggccctcg tcatccagtt caaacgccgg ataacgcagt gtcggcacca ggtccgggat
    31081 aacttcttcg cggaaagaac agccatccca gtatgtgtgc gtggtacccc gctccacaca
    31141 taccaccgga cggatgacaa tgtttacgcg ggtatccatc agctgccacc cgtcgcagcc
    31201 aggctgcgca atacgcacag ccagtttagg cgtatcttca cacagacgga ttatcacagg
    31261 ttaagtccct cgcttgtctc gataaagcgc tccatcagat ggttctgctt gtccagatgt
    31321 tgtgagaacg tagacctcag catatccggt gacagattca tccggttcag ccgtttggcc
    31381 ttgtcattca gtgtacgatt attcttcgac cgctggctgt cagtctcaat agccagccgg
    31441 ataaaccggg cttcgtcata agatgctccg gcggccagca tacgacgcat tgcggaagcc
    31501 gctttagcac cgcgctggtt atccgggtgt gaccgttcga cgccatactt cattaataaa
    31561 tcattcgcct tatcctgtaa acgccaataa cggcgttcag acagtttatt tccgttatca
    31621 tacagcgctg tgatacctat cgatgtccag aacccgccta actcttgacg cgtggtcatg
    31681 tgtcctttgg ttacatacaa cgagtctttc tcaagccagt taaccagacc actcagtgcc
    31741 cccgccccat aggcgttaat gatgtgccgg atagactcag ggtacatgtc aataccgaaa
    31801 gtactgcgca aagctgctgc ggcatctttc cagactccgg cggtcatggt tttaccactg
    31861 ttactggcga agcgctcgcc ctcacggtaa ttaccgatat ggataggtgt accgaaatga
    31921 ctggcgtttg tcgccacttc cacgaacggt cgtaccagtt ccggcataac agcatgaaac
    31981 atcgccagcg ccggattatt tgacgcatgt tcgccggaat aactgcctgc cagcggcatt
    32041 aactcttctg ccagtgcggc cgttgcgcca gagaaatctg cgataaagtc tccaccttca
    32101 cgtactgaac gggccagcat agcacttgtc acccaggcca cacgtggcag accaaatgcc
    32161 acggggattt tgaagaaact gccgtccttg ttaaataccg gaatggcacg cgacagttcc
    32221 gtgatggtca tcttatccat ctcatcatcc ggcgcaagtg cggccagcat cgtatacagt
    32281 gcaataccaa cagcgatacg tccggcagtg actgccatgc cacgcttcgt gctcagtgac
    32341 ctgatcagat tgcggctacc ctccattgcc gggacaatga acggctggaa agagcgcaac
    32401 cattgtgttg ccgttccgtg gttatgaata ttaaacgagt ccagaacgaa catagcggca
    32461 cgttccgggc ttacgccttt gcttttcagt accataaacg cagcaaccgc aggtgctgca
    32521 ccgaacatct cgttccagtg gccaacaaca ttatccatct ctttggcata cttgcgcaca
    32581 cccttcattt tccgcaggtc tttgcggata tcctgctctg tctgcttgag gatattactg
    32641 aatgtagaca gaccgcccag ggcttccagt tccctgtagg ctttcacata ctcattatcc
    32701 gtgttccctc cggtcatgag atggttcgac aacgcagcca caatcgccgg gtttgccgca
    32761 gcaaggcgaa tactcacttg caggtcagtt gcaccgatta cctcgccatc ctcgttcgtg
    32821 atgtaacgac tggcgatatt cgctgcacgt tcagcaccct ccctcatcca gttaaccggg
    32881 gcgaacgtag gacggtactt ggtcaacagt cgggcatacg cggacgttcc ggtggccagc
    32941 acggcaagta tcgggttcag gtctttttcc ggcgggaaca gtccttccat aatagcgtca
    33001 ccatcggggt atttgtcgta aaacacaaac ttgtacttcg tggttgtgcc gtcttccttg
    33061 tgctccgtga agtagaatcc cggtttcgaa cgcaggtctc ttgctgtacc ttcgtttaca
    33121 ggtacacgca tcagcccgtg caccatatga ttctcatcag cattcacaaa catgtcattc
    33181 atgtgctgct tgaacggctg aatggagatc gacgcagcgg cgcggttcag tgattctgtt
    33241 accgtcatta atgcgtgttc ggcatagccg tcagttgccc cgtgacgttt catttcgcct
    33301 aacgggttca gtgcggacga cccgacaaag gcattcttac catcactctc ctggtggata
    33361 tacagcggca cgtagtactt aaatctggcc catgtatcca cctgttcctg cgtcagaaca
    33421 cctgctttaa tgcgcgcctg cactgacaca ttcagcgcgt cgtacagcgg ctgcgccacc
    33481 gactccagtt cttcaatgct gaaacgtgac tcaatatccc gcatggtgac tatcgcctgt
    33541 gccgaggtcc agccccctgc cgtggctacc ttttcaacag gttcaccacg ctggtaagcg
    33601 tcgaaatcct ccagggcttt aaccgccttg ttcacttctg ccgccggacg gtcctccgct
    33661 atcgcctcat ctatctctgc ctccatctgg cgacgcagtt ccgcattagc ctccgggatg
    33721 tgccgcatgg tcacccaggt catggtctcc cgcgttgcat catccacaga catccctttc
    33781 tgttccgcga tttcagcgac acgcgccagt gacggctcaa ccagtttcgt ccagtctgcc
    33841 agtatggcat cgcgtatacc cggtgcacgt ttcagcgcac tggcaagttt attgtcagta
    33901 agctcaacac cgacaggaac aaccacgttg tcatgtatcc accgtaagaa acgatactgt
    33961 gtgtcgtaca gcttcgtaac gacaggtatc atccagcgct gacggttaaa ccacagtgac
    34021 tgcacacgct ccttcggcgt ctcctgttta ccccacgcgg tactggccca tgagtccggt
    34081 atgtcattac gacggttacc accccctgcg gcaacggggg gcgtttcctg attgccggta
    34141 attgtttctc gggcttccac atcgttggcc gactcactca gtcgggcatt gcgccgacgc
    34201 cgcgcattac gcatgatgcc ttccgttgca gcaggtatcg cggcaaatgc gcccaggtcg
    34261 gctgcatcat tactcttcat ggcagacagg ctttccgggt ctgtcgttgc cagtaacacg
    34321 ttcaacgttg cgcctgtatc cagtttaccc gtagtcagtt cttcagcagc gagattgtca
    34381 gtggacgaac ggaaggccat agtgtcggtg ctgtccagac tggctggcag cttcgcaccc
    34441 ggcatcaggt cattaactgc aacgttctcc tgaatatcgc ggaacacttc gcccagcggc
    34501 ttctgctcag ggatgacata cagtttcccc tggaagccgt ttttcaggta cttgtcgtac
    34561 atgtctgccg ggacgttaat gcagcccagg ctgatacggt tatccgcagc cgtcgccgac
    34621 ttaagccgtg cctcgcgcat ctgcttcgga ttcgcggtca gtacgcgatg aatggcccat
    34681 atactaccat cggtatgttc agcaaactgc tgcacatctc cacccagcga tgctcccaca
    34741 tcatgccgtt gttgcagcat gaaaatccct gctggcgtct ggcccggaac agccgcatca
    34801 cctttgcggg aaccgtacaa tgcaggcatc gtcgccagca cgttaccacg gctgtccatg
    34861 acatacagtt ccccggcgac cttatcagca atgatatact tctggccctg attatccttc
    34921 gtctcctgca cccaggcacg caccacactg gcggtactac taagcccctg cacaggctgt
    34981 acctgcgtca ccacacccgt cgtcgcggca tgtacatcag cgacaggctg cgcgttccac
    35041 aatgttatgc cgacaagcgc cgctgccagt gcgttcctga ttttacggat aagcgctttc
    35101 gtaccatcac tcagcttcgt ccagaacgtc ggtacctcag cattagcctg ttcattcata
    35161 tcggcaataa cagcatcggc ggtgaacata taaatgttac cgctgtcatc gtaataaccg
    35221 attttcttaa gcgactccca ctcttcacga atctgtgtac ggtccgcatc cggtaatgac
    35281 ctgaatgcgg caataagctc atcccggcgt ccgtcggtaa gctcgtcata agtgagcgcg
    35341 gaaatgccgt gtttcacacg tgcctcagcc gccagttccg cagcggcagc cacatcaccc
    35401 tcatcaagcg cctgctgcac ttctgccaca tcagcgaccg cagcgggagg taacgtctca
    35461 tctgccataa ctgcattaac gctggcctgt agcgcttccg cctgtgcttc cacgacggct
    35521 ttttcaacga ccacagggtc agtggtaact tctgcggcag gcgtagcctc tgtaactggc
    35581 gctgtctcca gtacagacag ttgtttagcc ttaaccttcg ccttacgctc agtcactgac
    35641 ttacctttgc gtgtgacact ggcgtccaca tcatacgtac cgtcatcgtt aattgcggca
    35701 atcactcctt cccgcgcttt gccgccgtta cgcggcctgt aaacaacctt ctggcctacg
    35761 ctccagtcgc cccctgacgc gcgtaagcca gtgctttctg cgccccgacc atctgttgcg
    35821 cgttgtcctg tttcacctgc aacgcctcgt tcgccagtac tgactgcttc actggctgcg
    35881 gatactgttg tatcactgcg tccggtgtct gcggttccgg ctcgttcacc actccccagc
    35941 gcaccagttg actctgaata aactgcattc gctgtgtcgg tgttcccccg tcccccgcta
    36001 agccctgcgc tttcagccct tgcagaagct gcaatatcat ctcccatgcg ggttccggta
    36061 gctgctccgt ctccggcggc aactcctgcc actgcaccag tgctgccagc gccagttgta
    36121 gcagttccat ttccatcctt tcgtccattg cccttcccct gtagtttcga caatgacgcg
    36181 gacgctttac cgtccggtct tacataaaca gtctcaggtg tatttacccg caaaccgcgt
    36241 ggtgtgcgtg gcaggctgcg cactgtggca tcgaacggct cacgctgcat cggtgtatac
    36301 gtagcctgcg gaacaacaga gtcacgctta tatgcagaca tcatcgcctg cgccagcggt
    36361 gcaacatgct ccatagactc cgcacctgcc caggacggcc gtgtcgatgt tgtcgccgcc
    36421 gatgcggtag ttgtcggtgt cgccgtagcc gtggctggcg ttgcactggt ctgcgcatcg
    36481 cgtgctgcgg catatgcttt aaacccgtct ggcagcgaca cgttatccgg tgtggcacgt
    36541 tttatcgcca ctttctccag catcgagtta accgccgact ctgacacacc cgctttagtt
    36601 gcagcatatg ccacgtcata cagactgtta cgctcttcca cagtcaacgg aatacccggc
    36661 gtttcgtaca gcttgtcgcg gtatgcagta agcgcctgcc ttaacttatt actgtcctca
    36721 gacgttgtcg gcgcagatgc ctgcgatgtg tcaccggata ctgcggccgc agcttcggct
    36781 ggcgacacta cctcactgtg tgttgtagtg tctgtctcgc cccgtgcagc tttagctttt
    36841 ttctccgcac gagcggcgcg aataccctga actgtatcga ccgtactgct gacggtactc
    36901 atcggcccac ctgcaatcac gcctaatgca gcagcttcac caacgccttc ccatacatcc
    36961 tcattaagcc cggttgccgt gttaacgccc gtattagaag cccactgtgt cgcgccttct
    37021 tcgacgccct ccacacctgc gccccctgcg gctttcagtg cacccttgcc gatcgcacct
    37081 gtaatcgtac tattcgctgc ggaacgcgcg gcagtacccg tcaacaaccg ccctgctgta
    37141 cggcccacca taccgcccgg accgagaaca cctgtagcag caccgatagc agcacctgta
    37201 ggtccggcac tacctgcggc cccggcaata atcaggttgc gtgctgtctc tgccgacaca
    37261 ccctgtttga tcagttcctg aaactccggc attttctgga actcttccat cggacgctgt
    37321 aaaaggtcca tactggcacc gccagccgcg tcacccccgg ataataaact acccatagcc
    37381 gcacctgctg cacccgcacc tacaggacca gccgccagga aaccccctgc ggatgcggca
    37441 gctactggca ccatagtcgg tgtctgctca acagccagcg acagcgcatc cagtggactc
    37501 tctaacgccc atcccactga cccgaggaac tccccattct cgccctgtaa ttcttcacga
    37561 cgcaattccg tatttctccg ggcgatggat ttttgcgcgt ccagtgcttt aaccttcgcc
    37621 agcgtctctg cctgttcagc gacctgccgg gctaactctt catccagttc cgctatgcgt
    37681 cgttgtgcat acgcgtccgt ccttccggcc agtcggtcac gctgccactt tatagcggcc
    37741 atcggaacag agtcaccaag cccggtagcc acctggtgtg cggaagtctg catacctcgc
    37801 catgtatccg ccagttcatt ggcgtcatct atctgctttt ttagcgatga gatattcgcg
    37861 ccaagcgtta actcaggaat catctgcaac ttatagtata agtcttcgtt atcctgatac
    37921 tgcgaaagga acttctgata ttgcgtctct ttgatatcat catagatttc ctgccgacgc
    37981 tctaatgacg cattctgcca ctccggtgct tccatcaccg cctgaaaatc acgtagtgct
    38041 gcgttatata cggcttcatt ctgtgcggca gcacgttgct gcaattcata acgggatggc
    38101 tgccgataaa tatctctcgt cgtatctttc ggtgcggcaa aaaggtcact cgacgccgta
    38161 gtcgtcatgg gattgaattg attaaacagg tcattctgat tgttcgccat aacattatgc
    38221 ccggtcgatt aaatccagta agtcgttatc aaggtccatc ggcaagtcat caaaaaatgg
    38281 acgctcgctg gctagcgcat tacgcgcctc acttaccctc gccagtgcgt tagtaagcaa
    38341 cgactgcatt gcaggggaat gcccggctcc gaagaagccc atcatatgat tccgctcaaa
    38401 atcattctcc ggtgcggcaa gcatcggtgt gccacgctga ttaacaagct gaacaggcat
    38461 ctgattaccc agtaattcca ccatagaatg acgcggctgt ggcgcggtac tggacggcag
    38521 actggcccat acgccaccca gcttatttat tgctgcggca tagtcaccac gtttcacatc
    38581 ctccagtgcg ccgttctccc gtatcagttc cagcgccatc cggtcctgtg tctccgggga
    38641 aaagtcagtc acacccagac gtttcgccag actgtcatat gtcggcttaa ttatctggta
    38701 gcgccctgcg gcagatgtac ggttctgctt accatccgtc tgtgtgaagg tgtagtactg
    38761 gcccggatgc gcgtcataac caacggtagt atctccacca aatgcgacgt tatacccgtt
    38821 atcgccctta cctgcggtac cttcggcatt cgccagtaaa tcaaaaaagc cccggaccat
    38881 cgggttctgt aagtcatcca tatcagtccg cccatgtccc gtagattcca tagcgccgat
    38941 tcattgcatc aagatatgag tctgacgcag tgggcctgtt agtcgcaata ggtgtggacg
    39001 atacaggcga cgtagtatta gcagtactcc cttgtttata cctgtccgga ataccgatac
    39061 ctgtggtacc acccgttaca gacggcgtag ccccggtgcc agtacccaac gcattattaa
    39121 tccctgctac cgaattagcc ttctctgcgg ccatctttaa tggctgcaac gtattcgcat
    39181 ctatcgcttt ctgtacattt aagcgcgcca gagcatccat cagattatgt gagtattttt
    39241 gttgcgccgt acctttcgcg gcgatttccg cagcattccc cctcaacccg gttacccaac
    39301 tgttcactaa ctctggtatg tgcggatacc cggcattagc ctgttgtata gcaaagtccg
    39361 acgaaaatcc tgcctgcgcc agattgcctg gctgatacgc tgccataagg tcacggttca
    39421 tattactgac atcggtttga tttctaacct gctgcaactg gtcaaggtag ctgtcctgtt
    39481 taaaccagtt cgacaactgc ttcgcgtcat tatccagcca gccgcgctcc acctgattcg
    39541 cctgattcag gtcgttccag ttacggtcga tcgctttctc acgcccctca ataaacggcg
    39601 taaataaatc cacaggcata aaaaacatta ctcacctccg ggttcttcca ggcatgacct
    39661 gaacgcggca cgattaccca taacaccctg accaaacagc cagtcaagca ggtagcagta
    39721 cgtctcattc gccctgtccg gcgaaaccgt aataccgatc tcgtcgcaaa aatgaaatgc
    39781 tgcatgggcg cactcatgta ccagcgtgtc cagccctccg tcgaatacgc ctatagcata
    39841 tacctgctgc gtattgcgca caaacttctg acacacaccc gaacacccca catcccccgc
    39901 gtcatcgaca ccgagtatcc ggcacatcgc cacccactgc tccatgtcgt acgcatacac
    39961 aaaataacca caccggaaca gcggcgcact gtcgacggta tgcaatcccg ctttccgcat
    40021 cagcttaagc tctttcactt atcacacccc cgtacccgga ttctgcgacc cgatattact
    40081 accgatggag tcatgccctg tataccagac cgactccata cccccgccac cgccgatata
    40141 tgcaggtgaa gaactaataa aaccaaacac gccattctct ggctgcccga ccagatacgg
    40201 gctgttccgg cggtcacgcc gcccctgcgg atatcgtgtc tcattacgtg tggaaaaata
    40261 ccccaacgcc gtcatcgcac cctgtgcggc attacctaac atcttgccct gattgttaaa
    40321 tgcatccgct gctgcctctg catacctgac agcatctgtc gggatgtttc gcccacggtt
    40381 cagcacctgc tgtcgacggt tccagcgaac atcatcacgc gcaatctgac gcccctcttc
    40441 atagcggtat gcgaagttac cggaatcacc cgccacctgt gaccgcgcca gcgccacatc
    40501 acgattgagc gacgggtcag gacatatgcc ataccgtccg tacaggtcat gaatgcgtcg
    40561 ttctgccatg ccaaacgtgc ggtcaacctc gttcatataa tctgccgcct gctggtcatg
    40621 cttcgggtta tacagtggtg cgctgcacgc ctccgcaact tcctttcgct cgcagggttc
    40681 aaagatttcc ctgaaacggt cccagtcacg gcgtgccatc ttgtagtaat cttctgcaat
    40741 gccaagctgt ttttcggcaa tatctatcat cgccagcgtg ttaaacgtgg caacagcaac
    40801 tgccgcaagt cgcgcccatt tggcattact ttttaccccg gtatcagtga cctgcggctc
    40861 gggccagaag ccatcctcgc caccgttctt cttcgacata cccaggaagt tctcaatctt
    40921 ttcgatagat tccgttttca cttcctgaga cttcaccatc ccggtatccc agttacccgt
    40981 cgccatagca catgcggcca taatgtcgca cccccttagc caagaacttt aacaggttgt
    41041 ggtttcggcg gtatcacctc cagttccggc gacggtaacg gatacgagat acccatacgc
    41101 tgcggacgtt tcaacggctc acgtggctgc atttcacgac gctccattga atacccgaga
    41161 taccccatcg cccccgctgc ggcattcgct gcctgctccc cgagacgccc gaacataccc
    41221 atagcgaaac cgctgaacgt ctgtgcccgt gccataatgt cacgaccgcg attcagggct
    41281 tctgcccgtc gtgcccagcg ggtatcgtca cgggcatcct taatcttctc ttcatacctg
    41341 cgtgcgaagt tcgcagacaa cgccagcgcc gtagcctctt ccacttcacg gtcacggaca
    41401 atcgcagcgc gcttacctgt acagtaacgg ctgatacact gcaactcccg ttccgtcttc
    41461 ccggttagtt catgccgcac tgatgccttc gcacgaccga cctgtacatc cttgtgcatc
    41521 gcatacagtt cagcactaca ggcttcctga atctctgtat tctcgcaggg cacatagacg
    41581 tcatagtaat actgctgccg ctccttcgcc atttgcaggt acttcttcgc catcttctcc
    41641 tgctttctgg cgatgtcgat agcagcctta gtgttaatga ccgccacggc aatagcagct
    41701 atctgcgcaa tgcgcgcaga ccatgtgagg ccgtcgtcag ttacaccttt tcccggctcg
    41761 cttgccgcaa cctcttcttc gccctctgcg ccttcttcac cttccggtgc gagttcttct
    41821 tcgtctgcca tatcacacca ccagtttcac catctgataa ctgtctgcgg catctgtctc
    41881 aatgccctcc ggtacagggc gcgcgtcaca ggctatcgca tgaatatggt taatgcccat
    41941 cgctggcgct atcgcagaca gatagtcaaa catctcctta tccacgccat catgctctgc
    42001 gtaccagcgc tcaacctgca ttactgtacc cgccagaaac aacggacgga acacataaac
    42061 gaacataaag cccacaggcg tgtcaccttc aaacgccata ataaccataa cgccccgctg
    42121 ctcccatgct gcaaggaacg ccggaacatc cagaccgtgt ggctgcccgg tgcgctggtc
    42181 ttcttccgcc caatgccgca ccagtaacgg accaatcact ttcgtaaaga accgctccat
    42241 ctcatccgca gtcttcggat actgcataat cttacaactt gccatcatcc cctcacttaa
    42301 ccgcttcctg catcgtcgca cccagccgca accagcgtac ctcgcccgtg ccttccagga
    42361 taatgcggtg ttctgtattg cgcattaatc gccgtgtgcg gaacggacgg ttatgcgcga
    42421 cagggcgcga atacacttta tgcggccccg acttaatcat gtacgtgacc gtgccatcca
    42481 cacctatctg tgccacaggt agccagctct gaaagcctgt catgataacc ccgctgaccc
    42541 acagataagg tcgtagttta tccccggcat tccactggtg tagcttgccc tcgtcatcca
    42601 gcatcagtaa ttcacctgaa tcactaagca ccatatccac tggcgtatcc gagaacgtgc
    42661 tcattgcagc aacctgtgcc gcagcactgt acgactgcgt gtccagcaat aacataaacg
    42721 acactgcatc cgtcacgcaa aacagataac ccttatagta tgccagtcgt gtggtttccg
    42781 gtcgcagctt aatccagtca tcagcggcaa gaacaccttc tgtcagaagc cgggtaggct
    42841 gcgactggtt cagcagtacc agaccatcgg tgctggcata taccataccg aacggcgtca
    42901 taattgcgga atgcgcatag ccgcatccta tgtccggcat cggataatct gcctgtacca
    42961 cggaacgaca cgggcgctca tcgcagacca gtgacgcgtc caccacatat gaccgccccg
    43021 tggtggatac aaacaggtta ccgtcatcct gcgcaatatg aacgatgttg tcatccagcg
    43081 tcatctcaag cgccagcggc cagttccacg gctgtccgtt cttcgtgaac aacagacggt
    43141 tcccgacaaa acctgccagt acagacgtac cgtcaattgc cagaatcccc cgtagccccg
    43201 caggcggctc ccggacctca cgtgtcggta actgccagcc caggtccata tccttacggg
    43261 tatccacata ggtcttctgc gtgatatcaa tctcgtcaac cagcaggtat tccgtgatga
    43321 tattctgctc ttcctcggca ccggaccgca accccgtcgt cctccggtaa atacggacct
    43381 tctgcacccg gtattccgca ggctgcgcct cccagccaga caccgtcaca gactgcccgt
    43441 cattaaccgc cagttctgta ccaggataac tgggcgcact ctcttccgac aaccagttca
    43501 catacgtgaa cacatacgtc ctgaatgccg tctcaatatt cttatccggt accgtaatgt
    43561 atgcgaccag cggtgtggta tctggcgctg gcacgccaag tctggcatag tccagtgtac
    43621 aatctttacc aatggcagcc acttccggat atggcgaacg tcctgtcagg tacaaccgtg
    43681 gcacatccgg cagccatcgt gcgacagaga cgcaactatc ccacgcgaaa taacaacaac
    43741 catacgcttc aaccgtcagt gttgtgtccg gtagctgcgt gacaaagcgc ggctcccgcc
    43801 acggccgaag cgtcccatgc tgcaaattca catcacgggc caccgtcgcc ataccttcgc
    43861 caagcatatg ctccggcgtg cgtggtgata tgcccttaaa ccctttcagt atcatgtgcc
    43921 gaccccgacc accgtacact taagaattgc ggcatgttgc cccataatct ccggtccacg
    43981 caccgcaacg gtatgcggcc ctgccgggat aatgcctggt gtgataccac gtactccggt
    44041 gctgaccatc gttcccggcg ttcccgacgg catcagcgtt gtcagtacac gcggcacatc
    44101 actcccgtcc actgcgatag ctgttcccgt aacgttctcc gccagtcccg gtgtctgagc
    44161 ggtggccgtt ccaagatacc cgatatactc aatatgcagc cgaccaggta cttccgtggt
    44221 gaagctcatc acagcagcct gacttggctc tcctgctgcg tattgcgcga caaacacatt
    44281 cggctctacc ggaacagcag ccttctccag cttagtgatg gacccgtagg cattaatcgt
    44341 cacactgtaa ttacccagcg tgtatgtgcc agcagtcagt gtgatgccgc gcttcacacc
    44401 ctccacacgt ccggtatcat ccacagataa cgagtagcca cccattgtgt aactaccctt
    44461 aacgccctcc acttcttcca ggtctatacg gtcaacaccc ggcccgacag tgacaccctt
    44521 agtaccgtta ccatcgataa gctgccgcaa ggcgtcaccc ccggcatcct cattatatcc
    44581 gacaactctg ccaagactgt ccagttccag tgccccgaac gtccctgcac ctaacggact
    44641 gttagtcagt ttcaggataa atgggtctgc ctgttcacca ctacccgaaa tagtcagtgc
    44701 atccggcgaa ccagacgtca tgaaaatacc gtcgccatct ccggcgacta gacgaattgt
    44761 tagcgggtta ttctgcgtgc catcacccgt gacagtgacg ccctcattct gccccaggta
    44821 caacttcgtc atcagttgat taccgataag ccgactcagg ttagctgcat caggtgacaa
    44881 cgtaacgccg ccttcaccgc caccctcgcc acatggagtc gctgccgggg cacacggcgg
    44941 cggggtatac agcggcaccg gggcttcccg cgcatcaacg atacaaccat tgacaacatg
    45001 cacctcgccg taccagccat ccgggataac aggttgtccg ttcagcctta cgcacgcgcc
    45061 atcgtacgac agcgtacgtc cgaacgacag acacacactg aacgggtcac atagcggtgg
    45121 cacagcctcc acaggctcag gtgcacatcg cgcattcttc ggtgtcttgc ggcatttatt
    45181 catcgtgtcc gtctcccgtt aaaaaccgtc tgcatccgta cgcgtcctgt gcggccctgt
    45241 aacaacctgt cagttcccgc tgcggcaatc cgtcgatcga actcttttcg ctgcgctgta
    45301 gccgatacca ggctcgtcca cggacgtctt tgtaacccat acagttgtgc gcgtgcgcca
    45361 gccaggactg cttctttgta gcgcgtcagt aatatctcat ccagttcgca cgcgtcatga
    45421 tgtggtgcca ccgacacaat cacagtgata tcctgcggct caacgggtac aggcttcacc
    45481 catagctcgt tcggctgctt ccagaccaca tacaggtcat caaactgaca gaagcctgcg
    45541 caatccgcat aagcacggtc gccgtttttc ttcaccttat gcagtgacac gatacgcata
    45601 caatccggcg cttccagaat gtaatccggc acacacgact gtaggcatat cgtaatctcc
    45661 cgctgtagtg caagtgaccg ttccgcaaac tcaatcgcgg cattgcggac ataatgtgcc
    45721 agaatatcat ctgacaactc cggtatctcc agagacaact ccggcaggaa cgcatcaagc
    45781 ggcaccttcg gatatgcttc aaatactacc gtttgcatta cgccacccct ttggttcgct
    45841 ggcatccttc tcgctcctat gccatacaac cgtcgctttc tccgccacgc ccagcacgtc
    45901 gaagaacgct ttatagtgca tctgtgcagc attcccagcc gctggcgaga cttccgcatc
    45961 aatacttctg gcacgccata acacccactg cttaaccgca gtcacatatg cgtcgtcgac
    46021 ttcactctga atactgtccg catcagggcg cactgcgcac tcaaccagaa tatacgcctc
    46081 ttcatgtggt ggcatttccg gccacacata caatgcatcc gtcatcgcat ccagtgcata
    46141 actggttggc ttgtacttac ccgttgcccg acgacacgac gtgccacgcc actggaggct
    46201 ggactccagc ccctgtttgc gcattaccga caataacctg ccatccttcg tggcgtgccc
    46261 aagcacgcga cggatagcgc cgcaatcgca tgtgtcctga atgacgctgc atggagtcac
    46321 aggaataacc tttttctcca taaacaaatc cgggcgtttg tcgaatacca ggttaacccc
    46381 ttctgcgacc cactcccgga tttgctgttc gctccacgtc tgattgtggt agccgtcttc
    46441 attgtcattc aggtcaccgg atatacttaa taccagtgcc tgtgtaatca tcagctatcc
    46501 ttcaacgcgt catccagtgc tgtgtcaaag gtttccccgg ttccggtaat cgctgcatca
    46561 gtaactggtg tctccggttc tgatactgct ttctcagatg ccttgctctt atcttttttc
    46621 tttgctgcct tcgccgttgt agcatcgcca cgcatcgccg ccagttcttt tttaaatgac
    46681 tcgtactcaa cagccatatc atcacgcatt gcctggcgtt ccagttcaat ctcttccagc
    46741 attttcgcgc gaatggcagc aatctcgtca gacaatgacg ccttcatctc cgccacattc
    46801 cccggcggta aatcacgcag acgacgctgg cgttcttcga cgatggtaat accctgcgga
    46861 tcataatcag gatttgcatc agcggggttc atggaccctt gcaggttata acatgccacc
    46921 acaaggtcag aacgctgtgc cagtccgtca gagaacggat gaataatccc ggttgctttc
    46981 tccatcagat atggaggacg gcctggaatt ggcttcggca tcttcgtcgc cagggtatcc
    47041 agttcagcct gacgctctgc caggatacct tcattacgga tatccgcttc gtcatgtgta
    47101 actgctgtaa ccatatcaga ttcctacctt acctttaggg gcacgaagtt tagtaatggc
    47161 ctctggacca cgacctttgg atgtggaccc cagcttaccg gctttcagct tcgtaatcgc
    47221 agatgcatca cggcctttac caccatgcgc cagcttacct gctttcagtt tagtggatgg
    47281 cttaccttta ggcgttttcg tggcatcacc gttcaaacca atctttttca taatatgctc
    47341 cgaaaatggc aggcagcggc ggggataagg ccgccacccg ccgggacgct tacacttcgg
    47401 tcgggacgtc gaagccctga accttagcta ccagttgtgc cttcgcggtt gtggtatgca
    47461 gcttcacatc cttatcagtc ggcaccgcgt ttacccgcag cgtcaacaca agggttttac
    47521 ccttcggcac atagtacggc tccgccagat gcccatactt ctcaaatggc gcagtaattg
    47581 ccgtattggt aaacaactta tccagcaccg tatcaggtgt gtattgcttc gtcgtatggt
    47641 catacaacat gccacttgga cgtaatgctg cacctgccat acgcgcatca ccttccagta
    47701 cttcaaacag aacaccagtc agtaaatgct gttccggtac aacgtacatc atgatgtggt
    47761 cgttcagtcc ggtttctgca ttcatcagtt cctgaaactg gtaattcttt tcggatggtt
    47821 gcaacggcca gcccagcgtg aaatacccgt tcgcatattc gaacgcgata cgacgcgcat
    47881 gaggttcagc aaactcatac gcgccattac agttcgggta gttcgggttc ggcgtaccgc
    47941 caataaataa tttcgcatct gccatgatta atctccgaag gcccaataac caacagccag
    48001 cgcatccggg taaatcgctt taccacccca tgcagcaagc atctgatact ccacgccgaa
    48061 gttacggctg ggttccacca ggcgtccttc gatgatgtca gccgcgaaac cgaacgcatc
    48121 acgatgccct gcaatgatgt aataggtaag ctcacctgtt tcatccacaa atgccggaac
    48181 atgcgaagat tcaatcgggt taaagccaaa cagtttatgc ggcataaagc cgtcaatatt
    48241 ctgtgagcaa ggcttacagt cacccatctt acccgcgtcc gcatacggcg atactgacaa
    48301 cacttcattc agcgcagtcg gaacaatgac aaacatcttg tcttcttccc aacgctggcg
    48361 gtcacgcagt acctgacgca gacgtgcaaa ctgacgcgta atattattcg gcgtaaccat
    48421 attcggtgca ccagactggc ccagcgaaat gttatgacga atacccgcct tattgccttt
    48481 gttacccgga tcagcttcaa gcaccatagc attcagtacc cagctacgcc acatgtcagt
    48541 aagtgactga tatgtagaat ccaggaatga tgcctcccag gaatcccact gcttgcacgc
    48601 acgcgcgata tccagtttgt caaacttgat atcctggtat gcggcattac agatttccag
    48661 acagaatgac tccggcgtta actggttcgc aattaacggc tggttcttgt catacggtct
    48721 ccacggtcca actttcggct ggcgcgtaaa ctggacgatc tggttgcatt gcgtaatgcg
    48781 ctcgttaatt tccgagtttg tgatttcagg aaggaaatct ttctcccatg cctgcgcaat
    48841 gatttcatca taataaccaa tgcgagccag cggcgtggct tcaagaccgc cataacctga
    48901 cgcagattta tacatatcag cactcctgat tgcttactca cgccgcacta gcggctacct
    48961 tttgcgcctt gtatttagcg cgcaattcct tgtattcctc acgagtgatc tgcccggtag
    49021 tcagttgccg attccacaac gatacatctt catctgtgta ctgttgttca tcaactccac
    49081 tcgccggaat actggcggta gtcgcacttg aaacactggc aatatctgcc agcgacggct
    49141 taccaccctt aaaatcattc attgcttcga taacgaaatc cgcgttaccg ttgttatatt
    49201 catcacgcat aagttgtccg agagtgaccc gtgacccctt ccaggcagga cgcgatgaaa
    49261 attgtgcaaa ctgttcagac tggaggactt tttcaaagtc aggatgtgcg ccaagaatcg
    49321 cagcattcac gcgggaacgc tcgctgctct tctgcgcttc cgtcatactg gcatcgcgct
    49381 cctgtcgctc tttctgaatg cgagaaagct cttcgtgcgt aatgcgcagc ttatcctcgt
    49441 atcgcttctc cagtctttcc agcgcgggac gtaaaacttt cgcagacaat tccgcagcag
    49501 cttcacgatc cagtgtttca aactcctgat caacatccag aacctgctgc atttccaggt
    49561 cttcacgata cttccgtagt tcagccagtt ctttctgcac atcttccggt agctccagtg
    49621 cacgagactg tgctgcggca agctcattac tctgttcact aagacgctct cttagcgtgg
    49681 taacttcccg ctccagttcc tgaacacggt cactattcgt cgccagaata ttcgctgcat
    49741 ttgtcggtgc accggactct gacgtgacct gtgcggaaac ttccggcgca gacgcctgcg
    49801 attgttcttg tcccgattca ttctttttgg cctcttcggc ctcctgctcc cgtcgtgcct
    49861 gtaccgctgc tgcggcatga tgacgacgtg gctgcatgga atatgccata atccccacct
    49921 cagatttctg tcagtcgttt cagtaaatcc agaacttctg caagcgcgtc aacttcgccc
    49981 atcagccgta agccgttatc acgggccgcc ggactcacaa gcgctgataa cgcgacctgc
    50041 tgtaacaacg cccgacgatt tgctgcatga gtctccactg cctgaaccag agccgcgtat
    50101 gcagcttcat tgcgaggcag cgtcattaat aaatcagcct gtggtgtctc tacagtaatc
    50161 atcggacgtc ctgttacttg ctgttccagc gcaatccagc gatacggtca cggataccgg
    50221 gacggctacc ggaagattgc ggagttcgtg ttgatgtctg gtttaccggg cgaccgccct
    50281 gcgtagtgcg cagaggtctg cgcgacttac caccacatga agcacatgcc ataactggca
    50341 cccccatcta ctatgcgcat tgcgcgttac attttacggc ttattgaatg ccggaatcaa
    50401 atatccgcca atatcaatcc acttatctgg ctgccccagc aacgcggtac gaccaccatg
    50461 aatcgctgtc ggcaactcag cacctttcgt caccgcctta actttcgtcg tttcagcgat
    50521 aaccagcttc agcggctgac ccgtaacacc tgtacccgtg atagtggtac catcagtaac
    50581 gacctgacca ccaatagaat ccaggttaac ttcccaggac tgtggaccac catcattatt
    50641 cagcttcgtt gtcagtttca gcgtatgcgt atcttcgtca tacgtgaaac cactgattac
    50701 gtcaagtgcg ggtaatgacg caatggcctg attaatcgcg tccgtcacat cctgacagct
    50761 gggaatgcgc acaccaggcg cacgtggcgc accgtcgcag tcctggaata cacctgccag
    50821 aacatcctta tcagccacaa tagcagcatc cacaatttcc cggatactgt ttgtcagttg
    50881 cttaaccaga tggtcatgcg ctgcggtatc cagcggaacc tgcccactaa tagatgtcgt
    50941 accgctcagt cttgtattac ttgcagacag attttctgcg gagatattaa ccaggttatt
    51001 cccggtaatt tcccaactag tcagtgcgct actgattaaa gtatcaacct gtttcttaat
    51061 aaggtcagat aatgcggcat gtaataacgt ttttgcaccg cctaccagtt ccagtgtccc
    51121 actaagtcgc gcattctcaa tgtccacatc acgtaattgt atgccatcaa gaccattatc
    51181 agtgacagca tcaataatat gtttctgtaa taaaggcccg accgcgtccg cgatagcctg
    51241 cgaaactgat ttatccagcg taataccgcc cgataatgac gcaccagaaa gtttagcatc
    51301 tgctatttta ttctcagaaa caatttccag aacatgcgtg gttaattcag gaccaacagc
    51361 atcggcaata cttttcttcg cagcatcatt caatgttaca gcgccttcca gtgctgcggt
    51421 aattagtttc gccgaatgta attccgcatc gcgcagcgtt acatcattaa atgaaccacc
    51481 atgaatctct cgctcaatac aatctgaaag cacagcacac aactgtgctg acagcgccag
    51541 aagcgcaggt tcatccagcg tgatactacc tttaaccgat ggtgactgta attcaccatt
    51601 aataatgcgc ggctccagta aagtaccacc ttcaacaaga gacgatgtac agcaattatt
    51661 tgacattatt ccacccctaa atacatacca tatgggatat acccaagttc ctgctgacta
    51721 tagcgggtca atgaaataaa cactgtaccc acggctgcgg catcatttaa ctgcaatcgg
    51781 taagtgccgg gcacggataa cgcgactgta ttattacagg ccgataatgc ccataatcca
    51841 cactgcgtaa cgtcctccac atatgaatat ttcggcacag aaataaggcc ttcatcggca
    51901 gcacatgctg aaccatttgg aaatatccct ttatcaaatg ccattttctg cacaatggct
    51961 aattgcggaa tattctttgg ctgcccttct tctggtgcct taatatattg tgcaaagccc
    52021 caggctgaaa taatagccgt acatcccggt gacacaacaa atgcacggct atattcactg
    52081 gtactcgcgt catcaaacag aatatcggta ttcatctcag actcctgcca tatacgcaaa
    52141 cgggagttgt tgataacccg gcaccggaat cagttcgacc tgcacatcac caagatgttg
    52201 tgtgtcattc agcaccagta aatattcacc aggcaacgca ataagcagtc gatcattatc
    52261 cttcgtcagt tgccacggct taccgcccag cttcacaggt gaacgaaaca gaatattcgg
    52321 cgcactacca ggcacaatgc tgttacccag cacactaccg ttacgacaac aaccctgacc
    52381 gcccggtgcc ggactagtac ctacagaaac gccttccacg gtaaacacag catcttccgg
    52441 gatattgtat ccaaccagta acacactgct tcccggacgc acagaaatca gtgcggattg
    52501 cgcataagta ctggtcgggt cgaacaggac catatgcttg cctgtcacag atttttctgc
    52561 ggcatgtgcc ccacggtcgt catataaacc gcctgtatca atacccataa cattatgcct
    52621 cagaccagcc accacacaaa caccgtcgca ccaacgccgt agataaatcc acgaagcccg
    52681 gcacaacacc aacagtcagg aaacagcgta ctggcgatac gccagcgaag cgatgtctca
    52741 ggcgcgttac acggattaat cttgtggatc cacgcttcca gtttaccccg cagcgtactc
    52801 gcttcttcct gcaactcctg cgcttccgaa gccacttttt ccttaagctc ttctgctttt
    52861 tctttcaggt cactcattcg gtaccgcctt aattccatcg ttaagcatgt tacgcaaaga
    52921 cttcgccatc gtggtgaacg tatcccacgt acggtcatta tctacaggct caacaataac
    52981 agggttcttc cagtacacac ggcgggagtt acccacataa ccacccttag aatagcgcaa
    53041 tacgcgccca ccacccagat caacaacaaa tgacatatca ggctcaacct gagtgattct
    53101 gccatatccc tgctgaatat cgtaaacctg tgcccccaca tatggggcta caccattaat
    53161 cattatgctc cctccgcagt tcctgattgt ggcatcatct caccaccacc ctgttgttgt
    53221 ggccccggta caccctgctg cggcattaca gggggcgctg ccggtatagc ctccagttca
    53281 tctaccggaa caccagacgc tttcagcgca ttctctacag cccatttcat aatgccaggg
    53341 ttaatcgcac cctgtgctgc tgaacccatc gtggcaacca gttgcagcgt atcaaacgca
    53401 ttctgcttcg caacttcttt agaaataagc cccgtcgcac cctgcgcgat aatcttcgca
    53461 tcacctttga cactctcatc gtcggaatat tgcatgttgt agttatacat caatgttcca
    53521 agaggtctga aaatccccac atccatgttc ccgactgccg actgtatcgg ttttatcgca
    53581 ttcccgtaca gcatcgccat cccacggaac gtgcggttcg cccctgttcc cacaggttcg
    53641 ccgtgaattg ccgcaggtat ctgcgtcaca cggtctgcca ggtccataaa aaactgtgca
    53701 caattcagat atgcccccat gttcgaaggt atatgctgca atttataggc cggatgaccg
    53761 ccagcggaca tgtccgggtc gaccggatgg agcgttaatg ctgtaatacg gccaatatct
    53821 tccggattca tataccgctg aatacgggag taatccacct ctccaaacgg cccagcagac
    53881 aaaccttcat ttgtcatcaa aaagcgtaac gtattcatat acgcacgctc aacgtcacgc
    53941 agcttctggc aaatactgaa tccgggtatg cggtcagcag ccgtctcaaa gctggcagta
    54001 tacacaggac gaatgcctac gtgcggattc ggtaatatga atgcctgaat cgtatactga
    54061 cctaccatac tgatacaggc gtcataaaac tggtcatcgt caataccagt aataccgtac
    54121 tttttcagtt ccttaccgga gaacatgccg taatgccgca gtacatccag cgtttctccg
    54181 ttaccccaac cactgccgat ggtgtccggc tgctcagggt tacgggacat ccacttaaga
    54241 ttctgtgtct tactgattgt cgcctcaagc accttctgca cattcgcctt tatgtaagac
    54301 ttcatgccca ggcacttgta caagtcctgt ttcgtcacct gttcccgaat gaaaatcccg
    54361 gtaccctcct gcgcattgcg cgaatcaggt gaccagtaga agtcaaacac actaattgga
    54421 cgaaactgaa actggatttt atctttcggc ttcaacgaag acccggacca ttccatctgt
    54481 gtaaccattg tcggcaccgg accgtggaat actgcgaatg ggtacgtggc aaagtcagta
    54541 atagttttca gaaacgcttc ctggaacccg ccctccatgc actggtcttt gattaaacgc
    54601 gacatgttat tacaggcgac ttccgcatac tggcgctctt tctccagttg ctggaactta
    54661 acggaacgta tcaggctaag caggtctccc tgaaatcccc ctgctgcata caattccatc
    54721 ttcaccttat tcagcacttc catacgtgct ttttccgaca aatcagggat tggcgtagga
    54781 cttatgacaa acggcagttc cggcgtattc agaagcaggt cacgcagcca ggacgtcagt
    54841 gcagagactt tcagtgccga tatgttcaca taaatatcaa tgcccagttc gtcagcaatc
    54901 tccgcatctt ccggcgacag aatgccttca cgctgttcat aacattcacg taatacagtg
    54961 cgtaacgact tcccgccaat ctgctcacgg gcacgccatt gcactgcggc attccagcgc
    55021 tgtgctgtca cgttcgccag cgggtcatgc gccttctttg gcacttcaac ttcctgcgct
    55081 tcttctctca tcacatgatc cttctacgct tacgcaacct gtcgcggaat gcgcgcttaa
    55141 ctgccgagac atcaccacta ctctcgtcat acctgagtgc gtataacgca ccatattgta
    55201 gcgcatctgc cggatgggag tgttcatttt ttaccgggtt agcggtatat gccacatcca
    55261 ccgtaccacg tatctgcatt ttgccaaact tataccctcc ggcacatgac gcaattaccc
    55321 gctcgcagtt cggactgata agcaagcctc caacgtcttt gttcagcaag cggttaaccg
    55381 cctcaatacg ggacttcgta ttattagtgc cagccacctg tgcccgaata tgtgcctcgt
    55441 tcagcgtcgc cactggtgtt gtcgccgtca gcgcattacg cgcattcgcc gggtcacata
    55501 tcgcaaggat accactggac tccttatatc gtgactgtac cagcggcaaa agccccccat
    55561 acacgaaatc ttcaaagccc gtctcttcgc catacaactc atccaagata acccacttaa
    55621 caccgataaa ctgccagaac agcgccgccg gatggatacc cgatgtatcg atagaaatca
    55681 tcgtctccag tccgggctgt ggcagtatca catgtttagc cacatgtatt gcgcgggaga
    55741 atcgcggcca tacaggctta ccacttctgt catccaccac cttgagacac aacaactggt
    55801 cgataccatc gtaatcacct accagcatct tactgtctat ctgtgactga taataccgga
    55861 taccaccttc aaacccgtca ttaccaccca gcggtacaag gttctccgcc tcggggttga
    55921 cctcataaga aatcatcccg tcatcagact catgcttaaa cgccgccggg ggctgcataa
    55981 acaacgcgta atcatcccgt tctgaagcaa acagattaag caaccagtgt ccacgcggcg
    56041 gcatgttata gtccataatg acaccacgcc atgagcacgc tccacgttcc cctgacgggt
    56101 aacgcccgat acgctccgtc gctgtggtaa acaccgatgc aggcacttcg gtcgcctcgt
    56161 tgatccagat agccgtaaag tcggtggaac gcagcttctt gatactctcg tcatcactga
    56221 ccgccaggag gattaactcc atatcaacca gcgtaccgtc atgctgtaaa cggaagcggt
    56281 agtgcccttc catcggcgcg gtctccttaa tcccccccgc ctcatgaata agccactgct
    56341 ccagtgtttt ctttgttgtc gtacgcaggt tcggatatgt ggcacggata acaccaaacc
    56401 gcgtatagcg tacaccatcc ggtgcaggct tctgccggat ggctatatat aacaaatcat
    56461 taacacaggt aaccgactta ccggaaccaa acggcccaca tatcgcccgt atcttcttca
    56521 ccttccaggc ggcatggaac ttactcgctg tcggatacgc ttcatataaa tgctgtctca
    56581 tgcgccacct tcgccaacat ctgctgacac gtcatacact gcgccacccg gccccatatt
    56641 tatggagaca gacacaacac tggcggcact acctcccccg tcatcacgcc gtttattcgt
    56701 accgtttgtc gccgggtcaa gctgcccaag ctgcgccatc agacgcagtg tttcccgtcg
    56761 ctcgcgtatc tgcgagacat ccgcaccctc cacaacatcc ttatatagcc gctccatcag
    56821 gtctgccgtt atcatctgcg cacgcagcgt aactgacgcg gtatcaccca tcgcgtcaat
    56881 acgctttttg aaatcatgca tcatgcgcaa aaacgctggt attttcatga gcgccaggca
    56941 ctcttccatc gtcataccat agttgcggca aattacttcc ggatttaaca gcataccgtt
    57001 gcctgtacca tccggcacac gacacataat taaatcttgc gcaatacagg gccagtgcgg
    57061 acgcagccgg gataaatcct tacgctggct gacttcgaca acgcggttat ctttctcact
    57121 catacgttgc ccacctctcc cgaatacact atactatcta atgtcaagtg gggcgtgcgg
    57181 atgaagcagc taaggaatac ctaccacgtc acataatatt atgtacgtag atgccaggat
    57241 atagacctct ccattgcagc cgtacagcca tgttgttatc ccaacgcata catgccaacg
    57301 taccgcctgt tccgcgcgcc ctacttgacc cctgaaaaat gcggcgtccc tgccactcca
    57361 ggataaagaa cgtcagttac aatactgaaa cacgctttac tcttgtcaac atttacgaac
    57421 ccactgtaat atcaacatcc ttagtcgcat taagcacgtt aatatcagac accttattat
    57481 tgatacggcg gaccataact ttcaggcgaa taaccgcttt acctgtggta ccacctttaa
    57541 acgacggcgt acccgtaacg acctctaccg gaaccagcga ccccttactg cctttcgata
    57601 ccagttctgc tgcacataca tgagtaaacg tcgctgtctg attcgccatc gtcagacgca
    57661 cgacacgagg gaatccagtc acatcctggt tatttgcacg aatacgaaag tccggtagcg
    57721 acacttcggt atcctcatta aactctgtca ggacacaact tacgtcaata tcaatagcat
    57781 acgcacaacg cagtccttcc agcggcagtg catccagtgg cacacgcagc accccggtgg
    57841 agtcacctac attattaaat gcagtcgtaa actctttcac gaacacctgc gtatctgcga
    57901 cattctgtac cggacagtta accatgacac gtttctggtc accgaagtaa atgtcgaata
    57961 ccgccgccac atgttctttc gacgtcagca caaactcatt cgccctgacg gtcttgccat
    58021 acggcgtaat aatacgacca tccttagtga cgaatttaag cgtggagtaa ccccgacgct
    58081 gcgcaccttc tttatcctga ccatccagca tgacagcgta atagacaaga cggatatgct
    58141 tagaatactc ctgctcaccc tcaatcgccg acaggtccac cgtgtagtcg gtgttctgac
    58201 cattaagcac aagcagctct tcacgcatat gctcatagcc tttagtcccc atcttcggcg
    58261 taataacttt aggtgtttcc gggaccatga actggacggt cttgttctca aagtcacgca
    58321 gcttgtagcg atcgacaagc acacctttat ccgtaatctc aatcgcgttc ggtttctcag
    58381 gcgtactctc tgccttattc agcgcgatga caagcgggtc agtcttaaca cccagcccct
    58441 gcaccttcac ggactgctca cgcgtgacat ataaatgtgc ggaccatttc ttcggctcat
    58501 gctcttccag caggtttacc gtcgccagat gcggcgtctt aacaacgcca acctcactag
    58561 gagcgatcgg attaccactg gtctccacac cctccgacgg catgtgtgcg gcattaatgg
    58621 tcggcagtgc gctatctttc aacttcataa ctatcggact ggctttactg ccgtggccct
    58681 caaacgtaag gtcactacgc tccgcatcag ggtagaacga cgcttttaac gtgccagatt
    58741 cattaataag ctccgtatca gacgtcatag tgtcagggtc aaataataaa ttatcattgt
    58801 ccttactgat attaagaccg actttcagaa caccatgatc tgcatcacca ttaaacgcca
    58861 tatcagtatc aataccgaaa ccgccacgaa catcaaatcg tgccgccagc gcaccacctt
    58921 cattatataa aatgttacca ggcgtctgac ttaacggaat aaggctcgca tcaataacgt
    58981 caccagtacc cgccagctta tgcgcctcgt ctttatatac gacaacttta accgtagtct
    59041 tatcgctcat agtaagctct ccatgtaaat cctcgtaaca ctacataaca ttatggtgtt
    59101 gacttaagga ggcgaccacg ccacgatgca aacagccgta acattatgtg tgcgtataac
    59161 gccacaaaca cgttctccgg gcaagaaaaa cccgcgcatt gcgcggggta tgggaacaac
    59221 atgtgccata aaaacgctaa atccctcatt gacaatagta ccttaatgaa cttagcgctc
    59281 acaaggctat tgtcatttac agcacatgtg caacgggaca aacataaccg ttatggctgc
    59341 aataaaggaa gcagggcgtc tgactggctg gtcaccgtca gacctctgcc tccgccccat
    59401 ccaggtcccg taaccgcgtc acggcgaatc ccggcattat actgataacc acgttaccag
    59461 ggtcatggcc cctccctttc tccaccgaaa acatccagtg ctggtcatgc agtaacgcac
    59521 gcataaccac cccacctgtg cctctgaaat atgccgcatc tgacggagat accctcaaca
    59581 cgtaactggc ggcactgcca cccataatga tgtcgtcctg ctgcatttct cttaatcgtc
    59641 cgtacatttt cttttctctc tcaaacatgc ttaccgttcc ctttcagaac ggctatgttg
    59701 ttatcccggt aacagactgg cgcgttatac acgcgcagtc aagccccggt cccgttgcac
    59761 agtttcacat gtacaacggt ccggcatgtg tgacacaccc tcccggctaa acaaaattca
    59821 tttatcacgg gccgaaatca ttcggaatta tcttagggca gcgttcgggg aaaacacgga
    59881 aaaaattttt tgactggtca atcagacgtg aaaagtggag atgacagagg gggtgccagg
    59941 ggctgcgcaa taacgacagt gcgcatggtc attagttaag taatagtcgc aggcacaact
    60001 aacgcgcaat acgcagtgcg caatgctgat tgtgcgcaaa gactataagg cgggtaatag
    60061 tcgcgcaggt aactaaacgc atattacgca ctgtacaatg gagtgtctaa tgcgcagtgc
    60121 gcgcgcagaa aattggtcag tgtcgggtgc aatacagtaa ggagtgcgca gtgcgcgcga
    60181 ctctcgccgg tccagacccc cgccacccct gccgaagcgc cgctggtcgg accagtgccg
    60241 aagcgccgct ggtcggacca gtgccgaagc gccgctgcta agccgttgat agtaaaggct
    60301 tttcactctc tccatttatt tttctgttaa atttccgtta ctacctttgc tgacacaact
    60361 ttacgtatcg gaactttcag cgctaccgcc accccgagta agccctgtag catacacaac
    60421 tttacgattg attattccat aagctgaatc agtacagaag atgcacaaac gcgcaatgcg
    60481 cagctagtgc gcactatttt cggctatagt aagttattga tttataagga gcgccggaaa
    60541 cactgctatc tacgcgcatt acgcaggcca aaaagttatt tattttcagc aacttaaaat
    60601 cgctgttgtt tgtcagatgt taaaagcctt tattatcagt gtgttggtca cagcacaaaa
    60661 ttcccgagta aaaaacgagc aagaatttta tcctgcgccg cccggcgcgg cgggtgaaat
    60721 gcggcatata tattattgat tttaaagaaa taataataat aataataaac ggggaaaaac
    60781 agaag
//
LOCUS       DAZFRN010000007        57858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFRN010000007 DAZFRN010000000
VERSION     DAZFRN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.007"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..221)
                     /locus_tag="ACHKUB_000429"
     CDS             complement(<1..221)
                     /locus_tag="ACHKUB_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6653504.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDS"
     gene            229..730
                     /gene="tnpA"
                     /locus_tag="ACHKUB_000430"
                     /pseudo
     CDS             229..730
                     /gene="tnpA"
                     /locus_tag="ACHKUB_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(748..1416)
                     /gene="ydcL"
                     /locus_tag="ACHKUB_000431"
     CDS             complement(748..1416)
                     /gene="ydcL"
                     /locus_tag="ACHKUB_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO6653505.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1719..2312)
                     /gene="tehB"
                     /locus_tag="ACHKUB_000432"
     CDS             complement(1719..2312)
                     /gene="tehB"
                     /locus_tag="ACHKUB_000432"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO6653506.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2309..3301)
                     /gene="tehA"
                     /locus_tag="ACHKUB_000433"
     CDS             complement(2309..3301)
                     /gene="tehA"
                     /locus_tag="ACHKUB_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO6653507.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPAALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3425..4405
                     /gene="ydcK"
                     /locus_tag="ACHKUB_000434"
     CDS             3425..4405
                     /gene="ydcK"
                     /locus_tag="ACHKUB_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO6653508.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4400..4936)
                     /gene="rimL"
                     /locus_tag="ACHKUB_000435"
     CDS             complement(4400..4936)
                     /gene="rimL"
                     /locus_tag="ACHKUB_000435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO6653509.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4999..5223)
                     /gene="ydcH"
                     /locus_tag="ACHKUB_000436"
     CDS             complement(4999..5223)
                     /gene="ydcH"
                     /locus_tag="ACHKUB_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO6653510.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5363..7018)
                     /gene="opgD"
                     /locus_tag="ACHKUB_000437"
                     /gene_synonym="mdoD"
     CDS             complement(5363..7018)
                     /gene="opgD"
                     /locus_tag="ACHKUB_000437"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO6653511.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7243..8586)
                     /gene="ydcJ"
                     /locus_tag="ACHKUB_000438"
     CDS             complement(7243..8586)
                     /gene="ydcJ"
                     /locus_tag="ACHKUB_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6653512.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFHTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8803..9726
                     /gene="ydcI"
                     /locus_tag="ACHKUB_000439"
     CDS             8803..9726
                     /gene="ydcI"
                     /locus_tag="ACHKUB_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6653513.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAKLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9764..11404)
                     /gene="trg"
                     /locus_tag="ACHKUB_000440"
     CDS             complement(9764..11404)
                     /gene="trg"
                     /locus_tag="ACHKUB_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO6653514.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVIRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            11803..11952
                     /gene="hokB"
                     /locus_tag="ACHKUB_000441"
     CDS             11803..11952
                     /gene="hokB"
                     /locus_tag="ACHKUB_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO6653515.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(11930..11998)
                     /gene="yncP"
                     /locus_tag="ACHKUB_000442"
     CDS             complement(11930..11998)
                     /gene="yncP"
                     /locus_tag="ACHKUB_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO6653516.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(12024..12197)
                     /locus_tag="ACHKUB_000443"
     CDS             complement(12024..12197)
                     /locus_tag="ACHKUB_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653517.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12442..12972)
                     /gene="cybB"
                     /locus_tag="ACHKUB_000444"
     CDS             complement(12442..12972)
                     /gene="cybB"
                     /locus_tag="ACHKUB_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO6653518.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDWPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13161..14162
                     /gene="gap"
                     /locus_tag="ACHKUB_000445"
     CDS             13161..14162
                     /gene="gap"
                     /locus_tag="ACHKUB_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6653519.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYSDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14204..15643)
                     /gene="aldA"
                     /locus_tag="ACHKUB_000446"
     CDS             complement(14204..15643)
                     /gene="aldA"
                     /locus_tag="ACHKUB_000446"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO6653520.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15840..16640)
                     /gene="ydcF"
                     /locus_tag="ACHKUB_000447"
     CDS             complement(15840..16640)
                     /gene="ydcF"
                     /locus_tag="ACHKUB_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO6653521.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(16912..20814)
                     /gene="hrpA"
                     /locus_tag="ACHKUB_000448"
     CDS             complement(16912..20814)
                     /gene="hrpA"
                     /locus_tag="ACHKUB_000448"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO6653522.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTALGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEENGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            21015..21620
                     /gene="azoR"
                     /locus_tag="ACHKUB_000449"
     CDS             21015..21620
                     /gene="azoR"
                     /locus_tag="ACHKUB_000449"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO6653523.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLITGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(21671..22987)
                     /gene="ynbD"
                     /locus_tag="ACHKUB_000450"
     CDS             complement(21671..22987)
                     /gene="ynbD"
                     /locus_tag="ACHKUB_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO6653524.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVNEAISYIRARRPQIVLTDEHKAMLKLWENR"
     gene            complement(22977..24734)
                     /gene="ynbC"
                     /locus_tag="ACHKUB_000451"
     CDS             complement(22977..24734)
                     /gene="ynbC"
                     /locus_tag="ACHKUB_000451"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO6653525.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLNALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24750..25646)
                     /gene="ynbB"
                     /locus_tag="ACHKUB_000452"
                     /pseudo
     CDS             complement(24750..25646)
                     /gene="ynbB"
                     /locus_tag="ACHKUB_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(25646..26251)
                     /gene="ynbA"
                     /locus_tag="ACHKUB_000453"
     CDS             complement(25646..26251)
                     /gene="ynbA"
                     /locus_tag="ACHKUB_000453"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6653526.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECDQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNALLVILMLFCTILTEFCGLLAQTINGIRSFAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLIWTAINRCRSVLLMSAER"
     gene            complement(26422..28728)
                     /gene="ydbD"
                     /locus_tag="ACHKUB_000454"
     CDS             complement(26422..28728)
                     /gene="ydbD"
                     /locus_tag="ACHKUB_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO6653527.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVNIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALTHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28792..29652)
                     /gene="pdxI"
                     /locus_tag="ACHKUB_000455"
     CDS             complement(28792..29652)
                     /gene="pdxI"
                     /locus_tag="ACHKUB_000455"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO6653528.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATLMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(29883..30473)
                     /gene="paaY"
                     /locus_tag="ACHKUB_000456"
     CDS             complement(29883..30473)
                     /gene="paaY"
                     /locus_tag="ACHKUB_000456"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO6653529.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(30455..31405)
                     /gene="paaX"
                     /locus_tag="ACHKUB_000457"
     CDS             complement(30455..31405)
                     /gene="paaX"
                     /locus_tag="ACHKUB_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO6653530.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(31506..32819)
                     /gene="paaK"
                     /locus_tag="ACHKUB_000458"
     CDS             complement(31506..32819)
                     /gene="paaK"
                     /locus_tag="ACHKUB_000458"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO6653531.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(32846..34051)
                     /gene="paaJ"
                     /locus_tag="ACHKUB_000459"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(32846..34051)
                     /gene="paaJ"
                     /locus_tag="ACHKUB_000459"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO6653532.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(34051..34473)
                     /gene="paaI"
                     /locus_tag="ACHKUB_000460"
     CDS             complement(34051..34473)
                     /gene="paaI"
                     /locus_tag="ACHKUB_000460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO6653533.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(34463..35890)
                     /gene="paaH"
                     /locus_tag="ACHKUB_000461"
     CDS             complement(34463..35890)
                     /gene="paaH"
                     /locus_tag="ACHKUB_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO6653534.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(35892..36680)
                     /gene="paaG"
                     /locus_tag="ACHKUB_000462"
     CDS             complement(35892..36680)
                     /gene="paaG"
                     /locus_tag="ACHKUB_000462"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO6653535.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(36680..37447)
                     /gene="paaF"
                     /locus_tag="ACHKUB_000463"
     CDS             complement(36680..37447)
                     /gene="paaF"
                     /locus_tag="ACHKUB_000463"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO6653536.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(37444..38514)
                     /gene="paaE"
                     /locus_tag="ACHKUB_000464"
     CDS             complement(37444..38514)
                     /gene="paaE"
                     /locus_tag="ACHKUB_000464"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO6653537.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(38522..39019)
                     /gene="paaD"
                     /locus_tag="ACHKUB_000465"
     CDS             complement(38522..39019)
                     /gene="paaD"
                     /locus_tag="ACHKUB_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO6653538.1"
                     /translation="MQRLATIAPPQVHEIWELLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(39034..39780)
                     /gene="paaC"
                     /locus_tag="ACHKUB_000466"
     CDS             complement(39034..39780)
                     /gene="paaC"
                     /locus_tag="ACHKUB_000466"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO6653539.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHMALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(39789..40076)
                     /gene="paaB"
                     /locus_tag="ACHKUB_000467"
     CDS             complement(39789..40076)
                     /gene="paaB"
                     /locus_tag="ACHKUB_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO6653540.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(40088..41017)
                     /gene="paaA"
                     /locus_tag="ACHKUB_000468"
     CDS             complement(40088..41017)
                     /gene="paaA"
                     /locus_tag="ACHKUB_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO6653541.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTIPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            41302..43347
                     /gene="paaZ"
                     /locus_tag="ACHKUB_000469"
     CDS             41302..43347
                     /gene="paaZ"
                     /locus_tag="ACHKUB_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO6653542.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELLPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            43595..45868
                     /gene="tynA"
                     /locus_tag="ACHKUB_000470"
     CDS             43595..45868
                     /gene="tynA"
                     /locus_tag="ACHKUB_000470"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO6653543.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKSVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGIIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNLNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVIWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(45926..47425)
                     /gene="feaB"
                     /locus_tag="ACHKUB_000471"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(45926..47425)
                     /gene="feaB"
                     /locus_tag="ACHKUB_000471"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO6653544.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            47661..48566
                     /gene="feaR"
                     /locus_tag="ACHKUB_000472"
     CDS             47661..48566
                     /gene="feaR"
                     /locus_tag="ACHKUB_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO6653545.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48738..49067)
                     /gene="ydbL"
                     /locus_tag="ACHKUB_000473"
     CDS             complement(48738..49067)
                     /gene="ydbL"
                     /locus_tag="ACHKUB_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO6653546.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(49072..49257)
                     /gene="ynbE"
                     /locus_tag="ACHKUB_000474"
     CDS             complement(49072..49257)
                     /gene="ynbE"
                     /locus_tag="ACHKUB_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO6653547.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(49254..51893)
                     /gene="ydbH"
                     /locus_tag="ACHKUB_000475"
     CDS             complement(49254..51893)
                     /gene="ydbH"
                     /locus_tag="ACHKUB_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO6653548.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMEKSQMPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            52101..53090
                     /gene="ldhA"
                     /locus_tag="ACHKUB_000476"
     CDS             52101..53090
                     /gene="ldhA"
                     /locus_tag="ACHKUB_000476"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6653549.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            53201..53623
                     /gene="hslJ"
                     /locus_tag="ACHKUB_000477"
     CDS             53201..53623
                     /gene="hslJ"
                     /locus_tag="ACHKUB_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO6653550.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(53620..53886)
                     /gene="ydbJ"
                     /locus_tag="ACHKUB_000478"
     CDS             complement(53620..53886)
                     /gene="ydbJ"
                     /locus_tag="ACHKUB_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO6653551.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            54160..57684
                     /gene="nifJ"
                     /locus_tag="ACHKUB_000479"
     CDS             54160..57684
                     /gene="nifJ"
                     /locus_tag="ACHKUB_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO6653552.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRYDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        14603 a        15207 c        13778 g        14270 t
ORIGIN      
        1 gaatctttaa gccattgcgt ttcagtggca tttttccact caaccagcca ggaagccatt
       61 ttcccgtaag ggatgtattt attaccggcc tcatgattct cattctgacg tgccagagca
      121 cgattgaaaa cgaaacgaca tgcgcctgcg aagcgcctca tttcacactc ctgttgacca
      181 cctggtctta actggaattt aaatgcctgt aatcgtttca caggtcaaat gatactttgg
      241 tctgtgaata aagaaaccga tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt
      301 cacctggtct ttgtcaccag ataccgacgc cagatttttt gacgcgacag aaaaactacg
      361 cacttacttt tcaaatgtat gtgcttattt tgaagctgaa ctggttgaaa tggatggcga
      421 accagatcac gtccatttgt taattaacta acctcccaaa ctggcgatat ccagtctggt
      481 aaacagcctc aaaggcgtat cgggtaggtt actgcgacga gatcgaccag atattgcagt
      541 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcggagg
      601 tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg gtcaggtgga
      661 aaaccgcacc ttatatcccc gccctgaagg atggggtttt acggcacacc ggataaaaag
      721 cgccatctaa cgatggcgcg tctggaacta ctttttgtta acgtcaaaca tggtggcatc
      781 ggtcgccatg tcatcaataa cttgtttaat attttcgaaa gccattggcg tactttcgtt
      841 attcaggtct ttaccttcgc cctgacgaac gactttgata accggtttat tagtcgctgc
      901 atcaatcagc tcaccttcaa aatagaggcg agtatccatg gtacggtggc ctgtagccat
      961 ttgcgtcccc gcaaccacta atgcaacagg aaccacttca tagaattgca gcccttcttt
     1021 gctggtatct acaccggtaa tggccccacg gaaaatcaga ctacgcggcc cagcggtggt
     1081 aaccagtggt ttacgctgcg ctatcgcttc tttcatttcg gtgttggtat agttcaaaat
     1141 tttatccaga actttctgcc ctacctgggt agaaggtttt ggcaccggat aataagtgat
     1201 tgggttccag acgatgctgt catatttgct ttgatcaaaa ctcgggtcta cccaacgtaa
     1261 aacaggttta cctgttgctg aggttgtttc ttttaaatca gagtaattgt ttaaaaaacc
     1321 agaatattta tctggctggg tgattttaga ggcacaacca gacagagcca acaagccgca
     1381 taaagctgca actttcgcaa atgatgtggt acgcatgaat agtttcctga gatgattata
     1441 ttacttttta agttatagca aaaagcgccg cttcgtgttg tggcaaaaaa agtgttggtg
     1501 aaaataaatt tttcagcccc ttttcagacg acatagtcaa tgccgaaaaa ggggctaagt
     1561 caatcatcag tagttaaatt tcagtaagtt aagctgctaa aaaacttcga ctactgtgat
     1621 taatttcaca tcccttcgat aaaaaatatg cttaccgaaa agaagaacag taaatcatct
     1681 cagcgtgggg aattttcacg cgccttattc ttagcgggtc attttttacg tgccagcatc
     1741 gtggcgaaac gcagtttgat acgattaccg ttggcgtcgg tgcggtgcag ctcgccgacg
     1801 tcttcattgt atttcaccct ctcccagcct tcgtaatatc gacgtaattc tccctctttg
     1861 aaggcaaacg ggaagccgac ggtacatgga taatcagcgg tatccatcgc cgccacaatc
     1921 aggttgtaac caccaggttt agtgcaacgt tgcatattga caatcagccc tgggatggtt
     1981 ttagcctcaa ggaacatcag taccacagtc gaaagaataa aatcgtactg tctatcaaat
     2041 gtgaggttat tcagatcaac gactcgggtg tgtaaattat ccagattttc aatggattta
     2101 atgcgctcga cgttggcgat actcatggca tttttatccc atgcgtcaac atcataacca
     2161 ttggctgcca ggtaaagact gttacgacca ttgccacagc ccagatccag cgttttaccc
     2221 ggtttaacca ctttcaccgc ttccagtact tcagagtgtg tgcgggttaa ttcatattta
     2281 tcagtaaaat agttttcgtc acgaatgatc attctttgtc ctctgctttc attaaaacgg
     2341 cgcgctcggt tctgactaac aattttccct gcatcagaag cgcaaaagta cggatgagca
     2401 gtattgcaat aataaaattg gtaaagataa acagcggcac cgccagcgta tggaaaaatc
     2461 cattatcgct gccactcccc agatgcaaac cggtggttgc cagtgcagat acgccgaacg
     2521 agaaactcca gaatgaagca ttaaatggct gggagagata ccatggcatc agacgtagca
     2581 taaacagcag ttgcagcagt ccataaccga aaagcatttt cgccagcgtg tcaccctcgc
     2641 cgccgttgac gctcagccag gcactacaag ccaccagcgc aggagcgagc tgaatgccga
     2701 gtgatgtccg cagtgccgcg ggtaattctc ccgaactgcg cagacgctgc aagatcaccg
     2761 gttcaaggct tagccatgag aaaacgcctg cgcctaaaaa caccagaccg gcgtcggtgt
     2821 agcccaacgc accacaggcc attgcgctga taaagttgtt ggcaactgtc ggcagataca
     2881 gtccaggcgt ggtagcttct tcagggtgag atccgcgcca taatcccgca gtttgccagg
     2941 cggcataagc caactgaacc acgacaccaa aactgaacag gcacaccgcc agtgggcgaa
     3001 accacggaac aaaaccaatc gccaccagca tcgtcgttgc cggaaacaaa ctcacaaaac
     3061 tgctcagcac tggatggcga acttccgcca gcacgctatg cggaaagcgt atgagtcggg
     3121 taataaatgc gctagttaat aatccccaga tgatcatcgc cagaatcacc agcccatccc
     3181 ctaaccagtg gctgacctgc caaacctggc tggcatagcg ccaggcaaat cccatcccta
     3241 tcgtccccaa cacaatacca aagtagcctg ccggcaaatt gagcacttta tcgctctgca
     3301 tcttcttact cattttgttt aatttataaa gtatatttga aatgcatttt atggaatttt
     3361 ctcgagcata gccagagccg cagaatttgc tacggttata gtaagtcacc tgtgggaaaa
     3421 cgctatgcgc aaatatcgtc taagtgaaga acagcgagcc tttagttatc aggaagatgg
     3481 cactaaaaaa aatgtgttat tacggcagat tatcgccatt agcgatttta acgatgtgat
     3541 agcaggaacc gctggcggct ggatcgatcg cgaaacggtg ttagcacagg aaggaaattg
     3601 ctggatttac gaccagaacg ctattgcgtt cggcggagcg gtgatttccg gtaatacgcg
     3661 catcaccggc accagcgtgc tgtggggaga ggtttacgca acggataatg tctggatcga
     3721 taatagtgaa atcagccagg gtgcatatat aagtgacagc gtcaccattc atgactcact
     3781 ggtttgcggg caatgtagaa tatttggtca cgccttaatt aatcaacatt ctatgatcgt
     3841 cgcagcacaa ggcttaacgc ccgaccatca actcctgctc caaatttatg atcgcgcaag
     3901 ggttagcgcc tcgcgtattg tccaccaggc acaaatttat ggcgatgccg tcgtccggta
     3961 tgcttttatt gaacatcgcg ccgaagtttt tgattttgcc agtgttgaag gtaatgaaga
     4021 gaacaacgtc tggctatgcg attgcgctaa agtctacggt cacgcgcagg tgaaagctgg
     4081 catagaagaa gatgccattc cgacgattca ttacagttcg caggtggcgg aatatgccat
     4141 tgtggaaggt aattgtgtgt taaaacatca tgtcttgatt ggcggaaatg ccgtggtacg
     4201 tggtggaccg attctgctcg acgagcacgt tgtaatacaa ggtgagagcc gtatttcagg
     4261 tgcggtgata attgaaaacc atgtggagtt aaccgaccac gccgtcgttg aagcctttga
     4321 tggcgacacg gttcatgtcc gtggtccaaa agtcattaac ggcgaggagc gcattacgcg
     4381 aacgccgctg gcagggttat tatgaatcga taatacgcgc gtataagttc acatcatcat
     4441 aggcatcatt caggaactca gcctgtttca ggcaaccttc aaggataaaa ccattgcgca
     4501 aagcgacctg gttgctttgc ggattgtcca cccgacattt gatcacgaag cgtctaagtt
     4561 caccagactg agcgtaatga tgaatcaatg cctgcagcgc ctgagaaatg atcccctgcc
     4621 cctgatgaga ttcgtccagc cagtagccga tttcagcagt tttattcagt ggttcaatac
     4681 gattaaacga gataacgccg ataagttcat cttctttgaa aatcatgaac attttggcat
     4741 agccgcgttg atgcaacatc acattaccct gcaccgtttt tcgcgtgtcc tcttcactct
     4801 gaacgaactg cggccagttt agcgactgct gtaaccaggt tttattttta cagattaact
     4861 gataaagagg tgtgacgtga ctttccgcaa cagcatgtaa ttcaagtgat tcgcttactt
     4921 ttatcgtttc agtcatctta tctccagtca atccatgctg ttgttaaaaa agccgcctgg
     4981 ctggcggctt aggaaagttt acacctcttt gacgctctcc tgctgcagga ttttgagcat
     5041 ctcatctttt aactgtagct tttgtttttt catgcggacc acttccgcat tgtaccctcg
     5101 accgtcggaa ccttcctttc tggcaatttc atgatcaagt ttattgtgtt tatcgaacaa
     5161 ggacataaag cgaggatttt cgtttttcag acgggatatt aaatctcggt attctggaaa
     5221 catacgcact ccctgttagt ggctgacaag gtgtatctga aatacacacc atcagattac
     5281 tcatttaact tagggaaaaa tgcgagcggg atcgtgttta accagacggc acctcccgaa
     5341 gaaggtgccg aagaaaaaac gattaactca tcacgcggtc gtcaacatac tgacgtttat
     5401 ccggcgctgg cgggaaatac tgatacagcc atgtttcact gatagcgtcc ccctgacaac
     5461 gtagatacat ccgcatatcg accggatcag tggagtccga agtcggatac cagtcaaact
     5521 gaatacgata accatcgatg ggttcaatat agagaatttc gatttgcttc gcttccccac
     5581 tggaaagcgt aatcaccggc tcaatgcctt ttggcgcggc agctttcaga tcaccaccaa
     5641 cgaaatcgac ggcaaaacga cgcgcccatt tttcgggata gtgttcacct ggcgcccaac
     5701 cttccgggaa accgcccatg ccggtacgcg tcgccataac gcgcgctaat gggcaatgaa
     5761 caggcggttg cgcactccag tacagacgat actggaatgc aaactcatca cctgctttta
     5821 cagctttttc tggctgccag aagcagacaa tgttatccag cgtttcgccc gttgttggga
     5881 tttccatcag gccgatggtg cccttacccc acttgttacg cggttccacc cacagacttg
     5941 ggcgtttgtt ataccagccc ataatgtcct gataatggga gaagtcacga tccagttgca
     6001 ataaaccaaa ccctttcggg ttgttgtcgg tgtaagcatt gaactgcaat ttttgcggat
     6061 tattcagcgg acggcaaatc cactcgccgt tgccccgcca catggacaga cgatcagagt
     6121 catgaatttg cggatgaatt gtatcgcaca tccgacgttc attagtaccg cagctgaaca
     6181 tactggtcat cggcgcaatg cccagctgtt taatgtcttt gcgcgcatac aggtgatttt
     6241 ccacatccat aatcacctga cttttctcac aatggatagt gaacttatag gcaccagtaa
     6301 tgctggcgct atcgagcaac gcataaacgg taaaggtagt tgcccccggt tttaccgtat
     6361 caaaccagaa ggcggtaaag tcggggaact cttctttact gtcggtgtaa gtgtcgatcg
     6421 ccaggccgcg ggccgacaaa ccgtattgat atgtatcatc aacggcgcgg aaataactcg
     6481 cgccgagaaa tgatactaca tcacggcgcg ccagttcggg ggctttaaac acgcgaaaac
     6541 cggcaaagcc gagatcgctt tgcccttcta attgtttggt atcaacaccc gcatcgttgt
     6601 atttgaacaa ctccgggcga aagtgaattt cacgcgccag atgtgttgct ggatctacag
     6661 aaaacatacg aacgcggcga cggaatccca ttcccatatg gaagaactga gcgtccagtt
     6721 gacggttctc aacgttatgc cagagcgatt tttcggcgtc gtattgaata ctgttataag
     6781 cctgcggcgt cattgtcgcc agcgtgtcag gtaacggacg aggcgcacca cgccacgctg
     6841 tttgcgctaa gtcgtgcgcc attgactgta gaatggagaa gtcaaaacgc tgggtttgcc
     6901 cgtcggcaat atcagaatct gccgcgaatg ccgcctgaga aaaaagagaa gcaatgccgc
     6961 tggtaccgca cacggcggcc atagccattg aacctttaat aaatcgtcta cgatccatac
     7021 ctgaaagtga gtccttctgg tgttgtgaat ggtcccgcgc tggtcgtttt cagcggcaag
     7081 aaagtgctta tttatgaccg ctcactctag acaatattta ttaataatcc aattgttgaa
     7141 ctgagagaat atgcataaat cggcattttt tttatgtcga tgggatgagc ctgattcgtt
     7201 tgtaaaatca ggcaaatttt tgcaaaggcg gatggtcaga atttaaagca aaccacagcg
     7261 acgtttactg cgttcttccg cttcctggta aagctggaac tcatccaaaa ccggacaacc
     7321 caacgcctgc tcaaatgctt cgcgactggc attaccgtga ctgcgtgtct gcgtttcatt
     7381 acccagattt gactggaaga tccccgccgc gctaacgggc aagaaatctt cataggtgat
     7441 gggttgcgcc actacccaac cacgttcaat taagggctgt ggatcgtctc caggatgaat
     7501 cgcctgacga tgcgcctcac ccgaaggcgt cagacggtac cggaaccatg ccaacccttg
     7561 ctgacgcatt aaaaactcac tgtcaggaaa agtgtggaag gtttcctgta aatgcatttg
     7621 gtgagtgaga ttatcctgcc cggttccagc gttacgcaga agatcatcat acagttgtcg
     7681 ccctttcggc gttaatgcca cgccacgctg ctcaatttca ccaaagcgcg cggtatgcgt
     7741 gccctgtttc tgccccgcaa acaacaccgt ctcttccagt gctttaaagc tggtctggcg
     7801 tagtaaaatc ggtacctcgc ggcgcggcgg cccctcgatg agaattttgg gttcaattcc
     7861 gcattcaggc atcatcgact gcacccggtc aatatccagc gtacgtggcg tcaggtggtt
     7921 gatatggcat ccaggaaaac agaccacatc agcaatcaac cgatgttcgt tgtgcaaggc
     7981 gcgataggtt tcttcatcta ccgttgctga ctggtgccag cgaaacgttt ccagggcttc
     8041 ctgcacaaac tcctgtgcct gtgtttcgtt aaaaccgccc tgctgctcat attcctctaa
     8101 cagttgtcga caacgtgggg tgaagatatc gcgctgacgc agaatctccg ccgctttctg
     8161 gcgcaaaatt tcgttctcga taagctcaag gcggagtaag gaggtaaaaa cgcggaaggg
     8221 attacgcgcc agagaagcat catcaatggg ccgaaatgct gtcgaatgta ccggcacccc
     8281 tgcctgcgag agatcgtaat agctcaccgg gtacatcccc ataatggcaa acatccgccg
     8341 aagagtagca agctcttgtg cagtcccaac gcgaatcgcc ccatgacgtt caacattcag
     8401 tcgcgccagc tcgtctgcat ttaccatttt ttcgtgcagt tgaggattgt tttccagcac
     8461 agccagattc acatcagcta ccagttccag cagcgtgcca tattgcggaa cttcttgctg
     8521 gtacatggct gacattgcct gcgaaaactg ttcccgaatc tcatccgccg tgatgctgtt
     8581 cgccatagtg tctgcctcca gtggtcagta atctggagtg taggtaaccg cattcactct
     8641 tgcgggaaga atttacaaac tgtgatctcg ccgcgaaaac atcaatatta tccattttgc
     8701 tgtaacataa ttgctttaat tgttaataat attttgcaat caagttatca taatcaaaca
     8761 acttcacttg tcagcgacac cgcttcgttt ttaacatcgc ttatggaaaa aaatagtctg
     8821 tttagtcagc gcatccgttt gcgccacctt catacattcg tagctgtcgc acaacaagga
     8881 actttggggc gcgcggctga aacccttaat ttgagtcaac ctgcgctctc taagacattg
     8941 aatgaactgg agcagctgac tggcgctcgc ttgtttgagc gtggtcgtca gggggcgaaa
     9001 cttaccttac ccggcgaaca atttttaacg catgcagtca gagttcttga cgccatcaac
     9061 actgccggac agtcgcttca tcgtaaagaa ggtcttaata atgatgtcgt cagggttggt
     9121 gcactaccta ctgcggcact ggggatatta ccttcggtta taggtcagtt tcatcagcaa
     9181 caaaaagaga cgaccttgca agttgcgaca atgagtaacc ctatgattct ggcgggttta
     9241 aaaaccgggg aaatcgatat cggcattggt cggatgtcag atcctgaact gatgaccggg
     9301 cttaattacg aactgctgtt tcttgaatcg ttgaagctgg ttgtccgccc taatcacccg
     9361 ctacttcagg agaacgtaac gctaagccgg gtgctggaat ggccggtcgt tgtatcacca
     9421 gaaggcactg cgccacgcca gcattcagat gcattagtgc agagccaggg ctgtaaaatt
     9481 ccttcgggtt gtatcgaaac gctgtctgct tcgctatctc gtcaacttac ggttgaatac
     9541 gattacgtgt ggtttgtccc ttctggcgct gtaaaagacg acctgcgtca tgccacgctg
     9601 gtggccctgc ctgttccggg acatggtgca ggcgaaccga ttggaatact gacccgcgta
     9661 gatgcgacgt tctcttctgg ttgccagttg atgattaacg ctattcgaaa atcaatgccg
     9721 ttctgaaagg tgaagggatc tgtcgatccc tccttgaaca ttttcacacc gtagcgaaac
     9781 taactggttc acccgctccg cgaggttctg ccgacacaga atgtttgtgc agacggaata
     9841 catccaccgc ctcagttaat cgtgccgcct gttcttcaag ggacaccgcc gccgctgagg
     9901 cctcttctac cagagaagca ttctgttgcg tcaccttatc catttcagaa atcgcctggc
     9961 taacctgcgt tatgcctcta ctttgttcat ccgaggcggc ggcgatttcc tgcatgatat
    10021 gtgtgacact cgcgacggca tcaacaatag tgctcatggt tttccctgcc gttgccacct
    10081 catccgaccc caggtcaatt aacctgactg attcactgat caagccttca atctctttcg
    10141 ccgcttgggc gctgcggctt gcgagtgtcc gtacttcgct ggcgacaacg gcaaatccac
    10201 ggccttgctc acccgctcgc gcggcttcaa cggcagcatt cagtgccaga atattcgtct
    10261 ggaaagcaat actgttgatg acggcggtga tctcggaaat tttcttcgaa cttgtagaga
    10321 tagcgcccat cgtttttact acaccggaaa ccgtctgccc gccatcgctg gctttaatag
    10381 aagcctcttg cgccagtttg ctggcatgat gcgcgttatc cgcattctgt ttcaccgtcg
    10441 cagtgagttg ctccatgcta gcggcagttt gttcgatagc cgccgcttgt tcttcggtgc
    10501 gagatgacag gtccgcattg ccagctgaaa tttcgctggt gccacgataa atctcttccg
    10561 caccctgtcg aacagtccct actgtcatcc ccagtgaatg ctgcatttgc tgtaaatgac
    10621 gatttaagcg accgatttca ttacgacccg ccggttcatc gttcatcgtc agatcgccac
    10681 tagcgatttt ttcaatccgt tgtgcggcat gttgcagtgg acgaatgacg atccgacgta
    10741 tcaccataaa tgttatcagc gtcatgacca gggcaagcac aaacgcgcca atcatgaaca
    10801 tcccacccag gcgggtgcgc tgatgggcca gttccgccag ttggttggct ctggtgctac
    10861 gtatcttaac ggctttgttc aaaatatcgg tataagcatt atccagcggt cggatctgct
    10921 cactttcatg attaataatc gcttcaaaca tgccattttt ggcatatttc aacataggtt
    10981 gcatacccgt gatataagcc tgaaagcgtt gatttaattc agtgtcgagg gcttcatcag
    11041 caggtgtttt caccggccga ttctgataag cacgataacc ttgctgcgac tgtttaatct
    11101 ccgattcggc ttgcgcaata tttcgcttca ttgcttccat ttccgcaata cgactcgcag
    11161 ccccggcctg aatcatattg atccgggctg aacgcaaaaa atctgaactg ttcgctaacc
    11221 cggtccgaat ctcaatctcc gcagtaacat cacgctgatc gcgatcggcc tgccagagga
    11281 aatagccagc cagagcagaa cttaatgcga atagaactaa gataccgcca agaatgcagg
    11341 caaataacgg aaccagtctg atgtgatgca aaaaacctaa tcgctgtgag ggagttgtat
    11401 tcattaccgt tgtctctcgt ccaggtttac tcctgataga aagtcatcgg cacttgacgg
    11461 taattactta tgaccaaaaa cctctttgcg tcacattttt acaacaatta ttgcagagaa
    11521 aattaatgct attggaatga agagagtaac gaatggctaa actggctaac ccgaatgacg
    11581 aaaaattctg gaaaacagca cgcaatttcg tgaagtgatt attttcgtcc ctaaggaaac
    11641 ctcataggat gcctgcgggt gcctgagact ttctgttttc aggctaaccg ggaatcagaa
    11701 agacgaaggc cccgaccgat atttctatca accgaggcca acgaatgaac ctagcaattc
    11761 agatcttagc ctcttacccg ccatccggca aggagaaagg ctatgaagca caaccctctg
    11821 gtggtgtgtc tgctcattat ctgcattacg attctgacat tcacactcct gacccgacaa
    11881 acgctctacg aactgcggtt ccgggacggt gataaggagg ttgctgcgct catggcctgc
    11941 acgtccaggt aagggcaagc gcggggattt tccccgcgca tctgactagc agattcattt
    12001 tctcatggca ccccttttac acttcagtta gtgcatgttt ttttcgaggc actaatagaa
    12061 ccatcattac agacaaattt gccgcctgcc gtacagtgtg agatcccccc tttcgaacca
    12121 gaacagggtt tgcgcccggc atcggcataa aaggaaacaa gcgttcccat aaacaaaata
    12181 agtgccagtt ttttcatata acctccatgg tttatcgaat cttatgttcg atttgaacgt
    12241 tatacgctgg tttattttga taccgttgat gagatcaaag atcgggtgaa ttgattaaaa
    12301 ataagccaga tggacgtatg ggcaaggatt atggttttat ttgtcataca aataaatata
    12361 ataggcgctt ccgatgtaga cccgtattct tcgcctgtac cacgggtcgg ttttagtaca
    12421 ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc atcatgcgta gaagtgtgtt
    12481 gtccttccag aaatagtggt gtgccagtgc agccgcagcg tgcaacccga tgacaaaata
    12541 tcccagattc gccagcgtct catgccacga ctttaagcta tcaacccgtt cgaaattggc
    12601 ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac gggttgcccc ggttatacat
    12661 catcaccaaa ccaatcactg gcagcgcaat aaacaggaga taaatcacca aatgtcccaa
    12721 atgcgccagt cccgtcatca tcggttttgg cttaggtata atcggcgggg ttgggtattt
    12781 cagtcttaac aaaagacgaa ccaccatcag cacgagaatt gagataccac aggaaacatg
    12841 aaccatgttg atgagtggcc aatcgctacg tgggaagaaa ccacgaaact ccattgcgca
    12901 atacgctgcg ataaccagca aaaagaccag ccagtgaatg ctgatttgta accttgagta
    12961 tttattttcc ataacatttc ctgctttaac ataattttcc gttaacataa cgggcttttc
    13021 tcaaaatttc attaaatatt gttcacccgt tttcagtacg ttacgtttca ctgtaagaat
    13081 ggagattgcc catcccacaa tcctggtcta agcctggaaa ggatcaattt tcatccgaat
    13141 gttcctgaca ggagaaaacc atgagtaaag ttggtattaa cggttttggt cgtatcggtc
    13201 gactggtgtt gcgtcgatta cttgaagtca aaagcaacat agacgttgtc gctattaatg
    13261 atctcacttc cccaaaaatt ctcgcctacc tgctgaaaca tgattcaaac tacggaccat
    13321 tcccctggag cgttgatttt acggaagatt cacttatcgt tgatgggaaa agtatcgcgg
    13381 tttacgccga aaaagaggct aaaaatattc cgtggaaagc gaaaggtgca gaaatcattg
    13441 tcgaatgtac tggcttttat acctccgccg agaaatcgca ggcgcatctt gatgctggtg
    13501 cgaagaaggt gttgatttcc gcccctgccg gtgaaatgaa aactatcgtt tataacgtca
    13561 atgacgacac tctggatggc aacgacacca ttgtttccgt ggcgtcatgc accactaact
    13621 gtcttgcgcc gatggccaaa gccttgcatg acagtttcgg gatagaagtc ggcacgatga
    13681 cgaccattca tgcctatact ggcacccagt cactggtgga tggcccacgt ggtaaagatt
    13741 tacgtgcttc acgcgcagcg gcagaaaata tcattcccca cactacgggg gcggcaaaag
    13801 ccattggtct ggtgatcccg gaactgagcg gcaaactgaa aggtcatgcg caacgcgtgc
    13861 cggtgaaaac aggttcggtc actgagctgg tgtccattct cggaaaaaaa gtgactgccg
    13921 aagaggtgaa taacgcactt aaacaagcaa ccaccaataa cgagtcattt ggttattccg
    13981 atgaagaaat agtctcttcc gatatcattg gcagccattt cggttcggtg tttgatgcca
    14041 cgcaaacgga aattaccgcc gtgggcgatt tacaactggt gaaaacggtc gcctggtacg
    14101 ataacgaata tggcttcgtc acgcagctta ttcgcaccct cgaaaaattc gctaaactct
    14161 gacgcgcaca ggcggaggaa aaaacctccg cctcttttac tcattacgac tgtaaataaa
    14221 ccacctgggt ctgcagatat tcatgcaggc catgtttacc atctgcgccg ccaataccgg
    14281 atttacgcca tccggcgtgg aagccttgca tagcttcgaa gttttcacgg ttgatgtaag
    14341 tttcaccaaa cttcagccct ttaatggctt tcatcgcgac gttcagattt tgggtataga
    14401 ttgatgaggt caggccgtaa tcactgtcat tagccattga gatagcctct tccagcgtgt
    14461 caaatgcgac caccggcaga accgggccaa aggtttcctc atgcataatc gacatttcct
    14521 ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccctct accgctttgc
    14581 cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc tcaacccttt
    14641 ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttca gcggggttac
    14701 caaattgaac cgcctgcatc gcttcaccca gccgattgac gaactgatca taaatgcctt
    14761 tctgtacata aacacgttct gcacagttac acacttgccc actattaatg acgcgtgaat
    14821 caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata gctggtgctt
    14881 taccccccaa ttccagacac actttggtga tgtttttcgc cgcagtcgcc atgatcttct
    14941 cacctgcaga gacgctgcct gtcatactga ccattgcgac ctttgggtta cccgccagtt
    15001 cttgcccaac ggtttcacca cgccccagta caaggttaaa cacgccgcgc ggaaggccta
    15061 tttcatcgac gattttggcg aatgcaatcg cattgtttgg cgtaaattca ctaggtttaa
    15121 tgacgatggt attaccggtc aaaagagcgg gagccatttt gcgggcaatg aggaagaacg
    15181 ggaagttcca cggcagaatg ccggtagtca caccaagcgc acgtttaaac aaaagaatat
    15241 tttctcctgg acgatcgctt tgaataatct cgccctcgta acgccgtgcc cactccgcca
    15301 tgtaatcgat atagtcggca gtaaaagcca cttcgacttc agccagctgc tggatcttgc
    15361 ccccttcttc aacaatcagc gcactgattt cactggcgcg ttcgcggatc ccggcggaga
    15421 ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc ttcccattct ggttgtgcac
    15481 gttctgctgc atcgattgcc ttacgggcat cctcggcctg accatcgggt atgcgggaaa
    15541 tgacagcctc tgtagcaggg tttaccacat caatccatgc gtctccacgc caggtaacaa
    15601 actgtccatc gatatacata ggatgttgaa cgggtactga catgggcgac tcctgtgatt
    15661 tatatgttta ttttcggtga atgcattgtt aattaacatc aatcatcaga gagacggcat
    15721 cgtgaaagga caattctgtg aagggcttca taaaaacaag tgtaaaagga ataaaaggca
    15781 gaaataaaag tcaggagtca tgcccgtttg caaacaagtg gttcacaaac gggcaatttt
    15841 taacgtaacg agcgactttc catcgcctcg atgagcaccg catcatgttt cagcgtttgc
    15901 catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg cccgcgggga
    15961 ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag tgagagataa
    16021 cgctcaaccg gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcattattt
    16081 cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtcccca
    16141 gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg aacaacgata
    16201 gccgtatgaa ctcgttctac ggcctgtttc aatagcgcga tgctaaagcg tgcgttttca
    16261 ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatgtg ccagaactga
    16321 tgagcgatat ccgccaggat ggtcgcttct gctctgccag tggtgcggat agtgttgtag
    16381 tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat accaccacta
    16441 atcagtaaag gaatttgctg atcgcgagca atcttacatg ccgcgtcgat agtcggcata
    16501 accgcattgc ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc ggagaaatca
    16561 tcctgcgcca gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc
    16621 ggaaacgggg taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat
    16681 aatagtgaga agcggagcac tctgaaagga ggaagtcgta tgtccttaac cgccactccg
    16741 caagcctgac atatctttta aggaaatgct gtagataaac attattcatc ttgcagcaga
    16801 gaattgtatt tttctgaatt cctgaatttt tacccttacc gttttagaat taataaaaaa
    16861 cccggcgcta aatgcttacc gggttttttc tttatcaggc aaatagcagg gttaaccgct
    16921 aatctgctcc atcgcctgca aaatacgctt atctgaaatc ggataaggcg taccaagttg
    16981 ttgagcgaag taactaacgc gcaactcttc tatcatccaa cggatctctt tcacgtcttc
    17041 atcctcacga cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct gctggacgtt
    17101 ttcgactttc agcatctgag cacggtcgcg atgtggatca accgccagtt tttccagcct
    17161 tttttcaatc gcctgcaaat aacgcagcgt gtcgcccagc cgtttgaagc cgttaccagt
    17221 gacaaaaccg cgatatacca acccgcccat ctgcgcttta atgtcagaaa gccccagcgc
    17281 catggtcata tccacccgcc ctttcagacg tttgttaata ttgaacaccg cagtgaggat
    17341 ttgctcgacc tgcttcgcaa tatccaccac cgtgtcgttc agttcagcgc ggactttttc
    17401 atgcagcgca gcaaagcctt cttccgtcca gaccgggcca ccattggcgt cgatcagttg
    17461 atccacaccg caggagatac agtcgtcgat cagctccagc actttgccat acgggttaaa
    17521 gtacagaccc agcttggctt tgttcggtaa cttttcatgt aaatacttga ttggcgaggg
    17581 aatattcagt agcagtagac ggcgaagacc gttccacatt gcctgctttt gttccagcgg
    17641 attatcaaac agtttgatcg ccacgctgtc gcgttcatcc accagcgccg gccacgcctt
    17701 cactttgtag ttgccacgct tctgttcgta gctttccggc agctgaccaa aactccagat
    17761 atgtaagccg ctctgctcga taccgtcatc cgccaccgca gacagcgttt cctgcacttt
    17821 gcctttcagc gcatctttca gatcttgtag cgagcgccct tcttttagct tcttgttttt
    17881 gtcatccacc acgcggaagg tgattttcag gtgatcgggc acctgatccc agtgccagtc
    17941 ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc tgtcaagcaa
    18001 cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg gcgcgggtac
    18061 aaaattacgg cgtaccggtt tcggcaacga tttaatcaga gcaatcacca gttcgcggcg
    18121 cagaccgggg atctgccatt caaacccatt ctcctcaacc tggttaagta acggcagcgg
    18181 gatatgcaca gtcacaccgt cagcatccgc gccgggctca aactgatagc tcaaacgcag
    18241 cttgagattg ccctgatgcc agaagttcgg gtaatccagc ttgctgattt tttccgcccc
    18301 ttctttgatc aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc ggctgacttt
    18361 tttccaccag ctatcgaagt ggcgcgcaga gatcacatcg tggctgatgc gctggtcgta
    18421 gaactcaaac aacgtttcgt catcaaccag aatatcgcgg cgacgtgatt tgtgttccag
    18481 ctcttccact tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca
    18541 gtcaccttcc accagcgcat ggcgaataaa gagttcacgg cataacgccg gatcgatctg
    18601 gctgtagttg accttacgcg cggcaacaat cggcaacccg taaacggtga ctttttccgt
    18661 tgccatcacc gcgccctgcg cccgttccca gtgcggttcg ctgtaggtgc gtttaatcaa
    18721 atgctgagcg acaggctcca cccattccgg gtcgatacgc gcagcaatgc gcccccacag
    18781 gcggctggtt tctaccagtt ccgccaccat tacccatttc ggcggttttt tgaataaacc
    18841 agaaccgggg aagatggaga aacgcgcgtt acgtgcgccg gtatattctt gtttatcggc
    18901 atctttcatg ccgatatggg aaagcaaacc cgtcagcaac gcaatgtgaa tttcgcgata
    18961 ttccgccggt tcgctgttga ccggaatgcc aagttctttc accacctggc gcaactgggt
    19021 gtagatatcc tgccattcgc gcacgcgcag atagttgaga taatcggtac gacacagtcg
    19081 acggaaggcg ttagaagaaa gcgccttttg ctgctcgcca agataattcc acagatttac
    19141 aaacgcgagg aaatcggatt ctttgtcgtg gaagcgacga tgtttttcgt ccgatgcctg
    19201 ctgtttatcc atcggacgct cgcgcggatc ctgaatggag agcgcggacg tgataatcat
    19261 cgcctcacgc acgcagccat gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc
    19321 gacaggcaac tgagagagct ggcgaccgag cgccgtcagt ttataggcgc tggcctgttc
    19381 atccgtggtg atcgcgccca gttcttcgag cagacgcacg ccatcctgga tattgcgttt
    19441 atccggtgct tcgacaaacg ggaacgcagc gatatcgccc agccccagcg cggtcatctg
    19501 caaaataacc gaggccaggt tggtacgcag aatctccgga tcggtaaact ccgggcgcga
    19561 gaggaaatcg tcttcggaat agagacgaat acagatccct tcggacacac gaccacagcg
    19621 gcctttacgc tgattggcag acgcctgcga aatgggttca atcggcaaac gctgcacttt
    19681 ggtgcgatag ctgtagcggc tgatacgcgc tgtaccgggg tcgatgacgt atttaatccc
    19741 cggtacggtc agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc gtccgctgtg
    19801 cgactggaac acgcggttct gttcgctgtt cgaaagccgc gcataaagcg gcaagatttc
    19861 ggtatggcgt aagttcagct tgttcagcgc atcggcggta tcgcgaattt cccgctcgcc
    19921 gctcataaag atcagaatgt cgccggggct ttcctggctt agttcgtcta cggcgtcaaa
    19981 aatcgcctgc aactgatcgc gctcggtgtc atcggcttct tcaacaatcg ggcgatagcg
    20041 cacttccacc ggataggtcc taccggagac ttcaataatc ggcgcattat taaagtggcg
    20101 cgaaaagcgt tccgggtcga tagtcgcgga agtgataatg atttttaggt caggacgccg
    20161 cggcagcaac tctttcaaat agccgagcag aaaatcgata ttcaggctgc gttcgtgcgc
    20221 ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg tcttgctgga tctccgccag
    20281 caggataccg tcggtcatca gcttgaccat cgtgttatca cttacgtgat cgctgaaacg
    20341 cactttgtaa ccgatgcaac cgcccggctc cgttttcagc tcttctgcaa tacggttcgc
    20401 caccgttctt gccgccaggc gacgcggctg ggtatggccg atcagtcctt taatcccgcg
    20461 ccccagctcc atacagattt tcggcagctg agtcgtttta ccagaacccg tttccccggc
    20521 gacgatcact acctggtgat cacgaattgc ttcgagaatg tcctgctttt tctgactaac
    20581 cggtaaattg tcaggataag taatttccgg tcgtgccgct tcacgcagca ggactttccc
    20641 tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg catcaggatt
    20701 tttaaccttc ttcacaccgt gcagacggcg agaaaaacgc agtctgtcgc gcagcatcag
    20761 cgaatccagc cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg tcataacgtt
    20821 agtgggcagt agtctgcctg attaattttc ttttaaatga tagatataga gtaccacatc
    20881 gcagctttct atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct
    20941 ctgtggcagg attgtgaata aagtgtcagc aagcaacggg gcatcgccca ttcaaacatc
    21001 tataaggaaa caccatgagc aaggtattag ttcttaaatc cagcatcctg gcagggtact
    21061 ctcagtctaa tcaattgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg
    21121 atgaaatcac cgttcgcgac ctggctgcaa atccgattcc ggtactggat ggcgaactgg
    21181 ttggcgctct gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa gctctggcgc
    21241 tttccgatga gctgattgcc gagctgaaag cccacgacgt tatcgttatt gcggcaccga
    21301 tgtataactt caacatctca actcagttga aaaactattt tgacctagtt gcacgcgcag
    21361 gcgttacttt tcgctatacc gagaacggtc cggaaggtct gataacgggt aaaaaagcca
    21421 tcgttattac cagccgcggt gggatccaca aagatggacc aacggacctg gtgacgccgt
    21481 atctgtccac gttcctcggc tttatcggca ttaccgatgt gaaatttgtc ttcgccgaag
    21541 ggatcgcata cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa gcggccatcg
    21601 acagcattgt tgctgcataa ccggacacga tcttgatgtg gactgtagtt cagtccacat
    21661 cccaatccac ttatctgttt tcccataatt tcagcatcgc tttgtgctcg tctgtcagca
    21721 caatctgcgg gcgtcttgct cgaatatagc taatcgcttc attaacggtt ttacagtgtc
    21781 cgtaacataa caaccatgcc gccaccacca gcgcactgcg cgataatccc aatgcacaat
    21841 ggaccagaac gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga
    21901 gctccccctc ttccggaacc accagatcca gcatcggtac acaaaaatag agtcgatctt
    21961 ttgtggctcg tccgcgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa
    22021 tatgtcgtgg aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc
    22081 gacgacaaaa ccaacgcata gatagccaca tcccaatgcg ccagggcaat gtcagccagt
    22141 aaacggcggg cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac
    22201 cgtacccccg gccaataatg agtagcgata atactggcca acataaccag actgaccacc
    22261 ataactgaac catcatcatt agctccatca acacaatgca cgagcccgcg cctacgacat
    22321 atggcagtgc tattttgatt cgacgttgat caggtttctg ataattccaa cgacggtcga
    22381 cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat
    22441 gatgctgcca tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccgcagactt
    22501 tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa
    22561 tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc
    22621 atcccgtcac cccactcact tcaggacgga taaaactaaa cttcagcgga tagagcaaaa
    22681 aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg cgctgttcga
    22741 atgtcgtgct acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa
    22801 caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctggggatat
    22861 catggctgtt aaggtcctga accgcggtga actgattaag agatccatag gtgaagaaga
    22921 aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc cgacgttcag
    22981 ttatcacgac gcaccgccat cgaaaccgta aaaatacccc actcatcaat ccgttgtgtg
    23041 catttatcaa atccggcatc acgcacgagt gaatccatct ccccttgcga acgcacgcgc
    23101 attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcat ctccagttga
    23161 gggtgccacg gctgcccggt gtagatgaga atgccgcccg gttcgatggc attggcaaga
    23221 cctgcgagtg agttttttac ctgctcattt tcgggaaaca gctcatacag tccagagaca
    23281 atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc
    23341 tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatcgcttg cccctgtgca
    23401 acatttaact cactgtaatc acgtaacaaa atatcgctta cggcaggctc gtttgccagc
    23461 gcattcagta catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct
    23521 ttggcgtgga gatcggcaac ggcttgttta atcagtattt gtaaatgggt tttacgctgg
    23581 cgaataccgc gccagccaac actgttcagg tagattttgt caattaagcg cccgaatgcg
    23641 ttactgcctt gcggttgatt ttgatagaca taatccaggg aactgcctga atcaaagccg
    23701 gtgctcattc cgagatgcag gcccgcagag tgcgccccga acaatttcat cgcctttcgc
    23761 ataaagcgat acgccaaatc aaccggcgat aatggcacgg gtccacctga aaggagccgc
    23821 cagcgatccg ctgatggtcc agtgcggtct tcatgctgat aatcaaattt ttgtgacttg
    23881 ttagcatata aacgactaat aaagctttgc attttttcaa atgcctgcgc cctgttctct
    23941 tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt
    24001 aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc gcctgatatc
    24061 agaagttgcg tggggagcgt aatcgccgcc gcatctctaa taatacgttc agacgttttg
    24121 tagagatcga gcaagatatt aacggcaatc gcccgtgtga tcagcggatc attattgaaa
    24181 ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga attaataaaa
    24241 aacagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg tacatacaat
    24301 ttaaccttaa aggccggaga agccagcacc agcccgcgaa ttgcaggcgc ataatcatga
    24361 acccatgtgg caaccagcac tgcgccgacg ctttgcgcga tcactaccac ctcatccagt
    24421 ccgacctggc tatcgctggc agcaaaacga acaaattcat cgacatcctg cactgaacgc
    24481 gcaagagatg ggctataacc acgcggcccc gaagtttgtc catgccctcg ggcatcccag
    24541 gcataaaaag cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa
    24601 tgttcatggc cacgatgaaa taagacgatg acctttttcg ccccgggctg taaagcgggc
    24661 cagtgccgat aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagagatg
    24721 cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat aaaataaaaa
    24781 aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc atgtcctggt
    24841 aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat cactacatcg
    24901 ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa tgtatttagc
    24961 ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac gccccccacc
    25021 aggccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat
    25081 ttcccccata aatactgtgc aatatcgttt gactcggtta atgccaaaag aaataacacc
    25141 agtaacgccc cgccctagat attcatggat ggcaacatta ataaccaggc ggcatgactc
    25201 aaggcgaaaa cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg
    25261 gaggggtccc ccacaaaaac ttgcccggtg gctaatatta gaaacccagc caaaggtata
    25321 aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc
    25381 caatatagcc aacgcggaaa atgtatggat ataagcgtac aatattcttt caatgccaga
    25441 aaacttatca gcgcaaaaaa cgtcaacgtc atccagcgtg gacctgaaat caccagtgaa
    25501 aaacaaatga cgatacccca ccaggtacga atacgtaatg taacttcatc cccttttctc
    25561 tccggtaacc gccatagcag aaaacggttt atcagcgttg ctaaaattaa caacgcgaaa
    25621 agtgttgcca gagatttttc cagcattatc tttcagcgct cataagaagc acactccgac
    25681 aacgattaat cgcagtccag ataagcagaa ttgaagcaat gctccataaa agattattcc
    25741 attgcatcca ctgtggataa atggcaacgg caagccccca cagaccaaat attaacgcgc
    25801 gatcgctttt gccaaatgga ccggcaaaac tgcgaatacc gttaatcgtc tgcgcgagta
    25861 aaccgcaaaa ctcagtcaat atggtgcaaa ataacatgag tattacgagt aatgcgttac
    25921 tttccggtaa aaataaaaac ggtaaataga gcgcaatatc ggaaataaca tcgccagttt
    25981 cattcaaaat cgcccccaga cgtgtttgct gatcgcactc acgcgccaac atgccatcca
    26041 gtgcattgag cgccatacgg ataaaaagca cgatgggcaa tagcaaaaag aggatgggtt
    26101 gtgccaccaa cactaacagc aatccggtaa gaagagaaag cgccagtgca gcaagagtga
    26161 tgtgattcgc tgtaacgtgg tgcttataaa gccaaaacat cgtcggcctt aacagcgact
    26221 gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc ttgataattc ggtttgcgct
    26281 atggtgcatc aattcccctg acggagatat cgcaacacgc cgcttcatta aaaattcaga
    26341 atatatcaag gtattgggga ctcactgcca ggaatgatta tccccaatta atcaaatagt
    26401 taagaaacat tacgttgtgg atcaatacaa ctcatcaagt tttctcatcg cttgttcccg
    26461 tattgcgcgc gtcttaccgt taagcgccag ctggcggact aaatctagtt cagggttaac
    26521 aagctctaac gccagcaaag gatcacgttt cagcctcgcc tttaatacat caactggaaa
    26581 acggggatta ttcatcgcca caatccacca ctcgggatca atttctgccg ccagtactgc
    26641 aggcggtgtg tggcggttgt taagtgcgcc gaacttgaaa aatgtgagat tgcttttcgc
    26701 cagtgtactc caccatatac gatccggttg ttttgccagt acatccagtt gtgctaacgc
    26761 atcggggtcg agtgcataat agacgcgtaa tgcttcatca acctgcgcac gggagctgtt
    26821 ttcctgcctc agaaaaaact tcgcccaaat atcattgcgt ggcgtatttt gctgtaattc
    26881 cttgaggaca acagcgggta aattatccca actaactaat gcactctcta aaaagtccca
    26941 gtcttccaaa cgtatcaggg ctgtggcatt taatagcatc tgatcatcaa cttcaccgtg
    27001 gctgataagc tcagacaaaa tcatatcagc gctggtttca tacgcagctg aatctgcttt
    27061 cggattattt ttccgatgcg ttaaagcacg ttgccaaatt ttttgtttca tcgacgtgct
    27121 cagatactga tttttcgcca gattactcca cccctcagga tcgttgaaac gtgtcaataa
    27181 ccgttgtaga gcagcgtcag agtgaattac ctgcatatcc cgacagcaaa agtaggcagg
    27241 ggattttttc tccatccttt taacaaaatt aaactgtaag ttatctggca atgctaaaaa
    27301 taacacacgt attagatcat cgtcattctc gaatttttgg tataaatagg ctgcaacttt
    27361 gtcactctct tgtggcgtca tccgcaataa ttgaccatgg tgtttcatct ctgccagctt
    27421 gaacgctaag cttgtgacaa cattgtttga tgaatcgttc gccaactgca ttgcctgatt
    27481 gtaggtgagt tgtcctccag caccgacctg acgaatagag gaatcatcac tgcggatata
    27541 gccatcaata atggattcag gcaataacca cgcatcttta taaagctcct gaatgatcat
    27601 atcccaatct cttttcgcca gagtcatgac gatatccaga gggagatttt cacgtagcgc
    27661 aagattcaac ggaccgacac catcaggtag acgactcatt cgcgtcatta tttcaacaga
    27721 gagcggttca cggtcgagca accccagaac aacaggctgg taacccaatt gctgatcggt
    27781 caactccaga atctcattgt caggcatata tctcgccata ctttcaatcg tttgttcatc
    27841 attgctatta ataagttgtt tgcgtaggtt agctgaaatt cctttaagcc ttgggagagt
    27901 tcgcattatg gcaagatcaa atgtcgcaat atctcgtcct tcaaatgcag caacgttgcc
    27961 ttcatccagc atttgcaatt cctgcactaa ctcaggagaa ggcttagggc gaaacagcgc
    28021 ttcttttttc atctgattac agacattccc tgccatgcga atattgcttc gcttaaaaat
    28081 ttctttcaga gcatcttcat catttattag accccaataa tatgcatcag gatttattat
    28141 catcagcgct tttttgacat tttcagtgtc atttttgtca ctcgcacgga ccatgaattc
    28201 actatttgtc gaagacgcaa caacaggtgc aattatgtca ttgataaata tcccacgcgc
    28261 attaatactc cccttaatcg tagacggatt atcgtaatat gagtcgataa ataccagtcc
    28321 acttgcattt atcgaaccgg taattaacag tggtgaatcc gtaactatat tcgttgccgt
    28381 cacgttaccg agaataaccc atggattaga aagagagaaa gtggaaatat tacctttgac
    28441 tgtcaaattg ccaagaacaa tcaatttttg attactctct atattacctt ctaccactaa
    28501 atcgccacga tgcactttcc agtccgatgc agataaaccg aaggggacaa gattcacgct
    28561 actgttgata gcttgttcaa cttgttgttt atcttcagcg gtagctgcag aaaaaaatgg
    28621 ttcaatatta actgcatttg caacacatcg aagttccgcc tgtactggaa gactcagcat
    28681 aaacagggga agtaaaccta tccagctgca aatcctgggc ttaaacatat gcactccatg
    28741 ccattaaaaa agaaacaaaa gggaaatgca tctttccctt ttgtatattc gttattctcg
    28801 cgaaatacca tccaacgtag acaacacttc ctcagaaaga tgcaattttt cagcagccat
    28861 attctcccgt aaatgcgcaa cagaagatgt ccctgggatc agcaaaatat tcggtgaacg
    28921 ttgtaacagc cacgccagcg ccacctgcat gagtgttgca cccaggctcg cagcaacatc
    28981 cgacagcgta gaggattgca gcggtgtaaa gcccccgagc gggaagaacg gcacgtaggc
    29041 aatgccgtca cgagccaaag catcaatcat tgcgtcatca gcacgatgag caatgttgta
    29101 ttcgttctgc acacagacga tttcggcaat cttgcgcgcc tctgcaacct gcgtcggtgt
    29161 gacgttgctt aggccaatat gttttaccag gccttgttgt tgcatctctg ccagcacggt
    29221 caggctggct tcaatcgatc cttccgctgg gccatgacca tcccccatca taatgcgcaa
    29281 gttaaccaca tccagtacgt ccaggccgag attacgtaga ttatcgtgca ccgctttttg
    29341 caactctgcc ggagaaaagg cgggcaacca ggaagcgtcc tctccacgcc gcgcaccaat
    29401 tttagtgaca attgtcaggt cgtcagagta aggataaagc gcttcgcgga taatctgatt
    29461 ggtgacgtgc ggaccataaa agtcgctggt atcaatgtga ttgacgccca atgccagcgc
    29521 ctcgcgcagc acagttatag cgacgtggcg atctcttggg ggaccaaaaa ccccaggacc
    29581 tgccagttgc atcgcgccat aaccaagacg gttaacggat tttgtaccga gagtaaatgt
    29641 attgctgctc atcgcaccct gccctgtaat aaaacggatt aagaaaaatc agtatagaaa
    29701 cgaaaagaga taaataatgc gagggcgtaa acaagattac tggatgagtt gctctattgt
    29761 cctgaaaaac acaaaacaaa acgcgtaaag cgccgcatcc ggcaataaaa aaaccgcaac
    29821 ctgtatcagc gttgcggttt aggtatcaca tgctaaaaat tagaatgttc agtatctgga
    29881 cgttactgtt tcggtcgcag attctcatca aataccagac gtttcctgtt cggttcaact
    29941 tcccgcaatg gctcaacttg atgcaacgtc tgcttacaac gagtcaccag cacctgatac
    30001 tcatgcgtac cctgcttttt ccatgccagc tcctgctcac tgagttcacg aatcgctttc
    30061 gccgggctgc cgacaatcag gtaattggca ggcatttctg ctttggcttt cacaaatgcg
    30121 gatgcgccaa caatgctgtt ctcgccaatc accgcgccgt ccatcactac cgcgttcatt
    30181 cccactaatg cattgcggcg gataatgcaa ccatgaagga tagcgctatg accaatatgt
    30241 ccatcttctt ccacgacagt atcctgctcg ggaaaaccgt gcataacgca attatcctga
    30301 atgttcgcgc catctttcac cacgatacga ccaaaatcgc cacgcaggct ggcatttggc
    30361 ccaacgtaaa cgcccttgcc tagaataaca tcgccgatca ataccgctgt cggatggaca
    30421 aaactctctt ctggcacaac cggagtcaga ccatctatct ggtaaattgg catatcgcct
    30481 cctgttcaat attcaaaccg ccaaaacgtt gaaaatacag gcttcccggc gcaggcagtt
    30541 caccgaccga ggtttcacct ttttcactaa cgaacgctaa agcagcaggc gctacgcgct
    30601 gataaatgtt gatacacagc tgacgcgccg tatgccctgc ccagtgcgcc ggaagcaact
    30661 cctctggcaa caatgggtct ttaaggacga cacggcgata aaaatggatc agtaaaagct
    30721 gaatatgaaa tgcacgctcc ggggttaact cgtctgctgc ctcttttaac agcggcacca
    30781 gcgggcggaa tgactgaata aaggtttcgt acatggcatt ttgttcagtt aaatgccagc
    30841 actcttccac tctggcacgc agtgctgcac gagaaagcgc cagtggtatt tgcgcttcaa
    30901 aacaaatcac gttatccgcc acccccgctt catgcaaaag tgtctgtaca tccgccagtt
    30961 tttgcgacgg cgatgccatc aggctgggtg ccagtgcgcc aaaaccttgc cagatcaact
    31021 gctttttgac atcagccagc gttgatttat ctaaaccttc cgagagcaac aggagccatt
    31081 taccatccca tgcaggttgc tctgcgcgat aaattttact ttctgcccgt cgcgtcaggc
    31141 gcaagccttt atcactgagg ctatagaaac tgcgtcgccc gatgcgggaa acatccagcc
    31201 agccttcttt attaagacga aacaaagcgg tgcgcacgaa acgctcacca aatcccagcc
    31261 cttccagcaa agcagccaga ctacccagcc agatttcacc accacgatgg gaaagcgaat
    31321 caccatacag agaggagatc aaagatgtgc cactgaccgg aacagcgttt acagcatgtt
    31381 ggataaaagt aacaagttta ctcattcgat tcagtctgtt gttattgttg tgatttatga
    31441 atcatagcac aggtcgtatc gacttctggc cctggcgctt tacctcacca gggccagacg
    31501 acacgtcagg caccaacaat attgcgcaga tcaaacaccc gacacgcctt gccttctgaa
    31561 cgcgggatac tgccacagtt aacgatcatc acatcggtag agatccccac catcgactta
    31621 atccgatggc gcagctggtg gcacacctgg caacgctgct catgcgtcaa tgtcaggctg
    31681 ctttctttca actccacttt cacagaaagt gaatcaagat gcccccggcg gttcacctcc
    31741 agttggtaat ggggcgataa atgttcgaat ttgacaatct cttcttccag ttgtgacggg
    31801 aagacattta caccgcgaat gatcaacatg tcatcgctgc gtccgctgat gcgatccatg
    31861 cggcgcatag tccgcgccgt tcctggtaac agtcgggtta aatcacgcgt gcggtaacga
    31921 atgaccggca atgcttcttt ggtcagcgtg gtgaataaca gttcgccatg ttcaccatcg
    31981 gcaagcggtg tgccgtcatg aggattaaca atttcaggat agaaatgatc ttcccaaatg
    32041 gttgggccgt cggtagtttc cagacactcc atcgccaccc ccggccccat cacttctgac
    32101 aggccataaa tatccagtgc ggtgatcccc agacgccgtt caatctcttt acgcatggcc
    32161 tgtgtccacg gctcagcacc aaacactcca acccgcagcg agcaaccgct ggcatcaccg
    32221 cccaactgcc gctccagctc ttcaatcagg ttaaggcaat aagatggcgt aaccatgatc
    32281 atatctggtt gaaaatcgcg gatcagttgc gcttgttttt ccgtctgccc accagacatc
    32341 gggatcaccg tagcgcccag acgttcggca ccatagtgcg cgcccagccc accagtaaac
    32401 aggccgtaac cataggcaac atgaattttg tctttgggcg tgccacctgc cgcacgcaag
    32461 gaacgcgcta caatattggc ccagttatca atatcgtttt gcgtatagcc gacaactgtc
    32521 ggtttacctg tggttcccga agaagcatga atgcgcacca cttgttccat cggcacggca
    32581 aaggtgtcga agggatagtt gtcgcgcaga tcctgtttgg tggtacaggg aaatttacgc
    32641 agatctgaaa gttccctgaa atcatcagga tgtacgcctg ctgcgtcaaa tttgcgccga
    32701 tacatcggga cattttcata cgcgtgtttg agcgtccatt tcagacgttg tgtttgcaac
    32761 gcctgtaact catcaacaga cgcggtttca atcgggtcaa gctttgtatt ggttatcatt
    32821 gtagggtact cgcaggttga tatgctcaaa cacgctccag aatcatggcg atgccctgac
    32881 cgacaccgat gcacatggtg cacaatgcgt aacgaccgtt acgccgatgc agctcatggc
    32941 tggcagccag tgccaggcgg gcaccactca ttcccaacgg atggcctaag gcgatagcgc
    33001 ctccgttggg gttaacatgt ggggcatcat caggcagccc caattcgcgt agtacaccca
    33061 acgcctgggc cgcgaacgct tcgttcagtt caatcacgtc catatcgtga atactcagcc
    33121 ccgcgcgttc cagcacccgg cgagttgcag gcaccggacc aagccccatc aggcgcggtt
    33181 ccaccccggc ggttgccatg gctacgattc gcgcccgcgg tgtcagtcct tgcgctgctg
    33241 ccatctgttc actggcaata atcaacgcag cggctccgtc attcaccccg gaggcattgc
    33301 ctgcggtaat caccccattg gcacgaaatg gtgcttttaa cccacgtaac tgttccagcg
    33361 tcgtttccgg gcgcagatgc tcatcatgtt gtatttctgt tacaacgcct ttcttgtttt
    33421 tcaacacaac cggaacaatc tcctcagcca gaatgcctga ggattgcgct tttgccgtac
    33481 gttgctgact gcgtagcgca aaactatctt gatcttctcg cgatattttt aacagttcag
    33541 ctacattctc tgccgtttcc ggcatgctgt cagttccaaa ttgttgagcc atgagcgggt
    33601 tcacaaatcg ccagccaata gtggtatcaa acatctcagc ctgacgagaa aatgcactgg
    33661 ttgccttgcc cataacaaac ggtgcccgag acattgactc cacgccacca gcgatcagca
    33721 aatcgccatc gcccgcttta atcgcccgtg cggcgaaccc cagtgcgtcc agcccggaac
    33781 cacacaagcg gttaatggtt gtgccggaaa cactctgcgg cagccccgcc agtaacgtcg
    33841 ccatccgggc tacgttacgg ttgtcttctc ccgcctgatt agcacagccg aggatcacat
    33901 catcgataca ctccgcatcg agacgcgggt ttcgcaccag cagctcccgc aaagggatag
    33961 cagccagatc gtcagcccga acacctgata atgccccacc atagcggcca attggcgtac
    34021 gaattccgtc acagataaag gcttcacgca tcaggcttct cctgtaatgg tgccgccgat
    34081 gcggtgagat ttaccgcgaa acagcgcaac cgttttttgt tgttggttaa caatttcgat
    34141 gtcgtaaaca ccggtttgct tgccctgatg acgtacctgc gcagtcgcag ttaaggtgtc
    34201 tccggcaaag cctggacgca aaaaatcaat cgtgcaggca gaagcgacgg ctgccagccc
    34261 ctggctattg caggcgtagg caaaggcagt atcagccagt gaaaatagct gcccgccgtg
    34321 gcaactttga tgaccgttaa gcatttgtgc agtgacggtc atggtcacta cagcaaagcc
    34381 ttcatccatt gagataatgt cgatgccaag cgctttggcg caggcatcgt tctcatacat
    34441 tgcatgggca ttttgccagg ccttatgact cataaccgct ctccagaagc gctcgttggc
    34501 gcagcaatga acatgggcga tagcgttctt cgccatagtg atgttgtaga ttttccagga
    34561 gccttaatat tcgctgccag ccaagttgcg ctccccaggc aagcgggcca tatggataat
    34621 tcaccccaag acgcatggcg gtatcgatat cctgttcaga ggccacgcct ttttgcagcg
    34681 catcaagggc ttcattgatg atcattgcta ccgttcgcca aatcagcatt cctgggtaat
    34741 ctgcaatttg cagcactgtt ttgccctgct gttgcaggta ataaatggcc ttgcgggtcg
    34801 ctgagtccgg gttcaccgct gccgcagcaa tggtcaccac cttgcccgcc attttgtcga
    34861 ccactaccac cgggcgtgcc agtcgtattg ccagcgcctg tgccgtctcg ccttgtgtct
    34921 caatcaataa aacatcgtca atttccgtga cgccgtcact tttcttttct actttcattg
    34981 ggctaaaact gtcgcttacc gcttccaggc caacaactgc ctcacgttcc gcgcgccagt
    35041 cgtacacgcc cagcccactt ttcttgccca accgtccacc aatcaccagt tcctgttgca
    35101 ccagcgaagg taaaaaacga cgctcctgcc agaaagcgtt aaacaccgaa caggtgacag
    35161 caaaattgac gtcctgacca atcagatcgg ttaattccag cggccccatc gggaaaccag
    35221 cgccatcgcg aagtgcagcg tcaatcactt ctggtgcagc aacctgctct tccagtgccc
    35281 gccaggcctc ggaataataa ggacgcgcca cacggttaac gataaatcca ggggtcgaat
    35341 gacagcgcac aggctgctta ccccaactca acgttagttc acacaactgc tcaacaactt
    35401 ccgctgccgt tgccagccca ctgaccacct ccaccaactt catcaccggt gccgggttaa
    35461 aaaaatgcag ccccgcaaca cgttcaggat tttttacctc cgcagcaatc gcggttatag
    35521 agattgacga agtgttagtg gtcaatagcg tttgtggcgg gcaaacttcc gccagctgtg
    35581 caaagagcgc ttttttgact tccagacgtt cagacgccgc ttcaatgacc aggtccgcag
    35641 ctgccagcgc gtgaatatcg gtcaccggga tcaggcgttt caatgtgcgt tcacaggttt
    35701 cagcagtcag ttttccccgc gtcacgcgtg aatttagccg cgcgtgtatc ccgtcgattg
    35761 cgcgggtcag cgcttcagca gaaatgtcat acagtaaaac ctgatgtcca tgactggcgg
    35821 caacttcagc aatgccagcc cccatggtgc cgctcccaat cactgccaca gtttgcacat
    35881 ttatcatcat gctatttccc cgtgaactgc ggtgagcgtt tagccaggaa cgcactgaca
    35941 ccttcacgat aatctgcgct ccgtccggca agtcgctgat agtcgcgttc cagatccagt
    36001 tgcgtatcga gcgtattggt ttcagcgcta tttatcgctt gcttgataag tccaagacca
    36061 aatgtcggtt gtgtcgccag atgccgtgcc agctgttgcg cggtatctgc cagcgtttca
    36121 tcatcaacaa cctgccagat catcccccat tcgtgcgcct gttcagcact cagttgattc
    36181 cccagcagtg ccagccccat ggcgcgcgct cgcccagcaa cgcgtggcag taaccaggtt
    36241 ccaccgcaat cgggtactaa acctaactta ctaaacgcca tgacgaattt tgctgaacgg
    36301 gcagcaataa cgatgtcgca tcccagtgcc agtgttgcgc cagcccctgc cgccacgcca
    36361 ttgactgcac agatcaccgg ttttggcagt tttgccaggc gacgtaccag tgggttatag
    36421 aaacgttcaa ctgacatccc taaatccggt gcggggcctg tgggatcaac gttacgatcg
    36481 ttaagatcct gaccagcaca aaatccgcgc ccggcacctg taagtaacag gcaacggata
    36541 gtgtcgtcgc gctcgacctg tttcaggcac tctgccagtt gtgcgtgcat ctcatcatta
    36601 aaactgttca ggcgttccgg gcggttgagc gttagtgtca tcacgccctt ttctacatga
    36661 ctgaggatga attccatcat tagcgtcctt taaagtcggg cgtgcgtttt tgcaagaaag
    36721 cggagatgcc ttcatgacga tcttctgttg ccgccagcaa ggtgaataac tgtcgctcct
    36781 gggcaagtcc ggcttgcaaa gccacttcct gcgactggcg cagcgcttgc tttgccgctt
    36841 gtaaggccag cggcgagtga cgtgccattt tcgatgccag ctgtaaggcg tattcgaggg
    36901 ttaaatcgct ggggaagacg tcgctaacca gcccggcctg ctgtgcttgc tgagcggtga
    36961 tactttctcc gctcagcacc attttgctgg ctaacgattt accgacacta cggattaaac
    37021 gttgcgttcc tcctgcgcct ggcatgatgc cgagagtgat ttccggcaaa ccaaaacgcg
    37081 cgttctctcc ggcaaccacc acatcgcaca acaatgccag ttcgcaaccc gcaccaagcg
    37141 cgtaaccgtt gacggctgcg atgagaggtt tgttgaaggc ctgcaatcgc gcccatagct
    37201 gcgggcgtgt atcgtttaag gtggccgcga gatctttttc tgccatttcg ttgagatcgg
    37261 ccccagcggc aaaaaagcgt gcattaccgg taatcacaca gaccgaaatg ctgctatcgg
    37321 tagccgcagc ttccagttca tttaccagtt gcgtcagaag ggcattattt agcgcattac
    37381 gtgcggcggg acggttaagg gtcagcaaca atacttgttg ctgacggctg acgatcagtt
    37441 cgctcatgcc atccccttcg cgtcaaagtc aaccaccaca tcgctggtca gcggcagtgc
    37501 ctggcaactc aacacataac ctgcggccag ttcatccggt tccagactgt aattggtttc
    37561 catcgccact ttgccacgca gcactttgca tttgcaggtc gcacagacgc cgcctttgca
    37621 ggcatagggc agatcagccc cctggcgcaa tgccgcatcg agaatgcttt catcgtcggc
    37681 attaagcacg atttcccgat cccgcccatc ctgacgtaca gtcacttttt gtccgtcact
    37741 ttgcacgtta acgctacgtt tgacgcgcgt gccaggcgta ttaaaccgct ccagatgaat
    37801 ggttttatct ggcattccca gtgcttttaa ggcggcttcc gtctcatcca tcatcgccgc
    37861 cggaccacaa ataaatgcct catcataaag acgaaaatta atgagcgagg ccccaagtga
    37921 ctgtaatttt tcaccgtcaa tacgcccgtg aagcagatcg ctgtcgaggg tttcctgact
    37981 gaaaatgcac aacaactgta aacgctgagg atatttgtct ttcaggtctg ccagtgcctg
    38041 gcgaaacatc atgctctggc tggtacggtt accgtagatc agggtgaact gactttcagg
    38101 ctcggtttgt aaagtggtgg cgataatcgc cagcattggc gtaatacctg atcctgctgc
    38161 aatagccaga tagcggccct ggcgttcggc ctgtggctga tagccgaaat gcccctgcgg
    38221 caccatgacc tccagcgtca taccctggcg gatgtgttcg cgggcatagc gggagaaacg
    38281 tccgccttca atggctttca ccgccacact aatttcgcca ggcaggtagc tgcggcaaat
    38341 ggagtaacaa cggcgtaatt cttcaccatc aagactggct tttaaggtca aatgttgacc
    38401 ggggcgaaag cgatacgcct cctgcaaagg ctggggcacc gcaaaggtaa tggtcaccgc
    38461 atcacgggtt tccggctcga cttttgccac tgttaaggaa tgaaacgttg tcatggcatc
    38521 ctcaaataca tttgaaataa tcgaaaggtt cgcggcaact atcgcagcgg tacaatgctt
    38581 tgcaggccgt ggaaccaaat tcactgataa gtgtggtatg gacgctggcg cagcgcgggc
    38641 aacgtacttc tggcggcaaa tgggcatggc aactgtgtcc ggcgggcggg ctaatgccat
    38701 actgtcgcag acgctcacgg gcatcggggg tcatccagtc ggtggtccat gccgggtcga
    38761 gttgcagcac aacctgaacg ggggtaaacc cgtgggttgt cattgcctca cgtatcgcgc
    38821 caatcaaatg ttccgttgcc ggacaaccgg aataagtcgg cgtaaagccg atcacccatc
    38881 cttctcccat ctgtgtcaca ttacgcacca tgcctaaatc ggtaatggtc agcaccggga
    38941 tctccgggtc cgggatctgg ctgagtagct cccatatctc atgaacctgt ggcggtgcaa
    39001 tggtggccag acgttgcata cccatctcct ctgttaccat tgctgaccgg gcaagacacg
    39061 ctggagatac tgcatttctg ccagcatggg tccaaggtgt tcggtatgca gtcctttttt
    39121 accgccagtg cgatacgcct gctcttgcgg tacgttcaat gtggcttcgt tgattccggc
    39181 aaaaacttcg gcttcccacg ctgcgcgtaa agtgcgtgga tcaaccgcaa taccctcttc
    39241 actcagtgca atatcaatct cgtcggcatc gaacagttcg gcagtaaaac gccacaactt
    39301 gtcgatggcc tgctgcatct tttgccctga tacgtcagta ccattgccca gtcgctccag
    39361 ccagccacga ctaaaacgca ggtgataacg cgcttcttta attgctttgg cagaaatcgc
    39421 cgccagttgc ggatcacggc tttccatcag acgggtaaag agcgccatat gccatgcatc
    39481 gatgaaatac tggcgtgcaa tggtgtcggc aaaattgccg tttggttgtt caaccaacaa
    39541 taagttgctg aactggcgct cgtctcgggt gaaggccagg gtatcttcat caccttctcc
    39601 cgctaattca gcggcatacg ataagaagtt acgtgcctga cctaataaat cgaggccaat
    39661 gtttgccagt gcgagatcga tttccagttc cggtgcgtga ccgcaccatt cccccaaccg
    39721 ctgggagagc accagacagt tatcgcccag gcgcaaggtg taagccgtta actgattcat
    39781 ttccgacctc acatgtgctc aatgccatca gggatggtgt aaaacgttgg atggcgatag
    39841 accttgcttt cagccggatc aaaaaattca ccgcgttctt ccggttgcga ggcaacaatt
    39901 tcactcgcct tcaccaccca aattgaacat ccttcgctac gacgggtgta agcatcacgg
    39961 gcattttcca gtgccatccg ctcatcggca gcatgtaaac tgccaacatg gcggtgtgat
    40021 aagccctgtt tgccacgcac gaaaacttcg tataacggcc agtaaacatt actcattttg
    40081 aatctcctta tgcgacctta cgggcaagtt gtttttgtgc atgggccagc gctgcttccc
    40141 gtacccaggt accttcttcc caggctttac gtttggcgtc gagcctctcc tgattacaaa
    40201 ttccgcgacc gttaattact tcgttaaact cctgccagtc gatctcacca aagcggtagt
    40261 gaccgctttc agtgtcaaaa tgcagatccg ggtcaggaac ggtcataccg agcatttcaa
    40321 cctgtggaat ggtgttatcc acgaaacgct ggcggagttc gtcgttggtg aaacgtttga
    40381 ttttccaggc gagacttctg gcgctgtttg gcgagttatc atcgtttggc ccgaacatca
    40441 ttaaggctgg ccaccagaaa cggttaatgg cgtcttgcaa catctgcttt tgagcttcgc
    40501 tgccctgcgc cagtgccatg caggcttcaa acccctgacg ctggtgaaaa ctctcttctt
    40561 tgcagatttt caccattgct ctggcatacg ggccataaga agtgcggcat aacgccacct
    40621 gattgacgat cgctgcgcca tccaccagcc agccgataac accgatatca gcccaactca
    40681 atgtcggata attaaagatg gaggaatatt tcatccgtcc gtcgagcatt ttttggtaga
    40741 tgtcttctct ggcgcagccc agtgtttcgg cggcgctata gagatacaga ccatgaccgg
    40801 cttcatcctg cactttggcc aacagaatgg ctttacgccg caaggttggt gcgcgagtga
    40861 tccagttacc ttcaggcaac ataccaacaa tctcggagtg cgcatgctgc ccaatctggc
    40921 ggatcagtgt ctttcggtaa gcatcgggca tccagtcctg cggctcgata gccgtttcct
    40981 gggctatccg ttgctcaaag cgttcttctt gggtcacgtc atcaccttta cgattccagt
    41041 tttcgtgaat taattaaaaa cacgaatcac gttgttttat gaaagttaca caatggttaa
    41101 ctaaaatcac aaattttgta tgtttgaact gtgacggatt tcgcacccta tttttataaa
    41161 tcattattta tcagtgcatt aatgttttcc gcagttgcta tacagatcgc atagttaaca
    41221 tttcgttaaa agatcccttg ctttttatga ttcgcgattt aactattagc aacagaaatg
    41281 tgaaacatct ggagagtagc gatgcagcag ttagccagtt tcttatccgg tacctggcag
    41341 tctggccggg gccgtagccg tttgattcac cacgctatta gcggcgaggc gttatgggaa
    41401 gtgaccagtg aaggtcttga tatggcggct gcccgccagt ttgccattga aaaaggtgcc
    41461 ccagcccttc gcgctatgac ctttatcgaa cgtgcggcga tgcttaaagc ggtcgctaaa
    41521 catctgctga gtgaaaaaga gcgcttctat gctctttctg cgcaaacagg cgcaacgcgg
    41581 gcagacagtt gggttgatat tgaaggtggc attgggacgt tatttactta cgccagcctc
    41641 ggtagccggg agctgcctga cgatacgctg tggccggaag atgaattgct ccccttatcg
    41701 aaagaaggtg gatttgccgc gcgccatgta ctgacctcaa agtcaggcgt ggcagtgcat
    41761 attaacgcct ttaacttccc ctgctgggga atgctggaaa agctggcacc aacgtggctg
    41821 ggcggaatgc cagccatcat caaaccagct accgcgacgg cccaactgac tcaggcgatg
    41881 gtgaaatcaa ttgtcgatag tggtcttgtt cccgaaggcg caattagtct gatctgcggt
    41941 agtgcgggcg accttttgga tcatctggac agccaggatg tggtgacttt cacggggtca
    42001 gcggcgaccg gacagatgct gcgagttcag ccaaatatcg tcgccaaatc tatccccttc
    42061 actatggaag ctgattccct gaactgctgc gtactgggcg aagatgtcac cccggatcaa
    42121 ccggagtttg cgctgtttat tcgtgaagtt gtgcgtgaga tgaccacaaa agccgggcaa
    42181 aaatgtacgg caatccggcg gattattgtg ccgcaggcat tggttaatgc tgtcagtgat
    42241 gctctggttg cgcgattaca gaaagtcgtg gtcggtgatc ctgctcagga aggcgtgaaa
    42301 atgggcgcac tggtaaatgc tgagcagcgt gccgatgtgc aggaaaaagt gaacatattg
    42361 ctggctgcag gatgcgagat tcgcctcggt ggtcaggcgg atttatctgc tgcgggtgcc
    42421 ttcttcccgc caaccttatt gtactgtccg cagccggatg aaacaccggc ggtacatgca
    42481 acagaagcct ttggccctgt cgcaacgcta atgccagcac aaaaccagca acatgctctg
    42541 caactggctt gtgcaggcgg cggtagcctt gcgggaacgc tggtgacggc tgatccgcaa
    42601 attgcgcgtc agtttattgc cgacgcggca cgtacgcatg ggcgaattca gatcctcaat
    42661 gaagaatcgg caaaagaatc caccgggcat ggctccccac tgccacaact ggtacatggt
    42721 gggcctggtc gcgcaggagg cggtgaagaa ttaggcggtt tacgagcggt gaaacattac
    42781 atgcagcgaa ccgctgttca gggtagtccg acgatgcttg ccgctatcag taaacagtgg
    42841 gtgcgcggtg cgaaagtcga agaagatcgt attcatccgt tccgcaaata ttttgaggag
    42901 ctacaaccag gcgacagcct gttgactccc cgccgcacaa tgacagaggc cgatattgtt
    42961 aactttgctt gcctcagcgg cgatcatttc tatgcacata tggataagat tgctgctgcc
    43021 gaatctattt tcggtgagcg ggtggtgcat gggtattttg tgctttctgc ggctgcgggt
    43081 ctgtttgtcg atgccggtgt tggtccggtc attgctaact acgggctgga aagcttgcgt
    43141 tttatcgaac ccgtaaagcc aggcgatacc atccaggtgc gtctcacctg taagcgcaag
    43201 acgctgaaaa aacagcgtag cgcagaagaa aaaccaacag gtgtggtgga atgggcggta
    43261 gaggtattca atcagcatca aaccccggtg gcgctgtatt caattctgac gctggtggcc
    43321 aggcagcacg gtgattttgt cgattaatcg gtgaatgaag ggcaacggcg aatagttgcc
    43381 cttttatttc actaaatttt gtgacgttgt cacattatgc atgatgtgta catctatttt
    43441 cagggcatcc actgtatgaa aagctggcac acctgccaaa caacctggca ggtgcaggca
    43501 atccccttta catcagtact gataatgtga acctgaccaa accgcccaca gagcgtggtt
    43561 gctaacaaga acacaacatc tgacgaggtt aataatggga agcccctctc tgtattctgc
    43621 ccgtaaaaca gccctggcgt tggcagtcgc cttaagtttc gcctggcaag cgccggtatt
    43681 tgcccacggt ggtgaagcgc atatggtgcc aatggatgaa acgcttaaag aatttggtgc
    43741 cgatgtgcag tgggacgact acgcccagat ctttaccctg attaaagatg gcgcgtacgt
    43801 gaaagtgaag cctggtgcgc aaacagcaat tgttaatggt caacctctgg cactgcaagt
    43861 gccggtagtg atgaaagaca ataaggcctg ggtttctgac acctttatta acgatgtttt
    43921 ccagtccggt ctggatcaaa cctttcaggt agaaaagcgc cctcacccac ttaatgcgct
    43981 aactgcggac gaaattaaac aggccgttga aattgttaaa gcttccgcgg acttcaaacc
    44041 caatacccgt tttactgaga tctccctgct accgccagat aaagaagctg tctgggcgtt
    44101 tgcgctggaa aacaaatcgg ttgaccagcc acgcaaagcc gacgtcatta tgctcgacgg
    44161 caaacatatc atcgaagcgg tggtggatct gcaaaacaac aaactgctct cctggcaacc
    44221 cattaaagac gcccacggta tggtgttgct ggatgatttc gccagtgtgc agaacattat
    44281 taacaacagt gaagaatttg ccgctgccgt gaagaagcgc ggtattactg atgcgaagaa
    44341 agtgattacc acgccgctga ccgtaggtta tttcgatggt aaagatggcc tgaaacaaga
    44401 tgcccggttg ctcaaagtca tcagctatct tgatgtcggt gatggcaact actgggcaca
    44461 tcccatcgaa aacctggtgg cggtcgttga tttagaacag aaaaaaatcg ttaagattga
    44521 agaaggtccg gtagttccgg tgccaatgac cgcacgccca tttgatggcc gtgaccgcgt
    44581 tgctccggca gttaagccta tgcaaatcat tgagcctgaa ggtaaaaatt acaccattac
    44641 tggcgatatg attcactggc ggaactggga ttttcacctc agcatgaact cgcgcgtcgg
    44701 acctatgatc tccaccgtga cttataacga caatggcacc aaacgcaaag tcatgtacga
    44761 aggttctctc ggcggcataa ttgtgcctta cggtgatcct gatattggct ggtactttaa
    44821 agcgtatctg gattctggtg actacggtat gggcacgcta acctcaccaa ttgctcgtgg
    44881 taaagatgcc ccgtctaacg cagtgctcct taatgaaacc atcgccgact acactggcgt
    44941 gccgatggag atccctcgcg ctatcgcggt atttgaacgt tatgccgggc ctgagtataa
    45001 gcatcaggaa atgggccagc ccaacgtcag taccgaacgc cgggagttgg tggtgcgctg
    45061 gatcagtaca gtgggtaact atgactacat ttttgactgg atcttccatg aaaacggcac
    45121 tattggcatt gatgccggtg ctacgggcat cgaagcggtg aaaggtgtta aagcgaaaac
    45181 catgcacgat gagacggcga aagatgacac gcgctacggc acgcttatcg atcacaatat
    45241 cgtgggtact acacaccaac atatttataa tttccgcctc gatctggatg tagatggcga
    45301 gaataacagc ctggtggcga tggacccggt ggtaaaaccg aatactgccg gtggcccacg
    45361 caccagtacc atgcaagtta atcagtacaa catcggcaat gaacaggatg ccgcgcagaa
    45421 gtttgatccg ggcacgattc gtctgttgag taacctgaac aaagagaatc gcatgggcaa
    45481 tccagtttcc tatcaaatta ttccttatgc aggtggtact cacccggtag caaaaggtgc
    45541 ccagttcgcg ccggacgagt ggatctatca tcgtttaagc tttatggaca agcagctctg
    45601 ggtaacgcgt tatcaccctg gcgagcgttt cccggaaggc aaatatccga accgttccac
    45661 tcatgacacc ggtcttggac aatacagtaa ggataacgag tcgctggaca acaccgacgc
    45721 tgttatctgg atgaccaccg gcaccacaca tgtggcccgt gccgaagagt ggccgattat
    45781 gccgaccgaa tgggtacata ctctgcttaa accatggaac ttctttgacg aaacgccaac
    45841 gctaggggcg ctgaagaaag ataagtgatt gtttcagaca aaaaaacggc accaggtgcc
    45901 gttttttact tatgagcgaa ccagattaat accgtacaca caccgactta gtttcacacc
    45961 aaccgtccag ccagtcgggg ccaaaatcac ggcccgttcc tgactgcttc atcccaccaa
    46021 acggtaagtt agcgtcaatt aaggtatggc tgtttaccca caccgtacct gcctgtaagc
    46081 gatcgctata ttccagagcc tgggagagat tttgcgtcca gacactggca gttaagccat
    46141 attccgtgtc gtttgccagt tgtaacgcct cttctccatc cgctactcgc accaggttta
    46201 ccaccggacc aaacacctct tcacgagtta aacgcaattt agcatcggga tttaccacca
    46261 gcgttggcgc aacataatac ccctctccgg ctggtccatt cgacccgcga atcagctctg
    46321 cttgctgtgc ctgcgcatcg tcgaggaatg aacacacttt gtcgcagtgc gcacgagaaa
    46381 ccaaagggtt aatctgtgca acaggtgaca tccccggtcc cacttgcaac gattttaccg
    46441 cctgctcaaa tccactaacc agcgtgtcaa acaacggcgc ttcaatataa attcgcgaac
    46501 tggcggcgca tacttgccct tgattcagga agcttccggt catcaagcct tcaataaccc
    46561 attgcggatc agcatctttt aatacaattg ccgggttttt accgcccagt tccagcgtta
    46621 cacgcgttaa gtgatcagca gcagttctgg caataccttt tcccgtcgcg gttgaaccgg
    46681 taaaactgat tttcgcaaca tgaggatgtg acgtcagggc cgcgccgcat acagcacctg
    46741 acccggtgac gacattaaaa acgccatcag ggataccagc ctcgctggcc agttccgcca
    46801 cgcgcaacat cgtcagtggc gtggtttccg aaggcttaat cacgattgaa cagcctgctg
    46861 ccagtgctgg catcaccttc cacataccaa tcatcaacgg aaagttccat ggcacaattc
    46921 ccgccactac gccaaccggc tctttacgcg tccaggcctg ataacgcgcc ccctggggta
    46981 agggaatcga caagtccagc gttttacccg cgattttggt cgttaacccg gcggtataac
    47041 gcatccagtt cagcgtacag cccacttcaa aagcacggga aatggcaatt gacttgcctt
    47101 gctccagggt ttccagttgc gccagctcct cactgtgctg ttccaccaga tcggcaaaac
    47161 gtagcagaat acgttcacgc tctgcgggta atcgcccggc ccagcgacgc gagacaaagg
    47221 cccgccaggc agacatgact gcgttatcta catccgcttc gttggcatca gcagtagacg
    47281 caatttcttg cccggtggcc ggatcaaaga tcgccaaccg tttttcactt tgtgcggggc
    47341 caggacgacc atcaatataa agaccgtgtt gacgatcgag aaactgttgg acctggctta
    47401 atactgctac atgcggctct gtcatcactt ttccttatta tttacccagt gtgatttcag
    47461 tgtacgcagc gacaagaaaa gtcacttttt tcgctgtgac attcgctttg tcagctgtga
    47521 caagctccgc aaatcgtgac aataaccgct tatgtctcta tgcctgacaa gacttatttc
    47581 ctatagtcag gctcattagc ttcgtttatt gcaacacaaa tgcaacaata aaaatacatt
    47641 tcacagagcg aaaacgtgcc atgaaccccg cagtggataa tgagtttcag caatggcttt
    47701 cccaaatcaa tcaggtatgc ggaaatttta ccggacgcct gctgactgag cgttacacgg
    47761 gtgtactgga cacgcatttt gccaaaggac taaagctgag taccgtgaca accagcgggg
    47821 tgaatttatc ccgcacctgg caggaggtaa aaggcagcga cgatgcctgg ttttacaccg
    47881 tttttcagct tagtggtcag gcaataatgg agcaggatga gcgtcaggtg cagattggcg
    47941 ctggcgatat tacgttactc gatgcctcac gcccctgttc gctttactgg caggagtctt
    48001 ctaaacagat ttcattactt ttgccacgca ctctgctgga acaatatttt ccccatcaaa
    48061 aacctgtctg cgcagaaaga ctggacgctg acttacccat ggtgcaactc agtcatcgcc
    48121 tgttacagga gagcatgaat aatccggcac tttctgaaac agaaagtgaa gctgcgctac
    48181 aggcgatggt gtgtctgctg cgcccggtac ttcatcagcg ggaatctgtt caacctcgtc
    48241 gtgaacgtca gtttcaaaaa gtggttacgt tgatagacga taatattcgc gaagagatat
    48301 tacgcccgga gtggatagcc ggagagacag gtatgtcagt acgtagtttg taccgaatgt
    48361 ttgccgataa aggtttggtg gtcgcgcaat atattcgtaa ccgtcgtctc gatttttgtg
    48421 cagatgcgat tcgccatgcc gcagatgatg aaaaactggc aggcatcggc tttcattggg
    48481 gattttctga ccagagtcat ttttcaacgg tatttaagca acgctttggg atgacgccag
    48541 gcgagtatcg acgtaaattc cgctaactaa ttggcgttgt gtgtactgca acgccaatta
    48601 gttaaaacag atatttgact atgcattttt cagggcgaaa ggcaatttga caggagtttt
    48661 tgaatattca ccttgtttca ggaaaataga tcaatcgctt tctcctgtag cgttatcccg
    48721 atattgcggt tttgctatta aaattttcgc acccatttac cgtttatccc ttgtacatat
    48781 tcccccggtt tggcccgagc tacgagcttt tgcccggcga gtttcgcgat atcatctaca
    48841 gacacattat tttgctttgc cagttgttgg taactcgctt tacgttcggc attaatgtcg
    48901 gttactaatt tctctgtttc agcatccgtt ttcagcgcaa ccagataacc ataaaatgtt
    48961 tcacctaccc gcccctgagt tctggcttca tccagagtca atgccattac attgctgctt
    49021 accagcccaa caagaaacgc acaaagaagt aatgttttct tcatcatcgc ctcagaaaag
    49081 atcgctacga gtttcaagca gctcttcgac atctttgtct gccttgatga tgatctcatg
    49141 ctcaatttta acgttcatat tgatagtgat cggttccttg ggcgcagcga cttcaatacg
    49201 cggcgtacag gcagccagca taaaagatga cgtcaacgca gccagtaaaa ttttcattgt
    49261 ttttcctcac actcttttcc ttgcggacag tcatttcccg gcagacgtgc gttctgctcc
    49321 aaccatgcct ggagattatc gccaaagcgt aaactgcgcc acagcgtaaa aatattctct
    49381 tcatgatagt aattaaggtt taccgtaccg cttttaccat caacgcgact ggtacctgta
    49441 atgttggcct gcatggttaa taaaccgaga ttatctaagt taatttttgt cgatgaccgg
    49501 ctaatttcca tatagcgcaa ccagttaatt gctgaacccg cagtcatatt gtctttcacc
    49561 accgcatctg cggtgtcttt atccagccgc aaggtcattg gcccgctatt tgccagccaa
    49621 ccatctttca ctatccattt ttcgttgttt agccataacg gcaatgcacc actaaatgcc
    49681 ccggacatgg cgaattgttt cggattgacg gcgctaacca gttcgctgga tgagagatta
    49741 ttcagccgca acagcgccgg gtcatgttgc ggcatacgta attgtttcat cagtacgtta
    49801 ccgcctaaca catcgacgct aacatcagtc aacagcaagg gttcttcttc agtccacgga
    49861 taacgccctt gcaaatcagc cgtaatattt ttcgctgtca ccagattaat cacttcggca
    49921 attcgcaacg taacggggcc gcgagtcccc agatgccagg ctccatcggc aaaacggaaa
    49981 ggcaggacaa aatcgacacc gttaacctga ttatctggca tccaggcact accgcctttc
    50041 aacacgccgt gtcctcccgc gcggaatcct tgttcaggcg cagcagaaaa tgcaacctga
    50101 gcatacagtt caccatcgcg taagttcatc ttccagtcgg gtggcaccag cggctggaat
    50161 acagtcagtg attgttttgg ccaccaggcg ttaccgcgca gacgaatacc gtcccagcga
    50221 ccatttaccc gaaccgggcc aatctcacca gcatgtaaat cgcctttaaa gaggaaatac
    50281 gtcggatcgc gcccatcgac gctaaatttt aaggttgatg gcggtaacac actgccgcca
    50341 gtgaacagcg tttgcccggc gtccagtgac agcgcgccgc tgaaggaggg atgctgtgcg
    50401 tcacgtaccc agacgacggg cttgtcgaga attaatcgcg gcttttctac cgtcatcgta
    50461 ccgtattgta actggtcgaa accggtggag agatcggtca gcgtaatcgt gctgtcatgc
    50521 cactcacctt ttcctgcgac atcccaggtg gcattcatcg gtgtaaaact gccttttccc
    50581 cagtagcgcc actgccagcg gccagcgtca gggagaaaat cattcgccag cccatccata
    50641 tgcagcacga aatcgcccag ttcattttca tgcgcctgca aaatggcctg caaacgtccg
    50701 tcaacaccac gttgggtgac ttttacaccc gctaaaggcc agcggatttc atcgatgtcc
    50761 agcgaatcga tgactcttcc cttcgaacgt aataacgcgc cgggctcaaa ggccagcgtt
    50821 gggtcagtca gacttccact tagctgcgcg ggtaagcggg catataaaat gaggtccgcc
    50881 tgtttcgctt caccggtcag ttgcagtggc atctgacttt tttccatgct taattttcct
    50941 ggaccaaaat taagcaccgc gttgccttta cccgcttgcc cctgggtcag cacactcagt
    51001 cgcccgctga ccagagcgtt ctcgagccct gcctgccagt tgtcgacttt gacacccagt
    51061 cggccactca aaggaaaacc tgcatacggc cagctccagc gaccatcgct tacggtcaat
    51121 tgttgacgag taatttgcca cggcaaatcg agcaacggat cgccgttatc ccgtgccagc
    51181 acaatcaatt gcccgctatt ttcctgccag tccagctctg catccaccag tgacggttcc
    51241 tgcggcaagt ttaacgtcgc agtagcatgc ccacttacag gaagtccatc cggcacgagc
    51301 ggcatagtaa attcccccac cagtttaacc ggcggctgat tttcaaacgc gacgacatcc
    51361 agttcgctga ctgtaagttg ttgccctttc agctggcctt gaaatttaac tttttcgccc
    51421 tgataacgca gttgctggat atcagaggtt aatgcgagag agagttttcc ctgccattcc
    51481 tgccagggag aaaaaatcag tttatcgata ttgatccagg tgttaggcag catggactgc
    51541 cactgcgcga gggtttttgg agcagctggc gattgctccg tctgcggcaa tttcgccaga
    51601 caagcagaat caagttctac cgtgccgacg ttcaataacc agcggctggg atgtgaaagg
    51661 ctggcgttgg tgatatgcgc aagctgacaa tctcccacca gataacggag atcgggaatg
    51721 attaaacctt tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt cccgagcggt
    51781 agccagatgc ccgccagagt gggaacccac agcccgagcg tcatcaacag cgtcaacggc
    51841 acaagaataa tcagtaataa cagcgcgaga acggctttat atttacccag catgggtagt
    51901 taatatcctg atttagcgaa aaattaagca ttcaatacgg gtattgtggc atgtttaacc
    51961 gttcagttga aggttgcgcc tacactaagc atagttgttg atgaattttt caatatcgcc
    52021 atagctttca attaaatttg aaattttgta aaatattttt agtagcttaa atgtgattca
    52081 acatcactgg agaaagtctt atgaaactcg ccgtttatag cacaaaacag tacgacaaga
    52141 agtacctgca acaggtgaac gagtcctttg gctttgagct ggaatttttt gactttctgc
    52201 tgacggaaaa aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg
    52261 atgacggcag ccgcccggtg ctggaagagc tgaaaaagca cggcgttaaa tatatcgccc
    52321 tgcgctgtgc cggtttcaat aacgtcgacc ttgacgcggc aaaagaactg gggctgaaag
    52381 tagtccgtgt tccagcctat gatccagagg ccgttgctga acacgccatc ggtatgatga
    52441 tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct aacttctctc
    52501 tggaaggtct gaccggcttt actatgtatg gcaaaacggc aggcgttatc ggtaccggta
    52561 aaatcggtgt ggcgatgctg cgcattctga aaggttttgg tatgcgtctg ctggcgttcg
    52621 atccgtatcc aagtgcagcg gcgctggaac tcggtgtgga gtatgtcgat ctgccaaccc
    52681 tgttctctga atcagacgtt atctctctgc actgcccgct gacaccggaa aactaccatc
    52741 tgttgaacga agccgccttc gaacagatga aaaatggcgt gatgatcgtc aataccagtc
    52801 gcggtgcatt gattgattct caggcagcaa ttgaagcgct gaaaaatcag aaaattggtt
    52861 cgttgggtat ggacgtgtat gagaacgaac gcgatctgtt ctttgaagac aaatccaacg
    52921 acgtgatcca ggatgacgtg ttccgtcgtc tgtctgcctg ccacaacgtg ctgtttaccg
    52981 ggcaccaggc attcctgaca gcagaagctc tgaccagtat ttctcagact acgctgcaaa
    53041 acttaagcaa tctggaaaaa ggcgaaacct gcccgaacga actggtttaa tcttgccgct
    53101 cccctgcaat ccaggggagc tgattcagat aatccccaat gacctttcat tctctattct
    53161 taaaatagtc ctgagtcaga aactgtaatt gagaaccaca atgaagaaac tagccgcgtt
    53221 tgttgcgcta agcctgctga tggcgggatg tgtaagtaat gacaaaattg ctgttacgcc
    53281 agaacagcta cagcatcatc gctttgtgct ggaaagcgta aacggtaagc ccgtgaccag
    53341 cgataaaaat ccgccagaaa tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg
    53401 taaccgcttt agcggtgaag gcaaactgtc taatggtgaa ctgacagcca aagggctggc
    53461 aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa ctcgataaca ccattagcga
    53521 aatgctgaaa gaaggtgcac aagtggatct gaccgcgaac cagttaacgc tggcgaccgc
    53581 gaaacagaca ttaacttata agctggcgga tttaatgaat taatagctgc cacagctccc
    53641 ggcggcaagt gactgttcgc tacagcgttt gccgttgggt aatgcacaca tcccaatcgc
    53701 cgtaccatcc agttgacggg caacagaaag cgaaccgccg atcattgcac aatttgcttc
    53761 tccactactg gacatcgacg cttttaaacc tggcgctacg tgcgccgcag tcgcctgctg
    53821 aacaggttca ctactacacg ccgacaacaa taaagcggca caccctaccc aaaacgctgc
    53881 tcgcatcttt tcttcctctg atcttcaagc caaacgacac cgccataaat aataggcagc
    53941 acagagggcg acgtcgagag ctgtcctgcg cgttgccccg catttttact tttttatggc
    54001 tatttttttg ccctctgttt gatcaaaaca ttcattacgc tgatgtgggg gacacaaaag
    54061 cgaaaatgca gaagaaagcc atttgctaaa attgaaggat tactactggg cgcgcagcaa
    54121 tttcgtgcgc ccctcatttg cgcaatgtaa gggtgtcata tgattactat tgacggtaat
    54181 ggcgcggttg cttcggtggc gtttcgcacc agtgaagtta tcgccatcta ccctattacc
    54241 cccagttcca cgatggcaga acaggctgat gcctgggccg gaaacggctt aaagaacgtt
    54301 tggggagaca caccacgcgt agttgaaatg cagtcggaag cgggtgctat cgctaccgtg
    54361 catggcgctt tgcagacggg tgccctttca acatcgttta cgtcatcgca gggtttgctg
    54421 ctgatgatcc cgacgctgta caaactggca ggcgaactga cgccatttgt cctgcatgta
    54481 gcggcacgta ccgttgccac acatgcactt tctatttttg gcgatcattc cgacgttatg
    54541 gcggtgcgcc agacgggttg cgcgatgttg tgtgcggcta acgtccagga agcgcaagat
    54601 tttgctctca tttcgcacat cgcaacgctg aagagccgcg tgccatttat tcatttcttt
    54661 gatggtttcc gcacgtccca cgaaatcaat aaaattgtcc cgctggccga tgacactatt
    54721 cttgatctca tgccgcaggc cgaaattgat gctcatcgcg cccgggcgct caacccggaa
    54781 catccggtga tccgcggtac gtccgccaat cctgacactt atttccagtc tcgcgaagcc
    54841 accaacccat ggtacgacgc ggtctatgac catgttgaac aggcgatgaa tgatttctct
    54901 gccgcgacag gtcgtcagta tcagccgttt gaatattacg ggcatccgca agcggaacgg
    54961 gtgattatcc tgatgggctc tgccattggc acctgtgaag aagtggttga tgaattgcta
    55021 acccgtggcg aaaaagttgg cgtgctgaaa gttcgcctgt accgtccctt ctccgccaaa
    55081 cacttgctgc aagctctgcc gggatccgta cgcagcgtgg cggtactgga cagaaccaaa
    55141 gaacccggtg cccaggcaga accgctctat ctggatgtaa tgaccgcact ggcagaagcc
    55201 tttaataatg gcgagcgcga aactctgccc cgtgtcattg gtgggcgcta tggtctttca
    55261 tccaaagaat ttggcccgga ctgtgtactg gcggtatttg ccgagctcaa cgcggctaaa
    55321 ccgaaagcgc gctttacggt tggtatttac gatgatgtga ccaatctgtc actgccgttg
    55381 ccggaaaaca ccctgccaaa ctcggcgaaa ctggaagcct tgttttatgg ccttggtagt
    55441 gatggcagcg tttccgcgac caaaaacaat atcaagatta tcggtaattc cacgccgtgg
    55501 tacgcacagg gctattttgt ttacgactcc aaaaaggcgg gcggcctgac ggtttctcac
    55561 cttcgcgtga gcgaacagcc gattcgttcc gcttatctca tttcccaggc tgattttgtt
    55621 ggctgccacc agttgcagtt tatcgataaa tatcagatgg ctgagcgttt aaaacctggc
    55681 ggcattttcc tgctcaacac gccgtacagc gcagatgaag tgtggtcgcg cttgccgcag
    55741 gaagttcagg ccgtgctaaa tcagaaaaaa gcgcgcttct atgtgattaa cgcggcgaaa
    55801 atcgcccgcg aatgtggcct ggcggcccgt attaataccg tcatgcagat ggcttttttc
    55861 catctgacgc aaattctgcc tggcgatagc gccctcgcag aattgcaggg tgcgattgcc
    55921 aaaagttaca gtagcaaagg ccaggatctg gtggaacgca actggcaggc tctggcgctg
    55981 gcacgtgaat ccgtagaaga agttccgttg caaccggtaa atccgcacag cgccaatcga
    56041 ccgccagtgg tttccgatgc cgcccctgat ttcgtgaaaa ccgtaaccgc agcgatgctc
    56101 gcagggcttg gtgatgccct ccccgtttcg gcgctgccgc cagacggcac ctggccgatg
    56161 ggcaccactc gctgggaaaa acgcaatatc gccgaagaga tccccatctg gaaagaggaa
    56221 ctctgtaccc aatgtaacca ctgcgttgcc gcttgcccac actcagctat tcgcgcaaaa
    56281 gtggtgccgc ctgaagcgat ggaaaacgcc cctgccagcc tgcattcgct ggatgtgaaa
    56341 tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg caccggaaga ttgcaccggt
    56401 tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc agaatccaga gattaaagcc
    56461 atcaatatga tgtctcgcct ggaacatgtc gaagaagaga aaatcaatta cgatttcttc
    56521 ctcaacctgc cagaaatcga ccgtagcaaa ctggaacgta ttgatattcg tacatcgcag
    56581 ctgattacac cgctgtttga atattcaggt gcttgctccg gttgtggcga gacgccgtat
    56641 attaaattgc tgactcagct ctatggcgac cggatgttga tcgctaacgc caccggctgt
    56701 tcttcaattt atggcggtaa cctgccctct acaccgtata ccaccgatgc caacggtcgt
    56761 gggccggcat gggcgaactc tctatttgaa gataatgccg aatttggcct tggtttccgc
    56821 ctgacggtcg atcaacaccg tgtccgcgtg ctgcgtctgc tggatcaatt tgccgataaa
    56881 atcccggcgg aattactgac ggcgttgaaa tcagacgcca cgccagaggt tcgtcgtgaa
    56941 caggttgcag ctttacgcca gcaactcaac gatgttgccg aagcacatga actgctacgt
    57001 gatgcagatg cactggtgga aaaatcaatc tggctgattg gtggtgatgg ctgggcttac
    57061 gatatcggct ttggcggtct ggatcatgta ttgagtttga cggaaaacgt caacattctg
    57121 gtgctggata cgcaatgcta ttccaacacc ggtggtcagg catcgaaagc gacaccgctg
    57181 ggtgcagtaa ctaaatttgg cgagcacggc aaacgtaaag cgcgtaaaga tcttggcgtc
    57241 agtatgatga tgtacggtca tgtttatgtg gcgcagattt ctctcggcgc gcagctgaac
    57301 cagacggtga aagcgattca ggaagcggaa gcgtatccgg ggccatcgct gatcattgct
    57361 tatagcccgt gtgaagagca tggttacgat ctggcactca gccacgacca gatgcgccaa
    57421 ctcacagcta ccggcttctg gccgctatat cgctatgatc cgcgtcgtgc cgatgaaggc
    57481 aaactgccgc tggccttgga ttcacgcccg ccgtcagaag cactggaaga aacgttactt
    57541 cacgagcaac gtttccgtcg gctgaattcg cagcagccag aagtggcaga acagttatgg
    57601 aaagatgctg cagctgattt gcaaaaacgc tatgacttcc tggcacaaat ggccggaaaa
    57661 gcggaaaaaa gcaacaccga ttaaatgctc tggataagga ttatccaatt ctaaaaaaaa
    57721 agcccggacg actgttcggg cttgtctttt tatatgttgg aaaatcagtg gcaatgcaat
    57781 ggcccaacag aaaataaaat ctgtgacaat aaaggcatat aacccgcgca gaataacgta
    57841 tagcaaatta aaagtata
//
LOCUS       DAZFRN010000008        55986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFRN010000008 DAZFRN010000000
VERSION     DAZFRN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.008"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..241
                     /locus_tag="ACHKUB_000480"
     CDS             <1..241
                     /locus_tag="ACHKUB_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653553.1"
                     /translation="AGIPLRAELLEMVYNTADQLSVAAEAERFDFNKSRIHPDIYMNE
                     ILTGMRIIHQVLPVIMQKLEITDFKLDETDLCLGK"
     gene            complement(316..402)
                     /locus_tag="ACHKUB_000481"
     tRNA            complement(316..402)
                     /locus_tag="ACHKUB_000481"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(366..368),aa:Leu,seq:gag)
     gene            complement(417..749)
                     /gene="secG"
                     /locus_tag="ACHKUB_000482"
     CDS             complement(417..749)
                     /gene="secG"
                     /locus_tag="ACHKUB_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO6653554.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(977..2314)
                     /gene="glmM"
                     /locus_tag="ACHKUB_000483"
     CDS             complement(977..2314)
                     /gene="glmM"
                     /locus_tag="ACHKUB_000483"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO6653555.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2307..3155)
                     /gene="folP"
                     /locus_tag="ACHKUB_000484"
     CDS             complement(2307..3155)
                     /gene="folP"
                     /locus_tag="ACHKUB_000484"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO6653556.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3245..5179)
                     /gene="ftsH"
                     /locus_tag="ACHKUB_000485"
     CDS             complement(3245..5179)
                     /gene="ftsH"
                     /locus_tag="ACHKUB_000485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO6653557.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5279..5908)
                     /gene="rlmE"
                     /locus_tag="ACHKUB_000486"
     CDS             complement(5279..5908)
                     /gene="rlmE"
                     /locus_tag="ACHKUB_000486"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO6653558.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6034..6327
                     /gene="yhbY"
                     /locus_tag="ACHKUB_000487"
     CDS             6034..6327
                     /gene="yhbY"
                     /locus_tag="ACHKUB_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO6653559.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6483..6959)
                     /gene="greA"
                     /locus_tag="ACHKUB_000488"
     CDS             complement(6483..6959)
                     /gene="greA"
                     /locus_tag="ACHKUB_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO6653560.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7207..8640
                     /gene="dacB"
                     /locus_tag="ACHKUB_000489"
     CDS             7207..8640
                     /gene="dacB"
                     /locus_tag="ACHKUB_000489"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6653561.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8680..9852)
                     /gene="cgtA"
                     /locus_tag="ACHKUB_000490"
     CDS             complement(8680..9852)
                     /gene="cgtA"
                     /locus_tag="ACHKUB_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO6653562.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9868..10833)
                     /gene="yhbE"
                     /locus_tag="ACHKUB_000491"
     CDS             complement(9868..10833)
                     /gene="yhbE"
                     /locus_tag="ACHKUB_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6653563.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(10960..11217)
                     /gene="rpmA"
                     /locus_tag="ACHKUB_000492"
     CDS             complement(10960..11217)
                     /gene="rpmA"
                     /locus_tag="ACHKUB_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO6653564.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11238..11549)
                     /gene="rplU"
                     /locus_tag="ACHKUB_000493"
     CDS             complement(11238..11549)
                     /gene="rplU"
                     /locus_tag="ACHKUB_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO6653565.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11808..12779
                     /gene="ispB"
                     /locus_tag="ACHKUB_000494"
     CDS             11808..12779
                     /gene="ispB"
                     /locus_tag="ACHKUB_000494"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO6653566.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13007..13285
                     /gene="sfsB"
                     /locus_tag="ACHKUB_000495"
     CDS             13007..13285
                     /gene="sfsB"
                     /locus_tag="ACHKUB_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO6653567.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13333..14592)
                     /gene="murA"
                     /locus_tag="ACHKUB_000496"
     CDS             complement(13333..14592)
                     /gene="murA"
                     /locus_tag="ACHKUB_000496"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO6653568.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14647..14901)
                     /gene="ibaG"
                     /locus_tag="ACHKUB_000497"
     CDS             complement(14647..14901)
                     /gene="ibaG"
                     /locus_tag="ACHKUB_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO6653569.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15061..15354)
                     /gene="mlaB"
                     /locus_tag="ACHKUB_000498"
     CDS             complement(15061..15354)
                     /gene="mlaB"
                     /locus_tag="ACHKUB_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO6653570.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15354..15989)
                     /gene="mlaC"
                     /locus_tag="ACHKUB_000499"
     CDS             complement(15354..15989)
                     /gene="mlaC"
                     /locus_tag="ACHKUB_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO6653571.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16008..16559)
                     /gene="mlaD"
                     /locus_tag="ACHKUB_000500"
     CDS             complement(16008..16559)
                     /gene="mlaD"
                     /locus_tag="ACHKUB_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO6653572.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16564..17346)
                     /gene="mlaE"
                     /locus_tag="ACHKUB_000501"
     CDS             complement(16564..17346)
                     /gene="mlaE"
                     /locus_tag="ACHKUB_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO6653573.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17354..18163)
                     /gene="mlaF"
                     /locus_tag="ACHKUB_000502"
     CDS             complement(17354..18163)
                     /gene="mlaF"
                     /locus_tag="ACHKUB_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO6653574.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18373..19350
                     /gene="yrbG"
                     /locus_tag="ACHKUB_000503"
     CDS             18373..19350
                     /gene="yrbG"
                     /locus_tag="ACHKUB_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO6653575.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19364..20350
                     /gene="kdsD"
                     /locus_tag="ACHKUB_000504"
     CDS             19364..20350
                     /gene="kdsD"
                     /locus_tag="ACHKUB_000504"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO6653576.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20371..20937
                     /gene="kdsC"
                     /locus_tag="ACHKUB_000505"
     CDS             20371..20937
                     /gene="kdsC"
                     /locus_tag="ACHKUB_000505"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO6653577.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            20934..21509
                     /gene="lptC"
                     /locus_tag="ACHKUB_000506"
     CDS             20934..21509
                     /gene="lptC"
                     /locus_tag="ACHKUB_000506"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO6653578.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPIYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21478..22035
                     /gene="lptA"
                     /locus_tag="ACHKUB_000507"
     CDS             21478..22035
                     /gene="lptA"
                     /locus_tag="ACHKUB_000507"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO6653579.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22042..22767
                     /gene="lptB"
                     /locus_tag="ACHKUB_000508"
     CDS             22042..22767
                     /gene="lptB"
                     /locus_tag="ACHKUB_000508"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO6653580.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22815..24248
                     /gene="rpoN"
                     /locus_tag="ACHKUB_000509"
     CDS             22815..24248
                     /gene="rpoN"
                     /locus_tag="ACHKUB_000509"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO6653581.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24271..24558
                     /gene="hpf"
                     /locus_tag="ACHKUB_000510"
     CDS             24271..24558
                     /gene="hpf"
                     /locus_tag="ACHKUB_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO6653582.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24676..25167
                     /gene="ptsN"
                     /locus_tag="ACHKUB_000511"
     CDS             24676..25167
                     /gene="ptsN"
                     /locus_tag="ACHKUB_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO6653583.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25213..26067
                     /gene="rapZ"
                     /locus_tag="ACHKUB_000512"
     CDS             25213..26067
                     /gene="rapZ"
                     /locus_tag="ACHKUB_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO6653584.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26064..26336
                     /gene="npr"
                     /locus_tag="ACHKUB_000513"
     CDS             26064..26336
                     /gene="npr"
                     /locus_tag="ACHKUB_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO6653585.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26550..27182
                     /gene="yrbL"
                     /locus_tag="ACHKUB_000514"
     CDS             26550..27182
                     /gene="yrbL"
                     /locus_tag="ACHKUB_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO6653586.1"
                     /translation="MIRLSEQNPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27179..27907)
                     /gene="mtgA"
                     /locus_tag="ACHKUB_000515"
     CDS             complement(27179..27907)
                     /gene="mtgA"
                     /locus_tag="ACHKUB_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO6653587.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27904..28557)
                     /gene="elbB"
                     /locus_tag="ACHKUB_000516"
     CDS             complement(27904..28557)
                     /gene="elbB"
                     /locus_tag="ACHKUB_000516"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO6653588.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28787..31123)
                     /gene="arcB"
                     /locus_tag="ACHKUB_000517"
     CDS             complement(28787..31123)
                     /gene="arcB"
                     /locus_tag="ACHKUB_000517"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO6653589.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31219..32148)
                     /gene="yhcC"
                     /locus_tag="ACHKUB_000518"
     CDS             complement(31219..32148)
                     /gene="yhcC"
                     /locus_tag="ACHKUB_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO6653590.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            32823..37283
                     /gene="gltB"
                     /locus_tag="ACHKUB_000519"
     CDS             32823..37283
                     /gene="gltB"
                     /locus_tag="ACHKUB_000519"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO6653591.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMIFLNKDSELAAAARRTV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37296..38714
                     /gene="gltD"
                     /locus_tag="ACHKUB_000520"
     CDS             37296..38714
                     /gene="gltD"
                     /locus_tag="ACHKUB_000520"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO6653592.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMHGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            38898..39203
                     /locus_tag="ACHKUB_000521"
                     /pseudo
     CDS             38898..39203
                     /locus_tag="ACHKUB_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            39314..39619
                     /locus_tag="ACHKUB_000522"
     CDS             39314..39619
                     /locus_tag="ACHKUB_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6653593.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(39695..40159)
                     /gene="nanQ"
                     /locus_tag="ACHKUB_000523"
     CDS             complement(39695..40159)
                     /gene="nanQ"
                     /locus_tag="ACHKUB_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO6653594.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40156..41031)
                     /gene="nanK"
                     /locus_tag="ACHKUB_000524"
     CDS             complement(40156..41031)
                     /gene="nanK"
                     /locus_tag="ACHKUB_000524"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO6653595.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHLSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41028..41717)
                     /gene="nanE"
                     /locus_tag="ACHKUB_000525"
     CDS             complement(41028..41717)
                     /gene="nanE"
                     /locus_tag="ACHKUB_000525"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO6653596.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(41765..43255)
                     /gene="nanT"
                     /locus_tag="ACHKUB_000526"
     CDS             complement(41765..43255)
                     /gene="nanT"
                     /locus_tag="ACHKUB_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO6653597.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(43364..44257)
                     /gene="nanA"
                     /locus_tag="ACHKUB_000527"
     CDS             complement(43364..44257)
                     /gene="nanA"
                     /locus_tag="ACHKUB_000527"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO6653598.1"
                     /translation="MATNLRGVMAALLTPYDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGQIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44379..45170)
                     /gene="nanR"
                     /locus_tag="ACHKUB_000528"
     CDS             complement(44379..45170)
                     /gene="nanR"
                     /locus_tag="ACHKUB_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO6653599.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            45550..46917
                     /gene="dcuC"
                     /locus_tag="ACHKUB_000529"
     CDS             45550..46917
                     /gene="dcuC"
                     /locus_tag="ACHKUB_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO6653600.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLDGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(46960..47457)
                     /gene="sspB"
                     /locus_tag="ACHKUB_000530"
     CDS             complement(46960..47457)
                     /gene="sspB"
                     /locus_tag="ACHKUB_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO6653601.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(47463..48101)
                     /gene="sspA"
                     /locus_tag="ACHKUB_000531"
     CDS             complement(47463..48101)
                     /gene="sspA"
                     /locus_tag="ACHKUB_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO6653602.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(48496..48888)
                     /gene="rpsI"
                     /locus_tag="ACHKUB_000532"
     CDS             complement(48496..48888)
                     /gene="rpsI"
                     /locus_tag="ACHKUB_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO6653603.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(48904..49332)
                     /gene="rplM"
                     /locus_tag="ACHKUB_000533"
     CDS             complement(48904..49332)
                     /gene="rplM"
                     /locus_tag="ACHKUB_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO6653604.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(49551..50678)
                     /gene="zapE"
                     /locus_tag="ACHKUB_000534"
     CDS             complement(49551..50678)
                     /gene="zapE"
                     /locus_tag="ACHKUB_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO6653605.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMQRMHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            50872..51270
                     /gene="zapG"
                     /locus_tag="ACHKUB_000535"
     CDS             50872..51270
                     /gene="zapG"
                     /locus_tag="ACHKUB_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO6653606.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            51424..52791
                     /gene="degQ"
                     /locus_tag="ACHKUB_000536"
     CDS             51424..52791
                     /gene="degQ"
                     /locus_tag="ACHKUB_000536"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO6653607.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            52881..53948
                     /gene="degS"
                     /locus_tag="ACHKUB_000537"
     CDS             52881..53948
                     /gene="degS"
                     /locus_tag="ACHKUB_000537"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO6653608.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(54011..54949)
                     /gene="mdh"
                     /locus_tag="ACHKUB_000538"
     CDS             complement(54011..54949)
                     /gene="mdh"
                     /locus_tag="ACHKUB_000538"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6653609.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            55384..55854
                     /gene="argR"
                     /locus_tag="ACHKUB_000539"
     CDS             55384..55854
                     /gene="argR"
                     /locus_tag="ACHKUB_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO6653610.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        13774 a        15018 c        13937 g        13257 t
ORIGIN      
        1 ggcgggaatt ccgttaagag ccgaacttct ggaaatggtt tataacaccg ctgaccaact
       61 tagcgttgca gcagaagccg aacgtttcga tttcaataaa tctcgcattc atccagatat
      121 atacatgaac gagatcctta ctggcatgag aataattcac caagtgcttc cggtaataat
      181 gcaaaagtta gaaattacag atttcaaact agatgaaaca gatttatgcc tgggtaaata
      241 gttatcaaat taagaatact tagcctggct tataaaacga aaaaagacgc ttttcagcgt
      301 ctcttttctg gaatttggta ccgaggacgg gacttgaacc cgtaagccct attgggcact
      361 accacctcaa ggtagcgtgt ctaccaattc caccacctcg gcacggatac tactttttag
      421 ttcgggatat cgctggtcgg cttagccgga gcagctggtt gagtttgttc ggttttcacc
      481 ggtgcactca gattttccca ttcgctacct ttattggttt tgttgctatt gatgttaccc
      541 agcaccagac tgatgatgaa gaataacgtt gccagcagcg ccgtcatgcg ggtcatgaag
      601 ttaccagaac cacttgaacc aaacagcgta gcggaagcgc ctgctccgaa ggaggctccc
      661 atatcagcgc ctttaccttg ctgcagcatg atcagaccaa caaggccaat tgccacaata
      721 aggaaaacta ctaaaagagc ttcgtacata atcaacctgt tccttgcgga gttgccgcgt
      781 accaatgctt caaccaataa agcgggagtt tttaatcttt cccactgaag cgggtgtgaa
      841 tactaaccaa agcgaatgac cttcgcaagg gcaattttat cgcattgtat caactgcgga
      901 aaaaaacagc aaaaccgatg tgttcgctga gaaaaaaggc gacaatcgcc gcctttttag
      961 ccagttgtct aacgctttaa acggctttta ctgcatcggc gatgcggtgt gcaaattcag
     1021 tcacctgcgc ttcgtcttcg ccttccacca tcacgcgaat taacggttcg gtgccggatt
     1081 tacgcagcaa cacgcgtcca cggttgccca gcgcagcttc aacctctgcg gtcacggctt
     1141 taactgactc atgctcaagt ggatcgccgc tacctgcggt gtaacgtacg ttaaccagaa
     1201 tctgcgggaa cattttcatg ccgctgcaaa ggtcgtgcag gctcatatgg ttacgtgcca
     1261 tcgccgccag cacctgcaag ccagcaacga tgccgtcacc ggtagtagtt ttatccagta
     1321 ggatcacatg accggaattc tctgcaccga tacgccagcc tttctcctgc attttttcca
     1381 gtacgtagcg gtcacccact ttcgcgcgcg caaatggaat tcctaactgt ttcagcgcca
     1441 gttcaagccc catgttgctc atcaatgtac ccacagcgcc accacgcagc tggccctgac
     1501 gaagaccttc acgcgcgatg atatacatga tctgatcgcc atcgacttta ttgccttcat
     1561 ggtcgaccat aatcacgcga tcgccatcgc cgtcgaaggc aataccgaga tccgcttttt
     1621 cagccagcac acgagcctgg agcgcgcgaa cgtcggtagc ccccacttcg gcattgatgt
     1681 ttacaccgtt tggctcacaa ccgatagcga taacgttcgc ccccagttcg cgcagcacgt
     1741 tcggcgcgat gtgataagtc gcaccgtttg cacaatccac cacaatcttc agttcactga
     1801 ggctaagttc gttcgggaac gtggctttgc aaaactcgat atagcgaccc gcggcatcaa
     1861 cgatacggct ggctttaccc agttctgccg aatcaacgca gctgatctcc ttttccattt
     1921 ccgcttcgat ggcctcttct accgcatccg gcagtttggt gccgtcgata gagaagaatt
     1981 taatcccatt atcgtagaac gggttatgcg atgcagatat cacaattccg gcctctgcgc
     2041 ggaaggtacg cgtcagataa gccacggccg gtgttggcat cgggccagtg aagagtgcgg
     2101 aaaggcccgc tgccgccaga cccgcttcca gtgctgactc cagcatatag ccagaaatac
     2161 gcgtgtcttt accaataata atcttacggg agccgtggcg cgccagcact ttacccgcgg
     2221 cccaacccag cttaagcaca aaatcaggtg tgatcggcgc atcccctaca cgaccacgaa
     2281 tcccatcggt accgaaatat ttacgattac tcatagcgtt tgttttcctt tgcagacaga
     2341 gtggcttcca ccacccgcat cgcttctacg gtttctttga cgtcatgaac acgaatgatg
     2401 tgcgcgcctt gcattgcggc aatgaccgca caggccagac taccgctcag gcgctcggac
     2461 ggccccacgt tcagcagctg cccaatcatc gattttcgtg acatacccac caacagcggc
     2521 aggttgaaat ggtgaaattc agccaggcgc gccagtaatg aatagttatg ggagagattt
     2581 ttaccgaaac cgaatccggg gtcgagcaac aatttctctt ttgcgatacc cgcctgctcg
     2641 caacgtgcta tttgctcaat aaagtagcga ttcacttctg caaagacatc gtcatacttc
     2701 ggagcttcct gcatggtttt tggatttccc tgcatatgca tcagacaaac cggtaaaccg
     2761 gtttctgcag ccgcctccag agcgccaggt tcggaaaggg agcggatatc attaataatg
     2821 tgagcgccaa ctttcgctga ctcacggatg acttctggtt tggatgtatc gaccgagatc
     2881 cagacttcga agcgttgagc aattgcctca accacaggaa taacacgttg caactcttct
     2941 tcaacgctaa cttccgccgc ccctgggcgc gtggactcgc caccaacgtc aatgatcgtc
     3001 gcgccagcat tgatcatcag attcgcatgt ttcaccgcat ctatcagcga gttatgcgtg
     3061 ccaccatccg aaaaggaatc aggcgtgacg ttgaggatcc ccattacgtg aggatggcta
     3121 aggtccagtg aagtaccctg ggcaaagagt ttcatgatgt tatccctggt atgaattgat
     3181 aagaaaaaac cccggagcac gccccggggt tttcggtaca aatacagtca tctgatgcgg
     3241 gaacttactt gtcgcctaac tgctctgaca tggtgttacc cgggttcggc gtacgcggtt
     3301 catcaaccgg acgaggagcc tttggactac cattgtcgcc agaattgtta gaagcgcctg
     3361 gttcttccca gcccgctggc ggacgtacat cgcgacgtgc catcaggtca tcaatctgcg
     3421 gtgcgtcgat agtctcatat ttcatgagag catctttcat cgcatgcaga atatccatat
     3481 tgtcggtcag aagctgacgc gcacgattat agttacgctc aatcagtgct ttcacttcct
     3541 ggtcgatgat acgtgcagtt tcatcggaca tatgtttcgc tttcgctacg ctacggccga
     3601 ggaatacttc accttcttct tccgcgtaca gcagtggacc caatttctca gagaagcccc
     3661 actgggtcac catgttacgt gccagattgg tcgcaacttt aatatcgttg gacgcaccgg
     3721 tagaaacatg ttccggccca tagatgatct cttctgccag acgaccaccg tacagcgtag
     3781 aaatctggct ttccagtttc tgacggctgg cgctgattgc gtcgccctca ggcaagaaga
     3841 aagtcacacc cagcgcacga ccgcgtggga taatcgtcac tttgtgcacc ggatcgtgtt
     3901 ccggcaccag gcgaccgata atcgcatgac ccgcttcgtg gtaagccgtt gattctttct
     3961 gcgcttccgt catcaccatg gagcgacgtt ccgcacccat catgattttg tctttcgctt
     4021 tctcgaactc aaccatcgac acaacgcgtt tgttgccacg agcagcgaac agtgccgctt
     4081 cgttcaccag gttcgccagg tcagcaccgg agaaaccagg agtaccacgg gcaatgattg
     4141 ccgcgtcgat atcgggggcc aatggtacgc gacgcatgtg aactttcagg atctgctcac
     4201 gaccgcgaac atctggcaag ccgaccacaa cctgacggtc gaaacggcca ggacgcagca
     4261 gggccgggtc gagaacgtcc ggacggttag tcgcggcgat aacgatgata ccttcgttac
     4321 cttcgaagcc atccatctca accagcatct ggttcagagt ctgttcacgt tcatcgtgac
     4381 caccgcccag accagcgcca cgctggcggc ctacggcgtc gatttcatcg ataaagatga
     4441 tgcacggtgc cgctttcttc gcctgttcga acatgtcacg aacacgggat gcacccacac
     4501 cgacgaacat ttctacgaag tcagaaccgg agatagtaaa gaacggaact ttcgcttcgc
     4561 ctgcaatcgc tttcgccagc agcgttttac cggtacccgg aggaccgacc atcaggacgc
     4621 ctttcgggat cttaccgccg agtttctgga agcggctcgg ctcgcgcaga tactctacca
     4681 gttcagcaac ttcttctttt gcttcgtcgc agcccgcaac gtcagcaaag gtcgttttga
     4741 tctgatcttc cgtcagcatg cgcgctttgc tcttaccaaa cgacatggca cctttgccac
     4801 cgccgccctg catttgacgc atgaagaaga tccagacacc aatcagcaac agcatcggga
     4861 accaggagat gaagatagaa gccagcaggc ttggttcttc aggcggttca ccgacaacct
     4921 tgacgttctt ggtcaacagg ttatccagta atttcggatc ctgaaccgga atgtaagtgg
     4981 tataacggtt actatctttc ttggtaacgt tgatttcacg tccgttgata cgcgcttcac
     5041 gaacctggtc gttattgacc tcttgtagga aggtagagta atccacctta cggccattag
     5101 actcgctggg cccaaagctc tggaatactg acatcagcac aacggcaatg accagccaga
     5161 gtattaggtt tttcgccatg tcactcaagg gattaacctc ttattacaac tgtgttaaaa
     5221 acagcgtcag gatactctat atccagcatc tttcaaactt tcgtctgaaa tctcccggtt
     5281 agggtttacg cccggtcgct acaatataca cttcccgcga acgtgcacga gaagagtccg
     5341 gcttacgaac tttgaccttc gtaaacaggg agcgaatttc ccttagatac tcatcgaaac
     5401 cttcgccctg gaacaccttc actacaaaac tgccacctgg cgctaataca tcacgacaca
     5461 tttctagcgc cagttccacc agatacatgg cacgggggat atccaccgcc ggtgttccgc
     5521 tcatgtttgg tgccatatcg gacatgacaa cctggacttt gctgtcgcca acgcgctcca
     5581 gcagtgcttt catcaccagt tcatcacgaa aatcgccctg aagaaagtcc acaccaacga
     5641 taggatccat aggtaaaaga tcgcaagcga tgatgcggcc tttgccgcca atttgggtga
     5701 ccacatattg tgaccaacca cccggagcag caccaaggtc gacaaccgtc attcccggtt
     5761 taaagagttt gtcactttgc tgtatttcat caagtttaaa ccaggcacgg gaacgtaacc
     5821 cctttttctg tgcctgttga acatatttat cgctaaagtg ttcctgaagc cagcggctgg
     5881 agctggcaga acgcttctta cctgtcattt aactttccca tggggataac tcatcgtaac
     5941 caattgcgta aatttttacg cgcctatttg gtgatatatg ggagatggcg gtagaatgac
     6001 ccgttttcaa tcccaacgta agcaaaaaat acgatgaatc tgagtactaa acaaaaacag
     6061 cacctgaaag gtctggcaca tccgctcaag ccagttgttc tgcttggcag taatggtttg
     6121 accgaagggg tgctggccga gattgaacaa gcgttagagc accatgaact catcaaggtg
     6181 aaaatcgcca ccgaagatcg cgaaactaaa accttgatcg tggaagctat cgtgcgcgaa
     6241 accggcgcct gtaatgtaca ggtcatcggt aaaacgctgg tgctttatcg cccaactaaa
     6301 gaacgtaaaa tctcgctgcc acgctaagat tatcctaaag ttacacacat ttgctgtgta
     6361 aaacgagggg ttttccgcag gcaggagagc aaaatgccac gctctgttcg ttgataaaag
     6421 gccgcatagc ggcctttttc ctttctttac aatacatcaa catcttgagt attgggtaat
     6481 tcttacaggt attccacctt aattacttca aattctactt cgccgcccgg cgttttgatg
     6541 accacaacat catcttcttc tttgccgatc aggccacgag caataggcga gtttacagaa
     6601 atcaggtttt gtttaaagtc agcttcgtca tcgccaacga tgcgataagt ctgttcttcg
     6661 tcagaatcca gattcagcac cgttacggta gcaccaaaaa taacgcgccc attgttgggc
     6721 attttggtga catcaatcac ctgcgcgttc gacagcttgg cttcgatgtc tttaatacgg
     6781 ccttcgcaga aaccctgctg ttcacgagct gcgtggtatt cggcgttttc tttcaggtcg
     6841 ccatgctcac gcgcttccgc gatagcagca atgatttcag gacggcgcac agatttcaga
     6901 aaatccagct cttcgcgtaa tttttcagcg ccgcgtaagg tcatcggaat agcttgcatt
     6961 tgttatacct cttgaatatt cctgatgggg caagtcttca cccaccccgg ctgttcggcc
     7021 tgcccggcat aactcctcac cgggaccaga agcaaaaaaa taccgacccg ggtacaagtc
     7081 ccaggtcagc tacaattcac attttgatag tcattttacc ctgaagttcc cgaagggtca
     7141 tcgtttactt tatagggcgt tgcgccgtag tatgacggct cgattccagg ttgttagcgc
     7201 gagattatgc gattttccag atttatcatc ggattgacca gctgtatagc gttcagtgtt
     7261 caggccgcga atgttgatga gtacgttact caactccccg ctggtgccaa ccttgccctg
     7321 atggtgcaaa aagtcggcgc gtcggccccc gctattgatt accacagtca gcagatggcg
     7381 ctgcccgcca gtacgcagaa agtgattact gcgctggcgg cgttgattca actcggcccc
     7441 gattttcgtt tcaccacgac gcttgaaacc aaaggcaatg tggaaaacgg cgtacttaag
     7501 ggtgacttag tggcgcgatt tggtgccgat ccgacgttaa aacgtcagga tattcgcaat
     7561 atggtcgcga ctttgaaaaa atctggcgtc aaccaaatcg atggcaatgt gttgatagat
     7621 acctccattt tcgccagcca cgataaagcc cccggctggc catggaatga catgacacaa
     7681 tgctttagcg ctccgcctgc cgctgccata gttgaccgca actgtttctc cgtctcgctc
     7741 tacagtgccc caaaacctgg tgatatggct tttatacgcg tggcatctta ttaccccgtt
     7801 acgatgttca gccaggtacg caccctcccc cgaggttctg ccgaagcgca atactgcgaa
     7861 ctggatgtcg taccaggcga cctgaaccgc tttacgctga cgggatgcct gccacaacgt
     7921 tctgagccgc tcccgttggc ttttgccgtg caggatggag ccagctatgc cggggcaatt
     7981 ctgaaagatg agttaaaaca ggcaggtatc acctggagcg gaacactgct gcgccagact
     8041 caggttaacg aacctggaac ggtagttgcc agtaaacagt cggccccgtt gcacgatctg
     8101 cttaagatta tgctgaaaaa gtcggacaac atgatcgccg atacggtttt ccgcatgata
     8161 ggccatgcgc gcttcaatgt gcctggaaca tggcgggccg ggtcggacgc cgtgcgtcag
     8221 atcctgcgcc agcaagccgg tgtcgatatt ggaaacacca ttattgccga tggttcaggg
     8281 ctttcgcggc ataacctgat tgcccccgcc accatgatgc aggtgttgca atacattgcc
     8341 caacacgaca atgaacttaa ctttatctcc atgctgccgc tggcgggcta tgacggctct
     8401 ttgcagtacc gtgcaggtct gcatcaggcg ggcgtggatg gaaaagtctc agcgaaaacc
     8461 ggttcgttgc agggggtata taacctggcg ggattcatta ccacagcgag cgggcaacga
     8521 atggcgtttg tacaatatct ttctggctat gcagtagaac cggcggatca gcgtaatcgc
     8581 cgtattccgt tagtgcgttt tgaaagccgt ttgtataaag atatttatca gaacaattag
     8641 tcaaaaagaa accccggcac atggccgggg ttttgctgat taacgcttgt aaatgaactc
     8701 aacgccttct tcgtcgtctt cgtcccagtc atcatcccag tcctcatcat cctcttcagc
     8761 aatctcttca agctgctggc gatgataatc atcccacatg aattcgactt tctctggctg
     8821 tttcgcttct tcagcctgca cgaccgggtt ttcaatgata aaggtcatca catcccagca
     8881 gagatctttc acgcccagtc cactcgccgc agagatcaga taatatttat cttcccagcc
     8941 cagcgcttca gcaatcgctt tcgctttctc ttcagcttct gccttatcca gcagatcgat
     9001 cttattgaac actaaccaac gtggtttcgc cgccagatcc tggctgtatt tttccagctc
     9061 gctgataata atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat cgatatcgat
     9121 gaggtgcaac aggacgcggc aacgttccag gtgcttcagg aagcgaatgc ccagacctgc
     9181 gccttccgca gcgccttcaa tcagtcctgg aatatcggca acaacgaagc tcttttcgtt
     9241 gtccattcgt accacaccca gacttggcac cagagtggta aacggataat ccgccacttt
     9301 cggtttagcc gccgataccg cacgaataaa ggtcgattta cccgcgtttg gcatccccaa
     9361 cataccgacg tcagccagca gcatcagctc cagcagcagc tcacgcttat cgcccggcgt
     9421 accgttagtt ttctgccgtg gtgtacggtt aactgacgat ttgaaacggg tattgcccag
     9481 accgtgccag ccgcccttag caaccagcag acgctgaccg tgtttggtca tatcgcccat
     9541 ggtttcacca gtaccctggt cgattacacg cgtacctacc ggcactttaa ttgtgacgtc
     9601 tttaccgcgc ttaccggtac agtcgcggct tgcgccattc tgaccgcgct ctgcacggaa
     9661 agatttttca aaacgataat cgataagcgt gttcaggttc tcgtcggctt ccatccatac
     9721 gtcaccacca tcaccgccgt cgccgccatc cgggccgcct ttcggaatat acttttcgcg
     9781 gcggaagctc acgcaaccat taccgccatc acctgcaacg accagaatcg atgcttcatc
     9841 aacaaacttc attttattct ccgtaaatca ttcgcctgcg cggggttgcg ataccaccgt
     9901 tgtatgctta cgtaatccgc cccaaatacg atgaccaatg gcggaataca tcgcgcccgc
     9961 aaccacgaca aacgcaccga gataacctaa aaggtttaac atcggtctgg cgaagaaatc
    10021 gggccaggcc agtgataaaa gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat
    10081 gatcgcgctc acctgcgctg cctgccagcg agccatcgct tccgccaggg cgccatatcc
    10141 taccaaggta ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc taagctgcgc
    10201 tatcactcca ggctttgcca gagggaagag cgcaattgta cataaagtgt acagtaaaaa
    10261 caggatctgc ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc cataactcac
    10321 ccaaaccgtc gccgcaccga ccccaaagat aactccccag gtgtaatcgg tgagctttgt
    10381 aaatatctcg accagactgg tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc
    10441 ccctacaacc tgagtgctgc gcattttctc tttcaggata aatacgctgg caaccatcat
    10501 gccaactggc gagagttgcc caatcacctg cgaagcggtc ggactcaggt attgcaagga
    10561 tgagctgaac aggatgaagt tcccaaacag cccggcggtc gccactgcca acaaaatcaa
    10621 ccagcgtggc ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc
    10681 accaaggcca atactcgcca tcaagaaacg gtaaaacacg attgtcggag gttccatcac
    10741 ctccagcacc tgcttcattg cgattggcaa cgccccccag caaattgctg tggtgagcgc
    10801 caaaagaatg ccaatgcctg cctgctgctt cataccgtat tccctgcaaa gcgcatttgc
    10861 ggattaacgt cgcaccatgc gcatttttac cggttatcga atgtaaaaag ccccgcaacg
    10921 tgttgcgggg ctttcatccg ttaccgggac gcgaaaaact tattcagctt cgatgctgat
    10981 aaatttacgg tttttcgggc ctttaacttc gaatttcact ttaccgtctg ctttagcaaa
    11041 cagagtgtgg tcacgaccgc aacctacgtt agcgccagcg tggaatttgg taccacgttg
    11101 acgaacgatg atgctacccg ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg
    11161 tttagcttct gaatcgcgac cgttacgtgt ggagccgcca gcctttttat gtgccatttg
    11221 aaatctctcc tcaggtctta ggcgctgatg ccagtaattt tcacatcagt gaaccactga
    11281 cgatggccct gctgcttacg atagtgttta cgacgacgaa acttaacgat tttaactttc
    11341 tcgccacgac cgtgagcaac aacttcagct ttgattacgc cgccatcaac gaaaggaacg
    11401 ccgattttga cttcttcacc gtttgcgatc atcagcactt cagcgaactc aacagtttcg
    11461 ccagttgcga tgtccagctt ttccaggcga acggtctgac cttcgcttac tcggtgttgt
    11521 ttaccaccac tttggaaaac cgcgtacata taaaactccg cttccgcgca caccttttca
    11581 atgattcaga gtgcgctata aatattcaca atagggcgcg aatattacgc aaaacgcacg
    11641 cctttgacaa gtgctacagt caatacacga agaaaaaaaa cacaacttgt acggtaacgt
    11701 ttatctgtgc cattttttca gtacaatcac tctatattcc taaccataaa ccctaagttg
    11761 cctttgttca cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa
    11821 aaatcaatga gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc
    11881 ttaattccga cgtccagctg atcaatcagt taggctatta catcgtcagc ggcggcggta
    11941 aacgtattcg tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg
    12001 cgcatgtcac cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctgcacgacg
    12061 acgttgtgga tgaatcagat atgcgcaggg gtaaagctac cgccaacgct gcatttggca
    12121 atgccgccag cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca
    12181 gcctcggttc gctcaaagtg ctggaagtca tgtcagaagc cgtaaacgtc atcgcagaag
    12241 gtgaagttct gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc
    12301 gcgttatcta tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc
    12361 tggctggctg tacgccggaa gaggagaaag gcctgcagga ttatgggcgc tatctcggca
    12421 ctgctttcca gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta
    12481 aaaatgtcgg cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcgatgc
    12541 atcatggcac accagaacag gcacagatga tccgtaccgc catcgaacag ggtaacggtc
    12601 gccatcttct ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc
    12661 gtcagcgtgc cgaggaagaa gcagacaaag ccatcgcagc attacaggtg ctcccggaca
    12721 ccccttggcg agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat
    12781 cccctccctt catcccgcgc agtgcgcggg atgattccag taaattccat aaaaacacta
    12841 attcagcgct ataacacctg aaaaaaaggg cgatgaatct ttatatattc tgaatattca
    12901 cactctttac aggaactttt tagagcaata ggccatcagg agtatagtga tgcccgacag
    12961 aagaagtgtt ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt
    13021 cattgactgg catcccgctg acatcattgc gggtttgcgc aagaagggaa cgtcaatggc
    13081 ggcggagtct cgcagaaatg gtttgagttc ctcaacgctg gcaaacgcat tatcgcgccc
    13141 atggccgaaa ggagagatga ttattgcgaa agccctggga actgacccct gggttatctg
    13201 gccatcacgc taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg
    13261 tagctacact aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac ggggctgcca
    13321 gctctcagac gattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgcagttt
    13381 gtcttcaatg cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc
    13441 ttccgcaata cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac
    13501 ctgtgcgcca gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc
    13561 atgcgcgccc atacggctaa gctccggcac atgcataaag cggttttcaa agaccgtttc
    13621 ggtgatgaat ccggtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc
    13681 ggtcgggaat gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg
    13741 catatccagg ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt
    13801 cgccagcacg gcgtcgagag tatctggctg cgcgttacgg cagataattt tgccgcgaga
    13861 aatcgccgcc gccaccagga aagtaccggt ttcgatacga tccggtagca cgcgatagac
    13921 accgccgcct aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat
    13981 tttcgcaccc agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc
    14041 gttttcaata atcgtggtac cttccgccag ggttgcagca cacatgatgg tcaccgttgc
    14101 gccaacgctg actttatcca tcacgatatg tgcgcctttc agacgaccat cgacggaagc
    14161 tttaacgtaa ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg
    14221 tagatcaacc ggacgcgcac cgatcgtaca accgccaggc agtgaaactt gcccctgacc
    14281 aaagcgcgct accagcggcc ccagtgccca gatagaagca cgcatggttt taaccagatc
    14341 gtaaggtgcg cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc
    14401 tactttcgcc cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagttttgg
    14461 gacgttctgg atctctaccg gttcttccgc cagtagagcg gcaaaaagga taggcagagc
    14521 agcattttta gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg
    14581 aaatttatcc atttagtttg ttctcagtta acaattcata tccgctaccg gctaatcgcc
    14641 catagctcaa aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt
    14701 gatcgacaca gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt
    14761 ctgctgtttt ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa
    14821 gtggctgcca tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct
    14881 ctgaatttca ttattttcca tgggatcttc aatcatcagt taataaacca gcgaaacatc
    14941 ttagagcaaa gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg
    15001 ctcgacttgc aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat
    15061 ttaacgaggc agaacatcag caggcaaatt atacaatttc gcgagggtat acactttgtc
    15121 gtttaccccc tgaagcgtca cgttgttgcc ctgctttttc gccagatcga taagatggag
    15181 cagcagtgcc agtccacccg tatccacgcg ggacacacgg ctaagatcga tgcaggtaat
    15241 ccccttcacc gcttcctcac gcatttccca aagcggtagc agaacgtcct gatccagctc
    15301 tccggataac gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt
    15361 ttctcttcca gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg
    15421 atacctttgg tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg
    15481 ccttcagcaa tcatgtcgta agcctgccag tttcccgtct gggagttttt acgccactgg
    15541 aagtccagac gcaccggcgg acggccattc gggtcaataa tggtaacgcg aataggcaca
    15601 atggttttat cgcccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc
    15661 gccagcgcct gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaggcttca
    15721 cgttgagcag gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc
    15781 acctgtacgt atggcagcag ttcctgatca acaatggtac gcagataatc cgggttggcc
    15841 cgaatttgcg gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc
    15901 agcttatacg gattggtctg gtctgccgcg gttgccgcac tcagaggtgc aatcaccagc
    15961 aaagcgacca tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta
    16021 cccacaggtt cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc
    16081 ttattgtcat cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc
    16141 gcagacttag tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg
    16201 tcttcaaaac cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg
    16261 ctcagcgaac tggtatctgg aatgtggtta taacgttgtt caatttccag cgttacgcgc
    16321 ggcagatagg ttttcgggtc cagcgtaata tccgccaccc gacccacaac aacgccacca
    16381 atactgaccg gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg
    16441 taggtcggtt cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc
    16501 gccagcagtg ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg
    16561 aactcaattc ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga
    16621 cgagtggaca acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc
    16681 gtagccgtta aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag
    16741 acagttgacc agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc
    16801 gctatcaatg cctttccaac tgacgccgac cagagatccg ccccagatcc ccacggcgac
    16861 gaaaataacc gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg gagaaataac
    16921 ccgacgcagc ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat
    16981 caggccgatt tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac
    17041 caccggcccc agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc
    17101 actataagtg gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc
    17161 agaaaccaca ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag
    17221 cggcgcatgt ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc
    17281 ccgcccgaac gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa
    17341 cagcatgagt ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc
    17401 ggaacggaac aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat
    17461 tcgcctgcaa cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg
    17521 cgtgatccgc aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc
    17581 ccagcgcgct gttcagctca gaaatcagct tcaccagtac gcccatggtg atgggatctt
    17641 gcccaacaaa aggttcatca aacatgatga gatccggctc cagcgcaatc gcacgcgcca
    17701 gcgctgcacg ccgcgccatc ccaccagaaa gttcagaagg cattagctta gccgctccac
    17761 gcagccccac ggcctccagc ttcatcatca ctgtactatg caacaatggc gcgggaagtt
    17821 gggtatgttc gcgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg
    17881 ccccggactg aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca
    17941 tcgccggaat attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc
    18001 caatcagacg gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct
    18061 tccctcgcgg cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac
    18121 tgacatcgcg catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt
    18181 gcgttgattt tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata
    18241 ttacccgccg tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa
    18301 aattaagaat ccgttacggc tttctgaaaa tcttcagcgg accggcgagt atacctgaag
    18361 aaaggatgtt agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt
    18421 tacagtgccg accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg
    18481 ccgctgatca tcggcatgac ggtggtcagt attggtacat cgttaccaga agtcatcgtc
    18541 tcgcttgccg cgtctctgca cgaacaacgc gatttagccg ttggtacagc cctcggctca
    18601 aacattatca atatattgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc
    18661 cattctgatg ttctacgccg tgaattaccc ttaatgttgt tggtcagcgt ggtggccggt
    18721 tccgtactct atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct
    18781 gtgctatggc tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac
    18841 agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt
    18901 ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac
    18961 gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt
    19021 gctatcggaa ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa
    19081 aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg
    19141 ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag tcgtgactac
    19201 agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccacaa
    19261 ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg
    19321 tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt
    19381 acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg
    19441 cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt
    19501 ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa
    19561 aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc
    19621 cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta tctctaactc
    19681 aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt
    19741 aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg
    19801 tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc
    19861 cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc
    19921 tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt
    19981 aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg
    20041 tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa
    20101 tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt
    20161 ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac gcgtgcgccc
    20221 tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat
    20281 ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac tgcgtgcagg
    20341 cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc
    20401 tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg tctgctgatc
    20461 ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa cggcgaagag
    20521 ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt
    20581 gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg
    20641 gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag cgatttgctg
    20701 gaaaaactgg cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg
    20761 ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc
    20821 ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc
    20881 gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta
    20941 aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcatta
    21001 atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccatctata
    21061 aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga
    21121 ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac
    21181 ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc
    21241 tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca ctcgtgccgg
    21301 actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg
    21361 ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa
    21421 tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg
    21481 aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc
    21541 agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac
    21601 cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc
    21661 cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa
    21721 ggtaaagaag tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac
    21781 ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc
    21841 gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc
    21901 acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc
    21961 accgttctgg tgccgtcgca gctgcaggat aaaaacaaca aaggccagac cccggcacag
    22021 aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat
    22081 aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt
    22141 ctgctggggc caaacggtgc cggtaagacc accactttct atatggttgt aggcattgtg
    22201 ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat
    22261 gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt ccgtcgcctc
    22321 agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa
    22381 cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc
    22441 atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct
    22501 gcgaatccga aatttattct gctcgacgaa ccgtttgccg gtgttgaccc gatctcggtt
    22561 atcgacatta aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt gctgatcact
    22621 gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg
    22681 catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta
    22741 taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga
    22801 gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga
    22861 cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg
    22921 agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact catgaagaaa
    22981 tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa
    23041 aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta
    23101 caccatccgg caccagcggt gactacattg acgatgagct gccggtctat cagggcgaaa
    23161 cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg ttttccgaca
    23221 ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgacaccggt tatctgactg
    23281 tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg
    23341 aagccgtcct taagcggatc caacggtttg atccggtcgg tgtagcggca aaagatctgc
    23401 gtgactgcct gctaatccaa ctctcccaat tcgataaaac cacgccgtgg ctggaagagg
    23461 ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc cgcactttaa
    23521 tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc
    23581 tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg
    23641 tgctggtgcg taagcataac ggtcactgga cagtagaact caacagtgac agcattccgc
    23701 gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgctcgcaat gatggtgaca
    23761 gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc
    23821 gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg
    23881 agcaaggcga agaatatatg aaaccgatgg tactggccga tatcgcccag gctgtcgaaa
    23941 tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca
    24001 tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc ggcgaagctt
    24061 cctccacggc gattcgtgcg ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac
    24121 cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc
    24181 gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacagc
    24241 tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc
    24301 gagatcaccg aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat
    24361 tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca
    24421 gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg
    24481 tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat
    24541 aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg
    24601 cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg
    24661 ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac
    24721 agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc
    24781 agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga agctatcctg
    24841 acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgcaattcc gcatggcaaa
    24901 ctggaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac gcctatagct
    24961 ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt gccggcagac
    25021 caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc ggacaaaacc
    25081 atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat
    25141 accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag
    25201 gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc
    25261 gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg
    25321 ttacccgatc tggctcgaac cctggccgat cgcgagattt ctgccgccgt cagcattgat
    25381 gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac
    25441 gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat tcgtcgttac
    25501 agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc
    25561 gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc
    25621 tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct gggtaaacgt
    25681 gaacgcgaac tgacaatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat
    25741 gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt
    25801 ccaatgacag gtcttgataa gcccgtcgcc gcgttcctcg accgccacac agaagtacac
    25861 aattttatct accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac
    25921 aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat
    25981 attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat
    26041 cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc
    26101 tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg
    26161 aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt attgcgctgc
    26221 tgatgttaga ttctgccaaa ggacggcaga ttgaagttga agcgaccggc ccacaggaag
    26281 aggaagcact ggcagccgtt atcgccctct ttaactctgg ttttgatgaa gattaatctt
    26341 catcactttg acatacaact cccttcaaac tccccccgac aataagaaaa tcacgtactg
    26401 aaatcgttct caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc
    26461 ttaagaaaac cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag
    26521 atcgtcagaa aatatttcca ggagatggca tgattcgctt atctgaacaa aatcccctgg
    26581 gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga
    26641 ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg
    26701 cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg
    26761 agacggattg cggaaccggt tatgtctacg atgtgattgc agattttgac ggcaaaccct
    26821 ccattacgct gaccgaattt gctgagcaat gccgttacga agaagacatt gcacaactgc
    26881 gccagctact gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc
    26941 tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct
    27001 gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc
    27061 tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc
    27121 tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt
    27181 aatccagctg gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa
    27241 tccacgcctg acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat
    27301 taggtaatac agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt
    27361 ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat
    27421 tcaggtaaac cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc
    27481 cagcttccag cccttttcgt acccagctac gcccatccca taaaaagaga tttttggccg
    27541 tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca
    27601 gggctttctc aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg
    27661 cggcaatcac cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat
    27721 gtgccacgta acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg
    27781 ctgagaaggg aacaggcgca acactaaaca acgcgatgcc cccgccccag aaaacggcga
    27841 ggacaaccat taaccgcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt
    27901 tactcattca gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc
    27961 tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac
    28021 cacgatatca tcgacaggac agggcacatg ctccgcgccc atctcttcca gcacttctgc
    28081 ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat
    28141 cgctggggca atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag
    28201 cgcctttaat tcacggtcaa cggtgcattc gctaccaaga gtggcaaaat tgcttaaatt
    28261 cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg
    28321 ggccagagga cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cgttgcgcgt
    28381 ttccgtcatc gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc
    28441 aaagcagacc gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg
    28501 aatttcagaa ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt
    28561 gatcatcctt tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc
    28621 acacatttaa ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg
    28681 cctgaaaaac agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat
    28741 ttccctggtg ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg
    28801 cttttgccac ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt
    28861 caatccattc accgacgtta tcttcccagg caggaaggtc cggagactga atttgctgac
    28921 ccagctgttg cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt
    28981 cctctacaat gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc
    29041 ccggcatcat cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt
    29101 cgagatactg ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg
    29161 tcgtcaccgt actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta
    29221 gcgccggaac cgaaagcggc ttactcagca catcatccat tccagcattg aggtactctt
    29281 gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat
    29341 aacgcttcgt cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca
    29401 gcaacaccag gtcgtattcg cccggtttaa acatctccag cgccgccttg ccggtcatgg
    29461 cgacatcaac gctgttacct aatttttcca gcacagaacg cgcaacaatc acgttcagtt
    29521 caatgtcttc caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg
    29581 catcatcgac ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt
    29641 tgccctgttc gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa
    29701 cggccagacc aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt
    29761 aatacatggc gaaaatttta tccagctcat cctgcggaat gccgatacca gagtcttcca
    29821 cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct
    29881 gggtgaattt gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt
    29941 cggtaatgac ctgatgcggt aatggcagcg tcggctccag gttaaagcgc aatccttttt
    30001 gttgcgcctg caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg
    30061 gttgattatc aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa
    30121 agatattccc cagcgtgacg gccgaaacat ggatggtctt gagatatttt tcctgctcgg
    30181 cggtgagttc ggtatccagc agaatacggc tcaggccgac gataccgttc agcggtgtac
    30241 gcaattcgtg actgatggtg gagataaacg tcgttttgtc gcggctggcc cgttcaagcg
    30301 catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt
    30361 tacccacgcg gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg
    30421 ggtaatccag ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg
    30481 tttcaatgac ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca
    30541 gttgtttttc gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt
    30601 tatcttcgtt acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg
    30661 aggattgctg ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt
    30721 ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg aacggcgatt tcctgattta
    30781 gctgggcaat attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct
    30841 ccagtttttg caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca
    30901 ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata gaacgaataa
    30961 catcaatgct ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg
    31021 caagaacgac gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca
    31081 tcaggtcaac ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg
    31141 acaacctgcc ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc
    31201 ggaattcccc tcaacgacct actccgttgg aggtatccac ggacgttcca gcgccgatcc
    31261 ctgtacgcca tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc
    31321 gcaccacaga ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat
    31381 cacttccggt ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc
    31441 aataccgttc aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg
    31501 cagcggatgc agctttatgc cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa
    31561 acattcagcc tgcccttcgc caggcaggcc gacaattaag tgggaacaca ccttcagccc
    31621 gcgctgacgt gccagctggg ttgtacgctg ataacaggca aaatcatggc cgcggttgat
    31681 gcgatgcagt gttttgtcgt gggcggtttg tagccccagc tccagccaca cttcgtaacc
    31741 ctggtcctta tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgggt
    31801 accaacacac aaaccgacaa tattggcctg gctcaccgcc tgctgataca tagaacgcag
    31861 cacctgaact tccgcaaagg tgctggtata cgcctgaaag taggccagat agcgtttagc
    31921 gcggttaact aaattcgcct ggtgcgccag ttgctcggca atggaacgat gctgctgcgc
    31981 ttcatcggca aacgaggcaa cattacagaa tgtgcagcca ccacgcccga tggtaccgtc
    32041 acggttaggg cagctaaaac cgccgtgtag cgtcagctta tgcacctttt gcccataacg
    32101 acgggtgaga tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac
    32161 cgcgccttga aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat
    32221 caatcgtccc ggcctgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg
    32281 cataatcaca taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat
    32341 tttaggcaaa aacagtagca tgaaacgtca ttaccaatta aggcagtata aaatgctggt
    32401 tttctcgtca gttcaaggca ggataagggt taacacgcct ttatgacagt caggaattga
    32461 ctgtttctct aacgacttcc cttttagcct taaagataaa atccatttta atttcagtaa
    32521 tttaataaag aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct
    32581 gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct
    32641 catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga
    32701 caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt
    32761 ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg gaggttcacg
    32821 atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca
    32881 tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc
    32941 agcaccgtgg cgcgattctc gccgatggta aaaccggcga cggttgcggc ttgctgttac
    33001 aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagccaaaa
    33061 actacgctgt cgggatgatc ttcctgaata aagactctga actcgccgct gccgcacgcc
    33121 gcaccgttga agaagagctg caacgcgaaa cgttgtcgat tgtgggctgg cgtgatgtcc
    33181 ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa
    33241 tttttgtgaa cgccccggca ggctggcgtc cgcgtgatat ggaacgccgt ctatttatcg
    33301 cccgccgccg catcgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt
    33361 cgaatctggt caatatttat aaaggtctgt gtatgccggc ggatctgccg cgcttttacc
    33421 tggatcttgc ggacctgcgc ctggaatcgg ccatctgcct gttccaccag cgcttctcca
    33481 ctaacaccgt accgcgctgg ccgctggcgc agccgttccg ctatctggcg cataacggtg
    33541 aaatcaacac catcaccggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga
    33601 cgccgcttat ccctgatctg cacgacgcag cgccattcgt caacgaaacc ggttctgact
    33661 ccagctcgat ggataacatg ctggaactgc tgctggcagg cgggatggat atcatccgcg
    33721 ctatgcgtct gttagtgccg cccgcctggc agaacaaccc ggatatggac ccggagctgc
    33781 gtgccttctt cgactttaac tccatgcata tggagccgtg ggatggcccg gcgggcatcg
    33841 tgatgtccga cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc
    33901 gctacgtcat caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt
    33961 atcagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggtgatcg
    34021 acacccgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc
    34081 atccatataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc
    34141 ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac
    34201 agtttaacta cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc
    34261 aggaagcggt cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc
    34321 gcattattta cgactacttc cgccagcagt ttgcccaggt gactaacccg ccaatcgacc
    34381 cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct
    34441 tttgcgaagc agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact
    34501 ccgatttcaa acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata
    34561 tcacctttga cgtcactaaa accacgctcg aagcgacagt caaagagctg tgcgacaaag
    34621 ccgaaaaaat ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta
    34681 aagatcgcct gccggttcca gccccgatgg cggttggcgc gatccagacc cgtctggtcg
    34741 atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc
    34801 accacttcgc cgtgttgctg ggcttcggcg cgacggctat ttatccatac cttgcctatg
    34861 aaacgctggg ccgcctggta gacacccatg cgattgccaa agattatcgt accgtgatgc
    34921 tcaactaccg taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct
    34981 ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtctg cacgatgatg
    35041 tagtgggcct gtgcttccag ggggcggtca gccgcattgg tggggcgagc tttgaagact
    35101 tccagcagga tctgctgaac ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc
    35161 agggcggcct gctgaaatac gtccacggcg gcgaatacca cgcttacaac ccggacgtgg
    35221 tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga
    35281 agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg
    35341 aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa cgctttgata
    35401 ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga
    35461 tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg cgaagacccg gcgcgctatg
    35521 gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg tcgcttcggc gttactccgg
    35581 cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg
    35641 aaggcggtca gttgccgggt gataaagtca ctccttacat cgccaaactg cgctattcgg
    35701 tgcccggagt gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaggact
    35761 tagcgcagct cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc
    35821 tggtttccga accgggcgta ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt
    35881 taatcaccat cgcaggctat gacggcggca ccggcgcaag tccgctttca tcggtgaaat
    35941 acgcaggctg tccgtgggag ctggggcttg ttgaaaccca gcaggcgctg gttgctaacg
    36001 gcttgcgtca caagatccgt ttgcaggtcg atggcggcct gaaaacgggt gtcgatatca
    36061 tcaaggcggc gattctcggc gcagaaagct tcggcttcgg cactggccct atggtggcgc
    36121 tcggctgtaa atatctacgt atttgccatc tgaacaactg cgcaacgggt gtagcaactc
    36181 aggatgacaa actgcgtaag aaccactatc acggcctgcc gttcaaggtt accaattact
    36241 ttgaatttat tgcccgtgaa acccgcgagc tgatggcaca gctgggcgta acgcgtctgg
    36301 tggatctgat tggtcgcacc gacctgctga aagaactgga cggtttcacc gccaaacagc
    36361 aaaaactggc gctgtcgaag ctgctggaga ccgccgaacc gcatccaggt aaggcactct
    36421 actgcaccga aaacaacccg ccgtttgata acggcctgct gaacgcgcag ttgctgcaac
    36481 aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat atccgcaata
    36541 ccgatcgctc cgtgggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg
    36601 ggctggcagc cgatcctatc aaagcgtact tcaacggcac cgcaggccag agtttcggtg
    36661 tatggaacgc gggcggcgtg gaactgtacc tgaccggtga tgccaacgac tatgtcggta
    36721 aaggcatggc gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc
    36781 atgaagcaag cattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg
    36841 ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc accgtggtag
    36901 aaggcattgg cgacaacggc tgtgaatata tgacgggcgg tattgtctgt attctgggta
    36961 aaaccggcgt taacttcggc gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa
    37021 gcggcgattt ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgatc
    37081 tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag cataccggtt
    37141 cccagcgcgg tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg
    37201 ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca ccgtagtcgt agcgcagctg
    37261 agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta
    37321 tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg
    37381 ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt
    37441 cgtgcggcaa cccatactgc gagtggaaat gcccggtaca caactacatc ccgaactggc
    37501 tgaagctcgc caacgagggg cgtatttttg aagcggcgga actgtcgcac cagaccaaca
    37561 ccctgccgga agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca
    37621 ctctgaacga tgagtttggc gcggtgacca tcggcaacat tgagcgttat atcaatgata
    37681 aagcgttcga aatgggctgg cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag
    37741 tggcgattat cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg
    37801 gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg cggactgctg accttcggta
    37861 ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca
    37921 tgggtattga attcaaactc aataccgaag tgggccgcga cgtgcagctg gacgatctgc
    37981 tgagtgatta cgatgccgtc ttccttggcg tcgggactta tcagtcaatg cacggcgggc
    38041 tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca
    38101 aacagttaat gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac
    38161 gcgtggtggt ccttggcggt ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc
    38221 agggagcgaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc
    38281 gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccac
    38341 tggggattga agtgaacggt aacggcaaag tcagcggcgt aaaaatggta cgtaccgaaa
    38401 tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata
    38461 tcgttccggc agatgcggtg atcatggcgt ttggtttccg tccacacaac atggaatggc
    38521 tggcaaaaca cagcgtcgag ctggattcac aaggccgcat catcgccccg gaaggcagcg
    38581 acaacgcctt ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt
    38641 ccgatctggt ggtaaccgct attgccgaag gtcgtaaggc ggcagacggt attatgaact
    38701 ggctggaagt ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg gcgctcaaat
    38761 tctcctttct ttctggaaag cgcctcgcat aatctgaaga aaaccaatgt aatacgctca
    38821 tctggattgt taccggtaga tgcccggcgt atagctgatt ccattattca acgcagcgcc
    38881 atataaaggg acagataatg gaatcgctct cagagagaac ctcaacaggc taccagcaaa
    38941 tccatgacgg tattattcac ctggtcgata gcgcccggac ggaaacggta cgtagcgtta
    39001 acgcgctgat gaccgcgaca tactgggaaa ttggccgacg aattgtcgaa tttgaacaag
    39061 gtggcgaggc cagggctgcg tatggtgcgc agctaatcaa gcgactatca aaggatttaa
    39121 gtctaaggta taagcgtggt ttctctgcaa aaaacttacg ccaaatgagg cttttttacc
    39181 tcttttttca acatgttgat ttggcagacg ctgtctgcca aattgagagg ctgtttgata
    39241 tttccacagc ttttgtattg catctcctat tttcggtttg tatattcatg caaataaacg
    39301 aaggaaagtt gccatgattt acattgaaca gcttgaatta atccataaaa gtggtgatgt
    39361 gttgtatcca gtaaaaataa ccagaaaatc atcaggaaaa actgccttcc accttgttcc
    39421 attcggctta aataagactc acgaccttct agaggtggaa gatgcttcag aagcaattcg
    39481 gctggtgatt gatgaacgtc attccattcg ctgcagtaca cttacagcaa ctattacgaa
    39541 taaaaagggc aagcgaatta agcggacagg tatatacagt atcaagggtg ttaacataaa
    39601 agaatacaac gtcaggtaat ggcacagagc ggtccgtcag ttcaggtttg gctctgtgcc
    39661 aaagatgggt caggtcacac ctgatctaat aaatttaagc cattaaatca gccttaacct
    39721 tcaccaccac ctttttaatc tcacccggct cgccaacaac gcatcccggc ttatgcggct
    39781 ctcctggcat aaacacggcg aacattcccg gtttcaggat gatggtttgc tcgttttcaa
    39841 tggcgctaca aagctggtaa tcatcttcat ggtggaactc ttcacattgg cgcgcagtgc
    39901 ctgccatgcc aaacagaatc cgttcctcac cgtttaataa cagctggata tcaatgtatt
    39961 gctcgtgcaa ttccgctttt ttctcgacgg gcgattgagt gttaaacgtc atgacattca
    40021 taaaaatatt gtcgccctgt aattcgtaac gacccggcgc tttttcttgc ggtctggcag
    40081 ctaaggccag cgttaacgcg tcctgtaacg caggatgtaa cccagcagac ggtaatgact
    40141 gtacttcacc catcatcata atttttctcc ctgggccaac agcgcagccc caagtaaacc
    40201 tgcatcatgg cggtaatgcg ccgccagtaa atcaacatga aatgccgctg gctcctgcgc
    40261 cagatacatt tccaccaacg ccagataccc ttctgccaga ccaacgctgc caccaaccac
    40321 cacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcaa gcgtacgtgc
    40381 ggagagatga atcagttgct gcgcctgctc atcgccctgc ccggcgcgtg tgaaaatagt
    40441 tctagcatcc gcgccagcca gctcgccctg cgctgccgcc gcaatgccgc gaccagaagc
    40501 aattgcttcc acgcaacctg tgcgtccaca gccgcagact gggccgtgtg gatcggcaag
    40561 cgtatgcccg atgtgccccg ccagaccgcc agggccggta agcagtttgc cgccgctcac
    40621 tacaccgccg ccgacgccag tggaaacggt gataaagacc atatcggtta tatcgccttc
    40681 cagcgcctga tactctgccc atgctgcggc ctgcgcgtcg ttaatggcaa tggtcggcaa
    40741 attggtaagt tgttccagcg ttttgactaa cggaaagtgt agcaatccac cgagattatg
    40801 cggattcagc gccagcaaac tgccgtcgcg aataattccg gttgaagcga tggcaacccg
    40861 ctgcgcatga gcttgcaacg gagagactaa tgcggataag gcatcacgca aggcttctgg
    40921 tgtctggctg gctggcgtag gaagttcacg acgatcgcgg atctgcccgt cagcgccaat
    40981 cagcgcggcg gcaagtttag taccgccgat atcaatcgcc agtgtggtca tagcaccgcc
    41041 tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat ggcagaaccg
    41101 accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg cgtgttgtaa
    41161 cgcccttcgg caatcacccg acatccggcg tcgctcaacg ttttcaccag cgccagatcc
    41221 ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat aatttcggct
    41281 cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat cgccagtaaa
    41341 ccgtgatggt gaatacgtgc cagcagcgtt tcaacaggca ccggacgcgg gcggtcggtg
    41401 ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcgacatc ttcaatatag
    41461 gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caattccaat aatcggcacg
    41521 ctcaccaccg cacgcgtggc ttgcagattt gccacacctt caatgcgaat ggcaaccgcg
    41581 cctgcctgtt ctgccgctaa tgccatggcg gcgacgattt cgggtttatc gagcgggctg
    41641 tccggaaccg gctggcagga gacaatcagg ccaccgttag cagcgatttt ttgatccagt
    41701 tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacatag accgggcaac
    41761 aggattaact tttggttttg actaaatcgt ttttggcgct gccaaatggc acggcaccgc
    41821 taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc agccaacgct
    41881 gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc agactgaacg
    41941 atagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg ccgatgattg
    42001 gggccagtgc gccgcccaat gcgccaacgt tgtaggtaaa gcccaggccc gctgcacgct
    42061 ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc ccttgtccaa
    42121 gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg ccaatcgcaa
    42181 ataccggaat aatcaacagc tgcgaggcca acaggctaca aacgtaggct ttgcgggttc
    42241 ccagccagtc accgaggaag ccgccgacac agcaccccac cgcagcccca aagccgctaa
    42301 agaacagcac attggcgaca gtatgcgggt tataagccag atcggttttc agatacgttg
    42361 gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc accatcagca
    42421 ttacgcccgt tggccagcgt ttgcctgtac tctgcaccat aaagctgata aagattgcgg
    42481 cgcataacag cccaagaaca gcgacgatcg cggcattttg caggttaccg gcgaagcaga
    42541 accacagcgc agtagccgcc gccagtgtca ttacgatatt ggcaatgcga tgttcaccac
    42601 ggtagagaat atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc tctttccagt
    42661 cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgattggc aaaatgccga
    42721 taaagaacag cgcacgccag ccccagaccg gaaccaccag gctatagacc tgagcggcaa
    42781 cgacggcccc cacagagaag cctgaaatca gaaaaccact ggctttgtta cgcagatgtt
    42841 ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc atccccatgc
    42901 cgatgaccag acgcgcgata aacatggtga tgtagcctgg cgcaaagccg caggccagcg
    42961 tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg tagcggtcac
    43021 ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg gagatcagac
    43081 ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt accagggcga
    43141 ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa aatgcgcgcc
    43201 attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta ctcattgtga
    43261 gtctcccgcg gtgggcgatg cccacacgct ttggtataaa aattgtaggg tacagatgcg
    43321 tttatttccc ctcacccggt aggggcgagc gaggggaaac aactcacccg cgctcttgca
    43381 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
    43441 gtttgcggca cagcggcaca gaaacgacat ccatataatg gaggacagtt ttcaggccgc
    43501 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
    43561 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatag cgccagccca
    43621 tgatgttgta ggtactaccg ataccaccat cagcgcccgc cagcagacca gaggcgaaga
    43681 tttcgtcata accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
    43741 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
    43801 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
    43861 gcaaaccatc cgccgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
    43921 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
    43981 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
    44041 gacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
    44101 caaaggcctc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
    44161 actgaaccag gcgacgcaga ctcgctttat ccagtgcttg ttgttggtca taaggagtca
    44221 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
    44281 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
    44341 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
    44401 tgaccgaagg cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
    44461 gcttcgtcag gatcgtggcg gcggatcgcg tcaacgatcg caatatgctg ttgatagcta
    44521 acgttgttat gctcgtgcag agcctgatca gccacggttg gacgtgcggc gattagccag
    44581 tcgagcaggg caacgtggat cgccatgaag attgggttac ctgggatctc cgccagcacg
    44641 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
    44701 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
    44761 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
    44821 tgagaaagga aatctttcgc catgccggaa agctcaccga tgatagtgtc cgcagaagga
    44881 cgcgagacgc gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
    44941 gccagcgctt cgcgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
    45001 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
    45061 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
    45121 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
    45181 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
    45241 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
    45301 aaattaatgt aacgcagata aaaacatccc gtttgaatta ttcataagac tattcacgag
    45361 cattatgaat attatgaatg tgttcttaca aaataatcat aagcgcatat tttttaatga
    45421 aaaaccacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
    45481 atataacatc attgttttca atctgccgtt tatgggattg accgttttct tttgacacgg
    45541 agttcaacaa tgttcggcat aattatatct gtcatcgtat taattacgat gggctatttg
    45601 atcctgaaaa actacaaacc tcaggtggtg ctggctgccg caggtatctt cctgatgatg
    45661 tgcggtgtct ggttagggtt cggtggtgta ctcgatcccg ccaaaagcag cggctacttg
    45721 atcgtcgata tttataatga aatcctgcgc atgctgtcca accgcattgc cggattgggg
    45781 ctgtcgatta tggcggtggg cggttatgcc cgctacatgg agcgcacagg agccagtcgc
    45841 gcgatggtga gcttgctaag ccgcccgtta aaactcattc gctcgccgta tattattctg
    45901 tcggcaactt acgtcatcgg ccaaatcatg gcgcagttta ttaccagcgc ctccggcctg
    45961 ggtatgttgc tgatggtcac cttatttccg acgctggtga gtctgggagt aagtcgcctc
    46021 tctgcggtgg cggttatcgc aaccacgatg tccattgagt gggggattct ggaaacgaac
    46081 tccatttttg cagcccaggt cgcgggaatg aaaattgcca cttacttctt ccactaccag
    46141 cttccggtcg cctcttgcgt cattatctcg gtggcgatct cccacttttt cgtgcaacgc
    46201 gcttttgaca aaaaagataa aaatatcaat cacgaacagg cagagctaaa agctctcgat
    46261 aatgtcccgc cgctctatta cgccatttta cctgtgatgc cgttaatctt gatgctcggc
    46321 tcgctgttcc tcgcccacat cgggctgatg cagtcagaac tgcatctggt ggtggtgatg
    46381 ttactgagtt tgacggtgac gatgtttgtt gagttcttcc gcaagcataa cttgcgcgaa
    46441 acaatggacg atgtgcaggc gttttttgac ggcatgggta cgcagtttgc caacgtggta
    46501 acgctggtgg tcgcgggtga aatatttgcg aaaggcttaa cgacgattgg cactgttgat
    46561 gcggttatca ggggtgcgga gcattctggt ctggacggta ttggcgtgat gattattatg
    46621 gcgctagtca ttgccatttg tgccattgtg atgggctctg gcaatgcgcc gtttatgtca
    46681 tttgccagtc ttattccgaa tatcgcagcc ggactacatg taccagcggt tgtaatgatt
    46741 atgccgatgc attttgccac gacgctagcg cgcgcggttt cgccgattac tgcggtggtg
    46801 gtcgttacgt caggaattgc aggcgtttcg ccttttgcgg tggtgaagcg aacagcgatc
    46861 cccatggcag tcggtttcgt ggtgaatatg attgccacaa tcacgctatt ttattaagcc
    46921 attaaaagac aaaacaggcc accagggcct gtttttgtat tacttcacaa cgcgtaatgc
    46981 cggtcgacca ccgcgtggtg gctgcggagg ttcatcgtca ggatgagtgt catcatcgtg
    47041 atctggcttg tcgccatcaa taaccgacat aacggtttcg ttgtctgccg atgcctcttc
    47101 atcattcatg atgctggtat cttcatcgta ggcagcttca ggctcaaaca tcgtgcctgc
    47161 gccattttca cgggcgtaga tagccagcac ggcagccagc ggcacagaaa cctgacgcgg
    47221 aatgccgcca aagcgcgcgt taaagcgtac ctcatcattc gccagttcca gattgccgac
    47281 agcacgcggc gcaatgttga gtacgatttg cccgtcacgc gcatattcca taggaacctg
    47341 cacgccaggg agcgtcacat ccaccaccaa gtgcggcgtg agctggttat ccagcaacca
    47401 ctcatagaat gcacgcagca gatagggacg acgtggtgtt agctgtgaca aatccataca
    47461 gattaactcc ggcccagacg catttcacgt tctgcttcag ttaaagaagc aaggaaagag
    47521 tcacgctcaa agacgcgggt catatagcct ttcagctctt tcgcacccgg gccgctgaac
    47581 tcgatgccca gttgcggcag acgccacagc agcggagcaa gatagcaatc gaccaggctg
    47641 aactcatcgc tcaggaagta cggcttctga ccgaagaccg gcgcaatcgc cagcagttct
    47701 tcgcgcagtt gcttacgtgc ggcatctgct tcagaagctg aaccgttgat gatggtgttc
    47761 atcagcgtgt accagtcttt ttcgatgcga tgcatgtaca gacggctttc accgcgagct
    47821 accgggtaaa caggcatcag tggcggatgc gggaaacgct catccagata ttccataatg
    47881 atgcgagatt cccacagggt cagctcacga tccaccaggg tcggaacgct ctgattcggg
    47941 ttgaggtcaa tcagatcctg aggcggattg tccttttcca cgtgttcgat ctcgaaactt
    48001 acacctttct cagccagcac aatgcggacc tgatggctat agatgtcagt aggaccggaa
    48061 aacagcgtca ttaccgaacg tttgttggca gcgacagcca tgaaaacctc caggtatagt
    48121 cagaattttt actgctacca gccaccaggt ggccagtcag aagttgtgtt acccagtaag
    48181 gaacgactct ctttgttcga aaatcaaaca aaaaatgagc aatacccgac atttgggcag
    48241 aaaattggat gatagtttac cagattttgc gacctttgtg gtgagtcgat gccggaaatg
    48301 gggaaaaaga gatgcgcttt agtctgaaat agttgactta gtcccttatt ggcgatgtgg
    48361 tttttgtttt gcctgtctgt cagacggtag caaaaagcaa ctttccaatt tttacgctga
    48421 ttcagaattt aaccataaaa aaacccgccg aagcgggttt tttcgcaaat tgttttctgc
    48481 cggagcagaa gccaattaac gtttggagaa ctgcggacga cgacgtgctt tacgcagacc
    48541 gactttctta cgttcaacct gacgagcgtc acgagtaacg aagccagctt tacgcagttc
    48601 agaacgcagg gactcgtcgt attccatcag agcgcgggtg ataccgtgac ggatcgcacc
    48661 agcctgacca gagataccac cacctttaac ggtgatgtac aggtccagtt tctcaaccat
    48721 gtcgaccagt tccagcggct gacgaactac catgcgagca gtttcacgac cgaagtactg
    48781 ttccagagaa cgttggttga ttacgatttt accgttgccc ggtttgatga aaacgcgagc
    48841 tgcggaactt ttgcggcgac cagtgccgta gtattgattt tcagccattg cctataatcc
    48901 cgattagatg tcaagaactt gcggttgctg tgccgcgtgg ttgtgctcgt tacccgcgta
    48961 aactttcagt ttacggaaca tagcacgacc cagcgggcct tttggcaaca tgcctttaac
    49021 cgcgatttca atcacacgct caggacggcg agcaatcatc tcttcaaagg tcgcttgttt
    49081 gataccaccg atgtggccgg tgtggtgata gtacactttg tcagtacgct tgttgccggt
    49141 tacagcaact ttgtcagcgt tcagaacgat gatgtaatca ccggtatcta cgtgcggagt
    49201 gtattccgct ttgtgcttac cgcgcaggcg acgagccagt tcagtagcca gacggcccag
    49261 agttttaccg gtcgcgtcaa caacatacca gtcgcgtttt acggtttctg gtttagctgt
    49321 aaaagttttc attaaaagct tacccaataa atagttacac gttggtgaac acccaaacgt
    49381 cttcaattgt tgaggttcac acgacaaagt ccggcaaacc taccccttcg aatagcctat
    49441 gccagcacac aaaaagtttt gggaaaaaaa ctttcttgta acgtggggtc gcaggattat
    49501 agagaagtcg gggtcaaaga tcgacccctt tttgtgattt gtgacaggtt ttaacccgcc
    49561 aaatgctcgc gcttcagata ctcttcgctt tgcatctctt gcagacgtga caggcaacgc
    49621 tggaactcaa acttcagccg atcgccctga taaatttcat acagcggcac ttctgcactc
    49681 accactaatt tgacatggcg ctcgtaaaac tcatccacca gcgcaataaa gcgccgcgct
    49741 tcgctctcca tcaaccgcgt cataactggt acatcaaaca acatgaccgt atgaaagaga
    49801 cgtgagagcg caatatagtc atgctgactg cgggcgtcga cgcacagcgt agtaaaagag
    49861 accgccagcg tctggttctc gacgcccatt gtcgccaatg gccgatggtt gatttctaac
    49921 gtcggtgaat tttctcgttt cgcccccgcc agcgccaacc atagtttatc catctgcgcc
    49981 cgggtttcat cgttgagtgg cgaaagccac agatgcgcct gagtgagtgt acgcagtcga
    50041 taatcaacac cagcgtccac gttcattaca tcacaatgct gtttaatggc atcgattgca
    50101 ggcagaaaac gcgcacgttg caggccattt cgataaagtt catccggcgg aatatttgac
    50161 gtcgctacca gggtaatgcc gcgggcgaac agggctttca tcagaccgcc aagtagcatg
    50221 gcgtcggtaa tatcagaaac aaaaaattcg tcaaaacaga gcacgtcagt ttcggcttta
    50281 aagcgatcgg caataatttc cagcggatcg gtctgcccct gtaaggcagt tagctcttcg
    50341 tgcatccgct gcataaaacg gtgaaagtgc aggcgctgtt tccgctctcc cggcaggctt
    50401 tgatagaaaa ggtccatcag ccaggttttc ccgcgtccta caccgcccca catatataag
    50461 ccacgcactg gcgtatgctt tgtgtcttcg cgtttacccc acagcttacc gacccgcgcc
    50521 attagcccac tcgtcctggg ggctggtggc ctgctattga tgagttcttg ataaataatt
    50581 tccaggcggc tgacggcctc tttttgaacg tcgtcgggtt gatggctgcc ttcattgagc
    50641 gccttcaggt attgcgatgt tggggtaacg ctttgcatga tcttattgtt attccttgaa
    50701 taatcggtgc gccgttgttc acggttgacg aaaaaaaggc cgttctacac tacgcgatat
    50761 gcagtcggga ttccacttct gtggaattaa cggttatagt ggcataatca gccgcaggca
    50821 tggagcctga agccaacacc ctacggaaac aaaagacaac gggagatgtt catgacctgg
    50881 gaatatgcgc taattgggtt agtcgtcggc atcattattg gtgctgtggc catgcgtttt
    50941 ggtaatcgta aactacgcca gcaacaggcg ttgcagtacg aactggaaaa gaataaagct
    51001 gaactggacg agtatcgcga agagctggtt agccactttg cccgcagcgc ggaattactg
    51061 gataccatgg cgcacgacta tcgccagctg tatcagcaca tggcgaaaag ctccagcagc
    51121 ctgctgccgg aactgtctgc tgaagcaaac ccgttccgta atcgtctggc agagtctgaa
    51181 gccagcaacg atcaggcacc ggtgcagatg cctcgcgact attctgaagg cgcatccggc
    51241 ctgctgcgta ctggcgcgaa gcgcgactaa tttatttttc gggcgcagcc attgcgccct
    51301 cctcttctct ccctccccga ctatcattta atctggtgtc tcattgttag ccgtctgaaa
    51361 attcaataac atcaaactgt tttgaatctc ttttcttatc attcaggtac gagagcagga
    51421 ataatgaaaa aacaaaccca gctgttgagt gcattagcgt taagtgtcgg gttaactctc
    51481 tcggcgtcat ttcaggccgt cgcgtcgatt ccaggccagg ttgccgatca ggcccctctc
    51541 cccagtctgg ctccaatgct ggaaaaagtg cttccggcag tggtgagcgt acgggtggaa
    51601 ggaacggcca gtcagggaca gaaaatcccg gaagaattca aaaagttttt tggtgatgat
    51661 ttaccggatc aacctgcaca acccttcgaa ggtttaggtt ccggtgtcat catcaacgcc
    51721 agtaaaggct atgtgctgac caacaaccat gtgattaatc aggcacagaa aatcagtatt
    51781 cagctcaatg atgggcgcga gtttgatgca aaactgattg gtagcgatga ccagagcgat
    51841 atcgccctgt tacaaattca aaacccgagc aaattaacgc aaatcgctat tgccgactcc
    51901 gataaattgc gcgtcggtga ttttgccgtg gcggtcggta acccatttgg ccttgggcaa
    51961 accgccacct ctggcattgt ttccgcatta ggacgcagcg ggttgaatct tgaaggtctg
    52021 gaaaacttta tccagacaga tgcttccatt aaccgcggta actccggcgg tgcactatta
    52081 aaccttaacg gtgagttaat tggcatcaac actgcaatcc ttgcgcctgg cggcgggagc
    52141 gtcgggattg gatttgccat ccccagtaat atggcgcgaa cactggcgca gcagcttatc
    52201 gactttggtg aaatcaaacg cggtttgtta ggcatcaaag gcaccgagat gagtgccgat
    52261 atcgccaaag ccttcaacct tgacgtgcag cgtggcgcgt ttgtcagcga agtgttgcca
    52321 ggttctggct cggcaaaagc gggcgtcaaa gcgggcgata ttattaccag cctcaacggc
    52381 aaaccgctga atagctttgc tgagttgcgc tctcgtatcg cgactacaga gccgggcacg
    52441 aaagtgaagc ttggcctgct gcgtaacggc aaaccactgg aagtagaagt gacgcttgat
    52501 accagcacct cttcgtcggc cagcgctgaa atgatcacgc cagcgctgga aggtgcaacg
    52561 ttgagcgatg gtcagctaaa agatggcggc aaaggtatta agatcgatga agttgtcaaa
    52621 ggaagcccag ctgctcaggc tggcttgcaa aaagacgatg tgatcattgg cgtcaaccgc
    52681 gatcgggtga actcgattgc tgaaatgcgt aaagtgctgg cggcaaaacc ggccatcatc
    52741 gccctgcaaa ttgtacgcgg caatgaaagc atctatctgc tgatgcgtta atgtcgtaaa
    52801 ccgggcatca ggcttacgtg tgatgtccgg ttaactcgtg gtatgctgct gccgttccct
    52861 tttttaatga cgcctccatc atgtttgtga agctcttacg ttccgttgcg attggattaa
    52921 ttgtcggcgc tattctgctg gttgccatgc cttcgctgcg cagccttaac ccgctttcca
    52981 ctccgcaatt tgacagtacc gatgagacgc ctgccagtta taatctggcg gttcgccgcg
    53041 ccgcgccagc ggtggttaac gtttacaacc gtggtttgaa caccaactct cacaaccagc
    53101 ttgagatccg caccttggga tccggtgtaa tcatggatca acgcggttat atcatcacca
    53161 ataaacacgt catcaacgac gccgatcaga tcatcgtcgc cttacaggat ggtcgtgtat
    53221 ttgaagcatt gctggtggga tctgactctc taaccgatct ggcggtactt aaaattaatg
    53281 ccactggcgg tttacctacc attccaatta atgcacgtcg cgtaccgcac attggcgacg
    53341 tagtactggc gatcggtaac ccgtacaacc tcgggcagac cattacccag gggattatta
    53401 gtgccacggg gcgaatcggt ctgaacccga ccgggcggca aaacttccta caaaccgatg
    53461 cttccattaa ccacggtaac tctggcggcg cgctggtgaa ctcgctggga gaactgatgg
    53521 gcattaacac gctgtcgttt gataagagta acgatggcga aacgccggaa ggtatcggct
    53581 ttgcgattcc tttccagtta gcaaccaaaa ttatggataa gctgatccgc gatggtcgcg
    53641 tgatccgcgg ctacattggt atcggcggac gcgagatcgc accactgcac gcgcagagcg
    53701 gtggtataga tcaactgcaa gggatcgtgg ttaatgaagt gtcacctgac ggcccagcgg
    53761 cgaatgcggg tattcaggtc aacgatctga ttatttcggt ggataacaaa ccggccatct
    53821 ctgctctgga gacgatggat caggtggcag aaattcgccc tggttcggtg atcccggttg
    53881 tagtgatgcg tgatgataag cagttaacgc tgcaggtcac cattcaggaa tatccggcaa
    53941 ccaattaagt cgagtgctca aaacaaaaaa ccggagtctg tgctccggtt ttttattatt
    54001 cgctaattaa ttacttatta acgaactctt cgcccagggc gatatctttc ttcagcgtat
    54061 ccagcatacc ttccagtgcg ctctgttcaa atgcgctcag ggtaccgata gatttacgct
    54121 cttccacgcc gtttttaccc agcagcagcg gttgagagaa gaaacgggcg tactgaccgt
    54181 cgccttcaac gtaggcacat tcgacaacgc cttgttcgcc ctgcagtgca cgaaccagag
    54241 acagaccaaa acgtgcagct gcctggccca tagacagggt tgcagaccca ccaccggctt
    54301 tcgcttcaac cacttcagta cctgcgttct ggatacgttt ggtcagatca gccacttcct
    54361 gctcggtaaa actaacgcca ggaacctgtg acagcagcgg cagaatggta acaccagagt
    54421 gaccgccaat aaccggcact tcaacttcgc ctggctgttt gcctttcagt tccgcaacaa
    54481 aagtgttgga acgaatgata tccagcgtgg taacgccgaa cagtttgttt ttgtcataaa
    54541 caccggcttt tttcagcact tcagcagcaa tcgcaactgt ggtgttaacc gggttagtga
    54601 taataccaat gcacgctttc gggcaggttt tcgcaacttg ctgtaccagg tttttcacga
    54661 tgccggcgtt aacgttaaac aggtcggaac gatccatacc cggtttacgc gctacacctg
    54721 cagagataag aacgacatct gcgccttcca gcgccggagt cgcatcttca ccagaaaaac
    54781 ctttgatttt cacagcagta gggatatggc tcagatcgac agccacaccg ggagtcactg
    54841 gagcgatatc atacagagag agttctgaac ctgaaggcag ttgggttttt aacagtagtg
    54901 caagcgcctg gccaataccg ccagcagcgc cgaggactgc gactttcatc ctaaactcct
    54961 tattatattg ataaactaag atatgttgct ccgctgccgc gaccttaatc cacaaaattg
    55021 ccgtttacaa tgaccacatc tcaagaatgt gtagtcacgc aagtttagcg tttatgcatt
    55081 taattgccgt aatcaggaac ctaacttacg taattaacag tcgcttatgc attagcgcaa
    55141 cattccaaca ggtggtgaca atatacccta ccgttcagcc aaaacaacat caatttgata
    55201 acaattaatt tacttttaag cagaatttgc atgccgtgac gcaggcatgt ttctcaataa
    55261 cgaaatttga taaaatcccg ctctttcata acattatttc agccttcttc agggctgact
    55321 gtttgcataa aaattcatct gtatgcacaa taatgttgta tcaaccacca tatcgggtga
    55381 cttatgcgaa gctcggctaa gcaagaagaa ctagttaaag catttaaagc attacttaaa
    55441 gaagagaaat ttagctccca gggcgaaatc gtcgccgcgt tgcaggagca aggctttgac
    55501 aatattaatc agtctaaagt ctcgcggatg ttgaccaagt ttggtgctgt acgtacacgc
    55561 aatgccaaaa tggaaatggt ttactgcctg ccagctgaac tgggtgtacc aaccacctcc
    55621 agtccattga aaaatctggt gctggatatc gactacaacg atgcagttgt cgtgattcat
    55681 accagccctg gcgcggcgca gttaattgct cgcctgctgg actcactggg caaagcagaa
    55741 ggtattctgg gcaccatcgc tggcgatgac accatcttta ccacccctgc taacggtttc
    55801 accgtcaaag acctgtacga agcaatttta gagctgttcg accaggagct ttaatctctg
    55861 ccccgtcgct tctggcgtcg gggaaaatgt tgcttatccc tctcattccc cttctttctc
    55921 ctgcgataca tttaacgaat agtgcgtttt accgcgacac atcattcaca aaatgaatac
    55981 atacgg
//
LOCUS       DAZFRN010000009        54536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFRN010000009 DAZFRN010000000
VERSION     DAZFRN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.009"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            298..384
                     /gene="leuL"
                     /locus_tag="ACHKUB_000540"
     CDS             298..384
                     /gene="leuL"
                     /locus_tag="ACHKUB_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO6653611.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            477..2048
                     /gene="leuA"
                     /locus_tag="ACHKUB_000541"
     CDS             477..2048
                     /gene="leuA"
                     /locus_tag="ACHKUB_000541"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO6653612.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2048..3139
                     /gene="leuB"
                     /locus_tag="ACHKUB_000542"
     CDS             2048..3139
                     /gene="leuB"
                     /locus_tag="ACHKUB_000542"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO6653613.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3142..4542
                     /gene="leuC"
                     /locus_tag="ACHKUB_000543"
     CDS             3142..4542
                     /gene="leuC"
                     /locus_tag="ACHKUB_000543"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO6653614.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4553..5158
                     /gene="leuD"
                     /locus_tag="ACHKUB_000544"
     CDS             4553..5158
                     /gene="leuD"
                     /locus_tag="ACHKUB_000544"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO6653615.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5207..6385)
                     /gene="setA"
                     /locus_tag="ACHKUB_000545"
     CDS             complement(5207..6385)
                     /gene="setA"
                     /locus_tag="ACHKUB_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO6653616.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6487..6618)
                     /gene="sgrT"
                     /locus_tag="ACHKUB_000546"
     CDS             complement(6487..6618)
                     /gene="sgrT"
                     /locus_tag="ACHKUB_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO6653617.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6707..8362
                     /gene="sgrR"
                     /locus_tag="ACHKUB_000547"
     CDS             6707..8362
                     /gene="sgrR"
                     /locus_tag="ACHKUB_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO6653618.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADATRWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8396..8495
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8526..9509
                     /gene="thiB"
                     /locus_tag="ACHKUB_000548"
     CDS             8526..9509
                     /gene="thiB"
                     /locus_tag="ACHKUB_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO6653619.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9485..11095
                     /gene="thiP"
                     /locus_tag="ACHKUB_000549"
     CDS             9485..11095
                     /gene="thiP"
                     /locus_tag="ACHKUB_000549"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO6653620.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDSA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11079..11777
                     /gene="thiQ"
                     /locus_tag="ACHKUB_000550"
     CDS             11079..11777
                     /gene="thiQ"
                     /locus_tag="ACHKUB_000550"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO6653621.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11891..12655)
                     /gene="yabI"
                     /locus_tag="ACHKUB_000551"
     CDS             complement(11891..12655)
                     /gene="yabI"
                     /locus_tag="ACHKUB_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6653622.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12741..13619)
                     /gene="araC"
                     /locus_tag="ACHKUB_000552"
     CDS             complement(12741..13619)
                     /gene="araC"
                     /locus_tag="ACHKUB_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO6653623.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13958..15658
                     /gene="araB"
                     /locus_tag="ACHKUB_000553"
     CDS             13958..15658
                     /gene="araB"
                     /locus_tag="ACHKUB_000553"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO6653624.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15669..17171
                     /gene="araA"
                     /locus_tag="ACHKUB_000554"
     CDS             15669..17171
                     /gene="araA"
                     /locus_tag="ACHKUB_000554"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO6653625.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17340..18035
                     /gene="araD"
                     /locus_tag="ACHKUB_000555"
     CDS             17340..18035
                     /gene="araD"
                     /locus_tag="ACHKUB_000555"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6653626.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18110..20461
                     /gene="polB"
                     /locus_tag="ACHKUB_000556"
     CDS             18110..20461
                     /gene="polB"
                     /locus_tag="ACHKUB_000556"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO6653627.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20626..23532
                     /gene="rapA"
                     /locus_tag="ACHKUB_000557"
     CDS             20626..23532
                     /gene="rapA"
                     /locus_tag="ACHKUB_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO6653628.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23544..24203
                     /gene="rluA"
                     /locus_tag="ACHKUB_000558"
     CDS             23544..24203
                     /gene="rluA"
                     /locus_tag="ACHKUB_000558"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO6653629.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24320..25135)
                     /gene="djlA"
                     /locus_tag="ACHKUB_000559"
     CDS             complement(24320..25135)
                     /gene="djlA"
                     /locus_tag="ACHKUB_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO6653630.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25390..27744
                     /gene="lptD"
                     /locus_tag="ACHKUB_000560"
     CDS             25390..27744
                     /gene="lptD"
                     /locus_tag="ACHKUB_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO6653631.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27797..29083
                     /gene="surA"
                     /locus_tag="ACHKUB_000561"
     CDS             27797..29083
                     /gene="surA"
                     /locus_tag="ACHKUB_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO6653632.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29083..30072
                     /gene="pdxA"
                     /locus_tag="ACHKUB_000562"
     CDS             29083..30072
                     /gene="pdxA"
                     /locus_tag="ACHKUB_000562"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO6653633.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30069..30890
                     /gene="rsmA"
                     /locus_tag="ACHKUB_000563"
     CDS             30069..30890
                     /gene="rsmA"
                     /locus_tag="ACHKUB_000563"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO6653634.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30893..31270
                     /gene="apaG"
                     /locus_tag="ACHKUB_000564"
     CDS             30893..31270
                     /gene="apaG"
                     /locus_tag="ACHKUB_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO6653635.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31277..32125
                     /gene="apaH"
                     /locus_tag="ACHKUB_000565"
     CDS             31277..32125
                     /gene="apaH"
                     /locus_tag="ACHKUB_000565"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO6653636.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32150..32749
                     /locus_tag="ACHKUB_000566"
     CDS             32150..32749
                     /locus_tag="ACHKUB_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO6653637.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32785..33252
                     /locus_tag="ACHKUB_000567"
                     /pseudo
     CDS             32785..33252
                     /locus_tag="ACHKUB_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33351..33530)
                     /locus_tag="ACHKUB_000568"
     CDS             complement(33351..33530)
                     /locus_tag="ACHKUB_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO6653638.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33551..34030)
                     /gene="folA"
                     /locus_tag="ACHKUB_000569"
     CDS             complement(33551..34030)
                     /gene="folA"
                     /locus_tag="ACHKUB_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO6653639.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34222..36084)
                     /gene="kefC"
                     /locus_tag="ACHKUB_000570"
     CDS             complement(34222..36084)
                     /gene="kefC"
                     /locus_tag="ACHKUB_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO6653640.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36077..36607)
                     /gene="kefF"
                     /locus_tag="ACHKUB_000571"
     CDS             complement(36077..36607)
                     /gene="kefF"
                     /locus_tag="ACHKUB_000571"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO6653641.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36715..38046)
                     /gene="yaaU"
                     /locus_tag="ACHKUB_000572"
     CDS             complement(36715..38046)
                     /gene="yaaU"
                     /locus_tag="ACHKUB_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6653642.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVVFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38105..38392)
                     /gene="fixX"
                     /locus_tag="ACHKUB_000573"
     CDS             complement(38105..38392)
                     /gene="fixX"
                     /locus_tag="ACHKUB_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO6653643.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38389..39675)
                     /gene="fixC"
                     /locus_tag="ACHKUB_000574"
     CDS             complement(38389..39675)
                     /gene="fixC"
                     /locus_tag="ACHKUB_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6653644.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39726..40667)
                     /gene="fixB"
                     /locus_tag="ACHKUB_000575"
     CDS             complement(39726..40667)
                     /gene="fixB"
                     /locus_tag="ACHKUB_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6653645.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40682..41452)
                     /gene="fixA"
                     /locus_tag="ACHKUB_000576"
     CDS             complement(40682..41452)
                     /gene="fixA"
                     /locus_tag="ACHKUB_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO6653646.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41926..43440
                     /gene="caiT"
                     /locus_tag="ACHKUB_000577"
     CDS             41926..43440
                     /gene="caiT"
                     /locus_tag="ACHKUB_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO6653647.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43471..44613
                     /gene="caiA"
                     /locus_tag="ACHKUB_000578"
     CDS             43471..44613
                     /gene="caiA"
                     /locus_tag="ACHKUB_000578"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO6653648.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFTECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44742..45959
                     /gene="caiB"
                     /locus_tag="ACHKUB_000579"
     CDS             44742..45959
                     /gene="caiB"
                     /locus_tag="ACHKUB_000579"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO6653649.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46033..47586
                     /gene="caiC"
                     /locus_tag="ACHKUB_000580"
     CDS             46033..47586
                     /gene="caiC"
                     /locus_tag="ACHKUB_000580"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO6653650.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVVNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47695..48480
                     /gene="caiD"
                     /locus_tag="ACHKUB_000581"
     CDS             47695..48480
                     /gene="caiD"
                     /locus_tag="ACHKUB_000581"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO6653651.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFDEKRDPVWKGR"
     gene            48486..49076
                     /gene="caiE"
                     /locus_tag="ACHKUB_000582"
     CDS             48486..49076
                     /gene="caiE"
                     /locus_tag="ACHKUB_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO6653652.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49162..49557)
                     /gene="caiF"
                     /locus_tag="ACHKUB_000583"
     CDS             complement(49162..49557)
                     /gene="caiF"
                     /locus_tag="ACHKUB_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO6653653.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49818..53039)
                     /gene="carB"
                     /locus_tag="ACHKUB_000584"
     CDS             complement(49818..53039)
                     /gene="carB"
                     /locus_tag="ACHKUB_000584"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO6653654.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53057..54205)
                     /gene="carA"
                     /locus_tag="ACHKUB_000585"
     CDS             complement(53057..54205)
                     /gene="carA"
                     /locus_tag="ACHKUB_000585"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO6653655.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13175 a        14724 c        14262 g        12375 t
ORIGIN      
        1 tcgcaaaaca tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg
       61 tccccttttt cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa
      121 aagcgtcggt agttaagcag aaattaatat cgcttacttt aaccaccgca gcacaattag
      181 ctaattttac ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca
      241 tccgtttttg tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg
      301 actcacatcg ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga
      361 cgagtgagcg gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg
      421 cgcgggtttt tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga
      481 gccagcaagt cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa
      541 gcttgagtgt gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg
      601 tgatggaagt cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg
      661 cccgccaggt taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggag aaagatatcg
      721 acgtggcggc cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcca
      781 cttcgccaat gcacatcgcc accaagctgc gcagcacgct ggatgaagtg atcgaacgcg
      841 ctatctatat ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag
      901 atgccgggcg tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg
      961 gtgccaccac catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg
     1021 gaatcatcag cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac
     1081 atacccacga cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg
     1141 cacgccaggt ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg
     1201 aagaagtcat catggcgatt aaagttcgta aggatattct caacgtccac accgccatta
     1261 atcaccagga gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc
     1321 cggcaaacaa agccattgtt ggcagcggcg cattcgcaca ctcctccggt atccaccagg
     1381 atggcgtgct gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga
     1441 accaaatcca gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg
     1501 atgagatggg gtataaagaa agtgaatata atttagacaa tttgtacgac gctttcctga
     1561 agctggcgga caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg
     1621 gtaagcagca agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct
     1681 ctaacgatat cgccaccgcc gccgtcaaac tggcctgcgg cgaagaagtc aaagcagaag
     1741 ccgccaacgg taacggtccg gtcgatgccg tctatcaggc gataaaccgc atcactgact
     1801 ataacgtcga actggtgaaa tacagcctga ctgctaaagg tcacggtaaa gatgctctgg
     1861 gtcaggtgga tattgtcgcc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca
     1921 ccgatattgt cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgcg
     1981 ccgcagaagt cgaaaaagag ttgcaacgca aagctcaaca caacgaaaac aacaaggaaa
     2041 ccgtgtgatg tcgaagaatt accatattgc cgtattgccg ggggacggta ttggtccgga
     2101 agtgatgacc caggcgctga aagtgctgga tgccgtgcgc aaccgctttg cgatgcgcat
     2161 caccaccagc cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc
     2221 gcctgcgacg gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg
     2281 tccgaaatgg gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgttgccttt
     2341 gcgtaagcac ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga
     2401 agcattctgt ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc tgtgcgtgcg
     2461 cgaactgacc ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata
     2521 tgaaaaagcg tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat
     2581 cgcgtttgaa tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt
     2641 gctgcaatcc tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga
     2701 tgtcgaactg gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc
     2761 acagtttgac gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc
     2821 aatgatcact ggctcgatgg ggatgttgcc ttccgccagc ctgaacgagc aaggttttgg
     2881 tctgtatgaa ccggcaggcg gctcagcacc agatatcgca ggcaaaaaca tcgccaaccc
     2941 gattgcgcaa attctgtcgc tggcactgct gctgcgctac agcctggatg ccgatgatgc
     3001 ggcttgcgcc attgaacgcg ccattaaccg cgcattagaa gaaggcattc gcaccgggga
     3061 tttagcccgt ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta
     3121 tgtggcagaa ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac
     3181 gtagtgtacg aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat
     3241 gaagtgacct caccgcaggc gtttgatggt ctgcgcgccc acggtcgccc ggtacgtcag
     3301 ccgggcaaaa ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat
     3361 gcctgtggtg aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt
     3421 ggcgtcgagc tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg
     3481 gaacagggcg tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc
     3541 cacggcgcgt ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg
     3601 gcaacacaaa ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa
     3661 gccgcgccgg gcattacagc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc
     3721 gcaggcggca ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg
     3781 gaaggtcgta tgaccctgtg caatatggca atcgaaatgg gcgcaaaagc cggtctggtt
     3841 gcaccggacg aaaccacctt taactatgtc aaaggccgtt tgcatgcgcc gaaaggcaaa
     3901 gatttcgacg acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc
     3961 gataccgttg tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat
     4021 ccaggccagg tgatttccgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg
     4081 gttgaacgcg cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggcattccg
     4141 ctgaccgaag tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa
     4201 gatttacgcg cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca
     4261 ctggtggttc ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa
     4321 atctttattg aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg
     4381 aacaacgacc gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa
     4441 ggccgccagg ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct
     4501 gctgtgaccg gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga
     4561 gaaatttatc aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga
     4621 tgcaatcatc ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct
     4681 gtttaacgac tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct
     4741 gaacttcccg cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg
     4801 ctcctcgcgt gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc
     4861 gccgagtttt gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa
     4921 attaagcgat gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca
     4981 tttcgacgtg gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac
     5041 catcgatgcc ttccgccgcc actgcatgat gaacggtctg gacagtattg ggcttacctt
     5101 gcagcacgac gacgccattg ccgcttatga agcaaaacaa cctgcgttta tgaattaatc
     5161 cccttgcccg gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa
     5221 cctttgcggt taaaaataat gcgacaacag aaataaccgc aattacccag tagacagcaa
     5281 agtgccccca actttgtgca attgctccct gaataacgcc agccagaatt accccggtag
     5341 aaatactgtt agtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa
     5401 accatagcat cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg
     5461 tcatcaacgc catacggcta tgaaagaaaa tcaatccggt gtaaaacagt actcctgccg
     5521 ccactgctat gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa
     5581 tcattgctgg tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg
     5641 gcaatcctaa ctcgctactg atccacaacg gcatatcaat aatgtacatg gtgttgcagg
     5701 tccacattaa cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagccacctt
     5761 gcattgataa agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg
     5821 caatcaatac cagactgagt gtgaatatcc cggcggcaat cgaaaacatc accgtaaagc
     5881 cgtaattcaa cgccagcata aaggccaacg gtggaccgat aacccatgcc agagaaagct
     5941 gcgcacgcat caccgagcta aacatcacca cttctcgcgc cgagttatcc gcatattccc
     6001 gcgccagagc aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac
     6061 aggtgataag cgtcagataa tgacgattaa atgcaaacaa tagcgcattg ccgatagcca
     6121 tcaaacagca aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca
     6181 accagaggct tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga
     6241 aaggttgcgc gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc
     6301 cggccacccc catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc
     6361 gagccatcgt cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa
     6421 ccagcaggta taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca
     6481 ggcaagtcaa ctttcagaat tgcggtcatc ccactgcatc agttcttcaa gcatggttaa
     6541 tcgttgtggg acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg
     6601 ctgataaaac tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat
     6661 atagggaaaa taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg
     6721 tctgcaacaa cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac
     6781 gctcaacgaa ctggcagcgt tattgagctg ctcgcgtcgt catatgcgca ccctgctcaa
     6841 caccatgcag gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgtg gtaaacgctc
     6901 gcgtctgaca ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct
     6961 ggagcaggat cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca
     7021 aatgctggtt tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct
     7081 ctactatcgt ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca
     7141 tattgcccgg caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga
     7201 agcagacatc gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg
     7261 tccaggagtc cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt
     7321 aaaacgaatc aatacgctgc cgctctattc gcatattgcg gacattgtgt cgccgacgcc
     7381 ctggacgctg gatatccatc tcacgcaacc ggaccgctgg ttaccgttac tgctgggaca
     7441 agttccggcg atgatcctgc cgcgcgaatg ggaaaccctc agtaactttg ccagccatcc
     7501 catcggcacc ggtccgtatg cggtgatccg caacaccacc aatcaactga aaattcaggc
     7561 attcgatgac ttcttcggtt accgggcatt aatcgatgaa gttaacgtct gggttctgcc
     7621 ggaaattgcc gacgagccag ctggaggtct gatgctaaaa ggtccacagg gtgaggaaaa
     7681 agagattgaa agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca
     7741 tcgcggggcg aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct
     7801 ggtctatttc gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc
     7861 ccgttggcac catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac
     7921 cctgaccttt tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat
     7981 tctggcaagt caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga
     8041 aggagagatc gagagcgata tctggcttaa cagcgccaac tttacgctgc cgctggattt
     8101 ttcgctgttc gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg
     8161 gcaagccgac gccacccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca
     8221 actggtcgcc agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca
     8281 acgcagtatg cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg
     8341 gtttgcgccg ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt
     8401 ctcaacgggg tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga
     8461 taacgccggc gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt
     8521 gcaaagtgtt aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta
     8581 aacccgttct gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa
     8641 agatcaaaaa agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag
     8701 atggcgtttc gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg
     8761 tgctggggct ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa
     8821 gcggtgtggc agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc
     8881 cgtttgatta cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc
     8941 aaagcctgaa agaattggtt gagagcgatc aaaactggcg ggtgatttat caggatccgc
     9001 gcaccagtac gccggggctg ggcctgctgt tgtggatgca aaaagtctat ggcgatgacg
     9061 ccccacaagc ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg
     9121 aagcctacgg cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc
     9181 cggcttatca cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag
     9241 gtcactatct gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg
     9301 cgcaaaaatt cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta
     9361 actggatgta tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac
     9421 cggccaccac gctggagttc acaccagctg aagtggctgc acaacgtcag gcatggatta
     9481 gcgaatggca acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc
     9541 cgccacgctg gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc
     9601 gcagggtaac tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc
     9661 cttctggcag gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg
     9721 cgccctctat cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac
     9781 cttgatcctc ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg
     9841 ctggctggca tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct
     9901 gcaaggcatt ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact
     9961 ccaggcactg gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg
    10021 cggctggcat tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt
    10081 tgccgcgctt atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg
    10141 cggtccgcag gcaactacta tcgagctggc tatttatcag gcgctgagtt acgactacga
    10201 tcctgcccgc gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct
    10261 gttgagtcag cgactaagta aggccattgc gcccggtacc acgctgctgc aaggctggcg
    10321 cgatccggac gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct
    10381 gctgctgttg ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc
    10441 ggaagtgctg gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct
    10501 ggcggcaggt gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact
    10561 gcgggcgcgg cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct
    10621 cgccatgccg gggattgtgc tggctaccgg cttcttttta ctgctcaaca acaccattgg
    10681 cctgccgcaa tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta
    10741 tgcgctgaaa gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg
    10801 tcagtcgctg ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa
    10861 acgtccactg gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt
    10921 agtggcgttg ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat
    10981 tggctcctat cgcagccagg acagtgcggt caccgcgtta attctgctac tactctgttt
    11041 tctgctgttt accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca
    11101 cctggcttta tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg
    11161 tggcaatcct cgggccaagc ggcgcgggta aaagtaccct gctgaatttg atcgccggtt
    11221 ttctgacgcc agccagcggt tcgctgacta tcgacggcgt tgatcacacc acgacgccgc
    11281 cgtcacgccg tccggtatcg atgctgtttc aggaaaacaa cctgttcagt cacctgacgg
    11341 tcgcgcagaa catcgggctg gggcttaatc cgggattgaa gctgaacgcg gcgcagcagg
    11401 agaaaatgca cgctatcgcc cgccagatgg ggattgataa tttaatggcg cggttaccgg
    11461 gcgagctttc cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac
    11521 agccgatttt attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga
    11581 tgctgacgct ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc
    11641 acagtgtgga agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca
    11701 tcgcctggca gggtaagacc aatgagttgt tgagcggtaa ggcgagtgct tcggcactat
    11761 tggggattac gggttagtat gcactgtcgg atgcggcgtg aacgccttat ccgacctacg
    11821 ggtagtgcgt tttgtaggcc tgataagacg cggtcagcgt cgcatcaggc aacccgcaca
    11881 agactatctc ctaaccccca accactttac gcaaaatatc gatatacacc ggcatcaacg
    11941 ggtggcgaat taacaccacc agcgccacca cgccgatggc agaaattaac ggcgtcagcc
    12001 acaacaaacg gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc
    12061 ataaccgcca gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc
    12121 atttaaactc gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc
    12181 caggcaggaa gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt
    12241 ttgccaccgg cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa
    12301 caaagcgacc gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat
    12361 cgagtaatgc tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc
    12421 agcccagcca gaaggagatc cagtcgccca gcaggcaacc gacaatccct gccagccagg
    12481 cgtgccagaa acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg
    12541 taccgggtag aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg
    12601 ccatcaatga atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt
    12661 gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca
    12721 attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca
    12781 caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa
    12841 tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg
    12901 ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc
    12961 cctaactgct ggcggaaaag atgtgacaga cgcgacggtg acaagcaaac atgctgtgcg
    13021 acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg
    13081 cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt
    13141 aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg
    13201 gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga
    13261 aagaaacccg tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc
    13321 ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga
    13381 tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc
    13441 tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc
    13501 agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc
    13561 accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata
    13621 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc
    13681 cgtcactgcg tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa
    13741 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat
    13801 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata
    13861 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
    13921 gtttctccat acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga
    13981 ttttggcagt gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc
    14041 caccagcgta gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa
    14101 ccagttccgt catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt
    14161 gcttgcagag cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac
    14221 cggctcgacg cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt
    14281 tgccgaaaac ccaaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc
    14341 ggaagagatt acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctacattgg
    14401 tggtatttat tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag
    14461 cgccgtggcg caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct
    14521 ttccggtacc acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc
    14581 tctgtggcac gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc
    14641 gatcctcaat cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc
    14701 ggtgggcacc ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat
    14761 ttccggcggc gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc
    14821 actggtaaaa gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt
    14881 tggcgagcgg gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt
    14941 tatcggtctg gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt tcggtcgcgt
    15001 actcagctgg ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa
    15061 cgccagccag aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct
    15121 ggatcacctg ccggtggtgc tcgactggtt taacggtcgt cgcacaccga acgctaacca
    15181 acgcctgaaa ggggtgatta ccgatcttaa cctcgctacc gacgctccgc tgctgttcgg
    15241 cggtttgatt gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca
    15301 ggggatcgcc gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat
    15361 tatgcaggcc tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg
    15421 ctgtgcgctc ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc
    15481 atcagcccag caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca
    15541 ggcacaacgc tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca
    15601 acactatctt ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg
    15661 acacgataat gacgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc
    15721 tgtatggccc ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc
    15781 tgaatacgga agcgaaactg ccctgcaaac tggtgttgaa accgctgggc accacgccgg
    15841 atgaaatcac cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg
    15901 tgtggctgca cactttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca
    15961 aaccgttgct gcaattccac acccagttta acgcggcgct gccgtgggac agtatcgata
    16021 tggactttat gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg
    16081 cgcgtatgcg tcagcaacat gccgtggtta ccggtcactg gcaggataaa caagcccatg
    16141 agcgtatcgg ctcctggatg cgccaggcgg tctctaaaca ggatacccgt catctgaaag
    16201 tctgccgttt tggcgataac atgcgtgaag tagcggtcac cgatggtgat aaagttgccg
    16261 cacagatcaa gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg
    16321 tgaactccat cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca
    16381 ccatgacgcc tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc
    16441 gtattgagct ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca
    16501 cctttgaaga tttgcacggt ctgaaacagc ttccaggtct ggccgtacag cgtctgatgc
    16561 agcagggtta cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca
    16621 tgaaggtgat gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc
    16681 acttcgagaa aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga
    16741 ttgccgtaga agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg
    16801 atcctgcccg actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg
    16861 atctcggcga tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact
    16921 ccctgccgaa actgccggtg gcgaatgcgc tgtggaaagc gcaaccggat ctgccaactg
    16981 cttccgaagc gtggatcctt gctggtggcg cgcaccatac cgtcttcagc catgcgctga
    17041 acctcaacga tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata
    17101 acgacacccg cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat
    17161 ttcgtcgcta agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat
    17221 ctggcctaca cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattgttttc
    17281 cccctcgccc ctttggggag agggccgggg tgaggggaaa cgacctctca ggagtcaaca
    17341 tgttagaaga tctcaaacgc caggtattag aagccaacct ggcgctgcca aaacacaacc
    17401 tggtcacgct cacatggggc aacgtcagcg ccgttgatcg cgagcgcggc gtctttgtga
    17461 tcaaaccttc cggcgtcgat tacagcgtca tgaccgctga cgatatggtc gtggttagca
    17521 tcgaaaccgg tgaagtggtt gaaggtacga aaaagccctc ctccgacacg ccaactcacc
    17581 ggctgctcta tcaggcattc ccctccattg gcggcattgt gcatacgcac tcgcgccacg
    17641 ccaccatctg ggcgcagtcg ggtcagtcga ttccagcaat cggcaccacc cacgccgact
    17701 atttctacgg caccattccc tgcacccgca aaatgaccga cgcagaaatc aacggcgaat
    17761 atgagtggga aaccggtaac gtcatcgtag aaacctttga aaaacagggt atcgatgcag
    17821 cgcaaatgcc cggcgttctg gtccattccc acggcccgtt tgcatggggc aaaaatgccg
    17881 aagatgcggt gcataacgcc atcgtgctgg aagaggtcgc ttatatgggg atattctgcc
    17941 gtcagttagc gccgcagtta ccggatatgc agcaaacgct gctggataaa cactatctgc
    18001 gtaagcatgg cgcgaaggca tattacgggc agtaatgact gtataaaacc acagccaatc
    18061 aaacgaaacc aggctatact caagcctggt tttttgatgg attttcagcg tggcgcaggc
    18121 aggttttatc ttaacccgac actggcggga caccccgcaa gggacagaag tctccttctg
    18181 gctggcgacg gacaacgggc cgttgcaggt tacgcttgca ccgcaagagt ccgtggcgtt
    18241 tattcccgcc gatcaggttc cccgcgctca gcatattttg cagggtgaac aaggctttcg
    18301 cctgacaccg ctggcgttaa aggattttca ccgccagccg gtgtatggcc tttactgtcg
    18361 cgcccatcgc caattgatga attacgaaaa gcgcctgcgt gaaggtggcg ttaccgtcta
    18421 cgaggccgat gtgcgcccgc cagaacgcta tctgatggag cggtttatca cctcaccggt
    18481 gtgggtcgag ggtgatatgc acaatggcac tatcgttaat gcccgtctga aaccgcatcc
    18541 cgactatcgt ccgccgctca agtgggtttc tatagatatt gaaaccaccc gccacggtga
    18601 gctgtactgc atcggcctgg aaggctgcgg gcagcgcatc gtttatatgc tggggccgga
    18661 gaatggcgac gcctcctcgc ttgatttcga actggaatac gtcgccagcc gcccgctgct
    18721 gctggaaaaa ctcaacgcct ggtttgcaaa ccacgatccc gatgtgatca tcggctggaa
    18781 cgtggtgcag ttcgatctgc gaatgctgca aaaacatgcc gagcgttacc gtcttccgct
    18841 gcgtcttggg cgcgataata gcgagctgga gtggcgcgag cacggcttta aaaacggcgt
    18901 cttttttgcc caggctaaag gtcggctaat tatcgacggt atcgaagcgc tgaaatccgc
    18961 gttctggaat ttctcttcat tctcgctgga aactgtcgct caggagctat taggcgaagg
    19021 aaaatctatc gataacccgt gggatcgaat ggacgaaatt gaccgccgtt tcgccgaaga
    19081 taaacctgcg ctggcaactt ataacctgaa agattgcgag ctggtgacgc agatcttcca
    19141 caaaactgaa atcatgccat ttttactcga acgagcaacg gtgaacggcc tgccggtgga
    19201 tcggcacggc ggttcggtgg cagcgtttgg tcatctctat tttccgcgaa tgcatcgcgc
    19261 tggttatgtc gcgcctaatc tcggcgaagt gccaccgcac gccagccctg gcggctacgt
    19321 gatggattca cggccagggc tttatgattc agtgctggtg ctggactata aaagcctgta
    19381 cccgtcgatc atccgcacct ttctgattga tcccgtcggg ctggttgaag gcatggcgca
    19441 acctgatcca gagcacagta ccgaaggttt tctcgatgcc tggttctcac gggagaaaca
    19501 ttgcctgccg gagattgtga ctaacatctg gcacgggcgc gatgaagcca aacgtcaggg
    19561 taataagccg ctttcgcagg cgctgaagat cattatgaat gccttttatg gcgtgctcgg
    19621 caccaccgcc tgccgcttct tcgatccgcg cctggcatcg tcgatcacca tgcgtggtca
    19681 tcagatcatg cggcagacca aagcattgat tgaagcgcag ggctacgacg ttatctacgg
    19741 agataccgac tcgacttttg tctggctaaa aggtgcacat tcggaagaag aggcggcgaa
    19801 aatcggtcgt gtactggtgc agcacgttaa cgcctggtgg gcggaaacgc tgcaaaaaca
    19861 acggctgacc agcgcattag aactggagta tgaaactcat ttctgccgtt ttctgatgcc
    19921 aaccattcgc ggagccgata ccggcagtaa aaagcgctac gccgggctga ttcaggaggg
    19981 cgataaacag cggatggtgt ttaaagggct ggaaaccgtg cgcaccgact ggacgccgct
    20041 ggcccagcag tttcagcagg agctatacct gcgcatcttc cgcaacgagc catatcagga
    20101 atatgtacgc gaaaccatcg acaaactgat ggcgggtgaa ctggatgcgc gactggttta
    20161 ccgtaaacgc cttcgccgtc cgctgagcga gtatcagcgt aatgtgccgc ctcatgtacg
    20221 cgccgctcgc cttgccgatg aagaaaacca aaagcgcggt cgccccttgc aatatcagaa
    20281 tcgcggcacc attaagtacg tatggaccac caacggcccg gagccgctgg actaccaacg
    20341 ttcaccactg gattacgaac actatctgac ccgccagcta caacccgtgg cggagggaat
    20401 actccctttt attgaggata attttgctac acttatgacc gggcaacttg ggctattttg
    20461 agcaaaaaaa agagttcgcc agataccatt ttgatgcgtg acgaatgctt tgccatccag
    20521 taccatagcg ccctttccat tcctggacct gaataacacc actacctcat aagcacggta
    20581 gcgggtggtt attgcctgca attaaagata tagagccgaa cacatatgcc ttttacactt
    20641 ggtcaacgct ggatcagcga tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg
    20701 gatgcgcgaa ctgtcacttt acttttccca tctactggtg aaaaccgtct gtacgcacgc
    20761 agtgattccc ccgtgacccg cgtgatgttc aaccctggtg atactattac cagccatgac
    20821 ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt
    20881 actcgcctgg atactgaaga gtccggcgta gccctgcgtg aagttttcct tgatagcaaa
    20941 ctggtgttca gcaaaccgca ggaccgtctg tttgccgggc agattgaccg tatggaccgc
    21001 tttgcgctgc gttatcgcgc gcgtaagtat tccagcgaac agttccgtat gccgtacagc
    21061 ggcctgcgcg gtcagcgtac cagcctgatt ccgcatcagc tcaacatcgc tcatgatgtt
    21121 ggtcgccgcc acgcgccgcg cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt
    21181 gaagccggga tgatcctgca tcagcaactg ctctctggcg ctgctgaacg tgtgctaatt
    21241 atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg
    21301 cgctttgcgc tatttgatga tgagcgttat gccgaagctc agcacgatgc ttacaacccg
    21361 tttgacaccg aacagctggt gatttgctcg ctggattttg cccgtcgtag caaacagcgc
    21421 ctggaacatc tctgtgaagc cgaatgggac ctgctggtgg tcgatgaagc gcatcacctg
    21481 gtgtggagcg aagatgcgcc aagccgtgaa tatcaggcca ttgaacaact ggcagagcac
    21541 gtgccgggcg ttctgctgct gaccgcgacc ccggaacagc tggggatgga aagccacttc
    21601 gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt ttgcgcagtt cgttgaagag
    21661 cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc tgctggcagg taacaaactg
    21721 agcaatgacg aactgaacat gctcggcgag atgatcggcg agcaggatat cgagccgctg
    21781 ttgcaggcag caaacagcga cagcgaagat gcccagagcg cccgtcagga gctggtttcg
    21841 atgctgatgg atcgccacgg caccagccgc gtgctgttcc gtaacacgcg taacggtgtg
    21901 aaaggattcc cgaaacgcga gctgcacacc attaaactgc cgctaccgac gcagtatcag
    21961 acggctatta aagtctccgg cattatgggc gcacgtaaaa gtgcggaaga tcgtgctcgc
    22021 gatatgctct acccggagcg tatttatcag gaatttgaag gtgataacgc cacctggtgg
    22081 aacttcgatc cgcgcgttga gtggctgatg ggctacctga ccagccatcg ctctcagaaa
    22141 gtgctggtga tctgcgccaa agctgccact gcgctgcaac tggagcaggt actgcgcgaa
    22201 cgtgaaggta ttcgcgctgc ggtgttccac gaaggtatgt cgattatcga acgtgaccgc
    22261 gctgccgcct ggtttgccga agaagacacc ggcgcacagg tactgctgtg ctcagaaatc
    22321 ggttctgaag gacgtaactt ccagttcgcc agccacatgg tgatgtttga cctgccattc
    22381 aacccggatc tactggagca gcgtattggt cgtctggatc gtatcggcca ggcgcacgat
    22441 attcagatcc atgtgcctta tctggagaaa accgctcagt cggtgctggt gcgctggtat
    22501 cacgaaggtc tggatgcatt tgagcacacc tgcccgaccg gacgcactat ttacgatagc
    22561 gtatacaacg atctgattaa ctatctggct tcaccggatc aaaccgaagg ctttgacgat
    22621 ctgatcaaaa actgccgcga gcaacatgaa gcgctgaaag cacagctgga acagggtcgt
    22681 gaccgcctgc tggaaatcca ctccaacggt ggcgaaaaag cccaggcact ggcagaaagc
    22741 attgaagagc aggatgacga taccaacctg atcgccttcg ccatgaacct gttcgatatt
    22801 atcggtatca atcaggacga tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat
    22861 atgctggtgc cggacttccc tggcctgtcg gaagatggca tcaccatcac ctttgatcgt
    22921 gaagtggcac tggcgcgtga agatgcacag tttattacct gggagcatcc gctgatccgc
    22981 aacggtctgg atctgatcct ttctggcgat accggtagca gcacgatttc actgttaaaa
    23041 aacaaagcgt tgccggtagg tacgctgttg gtggaactga tttatgtggt tgaagcccag
    23101 gctccgaagc agttgcagct caaccgcttc ctgccaccga cgccggtacg tatgctgctg
    23161 gataaaaacg gcaacaacct ggcggcgcag gtagagtttg aaacctttaa ccgccagctt
    23221 aacgcggtta accgtcacac cggcagcaaa ctggttaacg ccgtgcagca ggatgttcac
    23281 gctatccttc aactgggtga agcgcagatc gagaaatctg cccgtgcatt gattgatgca
    23341 gcgcgtaacg aagccgacga aaaactgtct gccgagctgt ctcgtctgga agcgctgcgc
    23401 gcagtgaacc cgaacattcg tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag
    23461 gtaatggaaa gcctggatca ggcaggctgg cgtctggatg ccctgcgttt gatcgttgta
    23521 acgcatcagt aacggagccg aaaatgggga tggaaaacta caatccaccg caggaaccct
    23581 ggttggttat cctgtatcag gatgaccaca ttatggtggt taacaagccg agcggtttgt
    23641 tgtctgtgcc gggtcgtctg gaagagcaca aagatagcgt gatgacgcgc attcagcgtg
    23701 attatccgca ggcagaatcg gtgcatcgtc tggatatggc taccagcggc gtgattgtag
    23761 tggcgctgac caaagccgcg gagcgggagt taaaacgcca gttccgcgag cgcgagccga
    23821 aaaagcagta tgtggcccgc gtctgggggc atccatcccc cgcagaaggt ctggtggatc
    23881 tgccgctgat ttgcgactgg ccaaaccgcc cgaaacagaa agtctgttac gaaacgggta
    23941 aacctgcgca gacggaatat gaagtggtgg agtatgcggc ggataacacg gcaagagtgg
    24001 tgttaaaacc gattaccggg cgttcgcatc aattgcgtgt gcatatgctg gcgctggggc
    24061 atccgattct cggcgatcgt ttttatgcat caccagaagc gagagcgatg gcaccgcgtt
    24121 tgttgctaca tgcagagatg ttgacgatta cccatccggc gtatggcaat agtatgacgt
    24181 ttaaagcgcc agcggatttt taatcgtcac gctggcgcag ttcggtaggc ggataaggcg
    24241 ttcacgccgc atccggcaat tgtgcgccga ttgcctgatg cgacgcgggc cgcgtcttat
    24301 caggcctaca gtttaaaact tatttaaacc ctttctgctg ctttatcagc tcatatgcct
    24361 gctgaatttc ctgcgctttc tgcttcgcca tctccatcat ctcaggcggc aaacctttcg
    24421 ccaccagctt atcgggatgg tgttcactca tcagcttacg gtaggcacgt ttgatggtgg
    24481 tcgcatcatc cgtcggcttc acgcccagca cattacaggc atcttccagc gtcgggccac
    24541 gctgcgcttg ctgccagtta ccaccgccag attgctgctg ataaccgccg ccaaactgtg
    24601 caccgccctg catcatgcgc aaaaactggt caaactgggc gcgggagatc cctaattctt
    24661 cagcaatgac atacagcacc gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg
    24721 cctgaatctg gatctccaga aacatacgaa ttaagtcaaa acgaccaaag cagacactgc
    24781 gaaactggcg catcttttcg cgcagcgggt aattgtctga ttttcccacc cggaacgcat
    24841 tttgcgccgc agtacgggaa gcgccatgaa gattcattcg gtccatcaac tggctggcga
    24901 tatgaatatc agcctccgtg acgcgacctt tggatttggt taaatgcccc atcacttcaa
    24961 aagtggtggc aaaaaacagc gcctgacgct cacgctggtt ggcgaaccac gccattttac
    25021 ggctacgggc tttatcaaac atatggccaa ttaacaggcc taacactacg ccccaaaagc
    25081 cgccgcccat cagtaaggcc acggccacgc caatgatttt tccccaatac tgcatatatt
    25141 ccccaaatcg acacacggat atcagggcta tctcccacaa tataaaggtg cttttaccgt
    25201 tttccggctg cggtcaattg tgggacatcg cctataattt gcattatcat acctgtcatt
    25261 caatgccgtg cctaacacca ctgacgctat tcggacagga ttaacactag cgtagagatg
    25321 acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa
    25381 aatcaacgta tgaaaaaacg tatccccact ctcctggcca ccatgattgc caccgccctt
    25441 tatagtcaac agggactggc agccgacctc gcctcacagt gcatgttggg cgtgccaagc
    25501 tatgaccgtc ctctggtaca gggcgatacc aatgacttac ccgtgactat caatgctgac
    25561 cacgcgaaag gggactaccc ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag
    25621 ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc aaaaagaggc accaggacaa
    25681 ccggagccgg tacgtaccgt tgatgcgctc ggtaatgtcc attacgacga taaccaggtg
    25741 atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca aagataccaa cgtctgggaa
    25801 ggtgattacc agatggtggg tcgccagggt cgcggtaaag cggacctgat gaaacaacgt
    25861 ggcgaaaacc gctataccat tctggataac ggtagcttta cctcctgtct gccgggttct
    25921 gacacctgga gcgtggtagg tagcgaaatt attcatgacc gcgaagaaca agttgcggag
    25981 atctggaacg cccgctttaa ggtgggtccg gtaccgatct tttatagccc ctatttgcag
    26041 ttgccggtgg gtgacaaacg tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc
    26101 accaactact ttgagttcta cctgccatat tactggaaca tcgcgccaaa tatggatgcc
    26161 accatcacgc cgcattatat gcatcgtcgt ggcaacatca tgtgggagaa cgaattccgc
    26221 tacctctccc aggcgggcgc tggcttgatg gaactggact atctgccttc agataaagtc
    26281 tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt ggttattcta ctggaaccac
    26341 tccggggtca tggatcaagt gtggcgtttc aacgtcgact acaccaaggt cagcgatcct
    26401 agctacttca atgatttcga taacaagtac ggttccagta ctgacggcta cgcaacgcaa
    26461 aaattcagcg ttggctatgc ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc
    26521 caggttttca gcgaacagaa caccagtagc tactcggcag agccgcagtt agacgttaat
    26581 tactaccaga atgatgttgg tccgtttgat acgcgtattt acggccaggc agtgcacttt
    26641 gttaacacca gagacgacat gcctgaagca acccgtgttc acctggaacc gaccatcaat
    26701 ttgccgctct ctaataactg gggcagcatc aataccgaag cgaagttgct ggcaacccat
    26761 tatcagcaaa ccaatcttga ctggtataac tccagaaaca cgaccaagct ggacgaatcc
    26821 gttaaccgcg taatgccgca attcaaagtt gacggcaaaa tggtctttga acgcgatatg
    26881 gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc gcgcgcagta tttgtacgtg
    26941 ccgtatcgcg atcagagcga catctataac tacgactcgt ctctgctgca atctgactac
    27001 tctggcctgt tccgggaccg gacttacggc ggtcttgacc gtattgcctc cgctaaccag
    27061 gtgacgaccg gtgtcacatc tcgcatatat gatgatgctg ccgttgaacg ttttaatatt
    27121 tccgttggtc aaatctacta tttcacggag tctcgcactg gcgatgacaa cataacatgg
    27181 gagaatgacg acaaaacggg ttcactggtg tgggcaggcg atacttactg gcgtatctcc
    27241 gagcgttggg gattgcgtgg cgggattcag tacgatacac gtctggataa cgtagcgacc
    27301 agtaactcca gcattgaata ccgtcgggat gaagaccgtc tggtacagct gaattaccgt
    27361 tacgccagcc cggaatatat tcaggctacg ctgcctaagt actattccac tgctgagcaa
    27421 tataagaatg gtatttcgca ggtaggtgct gtcgccagct ggccaattgc cgatcgttgg
    27481 tccattgttg gggcctacta ctacgacacc aatgcgaaca agcaagccga ctctatgtta
    27541 ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg gttacgagcg gaagctgaac
    27601 ggttgggata acgataaaca acatgcggta tatgacaacg caatcggctt taacatcgaa
    27661 cttcgcggcc tgagctccaa ctacggtctg ggtacgcaag agatgctgcg ttcgaacatt
    27721 ctgccgtatc aaaacacttt gtgatttgtt gatttaccac gtaatccgca gtgcggttaa
    27781 ttgaaatgga aaaagtatga agaactggaa aacgctgctt ctcggtatcg ccatgatcgc
    27841 gaataccagt ttcgctgccc cccaggtagt cgataaagtc gcagccgtcg tcaataacgg
    27901 cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg gtaaaactga acgctgctca
    27961 ggcaaggcag caacttcctg atgacgcgac gctgcgccac caaatcatgg aacgtttgat
    28021 catggatcaa atcatcctgc agatggggca gaaaatggga gtgaaaatct ccgatgagca
    28081 gctggatcag gcgattgcta acatcgcgaa acagaacaac atgacgctgg atcagatgcg
    28141 cagccgtctg gcttacgatg gactgaacta caacacctat cgtaaccaga tccgcaaaga
    28201 gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt cgcatcacca tcctgccgca
    28261 ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac gacgccagca ctgagctgaa
    28321 cctgagccac atcctgatcc cgctgccgga aaacccgacc tctgatcagg tgaacgaagc
    28381 ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagct
    28441 ggcgattgct cattctgccg accagcaggc gctgaacggc ggccagatgg gctggggccg
    28501 tattcaggag ttgcccggga tcttcgccca ggcattaagc accgcgaaga aaggcgacat
    28561 tgttggcccg attcgttccg gcgttggctt ccatattctg aaagttaacg acctgcgcgg
    28621 cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc
    28681 gccgatcatg actgacgaac aggcccgtgt gaaactggaa cagattgctg ctgatatcaa
    28741 gagtggtaaa acgacttttg ctgccgcagc gaaagagttc tctcaggatc caggctctgc
    28801 taaccagggc ggtgatctcg gctgggctac accagatatt ttcgatccgg ccttccgtga
    28861 cgccctgact cgcctgaaca aaggtcaaat gagtgcaccg gttcactctt cattcggctg
    28921 gcatttaatc gaactgctgg atacccgtaa tgtcgataaa accgacgctg cgcagaaaga
    28981 tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca
    29041 ggaacaacgt gccagcgcct acgttaaaat cctgagcaac taatggttaa aacccaacgt
    29101 gttgtgatca ctcccggcga gcccgccggg attggcccgg acttagttgt ccagcttgca
    29161 cagcgtgagt ggccggtcga actggttgtt tgtgccgatg ccactctcct taccgaccgg
    29221 gcagcgatgc tcggtttgcc gctcaccctc cgcccttatt cccccaactc ccctgcacaa
    29281 ccgcaaacta cgggcacatt aacgctactt cctgtcgcgc tacgtgaatc tgtcactgcg
    29341 gggcagttag cgattgaaaa tggacattac gtggtggaga cgctggcgcg agcgtgtgat
    29401 ggctgtctga acggtgaatt tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt
    29461 aacgacgcag gcattccgtt taccggtcat accgagtttt tcgaagagcg ttcgcaggcg
    29521 aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat
    29581 ttaccgctgc gcgatatcgc agatgctatc acccctgcac ttttgcacga agtgattgct
    29641 attttgcatc acgatttgcg tacaaaattt ggtattgccg aaccgcgcat tctggtctgc
    29701 gggctgaatc cgcacgcggg cgaaggcggt catatgggta cggaagagat agacaccatt
    29761 attccggtgc tcgacgagct gcgggcgcag gggatgaaac tcaacgggcc gctgcctgcc
    29821 gataccctgt ttcagccgaa atatctcgat aacgccgacg ccgtgctggc gatgtaccac
    29881 gatcagggtc ttcccgtgct aaaataccag ggcttcgggc gcggtgtgaa cattacgctg
    29941 ggcctgccct ttattcgcac atcagtggac cacggcaccg cgcttgaact ggcgggacgt
    30001 ggcgaagccg atgtcggcag ttttattacg gcgcttaatc tcgccatcaa aatgattgtt
    30061 aacacccaat gaataatcga gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa
    30121 actttctcaa cgatcagttc gtgatcgaca gcattgtttc tgccattaac ccgcaaaagg
    30181 gccaggcaat ggtcgaaatc ggccccggtc tggcggcatt gaccgagccg gtcggcgaac
    30241 gtctggacca gctgacggtc atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc
    30301 atccattctt aggcccgaaa ctgacgattt atcagcagga tgcgatgacc tttaactttg
    30361 gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca
    30421 tctccacgcc gttgatgttc catctgttta gctatactga tgccattgcc gacatgcact
    30481 ttatgttgca aaaagaggtg gtgaatcgtc tagttgcagg accgaacagc aaagcgtatg
    30541 gtcgattaag cgtcatggcg caatactatt gcaacgtgat cccggtactg gaagttccgc
    30601 cgtcagcctt tacaccacca cccaaagtgg attccgccgt cgtgcgcctg gttcctcatg
    30661 caacgatgcc tcacccggtt aaagatgttc gtgtgttgag ccgcatcacc accgaagcct
    30721 ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt
    30781 taacgggaat ggggatcgac ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt
    30841 gccagatggc gaactatctg gcggagaacg cgcctttgca ggagagttaa cgatgatcaa
    30901 ttcgccccga gtgtgtattc aggttcaaag cgtctacatt gaggcacaat cttcacctga
    30961 taatgaacgt tacgtttttg cttataccgt aaccatacgc aatctggggc gagcgccagt
    31021 gcagttgttg gggcgttact ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca
    31081 gggcgaagga gtggttggcg tccagccact tatcgcgcct ggcgaagagt accagtacac
    31141 cagcggtgca atcattgaaa ccccgctggg caccatgcag ggtcactacg aaatgatcga
    31201 tgaaaatggc gtccctttca gcatcgacat tcccgtattc cgactcgccg ttcccacact
    31261 cattcattaa aagaatatgg cgacatacct tattggcgac gttcatggtt gttacgatga
    31321 actgatcgca ttgctgcata aagtagaatt tacccctggg aaagataccc tctggctgac
    31381 gggcgatctg gtcgcgcgcg ggccgggttc gctggatgtt ctgcgctatg tgaaatcctt
    31441 aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg catctgctgg cggtatttgc
    31501 cgggatcagc cgcaataaac cgaaagatcg cctgacaccg ctgctggaag cgccggatgc
    31561 cgacgagctg cttaactggc tgcgtcgcca gcctttgctg caaatcgacg aagagaaaaa
    31621 gttggtgatg gcccacgcag ggatcacgcc gcagtgggat ctacagaccg ccaaagagtg
    31681 cgcgcgcgat gtagaagcgg tgctatcgag tgactcctat cccttctttc tcgacgctat
    31741 gtacggcgat atgccaaata actggtcacc ggaattgcgg gggctgggaa gactgcgttt
    31801 tatcaccaac gcctttaccc gtatgcgttt ttgctttcca aacggtcaac tggatatgta
    31861 cagcaaagaa tcgccggaag aagcccctgc cccactgaaa ccgtggtttg cgattcctgg
    31921 ccctgtcgct gaagaataca gcatcgcctt tggtcactgg gcatcgctgg aaggcaaagg
    31981 tacgccggaa ggtatttacg cgctggatac cggctgctgc tggggtgggt cattaacctg
    32041 cctgcgctgg gaagataagc agtattttgt ccagccgtcg aaccggcata aggatttggg
    32101 tgagggagag gcggtcgcgt cttaacgaat taccattcag ggaggaataa tggctgacta
    32161 ttttgaaata cgtgctgatt acaatcagca caccatcacg atttatcagg cttacaatga
    32221 tgcaatcgct gacgtcgccg tacgtgacgg gcgatttggc gcaccttttt cattcaaccg
    32281 catgacctgg attaaaccct cttttatgtg gatgatggag cgtagcaact ggggactgaa
    32341 aaaagatcaa caacacattc tcgcaatccg aatcaaacgc accttctttg atacattgct
    32401 ggaacaagcg gtgctaacca cgccggaagc ccatgtgtat ccacatgcag gaatttggga
    32461 aacgttattt gcacaagcaa atgtgtatgt tcagtgggac ccagaacgca gcataaatgg
    32521 taaaaaactg gagcaccgat cattacagct tggtatttct cgtaacttga tcagtcaatt
    32581 taacgaagac gcgattgttg ctattgatga tctgacgccg ctggtgcgaa agtgtcataa
    32641 ccttttgata aacggcaaaa caacacaggc gaaatccttc ctgccgccag aaaaaattta
    32701 cccagttagt gcagcagcga gaaaggcttt gggaatgaaa aattcctgag acaaatacgc
    32761 acctattaag cggaaaatat ctaagtgcga caaatcaaca ccgggcttaa taccagagaa
    32821 attattctgg aaatgattga cgaagcagat gaccccccgt ttgctgagca gcggacctgg
    32881 catcgcaggc gaatcgcttt tctatgtgat tgcatcagcg ttgctgtcga ctacggcttc
    32941 ttctgttctg tctctcagca gtcaataatg aacataacca agatggctga agatgtatgg
    33001 ataacagaaa gtaaaacgac ccaactcatt gatatttgac aacaacttaa acagctttat
    33061 ccgccagaaa ctattcccta cgcccagaag ttacccacta aggaattgct tgaactgcta
    33121 ctttctagtc ctgacgatct tcgaataatt gaaattcctg acatcatcgg tgatgttttt
    33181 gatgaccaaa cctggattcg attattcaaa aaacactttg ctgatgttat tcagctacga
    33241 tgtgatgtgt aacaatccaa aatatatcgc cagacgatag accaaaaaga aagtatttag
    33301 cagatactca acattctgac ttctgtacag aaatagcaga gtttaaaaac ttaaaacttc
    33361 cggtactcat cagagaaaca tccagtttct tctgcaaatt catttaatgc cgagatggca
    33421 tcctcgttta cttcattcca ctgtagagtg gcttgaaggt ttagacctac gcaatgagcg
    33481 cgactcacaa aatcgcgctc actggtaaaa tcaaacgtct tatcgttcaa tttgtagtcc
    33541 tggcaaaaaa ttaccgccgc tccagaatct caaagcaata gctgtgagag ttctgcgcat
    33601 cggcgtcgtg gaattcgctg aataccgatt cccagtcatc cggctcgtaa tccgggaaat
    33661 gggtgtcgcc ttccacttct gcgtcgatat gcgtcagata cagtttttgc gcttttggca
    33721 ggaactgctc ataaacgcga ccgccgccaa tcaccatgat ttctggtacg tcaccacacg
    33781 ccgcgatggc ttcatccacc gacttcaccc acgttacgcg atcgtccgta cccggttgac
    33841 tgctgaggat aatatttttg cgtcctggca acggacgacc gattgattcc caggtatggc
    33901 ggcccataat cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca
    33961 ggttccacgg catggcgttt tccatgccga taacgcgatc taccgctaac gccgcaatca
    34021 gactgatcat tgagatttcc cgataaaaaa aattgtcgcc actatacgta aagcgtaaac
    34081 cgtcgtcgac tggtgcgagg atgatgttga ggaaaatttt atattctgct ggcgagtcca
    34141 cgctctctcc ctggactcgc cgcattacaa tgaaacaaaa acaaatagtt agctgtaaag
    34201 tgtgatttac gtcactcttt attaggatga gggtttcgtt tccggttcat ccgccatgtt
    34261 gccggtatgt ttaccttctt cggttccctg ccagccatgt cgttgaatta atgacagatg
    34321 ttcgcggtcc tcggtaatga tctcacttaa catcgcgctg gtgcgtttat agaccgccgc
    34381 gcgggctttg gtgtcgttct caaccattgc catctcttcc accatctgaa tattaaagcg
    34441 gcggaacaca tcggcacgtt ctcgcgcttc atacggcccc agacctaaac tttccagtgc
    34501 cagacgcccg gttttcagcg caccttcgaa ggtttcacgc tccggctttt caacgcctgc
    34561 ctgacgcaaa cgaatgtagt ggtcgacatc gcgggcgcgg gcaataatct gcaaatgcgg
    34621 gaaatgttct ttcaccatct ctgtcagttg caggttggtt tgcggatcgt cgatggcgtt
    34681 aatcagcact tccgctttcg ccgctccggc agattccagt aaatccatcc gcgtggcatc
    34741 gccataaaac actttcatac caaatttacg caaggtttcg atatggtccg gatcgtgatc
    34801 gagtaccacc attttcaccc cgctggagag cagtaaacgt ccggtaatct gcccaaaacg
    34861 accgaatccg gcgataatca cgcgcggctg ttcttcgtcg atctcatcgg cttcacgcgc
    34921 ttcctcagta gaagattgct caaggcgatt gaggatcacc agcagaatcg gcgttgctgc
    34981 catcgacagc gccaccgcca gggtcagcga tttcgcccac tccggctcca gcacattcgc
    35041 catctgcgcc gcgccaaata ccacaaaggc aaactcactg ccctgcccta acaacaccgc
    35101 aaaccaacga cgctgtttat ttggcacttg caacggtcgg gcaatcagcc acagcatggc
    35161 gattttgatg atgaggaaac cgagcagcaa aatgacaatg cgcaatgggt tttcaagcag
    35221 cgtgccaaag tctatcgaca tgccaacacc gatgaaaaac agccccaaca gcaaaccttt
    35281 aaatggttcg atatcgctct ccagcgcatg acggtattcc gaacttgcca gcagtacgcc
    35341 cgccagaaac gcgcccatcg ccatcgacaa gccgacctct tccagcagca aaccaaagcc
    35401 aaacacgagg aataacgcca cggcactaaa cacttcccgc aagccagagc gggctacaaa
    35461 acgcagcgcc ggacgcgtga catagcgccc cagcaatacc accagcacca gcgcacccgc
    35521 cacttttaac gccgagagag caaatgcgcc catcgtcgtc gaggcactgc tcgttgccag
    35581 tagcggaatc atcgccacca gcgggatcgc cgcgatatcc tggaacagca gcaccgcaaa
    35641 ggcactgcga cccatttgcg tcaccatcag attgcgttca ttcatcgcct gcatggcaat
    35701 cgccgtagag gagagcgcca gcgtcatgcc aatcaattcc gcgacctgcc agcgcagccc
    35761 aagtaacatg cagaacagcc ccagcaggcc gccgcaaatc accatctgca atgcgccacc
    35821 gccgaacact gccgcacgca gcttccacag cctttgtgga tcgagttcga ggccgataat
    35881 aaacagcatc agcaccaccc caatctcggc aaagtgcaga atagattcgg catcggtcac
    35941 cagtcgcagc ccccacgggc caataatgca gccggcgatc aggtagccaa gtaccgatcc
    36001 cagaccaaga cgtaccgcaa tgggtacaat cagcgctgcc gaaccgagat aaatcagcgc
    36061 ctgaaccagc gtatggctat ccatgatggg cctcctgcca ttccagcaga cgttgcttat
    36121 agtgacgcgc ctgcccttcg agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa
    36181 acggtggcag ccagttcagc ccgcagtaga ttgccgtcgc ctgtagcggc tgcgacagca
    36241 catcaaagcc cggatgcgca ccaatttcaa aatggctttc cccgccgccg gtcgtcaccg
    36301 cccacagcaa atgtttgcca tgcagcgccg tgccgccatg accgtaagcc cagccgtggg
    36361 agaaaacttt atcgatccaa agtttgagga gcggaggaat gctgtaccac tgcatcgggt
    36421 gctgccagac gatcagatcg gcgcgagaca gcgcctcctg ctcggcggca atatcgatat
    36481 tgaagtcagg atagagttga taaagagagc gaatttcgac gccttccagc gtccttgcct
    36541 gttcaagcat ccgtttattc gcatgggaat gatgcggata cggatgcgca taaattataa
    36601 gaatcatagg tagcccgcgt gattacgcct ttgttttccc acaaagtgta gtcatatatt
    36661 cacgcggctt atagtcaata atattgattc agaaaagaga gaaatctgaa caatttaccc
    36721 cattctctgc ccgcggatcg tcatattgct ggtttgcgcc agtgacatcc ctcgagtctc
    36781 cggggcaaac actacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat
    36841 cgtgttactg ataccgtaat tattgataaa gatcggcagt gcccaggtcg aaacaatggt
    36901 gccaatacgg cttaaggaca taatcacgcc cacggcagag gcgcggatat ctgtcgggaa
    36961 gagttcatta ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac
    37021 cgcaaaggcc atcactacca gccagatccc catatccggg atcagcccca aaaccgccag
    37081 cgccagcgtc atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag
    37141 ccacagcatc ggcggaatac agccgagcat aaagaacagg ctaatcacca cgttccccag
    37201 tgccgcgttt ttgccaaccc ccaatcccaa caaaccaacg atttgcgggc caaaggtgta
    37261 aatggcgaac attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg
    37321 aaaatggcgg cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa
    37381 agccaccggt tcgccaaaca gtttgatcat catctcttcg cactctttta ctcgcccttt
    37441 gcgtaataac cagcggggag attcaggcag ttcgaatcga ccaatcaaaa tcaacaaaca
    37501 ggggatcgcc gcgctaccca gcatccagcg ccagccgcct tccacatcat aaagccagta
    37561 gccgaccaga tcggcgcagg tcgcgccgac ataccacatg gcggcgataa agctgatgga
    37621 aaacgcccgc tgacgggtac tggagaactc ggtgatcatt gaggtggcga tgggataatc
    37681 tgcaccgatg acaatgccga taagtacccg catcaccaac agttcgacgg gggatgaaac
    37741 aaacatcgtc gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg
    37801 ccgtccgact ttatcggaaa tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag
    37861 cgttcccgcg cccaacaagc caatccagtc agcgtccagt tttagcgccg gagtcagttg
    37921 ctccagcgcc acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc
    37981 ccacagcaaa atgcggcggt gaattgagga gaatttgaga tcgtcaaagt ttctggacgg
    38041 ttgcatgggt tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc
    38101 aacatcagcc gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca
    38161 gcgccgaccc cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga
    38221 agcgcacact gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca
    38281 gctccagggc ctgtttatcg gcatcggcct tcacaacaat gtgcggatgc tcttcatcga
    38341 cattgaattt attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt
    38401 cactcctttc atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag
    38461 gattttcttg cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac
    38521 gcccaccgcc agttccgggt agccgctaaa catgcgtggg ttatcaagga aggccggtag
    38581 tttctggtac atacgcatat cgcgcagcgg gccactctca agatgctgac gatattccgc
    38641 cagtttttgc ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcggc
    38701 ttccccggcg gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat
    38761 tccggcggca tcaccggcaa tcaatacgcc gtcaccaacc aactccggca gcatgttgat
    38821 gcctgcttcc ggcactacgt gagcggaata ttccaccagc ttgccacccg cgatcagcgg
    38881 tgcaacggcc ggatgttgtt tgaaatcttc cagcatttgc ggcaccgatt tttttgcgtc
    38941 atgcagatga tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag
    39001 gaagccgccg cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg
    39061 attaccctgc aactgaaaac ggtcttcaat taccgacttc ggtaactcga tcagttcctt
    39121 cacgccaacc gccacatccg tcggtttaac gcgttttgcc atccccagct tttcggcaag
    39181 gatggagttc actccatcag caaggatcac cgttttcgct tcaatcacat cgccatcggc
    39241 ttctacaccg acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat
    39301 taactgcgcg cccgcttctt cggcctgctc catcagccag gcatcaaatt tactgcgcaa
    39361 aacggagtaa gaacgctggg atagcgaggt ttcgtcaccg ttgcagtagt ccatagtcat
    39421 cgctgacttt tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctaccggggc
    39481 ggattcggcg aaaccaggaa taatgtgttc caggctgtgg gcatagagac gcccgccggt
    39541 gacgttcttg gcacctgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg
    39601 ggcgagcacc agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa
    39661 gatatcttcg gacataagag ctccctgtca gcggcaggac caaaatgcag ccctgccaga
    39721 gtggatcaac gcgctaaagc tgcggtcagc gccggaagga tcttcacggc gtcgccaaca
    39781 atgccgtaat ccgcgtactg gaagatcggc gcatttttat ctttgttgat ggcgaaaatg
    39841 gtttgcgatg cattagcacc aaccatgtgc tggatctgcc cggagatccc caccgccagg
    39901 tacagttcag gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat
    39961 ttttcgtttt ccgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc
    40021 tgttctgcca gcgcaatgtt ctctttgctg ccaataccgc gaccgacgct gaccaccaga
    40081 cgggctttgt cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc
    40141 gccacagccg gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc
    40201 tgagccgcat cgaacgtacc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt
    40261 tcttcgccaa tcgccagacc accgtaaacc atgtgtttca ctgtcgcttt accgtcctgt
    40321 acgctgacgg tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc
    40381 gccagtaatt tgccgcgacg ggtgtttggc agcagaacca ggccgtctgc gccgtgctgg
    40441 cgaatagtgt cagccatgac actggcgtaa tcttcgatca tccgatcgtc cggtttgccg
    40501 tttaatttcc agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg
    40561 aggacaaagg cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga
    40621 cgagaagggg tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta
    40681 attaaatgac tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt
    40741 caatcacgat gcgctgacgc tcgcgctgtt tcggcgcagc aacctgttgt tcagaccagg
    40801 cgtccacggc gttaaaaccg atatccgccg ccgaccatac ctggactggc tttttcgccg
    40861 cgccgagaat ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa
    40921 caaccgcagg cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg
    40981 tgagggtatc tgcagtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga
    41041 ggatttcgcc caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac
    41101 agaggatcaa atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt
    41161 gcggcagtgc ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg
    41221 atagcacatc tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggccg
    41281 tcacctgcgc ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttcagat
    41341 cgtattggct tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga
    41401 ccgcaatatc ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc
    41461 cagaaatcat gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct
    41521 ttaatttgga aataaaactt taatcaccaa tattgaaaat gtcacacgca gataaatcca
    41581 actttcaata ttgttaagtt cctcaccaat attgaaaaca tggcgtagca aaaagaaatt
    41641 ttcaatattg ctttatggat caccaatatt gaaagtcaga tgctattttt ttactcacaa
    41701 cacaacataa caaactgatt aataatcaaa attaacgaga aaagcgccct ctattcaccc
    41761 gaaacaaaaa tgtgataaca atcacagaat gcagcttatt gaatacccat tatgagttag
    41821 ccattaacgc gtccacgagg ttaataataa ttatattaaa tgttaacaaa aataaaacaa
    41881 acgggaacgc aaaataaata ttcgttttca cagtggaatt aactcatgaa gaatgaaaag
    41941 agaaaaacgg gaatagaacc gaaggttttc tttccgccgt taataatcgt cggcatactt
    42001 tgttggctta cagtcagaga tctggatgca gcgaatgtcg ttattaatgc tgtattcagt
    42061 tacgtcacca atgtatgggg atgggcattt gaatggtata tggtggtgat gcttttcggt
    42121 tggttctggc tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga accacctgaa
    42181 tttagtaccg ccagttggat ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg
    42241 ttctggggat cgattgagat ctactactac atctccaccc cgccgtttgg cttagaaccg
    42301 aactcgacag gggcgaaaga gttggggctg gcttacagct tgttccactg gggacctctg
    42361 ccgtgggcca cttacagctt cctttcagtc gccttcgctt acttcttctt tgtccgcaaa
    42421 atggaagtga ttcgccccag ctcgacactg gtgccgctgg taggtgaaaa acacgccaaa
    42481 gggttgttcg gcactatcgt cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt
    42541 accagtctgg gccttgccac gccgctggtg accgagtgta tgcaatggtt gtttggcatt
    42601 ccgcataccc tgcaactgga cgctatcatc attacctgct ggattatcct caacgccatt
    42661 tgcgtcgctt gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg
    42721 agcttcctga tgctgggttg ggtgttcatt gtcagcggtg ccagcttcat catgaactac
    42781 ttcaccgatt cggtggggat gttgctgatg tatctgccgc gcatgttgtt ctataccgat
    42841 cccatcgcta aaggcggctt cccgcagggc tggaccgtgt tctactgggc atggtgggtg
    42901 atttatgcta tccagatgag tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt
    42961 gaactgtgct tcggcatggt gctggggctg acagcgtcaa cctggatcct gtggactgta
    43021 ctcggtagta acactctgct gttgatagat aaaaacatta tcaacattcc aaatctgatc
    43081 gaacagtacg gtgtggcgcg cgccattatc gaaacctggg ccgctctgcc actcagcacc
    43141 gccaccatgt ggggcttctt catcctctgc tttattgcca ccgttacgct ggttaacgcc
    43201 tgctcttata ccctggcgat gtccacttgc cgcgaagtac gcgatggtga agaaccaccg
    43261 ctgctggtgc gtatcggttg gtcaattctg gttggcatta tcggtattgt tttgctggcg
    43321 ctcggcggcc tgaaaccgat tcaaactgcc attatcgccg gaggatgccc gctgttcttc
    43381 gtcaacatta tggtgacgct ctcctttatt aaagacgcga aacagaactg gaaagattaa
    43441 ttaaccccca aaatatcaag aggttgaaag atggatttta atttaaatga tgagcaggaa
    43501 ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa actgggaggc ctattttacc
    43561 gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca aagcactggc ggatatgggt
    43621 atcgacagtc tgctgatccc tgaagagcac ggtggtctgg acgcggggtt tgttactctc
    43681 gccgccgtgt ggatggagct gggacgtctg ggggcaccaa cctatgtact gtaccagttg
    43741 ccgggcgggt tcaacacctt cctgcgcgaa ggcacacaag agcagatcga caagattatg
    43801 gctttccgcg gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc
    43861 tccgacgtgg gtagcctgaa aacgacttat acccgtagaa atggtaagat ttatcttaat
    43921 ggtagtaagt gttttattac cagcagcgcc tacaccccgt acatcgtggt gatggcgcgc
    43981 gacggggctt ctccggacaa acctgtctac accgaatggt ttgttgatat gagcaaaccg
    44041 ggcatcaaag tgaccaaact tgaaaagctc ggtctgcgta tggatagctg ctgtgaaatc
    44101 acctttgacg acgtggaact ggacgagaaa gacatgttcg gtcgggaagg taacggcttt
    44161 aaccgcgtca aagaagagtt cgaccatgaa cgtttcctgg tagccctgac caactacggt
    44221 acggcgatgt gcgcctttga agatgcggcg cgctacgcca accagcgcgt gcagtttggc
    44281 gaggctattg gtcgtttcca gttgattcag gaaaaattcg cccacatggc gatcaaatta
    44341 aactccatga aaaacatgct gtatgaagca gcgtggaaag cagacaacgg caccatcacc
    44401 tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga agtggtggat
    44461 agcgcaatgc aggtgctggg cggtgtcggg attgcgggca accaccgcat cagccgcttc
    44521 tggcgtgacc tgcgtgtaga ccgcgtctcc gggggatctg acgaaatgca gatcctgacg
    44581 ctgggtcgtg cggtgctgaa gcaataccgc taagtcaatg cgctgcctga tgcgacgctg
    44641 atgcgtctta tcaggcctac gggagcacga atgtaggccg gataaagcgt ttacgccgca
    44701 tccggcagtc atgcgctaca taaatttttc aggagcaaat aatggatcat ctacccatgc
    44761 cgaaattcgg gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa atcgccggac
    44821 cgtttgccgg gcaaatgttc gcagaatggg gcgcggaagt tatctggatc gagaacgtcg
    44881 cctgggccga caccattcgc gttcaaccga actacccgca actctcccgc cgcaatttgc
    44941 acgcgctgtc gttaaatatt ttcaaagatg aaggccgcga agcgtttctg aaattaatgg
    45001 aaaccaccga tatcttcatc gaagccagta aaggtccggc ctttgcccgt cgtggcatta
    45061 ccgatgaagt actgtggcag cacaacccga aactggttat cgctcacctg tccggttttg
    45121 gtcagtacgg caccgaggag tacaccaatc ttccggccta taacactatc gcccaggcct
    45181 ttagtggtta cctgattcag aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata
    45241 ccgccgatta cttttctggc ctgaccgcca ccacggcggc gctggcagca ctgcataaag
    45301 tgcgtgaaac cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg atgctgcgta
    45361 tgggccagta cttcatgatg gattacttca acggcggcga aatgtgcccg cgcatgagca
    45421 aaggtaaaga tccctactac gccggttgcg gtctgtataa atgcgccgac ggctacatcg
    45481 tgatggaact ggtgggcatt acccaaattg aagagtgctt taaagatatt ggcctcgcac
    45541 atctactcgg tacgccggaa atcccggaag gcactcagct tatccaccgt atcgaatgcc
    45601 cttacggccc actggtagaa gaaaaactcg atgcctggct ggcggcacat accatcgcag
    45661 aagttaaaga acgctttgcc gaactgaata tcgcctgtgc caaagtactg accgtaccgg
    45721 aactggaaag caatccacag tatgtggccc gtgaatcaat cactcagtgg caaacgatgg
    45781 atggtcgcac ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac cccggacaaa
    45841 tctggcgcgg aatgccctca catggcatgg acacggctgc cattttgaaa aatatcggct
    45901 acagcgaaaa cgacattcag gagttggtca gcaaaggtct ggccaaagtt gaggactaaa
    45961 cgcgtggcgt cagtcggctt gcctgactag cgcccgccca ataacaaacg aacaaaaatg
    46021 gatagaggtg caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctt
    46081 gcggacgttt acggtcataa aacggcgctg atttgtgaat ccagcggcgg agtcgttaac
    46141 cggtatagtt atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatacg
    46201 ctggggattc gcaaaggcga caaggttgca ctacatctcg acaactgccc ggaatttatc
    46261 ttttgctggt tcgggctggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg
    46321 ttacgcgagg aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt
    46381 gcgcaattct atcctatgta tcaacagatt cagcaggaag atgccactca attgcggcac
    46441 atttgcctga cagatgtggc acttcccgct gatgatggcg tgagttcgtt tactcaactg
    46501 aaaaatcaac aacctgccac cttgtgctat gcaccgccgc tatcgactga cgatacggcg
    46561 gaaattctct tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac
    46621 aacctgcgct tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc
    46681 tacctgacgg taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg
    46741 ttttctgccg gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga
    46801 caggtgcaga agtaccgcgc caccattacc gaatgtattc cgatgatgat ccgtacgttg
    46861 atggtgcagc cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat
    46921 ctcaacttgt cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt tcgcttgctg
    46981 acgtcttatg ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa
    47041 cgacgctggc cgtcgattgg tcgggcgggg ttttgctacg aagcggagat ccgcgacgat
    47101 cacaatcgcc cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg
    47161 aaaaccatct tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc
    47221 gacggctggc tacataccgg cgataccgga tactgcgacg aagagggctt tttttatttc
    47281 gtcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg
    47341 gaaaatatca tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat
    47401 tcgattcgcg atgaagccat caaagcattt gtggtggtga atgaaggtga aacattgagc
    47461 gaagaggaat ttttccgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat
    47521 ctggagatca gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg
    47581 aaataaatga aacagcaggg aactaccctg ccagctaata atcacgctat aaaacaatat
    47641 gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt
    47701 gaatcattac atctgacccg caatggatca attctggaaa ttacccttga tcgtccaaaa
    47761 gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt
    47821 gacgatccgc aattacgtgt cgccattatt accggtgccg gagagaagtt cttttccgcg
    47881 ggctgggatt taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt
    47941 ggatttgcgg gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac
    48001 ggctatgcct ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc
    48061 gataacgcca gcttcgccct gccggaagcc aagctgggca tcgttcctga cagcggcggc
    48121 gtgctgcgtc taccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc
    48181 agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag
    48241 gcggaactga tggataacgc ccgcgaactg gcgcagcagc tggttaacag cgccccgctg
    48301 gcgattgcgg cgctgaaaga gatcttccgc accaccagcg aaatgccggt agaagaagcg
    48361 tatcgctata ttcgcagcgg cgtgttgaaa cactatccgt cggttctgca ttcggaggat
    48421 gccattgaag ggccgctggc gtttgacgag aagcgcgatc cggtgtggaa aggacgttaa
    48481 caaccgtgag ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg
    48541 tccatcccag tgccgtcttg attggcgatg tgattgtggg agccggtgtc tacatcggcc
    48601 cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc
    48661 aggatggctg cattatgcat ggctactgcg acactgacac catcgttggg gaaaacggcc
    48721 atatcgggca cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga
    48781 tgaacagcgt gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgccatga
    48841 gctttgtcaa agcaggcttt cacggcgaga aacgccagtt gttgatgggt acgccagccc
    48901 gcgctgtacg cagtgttagt gacgacgagt tacactggaa acggttgaat accaaagagt
    48961 atcaggatct tgtcgggcgc tgtcatgcat cgttacatga aacacagcca ctgaggcaaa
    49021 tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt
    49081 gccggatgcg acctggttgc tggataagat gctacaacat cgcatctggc aagcaggacg
    49141 cccggtatca acgagataaa attaacgacg catactcttt gacaacatca tctgccactt
    49201 ctgctcgcgg ttaagttccg tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc
    49261 gactaattta tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac
    49321 cacttttacc agtcgctggc actgacatcc ccgtccttcc agcttattag ggatcatctt
    49381 aacttcgcag cttatttctg cgacttccga cagaatataa gtcagggtat taaccgcctt
    49441 gctgtgttca atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc
    49501 catcatccat tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg
    49561 aactccagtt tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg
    49621 ccatattaaa attgtggata tatcgatagt atgcaacttc accgtcgata agtgtgagct
    49681 aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta atgggcaaaa
    49741 aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg atgaaaaata
    49801 tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga aattactttt
    49861 tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca
    49921 taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc aatcgcgcga
    49981 cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg gtcctgaatg
    50041 tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc ttcgcccagc
    50101 acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag ttttgccgcc
    50161 aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg accgtgtttc
    50221 ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa ggtgcggccc
    50281 acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc aacacccggg
    50341 aatttattga acggcagcac cacttctttc accgagtagt acggcgggat aacttctttg
    50401 gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac ttttgccagc
    50461 ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg gttaacttca
    50521 atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc gcgcacctgc
    50581 aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc ctgacttaag
    50641 gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg ctcgatgtgc
    50701 tccatgatgc cgccaataag caccatttcg ccgtcgcaga tggcatccac gtcaacttct
    50761 accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac gctgaccgcc
    50821 gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc ccgaccgccg
    50881 agaacgtaag acggacgtac caccagcggg tagccaatct ctttcgcctt ctctaccgcc
    50941 atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg ctcaaccgca
    51001 tgctgaaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt gccgataacc
    51061 ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc gccgtactga
    51121 acgataacgc ctttcggctt ctcaatacgc acgatttcta gcacatcttc cagagttacc
    51181 ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc cgggttacag
    51241 ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg tacacagcag
    51301 tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac catgattttt
    51361 tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta catgtaagcg
    51421 gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg gtgcaggtca
    51481 tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt tgccagacgc
    51541 gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag gccagtgatg
    51601 cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac caggaaccaa
    51661 cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg gaaggcatcg
    51721 gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat tttggttaac
    51781 gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac ttccaggccg
    51841 cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc
    51901 gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa gttgaagcga
    51961 ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt acgtccgcca
    52021 gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc cgccacttta
    52081 gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac gcgtgggttc
    52141 atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac attggaacca
    52201 ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat gatttgatat
    52261 tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt atggatgccc
    52321 atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt atcacgcacc
    52381 acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag ctctttggtt
    52441 ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt ataagcgata
    52501 ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca
    52561 gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc ggtttccaga
    52621 ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg
    52681 gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg ctccagctcc
    52741 agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc cgggcgctct
    52801 ttttcaataa tcttgcgtac cacttcccag tgaatcggct cgatgtaggt cgcatcggcc
    52861 atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac ccggtaaccc
    52921 tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg
    52981 ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg ttttggcatg
    53041 gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac tcgataaagt
    53101 ggtcgaacaa cggcgcggcg tcgtgtggac cagggctggc ttcagggtgc ccctggaagc
    53161 tgaacgccgg tttatcggtg cgatgaatgc cctgtaacgt accgtcgaac agggatttat
    53221 gcgtgacacg caggtttgct ggtaatgttg cttcgtccac cgcaaaaccg tggttctggg
    53281 cggtgatcat caccacgttt ttctcaacat ctttaaccgg atggttgccg ccgtggtgac
    53341 caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga tgaccgaggc
    53401 agatgccgaa gaccggaata tcggtttcga ggaatttctg gatggcggta atggcgtaat
    53461 cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga ttcattttca
    53521 gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct ctgtccacca
    53581 acatgcgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg aacggcagct
    53641 cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc
    53701 tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga cctgggaacg
    53761 cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata atgcagccat
    53821 tctgtgcgcc tttctcgcgc agtaaacgtg tcagcttacg ggtatcgata tcggcaatcg
    53881 ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg aagttgctgg
    53941 caatcagcgg caggtcgcga atcaccagac cttgtgcatg tacctgagaa gattcttcat
    54001 cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg atttgacgag
    54061 aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg aaaacgactt
    54121 ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca
    54181 gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc actttatttg
    54241 catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac gaatttctgg
    54301 caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg ggatttttta
    54361 tattattttg tggctcatgc atgttttaca ggcattaagc ggcaaaaaga tcatataacc
    54421 tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaagcga gtagggaaaa
    54481 gtggaccaaa tggtcaaaat ttacattaaa acaacaaaaa ccgaagacaa aacatg
//
LOCUS       DAZFRN010000010        54460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFRN010000010 DAZFRN010000000
VERSION     DAZFRN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.010"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            3..395
                     /locus_tag="ACHKUB_000586"
     CDS             3..395
                     /locus_tag="ACHKUB_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO6653656.1"
                     /translation="MYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQNEIKNQHPLPE
                     GSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINEYPYRNT
                     HIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(449..1399)
                     /gene="corA"
                     /locus_tag="ACHKUB_000587"
     CDS             complement(449..1399)
                     /gene="corA"
                     /locus_tag="ACHKUB_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO6653657.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1551..1607)
                     /gene="ysgD"
                     /locus_tag="ACHKUB_000588"
     CDS             complement(1551..1607)
                     /gene="ysgD"
                     /locus_tag="ACHKUB_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO6653658.1"
                     /translation="MDTPSRYWLTILSFRINS"
     gene            1769..2533
                     /gene="yigE"
                     /locus_tag="ACHKUB_000589"
     CDS             1769..2533
                     /gene="yigE"
                     /locus_tag="ACHKUB_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO6653659.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(2680..4842)
                     /gene="uvrD"
                     /locus_tag="ACHKUB_000590"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2680..4842)
                     /gene="uvrD"
                     /locus_tag="ACHKUB_000590"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO6653660.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(4926..5642)
                     /gene="yigB"
                     /locus_tag="ACHKUB_000591"
     CDS             complement(4926..5642)
                     /gene="yigB"
                     /locus_tag="ACHKUB_000591"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO6653661.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(5642..6538)
                     /gene="xerC"
                     /locus_tag="ACHKUB_000592"
     CDS             complement(5642..6538)
                     /gene="xerC"
                     /locus_tag="ACHKUB_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO6653662.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6535..7242)
                     /gene="yigA"
                     /locus_tag="ACHKUB_000593"
     CDS             complement(6535..7242)
                     /gene="yigA"
                     /locus_tag="ACHKUB_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO6653663.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7239..8063)
                     /gene="dapF"
                     /locus_tag="ACHKUB_000594"
     CDS             complement(7239..8063)
                     /gene="dapF"
                     /locus_tag="ACHKUB_000594"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO6653664.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8100..8303)
                     /gene="yifL"
                     /locus_tag="ACHKUB_000595"
     CDS             complement(8100..8303)
                     /gene="yifL"
                     /locus_tag="ACHKUB_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6653665.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8462..8604
                     /locus_tag="ACHKUB_000596"
                     /pseudo
     CDS             8462..8604
                     /locus_tag="ACHKUB_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8766..9086
                     /gene="cyaY"
                     /locus_tag="ACHKUB_000597"
     CDS             8766..9086
                     /gene="cyaY"
                     /locus_tag="ACHKUB_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO6653666.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(9126..11672)
                     /gene="cyaA"
                     /locus_tag="ACHKUB_000598"
     CDS             complement(9126..11672)
                     /gene="cyaA"
                     /locus_tag="ACHKUB_000598"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO6653667.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            12059..13000
                     /gene="hemC"
                     /locus_tag="ACHKUB_000599"
     CDS             12059..13000
                     /gene="hemC"
                     /locus_tag="ACHKUB_000599"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO6653668.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            12997..13737
                     /gene="hemD"
                     /locus_tag="ACHKUB_000600"
     CDS             12997..13737
                     /gene="hemD"
                     /locus_tag="ACHKUB_000600"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO6653669.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            13759..14976
                     /gene="hemX"
                     /locus_tag="ACHKUB_000601"
     CDS             13759..14976
                     /gene="hemX"
                     /locus_tag="ACHKUB_000601"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO6653670.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            14979..16175
                     /gene="hemY"
                     /locus_tag="ACHKUB_000602"
     CDS             14979..16175
                     /gene="hemY"
                     /locus_tag="ACHKUB_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO6653671.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            16854..16991
                     /locus_tag="ACHKUB_000603"
                     /pseudo
     CDS             16854..16991
                     /locus_tag="ACHKUB_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(17099..18334)
                     /gene="aslB"
                     /locus_tag="ACHKUB_000604"
     CDS             complement(17099..18334)
                     /gene="aslB"
                     /locus_tag="ACHKUB_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6653672.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKVCWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(18481..18557)
                     /locus_tag="ACHKUB_000605"
     tRNA            complement(18481..18557)
                     /locus_tag="ACHKUB_000605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18521..18523),aa:Pro,seq:tgg)
     gene            complement(18600..18686)
                     /locus_tag="ACHKUB_000606"
     tRNA            complement(18600..18686)
                     /locus_tag="ACHKUB_000606"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18650..18652),aa:Leu,seq:cag)
     gene            complement(18707..18782)
                     /locus_tag="ACHKUB_000607"
     tRNA            complement(18707..18782)
                     /locus_tag="ACHKUB_000607"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18747..18749),aa:His,seq:gtg)
     gene            complement(18841..18917)
                     /locus_tag="ACHKUB_000608"
     tRNA            complement(18841..18917)
                     /locus_tag="ACHKUB_000608"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18881..18883),aa:Arg,seq:ccg)
     gene            complement(19020..20405)
                     /gene="yifK"
                     /locus_tag="ACHKUB_000609"
     CDS             complement(19020..20405)
                     /gene="yifK"
                     /locus_tag="ACHKUB_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6653673.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(20596..21336)
                     /gene="wecG"
                     /locus_tag="ACHKUB_000610"
     CDS             complement(20596..21336)
                     /gene="wecG"
                     /locus_tag="ACHKUB_000610"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO6653674.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(21339..22691)
                     /gene="wzyE"
                     /locus_tag="ACHKUB_000611"
     CDS             complement(21339..22691)
                     /gene="wzyE"
                     /locus_tag="ACHKUB_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO6653675.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(22688..23767)
                     /gene="rffT"
                     /locus_tag="ACHKUB_000612"
                     /gene_synonym="wecF"
     CDS             complement(22688..23767)
                     /gene="rffT"
                     /locus_tag="ACHKUB_000612"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO6653676.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(23764..25014)
                     /gene="wzxE"
                     /locus_tag="ACHKUB_000613"
     CDS             complement(23764..25014)
                     /gene="wzxE"
                     /locus_tag="ACHKUB_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO6653677.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAVASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(25016..26146)
                     /gene="rffA"
                     /locus_tag="ACHKUB_000614"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(25016..26146)
                     /gene="rffA"
                     /locus_tag="ACHKUB_000614"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO6653678.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(26151..26825)
                     /gene="rffC"
                     /locus_tag="ACHKUB_000615"
                     /gene_synonym="wecD"
     CDS             complement(26151..26825)
                     /gene="rffC"
                     /locus_tag="ACHKUB_000615"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO6653679.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(26803..27684)
                     /gene="rfbA"
                     /locus_tag="ACHKUB_000616"
     CDS             complement(26803..27684)
                     /gene="rfbA"
                     /locus_tag="ACHKUB_000616"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6653680.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(27703..28770)
                     /gene="rffG"
                     /locus_tag="ACHKUB_000617"
     CDS             complement(27703..28770)
                     /gene="rffG"
                     /locus_tag="ACHKUB_000617"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6653681.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(28770..30032)
                     /gene="wecC"
                     /locus_tag="ACHKUB_000618"
     CDS             complement(28770..30032)
                     /gene="wecC"
                     /locus_tag="ACHKUB_000618"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO6653682.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(30029..31159)
                     /gene="wecB"
                     /locus_tag="ACHKUB_000619"
     CDS             complement(30029..31159)
                     /gene="wecB"
                     /locus_tag="ACHKUB_000619"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO6653683.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(31215..32261)
                     /gene="wzzE"
                     /locus_tag="ACHKUB_000620"
     CDS             complement(31215..32261)
                     /gene="wzzE"
                     /locus_tag="ACHKUB_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO6653684.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(32273..33376)
                     /gene="wecA"
                     /locus_tag="ACHKUB_000621"
     CDS             complement(32273..33376)
                     /gene="wecA"
                     /locus_tag="ACHKUB_000621"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO6653685.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(33616..34875)
                     /gene="rho"
                     /locus_tag="ACHKUB_000622"
     CDS             complement(33616..34875)
                     /gene="rho"
                     /locus_tag="ACHKUB_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO6653686.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(34960..35061)
                     /gene="rhoL"
                     /locus_tag="ACHKUB_000623"
     CDS             complement(34960..35061)
                     /gene="rhoL"
                     /locus_tag="ACHKUB_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO6653687.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(35202..35531)
                     /gene="trxA"
                     /locus_tag="ACHKUB_000624"
     CDS             complement(35202..35531)
                     /gene="trxA"
                     /locus_tag="ACHKUB_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO6653688.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            35662..36927
                     /gene="rhlB"
                     /locus_tag="ACHKUB_000625"
     CDS             35662..36927
                     /gene="rhlB"
                     /locus_tag="ACHKUB_000625"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO6653689.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            37061..38545
                     /gene="gppA"
                     /locus_tag="ACHKUB_000626"
     CDS             37061..38545
                     /gene="gppA"
                     /locus_tag="ACHKUB_000626"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO6653690.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(38592..40613)
                     /gene="rep"
                     /locus_tag="ACHKUB_000627"
     CDS             complement(38592..40613)
                     /gene="rep"
                     /locus_tag="ACHKUB_000627"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO6653691.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            40830..41279
                     /locus_tag="ACHKUB_000628"
     CDS             40830..41279
                     /locus_tag="ACHKUB_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO6653692.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVNNGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            41478..41759
                     /gene="ppiC"
                     /locus_tag="ACHKUB_000629"
     CDS             41478..41759
                     /gene="ppiC"
                     /locus_tag="ACHKUB_000629"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO6653693.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(41846..43321)
                     /gene="ilvC"
                     /locus_tag="ACHKUB_000630"
     CDS             complement(41846..43321)
                     /gene="ilvC"
                     /locus_tag="ACHKUB_000630"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO6653694.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQDKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            43471..44364
                     /gene="ilvY"
                     /locus_tag="ACHKUB_000631"
     CDS             43471..44364
                     /gene="ilvY"
                     /locus_tag="ACHKUB_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO6653695.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(44416..45960)
                     /gene="ilvA"
                     /locus_tag="ACHKUB_000632"
     CDS             complement(44416..45960)
                     /gene="ilvA"
                     /locus_tag="ACHKUB_000632"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO6653696.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(45963..47813)
                     /gene="ilvD"
                     /locus_tag="ACHKUB_000633"
     CDS             complement(45963..47813)
                     /gene="ilvD"
                     /locus_tag="ACHKUB_000633"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO6653697.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(47878..48807)
                     /gene="ilvE"
                     /locus_tag="ACHKUB_000634"
     CDS             complement(47878..48807)
                     /gene="ilvE"
                     /locus_tag="ACHKUB_000634"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO6653698.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(48827..49090)
                     /gene="ilvM"
                     /locus_tag="ACHKUB_000635"
     CDS             complement(48827..49090)
                     /gene="ilvM"
                     /locus_tag="ACHKUB_000635"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO6653699.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(49087..50733)
                     /gene="ilvG"
                     /locus_tag="ACHKUB_000636"
     CDS             complement(49087..50733)
                     /gene="ilvG"
                     /locus_tag="ACHKUB_000636"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO6653700.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(50736..50786)
                     /gene="ilvX"
                     /locus_tag="ACHKUB_000637"
     CDS             complement(50736..50786)
                     /gene="ilvX"
                     /locus_tag="ACHKUB_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO6653701.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(50873..50971)
                     /gene="ilvL"
                     /locus_tag="ACHKUB_000638"
     CDS             complement(50873..50971)
                     /gene="ilvL"
                     /locus_tag="ACHKUB_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO6653702.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            51324..52844
                     /gene="yifB"
                     /locus_tag="ACHKUB_000639"
     CDS             51324..52844
                     /gene="yifB"
                     /locus_tag="ACHKUB_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO6653703.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(52869..53207)
                     /gene="maoP"
                     /locus_tag="ACHKUB_000640"
     CDS             complement(52869..53207)
                     /gene="maoP"
                     /locus_tag="ACHKUB_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO6653704.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            53326..54165
                     /gene="hdfR"
                     /locus_tag="ACHKUB_000641"
     CDS             53326..54165
                     /gene="hdfR"
                     /locus_tag="ACHKUB_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO6653705.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(54261..54336)
                     /locus_tag="ACHKUB_000642"
     tRNA            complement(54261..54336)
                     /locus_tag="ACHKUB_000642"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54301..54303),aa:Trp,seq:cca)
     gene            complement(54345..54421)
                     /locus_tag="ACHKUB_000643"
     tRNA            complement(54345..54421)
                     /locus_tag="ACHKUB_000643"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54385..54387),aa:Asp,seq:gtc)
BASE COUNT        13321 a        14752 c        13826 g        12561 t
ORIGIN      
        1 ttttgtatac atactctgat gctggcaatt cagtctttct ggtcgttgat catctgccat
       61 ggactgatag cgataaaata aattggtatc tgaagcatca gaatgaaatt aaaaatcaac
      121 atcccctacc agaaggtagt tggcatacct ggtatgtcat tgatatcggt aatggcttca
      181 ctgactataa aaaatatatc gaagggccgt atgaagatct ctactgtttc ccgacaatta
      241 agagcaatga taattgcatc gtgaaaaatt acttaatggt tattaatgaa tacccttatc
      301 gtaacactca tattggtatt aacgatttta ctgagtatca attaacacaa gaaaataaaa
      361 tagagcgagt tttcaatccg catgacttta aataagacaa tttttagaaa taagccggat
      421 aaaaaaatag ctttatccgg cactcatatt acagccagtt cttgcgctta aagtacagat
      481 acggtgccag gcccgcgagg atcataaaga taatcgcgcc tgggtagccg aaactccact
      541 tcagttctgg cataaactca aagttcatcc catagctgga agcaacgagc gtcggcggca
      601 ggaataccac ggataccacc gagaagattt tgatgatgcg gttctgctcg atgttgataa
      661 agcccatcgc cgcctgcatc aggaagttca ccttctggaa cagggattcg ttatgcggca
      721 gcagggattc gatatctcgc aggatttcac gcgcctgctc cagttgccca cccggtaaac
      781 gcgccttgcg taccaggaag ttgagcgcac gctgggtatc catcagacac aggcgaactt
      841 tccagccgat atcttccagt tccgccagcg tggagagcgc ctcatcgtac tcatcgccct
      901 gatgcccttc cataattacc cggctcaact gctccagatc gctgtagata ttttcaattt
      961 catctgccag ctgttcgatt ttggtttcga acagatccaa cagcagctcg taggcgttac
     1021 cgtccaccat tgactggcta cgggcgcgca tacgatacag acggaatgca ggcagttcac
     1081 gctcacgcag agtaaacaga cgaccatcac ggatggtaaa tgccacagtg gagttaccgg
     1141 cgtgatcttc cgcatcttca aagaagaaga aggagtgaat atgcaggccg tcatcgtctt
     1201 caaagaaacg tgccgatgct tcgatgtctt ccagttccgg gcgggttgcc aggctctggc
     1261 caagttcaga ttgtacgcgc agtcgctcgt cgtcgtccgg ttcgacaaga tcaatccata
     1321 ctgcatttac aaggggttgt gactcttcga cttccagccg ggtcagtcgg ttattttcca
     1381 gttgaaatgc gctcagcatg accgggactc ccaatgctta aaatatcgga cagttcggtg
     1441 ggcacacaga aacaaattgg gtttcagacc attaaacagc ctgactcagc gcgacgggaa
     1501 aaattgaggt cgctgacaac cgctaaggct atcagcaaaa agggatagcc ttaggagttg
     1561 atcctgaatg acaggatagt gagccagtat ctactgggtg tgtccaaggc gaatgtcctc
     1621 ttagagtgat cgtgcgcgca tgttacgcca gcaaaatttt gccgtcaaca cgcaacagaa
     1681 caccacagaa caatagttga acttcgcgct ttttggtact cgtcaaggtc gctaacagga
     1741 taaaaaaatc caacatccag gacctataat ggcgcatcgg ctactcattg gtaaaggaat
     1801 gatcaccttg aatctcaaac ggatttttct caccctcacc ttacttccct tgtttgctgt
     1861 tgccgctgat gattgcgcac tctcagatcc gacgctgacc gtacaggcgt ataccgttaa
     1921 tcctcaaaca gagcgggtga aaatgtactg gcaaaaagcc aatggcgaag cgtggggaac
     1981 tttacatgct ctgctggcgg atatgaatag tcagggtcag gtgcagatgg cgatgaacgg
     2041 cggcatctat gatgaaagct atgcgccgct cggtttgtac atcgaaaacg gtcagcagaa
     2101 ggtggcgtta aatctcgctt caggtgaagg gaatttcttt atccgtcctg gcggcgtgtt
     2161 ttatgtcgcg ggagataaag tcggcatcgt tcgtctggat gccttcaaaa ccagtaaaga
     2221 gattcagttt gcggtgcagt cagggccaat gttgatggaa aacagtgtaa ttaatccgcg
     2281 tattcatccc aacgtcgcct cacgtaaaat tcgtaacggt gttgggatta ataaacatgg
     2341 gaatgccgtg tttttgttga gccagcaggc gacaaatttt tatgattttg cctgttatgc
     2401 caaagcgaaa ctgaacgttg agcagctgct ttatcttgat ggcacgattt cacatatgta
     2461 tatgaaaggc ggggcaatcc cgtggcaacg ctatcctttt gtcaccatga tttccgtaga
     2521 gcggaaaggt taaaacgata gttttgtgtc agatactgaa gatggcgcag atgccggatg
     2581 cggcgtaaac gccctatccg gcctacatga agttgcaatt tattgaatat gaatgatttt
     2641 ttaggccaaa taaggtgcgc agcaccgcat ccggcaacgt tacaccgact ccagccgggc
     2701 gtatgccgcc accagccatt taataccctg gccctggaat gccacctgca aacggctatg
     2761 ctcgccgctg ccttccatat tgacgatggt gccttcacca aacttagcgt ggcgtacacg
     2821 ctgaccgagc ttgtagccgc tgtcgttctc gaccatcggc gtccccatcc gttgatggct
     2881 gaccggacga cttaccgtag cgcgcaggcg cacctcttcc acacactctt ccggcagctc
     2941 gccgataaag cgcgacgggc gatggtaaac ctctttacca tacagacggc gggtttccgc
     3001 gtaggtcagc gtcagtttct gcatcgcgcg ggttacacca acgtaggcca gacggcgttc
     3061 ttcttccaga cgcccgcctt catccagcga catctggctt gggaacatac cctcttccat
     3121 accgacaata aacacctgcg ggaactccag gcctttcgcc gagtgcagcg tcatcaactg
     3181 cactgcatcc tgccaggtat ccgcctgccc ctcgcccgct tccagcgccg catgggagag
     3241 gaatgcctgc agcggcatta aatcttcatc ttcttcgttg tagctgaact ggcgcgttgc
     3301 cgtcaccagt tcctctaagt tttcgatacg cgtctggcct ttttcgccct tctcctgctc
     3361 gtacatggtg cgcaggccgg agtctttaat tacccggtca gtctgtacat gcagcggcat
     3421 atcggcagtt tcttgcgcta aggcatcgat taattccata aatcgctgca aggcgctggc
     3481 agctcgcccg gcgagggctt tttcctgcaa cagttcacga catgcctgcc agagtgttaa
     3541 ctggcgatcg cgcgatgtct gacgtaccac gtccagcgtc cggtcaccaa taccccgcgt
     3601 tggcgtattc accacgcgct caaacgccgc gtcgtcgtta cggttggcaa tcagacgcag
     3661 atacgagagc gcgtctttga tttcctggcg ttcgaagaag cgcatcccgc cgtaaatacg
     3721 gtacggcata ctggcctgca acagtgcttc ttccagtaca cgcgactggg cgttactgcg
     3781 gtagagaatg gcgcactcgg caagcgcccc gccgttgtcc tgccaggttt tgatgcggtt
     3841 aaccacaaaa cgcgcttcat cgagttcgtt aaaagcgcaa tagagggaaa taggctcacc
     3901 gtccgcgcca tcggtccaca gttttttacc cagacgcccg ttattgtttt caatcagggc
     3961 gttagcggcg ctcagaatat tgctggtaga gcggtagttt tgctccagac gaatagtttc
     4021 ggcaccgggg aaatcattaa ggaaacgctg aatattctcc acctgcgccc cgcgccagcc
     4081 gtagattgac tggtcgtcat caccgacgat catcactttg ccggtgtcgc ccgccagcag
     4141 gcggatccac gcgtactgaa tgttgttggt atcctggaat tcgtccacca ggatattggt
     4201 aaaacgttcg cggtagtgtt gtaggatatg cggcttgtta agccacagtt cgtgagcgcg
     4261 cagcagcagc tcggcgaagt ccaccaggcc cgcgcggtca cacgcttcct gatacgcctg
     4321 atacaccttc tgccaggtct gctccaccgg attaccgtag ctttgaatat gatgcggacg
     4381 caggccttca tctttctggc tgttgatgta ccacattgcc tggcgcggcg gccactgctt
     4441 ctcgtcgagg ttcatggctt tgatcagacg cttaagcagg cgcagctggt cctcgctatc
     4501 gaggatctgg aaatcctgcg gcagattggc gtccatatgg tgcgcacgca gcaggcggtg
     4561 cgccagcccg tggaaggtgc cgacccacat gccgccctgg ctggtgccca tcagttgccc
     4621 gatacgatga cgcatctccg ccgccgcttt gttggtaaac gtcaccgcca taatcgagta
     4681 tggcgagcag ttttccacgc tcatcagcca ggcgatacga tgcaccagta cgcgcgtctt
     4741 accactgccc gcgcccgcca gcaccagaag gttgctgcgt ggcgcggcca ccgcttcgcg
     4801 ctgtttgtca ttaaggctgt cgagcaggta agaaacgtcc attggcaccg ccgcgtaaaa
     4861 ataggttggg cggagaagcg caacgccctc cgccaaaaag ctgggtatat atacagattt
     4921 gctgattata tcagcgaggt cagagatgcc aaccgggaaa tttccagatg cggcagtaaa
     4981 cggctgtccc aggtttgcat cagatcgcca ttttccggtc tgatccaaca agcctgcatt
     5041 ccgctgcgaa ttgccccacc cacgtcagtg gtgagatcgt ccccaacatg taagatctcg
     5101 ccgatcggca cgttgagttt ttccgcagcc aaaaagtaca tatcgctgaa cggttttgag
     5161 cgcccgtgcg ggccagcgcg cagcacaaac tcaaaataat cccccaaacc aaacagttcc
     5221 ggctgggcgt taccgttggt gatcgccacc agcggccatt tcttcgccag ctgttttaag
     5281 gtgtcgtgag tttgctgcgg gacgtcgatt cggctgcgcc atttggcaaa gttaatcatt
     5341 gctgcgtgtg cgcctgcact ggcttcttcg gcactcagcc cggcgtcgag catcgcttgt
     5401 tcaatcgaac gaaaacgcca acgcgtcacg tcgtgataaa tctcgggttc cgcttcccgt
     5461 accgcctggc gcaggcgttg cagatcttca ttctggaagc tgcgcagcgc cggatgataa
     5521 ttttgcacaa aggtaagcgc ctctcgctcg gtgcgcaaaa tcaccggacg gttatcgtaa
     5581 agggtatcat ccaggtcaaa ggtgagcgcc gagatgcgcc ccaaaggccg gtaaaaacgc
     5641 attatttccc ccgtttggcg cgtggatgcg ccgcatcgta caccgaggca aggtgttgaa
     5701 aatcaagatg agtatagatt tgcgtggtgg agaggttggc atgacccagc agctcctgca
     5761 caccacgaag atcgccgctc gactccagca tatgcgtagc gaacgagtga cgtaatttat
     5821 gtgggtgaac gtgattattc agcccttgtt ttatgcccca ttcggcaaag cgtttctgca
     5881 cattacgcgc ggagatacgc ttgcccagtt tcgacagaaa aagcgcgtcg tcttcgctac
     5941 caaacaggtc gcgcaaatca agccagtgct caatccacgc cacagcgttg cgaccaatcg
     6001 gcaggcggcg ctctttgctg cctttcccca taacccacac ttcaccagac tccaggtcga
     6061 ggtgtttaat atccagcccc accagctcag aaagacgcag acccgcgccg tacatcactt
     6121 ccagcattgc acggtcgcgt acagcgaggg gatcattgat atcaatgtcc agcagccgat
     6181 tcatatcgtc gacgtcgata tttttcggca gatgacgcgg cgctttcggt gccgaaacac
     6241 ctttcgccgg gttagctttg agttcgttct ggctgaccag ccagtcaaaa aagctacgta
     6301 gcgcagaaag ccgtaacgcg aggcttgctg ctcccagccc tttacggcga ctgcgtacag
     6361 caaaattgcg caccatcgtc acatcacatt gctgccagct ttgcaggccg ttttcgctgg
     6421 caaaattgat gatcgcctca agctgacgct ggtagttaag cagggttatc gggctaagct
     6481 ggcgctccac gctcagataa cgtaggtagc gttctacatc ggtgtgtaaa tcggtcatac
     6541 gcgttcaatc caacgctcca gaagctccgg caacatcagc gcaatttcat gaagtaactg
     6601 cgttccttgc ccttgttgat agtgactggc atcgcgactg gtaaacagca cgacacccaa
     6661 atcagcatcg cttcccagca tcgacatcgc caccgatccc accgctttcg cttccggtag
     6721 caccaccagc agctctggtc cgttaagcgg cccaagatag tgctgttcct gccccaaacg
     6781 ctgaatacgc agcggttcga aagactgacg gcttaatgcc agatgagtgt ggttcgacgg
     6841 cgcacctaag cgccagcgat ccggaaacag gcgcagactc gcacctgcca ggccgagatc
     6901 gcgcgcccag cggtgaaagc gcatcagcat atcgtcgaga ctgctggcgg cggtgagact
     6961 gcgctgcagg tagagtaggc gataaaacag gccttcgttg gcgatagcct gttccatcaa
     7021 cagcgccatg ttctcttcca gaacatgaat atgattacgt gcgcgggcca tgtgccactc
     7081 gaccaacgaa acggtgccgc gtaccggatg cggcacacgt atcgcttcta ctgcgcgcgc
     7141 attacggata aaaaactcag gatttttaat cagataatcg acaaccgccc ggtcatcaag
     7201 ctccgtgagt gtttcctgca gttcttcccc tggttgcttc atagatgaat aaatccgtcg
     7261 tagacatgta ccgccgggcc agtcatatat aacgggtgac ccggaccttt ccaggcgata
     7321 tcaagacgac cgccggggag ttccacgcgt acttcttcgg ccagcaaacc ttgctgaatc
     7381 cctactgcaa ccgccgcaca cgcgccgctg ccgcaggcct gggtttctcc tgccccacgc
     7441 tcataaacgc gtaaacgaat atgctcgcgc ttaaccactt gcataaaacc gatattggct
     7501 cgctccggaa aacgctcgtg gctttccaga acaggaccaa gcgtttctac cgccgcggta
     7561 tcgacatcat cgacctgaat cacgcaatgc ggatttccca tcgacaccac gccgcataag
     7621 attgtctgct cggcggcgcg cataatatag gtcttttccg ctttgttagc gcgaaacggc
     7681 acggcggaag gttcgaagtt gggttcgccc atatttacgc ggaccagatc atcatcggtg
     7741 acggtcagaa ccatccgccc gttggcggtg ctgacgcgga tatcgcgctt attggtcagc
     7801 cctttcagac gcacaaaacg ggcaaagcag cgcgcaccat tgccgcactg cgccacttca
     7861 ctgccatcag cattgaaaat gcgatagtga aaatccagtt caggatcata cggcggctca
     7921 accaccagca gttggtcaaa ccctactccc aggtgccgat cagccaggcg acgaatcagc
     7981 tccggtgaaa aaaagacatt ctgcgttacc gcgtcgacga ccataaaatc gttgccaagg
     8041 ccatgcattt tcgagaactg catcatttac tccaatcacg cgggtacaga aactgacttt
     8101 tagtaattca cctgggatgg accatcgcca gtggcgcgat cgtttttatc cggcaccgtg
     8161 gattgcgttt gcgtctctac cggtttggtc ggcggcggtg cgtttttatc tgcaggcggg
     8221 aaatagagcg gacctttcag accgcagccc gtcaggctga agagagtaag taatacagtg
     8281 agtgccttaa acacgttttt cattatggat tgcctgtaaa gttcatcgct ttctgctttc
     8341 tatcatcgca ggagaagacg taaaagcaag cgtttagcga ctctttatga agtctgaaaa
     8401 gtgaggaaat gctgcgagta taacgcttac tcgtggggat ttatgttttg ctggtagttt
     8461 cgtgatcggc acctcgcgcc aggatggctt tatgcactat cgcaatgtag tttgtttgtt
     8521 ggctttttta gttccgtcag cgtgaggatg ctcgctcggt aaacctgatc ataatgttta
     8581 ccagaaacag ggaaaggcgt ggtgtaactg cgccctaatg aagagacaaa tctttcgcta
     8641 cactgagaca tcattattcc ggtgacggat gaatcgtgcc ggaacgcagc cgcagaggtg
     8701 caaactttga agccctgacc ccatgccgct atactgccgc catcacttaa aaacaggata
     8761 caacaatgaa cgacagtgaa tttcatcgcc tggctgatca actctggctg accattgaag
     8821 aacgcctgga cgactgggat ggcgacagcg atatcgactg cgaaatcaac ggcggcgtac
     8881 tgaccattac ctttgaaaac ggcagcaaaa tcattattaa ccgccaggag ccgctgcacc
     8941 aggtgtggct ggcaaccaaa cagggcggct accattttga cctaaaaggc gatgaatgga
     9001 tttgcgatcg cagcggcgaa accttctggg atttgctgga acaggcggcg acacagcaag
     9061 cgggtgaaac agtcagtttc cgctaagatt gcatgccgga taagcctcgc tttccggcac
     9121 gttcatcacg aaaaatattg ctgtaatagc ggcgtatcgt gatcctgatt ggcaggcggc
     9181 atgttaccga tagattttgt gcggaacgga atcacctgtt cacgaccatc aaccttcaca
     9241 atctgataga actgcggcag gttgaagttg atgaagcttg agccgtaggt gaagcggtcg
     9301 tgcgatgacg agtagaagcg actgacgtca cgcaccagtt cctctttgct gccttcgcag
     9361 tggtgataca cctcaacccg gttgctttcg tcgagaatgt agatattaaa gccattctcg
     9421 tcttgcgttt cttcgaagaa gaactggatg atcccttcac tagcaaagcc gtccaccacc
     9481 gccggtaatt tgacgtgatt ggtttcaacc tgcactgaca agccgtgcag tttgttatgc
     9541 gaaatagcgc cataaaactc gatggcgttt tccagttttt gtaccgatac attcaggcgt
     9601 tcgaagaaca acccccaggt ttgaccagaa acgcgcagcg ccttgaaacg cccggtttcc
     9661 tggcgggtgc tggaaaggcg cagttcaata cactcagaaa ccagttgctg cacgcgagta
     9721 cggattaacc cgcgcagatg ctggctataa cagaagactt ccacgctatc tggcggtgcg
     9781 gcgtcctgat gcattttgcc gagaatagtt ttcagggctt cgatcatcga ttgctcgccg
     9841 ttgaagtgca gcgtacgcac ttcgttccac gagttgcggt acagcaggtc aacgctacct
     9901 accaggcaat tttgattctc gccaaagctg aagacatcca gcttacggaa atcaaaatgc
     9961 accacctgat tgcggaacgc cgctgtcggg tcatattcca ggttaacgat aatcgccaga
    10021 tggcggatct cacacgggct gtagagcgcc ttcggtgtcg gtgcaggtaa gcgcagcggg
    10081 aaatggtgcg acacgtcggc gaccatctcc tgcaacttag gcaaatcgac aatgccgtta
    10141 cctttaatat acaaacgggt gcgcgaggtc agcaggccgt taaaccatgc ccacgccacc
    10201 agtttattca ggtaacggtt atattccagc ggctgatggc tgatgatcga ctcaatattt
    10261 ggcgcgcggt tatacagata ccaacctgaa cggttagcac ggcccggcgg cacataaata
    10321 aaggtcagat tcggttccga gagatcgggt gaaatctgcg ggtttaccag cgtcacttta
    10381 cctggtaatg cttcaaacgc ggcatacagc ttacgcgtca gcacgccgat atcctgcgga
    10441 ctggcggaga cgctaaggtt attgcgacgc gcaaagcgga tcagattacg gtagctctgc
    10501 atcatcgcgt cgagcaactc gttgtgcgcc tcacgcacct gatcaatctt ccagttagcg
    10561 cggttatcga gcattgccag acgagcttcg tcccaacccc actcgctcac taactggctc
    10621 aacactgcgc gacgccagcc tacgcaggcg cgttcacggc tgagcttttc gcacactttt
    10681 aaatagaagc agcgacgtac taaatccaga cgggtaaaat cttcaatcgc cgtcaggtat
    10741 tcagtaacac gctccagcat catgcagtat ggatcgagac caaacgatac aatctcgccg
    10801 tcgtgcaaac gctgtttgat atctttcgcc agcagacgtg ggttcgggta ttcccaggaa
    10861 taggcttcca gcagcagtgt tttcagtacc gctttgtatg gggaatcgat actcttgtag
    10921 agctgccaaa ggctggcacc aaagtactct tcagcagaaa gcgagcttaa gccaccgaga
    10981 tccagccatt catttggcgt cagcacgccc tgcgcgtaaa gcgtcatcac atagtcgtcg
    11041 taatgctctt cttcgtcgca cggcaccata ttccacagaa tacgcttacc ggcgagacgc
    11101 acggcggtac gataaaattc gtcaagcagc agtatatgct gggtggagcc acaatcttcg
    11161 ccccccaggc tgccgctttc attatgacgg aagcggtttt catcaatcag gaagaagctg
    11221 acttccacac ccagcgaggc ggcccagctt tccagcagac tacatttacg ttgtagtaac
    11281 tggcgctctt cgctatcgag ccaggattga tgacagaccc agatatccag gtcagaggaa
    11341 caactttgcc ctacggacga ggtgctgccc atggtgtata caccagtaat tggaagctca
    11401 cctttcggtg ggtcctgtac tgacattcca cgatacagtt caagctcgtt caggtagtgg
    11461 cgttgagttt catcaggcgt gtaaaggcaa atgcctttgg gaacgttacc atcaaggtaa
    11521 cccggcatta gcggatggtg atagtgcaac aatgtcggca gtagactgta gacctgttgg
    11581 aatgcaggcc ccatagcagc aagcgcgcga tccacacgca attgatttat ggcatccagt
    11641 ctctgtttca gagtctcaat atagaggtac aagacgtatc gcctgatttg ctacccgtca
    11701 tgactgtgat tccgccaaca tcaacggtaa cacgcggcat tcgggatatt tcgtatgtca
    11761 aaggtaaccg ttaccacttt tcgcgcctgg tttttttagt ttcacgacga aaaaatggtc
    11821 taaaacgtga tcaatttaac accttgctga ttgaccgtaa agaaagatgc gctacataca
    11881 agtgtagcac cgtttattct ctgtaaattc cttattacga cggcgtgaaa cgcctgtcag
    11941 gatccactgc cagacctcat tttacggttt gcgcaggcgt ctacgtttca ccacaacact
    12001 gacatcactc tggcaaggat gttaggatgg accacggatg ataatgacgg taacaagcat
    12061 gttagacaat gttttaagaa ttgccacacg ccaaagccca cttgcactct ggcaggcaca
    12121 ctatgtcaaa gacaagttga tggcgagcca tccgggcctg gtggttgaac tggtaccgat
    12181 ggtgacgcgc ggcgatgtga ttcttgatac gccgctggcg aaagtaggcg gaaaaggctt
    12241 atttgtaaaa gagctggaag tcgcgctcct ggagaatcgc gccgatatcg ccgtacattc
    12301 aatgaaagat gtgccggttg aattcccgca aggtctgggg ttggtcacta tttgtgagcg
    12361 tgaagatcct cgcgatgcct ttgtgtccaa taattatgac agtctggatg cgttaccggc
    12421 aggcagtatc gtcgggacgt ccagtttacg tcgccagtgc caactggctg aacgccgccc
    12481 ggatctgatt atccgctccc tgcgcggcaa cgtcggcact cgcctgagca aactagataa
    12541 cggcgaatac gatgccatca ttcttgccgt agccggacta aaacgtttag gtctggagtc
    12601 ccgcattcgc gccgcgttac cacccgagat ttctcttccg gcggtaggac aaggtgcagt
    12661 cggtattgaa tgccgccttg atgatacgcg cactcgcgag ctgcttgccg cgctgaatca
    12721 ccacgaaact gcactgcgcg ttaccgcaga acgcgccatg aatacccgtc tcgaaggtgg
    12781 atgtcaggtg ccaattggta gctacgccga gcttattgat ggtgaaatct ggctgcgtgc
    12841 gctggtcggc gcgccggacg gttcgcagat tattcgcggt gaacgccgcg gtgcgccgca
    12901 aaatgccgaa caaatgggga tttcgctggc agaagagcta ctgaataacg gcgcgcgcga
    12961 gatcctcgct gaagtctata acggagacgc cccggcatga gtatcctggt cacccgcccg
    13021 tctcccgctg gagaagagtt agtgagccgt ctgcgcacac tggggcaggt ggcctggcat
    13081 tttccgctga ttgagttttc tccgggtcga caattaccgc aacttgctga tcaactggcg
    13141 gcgctggggg agagcgatct gttgtttgcc ctctcgcaac acgcggttgc ttttgcccaa
    13201 tcacagctgc atcagcaaga tcgtaaatgg ccccgactac ctgattattt cgccattgga
    13261 cgcaccaccg cactggcgct acataccgta agcggacaga agattctcta cccgcaggat
    13321 cgggaaatca gcgaagtctt gctacaatta cctgaattac aaaatattgc gggcaaacgt
    13381 gcgctgatat tacgtggcaa tggcggtcgt gagctaattg gggataccct gacagcgcgc
    13441 ggtgctgagg tcactttttg tgaatgttat caacgatgcg caatccatta cgatggtgca
    13501 gaagaagcga tgcgctggca atcccgcgag gtgacgatgg tcgttgttac cagcggtgaa
    13561 atgttgcagc aactctggtc gctgatccca caatggtatc gtgagcactg gttactacac
    13621 tgtcgactat tggtcgtcag tgagcgtttg gcgaaactcg cccgggaact gggctggcaa
    13681 gacattaagg tcgccgataa cgctgacaac gatgcgcttt tacgggcatt acaataactc
    13741 tcataacagg aagccataat gacggaacaa gaaaaaacct ccgccgtggt tgaagagacc
    13801 agggaggccg tggacaccac gtcacaacct gtcgcaacag aaaaaaagag taagaacaat
    13861 accgcattga ttctcagcgc ggtggctatc gctattgctc tggcggcggg cgtcggtttg
    13921 tatggctggg gtaaacaaca ggccgtcaat cagaccgcca ccagcgatgc cctggctaac
    13981 caactgacgg cattgcaaaa agcccaggag agccaaaaag ccgagctgga aggcattatt
    14041 aagcaacaag ctgcacagct taagcaggcg aatcgtcagc aagaaacgct ggcaaaacag
    14101 ttggatgaag tccaacaaaa ggtcgccacc atttccggca gcgatgctaa aacctggctg
    14161 ctggctcagg ccgattttct ggtgaaactc gccggacgga agctgtggag cgatcaggac
    14221 gtcacgaccg ctgcagcgtt gctgaaaagt gcagatgcca gcctggcgga tatgaatgac
    14281 ccgagtctga ttaccgttcg tcgggcaatt accgatgata tcgccagcct ttctgcagtt
    14341 tcgcaggtgg attatgacgg catcatcctt aagcttaatc agctttcaaa tcaggtagat
    14401 aacctgcgtc tggccgataa tgacagcgat ggttcgccga tggattccga cggtgaagag
    14461 ctttccagtt ccatcagcga atggcgtatc aatctgcaaa aaagctggca gaactttatg
    14521 gacaacttca ttacgattcg ccgtcgtgat gacaccgccg taccgctgtt agcgccaaat
    14581 caggatatct atctgcgcga aaatattcgc tctcgcctgc tggtcgcagc acaagctgta
    14641 ccgcgtcacc aggaagagac ttatcgccag gcgctggaga acgtctccac ctgggtacga
    14701 gcttactacg atactgatga tgccaccacc aaagcgttcc tcgacgaggt ggaccagtta
    14761 agccagcaaa atatctcgat ggatcttccg gaaaccctgc aaagccaggc gatgctggaa
    14821 aaactgatgc agacccgcgt gcgtaacctg ctggcacaac cggcagcggg gacaacggaa
    14881 gctaaacctg cacctgcacc tgcacctgca cctgcacctg cacctgcacc tgcaccgcaa
    14941 ggtgatactc cggcagccgc gccgcaagga gaataaccat gctaaaagtg ttattactct
    15001 ttgttttgct gattgcgggg atagtggttg gcccgatgat tgccggtcat cagggttacg
    15061 tgctgatcca gactgacaac tacaatatcg aaaccagcgt cacgggcctg gcgattattt
    15121 tgattctggc gatggtagtg ctgtttgcca ttgagtggct actgcggcgg atcttccgca
    15181 caggcgcaca cacccgtggg tggtttgtcg gacgtaagcg tcgccgtgcc cgtaagcaga
    15241 ccgaacaggc gctgctgaaa ctggcggaag gcgattatca gcaagttgaa aagctgatgg
    15301 cgaaaaatgc cgatcacgcg gaacaaccgg tggtgaacta tctactggct gccgaagccg
    15361 cgcaacaacg tggtgatgaa gcacgcgcca accaacatct ggaacgcgca gcagagctgg
    15421 ccggcaatga caccattccg gtagaaatca cccgtgtacg tctgcaactg gcccgtaatg
    15481 aaaaccatgc tgcacgccac ggcgtggata agctgctgga agttacgcca cgccatccgg
    15541 aagtattacg tctggcggaa caggcgtata tccgcacagg tgcatggagt tctctgctgg
    15601 atatcatccc atcaatggcg aaagcccatg ttggtgatga agaacatcgt gcaatgctgg
    15661 aacaacaggc atggattggc ctgatggatc aggcgcgtgc cgataacggt agtgaaggtt
    15721 tgcgtaactg gtggaaaaac caaagccgga aaacgcgtca tcaggtagcg ttgcaggtgg
    15781 caatggcgga acatcttatt gaatgtgacg atcatgatac tgcccagcaa attatcatcg
    15841 atggcctgaa acgccagtac gacgatcgcc tactgctgcc gattcctcga ctgaaaacca
    15901 acaatccgga acagctggaa aaagtgctgc gccagcagat caaaaacgtc ggcgatcgcc
    15961 cgctgttgtg gagcacactg ggccagtcac tgatgaagca cggagaatgg caggaagcat
    16021 cgctcgcctt ccgcgcggcg ctgaaacaac gtccggacgc ctacgattac gcatggcttg
    16081 ccgacgcgct ggacagactg cacaagccgg aagaagctgc agcgatgcgt cgcgacggtt
    16141 tgatgttaac gttgcagaac aacccaccac agtagttcct tctcacccgg aggcaagcac
    16201 ctccgggacc tttctgatgc ataaaaaaac gcctgctctt attacggagc aggcgttaaa
    16261 acaggtctgt atgacaacaa gtgggtgctt cactcaacgt tgtgtccatg gtgtctgatg
    16321 aggcataagc gacatctgtc agtggacgat aagcaccgta aacggctctg cgtcattccg
    16381 gagtttatga ggcactaagg cgaacataag agatggaatg agcatctacc cgtttattat
    16441 gccacagaga atcgggaaat aacatccctt aacacttgtt atgagataat tctgtaatcc
    16501 tctttgcttc ctgagtaata acttcctgag tgaatattta acctgagctt gatcctacac
    16561 atacttatta tgaatgataa aattcattca attaataaca catatattaa ttgccgttaa
    16621 aactaaaaaa agcatcaata atcaacgcga tataataaac ctgccttaca tatcaactgc
    16681 gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttctg gataaataaa
    16741 taatttattt ttgtcactat tatttatgta atcatcctgt cagggagagg gatctcaatt
    16801 atcaatgctt aattacgtca tcattttgat gacatgaaac cggagcattt tgcatggaat
    16861 tttcgttttc acccaaactt cttgttgttg ctgtcgccgc cgctcttcct ctcatggcca
    16921 gcgcagcaga taccccgtca actgccaccg cacgcaaagg ctttgccgga tacgatcacc
    16981 caaaccagta tccacagaac gctgcaattt attgaatttg cacgatcatg taggccggat
    17041 aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc cacaaaatgg ccgctttttt
    17101 acttattcac caccagcaaa tgtgcctgca taatgtcact ggccgggcga ccgtgcgcca
    17161 gcaaatcagc cattgcttta agatatggcg gtagatggcg gaaataacgc tgatacccgg
    17221 cacacaaata attcagcccc ggtttgccgc tggcatcgag catgaagcgg tgtttcgggc
    17281 agcctcccca gcacactttt aacacgttac aactgcgaca ctgcgccggt aattgcttaa
    17341 atttatcttc accaaacacc tgctgttgcg gggaatcgat catttctgca attgtttgct
    17401 gatgcatatt ccccagccga tattgcggat aaacatagtg atcgcaggcg taaacgtcgc
    17461 cgttgtgctc aacaatcacc gagcgcccac aggttggctg atgatggcaa accgcacccg
    17521 gcgcaccgac aaaattggca aacgcccatt cgatattcat cacgaaaatc ttgccgacgt
    17581 cgcgtttgat ccagtggtcg aatatcgcca ccagaaattc accaaattca tcggggcgca
    17641 ccgaccattc cgtcagttcc ccctgaatat caccaggcgc atgtagtttc agtccatcgc
    17701 tggcagctgt ttcatcagcc aggcgctcga ccaccggaat aaactggatg aattcgactc
    17761 ccgcatcgca caaaaaatca taaacctgca acggttgctg cgcgctggtg cggttgacgc
    17821 agaccagcac gttatagtcg acatgatgtt tttgcagcag cgtcagggca cgcatcacca
    17881 gcttatgcgt tggtctgcca cctttggtca cgcgatattg attgtggatc tcagccggac
    17941 cgtccagcga taacccaaca agaaaatgat tttctgccag aaatgcacac catttatcat
    18001 cgagcagcac gccgttagtc tggaagctgt tacttatctt cctgccagca ccgtatttcg
    18061 cctgtaactt tacggcacgg cggtaaaaat ccagcccgag taacgttggt tcgccgccct
    18121 gccaggtaaa agccacttcg ttttgcggtt cgctggcagc gatatagtga cggacatacg
    18181 cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg gattgttttt
    18241 cgagataaaa acagtagtca cagttcagat tacaatcgga accactcggt ttcgccatca
    18301 catgaaaagc acgcgttgga acctgttgca gcatggtcac tccttgcctg aagtcatcct
    18361 tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag ttattacggg cgtttattgc
    18421 gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg ggttcaaaat
    18481 tggtcggcga gagaggattc gaacctccga cccactggtc ccaaaccagt tgcgctacca
    18541 agctgcgcta ctcgccgaaa tactgctttt tgaattttta gttcaattct ttaaagtcgt
    18601 ggtgcgaggg gggggacttg aacccccacg tccgtaagga cactaacacc tgaagctagc
    18661 gcgtctacca attccgccac cttcgcattg tcacaacttc taataatggg gtggctaatg
    18721 ggattcgaac ccacgacaac tggaatcaca atccagggct ctaccaactg agctatagcc
    18781 accactacaa atcttgttac gcggtattac taccaccgca gctcaagcgc cgggactaaa
    18841 tggcgcgccc gacaggattc gaacctgaga cctctgcctc cggagggcag cgctctatcc
    18901 agctgagcta cgggcgctta gcgccgttgc gggggtggat aatacggact tcccacccct
    18961 ctgtctagtc cctttttaaa taaattgcgc gtttggttac ggtttgtgcg ttttgctgct
    19021 tattcctcca gtttatgcgc tttcccgtgg cgattaaggc caaaaacttt ataaatcgcc
    19081 gtcaccgcca gcataaagat gatgccaaca aacagcgaca tacgcgtatc ttcattaaag
    19141 tacatgccga tcaaaacgca aatcaggaat gccattgtta cgtagttggc ccacgggaac
    19201 aggattgagc ggaacggatg gctggcaatc gccgctttat gtgcacgccg aaaacgcagc
    19261 tggcttatca atatcacaaa ccatggcacc atccccggaa gcacgctggc actgtagacg
    19321 tagacaaaca cacgctgcgg attgggaatg atgtagttca ggcatgagcc aattagcaga
    19381 atagcaatag atactgccac acccgcaacg ggtacgccgt gacgggaaac tttcgccatc
    19441 gccgccggta actgacggtt tttcgccagt gcgtagagca tacgtccgca actgtacatg
    19501 ccgctgttac agccagagag cgcagccgtc agcaccacaa agttgataat gcccgccgct
    19561 gcggtaatac cgattttggc aaaagtcagt acgaacgggc tgccgttgct gcctatttca
    19621 ttccacggga agatggtgac gataacgaaa atcgcgccta cgtagaaaat caggatccgc
    19681 cacagcacct tgcctacggc actgcgcagc gtcacctgcg gattcttcgc ttcaccggca
    19741 gtaatgccaa tcagctccac gccctggtag gacgccacca caatacacag cgcggtcagg
    19801 aaccctttcc agccacccgc aaagaaaccg ccatgctctg tgagattgct aaaaccaatc
    19861 gactgcccgc cattgccaaa gccaaagaaa atcacgccca ggccaatgac aatcatcacg
    19921 ataatcgtgg tgactttgat catcgcgaac cagaactcga tttcgccgta caaccgcacc
    19981 gccgccagat tcgccaacgc caccagcgcc actgcgatca atgcgggtat ccactgcgcc
    20041 atctccggga accagaactg gacataaacg ccaatggcgg tgatttctga gatccccacc
    20101 gccatccaca taaaccaata agaccaggcg gtgagataac caaagaacgg gctcatataa
    20161 cgatgcgcat aaacggcgaa cgaaccggta accggttcga ggaacaacat ttcgcccatt
    20221 gaacgcatga tgaaaaagac gaatagcccg gcgatgatat aggccaacaa tacggatggc
    20281 ccggcccatt tcaaggtact ggcggccccc ataaacaggc cgacgccaat ggtgcccccc
    20341 agggcgatga gttcgatatg tcgagcttcc agcccacgct gtagctctgg tttgttatct
    20401 gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt taccggttat cgttatgggt
    20461 acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa
    20521 tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg agtgaccact ccgtcgcttt
    20581 acaaagagag gaaaatcaga ggttgccggt gtagtgccag cgtaaataac gcagcaaacg
    20641 aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg tagagccact ccagtcccag
    20701 cgtttgccag attttcggtg cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc
    20761 aacgcccata tacagcgcat ctggatgcac cagacggcag tcgcgcatga agatctcctg
    20821 ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa
    20881 cagcgcctga cgctgctcgg gtttaaaata accatcctga ctgccaacga tattcacatt
    20941 ccattggttg cgcagtttag cttcagtttg cgccagcact tcaggtttac cgcccacaag
    21001 aaataccggc gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga gatcggcacc
    21061 ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt actgaacgta caacgctgat
    21121 gccatccgca tatttaaatt cggcagcgtt aattaactcc ctgacctcgg cgttatcttc
    21181 aatagtcagc attttttcag cattaatggc aaccagcgtt ccctgcttaa gctgcccgtc
    21241 agcaaacaga taatcgaggg cgtgctgcat atcacgccaa ccaatcaact gtaagccacg
    21301 cagcgtatag gtcggtgccg tggtgttgtt attcattgtt atccttcaac ctgcgtccgg
    21361 agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca gtacaacagt
    21421 tttgcgatca tcagacatgc gccgaagacc acgataaaaa agactacgcg tgagacaaac
    21481 gaatccagcc cttcacgtgc cagcacgatc atattgaaga tcgccccaaa gcagaaactg
    21541 tgcaatatcg cagccttata gcgattagtc tcgcggttgc ccagctcata cagccagtcg
    21601 aaccatttga tgatcagtcc aaccacaatc gccccgagtg ggatgaacaa cgcgccgccc
    21661 atcaccacca gtgagcctat aagcgtaggc gagatcgcca gtccggagtg gttattcagc
    21721 acttcccagg taaagtagtt ggctgagttc agcaccatac tcgggcgacc cggccacagc
    21781 caggaaggga taaagacata gaaatcgcgg acaattggag ccaggccctg gaagtcgatg
    21841 ttgtcgtagt tctgcaacag caacgccaga ttctcccatg gcgagaaggt gtcgcgagtg
    21901 agatagagaa acgtatagaa cgcttcatcg ccgctcacat tcattccata gcgttttagc
    21961 gccagccaga acatgcccac aatccccagc acgcccgccg ccgccagcat ccacaacgaa
    22021 atccagccgc gaataatgcc aataaacagg aagatagcga atgcgatgat gatattggcg
    22081 cgagtgccgc cgacaatcat ataagtcagc aagccaaagg cgaccgtgct gacgaggaaa
    22141 aacagccacg ctttgctatc ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa
    22201 aagtaaaaga agcgttttaa cgccacgccg gagacttcac tggagaagat ctgactgtag
    22261 gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga agatgccgac gcttaccagc
    22321 gcgatgccca tcaggatcac ccacgtaaga ttggtctcca cgcggttcat ggtaaacagc
    22381 ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt gacatagtaa
    22441 accgcgtaga agcagcccgc agaaagcaac gcctgcaaca agatttctgg cggcgcgaca
    22501 ccaacatcaa agcgaaatac cagcacgctg gtcagcggga agccgaagaa aaaggtgagc
    22561 aaaaacagca atgaaaagaa gacattgaag ttaaagcgca cacggcgaaa ctcaaaccag
    22621 gtcagcgtgg caataaacag cgtgcagagc agccagacaa caaacaggcc actgaattgc
    22681 agcagactca tgcgacctcc ccggcggcaa tcgccaacgc ccgctgccag ccttgtagat
    22741 agttagggct aaagaaggca atggtgtttt tatccaccga cgccaactgg cgctgcgctt
    22801 cacgcacaat atcctcgttg agatcgtcgg tagtaaacag caccggcaaa tgctgttccg
    22861 tcatatcctg ccagaacgga ttttcccggt taagcacaca aggaatgccc gcctgaatca
    22921 gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca agatcgcact
    22981 gacgaagtag cgccagatag gcgtcaaatt ccagtttttc gctcaaaatt tgcagatttt
    23041 cttcgctgaa taactccagc cccgcctgac gaacttcctc aatgtacgct tcgttattag
    23101 gcggatatcc catcggcacc accactttta ccgtatcgcc aaattgctga tgaacggcgc
    23161 acaaggcagc aatatggtca ttgctgcggt caccggagtt ccccaccaga atggtcattt
    23221 tcccttcacg ttgccgatcg ttcgccatcg tattgagaga agggtccatc cgcgtcggga
    23281 agtacagcag ttcgccccgc acctttggat gcgttttggc aaaaaagctc aaatcaccgc
    23341 gggtggcaaa tacacagccg actcgctttt gcgccaggcg acgtagcggg taaaaaagct
    23401 tatatctcaa gccactggaa agctcataca ggtctgcccc ccagatatgc cagaaaaact
    23461 ggctgggctt aatgccacca ctcagcagag ccagccacag tttgggattg aactgaccgt
    23521 ggaagaaaaa acgctgctga cggttagctt ttgctttcgc gatgaccgct tccgccagcg
    23581 attttttccc agggaaaaat tgcacagaaa gcgccggaca gctatcactt aagccgtcgt
    23641 ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt cgcggccagc gcgtcattga
    23701 aaaaccgcaa aacggttcgg ttatggtgag ggatatccga tcccagtacg tgaatcagta
    23761 cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa aatagacgat
    23821 ataagttgcc atataggcct gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg
    23881 ggcaaatacc atcaataaag tgaactggct gacctccgcc agaatataaa accgcagtga
    23941 cgctttggcg atcaccagat aaccaaagac ataagcgccc acttttaaca catcacccac
    24001 caactgccag gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca gcagccagat
    24061 agcaaaatca cgcagcagcc agacggtaaa actcgccacc gccactgccg gtaagacgaa
    24121 tttcagcgat ttaaccactt cccgtgtgat atcgcgcttt tccgttagcc gcgacaacgt
    24181 gggcagcaaa tatacgctga acgatgccgt aataaattgc aggtaggcat cggaaatact
    24241 gctcacccct tgccagatcc ccacttcatc ccagctatac tgcgccgcca gcagtttacg
    24301 catcatgatg taagcaacag gcaaggtcac cgacgtaatc aacgccatga gcgtaaattt
    24361 gctcaactgc cctgccagac cgttatccca gctgggtttc agatagctta acgggatgac
    24421 accacgtttg atcaacatga tggcggcagg aatcaccacc agcgcgggaa tcagcgccag
    24481 acccagcaac gccccttcat aaccgcccaa acggtaactg acgtagtacg cgagaacgcc
    24541 aatcaagctg ccgacaatca gcgataacgc attacctgcg gcatcgcgaa agcctttcat
    24601 cagcgccagt aacaggttgc cccaggcaat ccccatttgc accagcgcca ctaaacgcac
    24661 cagcccctga tagtcggtat taccaaacaa tccctggctg attggcgcag ctgccagcac
    24721 aaaaaccagc gccatcagcg tagagaagcc aagtaccatc gctgatgaag tgccgaccac
    24781 gcggcgcagc tgttgcggat tatcatggta ctgggcaacg tatttggtta caccgttaaa
    24841 gatgccagcc ccggcaagca cgccgagcac ggtaatcaac tggcggaaat ttgccgccag
    24901 cccaagcccc gcaggaccaa atgacaccgc cagcaactta ccgaccagta acccggcacc
    24961 aatcttgacc agtgtactgg ccgccgtcca caaggacgct tttgccaacg acatatcagg
    25021 aaaagtagtt caacaaagtc gcaattaccg tacgctgatt gacgggcgac aggttgtaga
    25081 acagcggcag gcgcagcagg cgctcgctct ctttggtggt gtagcgatct tcaccgtgga
    25141 actcaccaaa gcgttccccc gcagggcaac cgtgcagcgg aatgtaatgg aacaccgcca
    25201 tgatttccgc ttctttcaga aagttaatca acgcgctccg gtcatcaata tcccgcagtt
    25261 taatgtagaa catatgcgcg ttctgcacgc agccatcggg aatcgacggc agctcgatac
    25321 gcccggcttt cgccagaggc gctaacgcat cgtagtagtt ttgccacagc gccagacgtt
    25381 gctggttgat acgatccgct gcttccagtt gcgcccacag gtacgcagct tgcagatcgg
    25441 acatcaaata gctggagcca atatcgcgcc aggtatattt atcgacctga ccacggaaga
    25501 actggctgcg gttagtgccc ttttcacgga tgatctcggc tcgttcgatt aacgctttat
    25561 cgttaatcag cgtcgcgccg ccttcaccgc ccgccgtgta gtttttggtt tcatggaagc
    25621 taaagcagcc aatatgacca atggttccca gtgcacgccc tttgtaagtg gacatcacgc
    25681 cctgagcggc atcttccacc acaaacagat tatgcttttt cgccaacgcc ataatggtgt
    25741 ccatttcgca ggccacgccc gcgtaatgca caggcacgat aacgcgcgtt ttgtcggtga
    25801 tcgccgcttc aatcagcgtt tcgtcgatat tcatggtgtc cgggcgaaca tccacaaaga
    25861 cgattttcgc gccacgcagc acaaaggcat tggcggtgga gacaaaggtg tagctcggca
    25921 tgatcacttc atcgccaggc tggatatcga gcagcagcgc cgccatctcc agcgaagcgg
    25981 tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg ttgctccagc cactgctggc
    26041 agcgacgggt aaaaccgcca tcgccacaca gtttgccgct acccattgcc gactgcatat
    26101 agtcgagttc ggttcccacc accggcggtg cgttaaatgg aatcatgtga tcacctgtat
    26161 aaccagtacg cggtgctttc tacattcgca ccactttgta tgtatcgttt aagcgcggcg
    26221 gtgttgccca tttgggtcgc cacccgcaaa gttgttttac cgcgagcata cgcccagttt
    26281 agcgccgttt gcatcagctc agcacctgca ccgcgtccag ccagcaggcc aattcgcgca
    26341 tctgtcgcat tgagttcccg taaagagaca tagccgcgaa tatcgccgga cgccgcacgt
    26401 aaaatcagac attgatgatc aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa
    26461 aagcgactgc tggcgtcagg cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg
    26521 gcgctggcta actgacgtaa tgcgggaata tcggtctctt gtgccactac agcaccacta
    26581 tcactgacat tgttcacggg tagtgccaaa tcaacttcac cttctaccag ggagaatccc
    26641 agctgttgca gggcatccag ttcacccgta tttgatgccg caattttggc ctgcacccgt
    26701 gaccacggcg ctaacacgtc tggcgtcagg agcggtgctt cagacgtaat gcgcacgatg
    26761 gcgctgttaa caccaaagaa ggcgttttcc caggttagtg gctcaatact ggcgcggacg
    26821 ggcacgaagt aactccagca gatattggcc gtagccagtt ttcgctaatg aactggcagc
    26881 acgcttcaca ccctcgtcat cgagccagcc gttacgccag gcgatctctt ccaggcaggc
    26941 aatcttaaag ccctggcgtt tttctactgt ctgcacaaag gtactggctt caatcaggct
    27001 gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg agcagttcaa cggtcaggtt
    27061 gcccgcctcg aggtacatct ggttgatgga ggtaatctcc agttcaccac gctccgacgg
    27121 cttcacctgc tttgcgtact ccacgacttt actgtcgtag aaataaagcc cggtcaccgc
    27181 ccagtttgac ttcggctgtt ttggcttttc ttccagcgag atagcgcgga aattgtcgtc
    27241 aaactccacc acgccaaagc gttccgggtc catcacctga tagccaaata ccgtcgcccc
    27301 ttccgtgcgc gccgcaacat gacgcagctt cggactgaag ccctgaccga agaagatgtt
    27361 atcgcccagc accagacaag aaggttcacc attgaggaag gtttcaccga tgataaaggc
    27421 ctgcgccaga ccgtccgggc tgggctgttc ggcgtattcc agctggatac cgaactcact
    27481 accatcgccc agcaggcgct ggaaataacc tttatcttcc ggcgtagtga tgatgagaat
    27541 ttcgcggata ccggccaaca tcagcaccga cagcggatag taaatcattg gcttatcgta
    27601 aatcggcaac agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc
    27661 gcccgccagg ataatacctt tcatgtttgc ctccgctgaa aattagcctt tcagacctaa
    27721 acgctcgccc tgatagctgc cgtcctgcac ctgcttccac cagctttcat tagccagata
    27781 ccactgcacc gttttacgca ttccactttc aaaggtttcc tgtggcagcc aaccaagttc
    27841 acgggcaatt ttcgaagcat caatggcata acgcagatca tgccccggac ggtcagcgac
    27901 aaaggtgatc aagtcacgat agtgcgccac gccgtgcggc ttgttcggag ccagttcttc
    27961 cagcagctcg caaatggttt ccacaacatc gagattctta cgctcgttgt gaccaccaat
    28021 attataggtt tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc
    28081 ttccacatac agccagtcac ggatttgctg cccgttgcca tataccggca gcggtttacc
    28141 cgccagcgcg ttgaggatca tcagcgggat cagtttttcc ggaaagtggt aaggaccgta
    28201 gttattcgag cagttggtga tcagcgtcgg cagaccgtag gtccgcagcc aggcgcgcac
    28261 cagatggtcg ctgctggctt ttgacgcgga ataagggctg ctcggcgcat acggcgtggt
    28321 ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg tggagatatg
    28381 atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta ttccagtaag cccgcgccgc
    28441 ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat
    28501 agaacggtca acatggcttt cggctgccag atgcatgaca cagtctggct gatgctcagt
    28561 gaatacgcgt gccagttctg cccgatcgca gatatcaact ttctcaaagg caaagcgctc
    28621 gctttgcgcg accggtgcca gcgacatcag gtttccggcg taggtcagct tatcgaccac
    28681 taccaccgcg tcgctcgttt cgttgatgat ataacgcacc agcgccgagc caataaaccc
    28741 ggcaccacct gttatcagaa tttttctcat cagcgccaga ctcctttggc atcgacgaca
    28801 tactgctgat ggacattgtc gccattgata actttgaact gactatgatc gaccagcatc
    28861 accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc
    28921 agttttttcg gcaactggtg gatgttaggc tcaacaacca gagtttcgcc gctatgccac
    28981 tgggcgatca gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc aatattcggt
    29041 ttaaacgcca gaccaaagca ggcgattttc agttcactgg cgcgtttatc ggtagcagcc
    29101 aggcaatcag ccaccgccgc tttcacctga tcgataaccc agaacggttt gtgatcgttc
    29161 acttcgcgcg cggtacggat aagccgcgcc tgctgggggt tctgtgccac gataaaccac
    29221 ggatcaacag caatgcagtg accgcccacg ccagggccag gctgaagaat attaacgcga
    29281 gggtgacgat tcgccaggcg aatcagttcc cagacgttaa tcccctgatc ggcacaaatc
    29341 agcgacaatt cattagcaaa agcgatattc acatcgcgga agctgttttc ggtgagctta
    29401 cacatttccg ccgtccgcga gttagtgacg acacactcac cttcgaggaa aattttgtac
    29461 agttcgctgg cgcgggtcga acaaaccggc gtcataccac caatcacgcg atcgttttta
    29521 atcagctcga ccataacctg gcccggtaac acgcgttccg ggcagtaagc aatgttgacg
    29581 tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg gacgcatctc tgctaaccat
    29641 tctgccatct tctcggttga ccccaccggc gaggtggatt caaggatcac cagcgcgcct
    29701 tttttcagca ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat atctggctca
    29761 tgatcgcctt taaacggcgt aggtacagca atcagccagg catccgcttc aactggcgtc
    29821 gtgctcgctc gtaaaaaacc gccttctacg gcagttttta ctacgctcgc caaatcaggt
    29881 tcgacgatat ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt gatatcgaca
    29941 ccaatcacct gtttttgccg tgaggcaaac gcagctgctg taggcagccc gatgtaaccc
    30001 agtccgataa cagaaatggt cgcaaaactc atagtgatat ccgattattt tttaacgctt
    30061 ccagaatgcg agagcatgcc tgaccatcac catacgggtt atgggcgcgg ctcatagctt
    30121 gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc tgcttatccg
    30181 tgcctaccag acgcaccgta cccgccgtca ccgcttccgg acgctcagtg gtatcgcgca
    30241 tcaccagcac cggtttcccc agcgaaggcg cttcttcctg aatgccgcct gagtcggtca
    30301 aaatcagcca ggcgtggttc atcagccaga caaacggtaa atactcctgg ggatcgatca
    30361 gaatgacatt tttcacatgc cccagaatgc gattgaccgg ctctctgacg ttcgggttga
    30421 gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt ggcgatgtct gccagcgcgt
    30481 ggcagatttc ttcaaagcca cgaccgaaac tttcacgcct gtgaccggtc accagaatca
    30541 tctttttatc ggggtcgata aacgggtaat ttgccgccag ttctgaacgc agcgtgtcgc
    30601 tgctcatcac ctgatcacgc acccataaca gtgcatcaat gactgtatta ccagtaatga
    30661 agattcggct atccgcaacg ttttcacgca gcaagttttg ccgggaagtt tcggttggag
    30721 agaagtgata catcgccaga tgcccggtca atgtacggtt agcctcttcc ggccacggcg
    30781 aatagagatc gcccgtgcgt aaaccagcct caacgtgacc aacaggaata cgctgataaa
    30841 acgccgccag gctggttgcc agcgtcgttg ttgtgtcgcc gtgaaccagc acgacgtctg
    30901 gtttgaactc ggcaagaata ggttttagcc cttccagaat ccgacaggtt atctctgtca
    30961 ggccctgtcc tggctgcatt atgttgagat cgtagtcagg tacaatggaa aagagtttca
    31021 gcacctgatc gagcatctcc cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag
    31081 gatcttttgc caacgcatgc accaacggag ccatcttgat ggcttccggg cgcgtaccaa
    31141 atacagtcag tactttcaca tcgattctct tcgaataagc ggcgagcgcc tttgcgctcg
    31201 ccgcagcagt gttgctattt cgagcaacgg cgggttaatg cgacaccagc cccgatcagc
    31261 cccccgacaa tgccccacat aatcatcagg aaggcacgac gtgggctatc gcgttttacc
    31321 ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac gcggatccag agttggacca
    31381 acattcaggg tgtttaacat ggcccgattc tgatcatagt cgagatcaaa ggccggaccg
    31441 acggcctgta aattttccag tcgagcctgg agcattggac gcccaagcag gaacatttct
    31501 gaatcaggta attcctcggc aggcacatct gtcgcactgc gcgaaatatt atgctgctca
    31561 gcaattttca gcgcctgctc aatgctgttc atccggcggt cgtagatggc tttcgccacc
    31621 tcttcctgac gcttcacctg agctttcatc tggatggtgc gtgccgccca tgcgcctttc
    31681 agctcatcat tcagatggct ggctgcacgc tggctggcaa aagcaacata ctgacgtaac
    31741 aggttattag cgtcaggcgc ggtttcggca ataagcttca cgctgtcatt gaccgcgcgg
    31801 gtaaagtctc cggggataaa ctggatgttg ttaatcattt catccagcaa cgccgcatcg
    31861 gctttgctgt tgcccaccat ccgctgcttg taatagtcgg tttgcagcca gaactctctg
    31921 cgggtatccc acgaggccag ttgcataaca aactctttat aggcttcgtc catgaccgat
    31981 ggttggtcgg cagaagccat gtttgaacgg gcatccaggt tacgcaaaaa ttgctgctgc
    32041 gagtaatatc cccccagcat attcaccgtt ggacgatcgg taatcgccgt cgcgctccac
    32101 tcttgtcgag caaaaaaagt atacgccagc gcgattaacg caaacgccag ccccatgcca
    32161 ataatccata gcttcccagc ccacaaggta cgaaacaacc cacgaatatc cagttcattt
    32221 tcagcgtctt cggccggttt cccaggcatt ggttgtgtca tcacatcctc atttatttgg
    32281 ttaaattggg gctgccacca cgatttctac gcagtctgcg ttttacgcgc ttaataaagc
    32341 gagcaacttt ccaggcacgc ttaatgcaat atccatagag gaagaatgct agcaaaaaga
    32401 gcaccagcat gacccactcc gggacaaaat gagaatattc tgccagcacg ccaatggaag
    32461 cgagcagtgc tgcggcaagg gtaatcagca caaacgcctg acgggaagta aacccggcac
    32521 gcatgatcaa atggtgaata tgctgacggt caggagagaa tgggctcatg cctttacgca
    32581 ggcgacggta cataatcgcc accatatcca ttagcggaat ggcgattatc cacaaagcgg
    32641 taaccgggct gatgggatgg gttttgccct gggtcgtttc gagcaggatc cagataacgg
    32701 taaaaccaat cagcgtactg cccgcatcac ccataaagac tttgtagcgg cgacccagga
    32761 taccaaggtt aagcatgatg tatggcagga tggcggcgat catcgcaaag caccagattg
    32821 cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac gagacgcagg
    32881 acaacccgcc cagcaagcca tcaatgccat caaccatgtt gaacgcatta atggccgccc
    32941 agacggcaaa tagcgtcagg aagtaaccaa acggtccgag caccatctcc caggagccaa
    33001 agatataacc caggctactg agataaagtt tgccgaacac catcataaca atgccaacag
    33061 cggcctgtat ggtggcacgg atttttacgc tgatatcaaa acggtcatcc agcgcgccaa
    33121 tgaaaacaag cacaccggca caagcgagat agagagatgc atgcggaata tagtaatcga
    33181 caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg agaggtatca
    33241 atccctggtg acgtttgcgg aagtttggtt tatccactaa accgactttt tttgccacct
    33301 tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat actgatgaga tcagtactca
    33361 ctgtcagtaa attcacaatg catgctctca gagaaaatta ttagcagaag tataaccacg
    33421 aagaccttta ttcagaaggg aaagtccgtt ccgaacccac cagtcctgtg caaaaattaa
    33481 ccatttgata tgcaaattgc ataacatgcc agcaaattcc aggaattttc ctaacatcgc
    33541 cagcgcggca ttaagattac agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt
    33601 tggcataaga caaatttatg agcgtttcat catttcgaag aaatcgtcat tggtcttggt
    33661 catggccagt ttattaatga ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat
    33721 tttgcgcagg atccacattt tctgcagttc ttcctgagtc gtgagcagct cttctttacg
    33781 ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt ttttcagcga tcttacgaga
    33841 gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg taaataactt cgtccatttt
    33901 agaaccggta tcgataagcg ccgtcgcgat aatggtcagg ctgccgccct cttccacgtt
    33961 acgcgccgca ccgaagaagc gtttcggacg atgaagggca ttggcgtcca caccaccggt
    34021 caacacttta cctgacgccg gaacaacagt gttgtaagcg cgcgccagac gagtgatgga
    34081 gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg cgtttggcct tctcgatcac
    34141 catttccgca acctgaacgt ggcgagatgc gggttcgtca aaggtagaag caacaacttc
    34201 accttttacc agacgctgca tctcggttac ttcttccgga cgttcgtcga tcagcagaac
    34261 catcagcaca caatccgggt ggttgtaagc aatgctctga gcaatgttct gcagcagcat
    34321 agttttaccg gctttcggcg gtgccacaat cagaccacgc tgaccacgac cgataggtga
    34381 tgccagatcc agtacgcgag cggttaaatc ttcagtagaa ccgttaccac gttccatacg
    34441 cagacgagag tttgcgtgca gcggggttaa gttctcaaag aggattttgt tgcgggcgtt
    34501 ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc gttcaccttc
    34561 tttcggcggg cgaatcttac cagagatggt atcaccagtg cggaggttga aacggcggat
    34621 ttggctaggg gaaacgtaga tgtcatcagg accggcgagg taggagctgt ctgcggaacg
    34681 gaggaaacca aatccatcct gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc
    34741 actctttgcg tgctgcttca ggatggcaaa aataatgtcc tgcttacgca tacgagccag
    34801 gttttccagc cccatatttt cgccgagagt gatcagctca gaaaccggcg tattctttaa
    34861 ttcggtaaga ttcatagtgg tgtgagttct taaacttggg gtaaatctcg aacttaatgt
    34921 tgtgaatggt atggcaggat catccatgcc tgtttacggt catcgactca tgtcttttcg
    34981 ctgcctggtc acaggagagt acgcagaact gaaacgacaa gacggattga gtgacgagcc
    35041 agaaatctgt tcacttcgca tttaagataa aacgggaagc gttggaaatt acaagattca
    35101 aacttaataa ggtatgttta atacgaagtc aacactaagt tagcatgact cacgccgggc
    35161 gtccagtttt tagcgacggg gcacccgaac atgaaattcc cttacgccag gttagcgtcg
    35221 aggaactctt tcaactgacc tttagacagt gcacccactt tggttgccgc cacttcaccg
    35281 tttttgaaca gcagcagagt cgggatacca cggatgccat atttcggcgc agtgccaggg
    35341 ttttgatcga tgttcagttt tgcaacggtc agtttgccct gatattcgtc agcgatttca
    35401 tccagaatcg gggcgatcat tttgcacgga ccgcaccact ctgcccagaa atcgacgagg
    35461 atcgccccgt ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt
    35521 ttatcgctca tatataactc cacaggaata agcctggcgt gttggtttcg ttgttggtgt
    35581 aacattaacc aactaaaggt tgactttatt tcaccggata cgctttcgta aagcaatagt
    35641 aagctgatat tctaccacac tatgagcaaa acacatttaa cagaacagaa gttttccgac
    35701 ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa aagggtttca taactgtacg
    35761 cccattcagg cactggccct tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg
    35821 caaaccggta ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta tcttctctct
    35881 catcctgcga ttgccgatcg caaggtgaat caaccacgtg ccttaattat ggcaccgacg
    35941 cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc tggcggaagc taccggcctg
    36001 aagctgggtc tggcttacgg tggtgatggc tacgacaaac agctgaaagt gctggaaagc
    36061 ggcgttgaca ttctgattgg cactacgggg cgtttaattg actacgccaa gcagaaccac
    36121 attaacctcg gtgccattca ggtggtggta ctggacgaag ccgatcgcat gtacgatctg
    36181 ggctttatta aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc
    36241 aacatgctgt tctccgccac gctttcgtac cgggtacgtg aactggcgtt cgagcagatg
    36301 aacaatgccg aatatattga agtggaaccg gaacagaaaa cgggccaccg tattaaagaa
    36361 gagcttttct acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa
    36421 gagtggccag acagagcgat tattttcgcc aataccaaac accgttgtga agagatctgg
    36481 ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa
    36541 aaacgtctgc gtattcttga tgaatttacc cgtggcgatc tggatattct ggttgccacc
    36601 gacgttgccg cgcgtggttt gcatattccg gcagtgacgc acgtctttaa ctacgattta
    36661 cccgatgact gtgaagatta cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc
    36721 ggtcactcta tcagcctggc gtgtgaagag tatgcattga atttgcctgc tattgagacc
    36781 tatattggtc actcaattcc ggtaagcaaa tacaatccgg acgcattgat gaccgatctg
    36841 ccaaaaccgc tgcgcctcac gcgcccacgt acaggcaatg gtccgcgtcg tactggcgct
    36901 ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata ctcaaaatca ttcaaactgt
    36961 atcaagatgg caagagaatg aatcccgatg agcatactgt agtaagtgat tcaggtgaaa
    37021 gaatgcagcc aacacagaga cagattgaag gatgaagagt atgggttcca cctcgtcgct
    37081 gtatgcagcc attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc
    37141 tggaagcatc cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa
    37201 tagcgaaaat gccctgtcta atgaagcaat ggagcgcggt tggcaatgtc tgcgcctgtt
    37261 tgctgaacgt ctgcaagata tccctccctc gcaaattcgc gttgtcgcta cggcgacatt
    37321 acgtcttgcc gtcaatgcgg gtgattttat tgccaaagcg caggaaatcc tcggttgtcc
    37381 ggtacaggtg atcagcggtg aagaggaagc acgtctgatt tatcagggcg ttgctcacac
    37441 aactggcggt gccgatcagc gcctggtggt ggatataggc ggtgccagta ctgaactggt
    37501 aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg
    37561 gctggaacgc tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa
    37621 agcggcacgc gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt
    37681 gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat
    37741 ggatgaacgc attaccctgg aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg
    37801 tcggctggag gaactggaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag
    37861 tggtctggcg atcctgatcg ccatttttac cgaactgaat attcagtgta tgaccctggc
    37921 gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg catctggccg tcgagcagga
    37981 tattcgcagc cgtacgctgc gtaatattca gcgccgcttt atgatcgata ttgatcaggc
    38041 gcagcgcgta gccaaagttg cggctaactt cttcgatcag gtggaaaagg aatggcatct
    38101 tgaagcaata agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag
    38161 cgttgacttt aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc
    38221 cggttttacc cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc agactaatcc
    38281 ggtcgatctc tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact
    38341 ctgccgttta ctccgcctgg ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc
    38401 agagatgaca ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct
    38461 aacccaacat ccgctgggta aagagattat tgctcaggaa agccagtggc agagctatgt
    38521 ccactggccg ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acgcatcttt
    38581 tccggccttg attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg
    38641 ctttgccctt tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc
    38701 agatcatcct gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagctcgccg
    38761 tactgacggc gttctttaca cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg
    38821 acataggcca gccgccgctc ctcatcgata ttatcttcat cgatgctgct ctggtgcggc
    38881 aaaaaccctt cttccatacc gaccatgtag acataaggaa actccagccc ttttgacgcg
    38941 tgcagagtca tcagttgcac ctgatccagc tcttcttcac tctcaccacg ctccatcatg
    39001 tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg
    39061 ccttccagca tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg
    39121 gcggctttcg ggctgggcga tgtttcgtac agccaggatt cataatccat gccgtggatc
    39181 agatcgcgca ccgcggcaat cggctcccgc tccgccagac gctggatttc tgccaaccag
    39241 tgagtaaagc gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata
    39301 tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc
    39361 agcgtcgccg ggccaatctc tctcttcggc gtgttaacga tacgcagaaa tgcgctgtca
    39421 tcgtccgggt tagtcagcac gcgcagataa gccagcaagt ccttgatttc aggacgagag
    39481 aaaaacgacg taccaccaga tattttgtat gggatgcggt tttgcatcag gaatttttca
    39541 aacacccgcg actgatggtt accgcgataa agaatggcgt aatctttgta ctgcgtttta
    39601 ttgacgaagt gatgggcgat cagctcgcca gtaacgcgct cggcttcatg ttcctcgtta
    39661 ttcgcgctta aaacttttag ctctgtgccg taacccagtt cggagaacag acgcttttca
    39721 aagacgtgcg gattattggc gatcaggatg ttcgccgctt tcaaaatacg tccggaagag
    39781 cggtagttct gctcaagctt aatcaccttc agcgccggaa aatcctgact cagcagcacc
    39841 aagttttgtg gacgtgcgcc gcgccaggag tagatcgact ggtcgtcatc gcccaccacg
    39901 gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt catactggct ggtgttggtg
    39961 tcctgatact catccaccag cagatagcga attttgttct gccagcgctc gcggacttct
    40021 tcattgcgtt gcagcagcaa cgtcggcaat aaaatcagat catcgaagtc gagaacattg
    40081 cacgctttca ggtgtgcatc atacagccca taacaatggg caaaaatacg gtcccgctcg
    40141 ccaatcgcac tggctgccgc ctgggacggt gttttgagat cgttcttcca gttagagatg
    40201 gtcgaaatca actgttgcag gagaactttg tcatcttcaa tcagcccttc ggtcaactct
    40261 ttgagcaacg ccagctgatc ggtatcgtca aacagcgaaa agttcgcctt catccccagc
    40321 gccgcatact cacgcttgat gatatctagc cccagcgtgt ggaaggttga gatcatcagc
    40381 ccacgcgcct ctttgcgacc cagcgtctgt ccgacacgct ctttcatctc gcgcgccgcc
    40441 ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaacc gcggatcaga
    40501 tgggcgattt tattggtgat cacacgagtt ttaccggaac ccgcgcccgc cagcaccagg
    40561 caggggccgg taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggtgta
    40621 ttgctcaatc ttcgaacggg gggaggattg tagcagaatc gcatttgaat gtgaccgcgt
    40681 gtaagccgct aaccggttgg ctgcggatgt ttaaatcaga gagagaagac cgaaaccgga
    40741 gttcatcgtg aatccattag acttagaaaa tatcgggtcg taacgttgtg ttattacttt
    40801 ttcagagttt actaaggaga ggtgtactcg tggcgattaa tttcagtcct aaggttggtg
    40861 aaatactgga atgcaacttt ggtaactatc ctgttagcca gaatggacag ttcagcacca
    40921 agtattacga tggccgcatt cctcctgaaa tgataaaaaa caggttagtt gtcgtgctca
    40981 acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact cgcgatcatg
    41041 acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttaacaat ggcctgcaat
    41101 tctttgatca gcagattcgt tgggccaaag ccgacctggt acaacaagtc agtcgaaacc
    41161 gattaaatag agcaaggact tacagagggt atcttaatca atgtttgcca catgagttgg
    41221 ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc ataaattgac
    41281 aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa gttttctccc
    41341 cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta ccagcccctc
    41401 cctgactgac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc tgacaattat
    41461 tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt gtaaaagaag
    41521 agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc ggcaagctgg
    41581 cgaagaaaca ctctatttgc ccgtcaggca aacgcggcgg tgatttaggt gaattccgcc
    41641 agggtcagat ggttccggcg ttcgataaag tggttttctc ttgtccggta ctggagccga
    41701 ccggcccgct gcacacccag ttcggatatc acatcattaa ggtgctgtac cgcaactaat
    41761 agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg tgggagaggg
    41821 tcggggtgag ggcatcagcg cgcacttaac ccgcaacagc aatacgtttc atatctgtca
    41881 tatagccgcg cagtttctta cctacctgct caatcgcatg gctgcgaatc gcttcgttca
    41941 catcacgcag ttgcgcgtta tctaccgcgc cttccggaat agctttaccc aagtcgcccg
    42001 gttgcagctc tgccataaac ggtttcagca acggcacaca agcgtaagag aacagatagt
    42061 taccgtactc agcggtatca gagataacca cgttcatttc gtacagacgc ttacgggcga
    42121 tggtgttggc aatcagcggc agctcgtgca gtgattcata atatgcagac tcttcaatga
    42181 tgccggaatc gaccatggtt tcgaacgcca gttcaacgcc cgctttcacc atcgcgatca
    42241 tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac tgcggcgcgg
    42301 tttcaaacgc ggttttgccg gtctcttcac gccaggtcag cagtttctta tcatcgttgg
    42361 cccagtccgc catcataccg gaagagaatt cgccggagat gatgtcgtcc atatgtttct
    42421 ggaacagcgg tgccatgatc tctttcagct gttcagaaag cgcataagca cgcagtttcg
    42481 ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc gcttcggtga
    42541 tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg ccctcttcca
    42601 ccagcttgtc gaagcacagc agcgagccag cctgcaacat accgcacagg atggtttgct
    42661 cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg cccgcacggt
    42721 gaccaccggt tgctgccgcc catgctttag cgatcgccat accttcgcct ttcggatcgt
    42781 tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac tcttcacgca
    42841 cttcggtgcc agggcatttc ggcgcaacca tgactacggt gatatcttta cggatctgct
    42901 cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg tctttcatca
    42961 gtggctgtac ggtgcgcact acatcagagt gctgcttgtc cggcgtcagg ttaaccacca
    43021 gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt tcggtcgctt
    43081 tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag gagatatcga
    43141 gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag ccgacgataa
    43201 ccactttttt atcctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc cccataaagc
    43261 gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag tagttagcca
    43321 tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg agattgaatt
    43381 cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca cattgcaatt
    43441 tttgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa aaccttcctg
    43501 catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt tagcccatcc
    43561 acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct atttgtgcgc
    43621 gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt cgcccagcaa
    43681 acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc gtcgctctct
    43741 ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc gccgattctg
    43801 gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg tgatgcggca
    43861 gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg gaaaccggag
    43921 acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt gctgattgcg
    43981 cccgcgctgc cctgcccggt gcgtaaccag gtttcggcag agaagccgga ctggtctaca
    44041 gtgccgttta tcatggccga tcagggaccg gtgcgtcgcc gcattgaact gtggtttcga
    44101 cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga agcgatggta
    44161 tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtagtgct ggaaaacagc
    44221 cccgaaccgg tgcgtaaccg cgtgatgatt ttagagcgca gcgatgaaaa aacgccgttt
    44281 gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga ggcgttctgg
    44341 aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa gcgaagcgct
    44401 atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    44461 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    44521 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    44581 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    44641 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    44701 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    44761 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    44821 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    44881 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    44941 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    45001 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    45061 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcgcgcac
    45121 atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg tgatgatgtc
    45181 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgctacgcc
    45241 ttcagcaaat agccctacgc gcggcagatc aaccggatga cccgcatcca gcgctgcttt
    45301 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca tcagttgttt
    45361 gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    45421 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    45481 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    45541 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    45601 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    45661 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    45721 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    45781 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    45841 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    45901 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    45961 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    46021 ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    46081 ttgtcacctc gagcgtcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    46141 aactgaatgc cacggttcgg gatgtctata gcgatcaggt caccatcttc aatcaggcca
    46201 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    46261 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    46321 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    46381 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    46441 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    46501 gttttaacga tgcagccgtt ttctgcgaaa ttaccgtaga gcaccgccag gccgccgtct
    46561 ttgctgtagg cgtgttccag cgagcggata cagccattgg cgcgatcgtc atcgagagaa
    46621 tcccaacggc aatcctgcga gaatgcctgt gtggtacgaa tgcctgcagg acctgcgcgg
    46681 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagcgtttgc
    46741 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    46801 tcgccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttctgg
    46861 gtgctcggcg caactttaca cagctgtgga accttgcggg aaagcttatc gatatcactc
    46921 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac ggtgttagtc
    46981 gatccaccca tcgcgatatc cagcgtcatg gcgttttcaa acgccgcttt gctagcaata
    47041 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    47101 cgtttaccgg cattgaggaa caactgctta cggtcggcgt gggttgccag cagcgagccg
    47161 ttgcccggct gcgacagacc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    47221 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    47281 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    47341 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    47401 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    47461 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    47521 tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    47581 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    47641 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    47701 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    47761 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    47821 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    47881 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    47941 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa cctgaatacc
    48001 atctacactg cgcactggcg tgatttctgc cgcagtaccg gacataaaca cttcatccgc
    48061 caggtacagg gattcgcgcg acagaacctg ctcacgcact tcaattccca gctctttcgc
    48121 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    48181 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    48241 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    48301 cagggaagag agatagttac caccggcttt tgccgcggtt gggatggtgt ttggtgctgc
    48361 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    48421 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    48481 acccatgcca acatcaccaa cgaagatcag cggacggata taggcgctgg tgagattgtt
    48541 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg aaaccgggaa
    48601 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    48661 cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc catagtgcag
    48721 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    48781 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    48841 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    48901 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    48961 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    49021 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    49081 gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg gcaccagcgg
    49141 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    49201 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    49261 gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt cgctgtatcg
    49321 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    49381 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    49441 gaaagagccg tcaccggaga tacagacgac agtatcgttc ggtcgtgcga cttgtgcgcc
    49501 aaccgccgct ggtaaaccga aacccatggt gcctaagccg ctggaggtaa tgaaattttc
    49561 cgggcgagtg tgtgcgatgt gctgcgcggc ccacatctgg tgctgcccca catctgtggt
    49621 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gcgcgtagat
    49681 agcgtcaccg ggatggtcgt aacgccaggt atgttcatca cgcagctgcg cgcagtattg
    49741 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    49801 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    49861 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    49921 tgcacccacg gcgatcagca agtcgcactc ctgcaccgcg aagttcgccg ctttggtgcc
    49981 atgcattccc agcatgccca ggtagtacgg ataatctgct tcaactgcgc ccagcccttt
    50041 cagcgtgcag gtggcaggca tttttgtggt agcgagaaat tctcgtaaag caggaactgc
    50101 ctgcgccata cccacgccac caccaacgta cagcatcggt ttttgcgctt ttgccagcat
    50161 ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    50221 ccacggttcc aggtcaccgc tggctagctg gatatctttt gggatatcga ccagaaccgg
    50281 accaggacga cctgagctgg caacgtcgaa tgctgcagcc atgatgcgcg gcaactcttc
    50341 cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    50401 ttcctgaaat gcgtcggtgc cgataaaagg tgcggacact tgaccggtga tggcaacgac
    50461 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    50521 agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga tagccgccat
    50581 tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat acaatgcatc
    50641 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    50701 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    50761 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    50821 aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct
    50881 ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg ctaatcacga
    50941 ccaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct tgttcgaagg
    51001 aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg ggcctatttt
    51061 tatgatggct ttaataattt caatagaaat tgttgttttt taaaaagaaa atagtgagtg
    51121 aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc ctttgtccgc
    51181 tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg cacaaataaa
    51241 caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat cttatgcaca
    51301 taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc gcagccctgg
    51361 gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta cccggcttaa
    51421 cgatggtagg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg cgcagcgcca
    51481 ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt gctccagccg
    51541 atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg ctggcggcct
    51601 cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa ctggctctta
    51661 caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct attaagtcgg
    51721 gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt aacggtgaag
    51781 gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt aagcacgctc
    51841 tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat ctcagtgatg
    51901 ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc gggcacaacc
    51961 ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt attaatggcc
    52021 tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta agtctggtaa
    52081 atgctgagtc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct catcacagtg
    52141 cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa atttcgctgg
    52201 cgcataacgg cgtgcttttt cttgatgagt tacctgagtt tgaacggcgt acactggatg
    52261 ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacacga gcaaaaataa
    52321 cctatccagc ccgtttccag cttgtcgcgg cgatgaatcc cagccctacc ggacattatc
    52381 agggaaacca taaccgctgc acgccagaac agacattacg ttatctcaac cggctctcgg
    52441 ggccctttct cgaccgcttc gatctctcac tggagatccc attaccaccc cccggcattt
    52501 tgagtaaaac ggtagtgccg ggagaaagca gcaccaccgt taaacaacgt gtaatggctg
    52561 ccagagagcg ccaatttaag cggcagaata agttgaacgc ctggctggat agtccggaaa
    52621 tacgccaatt ctgcaagctt gagagcgaag atgcgcagtg gctggaagaa acgctgatcc
    52681 atctggggtt atcgattcgt gcctggcagc ggttattgaa agttgcacga accattgctg
    52741 atattgatca gtccgacatt atcacacgtc agcatttgca ggaggcagtt agctatcgtg
    52801 ccattgaccg tttgctcatc catctgcaaa aactactgac ataaaaaaag ggcatttcgc
    52861 cctttttatt aatcgtcaga atcggtgtag tcttcagcac cttcaacctg cggtttaccg
    52921 ccggaaaggg tgtgaaaacg ttttggacgc ttgatacgcg tcatatactt ggaccacacg
    52981 cgttctgctt ctgtcactgg ctcacgttcg ccacggcata ctgctacgaa gagtttctct
    53041 tcctcggtaa ccggctcgcg tttgccaaga tccaactcat tgaaggcata accatgacgc
    53101 tcaagcagtt gtgcctcttt gatggtgaaa tcaccatgac gagagaatcc acgtggataa
    53161 tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct gcacgctcct
    53221 aattctttga ccgagctagt tatggcgcgg agtattagtt acgcttgaca gagtgtaaaa
    53281 caaaacattt aaatcataac gacaaataat tttgcggaga gcactgtgga tacggaattg
    53341 ttaaaaactt tcctggaagt tagccgaacg cgtcactttg gtcgagcggc tgaatcgctc
    53401 tatctgaccc agtcagcagt gagctttcga atcagacaac tggaaaatca actgggtgtg
    53461 aaccttttca cccgccacag aaacaatatc cgtttaaccg ctgccggtga aaaactactg
    53521 ccttatgcag aaacgctcat gagcacctgg caggccgccc gtaaggaggt ggcgcatacc
    53581 tcacgacata acgagttttc tatcggtgcc agcgcctcat tgtgggaatg tatgcttaat
    53641 cagtggctgg gacgtttgta tcaaaatcag gatgcccata caggcttaca gttcgaagcg
    53701 cgaattgccc aacggcagtc tctggtaaaa cagctgcatg aacgccagct tgatcttctt
    53761 attaccactg aagcgcccaa aatggacgaa tttagtagtc agttgctggg gtatttcact
    53821 ttagcgcttt ataccagtgc cccttctaaa ctaaagggag atcttaacta tctgcgactg
    53881 gagtgggggc cagattttca acagcatgag gcaggtttga tcggtgctga cgaagtgccc
    53941 attctgacaa ccagttctgc tgaactggca cagcaacaga ttgcgatgct taatggttgc
    54001 acctggctac ccgtcagctg ggcgcgtaaa aaaggcggcc tgcataccgt tgtcgatagc
    54061 acaacacttt cacggccgct ttatgccata tggctgcaaa atagcgataa aaatacgttg
    54121 attcgcgatc ttttgaaaat taacgtgctg gatgaagtgt attaatatga atggctggca
    54181 aggatgccgg tagaaggatt tacttcggag agggttattt cagataaaaa aaatccttag
    54241 ctttcgctaa ggatgatttc tggcaggggc ggagagactc gaactcccaa cacccggttt
    54301 tggagaccgg tgctctacca attgaactac gcccctaatt agggtggcgg aacggacggg
    54361 actcgaaccc gcgaccccct gcgtgacagg caggtattct aaccgactga actaccgctc
    54421 caccgaattc ttttacaacc accggtttta tgaccggctt
//
LOCUS       DAZFRN010000011        54341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFRN010000011 DAZFRN010000000
VERSION     DAZFRN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.011"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..663
                     /locus_tag="ACHKUB_000644"
     CDS             <1..663
                     /locus_tag="ACHKUB_000644"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO6653706.1"
                     /translation="VLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKGIYI
                     AAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISALEDI
                     IKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSFMPQ
                     VFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVIHKM
                     IL"
     gene            673..1869
                     /gene="yaiP"
                     /locus_tag="ACHKUB_000645"
     CDS             673..1869
                     /gene="yaiP"
                     /locus_tag="ACHKUB_000645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6653707.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1847..2428
                     /locus_tag="ACHKUB_000646"
     CDS             1847..2428
                     /locus_tag="ACHKUB_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6653708.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2430..3203
                     /gene="yaiO"
                     /locus_tag="ACHKUB_000647"
     CDS             2430..3203
                     /gene="yaiO"
                     /locus_tag="ACHKUB_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO6653709.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3388..3663
                     /gene="frmR"
                     /locus_tag="ACHKUB_000648"
     CDS             3388..3663
                     /gene="frmR"
                     /locus_tag="ACHKUB_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO6653710.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3698..4807
                     /gene="frmA"
                     /locus_tag="ACHKUB_000649"
     CDS             3698..4807
                     /gene="frmA"
                     /locus_tag="ACHKUB_000649"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO6653711.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4900..5733
                     /gene="fghA"
                     /locus_tag="ACHKUB_000650"
     CDS             4900..5733
                     /gene="fghA"
                     /locus_tag="ACHKUB_000650"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6653712.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5858..6397)
                     /gene="yaiL"
                     /locus_tag="ACHKUB_000651"
     CDS             complement(5858..6397)
                     /gene="yaiL"
                     /locus_tag="ACHKUB_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO6653713.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6499..7710)
                     /gene="mhpT"
                     /locus_tag="ACHKUB_000652"
     CDS             complement(6499..7710)
                     /gene="mhpT"
                     /locus_tag="ACHKUB_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO6653714.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(7985..8998)
                     /gene="mhpE"
                     /locus_tag="ACHKUB_000653"
     CDS             complement(7985..8998)
                     /gene="mhpE"
                     /locus_tag="ACHKUB_000653"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO6653715.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(8995..9945)
                     /gene="mhpF"
                     /locus_tag="ACHKUB_000654"
     CDS             complement(8995..9945)
                     /gene="mhpF"
                     /locus_tag="ACHKUB_000654"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO6653716.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9942..10751)
                     /gene="mhpD"
                     /locus_tag="ACHKUB_000655"
     CDS             complement(9942..10751)
                     /gene="mhpD"
                     /locus_tag="ACHKUB_000655"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO6653717.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10761..11627)
                     /gene="mhpC"
                     /locus_tag="ACHKUB_000656"
     CDS             complement(10761..11627)
                     /gene="mhpC"
                     /locus_tag="ACHKUB_000656"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO6653718.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11645..12589)
                     /gene="mhpB"
                     /locus_tag="ACHKUB_000657"
     CDS             complement(11645..12589)
                     /gene="mhpB"
                     /locus_tag="ACHKUB_000657"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO6653719.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12591..14255)
                     /gene="mhpA"
                     /locus_tag="ACHKUB_000658"
     CDS             complement(12591..14255)
                     /gene="mhpA"
                     /locus_tag="ACHKUB_000658"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO6653720.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGGSFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQILGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14446..15279
                     /gene="mhpR"
                     /locus_tag="ACHKUB_000659"
     CDS             14446..15279
                     /gene="mhpR"
                     /locus_tag="ACHKUB_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO6653721.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15356..16438
                     /gene="lacI"
                     /locus_tag="ACHKUB_000660"
     CDS             15356..16438
                     /gene="lacI"
                     /locus_tag="ACHKUB_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO6653722.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16561..19635
                     /gene="lacZ"
                     /locus_tag="ACHKUB_000661"
     CDS             16561..19635
                     /gene="lacZ"
                     /locus_tag="ACHKUB_000661"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO6653723.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19687..20940
                     /gene="lacY"
                     /locus_tag="ACHKUB_000662"
     CDS             19687..20940
                     /gene="lacY"
                     /locus_tag="ACHKUB_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO6653724.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTVNNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21019..22200)
                     /gene="cynX"
                     /locus_tag="ACHKUB_000663"
     CDS             complement(21019..22200)
                     /gene="cynX"
                     /locus_tag="ACHKUB_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO6653725.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQVVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22206..22676)
                     /gene="cynS"
                     /locus_tag="ACHKUB_000664"
     CDS             complement(22206..22676)
                     /gene="cynS"
                     /locus_tag="ACHKUB_000664"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO6653726.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22707..23366)
                     /gene="cynT"
                     /locus_tag="ACHKUB_000665"
     CDS             complement(22707..23366)
                     /gene="cynT"
                     /locus_tag="ACHKUB_000665"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO6653727.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23476..24375
                     /gene="cynR"
                     /locus_tag="ACHKUB_000666"
     CDS             23476..24375
                     /gene="cynR"
                     /locus_tag="ACHKUB_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO6653728.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24415..25698)
                     /gene="codA"
                     /locus_tag="ACHKUB_000667"
     CDS             complement(24415..25698)
                     /gene="codA"
                     /locus_tag="ACHKUB_000667"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO6653729.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25688..26947)
                     /gene="codB"
                     /locus_tag="ACHKUB_000668"
     CDS             complement(25688..26947)
                     /gene="codB"
                     /locus_tag="ACHKUB_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO6653730.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27184..29070)
                     /gene="prpE"
                     /locus_tag="ACHKUB_000669"
     CDS             complement(27184..29070)
                     /gene="prpE"
                     /locus_tag="ACHKUB_000669"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO6653731.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29110..30561)
                     /gene="prpD"
                     /locus_tag="ACHKUB_000670"
     CDS             complement(29110..30561)
                     /gene="prpD"
                     /locus_tag="ACHKUB_000670"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO6653732.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(30595..31764)
                     /gene="prpC"
                     /locus_tag="ACHKUB_000671"
     CDS             complement(30595..31764)
                     /gene="prpC"
                     /locus_tag="ACHKUB_000671"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO6653733.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(31809..32699)
                     /gene="prpB"
                     /locus_tag="ACHKUB_000672"
     CDS             complement(31809..32699)
                     /gene="prpB"
                     /locus_tag="ACHKUB_000672"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO6653734.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            32938..34524
                     /gene="prpR"
                     /locus_tag="ACHKUB_000673"
     CDS             32938..34524
                     /gene="prpR"
                     /locus_tag="ACHKUB_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO6653735.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(34622..34897)
                     /gene="yahO"
                     /locus_tag="ACHKUB_000674"
     CDS             complement(34622..34897)
                     /gene="yahO"
                     /locus_tag="ACHKUB_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO6653736.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35044..35715
                     /gene="yahN"
                     /locus_tag="ACHKUB_000675"
     CDS             35044..35715
                     /gene="yahN"
                     /locus_tag="ACHKUB_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO6653737.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(35732..35977)
                     /gene="yahM"
                     /locus_tag="ACHKUB_000676"
     CDS             complement(35732..35977)
                     /gene="yahM"
                     /locus_tag="ACHKUB_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO6653738.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRGRPMKKAHTGES
                     GLKWEAQDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(36390..37205)
                     /gene="yahL"
                     /locus_tag="ACHKUB_000677"
     CDS             complement(36390..37205)
                     /gene="yahL"
                     /locus_tag="ACHKUB_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO6653739.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(37448..38497)
                     /gene="yahK"
                     /locus_tag="ACHKUB_000678"
     CDS             complement(37448..38497)
                     /gene="yahK"
                     /locus_tag="ACHKUB_000678"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO6653740.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(38584..39540)
                     /locus_tag="ACHKUB_000679"
     CDS             complement(38584..39540)
                     /locus_tag="ACHKUB_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6653741.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(39537..40508)
                     /locus_tag="ACHKUB_000680"
     CDS             complement(39537..40508)
                     /locus_tag="ACHKUB_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6653742.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40501..41985)
                     /locus_tag="ACHKUB_000681"
     CDS             complement(40501..41985)
                     /locus_tag="ACHKUB_000681"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6653743.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42034..43017)
                     /locus_tag="ACHKUB_000682"
     CDS             complement(42034..43017)
                     /locus_tag="ACHKUB_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6653744.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43271..43681)
                     /locus_tag="ACHKUB_000683"
     CDS             complement(43271..43681)
                     /locus_tag="ACHKUB_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO6653745.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(43980..45362)
                     /gene="yahJ"
                     /locus_tag="ACHKUB_000684"
     CDS             complement(43980..45362)
                     /gene="yahJ"
                     /locus_tag="ACHKUB_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO6653746.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(45372..46322)
                     /gene="yahI"
                     /locus_tag="ACHKUB_000685"
     CDS             complement(45372..46322)
                     /gene="yahI"
                     /locus_tag="ACHKUB_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO6653747.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46465..47883)
                     /gene="yahG"
                     /locus_tag="ACHKUB_000686"
     CDS             complement(46465..47883)
                     /gene="yahG"
                     /locus_tag="ACHKUB_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6653748.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(47883..49430)
                     /gene="fdrA"
                     /locus_tag="ACHKUB_000687"
     CDS             complement(47883..49430)
                     /gene="fdrA"
                     /locus_tag="ACHKUB_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6653749.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49420..50283)
                     /gene="yahE"
                     /locus_tag="ACHKUB_000688"
     CDS             complement(49420..50283)
                     /gene="yahE"
                     /locus_tag="ACHKUB_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6653750.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50323..50928)
                     /gene="yahD"
                     /locus_tag="ACHKUB_000689"
     CDS             complement(50323..50928)
                     /gene="yahD"
                     /locus_tag="ACHKUB_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO6653751.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            51186..51683
                     /gene="yahC"
                     /locus_tag="ACHKUB_000690"
     CDS             51186..51683
                     /gene="yahC"
                     /locus_tag="ACHKUB_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6653752.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGVISEYGGQKLSEATT
                     KRDGY"
     gene            51775..52707
                     /gene="yahB"
                     /locus_tag="ACHKUB_000691"
     CDS             51775..52707
                     /gene="yahB"
                     /locus_tag="ACHKUB_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6653753.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(52749..53837)
                     /gene="pdeL"
                     /locus_tag="ACHKUB_000692"
     CDS             complement(52749..53837)
                     /gene="pdeL"
                     /locus_tag="ACHKUB_000692"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO6653754.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            54157..>54341
                     /locus_tag="ACHKUB_000693"
     CDS             54157..>54341
                     /locus_tag="ACHKUB_000693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653755.1"
                     /translation="MSKGALYEFNNPDQLKIPLPHKHIASTFNDIMSKDVGYAYVSLL
                     YACPLKTHPLRLNPFSK"
BASE COUNT        12972 a        14730 c        13678 g        12961 t
ORIGIN      
        1 gttttagatt cagccctcct ttcttcggca aataaaagaa agggtatcct tgctattggc
       61 gcacatcctg acgatataga attaggctgt ggcgcatcgc ttgctcgtct tgcgcaaaaa
      121 ggaatttata tcgcagccgt ggtgatgact accggcaact ctggcacaga tggaataatc
      181 gatcgccatg aagaatcgcg caacgcccta aagatattag ggtgccacca aactattcat
      241 cttaattttg ctgacacccg cgctcattta cagctcaatg atatgatttc cgccctggaa
      301 gacatcatta aaaatcaaat tccttctgat gttgaaatca tgcgggtata taccatgcat
      361 gatgccgacc gccatcagga tcatctcgct gtttatcaag cttcaatggt tgcctgccgc
      421 actattccac aaattctcgg ctacgaaacc ccaagtacct ggctttcatt tatgcctcag
      481 gtttttgaat ccgttaaaga agaatatttc acagtcaaac ttgctgcatt aaaaaaacat
      541 aaaagccagg aacgacgcga ttatatgcgt catgatcgcc tgcgtgcagt tgcacaattt
      601 cgcgggcaac aggtcaatag cgatctgggt gaaggctttg taattcataa aatgattctt
      661 tgagcaaatt gcatgaaaac ctggatattt atctgtatgt ccatagcaat gttgctatgg
      721 tttttaagta cgctaagacg taaacccagt caaaagaaag gctgtattga cgccattata
      781 cctgcgtata acgaaggccc gtgtctggcg cagtcactgg ataatctact gcgtaaccct
      841 tatttttgcc gggtaatttg cgttaacgac ggctccacgg acaataccga agcggtcatg
      901 gcggaagtca aacgcaaatg gggcgaccgc tttgttgccg tcacgcaaaa aaataccggt
      961 aaaggtggtg cgctgatgaa tggcctcaat tatgccacct gcgaccaggt ttttttaagt
     1021 gatgccgaca cctatgttcc gcccgatcaa gacggaatgg gctatatgct ggcagaaatt
     1081 gagcgcggtg ctgatgccgt aggcggcatt ccctctactg cgttgaaagg cgcggggctg
     1141 ttaccgcaca tccgcgcgac cgtaaagttg ccgatgattg ttatgaagcg cacgctacag
     1201 cagctcctgg gcggcgcacc gtttattatc agcggtgcct gcggaatgtt ccgcactgat
     1261 gtattgcgta agttcggttt ctcagatcgt actaaagtcg aagaccttga tctcacctgg
     1321 acattagtgg caaacggcta ccgtattcgg caggcgaatc gctgcatcgt atacccacag
     1381 gaatgcaaca gcccgcgtga ggagtggcgt cgctggcggc gttggattgt gggatacgcg
     1441 gtctgtatgc gcctgcataa aagactttta tttagccgct tcggtatctt cagtatattt
     1501 cctatgctgt tggttgtgat ttatggcgtt gggatttatc tcactacctg gtttaatgaa
     1561 ttcatcacca ccgggccgca tggtgtggtg ttggcaatgt ttccgcttat ctggatcggc
     1621 gtagtttgtg ttattggtgc ttttagcgcc tggtttcatc gttgctggtt gttggtgcct
     1681 ttagcgccgc tttccgttgt gtatgtatta ttagcttatg ccatctggat tatttatgga
     1741 cttattgcct tttttactgg acgcgaacct cagcgcgaca aacccacccg ctattccgca
     1801 ctggtggaag cgtcaaccgc ttattcccaa ccttctgtca caggaactga aaaactatct
     1861 gaagcttaat gttaaagaga aaaatattca gattgcagac caggtaatta ttgatgaaac
     1921 tgcaggtgaa gttgttatcg gcgcgaatac ccgtatttgt catggtgccg ttattcaggg
     1981 tccggtagtg attggcgcaa actgcctgat aggtaattat gcgtttattc gtcctggcac
     2041 aataatcagc aatggtgtaa aaattggttt tgccaccgaa attaaaaatg cggttattga
     2101 agcggaagca acgattggtc cgcaatgttt tattgccgac tcggtagttg caaaccaggc
     2161 atatttgggc gcacaagtac gtaccagtaa tcatcgtctg gatgaacagc ctgtgtctgt
     2221 tcgaactcca gagggaatta tcgctaccgg atgcgataaa ttaggttgtt atatcgggca
     2281 gcgttcacgc cttggcgtac aagttataat tttgcctggg cgaattattt ccccgaacac
     2341 gcaacttggc ccgcgcgtga ttgtagaacg taatttacct gccggaactt attcactccg
     2401 acaagaactt atccgtacag gagattaaaa tgataaaacg gacgttatta gcggcggcca
     2461 tttttagcgc attgcccgct tatgccgggt taacttccat taccgcgggc tacgatttta
     2521 ccgattattc tggcgatcat ggcaaccgta atttagcgta tgctgaactg gtggcgaaag
     2581 ttgaaaacgc aacgctgctt tttaatcttt cccaggggcg tcgtgattat gaaactgaac
     2641 atttcaatgc cactcgcggt cagggtgcgg tctggtataa atggaataac tggctgacaa
     2701 cccgaacggg tattgccttt gcggataata cgccggtctt tgcccgccag gattttcgtc
     2761 aggatattaa cctggccctg ttgccaaaaa cgcttttcac gaccggttat cgctacacta
     2821 aatattacga tgatgtcgaa gtcgatgcct ggcaaggcgg cgtatcactc tatactggcc
     2881 cggtgatcac cagctaccgc tatacccatt atgactccag cgatgcaggt ggtagttata
     2941 gcaatatgat ttccgtgcgt ctgaatgacc cgcgcggcac tggttatacg caactatggc
     3001 taagccgcgg aacaggcgct tacacctatg actggacgcc agaaacacgc tacggcagca
     3061 tgaagagcgt cagtttgcaa cgtattcaac cgctgactga gcaacttaat ctcgggctga
     3121 cggcaggtaa agtgtggtac gacaccccaa ccgatgatta taacggtctg caacttgcag
     3181 cccatctgac ctggaaattc tgattccttc tgccgcccgc tatccggggc ggccttccct
     3241 gccgattagc cccccctttc ctctttgttt tccgaccaca ttcaccggat aaattttatt
     3301 ctccagtgtt atatactata ggggggtatg cattgacata tagcataccc ccctatagta
     3361 tattgcgtgc agatgatgag gtgcgaaatg cccagtactc cggaagagaa gaaaaaggtc
     3421 cttactcgag ttcgtcgtat tcgagggcag attgatgctc tggaacggtc gctggagggt
     3481 gatgccgaat gccgtgccat actccaacag atcgctgccg ttcggggcgc ggctaacggg
     3541 ctgatggcag aagtgcttga aagccatatc cgggaaacgt ttgaccgaaa tgactgctac
     3601 agccgcgaag tcagccaatc cgttgacgac actattgaac tggttcgagc ctatcttaaa
     3661 tagctgaatc tattaccata ttgaggaaga gcgagagatg aaatcacgtg ctgccgttgc
     3721 atttgctccc ggtaaaccgc tggaaatcgt tgaaattgac gttgcaccac cgaaaaaagg
     3781 tgaagtgctg attaaagtca cccataccgg cgtttgccat accgacgcat ttaccctctc
     3841 tggggatgac ccggaaggtg tattcccggt ggttctcggt cacgaagggg ccggcgttgt
     3901 ggttgaagtc ggtgaaggcg taaccagcgt caaacctggc gaccatgtga tcccgcttta
     3961 caccgcggag tgcggcgagt gtgagttctg tcgttctggc aaaactaacc tctgtgttgc
     4021 ggttcgcgaa acccagggta aaggcttgat gccagacggc accacccgtt tttcttacaa
     4081 cgggcagccg ctttatcact acatgggatg ctcaacattc agtgaataca ccgtggtcgc
     4141 ggaagtgtct ctggccaaaa ttaatccaga agcaaaccat gaacacgtct gcctgctggg
     4201 ctgtggcgtg accaccggta ttggcgcggt gcacaacaca gctaaagtcc agccaggtga
     4261 ttctgttgcc gtgtttggtc ttggcgcgat tggtctggca gtggttcagg gcgcgcgtca
     4321 ggcgaaagcg ggacggatta tcgctatcga taccaacccg aagaaattcg atctggcgcg
     4381 tcgcttcggt gctaccgact gcattaaccc gaatgactac gacaaaccga ttaaagatgt
     4441 cctgctggat atcaacaaat ggggtatcga ccataccttt gaatgcatcg gtaacgtcaa
     4501 cgtgatgcgt gcggcgctgg aaagtgcgca ccgcggctgg ggtcagtcgg tgatcatcgg
     4561 ggtggcaggt tctggtcagg aaatctccac ccgtccattc cagttggtca ctggtcgcgt
     4621 atggaaaggt tccgcgtttg gcggcgtgaa aggtcgttcc cagttaccgg gtatggttga
     4681 agatgcgatg aaaggtgata tcgatctgga accgtttgtc acgcatacca tgagccttga
     4741 tgaaattaat gacgccttcg acctgatgca tgaaggcaaa tccattcgaa ccgtaattcg
     4801 ttactgattt cccgcaggta taccccgtcc acttcagacg gggttcttaa tactctccct
     4861 gggcagccgt ccgggggatt aaccgtgaga taatgactga tggaactcat tgaaaaacat
     4921 gccagctttg gcggctggca aaatgtgtat cggcattatt cccaatcact gaaatgtgaa
     4981 atgaatgtcg gcgtctatct cccaccaaaa gccgcgaatg aaaaattgcc ggtgctgtac
     5041 tggctttcgg gcctgacctg caacgagcag aatttcatta ctaaatcggg gatgcagcgt
     5101 tacgcggctg aacacaacat tattgttgtt gcgccggaca ccagtccgcg aggcagtcat
     5161 gtcgcagatg ccgaccgtta cgatctcggg caaggtgccg ggttttacct gaacgcaacg
     5221 caagcgccgt ggaatgaaca ttacaaaatg tatgactata tccgcaacga gctgccggat
     5281 ttagtgatgc aacattttcc ggcaacgacc agaaagtcta tctctggtca ttctatgggc
     5341 gggctgggcg cgctggtgct ggcgttacgt aacccagatg aatatgtcag cgtctcggcg
     5401 ttttcgccca ttgtctcccc atcgcaagta ccgtggggac agcaagcctt tgccgcatat
     5461 cttggtgaaa ataaagatgc ctggttgaat tacgatccgg tgagtcttat ttcacaaggt
     5521 caacgcgttg cggaaatcat ggttgatcag gggttgagtg atgattttta cgcggaacag
     5581 ctgcggactc caaatcttga aaagatctgc caggagatga atatcaagac gttaatccgt
     5641 tatcacgagg gttacgacca cagctattat tttgtctcca gttttattgg cgagcatatt
     5701 gcctaccacg ccaataaact gaatatgcgt tgataatagt gcacgactgc cggatgcggc
     5761 gtgaacgcct tatccgccta catttcgccc gcaggccgta ggcatgataa gacgcgcata
     5821 gcgtcgcatc aggcatctgc gcacgactta tctatgttta ccacatcaaa tcatcaggca
     5881 ctttgaagtc ggcatatggg tcgtcttcat cctgctcttc ggcactgagc gcgctgtgca
     5941 acacaatact gctggcatca cgctgagcaa ttttatcggc aacgctggcg ggaataatgg
     6001 cgtattcacc ttcgctattg ttatcgacca acaagcgggc aatcgccaga cgaccattaa
     6061 tcaactgcgc ctgtgtgagc ttatcgacaa aaatcttctt aatcagattg ccgtcagtga
     6121 agttaaaacc aatatcacca ttggcaatgg tgattcggtt catttcaata agctgcttca
     6181 cctgggcttt atattctttc gccaacgccg cttgtttttg ctgttcgcta agctgtttat
     6241 cacgctcaag ctgtgccttt ttattttctt ctaccgccgc ccgagcttca cgcgcctgaa
     6301 cgcgcgattt tttcgccgtt ctctcgacct tcgccgcttt tttgctggtg actaatcctg
     6361 ctttgagcaa ctgctcttgt aaggtaagtt ttgccatctt tgtttccaaa cccgctgaat
     6421 aatttttagc gagtatacct gtaaacgccg tgggtgtatt gggggcaagt ccctttcgcc
     6481 cctgctaaat aaggcgcgtc aggcatcggc acacggctgc attcttgatc tccgactcat
     6541 caaaataaac accgccaacc cagcaacaag aatacccggt gcagaagcgg ccattacgcc
     6601 gaccgtgcca gtgcctaatg ccagcatttt cccggccagt aacggaccgc tcatagcccc
     6661 cagacgccct accgccacgg ctgttcccac acctgttgcg cggatctgcg aactgtaaaa
     6721 caacggtgcc agggcataca aaacgctttg cccacctgtc gcaaacaacc ccgcgacaaa
     6781 tcccgccagc aacataccgt taaatgacga caccgttcca agcgccagca gcgaagctaa
     6841 catgccgcta taaatcagta gcgacatggt tactggacgc agcttatcca tcaatgcgcc
     6901 caacattaac gtcccgcttg ccgcccccat ttgcagggca aacatcaccc ctgccgcctg
     6961 cgatggctgg aatccttgct ccaccaaaag tagcggtagc cagttgatca acatataaac
     7021 caccagcaga gtgaagaaat aacacaacca cagcagcagc gtcgcggttg ccgtttctgg
     7081 cgcaaataag gcacgcagtg gtggcgcagc ctgtttttcg ccagcgaaaa ccgctgactc
     7141 cggcagccag cgcattaata gcggcaccag aatcaacggc accacaccac ctacccaaaa
     7201 caccgtttgc catgctaagt ttgcccccgc gaaacccagt gtcgccgcca gcgccgcgcc
     7261 aatgggaaca ccgcaataca tcaggctcac tgccgtccca cgaaaacgtg gacccgcggc
     7321 ttcagacgtc agggcgataa gattcggcaa cgccgccccc agcccgacac cggtcatcag
     7381 ccgcgcaaag accagtgagg ggaaatccca ggcaatcgcc gttgccagtg agaacaaacc
     7441 aaacagcgca actgagccaa tcaaaatact cttacgacca taacggtctg ccagcatccc
     7501 gccaaccaac gcgccgggta gcaaaccgag tattccggcg ctaaatatcc agcccatttg
     7561 cattttatcg agtgcgaaag cctgggcgat gccacccgcc gcaatgccag ccgcctgaag
     7621 atccagccct tccatcagag cgaccaaaaa acaaagcccg atggtcagca tcaggcggga
     7681 tgaagatgat gaaggggtac gagtcgacat aagatgaacc tcgttgcgag tgcaggtgaa
     7741 tcgcgactga tacattgcca gccgcgtaat cagaactatt taatgactgc ctacgggcga
     7801 aatgtaggcc ggacaagacg tttatcgcat ccggcagtcg tgtgcagatg cctgatgcga
     7861 cgctaacgcg tcttatcagg cctacaaatt ctcacgccac ccgtaggccg gataaggtgt
     7921 ttacgccgca tccggcagtc gtgctgggat gggcatacaa aatgcacgtt ctcggcagtc
     7981 atcattattt gttgttgcgc agatccagcg ccacgtcaac gatcatatcc tcctggccgc
     8041 caaccatccg gcgtttgccc agctcaacga gaatatccac cgcacttaag ccataacgcg
     8101 ccgccgccgt ttcacagtga cgcaggaagc tcgagtaaac accagcgtat cccagcgcca
     8161 gcgtttcgcg atcgactcgt accggtcgat cctgcaacgg acgcaccagg tcgtcagcgg
     8221 catccattaa cgcatagaga tcggtcccat gctgccagcc cagtttatcc gcggcggcaa
     8281 taaatacttc cagcggcgcg ttacctgcgc ccgcgcccat tcccgcgagg ctggcgtcga
     8341 ttcggtcgca gccctcttcc accgccgcga tagagttcgc cacgccaaga ctcaggttat
     8401 ggtgagcgtg catgccagtt tgcgtttctg gtttcagctc tgctttcagg gcgcggaaac
     8461 ggtcacggat atcgctcatg ttcatcgcac cgccagaatc caccacataa atacaggtcg
     8521 caccgtagcc ttccatcagc tttgcctgct ttgcgagatt ctccggcgtg gtcatatggc
     8581 tcatcatcag aaaaccaacg gtgtccattc cgagctcgcg ggcatactga atatgctggg
     8641 cggaaacatc agcttcggta cagtgcgttg ccacacgaac cacccgcgcg ccagcctgcc
     8701 aggcattttt cagatcgtga atagtgccga ttcctggcag caacaacgtc gcgattttgg
     8761 cgtgcttcac cacatccgcc gctgcttcaa tccattcaag gtcgctatgt gcgccgaaac
     8821 catagttaaa gctggaaccc tgcaaaccgt cgccatgggc cacttcaatc gaatccacgc
     8881 gggcatcgtc cagtgctttg gcaatctggc gaacgttttc cagcgaatac tgatgacgaa
     8941 tggcgtgcat accgtcacgc aacgtgacgt ccgagatata aagtttttta ccgttcatgc
     9001 cgcttctcct gccttgcgcg ccagtgactg ggccattttt tccgctgtcg ccagcgcact
     9061 ggaagtcata atgtcgaggt tgcccgcata ggcaggcaga taatgcgctg cgccttcgac
     9121 ttccagccag accgctgttt tcagtccgga gaattgcccc acgcccggta aattgaccgg
     9181 tttatcctgc gggataactt caaactgcac gcgctgtttc aggcgataac ccggtacgta
     9241 agcctgcacc gcctcagcca tttcattgat tgaggcttcg atatcatctt gtgaagcttc
     9301 gtcgctcaat acatacaccg tgtcacgcat catcaacggt ggctctgctg ggttaagcac
     9361 aataatcgcc ttcccttttg ccgcgccgcc caccacttca atggctcggg aagtggtttc
     9421 cgtaaattca tcgatattgg cacgcgtgcc aggtccggca gatttactgg cgatagaagc
     9481 gataatttcg gcgtaatgaa cacgcgccac gcgtgaaact gccgccacca ttggaatggt
     9541 ggcctggccg ccgcaggtga ccatgttgac gttcagttga tcgacgttcg cctcgaggtt
     9601 aaccaccggc acgcagtaag ggccgatggc agcaggcgtc aggtcaatta agcgaatatc
     9661 cggtttcgct tcgcgtaaag cggcatcgtt tttcacatgc gcgcccgcgc tggtcgcatc
     9721 aaatacaatg tcgatatcag caaattcagg catgttcatc agcccgatca ccccttcatg
     9781 ggtagtggcg acgcccatac gtctggcacg cgccagaccg tcggactgag gatcaatgcc
     9841 gaccatcacc gccatctcca gatgctgacc gtgacgcaaa attttaatca tcagatcggt
     9901 gccaatgttg ccagaaccga taatggcgac tttacgctta ctcatgacag acttcctttt
     9961 ggggctgcgc ttgaaaatgt cgcagcaact gaacctatgc cttcaatatg ggcttcaaaa
    10021 cgatcgcccg cattcactgc caccatcgga cctaatgccc cggtaagaat gatatctccg
    10081 gcgcgcagcg gttcacccag gctggccatt ttgcgtgcca gccagacggc ggcattaagc
    10141 ggatgtccca ggcattcgct gccgcgcccg ctggaaacct cttcgttatt acgcgtcatc
    10201 ttcatggcgc agtttttcag gtctaacccc gccggacgtt gcgccggacc gccgatgaca
    10261 tacaccccgc aggaagcgtt atctgccacg gtatcgacaa actgaatcga ccagtcgcga
    10321 atgcggctcc ccaccacttc cagcgccgga agtacccatt caatggcgtt atacagttcg
    10381 tcgaaggtga tatcagttgc gggtaaatcg cggttcaaca ccagtgcgat ctccgcttca
    10441 atgcggggtt gcagaacacg ggaaaaagga atgatttcgt tatcgccata acacatgtcg
    10501 gcaaataacg tcccaaaatc cggttgatca acgcccagtt gttgttgcac tttcggatgc
    10561 gtcaggccca ctttacgccc taccacgcga cgcccctgca caacgtcata ttgcacattt
    10621 atgtgctgaa tggcgtaggc cgcttcagcg ttatcgatac caatcagatc gcgcagcggc
    10681 gcaatcgctt cgccctgctc tgcggcgcgg cgtaaatccg ccgccagttg ctcaagagta
    10741 tgcttcgtca tgaccattcc ttaagggcgt gcgaggaaat tcagcaccaa ttgattgaaa
    10801 gcgtcggcat gttcccactg cgcccagtga ccacagtcgc ggaagatatg cagttcagaa
    10861 ccggcaatgc cggacagcag acgcagaccc gcatccatcg gcacaaagcg gtcgttgcgc
    10921 ccccagacaa tcagggtttg cgctttgatt tccgccagac gtgggccaaa atccgggaac
    10981 tgtttcggat tagcttccag gctcttaacg aagttttcca ggtgatcgcg gcgcgacagc
    11041 atattattca ggcgcgcttc aaacagggcg tcggtcaaat cgctggtatc gaaaacgaag
    11101 atatccatca tcagcttcag gttttcgata gtcggctgac gataaagctg attcagtcgc
    11161 ttaatacctt cggttggcat cggcgtaaac aaactcatgc cgcccgtacc gccgcccatc
    11221 agcaccagtt tgccgacgcg ctccggccag ttaagggtga acgccacaga actatggccg
    11281 cccatcgagt tgcccagcag gtggattttg gcgatatcca gttgatccac cacgcttttc
    11341 aggattcgtg cattaagatc cgatcgtgaa ccactattaa cgatcgaatc gcttttgccc
    11401 caacccggac aatccagcag gattacccga tagcccgcct ctaccagcgg atcgatattg
    11461 cggctgaagt tcgcccagcc agtagcaccc gggccggaac catgcagcag gacaacggtt
    11521 tcgtcgcctt gtccgcagtc attaaaatgg atgcgcagcg ttttacccgc ttcttctaca
    11581 ttcagaaaac ggctggtggc ggcttcggtt tgtggctgat aactcatcat cttctcctgc
    11641 atattcagtt ctctgttctg gcgcttaacg agccaaatcc ggcaatccac tccgggattg
    11701 ggcggtaata acgcccttcg ctacgccagt tgccaaatgt agaaatagcg gcaaaagcgg
    11761 cgacccaggt tttgatttca tgtgtcgact ttccggcaat ggcggaaagc tcttcgttac
    11821 tgacggcatc cagttcctgt atgcgtccct gctccagcaa agtcatgaac tggttatccc
    11881 aaatcgggtt gagcggatgc agagttctct gatcctcaac aaacttctca gcggcgctaa
    11941 tcacccgttg ctgacgcaat tcgcgctcac tggcgggtaa atctttcccg ctccccaaca
    12001 gacggtcgcg catatgggca tcggctttcg ccagttcggg caccggcggc tgatgggaaa
    12061 gcccaccgga acccaggaac agcacgcgtt tattgagagt gctggtgaaa cgtccaatgg
    12121 cttcacccaa catgcgggta cgctggaaac cgggcagcgg cgtggcgaca ccgttgatga
    12181 acacaggcag aactggcacc ttatccagcc caccgagcag gaactccagc ggctgggcga
    12241 acccgtggtc cacctgcata cagtaagaaa cggcaagatc gatcccgctc ttcatgacag
    12301 catgcgcaca ggcctccgcc agctccacag gcacgggcag ctctcctgcc gcactgccga
    12361 aatcaccaat tgccgtcgct ccaacgccta aacagaacgg tggcatcacg tcatagaaaa
    12421 agccgttgta gtgatctggc gcaaacagca ccaccagttc aggggagaat gccgcaatac
    12481 gctcgcgggc gctggcaatc acgccattga cctcatcgag cacctcttgc gccgggtcga
    12541 cataccccac cagcggcgag tgggaaagac agtgaagata agcgtgcata tcaggctacc
    12601 ttttcgacag aaacgtcggc atcagggcgg gtcagcgtca tcaccgacgc cagtttattc
    12661 agggtcttgc ccaggatttg cggaatggcg gtggcggcaa caaagcgatc cgggcgcatc
    12721 accaccagcg aagcattgtg ttgcgcgaac cagctacgca gacgaccttg cgtatcgccc
    12781 acgcgtagca cgccgtcgtg gttatcctgt gcggtatgaa tttgcacttc cggcaccacc
    12841 tgaatgaagc gtgtgcccaa cgcgcgccac tgctggattt gctcgtcgct catcccccac
    12901 agtggattgc atccccagcc aattaccgcg aagttcgcgc cgatcgcgtt atcgagcagc
    12961 gtcacgtcgc cgttttccag cgtgactttc ggctgaataa acatcttgcc gaccggagag
    13021 tgcttcgctt cgccctcacg caccagcgca ccgccgtaat attgcggcat cggcttgaag
    13081 cgcatttcga ggaagtagcg ttttactggc ggcagataat tcaacagcca ggaaacgccg
    13141 tcacgtaacg taccctgcca gcgtttcggc ggagccagca cgttgcccgc cgtcacggac
    13201 aggtcaatca tcgctttggc gtgatcgcga cgttcttgtt gataggtatc gagcagcgca
    13261 tcgcgggctt tcccctggat aaccaacgcc agtttccagg cgaggttaaa ggcgtcgcgc
    13321 ataccactgt tatagccctg cccctgccac accggcatga tgtgcgcggc atcgcccgcc
    13381 agcagtacgc gatcaatacg gaaacgttgc gccagtcgcg cgttatgggt gtagacacgc
    13441 tggcgaatca attcaacatt gtccggatta ggcagcactt tgcttaacag cttgcgcata
    13501 ttttgcggct cacgcagctg ctcttcggtt tctcccggca tcaccataaa ttcaaagcga
    13561 cgtaccgcat gaggcagcgc ggcagaaaca tacgggcgca ccggatcgca acacaaatag
    13621 atatgcggcg tacttaacgg atcgttggcg atatctacca caatccactg atttggcgca
    13681 gttttacctt caaacggcac attcagagtg cgacggacaa aacttccccc accatcacag
    13741 gccaccagcc actgggcttt gactatttcc cgctgccctt ctgccgtttt caggtgcaag
    13801 gtcacttcgt cattttgctg gctgaaggcc tccagctcgc gggcaaacaa gcagcgcaca
    13861 ttcggaaaac gcgacaaccc ttccagcatc accgcatcga cctgtggctg aataaaggcg
    13921 ttacggcgcg gccagccaaa ttcatcggtc attggctgaa tatcagcaaa acagcggcct
    13981 tttggggtga gaaaacgcat cgcgtgccac ggtgtagtgt gcggcagaac attctcgacc
    14041 aggccgaccg actgcatggt gcgcagcgcc tcgtcatcaa taccaatcgc acgcgggtag
    14101 tcgatcaact tatcgagttt ctccaccacc agcacgtcaa tgcccatctg accgagatag
    14161 ttcgccatca tcagcccgac cggaccggca ccagcgatcg ccacctgaac gctatggtta
    14221 acagcaggct ggatgtcagg gtgttgtatt gccatttcag tacctcacga ctcggacaaa
    14281 atgccgttgc gcgcacaata cagcgcaact tattttgtta aaaacatgta aatgattttt
    14341 tattgtgcgc tcagtatagg aagggtattt tcggctacaa tcaaaacatg cccgaatgtg
    14401 caccaggtgc acaacgttgt tttaactata gaaatgtcaa ttaatatgca gaacaatgag
    14461 cagacggaat acaaaaccgt gcgcggctta acccgcggtc taatgttatt aaatatgtta
    14521 aataaacttg atggcggtgc cagcgtcggg ctgctggcgg aactcagcgg cctgcatcgc
    14581 accactgtgc ggcgactgct ggagacgctg caggaagagg gatatgtccg ccgtagcccc
    14641 tccgatgaca gttttcgtct gaccatcaaa gtgcggcaat taagcgaagg atttcgtgac
    14701 gaacagtgga tttctgcact ggcggccccg ctgctgggcg atctgttgcg cgaagtggta
    14761 tggccaacag atgtgtccac cctggatgtt gatgcaatgg tggtacgcga aaccacccac
    14821 cgtttcagcc gcttatcctt tcaccgggca atggtcgggc gacgtttacc gctcctgaaa
    14881 accgcctcgg gcctgacctg gctggccttt tgcccggaac aagaacgcaa ggaattaatc
    14941 gaaatgttag ccgcccgccc cggtgatgac tatcaactgg cacgagaacc gttaaagctg
    15001 caagccattc tggcgcgcgc gcgcaaagag ggttacggac agaactaccg cggctgggat
    15061 caggaggaga agatcgcctc tatcgccgta ccgctgcgca gtgaacaacg ggtgattggc
    15121 tgtctgaatc tggtgtatat ggcgagcgca atgaccattg aacaggcagc ggaaaagcat
    15181 cttccggcgc tacaacgggt agcaaaacag atcgaagaag gggttgaatc gcaggctatt
    15241 ctggtggccg gacggcgaag cggcgtgcat ttacgttgac accatcgaat ggcgcaaaac
    15301 ctttcgtggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa
    15361 accagtaacg ctatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttctcg
    15421 cgtggtgaac caggccagcc acgtttctgc gaaaacgcga gaaaaagtgg aagcggcgat
    15481 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
    15541 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
    15601 gattaaatct cgcgctgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
    15661 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
    15721 gatcattaac tatccgctgg atgaccagga tgccactgct gtggaagcag cctgcgctaa
    15781 tgttccggcg ttatttcttg atgtctctga ccagactccc atcaacagta ttattttctc
    15841 ccatgaagac ggtacgcggc tgggcgtgga gcatctggtc gcattgggtc accagcaaat
    15901 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg cgggctggca
    15961 taaatatctc acacgcaatc aaattcagcc gatagcggaa cgggagggcg actggagtgc
    16021 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
    16081 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
    16141 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcgtgtta
    16201 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
    16261 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
    16321 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
    16381 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    16441 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    16501 ttccggctcg tatgttgtgt gaaattgtga gcggataaca atttcacaca ggaaacagct
    16561 atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    16621 ggcgttacac aacttaatcg ccttgcggca catccccctt tcgccagctg gcgtaatagc
    16681 gaagaagccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
    16741 tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct
    16801 gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc
    16861 tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg
    16921 acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg
    16981 cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg tcgctgggtc
    17041 ggttacggtc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc
    17101 ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat
    17161 caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact
    17221 acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta
    17281 ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct
    17341 ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc
    17401 gatgagcgtg gtagttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa
    17461 ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac
    17521 ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat
    17581 ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat
    17641 catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg
    17701 aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac
    17761 acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc
    17821 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc
    17881 gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg
    17941 aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat
    18001 ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt
    18061 tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc
    18121 atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc
    18181 cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat
    18241 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat
    18301 gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc
    18361 cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa
    18421 gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc
    18481 agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat
    18541 ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg
    18601 attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc
    18661 gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag
    18721 tggcgtctgg cggaaaacct cagtgtgacg ctcccctccg cgtcccatat catcccgcaa
    18781 ctaaccacca gcgaaacgga tttttgcatc gagctgggta ataagcgttg gcaatttaac
    18841 cgtcagtcag gccttctttc acagatgtgg attggcgatg aaaaacaact gctgacgccg
    18901 ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc
    18961 cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa
    19021 gcagcgttgt tgcagtgctc ggcagataca cttgccgacg cggtgctgat tacaaccgct
    19081 cacgcgtggc agcatcaggg aaaaacctta tttatcagcc ggaaaaccta ccggattgat
    19141 ggtagtggtc aaatggcgat taccgttgat gttgaagtag cgagcgatac accgcatccg
    19201 gcacggattg gcctgacctg ccagctggcg caggtcgcag agcgggtaaa ctggctcgga
    19261 ttagggccgc aagaaaacta tcccgaccgc cttactgcgg cctgttttga ccgctgggat
    19321 ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc
    19381 gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc
    19441 agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa
    19501 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg
    19561 agcccgtcag tgtcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc
    19621 tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa
    19681 tccattatgt actatttaaa aaacacaaac ttttggatgt tcggtttatt ctttttcttt
    19741 tactttttta tcatgggagc ctacttcccg tttttcccga tttggctaca tgacatcaac
    19801 catatcagca aaagtgatac gggtattatt tttgccgcta tttctctgtt ctcgctatta
    19861 ttccaaccgc tgtttggtct gctttctgac aaactcgggc tgcgcaaata cctgctgtgg
    19921 attattaccg gcatgttagt gatgtttgcg ccgttcttta tttttatctt cgggccactg
    19981 ttacaataca acattttagt aggatcgatt gttggtggta tttatctagg cttttgtttt
    20041 aacgccggtg cgccagcagt agaggcattt attgagaaag tcagccgtcg cagtaatttc
    20101 gaatttggtc gcgcgcggat gtttggctgt gttggctggg cgctgtgtgc ctcgattgtc
    20161 ggcatcatgt tcaccgtcaa taatcagttt gttttctggc tgggctctgg ctgtgcactc
    20221 atcctcgccg ttttactctt tttcgccaaa acggatgcgc cctcttctgc cacggttgcc
    20281 aatgcggtag gtgccaacca ttcggcattt agccttaagc tggcgctgga actgttcaga
    20341 cagccaaaac tgtggttttt gtcactgtat gttattggcg tttcctgcac ctacgatgtt
    20401 tttgaccaac agtttgctaa tttctttact tcgttctttg ctaccggtga acagggtacg
    20461 cgggtatttg gctacgtaac gacaatgggc gaattactta acgcctcgat tatgttcttt
    20521 gcgccactga tcattaatcg catcggtggg aaaaatgccc tgctgctggc tggcactatt
    20581 atgtctgtac gtattattgg ctcatcgttc gccacctcag cgctggaagt agttattctg
    20641 aaaacgctgc atatgtttga agtaccgttc ctgctggtgg gctgctttaa atatattacc
    20701 agccagtttg aagtgcgttt ttcagcgacg atttatctgg tctgtttctg cttctttaag
    20761 caactggcga tgatttttat gtctgtactg gcgggcaata tgtatgaaag catcggtttc
    20821 cagggcgctt atctggtgct gggtctggtg gcgctgggct tcaccttaat ttccgtgttc
    20881 acgcttagcg gccccggccc gctttctcta ctgcgtcgtc aggtgaatga agtcgcttaa
    20941 gcaatcaatg tcggatgcgg cactgggcac atccgacaat tatttatagc tccatcatct
    21001 ggcgaaacaa gcgtcgcatc atacctcttt gacccacagc tgcggaaaac gtgctggtgc
    21061 aaaacgcagg gttatgatca tcagcccaac gacgcacagc gcatgaaacg cccagtccat
    21121 caggtaattg ccgctgatac tacgcagcac gccagaaaac cacggggcaa gcccggcgat
    21181 gataaaaccg atcccctgca taaacgccac cagcttgcca gcaatagccg gttgcgcaga
    21241 gtgatcgagc gccagcagca aacagagcgg aaacgcgccg cccagaccta acccacacac
    21301 catcgcccac gataccggca attgcagcgg caaccagata aagccgcaga accccaccag
    21361 ttgtaacacc agcgccagca ttaacagttt gcgccgatcc tgatggcgag ccatagcagg
    21421 catcagcaaa gctcctgcgg cttgcccaag cgtcatcaat gccagtaagg aaccgctgta
    21481 ctgcgcgctg gcaccaatct caatatagaa agcgggtaac caggcaatca ggctggcgta
    21541 accgccgtta atcagaccga agtaaacacc cagcgtccac gcgcggggag tgaataccac
    21601 gcgaaccgga gtggttgttg tcttgtggga agaggcgacc tcgcgggcgc tttgccacca
    21661 ccaggcaaag agcgcaacaa cggcaggcag cgcccaccag gcgagtgttt gataccaggt
    21721 ttcgctatgt tgaactaacc agggcgttat ggcggcacca agtccaccgc cgcccatcag
    21781 agccgcagac cacagcccca tcaccagtgg cgtgcgttgc tgaaaccgcc gtttaatcac
    21841 tgaaggcatc accacctgaa tgataccgat ccccacccca ccaagcagtg cgctgctaag
    21901 cagcagcaca ctttgggggt aaagctcacg catcaatgca ccgacggcaa tcagcaacag
    21961 actgatggcg acactgcgac gttcgctgac atgctgatga agccagcttc cggccagcgc
    22021 cagcccgccc atggtgacca ccggaagagc ggtcaacagg gcagccacgc taaagctcat
    22081 tccgctcgcc tggcgcaatt gcggtagcag tggcccgacg gaggtgagca gtggtcgcat
    22141 attaagacca atcagcacca gaaccagcag catcgtgaac gtcttttgat gattgcgcat
    22201 ggctgtcaga acggcttggt cggcagatat ttaccatcta aggtaatgac cgcacgttcg
    22261 ccaccttccg ggtccgccac tttcttaacg tcgagtttga agttaatcgc gctgataatg
    22321 ccatcgccaa acttctcatg aaccaacgct ttcagggttg tgccgtacac ctgcaacatt
    22381 tcatagaaac gatacatcgt tgggtcggtt ggaatacggt catcaatgca gccacgcagt
    22441 ggaatcatct gcaacagtag aatggcgtct tcgtcgagat ccagcttcgc cccgaccagg
    22501 cgggcggcgt cggcaggaag cgcttgctgc cccagcaaag ccgcggttac gaaggcttct
    22561 gccagaccgg taccgtcggc aatctcggca aatgagagat cttttttagc tttgctgagc
    22621 aaaatggcat cggcaagatc aagacgaata ttgcggttaa tttgtgactg aatcatggtg
    22681 gatctcctga tggtttaaaa ataaggttac gctgcggtcg gttggcgtag cggtatggca
    22741 caaacgcgag gattagcggc cagtggcaca aactggcggg ttgcgccgtc aaaagctgcg
    22801 atgctgccgc tttcaatgtc gtagacccag ccgtgcaggg cgatccgccc ctcttcgagc
    22861 gccaggcgca ccgatggatg agtttgcaaa ttagccaact gagcaatgac gttttcacgt
    22921 accatcgccg cagcttttga cggtaaatcg gaatgcgggc gcgcctcatt aacgacgcgg
    22981 gctgaatcgg cataacgtag ccagtgggag acggcaggca tatggtccat gcactgacag
    23041 ctggcaatgg cggtcatcgc gccacagttg gaatgaccac aaatcacaat gtcagatacc
    23101 cgaagcgcag cgacggcata ctccaccgaa gcagaaacgc caccgggttc cggcccgtag
    23161 gaagggacga tattgcccgc gttgcgaata acgaacagat cgccaggctc acgttgcgtc
    23221 accagctcag ggaccagacg gctgtcggag caggagataa aaagtgtgcg cgggctttgc
    23281 tgtgtcgcca gctgtttaaa caaggcttcc cgcttcggaa atgcctcgcg ctggaattta
    23341 aggaatccat caataatctc tttcaccgtt aacctctgtc tgtctctgga atgagaggca
    23401 gactactcgc cgattgtcat aaggtaaaag tctcatttat gatgagttct attggattta
    23461 cttataggtt ggcgaatgct ctctcgacat atcaattatt ttcttgccgt ggctgaacat
    23521 ggcagcttca cccgtgccgc cagtgcgttg cacgtctctc agcctgcgct ttcccagcag
    23581 attcgccagt tagaggagag tttaggcgtg ccgctgtttg accgtagcgg gcgaacgatt
    23641 cgtctcaccg atgcaggaga agtctggcga cagtacgcca gccgggcgtt acaggaactg
    23701 ggagcgggta aacgggcgat tcatgatgtt gccgatctga cgcgaggatc gctgcgtatc
    23761 gccgtcaccc ccacctttac aagctacttt atcggccctt taatggcgga tttctatgcg
    23821 cgctaccccg gcatcacgct ccagctacag gaaatgtcgc aggagaaaat cgaagatctg
    23881 ctttgtcgcg acgagctgga cgttgggatt gccttcgcgc ctgtgcattc gccggagctg
    23941 gaggcaattc ctttactgac agaaagttta gcgttagtcg tggcgcaaca tcatccgctg
    24001 gctgcctgtg aacaggtggc gttgagtcgc ttgcatgatg aaaaactggt cctgctcagc
    24061 gcggaatttg ccaccagaga gcaaattgac cactactgcg agaaagcggg gctacatcca
    24121 caggtggtca ttgaggcgaa ctcaattagc gcggttctgg agctgattcg ccgcacttcc
    24181 ctttccacat tgttaccagc agcgattgcc acacaacatg acgggcttaa agcgatttct
    24241 cttgccccgc cactactgga gagaacggcg gttttgttgc ggcggaaaaa tagctggcag
    24301 acagccgccg cgaaggcatt tttgcacatg gcgttggagg aatgtgcgga tgttggcgag
    24361 aatgaatcac ggtagccact gacacaggtc agtggctctt caacacagag gaaattaccg
    24421 tttgtaatcg atggcttctg gctgctccag atatacggtg gtttgtgccg gttgtgtact
    24481 ggcaatcacc ttgccgccac gtaccgaata acgtaccgga acctgacggc gcagcgcatc
    24541 aaatccattt tcagccggca ggataatcag gtttgcgctg tttccggcgg caatgccgta
    24601 atcctgcaaa ttcaatgttc tggcactgtg gtgggtgatt aaattcaggc catcgttaat
    24661 ctgcccgtag cccatcaact ggcaaacatg cagccccata tgcagcactt gcagcatatt
    24721 cgccgttccc agcggatacc acggatcgaa gacatcatcg tgaccaaagc agacgttaat
    24781 gccggactcc agcatctctt taacgcgcgt gatgccgcga cgttttggat acgtatcgaa
    24841 acgtccttgc agatgaatat tgaccagcgg gttggcgaca aagttaatac cggacatttt
    24901 cagcaagcgg aacaggcgtg aggtatacgc cccgttatag gagtgcattg ccgtggtgtg
    24961 gctggcggtg actcgcgcgc ccatgccttc acggtgcgcc agggcagcaa cggtttcgac
    25021 aaagcgcgac tgctcgtcat cgatctcatc acagtgaacg tcgatgagac ggtcgtattt
    25081 ttgcgccagg gcgaaggttt tatgcagcga ctccacgccg tattcacggg taaattcaaa
    25141 atgcggaatc gcccccacta catctgcccc taagcgtaac gcctcttcca gcaacgcttc
    25201 accgttggga tacgacaaaa tcccttcctg agggaaggcg acaatttgca gatcaatcca
    25261 cggcgcgact tcctgcttca cttccagcat tgctttcagc gcagttagcg ttgcatccga
    25321 aacatcgaca tgggtacgca catgctgaat gccgttggca atctgccatt tcagcgtttg
    25381 ccaggcgcgt tgtttcacat cgtcatgggt taataacgct ttgcgctcgg cccagcgttc
    25441 aatgccttca aacagcgtgc cggactgatt ccagttcggt tgtccggcgg tttgcgtggt
    25501 gtccaggtga atatgcggct ccacaaacgg cggtataact aaaccttgtt cggcatccag
    25561 gctgttttca gttatgggca tcacgccgga ttgcgcatca atggcgctga tttttccgtc
    25621 ctgcagatga atctgccaca gcccctcttt gcctggtaac cgggcgttaa taattgtttg
    25681 taaagcgtta ttcgacactg ttaacctcca catgcgtcat tgctgctgtc gttttacgat
    25741 tcaaaatcgg gttaaggatc agatagctca gcgcgccacc taataccgcg ttgaccggaa
    25801 caattcccgg taaccagtgg cctgcggcaa tccccagcgc gaccgccaga atcgccaccc
    25861 aattgacact catcatacgc gtggtcgcaa agtgctcata gcggcgacgg ttcatcagat
    25921 agtcggcgat gatcacgccc cctaccggag gaatagctgc cgaaaggaag gtcaaccagc
    25981 cgacaaaatt gttatacagc cataatgcgc agaccgtacc gataataccg ttgattaccg
    26041 aaagggtttt gctcgacata ccggtaatgt tggcgaaacc taaacccgac gcatagagtg
    26101 cgttatcgtt ggtggtccag atattcagcc ccagcaccac aatcgcgggc agcagcagtc
    26161 cctgagcaat catcacatca gagatatctg ccatccccag tgccgcagcg cccgctgcac
    26221 cgaaaataaa catcaacgag ttgccgagga aaaaggccac catcgccacc agcaccgcca
    26281 gtttggcatt gcgaccaaac cggacaaagt cagcggtgag cgtacccgca ctgataaatg
    26341 accccacaac cagcgccagc gcgacattga aatctaacgg ttgtgcggga acgaccgctt
    26401 ttaaggcgtc caggccgccc atgccgttaa cggccagcca cacggaataa ccgccaaggc
    26461 aggcaatagc cggaaccgca atcaccgaaa gaaccgtcag cgccgaaatg ccaaagaaga
    26521 cggtgacggt catcagtaaa ccggaaacgg caatcagcaa attaatatcc agcccggttg
    26581 ccttgcccac cggaatagca aacatcgcca cgccaacgcc aaaccagcca acctgagttc
    26641 cgcccagtag cagtgaaggc agccatgagc ctttaacacc aaacgagaag cgagcaagaa
    26701 gatgagtggt caggccggtt tttgcgccaa tgtaaccgag aaatgaagtg taaataccga
    26761 ggagaagatt accgatgaga actgcgagga agaaatcatg atagctaaga ccggttccga
    26821 gagtgccgcc ggtccacata ctggcggaaa agaaggttaa tcccagcatg acgaacgtca
    26881 atgccaatac ccctttccgc gccgactgcg ggactggccc ctggctaaag ttgttatctt
    26941 gcgacacgaa attccccctt tttgctgttt gaaacccaaa aaatccgccg cattctattc
    27001 atctgaagat aaaaagcaat cgttttcgtg gggaaatata ttttttatat gaatgagtgc
    27061 aaaaaggtgg gggaaaaatt atcggtgtaa aaacagtggg taggcataat aagacgcggt
    27121 aagcgtcgca tcaggcatcg gcgcatggtg tcggatgcgc cacgcgacgc atccgacaat
    27181 accttactct tccatcgcct ggcggatctg atccaacgac gctggatcat caatggtcgt
    27241 cagatctcca ggatcgcgtc cttcgcaaat cgcctggatc gtgcggcgca gcatttttcc
    27301 ggatcgcgtt tttggcaatt gcgagacaaa ccagacgtgc gccgggcggc caaagttgcc
    27361 aatctggctg tccaccagcg ccataatcgc cttctcttgc gaatgcgcca catcacgatc
    27421 ttccagactg tcgctctctt tcggaatgac aaacgctacc gccacctgcc ctttcagcgc
    27481 atctttcacc ccaaccaccg ccacttcggc aacgcccgga tgactggaaa tactctcttc
    27541 aatctcgcgc gtgcccagcc gatgcccggc aacgttaatc acatcgtcag tgcgcccgag
    27601 aataaagtga taaccgtcag catcgcggat gccccagtca aaggtggcgt acaccggacg
    27661 ggaaaacagc gaccagtacg tcttcacaaa gcggtcgtca tcgccccaga tggtctgaat
    27721 acaccccggc ggcagcggcc cttccaccac cagcatccct ttctcgttga cgccacacgg
    27781 ttcgccggtg acttcattaa gcaactgcac gttatagcca tacatcggca cgccggggct
    27841 tcccagacgc gtcggcctgt cgtccagacc gcgagcaatc gccataatcg gccagccgga
    27901 ttcggtctgc cagtagttgt cgatgaccgg cacatccagc gtattgctca cccaactggc
    27961 ggtcggctcg tccagcggtt ctccagccag atagagcact tccagcgacg agagatcgtg
    28021 tttgcgaatt tcagcggtag ggaatttttt cagcacgcga atggcggtcg gcgctgagaa
    28081 catccggctc acctgatatt tctcgacgat tttccaccac acgccgcagt ccggccaggt
    28141 cggtaatcct tcgtaaacga tagtcgccat ccccgccagc agcggcgcgt agacgatata
    28201 cgaatgcccc accacccagc cgatatccga agcacaaaag aacacaccgc ccgctttgcc
    28261 gccaaaaatg gtgtccatcg aggtcgccag cgccaccgca tatccgccga cgtcacgctg
    28321 cacgccttta ggtttgccgg tcgtgccgga ggtgtagaga atgcaggagg tttcgttgga
    28381 ttccagccac gccaccggta cccgcgcgcc gatgtgttga tggcgcaacg acgcgaaatc
    28441 gacatcccgc ccgctgacgc gtgccatttt cgccagcccg cgatccacca gcaaaacatg
    28501 gcgtggctgg tgctgcgcct gacttatcgc atcgtcgagc aattttttat agggaatgat
    28561 tttgccaccg cgcgctccgg catcagccga gacaatcagc accggtttag catcatcaat
    28621 tcgcgccgcc acgctgtgcg aggcaaatcc cccaaacacc accgaatgaa tagcgccaat
    28681 gcgcgcgcag gccagcagag taatatgcgc ttcggcaatc atcggcatat acaccagcac
    28741 ccgatcgcca cgctgcacgc ccagcgagcg cagcattgac gccaccgcgt ttacttcgtc
    28801 atgaagctga cgaaaggtaa aggtgcgctc ttcttctgtt tccgaagaga cggcaatcag
    28861 cgccagcgcc tctggttgtt tctccagcca gcggtcgata gcgttgtggc acaagttggt
    28921 tcggccttca caaaaccaac gggcaaacgg cggattgctg tggtcgagcg tttgcgtaaa
    28981 gggcgtctgc cagtcaatac gccgggcctg ctcggcccag aactgctccg gttcgttaat
    29041 cgaacgctga taaaattcgc taaaagacat aatgctctcc tgttgaactt acgccttacc
    29101 gccgtttact taaatgacgt acaggtcgag atactcattg accggcatct gttccaggcg
    29161 agttctgtcg agagaaacct ccagaatgcg ctgctgttgg cgagtcggga actggcgcgc
    29221 gagattgatt ttgaatttat cgaccagttt cggaatacca tcctgacggc ggcgagcatg
    29281 accaatgggg tactccacca ccacttcttc aaatcgtgtg ccgtcggtga actcaagggt
    29341 aatggcattg gcgatggcgc gtttttccgg gtcgtggtag tcagcggtaa atgccggatc
    29401 ttcaaagcaa ttgatcttct cgcgcagggc gtcgatgcgt ttatcttgcg ccacgttgtc
    29461 ctcgtaatct gccgccgtta agcgcccgaa cagcagcggg atcgccacca tgtactgaat
    29521 gcagtgatcg cggtctgccg ggttattgag cggtcctttt ttgtcgatga tgcgaataca
    29581 ggcttcgtgg gtgcgaatgg tcactttttc gatatccgcc gccgttttgc ctgctgcctg
    29641 catctgttca tagagcgtca tcgctgcttc aactgccgtc tgggagtgaa actccgccgg
    29701 gaaggagatt ttgaacagca cattttccat aacgtaggaa ccgtacgggc gctggaagcg
    29761 gaacgattca cctttaaagg agacgtcgta gaagccccac accggcgcag tcagggctga
    29821 cgggtaaccc atttcgcccg ttttcgccat cagtgccaga cgtaccgcgc gggaagtggc
    29881 atcgcccgcc gcccaggatt tacgcgtgcc ggtgttcggc gcatggcgat aggtgcgcag
    29941 cgactgaccg tccacccacg ccagcgaaac ggcgttgaga atttcctcgc gggtcaggcc
    30001 gagcatttcg gcgaccacgg cggtggaagc cactttcact aacagaacgt ggtcgaggcc
    30061 gacgcggtta aaggagtttt ccagcgcgat gcagccctga atttcatggg ctttgatcat
    30121 tgcggtcagc acctgtttca tggtcaacgg cgctttgccg ctggcgaccg cgttgcgcga
    30181 aagccagtcc gccgttgcca gaatgccgcc gaggttgtcg gaaggatggc cccactccgc
    30241 cgccagccag gtatcgttga aatcgagcca gcggatcatc gcgccgatgt taaatgccgc
    30301 ctggacgggg tcgagctgga actgagttcc ggggacgcgc acgccgttgg gtacgacggt
    30361 gccgggaaca attggcccca gcagtttttt acaggccggg tattcgagag cttccagacc
    30421 gcagccgagc gtgtcgagca ggcagtaatg tgcggtgtcg taggccactt tagagctgat
    30481 ttcgtagttc atgacgtaat cgacgatatc aacgatttca cgatcaaatt ccgggcggat
    30541 gttgttgatt tgagctgaca ttgggtacgt ttccttattg ttattcgtag aggtttactg
    30601 gcgcttatcc agcgcgacaa actggcggtc ttccggtcca acataattgg cggaaggacg
    30661 gataattttg ttgtcctgac gttgttcaat gatgtgcgcc gcccagccgg tgacacgggc
    30721 gatgacaaac agtggtgtga acatctcggt gggaacgccc atcatgttgt aggaaacagc
    30781 agagaaccag tcgagattgg ggaacatctt tttgctctcc cacatcaccg tttccaggcg
    30841 atcggcgatg ttgtacatct tcaacgagcc gccttcctgc gagagctgct tcgccacacg
    30901 tttgatcacc tggtggcgcg ggtcggcgat ggtataaacc ggatgaccaa aaccaatgac
    30961 cacttctttg ttttccaccc gcttgcggat atcggcttcg gcttcgtccg gcgtttcgta
    31021 gcgttgctgg atctccagcg acacttcatt cgctccgccg tgtttcggcc cgcgcagtgc
    31081 gccaatcgcg ccaataatcg cggaatacat atcagagcca gtgcccgcaa tcacccgact
    31141 ggtaaaggtg gaggcgttaa actcgtgttc ggcgtacagc actagcgaaa tatgcatcgc
    31201 cttttcccag ctttgcgatg gcttttcgcc gtgcagcaga tgcaggaagt gaccaccgat
    31261 ggagtcgtca tcggtttccg gctggatgcg ttcgccgttg tgactgtagt gataccaata
    31321 aaggagaatc gagctaagtg atgccagcag tttgtcggca atatcccgcg cgccagagac
    31381 agtgtgcccc tctttttctg gcagtgtgca gccgagcgcg gaaacgccgg tgcgcatgac
    31441 atccatcggg tgcgacgccg ccggtaaggc ttccagcacg gtacgcacat tagctggtaa
    31501 accacgcagg gctttcagtt tcgttttgta agcggcgagt tcgtcacggg tcggcagttt
    31561 gccgtggatc agcaggtgcg ccacttcttc aaattcgcaa tgttccgcca gatcaagaat
    31621 atcgtagcca cggtaatgca ggtcattgcc acttttaccc acggtgcaga gcgccgtatt
    31681 gcccgccgga acgccagaaa gtgctaccga ttttttcggt ttaatgacat gggtactgtt
    31741 ttgcaggatc gttgtgtcgc tcatattgtc ctcgtcattt gtagggtcga gatatttttc
    31801 gttgtgtttt atttcacctg gctacgggca aacaggtcgt cgagcttctc ttcgtactgg
    31861 tagtagttga tgctttcgta cagctcgttg cgggtttgca tggtatcgat gacgcttttc
    31921 tgcgtgcctt cctgacgcag gacgttgtag acatgttcag cggcgcggtt catggcgcgg
    31981 aacgctgaaa gcgggtacag cgccattgcg acatgggcgc tgcgtaattc gtcggtggta
    32041 aacagcggcg tggcaccaaa ttcggtaatg ttggcgagga ttggcacctg caccgcatcg
    32101 gcaaactggc ggtacatggc gagttcggta atcgcctccg ggaacaacat ctcggcaccc
    32161 gcttcaacat aggcctgtgc acgctcgatc gccgcatcca gcccctctac cgccagcgca
    32221 tcggtgcgcg ccatgatcac aaaatcagga tcggttttcg catccaccgc cgcgcggatc
    32281 cgatccacca tctcctcttt cgagacgatc gctttgttcg ggcgatgacc gcagcgtttc
    32341 gcaccaacct gatcttcaat atgcaatcct gccgcaccgg ctttaatcat cgatttcacc
    32401 gtacgcgcca cgttaaaggc cgaagaacca aaaccgatat ccgcatccac cagcagcggc
    32461 agcgaacaaa cgtcggtgat acggcgaatg tcggtcagca catcatcaag ggtagaaata
    32521 ccgagatcgg gcagccccag cgaacctgcc gccacgccac cgccggagag ataaattgcc
    32581 tgatatccgg cacgctgcgc caacagcgca tgattagcgt tgatggtgcc aacaatctgc
    32641 aatggatttt ctttagacag tgcagcgcga aacgctttac ctggagagtg tagagacata
    32701 gcccatcctt tgttatcaac ttgttacttg cgttgataaa gacaaagcaa ggggtgtgcc
    32761 acaattgcct taataaaatt aaatgtttaa tttcaatttg ttagagataa agtcatgaac
    32821 taatccgcaa atatgcgttt cagttaacgt ttcaggcaat gtttcaccgc gtttcattgc
    32881 aacaattatg aaacaagact aaacccaata ttcggtttct taactttgcg gtgcgctatg
    32941 gcacatccac cacggcttaa tgacgacaaa ccggttatct ggacggtatc tgtaacgcgc
    33001 ctgttcgagc tgtttcgcga tatcagcctc gagtttgatc acctggcgaa cattacccct
    33061 attcagcttg gctttgaaaa agcagtgacc tacatccgca agaaactggc aaacgaacgc
    33121 tgtgacgcca tcatcgcggc tggctctaac ggcgcgtacc tgaaaagccg cctgtcagtg
    33181 ccagttattt tgattaaacc gagcggctac gatgtgttac aggcactggc aaaagccgga
    33241 aaactcacct cttctatcgg cgttgtcacc tatcaggaaa ccattccagc gctggtggcg
    33301 tttcaaaaaa cctttaatct gcgcctcgat caacgtagct acattaccga agaagacgcc
    33361 cgcgggcaga ttaacgagct aaaagctaac ggcactgaag cggtggtcgg cgcggggctg
    33421 attaccgatc tggcagaaga agccggaatg accggaattt ttatctattc cgccgccacc
    33481 gtgcgccagg cgttcagcga tgcgctggat atgacgcgca tgtcgttacg ccataacact
    33541 cacgatgcca cccgcaacgc cctgagaact cgttacgtgc tgggcgatat gctcggtcaa
    33601 tcaccacaga tggaacaagt acggcagact attttgctgt atgcccgctc cagtgcagcg
    33661 gtgttgattg agggggaaac ggggacgggc aaagagctgg cggcccaggc gattcatcgg
    33721 gaatattttg cccgccacga tgcgcgacag ggcaaaaagt cgcatccgtt tgttgccgtc
    33781 aactgcgggg cgatagccga atcgctgctg gaagcggagc tgtttggcta tgaggaaggg
    33841 gcgtttaccg gctcgcgacg cggaggtcgc gccgggctgt tcgaaattgc ccacggcggt
    33901 acgctgtttc tggatgagat tggcgaaatg ccgctacctt tgcagactcg cctgttacgg
    33961 gtgctggaag aaaaagaggt cacccgcgtc ggcgggcatc agcctgttcc ggtagatgta
    34021 cgggtcatta gcgccactca ctgcaagctg gaagaagata tgcagcaagg acgttttcgc
    34081 cgtgatctgt tttatcggct gagtattttg cgtctgcaat tgccaccact gcgcgagcgg
    34141 gtggcggata ttcttccgct ggcggaaagc tttttgaaag tgtctctggc ggcgctctcc
    34201 gccccgtttt ctgccgcatt acgccaggga ttacaggcaa gcgaaaccgt gctggtgcac
    34261 tacgactggc caggtaatat tcgtgaactg cgcaatatga tggagcgact ggcgctattt
    34321 ttaagtgtgg aaccgacgcc ggatttaacg ccgcaatttt tgcagctgct actgccggaa
    34381 ctggcgcgcg agtcggcgaa aactcccgct ccacgcttac tgacaccaca acaggcactg
    34441 gagaaattta atggcgataa aacagcagcg gcgaattatt taggcatcag ccggacgacg
    34501 ttctggcggc ggctgaaaag ctgaactgcc gaatgcagcg cgaacgcttt atccggcata
    34561 aatttcaata gatgatcacc gataaactta cgcatcgggc atgtttacat agggttcaga
    34621 attacttctt cttataaata tttgccgtgc cgtggatctt attgtcagtt tgaccggagg
    34681 tcagtaccaa cacatcagcc cctttttcat ccgctttttt gatcaaatcc tctttcgcat
    34741 ctgcggtcga catttcatta ctggttgaaa tatcacctat tttttcatac tgcgattcaa
    34801 ctttttcaaa ttccgctttg gtcatcaatt cagcggcata gacattggta acggctaacg
    34861 ctaacgcacc gactaacatt ttagagatta ttttcatatt acggctccag agttgttatc
    34921 acactatcac ccacccacat tattgggttg attaataatt agttcagtca gggcataaac
    34981 gccagtaacc ggaaataaga cactttgtga tctctctcgc acccgattat ttttggtaga
    35041 aatatgatga agttacttca cttatttatg gatgaaatca ctatggatcc tttgcacgcc
    35101 gtttacctga ccgtaggact gttcgtgatt acttttttta atccgggagc caatctcttt
    35161 gtggtagtac aaaccagcct ggcttccggt cgacgcgcag gggtgctgac cggactgggc
    35221 gtggcgctgg gcgatgcatt ttattccggg ttgggtttgt ttggtcttgc aacgctaatt
    35281 acgcagtgtg aggagatttt ttcgcttatc agaatcgtcg gcggcgctta tctcttatgg
    35341 tttgcgtggt gcagcatgcg ccgccagtca acaccgcaaa tgagcacact acaacaaccg
    35401 attagcgcct cctggtatgt cttttttcgc cgcggattaa ttaccgatct ctctaacccg
    35461 caaaccgttt tattttttat cagtattttc tcagtaacat taaatgccga aacaccaaca
    35521 tgggcacgtt taatggcctg ggcggggatt gtgctcgcat cagttatctg gcgagtcttt
    35581 cttagtcagg cgttttcttt gcccgctgtg cgtcgtgctt atgggcgtat gcaacgcgtt
    35641 gccagtcggg ttattggtgc aattattggt gtattcgcgc tacgcctgat ttacgaaggg
    35701 gtgacgcagc ggtgagcaga taatttactc tctacgtaat caacctgatt tgccgaatcc
    35761 cggccatcgg cgatgatagc ccccgtaatg cgtggtcctt atttccgata agtttacttg
    35821 agtcttgagc ttcccatttc agcccactct cccctgtgtg ggcttttttc attggtctgc
    35881 cccgtcctga atagccaaac aatggcaatg ttccactttg cagggatgat aataagggga
    35941 tctgattcag aagagttttg aaaagttgga ccgccatcga ggcctcgaac cccgtaccat
    36001 acaacaatcc tggttgtcgt ctgctcttcc tgatgagcta atggcggttt ttgatatgag
    36061 ccttacagca tattttctgt caggcttgag tgcgatgcgt aagatacaga aaagacccca
    36121 cacaaacatg atggaattat gatttttttc atatgaatgt caacccacac attcacaaaa
    36181 catctgcatg agatcatttt ttatacaccg catagctgcg aaaccactgc tggtgctttt
    36241 ttaagcgaag cgtcgccgat cacaacaaaa tatcggcgaa aaaggcggtg gaatgccatt
    36301 tggcatacgg atatacagat gaaatgatta cctgctttgc gattcactct ctggtggaat
    36361 aacaacttgc aggtggaacc ctttctgaac taaagagtgt agcggatttt aacgtgctta
    36421 tttacaggat aatatctgtc acctaattcc tgcactctat tagatatcca ttcatttaat
    36481 cgtgcgtcat ctctgtatgt ttttacgtcc agaacagaga gcaataacat caccagtgat
    36541 tcaggaaaaa cagggtagat attttttaag tgtaataata atccatgcgc aagcgcctgc
    36601 ttaatgggcg cgtctgtctc cggaatagac gacatttcgc gaagccattt ttccacatgc
    36661 atacactctt tccctttagg gaaaattctc agcaaaggca tatggtgttc aagaagaata
    36721 catttgcaat ctgttcgcac tgaatcgata aactgcacta atttcttaag cccggtaaag
    36781 cgtccagcct gcacatttgc tttaatatca agaaacgcat catcctggtc atatacccaa
    36841 actgtccctt tttgattttc aagtatgcga aaaacaaact taaccgggtg atcagggtaa
    36901 tccatccagt cataaaatcc agggaaatca ttaaactgca catttatggt gagtgcgtta
    36961 tcgtctaacc actcacagtc tcgtacggca gccatgagat gaatgagttt gtcgtcatcg
    37021 actaaatcat cacgacaatc ataaggataa tcataatatt gtttaatcaa aaccttgaat
    37081 atatcgacac aggaaaccaa ggagcttttg atatcctctg gtaactgatt gtttttaacc
    37141 gtattaagta tgctttgttg cgtatatggc atcaaattat tgtgcggagc ttttaatgat
    37201 atcatcgcga tctctcttaa ataaaaataa acgtcgcaga taataaagct ctcattaaac
    37261 caggcactat cagaaatcgc tcataactaa cgtgagaagt attgtgtact ggagggagtt
    37321 aatttaacaa tttgaattta cgttataggt tttactgcta ctttgatatt atttcgctct
    37381 ccttaactaa aaagttaagt ggagagcgaa gttaatatgt taaaccacag cgtatttatt
    37441 aatcttttca gtctgttagt gtgcgattat cgataacaaa acgatatttc acatcaccac
    37501 gcagcattcg ctcataggct tcattaattt gatcggcccg aatcatctct atatcagcca
    37561 cgatgccatg ttcggcgcaa aaatcgagca tctcctgagt ttctggaatg ccgccaatca
    37621 tagaaccggc tatcgcacgg cgtttcatga tcaggttgaa aacttccggc gatttatgcg
    37681 gtgtcgcagg cgcaccaacc agcgtcatgg tgccatcacg cttcagcaag gtggtaaaat
    37741 cgtcgagatt atgtggcgca gctactgtat tcaaaatgaa atcgaaactc ttcacatgag
    37801 ccgccatctc atcggcattg cgtgagttaa caacttcatc ggcccccagg gcttttgccg
    37861 cttcgcgttt tgcctcagaa gtggtaaatg ccaccacatg cgcccccatc gcgtgggcca
    37921 gcttgatccc catatgtccc agaccgccga tgccgaccac gcccactttt ttacccggcc
    37981 cggcctgcca gtgacgtagc ggcgaatacg tggtgatccc tgcacacaac aaaggagcca
    38041 ccgccgccag ctgctcttgc gggtgacgaa tacgcagaac atatcgctca tgaacgacga
    38101 tctgttgtga gtagccgccc agagtatggc ccggttcgtc cggcgtcggc gagttatagg
    38161 tgccggtcat gtgatcacag tagttttcca gcccgtcttc acactcttcg caatgtttac
    38221 aactgtcgac aatgcagccg acaccgacca gatcgcccgg cgcatgtttt tctacctgat
    38281 caccaacggc taccacacgc cccacaattt catgacccgg tacgcagggg taaaccgtcc
    38341 ccgcccactc ggaacggacc tggtggagat cggaatggca gacgccacag taagcgattt
    38401 cgattttgac atcattcggt cccggttcac gccgtgtgat atccatcggt tcaagtggtt
    38461 gtttagcgga atatgcacca acagctttga tcttcattgt gtttactcct gattagctat
    38521 gtgtatttgg cgatgcctga tatgtcatcg cggcaaaacg cgtccattga atatagccaa
    38581 tatttaaacc acccgatcca gccccaacat gccgcgaacc tgaatgaaac agagcagcaa
    38641 cgttccccat aaggccatcg tcaggtaggg gcttataccg agaatattga agccgctttc
    38701 aataatttgc agaagaaaca gcgccagcac cataccaatt atccgcccgc tgccgccgtc
    38761 cggattaacc ccaccgagta ctgccgcgag gattgacacc agcaagtacg attcgccata
    38821 agaggcctta gccgagttga gtttcgacat catcaaaaat gccgctaccg cacaaagcaa
    38881 ggcggaaatg acataaaccc aaatcagcac ccgacgagtg ttaatgccgg agtaaagtgt
    38941 tgctcgttcg ttagacccaa tcaggtaaat ggctttaccg agcggcgttt tctccagcaa
    39001 tatccatagc cccagtgcca ctgcggcaaa caaccacatc ggtagcggaa taccgaacca
    39061 ttgcgcatga ttcaaccaca gcacccatga gggataattg gcaatggcgc taccacctgt
    39121 caccagaata tttatccctt tgagcaacgt catcatccca agcgttgcaa gaattggcga
    39181 cactcgaata ccagcaatca atacgccgtt acataaaccg attatcaccg ccgcgccagc
    39241 gcccgccagt aacgtagcga caaccgtagc aatacccggt ggatactgag tcgccaccca
    39301 cgccatcacc agtgaacagg cgttagcagt ggctataatt gacaagttaa tgccaccgca
    39361 aagcatggtc accgccatag ccaacgccag aatgcccagc acgggcattt gcgaagcgac
    39421 ggactggaaa ttacttatcg accagaaaat ctccggcata gtaatgctga acgcggcaac
    39481 agttagcacc agcaggccga tcaagtaaaa ctcaacgtta ttacgccacg attttttcat
    39541 agcagactcc ggttctgatg ctgactccac gccgtggcac taatgctggc aacgatgatg
    39601 atgccggtga tcaatgtttg ccagtaagac gagactccca ataaatttag cccgttttgc
    39661 atcactgcca acaacaccac gccgagcaaa gtaccggtta acgtgccgcg cccgccgagc
    39721 aaactagtgc cgccaagcac caccgcagcc agtactgtca gctcataacc cagaagagaa
    39781 tcgggggcga cagtcatcac cgtccacgac tgcactacac ccgcagcgcc agacatcaat
    39841 cccatatagc catagacgaa aagttgcagt ttcagcacgc taaagccgat gcgactggct
    39901 gattcgcggt tcccgccaag ggcataaatt ttgcgcccta cactggtgaa attcatgata
    39961 aacgctgtta gcagcaccac cgtaatcatg gcgatcagcg gcagaccaag gccatagtca
    40021 tagccatcag catcggtgta cttgaacaac ataaccccct gctcaaacca cggcggaaag
    40081 tcgtacagcc acacaccttt actcaaccat aacagcaggc catagaaaat attcagcgtc
    40141 gagatagtga tgatgataga aggcacccgt aggcgattaa ccagtaaggc gttaattaag
    40201 ccaagtaaaa tgccgatgca gcccgccagt gcaaacgcga cagcgaagtt gccaccaatt
    40261 ttttgcagca acagcaccat gccgtattgc gcaatgatgg tcattgctgg aaacgaaata
    40321 tcaattccac cggaaatcaa caccacaaac aaaccacagg cgagaatagt caacatggca
    40381 tagttattgg cgagatcgta caaattaccg aatgtcagaa actcgtcgct gcgccaggca
    40441 aggccaacaa acagcaccac aatcagtaat gccagccaga attcatggcg ttttttcagt
    40501 tcagccattt accacctccg caatcctggc ctgagaactg ctgtcaggca ggaagctatg
    40561 ggtgatctga cctttttgca ttaccaaaat ccggtggctt tgataccacg cttcttcaat
    40621 ttcatcgcag atcatcaata ccgcaatgcc atgagccgcc agatcgctga taatgccgta
    40681 tattcctgct ttgttggcga tatcaacacc gactgtcggg ctatcgagaa tcaacagtcg
    40741 tggcccaatc gccagccatt tggcgatcga tactcgctgc gcgttaccac cagaaagcgt
    40801 attcaccgga agatgaggat cagagacttt gatggtcaat gccttgatta gcctgtccac
    40861 caggtcacga actttagttt cactcagaaa gccgaagcga tcttttacct tgtgaaaaac
    40921 agtgctaatg atgttgtctt caatagattg cgccatcacc aaaccgcgcg acatgcgatc
    40981 ttccgaaaca taaccaatac cgtggcggat agcgtcctgg ttactatgca gtgtaaccgg
    41041 ctggccgttg atgaggatct cgccagcatc cggtcgggtc ataccaaaca gactcagaca
    41101 taattccgtg cgccccgcac caagcaggcc aacaatcgat accacttcgc ccgcttccac
    41161 ccgcaagtta atatttagat attccccatg acgacttaaa ttacgcacct ccagcaccgg
    41221 ggtgctggat tttccctgcc acaactctcg cacctgatat tcaaactcct ggccggtcat
    41281 caggaaaccg agttgttttg tggtcatctc tgcagccgga aaagtcccga ccagctcacc
    41341 atctttcagc actgaaatac ggtcggaaac ttccatcact tcttccagac gatgactgac
    41401 gaagaccacg cagatcccgc gttcacgcaa ttgatgcacc acctgtaata acccttgcac
    41461 ttcctgatgg gtcagcgaag cggttggttc atccatcacg atcaaccgtg cgtcctgcgc
    41521 cagtgcacgg caaatagcca ccagctgaca gcgggcaatt gaaagctcag aaacgagtgt
    41581 atccagtggc aatgtgacgt taatactggt cattgccgcc tgcgccacct cacgcagacg
    41641 acggcggtta acaaacagac cgtggtgata gtgattcacg gcgatattct cccagacact
    41701 caggttagga aataaagaga ggtcctgata aatcacctga atcccctgcg ccaccgaagc
    41761 ggcgggtgtc agctttgacc agtttttccc accaagcgta atttccgctc cctcatccgg
    41821 gcgataaaca ccggacataa ttttaatcaa cgtcgattta ccacagccgt tttgcccggc
    41881 aaggcagtgc acttcacccg acataaagtc gagagaaaca tcgcgcagcg ctcgcgtggc
    41941 atgaaacgtt ttgttgatat gacgaaggga aaggaaagtt tccataacat cccctggcaa
    42001 taagaatcgg ggctttcgcc ccgaagaaat ggattaataa agtgagtcga tattgtcttt
    42061 gttgaccagt aataccttat ggaaacggat aattcgctta tccatatcta cgtcggcctt
    42121 acccagattc tgcatttcta atccaggttt gatctcttca cccttcagca acgtgctggc
    42181 gacggctgcg agtgcgtaac ctgcagaggc cggatcgtaa gtaatccctt cggtgatatc
    42241 accactttta atcagtgatg ccgcctgtga agggatcatc atgccataga ctgcgacttt
    42301 atttttcgcc cgtttctctt tcaccgcacg tcccgcgcca atcggaccgt ttgaaccaaa
    42361 ggagacaacc gctttcaagt caggataggt tttcatcagg tccagtgtgg tacgacgtga
    42421 gtcatccaca ctctcggcaa ccggcatgcg gcgggtaact tcatgcatat ccgggtaatg
    42481 ctctttctgg tatttcacca gcaagtcagc ccataagtta tgctgcggca cggtcaaact
    42541 acccacgtaa atcacatagc cgcccttgcc ccccatgcgt ttcgccatat gctcaacata
    42601 ttcagcggca aatttttcgt tatcaatgat ttcaatatcc cagttagcac ttggctgacc
    42661 gggggattcg ttggtcagaa ccacaattcc ggcatctcgc gcttttttga ataccggttc
    42721 cagcacgttg gcatcgtttg gcacgatagt aattgcatta accttacggg cgattaaatc
    42781 ctcaataatt ttaacttgtt gcggagcatc agtacttgaa ggccccacct gtgaggcatt
    42841 aacaccaaag gctttacccg cctcaaccac accttcgccc atgcgattaa accacggcat
    42901 accatcgact ttagaaatat tcaccacgac tttttccgct gcctggagcg gcgcagaaat
    42961 tagcgcagcg cctaataaca gcgaagacac catattgata acaaaacgtt tattcatcat
    43021 ttcagcctca ttgcagggta gtcaggaagt gttcgcccaa attagcgcgc tggagagaat
    43081 aagaattttc gaatattttt atgcagaata aacagcccta tttcaggcag ctattaaaaa
    43141 tatggttata aatatggaca aaacgggcag caacaataag tgtgacattc atcacgtaaa
    43201 aaatagcaaa ataaacaaaa ttagcgatat tttatgtgtg tgagtttatt tcgcgtagat
    43261 aaatagttat ctacacaacg tcaataaggg catattcttc aaatataacg tccatttctg
    43321 gtgaatagct tccttctgcc tggaaaaaac gacggtgttc attacgccaa tattcaagac
    43381 tgaggtcacc ttcaccttcc ttacgggcaa gctccacggt gacatcagaa aagcgcagta
    43441 aatgcaggcc aatcactctg ataacacata caggctcacc acggctattt tctacaatct
    43501 tatactcacc aatcatcgga aatgcgttgt cttcaataca tcccgccagc gaaccacagg
    43561 aagctgtttt aacaccagtc gcaatcaacg ttgcgaggtg atcggccaac gctgctgaat
    43621 cgccaaaagt acaaaaggaa gcatggggat atttttgtga gaatttttct tttatggtca
    43681 tggcaatatt tttgcgatta cggaaaaaca aatcacatct taattactgt tggtaaccat
    43741 cagcaagagt atcattggtt tttcgatact gaatcttttt tatccgaagc aggtcacatt
    43801 tatggcctga taaatacctt tgttaaaact cacttttgtg tatttctctc aaataatccc
    43861 ctggaaatta tgccgcgaga ttataaagtt caggatcatt cattagcaaa ccattcccgc
    43921 cccactccct ggggcttaaa tctcttacaa ttcaccgttg caggcgtttc aaatgcgcat
    43981 caacctgcca cactccccca caccagttgc cctttatgga acgttgcggt tctcggcgag
    44041 atacgcgcca ccgcctcggc ggaacaggag gcgtccacca gcacaaagct ggcatcatcc
    44101 tgcgcttttg gccatacacg ctcgcctttt tcattcagcg gcaatacatc gccagtggct
    44161 agaaacagcg aacgggagag gttctgttcg ttaggacgaa tatagagctg cgcgtagaga
    44221 ttggcttttt ccagcatgtc gcccagacca taaggcgacc agtggtcgat aacgctgtca
    44281 gtgccagtca tcacttttac gcctttgtcg tgcaactgtt tgagcggcat atgcagcgtg
    44341 ccaatcggca ccgtcgaggc gatagaaatt tgctgcgccg ccatccgatt cgccagcgca
    44401 tcgacctgtt gttcgttgag cgtagccagc gcaaaggcgt ggctgatggt caacttacct
    44461 ttcagttgcg gcgttttctc taccgtttca accatataat tgatggctgc cacgcccgac
    44521 ggagtggttt cgtgcaggtg aatatcgaca cctttgtcgt agtccagcgc aatctgaaac
    44581 atggtgtcga gggatttttc catcgcgcca tcaacactgg tcgggtccag cccgccgacg
    44641 taatgcgcac ccgcctgcat cgcttcgcgc attaagggtt ccgatttcga cagcagcaaa
    44701 ccgtgctgcg ggaaggcgac gatttcacac tcgaagcccg cctgacgccg cgccagcacc
    44761 gcctgcaaat tttgcagatt ttttaggccg gaaaccggtt cgatattgca gtggctgcgg
    44821 gcaatggtgg tgcctttcga ctgcaataga tcgatcagtt tttccgcccg ctcctgcgta
    44881 tacggttgta actccggcag cattttctgc tcgagtttga tcatgtcctg gatagtggtg
    44941 cctgccggac gattgagcga gcgccacggc ccgccgtaga aggttttgtc gagatgaata
    45001 tgcatgtcgc gagtggtggg tagcatcaac ttaccgccag cgtcatagtg cggcagcgtg
    45061 gcgtccggat gctgcttgtt ctcgcgcagg gcgacaattt tgccgtcctg aatctccacg
    45121 gtctggcgcg ccgtgcgggt ctgcaccgcc acaccgtttt cgtagtcaaa accggtttcc
    45181 agcagcacgt tatcgagata gtaatgcggg tcagtgattt tcatggtgtt gttcgcttcg
    45241 cagtttaggg tgccagctac cgccgcatgg gcgagcggca cagaggtacc aaacagcgcg
    45301 gcggcggtga ccatcttacc gctctggctc agaaattcac ggcggctatt gctttctttc
    45361 atcctacgtc cttacgtttt aataatatgg gtgcccgttt caccgcgcag cgccgcaggc
    45421 aggcattccg gcgtggtgat aatcacttct ttgccgccct gctccaggaa cgtcaggctg
    45481 gcgatgattt ttggcaacat gctgccgggc ggaaaatgcc cttcctgcat atagcgagtc
    45541 atggtggcaa tatccacccg atccagcgcc tgctgctgcg gtttaccaaa gtggatacag
    45601 actttttcga cgccggtggt gatcaccaga atgtcggcgt gaatttcacg ggccagcagc
    45661 gcggtagaga gatctttgtc aataaccgcg tccacgcttt ggtaatcccc cgcttcagta
    45721 cgcactaccg gaattccacc gccgcccgcg ccaattacga caaaaccttg ttggatcagc
    45781 gccttaatgg cgggtgcttc gacaatacgt ttcggttccg gcgaggcgac cacgcggcga
    45841 tagccccgtc cggcatcttc aacaaaacac cagtcagggt ttgccttttg tagtttgtca
    45901 cgctggcttt cactgaagaa tgcaccaatg ggcttggtgg gatgggcaaa acctggatcg
    45961 tttttatcca cttccacctg agttaccacg gtgacggctt ttttctcgcc gtgacgcgcc
    46021 agccggttat tcagcgcctg ttggatcaga tagccgatgc cgccctgcgt atctgccaca
    46081 cagttcgcca gcggcgttaa gggcagccct tcgcgctcgt gggcaatctc cgcacggcgt
    46141 aaatccagcc cgacctgcgg cccgttaccg tgggtcagca caatgtcgta atcggaagcc
    46201 agcatttcca gcaccgtatc ggccacggct ttcaccgcct ccgcctgatg ctcaatcgac
    46261 tggctggcgt tatctttgat aatgctgttg ccaccaatgg caacgaccac aagctctttc
    46321 atgttgttat gtccgctaca gagggtgatt aggttatgac caacgccaga ctgcctgatg
    46381 cgctacgctt atcaggccta caattctcgg gccatccgta ggccggataa ggcgttcaca
    46441 ccgcatccgg cataaacatg taagtcagac gccgtaacgt tcgcaccagc cgagaatggc
    46501 tttctcgaag caggcgaatg gtggatgcac aatgcccgcg ccaatcatgc cgacgcccgc
    46561 atctttatgg gcgatggcgg tgttgatcac cggcagaatg ccgctgctac ccacgcgggt
    46621 gatgtcgatt gccgacggca cgcccataaa accgagcagc ggaatggtga cgttggggtt
    46681 ttcgccaagg gtgatttcgc gcatctgacg ggagaaatcg atagcctctt ccaccgtgcc
    46741 gcccaccagt gcgacgattg caggcgcggt cgccatggca aacccgccaa taccgtaggt
    46801 ttctgtgatg gcgctgtcgc cgatatccag ccccgaatct tccggcttat aaccagcaaa
    46861 catcgggccg atgacctgct gcgctgggcc ggtaaaccac tgccccggca ggccgctgac
    46921 ccgcaggccg aactcgacgc cgttacgcgc catggtggtg accacggtgc tgtattcgat
    46981 gccgtgcgcc gcatccatcg ccgctttaca catcgccatc cacgtcgggc cggagaagta
    47041 gtcgctgctg gcgacaaact cgaacacttc gcgctgttgc tcgacggaat agcctgcctg
    47101 aatgatcccc ggcgtcagtg cctgaatcag cagcgtcgtc ccggcgttat tgcggttatg
    47161 gcactcatcg cccatatgca gcgcctgcgc cagcattaag cgcagatcga tctcaccgat
    47221 aattttcatc gcgtcgcgca gcattggccc ctgcacatcg cgcatccagt tgaggcggtc
    47281 gatcacgctc tgatcgttgg cacccatacg caaaatcttc gccatctgct cgctcatatt
    47341 ggtataagcg atgttgccgt aggttttgtt tttcacgatg tgcataaaca tcgaggccga
    47401 ggtaacgccc gccatcgatc ccacgcagtc gtgttcgtgg cacggcgaga aggtgatctc
    47461 cccggaggcc gccagttcag tcgcctcatc gagatctttc gccagccctt cgaacaccaa
    47521 cgccccggtg accgcgcctt tcatcgcgcc gcacattttt tcccaggtga tcggcggccc
    47581 ggcgtgaaga atggttttcg gcgtcatgcc cggcaccacg ttaatcgcct gatcaaaacc
    47641 aatcagcacc ggatgcgact ggataatgcg ctccagcgcc tgctggttag cggcggcgat
    47701 tttgtctgcc agcggcgaat cggcaatgtt atccagcgcc tgcaccacct gcatattgcc
    47761 ctgccccggc ggcgtccagt cgagttgagt cacttctaca tgctgctttt tcaggtcatc
    47821 gctaaacatg gcgataccga cgttaataac gttcaacggt tggctaaaca gtgactggct
    47881 catcaggctt cctccccttt gcagataaat tcacgcgcca gcaatccggt attggtgctg
    47941 ctgctcgcca gaatcactcc ggcatcaagc agcatctggc tttgttgttc taatgatggc
    48001 gtatcgagat cggtgcccag cacataggcg agaatgatca actcgcgtcc ttcggcggcg
    48061 gcgatcgctt tcgcttcttt gatcgcctcg atggtggagc cgaccggatc ttcatgcgat
    48121 ccaaatccga gcacaaaatc catcacgatc accgccactt ctggatcgcg cgcctcttcg
    48181 atcaagcgac tgatgcggtt ggtggggtca atcattggat gcggcttgcc gttggtgaag
    48241 tcgtcatcgc caaagtcgag gaaggtgtgt ttgatgctac ggttgatatc ttgcaggcgg
    48301 aattccggat cgggctgaat gttgctgtag acatcgccgt gtttttccat caccgcgaac
    48361 atggtttcgt cacacagcgt gccgccacag aacaggccac gaatgtactt ctgctgcggt
    48421 tgcaggcgag cacgtacgtc agcaatcaac ggctggttca gcgtatgcag gtcgagatgt
    48481 tcctgtttca cgccggagag catcaccgct ttcaatgccg cctctttgct gccgcgggca
    48541 aactgtagcc cctgctcatc cactggcgtt tcgccacgac cgaggaaaca gaccaccacc
    48601 ggcttgcggc aggcgcgcgc gcgttccagc actttgcggg ccaccgcagg cgcgggcggt
    48661 ttggagataa gcacaatgat ttcagtttgc ggatcgtttt ccagcatgcc gatggcgtcg
    48721 agcatcatca ggccgccgat tttctcgctc aggtcgcgcc cgccggtgcc aatcagttgc
    48781 gaaacgccgc cgccaaattc atgaatgcgg acgctcagct cctggctgcc ggtgccggat
    48841 gcgccaacaa taccgatgtt gccgcgacgc acggcgttac caaaacagag cgccgcgccg
    48901 ttaataatcg ccgtgccaca gtctggcccc atcatcagca gccctttttc gtgggccagt
    48961 tgcttgagcg ccaattcatc ttcaactgag acgttatcgg aaaacagcat cacgttgaga
    49021 tcgttttgca gcgcctgacg cgcttcgcga gcggcaaaca gaccgttgac cgaaatcacc
    49081 gccaggttac tttccgggat atgctttttg gcgctggcaa tagtggcgta acgcgcctca
    49141 tgcgagccgc tttgcgcttt ggtgttgaac agttcttcaa tctccaccag caactgttcg
    49201 ttgtccgcgc ccgctttgcc gttgatgaca atcatcaggt cgccgttttt cgcctgttcc
    49261 agctccggcg tcagcagtcc taaatttttc agtacacctt tgttcatttc ggtcgccatc
    49321 gccacaaatg cctgctcgac gccgtcgagt ttatttgcac gcgtggagat agacatcagc
    49381 gagacagaat caaaataggt attcggttta atgactattt taactgacat aattccctcc
    49441 gtaggtttgg ctcagcgcac aaccatcggc catgccatac agcatgtcgc agccggaact
    49501 ggagccaata tgtttaattt tttctattgc ctgagttgcg ttcccaggga tgtcataaat
    49561 aatgttgtga ataaaacgat gaatattttc ccggcaacgt tgttgtaatg cggcttccag
    49621 cgttatagca cttaataatg tggtgttatt tctgccgcgc tgcataccga gataaaattc
    49681 ctctttgtat ttttccgacg gatgcccggg aataaataaa ataagcgcca gacctgttaa
    49741 ataatcgtcg gctgatggcg taaggccaat ccctaaccca atcatactgg cgaccgctgc
    49801 ggcgatattt tgtttttcgt ttactgcctg aataactgcc cgtcgtttaa tatgtagttc
    49861 tttatttaac gcctgataaa acggattatc gccgcgataa ttaaacaggg tttcattttc
    49921 ccccaggtgc tgatgaataa tgtcgtgcca ctgtagccag gcgataaggt gaaaattcac
    49981 agcggtcatt tgcaaggttg gggacagcca acgccgacaa cgactcatct ctatatgaag
    50041 atgttgacca accgtaatac cttgatcgtc aaactgaacc ttatcaccat gccggaacag
    50101 atcatcaaag tgagtgagtg ccaaccgaca actgtttggc gcattgtcgt actcttcaca
    50161 aagcagagtc agcaactgct ggcgagccgg gagtgcgata tttaccgctc tggcaaagac
    50221 ctgttcaacc tgtccttgcc cccgctgcgc cagaaaatcc gcatccgcag ttagcgccca
    50281 cacacaccgt ctttctggtg tgttgatagc aagcctcccg gtttatgcac ctgcggcaat
    50341 cagtaactgc gcaatctctt caaagccccg ttcccgcgcc agttccagtg gcgttttgcc
    50401 atatttatcg gtcagatgcg ggctggcacc gtgttccagc aataactgca caatcgcctg
    50461 ctgtttaata ccaccatcat taagcacaat cgcttccagt agcggcgtcc agcctacatg
    50521 gttggtctgg ttaacgttaa tctccgtatg cgccaaaagc tcttttacaa tacttaaatg
    50581 gcctttttca caggcaggcg tcaggccgac accgccaaaa cgggttacgc aattaagatc
    50641 gggtttagcc ggtaaaataa ttcgtagtag cgttaaatca tcgttcagac agctaattaa
    50701 aaaaggattt aaacaggtat gatcttgctt attaatatcc gctccggcat caattaatgc
    50761 ctgaacgcaa gcatattgct gatataaact tgccagcgta attgccgttt tcccctgacg
    50821 atcgcaggta ttaatatcga cacccagcgc aaggcaggtt tttactttat cgatatcacc
    50881 ctgttgtgca gccaataaat agtcggcagg tagatttttt atactcattc acattcccct
    50941 ggtttttgtg gctgttttcg atgaattgag aatagcaacg ataatctgat aaaagaaccc
    51001 gcttaaattt aattcgctgt atttcgcctt tttgttgaat aatgttcaac aatcttttcg
    51061 cagtgtgcgg tagtgcaata tttgtttctt tgacccgata ccctttcact caatcaggtt
    51121 ttatttctct atagtctgca catgtaagag tagctttcgt ccatgtctaa ccagagggga
    51181 acaagatgaa cggactcacg gcaacgggtg tcaccgtcgg tatttgtgca gggctttggc
    51241 agttagtgtc ttcacatgtc gggctctctc aaggatggga attattagga accattggct
    51301 tcgtcgcctt ttgcagtttt tatgccgcag gcggcggtaa atcggggttt atcagaagtc
    51361 tggcggttaa ttattccggg atggtgtggg cgtttttcgc cgcgctgact gctggttggc
    51421 tggcctctgt aagtggactg tccgcgttct gggcgagcgt aattactacc gtacccttct
    51481 cggcggtagt ggtctggcaa gggcgtttct ggttactgtc ctttattccc ggtggctttc
    51541 tcggcatgac gctgtttttt gccagcggga tgaactggac ggtgacgtta ctgggttttc
    51601 tggcgggtaa ctgcgtgggg gtaatttcag aatatggtgg acaaaaatta agcgaagcga
    51661 cgactaaacg cgacgggtat taaaagttat tcaaaaatga ttatctatct ttaaccgata
    51721 cctctacagt catagctgga gaaattcata gtagataatg gcgagaatgg tttaatgaac
    51781 tcaattttta ccgaagagaa tctgttggca tttactaccg ctgcgcgttt tggcagcttc
    51841 agtaaagccg cagaagagtt gggtttaacc acttccgcca ttagctacac cattaagcgt
    51901 atggagacgg ggctggatgt agtgctgttc actcgcagta cccgcagcat tgagttaacg
    51961 gagtccgggc gttatttttt ccgcaaagct accgacctgc tgaatgattt ttatgccatc
    52021 aaacgtagca tagatactat ttcgcagggc atagaagcgc gtgtgcgcat ttgtattaat
    52081 cagctgcttt atacgcccaa acacaccgcg cgcttattac aggtgctgaa aaagcagttt
    52141 cctacctgcc agattaccgt caccaccgaa gtgtataacg gcgtctggga tgcgattatt
    52201 aataatcagg ccaatatcgc catcggcgca cccgatacac tactggacgg cggcggtatt
    52261 gattacactg aaattggcgc gattcgctgg gcttttgcta tcgccccgga tcacccgtta
    52321 gcctttgtgc cggaacccat cgccgaaagc caactgcgtc tctaccctaa tattatggtg
    52381 gaggacaccg cgcatacgat taataaaaaa gtgggctggt tgctgcatgg gcaggagtcg
    52441 attctggtgc cagatttcaa caccaaatgt cagtgccaga ttttaggtga agggattggt
    52501 tttttgccgg attacatggt ccgtgaggcg atggcgcaat ccctgctggt tacccgacaa
    52561 atccataatc cgcgccagga ttcgcgcatg ttgctggcga ctcagcattc tgctacgggt
    52621 caggtgacgc agtggattaa aaaacaattt gcgccaaatg gcatattaac ggggatctat
    52681 caggatttac tgcatcggga gaattaatcg ctgccaacct cccgatgcgc tgtgaatagc
    52741 ggtttacatc aaccacctgc tttcattacc cattcagaga taaatttatt acccggaact
    52801 ggcggggagt acaaatagcc ctgcaaaaag tgaacgcctt taccgatcat taaatccgcc
    52861 tgctcctggg tctctacccc ttccgctacg atactcagac caggcttacg cgccagttcg
    52921 acaatattgt ccacaatatg accggagatt tcgtcgacac tcgccatttg cacaaatgac
    52981 ttatcgatct taataaaatc gaccggaaac gcctgcagat aacgataggt cgcataaccc
    53041 gtaccaaagt catccagcgc aaaggtaatg ttgtgctgat gaaggctgtc aaatatcgct
    53101 ctggcttctg gcgttaccgg aatagggtta cgttccgtta gctcgagaac cagtttgatt
    53161 ttatcgtttc ctaatttctt gaccagattc aggcattctt tttcaaatcc cgcggccaaa
    53221 aaacaacccg ccgagacgtt gataccaata tggaaattat ccggcagcaa atgttttacc
    53281 ggcatcagaa tatccgcagt ctgtttcatc agttggcggg tcattatgac aataagaccg
    53341 gatgactccg ccagaggaat aaactgatcc ggtgggataa ttcccgtttg tggatgttcc
    53401 cagcggacaa gcacctcaca gcccgtcagt accccagtct gcgcgcagaa aaccggttgg
    53461 atccacggtt tgaattcgtg gttttccaac gccagactga tggcttcagg cgtgacgata
    53521 tggtgatagt gattatcatt aatatatcta tgactattac tacccgtggc ggatatgatc
    53581 tccggattgt actgaaagaa aatatcgcgc cagaaagtaa tatcgctctg aatccccagt
    53641 ttctcaaaga gctgtttctt ttgatgtgaa attgtctttg cactgcgatt tctgtactgt
    53701 gatatttctg ttacagacat tccctgcatc agcagcttga gtagcagtcg ttctttctcg
    53761 gatacgctac caggcaatga caaatgaacc gttgtaccga actcttgcag aaaaacacga
    53821 aaatcacatg aattcatgaa cacaccttta tcttttatcg ctgttcagta tgagtgatta
    53881 cctgtggatt cctgagcaca cataaggggt aaccataaac attagcgatg ccatacgtat
    53941 aacatcccac atcactatca aaaagattat ctttacctct gatgctcgct caaaaacagt
    54001 tacgctaaat accttaaaaa ggtaatcaca agacaaactc aggagataat cacgcaagaa
    54061 tgcaatatta aaattcagat actcattttt ggtaataaag aagtagttgg actatttcaa
    54121 tatttataat ttcccactac gtactggcat ccattcatga gcaaaggcgc attatatgaa
    54181 tttaacaatc cagatcaact gaaaatacct ctccctcata aacacatagc gtcaacattc
    54241 aatgacataa tgagtaaaga tgttggttat gcatacgtat cattactcta tgcctgtccc
    54301 ttaaaaaccc acccattaag actgaatcca ttcagcaaat g
//
LOCUS       DAZFRN010000012        53645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFRN010000012 DAZFRN010000000
VERSION     DAZFRN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.012"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(50..1414)
                     /gene="mnmE"
                     /locus_tag="ACHKUB_000694"
     CDS             complement(50..1414)
                     /gene="mnmE"
                     /locus_tag="ACHKUB_000694"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO6653756.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1520..3166)
                     /gene="yidC"
                     /locus_tag="ACHKUB_000695"
     CDS             complement(1520..3166)
                     /gene="yidC"
                     /locus_tag="ACHKUB_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO6653757.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3169..3426)
                     /gene="yidD"
                     /locus_tag="ACHKUB_000696"
     CDS             complement(3169..3426)
                     /gene="yidD"
                     /locus_tag="ACHKUB_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO6653758.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3390..3749)
                     /gene="rnpA"
                     /locus_tag="ACHKUB_000697"
     CDS             complement(3390..3749)
                     /gene="rnpA"
                     /locus_tag="ACHKUB_000697"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO6653759.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3766..3906)
                     /gene="rpmH"
                     /locus_tag="ACHKUB_000698"
     CDS             complement(3766..3906)
                     /gene="rpmH"
                     /locus_tag="ACHKUB_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO6653760.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4136..4216)
                     /locus_tag="ACHKUB_000699"
     CDS             complement(4136..4216)
                     /locus_tag="ACHKUB_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653761.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4513..5916
                     /gene="dnaA"
                     /locus_tag="ACHKUB_000700"
     CDS             4513..5916
                     /gene="dnaA"
                     /locus_tag="ACHKUB_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO6653762.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5921..7021
                     /gene="dnaN"
                     /locus_tag="ACHKUB_000701"
     CDS             5921..7021
                     /gene="dnaN"
                     /locus_tag="ACHKUB_000701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO6653763.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7021..8094
                     /gene="recF"
                     /locus_tag="ACHKUB_000702"
     CDS             7021..8094
                     /gene="recF"
                     /locus_tag="ACHKUB_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO6653764.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRFLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8123..10537
                     /gene="gyrB"
                     /locus_tag="ACHKUB_000703"
     CDS             8123..10537
                     /gene="gyrB"
                     /locus_tag="ACHKUB_000703"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO6653765.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10777..11175
                     /gene="yidB"
                     /locus_tag="ACHKUB_000704"
     CDS             10777..11175
                     /gene="yidB"
                     /locus_tag="ACHKUB_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO6653766.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11290..12102
                     /gene="yidA"
                     /locus_tag="ACHKUB_000705"
     CDS             11290..12102
                     /gene="yidA"
                     /locus_tag="ACHKUB_000705"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO6653767.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12148..12804)
                     /gene="yidX"
                     /locus_tag="ACHKUB_000706"
     CDS             complement(12148..12804)
                     /gene="yidX"
                     /locus_tag="ACHKUB_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO6653768.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13082..13771
                     /gene="dgoR"
                     /locus_tag="ACHKUB_000707"
     CDS             13082..13771
                     /gene="dgoR"
                     /locus_tag="ACHKUB_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO6653769.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13768..14646
                     /gene="dgoK"
                     /locus_tag="ACHKUB_000708"
     CDS             13768..14646
                     /gene="dgoK"
                     /locus_tag="ACHKUB_000708"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO6653770.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHWLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14630..15247
                     /gene="dgoA"
                     /locus_tag="ACHKUB_000709"
     CDS             14630..15247
                     /gene="dgoA"
                     /locus_tag="ACHKUB_000709"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO6653771.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15244..16392
                     /gene="dgoD"
                     /locus_tag="ACHKUB_000710"
     CDS             15244..16392
                     /gene="dgoD"
                     /locus_tag="ACHKUB_000710"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO6653772.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16467..17804
                     /gene="dgoT"
                     /locus_tag="ACHKUB_000711"
     CDS             16467..17804
                     /gene="dgoT"
                     /locus_tag="ACHKUB_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6653773.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFASGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17801..18865)
                     /gene="cbrA"
                     /locus_tag="ACHKUB_000712"
     CDS             complement(17801..18865)
                     /gene="cbrA"
                     /locus_tag="ACHKUB_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO6653774.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            18966..20180
                     /gene="yidR"
                     /locus_tag="ACHKUB_000713"
     CDS             18966..20180
                     /gene="yidR"
                     /locus_tag="ACHKUB_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO6653775.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20182..20514)
                     /gene="yidQ"
                     /locus_tag="ACHKUB_000714"
     CDS             complement(20182..20514)
                     /gene="yidQ"
                     /locus_tag="ACHKUB_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6653776.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20754..20826
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20820..21233
                     /gene="ibpA"
                     /locus_tag="ACHKUB_000715"
     CDS             20820..21233
                     /gene="ibpA"
                     /locus_tag="ACHKUB_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO6653777.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21345..21773
                     /gene="ibpB"
                     /locus_tag="ACHKUB_000716"
     CDS             21345..21773
                     /gene="ibpB"
                     /locus_tag="ACHKUB_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO6653778.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21970..23631
                     /gene="yidE"
                     /locus_tag="ACHKUB_000717"
     CDS             21970..23631
                     /gene="yidE"
                     /locus_tag="ACHKUB_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO6653779.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23628..24344)
                     /gene="yidP"
                     /locus_tag="ACHKUB_000718"
     CDS             complement(23628..24344)
                     /gene="yidP"
                     /locus_tag="ACHKUB_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6653780.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSLMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24640..26256
                     /locus_tag="ACHKUB_000719"
                     /pseudo
     CDS             24640..26256
                     /locus_tag="ACHKUB_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            26256..27070
                     /locus_tag="ACHKUB_000720"
                     /pseudo
     CDS             26256..27070
                     /locus_tag="ACHKUB_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27067..27960)
                     /gene="yidL"
                     /locus_tag="ACHKUB_000721"
     CDS             complement(27067..27960)
                     /gene="yidL"
                     /locus_tag="ACHKUB_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6653781.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28126..29841
                     /gene="yidK"
                     /locus_tag="ACHKUB_000722"
     CDS             28126..29841
                     /gene="yidK"
                     /locus_tag="ACHKUB_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO6653782.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29838..31331
                     /gene="yidJ"
                     /locus_tag="ACHKUB_000723"
     CDS             29838..31331
                     /gene="yidJ"
                     /locus_tag="ACHKUB_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6653783.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31378..31827)
                     /gene="yidI"
                     /locus_tag="ACHKUB_000724"
     CDS             complement(31378..31827)
                     /gene="yidI"
                     /locus_tag="ACHKUB_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO6653784.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31937..32284
                     /gene="yidH"
                     /locus_tag="ACHKUB_000725"
     CDS             31937..32284
                     /gene="yidH"
                     /locus_tag="ACHKUB_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO6653785.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32274..32636
                     /gene="yidG"
                     /locus_tag="ACHKUB_000726"
     CDS             32274..32636
                     /gene="yidG"
                     /locus_tag="ACHKUB_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO6653786.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32633..33130
                     /gene="yidF"
                     /locus_tag="ACHKUB_000727"
     CDS             32633..33130
                     /gene="yidF"
                     /locus_tag="ACHKUB_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO6653787.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRAVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33138..34322)
                     /gene="emrD"
                     /locus_tag="ACHKUB_000728"
     CDS             complement(33138..34322)
                     /gene="emrD"
                     /locus_tag="ACHKUB_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO6653788.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            34404..34478
                     /gene="ysdE"
                     /locus_tag="ACHKUB_000729"
     CDS             34404..34478
                     /gene="ysdE"
                     /locus_tag="ACHKUB_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO6653789.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34741..34830)
                     /gene="tisB"
                     /locus_tag="ACHKUB_000730"
     CDS             complement(34741..34830)
                     /gene="tisB"
                     /locus_tag="ACHKUB_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO6653790.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35395..35493
                     /gene="ivbL"
                     /locus_tag="ACHKUB_000731"
     CDS             35395..35493
                     /gene="ivbL"
                     /locus_tag="ACHKUB_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO6653791.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35599..37287
                     /gene="ilvB"
                     /locus_tag="ACHKUB_000732"
     CDS             35599..37287
                     /gene="ilvB"
                     /locus_tag="ACHKUB_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO6653792.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37291..37581
                     /gene="ilvN"
                     /locus_tag="ACHKUB_000733"
     CDS             37291..37581
                     /gene="ilvN"
                     /locus_tag="ACHKUB_000733"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6653793.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37656..38246
                     /gene="uhpA"
                     /locus_tag="ACHKUB_000734"
     CDS             37656..38246
                     /gene="uhpA"
                     /locus_tag="ACHKUB_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO6653794.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38246..39748
                     /gene="uhpB"
                     /locus_tag="ACHKUB_000735"
     CDS             38246..39748
                     /gene="uhpB"
                     /locus_tag="ACHKUB_000735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO6653795.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLSLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39758..41077
                     /gene="uhpC"
                     /locus_tag="ACHKUB_000736"
     CDS             39758..41077
                     /gene="uhpC"
                     /locus_tag="ACHKUB_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO6653796.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            41215..42606
                     /gene="uhpT"
                     /locus_tag="ACHKUB_000737"
     CDS             41215..42606
                     /gene="uhpT"
                     /locus_tag="ACHKUB_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO6653797.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42651..44417)
                     /gene="adeD"
                     /locus_tag="ACHKUB_000738"
     CDS             complement(42651..44417)
                     /gene="adeD"
                     /locus_tag="ACHKUB_000738"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO6653798.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            44592..45926
                     /gene="adeQ"
                     /locus_tag="ACHKUB_000739"
                     /gene_synonym="yicO"
     CDS             44592..45926
                     /gene="adeQ"
                     /locus_tag="ACHKUB_000739"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO6653799.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            45979..46431
                     /gene="yicN"
                     /locus_tag="ACHKUB_000740"
     CDS             45979..46431
                     /gene="yicN"
                     /locus_tag="ACHKUB_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO6653800.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            46642..47832
                     /gene="nepI"
                     /locus_tag="ACHKUB_000741"
     CDS             46642..47832
                     /gene="nepI"
                     /locus_tag="ACHKUB_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO6653801.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(47873..48166)
                     /gene="yicS"
                     /locus_tag="ACHKUB_000742"
     CDS             complement(47873..48166)
                     /gene="yicS"
                     /locus_tag="ACHKUB_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO6653802.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            48388..49206
                     /gene="nlpA"
                     /locus_tag="ACHKUB_000743"
     CDS             48388..49206
                     /gene="nlpA"
                     /locus_tag="ACHKUB_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO6653803.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(49210..50133)
                     /gene="yicL"
                     /locus_tag="ACHKUB_000744"
     CDS             complement(49210..50133)
                     /gene="yicL"
                     /locus_tag="ACHKUB_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO6653804.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LSFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(50244..51422)
                     /gene="setC"
                     /locus_tag="ACHKUB_000745"
     CDS             complement(50244..51422)
                     /gene="setC"
                     /locus_tag="ACHKUB_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO6653805.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLGYYMK
                     RIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDLMP
                     EKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFCLAIGMLGIAMICLLFIKDI
                     "
     gene            complement(51819..51980)
                     /gene="rhuM"
                     /locus_tag="ACHKUB_000746"
                     /pseudo
     CDS             complement(51819..51980)
                     /gene="rhuM"
                     /locus_tag="ACHKUB_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(52217..53059)
                     /locus_tag="ACHKUB_000747"
     CDS             complement(52217..53059)
                     /locus_tag="ACHKUB_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6653806.1"
                     /translation="MSEITAPRPEVVNGHTDVICSTSIRHILAVQKSTLLQIDTLTRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARETWYRSLEYDNFPEISEANILSTFKQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIINNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSVQGKECYEDEMFIIRYFKKGSAHIRFRKPELVDRLNDIIAKHYPG
                     VLPS"
     gene            complement(53144..53341)
                     /locus_tag="ACHKUB_000748"
     CDS             complement(53144..53341)
                     /locus_tag="ACHKUB_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6653807.1"
                     /translation="MMPLTTETALDILIAWLQDNIDCESEIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLYQNR"
     gene            complement(53417..53566)
                     /locus_tag="ACHKUB_000749"
                     /pseudo
     CDS             complement(53417..53566)
                     /locus_tag="ACHKUB_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            complement(53563..>53645)
                     /locus_tag="ACHKUB_000750"
     CDS             complement(53563..>53645)
                     /locus_tag="ACHKUB_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFO6653808.1"
                     /translation="ATGLMTRDNYRMVNDISQGKHPEAKQ"
BASE COUNT        12542 a        13497 c        14358 g        13248 t
ORIGIN      
        1 atgaagcgaa gccgcatctg acagtcagaa tacggcttcg taagcgcggt tacttaccaa
       61 tacagaagct ggagaaaatc cgccccagca ggtcatcaga agtaaattcc ccggtgattt
      121 cgcttaagtt ctgctgtgcc aggcgcaact cttccgccag cagttcgccc gcccaggcac
      181 ctaacagttg cgctttacct tgctgcaaat gctctgccgc ctgttccagc gcctgtaggt
      241 ggcgacggcg cgccaggaag ccgccttcca tgttggtgtc aaagcccatg ctctgtttga
      301 gatggttacg cagcacatcc acaccttcac cagtccttgc cgagagacga attaacgcgt
      361 gaccgttcac ttcactcatt cccagcgttt cgccggtgat atcggcttta ttgcgcacca
      421 cggtgatcgg cagtttcgct ggcagacggg cgataaattc cggccagatc tctgccggat
      481 caacggcgtc tgtcgtggtg ccatcgacca taaacagcac gcggtcggcc tgttcaattt
      541 cctgccacgc gcgctcgata ccaatacgtt ccacttcgtc actggcttca cgtagcccag
      601 cggtatcgat gatatgcagc ggcattccgt caatgtggat atgctcacgc agcacgtcac
      661 gtgtggttcc ggcgatatca gttacgattg ctgcttcacg gcccgccagc gcgtttaaca
      721 ggctcgattt accggcgtta ggacgtccgg caatcaccac cttcatccct tcgcgcaaca
      781 aactaccctg acgtgcttca gcacgcactg catcgagttc ggcaatcacg tcattaagct
      841 gggcttcaat tttgccgtcg gagaggaaat cgatctcttc atcagggaaa tcaattgccg
      901 cttcgacgta aatgcgcaag tgggtgaggg cttccaccag atggttgacc cgtgcggaga
      961 atgcgccttg cagcgagtta agtgccgaac gggccgcctg ttccgaactg gcgtcgataa
     1021 gatcggcaat cgcctcggcc tgggctaagt cgagtttatc gttaagaaac gcgcgttcgg
     1081 aaaactcacc agggcgagca atccgcaggc cgggaatggt cagaatgcgt tttaacagca
     1141 ggtcgaggat caccggaccg ccatgacctt gcagttccag cacatcttcg ccggtgaacg
     1201 agttcgggcc agggaaccat agcgcaatcc cctgatcgag cacgctgccg tcggcgtctt
     1261 taaacggaag ataatcggcg tagcgcggct taggtagttt acccagcacg gtttcggcaa
     1321 cttcacgggc tttgaagccg gagatgcgca ggatgccaac gccgccacgt cccggaggcg
     1381 tggcctgggc tacgatagtg tcattatcgc tcatgatgtt cctgttgctt tgtgtggcgg
     1441 atgcgcggtg cttatccgcc ctacgaaaag aaaaaaggcg gtcaactgac cgcccttatt
     1501 ttagcgaaaa ctcaccgaat caggattttt tcttctcgcg gctatgcagg ccacgttttt
     1561 ccagaccacg gtaaatcagc tgctgctgaa taatggttac caggttgctg acgatatagt
     1621 acagcaccag acctgacggg aaccacagga agaacacggt gaagatgacc ggcataaagg
     1681 tcatgatctt ctgctgcatc gggtcggtca ctgtggtcgg cgacatcttc tgaatgaaga
     1741 acatcgttac gcccatcagg atcggcaaga tgtagtacgg gtcctgtgcc gacaggtcat
     1801 ggatccacag tgcaaacggt gcctgacgca gttcaacgga acccatcagc atgtagtaca
     1861 acgccaggaa gattggcatc tggatcagca gcgggaagca gccgcccagc gggttaacct
     1921 tctcagcttt gtacagcgcc atcatttcct ggctgatacg ctgtttgtca tcacccagac
     1981 gctcacgcat tgcctgaatc ttcggctgca gcatacgcat cttcgccatg gaggtgtact
     2041 gcgctttggt cagcgggtac atgatgccac gaacgataaa ggtgatgatg ataatggaga
     2101 agccccagtt acccacaaag ctatggatcc atttcagcag tttgaacagc ggctgagaga
     2161 tgaaccacaa ccaaccgtaa tcaacggtca gatccaggtg cggagcaaca gctgccattt
     2221 tgtcctggat ttccgggcca acccacaggg tgctgttcat cgcgccagtc tgaccaggct
     2281 gaaccagtac cggctgagat ttatagccga tagcggcgat gccgttaccc agattagcgg
     2341 tatagaagtt gttggtaccg tcgttatgcg ggatccacgc cgtcgcgaaa tactgttgca
     2401 gcatcgccac ccaaccacct ttcgaagaga tgttcaggtt ttcgttatcg gcaatggtat
     2461 cgaacttgta tttctcatac ttctcgtcag gcgtggagta cgccgcgcca cggaaggtgt
     2521 gcagtgcgaa gttgctgctt ccggtatcga gatgcggtgg cagagtgatg gattgcttca
     2581 actgaccaaa ggtggagatt tccagcggtt tctcgccagc gttctgcacg ttgtagttga
     2641 cgttgacagc gtaatcacca cgtttcagga caaacgtttt ggtaaacgtg ttgcctgccg
     2701 cgtcggtata cgtcatcggc acctgcagtt cgttttgacc ttcagccagc acataagcgt
     2761 ctttttcaac gttatacagc ggacgcgggc cgttagccgg gttatccggg ccatcacgac
     2821 cggtcagacc gctctgtgcc tgataaataa actgcggtga agtttccagc agctggaacg
     2881 gctgggtaga gttcagctct ttcgggtaag ccggcagcag agcttgctca acatcaccac
     2941 cacgggtgtt gatggtcaga tcaagcacgt cggtcttaac cgagatcagt ttcccctggc
     3001 cactggccgg tacgccctgg tcggcggcgc tacccgctgc ggtggtcgtt gtctgcgtgg
     3061 tctgttgggc ctgaggttgc gggtttttat cctgctccca ggcttgccag atcatgaaag
     3121 acacgaacag caaagcgatg actaaaagat tgcgttgcga atccatcgtt agtgttctct
     3181 ggtatcaaat ggtccgggcg ggacgggatc gtcaccacca gggtgtaaag ggtggcattt
     3241 taatacgcgt ttcaccgtca accaactgcc ttttatcact ccaaacctgc gcaatgcctc
     3301 aattccgtag cttgaacagg ttggagtgaa acgacaatgc ggcccgagta gcggactaat
     3361 caggcgttga tagacccgaa tgagggctat caggacccgc gagccaggcg acagtggcgg
     3421 cgccataatt tttccaacgc ttccgagaga gcacggttat cgaggtcggc aacccctttt
     3481 ttcgccacca ccacgaaatc catagccggg agttcatgtt ggcgcagacg gaagctttca
     3541 cgcgtcagac gtttaatccg attgcgttca tgggcgcgtc gaacgttttt cttggcgact
     3601 gtaagaccga tacggggatg ccccagcgaa ttcaggcggc cgagaatggt aatttgcggc
     3661 gtgccagccc gttgtggctg ctggaagacg aatgtgaatt gactgggagt taacaagcgt
     3721 aactccctgg gaaatgcgag cttaaccact caggggttag ctttattact tagaaacggt
     3781 cagacgagcg cggcctttag cacgacgacg tgccagaacc tgacgaccat ttttagtagc
     3841 catacgagca cggaagccgt gagaacggtt gcgcttcagt acagacggtt gaaaagtgcg
     3901 tttcatggcg atttctacct aaacttgaat aaattcaatg gctttattgg atatccgccg
     3961 aaaaatgaaa cgatggacac cgaagccatg ggtgattaaa gaggccggat tgtaataatt
     4021 gtacactccg gagtcaattc tctttcctta tttaccgcgc ttttccgcac cttttcgcag
     4081 ggaaaatgta cgacctcaca ccagtggaaa ccagcatggc gcgccgggtg gaggattata
     4141 cgggctgatg ggtaaagcgc aaggatcgtc ctggatcttt attagatcga ttaagccaat
     4201 ttttgtctat ggtcattaaa ttttccaata tgcggcgtaa atcgtgcccg cctcgcggca
     4261 ggatcgttta cacttagcga gttctggaaa gtcctgtgga taaatcggga aaatctgtga
     4321 gaaacagaag atctcttgcg cagtttaggc tatgatccgc ggtcccgatc gttttgcagg
     4381 atcttgatcg ggcatataac cgcagacagc ggttcgtgcg tcaccctcaa gcagggtctt
     4441 ttcgacgtac gtcaacaatc atgaatgttt cagccttagt cattatcgac ttttgttcga
     4501 gtggagtccg ccgtgtcact ttcgctttgg cagcagtgtc ttgcccgatt gcaggatgag
     4561 ttaccagcca cagaattcag tatgtggata cgcccattgc aggcggaact gagcgataac
     4621 acgctggccc tgtacgcgcc aaaccgtttt gtcctcgatt gggtacggga caagtacctt
     4681 aataatatca atggactgct aaccagtttc tgcggagcgg atgccccaca gctgcgtttt
     4741 gaagtcggca ccaaaccggt gacgcaaacg ccacaagcgg cagtgacgag caacgtcgcg
     4801 gcccctgcac aggtggcgca aacgcagccg caacgtgctg cgccttctac gcgctcaggt
     4861 tgggataacg tcccggcccc ggcagaaccg acctatcgtt ctaacgtaaa cgtcaaacac
     4921 acgtttgata acttcgttga aggtaaatct aaccaactgg cgcgcgcggc ggctcgccag
     4981 gtggcggata accctggcgg tgcctataac ccgttgttcc tttatggcgg cacgggtctg
     5041 ggtaaaactc acctgctgca tgcggtgggt aacggcatta tggcgcgcaa gccgaatgcc
     5101 aaagtggttt atatgcactc cgagcgcttt gttcaggaca tggttaaagc cctgcaaaac
     5161 aacgcgatcg aagagtttaa acgctactac cgttccgtag atgcactgct gatcgacgat
     5221 attcagtttt ttgctaataa agaacgatct caggaagagt ttttccatac cttcaacgcc
     5281 ctgctggaag gtaatcaaca gatcattctc acctcggatc gctatccgaa agagatcaac
     5341 ggcgttgagg atcgtttgaa atcccgcttc ggttggggac tgactgtggc gatcgaaccg
     5401 ccagagctgg aaacccgtgt ggcgatcctg atgaaaaagg ccgacgaaaa cgacattcgt
     5461 ttgccgggcg aagtggcgtt ctttatcgcc aagcgtctac gatctaacgt acgtgagctg
     5521 gaaggggcgc tgaaccgcgt cattgccaat gccaacttta ccggacgggc gatcaccatc
     5581 gacttcgtgc gtgaggcgct gcgcgacttg ctggcattgc aggaaaaact ggtcactatc
     5641 gacaatattc agaagacggt ggcggagtac tacaagatca aagtcgcgga tctcctttcc
     5701 aagcgtcgat cccgctcggt ggcgcgtccg cgccagatgg cgatggcgct ggcgaaagag
     5761 ctgactaacc acagtctgcc ggagattggc gatgcgtttg gtggtcgtga ccacacgacg
     5821 gtgcttcatg cctgccggaa gatcgagcag ttgcgtgaag agagccacga tatcaaagaa
     5881 gatttttcaa atttaatcag aacattgtca tcgtaaacct atgaaattta ccgtagaacg
     5941 tgagcattta ttaaaaccgc tacaacaggt gagcggtccg ttaggtggtc gtcctacgct
     6001 accgattctc ggtaatctgc tgttacaggt tgctgacggt acgttgtcgc tgaccggtac
     6061 tgatctcgag atggaaatgg tggcacgcgt tgcgctggtt cagccgcacg aacctggtgc
     6121 gacgaccgtt ccggcgcgca aattctttga tatctgccgt ggtctgcctg aaggcgcgga
     6181 aattgccgtg cagctggaag gtgaacggat gctggtgcgc tccgggcgta gccgtttttc
     6241 gctgtctacc ctgccagcgg cggatttccc gaacctcgat gactggcaga gcgaagtcga
     6301 atttaccctg ccgcaggcga cgatgaagcg tctgattgaa gcgacccagt tttctatggc
     6361 gcatcaggac gttcgctatt acttaaacgg tatgctgttt gaaaccgaag gtgaagaact
     6421 gcgcaccgtg gcaaccgacg gccaccgtct ggcggtctgt tcaatgccaa ttggtcaatc
     6481 tttgccaagc cattcggtga tcgtaccgcg taaaggcgtg attgaactga tgcgtatgct
     6541 cgacggcggc gacaatccgc tgcgcgtaca gattggcagc aacaacattc gcgcccacgt
     6601 tggcgacttt atcttcacct ccaaactggt ggatggtcgc ttcccggatt atcgccgcgt
     6661 tctgccgaag aacccggaca aacatctgga agctggctgc gatctgctca agcaggcgtt
     6721 tgctcgcgcg gcgattctct ctaacgagaa attccgcggc gtacgtcttt atgtcagcga
     6781 aaaccagctg aaaatcaccg ccaacaaccc ggaacaggaa gaagcggaag agatcctcga
     6841 cgttacctat agcggtgcgg agatggaaat cggcttcaac gtcagctatg tgctggatgt
     6901 tctgaacgcg ctgaaatgcg aaaacgtccg catgatgctg accgattcgg tttccagcgt
     6961 gcagattgaa gatgccgcat cacagtcggc tgcctatgtt gtcatgccaa tgagactgta
     7021 atgtccctca cccgcttgtt gatccgcgat ttccgcaaca ttgaaaccgc ggatctcgct
     7081 ttatctcccg gctttaactt tctggtaggt gccaacggca gtggcaaaac cagcgtgctg
     7141 gaagccatct atacgctcgg ccatggtcgg gcgtttcgca gtttgcagat tggtcgcgtc
     7201 attcgccatg agcaggaggc atttgttctc catgggcgat tacagggcga agagcgcgag
     7261 acggcgattg gcttaaccaa ggacaaacag ggcgacagca aagtccgcat cgacggtact
     7321 gacgggcata aagtcgcgga actggcgcac ctgatgccaa tgcagctgat aacgccagaa
     7381 gggtttactt tactcaacgg cggccccaaa tacagaagag cattcctcga ctggggatgc
     7441 tttcacaacg aacccggatt tttcaccgcc tggagcaatc tcaagcgatt tctcaagcag
     7501 cgcaatgcgg cgctgcgcca ggtgacacgt tacgaacagc tacgcccgtg ggataaagag
     7561 ctgatcccgc tggcggagca aatcagcacc tggcgcgcgg agtatagcgc cggtatcgcg
     7621 gctgatatgg ccgatacctg taagcaattt ctccctgagt tttctctgac tttctctttc
     7681 cagcgcggct gggagaaaga gacagaatat gctgaggtgc tggaacgtaa ttttgaacgc
     7741 gatcgccagc taacctacac cgcgcacggc ccgcataaag cggacttacg cattcgcgcc
     7801 gacggtgctc cggtggaaga taccttatcg cgtgggcagc ttaagctgtt gatgtgcgcc
     7861 ttacgtctgg cgcaaggaga gttcctcacc cgtgaaagcg ggcggcggtg tctctacctg
     7921 atagatgatt ttgcctctga gcttgatgat gagcgtcgcg ggttgcttgc cagccgctta
     7981 aaagcgacgc aatcacaggt ctttgtcagc gcgatcagtg ctgaacacgt tatagacatg
     8041 tcggacgaaa attcgaagat gtttaccgtg gaaaagggta aaataacgga ttaacccaag
     8101 tataaatgag cgagaaacgt tgatgtcgaa ttcttatgac tcctccagta tcaaagtcct
     8161 gaaagggctg gatgcggtgc gtaagcgccc gggtatgtat atcggcgaca cggatgacgg
     8221 caccggtctg caccacatgg tattcgaggt ggtagataac gctatcgacg aagcgctcgc
     8281 gggtcactgt aaagaaatta tcgtcaccat tcacgccgat aactctgtct ctgtacagga
     8341 tgacgggcgc ggcattccga ccggtattca cccggaagag ggcgtatcgg cggcggaagt
     8401 gatcatgacc gttctgcacg caggcggtaa atttgacgat aactcctata aagtgtccgg
     8461 cggtctgcac ggcgttggtg tttcggtagt aaacgccctg tcgcaaaaac tggagctggt
     8521 tatccagcgc gagggtaaaa ttcaccgtca gatctacgaa cacggtgtac cgcaggcccc
     8581 gctggcggtt accggcgaga ctgaaaaaac cggcaccatg gtgcgtttct ggcctagcct
     8641 cgaaaccttc accaatgtga ccgagttcga atatgaaatt ctggcgaaac gtctgcgtga
     8701 gttgtcgttc ctcaactccg gcgtttccat tcgtctgcgc gacaagcgcg acggcaaaga
     8761 agaccacttc cactatgaag gcggcatcaa ggcgttcgtt gaatatctga acaagaacaa
     8821 aacgccgatc cacccgaata tcttctactt ctccaccgaa aaagacggta ttggcgtcga
     8881 agtggcgttg cagtggaacg atggcttcca ggaaaacatc tactgcttta ccaacaacat
     8941 tccgcagcgt gacggcggta ctcacctggc aggcttccgt gcggcgatga cccgtaccct
     9001 gaatgcctac atggacaaag aaggctacag caaaaaagcc aaagtcagcg ccaccggtga
     9061 cgatgcgcgt gaaggcctga ttgcggtcgt ttccgtgaaa gtgccggacc cgaaattctc
     9121 ctcccagacc aaagacaaac tggtttcttc tgaggtgaaa tcggcggttg aacagcagat
     9181 gaacgaactg ctggcagaat acctgctgga aaacccaacc gacgcgaaaa tcgtggttgg
     9241 caaaattatc gatgctgccc gtgcccgtga agcggcgcgt cgcgcgcgtg aaatgacccg
     9301 ccgtaaaggt gcgctcgact tagcgggcct gccgggcaaa ctggcagact gccaggaacg
     9361 cgatccggcg ctttccgaac tgtacctggt ggaaggggac tccgcgggcg gctctgcgaa
     9421 gcaggggcgt aaccgcaaga accaggcgat tctgccgctg aagggtaaaa tcctcaacgt
     9481 cgagaaagcg cgcttcgata agatgctctc ttctcaggaa gtggcgacgc ttatcaccgc
     9541 gcttggttgt ggtatcggtc gtgacgagta caacccggac aaactgcgtt atcacagcat
     9601 catcatcatg accgatgcgg acgtcgacgg ctcgcacatt cgtacgctgc tgttgacctt
     9661 cttctatcgt cagatgccgg aaatcgttga acgcggtcac gtctacatcg ctcagccgcc
     9721 gctgtacaaa gtgaagaaag gcaagcagga acagtacatt aaagacgacg aagcgatgga
     9781 tcagtaccag atctctatcg cgctggacgg cgcaacgctg cacaccaacg ccagtgcacc
     9841 ggcattggct ggcgaagcgt tagagaaact ggtatctgag tacaacgcga cgcagaaaat
     9901 gatcaatcgt atggagcgtc gttatccgaa agcaatgctg aaagagctta tctatcaacc
     9961 gacgttgacg gaagctgacc tctctgatga gcagaccgtt acccgctggg tgaacgcgct
    10021 ggtcagcgaa ctgaacgaca aagaacagca cggtagccag tggaagtttg atgtccacac
    10081 caatgccgag caaaacctgt tcgagccgat tgttcgcgtg cgtacccacg gtgtggatac
    10141 tgactatccg ctggatcacg agtttatcac cggtggcgaa tatcgtcgta tctgcacgct
    10201 gggtgagaaa ctgcgtggct tgctggaaga agatgcgttt atcgaacgtg gcgagcgtcg
    10261 tcagccggta gccagcttcg agcaggcgct ggactggctg gtgaaagagt cccgtcgcgg
    10321 cctctccatc cagcgttata aaggtctggg cgagatgaac ccggaacagc tgtgggaaac
    10381 caccatggac ccggaaagtc gtcgtatgct gcgcgttacc gttaaggatg cgattgctgc
    10441 tgaccagctg ttcaccacgc tgatgggcga cgccgttgaa ccgcgccgtg cgtttattga
    10501 agagaacgcc ctgaaagcgg cgaatatcga tatttaatgg cgttaaccat gcgagcgtgc
    10561 ctgatgcgct acgcttatca ggcctacgag aatcttgcaa tgtattgaat tttcatgatt
    10621 ttgtaggccg ggtaaggcgt tagcgccgca tctggcaaga agctataaga aaagggcgga
    10681 gatcatctcc gcccttttta tttctgcaat ccgcaattat catcccgcag atctatactt
    10741 tttgggtcaa cacgtaacgc tttacaggtg attatcatgg ggctttttga tgaagttgtc
    10801 ggtgcctttc tgaaaggcga tgcggggaaa tatcaggcta ttttaagttg ggttgaggag
    10861 cagggcggca ttcaggtgct gctggaaaaa ctgcaaagtg gcggcttagg ggccattctc
    10921 tcaacctggc tgagtaatca acagggcaat caatcggtta gtggcgagca actggaatcg
    10981 gcgctcggca caaatgcggt gtccgatctt ggacaaaaac ttggcgtgga taccagtaca
    11041 gcttccagtt tactggcaga acaattgccg aagattattg atgcgctctc accgcaaggt
    11101 gaagtgtctc cacaagccaa taacgatctg ctttccgcag gcatggaact gctgaaaggg
    11161 aaactcttcc gctaagcaaa tggggagcat gcaacccgcg ttgcatcccc cttttccctc
    11221 gggtgttgta ctgatttttg agcggaatcg cgttagcatg ggtcaggaac taatctacct
    11281 ggggaactca tggctatcaa actcattgct atcgatatgg atggcaccct tctgctgccc
    11341 gatcacacca tttcacccgc cgttaaaaat gcgattgccg cagctcgcgc ccgtggcgtg
    11401 aatgtcgtgc taacgacggg tcgcccgtat gcaggtgtgc acaactacct gaaagagctg
    11461 catatggaac agccgggcga ctactgcatt acttataacg gcgcgctggt acagaaggcc
    11521 gctgatggta gcaccgtggc gcaaactgct ctcagctatg acgactatcg tttcctggaa
    11581 aaactctctc gcgaagtcgg ttctcatttc cacgccctgg accgcaccac gctgtacacc
    11641 gccaaccgtg atatcagcta ctacacggtg catgaatcct tcgttgccac cattccgctg
    11701 gtgttctgcg aagcggagaa aatggacccc aatacccagt tcctgaaagt gatgatgatt
    11761 gatgaacccg ccatcctcga ccaggctatc gcgcgtattc cgcaggaagt gaaagagaaa
    11821 tataccgtgc tgaaaagtgc gccgtacttc ctcgaaatcc tcgataaacg cgttaacaaa
    11881 ggtacggggg tgaaatcact ggccgacgtg ttaggtatta aaccggaaga aatcatggcg
    11941 attggcgatc aggaaaacga tatcgcaatg attgaatatg caggcgtcgg tgtggcgatg
    12001 gataacgcta ttccttcggt gaaagaagtg gcgaactttg tcaccaaatc caaccttgaa
    12061 gatggcgtgg cgtttgctat tgagaagtat gtgctgaatt aatcagtgga cgggcaaaca
    12121 gcataaacgc gtttgcccgc ttactgatta ttggtttact gcatatacaa ccagaaattg
    12181 cagcggtgca gtaatgatgt ccagcgtaac agtcactggc agtaatgctg cggaaacacc
    12241 atccggaatg cctctgggtg aatagtattc ataaaatcgc actttaaacg gatgatagaa
    12301 cgccatcacc tttgagtaat ccatgttttt atttttctca tggattgtgc cttttaggtt
    12361 ttccagtgac ctggtacagt gttgaacacc acaggcaaaa ccataatgcg ttgccagtgc
    12421 cttttcttct tcgttattcc agtagtagta aagcgatatt gtgccggtaa atttcttttt
    12481 ccccgcattt aaaacaaatc ttgcgtcatc ggcaacctga atattgtgcc ggttaagtgc
    12541 tggatcattg agcatcttaa cgatgtcatc ggcaccatca gtgataaggt aatccagcga
    12601 ttcccctaca aaaacatagc cttttgttcc attactatct tttgcctgcg atagcccagt
    12661 gatggtgtct gactgcgatt gatactttcc tgaactaccc tttgcggtat gggaaaccag
    12721 agcatacgag atacagcctg aaagcatcag cgccagtggg aataaaccgg cagccttaaa
    12781 aaatccctta aaattcaact tcatgattaa tcctttaaac gagtagctaa gccgttgaaa
    12841 gcatactctt ctttccctcc tgtttagcgc ctgatatccc ttttcagatt tctgcccgac
    12901 gcatgtcatt tttttatgca ttgttctttt tgtgatctaa attgtagtac aacaatataa
    12961 gtttgtacta cattacgcac acggcaaacg cgaacgtcat cacgctggta ctacaaagtt
    13021 gccgcgttat gcatcgatcg gggtaaagta gagaagaaca tacagagcac aaggactctc
    13081 catgactctc aataaaaccg atcgcattgt cattacgctg ggtaaacaga tcgttcacgg
    13141 caaatacgta cctggctcgc cacttccggc tgaggcggag ctctgtgaag agtttgcaac
    13201 ctcgcgcaac atcatccgtg aggtgttccg ttcgctgatg gcgaagcggc tgattgaaat
    13261 gaaacgttat cgcggggcgt ttgtggcacc gcgtaaccag tggaattacc tcgacactga
    13321 cgtactgcaa tgggtgttgg aaaacgacta cgacccacgg cttatcagtg ccatgagcga
    13381 agtgcgaaat ctggtggaac cggcgattgc ccgttgggca gcagagcgcg cgacttccag
    13441 cgatctggcg cagattgaat cggcgctgaa cgagatgatt gccaacaatc aggaccgcga
    13501 agcgtttaac gaagcggata ttcgctacca cgaggcggtg ctgcagtcgg tacataaccc
    13561 ggtgttacag caacttagca ttgcgatcag ttcgttgcag cgggcggttt ttgaacgaac
    13621 ctggatgggc gatgaggcca acatgccgca aacgctccag gaacataagg cgctgttcga
    13681 tgcgatacga catcaggacg gcgatgcggc agagcaggcg gcgcttacca tgatcgccag
    13741 ctcgacacga aggttaaagg aaatcacatg acagctcgct acatcgcaat tgactgggga
    13801 tcgaccaatc tgcgtgcctg gctttatcag ggcgaccact ggctggacag caggcaatca
    13861 gaagcaggcg tcacgcgcct gaacggaaaa tctccggctg cggtgttagc agaagtcacg
    13921 accgactggc gtgaagagaa tacgccagtg gtgatggcag gaatggtcgg cagcaatgtc
    13981 ggctggaaag ttgcaccgta tttatctgtt cctgcccgtt tttcgtctat tggcgatcaa
    14041 ttaacgtcgg ttggcgacaa tatctggatt attcccggat tatgcgtctc tcatgacgat
    14101 aaccacaatg tgatgcgcgg cgaagaaaca caattgatcg gcgcgcgcac tctggctcct
    14161 tcctctcttt atgttatgcc cggtacgcat tgcaaatggg tgcaggccga tagccagcaa
    14221 atcaacgatt ttcgcaccgt gatgaccggt gaactacatc atttactgtt aaatcactca
    14281 ttgattggcg caggtttgcc gccgcaggaa aactctgccg atgccttcgc ggctggcctt
    14341 gagcgcggcc ttaatgcgcc cgccatattg ccgcagcttt ttgaagttcg cgcctcgcat
    14401 gtgctgggaa cacttccccg cgaacaggtc agcgaatttc tctctggttt gttgattggc
    14461 gcagaggttg ccagtatgcg cgactatgtg acccatcaac acgccatcac ccttgtcgcc
    14521 ggaacatcgc tgaccgcgcg ctaccagcaa gcctttcagg cgatgggttg cgacgtgacg
    14581 gcggtggcgg gcgacacggc atttcaggct ggtataagga gcatcgctca tgcagtggca
    14641 aactaaactt ccgctgatcg ccattttgcg cggcattaag cccgacgagg cgctggcgca
    14701 tgttggcgcg gtgattgacg ccgggttcga cgcggttgaa atcccgctga attccccaca
    14761 atgggagcaa agcattcccg ccatcgttga tgcgtatggc gacaaggcgt tgattggcgc
    14821 aggtacggta ctgaaacctg aacaggtcga tgcgctcgcc aggatgggct gtcagctcat
    14881 cgttacgccc aatatccata gtgaagtgat ccgccgtgcg gtgggctacg gcatgaccgt
    14941 ctgccccggc tgcgcgacgg cgaccgaagc ctttaccgcg ctcgaagcgg gcgcgcaggc
    15001 gctgaaaata tttccgtcat cggcttttgg tccgcaatac atcaaagcgt taaaagcggt
    15061 attgccatcg gacatcgcag tctttgccgt tggcggcgtg acgccagaaa acctggcgca
    15121 gtggatagac gcaggttgtg ctggggcggg cttaggcagc gatctctatc gcgccgggca
    15181 gtccgtagaa cgcaccgcgc agcaggcagc agcatttgtt aaggcgtatc gagaggcagt
    15241 gcaatgaaaa tcaccaaaat taccacgtat cgtttacctc cccgctggat gttcctgaaa
    15301 attgaaaccg atgaaggcgt ggtcggttgg ggcgagcccg tgattgaagg ccgcgcccgt
    15361 acggtggaag cggcagttca cgagctgggt gactatttga ttggtcagga tccttcgcgc
    15421 atcaatgact tatggcaagt gatgtatcgc gccggatttt atcgtggcgg tccaatcctg
    15481 atgagcgcca ttgccgggat cgaccaggcg ttatgggata tcaaaggcaa agtgctgaat
    15541 gcgccggtct ggcaactgat gggcggcctg gttcgcgaca aaattaaagc ctacagttgg
    15601 gtcggcggcg atcgtccggc ggatgttatc gacggcatta aaaccctgcg cgaaatcggc
    15661 ttcgatacct tcaaactgaa cggttgtgaa gaactggggc taattgataa ctcccgcgcg
    15721 gtagatgcgg cagtcaacac cgtggcacaa attcgtgaag cttttggcaa tcagattgag
    15781 tttggtcttg atttccacgg tcgcgtcagc gcgccaatgg cgaaagtgct gattaaagaa
    15841 ctggagccgt atcgcccgct gtttattgaa gagccggtgc tggcggaaca agccgaatac
    15901 tacccgaaac tggcggcaca aacgcatatt ccactggcgg cgggtgagcg catgttctca
    15961 cgtttcgatt ttaaacgcgt gctggaggca ggcggtattt cgattctgca accggatctc
    16021 tcccatgcag gcggtattac cgaatgctac aaaattgctg gaatggcaga agcctatgat
    16081 gtgacccttg cgccgcactg tccgctcgga ccgattgcac tggcggcttg cctgcatatc
    16141 gactttgttt cctataacgc ggtacttcag gaacaaagta tgggcattca ttacaacaaa
    16201 ggcgcggagt tactcgactt tgtgaaaaac aaagaagact tcagtatggt tggcggcttc
    16261 tttaaaccgt taacgaaacc gggcttaggt gtggaaatcg acgaagctaa agttattgag
    16321 ttcagtaaaa atgccccgga ctggcgtaat ccgctctggc gtcatgaaga taacagcgta
    16381 gcagagtggt aattcctgcc acgtaagccc ctcatcgggc actaaaacag caatacaaaa
    16441 atataaccct ctgtaaatta cagggcatgg tgagcggctt cgctatgccc aaaatctgga
    16501 gaaagctcgc tatggatatt cccgttaatg cagcaaagcc ggggcgtcgg cgttatctga
    16561 cgctggtgat gatctttatt acggtggtca tttgttatgt tgaccgcgct aacctggccg
    16621 tggcttccgc ccatattcag gaagagttcg gcattacgaa agcgcaaatg ggctatgtat
    16681 tttcggcctt cgcctggctt tatacgctat gccagatccc cggcggttgg tttttagatc
    16741 gcgtaggttc tcgcgtgact tattttattg cgatatttgg ctggtcagtg gcgactttat
    16801 tccagggctt tgcctcggga ttaatgtcat taattggtct gcgcgcgata accggtattt
    16861 tcgaagcgcc cgctttcccg accaataacc ggatggtgac cagctggttc ccggaacatg
    16921 aacgcgcttc cgccgttggt ttttatacgt ctggtcagtt tgtcggtctg gcgtttttga
    16981 ctccgctgct gatctggatt caggagatgt tgagctggca ctgggtgttc attgtcactg
    17041 gtggtatcgg cattatctgg tcgctgattt ggtttaaggt ttatcagccg ccgcgcctga
    17101 ccaaaggtat cagcaaagct gaactggatt acattcgtga tggcggcggt ctggtggatg
    17161 gtgatgcgcc ggtgaagaaa gaggcgcgtc agccgttaac agccaaagac tggaaactgg
    17221 tgttccatcg taaactgatc ggcgtttatc ttgggcaatt tgcggtggct tctacactgt
    17281 ggtttttctt aacctggttc ccgaactatt taacccagga aaaaggaatc acggcgctga
    17341 aagcaggctt tatgaccacg gtgccattcc tcgcggcgtt tgtcggcgtc ctgctctctg
    17401 gctgggtagc ggatctgctg gtacgtaagg gcttttcact gggctttgcg cgtaaaacgc
    17461 cgattatctg cggcttgctg atctccacct gcattatggg cgctaactac actaacgatc
    17521 cgatgatgat tatgtgcctg atggcgctgg cattcttcgg taacggtttt gcttcgatta
    17581 cctggtcgct ggtttcttct ctggcaccga tgcgcctgat tggtttaacc ggcggcgtgt
    17641 ttaacttcgc cggtggtctg ggcggcatca ccgttccgct ggtggtgggg tacctggcgc
    17701 agggttacgg tttcgcacct gcactggttt atatctccgc cgtcgcgttg attggcgcgc
    17761 tctcttatat cctgctggtg ggcgatgtga agcgcgttgg ctaatccttc aactgtggaa
    17821 tatgtgccac accactgcgc ataatccact ttcttaaagc cggtgcggtc aggcatcggc
    17881 tttttactat cttgccgaag agttttaaac gcagtttgcg ggtggcgcgc cagtaagcgg
    17941 tattgagctt ctctggctgc ttcagtaaca ccgcacgcag aatgtctgcg ctatccagcg
    18001 catagctaat cccctccagc gaactggcgc tgataaatcc tgccgcttcg ccaatcagaa
    18061 aggcgttgtc cttaccgcag acaaaatcct gccagcgtga gggaaacagc accgtgcatt
    18121 tttcgctttt caccgcctta ccaaactgga actgaaaggc gctcattttc tctttcagcg
    18181 tcgtgaaacg cgtctgaccg tctttcattg gataagcacc gccaaagata aaataaccgt
    18241 ctttgctgat actccatgaa taacagttag ttatcgaatt atcaaagatg caggagtaga
    18301 acggcaccgg atgtttctct gcgaaccact gctggatagc gacatattta cggatttgat
    18361 gatccgggta gagatgtcgc cgcaccatcg agttggcacc atcggcaccg accagatagc
    18421 gggcggtaat atgctgctcc cagccgtctg cacgaaaaat gacatgccat ttatcatcct
    18481 cacgccagat ttttcggcac aggctatcgt gataaacctc aacgctggtg gggatcagcg
    18541 atttcatcca caagtcgaaa gcatggcgat taatattgat atagcttcgc tggtagttac
    18601 gcgtgagcga tgcggcgacg tcgacagttt tgacgctgaa aatctgtgga ttggcgatca
    18661 catcgacagg aagcgtcagt ccatcgcgaa taaaagatcg ctgcgcgtcc ggtgccagca
    18721 gaccgccgca aggtttgctg aaaccttcag taccacactg gtgcttttta tccagcgcga
    18781 tcacctgcat tttgccggct aactttcgtg ccaacgccga tcctgccggg ccgaggccaa
    18841 taatcgccac gtcgaaatgt tccatctttt cactcacatt catcacgatg gcaaaagact
    18901 attgtctgaa agtgaagtcc ttgtgaagta tgatggcggg aaccgttttg tatcaggacc
    18961 gcgccatgaa acagataacc tttgctcccc gtaatcacct gctcaccaat accaatacct
    19021 ggacgcccga cagccagtgg ctggtatttg acgtgcgtcc ttctggggcg tcgtttaccg
    19081 gcgagaccat tgagcgtgtg aatatccata caggcgaggt cgaggttatc tatcgcgcgt
    19141 cacagggcgc acacgtcggc gtggtgaccg ttcatccgaa gtcagagaaa tatgtcttta
    19201 ttcacggccc tgaaaatcct gatgaaacat ggcattacga cttccatcat cgacgcggag
    19261 tcattgctga aggcggcaag gtgagcaatc tcgatgcaat ggatattacc gcgccgtata
    19321 cgccaggagc gctgcgcggc ggcagccatg tgcatgtctt tagcccgaac ggtgaaaggg
    19381 tgagctttac ctataacgac catgtaatgc atgaactcga tccggcgctg gatttgcgaa
    19441 acgtcggtgt tgctgcgccg tttggcccgg tcaacgtaca aaagcagcat ccgcgtgaat
    19501 acagcggtag ccactggtgc gtgctggtga gtaaaaccac gcccacgccg cagcctggca
    19561 gcgatgaaat caatcgtgct tatgaagaag gatgggtagg aaatcacgcg ctggcgttta
    19621 ttggcgacac actttcgcca aagggcgaga aagtgccgga gctgtttatc gttgagttac
    19681 cgcaagatga agctggctgg aaagcggcag gtgatgcgcc gttaagtgga acggaaacca
    19741 ccctgcccgc gccaccgcgc ggcgtcgtgc agcgacgttt aacctttacc caccatcggg
    19801 cttatccggg gttagtcaac gtcccgcgcc actgggtgcg ctgtaatccg cagggtacgc
    19861 aaatcgcgtt tttaatgcgt gatgataacg gcattgtgca actgtggctt atctcgccac
    19921 agggcggcga gccgcgccag ttaacccata acaaaacgga tattcagtct gcatttaact
    19981 ggcatccgtc aggagaatgg ttgggctttg tgctggataa tcgaattgct tgtgcccatg
    20041 cgcaaagcgg cgaggttgag tatttaaccg aaaaccacgc caatccacct tctgcggacg
    20101 ccgtggtctt ctcgccggat ggtcagtggc tggcgtggat ggaaggcggc cagctgtgga
    20161 tcaccgaaac tgatcgctaa atcagttatc aggcattctg gccggtggaa taaccgcatt
    20221 ggtggcttgc gcgttggctt cgcttttttc aacgcgcgag cgcaccgagc tgtctttgcg
    20281 gaacacatcc cacggcagta aaagcgtgtc catcacagcg gtaaacggca tatcgagaat
    20341 cgccagcgac ttagtgcccc agttggtttc atcgtcgcca atcatggttg cgctggcgcg
    20401 ggtgccggga tacgttcctt ctttccctcc ggtatgggac atcacgctgg agcatcctcc
    20461 gagtaatgtc attccgctgc atatcatgaa cgctaacaac acatttctta tcataataaa
    20521 ttcatctgtt gatcgtgggt gttggcctga tgagttatag cgatcccttg ctgaaaataa
    20581 catcatcatt acgtcgcact gtggcggcta tcgcacttta acgtttcgtg ctgccccctc
    20641 agtctatgca atagaccata aactgcaaaa aaaagtccgc tgataaggct tgaaaagttc
    20701 atttccagac ccatttttac atcgtagccg atgaggacgc gcctgatggg tgttctggct
    20761 acctgacctg tccattgtgg aaggtcttac attctcgctg atttcaggag ctattgatta
    20821 tgcgtaactt tgatttatcc ccgctttacc gttctgctat tggatttgac cgtttgttta
    20881 accacttaga aaacaaccag agccagagta atggcggcta ccctccgtat aacgttgaac
    20941 tggtagacga aaaccattac cgcattgcta tcgctgtggc tggttttgct gagagcgaac
    21001 tggaaattac cgcccaggat aatctgctgg tggtgaaagg tgctcacgcc gacgaacaaa
    21061 aagagcgcac ctatctgtac cagggcatcg ctgaacgcaa ctttgaacgc aaattccagt
    21121 tagctgagaa cattcatgtt cgtggcgcta acctggtaaa tggtttgctg tatatcgatc
    21181 tcgaacgcgt gattccggaa gcgaaaaaac cgcgccgtat cgaaatcaac taattcccta
    21241 aggccgcctg gcgcggcctg acatctccat gctcgccgtc agggagcata tgcgaatctt
    21301 cggatttgca ggtacttact cgcttcttag aaggagaaat gactatgcgt aacttcgatt
    21361 tatccccact gatgcgtcaa tggatcggtt ttgacaaact ggccaacgca ctgcaaaacg
    21421 ccggtgaaag ccagagcttc ccgccgtaca acattgagaa aagcgacgat aaccactacc
    21481 gcattaccct tgcgctggca ggtttccgtc aggaagattt agagattcaa ctggaaggta
    21541 cgcgcctgag cgtaaaaggc acgccggagc agccaaaaga agagaaaaaa tggctgcatc
    21601 aagggcttat gaatcagcca tttagcctga gctttacgct ggctgaaaat atggaagtct
    21661 ctggcgcaac cttcgtaaac ggtttactgc atattgattt aattcgtaat gagcctgaac
    21721 ccatcgcagc gcagcgtatc gctatcagcg aacgtcccgc gttaaatagc taactagcta
    21781 ttctctttgc cccgccattc tggtggggct ttttttgtct tacttactca acaatgcgtt
    21841 aaatttcgac tgtttaagat atttcggcac gttctttccc gtgataatgt gcgctataag
    21901 gcaaatctgc ttcaccgatt cagccataat ccgtagcaat taaaaatgtt atctcaagag
    21961 aaatggacga tgagtgatat agcattaacg gtcagtattc tggctttggt ggcagtcgtc
    22021 ggtttgttta tcggcaatgt caaatttcgc ggcataggat taggtattgg cggcgtgctg
    22081 tttggtggga tcatcgtcgg ccattttgtt tctcaggcgg ggatgacatt aagtagcgat
    22141 atgctgcatg ttattcagga atttggcctg atcctgttcg tttatactat cgggattcag
    22201 gtagggccgg gcttctttgc ctcattgcgc gtctccggat tacgcctcaa cctgtttgct
    22261 gttctgatcg tcatcatcgg tggtctggtt accgccatcc tgcataaact gtttgatatt
    22321 ccactgccgg tagtgctggg gattttctcc ggtgcggtta ccaatacgcc agcgctgggg
    22381 gcagggcagc agattttgcg cgacctgggt acaccaatgg aaatggtcga tcagatgggg
    22441 atgagttacg cgatggcgta tccattcggc atttgcggga ttttgttcac catgtggatg
    22501 ttgcgggtta ttttccgcgt caatgtcgag acagaagctc agcagcacga gtcttcacgc
    22561 accaatggcg gcgcgctgat caagactatc aatattcgcg ttgagaaccc taacctgcat
    22621 gatttagcca ttaaagatgt accgattctc aacggcgaca aaattatctg ctcgcgtctg
    22681 aaacgcgaag aaaccctaaa agttccttcg ccagatacca ttattcaact gggcgatttg
    22741 ctgcatctgg tgggtcagcc agcggattta cataatgcgc aactggtgat tggtcaggag
    22801 gtcgatactt cgctgtccac gaaaggcact gatttgcgcg tcgagcgtgt ggtggtcacc
    22861 aatgaaaacg tgctcggaaa acgtattcgc gacctgcact ttaaagaacg ctatgacgtt
    22921 gttatctcgc gcctgaaccg tgccggggtc gaactggtcg ccagtggcga tatcagcctg
    22981 cagttcggcg atatcctcaa cctggtgggg cgtccgtccg caattgatgc cgttgccaat
    23041 gtgctgggga atgcgcagca aaaactgcaa caggttcaga tgctgccagt gtttattggc
    23101 atcgggcttg gcgtattgtt aggttctatt cccgtctttg tgccaggatt cccggccgcg
    23161 ttgaaactgg ggctggcggg cggtccgctg attatggcgt tgatcctcgg gcgtatcggc
    23221 agtatcggca agctgtactg gtttatgccg ccaagtgcca acctcgcgct gcgggagctg
    23281 gggatcgtgc tgttcctctc ggtcgttggt ctgaaatctg gtggggattt tgtaaatacc
    23341 ctggtcaatg gcgaagggct aagctggatt ggttatggtg ccctgatcac cgccgttccg
    23401 ctgattactg ttggcattct ggcgcggatg ttagccaaaa tgaattacct gaccatgtgc
    23461 gggatgctgg caggttccat gaccgatcct ccggcgctgg cgtttgctaa taatcttcat
    23521 ccaaccagcg gtgcggcggc gctctcttac gccactgtct atccgttagt gatgttcctg
    23581 cgcattatca ccccccaatt actggcggtg ctcttctgga gtatcggtta actcttttcc
    23641 gggtggagtc gccgtaaatg gtactccacc tggtattcgc tggcatttct gaacatcact
    23701 gaataattca aaaactcccc gctctcgcta tatgacaacg aggtgatccg cagcagtggc
    23761 gtgtgttccg ctaccttcat ttgccgcgcc agcagtctat cggcgagcac tggcgtcagg
    23821 ctttcataat taccgccaat caaaatcccg cactcttgtt caatatattc aaacttcgac
    23881 ccttccagat gttgcagcga aagattacgg aacagtttta ccggcatata gctgtcttcc
    23941 agcatcagcg gtttcccttc cacaaaacga acgcgacggg agaagtagat ctgctcgttg
    24001 atttgaatcc gtaactggct ggcaatggcc ggaggcgcag gcatgatttc aaaaatcagc
    24061 acctggctgg tgaccgtttt tccctgccgt tttaacacct ccaccagccc ggtcaggctg
    24121 gcggtttgat gcagtacatc tttgcgcacc aggtaagtgc cgctgccgtg gcggcggacc
    24181 accaatcccc acgctaccag caggtcaatc gctttacgga tggtcatcag ggatactgca
    24241 aattcttccg ccaacttttt ttcaccggga agaaggctgt ttagcgtgaa atccgcggaa
    24301 ttaagtcgaa ttcttaaccg ctccgcaatg cttttgtaga tcatctctag atctcttttt
    24361 tgcctgcaag cccgggtaat gcgcccacaa gttctgctta atcgattgaa aaaacatctt
    24421 ttttttaaag atgtgttcga tgctgtgtac cagtgctcac agatgtctac tttttcgcga
    24481 aaacgtagat ctctaccgcc caacgaaaag catgaaagcg atcacgaatc ccattgggtc
    24541 gtcatgttct gccgatcgca tcttcctatc ctcgctccag gcctgccgca taaccaatca
    24601 ggcttcctac ttacagaatt gagaaaagag gatgtggaaa tgctcagtca aattcaacgc
    24661 tttggcggcg cgatgttcac gccagtgctg ctgtttccct tcgccgggat tgtggtgggt
    24721 cttgccatct tgctgcaaaa cccgatgttt gtcggggaat cactgaccga tccgaacagt
    24781 ttattcgcgc aaatcgtaca cattattgaa gagggcggtt ggacggtatt ccgtaatatg
    24841 ccgctgattt ttgctgtcgg tttacccatt ggccttgcta agcaagcgca ggggcgtgct
    24901 tgtctggcgg tgatggtgag tttcctgacc tggaactatt tcatcaacgc gatgggaatg
    24961 acctggggaa gctacttcgg cgtcgatttc actcaggacg cggtggcagg tagcggtctg
    25021 acaatgatgg ccgggattaa aaccctcgat accagcatta tcggcgcaat tatcatttcc
    25081 ggcattgtga cggcgctgca taaccgtctg ttcgataaaa aactgccggt ttttctcggc
    25141 attttccagg ggacgtctta tgtggtgatt atcgccttcc tggtgatgat cccctgtgcc
    25201 tggctgacat tgctcggctg gccaaaagta caaatgggga ttgaatctct gtaagcgttc
    25261 ctgcgttcgg cgggtgcgct tggggtgtgg gtttacacct tcctcgaacg tattctgatc
    25321 ccaaccggtt tacaccactt catctacgga cagtttatct ttggtccggc agctgttgaa
    25381 ggcggcattc agatgtactg ggcgcagcat ctgcaagagt tcagtctgag cgccgagccg
    25441 ctgaaatcgt tgttcccgga aggcggtttt gccctgcacg gtaactcaaa aatctttggt
    25501 gccgtgggca tttctttagc gatgtacttc actgccgcac cggaaaatcg ggtaaaagtg
    25561 gcgggcttgc tgattcccgc aaccttaacc gccatgctgg tgggaattac cgaaccgctg
    25621 gaatttacct tcctgttcat ttcaccgttg ctgtttgcgg tacacgctgt gcttgcggcc
    25681 tcaatgtcga ccgtgatgta tctctttggt gtggtgggca acatgggcgg aggtctgatt
    25741 gaccaggttt taccgcaaaa ctggatcccg atgttcagca accacgcgga tatgatgctg
    25801 acccaaatcg ccattgggtt gtgctttacc ctgctgtact tcgtggtttt ccgcaccctg
    25861 attctgcagt tcaacatgtg cacgccggga cgtgaagatg cggaagtgaa actctactca
    25921 aaagccgaat acaaagcctc gcgaggccaa accaccgcgg cagagccaaa aaaagagctg
    25981 gatcaggctg ccggtatcct gcaagccctg ggcggggtcg gcaatatctc cagcattaac
    26041 aattgtgcga cgcgtctacg cattgcactg catgacatgt cacaaacgct ggatgacgaa
    26101 gtctttaaaa agctgggagc gcacggcgtc ttccgtagtg gcgatgccat tcaggtgatc
    26161 attggtctgc atgtatccca gctgcgtgaa cagctcgata gcttaattaa ttctcatcaa
    26221 tcagcagaaa atgttgccat tacggaggca gtataatgac caaattctca gtggttgtcg
    26281 caggcggtgg aagcaccttt acgccaggca tcgtgttgat gctcctggtg aatcaggacc
    26341 gtttcccgct tcgtgcactg aaattttatg ataacgatgg tgcgcggcag gaagtgattg
    26401 ccgaagcctg taaagtcatc cttaaagaga aagcgccgga cattgcgttt agttacacca
    26461 ccgatcctga agtggcattc agcgacgttg attttgtcat ggcgcacatc cgcgtcggca
    26521 agtacccgat gcgcgaactg gatgaaaaaa tcccactgcg ccacggtgtt gttggtcagg
    26581 aaacttgcgg acccggcgga atagcgtacg gcatgcgttc cattggcggc gtcctggaac
    26641 tggtggatta tatggaaaaa tattcaccaa atgcctggat gctcaactac tccaacccgg
    26701 cagccattgt cgcagaagcc acgcgtcgtc tgcgcccgaa tgcgaaaatc ctcaacatct
    26761 gtgacatgcc aatcggtatt gaaagccgga tggcgcaaat tgttgggctg caagatcgca
    26821 aacagatgcg cgtgcgctac tacggcctga accactggtg gtcggcgata tcccgcagtt
    26881 tcagaaaggg ttaatgagtc agcaagtggc ggtggaaaaa ctggtcgtgg acgcctggga
    26941 acagcgttca tatcagcacc tgtggcaggc gattacgttg tcgaaaacgg taccgagcgc
    27001 ctcggtcgcc aaagctattc tggatgagtt gctggaggcc aacaaagcgt actggccaga
    27061 gttacgttaa ttgtgtggac tggcatccgt tgggtgctgg tccgtattct ttagcacggc
    27121 tgacggcgca taaccaaatt tatgtttaaa ggctttgctg aaatgaaagg agtcgaagaa
    27181 gttgagcgta tcagcgactt cgccaaccga gtttccttgt tgctttaata gcgatagcgc
    27241 cagatccaga cgcgcatcga ggtaatattc tttcggcgtc ttgccggtat agcgtaaaaa
    27301 cagacgacgc aaccaggctt cgctacaggg gatcgtggca gccatatcag ctacgctcca
    27361 gcgttgttgc agactggcat gtaaagtggc aattaatttt tcaatttgcc gccgttgtgg
    27421 atctttttta ccatctgcgt acataagaca aatccactga taaataattt tcgtcagaaa
    27481 agcgactgcc agattatttt ttattgcttc tggtgaagtt attaactcag caacttcggt
    27541 gagttcctga ttataaattt cgccattata aataacgctt tgctgaccaa cgggaatatc
    27601 catcatactg gtgggggtaa attccatcca gtactgttcc cagactaaac cttcacagtg
    27661 ataagagtga atatccattg gctttaagaa tataatacag ttgccatgca gtgttatttg
    27721 ttcgccattc tttaaaagaa ttttcccaca tccttgtacg gtatacaccg caacccatga
    27781 gttggcgata agcggtttct tcttgtcccg cggccagatc acccggtaat gctcgtcggc
    27841 gtaggtatgc cagagcgaga tcagagaaat tccgctggag atcacatttt cgttaatcaa
    27901 taagggaata ttgtacggag tttcgttttt tacatctgaa ctttgcaatt ttccattcat
    27961 tttaatatcc ctccggcgaa ttatgccaca acctgaactt aacagaaaac aatccgttca
    28021 gggatacgac gtttgtattt taagaatctg actgcctgac ccgacgcatt ttacctctcc
    28081 ctatattcat gcgtccggga cataaacata aataagggct atgagatgaa ttcgttacaa
    28141 atcttgagtt ttgtcggttt tacgctgctg gtggcggtga tcacctggtg gaaggtccgc
    28201 aaaacagata ccggatcgca acaaggctat tttcttgccg gacgttcact aaaagcgccg
    28261 gttattgccg cttcgttaat gctaaccaac ctttccacgg aacaactggt cggcctttcc
    28321 gggcaggcct acaaaagcgg catgtcggtg atgggctggg aagtgacttc agcggtgacg
    28381 ctgatcttcc tcgcgctaat ctttttaccg cgctatctga agcgcggcat tgccaccatc
    28441 cccgattttc tggaggaacg ttatgataaa acgacgcgta ttatcatcga cttctgcttc
    28501 ctcattgcca ccggcgtctg ctttctgccg attgttctct actccggcgc gttggcgctc
    28561 aacagcctgt ttcacgtcgg ggaatcgcta cagatttctc acggtgcggc tatctggcta
    28621 ctggtaattt tgcttggtct ggcgggaatt ttgtatgcgg tgatcggcgg actgcgcgca
    28681 atggcagtgg cggactccat caacggtatt gggctggtta tcggcgggtt gatggtgccg
    28741 gtatttggcc tgatcgcgat gggcaagggc agctttatgc agggcattga gcaactcacc
    28801 accgttcacg ccgagaaatt aaactcaatc ggtggcccga ccgatccctt gccgattggc
    28861 gcggcattta ccggtttgat tctggtgaac accttttact ggtgtacaaa tcagggcatc
    28921 gtgcaacgca cgctggcgtc aaaaagcctg gcggaagggc aaaagggggc gctgttaacg
    28981 gcggtgctga aaatgctcga cccgctggta ctggtgctgc cagggttgat tgcgtttcat
    29041 ctgtatcagg atttaccgaa agccgacatg gcctacccga cgctggtcaa taacgttctg
    29101 ccagtgccaa tggtgggttt cttcggcgcg gtgttatttg gtgcggtgat cagtaccttc
    29161 aacggctttc tgaatagcgc cagtacgtta ttcagtatgg gtatttaccg tcgcatcatt
    29221 aaccagaatg ccgagccgca gcagctggtc accgtcgggc gcaaatttgg tttctttatc
    29281 gctatcgttt cggtgctggt cgcgccgtgg atcgccaacg cgccgcaggg gctgtatagc
    29341 tggatgaaac agctcaacgg catttacaac gtgccgctgg ttaccatcat cattatgggc
    29401 tttttcttcc cgcgcatccc ggcgctggcg gcaaaagtgg cgatggggat tggcataatc
    29461 agctacatca ccatcaacta tctggtgaag ttcgacttcc atttcctcta tgtgctggcc
    29521 tgtacgttct gcatcaacgt ggtcgtgatg ctggtgatcg gttttatcaa accgcgcgcc
    29581 acgccgttca ccttcaaaga tgcgtttgcg gtggacatga aaccgtggag aaacgtcaag
    29641 atcgcgtcaa ttggcatcct gttcgcgatg attggcgtct atgccgggct ggctgaattc
    29701 ggcggctacg gtacgcgctg gttagcgatg atcagttatt tcattgccgc cgtagtgatt
    29761 gtctacctga tttttgacag ctggcggcat cgtcacgacc cagccgtaac ctttactccc
    29821 gacgcgaagg atagcctatg aaacgcccca attttctgtt catcatgacc gatacccagg
    29881 ccaccaatat ggtcggttgc tatagcggta aaccgctgaa tacgcaaaat attgatagtc
    29941 tggcggcgga aggtattcgc tttaattccg cctacacctg ttcaccggtt tgtacgcctg
    30001 cacgcgccgg actatttacc ggtatctacg ctaaccagtc cggcccgtgg accaacaacg
    30061 tcgcgccagg caaaaacatc tccactatgg ggcgctactt taaggatgcc ggctatcaca
    30121 cctgttacat cggcaaatgg catctcgacg gtcatgacta tttcggcact ggcgagtgtc
    30181 cgccggagtg ggacgctgat tactggttcg atggggcgaa ctaccttagc gaactgacgg
    30241 aaaaagagat cagcctgtgg cgcaatggcc taaacagcgt tgaggattta caggcgaacc
    30301 atatcgacga aaccttcacc tgggcgcacc gcatcagcaa tcgggcggta gattttctgc
    30361 aacagcccgc gcgcgccgag gaacccttcc tgatggtggt ttcgtatgat gagccgcatc
    30421 acccgttcac ctgtccggtg gagtatttag agaaatacgc tgatttttac tacgatctgg
    30481 gcgagaaagc tcaggatgac ctggcgaaca aaccggaaca tcaccgctta tgggcgcagg
    30541 cgatgccatc gccagtcggt gatgacgggc tttatcacca tccgctctat tttgcctgta
    30601 atgactttgt tgatgaccaa atcggacggg tcatcaatgc cttaacgcca gagcaacgtg
    30661 aaaatacgtg ggtcatttat acttccgatc acggcgaaat gatgggcgca cataagctga
    30721 tcagtaaagg agcggcgatg tatgacgaca tcacccgtat tccgctgatc atccgttcgc
    30781 cgcaagggga gcggcgacag gtcgatacgc cagtcagtca tatcgattta ctgccgacaa
    30841 tgatggcgct ggcagatatt gaaaaaccag agattctgcc gggggaaaat atccttgccg
    30901 tgaaagagcc acgcggtgta atggtggaat ttaaccgcta cgagattgag catgacagct
    30961 ttggcggttt tattccggtg cgttgctggg tgacggatga ctttaaactg gtactcaacc
    31021 tcttcaccag tgatgaactt tacgatcgcc gtaatgaccc aaatgaaatg cataacctga
    31081 tcgatgatat ccgttttgca gacgttcgca gcaaaatgca tgacgcctta ttggattaca
    31141 tggacaaaat tcgcgatccg ttccgcagtt accaatggag cctgcgtccg tggcgtaaag
    31201 atgcactgcc gcgctggatg ggggcatttc gtccacgccc acaagatggc tattcgccgg
    31261 tggtacgtga ctatgacacc ggcctaccga cgcaaggagt gaaagtggaa gagaaaaaac
    31321 agaagttctg acgataatgc ccgctgatga acacccggcg ggcattattc aggctgctta
    31381 aaccagtgaa ccgccggtca ggaaggaaaa aatcaggaat atcagcaaaa tgctgcacac
    31441 gatgccgata ccaaaaagca aggtatggaa agccagcgtc ccgccgccaa aaaccagcgc
    31501 gccgcgaatg ccccagcgat tttccttacg cataccgcca ataaatgcgc aaagtagcgc
    31561 agcaatggcc agtgcgatac cgctttgatc gaggatttta tcgacatcta cgtcctcttt
    31621 tttctccacg gtggtaattt tttcgccttt tattccggcg agaagacctc ttttaatttc
    31681 agccgttttg tccgccacga tgctttcaaa cgttggcggt ggtgcggaaa aagggccgaa
    31741 tgaaaagtgg ataatcccca tcattaaagc gatggcaccg aacagcatgc ccaatgaact
    31801 gattttattt tgagctatga tacccatctg acctccttgt agcggatggc gaaagcatat
    31861 caggaaacgg gcggacgaaa ttcttaaaaa aaaaccgcta aaggtagagc gttagtgaaa
    31921 ttgcaaagga gcaacaatga aaatttcccg ccttggagaa gcgcccgatt accgcttctc
    31981 gctggctaat gagcgtactt ttctggcgtg gatccgtacc gcactaggat ttctggcggc
    32041 gggggttggg cttgatcaac ttgcgccaga tttcgccacg cccgtcattc gtgaactgct
    32101 ggcgttgttg ttgtgtctgt tttccggcgg gttagcgatg tatggctacc tgcgctggtt
    32161 acgcaatgaa aaagcgatgc gtctgaaaga ggacctgccc tacaccaaca gcttattaat
    32221 cattagctta atattgatgg tcgtggcggt gatcgttatg ggactggtgt tgtatgccgg
    32281 atagccgcaa agccagacgc attgccgacc caggactgca gccggagcgc acatcactgg
    32341 cgtggtttcg taccatgctg ggctacggcg cgttgatggc gttggctatc aaacacaact
    32401 ggcaccaggc gggcatgtta ttctggattt cgattggcat tctcgccatc gtggcgctga
    32461 tcctctggca ctacacccgt aatcgcaatt taatggatgt cacgaatagc gatttttccc
    32521 aatttcacgt agtccgtgac aaatttttga tctccctcgc ggtgttatct ctcgcaatac
    32581 tgtttgctgt aacgcatata catcaactta tcgtatttat tgagagagtc gcatgacagg
    32641 aagtcaggtc attgatgctg aagaggacag gcataaactg gttgtagagt acaaggatgc
    32701 tctgcaacct gccgattttt atcacaattt caaacagcgg ggcatccgcg ccgtacagct
    32761 cattccctat cttgaatttg atgaccgggg cgatctgacg gccgcttcgg tgacagcaga
    32821 gctgtggggg aaatttttaa ttgccctgtt cgagtgttgg gtgcgggcag atatcagtcg
    32881 tatctcgatt gagctttttg acgccacctt acaaaaatgg tgtggcagcg aaaacccgca
    32941 accgcgccgt gactgccagg cgtgcgactg gcatcgcctg tgcccgcacg ctcgccagga
    33001 gacgccagac agcgtgcttt gtgcgggcta tcaggcgttt tattcttact ccgcaccgca
    33061 tatgcgggtg atgcgtgatc tgataaagca gcatcgttct cccatggagc tgatgacgat
    33121 gctgcggtga cgtgcgctta aacgggctgc ccctgatgcg acatccgcgt cgccagcggc
    33181 agccagcaca gcacgatcaa caatcccatt aaggtcatca acaaccccag gctgccctga
    33241 ccggtttgcg gcaacatcgc agagagcgac gccagcacgc cggaaccaat gttttgcaga
    33301 ccgccgacca gcgcgccagc cgtgcccgcc aggaagggga acggctccat cgcgccgctg
    33361 gtcgccagcg gaaacagcat cccggcaccg aaaaagaaca gcgcggcggg aacgagcagc
    33421 gtccagacat tcatcacgcc aaaccagtcg gggatccaca tcagcaagcc agccagcagg
    33481 cagcagataa ccgactgcca cattaacgtg gagaagcgtt tattgggacg tccggcaaac
    33541 catgcgccaa aaaacgctgc cggaatcggc agaataaaca aaatactgac cgtcatactg
    33601 ctcagcccta acaccgcgcc catcagcacg ccggagcagg cttcaaaggc ggcaatcccg
    33661 gccagaccgc caatcagcat cagcaaataa cagttaaaac cgctgttacc gaaaagcgtt
    33721 ttataactgg taagcaggcg cgtgcgcggt gcatcgaccg gacgcgtttc cggcatccag
    33781 cgggccatac tgaaggtcac accagcacaa agaaccaaca aaaagagata acaggcgcgc
    33841 cagttccaca tcgtatccag cagaccgccg attagcggtg cgagcaacgg actgacgaga
    33901 atccccatgt ttaacaggct gttagcatgg cgcaactgtg tccgttcata taaatctcgc
    33961 ggcaaagtac gcgccattac gccgccaacg ccggtgccca tcccctgcat cgcgctggcg
    34021 gcaatcaaca ccgtcaaact ggaggtcgtg accgcgacca gcgttgccag cataaaaatg
    34081 gacattccga cgaggatcac cggtcggcgg cccacgcggt cggaaatcgg gccataaaac
    34141 agctgtgaga caccgtaagt cagcagataa gcgcccatta cgctctgcac cgccccttca
    34201 cggacgttga gatcgcgcgc catatcggca atagctggaa tataaatggt ttgcgccatc
    34261 tgaccgacgg ccacgagtaa taccaacatc aataacaaat tgacgtttct ttgccttttc
    34321 attatcacgg atgcttttat aaaaataatt agaataagtc actacgcatt cccataaatg
    34381 cgggagagga atctatcaca ttgatgaatg ttagccagat atacgccagg aatggggaat
    34441 tgtttagcgg cagaatatgt aaacaaaagc ggcaataaat gttgccggga gggattacgt
    34501 gcgaactgta accctctccc caaaggggcg agggggaaac caccgttccc tttcagaaaa
    34561 tgagctaaac attgcacggg acggtcccct cgcccctttg gggagagggc tagggtgagg
    34621 ggcacaggca gcgcaggtgc aaacattctg ctggtagaag aagcccccaa ctccaacccg
    34681 cacgctaaat ataaaagggg agcggttacc cgctcccctt tggtgcgact tgaatctgaa
    34741 ttacttcagg tatttcagaa cagcatcaag cagttgcagt gctgcaacaa tgagtttgag
    34801 gataagaatg gcgatatcca ccaggctcat acgcgtctcc tgtggttcag gagcactgcc
    34861 agctgacgta cctttccgct gcctgttgcc agctgttgtg cacgcggtat cttaaggaga
    34921 aacgttgtgc ttgttgacat aacacagtgt gctcacgcgg ggttaaggcg ctgacggcac
    34981 cacccgtttc agccaggact tagtgcgccg ggtaacgatg cggcccccgc ataacacatt
    35041 gcgtacagtg atattataga agtttactgt ataaataaac agtaatattt ggacaaaacg
    35101 caaactgtga tcgatactca actatgccct aaatacgtca aaattcgtgc cgaaattgcg
    35161 cgttctgcgc ggaacacgta tactttcagt gttgacataa tacagtgtgc tttgcggtta
    35221 ccagccgcag gcggctgatg aaacctcgct ccggcggggt tttttgttac ctgcaattca
    35281 gtacaaaacg tgatcagccc ctcaattttc ccttgctgaa aaattttcca ttgtctcccc
    35341 tgtaaagctg tgcttgtata aatattgtta aacacaaaac caacaaggtc cccaatgact
    35401 acttccatgt tcaacgcaaa actactacca actgcgctat ccgccgcagt ggtcgtcgtg
    35461 cgtgtggtgg tggtcgtcgg caatgcgccg tagggactgg aacaacacac gattccaaaa
    35521 ccccgccggc gcaaaccggg cggggttttt cgtttaagca cctcccggaa agtcggccca
    35581 gaagaaaagg actggagcat ggcaagttcg ggcacaacat cgacgcgtaa gcgctttacc
    35641 ggcgcagaat ttatcgttca tttcctggaa cagcagggca ttaagattgt gacgggcatt
    35701 ccgggcggtt ctatcctgcc tgtttacgat gccttaagcc aaagcacgca aatccgccat
    35761 attctggccc gtcatgaaca gggcgcggga tttatcgctc aggggatggc gcgcaccgac
    35821 ggtaaaccgg cggtctgtat ggcctgtagc ggaccgggtg cgactaacct ggtgaccgcc
    35881 attgccgatg cgcggctgga ctccatcccg ctgatttgca tcaccggtca ggttcccgcc
    35941 tcgatgatcg gcaccgacgc cttccaggaa gtggacacct acggcatctc tatccccatc
    36001 accaaacaca actatctggt cagacatatc gaagaactcc cgcaggtcat gagcgatgcc
    36061 ttccgcattg cgcaatcagg ccgcccaggc ccggtgtgga tagacattcc taaggatgtg
    36121 caaacggcag tttttgagat tgaaacacag cccgctatgg cagaaaaagc cgccgccccc
    36181 gcctttagcg aagaaagcat tcgtgacgca gcggcgatga ttaacgctgc caaacgcccg
    36241 gtgctttatc tgggcggcgg tgtgatcaat gcgcccgcac gggtgcgtga actggcggag
    36301 aaagcgcaac tgcctaccac catgacttta atggcgctgg gcatgttgcc aaaagcgcat
    36361 ccgttgtcgc tgggtatgct ggggatgcac ggcgtgcgca gcaccaacta tattttgcag
    36421 gaggcggatt tgttgatagt gctcggtgcg cgttttgatg accgggcgat tggcaaaacc
    36481 gagcagttct gtccgaatgc caaaattatt catgtcgata tcgatcgcgc agagctgggt
    36541 aaaatcaagc agccgcatgt ggcgattcag gcggatgttg atgacgtgct ggcgcaattg
    36601 atcccgcagg tggaagcgca accgcgtgca gagtggcacc agttggtagc ggatttgcag
    36661 cgcgaattcc cgtgtccaat cccgaaagcg tgcgatccat taagccatta cggcctgatc
    36721 aacgccgttg ccgcctgtgt cgatgacaat gcgattatca ccaccgatgt gggtcagcat
    36781 cagatgtgga ccgcgcaagc ttatccgctc aatcgcccac gccagtggct gacctccggt
    36841 gggctgggca cgatgggttt tggcctgcct gcggcgattg gcgcggcgct ggcgaacccg
    36901 gatcgcaaag tgttgtgttt ctccggcgac ggcagcctga tgatgaatat tcaggagatg
    36961 gcgaccgcca gtgaaaatca gctggatgtc aaaatcattc tgatgaacaa cgaagcgctg
    37021 gggctggtgc atcagcaaca gagtctgttc tacgagcaag gcgtttttgc cgccacctat
    37081 ccgggaaaaa tcaactttat gcagattgcc gccggattcg gcctcgaaac ctgtgatttg
    37141 aataacgaag ccgatccgca ggctgcattg caggaaatca tcaatcgccc tggcccggcg
    37201 ctgatccatg tgcgcattga tgccgaagaa aaagtttacc cgatggtgcc gccaggtgcg
    37261 gcgaatactg aaatggtggg ggaataagcc atgcaaaaca caactcatga caacgtgatt
    37321 ctggagctca ccgttcgcaa ccatccgggc gtaatgaccc acgtttgtgg cctttttgcc
    37381 cgccgcgctt ttaacgttga aggcattctt tgtctgccga ttcaggacag cgacaaaagc
    37441 catatctggc tactggtcaa tgacgaccag cgtctggagc agatgataag ccaaatcgat
    37501 aagctggaag atgtggtgaa agtgcagcgt aatcagtccg atccgactat gtttaacaag
    37561 attgcggtgt tttttcagta accgctcaag gcttgaacaa catcgcgctt atcgttaagg
    37621 taagcgcgtt tttttttacc cgccaggaca agaccatgat caccgttgcc cttatagacg
    37681 atcacctcat cgtccgctcc ggctttgcgc agctgctggg gctggaacct gatttgcagg
    37741 tagttgccga gtttggttcg gggcgcgagg cgctggcggg gctgccgggg cgcggtgtgc
    37801 aggtgtgtat ttgcgatatc tccatgcccg atatctccgg tctggaactg ctaagccagc
    37861 tgccgaaagg tatggcgaca ataatgctct ctgttcatga cagtccggcg ctggttgagc
    37921 aggcgcttaa cgcgggggca cgcggctttc tctccaaacg ctgtagccct gacgaactga
    37981 ttgcggcggt gcatacggtt gccacgggcg gctgttatct gacgccggat attgccatta
    38041 aactggcatc cggtcgtcag gacccgctga ccaaacgtga acgccaggtg gcggaaaaac
    38101 tggcgcaagg aatggcggtg aaagagattg ccgccgaact gggcttgtca ccgaaaacgg
    38161 tacacgtcca tcgcgccaat ctgatggaaa aactgggcgt cagtaacgac gtagaactgg
    38221 cgcgccgcat gtttgatggc tggtgatgaa gacgttgttc tcccgcttaa ttaccgttat
    38281 tgcctgcttt tttatcttct ctgccgcatg gttttgcctg tggagtatca gcctgcacct
    38341 ggttgagcgc cctgatatgg cggtgctgtt atttccgttt ggtctgcggc tagggctaat
    38401 gctgcaatgc ccgcgcggat actggcccgt attgctgggt gcggaatggc tgctgattta
    38461 ctggctaacg caggcggtcg gtttaaccca ttttccgtta ttgatgatcg gtagtttact
    38521 gacgttactg cccgtagcgc tgatctcgcg ctatcgccat cagcgtgact ggcgcacctt
    38581 gctgttacag ggggcggcgt taacggcggc ggcgttgttg cagtcgctgc cctggctttg
    38641 gcacggcaaa gagtcgtgga atgcgctgtt gctgacttta actggcggcc tgacgctggc
    38701 cccgatatgt ctggtgttct ggcactatct cgccaataac acctggctgc cgctcggccc
    38761 gtcactggtt tctcaaccga tcaactggcg cgggcggcat ctggtctggt acttgctgtt
    38821 gtttgttatt agtctctggc tccagttggg attgccggac gaactgtcgc gctttacgcc
    38881 attctgcctg gcgctgccga ttatcgcgct ggcctggcac tacggttggc aaggggcgct
    38941 gatcgcgacg ttgatgaacg ccatcgcgct gatcgccagt caaacctggc gcgatcatcc
    39001 ggtggattta ttgctctcgt tgctggtgca aagtctgaca gggttgttac tgggcgctgg
    39061 catccagcgg ttgcgtgaac ttaaccagtc gctgcaaaag gaactggcgc gcaatcagca
    39121 tctggctgaa cggttgctgg aaaccgaaga gagcgtgcgc cgtgatgtgg cgcgtgagct
    39181 gcatgatgat atcggtcaga ccatcacagc cattcgtact caggcgggca ttgttcagcg
    39241 gctggcggca gataacgcca gcgtgaagca gagcgggcag ctcatcgaac aactgtcgct
    39301 gggcgtgtac gacgcggtgc gccgtttatt aggacggtta cgtccgcgcc agttggatga
    39361 tctctccctg gagcaggcca tccgctcact gatgcgggaa atggagctgg aagggcgcgg
    39421 cattgtcagc catctcgaat ggcgaatcga tgaatcagca ttaagtgaaa accagcgcgt
    39481 gacgctgttt cgtgtctgcc aggaagggct gaacaacatt gtgaaacatg ctgatgccag
    39541 cgcggtcacc ctgcaaggct ggcagcagga tgaacggttg atgctggtta ttgaagacga
    39601 tggcagcggt ttgccgccgg gttccgggca acaaggtttt ggcctcaccg gaatgcgcga
    39661 gcgcgtaacg gcgctgggtg gcacattaca catttcctgt ctgcacggca cgcgtgtcag
    39721 cgtttctcta cctcaacgct atgtctaagg tttgatgatg ttgccgtttc tgaaagcgcc
    39781 tgccgatgcg ccattaatga ctgataaata tgaaattgat gcccgctatc gctactggcg
    39841 tcggcatatt ctgctgacca tctggctggg ttacgcgctg ttttacttca cgcggaaaag
    39901 ttttaacgcc gccgtaccag aaatccttgc taacggcgtg ctcagccgta gcgatatcgg
    39961 cctgttagcg accctgtttt acattactta cggcgtgtcg aagtttgtct ccggcattgt
    40021 cagcgatcgc tcaaatgccc gttattttat ggggataggg cttatcgcca cgggcattat
    40081 caacattctg tttggcttct cgacgtcgct atgggcgttt gccgtgctct gggtgctgaa
    40141 cgcctttttc cagggctggg gttcaccggt gtgtgcgcgt ctgttaacgg cctggtattc
    40201 acgtaccgag cgcggcggtt ggtgggcatt atggaacacg gcgcataacg tcggcggcgc
    40261 actcattccc attgtgatgg cagcggctgc gctgcattac ggctggcgtg ccgggatgat
    40321 gattgctggt tgtatggcga tagtcgtggg gatttttctc tgctggcggc tacgcgatcg
    40381 cccgcaggcg ttaggtttac cggcggtcgg tgaatggcga cacgacgcgc tggaaattgc
    40441 tcaacaacaa gaaggggcag ggttgacgcg taaagagatc ctcaccaaat atgtgttgct
    40501 gaatccgtat atctggctgc tttcgttttg ctatgtgctg gtctatgtgg tccgggcggc
    40561 gatcaacgac tggggcaatt tgtatatgtc cgagacgctg ggcgtcgatc tggtcacggc
    40621 gaatacggca gtgacgatgt ttgaactggg tggatttatc ggtgcgctgg tagcgggttg
    40681 gggctcggac aaattgttta acggcaaccg agggccgatg aatttgattt tcgccgccgg
    40741 aattttgctt tcagtcggct ccctgtggct gatgccattt gccagctacg tgatgcaggc
    40801 aacctgcttc ttcaccattg gtttttttgt ctttggccca cagatgttaa tcggtatggc
    40861 ggcggcagag tgttcccaca aagaggcggc aggggcggcg acggggtttg tcggcttgtt
    40921 tgcttatctg ggggcgtcgc ttgctggttg gccgctggcg aaagtactcg atacctggca
    40981 ctggagcgga ttttttgtgg ttatctctat cgccgccggg atttccgcac tgctgttact
    41041 gccctttttg aacgcccaga caccgcgcga agcgtgatgc atctcacctt ttcacttcat
    41101 atccggcaaa actaagaaat tttccaggtt ttgcctggac gctatctcag gcctgatttg
    41161 ctgctgattt ttacaatgca tgcctcacgc aggtattcat ttcaggagta acccatgctg
    41221 gctttcttaa accaggttcg caagccgacc ctggaccttc cgctcgaagt gcggcgcaaa
    41281 atgtggttca aaccgttcat gcaatcctac ctggtggtct ttatcggcta cctgacgatg
    41341 tacctgattc gcaagaactt taacatcgcg cagaacgaca tgatctcgac ctacgggttg
    41401 agcatgacgc agctggggat gatcggcctg ggtttctcca tcacttatgg cgtgggtaaa
    41461 acgctggttt cctactacgc cgacggcaaa aacaccaaac aattcctgcc gttcatgctg
    41521 atcctgtctg ccatttgtat gctgggcttc agtgccagta tgggcagcgg ctcggttagc
    41581 ctgttcctga tgattgcctt ctacgcctta agcggctttt tccagagtac cggcggttcg
    41641 tgcagttact ccaccatcac caaatggacg ccgcgtcgta aacgcgggac attcctcggt
    41701 ttctggaata tttctcacaa ccttggcggt gcaggcgcag caggtgtggc gctgttcggg
    41761 gcaaattacc tgttcgatgg ccatgtcatc ggcatgttta tcttcccgtc gattatcgcg
    41821 ctgattgtcg gttttatcgg cctgcgttac ggcagcgact ccccggaatc ttatggcctc
    41881 ggcaaagctg aagaactgtt cggcgaggag atcagcgaag aggacaaaga gacagaatct
    41941 accgatatga ccaagtggca gatctttgtt gagtatgtgc tgaaaaacaa agtgatctgg
    42001 ctgctgtgct tcgccaacat tttcctctat gtggtacgta ttggtatcga ccagtggtca
    42061 accgtatacg cgttccagga actgaaactc tctaaagcgg tggcgattca gggctttacg
    42121 ctgtttgaag ctggtgcgct ggtcggtacg ctgctgtggg gctggctctc tgacctggcg
    42181 aacggtcgcc gtggcctggt ggcctgcatc gcgctggcgc tgattatcgc cacgctcggt
    42241 gtgtatcaac atgccagcaa cgaatatatc tatctggctt ctctctttgc gttgggtttc
    42301 ctggtctttg gcccgcaatt gttgattggt gtggctgctg ttggctttgt acctaaaaaa
    42361 gcgattggcg ctgccgatgg tattaaaggc acctttgctt acctgattgg tgacagcttt
    42421 gccaagttag gtctgggaat gattgccgat gggacgccgg tattcggcct taccggctgg
    42481 gcaggcacct tcgccgcact tgatatcgcc gcgattggtt gtatctgcct gatggcgata
    42541 gtggcggtga tggaagaacg caaaatccgc cgcgagaaaa aaattcagca gttgacagtg
    42601 gcataaacgt aactggtgac ttttgcccgg catgacgccg ggctttttta ttattccgtg
    42661 acttccagcg tagtgaaggc aaacttctcg ccatcaaata gcccctgact ggttagtttt
    42721 agcgcgggga tcactggcag agaaagaaac gccatctgaa taaacggctc atcgggtaac
    42781 ggaccgcatt cacgggcggc ggctttcaag gcgtcaattt gttccgccag caactgcgcc
    42841 gtgtcggtgc tcatcaggcc ggcaatgggc aacggcagat gactttggac ctggccgtta
    42901 cgtaccacgc acagcccgcc gccatcctga atcacctgat tgaccgccag cgccatctct
    42961 tcggcactgc gaccgatcac cacaatatta tggctgtcat ggctgaccgt cgcagccagc
    43021 gcaccttcat tcagtccaaa gccgccaagc aaaccacaag ccggagccag ccgttgcccg
    43081 taacgctcaa gtacggcaat aaagctcaca tcatcgcgat caaaaccatt ttcgctgtag
    43141 acgctggagt gggagtgcgt aatcaattcg ttatggatga cgtcaatgac ccgatagcgt
    43201 tttccgggcg taaattgcag ggcaaagtcg ctggcggaaa ctggctggcg ggcaatggtg
    43261 ttgccatatg gcggagcgga ttgtgccagt ctcgccgact cttccgcctg taaggtttgc
    43321 gcatcaatcg gctcgccttt caccagtacc tgctgcaccg tgaccttacg cgcatcgctc
    43381 aacaggacga tatcggcctg cttgccgggt gccagtaagc cgaggtgatt cagaccaaag
    43441 tggcgcgccg tcgaccagct ggcgacgcga tatgccacat gcagcggcac attgtgttgt
    43501 tcgatcaggc ggcgaattaa ggcatcgatg tgtccttcat gggcgatctc ccacgggtta
    43561 cggtcatcgg tacagagcat gcattgcggg ctgttaaatt cgttgatcaa cggtgccagc
    43621 gcgttgagat tgcgggcagc ggacccttcg cggatcatca acgacatgcc gagttgtaat
    43681 ttccggcgtc cttcttccag ctgataactt tcgtggcagt tttcaatacc cgcagtaata
    43741 taggcgttaa gttctttacc acccaaaccc gggcagtgac cgtccagcgt caggtggcga
    43801 aatgcatcca gtttatcgag cagcgcattc tgcccgctaa ttacgccagg gtagtccatc
    43861 atttctgcaa ggccggtaac ctgcggatgg tcccgccagg cgagcatctg ttcaagggta
    43921 aaactggcac cgttaacatc gcagccttcc agggcgggta cgcaagagct gacctgtaag
    43981 tactggtttt gccttgcctg ttcggcacag cgggcaaacc aggcgaatcc ggcttctccc
    44041 atcacgttga cgatttcatg agggtcgcaa ataacggtcg tcaggccgcg cggcagggta
    44101 gcggtttcaa aagtgaccgg cgtcatcatg ctggattcaa tatgcaggtg agcatcaata
    44161 aaccctggca ccgccgttgc gccgcgagca tcaatccgct gcaaagccgg agcatcagcg
    44221 tattctgcgc caacaccggc aatgtaacgt cctttaatca caattgggcc ggaaatttct
    44281 ccgccattga tcaggtcgag aatagagaca ttatcaataa tataatcggc aacagcgtcg
    44341 ccacgggaaa cggctaacaa ttcctggtat tcagcccggc taatgtgatg aaatttatgg
    44401 ttaatagaat tattcatatt tttctcattt tgattttatc aatttgatta ttatttatca
    44461 taaagaaacg tcgcgagatt attaaagaat atccattaat gtgcaattga aatgtgataa
    44521 ttatcactga accgttgttg tcatttgttt tacaaaagca agggattaaa tctcctccaa
    44581 tggacaaaaa aatgaataat gacaataccg attacgtgag taatgaatca gggacgcttt
    44641 cgcgattatt taaactacct cagcatggga ccaccgtccg cacagaattg attgcgggga
    44701 tgaccacttt tttaaccatg gtgtacatcg tttttgtgaa cccgcaaatc ctcggcgcgg
    44761 cacaaatgga cccgaaagtg gtgtttgtta ccacctgttt gattgccggt atcggcagta
    44821 ttgcgatggg gatatttgct aacttacccg tggcgctggc tccggcaatg gggctgaacg
    44881 ccttctttgc cttcgtggtc gtgggggcga tgggcatctc ctggcagacc gggatgggcg
    44941 caatattctg gggcgcagtt ggactatttt tgctcacgct gtttcgtatc cggtactgga
    45001 tgatctccaa cattccctta agtttacgta ttggtatcac cagcggaatt ggattattta
    45061 tcgccttaat gggattaaaa aatactggcg ttattgtcgc caataaagac acgctggtga
    45121 tgattggcga tttaagttct cacggcgtgt tgttaggtat tttagggttt tttattataa
    45181 ccgtgttgtc atcacgtcat tttcatgccg cggtgctggt ttctattgtg gtgacgtctt
    45241 gctgtggatt atttttcggt gatgttcatt ttagcggcgt ctattccatt ccgcctgata
    45301 ttagcggcgt cattggtgaa gtagatttga gcggcgcgtt aacacttgaa ctcgccggta
    45361 tcattttctc ctttatgctg atcaacctat ttgattcatc aggaacatta attggtgtaa
    45421 ctgataaagc gggcttaata gatagtaacg gtaaattccc caatatgaat aaggcgctgt
    45481 atgttgatag cgtcagttcg gtggcggggg cgtttatcgg cacctcgtct gttactgcct
    45541 atattgaaag tacttctggt gtggcagtcg gtggtcgcac ggggctgact gcggttgtgg
    45601 ttggcgttat gttcctgtta gttatgttct tctcaccgct ggtggcgatg gttcctcctt
    45661 acgcaaccgc cggagcgtta atctttgttg gcgtgctgat gacttcgagc ctggcgcgcg
    45721 ttaactggga tgattttacc gaatcggtgc ctgcgtttat taccacggtg atgatgccct
    45781 ttactttctc gatcaccgaa gggattgcac tcggctttat gtcgtactgc atcatgaaag
    45841 tatgcaccgg gcgctggcgc gatctgaacc tgtgtgtggt ggtggtcgca gctctgtttg
    45901 cactgaagat tattctggtg gattagtaac tggcgagggt ttccagatat catgagttct
    45961 gattacgcag gagaactcat gatctggata atgctcgcca cgctggcggt agtgtttgtg
    46021 gttggttttc gggtgctgac atccggggcc agaaaagcga ttcgccgtct cagtgatcgg
    46081 ctgaatatcg atgtcgtacc cgtggagtcg atggtcgatc aaatgggaaa gtcagccggt
    46141 gacgaatttt tacgttattt gcatcgcccg gatgagtcgc acctgcaaaa cgccgcgcag
    46201 gtgttgctca tctggcaaat tgtcattgtc gatggtagcg aacagaacct gctgcaatgg
    46261 catcggattt tacaaaaagc tcgcctcgcc gcgccgatta ccgacgctca ggtcaggctg
    46321 gcgctgggtt ttctgcgcga aaccgaacct gaaatgcagg atattaatgc ttttcagatg
    46381 cgctataacg cgttctttca gcctgccgag ggcgttcact ggttgcattg aaaccgtttc
    46441 gtcttatcag cctacagacc gcgcctgacc tgtcgggtgg ataagacgtt cacgccgcat
    46501 ccggtaatct ttgccctgtt ccgtttcgat cttaactcca gctcaagcaa caccgtaaaa
    46561 gttgcaaaaa cgttaaatgc gtcacacatt tcaatgttaa agtggtcggc ttttcccctg
    46621 aaacatgcca cgggtaacac catgagtgaa tttattgccg aaaaccgcgg cgcggatgcc
    46681 atcacccgac cgaactggtc agccgttttc tcggtggcgt tttgtgtcgc ctgtctgatt
    46741 atcgttgagt ttttgcccgt cagtttgttg acgccaatgg cccaggattt aggcatttcg
    46801 gaaggggttg ccgggcaatc ggtgaccgtg accgcctttg tggcaatgtt tgccagtttg
    46861 tttattaccc agacaattca ggctactgac cgccgctacg ttgttatttt gtttgccgtt
    46921 ttgctgacgc tctcctgctt gctggtttcc tttgctaact cattcagttt gcttttaatc
    46981 ggtcgtgcct gtctggggct ggcgctgggc gggttctggg cgatgtcggc gtcgctgacc
    47041 atgcgtctgg tgccgccgcg tacggtgccg aaggcgctgt cggtgatctt cggcgcggtt
    47101 tctattgcgc tggtgattgc cgcgccgttg ggcagttttt taggcgagct tatcggttgg
    47161 cgcaatgtct ttaatgcggc ggcggtgatg ggcgtgctgt gtattttctg gattatcaaa
    47221 tcattgcctt cactgccagg cgaaccctcg catcagaaac aaaatacttt ccgcttatta
    47281 caacgtccgg gtgtgatggc agggatgatc gccatcttca tgtctttcgc cgggcagttt
    47341 gctttcttca catatattcg cccggtgtat atgaacctgg cgggattcgg cgtggatggc
    47401 ttaacgctgg tgctgttgag ttttggcatt gccagcttta tcggtacgtc gctttcgtca
    47461 ttcattctta aacgttcggt aaaactggcc ttagcaggcg cgccgttaat actggctgtg
    47521 agtgcgttgg tgctgacgtt gtggggaagc gataaaatcg ttgctaccgg cgtggcgatt
    47581 atctgggggc taacttttgc attggttccc gtcggctggt caacgtggat cacccgctcg
    47641 ctggccgatc aggcagaaaa agccgggtct attcaggtgg cggttattca gcttgctaat
    47701 acctgtggcg cggcaatcgg cggttatgcg ctggataata ttggtctgac ttcgccgttg
    47761 atgttgtccg gcacattgat gttgctgact gcattgttgg ttactgcaaa ggtgaaaatg
    47821 aagaaatcct gatatcaggt aaagaagcgt tggtcagtag ttgttgctgt tcttacatat
    47881 ccgggcattc taccttaccc aacgcttccc aataattgct ctgattatta cgttccatcg
    47941 ccacgatggc ctcgcgtata tgttgtttat tgttttcatc tgacaacatt aacttttccc
    48001 acgcttcatc tgataatgac gcctgtggcg tgcaaatttt acgtaaatct tgtaataccg
    48061 tggttttctc ttttgcggac tgcagtgagc taaaaggcga ctcggcgtat acgatgcctg
    48121 gcatagcgaa aaaagtgaaa ataagcagta gtgtcgttgg tttcatggat tcaacctttt
    48181 acaaatgtgt atcgaatgaa gcataagtgt agtttgcttt catcgctctc atcatgctgt
    48241 acgaaacgcg cttgcgtggt cagtaagaag tgccagactt tatattccac ttttattcct
    48301 ttttattctt atcgatagcg tttcgttttt taaaccgcag cgaccttacc gctatagtca
    48361 ggtaatcatt aataaaagga taaaaaaatg aaactgacaa cacatcatct acggacaggg
    48421 gccgcattat tgctggccgg aattctgctg gcaggttgcg accagagtag cagcgatgca
    48481 aaacacatta aagttggcgt tataaatggc gcagaacaag atgtcgcgga agtcgctaaa
    48541 aaggtggcaa aagagaaata tggcctcgat gttgaactgg taggatttag cggttcgttg
    48601 ctaccgaacg atgcgacaaa tcatggcgaa cttgatgcga atgtcttcca gcatcgaccc
    48661 tttcttgaac aagataatca ggcgcatggt tataaactgg tagcggtggg aaatacgttt
    48721 gtcttcccta tggcgggtta ttccaaaaaa atcaaaaccg tggcgcaaat aaaagaaggc
    48781 gcgacagtgg cgatccccaa cgacccgacc aaccttggtc gcgcactttt actgttgcaa
    48841 aaagagaagc taattactct gaaagaggga aaagggttat tacctaccgc gctggatata
    48901 accgataatc ctcgtcattt gcagattatg gaactcgagg gggcgcagct cccgcgcgta
    48961 ctggatgatc ccaaagttga tgtagcgatt atcagcacca cttacattca gcagacaggg
    49021 ctttctccgg tgcacgacag cgtatttatt gaagataaga attcgccgta tgtgaatatt
    49081 ttggtggcac gggaagataa taagaatgca gaaaacgtga aggaatttct gcaatcttat
    49141 caatcacccg aagtcgctaa agcggcagaa accattttta acggtggcgc ggtgcctggc
    49201 tggtaattct cactcatgcc gcgccggacg atttattttt ctggcgcggc gacgggaatc
    49261 cattgaaatc aaaatcaccg atgacagaat cagcagcgtt cccagccagt ccggtaatgt
    49321 gaacgtgatc cccagcaaca gcaaagagag tagcgcgcta cttaatggtt ctgcacagct
    49381 caaaatgctg gcttttggac cgccaattaa ttgtgctcct ttcaggtaca gactaaatgt
    49441 cagggacgta ccaatgacca ccaaataaaa aaacgacaga atcaaactgc cgttaacgac
    49501 aaagtttgtt ccttgtctgg cataaaaagg caacagaatc agaccgccaa tcagcatact
    49561 ccagccgacg actggtaacg tgccatagcg ggcaattagc gttgagggat aggtggtata
    49621 gaatgcagca gcaaatgccg aggcaatgcc ccagaacaac gcggcaggag agatcgataa
    49681 tgacgtcgga ttaccgtgtg tcaccaataa aaaagtcccg accagcgatg tcaaaatagc
    49741 gcagaaaacc agaatgcccg ggcgcgattt acgcaccagt gagaaccagg cgacgataat
    49801 cgtcggtgag aggaattgca gcaccgttgc cgtggctgcg ttcgattttt cgatggttag
    49861 caaaaaagtg agctgtacag ttagcgcgcc aaccacggaa aaaatcagca ggctaatggc
    49921 atctttatga ttgttaataa tagaaaagat tttatcgcca tgaacaaatg acagcgtcag
    49981 tagaatcaaa ccggcgaata tcaaacgcgt catagtcaaa aactgcgacg acatctggct
    50041 ttgctccatg atgtattgcg cgcagacccc tgaacttccc cacaacacgg cggcaatcag
    50101 aacgttcagc atcccctttc tggtggaacc catttttccc tctgcatact gttttgtcgt
    50161 ttatgtatgc aggcatgata gcaaaaatgg cgaggatggc tctgtttgcg ccacgccgat
    50221 ttttcttagc ccagactggt gagctaaata tctttaataa acagcaggca aatcatcgca
    50281 atacccaaca tccctatcgc cagacagaac aacgcatggt agctccagat ttcaaccata
    50341 attccgtcaa cagagccggc gataatccag ccgacgcgtg aagtatttgc atataacgtt
    50401 gtcgcagagc ctattttttc aggcatcaag tcctgaaaat aaagcatgcc gataccacag
    50461 agaataccaa ggaagatggc attaagaatt tgcaattcca gctcaaccgc cggagtcgtc
    50521 gccatgagta cgctggcgta aaaacacatt ccactcacga tagcaatgag cattaatagt
    50581 cgcttgccaa tacgtttcat gtaatagcct aacatcatcg gaatttccag accggcagcg
    50641 ataccaatca tttctccagc cagtttatcg gttagatgca gttcatcaat aataaatagc
    50701 ggcatattta tcatgtagag attattcgcc gcccacatca ttgaacagac cacaaaaagt
    50761 agccgcgtat cccgcttcct gtgggtggag ggtaaaattt ctacgggttg ggtaacgaca
    50821 ggaatatttc tttgtattga tggcaaaaac aaccagacta tcagtccgca aacaacaaat
    50881 gcgatggctg cggtgagata catcacttta aaactaaatc ccattgccag ttcataagcg
    50941 agcggtggcc cgataaccca ggcaagcgag atctgagcac gtaaaaatgt actgaacatg
    51001 accgtctcac ggcctgttct gtcggcgtgt tcacgggcga gggcgaacat ttgcgggttt
    51061 gcggtggaag caaaactact cagaagtacg cccgttgaga ggagaatgaa gtagttgcga
    51121 ttccacgcaa aaagcgtgca ggccagcact ccaaataagc aacatagcag aatcagtaat
    51181 ttacggtcgc cttgtttatc ggagtgcctt gccagaaatt gactgaccag aattcccata
    51241 atagcgctac cggtgaagaa aaaacctacc attataggac gggctttcag ttcatctgcg
    51301 aggaatatac ttagggtagg agtctgaaga gcgcccgcaa tacccgtcag aaaggcgaca
    51361 agtaaaaatg ccgcggcagt gagatcaagt atttttgatg gagtggtagc cgttttttgc
    51421 attcttctga atcaccgggc gaaaaaatat aactgagtta caaataagta ccagtcgaat
    51481 gagtattgta ttaagaattt ttgagtatcg ctgatacaac cagaaagcaa tgctagcgca
    51541 atattatagg gccagcaatt gagattatta gtaaaagcat gttaagaatt aattaccgaa
    51601 tttatatatt aattatagta aggcaatgag ctatttttga tagtaaaaat aaaacaagtt
    51661 gatttgatat attaaatgaa caaattaatc ttgatggcag tctgattatt ggtgaagttt
    51721 atagtcagtt tttttcgcca gcggttaaag aaatagaata gtcgtataaa aaagggtgtt
    51781 ctggggcgag taaaacaaca tcttcacctt aatggcgata cagttggctg agagtcagca
    51841 ggggggcaaa tcacctgcaa ataaacacgg cgcatactgg cgtgaatatc gcggatgcgt
    51901 tcgaggacag aagaaccgac aggcggattc ttcagccgct catcgtccat cacaaaacct
    51961 tagaccaggt attcctgaag ccgattgaat attgagcagt agaaaatatc tggattgaca
    52021 gtattacaag gggttggaga acgtcaggag aggcattttg gcggagatca caggagtcga
    52081 atatgagtgt taatgttttg attttaaatg attaatctgt gttggataaa taagtaaccc
    52141 ccataagaac ccccaaatga ctttgtgtaa gggaatgaag ctaaaataca caatcgaatc
    52201 aattcttaga gctttattat gaaggtaaca ctcccggata gtgtttcgca ataatatcat
    52261 tcagcctgtc aaccagttct ggtttcctga acctgatgtg cgcggagcct ttcttaaagt
    52321 atctgatgat gaacatctca tcttcatagc actctttacc ctgaacagac cggatgtgat
    52381 catccagatg aatggtgatg ttttcccggt tgtcggggat cggtttaccg ctgaacaggt
    52441 gcaacattct ttccagatcg gcgagtcggt cagcctgttg cccggtgata agatgaaatc
    52501 cccaccggtc ccaccttacc aggttgttaa tgataatttt actgccaaat ttacaggggc
    52561 aattagtttt gtaattccag ctcagtcccc tgaagacgtt gatcacccct cgctcaaaca
    52621 cttcatcttt gttctggtgc agttgtttaa atgtgctcag aatgttcgct tcgctgattt
    52681 ccggaaaatt atcgtattcc agtgaccggt accatgtctc acgggcctgc gcatccatta
    52741 atgacagcat cccggaacgt tgcatcaggt cacgccagat ttcgcgatcg agattgcggg
    52801 tgatggcttt cattgccgtt tcaggttttt ccatcagcca gcaaccacag cgaaaatcct
    52861 gtttcatcgc ccagtccagt gcggttttac cgccaatact ttctgtcatt gctgagattt
    52921 ctgccagttg ccgggtaagt gtgtcgattt gcagcagcgt actcttttgt acagccagaa
    52981 tgtggcggat actggttgag cagataacgt ccgtatgccc gttgaccact tccggacggg
    53041 gggctgtgat ttcagacata atcgatattc ctttttgcag ataaatgaaa acgccagccc
    53101 tgttcaggct ggcgtcagtg gaatgaagtc tggtgagtaa gactcaccgg ttctggtaca
    53161 gaagctgcag ttggcgcagg gtacggatat cctccctggc ctgttcaata cagggcaaca
    53221 gtgctgccga atccgttttg tcctcatcgt tgtcaaagat aatttccgat tcgcagtcaa
    53281 tattgtcctg cagccacgca atgaggatat ccagcgctgt ttcagtggtt aatggcatca
    53341 cgctctgtat cactgtattg tcttccggca actgatgagg gctggcagaa gttgccggtg
    53401 tttcggtagc gtcatgttca tccaccacgc ggagtgaata accgttttca gcgactttgt
    53461 taatcagttc tgcgagctta ataccatcga cgtcaacgac aatgcgcccc atgttcagcg
    53521 cctgcacgtt aacgctgtct gcctccagcg tcagggccag tttcattgtt tcgcctccgg
    53581 atgcttcccc tggctaatgt catttaccat tctgtaattg tcgcgggtca tcaggccggt
    53641 cgccc
//
LOCUS       DAZFRN010000013        53225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFRN010000013 DAZFRN010000000
VERSION     DAZFRN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.013"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            2..169
                     /gene="yhfL"
                     /locus_tag="ACHKUB_000751"
     CDS             2..169
                     /gene="yhfL"
                     /locus_tag="ACHKUB_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO6653809.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            464..1801
                     /gene="frlA"
                     /locus_tag="ACHKUB_000752"
     CDS             464..1801
                     /gene="frlA"
                     /locus_tag="ACHKUB_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO6653810.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1822..2844
                     /gene="frlB"
                     /locus_tag="ACHKUB_000753"
     CDS             1822..2844
                     /gene="frlB"
                     /locus_tag="ACHKUB_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO6653811.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2894..3724
                     /gene="frlC"
                     /locus_tag="ACHKUB_000754"
     CDS             2894..3724
                     /gene="frlC"
                     /locus_tag="ACHKUB_000754"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO6653812.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3721..4506
                     /gene="frlD"
                     /locus_tag="ACHKUB_000755"
     CDS             3721..4506
                     /gene="frlD"
                     /locus_tag="ACHKUB_000755"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO6653813.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIITLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4606..5337
                     /gene="frlR"
                     /locus_tag="ACHKUB_000756"
                     /gene_synonym="yhfR"
     CDS             4606..5337
                     /gene="frlR"
                     /locus_tag="ACHKUB_000756"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO6653814.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5489..6574)
                     /gene="yhfS"
                     /locus_tag="ACHKUB_000757"
     CDS             complement(5489..6574)
                     /gene="yhfS"
                     /locus_tag="ACHKUB_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6653815.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6586..7890)
                     /gene="yhfT"
                     /locus_tag="ACHKUB_000758"
     CDS             complement(6586..7890)
                     /gene="yhfT"
                     /locus_tag="ACHKUB_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO6653816.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7902..8255)
                     /gene="yhfU"
                     /locus_tag="ACHKUB_000759"
     CDS             complement(7902..8255)
                     /gene="yhfU"
                     /locus_tag="ACHKUB_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO6653817.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8266..9144)
                     /gene="php"
                     /locus_tag="ACHKUB_000760"
     CDS             complement(8266..9144)
                     /gene="php"
                     /locus_tag="ACHKUB_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO6653818.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(9141..10367)
                     /gene="yhfW"
                     /locus_tag="ACHKUB_000761"
     CDS             complement(9141..10367)
                     /gene="yhfW"
                     /locus_tag="ACHKUB_000761"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6653819.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLI
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10367..11530)
                     /gene="yhfX"
                     /locus_tag="ACHKUB_000762"
     CDS             complement(10367..11530)
                     /gene="yhfX"
                     /locus_tag="ACHKUB_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO6653820.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHFFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(11614..11976)
                     /gene="yhfY"
                     /locus_tag="ACHKUB_000763"
     CDS             complement(11614..11976)
                     /gene="yhfY"
                     /locus_tag="ACHKUB_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO6653821.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11993..12898)
                     /gene="yhfZ"
                     /locus_tag="ACHKUB_000764"
     CDS             complement(11993..12898)
                     /gene="yhfZ"
                     /locus_tag="ACHKUB_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO6653822.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13167..14171)
                     /gene="trpS"
                     /locus_tag="ACHKUB_000765"
     CDS             complement(13167..14171)
                     /gene="trpS"
                     /locus_tag="ACHKUB_000765"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO6653823.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14164..14922)
                     /gene="gph"
                     /locus_tag="ACHKUB_000766"
     CDS             complement(14164..14922)
                     /gene="gph"
                     /locus_tag="ACHKUB_000766"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO6653824.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14915..15592)
                     /gene="rpe"
                     /locus_tag="ACHKUB_000767"
     CDS             complement(14915..15592)
                     /gene="rpe"
                     /locus_tag="ACHKUB_000767"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO6653825.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15610..16446)
                     /gene="dam"
                     /locus_tag="ACHKUB_000768"
     CDS             complement(15610..16446)
                     /gene="dam"
                     /locus_tag="ACHKUB_000768"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6653826.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16553..17839)
                     /gene="damX"
                     /locus_tag="ACHKUB_000769"
     CDS             complement(16553..17839)
                     /gene="damX"
                     /locus_tag="ACHKUB_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO6653827.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEAEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17931..19019)
                     /gene="aroB"
                     /locus_tag="ACHKUB_000770"
     CDS             complement(17931..19019)
                     /gene="aroB"
                     /locus_tag="ACHKUB_000770"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO6653828.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19076..19597)
                     /gene="aroK"
                     /locus_tag="ACHKUB_000771"
     CDS             complement(19076..19597)
                     /gene="aroK"
                     /locus_tag="ACHKUB_000771"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO6653829.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(19998..21235)
                     /gene="hofQ"
                     /locus_tag="ACHKUB_000772"
                     /pseudo
     CDS             complement(19998..21235)
                     /gene="hofQ"
                     /locus_tag="ACHKUB_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
     gene            complement(21147..21551)
                     /gene="hofP"
                     /locus_tag="ACHKUB_000773"
     CDS             complement(21147..21551)
                     /gene="hofP"
                     /locus_tag="ACHKUB_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO6653830.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21541..21981)
                     /gene="hofO"
                     /locus_tag="ACHKUB_000774"
     CDS             complement(21541..21981)
                     /gene="hofO"
                     /locus_tag="ACHKUB_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO6653831.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21965..22504)
                     /gene="hofN"
                     /locus_tag="ACHKUB_000775"
     CDS             complement(21965..22504)
                     /gene="hofN"
                     /locus_tag="ACHKUB_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO6653832.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22504..23283)
                     /gene="hofM"
                     /locus_tag="ACHKUB_000776"
     CDS             complement(22504..23283)
                     /gene="hofM"
                     /locus_tag="ACHKUB_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO6653833.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            23403..25955
                     /gene="mrcA"
                     /locus_tag="ACHKUB_000777"
     CDS             23403..25955
                     /gene="mrcA"
                     /locus_tag="ACHKUB_000777"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO6653834.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26120..26680)
                     /gene="nudE"
                     /locus_tag="ACHKUB_000778"
     CDS             complement(26120..26680)
                     /gene="nudE"
                     /locus_tag="ACHKUB_000778"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO6653835.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27000..29135
                     /gene="igaA"
                     /locus_tag="ACHKUB_000779"
     CDS             27000..29135
                     /gene="igaA"
                     /locus_tag="ACHKUB_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO6653836.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29200..29868
                     /gene="yrfG"
                     /locus_tag="ACHKUB_000780"
     CDS             29200..29868
                     /gene="yrfG"
                     /locus_tag="ACHKUB_000780"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO6653837.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29879..30280
                     /gene="hslR"
                     /locus_tag="ACHKUB_000781"
     CDS             29879..30280
                     /gene="hslR"
                     /locus_tag="ACHKUB_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO6653838.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30305..31183
                     /gene="hslO"
                     /locus_tag="ACHKUB_000782"
     CDS             30305..31183
                     /gene="hslO"
                     /locus_tag="ACHKUB_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO6653839.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31246..32970)
                     /gene="yhgE"
                     /locus_tag="ACHKUB_000783"
     CDS             complement(31246..32970)
                     /gene="yhgE"
                     /locus_tag="ACHKUB_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO6653840.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQVSEKV
                     LAENVLIAPGSGKPDAAFWSALIKERYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            33349..34971
                     /gene="pckA"
                     /locus_tag="ACHKUB_000784"
     CDS             33349..34971
                     /gene="pckA"
                     /locus_tag="ACHKUB_000784"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO6653841.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35047..36399)
                     /gene="envZ"
                     /locus_tag="ACHKUB_000785"
     CDS             complement(35047..36399)
                     /gene="envZ"
                     /locus_tag="ACHKUB_000785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO6653842.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36396..37115)
                     /gene="ompR"
                     /locus_tag="ACHKUB_000786"
     CDS             complement(36396..37115)
                     /gene="ompR"
                     /locus_tag="ACHKUB_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO6653843.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37343..37819
                     /gene="greB"
                     /locus_tag="ACHKUB_000787"
     CDS             37343..37819
                     /gene="greB"
                     /locus_tag="ACHKUB_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO6653844.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37916..40237
                     /gene="yhgF"
                     /locus_tag="ACHKUB_000788"
     CDS             37916..40237
                     /gene="yhgF"
                     /locus_tag="ACHKUB_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO6653845.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40675..40902
                     /gene="feoA"
                     /locus_tag="ACHKUB_000789"
     CDS             40675..40902
                     /gene="feoA"
                     /locus_tag="ACHKUB_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO6653846.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40919..43240
                     /gene="feoB"
                     /locus_tag="ACHKUB_000790"
     CDS             40919..43240
                     /gene="feoB"
                     /locus_tag="ACHKUB_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO6653847.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43240..43476
                     /gene="feoC"
                     /locus_tag="ACHKUB_000791"
     CDS             43240..43476
                     /gene="feoC"
                     /locus_tag="ACHKUB_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO6653848.1"
                     /translation="MASLIQVRDLLALRGRMKAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43679..44557
                     /gene="rpnA"
                     /locus_tag="ACHKUB_000792"
     CDS             43679..44557
                     /gene="rpnA"
                     /locus_tag="ACHKUB_000792"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO6653849.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGQVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44586..45356)
                     /gene="bioH"
                     /locus_tag="ACHKUB_000793"
     CDS             complement(44586..45356)
                     /gene="bioH"
                     /locus_tag="ACHKUB_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO6653850.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45394..46077
                     /gene="gntX"
                     /locus_tag="ACHKUB_000794"
     CDS             45394..46077
                     /gene="gntX"
                     /locus_tag="ACHKUB_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO6653851.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSKAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46136..46711
                     /gene="nfuA"
                     /locus_tag="ACHKUB_000795"
     CDS             46136..46711
                     /gene="nfuA"
                     /locus_tag="ACHKUB_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO6653852.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47072..48388
                     /gene="gntT"
                     /locus_tag="ACHKUB_000796"
     CDS             47072..48388
                     /gene="gntT"
                     /locus_tag="ACHKUB_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6653853.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48433..50517)
                     /gene="malQ"
                     /locus_tag="ACHKUB_000797"
     CDS             complement(48433..50517)
                     /gene="malQ"
                     /locus_tag="ACHKUB_000797"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO6653854.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50527..52920)
                     /gene="malP"
                     /locus_tag="ACHKUB_000798"
     CDS             complement(50527..52920)
                     /gene="malP"
                     /locus_tag="ACHKUB_000798"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO6653855.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        12593 a        14656 c        13471 g        12505 t
ORIGIN      
        1 aatgaacaaa tttattaaag ttgcactggt aggtgcagta ctggctacgt taactgcatg
       61 tactggtcat attgaaaacc gtgataagaa ctgctcttac gactacctgc tgcacccggc
      121 aatttctatt tctaaaatca ttggcggttg cggtcctact gcacagtaag tctctgacag
      181 aaatcggcta acaccggttt aattgcccga cgccctctgg attttccgag ggcgtatttg
      241 tttctatgtc cttcttgccc ccgcccgtaa aaatattttc actttaaatt caatttgata
      301 actacatcca ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc ctccacatta
      361 cataacatca tatgttgtta tattcatcat gcattgtcat gttacctttt aaatgactgc
      421 aaactctccc ctacaacgta ttggacaaga aaaaggtatc gctatgggaa gccaggaact
      481 ccaacgcaag ctcggatttt gggccgttct tgcaatcgcc gtcgggacaa ccgtcggctc
      541 cggtattttt gtatctgtgg gtgaagtggc aaaagcagcg ggcacgccgt ggcttacggt
      601 gctcgcgttt gtcattggcg ggttaattgt gatcccgcaa atgtgcgtct atgcggaact
      661 atccaccgct tatccggaaa atggcgcaga ttatgtttat ctgaaaaatg ccggaagccg
      721 accgctggct ttcctctccg gctgggccag cttctgggcc aacgatgcgc cgtcattgtc
      781 gattatggcg ctggcgattg tcagcaatct tggcttttta acgcctatcg atccgttgct
      841 cggtaaattt atcgccgccg gattaattat cgcctttatg ttgctacacc tgcgctccgt
      901 tgaaggcggc gcagcgtttc agacgctaat taccatcgcc aaaattatcc cgttcactat
      961 cgtcattggc cttgggatct tctggtttaa agcggagaat tttgccgccc ctaccaccac
     1021 tgcgattggc gcaacgggca gctttatggc gctgctggcg gggatctctg ccaccagttg
     1081 gtcgtatacc ggcatggcct ctatctgtta tatgaccggc gaaattaaaa accccggaaa
     1141 aaccatgcca cgagcgctga ttggttcctg tctgctggtt ctggtgctct acaccctgct
     1201 ggcgctggtg atttccggcc tgatgccctt cgacaaactc gccaattctg aaacgccgat
     1261 ttccgacgcc ctgacctgga tccccgcact cggcagcacc gctgggatct ttgttgccat
     1321 cacggcgatg atcgtcattc ttggttcgct ttccagctgc gtgatgtacc agccgcggct
     1381 ggaatacgcg atggcgaaag acaacctgtt ctttaaatgc ttcggccatg tgcatccgaa
     1441 atacaacacg ccggatgtct ccatcatcct gcaaggggcg ctggggatct tcttcatctt
     1501 cgtttccgat ctcaccagcc tgctgggtta tttcaccctg gtgatgtgtt tcaaaaatac
     1561 cctcaccttc ggctccatca tctggtgtcg taaacgcgac gattacaaac cgctgtggcg
     1621 tactccggct ttcgggctga tgaccaccct cgccattgcg tcaagcctca ttctggtcgc
     1681 ctcaaccttt gtctgggcac cgattcccgg ccttatctgc gccgtcatcg ttattgctac
     1741 tggtctgcct gcttacgcct tctgggcgaa gcgtagccgc cagctcaacg ctttgtcgta
     1801 atgttatctg gagaaaataa aatgttggat attgataaaa gcaccgtgga ctttctggtc
     1861 accgaaaata tggttcagga agtggaaaaa gttctcagcc atgacgttcc gctggtgcac
     1921 accatcgtgg aagagatggt gaagcgcgac attgatcgta tttatttcgt tgcctgcgga
     1981 tcgccactca acgcggcgca aacggcgaaa catctggcgg atcgcttttc cgatcttcag
     2041 gtctacgcca tttccggctg ggagttctgc gataacaccc cgtatcgcct cgacgatcgt
     2101 tgcgcggtga ttggcgtttc tgactacggt aaaacggaag aggtaatcaa agcgctggag
     2161 ctgggccggg cctgcggcgc actcactgcg gcgttcacca aacgcgcgga cagcccgatt
     2221 acctcggcgg cggaatttag cattgattat caggccgact gtatctggga aattcacctg
     2281 ctgctctgct acagcgtggt gctggagatg atcacccgcc tcgcgccgaa cgcggaaatc
     2341 ggcaagatca aaaacgatct caagcagttg ccgaatgcgc tcggtcaact ggtacgcacc
     2401 tgggaagaaa aaggccgcca gcttggtgaa ctggccagcc agtggccgat gatttatacc
     2461 gttgctgcgg gtccgctgcg tccgctgggt tacaaagaag gcattgtaac gctgatggaa
     2521 tttacctgga cgcacggctg cgtgattgag agcggagagt tccgccatgg cccgctggag
     2581 attgtcgaac cgggcgttcc gttcctgttc ctgctcggca atgatgaaag tcgccacacc
     2641 accgaacgcg ccattaactt tgttaaacag cgtactgaca acgtgatcgt catcgattac
     2701 gccgaaattt cgcaagggct gcacccgtgg ctggcaccgt tcctgatgtt cgtgccaatg
     2761 gagtggctct gctactacct gtctatttac aaagatcaca acccggatga acgccgctat
     2821 tacggtggtc tggtggaata ttaatccctc tcccggcccg gcaacgggcc gaatttacgc
     2881 aaggagttac ccgatgaaaa caggtatgtt tacctgcggc caccagcggc tgcctattga
     2941 acacgcattt cgtgatgcaa gcgagctggg ttacgacggc atcgaaattt ggggcggtcg
     3001 cccgcacgcg ttcgcgccgg acttaaaagc gggcggcatc aaacaaatca aggcgctggc
     3061 gcagacgtat cagatgccga ttatcggcta tacgccagaa accaacggct atccgtataa
     3121 catgatgctg ggcgatgaac atatgcgtcg cgaaagcctc gacatgatca agctggcgat
     3181 ggatatggca aaagagatga acgcgggtta tacgctgatt tccgcggccc acgcgggcta
     3241 tctcacgcca cctaatgtta tctggggacg gctggcagag aacctaagcg aactgtgtga
     3301 gtacgcggaa aacatcggca tggatctaat cctcgaaccg ttaacgccgt atgaatcgaa
     3361 cgtcgtgtgt aatgctaatg atgtgcttca tgcgctggcg ctggtgcctt cgccgcgcct
     3421 gttcagcatg gtcgacattt gcgcgccgta tgtccaggcg gaaccggtga tgagttattt
     3481 cgacaaactg ggcgataaat tacgtcatct gcatattgtc gacagcgacg gggccagcga
     3541 cacgcattac attcctggag aaggcaaaat gccgctgagg gaactgatgc gcgatattat
     3601 cgaccggggc tatgagggtt actgtacggt ggagctggtg acgatgtata tgaacgagcc
     3661 cagactctat gcccgccagg cgctggaacg ctttcgcgcg ctgctgccgg aggatgagag
     3721 atgaaaaccc tggcgacaat cggcgataac tgcgtcgata tctacccgca actgaataaa
     3781 gcgttttctg gcggtaatgc ggtcaatgtg gcggtgtact gcactcgcta cggcatacag
     3841 ccgggatgca ttacctgggt gggtgacgat gactacggca caaagctgaa gcaggatctc
     3901 gcccgcatgg gcgtcgatat cagccatgtc catacgaaac acggcgttac cgcacaaact
     3961 caggtggaac tgcacgacaa tgatcgcgtt tttggcgact acaccgaagg cgtgatggcc
     4021 gactttgccc tgagtgaaga ggattacgcc tggctggcgc agtatgacat tgtgcacgcg
     4081 gcaatctggg gacatgcgga agacgcattc ccacagctgc acgctgcggg caaacttacc
     4141 gctttcgact tctccgacaa gtgggacagc ccgctctggc agacactggt gccgcatctc
     4201 gattttgcct ttgcctccgc accgcaagaa gacgaaacgc tgcgtctgaa gatgaaagcg
     4261 attgttgccc gtggcgcagg aacagtgatt atcacgctgg gtgaaaacgg cagcattgcc
     4321 tgggatggcg cgcagttctg gcgtcaggct cctgaaccgg tgacggttat cgacaccatg
     4381 ggtgccggag attcgttcat tgccggattc ctttgcggct ggtctgcggg gatgacatta
     4441 ccgcaggcga tggcgcaggg aacggcgtgc gcggcgaaaa ccattcagta ccacggtgcc
     4501 tggtaggtat aacgttggcg tgagcatctt cacgccaacg tgctgttact tgccggaaaa
     4561 cgaccctata atccgagtaa ttcattcttt atttcagggt cgattatgtc agctacggac
     4621 cgctactctc atcaactcct ctacgctacc gtccgccagc gactgctgga tgatatcgcg
     4681 cagggggttt accaggccgg gcaacagatc cctaccgaaa acgagctttg tacacaatat
     4741 aacgtcagcc gcattaccat tcgcaaagcc atcagcgact tagtggcaga cggcgtactg
     4801 atccgctggc agggaaaagg cacctttgta caaagccaga aagttgaaaa cgccctgctt
     4861 actgtcagtg gttttaccga ttttggcgtc tcacaaggca aggcgacgaa agagaaagtg
     4921 atcgaacagg aacgggtcag cgccgcgccg ttttgcgaaa agctgaacat ccccggaaac
     4981 agcgaagtgt tccatctctg ccgggtgatg tatctcgata aagagccgct gtttattgat
     5041 agttcatgga tcccgctgtc gcgttatcct gactttgatg agatttacgt cgaaggaagc
     5101 tccacctatc agttatttca ggagcgtttt gacacgcgag tggtcagcga caaaaagacc
     5161 atcgatatct ttgccgccac ccgcccgcag gcaaaatggc tgaaatgcga actgggcgaa
     5221 ccgttgtttc gcatcagcaa aatcgccttt gatcagaatg acaaaccggt gcacgtctcc
     5281 gaactcttct gccgcgccaa tcgcatcacc ttaactattg ataataaaag acattaaccg
     5341 taggccggat aagatgcgcc agcatcgcat ccggcgatgc tggcgcgttg aattttacat
     5401 cccgtacgtt cccctcaccc taaccctctc cccaaagggg cgaggggacc gttcagtacg
     5461 gaatttagct tgacggtgct atccagcatt aaatactggc aatactctca cgcaaaatcc
     5521 gcagcaccgt ctcttcaccg ctgcgattcg ggttaatgcg aatcgcacaa tgttctgatt
     5581 gtggattcgc ctggcgaaac gttccggaaa ggcgataaaa gagcggcggg atttcatatt
     5641 tcgactctgc acccaccggg taaggcaagg caccgcgctt ttgcgcctct tccagcactc
     5701 tggcggcaat cggctgatga aactcgacaa tcaacacctt cgactgcgca ttagcaatca
     5761 ccgcgctttt cacttccggc accgcaccac cgttaagcaa agccagcaac cgttcagata
     5821 cccctgcctg caccgcgtgc atcactggcg caaacaccag accacgcaat acttccagcg
     5881 cctgtgcgcc ctggatctgg ctaccgccgg agtaaagcgt ggcgcgaata cggttgataa
     5941 catcagcatc gccgaccact gcaccaacac cctctggccc aaatagcttg aagcaggaaa
     6001 atgtcgagac attcgcgccg cattcacagc cgattcgcgc caccttcatc accgcatagt
     6061 tgtcatcggt taacgctgga acacctgccg cgcgcaacgt tgccagcaca tctgccagca
     6121 cgtagctgtc ctgcggctgc tggcgcgtat gctgcaccag cgccgcatcc ggttgttgct
     6181 cgtcgacgac ctgcttcagt gccgacaggt cattgaaatc aacagtaata agcgtcagcc
     6241 ccatctgctc aataataacc cgtgtcgtcg ggtaaacagg cgcgtcatgc accagaagac
     6301 gctgccccgg tttgagcaaa gccgccagcc cggcgcgaat cgcgccagtc cccgcgccct
     6361 gcaccagcgc cgcagcctgt gcgtgaaatg catcagccag cacctgttcc acacgctggg
     6421 taacgcgcgg ttgattcagt cctggcgtta agcctaaatc accgccagta agaaactcgc
     6481 tgccggggaa atggcgacaa atgctatcca ccagcgcaaa ctgcttttgc tgcgcctcaa
     6541 taatcgtcag gctttgcaga ggaaacgtct tcataggtgc cttccttaag ccggaacgaa
     6601 caggccaagc cagtaaagaa cattcagcag aataccggtg atcattactg ccacgaccgg
     6661 tgccgccatt ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc
     6721 gatagagaat ccggtataac ccgccatctt aattgctgcg aaaatcgaac cgaccagcag
     6781 cgccacttcc atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgcaccga
     6841 cgggtagcgt cccagccatt tgccgatcga acgaagcagc aagacttccg ccgaaataac
     6901 cactgcgcct aataccgctg caaccatcgg attcggcgag agatagccca ccgcataaac
     6961 aaaggtaaag cccgcaactg catacacacc cgttgctaag gcggtggtgg caatcaacgg
     7021 tacaaaaccc agcccgcgca taaattctgc cagtgccgcc tggttgatca gcgtttgcga
     7081 ttgttccggc gttacgcctg cggagtacgc tttctccaga gtgaagatcg acacttcact
     7141 gccggcgaaa atcttcatgc tggcaacggc ggcaatcaat gctcccacga tggcgatata
     7201 gggtaagttt ttgataatcc gcgacgtgcg ttcttcaaac accgaaagcc cgctggcatc
     7261 aatatcattt tcatcacgat gacgcaggtc gtgagttatc gcgatcccca gcagcatcac
     7321 catgccaata aagatttcga tggattcagg gttaagatgt gggaaatagc gcacgactac
     7381 cacacgggtc atcagtacca ccacggcggc gatcagactt tgcttccagc caaactggta
     7441 gaaaatcgcc accagcggga acagtgcaaa agctgaaacc accggcgagc ttaattcccc
     7501 caggctacct aatacatcca ccggcagcgc ggtcagcagc tggtttactg gcaacaggca
     7561 agtaaggatc aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc
     7621 caggacgccg aggatatcgg taggaagaaa gagtaaccat gcgttgagca gcccggtttt
     7681 cagggtaaaa gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatact
     7741 ccccgcctcg cgacggttca tatagccttc aatcagttgc ggcaggatcg ggcggatgcc
     7801 gtcatgaaaa acagccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc
     7861 caccacgata atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc
     7921 aacatcggaa tcgcgtgttc gacgtgctca acggaaaggc caaacgccac ttttccttcg
     7981 gcgatcattt tggcgatatg ctcgtctttc gctttaatgc ctggtttggc aatggtgcaa
     8041 cttttgttat agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta
     8101 cacgcgccga tgtaataatc cagttgcccg gatttcactt tcattgccgc ttccatgtcg
     8161 ttgtgaatga aaacttcaaa actgccagga gccgtcgctt caatggtttt tttaatctgc
     8221 tcacgctgta agcctgcaac gccaatcttt ttcatgagtc tgtccttatt ggaaaaattg
     8281 agagggagtt tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa
     8341 ttgcggaata aaggtggtca ataaatagtc atagccataa ccaccattgg cttttaaatg
     8401 ggagcggcgc gtaatatcca tcgacagcat gacgcggttc agcaacccac ggtcacgtag
     8461 cgcatgaagc atcgcaatac gcttttcgtc cgggtagtaa ctgttcttac caatggtgtc
     8521 gaactgcacg tacgcgccga gatcgatcat cttcaaaatg ttgtcgaggt tgtctttcag
     8581 atcgcagtga ccaacggtga cgcgcgaaag atcaaccccg tgggcttgta gcaacgccag
     8641 ttgctccagc cccatcgtgc tgaacgacgt atgcgtggag atcgggcgtc cggtctggtt
     8701 atgcgccagc gcagcggcaa taaatacctt ctcttccagc ggcgtaatct ttccttcgct
     8761 ggtgccgatc tccgcgatga tcccggcttt cagctccgtg ccatcgatac cctgttcaat
     8821 ttcatcgacc atctcctgcg ccagttcctg cacgctgcgg gtcgccacat gttccgggaa
     8881 aaacgcgtcc tggtaataac cggtacaggc caccacgttg atccccgtct cgcgcattac
     8941 atcaagcata aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg
     9001 cacgccccgg gtcatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg
     9061 gcagtccacg ttgtttttaa agccggagag atcaatatgc agatgctcat gggcgagggt
     9121 gtaacccgtc ggatcaaaac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa
     9181 gagcgaccat tttgtggcgg tggcgcacgg aaaaattcac acacggtagc ccccacatca
     9241 gaaagcgtgg tgcgaacacc tagctgagta gcagccaggc cttgctgata aaccagcact
     9301 ggcaccactt cgcgggtatg gtggctgtga ccaatggtcg gatcgttacc gtgatccgcc
     9361 atgacgatca ggcagtcatc tggctgcatc gcctcaacaa gccgggcaag gttacggtca
     9421 acgacctgca aacgttcggc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt
     9481 tcctggatat tggtgcaaat aaacgccgtc ggatgcgtgt taaattcgtc gagggtgata
     9541 tccataatcc gctggctatc caccaggttt tgccagctca cgccataagg attgctgacg
     9601 atatctgcca ccttacccac cagcacggtt ggcacgcccg cctcatacag tttttgcggc
     9661 acctgcactt tttcatcaac accgtagccc atatgcacga cctggaaacc gttgtcataa
     9721 gcgccagaac gcggcgcatt aataccaata aagcgccctt ctttgctttc tgcggcatcg
     9781 agaatacgct gactgttggt taacaggccg ccaaatgtaa tgacccgacc gacctgtacc
     9841 tgctcacgca cgatacgacc aattttgatt gcgtcgtcaa aagagatcac agagagattg
     9901 gcggtgatgt tatagacctg gcctaaatcc gcctcgagat tatcgccaat cgcaaccgcc
     9961 tgattgaccc acagaaattg cagatcatcg ccacggcgct ccacctgcca accggcggat
    10021 actaatgcct gctcaacacg gtcaatcaca tcgcgaaaag gcatccgcag cggcggtaac
    10081 gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc
    10141 tctgccacgc cccaggttgc ggagtctgac ggctgcatat cgcctggcgc ataacccaat
    10201 gtgttgatta gccccagctt ctccagcgtt ggtagctgca aatgcggcaa ctggctcagg
    10261 atgtgaccac aggtattcgc cccggcatcc tgcgggcgca ccagcgtgac atctttcatt
    10321 gcccctacgc caaagctatc aatcactaac accacaaatc gcgccattac gcccccagag
    10381 agtttccaag actgtcataa cgaccgacga tttccggttc gccacgatga ataccagaca
    10441 ccagcaccac atcactgcgg gtgacaaaaa tctgcgtgcg aaaacagagc actactgcac
    10501 tgcttaccgg aaactcgcct gccagcggca gggtgtagtc gatactgctg tcatccacag
    10561 ttttgagatt ggtttcagta atcttttgat tttctggcgt aaacaccagc gcatgttgcg
    10621 cgtgaccacg acggtagtaa ccgccgccgt agcagtagct gtcgccacgg aaaaaatggg
    10681 agatttcact taaccagagc atcgcgatac gttcaggctg atcgccctgc tggtttgccg
    10741 gaatagtgcc cgtcagcgca tgaccgggtt cggcatgagt cacaccgtat tgcgccagca
    10801 atggcagcga agtgcagctg gtcgctgaag gcgcgttcag ttgctcaatc gcaataccag
    10861 atttcgccag ttgatcccgt gcttgtacca gcgtgtgaag attcggtgtc ggcaaaactt
    10921 tcccggcagc ctcatcccaa agcaggcaag ggaaatgggt tagtcccgcc aaatgcagcc
    10981 ccggcagttt cttaatttca gcaaccacct cgtgcagcga atgctgtaca aaaccgctct
    11041 cctgccccgg ataaagaaaa tcatcgtcgc tataaacttt gagcagcaca gactggattc
    11101 gcccggcctt caccgccgcc gcagaaactt cccgcgcttt gtcgagagta aacacggtga
    11161 tgacgtcggt gccctgttca acggcgtcag caacctgatg acaagggatt tgtaccagat
    11221 gcccctgatg cgccaccggc aaaccagcgc ggcgcatgac tcgcgcctct ttgtaatcca
    11281 ccgccacaat gccgctgtag cccaacgcca gcagtttttc cgccagccat gggttacgac
    11341 cgaattgttt ggtcatcaga tacagttcaa tgccgtaaag ccgcgccgtc tcaatcagcc
    11401 gcttaccgtt ttccagtatc tgatccacgt cgatcaccca gctgtccggg gcgattttgc
    11461 cctgctgcca caaacttagt gcggcggaaa tcagcgccgg gttctggcgt ttcaatgctt
    11521 ctacaaacat cttgttgcct cgtttctgtt ccttgaatta aatattcatt tttttgaata
    11581 tttaggttgt gcgacgtgga tttgtcagga gcttcaaacc tcttcgttag cagccatcca
    11641 taatccataa aggttggcca gcaaatagcc ttcttcacac gggttaactt ccagtgcgaa
    11701 ttccttcaac aacacctgat gcaattgcac aacggcttgc cagtggctgg attgcgccag
    11761 ctctgccagc aattcgttat ccagcggctc tatttcttca ccacggcgac tgcgcatcag
    11821 tgcactcgcc atatgtgtca tcgccatcgt gccttgctca ctgcgcaccg gcagatggca
    11881 ctctgtttcc agtacgttga cgacctgcat catgcctttg cagatgtcct tatcaataac
    11941 gcctgcctcg caaagcaggt tgagtctggt ttccatatca gatgcctttc gtttaataac
    12001 tcggttcctg ttccccactc actacccgtt gttgatgggc gagcagggcg tgtttatcta
    12061 caacggcacg cagcagttgt tgcattgggt aatcatcgac tcgcgtcagg accacggctt
    12121 cggtggcctg taaaaatcgc ggatcgtctg tgagcggcgt cgcctctaat cccagcatgg
    12181 tcagttcgtt ttccgccacc acgttccaga tcaccgcatc gacatcgcct ttaacaatgc
    12241 gttgtaaact ctcgtgataa gagagatcga ctcgttccac atcactaccg ccaaaaaaaa
    12301 catcggtcat gattttctga tccgccgaac ggttatccag ccccacgcgc ttcacgtttg
    12361 cggactcgcc tttacggcaa atcaactggt gctcgccaac gtaggtgtgc ggccccaact
    12421 ccagcgcgag gcataaccct ttttgcgtga gataactttc cgccgccagt cgggaaacca
    12481 ccgccatgtc atacacgccg ttaagtaaac actccacgcg aatatccgcg ccgcgcatat
    12541 gcgcatagta aaaaggaatg ccatcaaact gagctttcag cccgctcgct aaaccttcgt
    12601 acaagcgggt atagggcaaa ggcatcgcac ataccacgtt gttgatatcc acatgactca
    12661 gcaatgcttt gttatccatc tcgaccagat aactgccatt gcgcccacgg cgttcaatcc
    12721 gtatcgctcc gcttgattcc agcgttttca acgcggcctg cgtcaggcca accgatgaac
    12781 ggcattcagt tgccagctca tctatggttt tcaacctatt accgcatttt tcacccaaca
    12841 aataacgggc cagcgtcgtg ataacgacgc cttctttttt gataaacgtt cgacgcataa
    12901 tatattttca ataaaatgaa tgtttatatc ttcattaaaa tgaagataag tggcaactgt
    12961 gagatgtgga aacggcgagg cgcttcacat tttttcggat tattgcaaat gaggataaaa
    13021 aaaccgggtt tccccggttt cagagtgatg acgaagtgat ttattcgaca atgcgagcaa
    13081 agggtaattc ccactggcgc ttgcctgctc ccctcaccct aaccctctcc ccaaaggggc
    13141 gaggggaccg tccgagcgtt ttgatattac ggcttcgcca caaaaccaat cgcttcgtac
    13201 accgctttca gcgtacggga agcgtgtaca ctggcttttt ctgcgccgtc tttcatcacc
    13261 tgttgcagga aggcttcatc gttgcggaaa cgatgatagc gttcctgcaa ttcagtcagc
    13321 ataccggaaa cggcatcagc cacttcacct ttcagatgac catacatctt gccttcgaac
    13381 tgtttttcca gttctgggat gctctggccc gttaccgcag aaaggatatc cagcaggttg
    13441 gaaacgcccg ctttgttctg cacatcgtag cgaactaccg gcggctcgtc ggagtcagtg
    13501 accgcgcgtt tgattttctt cactaccgat ttcggatctt ccagcaggcc gataacgtta
    13561 ttgcggttat cgtcagactt ggacatcttc ttggtcggct ccagcagcga cattacgcgc
    13621 gcgccagatt tcggaataaa cggctccggc accttaaaga tctcgccata cagcgcgttg
    13681 aaacgctggg cgatatcgcg actcagttcc aggtgctgtt tctggtcttc acccaccggt
    13741 accagattag tttgatacag caggatgtcc gcagccatca gcaccggata gtcaaacaga
    13801 ccagcgttga tgttctcggc ataacgcgca gatttatctt taaactgggt catgcggctc
    13861 agttcgccga agtaggtata gcagttcagt gcccagccta actgcgcatg ttctggtacg
    13921 tgggactgaa caaaaatggt gcttttctca ggatcgatac cacaagccag atacaaggcc
    13981 agcgtatcca gcgtcgcttt acgcagcttc tgtgcatcct ggcgcacggt gatcgcgtgt
    14041 tggtcaacga tacagtaaat gcaatggtag tcatcctgca tgtttaccca ctgacgcagc
    14101 gcacccatgt agttaccaat ggtcaattca cctgagggct gtgcgccact aaaaacgatg
    14161 ggcttagtca tttttcgatt cctgattttc gctatgcgga agccctaatg cgggcagaag
    14221 gtcatttata gactgataaa ttacatcagg ctggctgaga tcgatagcct cgccgtagtt
    14281 atatccgtag gttaagccaa ctgatgggca acctgccgct tttgccgcct gaatatcatt
    14341 gcgtgagtcg ccgacaaaca gcatctcttg tggggcaatt cccatccgct cagccaccag
    14401 taacagcggg tccggatgcg gttttttgtt ttgcacatca tcgccgccga taaccacgct
    14461 aaagtatttg gcaatatcta aggcttcgag cagcggcgcg acgaacggcg tcggtttgtt
    14521 ggtgaccagg cctagcggca ggcctttagc ctgcaacgcg cccaacgtat cggcaacgtg
    14581 cgggaacaaa aacgtcccct cttcggcaac ctcgctatag tagcgatcga acagtttacg
    14641 cagaatacgt acctgttctt ctgccggaat gtcgtcatca acgggcggtt tacccattgt
    14701 tttacgcaga gtcgcacgtt cctgacgcgc ccaggccaat gcacgctcca tcagaacatc
    14761 tgcgccgtta ccaatccagg taataacgcg ttcttcacct gcgacgggta actccaacgc
    14821 atacagcgcc atatctaccg cagcagcaag accgggagca ctgtccacca gcgtaccgtc
    14881 gagatcaaaa gcgacgccgc gaatatcttc aaacttactc atgacttacc tttgccagtt
    14941 cactgcgcat ttcatcaatg acttttttat agtctggctg gtcgaagatt gccgaaccgg
    15001 cgacgaacat atccgcgccc gccgcagcga tttcgccaat gttgttcacc ttcacgccac
    15061 cgtccacttc cagacgaatg tcaaagccag actcgtcgat acggcgacgt acttcgcgca
    15121 gtttatccag tgtttgagga atgaaagact gaccgccgaa accaggattg acggacatca
    15181 gcaggatcac atccagctta tccatcacgt aatccagata gctcagaggt gtcgccgggt
    15241 taaataccag acccgcttta cagccatttt ctttaatcag ttgcagcgtg cggtcaacat
    15301 gctcggaggc ttctggatga aaggtaatga tgctggcacc agcggcagcg aaatcaggca
    15361 caatgcgatc gacgggtttc accatcagat gtacgtcgat aggggcggta atgccatagt
    15421 tacgcaagga tttcagcacc attggcccaa tcgtcagatt gggaacatag tggttatcca
    15481 tgacgtcaaa atgcacgaca tcagcgccag ctgccagggc ttttgcggta tcttcaccca
    15541 ggcgggcaaa atcagccgac agaattgagg gggcaatcaa atactgtttc atccgcttct
    15601 ccttgagaat tattttttcg cgggtgaaac gactcctggt ttgtacaaag ccagcagttc
    15661 gtccaccttt ttacgtgtgc cgccgttgct gcttatactg cgtcgaactt tgacgacatg
    15721 caattttgcg cgctgatacc actcacgcgt taacatcgta tcgtgattgg agatcagcac
    15781 tggaatatgg cgctcaacca gaccttcggc gatctccgcc agatgcgctt gttgttcaag
    15841 cgtaaaactg tttgtgtgat acgccgtaaa gttggcggtc gcagacagcg gtgcataagg
    15901 cggatcgcaa tagacgacgg atgcatcatc tgcgcgcgcc atgctatcgg cgtaagactc
    15961 acaatagaaa aaggcattct gcgctttttc agcgaagtga tacaactctg cttccgggaa
    16021 atagggtttt ttgtagcggc caaacggcac attaaactca ccgcgcagat tgtaacgaca
    16081 caggccgttg taaccgtagc ggttcaaata taaaaacagt accgcccgac ggaacggatc
    16141 ctggcttttg ttgaactctt cgcggaactg atagtaaacc tcggcgcaat ttgtttcggg
    16201 aacaaacagc tcgcgtgcgg cctgtacgta ctcatcagta cgcatcttca caatgttata
    16261 gagactgatc aggtcgctat tgatatcggc aaggatataa cgagaaaagt cggtgttgag
    16321 aaacaccgac ccggcaccta caaaaggctc aaccagacat tcgcccttgg gcaaatgccg
    16381 tttaatatca tcaagcaggg gatacttgcc ccctgcccac ttcaaaaaag cgcgattttt
    16441 cttcatgctg actaactaat tacaccttct ccggctgtgg agaaagctcc gacagcatcc
    16501 agcgcttcag gcaactcccg caacgataaa ccattgcggg agataacctt gattacttca
    16561 gatcggcctg tacctgacgc agcggtttcg cccacgggtt tttggcctgg acatctgctg
    16621 gcaatgtaga taccgctttt ttcgcctctt ctttcgaagc gtacacgcca gaaaccagga
    16681 cataccacgg ctgaccatta cgcgtcgttt catagacaac gtagtttttc agattctctt
    16741 tcttcgccca accgttcagg ttgtcgtagt tagaggaact gctcagctgc agagtgtaat
    16801 ggctggacgg tgccgatttc aacgaaccaa cattacctgc ggtcttccct ccagcggctg
    16861 gtgttgccgt ggtttgcgcc ggggatgccg tctgtactgg agccgtagtc gccgtctctt
    16921 ttggtgctgg cgtagaagtc gcagccggtg ccttcgtgct cgccacaggg gcagctggtt
    16981 cagtacgctt cggcgtctgt gctaccggct tcggctccgt tttcacggtt gcttgcggtt
    17041 ttttcggttc aatcactgcc tgctggcgag ctggacgcgt agtggccgga cgttcagcgg
    17101 tttgcgtttt cgccgtgtca cgcgatgcat tgccattgcg aacaggcgcg acagtcgcgg
    17161 gttcggtcgg caatgtggaa ttgaccgcca cattgttcaa ctgttgctga ttttgtggct
    17221 gggtcagcgc attgttcagg tcaccctgca cttcaacacg ttgttgacca tccgttgccg
    17281 ccggggtttg cccttgagtc ggcgtagaag agatcggtgg cagagaaaca tcctgctgag
    17341 tattttccgc agacgtggtt cccggcgctg gctgcacgcc attcgcctga tcggtcgcat
    17401 tgcctgcaag atcaatactc ttctcgccag acgcggtttg atcgctggaa gaggtcgagg
    17461 gggcttttag cgcagaaccg ataccgatga tcaacagcag tagaaccaga atgccgacac
    17521 ccatcatcat atactgacga gaagcgggtt tactggctgc ttttttgcgc ttacgcggac
    17581 gacgctctac gcgctcttca tccacggttt catcttcgga ttcgtcaatt tctgcttcga
    17641 tttccggttc ctcattacgc tctttttgcg cacgagtcgg acggcgatcg tcagtgtcat
    17701 caagttcaat atcatcaaaa ttgatctgcg gttcgccacg ttcagtacgc tcagaacgtt
    17761 cagaagattg acgagaacga ccagtacgac gatcgctggg atcgggtttc agctcgtctt
    17821 ctggtttgaa ttcatccatt taacacccca ctaaaaggtt aatgcttacc acgttgcaat
    17881 taacctgaag ctaatagacc gcttgataag cggcctgacc tttcttgttg ttacgctgat
    17941 tgacaatcgg caatggcgtt aagaacaagc tcgtgcgaaa cgccgctgcg aacttcactc
    18001 ttaccaattg ccaacggaag aattaagcgt atctctcccg caaggacttt cttgtcacgc
    18061 agcatatgcg gtaaatacgc ctgcgcggac atttcgcgcg gcccattgac cggtaaccca
    18121 gcccgcttga gcagggttat aatacgctgc gtttcggcag aactaaactg cccgagacgt
    18181 tccgacgtcc gcgccgccat caccataccc gcagcgaccg cttcaccatg taaccaattg
    18241 ccatacccca tttcagcttc aatggcatga ccaaaggtgt gtcccagatt cagtaaagca
    18301 cgtaacccgg tttcgcgctc gtcggcggcg acaacttctg ccttcagttc acaacaacgg
    18361 cgaatacagt acgccattgc cggaccgtcc agacgcaaca acgcatccag attctcttcc
    18421 agccagttaa aaaacgcacc gtcaagaata atgccgtatt tgatgacttc tgccagcccc
    18481 gacgctaact cacgcggggg aagcgttttc agacagtcga gatccaccac cactgaagca
    18541 ggctggtaga acgcgccaat catgttttta ccgaggggat ggttgaccgc agttttgccg
    18601 ccaacggagg aatcgacctg cgacagtaac gtcgtcggga cttgaatgaa acgaacaccg
    18661 cgctgataac tcgccgccgc gaagccggtc agatcgccca ctacaccgcc gccaagcgcc
    18721 accagcgtag tatcgcgacc atgcggcttt tgtaacaacg ccgtaaagac ggtatcgagt
    18781 acagccaggc ttttatactg ctcgccgtca gggaggataa cgctatcgac gttaacaccc
    18841 gcctgttcaa gtacgccgcg gaccttatcg agatacagag gagccagggt ttcgttggtg
    18901 accaacatga cctgctcgcc cgatttcagc ggtaagaatg aagctggttc attaaacaaa
    18961 ccagatgcga tggtaattgg gtaactacgt tccccgagag taacgacaat cctctccata
    19021 acgcgacatc caccttaatt actgtacccg cagacgagtg tatataaagc cagaattagt
    19081 tgctttccag catgtgaata atctggtttg caaccacttt agcgctttga tcatcagtac
    19141 gaatggtcac gtcggcaatc tcttcataca gcggattgcg ttcattggcc aacgcttcca
    19201 gaacttcacg cggcggtgtt tcaacgtgca gcaacgggcg ttttttatca cgctgcgtgc
    19261 gtgcaagttg cttttcgatg gtcgtttcaa gataaacgac aacgccacga gcggaaagac
    19321 ggttacgcgt ttcacgggat ttcacagagc cgccgccagt agccagcaca ataccctgtt
    19381 tctcggtcaa ctcattgatg accttttctt cgcgatcgcg gaagccttct tcgccttcta
    19441 aatcgaaaac ccagcccaca tcagctccgg ttcgtttctc aatctcttga tcggaatcgt
    19501 aaaattccat attgagttgt tgagctaact ggcgcccaat agtgcttttt ccggcaccca
    19561 taggcccaac cagaaagata ttgcgtttct ctgccatttt ttcggtacta ctaagactat
    19621 tcgttaatga taaacccgct tcgctcagag agcgccgcag gacatgaact gaaacctcat
    19681 aagatattgc gagagtcaga ctgaaaatta tctcaatact ccagcgggtt tggcaactga
    19741 ataaatcacc catccctctg catatctggt cgctgcaagc gcgctgcctt gctaccaccg
    19801 ctctggcgat aaatcaccgg gtaagattag cgtaaaaaag acagcaaaat gccgcctgaa
    19861 tgataaatca tcatcatgga acgcaggcag cgaagagcac caactctcaa atcggtactc
    19921 cttgtatgct aaatacctgc gcgcgtcaaa tagatgaaac acgttcagca taaaaacatt
    19981 accgctttta cggctgttta ctcactggaa accagtcgtg gcgtgataaa caccactaac
    20041 tcgcgtcgtt catcttcttt tccgtcatga cgaaataatt gcccgaacca gggaatgtcg
    20101 ccaagcaacg gtacgctatc ctgacccgat ttatttttac gggtaaaaat gccgcccagc
    20161 gccaacgttt ctccgctttt gacctcgacc tgcgtttcga tctcctgctt atcaatcgcc
    20221 agcacttcgc catcggcctg ctgtagcacc tgccccggaa cgttctggct gatgtgtaat
    20281 ttcagccgga tgcgaccttt ttgtaacacc gtgggcgtga cctccatccc caggacggcc
    20341 tctttaaatt ccaccgacgt cgcgccactt tccccgctgg aaacctgata tggaatttcg
    20401 ctcccctgtt taatgctggc aggctgaaga tgtgaggcca gcagacgcgg actggcgata
    20461 atatccagct gctgtttttg ttcgagcgcg gaaagctcaa gatccagcaa gcgtccgttg
    20521 atgcgcccaa tgttaaaacc gacatgcgtt gtcgccgtcg ctacggagag gtcgctacca
    20581 agcgtggtga cttgcccaac gccaccagcg tgttgcgcat cggccagcgt ccatttcacg
    20641 cctaactcac gcaaactttt ttcattaatg gtgacaatat gcgccgacag ctcaacctgc
    20701 ccgaccggca gatccatttg cgctacccac tgttcaagcg cgcttaacgc cgttttgtta
    20761 tctcgtagca aaagacgatt ggtgcgttta tcgacggtca tactcccttt ggcactcagt
    20821 agcttctccc ccgctttcgc cagttctccc gcgtcggcgt attgcagggt tatactgcga
    20881 ttttccagcg gcagatttgc ctgcgcccgc gcctgctccg cctcctggcg ggcgatattg
    20941 ttattctgcc aggcaatgga atgcactgag agaatgttgc cttcctgccg cgttatcagt
    21001 ccggcgcttt tcactacagt ttgtagtgcc tgcttccagg gaacatctgt tagatgtaac
    21061 gacaccgtac cgctgacgtc tggcgacacg accaggttca acttctcctg ttcagccagc
    21121 gcctgcaaca ccgagctacc ggaacgtcat ccaccatcag cgtcactttt tgcggctttg
    21181 ccgcctgtac gccgggtatc agcatcaaca gtagtgcggc tatccattgc ttcatttgta
    21241 tctccttgcc gttgccacaa ccattgtggc ggttcgcagt ttgtcccggt tcccagcgtt
    21301 agtgcgtctg gcgtcagctg taaaattgtc cagccgtttt ccagcacatc gttttgctgc
    21361 acccgtcgcc atttcttttg cccgtctttt attacaccga tgatgtgctc gcctcgccct
    21421 accatcccct gatagcgcca ctggctaagt tcgctaatcc ggcatagatc ttccggcggt
    21481 ttaaaagggt cacgcatacc ggttaaaagg cacaatgcaa tacctgccaa caaccagcgt
    21541 ttaaccctca tgcggtgtct ccagttgtag cgtgaacaaa agatcatcac cttccacgct
    21601 taacgaaaaa cggctcacgc tgacgttgcg ctctgccagc cgtgtaaatg ccgacggcac
    21661 tgcttcccac agcgttttca acgccaactc acctccctgc gcggatggat gccaggaaac
    21721 cagttgcgcg ccggataact gaaaatccag aggcgaaaag ggcagcgttt tttcctcgct
    21781 gaagggagtg gtgtctacca ggcgatacag tgcggcccac tgctggtgat gacttgcccg
    21841 tagtcgaatt aatgcgtcgc gctcttcatg gtgtgtcgat gatagaaaaa tgagcgtaac
    21901 taacatcagc aaccagaatg cccagcaaag ctggcggagg cggggtgatg tggcgaacca
    21961 ccagtcaaag aacatgttca tcgctaacct tccttgttaa ctgatactca aattgccagc
    22021 gtccctgcgc atcctgctgc gtggctcccc gctgattgag atgaaaagac gcatcctggc
    22081 ggagtgacgt ttctagtgcg tttaacgcgg taatgcttgt tgtcagcccc ttgatctcca
    22141 gcgttccctg ctgccagctt atcgttgtca gccaggcgtg ctcgggtaaa agcgccgcca
    22201 gtgcttccag cgcagattgc cagtcgcggg taaattgtcg ctggcgctgc cgttgcgaac
    22261 gctgttcgcg taactgctgt tgctccagca aacgtggctt cgttatctgt aagctgcggg
    22321 cgagttgttg ttccgcctga agcaaaactg cgtctatgcg cgcttcggcg ctgcctgtca
    22381 gacgcagtat tagcgttatc ccgacggcca gcagcagagg cgcaacgaac atcagcaacc
    22441 agaaacgcag aaaagcggtc cggcgttgct gtcgccaggg caaaaaatta attggcgggt
    22501 tcatcagtac gccttcccaa gcgccagccc cagcgcgatg gcaaagtctc cacctgacgg
    22561 cggtagcggc ggctgacgaa cagaaacggc ctcccaggga tcaaatcccc cttcgccaca
    22621 tatcgcgacg ctttcgggat caacggataa cgctgccgcc agctccttcg cgctagtcat
    22681 ccccaccgcc agtttgcgcc cccagcgata gcgtgtcgcc cacagccact gttcgttatc
    22741 acgccaggcc agacattgct gatgagaagg taaaaaaggc aggaatcgct gtaatgcact
    22801 ggcatccggg gtgatcgcac taacatgaac acgcaacctt tctgccagag taagcagcgt
    22861 tgccagctct ttgctttgcg cggcagtcac gttataagcg gggctgagtg agtcttcgct
    22921 ataatcgaag cgcagggagt ccggatccat atccagctcg cgggccatcg tccctgacag
    22981 ccaggccgtt tgctcccgct caccaaggga catcgacggg cgcggaaatg accgctgtaa
    23041 tgtgcgactg gcgggaaacg ccaacataat gtgatgacgc tgcggcagtt cgcgactcca
    23101 aggtaacaac gttttagcca gctgctgcgc atcaacaatc cgcccatctt tgataatgtc
    23161 gttctccagc ggcaaccgcc accagcgttg caaaaagcat tcttttgcgc cccgtacgat
    23221 cgcaaccgct accgcttctt gctgttgtaa atgcaaacca atttgccaga tcttaaatgc
    23281 cattgtgatg atctccttat cacccgtcac tctgacgggt atatcaatgc gtctggcttg
    23341 cctttatact accgcgcgtt tgtttataaa ctgcccaaat gaaactaaat gggaaatttc
    23401 cagtgaagtt cgtaaagtat tttttgatcc ttgcagtctg ttgcattttg ctgggagcag
    23461 gctcgattta tggcctatac cgctacatcg agccacaact gccggatgtg gcgacattaa
    23521 aagatgttcg cctgcaaatt ccgatgcaga tttacagcgc cgatggcgag ctgattgctc
    23581 aatacggtga gaaacgtcgt attccggtta cgttggatca aatcccaccg gagatggtga
    23641 aagcctttat cgcgacggaa gacagccgct tctacgagca tcacggcgtt gacccggtgg
    23701 ggatcttccg tgcggcaagt gtggcgctgt tctccggcca cgcgtcacaa ggggcgagta
    23761 ccattaccca gcagctggcg agaaatttct tcctcagtcc agaacgcacg ctgatgcgta
    23821 agattaagga agtcttcctc gcgattcgca ttgaacagtt gctgacgaaa gacgagatcc
    23881 tcgagcttta tctgaacaag atttacctcg gttaccgcgc ctatggtgtc ggtgctgcgg
    23941 cacaagtcta tttcggtaaa acggtcgacc aactgacgct gaacgaaatg gctgtgatcg
    24001 ccgggctgcc gaaagcgcct tccaccttta atccgctcta ctcaatggat cgtgccgtcg
    24061 cgcggcgtaa cgtcgtactg tcgcggatgc tggatgaagg gtatatcacc caacaacagt
    24121 tcgatcagac acgcactgag gcgattaacg ctaactatca cgcgccggag attgctttct
    24181 ctgcgccgta cctgagcgaa atggtgcgcc aggagatgta taaccgttat ggcgaaagtg
    24241 cctatgaaga cggttatcgc atttacacca ccatcacccg caaagtgcag caggccgcac
    24301 agcaggcggt acgtaataac gtgctggact acgacatgcg ccacggctat cgcggcccgg
    24361 caaatgtgct gtggaaagtg ggcgagtcgg cgtgggataa caacaagatt accgatacgc
    24421 tgaaggcgct gccaacctat ggtccgctgc tgcctgccgc agtcaccagc gccaatcctc
    24481 agcaagcgac ggcgatgctg gcggacgggt cgaccgtcgc attgagtatg gaaggcgttc
    24541 gctgggcgcg tccttaccgt tcggatactc agcaaggacc gacgccgcgt aaagtgaccg
    24601 atgttctgca aacgggtcag caaatctggg ttcgtcaggt tggcgatgca tggtggctgg
    24661 cacaagtgcc ggaagtgaac tcggcgctgg tgtcgatcaa tccgcaaaac ggtgccgtta
    24721 tggcgctggt cggtggcttt gatttcaatc agagcaagtt taaccgcgcc acccaggcac
    24781 tgcgtcaggt gggttccaac atcaaaccgt tcctctacac cgcggcgatg gataaaggtc
    24841 tgacgctggc aagtatgttg aacgatgtgc caatttctcg ctgggatgca ggtgccggtt
    24901 ctgactggca gccgaagaac tcaccaccgc agtatgctgg tccaattcgc ttacgtcagg
    24961 ggctgggtca gtcgaaaaac gtggtgatgg tacgcgcaat gcgggcgatg ggcgtcgact
    25021 acgctgcaga atatctgcaa cgcttcggct tcccggcgca aaacattgtc cacaccgaat
    25081 cgctggcgct gggttcagcg tccttcaccc caatgcaggt ggcgcgcggc tacgcggtca
    25141 tggcgaacgg tggcttcctg gtggacccgt ggtttatcag caaaattgaa aacgatcagg
    25201 gcggcgtgat tttcgaagcg aaaccgaaag tagcctgccc ggaatgcgat attccggtga
    25261 tttacggtga tacgcagaaa tcgaacgtgc tggaaaataa cgatgttgaa gatgtcgcta
    25321 tctcccgcga gcagcagaat gtttctgtac caatgccgca gctggagcag gcaaatcagg
    25381 cgttagtggc gaagactggc gcgcaggagt acgcaccgca cgtcatcaac actccgctgg
    25441 cattcctgat taagagtgct ttgaacacca atatctttgg tgaaccaggc tggcagggta
    25501 ccggctggcg tgcaggtcgt gatttgcagc gtcgcgatat cggcgggaaa accggtacca
    25561 ctaacagttc gaaagatgcg tggttctcgg gttacggtcc gggcgttgtg acctcggtct
    25621 ggattggctt tgatgatcac cgtcgtaatc tcggtcatac aacggcttcc ggagcgatta
    25681 aagatcagat ctcaggttac gaaggcggtg ccaagagtgc ccagcctgca tgggacgctt
    25741 atatgaaagc cgttcttgaa ggtgtgccgg agcagccgct gacgccgcct ccgggtattg
    25801 tgacggtgaa tatcgatcgc agcaccgggc agttagctaa tggtggcaac agccgcgaag
    25861 agtatttcat cgaaggtacg cagccgacac aacaggcggt gcacgaggtg ggaacgacca
    25921 ttatcgataa tggtgaggca caggaattgt tctgattaaa aaggcgcttc ggcgcctttt
    25981 cagttggctg acaaagtgca tttgtttatg ccggatgcgg gtgaacgcgt tatccggcta
    26041 acaaaatcgt gaaaaatcaa tatattgcag gaaacccata agcctgataa gcgttgtgca
    26101 tcaggcaaac ttcacgcatt tacactcgcc cctgcccttt caaccattcg cgcacgagga
    26161 acagcgcgct gacattacgc gcttcgttga agtcagggtc ttccagcaaa tccatcatat
    26221 gcgccagcgg ccagcgcacc tgtggtagcg gctctggctc atcgccttcc agcgattccg
    26281 ggtagagatc ttgcgctacc acgatattca ttttgctgga aaaataagac ggtgccatgc
    26341 tgagcttctt caaaaaagtc agatcgttcg caccaaatcc aacctcttct tttagctcgc
    26401 ggttagcggc ttcgtagacg ctttcacccg gatcaattaa tcctttcgaa aaacctaatt
    26461 cgtaggattc agttcccact gcgtattcgc ggatcaggat caggtgatcg tccacaatcg
    26521 gcacaatcat cactgcttcc cggttggttg gacgcattcg ttcataaaca cgccgcacgc
    26581 cattgctgaa ctccagatcc acgctctcga cggtaaacag tcgggaacgg gctacagttt
    26641 caacattcag aatggtgggt ttttgtaatg atttgctcat cgtgggatct atgctgtgaa
    26701 atcagcggtt attgtgcgat atcggacacg ctttcggcaa tgtgaattgc atgttattta
    26761 catttatgta acttaataaa taatcgtcct caaatcaaat taaaagtcaa taggttgaaa
    26821 taactccagg aatttgctga tattccgcct tcagagggtt tgctatgatc agcggttact
    26881 gtgatgtgct taatgatgct caagttaaac tccacgcttg ccgatagcca accgcagaat
    26941 catgtattgt gtctggtgcg actgaccacg cctgacagac taagtaagat ggggaaagca
    27001 tgagcaccat tgtgattttt ttagctgctt tgctggcctg ctcactactt gcgggatggc
    27061 tgataaaagt gcgatccaga cggcgtcagc tgccctggac caacgccttc gcggatgcgc
    27121 aaacgcgtaa actcacacct gaagaacgta gcgccgttga aaattatctt gagagcctga
    27181 cgcaggtatt acaggtgcct ggcccaacgg gagctagcgc ggcaccgatc tctctggcgc
    27241 tgaatgccga aagcaacaac gtcatgatgc tgacacacgc tatcacgcgt tacggcatct
    27301 ctaccgacga tccgaataaa tggcgttact acctcgattc ggtagaagtc cacctgcccc
    27361 ctttctggga acaatacatc aacgatgaga ataccgttga actgatttat accgattcgc
    27421 tgccgctggt tatttcactc aacggtcata cgctgcagga gtacatgcag gaaacgcgcg
    27481 gctatgcctt gcaacctgtt ccgtccacgc aggcgtcgat tcgcggggaa gagagtgagc
    27541 aaatcgagct actcaatatt cgcaaagaaa cgcatgaaga atatgcgctg agtcgtccgc
    27601 gcgggctgcg tgaagcgttg ctgatcgtcg cctccttcct gatgttcttt ttctgcctga
    27661 ttaccccgga tgtatttgtt ccgtggctgg caggcggcgc attactgctg ctgggcgcag
    27721 gtctgtgggg gctattcgcg cccccggcaa aatcctccct gcgagaaatt cattgtctgc
    27781 gcggtacacc ccgtcgttgg ggattgtttg gcgaaaacga tcaggaacag atcaacaata
    27841 tttcactcgg tattatcgac ctggtctatc ccgcacactg gcagccatac attgctcagg
    27901 atctcggtca acaaaccgat attgatatct atctcgaccg ccacgtagtg cgtcaggggc
    27961 gatatctttc gctgcatgat gaagtaaaaa actttccgtt acagcactgg ctgcgcagta
    28021 cgattatcgc tgcgggctcg ctgctggtgc tgtttatgct gttattctgg atcccgctgg
    28081 atatgccgct gaaattcact ctctcatgga tgaaaggcgc gcagaccatt gaagccacca
    28141 gcgtaaaaca actggctgac gctggcgtgc gggtaggcga tacattgcgt attagcggta
    28201 cgggaatgtg taatattcgc acttccggaa cctggagcgc gaaaaccaat tcaccttttt
    28261 taccgtttga ctgctcgcag atcatctgga atgacgcccg ctcattacca ttaccagaat
    28321 ctgaactggt caacaaagcg acggcattga ctgaagcagt taatcgccag ctgcacccta
    28381 aaccggaaga tgaatctcgc gtcagtgcct cattacgttc agcgattcaa aaatccggca
    28441 tggtattgct tgatgatttt ggcgacattg tactgaagac agcggattta tgttctgcca
    28501 aagatgactg tgtgcgactg aaaaatgcgc tggtcaatct cggcaacagt aaagactggg
    28561 acgcgctggt aaaacgcgcc aacgccggga agctcgatgg cgtgaatgtg ttattacgcc
    28621 cggtgagtgc ggaatcgctg gataacctgg tggcaacctc caccgcgccg tttatcacgc
    28681 atgaaacggc gcgagcggca caatcactaa acagtccggc ccccggcgga ttcctgattg
    28741 tcagcgacga aggcagcgat tttgttgatc agccctggcc ttcggcatca ctttacgact
    28801 acccgccgca agaacagtgg aacgctttcc agaaactggc acaaatgctg atgcatacgc
    28861 cgtttaacgc cgaaggtatc gtcacaaaaa tcttcactga cgccaatggt acgcagcata
    28921 ttggccttca tccgatcccg gatcgttccg gcctgtggcg ctatctcagc accacattgc
    28981 tgctactgac gatgctgggt agcgccattt acaatggcgt acaggcctgg cgtcgttacc
    29041 agcgtcatcg cactcgcatg atggagattc aggcctatta tgaaagctgc ctgaacccgc
    29101 aactgatcac cccttcagaa agccttatcg aataacacgt ttgcgcggca ggttatgcta
    29161 ccctgtcgcg caaattgctt cactctggag atttccctca tgcatatcaa cattgcctgg
    29221 caggacgtag ataccgttct gctggatatg gacggcacat tgctcgacct cgccttcgat
    29281 aactatttct ggcaaaagct ggtgcctgaa acatggggcg cgaaaaacgg ggttacgcca
    29341 caggaagcga tggaatatat gcgccagcaa tatcacgacg tacagcatac gctaaactgg
    29401 tactgtcttg attactggag tgagcaactg ggtctggata tctgtgcgat gaccaccgag
    29461 atgggaccgc gtgccgtact gcgtgaagat accattccgt ttcttgaggc actgaaagcc
    29521 agcggtaagc agcgaatttt gctcaccaat gcgcatccgc acaacctggc ggtaaaactt
    29581 gagcataccg gtctggacgc acaccttgat ttattacttt ccacccacac atttggttat
    29641 ccgaaagagg atcagcggtt atggcatgcg gtggccgaag ctacgggtct gaaagctgaa
    29701 agaacgctgt ttattgatga cagcgaagcg attctcgatg ctgccgcgca atttggtatt
    29761 cgttactgcc tcggcgtgac taatcctgat tccgggattg ccgagaaaca gtatcaacgc
    29821 catccgtcac tgaatgacta ccgccgcctg atcccctcgc taatgtgaag gagacgccat
    29881 gaaagaaaaa cctgctgttg aggttcgact ggataaatgg ctatgggctg cccgttttta
    29941 taaaacccgc gcgctggccc gtgaaatgat tgaaggcggt aaggtgcatt acaacgggca
    30001 gcgcagcaag ccgagcaaaa tcgtcgagct gaatgccacg ctcactctgc gccagggaaa
    30061 tgacgaacgc acggtgattg taaaggcgat tactgaacag cgtcgccccg ccagcgaggc
    30121 agccttgctg tatgaagaga ctgcggaaag tgtagagaaa cgcgaaaaaa tggcgatggc
    30181 acgtaaactt aatgccttaa ccatgccgca cccggaccga cgcccggaca aaaaagagcg
    30241 ccgcgacctg ttacgattta aacacggcga cagtgaataa ctgtcacctg caagagagat
    30301 gattatgccg caacatgacc aattacatcg ctatctgttt gaaaattttg ccgttcgcgg
    30361 cgaactggta accgtttcgg aaaccctgca acagatcctt gagaaccacg attatccgca
    30421 gcccgtcaaa aacgtgctgg cagaactgct ggttgcgacc agcctgttaa ccgctacgct
    30481 gaagtttgat ggtgatatca ccgtacagct gcagggcgac ggtccgatga atctggcggt
    30541 tattaacggt aacaataacc agcaaatgcg cggtgtggcg cgcgtgcagg gcgaaattcc
    30601 agaaaatgcc gacctgaaaa cgctggtcgg caatggttac gtggtgatca ccattacccc
    30661 gagcgaaggc gaacgctatc agggcgtagt tggtctggaa ggtgataccc tggcggcctg
    30721 cctggaagat tactttatgc gttctgaaca gctgccgacg cgcctgttta ttcgcaccgg
    30781 cgacgtagac ggcaaaccgg ctgcaggcgg tatgttgttg caggtaatgc ctgcgcaaaa
    30841 tgcccagcag gacgactttg accacctggc gacgctaacc gaaaccatca aaaccgaaga
    30901 actgctgacc ttaccggcaa acgaagtgtt gtggcgtttg tatcacgaag aagaggtgac
    30961 ggtttacgat ccgcaggatg tggagttcaa atgcacctgc tcgcgtgaac gttgcgccga
    31021 tgcgctgaaa acgctgcctg atgaagaagt tgatagcatc ctggcggaag atggcgaaat
    31081 tgacatgcat tgtgattact gcggtaacca ctatctgttc aatgcgatgg atattgctga
    31141 aatccgcaac aacgcgtctc cggcagatcc gcaagttcat taatgagtat gtccggcaga
    31201 gagggctctg ccggatttta cgactaatac acctgatgcg ctttgtcact cattcagatt
    31261 cacatccagc ctttcaccat ccactacaag atcgcgccac gctttaggct tcgttcccag
    31321 ttttccatcc ttcgcagctg tgagtaattt ttctttcgtt atatcacgcg gaagtaaact
    31381 catcgtaagc tcttgccatt ctttcttgtc aggatcaaag ccatagacaa tgtatctttc
    31441 atcatcaaag gcaaagagga tccgctctgc cctgccatca ctattcagat cttgctcgac
    31501 cagtacgcag gcgtcttttt caatacaggt catcacgtta tagcgttctt tgattaatgc
    31561 cgaccagaat gccgcatccg gtttgccaga acctggtgca attaacacat tttcggctaa
    31621 tactttttcc gatacctgtt gctgaagatg ttgctctcca tctaacgcca tcagcagatc
    31681 ccgcgcgcgt ttcgggtctt tcataaaccc ggcatcgctt ttcagcgact caagcgcagc
    31741 acgtccatag cgaccgctct gctcgagcat gtagatagtt acctggtctg acgtgttttt
    31801 gccgctctga taacgcgcca tatggctgtt cacgctaatg cgcatactgt ccagcaccgg
    31861 cgaattaaga agcaccagta tcaccaacac caataacgaa accgcaaggt tcactttgcc
    31921 ctgaagaaca acgggatttt gccctttacg ccagacgatg ctgacaaaat accccagcga
    31981 ccacaccagt aacaccagca ccgccagcac gccctgcaag cggtcgacag tccagccgta
    32041 ctgagcgacc cgcagccata acgcccaggc ggcgacgaac acatacagcg gagcaaccag
    32101 caaagcggtt ttaatcaggc aacgcaacgg ccctgtccag ggaagtgacg ctttttgcgg
    32161 atcgcggaca atagccatta agatcaattg caaaaaggcc agcgttaaca gcaacccggc
    32221 ggcggagatg tggcgagaaa tcgcgctcag gcccgtaaac ggcagagtga tgataaacat
    32281 cagggttagc aatgatacta acggcagcaa ccccgtggcg attaacgtga acaacttttg
    32341 aatagagtcg attaaacgtg actgtgttcg cgcgaggatc accgccagcg cggtaaccag
    32401 acctaacgtg agataaataa accagtcggt tgcaaaaaag agcgtattga aaaacgtgat
    32461 gccaacaagt ttaaacaact cactccacag taaaagcacc agccacgtca agccattggc
    32521 gagaaaaatc accagtaata taagtacgtt atgccatact gactggtaaa aatagcgata
    32581 gcggctactg tcattgcgga tgcgaaggct ttgttgtatc cacggtagca acaacattgc
    32641 catcaacagc agatagcagc caaaatccca aatcgccttt tcagctctcc aggggttcat
    32701 gccatcagtt tgccacttca accagccgct catccccagc gtggcaataa acaccagcgc
    32761 cagccatccc caaaggcgct tctgtttaaa agagatcacg gagaagagta aaacggatga
    32821 aaacgcaacg gtcgcaggca cgccataaac aatccaactg tgattttttc cggcaagcca
    32881 ggcgatcaac aggtagcaaa ccagcccctg aagtaatccg gtagcaatca tcccccagcg
    32941 tgttgccggt gaaagttcaa cgttatccat gttggttatc cagaatcaaa aggtgggtta
    33001 attatcgcat ccgggcagta gtattttgct tttttcagaa aataatcaaa aaaagttagc
    33061 gtggtgaatc gatactttac cgattgaatt tgcatcaatt tcattaagga atgcgattcc
    33121 actcacaata ttcccgccat ataaaccaag atttaacctt ttgagaacat tttccacacc
    33181 taaaatgcta tttctgcgat aatagcaacc gtttcgtgac aggaatcacg gagttttttg
    33241 tcaaatatga atttctccag atacgtaaat ctatgagcct tgtcgcggtt aacaccccca
    33301 aaaagacttt actattcagg caatacatat tggctaagga gcagtgaaat gcgcgttaac
    33361 aatggtttga ccccgcaaga actcgaggct tatggtatca gtgacgtaca tgatatcgtt
    33421 tacaacccaa gctacgacct gctgtatcag gaagagctcg atccgagcct gacaggttat
    33481 gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg ataccgggat cttcaccggt
    33541 cgttcaccaa aagataagta tatcgtccgt gacgatacca ctcgcgatac tttctggtgg
    33601 gcagacaaag gcaaaggtaa aaacgacaac aaacctctct ctccggaaac ctggcagcat
    33661 ctgaaaggcc tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt cgacgctttc
    33721 tgtggtgcga acccggatac tcgtctttcc gtccgtttca tcaccgaagt ggcctggcag
    33781 gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg aagagctggc aggtttcaaa
    33841 ccagacttta tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa agaacagggt
    33901 ctgaactccg aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct gattggcggc
    33961 acctggtacg gcggcgaaat gaagaaaggg atgttctcga tgatgaacta cctgctgccg
    34021 ctgaaaggta tcgcttctat gcactgctcc gccaacgttg gtgagaaagg cgatgttgcg
    34081 gtgttcttcg gcctttccgg caccggtaaa accacccttt ccaccgaccc gaaacgtcgc
    34141 ctgattggtg atgacgagca cggctgggac gatgacggcg tgtttaactt cgaaggcggc
    34201 tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta caacgctatc
    34261 cgtcgcgatg cgctgctgga aaacgtcacc gtgcgtgaag atggcactat cgactttgat
    34321 gatggttcaa aaaccgagaa cacccgcgtt tcttatccga tctatcacat cgataacatt
    34381 gttaagccgg tttccaaagc gggccacgcg actaaggtta tcttcctgac tgctgatgct
    34441 ttcggcgtgt tgccgccggt ttctcgcctg actgccgatc aaacccagta tcacttcctc
    34501 tctggcttca ccgccaaact ggccggtact gagcgtggca tcaccgaacc gacgccaacc
    34561 ttctccgctt gcttcggcgc ggcgttcctg tcgctgcacc cgactcagta cgcagaagtg
    34621 ctggtgaaac gtatgcaggc ggcgggcgcg caggcttatc tggttaacac tggctggaac
    34681 ggcactggca aacgtatctc gattaaagat acccgcgcca ttatcgacgc catcctcaac
    34741 ggttcgctgg ataacgcaga aactttcact ctgccgatgt ttaacctggc gatcccaacc
    34801 gaactgccgg gcgtagacac gaagattctc gatccgcgta acacctacgc ttccccggaa
    34861 cagtggcagg aaaaagccga aaccctggcg aaactgttta tcgacaactt tgataaatac
    34921 accgacaccc ctgcgggtgc cgcgctggta gcggctggtc cgaagctgta atgatttgaa
    34981 gctggagaat atctatccag tatcttatag aaagcaaaac gggaggcacc ttcgcctccc
    35041 gtttatttac ccttcttttg tcgtgccctg cgcccgcgtt accggcactg gcagccaggc
    35101 gcgaatggaa agcccgcccc gctcgctggt gccaagctcc agcatcccgt tatggttatc
    35161 cacgatacgc tgcacaattg ccagccctaa tcccgtgccg ctaatggtgc gcgcactgtc
    35221 gccgcggaca aacggctgga acaggtgctt acgttgttcc ggcgcaattc ccggaccgtc
    35281 atcttccacc tggaaccagg cgcgattcgg ctccgtcccg ctgctgactt tgatccagcc
    35341 attgccgtaa cgggcggcgt tgaccaccat attcgccacc gcgcgtttga tcgacagcgg
    35401 gtgcattttc acttcaatgc tgccggggta aagcgcggtt tcaatttccc gctcatagcc
    35461 actttcggca gcaatcacct caccgagtac tgcattaaga tccgccattt ccatcggcat
    35521 ctcctgcccg gtgcgcaggt agtcgataaa ctgctcaatg atggcgttgc actcttcgat
    35581 atctttattg atcgattctg ccagatagcc atcctgctcg ctcatcatct cggtcgccag
    35641 gcgaatacgc gtcagcggcg tgcgcaagtc gtgacttacc cccgccatca gcagcgtgcg
    35701 gtcatccgcc agttgcttaa caccagccgc catatggtta aaggcacggg taacggaacg
    35761 cacctcggac gcgccatact cacgcagcgg cggcggaata atccctttac caacctgcaa
    35821 ggctgcgtgt tcgagatcga ccaacggtcg gttctggata cgaataaaca gccacgcccc
    35881 gcctatcgcc aatagcataa tcgccagcgt atagcggaac agcggagaga aatcgccctg
    35941 atgaatttcg gtcagcggca cgcgtaccca gatattgggc gacagccagg ttttcagcca
    36001 gacgacaggc gaacttttgt tgacctcaac gcgcacttcc gtcgggccgc ccagttgctg
    36061 tgccatctga tggcttaaga attcatagtg ttgcgcccaa cgcagacctg cctcttcggc
    36121 agcctcgttg gagtagagag agatccccag ctcacggtag atctcccgac ggaaagcggg
    36181 aggcacaacc aactgcgtgc cgtcctccag ttgcagtttg tcggtcatca acatacgcac
    36241 ttcgtacgcg aggactttat taaactgctg gaggctcggc aaaatcgcga agttcagcac
    36301 caccagataa gtcgtcacca ggctggcgaa cagcaaggtg acgatgagca ataacgtacg
    36361 ggcaaatgaa cttcgtggcg agaagcgcaa tcgcctcatg ctttagagcc gtccggtaca
    36421 aagacgtagc ccaggcccca aacggtctga atgtaacgcg gatgcgctgg atcttcttct
    36481 accatgcggc gcagacgcga aatctgcacg tcgatggagc gttccattgc ggaatattca
    36541 cgaccacggg caaggttcat cagcttatcg cgggagagcg gctcacgcgg atggctgacc
    36601 agtgccttca gtaccgcaaa ctcaccgctg gtgagcggca tcggctcatc ttcgcggaac
    36661 atttcgcgcg taccgaggtt aagtttgaac ttaccgaaag caattaccgc ctcttcctgt
    36721 gacggtgcgc ctggcagttc gttcgcctga cgacgcagca ccgcacgaat acgggccagc
    36781 agttcacgcg ggttaaacgg ttttggaatg tagtcgtcag cgccaatctc caggcctacg
    36841 atacggtcca cttcttcccc tttcgccgtc accataatga tcggcatcgg gttgctctga
    36901 ctacgaagac gtcggcaaat cgacaagcca tcttcaccag gtaacattaa atccagtacc
    36961 ataagatgga aagattcacg agtcagcagg cgatccatct gttctgcatt agcgacgctt
    37021 cgaacctgga agccttgttc ggtgagataa cgttccagca gcgcacgcag gcgcatgtcg
    37081 tcatcgacca ccagaatctt gtagttctct tgcattgttt gtactcccaa aggttcgcaa
    37141 caatttgtaa gcgtgtattc ttaaaaaagc tcacgttcgt caccagctaa atctggtatg
    37201 aatttcagcc taaattgtta caaagcatat taaacagcag cttaagtata caatttattc
    37261 ggcgaaacat tattgattct gttgatatga tcacgttata cccaatgtgc gcattatcaa
    37321 acagacaaag ggaatcaacg agatgaaaac gcccctggtt acccgggaag ggtatgaaaa
    37381 actcaaacaa gagcttaatt atctctggcg tgaagaacgc ccggaggtca caaaaaaggt
    37441 gacctgggcc gcaagtctgg gcgaccgcag cgaaaatgct gactatcagt ataataaaaa
    37501 gcgtctgcgt gaaatcgacc gtcgcgtgcg ctatctcact aaatgcatgg aaaatctcaa
    37561 aatcgtcgat tactcccctc agcaggaagg caaagtcttt tttggcgcgt gggtggagat
    37621 tgaaaacgac gatggcgtga ctcaccgttt ccgtattgtc ggctacgatg aaatttttgg
    37681 ccgtaaagat tacatctcta tcgattcccc gatggcccgc gcattgctga aaaaagaagt
    37741 cggcgatctg gcggtggtga atacccctgc cggggaagcg agctggtatg ttaatgctat
    37801 cgagtacgtg aaaccgtaag gaagagtctt aacctcctgc cgatggctgg catttttgcc
    37861 agccagtccg tataactatc ccctgatttt tgatccgaaa agatgaactc aaaccatgat
    37921 gaatgattcg ttctgccgca ttattgcggg tgaaattcag gcgcgcccgg aacaggttga
    37981 cgctgccgtt cgcctgcttg acgaagggaa taccgtgccg tttatcgcac gttatcgtaa
    38041 ggaaatcacc ggcggtctgg atgacacgca gctgcgtaat ctggaaacgc gtctgagcta
    38101 tctgcgcgag ctggaagaga gacgtcaggc gatcctcaaa tccatttccg agcaaggcaa
    38161 actcaccgat gatctggcga aggccatcaa cgccacccta agcaaaaccg aactcgaaga
    38221 cctctacctg ccctacaaac ctaaacgccg cacccgcggg caaatcgcca ttgaagcagg
    38281 gcttgagccg ttggctgacc tgctgtggag cgatccgtca cacacgccag aagtcgccgc
    38341 tgcacaatat gttgatgccg ataaaggcgt ggcagatacc aaagccgcgc tggatggcgc
    38401 gcgctatatc ctgatggaac ggtttgccga agatgccgcg ctgctggcga aagtgcgtga
    38461 ttatctgtgg aagaacgcgc atctggtctc aaccgtggtg agcggtaaag aagaggaagg
    38521 ggcgaaattc cgcgactatt tcgatcatca cgaaccgttg tccacggtgc cttctcaccg
    38581 cgcgctggcg atgttccgtg ggcgtaacga aggcgtactc cagctttcgc tgaatgccga
    38641 tccgcagttc gatgagccgc ccaaagagag ctattgcgag caaatcatca tggatcacct
    38701 tggcctgcgc ctgaacaatg ccccggcgga tagctggcgc aaaggcgtag tgagctggac
    38761 ctggcgcatc aaggtgctga tgcatctgga aaccgaactg atgggtaccg tgcgcgaacg
    38821 cgcggaagat gaagcaatca acgtctttgc ccgtaacctg cacgatctgc tgatggcggc
    38881 ccctgccgga ctgcgtgcaa cgatgggcct cgatccgggt ctgcgtaccg gggtaaaagt
    38941 ggcggtggtc gatgccactg gcaaactggt ggcgaccgat accatttacc cgcacaccgg
    39001 acaagccgca aaagcagcga tgaccgttgc tgccttgtgt gaaaaacata acgttgaact
    39061 ggtggcgatc ggcaacggta cagcttcccg cgaaactgaa cgtttctatc tcgacgtgca
    39121 gaagcagttc ccgaaagtga ccgcacagaa agtgatcgtc agcgaagctg gcgcgtcggt
    39181 ttactcagct tccgagctgg cagcgcagga gttcccggat ctcgacgttt cgctgcgtgg
    39241 tgcggtatct atcgcccgcc gtttgcagga tccgctggcg gagctggtga aaatcgatcc
    39301 gaaatctatc ggcgtaggtc agtatcagca tgacgtcagc cagacgcaac tggcccgcaa
    39361 actggatgca gtagtagaag actgcgtaaa cgccgttggc gtcgatctca acaccgcttc
    39421 tgttccgcta ttaacccgcg tggcgggcct gacgcgcatg atggcgcaaa acatcgtggc
    39481 ctggcgcgat gagaacggcc agttcaagaa ccgtcagcaa ctgctgaaag tcagccgtct
    39541 ggggccgaaa gccttcgagc agtgcgcggg cttcctgcgc attaaccacg gtgataaccc
    39601 gctggacgcc tctaccgttc acccggaagc ctatccggtg gtggaacgca ttctggcagc
    39661 aacacagcag gcactgaaag atctgatggg taacagcagc gaactgcgta acctgaaagc
    39721 gtctgacttt actgatgaaa aattcggtgt gccgacagta actgacatca tcaaagagct
    39781 ggaaaaaccg ggtcgcgatc cgcgtccgga atttaaaacc gctcagtttg ccgatggcgt
    39841 cgagacaatg aacgacctgc aaccgggtat gatcctcgaa ggcgctgtca ccaacgtcac
    39901 caactttggc gcgtttgtcg atattggcgt gcatcaggac ggcctggttc acatctcttc
    39961 attgtcgaac aagtttgtgg aagatccgca taccgtggtg aaagcgggcg acattgtgaa
    40021 ggtgaaagtg ctggaagtgg atcttcagcg taaacgtatc gccctgacca tgcgtctgga
    40081 cgagcagcct ggcgaaacca acgcccgtcg cggcggcggt aatgaacgcc cgcaaaacaa
    40141 ccgcccggca gccaaaccac gcggtcgtga agcgcagcct gccggtaata gcgcgatgat
    40201 ggatgcgctg gcggcggcaa tgggcaaaaa acgttaaacg ccctgattga cccctaaacc
    40261 tgcgcgataa ggtgtccacg ccgcatctgg caacgaacgc cttgtctgac ataaaagtgc
    40321 cggagaatat ctccggcctt tttattccac aaccaaactc ataatatatt ccggcaatat
    40381 ttatcacttc attaacaact gaaaccttaa ttaaacatta gccagtccgg gtaattcact
    40441 attcgaatta tattttcgct gcgatataac cttgagccac atcaacattg agtcagatta
    40501 ttattcaaac caacattcgc acacatttta agtattgctg atagaaacca ttctcattat
    40561 cattgtgttg ttgattattt aatctctcct ttgttggcaa atcatctggt ctcatgtcgc
    40621 tgtcaaacgc cccatgaggt agttatccag ttaatgagaa acaagtaggc acctatgcaa
    40681 tacactccag atactgcgtg gaaaatcact ggcttttccc gtgaaatcag cccggcatat
    40741 cgccaaaaac tgctttctct tggcatgtta cctggctcct cttttaatgt ggtgcgcgtc
    40801 gctccactcg gcgaccccat tcatatcgaa acccgtcgtg tgagcctggt attacgcaaa
    40861 aaagatctgg ccttattaga agtggaagcg gtttcctgtt aatacggtga taacaacaat
    40921 gaaaaaatta accataggct taattggtaa tccaaattct ggcaagacaa ccttatttaa
    40981 ccagctcact ggcgcacgtc agcgtgtagg taactgggct ggcgttaccg tcgaacgtaa
    41041 agaagggcaa ttctccacca ccgatcatca ggtcacgctg gtggacctgc ccggcaccta
    41101 ttctctgacc accatctcat cgcagacctc gctcgatgag caaatcgcct gtcactacat
    41161 tttgagtggc gacgccgacc tgctgattaa cgtggtggat gcgtctaacc ttgagcgtaa
    41221 cctgtacctg acgctacaac tgctggaact cggcattccc tgcattgtgg cactgaacat
    41281 gctcgacatt gccgagaagc aaaatattcg tattgaaatt gatgctctgt cggcgcgtct
    41341 tggctgccca gtgatcccgc tggtttcaac ccgtggtcgc ggtattgaag cgctcaagct
    41401 ggcgattgat cgctataaag ctaacgagaa tgtggaactg gtgcattacg cacagccgct
    41461 actcaacgaa gcagattcac tggcaaaagt gatgccttcc gacatcccgc tgaaacaacg
    41521 tcgctggctg ggcctgcaaa tgctggaagg cgatatctac agccgcgcct acgccggtga
    41581 agcgtcgcag catctggatg ccgccctcgc ccgtctgcgt aatgagatgg acgatccggc
    41641 gctgcacatt gccgatgcgc gttaccagtg cattgctgcc atctgtgatg tggtaagcaa
    41701 caccctgacg gcagaaccca gccgtttcac cactgcggta gataaaatcg tgctcaaccg
    41761 tttcctcggt ctgccgattt tcctctttgt gatgtacctg atgttcctgc tggctatcaa
    41821 catcggcggg gcgttacagc cgctgtttga cgtcggctcc gtggcgctat ttgtgcatgg
    41881 tattcaatgg attggctaca cgctccactt cccggactgg ctgactatct tcctcgccca
    41941 gggcctgggt ggcggcatta acaccgtgct gccactggtg ccgcagattg gcatgatgta
    42001 cctgttcctc tccttccttg aggactccgg gtatatggcg cgtgcggcgt ttgtgatgga
    42061 ccgtctgatg caggcgctgg gcttgccggg gaaatccttt gtgccgttga tcgtcggttt
    42121 cggttgtaac gtaccgtcgg taatgggtgc acgtacgctt gatgcaccgc gtgaacgtct
    42181 gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg cgtctggcta tcttcgcagt
    42241 attcgcggct gccttcttcg ggcagaacgg tgcgctggcg gtcttctcgc tgtatatgct
    42301 gggtattgtg atggcggtgc tgactggcct gatgctcaag tacaccatca tgcgcggtga
    42361 agcgacgccg tttgtcatgg agttgccggt ctatcatgta ccacacgtta aaagcctgat
    42421 tatccagacc tggcagcgtc tgaaaggctt cgttctgcgt gctggtaaag tgatcatcat
    42481 cgtcagcatt ttcctgagcg ctttcaacag cttctcgctg agcgggaaaa tcgtcgataa
    42541 catcaacgac tcggcgctgg cttccgtcag ccgggtgatc accccggtct tcaagccgat
    42601 tggcgtacat gaagataact ggcaggcaac ggttggcctg tttacgggcg caatggcgaa
    42661 agaagtggtg gtgggtacgc tcaacaccct ctacaccgca gaaaatattc aggacgaaga
    42721 gttcaatccg gcggaattca acctcggtga agagctgttc agtgcggtag atgaaacctg
    42781 gcagagcctg aaagacacct tcagccttag cgtactgatg aaccccattg aagctagcaa
    42841 aggcgacggc gaaatgggta ccggggcgat gggcgtgatg gatcagaaat tcggtagcgc
    42901 agctgccgca tacagctacc tgattttcgt cctgctgtat gtaccgtgta tctcggtgat
    42961 gggggctatc gcccgtgaat caagccgtgg ctggatgggc ttctccatcc tgtgggggct
    43021 gaatatcgct tactcactgg caacattgtt ctatcaggtc gccagctaca gtcagcatcc
    43081 aacttacagc ctggtgtgca ttctggcggt tatcctgttt aacatcgtgg ttatcggtct
    43141 gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg gcaacccgca agtcggtaag
    43201 cagttgctgc gcagccagca ccaccggtga ttgccattaa tggcttcact tattcaggtg
    43261 cgcgatttgc tggcgttacg gggccgtatg aaagcggccc agataagcca gacattgaac
    43321 actccacagc caatgattaa cgccatgctg caacaactgg aaagtatggg caaagccgtg
    43381 cggattcagg aagaacctga cggctgcctc tctggcagtt gtaaaagctg cccggaagga
    43441 aaagcctgtc tgcgcgagtg gtgggcgctg cgttaacctt actccatcgc ctgttttgga
    43501 aagcccggta tgcgtctgca tccgggcttt tttgcgtgcg gctttccata aaaatgcaac
    43561 tcttgcagca cggcgtaagt tcctttgaaa gcatctcgca gggatgaaaa ctcgctaata
    43621 cacaggagtg aagtggcgcg tagagtcgcg gcattcaaac aacaggtgaa ggaacgccat
    43681 gagcaaaaag cagagttcca ccccacacga tgcgctgttc aaactctttt tacgccaacc
    43741 ggacacggct cgtgattttc ttgcgtttca tttaccggca cccattcacg cgctttgtga
    43801 tatgaaaacc ctcaagctgg agtcgagcag ctttattgat gacgatctgc gtgaaagcta
    43861 ttccgatgtg ctgtggtcgg tgaaaacgga acaaggacca ggatacatct attgtctgat
    43921 tgaacatcaa agcacctcaa acaaactgat cgcatttcgc atgatgcgtt acgctattgc
    43981 cgcaatgcaa aatcaccttg atgccggata caaaacgttg ccgatggtgg tgccattgtt
    44041 gttttaccac ggtattgaaa gcccctatcc ctattcgctg tgttggctgg attgtttcgc
    44101 cgatcccaaa ctggcaaggc agctttatgc ctccgcattt ccgctgattg atgtcaccgt
    44161 catgcctgat gatgaaatca tgcagcaccg acgtatggcg ctgctggagt taattcaaaa
    44221 acatattcgt caacgcgacc tgatggggca ggtagagcaa atggcctgct tattaagtag
    44281 tggatacgct aatgacagac aaatcaaagg gctgtttaat tacatactgc aaaccggcga
    44341 cgcggtacgt tttaacgatt ttatcgacgg cgttgccgaa cgttcaccga aacacaagga
    44401 gagtttaatg acaattgcgg aaagattgcg gcaggagggg gaacaatcca aagccctgca
    44461 tatagccaaa ataatgcttg aatccggagt ccctcttaca gacatcatgc gctttaccgg
    44521 ggtgtcagaa gaagagttgg ctgcggcgag ccagtaaatt tctgtctcgc catttcaaaa
    44581 gttgcctaca ccctctgctt caacgccacc agcaggtgac aaaactcgac cggatgcgaa
    44641 ataaacggcg catgggccgc tttggcgaag atatatgatt cgctgtgagg ccaaagttta
    44701 tccagcatcg gcaccacttt gcgcggcacc agaccgtcga gatagccata caatcgcaaa
    44761 aacggcatgg gcacgttttg cagcggctga cggagatcga ccgttttcag gatttccagc
    44821 ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt cttcaacgcc
    44881 cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta acgccaggaa ccgctccact
    44941 gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagcacgtc cggttttatc
    45001 cccggccact cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac cagcgcctga
    45061 acacgctcgg gatgggttaa cgcaatctgg cttgccacca gcccgcccag actccagcct
    45121 aaccaaatgg ctttatcagg tgcctgttgc agcacggctt cggccatatc agcaagtgac
    45181 agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa
    45241 tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc
    45301 agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc
    45361 tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg attatgctgg
    45421 ctatgccgaa tgccactggc gttaggtcat tgggggattt gttcggtctg ctcacgcgct
    45481 gcccgtacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca ctcacatctt
    45541 ccctgcggtc gctgcctgca aaaaccaccg ccctggcaaa gactggtcac ggttgccgac
    45601 tatgcgccgc cgttaagtcc gcttatccac cagcttaaat tttcccggcg cagtgaaatc
    45661 gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg caccaccggg
    45721 ttgcaattgc cggatcgcat catcagcgtt ccgttatggc agcggcgtca ctggcgtcgg
    45781 ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca ctgccgatgg
    45841 gatagcaaag ccgtcacacg aacacgggcc actgcgaccc agcattttct cagtgcccgg
    45901 ctacgcaagc gcaacctgaa aaatgccttt cgtcttgaat tgcccgtgca aggtcgccat
    45961 atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg
    46021 cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc
    46081 ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat
    46141 ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc aggaagaagg
    46201 gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggtgtttc
    46261 ttattgtccg ccggatgccg tggaagccac cgacactgcc ctgaaatttg acctgctgac
    46321 cgcgtatgtt gatgagttaa gcgcaccata cctggaagat gcagagatcg actttgttac
    46381 cgaccagttg ggttctcagt taacgctgaa agccccgaac gccaaaatgc gtaaagtggc
    46441 agacgatgca ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga tcaacccaca
    46501 gcttgctggt cacggtggcc gcgtttcact gatggaaatc accgaagacg gttacgccat
    46561 tctgcaattt ggcggcggct gtaacggttg ttccatggtc gatgtgacgc tgaaagaagg
    46621 gatcgagaag cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg atctcaccga
    46681 acaccagcgc ggcgaacact cctactacta agttatcccc tcttttgtag attgcccgat
    46741 gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt ggcgcagcat
    46801 tgctttggca aatcccttcc ttcgtttctc cgcgataata tgaccaacct ctcataattt
    46861 aaatttaccc cgctctggtg attctcaaac gccagatgtt acccgtatca ttcacatggg
    46921 taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta tcagtcgtgg
    46981 tgccgcaata tctctgttcc cgattgggat aattagagtt tgtcgtcaga aaattgacgt
    47041 tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg ctatcggtgt
    47101 aatcttgttg ttgctcctga tgatccgctt caaaatgaac ggcttcatcg ctctcgtcct
    47161 cgtggcgctt gctgttggat taatgcaagg aatgccgctg gataaagtta ttggctccat
    47221 caaagccggt gtcggcggga cgctcggtag ccttgccctg atcatgggtt ttggcgcaat
    47281 gctgggcaaa atgctggcag actgcggtgg cgcacaacgt atcgccacca cgctgattgc
    47341 caaatttggt aaaaagcaca tccagtgggc ggtggtgtta accggtttta ccgttggttt
    47401 tgccctgttc tatgaagtgg gcttcgtgct gatgctgcct ctggtgttta ccatcgcggc
    47461 ttccgcgaat attccactgc tttatgttgg tgtaccaatg gcggctgcac tgtctgtgac
    47521 ccacggcttc ctgccaccgc atccgggccc gactgcgatt gccaccattt tcaatgccga
    47581 tatgggtaaa accctgctgt acggtactat tctggcaatc ccgaccgtga ttctcgccgg
    47641 tccggtttac gctcgcgtgt tgaaaggtat cgataagcca atcccggaag gtctctacag
    47701 cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc gtcagcgtct ggacttctct
    47761 ggtgccggtt gtgctgatgg cgatgcgtgc gattgccgaa atgatcctgc cgaaaggtca
    47821 cgctttcctg ccggtagcgg agttcctcgg tgacccggta atggctacgc tgattgccgt
    47881 gctgattgcc atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc agattaacga
    47941 cacgctggtt tcttccatca aaatcattgc gatgatgctg ttgatcatcg gtggtggcgg
    48001 tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa tacattgctt ccatgatgca
    48061 cgaaaccaac atttctccgc tgctgatggc ctggtcgatt gctgccgtac tgcgtatcgc
    48121 gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac cgctgattgc
    48181 aacgacgggt gttagcccgg agctgatggt tattgcggtg ggttccggta gtgtgatttt
    48241 ctctcatgtg aacgatccgg gcttctggct gttcaaagag tactttaacc tgactatcgg
    48301 cgagaccatc aaatcctggt cgatgctgga aaccattatc tcggtgtgcg gtctggtagg
    48361 ctgtctgctg ctgaatatgg tgatttgata acactgactg ccggatgcat cgcatccggc
    48421 atcaaccgca ctctacttct tcttcgctgc agctctgcgc cgtctgtcca aatccttcag
    48481 caacttgttc acgccatcat cggcaaacat cgactcaagc gttgcggaaa gcttgcgtcg
    48541 ccagttttta tactggtaac tggtgccagg aatattcacc ggttcggcca tatccagcca
    48601 gtcttccggc tgtagtccta acagagcact gttactgtcg gcaatgtagc gctgcaaacc
    48661 acggttcagc gtcggcgtca tcgacatcaa cgatgcctta tgcccggcac gttttggcag
    48721 acaaccatat ttatgcagtg catccagcag cccttgcttc gccagttcgc gatcctgata
    48781 cagaccgcgc agtaccactt catccggata cagccccagg gttttgccca gcgttagatc
    48841 cccgctctcc cagtaaccgc gtagcgtagg caggtcatgt gtcgccgcaa ccgccatcga
    48901 ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg tggtcgtttt cgaaatagag
    48961 cactttgtag gagtacacac cgctgctgcg cagcttaccg acaatctcta ccggtacggt
    49021 accgagatct tcaccaatca ccatacagcg atgacgttta ctttcgagtg ccagaatcga
    49081 gagcagatca tccaccggat agtgaacata cgcgccctga tctgccgtct cgccatacgg
    49141 tatccaccac aaacgcagca tcgacatcac atggtcaatt cgtaatgcgc cgcagttttg
    49201 catattggca cgcaacagct cgataaacgg ttcataggca cgcgcggtga tgatatgcgg
    49261 gtccattggc ggtaatcccc agttctgccc caacgggccg aggatatccg gcggcgcgcc
    49321 aaccgacgct ttcaggcaat atagttcacg gtcacaccag gtttccgccc caccttccgc
    49381 tacgccaacc gccagatcac gatacaagcc aatcggcatt tcatagccct ggcttatctc
    49441 ccagcaggcg gcaaactggc tgtaagccag ccactgcaac cagagataaa aatcgacgtc
    49501 atcacgatgt tcttcgcaga actgacgcac ttctggtgaa tccacgttct gatacatctc
    49561 tggccatgca ggccagcccc agcgcatttc gtcctctttc acttgctggg catgtagcgc
    49621 atcaaaggct gcctgccaga acaggctgtc gccctgctct gcaacaaact ggcgaaacgc
    49681 ggtcatctgc tcatcatcac gttgcgcgaa acctttccac gccattcgta atgctgtcat
    49741 ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca tcgcgcgcct gttgcagcgt
    49801 ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct tcgctaagat ggaaatcttc
    49861 aacggcgtta acgtcgatat aaatcacatt cagccaacgg cgagaagacg ggctgtatgg
    49921 gctggcactc tccggatttg ccggatagag cgcatgaatc gggttcaggc cgatgaacga
    49981 cccgccacgt tttgccacat ccaccagcat cgctttgaga tcgccaaaat ccccaatacc
    50041 ccagtttttt tccgatcgca gcgtataaag ctgaacgcag gcaccccaca gcttttgttt
    50101 attcagcaac gcctgcggtt cgtaacagcg tttcggggcg acaatcaccc ggcaatgcgc
    50161 gcgctggtcg tcctgggtga gtgtcagcgt gtgataacct tccggcagct tcgtcggtag
    50221 attgaacgct ttgccccccg ttacatggcc tttgtactgt gttccttctt cggtggtcag
    50281 cagccagcta tattcgccgc tgccctccac caccatcggc atttttttgc cgctggtata
    50341 aaccatgaca ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt gatgcatcgc
    50401 gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt gggcattgat
    50461 gtaattgggg ctaattcccg ccgccagcgc ggcattatcc agacgtttgc tttccatcga
    50521 gcttccttag cgttttgcct gccagatacg agcctgataa tcgcgaatag agcgatccga
    50581 gctaaacata ccgcagcggg cggtattgag gatcgccgcg cgagtccagg cctcctggtc
    50641 gcggtacagc acatccacct gcttttgtgc ctcaacatag gctgcgaaat ccgccatcac
    50701 cagatacgga tcgccgcctt gtttgccgat actgtgcagc atctggtcga aggcatgctt
    50761 atcgccgtcg ctgtatttac cgctttccag ctctttcaat actgcgtcca gcaccttatc
    50821 tttcttccgc catttcaccg ggtcgtagcc tttggccaga atagccttca cttgttccac
    50881 ggtatgacca aaaataaaga tattttcttc accgactttc tcggcgattt caacgttcgc
    50941 accatccagc gtaccgacag taagcgcacc attgagcgcc agtttcatat tgccggtacc
    51001 ggaagcttct ttacctgcag tcgaaatttg ttcggagata tccgccgccg ggatcagttt
    51061 ttccgccgcc gaaacgcaat aatccggcag gaacaccacc ttcaacttat cgccaaccag
    51121 tggatcgttg ttgatcacgt cagccacttt gttgatcgca aagataatat tcttcgccag
    51181 gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg cgcggtacgc gatcagcctg
    51241 cgggttttca cgaatttctt tgtacaacgc cagaatatgc agcagattca ggtgctggcg
    51301 tttgtactca tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat tgatctcaat
    51361 accggtacga actttcacaa actccgccag acggacttta ttcgcctgct tgatctcgcg
    51421 atattgctga cggaatttcg catcatcagc gaatttttcc agattgatca gctgatcgag
    51481 atcgttagcc cactcttttt gcagtgattt atccaacaga gccgccagtg cagggttgca
    51541 ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg ttatggaatt tgttcggcca
    51601 tagctggtga tattccggga acagatcttt caccaccaga tccgagtgca gcgccgcaac
    51661 accgttcacc gcgaaaccgc caaccacaca caggttcgcc atatgcactt gtttatcgtg
    51721 caccaccgcc agtttggccc acactttttc atcgcccggc caggttttct ccaccagcgt
    51781 tttaaagcga gtattaattt cgttaataat ctgcatatgg cgcggcagta agcctttcac
    51841 cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg gtatggttgg tgtaagcgaa
    51901 agttttgctg gtaatggccc aggcgtcatc ccagctcatc tggtgctcat cgatcagcac
    51961 gcgcagcagt tctggaatcg cgatagttgg gtgggtatcg ttcagctgaa taacttcgta
    52021 atccgccagt tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa tatccgctac
    52081 cgaacaggca cactggaagt actgctgcat caagcgcagc tttttaccgg cagtatggtt
    52141 gtcgtttgga tagagaactt tggtcagttt ttccgcattg atgccctgct gttcggcacg
    52201 caggaaatca ccgtcgttaa atttagtcag atcaaacgga tgcgcgtgcg tcgcctgcca
    52261 cagacgcagc ggctgcgcca cgccattacg atagccgaca acggggagat cccacgcttg
    52321 accggtaatg gtaaactccg gctcccagcg tccgtctttc gtcactttac cgccaatccc
    52381 tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttactgc ggtgccagtc
    52441 atccggcgct tcaacctgtt tgccatcgac aaaagactgg cggaacaaac catattgata
    52501 gttcagaccg taacccgtcg cagactgacc gacagttgcc attgagtcga ggaagcacgc
    52561 cgccagacgt cccagaccac cgttacccag cgccgggtcg atctcttctt ccagcaggtc
    52621 cgtcagattg atgtcataag ccttcaacga atcctgtaca tcctgatacc agccgagatt
    52681 caacaggttg ttgcccgtca ggcgaccaat caaaaactcc attgagatgt agttaacatg
    52741 tcgctgattc gccaccggct tggcgaatgg ctgagcacgc agcatttcgg ccagtgcttc
    52801 actcactgct agccaccact ggcgaggagt catttcagcc gcagaattta agccataacg
    52861 ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag gttgtgacat
    52921 aggagttcca cttttcttag attttcaaca caacgttatc gctagtttgc caggctcgat
    52981 gttgaccttc ctcatcctgc gggggattag gcagggagga gttgcgggga tgagcaagga
    53041 aatgtgatct caaccactta aagctagtgc aaaccacagg attagcagca aatcaatgca
    53101 atacagcgca gaaaatctgt atctaagtgc aaaaaatggc cgttgcgtat tttcaaaaag
    53161 cggaaggtaa ctctataaat taagtaaagg agtgaaacag tttcataagt aaaatatcca
    53221 gtgtg
//
LOCUS       DAZFRN010000014        52850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFRN010000014 DAZFRN010000000
VERSION     DAZFRN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.014"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..828
                     /gene="ynaI"
                     /locus_tag="ACHKUB_000799"
     CDS             <1..828
                     /gene="ynaI"
                     /locus_tag="ACHKUB_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO6653856.1"
                     /translation="KLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDI
                     TSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSG
                     IMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVEN
                     PGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSL
                     NIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
     gene            complement(879..2492)
                     /gene="mppA"
                     /locus_tag="ACHKUB_000800"
     CDS             complement(879..2492)
                     /gene="mppA"
                     /locus_tag="ACHKUB_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO6653857.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2829..3728)
                     /gene="pgrR"
                     /locus_tag="ACHKUB_000801"
     CDS             complement(2829..3728)
                     /gene="pgrR"
                     /locus_tag="ACHKUB_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO6653858.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3866..4786
                     /locus_tag="ACHKUB_000802"
     CDS             3866..4786
                     /locus_tag="ACHKUB_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6653859.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            4786..5718
                     /locus_tag="ACHKUB_000803"
     CDS             4786..5718
                     /locus_tag="ACHKUB_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6653860.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     KQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKSHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            5736..6374
                     /locus_tag="ACHKUB_000804"
     CDS             5736..6374
                     /locus_tag="ACHKUB_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6653861.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            6665..7393
                     /gene="mpaA"
                     /locus_tag="ACHKUB_000805"
     CDS             6665..7393
                     /gene="mpaA"
                     /locus_tag="ACHKUB_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO6653862.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLSQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(7368..8333)
                     /gene="ycjG"
                     /locus_tag="ACHKUB_000806"
     CDS             complement(7368..8333)
                     /gene="ycjG"
                     /locus_tag="ACHKUB_000806"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO6653863.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            8452..8958
                     /gene="tpx"
                     /locus_tag="ACHKUB_000807"
     CDS             8452..8958
                     /gene="tpx"
                     /locus_tag="ACHKUB_000807"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO6653864.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(9002..10543)
                     /gene="tyrR"
                     /locus_tag="ACHKUB_000808"
     CDS             complement(9002..10543)
                     /gene="tyrR"
                     /locus_tag="ACHKUB_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO6653865.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(10691..11752)
                     /gene="ycjF"
                     /locus_tag="ACHKUB_000809"
     CDS             complement(10691..11752)
                     /gene="ycjF"
                     /locus_tag="ACHKUB_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO6653866.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(11749..13146)
                     /gene="ycjX"
                     /locus_tag="ACHKUB_000810"
     CDS             complement(11749..13146)
                     /gene="ycjX"
                     /locus_tag="ACHKUB_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO6653867.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13301..14299
                     /gene="ycjW"
                     /locus_tag="ACHKUB_000811"
     CDS             13301..14299
                     /gene="ycjW"
                     /locus_tag="ACHKUB_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6653868.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(14410..15315)
                     /gene="ompG"
                     /locus_tag="ACHKUB_000812"
     CDS             complement(14410..15315)
                     /gene="ompG"
                     /locus_tag="ACHKUB_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO6653869.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(15360..16442)
                     /locus_tag="ACHKUB_000813"
     CDS             complement(15360..16442)
                     /locus_tag="ACHKUB_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6653870.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(16456..17115)
                     /gene="pgmB"
                     /locus_tag="ACHKUB_000814"
     CDS             complement(16456..17115)
                     /gene="pgmB"
                     /locus_tag="ACHKUB_000814"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO6653871.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFLAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(17112..19379)
                     /gene="ycjT"
                     /locus_tag="ACHKUB_000815"
     CDS             complement(17112..19379)
                     /gene="ycjT"
                     /locus_tag="ACHKUB_000815"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO6653872.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     LFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLEYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            complement(19376..20431)
                     /gene="ycjS"
                     /locus_tag="ACHKUB_000816"
     CDS             complement(19376..20431)
                     /gene="ycjS"
                     /locus_tag="ACHKUB_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6653873.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(20441..21229)
                     /gene="ycjR"
                     /locus_tag="ACHKUB_000817"
     CDS             complement(20441..21229)
                     /gene="ycjR"
                     /locus_tag="ACHKUB_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO6653874.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIGVPAAW
                     GMFTFRLPPMTSPRSLNGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(21247..22299)
                     /gene="ycjQ"
                     /locus_tag="ACHKUB_000818"
     CDS             complement(21247..22299)
                     /gene="ycjQ"
                     /locus_tag="ACHKUB_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO6653875.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(22330..23172)
                     /gene="ycjP"
                     /locus_tag="ACHKUB_000819"
     CDS             complement(22330..23172)
                     /gene="ycjP"
                     /locus_tag="ACHKUB_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6653876.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(23159..24040)
                     /gene="ycjO"
                     /locus_tag="ACHKUB_000820"
     CDS             complement(23159..24040)
                     /gene="ycjO"
                     /locus_tag="ACHKUB_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6653877.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(24061..25353)
                     /gene="ycjN"
                     /locus_tag="ACHKUB_000821"
     CDS             complement(24061..25353)
                     /gene="ycjN"
                     /locus_tag="ACHKUB_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6653878.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(25367..27046)
                     /gene="ycjM"
                     /locus_tag="ACHKUB_000822"
     CDS             complement(25367..27046)
                     /gene="ycjM"
                     /locus_tag="ACHKUB_000822"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO6653879.1"
                     /translation="MKQKITDHLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(27259..27573)
                     /gene="pspE"
                     /locus_tag="ACHKUB_000823"
     CDS             complement(27259..27573)
                     /gene="pspE"
                     /locus_tag="ACHKUB_000823"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO6653880.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(27648..27869)
                     /gene="pspD"
                     /locus_tag="ACHKUB_000824"
     CDS             complement(27648..27869)
                     /gene="pspD"
                     /locus_tag="ACHKUB_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO6653881.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(27878..28237)
                     /gene="pspC"
                     /locus_tag="ACHKUB_000825"
     CDS             complement(27878..28237)
                     /gene="pspC"
                     /locus_tag="ACHKUB_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO6653882.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(28237..28461)
                     /gene="pspB"
                     /locus_tag="ACHKUB_000826"
     CDS             complement(28237..28461)
                     /gene="pspB"
                     /locus_tag="ACHKUB_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO6653883.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(28515..29183)
                     /gene="pspA"
                     /locus_tag="ACHKUB_000827"
     CDS             complement(28515..29183)
                     /gene="pspA"
                     /locus_tag="ACHKUB_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO6653884.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            29350..30327
                     /gene="pspF"
                     /locus_tag="ACHKUB_000828"
     CDS             29350..30327
                     /gene="pspF"
                     /locus_tag="ACHKUB_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO6653885.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(30447..31712)
                     /gene="puuE"
                     /locus_tag="ACHKUB_000829"
     CDS             complement(30447..31712)
                     /gene="puuE"
                     /locus_tag="ACHKUB_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO6653886.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYVDKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(31750..33030)
                     /gene="puuB"
                     /locus_tag="ACHKUB_000830"
     CDS             complement(31750..33030)
                     /gene="puuB"
                     /locus_tag="ACHKUB_000830"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO6653887.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(33032..34519)
                     /gene="puuC"
                     /locus_tag="ACHKUB_000831"
     CDS             complement(33032..34519)
                     /gene="puuC"
                     /locus_tag="ACHKUB_000831"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO6653888.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(34794..35351)
                     /gene="puuR"
                     /locus_tag="ACHKUB_000832"
     CDS             complement(34794..35351)
                     /gene="puuR"
                     /locus_tag="ACHKUB_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO6653889.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(35378..36142)
                     /gene="puuD"
                     /locus_tag="ACHKUB_000833"
     CDS             complement(35378..36142)
                     /gene="puuD"
                     /locus_tag="ACHKUB_000833"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO6653890.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            36354..37772
                     /gene="puuA"
                     /locus_tag="ACHKUB_000834"
     CDS             36354..37772
                     /gene="puuA"
                     /locus_tag="ACHKUB_000834"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO6653891.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            37921..38085
                     /locus_tag="ACHKUB_000835"
     CDS             37921..38085
                     /locus_tag="ACHKUB_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653892.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            38075..39460
                     /gene="puuP"
                     /locus_tag="ACHKUB_000836"
     CDS             38075..39460
                     /gene="puuP"
                     /locus_tag="ACHKUB_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO6653893.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            39594..39839
                     /gene="ymjA"
                     /locus_tag="ACHKUB_000837"
     CDS             39594..39839
                     /gene="ymjA"
                     /locus_tag="ACHKUB_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO6653894.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            40151..41794
                     /gene="sapA"
                     /locus_tag="ACHKUB_000838"
     CDS             40151..41794
                     /gene="sapA"
                     /locus_tag="ACHKUB_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO6653895.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            41791..42756
                     /gene="sapB"
                     /locus_tag="ACHKUB_000839"
     CDS             41791..42756
                     /gene="sapB"
                     /locus_tag="ACHKUB_000839"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO6653896.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            42743..43633
                     /gene="sapC"
                     /locus_tag="ACHKUB_000840"
     CDS             42743..43633
                     /gene="sapC"
                     /locus_tag="ACHKUB_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO6653897.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            43633..44625
                     /gene="sapD"
                     /locus_tag="ACHKUB_000841"
     CDS             43633..44625
                     /gene="sapD"
                     /locus_tag="ACHKUB_000841"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO6653898.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            44627..45433
                     /gene="sapF"
                     /locus_tag="ACHKUB_000842"
     CDS             44627..45433
                     /gene="sapF"
                     /locus_tag="ACHKUB_000842"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO6653899.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            45501..45854
                     /gene="ycjD"
                     /locus_tag="ACHKUB_000843"
     CDS             45501..45854
                     /gene="ycjD"
                     /locus_tag="ACHKUB_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO6653900.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            46224..47012
                     /gene="fabI"
                     /locus_tag="ACHKUB_000844"
     CDS             46224..47012
                     /gene="fabI"
                     /locus_tag="ACHKUB_000844"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO6653901.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVIHVDGGFSIAAMNELELK"
     gene            47157..48284
                     /gene="yciW"
                     /locus_tag="ACHKUB_000845"
     CDS             47157..48284
                     /gene="yciW"
                     /locus_tag="ACHKUB_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO6653902.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            48352..50286
                     /gene="rnb"
                     /locus_tag="ACHKUB_000846"
     CDS             48352..50286
                     /gene="rnb"
                     /locus_tag="ACHKUB_000846"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO6653903.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(50364..50468)
                     /locus_tag="ACHKUB_000847"
     CDS             complement(50364..50468)
                     /locus_tag="ACHKUB_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653904.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            50522..52507
                     /gene="pdeR"
                     /locus_tag="ACHKUB_000848"
     CDS             50522..52507
                     /gene="pdeR"
                     /locus_tag="ACHKUB_000848"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6653905.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(52489..52617)
                     /gene="ymiD"
                     /locus_tag="ACHKUB_000849"
     CDS             complement(52489..52617)
                     /gene="ymiD"
                     /locus_tag="ACHKUB_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO6653906.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            52654..52827
                     /gene="yciZ"
                     /locus_tag="ACHKUB_000850"
     CDS             52654..52827
                     /gene="yciZ"
                     /locus_tag="ACHKUB_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO6653907.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT        12636 a        13908 c        13164 g        13142 t
ORIGIN      
        1 aaactcaaat ttatcacccc aggcgttatc gattttatct gtacgtccct gattgcggtt
       61 attctgacga ttaagctatt tttgctgatt aatcagtttg aaaaacagca gattaaaaaa
      121 ggtcgcgata tcaccagtgc gcggattatg tcgcgcatta tcaaaatcac cattattgtg
      181 gtgcttgttc tgctttatgg cgaacatttc ggcatgagcc tttctggctt gctgaccttt
      241 ggtggtattg gtggtctggc tgtcggtatg gccggtaaag atattctgag taatttcttt
      301 tccgggatca tgctctattt cgaccgtcct ttcagtattg gcgactggat ccgttcaccg
      361 gacagaaata tcgaaggtac agtagcggaa attggctggc gaattaccaa aattacgacc
      421 tttgataatc gtccattgta cgtaccgaac tcgctgtttt catcgatcag cgtagaaaac
      481 ccaggacgaa tgaccaaccg ccgcattacc acgaccattg gtttacgtta tgaggatgcg
      541 gcaaaagtgg gcgttattgt cgaagccgta cgtgagatgc tgaaaaatca cccggccatc
      601 gaccagcgac aaaccttact ggtttatttc aaccagtttg ccgactcgtc attgaatatt
      661 atggtttatt gctttaccaa aaccacggta tgggctgagt ggcttgctgc acagcaagac
      721 gtttatttga agattatcga tattgtacag tcacacggcg cggactttgc cttccccagc
      781 cagacgctgt atatggataa cattacaccg ccggaacagg gtcgctaaat cagacatcac
      841 tggcgttatc cacaagggta acgccagttc ccatcacatc aatgcttcac aatatacata
      901 gtccggctgt acgccacatc ttccgggtta ttaatgggat aacctttcag ccacggcttg
      961 attaatcgtc cattggtata ttgataaatt ggtgcaatcg gtgcttgctc catgaggatt
     1021 ttttctgccg cgttgtaatc ggcattacgc gctttaacgg tattttccgt cgatgcctgg
     1081 gccagaactt tgtcgtatgc cggattgtta aagcgtgaaa tatttcctga atgcgttgac
     1141 gttaataatg tcaggaaagt ggagggttca ttataatccc ccacccatga ggcacggata
     1201 acatcaaaat tgccggtgtt acggctatcg atataggttt tccattcctg attttgcaat
     1261 ttaacatcta cgccaaggtt ctttttccac atcgatgcta cagcaatcgc aattttttga
     1321 tggttttctg aagtgttata caaaagcgtc agcttcagcg gtttttgcgg accataacca
     1381 gctgcgctca acaaagtttt tgcctgcgca ttcagttctt cctgactcat ttgttcaaac
     1441 ggcgaaggtt ccggcgtaaa tccggcggta acatctggtg taaaatgcca cgctggcttt
     1501 tcgcccgttc ccaacacttt ttcggtcatc aggcggcgat ctatcgtcat acttaatgcc
     1561 aggcgaacgc gctgatctgc cgtcggccct ttttgcgtgt taaacgcata ataataggtc
     1621 ccgagctgtg gcggcgtata aacctgcccc ggaatatcct tcaacagctt ctgatacata
     1681 tttttcggga aggattcggt gatatcaata tcccccgcga ggtaacgctt agtggctgcg
     1741 gattcctgat taattggcag gaaggtcact ttttgcagta ccgttttggc gttatcccaa
     1801 taatgggtat tcggtaccac gaccagtttt tcattgacta cgcgatcttt aagaacataa
     1861 gcgccattgc cgatcagatt tccgggtttc gtccactctt taccgctttc tacgttggct
     1921 ttttgcaccg ggaagaaggc aaagttagcg gttaaattca caaaccacgg caacggttta
     1981 tcaagctgaa ttttcaaggt atgggcatca actgcggtga cgccaagctg gtcaggcgta
     2041 gctttaccat caataatcgc ctgtgcgttg ttgattcccg ccagcgcggc aaaccatgca
     2101 aatggcgata gcgttttcgg gtccaccaga cgctgccagc tgtagacaaa atcttgcgcc
     2161 gttaccggtg tgccatccgc ccattttgcg ttatcgcgca gggtaaaagt ccagatacgg
     2221 ttgtcattac ttttccactg agtcgcaacg ccggggacaa tctccccttt ctcgttctga
     2281 ttcaccagtc cttcaaacag atcgcgaatg acctgaatct ctggcaggcc cacggcttta
     2341 gcgggatcca gcgacgcagg ctcatcttta atgtggcgca ccagctcctg cttctctgcc
     2401 agtactgtgc cgctcggaac ttctgcagca tacgaaagag aaatgctgct gaccaacagc
     2461 gcacaacacg tgactgaaac agagtgcttc ataagtttcc ctctaaccga cattaaaatt
     2521 gatgcgtaat tatttgtttc gccttaaaga aatgcaaaca acttccatca aaaaagtgac
     2581 acaggaagca taaaatgttt tatgtatcaa tatattaaat cagatttagt gtaaataatc
     2641 gacgccaaaa tgcggcaaag cacatagaac accgaacact acgttcaggc ttcgcacgtt
     2701 caaaccagtt ttcggtatca aaatcagcat ctgtcgccat tgtacgaaga ataaaaaata
     2761 accaaaacgt tgaaaggaac tgagaatacg ttaagagaga aattaaatta atgtatcagc
     2821 aagtagtgct aaacagcacc tttatacttc agcctatcta taaataatga gaatgccgag
     2881 ccagcatgtc gacggtgtgg atagtacagg tgataaccgg gtaaatctgg tgtgaattta
     2941 tccaaaacac gtatcagttt tttttcttta atagcccgct caacctgatc ataaggtaga
     3001 tacgccaatc catgcccatc aattgcagca tcaatgatca ggtctatcgt attcagtaaa
     3061 agctgacctt ccatgcgaac acgaacttca cgtccaccgc gtattaatct ccagcgattt
     3121 gctgtacccg atgtgggaag atacaaatta attgcctgat gatctattaa ttgtgacact
     3181 gacgttggaa cacttcggcg agaaaaataa tccggtgagc caacaatagc cattggtata
     3241 tctggcccga ttcgaatggc gatcatatct ttatccatct ccccacccag acggacgcct
     3301 gcatcaaaac gttcagaaac gacatcggtc aaaccataat caatggtgag ctgaatatca
     3361 atttcaggat gcgatttcag gaatgtgcgc attgctggta acaatatcgt ttttgctgca
     3421 tgttctacag tagtaatacg tattgtcccg gatgggcggt tctgcagctc gctcagggat
     3481 gccagggctg aatctatgtc atgcaacata gggccaagaa cagacaaaag atgctcgccc
     3541 gcttcagttg gaacaacgct gcgcgtggtt cgcgtcagaa gccgcaatcc caatcgttct
     3601 tctatacgac gcactatctg gcttaaagct gactgcgcca tgctcaggcg ggctgctgca
     3661 cgagtgaagc tacgctcctc tgcaacgacg acaaacgcca tcagatcagc gatttcttca
     3721 cgcttcattc agtatctcca tgcaatctat taatcattta aatgataggt ctaatcatga
     3781 ttttgggtct aatcaactta atgatcaata tctatagtga tatcaccaac cgttaaaacg
     3841 aaccgacaac tccatgagga acggaatgat gaataataaa gtcagcttca ctaacagcaa
     3901 taatccaacc atctctttgt ctgcagtgat ctatttccct ccaaaatttg atgaaacccg
     3961 tcagtatcag gcaattgtgg tatctcaccc aggtgggggc gttaaagaac aaacggccgg
     4021 aacctatgcc aaaaaactgg cggaaaaggg atttgtcact attgcttatg acgcatctta
     4081 tcaaggtgaa agtggcggcg agccgcgtca gttagaaaac ccatacattc gtactgaaga
     4141 catcagtgcc gtgattgatt acctgacaac gctttcttat gtcgacaata cccgaattgg
     4201 tgcgatggga atttgtgccg gtgcaggata tacagccaat gctgcaattc aggatcgccg
     4261 catcaaggcc atcggtaccg ttagtgcggt caatattggt tcgatgttcc gtaatggttg
     4321 ggaaaacaat gtgaaatcta ttgatgccct gccatacgtt gaagcgggtt caaacgcccg
     4381 aaccagcgat attagcagcg gagaatatgc cgttatgcca ctggcaccaa tgaaggagtc
     4441 cgatgcgccg aacgaagaac tgcgtcaggc ctgggagtat taccacaccc ctcgcgcgca
     4501 gtatccaaca gcaccgggtt acgctacttt gcgcagcctt aaccagatta ttacctatga
     4561 tgcttaccat atggcggaag tgtacctgac tcagccgatg cagattgtgg cgggcagcca
     4621 ggcagggagc aaatggatga gtgacgacct gtacgatcgg gcatcaagcc aggataaacg
     4681 ctatcacatt gttgaaggcg caaaccatat ggatctttat gacggcaaag cctatgtggc
     4741 tgaagcaatt tcagtattag ctccgttctt tgaggaaact ctgtaatgaa acatattcaa
     4801 atccgcaata gtgatatggc ctggcatatt gctgccaata ttcagtttcc accaaatttt
     4861 gatgagtcaa aacagtatcc ggcgattatc agcgtccatc cttttggcag ttgtaagaag
     4921 cagacgtctg gcaatatcta tggcaaagca ttggctgaaa aaggctatct cgttctcgct
     4981 tatgacgcca gtttccaggg agaatctggt ggggagcctc gctggataga agatccgaca
     5041 cagcgcgtag aggatattag ccgtgttatc gattatgcag taactctgcc atatgttaat
     5101 gctgaacgta ttggcgtgct gggagtgtgt gggggtggtg gctatgccat taatgccgcg
     5161 ctgacagaaa aacgaattaa agcagtagtg agtattacgg gtgttaatat cgggcgtctt
     5221 tttcgggaag gtttcagcaa ttacgatccg attggcgcat tgaatgcaat ggcgtcgcaa
     5281 cgaaccaaag aggctcgtgg tggagaatta cagatcaacg aattacttcc cgccagcctg
     5341 gatgcagcaa aatcccatgg actgaccgaa cgcgatgttt atgaagcaac tgactactat
     5401 aaaacaccac gcgggcaaca accgggaggt gcgacaaaaa tgttgttctc acatgcacag
     5461 aaaacgctgg cctgggatgc cttcgcattt actgaagtgt tactgactca gccggtgatg
     5521 gtagtagttg gtgaaaaagt cggtgccttc ggtgcttatc gtgatggact ggaagtctat
     5581 ggccgggcga tggtttcgca ggatcgtcag ttagtttcat tacccgattt ctcacattat
     5641 gaactgtatg acaaaccgga agcagttcag gaggcgctgg aaaaagttat tccttttttc
     5701 aacacccatc tgggataagt ttcatgagaa atattatgaa aaatgtactg attcttggtg
     5761 ctggcggtca gatagcccgc catgtgatta atcaactggc agataaacaa accattaaac
     5821 aaactttatt tgcccgtcag ccagcaaaaa tccacaaacc ctatccaact aatagcaaga
     5881 ttattatggg agatgtactg aatcatgcag cactgaagca ggccatgcag ggacaggacg
     5941 ttgtgtatgc caaccttacg ggggaagatt tagatattca ggcaaattca gtgatcgctg
     6001 cgatgaaagc ctgtgatgtt aaacgtctga tctttgttct ttccctcggt atttatgatg
     6061 aagttccagg aaaatttggc gaatggaata atgcagttat tggtgagccc ctaaaaccat
     6121 tccgcagggc tgcggatgca attgaggctt ctggactgga atatacaatt cttcgccctg
     6181 cctggctgac tgatgaagat attattgatt atgagcttac ctcacgcaat gaaccattca
     6241 aaggtacgat cgtatcacgt aagagtgtcg cagcattaat cacagatatc atcgataaac
     6301 ctgagaaaca cattggtgag aatataggca tcaatcaacc cggtactgat ggtgataagc
     6361 cattcttcat gtaaatcaat tacataacta ccatcgtact gtactatgtg cagtacgatt
     6421 tattaaactt attaaggtga actgatcaat ccgatgcgat taacatgtgt agaaccagta
     6481 agaataaaat acgccatgta tataacatta cgacaattct aatatctgca catcaatgaa
     6541 aatgcaaaca acttccggca aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa
     6601 ggatttgata atccgcaaaa tttgcacaac actgcctgta atacttcctg aaccagagac
     6661 gatcatgacc gtaacccgcc cacgcgccga acgcggcgca tttccgcccg gaacagaaca
     6721 ttacggacgt tcattattgg gcgcgccgtt aatctggttt cctgcccctg cagccagtcg
     6781 cgaaagtggt ttgattctgg ctggcactca cggtgatgaa aactcttcgg tcgtcacgct
     6841 ctcctgtgct ctgcggactt tgacaccttc tttgcgccgc catcatgtgg tgctgtgtgt
     6901 gaatcctgac ggctgccagt tagggttacg ggccaatgct aatggtgtgg atttaaacag
     6961 aaactttccg gcggcgaact ggaaggaagg tgaaacggtt tatcgctgga acagcgccgc
     7021 tgaagaacgt gatgtcgtat tactgacagg tgacaaacct ggctccgaac ctgaaaccca
     7081 ggcgctgtgc cagcttatac accgcattca acctgcctgg gtggtctcat tccacgatcc
     7141 actggcttgt attgaagatc ccagacacag cgaattaggc gaatggctgt cccaggcgtt
     7201 tgaattgcct ctggtaacca gcgtcggcta tgaaacgcct ggctctttcg gcagttggtg
     7261 tgcagatctc aacttgcatt gcatcaccgc agagtttccg cccatctcct ccgacgaagc
     7321 cagcgaaaaa tatctctttg cgatggcaaa cttgctgcgc tggcatccta aagatgcaat
     7381 tcgcccgtcg tgaactgaag cgccggttcc acatctaccg ccagccaggt cggtccgtca
     7441 agatcggcga aactgacctg cggcaccagc ggtaaagcgg cgctaatggc acgagaggta
     7501 cacaacatgc agcccagcat cagacgaaaa ccttgtgcac gtgcttcagt cgccagcgcc
     7561 agcgcttccg tcagaccccc ggttttatcg agcttaatgt taaccatctc atagcgccct
     7621 ttcagcgcct tcagattgct acgagtatga caactttcat cagcacaaat cggcaacgga
     7681 tgaataaaat tctccagtgc cgcatcgtcc tgcgcaggaa gcggttgttc aagcatcgca
     7741 acgcctaaat ccgccaatag ctggcaacgc gccgccaacc cttctgcacg ccaggattca
     7801 tttgcatcaa cgatcagcgt ggcatcgggc acagcggtgc gaattgccac catccgctca
     7861 ctgataagat ggttatccag cttcactttc agtaatttcg cgcctgcctg ccagagtgtt
     7921 gatgcactat tggccatctg atcaggcgta ccgatgacaa ccgtctgtgc agtgataact
     7981 gtcccaggaa gcgttatgcc gatcaaatca gccagcgatt gctgctgttt tcgcgccgcc
     8041 agatcccaca atgcacaatc cagcgcatta cgtgctgcgc cggcagggag aattttttgc
     8101 aactcctccc gtgtcagccc tttctctaat tgcggtacga cgctcataat ttgcgccatt
     8161 accgaggcat cactttcccc ataacgcgga tacggcgtgc attcgccggt gcctttaata
     8221 ccctcttctt ccagttcaac caccaccacg cgcgcttcac tgcgacttcc ccgggcaatc
     8281 acaaacgggg tatgtaaggg ccaggcttcc tcaaatacct taacggttct catctttact
     8341 ccttgttgtc gcggtggtct gcggattaag cgcaaatagc gtttgctgtg ttattgacag
     8401 ttagcataaa ctaggtgtga cgttaactat atgtaaacag gaaagataat catgtcacaa
     8461 accgttcatt tccagggcaa cccggttaca gtcgccaatt ccatcccgca ggcgggtagc
     8521 aaagcgcaga cttttactct cgtggcaaaa gatctgtctg acgtcaccct cggtcagttt
     8581 gcgggtaaac gcaaagtgct gaacattttc ccgagtattg ataccggagt ttgcgccgca
     8641 tcagtacgta agtttaacca actggcaacc gagatcgaca acaccgttgt gctgtgtatt
     8701 tctgccgatc tgccgttcgc ccagtctcgt ttctgcggcg cagaaggtct gaacaacgtt
     8761 atcaccctct ccactttccg taacgctgaa ttcctgcaag cctacggtgt ggcaattgct
     8821 gatggcccac tgaaaggtct ggcagcacgt gccgttgtgg ttattgacga aaatgacaat
     8881 gtgattttca gccagctggt ggatgaaatc accaccgagc cggattacga agcagctctg
     8941 gctgtactga aagcataatt taatatgcct gatggtgttg caccatcagg catattcgcg
     9001 cttactcttc gttcttcttc tgactcagac catattcccg caacttattg gcaatcgcgg
     9061 tatgtgaaac gccgagacgt tttgccagtt tgcgcgtgct gggataattg cgataaagct
     9121 gggttaatac cgagcgttca aaacggctgg tgatttcgtc cagcgaacct tccatcgcat
     9181 cttcgcccac ggctaccgtt gcggcgtcat aatccggcaa caaaatatcc tgtggacgca
     9241 gctcataacc gtccagttgt gtcagtgcgc gatagatagc gttctttaac tgccgcacat
     9301 ttcccggcca cgcataacgc gtaagtacag tattcaggtc agcggccagt ttcggacgcg
     9361 gcacgccctg ctcgtcggca aagcgggcga cgaacagctc agttaacggc atgatgtcct
     9421 gcggacagtc acgtagcggc ggcagattga gcgtcaacac gttcagacga taatagagat
     9481 cttcacggaa catgcctttt tgcaccagtt cgaccagatt cttctgcgta gcgcaaatca
     9541 cccgcacatc gacatgcacc tcatggtctt cgccaacccg acggaaagtg ccatcattaa
     9601 ggaaacgcag taatttcgcc tgcatccgtg gtgacatctc acctatttca tccaacagca
     9661 ccgaaccacc gttcgcctgc tcaaagaatc ctttcttccc ttccggagca tgaccaaaca
     9721 gttcactctc gaccgcatct tccggtatag acgcacagtt cagcgccagg taaggtttgc
     9781 ccgctctggg gcttgcctga tggcaggcgt aggcaaagag atctttacct gtacctgtgt
     9841 cacccgtaat cagcagcggc gcgcttagca tcgccagttt ctgcgcctgt tcgacaacgt
     9901 gcttcatttt cgggctgacg gcgacaattt gactaaaggc gctgacgtcc tgggctgcga
     9961 cattttgcaa ctggcggccc atacgaatcg ttgatcgcaa catcaccacc gcaccggtca
    10021 ggacgtgttg atcattttca tcctgaagat aaacaggcgt aatctccatc aggaaattct
    10081 gcccattaat aacgacatgc tcgttatggg aatcttgcgg ttcgctttcc agccaacgta
    10141 aaaaattaaa gccgttaatc aattgtgcgg cggtatggtt gcgcaggcga tccaattttt
    10201 gcccaaaaag ctgacagctc gccgggttcg ccatatccac tttgcttttc atatcgacag
    10261 agagcacagg ttcgggcaac gcctccagta acgcgctcaa cgccagatgc tcacgttcgg
    10321 aaggcatcca cgggacagta cgcacatcgg taacacctgc aatacggcgt atttcggcca
    10381 tcagactgct gaaactctca aactccagtt cagcaaaatt gaggtagatt cgcccaatgg
    10441 gatcaatctc aataccgcgt aaatcaatgc ctcttagcac gagtagatcg agtaattcgc
    10501 gggtcagacc gagtcggtct tcacaaaaga cttccagacg catgggaacc ttcacctgaa
    10561 aaaagaacaa ttaatatgat gatatgacac tatttggata gcaggaaggt ttctgtcaac
    10621 aatcattgac acaaagacgg aaaaacgggc acttcggcgt aaagatatcc ggtcagcacc
    10681 cgatattgca ttatttttcg ctgggcgttt tgcctttttg cagcgtttct ttcacctgac
    10741 cgataagctg acgacggaaa tccccgaggc gaggtttgtc atcgtcaatc cacggcagcg
    10801 ggcggcaaag ctccatagct ttaatcccga gtcgtgccgt cagaagtcca gcaccaattc
    10861 cctgagctgc gcgggtagac aaacgagcag cgagatcttg cgacatccag tccatcccca
    10921 cttcgcgcac cagttcgctg gctccggcaa aagcgatatt cagcaatacc agcttaaaca
    10981 gacgcaaacg gctgtaatac cccagttcaa tgccatacag cgtggcgatg cgattaatta
    11041 aacgcagatt gcgccaggcg ataaacgcca tatcgaccaa cgccagcggg ctgaccgcaa
    11101 tcatcaacgt tgattccgcc gccgaacggc tgatttcgcg ccgcgcctgg gcatctaaaa
    11161 ctggctggac caaatgcgca tacaaactga ccacttcacg gtcgttttgc gtttcatgga
    11221 ttgaggcata ccagcgttgc agcgctggat gagactgatc aatacccgcc tgctgcgcca
    11281 gtttttcgca aaatgcgcgg cctttgcccg tgccgtggct gtgcaacaaa tcgcgcgctt
    11341 cgtcgcgttc atgggcgcgc tgtcgcaagc gccataagcg ccgccactct gttaccacag
    11401 aacctacgcc agcgccgata atcaatgccc cagcggcaca tccacccagc gccacccagt
    11461 cctgagtttg ccaggcattc attgtccact gtacaccctg cccgacaacg cttgcgccaa
    11521 acagagccag cccgcccatc accattttgc gccacaggct gcgtttcgga cgtaacgctg
    11581 catccattac cgcttcaact tgcccctctt cttcaggcgc ttcatcgagc gtggccgggg
    11641 caaaattttg cgcctgattt tcgtcaaagg tctgctgcgc cctgaatttt ggattctgat
    11701 cgacctccag cggaccgtcg aaatcaatac gtggttttaa cggttcggtc atcgcaattt
    11761 atctcctatt aaaaattcaa gcgcggcatc aagacgaata tgcggcagcg gtttgtcgac
    11821 atccatcacc tgcgggcgaa acgcttcaaa ctggaaccct tgcttgtccc agaacgcctg
    11881 accgggcaaa cgcgcaggaa cttcaccggg ataaacggtg agtggcgcac catcgctgag
    11941 ccggttaccg cgcaatgccg gaattttctc gccgttgacg tcaataatgc cgctggtggt
    12001 cgcctgcacc gaggccagcc ccaggcaatc catactaatc ccttcaaacg ccgcattttg
    12061 ccaggcatcc tgaatcagtt gttgcagtaa tgaaaccata ttggcgtgct gatcgatggt
    12121 cacatggtcc gctttagtgg cagcaaacaa tagcttatcg ataaccggcg aaaacaaacg
    12181 ccggaacaag gtacgctgcc cgtagtggaa actttgcatc agctgcgtta gtgccagacg
    12241 catatcatta aatgcctgtg gcccactgtt gagaggttgc aggcaatcaa ccagcacaat
    12301 ctggcggtca aagcgcagaa aatgattctt atagaacccc ttcaccacct tctcgcagta
    12361 ataattaaac cgctcgcgca gcattccggc attggtgtgc ttatcggcct gcgccagttt
    12421 ggactcgccc caggcatcga catccggcca cgggaagaat tgcagcgcgg gcgcacctgc
    12481 catatctccc ggcaagacaa agcgcccagg ctgaataaag tgcagcccct gctgtttaca
    12541 gtggtggaga taatcggtcc acgcggcggc aatgtctgcc agccggtttt cgtcggcagg
    12601 cgctagcggg tccagccctt cgcacatcat tcgccatttg accgaccatt cgccgcgctg
    12661 accattgagt aagcccgtca tctggcgtga ccagcttaaa tagtcctgcg ccagcatcgg
    12721 caggtcgagc aaccattcgc cgggataatc cacaatctcc agatacagcg tggaggtgtc
    12781 cttgaagtgg cgtagcagcg aatcgttcga tttaaaacgt agcgccaggc ggatttcact
    12841 gacgccgcgc gttggcgtcg gccaggcggg aggatcgcca tataactgcg ccagtccttc
    12901 atcatatgtg aagcgcggaa tgccaaagtc acgctgagga atgcgtttta cgcccagcag
    12961 gcgctcttca cgcaccgcgc ttaacagcgg caagcgtgct ccggcgtgaa tattgagcaa
    13021 ctgattgacc atcgcagtga taaacgctgt tttgccgctg cggcttagtc cggttacggc
    13081 gaggcgcaga tgtctgtcga caccccgatt caccagcgca ttaagttcat ttttaagtcg
    13141 cttcattgct gtcctgtgct gctctgaaga ccagaaaaaa atcattcgta aggatatgaa
    13201 tgtgggggcc aggccattga ttatcaactg cccgtgttgt catcgacggg cgaactccat
    13261 aagatgtgca gcattcacgt tcggacggag ttcctgacct atgtccccta ctatttatga
    13321 tattgccagg gttgcaggcg tatcaaaatc caccgtatca cgcgtgctga ataagcaaac
    13381 taatatctcc ccggaagcgc gcgaaaaagt gttacgggcc attgaagaat tacagtatca
    13441 gccaaacaag ctggcccgcg cgctgacctc ttcgggtttt gatgccatta tggtgatttc
    13501 tacccgttcg accaaaacta cggcgggtaa tccgtttttc tctgaagttt tgcatgccat
    13561 caccgccaaa gctgaagagg aaggtttcga cgtgatattg cagacgtcgc acaatccggc
    13621 agaagactta caaaaatgtg aaagcaaaat taagcagaaa atgattaaag gcattattat
    13681 gctaagttcg ccagcggatg agtcattttt tgcccaactt gataaatacg atattcctgt
    13741 cgtggtgatt ggcaaagttg aaggtcaata tgcccatgtt tattctgtcg ataccgataa
    13801 ttttggcgac agcattgcgt tgaccgatgc attaattgaa agtgggcatc aaaatattgc
    13861 ctgcctgcat gctccgcttg atgtccatgt ttcggttgat cgcgttaatg gctataagca
    13921 aagtctgtcc gcgcataata ttgcagtgcg tgataaatgg attgttgacg gcggttatac
    13981 ccatgaaaca gcactgcaag cagcacggca attattaagt cagtcaccgt tgcctgaggc
    14041 agtgtttgcc actgacagcc tgaaattaat gagcatttat cgtgcggcgg cagagaaaaa
    14101 tattgctatt ccgcagcagt tagcggtggt gggttatagt aatgaaacgc tgtcgtttat
    14161 tttaacgcct gcaccaggcg gcatcgatgt tccgacgcag gagttaggtc aacaaagctg
    14221 cgagttatta ttccgcttaa tttctggaaa accgtcacca cagaatatta ccgttgccac
    14281 gcatatgacg ctgaaataaa taccagctac cttgcctgaa tgccggatgc gatgctagcg
    14341 catcttatcc ggcctacatt tatagatcgg ataaagtact cgcgccgcat ccggcaattt
    14401 agcccattat cagaacgagt aatttacgcc gacacctgca taatggaatt tatcgctgtc
    14461 gccttcgtcg tgatcctgcc actcaaacgc gtattccagc gaaacggaaa gaccattctg
    14521 gaaatcataa ccgtaaaata aacccacacg gttaaaatca tggccttcac gttcgatatc
    14581 atcctgccag tcccagttac tccagcgatc cagcccaatg cgcgtatacg gcgtcaccga
    14641 gtggttgccg agcgtcagcg gcaaataggc gcgaatttct tgcgtagaaa actcaccgtt
    14701 attgcggctg tcgtccatat taaagccgcg ctcgagataa tagttcactc gcaaggcaac
    14761 cgtttcgttg aaggtatatt gcaggcctgt ttccgtttcg actcgggtat cagcgtaacc
    14821 ggtcgtgttc agatcgttgg caaatttata catcgacaac caaccgttga aacgtaaatc
    14881 gtcagtcagt ttcacatccc agtctggcgc gattttccag cgctgcatat tcgccgtgtc
    14941 tttacccggt tcatcaacgt agtgataacc ataattacgg aaaccgccgg tcaggccgaa
    15001 actgaaatca tcgttttcga ggaactgata atgcacctcc agctccgggc gatcaaacca
    15061 cgttccacgt ttacccgcgc tataatctac cggcccttcc tgataatagg ccagagcaat
    15121 tctccacggc ccgttggcgg cattaaaata gactgaaggc tccgccagcc catccatatc
    15181 ttcgccataa ccctcgacgt tttctatttc gtacatcgcg ccgatattaa agtgccagtc
    15241 gttcctttcc tcggcctgtg cgcaggccat tcccgcacac atcaccagtg cggtacaggg
    15301 taataacttt ttcattattg ttccttgtat tattctgggg gaatgccttc cccctgtatt
    15361 tagcgaattg ctatttccgt ttctgcatca aagaaatgac atttcgtcat atcaaaatga
    15421 atagtgatat tttctcctgc atgataatca tttaacgcac cagcacggac cactaactcg
    15481 tgccccccaa ccgtggtgta gagcataaat tccgcaccgg ttaattctgc cacgctaatt
    15541 ttggcggaaa tgttattttc ctcttgcgcg tccggatgaa tatcttccgg acgtattccc
    15601 atcacgatgg gcttatgcaa acttccctgt gttttcagaa ccgctaattt ctcttcggga
    15661 atggttaatt taagcgtttc cgtaacgaat ttatcgccat cgatcgtgcc acgaataaaa
    15721 ttcatcgccg gtgatccaat aaatccggca acaaacatat tcgctggttg gttataaacc
    15781 gttttcggcg caccaacttg ctgaacaatc ccatccttca taatcacaat ccgcgtcgcc
    15841 atggtcatcg cttcggtctg atcgtgggtc acgtagatca tggtggtatt cagcttctgg
    15901 tgtagcttgc tgatctctgc gcgcatttgc acgcgcagct tggcatcaag gttagagagc
    15961 ggttcatcca ttaaaaacac gcccgcttcg cgcacaatcg cccgcccgag cgccacacgc
    16021 tggcgttgcc cgccggaaag cgcgcccggc ttgcgtttca ggtactcacg caggccgaga
    16081 atttgcgccg cccagttcac ccgctcatca atcacctctt tggcgatttt ttgcatcttc
    16141 aggccaaacg ccatgttgtc gtaaaccgtc atatgcggat acaacgcgta gttctggaac
    16201 accatcgcta tattgcgtgc tttggctgga acgtcattca ttcgtttgcc gtcgatcaac
    16261 agatcgccgc cgctgatctc ctcaagcccg gcaatcatgc gcagggtggt cgacttaccg
    16321 cagcccgacg ggccgacaaa cacgatgaac tctttatcgg caatttccag gttgaagtcc
    16381 ttcaccacat gcacctggtt atcgtagatt ttttgaatat gttgtaacga aagctgagcc
    16441 atgttgattc ctttgctata cgttttgcca gaaggccgat aaccgcgacc aggtcagaga
    16501 gtccgttgaa ggcaacagta attgcgcccc ggttaagccc gcgccgatcc ccaccgagcg
    16561 cataccgctg gcgttaatgg cgtcaatgcc cgcctgcgca tcttcaatgc cgatacatgc
    16621 ctgcggcggc acgcccagcc cggcacaggc agcgagaaag atttccggat ccggtttcga
    16681 gtttttaagc tgggaagcat ccgcgcagaa ggtgaaaaac tcgcgcagct ccagcgccgc
    16741 taaaatcgtc ggcgcattca gggagacgga agccagccct accggaatct gctgtgcacg
    16801 gagctctgcc agcaaattgc gaataccggg cagcacagca ttaaccgtca gctcgcgcag
    16861 tgagtggacg taaagcagat ttttgcgata cgccagttgc gcccgctcct gcgggttaaa
    16921 gtcgccctct ttgcccccgt gttgcagaat gcgccgcaga gactcatcgc ggctgatccc
    16981 ttttagggat tcgttaaact gcgcatcaat gctgatgcca atttcagcgg caatctgctg
    17041 ccacgccagg aaatgcagat gcgcggtatc ggtgattaca ccatccagat cgaaaattac
    17101 cccttgcagt ttcattcacc ctcctgatgc gtggtagcgg tcccattgaa gggcaaaata
    17161 aaatcaccca aacagaaaac agattcttca gccacggata taagctgacc gttcaaacgc
    17221 agtgaaacgg gcgcagaagt tcgaatcgca atacgctgcg cgtcgagagt gacctgtaat
    17281 tcgcagccct gccagaacag agggaaagac aactgttgcc actgctcagg taacgccgga
    17341 ttgagatgca attcaccgtc acgcacgctc accccggcaa aaccctgaat cgcccccagc
    17401 cagatagcgc cagttgcggc agcatggata ccatcatcac aactatgcgg atcagcacca
    17461 agatcgattt cagtcccctc gcgccagaac tgataacttt gggtcagcag gccacagcgt
    17521 gcggcaacaa tgccgtggat tgctttactt aatgacgagt cgtgaatagt gcgcggttca
    17581 taaaattgca gattggcaag acacgatgcc gctgagaact gctccggcag catgtaattg
    17641 agcatcacca catctgcctg cttgaggatc tgcatctcgt tcacttctgc gcgtgaatat
    17701 tccagcagta tggtttgctt ccccgccgcc gctttgtatt tcgccaggtt aatcgccggc
    17761 ttagccataa acgaatcatc ctgcggcaaa acgccgtcgg gctgaatttc tggcagccgc
    17821 agttctttaa ggaacatttc ggcacgatga ataaacgcgt cgtcgctaca gccaaactgg
    17881 cgggcaatac tcagcgcctg ctggacgttg taatacgcca tatagctggt gaaggcgtta
    17941 ttattgacat gttcggtata ttcgtcaggc ccaataacat catgaatttc cagacggtcg
    18001 ttaaccctca ccgcgcggct aatccagaac tttgcagttt ccagaagtag cgccatgcct
    18061 tcatgcgcaa tgaaactttc atcccccgtg gtctgccagt attgaataac cgcccaggcg
    18121 atatcggcca ccagatgatg ttccgcctgc gccgaggcca ctttttgccg cagcccggtg
    18181 cgaatgttaa tggcggcaaa aagtggcgtc tcttcttcgc cgctgcgcgc gctttcccac
    18241 gggaatagcg cgccctgcca gccattgcgt cgcgctttct cctgcgcgcc aggcaagttg
    18301 tgccagcgat aacgcagtaa acttctggca atcgtcggat cgctaaacag atggaacggc
    18361 agcaaaaata cttccgtatc ccagaaaacg tggcctttgt agccttcgcc ggttaagcct
    18421 tttgccgcaa tgctgctacg ctcgtcgtga gcaggcgtca tgatgcgcag atgataaagc
    18481 gcataatcta acgcttgctg atcgtgcgca tcgccgccgt ttaccgtgat acgacgtttc
    18541 tgccaccact gacgccagtt ttctgttgat gctgcaagaa gttggtcgta actctgctgc
    18601 gcgcacattt caagctggcg aagcgacgcg ctgccccact cgtctaaaac ggcttgcctg
    18661 tcatcccgcc agtcgatcca caccagtttt tgcaacgtca ctgtctcgcc tgcatgaagc
    18721 tgcgcactgg tatgttgctg caaacggcgc tctttggcgg tatagcattg ctgcacatca
    18781 ccgctcacca tacagcaaca gctgatggcc acatcactgc gtccatcctg ggtggtgtag
    18841 atcccctgca tcagatgctg accaaacacc cgcacctggg tttcgtcgag atgttggcga
    18901 ccgtggttgg tttgcgtggc gtcgatacct gttgaaatca gcactgacgc gtcggcgtcc
    18961 agtggcgtaa tagtgatttc cagcacaatg agcggcagtt ggtctgccga aacaaagcga
    19021 cggctggcga tggtgtaacc tgtgccgttg ctggtacgcc agacaacgct acggcgtaat
    19081 tctccactgg caaagtcaag ttcacgctgc caggcttcgc gggataacga gaaaacctca
    19141 ccatttatgg caatctccat ccccaagata tcaggcaggt tcaccagttc gttgatttca
    19201 ccttttcccg cccgatgata cagccccgcc agatacatcc ctcgcgtctg gcgcgtgtaa
    19261 tcttcttcat ggctggcgcg aagcccaaga tagccgttcc cctgcgccat cagcgatgca
    19321 tacttgttca gggtatgctg gctgaaatgg ggttctgata acgttactgg cctggtcata
    19381 attccacacg cgtccctgtt tctgcggatt gatacaacgc cgccaccagt tgctggatga
    19441 tgtacccctg ctcggcgtcg gctatcatca cgggcttgcc ctgtacgtga ttgataaagg
    19501 cttccatgct gcgcaaatgg cggttgtcgt ctgccagttc ccgttgcatc agcgtcatta
    19561 attcaccgtt gttatcggtg tagatatgtg ctggaaacag cgtcgcaccg gctttatcac
    19621 cacaaaagct gacgttcata atcgactgtt cgcggatgtt gagtgcaaat gacgtttcca
    19681 gccacagaat gccgccgtta tgaaattcaa tggtgccaaa cagcgaatct tcgacgctat
    19741 aagttgccgg atcccactcg ccaaattgac cacagctctt ttgcgtgccg atcttttgaa
    19801 agctatgcgc attcacgctt ttcaccgccg gaaagcccag cacatacatc gcagcatcca
    19861 gcatatgaat gccgatgtcg atcagcgggc caccaccctg cagttcttta ttggtaaaga
    19921 cgccccagcc gggaacgccg cagcgacgca gggcgcgggc ggtggtaacg taaatctctc
    19981 ccaaaacgcc gttggtcacc tgttcacgca gctgttgcgt atcgagcgca aaacgatggt
    20041 gaaagtcgta ggccagcacc ttgcccagtt tgcgcgcggt atcgcacatt tcccgcgcct
    20101 gttctggcgt catggcgggc ggtttttcgc acatcacatg gcagcccgct tccagtgcca
    20161 tcagggtatg ttcgtaatga aaacggttag gtgagcagac gctaaccaca tcaggtttca
    20221 ccgccagcag catggcctgc ggatcgtccc acacggatgc attcccgtat ttttctgcca
    20281 gcgcctgcgc ctgggaaagg cggctgtcac agacagccac cagctccaga tcgttgcggg
    20341 tgcagtagta cgaagcatga accttatccg ccacctggcc tgcgcctatt atcgcgaccc
    20401 gcagcggaga gcttgtcatt gcacttttca caaagacctc ttagcaggta cgcaaccagg
    20461 ccaacgaatc acggtacgcc tgggcaggat cttccgcccg gatacgccct tcgtacacta
    20521 catagccctg atagttatcc gcgcgcagct gttcaaacag cgcgtggaaa tccagggtgc
    20581 cgctgcccgg ctggtagcga tggttatccg caatatgcac atgaccgagc aggtcgcggt
    20641 tgtcatgcag cgcctgcgcc aggttatctt cttcgatatt catgtgatag aaatcgccga
    20701 taatctgtac atgtttaaga tcgttttcga cgatgtaacg gcgggcatcg gcgagggtgt
    20761 tgatcatatg atcctgatag cggtttaacg gttcgagata caccacggtt ccggtacgcg
    20821 cggcgacctg ttcaagtacg cgcagggaat cactcaccat tttacgatcg ccgttcaggc
    20881 tgcgcggcga ggtcatcggc ggtaagcgga aggtaaacat gccccacgct gccggaacgc
    20941 cgataccctt accccccact tctgccagcg cttcgagaat gcgttcgatc tgctttaagc
    21001 cattaagacg acgctcttca ataaagtcac caatccagcc gtcatagcca ccgcaggcgg
    21061 tggtcaccgg taaaccggtt tctttgattg ctgctttgac ttcttcgatg ttgttgacca
    21121 gcagtttgcc gtcaatctca aaaccatcga agcccatctc tttgatataa cgaaatttct
    21181 ccagaatgtt ttccggaaag aatgcctgat tctgtgtgcc tattttcatt ttttcattcc
    21241 tttgaattaa aacgtaacgc ccattttgat gctctgttcc ggatgctgat cgacatactg
    21301 catgtagctt tccgggctat tggcaaaggt cactaccggg tcgattaaat cttcgcaatt
    21361 gagataaccg ttcatcagca gttcccagca ggtttcttca atacgcttac ggctccagcg
    21421 cggataatcc gggttcggtt cgctgcacgc gcgggagaaa acaattttgg cgttattgaa
    21481 atgcgcttcg cgtccgaggt taaaaccttc agcaaatggt ttggcaaacg cgacatagga
    21541 gatggtgcca ccgtaggcca gaccgcggaa cgccgattgc agcgcgtcgg catagccgct
    21601 ggtttcgata atcacatcgg caccctgctt gccggtcagc gttttgatct ctttgcctac
    21661 gtcagtacca atggggttaa ggcagaaatc cgcgccgtgg cgacgggcaa tatcacagcg
    21721 atgggcgata ggatcgacgc caatcaccac cgacgcgcca gcgcgtttcg ccagttgaat
    21781 ggcaatttga ccaatcgcgc caagccctac caccacaaca aaatcaccca cgcgcacgtt
    21841 ggcatcgcgc acgccgctca tggcaaactg cgccgggtcg taacaaacgg cgtttttcca
    21901 cgagctgcct tccggcattt tgcgcagctt gtagttattc accgcgttaa tgatgaccgt
    21961 ctcggagagc gggccgtagc cgcatacgct gtcgcccacc gcgtagtcgg taacgtcgct
    22021 gccgcactcg ataatgtcgc caaccaccat gtttccaagc tggaatttgc caaactcaat
    22081 gccgcgcggc gcatctgccg gacgcggagt gaacatctgc cattcgccgt taaagtcttc
    22141 atcgataaac gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt tcggtgcgcc
    22201 gaaacgcacg cggatcttca cttcattggc taaaatcgcc cgatcttcat actcaaccag
    22261 cgcagcaaca cgcggtgctg tggctactaa ctttttcatt taaaactcct tgtaaagaaa
    22321 ctggccgctt tagcccttca cgccaccggc ggtcaaacca ctcttaataa aacgttccga
    22381 aagcgcatac ataatcacca ccggcaatgc ggtcaccagt gaggccgcca tcatccgtcc
    22441 ccagatgtaa tctggcgtac tgaacagcgc gttcaggccc accggtaagg tgaaattgct
    22501 ggcgctggag aggaagatcg acgcaaacag atagtcgttc cacgccacca taaagcagta
    22561 gacgaacacg gatatcagcc cggacatcgc caacggcacg gtgatgcgga agataatttg
    22621 cagacggttg aggccatcca tcatcgccgc ttcttcgatc tcatcgggga tggtgtcgaa
    22681 gtagcttttc agcataaaca cggcggtggg cagagtttgc gtcaccatgg tgatgatcag
    22741 cgccatctca gtgtcgtaaa tgcccagcgc ggtgatgatt ttgaacagcg gcaccaccag
    22801 caaaatgcca gagaacatat acaccgtgta aaagctggcg ttgatggtca tccgaccttt
    22861 aaagcgcagg cgggaaagcg cgtaagcccc aagaatgccg aggaataccg ccaccacgga
    22921 tgaaaccaca gacaccacca gactgttacg gaagtagtcg acaaacggga aaatcatcgg
    22981 gttaaaaatg tcgacgtaat gctccagcgt ccactgctgc ggcagcagcg taggatgcag
    23041 tgagatcgcc tctttcgcgc ccttgaacga ggtcatcagc atcacaaaaa atggaaacag
    23101 cgtgatgatg agaaacagcg ccagcccgca gtaaaaaccg atgcgactga gtgtgcgttt
    23161 atttgttgcc attgaggttc acccgtttcc tggtcagcag aatgacagcg aaaataatga
    23221 tgaagagcac caccgagatc gccgccgctt ttcctaaatc attaaaggca aaggcggttt
    23281 tgtagagata tacaccgaga atatcgacct tggtcgtcag caaataaaca tccgcgaaca
    23341 tgtagaacat ccagatggtg cgcagggtca ccaccgtcgc caggaccggc ataatcgcgg
    23401 gcagcgtgac gatacgaaac cgttgccagg cattagcgcc atccatctct gccgcttcgt
    23461 acagcgattt gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca tacgggaagt
    23521 agcgccagat ggcgaacagc accaccagca caaagctact gcccggatta tcgaaccaca
    23581 gcggtgcctg ctcatagaga tgcagaagat cgacgccgag gtagttcaca atgccgtagc
    23641 cgttgttgaa catgtatttc caggcaaaca ccagcgaaat ggacggcgtt acgtaggaga
    23701 ggatcaccag cgaacgcgcg gttttgcgca gacggaactc gcggttgaaa aacatcgcca
    23761 ctgccagccc gagcacggtg ctgcccgcca ccaccagcgc ggtataccag accgtcatcc
    23821 acagcgagtg ccagaagccg ggatcggaga ggatacgcac atagttactc accccaacaa
    23881 aggttgactc gatgttggga ttgagcggca gacgtaaaaa actgatttcg atattcgaca
    23941 ccattggcca ggccaccaga ccacccagca gtaataagct gggcgcgaga agcagcagcg
    24001 caaagggcat atcggaacga cctgaaaaaa gcctgttcat tgcggattcc tgtcctgggt
    24061 ttagtgctgt tcgatcagtt catccagctt tttctggctc gcttgcagcg tgccagggag
    24121 atcggcttta cccacggtga cgttatgcac catggagctc accacgccag accccgtcac
    24181 atcacccatg cgggtaaagt ttttatcccc tactgcacca aagacctgaa tatttggcag
    24241 ttcaccgatt agctgattcg gtagttcacc cagcgcctta ataacgtcgt tgtctttcca
    24301 ggtggcggta gtgacaaccg ctttattcac cggcagcgca gcacctggcg acatcatcac
    24361 ccagtcggca atgttgtctg cctgctccat aaaggtgaca aatttttctg ctgcttccgt
    24421 ctcttcggtc ttttgcccgg cggtaatggt cagcgaggtc aacatgccgt agaccgcaga
    24481 gtttttctcg gttggcacca cgaaaccgac gtttttcggg tcgccttctt taatcacagc
    24541 cggaaggata taggtggagt aaatcgccat cggcgcggtg ccgttcataa aggcgtcttt
    24601 cacttccatg atgtcgttag aacccggcat agtgttggca gcaaggtcgc ggtaataggt
    24661 cagtgcctgc atcatctctg gtgtatcaag ggtgattttg ccttcggcgt taaagacgtt
    24721 agcctggttg gataacgcaa actgggagaa ggattgttcc gtcaacacgc tttctgctgt
    24781 aggcagcgca atgccgtatt ttttattcgc cgggtcattc agtttctgtg caacgtccag
    24841 cagctgttgc cagtttttcg gctcctcaag ccccgctttt gccagcacat ctttgcgata
    24901 ccagataccg ccaatccagg cgctgacagg aacaccggtc catgcgctac catcttcggt
    24961 acgcacaata cgcagtacgc catcgtaaaa cgcgccttca ccaacgttgc tgatgactgt
    25021 ggcaaccgct ttgcgatcga taagctgctc tttgtccatc actttggcgt agtcatggct
    25081 ggtttctatc acttccggca gcgaaccgct acgtgaaaga gtaatgactt tagtgttata
    25141 ggcatcttct tccacgggca cttgcttaac ggtgatgcca gggttttctt tttcaaaacg
    25201 ggcaatcaat ttactgataa cggcctggcg ctcctgctcc accgaagaat gcataaattc
    25261 gatggataca ttcgttttat tttcttcttt acagcctgaa atcagggcgc aggaaaccag
    25321 tgctgataac agcacgattt ttgatttaat catgagatgt tcctttttat ttaatccaca
    25381 taacctgcca ggggcgcagc gttatttcat tacccaatat atcaacttca ctaatcagat
    25441 cccgaccatg cagattagta atatttacat gctgaatatt tttactgaca ttaaacaatc
    25501 cagtcagaca attaccatca gcgttacttc tttgaatacg cattacggat gaattaatcg
    25561 tatcgatggt aaaattatta tccggatgaa actcgttatg gctgcgacga agtgtaatta
    25621 aacgcgacaa ctcatgatat accgcatgcc ttaatgtagc ttcatcgttc agttctcggg
    25681 ttatctcttt actgtgatat tttttacggt taatcgcacg gttatatccg agtttttcga
    25741 cacctgcgta atcattacgc gagccaagaa tactttgaat atatatcgct ggcacaccgg
    25801 ggaaacttaa caaaatcgca tgggcgagaa taaacctggc gcaacgttct tcatcgctac
    25861 tctcacggcg gcttaacgca tccatatagg tcacatttat ttcatacgga ctgcgtgtac
    25921 cgtcgggatt atttttccag tttactaatg caccttcctg ctgtaacgcc tcgaccagct
    25981 ctaatatttc gctttcaggc aacaagcccc gtagcgggtt taacccgatg ccatcgtgag
    26041 aggcgaggaa gttaaaccag gtggtgttgc tggaaggtag tgtcaggttt tgcgcccacg
    26101 cacaaagcgc ctcaacgttc tgtttttgca ccgcatgcag caccagtggc ggcagcgaga
    26161 actggtacac catatgtgct tcgtcatcgc ctgcgccaaa gtaagcaatg ttgtctttat
    26221 gcggaacatt ggtctcggta atgatcactg tacctggcgc aacgttatca ataatcgacc
    26281 gtaacagttt gataatcaga tgtgtttttt ccagatggat gcagcttgtt cccggctctt
    26341 tccacataaa gccaacggca tccaggcgga catactcagc acctttcgca aggtaacaga
    26401 gtaaaacatc caccatcgcc agcaacactt cagggctacg gtaattcagg tcaatttgat
    26461 cgtcactaaa ggtggtccat aaatggcgcg ttgaatgatc gcgcatctgg aatggcgtta
    26521 ataacggtaa cgcacgcggg cgagtgacgg cgctgagatc ggtttgcggg tcaacggcaa
    26581 taaaaaaatc ttcaaaacct ggatgctgtt gtaaatagtt tttaaaccat tcactttttg
    26641 ccgacatatg gttgcagaca aaatcaaaca ttaaatgact gcattcaccg agttgctgaa
    26701 tatcctgcca ctcccccgct tcactggcga cctgttgata atcaattacc gaaaagccat
    26761 catcagatga ccatggataa aacggcaaca aatgaacatg tgaaaaaatg ctttgcagcc
    26821 attgatggta aaactgatta aatgtgggta atggttttaa atcattgctg tgaaattgat
    26881 cggcataggt aattaacacg acatcacttt catcccagtg ttttttacgt cgctgtgtaa
    26941 ttaatcgttt cgcactctca agacgcgtta caagtttctg taaatgagtt gcggtaaatg
    27001 ttccaccgta gatttcgtcc aggtgatccg taattttctg tttcatgttg cctttccgtg
    27061 gtagcggtcc catgaaacga gagactactc cgcagaaaat aaactgtcaa ttgagcacaa
    27121 gggttttagt cacgcaggaa atcatttttt accagggaaa aagcgtattt gtgagctgac
    27181 gcaaataatc tatggggatg taaataaggt gtgacagaaa aaaaaacggc gcataagcgc
    27241 cgctcatggt gaattctttt aacctttgac cttcggcatt gcgatgtctt tcaggccacc
    27301 ggcgttctca acgtgggtat atcccatctc gctaaggatc tcttttgctt gccctgactg
    27361 gcgtccggca ttgcaataca ctttcacggt gtcgttttta tccggaacgg cggtggcaat
    27421 gcgctctttc acttccttca ggggaatatt gatggccccc tgaacgtgct cttgctgata
    27481 ctgctctgga acacgaacat cgatccagtg ttcagcggcg aaaacgggca gtgaaaacac
    27541 cagtgccaga gcaagtaagc cttttttaaa catgtaagcg tccttctatg gatgaaaatt
    27601 gccatccatt atacgcctga gatttaacaa tttttgcgaa actcccctca ccttttataa
    27661 cgctgtgcca gtttattagc agcccgactt aacagcggtt ccagcgccac agccagcaac
    27721 attttcagcg gtcggcgagc aactgatttt atcgcccagc ccgccacacc cgccgggcca
    27781 tagcgcaatg cggtaagaag taccagcttg cctgctaatt tgaaaccagg ctttaccttt
    27841 tgcccggcct gttgccagcg agtattcata actttcctca cagttgacgg aaacggctac
    27901 gtaacgtgaa agtatcggaa gtgacataac gttccatctc gcgtaaacgc gtttcacttg
    27961 ccgccagttc acggtcgact tcatccagca attcgctgct ggaaggtagc tgctcaccaa
    28021 aggccatgtt gtccggcatt ggatcaagcg caaatgacaa aatgatgtaa gcaaccaggg
    28081 taaacagcgc cagaccgaag aaaatcgaca gcaccaccag gatacgcacc agttttaccg
    28141 gtacatcaaa atagttggca atcccggcgc agacgccgcg gaccatgccc tgctgtggaa
    28201 tacgccataa ttttttattg agattaatgc ccgccattag cgatccctcc agttcggatg
    28261 ttctgcatca agaatagatt ccagcgcctg aatacgttcg cgcatccgtt ttgcttcatc
    28321 agccagttgc gctaatcgct gctgctcact ttgcgacaat tcatcgcgac cagaacgatt
    28381 gctgtaatgc agccataacc agatcggtaa aacaaacagc acaaaaatgg ttaacggaat
    28441 agccagaaat agcgcgctca taagtactcc ttagacgatg agcggcggcg cgttcggacg
    28501 ccgcctggat gttattattg attgtcttgc ttcattttgg cttttaattg tgccagttgt
    28561 tcgctgattg catcatcggc tttcagttcg gcaaactgat cgtccagcga tttttgttta
    28621 ccgaagctgt ggctttctgc ttccgcttcc atctggtcaa ttcgacgttc gaaagattcg
    28681 aaacgagcca ttgcttcatc cagtttgcca ctgtccagct gacgacgcac atcgcgcgac
    28741 gagtttgcgg cctgatggcg taacatcaat gcctgctggc gagcgcgtgt ttcgctcaat
    28801 ttgttttcca gctcaccgat ctctttcttc atgcgtgcca gcgtgtcgtc caccagcgtc
    28861 acttcatgtt ccaggctctt aatcagatcg gtcagtttct gtttttcaat taacgccgca
    28921 cgtgccaaat cctctctctc tttcagcagc gccagttcgg ctttttcctg ccattcaact
    28981 tcacgcgccg acgcttgttc aatacggcga gtcagctgtt tcttttctgc caacgcgcgc
    29041 gccgaagtag aacgtacttc aaccagtgta tcttccatct cctggatcat cagacgaacc
    29101 agtttctgtg gatcttccgc tttctctaac agagcgttga tgttggcatt cacgatgtcg
    29161 gcaaagcgag aaaaaatacc cataatgttg tcctcttgat ttctgcgttc aggattgtcc
    29221 tgctgaactg ttaatacaat ttgcgtgcca attttttatc tttttgattt ataaagatct
    29281 gattgaagaa tcaacagcaa catgccagga tgagttagcg aattacacta acaagtggcg
    29341 aatttcatca tggcagaata caaagataat ttacttggtg aggcgaacag ctttctcgaa
    29401 gtgctggaac aggtttcgca tctcgcaccg ctggacaaac cggtgctcat catcggcgaa
    29461 cgcggcaccg gtaaagagct gattgccagc cgcctgcatt atctctcctc ccgttggcaa
    29521 gggccgttta tttcccttaa ctgcgcggcg ttaaatgaaa atctgctgga ttccgaactg
    29581 tttggtcacg aagcgggggc gtttaccggt gcgcaaaaac gtcatccagg gagatttgaa
    29641 cgtgccgacg gcggtacgct atttcttgat gaactcgcta cggcaccgat gatggtgcag
    29701 gagaaattat tgcgcgtgat tgagtacggt gaactggagc gcgttggcgg cagccagcca
    29761 ttgcaggtga atgtgcggtt ggtatgcgcg acgaatgccg atctcccggc gatggtcaat
    29821 gaaggcactt ttcgcgctga cctgctcgac cgactggctt ttgatgttgt acaactgcca
    29881 ccactgcgcg agcgcgaaag cgacataatg ttgatggcag aacactttgc catccagatg
    29941 tgtcgggaaa tcaagctgcc tctgttcccg gggtttacgg agcgcgccag agaaacattg
    30001 ctgaattatc gttggccggg aaatattcgt gaattgaaaa acgtggtgga acgttcagtg
    30061 tatcgccacg gcaccagcga ttatccgctt gatgacatca ttattgatcc ctttaaacgg
    30121 cgtccgcctg aagacgctat cgccgtttca gaaaccacct cgcttccaac actgccgctg
    30181 gatttacgtg agtttcagat gcagcaggaa aaagagttgc tgcaactcag tttgcaacaa
    30241 gggaaatata accagaagcg cgcggctgaa ttactggggt taacttatca tcagttccgc
    30301 gcgttgttga aaaagcacca gatttagcga agttgagcaa tacaacttgc cggatgcggc
    30361 gtgaatgctt tattcggcat acataatcaa tacgtcgttt gcaggcctga taagcgcagc
    30421 gcatcagaca ttattgcgtt gggctattaa tcgctcagcg catcctgcaa aattttcatt
    30481 gccgcatcga attgcgcatc cgggatggtc agcggataca ggaagcgaat cacgttgccg
    30541 tatgcgccac aggtcagcag gagcagcccc tgcgccagcg cgcgttgctg gattttctgt
    30601 gcaatcgccg ctgacggctc gcccgtttgc ggatcgttaa actctgccgc aatcatcgac
    30661 cccaggccgc gtaccgcagc aatggccgga acgctttctt tggcatcaat caacgtgttt
    30721 ttgagacgct ggcccagttg attcgcgcgt tcgcagagtg attctttgtc gataatattg
    30781 agcaccgcgt gcgcggcagc caccgccagc gggttcccgg cgtaggtgcc acccaacccg
    30841 cccggcgcgg gcgcgtccat aatattcgcg ttaccgacca cgcccgaaag cggcatcccg
    30901 cccgcgaggc ttttcgccat cgtcattaaa tccggcttat cgacgtaatg atccatggca
    30961 aacagcttac cggtacgcgc aaagccgctt tgcacttcat cggcaatcat cacaatgccg
    31021 tgctcgtcgc acaggcggcg aatggcggca accagctctt ttggcgcaac gttgaaaccg
    31081 ccctcgccct gcaccggttc gaaaataatc gccgccacct gcttcgcttc gatgtctgat
    31141 ttaaacaagc gttcgatggc gtcgagggag tcctgtgttg aaatgccgtg taaatctgac
    31201 ggataaggta cgtgatacac cgaaccaggg aacgggccga agccgatttt gtacggagcg
    31261 acctttccag ttaacgccat agtcatatac gtacgaccgt ggaagccgcc gctaaacgca
    31321 atcacgccag ggcgtccggt atgggcgcga gcaattttca ccgcgttttc caccgcttcc
    31381 gcgccggtag tgaagaacgc ggttttggcc tgcccgctca ccggggcaag ggcgttgatt
    31441 ttctccgcca gggtgacgta gctttcatac ggcacaatct gatacgcggt gtgggtaaac
    31501 tgttgcagtt gctgctctac cgccgcgacc agttcagggt ggcggtggcc ggtatttagc
    31561 accgcaatgc ctgcggcgaa atcgatgtac tcgttgccct caacatcctt cagcgtggcg
    31621 ttttcagccg actgggcgaa gaagttacac atcacgccaa ccccgcgtgg ggtagcagaa
    31681 agacgacgct gatggaattc attgttgctc atgtttcaca ccttttgact cttcactgtt
    31741 aatcggaaat taaacgccca gacgatcgcg caggctgtaa taggctgcgc ccatcgcggt
    31801 aaacggtact cgcagcgtgc gcccgccggg gaacgggtaa tgcggcagat tggcgaaggc
    31861 atcgaaacgt tcggcgtcgc cgcgcagcag ttcggcaatc agacgcccgg caagatgggt
    31921 acaggtgacg ccgtggccgc tgtagccctg catgtaatag atgttggtat cgaggcgacc
    31981 gaactgcggc atccgtgaaa gggtcagcag gaagttgccc gtccagcggt agtcgatttt
    32041 gacgcccttc agttgcggga aggttttcag cagtttcggc accacaagac gctcaacgtc
    32101 atccgggtcg cgcgcgccgt agaccacgcc gccgccgtac agcaaacggt tgtcggcggt
    32161 aagacggtag taatcaagca gataattaca gtcttccaca cagtagtttt tcgggatcag
    32221 cgaacgggct aaatcttccg acagccgttc ggtggtgatc acctgcgtgc cgcacggcat
    32281 gctgcgtttc gccagttccg gctctacttt atcgcccaga tacgcattcc cggcgacaat
    32341 cacatacttc gccgtcacct gacctttggc agtgcgcacc accgctggcg tggtgtgctg
    32401 gatctgcgtc acggcagaaa gttcatacac gcgcccgccg ttgaggcgga tggcgtccgc
    32461 ttcgccaatg gcgaggttta gcggatgaat atgcccaccg ctgtgatcca gcagcgcacc
    32521 ggtatagcga tcgctggcta cttcacggcg aatggcgttg gcgtccagca attccagctg
    32581 tttattgccg tagcgttccc agttctcttt ctgctcttca agtgtggcga gctgtttatc
    32641 attcatcgcc acaaacaggc cgccggggcg gtagtcgcag tcaatctgat aacgtttgat
    32701 acgttcgcgg atgatctcac caccttcgaa catcatgctg ccgagcatac gggcggtgtc
    32761 catgccgtag cttttttcga tcacgtcgat gtcgcggctg taggagttca caagctgccc
    32821 gccattgcgc ccgcttgcgc caaagccgat gcgtgaggct tcgagaacca ctacgtcaaa
    32881 gcccgcttcc gccagatgca gcgcggaaga gagcccggta tagccgccgc cgaccacgca
    32941 aacgtcgcag gaaatcgact cattcagcgt gtcgaatggc gcgtatttat tcgcactggc
    33001 ggcgtagtaa ctgctggtat gttcggtcat ttcaggcctc caggcttatc cagatggttt
    33061 tcagttcggt gaatttttca agggcatgca gggatttgtc gcgaccgttg ccgctctgct
    33121 tatagccgcc aaacggcacg gtcatatcgc cgtcgttgta gttattgacg aagacggaac
    33181 cagccttcag gcggcggctc atgcggtgcg cgcgggagag gtcgcgcgtc cataccgccg
    33241 cgccaaggcc gtactggctg tcgttggcaa gctgtagcgc ctggtcttct gatgtgaaac
    33301 gcgtgaccac cagcaccgga ccgaaaatct cttcgcggct taaggacgca ttcgggtcta
    33361 catccacaaa gatggtcggg ccgatggcgg cagccagctc agcgttacga ccatccagca
    33421 gcagttgccc tttgctttcg ccttcccgaa taaagctatg gaccgagtca gcgtgagcgc
    33481 tgtcgattaa ggtgcccatg gtggttgcgg gatcaagtgg atggcccggc tgccagtttt
    33541 gcgcctgctg ttttaacagg gctaagaatt catcggcgat gctctcttcc agtaacaaac
    33601 gcgttccggc gatgcacacc tggccctggt tgtagaagat gcctgcggcg gtggcgcttg
    33661 ccgctttttg cagatccgga caatcggcga aaacgatgtt ggcgcttttg ccgcccgctt
    33721 ccagccagac gcgtttcatg ttgctctcgc ccgcatcttt cagcagctgt ttcccggtac
    33781 gggtcgaacc ggtaaaggca atggcgtcga tatcgttatg acgcgacagc gcctgccccg
    33841 cttcatgacc aaaacctgtc accacgttca aaacaccatc cggcaagcct gcttctttcg
    33901 ccagccccgc gagacgaatc gcactgagcg gtgatttttc agacggtttc aggacaacgc
    33961 tgttccctgc tgccagcgcc gggccaagct tccagcaggt cagcagcagc ggaaagttcc
    34021 acggtacgat ggcggcaatc acgccgaccg gttcacgcac gatcatcgcc agctcatggc
    34081 tactggtggt cgccacttcg ccatacactt tgtcgatcgc ttcggcgtac cagcgaatgg
    34141 cgcgcgccgc gccgggaata tcatcacgca gactgtgacg aatcggtttg ccggtgtcga
    34201 gagtttccag cagtgccagc tcttcggcgt gggcttccat taaatcggcg agtttattca
    34261 gtaccgcttt acgtttagcc ggagaagaga gtgaccagtc gccgcgttca aatacgccgc
    34321 gtgctgcgct caccgcacgg tcgatatcga cgctcttgcc gcgggcaatt ttcgccagcg
    34381 gtgcctgggt gaccggatca acggtttcaa aggtttcatt ttccgccgca gcagtatatt
    34441 caccgttaat aaataagcgg ttttcaatgg cgagacttaa cgctttatcc tgccagtaag
    34501 ccagatgatg aaaattcatt atgactcctg tttcacgtct atcagatata tgcagtttgt
    34561 gaacgtcagg gcgcagagat ccccgggccg catgcccgtt tatttctggc gacattcttt
    34621 atatacactt catccttcaa gccgcctctg ccttggctgc gtttgctccc ccgaatcact
    34681 tacctgagta agctcatcgg gattcgctca cttgccgcct tgatgcaact tgaatgattt
    34741 tgtgtataga ttgccgggcg cattgcgctt acccggctta cagaacaaga agattaaaac
    34801 gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt actgaaactg
    34861 tgcgggatgc cggtattaat ggcataactt tgccccgcga cgagatgata atcctgacca
    34921 ttaatcgtca gaacaatttc accttccagt acagtgccta tttcctcacc ctgatgctta
    34981 attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga
    35041 ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat ctcaattaag
    35101 tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga aaagaattcc
    35161 gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc agggctgact
    35221 ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc ggcggcacga
    35281 cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg cgccagtccc
    35341 tcatcactca tatttgcatt tccttaaact gtagtagtca gagtcgctgt ttttcagcga
    35401 tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc gcgtactcgc
    35461 tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg atgacgctaa
    35521 ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg ctaacgacct
    35581 tcgccccttg tccatgcaga gagtttaccc aaaagttgct acattcaggt aacaacgcag
    35641 acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc tgttccaccg
    35701 gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc ttgcgatgca
    35761 acgaaccacc ggttgccacc accagttcct gtaaaccccg gcagatggcg aaaatgggga
    35821 tgcgcctttc gagtgcggca ttaattaacg ccatgctcag aagatcacgc ccgggatcgg
    35881 cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg ggactaccag
    35941 gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taacgacggt tccgccagcg
    36001 catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg tacttttctt
    36061 gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca ccgataaccg
    36121 gattgttcat tatattttcc atgctcaatc tcacaaagtg gactaaatta tcgccattac
    36181 tgcgctttaa atgaccttta tgttcaatat tttttcaata tagcagtggg ttttcatttt
    36241 tgcaaactca atttaacatt tgacaaacat ttagtttgca tacagattcg aatggtggtc
    36301 attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga atcatggaaa
    36361 ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg ggtagcgcct
    36421 ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat gttgatgttt
    36481 tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga
    36541 agttggagaa agggtgttat ttcccggcct cggtgtttgc aatggatatt ctgggcaacg
    36601 tagtagaaga agcgggtctg ggtcaggaaa tgggcgagcc ggatcgtact tgtgttcctg
    36661 ttctcggttc cttaacccct tctgccgccg atccagagtt tatcggtcag atgctcctga
    36721 ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt cttaaccgcc
    36781 tgtggcagca gctgcgccag cgcggattgt tcccggtcgt agcggtagag ctggagttct
    36841 atttactgga tcgccagcgc gacgctgaag ggtatctgca accgccctgc gcgcccggca
    36901 ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac cacttcgccg
    36961 acgtgctcaa tgatattgat gaactggcgc agttacagtt aattcccgcc gacggtgcgg
    37021 tcgccgaagc ctcgccgggt cagtttgaaa tcaaccttta ccataccgat aacgtgctgg
    37081 aagcctgcga tgatgcactg gcgctaaaac gcctggtgcg tctgatggca gaaaagcata
    37141 agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc gggatgcata
    37201 tccatatcag tatgcaaaat aatcgtggcg agaatgtgct ttctgacaca gaaggcgaag
    37261 attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg tcgtcgatgg
    37321 cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccgggaatg tatgtgccga
    37381 cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccg tgcggcgacc
    37441 gtcataatca ccgcgtggaa tatcgcgtgg cgggtgccga tgccaaccca tatctggtga
    37501 tggcagcgat ttttgccggt attttgcatg gccttgataa cgagctgccg ttgcaggaag
    37561 aagtcgaagg caacgggctg gaacaggaag gcttaccctt cccgattcgc cagagcgatg
    37621 ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa cggttttgcc
    37681 atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc atcacagaaa
    37741 cagaaattga gtggatgttg aaaaacgcgt aattccccat gcggggttgt ttcatcggcc
    37801 tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat gtcgcgttat
    37861 ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacgagg aaatgagatg
    37921 atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc tcctgctact
    37981 gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag gttgacacga
    38041 ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac cactgaatat
    38101 tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc aggtggtaat
    38161 gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta ttgtctccgg
    38221 cattagcgac ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg tgctgtttac
    38281 cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg cctataccta
    38341 cgcgcaaaag tcgattaatc cgcacgtcgg atttatggtc ggctggtcat cactgctgga
    38401 ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa atctatctct ccgccctctt
    38461 cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa ccgccgcgaa
    38521 tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg tgcaaatctc
    38581 catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag aaggcgttgg
    38641 caccgtctgg tcgttacagc cgtttatcag cgagaacgcg catctgattc cgattattac
    38701 cggggcgacg atcgtctgct tctctttcct tggtttcgat gcagtgacca cactttcgga
    38761 agagacacca gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg cggtctatgg
    38821 tggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttcttccccg atatcagccg
    38881 ctttaaagac ccggacgccg cactgcctga aattgcactc tacgtcggcg gtaagctgtt
    38941 ccagtcgatt ttcctttgca ccacgtttgt gaacacgtta gcgtctggcc tggcctcaca
    39001 tgccagcgtg tcacgtctgc tgtatgtgat ggggcgcgac aatgtgtttc cggagcgcgt
    39061 gtttggctat gtgcacccaa aatggcggac tccggcactc aacgtcatta tggtcgggat
    39121 tgtcgcgttg tcggcgctgt tcttcgattt agttaccgcc acagcgttaa ttaacttcgg
    39181 tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct ggcggcgtaa
    39241 aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc tggttggcgc
    39301 gctgacggtg ggtgtgctgt gggttaacct cgagtcaacg tcactgacac tcggtctggt
    39361 atgggcttcg ttaggcggcg catatttatg gtatctgatc cgccgctatc gcaaagtgcc
    39421 gttgtatgat ggtgacagaa cgccggtgag tgaaacgtaa tagtcggatg cgcggcgcat
    39481 tatcctgcct gagggtaacg cgccgcgcca ttaactataa aaagatgtat gacccggatc
    39541 acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa ataatgaacc
    39601 acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa tgcgcagagc
    39661 ctgtcgcaga cgctgaacgc acaccgctgg cccactactt ccagttactg ctcacccgtt
    39721 tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct gaagcgggga
    39781 ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt aatgagtaat
    39841 tatttaattg cagacgatcg gtcagtatcg ttgtccggat aaagcgttca ctctgcatcc
    39901 ggttaaaatg acacgcattg cagcgtgctt taatgggttt ctcacgatcg taccgctctg
    39961 ctcctcgcta agttgttctg tgagttatag ttatagagaa atcacgggta cgaatgttga
    40021 acagatggcg acgccatctg ttcaacgacc aaccacacgg ccctcattac gacccctgaa
    40081 cacaaacggt aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc
    40141 ccttaatatt atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg
    40201 tcaggcaatc gccgcgcctg aatctccccc gcatgctgat atccgcgaca gcggttttgt
    40261 ctattgcgtc agcgggcaag tcaacacctt taacccatcc aaagcgagca gtgggttaat
    40321 tgtcgatacc cttgccgccc agttttatga tcgactgctg gatgtcgatc cctataccta
    40381 tcgcctgatg ccggaacttg ccgaaagctg ggaagtactc gacaacggcg cgacctatcg
    40441 cttccacctg cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa
    40501 aatgaatgcc gacgatgtgg tgtttacctt ccagcgaatt tttgaccgca acaacccgtg
    40561 gcataacgtc aacggcagca acttccccta cttcgacagc ctgcaatttg ccgataacgt
    40621 gaaaagcgtc cgcaaactgg ataatcatac cgttgagttc cgactcgccc agccggatgc
    40681 ttcttttttg tggcacctcg caacccatta tgcttcggtc atgtcggcag aatatgcccg
    40741 gaagttagag aaagaagatc gccaggagca actcgaccgt caaccggtcg gcaccggtcc
    40801 gtatcagttg tcggaatacc gcgccgggca atttattcgc ctacaacgtc atgatgactt
    40861 ctggcgcggt aaaccgttaa tgccgcaggt agtggtggat ttaggctccg gcggcaccgg
    40921 acgtctgtcg aaactcctga ccggggaatg cgacgttctg gcctggcctg ctgccagcca
    40981 gctatccatt ttgcgtgacg acccgcgctt gcgtttaacg ctgcgtcctg ggatgaacgt
    41041 cgcctatctg gcatttaaca ccgccaaacc gccgctaaat aatcccgctg tccgccatgc
    41101 gctggcactg gcgattaata accagcgcct gatgcaatcc atctattatg gtacggctga
    41161 aacggcggcc tctattttac cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac
    41221 tgaatacaat ccggcgaaat cgcgcgaaca gttgaagtcg ttggggctgg aaaatttaac
    41281 gctgaaactg tgggtgccca cacgttcgca ggcgtggaac cccagtccac tgaaaactgc
    41341 cgaactgatt caggcggata tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga
    41401 aggtcgcttt caggaggcgc ggttgatgga tatgagccat gatctgacgt tatccggttg
    41461 ggcgacggac agtaacgacc cggacagttt cttccgtccg ttactgagct gcgcggcaat
    41521 tcattcacag accaacctcg cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa
    41581 ggcgctctcc tcgcagcagc tggcagcgcg tattgaagcc tatgacgaag cgcagagtat
    41641 tctggcgcag gaattaccta ttctgccgct ggcgtcgtca ttgcgtttgc aggcctatcg
    41701 gtacgatatc aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta
    41761 tcgcgagaaa caggatgagg tgaaaaaacc atgattatct tcaccttacg ccgcattttg
    41821 ttattgattg tcaccttgtt cctgctgacc ttcgttggct tcagtttgag ctatttcact
    41881 ccacacgcgc cgttacaagg cgcgtcactg tggaatgcct gggtgttctg gtttaacggc
    41941 ctgatccact gggattttgg tgtttccagc attaacggtc aaccgattgc cgaacagtta
    42001 aaagaggtct tccctgcgac gatggaactg tgcatcctcg ccttcggttt tgcgctgatt
    42061 gttggcatcc ccgtggggat gattgccggg attacgcgcc ataagtggca ggataacttg
    42121 atcaacgcca tcgccttgct gggcttttca atcccggtgt tctggctggc gcttctgttg
    42181 acgctgtttt gttcactcac gctaggttgg ttgcccgttt cagggcgttt cgatctgctg
    42241 tacgaagtga aaccgattac cgggtttgcg ttgattgatg cctggctttc ggactcacca
    42301 tggcgggatg aaatgatcat gagcgcaatc cgccatatga tattgcccgt gatcaccctg
    42361 tcggttgcgc caacaactga agtgatccga ctgatgcgta tcagcactat cgaagtgtat
    42421 gaccagaact atgttaaagc ggcggctacc cgcggtttgt cgcgctttac tattttgcgt
    42481 cgccacgttc tgcataacgc gttaccgccg gttattcctc gcctgggctt acagttttcc
    42541 accatgctga cgctggcaat gatcaccgaa atggttttta gctggccggg tttagggcgc
    42601 tggttaatta acgccatccg ccagcaggat tacgcagcca tttccgccgg agtgatggtg
    42661 tgtggctcac tggtcattat tgttaacgtg atttctgata ttttgggtgc catggctaac
    42721 cctctgaaac ataaggaatg gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc
    42781 gggcacgctg cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct
    42841 gtacggctgc gcggggctgg ctgtactgtg tatttttggc ggctggtttg cgccttacgg
    42901 catcgaccag caatttctcg gttaccaatt attgccgccg tcatggtcgc gctatggcga
    42961 agtttctttc ttcctgggga ctgacgacct cggacgcgat gttttaagcc gtttactgag
    43021 cggagctgcg ccaaccgtgg gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg
    43081 cctggtactg ggcaccttcg ccggggcgac gcacggccta cgctcagcgg tgcttaacca
    43141 tattctggat accttactgg cgatcccttc gctgctgctg gcaattatcg ttgtagcgtt
    43201 tgccggaccg agtttgtccc acgctatgtt tgctgtctgg ctggcattgc tgccgcgtat
    43261 ggtacgttcg atttacagca tggtgcatga cgaactggaa aaagagtacg ttatcgccgc
    43321 ccgtctggat ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc caaacatcac
    43381 cgccgggctg gtcaccgaga tcacccgagc actgtcgatg gcaattctcg atatcgccgc
    43441 gctgggcttt ctcgatctcg gcgcacaact cccctcgcct gaatggggag cgatgctcgg
    43501 tgatgcgctg gaactgattt atgtcgcccc gtggactgtc atgctgccag gtgcggcaat
    43561 tatgattagc gtgttgttgg ttaacctcct tggcgatggc gttcgtcgtg cgattattgc
    43621 gggggtggaa taatgccatt actcgatatt cgtaacctga ccattgaatt taaaaccggt
    43681 gatgagtggg ttaaagccgt cgaccgggta agtatgacct taaccgaagg tgaaatccgc
    43741 ggtcttgttg gtgaatccgg ttccggcaag agtttgattg cgaaagcgat ttgtggggtg
    43801 aataaagata actggcgtgt tactgctgac cgtatgcgtt ttgatgatat cgatttgctg
    43861 cgtctctccg cacgcgaacg gcgcaaactg gttggccata acgtgtcgat gattttccag
    43921 gaaccgcagt cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc
    43981 ccagcctgga cctataaagg ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc
    44041 atcgaactac tgcaccgcgt ggggattaaa gatcacaaag atgctatgcg cagttttccc
    44101 tatgagttaa ccgaaggtga atgtcaaaaa gtgatgatag ccattgcact ggcgaatcaa
    44161 ccgcgtctgc taattgctga cgaaccgacc aactcaatgg agccaacaac ccaggcgcaa
    44221 atctttcgcc tgttgacgcg tctcaaccaa aacagcaata ccactatttt gcttatcagc
    44281 catgacttac aaatgcttag ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa
    44341 acggtggaaa ccgcgccaag taaggagttg gtgacaatgc cacatcatcc ttatacccag
    44401 gcgctgatcc gggcgatacc agacttcggt agcgcgatgc cgcataaaag tcgcctcaat
    44461 acgctgcccg gcgctatccc actgctggaa cagttaccga ttggttgtcg tctggggccg
    44521 cgttgcccgt acgcacaacg agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat
    44581 catctctatg cctgtcattt cccgctgaac atggagaaag agtgagatga tcgaaacgct
    44641 gcttgaagtg cgtaatttaa gtaaaacgtt ccgctaccgg accggttggt ttcgtcgtca
    44701 gaccgtagaa gcggtaaaac ccttgagctt tacgctacgt gaaggccaga cactggcgat
    44761 tattggcgag aatggttcgg gtaaatccac gctggcaaaa atgctggcgg gaatgataga
    44821 acctaccagc ggcgagttat tgattgacga tcatccactg cattttggcg attattcctt
    44881 ccgcagtcag cgtattcgca tgatttttca ggatccttcg acctcgttga atccccgtca
    44941 acgtatctcg caaattctgg attttccact gcgcctgaac accaacctgg aaccggaaca
    45001 gcgtcgcaaa cagattattg aaacgatgcg tatggttggg ctgctaccgg atcacgtcag
    45061 ttactatccg catatgttgg cccccgggca aaaacagcgt ctgggtctgg cgcgcgcgct
    45121 gatattgcgc ccaaaagtca ttattgccga tgaagccctc gcttcactgg atatgtcgat
    45181 gcgttcgcag ttgattaatc tgatgctgga attacaggaa aaacagggca tttcgtatat
    45241 ttatgttacc cagcatatcg gaatgatgaa gcacattagc gaccaggtgc tggtgatgca
    45301 tcagggcgag gttgtcgagc gaggcagcac cgcggatgtg ctggcatcgc cgctgcatga
    45361 actcaccaaa cggctgattg ccgggcactt tggcgaagca ttgacagcgg atgcgtggcg
    45421 taaagatcgc taattttatg aatcctcctt ccagattatc cacctcattc tgacaagaga
    45481 ttaaatcttg tcagaatgag gtgatggata aaataaaatc aaatgcgcgt gatttaagac
    45541 gcaatctcac tttgcaggaa cgaaagctct ggcgatatct tcgcagccga cgttttggtg
    45601 atttcaaatt tcgccgtcaa catccggtgg ggagctacat tctcgatttt gcttgctgct
    45661 cggcgcgcgt agtcgttgag ctggatggtg ggcagcatga tttggcagtt gcctatgata
    45721 ccaggcgcac tagctggctt gagtcgcaga gctggaccgt gctgcgtttc tggcataacg
    45781 agattgattg taatgaggag gcggtgctgg agattattct gcaggaactg aaccgccggt
    45841 caccctcacc ctgaaagggc gagggggccg accgagccga atagctgttg tggtgaaaac
    45901 atggagacag tgctggagaa tattcggcaa ggtctgaacc gtcccagcca tcgccctgaa
    45961 agggttaggg ggctgtataa gcctgtttgt tgctggggtg acaatatttg cacaatatgg
    46021 tcccctcgcc cctctgggga gaggattagg gtgtggggaa aagcgcccac cctgcccgca
    46081 ccctgctccg gtcggacctg gcagctatag ccactcacag ccaggttgat tataataacc
    46141 gtttatctgt tcgtactgtt tactaaaacg acgaatcgcc tgattttcag gcacaacaag
    46201 catcaacaat aaggattaaa gctatgggtt ttctttccgg taagcgcatt ctggtaaccg
    46261 gtgttgccag caaactatcc atcgcctacg gtatcgctca ggcgatgcac cgcgaaggag
    46321 ctgaactggc attcacctac cagaacgaca aactgaaagg ccgcgtagaa gaatttgccg
    46381 ctcaattggg ttctgacatc gttctgcagt gcgatgttgc agaagatgcc agcatcgaca
    46441 ccatgttcgc tgaactgggg aaagtttggc cgaaatttga cggtttcgta cactctattg
    46501 gttttgcacc tggcgatcag ctggatggtg actatgttaa cgccgttacc cgtgaaggct
    46561 tcaaaattgc ccacgacatc agctcctaca gcttcgttgc aatggcaaaa gcttgccgct
    46621 ccatgctgaa tccgggttct gccctgctga ccctttccta ccttggcgct gagcgcgcta
    46681 tcccgaacta caacgttatg ggtctggcaa aagcgtctct ggaagcgaac gtgcgctata
    46741 tggcgaacgc gatgggtccg gaaggtgtgc gtgttaacgc catctctgct ggtccgatcc
    46801 gtactctggc ggcctccggt atcaaagact tccgcaaaat gctggctcat tgcgaagccg
    46861 ttaccccgat tcgccgtacc gttactattg aagatgtggg taactccgcg gcattcctgt
    46921 gctccgatct ctctgccggt atctccggtg aagtgatcca cgttgacggc ggtttcagca
    46981 tcgctgcaat gaacgaactc gaattgaaat aatcattctg ttggtaaata ttgggcggcg
    47041 ttctgccgcc cgttatctct gttatacctt tctgatattt gttatcgccg atccgtcttt
    47101 ctccccttcc cgccttgcgt caggataacg atttccttta cgaccaagga gcgcccatgg
    47161 aacaacgcca catcaccggc aaaagccact ggtatcatga aacgcaatcc agtactgcgg
    47221 agtatgacgt tctgcctctg gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg
    47281 acgtgatcct tgatgaagaa acgctggctc ccttcctttc atggctggtc cctgcgcgcg
    47341 ttcttgcagt ggaattgttc cctgaccagc ttaccgtgac ccgttcacag accttcaccg
    47401 cttatgaacg cttgtctacg gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat
    47461 gtaactacta ttcggcgcga cttacgccgc tccccgggcc tgattcctcc agggaaagca
    47521 atcataggtt ggcacaaatc acgcaatatg cccgccaact ggctagctcg ccttctatta
    47581 tcgacaaccg atcgcgccag catctgaatg acgtcggtct tactgccggg gactgtgtga
    47641 tcattaacca aatcattggt tttattggct ttcaggcgcg gaccattgcg acatttcagg
    47701 cttatctcgg gcacccggta cgctggttac ccggtctgga aatacaaaac tacgccgacg
    47761 cgtcactgtt tgctgatgaa tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac
    47821 ctgaagagta cacaaaaagt tcaacagcag aactttgcca actggccgaa acactctctc
    47881 tccaccctat ttcactttcc cttctcgaaa ggttgttaaa cagtacacgg gttaatacac
    47941 agccggataa taagcttgcg gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt
    48001 ttgccgcctg tatggattca tcaaatgaat ataaaaaaat cagccccctt ctgcgcaagg
    48061 gcgaaaatga aattaaccaa tgggctgacc gtcattctgt tgagcacgct accgttcagg
    48121 cgatacaatg gctgacccga gcacccgatc gctttagcgc cgcccagttc agccctttac
    48181 tcgaacacga aaaatcatca acgcagatta ttaatctgct ggtatggagc gggctgtgtg
    48241 gctggataaa tcgcttaaaa atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac
    48301 agattcgcgt aaaactgtca gccgctctaa tggccaccaa aatagacaat tatgtttcag
    48361 gacaacccgc tgctagcgca gcttaaacag caactgcatt cccagacgcc acgcgctgaa
    48421 ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt
    48481 tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg
    48541 atccacagtg aaaaagaacg tgaatccgca gagccagaag aactggttga accgttcctg
    48601 actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat
    48661 ccactcttaa aagacgccat tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa
    48721 gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc
    48781 tatgctgaac tgacacaata catcactttt ggtgacgacc actttgtacc gtggtgggtt
    48841 acccttgcgc gccataatct ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc
    48901 gatgaaggtc tggttcgtga agatctgacc gcgctggatt ttgtcaccat cgacagtgcc
    48961 agcacagaag atatggatga cgcccttttc gctaaggcgt tgccggatga caaacttcag
    49021 ctgattgtgg cgattgccga tccaaccgcg tggattgctg aaggcagcaa gctggacaaa
    49081 gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct tcaacatccc tatgctgcct
    49141 cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg aagtccgccc ggtactggca
    49201 tgccgcatga cgctctccgc tgatggcacc attgaagata atatcgaatt ctttgccgcc
    49261 accatcgaat ccaaagcaaa gctggtgtat gaccaggttt ctgactggct ggaaaatacc
    49321 ggtgactggc agcctgaaag tgaagcaatt gccgaacaag tccgtttgtt agcgcaaatt
    49381 tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg tgtttaaaga tcgcccggat
    49441 taccgcttta ttctcggtga aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc
    49501 attgccaacc gtatcgtcga agaagcgatg attgccgcta acatttgtgc ggcccgcgta
    49561 ctgcgcgata agctcggttt tggcatctat aacgtgcata tgggctttga tccggcgaat
    49621 gccgacgcgc tggcagcgtt gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg
    49681 ctcacgctgg acggtttctg caaactgcgt cgtgaactgg atgcgcaacc aactggtttc
    49741 ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa ttagcactga acccggtcct
    49801 cactttggcc tcggtctgga agcatacgcc acctggactt cgccgatccg taaatatggc
    49861 gacatgatca accaccgtct gctgaaagcg gttatcaaag gcgaaactgc gacgcgccca
    49921 caggatgaaa tcactgtcca aatggccgag cgtcgccgtc tcaaccggat ggcagaacgt
    49981 gatgttggcg actggttata cgcacgcttc ctgaaagaca aagccgggac cgacacccgt
    50041 ttcgcggcgg aaattgtcga tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac
    50101 ggcgctatcg cctttattcc ggcacctttc ttacacgctg tgcgcgatga actggtttgc
    50161 agccaggaaa acggcactgt acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt
    50221 attgacgtca ccattgccga agtccgcatg gaaacccgca gcattattgc gcgcccggtc
    50281 gcgtaatctc ctttcacggc ccattcctca tggatgggcc gtttatttcc ccgctctacc
    50341 ttcgttatat ccaggcagat tttttaactg actttcgttt gaaaactggc gtttttccaa
    50401 aactggatta caaataaata tataaaaaaa acaatgcgtt tagccgccgt taatttcatg
    50461 taagtcatgt tgttcctttt atttaaccgc ggactccgca gttaaccgga ggatatgcat
    50521 catgaaaacc gttagggagt ccacaacgtt gtacaacttt ctcggatcgc acaatccata
    50581 ctggcggttg acggaaagca gcgatgtttt gcgcttttct accaccgaaa ccacagaacc
    50641 tgatcgtact ttgcagttat ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat
    50701 cacctccagc ctgatgatga gtctgaccgt cgatgagagc gatctttctg tgcatctggt
    50761 aggacgaaaa atcaataaac gggaatgggc tggcaacgcg tctgcatggc atgacacacc
    50821 ggcagttgct cgtgatttat cacacgggct ttcctttgct gagcaggtag tttctgaagc
    50881 acattccgca atagtgattc tcgacagccg ggggaatatc caacgcttca atcggttatg
    50941 tgaagattac actgggttga aagaacacga cgtcattggc caaagcgtgt ttaaactgtt
    51001 tatgagccgt cgtgaagctg cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg
    51061 caatgcatat gaagtcgaac tgtggatacc aacacgtaaa ggccagcggc tgtttctgtt
    51121 tcgcaataaa tttgtccaca gcggcagtgg caaaaacgag atttttttaa tctgttccgg
    51181 caccgacatt accgaagagc gccgcgctca ggagcgactg cgtattctgg caaataccga
    51241 cagtatcacc ggactgccga atcgtaacgc aatgcaggat ttaatcgatc acgctattaa
    51301 tcatgcagat aacaataaag ttggggttgt gtatcttgat ttggataatt tcaaaaaggt
    51361 caacgacgcc tatgggcatt tgtttggtga ccagttatta cgcgacgtgt cattggctat
    51421 tttaagctgt ctcgaacatg accaggtgtt ggcgcgtcca ggtggcgatg agtttctggt
    51481 actggcatcc aacacctcac aaagcgcgct ggaagcaatg gcatcacgaa ttttgacccg
    51541 cttacggctc ccctttcgca ttggtttaat tgaagtttat accagctgtt cagtaggtat
    51601 tgcactctct cccgaacatg gttcagacag cacggctatt attcgtcacg ccgacacagc
    51661 aatgtacaca gcgaaggaag gcggacgagg acaattttgc gtttttaccc cagaaatgaa
    51721 tcaacgggta tttgaatatc tctggctgga taccaacttg cgtaaagcac tggaaaacga
    51781 tcagttggtt attcactatc aaccgaaaat cacctggcgt ggcgaagtgc gcagtctgga
    51841 agcactagta cgttggcagt cacctgaacg tgggttgatt ccaccgttgg acttcatttc
    51901 ctacgccgaa gagtcagggc taattgtgcc tttaggccgt tgggtgattc tcgatgtcgt
    51961 acgccaggtg gcgaagtggc gggataaagg tattaacctg cgagtggcgg taaatatttc
    52021 tgcacgtcag ctcgccgatc aaaccatttt caccgccctg aaacaggttc tccaggaact
    52081 caattttgaa tactgcccta tagatgttga actgacagag agttgtctga ttgagaatga
    52141 tgaactggca ctgtctgtta ttcaacaatt tagccaacta ggtgcgcaag tgcatctgga
    52201 tgattttggt accggctact cttcactttc gcaactggcg cgctttccga tcgatgccat
    52261 caaacttgac caggtttttg ttcgagatat tcacaaacaa cctgtctcgc agtcactggt
    52321 ccgggcgatc gtcgctgtgg cccaggcatt gaatcttcag gtgatcgccg aaggtgtaga
    52381 gagtgcaaag gaagatgctt ttttaaccaa gaacgggatc aatgagcggc aaggattttt
    52441 gtttgccaaa ccgatgcccg ccgtcgcctt cgaacgctgg tataaacgct atctgaagcg
    52501 cgcataagta gatgaatata tatacttagc gctatatccc gtcattatat atcgtattcc
    52561 tggatgataa ttagccgttg ctgttaaaca cttctctttt agcggcaatt tttgcattat
    52621 tcattttcca cttttgctat aaggatgtcc ttgatgtctg aatttgatgc ccagcgcgtt
    52681 gccgaacgta ttgatattgt gttagatatc cttgtggcag gcgattacca ctctgcgatc
    52741 cataatctcg agatacttaa agcagaactc ttgcgccagg ttgctgaatc tacgccagac
    52801 atcccgaagg caccgtggga gatttaacaa tcgtgccttc aggttttcct
//
LOCUS       DAZFRN010000015        51839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFRN010000015 DAZFRN010000000
VERSION     DAZFRN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.015"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            29..2419
                     /gene="gcd"
                     /locus_tag="ACHKUB_000851"
     CDS             29..2419
                     /gene="gcd"
                     /locus_tag="ACHKUB_000851"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO6653908.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2466..4016)
                     /gene="cueO"
                     /locus_tag="ACHKUB_000852"
     CDS             complement(2466..4016)
                     /gene="cueO"
                     /locus_tag="ACHKUB_000852"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO6653909.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKIEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4182..4529
                     /gene="yacC"
                     /locus_tag="ACHKUB_000853"
     CDS             4182..4529
                     /gene="yacC"
                     /locus_tag="ACHKUB_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO6653910.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4635..5501
                     /gene="speE"
                     /locus_tag="ACHKUB_000854"
     CDS             4635..5501
                     /gene="speE"
                     /locus_tag="ACHKUB_000854"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO6653911.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5517..6311
                     /gene="speD"
                     /locus_tag="ACHKUB_000855"
     CDS             5517..6311
                     /gene="speD"
                     /locus_tag="ACHKUB_000855"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO6653912.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6349..6711)
                     /gene="yacL"
                     /locus_tag="ACHKUB_000856"
     CDS             complement(6349..6711)
                     /gene="yacL"
                     /locus_tag="ACHKUB_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO6653913.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(6886..9483)
                     /gene="acnB"
                     /locus_tag="ACHKUB_000857"
     CDS             complement(6886..9483)
                     /gene="acnB"
                     /locus_tag="ACHKUB_000857"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO6653914.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9838..11691
                     /gene="yacH"
                     /locus_tag="ACHKUB_000858"
     CDS             9838..11691
                     /gene="yacH"
                     /locus_tag="ACHKUB_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO6653915.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(11762..13186)
                     /gene="lpdA"
                     /locus_tag="ACHKUB_000859"
     CDS             complement(11762..13186)
                     /gene="lpdA"
                     /locus_tag="ACHKUB_000859"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO6653916.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13511..15403)
                     /gene="aceF"
                     /locus_tag="ACHKUB_000860"
     CDS             complement(13511..15403)
                     /gene="aceF"
                     /locus_tag="ACHKUB_000860"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO6653917.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKETAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15418..18081)
                     /gene="aceE"
                     /locus_tag="ACHKUB_000861"
     CDS             complement(15418..18081)
                     /gene="aceE"
                     /locus_tag="ACHKUB_000861"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO6653918.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18242..19006)
                     /gene="pdhR"
                     /locus_tag="ACHKUB_000862"
     CDS             complement(18242..19006)
                     /gene="pdhR"
                     /locus_tag="ACHKUB_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO6653919.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            19547..20920
                     /gene="aroP"
                     /locus_tag="ACHKUB_000863"
     CDS             19547..20920
                     /gene="aroP"
                     /locus_tag="ACHKUB_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO6653920.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(20963..21817)
                     /gene="ampE"
                     /locus_tag="ACHKUB_000864"
     CDS             complement(20963..21817)
                     /gene="ampE"
                     /locus_tag="ACHKUB_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO6653921.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(21814..22365)
                     /gene="ampD"
                     /locus_tag="ACHKUB_000865"
     CDS             complement(21814..22365)
                     /gene="ampD"
                     /locus_tag="ACHKUB_000865"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO6653922.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            22453..23346
                     /gene="nadC"
                     /locus_tag="ACHKUB_000866"
     CDS             22453..23346
                     /gene="nadC"
                     /locus_tag="ACHKUB_000866"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO6653923.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            23549..23989
                     /gene="ppdD"
                     /locus_tag="ACHKUB_000867"
     CDS             23549..23989
                     /gene="ppdD"
                     /locus_tag="ACHKUB_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO6653924.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            23999..25384
                     /gene="gspE"
                     /locus_tag="ACHKUB_000868"
     CDS             23999..25384
                     /gene="gspE"
                     /locus_tag="ACHKUB_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO6653925.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            25374..26576
                     /gene="hofC"
                     /locus_tag="ACHKUB_000869"
     CDS             25374..26576
                     /gene="hofC"
                     /locus_tag="ACHKUB_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO6653926.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(26611..27654)
                     /gene="guaC"
                     /locus_tag="ACHKUB_000870"
     CDS             complement(26611..27654)
                     /gene="guaC"
                     /locus_tag="ACHKUB_000870"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO6653927.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(27810..27854)
                     /locus_tag="ACHKUB_000871"
     CDS             complement(27810..27854)
                     /locus_tag="ACHKUB_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6653928.1"
                     /translation="MIIKTLKMSARKRN"
     gene            27879..28499
                     /gene="coaE"
                     /locus_tag="ACHKUB_000872"
     CDS             27879..28499
                     /gene="coaE"
                     /locus_tag="ACHKUB_000872"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO6653929.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            28499..29242
                     /gene="zapD"
                     /locus_tag="ACHKUB_000873"
     CDS             28499..29242
                     /gene="zapD"
                     /locus_tag="ACHKUB_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO6653930.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            29252..29449
                     /gene="yacG"
                     /locus_tag="ACHKUB_000874"
     CDS             29252..29449
                     /gene="yacG"
                     /locus_tag="ACHKUB_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO6653931.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(29549..29938)
                     /gene="mutT"
                     /locus_tag="ACHKUB_000875"
     CDS             complement(29549..29938)
                     /gene="mutT"
                     /locus_tag="ACHKUB_000875"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO6653932.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(29998..32703)
                     /gene="secA"
                     /locus_tag="ACHKUB_000876"
     CDS             complement(29998..32703)
                     /gene="secA"
                     /locus_tag="ACHKUB_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO6653933.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(32765..33277)
                     /gene="secM"
                     /locus_tag="ACHKUB_000877"
     CDS             complement(32765..33277)
                     /gene="secM"
                     /locus_tag="ACHKUB_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO6653934.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(33508..34425)
                     /gene="lpxC"
                     /locus_tag="ACHKUB_000878"
     CDS             complement(33508..34425)
                     /gene="lpxC"
                     /locus_tag="ACHKUB_000878"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO6653935.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPVNTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(34526..35677)
                     /gene="ftsZ"
                     /locus_tag="ACHKUB_000879"
     CDS             complement(34526..35677)
                     /gene="ftsZ"
                     /locus_tag="ACHKUB_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6653936.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(35738..37000)
                     /gene="ftsA"
                     /locus_tag="ACHKUB_000880"
     CDS             complement(35738..37000)
                     /gene="ftsA"
                     /locus_tag="ACHKUB_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO6653937.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(36997..37827)
                     /gene="ftsQ"
                     /locus_tag="ACHKUB_000881"
     CDS             complement(36997..37827)
                     /gene="ftsQ"
                     /locus_tag="ACHKUB_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO6653938.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(37829..38749)
                     /gene="ddlB"
                     /locus_tag="ACHKUB_000882"
     CDS             complement(37829..38749)
                     /gene="ddlB"
                     /locus_tag="ACHKUB_000882"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6653939.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(38742..40217)
                     /gene="murC"
                     /locus_tag="ACHKUB_000883"
     CDS             complement(38742..40217)
                     /gene="murC"
                     /locus_tag="ACHKUB_000883"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO6653940.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(40271..41338)
                     /gene="murG"
                     /locus_tag="ACHKUB_000884"
     CDS             complement(40271..41338)
                     /gene="murG"
                     /locus_tag="ACHKUB_000884"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO6653941.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(41335..42579)
                     /gene="ftsW"
                     /locus_tag="ACHKUB_000885"
     CDS             complement(41335..42579)
                     /gene="ftsW"
                     /locus_tag="ACHKUB_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO6653942.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPTGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(42579..43895)
                     /gene="murD"
                     /locus_tag="ACHKUB_000886"
     CDS             complement(42579..43895)
                     /gene="murD"
                     /locus_tag="ACHKUB_000886"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO6653943.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(43898..44980)
                     /gene="mraY"
                     /locus_tag="ACHKUB_000887"
     CDS             complement(43898..44980)
                     /gene="mraY"
                     /locus_tag="ACHKUB_000887"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO6653944.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(44974..46332)
                     /gene="murF"
                     /locus_tag="ACHKUB_000888"
     CDS             complement(44974..46332)
                     /gene="murF"
                     /locus_tag="ACHKUB_000888"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO6653945.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(46329..47816)
                     /gene="murE"
                     /locus_tag="ACHKUB_000889"
     CDS             complement(46329..47816)
                     /gene="murE"
                     /locus_tag="ACHKUB_000889"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO6653946.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(47803..49569)
                     /gene="ftsI"
                     /locus_tag="ACHKUB_000890"
     CDS             complement(47803..49569)
                     /gene="ftsI"
                     /locus_tag="ACHKUB_000890"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO6653947.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(49585..49950)
                     /gene="ftsL"
                     /locus_tag="ACHKUB_000891"
     CDS             complement(49585..49950)
                     /gene="ftsL"
                     /locus_tag="ACHKUB_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO6653948.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(49947..50888)
                     /gene="rsmH"
                     /locus_tag="ACHKUB_000892"
     CDS             complement(49947..50888)
                     /gene="rsmH"
                     /locus_tag="ACHKUB_000892"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO6653949.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(50890..51348)
                     /gene="mraZ"
                     /locus_tag="ACHKUB_000893"
     CDS             complement(50890..51348)
                     /gene="mraZ"
                     /locus_tag="ACHKUB_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO6653950.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        12157 a        14507 c        12872 g        12303 t
ORIGIN      
        1 tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct cgcgacgatt
       61 actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac tcattggcgg
      121 aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggtc ttgtgatgct
      181 cggcgtcgcc tggatgctgt ggcgcagtaa acgtgccgcg ctttggctgt acgcagccct
      241 gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct gggcgctgac
      301 tccgcgcagc gacattctgg tcttcttcgg catctggctg atcctgccgt ttgtctggcg
      361 tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtggcac tgctgattag
      421 cggtggtatc ctgacctggg ccggatttaa cgatccgcag gaaatcaacg gcaccttaag
      481 cgccaatgcc acacctgcgg aagctatctc acccgttgcc gatcaggact ggcctgccta
      541 tggtcgcaat caggaaggtc aacgcttttc acctctgaaa caaattaacg ccgataacgt
      601 ccataatctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc cgaacgatcc
      661 gggcgaaatc accaatgaag tgacgccgat taaagtgggc gacacccttt acctgtgtac
      721 cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc aaagagaaat ggcattacga
      781 tcctgagctg aaaactaacg agtctttcca gcacgtaacc tgccgtggcg tctcttatca
      841 tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc gtcgtatcat
      901 tcttccggtc aatgatggtc gactgattgc gattaacgct gaaaacggca aactgtgcga
      961 aaccttcgcc aataaaggcg tactcaatct gcaaagcaat atgccagaca ccaaaccggg
     1021 tctgtatgaa ccgacttcgc caccgattat caccgataaa accatcgtga tggccggttc
     1081 agttaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt ttgatgtcaa
     1141 caccggggag ctgctgtggg cttttgatcc cggcgcgaaa gatccgaacg caatcccgtc
     1201 tgacgaacac acctttacct ttaactcgcc aaactcctgg gcaccagcgg cctatgacgc
     1261 gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct ggggcggtaa
     1321 ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg ccactaccgg
     1381 gaaactggcg tggagctacc agaccgttca ccacgacctg tgggatatgg atcttccggc
     1441 acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccagtta tttacgctcc
     1501 ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgtaat ggcgaactgg tggttccggc
     1561 accggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc ctactcaacc
     1621 attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata tgtggggagc
     1681 caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct atgaaggcat
     1741 tttcaccccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg ggatgttcga
     1801 atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc caatggcact
     1861 gccgtttgtt tcgaaactga tcccgcgtgg tcctggtaac ccgatggagc agccgaaaga
     1921 tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac cgtatggtgt
     1981 cacgctcaac ccgttcctct caccgtttgg tctgccatgt aaacagccag catggggtta
     2041 tatctcggcg ctggatctga aaaccaatga agtggtgtgg aagaaacgta ttggtacgcc
     2101 gcaagacagt atgccgttcc cgatgcctgt accggtgccg ttcaatatgg gtatgccgat
     2161 gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgctgcta cggcagataa
     2221 ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggcc gtttaccagc
     2281 gggtggtcag gcgacgccaa tgacctatga agtgaatggc aagcagtatg tggtgatctc
     2341 cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg cttatgccct
     2401 gccggatgat gtgaaataac cataatgccc ggagagatcc gggcatattt ccaaatacgg
     2461 tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag
     2521 tgcgccatat aagcatgttc tttcggtgca tcgtgattaa acttcaccag cacttcgctg
     2581 acattacctt ctatcttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg
     2641 ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg cagcatcatg
     2701 tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc
     2761 ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc gaacttcccg
     2821 ccatggttca tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc
     2881 attccggcca tcgcctgatc gccatatttc tccattagca tctgcatacc catcatatcg
     2941 agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac
     3001 gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc aatcggctga
     3061 atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg ccatccccat ctggctgacc
     3121 ggtagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac ttcaaaacgc
     3181 tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag accaccgtcg
     3241 ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta
     3301 cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg tgggtagatt
     3361 gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat cacatccagt
     3421 tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac cggaacatca
     3481 tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc
     3541 agcccagcca gccccatcgc cacctgtcgc ccggttttgc cgtgctgatg cggatggaac
     3601 cagcaggtag cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc acctggcgga
     3661 ataattccct gcgggccgcc gtcgacttca cccggtactt ccagcccgtg ccagtgcaac
     3721 gttgtctctt ccgtcagttg gttgtagata tcaaccgtta ccgctttgcc gcgctgtaat
     3781 ttcaccgccg gccccagcag attgccgtta tagccccagg tagttgcagt tttcccgcca
     3841 aaggtggact ggcctgcgcc aatagttaac tgaatgcgat tacgggcatc ggtcgtgagc
     3901 aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga ctgcgcggct ccacagcggc
     3961 aaggctgaag ccacacccag cgcgacggaa tattttaaga aatcacgacg ttgcatagtt
     4021 atttccttat tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc cttcccctta
     4081 cgggaaggtc aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc ctcgctcgca
     4141 gtgtgctaac gtttatcttc tttaagcccc tggtagaagc aatgaagacg tttttcagaa
     4201 cagtgttatt cggcagcctg atggccgttt gcgcaaacag ttacgcgctc agcgagtctg
     4261 aagccgaaga tatggccgat ttaacggcag tttttgtctt tctgaagaac gattgtggtt
     4321 accagaactt acctaacggg caaattcgtc gcgcactggt ctttttcgcc cagcaaaacc
     4381 agtgggatct cagtaattac gacaccttcg acatgaaagc cctcggtgaa gacagctacc
     4441 gcgatctcag cggcattggc attcccgtcg ctaaaaagtg caaagccctg gcccgcgatt
     4501 ccttaagcct gcttgcctac gtcaaataat ccctgatacc tttttgtaga aatattgacc
     4561 gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt tttttacggg tgttaacaaa
     4621 ggaggtatca acccatggcc gaaaaaaaac agtggcatga aacgctacac gaccagtttg
     4681 ggcagtactt tgcggtagat aacgttctgt atcatgaaaa gaccgatcac caggatctga
     4741 tcatttttga gaacgctgca tttggtcgcg taatggcgct ggatggcgta gtacaaacca
     4801 ccgagcgcga cgagtttatc tatcatgaga tgatgaccca tgttccgcta ctggcccatg
     4861 gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg tgccatgctg cgtgaagtaa
     4921 cccgacataa aaacgttgag tcaatcacga tggtggaaat cgatgcgggt gtcgtatcgt
     4981 tctgccgtca gtatctaccc aaccataacg ccggtagcta cgacgatccg cgctttaagc
     5041 tggtgatcga cgatggcgtc aatttcgtta atcaaaccag ccagaccttt gatgtcatta
     5101 tctccgactg caccgatcct atcggtcccg gcgaaagcct tttcacttcg gcattttatg
     5161 aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac ggcgtctgct
     5221 ttttacagca ggaagaagcc atcgacagcc atcgcaaact cagccattac ttcagcgacg
     5281 ttggctttta tcaggcggcg attccgacct attacggcgg tatcatgact tttgcatggg
     5341 cgacagataa cgacgcctta cgccatctct caaccgaaat tattcaggcg cgttttctcg
     5401 cttctggcct gaaatgccgt tattacaatc cggcaatcca tacggcagct tttgccttac
     5461 ctcagtatct gcaagacgca ctggcttcac agccgtccta aggagaagat aagaaattga
     5521 aaaaactgaa actgcatggc tttaataatc tgaccaaaag tctgagtttt tgtatttacg
     5581 atatctgcta cgccaaaact gccgaagagc gcgacggtta tattgcttat atcgatgaac
     5641 tctataatgc caaccgtctg accgaaatcc tgtcagaaac ctgttccatt atcggggcca
     5701 atattcttaa catcgcccgc caggattacg aaccacaggg tgccagcgtc actattctgg
     5761 tgagtgaaga accggttgac ccgaaactca tcgacaaaac agaacacccc ggcccactgc
     5821 cagaaacggt cgttgcccat ctcgataaaa gtcatatttg cgtacatacc tacccggaaa
     5881 gtcatcctga aggcggttta tgtaccttcc gcgccgatat tgaagtctct acctgcggcg
     5941 tgatttctcc gctgaaggcg ctgaattacc tgatccacca gcttgagtcc gatatcgtaa
     6001 ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa cggtatgaag cactttatcg
     6061 accatgagat taattcgatt cagaacttta tgtctgacga tatgaaggcg ctgtatgaca
     6121 tggtggatgt gaacgtctat caggaaaata tcttccatac caagatgttg cttaaagagt
     6181 tcgaccttaa gcactacatg ttccacacca aaccggaaga tttaaccgac agcgagcgcc
     6241 aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg cgcaatatgc
     6301 cagctgttta acggctctgg cggagctccc aggctccgcc agatttattt acttctgctg
     6361 cacgaaattg cggtaagccg ccacgacctg cagaaaatcc tcaacgccgc atagcgacag
     6421 gctttcttcg tcgtagtagt tcatcccctc ttccatttca tcgccagcga attccagttg
     6481 attggcgcga accatcacct cttcaccgtc catccacagg gtgtattcat gccctgcccg
     6541 ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct tgttccactt catcaagcaa
     6601 ggccaggttt tctttcacct cttcattaaa ccagtgcccg accacttcat gccccatgga
     6661 catacgcacc tttaccactc cggtaatatc gcgcagaaat tcgtaatcca tagtgtgttc
     6721 ctctgctccc ggcaatgcgc cgaaatcgca tcgcgccatt gatgtaatta tcgcagcctt
     6781 accggagaaa aacagcggaa cggcgatggt ttagaaataa aaaatgcccg ggaatctcat
     6841 tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaca ccgcagtctg
     6901 gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt tcagataacg
     6961 ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact cttccggcgt
     7021 cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca ggaagacatt
     7081 cgcgccagtc cccagacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc
     7141 gtctgctaca cgcgcctggt tacccataca cagagaacag cccgggatct cgatacgagc
     7201 cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc aactgtgcgg cgtccatacg
     7261 ggttggcggt gccacccaca ggcgggtcgg caactggcct ttatgcgcat ccagcagttt
     7321 acccgcagca cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat
     7381 cttctcgccc tgtaccgcag acagcggacg cgcgtcatcc gggtcgttcg gtgcacagag
     7441 gattggctct ttaatatccg ccagatcgat gtcgatcact gccgcgtatt cagcatctgc
     7501 atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa tacgacgttc
     7561 gagggtacga cgatcgccat aaccttccgc gatcatccac ttcagcagga cgatgttaga
     7621 gttcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag cggcagaacg
     7681 ctcagcggac gcatcggtca gttcaaatgc ctgctcaact ttcagatccg gcagaccttc
     7741 aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa cggtcagcag
     7801 accttgtttg atcgcataca gcgggatcgc gtgtaccaga tcgcgcaggg tgatgcccgg
     7861 ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg gcattacgcc
     7921 tgttgcggct gcaaatgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa
     7981 acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca
     8041 ggagtggatt acgccgtcac ccggacgcag cgacacacca ccacggttca taatgaagtc
     8101 cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tatggcagaa
     8161 agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg
     8221 ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt cgcagtacgc
     8281 gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg ccagcgagaa
     8341 gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt gcggcagacc
     8401 aagtgcttca cgcgctttgg tggtcaggcc acgcccgata atcagcggaa tacgaccgcc
     8461 agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc
     8521 ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt
     8581 caggttagag acgtcgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa
     8641 gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg
     8701 aatatcatcg cccataaacc acagaacgga gttagtggcg gatttacgcg aagaacccgt
     8761 acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcgatttg
     8821 cttgatcgga ccaacaacac ctggctggtc tggctcaata ccttcacggg cgtttttcag
     8881 catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg ccggagaaag
     8941 gtcatcggtg ttagtttcgc cagtgacttt gaagacggta acggtcagtt tttcagccag
     9001 cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcataacct gcttcgcata
     9061 ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca tcagcagcgt
     9121 gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg cgtcgatcag
     9181 cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatggctt tttccggagt
     9241 cagcagaggg gatttggctt cgcctttcgc gatagcagcc aggaagcctg ctttgacata
     9301 ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca ggaattcttc
     9361 ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg gccatttggt ttgcatccag
     9421 gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag
     9481 cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct
     9541 gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt
     9601 tgacgatgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg
     9661 atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc
     9721 tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta tactcggatt
     9781 cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg
     9841 aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctttgt
     9901 gccgcctcta ccgggctata tataaaaagc cgcacagtgg aagcgcctgt ggaaccgcaa
     9961 tcgacacaac agactgcgcc tgacatcacc gcagttacgc ttcctgcaac ggtttccgca
    10021 ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca ctgcacaaat cgatcaatgg
    10081 gttgcgcctg tcgcgctgta ccccgattct ctgctttcac aagtgttaat ggcatcaacc
    10141 tatccggcaa acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc
    10201 gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa atcactggtg
    10261 gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca aaacctgggc
    10321 gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg
    10381 gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac cacaacgaag
    10441 aaagctgtac cggtaacaca gacagtcacg gctcccgtca taccatccaa taccgtttca
    10501 actgccagcc ccgtcattac agagcctgca acaaccgtca tttccattga gcccgccaat
    10561 cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa ctgggccaat
    10621 actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttat tgacagcttt
    10681 gtgcgcggat tcggctatag catgggtgtt gctaccacgt acgcactatt cagcagcatc
    10741 gactgggacg acgacgatca tgaccatcat catcatgacg atgatgatta tcatcaccac
    10801 gatggcggtc atcgtgacgg taatggctgg caacacaacg gcgacaacat caatatcgac
    10861 gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat ggcatggcgg
    10921 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    10981 catcaaaccg atgtcaacgg cggaatgagc gccacgcagc tacctgctcc aacacgcgac
    11041 agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    11101 acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga aaactatggg
    11161 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca acaaaaggac
    11221 gccgctcgtc agcgttatca gtcggcctcg cctgagcagc gccaggcagt ccgcgagaaa
    11281 atgcagacta acccacagat ccagcagcga agagacgcag cgcgtgagcg tattcagtcc
    11341 gcctcgcctg agcagcgcca ggcagtccgc gagaaaatgc agactaaccc acagatccag
    11401 cagcgaagag acgcagcgcg tgagcgtatt cagtcagcct cgcctgagca gcgccaggtg
    11461 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    11521 cgtgttcaat cagcatcacc tgaacaacgt caggtttttc gggaaaaagt tcaggagagc
    11581 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    11641 gcagtacgcg aacgtctctc tgagcgcgga gcaaggcgac aggaaaggta aattgcagac
    11701 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaca
    11761 cttacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg
    11821 ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt gcgatgtctt
    11881 cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttgg
    11941 taccgacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca
    12001 taccgtctgc gcagtcggaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg
    12061 tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt
    12121 ctggttcggt ataggcgatg gacgggataa ctttcggatc gaagtagtgt ttcttaccgg
    12181 cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc atcggctgac
    12241 cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc
    12301 ggatgaaacc acggtcgtcc acttccacgc cagctttgcc tgcgtcgagg tttttaccgt
    12361 tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca gcgggtgctt
    12421 ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg
    12481 tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt
    12541 tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg
    12601 cgtggtatac ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt
    12661 ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg
    12721 gaataaacgg cagttggatc gggcgagaac ccgctgcaat gatcgcgttg tcgaagttga
    12781 tcacggtttt accgttctca ccttcaactt ccagggtgtt agccccggta aatttaccca
    12841 gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct
    12901 gattgatcac tttctctttc caggtacgaa tcttgtcaat atcggttttc ggttcgccga
    12961 agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca
    13021 gtgctttaga agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt
    13081 ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg
    13141 ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat
    13201 atttatctcc ggcggtcata cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc
    13261 ctcaccccag tcacttactt atgtaagctc ctggggattc tggcgcttgc taccttgctg
    13321 cacccagaaa tccatagggt atgtgaagta ataccctaac caccaccggg tcgttctatc
    13381 cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac aaacggcaac cgatttgtct
    13441 attcgctaat aacctcaata cattcatgag attaccagaa aaaagccggc cgttgggccg
    13501 gctcttttac ttacatcacc agacggcgaa tgtcagacag cgtgttgtta atgatggtaa
    13561 tgaaacgggc accatcagca ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca
    13621 gcatcagacg cggcacgaac tctttaccat tccacaccgg ctccatcgcg gacttggaaa
    13681 cgccgaggat agccacttcc ggcgcgttca caatcggcgc gaagtgggta gtacccaggc
    13741 cgccgatgct ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt
    13801 cacgcgcttt cttagaaata gtcatcagct cgcgagacag ctcgatgatg cctttcttgt
    13861 tgacgtcttt gaataccgga acaaccagac cgttcggggt atccaccgcc acaccgatgt
    13921 tgatgtattt cttcagggtc agacgctgac cgtcttccga caacgaactg ttgaagcgag
    13981 gcatctgctc aagagctgca gcaacggctt tcatgatgaa gacaaccggg gtgatcttca
    14041 catccagctt acgtttcgcc gcttcttcgt tctgctgttt acggaacgct tccagctcgg
    14101 tgatatcggt tttgtcgaag tgagtaacat gcgggatcat cacccagtta cggctcaggt
    14161 tcgcaccaga aattttctgg atacggccca gttccacttc ttcgatttca ccaaacttgc
    14221 tgaagtccac cttcggccac ggcagcatgc ctgggatacc gccgccagtc gccgccggag
    14281 ctgcttctgc acgtttgata gcttctttca cgtaagcctg aacgtcttcg cgcaggatac
    14341 gacctttacg gccagtgccc ttcactttcg ccaggttaac gccaaactcg cgtgccagac
    14401 ggcggatcag cggagtcgcg tgaacgtaag cgtcgttttc tgcaaattca gatttgcctt
    14461 ccgctttcgc agccggtgct gctgccggag cttcagcttt tgctgccgga gccggcgctg
    14521 ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa
    14581 tcagcgagcc agttttcact ttatcgccaa cgttgacttt cagttccttc acgacgcctg
    14641 caaacggagc cggaacttcc atagaagctt tgtcgccttc tacggtaatc agtgactgtt
    14701 cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact tcgtcaccgc
    14761 cgatatccgg aacgttaact tctttcacgc cagccgctgg tgcaggggcc gctgccggag
    14821 ccgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact tcgaagacca
    14881 taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct ttcacggtgc
    14941 cagcaaacgg agccggaact tccatagacg ctttatcgcc ttctacggtg atcagcgact
    15001 gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca acttcgtcgc
    15061 tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgctggtgct gctgccggag
    15121 ctgtttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat
    15181 cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact ttgatctctt
    15241 taacgatacc cgcctgcgga gacggaactt ccatagaggc tttgtcgcct tctacggtga
    15301 tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg gtgatttcaa
    15361 cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt ttacctctta
    15421 cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg cgtcagcaac
    15481 cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcta caaccacgta
    15541 ggaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc
    15601 atcagtaccc agtacgcggt agtcgtcagc cggtacgtaa gtacggacct gctcagcgaa
    15661 cagtttcata tagtcggtag atgccactgc cggagcgtcg ttcatcacct gagcgatata
    15721 cggaacgcgc ggagtttcca gcgggtgcag catgttccag cgttcacaat cctgaccatc
    15781 tcgcgccagt tcggtgaagg aggtcacgct ataaacgtca gaacctacgc cgtaatcttt
    15841 cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc ccagcagctg
    15901 aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac ggataccttc
    15961 ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag tgatgtagta
    16021 gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca taatgacagc
    16081 aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca gcgactgaat
    16141 gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac gaccggaagt
    16201 accgccgatc aggaagccac gcgcttgctg gtcgccagcc gcccagcaca gatcgccgat
    16261 acgctggaag ccgaacatcg agtaatagat gtagaacgga atcatcggca gattgttggt
    16321 gctgtaagag gtcgccgctg ccagccagga acaacctgcg cccaactcgt tgatcccttc
    16381 ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg
    16441 ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac cttccatacc
    16501 gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg acttgttctt
    16561 cagcattacg ttcagggcac gaacgaaagc gatagtggta gagatctctt tgctctgctc
    16621 ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg tgaagttcgg
    16681 ctgacggctt ggcagataac cgtgcagttt ctgacgctgt gcgtgcagat aggtatgctc
    16741 ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat cagacaccgg
    16801 cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct taacctggtg
    16861 cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg tatgagcaag
    16921 gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt agattttctt
    16981 cggatcgtga ccaccacggt tcagtgccca gatctgttcg tcagtccagt ctgcaaccag
    17041 tgctgcggtt tccggatatt taccgaagaa gtgttcacga acgtacgcac catctttcga
    17101 tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca gtttaccgct
    17161 ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga tcacgttcca
    17221 gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac cggtgaccgg
    17281 gccgtcaaga cgctgcaggt tacagttgat aacgaagacc aggttatcca gtttttcacg
    17341 ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt cgccgaggaa
    17401 cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat atttcaggaa
    17461 tttagcctgg taaatagcac caatcggccc cagacccata gatacggtcg ggaactgcca
    17521 gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt gaacttcctg
    17581 acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac gagcgtacac
    17641 gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct cgttgcgtgc
    17701 acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga aggacgccat
    17761 gtggccgccc agttcgagat cttttttcga cgcacgcagc accgtcatga tggcgttcca
    17821 gcgaatagct gaacgaatac ggcgttccag ttccagatta cccggatact ccggttgttc
    17881 ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta cgtttacacc
    17941 gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct caacaccttc
    18001 ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc
    18061 atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt gagttttctg
    18121 gaacctgtcc cattgaactc tcgccggaaa gctcaataag acaggttcta cgtttagttg
    18181 ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac cagaaaaatc
    18241 actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc tcttcacgac
    18301 ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt
    18361 cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg
    18421 gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc tgggccaaca
    18481 tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct tcggtgacgg
    18541 caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac tgctgcgcca
    18601 gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta ctacgcagcg
    18661 cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg
    18721 actcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc cataggctgc
    18781 tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct tcgagacgtt
    18841 gaatcgcctc acgcaaggag ggacgggaga cgtcaaactg ttttgccagt tcgcgttccg
    18901 gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc agttgctgct
    18961 caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt tcctgtctta
    19021 agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct aaagtaacaa
    19081 agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg cacattttaa
    19141 caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga ctttacgtag
    19201 gaaaggagtt ttttaacgat tattaaacta taaaaatccg aattgaaccg attcacttac
    19261 caattttgtg atttttaatt caattaaaac gaatttaaat tcattctaca tattgagagg
    19321 ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca accatgcata
    19381 aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt ctttttgtaa
    19441 caattcaaac ttctttgatg taaacaaatt aatacaacaa acggaattgc aaacttacac
    19501 acgcatcact gcgtagatca aaaaaacaac caccgcacga ggtttcatga tggaaggtca
    19561 acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc ttatcgcgct
    19621 gggtggcgcg atagggaccg ggttattcct gggtagcgcc tccgtaatac agtccgcagg
    19681 gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga tcatgcgtca
    19741 gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact ttgcttataa
    19801 atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac tgtacgtttt
    19861 agttgccatg gctgagctga ctgccgtggg taaatacatt cagttctggt atccggaaat
    19921 ccccacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca acctgaccaa
    19981 cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta tcgcggtggt
    20041 agcgatgatc atcttcggcg gctggctgct attcagtggc aacggcggcc cgcaggcgac
    20101 cgttagcaac ctgtgggatc agggtggttt cctgccgcac ggcttcaccg ggctggtgat
    20161 gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc
    20221 agaagctgat aacccggagc aaagtatacc gaaagcaact aaccaggtta tctaccgcat
    20281 cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt ggacccgcgt
    20341 taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata cctttgtggc
    20401 gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca gctgcgtata
    20461 ttgcaacagc cgtatgctgt ttggtctggc acaacagggt aatgcgccaa aagcgctggc
    20521 gtctgtcgat aaacgtggtg taccagtaaa taccattctg gtgtctgcac tggtaacggc
    20581 gttgtgcgta ctgattaact accttgcccc agagtccgct ttcggactgt taatggcgct
    20641 ggtggtatct gcactggtaa tcaactgggc gatgattagc ctggcgcata tgaaattccg
    20701 tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt atccgctggg
    20761 taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga tgaccccagg
    20821 aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta tcggctatct
    20881 gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg ccctcacccg
    20941 tacagggtga gggcaataat ctttacacca acgccccgta aatcgtcagt agtgcaatca
    21001 ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc ggcgtctcca
    21061 ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt aacacctgat
    21121 actgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct ttctcaccat
    21181 gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc agtacatgaa
    21241 gcacggcatc aatgccggac tgtaaacgat gatgcggcgt ctgatatcgc gccagccagt
    21301 attgccatgc acgcaaaaac gcatacccca tcagcgtaac gggtccccag gttcccccca
    21361 caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc aatgcatttt
    21421 gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga atcatggtga
    21481 gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct gtcagataag
    21541 catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc agcagccaca
    21601 ccagtagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca
    21661 cgcccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc
    21721 gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta aacaggcgct
    21781 cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc tgaccagcac
    21841 acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg gcgcaatatc
    21901 acaatggccc gtcatgtttt tagcgatatc cggatagcaa tcaatcagtg cccgcgtaac
    21961 cgccgcaagc tgttgatact gcgcatcggt atacgccagc gtatcggtgc cttcaagctc
    22021 aatcccaata gaaaaatcat tgcagcgttc gcgcccctga tactgagaga ctcccgcatg
    22081 ccatgcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc gaatcaaaca
    22141 gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat
    22201 agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg gcaggctaat
    22261 attgtgcacc accagcaggg tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga
    22321 gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg atctccttat
    22381 atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc gttagctatc
    22441 tggagtttta acatgccgcc tcgccgctat aaccctgaca cccgacgtga cgagctgctg
    22501 gaacgcatta atctcgatat ccccggcgcg gtagcccagg cgctgcggga agatttaggc
    22561 ggaacagtcg atgccaacaa tgatattacg gcaaaacttt taccggaaaa ttctcgctct
    22621 catgccacgg tgatcacccg cgagaatggc gtcttttgcg gcaaacgctg ggttgaagag
    22681 gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga tgacggcgat
    22741 gtcatcaatg ccaatcaatc cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg
    22801 ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag taaggtacgc
    22861 cactatgtcg aattgctgga aggcaccaac acgcagttgt tggatacgcg caaaacctta
    22921 cccggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt
    22981 ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc cggctcagtg
    23041 cgccaggcgg tcgaaaaagc gtcctggctg cacccggatg cgccagtaga agtcgaagta
    23101 gagaatctgg aagaacttga tgaagccctg aaagcaggag ccgatatcat catgctggat
    23161 aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg
    23221 gaagtgtctg gcaacgtcac tgacaaaaca ctgcgtgaat ttgccgaaac gggcgtggac
    23281 tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc aatgcgtttt
    23341 cgctaatagt tgacagattt atccgctcca tcgcgggcgg ataatctccc cttccccaac
    23401 tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg
    23461 aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt tgccaaagta
    23521 gcgccaacta aatcaaggag cgaaacagat ggacaagcaa cgcggtttta cacttatcga
    23581 actgatggtg gttattggca tcattgccat tttaagcgcc attggtattc ccgcttatca
    23641 aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc cttaccgtac
    23701 cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg gtggcagcaa
    23761 tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt
    23821 ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga caccgggttg
    23881 ggataacgca aacggcgtca ccggctggac gcgcaactgc aatattcaaa gtgacagcgc
    23941 attgcatcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag gagcggcaat
    24001 gaatattcca cagctcactg ccctgtgtct gcgttatcat ggagtcttgc tggatgccag
    24061 cgaagaggtg gttcatgttg cggtagtcga tgcaccttcg catgagctac tggacgcatt
    24121 gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac aaatggaagg
    24181 tcacgccagt cgcacacaac agacattgcc cgtagctgtt caggagaagc atcagcccaa
    24241 agcagagttg ctaactcgaa cgttacaatc tgcgctggaa caacgcgcgt ctgatattca
    24301 tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc
    24361 tttaccggat gtttcaccgg atgccggagt cgcattaacc gccagattaa aagtgctggg
    24421 aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc
    24481 aggaaacgcc gtctcatttc gtattgcgac cttaccatgt cggggtggtg aaaaggtggt
    24541 attaaggttg ttacagcagg tgagccaggc actggatgtc aacacgcttg gaatgcagcc
    24601 gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac
    24661 tggccctaca ggcagcggca aaacggtcac gctttatagt gccctgcaaa cgctgaatac
    24721 cgctgacatt aatatttgta gcgtcgaaga tccggttgag atcccaatag ccggactaaa
    24781 ccagacgcaa atccatccgc gtgccggact cacctttcag ggcgtgttgc gtgcgttatt
    24841 gcgccaggat cctgacgtca tcatgatcgg agagatccgc gatggcgaaa cagcagagat
    24901 cgctattaaa gcggcgcaaa ctggtcacct ggtgttgtct accctacaca ctaattccac
    24961 ctgcgaaacg ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc
    25021 gcttacgctg gtaatagccc agcgtctggt acgcaaactt tgcccacatt gtcgccggca
    25081 gcaaggggag cccatccaca ttccagacaa tgtatggcca tcgccgctgc cccactggca
    25141 ggcacccggt tgtgtacatt gctaccacgg tttttatggt cgtacggcct tatttgaagt
    25201 tctgcccata acgccggtca ttcgtcagct tatttccgct aataccgacg ttgaatcgct
    25261 ggaaacgcac gcacgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt
    25321 ggagcaaggc ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcga
    25381 gtaagcaact ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc
    25441 tatgggcaga gagccgtgct ttgctgctca tggcactaca gcaacagatg gttaccccac
    25501 ttagcctgaa gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag
    25561 tcattcatca actggcgacg ctactcaaag ccgggttaac gctttctgaa gggctggcac
    25621 tgctggcgga acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg
    25681 atctcgaaca gggcattgct ttttccaatg ccttattacc ctggtcagag gtatttccgc
    25741 cactctatca ggcgatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct
    25801 ttgaactggc gcgtcagcaa aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt
    25861 tacgttatcc catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt
    25921 ttgttctgcc ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa
    25981 cgcaggggat catgacgctg gcagacttta gtggcgaatg gagctggctg ctggtgttgt
    26041 tcggctttct gctggcgata gccaataagt tgctgatgcg ccgacctacc tggcttatcg
    26101 cgcggcagaa attgttgtta cgaatcccga ttatgggttc actgatgagg ggacaaaaac
    26161 tcacgcagat ctttacgatt ctggcgctga cacaaagtgc aggcattact tttttacagg
    26221 gcgtagagag cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa
    26281 tccagcacga tatcagtaac ggtcatccca tctggctggc gctaaaaaat gccggtgagt
    26341 ttagcccgct ctgtttgcaa ttagtgagaa cgggagaggc atccggctcg ctggatctca
    26401 tgttagacaa cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg
    26461 cagccttact ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg
    26521 tggcgatgta tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct
    26581 ggcgtgttgc tccacgccag cgttgggaga ttacaggttg ttgaagatgc ggttttcttg
    26641 ttcctgcacc cgaataaacg tggtgcgctt ggtcagttct ttcaggcgtg aagccccaac
    26701 gtatgtacaa gctgaacgca agccgcccaa aatatcacgc gcggtatttt caactgggcc
    26761 tcgcagcggc agcttaacgg ttttaccttc tgctgcgcga tattccgcaa cgccgccaac
    26821 gtgacgtttc atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt
    26881 ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcatgac
    26941 gaaatcggca ccgccgccaa aggctttcgc gacatcgccc ggcgtggtgc agccaccgtc
    27001 gctgacgatc attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag
    27061 ctgcggataa ccgacgcctg ttttgacacg agttgtacaa acagaacctg ggccaatgcc
    27121 aactttaacg atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac
    27181 gttgcccgca caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac
    27241 gaagtgttcg gaataaccat tcgccacatc aatacaaacg aagtttaatg ccgggttcag
    27301 gtcgagaatt tgtttagttt tttcgaaatc cgcatcagac gtaccggtag aaaccatcac
    27361 atgtttcagc acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaata
    27421 gtgtttatgc acagcagtca aaatatcaaa agaagccagc gcagaggcca tagaaaatgt
    27481 acctacggtg tccatatttg cggcgataat cggcacgccg gaccagctct gacctgaatg
    27541 tttgaaggtg aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt
    27601 agggcggatg agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg
    27661 attcctgggg ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca
    27721 ctaatcaatg cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt
    27781 tacctggtaa atagtgagcg aacgtttcat caatttcgct ttcgtgcact catttttaac
    27841 gttttgataa tcatgatgaa ttcccgggaa tataaattat gaggtatata gttgccttaa
    27901 cgggaggcat tggcagtggc aagagtaccg ttgccaatgc gtttgctgat ctcggaatta
    27961 acgtcattga tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac
    28021 atgccattgc tgatcacttt ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc
    28081 gggccttgcg cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgccctgc
    28141 tgcatccgct gattcagcaa gagacgcaac accagatcca gcaagctact tccccctatg
    28201 tactgtgggt tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc
    28261 tggtggtgga tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa
    28321 ctcgcgagca tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg
    28381 tggcagatga cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc
    28441 gcctgcacgc acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat
    28501 gcagacccag gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat
    28561 tgagtttttg attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct
    28621 gcatttcttc cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac
    28681 tgagctgttg aaagaacttg accggcagca acgtaaactc cagacctgga ttggcgtgcc
    28741 tggcgtggac cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt
    28801 attaatttcc gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt
    28861 gcgtcagcga ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat
    28921 ttggctgcat ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct
    28981 gaacccgctc acccaggcac ttaccatggt gctggattta attcgccagt cggccccctt
    29041 ccgtaaacaa accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct
    29101 gcgcctgaat ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg
    29161 ttttgccatt cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga
    29221 tttcgaactg gcctgttgct aaggagtgaa aatgtcagaa actattacgg tgaattgccc
    29281 aacctgcggg aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa
    29341 acgttgtcag ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag
    29401 tggcgatctt tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga
    29461 tgccgcacgt aggcctgata agacgcggac agcgtcgcat caggcatctt gtgcaaatgt
    29521 cggatgcggc gtgaacgcct tatccgacct acagacgctt aagcttcgca attaccggtt
    29581 cattggctgg cggaaaatca tcggcattaa gaccgactag cgacatccac tcaccgggtt
    29641 gcccttcttt accccacggc tccccttccc agctttcgac cagccagaac cacagagtta
    29701 tatgcctgtc cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa
    29761 tcccgacttc ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa
    29821 ttttaccgcc gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgcgtta
    29881 taaagatttc attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg
    29941 tcttaaacct ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta
    30001 ttgcaggcga ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt
    30061 acgtcctact ttgcgctcac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc
    30121 atcctgatgg ctaagctgct gcatttgcgc taaacgctcg gcttccatac gacgctgttg
    30181 ttccagctcc tcaacctctt caggcatacg tacctggact ttgctcagcg tgctgataac
    30241 ttcatatttt aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc
    30301 ctgcttcgga tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat
    30361 cgccgccagg tgctctttcc acagagagtc gagagtttgc agcatgacgc ctttttcgaa
    30421 gtgacgcatc atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg
    30481 cgccagaatt cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc
    30541 ggcaattggc aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc
    30601 ccacatttct tccagtgact gtggcggaat gtaggcatca atggtcgctt tgaacacatc
    30661 ttcacgaatg ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg
    30721 ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg
    30781 aatgtcgaag ttacggcttt caactttacg ctgggcgttg gcaatcgctt tagtcaccca
    30841 cgggtgttca atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg
    30901 gtcggaagca aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc
    30961 agcatccccc tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg
    31021 acgctcggta ccgatgatat gcaggccacc tgcttccagt accgcatcgt gacgtacctg
    31081 ccagtcggct ttaatttttt caatttgctc tgcggtcgga ttttccagcg cggcaacttc
    31141 tgcctgccag ctaccaccga gcacaatatc tgtaccacga cccgccatat tggtcgcgat
    31201 agtcaccgca gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt
    31261 ggcgttcagg acgttgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga
    31321 tttttcgatg gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat
    31381 atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc
    31441 tttacgaatc attggacggt tggtcggaac aacgacggta tccagcttgt agatggagct
    31501 aaattcgaaa gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg
    31561 gaagtagttc tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc
    31621 ttctttcgct tccacagcct ggtgcagacc atcggaccag cgacggccct gcatggtacg
    31681 accggtgtgt tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcgcg
    31741 ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc
    31801 cggagagtac agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat
    31861 cagcaccaga ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg
    31921 gccttcgccc tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat
    31981 tttattcacg cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg
    32041 tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt
    32101 acgctgtaca cgttcttcag ggctgaacgc catattgtcg cgcaggtagt caaagccgta
    32161 ttcgttgttc gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg
    32221 catgcccggc aggttgatac cgacagtcag gccaaggaat tcaaacagcg gacggttgtt
    32281 ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt
    32341 tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat
    32401 ttcggcgatg cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg
    32461 cataccaaag acgcgcttac ttgcctcacg taccacggcg aaagcttccg ggatcagatt
    32521 ttccagcact tcgccttttt ccagacgtgc acgaaactct gcggttttcc ctttcagttc
    32581 ttcgtcggag agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg
    32641 catccggcgc agggtgcgat cgttacgact accgaaaact ttagttaaca atttgattag
    32701 cataataaaa tctcaaacgc cccgcgttgc ggagttaata aaatgaagta aaggtttatt
    32761 gttgttaggt gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg
    32821 gcgtgctgaa ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag
    32881 acggcgtgcc ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg
    32941 cctgaagagg caaagattct tcagcaacgg gcagtgtttg cggtgccatt gcgaaagaaa
    33001 gatgacggat taccgtgcga atggcatgtt gatgccagta atcaacggaa tagttcgaat
    33061 tcgggcggcg tgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag
    33121 gctcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg
    33181 caggcaaacc taaactcgcc gcaaccatcc ctaataagag atgcggccag aagtagcgtt
    33241 taccaaactg tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcatatt
    33301 caaacgtatt tagcgcacaa atcttatcat taaagcctgc agtccacagc acgaatgaag
    33361 ataagttgtg cggaaaagtg cttaagaaaa cagcgttcgc accgtttttt atagctctta
    33421 taaaattcaa aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc
    33481 gaattttata cgacagtata aatgtcgtta tgccagtaca gctgaaggcg ctttgaaggc
    33541 caacggcagt tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccag
    33601 gacagcctgc agcagtttgt tattcagtgc atgaccggat ttataagcgg taaatgcacc
    33661 aataatattg tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac
    33721 aaattcgtct tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat
    33781 ggcacaatcg aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg
    33841 catgaaaccg aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt
    33901 catcgcatag cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc
    33961 cagcgaaaaa ccattgtacg gcttaaattc agcccactta tcgccatctt cgacacgaac
    34021 agtctctttg atgcgaacaa attttttggc gcagttcaac tcgtcgatac cggcgtcaag
    34081 cagcaggtat acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac
    34141 ttcgataaca atgttatcga tgcccaagcc cgcgagagca gcattgaggt gctctacggt
    34201 tgaaatccgt acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga
    34261 tttggcatcg gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc
    34321 ggtgttgacc ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac
    34381 acccgtcgcc tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca
    34441 aattacctat ccaaccgaag tgtactatac attcggcggg ccagtttagc acaaagagcc
    34501 tcgaaaccca aattccagtc aattcttaat cagcttgctt acgcaggaat gctgggatat
    34561 ccagataatc cggctctttc gcagtttgcg gcgcattgtc attcacgact ttagcaaccg
    34621 gcttctgctc ctgggtcagc ggagccatcc catgctgctg gtagcgatcc atcactggct
    34681 gctgaacctg cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg
    34741 tcgcaacaac ggttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa
    34801 ccacagtcgc gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat
    34861 ccagacgcag gtcgaagccc gccgtgatgt taaccagcac gccgcgcgcg ccagacaggt
    34921 cgatatcttc cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacggtctt
    34981 caccgctcgc cacgccagaa cccatcattg cgtagcccat ctcagacatt acggtgcgta
    35041 cgtctgcaaa gtccacgttc atcaaacccg gacgagtaat cagttcagcg ataccttgca
    35101 cagcgccttt cagtacatcg ttcgctgcgc caaacgcatc cagcagggag ataccgcggc
    35161 ccagaacttt cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca
    35221 gttcagtgat cccctgctcc gcgaatgcca tacgcttctt gccttcaaag ttgaaaggct
    35281 tagtgacgac agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggtg
    35341 ctgcacctgt accggtacca ccacccatac ccgcagcaat aaagaccatg tctgcacctt
    35401 ccagcgccgc acgcaatgca tcgcgatcct catcagccgc attgcggcca acttctggat
    35461 tagcgccagc gcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg
    35521 ctgttttacg cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatgc
    35581 gctcgcgcac catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa
    35641 tcaccgcgtc attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg
    35701 tcgcctgagg ccgtaatcat cgtcggcctc ataaaaatta aaactctttt cgcagccaac
    35761 tattgagtcg cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt
    35821 caccgttaag atgtgactct ttcccatagt gaagcaatcc caccgccgtc gaataatacg
    35881 gctcctgagc ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacttgcg
    35941 tatgaaacac gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg ccaccggtta
    36001 atacaatgcc tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact
    36061 gcaatatctc ttcgttgacc aggttgagca gctcggtata gcgcggctcg atcacctctg
    36121 ccagggtctg acgttgcaga ctccgcggcg gacgaccacc tacgctcggc acttccacgc
    36181 tctcatcttt tccaacgatg gaacccagcg cacaaccgtg gcgaacttta atcgcttcgg
    36241 cgtcgcttgg cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat
    36301 aaggaattac cttagtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac
    36361 caccaccgat atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat
    36421 aacttgatgc cagtccggca aatatcagtt ggtcaacttt cagcccacaa cgttcaaccg
    36481 ctttgacgat gtttttcgcc atatcgttgt gacatgtgat caggtgcact tttgcctgca
    36541 tccgcacgcc cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact
    36601 cttgcgggat cacatgcagc acacgatgct catcgcgcac acgcaccgat ttcgcggtat
    36661 ggacgacgtt ttccacatct tcttgcgtca cttcttcttc agaaataggc accataccaa
    36721 tttcattctg gcagctgatg tgcttaccag aaagcgccag atataccgaa gagatctgac
    36781 aatctgccat caattctgcc tggtcaatgg cgcgttgtac gcacttgacc acggattcga
    36841 ggtcgttcac cccgccttta tccataccac gtgacgggca gctgcccacg ccaatgatat
    36901 tgaccatacc gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaatct
    36961 ccagtcctac taccagtttt ctgtccgtcg ccttgatcat tgttgttctg cctgtgcctg
    37021 attttgttgc tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga
    37081 gtcataacgc aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa
    37141 aaccggataa agttctacaa agcgagccaa acgtttcatc gtatcgcccc ggccaagatt
    37201 gagcttaata tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca tcgccgcttc
    37261 cttcagagta aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac
    37321 ttcattggcg ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc
    37381 tggcggcacg ctgaaggtgt ttccttccgc gtctaccatg tgttgatcat tccaccgcgc
    37441 aatcggcaca tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct
    37501 cacctgctta atccacggca ggcgttgttc tatttgcgtc tggatgatgt tgacatcctg
    37561 ggtcataaag gtacccggct cacccaatgc caggatcgac tgccggatat cgtcattacg
    37621 cgtgtaatgg cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc
    37681 catccagccc aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa
    37741 aaggatcccc gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcgctgtt
    37801 tcgcgtgttc agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca
    37861 actgcgagaa gctcatacct gcctgacgtg ccgccatcgg caccaggctg tggctggtca
    37921 tacccggtga ggtattggct tccagcagat aaaactgtcc atcgctgtcc agcataacgt
    37981 caatacgtcc ccatcctttg caacctaacg tcgtccatgc tttcagcact aatgcctgca
    38041 aattggcctc ttgtgacgct tccagacctg cggggcagaa atactgtgtc tcatcagaga
    38101 gatacttcgc ctcataatca tagaaggttc cggccggttg aatacgtatt gacggtaaaa
    38161 tttcttcacc gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata
    38221 cttcttcatc gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta
    38281 cttttgacat tcccacactg gaaccttcgc ggctcggctt aacgataacc ggcaaaccca
    38341 gagcagaaat ttctgctaac tgcttatcgc tcaggccttt ttcaaactct gcgcgggtta
    38401 acgctaccca cggcgcgacc ggtaaaccgg caccttgcca cagcagtttg ctgcgtagtt
    38461 tatccattga aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct
    38521 cgagcatgcc ctgcagcgtg ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt
    38581 tctgaaagcc catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggatacg
    38641 catcaatacc gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt
    38701 cccgctcagc ggaagtcccg cccaacagga ccgcgatttt atcagtcatg ttgttcttcc
    38761 tccggagttt gcggcttcag tttgatttca gctaaagaac gggcaatttt tccgatatta
    38821 ccagccccct gaacgagaat caggtcgtta ccggttaata ccggtgccag catctcggct
    38881 acctgcgcag ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga
    38941 cacagcgaac ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc
    39001 atcaacaggg tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta
    39061 cgggtaaaac ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcgcgc
    39121 gccgctttaa tggtggcgtc aacttccgtc gggtggtggc cgtagtcatc gaccagcatt
    39181 gccgtaccgc ttttaccatt cactggctcc agcgggaatt caccgaggaa atcaaaacgg
    39241 cgcccagtcc cctggaagct ctccagcgca cgtaaaatag cctcgtcatc aattccctct
    39301 tccgtagcaa ccgcaaccgc agctgcggcg ttcagcgcgt tatgacgacc tggcgcattc
    39361 agggtgacgc gcatcggctc tttgtcctgg cgcagcagcg taaagtgccc ctgcgggcca
    39421 atctgctgat aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agtcgtggtc
    39481 tgacgtccca ctcgcggtaa caattcgcgg atcaccggat catcaacaca catcaccgca
    39541 cgaccgtaaa acggcaggtt gtgcagaaaa ttaataaaag tctgttttaa attctcaaag
    39601 tcgccctggt aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt
    39661 tgcagatgca ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc
    39721 cccaaacgcg catgaacccc cgccgctttt accagcccgc cgttaacgaa ggttgggtcg
    39781 agccccgctt ctgcgtagat gctggaaacc atcgcggtgg ttgtcgtttt gccgtgcgtt
    39841 cctgcaatgg cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg
    39901 atcaccggaa tacgcgcttc atgagctgcg acaatttccg ggttatcggc agaaatcgcg
    39961 ctggaaacaa cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc
    40021 gtcgccccaa gattcattaa ctgttgcgtg accggatttg gcgctaaatc ggaaccactg
    40081 atctgataac cttcattggc cagaacttcg gcaataccgc ccataccggc accaccgatg
    40141 ccgacaaaat gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc
    40201 aattgttgtg tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc
    40261 atcgctacaa ttacgcccgg gcagcccggc tcacttcatt tgccactcgc tcggtggcat
    40321 ccggaatgga tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc
    40381 cggccagggt gttggcgaca gcatccacgc taagctgtgg ctgctcgata attttggctg
    40441 cgcccgcttt ttccagcggt agcgcattcc agtattgctg gcggtcttta tgttgaaacg
    40501 gcacaaacaa cgccggtagt cctgccgcgg cgatttcact caccgttaaa gcaccggagc
    40561 ggcaaacgac gacatccgcc cacgcatacg ccgccgccat atcatcaata aattccgtca
    40621 ctttatgctg cggttgcccc gcttcggcat acgcctgttc aacggattgt tgcgaacctt
    40681 taccgctctg atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg
    40741 tctggttaag aatgcgtgcg ccctgggaac cacccactac cagcacacga accggacctt
    40801 cacgtccagc caaacgttgc tgcggcaacg gcagcgccag cacatcggta cgcaccgggt
    40861 tacccaccac ttccgcatta gggaaagcgc ctggaaacgc ctgcatcact ttggtggcaa
    40921 tcttcgccag ccatttattg gttaagcccg caataccgtt ttgttcatga agtacaaccg
    40981 gaatgcctaa cgaccacgcg gccagaccac ctggacctga tacatagccg cccataccga
    41041 gtaccacgtc aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga
    41101 tacgcagcgg cgcagctatc agtgctttta taccttttcc acgcagacca gagatacgaa
    41161 tgaaatcaat ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc
    41221 ccagccagcg aacttgccaa ccctgagcca ttagatggtg cgcaaccgcc agtcccggga
    41281 atacatgtcc accggttccg cctgccatca ccattaatcg ctttccttga ccactcatcg
    41341 tgaacctcgt acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag
    41401 catcatgatg gctgtcgaca taatcagtaa gctcgaacca ccgtaactga tcagcggcaa
    41461 tgtcagacct ttggtcggta acatccccgc cgccgcgcct acgttaacca gcgcctggaa
    41521 gctaaaccag atgccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa
    41581 cgctttgcgg ccaatcgaca tcgcgcgaaa agcgacgaag aataccatta aaagtgccag
    41641 caccacaccg acatacccca gttcttcgcc gataatggcg aaaataaagt cagtgtgcgc
    41701 ttccggcaga tactccagtt tttgtaccga gttacctaaa ccttgtcccc aaagttcgcc
    41761 gcgaccaaac gccatcagcg attgcgttaa ctgatagccg ctgccaaagg gatcttccca
    41821 cgggttccag aatgcggtaa cacggcggat acggtacggt tcggcgagta tcagcaacac
    41881 aaccgctgaa atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag
    41941 gaacaacatc gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc
    42001 cagcagtaac actgccaaca ccagaatcac gcccgtcggt ttcaggaagc cgcgcaggtt
    42061 attacgtact tcgtcgcctt tacgcaccag atagttggcg atataacaaa acagcgacag
    42121 ttttgtcagc tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc
    42181 tttaaccgag ctacccacta ccagcacgat catcagcagg atgatagatc cgagcagcat
    42241 cgtggcactg tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat
    42301 gaatgccagg atcagataga cgccatcacg cttcgcgaag aagaacggat cgttggttaa
    42361 gcgttgccct atgggcattg acgccgaggt caccatgata aagccaatcg ccgcaaggcc
    42421 gaaggtcagc cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg
    42481 cgagcccatc acccagcctt ttagcgccgt ggagatccag accaggatac tgaatcctgg
    42541 caggcgcggc attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga
    42601 cgggcaaact cattgcctcg ttgttcaaag ttcttgaact gatcaaggct ggcacaggcc
    42661 ggggagagca gaaccatatc gcccggctga acacgcggag ccagcaagcg catcgcctgt
    42721 tccatagttt cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcaccgtca
    42781 cgaccaaaac agtacaaacg cacgttgtcg ccattcagat agcgcgccag tggactgaag
    42841 tccgccgatt taccatcgcc gcccagcaac aaatgcagcg tgccgtctac ctgcaagcca
    42901 ttcaacgccg cttccgtact gccgacgtta gtggctttcg aatcgttaat ccagcgtacg
    42961 ccattgtgct ccagcaccac ttcaaagcga tgcggcagac cggtgaaggt ggttaacgct
    43021 ttcaggctgc tggcgcgcgg caaaccagct gcgtctgcca gtgccagtgc tgccagcgca
    43081 ttggtgtagt tatgctgccc ggaaagtttc atctctttca cgttcagcac tttctcgcct
    43141 ttcactcgca gccaggtttc tccctgctga tgattcaggt gatagtcacc catgttgacg
    43201 ccaaagctga cgcagcgttc atcagcaccg cgaatcggca ttgttaaggc atcatcagca
    43261 ttgacgacac aaactttcgc attttcgtaa atacgcaatt ttgccgcacg gtattgctgc
    43321 aaaccaaacg gatagcggtc catatgatct tcagtcacgt tcagaatggt cgccgccacc
    43381 gcctgtaagc tggaagtggt ttccagctgg aagctcgaca gttccagcac gtacagttcg
    43441 cactcagcat caagcagcat caacgctggc aggccaatat tgccacccac accaacgtta
    43501 acccccgccg ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa
    43561 ccggtaatcg ccacaatcgg tgcttgtgct tcgcggcaga acagctcgat atcgccaacg
    43621 atttcgattc cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga
    43681 ctggcgacaa tcagatctgc cgccatcagc cattcatcat tcagactgcc cgtgtggcgt
    43741 tctacggctt cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc
    43801 ggcgtcacac cgcgagcgag gaaaaagtcc acgcaggaaa gcccagtgag gcccaggccg
    43861 ataatgacga catttttacc ctgataatca gccatgatta acgtaccttc agcgttgcca
    43921 gaccaatcag aaccagcatc agcgaaataa tccagaaacg cacaatgacg cgcggttccg
    43981 gccagccttt cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc
    44041 gcagtttaaa ggagccgacc tgcaggatga cagaaagcgt ttctaccacg aacacgcccc
    44101 ccataatcac cagcaggaat tcctgacgta gcagtacggc gataatgcct aacgcaccac
    44161 ctaacgccag cgaacctaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca
    44221 ggaagcccag tcctgccccg actatcgccg tgcagacaat gaccagttcc cctgcgtgtc
    44281 gcagatacgg tatatgcaag tagctggcaa agttcatatt gccggtcgcc cacgccacca
    44341 gcgcaaaacc accggcgaca aatacggtcg gcataattgc caggccgtcg agaccatcgg
    44401 tcaggtttac cgcgttgcca gtacccacaa tgacgaagta agccagcaga atgtagaaca
    44461 gccccagctg cggcatcaca tctttaaaga atgggaccac cagctgcgtt gcgggcgtgt
    44521 ctttgccggc aaggtacagg gcgaaggcga cacccagcgc aatgaccgac atccagaaat
    44581 acttccaacg agcgatcaac cctttggtgt ctttacgcac cactttgcga taatcatcaa
    44641 caaagccaat aacaccgtaa cctaccagca ccaccaacac gcaccagacg tacggattgg
    44701 acgggtaagc ccacagcagt acggagatca ctatcgccgt caggatcata atcccgccca
    44761 tggtcggcgt accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc accacctgac
    44821 caaaggaaag tttttgcaaa tgagcaatca tacgcgggcc catccacaat gagatgaaca
    44881 gcgcggtcag caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg
    44941 aataatattt gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaaagc
    45001 gcgtactacc tcttccatgg cggcactacg tgaaccctta actaaaatcg taattacctg
    45061 ttgctcagca atcagtgatt taagacgcgt aattaacgca gttttatcag caaaatgttc
    45121 gccaacgccg ctggcggtgc tgatagcatg gctttgttta cccacgctta acacgcggtc
    45181 aataccagca gcttttgccg cctcgcccac ctgtacatgg caggcttcgc tttcagcgcc
    45241 cagttccgcc atatcgccca ccaccagcac gcggtagccc ggcatttcag ccagtacctg
    45301 aacagcggcg gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt
    45361 ttctgccagt tggatgggga acagacggcc tggaacagct ttcagatttg ccagccccgc
    45421 tttgatagca tcaagcgttg cgcccacgga catggagagc gcagcggctg ccagcgcatt
    45481 cgcaatattg tgacgccccg gcaacggcag cagaacatcg acgctaccgg ttggggtttg
    45541 tagggtaaat tccgtaccgt gcgaggtcac atggatattg gtggcggtga aatcgctgtt
    45601 ggcggcattg ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcag
    45661 ccagtcgttg ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat
    45721 ttcacctttc gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc
    45781 caggttgttg accagcgcag cttccgggcg agtcagactc acagtccagg ctatttcgcc
    45841 ctgatggttc gcgccaagtt caataactgc gtaatcgtat tccggcgtta agcgcaacag
    45901 cgtcatcggt acaccgatgt cgttgttgag attgcctgcc gtataaagcg tgttgccgca
    45961 ctggcttaaa atcgccgccg tcatctcttt aacggaggtt ttgccggagg accccgtcag
    46021 agcaaccacg cgcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag
    46081 acgcgtatcc ttgacgatta actgcggcag gtcgatgtcc agcggacggc taaccagtag
    46141 tgcgcctgcg ccgccagctt tcgcctggtc ggcaaaatcg tgggcatcaa aacgttcgcc
    46201 tttcagggca acaaacaggc agcccggcgt cagttttcgg gtatcagtgg ttacagcatc
    46261 aagggtgata tctgcacctt gcagttcacc gttgagaatg tcggtaagtt ggctaagggt
    46321 tacgctaatc atgcaatcac ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc
    46381 agacgctgat tgccaacaat ctggtaatct tcatggcctt tgcccgcgac cagtaccaca
    46441 tcattctctt tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact
    46501 ttggcatgtc cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgcggttct
    46561 tcggtacgcg ggttatcgtc cgtcaccacc gccacgtcag caaactcttc ggcaattgcg
    46621 cccatcagtg gacgcttacc tttatcgcga tcgccaccac agccaaagac gcaccacagc
    46681 ttgcccgcac agtgcaggcg cgccgcctgt aaggcttttt ccagtgcatc cggcgtatgc
    46741 gcgtaatcca ccaccaccgt cggtttgcct ggcgcagtga acacttccat acgtccgcaa
    46801 accggttgca gacgcgcggc ggttttcagc agatcagcca gtggatagcc gagtgccagc
    46861 agtgtcgcca gcgcgagcag caggttgctg acgttaaaag cgcccatcag atggctttca
    46921 atttcgccat cgccccaact tgagctaaag cgaatcgtcg caccgctgtc gtgatagttc
    46981 acgtcgatcg cttttaacca gcgtccgtga cagttcggat tgatatgatc ttccattgat
    47041 accgcaaccg cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta
    47101 ataatcgcct gaccgcaatg atgttcagaa taaagcagcc atttcgcagc ttcgtagtgt
    47161 tccatatcac catgataatc aaggtgatcg cggcttaagt tggtaaagac cgacgccgca
    47221 aatttcaatg ccgccacacg gtgctgtacc agcccgtggg aggaaacttc cattgcgcaa
    47281 aacgtcgcgc cctgctccac cagccccgcc agctcatgct gaacatcgac tgccgaaccg
    47341 gtagtatttt ctgtcgggat cactttccca agcagaccgt taccaacggt gcccattacc
    47401 gcgctggttt cgccaagcag ttggctccac tgcgccagca gctgggtagt cgtggttttg
    47461 ccgttggtgc ccgttacgcc tacgagacgt aaattgtcag agggttcatg gtaaaagcgg
    47521 cccgccagtg cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc
    47581 atttcacgga tttcaccatc ggtcgcctca tctttcgcct ctgcaataat ggcagccaca
    47641 ccttgcgcta tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct
    47701 acaaagagat cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc
    47761 gaaggtgcgt ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt
    47821 cccctcgcct tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc
    47881 gatgttcatg gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc
    47941 gccgccgtag tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg
    48001 ctgactcgca ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc
    48061 cggcccgacc tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc
    48121 tttcacgccg ccgccgcctg gtagcgccac gctttccatc atatgcacca cggtgcgaac
    48181 aatggattcc gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag
    48241 tgggcgataa atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt
    48301 taccattagc ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt
    48361 ttgaggatat aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc
    48421 aaaacgtgag taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac
    48481 accgacgtta ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac
    48541 gtctttgatt tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg
    48601 caccacgcca cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc
    48661 aaacacgtcg gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt
    48721 agggttgtat gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag
    48781 cacggcgcta ccggattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac
    48841 cagcgcctgc aagcgttcat caatactcaa cgccaggttg tgcgctgcct ggctgtcagt
    48901 agaagaaata tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg
    48961 ctgcccggta agccatttat cgaaactctt ctcaacgccc tcaatcccct gactatcgac
    49021 gttagtaaag ccgatgaggt gagcagtcac ttcgccggat ggataataac ggcgagattc
    49081 ttcacgcaga tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt
    49141 tacctgacgc gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggcagaaag
    49201 ctgatccagc ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat
    49261 accgccagcg tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac
    49321 cgctaacggg cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac
    49381 gcgaagagaa cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca
    49441 cgctacgcgt ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa
    49501 acgccaactg ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc
    49561 tgctttcatg cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc
    49621 aacatgctgc atttgcagtt tttccgtggc gatcctttcc acccggctat ggtcgccgag
    49681 cgcattctct tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac
    49741 cagttgttcg cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacggtcac
    49801 cgccgtcaaa ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc
    49861 gtcaccgata acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt
    49921 gcttagagct tctgtcactc tgctgatcat gcgttcgtcc tctctgcgat acgcagaact
    49981 gaactacggg cacgaggatt ctcagccacc tcttcttcgc ccggcattaa cttgcctaat
    50041 gctcgcagct gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc
    50101 ggaacttgcg gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc
    50161 gagtggaagc tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt
    50221 agcgcctgct ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta
    50281 cgagtcgcgg gatgtttaaa cttatctttc accggcgttg cagcagccac gacttccgcc
    50341 agttctttgg tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg
    50401 cgtttggcaa aacgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct
    50461 gcggtttgta gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc
    50521 ggaccatcgc gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagaca
    50581 ccaagatcga ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg
    50641 cccagcgcgg agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca
    50701 acggcgatag cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc
    50761 gagaggatca gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca
    50821 ggacggatat tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt
    50881 tccatcattt tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg
    50941 ctctgcgtcg atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa
    51001 cttgttgaac tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac
    51061 tggcgcgatt aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag
    51121 taggcgctgc acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc
    51181 gataatttcc cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt
    51241 gcaaaccatt tgaccggcag cgttctcaag cagctgttcc cggtaacggg taggcactga
    51301 taagcgccct ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc
    51361 ccttattccc actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa
    51421 gcttgtcaag ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat
    51481 cagcgttgtt tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag
    51541 aaaaaatccc tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat
    51601 aaagtgtcag tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc
    51661 ctgttgcgtc tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct
    51721 tgttaaatcg gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata
    51781 tcacttaatt attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaac
//
LOCUS       DAZFRN010000016        49978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFRN010000016 DAZFRN010000000
VERSION     DAZFRN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.016"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..679)
                     /locus_tag="ACHKUB_000894"
     CDS             complement(<1..679)
                     /locus_tag="ACHKUB_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFO6653951.1"
                     /translation="MNRQSTATQLQQKSASLSKMLLLIWWLTAAGVWATAGCAWIFQH
                     LHWVTSQKSPGAFVFALAALIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLY
                     TFSSIATVMGITGGMFAAMALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSF
                     SVWLASILTVFIWGITAACEATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVL
                     FRITVTVL"
     gene            complement(824..1528)
                     /gene="ybhL"
                     /locus_tag="ACHKUB_000895"
     CDS             complement(824..1528)
                     /gene="ybhL"
                     /locus_tag="ACHKUB_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO6653952.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1665..2117)
                     /gene="moaE"
                     /locus_tag="ACHKUB_000896"
     CDS             complement(1665..2117)
                     /gene="moaE"
                     /locus_tag="ACHKUB_000896"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO6653953.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(2119..2364)
                     /gene="moaD"
                     /locus_tag="ACHKUB_000897"
     CDS             complement(2119..2364)
                     /gene="moaD"
                     /locus_tag="ACHKUB_000897"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO6653954.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2357..2842)
                     /gene="moaC"
                     /locus_tag="ACHKUB_000898"
     CDS             complement(2357..2842)
                     /gene="moaC"
                     /locus_tag="ACHKUB_000898"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO6653955.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2845..3357)
                     /gene="moaB"
                     /locus_tag="ACHKUB_000899"
     CDS             complement(2845..3357)
                     /gene="moaB"
                     /locus_tag="ACHKUB_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO6653956.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3379..4368)
                     /gene="moaA"
                     /locus_tag="ACHKUB_000900"
     CDS             complement(3379..4368)
                     /gene="moaA"
                     /locus_tag="ACHKUB_000900"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO6653957.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4356..4500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4765..5673
                     /gene="yvcK"
                     /locus_tag="ACHKUB_000901"
     CDS             4765..5673
                     /gene="yvcK"
                     /locus_tag="ACHKUB_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO6653958.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5865..7886)
                     /gene="uvrB"
                     /locus_tag="ACHKUB_000902"
     CDS             complement(5865..7886)
                     /gene="uvrB"
                     /locus_tag="ACHKUB_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO6653959.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8465..9142)
                     /gene="bioD"
                     /locus_tag="ACHKUB_000903"
     CDS             complement(8465..9142)
                     /gene="bioD"
                     /locus_tag="ACHKUB_000903"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6653960.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(9135..9890)
                     /gene="bioC"
                     /locus_tag="ACHKUB_000904"
     CDS             complement(9135..9890)
                     /gene="bioC"
                     /locus_tag="ACHKUB_000904"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO6653961.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9877..11031)
                     /gene="bioF"
                     /locus_tag="ACHKUB_000905"
     CDS             complement(9877..11031)
                     /gene="bioF"
                     /locus_tag="ACHKUB_000905"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO6653962.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(11028..12068)
                     /gene="bioB"
                     /locus_tag="ACHKUB_000906"
     CDS             complement(11028..12068)
                     /gene="bioB"
                     /locus_tag="ACHKUB_000906"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO6653963.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            12155..13444
                     /gene="bioA"
                     /locus_tag="ACHKUB_000907"
     CDS             12155..13444
                     /gene="bioA"
                     /locus_tag="ACHKUB_000907"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO6653964.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            13503..13979
                     /gene="ybhB"
                     /locus_tag="ACHKUB_000908"
     CDS             13503..13979
                     /gene="ybhB"
                     /locus_tag="ACHKUB_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO6653965.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            14131..15414
                     /gene="ybhC"
                     /locus_tag="ACHKUB_000909"
     CDS             14131..15414
                     /gene="ybhC"
                     /locus_tag="ACHKUB_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO6653966.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15648..17909)
                     /gene="ybhJ"
                     /locus_tag="ACHKUB_000910"
     CDS             complement(15648..17909)
                     /gene="ybhJ"
                     /locus_tag="ACHKUB_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO6653967.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIISAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(18092..19525)
                     /gene="ybhI"
                     /locus_tag="ACHKUB_000911"
     CDS             complement(18092..19525)
                     /gene="ybhI"
                     /locus_tag="ACHKUB_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO6653968.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(19601..20653)
                     /gene="ybhH"
                     /locus_tag="ACHKUB_000912"
     CDS             complement(19601..20653)
                     /gene="ybhH"
                     /locus_tag="ACHKUB_000912"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO6653969.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20837..21790
                     /gene="ybhD"
                     /locus_tag="ACHKUB_000913"
     CDS             20837..21790
                     /gene="ybhD"
                     /locus_tag="ACHKUB_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6653970.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(21831..22826)
                     /gene="pgl"
                     /locus_tag="ACHKUB_000914"
     CDS             complement(21831..22826)
                     /gene="pgl"
                     /locus_tag="ACHKUB_000914"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO6653971.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            22981..23799
                     /gene="ybhA"
                     /locus_tag="ACHKUB_000915"
     CDS             22981..23799
                     /gene="ybhA"
                     /locus_tag="ACHKUB_000915"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO6653972.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(23800..24858)
                     /gene="modC"
                     /locus_tag="ACHKUB_000916"
     CDS             complement(23800..24858)
                     /gene="modC"
                     /locus_tag="ACHKUB_000916"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO6653973.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(24861..25550)
                     /gene="modB"
                     /locus_tag="ACHKUB_000917"
     CDS             complement(24861..25550)
                     /gene="modB"
                     /locus_tag="ACHKUB_000917"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO6653974.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(25550..26323)
                     /gene="modA"
                     /locus_tag="ACHKUB_000918"
     CDS             complement(25550..26323)
                     /gene="modA"
                     /locus_tag="ACHKUB_000918"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6653975.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(26489..26638)
                     /gene="acrZ"
                     /locus_tag="ACHKUB_000919"
     CDS             complement(26489..26638)
                     /gene="acrZ"
                     /locus_tag="ACHKUB_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO6653976.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            26767..27555
                     /gene="modE"
                     /locus_tag="ACHKUB_000920"
     CDS             26767..27555
                     /gene="modE"
                     /locus_tag="ACHKUB_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO6653977.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            27623..29095
                     /gene="modF"
                     /locus_tag="ACHKUB_000921"
     CDS             27623..29095
                     /gene="modF"
                     /locus_tag="ACHKUB_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO6653978.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            29356..30372
                     /gene="galE"
                     /locus_tag="ACHKUB_000922"
     CDS             29356..30372
                     /gene="galE"
                     /locus_tag="ACHKUB_000922"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO6653979.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            30382..31428
                     /gene="galT"
                     /locus_tag="ACHKUB_000923"
     CDS             30382..31428
                     /gene="galT"
                     /locus_tag="ACHKUB_000923"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO6653980.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPNHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            31432..32580
                     /gene="galK"
                     /locus_tag="ACHKUB_000924"
     CDS             31432..32580
                     /gene="galK"
                     /locus_tag="ACHKUB_000924"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO6653981.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTILAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            32574..33614
                     /gene="galM"
                     /locus_tag="ACHKUB_000925"
     CDS             32574..33614
                     /gene="galM"
                     /locus_tag="ACHKUB_000925"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO6653982.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            33819..34571
                     /gene="gpmA"
                     /locus_tag="ACHKUB_000926"
     CDS             33819..34571
                     /gene="gpmA"
                     /locus_tag="ACHKUB_000926"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO6653983.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(34729..35781)
                     /gene="aroG"
                     /locus_tag="ACHKUB_000927"
     CDS             complement(34729..35781)
                     /gene="aroG"
                     /locus_tag="ACHKUB_000927"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO6653984.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHAPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            36097..36477
                     /gene="ybgS"
                     /locus_tag="ACHKUB_000928"
     CDS             36097..36477
                     /gene="ybgS"
                     /locus_tag="ACHKUB_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO6653985.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            36591..37532
                     /gene="zitB"
                     /locus_tag="ACHKUB_000929"
     CDS             36591..37532
                     /gene="zitB"
                     /locus_tag="ACHKUB_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO6653986.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(37529..38248)
                     /gene="pnuC"
                     /locus_tag="ACHKUB_000930"
     CDS             complement(37529..38248)
                     /gene="pnuC"
                     /locus_tag="ACHKUB_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO6653987.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(38286..39329)
                     /gene="nadA"
                     /locus_tag="ACHKUB_000931"
     CDS             complement(38286..39329)
                     /gene="nadA"
                     /locus_tag="ACHKUB_000931"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO6653988.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(39578..39653)
                     /locus_tag="ACHKUB_000932"
     tRNA            complement(39578..39653)
                     /locus_tag="ACHKUB_000932"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39618..39620),aa:Lys,seq:ttt)
     gene            complement(39687..39762)
                     /locus_tag="ACHKUB_000933"
     tRNA            complement(39687..39762)
                     /locus_tag="ACHKUB_000933"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39727..39729),aa:Lys,seq:ttt)
     gene            complement(39909..39984)
                     /locus_tag="ACHKUB_000934"
     tRNA            complement(39909..39984)
                     /locus_tag="ACHKUB_000934"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39949..39951),aa:Lys,seq:ttt)
     gene            complement(39988..40063)
                     /locus_tag="ACHKUB_000935"
     tRNA            complement(39988..40063)
                     /locus_tag="ACHKUB_000935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40028..40030),aa:Val,seq:tac)
     gene            complement(40214..40289)
                     /locus_tag="ACHKUB_000936"
     tRNA            complement(40214..40289)
                     /locus_tag="ACHKUB_000936"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40254..40256),aa:Lys,seq:ttt)
     gene            complement(40292..40367)
                     /locus_tag="ACHKUB_000937"
     tRNA            complement(40292..40367)
                     /locus_tag="ACHKUB_000937"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40332..40334),aa:Val,seq:tac)
     gene            complement(40503..40578)
                     /locus_tag="ACHKUB_000938"
     tRNA            complement(40503..40578)
                     /locus_tag="ACHKUB_000938"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40543..40545),aa:Lys,seq:ttt)
     gene            complement(40743..41534)
                     /gene="cpoB"
                     /locus_tag="ACHKUB_000939"
     CDS             complement(40743..41534)
                     /gene="cpoB"
                     /locus_tag="ACHKUB_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO6653989.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(41544..42065)
                     /gene="pal"
                     /locus_tag="ACHKUB_000940"
     CDS             complement(41544..42065)
                     /gene="pal"
                     /locus_tag="ACHKUB_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO6653990.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(42100..43392)
                     /gene="tolB"
                     /locus_tag="ACHKUB_000941"
     CDS             complement(42100..43392)
                     /gene="tolB"
                     /locus_tag="ACHKUB_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO6653991.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(43525..44790)
                     /gene="tolA"
                     /locus_tag="ACHKUB_000942"
     CDS             complement(43525..44790)
                     /gene="tolA"
                     /locus_tag="ACHKUB_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO6653992.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(44855..45283)
                     /gene="tolR"
                     /locus_tag="ACHKUB_000943"
     CDS             complement(44855..45283)
                     /gene="tolR"
                     /locus_tag="ACHKUB_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO6653993.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(45287..45979)
                     /gene="tolQ"
                     /locus_tag="ACHKUB_000944"
     CDS             complement(45287..45979)
                     /gene="tolQ"
                     /locus_tag="ACHKUB_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO6653994.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(45976..46380)
                     /gene="ybgC"
                     /locus_tag="ACHKUB_000945"
     CDS             complement(45976..46380)
                     /gene="ybgC"
                     /locus_tag="ACHKUB_000945"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6653995.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(46530..46823)
                     /gene="ybgE"
                     /locus_tag="ACHKUB_000946"
     CDS             complement(46530..46823)
                     /gene="ybgE"
                     /locus_tag="ACHKUB_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO6653996.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(46823..46936)
                     /gene="cydX"
                     /locus_tag="ACHKUB_000947"
     CDS             complement(46823..46936)
                     /gene="cydX"
                     /locus_tag="ACHKUB_000947"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO6653997.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(46951..48090)
                     /gene="cydB"
                     /locus_tag="ACHKUB_000948"
     CDS             complement(46951..48090)
                     /gene="cydB"
                     /locus_tag="ACHKUB_000948"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6653998.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(48106..49674)
                     /gene="cydA"
                     /locus_tag="ACHKUB_000949"
     CDS             complement(48106..49674)
                     /gene="cydA"
                     /locus_tag="ACHKUB_000949"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO6653999.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        12310 a        13311 c        12377 g        11980 t
ORIGIN      
        1 caagaactgt tacagtaatt ctaaagagga cgcttaaaat actaaaatag agtgttattg
       61 ccccaggaac gatacagcga agagaacctg aaatttcaga ggtgtcggca acacggatca
      121 aatcttccag tgttgttgct tcacaggcag ccgtaatgcc ccagataaag acagtcaaaa
      181 tactcgctaa ccacacggaa aaactgctgg taaggatcag gttaacaaca aaagcaatac
      241 tcaaaccaca gaaaatatat gaatataact gtctgactgg tgggataacc ctattactac
      301 aagagcaaat cagcgccatt gcggcaaaca tccctcccgt aattcccatt accgtcgcta
      361 tgctcgaaaa ggtataaagg ctggcgaaga gaaagccaac aagaaggatt attagcagat
      421 agaccagaca tgcgcctgat acattcagtc ttttcagggc gttgtttaac cagagaccac
      481 ctattatcag cgcagccaaa gcgaagacaa acgcacctgg agacttttgt gacgtaaccc
      541 aatgcaaatg ctgaaatatc caggcacagc ctgccgtagc ccatactcca gctgctgtta
      601 accaccaaat gagaagcagc attttgctga gcgaagcact tttttgctgt aattgagtag
      661 cagtggattg tctgttcaaa tgcgttatct gtccctgaaa ttacgtagtg gacatagtct
      721 ggcatttgcc tcgattcagt tctcatgtaa atgagcggaa tagtctttat aaaatccgaa
      781 aaaaatcccg gctccaggcc gggatcagca ggaagaagag aatttaacgg cggttgccga
      841 agatccgcaa caacatcagg aacaggttga tgaagtccag atacaaggtt aacgcgccaa
      901 gaatggaata tttgcgcagg ttcgacgtgt cgcgggtatc aatctgctca cccatatttt
      961 tcagtttctg cgtgtcatac gccgtcaatc cgacaaagac aatcacgccg atgtaggtaa
     1021 ctgcccacat caatgcttcg cttttcaacc agaagttgac cagcgatgcc agcacaatgc
     1081 cgattaacgc cataaacagc atattgccaa agccacttaa atcgcgcttc gtggtgtaac
     1141 cgtacaggct cattgcgccg aacatcccgg cagtaacgac gaaagtactg gcgatagaag
     1201 cagcggtata gacaatgaat atactggaaa gcgtaagacc cgtcagcgcc gaataaagca
     1261 taaagagcat cgtcgttaca cctgcgctca gcttttgaat catcgctgat aacacaataa
     1321 ccaatgctaa ttgcgcgatg atcagaccga ttaaaaagac acggttagtg aacaacagct
     1381 ccatcacggc cgcggaatta gccgcatacc aggcaacaaa tgcggtcagc aacaagccaa
     1441 cggtcatcca gccatagact tgcgccatat aagtttgcaa gccagcccgg ggttgtacga
     1501 ttgaatcaga acgtgggaat ctgtccatga cgatctcctg aagatataag gaatatctta
     1561 aggatactgc aaaatgatga ggctgtgcat cgacgcagcg taaacgtatg tactgagcgg
     1621 tgaaattgcc ggacgcagcg gtgccttatc cggctaacaa aaaactacca gcgttttgcc
     1681 gcctgctgat cgctctcccg ggcttcaacc cagcggtcgc cttccggcgt ggcttcgcgc
     1741 ttccagaacg gtgcgcgggt tttgagataa tccataataa actgcccggc ttcaaacgca
     1801 ctgctgcgat gcgcactggt gacaccgaca aaaacgattt catcgcccgg ccataattcc
     1861 ccgatgcggt gaatcacagt gacgcgcccc agcggccagc ggttacgcgc ttcatcaaca
     1921 atttctgcca gtgctttttc agtcatcccc ggatagtgtt cgagggttaa tgctttgacg
     1981 ctgtcgccca ggttatggtt gcgcacctta ccagtaaagg tgactaccgc accgtcttcg
     2041 tcacgctccg ccagccacgg gtactcttct cctacgctga acggctgcgg accaacaaca
     2101 attttggttt ctgccatctt aacctccggt taccggcggg aagaaagcta cttcgtcgcc
     2161 gtcagtcagc ggatggtcaa aactcaccag cgtctggttg acggcagcca gtaatttgcc
     2221 atcttccagc gccagcgccc agcgatcgct ctgcgcagcc atgtgctggc gtaacgcttc
     2281 aacagttggg aaatccgcag ccacttcggt tgcatctgtt cccaccaact cgcgcacctg
     2341 ggcgaaaaaa agaactttaa tcatccgctt ccaccttaaa gtcacccgac ttgccgccgc
     2401 tcttcgccag caaacgtacc ggaccaatca ccatatcttt ttgcaccgct ttgcacatgt
     2461 cataaatggt cagcgccgcc acggaggccg cggttaatgc ttccatttcg acaccggttt
     2521 tcccggtcag gcggcataag gtttctatac gcacccgatt gtgctccggc tcggcctgta
     2581 aattgacttc aactttgctg agcatcagcg gatgacagag cgggatcaga tcccaggtgc
     2641 gttttgccgc ctgaataccg gcaatacgcg cagtggcaaa tacgtcgcct ttgtggtggc
     2701 gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt gacaaaggct tccgcccgcg
     2761 cttcacgcac ggtttccgct ttggcggaga catccaccat gtgcgcttcg ccagcggcgt
     2821 tgatatgggt cagttgcgac atacttattt cttcaaatgt ggatggaaat tacacggacg
     2881 cgtacgggca tccagctgcg gcgcgatgat attttcccat gcggtacggc acgctttggt
     2941 cgaacccggc atggcgaaaa tcagcgtttt gttggcgacg cccgctaccg cacgagattg
     3001 caacgtggaa gtgccaatct cttcaaaaga caacatccgg aacacttcac caaaaccttc
     3061 aacttcacgg tcgaacaacg gcagcaatgc ttcgggagcc tgatcacctt ccgtcaggcc
     3121 agtaccaccc gtaatcaata ccacttgtac atcgtcgctg gcgatccacg cagatacctg
     3181 agcgcgaata gcgtagcggt tttctttcac aatggcttta tcgacaacgt gatggcccgc
     3241 ttcttgcgcc gaatcgcgca gatagtgacc ggaggtatcg tcttcttcac cgcgacgatt
     3301 agaaaccgta agaatagcaa tacgggtcgg gataaattca gtgcttacct gactcatctg
     3361 atctctcctt ttgacgtttt agccgccaat gtacgataag ttttgcgtaa taccggtgtt
     3421 gttttgatgc aggaaatggg tctgtttctt ctcccgcagc gccgctgaaa tacgcgcttc
     3481 cagcgcctgt tgttgggtat cgtcttccag cagatcgcgc aggttaacgc cgccttcacc
     3541 aaacaggcag agatggagtt taccaatgga ggaaacgcgc aggcggttgc aagtggcgca
     3601 gaagtctttt tcatacggca tgataaggcc aatctctccg gcgtaatctg gatggcaaaa
     3661 gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg atccagccgc gacgcagtag
     3721 ctcgtcacgc agaacctgac cagagatgtg atgcttacgg aagagctcgc tgccctcgcc
     3781 cgtttccatc agttcgatga aacgcagctg gatagggcga tgctggatcc agttcagaaa
     3841 ggtgtcgagc tggtgatgat taacatcacg catcagcacg gtattgactt tgaccttctc
     3901 aaaaccggcc tcaaatgcag catcaatccc cgccatgacc tggttgaatt tatcctgccc
     3961 ggtaatagcg tgaaactggc gggcgtccag actgtcgaca ctgacgttaa tgccagtaag
     4021 tcccgcatcg cgccagttcg ccacatcgcg ttccagacgg taaccattgg tggtgaccgc
     4081 aatctggcgg atagcgtcgt tttcccgcac agcggcgatg atatcggtaa agtcgcggcg
     4141 taaagacggc tcaccgcctg tcaggcgcac tttttcggtg cccagactgg cgaaggcgcg
     4201 cgtaacccgg cgaatttcat cgacggtaag aaagccttta ttggtgacgc cgctcggttt
     4261 gtagccatcc ggcaggcagt aggtgcaacg aaagttacac acatcggtaa tcgacaggcg
     4321 caagtagtaa aacttacgcg caaatgcatc agtcagttgt gaagccatgt acacctttcc
     4381 agatacggga ggcgaagtca tttcttcctt cgccctggtg gcaatcttta cgagcattgt
     4441 cacggccaaa gcaccgtatc agttgaccca ggtgcagagg ctagagtgtt tagtggttat
     4501 gccgatacta gcgtgtaaat gagattttta ccatccacac tttcgctatg taatcatgta
     4561 tatagcgtca tgatcgtgct atttcgctac agaataaggg aaaatggcgg ttttactttg
     4621 atatacatca tcagggaggc atagcgtgca tcgtccttta gaggtagaag aaaatagttt
     4681 gtatcctcaa tattggcagg ttaattgctg tttccccgca atttgcgcta ctgtagcgcg
     4741 gataatctga ctccaggaaa ctatatgcgc aatcgtacgc tggctgatct tgatcgtgtc
     4801 gttgctctcg gcggagggca tggactggga cgcgttctct catcactttc gtctttgggt
     4861 tctcgtttaa cgggtatcgt caccaccacc gataatggtg gctcgacggg gcgtattcgc
     4921 cgttcagaag gcggcattgc ctggggcgat atgcgcaact gcctcaacca gctgataacg
     4981 gaaccgagcg tcgcctccgc gatgtttgaa taccgttttg gtggcaatgg cgaactttcc
     5041 ggtcataatc tcggaaactt gatgttaaag gcgctggatc accttagcgt gcggcctctg
     5101 gaagccatca atttaattcg taatctgctg aaagtggata cgcatttgat tccaatgtca
     5161 gagcatcctg ttgatctgat ggcgattgac gatcaggggc atgaagttta cggcgaggtc
     5221 aatatcgacc agttaactac gccgattcaa gagttattgt taacgcctaa tgtacccgca
     5281 acgcgtgagg cggttcacgc tatcaatgaa gcggatctca tcattattgg gcctggcagt
     5341 ttttatacca gcctgatgcc aattctgctg ctgaaggaaa tcgcccaggc attacgccgc
     5401 acgccagcgc cgatggttta tatcggcaat ctggggcgtg agttgagttt acctgcggct
     5461 aatttgaagc tggaaagcaa gctggcaatt atggagcagt atgttggtaa aaaggtcatt
     5521 gatgcggtca tcgtcgggcc aaaagtggat gtctcggcgg tgaaagagcg gattgtgatc
     5581 caggaggtac tggaggccag cgatattccg tatcgtcatg accgccagtt gttacataac
     5641 gcgctggaaa aggcgttaca ggctttaggt taacggaaat ccgaaggaaa attccggctt
     5701 cctattgaag acaaagtgcg cgttgtttat gccggatgcg gcgtgaacgc cttatccggc
     5761 ctacaaaccg cgcaaattca atatattgcg gagaaaatgt aggcctgata agcgtagcgc
     5821 atcaggctgt tttccgtttg tcatcagtct tcttcgctat cctgttacga tgccgcgata
     5881 aacagctcac gcagctgatg caactggtca cgaatttgcg ccgcttcttc gaactccaga
     5941 ttctgcgcgt gttgcatcat caacccttcc agctcatgga ttttctgctg caacgcttta
     6001 ggcgacatat ccatcggcac attatccggc tcaacaatcg gtcgcgattt tcctctgccc
     6061 ttcgctttgg ttttagcaat gttctgcccc aacgccagga tatcgaccac tttcttgttc
     6121 aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc gcggcgacgt
     6181 tcggtttcgc caatcgcttt cgccattgat ggggtgatct tatcgccgta gagaatcgct
     6241 ttaccgttaa cgttacgtgc cgcacgacca atggtctgga tcaacgaacg ttcggaacgc
     6301 aggaagcctt ctttgtcagc gtcgaggatc gccaccagcg aaacttccgg catatccaga
     6361 ccttcacgca gtaagttgat ccctaccaat acgtcgaact cacccagacg caagtcgcgg
     6421 ataatctcca tacgctcgac ggtgtcaata tctgagtgaa gataacgcac gcgctcaccg
     6481 tgttcttcga gatattcggt aagatcttcc gccattcgct tggtcagtgt ggtgaccagt
     6541 acgcgttcgt taattgccgc tcgctgacga atctccgaaa gaagatcatc aacctgtgtc
     6601 gccaccggcc gcacttcgat aatcgggtca agcaatccgg ttggacgcac cacctgatcc
     6661 accacatcgc cgccggattt ttccagctcg taattacccg gcgtcgccga aacatagatg
     6721 gtttgcggcg ctaatgcttc gaactcttca aacttaagcg gacggttatc cagcgctgat
     6781 ggcaggcgga agccgtactc caccagtgtc tctttacgcg cccggtcacc gcgatacatg
     6841 ccgccaattt gtggaatggt gacgtgagat tcatcgacca ccagcagccc atcagcaggg
     6901 aggtaatcaa acagcgtcgg cggtggctca cccggtccac gaccggagag gaagcgcgag
     6961 tagttttcaa tccccgaaca gtagcccagc tcgttcatca tctccagatc aaactgggta
     7021 cgctgggtca gccgctgctc ttccagcagt ttgttgtttt ccaacagcac tttgcgtctg
     7081 gcggccagct cttctttgat ctcctccatc gcctgtacga tgcgctcgcg cggtgtgacg
     7141 tagtgcgttt tcgggtagat ggtaaaacgt ggaatagtgg aaacaatctg cccggtcagc
     7201 gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca gttccacgcg aagtgcaatg
     7261 tcatccgatt ctgccgggaa gatatctatc acctcgccac gaacgcggaa agtaccacgc
     7321 tggaatgctt gatcattacg agcgtattgc agctccgcca gtcggcgcag aatcgcgcgc
     7381 tgatcgataa tcataccgac cgtgagatgg agcatcatct tgagatataa atcaggatcg
     7441 cccagaccat aaatcgcgga aacagacgcc accacaacca catcacgccg ctccagcatc
     7501 gctttggtgg cggacaaacg catctgctca atatgttcgt taaccgaggc atctttctca
     7561 atgaaagtgt cggaactcgg tacataggct tccggctgat agtagtcgta gtaggaaacg
     7621 aaatattcca ccgcgttttc cgggaagaac tctttcattt cgccatacag ctgggccgcc
     7681 agcgttttgt tgggcgcaag taccatggtt gggcgctgaa ggtcagcaat gacattggca
     7741 atggtgaatg ttttcccgga gccagtcacg ccaagtaacg tctggtgcgc caggccatct
     7801 tccagcccct cttcgagacg tcgaatcgcc tctggctgat cgccagaagg tttaaaagcg
     7861 gaattcagtt tgaacggttt actcatgagt cgctacctga aggagttggg cgggcaggta
     7921 tgtaatttta ctcgtcgtac ttaattatgc caacaaatta tactggataa aaaaacagtt
     7981 catcaccata atatttctgt tacagcgtaa actccgctgt caatcatgag caaaatttac
     8041 tctgtggcga gataaaactc cggccttacc gggttatccc caaagcaacg gcttttttaa
     8101 catttgtcaa gatgagtgat gacagtttta tggcaagaga tgcctgttca gtgactgcca
     8161 ttgcatttat ctaaccagtt aaaaaataaa agatatttct ttgagccgtc tttaacgcca
     8221 attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt tttctaactc taatacacat
     8281 ggttatccac aggaatagtg gataactgtc tccagcccct atcccccgcc gcctgggcaa
     8341 ttcccacatt cgccgcatga ggcggaagta aaattttttt cgtgattatt ttagaattta
     8401 attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc gcaatttaac caaatacagg
     8461 atagctacaa caaggcaagg tttatgtact ttccggttgc cgcattttct ggattttctg
     8521 caagccaggg gatctctccc agcagcggcg cgggaatcat gcgggtgagc gtggtcatat
     8581 attcagcgtg acgttttccc ggaggcgtaa catcgttcgc cacccaaccc gccagagtca
     8641 gtccggcgtg ttgtattgcc tgcgcagtca acatcgcgtg attaatacag ccgagtttca
     8701 caccaactac cagtatcacc ggcagttgtt cctgtgttac ccaatctgca aaagtgaaag
     8761 tgtcagaaag cggcgtaaac cagccgccag caccttccac taacacccag tcagcctgtt
     8821 gttcaagcgc gcgtaatccg gcgctcatta ccaatgattc tatcggcctg ccctcttgcg
     8881 cgctgatgat gtgcggcgag gtgggttctg cgaaggtgta aggatttacc gttgcgtaat
     8941 ccagctgcag gctgctgttg tgctgtaacg ccagcgcgtc actattacgt aagccttccg
     9001 gggtcttttc gctgccagag gcgaccggtt tataacctgc cgtccgataa cctactgcct
     9061 tcgcggcttg taaaagtgca caactggcga cggttttccc cacttcggta tccgttccgg
     9121 tgacaaaata acgtttactc acgagcaatc actcctaaaa aaagatgata cgtcagagga
     9181 tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct gcaattgcga acgcgttaat
     9241 attcgcgggt cgcgcccttc atgaagatgc gtagcaccga tgcctttcag cgaacgcatg
     9301 gcactgagcg catcatcaaa ccatagtgtg atgggctgaa tatgatgttg ataatgcacg
     9361 ccgttcagcg actgttcgat ttcatctggc ggtaaaaagc gattagcatg cggacgctcg
     9421 tccaccgcct gccacgcctg atgcagttcg ggtaacgatc cctgcaccag cgtggtaaac
     9481 gccaccacgc ctccggggcg caccacccga tacagctcgc ggagtgccgt ggataaatta
     9541 ccgcaccact gcactgcgag attgctccat gcaagatcga acgtcgcagt cgctaacggc
     9601 agggattcga tatctcccgc cagataatgg tctgcggcat ccttctggcg tgcctgaaca
     9661 agcattggcg gcgagagatc taaggccgtc acctgcgcgt gacgttcccg ccagtggcgg
     9721 ctcatccagc caggtccaca acccgcgtcc agtacgtggg tgtatttacg ctgtgggagc
     9781 attgccagta agacgtcagc actctggcgc tgtagatctg cgtgttgctc atagagtgcg
     9841 gctgcccgac caaatgccgc tgcaatggct tgtttattaa ccgttgccat gcagcacctc
     9901 cagcagacgg tcgatatcct gcatttcatg cgctgcggtt agcgttaagc gcagtcgcgc
     9961 agtaccagcg ggtacggttg gcgggcgaat cgccgtgacc cagcagcctt gctggcgcag
    10021 tttttctgcc agttgtaacg cacggctgtt atcaccgacg atcaatggct ggatggcgct
    10081 gcatgaatca gccagcgtaa acggcaaatc ctgtactccg gcacgaaaac gcgcaatgag
    10141 tgacaccagt ttttcgcgcc gtgcatcccc ctcatcacgg cgaatgaccg ccagtgacgc
    10201 acgtaatgcc tgcgcctgag cgggcggcat actggtgctg tagataaggt ggcgggcgaa
    10261 ttgcagcaga taatccgcca ccgtactgga gcaaagcacc gcagccccac tgacgccaaa
    10321 tcctttgcca aaagtcacca ccagcaattc tggttttacc ttttgcagcc agcagctgcc
    10381 gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca tcgaccatca accagccatt
    10441 gtgctgttgc gttacctgct ggatttccgc cagtggcgca atatcgccgt ccatgctgaa
    10501 cacgccttct gtcaccacca gttgctgccc cggacagggg gaagcaagca gtcgcgccag
    10561 atgagtgaca tcgttatgaa caaaacggcg aagctgcgac gggcttaaac tggcagcctc
    10621 cagcaatgag gcatggctaa gccggtcagc gacaatgcgg tcctcttttg ccatcatcgc
    10681 ggcaatgacc gcctgattag cggcaaaacc agagataaat agcagtgccc gcgaatagcc
    10741 cagccactcg gccagttctt cttccagcgc ctgatgcgca acgctataac cgctgacgtg
    10801 accggaaccg ccgctaccga cgccaaattg ctccgcaccc tgctgccagg cacggataat
    10861 ttgcggatga tggcttaaac ctaaataatc gttactggaa aagttcagat actggcgatc
    10921 atccgccacc agccagcgtc cggctccttg cgccactgga taacggcgac gcagggcatc
    10981 ggcagcacgc cgcgcatcga gcgccgcgtt gattttctcc tgccagctca tagtgctgcc
    11041 gcgttgtaat attcgtcagt gtccggggtc atcagtgcct gctccagacg ctgctgttgt
    11101 tcgttatccc ctgccagcac ggcagtttgc tgcggattta gccccagttt gcgaaacagt
    11161 tgcaggtctt tatcttcttc cggattcggc gtggttagca gtttgcaacc gtagaaaatc
    11221 gagtttgcgc ctgccataaa gcacatcgcc tgagtctgtt cgttcatctg ctcgcgtccg
    11281 gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg cgaccgcaat ggtgcgaata
    11341 aaatcaaagg catcgacatc atcgttatcc gccagcggcg tgcctttcac cttcaccagc
    11401 atgttgattg gtacgctttc cggcggcgtc ggcaggtttg ccagttgtag caataatccg
    11461 gcgcgatctt ttaccgtttc gcctaagccc acaatgccgc cagaacagac tttgatcccg
    11521 gcatcgcgca ctttttccag cgtatcgagg cgttcctgat aagtgcgggt ggtgatgata
    11581 ttgccgtaaa actccggcga ggtgtccagg ttgtggttgt agtaatccag cccggcgttc
    11641 gcgaggcgct gcgcctgaga ttcactcaac gtgcccagcg tcatacacgc ctccagcccc
    11701 atcgctttta ccccctgcac catttgttcc aggtacggca tatcgcgttc gtggggattc
    11761 ttccacgccg cgcccataca gaaacgcgtc gatcccgcag ctttcgcttt gcgcgccgac
    11821 tccagcacct gttcaacttc catcaaccgc tcggcttcca gcccggtttt gtagcgcgag
    11881 ctttgcgggc agtatttgca atcttccgga caagctccgg tcttaatcga cagcaaagtg
    11941 ctgacctgca cctgacgagg atcgaaatgc tgtcgatgca cctgctgcgc ttcaaacagc
    12001 agatccagca acggtttttc aaataattct gtgacttgcg acaatgtcca gcgtgggcgg
    12061 tgagccatgg ggcttctcca aaacgtgttt tttgttgtta attcggtgta gacttgtaaa
    12121 cctaaatctt ttcaatttgg tttacaagtc gattatgaca acggacgatc ttgcctttga
    12181 ccaacgccat atctggcacc catacacatc catgacctcc cctctgccgg tttatccggt
    12241 cgcgagcgcc gaaggttgcg agctaatttt gtctgacggc agacgcctgg ttgacggtat
    12301 gtcgtcctgg tgggcggcga tccacggcta taatcacccg cagcttaatg cggcgatgaa
    12361 gtcgcaaatt gatgctatgt cgcatgtgat gtttggcggt atcacccatg cgcccgccat
    12421 cgagctatgc cgcaaactgg tggcaatgac gccgcaaccg ctggaatgcg tttttctcgc
    12481 ggactccggc tccgtagcgg tggaagtggc gatgaaaatg gcgttgcagt actggcaggc
    12541 caaaggcgaa gcgcgccagc gttttctgac cttccgcaat ggttatcatg gcgatacctt
    12601 tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac agcctgtgga aaggctatct
    12661 gccagaaaac ttgtttgctc ccgccccgca aagccgcatg gatggtgaat gggatgaacg
    12721 tgatatggtt ggctttgccc gcctgatggc ggcgcatcgt catgaaatcg cggcggtgat
    12781 cattgaaccg attgtccagg gcgcaggcgg gatgcgcatg taccatccgg aatggttaaa
    12841 acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg attgccgacg agatcgccac
    12901 cggatttggt cgtaccggca aactgtttgc ctgtgaacat gcagaaatcg cgccggacat
    12961 tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc ctttccgcca cgctcaccac
    13021 gcgcgaggtt gcggaaacca tcagtaacgg cgaagccggt tgctttatgc atgggccaac
    13081 ttttatgggc aatccgctgg cctgcgcggc agcaaacgcc agcctggcga ttctcgaatc
    13141 tggcgactgg cagcaacagg tggcggctat tgaagtacag ctgcgcgagc aacttgcccc
    13201 cgcccgtgat gccgaaatgg ttgccgatgt gcgcgtgctg ggggcaatcg gcgtagtcga
    13261 aaccactcat ccggtgaata tggcggcgct gcaaaaattc tttgtcgaac agggtgtctg
    13321 gatccggcct tttggcaaac tgatttacct gatgccgccc tatattattc tcccgcaaca
    13381 gttgcagcgt ctgaccgcag cggttaaccg cgcggtacag gatgaaacat ttttttgcca
    13441 ataacgagaa gtccgcgtga gggtttctgg ctacactttc tgcaaacaag aaaggagggt
    13501 tcatgaaact catcagtaac gatctgcgcg atggcgataa attgccgcat cgtcatgtct
    13561 ttaacggcat gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc
    13621 ctgcgggaac gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca accggctccg
    13681 gctggtggca ctgggtagtt gttaacttac ccgctgatac ccgcgtatta ccgcaagggt
    13741 ttggctctgg tctggtagca atgccagacg gcgttttgca gacgcgtacc gactttggta
    13801 aaaccgggta cgatggcgca gcaccgccga aaggcgaaac tcatcgctac atttttaccg
    13861 ttcacgcgct ggatatagaa cgtattgatg tcgatgaagg tgccagcggc gcgatggtcg
    13921 ggtttaacgt tcatttccac tctctggcaa gcgcctcgat tactgcgatg tttagttaat
    13981 cactctgcca gatggcgcaa tgccatctgg tatcacttaa aggtattaaa aacaactttt
    14041 tgtcttttta ccttcccgtt tcgctcaagt tagtataaaa aagcaggctt caacggattc
    14101 atttttctat ttcatagccc ggagcaacct gtgaacacat tttcagtttc ccgtctggcg
    14161 ctggcattgg cttttggcgt gacgctgacc gcctgtagct caaccccgcc cgatcaacgt
    14221 ccttctgatc aaaccgcgcc tggtacctct tctcgcccga ttctgtcggc aaaagaagcg
    14281 cagaatttcg atgctcaaca ctattttgca tccctgacac caggtgctgc agcgtggaat
    14341 ccttccccga ttaccctgcc tgcgcaacct gactttgttg tcggcccggc gggcactcaa
    14401 ggtgtaacgc ataccacgat tcaggcggcg gtagatgcgg caattatcaa gcgtaccaac
    14461 aagcgccagt atattgccgt gatgcctggt gagtatcagg gaacggtata tgtccctgcc
    14521 gctccgggtg gaattactct gtacggtaca ggtgaaaaac cgattgatgt gaagattggg
    14581 ctttcccttg atggtggcat gagccctgcc gactggcgtc acgacgtcaa cccgcgcggc
    14641 aaatatatgc caggtaaacc agcgtggtat atgtacgata gctgccagag caaacgcagc
    14701 gacagtatcg gtgttctctg ctctgcggtc ttctggtcac aaaacaatgg cctgcaactg
    14761 caaaatctga ccatcgaaaa cacgctgggc gatagcgtag atgcaggtaa ccatccggcg
    14821 gtggcactgc gtactgatgg tgaccaggta cagattaaca acgttaacat tctcggtcgt
    14881 cagaacacct tctttgtcac caacagcggt gtgcagaacc gtctggaaac caaccgtcag
    14941 ccgcgtacgc tggtgaccaa cagctacatt gaaggggatg tggatatcgt ttctggtcgc
    15001 ggcgcagtgg tgttcgataa caccgaattc cgcgtggtga actcacgtac tcagcaagaa
    15061 gcgtatgtgt ttgcaccggc tacgctgtcc aacatttact acggtttcct cgccgtaaac
    15121 agccgtttca atgctttcgg tgatggtgtg gcgcaactgg gccgctcgct ggatgttgat
    15181 gccaatacca acggtcaggt ggtgatccgt gatagcgcca tcaacgaagg ttttaacacg
    15241 gctaaaccgt gggccgatgc ggtgatctct aatcgtccgt ttgcgggtaa taccggcagc
    15301 gtagatgata acgacgaaat acagcgcaat ctgaatgaca ctaactacaa ccgcatgtgg
    15361 gaatacaata accgcggcgt gggtagtaaa gtggttgcag aggcgaagaa gtaagagcaa
    15421 ttaactattt gccggatgcg gcgtaaacgc cttatccggc ctacggttcg atgcgatttg
    15481 taggtcggat aagatgcgca agcatcgcat ccgacaataa gtgccggatg ctgcgaaaat
    15541 gccttatctg gcctacagat tcgatacgat tcgtaggtcg gataagatgc gcaagcatcg
    15601 catccgacaa taagtgccga atgcgaccta cattcacatg gcgcttttta catctgacgg
    15661 tttttattga agttaatcaa actacccgcc ttgataatct cgcgctcttc agcagtcaga
    15721 ctttccatat agagcgtaat ttccgttacc ggcgcatctt catggatcac ataaccttta
    15781 aacgtcgtac ccggattatc cagcgccgct ttaatgccag ggatgtaaat gtaatccccc
    15841 acttcaaagg ttggcacttc cgccatctgc agcggtaaca tcccccagtt gatgacgtta
    15901 gaacggtagc gtttagtcgc gtactcttcg gcaatattcg ccagaccgcc aatcacacgc
    15961 tggcagctcg ccgcctgttc acgcgcagaa ccatcgcctg gtttcaccgc atataccatg
    16021 ctgccaattt cagtttgcag cggatcaata tgctcctgac cagcaatctg cttaatgcgc
    16081 gcaaacacct ctgtcagctc gctgacattc cccgccagac gctgattttc cagctcagca
    16141 gtcgctttac ttctgccaac ataaccggga tcgcggcgtg acagggtaaa ctccgccaga
    16201 ccaatcggat ttgaacgata agaagaggtt tcaccggaag gaatcagttc gtcggtggtg
    16261 gtcacttcgt cgaggatctt cgagcacact ttcaggacga tattgtcagt cagcgcaccc
    16321 aattccggcc agtctttaat gttcggtccg taaatcagcg gttgctgagt tgcccctttc
    16381 acaaagccct gataaacacg gtttttatac ggcgttacat cgaaggcgta ctccggcacg
    16441 ttgtcccagc aatcaagttc gctggcagag gttaaatagc caccgtttgc cgcagtcgca
    16501 gcgatagaac gagcgtccat caacgccacc gctgacatct gcccattagc tggcttagag
    16561 ccttcgcggt tcgggaagtt acgcgtggtg tggcgaatac tcaaaccgtt gttgattggc
    16621 gtatcgcccg cgccaaagca tgggccgcag aacgcggttc tgatgattgc gcctgcgcca
    16681 atcaaatctg ctaccacacc ttttttggcg agatccataa acaccggctg tgatgacggg
    16741 taaactgcca gcgagaaggt gtcattgcca caggattgac cgcgcagtgc attcgccgcc
    16801 gcgatgacgt tttcgtagtt accgccagaa cagcccgcga taatcccctg ctgcactttc
    16861 aggcgaccat tttccacttt atccagcagc gagagtttgg ctttaccgtg cgccacgcgt
    16921 tcggactcaa tttcaatctc acgcagaatg tcggtcaggt tctggttcag tgtgtcgatt
    16981 ttatacacgt tgctcgggtg gaacggcagc gcaatcattg gtttgatggc gcttaaatca
    17041 acgctgatgc agccatcgta gtacgccatc ggttgagggt taagctggca gtaatcctgg
    17101 ccgcgaccgt gcagcgccag ccagttatgc acttcttcat cagtttgcca gacggagctt
    17161 aaacaggtgg tttccgtggt catcacgtca acgctgttac ggaaatcggt agagagcgcg
    17221 ctaacgcccg gtccaacgaa ctccatgact ttgtttttga cgtaaccgtt tttgaacacc
    17281 gcgctaatga tagccagcgc cacatcctgt ggccccacat acggcgcagg ttttccggtc
    17341 agatgcaccg caaccacgcc cggatagtcg atatcccagg tgtcattaag cagctgtttt
    17401 accaactcac cgccaccctc accgactgcc attgtcccta atgcaccgta acgggtgtgg
    17461 ctgtctgacc cgaggatcat tttgccgccg cctgccatca tctcacgcat atattgatgg
    17521 atgaccgcaa tgtgcggagg cacaaaaata ccgccataac gctgggccgc cgataaacca
    17581 aaaacatggt catcaccgtt aatggtgccg ccgacggcgc agagtgagtt atggcagttg
    17641 gtcagcacat acggcagcgg gaaacgttcc attccggacg ctttagcggt ctgtacaata
    17701 ccaacaaagg taatatcgtg agaggctaat gaatcaaact taattttaag tttatccata
    17761 tttccggacg tattatgaga agagagaata gaccaggcaa tagtgccttt tttggcttct
    17821 tcttttttaa tttcgccggt gaaatgttct tcggcaatta tttcgttatt actggcgaga
    17881 aacacgcctt tttcagataa cttgatcatc aaatgctcca gatatctgcg caccgtgaag
    17941 tgcgcagaaa gataataaat ggatatactc taaataattc gagttgcagg aaggcgacaa
    18001 gtgagtgaat ccccaggagc ttacataagt aagtgactgg ggtgagcgaa cgcaaacgca
    18061 gcacatgcaa cttgaagtat gacgagtata tttacagcat gttccagccg atcagcatat
    18121 tccaccacca cacgccgagg gtgatatgca ccaggaaggt taatatcgtc agtaccgcac
    18181 cgaccaacca ccaggattta atatcgttgt aacccacgcc aaagatgacc ggacctgccg
    18241 cgccgccata gtgagtaacc atgccgccat aggagttgga gaacaacagt gccagcgcgg
    18301 ttaacattaa cggtgcgccg gagacgttcg ccagcatggc aaataccggt aacatagcaa
    18361 cgatataggc actaccggaa gcgaagaaat aacgcacgat aatgctgagg aaaataataa
    18421 cgaagaaagc aacgttaccg tgaccatcaa atgccaggtt atttttaaag acttcagcta
    18481 accattcgaa gaatttaact ttcgataata aggagcttaa tccgataata ccgccgtacc
    18541 agattaaggt attccagccg cctttatttt taaccacgtc ttcccaggta acgataccca
    18601 gcagcagcat ggtcgccata acaacgattg ccacggtgga ttcatcaacc cccagagact
    18661 tactgaaaat ccagcccagc agcgccagca caaagacacc gagcagcatt ttttcgcgga
    18721 ttttcatcgg tcccagttcg gcaaggcccg ctttagcgat ggttttatta tccaccttct
    18781 taatttctgg cggatacatg gtataaatca ccagcggggt gaccagcagc ataatgatgc
    18841 ccggcaatcc ggcggccagt gcccatccac cccagctaat ttgcaggtgc agaatgtcgt
    18901 tgatcatttt cagcgccaga atgttccccg ccattgcggt aaagaacata tagctggtgg
    18961 ttttggtgac catgtaaatg gacatcatca ggtaatgtcc gacacgacgc ggactttttt
    19021 ccggttcgga ccccaaagcc accgccacgc tgttgatgat cggtaacaca atgccgcccg
    19081 cacgcgcggt gttagacggt gttgccggag ccagtaccag atcgaggaat accgtaacgt
    19141 aacccagacc cagcgtggtg ttaccgattt taccaatcag cagataggca atacgtttac
    19201 ctaaaccggt ggtcacaaat gcggcgctta aggtaaacgc cgagaacacc agccaggtag
    19261 tacctgaaga gtaaccgctt aatacggcgg tggtcttaaa cgcaccgtcg gataagttac
    19321 cgaccaccac catggaggca gcaacggcaa ttaacagtac gacaggttcc gggaaaggct
    19381 tgataaccag ccccacaatg gccgccaggt aaataccaaa aagcacccac gccagttcgc
    19441 ttaatcctgc cggagctggc ataaaaccaa taatacatgg gatcgctaat atcagaatta
    19501 gcttccataa cgatttctta ttcatttaaa atcctgttct ttctgtatga ctcatacaga
    19561 agtcctttat tgattgtcct tatcctgaca acgaattttt tcagggaaga taaacttcac
    19621 cggaaaatat ttttctggtc gtccgaataa cagatgcgcg taacgtcgtg gcatcctgac
    19681 cttcattact taaatgaacg tcgagcgcac cactggggtg ttcaatattg atattgccgt
    19741 atcctacaga agggacgatt tgtcgggtga cggtgccttc caatgcacaa ctactggaaa
    19801 tagcaatagc accggttatc gccagcgcgc gatggcaaga atgcggcata aaataacgca
    19861 cattaattgc cccgcctttc tgcgctggag aaataagcac aggtttaggg ataaccatat
    19921 tactgacatc acctaagccc attgctttac ccgcttgtag acggatagat tcaatgcggg
    19981 ctaataatgc tttgtcggca tccagttccg ccggtaattc ataacctgtt tttcccagat
    20041 attcagccgg aataatgacg actggcatcg ccatatcgat acaggtcacc gggacatcgt
    20101 caaaataatc aatctgatta tcagtcggga agacttttcc ggttttggtt ccggcggcat
    20161 tcaggaaagt gagcgcaacc ggtgcggcag tacccggtac gccgtcaatt ctggcgctac
    20221 cctcgtactc gacaacacca tttggcgttt gcacatcagc ttcgatgaac gtaccagtat
    20281 tgacgttgcg gatacgtacg cgggtaactg gtgaagtcgc tgcaatcaaa ccattttcaa
    20341 tggcgaatgc cccaacacca gacagcatat tgccacaatt aggcgtggta tcgacacgtt
    20401 gctcatggac aataacttga gcaaacagat aatcgacatc agcacgcgga tcgctggaac
    20461 ggctaataat ggcgacttta ctggtcagcg gattaccgcc gccaataccg tcaatttcca
    20521 gatcgttacc ggaacccata attgccatca atattttatc gcgctgcgtt tgatcttcgg
    20581 gtaaatgttc cgctaacaag aacgcgcccc tcgaggttcc acctcgcatc atcacgcagg
    20641 gtattttttt cataataatg ccctcaattt aaaaacataa cttattgcga tatggttaca
    20701 ttaaggacaa agcatctcta atgactttag ctcaacgatt aataacattt tttcattaat
    20761 ttaagttggc taaatgcagt taaaattttt aacgaccagc cacccaaaat cgtcgcttcc
    20821 attttccgga gtaataatga agcatgaatt atcaagtatg aaggcatttg tcatactggc
    20881 agagtccagt tcatttaaca atgccgctaa attactcaat attacgcaac ctgcattaac
    20941 gcgaagaata aaaaagatgg aggaagattt acatattcaa ctatttgagc gtacaactcg
    21001 caaggttacg ttaacaaaag caggaaaaag gttgctcccg gaggcgcggg aattaataaa
    21061 gaaattcgat gaaacgcttt ttaatattcg tgatatgaat gcttatcatc gtggtatggt
    21121 gacgttagcg tgtattccga ccgcagtatt ttatttttta ccgctggcaa tcggtaaatt
    21181 taatgagctg tatcccaata ttaaagtgcg gattctggaa caaggcacga ataattgcat
    21241 ggaatcggtg ctgtgtaacg aatcagactt tggcataaac atgaacaacg tcacaaattc
    21301 atccattgat tttaccccac tggtcaacga gccattcgtg ctggcctgtc ggcgtgacca
    21361 tccattggcc aaaaagcagc ttgtagaatg gcaggagtta gtgggttaca aaatgattgg
    21421 cgtacgttcc tccagcggca accgattgtt gatagaacaa cagctagccg acaagccctg
    21481 gaagctggac tggttttacg aagtgcgtca tctttcgacg tcattaggac tagtggaggc
    21541 aggactgggg atttcggcgc ttcctggtct ggcaatgccc catgcgccct attcttcgat
    21601 tattggtatc ccactggtgg aacccgttat acgtcggaca ttggggatta ttcgccgcaa
    21661 agatgccgtg ctttctccgg cagctgaacg cttttttgcc ttgctgatta atttgtggac
    21721 tgacgataaa gacaatttgt ggaccaatat cgtcgaacgc cagagacatg cgttacagga
    21781 gataggttaa tacaccggcg caggagagac tgcgccggtt aaatcagcgg ttagtgtgcg
    21841 ttaaccacca cccacattgg tccctgcccg accgcataac ggcctttttc atgcagtagt
    21901 ccctgctcgc caacaatttc gtataccgag atgtggtgag atttttgccc ggcggcaatc
    21961 agatacttgc cgctgtgatc aacattgaag ccgcgcggct gggtttccgt tggctggaag
    22021 ccttctttac tcaacacgct gccatcttcc gaaacgctga aaacggtaat caggctggcg
    22081 gtacggtcgc aggcgtataa atggcgacca tccggggtga tatgaatatc agccgcccaa
    22141 cgggtgtcgg agaagttttc cggcatcata tccagcgtct ggacacattc gatattaccg
    22201 tgcggatctt tcagttccca gacatccact gagctgttta actcattgac gcaatacgca
    22261 tattgttcgt ttggatggaa taccatatga cgcgggccgg ccccttcaac ggtggtcact
    22321 tccgcagggt cctgcgccac gagatgacca tcatcgctga ccgtaaacag gcaaatgcga
    22381 tcctgcttta atgccggaac ccacagcgta cggttgtccg gtgagatatt ggcggaatgg
    22441 caaccgtcca gcccctcgac cacatcgacg acgcccactg gcaggccatc ttccagacgc
    22501 gttacgctca cgttacccgc attgtaagaa cctacaaaga caaactgccc ctggtgatcg
    22561 gtggaaatat gcgtcggact acccggcagc gcagactctg cggcaaaggt cagtgcgcca
    22621 tcgtccgggg cgatacgata cgccaggacg cgaaactcag ggcgaacacc aacatagaga
    22681 taacgtttgt ccgggctgac caccatcggc tgcacctgcc ccggcacatc gacaacctgt
    22741 gtcagcgtca gtgcgccttc atgattcagg ttccagacgt gaatttgctg gctctcaggg
    22801 ctggcgatat aaactgtttg cttcatgaat gctcctttgc atttagcaac tgactgtaac
    22861 agctaaaatt agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat
    22921 agtggtatcg gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc
    22981 atgaccacac gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc
    23041 ctgcttcctt catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc
    23101 atcgtcacag gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat
    23161 acacctgcta tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg
    23221 gaagcggacc caatgcccgt taataaagcc ctgcaactca ttgagatgct gaatgaacac
    23281 cacattcacg gtctgatgta tgtggatgat gcaatggtct atgagcaccc gaccgggcat
    23341 gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca
    23401 caagtcgctt ctctggctga aacggcgcaa caagttaacg ccgtatggaa gttcgccctg
    23461 acgcacgatg acctgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga
    23521 ctggagtgtg aatggtcctg gcacgatcag gttgatattg cacgcggcgg caacagcaaa
    23581 ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa cgtcgtggcg
    23641 ttcggcgata actttaatga tatcagtatg ctggaggccg ctggtacagg cgtggcgatg
    23701 ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
    23761 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
    23821 tttaatttgc gcgtacagcc acaggccagg tttgatcacc agttcatccc tggcccacgg
    23881 gctgatacgc gcccacagcg ttttacctcc gacttccagt tccacttcca cctggccgtt
    23941 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
    24001 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
    24061 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cagtcatcgc
    24121 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
    24181 cagccacgga ttcatcacgc tactacccca gacttcttcc agtgcgccaa aggctttcac
    24241 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
    24301 gctgacatac aacatcggga tgttgatttc ccgcgtcaga cgttgcagat aaggcaacag
    24361 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattctgg
    24421 tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg acaggctgcc
    24481 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
    24541 gaccatgctt ttcgccatgc cgtagcgcag attgccacgc actttgtaat gcggaaacag
    24601 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttct ggcgtcaggc agataccttt
    24661 ttcggcatca tttagtaccc gaccattgag gacaatcctc cctttttgcg ggcgcgtcag
    24721 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
    24781 gatgccattg gcgggcagcg tttcattaat ggtcaggcaa tggttgccca aggtctggga
    24841 aaaattcagt tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
    24901 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
    24961 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagaaggaat ggttcgcgtt
    25021 tcaccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
    25081 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
    25141 aaaacgcgcc agcgcccggc ccccagtgtt cttgcggcct gttcgagttt gacatcaacc
    25201 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
    25261 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
    25321 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
    25381 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
    25441 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
    25501 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
    25561 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
    25621 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
    25681 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
    25741 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
    25801 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
    25861 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgccagg cgaccgccat
    25921 tcagcagcga agtccagttg gttttgctgt cgatgatgaa atctttctgc tcgctggctt
    25981 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
    26041 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
    26101 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
    26161 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
    26221 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
    26281 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
    26341 aacgttatgc aggcgacaat ataacggcta gctcagaatt ttcccagcag ttattgctaa
    26401 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
    26461 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
    26521 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
    26581 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
    26641 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
    26701 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
    26761 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
    26821 ccgcgccgca tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
    26881 aaagatgccg gtattagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
    26941 agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
    27001 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
    27061 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
    27121 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
    27181 gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgatggaaa aacacgcctg
    27241 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
    27301 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
    27361 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
    27421 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
    27481 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
    27541 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
    27601 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
    27661 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
    27721 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
    27781 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
    27841 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
    27901 tggcgaagat gacaccggac gcactacggc tgagatcatt caggatgaag taaaggatgc
    27961 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
    28021 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
    28081 gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct cacgtcagca
    28141 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
    28201 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
    28261 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
    28321 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
    28381 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
    28441 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
    28501 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
    28561 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
    28621 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
    28681 taccgtgcgt aatgtgattc tttctggcta tttcgattcg attggcattt atcaagccgt
    28741 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
    28801 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
    28861 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
    28921 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
    28981 gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
    29041 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
    29101 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
    29161 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
    29221 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
    29281 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
    29341 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
    29401 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
    29461 agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt
    29521 gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca cgctatcgac
    29581 accgtgatcc acttcgccgg gctgaaagcc gttggcgaat cggtacaaaa accgctggaa
    29641 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
    29701 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
    29761 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
    29821 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
    29881 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
    29941 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
    30001 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
    30061 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
    30121 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
    30181 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
    30241 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
    30301 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
    30361 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
    30421 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
    30481 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
    30541 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac caggacttac
    30601 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
    30661 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
    30721 aatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
    30781 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
    30841 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
    30901 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
    30961 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
    31021 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
    31081 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
    31141 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
    31201 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
    31261 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
    31321 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
    31381 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
    31441 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
    31501 ctggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
    31561 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg cgccacgcga tgaccgtaaa
    31621 gttcgcgtaa tggcagccga ttatgaaaat cagctcgacg agttttccct cgatgcgccc
    31681 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
    31741 caactgcgta acaacagctt cggcggcgtg gacatggtga tcagcggcaa tgtgccgcag
    31801 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
    31861 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
    31921 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
    31981 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
    32041 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
    32101 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
    32161 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
    32221 cgcgtgcggc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
    32281 caaggcgacc tgaaacgtat gggcgagttg atggcggaat ctcatgcctc tatgcgcgat
    32341 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agctgtgatt
    32401 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
    32461 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
    32521 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
    32581 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
    32641 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
    32701 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
    32761 aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
    32821 gccgttatac ctttgacggt gaaaccgtga cgctttcgcc aagtcagggc gttaaccagc
    32881 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
    32941 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
    33001 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctctattact tatcgcgcca
    33061 cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    33121 agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat ctgccggttg
    33181 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacgtct tttgatttcc
    33241 gtagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    33301 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    33361 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg gctctgcaat
    33421 tctactccgg caacttcctc ggcggcacgc catcaagagg aactgaacct tacgccgact
    33481 ggcaaggcct ggcactggaa agcgagtttc tgccggacag cccaaaccac cctgaatggc
    33541 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    33601 ttattgctga gtagcacctg aagccctcgt aatgagggct ttatccttat attgctcaaa
    33661 tcaccagcaa acaccgacat atttgcaact caatattcac aacaacctta cactgcgaca
    33721 ctattttcgc tatggttatg cgtaagcatt gctgttgctt cgtcacggca atataatgag
    33781 aattattatc attaaaaaat gatttgagga gtaagtatat ggctgtaact aagctggttc
    33841 tggttcgtca tggcgaaagt cagtggaaca aagaaaaccg tttcaccggt tggtacgacg
    33901 tggatctgtc tgagaaaggc gtaagcgaag caaaagcagc aggtaagctg ctgaaagagg
    33961 aaggttacag ctttgacttt gcttacacct ctgtgctgaa acgcgctatc cataccctgt
    34021 ggaatgtgct ggacgaactg gatcaggcat ggctgcccgt tgagaaatcc tggaaactga
    34081 acgaacgtca ctacggtgcg ttgcagggtc tgaacaaagc ggaaactgct gaaaagtatg
    34141 gcgacgagca ggtgaaacag tggcgtcgtg gttttgcagt gactccgccg gaactgacta
    34201 aagatgatga gcgttatccg ggtcacgatc cgcgttacgc gaaactgagc gaaaaagaac
    34261 tgccgctgac ggaaagcctg gcgctgacca ttgaccgcgt gatcccttac tggaatgaaa
    34321 ctattctgcc gcgtatgaag agcggtgagc gcgtgatcat cgctgcacac ggtaactctt
    34381 tacgtgcgct ggtgaaatat cttgataaca tgagcgaaga agagattctt gagcttaata
    34441 tcccgactgg cgtgccgctg gtgtatgagt tcgacgagaa tttcaaaccg ctgaaacgct
    34501 attatctggg taatgctgac gagatcgcag cgaaagcagc ggcggttgca aaccagggta
    34561 aagcgaagta aacgtcattc gtttaaaatg agaaagccga ctgcaagtga gtcggcattt
    34621 ttgttgctaa caatggagca cattgcctga tgcgacgctg cgcgacttat caggcctgtg
    34681 gtgattcatc ggatacgcca ctctgacggc gcatccgaca attaaacctt acccgcgacg
    34741 cgcttttact gcattcgcca gttgacgtaa cagagcatcg gtatcttccc agccgatgca
    34801 ggcatcggtg atgctcttac cgtaggccag cggctccccg ctctcgaggc tctgattgcc
    34861 ttccaccaga tggctttcca ccatcacgcc aataatggcc ttttcgccac cggcaatctg
    34921 ctggcaaacg tcagcacaaa catccatctg ctttttgaat tgtttggacg agttagcatg
    34981 gctgaaatcg atcatcacct gtgctggcag gcctgctttg ttcagccctt ctttcacttc
    35041 agcaacgtgc ttcgcgctgt agttaggctc tttaccgccg cgcagaatga tatggcaatc
    35101 gccgttaccg ctggtattca caatcgccga atgcccccat ttcgttacgg acaggaagca
    35161 gtgcggcgca ccggcggcat taatggcatc gatagccact ttaatcgtac cgtcggtgcc
    35221 atttttgaag ccgaccggac aagaaagccc tgatgccagt tcgcggtgca cctgcgattc
    35281 ggtggtacgt gcgccaattg cgccccagct catcaggtca gcgagatatt gtggggtgat
    35341 catatcgaga aactcacctg ccgctggcag accgctgtcg ttaatatcaa gcagcaattt
    35401 acgggctata cgcagaccgt cgttgatctg gaagctatta tccatatgcg gatcgttaat
    35461 cagccctttc cagcccaccg tggtacgcgg cttttcaaaa tagacgcgca ttacgatttc
    35521 cagctcatct ttcagctctt cacgcagcgc cagcaagcga gtggcatact cttttgccgc
    35581 gacaggagca tgaattgagc atgggccaat cacaaccaac aggcgatcat cattaccttt
    35641 caggatctta tggatcgctt ttcgggcatg ggcaaccgta ttcgcggcat tttcagtagc
    35701 ggggaatttt tccagcaatg cgacaggagg aagtaactct ttgatttctt tgatgcgtaa
    35761 atcgtcgttc tgataattca tgtctgttcc agtgttgcca tacttatctt agtgaatgca
    35821 atacttccaa tctatatctt ccgtcagaat gtgtaaacgg ggttttacac tatgaacgga
    35881 ttaatcctgg aattagcaag aaaaacgcca gattgtcgcg aaaaacgaga tctctcctac
    35941 aatttctaac tgtaactcct ttgtttgtta attatttcaa gattctctgc tgcgtttcat
    36001 aacctggctg aaatcttaaa ccaatgcctt atattcacct gcaaatgcac tgttggaaga
    36061 ggttatccga cataacgacc ataacaggag catcctatga aaatgacaaa actggccaca
    36121 ttatttctga ctgccactct aagccttgcc agcggtgccg cactggctgc cgatagcgga
    36181 gcgcaaacta ataacggcca ggcaaacgcc gcagctgatg cgggccaggt agcccctgac
    36241 gcccgtgaaa atgtcgcgcc aaataacgtc gacaataacg gggtaaatac cggttctggt
    36301 ggcacaatgc tgcattcgga tggttcttca atgaacaatg acggaatgac caaagatgaa
    36361 gagcacaaga acaccatgtg caaagatggt cgctgtccgg atatcaataa aaaggtacaa
    36421 accggtgatg gcatcaataa tgatgtcgat accaaaaccg acggtaccac gcagtaaccc
    36481 tgcaaaatag ccaataatgg gagagcggag tctctccctt ttaatttttc acttctaaaa
    36541 gcagtatgat gtcataaagt taagtgagaa taattaaggg atgactcgtc atggcgcact
    36601 cacactcaca cacgtcctca cacctgccag aagataataa tgctcgtcgc ttgttgtatg
    36661 ctttcggcgt gactgccggg tttatgctgg tagaagtcgt tggtggtttt ctttctggtt
    36721 ctctggcatt gctggccgat gcgggtcata tgttgaccga tactgccgcc ctgcttttcg
    36781 ccctactcgc cgtgcaattt tcacgtcgtc ctcccaccat tcgccacact ttcggttggc
    36841 taagactgac cacgctcgcg gcgtttgtga atgccatagc cctcgtggta attaccattt
    36901 tgattgtctg ggaggcgata gaacgcttcc gcacgccgcg tccggtcgag ggcggcatga
    36961 tgatggcaat tgccgtggct gggttgctgg caaatatact ttctttctgg ttacttcatc
    37021 acggcagcga agagaaaaac ctcaacgtac gagcagcggc actgcatgtg ctcggggacc
    37081 tgctgggttc ggttggggct attattgccg cgttgattat tatctggact ggctggacac
    37141 ctgctgaccc cattctctcg atactggtgt cgcttctggt tctgcgtagc gcgtggcgac
    37201 tgttgaaaga tagcgtgaat gaattacttg aaggtgcacc ggtatcgctg gatatcgctg
    37261 aactgaagcg tcgtatgtgc cgggaaatcc cggaagtccg caatgtgcac catgtacatg
    37321 tatggatggt aggcgagaag ccggtgatga cgttgcatgt gcaggtgatc ccgccacacg
    37381 atcacgatgc cttgttggat cagatccaac actacctgat ggatcactat cagattgagc
    37441 acgccaccat tcagatggaa tatcaacctt gtcatgggcc agactgccac ctgaatgaag
    37501 gggtgtctgg ccattcacat catcaccatt aatgggacag cgcgcgtgag cctctttcac
    37561 gtgcgctgtt gatccacatc cggctgccgt tgagcgcaat aaaggtcagg atgatgtact
    37621 ccagagacat ggcgtaaacg ccctgaagtg caaaaataac gacgctaatc acgttaataa
    37681 tcacccacaa cagccagttt tccacatact tacgcgtcat cagaatcatt gccacgatag
    37741 ataacaccat catgcatgaa tcccagaacg ggaaagcgtc cggttgcagt tcaggcatca
    37801 caacctgtaa tcctaatgct tgcatgatca tgactgccac gcgggtcaaa aatgcaaaca
    37861 ccggattgat aaataccgtc atcagaccaa tcgaaacaac gcaaaccgcc aaccagctga
    37921 gtgccttcgg caatggcaac cagcgaattt tcaactccgc ctcgttctga ctggtttgtc
    37981 gcgaccacgc ataccaaccg taaatattcg cggcaaagaa aaacacctgt aatagcaggc
    38041 tggcatacag ctgaatctga aagaaaataa tgccaaacaa ggtgacgttg atcaggccaa
    38101 agaagtagtt gctgatcttc tccagactgg caaggccaat acacagcaac ccggcgatcg
    38161 tgcctaccgc ttcgatccat gagagatcat aaccgcctgc ccctattggt atatgtacca
    38221 ggatattctg cacactaaaa aaatccatct ttcccccaaa gcgtaacgtt acgccttatt
    38281 attcgttatc cacgtagtgt agccgcaaaa tccagcatac gattgagcgg caccagcgcc
    38341 ctctctcgca gcctttcatc aacatgaacc tcgtgattgc ttccttcctg ttctaatgcc
    38401 tctgcgatgg actgaaggcc attcatggcc atccacggac aatgcgcgca gctgcggcag
    38461 gttgcaccct cacctgcggt tggtgcttcc agtaactctt tatctggcac tgcctgctgc
    38521 attttgtaga aaatgccccg atcggttgcc acgataagcc gctgatgtgg caatgttttc
    38581 gcagcagcga tcagttgact ggtggaaccg accgcatccg ccatatcgac aatagcttgt
    38641 ggtgattctg gatgcaccag tatggcagca tccgggtatt cttcttgcaa gcgggttaac
    38701 gcctgagtct taaattcatc atgcacaata caggcaccct gccagcatag aatgtctcca
    38761 cccgtctgtt tttgcacgta acgccccaga tgtttgtcgg gtgcccagat gattttttca
    38821 cccaaactat caagatgatc aataagttcg acggcaatgc ttgaagttac cacccaatct
    38881 gcgcgcgctt ttaccgcagc agaagtgttg gcgtagacga cgacagtacg atcgggatgg
    38941 gcatcgcaaa atgcgttaaa ttcttcaaca gggcagccga gatccagtga acattcagcc
    39001 tgaagtgtcg gcatcagaat tgttttttcc ggactgagaa ttttggcggt ttctcccata
    39061 aatctcaccc cagcgactaa caaagtagaa gcgggatgct ttgcaccgaa gcgcgccatt
    39121 tccagagaat cagaaataca gccaccggtt tcttctgcca gttgttgaat ttcgggatcg
    39181 gtatagtagt gggcaaccat caccgcatta cgttctttta gcagacgttt tatcttctcg
    39241 cggtaatacg ctttttcatc aatgcttaac ggcgtcggct tcggggggaa aggataaatc
    39301 gccgtgtctg gatcaaacat tacgctcatc ttaccatctc gttttacagg cttaacgtta
    39361 aaaccgacat tagcgtaata ttcgctgtta tcggtgtaat ttgttttata tgctaaacaa
    39421 gatagtcgaa tggtggggaa aagtcactcg aattacgcct aattccaccg acgtagagtt
    39481 gcttgcacag taagctctga cgatgtcatc aggtttacaa agtgaaattg aggatgcctg
    39541 aatagcaaaa aagcgccttt agggcgcttt tttacattgg tgggtcgtgc aggattcgaa
    39601 cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga ccccttgcgg
    39661 gattatgctg ctttaatcat tcaggatggt gggtcgtgca ggattcgaac ctgcgaccaa
    39721 ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccatacgggt gttacctgaa
    39781 agatttctac tcggggccaa tctttaagat tggtgtgtcg tgctggatga ctcgcttcgc
    39841 ttgccctacg ggccgttgct ggcgcaacgt tatccttcac gcttaacatc tgagtttgat
    39901 gttaaaattg gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg
    39961 ctctaccaac tgagctaacg acccgagtgg tgggtgatga cgggatcgaa ccgccgaccc
    40021 cctccttgta agggaggtgc tctcccagct gagctaatca cccgatactg cgctggatac
    40081 tacaaactac ttccaccgaa cccaccgtaa ttagtggtgg gtcgtgcagg atgactcgct
    40141 tcgctcgccc tacgggccgt tgctgacgca acgttatcct acacgcttaa catcttgtct
    40201 tcgatgctaa aactggtggg tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc
    40261 aactgctcta ccaactgagc taacgaccct gtggtgggtg atgacgggat cgaaccgccg
    40321 accccctcct tgtaagggag gtgctctccc agctgagcta atcacccaat actacgctgg
    40381 atactacaac taactatgtt agtggtgggt cgtgcaggat gactcgactt cgtctcgccc
    40441 ttcgggccgt tgctgatgca acgttatcct tcacgtttta ctaccgcttc caccttagcg
    40501 attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac
    40561 caactgagct aacgacccac tttttcgttg ctttcggttt gtttgatatc ccgtggcaac
    40621 ggcggcatat attactgatt tcagacttga gcgcaacaaa aatttcgatg cagatcactc
    40681 aactgcttat gattcgcacg acacgaccag aaataatgcg acttctggtc gtgtgttatg
    40741 cattacatcg cgttcagacg tttttgtgcc tgtttagcgc catcagtacc agggtattta
    40801 ctgataacct gctggtatac ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg
    40861 acgccgactt taaacatcgc atcagcagcc tttggtgact tcggatagtt tttcactacc
    40921 gaagcaaaat agtacgccgc atcatctttt ttacccttgt tgtagtttaa ctgacccagc
    40981 caataattgg cgtttggcag gtaagttgaa tcagggtaat ttttgatgaa attctgaaat
    41041 gccaccattg cgtcatcctg gcgggattta tcctgcacca gcgcaatagc tgcattgtaa
    41101 tccgtgtttg catcgccgct tttcaccggc gcgccagcat tcgcagtacc agcatcagct
    41161 gtcggcgtcg ttgatgccgc cgcaccgctt tgatcgctgc tggttgattg cgccgctgca
    41221 ccaccgctgc tgaggctgtc gatctgcaac aggatctgct tttgccgctc cacgacctga
    41281 ttcagttgat actgattttc ctgaatctga ccacgcaggg aatcaatatc ggattgatta
    41341 tcagaaagtt gttgctggag ttgggttaaa agctggctgt gagcattaga aatacgctca
    41401 agttgagtga cgcggtcttc gaccgagcct gagccgacac tactgattgg tgcctgagca
    41461 aaagcggccc agggggccgc tataccaacc agtaacgaca gactcaatag ttgatgtctg
    41521 aagttactgc tcatgcaatt ctcttagtaa accagtaccg cacgacggtt tttggagtat
    41581 gccgcttcgt catgacccag tactgcaggt ttttctttac cgtaagaaac gatggagatc
    41641 tggtctgcag aaacgccttt accctgcagg tacatcttaa cggcgttcgc acgacgttca
    41701 cccagggaga tgttgtattc cggagtacca cgttcgtccg cgtgaccttc tacggtgact
    41761 ttgtaagacg ggttgctacg caggaagttt gcatgtgcat ccagcatttg agcgaagtca
    41821 gaacggatat cgtacttgtc cagatcgaag taaacgatgt tgttctgctg cagctgttgc
    41881 atttgcagac gagcctgctc ttcggaagac atgttgccgt tgccgccgtt cgcatccata
    41941 ccagtgccgg cacccagcat gccttcgctg ccgtcattgc tggcgttctt gttggaagaa
    42001 catgccgcaa ttgccataac aggcagagca atcatcagcc ctttcagcac tttgttcagt
    42061 tgcatttcaa tgattccttt actattcaat taattattat cacagatacg gcgaccaggc
    42121 agggaatttg acctgtccat cagttgccgg aagacgcgct ttgaaacgcc catctgtaga
    42181 aaccaaattc agcacggatc ccatcccctg agaagagctg tagattacca tagtgccgtt
    42241 aggtgccaga cttggcgttt catccaggaa cgtggacgac agaacttgta cgcctcccgt
    42301 tgccagatct tgtttggcaa tgtgctgctg cccaccattg gagctgacca ttaccataaa
    42361 tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa ccttcccagg taatacgttg
    42421 tggcgcaccg ccgttgatat tcactttata aacctgcgga cgaccggcct ggtcagaagt
    42481 aaatgccagg ttctggctat ccgggaacca ggtcggttcg gtattgttac tgcgaccatc
    42541 agtcacctgg cggatctgac cagaagccaa atccattacg tacaggttca gactaccggt
    42601 tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg
    42661 acgcgggaat gaagccacct gacgtacagc gccatttgcc agcgtctgaa taaccagcgc
    42721 ggaacgaccg ctttcgaagg tcacataagc cagtttagaa ccgtctggtg accacgccgg
    42781 agacatcagc ggctgcggtg aacggtgaac gacaaactgg ttgtaaccgt catagtcaga
    42841 tacgcgcagt tcatacggga actgaccgcc gttggtctga acaacgtagg caatacgagt
    42901 acggaacgca cctttaatgc cggtcagttt ttcaaacact tcatcactgg cggtatgacc
    42961 agcataacgc agccactgct tgttcacttt gtacgagttc tgagcaagta cagtacccgg
    43021 tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa gaaccatccg gattcggagt
    43081 gacctgaccg acaactacag cgtcaatgcc cagtgcggac catgcagctg gttgtacttc
    43141 ctgcgcacta cccggctgct gtggcagacg agcgcgatct aacggattaa atttaccgct
    43201 gttacgcaag tcagcagcaa cgatgccgcc aatatcttca ggtgccgcac caggccccgc
    43261 ccactggaaa ggaacaacac caataggacg accggaatct acaccgctgt cgatcacaat
    43321 gcggacttca gcatgcagaa ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa
    43381 tgcctgcttc atcatatctc ccttatctgg accgatggcc cacgataatt tagcagaatg
    43441 ttaacaaact aaaatacaca aaactaccag aaccccgtgg caaccggtgc ctgatgttga
    43501 ccgtccgaac agtcaacatc gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac
    43561 ttcatatact gcctggcttg gtggtttcgg gatcttcgca agtttagctg ctgccaacgc
    43621 agcctgacaa agtgcgggat cgccaccttc aggtttgata tccagtaaca taccatcggg
    43681 tgccagtttt atgcgcagcg tacaggtttt gcctgcatag gacgatgcgt catagaactt
    43741 actttcgata gcagatttaa tctgcccggc atagttattg atatcggccc ctgatgcgcc
    43801 attgttttta gtattaccac tcccggcagg cgaagcattg ttccctttcg cccctccccc
    43861 cgttttcggt gcattcttac cagagcttag ctcaccgaaa atatcatctg cctctgcggc
    43921 cgcttttgct gcagcggctt tttcagctgc agcttttgcc gctgccgctt ttttgtctgc
    43981 ggcggctttt tctgccgctg ctttcttatc agctgctgcc ttttcagcag ccgctttttc
    44041 tgctgctttt ttgtcggctg cagctttctc tgctgccgct ttcttatcag ctgcagcctt
    44101 ttcagcagcc gctttcttct cagcttctgc tttggctttt tcagcagctt cagttgccgc
    44161 tttctttctt gcttcagctg cagctgcttc tgccgcttcc gctttcttct tcagtgccgc
    44221 agcggctgcc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt ctgcttctgc
    44281 ttttttcttt gcgtctgcag ccgctttctt cgctgcttct tccgcagctt tagcatctgc
    44341 ttcggccttc gctttagcat ctgccgccgc tttcgctgcc gcctcttcag cttgcttctg
    44401 ctttaactcg gcctgttttg cggcttcttc agcctgcttt ttctgctcct gagccgctaa
    44461 ccgctctttc tcaagttgct tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc
    44521 ttcagcagcc tgctgttcct tcatcttgcg ctgttcatca gaacgcttcg cgcttgattc
    44581 ctggctttgc atgcgtttgt actgctcaac taccgcacct gaatcaacca tgacagcgtc
    44641 gatggacgaa ccaccgccgc ctccggctga agcttctata ttctcatcga acgaactcca
    44701 gatcagcgcc gcaaataaga tgacatgcag cactgctgaa ataattatcg cccgcttgag
    44761 cttgtcgttt tgttcggttg cctttgacac tctcggtttc caaaaactgt tcgcctgtta
    44821 cccgctctct ttcaagcaag ggaaacgcag atgtttagat aggctgcgtc attaaaccaa
    44881 ccgatttcac acccgcacta tgtaacaagt tcagtgcttt aattatttca tcgtaaggca
    44941 catcttttgc gccaccgatc agaaagaccg ttttcgggtt ggccttgaaa cggctggaca
    45001 cttccgccac cacctgctct ggtggtaaac gctccaggcg atctttctca accaccacgg
    45061 tgtactgacc aataccagac acttcaacaa tcactggcgg attatcgtta ctgctcaccg
    45121 cctgtgattc agtagcgtct ggcagatcga cctccacgct ctgggtgatg atgggcgctg
    45181 tcgccataaa gatcagcaac agcaccagca gtacgtccag caacggtaca atgttgattt
    45241 cggacttgag atcgcgacga cctcgtccac gcgctctggc catggcttac cccttgttgc
    45301 tctcgctaac ggtaaacgcc tggcggtgca gaatcgcggt aaactcttcc ataaagttgt
    45361 cgtaattcag ttccagtttg tttacgcgct ggttgaggcg gttataagcc attaccgccg
    45421 gaattgcggc gaacagacca attgcagtcg caatcaacgc ttctgcgata ccgggcgcaa
    45481 ccatttgcag tgttgcttgt tttaccgccc cgagggcgat aaaggcgtgc atgatccccc
    45541 agaccgtacc aaacagacca atatacgggc tgatggagcc aaccgtaccg aggaacggaa
    45601 tgtgcgtttc cagattttca agttcacggt tcatggagat acgcatagca cgcgacgccc
    45661 cttccactac ggcttccggc gcatggctat tggcacgatg caggcgcaca aactctttga
    45721 acccgctgta aaagatttgt tccgaaccag tcagattatc ccgtttcccc tggctctctt
    45781 gatagaggcg agagagttcg attccagacc agaatttatc ttcaaacgct tcggcttcgc
    45841 gcgccgctgc gttaagaata cgggtccgct ggataataat ggcccaagat gcgattgaaa
    45901 aaccaatcaa aatcaacatg ataagtttaa ccagaaggct agccttcagg aacaaatcaa
    45961 ggatattcat gtcagtcact gcttaaactc cgcgacaata gacttgggaa gcgcacgagg
    46021 cttcattttg agtgggtcaa cgcaaacaac cagaacctct gcttcattca gcaaagtatt
    46081 ctcggcgttg acaatacgtt gcgtgaaaac caaagaggtg ccacgcattg atgttatttc
    46141 agtctgtatt tcgagcatat cgtcgagccg cgcaggtgcg taatattcca ccgtcatttt
    46201 acgtaccaca aaggcaacgc gttcagccat cagcgcctgc tgactgaagt gatgatgacg
    46261 cagcatctct gtgcgtgctc tttcataaaa agcgacgtaa ctggcgtggt acaccacacc
    46321 accggcatcg gtatcttcat agtagacgcg aaccggccat cgaaacagcg ttgtattcac
    46381 tttacatccc ggtaatgcaa caaaagttag agcttttaaa cgctgctact atacgcgcgc
    46441 gattgaggtt tgggaatgag ggcaagttaa gggagcgtaa aaatttatgg gcctggagcg
    46501 gcccataatt ttttaaagag aattctgatt taaaagaaga agaaaatcag cccgacaatg
    46561 agaacaatat cggcaagcaa tgggcaaaaa atcccttgcc aaagaacctt ctgcggacga
    46621 aagcccacgc cgtgaatcac accagcacag acggcccaca tcagcaataa accatgccag
    46681 atttccagtt cactggtctt cgcggcaaaa cgagatgggt cccaaaacat acatcctgct
    46741 aacagaagcg ccatcacgaa ggaaagcgcc cgcagggggc gcttgtccat taccgcatac
    46801 aaagtcgcga taatcttact catcagatgt cttcttgacc ggctttgcct gattcgacgt
    46861 gttcaagcgc cagcgcggtg attaccccaa acgaacaggc aagaagcgtt cccagaatcc
    46921 atgcgaaata ccacattttt agctccttac ttagtacaga gagtgggtgt tacgttcaat
    46981 atcttctttg gtgatacgac cgaacatttt ccagtaacac caggcggtgt agagcagaat
    47041 gatcggtacc agaaccaccg caacccaggt catgacgtta agcgtcagct ggctggaagt
    47101 tgcatcccac attgtcagac ttgcgttcat catggtgctg gacggcatca caaacgggaa
    47161 cattgcgata ccggctgtca ggatgatgca ggccagcgtc agggaggaga acacaaacgc
    47221 ccacgcggct ttatccatac gtgcagtcag gatggtcagc agcggcagaa ccacacccag
    47281 cgccggaata gcccacagaa ttggcgtgtt gttgaagtta accagccatg cgccagcttc
    47341 acgaaccact tctttattca gtgggttaga ggctgcgtaa tggtccattg tcgatttcac
    47401 gacataacca tcgataccgt acatcaccca tacgccagcc agtgcgaaac agaccagtgt
    47461 caccagcgca gccacctgag ccgttgcacg ggtacgcagg tgcagttcgc ccacggtacg
    47521 catttgcaga taggttgcgc cctgagtaat gatcatccct acgctcacca cgcctgccag
    47581 caggccgaac gggttaagca actggaagaa gttaccggtg tagtacagac gcagatattc
    47641 atcaacgttg aacggtacgc cctgcaacag gttaccgaac gctacaccaa ttaccagcgg
    47701 cggaacgaag ctaccaatga agatgcccca gtcccacatg ttacgccagc gggtttcttc
    47761 aatcttggag cggtagtcaa aaccgaccgg acggaagaac aaagacgcca gcacgaggat
    47821 catcgccaca tagaagccgg agaacgcagc ggcatagacc atcggccagg cagcaaagag
    47881 tgcgccgccc gcggtgatca gccaaacctg gttaccgtcc cagtgtggtg caatggagtt
    47941 aatcataatt cgacgctcgg tgtcgttacg accgaggaaa cgggtgagca tgcccacccc
    48001 catgtcgaaa ccgtcagtga ctgcaaaacc aatcagcaga acgccaacca gcagccacca
    48061 gataaaacgc aatacttcat aatcgatcat ttgacgactc ctgtcttagc gtgccggctg
    48121 agtagtcgtg gaagactgct caaagtgata gcgaccggtt ttcaggctgc ttgggccgag
    48181 gcgtgcaaat ttgaacatta agaacaattc tgccaccagg aacagggtat acaggccgca
    48241 aatcagcacc attgagaaga tgagatcgcc tgcggtcagt gacgagttcg ccacagctgt
    48301 cggcagcact tcaccgatag cccacggttg gcggccatat tcagccacga accagcccgc
    48361 ttctacagca atccacggca gcggaatacc gtacagcgcg gcgcgcagaa gccatttttt
    48421 ctcgccaatg cggttgcgga tgacactcca gaaagagagc gcgatgattg ccagcagcag
    48481 gaagccacac gccaccatga tacggaacgc aaagtacagc ggcgctacac gcgggatgga
    48541 gtctttggtt gcctgttgaa tctgcgcttc agtcgcatca gccacgtttg gcgtatagcg
    48601 tttcagcagc agaccgtaac cgaggtcttt cttcatgcta ttgaactggt cacgaaccgc
    48661 ctggtcggta gaaccagaac gcagttgttc gagcagagag tacgccttca tcccgttacg
    48721 aatgcgttct tcatgctgca ccatcagctc tttcaggccg ataaccgggg tatccacgga
    48781 acgcgttgca atgatgccca gtgcgtaagg gatctgaatc gcaaatttgt tcgtctcctc
    48841 ttcctgatca ggaatgccga acagagtaaa ggcagcaggc gcaggttgcg tttcccactc
    48901 ggcttcaata gcagccagtt tggttttctg cacgtcgccc atttcgtagc cggattcatc
    48961 acccagaaca ataacagaca gaacagcagc cataccgaag ctggcagcga tagcaaagga
    49021 gcgtttagcg aaggcgaagt cacgaccttt cagcatatac catgcgctga taccgaggat
    49081 gaacatcgcg ccagtcacat aaccagacgc tacagtgtga acgaatttca cctgagcaac
    49141 cgggttaagc accagctcgg agaagctcac catctccata cgcatagttt caaagttgaa
    49201 atcggacgcg attgggtttt gcatccagcc gttcgcaacc agaatccaca gtgcggacag
    49261 gtttgaaccg agcgccacca gccaggtgac acacatatgc tgaactttac ccagacgatc
    49321 ccaaccgaag aagaacagac ctacaaaggt ggattcgagg aagaaggcca tcagaccttc
    49381 gattgccagc ggcgcaccga agatatcccc tacatagtgg gaatagtaag accagttagt
    49441 cccgaactgg aactccatgg tcagaccggt agccacaccc agagcgaagt tgataccaaa
    49501 caacttgccc cagaacttgg tcatatcttt ataaatctgt ttgccggaga ggacgtagac
    49561 cgtttccata atggccagca ggaacgccat accgagcgtc agtggcacaa aaaggaagtg
    49621 gtacatcgcg gtcaaggcaa actgtaagcg cgacagttcg actatatcta acatcatgac
    49681 tccttgctca tcgcatgaag actccgagag tgaaccccgt tagaaagggt cacacgcatg
    49741 ccccaataga aatttgtttg aacactttca tcataccatg tgaatgcaca caaaaacggc
    49801 gtaaagaaag gttgcaaaca tgttaataaa aactcaaatt gatcccacgt atatattacg
    49861 ccgcaaaatc cttacaataa acaggttttt gttgaagcaa atttgcattt ttcgacggtg
    49921 atcaatttat agctaaatta ccgcctttca gccaatttga tcgagaacaa tttatctc
//
LOCUS       DAZFRN010000017        48618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFRN010000017 DAZFRN010000000
VERSION     DAZFRN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.017"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..1391)
                     /gene="ccp"
                     /locus_tag="ACHKUB_000950"
     CDS             complement(<1..1391)
                     /gene="ccp"
                     /locus_tag="ACHKUB_000950"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO6654000.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQD"
     gene            1796..3445
                     /gene="treF"
                     /locus_tag="ACHKUB_000951"
     CDS             1796..3445
                     /gene="treF"
                     /locus_tag="ACHKUB_000951"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6654001.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVHGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3496..4098)
                     /gene="yhjB"
                     /locus_tag="ACHKUB_000952"
     CDS             complement(3496..4098)
                     /gene="yhjB"
                     /locus_tag="ACHKUB_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO6654002.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4618..5517
                     /gene="rcdB"
                     /locus_tag="ACHKUB_000953"
     CDS             4618..5517
                     /gene="rcdB"
                     /locus_tag="ACHKUB_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6654003.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5566..6579
                     /gene="yhjD"
                     /locus_tag="ACHKUB_000954"
     CDS             5566..6579
                     /gene="yhjD"
                     /locus_tag="ACHKUB_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO6654004.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            6990..8312
                     /gene="yhjE"
                     /locus_tag="ACHKUB_000955"
     CDS             6990..8312
                     /gene="yhjE"
                     /locus_tag="ACHKUB_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6654005.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8546..10606)
                     /gene="yhjG"
                     /locus_tag="ACHKUB_000956"
     CDS             complement(8546..10606)
                     /gene="yhjG"
                     /locus_tag="ACHKUB_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6654006.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10676..11443)
                     /gene="pdeH"
                     /locus_tag="ACHKUB_000957"
     CDS             complement(10676..11443)
                     /gene="pdeH"
                     /locus_tag="ACHKUB_000957"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO6654007.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11675..12604
                     /gene="kdgK"
                     /locus_tag="ACHKUB_000958"
     CDS             11675..12604
                     /gene="kdgK"
                     /locus_tag="ACHKUB_000958"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO6654008.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12700..14196)
                     /gene="yhjJ"
                     /locus_tag="ACHKUB_000959"
     CDS             complement(12700..14196)
                     /gene="yhjJ"
                     /locus_tag="ACHKUB_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6654009.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETISHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14417..15703)
                     /gene="dctA"
                     /locus_tag="ACHKUB_000960"
     CDS             complement(14417..15703)
                     /gene="dctA"
                     /locus_tag="ACHKUB_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO6654010.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15886..17874)
                     /gene="hmsP"
                     /locus_tag="ACHKUB_000961"
     CDS             complement(15886..17874)
                     /gene="hmsP"
                     /locus_tag="ACHKUB_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO6654011.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17956..21006)
                     /gene="bcsC"
                     /locus_tag="ACHKUB_000962"
                     /pseudo
     CDS             complement(17956..21006)
                     /gene="bcsC"
                     /locus_tag="ACHKUB_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            21271..21687
                     /locus_tag="ACHKUB_000963"
     CDS             21271..21687
                     /locus_tag="ACHKUB_000963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654012.1"
                     /translation="MRVSPYNENESVILIYLHIDSSVRNIVIDSFSEVGLQPTVPRNF
                     CLGSGDDNIASLIVSLLRANSVLVLEALKMILSRKKIEIEVVQSGTADNPETITKIKV
                     SRPEDLEACGVIVEKICAIHAKSVNTTASNDHNKYN"
     gene            complement(21803..21943)
                     /locus_tag="ACHKUB_000964"
     CDS             complement(21803..21943)
                     /locus_tag="ACHKUB_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6654013.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSEFAAFLQC
                     KQ"
     gene            complement(22101..22391)
                     /locus_tag="ACHKUB_000965"
     CDS             complement(22101..22391)
                     /locus_tag="ACHKUB_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654014.1"
                     /translation="MQGFAKVCKNSGFRRIPKKFNALNNKRFLKLRHDRRSEKGSDDE
                     NEKIRKARTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKTATSSVARNGRKR"
     gene            complement(22809..24482)
                     /locus_tag="ACHKUB_000966"
     CDS             complement(22809..24482)
                     /locus_tag="ACHKUB_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654015.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLPKLARVEAPTEAVSAGN
                     IADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLPVPMAGSESGMFQFPPPGTLVEVG
                     FVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRDGVSQRVTVAGDWERQTDQRIQEE
                     SRERVVSADEETRSLTSRETSIQATDTTTVLGVSTLQAGAVTHITEENYSISTGGHMM
                     VVAGTCERDVEGDTLDTVGGSLTEKIKGVRHSIALAQKLVGDTVKLGNEGINVLTMLT
                     DLADVVEELADITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            complement(24485..25027)
                     /locus_tag="ACHKUB_000967"
     CDS             complement(24485..25027)
                     /locus_tag="ACHKUB_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654016.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARATPGSSRKQTPQTPGAKGGTVADET
                     PEKMTWFEEKVLKPVNDALE"
     gene            complement(24999..25724)
                     /locus_tag="ACHKUB_000968"
     CDS             complement(24999..25724)
                     /locus_tag="ACHKUB_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654017.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSHASGDVTIIMIAENTHDLFMQSMAALTAVLPVPVFTQAQRMAQAA
                     ASLSTDKMQIPITTDTLPAPVHLSVSTIRSAVSAARAAQARQLAAQRPDAAGLRRQIS
                     RFISRRAGALAGLEEGIRTLAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAA
                     LLAADSLTELGKMIHEPDRTARP"
     gene            complement(25736..26332)
                     /locus_tag="ACHKUB_000969"
     CDS             complement(25736..26332)
                     /locus_tag="ACHKUB_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6654018.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENTWHIMEDHRGKTVWRLESGSALLITEPGPLPENVTMIKP
                     DGEFCRWDGSSWVKDEAAEQAYRVSEAENKKRKLIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLSRTDTGNAPDIVWPESPSPDHHAG"
     gene            complement(26332..28392)
                     /locus_tag="ACHKUB_000970"
     CDS             complement(26332..28392)
                     /locus_tag="ACHKUB_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6654019.1"
                     /translation="MAKSVITSDFIRLKARQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLASMDE
                     RQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLRAELTEDTPMVTPAFPSR
                     IWLNVWWTGTLTSEWTTEHEIITADPDVTEERLRKTYPRDGIQQYFCAIAEIDEDGNV
                     TDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNGEDIPNKELFIINIGAPRVYDADGYVGKAGQRWTTEQFVEWL
                     NERGAFRTGSWVLRANISLSQHSVLTDTGYGDICLGGCLIEVFGELIGGIVRITTGLE
                     DSDPATKEHRAQFTGVILDRGRKTIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPW
                     PSDTPPGGYAIMAGQTFDKARYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQD
                     GIKSHTHGGSVSNTNLGTKTTSSFNYGTKTTGSAGSGSANIALRTATASPEGSGDWTA
                     YGPGSGANLTVNLPGHTHSVAIGEHAHTIALGSHGHGLTINPTGNAENTVKNIAFNYI
                     VRLA"
     gene            complement(28415..28963)
                     /locus_tag="ACHKUB_000971"
     CDS             complement(28415..28963)
                     /locus_tag="ACHKUB_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6654020.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAAQAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTEIYRRRISYAFINAQEAGEVAGFIAIFE
                     RLGIGYVELLERQPGVDWDVILVRVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     NPFIIHYGAYEGEYICWTARAE"
     gene            complement(28956..30140)
                     /locus_tag="ACHKUB_000972"
     CDS             complement(28956..30140)
                     /locus_tag="ACHKUB_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6654021.1"
                     /translation="MKGKPDVDFNQVFRESGMPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILTIVIRPVQWLRDALVDVVLPGMFVATADRSLLLLHAHDVNITPKPASKA
                     RGVIWFAKTRANDDVLIPEGTIVQTARINGVIYSMAVTEETLISSGKSGASVPVEATT
                     EGSGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSS
                     YHTDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYI
                     NEEGHHGHGDDLQCFAMPETRHRLDVTVYIRSSTTLPPDGAEGLKQDISDLIRCAFRE
                     NARFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVV
                     IE"
     gene            complement(30118..30468)
                     /locus_tag="ACHKUB_000973"
     CDS             complement(30118..30468)
                     /locus_tag="ACHKUB_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFO6654022.1"
                     /translation="MNTPSLYIDLLITDGSFTLNSGNEPQRCDNRVSIGQDVVHSIIE
                     SGIAALLIGERSPTLRSDIITRLILLVEDDERLVPGTVFINEEAPGRLHVTAETQDFG
                     RINTRVNYERETGR"
     gene            complement(30465..32651)
                     /locus_tag="ACHKUB_000974"
     CDS             complement(30465..32651)
                     /locus_tag="ACHKUB_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6654023.1"
                     /translation="MASQLDFIINLTDQLTRPLRQVQESVTGFAERSQGAFARIGVGV
                     AGLWGVAEGIRAAMDPAWEMQKALGELEAKGVGAQALTALTTQAMKLGTAFGQSSTEI
                     IRSAWHIRGALDGLSDGDLPRVTAATSTLAVAARVSSEEASGYLAAMGARFRSEFSAM
                     GAVGFAEDLAGKTAWMVQQFGVEMSTMQELIEGTKNAGADFGVSMAEQFAILGTLSRT
                     MGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRNIEGNVAA
                     QQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMTDPWDRLVAS
                     FYNIRAAIGNALYPILAPLAGRFADIGATFVRWLNMFPNIARWLGYITLTMLALGAAG
                     AVTNIVLGVFSFIMEGHKAIIKGARAVWSLYTLALTKVRSVIIATSLAARLAGVSFLT
                     MSAPVLLVAAAIAALVVVAIKFWQPVKAFIRGFIDGFTGALDAMSPVTAAFGEIARAL
                     APVWQGIKTLFGAFTDLFTPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALEVVIS
                     VIRTLVDIFAIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTW
                     NWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETAVPALAGNRISAELPP
                     GGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG"
     gene            32650..32877
                     /locus_tag="ACHKUB_000975"
     CDS             32650..32877
                     /locus_tag="ACHKUB_000975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654024.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
     gene            complement(32841..33116)
                     /locus_tag="ACHKUB_000976"
     CDS             complement(32841..33116)
                     /locus_tag="ACHKUB_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFO6654025.1"
                     /translation="MTTKNTGTIILSVAGVTLSFEPNKTAFNNLINEITMTNKVAPMS
                     TYLNRIVTPASRDALADLIARYPGCEMQIVEKINEVYSPKLEIELKN"
     gene            complement(33216..33602)
                     /locus_tag="ACHKUB_000977"
     CDS             complement(33216..33602)
                     /locus_tag="ACHKUB_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654026.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDWR"
     gene            complement(33599..33937)
                     /locus_tag="ACHKUB_000978"
     CDS             complement(33599..33937)
                     /locus_tag="ACHKUB_000978"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO6654027.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KTGKGIRNSLHTLRLAVDFNLFINGEYQTDTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGVK"
     gene            complement(33937..34689)
                     /locus_tag="ACHKUB_000979"
     CDS             complement(33937..34689)
                     /locus_tag="ACHKUB_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6654028.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRASRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCTTHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRSD"
     gene            complement(34679..34984)
                     /locus_tag="ACHKUB_000980"
     CDS             complement(34679..34984)
                     /locus_tag="ACHKUB_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO6654029.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            complement(34989..35450)
                     /locus_tag="ACHKUB_000981"
     CDS             complement(34989..35450)
                     /locus_tag="ACHKUB_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFO6654030.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            complement(35452..36597)
                     /locus_tag="ACHKUB_000982"
     CDS             complement(35452..36597)
                     /locus_tag="ACHKUB_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFO6654031.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPTPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITAKDAVNRATETRALLQSAYGRFVWFMLCVAGPGRDEAWAA
                     YVTRISAMQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRALNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            complement(36609..37319)
                     /locus_tag="ACHKUB_000983"
     CDS             complement(36609..37319)
                     /locus_tag="ACHKUB_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654032.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQTAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQSGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITVMEDQIIAGPRQQKARTWTITLPSRVFLGVTNEEFSHILA
                     RQMNAMGYGLDVDAQDIR"
     gene            complement(37316..37789)
                     /locus_tag="ACHKUB_000984"
     CDS             complement(37316..37789)
                     /locus_tag="ACHKUB_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6654033.1"
                     /translation="MNQLESLTAFITENLPSDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMDLFVSRSRE"
     gene            complement(37786..38259)
                     /locus_tag="ACHKUB_000985"
     CDS             complement(37786..38259)
                     /locus_tag="ACHKUB_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO6654034.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAAMALLAAVAEVNTTLAPVVVHWKAQGHERAADVPGARLGDETQLVAQYKKAV
                     YARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            complement(38367..39065)
                     /gene="gpM"
                     /locus_tag="ACHKUB_000986"
     CDS             complement(38367..39065)
                     /gene="gpM"
                     /locus_tag="ACHKUB_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO6654035.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTSWR
                     LHEEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAALSRTAGVKSI
                     RARIRARISALNAA"
     gene            complement(39083..40102)
                     /locus_tag="ACHKUB_000987"
     CDS             complement(39083..40102)
                     /locus_tag="ACHKUB_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO6654036.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGTPGLHTGRKKDGRFTIQLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGSENEFFNRIQNFVNRQIALDILRIAFNGTSVAENTDPVTN
                     KNGEDVNTGWHAIVKARSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEAV
                     PGVGG"
     gene            complement(40114..41163)
                     /locus_tag="ACHKUB_000988"
     CDS             complement(40114..41163)
                     /locus_tag="ACHKUB_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO6654037.1"
                     /translation="MPQPTLMTDWTCIATSGPTADGREIDPQWLIDAAETYSRNTYTA
                     MLWPCHENDTSYRQYTFNLGEVDALKVETREDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAELIPNFAKSGRDYLMGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTTPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAATGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTAAKA
                     KLEDAIKAFSTITPEKGERYRRFAARRQADTDKEEIKAIAEKLNDLTTKLSATGITPR
                     PAGTPAESEKPFDFV"
     gene            41330..43135
                     /locus_tag="ACHKUB_000989"
     CDS             41330..43135
                     /locus_tag="ACHKUB_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO6654038.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WATLLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGYEDGTAAGTCGGGEGKKRRYRKNDVSGLTAEMLDEWARKKLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFAL
                     QFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMA
                     THDKYRTTYFSTPSAKTHQAYLVWNGDDWRGDDPARRAVEFPKESAMRVGCECPDGIW
                     RYIIRLEEAVAGGLSARVDIERIRNRYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEV
                     EMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPVHEGERFRVLAVWQWQ
                     GFNFTWQADQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNR
                     LVMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDV
                     TGHADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
     gene            43132..44184
                     /locus_tag="ACHKUB_000990"
     CDS             43132..44184
                     /locus_tag="ACHKUB_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6654039.1"
                     /translation="MSKKRRNKTSRVTKKSFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLCF
                     DVEAGHVAEEKAVPVG"
     gene            44222..44485
                     /locus_tag="ACHKUB_000991"
     CDS             44222..44485
                     /locus_tag="ACHKUB_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO6654040.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(44899..45063)
                     /locus_tag="ACHKUB_000992"
     CDS             complement(44899..45063)
                     /locus_tag="ACHKUB_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654041.1"
                     /translation="MNELFIAVKGALIFTIAWIALMAFLTWENPFRHFTAGFIARIFA
                     ATVAVIWLLS"
     gene            complement(45138..45293)
                     /locus_tag="ACHKUB_000993"
     CDS             complement(45138..45293)
                     /locus_tag="ACHKUB_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654042.1"
                     /translation="MNNPRRISDKDLKKMIESAQQLLSRQNPVVEETWWENLLAALTE
                     LRERRQQ"
     gene            complement(45290..45694)
                     /locus_tag="ACHKUB_000994"
     CDS             complement(45290..45694)
                     /locus_tag="ACHKUB_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654043.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDSTNRSWLVAVRPRRPHGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLVTINRKAAIARYVHSTANRRNTP"
     gene            complement(45691..48210)
                     /locus_tag="ACHKUB_000995"
     CDS             complement(45691..48210)
                     /locus_tag="ACHKUB_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO6654044.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVDFLTNTDEGKRINSHLHHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     DRFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSTALERACDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTAPDPEQVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMACVRGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRKAINKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSPENEARITEIMRNTAIREDRAELGDDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAALHRIRQFQFFGIPPRQV
                     WRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFATYITRQGGVLIPRNTYLVR
                     TAYETAEEANDYGEFPQRIYGVRAPSLGERYTICTHPDTWELVRRKPENEDRTDEGFD
                     VECGFAAPWTRGNNCPPDQNLTINDPPERQPDERREQRLTLPEGDLGEWLRSLTPTER
                     KQLTRQLKNPPPDTETVKNGGISAPADGVRVSVALTAEPHITAQITRELDALGVQLHE
                     SAVVSVRHGARVRLADGRTAHWNDSTRRIDITPAPNTEAPQ"
     gene            complement(48207..>48618)
                     /locus_tag="ACHKUB_000996"
     CDS             complement(48207..>48618)
                     /locus_tag="ACHKUB_000996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654045.1"
                     /translation="MKNVKGLSDIQDFFNWMWAVEPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
BASE COUNT        10140 a        13541 c        13291 g        11646 t
ORIGIN      
        1 ttatcctgca tatacggcgt gtacaccccg ttcagactgt gcaggaaagc tacgatatca
       61 tccacatcct cctgcggcag ctctttgcct acctgatagc gcagcatcag tttcaccgcc
      121 ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg ccgttaaagc aacgttacgt
      181 aaaccgggta ctttctgacg caatttatca cgctcttctt tagtcacatt catacgacca
      241 atatccgccg ccgtaatttc cccaaagtta aagtcttttt tcagccccaa cggttcaaag
      301 gaacgtccgc cgagaataat accaccatga caagttgcac atttattatc tttaaataat
      361 tgatagcctt ttttctgttg cgccgtcagc gcattttcat ctccacgcaa ccatttatca
      421 aatggggaat ccggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
      481 tcgccactga aaccttgcgg ataaacttcg aggaactgcg ctttaagctg cggatctttt
      541 tccagcttag caataatttc gtcccaggat ttcgacgcca tttcaatcgg gttcaacggc
      601 ggtccacctg cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
      661 aatactgagt taaataccgt cggcgcgtta atcggcccaa ctgcgccacc aacaccaatc
      721 gatgtttttc tgccatcgac gccccccgca ttcaacgcat ggcaatgagc gcatgaaatg
      781 gtgctatcag ccgataaacg gggatcgtga tacagcgcaa agcccaacgc cactttttgc
      841 gcatcggtag gcagtttttg cgggatgggc tgcaccggtt cattgcgatg ctccggagca
      901 gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc
      961 cgctcttcat cactcacctt acccgcccag tgtagcgcgg tataacgcgt tggtggcata
     1021 gtttcatact gcatcaccca ttcaatctta ttcagatcgc tttgcgaaac gggtttatca
     1081 gccagcagtg ccgcacgcac ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg
     1141 taatccatca actgtttcgc gccaggaata taataatagg cgggtaattc tgccgaaggt
     1201 gtgtggcaat agtcgcatcc tttttcgcgg agaaagccta aaaccttatt attttcactg
     1261 acagcagatg cctgaacatc ggcctgttta ctgcgtttat tatcgtggta ccacacataa
     1321 ccagataacc ctaaatagca aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag
     1381 accattttca ttattcttcc ctcacggtgg tgagttattg tgacccagaa aattttccag
     1441 aatgctgcaa acgtctctgc gagacaaagc catcataaaa aacagggaga tacgcttttt
     1501 gataggaatc aatcaattac atgaatataa actatcaata agatagcctg tgcctattac
     1561 ccagatcagg aatggcatat catgattgcg gcgagagcag gattgaatgt tctgaaaatg
     1621 aaaaacaaca cgaaatccgt atttttataa attccatgga tattagtgcg ttatattttt
     1681 tgacaggtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
     1741 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
     1801 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaagtcg atctctgcta
     1861 tgagctggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     1921 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     1981 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
     2041 acccaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     2101 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
     2161 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     2221 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctac cgcagtcata
     2281 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
     2341 ggggctggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     2401 gatgatcgaa aactatggtc acatccccaa cggcaaccgc acctattatt tgagccgatc
     2461 gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacacggtgc
     2521 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     2581 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     2641 ccgttattgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     2701 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggagcggc
     2761 ctcaggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     2821 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     2881 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcgctgttcc gccagaaggc
     2941 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     3001 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccgct
     3061 ctatgtcggc atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tacgcagccg
     3121 gttactgaca cctggcggga ttctggcaag cgagtacgaa accggtgaac agtgggataa
     3181 acccaatggc tgggcaccgt tacaatggat ggcaattcag ggatttaaaa tgtatggcga
     3241 tgaccttctg ggtgatgaaa tcgcgcgcag ctggctgaaa acggtgaatc agttctatct
     3301 ggaacagcac aaaatgatcg agaaatacca tattgccgat ggtgttcccc gcgaaggcgg
     3361 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     3421 aattggtttg tacggcgaac cataatattt ttacagccag ccgctaactt cctgctggct
     3481 gtaaaattat cctcttcagg aggagatatt taacatcatt gccgcctggg tgcgattttt
     3541 cacttccaga cggcgataca gggattccag atgcgctttt accgttccgg tactgatatt
     3601 cagcgctctg ccgatctcct tatttgattc gcccgccgct aacatggtta aaatctcccg
     3661 ctggcgggcg cttaacgatt tgagatcttt aatgtccttt tccggcgtcg tccgccagtc
     3721 tccaggcaga aacatcatcc ccatcgccgc actatttacc gccaacgcaa atgtctcgac
     3781 ggttgaatca cgaggcacaa tggccagcac attaaaatga ataacttcct gtaaccaccg
     3841 tttattgcaa tccgtcgccg tgattaacac cttaacctca ggaaactgca ccacggtttt
     3901 ttgcagcaac cagtagcaaa actcaccatc ctgatcgcca tcgagcataa ctaaggcttc
     3961 agggtaactt tccagctttt gccataactc gtctgcctga ctggccccct gaatactcac
     4021 tcctggaata cgctgctgta aactgatttt cattccatga ataaatattg actgcctgtc
     4081 aaacatgact atttgcataa ctgaatctcc acctgaatac gttaaaaaga cttaagtagt
     4141 ggaagggtat tacccgcgag aaaaaataag aattcgccat ttggcggtgg ccattctaca
     4201 gagatgacgc gtagaaaata gttaccgata taaatagtta cagctaaacg cctgaaatta
     4261 catgtcgagg gcactattta aaacaatttt gaggatttcc ttatattggt ggttagtacg
     4321 catgcaatta aaaatgaaac tccgcgacca caagcaaaaa taacaaacgg caatgagaca
     4381 aaaataagca caaatagcca acacgtcctc tattcacttt aaagggaatc gctgaaaaat
     4441 acgctctgtt tagggggatt cacctttctc agaaagctat tccgcccttt tcctgctgag
     4501 aaatcgccac attcgtcatg acaacattgt gaaacccggc attagatgtt agaaaaaaca
     4561 ataaacaatg cgatatgcgc gttactgttt cgctgtgaac aacaatcggt caaagaaatg
     4621 gataaaattc acgcaatgca gttgttcatc aaagtcgcgg agctggaaag tttttcccgc
     4681 gcggcggatt tctttgcttt gccaaaagga agtgtttcgc gccagataca ggcactggaa
     4741 catcaacttg gcacccagct tctccagcgc accacgcgac gggtcaaact cacgccagaa
     4801 ggcatgacct attatcaacg agcaaaagat gtgttgagta atctcagcga actggacggt
     4861 ctgtttcaac aggatgccac cagtatcagc ggtaaattac gcatcgacat cccgccagga
     4921 atcgcgaaaa gcctgttact gccgcgcctg tcggaatttc tctatctgca tccgggaatt
     4981 gagctggaac tgagtagcca tgaccgtccg gtagatattc ttcatgatgg ttttgattgc
     5041 gtgatacgca ctggcgcgtt accggaagat ggcgttatcg cccgtcccct cggcaaactg
     5101 accatggtca actgtgccag tccgcactat ctgacgcgct ttggttatcc gcaaagcccc
     5161 gatgatctga cttcacacgc aatagtgcgt tatacaccgc acctgggtgt acatccgtta
     5221 ggttttgagg ttgccagcgt taatggcgtc cagtggttta agtctggcgg catgttgacg
     5281 gtaaacagta gcgaaaacta tctcgccgcc ggtcttgccg gtctggggat tattcagatc
     5341 ccgcgcattg ccgtgcgcga agccctgcgt gccgggcggc ttattgaagt gttgccaggc
     5401 taccgtgccg agccgctctc cctttcgctg gtttacccgc agcgtcggga gctttcccgg
     5461 cgtgtaaacc tgtttatgca gtggctggct ggcgtaatga aagagcacct ggactgaccg
     5521 actatacttt ttaagaatga ccacgacaga aggacaaaag agcggatgac gcaggaaaac
     5581 gagatcaaac gtcccaccca ggatctggag cacgagccga ttaagcagtt ggataatagc
     5641 gagaaaggcg gcaaagttag ccaggcgctg gaaaccgtca ccaccactgc cgaaaaagtc
     5701 caacgccaac cggttattgc gcacctgatt cgcgcgacag aacgctttaa cgatcggctg
     5761 ggtaaccagt ttggtgcggc tatcacctat ttctcatttt tatcgatgat cccgattttg
     5821 atggtgtcgt ttgccgcagg gggctttgtg ctggcctccc atccgatgtt gctacaggat
     5881 atcttcgaca aaattctgca aaacatcagc gatccgacgc tagccgccac gttgaaaaac
     5941 accatcaaca ccgccgttca gcagcgtacg actgtagggc ttgtcggcct ggcggtggcg
     6001 ctttattccg gcatcaactg gatgggtaac ctgcgtgaag cgattcgtgc ccagtcgcgc
     6061 gatgtctggg aacgctcgcc gcaagatcag gagaagttct gggtaaaata tctgcgtgat
     6121 tttatttcgc tgattggttt gttgattgcg ctgattgtga cgctttcgat cacctcggtt
     6181 gccggttcgg cgcagcaaat gattattagc gccctgcacc tgaacagcat tgagtggctg
     6241 aaaccgacgt ggcggttgat tggcctggcg atttccatct tcgccaacta tctgcttttc
     6301 ttctggatct tctggcggtt gccgcgccac cgtccgcgca aaaaagccct gattcgcgga
     6361 acatttctgg ctgctattgg ttttgaagtg attaaaatcg tgatgaccta caccttgcca
     6421 tcgctgatga aatccccctc tggcgcagca tttggttccg tgctggggct gatggcattt
     6481 ttctacttct tcgcccgttt gacgctgttt tgcgcggcgt ggattgccac cgccgaatat
     6541 aaagacgacc cgcgaatgcc agggaaaacg cagccttaaa ataggccgga tgcggcgtaa
     6601 acgccttatc cggcatacga ttttattcgg cctggctccc cgtaggccgg ataagatgcg
     6661 ccagcatcgc atccggctat aatgcgcaca taacctcttg aaactcttcc ccagagcctc
     6721 ttcagccatc tatttgggag caaacaattt cattccaact cataacccca gcatataaat
     6781 ccagttggta acttttattt aacctgaaac cagttttatc cactatttat aaaattatgt
     6841 gaagcatttc atagaagaaa aatcactggc ctaaacatta tccccttttt gcctggtttt
     6901 tgaccatttc cgcgatttgt tacacattga aatatcactt ttgctgtgcg taatatggct
     6961 attcgttagc caaaaaataa gaaaagatta tgcaagcaac agccacaaca ctcgaccacg
     7021 agcaagaata cacgccgatc aactcgcgta ataaagtcct tgtcgcctct ctcattggca
     7081 cggccattga gttcttcgac ttttacattt acgccactgc ggccgttatt gtgtttccgc
     7141 atatcttctt cccgcagggc gatccaacgg cagcaacgct acagtcgctc gccaccttcg
     7201 ccatcgcctt cgtcgcgcgc cccattggct ctgccgtttt tggtcatttt ggcgatcgcg
     7261 ttgggcgtaa agcgacgctg gtcgcctcgt tgctaacgat ggggatttcg accgtggtga
     7321 ttggtctgct gccgggctat gccacgattg gtattttcgc cccactgctg ctggcgctgg
     7381 ctcgatttgg tcagggtctg ggcttaggcg gtgaatgggg cggcgcggcg ctgctggcga
     7441 ctgaaaacgc cccaccgcgc aaacgtgcac tgtatggctc cttcccacag ctgggcgcac
     7501 cgattggctt cttctttgcc aacggcactt tcttgctgct ttcctggcta ctgaccgatg
     7561 agcagtttat gagctggggc tggcgtgtgc catttatctt ctcggcggtg ctggtcatta
     7621 tcggcctgta tgttcgcgtg tcgctgcatg agtcgccggt gtttgagaaa gtcgctaaag
     7681 cgaaaaaaca ggtgaagatc ccgctgggta cgctgctgac caaacatgtt cgcgtaaccg
     7741 tactgggtac gttcatcatg ctggcaacat atacgctgtt ttacatcatg acggtctact
     7801 ctatgacctt cagtaccgcc gccgcgccag ttgggcttgg cctgccacgt aacgaagtgt
     7861 tgtggatgct gatgatggca gttattggtt ttggcgtgat ggtgccagtc gccggattac
     7921 tggctgatgc ctttggtcgc cgtaaaagca tggtaattat caccacgctg atcatcctgt
     7981 tcgcgctgtt cgcctttaac ccgctgctcg gttctggcaa cccgattctg gtttttgcct
     8041 tcctgctgct ggggttaagt ctgatggggc tgaccttcgg gccgatgggc gcgctgttac
     8101 cagagctgtt cccaacagaa gtgcgttaca ccggagcatc gttctcttac aacgtagcgt
     8161 cgattctcgg ggcttccgtt gcgccgtata tcgcagcctg gttgcagact aactacgggt
     8221 taggtgcggt ggggttatat ctggcggcga tggctggctt gacgttaatc gccctgctgc
     8281 tgacccatga gacgcgacat cagtcgttgt aagtatcgtg tgggcagcca ccctcacccc
     8341 agccctctcc ctgaaaggga gagggagaaa agcggagcga gatgttatat cgcgaacata
     8401 acagccctcc cccagaaagt gaacggaaaa gcggtactcg ttgtttcaac acgcacaaaa
     8461 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacacgctg ctcccgaact
     8521 caccagcaaa cacaatccaa cctcatcact tcttcatctg cgacaatatc gtccgacact
     8581 gattagcctc cccttcggaa ggtgagatca gtgccagtaa cgccgccgcc ggtgttacca
     8641 gcgttgccag tgccgcagca acagcaccac gggcaatcaa cggcccggct ttcaccccag
     8701 cctgcggatt tttaaacgtc ccacgcacat acagcggcga acgcagtgtg ataatccgaa
     8761 tccctttact ctccggatca atagtcaaat ccagctgttc cgaagcaaaa cttgccgtgc
     8821 cggtaacatt aatcaacgcg ttctcagtat cgaaagcaaa aatctgcggg cgcgccacgc
     8881 cgttggcaat attcagattc gccgccgcgc agttcacccg cacctcatcg tcaccaaata
     8941 tcgcaccgac aatgtagttg ccgacattca gcccaacaat ctccatcagg ttgcggctca
     9001 ccagcccgtc attcatcaac agtttcaggt tgccgttact gttgcctaaa agcgccgcca
     9061 ccgagttacc gctaccgcgt agttccgcgt caccgttcat ttcccccagc gtcttctgca
     9121 tcagttccac atcgggcatc agttctttca gtttcagtcg acgagcctga atatctgccc
     9181 gcccctgcat cggcttttta tcgccttcca gatggatatt cgccgcaatg ctgccgcccg
     9241 ccatgccaaa tttcagcggt tgcaggcgca ggtcagcatt tttgaggatg atatgagtag
     9301 aaagatcgct aatcggcagg ctactgccat gctcaatgcg ccgccctttg aagcgaacat
     9361 cggcatccat aacgtcccat ttatcggttt cgaagcggtc ataaggcagc actttacccg
     9421 caggctgaac gcttttttcg cccttcttct gttcagaccg tttcgacttt tctgcccctt
     9481 tcccggagtc aacgccaatt aacggtccca ggtccgccag ccgcaattgc cgcgactcga
     9541 catcaccttc cagttttggt cgtggctttc cggtggtgta gaccagagaa ccgtggatat
     9601 cgctatcgcc aattcgccca ttaaaaccgc gataatcaaa gaccgacgat ttttcagtgt
     9661 cgattttcgc taccagccga ccatccgttt caaacggcgg ggtatcgggc agcagaacgc
     9721 ccgtcagttc atagagatca cccagtgaat cgccagaaaa tttaagccgt aaatcgacac
     9781 cgcccatctt cattgggtca ttcacgacgc catcaaaagc aacacgggtg ttaccagagc
     9841 ggaaatcagc ctgtaccgga aacggcgtcc cttcgccacg cagcgccagc ataccgccta
     9901 tttttcccgt acccgtgagc ggttcaccgt tataacgtcc ctgcgccttc aggccaaaaa
     9961 cgtaatcgcc caccttttct ttatccgctt tacctttcga tccagtaact tcgctgaacg
    10021 gcagcggctt gcctaacgga tcaacaaaaa tctccagatc cgctttgctt actttgtcat
    10081 caatagcgat ccgcccttga tcgaaaagaa tattatccag ccgaaacgac catgccgacg
    10141 gctttgcatt cgcgtctttg ttatcatcgt tggcgagatt aaacgtccag ttattgttct
    10201 tttcggagag gcgaatcagg cgcgcgtcgg gcttttcgag cttgatccac ggcagccaga
    10261 ccgttttggt cagcagcgcc agcggggcca gcgttgcctc tacgcgtggc aaatgcacca
    10321 tcgtgacttc gggaatatcc ggtggattgc caagaatgat gtcttccgcg tgtacatggg
    10381 gccacggcac ccagctgcgc cagccagttt cttgtttttg ccgctcccac accacgccca
    10441 gatcgccacg gatagcgaac ggacgattca actccgcaga gactttctgg ttgatggtcg
    10501 gtttgagtcg attccagtca aaagttgcaa tcaaaatgat cgccacgaca atcaacaaca
    10561 agaaagcccc tgaaatcgca gcggttattt tgcctgcctt gctcatcgtt cgcttcctca
    10621 tattcttcct gtgccagtcc taaagatagt ccagccaggc ggaaaatgag gcagcttata
    10681 gcgccagaac cgccgtattc agcgtttcta tcggtgccgg gcgtgaaaga aaccagcctt
    10741 gtgcggcgaa tgcgggcgag ttctgaacat cacgccactc ttccggtgtt tctacgccct
    10801 cgacaatcac cccgcgacaa tagcgattca ttagatgtaa aagctgagaa aagagtgtgc
    10861 ggccttccgg cgactgacgc agcatcacga acagctctcg cgcgattttg atgtagtcat
    10921 aacgcacttc acttagcgca gagaaatttg ccatcccggt accaaaatca tccagccaca
    10981 gcgggccaaa ttcacacatc gaggcaaagg ttgaatcttt cggcaggcgg atgtgttcca
    11041 ccagttcgaa acgcagccag ggaagacgct caatctggcg caggattttt ggttgctgac
    11101 gcagggcgat gagcgtaggg ccatcaatat tgaccgatgc cagcaggccg tgctctataa
    11161 agaagtcggc tttttgcgcc aacaaatcaa tctgctcttt cacaacctcc atccgatggc
    11221 tgacggtgat ttcagtaaaa tagcgatccg gcggcaggcg ttgcgaaggg ttcaagggat
    11281 gcgtgaccac cgttaatagc tccacggcca ttaaccgccc gcatgtttga tagatcggct
    11341 gccaggtgta agcacgctca cactgcaacc aaaaacgccg ttcctgcaag ctctcgatgc
    11401 ttgcttcagg gttgcttatt cgctggataa cctgccttat catcaaagat gtcctgtgtt
    11461 taaaagtgat gctgcccgga ctcgacaaag attatcggcg cgcaagggca gaactttacc
    11521 gcctggccgg ggtaaatttc ctcactccac aacggaatct ttcaggaata cgttctggct
    11581 caaaaaaata atggaacact gttttaatat tgttgaccag caaaccacca cagcgcaaac
    11641 taacgctaat tttttacaga tcaggttcac gactatgtcc aaaaagattg ccgtgattgg
    11701 cgaatgcatg attgagcttt ccgagaaagg cgcggacgtt aagcgcggtt tcggcggcga
    11761 taccctgaac acttccgtct atatcgcccg tcaggtcgat cctgcggcat taaccgttca
    11821 ttacgtaacg gcgctgggaa cggacagttt tagccagcaa atgctggacg cctggcacgg
    11881 cgagaacgtt gatacttccc tgacccaacg gatggaaaac cgtctgccgg gcctttacta
    11941 cattgaaacc gacagcaccg gcgagcgtac gttctactac tggcggaacg aagccgccgc
    12001 caaattctgg ctggagagtg agcagtctgc ggcgatttgc gaagagctgg cgaatttcga
    12061 ttatctctac ctgagcggga ttagcctggc gatcttaagc ccgaccagcc gcgaaaagct
    12121 gctttccctg ctgcgcgaat gccgcgccaa cggcggaaaa gtgattttcg acaataacta
    12181 tcgtccgcgc ctgtgggcca gcaaagaaga gacacagcag gtgtaccaac aaatgctgga
    12241 atgcacggat atcgccttcc tgacgctgga cgatgaagac gcgctgtggg gtcaacagcc
    12301 ggtggaagac gtcattgcgc gcacccataa cgcgggcgtg aaagaagtgg tggtgaaacg
    12361 cggggcggat tcttgcctgg tgtccattgc tggcgaaggg ttagtggatg ttccggcggt
    12421 gaaactgccg aaagaaaaag tgatcgatac caccgcagct ggcgactctt tcagcgccgg
    12481 ttatctggcg gtacgtttga cgggcggcag tgcggaaaac gcggcgaaac gcgggcacct
    12541 gaccgcaagt accgttattc agtatcgcgg cgcgattatc ccgcgtgagg cgatgccagc
    12601 gtaaacgcag catatgcaaa aggctggata aagtgttacc cctctatcca gctcatccat
    12661 aaatacctga tggttgtgct aactcatcac catcaggcat tactgtgcag gtggaatatc
    12721 cgtcacttca ggatgtacgt catccgtggc gacagaggtg gcggcagcag cagtcgatgg
    12781 ggccatgatt tgatcccagg ccgcctgcaa cgctttcatg ttaaattccg gctcgccttt
    12841 cggctgcagc agtattaacg ccatatcatt cgacaactgc tgacgcagat cctgatttaa
    12901 catctccacc gtcaggctgt taaggaaatc ctgccgcagt ttctgatact gctccggcgc
    12961 gatatcgacg acctgatttt gcaacgaacg catccgctga cccatcagaa tatcggtatc
    13021 agcccgcgca taggcggcaa acagtttctg cagctccagt ttcttttgcg ccactaacgc
    13081 attgaactct tcttccggca gacctttatc gcgaaccttc gccagttcac gcgccaccag
    13141 attaaggttg ctgttcagct tgtcgtttgg tgattcgatg ttgatggcac actgcgcacg
    13201 cagatacagc acacggcagt caaatccaag accgatgtct ttgctgttac tggcgcttaa
    13261 cgcttgctga acatgccaga acagcgcctc gcgggccagg tccgcacgcc agtagcgcag
    13321 cagtgcggct gattcacgaa tcggctgcca cggcgtatcc cacatgatag ataaccggtc
    13381 ctgacgcacc gcgtcagtca taatactcac cgcttccgca cgcagcggag aaagcgtcgg
    13441 caccggagct ggcgtttcac gtttgccttt cagttcgcca aacgttttgt ttatttggtc
    13501 gacaaccgag cgcgcatcca cgtttcccac caccagtagc gtcattgcat ccggggtgta
    13561 ccatttctga tagaaatctt taattttttc cgcttcaacg ggttgtttca gcggatcggc
    13621 aggatcgtga cctaacaagg ttgatccttt cagacgataa cgccaccagc cctctttagt
    13681 atcggcaggc caggttgcca ccatgtcctg actttgcagc gcgtggctga tggtttctgg
    13741 cgtgatagtc agtttgccag tggcatttgc caggtaagag agcgcctctt tcagcaaatc
    13801 gttacggtta ttgggcaaac tcagattaaa cagcgtagtg tcatacgaga caattaccgg
    13861 cggcatcggg cgtttagggt cgatcccctg ctgccacaat gaacgcgcct gtgctgcgtc
    13921 aaggccacca ctttgcgtta gcgcaatacg agggatggcg tgactgtaac cgctctgttg
    13981 tgtactttcg gcgagcgaac cggtattaac cagcaggcga atttcaacac gatcgctggg
    14041 acgctggggg gtggtcagca cttgccactg taaaccgttg gaaagcgtcc cctgttgcca
    14101 tgctggatca ggctggagcg catctgcctg cacatagcca gcagtggcca tcatcagcaa
    14161 accgcccgct aaaagtcgaa tttttgtgcc ctgcatgtga acccctgatc aactatcctg
    14221 gtaataaaaa aactgcccgt tgtcggtcag tctaaatgac gttaaaaaca cttcgtgtta
    14281 gaccgcaaga acatgaaaat gtcacggaag aagtgaaata aacctgaact cacccaggtg
    14341 gtaagttcag gcacaggggt caattatgcg caaacacccg cactcgggga agggagtgcg
    14401 ggcataagtg atgagattaa gaggataatt cgtgcgtttt gccatccggc gcacgattat
    14461 tcagcacatc gtccagtttt ttgtggtcca gttctttcac ccacttagca acgacaatgg
    14521 tcgctacgcc gttaccgacc aggttagtca gcgcacgagc ttctgacata aagcggtcga
    14581 taccgaggat cagcgccaga cccgctaccg gcaaatggcc caccgcagag agcgtcgccg
    14641 ccagcacgat aaagccacta cccgttaccc ctgccgcccc tttagaagaa agcagcaaca
    14701 cgattaacag cgtgatttgg tggacgatat ccatctgact gttagtggcc tgggcgataa
    14761 acaccgccgc cattgtcagg tatatcgatg tgccatcaag gttaaacgag tagcctgtcg
    14821 ggatgaccag ccccaccacc gatttgcggc agccgagttt ctccatcttg tcgagcatac
    14881 gcggcagcgc cgactcggaa gatgaagtcc ccagtacaat cagcagttct tcacggatgt
    14941 agcggataaa tttgaagata ctgaaaccag tcgctttagc gattgaaccc aataccagca
    15001 ccacaaacag gatacaggta atgtagaaac agataatcag ctgccccagt tgcaccagtg
    15061 tgccgacgcc gtatttaccg atggtaaacg ccattgcccc gaacgcacca ataggcgcca
    15121 gacgcatgat catattgatg atgccgaaga tgacctgcga gaaactttcg atgacgttaa
    15181 aaatcagttg gcctttgctg cccagacggt ggagcgcaaa accaaacagt acggcaaaca
    15241 gcagcacctg cagaatgtta ccgctggcaa atgcgccaat gacgctcgcc gggatgacat
    15301 ccataatgaa ggcgacaatg ccctggtctt tcgcctgatc ggcgtaaacc gctaccgctt
    15361 tcgcatcaag cgttgccgga tcgacgttca ttccggcacc aggctgcacg acgttaacga
    15421 tgataagacc aataatcagc gcgatggtac tgacaatttc aaagtaaagc agtgcgactg
    15481 cgccggtacg accgaccgcc ttcatgcttt ccatgcccgc aatgcccgtt acgacggtac
    15541 aaaagatgac aggagcgatg atcatcttaa tgagcttaac gaagccgtcg ccaagcggtt
    15601 tcatttgctc gcctatttca ggatagaaat ggccaaggag aataccaatg gctatcgctg
    15661 tcaggacctg aaagtaaagg cttttaaaca gagaggtttt catagggtgt cctttagtaa
    15721 aaccacaggt cttgtaaggt tatggggtac ctgcggcctt aaaataacac ccagacaaca
    15781 tcacagaaat gtacctggat cataattgaa acaaaaaggt taaaaagttt gagctggctc
    15841 gcacaaacca gcacttttta aagttttgta atcagtttgg ggtagctact tttcttccag
    15901 gtaactctct tcgaagattt caatagggag tgggcgagca aaaaggaagc cctgggcaat
    15961 accaacgccc gcttttgcca gccagtcgcg ttgtgcttca gtctccacgc cttcggcaat
    16021 catttgtaag ttcaggctct gcgccagcat gataattgca gcaatcatgc tgctatcttc
    16081 cggcaagcct tcaacaaaca ttttgtcgat tttcagtacg tcgattggca acgatttcat
    16141 atgctgcagc tgacgcagcc ctgcgtagcc catgccgaaa tcatccagcg ccacccgaac
    16201 tccggcattg cgcagcggac ggaggattgc caccgcagca tgagggtcgt caatacgtcg
    16261 gctttctgtc acttccagaa tcagtgttcc cggctgaatg cgatagcggg ttaacagttc
    16321 cagcatatcc gccaccatat tcgggtgcat cagttgcagc gcagagaggt ttaccgacaa
    16381 gggcagcata atgccgcgct cttgccaggc tgcaagcaat cgacaggact cttccagcac
    16441 ccagtgaccg acggtaacca tgagcccaca gcactcaatg cgatcgatta agccatccgg
    16501 caggtcccaa ctgccatccg gttgctggat acgcagtaac acttccgcac tgaccagttt
    16561 accgctggtc atctcgacct gtggctgtaa ccaaattgca aactgatgat tttccagtgc
    16621 attaaggata tcgctctctt ccgtcaaccg cttctgggcg gcttccatct gctgcggatc
    16681 aaagaactga atctgattct tgcctttatg gcgagcggta aatgccgcag aaatagcgcg
    16741 actgtaaagc tgttcggcgg tgagatcgcc gtagaacatc gccacgccaa tgctacagtg
    16801 cggacggagt tgaatacgtt caatcggcag gcgctcgctc atgatagtga gcacttgctg
    16861 acctaaggtg attgcgtgcc acggttcctg tacaccgttg gcaatgacag caaagtcata
    16921 accgctaatc tgcgcgagga tcatacgtgg cgacagtacc gatttgagtt tttccaccag
    16981 cgtcagcagc agaatttctc gttgcgcctc tttcagcacg cccgcagtat cacgcagggt
    17041 ttcacaggtg atgatcatca gcgcggtggt ttgtttacgc gcgacaacct gctccagcat
    17101 ctccatcagc aaggctttgt tcggcaaatc cgacaccggg aagcgcatcg cattttcgtt
    17161 ctgttcttca taatggcgct gcagcaattg ctggttgagg ttgtaactgc gcaccaacat
    17221 accgatttca tcgtcctgat gcagacgcgg taatgccagt tggtgaccaa caagctcctg
    17281 ggctgggatg gcgttaagtt cgcgagcaat attgcgtaac ggatgcaaaa tcaggcggtt
    17341 aatgcaccag ctgatggcga cggtcaggat aagcgacaaa agtaagtaaa tggtcactaa
    17401 cgttgagagg gtgctcatca cgaacttata catacggaag gaatccgcct gtagtaccag
    17461 ataggcaatt ggctgcgggt ttgccggacg ttcgagcgag taaacgccca gcgagatttg
    17521 aaccggtagc tcaaacaggc gagtaaccat taccggcacc gggcgctctg gaataaaact
    17581 tttacgcagc gcctggaact ggttaggcag cactacatcg gcacggctga ccacgcctgc
    17641 cggtttaatg ctggcaagaa tagcttccgc ttcgggaata tcgcctttca aaatagctga
    17701 agataagggt tcgcggacag agcgagcaat gctttccagt tgcgtagccg tgttatagcg
    17761 attctgctgg accagatgga acagcaaaac ggtgcaaaaa ataaaaacga acaccaggac
    17821 aacggctgcc accattgcca tctgcttgat tgttaacgag cgacttacgc gcaaattgtc
    17881 tccccccgga aatcccagat tgccgcccga gtatacccga tcgcagcggc attaagagag
    17941 gcgctatctg aaaacttacc agtcggcgta aggtatcagc ggctgcggcg gtaaatccat
    18001 gtcaccctgc catccggcgg cggaataacg tacgtagagc aggaaatggc tgggtgcgta
    18061 atctttcgcc tgctggatat caattgccgt gccaacaaac cagttggaag taacacgtcg
    18121 ttcaagtaat gcccgcgccg tgtagccgaa gccctgactg ctgccgccat cgttggattg
    18181 ccgcgcagct tcttcctgcc agtcggtcgg gatcagattc atcagcggat aacgcggcat
    18241 ggttttggtg cgtgaatgcg accacgagcc agacgccccc agctcccacg accagttttc
    18301 cgtgcgctcc cgccacatca ccggtatggc aaacgacagg tattcctgcg gactgtagta
    18361 accgccctga ccgagtgagt agccactcag atctttgtcg taatgccaga tcatgttgtt
    18421 caggccgatt gtgacgcggc gattgttctg gttaatgacc ttatagtaat agcccgtcat
    18481 ccagcgcacg cgccagttat cttcgacatt tttaccggtt aactggtcgc cactaagcga
    18541 tgcccagacg ccgtttgctt cacctttatc gtagctcaga cttagcccca caccgtcggc
    18601 acgtacgcca ccccattttt tcccggtatt gctcggggag tctttttgcc caccaaaggc
    18661 cagcaaagaa ctggagatgg gccgacggtg ggcgttaacg gtgtaaccca gcggcccgat
    18721 atcatcgctg taactgatgc cgccgaccac atccaccacg ttgaagccca tcggcgtggt
    18781 accgatatcc cagctccaga cgtcatttcg ccagccgacc gccacgctgg caccggaatc
    18841 cgactggctg cggttgccgc tacagtcctg taatgtacag gtgccccagt tgtcatccca
    18901 tttgccatcg gcattagtgg agaaactgcc gacgttcata ttgacgaaat cactgcgaaa
    18961 gaacatccgc ccgtcagaat acggcgcatc cacctgcaac atggtagtgt gcgctttcag
    19021 atcggagtaa ccaccggtgc cgctcgaacc ccagtaatcg tgctcgaggg tgacgttaag
    19081 atcctgctgg cgatagaggt ccgccgcatc gctgcgcacg ccgcgtttca gccagtcatc
    19141 tttctcgtcg ttacgggtca gtcgggtgaa tgtgtcgtta tcctgcggac gcgtcgtggt
    19201 cacaccggat gcgaccatgg cgtctttgta ggtttccagc gcctgctttg gatcgcccgc
    19261 ctgcgcttca aacttcgcac cgtcgcgcag caccatcgcg ctttccatcg acggtggctg
    19321 agattttgcc tgcgggatca acttattaaa cgtctgctgc gccgctgcgg tatctccaag
    19381 ctgcgcctgc gccagcgcca cgcgccgctg tgtgttcagc gaggcgttat cggtagcggg
    19441 cagtttcgcc agctggctac gtgccgccgc tgtgtcaccg gcagcaatat ccacttccgt
    19501 cagccccaga atggcatcgg cgttagttgg ctccagcgtc aggacattct gatatgcagc
    19561 gcgggcggcg gtgtaatcac gtcgctgctg cgcccagtca gccagcgtga ggtcaatacg
    19621 cgtggaaggt ggttgctgac gcagcatcgc ttccgcttca gcttctttgc cgctttctcg
    19681 caggcgatta gcggtttcca tcacctgatc gctttgcagt cgattaacca gctcctgaat
    19741 attgctgttc cactgcgcgc gcggcaggct gttgatatgc gccagcgccg ctctgtcctg
    19801 gtcatgacca gagaggtaca gcccgtaagc gtaaacctgc tccgggtcgt tcggcttatg
    19861 ctgcgccaga ttgcgcatta acgtatcggc ctggctgcgt tgtccggcct gccagagatc
    19921 ctgcgaaagt cggtaagtaa tccatacgct gccggggtcc agcgccagtc gttgccgctg
    19981 aagtgctgcc gcctgcgccc atttgccctg gttttccagt gcctctgcct gctgtgccag
    20041 acggtcgttt tgcaggctgc gttcgatatc atcaatgcta cgccgctgac tggcagagag
    20101 cgaggcgata aacgcttcag ctttttctgg cgattgctgg cggtaaatat ttgccagccc
    20161 gcgcacggcg ttagtgttgc cgctgtccat acgcaaggtc tgctgataat aacgttcggc
    20221 ggcgggataa tcttttcgcg ccatcgccac atcgcccagc cccagcactg cataactgtc
    20281 ggtgttatcg acattacgcg cctgctggaa caggcgttct gcccggtcag gattattggc
    20341 tttcagcgca gcatcgccct gctggatcgc cagccagtag cggtttactt tcagcagact
    20401 gttccatttg tcgttgttgc tgctgtgcgg gtccagtgcg agggcttttt ccagattcgc
    20461 cactgcattg gcgcgatcgc ctttctgaga atacgcctgg cccagcgccc ccagagcttc
    20521 actgtctttc gggttcgccc gcaccgcctg ttgtagttcg ggaatggctt tacccgccat
    20581 accagagtcc accgccgcta aaccttgcgc acgagcgcgg aaagcaggat cggccagctg
    20641 tttttgctgt tctgccagtt gcgattgcgc agccgccacg ctatcgccat cactaaagat
    20701 cgagagatat tttttcagcg ccgacacact ggcatcactg acgggcatgt ctttaatctg
    20761 cccgtaccag attttagagg ccccttcgcg cccggcgttc gattttgcca tctgttccag
    20821 gacggcaaaa ccttcgtcac gccgatcgct actaaacagc aatagcgcca gattgttttg
    20881 caggcccgta ttgcccggcg tatccgcatt aatgcgtttt agctgattaa tcgcttcgcc
    20941 acggcgagcc ggaattttcg ccaccgtact ccagtactcg acggcaatgt caccttccgg
    21001 tggcgcgaaa actgatgtaa tgcaatgaaa aataaaggat tatttttcgc attgtccaca
    21061 ttttgccata gcgatacacc ttagcccgta atgattcgcg ggctaagcat atacaaaatg
    21121 ttaattctaa taagggagag gcttatgttt gatagggaaa taataaacga aaataaagat
    21181 ttttattttt atgtgtccag ataacgtcca tctttgaata aaaagttcac agagttgatg
    21241 ctgtgggttt tatttttgtg gagagcggat atgagagtaa gcccctacaa tgaaaacgaa
    21301 tctgtaatat taatttattt gcatattgat tcttctgtca gaaatatagt cattgattcc
    21361 ttcagtgagg tggggcttca gccaactgtg ccccgtaact tctgccttgg atctggtgat
    21421 gacaacattg cctcattgat agtatcttta ctcagggcca actctgtgct ggtactggag
    21481 gcgctgaaga tgattctttc aagaaaaaaa attgaaattg aggttgtgca aagtggaacg
    21541 gctgataatc ctgagactat tacaaaaata aaggtttcac gcccggaaga tcttgaagcg
    21601 tgtggtgtta tcgtggaaaa aatttgtgct attcatgcaa aatcagttaa taccactgcc
    21661 agtaacgacc acaataaata caactgaaca aaaaggcccg catgtcgcgg gcaaatacgt
    21721 ctgtaatcca acggagaaag ggtgccatat cgaccgaacc cggcaaccgg atagcggggg
    21781 ctttcacccc cgctgcggtt tcttactgtt tacactgcaa gaacgccgca aattctgacc
    21841 acatgatgtt aagcctgaac tcacacggtg acttgtgtat catccagaca atcatcaaaa
    21901 tagttatgca taaaatgctc actatgatcg ttttttgcgg catagcgctt gactcctttt
    21961 acagggaggc gctaaccttc cacttgcgag gtaggaagtg tcagggcctc gggttaaaca
    22021 aatgtttgac tcggggcctt tccacatccg gccttcaggt aatccttcca gccgtcagcc
    22081 gaaaggcacc cgcgcgtaat ttacctttta cgtccgttac gtgcaacgga tgacgtggct
    22141 gtttttcctt ccccttcgcc atcctttttc ggagcagttg tgcaaaagtg tgcaaaagtg
    22201 atttttgctg ccactccccg ccaggccgcg ccagttctgg ctttcctgat tttttcgttt
    22261 tcgtcgtcgg atcctttttc agatcttcga tcgtgacgta attttaaaaa tctcttgtta
    22321 ttcagtgcat taaatttttt cgggatcctc cgaaagccgg agtttttgca cacttttgca
    22381 aaaccttgca caaagtgcaa aaccattcag aagggaaaac cccagtaatg gcgcgccctg
    22441 gcggggtgtt ttgcacccat ttcgatttgc aaaatttatc gtacacaaat accggaggcg
    22501 tgtgcggggt agcgccgatt acgtcacttt acgcgttacg tcgcccccga aaatttccgg
    22561 ccatttgcgg cgattttggg gcgcagaaat cgtttttctg atgtcagggg atcgaatcgg
    22621 gctggcgggg ttatctcttc gttacgtggc gttacgcgac gttttacgtg gggattgcgt
    22681 ggggcgtttc gtccatcttt tttttctgct gcggacggtt tttcgtggtg ggtgatgtcc
    22741 ggtgatgaat attttttatt gtccgggaat aaaaaaggcc acattgcgcg gcctgttaaa
    22801 gtgaataatt attcgattat tggtgaatat ttatttttaa gctggcggca ctcctgagcg
    22861 acctggctga actgtgcggc ctgcggagac gtgcccgttt tggggtgggt atgtgatgct
    22921 gtaatatcgg ccagctcttc caccacatcg gcaaggtccg tcagcatggt cagtacatta
    22981 atgccctcgt ttcccagctt caccgtatcg ccaaccagct tctgcgccag cgcaatactg
    23041 tgacggacgc ccttaatctt ttcggtaagg ctgccgccca cggtgtccag tgtgtcgcct
    23101 tccacgtcgc gctcacaggt accggcaacc accatcatgt gcccgcccgt gctgatgctg
    23161 tagttctctt cagtgatatg ggtgaccgcg ccagcctgaa gcgtggatac acccaggacg
    23221 gtggttgtgt cggttgcctg tatggaggtc tcccggctgg tcaggctccg cgtttcctcg
    23281 tctgcgctca ccacgcgctc gcgtgattcc tcctgtatcc gctggtctgt ctggcgctcc
    23341 cagtctccgg cgaccgtcac gcgctgcgat acgccgtcgc gctgttgctg caactgctcg
    23401 cccggcttca cgtcgggcag attgtggccc tctgccatta tctggcggat gaagggctta
    23461 tccgcccggc cttccacaaa tcccacttcc accagggttc cgggtggcgg gaactggaac
    23521 atgccggact cactgcccgc catgggaacg gggaggggaa cggcgttata aacgggcgta
    23581 tccgctgccg ggttgccgtt ctcgtcaagc agctggagat tcaccgcgta gcggggtctg
    23641 aacgggtcgg caatattgcc cgcgctgacg gcttcggtcg gcgcctcaac acgtgccagt
    23701 ttgggcaggt gaagccctga tgccagttca gggaaggcct tttcagcctg gcgacggaaa
    23761 ggggacttct ggaggggggc gccggtggct ttattgcgtg gtgtccacgt gatggtcatg
    23821 ttgtcatctt ccagcatgac catcgtcagg cgctgaccgt tcacctccac acccggtcgc
    23881 agcgtctgaa tcatcgggag ggtcatactg tgcccgcccg ttgatgcctg gctgaactcg
    23941 tgcgggatgg tgacggggcg accggaaaac atcccctgcg cggcggcagc ggccataatg
    24001 ccgccgtccg gtaacggatg ccagatgtaa tccggtacgg agaaaatacg ccccagactg
    24061 gccagcagtt cgtagcccgt gccgctgtgc gtgaaatgcg gaacgggtct gtcagtgtaa
    24121 gccgcgttgt ccggcaggat gatttccagc ccgctggtct cactcagcca ctgtgtgacc
    24181 gtgcgcattg tcgggtgctg aaatgaacag gggaaggggt gatcaaggat ccccgccatt
    24241 tcacgaatca gaagccgcac cgagccgttt tcagcgggct ggctgcgttc aacgtatccg
    24301 gtaaaccagc gcaccaggtc gtttccgtat ccgatatcga gacgaacggg gcgcccggtg
    24361 tagttctgct ctgttctggc cgtgataaat ccgcgtccgc acgcgttcag ctcaagcatg
    24421 acggaacaac tgaccagctg aatggtgtcg ttacccagta acagacgttt agtggggttc
    24481 atggttactc cagcgcatca ttgacgggtt tgaggacctt ttcttcgaac caggtcattt
    24541 tctccggtgt ttcgtcggcc acggtcccgc cttttgcgcc gggcgtctgc ggcgtctgtt
    24601 tacggctgct gccgggggtg gcgcgtgcct ggcgtttttc cggtacgctt cgtctttcgc
    24661 gtaatgtgaa gctgacctgc caggcgaggc ggtcggtctg ctgctgtgcg tctatctgcc
    24721 cggaaaatgt cccttcacga aagttaatgg cggcagcggt ggcgtttgcg atacggtaaa
    24781 ctttcagggc accgtccccg gtggtggcag aggccagctg gaaaatgcgc tgaagggccc
    24841 gctcattgtc aaaggtgatc atcccggtta cgcgaagctc tttggccttg atgccctgct
    24901 cggcgttcgc ggtgctcgat gtctgaccgg actggtcctt ttcctggaac tgcatcgagg
    24961 gcgaaatcac catgttctgc atgaaaatac cctcaccatc aagggcgagc agtgcggtct
    25021 ggctcatgga tcattttccc cagttcggtt aatgagtctg ccgccaggag tgcggccaca
    25081 gtatgtacgg cggtcggtgc cgggatatcc ttcaccatgg ccgccgctgc ggcgcggtga
    25141 tacccccggt actgaaatac ccaggcctgc gccttctgtt ctgccagtgt tctgatcccc
    25201 tcttccagtc cggcaagtgc gccagcccgt ctgctgataa agcggcttat ctggcggcga
    25261 agccctgcgg catccgggcg ttgtgctgcc agctgcctgg cctgtgcggc acgggcggca
    25321 ctgactgcgc tgcgtattgt ggaaacggaa agatgcacag gtgcgggcag ggtgtcggtg
    25381 gtgatcggga tttgcatttt gtcagtgctc agactggcgg cagcctgtgc catgcgctgt
    25441 gcctgcgtga ataccggtac gggcagaacg gcagtcagtg ccgccattga ctgcataaaa
    25501 agatcatggg tgttttcggc aatcatgata atggtcacgt cgccggatgc atggcttccg
    25561 gccattttcc cggccagcca gtttatggcg ttcgggggac ttaaatatac tcccgattct
    25621 tcatatcgtc ccagtccgta agtccacggt gtgacgggga tgacggaaca gctcagcgga
    25681 ggcatgtcgg cgctgacgct gattgttttt cttgtccacg tcatggtgcc tccggtcagc
    25741 ctgcgtgatg atccggcgaa ggggattcag gccagacaat atccggcgcg ttgcccgtgt
    25801 ctgtccggct cagtaatacc cggtattttt tccacagggg aagcagggcg ctttcatcgt
    25861 cggtcgccat atttaaatcc acggcatcct gaaggatgtt tattgtattt gttgcctcct
    25921 gtattaattt ccgtttttta ttctctgcct cgcttacccg gtaagcctgt tctgccgctt
    25981 catccttaac ccatgatgaa ccgtcccagc ggcagaactc cccgtccggt tttatcatgg
    26041 tcacgttttc cggcaacggg cccggctcag taatcagtaa ggcgctcccc gactccagac
    26101 gccagacggt tttaccccgg tgatcttcca tgatgtgcca ggtgttttca tcctcatcaa
    26161 agatggccac gtgtccttca ggaatgtcag gaggggcaat gtccgttgag tgcgcaggca
    26221 gtccggtatg cggagggatc catgcatcgc cttcgccgat aaattcattt gttccggctg
    26281 acaggttgta gattttaatt gtctgcgggt gttcgctcat tttaaaggtc attatgcaag
    26341 cctcacgatg tagttaaatg cgatgttttt aacggtgttt tcggcattac cggtggggtt
    26401 aatggtcagg ccgtgaccat gtgaacccag tgcgatcgtg tgggcgtgtt cgccaatggc
    26461 taccgaatgt gtgtgccccg gaaggtttac tgtcaggttt gctccggagc ccggtccgta
    26521 tgcggtccag tcgccgctgc cttccggtga cgcggtggcg gttctcagtg ctatgtttgc
    26581 agagcctgaa cccgcgcttc ctgttgtttt tgtgccgtaa ttgaaggatg aggtcgtttt
    26641 cgtgccaagg ttagtgtttg agacgctccc tccgtgggtg tgcgatttga tgccgtcctg
    26701 ttcctgtgac aataccgcac gaccgctggc aggttttccc ttgattgtcc agccacgcat
    26761 atccgggata cgaccgtccg gataggcttt tgccagcagg ggatatcgtg ctttatcgaa
    26821 tgtctgtccc gccatgatgg cgtaaccgcc aggcggggta tctgacggcc acggcagggg
    26881 aacacctgcg ggtaacaact cacttgcagg cagtgcgccg atgtcctgtg gtgtgggttt
    26941 gtttttgtcc gtgtaagtgc ggtaccagat ggtttttctt ccccgatcca gtatcacccc
    27001 ggtaaattgt gcccggtgtt cttttgtggc cggatctgaa tcttccagtc ctgtggtgat
    27061 cctgactatt ccgccgatga gttcgccaaa cacttcaatc aggcagccac ccaggcagat
    27121 atcgccatac cccgtgtcag taagcacgct gtgctgtgac agtgaaatat tggcccttaa
    27181 tacccagctc cccgttctga atgcaccgcg ctcattcagc cattccacga actgttctgt
    27241 tgtccagcgt tgccctgctt tacccacgta tccgtcagca tcataaaccc ggggagcacc
    27301 gatgttgata ataaacaatt ccttgttggg gatgtcctca ccattgcggt ttttcgcaag
    27361 ccgaccatcg gcgttgtcca tggcgatctt tatcgctttt ggcgtggctg cctgtgtttc
    27421 tgatgtactg gtggtggcgc tgcttaactg cgtgaagccc ttttctttca gcgttgcgtc
    27481 cgggtggttg cgtgatttct cgtgtttttt cagggcgtcc agactgcggc gctcttccgg
    27541 ggttcctctg gggcgcaggt cagtcacgtt gccgtcttca tcaatttcag cgatggcgca
    27601 aaaatattgc tgaatgccgt ccctcggata cgttttccgt aaacgttcct ccgtaacgtc
    27661 cgggtcggct gttatgattt cgtgctctgt cgtccattca ctggtgagcg taccggtcca
    27721 ccacacattc agccagatac gggacgggaa ggcgggcgtg accattggtg tgtcttcggt
    27781 cagctctgcg cgtagcccct ccacgtaacc cacccctttg ttgactgtcc aggtttcgcc
    27841 tttcttaacc agccacccgt catcaaaaaa agttgccttc ccgtagatat cgacgttttc
    27901 acggcgctgg cgttcgtcca tggatgccag gcgtgccgtg aagtctatct gccaggtctg
    27961 tgccggggtg gtgatacctg tggcttcctt cgcaccgctg tactccatca gcatggaacg
    28021 gaccagcaca ttaccttcgt taccgttgtg ggttctgatt tttttctgtg tgggggcgtg
    28081 aacaatcatg gccagcgtgc tggttgcttt gctgaccagt cccacccagt taaattcaaa
    28141 atcacccagc tctgccccca gggtgaccga ataaaccacg gcattctcat tcaccacgcc
    28201 ggttttgcca acggcctggc ggtgcacaat ctgatcttca ggcggcaggg tttcgtcggg
    28261 ggataccggc gtttccgggt tgaggtccgg gacgtttgca aaaataaact catccagcac
    28321 aacgggggtg ccttccgccg cctgccgtgc cttgagccgg ataaagtcgc tggtaattac
    28381 agatttcgcc ataaagtcat actccttcag tcattcactc tgccctggcc gtccagcaga
    28441 tatactcccc ttcgtatgcg ccataatgga tgatgaaggg gttgagggtt tccacctcaa
    28501 aacggtaacg gcggcatgtc ctgccatatt tgcggatgat ctccagaaga agatcgctgt
    28561 tgtcggctat ctggctgtct gtcacccgca ccaggatcac atcccagtcc acgccgggct
    28621 ggcgctcgag taactccacg tagccgatgc ccagccgttc aaaaatggcg ataaagcccg
    28681 ccacctcgcc agcctcctgt gcattgatga aggcgtaact gatgcggcgg cggtagatct
    28741 ccgtgggttc tcctttaaag cgagtgatgt cccgctccca tgccagtaaa tcaagtacgg
    28801 gctctgcaca tgtcatgggg tcaaactggc gcacgggcca ggtgatccat tcccatacgc
    28861 gggaccagaa cgcctgcgcg gcccgcagca gcttgtcggg ttcccccctg tccatccagg
    28921 agggcagttt cagcgagcga agccgctgca aaaagtcatt caatgaccac ctccactgtc
    28981 agggattcga gccgtggcac ggccagatcg ctgatgatgt cttccagtga aaactgaacc
    29041 gaagccacca ccgggaaggt ctgatgaatt tccttgccca gcgtggaaaa cgaaaagcgc
    29101 gaatagggcc agactttttt cacattaaag cgggcgttct cccgaaaagc gcagcgtatc
    29161 aggtcgctga tatcctgttt cagcccttcc gcgccgtccg gtggcagggt ggttgagctt
    29221 ctgatgtata cggtgacgtc cagacggtga cgcgtctccg gcatggcgaa acactgcaaa
    29281 tcatccccgt gtccgtggtg cccctcttcg ttgatgtagc ggttgactgc ttcaatgaaa
    29341 ggggcagagg cctcgccgct gtccagtaac aggtaggcgt tggccgttcc cggtcctctc
    29401 ggggcgtcgt gctgaaagta gatccggtcg atgctcaggc ctgccacgct ggcgataagt
    29461 ccccggtaga cggcgtccgt gtggtaactg ctcacgaggt taaactggtt gcgtattctg
    29521 tcgcgcagtt cgtcgtcgct ttcctcatcg gcacccggta cggtcagcca gtcttcccgg
    29581 ttcaccacgg atgcaatgcc gggaatggca acgggaagga tacggaagta ccccggcgcg
    29641 aggttgtgcc cgctgccttc ggtggtggct tccacgggaa cggatgcgcc gcttttaccg
    29701 ctgctgataa gcgtttcctc cgtgacggcc atgctgtata tcacgccgtt aattctggcg
    29761 gtctggacga tggtcccttc ggggatgaga acatcatcat ttgccctggt tttggcaaac
    29821 cagatcacgc cacgggcctt actggccggt tttggggtga tgttgacgtc gtgcgcatga
    29881 agcagaagaa gtgaccggtc agccgtggcg acaaacatac cgggcagcac aacgtcaacc
    29941 agcgcatcac gcagccactg aacgggtcgt atgacaatgg tcagaatgag ccgccagaag
    30001 ggactcatgg gcgaggtgtt ggtgatgagt ccggttttct gtacatcctc atgaaagagg
    30061 gtggcgatct cttcttctgt ttccggcata ccgctttcac ggaatacctg attaaaatca
    30121 acgtccggtt tccctttcat agttcaccct ggtgtttatg cgtccaaaat cctgtgtctc
    30181 tgccgtcaca tgcagcctgc ccggtgcctc ttcgttaatg aacaccgtcc ccggaaccag
    30241 ccgttcatca tcctcaacca ggaggataag ccgggtaatg atgtcgctgc gcagcgtcgg
    30301 gctgcgttcg ccgataagca gggcggcgat cccgctttcg ataatgctgt gcaccacgtc
    30361 ctggccgatg ctgacccggt tatcacatcg ctgtggctcg ttgccgctgt tcagcgtaaa
    30421 actgccgtca gtgataagca ggtcgatgta aagcgatggc gtgttcatcc cgcgtaaagc
    30481 tccgtccatt catccagttg ccccggcgtc atcgggttct gcgtgttgat tgtcagattg
    30541 ccgaacgtcc ggctgttgtc gatacgggtt gaggtgtccg tgcgtatctg gttgcgtatg
    30601 cctccgggtg gcagctctgc gctgatgcgg ttgcctgcca gtgccgggac ggcggtttct
    30661 gcaatctgcg gaggtacggt gctgcccgtt gcgccccgcg tcatggtgtt cacctgctcc
    30721 gtgggcttca tgtcgatact gataccgggg attttgttca gcttttccgc aagccagttc
    30781 cacgtccggt taaacgagtt tttaatgacg ttccataagt ccccgaagac gttaccgatg
    30841 atccccgcca ttttccggaa ggattcgacc ggggcagtcg ggtcaaatgc agccaccacg
    30901 tcctgccacc ctctgatgac gatggcgaag atgtcgacca gggttctgat cacgctgata
    30961 accacctcca gcgcggggga tatcaggctt accgctgcgg ccaccacgcg accgcatgtt
    31021 tccccggcac gggtgacgct gttcatctgt cccgtggtca gctgaaccgg tgtgaacaga
    31081 tcagtgaagg cgccgaacag ggtttttatg ccctgccata ccggggccag cgcacgggca
    31141 atttcaccga acgcggcagt caccgggctc atggcgtcca gtgcgccggt aaaaccatca
    31201 ataaagccgc gaataaaggc tttgacgggc tgccagaact taatggccac caccaccaga
    31261 gcggcgatgg cggcggcaac caggaggacc ggggcgctca tggtcagaaa cgacacgccc
    31321 gccagacgcg cggcaagact ggtggcaatg atgacgctgc gtacttttgt gagtgctagc
    31381 gtgtacagac tccagactgc cctggcgccc tttatgatgg ccttatggcc ttccatgatg
    31441 aagctgaaca cacccagcac gatgttcgtg accgcaccgg ctgcgcccag cgccagcatg
    31501 gtcagcgtga tgtaacccag ccagcgggcg atgttcggga acatgttcag ccagcgcacg
    31561 aacgtcgcgc cgatatcggc aaaacggcca gccagtggtg cgagaatggg gtagagggcg
    31621 ttaccgatgg cggcccggat gttgtagaag gaggccacaa ggcggtccca cgggtcggtc
    31681 atggcttccg ccatttccgt ggcacgcttc atgccgtcat tacgccccag cgctgatatg
    31741 ctccggttaa gcatttcctg ctgcccccag agttttttaa tcacgtccgc gccactgccg
    31801 aaggcatcat ccagcgcctg ttgtgccgcc acgttgccct cgatgttcct gccgaacctc
    31861 gcctgcagac ggtcgaggat gtcacccatg ggtaaaatct tcccggtggc atcgacaaaa
    31921 ctcatcccca ttttttgtgc ggcagcgggg gcactgcgca ggaactcctc atagatgcct
    31981 gatgcctcgg tacccatggt tcgtgacagg gtgcccagta tggcaaactg ctctgccatg
    32041 ctgacgccaa aatccgcgcc cgcgtttttg gtgccctcaa taagctcctg catggtgctc
    32101 atttccacgc cgaactgctg aaccatccac gcggttttac ctgccagatc ttcagcaaac
    32161 cccacggcac ccatggcaga aaactcactg cggaaacggg cacccatggc ggcaaggtag
    32221 ccgctcgcct cttcgctgct gacgcgggca gccacagcca gggtgctggt tgctgccgtc
    32281 acacggggta aatccccgtc gctcagcccg tccagtgcgc cgcgaatatg ccatgcggaa
    32341 cggatgatct ccgtggagga ctgaccgaat gcggtcccca gtttcatggc ctgcgtggtg
    32401 agggcggtca gcgcctgcgc cccgacgcct ttggcctcaa gctcgcccag cgccttctgc
    32461 atctcccacg ccggatccat cgcggcgcgg ataccttcag ccacgcccca cagtccggcc
    32521 acacccacac cgatacgcgc gaacgcgccc tgcgagcgtt cggcgaatcc cgtcacggat
    32581 tcctgaacct gccgcagcgg gcgggtaagc tggtctgtca gattgataat gaaatccagc
    32641 tggctggcca tgtcaccttc cgttaaatgc gatcgctata ccttcagcgg tcttgttggc
    32701 gcgggtacgg gcaaaatatt catccagcca gatggcacgg gcgatgctct cttcgtcgtc
    32761 cggctctccg gggaggtagt gacgccgcaa tgccagatac tgctcaaggg gattgcggcg
    32821 gatgctttcg acccgcgcgg tcagtttttt aactcaatct ccagtttcgg ggagtaaacc
    32881 tcgttaatct tctcgacgat ctgcatctca cagcccggat aacgcgcaat caggtcagcc
    32941 agtgcgtcgc ggctggcagg cgtcacgatg cggttaagat aggtgctcat cggcgccacc
    33001 ttgttggtca tcgtgatttc gttgatgagg ttgttaaatg ccgttttgtt cggttcaaag
    33061 ctcagggtta cgcccgcaac ggacaggatg atggtgccgg tgttcttcgt ggtcattgca
    33121 gtttctccgt gtttcgtatg gtttccacca ggctgttatg cctggcggcg cattcggtgt
    33181 agcggctcgt gagggttcgc agtgccgcat caaaatcagc gccagtcggg cctgccagtc
    33241 tcggcagtgt cttcgggcat tccgtcagct ggttttcctg ccagggtacg ttcagcggct
    33301 tcagcacggg cgttgaacag ccggacgtaa tcatcagtag cgcacacatt gcggaatacc
    33361 gggcggacgg tttccgtgtg aataacgcgt tcagtgtgaa tttcattgcc ctttaacgcc
    33421 tccagccgtt tttccagtat gcgggcggac tgctgcccgg cttctgtcac cgcacgtcgc
    33481 gtggcttcca tggcttcctg ttcggcaagc gccaccctgg cgtcatgcca gtcatgggcc
    33541 agccagcctg cccacatggc ggcaaagagg atcaccaggc agcccgccag ctgtttcatc
    33601 atttcacccc gttgtgctca aggctgaaat gattgccgtc tgcccggctg aagcgtccgc
    33661 cccatgtgcc gccgatggat tcccagtatt cccccagcgg cctgtaggcg tcggtgtcgg
    33721 tctggtattc gccgttaata aacaggttga agtccaccgc gaggcgcagg gtgtgaaggc
    33781 tgttgcggat gccttttccg gttttcgcat tcagtgctgc ctgctccggc gtgcggtatg
    33841 cctcaccgaa ggtcaggcga tagccgtgct cctgcgccca gtgaataagg tcggcaatca
    33901 tgacggcaaa cagctgttgt ttttcgctca gtttcatcag tcactcctct gttccgtccc
    33961 cgtaaggcgc gcctgtgcca cgctgaaata gtgggcgttc agttcaatac ccgtaaaacg
    34021 gcagccccgg cgaagggcgg ccacgcccgt ggtgccggac cccataaacg gatcgaggat
    34081 cagccctccg ggttcacaca cgcgcacaag ctcatccatc aacgattccg gtttacctgt
    34141 cgggtgctgt ttctcctgtg ggcgtgtgtg gtgcgtggtg cagccgtcga acggcccgga
    34201 aggggatttg cccagcgcgc ctttactgcc ccagaccaca tattcgcact ggtgacgaaa
    34261 ataaccggta tgcggtgttc ttgaggcccg tcccttgttc catgccacga tcccccgcca
    34321 ggtaaagccg gcggcctgga atgcgtccgt cagcgcgggc aactgccgcc agtcggtgaa
    34381 gaccagggcg taaccaccgg gtcgtaccgt gcgccatgcc tgtgtcatcc acagatgcgt
    34441 ccagtattcc catgaacggg cgtccatgtt ctcgccttcg aagccttcaa agcggtgaag
    34501 atcttcatcg ttgatgtatt tggcgtggct gccctggcat gtccgccccg ccttgtgtgt
    34561 ggcacctgaa ctgtatggcg ggtctgtgat gagcgcgtcc acactgtttt ccggcagcat
    34621 ccgcagaacg tcatgcgcct gcgcgtgata caggctcagg gaaggcgaaa tgtaaggctt
    34681 atgggtcatt tctgcgttcc ttctgcttca ggtagcggtc tgccagacgg caaaagcatt
    34741 cttcaataaa ggcactgccc ataatgccca gcgcgcacgc cagaccgacc acaaccagtt
    34801 ccggcatatc cggaaattta aacagcggaa tggcggcaac cggcgccacg gcagagccca
    34861 taatcattcg cccgataagc agacgggctg tgatgcgatc gcggctcacc atgagacgcc
    34921 ccagcccgat ggcggcaccg atgataatca gttttgccag gagtgaagtc tgaccgtccg
    34981 gcatggcatc accccttcag atcgcgggtg tcacgggctg acaggtaggg cacgccgtca
    35041 atgctgataa agtccgggct tgtgaccatg aacttgattt ttttcgtggt ctttccggct
    35101 tcgcctgcgc tgatgttcac cacgtccgac aacatgggga cgcagccgaa tacctcgatt
    35161 ttttcctcct cgctgccgtt gttggcataa aagagaaaat ccttttcagg aatggcacgc
    35221 catgaccccg cagagcgggc cacagcacca aactttttaa agttctgcgc gtcgacttcc
    35281 acctccacat cggcagaaac ggaaccacgg gtataaccgt tcggcacgcc gcgtgtctgt
    35341 gccacggcgc tgttgtcggt gatggtgacg gtggcgcttt ccacgtggat catgatcccg
    35401 tcgtagttga tatcaaacgc gccgccgctg atacgttcat ttccggccat gtcagatctc
    35461 cagtgctgtg tcgagttcaa tacttacgcc aatggctttt gcgctctcgt aggggcgtac
    35521 cacaatgtaa atctgcacgt gtgtactgct ggtccagacg atggtgatat ccccgtcctg
    35581 tggcggttta acctcgccgg ggaacggtac gccgttaatc tctgtggcga gcgccatttc
    35641 acgcagcgtg cggctgaaat atgtctggct ggcggcaatg ctgcccggtg tgctgttgag
    35701 cgcgcgatcc gcaaggcggg caatggcacg cagacgtaca cggcgggaga ccttatccac
    35761 cacgcgcacg ttttcgatga cctggtaatc gcccccctcc acatcaaggg tgcgtccgtc
    35821 tgaccagtag agcccgtcga aatcgtgata ccacatgggc acgctgaagc gtttgccttc
    35881 cagcgagcgt agcgtggcca gctcaagggg ctgtttcttg ccgtcctgcg ggatctcatc
    35941 gttacccatc gccaccagag caccggttgc cacgcgtgcc gggctgtccg caacggtgac
    36001 agaaggatta cagagacggc ccgccagtac gccgggctca tttccccata agcggggaac
    36061 caccatcacg ccaggtgctg ccacgccgtc ctgcatggca ctgatgcggg tgacgtatgc
    36121 cgcccatgcc tcatccctgc ccggtccggc cacgcacagc atgaaccaga caaaacggcc
    36181 ataagcggac tgaagcaggg cgcgggtttc ggtggcgcgg ttaacggcat ctttagccgt
    36241 gatgtcaatg gcaatcacca cgccttcggc agaagcaatg gtctgcgctt ttttcaccgc
    36301 gtccacccat gcggcgttag cgtcgccgcc ctccggtgtc gggggtttgg gttcagacag
    36361 aacgtgaaca taggcaaacc agttctggcc accgttggcg attgcggcgg taagcgtgga
    36421 tttaagcagg ctttcatccg gtcccagcac gtcgtccagg tcggtctcag gggagacgga
    36481 aagggtgtta cctgtgttct ttttgccata gcccacaaaa agcagcgtgc gctccacttc
    36541 acgggtggca ccgttgaaac ggttctgatg gcttacggtt acattcggcc agctcacggt
    36601 gtgactcctt atctgatatc ctgtgcgtca acgtccaggc cgtagcccat tgcgttcatc
    36661 tggcgtgcca ggatgtgtga aaattcttcg tttgttaccc ccagaaagac gcgcgacgga
    36721 agggtaatgg tccatgtcct ggccttctgc tgacgtggtc cggcgataat ctgatcctcc
    36781 ataacggtga tcagcaaccc ggcctgcgcc cgcgaaagcg ttaacaggta tttcatggat
    36841 gctttccgcc agcgcccctt ctgacgtacc ttaaagccca gctcccgcag tcgttttgcc
    36901 tgtcggcggg tggcggtctg cccgctctgg tcctgaccgc tgaagctgtc agcactgacc
    36961 cggatttctg cgccgttgtg gtgaacccag cccacggcac cggcactgat gcggctcctg
    37021 ccactggtgt gattgccgtt acggaaataa acctttacgg catcctgtcc gggcatctca
    37081 cggaccgcca gaagacgcgg cagctttctg aacattttcc ccttgccgcg tttccggggt
    37141 gcccatgctg tgccgtccgg cgcggtctga tcgcgctgat ggcgtctggc tgcggcgata
    37201 atgccgtgcc gggcaatacg ccataacagg cgacggcgtt ttttcggggg cagatccagc
    37261 gactgaagca tcgcctgcgc ttctgccagc tgctgcgggt tcagctggcc gcttatcatt
    37321 cgcggctcct gctgacaaac agatccatgg tttccgccgt ccagatttca gggttaacca
    37381 gtgtccagcg tttccccctg tacggaacgg ggccgtcctc ctgcacccgg atgatcagcg
    37441 ggtcagccag tcccaccgtg atgtcgagaa ggcacgagtc ctcatcgtta aactgcgggt
    37501 cgacgttcac atcctccggg cggaattcct ccctcagcgg gtccgcgtgc tcttccagcc
    37561 aggccatgac cagcgcgtaa accagccccg gtgagtattt gcggaacggg aagttatccc
    37621 atgcgatcac gccggtgtac gtcatcacgc ccatgcggat ctggtcgtta cccagtgcct
    37681 tctgacttcg gataagttgc gcgtcttcct gactgctcat gaagaactgc atggcgtcag
    37741 acggcagatt ttcggtaatg aaggcggtca ggctttccag ctggttcata tgatgtgcac
    37801 tcccgcgcgt ctgtgcccca gcatgttacg catgaccagc gaggcttcag ccagtaaggt
    37861 ggcgcgtgtg tcctcgcttt cctgccccgg ataggtttca cggcgaccaa tggtggcgaa
    37921 ctcgcccata agatcggcct tggcacgggc ataaacggcc tttttgtact gtgcgaccag
    37981 ctgggtttca tcgcccagcc tggcccccgg tacgtctgcg gcgcgttcat gcccctgcgc
    38041 cttccagtgc accacaaccg gcgccagcgt ggtgttcacc tcggccactg cggcaagcag
    38101 cgccatggcc gccgtgtcgt cgggtaaatc agccggaagc gtgcgtgacc cctgaagctc
    38161 agccagatca aggtccggcc agaacggctc gccgtttgtc accgtctgcg gtgtggattt
    38221 cagcggttta ccgctgacgc tgaagctcgg ttcgctcatg acgtgcctca tatgaataaa
    38281 aaggcgggct gtccgggatt ccacggccat acagtttccg tcactgatgc ctcctcccgc
    38341 gcccgcccgg ccctgcgggt agtcgtttac gctgcattaa gggcgctgat gcgtgccctg
    38401 atgcgcgccc tgatactttt tacgccagcg gtacggctca gtgctgccgc ctgtgccagg
    38461 tatgcgtctg ccttttccag tacggcagta tcattaatcg cggtggctct cggtttgccc
    38521 gcctcgtcgc gcagaagata cagcccggca aatctgaacc acttcgcgcg gatttcctca
    38581 tgcagccgcc acgaggtggt gacgttcctg aaggtgcgcg aaaaatacgg ttctatgctg
    38641 tgtccggcct cgctgttgtc agtggcccac tgcagcaccg tgtctgccac gaaggcgggc
    38701 agggtgctgc ggaagttgtc cggtgtggcc tggccttcac tgatggcaat gtctgcccag
    38761 tcaagggcgc gactcaggtc gcccgtgtcg aacagccaga tgatgcagta caccagtacc
    38821 ggacaggcgt ggcgctttcc gtcagcgata tagcgctccg cgacgggcag ccagcgcggc
    38881 aggagaatgt cgcgcttgaa cgccagccgc tcttcgcgga ccggaagact gcgcagccgt
    38941 gccacatcgc gatcaagctc cgcgcgtaac aggtggaagc tgaccgggga ggccgtcagc
    39001 gcctcccggc gttcgagtgc ctcctgtgtg cggattttcg cccggtgccg ctggcagggt
    39061 gtcattgcgc cgccctccgt cctcagccgc cgacgcccgg tacggcttct gcgcccttga
    39121 tggtcagttt gtcgtaggcc gcgtaaattt cgtcgtgctc cacggcgtaa ccttccatgc
    39181 gcagatagtt gttttcgaaa cgcttgcgat cgtcgttcca ctctgcacgg cgctgacggg
    39241 taccgcgctg cgtgtagatg tgaaggttcg ccagcgtggt gacaatcaga cggccttccg
    39301 gcataaacgg cggggtgtat gccgtcagtc cggcgatctg gcgaccgata agctgtgcgg
    39361 ccactttttc ggtgggggcg tcgatggcgt taagcagcgt caccttgtcc tgtgccacaa
    39421 gatcgggggc taccagtacc accagatccg gatgaccacg atacgactca tcaatgaggg
    39481 tgtgcacgag atctgccacg gctgcatcca gcccgataaa gtcgccaccc ttgcccagcg
    39541 tcgggccagc ctccacgatc tgttttgcgc ttcgggcttt gacgatggca tgccagccgg
    39601 tgttgacgtc ctcgccgttt ttgttggtta ccgggtcagt attttcggcc acgctggtac
    39661 cgttaaaggc gatgcgcagg atatccagcg cgatctgtct gttaacaaaa ttctgaatgc
    39721 ggttaaagaa ctcgttttca cttcctgcgt tcgcccaggt gaccagtgtg gtgtacagga
    39781 ggtgggcgcc ggaatcggtt tcagccagct tgtattcatt cccggtaaca cccagctgaa
    39841 tggtgaagcg tccgtccttt ttacgtccgg tgtggaggcc cggcgtgccg gtggtgacca
    39901 cctggcctgt cacctgttca acatccagca cgttcggcag ttttgataaa aactcgtcgg
    39961 agtgcatgat ggcgtcacgt agcgcggtct ccttcgggtc agacagcgca aaatagcgtg
    40021 tgacgtcgtc aaccccgttt gccgcaccca gcccggcagc gtacttacga agcagtgatt
    40081 cggttttctg gttcagttgc atgttgcgta tccttaaaca aagtcaaacg gtttttcgct
    40141 ttctgccggt gtgccagccg ggcgtggcgt gatgccggtt gctgacagct tcgtggtcag
    40201 gtcgttaagt ttttcggcaa tggctttgat ttcttctttg tcggtgtcgg cctggcgacg
    40261 ggcggcaaag cggcggtaac gttcgccttt ttccggtgtg atggtgctga aggctttgat
    40321 ggcgtcttcc agcttcgctt ttgcagcggt aaaggcttcg gccttaacct cgtcttccgg
    40381 ggcggctttc acttcttctg ccagctctgc cacctctgcg gcggcgtcgg cgatttcggc
    40441 ggcgatttcc gcgacttcgg cttttgcttc ttccggcgtg tctgctgtgc cctcgccagt
    40501 ggcagcggcg ctgccgcctt ccaccagggt gatcagcttt tcaagcagtg ctttaatttc
    40561 gtccattttt gcgtcctcgc ctgtatcagg cttactggta tcggtgttgt cggtctgtcc
    40621 tgcgctgaac aggcgtttca ggaatgattc aggtttacgg ggggcagggg tggtgtccgg
    40681 tgacaggctg cccagcgtga acgtctggca gttcgcccgc tgtgccgtgt attcctcgtt
    40741 gttcagggtg aagcgtattt tttccgtccc cagactggcg gggatatccg tcaccgccac
    40801 acccatgaga tagtcgcgtc cgcttttggc aaagttcggt attaattcgg ctgatgtgaa
    40861 gagtttttgc cccagcctgt tggcttcaat caggtactga ttagggacca gtcgcgcgta
    40921 aagacgggtt ttaccgtcct cgcgggtttc cactttcagc gcgtccactt cgcccagatt
    40981 gaaggtgtac tggcggtaac tggtgtcgtt ctcatggcag ggccacagca tcgccgtgta
    41041 ggtgttgcgg gaataggttt cagcggcatc aatcagccac tgcggatcaa tttcgcgacc
    41101 gtcagcggtc ggccctgatg tggcgatgca tgtccagtcg gtcatcaacg ttggctgcgg
    41161 catcgtggcc tcttttacgt gaaaatctga agaactccgg tgagtgtcgg gatttttaca
    41221 gcggggggct actggctgaa ttcggctgtc ttcggataac gcgggatagc cgaacacagc
    41281 cgaaattttc tgcctgaatg cgggccggat gcgccgccat aatgggcgca tggctaaata
    41341 ttctgacgaa atcaaagagg cggcgcgatc gctttacctg aagcggtggc acccccgcga
    41401 tattgcggcg gaactcggta ttccgccgcg cacggtttac cactggtgtg atgtggggca
    41461 gtgggctacc ctgctgcccg ctgaatccgt tgaggacgtg atcgcccggc gtattgatgt
    41521 gctgaccgga cgggagggca agacagagtg tgaacttgcc gaactgcgtg aacttatcgc
    41581 gcagcacgtg aagatcatcg cccagaaaaa caaacacgct gaaaggatgg cggaaattgc
    41641 cgccctgcgt ccggcagggt atgaggacgg cacggctgcg gggacctgcg gcgggggaga
    41701 agggaaaaag cggcgctacc ggaaaaacga cgtttccgga ctgactgctg aaatgctgga
    41761 tgaatgggcg cgtaaaaaac tttatgccta ccagctgcac tgccgcgaga accggcaccg
    41821 tgagcgccgt ttcatcctca agagccgcca gatcggcatg acctggtatt ttgcatggga
    41881 ggcgttcgaa gatgcggtgg tgactggtga taaccagatt ttcttttctg cgtcacgggt
    41941 acaggcggaa atctttcgtg aatatattat taacttcgcc ctgcagttcg ggatcacgct
    42001 cacgggtaag catatccgtc tgagcaatgg tgcaatgctg cgtttcctgt ccaccaatgc
    42061 cagtaccgcc cagggattta acggccacct ctacggcgat gaggtgttct ggatcccaaa
    42121 attcacgcga ctgcatgaag tggcctcggc aatggcgacc cacgacaaat accgcacgac
    42181 gtatttttcc acacccagcg ccaaaaccca ccaggcttat ctggtctgga atggtgacga
    42241 ctggcgcggg gatgatccgg cgcgccgtgc tgttgagttt ccgaaagaaa gcgcgatgcg
    42301 tgtgggctgt gaatgccctg acgggatctg gcgctacatt atccggctgg aagaggcggt
    42361 ggcgggtggc ctgtctgcac gggttgatat tgaacgtatc aggaaccgct acaacccgac
    42421 cacctatgcg atgctgtacg gctgcgagtt tgttgacagc aaggatgcgg tatttaagtt
    42481 ctccgaactg gtcaggtgtg aggtggagat ggagacgtgg ggtgactacg atcccacggc
    42541 ggcacgtccg ttcggcaatc gtgaggtgtg ggcgggcttt gacccgtcaa ggtccgggga
    42601 taattccacg tttgtgattg tggccccgcc tgtccatgag ggcgagcggt tccgtgtgct
    42661 cgctgtctgg cagtggcagg gatttaactt cacctggcag gccgatcaga tccgcgaact
    42721 gatgcagcgc tttaacatta cctatatcgg catagatatc accggtatcg ggaaaggggt
    42781 gtttgatatc gtcagtcgct ttgcgccacg tgaggctaac gccattcttt acagcgtgga
    42841 gagcaaaaac cgcctggtga tgaaaatgat agacgtggtg gcccataagc gtatcgagtg
    42901 ggcgaaagac gccattgatg aggccagccg tgaacgcacg gagatccccg cgtcgttcat
    42961 gtctatacgc cgcaccacca caaaaagcgg taacgcgctg acgttcgtgg cggaacggtc
    43021 ggatgttacg gggcatgcgg atgtgttttt tgccatttcg cacgccgtga ttaatgagcc
    43081 tgtagattat gactatgaac gcccgtccac atgggccttc gggaaagccg catgagcaag
    43141 aaacgcagaa acaaaacgtc acgcgtgacg aaaaaatcat tcacgcccgc acgtggcagc
    43201 acaatcacct ttggccagcc ggaaccggtg ctgaccaccg gcacggacta ccataacatc
    43261 tggtatgaca acgaacacaa ccactggcag ctgccgatta accgcctggc actggcgcag
    43321 ctcccgaacc ttaacgccca gcacggtggg gtgctgtatg cccgccgtaa catggtggcg
    43381 gggggctata ccggtggtgg tctcacaacg gaccagatgg aacaggcggt atttgactac
    43441 ctgttattcg gcgatctcgc cctgctcaag gtgcgtaacg tattcggcga cgtcgtggct
    43501 cttgagccgc ttccgtcgct gtatctccgg cgccgcaaaa ccggtgagtt cgtggtgctt
    43561 caggaaggcc ccgcgctggt ctatacgccg gaagatatta tctttctgcg gatgtatgat
    43621 ccgcgtcagc aaatttacgg gctgccggat tatatcggtg gcattcactc ggcgctgctg
    43681 aacagcgagg ccagcctttt tcgtcgccgt tactacaaca acggggcgca tatggggttt
    43741 ttgctgtata cgaacgaccc tgggatcacc acggagatgg aggatgagat ccgcgagaag
    43801 gtggcggagt ctaaggggct gggaaatttc cgcaatctgt acatcaacat ccccaaaggt
    43861 tcgccggatg gagtgaaaat tattccgatt ggtgaggtga gcgccaagga tgaattttca
    43921 aacatcaagg ggatcagcgc ccaggatgtg tttacggcac accgctttcc tgccggtctt
    43981 gcggggatca tccccacgaa cggagccacc atccctaacc cggaaacgac gcgtgaaacg
    44041 taccgcaagg atgaggtgat accgcttcag cgtaaaatca tgaacgccgt gaacagcgat
    44101 cccgatatac cggaacacct ttatctttgt tttgatgtgg aagccgggca tgtggcggaa
    44161 gaaaaagcgg taccggtcgg gtaaaatgtc ctcccctggg tacgtgttat cgggaggtaa
    44221 gatgcggatt tttaaagccc ggtgtcctga ctgtggcgca cctgctgtca tccggaagac
    44281 agactggaaa acccctgaac ttgcggatct gtactgtgca tgcagtgatg tcgaatgcgg
    44341 gcataccttc gtctgtacga tgaccttttc ccataccctc agccccggcg cccgctccgg
    44401 tcgcaatatg gtgaagtttc ttgttgatgc catgcgcccg gaagatcgcc ggtatgcact
    44461 tgaacttctg tcgcggcgtt cctgacgcgc ttcgcttgtc tgctgcttcg ccggggcaaa
    44521 aagaattttt gccccgactc accagctctc ttaacggaat gcggcgggcg cttttctttg
    44581 taaattagtg tgcccagtcc catgctacct gtccgcaggg atctccttat ttgtgtggtt
    44641 atgtatctgt tgtagatatt tcatcttccg aatgatgtcg gctttgactt cagggttgtc
    44701 aacgttcagg gtcgcattac cgtttgcgtc aacaataagt cggtattttg ctgtttgctc
    44761 cagatttgta tcaacatgtg atgctaccgc atccgtgtat cggcgtagta ctttccaaat
    44821 aagtgttggc atatctcttc ctcgtggtta gtgactaact tttaccacat ctcatctttt
    44881 tatatgtttt cctttcggtc acgagagcaa ccagataacc gcaacggttg ccgcgaaaat
    44941 tctggcgata aagcctgctg tgaagtggcg aaacgggttt tcccacgtca ggaatgccat
    45001 taaagcaatc catgcaatgg taaaaatcag tgcgccctta acggcgataa acagttcgtt
    45061 cattgtggtg tcctcctttg tgtgtcaggc gaataaattg cgttgcggtg aaaaaaatca
    45121 ggtgcgccag cgcagcctta ctgctgacgg cgttcccgga gttcggtcag tgctgcaagt
    45181 aaattctccc accacgtttc ttccacgacc gggttttgtc gtgaaagtag ctgctgcgcc
    45241 gactcaatca tcttcttcag gtctttgtcg ctgatccttc ggggattgtt catggtgtgt
    45301 tccttctgtt ggcggttgaa tgcacatagc gggcaattgc cgctttgcgg ttaattgtga
    45361 ccagtgttct ggtgagttct gcgttgtgtt tgtgtgcctg ctcttcgtgg tactgccgca
    45421 tgatttttcc ggcaggcgtg agcggctccg gctccccgtg cggacggcgc ggacgaacgg
    45481 cgacaagcca gctgcggtta gtgctgtccg gtcgtccgtt gagtatgtcg gtgatgatgg
    45541 ccgtggtggt gcgatcggga ctgacgactg tgatttccag ctcatcgccg ggttgtggtc
    45601 ccgtggcaat gttgttcagg gtcagtaagg gcgtgcgctg gcgcatcatt tcgtcaaagc
    45661 gctggcgctg catggcgcaa taaaagcggg tcattgtggc gcctccgtat tcggtgcggg
    45721 ggtgatatca atgcggcggg tgctgtcgtt ccagtgcgcg gtgcgcccgt cagccaggcg
    45781 cacgcgggca ccgtgccgga cggaaacgac ggcgctttca tgcagctgca cgcccagcgc
    45841 atcaagctcg cgggtgattt gtgcggtgat gtgtggctca gcggtcagtg cgacactcac
    45901 gcgaacgccg tcagcgggcg cactgatgcc gccgtttttc acggtttccg tgtcaggtgg
    45961 cgggtttttc agctgcctgg tgagttgttt tcgctctgtg ggggtcagtg accttaacca
    46021 ttcacccaaa tcaccttcag gcagggtaag tcgctgctcc ctgcgttcgt ccggctgcct
    46081 ttctggcggg tcgtttatgg tcagattctg gtcaggggga cagttattgc cacgagtcca
    46141 aggtgcggca aagccgcact caacgtcaaa accctcgtcc gtgcggtctt cgttttccgg
    46201 cttacgtctg accagttccc aggtgtccgg gtgggtgcat atggtgtagc gctcccccag
    46261 tgacggggcg cggacgccgt aaatgcgttg cgggaattcg ccgtagtcgt tggcctcttc
    46321 ggctgtttcg tatgcggtgc ggaccaggta cgtgttgcgg ggaatgagca caccaccctg
    46381 gcgggtgata taggtggcaa aacatcccac gtcggcggcg gccagtatgg cgtcaatgtc
    46441 gtcatggtcc agacgctgcg gggcagccgg gttacgggcc atctgcccgg caaggcggcg
    46501 gagttcgcgc catacctggc ggggcgggat accgaagaac tggaactggc ggatgcggtg
    46561 aagggccgcc cagccgatgg cgttctccac ggtttcggcc atggttttgc ctgattcctt
    46621 atcgacgtaa ggtttgccgg ttttcgggtc attccccatg aaggcgctgg cgtccaggtt
    46681 cttaccgata taggtcgcga tatagcttgc cggtgtgcct tttgccgggt cgattttttc
    46741 gcatttaaaa cgtggggaga tatcatcacc cagctctgcg cggtcctccc tgatggcggt
    46801 gttgcgcatg atttcagtga tacgggcttc gttttccggg ctggtgaata ccagcatgtg
    46861 ccagtgcggt gtaccgtcgt ggtggggctc tactgtgcgc acgccgtacc agcccagccc
    46921 ggccttgttg atggccttgc ggacggcggc gaaaaagcgg taaaccagat aatcactggc
    46981 atcacgtacc gttgctccgc agtatttcgg gttcagactg ccgtcatcgt tcacgctgtg
    47041 aaaacgggaa ggggctgtga cggtgacgaa aaacgccacg tcgccgcgtg cttcggcgac
    47101 ctgctccatt ccccggacgc aggccatcat ctcgttacgg cggttacgcg gattagagag
    47161 acctgcccag tagatatcct caaggctgaa ccgttctccc tcttcgttct cgatgtccca
    47221 ggccttcaga aaatcgcggg tgcgctggcg tttttcgcgc cagtccagta acgcttcatg
    47281 gctgatatat accgatgtct tacgtgacac cagacctgcg gcgcgtaaca gctcttcacg
    47341 ccagatgtcc cggaggtgtc ttaactgact gttccaccat gtcgcacagg aaaggcgcat
    47401 gaccgcaccc ggcacctgtt ccgggtcggg ggcggtgcgg cggtcgggcc tgatttccag
    47461 cgacggccag cagggtggcg tgatacccag ttttaacgcc atgtgtgcca gtggctggta
    47521 ggtcagtaac caggtgaaca ggtcatcgtc acccctgccg gtcttttcga tgacctcatc
    47581 acatgcgcgc tccagtgcgg tggaaaacgc ggtggcggtc agggtggcaa ggcgctggat
    47641 ttcgtcacgg ctcagctgcg gcagcataag cagttcatcc agccgttcgc gcgcggcggt
    47701 ggccttaaag gccggggtgg caaagcgatc atttacacgg gcgatacgtt tcaggcgggc
    47761 acgcagcccg tgacgaacga agttatccgc cgcgtatgcg ctgttgttct taagcccttc
    47821 ccggcgtgcg ctctcctgtt tgcggctcag gcgtgccagg tcacggatga gcggaccggc
    47881 aagaaacggc ggcagggcgc gcagctctgc catggcttca gataccagcg ttggcggatt
    47941 ctcgtgtttg tgccgcgcca gtttttccag tctgatccga cgctcttcgt cgtggtgaag
    48001 atgcgaatta attcgcttgc cttcgtctgt gtttgtcagg aagtcgacgg tctcttcatc
    48061 cagtcttgca tcaatgcgaa tgtcttcgtt gatgtagctc tgaatggcct ggcgcaatgc
    48121 ggttggttgc gctctgccga atgtttcagg atcgataacc ggtagcgggg cgttccacgg
    48181 gtacgccagt tgtgcgttat ggtcgctcat gcgtcgcact ccccggcagt cgccgcctga
    48241 aacgccgcgc ggtacagcac gagaaggcgc tggtattcac cgataaagtc ttctgcggtg
    48301 tatatgcctt tcacggccac gctgtggttt gtcatttcca gaacaagcgt catctctgac
    48361 gacgaccagc tgatggcggt gctgtcggtt ccgttgatgt gaaataaacc aaaaccatca
    48421 cctttcacgt ccacgcggta acggtcgtca acggtgaagg agaaatcccc gtatgtggtg
    48481 acgcgtctgg cggcctgttt acaggcgcgg cggtaatagt cgtgccagtc gttgaggcgg
    48541 gaagccagcc ggggctccac tgcccacatc cagttaaaaa aatcctgaat atctgaaagt
    48601 cccttaacgt tcttcatg
//
LOCUS       DAZFRN010000018        44890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFRN010000018 DAZFRN010000000
VERSION     DAZFRN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.018"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(345..887)
                     /gene="ycfP"
                     /locus_tag="ACHKUB_000997"
     CDS             complement(345..887)
                     /gene="ycfP"
                     /locus_tag="ACHKUB_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO6654046.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQS
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(910..1935)
                     /gene="nagZ"
                     /locus_tag="ACHKUB_000998"
     CDS             complement(910..1935)
                     /gene="nagZ"
                     /locus_tag="ACHKUB_000998"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO6654047.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1946..2770)
                     /gene="thiK"
                     /locus_tag="ACHKUB_000999"
     CDS             complement(1946..2770)
                     /gene="thiK"
                     /locus_tag="ACHKUB_000999"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO6654048.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2751..3392)
                     /gene="lpoB"
                     /locus_tag="ACHKUB_001000"
     CDS             complement(2751..3392)
                     /gene="lpoB"
                     /locus_tag="ACHKUB_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO6654049.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLALSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3406..3783)
                     /locus_tag="ACHKUB_001001"
     CDS             complement(3406..3783)
                     /locus_tag="ACHKUB_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO6654050.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3786..4145)
                     /gene="hinT"
                     /locus_tag="ACHKUB_001002"
     CDS             complement(3786..4145)
                     /gene="hinT"
                     /locus_tag="ACHKUB_001002"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO6654051.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4479..6668
                     /gene="fhuE"
                     /locus_tag="ACHKUB_001003"
     CDS             4479..6668
                     /gene="fhuE"
                     /locus_tag="ACHKUB_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO6654052.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMSLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(6728..8161)
                     /gene="ptsG"
                     /locus_tag="ACHKUB_001004"
     CDS             complement(6728..8161)
                     /gene="ptsG"
                     /locus_tag="ACHKUB_001004"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO6654053.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8456..9253)
                     /locus_tag="ACHKUB_001005"
     CDS             complement(8456..9253)
                     /locus_tag="ACHKUB_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6654054.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVNFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9264..10268)
                     /gene="holB"
                     /locus_tag="ACHKUB_001006"
     CDS             complement(9264..10268)
                     /gene="holB"
                     /locus_tag="ACHKUB_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO6654055.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10265..10906)
                     /gene="tmk"
                     /locus_tag="ACHKUB_001007"
     CDS             complement(10265..10906)
                     /gene="tmk"
                     /locus_tag="ACHKUB_001007"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6654056.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10896..11918)
                     /gene="yceG"
                     /locus_tag="ACHKUB_001008"
     CDS             complement(10896..11918)
                     /gene="yceG"
                     /locus_tag="ACHKUB_001008"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO6654057.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11921..12730)
                     /gene="pabC"
                     /locus_tag="ACHKUB_001009"
     CDS             complement(11921..12730)
                     /gene="pabC"
                     /locus_tag="ACHKUB_001009"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO6654058.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12850..14091)
                     /gene="fabF"
                     /locus_tag="ACHKUB_001010"
     CDS             complement(12850..14091)
                     /gene="fabF"
                     /locus_tag="ACHKUB_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO6654059.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14179..14415)
                     /gene="acpP"
                     /locus_tag="ACHKUB_001011"
     CDS             complement(14179..14415)
                     /gene="acpP"
                     /locus_tag="ACHKUB_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6654060.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14626..15360)
                     /gene="fabG"
                     /locus_tag="ACHKUB_001012"
     CDS             complement(14626..15360)
                     /gene="fabG"
                     /locus_tag="ACHKUB_001012"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO6654061.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15373..16302)
                     /gene="fabD"
                     /locus_tag="ACHKUB_001013"
     CDS             complement(15373..16302)
                     /gene="fabD"
                     /locus_tag="ACHKUB_001013"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO6654062.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16318..17271)
                     /gene="fabH"
                     /locus_tag="ACHKUB_001014"
     CDS             complement(16318..17271)
                     /gene="fabH"
                     /locus_tag="ACHKUB_001014"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO6654063.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17339..18409)
                     /gene="plsX"
                     /locus_tag="ACHKUB_001015"
     CDS             complement(17339..18409)
                     /gene="plsX"
                     /locus_tag="ACHKUB_001015"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO6654064.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18490..18663)
                     /gene="rpmF"
                     /locus_tag="ACHKUB_001016"
     CDS             complement(18490..18663)
                     /gene="rpmF"
                     /locus_tag="ACHKUB_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO6654065.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18715..19236)
                     /gene="yceD"
                     /locus_tag="ACHKUB_001017"
     CDS             complement(18715..19236)
                     /gene="yceD"
                     /locus_tag="ACHKUB_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO6654066.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19435..20019
                     /gene="yceF"
                     /locus_tag="ACHKUB_001018"
     CDS             19435..20019
                     /gene="yceF"
                     /locus_tag="ACHKUB_001018"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO6654067.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20131..21090)
                     /gene="rluC"
                     /locus_tag="ACHKUB_001019"
     CDS             complement(20131..21090)
                     /gene="rluC"
                     /locus_tag="ACHKUB_001019"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO6654068.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21663..24848
                     /gene="rne"
                     /locus_tag="ACHKUB_001020"
     CDS             21663..24848
                     /gene="rne"
                     /locus_tag="ACHKUB_001020"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO6654069.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSCGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQETPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25045..25998)
                     /gene="flgL"
                     /locus_tag="ACHKUB_001021"
     CDS             complement(25045..25998)
                     /gene="flgL"
                     /locus_tag="ACHKUB_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO6654070.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26010..27653)
                     /gene="flgK"
                     /locus_tag="ACHKUB_001022"
     CDS             complement(26010..27653)
                     /gene="flgK"
                     /locus_tag="ACHKUB_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO6654071.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFCAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27719..28660)
                     /gene="flgJ"
                     /locus_tag="ACHKUB_001023"
     CDS             complement(27719..28660)
                     /gene="flgJ"
                     /locus_tag="ACHKUB_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO6654072.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28660..29757)
                     /gene="flgI"
                     /locus_tag="ACHKUB_001024"
     CDS             complement(28660..29757)
                     /gene="flgI"
                     /locus_tag="ACHKUB_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO6654073.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29769..30467)
                     /gene="flgH"
                     /locus_tag="ACHKUB_001025"
     CDS             complement(29769..30467)
                     /gene="flgH"
                     /locus_tag="ACHKUB_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO6654074.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30520..31302)
                     /gene="flgG"
                     /locus_tag="ACHKUB_001026"
     CDS             complement(30520..31302)
                     /gene="flgG"
                     /locus_tag="ACHKUB_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO6654075.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31440..32195)
                     /gene="flgF"
                     /locus_tag="ACHKUB_001027"
     CDS             complement(31440..32195)
                     /gene="flgF"
                     /locus_tag="ACHKUB_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO6654076.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32215..33420)
                     /gene="flgE"
                     /locus_tag="ACHKUB_001028"
     CDS             complement(32215..33420)
                     /gene="flgE"
                     /locus_tag="ACHKUB_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO6654077.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMKGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33445..34140)
                     /gene="flgD"
                     /locus_tag="ACHKUB_001029"
     CDS             complement(33445..34140)
                     /gene="flgD"
                     /locus_tag="ACHKUB_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO6654078.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34152..34556)
                     /gene="flgC"
                     /locus_tag="ACHKUB_001030"
     CDS             complement(34152..34556)
                     /gene="flgC"
                     /locus_tag="ACHKUB_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO6654079.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34560..34976)
                     /gene="flgB"
                     /locus_tag="ACHKUB_001031"
     CDS             complement(34560..34976)
                     /gene="flgB"
                     /locus_tag="ACHKUB_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO6654080.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35131..35790
                     /gene="flgA"
                     /locus_tag="ACHKUB_001032"
     CDS             35131..35790
                     /gene="flgA"
                     /locus_tag="ACHKUB_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO6654081.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35866..36159
                     /gene="flgM"
                     /locus_tag="ACHKUB_001033"
     CDS             35866..36159
                     /gene="flgM"
                     /locus_tag="ACHKUB_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO6654082.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36164..36580
                     /gene="flgN"
                     /locus_tag="ACHKUB_001034"
     CDS             36164..36580
                     /gene="flgN"
                     /locus_tag="ACHKUB_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO6654083.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMVQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36620..38155)
                     /gene="murJ"
                     /locus_tag="ACHKUB_001035"
     CDS             complement(36620..38155)
                     /gene="murJ"
                     /locus_tag="ACHKUB_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO6654084.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38265..39188)
                     /locus_tag="ACHKUB_001036"
     CDS             complement(38265..39188)
                     /locus_tag="ACHKUB_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6654085.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39190..39837)
                     /locus_tag="ACHKUB_001037"
     CDS             complement(39190..39837)
                     /locus_tag="ACHKUB_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO6654086.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39848..40432)
                     /gene="rimJ"
                     /locus_tag="ACHKUB_001038"
     CDS             complement(39848..40432)
                     /gene="rimJ"
                     /locus_tag="ACHKUB_001038"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6654087.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40668..41876
                     /gene="mdtH"
                     /locus_tag="ACHKUB_001039"
     CDS             40668..41876
                     /gene="mdtH"
                     /locus_tag="ACHKUB_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO6654088.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41940..42587
                     /gene="grxB"
                     /locus_tag="ACHKUB_001040"
     CDS             41940..42587
                     /gene="grxB"
                     /locus_tag="ACHKUB_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO6654089.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42721..43281
                     /locus_tag="ACHKUB_001041"
     CDS             42721..43281
                     /locus_tag="ACHKUB_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6654090.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43387..44433
                     /gene="pyrC"
                     /locus_tag="ACHKUB_001042"
     CDS             43387..44433
                     /gene="pyrC"
                     /locus_tag="ACHKUB_001042"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO6654091.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEATVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44507..44752
                     /gene="dinI"
                     /locus_tag="ACHKUB_001043"
     CDS             44507..44752
                     /gene="dinI"
                     /locus_tag="ACHKUB_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO6654092.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10849 a        12133 c        11173 g        10735 t
ORIGIN      
        1 acaacaggtt acagcaatga aaatgttctt cgctattgag aataacatga atggtgcatt
       61 ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttactg
      121 ggcgagatca aaaaatagag tttattgttt attatttgag catattcgga agagtaatat
      181 agggcgaagc gtatatcgag ggcgtttggt cgtcctgata agatgcgcag catcgcatca
      241 ggcattgatg ctcgactgtc ggatgcggcg taaacgcctt atccgaccca cagaatccgg
      301 agtgcatcag gtaccggcaa ggttacctga tgcgacgagg gcatttaccc gagggttttg
      361 aacgctttaa tgcgctgcaa atgcggggag atattcttga atttgtgcga ctgctcttcg
      421 tcccagacaa tctcgtaata atgatgcagc tcttcagatg tccgctggct gttaagcgct
      481 tcatcattac gcgacaaaat caccaggcaa cgatcgcgat tcttctcacg gaagttggtc
      541 acacacttag tggcaatatc ggcatactct tccgggcgat caatcttgcc ttccatgttc
      601 tcataaggga acaaattagg gttgaagatc acctggcgga tgtcgcaaag aaaaccaatc
      661 cgttccgccc agtatccgcc caggccaacg ccgcaaatta gcggacgctc gtcaacgttc
      721 agttgcaaca ttttgtccac ttctttgagc agatgctgca tatcatgttt cggatgccgc
      781 gtactgtagc ttatcaagcg tacatccggg tcaataaact gcaattgtaa gactttctcg
      841 tggttacccg gactgttaga gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat
      901 aagccagggt tagtgacctg ctttctcttc ctgccagcgt tcgtgtaact gattcagacg
      961 ggtgctgatc gctttccagc gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt
     1021 atgatacaaa cgtgtaacac gttctgcctt gatcggtgac agattatcta acacgctgac
     1081 ggccccttta cgattattgc agaccaggat catatcgcaa cccgcatcca gtgatgcctg
     1141 cccgcgttcg gcataactgc ccataatcgc ggcaccttcc atcgataaat cgtcagagaa
     1201 aatcacgccg tcaaaaccca gttcctgacg caaaacggtt ttcagccagt agggagaacc
     1261 gctcgccgga cgcggatcaa catcactgta gatcacatgc gcaggcataa tggcgtcgag
     1321 tttattttcg cggattaagg aactgaagac cgacatatct ttagcgcgaa tctccgcttg
     1381 tggacgtgga tcgcacggtg tttctttgtg tgagtctgcc gttactgcac cgtgtcctgg
     1441 gaagtgtttc ccggtcgttt tcattccggc ttcatgcata ccatcaataa accggctggc
     1501 aattgccagg gctttttgtg gatcggcatg ataagaacgc tcgccaatcg ccgcgctgat
     1561 atgcccgaca tccagcactg gcgcaaagct gatatcaata tccatagcga tcatttcgct
     1621 ggccatcagc caacccgcct cttgcgccag tttgccgccc tcttccattc ctgacagcgc
     1681 agcgaatgat tgcgccgctg gcaagcgggt aaaaccttca cgaaaacgct gcacgcgtcc
     1741 accttcctga tctaccgcca ccaccagatg attgcgcgaa gctgcgcgga tctggcgcac
     1801 cagttcacgt aactgggcag gatcatgata gttacgcgta aagagaatca gccctcccac
     1861 cagcggatgc gcaagtattt cacgctcttc cgcgtccagt tcgtaacctt tgacatccaa
     1921 cattactgga cccacactgc tctccttatt gttttattaa tagctgccgc caggtgtcat
     1981 cggccagcct gataaattgt tgatcgccgg tttgtcgcca gcggtactca aaccaccctg
     2041 ctttgagcat cagcagccag ggaaaccatc gcctgacctg acgccataat tgcgccggat
     2101 aaatcttcgc gcgagtggca tagtcattga ccaattgccg gtgctgttca gtattttcca
     2161 cccacaccgc cgccagttcc agcgcgatat caccatctcc ggcatactcc cagtcgatga
     2221 gttttaaccc tgacgcgcta tgcactaaat ttccggcgtg gacatccata tgcaatggac
     2281 ttaagcgtaa aggccgtggt tcccgcgctt tgcgcagacg ttttaacatt cgcagccaac
     2341 ccactgtccg ccgcgccgga tcgctttgct gccagtacag ttccagtaac ggcaacagcg
     2401 ttattcgcca gccaaaacgt ggttgttgat gtagataata cagcaagcct gccagttcgt
     2461 tggtatccgg caaatacgtt tttaccgcgc cgggcagata gtcgactacc atccagtcac
     2521 ggagatataa atgcggcttc ggtgcaatgc ttgcgggtag ttgtgataaa gcccgatact
     2581 ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg ctgacgcaca acaaaacgct
     2641 ggccctgatg ttcaatgaga aaactcccgc cactaagccc actattaaac gtcgtgacgg
     2701 gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg catgatggga ttattgctgc
     2761 gaaacggcac ctttacctga ccagataatt tcgcccgtct gcaccagcat cagctgcatt
     2821 tgtagggtcg gagcgttaac gttgccagag gcgctggagt acagcacgta atgagcgccg
     2881 acattgcggg caatgcctat ggctttacta cgggtaccta aactgtcctg cggcgacaaa
     2941 gctaactgtt gcttagccat cgacagctgc tgggcggaaa ccagggtaaa tttcccgtta
     3001 ttagccagcg catttcgcag cgtttcggtc gcttctgcgg cattcagcga accgttagta
     3061 cggttgttaa cgctatcaac cagcaggacg ctacccgcag tcaccccgtc agccccaagc
     3121 atcttactga ccatcggctg cattgcgcca ttccagtcat aatggcgaat atgcggcgca
     3181 ggcggtgccg tttgatcttc gtgctcaatt gggcctggct gctgcgggat cgtcggcacc
     3241 gagggcactg tggggacagg ctgttgtggc tcggctggtt gttccggcgc tggtttcact
     3301 tcttctaccg gtgcaggttc acgttgcccc acacacccgg cgagaaacat cgccagcgcg
     3361 gtaatcaagg cgtagcgact catttttgtc atcaagattc accccttaca aatatagata
     3421 aagtctgact ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg
     3481 tgcgggaatg ctaacgctgc gtggcctttc cagaggatgc atctccagcc ctctggcgtc
     3541 ataccagtaa aaacgataat gaacggtaac gggttcttgc ctttcgttat aaagcgttga
     3601 ggatgctgaa ggttgaatat cagacgtcga aaggacgggc ttttctgcac tgatgcccgc
     3661 agccaataaa gatgactcca tcaccagcga ttgctcatca ttcaccggaa tttccggatg
     3721 tgaacgacag cccaccagca gtaacaacgc cagagacacc agcccaaagc atccttttct
     3781 catcgttaca gacctttatg cgccagcatt ggtcccagcg gacggccacc caacaagtgc
     3841 atatggatgt ggtaaacctc ttgtccgcca tggcggttgg tattcatgat cagacgatag
     3901 ccatcttcgg caataccttc ttgctcagca atttttgccg ctacggtgat catgcgtccc
     3961 agcgcctgct catgctcagc tgacacgtcg ttcacagtcg gaatgaggat attcggaatg
     4021 atcagaatat gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc
     4081 tggtagacga tatcggaggg gatctcacga cgaataattt tgctgaatat agtttcttct
     4141 gccacgacat tttccttttt caaatagcca tgcgttgcgc catgctacgc cgcgccacac
     4201 tgcgagtata gagtatggtc gagttaaacc tgctctttca accttaactc acgatttctt
     4261 aagcaaaaaa tcgttttgct gccgtatata tcgccattat tcccatttct gcctgcgaat
     4321 gcgctgaaaa gacattctct gtttggttta cacgacaaat atgaatgcgt atatttctca
     4381 tttgcattta caaacaaaat tattcgcaca ctaaatataa ccgctcatca ccctcctccg
     4441 gcatgagcct gacgacaaca taaaccaaga gatttcaaat gctttcaaca caatttaaca
     4501 gggataatca atatcaagcc agcaccaaac catcactact tgccggttgc atagcattgg
     4561 cactattacc ttctgccgct tttgccgctc cagccactga agaaacggtg attgttgagg
     4621 gttcagccac agctccagat gatggcgaaa atgattacag cgtaacgtct acctctgcgg
     4681 gtaccaaaat gcagatgact caacgtgata ttcctcagtc ggtcactatt gttagccagc
     4741 agcggatgga agatcagcag ttacaaacgc tgggcgaagt gatggaaaac acgctgggaa
     4801 tcagcaaaag tcaggcggat tccgatcgtg ctctttatta ttcccgcgga ttccagatcg
     4861 ataactatat ggttgatggt atccccacct attttgaatc gcgctggaat ctgggcgacg
     4921 cactttctga tatggcactc tttgaacgcg tagaagtagt gcgtggcgcg acaggactca
     4981 tgaccgggac gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac gcgaccagtc
     5041 gtgaatttaa aggcgatgtc tcggcggaat acggtagctg gaacaaagaa cggtatgtgg
     5101 cggatttaca aagcccactc accgaagacg gtaaaatccg cgcgcgaatt gtcggcggct
     5161 accagaataa cgactcatgg ctggaccgct acaacagtga aaagaccttc ttctcgggca
     5221 ttgtcgatgc tgatttaggc gatcttacga cgctttcagc cggttacgaa tatcagcgca
     5281 ttgatgttaa tagccctacc tggggcggtt taccgcgctg gaatactgat ggcagcagca
     5341 acagttacga tcgtgcacgc agtaccgcac ctgactgggc gtacaacgat aaagagatca
     5401 acaaggtctt tatgtccctg aagcagcggt ttgctgatac ctggcaagcg acactgaatg
     5461 ccacccactc tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc tatgtaaaca
     5521 aagcggatgg tatgctggtt gggccataca gtaattatgg acctggcttt gattatgtcg
     5581 gcggcaccgg ttggaacagt ggcaaacgta aagttgatgc gctggatttg ttcgctgacg
     5641 gtatttatga attgtttggt cgtcagcaca atctaatgtt tggtggcagt tacagcaaac
     5701 aaaacaatcg ttacttcagt tcatgggcca acatcttccc ggatgaaatt ggcagtttct
     5761 acaactttaa tggcaatttc ccacaaaccg actggtcacc acagagcctg gcgcaggacg
     5821 ataccacaca tatgaaatcg ttatatgctg ccactcgagt cacccttgcc gatccgctgc
     5881 atctgatcct cggcgcacgt tataccaact ggcgggttga tacgctgact tacagcatgg
     5941 agaaaaacca caccacgcct tacgctggtc tggtgtttga catcaatgac aactggtcga
     6001 cctacgccag ctatacctct attttccagc cgcaaaatga tcgtgacagt tcaggcaaat
     6061 atctggctcc aatcaccggt aacaactacg agctgggtct gaaatcggac tggatgaata
     6121 gccgtctgac caccacgtta gccatcttcc gtattgagca ggataatgtc gctcagtcca
     6181 ccggtacacc tatccccggc agcaacggcg aaaccgccta taaagcggtg gatgggacag
     6241 tcagtaaagg ggtggaattt gaactcaacg gcgcaattac cgacaactgg cagctgacat
     6301 ttggcgcaac gcgctatatt gcagaggata acgaaggaaa cgccgttaat cctaatctgc
     6361 cacgcaccac ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca gagttgacag
     6421 tcggcggtgg tgttaactgg caaaatcgcg tgtataccga caccgtgaca ccgtatggca
     6481 ccttccgcgc cgagcaaggt agctacgcgc tggtggatct cttcacccgc taccaggtga
     6541 cgaaaaactt ctcgttacag gggaacgtca ataacctgtt cgacaaaacc tacgatacca
     6601 acgtggaagg ttctatcgtc tacggcacac cgcgtaattt cagcattacc ggcacgtatc
     6661 aattctgaat aacacctgta aaaaaggcag ccatctggct gccttagtct ccccaacgtc
     6721 ttacggatta gtggttacgg atgtactcat ccatctcggt tttcaggtta tcggatttag
     6781 taccgaaaat cgcctgaaca ccagaaccag caaccactac gcccgctgcg cccagtttct
     6841 tcaggccggc ctgatccact ttagacacat cagcaacgct gacgcgcaga cgggtaatac
     6901 atgcgtcgag gttagtaatg ttttctttac caccaaatgc agcaaccaga gccggtgcca
     6961 tttcgctggt acctgtcgct tttgcatctt cagtcgcgtc ttcacgaccc ggcgttttca
     7021 gatccagtgc tttaatcagc acgcggaaga tggtgtagta aacaatcgca taaccgatac
     7081 cgacgatcgg gaacagccac agtttgctgc tgttaccaga cagaacgatg aagtcgatca
     7141 gaccgtgcga gaacgacgta ccgtcacgca tccccagaag aatacagatt gggaatgcca
     7201 ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg aaggagaact
     7261 cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc ataataccgc
     7321 ccactttcgc gcggttttct ggtttagcag agtgccagat agcaattgcg gcagctggca
     7381 gaccgtacat tttgaacagg aagccaccag acagtttacc cgcagtcggg tcacccgcca
     7441 tataacgcgg aatgtcgccg tggaaaacct gacctgctgc gttggtgtat tcaccaatct
     7501 gcatctggaa aggtacgttc cagatgtggt gcagaccaaa cggtaccagg caacgttcga
     7561 tgaaaccgta aatgccaaac gcaacaaccg ggttctggta agcagcccac tgagagaagg
     7621 tctggattgc agaaccaatc ggcggccaaa tgaaggacag cacaacgcca gtaaagatgg
     7681 cagccaggcc agaaatgatc ggcacaaagc gtttaccggc aaagaagcca agatactcag
     7741 gcagcttaat acggtagaaa cggttaaaca tgtacgctgc gatcgcaccg gagataatcc
     7801 ctccgagtac gccagtatcc gccaggtgtt tagaggcgat ttcttcagca ggtaaatgca
     7861 gtaccagtgg cgcaaccacg gccatggttt taaccatgat gccataggca acaactgcgg
     7921 ccagcgcgga tacgccatcg ttattggtaa agccgagggc gacaccgatc gcaaaaatca
     7981 gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat aacatgcgat acaacggcgg
     8041 gcagccagct gaaattcgcg gaaccgacgc ccagcagaat acctgcgata ggcagtacgg
     8101 ataccggcag catcagcgat ttaccgacct tttgcaggtt agcaaatgca ttcttaaaca
     8161 taattgagag tgctcctgag tatgggtgct ttttttacgt tctcacgcgt ggcaaggggg
     8221 gagagcctcg ccgtgtacag ggcatctaag cgccctttat ttattacaca gagtaaaata
     8281 attcagtgcc aatttgtttg acggctatca cgtttcaact tcagatcatt ggagaagttg
     8341 cacattttga caaaacatat gtcacaatat ttcaaacgtg gttattcacc gcccaatttt
     8401 cggcggtgaa ctttactcgt tttagccatt aattacgagc tttaaaaaaa ctcattcaac
     8461 ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta tcggtggtta
     8521 cctgcgccag ttcttcaacg gcaacacctt tcaacacagc catgtattct gcaacgtcac
     8581 gaaccatcgc aggttgattc tcttttcctc gatgcggtac cggcgcaagg taaggtgagt
     8641 cagtttccac cagtaatcga tccaggggga cataacgcgc agcatcgcgc agttgctccg
     8701 cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc agtaatttac
     8761 ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc gtcacttttt
     8821 cttcgcgcag aattgccagc gtatcggcac gggcgtcacg ggtatggacg ataaccggct
     8881 tgttcagttc acgaccaatc tggatatgat ggatgaagga ctcttgctga cgtactttag
     8941 tttccggtgt gtaataataa tccagcccgg tttctcccag cgctacaaca ccctcttctg
     9001 ccgccagacg gcgtaaatct tctacatcgt agggatcatt ctggttgagt ggatgcacgc
     9061 cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc ccgcatgtgt aaatagcccg
     9121 gtaatgttgt ggcgactgcc agacaaaaat tcacatcgcg tgcggcggct ttcgccagaa
     9181 cgtcatccac gtccttatgc aaagattcat aatccagacc atcgagatgg cagtgtgagt
     9241 cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg gtagcacaac gcccggttgc
     9301 aggtaatgct caatacgcag taaaagatcg gtgatgagaa gctcgcggtt gatgcctgtg
     9361 acagacatta actgttcacg aatgtggcaa acatccccca gtatagcctg caggcgcgag
     9421 ggagaaagat ggtttgccag ttcggcgacc aggcccggca catcaacatt ggtcacctgc
     9481 gcagcgccat gatggcgttt tagcgcatcc atcagcaacg ttgccagcca gtgtaaacgc
     9541 gccggagctt gttcatgatt aagggccgcc agcagcgaat accagtcgcc cgatggtacg
     9601 ctatatgcca acgcctgaca caatgtttca cgagcctgcc agttatctcc ctgaaacaac
     9661 gccagtgccg cgccaggcga accggcgctt aagcgcaatg cggcaagtaa tgcatcctgt
     9721 gacattgtca cttcgcgtga aagccaggtc acggcgtact gttccggcgg cggcgcaagg
     9781 taatgtaacc gacaacgact acgtaatgtt gccagtaaac gttcaggctc gcgggtagcc
     9841 aggaaaaacc aggtttctgc tggtggctct tcaagcgttt tcagcaatgc gttagccgcg
     9901 gcgtcggtta gtaaggcagc atcggttacc cagacaactt tcgcaccacc taagcgtgcg
     9961 tgctcattca gcttttcggt gacctcacgt accgcatcaa tgcccaacgc atttttccct
    10021 ttttcgggag ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa ctgacatcca
    10081 cgacagtgac cgcaactttt gtgaccctgc ggttgttggc agagtaaata acggctcagg
    10141 gcgtagatta aagcatcatc gcccatgccc ggtaacgcct gaatgagcag cgcatggtga
    10201 cctcttccgg cctgatagct ggctaccagt ttttcgaaat caggtcgtaa ccatggatac
    10261 catctcatgc gtccaactcc ttcacccagt gggtcacggt agtgcggatt gcatccatca
    10321 cggcctccag cggctgggtg gcatcaatgg tatgaatgct tttatcttgt gctgccagtt
    10381 ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca atacgatcca
    10441 gctcgccgcg cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg agatagagcg
    10501 ttaagtcggg gcgaaaatcc ccgagaacag catcacgcag tgttgccagc atatgttggt
    10561 caataccacg tcctccgccc tgatacgcct gagtggagag atcgtggcga tcgccaatca
    10621 cccaggtgcc gttagccagc gcaggtttga tgactgtttc taccagttga acgcgcgcgg
    10681 cataaaacat cagaacttcg gctttatcgg taatgacttc atcgcctacc gatttgatat
    10741 ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc cgagtgaaaa
    10801 ccatgtcgcg gatacccagt tgctcgagcg tctcaaccac cacattacgc gcggtagttt
    10861 tgcctgcgcc ttccagcccc tcaatgacga tatacttact gcgcattttt ttccttaagc
    10921 actttcagat aatcctgcac agacttgtta tgactggcaa gattggtatt aaacgtgtga
    10981 ccacctttac catcggccac aaaatagaga tacggcgttt ttgccggatg cgcagcagcc
    11041 ttcagcgaat ccgcccccgg cgtagctatc gcacccggcg gcaaaccggt aatggtatag
    11101 gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt ataacgctct
    11161 cccatcccgt aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg taaacggttg
    11221 ataaataccg aggcaacctg atcgcgttca ctggcaacgg cggtttcttt ttcgataatt
    11281 gatgccatcg tcaccagctg atttttatct ttataaggca gaccgtccgc acgcccttcc
    11341 caggcgctat cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa cgcgacatcg
    11401 gtggtattgg cggtatacat ccaggtgtct ggccagaacc aaccttcaat ccactccggg
    11461 ttttccagtt caagtgcctg cgctacggtg gcgtacttat cgtcactcag cgtgtgcttg
    11521 atatacggag cctcacgcaa ttgcttgagg tagtcgctca gacgcatccc ttctaccagt
    11581 cgcagcggga actgtgcttc tttaccgctt tccaacaatt tcagcatctc gcgcacggtc
    11641 atctgcggtg taaagcggta agtcccggct ttaaagtgag aaagatccgg ttcgatacgc
    11701 agcagccatt gaaaaacccg tgggcgattg atgatcttat cagcataaag ctgttcaccg
    11761 agcgccagac gtccggtccc tggcttcagg gtaaatatcg tctcttcttt gataagcaat
    11821 ttgctgtcgg caagatggcg aaccttccag acgcccacac cagcggcgat acccagtacc
    11881 accagcaata acaagataat caataacact tttttcatga ctaattcggg cgctcacaaa
    11941 gtggggctaa atattcatat aacgttgctg acgaaaagga gacatcgcca caggcacgta
    12001 cgggcatcac tggcattaac gcattacaaa taaccatctc atctgcctgc aacgcctctt
    12061 ccagagaggc ttgcacttcg acaagctgat aagaggattg tgccagcaaa cggatacaga
    12121 attgtcgcat aatgccgtta acacctgcct gatccagtcg cggcgtataa actacattgc
    12181 ccttccgcca gaacaaatta gccgcacagc attccgtaac ccacccttcg ctgtcaagga
    12241 ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg gatcaatact tgctcaagcc
    12301 gattaagatg tttaatacct gcaagatgag gattgcgccc cagccgcacc gggcttagcg
    12361 ccaacgtcat cccttcgtta cgcaaacggt cgtaatgtgc aggataaacc gtaacggaga
    12421 gaatccgcgt tgccggtcca ctgttcaatg tgctgtaccc tcgcccgcca ctaccgcgac
    12481 tgatcacgac tttcagcaca ccattttgct gttctgctgc cagcgttttc atctcttgtt
    12541 caagctgagg ccagaagtca cagaaaatca tcaaccgctg acaagcatcc tgtagtcgct
    12601 ggatatgcgc cgataacaaa ctgactttac cgtcgataac tctggcggtg gtaaaacaac
    12661 catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta taaccgttaa
    12721 ttaagaacat accggctcct tatcagagaa ttgttagtgt ggcagcatgt tcactacgga
    12781 acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg gaccttttat atgggtggaa
    12841 aatgacaact tagatctttt taaagatcaa agaaccatta gtgccaccga agccgaagga
    12901 gttacacagc gtgtattcca ttccgctgac ctgacgtgct tcatgcggta cgaaatccag
    12961 atcgcaacct tcatccgggt tatccaggtt gatggttggc ggaacagcct gatcgcgcag
    13021 cgccaggatg gagtagatag attctactgc acccgccgca cctaacaggt gaccggtcat
    13081 agatttcgtg gagcttacca acacacggct tgcagcttca ccgaagatgg ttttcaccgc
    13141 ctgcgcttca gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa cgtagccaat
    13201 ctgactcgct tcaatgcctg catcacgcag agcatttgcc attgccagag ctgcgcctgc
    13261 gccattttct ggcggtgacg tcatatgata agcatcgctg ctcataccaa agccgacgag
    13321 ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg tactcttcaa gtaccagcat
    13381 accggcacca tcgcccagta cgaagccatc acgctcttta tcccacgggc ggctcgccgc
    13441 ttgcgggtta tcattgcggg tagataatgc acgtgccgcg ccaaaaccac caacgcccag
    13501 cggcgtactg gctttctctg cgccacctgc aaccatcacg tcagcatcgc catacgcgat
    13561 aatacgcgca gcatgaccaa tgttgtgcac gccggaagta caggcggtcg cgatagagat
    13621 gcttgggcca cgcaggccat acatgatagt cagatgacct gccaccatgt tcacaatcgt
    13681 tgacggaacg aagaatgggc tgatcttacg tggaccaccg ttcatcagag atgtgtggtt
    13741 ttcttcgatc agtccgaggc cgccaatccc ggagccaatt gcggcaccaa tgcgggttgc
    13801 gttctcttcc gttatttcaa ggccagaatc ctgcatggcc tgaacgccag cgacaattcc
    13861 atattgaatg aaggcatcca tcttgcgctg ttctttgcgc gagataatgt cctcacagtt
    13921 aaaatccttt actaagccag caaatttcgt tgcataggcg ctagtatcga aatggtcgat
    13981 taggctgatg ccactctgac cggcaagcag agctttccag gtagactcta cggtattgcc
    14041 gacaggagac aacatgccca gtccggtcac aactacacga cgcttagaca cgtttgtcct
    14101 ccagggaggg aaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga
    14161 ccgcctggag atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac
    14221 ggtggtgatt ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc
    14281 cattaccagc tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt
    14341 gttggtaact tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc
    14401 ttcgatagtg ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt
    14461 agtgtataaa atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac
    14521 gttattttga ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata
    14581 accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat
    14641 cccgccgttc acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa
    14701 tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag
    14761 gatacccgca cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg
    14821 agcaacaacg tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa
    14881 gccgatcaag cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc
    14941 aaccacagaa ccgatagtga taatacgacc atgacgcttt ttcatcatag cgcgcattac
    15001 cgcttttgac agacggaaaa cagatgaaag gttggtttcg ataatatcgt tccactcttc
    15061 atctttcatt cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc
    15121 cacttcacca aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt
    15181 cacattcaac atcagacctt tgccgttagc acctaaataa tcactgatcg cctgagcgcc
    15241 attttcactg gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat
    15301 tgcgcggcca attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat
    15361 gattttcctc ttttaaagct cgagcgccgc tgccatcgct gaaggttcgt tcagcgccga
    15421 ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagtt agcactttgc ccgggccgac
    15481 ttcatagaga tgttctacgc cttgcgctgc catgtactca acagacttcg tccactgaac
    15541 cgggttatac aactgacgta ccagtgcgtc acgaatggca tcaccattgg tttcgcattt
    15601 cacatcaacg ttattcacaa caggaactgt tggtgcgtta aaggtgattt tcgctaattc
    15661 tactgccagt ttgtcggctg ctggtttcat cagcgcacag tgagacggta cgctcactgg
    15721 taacggcagc gcgcgttttg cgcccgcagc tttacaggca gcgccagcac gctcaaccgc
    15781 ttctttatga ccggcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac
    15841 ctgaccttct gcagcttctt cacacgcttt cgcaatagac gcatcatcca gaccgatgat
    15901 tgccgccata gcgccagtgc cttccggtac ggcttcttgc atgaacttac cgcgcatctc
    15961 aaccagacgc accgcatcag cgaaatcaat cacgccagcg caaaccagcg cggagtattc
    16021 ccccaggctg tgaccggcca tcattgccgg tgctttaccg ccctgctgct gccatacgcg
    16081 atacagcgca acagatgcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc
    16141 ttcagctggc ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc
    16201 agcaaacgtt tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt
    16261 ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta
    16321 gaaacgaacc agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa gcagaaccaa
    16381 ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc
    16441 cgcagaggta ttaccgtggc gatccagcgt caccacgaca ttgtccatag acataccgag
    16501 ttttttcgcc gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag
    16561 ttgagaacgg tcaagattat ttgccgccag cgtctcatca acgatgtgcg ccagttccgt
    16621 taccgcaacc ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt
    16681 cacacggtca gcattaggca gcgtcagcaa ctcaccatag ctaccgtcgg catgcagatg
    16741 ggtggagatg attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc
    16801 aaaaataata atagtcccac gatcggttgg atcgcaggtg cgcgccagta catcggaacc
    16861 gacgaccaga gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc
    16921 ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt taatgcccag
    16981 catgctttga atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac
    17041 aacgatcagg ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc
    17101 ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc
    17161 ggtacgagtg acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt
    17221 ccgcacttgt tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca
    17281 gtcacttttc ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct
    17341 accgcagagt tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt
    17401 ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa
    17461 tcgcgaccgc aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca
    17521 atcctaacag acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca
    17581 ggctcttttg tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac
    17641 cctgagattt cagcagagaa aggaacatcc tgacaacacc ttccatcgtc tttaacgtga
    17701 catttcctgt aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg
    17761 cttcaagata gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc
    17821 gaatactgtc gagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat
    17881 tgggaatttc caccacctct tcagccagga ctgagcccat aatggcaaat tgcaccaaca
    17941 ttgtgctgtc acaatcgacg ttggccccta aatcgagaac caccgttttg cccttttgct
    18001 gatgcggcaa taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata
    18061 attttgccag ccccatcagt gccccggtat taccggcact gacacaggct tgcgctcgac
    18121 cttctttcac caactccagg gccacgcgca ttgaactccc acgactggcg cggatagctt
    18181 gcgaaggccg gacatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac
    18241 gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa
    18301 gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag
    18361 ggccaaaatc ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc
    18421 cgcccggttt ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca
    18481 gacgcgtgat tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg
    18541 gtgacgcagg tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag
    18601 cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc
    18661 catggacctt actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt
    18721 acgctttaag ctggctaata cggcaaatgg gtttggcttt tgcgcttctt caggcagttc
    18781 accaaagacc atgtccgctt cggacacttc acagtgttca gaatcatgca ccggaactac
    18841 cggcaaggcg aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc
    18901 gttaacctca atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac
    18961 aggactaaaa caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca
    19021 ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat
    19081 agcgaacgac atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg
    19141 ctcaacctga tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac
    19201 cggatcgaga gtcaggggta attttacctt ttgcataggg cgcgcatatt aactttgtaa
    19261 cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca
    19321 ttgcgggtta ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga
    19381 agcgcatagt ttaaaatggc tatcatcaat acgctatatc tgatggaaaa aaatatgcct
    19441 aaacttattt tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc
    19501 tcttttgaat gtgcagcacc agaggtcgac gaaaccccac gcagcgacga atcaccgcga
    19561 cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg ttatccggat
    19621 catttaatta ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg
    19681 ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc
    19741 tatactggac tggcgttgtt taattcggcg aatgggcatc tgcaaacaga agtggagcct
    19801 tttgacgtcc atttccgtca cctgagcgag gcggagattg ataattacgt gcgtaaagag
    19861 catcccctgc actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag
    19921 cgtttagagg ggcgtgatcc taacacgctg gttggtttac cgctaatcgc gctgtgtcag
    19981 atgttacgtc gggaagggaa aaacccgctg atgggatagt tttttgcgcc ggatgcggtt
    20041 acttcgcatc cggcaatcgg tgctggcggc ctgatgcggc gtaaacgcct tatcaggcca
    20101 accgcccatt atcaggttta tatgcttgta ttagcgcgcg ttacgcagct tttgcaaaca
    20161 acgcttcaaa ccttcatcca tcggagcttc gatacgcatc acctcaccgg tccccggatg
    20221 agtaaacttc aacgctgcag cgtgcaggaa caggcgattt aatcccgtgc ctgcttcagt
    20281 gagctgcctg tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc
    20341 atattgtgta tgcacacgga tctggtgagt acgccctgtt accgggctac aacgcaccag
    20401 ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg
    20461 actcacacgc acgatacgtt cgccgctttg cagaatattt ttcagtaacg gtgcttgaac
    20521 gctcttcaca tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcatccc
    20581 tttttcacgt aattgctcat gcagagaacg caacgccgag cgttttttcg ctaccagcaa
    20641 aacacctgag gtgtcccggt caagacgatg aaccagttca aggaaccgcg cttccgggcg
    20701 caacgcccgc aaaccttcaa taacgccgaa gcttaaacca ctgccgccat gtaccgccgt
    20761 accggaaggt ttattcagca ccaggatgtg atcatcttca tataagatga cgtccgccag
    20821 cgccgccacc ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac
    20881 cggtggaata cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt
    20941 gttcacccgc acttcgcctt tacgcaaaat acggtaaatc atactttttg gtacgccttt
    21001 caattgggta cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac
    21061 aatttttacg gatggagtct ctgttttcat ggttggcgat tctaattagc caacaggatt
    21121 cgcgccactc atttttctat gcttatattt acttttgcac cttattactt cactgcgtga
    21181 tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag
    21241 cgggtgataa atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc
    21301 cgtttccgtg tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga
    21361 ccgatcatcc acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg
    21421 cgggttgcag tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt
    21481 ctttgataat tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt
    21541 tgagctgacg ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac
    21601 ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta
    21661 cgatgaaaag aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag
    21721 atgggcagcg tctgtatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa
    21781 acatctacaa aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt
    21841 acggcgctga acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg
    21901 ctaactacag tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag
    21961 tcattgttca gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta
    22021 tcagtctggc gggtagctat ctggttctga tgccgaacaa cccgcgcgcg ggtggcattt
    22081 ctcgccgtat cgaaggcgac gaccgtaccg aattaaaaga agcactggca agccttgaac
    22141 tgccggaagg catggggctt atcgtgcgca ccgctggcgt cggcaaatct gctgaggcgc
    22201 tgcaatggga tttaagcttc cgtctgaaac actgggaagc catcaaaaaa gccgctgaaa
    22261 gccgcccggc tccgttcctg attcatcagg agagcaacgt aatcgttcgc gcattccgcg
    22321 attacttacg tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg ctcgaactgg
    22381 cacgtcagca tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca
    22441 ccggcgagat cccgctgttc agccactacc agatcgagtc gcagatcgag tccgccttcc
    22501 agcgtgaagt ccgtctgcca tccggtggtt ccattgttat cgacagcacc gaagcgttaa
    22561 cagccatcga catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaactgcgt
    22621 ttaacaccaa cctcgaagcc gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg
    22681 gcggcctgat tgttatcgac ttcattgaca tgacgccagt acgccaccag cgtgcggtag
    22741 aaaaccgtct gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt
    22801 ctcgctttgg cctgctggaa atgtcccgcc agcgcctgag cccatcactg ggtgaatcca
    22861 gtcatcacgt ttgtccgcgt tgttctggta ctggcaccgt gcgtgacaac gaatcgctgt
    22921 cgctctctat tctgcgtttg atcgaagaag aagcgctgaa agagaacacc caggaagttc
    22981 acgccattgt tcctgtgcca atcgcttctt acctgctgaa tgaaaaacgt tctgcggtga
    23041 atgccattga aacgcgtcag gacggtgtgc gctgtgtaat tgtgccaaac gatcagatgg
    23101 aaaccccgca ctaccatgtg ctgcgcgtgc gtaaagggga agaaactcca accttaagct
    23161 acatgctgcc gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg
    23221 aacgtaagcg tcctgaacaa cctgcgctgg caacctttgc catgccggat gtgccgccag
    23281 ctccaacccc agctgaacct gccgcgcctg ttgtagcccc agcacctaaa gctgcaacgg
    23341 caacaccagc atctcctgca caaccagggt tgttgagccg cttcttcggc gcactgaaag
    23401 cgctgttcag ctgtggtgaa gaaaccaaac cggccgagca accagcaccg aaagcagaag
    23461 cgaaaccgga acgtcaacag gatcgtcgca agcctcgtca gaacaaccgc cgtgaccgta
    23521 atgagcgccg cgacacccgt agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc
    23581 gtcgtaatcg tcgccaggca cagcagcaga ctgccgagac gcgtgagagc cgtcagcagg
    23641 ctgaggtaac ggaaaaagcg cgtaccgccg acgagcagca agcgccgcgt cgtgaacgta
    23701 gccgccgccg taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg
    23761 aagagcaatc tgttcaggaa accgaacagg aagaacgcgt acgtccggtt cagccgcgtc
    23821 gtaaacagcg tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg
    23881 tagtcgcacc ggtggttgaa gaaactgtcg ctgccgaacc aattgttcag gaaacgccag
    23941 ctccacgcac agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac
    24001 agcaagaaga gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc
    24061 gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt
    24121 atccaaccca atcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg
    24181 gcaaagtctg gatccgctat ccaattgtac gtccgcaaga tgtacaggtt gaagagcagc
    24241 gcgaacagga agaagtacaa gtgcagccga tggtgactga ggtccctgtc gccgccgctg
    24301 tcgaaccggt tgttagcgcg ccagttgttg aagaagtggc tgaagtcgta gaagcccccg
    24361 ttcagattgc cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg
    24421 ccgcggtaac tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag
    24481 ttgcagaaca cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag
    24541 ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc
    24601 cagtcgtcgc tgaagtcgca gcagaagttg aaacggtagc cgcggttgaa ccagagatca
    24661 ccgttgagca taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc
    24721 cggaggcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg
    24781 ccgcaggtgg tcatacggca acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg
    24841 ttgagtaata attagctcaa agtaatcaag ccctggtaac tgccagggct tttttatttc
    24901 atgtttgaat caacatatta aaattcatca aatggcatcc ttgctaacca acaatgcaaa
    24961 ataggcaaaa aaaatgcccg ccccggcgat cccagagcgg gcagacaaaa catacccagt
    25021 ttcatgatat gttttaaagc gagcttattt gttgagctgg aagagcgaca atccctgcat
    25081 atcggtaaat gctttatacg atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat
    25141 agttgcattc cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg
    25201 atcgctacct aaggaatcca gcgactccag ttcgttcagc tgcgtgccta attccgcgcg
    25261 aacagtcagc acattgttca gcgagttttt cagtccgcgg ttggttttat ctaacgccgc
    25321 agcggcggtt tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc
    25381 actatccagc atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc
    25441 gttgctggta atactgtcga aaattttgtc acccgtgtgc cctatcacca tcgaacgcga
    25501 agcatcgacc tgttgtttaa tactttctgc tccaccgacg tatttccctt tctcttcgct
    25561 aaacggcgca gtctctgttt tataaccggc aaaaatgtag cgcccgttac cgtcagtggt
    25621 gttcgccaga ttcagcaact ggtcacgaag cccctgaata tccgtagcca gcgaggcccg
    25681 gtcatcgtca ctcaaggtgc cattgctggc gtagacaatt ttttcctgag cattctggat
    25741 agcagtggtg acctggctaa gtacactctc ttccagtgac actttttgag tggcgaaagt
    25801 acgtgccagc gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa ccgcttgtga
    25861 tgctgcaatg ggatcgtcag aagggttaac gactcgctta cctgtcgaca tctgctcgcc
    25921 gtacttcatc cattccgcct gagaattggt gatgccacgc atgttttgct ggtacatcat
    25981 ctgggtactg aaacgcatct tatctcccct tagcgaatgt taatcagcgc atcaaaaatc
    26041 gcgtttgccg tctgcagaac ctgcgcattc gccaggtaat actgctgaaa acgttgcaga
    26101 tttccgtact cctcatcgag attgacaccg gaaatcgact gctgctgatt ggaaagctgc
    26161 gtcaccacat taccttgcgt ggcgctactg gttttcaacg tcgcggtttt attaccgata
    26221 tcactcacta acgaggcata agcgtcgtta aaggatttcg caccgcccac cgttttactg
    26281 ttgctttgca gatccagcag ggcctgaccg ttgcggttat cgctatcacc cgcatcttct
    26341 tcgctcgcca tcgctatttt cgcttcgtcg gtgattaata catccatgtt gacgatagcg
    26401 tcacttactg gtttcagcgt gaagctgtcg ttaacagcag gcgttcctgt aaacgtcaac
    26461 tccagaccat caaatgccac tttaccgttg gcatccggcg tcaccgtaaa agtggtatta
    26521 ctggcaaggc gggtgacctg ccactgatta ttatcgaacg agattttgta atcagtcgcc
    26581 agtaccgcgg aggcatcagt taccgtggca ccgatcgcaa cgtcaccttt gtttttcgtg
    26641 ttttgcagaa ccgcgggctt accgatagca cagaaatctt caccggcatc gccgttagca
    26701 tcaaatccgg ctttgtgttg gctgttgaaa gcctcggcaa atgccagcgc cagttgtcca
    26761 agcgtattac gcgtctggtc cagatcctga gaacggaatg tcagaatgcc gcccagcgac
    26821 ccggtattca gtaatttctc cgggatctca atattgcctg ccgtcccatc aacgtaagcg
    26881 acagtcgtac gagaagggtc ggcgctggaa ggaactgccg ccagttgccg cgccgtactt
    26941 ccctgaacca gtgagtaacc attggccatc gtgatgttat aagtaccgcc atcctgaacg
    27001 ctgacttcta cgccaacaat ctggtttaat tcgctcacca gttgatcgcg ttgatccagc
    27061 agattgttag gtgacgcccc tgcccccacg cctgtcaggc gcgagatttg atcgttcagg
    27121 ctggcaattt gtttagcgta attgttgatc tgatcaacgc tggcacctat cgcgatattg
    27181 acctgtttgt cctggtcgcg cagatattga tcggtggttt taaactgatt caccaaccct
    27241 tctgatttcc caatcagcgc ctggcgcgct gccgggtctt ccgcgttact caccagcgtt
    27301 tgcaggctgg tgaagaaatc ctgcatctgt gttgccagcg aagaggtact ggtggagagc
    27361 atattgtcga ttttcgacat ctgctcatag cgggcagtca ggccgctact ttgcgtctgc
    27421 gcagcacgta actggttggt aataaacgca tcatactcac gctgcacacc agaaacgtag
    27481 acgccattgc caacccagcc gccagcgccc aacgtgctat tggcctgcgc cataatagtg
    27541 gtttggcggg tatatccggc aacgttatag ctggagatat tattacttgc cgtatttaac
    27601 gccgcctggg ccgcgttcag tccgctcatg gcgttattaa tcaagctgga catggaggtt
    27661 ccttataagc cttcaattac agagtattat cggcagcgac ccgccggact tgagttattc
    27721 agaacagatt atcaatgttc atactgtagg ttttgctcac cttgtcgctt atcgatttca
    27781 tctgctgaat catgttggtg agtttgcggg catagtgagg atcggtggca tagcccgcgt
    27841 cctgtagggc ctgcgccccc tgttccgcac tcgcggcggt cgtcacggcg gcgtagcgcg
    27901 ggttacgcgt taacagccca acgtaatccg acaaggcttc cagatacgag ctgtagacgc
    27961 gaaacttcgc ttttactttc ttcgcttcgc cgttttcata ttcagtcgtg gtgatttcag
    28021 taactggccc tttccagttg ccagaggctt tgacaccaaa caggttatag ctcggctcgc
    28081 cgttttcgcg gcggatttgc cgttgccccc aaccagattc cagtgccgcc tgagcgagga
    28141 tcaaatgatg tggcacaccg ctttgctggc ttgccagttg ggcgggcagc gagagttgcg
    28201 cgaggaatgc tttactgtca cccggcagcg aatcatcgta gttacgtggc acggcctttt
    28261 gcaccagctg cgaaagcgcc tgattttgat aacgcaccac agtttcgagc gggaatttca
    28321 tcggtgctgc tggcgtggac tcctctggca atggttgttc tggcgtcatc tgtttaacca
    28381 tcatctctgc aagccccaga cctttgcccg ccgtcatctg ttgggcaatt tgctggtcat
    28441 acatactggt atacagacga gtgtgctcgc tgctgaacag gccatctttt ggtaaagcgt
    28501 cgcgcatgct tttcaacatc atctgcacga acatcccttc cacctggcgg gccaccggac
    28561 ggatatttgc cgccggatct tcgcccgctt tcgcctttag ttcgttgagt gattgcgcat
    28621 cccaggccgc acttgccagt agtttgctgt cgctgatcat cagatgattt ccagttttgc
    28681 ccgcagacat cccgcacttt gcattgattg caaaatagac atcagatcca tcggcgtagc
    28741 gcccagcgca ttgagcgcgc gcaccacgtt attgaggctg gcgctggaac gtacgctttg
    28801 cagcgaaccg ccgctctggc gtaaatcgat ctgcgtttgt ggagtaacca cagtctgtcc
    28861 accaccaaac ggtgtatctg gctggctgac attggcctga cgattgactg ttactgagag
    28921 atttccctgc gctaccgcgc agctgtcgag ggtcacttcg cgattcatca ccaccgaacc
    28981 ggtgcgcgag ttaatcacta ctttagcgtc ctgcggggtg acattaacct gcatattctg
    29041 gatatctgca aggaaacgga cctgggaact gttgccactt ggtacgcgca cctgaatagt
    29101 ccgcgcatct aacgcggtgg cgctgccata tccacgcacg cggttgatgg tgtcagcgat
    29161 ttgctgcgcc atgctgaaat cttcgtcgtt aagttgcaaa ttaagggtat tcccgacgcc
    29221 aaactggctg ggcaattcac gttcaataac cgcaccattg gtgatccgtc caccgttcag
    29281 ttggttcacc tgaacactgc taccgccagc ggaggctcct gcgccgccaa ccagaatatt
    29341 gccctgcgcc agcgcataca cctgactgtc aacgccctta agcggcgtca tcaacaacgt
    29401 acctccacgc aagcttttgg catttcccat ggaagaaacc accacatcga tggtttgccc
    29461 ctgacgtcca aacggaggaa gtgacgctgt caccattacc gcagcgacgt tttttagctg
    29521 catattggtg cccgtcggaa cggtaattcc cagctgtgag agcatgttat taagcgtttg
    29581 tgtggtaaac ggcgtctggg ttgtctggtc accggtgcca tccagcccca ccaccagacc
    29641 atagccaatc agtgagtttt gccttacccc ctgaacactg gtgagatcgc gaatacgctc
    29701 agcctgagcc gccgtcgtga ccagtagaag aattaatgca gagagaaatt taatcaccac
    29761 agcctcactt acattggcga caggttaagg aagaaacgct gcaaccagcc catattttgc
    29821 gcttcgttaa tgtagccatt gcctacgtat tcaatgcgcg catccgccac ctgagtagac
    29881 ggtacggtat tgctgccgct gatagtgcgt ggattaacca cgccagagaa gcgaataaat
    29941 tcggtaccct gattaatggc aatctgtttt tcacccacca catgcaggtt gccgttgacc
    30001 agtacctggt caaccgtcac cgtcaacgtg ccgctaaagg tattgctggc attggccccg
    30061 ccctttccgt tgaacgtgtt accaccggag gcttcgacat cggcacgagc gttaccaaac
    30121 agcccctgca aatagcgcgg cacagtatca aagccaaaat tagttttacc gtcacggctg
    30181 gcattcgcag aggagctttt gctggcgctg acgttctcct gcaacacgat ggtcagcgta
    30241 tcgccaatat tgcgtggtcg acgatcttca aacagcggtt gatagccata gttaatcggc
    30301 tgagcagact ggaaaataga accgttggcg acgggcgtcg gaccgggaac cggttgtgca
    30361 ctggtcgccc cctgcaccag cggcgtggag ggtatccagg cgcagccggt tagtgaaagc
    30421 accaacaagc tggaaatggc ataagtatgc gcagcgtttt tttgcatggc tatcatcttc
    30481 aaaaatcagt aaaccggtga acctgccacc ggttaagcct tagagttgcg tcagtttttg
    30541 cagcatctga tcggtggtgg acaccgcttt actgttgatt tcgtaagcgc gttgcacctg
    30601 aatcatattg accagttctt ccgccacgtt gacgttagac gtttcaacat acccttgata
    30661 cagcagtccc gcgccgttca ggcccggcgt gctttcgttc ggtgcaccag aggattgcgt
    30721 ttcggtgtag aggttttcgc caatgctctc cagcccggtg tcattcataa aggtggtgag
    30781 attgagctgc ccaacctgaa ccggagctgc ctggccttgt tgggttacgc tgaccacgcc
    30841 atcacgaccg atggtgatac ttaacgcatt cgccggaatg gtgatcgctg gctgcacctg
    30901 aaaaccacca gccgtcacca gctgcccgtt ctgatccacc tggaaagagc cgtcacgggt
    30961 ataggctgat gaaccatctg gcaacatcac ctggaaaaag ccctgccctt taatcgcgac
    31021 atctttgctg ttgttggtct gcgacaggtt tccctggctg tgtaagcgtt cagtggcgac
    31081 cgggcgtacc cccgtgccga tttgtaatcc ggagggtaag gtggtttgtt cggaagactg
    31141 tgcccccggc tggcgaatgg tttgataaag cagatcttca aacaccgcgc gctgacgctt
    31201 aaaaccgtta gtactgacgt ttgccaggtt gttggcaatg acgtccatat tggtttgctg
    31261 ggcgtcaagg cccgttttgg cgatccataa tgaactgatc ataaaatgtc ctgtagtgga
    31321 ttagtgacaa gtagagcaga agcacgcgac agtgtgaatt tcccctcacc ctaaccctct
    31381 ccccaaaggg gcgagggaat agtccgcgcg cttttattgc aatgtcatca atcgttcaat
    31441 taactcatcg acagcagttg gttggcacgg cctgcgttat catcgacgct gctgatcacc
    31501 ttcatctgca tttcaaaacg ccgcgcgctg gcaatcatgt cgctcattgc cgcaacggca
    31561 ttgacgttac tgccttccag aacccccgac atcacacgca aggttggatc tgcctgcagt
    31621 atcggcccac gcgtggcctg gctttctgcg cttaaacgaa aaatgccgtc gtcaccgcgt
    31681 tgcacttcgc tgcccgtggc tttcaccagt ttcagacgcc ccactggcgc aaccgtattt
    31741 gccggatcgc ccggattgag cgccgagatt gtgccatcgg cagcaatagt gatttccgcc
    31801 ccttccggca cagcaattgg cccagcctcg cctatcaccg gatgcccctg aattgtcagt
    31861 tgcccggtgg gatcaacctg aatgctgcca ttacgcgtat acccttcgct gccgtcagcg
    31921 gactgcacgg ccagccagcc atcctgctgc aatgcaacgt ccagcgggcg cgaggtgtaa
    31981 tccatcttgc cgggcgtcat gtctgcaccc ggtgttgacg ccgttaccag cgtgcgcgta
    32041 ggcagtgaaa gcccttccac cggcactgca cgcaaggcgt ttaactgtgc gcgaaaaccg
    32101 ggcgttgagg cattggccag attactggcg gttaccgcct gttgattcag cgtctggctg
    32161 gctgctccca tcgcggtata aattgcgtga tccattgagc tatcccgtca gcgattagcg
    32221 taagttaacc agcgtgttga ggatctggtc ctgggttttg atggtctggg cgttagactg
    32281 atagttacgc tgggcaacga tcatattgac cagttcttta ctgagatcga cgttggacgc
    32341 ttccaacgca ccgttggtca gggtgccaaa gttgcccgtc ccggctgtcc ccaacagcgc
    32401 cacgccagaa gattgcgtcg cagaccagac gttgtcgcct tcggatgcca gaccttcgtt
    32461 gttggcaaag ttcgccagta caatctgccc cagcagttgt gtttgttcgt tggaatagtt
    32521 gccgacaacc gtaccgtcat cattgatttg ataactcacc agatcgcccg gtttgtagcc
    32581 attctgggtg gttgccacaa tattgttagc gccggtattt tgctgcatgg agttgaggaa
    32641 gctcaggcta aacgtggcgg gatctgcgcc gttaattgcg ccagtggtaa tattctctgt
    32701 tggatttgag gtcagaacgc cattggcatt aaacaccagc ttcattgcag gctcggctgt
    32761 acctgttgga tcactgctat cctgggtgta gacctgccag ttattatccc cggtcttcac
    32821 aaagtagacg ctcatgtcat gagcattacc ctgactgtcg aaaacagtca ccgaaccttt
    32881 tttgttatag ctatccgcat tgctggcgct gaatggcgta acagtaggaa gcggatcgct
    32941 ggaattcagg ttgatctgca tcgatgccgt ggtggtagtt ttcgctgcca tcagggtatt
    33001 cgggatcgaa atattggtcg gattcgcccc ttgctgaata gtcggcggcg taccggttgc
    33061 cgggtaaccc gtcagctgta aacctttcat attcaccagg ttacggtttt catccagctt
    33121 aaattgtccg ttacggctgt agaacaccga accgttgctg tctaccagac ggaaaaaacc
    33181 gttctggctg atagcaacgt ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa
    33241 gtcctgagtg ataccggcaa cttttacccc cagtcccact ttcgaaccgg caaacatatc
    33301 ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg gagttggcga tattgttgcc
    33361 aataacatcg aggttggtgg cggcagcgtt taatccgcta accgcttgag aaaaggccat
    33421 gactgactcc tgaaatgtga aggcttaaat tatctgccgt acttcgtcga gggtggtggt
    33481 gccgtaagtg ccgagatcca gcgtattacc gctgttgccg cggatcacac cctgcaccag
    33541 agcaaactgc agcggctggg caaccagttg tgtaccaccg ttactggcgc taatcgctac
    33601 attgtaagaa ccgttcggcg cagtgctgcc gtcagtcaac gtaccgtccc aggtgaaact
    33661 gtgaactccg gcggtcagtt caccaatatc aatggtgcgc acaaccgcac catttttatc
    33721 ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt
    33781 cgtggtcaca gctccttctt cactgccggt tcccgcaaga acagtggtgc cggggatcat
    33841 cacgccgtga ccgatcaggt tactggcctg taacgactgg ctgttatcaa tctgtccgga
    33901 aatagatccg agtgtggtat tgagtttttc aatcccgctg accgtgctga tttgtgccaa
    33961 ttgcgacgtc agctcgttgt tttccattgg attggtcggg tcctggtttt tcagctgcgc
    34021 caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact
    34081 actgctggtg gtactgacgc cggtatttgt cggatcggtg gtggttaccg caatggacat
    34141 agctttctcc tttattgacc gagcgtaagg gttttcagca tcatgctttt caccgtgttg
    34201 agcacttcaa cgttggcctg atagctgcgt gacgccgaca tggtgttaac catctccccg
    34261 acgacatcaa cgttcggcat ttttacgtag ccctttgcat ctgccagcgg attacccggt
    34321 tcataaacca gtttgtccgg ggcctgactt tctataacat cggcaacctt tacgccgcct
    34381 gtcgcagcac ctggtgcagc gttaacctgg aataccacct gttttgcccg atatggctgt
    34441 ccatcaggac cggtcacgct atcagcattt gccagattac tggccgccac gttcaggcgc
    34501 tgggactggg cagttaacgc cgacccggcg atatcaaaaa tattcagcag tgccatccgt
    34561 taatttccgc tctgtaaaac gttcatcatg cctttgattt gcccgctcaa cgcgctaagg
    34621 ctcatctggt attgcaggct gttatcggca aactgggtgc gttcgcgatc catatcgacg
    34681 gtattaccgt caagcgaagg ctggtccgga atacggtatt gcagttctgc ggtaggaggc
    34741 gtcagcgcct gcgccgggat gtgttgcgtt gaggtcatcg tcagtgcaac cacactggtt
    34801 gcatcccgtc cacgttgcat gactttttta agttcactgg caaaatcgat atcgcgcgcc
    34861 tgataaccag gggtatcggc attggcgatg tttgctgcca gcacttcctg acgctgggcg
    34921 cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt cgagcttatc gagcatatct
    34981 cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc
    35041 ggaataaacg caaaatgggt cgctatttat gccgttgatg gtcattgcgg acaggtacaa
    35101 ttcacgttgt agaaatggct gggggcgaaa atgctgacaa taaaacgtag cgtggcgatc
    35161 atcgcgatac tgttcagtcc gttaagtacg gcgagcaatc tcacatcgca attgcacaac
    35221 ttttttagcg cccaactcgc gggggtaagt gatgaggttc gtgtttctat tcgtacagcg
    35281 cccaatctac taccgccatg cgagcagcca ttgctttcga tgagcaataa ttcccgcctg
    35341 tggggcaatg tgaatgtgtt ggcacgctgc ggtaacgaca aacgatattt acaggttaat
    35401 gtgcaggcca caggaaatta tgtggttgcc gcgatgccca ttgtgcgggg aggaaagctg
    35461 gaagctggca atgtcaaact aaaacgcgga cggctggata ccctgccacc gcgtacggtg
    35521 ctggatatca atcaacttgt tgatgccatt agcctgcgcg atctatcacc cgatcaacct
    35581 atccagttaa cccagtttcg ccaggcatgg cgggtaaaag cgggacaacg cgtcaatgtg
    35641 atcgccagcg gtgatgggtt tagcgccaac gcagaaggtc aggcgctgaa caatgcagcc
    35701 gtcgcacaga atgcgcgggt gcgcatggta tcgggacagg tagtcagcgg cgttgttgat
    35761 gcagatggga atattcttat aaacctgtaa gctgttaaag attacccgtc ccttgccgat
    35821 aaataagcaa cacatgataa aagcgccctc aatgaggaat aaaccatgag tattgatcgc
    35881 acttcgcctc tgaagcctgt aagcaccgtt caaccgcgcg aaaccactga cgcgccggta
    35941 acgaacagcc gggcggcaaa aacaaccgcc tccaccagca ccagtgtgac gttaagcgac
    36001 gcgcaagcaa aactgatgca acccggcagc agtgatatca atcttgaacg tgtcgaagcg
    36061 ttaaaactgg cgattcgtaa cggtgaacta aaaatggaca ccggcaaaat tgccgatgcg
    36121 ctgatcaacg aagcgcagca agacttgcag agtaactgat cgtatgacac gtcttgcaga
    36181 gatcctcgac cagatgtccg ctgtgcttaa cgatctcaaa acggtaatgg ttcaagagca
    36241 gcaacatctc tctatggggc agatcaacgg cagccagttg caatggatta cagaacaaaa
    36301 aagctcactg ctggcgacgc tggattacct cgaacagtta cgcaagaaag aacccaatac
    36361 agcaaatagc gttgatatta gtcaacgctg gcaggaaatt actgtgaaaa cgcagcaact
    36421 acgccaaatg aatcaacata acggctggtt actggaagga cagattgagc gcaatcaaca
    36481 ggcgctggaa atgttgaaac cgcatcagga accgacgcta tatggggcga acggtcagac
    36541 ctcaacaacc catcgcggcg gtaaaaagat ttcgatctga atattatctc cggcctgcac
    36601 tgcaggccgg aatgcattgt tacaccgtcc ggcgggcaaa ttctttaact ttgaagccca
    36661 gtaccgccag tgcagcgaag tacgcggcaa tccccgccag cacgaccgcc attaaacgca
    36721 gtaagcgcca gggcatggta cccaatgacc actccggcat gatatgtaac atacctaaaa
    36781 gcacgccaga catcaccagt accgccacca ccagacgcaa cagaaacgcc atccagccgg
    36841 gttgcggagt aaagattttc tgcttacgca actgccagta aagcagcgaa gcattcagac
    36901 acgccgccag accaatagaa agtgacagcc cggcatgttt caacggacca ataaacgcca
    36961 ggttcatcaa ttgcgttaaa atcagcgtaa cgatggcaat tttcactggc gttttaatgt
    37021 cctggcggga ataaaagcca ggagccaaca ctttcactac aatcaggcca atcaaaccca
    37081 ccgagtaggc aattaacgcc cgctgggtca tcagcgcatc aaacgcggta aatttaccgt
    37141 actggaacag cgaaacggtt aacggacctg aaagaatgcc caacgcaacc gcactcggca
    37201 gcgccaacag gaaacaaaga cgcaaccccc agtccatcaa acggttgtat tcatcatgat
    37261 tgccactggc aaaacttttc gacagcgacg gcagcaaaat ggtgccaagc gccacgccca
    37321 gcacaccgga cggaaactcc attaagcggt cggcgtaata catccaagac accgaaccgg
    37381 aagcaagaaa cgaggcaaaa atggtgttga tgattaagga gatctggctc acagagacgc
    37441 caaggatcgc cggtcccatc tgtttcacca cgcgcattgc tccggcatcg tggaagttaa
    37501 tgcgcggcag gaccagcatg ccgatcttct tcaggtgcgg tagctgatac accagctgca
    37561 ggatgccgcc gaccgtaaca gcccacgcca gcgccagcac cggtgggtta aagtacggtg
    37621 cggcaaacag cgcgaaacca atcatgctga tgttaagcag tgttggagca aacgccggaa
    37681 tcgagaagcg gttccacgta ttcagaatcg ctcccaccag cgacgccagg gagatcagca
    37741 agatataggg aaaggtaatc tttagtagct ggctggtcag ggcaaatttg tcagctgtgt
    37801 cagcgaagcc tggcgcggtc accatgatca cccacggtgc ggcgagcatg ccagcgaccg
    37861 tcacaaccgc cagcgcgagt gtcagcaggc cagaaacata agagacaaag acccgcgtgg
    37921 cgtcttcacc ctgcttactt ttatattccg ccagaatcgg tacaaatgcc tgggaaaatg
    37981 ccccttcggc aaagatacgg cgtaacaagt taggaagttt aaaagcgacg aaaaaggcgt
    38041 cggttgccat ccctgcgcca aagattctgg cgacaattgc gtctcgtgcg aagccaagca
    38101 cacgcgaaaa catggtcatc gagctgacgg cggccagcga ttttaataaa ttcatcggtg
    38161 ttctaatcca gacccacggg caaaacgcct gcaaggcagg cgctgaaaga ggcgaatagt
    38221 ctacctggat tatggtgaat tgctaccgcc agatgttaca gggtttattc actcatcgca
    38281 tcgcgccaga tcttgtcaac gatacgttgc gccagcacgg cctgttcgcc ggcggtttgc
    38341 ggaactgtct gattttgcac acattcaatg aagtgccgcg cacagccgac aaatccacgt
    38401 tgttcaagag tgctctgcca accaggaatc ggtttatgca ctacgccctg cccgcgctcc
    38461 tcacgccatt cgcgcatatc cgtaatgtcg atgagcgcac cgtcagtcac ggcctgcacg
    38521 gtttcacgct gacttccggc acggcgatgc atacaggtgg tgatctgcaa aggaccggcc
    38581 gaaaaatggt gctcggcaaa cagcatttcg ccagcgtcgt tagtcagtag cgtaccgcca
    38641 tccagagagg ctttgccgcc cgacaaccac agcgcggtat ccaccacatg cagataatca
    38701 tccagcaacg tgaaataaag atcgtgtggc ccgacactat tgctacgatg tttatccatt
    38761 cttagcgaag ctgcggtagc gagttgcgtt tttaactcac cgtagagtgg tgcgaaacga
    38821 cggttaaaac cgaccatcaa cgtcagtttt ttccgcgccg ccagttccac cagccgttca
    38881 gcatcgcgca gattttctgc cagcggttta tcgacacaga catgtacacc cgcattgagt
    38941 aacgtactga ccacgtcaaa gtggctggcg gtgctggaat gcacaaaaac cgcatcgcaa
    39001 ctggcggcaa ggctggataa cgaatcggca taaggaatgc gccagctttc acaaattggc
    39061 agggctttcg cgcgcgtagg cgaccaggct ccttgtaaca tccagtcaga cgctgccgcc
    39121 agcaccggta accacgcttt ttgcgcaatg ccgcctaatc ccacaacgcc gatacgtaat
    39181 tttttcactt taatctccca gatgggccag caacgaatca agacgctgtt tcagttctgc
    39241 cacttcgatt tccagggctt cgacgcgggc ctgtaaatca ccatcaaccg cgttcgacat
    39301 atccgttacc gccggctgat cctctacctc accactgaaa agatgcatgt agcggctttc
    39361 gcgtttaccc ggttcgcggg ccagacgcac cacaaaagga ccatcttcgc gatttgccag
    39421 ttgttccagc gtcgactcca cttccgccat atcgctgaat tcatacattc gcgcggcgcg
    39481 gctgcgcagt tcacctggcg tctgggcacc acgcaataac aacgtggtga ttaacgccac
    39541 ttccgctgcg ctcagtttca gatcgccaaa ttctgaatta caaaaacgtt gctcatattt
    39601 ggtgacccga ttaccaaaac cgctcactgt gcgtagataa tgacgtttga ccagattatc
    39661 cagctgttcc tgcacttcgg attcactcag attcatgacc ggttcacggt tcgttttctg
    39721 attacaggcc gtgactacac cattgactga gagcgggtat tgctccggcg tggtcacctg
    39781 cttttccagc aagcaaccaa tcacgcgagc ttcaagggca gtaagttgat atttcatgag
    39841 attctcctta gcggccgggc gtccagtctg gggtagttaa tgccgtcagt acgtgatcgc
    39901 gccattgtcc atcaatcaac agatagtctt tcgcatagcc ttctttttca aaacccagtc
    39961 gcgccagtaa atcaccgctg cgtttattgt gcggcatata attagccata atgcgatgaa
    40021 tatgttgggt gcgctgcata taacgaatgg ctgcggtcag ggcttcaaac atgagtcctt
    40081 tgccctgcca tttttgccca atcgaataac cgagatagca ggcatgaaaa gagccacgaa
    40141 caacattgga aaaattggca acgccaataa tctctttttc gtccgggtcg aataagccaa
    40201 agtagaaagc tgaaccttgt ttatgaaatt cgttaatcat ccccagcctg gcctgccagc
    40261 ctgatggata acagtggctt tcgtcgcgca ctggctccca gggcttgagg aaatggcgat
    40321 tctctgcgta ataatccgca agacgccagg catcacgatc atgcaccaga cgcacgacca
    40381 gtcggtctgt ggttaagcgc acttttggca cgttactgcg atagccaaac attctatacc
    40441 tactccttcc cgtaacgtct ttaatacgct gtaagtatta ctatacctgc gcaaccagac
    40501 cctgtgaaaa gtccaaaatg ccattttctg cattattcac atatttgata aaaactctct
    40561 atcaaagcga cgttttgata aaaaaatatt gtcccctaag tgatgggaaa agtcttagcg
    40621 gcaatgcaga atagaaagtg ctcatctttt atttcccggg aggggaaatg tcccgcgtat
    40681 cgcaggcgag gaacctgggt aaatatttcc tgctcatcga taatatgctg gtcgtgctgg
    40741 ggttctttgt tgtcttcccg ctgatctcta tccgcttcgt tgatcaaatg ggctgggccg
    40801 ccgtcatggt cggtattgct ctcggtctac gccagtttat tcagcaaggt ctgggtattt
    40861 tcggcggcgc aattgccgac cgctttggtg ccaaaccgat gattgttacc ggtatgctga
    40921 tgcgcgccgc cggattcgcc acaatgggta tcgcccacga accgtggcta ttgtggtttt
    40981 catgcctgct ctcgggactc ggcggcacgt tgtttgatcc gccgcgttcg gcgctggtgg
    41041 tgaaactaat ccgtccacaa caacgtggtc gttttttctc gctgttgatg atgcaggaca
    41101 gtgccggtgc ggtcattggc gcattgttgg ggagctggct gttgcaatac gactttcgcc
    41161 tggtctgcgc cacaggggcc gttttgtttg tgctgtgtgc ggcgttcaat gcgtggttgt
    41221 taccggcatg gaaactctcc accgtacaca cgcccgttcg cgaaggcatg acccgcgtga
    41281 tgcgtgacaa gcgttttgtc acctatgtcc tgacgctggc gggttactac atgctggctg
    41341 tacaagtgat gctgatgctg ccaattatgg tcaacgacgt ggctggcgca ccctctgccg
    41401 ttaaatggat gtatgccatt gaagcgtgtc tgtcgttaac gttgctctac cctatcgccc
    41461 gctggagtga aaagcatttt cgtctggaac accggttgat ggctgggctg ttgataatgt
    41521 cattaagcat gatgccggtg ggcatggtca gcggcctgca acaacttttc accctgattt
    41581 gtctgtttta tatcgggtcg atcattgccg agcctgcgcg tgaaacctta agtgcttcgc
    41641 tggcggacgc aagagctcgc ggcagctata tggggtttag ccgtctgggt ctggcgattg
    41701 gcggcgctat tggttatatc ggtggtggct ggctgtttga cctgggcaaa tcggcgcacc
    41761 agccagagct tccgtggatg atgctgggaa ttattggcat cttcactttc cttgcgctgg
    41821 gttggcagtt tagtcagaaa cgcgccgcgc gtcgtttgct tgaacgcgac gcctgatttg
    41881 atactccccc ctgactcctc catactgaaa gtgaactgac agccaaatgg aggagtcacg
    41941 tgaagctata catttacgat cactgccctt actgcctcaa agcccgcatg attttcggcc
    42001 tgaaaaatat ccccgtcgaa ttacatgttc tgctcaacga cgacgcagaa acacccaccc
    42061 ggatggtcgg tcaaaaacag gttcccattc tgcaaaaaga tgacagccgc tatatgccag
    42121 aaagcatgga catcgttcac tatgtcgata aactcgacgg caaaccgtta ctgaccggca
    42181 aacgttcccc tgccattgaa gagtggctgc gcaaggtcaa tggctacgcc aacaaactgc
    42241 tgttgccgcg ttttgccaaa tcggcattcg atgagttttc tactcccgcc gcgcgcaaat
    42301 atttcgtcga caagaaagag gccagcgcgg gtaattttgc cgacctgctg gcccactctg
    42361 acggtctgat taagaatatc agcgatgatt tacgtgctct ggacaaactg atcgtcaaac
    42421 cgaacgccgt gaatggcgaa ctttcggaag atgatattca gctatttccg ctgctgcgta
    42481 atctgacgct ggtagccgga atcaactggc caagccgcgt tgctgattac cgcgataata
    42541 tggcgaaaca gacacaaatc aatttgttat catcaatggc gatttaatcc tgacccgccg
    42601 cggcagcctg tattcgcggg ttgccgccgt tttcagtttt ttctgctggc gagtttatcg
    42661 ctggtgacac atgctgcgtt gtgtcaggat attgacgcag atttcatgtc gaggactacc
    42721 atgaacaagt ttttatttgc cgctgcattg atcgtcagtg gcctgctcgt tggctgtaat
    42781 caactcaccc aatacaccat caccgaacaa gaaattaacc agtcgcttgc gaaacataat
    42841 aatttctcaa aagatatcgg tttacccggc gtggctgacg cccatattgt tctgacaaac
    42901 ttgaccagcc aaattggtcg cgaagagccg aataaggtta ccctaaccgg agacgccaat
    42961 ctggacatga actccctgtt cggtagtcag aaagcgacca tgaaactgaa gctgaaagcg
    43021 ctgcccgtgt ttgataaaga aaaaggtgcg atcttcctga aagagatgga agtggtcgat
    43081 gcgacggtac aaccggaaaa aatgcaaacg gtgatgcaaa cgttgcttcc ctatttgaac
    43141 caggcattac gcaattactt taaccagcaa cctgcttacg tcctgcgcga agatggcagt
    43201 cagggcgaag caatggcgaa aaaactggcg aaaggcattg aagtgaagcc aggcgaaatt
    43261 gtcattccgt ttacggatta atgacgaggg cgcattcgcg ccctttattt ttcgtgcaaa
    43321 ggaaaacgtt tccgcttatc ctttgtgtcc ggcaaaaaca tcccttcagc cggagcatag
    43381 agattaatga ctgcaccatc ccaggtatta aagatccgcc gcccagacga ctggcacctt
    43441 cacctccgcg atggcgacat gttaaaaact gtcgtgccgt ataccagcga aatttatgga
    43501 cgggctattg taatgcccaa tctggctccg cccgtgacca ctgttgaggc taccgtggcg
    43561 tatcgccagc gtattcttga tgccgtacct gccgggcaca atttcacccc attgatgacc
    43621 tgttatttaa cagattcgct ggatcctaat gagctggagc gcggatttaa cgaaggcgtg
    43681 ttcaccgctg caaaacttta tccggcaaac gcaaccacta actccagcca cggcgtgacg
    43741 tcaattgacg caatcatgcc ggtacttgag cgcatggaaa aaatcggtat gccgctactg
    43801 gtgcatggtg aagtgacaca tgcagatatc gacatttttg atcgtgaagc gcgctttata
    43861 gaaagcgtga tggaacctct gcgccagcgc ctgactgcgc tgaaagtcgt ttttgagcac
    43921 atcaccacca aagatgctgc cgactatgtc cgtgacggaa atgaacggct ggctgccacc
    43981 atcactccgc agcatctgat gtttaaccgc aaccatatgc tggttggtgg cgtgcgtccg
    44041 cacctgtatt gtttacccat cctcaaacgc aatattcacc aacaggcatt gcgtgaactg
    44101 gtcgccagcg gttttaatcg agtattcctc ggtacggatt ctgcgccaca tgcacgtcat
    44161 cgcaaagaga gcagttgcgg ctgcgcgggc tgcttcaacg ccccaaccgc gctgggcagt
    44221 tacgctaccg tctttgaaga gatgaatgct ctgcagcact ttgaagcatt ctgttctgta
    44281 aacggcccgc agttctatgg cttgccggtc aacgacacat tcatcgaact ggtacgtgaa
    44341 gagcaacagg ttgctgaaag catcgcactg actgatgaca ccctggtgcc attcctcgcc
    44401 ggggaaacgg tacgctggtc cgttaaacaa taaaaatcgt agcttcctgt tgtcattagg
    44461 ttattttgcc tgtataaata accagtatat tcaacagggg gctattatgc gaattgaagt
    44521 caccatagcg aaaacttctc cattgccagc tggggctatt gacgccctgg ctggcgaact
    44581 ttcccgccgt attcagtatg cgtttcctga taatgaaggc cacgtatcgg tacgttatgc
    44641 cgcagcgaat aatttatcgg ttattggcgc aacaaaagaa gataaacagc gcattagcga
    44701 aattctccag gaaacgtggg aaagcgccga tgactggttt gtcagcgaat aatatgcagt
    44761 gatttttgtt tttgccgggt cgccccggct tttttatttt acttacaaat tttgagtaca
    44821 aaatttctac acttcctcaa aataattgca caatatgcta aaaaaatatg aatgacatgc
    44881 tgttttattg
//
LOCUS       DAZFRN010000019        44762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFRN010000019 DAZFRN010000000
VERSION     DAZFRN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.019"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(40..1452)
                     /gene="pykF"
                     /locus_tag="ACHKUB_001044"
     CDS             complement(40..1452)
                     /gene="pykF"
                     /locus_tag="ACHKUB_001044"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO6654093.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1460..1654)
                     /locus_tag="ACHKUB_001045"
     CDS             complement(1460..1654)
                     /locus_tag="ACHKUB_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654094.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2009..2218
                     /gene="fumD"
                     /locus_tag="ACHKUB_001046"
     CDS             2009..2218
                     /gene="fumD"
                     /locus_tag="ACHKUB_001046"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO6654095.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            2674..3300
                     /locus_tag="ACHKUB_001047"
     CDS             2674..3300
                     /locus_tag="ACHKUB_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO6654096.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            3321..5423
                     /locus_tag="ACHKUB_001048"
     CDS             3321..5423
                     /locus_tag="ACHKUB_001048"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO6654097.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            5436..6074
                     /locus_tag="ACHKUB_001049"
     CDS             5436..6074
                     /locus_tag="ACHKUB_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO6654098.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            6138..6806
                     /locus_tag="ACHKUB_001050"
     CDS             6138..6806
                     /locus_tag="ACHKUB_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6654099.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            6803..7588
                     /gene="phsC"
                     /locus_tag="ACHKUB_001051"
     CDS             6803..7588
                     /gene="phsC"
                     /locus_tag="ACHKUB_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO6654100.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            7592..8404
                     /gene="ydhT"
                     /locus_tag="ACHKUB_001052"
     CDS             7592..8404
                     /gene="ydhT"
                     /locus_tag="ACHKUB_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO6654101.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(8416..10020)
                     /locus_tag="ACHKUB_001053"
     CDS             complement(8416..10020)
                     /locus_tag="ACHKUB_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO6654102.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLVVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(10146..10451)
                     /locus_tag="ACHKUB_001054"
     CDS             complement(10146..10451)
                     /locus_tag="ACHKUB_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO6654103.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(10559..10635)
                     /locus_tag="ACHKUB_001055"
     tRNA            complement(10559..10635)
                     /locus_tag="ACHKUB_001055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10599..10601),aa:Val,seq:gac)
     gene            complement(10640..10716)
                     /locus_tag="ACHKUB_001056"
     tRNA            complement(10640..10716)
                     /locus_tag="ACHKUB_001056"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10680..10682),aa:Val,seq:gac)
     gene            11024..12280
                     /locus_tag="ACHKUB_001057"
     CDS             11024..12280
                     /locus_tag="ACHKUB_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6654104.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(12321..13694)
                     /gene="mdtK"
                     /locus_tag="ACHKUB_001058"
     CDS             complement(12321..13694)
                     /gene="mdtK"
                     /locus_tag="ACHKUB_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO6654105.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            13909..14550
                     /gene="ribE"
                     /locus_tag="ACHKUB_001059"
     CDS             13909..14550
                     /gene="ribE"
                     /locus_tag="ACHKUB_001059"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO6654106.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(14590..15738)
                     /gene="cfa"
                     /locus_tag="ACHKUB_001060"
     CDS             complement(14590..15738)
                     /gene="cfa"
                     /locus_tag="ACHKUB_001060"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO6654107.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(16029..17240)
                     /gene="punC"
                     /locus_tag="ACHKUB_001061"
     CDS             complement(16029..17240)
                     /gene="punC"
                     /locus_tag="ACHKUB_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO6654108.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            17353..18285
                     /gene="punR"
                     /locus_tag="ACHKUB_001062"
     CDS             17353..18285
                     /gene="punR"
                     /locus_tag="ACHKUB_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO6654109.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(18282..19307)
                     /gene="purR"
                     /locus_tag="ACHKUB_001063"
     CDS             complement(18282..19307)
                     /gene="purR"
                     /locus_tag="ACHKUB_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO6654110.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            19606..19695
                     /gene="cydH"
                     /locus_tag="ACHKUB_001064"
                     /gene_synonym="ynhF"
     CDS             19606..19695
                     /gene="cydH"
                     /locus_tag="ACHKUB_001064"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO6654111.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            19861..21030
                     /locus_tag="ACHKUB_001065"
     CDS             19861..21030
                     /locus_tag="ACHKUB_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6654112.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(21176..21757)
                     /gene="sodB"
                     /locus_tag="ACHKUB_001066"
     CDS             complement(21176..21757)
                     /gene="sodB"
                     /locus_tag="ACHKUB_001066"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO6654113.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21885..22700)
                     /gene="mepH"
                     /locus_tag="ACHKUB_001067"
     CDS             complement(21885..22700)
                     /gene="mepH"
                     /locus_tag="ACHKUB_001067"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO6654114.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            23034..23381
                     /gene="grxD"
                     /locus_tag="ACHKUB_001068"
     CDS             23034..23381
                     /gene="grxD"
                     /locus_tag="ACHKUB_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO6654115.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(23432..28048)
                     /locus_tag="ACHKUB_001069"
     CDS             complement(23432..28048)
                     /locus_tag="ACHKUB_001069"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO6654116.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNTTGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAITGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(28141..28788)
                     /gene="rnt"
                     /locus_tag="ACHKUB_001070"
     CDS             complement(28141..28788)
                     /gene="rnt"
                     /locus_tag="ACHKUB_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO6654117.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(28891..29298)
                     /gene="gloA"
                     /locus_tag="ACHKUB_001071"
     CDS             complement(28891..29298)
                     /gene="gloA"
                     /locus_tag="ACHKUB_001071"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO6654118.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(29379..30476)
                     /gene="nemA"
                     /locus_tag="ACHKUB_001072"
     CDS             complement(29379..30476)
                     /gene="nemA"
                     /locus_tag="ACHKUB_001072"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO6654119.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(30513..31112)
                     /gene="nemR"
                     /locus_tag="ACHKUB_001073"
     CDS             complement(30513..31112)
                     /gene="nemR"
                     /locus_tag="ACHKUB_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO6654120.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFVPLENALAHVKNIIATPAV"
     gene            31215..31454
                     /locus_tag="ACHKUB_001074"
     CDS             31215..31454
                     /locus_tag="ACHKUB_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO6654121.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            31503..32399
                     /locus_tag="ACHKUB_001075"
     CDS             31503..32399
                     /locus_tag="ACHKUB_001075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO6654122.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            32480..33001
                     /gene="sodC"
                     /locus_tag="ACHKUB_001076"
     CDS             32480..33001
                     /gene="sodC"
                     /locus_tag="ACHKUB_001076"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO6654123.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGECYACGVIK"
     gene            complement(33002..35014)
                     /locus_tag="ACHKUB_001077"
     CDS             complement(33002..35014)
                     /locus_tag="ACHKUB_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6654124.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(35014..35871)
                     /locus_tag="ACHKUB_001078"
     CDS             complement(35014..35871)
                     /locus_tag="ACHKUB_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO6654125.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(35874..36110)
                     /locus_tag="ACHKUB_001079"
     CDS             complement(35874..36110)
                     /locus_tag="ACHKUB_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO6654126.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            36311..36745
                     /gene="slyA"
                     /locus_tag="ACHKUB_001080"
     CDS             36311..36745
                     /gene="slyA"
                     /locus_tag="ACHKUB_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO6654127.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(36792..37259)
                     /gene="slyB"
                     /locus_tag="ACHKUB_001081"
     CDS             complement(36792..37259)
                     /gene="slyB"
                     /locus_tag="ACHKUB_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO6654128.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTVSEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            37533..38642
                     /gene="anmK"
                     /locus_tag="ACHKUB_001082"
     CDS             37533..38642
                     /gene="anmK"
                     /locus_tag="ACHKUB_001082"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO6654129.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVITTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            38740..39069
                     /gene="mliC"
                     /locus_tag="ACHKUB_001083"
     CDS             38740..39069
                     /gene="mliC"
                     /locus_tag="ACHKUB_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO6654130.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            39128..39784
                     /gene="pdxH"
                     /locus_tag="ACHKUB_001084"
     CDS             39128..39784
                     /gene="pdxH"
                     /locus_tag="ACHKUB_001084"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO6654131.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            39913..41187
                     /gene="tyrS"
                     /locus_tag="ACHKUB_001085"
     CDS             39913..41187
                     /gene="tyrS"
                     /locus_tag="ACHKUB_001085"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO6654132.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            41246..42109
                     /gene="pdxY"
                     /locus_tag="ACHKUB_001086"
     CDS             41246..42109
                     /gene="pdxY"
                     /locus_tag="ACHKUB_001086"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO6654133.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(42153..42758)
                     /gene="gstA"
                     /locus_tag="ACHKUB_001087"
     CDS             complement(42153..42758)
                     /gene="gstA"
                     /locus_tag="ACHKUB_001087"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO6654134.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(42864..44366)
                     /gene="dtpA"
                     /locus_tag="ACHKUB_001088"
     CDS             complement(42864..44366)
                     /gene="dtpA"
                     /locus_tag="ACHKUB_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO6654135.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT        11319 a        10929 c        11653 g        10861 t
ORIGIN      
        1 agggcgcttc gatatacaaa ttaattcaca aaagcaatat tacaggacgt gaacagatgc
       61 ggtgttagta gtgccgctcg gtaccagtgc accagaaacc ataactacaa cgtcaccttt
      121 gtgtgccaga ccgctctgca gagccagttc tttacccaga cggtagaaat catcagtaga
      181 agtgatctct ttaaccagct gcggcacaac gcctttgctc agtaccaact gatgagccgt
      241 tttttcgttg gtggtcagtg ccaggatggt ggcatccggg aagtatttac gtactgcgcg
      301 agcagattta ccgccctggg tagcaaccac gatcagcgga gcatccagtt tttcagcagt
      361 ttcaacggca ccacggcata ccgcttcggt aatgcgcagt ttacggttgt cattgttgaa
      421 ctcgagacgg ctgttcatca cgcggtcggt acgttcgcag atggtcgcca tgatagaaac
      481 cgcttccagc gggtatttac cttttgcgga ttcaccagac agcatcactg cgtcagtacc
      541 gtcgaggatg gcgtttgcaa cgtcaccggc ttctgcgcga gtcgggcgtg ggtttttgat
      601 catggaatcc agcatctggg tcgcagtgat aacgacttta cgtgcacgga tacatttttc
      661 gatcatcatc ttctgggcga agataacttc ttctaccggg atttctacac ccaggtcgcc
      721 acgcgcaacc atgatgccgt cagaggcttc gaggatttcg tcgaagttgt tgaggccttc
      781 ctggttttcg attttggaga tgatgtggat gttttcgccg ccgtgcgctt tcaggtgctc
      841 acggatttcg ataacgtcag aacgcttacg aataaaggaa gcagcaacaa agtctacgcc
      901 ttgttcgcaa ccaaagatca ggtcctgttt gtctttttca gccagtgctg gcagagcaat
      961 ggaaacgcca ggcaggttca cacctttgtt ttcgcccagg tcgccgttgt tcagtacttt
     1021 acagataact ttgttacctt caatggcggt aacttccata ccgatcagac catcgtcaac
     1081 cagtacggtg ttgccaacag acaggtcagt agtgaaacct tcatacgtta ccgcaaccat
     1141 ttcgctgttg ccgataacag atttatcagt ggtgaaagta aaggtctgac cagctttcag
     1201 agaaacgtcg ttaccgcctt ccagtttcat ggtgcggatt tccggacctt tggtatcaag
     1261 caggatagcg gcggttttac cagttttgct catcacgttg cgcagattct gaatgcgctg
     1321 accgtgttct gcatagtcac catgagagaa gttcagacgc ataacgttca tgccagcgtc
     1381 cagcatttta gctaacatct cttcagattc ggttttcggt ccgatggtgc aaacaatttt
     1441 ggtctttttc atgacagtct tagtctttaa gttgagaagg atgggagaaa cttgctttct
     1501 gggcgcactg ccggaaagcg aatccggtat tacgaaagtg gtggtgcctc gctctaagga
     1561 taggtgacat cgaaaaagcg tgcagaggaa tgtgtgcttg tgtctcagcc caacgtgagt
     1621 gcctgttttt gagtcgttga gttctacagt ccaaagtgct gaaaccattc aagagtcaat
     1681 tggcgcgcat tatacgctga aattaatcca aaaggaaaac aaaaacagcg tttcaaaaaa
     1741 tatacaactt gttagacaat aaattgttat caaagattta acgttccagg gaaaaggtta
     1801 cgcaaaaact tcgctgtttg cgcaacgtct ggtgccaaag ttgtcagtgt gaggataagt
     1861 gaaagttcat ccggtgtata aatatatcat cgctcaattg ctatagttaa aataatttat
     1921 cgcgttattt gtctgatttt gtttgatttt tgtttaattg gttagattga tatatgctga
     1981 tagttggcat gataaatagg tataaagaat gggaaatcgg acaaaagagg atgaactgta
     2041 ccgagaaatg tgcagagttg tcggtaaagt tgtgctggaa atgcgcgatc tggggcagga
     2101 accaaagcat attgttatcg caggcgtatt acgtacagca ttagccaata aacgcattca
     2161 gcgcagtgag ctggaaaagc aggcgatgga aaccgtcatt aacgcactgg taaaatgacc
     2221 attcaggatg aggcggtaag cttcatcctg atattcttgc ttcagttgcg gtccagttaa
     2281 tcattctaaa taacaactgc tgaaattata cgaatcgcgg tatgtatttt aatttataac
     2341 gataatatta tttttatttt ctgcaatgaa cgtcacaaat aattgtcgct taagttaaat
     2401 ttatttcaaa tgataataaa tatcagttca cttataattt cttgctttgt aattttaatt
     2461 gataacgatc aatgttaagg gctcagaata atcacattat tttttctcct aaccataaag
     2521 gattagttta tcggcgttaa atcttttctc atacactatc gggttaggga aaatatctgg
     2581 tgttggggac agagtcagca gcgtattagt tgaatgctta atatcaggct gctgaattgt
     2641 cagcactgtg tttgcgaaga ggatgcgaaa taaatgaacc cggttgatcg tccactatta
     2701 gatattggct taacgcgatt agaattcttg cgtatatcag gaaaaggcct tgctggtcta
     2761 acaattgccc ctgcattact gtcacttttg ggttgcaaac aggaagatat tgatagcggc
     2821 acagtagggt tgataaatac acccaaaggg gtgctggtga cccagcgagc gcgatgcacg
     2881 ggttgccacc gctgtgaaat ctcttgtacc aacttcaacg atggctcagt agggacattc
     2941 ttctcccgta tcaaaatcca ccgcaattat ttctttggcg acaacggggt tggctctggc
     3001 ggcggcctgt atggcgatct caactatacc gcggacacct gccgtcaatg caaagagccg
     3061 caatgcatga acgtctgccc gattggtgcg attacctggc agcagaaaga aggctgtatt
     3121 accgtcgatc ataaacgctg tattggctgt agcgcctgta ctacggcatg cccgtggatg
     3181 atggccaccg taaataccga aagtaaaaaa tcctcgaaat gtgtgttatg cggtgaatgc
     3241 gcaaacgcct gcccaacagg ggcgttaaaa attatcgagt ggaaagatat tactgtgtga
     3301 atcttgcaaa ggaaaaaatc atggctaacg gttggacagg taatatatta agagtcaatc
     3361 tcacgacagg aaatattacc ctcgaagatt ccagtaagtt taaaagtttt gtcggtggca
     3421 tgggcttcgg ctacaaaatt atgtatgacg aagtaccgcc aggcacgaaa cctttcgatg
     3481 aagcgaataa attagtcttt gctaccggcc cattaactgg atctggtgcc ccctgtagtt
     3541 ctcgcgtaaa tatcacctca ctttctactt ttaccaaagg aaatttagtt gtcgatgccc
     3601 atatgggtgg cttttttgca gcgcaaatga aattcgctgg atacgacgtc attattatcg
     3661 aagggaaggc gaaatcaccg gtatggctga agattaaaga tgacaaagtt agcctggaaa
     3721 aagccgattt cttatgggga aaagggacgc gcgcaacgac ggaagaaatt tgtcgattga
     3781 ccagtccgga aacctgtgtg gcggctattg gtcaggctgg ggaaaacctt gttcctctct
     3841 ctggcatgtt gaatagccgt aaccacagcg gcggtgcggg aactggcgca attatgggtt
     3901 cgaaaaacct gaaagcgatt gcggttgaag ggacgaaagg ggtcaacatt gccgatcgtc
     3961 aggagatgaa gcgtctcaac gattacatga tgactgaact tattggtgcg aataacaacc
     4021 atgtcgtgcc aagtacgccg caatcgtggg cagagtattc agatcccaag tcacgttgga
     4081 cagcacgtaa agggctgttt tggggcgcgg ctgaaggtgg tccgattgaa acgggggaaa
     4141 ttccgccagg caatcagaac acggtcggct ttcgtaccta taaatccgtt tttgacttag
     4201 gtccggcggc agagaaatac acagtaaaaa tgagtggctg ccactcttgc ccgatccgtt
     4261 gtatgaccca aatgaatatt cctcgggtga aagagtttgg cgtgcccagc acaggtggta
     4321 acacttgtgt agccaacttt gtccatacca ccatcttccc gaacgggccg aaagattttg
     4381 aagataaaga cgatggtcgt gtgattggta acctggttgg tctgaatttg ttcgatgact
     4441 acggcctatg gtgtaactac gggcagctgc atcgcgactt tacttattgt tacagcaaag
     4501 gggtgttcaa gcgtgttctg ccagctgaag agtatgcgga aattcactgg gatcaactgg
     4561 aagcgggtga cgttaatttc attaaagatt tttactaccg tctggcgcat cgtgtgggtg
     4621 agctgagtca cctggctgat ggttcatatg ccatcgcaga acgctggaat ttgggtgaag
     4681 agtactgggg ctacgcaaaa aataaactct ggtcgccgtt tggctatccg gttcaccatg
     4741 ccaatgaagc gtcagcgcag gtcggttcca ttgttaactg tatgttcaac cgtgactgca
     4801 tgacgcatac ccatatcaat tttattggtt cgggtttgcc attgaaactg cagcgtgaag
     4861 tggcgaaaga gctttttggt tctgaagatg cctacgatga aaccaaaaac tacacgccaa
     4921 tcaatgacgc aaaaattaaa tatgcaaagt ggtcgctgtt gcgggtctgt ttgcataacg
     4981 ccgtcaccct gtgcaactgg gtctggccaa tgaccgtttc gccgctgaaa agccgtaatt
     5041 accggggcga tctggcgctt gaagccaaat tcttcaaagc gatcaccggc gaagagatga
     5101 ctcaggaaaa attagattta gctgcagagc gtatctttac gttgcatcgt gcctacacgg
     5161 taaaactgat gcaaaccaaa gatatgcgta atgaacacga tcttatctgt tcctgggtat
     5221 tcgacaagga tccgcagatc ccggtcttta ctgaaggtac tgacaaaatg gatcgtgacg
     5281 atatgcatgc ttcgctcacg atgttctaca aagaaatggg ctgggaccca cagcttggtt
     5341 gtccgacccg cgaaacattg cagcgtctgg ggctggaaga tattgccgcc gatctggcag
     5401 cacacaatct actgcctgcg taaggaatgg gtgagatgaa ccatcgggat gaattgccgt
     5461 tagcgaaagt cagcgaagtt gacgaggcga agcggcaatg gttacagggg atgcgtcacc
     5521 ccgttgatac ggtaaccgaa ccagaaccgg ctgaaatcct ggcagagttc attcgccagc
     5581 attctgtggc tgggcagctg gtggcgcgcg ccgtgtttct ctcgccaccc tattcggttg
     5641 cagaagagga actgtcagtt ttgctggaaa gcattaagca gaacggagac tacgctgata
     5701 tcgcttgtat gacgggaagc caggatgatt actattactc aacgcaggcc atgagtgaaa
     5761 actacgcggc gatgtctttg caagttgtgg agcaggacat ttgtcgcgcc attgcacacg
     5821 ccgttcgttt tgagtgccag acatatcctc gtccttacaa agtcgccatg ctgatgcaag
     5881 cgccatatta cttccaggaa gcgcagattg aagccgcgat cgccgcgatg gatgttgcac
     5941 ctgaatatgc cgatatacgg caggtggagt catcgacggc ggtgttgtac ttgtttagcg
     6001 agcgatttat gacctatggc aaggcttatg gcttatgcga atggttcgag gtagagcagt
     6061 ttcagaaccc ctgaaatttc agggagatgt catggatagg gtggacagtt gcagccactg
     6121 cattagggga taatcagatg tccttcactc gacgcaaatt tgttctgggg atgggaacag
     6181 taatcttttt tactggttct gcgtcttctc tgttagcgaa cacgaggcaa gaaaaggaag
     6241 ttcggtatgc catgattcat gacgagtcac ggtgcaatgg ctgtaatatt tgcgcccgcg
     6301 catgccgtaa aactaaccac gtccccgctc agggaagccg tttatcgata gcacacattc
     6361 cagtcaccga taacgacaac gaaacgcagt atcacttttt tcgccagtca tgtcagcact
     6421 gcgaagatgc tccgtgcatc gacgtatgcc cgacaggggc gtcgtggcgt gatgagcagg
     6481 ggatcgtgcg ggtagaaaaa tcgcagtgca tcggctgcag ttattgcatt ggcgcttgtc
     6541 cgtaccaggt tcgctacctt aaccctgtca ctaaagtggc agataaatgt gatttctgcg
     6601 ctgagtcccg actggcaaaa ggctttccgc ccatttgcgt cagcgcctgc ccggaacacg
     6661 cattgatctt tggtcgcgaa gatagcccag aaattcaggc gtggttgcag caaaataaat
     6721 actatcaata tcagctacct ggtgccggga aaccgcatct gtatcgccgg ttcggtcaac
     6781 atttgattaa aaaggaaaat gtatgaaccc gtcgcaacat gctgaacagt ttcagagcca
     6841 gttagcgaac tatgtgccac agttcactcc cgaattttgg ccggtgtggt tgatcattgc
     6901 cggagtattg ctggttggga tgtggttggt gctggggctg catgccttgc ttcgtgctcg
     6961 tggcgtgaag aaatcagtca ccgattatgg tgagaagatt tatctttact gcaaagcggt
     7021 cagattatgg cactggtcga atgcgttact ctttgtattg ttgctggcaa gtgggctgat
     7081 aaatcacttt gcgctggtgg gcgcaactgc ggttaaaagt ctggttgcgg tgcatgaagt
     7141 ttgcggattt ttgttactgg catgctggct cggctttgtg ctgatcaatg ccgttggggg
     7201 taatggtcac cactatcgca ttcgtcgtca ggggtggctg gagcgagcgg caaaacaaac
     7261 gcgattttat ttgtttggca ttatgcaggg ggaagaacat cctttcccgg caacaaccca
     7321 gtctaaattt aatcccttac agcaggtcgc ctatgttggt gttatgtatg gattgctgcc
     7381 gttgttacta ttgacggggc tgctgtgtct ctatccgcaa gccgtgggag atgtgtttcc
     7441 tggcgtaaga tactggttat tgcaggcgca ttttgctctg gcatttataa gcctcttttt
     7501 tatcttcggt catctttatc tttgcaccac ggggcgtacg ccacacgaaa cctttaaaag
     7561 catggtcgat ggctatcacc ggcactaacg aatgatcatt acacgagcag acctgcgaga
     7621 atggcgtatc ggagcggtga tgtatcgctg gttcctgcgc catttcccac gcggcggtag
     7681 ttatgccgat attcatcatg cactgattga ggaaggatat acggactggg cggagagtct
     7741 ggttgagtat gcgtggaaaa aatggctggc ggatgaaaac ttcgcgcatc aggaagtttc
     7801 ctctatgcaa aaactggcca cagaccccgg agagatacca ttttgcagtc agtttgcccg
     7861 cagcgacgat catgctcgca tcggctgttg tgaggataac gcccgaatcg caaccgctgg
     7921 gtatgccgcg caaattgcca gtatgggtta tagcgtgcgg attggaagtg tcggctttaa
     7981 cagtcacatt ggcagctccg gtgaacgcgc acgggttgct gtaaccggta attcttcgcg
     8041 gataagcagc gctggagata gtagtcggat tgcgaatacc ggaatgcgag tacgcgtttg
     8101 tacattgggt gaacggtgtc atgtcgccag taatggtgac ctggtgcaaa tcgccagctt
     8161 tggcgcaaac gccagaattg ccaacagcgg cgacaatgta cacatcattg ccagcggtga
     8221 aaattcaacg gtagtgagca cgggcgtggt cgactcaatt attctcgggc cagggggaag
     8281 cgccgcactg gcatatcatg acggcgaaag agttcgcttc gccgtcgcca ttgaaggtga
     8341 aaacaatatt cgaaccggag tccgctatcg tcttaatgaa cagcatcagt ttgttgagtg
     8401 ctgatagctt tacctttaat caaacgaaag acgccgacga gcacgggatg caggctttac
     8461 gaccgcccga gccatcgcct caccaatttc tgcacatgcc gtaagtccct gaacgaaagg
     8521 ctggtcgtgc atcaaccagg gcaacgcgcc gaacgctacg cgtccacgaa tatcttcggg
     8581 ttgctgtaac gtataatctt cgccaacatc agggatttca tcccctgttt tctgtaattg
     8641 ctcgcgtagc ccagggaaag gaatatcttt cactttaagc ggacgctgtc cgcgggcatc
     8701 aataaaaacg tcaaacgagt agctgttgtc ttccgttttc aggacggtgc gcgactcatt
     8761 aatttccatt ttgtagtctt caccgagggc gagaatatga atgattcccg cttcacgtaa
     8821 ggcaagtagg cgacgaatag actctgaagg gattgccgca taattatcga tgaaaaccct
     8881 ggcaaggcct ttactgaacc gtttatggtc gtgttcattc agatgtggaa caatttcctg
     8941 tacggcttca tgcaggcgca gaatgacata acgccaggga acagtatgtt tttctcgttt
     9001 attgcgttcg acttcctgga gatttttttc tgcccagtca aatgggtcgc gttgtttgcg
     9061 ctcggcaaac caggcttgag caaaggaatc gacattcagg ctctctaagg ctatgcgttg
     9121 gctccagtct ggatcagcaa acttgatttc ctctactatc aatctaaata cccgatccaa
     9181 aaggccctct tcaccttttt gaatctcagc atttaatgcc tgatcggtga cgatgtgtaa
     9241 gggctcgtag ggaatagggc aatagaaatc ggcttcgggt aaaatacccg tgcgcgacat
     9301 caacgtaata tttagctttt cacttgcgtt atcgcggtta aaaacgacgt gttgtttatc
     9361 atcttcaatg aacgaaccat gctgaatagc cactgccatt gccgcatcca gtccgctcaa
     9421 ggatgttccc ataataccca cgttacacgc atcgaccttt gcttccatca ggcctgacca
     9481 cggactggga aaatacgttc tggttgcttc ttcttcatca ggccagacgt gacccgtggc
     9541 aatcaccact aaatcaaacg tctcgctggg taaatcctga tttgtagcga gcatgacgcc
     9601 agcatttgta atttgcagat cggtaacctg gcatgattca taaacagcca ctgcaaattt
     9661 ttgctgtcgt gcctggtcta ctaatcgtaa aaattgatcg cggaaatatt cgcccagcag
     9721 aattcgcggt aaaaactgac gatcgtgcaa ggtttctttt ttaacgccat aacgctggag
     9781 atggctggct tcttgctttt gtagccattc gagatacgta caattaatcg gcggtatttc
     9841 aatactggca atatttgcca gcatcatttt tgagttttcc tcatcactgt atggcatccc
     9901 gacaccggcc tcgtcagcct gctcgaagat agaaatagaa agtggagttt gttgctgtag
     9961 aagcgagaat aaggtgtaga tccccgtagg cccggcaccc acaatagcaa tttttttcat
    10021 tgttacttac ctgtacgcat tctttcgaat ttagtgttat cagataagtt tggttgagat
    10081 ttttagaatt tgagaaattt cagacaggga tgaaaagggc gctaaccgcc cttgattaaa
    10141 ttctgtcagg cgagttttgc ctgattgatt tgactcagtg gctcattgac atcgaaaact
    10201 ttggcgacaa cttcctcaac gccgagattt ttcagtcggg cagtgtgttt ttccagatag
    10261 gcaagggcgc ttttttcatc agtgaaaagg tagatcccac cggcttcgtg gttcttttca
    10321 ctttcggtcc ataccttcca cagaaaacca ggttcctgat taatcgactc agcaagcggt
    10381 ttaagctgct cagccattgc gtcgccaaat gggccattga aagcaaaatg aagttgtaac
    10441 aaagttgcca tagcgattct ccttctgata acgcatacga agacagtgta aatcttttaa
    10501 cgtattggat acagagaaga aacagaggga aaaaggagaa gaccagatta agaaaatctg
    10561 gtgcgtccga gtggactcga accaccgacc cccaccatgt caaggtggtg ctctaaccaa
    10621 ctgagctacg gacgcaggat ggtgcgttca attggactcg aaccaacgac ccccaccatg
    10681 tcaaggtggt gctctaacca actgagctat gaacgcaacg ttgtaggtga caacggggac
    10741 gaatattagc ggcagagtgg gaaggtggca agaggcaaaa cgtaattttc tgcgctattt
    10801 cgaccgtttg cagagctttt aagcaaattg gctatatttt gttgatttgc aagggcgatt
    10861 tttattcagg atcgcattta catctgatac aacccaataa ttcgtgtatc agagtttaaa
    10921 cagagttgtt tgccaccatc tcaaatctgt cgtggataaa tcctccgtag cctttacact
    10981 taggtagcaa taaaggcaca cagatgcagc aaaggaataa atcatgggat ctgatgcgaa
    11041 aaacttgatg agcgacggga atgtgcaaat tgttaagacc ggcgaggtca ttggcgcgac
    11101 gcaacttact gaaggcgagt tgattgttga agctggcgga agagccgaaa ataccgtggt
    11161 cacgggggct ggctggttga aagtggcaac cggtgggatc gccaaatgca cacagtacgg
    11221 taacaatggc acgctatcgg tcagcgacgg tgccattgcc acagatattg ttcagtccga
    11281 gggaggcgca attagtctct ctacgctcgc tacggttaat ggccgccatc ccgaaggtga
    11341 attcagcgtt gatcagggtt atgcctgcgg tttgttgctg gaaaatggcg gtaacctgcg
    11401 tgtactggaa ggacatcgcg cggaaaaaat cattctcgat caagagggcg gcctgttggt
    11461 taatgggaca acctcagcgg tcgtggtaga tgaaggtggt gaattgttgg tgtatccagg
    11521 tggggaagcc agcaattgtg agattaatca gggcggcgtt tttatgctgg ccgggaaagc
    11581 cagtgatacg ttgcttgctg gtggcaccat gaataatctc ggtggtgaag actctgacac
    11641 tattgttgag aatggatcca tttatcgtct ggggacggat ggccttcagc tctacagttc
    11701 cggtaagacg caaaacctgt ccgtgaatgt gggtggtcgg gctgaagtgc atgccggtac
    11761 gctggaaaat gcggtaatac aaggtggaac agtgatcctg ttgtcaccca ccagcgcgga
    11821 cgaaaatttt gtcgtagagg aagatcgcgc accggttgaa ctgaccggga gtgttgcatt
    11881 actggacggc gcttcaatga ttattggcta tggcgcagat ctgcaacaat caacgattac
    11941 tgtacagcag ggcggtgtgt tgattctcga cggcagtacg gtaaaaggtg acggtgtcac
    12001 ttttattgtt ggtaacatca atctgaatgg cggaaaactg tggctgatca ctggtgcggc
    12061 aacgcatgtg caactgaaag tgaaacgcct gcgcggagag ggagcgattt gcctgcaaac
    12121 cagtgcgaaa gaaatctcac ctgacttcat caatgtgaaa ggggaagtta ccggggatat
    12181 acacgttgag ataacagatg ccagtcggca aactctgtgc aacgctctga aattacagcc
    12241 agacgaagac gggattggcg caacgctcca gcctgcgtaa aaacacaggc gcaacccgtg
    12301 aaggttgcgc cttgtcttta ttagcgggat gctcgttgca gaatgataac tgacggcaga
    12361 cgttgcagga accgcatacg cagcatcatc ataatggctg ccgacgtcag gccaataata
    12421 aagcctatcc agaagcctgc tggccccata ggttcaacga ccagatcggt cagtgccaga
    12481 atatagccgc ttggcaagcc cagcacccag taagccgtaa aggtgatata gaaaatggaa
    12541 cgcgtatctt tataaccacg caaaatccca ctgccaatca cctggattga gtcagaaatc
    12601 tgatataccg ccgccagcaa catcaaatgc gcagccagcg ttacaacctc gggattgtcg
    12661 ttgtacaaca gggcgatttg ctcccgcagt gaaaccgtga atatggccgt cagggttgcc
    12721 atacagacac ccaccataag cccggtccgc gcagcggttt gcgcatccag cgttgagccc
    12781 tgacccagac gataacctac gcggatagtt accgctgccg ccagcgacat tggaagcaca
    12841 aacatcagtg aactaaagtt cagggcaatc tggtgtcctg cgacatcaac aataccgagc
    12901 ggagacacta acagagccac gacggcaaac agtgtcactt caaagaatag tgccagcgca
    12961 atcggcaaac cgagttgaat cagtcgtttc ataaccgcag gatcgggttt cgcggtgcct
    13021 ttttcgttac gaatatcgcg catggagcgg gcgcgtttaa tgtaagaaac catggcaagg
    13081 aacatgaccc aatacaccgc cgcagtagcc acgccacaac caacgccacc gagctcaggc
    13141 ataccgaaat gaccataaat aaagatatag ttcaccggaa tgttcaccag caggccgata
    13201 aagcccatta ccatacccgg cttggttttt gccagacctt cacattggtt acgggcaacc
    13261 tggaagaaca gatagcccgg cgcgccccac aacaacgcac gcagataacc cacggctttg
    13321 tccgccagag ccggatcgat gttttccatg gagcggataa tgtaacctgc attccacagc
    13381 acaagcataa tgagaacgga aacaaaacct gccagccaga aaccttgtcg cacctgatgc
    13441 gcaatgcgct cacgtcgacc ggaaccattt aattgcgcga taaccggcgt taatgccagc
    13501 agcagtccgt gaccaaagag gatcgccgga agccagatag aagtaccgat agcgaccgcc
    13561 gccatgtcgg tggcactata gccgcccgcc atcacggtat cgacaaaacc catcgcagtt
    13621 tgggcgattt gcgcgagaat caccgggatt gctaatgcta ataacagacg cgcttcactg
    13681 atatacttct gcacgtgaac accttttatt tgtagttata tgagagacta aaaagccgcc
    13741 tgaatgggca gcaaaaagaa gaagcagggg aaattcagtc tattgtagcg aggatttatt
    13801 aattctccag tgaaaaaata gtcattctta ccccttcact ggtaagggcg tttgccacct
    13861 gctattgtgc tgaacagaat gtcttaactg atttcaggag ttgtaagtat gtttacgggg
    13921 attgtacagg gcaccgcaaa actggtgtcg attgacgaga aaccaaattt tcgtacgcat
    13981 gtggtggagt tacccgacca catgctggac ggcctggaaa caggtgcttc cgtggcgcat
    14041 aacggttgct gcctgaccgt gacggaaatt aacggcaacc atgtcagttt tgacctgatg
    14101 aaagaaacgt tacgcattac caatcttggc gatttaaaag tgggggattg ggtaaacgtt
    14161 gagcgtgcgg cgaaattcag tgatgaaatt ggcggacact taatgtcagg tcatattatg
    14221 accaccgctg aagtggcgaa aatattaacc tcggaaaata atcgccagat ctggtttaaa
    14281 gtccaggata gtcagttgat gaaatatatc ctgtacaaag gatttattgg catcgacggt
    14341 attagcctga ccgtcggcga agtcacgcca acgcgttttt gcgtccattt aattccggaa
    14401 actctggaac gcacgactct tgggaagaaa aaactcggcg cacgcgtcaa cattgaaatt
    14461 gatccacaaa ctcaggcagt ggtagatacg gtagaacgtg tgctggcggc acgagaaaat
    14521 gccatgaatc aaccaggtac agaagcctga tctgaaatcg cccccggaga gggggcgata
    14581 gaatagcctt tagcgagcga ctcgaaggcc gttttcaaca ccgcgtgaga acacgacctg
    14641 ccagagctga atatcacggg cgcggaaagc acctgcacag gcattcagat aataggtaaa
    14701 cattcgttta aagcgttcac tgtagttatc cgcaatttct ggccatgcgg cgaggaatcg
    14761 ttcataccac gccatcaacg tagtatcgta atcagcaccg aagttatgcc agtcttccat
    14821 cacgaagtgg ggttcgctgg attgagcaat ctggcgtaca gagggcaggc aaccgttcgg
    14881 aaaaatatat ttattaatcc agggatcaac attcagatcg gtttttttcg aaccgatagt
    14941 atggagcagg aatatacctt ccggtttcaa attacgatcc accaccgcaa aataggtatc
    15001 gtaatttttc ggtccgacgt gctcgaacat ccccacagaa acaatacgat caaactggtc
    15061 gttcaggtca cgataatctt gcagcaaaat ggtgacatcc aggccttcac agcgttcctg
    15121 agccattttt tgctgttcgg cagaaatggt gacgccgacc acgcttacgt catagttaga
    15181 tgccatgtag tgcgccagtc cgccccagcc gcagccaata tccagtacgc gcatccctgg
    15241 ttttaactgc aatttttcac aaatcatttt gagcttcgcc tgctgggcag attccagatt
    15301 atcggcatct ttccagtaag cgcaggaata ttgcatgaag ggatcaagca tgcggctgaa
    15361 caagtcatta cccaaatcgt aatgctcctt gccgactatc caggcacgtt ttttactctg
    15421 cagattgaag agacgagcgc tggcaatacg cagcgtgtct ttgaaatgat gggggagttg
    15481 gttctcgaga cctgcgcgta agactttgct aaaaaacata tccagtcggt cacattccca
    15541 ccagccatcc atataacttt cgcctaaccc caaagagcct tcttgcagaa cgcgtttaaa
    15601 aaaatcgggg tttttcacac gaatatccgc cggggcagaa ccgttaatgg ctataccggc
    15661 acggctaagt aattcgttgg cgatacggta ccagttgtca tccggtacac tgacttcttc
    15721 tatacacgat gaactcatag tttctccatc actggtcggt gatcagaacc ttaaaacagc
    15781 gtagacgctt tttgggcttt gtgagaaatc tcgcagaaaa aaccgcgagt ctataatccg
    15841 acgtagaaca gagggaaagg aggaaccttt gccgaaatga ccatccatag tgagtcggga
    15901 acgatcctgt ccccgtaaaa acataatatt tatcgtagct ttaacaactc gaaattagta
    15961 taggccgcta atttcaatca tacaacaaaa ttttgttagc agcctaagta taagtatatc
    16021 gatatagatc agtgtgattc gctatgagcg acttcggcat tgccatgatt ctggcagcca
    16081 acttcttcac aacgttgcat catgtaaccc agagcgacca gcactactgt tgataacatc
    16141 acgctggtgg tggtgagcaa tggcgtgctg atactgatca gccaggaaac taccagactt
    16201 gcgaggaagc acagacccag ttgtagagtg ttctgcaatg ctgcggcgcg accagttgcg
    16261 tgtgggaagg gacgcagcgc ctgggcgaca acaatcgggt agatcgcgcc attggcaatc
    16321 gccatcacac agaatgggat caggatttcg accagagaca catggctaat gaagcctgca
    16381 gcccaggtcg caatgacgct gactgcaaac agcaccagca accacggtaa taactgcttg
    16441 ccttgccatt tctgcagcgc ggcgcgacag ccataaccac caatcagaaa cgcgatagtt
    16501 tgcgggacat aacttaaacc aataactgcc gggctgtagc ccatttcact aaggatgaac
    16561 ggtgaaccgg tcagccatgc aaaaaaactg gctgaacagg ccgcgtatat cagcacgttg
    16621 ccgcgatagg ttttagaacg tagcaggtcg gtaaaggtca gaccatcctg actattgtta
    16681 cgggccttcg tcgtgggttt gagccagaaa ataggcagaa tcagcaccac ggtaatggca
    16741 aacagggtgg cgaaaatcgc ctgccaggaa aaatggacca gcagccagct tcctaacaga
    16801 ggagccaggg ccggagatag acccaccagc ggcatgatgg tcgcaaaaat tcggttaact
    16861 ttctgtgaag gataataatc cgtcactaac gcttgccaga taaccgccgc ggcgcagaca
    16921 cccacagcct gtacaaaacg caataccagc agcgtagcgg cgttttctac ccacagcatc
    16981 cccagactac ctaacgcaaa aattgtcagg ccgattaata acaccggttt acgaccataa
    17041 cggtcggaga gtggccccca cagaagctgg gctgcggcaa aaccggcaag gaacagacta
    17101 aggctggcac tgacagcaga cgcaggcgtt tgcaggtcgg cctgtatggc ggcgaaagca
    17161 ggcagataca tatcggttgc cagaaaaccg agtacgctca aacccgccag ccagactaaa
    17221 aatcttttcc caggttgcat gtatattctc taaatctctc aattaaacac cgccgcaaag
    17281 tgtagggagt gaaattagtg ttgtgaaacg gtaatatttg atgactggtt tcaaaaattt
    17341 tgcaggcaga ctatgtggtc agaatattca ctcgaagttg ttgatgcggt agcgcgtaat
    17401 ggtagtttta gcgctgcggc acaggagctg catcgcgttc cttctgcggt cagctatacc
    17461 gtgcgtcagc tggaagagtg gctggcggtg ccgctctttg aacgacggca ccgtgatgtg
    17521 gagctgaccg ctgctggagc gtggtttctc aaagaagggc gctctgttgt caaaaaaatg
    17581 cagatcaccc gccagcaatg tcagcagata gcgaacggct ggcgcggtca gttagctatc
    17641 gcagtggata atattgtcag gccagaacgt acacggcaga tgatcgttga tttttatcgc
    17701 cattttgatg atgtcgaact tcttgtcttt caggaagtgt tcaacggtgt ctgggatgcg
    17761 ctttccgacg ggcgcgtgga actggcgatt ggcgcaacac gcgcgattcc ggtaggcggt
    17821 cgttatgcct tccgggatat ggggatgcta agctggagtt gcgttgttgc cagccaccac
    17881 ccgctggcgt tgatggatgg cccattcagc gatgatacgt tgcgcaactg gccgtcgttg
    17941 gtgcgcgaag acacctcgcg aacgctacct aaacgtatta cctggttgct ggataaccaa
    18001 aaaagagttg tcgtgcctga ctgggaatca tcggcaacct gtatctcggc aggattatgc
    18061 atagggatgg tgccaacaca tttcgccaaa ccgtggctta atgaggggaa gtgggtagca
    18121 cttgaattgg aaaacccctt cccggattcg gcatgttgtc tgacatggca gcaaaatgat
    18181 atgtcgcctg cgttaacctg gctgcttgag tatctcggcg atagtgagac gctgaataag
    18241 gagtggctgc gggagccgga agagactccc gcaacgggtg attaacgacg atagtcgcgg
    18301 aacgggccgt cagccacgga gcggcgttca atcaagcgcg gatgcacttc aatggactgc
    18361 ggttcttcac gtttgttgac gatacgatcc aacagcatgt tgaacgctgt ttcacccagc
    18421 gaatcttttg gctggtggat cgtggtcagc gccggcgtaa aatagcgggc gttgcgcacg
    18481 ttatcataac cgatcagcga aacatcctgc gggacgcgca gacccatttc atcagcagca
    18541 caaagtgcgc ccattgccat gatatcgcca ccacagaaga cggcagtagg gcgatgcggc
    18601 tgcgacagta tttgctgcat ggcgcgataa ccagattcgg gttcaaagtc accctgaaca
    18661 atccagcttt ccggcacctt gatcatcgct tcttccatcg ccttcataaa accggcaagg
    18721 cggcctgcgc cggtgttacg ttccagcggg ccggggatga cgccgatttc gcggtgaccg
    18781 cgttcaatca gataacgccc ggccatgtag ccgccttcga acgcgttatc aatgaccgca
    18841 tcggtgaagt cagcttttgc ttcaccccag tccatcacca ccattgggat atggcgatac
    18901 tcttccagca tcgccagcaa cggctctggg tactcagaac acatcaccag cagaccatcg
    18961 acgcgttttt gcgccatcat cgacagataa gcccgctgtt tctcaagatt gttccacgca
    19021 ttacccagaa tcagggtgta acctttctgg aagcaatttt tttcaaccgc ttcaatgatc
    19081 tcggcaaaat aggccgcttc gctgctggtc gccagcaaac cgatagactt ggtgtggtta
    19141 accttcaggc tacgcgccac cgcgctaggg gagtagtgta attctttaat cgctgcccac
    19201 acggcgttgc gcgtttcttc agcgacgaaa cgtgttttgt tgattacgtg tgacacagtt
    19261 gtagtggaaa cgtttgctcg tttcgctaca tcttttattg ttgccattcc atttcactcc
    19321 agaccctaac ttcagctcct gcaaaatcgc aaggtaaacg tttgccttta cacaccttac
    19381 gcacaacaaa agtgttgcgg tacgccggaa aaacgacacg gagcgtcagg aaggggtcaa
    19441 tggccagtac gcaaatagtg taagcgtgga attttggccg atcttgacga aaaggggaag
    19501 tggtaaaaac acttatcatt ataaatgacg ggagattttt tcatcacagt gtgtaaaaat
    19561 gcgtaatatc actatttgtg tggtaataaa aaaggagaaa acttgatgag caccgacctt
    19621 aaattttcac tggtaacaac gattatcgtc ctcggtttga tcgtggccgt gggtttgact
    19681 gccgcgctgc actgatttcc tctcgaggga gcgcgttctc cctcgcatct ccttcctgtt
    19741 tgttagcaaa atggcaaaag tgttttctct ttttgttatt ttcatttttt tgtgattgct
    19801 gtcatgcttt cgccacttta atgaatatcg cgtaatgacg cgaagaggtg gagataaggc
    19861 atgaaaatta actatccgtt gctggcgctg gcgattggcg cgtttggtat cgggacaacg
    19921 gagttctcgc caatgggttt gttgcccgtc attgcgcgcg gtgtggatgt ctcgattccc
    19981 gctgccggaa tgttaatcag tgcctatgca gttggcgtaa tggttggcgc gccgctgatg
    20041 acgcttctac tttctcatcg tgcccgccgc agtgcgttga ttttcctgat ggcaattttc
    20101 acgctcggca acgtactttc cgccatcgcg ccggattata tgaccctgat gctttcacgc
    20161 attttgacca gcctgaatca cggagcattt tttggtttgg gttcagtcgt ggccgcaagc
    20221 gtggtgccaa aacataaaca ggccagcgca gttgccacta tgtttatggg gttaaccctg
    20281 gcaaatatcg gtggcgtgcc ggcggcgacc tggttgggtg aaaccatcgg ctggcggatg
    20341 tcatttctgg caacggcggg gctgggagtg atttcaatgg taagtctgtt cttctcatta
    20401 cctaaaggtg gtgcaggggc acgacctgaa gtgaaaaaag agctggcggt attaatgcgt
    20461 ccgcaggtgc tgtctgcatt gctgacgacg gtactgggag ctggtgcaat gtttactctc
    20521 tacacctata tctctccggt actgcaaagt attacccacg caacaccggt gttcgtcacg
    20581 gcaatgctgg tgctgattgg tgtcggattc tctatcggta actatctcgg cggcaaactg
    20641 gcagatcgtt cagttaacgg cacgttgaaa ggctttttgt tgttgttgat ggtgattatg
    20701 ctggcaatcc cgttcctggc ccgcaatgag ttcggcgcag ctattagcat ggtggtgtgg
    20761 ggcgcagcaa cctttgcggt cgtaccgccg ttacagatgc gcgtgatgcg tgtcgccagt
    20821 gaagcgccag gtctgtcttc atcagtcaat attggtgcct ttaatcttgg aaatgcgctg
    20881 ggagcagctg ctggtggtgc ggtaatttcc gctgggctgg gatacagctt tgtgccggtg
    20941 atgggagcga ttgtcgcggg actggcatta ttgctggtgt ttatgtcagc cagaaaacaa
    21001 cctgaaacag tttgcgttgc taacagctaa ttaaacaaaa acgcagaagg ataaccttct
    21061 gcgttacagt tgctgacaac gtgcgattta tcttgccgaa tgcgctgtta acgcctcatc
    21121 cgcgttggca tggtaagcga agcgcatcag gcaatgctgc atttgccatc agttattatg
    21181 cagcgagatt tttcgctacg aattcccagt tcaccagcgc ccagaagtgc tccagatagc
    21241 caggacgtgc attgcgatag tcgatgtaat aagcgtgttc ccagacatca acggtcagca
    21301 gcggagtcgc atcggtggtc agcggagtac ccgcgttaga ggttgaaacg atagccagtt
    21361 tgccatcgct gtttttcacc agccaggtcc agccagaacc aaagtttttg atcgctgcat
    21421 cagtaaactg cgctttgaaa tcggcaaagc tgccaaaaga tgcggcgata gcttcagcga
    21481 cttttccagt cggttcgcca ccggcgttcg gtgccaggca gttccagtag aaagtatggt
    21541 tccagacctg agctgcgttg ttgaatacgc caccttcaga gctgcgaata atctcttcca
    21601 gtgatttacc ttcaaacgcg gtacctttaa tcaggttgtt caggttagtg acataagtct
    21661 gatgatgctt gccgtagtga tactcgatag tttccgcaga aatgtgcggt gccagagcat
    21721 ctttagcata tggtagtgca ggtaattcga atgacattgc tactctcctt tattattaat
    21781 ttgcatacgt acaatagcct tattgtgcgt atggataggg tagcaaatta aaaggtgaga
    21841 caaaagatga acttaccctg ttaccgtaac aacagggtaa agttttagcg aagtgttttt
    21901 ggggtcatta cccgacgagc gccaacatag tggcgctgcc agtagtcttc actgagagaa
    21961 gtgatttgaa tttcctgacc tgtgcgcggt gactgaataa atttgccgtt gccgacatac
    22021 acgccgacat gatcggctgt gccgcgtccc tgagtacgga aaaagaccag gtcgccgttt
    22081 ttcagttcac tacgttcgat tggcgctgca tcacgcaggt gatacatttc attcgccgta
    22141 cgcggaatac gaattttcac caaatcttta taagcgtaat aaaccaggcc gctgcaatca
    22201 aaaccggtac gcggtgagct gccaccccaa cgatatggct taccaatttg ctgcatcagt
    22261 ttattcattg ccactttcgt agctttttgc accttcgctt tgtgcgcgtc ggcaagagtt
    22321 cctgacgcgg catttttgac tttcacacaa tgcgatttat agcccttacg tttggtacac
    22381 ttttcggtaa agctggctga ggccgtttta ttgacggcgt gtttgctgcg agttttggcg
    22441 tttttagaag cggtaatgga agatttttta acggaagaag cggttttgct tttggtcgta
    22501 cttttactgg cggctttttt cgctgttttc tgggtctttt tggtggttgc cgtgcttttc
    22561 tttttatctg cttttttggt gatatgagtg gtagcagaac tctccctggc ttgctttgaa
    22621 gcatgggcca taggcgttaa aggcagggtg gtaaaaagta aagcacagag cgtgatcgaa
    22681 atacggttta tccgcgccac tgagcgtccc tctgttaagg caggtctatc atgcctgcgt
    22741 ctgatttaca aaacttgatg attctaatgc atagaagaag agaaagttaa tagcttttat
    22801 tgttaaaaaa tacgtttcgt tggcgagcgc aaaaaaatgc agcagtgaaa gctaaaaatg
    22861 gttataaata cgccagtttg ccggcatcag agaagaaacg aatatttgaa atgcaacaaa
    22921 tagagcaaag ggataaaata gcaaaagcgt gatgaaaatg ttattttccg atgccgcact
    22981 gacccgctac aatgtcagat tactgccgta aagaagtttg aggaagcaag acaatgagca
    23041 ccactatcga aaaaatccaa cgccagattg ctgaaaaccc gatcctgctg tacatgaaag
    23101 gttcaccgaa actgccgagc tgcggtttct ctgcccaggc agtccaggcg cttgccgcat
    23161 gtggcgaacg ttttgcctat gttgatattc tgcagaatcc ggacattcgt gcggaactgc
    23221 cgaaatatgc taactggccg accttcccgc aactgtgggt tgacggcgag ctggtcggcg
    23281 gttgtgatat cgtgatcgaa atgtatcagc gtggcgaact gcagcagctg atcaaagaaa
    23341 ctgccgctaa atacaagtct gaagagcccg acgcggaata atccgtcctg caatactgac
    23401 ggatgcgaac gggcatccgt cagtcctttc tctatcccca atccagccct tgtggcgaac
    23461 ttgaaaatcc cgcctcacgc agcagcgtaa acatcggcgt ttgccggacc ggtagatcat
    23521 tcacttctgt tagcgtaaag cgcagccgtg gttcgcgacg cagtgcggta gtcagcgcgt
    23581 ggaaaacctc tggggcgagt aattcctctt tttcctgcca gaccagcatt tttttgccac
    23641 cttgcgccag atagagtaac aatttgccgc cagaaacgac caccagcgca cccgcccgac
    23701 gcgttggaac cagcgttgcc ggatgtgcag gccagggcag aagatttccc cacacattag
    23761 ccggatcgtt ggcagagagc gccactggtg tatagtggcg cgtttgcgtg gcttgtgtcg
    23821 ccagatcgcg caatcggtca atagtcagac gttcagcgaa ttgcgcgcca cccagacctt
    23881 ctacaaaacg acctcgcata attcgcccgg agtcttccat acttcgacaa agcgtttgca
    23941 tcgatggaaa cccgccaggg atattttcgg ctatcaccgc ctgacgactg atgatgccgt
    24001 agcggtcgag catattttcc gccagcgcca gcatcctttc ggtatcgttt agtggctcca
    24061 cctgcaataa cgaccagcgt ccagccagat ttggtgtgtt gtaagatacc agcggcgaga
    24121 cgggttgcgc atagacagga cgtccacgac gagcccggtg actgcggcga gttgaggtgc
    24181 gtgcgttgga actgctgcgg gtgagggcgc gtaacggtgc ccaaatgtcg ctggtgatga
    24241 cgccttgcca gaccagcgcc cataacgcct cttgcagggc agagagatca accgattcgc
    24301 cgattttgtc gcgtatccgt tggctgattt gttgtgcaaa ccaggctcct ccgtcagcca
    24361 gaacagcgac tatcgcttgt tgcagcgccg aacgattcgc ctgatccgct tcggcgggag
    24421 tgaacgattc tgcagcatat tcctgtagat gcagtgccac caggccgtca tcttcaccca
    24481 gttttttttg ccccgaccag ataactgcac cggttgccag taattcatcg agcatttctg
    24541 acgaatagtc gcgtacgcgg gcaggcagga tctggctttc ccagagtgag gcgggtaaac
    24601 cgactccggc aagctgttcg atcacccgca tcacgccatc tacgccctca taaacgcctg
    24661 gcgatgttga ggcaaagagc gccgggctac catcggtggc gggtaatacg ccctgacgtt
    24721 ccagcagcaa tcgcgcatag gtcgtggctg caacgggacg cgtcgcttct ctggcggctt
    24781 gcagcgagcg caaacgcaga cgacgaaata cttcatcgct cacccagatg tcttgttgca
    24841 gattcatcac cagaccctgt tcacgcagtt gctgaagctg ctcttcgaca atggcaatac
    24901 cgagactaaa ctcatgagcc agttgttcag ccgtgaccag agcatgagcc cggagatagc
    24961 gcagaaagag gtcgcgaagc gggtaactga ctctatgtaa ataaatctct ggcaatgact
    25021 ctggtagtcg tacgccgagg gcatcacgca gcctggcggc atcatccata caggcaagac
    25081 gctcctgtcc gctaatcatc gctgggaaga ttcgttttac tgcaagaaga ttttccagat
    25141 acgacgcaac ctcttcactg ctgcctgtat gccgttgcgc caggtcttca acggtcattg
    25201 gccccagttc gcgcagcagg tcgaacaatc cttcttcacc tttcgctctt ctgccaggag
    25261 ccagtcgttg caactcttct tccacctggc gaatgacctg cgggtcgagt aattcccccg
    25321 gatcgacctg tccgagtaga ttgcgcagta actcgctgtc cagcgacagt acggatgccc
    25381 ggcgctctgc cagcggggcg tcgctctggt acataaattc cgcgacatag ccgaacaata
    25441 aacttgtggc gaaaggcgag ggcgtagtgg tcgttacatc ggatatttga atttcgccac
    25501 cgttcaggcg acgcatcaaa cgttccagtg cgggaagatc ataaacatct tgcagacatt
    25561 cgcgtagggt ttcgagaatg accggaaaat ccggatatcc ctgagcgatt tccagcaact
    25621 gactggcgcg cagccgttgt tgccaaagcg gggtgcgatg gcccggagtg cgccccggca
    25681 ttaataatgc ccgcgcggcg cattcgcgaa aacgggcggc gaaaagtgcc gagctgccta
    25741 ccgcctcgcg gacaatttgc agcaactttt ctggttcaaa caaaaaaatc gcggcgtcgg
    25801 gcaatttacc atcagtgtca ggaatgcgcg caacaatgcc gtcatcactg gcgaccacag
    25861 acgcgtcagc gccccatagg gcatgtattc gcccggtaat cgccagcgcc cagggttcat
    25921 gcacccgtct tccataggga gagtgcaaaa taatacgcca gtcgccaatt tcatcacggc
    25981 accgttcgag gaccagatgg cgactccccg gaacgatgcc cgtcgtgttg cgttgctcgt
    26041 caatcaaccc ctgaatattg gcgttcgtat tttcttctgc cagccacggg ggaatagtcc
    26101 cggaaaagaa cgcgccatcc gccagcaaat gaagaaaatc gccgatcatc tcgcctaatt
    26161 cagccggacg tccgttacct tcaccacgcc agaaggggag ccgggcagaa cgacccggag
    26221 caggagtcac aatcacctga tcgcgggtga tttgctggat ccgccatgag gtagcgccga
    26281 gagtgataat gtcgttcacc cgcgactcat ataccatctc ctcatccagt tcacccaccc
    26341 gccgcgaacc ggccttttct tcaccttcgg gtaataacac gctatacatg ccacgatccg
    26401 gaatggtgcc gccgctggta accgccaaca attgagcgcc aggtcgggcg gtcaatatcc
    26461 cggtctccct gttccagacc agtttggggc gaaaagcaga aaaatcgcca gagggatagc
    26521 gcccggaaag catatccagc gtggcgtcaa aaacacgtct tggcagatct ttccacggtg
    26581 cggcacggcg tacgcgggag taccattcgt ctacctgtaa tgcctccatc gccgcggcgg
    26641 caacggtttg ctgcgcaagg acgtcgagtg gattatgcgg cggtgtcagg ttttccagcc
    26701 tgcctgcgaa catacactct acaatgactg cggaatcgac taagtcacgc cgggtacgag
    26761 ggaaaaacag ccctttagat acaccgccaa cctgatgtcc ggcgcgacca atgcgttgta
    26821 acccactggc aacagaaagc ggcgttgcca cctgaatcac cagatccact gcgcccatat
    26881 caatccccag ttcgagactg gaggttgcga ccacgcagcg taactcaccc gatttcagcg
    26941 cctgctcggt gattgctcgt tgttctttag agacggagcc gtggtgtgag cgggcaataa
    27001 aaacgtcgct actttgtaca cggttagagg ttgcgccgga ggtcgactcg aaatgggccg
    27061 catcaacggc gatagacggg gaacgctgta agcgtgcggc gtaaagctca tttaatcgtg
    27121 ccgtcagttt ttccgccagc ccacgtgaat tagtaaagac aatggtcgag cgatggcgca
    27181 acacttcatc aaggataccc gtttcaatat atggccagat ggagccttcc cggccggcat
    27241 ggctgtcttc gccggtgctg ctggcgaccg atgagacatc atccatattg gcgaccggta
    27301 cgacaattcg tatctgcgga tggcgcattg cgggcgggtt gactaccgta accgggcgat
    27361 cgccaccaag aaatgctgcc acatcgctgg ctgagcgcac agtggcagaa aggccaattc
    27421 gctgtgctga ggtgtggagc agcgcatcga gccgctccag acttaacgcc agatgcgcac
    27481 cacgtttact gcccgctacc gcgtggactt catcaataat taccgtttcg acgccgcgta
    27541 gcgtttcgcg cgcgcgggag gtcagcatca gatagagtga ttcgggtgtg gtgatcagaa
    27601 tatccggcgg attacgggtg agtttgctgc gttcctgtgc aggcgtatcg ccagtacgga
    27661 tccctacgcg aagattgact tccgtttcgc cgcgccgccg ccgttcatcg gcaataccct
    27721 tcaacgggag ctgcaagttg cgctgaacgt cggtgcccag agcttttatc ggtgaaatat
    27781 agaggatgcg tgaggtttta cgcttatgcg cctcgcgggt atcttcgccg ccttcgcgga
    27841 agagccgatc gagggcgtag agaaatgctg ccagcgtttt cccggagccg gtcggtgcaa
    27901 tcaccagcgc atgttcgctt cgcgccgcca catgccaggt ttgcgactgg acagcggtcg
    27961 gctgtttaaa ggcgcgaaga aaccagtcgc gggtcgccgg tgaaaacacg tccggcagga
    28021 gcgatgaagg gtctggatta tctgccatta acgctattcc ccgtgaatat catgcaggat
    28081 gcgtggaata gcctcactat agatgcctga atcattgcat gtcatcaggc atcgactcga
    28141 ttacacctct tcggcggcag gtagcggcca gcctcccaga cgtttccagc ggttgacgat
    28201 ttcacaaaac agcacagcag tgcgttcggt gtcgtacagc gcggagtgcg cctgggtgct
    28261 gtcgaagtcc atgccagcgg tctggcaagc ctttgacaat acggtttgtc cgagtgccag
    28321 cccggccagt gcagcggtgt caaaagtggc gaaagggtgg aacgggttac gtttcagtga
    28381 ggcgcgttct gcggcggcca tcataaagct gtgatcaaaa ttggcattgt gcgccaccat
    28441 aatggcgcgg ttacagccgc tcgctttaat acctttacgt acaactttaa aaatttcgtg
    28501 cagcgcctcg tattcgctga ccgcgccgcg atcgggatcg ttcgggtcaa tgccgttgaa
    28561 ggcgagggcc tctggctgca aatttgcgcc gacaaatggt tcgacgtgga aatgtaatgt
    28621 ggtgtccggc atcagccagc cttgttcatc cattttcagg gtgatggcgg caatctcaag
    28681 cagcgcatcg gttttggcgt taaatccggc tgtttcaaca tcgatcacaa caggataaaa
    28741 accacgaaaa cggtcgcaca gaccggtaag ttgagcgtta tcggacatct gattctctta
    28801 atacggataa attgcagcgc gcattatgac aaatattact tgtcgatgca gtaaagatgc
    28861 gggcgcgatg agttcacgcc cggcaggaga ttagttgccc agaccgcgac cggcgtcttt
    28921 ttcttcgatt aactcaattt tgtaaccgtc cggatcttcc acaaacgcga taaccgtagt
    28981 accgcctttt accggacccg cttcacgggt cacgttaccc ccgttttgac ggattttttc
    29041 gcacgcttca gcggcgttat ctacgctaag cgcgatgtga ccataagcag tgccgagttc
    29101 gtatttatcc acgccccagt tgtaggtcag ttcaatcacc gcttcttcgg tttccgggcc
    29161 gtagccaaca aacgccagtg agtatttgta ttccgggttt tcgctggtac gcagcagttt
    29221 catgcccagc actttggtat aaaaatcgat ggagcgttgc aaatcgccaa cgcgcagcat
    29281 ggtatgaaga agacgcatct ttttatcctc aaaatggcta acagattcaa aatgttgttt
    29341 tagtatagcg gcggctttac gccgctcgca atgttggatt acaacgtcgg gtaatcggta
    29401 tagccttccg cgccgccacc gtagaaactt tcggcacgct gtgggttaag ctcagctttg
    29461 cgctgcaagc gggcgaccag atccgggttc gcaatccagt cacgaccaaa tgccaccgca
    29521 tcaattaacc ctttgccgat cagcgtttca gctttttcta ctgtgtatgc acctgcgccg
    29581 ataatcggac cgtggaaacg ggcgcgtact ttttcgcgga acgcatcagt atacggttca
    29641 ccccccgccc aatctggttc tgacatatgc agataagcaa tgccgcgttt acccagttgt
    29701 tcaatcagat acagtgcatc ggcttcttca ttcgggccgt tatctgtgtt ctggaaagta
    29761 ccgattggcg aaacgcgaat gccaatgcga tcggcacccc attcttcaat cccggcatcg
    29821 accacttcca gtaccaaacg tgcgcgattt tccacgctgc cgccgtactg atcggtacga
    29881 tggtttgaag aaggagaaag gaactgatgc agcaaataac cgtgagcaga gtggagctct
    29941 accagatcaa aaccggcttc acgcgcgtta gcaatggcct gacggaaatc attgacgata
    30001 cctggaatct cttccagttc aagcgcacgc ggcatggatg tttcaacacg gatcgcctga
    30061 ccattttcat cgcgcagaga agtacgtgtt cccgcgctaa gtgctgaagg cgctaccggt
    30121 gcctgaccgc caggttgcag gctggcgtga gaaatgcgtc cggtgtgcca cagctgcacg
    30181 gccatatgac cattttcagc atgaacgcca gcggtgattt ttttccatgc ggcaatttgc
    30241 tccggactat ggatgccagg cgcacctgca tatccttttg cctgggcaga aatttgcgtg
    30301 gcttcactaa taatcaaacc ggcactggca cgttggcgat agtattccgc catcaacggg
    30361 gtaggaatgt cacccggttc aatactgcgc agacgcgtca gcggtgccat aaaaatacgg
    30421 tttgccgccg tgatcgcgcc cactttcagt ggggaataca gtttttcaga tgacataaag
    30481 gctcccggtc gtctggtgat aaaaaatgcc tgctaaacgg caggcgtcgc aataatgttt
    30541 tttacatggg ccagcgcgtt ttccagtggc acgaaactgc gcgaaatttt ggcctgcaga
    30601 ttcgcgccaa gccacagtgc gtaaagcact tgtgcctgtt gtagcggttc gccacaaaag
    30661 gttaaacaat ggttctcacg gccattttcc agcgcctgag agagcagggc gatcacgccg
    30721 cgagcacctt tatccatcgc gctgcgcata tcttctgaca gatcgcacac ttcggcagag
    30781 agttttactg tcaggcaacc actgatggtt ccatgttggc aaaactggtt cagtgtttgc
    30841 tggtaataag ccagtatgcg gtcgcggtag ttaccttcgc cggattgcag caactcagtc
    30901 agtcgctggt gatatgcggc gtaatgacgc tcaagcatgg caacgccaaa cgcttcttta
    30961 gagcgaaagt agtgatagaa ggaccctttc ggcacttcag cggtttttag taattcgctt
    31021 agccccatcc cggtgaatcc acgttgcagg caaagttgct cgcccgtcgc caggagatgt
    31081 tcgcgagtat catgttcggt gtgtttgttc atgcgttgga gtgtagtaga ccagtcggtc
    31141 tagtgcaagc aaagttgcta ctttggacat taacagcttc aataatggta ataatcttat
    31201 ctgaggaggg cgaagtggcg gagcaattag agttctttcc cgtccagagc ccgtgccggg
    31261 gaatttgcca gtctgacgaa cgcgggtttt gtcgcggctg ttttcgtagt cgtgatgagc
    31321 gttttaactg gaataaaatg agcgatggcg aaaaacaaga ggtgctgcgc ctttgtcggc
    31381 agcgattaat gcgcaaatta cgcgcaaata agccagcgtc atcagacgaa ccggagcaac
    31441 catcactctt ttgaacctaa tcttgcgtat actcctctca tatctgataa gaggaagtga
    31501 ttatggttca gcgtattact attgcgccgc aaggcccgga gttttcccgt tttgtgatgg
    31561 gctactggcg attgatggac tggaatatgt ccgcccgcca gctggtcagt tttattgaag
    31621 agcatctgga tctcggcgtg accaccgtgg accatgctga tatttatggt ggctatcagt
    31681 gcgaagcggc gtttggcgag gcactgaaac tggcacctca cctgcgtgaa cggatggaaa
    31741 tcgtcagtaa atgcggtatc gcgacgaccg cgcgtgaaga aaacgtcatt ggtcattaca
    31801 tcactgaccg cgatcacatc attaagagcg ccgaacagtc gctaattaat ctcgcgaccg
    31861 atcatctgga tctgctgtta atccaccgac cagacccgtt aatggatgcc gatgaagtgg
    31921 cggacgcgtt caaacatctg catcagagcg gcaaagtgcg tcattttggc gtatcgaact
    31981 ttacgcctgc gcaatttgcc ctgttgcaat cacgtctgcc gtttaccctt gccactaatc
    32041 aggtggaaat atccccggtg catcagccgt tactgctgga tggcacgctc gaccaactac
    32101 aacaactgcg tgttcgtccg atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg
    32161 atgattattt ccagcagctg cgtgatgaac tggctgtggt ggcagaggag ttaaacgcgg
    32221 gctcgattga acaggtggtt tacgcctggg tattacgttt accatcgcag ccgctgccaa
    32281 ttatcggttc aggtaaaatt gagcgcgtac gggcagctgt cgaagcagaa acactgaaaa
    32341 tgacccgtca acaatggttt cgtatccgta aagcggcact ggggtacgac gtaccgtaag
    32401 cacttttagg aatagccgcc gttcaaaaat gtgtcactgg tttacactta ttcaggaatg
    32461 cacaatgaac ggaggtccta tgaaacgttt tagtctggct attctggcgc tggttgttgc
    32521 aaccggcgca caagctgcca gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt
    32581 agggcagtca attggtagcg tcaccattac tgaaaccgat aaaggtctgg agttttcgcc
    32641 cgatctgaaa gcattacccc ccggtgaaca tggcttccat attcatgcca aaggaagctg
    32701 ccagccagcc accaaagatg gcaaagccag cgccgcggaa tccgcaggcg ggcatcttga
    32761 tccacaaaat accggtaaac atgaagggcc agaaggtgcc gggcatttag gcgatctgcc
    32821 tgcactggtc gtcaataatg acggcaaagc taccgatgcc gtcatcgcgc ctcgtctgaa
    32881 atcactggat gaaatcaaag acaaagcgct gatggtccac gttggcggcg ataatatgtc
    32941 cgatcaacct aaaccgctgg gcggtggcgg tgaatgctat gcctgtggtg taattaagta
    33001 attaagaggc cagcgtacct tgcggtggtg cttgctcaag ttgcgaaagc gagcagtaca
    33061 gccgccagac aattgccgcc agctcacggg cggcaggttg atgatgacgg gcaaggctgt
    33121 cgcaaatccg ctgtaattct tcaagtgtgg ccgccagtga tttttgctga acgccacgct
    33181 cactcatcac tccccgcaac agtgaaatac agttatcccg tactcgcgat aacggatcgg
    33241 aacgcgattc ccagtcgcgc aattgccaga caacatgaga acagttcagc agcactacac
    33301 cccagcgtaa taaccaacgg cgagccagcg catcctggct gttactcaac tgactgacgt
    33361 gatgataagt gagcgattca aattcgcttt cactcagtgt tggatggcgg cttagctgat
    33421 cgacaaaatc ccggcgcagc gcgcgaatat ggcggcggct tttacgagca tccgatcctg
    33481 gacgcagaat ggcgaacgct agccaagcca gcgcaacgcc aacgattttt gccagattat
    33541 cgttaagaaa atcagcaaaa tcatacaccg ggggattagt gacagcgata aacgaaccca
    33601 taaaaacaat cagttgcccc cacagtgcgg caaatttggg catctgcaat ttaagaagct
    33661 gcattgtcgc cagcagtgga aagagaaaca gtaaaaattg ccacagatcg ctaatctgga
    33721 ccatcaggcc aaatttgacc acaaagctga atagcgaaag taacaccagc gtgcgcatca
    33781 gcagtgacaa cgacttaaac ggtgctgcga cggcggagta gagtacgcag ctaattgctg
    33841 ccagcgttaa tgcattggca ccggcatccc attgcgatgc aatactccat gcgccaatca
    33901 tcatcaacgt acaaaatgta cgcagcccgc tccacatagc ttcggcgttg tcggtatgac
    33961 gagccagagc ggatgtccgt gcgagtctgg catgatcatc tacaccgctt tgcagacgat
    34021 gtaattcctg actactttgc agataaaggc ggcaaaaata acgtagtcgc tgccagaagg
    34081 cgacgtgccg gtagtcggcg acgttggtcg ggcgtagcgg ggcgataata cgtgcgacgg
    34141 tgtaagcatc tgtttgcgaa ctggcgagcg ccgtcagcaa ttgttcgaga atttctcgcg
    34201 tggcacctgg cggtgagggc cagttgagca acatacgtcg caggctggag atgacactgg
    34261 tcatacgtaa ttgctggtgg agtagcgcat taaggcgcgc gttttgctgg cgaaaacgat
    34321 agtggctcca gaaagcctgg atacgcagca aattcatggt cagtatctgc ccaatcaccc
    34381 cctcatgtgc tgcacgaatg gcatcgtttg tttcaggctg ccagagtaaa ctggcatgtt
    34441 ccagtaatcg ggcgtgcatg tttttcaatg cggttaaaag ggctgtagca tcggaactgc
    34501 taggcaggat catcatcatc atgccgccgc acaaaatacc gactatcacc tcgcaaacgc
    34561 gcgcctgagc gatatcccac agctggctgg cctcagtaat attaaccatc ggaaaggcga
    34621 tgatggcagc cgtgtagccc gccagttgaa atgcatacgc gacgttattc gtgaagtgcg
    34681 cacaggccca ggtacaaaag ccaagccacg ccgacatgct caatagaaaa aaccacggct
    34741 cattgagcgt atgcccggca agaagcagtg ccgcaatggc tcccagtaaa ctgccagcga
    34801 tgcgtccgag gcttttgctg atgacaccgc caacggtggg aaagctaacc actgcagccg
    34861 aggtcatcgc ccaatagggt tcatccagat tcaaataata ggcaaccgtc agcgccagac
    34921 acatggcaat ggtattgcgt aacgcataac gccattgcgc cagcgtggcc ttgaaccagg
    34981 gcaaattgcg caaggaccat gacgatgcgt tcattattga cctacggcaa tgctacaggt
    35041 ggtgcccgac accagcgtga catcgcctgg aactttatcc agcgcaaagc ggacgggaac
    35101 gcgttgggcg agacgaaccc aggggacgtt aggtttcaca tccggaataa ggcttgagtc
    35161 actttcaacg ctttgatcat aaatcgcccg accgatactg gaaacgtgac cccgtaacgt
    35221 tttgttatca ctatagaggg taatttgtgc tggtgcacct tcgcggatat ggcgcaactt
    35281 ggtttcttca aaataaccaa tgacgtaaaa cgagtggctg tcgacaaggg caaacagcgg
    35341 tttcccggtg tcagcgtaat cgccgatgcg ggtagtgagg ttagtcaccc aaccactcac
    35401 cggggcgcga atttccgttt gcgccagttg ccattgtgtc tgtttcagcg tggcctgtgc
    35461 ggcattaaca ctggcctgca tcgctttaac attgaggtta gcggtatcga gttcttcggc
    35521 agaaataaaa ttttgtgaga gatgacgacg acgattcgct tcgttattgg cttttgcgag
    35581 gtcagattgc gctttcgcca gctgagcctg tgcgttcagc tcggcgatct gaaagggcgt
    35641 tttatcgatc gtgagcagaa gatcccccgc gttaaccagt tggttgtctt ttatattcag
    35701 ctcaacaata cgtcccgaca cctgtggggt gatagaaacc ttttctgcgc gtattttccc
    35761 gtcgcgtgtc cacggagatt gcatgtaata attccacagc caccatccgg caagaaccgc
    35821 aactatcgcc acaatgattg ttgagaaata cttaattgtt ttaattgaca tagttaccac
    35881 gcaatcagta ttgccagagc aaggcaaacg caaatcgcaa acagcgataa atccattaac
    35941 aagggatgcc agatgtcacc ggcgtagatc cagccacgaa gcaggcggtg tacgacaagc
    36001 cagatgacga atccaaacgc gaaggctttg aaaaacggag gaaaatagac ggacgcaccg
    36061 aacaccagat cttgtaaggg caatcctgtt gcattgagca taaacttcac aaagtagatt
    36121 cctttacgac cgaatatatt gcgttagatt aataaatatt ctttaagtgc gaaaaattta
    36181 cgcgcaattt ctgaaaaata tgtctgacgg taaccaaatg cagcaataca tttgttttag
    36241 caatacaatt gctgcacact attctaaaag ccgcataata tcttagcaag ctaattataa
    36301 ggagatgaaa ttggaatcgc cactaggttc tgatctggca cggttggtgc gcatatggcg
    36361 tgctctgata gaccatcgcc tgaaaccgct ggagttaaca caaacccatt gggttacgtt
    36421 acacaatatc catcagttac ctccagacca gtcgcaaatt caactggcaa aagcgattgg
    36481 catcgagcag ccatcactgg tccgtactct ggaccaactg gaagaaaaag ggttaatttc
    36541 gcgtcaaact tgtgccagcg atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga
    36601 gccactgatc agcgaaatgg aagctgttat taacaaaacc cgcgcggaaa tattacatgg
    36661 catctccgca gaggaactgg agcaactgat tacgctcatc gcaaaacttg agcataatat
    36721 cattgagtta caggccaaag ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca
    36781 cgcaaactta tttagcgcgg agaaacggtc acctgactgc cattgctggc cagtacgaca
    36841 cgttggcccg gagagaaacg agtgttgcct tgtttctgta ccaccatgat ggtattacca
    36901 tcgtctttac gaatttccag ctcgacaccc tgcgttttgt tcattgcact ctgtacgccc
    36961 tgaccagcta cgccacctgc aacagcgcct gctgcagtag ccagagaacg cccggttccg
    37021 ccaccaacag tattccccag gaaaccacca agaacagcac cgccaattgc accgataacg
    37081 ttggaatcat caccgccctg aatctgtacc ggacgtacgt taacgatggt gccatagctg
    37141 acattctgta cttgtttcgc ttcagaaacg gtataaacat cccctgacag ggtgtcgtta
    37201 ttaacacaac cgacaagaga cagacctacc attgaaacaa ccaatacgcg tttaatcatt
    37261 gaaaaatctc ctgttcacca tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg
    37321 gcatatgtga atccatggtt acatgttcta ccaaatatga gcaaatcata atgaaagtag
    37381 gatcaaccaa ttataaacaa aacattcatg atgattattg atttgaagat aatgttttac
    37441 taccaacggt aaaaatcggt cattccgcat ggtattcacg aaaagtttat gtttgagtgg
    37501 tgcgcaaacg tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt attggcgtta
    37561 tgtcaggcac cagccttgat ggtgttgatg ttgtgttggc gacaatagat gaacaccggg
    37621 tcgcacagct ggcaagcttg agctggccga tcccggtgtc tctgaaacag gctgtactgg
    37681 atatttgcca gggccagcag cttacacttt cgcagtttgg acagcttgat actcaactcg
    37741 ggcgactttt tgctgatgcg gtcaatgcct tgcttaagga acaaaacctg caggcgagag
    37801 atatagttgc gatcggctgt cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac
    37861 cacacacttt acagattggc gataacaatc aaattgtggc acgcaccgga attacggttg
    37921 tcggtgattt tcgccgtcgc gatattgcct tgggaggaca aggcgcaccg ctggtacctg
    37981 cgttccatca tgccttgctg gctcactcaa ccgagcgacg aatggtgctc aatattggcg
    38041 gcatcgccaa tctgtcactg ctcattcctg ggcagccggt tggcggctac gataccggtc
    38101 ctggtaacat gctgatggat gcctggatct ggcgtcaggc cggtaaacct tacgataaag
    38161 atgccgagtg ggcacgggcg ggtaaagtta ttctcccact gctgcaaaat atgctcagcg
    38221 acccatattt ctcgcaacct gcaccgaaaa gcaccggacg cgaatacttt aactacggct
    38281 ggctggagcg ccatttacgc cattttccgg gtgttgatcc ccgtgatgtt caggccacac
    38341 tggcagaact caccgccgtg accatttctg aacaagtttt gttgagcggt ggctgcgaac
    38401 gattgatggt atgtggtgga ggtagtcgta atccgctact catggcgcgt ctggcggcat
    38461 tactgccagg cacagaagtc atcaccaccg atgccgttgg cattagtggc gatgacatgg
    38521 aagcattggc tttcgcctgg cttgcctggc ggacgctggc gggattacca ggaaatctgc
    38581 cttccgtcac tggcgcaagc caggagacgg tactgggggc tattttccct gctaacccgt
    38641 gatatcagag ttaactgaat ttttcttacc gcgtctgccg ttacacttgg aacgttaggg
    38701 agggcgtatt gccctccaga ccaggaaagt cttcgggaaa tgtctatgaa aaaactgtta
    38761 ctgatctgtt tgccggtgct tctcaccggc tgtagcgcgt tcaatcaact ggttgagcac
    38821 atgcaaaccg ctacgctgga ataccagtgt gatgaaaaac cgttgacggt caaactgaat
    38881 aatccgcgcc aggaagtcag ttttgtttac gataatcaac tactgcatct caaacagggc
    38941 atttcagcgt ctggtgcacg ttacactgac ggaatctatg ttttctggtc gaaaggcgat
    39001 gaagcgactg tctataaacg cgaccgcatc gtcttgaata actgtcagtt acaaaatcca
    39061 cagcgttgag atttttccag gggcggcgca caatagcgtc acccactgac aatccgtaaa
    39121 gaaaaccatg tctgataacg acgaattgca gcaaatcgcg catctgcgcc gtgaatacac
    39181 caaaggcggg ttacgccgtc gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg
    39241 gctctctcag gcgtgtgaag ccaaactggc agaccctacc gcgatggtgg tcgctaccgt
    39301 ggatgaacat gctcagcctt atcaacgcat cgttttactc aaacattacg acgaaaaagg
    39361 catggtgttt tacaccaacc tcggtagccg taaagcacat caaattgaaa ataatccgcg
    39421 cgttagcctg ctgttcccgt ggcataccct tgaacgccag gtgatggtga tcggtaaagc
    39481 agaacgactt tcgactctcg aagtgatgaa atattttcat agccgcccgc gtgatagcca
    39541 gattggtgca tgggtttcga agcagtccag tcgcatttct gcccgcggta tccttgaaag
    39601 taaattcctg gagctgaagc agaagtttca acagggcgaa gtgccattgc cgagcttttg
    39661 gggcggtttt cgcgtcagcc ttgaacagat tgagttctgg cagggcggtg agcatcgcct
    39721 gcatgaccgc tttttgtacc agcgagaaaa tgatgcgtgg aagattgatc gtcttgcacc
    39781 ctgaaaagat gcaaaaatct tgctttaatc gctggtactc ctgattctgg cactttattc
    39841 tatgtctctt tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac
    39901 atggagattt tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta
    39961 gcccaggtga cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc
    40021 tattgcggct tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta
    40081 tgcctgaaac gcttccagca ggcgggccac aagccggttg cgctggtagg cggcgcgacg
    40141 ggtctgattg gcgacccgag cttcaaagct gccgagcgta agctgaacac cgaagaaact
    40201 gttcaggagt gggtggacaa aatccgtaag caggttgccc cgttcctcga tttcgactgt
    40261 ggagaaaact ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg
    40321 accttcctgc gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg
    40381 gttaagcagc gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac
    40441 ctgttgcagg gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt
    40501 ggtggttctg accagtgggg taacatcact tctggtatcg acctgacccg tcgtctgcat
    40561 cagaatcagg tgtttggcct gaccgttccg ctgatcacta aagcagatgg caccaaattt
    40621 ggtaaaactg aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc
    40681 taccagttct ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc
    40741 tttatgagca ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca
    40801 ccgcgcgccc agtatgtact ggcggagcag gtgactcgtc tggttcacgg tgaagaaggt
    40861 ttacaggcgg caaaacgtat taccgaatgc ctgttcagcg gttctttgag tgcgctgagt
    40921 gaagcggact tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc
    40981 gcagacctga tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt
    41041 aaaactatcg cctccaatgc catcaccatt aacggtgaaa aacagtccga tcctgaatac
    41101 ttctttaaag aagaagatcg tctgtttggt cgttttacct tactgcgtcg cggcaaaaag
    41161 aattactgtc tgatttgctg gaaataatgc attaagtgga aagggggagt gagaaatcac
    41221 tccccctggt ttttatacag ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt
    41281 tgtttatggt catgcgggta acagtgcggc agagtttcct atgcgccgcc tgggcgcgaa
    41341 cgtctggccg ctgaacaccg ttcaattttc taatcacacc caatacggca aatggactgg
    41401 ctgcgtgatg ccgcccagcc atttaaccga aattgtgcaa ggcattgccg ccattgataa
    41461 attacacacc tgtgatgccg tgttaagtgg ctatctggga tcggcggagc agggtgaaca
    41521 tatcctcggt atcgtccgtc aggtgaaagc cgcgaatccg caggcgaaat atttttgcga
    41581 tccggtaatg ggtcatccgg aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca
    41641 tgtgcggcac ggtttgcctg ccagcgatat cattgcgccg aatctggttg agctggaaat
    41701 actctgtgag catgcggtaa ataacgtcga agaagcggtt ctggcagcgc gcgaactcat
    41761 tgcgcaaggg ccacaaattg tgttggttaa acacctggcg cgagctggct acagccgtga
    41821 ccgttttgaa atgctgctgg tcaccgccga tgaagcctgg catatcagcc gtccgctggt
    41881 ggattttggt atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt tactgctggt
    41941 gaaactgctt cagggggcaa cgctgcagga ggcgctggaa catgtgaccg ctgcagtcta
    42001 cgaaatcatg gtgaccacca aagcaatgca ggaatatgag ctgcaagtgg tggctgctca
    42061 ggatcgtatt gccaaaccag aacattactt cagcgcaaca aagctctgaa atatctcatt
    42121 tcggcccaca acgacctgtg ggccgatgga tattacttta agccttccgc tgacagcgcg
    42181 tcttgtactt ccggacgttc agccatacgt tgcataaatg ctgcaatgtg ctctaaccct
    42241 tccagattca gtttcaccgc gtatgcccag cgcagaaccg taaacagata ggcatcagca
    42301 attgtaaatc tttgcccgca gatccagtgc tcatccttca gtgcctcgtt cacatattgc
    42361 agcttcttct ccagctgcgc gcgaactgtc ggtttgtact cttccggtgt atcagggcga
    42421 aacagaggtg tgaaaccttt atgcagctcg gtggcgatgt aattcagcca ttcgatggtt
    42481 ttatagcggg aaatactgtt taccggtgcc agcaactggc ggtcggggac gctgtcggca
    42541 agatactgca taatcgctac gccttccgtc agcaaagtac cgtcatccag cagcaatgca
    42601 ggcacctgcc ccttagggtt aacggcaaag taatcgtcac cgttttcgag acgttttttc
    42661 attaaatcca cactgacgag ggtaaaatcc tttccgctct cacgcagggt gatatgggaa
    42721 gcgagagagc aggcacccgg tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg
    42781 tttaaagtat ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc
    42841 ttgtaaaaga gtttccctga attttacgct acagcagctt tcgccgcttt gtctgcagcg
    42901 tcatcctgcg tcatacggtg cagtttaggc gcggtcagca gcatcagtac tgcaataatg
    42961 gcagtagcga caccaatctg caagaatacg cgaccataaa cttccagtga catcagcggg
    43021 tcggtaacgt tatccggcac agccatcata cccgcaacat aaccaccaat caggtttgca
    43081 ccggcagtgg tcaggaacca gctacccata atgaagccca tcagacgctg cggaacgagt
    43141 tgtgcaacca ttgccagacc cagaccagag atcatcagtt ccccgatgct ctgcaggcca
    43201 tagcttgcga ccagccagct tacagacacg ataccagcgt cagacgcgaa tttcgcaccc
    43261 agcggcagaa tcaggaacgc accagaacac atcaccatgc cgattgcaaa cttggttggc
    43321 atcggcaggg tatcgcccat cttgttatag atagcggcca gaatcggact accgatgatg
    43381 atccagaacg ggttcagtgc ctgatactgc tcaggttcta cggccagacc cagaatggag
    43441 tgctcaacgt tacgaatcgc aaagaagttc agtgacgttg gcatctggct gtacagcacg
    43501 aagaagataa tggcttcgag catcaggatg aaggcaacga tcattttacg acgcgcagca
    43561 cctttcatcg cgaaggcttc tttaccgaag ataaccacga taccgaaggc aacaacgccc
    43621 agcgccatac gcgcaacttc ctgattgtgc agcagccagg tggcgatagc gatcagtgcc
    43681 acaacaccaa taatggtcag cagcaggtta cggtagttga taggctcgaa gtctggtttt
    43741 gaaccgtact gtttaaccca gcgttggcag aaggcgaagt taacgatagt gatcagcagg
    43801 cctacaacgc tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag ccacggcgta
    43861 gcaatcatag agaagaaaga gccgatgttg acggacatgt agtacatggt gaatgcaccg
    43921 tccagacgcg ggtcgttttt ctcatagcat gtagaaagca gagaagacgg gttagcttta
    43981 aacaggccgt taccgaccgc aatagccgcc atacccatat aaacgatacc ggcgtcgtga
    44041 ccagaccagg caaccagagc ataaccaatc gccagcacaa tagcgccgag cataattacg
    44101 cgtttagtac ccagtacctt gtcacctaac cagccgccga tagcgaccag accataaacc
    44161 agggcactaa aggaagagaa aagggtgatt gaatccgctt cagacatacc cagttgttta
    44221 accaggtaaa cagccataat tccttgtagg ccgtaataac caaaacgttc ccataactca
    44281 atcgagaaga tgagatagaa cgccttcggt tgtttgaaag cgttcaaact gacgctttca
    44341 gttggttttt ggtttgcagt ggacactttt acctcttttt ttacatccca tattaacggg
    44401 ggtgctttct cgcgttatgt ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa
    44461 cggcaggtaa tgttcactat cttgcacttt cagacaagtc ctttgtaata atctgttaca
    44521 tataactgag gcggggaatt caaccattcc gggaaatgtt atccagcgtt aaatttttca
    44581 aaaaacgtaa cggcagattt tttcactgca ctgagagggg gtgatggctt aacggcgata
    44641 tgttctgcta tttagcgttt ttactagtga gatagtccag tttctgaaaa atagccagtg
    44701 taatgttttg taggtcaata tttgttgcgt gtgtaaattt aaatgatttt aacctgattc
    44761 tt
//
LOCUS       DAZFRN010000020        44695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFRN010000020 DAZFRN010000000
VERSION     DAZFRN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.020"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            30..113
                     /locus_tag="ACHKUB_001089"
     CDS             30..113
                     /locus_tag="ACHKUB_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654136.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            complement(187..1389)
                     /gene="ubiI"
                     /locus_tag="ACHKUB_001090"
                     /gene_synonym="visC"
     CDS             complement(187..1389)
                     /gene="ubiI"
                     /locus_tag="ACHKUB_001090"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO6654137.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1412..2590)
                     /gene="ubiH"
                     /locus_tag="ACHKUB_001091"
                     /gene_synonym="visB"
     CDS             complement(1412..2590)
                     /gene="ubiH"
                     /locus_tag="ACHKUB_001091"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO6654138.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2587..3912)
                     /gene="pepP"
                     /locus_tag="ACHKUB_001092"
     CDS             complement(2587..3912)
                     /gene="pepP"
                     /locus_tag="ACHKUB_001092"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO6654139.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3938..4516)
                     /gene="ygfB"
                     /locus_tag="ACHKUB_001093"
     CDS             complement(3938..4516)
                     /gene="ygfB"
                     /locus_tag="ACHKUB_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO6654140.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4684..5013
                     /gene="zapA"
                     /locus_tag="ACHKUB_001094"
     CDS             4684..5013
                     /gene="zapA"
                     /locus_tag="ACHKUB_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6654141.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            5055..5238
                     /gene="ssrS"
                     /locus_tag="ACHKUB_001095"
     ncRNA           5055..5238
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKUB_001095"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5259..5861
                     /gene="fau"
                     /locus_tag="ACHKUB_001096"
     CDS             5259..5861
                     /gene="fau"
                     /locus_tag="ACHKUB_001096"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO6654142.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5962..6021)
                     /locus_tag="ACHKUB_001097"
     CDS             complement(5962..6021)
                     /locus_tag="ACHKUB_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6654143.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6354..6413)
                     /gene="ibsC"
                     /locus_tag="ACHKUB_001098"
     CDS             complement(6354..6413)
                     /gene="ibsC"
                     /locus_tag="ACHKUB_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO6654144.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6642..7874)
                     /gene="serA"
                     /locus_tag="ACHKUB_001099"
     CDS             complement(6642..7874)
                     /gene="serA"
                     /locus_tag="ACHKUB_001099"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO6654145.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8130..8789)
                     /gene="rpiA"
                     /locus_tag="ACHKUB_001100"
     CDS             complement(8130..8789)
                     /gene="rpiA"
                     /locus_tag="ACHKUB_001100"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO6654146.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(8845..9030)
                     /locus_tag="ACHKUB_001101"
     CDS             complement(8845..9030)
                     /locus_tag="ACHKUB_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654147.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            9172..10065
                     /gene="argP"
                     /locus_tag="ACHKUB_001102"
     CDS             9172..10065
                     /gene="argP"
                     /locus_tag="ACHKUB_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO6654148.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10269..12413
                     /gene="scpA"
                     /locus_tag="ACHKUB_001103"
     CDS             10269..12413
                     /gene="scpA"
                     /locus_tag="ACHKUB_001103"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO6654149.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRGVLNLISQHHD"
     gene            12406..13401
                     /gene="meaB"
                     /locus_tag="ACHKUB_001104"
     CDS             12406..13401
                     /gene="meaB"
                     /locus_tag="ACHKUB_001104"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO6654150.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13412..14197
                     /gene="scpB"
                     /locus_tag="ACHKUB_001105"
     CDS             13412..14197
                     /gene="scpB"
                     /locus_tag="ACHKUB_001105"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO6654151.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14221..15699
                     /gene="scpC"
                     /locus_tag="ACHKUB_001106"
     CDS             14221..15699
                     /gene="scpC"
                     /locus_tag="ACHKUB_001106"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO6654152.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRALYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNNYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15696..16592)
                     /gene="ygfI"
                     /locus_tag="ACHKUB_001107"
     CDS             complement(15696..16592)
                     /gene="ygfI"
                     /locus_tag="ACHKUB_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6654153.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16759..17499)
                     /gene="yggE"
                     /locus_tag="ACHKUB_001108"
     CDS             complement(16759..17499)
                     /gene="yggE"
                     /locus_tag="ACHKUB_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO6654154.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(17592..18227)
                     /gene="argO"
                     /locus_tag="ACHKUB_001109"
     CDS             complement(17592..18227)
                     /gene="argO"
                     /locus_tag="ACHKUB_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO6654155.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(18366..19226)
                     /gene="mscS"
                     /locus_tag="ACHKUB_001110"
     CDS             complement(18366..19226)
                     /gene="mscS"
                     /locus_tag="ACHKUB_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO6654156.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            19422..19520
                     /locus_tag="ACHKUB_001111"
                     /pseudo
     CDS             19422..19520
                     /locus_tag="ACHKUB_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19584..20663)
                     /gene="fbaA"
                     /locus_tag="ACHKUB_001112"
     CDS             complement(19584..20663)
                     /gene="fbaA"
                     /locus_tag="ACHKUB_001112"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO6654157.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(20878..22041)
                     /gene="pgk"
                     /locus_tag="ACHKUB_001113"
     CDS             complement(20878..22041)
                     /gene="pgk"
                     /locus_tag="ACHKUB_001113"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO6654158.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(22091..23110)
                     /gene="epd"
                     /locus_tag="ACHKUB_001114"
     CDS             complement(22091..23110)
                     /gene="epd"
                     /locus_tag="ACHKUB_001114"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO6654159.1"
                     /translation="MIVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            23482..23913
                     /locus_tag="ACHKUB_001115"
     CDS             23482..23913
                     /locus_tag="ACHKUB_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO6654160.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            23936..24514
                     /locus_tag="ACHKUB_001116"
     CDS             23936..24514
                     /locus_tag="ACHKUB_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO6654161.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            24515..25222
                     /locus_tag="ACHKUB_001117"
     CDS             24515..25222
                     /locus_tag="ACHKUB_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO6654162.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            25210..25887
                     /locus_tag="ACHKUB_001118"
     CDS             25210..25887
                     /locus_tag="ACHKUB_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6654163.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLKN
                     GALQPLC"
     gene            25881..26558
                     /locus_tag="ACHKUB_001119"
     CDS             25881..26558
                     /locus_tag="ACHKUB_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6654164.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(26530..27243)
                     /locus_tag="ACHKUB_001120"
     CDS             complement(26530..27243)
                     /locus_tag="ACHKUB_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO6654165.1"
                     /translation="MNIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQAVSSQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVVKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(27240..27749)
                     /gene="fumE"
                     /locus_tag="ACHKUB_001121"
     CDS             complement(27240..27749)
                     /gene="fumE"
                     /locus_tag="ACHKUB_001121"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO6654166.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(27771..28736)
                     /gene="glpX"
                     /locus_tag="ACHKUB_001122"
     CDS             complement(27771..28736)
                     /gene="glpX"
                     /locus_tag="ACHKUB_001122"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6654167.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(28733..30010)
                     /gene="yggP"
                     /locus_tag="ACHKUB_001123"
     CDS             complement(28733..30010)
                     /gene="yggP"
                     /locus_tag="ACHKUB_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6654168.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(30025..31413)
                     /gene="cmtA"
                     /locus_tag="ACHKUB_001124"
     CDS             complement(30025..31413)
                     /gene="cmtA"
                     /locus_tag="ACHKUB_001124"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO6654169.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GSMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(31441..31884)
                     /gene="cmtB"
                     /locus_tag="ACHKUB_001125"
     CDS             complement(31441..31884)
                     /gene="cmtB"
                     /locus_tag="ACHKUB_001125"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO6654170.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(32198..34189)
                     /gene="tkt"
                     /locus_tag="ACHKUB_001126"
     CDS             complement(32198..34189)
                     /gene="tkt"
                     /locus_tag="ACHKUB_001126"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6654171.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            34467..35225
                     /gene="loiP"
                     /locus_tag="ACHKUB_001127"
                     /gene_synonym="yggG"
     CDS             34467..35225
                     /gene="loiP"
                     /locus_tag="ACHKUB_001127"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO6654172.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(35281..36024)
                     /locus_tag="ACHKUB_001128"
     CDS             complement(35281..36024)
                     /locus_tag="ACHKUB_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO6654173.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(36011..37120)
                     /gene="dgaE"
                     /locus_tag="ACHKUB_001129"
     CDS             complement(36011..37120)
                     /gene="dgaE"
                     /locus_tag="ACHKUB_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO6654174.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGYIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(37124..37984)
                     /locus_tag="ACHKUB_001130"
     CDS             complement(37124..37984)
                     /locus_tag="ACHKUB_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO6654175.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(37981..38730)
                     /locus_tag="ACHKUB_001131"
     CDS             complement(37981..38730)
                     /locus_tag="ACHKUB_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO6654176.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(38756..39241)
                     /locus_tag="ACHKUB_001132"
     CDS             complement(38756..39241)
                     /locus_tag="ACHKUB_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO6654177.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(39252..39680)
                     /locus_tag="ACHKUB_001133"
     CDS             complement(39252..39680)
                     /locus_tag="ACHKUB_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6654178.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(39799..42597)
                     /gene="dagR"
                     /locus_tag="ACHKUB_001134"
     CDS             complement(39799..42597)
                     /gene="dagR"
                     /locus_tag="ACHKUB_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO6654179.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(42856..43776)
                     /gene="speB"
                     /locus_tag="ACHKUB_001135"
     CDS             complement(42856..43776)
                     /gene="speB"
                     /locus_tag="ACHKUB_001135"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO6654180.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(43912..44643)
                     /locus_tag="ACHKUB_001136"
     CDS             complement(43912..44643)
                     /locus_tag="ACHKUB_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654181.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT        10877 a        12144 c        11136 g        10538 t
ORIGIN      
        1 tgaataaggc aatattaaga gctgaaaaaa tgtcaaaaaa cactaaatca aaaattcgtg
       61 acattagaaa atataatgcg aaaacggagg tgaaattagt ttatttcaaa tgaggaaaat
      121 ctcccggcga aaaaaccggg agatgaaagt gtgatgggta tcaaataaac agcagaagag
      181 aaatttttaa cgcagccatt caggcaaatc gtttaatccc attgcctggc ggataagttg
      241 tggtttaacg ccaggaagcg tgtcggccag tttcaaacca atatcacgca gcagtttttt
      301 cgccggattg gtaccggaaa acagatcgcg gaatccctgc ataccagcca gcatcagcgc
      361 cgcactgtgc ttgcggctac gctcataccg gcgcaggtaa atgtactgcc cgatgtcttt
      421 cccctgacga tgcaaccgtt tcagttcagc aatcagctct gcagcatcca taaagccgag
      481 atttactccc tgccccgcca gcgggtgaat ggtatgcgcg gcgtcgccca ccagcaccag
      541 acggtgcgcg gcaaactggc gcgcataacg ccccgtcagt gggaacacct gacgcgcgct
      601 ctcaaccttg cataagccca ggcgattatc aaaagcgata tttaacgcgc gattaaattc
      661 atcttcactt gcctgctgca tccgctgcgc ttcctctggc gacagtgacc agacaattga
      721 gcaaagatgc ggatcgctaa gcggtaaaaa ggccagaatg ccttcgccat ggaaaacctg
      781 ccgcgccacc gcatcatgcg gttcttccgt gcgaatggtc gctaccagcg cgtgatgctg
      841 ataatcccag aaagtcagcg gaatatctgc tttgttgcgc aaccaggaat tagcaccgtc
      901 cgcgccaatc accagacgcg ccgttaacat gctgccatct ttcagcgtca ggaaagtttc
      961 attttctccc caggcgacct gctgtaattc tgcgggggcc aacagagtga tatctgacga
     1021 ctgctgcgct ttgttccaca gcgcgtagtg aatcactgag ttttcaacga tatgcccaag
     1081 atggctatag cccatgcttt gatcgtcaaa cgaaatgtga ccaaaactgt ctttgtccca
     1141 cacttccata ccgtgataac aactggccct acgagagaga atgtcctgcc agacgccaag
     1201 acgggtgagt aatttttcgc tggcggcatt gatagccgaa acgcgcagtt gtggtggtgc
     1261 atccgccgcc agaggttccg gtacgcgctg ctccagtacg gcaacgcgta agccgctccc
     1321 ctgtaagccg caggcaaccg ccagccccac catgccgcca ccaacaatgg ctacatcaac
     1381 actttgcatt gtttattcct taaaaccgcc ttcaacgcgc cacccaaccg agggtgcgct
     1441 gcgccagcac atcgcgtgcc ggggtgaata attccatcgt catcagtccg atgttgcgcc
     1501 cgacaaccag cggtgcccaa cggttggcaa aaagatgtac caggctgtcc gtgacgccga
     1561 tggttgcttc gcgatcgctc tgtcgacgct gttgatagcg gcacaatatg ccgtaatcac
     1621 cgatgtcttc tccgcgctca tgcgcctgag ttagggtttc cgcaaggctc atgacatctc
     1681 gcataccgag gttaaaccct tgcccggcaa tcgggtgcag agtttgcgcc gcattgccca
     1741 ccagcacggt acgatgggta atagctctgg cggcgcgggt taacgccagc ggataagcac
     1801 tgcgtttacc ggcgtgggta atttttccaa gtcgccagcc aaaggccgac tggagttcac
     1861 ggcaaaactt ctcgtcgctc cacgacagca cctcttcgcg ccgttccagt ggatgacacc
     1921 agaccagcga acagcgtccg tcagacatcg gcaacatcgc cagcgggcca tgttgcgtga
     1981 agcgttcaaa agcgcggcct tcgtgcgcaa cggaagtcgc aacgttggca atcacggcca
     2041 gttgttcgta aggctcctgc tgccagtcaa cgccgcacgc ggtggctaac gctgaatggg
     2101 tgccatcagc tgctaccagc acgcggcccg tcagcgtctc gccactctcc agcgtcactt
     2161 caacgtgact ctgagtacgg gcaacgttag ccacgcgatc agggcaatgc agcgttacgc
     2221 caggtgcttt acgcagcaat gcaaacagcc gttgcccgac attgtgcaat tcgacaacct
     2281 gtcccagcgc cgccagttgg taatcttctg cggcgagggt gacaaatcca gcgtgaccac
     2341 gatcgctgac atgcacggta gtgatggcgg ttgcgtaatc cgccagagat tgccagacgc
     2401 cgatgcgcgc cagttgctga caggtccccg cagccagcgc aatagctcgt ccatcaaagc
     2461 ccggatgagc atgtgactct ggcgcggtcg cttcaatcaa atgtaccggc aacgccccgt
     2521 gacttaaccg ggaaatagcc agcgccagcg tcgcgcccgc catgccgcca ccgacgatga
     2581 ttacgctcat tgctttctcg cagctgccat caacgcttcg atttcttccg gctttttcac
     2641 cacgctggcg gtgaggtttt cgttgccggt ttcggtaatc acaatgtcgt cttcaatacg
     2701 aatgccgata ccgcgatatt gttctggcac atccgcatcc ggcgcgatat acagaccagg
     2761 ctctacggtc agtaccatcc ccggttccag aatgcgcgag cgatcctgac cataaacgcc
     2821 aacgtcatgg acatctagcc ctaaccagtg gctaaggcca tgcataaaga aaggacgatg
     2881 ggcgttctga gcgatcagtt catcaacatc acctttcaga atgccgagtt ttaccaggcc
     2941 gctaaccatg atgcgcacca cttcaccagt gacttccaga atggaagttc ccggacgata
     3001 caggcgcaag ctggtttcga gagactccag cacaatgtcg tagatttcac gctgggcctg
     3061 agtgaatttg ccgttgaccg ggaaggtgcg ggtaatatcg ccagcgtaac ctttgtattc
     3121 gcaacccgcg tcaatcaaca ccaggtcgcc gtcgcgcatt tcacactcgt tttcggtgta
     3181 gtgcagaatg cagccgtttt caccgctgcc gacaatggtg ttataggacg gatagcgcgc
     3241 accgtggcgg ttaaattcgt ggtgaatttc gccttccaga tggtactcga acattcccgg
     3301 acggcatttt tccatcgccc gggtatgtgc cagggcggtg atttctcccg cgcggcggag
     3361 tacggcaatc tcttctggcg atttgaacag gcgcatttca tgaacaacag gacgccagtc
     3421 gatcatcgtt gccggtgcgg tgagattttg ccgcgaacct ttacgcagtt tttccagcgc
     3481 actgttcacg atttcatcag catatgcata ttcgccctgg gcatggtaaa ccacatccag
     3541 gccgttaagt agttgataaa gttgctgatt gatttcgctg aatgccagtg cgcggtcaac
     3601 gcccagtttc tctggcgcgg catcctggcc taagcgacgg ccaaaccaga tctccgccgt
     3661 caggtcgcga acgcggttaa acagaacgct gtggttatga gtgtcatcgc ttttaatcag
     3721 caccagcacc gcttccggtt cgttaaagcc ggtgaagtac cagaagtcac tgttctgacg
     3781 ataggggtat tcgctgtcgg cgctacgtgt tacttctggt gcagcaaaaa tcagcgcggc
     3841 gctgccgggt tgcatttgct ccaccagggc ctgacggcga cgctgaaact cttgccggga
     3901 tatctcactc ataacactct ccttacgttt tttgttttta gtgtagagtc ggtttttgta
     3961 cttctggcgc ggtcggttgc ggatgagtaa aggtgtcgtg gcataacagc gcggcaacac
     4021 gaacgtattc gatgatctct tcaagcgaca tttcaagctc ttcctgatct tcgtcttcgt
     4081 cgtaacccag ttgcgcaatg ttacgcagat cgtcgatagc ttcaccggtt tcgccggtca
     4141 ctttatccag cttcggttgc gtaacgccaa gaccaagcag gaagtgattg acccaacctg
     4201 ccaatgcatc agcccgatcg aaaacgctga catcatcgcc atcaggcaga taaagctgaa
     4261 aaaggaagcc gtcatcctgc agggcatcgc tggtggcaga gtgcatttta cgcagtgcct
     4321 gtgccagctc atgaccgaaa gccatgcctt cgttcgtcag gtcgtgaagt agcggtagcc
     4381 atgagctgtc atcgttaccg ccacatatca tcccactgat taaaccatgc atctcagcag
     4441 gggtcagacc cgtcccttgt tggttcagat actggttcat ttcgttgtaa ccaggcattt
     4501 cgttctgtat agacataagc attcgtcgtc aaagggagga atattcatga tatgctacca
     4561 ctttgggccc tggtggacca gaaaagggct tgtctcttct catcagggta gctatagtgt
     4621 cgccccttcg cagaccatgg gtctaaagac gaaggcagcg cagtcaatca gcaggaaggt
     4681 ggcatgtctg cacaacccgt cgatatccaa atttttggcc gttcactgcg tgtgaactgc
     4741 ccgcctgacc aaagggatgc gttgaatcag gcagcggacg atctgaacca acggttgcaa
     4801 gatctgaaag aacgcactag agtcacaaat actgaacagt tggtcttcat tgccgcattg
     4861 aatatcagct atgagttagc gcaagaaaaa gcgaagactc gtgactacgc ggcaagtatg
     4921 gaacagcgta ttcggatgct gcagcagacc atagaacaag cgttacttga acaaggtcgc
     4981 atcaccgaaa aaactaacca aaactttgaa tgacactttt cggtttactg tggtagagta
     5041 accgtgaaga caaaatttct ctgagatgtt cgcaagcggg ccagtcccct gagccgatat
     5101 ttcataccac aagaatgtgg cgctccgcgg ttggtgagca tgctcggtcc gtccaagaag
     5161 ccttaaaact gcgacgacac attcaccttg aaccaagggt tcaagggtta cagcctgcgg
     5221 cggcatctcg gagattccct tcttatctgg caccagccat gacgcaacta ccagaactcc
     5281 cactgacatt atcccgacaa gaaatccgca aaatgattcg gcaacgtcgt cgtgcgttaa
     5341 cgccggaaca acagcaggaa atgggtcaac aagccgctac ccggatgatg acttatcccc
     5401 cggtggtgat ggcgcatacg gtcgctgtat tcctctcttt tgatggtgaa ctcgacaccc
     5461 agccgctaat agaacaactc tggcgcgccg gtaagcgcgt atatcttcca gttttgcatc
     5521 cctttagtgc cggtaatttg ctgttcctga attaccatcc gcaaagcgaa ctggtgatga
     5581 acaggttgaa gatccatgag ccaaaactgg atgtgcgtga cgtgctaccc ctttcccgat
     5641 tagacgtgct gatcacaccg ctggtcgcct ttgatgagta cggtcagcgc ctgggaatgg
     5701 gcggtggttt ttatgatcgg accttacaaa actggcagca ctataaaacg caaccggtgg
     5761 gttatgcgca tgattgtcag ttggtggaaa aactccccgt tgaagagtgg gatatccctc
     5821 ttcctgcggt ggttacaccg tcgaaagtct gggagtggta agggcgatac acctgcatcg
     5881 ccctgattga catcgttgat tctttaacct aatttagtga gtaagggtaa gggaggattg
     5941 ctcctcccct gtgactgact gttaataagc tgggaaactt atgagtaaca gtacaatcag
     6001 tatgatggca agtcgcatca taacccttct ccttcaagcc ctcgcttcgg tgagggcttt
     6061 accgttacag ccccatgctg cccagctatc gtaagctctc tttaaataac cacaacgcat
     6121 tgatctacct ttaaatcttt tcttcgagcg tgctcgaaaa accccatgca aaacagagat
     6181 aatttttggg aaaaaggatc tattacggca gtaaacgtgg gcgatgaagt ggctatcaaa
     6241 aagttcggtt cagggcgatg caccccgcat cgccctgatt gacatcgttg attctttgac
     6301 ctaatttagt gagtaagggt aagggaggat gactcctccc ctgagactga ctgttaataa
     6361 gctccgaaac ttatcagtaa cagtacaatc agtatgatga caagtcgcat cataaccctt
     6421 ctcctttaag ccctcgcttc ggtgagggct ttaccgttac agccccatgc tgtcccgcca
     6481 tcgtaaatcc ccattaaata aacacaacgc attgatctga ctttgattta ttttctggag
     6541 cagactcgca aagtagaatg cgcaacgcgg caacggtgtg gagaagggat aaaaaaacgg
     6601 gcaagtcagt gacctgcccg ttaattttca gagaagggga attagtacag cagacgggcg
     6661 cgaatggtac ccggaatagc tttcattgcc tgcagcgctt tttcggcaac gtcttcgtca
     6721 gcttcaatat caataactac ataacccatc tgggcggaag tttgtagata ttgcgcggcg
     6781 atgttgacgc cctgctcggc gaagattttg ttcagcgcag ttagcacgcc cggacggttt
     6841 tcgtggatgt gcatcagacg acgaccacca tgcagtggca gcgagacttc cgggaagttc
     6901 accgcagaga gcgttgagcc attgtcagaa tacttgatca atttacccgc aacttccagg
     6961 ccgatattct cctgcgcttc ctgagtcgaa ccgccaatgt gtggcgtcag aaggacgttg
     7021 tcgaattcac acagtggaga ggtaaatgga tcgctattgg tcgccggttc cgtcgggaat
     7081 acgtcgattg ccgcccccgc cagatgtttg ctcgccagcg catcacacag cgccggaata
     7141 tccaccacag taccgcgcga ggcattaatc agcagcgagc cgggcttcat tagcgaaatc
     7201 tctttcgcgc ccatcatatt tttggtggac ggattctctg gtacatgcag actcaccaca
     7261 tcgctcatat tcagcaggtc agaaagatgc tgtacctgag tggcgttgcc cagcggcagt
     7321 ttgttttcaa tatcataaaa gtaaacatac attcccagcg attcagccag aatgcccaat
     7381 tgcgtaccaa tatgaccgta gccgatgata cccagctttt tgccgcgcgc ttcaaaagaa
     7441 cccgccgcca gtttgttcca cacgccacgg tgcgctttag cattggcttc cggcacgccg
     7501 cgcaatagca gcagcagttc gccaatcacc agctccgcaa cagagcgcgt atttgagaac
     7561 ggtgcgttaa ataccgggat cccgcgcttt gccgccgcat ccagatcaac ctggtttgtt
     7621 ccgatacaga aacagccaat agcgaccagt ttttctgcgg cgttgatcac gtcttcagtc
     7681 agatgggtac gggatcgcag gccgatgaag tgggcatcgc ggatggattc ttttaattgt
     7741 tcatcatcca gcgcgccttt gtgaaattcg atgttggtgt aaccagctgc acgaaggctt
     7801 tccaacgcct tttggtgcac gccttctacc agcagaaact taatcttgtc tttctccagc
     7861 gatacctttg ccatttaccc aatcctgtct tttgaaatgt tgtgtgcgga tttgcatccg
     7921 cctttcaaca tatcaaaaaa taatattgcg gcaatatgaa cgtttgcgtc gcgatgttga
     7981 ggaaatatca cgcaaaggta attcttggat gaatatgctg gtttagagga tttatttaga
     8041 gcaatcgaca attgcctggt aaaagcgtga cacatgtcac caaatttaat gaagagaatt
     8101 tttttaacgg gggaggttcc cccgtcagat catttcacaa tggttttgac accgtcaggt
     8161 gtgccaatca gcgcaacgtc cgcgccacgg ttagcaaaca agccaacagt caccacgcca
     8221 ggaatcgcat ttatggcgtt ttccatcgct atcgggtcaa ggatttccat gccgtggacg
     8281 tcgaggatca cgttgccatt atcggtcacc acgccctgac ggtattccgg acgaccgccc
     8341 agtttcacca gctgacgcgc cactgcacta cgtgccatcg ggataacttc tactggcagc
     8401 gggaatttac ccagaatatc aacctgcttg gaagcgtctg caatacagat aaatttttct
     8461 gcaaccgaag caatgatttt ttcacgggtc agcgccgcgc cgccgccttt gatcatttgc
     8521 atgtggccgt tgatttcatc tgcgccatca acgtagatgc caaggctgtc gacttcgttg
     8581 agatcaaaaa cgtgaatgcc gaggcttttc agtttttcag tggaagcatc tgaactggaa
     8641 acggcccctt caatctggcc tttcattgta ccgagcgcgt caataaagtg tgcggcggtg
     8701 gaacctgtac ctacaccaac aatggtgccg ggctgaacat actgaagtgc cgcccatcct
     8761 actgcttttt tcaattcatc ctgcgtcatg atcgtttcgc ctgtggtatg aaatttcaca
     8821 cgcattatat acaaaaaaag cgattcagac cccgttggca agccgcgtgg ttaactcatc
     8881 cataaaatat cgcgcaatgg cgggcatccc ctttcgcccc gcaaataaag catacaacgg
     8941 tctgggtatg ccgctccacg gtgcgaacag aggcaataac gccacgccat tcaacacaac
     9001 cagggcgcga actcgctgag cgaaatgcat tagcgcaaat ccctgatctt ttaagctgcg
     9061 cgtttaacca cgaatacaaa tgttacccgc cgacggcttc ggtatatgca acctgacaca
     9121 aaattgtgtc atagtgcagg aaaaagcatt taccaggagc agacaacagc aatgaaacgc
     9181 ccggactaca gaacattaca ggcactggat gcggtgatac gtgaacgagg atttgagcgc
     9241 gcggcacaaa agctgtgcat tacacaatca gccgtctcac agcgcattaa gcaactggaa
     9301 aatatgttcg ggcagccgct gttggtgcgt accgtaccgc cgcgcccgac ggaacaaggg
     9361 caaaaactgc tggcactgct gcgccaggtg gagttgctgg aagaagagtg gctgggcgat
     9421 gaacaaaccg gttcgactcc gctgctgctt tcactggcgg tcaacgccga cagtctggcg
     9481 acgtggttgc ttcctgcact ggctcctgtg ttggctgatt cgcctatccg cctcaacttg
     9541 caggtagaag atgaaacccg cactcaggaa cgtctgcgcc gcggcgaagt ggtcggcgcg
     9601 gtgagtattc aacatcaggc gctgccgagc tgtcttgtcg ataaacttgg tgcgctcgac
     9661 tatctgttcg tcagctcaaa accctttgcc gaaaaatatt tccctaacgg cgtaacgcgt
     9721 tcggcattac tgaaagcgcc agtggtcgcg tttgaccatc ttgacgatat gcaccaggcc
     9781 tttttgcagc aaaacttcga tctgcctcca ggcagcgtgc cctgccatat cgttaattct
     9841 tcagaagcgt tcgtacaact tgctcgccag ggcaccacct gctgtatgat cccgcacctg
     9901 caaatcgaga aagaactggc cagcggtgaa ctgattgact taacgccggg gctatttcaa
     9961 cgccggatgc tctactggca ccgctttgct cctgaaagcc gcatgatgcg taaagtcact
    10021 gatgcgttgc tcgattatgg tcacaaagtc cttcgtcagg attaatccat caaataatgc
    10081 ctgatagcac atatcaggcg ttatcctcac ttctttttgt attccttgaa tcacatcaca
    10141 aaatagacaa atctcaggcg gcaaaaaacg acgtctgaat gcattttttt tgctggcgac
    10201 aaacccacgt aaaaagctca ccgtaagcgc aaataccctc attttgattg cgttttacgg
    10261 agcaaataat gtctaacgag caggagtggc aacagcttgc caacaaggaa ttgagccgtc
    10321 gggagaaaac tgtcgactcg ctggttcagc aaaccgcgga agggatcgcc atcaagccgc
    10381 tgtataccga agccgatctc gataatctgg aggtgacagg tacccttcct ggtttgccgc
    10441 cctacgttcg tggcccgcgt gccactatgt ataccgccca accgtggacc atccgtcagt
    10501 atgctggttt ttcaacagca aaagagtcca acgcttttta tcgccgtaac ctggccgccg
    10561 ggcaaaaagg tctttccgtt gcgtttgacc ttgccaccca ccgtggctac gactccgata
    10621 acccacgcgt ggcgggtgac gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata
    10681 tgaaagtcct gttcgaccag atcccgctgg ataaaatgtc ggtttcgatg accatgaatg
    10741 gcgcagtgct accagtactg gcgttttata tcgtcgccgc agaagagcaa ggtgttacac
    10801 ctgataaact gaccggcacc attcagaacg atatcctcaa agagtacctc tgccgcaaca
    10861 cctatattta cccgccaaaa ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt
    10921 ccggcaacat gccgcgattt aataccatca gtatcagcgg ttaccacatg ggtgaagcgg
    10981 gtgccaactg cgtgcagcag gtagcattta cgctcgctga tgggattgag tacatcaaag
    11041 cagcaatctc tgccggactg aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg
    11101 gtatcggcat ggatctgttt atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga
    11161 gcgaagcggt cagtggattt ggcgcacagg atccgaaatc actggcgctg cgtacccact
    11221 gccagacctc aggctggagc ctgactgaac aggatccgta taacaacgtt atccgcacca
    11281 ccattgaagc gctggctgcg acgctgggcg gtactcagtc actgcatacc aacgcctttg
    11341 acgaagcgct tggtttgcct accgatttct cagcacgcat tgcccgcaac acccagatca
    11401 tcatccagga agaatcagaa ctctgccgca ccgtcgatcc actggccgga tcctattaca
    11461 ttgagtcgct gaccgatcaa atcgtcaaac aagccagagc tattatccaa cagatcgacg
    11521 aagccggtgg catggcgaaa gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg
    11581 cctcagcgcg cgaacagtcg ctgatcgacc agggcaagcg tgtcatcgtt ggtgtcaaca
    11641 agtacaaact ggatcacgaa gacgaaaccg atgtacttga gatcgacaac gtgatggtgc
    11701 gtaacgagca aattgcttcg ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa
    11761 ccgccgcgtt gaacgccctg actcacgccg cacagcataa cgaaaacctg ctggctgccg
    11821 ctgttaatgc cgctcgcgtt cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg
    11881 ctttcgaccg ttatctggtg ccaagccagt gtgttaccgg cgtgattgcg caaagctatc
    11941 atcagtctga gaaatcggcc tccgagttcg atgccattgt tgcgcaaacg gagcagttcc
    12001 ttgccgacaa tggtcgtcgc ccgcgcattc tgatcgctaa gatgggccag gatggacacg
    12061 atcgcggcgc gaaagtgatc gccagcgcct attccgatct cggtttcgac gtagatttaa
    12121 gcccgatgtt ctctacacct gaagagatcg cccgcctggc cgtagaaaac gacgttcacg
    12181 tagtgggcgc atcctcactg gctgccggtc ataaaacgct gatcccggaa ctggtcgaag
    12241 cgctgaaaaa atggggacgc gaagatatct gcgtagtcgc cggtggcgtc attccaccgc
    12301 aggattacgc cttcctgcaa gagcgcggcg tggcggcgat ttatggtcca ggtacaccta
    12361 tgctcgacag cgtgcgcggc gtactgaatc tgataagcca gcatcatgat taatgaagcc
    12421 acgctggcag aaagtattcg ccgcttacgt cagggtgagc gtgccacact cgcccaggcc
    12481 atgacgctgg tggaaagccg tcacccgcgt catcaggcac taagtacgca gctgcttgat
    12541 gccattatgc cgtactgcgg taacgccctg cgactgggcg ttaccggcac acccggcgcg
    12601 gggaaaagta cctttcttga agcctttggc atgttgttga ttcgtgaggg attaaaggtc
    12661 gcggttattg cggtcgatcc cagcagcccg gtcactggcg gtagcattct cggggataaa
    12721 acccgcatga atgacctggc gcgtgccgaa gcggcgttta ttcgcccggt accatcctcc
    12781 ggtcatctgg gcggtgccag tcagcgagcg cgggaattaa tgctgttatg cgaagcggcg
    12841 ggttatgacg tagtgattgt cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc
    12901 cgcatggtgg actgttttat ctcgttgcaa attgccggtg gcggcgatga tctgcagggc
    12961 attaaaaaag ggctgatgga agtggctgat ctgctcgtta tcaacaaaga cgatggcgat
    13021 aaccatacca atgtcgccat tgcccggcat atgtacgaga gtgccctgca tattctgcga
    13081 cgtaaatacg acgaatggca gccacgggtt ctgacttgta gcgcactgga aaaacgtgga
    13141 atcgatgaga tctggcacgc catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt
    13201 ttacaacaag tgcggcaaca acaatcggtg gaatggctgc gtaagcagac cgaagaagaa
    13261 gtactgaatc acctgttcgc gaatgaagat ttcgatcgct attaccgcca gacgctttta
    13321 gcggtcaaaa acaatacgct ctcaccgcgc accggcctgc ggcagctcag tgaatttatc
    13381 cagacgcaat attttgatta aaggaatttt tatgtcttat cagtatgtta acgttgtcac
    13441 tatcaacaaa gtggcggtca ttgagtttaa ctatggccgc aaacttaatg ccttaagtaa
    13501 agtctttatt gatgatctta tgcaggcatt aagcgatctc aaccggccgg aaattcgctg
    13561 tatcattttg cgcgcgccga gtggatccaa agtcttctcc gcaggccacg atattcacga
    13621 actgccgtct ggcggtcgcg atccgctctc ctatgatgat ccattgcgtc aaatcacccg
    13681 catgatccaa aaattcccga aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg
    13741 cgcatttgaa atgatcatga gttccgatct gattatcgcc gccagtacct caaccttctc
    13801 aatgacgcct gtaaacctcg gcgtcccgta taacctggtc ggcattcaca acctgacccg
    13861 cgacgcgggc ttccacattg tcaaagaact gatttttacc gcttcgccaa tcaccgccca
    13921 gcgcgcgttg gctgtcggca tcctcaacca tgttgtggaa gtggaagaac tggaagattt
    13981 caccttacaa atggcgcacc acatctctga gaaagcgccg ttagccattg ctgttatcaa
    14041 agaagagctg cgtgtactgg gcgaagcgca caccatgaac tccgatgaat ttgaacgtat
    14101 tcaggggatg cgccgcgcgg tttatgacag cgaagattac caggaaggga tgaacgcttt
    14161 cctcgaaaaa cgtaaaccta atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa
    14221 atggaaactc agtggacaag gatgaccgcc aatgaagcgg cagaaattat ccagcataac
    14281 gacatggtgg catttagcgg ctttaccccg gcgggttcgc cgaaagccct acccaccgcg
    14341 attgcccgca gagctaacga acagcatgag gccaaaaagc cgtatcaaat tcgccttctg
    14401 acgggtgcgt caatcagcgc cgccgctgac gatgtacttt ctgacgccga tgctgtttcc
    14461 tggcgtgcgc tatatcaaac atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg
    14521 agtttcgttg acctgcattt gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc
    14581 gacattgatg ttgccgtcat tgaagcatcg gcactggcac cggatggtcg agtctggtta
    14641 accagcggga tcggtaatgc gccgacctgg ctgctgcgag cgaagaaagt gatcattgaa
    14701 ctcaataact atcacgatcc gcgcgttgca gaactggcgg atattgtgat ccctggcgcg
    14761 ccaccgcggc gcaatagcgt gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat
    14821 gtgcaaatcg atccgaaaaa gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt
    14881 aatatgctgg ataagcaaaa tcccatgtgc cagcagattg ccgataacgt ggtcacgttc
    14941 ttattgcagg aaatggcgca tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc
    15001 gtgggcaata tcaataatgc ggtaatggcg cgtctggggg aaaacccgga aattcctccg
    15061 tttatgatgt attcggaagt gctacaggaa tcggtggtgc atttactgga aaccggcaaa
    15121 atcagcgggg ccagcgcctc cagcctgaca atctcggccg attccctgcg caagatttac
    15181 gacaatatgg attactttgc cagccgcatt gtgttgcgtc cgcaggagat ttccaataac
    15241 ccggaaatca tccgtcgtct gggcgtcatc gctctgaacg tcggcctgga gtttgatatt
    15301 tacgggcatg ccaactcaac gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc
    15361 agcggtgatt ttgaacgcaa cgcgtatctg tcgatcttta tggctccgtc gattgctaaa
    15421 gaaggcaaga tctcaaccgt cgtgccaatg tgcagccatg ttgatcacag cgaacacagc
    15481 gtcaaagtga tcatcaccga acaagggatc gccgatctgc gcggtctttc cccgcttcaa
    15541 cgcgcccgca ctatcattga taattgtgca catcctatgt atcgggatta tctgcatcgc
    15601 tatctggaaa atgcgcccgg cggacatatt caccacgatc ttagccacgt cttcgactta
    15661 caccgtaatt taattgcaac cggctcgatg ctgggttaat ggtttgtccc gtcacgatct
    15721 tcagcaatat gctgaagcat cgtggcggta taacgatgat ttctgattga ataaagatat
    15781 tcctgcatat acatcggcct gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc
    15841 agtcgccagg caaaatgcgg gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt
    15901 ttggccataa aactgtaggg acgataaata atggttggat tgatcccaca aggtcgcata
    15961 ttcgcatcaa tcattgcttc gaagttggcc cggttctgaa agcgcatttg tagccagggc
    16021 agctcctgaa gcagctcttg ctcttgacgc tcttcataac ggcgagaaac gagaaagcct
    16081 aatcgcagcg gtggtagttc actgatagtc aggttttcca gttcctgaac tcgtgcagat
    16141 acatgctgtg gggagataat aaaatctaac tggcggtcaa aaagattatc gataacgccg
    16201 ttttcgctga actcaactgg ctgggcggtc acgccttcat acttatctcc gagactaatc
    16261 aactgatcga aaataattgt cggataagta ttatctatgc caatcacaat ttctcgtgaa
    16321 cgacgtcctg agttatggat ttcattatca atcgcagaaa gccgttgata aaccgggaac
    16381 agtttttgat acagttcctg tccggcttta ttgaggctga tattattatc tttgcgggta
    16441 aatagcgtat agccaatttg ttcttccagc gcggcaatgc ttttaccaaa tggcgaagcc
    16501 gtcatatgga ttttttctgc cgcccgggca atattattgg tttgcgctaa taaaatgaaa
    16561 ttgcgcatct ttttcgaaat aaaaatgtcc atccccctcc cccgcatcct tgtccgctta
    16621 accgtttcag tcagcctatc cttgatgaaa ccgcgagcaa agataggtga ttacgtcatg
    16681 gttttacaga aaattacaga aaaaggaggc aatatcgggt aaaggcatta gcccgacgaa
    16741 tacgtcgggc tacaagtatt attgtgctgc aggtgtttta gcgggttgtt gatccacagg
    16801 ttctaactgg aagaccacat cgacctgatc atcaaactga atagcggcct gctcgtaagt
    16861 ttcctgggcg gacaccggcg cggcatcggc tttcatcatc cgcaccattg ggctgggctg
    16921 atagttggaa acatggtagc gcacgctata taccggcccc agtttacgat gaaagccgtt
    16981 cgccagttcc tgcgcctgat gaatcgcgtt atcaatcgct gccttacgcg ctttgtcttt
    17041 ataggcatcc ggctgcgcca cgcccagcga cacagaacga atttcgttaa gacccgcctt
    17101 cagcgcgcca tccagcaagg aattcagttt gtctaactga cggagcgtga cttccaccgt
    17161 tctcacagcg cggtagcctt tcaggatact tttaccatcc tgataatcat aatctggctg
    17221 ggtgcgtaaa ttcgctgagc tgatatcttt tttcgcgatc tgattgagtt caaggaagga
    17281 aatgtattgt gcgacgcgct catctgcctg tttcttggct gtagcggcat ccttcgcggc
    17341 cacgttaact tcaatcgcaa gagtggcaat gtctggcacc gcatccacac ttgccgtacc
    17401 ggaggtgaca atatgcggtc catccggcaa ttcgttagcc tgcgctgcca tcccgctaat
    17461 acccattaat gccgccaggg cgataacttt gaacttcacg ttagtctctc cgtgttaatt
    17521 gacttgccca aaattcagga cctctggagg caagcttagc gcctctgttt tatttttcca
    17581 tcagatagcg cctaactgaa caaggcttgt gcatgagcaa taccgtctct cgccagctgc
    17641 aaggctataa accacatgac acatcccact accagattga taatgcgctg tgattttgcc
    17701 gtgcgcaggc gcggtgccag ccaggctgcg agaatagcca gcccaaagaa ccacaggaaa
    17761 gaggcgctaa ttgtcccgag cgcaaaccaa cgttttggtt cgacgtcaag ctgcccgcca
    17821 aggctgccca gtacaacaaa agtatccagg taaacatgcg gattcagcca ggtcactgcc
    17881 aacatggtgg cgataatttt ccatctgcct tgcttcagga cttcggcact agctaactca
    17941 atattactac tcatcgctgt tttaaaagcg ccaaaaccat accacagcaa gaagactacg
    18001 ccgccccagg tgaccagcgc cagcaaccac ggcgactgca tcaataacgc gctaccacca
    18061 aaaatccccg cgcaaatcag gaccaaatcg ctgatagcgc aaagtaaggc aatcataatg
    18121 tgatactgac gacgaatgcc ctgattcatc acaaaagcat tttgtgggcc gagcggcaga
    18181 atcatagctg ccccaagagc aagaccttga aagtaataag aaaacacgtt ggatattccg
    18241 aattaagtat ctgatgcaga gactatatcg cgggattgtt attagcggaa attgataata
    18301 ttaattggca atcagaataa ctaataagag agaggccaga gagtattatc tggcctcagc
    18361 gttgattacg cagctttgtc ttctttcacc cgcttaaagt tcacatccat ttgcgggtac
    18421 gggaagctga taccggcggc atcaaattca cgtttaatac gctccagcac atcccagtac
    18481 acgttttgca gatcgccgct gttgctccag acgcggacca cgaaattaat cgacgatgca
    18541 ccaagttcgt tcaggcgcac agtcatttcg cgatctttca aaatgcgatc ttcagactgg
    18601 ataatattgg tcaggatctg cttaacctga tcgatatcgg aatcatacgc cacgccaata
    18661 ataaattcgt tacggcgaac tggctcgcgg gagaagttaa taatatttcc ggcaataatt
    18721 ttaccgttcg gaataacgat aattttaccg tctgcagtac gcatggtggt ggagaaaatc
    18781 tgcacactca gcacagtacc ggctacgccg ccgaggtcaa catattctcc ggcacggaac
    18841 gggcggaaca tgacaagtaa cacgccagcg gccaggttag acagtgaccc ctgcaaagcc
    18901 agaccaacag ctaagcctgc ggcaccgagt acagcaatga cggacgcggt ttgtacaccc
    18961 acgcgtccca gtgcagcgat tagcgtaaag gcgataatac cgtaacggac taatgcagag
    19021 agaaaatcag caacagtggc atcgatttta cgggagatca tcaggcgatt caccgcgttg
    19081 gaaatcatcc gcgcgataat caaaccaacg atgatgatcg cgagtgccgc cacgatgttt
    19141 actgcataac ttagcagcag cgcctggtta gctaccagcc agcttcccgc gccgtttatg
    19201 ctatcgacaa cattcaaatc ttccattcaa tattcctttt caactgactc aaaatggaga
    19261 aatacactcc gccgccttat gacgggcagt ctgacaaggg taaacaataa agcataattt
    19321 tgccaaatag atcacagatt tctcatctga atcaccttgc ttttggcgaa aaaatccccg
    19381 cagagcgggg ctcagacaga tatcaatgtg cgctttgtca tgccggatgc ggcgtgaacg
    19441 ccttatccgg cctacaaaaa cctgcaaatt caatatattg caggatacgt gtaggcctga
    19501 taagcgtagc gcatcaggca attttgcttt tggcgaaaaa aagacccgca gagcgggcct
    19561 tgagataagc agaaaggaat atcttacaga acgtcgatcg cgttcagttc ctggaatgct
    19621 ttttccagac gagcgatcat cgaagtctga ccggcacgca gccatacgcg cggatcgtag
    19681 tatttcttgt tcggctgatc ttcgcctttc gggttaccca gctgaccctg cagataagct
    19741 tcgttcgctt tgtagtagtt cagaacgcct tcccaggttg cccattgggt atcggtatcg
    19801 atgttcattt tcactacgcc gtagcttacg gagtctttga tttcctgagc agtagaaccg
    19861 gaaccaccgt ggaatacgaa gttcaggctg ttgtgcggca ggttgtgttt cttagaaaca
    19921 tattcctgag aatcacgcag gatggtcgga gtcagaacca cgttacccgg cttgtaaaca
    19981 ccgtgtacgt taccgaagga cgctgcgatg gtgaaacgcg ggctgatttt gctcagctcg
    20041 gtgtatgcgt aatcaacgtc ttccggctgg gtgtacagtg cagaagcgtc catgtggctg
    20101 ttgtccacgc cgtcttcttc accaccggtg caacccagtt cgatttccag agtcatgccg
    20161 attttggaca tacgctccag gtatttagag cagatttcga tgttctcttg cagagattct
    20221 tcagacaggt cgatcatgtg agaagagaac agcggcttgc cggtagcagc gaagtgtttt
    20281 tcacccgcgt ccagcagacc gtcgatccac ggcagcagtt tcttcgcgca gtggtcggtg
    20341 tgcaggataa ccggaacacc ataatgttca gccatctggt gaacgtgatg cgcaccagag
    20401 atcgcgccca ggatagcagc accctgcgga acgtcagatt tcacgccttt accagcgata
    20461 aaggaagcac caccgttgga gaactgaacg ataaccggcg ctttaacttt agcagcggtt
    20521 tccagtacgg cgttgatgga gtcagtaccg acgcagttta ctgctggcag tgcgaagttg
    20581 ttttcttttg ctacctggaa aactttctgt acgtcatcac cagtgattac gccaggtttt
    20641 acgaaatcaa aaatcttaga catgtctctt gtcctgtatc gtcgggcctt atactcgtca
    20701 tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag
    20761 ctcctgggga ttcactctct tgtcgccttc ctgcaactcg aattatttag agtatgaaaa
    20821 aatgcgtgct ctaaaagcgc gctgaaacaa gggcaggttt ccctgccctg tgatttttta
    20881 cttcttagcg cgctcttcga gcatcgctac tgcaggcagt actttacctt ccacgaattc
    20941 gaggaatgcg ccgccgccag tggagatgta ggagattttg tcagcaatgc cgaacaggtc
    21001 gattgctgcc agagtgtcgc cgccgccagc gatggagaac gcttcgctgt ctgcgatagc
    21061 gttagccacg atttcagtac ctttgcggaa gttcgggaat tcgaacacgc caaccggacc
    21121 gttccacaga atggttttcg cattcttcag gatttcagcc agttcctgag cggaagcatc
    21181 accgatatcc aggatctgct cgtcagcttt cacatcgtta acagatttca gggtagccgg
    21241 tgcagtttca gagaactcgg ttgctacgcg aacatcagac ggaaccggga tgttgcaggt
    21301 ggtcagcaga cgtttagctt cgtcaaccag gtcagcttcg tacagggatt tacccacatc
    21361 gtggccttgt gccgcgataa aggtgttagc gataccacca ccaacaatca gctggtcagc
    21421 gattttagac agggagtcca gaacggtcag tttggtagat actttagaac caccaacgat
    21481 agccaccatc gggcgagcag gttctttcag tgctttaccc agcgcgtcca gttcagctgc
    21541 cagcagcggg cctgcgcacg caacgtcagc gaatttaccg ataccgtgag tagaagcctg
    21601 cgcgcggtga gcagtaccga atgcgtccat tacgaacacg tcacacagtg cagcgtattt
    21661 tttggacagg gtttcgtcgt ctttcttctc gcctttgttg aagcgaacgt tttccagaac
    21721 aaccagttca ccttcagcaa cgtcaacgcc gtcgaggtaa tctttaacca gacgaaccgg
    21781 gttagacagt ttgtctttca ggtagttaac aaccggcagc agagagaatt cttcgttgta
    21841 ctcgccttcg gtaggacgac ccaggtggga agttaccatc acttttgcgc cttgtttcag
    21901 ggccagttcg atggtcggca gagaagcacg gatacgcgcg tcgctggtta ctttcccgtc
    21961 ttttactggt acgttcagat ccgcacggat aaatacacgt ttcccagcaa gatccagatc
    22021 ggtcatctta attacagaca tggtgaatcc tctcgttgat tctaaaagtt ttgcagacgc
    22081 tgcttgcgtc ttacctgaaa gcaacagtag ccatagctaa cgtcgtgtcg agcattcggt
    22141 tagcaaagcc ccattcgtta tcgcaccaga ccaacgtttt gatcaggtgt gcgccactga
    22201 cccgggtttg ggtgccatcg acaatggcac tgtgcggatc gtggttaaaa tctacagaga
    22261 ccaacggcaa ttccgtatag tcaactatac catgaaatgc accttgtgct gctttttgca
    22321 gcaacaggtt gacttcattg gcttttacag gtttcttcac cgtcacgctt aaatcgattg
    22381 ccgtcacatt tatggttggc acacgtaccg caatcgcttc aaagcgatcg ttaaattgcg
    22441 gaaaaaatcg tgtgataccg gcggccagtt tagtatcgac cggaatgatc gactggctgg
    22501 ctgcccgggt gcgacgcagg tcaggatggt atgcatcaat aacctgttga tcgtgcatgg
    22561 cggagtgaat tgtggtcact gtgccggact caataccgta cgcatcatct aacaatttga
    22621 tgacgggaat tatgcaattc gtggtacagg aagcgttaga aacgatgcgg tgttccgcac
    22681 gaagttgatc ctgattgacg ccgtaaacaa cggtcgcgtc gagatcgtta ctgccaggat
    22741 gtgaaaagag cacttttttg gccccggcgg caatatgcgc ttcgccatgc tcgcgggagc
    22801 catatacgcc ggtgcagtcg aggactacat caacgccaag ttcacgccag gggagcgatt
    22861 gcagtgaacg ttcatgcaat acgcggatgg cgtcatcacc aacaaaaagt tgatcgcgtt
    22921 cctgtcgtac ttcccatgca aaacggccat ggctggtgtc atatttcaac aaatgcgcca
    22981 tgcccgcagc atccgccagt tcgttgattg ccaccacggt aatttccgcc cggcgtccgg
    23041 attcatacaa agcacgaacc acattacgcc cgatgcgacc gaagccattt atcgctacgc
    23101 gtacgatcat agatctcctg caaggttttc cctgagcaaa tttgccagac agagtaatcc
    23161 agcaaatcgt ccggggaaac cttacctgtc gcaaactgcg actgattggt taattgtcga
    23221 acatttaatc gactgaaacg cttcagctag gataagcgaa acgtggaata aaaggaatga
    23281 ttgtccagtc aaagaaagca attatctgac ttacgtcacg ttttcagccc cgcccctgtg
    23341 gaatttattc cactttgcag aatcacgaat aagcgtagca aaatcttttc gttgtgccgt
    23401 cacaaaccct gacttatatc agaatatgac tgatgcagcg tcgatattat ccgcaatacg
    23461 tccgagtttc ggagtactcc aatgatgaca ttgccctacc catattcttc ctccgcacgc
    23521 ttttacgcac tgcgcctcct ccccgggcag gaagtttttt cccagctaca cgcttttgca
    23581 cagcagcatc aacttcatgc cgcctggatt gcaggctgta ccggtagcct gactgacgtc
    23641 gccttgcgct atgccgggca agaaggcacc acgctcctga acggtacatt cgaagtgatc
    23701 tcactaaacg gcacgctgga acagagtggt gagcatcttc atctctgcgt ttccgatcca
    23761 cacggcacga tgcttggcgg tcatatgatg cccggctgta ccgtgcggac cacgcttgaa
    23821 ctggtgatcg gtagccttga ggaactggcg tttagccgcc aaccttgcgc gctttctggt
    23881 tacgacgagt tgcatatttc ccccgttaaa taactacttt caaagaggtt ttcttatggc
    23941 gcgtagccac ttttccagtc aggcactggt gttgatcgtt atatctatcg ccatcaatat
    24001 gatcggcggg caactggcca gcatggtcaa attacctata tttctcgact ccatcggcac
    24061 acttattagc gcggtactgc ttggtccggt gatcggaatg ctgaccggct tactgaccaa
    24121 tttactctgg ggattgctga ccgacccgat cgccgccgca tttgcacccg tcgcgatggt
    24181 catcggcctg gtcgccggtt gcctggcgcg agcgggttgg tttcgcaccc tgccaaaagt
    24241 cgttgtcagc ggcgtgatta ttacgcttgc ggtaacggtc gtcgctgtac cgctgcgtac
    24301 tgcgctgttc ggcggtgtca ccggtagcgg agccgatttg ttcgttgcct ggatgcactc
    24361 gatggggcaa aacctggtgg agtcggtggc gattacggtg ataggcgcga acctggtcga
    24421 taaaattctt acagcggtga ttgtctggct tttactgcgc cagctgccga ttcgcaccac
    24481 gcgccatttt ccggcaatgg ctgccgtgcg ctaaatgcat ccatttacct cattaacact
    24541 gtgggcattg gcggcctgca ccacgcttat cctgcccgcg cagaccattt tgccgatcta
    24601 tagcgcggcg acctttttct gccttattgc gctaaaggcc acgcggcggc gggcaaaata
    24661 tgtcgtctgg ctgatgttct cgctgggagc cgggctgtgg ctggtacacg gtggctggct
    24721 accagaatgg ctaagcggca cgccacgctc gccagaacgg tggtcgcatg ctattacgct
    24781 ctggttgcgg attctggcga ttgtttcaac ctcacagttg tggatgcagt acgtccccgt
    24841 gcaatgtttt attcgcgctc tgtttgcttc tcgcctgccg ccaggcgtcg cttatctgtt
    24901 tgccgggccg ttattggtcg ttgagcagtt aaaacggcaa ttagcgatta ttcatgaagc
    24961 gcagcgtgcc cgcggcgtgc cgctggatga gggctggtat cagcgtttac gtgcgatgcc
    25021 cgcgttaatt attccgctaa cacacaatgc gcttaacgat ctggctgtcc gtggtgcggc
    25081 gctggatatg cgggcatttc ggatccacaa ccgacgaaca acgttatggg caccagccga
    25141 tagcacccta caacgcgtag cgcgttacac catgatcttg ctgatgctga cagaattcgg
    25201 agcgtggata tggttacgtt agaacagttc cgttattgcc cgacacactc aacgcatcct
    25261 ccgttctgtt atgactttca ctatgtcaaa ccggggatgg tcgccatctt tggtgacaac
    25321 ggcagtggca aaagtacgct ggcacagctg atggcgggct ggtatccgga ctttctgcca
    25381 ggagagataa caggcacggg gacgctcctc ggtacgccca ttgggcatct cccgcttaat
    25441 gaacaatccg ccaccatcca gctcgtccag caatcgccct atttgcagct ctccggctgt
    25501 acctttagcg tggaagaaga agttgccttt gggccagaaa atctgtgtct tgctgaagca
    25561 gagattatgg cgcggattga tgccgccctg actctgaccg agtgccagcc gttgcgccat
    25621 cgtcacccgg caacactctc cggtggagag acacagcgcg tggtcatcgc ctgcgccatc
    25681 gcgatgcagc caaagctctt aattcttgat gaagccttta gccgcctgac tccgcaggcc
    25741 agcgaaatgc tgttgcagcg cttacaacac tgggcgtttg aacgtggttc gctgattatt
    25801 ctttttgaac gtcatcgcac acctttcctg aactactgcc agcaagcgtg gcaattgaaa
    25861 aacggagcgc tccagccact atgttaacgt taaataagat atcttatcgc tggcctggcg
    25921 cggcaacgga ttgtctttgc gacatttctc tgcaactcaa acaaggtgaa tggttggcgc
    25981 tgaccggcga caatggcgcg ggaaaatcga cgctgttacg agtaatggca ggtcttctta
    26041 cgcccacagc cggtacggtt atgctgcaac aacaggcgat gaaaaacctg aaaaaccgcc
    26101 agcgcgcggc aaaggttggc gtactgtttc aggaagcgga aaaccaactt tttcacagca
    26161 ccgtcgctga cgagatcgca tttggtttga aactgcaaaa atgcccggcg gatgaaatca
    26221 ctcaacgtac ccatgctgcc ctgcaatgtt gtcagttagc agacactgcc aacgcgcacc
    26281 cgttggatct gcatagtgcg cagcgacgga tggtcgccgt cgcctgcctg gaagcactct
    26341 ctccgccact attgctgctg gacgagccga gtcgggattt tgacgaaaat tggttgagcg
    26401 tgtttgaaag ctggctggag aagtgccgcc agcgaggcac gagcgtcgta gcaatcagtc
    26461 atgacgccgc atttacccgg cgtcatttct ctcgtgtggt gcgactggaa gatggtctaa
    26521 tcaggaacgt taatccacca gacgatattc acccgtagcg gtcatcatta aggtcaaatt
    26581 tgcaggcaga ctctcttcca gcacccggcg aacattcggc ccgtcggtac ggtcataaaa
    26641 agcttctgcg tctgccagcg atacccctcc cgccagcttg cgccctacca gtcgttcccg
    26701 caacgcagta gctggcgcgt tgataaagat cgagaaatca caaaattgcg caagctggca
    26761 ccacttctcg tcgtccagca acaaccagtt tccttcgacg atgacgagtg gtgcggtaac
    26821 gtgcaacgca tcttcaacag gatcatgctt ttgtcgatcg tactgcggcc acgtacaatc
    26881 tccttccacg acctggcgca gattttccgc cagtttcaca acgtcaaatg tctctggtgc
    26941 gcctttgaag gggcgcaatt gatgcgcgtc cagccagcta ttgtaatggt gaaaaccatc
    27001 catcgggagc gtctgaatag cgggcagttc cgggtcttgt tgcgcgagat attcccagaa
    27061 ggtagttaag gtagatttac ccgtccccgg cggagcgcaa agaaaaacca ccgtacgtcg
    27121 ctgcgaactt accgcctgta aggccgccag catacgcagt agcggtttat gaacattctc
    27181 aatttcatca tcagagtact gagcctgtac ctttagtcca ttcaccgtca gttcgatatt
    27241 cacggcgtta attcctttaa agttgtcgtt actgccagcg tcagcgccgt tttgaccatt
    27301 ccgctaaaac gcgcctctga ccagacggcg aaatcggcga acttcatcgc tttcaacgcg
    27361 tcatagagcg tcgcattgca ggttatgcta ttgacgttgc tgataaaatc ccaggtcatg
    27421 tcatcggcaa atccgggcgt ttcatcctgt tcgttcaggt aatgccagaa ctgggaaaaa
    27481 tgcgtgatat cggcgtacag ccatttatcc attttcccca gcgcataaat caaacgcagt
    27541 gcaacatcaa tatcgtcaag cgggccgctt tgggcgagta acggcttcac tgcatattcg
    27601 acgatctctt catcgttatc gacaaacagc gacggcagaa actggcgtat cccctggagc
    27661 aaaatagaat gcgcagtttt cataaatgaa aataaattgt cctgtgcatc cagttgctca
    27721 agcacatcat cttctgtcag cgtcgccatt ggtcttccga tagatcacca tcaatgcagg
    27781 gagtctatta tattacacgt ttggtccgcg ccgccgatca gtaatgtctg cgtccgcacc
    27841 ccattcgccg tctgttggat accgttcacc aactcgccgc ccgtcacgcc cgtagcgcta
    27901 aagaggatat cgttccccct caccagttca tcgagcgagt acacacggtt aacgtcaacg
    27961 cccattgcct tacaacgctt gcgctcctgc tctgcaatct gccgattttc cgtgtaatcc
    28021 cctttagcct gacaaaagtc aatcagctcg gcctgcatat ccccacctaa tgctttaacg
    28081 gcacaggcag aaatcacgcc ttctggcgca ccgccgatgg tgtacatcac gtcgtaagga
    28141 ttatcctgcc agcaggtcag cacgctggcg gcgacatcgc catccggcag agcgaaaacc
    28201 ttcacgccca gctgtgttgc ttcttcaatc gcggcactca ggcgcggttt atccagcgta
    28261 accatgcgca gcttgtccag cggtttgcct aacgcccgcg ccacgttgcg caagttgtcc
    28321 gccagaggca aagacagatc aatcgccccg gcagccagtc gattaacaac cagctttttc
    28381 atatacatat cgggcgcatg taacaggcta tctcgagggg cgaaagccat gaccgccagc
    28441 gcattgctct gccccatcgc taccatccgc gtgccttcaa tcgggtcaac cgcgatatcg
    28501 acttctggcc catcgccttt gcccacctct tcgccaatcc acagcatcgg cgcatggtca
    28561 atttctcctt cgccgataac cactcgtcca cgaaaagcga cgtcgtttaa tgcctggcgc
    28621 atcgcggtga ccgccaggcc atcaatttta tttttgtcgc cacatccggt ttgcggccag
    28681 gcagccaacg ctgcctgttc cgtaacgcga aataatggcc acgccaggga catcattgcg
    28741 cggcctcccc aatatcaacg ccaaattgcg ccagcagata tttctcggcc tgctcattcc
    28801 agatgccgtg cgtttcctca accagccgcg ccagctcttt aaacagcgga tcggttttgc
    28861 ctttttcggc aaaatcggca atggcggtga gcggcatggt cacgccgtta taaatgagtt
    28921 ttttaccgcc agggatatcc ggcagattga gcacggtatc tggcaccgca tccagcccac
    28981 cgatatgcgt gaccataaag gacggctgca actgccccgt ggcgcttaac gcaatcgcct
    29041 ctttcatgtc gtccgttgaa ccgccggatg taccgacgac gtgcgtgctg ttgtaatgga
    29101 cgttgtagaa attaaacggc actttgaagt ttttatccgt cggcccggca aagaagttca
    29161 gacagccatc ttccgccagt aattcgtcag ccatctcaac gacagcaggc accgccgcat
    29221 aaacaaaaat gtcatcgaac ccgacatctc ctgtcagcgc ccgcagcgtc tggaccggat
    29281 cgctcatccc tttggtattc acatacacca gctcaatgcc tttactggcc gccagatcca
    29341 ccggcagcag cttctgcacc tgcgccagac gtttgtcgtc gatatcgacc accaccaccc
    29401 gcgacggttg tatgccgccg ttaatggcgt aatcgatagc gccaatgccc atcggacctg
    29461 cacacgccag cagtgcaata ttgccgccag gtttgacgcc catgcgatgc tcataaacat
    29521 attgcgtggt gtgataattg gcatgataag caccaataat gcagcacata ggctctgcca
    29581 gcgacgccgc agcaaaataa gagccgtgat acggtaatac gcagcccaaa ttaatggcga
    29641 tttcgggaat aatcatataa gtggcattgc cgccaaaata ttcgtagctg tagcccgctg
    29701 aatatccgct tggtaacccc atcgctggtt gcaatacaaa acgctggcct tttttatatt
    29761 tgccagtgag atttttaccc acttcgacaa taaccccggc acattcatgc ccggtaatga
    29821 ccggatgatt ttctaaatcg tcgggtacgc gtttatgttc actaccgagt aacgcagctt
    29881 tccaggtgga taaacagacg ctgtcagaaa ttacactcac cagtaattca ttatcggtaa
    29941 tttctggcag ttcaaattcg cgcagacgga catcccgctt gccataaata gcagcaactt
    30001 tggttttcat ttttacctct gtaatcagtg tttatgttcg gcggttaatt gattaaataa
    30061 agtgtcgagt tttggatcgc caatatagtt attaatcaat atcagcggtt tatccgtaac
    30121 acgtttcacg cgcccttcca gactggcatg agtaacgacg atatcggcat ccgcaggcac
    30181 gttttctatc gcgtaatgtt ttacttctat tgccagcccc gctttttcca ggcgtttacg
    30241 gaaggtggtc gcgcccatcg cactggagcc catccccgca tcgcaaacaa aggcgatacg
    30301 cttaaccctg cttaacgaga atgcaccctc ttgtttcatc gccttaaccg cattggctga
    30361 ctgagcaaac tcatcttcgc tctccgtttc caccgttttt tccatcttca gtatcagcga
    30421 agtaatagca aaggacacca gggtacctac cgtaacaccg gcaattgtcg ccaggaacga
    30481 gcctttcggc gtcagtgcca ggtaagcaaa gatagacccc ggacttgggc cagccaccag
    30541 accaccgtcc agtaagttaa acatccaggt accagacata ctgcccgcaa tcatggcaat
    30601 aatagtcagc ggcttcatca gcacatatgg gaaatacagt tcgtggatcc caccgaggaa
    30661 gtgaataatc atcgccccgg gcgcagaacg tttactcatc cctttaccaa acaaggtaaa
    30721 cgccagcagc agccccaggc ccggacctgg gttagaggcc accataaaga agatggattt
    30781 accgttaacc gaagcctgtt gcattcccag cggataatag acgccctgat cgatcgcatt
    30841 attaaggaaa agcactttcg ccggttcatt gatgactgac agcagaggca gataacccgc
    30901 atgtaccagc gcctcgatac actctttgac gaaagtattg gcaattaaca ccgccgggcc
    30961 gatcacttca aaacccagca gacaaaggag catccccgcg atacctaatg agaagttatt
    31021 gataaccatc tcaaaaccgg caggtatgcg tttttccagt gccttatcga catattttat
    31081 gaccaaccca ccgagcggcc ccatgatcat tgagccaagg aacatcggga tctctgcgcc
    31141 aacgatcaca ccaatagtac ctattccgcc catgaccgcc ccgcgtttac cgccgaccag
    31201 atgaccacct gtagaaccaa tcatcacggg caataaatag gtaatcatcg ggccgacaat
    31261 tttggcgaaa tgttcgttag gcagccaacc ggtgggaata aataacgcag taataaaacc
    31321 ccaggcaata aaagcaccaa tattggggat gaccattgca gtcaaaaagc ccccaaaagc
    31381 ctggaccttt gcacgagcag acttgttttc cataatatta tcctgtagag gagagagtaa
    31441 ttatccgcgg ctgataatgt ccgctaattg tttttctgat gatgctgtta agagttgttc
    31501 gagtatttct tcttcgcaca gtaattcact taacgcctga atagcgccaa tgtgcgaatc
    31561 ggcatcggca gcagagagtc cgatgagtaa tttaattggt tcgtcattcc ccggaaaata
    31621 aacaccttgt tcaagtaatg tcaaagacat cccggtttta agcgccccac attccggtcg
    31681 cgcatgaggc attgccacgc ctggtgcgag aatataataa gggccattgt taatggtgga
    31741 atctttaatt gcctgaatgt aattctcgct gatatagttt ttatccagca atgataccat
    31801 cgagaaatcg atagcttcct gccaatcttt tgctgaatgt ataactgaga ttgatgattc
    31861 tggaaaataa tcactaagcc gcataaatta tccttatttt atttttaggg taccgtccgg
    31921 cggtaaaaat aaaatactcg tgcggtttaa tgaaagcaat tgagcaggga aaccgatgtt
    31981 ttgaaaacag tgaattatta atttcttata taacattgag ttatagatat aacaacaaac
    32041 gtcaatacgc atatcgtgat gcatattaca taattgatat ttattgcata taaatatttg
    32101 tgatctacaa cacgctttat ctattgcttg tccgcaaacg gacatatcaa ggtaataaaa
    32161 aaggtcgccg aagcgacctt tttacccgaa atgctaatta cagcagttct tttgctttcg
    32221 caacaacgtt atcaacagtg aagccgaact cttcaaacag cagctctgcc ggagcagatt
    32281 caccgaaggt ggtcataccg acgatagcac cgttcaggcc aacatacttg taccagtagt
    32341 cagcaatacc cgcttctaca gcaacgcgtg cagtaaccgc tttcggcagt acggattcac
    32401 ggtaagcagc atcctgcttg tcaaatgcgt cggtagacgg catggacacc acgcgcgctt
    32461 tcacgccttc ggcagtcagt ttttcgtagg cagcaacagc cagttcaact tctgaaccgg
    32521 tagcgatgaa aatcagttcc ggctgaccgg cgcagtcttt cagcacataa ccaccgcgcg
    32581 cgatgtttgc cagttgctct tcagttcgtt cctgctgcgc caggttctga cgggagagga
    32641 tcagtgcggt cgggccgtcc tgacgctcaa caccgtattt ccacgcgacc gcggattcaa
    32701 cctggtcaca cggacgccat gtagacatgt tcggggttac gcgcagagaa gcgacctgct
    32761 ctaccggctg gtgagtcggg ccatcttcgc ccagaccgat ggagtcgtgg gtgtaaacca
    32821 tcacctgacg ctgtttcatc agcgcagcca tacgtacggc gttacgtgcg tattccacga
    32881 acatcaggaa ggtggaggtg tacggcagga aaccaccgtg cagggagata ccgttagcaa
    32941 tcgcggtcat accgaactcg cgaacaccgt agtggatgta gttacccgca gcatcttcgt
    33001 tgattgcttt agaaccagac cacagggtca ggttagacgg tgccaggtca gcagaaccgc
    33061 cgaggaattc cggcaacagc ggaccgaaag cttcgatagc attctgagac gctttacggc
    33121 tggcgatttt cgccgggtta gcctgcagtt tagcaatgaa ctcttttgct ttcgcgtcga
    33181 agtcagacgg catttcgcct ttcatacggc gggtaaattc agcggcttcc tgcggataag
    33241 ctttcgcgta agcagcgaat ttctcgttcc atgcggattc tttcgcctgg cctgcttctt
    33301 tcgcatccca ctgagcatag atttcagacg ggatttcgaa cggtgcatat ttccagccca
    33361 gctgttcgcg ggtcagggca atttcagcgt cgcccagcgg cgcaccgtgg gagtcgtggg
    33421 taccggcttt gttcggggaa ccgaaaccga tgatggtttt gcacatcagc agggacggtt
    33481 tgtcagtcac tgcgcgcgct tcttctactg cgcgtttgat ggatgccgcg tcatgaccgt
    33541 cgatgtcgcg aataacgtgc cagccgtaag cttcgaaacg cattgcggtg tcgtcagtga
    33601 accagccttc aacgtgacca tcgattgaga taccgttgtc atcgtagaac gcaatcagtt
    33661 tacccagctt cagcgtaccc gccagagagc aaacttcgtg ggagatgcct tccatcatgc
    33721 agccgtcgcc catgaaggcg taggtgtagt ggtcgacaat gtcgtgacct ggacggttaa
    33781 actgcgccgc cagcgttttt tctgcaatcg ccataccgac tgcgttggca ataccctgac
    33841 ccagcggacc ggtggtggtt tccacaccag cggtataacc tacttccggg tggcccggag
    33901 ttttagagtg cagctgacgg aagtttttca gttcttccat cggcagatcg taaccggtga
    33961 ggtgcagcag gctgtagatc agcatggagc cgtggccgtt ggacagcacg aagcggtcac
    34021 ggtcagccca ggacggattc tgcgggttgt gtttcaggaa atcacgccac aggacttcgg
    34081 caatgtcagc catacccata ggggcacccg ggtgaccgga tttggctttc tgtactgcgt
    34141 ccatgctcag cgcacgaata gcattggcaa gctctttacg tgaggacatt ttgactccag
    34201 atcggatgat gaagggcacg cccttaacga cttgacgaca gcgcgttttg ggctacgccg
    34261 gaaaatttgc caacaattta ccgcaagccg cgcgtcatgt acatggaaca tccttttgcc
    34321 gcttcagaaa tctctggatc atgctcgcat gttgcgcaat ctactcgccc gtccgctgcg
    34381 cttttcctta tactgagact gagcgtcgat tcacctgcaa acggcgcatt tttagaataa
    34441 tcctgacctt gtgcggaaga gaaaacatga aaattcgcgc cttattggta gcaatgagcg
    34501 tggcaacggt actgactggt tgccagaata tggactccaa cggactgctc tcatcaggag
    34561 cggaagcttt tcaggcttac agtttgagtg atgcgcaggt gaaaaccctg agcgatcagg
    34621 catgtcagga gatggacagc aaggcgacga ttgcgccagc caatagcgaa tacgctaaac
    34681 gtctgacaac tattgccaat gcgctaggca acaatatcaa cggtcagccg gtgaattaca
    34741 aagtgtatat ggcgaaggat gtgaacgcct ttgcaatggc taacggctgt atccgcgtct
    34801 atagcgggct gatggatatg atgacggata acgaagtcga agcggtgatc ggtcacgaaa
    34861 tggggcacgt ggcgttaggc catgtgaaaa aaggaatgca ggtggcactt ggtacaaatg
    34921 ccgtgcgagt agctgcggcc tctgcgggcg ggattgtcgg aagtttatct caatcacaac
    34981 ttggtgatct gggcgagaaa ttagtcaatt cgcaattctc ccagcgccag gaagcagaag
    35041 ccgatgatta ttcttacgat cttctgcgcc aacgcggcat cagcccggca ggtcttgcca
    35101 ccagctttga aaaactggca aaactggaag aaggtcgcca aagctcaatg tttgacgacc
    35161 atcctgcatc cgccgaacgc gcccagcata ttcgcgatcg tatgagcgcg gatggggtta
    35221 agtaagtcag acataccgat ctacaacata attgtaggtc ggtaacgcct gaatccccga
    35281 tcagagtagt gatttcactt tatttagcaa aacggctaca tcatccggtc gggtatatcc
    35341 cgtatcaggg tctataatcg agctgtaaac atgtggcata atgcgcggca cccccgcctc
    35401 cagacaagtt ttcagaatca cactgaagtt atccaaatca atcccgccag tcggctcaat
    35461 cagcgtcata ccattgcgcg ccgccgtggt cgccagcgcg tatagctccg gaagcgacct
    35521 ctcgccgccc atcggaaaga acttcgccgc gtgtgcgccc atatctagca tcattcgcac
    35581 cgccgtatcg caggagacgc gggcaggcgt accctggctg ctgctaacgc cggtggaaat
    35641 cagcacttcg cccggcgtcc ccgtcgggct aaccagcgcg ttgacatggg tctgctcacc
    35701 gcccgtagcc gcaagcgcgc ctgccgcgaa accacaaccg gtaaaggtct ggttaacgtg
    35761 tgctggatga atctgcgccg caatcatcgc agctttgtaa tactgcgccg ggtcacccgc
    35821 ccccagacct actgaaatgg acggtatttc cgccatccaa cgtttaactt cctggatccc
    35881 ctcatccacc gaggagaact gcgcggaaag cacgccaatc actgcgtggc cttcggcggc
    35941 gtcaaagatt tcacgggcat tggcgatatc tttcgccagc acattaattg ccacgcgatt
    36001 acgatagaaa ttaatctttt gcatgatctg caatctcctt cagtcgcgcc acgataagtg
    36061 ccatttcccc ttccgccacc gtacgcggat caaggctaaa aacgccctgg tggaggttat
    36121 accgccgtgc ataaatcgcg atatagccac cacgcagttg cgcttcgacg acgcgagcgt
    36181 caactcccaa ttcctgcgca tttactcgaa tacgaatacg ccagattgca cgcccggctt
    36241 catcctgctc aatatcagcc gttaacccgt gaatcgcaga aatcgcttca accactggct
    36301 ggagctgttc cgcggtgatg actgcctgcc cctggtgata attctccagc gcgtaaacca
    36361 gcccaaccat gttctccttg ccgattttca tcgccctggc aatgccgtga tgctgcgctt
    36421 tgcaggctgc aatccagatt tttttaccag tgataaaccc ggaggtcggc gcgttaaagg
    36481 cttttgcgcc gctgtagacc accatatccg ctccgctcac cacccaggca cgtaaatcct
    36541 cttcggcggc ggcatcgaca atcagcggca ggttgtacat ctgcgcgaca tgtgcgaagt
    36601 cctcgatgtt caacattccc ttctgcacgc agtgatgcga cttcacatac agcagcgcgg
    36661 cggtcgcgtc ggtaatggca ctctccagct gccagagagc cgccaggtta cttgaaccca
    36721 cctccacaac gcgcccgcca cctaagcgga tcgcgctgag gatcgatgca ccgtaatcga
    36781 cgttatggcc gcgcagcatc accacttcat tggccatgcc ggagctatcc ggcatgagcg
    36841 ccacccgggc cttgtcgccg cgtgtaatgg cggcggcgac agcgatggcg atccctgctg
    36901 aggcacagga ggtaacgtag ctatcctccg ccccggtaaa gcgagagacc agctcgccgg
    36961 ttttgtccac cagctggtca atctccacga aagaggatgc cgcgcgcgcg gtggcctgca
    37021 tcacctccgg cgcgacgctg gagacgccga gaatcgtcat cttgccgcag gcgttaatca
    37081 cctgtttcag tcccagttgt tgatagatat tctgcgtcat ggcttacagc actcccagca
    37141 gcgagcagac cacgctcaac gccacaatcg acagcagaat cgtggtgtaa cgcggaccct
    37201 ttttcaccaa atagaagtag atggcgaaca ctgccgccag cggcagcagg ccgggcgcga
    37261 tggagtccag aatctgctgt accaccactt cagagccttt cagtgcgcta atttttaaag
    37321 gtgaggttat cttgacaaag ctcgcggata gcgcgcccat catgataagc cccagtacgt
    37381 tagcgccgta gataagctcc ttgatgcgcc ctccctgcag cagactgata atcgaatcgc
    37441 gccccagcgt atagcccttg tgcaccatgc cgtagctgat tgccagcgta atcgccgggt
    37501 agagaatcag cggcaaaata ccgcccatcg cgctgccatt ggcggcgaac gggataaaaa
    37561 tagcaatcaa cagcggcatc accgccgccc agatgatgga gtcgcccatc ccggccagcg
    37621 gccccatcag gccggtttta atcccggtga tagaggcatc gttaatcgcc tcgccgcggg
    37681 tcttttgctc ttccatggcg atggagatcc cctggatcac cgcgccaaac gtctgctctg
    37741 agttgaagaa gttcagatgg cgttttaacg cctccacctg ctcctccttt tgcggataga
    37801 gctttttgat aatcggcgtc atcgaggcgc agaaaatcag gctctgtaaa cgctcatagg
    37861 agctggaaac ttccgcgccc agccagtaga taaaccacgc tttggtgata tccgctttgg
    37921 tcagcgcccc ggactcccgc gcgcgctcaa ccagttcatg ctgcattacg tcgtcgctca
    37981 ttatgccgct ccttcatttt tcgccaggcc tttaatcagg aaggccacgc aggtgccgaa
    38041 aatcgccatc gccatgatgt ccaccttgag gtacagcacc gcgaagaatc cgccgataaa
    38101 ccacggcagc aggctctttt taccaatcac cataatggtg atggcgaagc ccagcgccgg
    38161 gagaatcccg cccatgattt caaacgagtg ggtcagccag tgcggcatca gcttgaggaa
    38221 actttccact acgttctggc cgaagtagtt ggcggcgaac accactggga aacgtagtac
    38281 cagccccagc agcgccgggt agagaaaggc gcagcgcata atggctgaca tattggccgt
    38341 ttccgcgtgt ttatcggcca tatgcaccca ggccgcattt agcgtgcgac gcaactggtc
    38401 gaggaatacg ccgataacgc caaatggaat cgccagtgca atcgccaggt tcgggtccat
    38461 attggcctta acggcgatag gaatggcgat acaggccgcc agcgccgggt ctgacggcac
    38521 attgccgcct ggcgttgagg tcacacccag atagaccaac tgcatccccg cgccgataat
    38581 catcgccgca ggcatattgc cgagcagcag gccgacaaag accgccacca ccactggctg
    38641 taacagcatg gcggagaagg tatagccaag gcgcaaccgg gcaaaccagt aatacagccc
    38701 catcaggctt gcaaaaagta aggtatccat aatttgttat cccatgttca gagggtcaga
    38761 attttttcag gatatcgtcc agcgactgcg gtttatcttc cgggatggtc tggaaaacag
    38821 ccttaatgcc gcgacttttc aaatcgttaa gaatgccaac gtctttttca tccagcgtta
    38881 tgttctggaa caccgcttta cggtttggcc cgccgcctaa accgccaacc tgaatatcag
    38941 ccacgtcaaa gccctgctgc accgcttcct gaatcgccgc cagcgacggg aacagcacca
    39001 gcacgttgcc gtcgcccagc tggttctctt tccacgcggc cgcgaagctc tggttgctaa
    39061 agcagtccac tttgatattc ggcggcgcgg ccatcaggta gatatttttc ataaacggat
    39121 cggcgtccag ctcgtcgctg accaccgcaa tacggttggc ctgagactgc cctacccact
    39181 tcgtcaccac ctgtccgtgg ataagacggc tgtcaatgcg acataacacg atgtttgcca
    39241 tacgattttc cttactgttg taattgacga atatgggaga cgacgtccag gcaactgttg
    39301 cgcccagctt ccaccagatc ggccacgcag gcggtcagtg ggccatgttc gcgcttgtcc
    39361 agcgcctcca gcagcagcga ggcattaagc ccggagatca ccgccaccgg ataatcggcg
    39421 ctcagccgcg ccgccacgtt agaggtggtg ccgccgataa aatcggtcac gataagcgaa
    39481 ccttccggca tggttttcac cacggcttcg acgcgctggt aaaactcgcc cagcgtatcg
    39541 acgggcatca gcgcgatttc cgtcacgcct tcgatttcgc ccatcaccat gcgcagactg
    39601 ttgcagagct gcagccccca gccgccgtga gttagcagca gaatttcagg taacggatga
    39661 ataggtacag ttgtggtcaa aatagcctcc tttgctccag tgtttacatt tcatcaggag
    39721 caaaggacgt gccagaattt gtgtcacgca cagaggaaat gagattggat ggggcgttaa
    39781 gccccgtaaa ccaggggatc agctgtagag cagttcgtag atataaaaat attctgcgtc
    39841 cgataaacgg atagcgtaag cctcttcaat cggcaaaaag gaggatttga tgacgctaaa
    39901 ggcacgagcg tccagatcaa gccggttttc tagcgccatc tgtaacggtt tgcggttaat
    39961 cacgatacgc tcgaccatgc agcagcagtg gatcagaaaa cgtagtgtca cctggcggct
    40021 cggtttcagg gagagcgtgt tggttaagta gttcagaaca tcctcgatct ctttcagaat
    40081 tcgctgcggg ttgagcaccg aaatatggtt gatgatgctc tccatggtca gcgcgctgat
    40141 aaagcgcatg gcgctgcgct ccatctccag tcggcgctcg ctgcttgaga gatccggcgt
    40201 cagcaggctg agcaccagct ccggcccttg ctcggaaaac agttcctcca gcgagataaa
    40261 cggtatatcc ggcagtcccg gctggaaggt gccgacgatc cctgccagcc gttcactggc
    40321 attaagcgcc tgctgaatac gctccaggct gcgcacctcg ttatagtcga gaatcaccat
    40381 acgcgtgtcc tgcgacatca gctcgccaaa gctctcctcc agcacctttt tgatcttttc
    40441 cgccgtcccc attccggtta tacatgagat aaccagcact ttgccaccgc tctcttgctg
    40501 cggcgtgcag agccgacaag ggatattttt actctgcata agcacagcca attgcgatag
    40561 atcgccagtt tcgtagctta aatccagccc aacctccagc aggctggtca gagtgatatt
    40621 tggcaacaac agaacgtcaa tctggaacag cttgctgatg gtgctgccaa aatggaccag
    40681 cgatccgata tccaccatca gtatcagccg ctgccagcac cggtcctgaa tcatccgggt
    40741 cagggtttcc agggtgtcat gcaccgactg ctcaaatggc atatcaattg cgtagaacag
    40801 atcgcgctcc agcacccggt tcacatattg cgcctggctc gttgcggtgg tcgccccgtg
    40861 agcgattaga atcacgccgc aatccgggct ggtgtcgatg cgctggcggt agtggcgaca
    40921 ctctttcagg aacaggcaca gccagaccac ctccgtcgcc gggcactgaa tatgcaacaa
    40981 ctcattgatt ttgcggcaca gaagcgtggc attgtcatac tcatctttac agcgatccag
    41041 aatcaggctg gatgaataga gttgaggtat aagccctcgc cgcacatagc caatcagcgc
    41101 aagaaagtgt ttacgtaacg ggttcgccag gttttctggc aatgtaaatc ctaatacctg
    41161 ctccacgtaa ccgatcagca gcgtcacacg ctcctcaatt tgatcgccat agcgcggtgg
    41221 atgcgccgca ctatggcggc tataaagacc atattcaaaa atcgaactga gtttgttttt
    41281 caggatagcc agcgtttctg cgggcggcac gttactgttg cgcagattga catattcacg
    41341 agtcaggaaa ctgtaaaaca ggtcgctctc ttctatttcc gcgctggtcg ccaacgaatt
    41401 ttttaacgcg ggcaacgtac gcgcatccac gttcagacga tctttaccgc caaacaacgc
    41461 atcgaccagc agacgctgct ctggcgtggt attaaacgac atctccgcca gtcgtttatc
    41521 taactctagc gtatcattac gctcggtcat tcccgacgcc cacgcctgcg cgcacaagaa
    41581 ctgaatatca cttttaagct ggccgatatt gccttccagg ggtttactta acaaccaatg
    41641 caagagcgtt ttatcaaggc ttatggtacg ttcaatttta cgactttcac gctgaaagaa
    41701 gcttacaatc aattcgagct gctcctcgac cgaacgctgg cgaatacccg gtaagtcaat
    41761 acatacctgg atacgccgct ggaaggtacg caatagtgcc gagttgaccg actctgtagt
    41821 ggcgcaaata aggcgcaccg aaattgaata tgttggccca cttgaaccaa gtgggcggta
    41881 ttcaccttta tcgagaatag aaaagagctt ctcttgtcct tcgtacggga ggcgatgcac
    41941 ttcatccaga agtaaataac ctccgtcagc ttgctccacc aatcccgttt tattctcact
    42001 tgccccggta aatgccccct tatggtgacc aaacagatgt gaggaaagca actccggatt
    42061 atgagcatat tcggcacagt taaagtaaac caacggaggt ggcgaaccta tggtgtgctt
    42121 gcaagcgaag caatgcatta actcagcaaa gaatgttttg ccaacaccag atgcaccggt
    42181 taacagcaca tgtagtccgt ggggataaag caccgctgcg cggccttttt ctaccgcatc
    42241 acggagacta cggtcgtatc cgataagtcc ggaaaacgga tcatcatctg tatatttttc
    42301 tttctttggc aataaactag ccactgaatg aactatgcat tcgcaatcat ccagttttcg
    42361 attaatgagc aattctagtg cgtgacgatg tagaaaaaaa acaggacgtc cctgactttt
    42421 taccaccagg ccttcattcc ataattgatt aagatcttta ctaaccgagt tgcgtgccag
    42481 cccaaggtta aaaccaatcg cctcagcagt aaatgctttc tccttcgcta aatcagacag
    42541 ttccagccca tacgtcagcc gctccagctc cttgtgaata acatcaatcc gtctcatttc
    42601 tttaccgctt aaaaaacaat agttgttcag aacatacact atttgtaaac gtaagaatga
    42661 gaactatcgg gaagaattaa tgttccttgt ggcaatctgg tttctgaata ttcgtgtttt
    42721 aaatattact tatggtctga aacaaccagg caggtgaaca gagctaagaa aaagtagcgt
    42781 agaaatataa aaacatatat cgcactgatg aatgtttccc ggcatgacta tgcacaccgg
    42841 gaaatattta gtaaattact cgcccttttt cgccgcctga atatacagca tctccagcgc
    42901 cagcgtcgcc gcagccagag cagtgatttc cgactgatca tatgccggag ccacttccac
    42961 tacgtccatc ccaacgatgt tgagatcttt caggccgcgt accagtttaa tagcgcgatc
    43021 ggaggtcagg ccgccaatca ctggcgtacc ggtgcctggt gcaaaagcag gatccaggca
    43081 gtcgatatca aaggtcaggt aaaccggcat atcacccaca atctgtttca cctgggcgat
    43141 aacgtcatcc acgctacgat cgttcacctg gcaggcgtcc agtacggtaa agccgttgtc
    43201 tttatcaaac tcggtacgaa taccaatctg cacggaatga ttcgggtcga tcagaccttc
    43261 tttcggcgcg gtatagaaca tggtgccgtg gtcaaattca caaccgttcg cgtaggtatc
    43321 ggtgtgggcg tcaaagtgta ccagcgccat tttaccgaaa tgcttcgcat gagcacgcag
    43381 cagcggcagc gtaacaaagt ggtcaccacc gaaggagagc atacgcttac cggcagccag
    43441 cagcttctcg gcgtgcgcct gcagcttctc gctcatctca cgggcatcgc cgaaagcata
    43501 taccagatca ccgcagtcca cgacgttcag acgctcacgc atgtcgaaat tccacgggaa
    43561 gcggttgtgt tcccaggcca gattcgtcga aacctgacgg atagctgccg gaccgtggcg
    43621 accacccgca cgaccagaag tggccatatc gaacggcacg ccagtaatca cccagtcagc
    43681 atcgctgtca tatggctgga agttcatcgg caggcgtaaa aaaccaaagg cgttggaaac
    43741 cagtgagtta tcgtattgat gacctaaggt gctcatggat ggactcctat tacaaagtcg
    43801 atataaaaaa acccttccgc gtcgttaggc ccgacgagga agggttggat ttgcgaaaca
    43861 caagtttgtg ctaattatcg ccttaaatgg gcgaggcatc aagcgaggtt gctatttccg
    43921 acacggtggc ggcgcatagc cattgttatc tgccggaaag gcttgtccag agcacggcgg
    43981 cagcgcgttt tgcgccgggc cttgtaattc aaaggcggca aagccgatta ccccggtatt
    44041 acgcacatcg ccttgcgccg aattggctgc gtaggaatca gcaacgttgg cgaactgaaa
    44101 agccgcttca gtatgtttat ctttgcggaa tcctttaatc gctaacgaat ctccggcatt
    44161 cacaatatag ccgttgttat tcagcgaacc ttgcttgccg ttcagcacat ccaggccatc
    44221 aaccgtagca acgatctcgt agttggtgtt acgactgtaa ttacgaacat ataattgata
    44281 attcattccg actctggcgt ggacaatata gcgtggttca ttattggatg aatcataaac
    44341 acgcgtaata ggtagagaat taaaattacc atcgcgaata gcatattcca gttcgttaat
    44401 acgaatgcta taaacgcggt cgtagccgga aggaacatta gtcgaataac taataacgat
    44461 catctccgcc ggatcgcgcc aggctcgctc agcagatacg ccctgaaccg ttgagtgcac
    44521 atccccaccc caggcggtgc cgacattacc cgatgcactg ctattatact gcgcattact
    44581 ggaacaaccg gccagcagca cgattaatgc caccaacgcg ctacgtactt tccatttttt
    44641 cataaccgtt ccctctgcat ttaattaatg cagcgattgt agggagatta tttaa
//
LOCUS       DAZFRN010000021        42434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFRN010000021 DAZFRN010000000
VERSION     DAZFRN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.021"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(208..855)
                     /gene="ychE"
                     /locus_tag="ACHKUB_001137"
     CDS             complement(208..855)
                     /gene="ychE"
                     /locus_tag="ACHKUB_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO6654182.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(886..939)
                     /gene="ychE"
                     /locus_tag="ACHKUB_001138"
     CDS             complement(886..939)
                     /gene="ychE"
                     /locus_tag="ACHKUB_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO6654183.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1332..4007
                     /gene="adhE"
                     /locus_tag="ACHKUB_001139"
                     /gene_synonym="adhC"
     CDS             1332..4007
                     /gene="adhE"
                     /locus_tag="ACHKUB_001139"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO6654184.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4131..5031
                     /locus_tag="ACHKUB_001140"
                     /pseudo
     CDS             4131..5031
                     /locus_tag="ACHKUB_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5313..5930)
                     /gene="tdk"
                     /locus_tag="ACHKUB_001141"
     CDS             complement(5313..5930)
                     /gene="tdk"
                     /locus_tag="ACHKUB_001141"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO6654185.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6536..6949
                     /gene="hns"
                     /locus_tag="ACHKUB_001142"
     CDS             6536..6949
                     /gene="hns"
                     /locus_tag="ACHKUB_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO6654186.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7093..8001)
                     /gene="galU"
                     /locus_tag="ACHKUB_001143"
     CDS             complement(7093..8001)
                     /gene="galU"
                     /locus_tag="ACHKUB_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO6654187.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8203..9216)
                     /gene="rssB"
                     /locus_tag="ACHKUB_001144"
     CDS             complement(8203..9216)
                     /gene="rssB"
                     /locus_tag="ACHKUB_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO6654188.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9308..10213)
                     /gene="rssA"
                     /locus_tag="ACHKUB_001145"
     CDS             complement(9308..10213)
                     /gene="rssA"
                     /locus_tag="ACHKUB_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO6654189.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10326..10784
                     /gene="ychJ"
                     /locus_tag="ACHKUB_001146"
     CDS             10326..10784
                     /gene="ychJ"
                     /locus_tag="ACHKUB_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO6654190.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10834..11676
                     /gene="purU"
                     /locus_tag="ACHKUB_001147"
     CDS             10834..11676
                     /gene="purU"
                     /locus_tag="ACHKUB_001147"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO6654191.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11836..11920
                     /locus_tag="ACHKUB_001148"
     tRNA            11836..11920
                     /locus_tag="ACHKUB_001148"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11870..11872,aa:Tyr,seq:gta)
     gene            11968..12099
                     /locus_tag="ACHKUB_001149"
     ncRNA           11968..12099
                     /ncRNA_class="other"
                     /locus_tag="ACHKUB_001149"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12283..12960)
                     /gene="narI"
                     /locus_tag="ACHKUB_001150"
     CDS             complement(12283..12960)
                     /gene="narI"
                     /locus_tag="ACHKUB_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6654192.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12960..13670)
                     /gene="narJ"
                     /locus_tag="ACHKUB_001151"
     CDS             complement(12960..13670)
                     /gene="narJ"
                     /locus_tag="ACHKUB_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6654193.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13667..15205)
                     /gene="narH"
                     /locus_tag="ACHKUB_001152"
     CDS             complement(13667..15205)
                     /gene="narH"
                     /locus_tag="ACHKUB_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6654194.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15202..18945)
                     /gene="narG"
                     /locus_tag="ACHKUB_001153"
     CDS             complement(15202..18945)
                     /gene="narG"
                     /locus_tag="ACHKUB_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO6654195.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(19338..20729)
                     /gene="narK"
                     /locus_tag="ACHKUB_001154"
     CDS             complement(19338..20729)
                     /gene="narK"
                     /locus_tag="ACHKUB_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO6654196.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            21068..22864
                     /gene="narX"
                     /locus_tag="ACHKUB_001155"
     CDS             21068..22864
                     /gene="narX"
                     /locus_tag="ACHKUB_001155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO6654197.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            22857..23507
                     /gene="narL"
                     /locus_tag="ACHKUB_001156"
     CDS             22857..23507
                     /gene="narL"
                     /locus_tag="ACHKUB_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO6654198.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(23508..24902)
                     /gene="ychO"
                     /locus_tag="ACHKUB_001157"
                     /gene_synonym="iatA"
     CDS             complement(23508..24902)
                     /gene="ychO"
                     /locus_tag="ACHKUB_001157"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO6654199.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDESLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            25087..25440
                     /gene="ychN"
                     /locus_tag="ACHKUB_001158"
     CDS             25087..25440
                     /gene="ychN"
                     /locus_tag="ACHKUB_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO6654200.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(25484..26179)
                     /gene="chaC"
                     /locus_tag="ACHKUB_001159"
     CDS             complement(25484..26179)
                     /gene="chaC"
                     /locus_tag="ACHKUB_001159"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO6654201.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     EPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(26337..26567)
                     /gene="chaB"
                     /locus_tag="ACHKUB_001160"
     CDS             complement(26337..26567)
                     /gene="chaB"
                     /locus_tag="ACHKUB_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO6654202.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26837..27937
                     /gene="chaA"
                     /locus_tag="ACHKUB_001161"
     CDS             26837..27937
                     /gene="chaA"
                     /locus_tag="ACHKUB_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO6654203.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            28342..28449
                     /locus_tag="ACHKUB_001162"
     CDS             28342..28449
                     /locus_tag="ACHKUB_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6654204.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            28878..28985
                     /locus_tag="ACHKUB_001163"
     CDS             28878..28985
                     /locus_tag="ACHKUB_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6654205.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            29413..29520
                     /locus_tag="ACHKUB_001164"
     CDS             29413..29520
                     /locus_tag="ACHKUB_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6654206.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(29669..30523)
                     /gene="kdsA"
                     /locus_tag="ACHKUB_001165"
     CDS             complement(29669..30523)
                     /gene="kdsA"
                     /locus_tag="ACHKUB_001165"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO6654207.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(30559..31368)
                     /gene="sirB1"
                     /locus_tag="ACHKUB_001166"
     CDS             complement(30559..31368)
                     /gene="sirB1"
                     /locus_tag="ACHKUB_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO6654208.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(31372..31764)
                     /gene="sirB2"
                     /locus_tag="ACHKUB_001167"
     CDS             complement(31372..31764)
                     /gene="sirB2"
                     /locus_tag="ACHKUB_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO6654209.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(31761..32594)
                     /gene="prmC"
                     /locus_tag="ACHKUB_001168"
     CDS             complement(31761..32594)
                     /gene="prmC"
                     /locus_tag="ACHKUB_001168"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6654210.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(32594..33676)
                     /gene="prfA"
                     /locus_tag="ACHKUB_001169"
     CDS             complement(32594..33676)
                     /gene="prfA"
                     /locus_tag="ACHKUB_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO6654211.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(33718..34974)
                     /gene="hemA"
                     /locus_tag="ACHKUB_001170"
     CDS             complement(33718..34974)
                     /gene="hemA"
                     /locus_tag="ACHKUB_001170"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO6654212.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            35188..35811
                     /gene="lolB"
                     /locus_tag="ACHKUB_001171"
     CDS             35188..35811
                     /gene="lolB"
                     /locus_tag="ACHKUB_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO6654213.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            35811..36662
                     /gene="ispE"
                     /locus_tag="ACHKUB_001172"
     CDS             35811..36662
                     /gene="ispE"
                     /locus_tag="ACHKUB_001172"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO6654214.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            36813..37760
                     /gene="prs"
                     /locus_tag="ACHKUB_001173"
     CDS             36813..37760
                     /gene="prs"
                     /locus_tag="ACHKUB_001173"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO6654215.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            37885..39564
                     /gene="dauA"
                     /locus_tag="ACHKUB_001174"
     CDS             37885..39564
                     /gene="dauA"
                     /locus_tag="ACHKUB_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO6654216.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(39619..39897)
                     /gene="ychH"
                     /locus_tag="ACHKUB_001175"
     CDS             complement(39619..39897)
                     /gene="ychH"
                     /locus_tag="ACHKUB_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO6654217.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            40175..40759
                     /gene="pth"
                     /locus_tag="ACHKUB_001176"
     CDS             40175..40759
                     /gene="pth"
                     /locus_tag="ACHKUB_001176"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO6654218.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSQVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            40876..41967
                     /gene="ychF"
                     /locus_tag="ACHKUB_001177"
     CDS             40876..41967
                     /gene="ychF"
                     /locus_tag="ACHKUB_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO6654219.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT        10607 a        10991 c        10385 g        10451 t
ORIGIN      
        1 caattattct tcagcaagcc caggattatg atgtggcgaa aataacatga tggtgaggag
       61 aagcagttaa ggaaatcctt ataactaact gtaatttgag aagaatattt ttacctattc
      121 cttttgcttt tgataactgc gcgggaacaa agtaaaaata atgacgaagt aaacggagca
      181 tggcagctcc gtttcatttg aaaggaatta attaagcagg ccggggaaaa tccccttaat
      241 accagtaacg ataaattcaa tccccaatgc catcagcaat agccccataa tacgcgtaat
      301 cacgttgatg ccggtctggc gtaaaacccg taccagccac ggtgccatgc ggaacaatcc
      361 ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa ccaaacagat agctaatgct
      421 gtgataacgc gtaccccaga cgatggtaga actgatcgcc cctggccccg ccatcaacgg
      481 caacgccaat ggcaccacac caatgctttc acgtaccgcg gtttctgatt tttcttgctt
      541 gttctgttta tcctcgccaa gcttgccgct gatcatcgac atcgctatcg tcaccaccag
      601 gatgcccccg gcgatacgga acgaatcaat tgatatacca aaaagttgca gaatcgtgtc
      661 gccgagaaaa agcgagatcc acaagataat ggccacagac aggttggctg taaggttagt
      721 tttgtttcgc gctgccgctg tctgataact ggtcatgctg ataaagacgg gaataatccc
      781 taccgggttg accagcgcaa ataacccgat gaaaaatttg aagtaaacgg gaaaatcaaa
      841 aaaggtctga atcacggtta gctccgaagc aaaagccgga taatgttagc cataaataag
      901 gttgaaaaga cgcgctgaca atacgccttt tgacagcatt tttcacctcc taactactta
      961 aaattgctat cattcgttat tgttatctag ttgtgcaaaa catgctaatg tagccaccaa
     1021 atcatactac aatttattaa ctgttagcta taatggcgaa aagcgatgct gaaaggtgtc
     1081 agctttgcaa aaatttgatt tggatcacgt aatcagtacc cagaagtgag taatcttgct
     1141 tacgccacct ggaagtgacg cattagagat aataactcta atgtttaaac tcttttagta
     1201 aatcacagtg agtgtgagcg cgagtaagct tttgattttc ataggttaag caaatcatca
     1261 ccgcactgac tatactctcg tattcgagca gatgatttac taaaaaagtt taacattatc
     1321 aggagagcat tatggctgtt actaatgtcg ctgaacttaa cgcactcgta gagcgtgtaa
     1381 aaaaagccca gcgtgaatat gccagtttca ctcaagagca agtagacaaa atcttccgcg
     1441 ccgccgctct ggctgctgca gatgctcgaa tcccactcgc gaaaatggcc gttgccgaat
     1501 ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca ctttgcttct gaatatatct
     1561 acaacgccta taaagatgaa aaaacctgtg gtgttctgtc tgaagacgac acttttggta
     1621 ccatcactat cgctgaacca atcggtatta tttgcggtat cgttccgacc actaacccga
     1681 cttcaactgc tatcttcaaa tcgctgatca gtctgaagac ccgtaacgcc attatcttct
     1741 ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc tgatatcgtt ctgcaggctg
     1801 ctatcgctgc cggtgctccg aaagatctga tcggctggat cgatcaacct tctgttgaac
     1861 tgtctaacgc actgatgcac cacccagaca tcaacctgat cctcgcgact ggtggtccgg
     1921 gcatggttaa agccgcatac agctccggta aaccagctat cggtgtaggc gcgggcaaca
     1981 ctccagttgt tatcgatgaa actgctgata tcaaacgtgc agttgcatct gtactgatgt
     2041 ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca gtctgttgtt gttgttgact
     2101 ctgtttatga cgctgtacgt gaacgttttg caacccacgg cggctatctg ttgcagggta
     2161 aagagctgaa agctgttcag gatgttatcc tgaaaaacgg tgcgctgaac gcggctatcg
     2221 ttggtcagcc agcctataaa attgctgaac tggcaggctt ctctgtacca gaaaacacca
     2281 agattctgat cggtgaagtg accgttgttg atgaaagcga accgttcgca catgaaaaac
     2341 tgtccccgac tctggcaatg taccgcgcta aagatttcga agacgcggta gaaaaagcag
     2401 agaaactggt tgctatgggc ggtatcggtc atacctcttg cctgtacact gaccaggata
     2461 accaaccggc tcgcgtttct tacttcggtc agaaaatgaa aacggctcgt atcctgatta
     2521 acaccccagc gtctcagggt ggtatcggtg acctgtataa cttcaaactc gcaccttccc
     2581 tgactctggg ttgtggttct tggggtggta actccatctc tgaaaacgtt ggtccgaaac
     2641 acctgatcaa caagaaaacc gttgctaagc gagctgaaaa catgttgtgg cacaaacttc
     2701 cgaaatctat ctacttccgc cgtggctccc tgccaatcgc gctggatgaa gtgattactg
     2761 atggccacaa acgtgcgctc atcgtgactg accgcttcct gttcaacaat ggttatgctg
     2821 atcagatcac ttccgtactg aaagcagcag gcgttgaaac tgaagtcttc ttcgaagtag
     2881 aagcggaccc gaccctgagc atcgttcgta aaggtgcaga actggcaaac tccttcaaac
     2941 cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga cgccgcgaag atcatgtggg
     3001 ttatgtacga acatccggaa actcacttcg aagagctggc gctgcgcttt atggatatcc
     3061 gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc gaaaatgatc gctgtcacca
     3121 ccacttctgg tacaggttct gaagtcactc cgtttgcggt tgtaactgac gacgctactg
     3181 gtcagaaata tccgctggca gactatgcgc tgactccgga tatggcgatt gtcgacgcca
     3241 acctggttat ggacatgccg aagtccctgt gtgctttcgg tggtctggac gcagtaactc
     3301 acgccatgga agcttatgtt tctgtactgg catctgagtt ctctgatggt caggctctgc
     3361 aggcactgaa actgctgaaa gaatatctgc cagcgtccta ccacgaaggg tctaaaaatc
     3421 cggtagcgcg tgaacgtgtt cacagtgcag cgactatcgc gggtatcgcg tttgcgaacg
     3481 ccttcctggg tgtatgtcac tcaatggcgc acaaactggg ttcccagttc catattccgc
     3541 acggtctggc aaacgccctg ctgatttgta acgttattcg ctacaatgcg aacgacaacc
     3601 cgaccaagca gactgcattc agccagtatg accgtccgca ggctcgccgt cgttatgctg
     3661 aaattgccga ccacctgggt ctgagcgcac cgggcgaccg tactgctgct aagatcgaga
     3721 aactgctggc atggctggaa acgctgaaag ctgaactggg tattccgaaa tctatccgtg
     3781 aagctggcgt tcaggaagca gacttcctgg cgaacgtgga taaactgtct gaagatgcgt
     3841 tcgatgacca gtgcaccggc gctaacccgc gttacccgct gatctccgag ctgaaacaga
     3901 tcctgctgga tacctactac ggtcgtgatt atgtagaagg tgaaactgca gcgaaaaaag
     3961 aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc cgcttaatca gtagcgctgt
     4021 ctggcaatat aaacggcccc ttctgggcaa tgccgatcag ttaaggatta gttgaccgat
     4081 ccttaaactg aggcactata acggcttcca caacagggag ccgttttctt atgccacttc
     4141 tcaatgatct gctcgatttc agtgaccatc cgcttatgcc tccgccctct gcacaactat
     4201 ttgcagaaca ccttcccacc gagtggatac aacactgcct gacgctttct gctcatgcga
     4261 ccgttcgccg ccgtcgttta ccgggggaca tggttatctg gatggtggtg caatgagcca
     4321 attaccgatg ttgttcgccg tctgaacctg agcgcggatg gcgaagcggg gatgaacctg
     4381 ctggcccgca gcgctgtcac ccaggcgcgt cagcgcgtgg gggccgcccc agtggaatgg
     4441 ctcttccgcc agaccgcaca gacaggggcg cggaacgtta cctgaaggat gactggcacg
     4501 gcctgcaact ttttgccatt gatggcgcac agttcaggac acctgactga taaacccgag
     4561 ctgcgtgaat attatgggtc tgccaacaca tccactaagc ggcagaacgc ctacccggtt
     4621 atgcgtctgg tggccttaat gaacctggga agtcacattc tgctgaatgc cgtgaccgca
     4681 ccttaccggc agagcgaaac tgtgctggcc cactccatgc tcgccaccat cccggataac
     4741 tctattacgt tgtttgataa acttttttac agcgaagacc tgctgctgac gctgaaccag
     4801 aagggttgta accgccactg gctactgcct gcgtggaaga atatcgcatc agaaatgata
     4861 gagctgggaa acacggcgtc accagggacc atcccgaaac gactggagca tcttcagggt
     4921 gctctggaag tggtgttcat aacaaaacgc ccccgaccat caaggccgag gtcggttaag
     4981 atatcaaaaa cccgttatcc tgtgaaacat agtgccgctc ctcttaagtg aacggcattg
     5041 cccttctggg gccgtatttt ttgtttaccc aaagcaactt ttccataaac cgacagcatt
     5101 agccttcatc atatttgcga cgatgtataa cgcctaaaca cagggatatt gtactttaca
     5161 ggtcacaagt caacgtcggt gcttaagagc cctgtgaggc gtatagcggc gttaaaaaac
     5221 tgccgagaag ggtatatagc ccggaagaag tgcgtaaaac gaactgacag gataaaagtg
     5281 ccctgctcac cctgtcagta aagaaattct tattaatcgt ggcgatgcct ttcctgaata
     5341 gccgttaatg agccgacttg taacgcctct ttatagtgtt tacggcatac agaaacgtat
     5401 cgttcattac caccaattac cacctgctca ccttcgttat aaggtctgcc tgcttgatca
     5461 agacgcagca ccatgcttgc tttacggcca caaaaacaga tggtttttaa ttcaaccagt
     5521 ttgtcggacc atgccagtaa gtattggctg ccaataaata attcacctcg aaaatcggta
     5581 cgtaaaccat aacaaagtac gggtatatcg agttgatcga caacctccga taattcatat
     5641 acttgttgtc tggttaaaaa ctggcattca tcaaccagta cgcaatgaat tgcctgctgt
     5701 tcatgttccg cacgaatctc atcaaataat gatgaatttt ggttaaataa ttttgcaggc
     5761 gatgacaaac ctatacgcga actgactttc ccggcaccaa agcgatcatc aatttctgcc
     5821 gtatatacga cagtgcgcat gccgcgttcc tggtaattgt atgaagattg caacaatgct
     5881 gtagacttac ccgcattcat tgcggaatag tagaaatata gctgtgccat cagccacagg
     5941 ccctcaatga tatgaaataa agttggctgg agtttatcat aattcgtcag tttttcagct
     6001 caacagtatg cacagaagtg ggtatatgcg ttctccctta cgaagccttg cataatcctt
     6061 ctgagctatc attacaactg cctcgcttgt tataagcggg taaatgactg ctggtaacta
     6121 ttcacaatct ttaacctgtt gcgcaagtaa tagccctctg ttgacctcca ggagatagtg
     6181 caatactaag tccatgctct tattgcgact gttctacttt tcatcattcg cttaataggg
     6241 aattctcgta aacacaacta atacagaaga ctgaaaggtc gtcagcctac gataatctcc
     6301 ccataaaatg tgacatgaat caggaagttt taacctcacg tgctgcgaaa tcatcggtgt
     6361 aaatagggct atatgccgcg tcttttctgg ctaattttat gaaaagatat ttattggcgg
     6421 cacaaaataa agaacaattt tgaattcctt acattcctgg ctattgcaca actgaattta
     6481 aggctctatt attacctcaa caaaccaccc ccaatataag tttgagatta ctacaatgag
     6541 cgaagcactt aaaattctga acaacatccg tactcttcgt gcgcaggcaa gagaatgtac
     6601 acttgaaacg ctggaagaaa tgctggaaaa attagaagtt gttgttaacg aacgtcgcga
     6661 agaagaaagc gcggctgctg ctgaagttga agagcgcact cgtaagctgc agcaatatcg
     6721 cgaaatgctg atcgctgacg gtattgaccc gaacgaactg ctgaatagcc ttgctgccgt
     6781 taaatctggc accaaagcta aacgtgctca gcgtccggca aaatatagct acgttgacga
     6841 aaacggcgaa actaaaacct ggactggcca aggccgtact ccagctgtaa tcaaaaaagc
     6901 aatggatgag caaggtaaat ccctcgacga tttcctgatc aagcaataat cttttgtaga
     6961 ttgcacttgc ttaaaatccc gccagcggcg ggatttttta ttgtccggtt taagacaatt
     7021 taataagaat aagctataaa aaaacggcgt cgattgctca acgccgtttc gtggataaca
     7081 ccgatacgga tgttacttct taatgcccat ctcttcttca agccaggctt taaattccgt
     7141 gccaagggtg ttatgacgaa taccgtattc aacgaaggcc tgcatgtaac ctaatttatt
     7201 accgcagtca tggctcttcc ctttcatatg ataggcttcc accgtttctt tttcgatcag
     7261 catatcaatt gcgtcggtga gctgaatttc atcaccagct cccggagggg tttttgccag
     7321 caacggccaa atatccgcgc taagtacgta acgacccaca atagcgagat tagacggcgc
     7381 aacatccgct ttcggttttt ctaccacacc aaccatcggt acgctttcac ccggcgctaa
     7441 ttcaacgcct ttgcaatcca caacgccata tgcggtcaca tcagcaaccg gttcaaccat
     7501 gatctggcta tgacccgttt catcaaagcg gcggatcatc tctgccaggt tatcctgtga
     7561 caaatcggat tcatattcat ccagaataac atcaggcaaa ataacagcta ccggttcatc
     7621 acccactacc gggtgagcac acaataccgc gtgtcccagg cctttcgcca gaccctgacg
     7681 aacttgcata atagtcacgt gcggtggaca aatagactgc acttcatcaa gcagttgacg
     7741 ttttacacgt ttttccagca ttgcttccag ttcaaaactg gtatcaaagt ggttttcaat
     7801 agagttttta gatgagtgtg taaccagcac aatttcagta atgccagccg caatacattc
     7861 attcacgacg tattgaatta atggcttatc gacaagtggc agcatctctt tcgggatggc
     7921 tttcgtcgcc ggcaacatcc tggttcctaa tcccgcaacg gggataacgg cttttttgac
     7981 tttcgtatta atggcagcca tttaaattct cctggactgt tcgtgttttg aacgtgttca
     8041 tatttctgta tcgcatccca gtatatcagc accccgaagg ggctaagttc tgaaaaggag
     8101 gcgagttagc ataattaaga caattactta tacgaatggc gacgatagta ccaccagccg
     8161 acattagcag gtaatgcaaa tttagcccgc gttatcgttt gctcattctg cagacaacat
     8221 caagcgcagt cgaccaccgg ttccccatat ttggcattgc caggcatcgc atcgctggct
     8281 caattgattc aaataagcgt tacctaaagt gcctaacgga acaccattac tgatttgcac
     8341 ctggtgttcg ccggtattta acgtcgcatt cagacccgca gaaaccagaa tgagattttt
     8401 cagttcgcga tgataatagc caactaatag cggaaactgc cccggcagat tggcctgacg
     8461 aagtaaatgg tttacctgct tcaataacgc gcccaactct ggcaaccgtt gattttggtg
     8521 tgcaagctgt tcctgtaata atccgttaaa caatgcgcgt aataacaagg cagcaagtac
     8581 gccattatgt ccagctcggg tgacatcaag gcaataaaat gccagatcgt tttccgaaag
     8641 tgcggcaata tcaagcacca ggccgggttt gtccgcggca accaattgac gataattaac
     8701 ccggcaatgg gaaatcacct gctgaaccgg cggttgtagt tcctgtaata atttcgccgc
     8761 tgcggcaggg ttatcaacca ttgcatccca gtcgcgaaaa agcctttcct cttcctcaac
     8821 gcgcgaatta aacatgctgg gatagagaca ggcaaaaacc atctcgcgca agcgattcag
     8881 atctttaact ggtttcagca aaacatcttc aacgcccaga cgtaacgctt tggcaatatc
     8941 tgccatattt tcagtggcag atatcaccag aactggggtc tggtcgcctc tgttacgtat
     9001 atgctccagc agtttaagcc cgttcattcg tggcatcgcg atatcacata tcatcaggtc
     9061 tggagtgaaa cctcccagca actcaagggc atccacccca tcagccgcca gtaccgttgt
     9121 cgctcccaat gaggaaaacc atgaatccag aagcgagcga aatacctgct catcttcaac
     9181 aatgagaatc tgttttccga ccaatggctg cgtcatgttc tctcccctga ctggctttac
     9241 tcaatagtgg catgctattg ccacctgcgc ctgtcagaat taacttaagt gtagataaaa
     9301 attctggtca aatgttggtg cgtaccaacg gcaaaagttc gtccattttc ctttccactg
     9361 ccagctgtcc ggccgcaatg gcagcgtgcg cgcgatggaa atcaagcgta gatatttgcg
     9421 ggcaaacagg ttgaatcaga atatcgggcg gatcacctgc catgcggttc cttttaaggc
     9481 ggttctccag cacctggata gaagtggtca taatctctgt tgccgttggc gctgtcaccg
     9541 cccgacgtgt cgttatgctg cctaaccttt ctttcagacg cgcatgccac ggcagagaat
     9601 caccattctc gctattttct tcactgacat taaaggagag caaatcttgt tgcatcaaat
     9661 gagcatcgtg ctgcaggtca accgctatca caatatcagc ccccaatgca cgcgtgaggg
     9721 aaataggaat tgggttaacg actgctccat caaccagcca gtagccgtta tgtgcaacag
     9781 gtgccatgag tcctggaata ctgcatgatg cgcgaatagc aagatggaga tcgccttcag
     9841 taaaccataa ttcacgtccc gtacttaaat tggtggcaac agccgcaaag cgacgggaac
     9901 aattttcgat ctctgtttcc ggcattattt cgcggtattg attgaagaca cgctcgccgc
     9961 gcagtaaccc accgcgctgc caggagagat ccatcaggcg taaaacatcc caataactga
    10021 aagaggtcac ccaatcttcc agcgcagata atcgatcgca tgcataggca gcgcccacca
    10081 gcgaaccaat tgaacatcct gcaacgatat caatttcaat acccactttt tttagcgcat
    10141 taataacgcc aatatgcgac caacctctcg ccgcgccaga tcccagcgcc agccctattt
    10201 ttatctttct cattattccc gcaagattcc cctggaatgc aatcgtagcc acatcgctac
    10261 tgctcggtta acatagtgct actcaggcgg ttaatacgcc gttattgttt ccagggagat
    10321 tatttgtgtc tcagctttgt ccctgtggta gtgctgtcga gtatagccta tgttgccacc
    10381 cttatgtgtc tggtgaaaag gttgcacctg atccagaaca tctcatgcgt tcgcgttact
    10441 gcgcttttgt gatgcaagac gcagattatt taataaagac ctggcatccc tcttgtggag
    10501 cagcggcgtt acgtgccgaa ttgatggccg gatttgcgca tactgaatgg ttgggattga
    10561 ccgtatttga acattgttgg caggacgccg acaatatcgg atttgtcagt tttgttgctc
    10621 gatttaccga aggtggtaaa acaggtgcaa taattgaacg ttcgcgattt ttgaaagaaa
    10681 acggtcaatg gtactatatt gacggtacac gtccgcagtt tggtcgcaac gatccctgcc
    10741 cttgtggttc aggtaaaaaa tttaaaaagt gctgcggcca ataatggttg acggtacggt
    10801 ttagcaaaca ctctcaacaa ggtttttcca gcaatgcatt cactccaacg taaagttctg
    10861 cgtactattt gtccggacca aaaaggtctg atcgcacgta ttaccaatat ttgctacaag
    10921 cacgagttaa atatcgtaca gaacaatgaa tttgttgatc accgtaccgg gcgctttttt
    10981 atgcgcacgg aactggaagg gatttttaat gattccaccc tgctggcgga tctcgatagc
    11041 gcattgccag aaggctccgt gcgtgagctg aatcctgccg gtcgtcgccg gatagtgatt
    11101 ctggtcacta aagaagcgca ttgccttggc gatttgttga tgaaagccaa ttacggcggc
    11161 ctggatgtcg aaatcgcggc agttattggt aaccacgata ctttacgttc tctggttgag
    11221 cgttttgata ttccgtttga gctggtaagc catgaagggt taagccgcaa cgagcacgat
    11281 caaaagatgg cggacgccat tgatgcttat caacctgact atgtggtgct ggcgaagtat
    11341 atgcgggtat taacgccgga atttgtggca cgcttcccga ataaaatcat caatattcac
    11401 cattcattcc tgcctgcgtt tatcggcgca cgtccttatc accaggccta tgaacgtggt
    11461 gtgaagatta ttggcgcaac cgctcactat gtgaatgaca atctggacga aggcccaatc
    11521 atcatgcagg acgttattca tgtcgatcat acctacacag ctgaagatat gatgcgcgca
    11581 ggtcgtgacg tcgagaaaaa cgtcttaagt cgtgcgctat acaaagtact ggcacagcgc
    11641 gtctttgttt acggtaatcg gacgattatt ctttaatcgc cagcaaaaat aactggttac
    11701 ctttaatccg ttacggatga aaattacgca accagttcat ttttctcaac gtaacacttt
    11761 acagcggcgc gtcatttgat atgatgcgcc ccgcttcccg ataagggagc aggccagtaa
    11821 aaagcattac cccgtggtgg ggttcccgag cggccaaagg gagcagactg taaatctgcc
    11881 gtcatcgact tcgaaggttc gaatccttcc cccaccacca tcacttttca aaagtccgta
    11941 aactctcaaa cgaatccgca attaaatatt ctgcccaagc ggggaaggat gagaagcttc
    12001 gaccaaggtt cgacttgagc gccagcgaga gagcgttgcc gcaggcaacg acccgaaggg
    12061 cgaagcgcgc agcgctgagt aatccttccc ccaccaccat cgcttcctta aataatcaaa
    12121 acctgttact ccgagctaat tttgcccgat ggcaccacgt ttaccgggcc tacaatatcc
    12181 tcccctgtag gtcggataag cgaagcgcat ccgacaactc accccgcacc gtgcccataa
    12241 aaaaaccccg ccgaagcagg gtctatgtga actaaaattc gcttagtgac gagcgcgcac
    12301 cagctggtac ttacgtgtca gatactccac cggtacgctc cagatgtgta ccagacgcga
    12361 gaacgggaac agcaggaata acgtcatccc cagcaccagg tgcagacgga agataaacgc
    12421 cacaccatcg aggtgttgag aagcgccacc gtggaaggtc accaccgact gcgcccagcc
    12481 aaccagtttc atcatctcgc taccgtccat atgctgagcg gagaacggaa tggtcaacag
    12541 gcccagcgcg cactggataa cgagcagcga caggatcagg atatccgctc cggtagtggt
    12601 tgcacgcacg cgtgggctga acagacgacg cttcagcaac agcacgccgc caatcagaca
    12661 cagcacgccg ctggcaccac cagcaaacat cgccattttc tgtttcactt caatcggcag
    12721 ccaggcttca tacatccagt gcggcgtcag cataccgaag aagtgaccaa caaaaatccc
    12781 cagaataccg atatggaaca ggttcgacgc caggttcatc cctttgcgat ccagcatctg
    12841 gctggacgcc gcgcgccagg tgtactgccc gtagtcataa cgcagccagc taccaatcag
    12901 gaagaccgcc ccggctatgt acgggtagat atcaaagaag aacatattca ggaattgcat
    12961 tagtgctgtc ctccggtggt gatattcaga tattgcggcg cgacggcacc ggcaaagcga
    13021 cgctgatgag cagtgattgc tgaatcaccg cagcctttgt cagcaaagaa tttaacctgc
    13081 tcttcttccc agacagcatc cagcgcctgc ggcgtatcat cgcgcgcttc gtcggcaatt
    13141 ttttccgcca ctttgtcgct gtcgatagcg gtattggcca atttcagcag cagatcaaac
    13201 atcacggcat aacggctttc acgctgttgc agacgcgcgc tcagcaatgc cagaatcggc
    13261 gcgatatctt tcaaaccttc cacggcttcg ctttgcggca gctgcgacag gtactccaga
    13321 tacagcggca gatggtccgg taattcgcgg ctgtttaact gcaagccgtg ctgctcgtac
    13381 tgcgccagca ggtccaccat cgcctggccg cggtcacggg attcaccgtg aacatgttca
    13441 aatagcaaca gtgacgtggc gcggccacgg tcgaacagtt cgctgtactg ggcctgggca
    13501 tcgagcggat ccatcgccgt taaatcgcgc aggaaaatgc ccagcgcatg ggcatcttct
    13561 ttcgacagat ttttcgacgc agcaatcgcc tcaaacatct cttgttgatg ctgccataag
    13621 gcagcatccg gatattcaag gagacgcgat acaatcacga gttcgatcat ggatgcggct
    13681 ccgttttgct ggtcacatcg atggcatcga tacgacggct gttgaacaga ttgaatttgg
    13741 tatctgaacc gtggcagcca tcaccaaagg taaagccgca gccatttttc tccgggaagg
    13801 cttcccgtgc cagttcacga tgactactcg gcaccacaaa gcgatcttcg tagttagcaa
    13861 tcgccagata acggtacatc tcctgtgcct gggcttcggt cagaccgacc tcttccagcg
    13921 cacgggtatc aactttaccg tcaacggttt cagcacgttt gtaatgacgc atcgccagca
    13981 tacgtttcag tgcgcgcagt accggtttgg tatcaccggc ggtcagcaga ttcgccagat
    14041 actgtaccgg aatacgcaag ctttcgacgt ctggcagaat gccgttgcta cccagctcac
    14101 ccgcgtctgc tgcagactga atcggagaca gaggcggcac gtaccagacc atcggcagtg
    14161 tgcgatattc tggatgcagc ggcagcgcca gtttccattc cattgccatt ttataaaccg
    14221 gcgactgctg tgcggcttca ataacgctca gtggaatacc gtctttaatc gcctgctcga
    14281 tgactttcgg atcgttcgga tcgaggaaca cctccagctg acgctggtaa agatctttct
    14341 cgttctcggt gctggctgca cgttcaatcg cgtcggcatc gtacagcagc acgccaagat
    14401 aacggatacg accgacacag gtttctgagc acacggtcgg ctgacccgct tcaatacgcg
    14461 gatagcagaa gatgcacttc tcagacttac cgctcttcca gttgaagtag atttttttgt
    14521 acgggcatcc ggtgatgcac atacgccagc cacggcattt atcctggtcg atcaggacga
    14581 tgccatcttc ttcacgcttg taaatcgcac cgctcgggca ggtcgccaca catgccgggt
    14641 tcaggcagtg ttcgcacagg cgcggcaaat acatcataaa ggtgttttcg aactggctat
    14701 acatcgcctt ctggatgttg tcgaagttct tgtctttcgc cagtttgtca aactcaccac
    14761 ccaggtcatc ttcccagttc ggcccttttt cgattttcgc catccgttcc ccggtaatca
    14821 gcgaacgcgg acgggcaatc ggctgcgatt tgctgccttc cggcgcggta tgcaggttct
    14881 gatagtcaaa atcgaacggc tcgtaataat cgtcgatccc cggcagatgc gggttagcga
    14941 agattttacc cagcaacatg gcacggttac ccatgcgcgg ctgcagtttg ccgttgattt
    15001 tacggatcca gccgcctttg tatttttcct ggttttccca gtcagtcggg aagccctgac
    15061 ccggcttggt ttccacgttg ttgaaccacg cgtattccac gccttcacgg ctggtccaga
    15121 cgtttttaca ggtaactgaa caggtgtggc agccgatgca cttatcgaga ttcagcacca
    15181 tgccgacttg tgaacgaatt ttcattttac gctctcctgt acctggtcat tgccttcgcc
    15241 atctaaccag tcaatgttct tcatcttacg cactacaaca aactcatcgc ggttagaacc
    15301 tacggtgccg tagtagttaa agccgtatgc cagatgggca tagccgccga tcatatgcgt
    15361 cggtttcggc gtgatacggg tgaccgagtt atggatacca ccacgctgtt gggtaatttc
    15421 cgaaccaggc aggttaacga tacgttcctg cgcgtggtac atcatggtca tccctgccgg
    15481 aacacgctgg ctgacaaccg cacgggcagt cagagcaccg ttgctgttga agacttcaat
    15541 ccagtcgtta tcggcgatac ccagatcttt ggcatcggct tcactcaacc agaccaccgg
    15601 accaccgcga cctaaagtca gcatcagcag gttgtcgctg taggtggagt ggatacccca
    15661 cttctggtgc ggcgtcagga agttgagcgc tttttccggg ttgccattgg atttctggcc
    15721 catcacttct ttcaccgaac gggtgtcgat cggcggacga taaaccagca ggctttcacc
    15781 gaaatcacgc atccattggt gatcctgata cagctgctga cggccagaga gcgtacgcca
    15841 cgggatcagc tcgtgaacgt tggtgtaacc ggcgttgtaa gaaacgtgtt catcttccag
    15901 accagaccag gtcgggctgg agatgatttt gcgcggctgt gcctgaatat cgcggaagcg
    15961 aatcttctcg tcttctttat tcagcgccag atgcgtatgg tcacgaccgg taaactcgct
    16021 cagggcagcc caggctttca cggctacctg accgttggtt tccggtgcca gcgtcaggat
    16081 catctctgcc gcatcaattg cggtgttcag catcggctgg cctttcgccg gaccttccgc
    16141 tttggtgtag ttgagcttac gcagcagatc catctcgctc tgggtgttcc aggcaatccc
    16201 tttaccgcca ttaccgattt tctccatcag cgggccgata gaggtaaagc gttcgtaagt
    16261 cgccggataa tcgcgctcta cgaccataat gtgtggcgcg gttttaccag ggatcaggtc
    16321 gcactcgcct tttttccagt ctttcacatc cagcggctgc gccagttcag cggcagagtc
    16381 gtgctggata ggcagcgtga cgatgtcggt ttctttaccc agatgaccga cgcacacttc
    16441 ggagaatttc ttcgcgatgg ctttgtaaat ttcccagtcg cttttcgctt cccaggccgg
    16501 atcgaccgcc gcagacagcg ggtgaataaa cggatgcata tccgaagtat tcatgtcgtc
    16561 tttttcgtac caggtcgccg tcggcaaaat gatgtcggaa tagagacagg tgctcgacag
    16621 acggaagtcc agcgtaacca ccagatccag cttgccttcc agaccattgt cctgccagtc
    16681 cacttcttcc ggcttcacgc cgccctgttg ccccagatct ttaccctgga taccgtgctc
    16741 cgtccccagc aggtacttga gcataaactc atgaccttta ccggaagaac cgagcaggtt
    16801 agaacgccag atgaacaggt tgcgcgggtg gtttttaccg ttttctggtt gttctgccgc
    16861 aaaacggatg gaaccctctt taagggattt cacggtgtaa tctaccggat tcatcccggc
    16921 tttttctgct tcgccagcga tagtcagcgg gttagtgcct aactgcggcg cagacggcag
    16981 ccagcccatg cgttctgcac ggacgttaaa gtcgatcagg tgtccggtat aacgggattt
    17041 atccgccatc ggcgacagca gttcttccgc agtcacggtt tcataacgcc actggctgga
    17101 gtgattatag aaataagaag tgctgttcat atgacgcgcc ggacgctgcc agtcaagggc
    17161 aaacgccagc ggctgccagc cggtttgcgg acgcagtttt tcctgaccta catagtgcgc
    17221 ccagccgccc ccgctctgac cgacacagcc gcagaaaatc agcatgttga tcagaccgcg
    17281 atagttcata tcgaggtgat accagtggtt cagccccgca ccgacgataa tcatcgaacg
    17341 accgtgcgtt ttatcagcgt tatcggcaaa ttcacgggcg atgcgaataa tctggctgcg
    17401 agaaacgccg gtgatctgct cagcccaggc cggggtgtaa gctttcacat cgtcatagct
    17461 ggttgcacag ttaacgtcgt tcaggccacg ttccagaccg tagtttgcca gcgtcagatc
    17521 ataaacggtg gtcaccaggg cggtgctgcc atcagccagt tgcaggcgtt tcaccggcag
    17581 tttgtgcagc agcacgtttt ccagttccac tttgttgaag tgttccgtgc cgtcgccacc
    17641 aaagtacggg aagcctacct cagcgatctc atcctggcta cccagcaggc tcagttgcag
    17701 ctcggtttct tcgccagttt tgccgtcgcg ctgctcaaga ttccatttgc ccttctcgcc
    17761 ccagcggaag ccaatagaac cgttcggcgc aaccatttcg ccattggtat taaaggcgac
    17821 agttttccat tccggattgt tttcctggcc cagcgcatca accagatcag cagcgcgcag
    17881 catacgacct gcagcgtagt aaccgtcgcg ttcttccagc atcaccagca tcggcatgtc
    17941 ggtgtagcga cgcacatagt cggtgaaata ctggcttggg ttgtcgaggt ggaattcacg
    18001 cagcattacg tggcccatcg ccagcgccat tgccgcatcg gtgccctgtt tcggtgccag
    18061 ccacagatcg cacagtttgg cgatttcagc gtagtctggt gtgacggcaa cagttttggt
    18121 ccctttgtaa cgcacttcag taaagaagtg agcatccggg gtacgcgtct gcggtacgtt
    18181 tgacccccag gcgatgatgt agctggagtt gtaccagtca gcagattccg gtacgtcagt
    18241 ttgctcgccc caggtttgcg gagacgcagg aggcaagtcg cagtaccagt cgtagaagct
    18301 taagcaagta ccgccaatca gcgagagata gcgtgcaccc gatgcgtaag aaaccatcga
    18361 cattgccgga attggcgaga aaccagcaac acggtccggg ccgtagtttt tgatggtgta
    18421 aacgttagat gcggcgatca gttcgttcac ctcctgccag gaagaacgaa caaatccacc
    18481 gcgtccacgc gcctgcttaa agcttttcgc tttatcggcg tcttcaatga tagaagccca
    18541 tgcctcaacc ggatcgctat gcagcgcctt cgcttcacgc cacattttca tcaggcgttt
    18601 gcgcatcatc gggtatttca ggcggttggc gctgtaaaga taccaggagt agctggcacc
    18661 gcgcgggcag ccgcgaggtt catggtttgg cagatccgga cgggtacgcg gatagtcagt
    18721 ctgctgggtt tcccaggtga ccagaccgtt tttgacgtag attttccagc tgcaggagcc
    18781 ggtgcagttt accccgtggg tagagcggac gattttgtca tgctgccaac gctggcgata
    18841 tccatcctcc cagtcacggt tggtattgag aagctggcca tgcccatcgg caaaggtttc
    18901 acccttctgt ttgaagtagc gaaaccggtc caggaattta ctcatcggtt ttctcctgtg
    18961 ggagcctgtc ggccctctga taaatcgaca ttgctaatgt ttaagggcaa ggtaacgctc
    19021 tgaaacagcg tgggaattga taacgatcaa gagtgatggg gatgaaaaat aaagtaaatt
    19081 cctaagatac ccctttagga taattaacag ccaccacata acagactgta aattaagacg
    19141 ttttatacct gtagttattt aaactattaa gatttttatg cagatgggta ttaaggagta
    19201 ttccccatgg gtaacatatt aatggccctt aatgaaatcc ggcgaaataa gccatataat
    19261 atgagtatgt tgtaactaaa tgacgcataa aaaaagcgcg acatcatgcc gcgcaaagga
    19321 taatcaaata ttaagattta ttttttagaa tgccgaccat ataccgccca ggtaatcact
    19381 acgcaggcga tatagaaaat caaaaatact ttcattgcgc caactggcga acccgttaat
    19441 gccagcgagc taccgaacgc tttcgggata aagaagccac ctatcgcgcc aatcgcagag
    19501 atgaaaccca acgccgccgc cgtgtcggtt gccgcttcac gcatcgcacg ttcgtcagaa
    19561 cccccttctg ctttcacgcg atccattgtc agtttacgga agatcactga aatcatctgg
    19621 aaagtggaac cactacccag cccagctgtc aggaacagcg ccaggaagac cgcgaagaac
    19681 gccatgaagc ttccgccctg cccgtcagta ggtaaggtca ggaacagcag gccgctgaaa
    19741 atcgccatca gaataaagtt caccagcgtg acacgagttc cgcccagacg gtcagataat
    19801 gcaccacctg cagaacgcgc cagcgcacca ataaacggcc cgaagaaagc gtattgcaga
    19861 atctgaacat ccgggaactg cgtttttgac agcatcgcaa agcccgcgga gaagccgata
    19921 aaggagccga aggttgccag atacagcagg ctcataatcc acagatgacc ccgtttgagt
    19981 accggcaact gctccttgat ggaggctttc gaggtagcaa gatcgttcat gccaaaccac
    20041 gctgcaatgg tgaagatggc aaggaacggc acccatatcc aggacgcatt cgccagatac
    20101 agctcagtcc catccggctg tttgacgccc tggctaccaa atactgcgaa aatcgacagt
    20161 gataccacca gcggagcaac caactgcatg acgctgacgc ccatgtttcc cagaccacca
    20221 ttcagaccca gcgcgccacc ctgcttctgt ttcggaaaga agaagctgat gtttgccata
    20281 ctggatgcga agttcgcgcc agcaaagccg catagcagag agatgatgat gaagacgcta
    20341 taaggcgtgg aggtatcctg cacggcaaaa cccagccaga cgcaaggaat aatcagaata
    20401 ccggtgctga acgccgtcca gcgacgacca ccgaagatag gaaccataaa ggagtatgga
    20461 acacgtaata acgcgccaga aaccgaaggc agcgcagtca acataaatag ctgatcggtc
    20521 gtaaaattaa aaccgacttt cggtaagttc accgcaacag cgctgaacaa catccatacg
    20581 caaaacgcca gcagcagaca gggaacggaa atccacaggt tacggctggc aatacgttga
    20641 ccgcgttgtt gccagaacgc aggatcttcc ggtcgccaat ctgtaatgac agctccagta
    20701 gccctttcgg gggcggatga gtgactcata gacacctctg atactcgttt cgatgtctgc
    20761 caccttagtg tctgtagcta aaggcaattt gatgtaaatc aaacgataag ataactttat
    20821 ctatcattga tatttatcat tacccatagt gagtacagtg atttcataaa aattatgaga
    20881 tttttcacgg tgctgtaaaa tccctaccct taccgatgta aagcgactaa ccacacggca
    20941 aataaggagt aactctttcc gggtatgggt atacttcagc caatagccga gaatactgcc
    21001 attccagaat gcatcgtcac attcattaag gttattgctc atttaaagcc tgaaggaaga
    21061 ggtttacatg cttaaacgtt gtctctctcc gctcaccctg gttaatcagg ttgcgcttat
    21121 tgtgttgctt tctactgcta ttggactggc agggatggcg gtttctggct ggctggtgca
    21181 aggcgttcag ggcagcgccc atgcgatcaa caaagcggga tcgctgcgca tgcaaagtta
    21241 ccgtctgttg gcggcagtgc cattaagcga gaaagacaag cccttaatta aagagatgga
    21301 acaaacggca tttagcgccg agttgactcg agcagcagaa cgagacggac aactggcgca
    21361 attacagggt ttacaagatt actggcgtaa tgaactgatc cctgcgctga tgcgtgcaca
    21421 aaaccgagaa acggtgtcag cggatgtcag ccagtttgtt gccgggcttg atcagctggt
    21481 atctggtttt gaccgcacca cggaaatgcg catcgagaca gtggtactgg tccatcgggt
    21541 aatggcggta tttatggcac ttttactggt gttcactatt atctggttgc gggcgcgact
    21601 gctacaaccg tggcggcaac tgctggcaat ggcgagtgcc gtcagccatc gcgattttac
    21661 ccaacgcgca aacatcagcg ggcgcaacga aatggcgatg cttggaactg cgttgaacaa
    21721 tatgtctgca gaactggccg aaagttatgc cgtacttgag cagcgggttc aggagaaaac
    21781 cgccgggctg gagcataaaa atcagatcct ctcattttta tggcaggcta accgccgttt
    21841 gcattcccgc gccccgctgt gtgaacgcct gtcaccggta ctcaacggct tacagaattt
    21901 aaccctgcta cgtgatatcg aactgcgggt ttatgatact gatgatgaag agaatcatca
    21961 ggagtttacc tgccagccag atatgacttg tgatgataaa ggctgccagc tctgcccgcg
    22021 cggcgtatta cccgttggcg atcgcggcac aaccctgaag tggcggctgg ctgactctca
    22081 tacgcagtac ggtattttgc tggcgaccct gccgcagggg cgtcatctta gccatgatca
    22141 acaacaactg gtggataccc tggttgaaca actcaccgcc acgctggcgt tggatcgcca
    22201 tcaggaacgt cagcaacagt tgatcgtgat ggaagagcgt gccaccattg cgcgcgaact
    22261 gcatgattct attgcccaat ctctctcttg tatgaagatg caggtgagtt gtttacagat
    22321 gcagggcgat gcgctgccag aaagcagccg cgaactgtta agtcagatcc gtaacgaact
    22381 gaatgcatcc tgggcgcagt tgcgtgaatt gctcaccaca ttccgcttgc agctcaccga
    22441 gcctggatta cgtccggcgc tggaggcgag ttgcgaagag tacagcgcca aatttggctt
    22501 cccggtgaag ctggattatc aattgccgcc tcgtctggtg ccttcgcatc aggcaatcca
    22561 cttgttgcaa attgcccgtg aggcattaag taacgccctc aaacattcgc aagcgagtga
    22621 ggtcgtggtg acggtggcgc aaaacgataa tcaggtcaaa ctgaccgtac aggataacgg
    22681 ctgcggcgtg cctgaaaatg ccatccgcag caatcactac ggcatgataa taatgcgcga
    22741 ccgtgcgcaa agtttacgag gcgattgccg cgtccgccgt cgtgaatcag gtggcaccga
    22801 agtggtggtc acctttattc ccgaaaaaac tttcacagac gtccaaggag atacccatga
    22861 gtaatcagga accggctact atcctgctga ttgacgatca cccgatgctg cgaactggcg
    22921 taaaacagct tatcagtatg gcaccagata tcaccgtggt tggcgaagcg agtaatggcg
    22981 aacagggtat tgaactggcg gagtctcttg atcccgatct gatcctgtta gatctcaata
    23041 tgcccggcat gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc ctctcagggc
    23101 gcattgtggt attcagcgtc tctaaccatg aagaagatgt ggtcaccgca ctgaaacgcg
    23161 gcgcggatgg ctatctgtta aaagatatgg aaccggaaga tctgctgaaa gcattgcatc
    23221 aggcagctgc tggcgaaatg gtattaagcg aagcattaac gcctgttctg gccgccagct
    23281 tgcgtgctaa ccgtgccact actgagcgcg atgttaacca gttaacccca cgcgagcgcg
    23341 atattctcaa gctgattgcc cagggtttgc cgaacaagat gattgcccgc cgcctggaca
    23401 tcaccgaaag cacagtaaaa gtgcacgtca agcacatgct gaagaaaatg aagctcaagt
    23461 ctcgcgtgga agcagcggta tgggtgcatc aggagcgcat tttctgatta cggttcccag
    23521 cgcagttcgt cgtttgacaa tgcatcgaac ggttcgacaa gcgttagcgt gatctcattg
    23581 gaggagacac gctgcccctg gttatcttcc accaccacag acaatcgcca gtgattgctc
    23641 gccctttccc cgttctgcca gtcaggcatg atcagcgtcc agccctccgc gctgttggct
    23701 tgtgcgcccg gcgtcaaact taatatctgc gtatcgccct gccaaatcag ttgccgaata
    23761 ccgtaacgac tgcggatttg taatttcagc ggcactgttt cgccaggttt tagatcccac
    23821 ggcggtgtcg ccagaaacac cgttaacgtt tttcgctgtc ggtactcaag agtcggtaga
    23881 ttatttcgct gcggattgtc atagcgacta ccacgtaacg actgactttc ggcaacctca
    23941 cccgcagaaa gttgtttttt gagcggtaca ccaaagcggt aattaagatt cagcccgagg
    24001 ttattttgat tctcgccact ttcaccctgt ttatgctggg ccgtcacagt gactaatggc
    24061 acaggggtgt aatttaatcc cagactcaac gcgacgggat tgtgataacc cgtaccagag
    24121 ttaaacaaat caacacgatc accaaaatac tgttctacgc tgacactggt attgaggtgt
    24181 tgatagaacg gcatgcgcat ccgggctgtc aggtcatacc cgcgcgccat ccgttgttcc
    24241 tgcgtggctg tctgttcatg ccatgcagca aacggctgat aaaagtttgc cgatagtcgc
    24301 aaatattcgc cccacgcttc ggcaccaaag cccgctcgct gaagactttc gtccagcaag
    24361 ttgtcataaa aagtgttata acccaccagc cagttgccgc gcgcccagcg ttgcccaacg
    24421 cccacattgc tcaccaaccc atcatcctgc tgagtaagac caagctggct ccaggtgaga
    24481 taacgatcat tatcttgtaa cggcacaaac cagcttccac gactgccggt gaaatgtcct
    24541 tcgttatcca ctttgacgtc aacactggca tttccccacg gtgatagcca ggactctacg
    24601 tgctgattaa cctgttgact aagcgcgtcg cggacttttc ccaatgcgaa agcttttgcc
    24661 tgctcgccag tatccagccc gttatcattc atactggttt cgccgaaatc tttcacaatt
    24721 tcagcaaagt gtttttcccc atcatgattt tcgggagcca tgcctaaatc cggcagacca
    24781 tcattgttat tatcaaaggg atttgccgct ttttgctcga aggtagattg tgcgttagct
    24841 gtaccgcctg ctacaagcaa gagtaaataa aacgggataa tgcgaggaac gaaacggctc
    24901 aaaacgtcgg taacttccgg taataatgaa aaaccatagc cttaaagaat aacgattact
    24961 ggcaactttt gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg tgaccgattg
    25021 gtcagacgct gcagaagcag taagatagaa gatgagtgtc taattccctg aatacaggag
    25081 taaataatgc aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag cgaatccttg
    25141 tttaacagct tgcggctggc cattgcgtta cgagagcagg agagcaatct ggatctgcgt
    25201 ctgttcctca tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc aggggaaggc
    25261 tacaacattc agcaaatgct ggagatcctt accgctcaga atgtaccggt gaaattgtgc
    25321 aaaacctgta ccgacgggcg cgggattagt acacttcctc tgattgatgg ggtggaaatc
    25381 ggtactcttg tggaactggc gcaatggacg ctgtcagccg ataaagtgct cacattttaa
    25441 tgtttgaggg ggcgtctgac gccccactat gcgggaagtt tactcaggcg aatcccggac
    25501 gtaacacacc atccggaaaa ttctccgcca gcagtttttt taccgatacc agcaaatcat
    25561 tcagcccatc gtcctgcatt cccagtttga taagctcctg ttccagtgaa aacaggtatt
    25621 gtgcgttggt tcccagcgga ccgctcgccg ccgcaatcaa cggcgcgatg acctgagcgc
    25681 gagtatcaga ttcatattct ggatgtcgcg gatccataat aaacacaatg gcgtttactg
    25741 tgcgtccatc atcaagatca agctgacacc aggttggcag ataacagccg gtaatcatct
    25801 ctcgcttcca caacaaggtt agttcctgct ccagcgtctc ttctggcagt cgataggcga
    25861 cgcctgtggt gcgtccgccc tctttcagtg caagcatccg tcccggctgg tgcgcagtcc
    25921 cgcgcccggc ggtcaggcgc aggcaaaatg cgcgatgcca tccaaccagt gtaccggtgc
    25981 acgattcggt aaactccagt gccggattcc acatcaacga tccatagccg aagatccaca
    26041 ccggttcctc atcaggtcga caagccagcg tcgccgccag cgaagccgcc cgttgttctg
    26101 ctgaccataa gagtgattct tcaatggcac caaatgccgt tttacaatcg gcattcatca
    26161 agaaatcacg cgttatcacc ttacacctcc gccactgcat acttccctgc gacaactttt
    26221 tggtgtcttc cggacatttt tactgctcat tttgaaacca ctaacgggac aatatgcaat
    26281 ccacggacca cttgcaaggc aacaccgcac gaatagcttt aactaagcta ccggttttac
    26341 gattttttat gccatttatc atcatcccct ttggcatatt catgcttcac agcagcccag
    26401 gccactttat gcgcggtttc ttcgcgactg gcgtcatcac gccgatcgtc tttatcttta
    26461 tattgatccc atgcgctgtt gaacgcttct ttatagatat cctgggcatg agacggtaga
    26521 acgtgcttta cgctttccgg cagatcgctt ttcgttttat acggcatcgt tgactccttt
    26581 tacaaagttg aacattaagt gtggtcaaca atatctactc ttgccaaaac ataagaattt
    26641 tcatgtcaga ctcttcttaa gaaacgctaa aacaaacatt gtctaaacgt aatacttatt
    26701 tttatgcccc aatgagtaaa aaatcgacag cggggtaaaa attcgtaaaa tgttaccgat
    26761 atccagacac tttctgttat tttccctacc tattttttac attttcgata aattcatctg
    26821 caaagggata accataatgt caaatgctca agaggcggta aaaacccgcc acaaggagac
    26881 ttcgcttatt ttcccggttc tggcgctggt agtgctgttc ctgtggggaa gtagccagac
    26941 actaccagtg gtcattgcca tcaatcttct tgcgcttatt ggtattttaa gtagcgcctt
    27001 tagtgttgtc cgtcatgcgg acgtattagc ccatcgcctg ggagaacctt acggttcgct
    27061 tattcttagc ctttcagtgg ttattcttga agtcagtttg atttcagctt taatggcaac
    27121 cggcgacgcc gcgccaacgc taatgcgtga tacgctctat tcaatcatta tgattgttac
    27181 cggtgggctg gttggctttt cattattgtt gggcggtcgt aagtttgcca cccaatatat
    27241 gaatctgttt ggtatcaagc agtatttaat tgcgctgttc cccctggcga taatcgtact
    27301 ggtatttcca atggctctgc ctgcggcgaa tttttcaacc ggtcaggcgt tactggtagc
    27361 attaatttct gcggcaatgt atggcgtatt tttgctgatc cagaccaaaa cgcatcaaag
    27421 tttgtttgtc tacgagcacg aagatgacag tgatgacgat gacccgcatc acggtaaacc
    27481 gtctgcccat agcagcgtgt ggcatgctat ctggttgatt atccatctga ttgccgttat
    27541 tgcagtcacc aaaatgaatg ccagcccgct ggagacattg ctcgacagca tgaatgcccc
    27601 tgtcgccttt actggcttcc tggtggctct gttgattctg tcgccggaag gtttaggtgc
    27661 gttaaaagca gtgttgaaca accaggtcca gcgcgcgatg aatctgttct ttggttcagt
    27721 gttagcaacc atttcgctta ccgtacctgt cgtcacgcta attgccttta tgacgggtaa
    27781 cgaattacag tttgcacttg gtgcgccaga aatggtggtg atggtggcct ctttagtgct
    27841 gtgccatatc tccttctcca ccggacgtac taacgtgctc aatggcgcag cgcatctggc
    27901 actgtttgcc gcctatttga tgacgatatt tgcctgacag ttatatctcc tccgatgacg
    27961 gaaatcgggg gagataagat aaattcctgc ttattgtatt tcttcatact acctggacag
    28021 ctcgttaatc agaattgcga gcctgtacca caacctgcac gaaaccactg caacagctgt
    28081 tgaaaagccg gaatgtactc tttattgcaa acatttcagg gatgaagtga agatgaattg
    28141 agtcgtagta tccacaccaa gccggaatta tccggtgagg cgcaatgttg cgggggcttt
    28201 atccctggtg gcattggttg ctggaaagag aaaacccccg cacgttgaga ggtttaacct
    28261 gacaacaaaa cgggggctaa tcttgaaacc agacacttca agaatagccg caaaatgttg
    28321 tcattacaac aaggcggcta tatgacgctc gcgcagtttg ccatgacttt ctggcacgac
    28381 ctggcagcac cgatcctggc ggggattatt accgcagcga ttgtcagctg gtggcgtaac
    28441 cggaagtaag gtgaaaacgg gcgtcaggct gccgtgatgg caatatgcgc ccgaaaccgg
    28501 ccgcaggggt aacagcctgc ggccattttt gtctttatct cttccacaag taaatattgg
    28561 caacccgagc tggaagcatc atccagagta ctcatgaaac gactgtaaca gctgttgaaa
    28621 aaccggaatg cactcttcat tgcaaacatt tcagggatga agtgaagatg aattgagtcg
    28681 tagtatccac accaagccgg aattatccgg tgaggcgcaa tgttgcgggg gctttatccc
    28741 tggtggcatt ggttgctgga aagagaaaac ccccgcacgt tgagaggtaa aacctgacaa
    28801 cagaacgggg gctaatcttg aaccatcaac aattcaagaa tagccgcaaa atgttgtcat
    28861 tacaacaagg cggctatatg acgctcgcga agtttgccat gattttctgg cacgacctgg
    28921 cagcaccgat cctggcgggg attattaccg cagcgattgt cggctggtgg cgtaaccgga
    28981 agtaaggtga aaacgggcgt caggctgccg tgatggcaat atgcgcccga aaccggccgc
    29041 aggggtaaca gcctgcggcc atttttgtct ttatctcttc cacaagtaaa tattggcaac
    29101 ccgagctgga agcatcatcc agagccctca tgaaacgact gtaacagctg ttgaaaaacc
    29161 ggaatgcact cttcattgca aacatttcag ggatgaagtg aagatgaatt gagacgtagt
    29221 atccacatca agccggcgtt atccggtgag gcgcaatgtt gcgggggctt tatccctggt
    29281 ggcattggtt gctggaaaga gaaaaccccc gcacgttgag aggtttaacc tgacaacaga
    29341 acgggggcta atcttgaacc agaacaattc aagaatagcc gcaaaatgtt gtcattacaa
    29401 caaggcggct atatgacgct cgcgcagttt gccatgattt tctggcacga cctggcagca
    29461 ccgatcctgg cgggaattat taccgcagcg attgtcggct ggtggcgtaa ccggaagtaa
    29521 ggtgaaaacg ggcgtcaggc tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggg
    29581 taacagcctg cggccttttt tgtatctggc acccatgaaa aaagtcttaa cgcagaacgc
    29641 taagacttta tttttcaagc aaaaaagatt acttgctggt atccagttct tcgaaacctt
    29701 tcaccagatc atcaatcgct ttcatctgct tgaggaacgg ttccagttta gccagcggca
    29761 gcgcggatgg accatcacat ttcgcatgtt ccggatccgg atgcgcttca ataaacagcc
    29821 ccgccagacc taccgccata ccggctcgtg ccagctcagc cacctgagca cgacgaccac
    29881 cggaagctgc gccaaacgga tcgcggcatt gcagtgcgtg ggtcacgtcg aaaatcaccg
    29941 gcgagttacc agacactttc ttcataatgc tgaagcccag catatcgaca accaggttgt
    30001 catagccgaa gttagcacca cgatcgcaaa gaatcacttt ttcgttgccg ccttctttga
    30061 atttatcaac gatattaccc atctgtcccg ggctgacaaa ctgtggtttc ttgacgttaa
    30121 ttaccgcacc ggttttcgcc atggcttcaa ccaggtcagt ctggcgagca agaaacgccg
    30181 gcaactgaat cacatccacg acatcagcaa cgggctgtgc ctgacttggt tcgtgaacgt
    30241 cggtgataat tttcacgcca aaagtctgct tcaactcctg gaagattttc atcccttctt
    30301 ccaggcccgg tccacgataa gagtggatgg aggagcggtt ggctttgtca aaagaggctt
    30361 tgaacacgta agggataccc agtttctggg tcacagttac gtagtgctcg caaatgcgca
    30421 tcgccagatc gcgagattcc agcacgttca taccgccaaa cagtacgaac ggcaggtcat
    30481 ttgctacgtt gatgtcgcca atgctaacca ctttttgttt cataggatcg ccttaatctt
    30541 gagtaaaatg tcgattaatt aatgcagcac aatatgttta tgcgcgatgt tatttatttg
    30601 cgcacggatc atttcgctga tcgggtcttc cggacactgt tcaacgaaat aacttaaatc
    30661 gttcaacgca acgtgttcgc aatccagttg cgcataaatc aacccgcgat cgcgaatttc
    30721 atagggatct tcagggttga attgtaataa agcttcgctg gtgcgtaacg ccagctccat
    30781 ctgattttct tccatcaacg aggctttgag tgtatccagc aatttgcgga ttacctcaat
    30841 gttatcagct tcatcaaggt cttcataaaa cagttccgcc gacgggctga tatttccctt
    30901 taaccatacg tccagcatat gttcgcttaa cgattcaccg ttaaaaggat taatcagcca
    30961 aatttcgcca tccggacatt caatgcgcaa tatcagctgc gtagggaaaa tcaccggcag
    31021 cagcggcaaa tcgagacgat tcgcgaccca taataaaacc gcacctaatg ataccgcact
    31081 gccctgtcga ttctttaaca cctggtccag ccataatgca tcggaaagac gataaacgcc
    31141 gcgtgaggct ttaaatcccc agtcgccgta aaacagcgcg atcagttttt ccagctgctc
    31201 ttctaaaggc agaagctggc tgatttcttc cttcgccaga ctaacgagac gctccagttc
    31261 gtcgtaaaca tcttgcgagg gaaaatcgcg gcggattgct tcgcaagcca ggatcatgcc
    31321 ttcgcacaat ggcgctttat taaattcgaa atcagctaac gatctcatga cttaccccag
    31381 taacggtact tttgtggtgg cgagtttaat gatgatgtac agcaccacca acgccagcgg
    31441 gaaggcaata atgcgcgcct gctgactgtg catacgacga taatcgagtg caataaaacc
    31501 caaaacgata taaatgataa ctccaaacag cttttcagtc agccactgtg cctgcccgga
    31561 aaatggcagg atgtgcgctt tagccatcaa cgcaatgccg cttaacaata acaccgtatc
    31621 gacaaccggc ggcacaatgc gcgtccagcg agcaaatgcc tgtggatgct tctgataacg
    31681 tagccagaaa cgtaaggtta atagcccaac agaaagcgcg atactaataa gatgaacact
    31741 aagcagtgta gaaaaacttg tcattgataa tagcggccga gcgttacgcg ctcgttatca
    31801 ccatagtcac ggcaggtttc gacgtcatga taccccgcga ggataaatgc ttgtcgcacc
    31861 gcttcgccct gctgccagcc atgttccaga agcagaaagc cgccggatac cagcgcgtta
    31921 cgcgactgtt cgatgatatg cacgatgtct gccattccac tgtctgccgc aaccagcgca
    31981 gtgagcggct caaagcggac atcgccttgt tgaagatgtg ggtcctgctc gtcaatatac
    32041 ggcggattgc tgacaatcat cgcaaactgc tgcccggcta gcgcgctaaa ccagtcgctt
    32101 tgcagaatgt ggatattttt gatcgccaga tgctgggcat tacgttgtgc cagggagaca
    32161 gcatcaggca tacgatctac agcgataatt tcgcagtccg ggcgctcgct agccagcgca
    32221 agcgcaatcg ccccggtacc cgtcccgaga tcgagaatac ggcaaggttg ttcaggcaac
    32281 cgcgccagtg cctgctccac cagacactcc gtatccgggc gcggaattaa ggtcgctggc
    32341 gaaacaaata acggcaacga ccagaattct cgcaccccgg ttaaatgagc aatgggttca
    32401 ccatcgcgac gacgtgtcag tagcgcatca agttgctgac attgttcgtc agtcagctgc
    32461 gtttcaccaa aggccagaat aaaagtccgc cccctgccag tgacatactc caataagatt
    32521 tcagcatcac gccgcgggct ttcgctcgcc tgaagttggc ttattgcttc acgtaaccag
    32581 tgttgatatt ccattattcc tgctcggaca acgccgccag ttggtcggcc tgatgttcct
    32641 ggataatcgg ttcaatcagc atatccagct taccttccat cacttcatcc aggcggtaga
    32701 gcgtcaggtt gatgcggtga tcggtaacgc gcccctgcgg gaaattgtaa gtacggttac
    32761 ggtcgctgcg atcgccactc cccagcaggt tacgacgggt agacgcttcg gcctgttggc
    32821 gttttgccat ttcagcagcg tggatgcgag caccgagcac tgaaagcgcc ttcgctttgt
    32881 ttttatgttg tgaacgttca tcctgacatt caacaactat cccggtcggc aagtgagtaa
    32941 tacgaattgc cgaatcggtg gtgttaacgt gctgaccacc cgcccctgac gagcggaaag
    33001 tatcaatgcg taaatctgct gggttgatgt ccggcagttc tgcgtcaggc agttctggca
    33061 ttaccgcaac ggtacaagca gaagtatgaa tacgaccctg cgattccgta gcaggaacac
    33121 gctgcacgcg atgaccgcca gattcaaatt tcagacgacc atacacacca tcaccgctaa
    33181 ttttggcgat gatctcttta taaccaccat gttcaccctc gctggcgctc atgatttcta
    33241 cccgccagcg gcgggcttcg gcgtaacggc tgtacatacg gaacagatcg cctgcgaaca
    33301 gcgccgcttc gtcgccgccg gttccggctc ggacttcgag gaaggcgtta cgttcgtcat
    33361 caggatcttt tggcagtaac agaacctgta attgctgttc cagttgctcg cttttttctt
    33421 tagcttcgcg cagttcatcc tgcgccatct cacgcatttc aggatcatcg agcatcatct
    33481 gtgcggtttc gatatcttcc tgaacctgtt gccagtcggt aaaacagcgc gaaacatcac
    33541 ttaactgcgc atattcgcgt gataatgcgc gaaaacgttc ctggtcggcg atagtttgcg
    33601 cgtcacccag caacgcctga acttcttcat ggcgttcatg cagggcttcc agtttggcaa
    33661 cgatagaagg cttcataggc gtaaatgcac cctgtaaaaa aagaaaatga tgtactgcta
    33721 ctccagcccg aggctgtcgc gcagaatatt caggcgttcg ttatccccgt cacgggcggc
    33781 ctgttgaagt gatttcgttg gcgcatggat caagcggtta gtcagtttcc atgccagatc
    33841 ctgcataatg gcttgcgcgt cgccgccctg ctcaagggcc gctaacgctt tggcggttaa
    33901 ctcatcgcga acgtgctctg cctggctgcg atactcgcga atggtttcgc tggcgctttg
    33961 tgctcgcagc cacgccataa attcgctggt ttcctgagcg acaatagttt ccgcctcaac
    34021 cgctgcggct ttacgctgcg ccaggttgtg cgaaatgatg ctttgcagat cgtccacact
    34081 ataaagataa gcattcgcca gtttgccaac ttccggctca acatcgcgcg gaacggcaat
    34141 atccaccaac agcattggtt ggttgcgacg gctttttaat gcgcgctcca ccatgccttt
    34201 cccgataatc ggtaacgggc tggcggtgga actgatgatg atatcggctt cgcgcagacg
    34261 ttcgtcgatc tcactcaggg caatcacttc cgcgccaact tcatctgcca gaatttgggc
    34321 acgttcgcga gtgcggttgg cgataatcat cttctgtact ttatgttcgc gcagatgacg
    34381 cgctaccagc tcgatggttt cgcccgcgcc taccagcaac actgtgaccg tagagagcga
    34441 ttcaaagatc tgccgcgcca gcgtacaagc cgcaaaagcg acagacacag cactggcacc
    34501 gatatctgtt tcagtgcgaa cgcgtttcgc tacagagaaa gatttctgga acatgcgttc
    34561 cagttcgctg gccttcatat gacctttttg cgaatcggca aacgcttttt taacctgacc
    34621 gaggatctgc ggctccccaa gaaccagtga atccaggccg ctggcaacac gcattaaatg
    34681 gctaaccgcg tcgttatcct gatgccagta gaggctttta cgcagatctt cttcattaag
    34741 attgtgataa tcgcaaagcc agcggattaa cgcctcttgc aggttatcct gctcttcaac
    34801 gctaagataa agttccgtgc ggttgcacgt cgacagcacc acgccgccct gcaccatcgg
    34861 ctgcgcaagc aggctgtcaa gcgcctgatc gagcttatcc ggcgaaaacg atacacgttc
    34921 tcgcagcgat acaggtgccg ttttatggtt gataccgagt gctaaaaggg tcatgtctgc
    34981 gggaaataat accaacgttg atagggttag tctgcttgca tcatacagga tgcgtaagat
    35041 caataaaaga gagcgccccc ttttggagta attgccgaaa gccgttagat tctggcaatt
    35101 aagacaactt gaacatagac gatagcggac ggtaacgcta gcattaaggg ttataactgc
    35161 aacgtatctc aaggacttgt catcactatg cccctgcccg attttcgtct tatccgcctg
    35221 ctaccgctgg ctgctcttgt gcttactgcc tgttccgtta ccacgcccaa aggtcctggc
    35281 aaaagcccgg attcgccaca atggcgtcag catcagcaag acgtgcgcaa tcttaatcag
    35341 tatcagactc gcggcgcgtt cgcttatatt tctgaccaac aaaaagtgta cgcccgcttt
    35401 ttctggcagc aaaccggcca ggatcgctac cgtctgctgc tcactaaccc attgggcagc
    35461 acggaactgg agctgaatgc tcaaccgggt aacgtgcagt tagtcgacaa taaaggtcag
    35521 cgttataccg ccgatgacgc cgaagagatg attggcaaat tgaccggaat gccaattccg
    35581 ctcaacagct tgcgccagtg gattttaggt ttaccgggtg atgcaaccga ctacaaactg
    35641 gacgaccagt accgcctgag cgaaattacc tacagccaga atggcaaaaa ctggaaggtt
    35701 gtttatggtg gttatgacac caaaacgcaa cctgcgatgc cagccaatat ggaactcacc
    35761 gacggtggtc aacgcatcaa gttaaaaatg gataactgga tagtgaaata atgcggacac
    35821 agtggccctc tccggcaaaa cttaatctgt ttttatacat taccggtcag cgtgcggatg
    35881 gttaccacac gctgcaaacg ctgtttcagt ttcttgatta cggcgacacc atcagcattg
    35941 agcttcgtga cgatggggat attcgtctgt taacgcccgt tgaaggcgtg gaacatgaag
    36001 ataacctgat cgttcgcgca gcgcggttgt tgatgaaaac tgcggcagac agcgggcgtc
    36061 ttccgacggg aagcggtgcg aatatcagca ttgacaagcg tttgccgatg ggcggcggtc
    36121 tcggcggtgg ttcatccaat gccgcgacgg tcctggtggc attaaatcat ctctggcaat
    36181 gcgggctaag catggatgag ctggcggaaa tggggctgac gctgggcgca gatgttcctg
    36241 tctttgttcg ggggcatgcc gcgtttgccg aaggcgttgg tgaaatacta acgccggtgg
    36301 atccgccaga gaagtggtat ctggtggcgc accctggtgt aagtattccg actccggtga
    36361 tttttaaaga tcctgaactc ccgcgcaata cgccaaaaag gtcaatagaa acgttgctaa
    36421 aatgtgaatt cagcaatgat tgcgaggtta tcgcaagaaa acgttttcgc gaggttgatg
    36481 cggtgctttc ctggctgtta gaatacgccc cgtcgcgcct gactgggaca ggggcctgtg
    36541 tctttgctga atttgataca gagtctgaag cccgccaggt gctagagcaa gccccggaat
    36601 ggctcaatgg ctttgtggcg aaaggcgtta atctttcccc attgcacaga gccatgcttt
    36661 aagccgggca agctgagttt cggtgacaac gtcaccttgt tccagacgtt gcatcgcgct
    36721 ctttaataca ccgcctggaa aggatcatgc ctggcccgca cagttttcgg cagattcttt
    36781 ccaccaatgg acgcatgcct gaggttcttc tcgtgcctga tatgaagctt tttgctggta
    36841 acgccacccc ggaactagca caacgtattg ccaaccgcct gtacacttca ctcggcgacg
    36901 ccgctgtagg tcgctttagc gatggcgaag tcagcgtaca aattaatgaa aatgtacgcg
    36961 gtggtgatat tttcatcatc cagtccactt gtgcccctac taacgacaac ctgatggaat
    37021 tagtcgttat ggttgatgcc ctgcgtcgtg cttccgcagg tcgtatcacc gctgttatcc
    37081 cctactttgg ctatgcgcgc caggaccgtc gcgtccgttc cgctcgtgta ccaatcactg
    37141 cgaaagtggt tgcagacttc ctctccagcg tcggtgttga ccgtgtgctg acagtggatc
    37201 tgcacgctga acagattcag ggtttcttcg acgttccggt tgataacgta tttggtagcc
    37261 cgatcctgct ggaagacatg ctgcagctga atctggataa cccaattgtg gtttctccgg
    37321 acatcggcgg cgttgtgcgt gcccgcgcta tcgctaagct gctgaacgat accgatatgg
    37381 caatcatcga caaacgtcgt ccgcgtgcga acgtttccca ggtgatgcat atcatcggtg
    37441 acgttgcagg tcgtgactgc gtactggtcg atgatatgat cgacactggc ggtacgctgt
    37501 gtaaagctgc tgaagctctg aaagaacgtg gtgctaaacg tgtatttgcg tacgcgactc
    37561 acccgatctt ctctggcaac gcggcgaaca acctgcgtaa ctctgtaatt gatgaagtcg
    37621 ttgtctgcga taccattccg ctgagcgatg aaatcaaatc actgccgaac gtgcgtactc
    37681 tgaccctgtc aggtatgctg gccgaagcga ttcgtcgtat cagcaacgaa gaatcgatct
    37741 ctgccatgtt cgaacactaa tcgaacccgg ctcaaagacc cgctgcggcg ggtttttttg
    37801 tctgtaatat ccatttgtat gacctatgcc tccttcacct gccatttagt tgacagatga
    37861 tgcgctcacg gatgaaacat tattgtgaac aaaatatttt cctcacatgt gatgcctttc
    37921 cgcgctctga tcgacgcttg ctggaaagaa aaatatactg ccgcacggtt tacccgtgac
    37981 ctgattgccg ggataaccgt cgggattatt gctatcccgc tggcgatggc gttggctatt
    38041 ggtagtggtg tggcacccca gtacggttta tataccgcag ctgttgcggg gattgtcatt
    38101 gctctgacgg gtgggtcacg ctttagcgtt tccggtccga ctgcggcatt tgtggtaatt
    38161 ctctatcccg tgtcgcaaca gtttggactg gcaggactgc tggttgcgac cttgctgtcg
    38221 gggatttttt tgattctgat gggtctggca cgctttggtc gcctgattga gtatattccg
    38281 gtttccgtca ccttaggttt cacctcgggt atcgggatca ccatcggtac catgcagatt
    38341 aaagattttc tcggtctgca aatggcccat gtcccggaac attatctaca aaaagtcggc
    38401 gcattattta tggcgctgcc gaccattaat gtgggtgatg ctgccattgg cattgtgacg
    38461 ctaggtattc ttgttttctg gccgcgtctg ggcattcgtt tacccggtca ccttccggcc
    38521 ttgctggctg gttgcgcggt gatggggatt gttaacctgc tcggcggaca tgttgctacc
    38581 atcggttcgc aattccacta cgtcctggcc gatggttctc agggtaacgg tattccgcaa
    38641 ctgctgccgc aactggtgct gccgtgggat ctgcctaatt cagaattcac gctaacctgg
    38701 gattctattc gcacactgct gcctgcggca ttctcaatgg caatgctcgg cgcaatcgaa
    38761 tctctgctct gcgccgtggt gctggatggt atgaccggga cgaaacacaa ggcgaatagc
    38821 gaactggttg gacagggact ggggaatatc atcgctccgt tctttggtgg cattaccgct
    38881 acagctgcca tcgcgcgttc tgccgctaac gtccgtgccg gggcaacttc ccctatctcg
    38941 gcggtgatcc actctattct ggttattctt gccctgctgg tactggcacc gctgctctcc
    39001 tggctgccgc tttccgccat ggctgccctg ctgttgatgg tggcgtggaa catgagtgaa
    39061 gcgcacaaag tggtcgactt gctgcgtcat gcgccgaaag atgacatcat tgtcatgctg
    39121 ctgtgcatgt cgctgaccgt gctgtttgat atggttattg ccatcagcgt ggggatcgtg
    39181 ctggcatcgc tgctgtttat gcgtcgtatc gcacgtatga ctcgcctggc accggtagtc
    39241 gtagatgttc cagacgatgt cctggttctg cgcgttattg gcccgctgtt ttttgctgct
    39301 gctgaaggct tgttcacgga cctggagtca cgtcttgaag gcaaacggat tgtgattctg
    39361 aagtgggatg cggttccggt acttgatgct ggtggtcttg atgcgttcca gcgttttgtg
    39421 aagcgtctgc ccgagggatg tgaactgcgc gtgtgcaacg tggaattcca gccactgcgc
    39481 actatggctc gcgctggcat tcaaccgatc ccgggacgcc tggcgttctt cccgaatcgt
    39541 cgcgcggcga tggcggattt ataagtacga gattgaccag tcagcgcaaa ctgactggtc
    39601 agcaaactgc attatttgtt agctatgacg gcgattatcg ctacggcggc aacgtttgtc
    39661 atagtggcga acccaccagt aacggtcgca cacctgttca tgcccgccaa cacgggcacc
    39721 cgccagccag agaatggcac cgacgaaaat acttagcact gcaccatgtg cgaaatactg
    39781 gggcatatta aactgtggta actggttgag gattgaatac cccacgccga ccaccattac
    39841 caccagaccc aaccccatga gcacgttacc gagtaacgaa gcgtttttgc gtttcatatg
    39901 tcacctccgg aactttctgg gttgtaacag ggaatacccc tcttccttat gtgtaaagta
    39961 tagacaacac cctgtgggtt atgtgcgggc gtgatcacaa ttacaaccct tatttcaaca
    40021 aaactttaca aataaacgcc tgaacacctt gactttctaa tagtccactc atgcaattat
    40081 tacgttatct gcatttgatt cgcagttttt tgtcaagcgg ggccgcaacc agtaaactac
    40141 gcgccagtta tgtacacact caggacaaaa aaacgtgacg attaaattga ttgtcggcct
    40201 ggcgaacccc ggtgctgaat acgccgcaac gcgacataat gctggtgcct ggttcgttga
    40261 cttactggca gagcgtttgc gcgctccgct gcgcgaagag gctaaattct ttggttatac
    40321 ttcgcaagtc actcttggag gcgaagatgt ccgcctgtta gtcccgacta catttatgaa
    40381 tctcagcggc aaagccgttg cggcgatggc cagttttttc cgcattaatc cggacgaaat
    40441 tctggtggcc cacgacgaac tggatctgcc tcctggcgtc gccaaattta aattgggcgg
    40501 tggccatggt ggtcacaatg gactgaaaga catcatcagt aaattgggta ataaccctaa
    40561 ctttcaccgt ttacgcatcg gaatcggtca tccgggcgat aaaaataaag ttgtcggttt
    40621 tgtgttaggc aaaccgcctg ttagtgaaca gaagttaatt gatgaagcca tcgacgaagc
    40681 ggcgcgttgt actgaaatgt ggtttacaga tggcttgacc aaagcaacga accgattgca
    40741 cgcctttaaa gcgcaataag tcgttgtctg cggcattttt gccgagtgcc gtgtataata
    40801 ggcaaagtta tttccatttc tgcaatctgt tagcaataac aggttgatta ttaagatatt
    40861 aaggtgattt aaatcatggg attcaaatgc ggtatcgtcg gtttgcccaa cgtcgggaaa
    40921 tctaccctgt tcaacgcgct gaccaaagcc ggtattgaag cggccaactt tccattctgc
    40981 accattgagc cgaacacagg cgtcgtacca atgcctgacc ctcgcctgga tcaactggct
    41041 gaaatcgtaa aaccgcaacg tacgcttccc acgaccatgg aatttgtcga tatcgccggt
    41101 ctggtaaaag gcgcatcgaa aggcgaaggt ctgggtaacc agttcctgac caacatccgt
    41161 gaaaccgaag cgatcggtca cgttgttcgc tgctttgaaa atgacaacat cattcacgtt
    41221 tccggcaaag ttaacccggc tgacgatatt gaagttatca acaccgaact ggcgctggca
    41281 gacctcgaca cctgcgaacg tgcgattcat cgcgtacaga agaaagccaa aggtggcgat
    41341 aaagacgcga aagctgagct ggcggtcctg gaaaaatgcc tgccccagtt ggaaaatgca
    41401 ggtatgctgc gcgcgctgga tttaagcgct gaagagaaag cggctattcg ttacctgagc
    41461 ttcctgacgc tgaaaccaac aatgtacatc gccaacgtca acgaagacgg ttttgaaaac
    41521 aacccatatc ttgaccaggt gcgtgaaatc gcggcgaaag aaggttctgt tgtggttccg
    41581 gtttgtgctg ctgttgaagc agacattgcc gaactggacg acgaagaacg tgacgagttt
    41641 atgcaggagc ttgggctgga agagccgggc ctgaaccgtg tgatccgtgc cggttataag
    41701 ctgctgaacc tgcaaactta cttcaccgct ggggtgaaag aagtgcgtgc atggaccatt
    41761 ccggttggag caaccgcgcc gcaggcagcg ggcaaaatcc atactgattt tgaaaaaggc
    41821 tttatccgtg cacaaaccat ctcgtttgaa gatttcatca cttacaaagg tgaacaaggc
    41881 gcgaaagaag caggcaaaat gcgtgcagaa ggtaaagatt acatcgttaa agatggcgat
    41941 gtgatgaact tccttttcaa cgtctaatta atttgttgtc tcatgaggtt tcgctaaatc
    42001 tcatgaagag cgcaaaagta tataaaatcc acgcaattgc gtggattttt catttcaccg
    42061 agtcctaatc attcttgtaa catcctacac ttttgtgctt attgcctata gtgaaccacg
    42121 agaataatta aagacataag tgttcagatg acggatatct cattagcagt gatataacgt
    42181 aagtttttgt atcactacac atcagccacc ttcacagctg atcaaaattg atcacgctcc
    42241 aatatgctgg caagatgaaa atgataacca gtagcacaat aacaacgata ggccagaaaa
    42301 cccattgacc aaaaatgaat atcgatgttt tttgcttttt cttattagta atgcaaaaat
    42361 ggcgcatatt gtttttgtta tgcagataca tcaagcgaaa ttcccataac taattaggat
    42421 tattacaaaa aatc
//
LOCUS       DAZFRN010000022        42239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFRN010000022 DAZFRN010000000
VERSION     DAZFRN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.022"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            119..1339
                     /locus_tag="ACHKUB_001178"
     CDS             119..1339
                     /locus_tag="ACHKUB_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6654220.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQFQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1312..1941
                     /locus_tag="ACHKUB_001179"
     CDS             1312..1941
                     /locus_tag="ACHKUB_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO6654221.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1942..3102)
                     /gene="ybdL"
                     /locus_tag="ACHKUB_001180"
     CDS             complement(1942..3102)
                     /gene="ybdL"
                     /locus_tag="ACHKUB_001180"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO6654222.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3211..4299
                     /gene="hcxA"
                     /locus_tag="ACHKUB_001181"
     CDS             3211..4299
                     /gene="hcxA"
                     /locus_tag="ACHKUB_001181"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO6654223.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4309..4506)
                     /locus_tag="ACHKUB_001182"
     CDS             complement(4309..4506)
                     /locus_tag="ACHKUB_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6654224.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4688..6793)
                     /gene="cstA"
                     /locus_tag="ACHKUB_001183"
     CDS             complement(4688..6793)
                     /gene="cstA"
                     /locus_tag="ACHKUB_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO6654225.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSLG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6974..7387)
                     /gene="entH"
                     /locus_tag="ACHKUB_001184"
     CDS             complement(6974..7387)
                     /gene="entH"
                     /locus_tag="ACHKUB_001184"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO6654226.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7390..8136)
                     /gene="entA"
                     /locus_tag="ACHKUB_001185"
     CDS             complement(7390..8136)
                     /gene="entA"
                     /locus_tag="ACHKUB_001185"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO6654227.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8136..8993)
                     /gene="entB"
                     /locus_tag="ACHKUB_001186"
     CDS             complement(8136..8993)
                     /gene="entB"
                     /locus_tag="ACHKUB_001186"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO6654228.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9007..10617)
                     /gene="entE"
                     /locus_tag="ACHKUB_001187"
     CDS             complement(9007..10617)
                     /gene="entE"
                     /locus_tag="ACHKUB_001187"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO6654229.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALIEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10627..11802)
                     /gene="entC"
                     /locus_tag="ACHKUB_001188"
     CDS             complement(10627..11802)
                     /gene="entC"
                     /locus_tag="ACHKUB_001188"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO6654230.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11991..12947
                     /gene="fepB"
                     /locus_tag="ACHKUB_001189"
     CDS             11991..12947
                     /gene="fepB"
                     /locus_tag="ACHKUB_001189"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6654231.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12951..14201)
                     /gene="entS"
                     /locus_tag="ACHKUB_001190"
     CDS             complement(12951..14201)
                     /gene="entS"
                     /locus_tag="ACHKUB_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO6654232.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14312..15316
                     /gene="fepD"
                     /locus_tag="ACHKUB_001191"
     CDS             14312..15316
                     /gene="fepD"
                     /locus_tag="ACHKUB_001191"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO6654233.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15313..16305
                     /gene="fepG"
                     /locus_tag="ACHKUB_001192"
     CDS             15313..16305
                     /gene="fepG"
                     /locus_tag="ACHKUB_001192"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO6654234.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16302..17117
                     /gene="fepC"
                     /locus_tag="ACHKUB_001193"
     CDS             16302..17117
                     /gene="fepC"
                     /locus_tag="ACHKUB_001193"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6654235.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17114..18247)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKUB_001194"
     CDS             complement(17114..18247)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKUB_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO6654236.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18463..22344)
                     /gene="entF"
                     /locus_tag="ACHKUB_001195"
     CDS             complement(18463..22344)
                     /gene="entF"
                     /locus_tag="ACHKUB_001195"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO6654237.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSEKIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLVQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22341..22559)
                     /gene="ybdZ"
                     /locus_tag="ACHKUB_001196"
     CDS             complement(22341..22559)
                     /gene="ybdZ"
                     /locus_tag="ACHKUB_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO6654238.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22562..23764)
                     /gene="fes"
                     /locus_tag="ACHKUB_001197"
     CDS             complement(22562..23764)
                     /gene="fes"
                     /locus_tag="ACHKUB_001197"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO6654239.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24007..26247
                     /gene="fepA"
                     /locus_tag="ACHKUB_001198"
     CDS             24007..26247
                     /gene="fepA"
                     /locus_tag="ACHKUB_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO6654240.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNHWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26422..27042
                     /gene="entD"
                     /locus_tag="ACHKUB_001199"
     CDS             26422..27042
                     /gene="entD"
                     /locus_tag="ACHKUB_001199"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO6654241.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27164..27316)
                     /gene="hokE"
                     /locus_tag="ACHKUB_001200"
     CDS             complement(27164..27316)
                     /gene="hokE"
                     /locus_tag="ACHKUB_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6654242.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27667..27819)
                     /gene="hokE"
                     /locus_tag="ACHKUB_001201"
     CDS             complement(27667..27819)
                     /gene="hokE"
                     /locus_tag="ACHKUB_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6654243.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            27788..28057
                     /locus_tag="ACHKUB_001202"
     CDS             27788..28057
                     /locus_tag="ACHKUB_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654244.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            28261..29379
                     /locus_tag="ACHKUB_001203"
     CDS             28261..29379
                     /locus_tag="ACHKUB_001203"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO6654245.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCENERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29445..29693
                     /locus_tag="ACHKUB_001204"
     CDS             29445..29693
                     /locus_tag="ACHKUB_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO6654246.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29758..30126
                     /locus_tag="ACHKUB_001205"
     CDS             29758..30126
                     /locus_tag="ACHKUB_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6654247.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30220..30873
                     /gene="nfsB"
                     /locus_tag="ACHKUB_001206"
     CDS             30220..30873
                     /gene="nfsB"
                     /locus_tag="ACHKUB_001206"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO6654248.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            30981..32228
                     /gene="ybdG"
                     /locus_tag="ACHKUB_001207"
     CDS             30981..32228
                     /gene="ybdG"
                     /locus_tag="ACHKUB_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO6654249.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32296..33672)
                     /gene="pheP"
                     /locus_tag="ACHKUB_001208"
     CDS             complement(32296..33672)
                     /gene="pheP"
                     /locus_tag="ACHKUB_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO6654250.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33774..36917)
                     /gene="cusA"
                     /locus_tag="ACHKUB_001209"
     CDS             complement(33774..36917)
                     /gene="cusA"
                     /locus_tag="ACHKUB_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO6654251.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(36929..38152)
                     /gene="cusB"
                     /locus_tag="ACHKUB_001210"
     CDS             complement(36929..38152)
                     /gene="cusB"
                     /locus_tag="ACHKUB_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO6654252.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQTEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(38168..38500)
                     /gene="cusF"
                     /locus_tag="ACHKUB_001211"
     CDS             complement(38168..38500)
                     /gene="cusF"
                     /locus_tag="ACHKUB_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO6654253.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38524..39906)
                     /gene="cusC"
                     /locus_tag="ACHKUB_001212"
     CDS             complement(38524..39906)
                     /gene="cusC"
                     /locus_tag="ACHKUB_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO6654254.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40063..40746
                     /gene="cusR"
                     /locus_tag="ACHKUB_001213"
     CDS             40063..40746
                     /gene="cusR"
                     /locus_tag="ACHKUB_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO6654255.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40736..42184
                     /gene="cusS"
                     /locus_tag="ACHKUB_001214"
     CDS             40736..42184
                     /gene="cusS"
                     /locus_tag="ACHKUB_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO6654256.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10078 a        11715 c        10809 g         9637 t
ORIGIN      
        1 ttcatctatt tattcacagc gcgaatgaaa aataaaacac tctgctttgc agtgtttact
       61 ttataattaa cagacttatc tcactgatca ccctgtaacg ttcagagagc gtcttcagat
      121 gtctatttat aaaattcctc ttccgctcaa tattctggaa gctgcacgag agcgtattac
      181 ctggacactc aacaccctac cccgcgtttg tgtctcattt tctggcggca aagactctgg
      241 tttaatgctg catctgacag ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt
      301 gtttatcgac tgggaggcgc aattctcttg caccattaac tatgttcagt ccctgcgcga
      361 gttgtacacc gatgtcatcg aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa
      421 ttccctttca caattccaac ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg
      481 tcagcccccg caagatgcga taaccgatcc tgactttttc tgcttttacc agcctggcat
      541 gaccttcgaa caatttgtac gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc
      601 gatgatgatc ggcatccgtg cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt
      661 aaataaacaa cgttttgccg acgataaacc atggactacg gccgcaccag gcggtcatag
      721 ctggtacatt taccccattt acgactggaa agtggctgat atctggacct ggtatgcaaa
      781 tcatcaaagt ctctgcaacc cactgtataa cctaatgtat caggcaggcg ttcctctgcg
      841 tcatatgcga atttgcgaac cttttggccc ggagcaacga caaggattat ggctctatca
      901 cgttatcgaa ccggatcgct gggcagctat gtgcgcacga gtcagcgggg taaaaagtgg
      961 cggcatttac gccggacatg acaatcattt ctatgggcat cggaaaatcc tcaagccaga
     1021 acatttagac tggcaagaat atgcattatt gctgctcaat agcatgccgg aaaaaacagc
     1081 tgagcattac cgcaataaaa tcgccattta tttgcactgg tatcagaaaa aaggcatcga
     1141 ggttccacaa acccagcaag gggacattgg cgcgaaagat atcccctcct ggcggcggat
     1201 atgcaaagtt ttactcaata acgattactg gtgtcgagca ttatcattta gtcctacgaa
     1261 agcgaagaac tatcagcgtt ataacgaacg gataaaagga aaacgtcagg aatgggggat
     1321 actatgcaac aacgattaac gcaggattta acgcaatttc tcgccagctt gccggaagat
     1381 gatcgcatca aagcgattaa tgaaatccgc atggcgatcc atcaggttag cccctttcgt
     1441 gaagagccag tggattgcgt tctgtgggtt aaaaacagcc aacttatgcc taatgattac
     1501 aacccaaaca acgtggcacc acccgaaaaa aaactgctga aaaaatccat tgaaatagat
     1561 ggttttaccc aacccattgt ggtcacgcat acggataaaa atgccctgga aatcgtggat
     1621 ggttttcacc gccatgaaat tggtaaaggc tcatcatcat taaaattacg cttaaaaggt
     1681 tatttgccgg tgacctgtct ggaaggtacg cgtaatcaac gcatcgccgc gactattcgt
     1741 cataatcgcg ctcgtggacg ccatcaaatc accgccatgt cggaaatcgt ccgtgaactt
     1801 agccagttgg gatgggacga taataaaatt ggcaaagagc tgggaatgga cagcgacgaa
     1861 gtgctgcgcc tgaagcaaat taacggcctg caagagttgt ttgccgaccg tcaatattcc
     1921 cgcgcctgga cggttaaata gctaaagctg gcgcaggcgt tcagctgctg ccagcaacgt
     1981 cgattccttc ttggcaaaac agagacgaat cagtttatgt gggaagggat cggcgcaaaa
     2041 caccgacagc ggaatcgccg ctacgccgtg ctcctgcgtc agccactggc aaaactcaac
     2101 atcatccagg gtagaaaccg cgctgtaatc caccagcaaa aagtatgtac cttcacacgg
     2161 taaaatctcc agccggcttt catttaaagc attcaccaga atatcgcgct tctggcgata
     2221 aaagtccggt aacgcaagat aatgctcagg ttctgcacgt agcatatcag caagcgccag
     2281 ctgtgccggg gtattcaccg aaaaggtcag atactgatgt accttgcgaa tttcggcgct
     2341 gatgggcgct ggcgcaacac aataacccac tttccagccg gtcatatgat aggtcttgcc
     2401 aaatgaagaa accgccactg cccgctcacg cagctgcgga tgcgccagca cactggcatg
     2461 gccctgttgt gaaaagttga tgtgctcgta gacttcatcg ctaatgacaa aaatctcgtg
     2521 cccggcgatc gcctgccaca aagcggcgaa atcagcctgc tgccagatag ttgcactggg
     2581 gttatgcgga gtgttgagga tcaccagtct ggtgcgctcg cttaataatg cggcaaattc
     2641 ctgccagtca acgcgaaaat gcggtggttg cagtgccata cgcttcacta ttcccccaga
     2701 aagcgcgatg gcgggggagt aactgtcata gctgggatca aaacaaatca cttcatcgcc
     2761 attgcgcacc agtgcggtaa tcgccgcgta taacgcttcc gtcgcccctg ccgttacggt
     2821 gatatcgcta tcggcatctg gttgatagcc atacaaacgt tccgttttct gagcaatcgc
     2881 ctcgcgcaag gcctgcacgc cggtcatggg cgcgtattgg tttgccccct gggcaacgtg
     2941 gtacgccagc cgctcctgta aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag
     3001 gttaatcgcc tggtgttgct gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag
     3061 ttgtggaagt ttgctttgtg gaatcagagg gttatttgtc atttttattg tacctgacga
     3121 agttgcgtgt tgaaggcact ataacacgat gctagtattt ggcaatcaag acgtttagat
     3181 gtctaaataa aacaataagg acaacacaac atgcctcaca atcctatccg cgtggtcgtc
     3241 ggcccggcta actacttttc acatccagga agtttcaatc acctgaacga ttttttcact
     3301 gatgaacaac tttctcgcgc ggtgtggatc tacggcaaac gcgccattgc tgcggcgcaa
     3361 accaaacttc cgccagcgtt tggacttcca ggggcaaagc atattttgtt tcgtggtcat
     3421 tgcagcgaaa gcgatgtaca gcaactggcg gctgagtccg gtgacgatcg cagcgtggtg
     3481 attggcgtcg gcggcggcgc actgctcgac accgcgaaag ccctcgcccg ccgtctcggt
     3541 ctgccatttg ttgccgctcc gacgatcgcc gccacctgcg cagcctggac accgctctcc
     3601 gtctggtata acgatgccgg acaggcgctg cattatgaga ttttcgacga cgccaatttt
     3661 atggtgctgg tggaaccgga gattatcctc aatgcaccgc aacaatatct gctggcgggg
     3721 atcggtgaca cgctggcgaa atggtatgaa gcggtggtgc tggctccgca accagaaacg
     3781 ttgccgctaa ccgtgcggct ggggatcaat aatgcgcagg ccattcgcga cgtcttgtta
     3841 aacagtagcg aacaggcact tgccgatcag caaaatcagc agttaacgca atcattttgc
     3901 gatgtggtgg atgcgattat cgccgggggt ggaatggtcg gtggtctggg cgatcgtttt
     3961 acgcgtgtgg ctgcggccca tgccgtgcat aacggtctga ctgtcctgcc gcaaaccgag
     4021 aaatttctcc acggcaccaa agtcgcttac ggaattctgg tgcaaagcgc cttgctgggt
     4081 caggatgatg tgctggcgca attaaccggg gcgtatcagc gttttcatct gccgactacg
     4141 ctggcggagc tggaagtgga tatcaataat caggcggaga tcgacaaagt gattgcccac
     4201 accttgcgcc cggtggagtc cattcattac ctgccagtca cgctgacacc agatacgttg
     4261 cgtgcagcgt tcgaaaaagt ggaatcgttt aaagcctgac gtgcaaattt agcagcagcg
     4321 cgcgccgcct tttccaccgt agcgcgcgtc ctgccgctca cggaaaaact cctcgtaggt
     4381 catcggcgtt tgatcgggat ggttaacccg catatgttcg acatagttgt cgtaatcagg
     4441 cataccaatc atcagcttcg ccgcctgacc taaatatttt ccggcttttg ccagtgaatc
     4501 aaacatcttg gcctccgcta accatgttgc tgaaagagtg ccggatgcgg cgtgaacgct
     4561 ttatccggcc tacaaatgct atcaacgcac tcggtcagtc ccctcgcccc atcggggaga
     4621 gggttagggt gagggggaac cctcggcact atgccaacat tcgccaacat cccccctcac
     4681 tctgacttta gtgcgcacct ttcgcctgcg ctacgatctc ctcaacattt tccggcattg
     4741 gctcatacgg cgtttctttc gccgttggct tcggctcttt caatgccgca agtgccgtct
     4801 taatcgagaa cagtgccaga accaccacga ccaccataaa gaagatggtt aacccggcat
     4861 ccagacggtt gttgaacacc agttgcgcca gctgtgactc agtatactgc gacggaatat
     4921 tgccgctgtc gatcattgcc tggaacttgt tagcaatggc caggaagccg actttcgcat
     4981 ccgggctaaa cgctttctgc cagcctgcgg tcagggtaca aatcagtagc caggccgttg
     5041 gtaccagcgc cacccaggcg taacgttgac gtttcatctt gaacaacacc acggcacaga
     5101 gcatcagcgc catccctgcc agcatctggt tggcaatacc aaacagcggc cacagagtgt
     5161 taataccgcc cagcggatcg accacgccct gatggaggaa gtagccccac gccagtacgc
     5221 acagcgccgt tgccagcagg ttagcaggca gtgaatcggt ccgcttcagg ccaagagaca
     5281 ccacgcccag cagatcctgc aacataaagc gcgcagcacg cgtacccgca tctaccgccg
     5341 tcagaataaa cagagcttca aacagaatgg cgaagtgata ccagaacgcc acatccatca
     5401 taccacccag cgcaccgtgc aggatgtaag ccatccccac cgccaacgtc ggcgcaccgc
     5461 ccgcacggga aatgatcgac tgttcaccca cttcgctggc aatctggttg agcgtatctg
     5521 gtgtaatgct aaagccccag ctactcacca cctgcgcggc agaagcgacc acatccgccg
     5581 tccctgccgg agccagcacc gccattgggc tgttcatggc aaaatacacg cccggatcga
     5641 tgatacaggc ggaaaccagc gccataatcg ccacgaagga ttccatcaac attccaccgt
     5701 agccgataaa acaagcctgc ccttcgttcg ccagcatctt cggcgtggtg ccggaagaga
     5761 tcagcgcatg gaagccagac accgcgccac aggcgatggt gataaacagg aacgggaaca
     5821 ggttaccggt ccataccggg ccagtgccat cgacaaattt ggtcagcgca ggcatggtca
     5881 gcgtcgggcg cataatcaaa atgcctaccg ccagaccgac gatcgtcccg attttcagga
     5941 aggtagagag gtagtcacgc ggggccagca gcaaccacac cggcagcacc gccgccacaa
     6001 aaccgtaacc caccagcatc caggtcagct gcacgccggt aaagtcaaag tacggtgccc
     6061 aggtcggact ttctgccacc cagccgccag agataatggc gaaaatcagg aataccagac
     6121 cgatgaccga cacttcacca atacgccccg gacgcagata gcgcaggtag atccccataa
     6181 acagcgccag cggaatggtg aacgcaacgg tatacgttcc ccacgggcta tgagtcaggg
     6241 ctttcaccac gatcatcgcc agtactgcaa ggataatgac catgatcata aagcaggcca
     6301 ccagcgctat caccccggcg gttggcccca tctcttcttt gaccagctca cccagcgatc
     6361 gaccgtcacg gcgcgtagaa acaaacagca ccatgaaatc ctgcaccgca ccggcgagaa
     6421 ccaccccggc gagcagccag atcatccctg gcaggtaacc catttgcgcc gccagtaccg
     6481 gcccaaccaa cggacctgct ccggcaatgg ccgcaaaatg gtgaccgaac agcactttct
     6541 tgtccgtcgg cacatagtcc agcccgtcgt tatggcgcac cgctggcgtc atacgcgtcg
     6601 ggtcaaccgc cagcacattt ttggcgatat acagcccata aaaacggtaa gcgatcagat
     6661 agatacagac cgacgccacc acaatccaca gcgcgttgat ctgttcccca cgatttaaag
     6721 caatgtatcc cagagcaaat gctcccatta cagagagcac tgtccagacg aggtatttcc
     6781 ctgatttgtt catagttgtt atccgtgtgc gtgtccatag atatgttaca ttttgtttct
     6841 ataacatctt tgtacgattt aacaacacgg taaccatgta aatcggagtt gaaaccaata
     6901 tttaacttaa caatgttaac tcgatcactc cgttaaccga gttttcctgc aatctcattt
     6961 aattctgtcg ggctcatccc aaaactgccg tacccagccg acaagtgcag caacgccgcc
     7021 cctgttcatc gaaaacgacg atttcccagc tctgattttg ccgcccaaga tgcagcggct
     7081 ggcagacgcc gcgtaccttg ccttcagaca ccgggcgatg atgggtggcg ttaagttccg
     7141 tacccaccac acactgcccg tcacgggtca tcataaatcc ggccatcgat cccagcgttt
     7201 cagccagcgc cgccgacgcg ccgccatgca gcaggccaaa cggctgatga gtacgggtat
     7261 caaccggcat ttcggcttcc agcacatcat ctcccagacg ggtatacaca attcccagat
     7321 gcgccaccat tgtgttatcg ctggtggcgt tcagttcgtc gagcgttaaa tggcgtttcc
     7381 agatcatgct tatgccccca gcgttgagcc gccatcgacc acaatatcct gtagggtgat
     7441 atggctggcg aggtcagagg cgaggaacaa aatcgtgttg gcgatctctt gtggacgggc
     7501 gattttcccc agcggaatgc cgagtttaaa ctgctcgcca aagccgcgaa tacgctgttc
     7561 ttcggcgtca tcgctcaccc acagcgtgcg ttgcatatcg gtgtcggtgg agccaggcga
     7621 aaccacatta cagcgcacgc cgctaaccgc cagttccagc ccgacgctca acgccaggct
     7681 tttcagcgcc gctttcgatg cgccataagc actcatacca atacgcggcg tgtgtgcggc
     7741 gtcggacgcc acagtgacaa tcgccccgcc ccgctgacgg cgaaactggt tcatggtttg
     7801 ctggaacagg ttaaacgcac cgccgacgtt aaccgcaaaa gtctgctgcc agtcctcttt
     7861 actgagctga tcggtcgcgc ccatgcgcaa aattcccgcc gcattgatca gcacgtccag
     7921 tcgctccgtt tcagctaaca gtcgctgaca tacctgcgcc acctgcccgg cgtcggcaac
     7981 atccatcact tcggtcgcaa agggatattg ctcctgagcg aacgcttgat caaaaccggt
     8041 aactttcgct cccgcctcaa caaacgccag cgccgtggcg tagccgatac ctttacctgc
     8101 gccggttacc cagacatttt taccgctgaa atccattatt tcacctcgcg ggagagtagc
     8161 ttccaccagg cgtcgatggt cgggtttttc gccagcatga caaagtcgat atcaccatgc
     8221 actttgcgcc agcgcgccgc cagcgccatc atgcgcaccg aatccagacc gtagtcgatc
     8281 aggttgtcgt catcgaacgg ttcatcggac tcgtccagca acggcaggat aacctcacgc
     8341 agcgccgctt tgctggcggg gacaggtgct ggcagtaatt cttcagtcat caccacccgg
     8401 ccagaccgcc cggccacata tttcagcgac atcaaatgct cgtcacggct gaaatcggcc
     8461 agcgcatccg ccaccataaa cggtttaata tcgcgcataa atgcgtcggt tgcggtggtc
     8521 atacagccaa tgtgggcata taccccggta ataatcagct ggttacgtcc actctctttg
     8581 agcatttgct ccagcggaga acgatgaaac gcgctgtagc gccacttcac cagcaccgtg
     8641 tcgtcggcat ctggcgtcag gcgatccacc accttttgct gttccggcga acgggtcagg
     8701 cccggccccc acatatcatt caacagcgcc cgatcttcat cgctctgctc tttcggctgg
     8761 gcggtgtaat aaaccggaat attgtgctgt ttgcagtagt cgcgcagcgc agcaatattc
     8821 gcgatcacct gctccatcat cgggcagttc tcgccccaga agctgacaaa atagtcctgc
     8881 atatcatgga ttaacaacgc ggcacgttgc ggttcaaagg cccagtcaac tttattctta
     8941 gggatatcgt gagactccgg cagtgcgtaa gcctgtaatt ttggaatagc catcgtgttc
     9001 tctccttcag gctgatgcgc gtgacgccag ccactgacgt aattgttttt tatcgacttt
     9061 cccgaccgcc gtcagcggaa gtgaatctac acactccacg cgatccggta atttaaattc
     9121 ggcaataccc tgttcacgca ggaaacgacg cacctgcacc gcgcgcagcg gctcttttac
     9181 caccagataa gcgcagcttt tttcgcccat cagctcatct tccatgctca ccagtgcggc
     9241 gtagatcacc gccgggtggc gcagcagcag gttttcgatc tcttcggcag cgatcttctc
     9301 accgccacgg ttgatctgat ctttctcgcg cccctgcacg gtgatgtacc cctctggatc
     9361 aatagagatc agatcgccgg aacagtaaaa accgttggca tcaaaggcgc tggcattgtg
     9421 ctgcgggctt ttgtaatagc cacggaaggt gtacggaccg cgggtcatca ggcgtccaac
     9481 ttccccttgc ggcagtggat ttccttcggc atcggcaacc cacacttcat cgtccggaca
     9541 cattgggtaa ccctgggtat ggataatttt ctccgcgcta tcatcaagtc gggtgtagtt
     9601 caccagccct tccgccatgc caaacacctg ctgcaactga cagccaatct cagcgggaat
     9661 acgcgccgca agggtggcag aaagacgtgc gccgccgacc tgtaacagtt tcagcgaggc
     9721 aagctgcgcc cggctttcgc cttcgatcag cgcctgcaac cacaggctga ctgcgggtgg
     9781 caccagcgcg gtaacgttaa cctgatgttt ttcaatcaat gggaaacaga gcgtggcgct
     9841 gggatcggcc gccagaacaa ccgttcctcc ggcaagaaag acgcccagcg atcccggcga
     9901 actcatggcg tagttatgag ccgccgggat cgcgcacagg tagcgtgtct gttgtgtgaa
     9961 ctgacaaatc tcgacgctac gacgcacgct gtagtagtag tcgttatgag tgcgcgggat
    10021 cagtttcggt gtgccggtgg tgccgccgga aagctggaaa taggccactt catcagcagg
    10081 tgatggcgtg gcagtaaaat cctcagccgg atggttaatc gcatcctgca agttatgctc
    10141 accgctgtcg ttgtgcagtt gcaccacgcg aatggaggaa tgttctgtga cgaaagtatt
    10201 gaggaaatca tccccgctaa acagcgcatg ttggcgatcg gcaatcagca atgcgggttc
    10261 aatctggctg gcataggcgt tcagttcact acgctgatgg ctgaacagcg ccagcaccgg
    10321 cgcaacgccc agtttcagca gcgcgaaaaa ggtaatatac aattcagcga cgttacccag
    10381 ttgtaccagc gcggtttcac caggtttaat gccctgacgg cgtaaactac acgcgaggtt
    10441 atccgccgcc tgattcagct cccggtaact caactgtcgc tcgccgtcga taaccgcgat
    10501 gctgtcactc gcagcgtggc gagtcagaat gtcggtcagc ggcaaatcct gccagtagcc
    10561 tttttcccga tagcgacggg caaactcttc cggccagcgg gtgaatggaa tgctcatcct
    10621 cgctccttaa tgcaatccaa aaacgttcaa catggtagaa agtttgacgc ctgtttcgcg
    10681 ccactcaccc aacggtgacg acgcaggcac aatccccgct ccggcaaaca gacgcacctg
    10741 attttcccgc agcttcgcgc agcggatggt caccacccat tcgccgttac cttcgctgtc
    10801 acaccaaccc acaatgccgc caaacagttc gcggtcgaat ggctccagtt cagcaataac
    10861 ctgggtcgcg gcctgatgcg ggaaaccgct cagcgcgggg gttggatgca gcagacaggc
    10921 cagagtcagt gcgttttctt gcgaattcgc tttaccttca aagggagttg cgagatgcca
    10981 cagcgtcggc gtggtaatca attgtggaga ggagggaacg tgtaactcac tactgcgttc
    11041 gcgcagtacc tctttcatcg cctgagtcac cagttcatgt tcatggcgat ctttttctga
    11101 cgccagcaga cgattacccg cttcgcgatc gagcacttca tccggctgac gacgcgcgga
    11161 accggctaac ggaatggagc taaaacgctc gccgtcttta cgtagcagca gttccgggct
    11221 ggcccccagc aggacgccac catcagccag cgggacatgg aagttgtaac taaccgggtt
    11281 ttgcgcaatc aaccgttcca gcaatacgcc actatcaatg gcggcgtcag tggtgatatc
    11341 aatcaatcgt gacaacacca ctttgtcgac ctgcggcgtg gcggtaagtg cggcggcgcg
    11401 ggcaaccatc tgttcaaacg tggtttgttc aggaattgcc tggcgttcca ccacgttcag
    11461 cgactggctg cgggtaaaac ggcgggctga ggtctgtttt tcctggcggg agaacgactg
    11521 ccaggattcg ggaatataca gcgacgaagg ctgacgtgga tcgaagggaa tcgccccgac
    11581 catcaccgga tttttgatgc cctgcgcttt ggcatcggca aacagcgcgg cgagtttttg
    11641 ctggaaggga ctgtcgggcg aatccccgtt cacagccggt tcatcgaagc gggcgaaaca
    11701 tcctgacgtc gtaaaactgc ggtacggcga cataaagaaa aagcgattgg gcgcaagtgt
    11761 tgccatggtc tgctgtactt cctcagccag tgacgtatcc atatcatcct ccacaaaatg
    11821 ataaaggctt taataatgat tatcatttat attttcggtc gctaacctaa aagcaaacgc
    11881 gcactatgtc aactcttgag gtaacgcaat ctgcaactcg gggttgcatc tggttgtggt
    11941 tacaatgaaa atgagaagca ttaacttcat taataacagg aagctgattt gtgagactcg
    12001 ccccgctcta ccgcaacgcc cttctattaa caggactttt actttcagga atagccgcag
    12061 ttcaggccgc tgactggccg cgtcagatta ctgacagccg tggcacacat acactggaaa
    12121 gccagccgca gcgtattgtt tccaccagcg tcaccctgac cggctcactg ctggcgattg
    12181 atgctccggt gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgcg gatgaccagg
    12241 gctttttacg ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg
    12301 gcgaaccgag cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa
    12361 ccggcgggga ttcggcgctg gcactgtatg atcagctttc caccatcgcc ccgacattaa
    12421 tcatcaatta cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg
    12481 ggcatgagaa acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga
    12541 aagagcaaat caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac
    12601 acagtgccaa tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct
    12661 ttacgctggc gaagttgccc gcagggctaa acgccagcca aagccagggc aaacgccatg
    12721 acatcattca gcttggtggg gaaaatctgg ctgctgggtt aaatggcgag tcactattcc
    12781 tgttcgccgg tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc
    12841 tgcccgcagt acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt
    12901 actacagcgc catgcaagtg ctggaacgac ttaaggcgct gttttaagca ttaactgtca
    12961 gacgctgtca cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat
    13021 aacaacacgc cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc
    13081 atcatcgcgc ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc
    13141 gccgtccaca aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt
    13201 gtatattgca gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg
    13261 cctaaaatcc acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg
    13321 gagagcagca tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca
    13381 ccaatagccg cgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc
    13441 tgccagttgt cagccagcgc cggatacagt acccgcaccg cgctcgccat cgtcaataaa
    13501 ccacccagca gcgcaatccc gcccaccagc gggctggcaa gcagaaaacg aaatcctgcc
    13561 agtaatgatt tcaacggatg ctcacgcggc tgcggtggcg gtggcaacgc cggaaggctt
    13621 aacaacggta gcaaggtaat aaacgtgccc gccgccgcca gcccgtagtt ccaggctacg
    13681 ccaccggtcg ctagcaataa accgccaatc atgggcgaaa tcaccgaccc cagacgcacg
    13741 gtcaacatgg tgatcgcccc ggcctgcatt aagttttcac gccctaccag tgctggtgtc
    13801 gccgccaata gcgccgtaac gccaagcgat gcgaaaaaac catcccataa accaagtaaa
    13861 tagattgcca gcaatgacgg ctccggcagc agtgcattaa ggcacagtcc aatgaagcca
    13921 atgccacagg tgccgcgcgc cagcaaaatc acttttttgc gctcatagcg atccgccagc
    13981 acaccgccaa ccatcaggcc aacaaacatc gcgccgccgg tcagcgtcac cgaaagcccc
    14041 acctgccagg tagaatgcgt catcatctgg atctgcaccg gcaccgcgac gccgagcaaa
    14101 cccagagaca caattgagat aaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt
    14161 ttcaacaggc tgaggttaag cagccaggat tgtttattca ttacaatgcc ttgccatcaa
    14221 atattttaaa tacctattct taggctgcac atgctaacat atccaaataa gatcgataac
    14281 gataattaat ttcattatca tggaagttcg tatgtctggt tctgttgccg tgacacgcgc
    14341 cattgccgtg cccggattgc tgttattact gattatcgcg acggcattaa gcctgctcat
    14401 tggggcaaaa tcactccccg cttccgtagt gctggaggcc ctctccggca cctgccagag
    14461 cgccgactgc accatcgtgc tcgacgcgcg actgccgcgc acccttgccg gtttacttgc
    14521 aggcggcgcg cttggccttg ccggggcgtt gatgcaaacc ctcacccgaa atccacttgc
    14581 cgaccccggc ttgcttggcg tgaacgccgg agccagcttt gccattgtgc tgggtgcggc
    14641 gctgtttggt tactcatccg cgcaggaaca actggcgatg gccttcgccg gggcgctggt
    14701 ggcctcgttg attgttgcct ttaccggcag ccagggcggc gggcagttaa gtccggtgcg
    14761 tttaaccctg gcgggcgtgg cgctggcggc ggtgctggaa gggctgacca gcggcatcgc
    14821 cctgcttaat cctgacgtct acgatcaact gcgtttctgg caagccggtt cgctggatat
    14881 tcgcaatcta cataccttaa aagtggtgct gatcccggtg ctgatcgccg gagcaaccgc
    14941 gctattactg agccgcgcgc tgaacagttt aagcctcggc agcgacaccg cgacggcgct
    15001 gggcagtcgc gtggcgcgca cacagttaat tggtctgctg gcgattaccg tgctttgtgg
    15061 tagtgcgaca gcggtagttg gcccgattgc ctttattggc ctgatgatgc cgcatatggc
    15121 gcgttggctg gtgggtgccg atcatcgctg gtcgctgccc gtcacgctac ttgctacccc
    15181 tgccctgctg ctgtttgccg atatcatcgg gcgggtgatt gtgcccggcg aactgcgcgt
    15241 ttctgtggtc agtgcgttta ttggtgcacc ggtgctgatc ttcctcgtgc gacgtaaaac
    15301 gcgaggtggc gcatgattta cgtctctcgc cgattactca tcacctgttt gctgctgatt
    15361 tccgcctgtg tggttgcagg tatctgggga ttacgcagcg gtgccgtcac gctggaaatc
    15421 tcgcaggtat tcgccgcgtt gatgggcgat gcgccgcgca gtatgacgat ggtggtcacc
    15481 gaatggcgtt taccacgcgt gctgatggcg ctgttgattg gcgcggcact gggcgtcagc
    15541 ggtgcgattt ttcagtcgct gatgcgtaac ccgctcggca gccctgacgt aatgggcttt
    15601 aacaccgggg cgtggagcgg cgtgctggtg gcgatggtgc tgtttggtca ggacctgacg
    15661 gctatcgcgc tgtcagcaat ggtgggcggc attgtcactt cgcttctggt ctggctgctc
    15721 gcctggcgta acggcatcga cacctttcgg ttgattatta tcggtatcgg tgttcgcgcc
    15781 atgctggtgg cctttaatac ctggctgttg ctgaaagcgt ctttagaaac ggcgctaaca
    15841 gcgggtttgt ggaatgccgg atcgctcaac ggcctgacgt gggcaaaaac ctcgccttcc
    15901 gcacccatca ttatattgat gctcattgcc gccaccttgc tggtgcggcg aatgcgcttg
    15961 ctggagatgg gcgatgatac cgcctgcgcg ctgggcgtca gcgtcgaacg ttcgcgtctg
    16021 ttaatgatgc tggttgcagt ggtgcttacc gctgcggcaa cggcgcttgc cgggccgatt
    16081 tcctttattg ctttagtcgc gccgcacatt gcccgacgca ttagcggcac cgctcgctgg
    16141 gggctaaccc aggcggcgct gtgcggggcg ctgttactgc tggcggccga tctctgcgcc
    16201 caacaactgt ttatgccgta tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt
    16261 atttacctta tcgtcttgtt aattcaggag tctcgcaaaa aatgaccgaa tcagtagccc
    16321 gtttgcgcgg cgaacagtta accctgggat atggcaaata taccgttgcg gaaaatctga
    16381 ctgtagaaat tcctgatggt cacttcacgg caattatcgg accaaatggc tgtggtaaat
    16441 ccacgttact gcgtacctta agccgcctga tgacgcctgc tcacgggcat gtctggctgg
    16501 atggcgagca cattcaacat tacgccagta aagaagttgc acgccggatt ggtctgttgg
    16561 cgcaaaatgc taccacgccg ggcgatatca ccgtgcagga gctggtggcg cgtggacgtt
    16621 atccgcatca accgctgttt acccgctggc gcaaagagga tgaagaagcg gtaacgaaag
    16681 cgatgcaggc cacgggaata actcatctgg cagatcaaag cgtggatacc ctttctggcg
    16741 gacaacgcca gcgagcgtgg atcgcgatgg tgctggccca ggaaacggca attatgctgc
    16801 tcgacgaacc cacgacctgg ctggatatca gtcatcagat tgatctactg gaactgttaa
    16861 gcgaactgaa ccgcgagaaa ggctataccc tggcggcggt gctgcacgat cttaatcagg
    16921 cctgtcgtta cgccagccat ttgattgcat tgcgggaagg gaaaattgtt gctcagggag
    16981 cgccgaagga gattgtcact gctgaactga ttgagcgcat ttatggtctg cgatgcatga
    17041 tcattgacga tccggtagcc ggaacgccgc ttgtggtgcc gctcggacga acggcacctt
    17101 caaccgcaaa tagttaaact aagtggtctg ccatcatggc atcctgtttt ctggatgcca
    17161 tcgcatagcg caataacacg ctaccacaag ccaccatccc gccgatcagc gcggaaagga
    17221 tcacaataat cgccttaccc ggaccgtctt ttttcactgg caatgacggg cttaactgat
    17281 atttaaacgg cgtaaaattc acatcgttga tatttgcttt tgttaactgc tcaacaagat
    17341 actgacgatt acgtaattca ccgttcagtt ccgcaacgtc agtgaccgct ttttcaattt
    17401 ccagtttgcg ttcaataccg tctgcgccga gagaaataga gaaatcgggg tcatctttaa
    17461 cggcctgacc attactgtat acgggctttt taattcccgc cgcgttggca atgtcgagtg
    17521 aataattgag gcgctgaatg tttgcatcaa gttgattttt cgttttaatg cgatcctgag
    17581 ccagtttttc tttttcaaac tgggttttga tctccagttt attacggacg ttttctatcg
    17641 actctttcac caccaacgca gagatataat cgatataccc gctcaaaacg gtctgcgcct
    17701 ctttactggt tggcgcggta aaacttagcg tccaggaggt atacagtgac ggttcatctt
    17761 ttttcttact ggcattgtca tcaaccgctt tcattttttc gctcaatgca acaatagcgc
    17821 gatgcaaatc cagttcatcg attttcgcct cttttaattg gtccatcaca taaggtgatg
    17881 aacgcaggta ctcttccagc aagctaacag actgaaactt cttgataaac aggttaaatg
    17941 caccgccacg gtcgatatta acatcgagat caagaacgcg gagtttggta aaggttttct
    18001 ccaggtcctg ccactgaatg gcttcggcgg gagtaataac ggctgagctg gtccattttt
    18061 gcggcaggat gaaagagatc agtaaacctg cgcaggcaaa tgcaaaaacg accgccatga
    18121 ccgttttttt ggcccgccat aaaacttcga ttagattaag taaatcaatt tcattattac
    18181 tgggcgacgc cagaggatac tcagaaaaat gagcgtcact tccctgttta atattcaatg
    18241 atgacatggg gtataacctg aaaatgagtc caatagggga aaatctccac ggcgctcact
    18301 ttaccagttg gtgagaatta tccgaagctg aagtttgtaa attccttcca ctgaatgcaa
    18361 cattcactct atttaagaga aataactagc aaacttataa actaaataaa tgattaaccc
    18421 ataattattt cctataatta tgggttaata gataatgtta atttacctgt ttagcgttgc
    18481 gcgaataatc ggcccaattt tttcaaacgc ccctggagag ataatatcca catgcgcaca
    18541 atcctgacga tagatatcca gctccgcaat ccacggcgac caggcgcgtt cggggctcat
    18601 accttcctga agcgtgcgtt cagcaacaaa cagcgtcgct ttaccatcaa acggtacgct
    18661 atgagcagtc gtcagcaggc gcacagcatc agcgtagttg ccttcaatgg tggtaaacaa
    18721 ctccgttgaa gtacttccct gctgtgccgc caggaaggcc tcgcgctcgc ggttaatctc
    18781 cgccagcact tccgggtcca gaccattagc ttctttttcc tgccagtttt gcgtttctgg
    18841 cggccaggta tccagcaagc caagaaatgc cacctgttcg ccacgggcac gcagtcgcgc
    18901 cgcaataccc tgcaccagcg taccgccaag ggaatacccc agcaggtaat aagggccgtg
    18961 cggttgttgc tcaagtaacg ttgccagatg cgcttcgcag acttcatcca ggtttgtcgc
    19021 tgtctgcatg ggtccatgag ggcgcggcga ctgaatgcca ataatcgacc attgtggatc
    19081 gagataacgc gagagcacgc taaactgcca ggcaaaaccg gatgccggat ggaaacaaaa
    19141 cagcgtcggg ccattacctt cacgcaacgg cagaatggtt tcgaatccca tgcgccggga
    19201 gctgtcctct tcaccatcaa taatcgttgc cagtttggcg acggttgacg cgaccatcac
    19261 ctgccccggc gtcacctgac gagcaaactg tcgacttaac tgcgctgcca gtttcattgc
    19321 cagtagcgaa tgaccgccca gcgcaaagaa atcagcatcg gcatcctgca cgtcacaacc
    19381 cagcaacgac gcgaatgccg cggcgataat cttttcactg cccgctttcg gtgcacgtcc
    19441 cgacgcttgt gtcttcagtt caggcaacgg taaggctttg cgatccagct tgccgttggc
    19501 gctaagaggt aactgtggaa gttgcagcag aaccaccggc accatatgcg gcggcaatgt
    19561 ttcgcgaagc tgtgcctgta atgcgctggt atccaacggc agacctgatt gcgatacaag
    19621 atagcccacc aactgacgcg catcaccacc agtggctgcc gccaggttaa tcacacaggc
    19681 gtgggtaacg gcttgttcga catccggcag cgcctgcatc acgcgatcga tttcgcccag
    19741 ttcaatacgc tgcccgcgaa tttttagctg atcgtcactg cgcccgaggt actccaccgc
    19801 gccgttatcc agccagcggg caacgtctcc ggtacggtac atccgttcac caggagcaaa
    19861 aggatcggca ataaagcggc tggcggtcag atcgggtcgt ccaagatacc cctgcgccag
    19921 ttgaataccg gtgagataga gatctcccgc cacacccggc ggcaccggat gcatcatcgc
    19981 atcaagaata cgcaggcccg tgttccacac cggataacca atcggcacac tgctgccgcg
    20041 cacctgtgcc agttcctcgc caaaagccgg ataccagctg acatctaccg ccgcttccgt
    20101 cgggccatac agattatgca acggcgcgcc ggttaactgt tgccattcgc ggcataaatc
    20161 agccggtaag gcctcaccac tacagaaaac ctgtttcaac gtcgcgcaat tctggcgagc
    20221 ggtttgcggc gtcagcgatg caacaaatgc cgccagcatc gacggcacaa agtgcgtggt
    20281 cgttacgcca tattcggcaa agaattgctg catagcgagc gggtcacggt gcgcttccgg
    20341 ttcagccatc accagttttg cccctgcgat aaacggccag aaaaactccc acaccgagac
    20401 atcaaaactg cacggcgttt tttgggcaac gacatcttcg cctgtaagcg ggtagtgatt
    20461 ttgcatccat aacaggcggt taacgatagc cgtctgcccg accattaccc ctttcggcct
    20521 gccggtggag ccagaggtaa agatgatata agccgtgtgg tgcggttgtg aaagttgcag
    20581 cggcgcactg ccctgggggg taagcggggc gttatagcaa aggcttgtta aattgggaac
    20641 atcgctaaag cgcggcagtt gatcgtcggt ggtaattaac agcgacggac gcgcatcttc
    20701 cagcatcatt ttcaggcgat cgtccggata gccggtatcc agcggtagcc aggccgcgcc
    20761 agcttcaact attgcatgga gtgccagggt caaaaagacc gagcgcggta gtgccaccgc
    20821 cacgctgtcc cctggtttaa cgccgcgctc acgcagcaga ttcgccagcg ccaccacctg
    20881 ctcgcgcatt tcccgatagc tgaacaggta acgcgcatct gccagcgccg gagcatccgg
    20941 tgtttttgcc gcttgttctg ccaccagcgc gctcagcgtg gtttctggaa tctcaacctg
    21001 agtggcgttg atctgcgcca gctgcgcata ctcacctggc agcataatat cgacatcgcc
    21061 gcacaacagc gccggatccg cggcgaactg ggcaatcagc attttcaggc gttcagcatg
    21121 ctggattaac gttggctcat cgtaacgctg tttattggcg aggatctcaa tactcaaatc
    21181 accgtgttca tccgggaaca gggccagttc aaggtcatta accggaccgg ttgccagggt
    21241 atgggtttgc gtctgaacac caggaatatc cagttggtaa tcaaatacct taatattgag
    21301 taccggacca aacagcggtt cctctcctgc cgctcgcccg ctgtcacgga caatttgttc
    21361 ggcatcgtaa cgttgatgac gacgcatttt tttcagttgt gctgccagtc gggttgccag
    21421 ttccggcagc gtttcttgcg ccgcaatgtg aatacccaac ggtaaaacgt tgagcacggg
    21481 tccgctagcc gtcagcgccg ccgagcccag tcgacgcata aagataaatc cggcggcata
    21541 gtccatgcga ttgcacaatc gccccagcca caaggctgcc agcgcaaggg ctaaatcggt
    21601 acgctgcaca cctgaaagtt gcgtagccag ctggcggaat tccccgtcgg taaattccag
    21661 tttcaggcgc agaatatctg ccgaagcgct gcgccccgct aaaggtgccg gagaaagtga
    21721 cgcgggcggc ggcagttgac gacgctgttc tgcccagaat gccgcatcac gctgccaggc
    21781 ttcgctttcg cggtattgct ggtactcttc cactacatca gcgaaaggcg taaacggcga
    21841 agcaggcgtt ggttcgccac gcagccatgt gcagtaaata ttggcgatct ggcgagtaat
    21901 ggccgggaaa ctgaagccat cgaccagcaa atggtgataa cgctgatacc agtaccagcg
    21961 gttatccgca acatgtatca gctgatgaaa gaccagtggt ttaccgctat cgacgcgcaa
    22021 atcttgttgc aaatccgcca gcattaatgc ctgcgcagta ccgtgcggat caatattggt
    22081 tcgcaggtcg ataatttctg gcagttcgaa tatcagcgca tcatcgaccc actgccagac
    22141 ttcgccgtta tcttccgtaa aacgcatccg caacgtatct gcttgcgcta gtccgacaac
    22201 caccgcgcgg gccagtaatg gcgcatcaac atctccagtt aactcaacgt aatgcgccac
    22261 gctccaggcg gagggtaaat ccgacagttt ttctgccatc cagatgccgg gctgtgcggc
    22321 gaccaaaggt aaatgctggc tcattgtgcc tcctgcaact gggtgaaatt ggccggtgtc
    22381 agagtacgcc agtggacttc cagccactgc tggcaggacg cctgtgactg cggctgacac
    22441 acaatgtccc agcctgccgg taaggcgcat tgttgcggcc acagactgaa ttgcccctgc
    22501 gcattgcgca aaatgtaaaa cgctccctgt ggatcatcga agggattact gaatgccata
    22561 ttcaactcct gtcatggaaa agtggttgcc agaggtcgat tagcccctgc atcaagccac
    22621 cgcgccaaca aagcgcatca tgtccgccgt caacctgacg ccagaaaatg gattctttta
    22681 tggggtgtaa ttgcgcatac agcgcctgat tggcccgcat aatcatcggc tcgcgaatac
    22741 ccgcttccag cacaatgcgc agaccttcgg cgctaacttc accagcttta agcttttcaa
    22801 gtaacacgcc ctcttgctgc ccgccccgat gcggccacca gtacgatcct gactggctta
    22861 atacacagcc aaagcgttca ggccagtgca gtccggcata cagcgcggac agcccaccaa
    22921 aactctgccc ggcgaccacg gtgcgatcgg cgcgatcgct aaaaggggca atagctttca
    22981 ccaggggtaa taactcttgc tgtactgcca gccagaaatc cgcattacac ggcagttcgt
    23041 gggcgcggtg cgtggtgtcg atagcgtcga tcaacacata cacggcggga ggaagttgct
    23101 gacgatgggt cagcgaagtc agcacaggcc agacgggcat actttgcgcc caaaattcgc
    23161 catcgagcaa aactgccagc ggacgttctt ctgctgttgc atcgccggtg gtaaaaatcc
    23221 atacacgacg tgaatttttc aaccgttcac ttttccagat aatttctttg gctgctattt
    23281 ctggcgcttg cggacaatcc catcccggtt gcagaggcgc ttgcggcatt tcgagtgcag
    23341 aaacagcgtg ccctcgcccg cctttccagc tttgtggatt cagcggatcg gctattgcct
    23401 ggggtaatag ttttcgccag ccttcacgta attcgaggcg atcgggggat ggtgcagaaa
    23461 aaatgtcatc gcgttcggtg ggaataaagc agtagctgcc gcgccagttg gcattgagtt
    23521 gtgtcgtcca ctgccagaca tcagtacctg caattcgctg catcgactgg ggctggctgt
    23581 tctgatgatg atcggtcaca ccagtgatgt agatccatac gcgctttatc gtcgagtatt
    23641 cttcagaacc ttggggatca cgccaccaga aagtgacctc aaacatttcg tcattcagac
    23701 gctgccattc cgggccatgt ttcgactgcc accagctctc acttcctact tttaacgccg
    23761 tcacaccata accccatgtt tactgtgcaa tttttcattg attgcagaaa tatattgata
    23821 atattattga taactatttg catttgcaat agcgtaatgg cgcgccgtgg gaagcgcgga
    23881 catttattaa ccaactgcac tgcgtgtctt tcaggatcaa aggttttcgc ggtagcggga
    23941 tgcgtcgtgt tgaggacgac catgcccgac agttgcaatt cgtggcaaaa atgcaggaat
    24001 aaaacaatga acaagaagat tcattccctg gccttgttgg tcaatctggg gatttatggg
    24061 gtagcgcagg cacaagagcc gaccgatact cctgtttcac atgacgatac cattgtcgtt
    24121 accgccgccg agcagaactt acaggcgcct ggcgtttcga ccatcaccgc agatgaaatc
    24181 cgcaaaaacc ccgttgcccg cgatgtgtcg gagatcatcc gtaccatgcc aggcgttaac
    24241 ctgaccggta actccaccag tggtcagcgt gggaataacc gacagattga tattcgcggt
    24301 atgggtccgg aaaacacgct gattttgatt gacggcaagc cggtaagcag ccgtaactcg
    24361 gtgcgtcagg gctggcgtgg cgagcgcgat acccgtggtg atacctcctg ggtgccacct
    24421 gaaatgattg aacgtattga agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc
    24481 gcggcgggcg gcgtggttaa catcattacc aaaaaaggca gcggtgagtg gcacggctcc
    24541 tgggacgctt atttcaatgc accagaacat aaagaggaag gtgccactaa acgcaccaac
    24601 ttcagcctga ccggtccgct gggcgacgaa ttcagcttcc gcttgtatgg caacctcgac
    24661 aaaacccagg ctgacgcgtg ggatatcaac cagggccatc agtccgcgcg tgccggaacg
    24721 tatgccacga cgttaccagc cgggcgcgaa ggggtgatca ataaagatat taacggcgtg
    24781 gtgcgctggg acttcgcacc attgcaatcg ctggaactgg aagcgggtta cagccgccag
    24841 ggtaacctgt atgcgggcga tacccagaac accaactctg acgcttacac tcgctcgaaa
    24901 tatggcgatg aaaccaaccg cctgtatcgc cagaactacg cgctgacctg gaacggtggc
    24961 tgggataacg gcgtgaccac cagcaactgg gtgcagtatg aacacacccg taactcgcgt
    25021 attccggaag gtctggcggg cggtaccgaa gggaaattta acgaaaaagc gacacaggat
    25081 ttcgtcgata tcgatcttga tgacgtgatg ctgcacagcg aagttaacct gccgattgat
    25141 ttcctcgtta accagacgct gacgctgggt acggagtgga atcagcaacg gatgaaggac
    25201 ttgagttcca atacccaggc gctgaccggg gcgaacaccg gcggcgctat tgatggtgtg
    25261 agtgccaccg accgtagccc gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac
    25321 aacatggagc tgactgacag caccatcgta acgccggggc tgcgtttcga tcatcacagt
    25381 attgtcggca atcactggag cccggcgctg aacatatcgc aaggtttagg cgatgacttc
    25441 acgctcaaaa tgggcattgc ccgtgcttat aaagcaccga gcctgtacca gactaacccg
    25501 aactacattc tctacagtaa aggtcagggc tgctatgcca gcgcgggcgg ctgctatctg
    25561 caaggtaacg atgacctgaa agcagaaacc agcatcaaca aagagattgg tctggagttc
    25621 aaacgcgacg gctggctggc gggcgtcacc tggttccgta acgattatcg caataagatt
    25681 gaagcgggct atgtggctgt agggcaaaac gcagtcggca ccgatctcta tcagtgggat
    25741 aacgtgccga aagcagtggt tgaaggtctg gaaggatcgt taaacgtacc agttagcgaa
    25801 acggtgatgt ggaccaataa catcacttat atgctgaaga gtgaaaacaa aaccacgggc
    25861 gaccgtttgt cgatcatccc ggagtacacg ttgaactcaa cgctgagctg gcaggcgcgg
    25921 gaagatttgt cgatgcaaac gaccttcacc tggtacggta agcagcagcc gaaaaaatac
    25981 aactacaaag gtcagccagc ggttggcccg gaaaccaaag aaatcagtcc ttacagcatt
    26041 gtgggtctga gcgcgacctg ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac
    26101 aacctgttcg acaaacgttt gtggcgtgcg ggtaatgccc agaccacggg cgatctggca
    26161 ggggccaact atatcgcggg tgccggggcg tatacctata acgagccggg acgtacgtgg
    26221 tatatgagcg taaacaccca cttctgatgc taacgtcaga ttgttgacaa agtgcgcgtc
    26281 gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaaaatcttg ccaattcaat
    26341 atattgcagg accatgttgg cctgataagc gtagcgcatc aggcagtttt gcgtttgtca
    26401 tcagtctcga atatggtcga tatgaaaact acgcatacct ccctcccctt tgccggacat
    26461 acgctgcatt ttgttgagtt cgatccggcg aatttttgtg agcaggattt actctggctg
    26521 ccgcactacg cacaactgca acacgctgga cgtaaacgta aaacagagca tttagccgga
    26581 cggatcgctg ctgtttatgc tttgcgggaa tatggctata aatgtgtgcc cgcaatcggc
    26641 gagctacgcc aaccggtgtg gcctgcggag gtatacggca gtattagcca ctgtgggact
    26701 acggcattgg ccgtggtttc ccgtcaaccg attggcgttg atatcgaaga aattttttct
    26761 gcgcaaacag cgaccgaatt gacagacaac attattacac cagcagaaca cgagcgactc
    26821 gcagactgcg gtttagcctt ttctctggcg ctgacactgg cattttccgc caaagagagc
    26881 gcatttaagg caagtgagat ccaaactgat gcaggttttc tggactatca gataattagc
    26941 tggaataaac agcaggtcat cattcatcgt gagaatgaga tgtttgctgt gcactggcag
    27001 ataaaagaaa aaatagtcat aactctgtgc caacacgatt aattgacaac atctggtacg
    27061 attcgcccgc agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc ttaccactct
    27121 gacctgacaa ccggatatgc ggggattgct ccccgcacaa cggctacttc ttcggttcgt
    27181 aagcgagaac agccttaaac tcaatattac gttcctttac ggtgaactca cacagcgagt
    27241 ctccgaccag aagcgtaaat cccagtaccg tcaaacacag cactatgact gccacaaggg
    27301 catatttcgt cagcatgttt atattgcctc catgcgaaga agggactacc atccgagttg
    27361 ttgaggctcg gagtggcagc cccaggattg atagaaatat ctcctggggc ttcatctttc
    27421 cagacctcag agttaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt
    27481 cctcaacaaa atagctgttt atttatacag taaatcctgt ttcggacttc tgtcggcaaa
    27541 actcatcatt gctcttaaaa cgaaatcgcc cgcagccatc actgaccgcg ggcgaaagtg
    27601 taaagcaggt gccttaccat cctgacctga caaccggata tgcggggatt gctccccgca
    27661 caacggctac ttcttcggtt cgtaagcgag aacaacctta aactcaatat tacgttcctt
    27721 tacggtgaac tcacacagcg aatccccgac cagaagcgta aatcccagca ccgttaaaca
    27781 cagcactatg actgccgcaa gggcatattt cgtcagcatg tttatattgc ctccatgcga
    27841 agaagggact accatccaaa ttgttcaggt ttgggtaggt agccccaggg ttgatagaaa
    27901 tatcgtcctg gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca
    27961 ggcacccgca gttatcttac ccttgcttag caaaatagct gtttatttat acagtaagtc
    28021 ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt tttttctgca
    28081 tgcctcaatc gtcaaaattc ttcgccacca gccagccgtg ccataacttg tcacattgca
    28141 aaagtagcca aattgcttca gtcaccatca ccaaaaaaag aaacattcca gaacttccgc
    28201 atctcgccca gccgctccga cttctatact gaatagaaaa cgccaactta agagaaacct
    28261 atgccattac ccgattttca tgtttctgaa ccttttaccc tcggtattga actggaaatg
    28321 caggtggtta atccgccggg ctatgactta agccaggact cttcaatgct gattgacgcg
    28381 gttaaaaatg agatcacggc cggagaggta aagcacgata tcaccgaaag tatgctggag
    28441 ctggcgacgg atgtttgccg tgatatcaac caggctgccg ggcagttttc agcgatgcag
    28501 aaagtcgtat tgcaggcagc cgcagaccat catctggaaa tttgcggcgg tggcacgcac
    28561 ccgtttcaga aatggcagcg tcaggaggta tgcgaaaacg aacgctatca acgaacgctg
    28621 gaaaactttg gctatctcat ccagcaggcg accgtttttg gtcagcatgt ccatgttggc
    28681 tgcgccagtg gcgatgacgc catttatttg ctgcacggcc tgtcacggtt tgtgccgcac
    28741 ttcatcgccc tttccgccgc gtcgccgtat atgcagggaa cggatacgcg ttttgcctcc
    28801 tcacgaccga atattttttc cgcctttccc gataatggcc cgatgccgtg ggtcagtaac
    28861 tggcaacaat ttgaagccct gtttcgctgc ctgagttaca ccacgatgat cgacagcatt
    28921 aaagatctgc actgggatat tcgccccagt cctcattttg gcacggtgga ggttcgggtg
    28981 atggataccc cgttaaccct tagccacgcg gtaaatatgg cgggattaat tcaggccacc
    29041 gcccactggt tactgacgga acgcccgttt aaacatcagg agaaagatta cctgctgtat
    29101 aaattcaacc gtttccaggc ctgccgctat gggctggaag gcgtcattac cgatccgcac
    29161 actggcgatc gtcgaccgct aacggaagat accttgcgat tgctggaaaa aatcgcccct
    29221 tccgcacata aaatgggtgc atcgagcgcg attgaggccc tgcatcgcca ggtcgtcagc
    29281 ggtctgaatg aagcgcagct gatgcgcgat ttcgtcgccg atggtggctc gctgattggg
    29341 ctggtgaaaa agcattgtga gatctgggcc ggtgactaag tagggaattg ccttacagaa
    29401 gaatatccct gacaatggtg ttttaactta catttaacaa tgttatgaaa caccctttag
    29461 aaaccttgac taccgcagca ggcattttgc tgatggcttt cctctcttgc ctgctgctgc
    29521 ccgcccccgc actggggctg acgctggcac aaaaactggt gaccatgttc catctgatgg
    29581 atcttagcca gctttacact ttattgtttt gtctgtggtt tttagtgctg ggcgctattg
    29641 agtattttgt tctgcgcttt atctggcgac gctggttctc gctggcggat taaacgtcga
    29701 ttatcgccgt acttcgcggc atactttgct tattctcctt cgcctaaagg aacgtttatg
    29761 gataagcaat cactgcacga aacggcgaaa cgcctggccc ttgagttacc ctttgtcgag
    29821 ctttgctggc cttttggccc ggagttcgat gtttttaaaa ttggcggcaa aatttttatg
    29881 ctgtcgtcgg agctgcgcgg cgtccccttt atcaatctga agtccgatcc acaaaaatcc
    29941 ctgttaaatc agcagatata cccgagcatt aagccagggt atcacatgaa taaaaagcac
    30001 tggatttcgg tgtatcccgg cgaggaaatc tccgaagcgt tacttcgcga tctgatcaac
    30061 gattcgtgga atctggtggt tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca
    30121 ggctaaagcg gaaatctata gcgcattttt ctcgcttacc atttctcgtt gaaccttgta
    30181 atctgctggc acgcaaaatt actttcacat ggagtcttta tggatatcat ttctgtcgcc
    30241 ttaaagcgtc attccactaa ggcatttgat gccagtaaaa aacttacccc ggaacaggcc
    30301 gagcagatca aaactctcct gcaatacagc ccatccagca ccaactccca gccgtggcat
    30361 tttattgttg ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat
    30421 tatgtgttca acgaacgtaa aatacttgat gcctcgcacg tcgtagtgtt ctgtgcaaaa
    30481 accgcgatgg acgatgcctg gctgaagctg gttgttgacc aggaagatgc tgatggccgc
    30541 tttgccacgc cggaagcgaa agccgcgaac gataaaggtc gcaagttctt cgctgatatg
    30601 caccgtaaag atctgcatga tgatgcagag tggatggcaa aacaggttta tctcaacgtc
    30661 ggtaacttcc tgctcggcgt ggcggcgctg ggtctggacg cggtacccat cgaaggtttt
    30721 gacgccgcca tcctcgatgc agaatttggt ctgaaagaga aaggctacac cagtctggtg
    30781 gttgttccgg taggtcatca cagcgttgaa gattttaacg ctacgctgcc gaaatctcgt
    30841 ctgccgcaaa acataacctt aaccgaagtg taattctctc ttgccgggca tctgcccggc
    30901 tatttcccct cagattctcc tgatttgcat aaccctgttt cagccgtcat cataggctgc
    30961 tgttgtataa aggagacgtt atgcaggatt taatatccca ggttgaagat ttagcgggta
    31021 ttgagatcga tcacaccacc tcgatggtga tgattttcgg tattattttt ctgaccgccg
    31081 tcgtggtgca tattattttg cattgggtgg tactgcggac cttcgaaaaa cgtgccatcg
    31141 ccagttcacg gctttggttg caaatcatta cccagaataa actcttccac cgtttagctt
    31201 ttaccctgca ggggattatc gtcaatattc aggcggtatt ctggctgcaa aaaggcaccg
    31261 aagcggcaga tattctgact acctgcgcgc agttgtggat catgatgtat gcgctgcttt
    31321 cagtcttctc gttgctggat gttattttga atctggcgca gaaattcccg gcggcatctc
    31381 agttaccgct gaaagggata tttcagggga ttaaactgat cggcgcaatc ctggtcggca
    31441 ttttgatgat ctcgctgctg attggtcagt cgccagcgat tctgatcagc ggtcttggtg
    31501 caatggctgc cgtgctgatg ttggttttta aagatccgat tcttggtctg gtggcaggta
    31561 ttcagctttc cgcgaacgat atgctgaaac tgggcgactg gctggagatg ccgaaatacg
    31621 gcgcggatgg cgcggtgatc gatattgggt taaccaccgt caaagtgcgt aactgggaca
    31681 ataccattac cactattccc acctggtctc tggtttctga ctcctttaaa aactggagcg
    31741 ggatgtcagc atctggcggg cgacgtatta agcgcagtat cagtattgat gtcaccagta
    31801 ttcgttttct tgatgaagac gaaatgcaac gtctgaataa agcgcacttg ttaaaacctt
    31861 atttaaccag ccgccatcag gaaattaatg agtggaatcg ccagcaaggt tctacggagt
    31921 cgatattaaa cctgcgccga atgaccaata ttggaacctt tcgtgcctat ctgaacgaat
    31981 atctgcgtaa ccatccgcgg attcgtaaag atatgacctt aatggtacgc caactggctc
    32041 caggtgataa cggtttaccg ctcgagatct acgcgtttac taacaccgtg gtgtggctgg
    32101 aatatgaaag cattcaggct gatatattcg atcacatatt tgcgattgtc gaagagtttg
    32161 gtctgcgact tcatcagtcg ccaaccggca atgatattcg ctctctggcg ggtgcattta
    32221 agcagtaatt aaaaaaaccg ctctcatcga atggatgaga gcggacatcg ttttgtcgga
    32281 tgtagcgtga atgccttatt tccgacgcag cgttttaaat gccacaaaca ggaatacaat
    32341 ccacaccggc agcaggatcg ctgacaagcg catatcatcc atcgtgcaca tcagcagcaa
    32401 aatcatgccg aggaaggcaa tgcaaagata gttgccgaac ggatagagca gcgccttaaa
    32461 ctgtgtttca cgcccctgac gtcgcatcgc tgcacgaaaa cgcagatgcg ccagacagat
    32521 cataatccag ttcaacagca gcgttgctac caccagcgcc atcagcagac caaacgcttt
    32581 ttgcggcagc agatagttga ttaataccac cagcgaagtg atcgctccgg aaagcatcag
    32641 cgagttaatc ggcacaccgc gacggctgac gcgagtcaaa aacttcggcg cattaccctg
    32701 cacagaaagg ccaaacagca tgcggctgtt agagtaaacc ccgctgttat acactgacag
    32761 cgatgctacc agaatgacga agttcagcgc agaagctacc acgttgctgt cgagattatg
    32821 gaaaatcatc acaaacgggc tactgttgga tttcacttcc acccacggat agagcgccag
    32881 taaaaccacc agtgaaccga tgtaaaacag caggatgcga tacaccacct gatttaccgc
    32941 ttttggaatg cttttttccg gatcgcgcgc ttcagcggca gtaatcccaa tcagctccag
    33001 accgccgaag gagaacataa ttaccgccag cgacaaaatc agcccattcc agccggtggc
    33061 aaagaaacca ccgtagcgcc agaggttgtc gatactggct ttctcgccgc cgtgaccaga
    33121 aaacagcagc cacaggccaa agccgatcat accgatgatc gccagcactt taatcagcgc
    33181 aaaccagaac tcggtttcac catataagcg cacgttcacc aggttaacgg cgttgatgat
    33241 aataaagaag gcggcagccc aaatccacgt tggaacatcc gggaaccagt actgcatata
    33301 gatgcccgca gcggtcagct ctgccattcc caccagcacg aacattaccc agtagttcca
    33361 gccagagagg aagcccgcaa acggtcccca gtatttatag gcaaagtggg caaatgaacc
    33421 ggataccggc tcttcgacca ccatctcgcc aagctggcgc ataatcagga aagcgatgat
    33481 cccggcgacg ccgtagccca gcaatacagc cggacccgcc atctgaatcg ccgggccaat
    33541 gccaagaaac agaccagtac caattgcgcc acccagcgca atcagttgaa tatgacggtt
    33601 atgtaatccg cgatgaagcg tcggctcttg attcgacgca gtatcttccg atacggttga
    33661 cgcgtttttc acgcctttcc cctgtgtgtc ttttttgttg aggggcacct tttaacattt
    33721 agtgcccatc gtcgcaagac acaatccaca cggttaaacg gggtatcctg cttttatttc
    33781 cgtacccgat gtcggtgcag ccacatcagc ttatacgccg ccgggataat aaacagcgac
    33841 agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg caatccggct catcacctct
    33901 gaaccagccc ccgttcccca cagaatcggc agcagaccgg cgataatcac cgccaccgtc
    33961 atcgctttcg ggcgcacgcg caggaccgcg ccgtgatata acgcctcatc cagcttctgc
    34021 tcgctgaatg tttgtggatt attcaacgac ggcacggcct ctatggcgtg acgtaaatac
    34081 atcagcatca ccacgccaaa ttcggcggcg accccggcga gggcgataaa gccagtgccc
    34141 gtcgccacgg aaagatgaaa gcccatccac cacagcaacc agatgccgcc caccagcgca
    34201 aacggtacgc tgctgataat cagcaacgct tcgcccaccc gacggaacgc cagatacaac
    34261 agcacgaaga taatcatcaa cgtcatcggc accatgagtt taagcttatg gttggcgcgc
    34321 tccagtagct cgaactgccc ggagaatgcc acgctggtgc ccggttttaa ctgcactttt
    34381 tcagctatcg ctttttgcaa atcgtgaacc accgacacca tgtcacgatc gcgggcatcg
    34441 atataaatcc agctcgtcgg gcgcgcattc tcggttttca gcatcgacgg tccggtagag
    34501 actttaatgt cggccacgtc tgccagggtg atttgctgct tcatcggcgt caggatcggc
    34561 agctggcgca gtgcctgcgg actatcgcgc cagctttgcg gataacgcag attaattgga
    34621 taacgggcaa tcccttccac cgtttcgcca accatcgccc cgcccaccgc agaagtcaca
    34681 aacaactgca catccgccac cgtcataccg taacgtgcgg ctttttcacg gttaatctca
    34741 acgttgatat agcgcccacc ttccagccgc tcggcaagcg cagaagctac gcctggcacc
    34801 gttcgcgcta cttcttcaat ttgctcagcc atcgcgtcga tatccgccag cacagtgccg
    34861 gaaactttaa tgccgatggg gcttttaatg ccggttgaga gcatatcgat acggttacga
    34921 attggcggca cccacagatt cgccagcccc ggcagccgca cggtgttatc cagttcctca
    34981 atgattttgt ccatcgtcat gcctggccgc cactgctcct gcggcttaag ctggatggtc
    35041 gtttctacca tctccagcgg agcagaatcg gtggcggttt ccgctttccc ggttttgcca
    35101 aatacccgcg ccacttcagg tacgctcata attagcttgt cggttttttg cagcatactc
    35161 gccgcctctg ctgcggaaat ccccggcagc gtcgatggca tatacaacaa gtcgccttca
    35221 ttgatctgcg gtaaaaattc cccgccaact ttattgagcg gccagagaac cgtcagcacc
    35281 gaaagcgccg ccaccagcag cgtggttttc ggccagtgca gtactttcag caacagcgga
    35341 tgataaacac gaatcaaaaa gcgattgagc gggttactgc tttccggcgg aattttgcca
    35401 cggatccagt agcccatcag gatcgggatc actacgatcg ccagcagcgc cgcacccgcc
    35461 atcgcatacg ttttggtgaa cgccaacggg ccaaacagac gcccttcctg cccttccagg
    35521 gtgaagatcg ggataaacga caacgtgata atcagcagac tgataaatag cgccggcccc
    35581 acttcaacag acgcatcggt gatcacctgc cagcgcgttt tattatccag cgtggcgtca
    35641 ggatgctggt gctgccactc ttccagccgt ttatgtgcat tttcgatcat gacgatagcg
    35701 gcatcgacca tcgccccgac ggcaatcgca atgccaccca acgacataat attggcattc
    35761 agtccctgga agtgcatgac aataaaagca atacacaacc ccagcggcaa cgaaataatc
    35821 gccaccagcg ccgagcgcac atgccagaga aacagcgcac agactaccgc caccacaata
    35881 aactcttcca gcaacttgcc gctgaggttg tcgatggcgc ggtcaatgag ctggctgcga
    35941 tcgtatgttg taactatctc cacgccttcc ggcagactac ttttcagcgt ttccagtttg
    36001 tccttcacgg cggcgatcac ttctcgggcg tttttgccgg atcgcaggat caccaccccg
    36061 cccgccactt caccttcgcc attcagttcg gcaatgcccc ggcgcatctc cgggccaatc
    36121 tggaccttcg caacatcacg cagataaacg ggcacgccat tttcactggc ttttaaaacg
    36181 atgtgattaa agtcgtcgag cgtttgcaga tagccgctgg cgcgcaccat atattccgct
    36241 tccgccagtt cgatcgacga accgcccgct tcctggtttg aagcatccag cgcgcttttt
    36301 acttcggcga gactgatgcc atactgcgcc aggcgctggg gatcgataac cacctgatac
    36361 tctttcacca caccgcccac cgacgccact tccgcaacgt cagggatggt tttcagctca
    36421 tatttgagaa accagtcctg taatgagcgt aaatcggcca gatcgtgctt accgctgcga
    36481 tccaccagtg catattcata gatccagcca acacccgtgg catctggccc cagctcggcg
    36541 ctgactcccg caggcagctt accctgtacc tggttgaggt actccagcac ccgcgaacgc
    36601 gcccagtacg gatcggtgcc atcttcgaaa atgacataca cataagagtc gccaaactgt
    36661 gagaaaccgc gcacagtctt cgcgccaggc accgacaaca tggtggtggt tagcggataa
    36721 gtcacctgat tttcaacgat ttgtggtgcc tgaccgggat agctggtttt aataataacc
    36781 tgcacatcgg agagatccgg cagcgcatcc actggcgtat taatgatggt ccaggtgccc
    36841 cagatgctca gaaacaacgc gcccatcagc accaaaaaac ggttcgccac cgagcgacga
    36901 ataatccatt caatcattgg ttattccctc aatgcgcatt ggtagcactt tcagagcgca
    36961 tccgctccag tgcgccagaa atattggctt cggaatcaat caggaacagg ccgctggaaa
    37021 ccaccttttc accttccgcc agaccagagc gtaatgcggt gacgccttgc gatgcctgga
    37081 aaacagcaac gcgtttcggt acaaagcgcc cgtcggcatc aacggtaatc acccgctgtt
    37141 cgctgccggt atcaatcagc gcttgtgacg gaatgagcag catcggttcg ctggcggtgt
    37201 tgagttgcaa ccaggcgttc atgcccggtt ttagcgcctc gtcggcgttg tcgacttcca
    37261 gacgcagctg cagcgtgcgg gtcgcggcat ccacaccagg tagcagcgtc catttgcgga
    37321 tggtgagtgt tttatccggt cgcgccggaa cggtgagggt aaactgcgag gcatctttca
    37381 ccagccaggc gatggactcc gggatcgcag cagtgaccca caccgggtcc ataccctgaa
    37441 ttttcgcgac cacgttatct ttggcgatat tcattcccgc gcgcagatca aacgcggtga
    37501 tcacgccatc aatgggcgct ttgagcgtaa agcgagtctg gattttttgc gtggcgatca
    37561 ggcggcgaat atccgcctcc ggcattcccg ccagtcgcag ccgctcaaga atgccttcag
    37621 tctgggtcgc cgtaccgccg gtttcgcgca gcagtaaata ctcagtctgc gcttccaccc
    37681 agtcaggaat ggtcaggtcg agaagcggtg tgcccttttg tactttatcg cccacggtaa
    37741 gcggatacac cttgtcgata aacccggcag cgcgagcctg cacaatggca tactgatact
    37801 cgttgtaact gacattcgcc gggaaactct gggcaaaagt cagcggtccg cgcgtgacgg
    37861 tagccgtttt cacccccaga ttctgcgtct gagtcgggtc aatgcgcaca ccagacgcag
    37921 aactctcttc atcggcatat ttcggcacca gatccatatc cataaacggc gatttacctg
    37981 gtttatcgaa ccgcgtattg ggatacatcg ggtcgtacca gaataagatt ttacgttctg
    38041 cggtcgacgt tttttctgca ggcggttccg cctttgcaac ccaggtaaaa cctgccgcag
    38101 aaataatacc gcccgcgatc atgctgccga taataagcgc gatttttttc atctcattaa
    38161 acctgggtta ctggctgact ttaatatcct gtaataaaga aaggttgccc tgctggacaa
    38221 aattaaacgc cactttgtcg ccggttttaa tttcactcat tttcgtctgc ggggtgatgg
    38281 taaagcgcat ggtcatctcc ggccagttca cagcagcaat cggatcgtga tggatggtga
    38341 tttttttgct ttccagatca atacccttta cctcgccagt ggcactaata acctgtggtt
    38401 gtgcttcgct catggtttca tgatgatgtt cgttagcctg ggcattaaag ccaataacgg
    38461 taaacagact gaacattgcg acttgcagtg cttttttcat ttattctctc ctggagttaa
    38521 aagttattgc tgccaaccgc caccaagtgc ggtatataaa gaaatttcgt taacctgacg
    38581 ggcataattc aggtcaagta aagtttgtcg ggttgcaaat aacgaacgtt cggcatccag
    38641 cacctccaga taacttactg cgccgtgctg atataatgcc cgcgcccgtt gcaaagtaat
    38701 ttgcagcgat gccagataac gctgctgggc gctaatttga tcgttcaggc tttgacgtaa
    38761 tgccagcgca tctgccactt ctttaaaggc gttctggatt ttctgttcat aattcactac
    38821 cgactgctgc tggcgaattt cggcgatatc cagattggcc tggttgcgtc cggcattaaa
    38881 aatggggatc tcaattttag gaataaaatt ccacatcccg ctgctggcgt taaataatga
    38941 cgacagatcg ctgctggcgg ttgatattcc gctggtcagg cttatagatg ggaaaaatgc
    39001 cgcgcgtgcg gccccaatat tggcattagc cgccattaac gcgtgttcag cttccataat
    39061 atccgggcgc tgcaataaga tttgcgacga caagcccgcc ggtaatttaa cgctttgcag
    39121 gctgtcgctg tttactgtct gcgcttgcgg cagcttgccg tagcttccca gcaacagttg
    39181 caatgcatta ttcgcctgcg ccagttcccc ctgacgttta gcgatgtcgc tgcgggtact
    39241 ttctatcacc ccgcgagcct gttccagcgc cagaacatta ctgctacctg tcaacagttg
    39301 tttttcgaca aacgcatatg actgctgata attacgcagc gtttcttcag ctatttgcaa
    39361 ttgcgcatac gccagttgct gattgaaata gctttgcgcg acattagaaa ccagcagaat
    39421 atgcaccgcg cgctgagctt cctcagtggc taaataattt tggcgctcgg cttcgctcat
    39481 gttctttaag cgaccgaaaa aatcgaggtc aaagctggcg ttaaggccgg tcgaaaactc
    39541 ccgtgtcgtg gcggtattcc ctttaagatt accgctccag ctgccgctgc cctcgccatt
    39601 gagctgtggg tagcggtcgg catcggtcag acgatattgc gcccgtgcct cctgcacttt
    39661 cagcgtcgcc atgcgcaaat cccggttatt caccagcgcc tcgctaatca gcgtcttcac
    39721 ctgattatca acaaaaaagg tgcgccagcc cgcgttctga tagttatctg ctgcgttaac
    39781 gaggccgttc tggctgagtg agaactgctg cggtacgggc attgccgggc gctgataatc
    39841 cggtgccagt gaacaaccgg ttagcgcaag ggccacacaa aatggcagaa gtttacaagg
    39901 agacataggc tcataatttc tgatgatttt atgccgccaa ctttactcgc caggctctga
    39961 ttttccggtg acaggaaaat gacaaaattg tcattttgcc aataagcgat tgccatctga
    40021 tcccgctact ctagaattgc ccgggcaaca tgcggaggaa atatgaaact gttgattgtc
    40081 gaagatgaaa agaaaaccgg agaatacttg accaaagggt taaccgaagc cggttttgtg
    40141 gtcgatttgg ccgacaacgg gctgaatggc taccatctgg cgatgaccgg tgattatgat
    40201 ctgataatcc tcgatattat gctgccggac gtgaacggct gggatatcgt gcgcatgtta
    40261 cgctccgcca ataaagggat gccgattctg ttgcttaccg cgcttggcac cattgaacat
    40321 cgcgtcaagg ggctggagtt gggggcagat gactacctga tgaaaccatt cgcttttgct
    40381 gaactgctgg cgcgggtgcg cactctcctg cggcgcgggg cggcggtaat tatcgaaagt
    40441 cagtttcagg ttgccgacct gatggtcgat ctcgtcagcc gcaaagtcac ccgcagcggc
    40501 acgcgcatca ctttgaccag taaagagttt actctgctgg agttttttct ccgccatcag
    40561 ggcgaagtgc tgccccgctc gcttatcgcc tcgcaggtgt gggacatgaa tttcgacagc
    40621 gacactaacg ccattgatgt ggcggtgaag cggctacgcg gcaaaatcga caacgacttt
    40681 gagccgaagc tgattcagac cgtgcgcggc gtgggctata tgcttgaggt gccggatggt
    40741 cagtaagcca tttcagcgcc cgttttcgct ggcaacccgt ctgacctttt ttatcagcct
    40801 ggccaccatc gcggcgtttt tcgcctttgc atggatcatg atccactcag tgaaagtgca
    40861 ttttgccgag caggatatta atgatttaaa agaaattagc gccacccttg aacgggtact
    40921 taatcatcct gacgagacgc aagcccgacg cttaatgacg ctggaagata tcgtcagtgg
    40981 ttattccaac gtgttgattt ccctggcaga tagccacggc aaaacggtgt atcactcccc
    41041 cggtgcgccg gatatccgcg agtttacgcg tgacgccata cccgataaag acgcgcgggg
    41101 cggtgaggtg tatctccttt ccggcccgac gatgatgatg ccaggccacg gtcacgggca
    41161 tatggaacac agcaactggc ggatgattaa cttgccggtt ggcccgttgg tggacggcaa
    41221 accgatttat acgctctaca tcgcgctttc gatcgatttt catcttcatt acataaatga
    41281 tttgatgaat aaacttatta tgaccgcatc tgtcatcagc atcctgatcg tctttatcgt
    41341 gctattggcg gtacataaag gtcacgcgcc gatccgcagc gtcagccgtc aaatccagaa
    41401 tatcacctcg aaagatctcg acgttcgcct tgacccgcag accgtgccca ttgagctgga
    41461 acagctggta ctgtcgttca accatatgat cgagcgtatt gaggatgtct ttacccgcca
    41521 gtccaatttc tcagcggata tcgctcacga aattcgcacg ccgattacca atctcatcac
    41581 gcaaacggaa atcgccctca gccagtctcg cagccagaag gagctggaag atgtgctcta
    41641 ctctaatctc gaagagctga cgcgaatggc gaaaatggtc agcgatatgc tgtttctcgc
    41701 tcaggccgat aacaaccagc taatccccga aaagaaaatg ctcaacctgg cggatgaagt
    41761 cggcaaagtg ttcgattttt tcgaggcgtt agcggaagat cgcggcgtgg agttgcggtt
    41821 tgttggcgac aaatgtcagg ttgcgggcga tccgctgatg ctgcgtcggg cgttaagcaa
    41881 cctgctttct aatgccctgc gttatacgcc acccagtgag gctattgtag tgcgctgcca
    41941 gacggtcaat catcaggtgc aagtttccgt cgaaaacccc ggtacgccca ttgcgcccga
    42001 gcacttaccg cgattgtttg accgtttcta tcgcgttgac ccttcccgcc agcgaaaagg
    42061 tgaaggtagc ggtattgggc tggcgatagt gaaatcgatt gttgtcgcgc ataaaggcac
    42121 ggttgcggta acgtcagatg cgcgggggac aaggtttgtg attatgctgc cggagagaga
    42181 gtgattatta agttcaaatt cagggcgtgg tgcatcacct cctggtattg ttgatcaac
//
LOCUS       DAZFRN010000023        41953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFRN010000023 DAZFRN010000000
VERSION     DAZFRN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.023"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            20..1303
                     /gene="yebS"
                     /locus_tag="ACHKUB_001215"
     CDS             20..1303
                     /gene="yebS"
                     /locus_tag="ACHKUB_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO6654257.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            1272..3905
                     /gene="yebT"
                     /locus_tag="ACHKUB_001216"
     CDS             1272..3905
                     /gene="yebT"
                     /locus_tag="ACHKUB_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO6654258.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            3985..5424
                     /gene="rsmF"
                     /locus_tag="ACHKUB_001217"
     CDS             3985..5424
                     /gene="rsmF"
                     /locus_tag="ACHKUB_001217"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO6654259.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            5542..5778
                     /gene="yebV"
                     /locus_tag="ACHKUB_001218"
     CDS             5542..5778
                     /gene="yebV"
                     /locus_tag="ACHKUB_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO6654260.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            5883..6074
                     /gene="yebW"
                     /locus_tag="ACHKUB_001219"
     CDS             5883..6074
                     /gene="yebW"
                     /locus_tag="ACHKUB_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO6654261.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(6075..6731)
                     /gene="pphA"
                     /locus_tag="ACHKUB_001220"
     CDS             complement(6075..6731)
                     /gene="pphA"
                     /locus_tag="ACHKUB_001220"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6654262.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(7127..7468)
                     /gene="yebY"
                     /locus_tag="ACHKUB_001221"
     CDS             complement(7127..7468)
                     /gene="yebY"
                     /locus_tag="ACHKUB_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO6654263.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(7481..8353)
                     /gene="copD"
                     /locus_tag="ACHKUB_001222"
     CDS             complement(7481..8353)
                     /gene="copD"
                     /locus_tag="ACHKUB_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO6654264.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(8357..8731)
                     /gene="yobA"
                     /locus_tag="ACHKUB_001223"
     CDS             complement(8357..8731)
                     /gene="yobA"
                     /locus_tag="ACHKUB_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO6654265.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            8870..9100
                     /gene="holE"
                     /locus_tag="ACHKUB_001224"
     CDS             8870..9100
                     /gene="holE"
                     /locus_tag="ACHKUB_001224"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO6654266.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9202..9858
                     /gene="yobB"
                     /locus_tag="ACHKUB_001225"
     CDS             9202..9858
                     /gene="yobB"
                     /locus_tag="ACHKUB_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO6654267.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            9882..10544
                     /gene="exoX"
                     /locus_tag="ACHKUB_001226"
     CDS             9882..10544
                     /gene="exoX"
                     /locus_tag="ACHKUB_001226"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO6654268.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(10541..12601)
                     /gene="ptrB"
                     /locus_tag="ACHKUB_001227"
     CDS             complement(10541..12601)
                     /gene="ptrB"
                     /locus_tag="ACHKUB_001227"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO6654269.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(12810..13469)
                     /gene="yebE"
                     /locus_tag="ACHKUB_001228"
     CDS             complement(12810..13469)
                     /gene="yebE"
                     /locus_tag="ACHKUB_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO6654270.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(13796..14152)
                     /gene="yebF"
                     /locus_tag="ACHKUB_001229"
     CDS             complement(13796..14152)
                     /gene="yebF"
                     /locus_tag="ACHKUB_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO6654271.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(14219..14509)
                     /gene="yebG"
                     /locus_tag="ACHKUB_001230"
     CDS             complement(14219..14509)
                     /gene="yebG"
                     /locus_tag="ACHKUB_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO6654272.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            14643..15821
                     /gene="purT"
                     /locus_tag="ACHKUB_001231"
     CDS             14643..15821
                     /gene="purT"
                     /locus_tag="ACHKUB_001231"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO6654273.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(15877..16518)
                     /gene="kdgA"
                     /locus_tag="ACHKUB_001232"
                     /gene_synonym="eda"
     CDS             complement(15877..16518)
                     /gene="kdgA"
                     /locus_tag="ACHKUB_001232"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO6654274.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(16555..18366)
                     /gene="edd"
                     /locus_tag="ACHKUB_001233"
     CDS             complement(16555..18366)
                     /gene="edd"
                     /locus_tag="ACHKUB_001233"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO6654275.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(18601..20076)
                     /gene="zwf"
                     /locus_tag="ACHKUB_001234"
     CDS             complement(18601..20076)
                     /gene="zwf"
                     /locus_tag="ACHKUB_001234"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO6654276.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            20414..21283
                     /gene="hexR"
                     /locus_tag="ACHKUB_001235"
                     /gene_synonym="yebK"
     CDS             20414..21283
                     /gene="hexR"
                     /locus_tag="ACHKUB_001235"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO6654277.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            21411..22853
                     /gene="pyk"
                     /locus_tag="ACHKUB_001236"
     CDS             21411..22853
                     /gene="pyk"
                     /locus_tag="ACHKUB_001236"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO6654278.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(22985..23956)
                     /gene="lpxM"
                     /locus_tag="ACHKUB_001237"
                     /gene_synonym="msbB"
     CDS             complement(22985..23956)
                     /gene="lpxM"
                     /locus_tag="ACHKUB_001237"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO6654279.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(24076..25398)
                     /gene="mepM"
                     /locus_tag="ACHKUB_001238"
     CDS             complement(24076..25398)
                     /gene="mepM"
                     /locus_tag="ACHKUB_001238"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO6654280.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(25414..26346)
                     /gene="znuA"
                     /locus_tag="ACHKUB_001239"
     CDS             complement(25414..26346)
                     /gene="znuA"
                     /locus_tag="ACHKUB_001239"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO6654281.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            26425..27180
                     /gene="znuC"
                     /locus_tag="ACHKUB_001240"
     CDS             26425..27180
                     /gene="znuC"
                     /locus_tag="ACHKUB_001240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO6654282.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            27177..27962
                     /gene="znuB"
                     /locus_tag="ACHKUB_001241"
     CDS             27177..27962
                     /gene="znuB"
                     /locus_tag="ACHKUB_001241"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO6654283.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(28109..29119)
                     /gene="ruvB"
                     /locus_tag="ACHKUB_001242"
     CDS             complement(28109..29119)
                     /gene="ruvB"
                     /locus_tag="ACHKUB_001242"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO6654284.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(29128..29739)
                     /gene="ruvA"
                     /locus_tag="ACHKUB_001243"
     CDS             complement(29128..29739)
                     /gene="ruvA"
                     /locus_tag="ACHKUB_001243"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO6654285.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(29878..29943)
                     /gene="yobI"
                     /locus_tag="ACHKUB_001244"
     CDS             complement(29878..29943)
                     /gene="yobI"
                     /locus_tag="ACHKUB_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO6654286.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            30014..30616
                     /gene="yebB"
                     /locus_tag="ACHKUB_001245"
     CDS             30014..30616
                     /gene="yebB"
                     /locus_tag="ACHKUB_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO6654287.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(30618..31139)
                     /gene="ruvC"
                     /locus_tag="ACHKUB_001246"
     CDS             complement(30618..31139)
                     /gene="ruvC"
                     /locus_tag="ACHKUB_001246"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO6654288.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(31174..31914)
                     /gene="yebC"
                     /locus_tag="ACHKUB_001247"
     CDS             complement(31174..31914)
                     /gene="yebC"
                     /locus_tag="ACHKUB_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6654289.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(31943..32395)
                     /gene="nudB"
                     /locus_tag="ACHKUB_001248"
     CDS             complement(31943..32395)
                     /gene="nudB"
                     /locus_tag="ACHKUB_001248"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO6654290.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(32513..34285)
                     /gene="aspS"
                     /locus_tag="ACHKUB_001249"
     CDS             complement(32513..34285)
                     /gene="aspS"
                     /locus_tag="ACHKUB_001249"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO6654291.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            34595..35161
                     /gene="yecD"
                     /locus_tag="ACHKUB_001250"
     CDS             34595..35161
                     /gene="yecD"
                     /locus_tag="ACHKUB_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6654292.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADHAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            35158..35976
                     /gene="yecE"
                     /locus_tag="ACHKUB_001251"
     CDS             35158..35976
                     /gene="yecE"
                     /locus_tag="ACHKUB_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO6654293.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            36029..36424
                     /gene="yecN"
                     /locus_tag="ACHKUB_001252"
     CDS             36029..36424
                     /gene="yecN"
                     /locus_tag="ACHKUB_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO6654294.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            36465..37208
                     /gene="cmoA"
                     /locus_tag="ACHKUB_001253"
     CDS             36465..37208
                     /gene="cmoA"
                     /locus_tag="ACHKUB_001253"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO6654295.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            37205..38176
                     /gene="cmoB"
                     /locus_tag="ACHKUB_001254"
     CDS             37205..38176
                     /gene="cmoB"
                     /locus_tag="ACHKUB_001254"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO6654296.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLIPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(38341..40770)
                     /gene="torZ"
                     /locus_tag="ACHKUB_001255"
     CDS             complement(38341..40770)
                     /gene="torZ"
                     /locus_tag="ACHKUB_001255"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO6654297.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRSSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAGLRKKYAVADREPITIHTEDATRFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(40795..41895)
                     /gene="torY"
                     /locus_tag="ACHKUB_001256"
     CDS             complement(40795..41895)
                     /gene="torY"
                     /locus_tag="ACHKUB_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO6654298.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKTTDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKVLTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         9940 a        10866 c        10441 g        10706 t
ORIGIN      
        1 ttattacatt tggcacgtca tggctcttaa cacaccacaa attacgccga caaaaaagat
       61 aacagtgagg gcaatcggcg aggaactgcc gcgtggtgat taccaacgtt gcccgcaatg
      121 tgacatgctg tttagcctgc ccgagataaa ttctcatcag agtgcctatt gtccgcgctg
      181 tcaggcaaaa attcgcgacg ggcgcgactg gtcgctaacg cgcctggcgg caatggcttt
      241 caccatgctg ttgctgatgc cgtttgcctg gggcgaaccg ctgttgcata tctggctgtt
      301 aggtattcgt atcgacgcca acgtcatgca aggcatctgg caaatgacca aacagggcga
      361 tacgataacg ggggcgatgg tctttttctg cgttattggt gcccccctca ttctggtgtc
      421 ctccatagca tatttatggt ttggtaaccg actgggaatg aatttacgtc cggtactgct
      481 gatgcttgag cgacttaaag agtgggtaat gctggatatc tacctggtcg gcattggcgt
      541 tgcttctata aaggtacagg attatgccca tatccaggcg ggtgtcggct tgttctcttt
      601 tgtggcgttg gtgattttaa cgacggtgac gttgtcacat cttaatgtcg aggagctgtg
      661 ggagcgattt tatccgcagc gccccgctac gcgtagggac gagaaacttc gtgtctgtct
      721 tgggtgccat tttaccggct atcctgatca gcgtggtcgc tgcccgcgtt gccatatccc
      781 gctacgcctg cgtcgccgtc atagtttgca aaaatgctgg gcggcgctgt tagcgtcaat
      841 cgttttgttg ttacctgcca acctgttgcc tatttctatc atttatctga acggaggacg
      901 gcaggaagat acaattcttt ccggaattat gtcgctggca agtagcaaca ttgcggttgc
      961 gggaatcgtg tttatcgcca gtattctggt accgtttact aaagtgatcg tcatgttcac
     1021 tttactgttg agcattcatt ttaaatgcca gcaaggttta cgcacacgca ttctgttact
     1081 gcggatggtg acctggattg gtcgctggtc gatgctcgac ctgtttgtca tatctttaac
     1141 catgtcgctg attaatcgcg atcagatcct cgcttttact atgggaccgg ctgcgtttta
     1201 ttttggcgca gccgtaattt tgactattct tgctgtggaa tggctggaca gccgcttact
     1261 ttgggatgca catgagtcag gaaacgcccg cttcgacgac tgaagcgcag attaaaaata
     1321 aacgccgtat ctcacctttc tggctgctgc ctttcatcgc gctaatgatt gccggttggc
     1381 tgatttggga cagttatcag gaccggggta ataccgtcac catcgacttt atgtcggcgg
     1441 atggtattgt tccgggccgt acgcctgttc gttatcaggg cgttgaagtc ggaacagtgc
     1501 aggatatcag cctcagcgac gatcttcgta agattgaagt caaggtcagc atcaagtccg
     1561 atatgaaaga tgcgctgcgc gaagagactc aattctggct ggtgacgcca aaagcatcgt
     1621 tggcaggtgt ctccgggctg gacgccctcg tcggtgggaa ctatatcggc atgatgccgg
     1681 gtaaaggtaa agagcaggat cactttgtcg cactcgatac ccaaccgaaa tatcggctgg
     1741 acaatggcga tctgatgatc cacctgcaag cccccgatct cggttcgctg aacagcggtt
     1801 cattggtcta tttccgcaag atcccggtgg gaaaagtcta cgactatgcc atcaatccca
     1861 acaaacaagg cgtggtgatt gatgtcctga tcgagcggcg ttttaccgat ctggtgaaaa
     1921 aaggtagccg tttctggaac gtttccggcg ttgatgccaa tgtcagtatc agtggcgcga
     1981 aggtgaaact ggaaagcctg gcggcactgg ttaacggtgc gattgccttc gattcaccag
     2041 aagagtcgaa acctgccgag gcggaagata cctttggtct gtatgaagac ctcgcccata
     2101 gccagcgtgg cgtaataata aaactggaac tgccgagtgg ggccggatta accgccgact
     2161 cgacgccatt aatgtatcag ggactggaag tcggacagct aactaaactg gatttaaatc
     2221 ctggtggtaa agtcactggg gaaatgacag ttgatcccag cgtcgttacc ctgcttcgtg
     2281 aaaatacccg catcgaatta cgtaatccga aattatccct tagcgatgcc aatctcagcg
     2341 ccctgctgac tggcaaaacc ttcgagttgg tacccggcga tggcgagcca cgcaaagagt
     2401 tcgttgttgt gccaggcgaa aaagcactgc tgcatgaacc tgatgttctg acgctgaccc
     2461 tgaccgcgcc ggaaagttac ggtattgatg ctggtcagcc gctcattctt cacggcgtgc
     2521 aggtaggcca ggttatcgat cgtaaactca ccagcaaagg cgtcaccttt accgtcgcca
     2581 tcgagcctca gcatcgagaa ctggtaaaag gcgatagcaa atttgtcgtc aacagccgtg
     2641 tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg tgccagcgcc tcagaatgga
     2701 ttaacggcgg gatacgtatt ctgccgggcg ataaaggcga gatgaaagcc agctatccac
     2761 tgtatgccaa tctggaaaaa gcgctggaga acagccttag cgatttaccc accacaaccg
     2821 tgagtttgag tgcagagacg ctgccggatg tgcaggcagg atcggtagtg ctctaccgta
     2881 aatttgaagt tggtgaagtt attaccgtgc gtccgcgagc taacgcgttt gatatcgatc
     2941 tgcatattaa gccggagtat cgcaaccttc tgaccagcaa tagcgtgttc tgggcagaag
     3001 gcggggcgaa agttcagctg aatggtagtg gcctgaccgt acaggcatcc ccgctctcca
     3061 gagcattaaa aggggctatt agcttcgata acctcagtgg tgccagcgcc agtcagcgta
     3121 aaggcgacaa acgtattctg tatgcttccg aaacagcggc ccgtgcggtt ggtgggcaga
     3181 ttacgcttca cgctttcgat gccggaaaac tggcggtcgg gatgccaatt cgctatctcg
     3241 gtattgatat cgggcaaatc cagacgctgg atctgattac cgcgcgcaat gaagtacagg
     3301 caaaggcggt gctctatccg gaatatgtcc agacctttgc tcgcggtggt acgcgcttct
     3361 cagtggtcac accgcaaatt tcggcagctg gcgttgagca tctagatact atcctccagc
     3421 cgtatatcaa cgtcgaacca ggccggggca atcctcgccg cgactttgaa ttacaagagg
     3481 ccaccattac tgattcgcgt tacctggatg gcttaagcat tattgttgaa gcgccggaag
     3541 ccggttcgtt aggtattggt acgcctgtgc tgttccgtgg tctggaagtc ggtacggtta
     3601 caggaatgac gctggggaca ttgtcagatc gcgtgatgat tgcgatgcgc atcagtaaac
     3661 gctatcaaca cctggtgcgt aacaattccg tcttctggtt ggcatcgggt tacagtctgg
     3721 actttggtct gacgggcggc gtagtgaaaa ccggcacctt taaccagttt atccgtggcg
     3781 gcatcgcctt cgccacgcct ccgggtacgc cactggcacc gaaagcccag gaaggcaaac
     3841 acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga atggggaact gcgcttccca
     3901 aataatgccc actgctccgg cgtgcctgcg ccggagcgtt tatgctaaac tgcgcgcctg
     3961 tttttttgcc agtggtacat gctcgtggcc caacacaccg tttatttccc ggacgccttt
     4021 ctgacacaaa tgcgcgaagc gatgccttcg acgctctcat ttgatgattt tcttgccgcc
     4081 tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc tgaaaatctc cgttgctgat
     4141 ttcctgcaat taaccgctcc ttatggctgg acgcttacgc caattccgtg gtgtgaagaa
     4201 ggtttctgga ttgaacgcga caatgaagat gcattgccat tgggtagtac cgccgagcat
     4261 ttaagcggcc tgttttatat tcaggaagcc agttcaatgt tgcccgtcgc cgccttgttt
     4321 gctgacggta atgcaccaca gcgggtgatg gatgtcgctg ctgcgccggg ctccaaaacg
     4381 acgcaaattg ccgcgcggat gaataacgaa ggagcgatcc ttgccaatga gttttccgcc
     4441 agtcgggtaa aagtgttaca tgccaatatc agccgctgtg gcatcagtaa tgttgcgctc
     4501 acacattttg atggccgcgt gtttggtgcg gcagtgccag aaatgttcga tgccattttg
     4561 ctggacgctc cctgctctgg cgaaggcgtg gtgcgtaaag atcccgatgc gctaaaaaac
     4621 tggtcaccag aaagcaatca ggaaatcgca gctacacaac gggagcttat cgacagcgcc
     4681 tttcatgcat tacgtcctgg tggtacgctg gtttactcga cctgtacctt aaaccaggaa
     4741 gaaaacgaag ccgtttgcct gtggctgaaa gagacttacc ccgacgcagt agagttttta
     4801 ccacttggcg atctcttccc tggtgcaaac aaagcgctga ccgaggaagg ctttttgcat
     4861 gttttcccac aaatttacga ctgcgaaggc ttcttcgttg ctcgtctgcg taaaactcag
     4921 gcgatccccg ccttacccgc ccccaaatac aaagtgggca atttcccgtt cagcccggtg
     4981 aaagatcgcg aagccggaca aattcgtcag gcggctgcaa gtgttggctt aaactgggat
     5041 ggaaacctgc gactctggca acgcgacaaa gaactgtggt tgttcccggt gggcattgaa
     5101 gccctgatcg gtaaagtccg attttctcgc ttggggatta aacttgccga aacgcacaac
     5161 aaaggttatc gctggcagca tgaagcagtt attgcccttg ccacccccga caatgtgaac
     5221 gcttttgaac tgacaccgca ggaagcggag gagtggtatc gcgggcgcga tgtttacccg
     5281 caagccgcgc cagtggcgga tgacgtgttg gttactttcc agcatcaacc gattggttta
     5341 gccaaacgga ttggttcgcg attgaaaaac agctatccgc gtgaactggt gcgcgatggg
     5401 aaacttttta ccggtaacgc ctgacagcgc acaaaaaaag cgcacttttt gagtggcaca
     5461 ttcggctgcc tcaactaggc tgaaaaatgg tgcgatcgga ctggtcgtac cacaaccggc
     5521 agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca taggcgcttt tgaaatcgac
     5581 gacggcgaat tacacggtga atcgccaggt gatcgaacgt taaccattcc ttgtaaatct
     5641 gaccccgatt tatgtatgca actggatgcc tgggatgctg aaaccagtat ccctgccctg
     5701 ctaaatggcg aacactctgt cctttaccgt acccgttacg atcaacaatc tgatgcctgg
     5761 attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat ggcgggttat ttgtcctggt
     5821 tattcccccg ttgtaaaatc tctcctaaac ttaacggtac ggcaccacac ttcggggatg
     5881 aaatgttcgc gctggtactt tttgtttgct acctggatgg cggttgtgaa gatattgttg
     5941 tggatgtcta caacacggag cagcagtgtc tttattctat gagcgatcaa cggatccgcc
     6001 atggcggttg ttttccgatt gaggatttta tagatggttt ctggcgacca gcacaggagt
     6061 atggtgattt ttaattattg caattgcaca agagtcagtt cgcccccaaa gacagcaccg
     6121 gtatcaatat aatgcaggtt gccaatatcc acgcgatgtc gcaacggtgt atgaccaaac
     6181 cagaaatgat cagcacctgt aattccctgc cctttttggc gttcacctaa tcgcgagcgg
     6241 ctccacaaga cctgatgcaa atcaacgtcc ttttgccatt cataaacatc atctggataa
     6301 tcggcatgag caataacatg tttgccggta cgactgtgta cttcaagaat aaagggcaaa
     6361 tgctgacatt tttccagcgc cgttttcgct tgtttctgtt gattatctgc cagcgcaata
     6421 aaccagtcgc cgccattcat caaccacaaa gacatctgct gggatgccag cgcatccatc
     6481 gccatctgtt catgattgcc tcttaccgca caaacccaat gttgttccag taactgcaga
     6541 caacgtaaac tttgcggccc acgatcgata acgtctccta ctgagataag taaatctcgc
     6601 cacggatcaa aacgacaatg ccataatttg cggcgcaact gctcaagaca accgtgtata
     6661 tcgccagaaa gccagatatg tcgccattga tgacccgcaa ttctctgata aacgggcgca
     6721 ggctgtttca tcaatatttt cctcccgcgc taaagatcac ataatcttaa caagaatgtt
     6781 aaaaaacgct ggactcagac agtagagtgt gtgttatggt tgactataaa gtcagcgaag
     6841 gaaatgcttc tggcttttaa cagataaaaa gagaccgaac acgattcctg tattcggtcc
     6901 agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt
     6961 tgccttgccc tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt
     7021 caataaaaag cgtggtggtc atcagctgaa atgttaaaaa ccgcccgttc tggtgaaaga
     7081 actgaggcgg tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt
     7141 cagctcgttc tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa
     7201 tctgaatcac cgaaatgggt tgggctttag tcttcccact ggcaacttcc ttttgtgcga
     7261 tatcgtttaa aggatactgc acgagggtac ttgggttaat gacatacaaa gcattacccg
     7321 gtctgcaagt cagcatcacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa
     7381 aacgactgac ggtaatcacc tgcggtgcag ccagcgccgc tgcagaactg gtgagtaaca
     7441 gaaacgtcag aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg
     7501 caagcaggct gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc
     7561 actcaaacca gggtgccgca cgatcttcat cttgcctcat gcgcggtaca agaacataac
     7621 gattagccag cgcaatgacc accataatca tcaccaggat ggctttgagc aataagagtt
     7681 ggccccagta agtcgtaagc gtggggggaa atccggtaat caacaatgca ttaagcacgc
     7741 cgcttgccag tacgccgatc accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct
     7801 gaatggcctg gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta
     7861 atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt
     7921 tcgccgttac cccttcattc agtgtcgcat gcccgattcc cgccagcaga ataaattgcg
     7981 cggtagtgag cataaacagc aaacgtggca tattccgcgg ttgcataagg gcgacgatca
     8041 acgttacgag ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa
     8101 cagcctgcca aatgtttggc gaaaatacat ctgcccatcc cgttcccatc aacccgccct
     8161 gaacggcgag cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac
     8221 gcagaaaacg cttcgccagc aagcgacgaa tcgttaaggg ggccagccag gcaccgtaca
     8281 ttgcgaagcc aaagaccagc atcagcgagg tgaaatggat aaatcgcagc gcgatccagg
     8341 taaacgccag catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt
     8401 ccaccgaaac aacatgccag tcaacgatgt aggttccggg tttcagcgaa tcggccagtg
     8461 gaacaatcag ttgcttttgg tcctgctcat ttcgcttcgc cggtaatgtt ttaatatttt
     8521 cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt ttcaacacct tccgagaagt
     8581 ttaaagtgat tgcctgcggt gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg
     8641 ttaaatgcgc atgtgcccag acggaaggtg tcaccaacga agtggtcagg attgccagcg
     8701 cgtagcgaag ggagcgtgca gttgaagtca tattatctat tcctttttgt aataactttt
     8761 ttacagagca taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc
     8821 ctggctcact cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa
     8881 tctggctaaa ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg
     8941 ggtggcattt aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca
     9001 gcctgaacat ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt
     9061 caatctgtca cgtttacctt acgagcccaa acttaaataa aacttataca gagttacact
     9121 ttcttacata acgcctgcta aattatgagt attttctaaa ccgcacccat aatttgcagt
     9181 cattttgaaa aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct
     9241 cgtaaaacct cactcaccga acaggtagct catcatctgg agtttgtgag ggccgccgcc
     9301 aggcaacaat gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg
     9361 cgacgtgccc ttcctgcccc acccgatctc tcactgttag acccgctttg ttatgccgca
     9421 acgacctggc gaatgaccat cattgccggc cttcccgttg aatataacga tcgctttatt
     9481 cgtggaattg cggtgttcgc accgtggcga aaaacgcctg ggatctacca tcagagtcat
     9541 ggtgcatgtc tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca accgcaaggt
     9601 atggatatgg acccgacctg ttcactgttt actacagggc aatgtctggg agaacccgac
     9661 ctgctggcgt cggcccgtcg cttacaattt ttttcacatc agtactcgat tgccgtgctg
     9721 atggcaaatg cccgtggtaa cagtgcacta tgggatgaat atggtcgtct catcgttcgc
     9781 gccgatcgtg gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat
     9841 atcattccat tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata
     9901 cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg
     9961 gaaaaatcgt caaccccatg agccacctgg tgcgccccga tcgtcctatt agtccacaag
    10021 cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg
    10081 tgatcccaca ctattacggt agtgaatggt atgtcgcgca taacgccagc tttgaccgcc
    10141 gcgtactgcc tgagatgccc ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt
    10201 ggcctgggat caagtacagc aatatggcgt tatataaaac acgcaagctc aatgtacaga
    10261 cgccgccggg cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc
    10321 ttatcgatat tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac
    10381 gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg
    10441 ttgccgaacg cgatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc
    10501 cggagctgcg tttaacactg aaacattatc tggaaaatac ttagtccgca ggcgtagcgg
    10561 gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg
    10621 atttaaagcg accagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa
    10681 gatggtcatc ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatatt
    10741 gcacctgaga atcgtgtaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga
    10801 cgttgtcata cgggctgtag cttttcatgt actcgtaata ttgcggatcc tgcgggttac
    10861 cccactcttc aaactcacca gtggtaagag gaattgattc atcaagcatc gttgttacaa
    10921 catcaacaaa cggtacctgg gcgataacgc cgtggaataa ttccgggcgt tgattaattg
    10981 caacgcccat caacatgccc cccgcactcc cgcccatcgc ataacaaagc gaaggagagc
    11041 catagcccag ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct
    11101 tcttcagaaa tttaccgtct tcgtaccatt gttgccccag ctcaccgccg ccgcgaacat
    11161 ggacaatggc gtagacaaat ccacgatcta ataaactcaa gcggctaaaa ctgaaatcgg
    11221 catcaatact tgcgccgtaa gaaccgtagc catacaccag cagcgggttg tgccctttgc
    11281 gaaaatgttt gcgatggtag accaacgaaa ccggaacttc gacgccatca cgggcgacta
    11341 tccacaggtg ttcactgcgg taattcgccg catcaaaacc aggaacttcc gtttgtttta
    11401 atacacgacg ctcaccggta tccatatcca gttcaaacaa agtgtctggt gtggtcatgg
    11461 aagaataacc ataacgcaat cgcgcggttt caggttctgg attgtaggca atccaggtca
    11521 cataggccgg atcatcaaag gcaatgccaa tgacttcccg ggtcttgcgg ttaatttggc
    11581 gcaaactggt taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa
    11641 acccttccag catgatgttt tcgcgtggcg gaattaactc ttcccactgt tgctcatcac
    11701 gcatacgggt acggtataag ccaaagtttt tgccgtggcg attggaacgc agataaaaac
    11761 gatgctggta gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg
    11821 gctcggcatc ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca
    11881 aatgaatgac tacatagtgc ttcgaggtcg ttttatgcag gctgacgtaa taggtatcgt
    11941 ctttttcttc gtagatcagt ttatcttgcg atgctggcgt accgatggcg tgacgccaga
    12001 cctgataagg cagcagcgtc accggatgct tgcgaacata gtagaaagtc caggagtcat
    12061 ttgcccagac aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttaccagttt
    12121 ccagattacg aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca
    12181 taatggtgtt atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagctg
    12241 cgcgcttatt ggcatcgagc aatgtttccc actcatccca ctcttcactg aatgccgatt
    12301 gacgctggta gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat
    12361 ttttgatgta gggcgcagaa acttctcgtt gcggaatgcg gtcgatgatt tcctttaaga
    12421 tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt
    12481 gcaggtagtc caggacttct ggctgagaac gcgtatcgtc ccgcagccag tagtaattat
    12541 cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca
    12601 tgttattgtt ctttctggtt gaaacatctt ataagggtgg caaaactcac cggggatgca
    12661 agcgaaacag gggagtcatt gcttagatga tgacaggtaa tggcgcggat atcgaatgtt
    12721 atgcagacag agaaaatcag cctgttcaaa tggctgtgcg attctggata gcccgaaata
    12781 gtcaacttca ggctatccag agagcggaat tattccgcca aagtgcgttt ttgctgttcg
    12841 agatcgcgtt caatgccgtc acgaacatcc tgggggattt tcagcgcgtc acccagtgca
    12901 ttcaggtaac tgcgttccat aaaatggtca atatcaatag ccgcgcaact caggaaatag
    12961 atttccagcg cctcttcttc attgcggacc ccggtagcca ggcgttgtgg atccagcggt
    13021 tgttcaattg cctgctcaat gagtacacgc ccctgctctt ccacgccggc ttcacgcaat
    13081 tgctggtcga ttgccgcacg ttctttggca tcaatatgac catcactttt agcggcaaag
    13141 accagcgcaa ggatcaaacg ttctgtacgc tcatccagcg gcgtactttg cgcgccaaac
    13201 tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt atttattcca cagcaccgta
    13261 cccgctaccg ctccgccgcc aaccagtaac gcgttggtgc catatttcgt aagaagttta
    13321 cgtgctgatt tatttgcgac cagcagccca gccagtccgc ctaatgcgcc tggcactaac
    13381 agtttgccca atccctgatc cgcagacgag gaggtagaag aactgctttg cccaagcagg
    13441 gattgcagtt gatttaacca gttagccata tttgctcctc aataaccatt tttagtgcct
    13501 gtagcatagc ggatgaggat gtcagaaaat gtatatcgag acaaaagatg cgcaaataag
    13561 cggggcggta ggccgttatt caaagaaatt cgcgccaggt aaggtattta ctgtatatca
    13621 ggtgataagg gtattttagg tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct
    13681 gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac gattgtggtg cgttctgggg
    13741 cggacaaggc actcgcgccg catccgccaa cggtcaatcg aattatcaga agccattaac
    13801 gccgctgata ttccgccatt cccgctttgc agtccacgct gacctggtaa tgaatatcgg
    13861 cacttttacc acgcacggtt agcggtactg accatttatc atctttaccc tgaatgtcct
    13921 gcaaactgac ccaagccacg ggatcggcct gaccgacaat tttttgatca tctgcccagc
    13981 gcgccacgcg attttgttga taatcacgtt ttacgctcgc ggcaattccg gcagcatcca
    14041 gaccttcaca ctttgggaaa gtgaccgact tgctggtttc attattggca gcgaaaactg
    14101 atgcgcaggc agaaaccaac aacagcccta aaaacgcccc tctttttttc atgtttttct
    14161 ccatagcaca atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc
    14221 aggccgcgtg tgaggcatct tcctcttcgg attcagcacc gacgacctgt ggggacggta
    14281 atttgccggt tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa
    14341 gggcttcacg ttgctcgtct tccatttgca ctggagaatt tgtcagccag gtgtcaagca
    14401 gatccgccgt atcaagcatt ttatcataag catcggcttc ctttttgctg gtaaacgaca
    14461 ttttctcttc gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct
    14521 cataataact gtgattttat acagtatatt tcttttcggt tgagaaatca acatcagcaa
    14581 taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg
    14641 ttatgacgtt attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct
    14701 ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg
    14761 ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta
    14821 atatgcttga tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg
    14881 tgccggagat cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga
    14941 atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc
    15001 tggcggcaga acagctgcag ctgcccactt ccacttatcg ttttgccgat agcgaaagcc
    15061 ttttccgcga ggcggttgcc gccattggct atccctgcat tgtaaaaccg gtgatgagct
    15121 cttccggcaa ggggcagacg tttattcgct ctgcagagca acttgctcag gcatgggaat
    15181 acgctcagca aggcggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt
    15241 ttgacttcga aattaccctg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac
    15301 cagtaggtca tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga
    15361 gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg
    15421 gttatggtct gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg
    15481 tctcccctcg tccacatgat accgggatgg tgacgttaat ttctcaagat ctctcagagt
    15541 ttgccctgca tgtacgtgcc ttcctcggac ttcctgttgg cgggatccgt cagtatggtc
    15601 ctgcagcttc tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg
    15661 tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg
    15721 gcagccgtcg tctgggtgtt gcgctggcga ccgctgagag tgttgttgac gccattgaac
    15781 gcgcgaagca cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaaacgcta
    15841 caaaaatgcc cgatcgtaga tcgggcgttt tgacttttac agcttagcgc cttctacagc
    15901 ttcacgcgcc agcttagtaa tgcggtcgta atcgcccgct tccagcgcat ctgccggaac
    15961 cagccaggaa ccaccgatgc acagcacgct tttcagcgcc aggtagtcac ggtagttagc
    16021 cggagaaata ccacccgtcg ggcagaaacg gacctgggag aacggacccg cgatcgcctg
    16081 cagggctttc acgccgccgt tagcttcagc cgggaagaat ttgaactctt tcaaaccgta
    16141 gtccataccc agcatcagtt cggaaacagt gctgatcccc ggaattagag gaatagtccc
    16201 ttcggtagca gctttcagca gcggctcggt cagacccggg ctaattgcga actgtgcacc
    16261 cgcttcagtg acttctgcca gctgctgtgg attcagcacc gtaccggcac ccacaatcgc
    16321 ttcaggcact tctttggcga tagcacggat agcgtcaact gcacactcgg tacgcagagt
    16381 cacttccaga acgcgcaccc caccagcaac caacgctttt gccatcggca ctgcgtgttc
    16441 cagttttttt accacgataa ccggtacaac cgggccggtg gtcaggattg attctgcact
    16501 tgttttccag tttttcatca gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa
    16561 agtgatacag gttgcgccct gttcggcacc ggacagtttt tcacgcaagg cgctgaataa
    16621 ttcacgtcct gtccccacgc gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc
    16681 cagttccgct tcgtctacca gcagcgtcag ttcgcctgtc tgtccattca cacgaatgat
    16741 gtccccgtcg cgcacttttg ccagcagccc gccatcgtag gcttctggtg ttacgtggat
    16801 agctgacggc actttacctg aagcgccgga gagtcgtcca tcggtaacta acgcaatttt
    16861 gaaacaccgg tccaataata caccaagtgg cggcatgagt ttatgtaatt ctggcattcc
    16921 gttcgctttt ggcccctgat gacggacaac aacgacacaa tcgcggtcca gcaaacccgc
    16981 ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca accgctggcg cttcaatcac
    17041 ctggttctca accggcacgg cagaggtttt cataaccgca cggcccaggt taccgcttaa
    17101 cacttttgtc ccaccatgat gagagaaagg ttgttcgaag gaagcgatca cattgctgtc
    17161 gagtgatttt tccgcccctt cccgccagtc cagttcacca ttattcagcc atggttcaag
    17221 ggtataacga gacagaccaa agcctgccac cgtattgaca tcttcatgca gcaggcctgc
    17281 tttgagcagt tcacgcacca gaaccggtac gccacctgcc gcctggaagt ggttaatatc
    17341 ggccggaccg ttcgggtaga gacgtgccat cagcggtaca acatcagaaa ggtcagagaa
    17401 gtcatcccag ttaatctgaa taccggccgc gcgcgccatc gccaccaggt gcatggtgtg
    17461 gttagtggaa ccaccggtcg ccagcagtgc aacgataccg ttcaccacca ctttctcatc
    17521 gatcatctta ccgatcggca tccattcatt accattaccg gtcatgcgtg taacctgacg
    17581 cgcagcggca gcggtcaaag catcgcgcag cggagaatcc ggatgaacaa aagaagagcc
    17641 tggcaactgc atccccataa attccaccac catctggttg gtgttggcag taccgtagaa
    17701 agtacatgtt cccggcgcat ggtaagacgc ggcttctgac tccagtaagg ccatgcggtc
    17761 cactttacct tcggcataaa gctggcgaat acgcactttt tctttatttg gcaaaccgct
    17821 tgccatcggt ccagacggca caaacaccgc aggcaaatga ccaaacgaca gggctgccat
    17881 cgtcagaccc gggacaatct tgtcgcacac accgaggaac agagcaccat caaacatgtt
    17941 atgggacagc ccgaccgccg cagacatcgc tatcacttcg cggcttagca gcgacaattc
    18001 cattccatcc tgcccctggg tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg
    18061 accaaccgca ttcgcttcat gcagggcttt acgaatgatt tctggatagt gttcataagg
    18121 ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg gcgatattgt tacgcaacat
    18181 gcttttcaaa gaggctttgt cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc
    18241 gcatgccaac tgcgaacgat gaacagtcga cgttttcgct tgttctatcc gggcgagata
    18301 agcagagcga gtctcgcgcg aacgttcaat gattcgattt gttacgcgta acaattgtgg
    18361 attcataaag gctcctgaaa ttgagttgtc agagcaggat gattcacaac gcgtttcatt
    18421 cagaggattt atgactgaaa cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc
    18481 agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt
    18541 tctacaatct gtgcaagatc atgttaccgg taaaataacc ataaaggata agcgcagata
    18601 ttactcaaac tcattccagg aacgaccatc acgggtaatc atcgccaccg aggcaacggg
    18661 tccccaggtt ccggcctgat acggtttcgg cgcatcattg tccatcgccc acgcctcagt
    18721 aatggagtct acccatttcc aggcttcttc cacttcatca cgacgtacaa acagtgcctg
    18781 aataccacgc atggtttcca gcagcagacg ttcataggca tccgccagat gcgtctgatt
    18841 aaaggtttct gaatagctca gatccagctt ggtgatttgc aggttatgtt tgtggtcaag
    18901 gccaggaact ttattcagta cctggatatc cacgccttca tcaggttgca gacggatagt
    18961 cagtttattc tgcggcagat cctgccacga ctctttaaac agattcagtt caggtgtttt
    19021 gaaatagacc acgacttcag aacatttggt cggcagacgt ttaccagtac gcaggtagaa
    19081 tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg atcgccacga aggtttctgt
    19141 attgctgctc ttgttcgcgc cctcttcttc cagatatccc ggcacttttt tgccctgggc
    19201 gaagcccgca gtatattgcc cgcgtacggt tttttcgcgt acgttggagc ggtcgatgcg
    19261 gcgcagggac ttcagtactt tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga
    19321 tggcggagac atcgcaatca tgcagagaat ttgtagcagg tggttctgaa tcatgtcacg
    19381 catctgaccg gctttatcaa aatagcccca gcgcccttcg atccccactt cttctgccac
    19441 ggtaatctca acatgatcaa tggtgcgatt gtcccagtta ttcacaaaca gggagttagc
    19501 aaaacgcagc gccaacaggt tcagcaccgt ttctttacca agatagtggt cgatacggta
    19561 aacctggcac tcctcgaagt attcgccaac ctgatcattg atttcctgcg aggtcgccag
    19621 cgacgtcccc agcggtttct ccatgactac gcgtgccggt ttagcattca gttttgcctc
    19681 gccaagccct ttgcaaattg cgccaaaagt gctgggcggc atggcaaagt agttaatggt
    19741 gatacgattt ttttgatcca gcatcgcgcc gagacggttg aatgcagcag tgtcattgac
    19801 atcgagatta caaaaatcca gacgtgcact cagggtgtcc cataaacctt catcaatggt
    19861 ttctttcatg aaagtttcga gcgcctcgcg gacaactttg gtatatgccg ctttatccca
    19921 gtcagcacgc cctacgccga taatccgggt gtccgggttg agctgaccgg ctttttccag
    19981 ttgatacagg gaaggcagca atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac
    20041 caggtcacag gcctgggctg tttgcgttac cgccatgtca ttctccttaa gttaactaac
    20101 ccggtactta agccagggta tacttgtaat tttcttacgg tgcactgtac tgcttttacg
    20161 agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagt
    20221 gcggcaaaac tgataaaaaa atcatcgttt tccattcggt aaaaatctga cactgatcat
    20281 gttatgaaaa aaaataacaa cttttttatc tgcttttgtc attaacacgg cacacaggcg
    20341 taatatctga caaaactgca tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga
    20401 gtctctttac atcatgaata tgctggaaaa aatccagtct cagctggaac atttgagcaa
    20461 atcagagcgc aaagttgccg aggtcattct ggcttcgccc gataacgcga tccattcgag
    20521 tattgctgct ctggcgctgg aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg
    20581 cagcatggac acgcgcggtt ttcctgattt taaacttcat ctggcacaaa gtctggcgaa
    20641 tggcactccc tatgttaatc gcaatgtcaa tgaagatgac agcgttgaat catacacagg
    20701 gaaaatattt gagtccgcaa tggcaacgct tgatcatgtc cgtcattcac tggataaatc
    20761 tgccatcaac cgcgccgtcg acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt
    20821 aggctcttca gccgccgttg cccacgatgc gatgaataag ttctttcgtt ttaatgttcc
    20881 ggtggtgtac tccgatgata tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg
    20941 agacgtggtg gtgctgattt ctcacactgg aagaacaaaa aatctggtcg agctggcgca
    21001 gctggctcgc gaaaacgacg caatggtgat tgccctcacc tctgcgggta ccccgctcgc
    21061 ccgggaagca acgctggcaa ttaccctcga cgtaccggaa gatactgaca tttatatgcc
    21121 catggtttct cgacttgcac agctgaccgt gatagatgtg ctggcgacag gatttacttt
    21181 gcgacgcggt gcaaaattca gagataactt gaagcgggtc aaggaagcgc tgaaggaatc
    21241 gcgttttgat aagcagttac ttaatttaag tgacgatcgc taaaaacgac tgtcactgtc
    21301 ctaatcttat acgacatccg aatgagatta atttatcgcc atcgcggcgt tatttcattc
    21361 ggatttcatg ttcaagcaac acctggttgt ttcagtcaac ggagtattac atgtccagaa
    21421 ggcttcgcag aacaaaaatc gttaccacgt taggcccagc aacagatcgc gataataatc
    21481 ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat gaacttttct cacggctcgc
    21541 ctgaagatca caaaatgcgc gcggataaag ttcgtgagat tgccgcaaga ctggggcgtc
    21601 atgtggctat tctgggtgac ctccaggggc ccaaaatccg tgtatccacc tttaaagaag
    21661 gcaaagtttt cctcaatatt ggggataaat tcctgctcga cgccaacctg ggtaaaggtg
    21721 aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg
    21781 gtgacatcct gctgctggac gatggtcgcg tccagttaaa agtactggaa gttcagggca
    21841 tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc caacaataaa ggtatcaaca
    21901 aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca gacattaaga
    21961 ctgcggcgtt gattggcgta gattacctgg ctgtctcctt cccacgctgt ggcgaagatc
    22021 tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg
    22081 ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga tgacatcatc ctcgcctctg
    22141 acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg
    22201 gcattcagaa agcgttaatc cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga
    22261 cccagatgat ggagtcaatg attactaacc cgatgccgac gcgtgcagaa gtcatggacg
    22321 tagcaaacgc cgttctggat ggtactgacg ctgtgatgct gtctgcagaa actgccgctg
    22381 ggcagtatcc gtcagaaact gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa
    22441 tcccgagcat caacgtttct aaacaccgtc tggacgttca gttcgacaat gtggaagaag
    22501 ctattgccat gtcagcaatg tacgcagcta accacctgaa aggcgttacg gcgatcatta
    22561 ccatgaccga atcgggtcgt accgcgctga tgacctcccg tatcagctct ggtctaccaa
    22621 ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac tgctctctat cgtggcgtta
    22681 cgccggtgca ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc
    22741 tgctgcgcga taaaggttac ttgatgtctg gtgacctggt gattgtcacc cagggcgacg
    22801 tgatgagtac cgtgggttct actaatacca cgcgtatttt aacggtagag taagtacgtt
    22861 gccggatgcg gcgaaaacgc cacatccggc ctacagttca atgatagttc aacagatttc
    22921 gaatattctg aagcaaactt gaacttatca tcaggcgaag gcctctcctc gcgagaggct
    22981 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    23041 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    23101 cgacttcttc attcatccgc cgcgcaatcg tatgatcatc cgcctctaac agatcatcca
    23161 tcggtgggcg cacctgaatc gtcagacggt gcgtcttgcc atcataaatc ggaaacagcg
    23221 gaacaacgcg cgcacggcac actttcatca aacgaccaat cgcgggcaac gtcgctttat
    23281 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    23341 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    23401 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    23461 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    23521 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgcctca
    23581 tctcttcgat gatctccagc ccttgccagt caacgcgcgg ctgaattttc tccggtccgc
    23641 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    23701 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaaa ttgattaacg
    23761 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    23821 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    23881 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    23941 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    24001 gtagcggcgc aacttgcccc gcaccaaata aaaaaagccg gtactgactt cgtaccggct
    24061 gcgaatggat gttaattaat caaaccgtag ctgcggcaca atctctttgg cctgcgccag
    24121 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    24181 gtttacggcc tgctggttta tccatacttc atagtgcaaa tgcggcccgg ttgaacgtcc
    24241 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    24301 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    24361 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    24421 tggcgtaccc tgcggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gaccggtcac
    24481 cggattagta cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    24541 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcttcagc
    24601 gcggatagcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    24661 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    24721 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    24781 ggttaaaccg gcgtttctgg cgctggcaac aaagctcccc ccgacggtac ctttcagcag
    24841 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    24901 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    24961 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    25021 ggcagcagcc agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    25081 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    25141 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    25201 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    25261 cgtggcatcg cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    25321 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    25381 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    25441 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    25501 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    25561 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    25621 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    25681 aagcggtgtc agtccaaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    25741 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggctaattg
    25801 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    25861 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    25921 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    25981 atcatcgccg tgaatacttt tcatcagcag cggtttcaca tcttctagct gtgcaatcgt
    26041 tacctgtttc gcttctggta atttacttac cggtttttgc ataaacgctt ccatctccgg
    26101 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    26161 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    26221 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    26281 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    26341 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    26401 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    26461 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    26521 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    26581 ctggtaacac ccgatgaagg ggttatcaag cgtaatggaa aactgcgcat cggctatgta
    26641 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    26701 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    26761 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actgttagcg
    26821 cgagcattgt taaatcgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    26881 gtaaatggcc aggtggcgtt atatgacctt attgaccagt tgcgtcgcga actggattgt
    26941 ggcgttttaa tggtctctca cgatctgcat ctggtaatgg caaaaaccga tgaagtgctg
    27001 tgtctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    27061 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    27121 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    27181 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    27241 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    27301 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    27361 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    27421 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggcctg gtggtcgtta
    27481 gtctgatgtc taatattcgt gttgatttga tggcttacct gttcggtgat ttgctggcag
    27541 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
    27601 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    27661 gtgtgaaatt acagcgcgtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    27721 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    27781 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    27841 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    27901 cggtggtgct atgtgcggca ctgttattta ttctcagtat gatgaaaaag caggccagct
    27961 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    28021 gctatctctg gtaggcctgg taagacgcga atagcgtcgc atcaggcaca ctgccaatgt
    28081 cggatgcggt gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    28141 gattccacgc ccgcgtcgtc gccatacgcc cacgcggtgt acgctgcaaa aagccttgct
    28201 gaatcaaata aggttccagc acatcctcaa tggtttcacg ttcttcgcca atggctgccg
    28261 caaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaataact
    28321 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccaac gcctgagcag
    28381 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    28441 gcagacggtt ggcaatgcgc ggcgtaccgc gagctcgacg agcaacttcc agcgcgccgt
    28501 catcactcat ctcaagcccc ataaagcgtg cgctgcgact gacgatatat tgcagatccg
    28561 gcacctgata aaactccaga cgctgcacaa taccaaaacg atcgcgcaac ggtgatgtca
    28621 gcgaacctgc gcgcgtggtt gcaccaatca gggtaaacgg cggcaaatca attttaatgg
    28681 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccggat
    28741 acaacacctc ttccaccact ggcgaaagac ggtggatctc atcaataaac aacacgtcat
    28801 gcggttcaag gttagtgagc atcgctgcca gatcgcccgc cttttccagc accggaccag
    28861 aagtcgtgcg taaattaacg cccatttcat tggcgacgat gttggcaagc gtagttttac
    28921 ccaaccccgg aggaccgaaa atcaacagat gatcgagggc atcgccgcgc agtttcgctg
    28981 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    29041 atttagggcg aatggcgcga tctgccacat cttccggcaa agtggtaccg gcagaaatca
    29101 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    29161 tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cctcttgtgg
    29221 tttatagccc agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    29281 gctggcaggt gatgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    29341 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    29401 cagtttcacc agcgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    29461 cattccggag aggatcgcca gcgccaactt cgggccgacg ccgttggttt tgatcaactc
    29521 tttgaacaat gtgcgctctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    29581 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    29641 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    29701 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    29761 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgaa ggatgaagaa
    29821 aacttgcgag gtgtcccgat gatctgaaaa atggcagagt ataatttatt caacaggtta
    29881 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    29941 cattgcgact cctgaaaaaa taaatttttt atgctattac gtatattcgt atcgatttca
    30001 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    30061 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    30121 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    30181 tcccctttca accatcacca cgctatctcg ttttattaaa cgttcgtcta atcaacgcta
    30241 tgctataaag cgattagacg ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca
    30301 ggttccttcc cggctacgca aactttacca cacgggtttt aaatacgaat cttcccgcca
    30361 gttctgttca aaatttgtct ttgatattta taaagaggcg ctatgtattc cggtgggtga
    30421 aatagagacg tttggacaac tgttaaatag caatccgaat gcaaaactca ctttctggaa
    30481 attctggttc ttaggttcta ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg
    30541 gcatcatccg ggtttggtgt tgattcacgc ggagggagtt gaaacgcctc agcctgaact
    30601 gaccgaggcg gtataactta acgcagtcgc cctctcgcca ggttcagccg cgattcgctc
    30661 atctgcatcg cattctgact gacgtgacaa tgggtaatcg cgatagctaa cgcatcggcg
    30721 gcatccgcct gtggattagc gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg
    30781 cttttttcgg cactaccaat acctaccacc gtttgcttta cctgacgtgc cgcgtattca
    30841 aataccggca attcctgatt caccgccgca acaatcgcca cgccgcgcgc ctggcccagt
    30901 ttcagggctg agtcagcgtt cttcgccata aagacttgtt caatggcgaa ataatcaggc
    30961 tggaactggg tgatgatttc cgtcacgccc gcatagatga gcttcagacg agacggtaaa
    31021 tcatccactt tggtgcgtat gcatccgcta cccaggtagg acagttgcct gcctacctgg
    31081 cgaattacgc catagccggt cacgcgcgaa cccggatcaa tgccgagaat aatagccatc
    31141 acgcgtctcc gttttgctgt ttagcaggcc tcatcagaga gtcgctgcga cctcatcaga
    31201 gatttcaccg ttatggtaaa cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag
    31261 acgcatcagt ttcggtgcgg tttctgcatc catatcagct ttggtggacg ggatcatgga
    31321 aacttccgcg ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgca ctttacccat
    31381 ttcttcccat gcagtgtaga catcaatcgc gccgtcatca taggtcacaa cgtcttcagc
    31441 acctgcttcc agagctgctt ccatgatggt gtcttcatcg cctttctcga aggagatcac
    31501 gccttttttg ctgaacagat aggctacgga accatctgta ccgaggttac cgccacattt
    31561 gctgaatgca tgacgcactt cagcaacggt acggttgcgg ttgtcagaca gacattcaat
    31621 catgattgcc gtgccgccag gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc
    31681 atcatcaccg cccacaccac gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt
    31741 agacagtgct ttatccaccg ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc
    31801 cagcttagcc gcggttacca gctcacgaat gattttagtg aagattttac cgcgcttagc
    31861 atcctgcgca gctttacgat gtctggtgtt ggcccattta ctatgacctg ccataaaaat
    31921 atctccagat agccctgcct gttcaggcag cgttaattac aaactgttca atcgcctgcc
    31981 ggttgctcca ggacttagtg agcgccgccg cagcaggcgc atcaagccac ttgtaagcca
    32041 gatgttcagt gaaaacgatc tgccgctcgt gcggaagcgc aagacagaac catgattccg
    32101 tattacgcgt cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta
    32161 ccgtgcgctg acagtcaatt aaggtcagtt gttcagcgac aacatcaatg gtgacctctt
    32221 cctttacttc gcgcatggca gcttgcggcg cggtttcacc ctcttccacg ctgccggtta
    32281 ccgactgcca gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt
    32341 gtgcatagat gaccactaag atcgaaacgg gacgcttata cactttatcc tccacgctgc
    32401 ctcttcgttg actgccttcg ctcatcccat tcacatagtt atctatgctc atgggagttc
    32461 actcagttgc cgccttgatg caacgcgaat gatttcgtgt atttgagtca tatcagttat
    32521 tctcagcctt cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc
    32581 tcggtgcttc agtcatcaga cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac
    32641 ggatattgtc ggtgccggtc agcagcatgg tcagacggtc aagaccgaat gccagacctg
    32701 cgtgcggcgg agtaccgtat ttcagagcgt cgagcaggaa gccgaatttc tcgcgctgtt
    32761 cctcttcgtt gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac
    32821 gtactgaacc accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg
    32881 cattttccgg tgcagctttc agttctgcag cagtcatgtc tttcggtgag gtgaacggat
    32941 ggtgcattgc cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc
    33001 acagcggtgc ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggcgca
    33061 gtgcgcccat cgcgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc
    33121 catcttgcgc ggcagtacga tccaggatgg cttcgatgat ttctgcatta aggaacttcg
    33181 ctaccgggct gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca
    33241 gacctttcgc gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca
    33301 gcgatgcgcc gcccggaacg cgcagagcgg caacgcgacc tttcggatcg ttcgccggac
    33361 ctgcaaatac tgcaaactca acagatttca gcagatcagc aacgtcggtc agttccatcg
    33421 ggttacgcag atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta
    33481 ccgggaaatc gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt
    33541 ccatcacttc acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa
    33601 attcaggctg acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt
    33661 aacggtcaaa accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg
    33721 cgtagaattt acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg
    33781 tggctttggt cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc
    33841 gcaccaggct ggtgatttta gcgcgggttt tcaggcgctg cgccatttcc gggcgacgca
    33901 ggtcgaggta gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg
    33961 gcagaacatc tgcgcggttg atgatagtca gcgaggacgc cagcacttca atttcgccgg
    34021 tcgccatatc gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa
    34081 tgcagaactc attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga
    34141 aaaatacctg cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat
    34201 cacgacgacg gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga
    34261 gctgtccaca atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc
    34321 cctgctgcgc aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag
    34381 gcggctatta tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct
    34441 cgttaccact ttatatcggg cctgaaatca gactctacgc cagtttgcta taaaggtgtt
    34501 gcccgaactc ataaaaatta acaaaatttg tcgttccgcc atcggctaat cgcattaagg
    34561 tgtgaggcac gattttgttt tttcaggagt cagcatgctt gaacttaatg ctaaaaccac
    34621 tgcgttggtg gtgatcgatt tacaagaagg catcttacct tttgccggtg gtccacatac
    34681 tgccgatgag gtagttaatc gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca
    34741 gcccgtgttt ctggtgcgcg ttggctggtc tgccgatcac gccgaagcat taaaacagcc
    34801 ggttgatgcc ccctcccccg ctaaagtgtt gcccgaaaac tggtggcaac atccagctgc
    34861 attaggtgca actgacagtg atatcgaaat catcaaacgt cagtggggtg cgttttacgg
    34921 tacggatctg gagttgcaac tgcgccgccg aggtatcgat acaatagtgt tatgtgggat
    34981 ctcgaccaat atcggtgttg aatccaccgc ccgcaatgcc tgggaacttg gctttaatct
    35041 ggtgattgcc gaagacgcct gtagtgccgc cagcgccgag cagcacaata acagcatcaa
    35101 ccatatctac ccacgcatcg cccgtgtgcg tagcgtggaa gagatccttc acgcgttatg
    35161 atttacatcg gtctaccgca atggtcgcat cctaaatggg tgcggttggg aatcaccagc
    35221 cttgaagagt atgcccgcca ctttaactgc gtggagggca acaccacgct ttacgccctg
    35281 ccgaaacccg aggttgtcct gcgctggcgt gaacagacca cagatgactt ccgcttctgt
    35341 tttaagtttc cggcgaccat ttcgcatcag gcagcattac gacattgcga tgatttagtg
    35401 actgaatttt tgacccgcat gtcaccgttg gctccgcgca ttgggcaata ctggctgcaa
    35461 ctgcctgcca cattcggccc acgggagctg cctgcgcttt ggcattttct cgattctctc
    35521 cctggcgaat ttaattatgg tgtggaagtc cgccatccac agtttttcgc caaaggggaa
    35581 gaggaacaaa cgcttaatcg cggtttacat cagcgcggcg ttaatcgggt gattttagac
    35641 agccgcccgg ttcatgcagc acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa
    35701 aaacctaaag ttccggtaca tgctgtactg acggcgacaa atccgctgat ccgttttatc
    35761 ggtagtgatg atatgacgca aaaccgggaa ttatttcagg tctggttaca aaaattagcg
    35821 cagtggcatc agaccactac gccttatctt tttttacata cgccagacat tgcccaggcc
    35881 ccggaactgg tacataccct gtgggaagac ttacgtaaaa cgcttccaga gatcggagca
    35941 gttccggcta ttccacagca atcttctctt ttctgaattt gccacctatc atagacaggt
    36001 gccatcggcc attttaaagg gagtttgtat ggtaagcgcg ctgtatgccg ttttaagtgc
    36061 gttgttatta atgaagttct cttttgatgt cgttcgcctg cgaatgcagt accgcgttgc
    36121 ctatggtgac ggtggtttta gcgaactgca aagcgctatt cgcattcatg gtaacgcggt
    36181 ggaatatatt cccatcgcga ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac
    36241 ctggatggtg catatttgcg gcatcgtttt gcttgctggt cgtctgatgc attattacgg
    36301 ttttcatcac cgtctgttcc gctggcgacg ttccggcatg agcgccacct ggtgtgcgct
    36361 gttgctgatg gtgctggcga atctttggta tatgccctgg gagttggttt tctccctgcg
    36421 ttagcgcaca atacgccact ttctttttcc cggattttta cgttatgtct caccgcgaca
    36481 cgctattttc tgcccctatc gccagactgg gcgactggac ctttgatgaa cgggtagctg
    36541 aagtcttccc ggatatgatc cagcgttccg ttcccggcta ttccaatatt atttccatga
    36601 ttggtatgtt agccgagcgc ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt
    36661 ctctgggcgc ggcgacgctc tcggtgcgtc gcaacattca tcatgataat tgcaaaatta
    36721 ttgccatcga caactccccg gcgatgattg aacgctgccg tcgtcatatt gacgcctata
    36781 aagcccctac gccagtagac gtcattgaag gtgatattcg cgatatcgcc attgaaaacg
    36841 catcgatggt ggtgctgaat tttaccctgc aattcctgga accttccgag cgccaggcat
    36901 tactggataa aatttatcaa ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat
    36961 tcagtttcga agatgccaaa gttggtgaac tgttgttcaa catgcaccac gactttaaac
    37021 gtgccaacgg ttacagcgaa ctggagatca gccagaagcg cagcatgctg gaaaacgtga
    37081 tgctgaccga ttccgtggaa acccataaag cacgcctgca taaagccggt tttgagcata
    37141 gcgagctgtg gttccagtgc tttaactttg gttcactggt ggcattaaaa gcagaggacg
    37201 ctgcatgatc gactttggta acttttattc tctgattgcc aaaaatcatc tttcacactg
    37261 gctcgaaacg ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc acgggctgtt
    37321 taagcagtgg tccaacgcgg tggaatttct gcctgaaatt aaaccgtatc gtctggattt
    37381 attgcatagc gtaaccgccg aaagcgaaga gccactgagc accgggcaaa ttaaacgcat
    37441 tgaaacgctt atgcggaacc tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt
    37501 caacatcgat accgaatggc gttccgactg gaaatgggat cgtgttctgc cccatctttc
    37561 tgatttaacc ggacgcacca ttctggatgt cggctgcggc agcggttatc acatgtggcg
    37621 catgattggc gcaggcgcgc acctcgcggt ggggatcgac cctacgcaac tgtttctgtg
    37681 ccagtttgaa gcggtgcgta aactactggg taacgatcag cgcgcacatt tgataccgtt
    37741 aggtattgaa cagcttccgg cactgaaagc ctttgatacc gtcttttcga tgggcgtgct
    37801 ttaccatcgc cgttcgccgc tggagcatct ctggcagttg aaagatcaac tggtgaatga
    37861 gggtgaactg gtgctggaaa cgctggttat tgatggtgat gagaacacgg tgctggtacc
    37921 aggcgatcgt tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa
    37981 aaactggctg aagaagtgtg gttttgtaga tatccgcatt gtggatgtgt gcgttaccac
    38041 tactgaagag cagcgacgca ctgaatggat ggttaccgaa tctctgtccg atttcctcga
    38101 cccacatgat ccgagtaaga cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat
    38161 tgcgcgcaag ccgtaaaggt ctggtattac tgccggatgc ggcgtgaatg ccttatccgg
    38221 cctacaaacc cttgctaatt caatctattg cagggactat gtaggcctga taagcatagc
    38281 gcatcaggca gttttacgtt tgcataacct cagcgcccgt ttccgggcgc tattcacttc
    38341 ttacgcctga acagcaggct tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt
    38401 ttcgatatac accagcgcag agttaccggc acaggcattt gccagctgcg agctggggat
    38461 atccgccgtt aacacgttcg cactgccgtt tttacataag ccattttcca gatctggcca
    38521 tgcaccttca tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcacctgt
    38581 tagtatctga ccacgtttgt tccagacacg caccagatcg ccattcgcaa taccaaatcg
    38641 agtagcatct tcggtgtgaa tagtgatcgg ttcacgatcg gcaaccgcat attttttacg
    38701 cagtcccgca tagttaagct gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg
    38761 caactgcttc tcgtcggcgg tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc
    38821 cgggcaatcc ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct
    38881 tggcgtaccc agcgcatttt tcaccggatc ggcgcggaaa tcaccataac gaacgtactg
    38941 ttcgttcttc tcgctgctgc gcatttcgat cagtttattt tgctgccaga aggcattaaa
    39001 catcggcata gtcacgcgtt gcgcacgagc acctttctga gcggcatcat agaaaaattt
    39061 cagccacgcc atttcatctt taccttcggt atagatctct tttccgccag gtttcagtaa
    39121 ttcagcaaga tcggcaaata cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc
    39181 ctgcttcatc ggcacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg
    39241 ctcaaacgat gtggtgatcg gtaatacgat atctgcatgt ttagcagctg cggtccagta
    39301 gcattcagaa acgacgatca tctccggttt ctgccatgct ttgatcagac gattagtatc
    39361 ctggtgatgg gtaaagttac cgccacccgc ccaccagatc attttgatat tcggatacgt
    39421 ttgttcttta ccgttatgct gatatttgcc accaggattt tccagcgcat cgacgatacg
    39481 ggcaacagga atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat
    39541 cgccgcagac atttccggca gcacgccgcc aacacgcgtc ggattgccac cgttggagta
    39601 atgataagag aaaccaaaac cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag
    39661 cgtcaccagc atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagccagc
    39721 catcagcatt gtgcgattcg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg
    39781 tacgccagta atttccgccg cccaggccgc gctcttcgga gtattatcgc tcttacctgt
    39841 cagatactct tcaaactgcg gatacccggt ggtgtatttt tcaaggaaca ctttatcgtg
    39901 tttaccttgt gtcatcaggg tatgcgcaat ccctaacatc agtgccacgt cggtgcccat
    39961 attcggcgcg atccaggtgg cattatcacc aaagaactcg atggtttcgg agcggatagg
    40021 atcaatggca atcactggtt tgccagattt tttcagctga tggaagtatt ccagcccttg
    40081 ctcatcggta ctgctccagg caatttttaa ggtattcagc gggttcattc cccacagcac
    40141 cacaacctgg ctgttttcca gaatcagcgg ccaggaggtc tgctgttcat acacctctac
    40201 agaaccgacc acatgcggca tgatcacctg tgccgccccg gttgaataat cgccgctatg
    40261 cccggaataa ccgcccgcca ggttcatata acgttgcagt aaggtttgcg ctttatgcaa
    40321 cacgccagaa gagcgccagc cgtatgatcc ggcaaaaata gccgatggtc cgttagcttt
    40381 gcgaatgcga tcgtgttgct catgaatcag ttttaatgct tgttcccagc tcacctgtac
    40441 ataggtatct tcgccacgac ctttcgtcgg ttgcagtgga ttatcgagat agctttttct
    40501 caccatcgga tgctgaatac gcgtcgtggt gtgtacctga tccgccgccg tagactgtaa
    40561 ggaattcggt atggttttcg ccagcgcgcc tgttgaagaa acaatcttgc cgtccttcac
    40621 ttctacgttc atcgctcccc aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc
    40681 ccatgcgggg agcggtgctg ccgatgtcac caccagcgct ccagcggcaa taccgctgtg
    40741 tttaataaat tcacgtcttg ttaatgtcat aacttcctcc ctgatcaacg aggatcactg
    40801 tttctcggta atatctttgg cgttgtactg gaaataccgc gttaaaatgt ccagttcgtt
    40861 ttcgctcatg ctggttcgcg cacccattcc tttggcaatg gacggccatg cattgacggt
    40921 gtaatggtca gcggcaatag gcgcatggca accagcgcaa taggtatcgt caagtttttc
    40981 agcgtattgc catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa
    41041 agacgcctga cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag
    41101 caccttgata ccttcttcac ttaatgtggc tagcgccagc cgttgacctg ccgccaggta
    41161 gagcgtgttt tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc
    41221 actggcatca acgacggtga gatcggtacc aggattcacg gtagccagct cgcctatgcg
    41281 agaagttttg aaaggataaa tatgtgcgcc attagtcact gaagtagcgg cctgactttc
    41341 cagctcatgc gccgcgttgt catccatttt tatttctggc ggaaaatggg caatgccttt
    41401 atgacaatcg atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga
    41461 ttcactttgc gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga
    41521 gtcagttgtt tttaattctt tccatactgt ttcggccatt tcctggcgat gagtttcgaa
    41581 cttatcgtca ctgtctattt tgccgctaac aaattcatga taaatatctt tagatgcttt
    41641 taatttagca aataaataat ccatccctga ctttggaata tggcaatctg cacattccgc
    41701 acgtatccct ttctggttcg aaaagtggac agttccctga tattcctcaa aaggtttact
    41761 catcgagtgg caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc
    41821 cagcaataac ccgccgccac caaccacaac cgctatcagc aaaaacaata acccaatgcg
    41881 ttttttccct cgcatagatt cctctaaata aattccagaa caccgaaatc attgtataat
    41941 taaatacaca acg
//
LOCUS       DAZFRN010000024        41807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFRN010000024 DAZFRN010000000
VERSION     DAZFRN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.024"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(116..2812)
                     /gene="mgtA"
                     /locus_tag="ACHKUB_001257"
     CDS             complement(116..2812)
                     /gene="mgtA"
                     /locus_tag="ACHKUB_001257"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO6654299.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(2953..3006)
                     /gene="mgtL"
                     /locus_tag="ACHKUB_001258"
     CDS             complement(2953..3006)
                     /gene="mgtL"
                     /locus_tag="ACHKUB_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO6654300.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            3191..4138
                     /gene="treR"
                     /locus_tag="ACHKUB_001259"
     CDS             3191..4138
                     /gene="treR"
                     /locus_tag="ACHKUB_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO6654301.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            4257..5678
                     /gene="treB"
                     /locus_tag="ACHKUB_001260"
     CDS             4257..5678
                     /gene="treB"
                     /locus_tag="ACHKUB_001260"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO6654302.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            5728..7383
                     /gene="treC"
                     /locus_tag="ACHKUB_001261"
     CDS             5728..7383
                     /gene="treC"
                     /locus_tag="ACHKUB_001261"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO6654303.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NNGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALT
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRG
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            7777..9915
                     /gene="nrdD"
                     /locus_tag="ACHKUB_001262"
     CDS             7777..9915
                     /gene="nrdD"
                     /locus_tag="ACHKUB_001262"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO6654304.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            10100..10564
                     /gene="nrdG"
                     /locus_tag="ACHKUB_001263"
     CDS             10100..10564
                     /gene="nrdG"
                     /locus_tag="ACHKUB_001263"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO6654305.1"
                     /translation="MNFHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQMVHHLR"
     gene            complement(10565..10855)
                     /locus_tag="ACHKUB_001264"
     CDS             complement(10565..10855)
                     /locus_tag="ACHKUB_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6654306.1"
                     /translation="MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKL
                     RDLPDCYKIKLRTLGYRLVYQVNDKELLVLVISIGKRENSAVYEDATKRLDE"
     gene            complement(10845..11087)
                     /locus_tag="ACHKUB_001265"
     CDS             complement(10845..11087)
                     /locus_tag="ACHKUB_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFO6654307.1"
                     /translation="MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRM
                     PVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL"
     gene            complement(11279..11665)
                     /gene="cybC"
                     /locus_tag="ACHKUB_001266"
     CDS             complement(11279..11665)
                     /gene="cybC"
                     /locus_tag="ACHKUB_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO6654308.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(11848..13200)
                     /gene="pmbA"
                     /locus_tag="ACHKUB_001267"
     CDS             complement(11848..13200)
                     /gene="pmbA"
                     /locus_tag="ACHKUB_001267"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO6654309.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            13294..13845
                     /gene="yjgA"
                     /locus_tag="ACHKUB_001268"
     CDS             13294..13845
                     /gene="yjgA"
                     /locus_tag="ACHKUB_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO6654310.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(13990..15363)
                     /gene="mpl"
                     /locus_tag="ACHKUB_001269"
     CDS             complement(13990..15363)
                     /gene="mpl"
                     /locus_tag="ACHKUB_001269"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO6654311.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            15539..16537
                     /gene="fbp"
                     /locus_tag="ACHKUB_001270"
     CDS             15539..16537
                     /gene="fbp"
                     /locus_tag="ACHKUB_001270"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO6654312.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(16570..17565)
                     /gene="yjfF"
                     /locus_tag="ACHKUB_001271"
     CDS             complement(16570..17565)
                     /gene="yjfF"
                     /locus_tag="ACHKUB_001271"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO6654313.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(17552..18574)
                     /gene="ytfT"
                     /locus_tag="ACHKUB_001272"
     CDS             complement(17552..18574)
                     /gene="ytfT"
                     /locus_tag="ACHKUB_001272"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO6654314.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(18588..20090)
                     /gene="ytfR"
                     /locus_tag="ACHKUB_001273"
     CDS             complement(18588..20090)
                     /gene="ytfR"
                     /locus_tag="ACHKUB_001273"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO6654315.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPENRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(20400..21356)
                     /gene="ytfQ"
                     /locus_tag="ACHKUB_001274"
     CDS             complement(20400..21356)
                     /gene="ytfQ"
                     /locus_tag="ACHKUB_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO6654316.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            21666..22196
                     /gene="ppa"
                     /locus_tag="ACHKUB_001275"
     CDS             21666..22196
                     /gene="ppa"
                     /locus_tag="ACHKUB_001275"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO6654317.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(22576..22917)
                     /gene="ytfP"
                     /locus_tag="ACHKUB_001276"
     CDS             complement(22576..22917)
                     /gene="ytfP"
                     /locus_tag="ACHKUB_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO6654318.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKI
                     IESGDWLDRDK"
     gene            complement(22920..26699)
                     /gene="tamB"
                     /locus_tag="ACHKUB_001277"
     CDS             complement(22920..26699)
                     /gene="tamB"
                     /locus_tag="ACHKUB_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO6654319.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTAVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVNTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPQTTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(26696..28429)
                     /gene="tamA"
                     /locus_tag="ACHKUB_001278"
     CDS             complement(26696..28429)
                     /gene="tamA"
                     /locus_tag="ACHKUB_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO6654320.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            28635..29273
                     /gene="msrA"
                     /locus_tag="ACHKUB_001279"
     CDS             28635..29273
                     /gene="msrA"
                     /locus_tag="ACHKUB_001279"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO6654321.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            29596..30939
                     /gene="paeA"
                     /locus_tag="ACHKUB_001280"
     CDS             29596..30939
                     /gene="paeA"
                     /locus_tag="ACHKUB_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO6654322.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(31001..31207)
                     /gene="ytfK"
                     /locus_tag="ACHKUB_001281"
     CDS             complement(31001..31207)
                     /gene="ytfK"
                     /locus_tag="ACHKUB_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO6654323.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            31532..32089
                     /gene="ytfJ"
                     /locus_tag="ACHKUB_001282"
     CDS             31532..32089
                     /gene="ytfJ"
                     /locus_tag="ACHKUB_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6654324.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(32079..32819)
                     /gene="cysQ"
                     /locus_tag="ACHKUB_001283"
     CDS             complement(32079..32819)
                     /gene="cysQ"
                     /locus_tag="ACHKUB_001283"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO6654325.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            33009..34952
                     /gene="cpdB"
                     /locus_tag="ACHKUB_001284"
     CDS             33009..34952
                     /gene="cpdB"
                     /locus_tag="ACHKUB_001284"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO6654326.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GKCQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(35081..35461)
                     /gene="ytfH"
                     /locus_tag="ACHKUB_001285"
     CDS             complement(35081..35461)
                     /gene="ytfH"
                     /locus_tag="ACHKUB_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO6654327.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            35550..36410
                     /gene="qorB"
                     /locus_tag="ACHKUB_001286"
     CDS             35550..36410
                     /gene="qorB"
                     /locus_tag="ACHKUB_001286"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6654328.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            36518..37483
                     /gene="ytfF"
                     /locus_tag="ACHKUB_001287"
     CDS             36518..37483
                     /gene="ytfF"
                     /locus_tag="ACHKUB_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6654329.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            37591..38253
                     /gene="ytfE"
                     /locus_tag="ACHKUB_001288"
     CDS             37591..38253
                     /gene="ytfE"
                     /locus_tag="ACHKUB_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO6654330.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLSRAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(38298..39710)
                     /gene="cycA"
                     /locus_tag="ACHKUB_001289"
                     /gene_synonym="dagA"
     CDS             complement(38298..39710)
                     /gene="cycA"
                     /locus_tag="ACHKUB_001289"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO6654331.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(40019..40639)
                     /gene="fklB"
                     /locus_tag="ACHKUB_001290"
     CDS             complement(40019..40639)
                     /gene="fklB"
                     /locus_tag="ACHKUB_001290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6654332.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDDTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            40858..41496
                     /gene="ytfB"
                     /locus_tag="ACHKUB_001291"
     CDS             40858..41496
                     /gene="ytfB"
                     /locus_tag="ACHKUB_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO6654333.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(41480..41587)
                     /locus_tag="ACHKUB_001292"
                     /pseudo
     CDS             complement(41480..41587)
                     /locus_tag="ACHKUB_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(41631..>41807)
                     /locus_tag="ACHKUB_001293"
     CDS             complement(41631..>41807)
                     /locus_tag="ACHKUB_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6654334.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
BASE COUNT        10066 a        11304 c        10555 g         9882 t
ORIGIN      
        1 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
       61 acaaagctta ctttgtctgc aatctgaatc ggggctatcg tgcccagttt attctttact
      121 gccagccgta acgacggcta taaaatcctt tcaccaactg agttaatgtc atataccctg
      181 ccaaaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagc tgcagataac
      241 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
      301 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
      361 caatcagcgt ttgcgacagt aagcccacca caaaccatcc cgactggaac agcgtttgcg
      421 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
      481 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
      541 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
      601 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
      661 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
      721 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
      781 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
      841 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
      901 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
      961 tcaggcgggc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     1021 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     1081 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtgattcca ctcgctttta
     1141 atgccttcag tgccggagcg gttgtctctt ttggcggatc aaggaaagca atatatcctt
     1201 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     1261 tcgtcgccac cgcaaccacg cgcagcccct gacgattaag cgtatcagta acccgcttaa
     1321 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     1381 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     1441 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     1501 gccaacgact ggccagcgag cgcgctgact cttcatccgt accttcgagc accgctgtat
     1561 cgagcaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     1621 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     1681 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     1741 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     1801 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     1861 ccgcttccca ccagtcgcct ttggtgtaac cattgattaa cagcaccacc ggagccatca
     1921 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccttgctga aaggcattcg
     1981 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggta ttggcacctg
     2041 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     2101 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttctacgg
     2161 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     2221 gtaaatctgc cgggatcata tcccccgccg ccagttttat tatatcgccg ggcaccagct
     2281 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     2341 tcgctgtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     2401 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     2461 aatcttccgt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aaggggttgc
     2521 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     2581 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     2641 cgaacgtttt ccataattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     2701 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     2761 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     2821 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     2881 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtaacgccgt
     2941 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     3001 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     3061 attcgtagca tctatggcga tataatacct gctggcaact aaaccaacga taaaccagac
     3121 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     3181 ggacaacagg atgcaaaatc ggctgaccat caaagacatc gcacgcttaa gcggcgtggg
     3241 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     3301 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     3361 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgcctggatt cgttgtcaga
     3421 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     3481 gatggaaagt cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     3541 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     3601 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggttttgctt cggtctgtta
     3661 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     3721 tatcagttat ctcggcgtgc cgcatagtga cgtgacgacc gggaagcgac gtcacgaagc
     3781 ctacctggcg ttctgcaaag cgcataaact gaatcccgtt gccgctctgc caggccttgc
     3841 tatgaagcaa ggctatgaga acgtagcaaa agtgattacg cctgaaacta ccgccttact
     3901 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     3961 caccttgcaa ctggcgagcg tcggtaatac gccattaatg aaattccttc atccggagat
     4021 cgtcaccgta gatcctggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     4081 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     4141 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     4201 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     4261 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actagtcggc gggcgcggca
     4321 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     4381 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     4441 ggcaatttca ggtggtgatt ggcaccaatg tgggtgatta ctatcaagca ctaatagcgt
     4501 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     4561 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     4621 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     4681 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     4741 tgatcggtga agcgatcttc ttctacctgc cggtcggtat ttgctggtca gcggtgaaaa
     4801 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     4861 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     4921 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     4981 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     5041 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     5101 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     5161 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     5221 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     5281 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     5341 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     5401 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
     5461 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
     5521 gtatcggcgt aggcggcctg ccgggaattc tctcgattca accgagctac tggcaggtgt
     5581 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
     5641 agcggaaata ccgcctgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
     5701 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
     5761 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
     5821 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
     5881 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
     5941 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
     6001 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
     6061 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
     6121 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
     6181 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
     6241 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
     6301 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
     6361 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
     6421 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
     6481 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
     6541 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
     6601 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
     6661 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
     6721 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
     6781 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggtat gaccaacccg
     6841 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
     6901 cgcaacaatg ggcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
     6961 aacagtcgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
     7021 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctgaccgat
     7081 gagtcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccgtt
     7141 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
     7201 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtgg gatccaaccc
     7261 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
     7321 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
     7381 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
     7441 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
     7501 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagttc aaacatcctt
     7561 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
     7621 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
     7681 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
     7741 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
     7801 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
     7861 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgctgcggt tgtcagcgag
     7921 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
     7981 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
     8041 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
     8101 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
     8161 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
     8221 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
     8281 ttcttcccga tgttcaactg catgttgatt gacctgaaag gcatgctgac ccagggcttt
     8341 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgctgtaact
     8401 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
     8461 gatgaagtgc tggcaccgtt tgtcaccgcc agctacaaca aacatcgcaa gaccgcagaa
     8521 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccattaa agagtgctac
     8581 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
     8641 ccgtttgtaa cctttggttt tggtctgggc accagctggg aatcgcgcct gattcaggaa
     8701 tctatcctgc gtaaccgtat cgcaggcctt ggtaaaaacc gtaaaactgc ggtgttcccg
     8761 aaactggtgt ttgcgattcg cgatggtctg aaccataaaa aaggcgatcc gaactacgac
     8821 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
     8881 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
     8941 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
     9001 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
     9061 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
     9121 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
     9181 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
     9241 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
     9301 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg ccaggcagtg
     9361 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
     9421 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
     9481 gataaaggtt actacaccaa cagtttccac ctcgatgtgg agaagaaggt gaacccgtac
     9541 gacaagatcg actttgaagc gccttacccg ccgctagcga acggtggttt catttgctac
     9601 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
     9661 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
     9721 tttaccggtg agttcgagtg caccagcaaa ggcttcacct gcccgaaatg tggtaaccat
     9781 gacgcctccc gtgtgtcggt gactcgccgc gtgtgcggat atttaggtag cccggatgca
     9841 cgtccgttta acgctggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
     9901 gggcagatag gttaatcctt ccccctcacc ctaaccctct ccctcaaggg agagggtacc
     9961 gttccgtgcc ccttcctgat tcccgacgta atgaattgcc tctggccagt tggtgtcgtc
    10021 gcaaggtggc aaatgcgtag catcggggga cttcgcccgt acgcaccaga gcagtcatgg
    10081 cgtcaattga gaagtgaaaa tgaactttca tcagtactat cctgtcgaca tcgtcaacgg
    10141 ccccggcact cgctgtaccc tgtttgtctc cgggtgtgtt catgaatgcc ccggttgcta
    10201 taacaaaagc acatggcggg taaattctgg tcagccattt accaaagcaa tggaagacca
    10261 gatcattaac gatctgaatg acactcgtat caaacgccag ggtatttccc tctccggcgg
    10321 cgatccgctg catccgcaaa acgtgccgga tattctgaaa ctggtacaac gcatccgcgc
    10381 cgaatgtccg ggtaaagaca tttgggtgtg gacaggctat aaactcgacg aactcaacgc
    10441 tgcgcaaatg caggttgttg atttgattaa cgtgctggtc gacggcaaat ttgtgcagga
    10501 tttaaaagac ccttccctga tctggcgcgg cagcagcaat cagatggtgc atcatttgcg
    10561 ttaatcactc atccagtcgt ttagtcgcgt cttcataaac ggctgagttc tctctcttac
    10621 caatagatat caccaacacg agcaactctt tatcgttaac ctgataaacc aggcgatatc
    10681 ccagtgtgcg cagcttaatt ttgtagcagt ccggtaaatc ccgcagttta gccgatggat
    10741 tgcgcggcct ttttagcaca tcctcaagct tttttttaaa ttgttcccgg actgtcacgc
    10801 ccagtttttg ccactctttc agcgcccgcg gatcaaaagc cagctcatag ttcatccagt
    10861 cggaccttaa tggcctcttg tgggttggcc agtcgttccc tgacagtgcg taacaaggct
    10921 tcttcactat cactgacagt aagcgttttt accggcattc gtccattttc cgcaacgtac
    10981 tgaaacagca gccgcactgc ttcagtaggc gtgatattta atttttccag caccgcataa
    11041 gcctcatttt tcagtttgtc atccaggcgg acgttaagtg tggccattat gcctccggta
    11101 attatgaaaa gtgtaatgca ttatagcatt acatatgcat tacattcatt tctggaaaac
    11161 gcctcgcaaa agtgaggacg acacgcagga atatgccatg gtgaagatag atattgcggt
    11221 gattttacga agggagaaga aagcgttggg cggtaacgtt tcaccgccca ataaggaatt
    11281 aacgatactt ctggtgatag gcgttgcggg tcgttttcag ttgctctgcg gcagcctgcg
    11341 cttcttttac tttaccttca tttgccagct tcagcgcgtc gtcaatctga ccgaccagaa
    11401 tatcgaaacc gtgacggaag tctttcattt ccgggctgtc cggtgattta tcttcgagct
    11461 tcggcggcgt tgctttttgc gcatccagcg ctgcggcgcg catcttcgtt aacgcgtctt
    11521 tgacttgcgc cgcgttatcc gctttttcga tcacttttaa attgtcgttg agggtttcca
    11581 tattgtcttc aagatcagcg gcaaacgacg cagaactgaa taccaacgag gagactgcaa
    11641 gaatagctaa caggctttta cgcattgctc acttcctttt ttatttacac ttcatccttc
    11701 acactgcctc atcgacggct acgctcattc gcccgggtca cgtactgcga gtacgctccc
    11761 cggccctcat tcactttccg tctcgatgca gtgcgaatga ttttgtgtaa ttttttagtt
    11821 tatagcgcgg caggtcgcgc cagtttttta ctgcccggcg attttcatct ccggcaacag
    11881 cacagaacca cactgtatat tactgcgtgt ttcaatatcg ttaccgacgg tgacaatatt
    11941 gcgccacata tcttttaaat tacctgcgat ggtgatttca ctaacgggat actgaatttc
    12001 gccattctct acccagaagc ccgctgcacc acgggaataa tcaccggtaa tggcactcac
    12061 gccctggccc atcagctccg tcaccaccag cccggtgccc atctctttga gcatttgttc
    12121 gaagcttagc ccttgtccgg caatccgcca gttgtgaata ccgcccgcat gtccggtgct
    12181 tttcagcccc agtttccgcg ccgagtagct ggtcagcagc cactgagtca ggatgccatc
    12241 tttaataata tcgcgacgct cggtgcgcac accttcgctg tcgaatggcg tcgacgccag
    12301 ccctttcagc agatgcggat gctcttcaat ggtcagccag tccggcagaa tttgtttacc
    12361 cagcgaatcc agcagaaagg tagatttacg gtaaaccgag ccacccgcta tcgcccccac
    12421 cagatggcca aaaagcccgg ttgccacttc attggcaaaa atgactggcg ctttcatggt
    12481 ggagagttta cgcggtgaca ggcgcgataa agtacggcga gcacagtcgg ccccaaccca
    12541 ctctggcgtt tgcagatcgc tcatcgcgcg gccaatggtg taggcgtaat cacgctccat
    12601 atcgccattt tcttcggcaa ttacacagct ggagagcgaa tgacgcgttg agcagtaacc
    12661 ctgcaacatg ccgtggctgt tgccaaaaac tttgacaccg tagtggctgt taaaactgcc
    12721 gccttcggta ttggtaatgc gtttgtccgc ctgcaatgcc gcctgttctg cgcgggccgc
    12781 cagttcaatg gcttcatccg gggaaacttc agcagggtgg aacaagtcga gatctggtgc
    12841 gtcaaaggcc agcagctctt tgtctgccag cccggcacag ggatctggcg aggtataacg
    12901 ggcaatatcc agcgccgcct gtacagtacg ggcaatggcc tgcgggctta aatcggtgga
    12961 tgatgcgcta cctttgcggt tctggtgata aacagtgatc cccagcgcgc catcactatt
    13021 gaattcaaca ttctccactt caccataacg cgtgcttacg ctaatgccgg tggtcttgct
    13081 gacggcaact tccgcaccgt ccgatttgcc tgaggccaac tccagcgcag tggaaactgc
    13141 ttcttccaga atcttgcgct gcgcttcaac ttgagagatt actttcattg caagtgccat
    13201 aatgtagaga gtttctctga agtctaacag agaaccgttt ttcagtgcgc atcttaactg
    13261 gtaacattag cctctttttt taaggagcct gagatgacta agcagcccga agactggctc
    13321 gacgacgttc ccggtgatga catcgaagac gaagacgatg aaattatctg ggtcagtaaa
    13381 agtgaaatta aacgtgatgc cgaggagcta aaacgccttg gcgcggaaat tgttgatctg
    13441 gggaaaaacg cgctggataa gatcccgtta gatgcggatc tgcgcgcggc tattgagctc
    13501 gcccagcgta ttaagatgga aggtcgccgc cgccagttgc agctcatcgg taagatgctg
    13561 cgccagcgcg atgttgaacc gatccgtcag gcgctggata agctaaaaaa ccgtcacaac
    13621 cagcaggtgg tgctgttcca taaactggaa aatctgcgcg atcgtctgat cgaccagggt
    13681 gatgacgcca tcgctgaagt attgaatctg tggcccgatg ccgatcgtca gcagctgcgt
    13741 actctgatcc gcaacgcgaa gaaagagaaa gaagggaata agccgccgaa atccgcacgc
    13801 cagattttcc agtatctgcg cgaactggcg gagaatgaag ggtaattttc gttgttgatg
    13861 tgagggatgc ggtgcacctg taggcctgat aagacgcatc aagcgtcgca tcaggcacta
    13921 tgctcaaatg ccggatacgg cgtgaacgcc ttatccggcc tacgcacact atatcaggct
    13981 gaggccgaat tactgcgcag cttccgcctt cttcgccaga ccatccagca gtttctgatg
    14041 gatgccacca aaaccgccgt tgctcatcac cagaatatgg tcgccaggct gagcggtttt
    14101 caccaccata tctgccagcg tatccacatc gccactccag tgtgcaggct gaacgcaggc
    14161 ttctgccact tctgccacct gccacggaat atgcgccggt tgcagcagga agacttcatc
    14221 ggcacggcct aatgaaggtg ccagatcgtc tttgcagatc cccattttca tggtattcga
    14281 gcgcggttcc agcacagcaa taatgcgcgc cgtaccgcca actttgccac gcagcgccgc
    14341 cagcgttgcc agaatcgccg ttgggtgatg ggcgaaatca tcatataccg tgacgccatt
    14401 cgcttcacca cgcagttcca gacggcgacg agcgttaata aacgaaccca gcgcattagc
    14461 ggcatctgcc ggcgctacac caacatggcg agccgccgca atcgccatca ggccattgtg
    14521 catattatgt tcgcctacca gcgaccattt cacttcgccc actttttcgc catccagcaa
    14581 gacttcccat tcggaagcat cggtggtcag ctttttcgcc tgccagtgcc cctgctcacc
    14641 caccagctcc tgctcgctcc agcagcccat cgccatggtc tgtttcaggt tgatgtcatt
    14701 ttccggccag ataatacggc cctgccccgg aacgatacgc accagatggt ggaactgttt
    14761 ctggatcgct ttcaggtcgt caaagatatc ggcgtgatcg aactcaaggt tgttgaggat
    14821 cagcgtacgc gggcagtaat ggacaaattt agagcgtttg tcgaagaagg cgcagtcata
    14881 ctcatccgct tcgataacaa agaagtcgct ttcgcccaga cgcgccgaaa cctcaaagtt
    14941 ccccggcaca ccgccgatca caaatcccgg tttgtaaccg cactgttcca gaatccaggt
    15001 tgccattccc gcggtggtgg ttttgccatg tgtaccggca acggccagca cccagcggtc
    15061 acgcagcaca aaatcgtgca gccactgtgg acctgacata taagggatgt ttttttccag
    15121 taccgcttcc acacacggat ttccacgggt catggcgttg ccaataatca ccagatccgg
    15181 ctgcggatcg agctggctag catcgtaacc ctgaatcagt tcaatgcctt gcttctcaag
    15241 taaggtgctc atcggcggat acacattggc gtccgaaccc gttacttcat ggcctaactg
    15301 gcgcgccagc atcgccagac cgcccataaa cgtgccacaa attcctaaaa tatgaatgcg
    15361 catacgtcac tatccttttt taatctgccg ctcattttac gcatatgtcg ggcaagtgag
    15421 aaacgcattt caggataatc cgcaatttgc tggcgcgatt cacctgagcg caacattccg
    15481 attatttgtt aagattgtta cggtcgcttt actccataaa cattgcaggg aaagttttat
    15541 gaaaacgtta ggtgaattta ttgtcgaaaa gcagcacgag ttttctcatg ctaccggtga
    15601 gctcactgct ttgctgtcgg caataaaact gggcgccaag attatccatc gcgatatcaa
    15661 caaagcagga ctggttgata tcctgggtgc cagcggtgct gagaacgtgc agggtgaggt
    15721 tcagcagaaa ctcgacttgt tcgctaatga aaaactgaaa gccgcactga aagcacgcga
    15781 tatcgttgcg ggcattgcct ctgaagaaga agatgagatt gtcgtctttg aaggctgtga
    15841 acacgcaaaa tacgtggtgc tgatggaccc cctggatggc tcgtccaaca tcgatgttaa
    15901 cgtctctgtc ggtaccattt tctccatcta ccgccgcgtt acgcctgttg gcacgcctgt
    15961 aacggaagaa gatttcctcc agcctggtaa caagcaggtt gcggcaggtt acgtggtata
    16021 cggctcctct accatgctgg tttacaccac cggatgcggt gttcacgcct ttacttacga
    16081 tccttcgctg ggcgttttct gcctgtgcca ggaacggatg cgcttcccgg agaaaggcaa
    16141 aacctactcc atcaacgaag gaaactacat taagtttccg aacggggtga agaagtacat
    16201 taaattctgc caggaagaag ataaatccac caaccgcccg tatacctcgc gttatatcgg
    16261 ttcactggtc gcggatttcc accgtaacct gctgaaaggc ggtatttatc tctacccaag
    16321 caccgccagc cacccggacg gcaaactgcg tttgctgtat gagtgcaacc cgatggcatt
    16381 cctggcggaa caagcgggcg gtaaagcgag cgatggcaaa gagcgtattc tggatatcat
    16441 cccggaaacc ctgcaccagc gccgttcatt ctttgtcggc aacgaccata tggttgaaga
    16501 tgtcgaacgc tttatccgtg aattcccgga cgcgtaatct cctgcgcctc tccacgcgga
    16561 gaggcgtttt tatcgctgcg caatgttgac tcttgtcact ggcgaactct gacgattctc
    16621 ccacagcacc gttaatccac gctgtaatgc tataaaaata aacaacaaaa taccgatggc
    16681 gattttcgtc caccaggaac tcagcgtgcc atcaaagttt atgtaagtct gaatcagtcc
    16741 ctgaatcgcc acgccaaaaa gcgtccctaa taccgttcca acgccaccgc tcaaaagcgt
    16801 accgccaatt accactgagg cgatagcatc cagttccaca cctacgcccg ccagcgcata
    16861 tccggcctgg gtataaatcg agaagacaat ccccgccagc gttgccagtc cggtggagag
    16921 catataaatg cgaatagtgg tgctgcgagt ggaaatcccc atcaggtttg ccgacgtcgc
    16981 attgccccca atggcgtaca cctgattacc aaaacgggta cgatgcgcga ggaaaatacc
    17041 gataaccacc accgccagca tcagcagccc catcgcactt aagcgaccgc cgccagggat
    17101 tttccatgca aggcttgaga gcgtgtcata aacaggatgg tttatcggga tcgactcttc
    17161 cgaaacgagg tagctgacgc cgcgcaaaaa gaacatcccc gccagagtaa tgataaatgc
    17221 cgggatcttc agggcgtcga tcaaaagccc cataaatgcg ccaaaggcac agcccattac
    17281 cagcaccagc ggaaatgcca gcaacggcga gaggccgaag tcgccaatca cttttgccag
    17341 aaacacgccg gtaaaggcga tcaccgaacc gacggagaga tcgatcccac ctgagaggat
    17401 cacaaaggtc atgccaacgg caatgatccc aagaaaggcg ttatcggtca ggatattgca
    17461 gatcactctt gtggaagcaa aaccaggaaa ctgcgtcagg cagtagagat agcccagcac
    17521 aaagacgccg atggtgatca taagcggcaa gttacgtttt atcacggcta cgcactcctt
    17581 taatcagact gataaagcgt tgcgactgaa caatcagcac gcaaagcacc accaccgctt
    17641 tcaccacctg gttcatctcc ggcggaaagc ccgaaagcaa aattccggtg ttcatcccct
    17701 gaataatcag cgcccccacg actgagagca gcaggttaaa gcgcccgccc atcagcgatc
    17761 cgccgccaat caccaccgcg agaatggcgt ccagctccag ccataatccg gcgttgttgg
    17821 catcggcacc gcgaatatcc gccgccacga taatgcccgc aatcgccgca catagcccgc
    17881 tcaacacgta ggtgagcatg acgatgattc gcgtgtttac cccggcattt tttgccgccc
    17941 gaatgttgat accaacggct tcgataaaca tccccagcgc cgttttgcgg gtcaacagcc
    18001 agaacaggag aagcgtcagc accgcgataa tgaccggcgt tggcaggaac aacagcgatc
    18061 cgctgccaaa ccatgagaga tccggcgagt taaacgtgac gatctggccg gaggtgatca
    18121 gttgcgccac gccgcgcccg gcgaccatca ggattagggt ggcgacaaat ggctgaattt
    18181 tgaggatcgc taccagtatg ccgttccaca atcccgccag gatgccggtg cccagggcgc
    18241 ttaacaaaac aatcggcagg ctgactcccg cgaccgtcat cgcagccgtt gtggctccgg
    18301 cgatagccat caccgcccct acggagagat caatcccacc ggtggcgatc accagcgtca
    18361 taccaatcgc cagtaacgca acgggggctg cacggttaag aatgtctatg gggctaccga
    18421 acaaacgccc atcctgaagc actacctgcc agaaatgcgg ggctaccagg ctgtcgacca
    18481 gcagcaccag caatagtgct accagctgcg gcatccccgt cggccagcga aagcgccttt
    18541 ttggcgtagt ggtgtccgga agagattgag gcatcacact gttctcctta cgccgcaatg
    18601 gcgttcatga tcgccggaac ggaaagctct gccagcggga tctccgccac ctgtttgcga
    18661 tcgcgcatga tgatcacccg gtcggcatag cccaccagct cttccagttc ggaggagatc
    18721 accagcagcg ccagaccatc ggcgcatagc gtttcaatca ggcggatgat ctcggcgtgg
    18781 gcaccaacat caatgccacg ggttggctca tcgaggatca gaaattgcgg tcgggtcagt
    18841 agccaacgtg aaagcaacac tttttgctga ttgccgccgg agagaaattc aatcggttgt
    18901 tcagttgaag gtgtgcgaat gccaagctgg cggataaagc gttcggcaat ctcttgctgc
    18961 tctttgcggg aaatgggtcg tagccagccg cgctgggcct ggagagcgag gatgatattt
    19021 tcccgcaccg aggcggcagc gatgatgcca tcggttttcc tgttttccgg gcagaagccg
    19081 atgcccagta ccgaagcctg atgtggcgat cgcaggtttt gcggtttgcc tttgatcaac
    19141 gccgtgccgc tgtcagcagg tttgataccg aagatcactt cggcggtttc ggtacgtccg
    19201 gatcccagca agcctgccag gccgacgatc tcgccggggc gcacttcgag atcaaacggt
    19261 gctatcgttc cttttttgcc gtaatttttg aacgcggcaa cgggtttgtc gctcaacaat
    19321 gttcgcccgg cacgctgtag cgcgtgggta tccagctcgc gccccagcat catttttacc
    19381 agttcgatct gcggtagctc gcgcgtttcc cgacagccta cgaaactgcc gttgcgtaaa
    19441 acggtgatcc gatcgctgac ctgatagacc tgatcgagaa agtgagtgac aaagatcagg
    19501 ctgacgccgc gatcgcgcaa ctgacgcatc aggccaaaca gtaattctac ttcctgagtg
    19561 tcgagactgg cggtgggttc atcgaggatc agcactttgg cagagagatc gatagcccgg
    19621 caaatggcga cgatttgctg catcgcgact gaaaagcggt taagcggttc acgcacgtcg
    19681 agggagaaac cgtaagatgt catcagttcg gtggcacgct tttccatctc tttgcggcgt
    19741 agaaagccga aacgtttggg ttcgcgacct atgaataaat tatcagcgac cgacatattg
    19801 ggtagcaggt tgacttcctg ataaacggtt ccgatgccga gttgttgtgc atgggcggta
    19861 tttttcggtg agatagcctg gccttccagc cagatggtgc cgcgatcggc gtggtataca
    19921 ccggttaatg ctttgattag cgtcgatttt cccgccccgt tttcaccgag cagcgccatg
    19981 atttcgccac ggcgcaggct gaaatcaacg ttgtctaacg ctttgacgcc ggggaaaaat
    20041 ttacttaatc cttcggtgcg gaggatttcc tggtgttggt cggtcgtcat ggtttccccc
    20101 ttagccacgc tctaaaagtc tgggaataga tcgtgcctga tgttaagagc tgcgccgttt
    20161 gtccccctca ccctaaccct ctccccgaag gggcgagggg acagattgtg cacggtgtta
    20221 agtccggtgt tgcaccactg ccggatgcgg cgtgaacgcc ttatccggcc tacacagagc
    20281 accgaactcg taggcatgat aagacgcggt aagcgtcgca tcaggcttca gcgcaccacc
    20341 gccggatgcg gcgcgagcgc cttatccggc ctacgaatcg gcatcaggca tagcaaccct
    20401 caatacccca tatttttctt cttctctaac tcttcttttg cggtatcagg caggtaaagg
    20461 gtggatttgg ttaacgtcag cttttcaggc atggtgccgt cttttttgta tttctccagc
    20521 gcgtcaaaag cgggtcccgc catattcggc gtcagttcaa cactggcgtt cgcttcgcca
    20581 tcaatcatcg ctttgtagat gtccggtaca ccgtcaatgg aacccgtgag gatatctttg
    20641 cccggtttca ggcccgcttc tttaattgcc tgaattgcgc caatcaccat gtcatcgtta
    20701 tgggcgtaaa ccatgcagat gtttttgccg ttgttttccg ctttgataaa gctttccatg
    20761 acttctttgc ctttactgcg ggtaaagtca cctgactgcg agcggataat tttgatattt
    20821 ggcgcattct taatggcttc ggcaaagcct ttcttgcggt caatggcgac gctggcccca
    20881 acggtgccct gcagctccac cacgttgcat ggtttgccat tcacttcttt taccagccag
    20941 tcaccgatca acttaccttc gaggatgttg tcggcagtaa cggtggtcat atagagagat
    21001 ttgtctttca catcgatgga acggtcgagc aagaagaccg ggatttcggc atctttcgcc
    21061 tcttttaata ccggttccca acctgtcgcg accaccggag cgataaagat cgcatccacc
    21121 ccttgcgcga cgaaggaacg taccgcttta atctggtttt cctgcttttg ctgaccatcg
    21181 gcaattttca gcgtaattcc gcgtttttcg gcctcacttt tcgccacatt ggtttctgca
    21241 gcgcgccagc cggattccga tccgacctgc gaaaatccta cggttaatgg agcggccaac
    21301 gccatagacg acatggctgc cgagactgca gagactacaa gtaagcgttt ccacataggg
    21361 ttgtcctcgt cggggtaatt attattggtg aaaagatgtt cgcggaaaaa ctatagacaa
    21421 ttcgttatgt aacggattgc gttacatcac acttcataaa agtaaataaa acgttaatca
    21481 caagtttgta atcgctttca tctcactatg aaaaatgcgg ctacggttat ggattttcct
    21541 gctctgtata ccgtcttaaa actggcgaaa aaggaaaatg aagacgaaaa caagcgaaga
    21601 cattcggcgc gagttggcta taatactcgg cacttgtttg ccacatattt ttaaaggaaa
    21661 cagacatgag cttactcaac gtccctgcgg gtaaagatct gccggaagac atctacgttg
    21721 ttattgagat cccggctaac gcagatccga tcaaatacga aatcgacaaa gagagcggcg
    21781 cactgttcgt tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac tacggctata
    21841 tcaaccacac tctgtctctg gacggtgacc cggttgacgt actggtcccg actccgtacc
    21901 cgctgcagcc gggttctgtg atccgttgcc gtccggttgg cgttctgaaa atgaccgacg
    21961 aagccggtga agatgcgaaa ctgattgctg ttccgcacac caagctgagc aaagaatacg
    22021 atcacattaa agacgttaac gatctgccag agctgctgaa agcgcagatc gctcacttct
    22081 tcgagcacta caaagacctc gaaaaaggca agtgggtgaa agttgaaggt tgggaaaacg
    22141 cagaagccgc taaagctgaa atcgttgcct ccttcgagcg cgcaaagaat aaataagttc
    22201 ttatagcgca ataaccctga acaccgggct tcggttagta agggtttttt tatgcccgcg
    22261 ataaataaac tctctattcc actttcatta ttctcagcgc ttgcaaggct tgaacggtaa
    22321 gaacaagcaa acccgaccac cattttgctg ttcatagcca cttgctggaa gttagccgac
    22381 ctcactcata ctcaccgttc aaagcgtgcc cccgttttac ccctgcacat ttgtgaacgc
    22441 cagcagagtt ttttatgcga ttgcagatat ggaaagcggc tcggagagaa aagcaaaaga
    22501 tggaaaagtt acaaaaaaaa atcgcgagat acatcgcata aaacaacgcc acccgaaggt
    22561 ggcgtgcttt tattattact tatccctgtc taaccagtcg ccgctttcaa ttatctttaa
    22621 tccatcgacg ggtcgttgat aaacgtacat ccatgcactc ccgtacggcg tctgaatcaa
    22681 ctggcgcgcg tattcacctc ccctggtgcg caaggcatca agttcggcca gcgtggcgtt
    22741 gtcaatacga taaacttcac cgtgtactgt tccgttcccc ggaactgcgc ctggatagtg
    22801 gcccaggcta tacaactggt agttatcgat actgaaatcg cccagtaact gggcattggt
    22861 catccagtga ctgttgcctt gtttgtggcg taaactgccg tagacaaata ttcgcattgc
    22921 taaaactcga actgatagag caaatccagt gcctggtcta caccagacac ggcttccaga
    22981 tatagcttag gcatcaggcg ataacgtaac gtgagtgttg ctatagagtc aaatataccc
    23041 acgccgtatt tcacttgcag acctggcaat acatagccgc tgaccactac ctgggaggag
    23101 tcgcctactc cctgggtgtc gagcgctaaa ttgcttacgc caaacgtctc gccgatttta
    23161 cccacaatct ggccactttg cgcaaccccc agaccaatca gcatcgaggt cattgccgca
    23221 ctgtcgctct gatcgctctc cagcccttgt ccacgtagca aataagacaa tgcagcttgt
    23281 tgcgacatcg ccgggtcaga gaagatctcc gctttcggtt cgtccgccag accagtgacg
    23341 cgaactccgg cgattacgtc gtcttctgta gcatccgggt tacgaatagc ttcaatatta
    23401 agatacggtt gatccggcgg accagagaac agtaactcac ccttacgcac aatcagatcc
    23461 tgaccatagg catggaagcg cccttcaggg atgttgatct gcccgttcag gcccagccct
    23521 tgtttgtcct gtacgacgtt aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca
    23581 atgcgcacat tgttgccaac gtggacaatc aggttactgt taatcggaat cgacgccgtt
    23641 ttcggctctt ccggttgcag gttatcgtta agcatcacca catcgctgga gacgcctact
    23701 gcgctttccg gcagatcgtg caccacgatg cgcgcccacg ggacatccac gcgaccatcg
    23761 agggtaaaca ggtttggtgt agcctcgaat acaacatctg gcgatacatc cattcgtacc
    23821 atcggcggca cggtgatccg cactttactg cctttcgccg ttactcgtgc ccgccagttt
    23881 tcaatttggc tccagtcggc atcaccgttc aggtagattt caccctgctg ggtccgtact
    23941 gtaccggcaa gcgtcgagcg cataccgtta aagttgaccg caagctggct cggctgcata
    24001 tcaaacggca taaagttgcc gtcgatatcc acacccgtaa cctgaagctg accaaacaac
    24061 tgcgggcttt gcacatcacc acccagacgc aagttggcac tcaccatccc cgctgctttt
    24121 tccccacggg taaagatggg gtttatcatc gcaaggttga agttacggat attgacgttg
    24181 ccaccaagat tacggcggcc ttgcggatcg gtcacctgca cctgtccatc aaactggccg
    24241 ttattggtca ggcggatggt ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc
    24301 agattcagtg tctgaaacgc caccggcagc gccgcatcgt tgacggtttg cgttacctgc
    24361 acgttacgcc ccgaaagggt gatactgccc tgcggcagcc cctctttcgt ggtgtcccag
    24421 gcaacatccg ctttacccgt gaagataccg ctggcctgag tggtttgcgg cataaacggt
    24481 ttcagcatgg cgaggtcgaa gcggttgaga ttcaccaccg cacgcccttc ggcccccgca
    24541 tcgatagttt gcggcacgca cagctccgca ttcgggttaa gccaacagtg tggcccgatg
    24601 ctgatttttt gctccttatt gcggtaatcc agcgcaatat cgcgggtcag cgaccacggg
    24661 ccgaccggcg tctggaagcg ggtattgcta agagttccct tccagcgttc ttctttgcga
    24721 tcaaaacttc ctgccagatt aagctgcccg gagacaggct cgccctgaat ccgcaactgt
    24781 agctcgtgct gcttttcgct gcctttggca ttcagggtga cgaggttgat atttacatcc
    24841 ggctgcgaaa tttgctcaac gcgtacgtcg agtttcccgg cgatctgatc ggtggattta
    24901 atgtcgcctt ccacgcgaac ctgcgccacg gaaagttcct gccagcgcag gccgcgcgcg
    24961 gtgatatctg ccagtagttg tggcgcttcc accgtgccgc gtactttcac cagccctttc
    25021 gctgtaccgc caagccccgg cagcgcgtta tccagccccg gcgcgttgat ggtggcatca
    25081 agattgagat cttttacccc cagctcgcct ttcacttcgg cactgtttgg ccccagttcc
    25141 agatgaagcc ctgggatcat ccactgcata taactgttgc ctttcagcgt gccgtcaacg
    25201 ttcactttgt tctgtttgac gttaccggtc agcttcagtt ctggcacctc catctgccag
    25261 gtgccgccgt acaggctacc gcgggtttta atcaagccat tgagtttcga cggccactcc
    25321 gggaactctt tggcggtgtt aatgccatta agcgttagct caccgcgcca actaatggcc
    25381 tgttgccagt cgagcaacgc cttgagttca gttttgcctt ccagcgccgc aacggtgagt
    25441 ttgtcgagat tgacctgctg ttcattacct ttcgcgtcga gggtaatagt cgcgggcggg
    25501 atctcctgtc ccttcactgc cgtacgcata gagagcgtgt aatcggtcat tttgccggta
    25561 agtttcagtt tcagatcatc cgcctgatac tgcttctcac cagtgaacgg ccagtaaagc
    25621 tgtttgctgt tcacttccac gttgagcggc aatccagctt ccgccagtcg cgtctgggcg
    25681 cgtaaatcca tatccaccgg accggaaagg ttaacgccaa tctccagctg ttcgcgcagc
    25741 gcgccgccca ctttcagctt cactttttca cctttcaacg gctccacgtt tagtgtgctg
    25801 ttgagggtga tatccaccgg ccagttgtct gacagctgtg ccgtgccgct ggcgttgacg
    25861 atcccttgat tggaatcgat atccagggcg tccagtttag tattaccgtc gatgctgctt
    25921 actttcagca gcatggtgtt cacggtgatg tccgtgtcgc ccgtcacgcg cagctgctcg
    25981 cccttaaact cttcaatgtt caggttaagc ggcaaatgca cgtcggtcat ttccggcaat
    26041 accggacgag aaaagagatc tttcaacgtt tcgccgagcg gcttttcatc cggctgcgga
    26101 ttttcaattt tcggttcgac cacttcttcc tgcgccactt ccgccacttt cggcagggca
    26161 atcaacaggc ctttcagcga cgtcggtttc agggtcaggg ttttctcctg ccagttcagg
    26221 ccggaggtga agtccatcac cgataccgcg gtgtcatcaa tcttgatgtt gacgttatcc
    26281 agcgccaccc gtgtcagggt gatgggatac ggcgtggaga gatccagcgg accgctatct
    26341 tcctcttctt caacctgttc agaagggggc atttttttac tgtcgatgtt gacctgaatg
    26401 tctttcagcg ccaggtcatt aatacaaacg ctactgttcc acaggcactc aagaccgacc
    26461 gccagatgca gattacccgc ttttaccgcc acgcctggct gctcataacg aacgtcagac
    26521 aaggtgagat cgcgccagcc gccggtgacc ttgccaatat ccagtcctgg cacccagcga
    26581 tccgccgctt taaatacaag atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat
    26641 cccagcaaca gtaagataac gataaccacg ccgaggctga tttttttcca taaactcata
    26701 attctggccc cagaccgatg taaaactgta acccgtgctc gtctttatcc gcgaccggta
    26761 cggcaaaatc gagtttaatt ggcccgaccg gcgattccca gcgtacgccg accccggtac
    26821 cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc gccactatcg acaaacaccg
    26881 cgccccacca ttttccggtc acgttgtact ggtattccag cgatccggtt atcagcttcg
    26941 aggccccttt caggtcaccg ttggcgtatt tcggggcgat agatttgtat ttgtagccgc
    27001 gaatactgcg atcgcccccg gcgaagaaac gcagatccgg cggtactttg tcgaaatcac
    27061 ccgtttcaat ccagcccagc gtgccgcgtg tcacaaaacg atggcgatcg tacagtgtgc
    27121 ggatccagac gttctgcgcc tggaaaacgg agaaatcgac atctgagccc caggccgtgt
    27181 tggagtagtc gatagagtag cgttgcgagt cgccccaggt tggcatcagg ccaccacgag
    27241 aacgcgtgcg gctaatcatc accccaggat aaaacagcat cgtggtgttg gtaatttcac
    27301 cctgggtaaa gtggtcgagg ctccagcgca ggttaatggc acgctgccag ccgctggaga
    27361 gatcccagta gcgagaagcc accagcgtag tggagtcaga ttcggtatcg ttcaggtcag
    27421 tgcgcttaaa accgccctgc accaaataat attgttccag tggattcttc agcagcggca
    27481 ttttatagct gaagtcgagg gtctgttccg gcgcggaaat actggtactg gtggtcagac
    27541 tgtgaccata agagttcatc cacggctttt tccacgtcgc tttcacgcgc ggtcccacgt
    27601 ccgtagagta accgaccccg gtttcgatgg tgttttcagt tcgcggcgaa accacgcccg
    27661 tcaagggtaa tactttcgtt tcgcgcgctt tatcaaattg tggagccacc accaccgagt
    27721 taaaccagcc ggtagcagaa agtcggcggt tcagttccgc cagatctttc gattcgtact
    27781 catcgccctc tttaaacggc accagatttt gcaggtattc atcgcggatt tgtgatcctt
    27841 caaaggtcac atgcccaaag cggtaacgtt cgccactgtt ataatcaata tcccagaagg
    27901 ctttatgcag gccgagcgca atgcccagct gcgctttggt aaattcgcta tcgaaataac
    27961 ctttacgcaa cgcaatgctg gttaaggact ttttgaaatt ttcataatcg ccctggttca
    28021 gcaccgtgcc aatagccggg cgagtatcga gcaatttcaa atagtcttta tcggtccgcg
    28081 cgccgccgcg caataccaca tcggtgccgc caattaacac cggcacacct ggcgtgactt
    28141 tggcgatcaa tacctgccgc cctttctttg gcggtggacg gagatcaaat tcaatggtcg
    28201 gctggtaata acccagcgct ttcagacctt cgcggatggc atcatcgacg cgtgcgcgaa
    28261 agcgacggtc tggcgtcact tcatcacttt caatcgtaga aagctgcgca cgaacgttct
    28321 tttccagctg tcccgataac ccctcgacct gtagacggac gttcgcggcg acggcagatc
    28381 cgcttaagca gagtaagctt acacagcata actgtcggat atagcgcaca ttttctcctg
    28441 aatatccttt tttcctgccc ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata
    28501 tggattaaaa agcagactaa accccaaata tttcttatgt tttactttag acctattcac
    28561 ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt aaccccaatg accgattttc
    28621 gggagagcga caccatgagt ttatttgata aaaagcatct ggtttccccc gccgatgccc
    28681 tgcctggacg taacaccccg atgcccgtag ccacgctgca tgcggtcaac ggtcactcaa
    28741 tgaccaatgt acctgacgga atggagattg ccatttttgc gatgggttgt ttctggggtg
    28801 tggagcgtct gttctggcag ttacccggcg tttacagcac cgccgcaggc tataccggcg
    28861 gctatacgcc aaatccgact tatcgggaag tgtgctccgg tgatacgggt catgccgaag
    28921 cggtacgcat tgtttacgat ccttccgtca tcagctatga gcagttgcta caggtatttt
    28981 gggagaatca cgatcccgcc cagggaatgc gtcagggcaa tgaccacggc acgcagtatc
    29041 gttcagcgat ttatccgctg accccagaac aggatgccgc agctcgcgcc agtctggaac
    29101 gttttcaggc ggcgatgctt gccgccgatg atgatcgtca catcaccacg gaaatcgcta
    29161 acgccacacc gttttattat gccgaagatg accaccagca atatctgcat aaaaacccgt
    29221 atggttactg tggaattggc ggaattggcg tctgtctgcc gccggaagca tagcgttacg
    29281 ggtataaatg tagattgttg ataaagtgcg ttttgtttat gccggatgcg gcgtaaacgc
    29341 cttgccaggc ctacaaaatc gtccaaattc aatatattgc aaggactgcg taggcctgat
    29401 cggcatagcg catcaggcag ttttgcattt ttcagctgtc tgaattttaa ccctctggcg
    29461 gctttacagt accttacgct atactagcca ctgaaaatgc cggttcactt tcttcgaatc
    29521 ggctttcaat gtgtatttca cacaaattaa tcaacttccc cttccgagga tctggcctga
    29581 aaggtcggat aagatatgtt aaacagtatt ttagtcatac tctgcttgat cgctgtaagt
    29641 gcgttcttct cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa acttaaactg
    29701 ctggctgatg aaggcaatat aaatgcccaa cgcgttctga atatgcagga aaatcccggc
    29761 atgttcttta ccgtggtcca aatcggtctg aacgcagtgg caattctcgg cggtatcgtc
    29821 ggtgatgcgg cattttctcc agcttttcac agcctgttct cccgctatat gtcggcagaa
    29881 ctctctgagc aactgagctt tattctctct ttctcgttag tgactggcat gtttatcctg
    29941 tttgcggatt taaccccgaa acgcatcggt atgattgcgc cagaagcggt ggctttgcgt
    30001 atcatcaacc cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac
    30061 ggcctggcga acataatctt ccgtattttc aaactgccaa tggtacgtaa agatgacatc
    30121 acttctgatg acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa
    30181 caggaacacg agctgattga aaacgtcttt gagctggaat cccgtaccgt tccgtcttca
    30241 atgacaccgc gtgaaaacgt gatttggttt gatctccacg aagatgagca aagtctgaaa
    30301 aataaggtgg cggaacatcc gcactctaag ttcctcgtct gtaatgaaga tattgaccac
    30361 atcatcggct atgtcgattc taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg
    30421 gcactgaaca gcggcgtaca aattcgcaac acgctgattg tgccggatac gttaaccctt
    30481 tcagaggcgt tggaaagttt taaaaccgca ggtgaagact tcgcggtgat catgaacgag
    30541 tacgcgctgg tggtggggat catcaccctc aacgacgtga tgaccacgct gatgggcgat
    30601 ctggtcggtc aggggctgga agagcagatt gtcgcccgtg atgagaactc atggctgatt
    30661 gacggcggca ccccgattga cgacgtcatg cgcgtgctgg atattgacga attcccgcag
    30721 tcgggcaact acgaaaccat cggcggcttt atgatgttta tgttgcgtaa gatcccgaaa
    30781 cgcaccgatt cggtgaaatt cgccggctac aaatttgaag tggtggatat cgataactac
    30841 cgcatcgacc agctgctggt gacccggatc gacagcaagg ccacagccct ttcgccaaaa
    30901 ctgcctgacg ctaaagataa agaagaaagc gtcgcgtaac ccccataaac atcaacggct
    30961 cctgaatcag gagccgtctt attactgcat agcactttgg ttaagccatc tctgtttgca
    31021 gacgcataac ctgacggtta acttcggaca tcactgacaa atgcagctta tccttcactt
    31081 ttgggataag aatcttaccc ttatcaaatt caaaagcgcc aacgtccttg atgtataacc
    31141 gtccacggaa caggatcttt acgtacttcg ccacctgaag tgggttgtac cgttggaaaa
    31201 ttttcattgt tatctcctgc tgagtattac gcctgtgcgg tgccacaatc ggcccaacta
    31261 ttatgaggcg caaattttaa tgcttagtga ctatagacta tccgggcaat gtttccaccg
    31321 cgtataactt tttttaccta aaggttacaa ttattcagaa ttatcttttt accgaagcgc
    31381 ggtcttcagt ataagcattc atttttcata tgatttgtgc gcttgaccgc aaactggcat
    31441 cacacttgcg ggaaattcga taaatagcac atatgattaa aactcagacc caagtggtcg
    31501 gatcacctgc atatcataag aaggaaacac catgacccta cgcaagattc tggcactcac
    31561 ctgcctgctg ttgccgatga tggcttccgc acatcagttc gaaaccggtc agcgagtgcc
    31621 gccgattggc atcaccgatc gtggcgagtt ggtgcttgat aaagatcagt ttagctacaa
    31681 aacctggaac agcgcgcagt tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg
    31741 cacctctgca aaagagaaaa acgcgacgct gattgaagcg attaaatcag cgaagttacc
    31801 gcacgatcgt taccagacca ccaccattgt taacaccgac gacgcaattc cgggttccgg
    31861 catgtttgtg cgcagcagtc tggagagtaa taaaaagctt tatccctggt cgcagtttat
    31921 tgttgatagc aatggcgtcg cacgcggtgc ctggcagctg gatgaaaaaa gttccgctgt
    31981 agtggtgctg gataaagacg gtcgcgtgca atgggccaaa gacggggcgc ttactcagga
    32041 agaggtgcag caagtgatgg acctgctgca taaattaatt aataaataga cactctgaac
    32101 ccaggattca ggaacgactc gcgcggagtg taatccagcg gtttaccctg ccagtcgtga
    32161 acgtgcgctc cggcagctgc ggccacagca tgtccagcgg cggtgtccca aatattcgtt
    32221 ggtccgaagc gcgggtacag ctgcgcctgt ccttccgcca ccaggcagaa tttcagcgaa
    32281 gagccgatgg acgtggtctg atgttcgcca agctgttgca gatactcttt cagctccgca
    32341 tctgcatggg aacggctgat aaccaccagc ggcgggcgcg catcgcggac ctgaatctgc
    32401 ttgcgcacac cgcactcttc tttccaggcc ttgccttctg ccgcgctgta cattacgttc
    32461 attaccggcg catacaccac acctaaaatc ggtttgccat ggtcaatgag cgcaatgtta
    32521 acggtgaatt cgccattgcg tttaataaac tctttagtac catccagcgg gtctaccagc
    32581 cagtaacgct gccagtgctg acggacttcc caaccgggag gatcttcttc agaaaggacc
    32641 ggaatatccg gtgtcagcgt acgtaaaccg tccatgataa cggtgtgtgc ggcaatatcc
    32701 gctgccgtta ccggagaatt gtccgctttg ctgacgacgt ccatcggttt cgtcccgtcg
    32761 tagacctgca taatggcatc gcctgcattc cgtgcaagct ggcatacttg atctaacatt
    32821 tctccacctc gtctctgtga gcggtgttaa cttattgttt tacttatacc ctatcgttaa
    32881 tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg taaaattgtg gcattcttca
    32941 ctgttctata agtaagacgt ttattctccc ttttctttcg tattcccgat gataaaagga
    33001 tgtccctgat gattaagttt agcgcaacgc tcctggccac gctgattgcc gccagtgtga
    33061 atgcagcgac ggtcgatctg cgtatcatgg aaaccactga tctgcatagc aacatgatgg
    33121 atttcgatta ttacaaagac accgccacgg aaaaattcgg actggtacgt acggcaagcc
    33181 tgattaacga tgcccgcaat gaagtgaaaa acagcgtact ggtcgataac ggcgatttga
    33241 ttcaggggag tccgctggca gattacatgt cggcgaaagg attaaaagca ggtgatattc
    33301 acccagtgta taaggcatta aatacgctgg actataccgt cggcacgctt ggcaaccatg
    33361 agtttaacta cggtctggat tacctgaaaa atgcgctggc gggagcgaaa ttcccttatg
    33421 taaatgccaa cgtcattgac gccagaacca aacagccaat gtttacaccg tatttaatta
    33481 aagacaccga agtggtcgat aaagacggaa aaaaacagac gctgaagatt ggctatattg
    33541 gcgtcgtacc gccgcaaatc atgggctggg ataaagctaa tttatccggg aaagtgacgg
    33601 tgaatgatat taccgaaacc gtgcgcaaat acgtgcctga aatgcgcgag aaaggtgccg
    33661 atgtcgttgt cgttctggca cattccgggc tgtctgccga tccgtataaa gtgatggcgg
    33721 aaaactcagt ttattacctc agtgaaattc cgggcgttaa cgccattatg tttggtcatg
    33781 ctcacgccgt tttcccgggt aaagattttg ctgatatcga aggggctgat atcaccaaag
    33841 gcacgctgaa tggtgttccg gcggtaatgc cgggcatgtg gggcgatcat cttggggtgg
    33901 tcgacttaca actcagtaat gacagcggta aatggcaggt gacgcaggcg aaagcggaag
    33961 ctcggccgat ttacgacatc gctaataaaa aatccctcgc ggcggaagac agcaagctgg
    34021 tagaaacact caaagccgat cacgatgcca cacgccagtt cgtcagcaag ccaatcggta
    34081 aatctgccga caatatgtat agctatctgg cgctggtgca ggacgatccg accgtgcaag
    34141 tagtgaacaa cgcgcaaaaa gcgtatgtcg aacattacat tcagggcgat ccggatctgg
    34201 caaaactgcc ggtgctttca gctgccgcac cgtttaaagt tggtggtcgc aaaaatgacc
    34261 cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt ccgtaatgcc gccgatcttt
    34321 atctctaccc caatacgctg attgtggtga aagccagcgg taaagaggtg aaagagtggc
    34381 tggagtgctc cgccggacag tttaaccaga ttgatcctaa cagcacgaaa ccgcaatcac
    34441 tcatcaactg ggatggtttc cgcacctata actttgatgt gattgatggt gtgaattatc
    34501 agattgatgt tacccagcct gcccgttatg acggcaagtg ccagatgatt aatgccaatg
    34561 cggaaaggat taagaacctg acctttaatg gcaagccgat tgatccgaac gccatgttcc
    34621 tcgttgccac caataactat cgcgcttacg gcggcaaatt tgccggtacg ggcgacagcc
    34681 atatcgcttt tgcttcaccg gatgagaacc gctcggtgct ggcagcttgg attgctgatg
    34741 agtcgaaacg tgcgggggaa attcacccgg cggcagataa caactggcgt ttagcaccga
    34801 tagctggcga taagaaactg gatatccgtt tcgaaacttc tccgtcagat aaagccgcag
    34861 cgtttattaa agagaaaggg caatatccga tgaataaagt cgcgaccgat gatatcgggt
    34921 ttgcgattta tcaggtggat ttgagtaagt aaaacacttc tttttcggcc tataaatcat
    34981 caaccgcatc cggcatttat tggcggatgc gatgctggcg catcttatcc gccctacaag
    35041 ccatgcaccg tagaccagat aagctcagcg catccggcag ttatgccgca cgttcatccc
    35101 gcaccgccaa cacctcgggc aaattcaact caatccagtc cgccagtgca gcaacctttt
    35161 cgctcacctg ctcgcccagc ggcgtgaggc tgtattccac atgcggcggc accaccggat
    35221 acgcgatacg gttaagaaaa ccatcctgtt ctaacgcctg taacgactgc gcaagcatct
    35281 tttcactcac cccaccgatt ttgcgccgca ggtcgctaaa gcgatgagta ccttcgcgta
    35341 gcgccaccag aatcaatacc ccccaacggc tggtgacgtg tttcaacacc tcgcgcgacg
    35401 ggcactgttc cgcaaagaga ttaccctctt tcagttgttg cgacaggcta acctgactca
    35461 tttcatactt acctttttgt acgtacttac taaaagtaag tttaggtgtt agcgtattta
    35521 aacacaagac aaaacgatgg agacttccca tgatcgctat tactggtgcc actggccaac
    35581 ttggtcacta tgttattgaa tccttgatga aaacggttcc tgccagccaa atagtggcta
    35641 tcgttcgtaa tccggcaaaa gcccaggccc tggcagcaca aggcattacc gtgcgtcagg
    35701 ctgactacgg cgatgaagcc gcactgacat ctgcacttca gggagtggaa aaactactgc
    35761 tgatctcttc cagcgaagtg ggtcaacgtg ccccgcagca tcgtaatgtt attaatgccg
    35821 caaaggcggc tggcgtgaaa tttatcgctt ataccagcct gctacatgca gatacctccc
    35881 cgctcggcct cgccgatgag cacatcgaga cggagaaaat gctggctgat tctggcatcg
    35941 tttacaccct gctgcgcaac ggctggtaca ccgaaaacta cctcgccagc gccccggcag
    36001 cactggaaca cggcgtattt atcggtgcgg cgggcgatgg caaaatcgcc tcagcaacgc
    36061 gggcagatta tgcggcagct gcggcacgcg tgattagcga agccggtcac gaaggcaagg
    36121 tttacgaact ggcgggcgat agtgcctgga cgttgacaca gttagcggca gagctgacca
    36181 aacagagcgg aaaatccgtt acctatcaaa atctgagcga agccgatttc gccgcggcac
    36241 tgaaaagcgt cggactgccc gacggactgg cggatatgct ggcggattct gacgttggcg
    36301 catcgaaagg tggtctgttt gataacagca aaacgcttag caaattgatt ggccgcccaa
    36361 cgacaacgtt agccgaaagc gtaagccatc tttttaatgt taataactag ttaattaaag
    36421 tggcatcctc ccgcatcctc tctgataatg acgggatgcc gggagcaatc atgtctgctt
    36481 cctgaacttt cttccgacag accaatggat gccagtaatg attagcggcg tgctgtacgc
    36541 cctgttagca gggttgatgt gggggcttat ttttgtcggg ccgttgatcg taccggaata
    36601 cccggcgatg ttgcagtcga tggggcgtta tctggcgtta gggttaattg cgctgcccat
    36661 tgcctggctg ggacgcgtgc gtctgcgtca gttggcgcgt cgggactggc ttaccgcctt
    36721 gatgctcact atgatgggca acctcattta ttacttctgc cttgccagtg ccattcaacg
    36781 tactggcgcg cctgtttcca cgatgattat cggcaccctg ccggtggtca ttcctgtctt
    36841 tgccaatctg ctttatagcc agcgcgacgg caaactcgcg tggggaaaac tcgccccggc
    36901 actgatttgt attggcatcg gcctggcgag tgtgaatatt gctgagttaa accacggact
    36961 ccccgatttt gactgggcac gttatacctc tggcatcgtg ctagcgttag tttccgtggt
    37021 ctgctgggca tggtatgccc tgcgcaacgc ccgctggctg cgggaaaatc ccgacaaaca
    37081 tccgatgatg tgggcgacgg cgcaggcgct ggtcacgctg ccggtttctc tcatcggcta
    37141 tctcgtcgcc tgttactggc tgaatataca aacgccggac ttctccttac cttttggccc
    37201 ccgtccgctg gtgtttatta gtctgatggt tgcgatagcc gtgctttgct catgggttgg
    37261 cgcactctgc tggaacgtcg ccagccagcg attaccgaca gtgattctcg ggccgctgat
    37321 tgttttcgaa acgctggcag gtttgctgta caccttttta ctccgccagc aaatgccgcc
    37381 gctaatgacg ctgagcggta tcgcgctgtt agtgattggc gtggtcattg cggtcagagc
    37441 aaaaccagaa aaacctttaa ctgaatctgt ctcagaaagt tgacacgctg gcagtgagtt
    37501 aaataagcct ctgctacgta agggttatag cttttgcctt aaagatgcat ttaaaataca
    37561 tcttatctta ttaagaatga ggtatcagct atggcttatc gcgaccaacc tttaggtgaa
    37621 ctggcgctct ctattcctcg cgcttcagct ctgtttcgta aatatgatat ggattactgc
    37681 tgtggcggta agcagacgct gtcgcgcgcg gcggcacgta aagaactgga tgttgaggtc
    37741 attgaagctg aactggcaaa gcttgctgaa caaccgattg agaaagactg gcgtagcgcc
    37801 ccgctggcag aaatcattga ccatatcatc gtgcgctacc acgatcgtca ccgcgagcag
    37861 ttgccggagc tgatcctgca agcgactaaa gtcgagcgcg ttcacgccga caaaccgagc
    37921 gtgccaaaag ggctgacaaa atacctgact atgctgcatg aagagctttc cagccacatg
    37981 atgaaagaag agcagatcct cttcccgatg atcaaacaag gcatgggcag ccaggcaatg
    38041 gggccaatca gcgtaatgga aagcgagcac gatgaagcgg gtgaactgct ggaagtaatc
    38101 aaacacacta ccaataacgt cacaccgccg ccggaagcct gcaccacctg gaaagcgatg
    38161 tataacggca ttaatgaact gattgatgac ctgatggatc acatcagcct ggaaaacaat
    38221 gtactgttcc cacgcgcgct ggcgggtgag tgatctaaaa gctggatggc attgcgccat
    38281 ccagcatgat aatgcggtta tttccgcagt tcagcagccc gcttcttacc aataaacagc
    38341 cagcccaacc ccagcgcgat aaaccacagc ggggtaacca gcagcgcctg gcgagtgtca
    38401 tcttccagtg tcagcaacac gaccacgaac acaaagaacg ccatacatac ccagcacatc
    38461 agcttgccga gcggcatctt gtagatcgac ttctcatgca gatgaggacg ctgtttgcgg
    38521 tacaccaggt acgagcaaag gataattgtc cagacgaaca taaacagaat cgcggaaacg
    38581 gttgtaatca tcgtgaacgc gccaatcacg ctaggattca catacaacat caccacgccg
    38641 ccgagcagac agatacacga gaaggtcaac cctttcgcgg gtactgcgcg cttagaaagt
    38701 ttagcgaacg ctttcggtgc tacaccttcc tgcgccagac caaacagcat acggctggta
    38761 gagaagacgc cgctgttagc ggaagacgcc gcagaggtca gcaccacaaa gttgatcacg
    38821 cttgcggcag caggcagccc taccaacacg aacagttcaa caaacgggct tttctccggg
    38881 actaccgaac tccacggcgt cacggacata attacaatca gcgcgaagac gtagaacata
    38941 atgatacgga tcggaataga gttaattgca cgcggcagtg atttctccgg atctttggtt
    39001 tccgcagcgg ttgtacctac cagctcaatc cctacgaaag cgaaaaccgc tatctggaat
    39061 ccggcaaaga agccacttaa acctttcggg aaccagccgc catcattcca caaatgcgcg
    39121 aatgacgctt ccacaccagt cggtgactga aagtgcatcg ccaccatgac caggccgacg
    39181 acaatcagcg acacaatggc gacgattttg atcatcgcaa accagaactc catctcaccg
    39241 aacattttca cggtggcgag attgagcgtc agcagcagca ctatcaccgc cagcgaggcg
    39301 acccagtcgg agagatcggg gaaccagaac tgagcataag cagtgatcgc caccacgtct
    39361 gccataccgg ttacaaccca gcagaaccag taagtccagc cggtgaaata tcctgcccac
    39421 ggcccgagta aatcggaagc gaagtcacta aaagatttgt attccagatt cgaaagcagc
    39481 aattccccca ttgcccgcat cacgaaaaag agcataaaac cgatgatcat ataaacgaaa
    39541 atgatcgacg gcccggcaag gctaatcgtt ttgccagacc ccataaacaa ccccgtacca
    39601 atggcaccgc caatggcaat aagctgaata tgtcggtttg tgagattgcg ccgtagcgac
    39661 tgttcagccg gagcctgatc atcggcaacg acttttacct gatctaccat gtttttttct
    39721 tcctgtacct gtctgtgttg ttcaggctct acggccttta gtcagtctat gatacgacga
    39781 atctgtatcg ggactcatcg atattaggta acaatacgcg ggatgaatac taagatttag
    39841 atctaatgtt aattttatgt ttaaagtgag tgtcatatca cggtgataac gcgaaacagc
    39901 tcacaaaaat acacgcttac ggggtctttg caacacaaaa tagcgccgtt tggcaaactg
    39961 aagggtttat tgctgaatgc ctgctcccct ctggttcgag gggaacagaa tgcgctgctt
    40021 agaggatttc cagcagttcg acttcaaaca ccagggtgct gaacggaggg atggatgcgc
    40081 ctgcgccgcg ctcgccatat gccagttcct gcgggatagt cagttcccat ttagaaccta
    40141 ccggcatcag agtcagtgct tcaatccagc cagggatcac gccattaacc ggaaattcag
    40201 cgggttcacc acgagcaacg gagctgtcaa acacggtgtc gtcgatcagt ttaccggtgt
    40261 aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc ttcaccctgg ttgatcacgc
    40321 ggaattgcag gccagattcg gtgctattca caccttcttt tttggcgttt tcttccaggt
    40381 atttcacacc ttcagcagcc atcgcctgga aacgctgacg acgaacggca tcggcgcgct
    40441 cgtggatttc acgcagcgcg cgatgcacca catcaaccgg aacagccgga tgtttgcctt
    40501 ccagcgcatc ggcaatacct gcaaccagtg cttctggcag cagcccttcc aggccagatt
    40561 cactcagttg ttgccctacc tgcaaaccaa tgccgtagct tgcttgcgct tcgatggtgt
    40621 caaaagttgg ggtggtcatc gtatttcctt tcttagggta taaaagtagc gggcagcata
    40681 tcagccacac ttctttgggt aaaactttgt cgcaggaatg atgacaaatc ccggggaaaa
    40741 agaaacaata gcaatatccc gtatgaatat aataaaggcg ggtttaccac ggacatcata
    40801 gcgttagctt attcataggc tatgattgag gaacaagacg cggagcagga ggaaaccatg
    40861 cccgggcgct ttgaactaaa accaaccctg gagaaagtct ggcacgcgcc ggacaatttt
    40921 cgcttcatgg acccgctgcc gccgatgcat cgtcggggga tcattattgc cgccattgta
    40981 ttggtggtcg gatttctgct tccatctgat gatacgccca acgcgccggt ggtgacgcgt
    41041 gaagcgcagt tagatattca gtcgcaatca caaccgccga cagaagaaca gctgcgggcg
    41101 caattagtca ccccacaaaa tgatcctgat caggtggctc cggtcgctcc agaacctata
    41161 caggaaggcc agccagaaga gcagccgcaa actacccaaa cgcaaccatt ccagccagat
    41221 agcggaattg ataatcaatg gcgctcttac cgtgtcgaac ccggtaaaac gatggcgcag
    41281 ctgttccgcg accacggact gcccgctacc gatgtctatg caatggcgca ggtagaaggc
    41341 gcgggtaagc cgctgagtaa tttgcaaaac gggcaaatgg tgaaaatccg ccagaacgcc
    41401 agcggcgtgg tgacgggctt aaccattgat acggggaata atcaacaggt cctgtttacc
    41461 cgccagccgg atggcagttt tattcgtgcg cggtagatga agggtcaacc aaaagccagc
    41521 taaggattgt ctctttgatg accttacgtc tggcttccag tgcctgggag gaaaccatac
    41581 tgatatgttg acatcctcca cgccctgaag gacgtggatc cctgcttcgt tcaggctgtc
    41641 gcctgaatca tttcggtggg ttcctgcttc aacgagcggc ctgactgcac catctctcca
    41701 caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccgtttac atcggcgttc
    41761 gctgtatatc cacatacctg gcatctgaat ttactttgtg acaggcg
//
LOCUS       DAZFRN010000025        41582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFRN010000025 DAZFRN010000000
VERSION     DAZFRN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.025"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(115..3264)
                     /gene="acrB"
                     /locus_tag="ACHKUB_001294"
     CDS             complement(115..3264)
                     /gene="acrB"
                     /locus_tag="ACHKUB_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO6654335.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3287..4480)
                     /gene="acrA"
                     /locus_tag="ACHKUB_001295"
     CDS             complement(3287..4480)
                     /gene="acrA"
                     /locus_tag="ACHKUB_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO6654336.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4622..5269
                     /gene="acrR"
                     /locus_tag="ACHKUB_001296"
     CDS             4622..5269
                     /gene="acrR"
                     /locus_tag="ACHKUB_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO6654337.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5397..8759
                     /gene="mscK"
                     /locus_tag="ACHKUB_001297"
     CDS             5397..8759
                     /gene="mscK"
                     /locus_tag="ACHKUB_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO6654338.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8820..8918
                     /locus_tag="ACHKUB_001298"
                     /pseudo
     CDS             8820..8918
                     /locus_tag="ACHKUB_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8971..9132)
                     /gene="rsmS"
                     /locus_tag="ACHKUB_001299"
     CDS             complement(8971..9132)
                     /gene="rsmS"
                     /locus_tag="ACHKUB_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO6654339.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9146..9673)
                     /gene="priC"
                     /locus_tag="ACHKUB_001300"
     CDS             complement(9146..9673)
                     /gene="priC"
                     /locus_tag="ACHKUB_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO6654340.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9743..10120
                     /gene="ybaN"
                     /locus_tag="ACHKUB_001301"
     CDS             9743..10120
                     /gene="ybaN"
                     /locus_tag="ACHKUB_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO6654341.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10273..10824
                     /gene="apt"
                     /locus_tag="ACHKUB_001302"
     CDS             10273..10824
                     /gene="apt"
                     /locus_tag="ACHKUB_001302"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO6654342.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10953..12884
                     /gene="dnaX"
                     /locus_tag="ACHKUB_001303"
     CDS             10953..12884
                     /gene="dnaX"
                     /locus_tag="ACHKUB_001303"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO6654343.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDSWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12217..12281
                     /gene="dnaX"
                     /locus_tag="ACHKUB_001303"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12937..13266
                     /gene="ybaB"
                     /locus_tag="ACHKUB_001304"
     CDS             12937..13266
                     /gene="ybaB"
                     /locus_tag="ACHKUB_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO6654344.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13266..13871
                     /gene="recR"
                     /locus_tag="ACHKUB_001305"
     CDS             13266..13871
                     /gene="recR"
                     /locus_tag="ACHKUB_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO6654345.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13981..15855
                     /gene="htpG"
                     /locus_tag="ACHKUB_001306"
     CDS             13981..15855
                     /gene="htpG"
                     /locus_tag="ACHKUB_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO6654346.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16036..16680
                     /gene="adk"
                     /locus_tag="ACHKUB_001307"
     CDS             16036..16680
                     /gene="adk"
                     /locus_tag="ACHKUB_001307"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO6654347.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16916..17878
                     /gene="hemH"
                     /locus_tag="ACHKUB_001308"
     CDS             16916..17878
                     /gene="hemH"
                     /locus_tag="ACHKUB_001308"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO6654348.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALRMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17875..18834)
                     /gene="aes"
                     /locus_tag="ACHKUB_001309"
     CDS             complement(17875..18834)
                     /gene="aes"
                     /locus_tag="ACHKUB_001309"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO6654349.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATETIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18986..20290
                     /gene="gsk"
                     /locus_tag="ACHKUB_001310"
     CDS             18986..20290
                     /gene="gsk"
                     /locus_tag="ACHKUB_001310"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO6654350.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20423..22099)
                     /gene="ybaL"
                     /locus_tag="ACHKUB_001311"
     CDS             complement(20423..22099)
                     /gene="ybaL"
                     /locus_tag="ACHKUB_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO6654351.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22337..23557)
                     /gene="fsr"
                     /locus_tag="ACHKUB_001312"
     CDS             complement(22337..23557)
                     /gene="fsr"
                     /locus_tag="ACHKUB_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO6654352.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23775..25427
                     /gene="ushA"
                     /locus_tag="ACHKUB_001313"
     CDS             23775..25427
                     /gene="ushA"
                     /locus_tag="ACHKUB_001313"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6654353.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25464..25943)
                     /gene="ybaK"
                     /locus_tag="ACHKUB_001314"
     CDS             complement(25464..25943)
                     /gene="ybaK"
                     /locus_tag="ACHKUB_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO6654354.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26147..26941)
                     /gene="ybaP"
                     /locus_tag="ACHKUB_001315"
     CDS             complement(26147..26941)
                     /gene="ybaP"
                     /locus_tag="ACHKUB_001315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO6654355.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27079..27420
                     /gene="ybaQ"
                     /locus_tag="ACHKUB_001316"
     CDS             27079..27420
                     /gene="ybaQ"
                     /locus_tag="ACHKUB_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6654356.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27534..30038)
                     /gene="copA"
                     /locus_tag="ACHKUB_001317"
     CDS             complement(27534..30038)
                     /gene="copA"
                     /locus_tag="ACHKUB_001317"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO6654357.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30300..31232
                     /gene="glsA"
                     /locus_tag="ACHKUB_001318"
     CDS             30300..31232
                     /gene="glsA"
                     /locus_tag="ACHKUB_001318"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO6654358.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31235..32527
                     /gene="ybaT"
                     /locus_tag="ACHKUB_001319"
     CDS             31235..32527
                     /gene="ybaT"
                     /locus_tag="ACHKUB_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO6654359.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            32558..32655
                     /locus_tag="ACHKUB_001320"
                     /pseudo
     CDS             32558..32655
                     /locus_tag="ACHKUB_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32652..33059
                     /gene="cueR"
                     /locus_tag="ACHKUB_001321"
     CDS             32652..33059
                     /gene="cueR"
                     /locus_tag="ACHKUB_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO6654360.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33060..33518)
                     /gene="ybbJ"
                     /locus_tag="ACHKUB_001322"
     CDS             complement(33060..33518)
                     /gene="ybbJ"
                     /locus_tag="ACHKUB_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO6654361.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVSAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33515..34432)
                     /gene="qmcA"
                     /locus_tag="ACHKUB_001323"
     CDS             complement(33515..34432)
                     /gene="qmcA"
                     /locus_tag="ACHKUB_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6654362.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34578..35255
                     /gene="fetA"
                     /locus_tag="ACHKUB_001324"
     CDS             34578..35255
                     /gene="fetA"
                     /locus_tag="ACHKUB_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO6654363.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35242..36021
                     /gene="fetB"
                     /locus_tag="ACHKUB_001325"
     CDS             35242..36021
                     /gene="fetB"
                     /locus_tag="ACHKUB_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO6654364.1"
                     /translation="MNSHNITNESLALALMLVVVAILISRKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36084..36938)
                     /gene="cnoX"
                     /locus_tag="ACHKUB_001326"
     CDS             complement(36084..36938)
                     /gene="cnoX"
                     /locus_tag="ACHKUB_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO6654365.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(36999..37808)
                     /gene="ybbO"
                     /locus_tag="ACHKUB_001327"
     CDS             complement(36999..37808)
                     /gene="ybbO"
                     /locus_tag="ACHKUB_001327"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6654366.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(37798..38421)
                     /gene="tesA"
                     /locus_tag="ACHKUB_001328"
     CDS             complement(37798..38421)
                     /gene="tesA"
                     /locus_tag="ACHKUB_001328"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO6654367.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            38392..39078
                     /gene="ybbA"
                     /locus_tag="ACHKUB_001329"
     CDS             38392..39078
                     /gene="ybbA"
                     /locus_tag="ACHKUB_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO6654368.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39075..41489
                     /gene="ybbP"
                     /locus_tag="ACHKUB_001330"
     CDS             39075..41489
                     /gene="ybbP"
                     /locus_tag="ACHKUB_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO6654369.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLLVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMMLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         9900 a        10937 c        11095 g         9650 t
ORIGIN      
        1 ggttaatact ggttttcgta tgagatcctg agttggtggt tcaattactc cttaatgttc
       61 gtaggttatg cataaaaaag gccgcttgcg cggccttagt gattacacgt tgtatcaatg
      121 atgatcgaca gtatggctgt gctcgatatc ttcattcttg cggctaaagc ggcggcgaac
      181 caccacaaag aataccggaa cgaagaagat tgccagtacc gttgcggtca ccatcccgcc
      241 cattacaccg gtacctactg cgttctgcgc gccggaacca gcaccagtac tgataaccag
      301 cggcataacg ccgaggataa acgccagcga ggtcatcagg atcggacgta aacgcatccg
      361 cactgcatca agcgtcgctt caatcagacc tttaccttct ttatccatca agtctttggc
      421 gaattcgacg ataagtatcg cgttcttcgc cgacaaccca atggttgtga gcaggcctac
      481 ctggaagtaa acgtcattgg tcaggccacg gaaggtggca gccagcaacg caccgataac
      541 ccccagcgga acgaccagca taacggagaa cggaatcgac cagctctcgt acagcgccgc
      601 cagacacagg aatacgacaa tcaacgaaat cgcgtacagt gaaggtgcct ggttgccgga
      661 gaggcgttcc tgataggaca tccccgtcca gtcatagcca acaccggtag gcagtttgct
      721 cgccagttgt tccattagct ccattgcttc accggtactt ttacccggtg ccgcctggcc
      781 taagatttcc atggatggca ggccattgta acgttccaga cgcggcgaac cgtactccca
      841 acgagaagag gagaatgccg agaacggcac catctgacca tcagcagcac gaacatacca
      901 gtcgccgata tcatccggca gcatacggta tttcgcttct gacatgacgt aaactttctt
      961 cacacgaccg cggtcgataa agtcgttcac atagctaccg ccccatgcag cgcccagagt
     1021 ggtgttaatg tcattgatag aaacacccag cgcctgcgct ttttcctggt cgatatcaat
     1081 cttaaactgc ggggtatctt ccagaccgtt tggacgtacg ctggtcaaca tatcagggtg
     1141 cttcgctgct tctgcaagca actggttacg cgcctgagtc agtttttcgt gaccaaggcc
     1201 agcctggtca atcagctcaa agtcaaagcc ggttgcagta cccagttcca cgattgcggg
     1261 caggttaaag gcgaaaacca tcgcatcttt gatttgcgag aaagcgcgtg ttgcacgcat
     1321 ggtaatcgct tcaactttgt tttcttcgcc cggacgatcg gcccagtcct tcaaggaaac
     1381 gaacgcaata ccggtattct gaccacgtcc cgcaaagccg aagccgttaa cggcgaacac
     1441 cgactcaacg ttgttctttt ctttggtcag atagtaatgc gttacctcat tgagcacttt
     1501 ctgcgtacgt tcctgcgttg cacctgctgg cagctgaacc atggtcataa atacgccctg
     1561 gtcctcatct ggcaagaagg agcttggcag acgcacgaac agataggcca tgccgaccac
     1621 gatgatcaga tacagcacca ggtaacgccc cgtactgcgc agaataccgc ctacgctgtc
     1681 ggtgtagtgg tgcgtgctct tctcgaacat gcggttaaac cagccgaaga agcctttttt
     1741 accttccccg tgatcgcctt tggcaatcgg tttcagcatg gtggcacaaa gagccggagt
     1801 caggatcaac gctaccagta ccgacagcgc cattgctgaa acaatggtaa tagagaactg
     1861 acgatagata gcaccagtag aaccgccaaa gaaggccatc ggtacgaata ccgctgacag
     1921 taccatcgcg ataccgacca gagcgccctg aatctgcccc atcgacttac gggtagcttc
     1981 ttttggcggc aaaccttctt ccgccataac acgctccacg ttttctacca caacgatagc
     2041 gtcatccacc aacaagccga tggcgagcac catcccgaac attgttagcg tgtttatcga
     2101 gaagccaaag gcggcaagga cggcaaaggt cccgagcaat accaccggta cggcaatggt
     2161 cggaatcaac gtcgcgcgga agttctgcag gaacagatac ataaccagga acacgaggat
     2221 gatcgcttcg accagcgttt taaccacttc gtgaatagag attttcacga acggcgtggt
     2281 gtcgtacggg taaacaattt tcagacccga cgggaagaac ggttccatct tcgccagttc
     2341 agcacggatt gccgcagcgg tatccagcgc gtttgcaccg gtcgccagct tgatccccag
     2401 accggaagcc ggttggccgt taaactctgc gatgatgtcg tagttctcac cacccagctc
     2461 aatcttcgcc acatcacgca gcagtacgcg ggaaccatcc tgattcactt tcagcaggat
     2521 tttgccgaac tcttcagtag aggtcagacg cgtctgagca ataatagagg cgttaagctg
     2581 ttggcctttc accggcggcg taccaccgag ctgacccgcc gcaacctggg cgttctgcgc
     2641 tttgatggcg gtaatgacat caaccggcgt tagctggaat ttgttcagct cattcgggtt
     2701 catccagata cgcatcgcgt actgtgaacc gaacaactga acatcaccca cgcccgacgt
     2761 acggctgatg gcatctttca tattcgccgc tacgtagtca gagatatcct cctgcgtcat
     2821 ggtgccatcg gtgttgataa cgccgacaac catcaggaag ctgctggatg atttctcaac
     2881 gctcacccct tgctgctgaa cttcttgcgg cagcaacggc atcgccagct gcagtttgtt
     2941 ctgcacctga acctgcgcga tatccgcatc agtaccagac tcaaaggtca gggtgatctg
     3001 cacggtaccc gtggagtcac tgttagagga catgtacatc aggttatcga taccgttcat
     3061 attctgttcg ataacctgtg tcaccgtgtc ctgcactgtt ttcgcatcag cgccggggta
     3121 ggaggcggag atcgttactg ccggcggtgc aatcgtagga tattgcgcca ccggcagttt
     3181 gaggatcgcc agcccccctg ccaacatgat gataatggcg atcacccacg caaaaatcgg
     3241 gcgatcgata aagaaattag gcatgtctta acggctcctg tttaagttaa gacttggact
     3301 gttcaggctg agcaccgctt gcggcttgct ggttattatc agcggtaact tcttgtgctt
     3361 ttacctggac accaggacgc actttctgca gcccacttat tactacgcga tcgcctgctt
     3421 tcagaccttc tgtcaccagc cacttatcgc caatagcctg gcttgcaacg atcggacggg
     3481 tttccacttt gtcatccgcg ccaactacca gtacggtggc atcaccacgc ggcgtacgag
     3541 ttacgccctg ttgcgggact aaaatagcgt ttggattaag cccttcttcc agacgtgcgc
     3601 gcacgaacat acccggcagc agagtgtgat ccgggttcgg gaagatagcg cgtagggtga
     3661 tagacccagt ggtctgatca acggtaacgt cagagaattc cagcgtaccg tcctgcggga
     3721 acttaatgcc gtcactggtg atcagcgaca ctttggcttt gccgttctct tgtttcagcg
     3781 tgccattcgc cagttcctgt ttcaggcgca ggaagtcgtt gctggactgg gtcacatcaa
     3841 cgtagatcgg atcaagttgt tgcacggttg ccagcgcagt cgcctgaccg ttctgtacca
     3901 atgcgccttc cgtcacgttc gacttaccaa tgcgaccgct aatcggagag gtgactttgg
     3961 tgtaagccag attgatccgc gcagtttcaa cggcagcttt cgccgcagtt accgcagcat
     4021 tcgcctgttg cgcatcagcc agagcctgat cgtactcttg cttactgatg tactgagtac
     4081 cgagcagttt ctgataacga ttcaccgtca attgcgcgat attggctgca gcctgggctt
     4141 tcgccagatc acctttcgca ctgtcgtatg tcgcctgata ggtcgcagga tcaatctgat
     4201 agagagagac accagcttcg atgtcgctac cttctttgaa attacgcttc aggataatcc
     4261 cgctaacttg aggacgaact tctgcgatcc ggtaggcact ggtgcgaccc ggaagctcgg
     4321 ttgtgatctg cagaggttca gttttgactg ttactacgcc aacggcgggc atctgctggc
     4381 caccttgttg ggcctgtttg tcgtcacatc ctgttagggc taagctgcct gagagcatca
     4441 gaacgaccgc cagaggcgta aaccctctgt ttttgttcat atgtaaacct cgagtgtccg
     4501 atttcaaatt ggtcaatggt caaaagttaa taaacccatt gctgcgttta tattatcgtc
     4561 gtgctatggt acatacattc acaaatgtat gtaaatctaa cgcctgtaaa ttcacgaaca
     4621 tatggcacga aaaaccaaac aagaagcgca agaaacgcgc caacacatcc tcgatgtggc
     4681 tctacgtctt ttctcacagc agggggtatc atccacctcg ctgggtgaga ttgcaaaagc
     4741 agctggcgtt acgcgcggtg caatctactg gcattttaaa gacaagtcgg atttgttcag
     4801 tgagatctgg gaactgtcag aatccaatat tggtgaacta gagcttgagt atcaggcaaa
     4861 attccctggc gatccactct cagtattaag agagatatta attcatgttc ttgaatccac
     4921 ggtgacagaa gaacggcgtc gattattgat ggagattata ttccacaaat gcgaatttgt
     4981 cggagaaatg gctgttgtgc aacaggcaca acgtaatctc tgtctggaaa gttatgaccg
     5041 tatagaacaa acgttaaaac attgtattga agcgaaaatg ttgcctgcgg atttaatgac
     5101 gcgtcgggca gcaattatta tgcgcggcta tatttccggc ctgatggaaa actggctctt
     5161 tgccccgcaa tcttttgatc ttaaaaaaga agcccgcgat tacgttgcca tcttactgga
     5221 gatgtatctt ctgtgcccca cgcttcgtaa tcctgccact aacgaataac cctgaatctg
     5281 actccaggat ttttcctgga cattttcgtc gttgctattc tggttcactg cgtcgtgata
     5341 ttcttgcggt ttgacttttt caggtcgttc ttcaggttca gaaaccttca ttcatcatga
     5401 ctatgttcca gtattacaaa cgatcacggc attttgtttt ttcagcattt attgcttttg
     5461 tttttgtctt gttatgccag aacacggcgt ttgcgcgggc gtcatcgaat ggtgatctgc
     5521 cgacaaaagc ggacctgcag gcgcaacttg actcactaaa taaacaaaaa gatctttctg
     5581 ctcaggacaa actggtgcag caggatctga cagatacatt agccaccctc gataaaatcg
     5641 atcgcataaa agaagagaca gttcagctac ggcaaaaagt cgctgaagcg ccggaaaaaa
     5701 tgcgccaggc gaccgcggcg ttaacagcac ttagcgatgt cgataacgac gaagaaacgc
     5761 gcaaaattct gagtacgctt tcgttgcgcc agctggaaac tcgcgttgcc caggcgctgg
     5821 acgatttgca aaacgcacaa aacgatctgg cgtcttataa cagccagctg gtttcgttac
     5881 agacgcagcc cgaacgcgtg caaaatgcga tgtataacgc ttcgcagcag ctgcaacaaa
     5941 ttcgcagtcg tctggatggg actgatgtcg gcgagactgc cttacgtccc agccagaaag
     6001 tgttaatgca ggctcagcag gcgttgctga atgcggagat tgaccagcag cgtaaaagcc
     6061 tggaagggaa caccgtcttg caggatacct tgcaaaagca acgtgattac gtgacggcga
     6121 acagcgcccg tctggagcac cagttgcagc tgttgcaaga agcggtaaac agcaagcgcc
     6181 tgactttaac tgaaaaaacg gcgcaggaag ccgtctcccc ggatgaagcc gcgcgtattc
     6241 aggctaatcc gctggtgaaa caggaactgg aaattaacca gcagttaagt cagcgtctga
     6301 ttaccgcgac tgaaaacggt aatcagttga tgcagcaaaa cattaaagtc aaaaactggc
     6361 tggagcgggc gctgcaatcg gaacgcaata ttaaagagca gattgccgtc ctgaagggca
     6421 gcctgctgtt gtctcgtatc ctttaccagc aacaacaaac gctgccctcg gcggatgaac
     6481 tggaaaacat gaccaaccgc attgcggatt tgcgtctcga acagtttgaa gttaaccagc
     6541 agcgtgatgc actcttccag agcgatgcgt tcgtcaacaa actggaagaa ggtcacacca
     6601 acgaagtcaa cagcgaagtt cacgatgcgt tattgcaagt ggttgatatg cgtcgcgaat
     6661 tgctggatca actcaacaaa cagttgggta accagctgat gatggccatt aacctgcaaa
     6721 tcaaccagca gcagttaatg agcgtatcga aaaacctgaa atccatcctg actcagcaaa
     6781 tcttttgggt gaacagtaac cgtccaatgg actgggactg gatcaaagcg ttcccgcaaa
     6841 gcctgaaaga tgaatttaag tcgatgaaaa tcacggtgaa ctgggaaaaa gcctggcctg
     6901 ccgtttttat cgctttcctc gccggtttgc cgctgctgtt gattgccggg ctgatccact
     6961 ggcgtctggg ctggctgaaa gcgtatcaac agaaactggc ttccgctgtg ggttccctgc
     7021 gtaacgatag ccagctcaac acaccaaaag cgatccttat cgacctgatc cgcgcgctgc
     7081 cggtgtgcct gattattctc gcggttggcc tgattctgtt gaccatgcag ctcaacatca
     7141 gcgaactgct atggtcgttc agtaaaaaac tggcgatttt ctggctggtg tttggcctgt
     7201 gctggaaggt actggagaaa aatggcgttg ccgtgcgtca cttcggcatg ccggagcagc
     7261 agaccagcca ctggcgtcgg caaattgtcc gcatcagtct cgcgttgctg cctatccatt
     7321 tctggtctgt ggtggcagaa ctttccccgc tgcatctgat ggatgatgtg ctggggcagg
     7381 cgatgatttt cttcaacctg ctgctgattg ccttcctggt atggccaatg tgccgcgaaa
     7441 gctggcgtga taaagagtcg cacaccatgc gactggtcac cattaccgtg ctgtcgatta
     7501 tcccgattgc attgatggtg ctgactgcca cgggttattt ctacaccacg ctgcgcctct
     7561 ccgggcgttg gattgaaacc gtttatctgg tgatcatctg gaacttgctc taccagacgg
     7621 tattgcgcgg cttaagcgta gcggcgcggc gtatcgcctg gcgtcgtgcg ctggcgcgtc
     7681 ggcagaatct ggtgaaagag ggcgcagaag gtgccgaacc gccggaagaa cccaccattg
     7741 cactggaaca ggttaaccag cagacgctgc gtattaccat gttgctgatg tttgcgctgt
     7801 tcggtgtcat gttctgggca atttggtccg atttgatcac cgtgttcagc tatctcgaca
     7861 gcatcacgct ctggcattac aacggcactg aagctggcgc tgcggtggtg aaaaacgtca
     7921 ccatgggcag tctgttgttt gcgattatcg cctcaatggt ggcctgggcg ttgattcgca
     7981 acctgcctgg tttactggaa gtgctggtgc tctcgcgact gaatatgcgt cagggcgcgt
     8041 cgtatgccat tactaccatc cttaactaca tcattattgc cgttggtgcg atgacggtgt
     8101 tcggatcgct gggcgtctcg tgggataaac tccagtggct ggcggcagca ttatccgtag
     8161 gtcttggttt tggcttacag gagatcttcg gtaactttgt ctctggtttg atcatcctgt
     8221 tcgaacgtcc ggtgcgtatt ggtgatacgg taaccattgg tagcttctcg gggacggtaa
     8281 gtaagatccg tattcgtgcg acaacgatta ccgatttcga tcgcaaagaa gtgatcatcc
     8341 cgaacaaagc gtttgttacc gagcgtctga tcaactggtc gttgactgac actactacgc
     8401 gtctggtgat ccgtctcggc gtggcctatg gctccgatct ggaaaaagtg cgtaaagtgt
     8461 tactgaaggc ggcgactgag cacccaaggg tgatgcacga accaatgccg gaagtcttct
     8521 ttacggcatt tggtgccagc acgttggatc atgagctgcg tctgtatgtg cgtgaactgc
     8581 gtgaccgtag tcgtactgtc gatgagctga accgtactat cgatcagctg tgccgtgaaa
     8641 acgacatcaa cattgccttt aaccagcttg aagtgcatct gcacaacgag aagggcgatg
     8701 aggtgacgga agtcaaacgc gactacaaag gcgatgaccc gacgccagcg gtagggtaat
     8761 aaccaaaggg ggcgacattt agttgccccg agattactga caaagtgcgc gttgttcatg
     8821 ccggatgcgg cgtgaccgcc ttatccggcc tacgaaaccg caagaattca atgtattgca
     8881 ggagcggtgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg cccacaatct
     8941 taggggacat ttagcggccc catttatttc tcacttttcc gcctcatcat cgcgcgttaa
     9001 tttcttttca taatcgcgtt ttacaatatc cagcgcgcgc agcacggtgc gggctgggat
     9061 ctgattttct tccagcagca caatcaaatc gacagccagt ttgacatcgt caggggcatt
     9121 ttccagtgac atactctctc cattgctagc gggttaagcg cgctagcctg ttttcgattt
     9181 tttccagcgc atggcggcag cgtgccaggc gcgcttcata ggcttccact tcacgatgca
     9241 gcgtttgctg ttccacgaga tcggtcaccc gcgccaggcg ggctctgcgt tcggcaacca
     9301 tctcacgtag ccgccgctca aaatcctgat gctgaatacg tttacgctgc cagcgggcaa
     9361 ttttcggtgg cgcactgtcc cactcgcgca atgaccaggc ggaggcttca cgcgcgatgg
     9421 cttccagttg tgccgccaga tgttccgcca gccaggccac ttgcggcagc tgttgctgct
     9481 caactgcatg acgaagcgca gccagattat cgcccgcctc atcgagacaa gcctgcagtg
     9541 ttgtcgcacg ggtttgaaaa aggtgtctgt caaaacgggc acttagcgtg gcaaattgcg
     9601 ctaccggggc acaacgctga cgcagcgtag cgagctgtcc ttccagtttt tccagcagca
     9661 gggcggtttt cacgataaga cctcaatgaa aatgataatt gttatgctaa agtagccact
     9721 ctgtaagctg acaacactca atatgcaacg aatcatttta atcatcattg gctggctggc
     9781 ggtagtgctg ggtacgctgg gcgtggtatt accggtatta ccgacgacgc cgtttatcct
     9841 gctggcggcc tggtgctttg cccgttcttc cccgcgcttt cacgcctggt tgctgtaccg
     9901 ctcatggttt ggcagctatc tacgtttctg gcagaaacat catgcgatgc cgcgcggcgt
     9961 caaaccgcgg gcgattttgc ttattttgct cacgtttgcc atttctctgt ggttcgtcca
    10021 gatgccatgg gtgcgcatca tgttgctggt aattctcgcc tgtttgcttt tctatatgtg
    10081 gcgaattccg gtgattgatg aaaagcaaga aaagcactga agcacaacat tcgcagttgc
    10141 aattattgcg tacagccagt acattctggc gttttcgagc acaggcgcag gcggtcaaag
    10201 gttaaacaac tgttactttt gatacgttta aaacgcgccg tgagtaccac cgtaacaagc
    10261 aggcatacac ttatgaccgc gactgcacag cagcttgagt atctcaaaaa tagcatcaaa
    10321 agcattcagg actacccaaa acccggcatt cttttccgcg atgtcaccag cttactggaa
    10381 gacccgaaag cttacgctct cagcatcgac ttgctggttg agcgttacaa aaatgcgggc
    10441 attaccaaag ttgtcggcac cgaagcgcgt ggcttcttgt ttggcgctcc ggtagctctg
    10501 ggtctgggcg ttggctttgt accggtccgt aaaccgggca aactgccgcg tgaaaccatc
    10561 agtgaaactt acgacctgga atacggcacc gatcagctgg agatccacgt tgatgccatc
    10621 aaaccgggcg acaaagttct ggtggtggac gatctgctgg caaccggcgg cactatcgaa
    10681 gcgaccgtta aactgatccg tcgtctgggt ggtgaagtgg ctgacgctgc gttcattatc
    10741 aacctgttcg atctcggcgg cgaacagcgt ctcgaaaaac agggcattac cagctacagc
    10801 cttgtcccgt tcccgggcca ttaattatcg ccagtcttgt gctgcccacg ctacggacag
    10861 cacaagatgt gcattcagcc tcgccgttct gacggggctg tgttagcatt accccttcgt
    10921 gaatccacct tccagcgttt cagagcctgc caatgagtta tcaggtctta gcccgaaaat
    10981 ggcgcccaca aacctttgct gacgtcgtcg gccaggaaca tgtgctgacc gcactggcga
    11041 acggcttgtc gttagggcgt attcatcatg cttatctttt ttccggtacc cgtggcgtcg
    11101 gaaaaacctc tatcgcccga ctgctggcga aggggctaaa ctgcgaaacc ggcattaccg
    11161 cgacgccgtg cggcgtgtgc gataactgtc gtgaaatcga gcaggggcgc tttgtcgatc
    11221 tgattgaaat cgacgccgcc tcgcgcacca aagttgaaga tacccgcgac ctgctggata
    11281 acgtccagta cgctccggcg cgtggtcgtt tcaaagttta tctcatcgac gaagtgcata
    11341 tgctgtcgcg ccacagcttt aacgcactgt taaaaactct tgaagagccg ccggagcatg
    11401 ttaagtttct gctggcgacg accgatccac agaaattgcc ggtgacgatt ttgtcacgct
    11461 gtctgcaatt tcatctcaag gcgctggatg tcgagcaaat tcgccatcag cttgagcaca
    11521 tcctcaacga agaacatatc gctcacgagc cgcgggcgct gcaattgctg gcacgcgccg
    11581 ctgaaggcag cctgcgagat gccttaagtc tgaccgacca ggcgattgcc agtggtgacg
    11641 gccaggtttc aacccaggcg gtcagtgcga tgctgggtac gcttgacgac gatcaggcgc
    11701 tgtcgctggt tgaagcgatg gtcgaggcca acggcgagcg cgtaatggcg ctgattaatg
    11761 aagccgctgc ccgtggtatc gagtgggaag cgttgctggt ggaaatgctc ggcctgttgc
    11821 atcgtattgc gatggtacaa ctttcgcctg ctgcacttgg caacgacatg gccgccattg
    11881 agctgcggat gcgtgaactg gcgcgcacca taccgccgac ggatattcag ctttactatc
    11941 agacgctgtt gattggtcgc aaagaattac cgtatgcgcc ggaccgccgc atgggcgttg
    12001 agatgacgct gctgcgcgcg ctggcgttcc atccacgtat gccgctgcct gagcctgaag
    12061 tgccacgcca gtcctttgca cccgtcgcgc caacggcagt aatgacgcca acccaggtgc
    12121 cgccgcaacc gcaatcagcg ccgcagcagg caccgactgt accgctcccg gaaaccacca
    12181 gccaggtgct ggcggcgcgc cagcagttgc agcgcgtgca gggagcaacc aaagcaaaaa
    12241 agagtgaacc ggcagccgct acccgcgcgc ggccggtgaa taacgctgcg ctggaaagac
    12301 tggcttcggt caccgatcgc gttcaggcgc gtccggtgcc atcggcgctg gaaaaagcgc
    12361 cagccaaaaa agaagcgtat cgctggaagg cgaccactcc ggtgatgcag caaaaagaag
    12421 tggtcgccac gccgaaggcg ctgaaaaaag cgctggaaca tgaaaaaacg ccggaactgg
    12481 cggcgaagct agcggcagaa gccattgagc gcgactcgtg ggcggcacag gtgagccaac
    12541 tttcgctacc aaaactggtc gaacaggtgg cgttaaatgc ctggaaagag gagagcgaca
    12601 acgcagtatg tctgcatttg cgctcctctc agcggcattt gaacaaccgc ggtgcacagc
    12661 aaaaactggc tgaagcgttg agcatgttaa aaggttcaac ggttgaactg actatcgttg
    12721 aagatgataa tcccgcggtg cgtacgccgc tggagtggcg tcaggcgata tacgaagaaa
    12781 aacttgcgca ggcgcgcgag tccattattg cggataataa tattcagacc ctgcgtcggt
    12841 ttttcgatgc ggagctggat gaagaaagta tccgccccat ttgatcgtaa gcacagctta
    12901 cgttcgtcat ccttaacgtg attgagagag aaacctatgt ttggtaaagg cggtctgggt
    12961 aacctgatga agcaagccca gcagatgcaa gaaaaaatgc agaaaatgca ggaagagatc
    13021 gcgcagctgg aagttaccgg cgaatctggc gcaggtctgg taaaagtgac catcaacggt
    13081 gcacacaact gccgtcgcgt agagatcgac ccgagcctgc tggaagacga caaagagatg
    13141 ctggaagacc tggtggctgc agcattcaac gacgcagcac gtcgtattga agaaacgcag
    13201 aaagaaaaaa tggcctctgt atcctccgga atgcagctgc cgcctggctt taagatgccg
    13261 ttctgatgca aaccagcccg ctgttaacac agcttatgga agcactgcgc tgtctgccgg
    13321 gcgttggccc gaagtcggcg cagcgtatgg cgttcacgct gcttcagcgc gatcgtagcg
    13381 gcgggatgcg tctggcgcag gcgctcaccc gggcgatgtc ggaaatcggc cactgcgccg
    13441 attgccgcac tttcaccgaa caggaagtct gtaacatctg ttcgaatccg cgtcgtcagg
    13501 aaaacggtca aatctgtgtg gtggagagtc cggcggacat ctacgccatt gagcagacgg
    13561 ggcagttttc aggccgttat tttgtgttga tggggcatct gtcaccgctg gacggcatcg
    13621 gtccggatga tatcgggctt gatcgtctgg aacagcgtct ggcagaggaa aaaatcactg
    13681 aagtgatcct cgccaccaat cccacggttg aaggtgaagc taccgctaac tacattgccg
    13741 agctttgcgc gcaatatgac gtggaagcca gccgaatcgc tcatggcgta ccggttggcg
    13801 gcgaactgga aatggtcgat ggcaccacgc tgtcacactc ccttgccggg cgtcataaga
    13861 ttcgttttta agcaaacgag agcaggatca cctgctctcg cttgaaatta ttctcccttg
    13921 tccccatctc tccctcatcc tgtttttaac cttaaaatgg cattattgag gtagacctac
    13981 atgaaaggac aagaaactcg tggttttcag tcagaagtga aacagcttct gcacctgatg
    14041 atccattctc tctattccaa taaagaaatc ttcctgcgtg agcttatctc taacgcctcc
    14101 gatgcggcgg acaagctgcg tttccgtgcg ctatctaacc cggacctgta cgaaggtgat
    14161 ggcgaactgc gcgttcgtgt ctctttcgat aaagacaagc gcacgctgac catctccgat
    14221 aacggcgtgg ggatgacccg cgacgaagtg attgaccatc tggggactat cgctaaatcc
    14281 ggtaccaaat cattcctcga atccctgggt tctgaccagg cgaaagacag ccagctgatc
    14341 ggtcagtttg gtgttggttt ctactctgcg tttatcgtgg ccgacaaagt gaccgtgcgt
    14401 actcgcgcgg caggcgaaaa accagaaaac ggcgtcttct gggaatcggc tggcgaaggt
    14461 gaatataccg ttgccgacat caccaaagaa gatcgtggta ctgaaatcac cctgcatctg
    14521 cgtgaaggcg aagacgagtt cctcgatgac tggcgcgtgc gttccatcat cagcaaatac
    14581 tccgaccata tcgcgctgcc ggtagagatc gaaaaacgcg aagagaaaga cggcgaaacc
    14641 gttatctcct gggagaaaat caacaaagcg caggcgctgt ggactcgtaa caagtcggaa
    14701 atcaccgatg aagagtacaa agagttctac aaacacatcg cccacgactt taatgatccg
    14761 ctgacctgga gccacaaccg cgttgaaggt aagcaggagt acaccagcct gctgtacatc
    14821 ccgtcccagg ctccgtggga tatgtggaac cgcgatcata aacacggcct gaaactgtac
    14881 gtccagcgtg tgttcatcat ggacgacgca gaacagttca tgccgaacta tctgcgcttc
    14941 gtgcgtggtc tgattgactc cagcgatctg ccgctgaacg tttcccgtga aatcctccag
    15001 gacagcacgg taacgcgtaa cctgcgcaat gcgctgacca agcgtgtgct gcaaatgctg
    15061 gaaaaactgg cgaaagacga cgcggaaaaa taccagacct tctggcaaca gtttggcctg
    15121 gtactgaaag aaggtccggc ggaagatttc gctaaccagg aagcgatcgc caaactgctg
    15181 cgttttgctt ctacttatac cgattcttct gcgcagaccg tgtctctgga agactacgtt
    15241 tcccgcatga aagaagggca ggagaaaatc tactacatca ctgcagacag ctatgcggca
    15301 gcgaagagca gcccgcacct ggaactgttg cgtaagaaag gcatcgaagt tctgctgctt
    15361 tccgaccgca tcgatgagtg gatgatgaac tatctgactg agttcgacgg taaaccgttc
    15421 cagtcggtgt ctaaagttga cgagtcgctg gaaaaactgg ctgacgaagt tgatgagagc
    15481 gcgaaagaag cggagaaagc actgactccg ttcatcgacc gtgtgaaagc cctgctcggc
    15541 gagcgcgtga aagatgtccg tctgactcac cgtctgaccg atacgccagc gatcgtttcg
    15601 accgacgcgg acgaaatgag cactcagatg gctaaactgt tcgctgcggc gggccagaaa
    15661 gtgccagaag tgaaatacat cttcgaactg aacccggatc acgtactggt gaaacgtgcg
    15721 gcagatactg aagatgaagc gaagttcagc gagtgggtag aactgctgct ggatcaggcg
    15781 ctgctggcag aacgcggcac gctggaagat ccgaacctgt ttattcgtcg tatgaaccag
    15841 ctgctggttt cctgatgtca tgccggatga cctttgtgtc atccggcatt tttcttttca
    15901 tcatctgcac tttccgcaaa ttatctcgcc attaaccgtt tcagcctcag gtgcctttct
    15961 tgaggcaatc gcctgttggt ggtatcgttt atcgcttttt caaaaaattc gacacatttt
    16021 aaggggattt tcgcaatgcg tatcattctg cttggcgctc cgggcgcggg gaaagggact
    16081 caggctcagt tcatcatgga gaaatatggt attccgcaaa tctccactgg cgatatgctg
    16141 cgtgctgcgg tcaaatctgg ctccgagctg ggtaaacaag caaaagacat tatggatgct
    16201 ggcaaactgg tcaccgacga actggtgatc gcgctggtta aagagcgcat tgctcaggaa
    16261 gactgccgta atggtttcct gttggacggc ttcccgcgta ccattccgca ggcagacgcg
    16321 atgaaagaag cgggcatcaa tgttgattac gttctggaat tcgacgtacc ggacgaactg
    16381 attgttgatc gtatcgtagg ccgccgcgtt catgcgccgt ctggtcgtgt ttatcacgtt
    16441 aaattcaatc cgccgaaagt agaaggcaaa gacgacgtta ccggtgaaga actgactacc
    16501 cgtaaagacg atcaggaaga aaccgtgcgt aaacgtctgg ttgaatacca tcagatgact
    16561 gcaccgctga tcggctacta ctccaaagaa gcggaagcgg gtaacaccaa atacgcgaaa
    16621 gttgacggca ccaagccggt tgctgaagtt cgcgctgatc tggaaaaaat cctcggctaa
    16681 ttcgaaagcg cgcacggaca gtcccctcgc cccctcgggg agagggttag ggtgagggga
    16741 acaggcccgc acaagcaaac ttatcagcaa tctcaggccg gatattcatt cggcctttta
    16801 caaaaacctc aatctgcgct attccttttt ctgatttgac ctctcacagc aattagttct
    16861 tcttcctcac ttttccgcta caattatcaa caagttgaat cgataagagg cggtaatgcg
    16921 tcagactaaa accggtatcc tgctggcaaa cctgggtacg cccgatgccc ccacacctga
    16981 agcggtaaaa cgctatctga aacaattttt aagcgacaga cgcgtggttg atacctcacg
    17041 gttgttatgg tggccgttgc tgcgcggcgt gattttgccg ctgcgctcgc cgcgtgtggc
    17101 gaagctgtac gcctcggtct ggatggaaga cggctcgccg ctgatggttt acagccgcca
    17161 gcaacagcag gcgctggcac aacgtttacc ggagacgccc gtagcgctgg gcatgagcta
    17221 cggctcgcca tcactggaaa gcgccgtcga tgaactcctg gcagagcatg tagatcatat
    17281 tgtggtgctg ccgctttatc cgcaattctc ctgttctacg gtcggtgcgg tatgggatga
    17341 actggcacgc attctggcgc gcaaacgtag cattccgggg atatcgttta ttcgtgatta
    17401 cgccgataac cacgattaca ttaatgcact ggcgaacagc gtacgcgctt cttttgccaa
    17461 acatggcgaa ccggatctgc tgctgctctc ttatcatggc attccccagc gttatgcaga
    17521 tgaaggcgat gattacccgc aacgttgccg cacaacgact cgcgaactgg cttccgcatt
    17581 gaggatggca ccggaaaaag tgatgatgac ctttcagtcg cgctttggcc gggaaccctg
    17641 gctgatgcct tataccgacg aaacgctgaa aatgctcgga gaaaaaggcg taggtcatat
    17701 tcaggtgatg tgcccgggct ttgctgcgga ttgtctggag acgctggaag agattgccga
    17761 gcaaaaccgt gaggtcttcc tcggtgccgg cgggaaaaaa tatgaatata ttccggcgct
    17821 taatgccacg ccggaacata tcgaaatgat ggctaatctt gttgccgcgt atcgctaaag
    17881 ctgagcggta aagaactgag cgccgtcgcg aagagcctcg tcggcggttt tcatcatccg
    17941 tgaataatgc aaaaaggcgt gcagcgtgcc tgggtagagt ttgaactcac agggctgctg
    18001 atgcgccgct aacgtctggt aaagcagacg gctgtcatcc agcagcggat cgaactccgc
    18061 cccggcaata aaacagggcg gaacttcgcg agtgagatca ttattaaaca gacagtaata
    18121 cggtgactcg cggtccgcgt cgttgcttaa atacgcctct tcatacatct gcaaatcctg
    18181 ttgcgttaag ccatcccaga caccgcccaa cagacgacga gtcacggaat cccgtaatcc
    18241 gtaaagccca taccacagca aaacacccgc aattttaccg caatcgatct gtttatcacg
    18301 caaccacaac gcactggcga gcgccagcat ggcacctgcg gaatcaccgg caaagccaat
    18361 gcgggacata ttgatttgat aatcctccgc ctgctggtgg aaataacaac aagcagccac
    18421 aatttcctct atcgcttgcg gaaaacgcgc ttcaggtgaa agggggtaat caataccaat
    18481 caccgtacat tggctgtagc ttgccagcag gcgcatgatg cgatcgtggg tatcgagatt
    18541 gccgagaata aaaccgcctc catgcaaata aaatagcgtc gccgggctat ctggctgcgg
    18601 acaaaagaga cgtgtttcca cctgcccata ttttgttgga accatgtaag ctctggttgc
    18661 catttctgga gcgcccgcat tccagaatcg gcgctcaagc gtgtaatact gtcgttgctc
    18721 agcaatcgtt tccgttgcgg gccaggacgg taaatccggc tgaagagtat tcacaacggt
    18781 cttcatttca gcagaaataa ggtccagaac aggtagtttg ttttccggct tcatacaaaa
    18841 actcctttca aattacgtca ttgtaaggaa ccactgccat gaaaatgcga tcccgcctgc
    18901 tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga ttgtgttaac attcgccgct
    18961 cagttaacca cccgtaaaaa caaccatgaa atttcccggt aaacgtaaat ccaaacatta
    19021 cttccctgta aatgcacgcg atccgctgct tcagcaattc cagccagaaa acgaaaccag
    19081 cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat attgaagcga aagtggatga
    19141 tgaattcatt gagcgttatg gattaagcgc cgggcattca ctggtgattg aggatgacgt
    19201 agccgaagcg ctttatcagg agctaaaaca gaaaaacctg attacccatc agtttgcggg
    19261 tggcaccatt ggtaacacta tgcacaacta ctcggtgctc gcggacgacc gttcggtgct
    19321 gctgggcgtc atgtgcagca atattgaaat tggcagttat gcctatcgtt acctgtgtaa
    19381 cacttccagc cgtaccgatc ttaactatct acaaggcgtg gatggcccga ttggtcgttg
    19441 ctttacgctg attggcgagt ccggggaacg tacctttgct atcagtccag gccacatgaa
    19501 ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc ggagcctcgg cactggttct
    19561 cacctcatat ctggtgcgtt gcaagccggg tgaacccatg ccggaagcaa ccatgaaagc
    19621 cattgagtac gcgaagaaat ataacgtacc ggtggtgctg acgctgggca ccaagtttgt
    19681 cattgccgag aatccgcagt ggtggcagca attcctcaaa gaccacgttt ctatccttgc
    19741 gatgaacgaa gatgaagccg aagcgttgac cggagaaagc gatccgttgt tggcatctga
    19801 caaggcgctg gactgggtag atctggtgct gtgcaccgcc gggccaatcg gcttgtatat
    19861 ggcgggcttt accgaagacg aagcgaaacg taaaacccag catccgctgc tgccgggcgc
    19921 tatagcggaa ttcaaccagt atgagtttag ccgcgccatg cgccacaagg attgccagaa
    19981 tccgctgcgt gtatattcgc acattgcgcc gtacatgggc gggccggaaa aaatcatgaa
    20041 cactaatgga gcgggggatg gcgcattggc agcgttgctg catgacatta ccgccaacag
    20101 ctaccatcgt agcaacgtac caaactccag caaacataaa ttcacctggt taacttattc
    20161 atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc tatcaggtac tgaaccagca
    20221 ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac agcctggaag agtcttactg
    20281 ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc gttcacgccg catctggcat
    20341 ttggctctcg atgcccgatg cgacgctggc gcgtcttatc atgcctacat atttttcata
    20401 ttttacatcc ggcaaccacc gtttaccccg tcaccacttc acccgccggt ggcgtttcca
    20461 gcagttccag catagtacgg gcaatttcac gttcacccat cactacctga ttcgcgccac
    20521 gttcggtgat atacgtcact tcatcgtcat aatgggcgcg ggcgataatc tcaatatccg
    20581 ggtttttcgc gcgggcagat gccacaatct cgcctgcttc ataaccgttg ggaatcgtca
    20641 ggatcagcca ttttgcacat tccagatgcg ccagttgcat aatttcttcg ttcgccgcat
    20701 tgcccaatac tgcgcggacc ccgcgctctc gcagctcatc aacacgggtt cgtgacgtct
    20761 caatcaccac cagcggaata tcagaggcga gcaatttctc ccccagcagg ctgcctacac
    20821 gaccgtaacc caccagaagc gcatggttgc aaatatccac tgggatctgc ttctcttctt
    20881 cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt cttcgccaga tatttctcca
    20941 gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat cgcccctgcc agtaccaggt
    21001 tttgtccggc ctgcggcagt aaattcaatg ccattcccag tcccgccagg ataaacgcga
    21061 actcaccaat ctgcgccagg ctggcggcga tggttaatgc cgtacgttgg gagtgaccaa
    21121 acagtcgcac caggaaaaat gcggctaacg acttaccaaa cagaataatc gccagcgtcg
    21181 ccagcactgc cagcggttgc tgaatcagaa ttaacggatc aaacaacatc ccgacggaga
    21241 caaaaaacag caccgcaaac gcgtcgcgca atggcagcgt atcgtgggcg gcacggtgac
    21301 tcagttcaga ctcgttcagt accatcccgg caaagaacgc accgagtgca aaggagacat
    21361 caaacagctc taccgcacca aaggcaatcc ctaacgccag cgccagcacc gacagggtaa
    21421 acagctcgcg agaaccggtt gccgcgctgc gtgccataat ccacggcacc agacggcgac
    21481 ctaccagcat cataatagcg ataaatgcga tcactttgcc gatggtgatc cccatatcga
    21541 ctgcaagagt ggcaaagccc acatcgccct gttccatcat tcctgccact gcgggcagca
    21601 acaccagcgt cagaaccatt accaggtctt ccacaatcaa ccaaccgatg gcgatttgcc
    21661 cacgctgact gtcaattaat tgccgttctt caagtgcgcg cagtaacacc acggtactgg
    21721 cggtggaaag acataaaccg aacacgatac cggtcattaa cgaccagccc agcacggcag
    21781 agagcgccat acccagcagc gtcgccacgg ctatctgggc gatcgcaccg ggaatggcga
    21841 tggcctttac cgccatcaaa tccttcagcg aaaagtgcaa accgacgcca aacatcaaca
    21901 gaatgacgcc cagttcagcc agttccggcg caagcttggt atcggcaaca aagcccggag
    21961 tgaatggtcc tgccagcaca cccgctaaca gatatcccac cagaggagaa atacgtagtt
    22021 tattggccag catgccgagg ataaaggcga gcacaaggcc gccaacaatg gtggtgataa
    22081 gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg ttattgtcca tttttggccg
    22141 ggaaaaccaa aattacaggt aatagtttat gacaatttca ttgatgatgt tcatgaataa
    22201 ttattgaatt ttgcagaaaa atggaattag ctgcaaaaaa agcacggatc ggaaaacaga
    22261 aggcggttta acagaggaaa ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc
    22321 tttggctgca atgaaatcag tctttatgcc ggttatcagg caggaatatg gtcaacatcc
    22381 ccaatagtgg caggaaagca cagattttat agactaactc gatgctggtg tgatcggcga
    22441 taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg aaaaagagtc
    22501 cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga atggcagaga
    22561 atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag tgcaggctgg
    22621 cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa atcacatatt
    22681 tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca acggcaaaca
    22741 ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt tgcatcagat
    22801 aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa atgaggatta
    22861 acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt atcgtcgctt
    22921 tggattttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt tgcgccaaca
    22981 ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg ccataaggcg
    23041 cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg ccgacctgaa
    23101 agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg cgagaagatt
    23161 ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga actgcgccaa
    23221 aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg ccaattggca
    23281 acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc agtagcgaag
    23341 aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga gaaaattctg
    23401 actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc agcagatgtg
    23461 agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt gacgcagccg
    23521 cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt tttcagggtt
    23581 gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct gtatgcgtcg
    23641 cctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga tttatcttca
    23701 ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt tgaaatcagg
    23761 tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg ttaaccacat
    23821 ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac aaaattacag
    23881 ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc gaatatggtc
    23941 tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct gaaggcggta
    24001 gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct gacttacagg
    24061 atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg gcgatcggta
    24121 atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg gccaagttcc
    24181 cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt aaaccgtggg
    24241 cgctgtttaa gcgtcaggat ctgaaaattg ccgtcattgg gctgacaacc gatgacacag
    24301 caaaaattgg taacccggaa tacttcactg atgtcgaatt tcgtaagccc gccgatgaag
    24361 cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt atcgcggcga
    24421 cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc gatgtggaga
    24481 tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac tcgcaagatc
    24541 cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt acgccatgca
    24601 agccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc aaatacgtgg
    24661 gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac cagctgattc
    24721 cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc gtgctttaca
    24781 ctcctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcaccgttc cagaacaaag
    24841 gcaaagcgca gctggaagtg aaaataggcg aaaccaatgg tcgtctggaa ggcgatcgtg
    24901 acaaagtgcg ttttgtacag actaatatgg ggcggttgat tctggcagcc caaatggatc
    24961 gcactggtgc cgactttgcg gtgatgagcg gaggcggaat tcgtgattct atcgaagcag
    25021 gcgatatcag ctataaaaac gtgctgaaag tgcagccatt cggcaatgtg gtggtgtatg
    25081 ccgacatgac cggtaaagag gtgattgatt acctgaccgc cgtcgcgcag atgaagccag
    25141 attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac ggcaaactga
    25201 acgaccttaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt atggcgacat
    25261 taaacttcaa tgccaccggc ggtgatggat atccgcgcct tgataacaaa ccgggctatg
    25321 tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa agctcgccgc
    25381 tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc gaaagtgccg
    25441 gatgtttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa tttggcatcg
    25501 aggatctttg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt gccgccggaa
    25561 acataaatag tggcaaattc ttgtgcaggg gcgtcgataa ttgttggcag acgttttttc
    25621 tgccccagtg ggctaatccc ccctaccaga taccccgtcg aacgctgcgc ggccatcgga
    25681 tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag atccagttga
    25741 cctgcgaccg gcgtaacggc cacggcaagg tgtttcatat caccgttcac tgccaccagc
    25801 agcgttttgt agacctgatc cggattcaaa cctaattttt tgacgacttc atcgccaaaa
    25861 ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt gtttttttcg
    25921 agtaatttaa ctgcgggtgt catggtaatt ttcctgcttt aaacaaaaaa acgaagcaag
    25981 catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat tggcaggatg
    26041 gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata ataataaaaa
    26101 aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag cgcaacattt
    26161 gcggtaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca tatcgccccg
    26221 gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc agcacatcgt
    26281 acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcggc ggtgcattca
    26341 gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg gtcagcgtat
    26401 cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggcaatc tggttttcag
    26461 ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat aactggtaat
    26521 cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga agaaccatcg
    26581 cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc tcctggctaa
    26641 tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc tcgcaagtag
    26701 gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc agcgcatcgg
    26761 cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg ctacccatat
    26821 gaatactgcc aatcagatga aaatggcgat tgccggggag ggtgatatcg atggcaggcc
    26881 aggagtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac aacagatcca
    26941 tacgacctcc ctttgtgaaa tatcatgcta gcgcgcgggg agggatggcg caaatggagt
    27001 tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct attcacaggg
    27061 aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc cggagatatt
    27121 cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc agagttgctg
    27181 catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact caccactgag
    27241 atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct aaacctccag
    27301 gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga attgggacgg
    27361 attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa ggtcgcgtaa
    27421 ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt ttacgccgca tccggcagtc
    27481 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    27541 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    27601 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    27661 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    27721 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    27781 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    27841 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    27901 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    27961 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    28021 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    28081 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    28141 acaggcgttg ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca
    28201 acctgttgct catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca
    28261 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca
    28321 cctgctttat cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc
    28381 gccagacgca atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc
    28441 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    28501 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    28561 gaaataatcg acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc
    28621 gtggtggcaa tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt
    28681 gccgcactga ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc
    28741 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt
    28801 gacagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacgctacc gtcctgtacc
    28861 actgtcccgg catggacgct ttcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    28921 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    28981 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ccgccagagg cacgcttttt
    29041 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagc
    29101 gccttagaag aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg
    29161 ataatcatcg cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc
    29221 cacaggttga cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta
    29281 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    29341 gcgaaaacca tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca
    29401 gcggtgacca tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc
    29461 agtgcaacaa ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg
    29521 cgctcgcggc gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc
    29581 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    29641 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    29701 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    29761 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    29821 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    29881 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    29941 tgttccacat ccgggcgctg ttcgagactt tctttcacgc gtttaacgca gtgaccgcag
    30001 gacaggccgt ccagggtcag gtcgataatt tgtgacataa aacactcctt taagacagtt
    30061 ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa
    30121 ccaggcgagt aaaagtccgt aaagattaaa aaattggctc aatttgcatc aggattagac
    30181 atttatctct ttgttttcct gaagttaagt tgcgggtgct aagttaaatc aagatgcctg
    30241 aaaatcggca ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    30301 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    30361 ttaacggcgg acaaaatgcc gactacattc cctttctggc gaatgtacct agtcaactgg
    30421 cagcagtggc tattgtgacc tgcgatggca acatctatcg cgcgggtgat agtgattacc
    30481 gctttgcgct ggaatccatc tcgaaagtct gtacgttagc ccttgcgtta gaggatgtcg
    30541 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    30601 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcca
    30661 ttgccaccac cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac
    30721 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    30781 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    30841 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctcc acgctcctca
    30901 atactgttga actggcaaca cttggcgcga cgctggcggc ggggggggtg aatccgctga
    30961 cgcatgaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    31021 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    31081 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    31141 caccgctgga cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    31201 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    31261 taacaaacct ctcggtctat ggaacgtcgt ttccatcggc attggggcaa tggtgggggc
    31321 ggggatcttc gcgctgctgg ggcaggctgc attgctaatg gaagcctcga cctgggtcgc
    31381 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    31441 gagctatccc agcaatggcg gcattatcga cttctttcgt cgcggattag gcaacggcgt
    31501 cttttcgctg gcgctctcgt tactgtacct gttgacgctg gcggtgagca tcgccatggt
    31561 cgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    31621 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    31681 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    31741 attgctgatt attgccggtg tctggtcgtt gcaaccggcg catatttctg tctctgcgcc
    31801 ccccagctcc ggtgcgttct tctcctgtat cgggataacc ttccttgcct atgcgggctt
    31861 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    31921 gtttctggtg gcaattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    31981 tagcgatgta tcggcattag agttagaaaa atatgccgat accgccgtag cgcaggcagc
    32041 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    32101 ggcttcagcc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    32161 cgaacgcgaa ctaccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    32221 catcattgtc gtggtgttga ttatgctgat gacggcggca ctgaatttag gctcactcgc
    32281 cagcgttgcc agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg
    32341 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    32401 gttggtgatc gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg
    32461 gattattggc tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac
    32521 cgtataacat ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    32581 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    32641 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    32701 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    32761 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    32821 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    32881 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    32941 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    33001 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    33061 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc
    33121 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    33181 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    33241 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    33301 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acatcacccc ttgccactcc
    33361 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgacac gccgctccac
    33421 agcaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    33481 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    33541 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    33601 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    33661 tctgcgctac gaagtagttc accgcctgaa tatcaccgga ggcaatggct tcagatacca
    33721 ttttggtggc gcgggcttct gcttcggcag aacgttcacg cgcttcggcc tgtaagaatg
    33781 ccgactgacg ttcaccttcc gctttcagaa tttgcgactg tttttcacct tccgctttga
    33841 gtatttccgc ctgacggatc ccttccgctt caaggatgta agcgcgtttg gtacgttccg
    33901 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    33961 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    34021 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    34081 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag
    34141 ccgcgcgcgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    34201 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    34261 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    34321 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    34381 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    34441 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    34501 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    34561 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    34621 atctggcggg tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    34681 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    34741 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    34801 agccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    34861 atacggtata cgataatctg atctttccct ggcagatccg taaccagcag cctgacccgg
    34921 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaaaaata
    34981 tcgccgagct atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta
    35041 tgccgaaggt tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata
    35101 acgttaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    35161 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    35221 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attggcactg
    35281 gcattaatgc tggtggtggt ggcaatctta attagccgta aagaaaaact ggcgctggag
    35341 aaagatattt tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    35401 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    35461 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    35521 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctct
    35581 gggtcgattg aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac
    35641 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    35701 caacagattc aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg
    35761 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    35821 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    35881 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    35941 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    36001 acgcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    36061 ttcatgtgcc gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    36121 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    36181 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    36241 tcattgcgcc caacctggtg cagttgcaga gccagttgcg tcgccagtgc ggcatcttct
    36301 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc
    36361 agcagttcga tttgcgccac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    36421 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    36481 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    36541 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    36601 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    36661 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    36721 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    36781 ttgtactgcg ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt
    36841 tcagaccaaa aatagaacag taccggagtg gtcatcgact gttcaagaac ctgttgcagg
    36901 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    36961 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    37021 atcacgcgcc ccggcagcag gcgcttaagc accattactg cccaggtcac cagcgttacc
    37081 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc caccaccgct
    37141 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    37201 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    37261 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    37321 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    37381 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    37441 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    37501 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    37561 agggcgatca cctcgtctgc tgcgcgatca acactttctg gtgaatccag atcgatcaac
    37621 acgccggtaa atcccatgtt gttcatacgc tcaacatcat ccggttttcg gcaacctgcc
    37681 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    37741 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    37801 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    37861 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    37921 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    37981 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    38041 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    38101 ctgtggctga aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccagcgcgg
    38161 ctgatgctgt ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct
    38221 gatgctggca ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca
    38281 ggccgcgctg gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt
    38341 gtccgctgcg gcggcacgga aggttaacag gaccaggaac aggaagggca aatgccagcg
    38401 gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc
    38461 tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc
    38521 gagtcgggat cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc
    38581 agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca
    38641 aagttgcgcg cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt
    38701 aacgcgctgg aaaacgtcga gcttccggcg ctgctgcgcg gtgagagtag cgcggaaagt
    38761 cgtaacggag cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct tgatcatctt
    38821 ccggcacagc tttccggcgg cgaacagcaa cgagtggcgc tggcgcgagc ctttaatggt
    38881 cgacctgatg tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat
    38941 aaaattgccg acttgctgtt ttccctcaac cgtgagcatg gcaccacgtt gattatggtg
    39001 acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag
    39061 ttgcaggagg aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat
    39121 taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca
    39181 gcgatcgcat ggagaagggc ttaagccaac aaagccggga gtttatggcg ggcgatcggg
    39241 cgttgcgcag ttcgcgcgaa gtgccgcaag catggctgga ggaagcgcaa aagcgcggcc
    39301 tgaaagtcgg caagcagctg actttcgcca ccatgacatt tgcaggcgac acaccgcagc
    39361 tggcgaacgt caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc
    39421 cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcgctgc
    39481 ttaacctgaa aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag
    39541 aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga
    39601 tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc
    39661 gttataaatt cggcggcaac gagaaccagc tcgacggcta tgagaaatgg ttgttacccc
    39721 agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat
    39781 cgatggaacg ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag
    39841 tggcagcagt ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga
    39901 tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt
    39961 tgctggtgct ggcgctttca gccgttaccg gtggggcgat aggcctgttg ttcgaaaacg
    40021 tgttgatggt gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc
    40081 cgtggctatg ggcgctgggc accatgacgg ttatctcgct gctggtgggg ctacgaccgt
    40141 accgcttgtt gctggcaacg cagcctttac gcgtattacg taatgatgtg gtcgcgaacg
    40201 tctggccgct gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg
    40261 gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt
    40321 tgctgtgcgg tgtgctgggc tggatgatgc tgaatgtact tcgccgcatg acgctgaaat
    40381 cgctacctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acattaagcc
    40441 agctttcggc attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg
    40501 atctgcttga ccgctggcag cagcagctac ctccagaaag cccgaactac tttttaatta
    40561 acattgccac agaacaggta gaaccgctaa aagcgttcct cgcggaacat cagatagtcc
    40621 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag
    40681 aaggtaatga agatgaggcg cttaaccgcg aactcaatct tacctggcaa aatacgcgac
    40741 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    40801 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg
    40861 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    40921 ggcctaattt ctattttatt ttccctgaag gggcattaga cgggcaaccg cagagctggc
    40981 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    41041 ctaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
    41101 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    41161 tgctggcaca ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca
    41221 cactcggtgc ggggaaaaaa ctgctgcgta cgacattgtg gtgcgagttc gccatgcttg
    41281 ggtttgtttc tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg
    41341 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    41401 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg cgcgagactg gttaagggta
    41461 aggcgctgtt caggcagttt gcggggtgat gagagtaaat aaggggcgtt ctggttgaat
    41521 ggaacgcctt gtttaaatta attctacaat atgtttcatt gatgatgtta ttgaattggt
    41581 ga
//
LOCUS       DAZFRN010000026        41240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFRN010000026 DAZFRN010000000
VERSION     DAZFRN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.026"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            174..1073
                     /gene="lysO"
                     /locus_tag="ACHKUB_001331"
     CDS             174..1073
                     /gene="lysO"
                     /locus_tag="ACHKUB_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO6654370.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1217..2869
                     /gene="hcp"
                     /locus_tag="ACHKUB_001332"
                     /gene_synonym="priS"
     CDS             1217..2869
                     /gene="hcp"
                     /locus_tag="ACHKUB_001332"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO6654371.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2881..3849
                     /gene="hcr"
                     /locus_tag="ACHKUB_001333"
     CDS             2881..3849
                     /gene="hcr"
                     /locus_tag="ACHKUB_001333"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO6654372.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3982..5700
                     /gene="poxB"
                     /locus_tag="ACHKUB_001334"
     CDS             3982..5700
                     /gene="poxB"
                     /locus_tag="ACHKUB_001334"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO6654373.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHAEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5737..6738
                     /gene="ltaE"
                     /locus_tag="ACHKUB_001335"
     CDS             5737..6738
                     /gene="ltaE"
                     /locus_tag="ACHKUB_001335"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO6654374.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6749..8179
                     /gene="ybjT"
                     /locus_tag="ACHKUB_001336"
     CDS             6749..8179
                     /gene="ybjT"
                     /locus_tag="ACHKUB_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO6654375.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8278..9291
                     /gene="ybjS"
                     /locus_tag="ACHKUB_001337"
     CDS             8278..9291
                     /gene="ybjS"
                     /locus_tag="ACHKUB_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6654376.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9288..10118)
                     /gene="amiD"
                     /locus_tag="ACHKUB_001338"
     CDS             complement(9288..10118)
                     /gene="amiD"
                     /locus_tag="ACHKUB_001338"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO6654377.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10115..10438)
                     /gene="ybjQ"
                     /locus_tag="ACHKUB_001339"
     CDS             complement(10115..10438)
                     /gene="ybjQ"
                     /locus_tag="ACHKUB_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO6654378.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            10564..11079
                     /gene="ybjP"
                     /locus_tag="ACHKUB_001340"
     CDS             10564..11079
                     /gene="ybjP"
                     /locus_tag="ACHKUB_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6654379.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            11297..12025
                     /gene="artP"
                     /locus_tag="ACHKUB_001341"
     CDS             11297..12025
                     /gene="artP"
                     /locus_tag="ACHKUB_001341"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO6654380.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            12043..12774
                     /gene="artI"
                     /locus_tag="ACHKUB_001342"
     CDS             12043..12774
                     /gene="artI"
                     /locus_tag="ACHKUB_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO6654381.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            12781..13497
                     /gene="artQ"
                     /locus_tag="ACHKUB_001343"
     CDS             12781..13497
                     /gene="artQ"
                     /locus_tag="ACHKUB_001343"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO6654382.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            13497..14165
                     /gene="artM"
                     /locus_tag="ACHKUB_001344"
     CDS             13497..14165
                     /gene="artM"
                     /locus_tag="ACHKUB_001344"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO6654383.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            14457..15188
                     /gene="artJ"
                     /locus_tag="ACHKUB_001345"
     CDS             14457..15188
                     /gene="artJ"
                     /locus_tag="ACHKUB_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO6654384.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(15363..16490)
                     /gene="rlmC"
                     /locus_tag="ACHKUB_001346"
     CDS             complement(15363..16490)
                     /gene="rlmC"
                     /locus_tag="ACHKUB_001346"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO6654385.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(16531..17019)
                     /gene="ybjO"
                     /locus_tag="ACHKUB_001347"
     CDS             complement(16531..17019)
                     /gene="ybjO"
                     /locus_tag="ACHKUB_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO6654386.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(17079..17924)
                     /gene="potI"
                     /locus_tag="ACHKUB_001348"
     CDS             complement(17079..17924)
                     /gene="potI"
                     /locus_tag="ACHKUB_001348"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO6654387.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(17921..18874)
                     /gene="potH"
                     /locus_tag="ACHKUB_001349"
     CDS             complement(17921..18874)
                     /gene="potH"
                     /locus_tag="ACHKUB_001349"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO6654388.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(18884..20017)
                     /gene="potG"
                     /locus_tag="ACHKUB_001350"
     CDS             complement(18884..20017)
                     /gene="potG"
                     /locus_tag="ACHKUB_001350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO6654389.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(20112..21224)
                     /gene="potF"
                     /locus_tag="ACHKUB_001351"
     CDS             complement(20112..21224)
                     /gene="potF"
                     /locus_tag="ACHKUB_001351"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO6654390.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFLIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(21576..22052)
                     /gene="ybjN"
                     /locus_tag="ACHKUB_001352"
     CDS             complement(21576..22052)
                     /gene="ybjN"
                     /locus_tag="ACHKUB_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO6654391.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(22140..23042)
                     /gene="rimK"
                     /locus_tag="ACHKUB_001353"
     CDS             complement(22140..23042)
                     /gene="rimK"
                     /locus_tag="ACHKUB_001353"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO6654392.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(23103..23825)
                     /gene="nfsA"
                     /locus_tag="ACHKUB_001354"
     CDS             complement(23103..23825)
                     /gene="nfsA"
                     /locus_tag="ACHKUB_001354"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO6654393.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCFGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(23809..24096)
                     /gene="ybjC"
                     /locus_tag="ACHKUB_001355"
     CDS             complement(23809..24096)
                     /gene="ybjC"
                     /locus_tag="ACHKUB_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO6654394.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            24256..24513
                     /gene="grxA"
                     /locus_tag="ACHKUB_001356"
     CDS             24256..24513
                     /gene="grxA"
                     /locus_tag="ACHKUB_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO6654395.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(24543..24920)
                     /gene="ybjM"
                     /locus_tag="ACHKUB_001357"
     CDS             complement(24543..24920)
                     /gene="ybjM"
                     /locus_tag="ACHKUB_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO6654396.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            25190..26875
                     /gene="ybjL"
                     /locus_tag="ACHKUB_001358"
     CDS             25190..26875
                     /gene="ybjL"
                     /locus_tag="ACHKUB_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO6654397.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(27036..27578)
                     /gene="rcdA"
                     /locus_tag="ACHKUB_001359"
     CDS             complement(27036..27578)
                     /gene="rcdA"
                     /locus_tag="ACHKUB_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001631241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO6654398.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVVDKF"
     gene            27662..28870
                     /gene="ybjJ"
                     /locus_tag="ACHKUB_001360"
     CDS             27662..28870
                     /gene="ybjJ"
                     /locus_tag="ACHKUB_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6654399.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            28870..29685
                     /gene="ybjI"
                     /locus_tag="ACHKUB_001361"
     CDS             28870..29685
                     /gene="ybjI"
                     /locus_tag="ACHKUB_001361"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO6654400.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            29771..30055
                     /gene="ybjH"
                     /locus_tag="ACHKUB_001362"
     CDS             29771..30055
                     /gene="ybjH"
                     /locus_tag="ACHKUB_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO6654401.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(30096..31328)
                     /gene="mdfA"
                     /locus_tag="ACHKUB_001363"
     CDS             complement(30096..31328)
                     /gene="mdfA"
                     /locus_tag="ACHKUB_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO6654402.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIQQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            31613..32209
                     /gene="ybjG"
                     /locus_tag="ACHKUB_001364"
     CDS             31613..32209
                     /gene="ybjG"
                     /locus_tag="ACHKUB_001364"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6654403.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVIIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            32267..33025
                     /gene="deoR"
                     /locus_tag="ACHKUB_001365"
     CDS             32267..33025
                     /gene="deoR"
                     /locus_tag="ACHKUB_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO6654404.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(33072..34274)
                     /gene="dacC"
                     /locus_tag="ACHKUB_001366"
     CDS             complement(33072..34274)
                     /gene="dacC"
                     /locus_tag="ACHKUB_001366"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6654405.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            34521..35147
                     /gene="gstB"
                     /locus_tag="ACHKUB_001367"
     CDS             34521..35147
                     /gene="gstB"
                     /locus_tag="ACHKUB_001367"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO6654406.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(35144..36259)
                     /gene="yliI"
                     /locus_tag="ACHKUB_001368"
     CDS             complement(35144..36259)
                     /gene="yliI"
                     /locus_tag="ACHKUB_001368"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO6654407.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(36370..36753)
                     /gene="bssR"
                     /locus_tag="ACHKUB_001369"
     CDS             complement(36370..36753)
                     /gene="bssR"
                     /locus_tag="ACHKUB_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO6654408.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            36966..38291
                     /gene="rimO"
                     /locus_tag="ACHKUB_001370"
     CDS             36966..38291
                     /gene="rimO"
                     /locus_tag="ACHKUB_001370"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO6654409.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(38338..39666)
                     /gene="dgcI"
                     /locus_tag="ACHKUB_001371"
     CDS             complement(38338..39666)
                     /gene="dgcI"
                     /locus_tag="ACHKUB_001371"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6654410.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(39674..>41240)
                     /locus_tag="ACHKUB_001372"
     CDS             complement(39674..>41240)
                     /locus_tag="ACHKUB_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO6654411.1"
                     /translation="VPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYFMT
                     TRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIER
                     SNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVR
                     KLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHK
                     SAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHA
                     MEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGY
                     QIIKTAMGEFRHFSQRASLKDDFLLHINVSHWQLNEPHFHERFTTIMKENGLKANSLC
                     VEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQF
                     TGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMP
                     FSAWDKSGKLVKE"
BASE COUNT        10100 a        10747 c        10305 g        10088 t
ORIGIN      
        1 tttatatggc attgcacatg aaaggagcgc aacaatgcta aacactcaga taggattaaa
       61 aatccccctc tgatgcataa catttccttt taattgcaac tttactcgtc cttccgcttt
      121 cagtagtgtg tcatcgcgca tttcgacttc atctttgcaa ggactgggtt ttcatgtttt
      181 ctgggctgtt aatcattctg gttcccctga ttgtgggtta cctcattccg cttcgccaac
      241 aagctgcgtt aaaagttatt aatcagctat taagctggat ggtttacctt attctctttt
      301 ttatgggtat aagtctggcg tttctcgata acctcgccag taacctgttg gcgattctgc
      361 attattctgc cgtcagtatt accgttattt tactgtgtaa tattgccgcc ctgatgtggc
      421 tggagcgagg cctgccgtgg cgcaaccacc atcagcaaga aaaactcccg tcgcgtattg
      481 cgatggcgct ggagtcgcta aaactgtgcg gcgtagtagt gattggtttt gccattggtc
      541 tgagtggact ggctttctta caacacgcga ccgaagccag tgaatacacg ttaattttgc
      601 tacttttcct cgttggtatt cagttgcgca ataatggcat gaccttaaag cagattgtcc
      661 ttaatcgccg gggaatgatt gtcgccgtgg tggtggttgt cagttcatta attggtggtt
      721 taattaacgc ctttattctt gatctcccca tcaataccgc gctggcaatg gcctccggtt
      781 tcggctggta ttctctttcc ggtattttat tgaccgaatc ttttggtccg gtaatcggga
      841 gcgcggcgtt ttttaatgat ctggcccgtg aactgattgc tattatgttg atccctgggc
      901 tgattcgccg cagccgctct actgcactgg gcttatgcgg tgccacatca atggatttca
      961 ccctgcccgt tcttcaacgt actggcgggc tggatatggt cccggcggca attgttcacg
     1021 gttttattct tagcctgtta gtgccgatcc tcatcgcctt tttctctgcg taatacctct
     1081 ctggcggtag atccctgccg ccaaaattgc gctaaatcaa tctcccttaa agttgcatga
     1141 aaaatccctt ttatccccgc gttaagcgtc ttaaccttaa acatgtatat taaatataac
     1201 tttaaaaggt gtgatcatgt tttgtgtgca atgtgaacaa actatccgta ctccggcagg
     1261 aaacggctgc tcatacgcgc aggggatgtg tggtaaaacg gcggaaactt ctgaccttca
     1321 ggatttactc atcgcggcgc tgcaagggct ttctgcctgg gcggtaaaag cgcgtgaata
     1381 cggcatcatc aaccacgatg ttgacagctt cgcgccacgt gcctttttct caaccctgac
     1441 caacgttaac ttcgattctc cgcgtattgt cggctacgct cgtgaagcga ttgccctgcg
     1501 cgaggcgctg aaagcgcaat gcctggctgt agatgccaac gcgcgcgtcg ataacccgat
     1561 ggctgacctg caactggtga gcgacgatct cggcgagctg caacgtcagg cagcagaatt
     1621 tacccctaac aaagataaag cggcgattgg cgaaaacatt ctcggcctgc gtctgctgtg
     1681 cctgtatggc ctgaaaggtg cggcggccta tatggaacac gcgcatgttc tcggtcaata
     1741 cgacaacgat atttatgccc agtatcataa aatcatggcg tggctgggga cctggcctgc
     1801 cgatatgaac gcgcttcttg agtgttcaat ggaaatcggc cagatgaact tcaaagtgat
     1861 gagcattctg gatgcaggcg aaaccggtaa atacggacac ccgacgccaa cccaggtcaa
     1921 cgtcaaagcg acggcgggta aatgcatcct gatttccggt cacgatctca aagatctcta
     1981 caacctgctg gaacagaccg aaggcacggg cgttaatgtt tacacccacg gcgaaatgct
     2041 gcctgcgcat ggctacccgg agctgcgtaa attcaagcat ctggtcggta actacggcag
     2101 cggctggcag aatcagcaag tggagttcgc tcgtttccct ggccccatcg tgatgacctc
     2161 gaactgcatc atcgacccaa ccgtaggcgc ttatgacgat cgtatctgga cccgcagcat
     2221 cgttggctgg ccgggtgtgc gtcatctgga tggcgaagat ttttctgcgg tcatcgccca
     2281 ggcgcaacaa atggcgggct tcccgtacag cgaaattccg cacctgatca ccgtgggctt
     2341 tggccgccag acgctgcttg gcgcagcgga tacgctgatt gatctggtga gccgtgaaaa
     2401 actgcgtcat atcttcctgc ttggtggctg tgacggcgca cgcggcgagc gccactactt
     2461 caccgatttc gccaccagcg tgccggatga ctgcctgatc ctgaccctcg cctgtggtaa
     2521 atatcgcttt aacaaacttg agtttggcga tatcgaaggt ctgccgcgtc tggtagatgc
     2581 aggtcaatgt aacgatgctt actcagcgat tattctggct gtcactctgg cagagaaact
     2641 gggctgcggt gtcaacgatc tgccgctgtc gctggtgctc tcctggtttg aacagaaagc
     2701 gatcgtcatt ctgctgacgc tgctttctct gggcgtgaaa aatatcgtca ccggcccgac
     2761 tgcacctggt ttcctgacac cagacctgct ggcggtgctg aacgagaaat tcggcctgcg
     2821 ttctatcacc actgttgaag aagacatgaa gcaactgttg agcgcgtaag gagtttgtcg
     2881 atgacgatgc caacgaatca atgcccgtgg cggatgcagg ttcatcacat tacgcaagaa
     2941 acgccggatg tgtggacgat ttccctgatt tgccacgatt actacccata tcgcgccggg
     3001 caatatgcgc tggtcagcgt acgaaactca gcggaaacgc tgcgtgctta caccatttcc
     3061 tccacgccag gcgtgagtga atacatcacc ctgaccgtgc ggcggattga tgacggtgtc
     3121 ggctcccagt ggctgacgcg cgatgtaaaa cgcggtgatt atctctggct ttcggacgcg
     3181 atgggggaat ttacctgcga cgataaagca gaagataaat tcctgctgct ggcggcaggc
     3241 tgcggcgtca cgccgattat gtcgatgcgt cgctggctgg cgaagaatcg tccacaggcc
     3301 gatgtacagg tgatctacaa cgtgcgtacg ccgcaggatg ttattttcgc cgatgagtgg
     3361 cgtaactatc cggtaacgct ggtggcggaa aataacgtta ccgaaggctt tatcgctggt
     3421 cgtctcactc gcgaactgct gactcgcgtt cctgacttag cttcacgtac cgtgatgacc
     3481 tgcggcccgg ctccgtatat ggattgggta gagcaggaag tgaaagcgct cggcgtgacg
     3541 cgtttcttta aagagaaatt cttcacccca gtagcggaag cggcgaccag cggtctgaaa
     3601 ttcaccaaac tgcaaccggc acgagaattt tacgccccgg ttggcaccac gctactggag
     3661 gcgttggaaa gcaataacgt tccggttgtc gctgcctgcc gtgcgggtgt ttgcggctgc
     3721 tgtaagacga aagtggtttc cggtgaatat acggtgagca gcacaatgac gctgaccgac
     3781 gccgaaatcg ctgaaggtta cgtactggcc tgctcctgcc atccgcaggg ggatttggtt
     3841 ctcgcataat cgccttatgc ccgatgatat tcctgtcatc gggctattta accgttagtg
     3901 cctcctttct ctcccatccc ttccccctcc gtcagatgaa ctaaacttgt taccgttatc
     3961 acattcagga gatggagaac catgaaacaa acggttgcag cttatatcgc caaaacactc
     4021 gaatcggcag gcgtgaaacg catctgggga gtcacaggcg actctctgaa cggtcttagt
     4081 gacagtctta atcgcatggg caccatcgag tggatgtcca cccgccatga agaagtggcg
     4141 gcgtttgccg ctggcgctga agcacaactt tcaggagaac tggcggtctg tgccggatcg
     4201 tgcggccccg gcaacctgca cttaatcaac ggcctcttcg attgccaccg caatcacgtt
     4261 ccggtactgg ctattgccgc tcatattccc tccagcgaaa ttggcagcgg ctatttccag
     4321 gaaacccacc cgcaagagtt attccgcgaa tgtagccact attgcgagct ggtttccagc
     4381 ccggagcaga tcccacaagt actggcgatt gccatgcgca aagcggtgct taaccgtggc
     4441 gtttcggttg tcgtgttacc aggcgacgtg gcgttaaaac ctgcgccaga aggggcaact
     4501 acccactggt atcatgcgcc acagccagtc gtgacgccgg aagaagaaga gttacgcaaa
     4561 ctggcgcaac tgctgcgtta ttccagcaat atcgccctga tgtgtggcag cggctgcgcg
     4621 ggagcgcata aagagttagt tgagtttgcc gggaaaatta aagcgcctat tgttcatgcc
     4681 ctgcgcggta aagagcatgc cgaatacgat aatccgtatg atgtcggaat gacgggatta
     4741 atcggcttct cgtcaggttt ccataccatg atgaatgccg atacgttagt gctgctcggc
     4801 acgcaatttc cctaccgcgc cttctacccg accgatgcta aaatcattca gattgatatc
     4861 aacccagcca gcatcggcgc tcacagcaag gtggatatgg cgctggtcgg cgatatcaaa
     4921 tcaaccctgc gggcattgct gccactggtg gaagaaaaaa ccgatcgcaa gtttctggat
     4981 aaagcgctgg aagattaccg cgacgcccgc aaagggctgg acgatttagc taaaccgagc
     5041 gagaaagcca ttcacccgca atatctggcg caacaaatta gtcattttgc cgccgatgac
     5101 gccatcttta cctgtgacgt cggcacgcca acggtatggg cggcacgtta tctgaaaatg
     5161 aacggcaagc gtcgcctgtt aggttcgttt aaccacggtt cgatggctaa cgccatgccg
     5221 caggcgctgg gtgcgcaggc gacagagcca gaacgtcagg tggtcgccat gtgcggcgat
     5281 ggcggtttta gcatgttgat gggcgatttc ctctcagtag tgcagatgaa actgccagtg
     5341 aaaattgtcg tctttaacaa cagcgtgctg ggctttgtgg cgatggagat gaaagctggt
     5401 ggctatttga ctgacggcac cgaactacac gacacaaact ttgcccgcat tgccgaagcg
     5461 tgcggcatta cgggtatccg tgtagaaaaa gcgtctgaag ttgatgaagc cctgcaacgc
     5521 gccttctcta tcgacggtcc ggtgttggtg gatgtggtgg tcgccaaaga agagttagcc
     5581 attccaccgc agatcaaact cgaacaggcc aaaggattta gcttgtatat gttgcgcgca
     5641 atcatcagcg gacgcggcga tgaagtgatc gaactggcga aaacgaactg gctaaggtaa
     5701 aaagggtggc atttcccgtc ataataagga catgccatga ttgatttacg cagtgatacc
     5761 gttacccgac cgagccgcgc catgctcgaa gcgatgatgg ccgccccggt tggggacgac
     5821 gtttacggag acgaccctac cgttaatgct ctgcaggact atgcagcaga gctttccggt
     5881 aaagaagccg ccatttttct gcctaccggc actcaggcca acctggtcgc tctgctcagt
     5941 cactgcgaac gtggcgaaga gtatattgtc ggtcaggccg cgcataacta tctgtttgaa
     6001 gccggtggcg cagcggtgct gggcagtatt cagccgcaac ccatcgacgc ggctgccgac
     6061 ggcacgctac cgctggataa agtggcgatg aaaatcaaac ccgacgatat ccatttcgcc
     6121 cgcaccaaat tactcagtct ggaaaacacc cacaacggca aagtgctgcc gcgtgaatac
     6181 ctgaaagaag catgggaatt tacccgcgag cgcaatctgg cgctgcatgt tgacggtgcg
     6241 cgcatcttta atgccgtggt ggcttacggc tgcgaactga aagagatcac gcaatattgt
     6301 gattcgttca ccatttgcct gtcgaaaggt cttgggacgc cagtcggttc attactcgtc
     6361 ggtaatcgtg attacattaa acgtgccatc cgctggcgga aaatggccgg tggcgggatg
     6421 cgtcagtccg ggattctggc tgccgccggg atgtatgccc tgaaaaataa cgtcgcacgg
     6481 ttgcaggaag atcacgacaa cgccgcctgg atggcggagc aactgcgtga agcaggcgcg
     6541 gatgtgatgc gtcaggacac taatatgctg tttgttcgcg tcggggaaga aaatgctgcc
     6601 gcgttaggcg aatacatgaa agcgagaaac gtgctgatta acgcctcgcc gattgtccgc
     6661 ctggtgacgc atcttgacgt ctcgcgcgaa caactggcgg aagtcgccgc ccactggcgc
     6721 gcattcctgg cgcgttaagg agagagacgt gccgcaacgc attttagttc tcggtgccag
     6781 tggctacatt ggtcagcatc tggtgcgcac actcagccag caagggcatc agatcctggc
     6841 ggcggcacgt catgtcgaca ggcttgcaaa gctgcaactg gcaaacgtca gttgccataa
     6901 agtcgatctc agctggccgg ataaccttcc ggccctgttg caggatatcg ataccgtcta
     6961 ttttctggtg cacagcatgg gcgaaggcgg cgattttatc gctcaggagc gccaggtggc
     7021 tctcaacgtc cgcgatgcgc tacgtgaagt accagttaag caattaatct ttctcagttc
     7081 gttgcaggcc ccgccacatg agcagtcgga tcatctgcgt gctcgtcagg ctacggcgga
     7141 cattcttcgt gaagcgaatg tacctgtgac cgaacttcgg gccggaatta tcgttggcgc
     7201 aggttcagcg gcgttcgaag tcatgcgcga tatggtctac aacctgccag tgttaacgcc
     7261 gccacgctgg gtacgttcac gcaccacgcc catcgcgctg gaaaacttgc tgcactatct
     7321 ggtggcgttg ttagatcatc cggccagcga acaccgcatc ttcgaagccg ccggaccaga
     7381 ggtgctcagt tatcagcaac agtttgaaca ttttatggcg gtgagcggta agcgccgctg
     7441 gttgatcccc atccccctcc ccacccgctg gatttcggtg tggtttctca atgtgattac
     7501 ttccgtaccg cccaccaccg ccagggcgtt gattcagggg ctgaaacacg atctgctggc
     7561 ggatgatacc gcgctacgtg cactcatccc acaacggctg atcgctttcg atgacgcggt
     7621 acgtagcacg ttgaaagagg aggaaaaact ggtcaactcc agcgactggg gctacgacgc
     7681 tcaggccttt gcccgctggc gaccggagta cggttatttt gccaaacagg cggggtttac
     7741 cgttaaaacg tccgccagcc ttgctgcttt atggcaggta gtgaaccaaa tcggcggtaa
     7801 agagcgttat ttctttggca atattttgtg gcagacacgg gcgttgatgg accgcgcgat
     7861 cggtcataag ctggcgaaag gccgcccgga gcgcgaatat ttacagactg gcgatgcggt
     7921 agatagctgg aaagtgattg tcgttgaacc ggaaaaacaa cttacgttgt tatttggcat
     7981 gaaagcgccg gggctgggac gactgtgttt tagcctggaa gataaaggcg actatcgtac
     8041 tatcgatgtc cgcgctttct ggcatccgca cggtatgccg gggctgtttt actggttatt
     8101 gatgatcccc gcgcatctgt ttatttttcg cggaatggca aaacaaatcg ccagactggc
     8161 agaacaaagc acagattaat cgacaaaacc tgcgttattc tttcatgttt cccattgcat
     8221 catgggggaa atcacggaag aatgcgcacg ttattttctc aacaacagtg gattgatatg
     8281 aaggtactgg ttaccggcgc caccagcggc ttaggtcgaa acgcggtaga gtttttatgc
     8341 cagaaaggca tcagcgtgcg agcgaccggt cgcaacgagg caatgggcaa attgctggaa
     8401 aaaatgggcg cagagtttgt tccggcggat ctgaccgagc tggtttcatc acaagctaaa
     8461 gtgatgctcg cgggcattga tacgctgtgg cactgctcca gctttacctc accctggggg
     8521 acacaacagg ctttcgatct ggctaacgtt cgcgccactc gccgcctggg tgaatgggct
     8581 gtcgcctggg gtgtgcgtaa ctttattcat atctcttccc cctccctgta cttcgattat
     8641 caccaccatc gcgatattaa agaagatttt cgccctcacc gcttcgccaa cgagtttgcc
     8701 cgcagcaaag cagccagcga agaagtgatc aatatgcttt cgcaggcgaa tccacaaacg
     8761 cgctttacta ttctgcgccc acaaagtctg ttcggaccgc acgataaagt ctttattccc
     8821 cgtctggcgc atatgatgca ccactacggc agtattctgt taccgcatgg cggcagtgcg
     8881 ctggtcgata tgacctacta tgaaaatgcc gtgcacgcaa tgtggctggc aagccaggaa
     8941 gcctgcgata agctaccttc cgggcgtgtg tacaacatca ccaacggcga gcatcgcaca
     9001 ctgcgcagca tcgtgcagaa gctgatcgac gagttgaata ttgactgtcg tattcgttcc
     9061 gtcccctacc cgatgctgga tatgatcgcc cgcagcatgg agcgtttagg ccgcaagtca
     9121 gcaaaagagc cgccgctgac ccactacggc gtctccaagt tgaattttga ctttacgctg
     9181 gatattacgc gtgcgcagga agagttaggt tatcagccgg tcatcaccct ggatgaaggt
     9241 atcgagaaaa ctgccgcctg gctacgcgac cacggaaaac tgccgcgcta atcctgcccg
     9301 tatttctcca gcaatgcttc ggcaatcgcc tgagtttctg catccgcttc gccgttatat
     9361 aacgtcgggc ggaaatgcat ctggaatgcc ataatcacgc gccgctgctc gcgcggtgtc
     9421 atatcaggtt taacgtcata accgtagcgc gccaaaagct ccagcaatga cgcggtatct
     9481 accggtgtgt gcggcgcgcg cccggcaagg taaaagttaa cccgctgcgc atccggccag
     9541 gcaccaatcc cctgctgcgc cagttgctgc cagggaaata atggccccgg atcgtctttg
     9601 cgctgcggtg cgatatccgc atgtgccact acgttttccg gcttgatgtg ataacgggca
     9661 ataatatctt tcgccagtgg aataagcgcc tgaatctgtg ccggttcaaa cggggcaaaa
     9721 tatttcactc cggccgattt ttgccagcca cggttttcca gctcaatgcc aatagaggtg
     9781 tcgttaaggc gcgttgcccc gcgccaggcg ctaatccccg catgccaggc cagttcttgt
     9841 tccggcacca gttgccagat gcgcggttta ccgttgtatc gcggtggtac cgcagggacc
     9901 agataatgcg agctgacctg cttatcggtc agtgtcgcca gcgagctatc aaaatcatct
     9961 gcggtgtagt ggatcaccag cactttaatg cgcggatacg ccgcctgcgc ctggcgtcgg
    10021 gtatcaagct gatatccctc tttctcgaca atgccttttt cgcctgcaca ccctgccaat
    10081 aacagagcgg cagcgaccag ccagaagact cttctcatcg acgcgttttc accgccgtac
    10141 cgctgacgct aaccatcagc atactgccgt tttgcccgac cgtttcgtag tcgatatcaa
    10201 taccgacgac ggcatcggcc cccagcgccc gcgcctggga gcctaattcc tcaaaggcga
    10261 tctcccgtgc tttacgcagt tctttttcat acgcaccgga acgtccgcca acgatatcgc
    10321 ggataccggc aaagaaatca cggaaaatat tggcacctaa aatcgcttcg ccggtcacca
    10381 caccgcaata ttcaacgatg gtctggcctt ccagagttgg ggttgtagaa aattgcatga
    10441 tcgatctcct tgtttgctaa gccttcgatc tcaaaagcat tatcagactg atacgctatt
    10501 attgaaagga tatcattatt atcgtagaac gctttcagag cgatcgctta taaggaaatc
    10561 attatgcgct acagcaaatt gacaatgctt atcccctgcg cactgctcct cagtgcctgc
    10621 accacagtca ctccagctta taaagataac ggcacacgca gtggtccttg cgttgaagga
    10681 ggcccggata acgtggccca gcaattttat gactaccgca ttttgcaccg cagcaacgac
    10741 atcaccgcct tgcgccctta tctgagcgac aaactggcga cactgcttag cgatgcctcc
    10801 cgggataata accatcggga actactgacc aacgatccat tctccagtcg aaccaccttg
    10861 ccagatagcg cccacgttgc cagtgcatcg actatcccta atcgtgatgc ccgtaacatt
    10921 ccattgcgcg tggatctcaa acagggtgac cagggttggc aggatgaagt gttaatgatt
    10981 caggaaggcc agtgttgggt catcgacgat gtgcgttact taggcggtag cgttcatgcc
    11041 acggcgggta cgctccgtca gtccatcgag aatcgctaac gccttgatca accaataaac
    11101 cccgtaaatt gtccggcatt tcgtgaggaa tgccgacatt tatgctcgcc gaccaccgcc
    11161 cccgttattt tgtgctatgt ttattgaata atgcgctttg cttttaactt ttaaagcaga
    11221 aatattgcat aattattctg tcaaaggtac tatcttcgac ctcaatagct atcagactgc
    11281 cagtatacga gtgtcaatga gtattcaatt aaacggcatt aattgcttct acggcgcgca
    11341 tcaggcgctg ttcgatatca cgctggattg cccacagggc gaaacgctgg tgttacttgg
    11401 ccccagcggc gcgggtaaaa gctcgctgct gcgtgtactc aatctgcttg agatgccgcg
    11461 ctccggtacg ctcaacattg caggcaacca tttcgatttc accaaaacac cctctgacaa
    11521 agcgattcgc gatttgcgtc gtaacgttgg catggtgttt cagcaataca acctgtggcc
    11581 gcatctgacc gtgcagcaaa acctgattga agcgccctgc cgtgtactgg ggttgagtaa
    11641 agatcaggcg ctggcccgtg cagaaaaact gctggaacgt ctgcgtctca aaccttatag
    11701 cgatcgttac ccgctgcatc tttctggtgg tcagcagcag cgtgttgcta ttgcccgtgc
    11761 gttgatgatg gaaccgcagg tactgctgtt cgatgaaccg accgccgcac tggacccgga
    11821 aattacggca caaatcgtca gcatcattcg tgagctggca gaaacgaata ttacccaggt
    11881 gatcgtcacc cacgaagttg aagtggcgcg taaaaccgcc agccgagtgg tgtatatgga
    11941 aaatggtcat atcgtagaac aaggcgacgc gagctgcttt accgagccgc aaaccgaagc
    12001 atttaaaaac tatctctctc actaagattc gggataacaa caatgaaaaa agttctgatt
    12061 gccgcgttaa ttgcaggttt tagtctttcc gccacagctg ccgaaaccat tcgttttgct
    12121 accgaagcct cctatcctcc gtttgaatcg attgatgcaa acaaccagat cgttggtttt
    12181 gacgtcgacc tggcacaggc actgtgtaaa gagattgatg caacctgtac tttctctaac
    12241 caggcgtttg acagcctgat cccaagcctg aaattccgtc gcgtagaagc agtgatggcg
    12301 ggcatggata tcactccgga gcgtgaaaag caggtactgt ttaccacccc gtactatgac
    12361 aactctgccc tgtttgtggg tcagcaaggc aaatacacca gtgttgatca gctgaaaggc
    12421 aaaaaagtcg gcgttcagaa cgggacgaca caccagaaat tcattatgga taagcacccg
    12481 gaaatcacta ccgttccgta tgacagctac cagaacgcaa aactggatct gcaaaacggg
    12541 cgtatcgacg gcgtcttcgg tgacaccgca gtggtcactg agtggctgaa agataacccg
    12601 aaactggcgg cggtgggcga caaagtgacc gataaagatt acttcggcac tggcctcggc
    12661 atcgcggtac gtcagggcaa cactgagctg cagcagaaac tcaacactgc gctggaaaaa
    12721 gtgaagaaag atggcactta cgaaaccatc tacaacaaat ggttccagaa gtaattcctg
    12781 atgaatgaat tttttccttt agcaagcgcc gccgggatga ccgtcggcct tgccgtttgt
    12841 gcattgattg tcgggctggc gctggcgatg ttctttgcgg tatgggagtc ggcaaaatgg
    12901 cgtcctgtcg cgtgggcagg ttcagcgctg gtaaccattc tgcgtggcct gccagaaatt
    12961 ctggtggtgc tgtttatcta ttttggctcc tcgcagctgc tgctgacgct ttcggatggc
    13021 ttcactatca atcttgggtt cgtgcagatc ccagtgcaga tggacattga gaacttcgac
    13081 gttagcccgt tcctttgtgg tgtcatcgct ctgtcactgc tgtatgccgc ctatgcctcg
    13141 caaacgcttc ggggcgcgtt gaaagcggtg ccggtgggtc agtgggagtc tggtcaggcg
    13201 ctggggctgt cgaaatcggc tatctttttc cgtctggtga tgccgcagat gtggcgtcat
    13261 gcgctgcctg gcctcggtaa ccagtggctg gtgctgctga aagataccgc gctggtcagt
    13321 ttgattagtg tgaatgattt aatgctgcaa acaaaaagca tcgctactcg tacccaggaa
    13381 ccatttacct ggtacattgt ggcggcggcg atttacctgg tgatcaccct gctcagtcag
    13441 tacattctca aacgcattga cctgcgcgcg acacgttttg agcggaggcc cagctaatgt
    13501 ttgagtattt acccgaactg atgaaagggc tacacaccag cctgacgcta accgttgcct
    13561 cgctgattgt ggcactgatt ctggcattga tttttaccat catcctgacg ctgaaaacgc
    13621 cggtgctggt gtggctggtg cggggttata tcacgctgtt taccggtacg ccgctgctgg
    13681 tgcagatctt cctgatttat tacgggccgg gccagtttcc gactttgcag gagtatccgg
    13741 cactgtggca tttgttgtca gaaccgtggt tatgtgcgct gattgcgtta tcgctgaaca
    13801 gtgcggcgta taccacgcag ctgttttacg gtgcaattcg tgcgatccca gaaggtcagt
    13861 ggcagtcctg tagcgccctg ggaatgagca aaaaagatac gctggcgatc ctgctgccgt
    13921 atgcctttaa acgctcgctc tcttcttatt ccaacgaagt ggtgctggtg ttcaaaagta
    13981 cctctctggc ttacaccatt acgctgatgg aagtgatggg atacagccag ttgttgtacg
    14041 gacgcaccta cgatgtaatg gtgttcggtg cggcagggat tatttacctg gtcgtcaacg
    14101 gcctgctgac gctgatgatg cgtctgatcg agcgcaaagc actggcattc gaacggcgaa
    14161 attaatgtag ctgcgtacat tcgccggggg ggtttgtagg tctgataaga cgcgccagcg
    14221 tcgcatcaga catcagcacg gtgcctgtgc cggatgcggc gtggacgcct tatccggcct
    14281 acatagtgca taaattctta taatgaagac ggacaactca ctaaggtatc cgtctttttt
    14341 tatttcattt aaattattta atcatattta ttgcatataa attcacttga tggcattgtt
    14401 atcccatgcc gcagacacgg ccaaaaatca taagattgac agacgggagt tccatcatga
    14461 aaaagttagt tcttgccgct ttacttgctt cctttacttt cggtgcttct gccgcagaga
    14521 aaatcaattt tggcgtttca gccacctatc caccctttga atctataggt gctaataatg
    14581 agattgtcgg ctttgatatc gatctggcaa aagccttgtg caaacaaatg caggcagaat
    14641 gtacttttac taatcacgcg ttcgacagcc tgatcccgtc actgaaattc agaaaatatg
    14701 acgccgtaat ctccggtatg gatatcacgc ccgagcgtag caagcaggta tcgtttacca
    14761 cgccctacta tgaaaactca gccgtcgtga ttgccaaaaa agatacctac aaaacgtttg
    14821 ccgatctgaa aggcaaacgt attgggatgg aaaacggcac cacgcaccag aaatatattc
    14881 aggatcagca ccccgaagtg aaaactgtct cctatgacag ttatcagaat gcctttatcg
    14941 atctgaaaaa tggtcgtatt gatggggtat ttggtgacac agcggtggta aacgaatggc
    15001 tgaaaaccaa tccgcaactg ggcgttgcta ctgaaaaagt gaccgatccg caatacttcg
    15061 gcaccggtct gggcatcgct gtacgtccgg ataacaaagc tctgctggaa aaactgaata
    15121 acgcgctggc agcaattaaa gctgacggta catatcagaa aatcaatgac cagtggtttc
    15181 cgcagtaatt gagactgatg acaaaagtaa gattgcctga tgcgctgcgc ttatccggcc
    15241 cacatcaact ctgcaatgta ttgatttagc atgattttgt aggccggata aggcgtacac
    15301 gccgcatccg gcatgaacaa agcgcacctg atcagcaatc tgaagccgca cctgcggctt
    15361 ttttattgct tcaccagcag cgtcagcact tcatagtgcg cggtgtgcgg gaacatatca
    15421 aaaagctgta cccgttcgat gcggtaacct ggcagttcgc ggatatcttt cgccatggtt
    15481 tgggcgttac agctggagta gataataaaa cgcggtgcca ttgttgagag ataatcacat
    15541 agcggtttac caatgccgcg gcgcggcgga ttaaccagca ccagctccgg cacctccccc
    15601 tgagcggtgg caaactgagt ggagtccagc gcctgaaatt gcaaacgcgt taagcccagt
    15661 tcagcggctg actgctttgc acaggcaatg gcctctgatg caatttcgat cccggttaac
    15721 tgcatgtcag gcgtcgcgca gtgtaaacca aagccaccca caccgcagaa cagatcccac
    15781 atatgtttaa ctggcagctg ccgtacccag tcgcgcgcgg tagcgtacag ctggctggcg
    15841 accgccggat tagtctggaa gaaactttgc ggacggatcc acagcggtac gtcattaaaa
    15901 cgctctgcca gcgcctgttg ttcagtaagg tagatctccg tctccccttc cataatcgcc
    15961 atatgtaccg gctgaatatt gacggtaata actttcagtt gcggtagttg ttcctgtaac
    16021 cacggcagcg ccttacgcag ttgtgccagt ttggtttcag aacgcagtac aaagcgcagc
    16081 atcatgccgc catcgctctg gctttcagtc agcagaatgt atttcagttc gccacgctta
    16141 cgcgccacgt tgtagggtgt cagccccgca cgggcgataa acggttttag cgccgcaaaa
    16201 acgggcgcaa atgaggcagg ataaagcggg cagtcacaaa ggtcttctgg tgtgccgtcg
    16261 cgatgcagca taccgagcag tggtttttca acgctaccac tcaccaccat tttggcttta
    16321 ttacgaaacc cttgttccgg gcctgacacc ggcgcgcacc attcctcaac cggaaaatcg
    16381 gcgagcagat ttttaagatc ggcggtttta gcggagagtt gctctggaat cggctgcgtt
    16441 atccactgac aggaacgaca gcgacccgcg tcgtaaagtg cgcactgcat acattgacct
    16501 tcacatcatc aggggcgacg attatacaca ttattgcaac tggaagaacc gccgactggt
    16561 cgagggaacg aacagcagca tcaggatgag catatccggc agcttctgca gcatcaggct
    16621 atggaagatt tcacgttttg attcaccggg aatgctgaac agctccggat aaccgtaccc
    16681 cagcgaagcc gcccacaagt aacttgcggc ggtgatttgt gtcagcagat atagccagcg
    16741 cgcccagcga cgccctttca ccagtgaaaa cgcacaccag atctcaatga aaaccagcac
    16801 cagactgctt aaaaagacca gcgttaaact ccaggtttgt acgctgcgat gaatgaactc
    16861 gccaataccg cgcacgccca gcgtattgaa aatcatcagc acgtcgaggc cacggatcat
    16921 aataatggcg agcgccgcca cctgcaccag cgcaggcaca ttcaggcgag catgagatga
    16981 agatgttttc ttaaaaaatc ccaacgtttc gtcttccatg aaaacaatgc cgcggcatgc
    17041 gcggcattat gtagccaggt tggcaaattt tagtgtcttc agccatgtct tgcacgctgg
    17101 atatcgcgta tccgctgttt ttctgcgcga gccatcagat accaggcgat aaatccgaca
    17161 attccgaccg caccgagaat taacgttgca agggcgttga tttccggatt cacccccatc
    17221 cgcacgctgg aaaagaccag catcggtaac gtggtggctc ccggcccgga aacaaagctg
    17281 gcgatcacca gatcatcaag cgacaaagta aaagccagta accagccaga aatgatcgcg
    17341 ggcatgatca tcggtagcgt aatgacgaaa aacactttca gcggcgtcgc accgagatcc
    17401 atcgctgctt cttctatcga gcgatccagt tcccgcaatc ttgaagaaat aacgaccgcc
    17461 acataagccg tacaaaaagt gacatgcgcc agccagatgg tgagcatacc gcggtccgca
    17521 ggccagccaa tggcatgagc aagcgcgacg aataataaca acagcgacaa gcccgtgatg
    17581 acatctggca tcaccagcgg cgcggtgatc ataaaggcaa agccatttga tccgcgaaat
    17641 ctgccaaagc gcaccagcac caccgccgca atagtcccga ggatcgccgc cgccgttgcc
    17701 gcacaggcag caattgtcag gcttaaacca accgcactca tcatcgcgtc atcgcgcaat
    17761 aactcaccat accagcgcgt tgaccagccg gcccacaccg tcaccagctt cgagctgtta
    17821 aacgaataga taaccagcat cagcattggc gcgtagagaa aggtaaagcc cagcagcaaa
    17881 atcacaatcc gccagggcga acgaactacc ggtaaattat tcatccgtgt tctcccacgc
    17941 ttttttgctg gtgtttgtgg aaccacatta tcggcacaat tagcagcagc aacatgatga
    18001 tcgctaccgc cgaggccacc ggccagtcgc ggttattgaa aaactcttgc catagcacgc
    18061 gcccgatcat gatgctgtcc gggccaccga gcagttccgg gatcacaaac tcgcccacag
    18121 ccgggataaa caccagcatc gatccggcaa taatcccacc tttagtcagc ggcacgatca
    18181 cagtaaagaa cgttttcagc ggtcgtgcac cgagatccag cgctgcttcc accagcgaat
    18241 aatcaatacg aatcaacgcg gtataaatcg gcagtaccat aaacggcacg taggcgtaaa
    18301 caatgccaat ataaactgcc agattggtat gaagaatggt cagcggttga tcgataaccc
    18361 ccagccacag cagaaaatta ttcagcacac cgttgttttt taatattccc atccaggcat
    18421 aaacgcggat cagaaacgag gtccacgacg gcaggatcac cagtagtaat aaaatattac
    18481 gggtcgaagg cttactgtgc gccaccgccc acgccagcgg atagccgatc agtaaacagc
    18541 aaaatgtcga aatcgcagcc acctgtagcg actggagata agcatcgaaa tagagcggat
    18601 cgtcggtcag ttgcagaaaa ttaccgagat taagagtgat ggacagttgc ccgtcagccc
    18661 actccatcag ttcggtataa ggtggaatag cgcgcgccat ctccgccagg cttattttaa
    18721 agacaatcag aaatggcagc agaaacagca agatcaacca gatatatggc aacgcaatga
    18781 ccagtttgcg cccatgcttc atttgcagct gcgacaacca cagcttaaat ccgcccggcg
    18841 gtttcgactg ggcagcaggt tcaagtgtat tcatctttgc tccttaaacc gtcagcacca
    18901 cacagctgtc cacttcccag cacaaacgca cttcgtcgcc ccaggtcggt aaccctttac
    18961 gatgacgatg ggcgttttgt agctgggcgc taatcatctg cccacttttc agacgaacgt
    19021 gatacaccga aagatcgccg agataggcaa tgtgtatcac ctcccccacc gcgaagttac
    19081 aaccattggc gggcggctct tcgcaaagca tgattttttc cgggcgcagc gctacgtgta
    19141 ccggcacgtt atcgaccact gaagcatccg catcgacttt cagtggatgc accagccccg
    19201 gcgaatcaag caccaggcca tcttcctgac gctctttgag tacaccttca aagacattaa
    19261 ccgaaccgat aaattcagcg ctgtagcggg tagttggatg ctcgtagatc tcttccggct
    19321 cgccaatctg gacaaatttc ccgcgattca taatggcgat acgccccgcc atggtcatcg
    19381 cctcttcctg atcgtgggtg accatcacac aagtcacacc gacgcgctcc agaatatcca
    19441 ccacttcaag ctgcatccgg tcacgcagct ttttatccag cgcgcccatc ggctcatcga
    19501 gcagtaatag tttcgggcgt ttggcaaggc ttcgggccag ggccacacgc tgtcgctgac
    19561 caccggaaag ctgatgcggt ttgcgtttgg cgaactcctg catgtgcacc aggccgagca
    19621 tctcatggac ccgactggca atttccgctt tcggtagttt gtcctgtttc aggccaaaag
    19681 cgatgttctg ttccacggtc atatgcggaa acagcgcgta agactgaaac atcatattga
    19741 tggggcgcag gtaaggcggc acctgtgaca aatcgacgcc atcaagcatt atctgtccgg
    19801 cagaaggttg ttcgaaacct gccagcatac gcagcagcgt ggacttgcca cagccggatg
    19861 cgcccagcag cgcgaagatt tcacctttgt agatggtcag gctgacatca tccaccgcat
    19921 gttgaccatc gtaggatttg gtgaggttgc ggatttctaa tagcggcgtc agcgccttac
    19981 gggttttcgc ctgcgggcga gggatagcgt cattcacggc tgctctccgg cattgaatgt
    20041 gtgcaagcgc accatgatgg tgcgaattgc cggcgggtgt ggtgcgcccg cccggcatct
    20101 aaggctgcgg attattttcc gctcttcact ttggtccacg cgcgggtgcg cacacggtcg
    20161 attttcgggt cctgcacttt cagagtgaac agcttcgcac gaacatccgc aggcggataa
    20221 atacccgggt tctcacggac ttccgcactc accagcggcg tggctgcttt attggcgttg
    20281 gcatagaaca catggtcgga aatatgcgct actacatccg ggcgcagcag gtaattcagg
    20341 aactgatagg cttcgtcttt gtttttggca tccgcaggca tggcgaatac atcaaagaac
    20401 gccatcgccc cttcttttgg aatcaagaac gagacattaa cgccattctt cgcttccttc
    20461 gcgcggtttg acgcctgcca gacatcgccc gcccagccaa tagcgacgca gatatcgccg
    20521 tttgccaggt cgttaatgta ttgagatgaa tggaaataac gaatgttcgg gcgcagcttt
    20581 aacagcagat cggtagccgg tccggtgtaa tcatccgctt tagtgctgtt gggatctttg
    20641 ccgagataat tcagcacggt agcaaaaact tcttctggcg catccaggaa agagacaccg
    20701 cagcttttca gtttttccag attttcaggc ttgaggatca agtcccagct atcgacgggc
    20761 gcgttttcgc ccagcacagc tttaacttta tcaacgttat agccaatccc ggtcgtcgcc
    20821 cacatatagg gcatagcaaa tttattgtcg ggatcgtgtt tggcgaccaa cttcagcagc
    20881 tccggatcga gattcttcca ctccggcaat ttgcttttgt ccagcggctg gaaaactccc
    20941 gctgtcagct ggcgttcaag aaagctggca gatggaacca ccaagtcaaa gccggtgcta
    21001 cccgccatta atttgccttc cagtacttcg ttagagtcga aaacatcgta gacgacttta
    21061 ataccggttt ctttttcaaa attggccacc gtgtcagggg cgatataatc agaccagtta
    21121 taaatgtgga gtgttttttg ttcagccgcg agcgtgccga cagagacggc catcagagca
    21181 cccgcaacca gacccgatag ccatttttta tttaaggcgg tcatatctct ttccttctga
    21241 aagttcgtta acaaacgaac aaaagttgtc acactgaaag atatgcaata aacgtgcata
    21301 tttttttatc ggcgcggtgt aagaaaagag aactctctcc cggccagcac tgctcgctat
    21361 ataaaagcat cgcaagaata actttagcca gggtttgaga ctatagctcg gattaaaaaa
    21421 cggtatttaa caaattttta tgcgtatttg ctctatgtga taaagccaaa tcgacacaag
    21481 ataagtgaaa taatctttaa gaaaaggtta aaaacagcag ataattttgc agcgcgcagt
    21541 cactgcggca gcgactgcgt gctcgaaagg aattatcagt ggagaaaata gttttcggta
    21601 acgttgtttt gcccttcatc gtcagtcggt aacagcagtt gatgggcatt agcttcaaga
    21661 atgaccatcg aaatctgctc ttcgctctga cgcacgaacc atgcaaactg ctcataggtt
    21721 acgccctgca taacggataa agactggcaa accaccagct ttggcagatt atcatcctgc
    21781 atatcaagaa atgctttcac ggtcagcgaa ctggcattga tggcagataa atccgccgcc
    21841 agcggcaata cggctgaagg tcggacttcc gccatggcag aaaacaggat cgtgttatcg
    21901 atcagatcga ttttggcatc aaagacaccg tcgaaattct gcatatgggg cagatgcaga
    21961 gcctgacagt tatcacattc aaaaaaactc atccccaggt catcgagcca ttgacgcagc
    22021 gtatccagac caggaacgac cagcgatgtc ataattttgt acaacctctt tcgataaaaa
    22081 gaccggcaca gcttacgcaa aaagcgcagg caaaaccatg atcagtaatg tgattgcgac
    22141 taaccacccg ttttcaggca atattctgtc gtagcgtggc gttcgatcca gcggatcatt
    22201 ttacccgcga tgtcgatacc ggtggttttt tctattcctt ccagccccgg cgacgcattc
    22261 acctccatca ccaacggccc gcgattagca cgcagaatat ccacaccagc aacgtccagc
    22321 gccatcgttc gcgcggcttt tatcgcgatt tcacgctcct gtggtgtgat actggcgacg
    22381 cttgccgcgc cgccacgatg caaattggaa cgaaaatcgc cctctttcgc ccgccgttca
    22441 atcgcagcga ccacttcatc gccaacaacc agacagcgga tatcgcaccc ttgcgcctct
    22501 ttgatatatt cctgcaccag aatatgcgcg ttcagaccgc ggaaagcgtc aatcacgctt
    22561 tccgccgcct gacgcgtctc cgccagcacg acgccaattc cctgcgtgcc ttcaaccaac
    22621 ttgaccacca gcggcgcacc accgaccatg tcgattaaat cgctggtatc atccggcgaa
    22681 tgcgcaatgc ccgtgacagg caggtcgatg ccctgacgcg ccagcagttg catggaacgc
    22741 aatttgtcac gcgcccgggc aatggcgacc gactcattga gcggatagct ccccagcatc
    22801 tcgaactggc gcagtgccgc cgtcccataa aaggtaatgg cggtgccaat acgcgggatc
    22861 actgcgtcaa aatggggtaa cttgcggcct ttgtagtgaa tagaagacgc cgcaggattt
    22921 atgttcatgt agcaagaaag cggatcaaga atttcaacca ggtgaccgcg ctgtatagcg
    22981 gcttcacgca gccgcttaca cgaatagagc gttccatccc gggacaatat ggcaattttc
    23041 accctgcacc tctctgtcag acctggtcaa aagcccgcgt atcatacatc gacgtggcgg
    23101 ttttagcgcg tcgcccaacc ctgtttgtgc aaataatcca gaataaatgg gcggctttct
    23161 ttaatgattg ttcggcggat atgatcgctc caggtatccc ggcgattatt gctgccacgg
    23221 gtgaggtaat attccgccag ttgctcgtca tactgcgcca gtgcgccttt atccagcggt
    23281 tgatagctgt tttcatgcac caaaatggag gccggtaaac gcggcttaag atccggatta
    23341 tccgcaggcc agccaaagca cagcccaaac agcggcagaa catgctgcgg taatttaagc
    23401 agtttcgtca ccgcttcaat attattgcgc aggccgccga tatatacccc gcccaatccc
    23461 agcgattccg ctgcgattaa tgcattctgc gccatcattg ccgtatcaac gacaccgagc
    23521 aacagttgtt ccgccaggcc gagctgagca tccggacaga tctgtaaatg gcggttaaag
    23581 tcggcacaga acacccagaa ctccgccgct tgcgctacgt gtttttgccc gccggtcagc
    23641 gtcaccagtt cttcacgtaa cgctttgtcg gtaatgcgaa taatgctact gcactgcaaa
    23701 aaactggaac tggacgtcgc acgggcgctg ttaataatcg cctcacgctg cgcttcggaa
    23761 atgggttcat cagtgaaatg gcgaatggag cgatggccac aaataagttc aatggttggc
    23821 gtcattatct ttttctcttt ctgaacgtga atattgcggt ggacggttca tcagctgtgg
    23881 ggcaagacgt tttgccacct gaagaataac caccaccgca gcgggaagca tgagcaaaac
    23941 accgagaaaa atcatcagaa tctgcacttc tggccgagaa aatggctcag gcagcgacag
    24001 ggagtcgctt accgacagca gcgccaccgc cagtagcatc attccgataa attccagtat
    24061 caacacgcct ttaggcaatt taccgatcgc gcgcatacgc ttccctctgc aaagtgagcc
    24121 ttcagtctaa aacttttcac tgtattgtgt ttaacagtta tagcttttag caattaatgc
    24181 aacaggttaa acctactttc agcgaataca ttttagcgtg atcattacag gcataaatct
    24241 atgaggagag aaataatgca aaccgttatt tttggtcgtt cgggttgccc ttactgtgtg
    24301 cgtgcaaaag atctggctga gaaattgagc aatgaacgcg atgattttca gtatcagtat
    24361 gtagatattc gtgcggaagg gatcactaaa gaagatctac aacaaaaggc aggtaaaccc
    24421 gtagaaaccg tgccgcagat ttttgtcgat cagcaacata tcggcggcta taccgatttt
    24481 gctgcatggg tgaaagaaaa tctggacgcc tgatcgtctg acaagccctc gcgttgaggg
    24541 ctttactgat tttttctgtg ctgtggttta aacaaactac tgataaataa gaaacacagt
    24601 gcccccagcg cacaccagaa caccgcgctt agtaaccatg ccagctcttg ccagaatgag
    24661 cgcgtcggtg aaaaaaacag ccgcataatg agcatcgaac agggtgccgc cagcattgcg
    24721 ccaaacagag gtttcaggac ttctctacgc tgtgaaaaga agctggcgac tgctccagga
    24781 agaatgaaaa atagcaagcc gatttcagga tgcccggcag cccgaaaagc gcctttcatg
    24841 tgcgtcgcca gaaaaaggca caccacaatg aagaggacaa aacagcagat tgcccccgcc
    24901 caacgttgtt tatgtttcac tcgttcctcc tgacactgcg tctatcgaac acatttttcg
    24961 ccagtgtggc gttcagtaag ataaagccgc ttcgcattcc atgctaatat aggccaacgc
    25021 aattcatata gccgttgata cctaatgtga ttacactagt aaaatatatt gttactttac
    25081 tatcgtttag gtgcgctgaa tgaatctgcg ccctgaattc tggtaaaaaa cattatcgta
    25141 aattaccatt tctttcaaca gcttactagt aaacaagaag ttagcctccg tgaatataaa
    25201 cgtcgccgaa ttgttaaatg ggaattacat tctgttatta tttgtggtcc tcgcgcttgg
    25261 gctatgtctc ggaaagttac gacttggttc gatccaactg ggtaattcca ttggcgtttt
    25321 agtcgtatcg ctgttattag gccaacaaca tttcagcatt aacaccgatg cgcttaatct
    25381 tggctttatg ctgtttattt tctgcgtcgg ggtcgaagcc ggaccgaact ttttttccat
    25441 tttttttcgc gatgggaaaa attacctaat gttagcactg gtgatggttg gcagtgcgct
    25501 ggtgatcgcc ttagggttag gtaagctgtt tggctgggat attggcctga cggccggtat
    25561 gttagcaggc tctatgacgt cgacaccggt tctggtcggt gctggcgata cactgcgtca
    25621 ttccggcatg gaaagcaggc agctctcact ggcactggat aatctgagcc tcgggtatgc
    25681 cttaacctat ttaatcggtc tggtgagttt gattgttggt gcgcgttact tgccgaaatt
    25741 gcagcatcag gacttacaga ccagcgccca gcaaatcgcc cgcgaacgtg gcctggacac
    25801 tgatgccaac cgtaaggttt atttaccggt gatccgcgcc tatcgcgtcg gcccggagct
    25861 ggtggcctgg accgacggca aaaatctgcg tgaactgggt atttatcgac aaaccggctg
    25921 ctacattgaa cgtattcgac gtaacgggat tctggcaaat ccagacggtg atgccgtgct
    25981 acaaatgggc gatgaaatag cgttggtagg ctatcccgac gcccatgccc gactcgatcc
    26041 cagcttccgt aacggtaaag aagttttcga tcgtgacctt ctcgacatgc gtatcgtcac
    26101 tgaagaagtg gtcgttaaaa accataacgc tgtaggtaaa cgtctcgcac aactgaagtt
    26161 gaccgatcac ggttgcttcc ttaaccgcgt cattcgtagc cagattgaga tgccgataga
    26221 tgacaacgtc gtgcttaaca aaggtgacgt tttacaagtc agcggtgatg cccgtcgcgt
    26281 aaaaaccatc gccgatcgca tcggctttat ctcgattcac agccaggtca ctgacctgct
    26341 ggcattttgc gccttctttg ttattgggct gatgatcggg atgatcacct tccagttcag
    26401 cacattcagt ttcggcatgg ggaacgctgc cgggttgtta ttcgccggaa ttatgctggg
    26461 ctttatgcgt gctaaccacc cgaccttcgg ttacattccg caaggtgcat taagcatggt
    26521 gaaagagttc ggcttgatgg tgtttatggc aggcgttggt ctgagcgccg gtagcggtat
    26581 taataacggc ctgggcgcga ttggcggtca gatgttgatt gccggattaa ttgtcagtct
    26641 tgtgcccgtg gttatctgtt tcttgttcgg tgcttatgta ttgcgaatga accgcgcact
    26701 gttgttcggc gcaatgatgg gcgcacgcac ctgcgcgccg gcaatggaga tcatcagtga
    26761 tacagctcgc agtaacatcc ctgcgctggg ctacgcgggc acctacgcaa tcgccaacgt
    26821 cctgctgacg ctggcaggga caatcatcgt catggtatgg ccaggattag gataaaactg
    26881 aagttgccct gaaaatgaaa tttttttgca caaccgcaga acttttccgc agggcatcag
    26941 tcttaattag tgccactgct tttctttgat gtccccattt tgtggagccc atcaaccccg
    27001 ccatttcggt tcaaggttga tgggtttttt gttatctaaa acttatctac taccctctca
    27061 accatcctca aaatctcctc gcgcgatagc ggcttacggt cggtgacaaa atgcagcgtc
    27121 atcccctcaa taaacgcatc aagcgcgcgg gcggttccgg gttcaaacca ttgttcgagc
    27181 gtttgctgac tgcgctgcat ccagttttgc attaccgttt ttaatagcgg ttttcggcta
    27241 gccagcgcgt agagctggta catcagctcc atattatccg gcgttgcaac ctgtgagctg
    27301 tagatcatat cggtgatagc ctggcatgcg ccttgagcat cactaacatc gctaaaaaat
    27361 gcctgatatt gccgggacat aatctcagta aaactgctga acgcctccag caacaactca
    27421 tcaattcctg aaaaatagta ggtcatcgat cccaacggta ccccggcaag ggtagcaatt
    27481 ttgcggtgcg taacagcatg tattccgtaa agtttcaccg cctccagcgt ggcctggata
    27541 attttttctc gccgttgcgg atcgttagcg cgacgcatgt catttcctct ctctgcaatt
    27601 gtgtacaaat gtacacaacc ttgctaatgt tgtcttccct cttcttattt tgacgtgatc
    27661 tatgaccgta aattcttcac gtaatgcatt gaaacgccga acctgggcgc tgtttatgtt
    27721 cttctttttg ccaggcctgt taatggcgtc ctgggcaacc cgtacgcctg ctatccgcga
    27781 tattctctct gtctcgatcg ctgaaatggg cggtgttctc tttggtctgt cgatcggttc
    27841 gatgagcggt attctctgct cggcgtggtt agtgaaacgc tttggcacgc gtaatgtcat
    27901 cctggtcacg atgtcctgcg cattgatcgg gatgatgata ttaagtctgg cactctggct
    27961 gacatcgccc ctgctctttg ccgttggtct cggcgtcttt ggggcaagtt ttggttctgc
    28021 ggaagtggcg ataaacgttg aaggtgccgc cgttgagcga gaaatgaata aaacggtttt
    28081 gccaatgatg cacggttttt atagcctggg cacgctggca ggcgctggtg tcgggatggc
    28141 actgacggcc tttggcgttc cggcaacggt gcacatttta ttggcggcgc tggtaggtat
    28201 cgcgcctatt tatatcgcca ttcaggcaat ccctgacggt acgggcaaaa atgctgccga
    28261 tggcacccag catggcgaaa aaggcattcc tttttatcgc gatatccagt tgctgctgat
    28321 aggtgttgtg gtgctggcga tggcctttgc cgaaggttct gccaacgact ggttaccctt
    28381 attaatggtt gacggtcacg gttttagccc tacttccggc tcgctgattt atgccggttt
    28441 taccctgggg atgaccgttg gacgcttcac cggcggttgg ttcatcgacc gttacagtcg
    28501 cgttgccgtg gttcgggcca gtgcactaat gggggcgttg ggtattgggc tgattatttt
    28561 tgtcgatagc gcctgggtcg ctggggtgtc tgttgtactc tggggactgg gtgcctcgct
    28621 gggcttcccg ctgaccattt ctgccgccag cgataccggc cccgatgcac caacccgcgt
    28681 cagtgtggta gcaacgaccg gttatctggc tttcctcgtc gggccgccgc tgctgggcta
    28741 tctcggcgaa cattatggat tacgtagtgc aatgctggtt gtactggcgc tggttattct
    28801 cgcggctatt gtcgcgaaag ccgtcgccaa acccgatacc aaaacgcaga cggcgatgga
    28861 gaatagttga tgagcattaa attaattgcg gtagacatgg atggtacttt cttaagcgat
    28921 caaaaaacct ataaccgtga gcggtttatg gctcagtatc agcaaatgaa agcacaagga
    28981 attcgctttg tagtcgccag cgggaatcaa tattatcagt tgatctcttt cttccctgaa
    29041 attgcgaatg aaattgcctt tgtggctgaa aacggcggct gggtagtgag cgaaggcaaa
    29101 gatgttttta atggcgagct gtcgaaggat gcgtttacta ctgtcgtgga acatttgctg
    29161 acgcgcccgg aagtggaaat tattgcctgc ggaaaaaata gcgcctatac gctcaaaaaa
    29221 tatgacgatg ccatgaaaac ggtggcggaa atgtattatc accgtctgga atacgtcgat
    29281 aactttgaca acttagagga tatcttcttt aagtttggcc tgaatctttc cgatgaactg
    29341 attccacaag tacaaaaagc attacatgag gccatcggcg atattatggt gccggtccac
    29401 accggcaacg gcagcatcga tctgattatc cccggcgtac ataaagccaa tggccttcgc
    29461 caactgcaga aattatgggg aatagacgac agcgaagtgg tggtctttgg cgatggcggt
    29521 aacgatattg agatgctgcg tcaggcaggc tttagttttg caatggaaaa tgccggcaat
    29581 gcggtcgtcg cagcggcaaa ataccgggca ggctccaata accgtgaagg cgtactggat
    29641 gtgatcgata aagtgcttaa acacgaagcg ccatttgacc aataaaaacg gcagcacgcc
    29701 cggcaacaca atacctgaat gaaggctgca taattcgcgg aaattaaccc gctgcattat
    29761 ttcaggcgaa atgtttatga aaaattgtct actgttgggc gcgcttttaa tgggctttac
    29821 tggtgtggcg atggcgcaaa gtgtcaccgt cgatgtgccg agtggataca aagtggttgt
    29881 ggttcctgat tcagtcagcg ttccacaagc ggtcagcgtc gcgactgtgc cgcaaaccgt
    29941 ctatgtggct cctgcgccag ctccggctta ccgcccgcat ccttatgttc gccatcttgc
    30001 cagcgtaggt gaagggatgg ttatcgaaca ccagattgac gaccatcacc attaattcga
    30061 gaatgcctga tcgcacaaag caatcaggca tttttttacc cttcgtgaga atttcccatc
    30121 tgtttatctt ttaaaaagat aaccatcagc gacagccaca aaattccgtt gacaaggttg
    30181 aagagattaa acagtccgtt gcccccgttc agccaggcat gtttgctgat ttcaataccg
    30241 acggtaaaga tcagcatttg cagcattccc atcgccgcag aaaccgtacc tttactcata
    30301 tcgctggcaa acagggttaa tcgcaccagt cccgcattcg ccagaccaat gccgaaagca
    30361 taaatactta acccggcggt catccataaa tacgcgtgcg atgagataac cgttgccgca
    30421 gcagcgacca atagaccaat cataatcggc cagccgccca taataatcag cgaacgtacg
    30481 gtgcggcgcg aggtcagacg cgctaacagc aagttacccg caattaacgc cccgaaaata
    30541 ggcacttgca gcaagccata ttcatagctg ctcaactgct cgccggtaat gatgataatc
    30601 ggcgactggg cgatccacgc cagcaacggc agactaacga atcccagcgc cagcgccccc
    30661 gccacaaagc ggccgttctt cagcaccagc ttatagtcac gaccgagttc tttcagtgac
    30721 agtttctcgc ctatacgcgt ggcggtttca ggcatggctc gttgcagacc gaaaaaggag
    30781 atcgctgcca atgcggcaaa caaaacaaac atcccctccc agggcagcac atggatccac
    30841 gccgcgccca ccagcggacc aagtagcgga gcaatcagcg ccacgttcgc catcagcgcg
    30901 gtgatcttga tacaaaccgc ctcttcgaag gattcctgaa ttgcggcgta tcccacagcg
    30961 ccaatgaaac agaggcttat gccctgcaag aagcgcaaca gggtgaattg ttgtatgttt
    31021 tgcgccagta atattgccag acaggtgacg ataaaccaca ccacacccgc cagcatcacc
    31081 ggacggcgac caatacgatc cgacagcggc cccaggagcc attgtaaaaa catcccgcct
    31141 gccagatacg cggtcatcga agtaggaacc caatcaatgc ccgcctgata ttgttccacc
    31201 acggccaaca taccgggttg aatcatatcg ttgccgatat aggttgaaaa ttcgtaaagc
    31261 accagacaga gagggaaaag taacgcctga cgtccaagcc tggcaccgga agctaattta
    31321 ttttgcatgc aatttcttcg ccaataataa tcgcgcagag tttaataaaa gcgcagctaa
    31381 cgagaaagcg aattttgtag ctgaaaccac ggttaagcac attcttacat tattgcgagt
    31441 atagctacgc tttctttaag ttttatttaa cctatgcccg ttacaatcac ccaccgtaaa
    31501 caggccgctt gagggaaata agacgatgcc gctttaccca gtttaacctg cactttattc
    31561 tcaacgactt gcctgtattg gctccctttt aatcactttg cgtcgggaag ttatgctgga
    31621 aaatttgaat ctctctctat tctctcttat taacgcgacg ccagactcgg ctccgtggat
    31681 gatctcgttg gcgattttta ttgctaaaga tttgattacc gtggtgccgc tgctggccgc
    31741 ggtactttgg ttgtgggggc ttacagcaca acggcaactg gtgataaaaa tcgctatcgc
    31801 gctggcggtc agcctatttg tgtcctggac gatgggacat ctttttccgc acgaccgacc
    31861 ctttgtcgaa aatatcggct ataacttcct gcatcatgcg gcggatgact cattcccaag
    31921 cgatcacggt acggtgattt tcacctttgc actggcattt ttatgctggc atcgcctgtg
    31981 gtccggctca cttttaatgg tgctggccgt catcattgcc tggtcgcgcg tttatcttgg
    32041 cgtccactgg ccgctggata tgctcggtgg attgctggca ggtatgattg gctgccttag
    32101 tgcccagatt atctggcaag cgatggggca taaactctat caacgtctgc aatcgtggta
    32161 tcgcttctgt tttgcattac cgatccgcaa aggctgggtg cgtgactgat ctctgctaaa
    32221 aagtgtagta ttgagcggct cgcttcaata actattcaga gggattatgg aaacacgtcg
    32281 cgaagagcgt atcgggcagc tgctgcaaga attaaaacgc agcgataagt tacatcttaa
    32341 agacgccgcc gccctgcttg gggtttcgga gatgacgatt cgtcgcgatc tgaacaacca
    32401 cagtgcgccc gtcgttttgc tcggcggcta tattgttctg gaaccgcgca gtgccagcca
    32461 ttatctgtta agcgatcaaa aatcccgcct ggtggaagaa aaacgccggg cggcaaaact
    32521 ggctgcgacg ctggtagaac ccgatcagac cctctttttt gactgtggca ccaccacgcc
    32581 gtggattatt gaagcgattg ataatgaaat acctttcacc gccgtttgtt attcgctaaa
    32641 tacctttctg gcgctgaaag agaaacccca ttgccgcgca tttctgtgcg gtggtgaatt
    32701 tcacgccagc aacgccattt tcaaacccat cgattttcag caaacgctga ataatttttg
    32761 cccggatatc gctttttatt ctgcggcggg cgtgcatgtc agtaaaggcg ctacctgttt
    32821 taatcttgaa gagttgccgg taaaacactg ggccatgtcg atggcgcaaa agcatgtgct
    32881 ggttgtcgac cacagtaaat ttggcaaggt gcgtccggcg cgcatgggtg acctgaaacg
    32941 ctttgatatt gtggtgagcg attgttgccc ggaagatgag tatgtgaagt acgcgcagac
    33001 gcagcgcatt aagttgatgt attaatgacg tataaccgga tgacgtttcg cgccatccgg
    33061 ttatcagaag attaagagaa ccagctgccg aaccactgat ggaatttcat catcacgaaa
    33121 tcccacatcc gaccaaagaa tccgccctct tccacatttt ccatcacgat cagcggacgc
    33181 tgctcaatgg atttaccgtt aagctggaaa tcaatggtcc cgacaacctg accttttttc
    33241 agcggtgcgg taagctgcgg ttccgttaac gtataactcg ctttcaggtt tttcagctgc
    33301 ccacgcggta tggtcactga gcccgcttca cctgccccga gattcacttc gctcttatca
    33361 ccaaaccaga cgcgctgagt cacaaaggtg gcatcaggtt taattggcgt cacggtttca
    33421 aagaagcgga aaccccaggt caataatttc tcagactcat taaaacggat acggtcggtt
    33481 ttcgccccca acactacgga gattaaacgc atatcgccct gggtagccga agcaaccaga
    33541 ttatatcccg cgcctgccgt ggttcctgtc ttcatgccat caacattcag attgctgctc
    33601 cacagcagac ggttacggtt aggctgacga attttgttga aggtgaactc tttctcttta
    33661 tgaatggcgt actcttccgg cacatcgtgg atcaatgctt tacccagcaa tgccatatcg
    33721 cgcgcggtgc tgaactgccc cggcgcatcc aggccgtgca ccgtctggaa ggtagtgttg
    33781 gtcagaccca gttttttggc ataaccattc atcagaccaa taaatgactc ctggctcccg
    33841 gcaacgtaat cagccagcgc aatacaggcg tcattaccgg actggataat cacacctttg
    33901 ttcaagtctg ccaccgaaac ctgatcgccc ggtttgagga acattaccga tgaaccacgc
    33961 agtgccggat ttcccgtcgc ccaggcatct ttaccgaccg tcaccatatc ggtgagttta
    34021 atcttatcgg ccttaagcgc ctgcccaacc acatagctgg tcatgatttt agtcaggctc
    34081 gcgggatcca gtttctcatc cgcgttgcct tctgccagca ctttaccgct ggcgtaatcc
    34141 attaaaatcc atgcacgcgc atccacgctc ggcgcttcaa cggtttgttc cgccgcgaat
    34201 gccgttgggg caaaaaggaa taaaaatgca gaacccgctg caagaccacg aaggagagag
    34261 gagtattgcg tcataaatgc cacccgagta tccattcaaa aaaaatacgt tatatcgccg
    34321 tatgataagt tcggtgatta ataacgcaca cacaaactta aagaaaccgt aagttggtaa
    34381 agtttttaaa gtttatgcaa tatcctgccg cttcgctgct tctggtgtca tttttttgcc
    34441 agctgttgct taaaagttaa ctttacttca ccatcggagt cctgttcagc ctgtggctgc
    34501 ctgtcggaaa aggggtgagt atgattacgc tgtggggtcg gaataattca accaacgtaa
    34561 aaaaagtatt gctgacgctc gaagaactgg aactacctta tgagcaaatt ctcgcgggcc
    34621 gtgagtttgg gataaatcac gatgctgatt ttctggcgat gaaccctaac gggctggtgc
    34681 cgttgttgcg tgacgacgaa agtgatctca ttctttggga atcaaacgcc attgtccgct
    34741 atctggcggc acagtacggg caaaaacgtc tgtggatcga ctcaccggca cgtcgtgcgg
    34801 aagcggaaaa atggatggac tgggcaaacc agacgctcag taatgctcat cgcgggatcc
    34861 tgatgggatt agtcagaaca ccaccggaag agcgcgatca ggccgccatt gatgccagtt
    34921 gcaaagagtg cgacgccctg tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt
    34981 tctctggcga cgagtttggt gtgggcgata tcgctatcgc accgttcatc tacaatttgt
    35041 ttaacgttgg cctgacctgg acaccgcgtc caaatctgca acgctggtat cagcaactca
    35101 ctgaacgccc cgcagtgcgc aaagtggtga tgattcccgt tagctaattg cgtgggctaa
    35161 ctttaagtaa ttccccactg gactcgtcgg tgagaacgta taaataaccg tcgggtccag
    35221 tgcgaacatc acgaattcgc tgccctctgt ccgttaaaat acggccatct tctgtcactt
    35281 tgtcgccgtt gacgctcatc acaatgacat ctttatcttt cagcgcgcca ataaataatt
    35341 tttgctgcca ctgggggaat ttatcgctgt tatagaaggc catgccgctc acagcgggcg
    35401 aatctttcca gtaaaaaaca ggttgctcgg tcccggcgac gatctcccct ttcgcttccg
    35461 gtatcttaaa gcctgaatag ttgattcccc aggttgccag cggccagccg tagtttttgc
    35521 ctttttgcgg gatattaatt tcatcaccac cgcgcgggcc atgttcattc agccacagtg
    35581 cattactcca cggattcatc gccattcctt gcggattacg aatgccataa gaccagatct
    35641 cggcgcgcac accggattcc tttataaaag gattatcatc cgggatttcg ccctggtcgg
    35701 tcagacgcac cagtttgccc tgtaatttat ccagatcctg cgccgtcggg cgctgattgt
    35761 tttcgcccag agcaataaaa agataacctt taccgtcgaa taccagccgc ccgccaaaat
    35821 ggttgccggt agacagtttt ggcatctggc ggaagacggt gcggaagtcg gtcacttttg
    35881 agagatcatc acttaagcgg ccataaccca cagcagttcc ggctttgcca tcatcgccaa
    35941 cttcggaata acttaaccag atgcggcgag actgagcaaa atcaggcgct aaaaccacgt
    36001 ccagcaggcc gccctgcccg tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata
    36061 atccttttcc tgcttgccag tgacgcaact cgccgccgcg cagagtgatt aacataccgt
    36121 gattatcggg taaaaaggcc agtgcccagg gatggtcgag tttgtcttgc agtacttcga
    36181 catttaccgt tgcaggagcc gcccagagag cggaagaaag acaaataagg ggcacaagga
    36241 aaaaggattg tcgatgcata acgcctccgg attgactctg gcttaaagcg tagtcagtgg
    36301 aggagataac aaattcattt ttacaaaaac ttaaacatga agggggagac gctttctccc
    36361 ccttagtttt caggccttct caagcatggc gtgcttctgc aggctctgga tactcagcgt
    36421 taagctcatc agacaatttt caagcttatc ggcgttgacg gtaataacag tcgggcaatc
    36481 atggtgccca ctcatcaaac atactgcggc tgtcgctaat gcttcttcag catgatgaag
    36541 agcactccac tcttcctgat ccagatgaag attcaaccgc agcgatttat cgtgcagttc
    36601 gcgattcagt ttaaaaaagt tatctcgtag atgattgctt tcgctgacgg acatgtatcc
    36661 ttttgccttc ctcagttgca cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt
    36721 cagcagatcg attcgctgtc tgtcaacgaa catacttcga tcctcctctt tccagccttg
    36781 ttgcaattaa atagtatggc tattttttga gcaaaatgct gtccaggatc aattcttcac
    36841 gccgaattaa caaaatttac atttccttcg cctccgccag cgaatgagat tgcgatacaa
    36901 attttatcca gaactctgta gaatccctgc cctgaccttt cctgtaactg aatatttttt
    36961 aagctatgag caaagtaact ccccagccga aaatcggctt tgtttccctt ggctgtccga
    37021 aaaaccttgt cgattcagag cgtattctca ccgaactccg cactgaaggt tatgacgtgg
    37081 taccgagcta tgacgatgcg gacatggtga tcgtcaacac ctgcggcttt attgacagcg
    37141 cggtacaaga atcactggaa gccattggtg aagcgttgaa tgaaaacggc aaggtaattg
    37201 tgaccggttg tctgggggca aaagaagatc agatccgcga agtccacccg aaagtgctgg
    37261 aaattaccgg gcctcatagc tatgagcagg ttctggagca cgttcatcac tacgtgccaa
    37321 aaccgaaaca caacccattc ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc
    37381 gtcattatgc ctatctgaaa atttcggaag gctgtaatca ccgctgcacc ttctgcatta
    37441 ttccgtctat gcgcggcgac ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga
    37501 aacgtctggt agatgcgggc gttaaagaga ttctggtgat ctcgcaggat acttccgcct
    37561 atggcgttga tgttaaacat cgtactggct tccacaacgg cgagccggta aaaaccagca
    37621 tggtcagcct gtgcgaacag ttatcgaaac tggggatctg gacacgtctg cactacgttt
    37681 acccttatcc gcatgtggac gacgtcatcc cactgatggc agaaggcaaa atcctgccgt
    37741 atctggacat tccgttgcag cacgccagcc cgcgcattct caaactgatg aagcgtccgg
    37801 gttctgtaga tcgccaactg gcgcgcatca aacagtggcg cgaaatctgc ccggaactga
    37861 ccctacgctc aacctttatt gtcggcttcc ctggcgagac agaagaagat ttccagatgc
    37921 tactcgactt cctgaaagaa gcgcgtctgg atcgcgttgg ctgctttaaa tacagcccgg
    37981 ttgaaggtgc agacgccaat gccctgcctg accaggttcc ggaagaagtt aaagaagaac
    38041 gctggaaccg tttcatgcag ctgcagcagc agatttccgc cgagcgcctg caagagaaag
    38101 tgggccgtga aattctggtg attatcgacg aagtggacga agaaggcgcg attggtcgca
    38161 gcatggcaga tgcaccggaa atcgacggcg cggtttatct caatggtgaa accaacgtta
    38221 agccgggtga tatcctgcgt gtgaaagtcg agcacgccga tgagtacgat ttgtggggta
    38281 gccgggttta attaattcag gccgggcaaa atcatttgtc cggcctgcga ataatgatta
    38341 ctgccagcta atctgagccc tgccgttttt cttactctta tacagccgct catccgccag
    38401 cattttcaag cgttcgacgg tggtacattc atttgaatgc gctacaccga tactggtact
    38461 taaattaatg cttcccacct ggaaatgaac gatttctttt agaatcgata gcaactcttc
    38521 cagcgttcca cccgtataaa tcaccgcaaa ttcgtcaccg ccgaagcggt agattttacc
    38581 tttatctacc gggatctgtg atttaaggat tttcaccact ttcatcaaca cttcatcgcc
    38641 cagcaaatga ccgaaggtgt cgttaatgtt tttgaattta tcgacgtcaa acaggcacaa
    38701 ataaccgcct ttttccggca gcgcttttaa atcttcgtca aaggattttc tgcccattgc
    38761 tttggtcagg tcgtccagga acgccgagtt atacatactg tagagttttt tgacatcggc
    38821 agccgaaaag aaaagcccgc catccagtac gcgttcaaaa tggttattaa tgatggcttc
    38881 tttctccaca ataccacgat taatcactct gcgtaccaga tagaggcaac tggtacccga
    38941 tatcactgcc accaatgcgg caaagaaaat aatcaatgta cagacccgca aataaacatc
    39001 ttttttatac gcaaatacgt acaatccggt cggcaaatag gcgaagtgct ccatcgcttc
    39061 cgaaacggtg aagatggcgt ttttagcata atattcatca ccggtcaccg gtttaccgtt
    39121 cttaaataga gagaacatca ggttctcgct gtccatcgcc agcgattttg acagtaaaga
    39181 aaaacgctcc agaccaagca aaaatatcag actacgattg tgagttaccg gatagaggta
    39241 atagacgaag gttttaccgt caaaagagat aaaccctgta tttttgacat gtcgatactt
    39301 cacaacctgc ataatatctg cacaataatt tttctcgaac tcctttacct gaatacattc
    39361 gtcactggaa atcagataag gaccgacatc gtacaagtaa gagggtgaaa ccgagtcagc
    39421 gtaagctgtg cgtaagccaa ccaacagctg ttcctccgtg ggatttagcg tattgataag
    39481 atcaattgcc acgctacgcc gatattcagc aatatttgcc accgcaaaag cagtatcaat
    39541 aacggactgt ttcaggctat acacccgttc ctttaccatt tgagtgtaga tcccgcaggc
    39601 aactgctgaa atcatgataa aaatcagcag actgactgta agtacgaact tattgattct
    39661 ggacatacgt aaactactct tttactaatt ttccactttt atcccaggcg gagaatggca
    39721 tgggtttctg gtacagatac ccttgggcct gaacacatcc taatttgatt aattcgcgcg
    39781 caacgtcttc gctctctacg ccttccgcaa tcaccgggat ccggttatag cgtgccagat
    39841 cgaaaataat gcgcacaatt tttcccgcag taccgaaaat atcgccggta aattgcgagt
    39901 ccacctttat gctgtctgga ttaatttcat aaaaacgttt caggtttgac aaaccggtgc
    39961 caaagtcatc aatcgatatc cgtacccctt gtttacgcag ttgttcaata ttgagataaa
    40021 aatgttcatt aattcgctcg atcacggttt cagtgatctc aacacagagg ctgttcgcct
    40081 tcaggccatt ttctttcatg atggtggtaa aacgctcatg aaagtgcggt tcgtttaact
    40141 gccagtgcga aacattaata tgcagtaaga aatcatcctt cagcgacgca cgctgactaa
    40201 aatgtctgaa ttcacccatt gccgttttaa taatctgata acccagctca ttgataaacc
    40261 cgatatcttc agcaatggta atgaacttca gcggtgaaat gatcccccgc tccgcagaaa
    40321 cccaacggca caacgcctcc gcgcccagaa tggcgcgggt gttaatatca acgatcggtt
    40381 gatagacgag gaatagttcg ccaatttcca ttgcatggcg aatattacgc gcaatccaga
    40441 tattctcttc tatttcgttg agtttcgcgt catcacaaaa aataaactcg gagaacatat
    40501 ggtcacggat actggaaatc gccagtttca gtttttctgc gtaactccag gcgttttccc
    40561 cctgcaaagg ttcacaaatg gcagacttat ggatgcgcag attgcggtag ccaaagctgc
    40621 tgtcaaaatc gtttaccatc gctattttac gggtaaatgt ctgtacattt tcccgacaca
    40681 gaagtaaata gagatcggca ttgaatgtca cgatatcgca gcattgcgca tatttctcgg
    40741 caattcgctg acacaatttc ctgacgattt tattgcgttc atcaacacca aacaccgctt
    40801 ccacggcttc cagaccacac agtttgatga tccccacagc cagatactga ttattgttgg
    40861 actcaataat cgccatattt ttaataaaac cgctttcctg atcgtaagag atcagtgaga
    40921 gcaatttatt tgaccgttca atttcggcat ctttcatttc cgcgatcgaa tttttgatgc
    40981 tgataatctc tttcgcatcg ccttcgcgaa ttttcagagc ctcgatttga tccgttgagt
    41041 cgggcaaaaa cgccagcgta ttaaccagct cggtcatttc agaaaattgc cgacggaaca
    41101 cacgagtggt catgaaatat accaccagca acacgataat aatgaacgca aacggtagat
    41161 aaaaaagttt atctgatgcg ttcaataaat taccgccagg ataataaaaa tagatttcga
    41221 acaatgcatt atttggcact
//
LOCUS       DAZFRN010000027        40553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFRN010000027 DAZFRN010000000
VERSION     DAZFRN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.027"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            100..279
                     /locus_tag="ACHKUB_001373"
     CDS             100..279
                     /locus_tag="ACHKUB_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011816234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654412.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKE"
     gene            337..540
                     /locus_tag="ACHKUB_001374"
     CDS             337..540
                     /locus_tag="ACHKUB_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654413.1"
                     /translation="MTNKESTNTPSQKTNREHENMALEHESERFAPCAFVLDEFLKKY
                     SRSERMKMAAQYGPNKQGNCPPA"
     gene            complement(784..972)
                     /locus_tag="ACHKUB_001375"
     CDS             complement(784..972)
                     /locus_tag="ACHKUB_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6654414.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(1337..2566)
                     /locus_tag="ACHKUB_001376"
     CDS             complement(1337..2566)
                     /locus_tag="ACHKUB_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6654415.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHEMNYAVQE
                     ALKPVTVGDALTYWLESYVKENRVDYIALKRRLNNHVIQYIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQTCKQALKFCRRRRFAISNVLDDLNVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTRLVDQNRHTGLLLGEVKQEASVSQYGRLAHRRLKHPQWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYIDAKRNALDMWVERLEI
                     LAGTHENVTTLPVTRIK"
     gene            2554..2736
                     /locus_tag="ACHKUB_001377"
     CDS             2554..2736
                     /locus_tag="ACHKUB_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654416.1"
                     /translation="MLCSRFTPCLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMNNLKHYETAKNTN"
     gene            2743..3648
                     /gene="tqsA"
                     /locus_tag="ACHKUB_001378"
                     /pseudo
     CDS             2743..3648
                     /gene="tqsA"
                     /locus_tag="ACHKUB_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(3673..5061)
                     /gene="pntB"
                     /locus_tag="ACHKUB_001379"
     CDS             complement(3673..5061)
                     /gene="pntB"
                     /locus_tag="ACHKUB_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO6654417.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5072..6604)
                     /gene="pntA"
                     /locus_tag="ACHKUB_001380"
     CDS             complement(5072..6604)
                     /gene="pntA"
                     /locus_tag="ACHKUB_001380"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO6654418.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7128..8072
                     /gene="ydgH"
                     /locus_tag="ACHKUB_001381"
     CDS             7128..8072
                     /gene="ydgH"
                     /locus_tag="ACHKUB_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO6654419.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8258..9640
                     /gene="ydgI"
                     /locus_tag="ACHKUB_001382"
     CDS             8258..9640
                     /gene="ydgI"
                     /locus_tag="ACHKUB_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6654420.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9677..10399
                     /gene="folM"
                     /locus_tag="ACHKUB_001383"
     CDS             9677..10399
                     /gene="folM"
                     /locus_tag="ACHKUB_001383"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO6654421.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10396..10731)
                     /gene="ydgC"
                     /locus_tag="ACHKUB_001384"
     CDS             complement(10396..10731)
                     /gene="ydgC"
                     /locus_tag="ACHKUB_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO6654422.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            10860..11579
                     /gene="rstA"
                     /locus_tag="ACHKUB_001385"
     CDS             10860..11579
                     /gene="rstA"
                     /locus_tag="ACHKUB_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO6654423.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKDLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11583..12884
                     /gene="rstB"
                     /locus_tag="ACHKUB_001386"
     CDS             11583..12884
                     /gene="rstB"
                     /locus_tag="ACHKUB_001386"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO6654424.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12960..13889
                     /gene="tus"
                     /locus_tag="ACHKUB_001387"
     CDS             12960..13889
                     /gene="tus"
                     /locus_tag="ACHKUB_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO6654425.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVSE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13886..15289)
                     /gene="fumC"
                     /locus_tag="ACHKUB_001388"
     CDS             complement(13886..15289)
                     /gene="fumC"
                     /locus_tag="ACHKUB_001388"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO6654426.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15432..17078)
                     /gene="fumB"
                     /locus_tag="ACHKUB_001389"
     CDS             complement(15432..17078)
                     /gene="fumB"
                     /locus_tag="ACHKUB_001389"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6654427.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17277..18452
                     /gene="manA"
                     /locus_tag="ACHKUB_001390"
     CDS             17277..18452
                     /gene="manA"
                     /locus_tag="ACHKUB_001390"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO6654428.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18553..20061
                     /gene="ydgA"
                     /locus_tag="ACHKUB_001391"
     CDS             18553..20061
                     /gene="ydgA"
                     /locus_tag="ACHKUB_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6654429.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISFSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(20106..21371)
                     /gene="uidC"
                     /locus_tag="ACHKUB_001392"
     CDS             complement(20106..21371)
                     /gene="uidC"
                     /locus_tag="ACHKUB_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO6654430.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGGSVQKDVSNQIQSILEKGIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNDTA
                     LVSGQVTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(21410..22783)
                     /gene="uidB"
                     /locus_tag="ACHKUB_001393"
     CDS             complement(21410..22783)
                     /gene="uidB"
                     /locus_tag="ACHKUB_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO6654431.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWPLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22780..24591)
                     /gene="uidA"
                     /locus_tag="ACHKUB_001394"
     CDS             complement(22780..24591)
                     /gene="uidA"
                     /locus_tag="ACHKUB_001394"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO6654432.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFVLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(24979..25569)
                     /gene="uidR"
                     /locus_tag="ACHKUB_001395"
     CDS             complement(24979..25569)
                     /gene="uidR"
                     /locus_tag="ACHKUB_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO6654433.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25798..26565)
                     /gene="hdhA"
                     /locus_tag="ACHKUB_001396"
     CDS             complement(25798..26565)
                     /gene="hdhA"
                     /locus_tag="ACHKUB_001396"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO6654434.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(26677..27705)
                     /gene="malI"
                     /locus_tag="ACHKUB_001397"
     CDS             complement(26677..27705)
                     /gene="malI"
                     /locus_tag="ACHKUB_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO6654435.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            27880..29472
                     /gene="malX"
                     /locus_tag="ACHKUB_001398"
     CDS             27880..29472
                     /gene="malX"
                     /locus_tag="ACHKUB_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO6654436.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLIGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVRA"
     gene            29482..30654
                     /gene="malY"
                     /locus_tag="ACHKUB_001399"
     CDS             29482..30654
                     /gene="malY"
                     /locus_tag="ACHKUB_001399"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO6654437.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            30758..31759
                     /gene="add"
                     /locus_tag="ACHKUB_001400"
     CDS             30758..31759
                     /gene="add"
                     /locus_tag="ACHKUB_001400"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO6654438.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGHQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31794..32834)
                     /gene="ydgJ"
                     /locus_tag="ACHKUB_001401"
     CDS             complement(31794..32834)
                     /gene="ydgJ"
                     /locus_tag="ACHKUB_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6654439.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            33077..33202
                     /gene="blr"
                     /locus_tag="ACHKUB_001402"
     CDS             33077..33202
                     /gene="blr"
                     /locus_tag="ACHKUB_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO6654440.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            33475..33690
                     /gene="ydgT"
                     /locus_tag="ACHKUB_001403"
     CDS             33475..33690
                     /gene="ydgT"
                     /locus_tag="ACHKUB_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO6654441.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            33776..34216
                     /gene="ydgK"
                     /locus_tag="ACHKUB_001404"
     CDS             33776..34216
                     /gene="ydgK"
                     /locus_tag="ACHKUB_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO6654442.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            34293..34874
                     /gene="rsxA"
                     /locus_tag="ACHKUB_001405"
     CDS             34293..34874
                     /gene="rsxA"
                     /locus_tag="ACHKUB_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO6654443.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            34874..35452
                     /gene="rsxB"
                     /locus_tag="ACHKUB_001406"
     CDS             34874..35452
                     /gene="rsxB"
                     /locus_tag="ACHKUB_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO6654444.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            35445..37475
                     /gene="rsxC"
                     /locus_tag="ACHKUB_001407"
     CDS             35445..37475
                     /gene="rsxC"
                     /locus_tag="ACHKUB_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO6654445.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
     gene            37476..38534
                     /gene="rsxD"
                     /locus_tag="ACHKUB_001408"
     CDS             37476..38534
                     /gene="rsxD"
                     /locus_tag="ACHKUB_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO6654446.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            38538..39158
                     /gene="rsxG"
                     /locus_tag="ACHKUB_001409"
     CDS             38538..39158
                     /gene="rsxG"
                     /locus_tag="ACHKUB_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO6654447.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            39162..39857
                     /gene="rsxE"
                     /locus_tag="ACHKUB_001410"
     CDS             39162..39857
                     /gene="rsxE"
                     /locus_tag="ACHKUB_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO6654448.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            39857..40492
                     /gene="nth"
                     /locus_tag="ACHKUB_001411"
     CDS             39857..40492
                     /gene="nth"
                     /locus_tag="ACHKUB_001411"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO6654449.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT         9846 a        10486 c         9942 g        10279 t
ORIGIN      
        1 gccccgtatg tgttcccatc actgcggggc gttgtttttt gtgctagttg tgtgtaatgt
       61 gtatatacac ataaacacat tatatggggt gtaattgacg tgtcaacaca caaaaatgaa
      121 aggcgtggta atcctccttt ccagttccgt ttagatcctg acttacggga aaagatggaa
      181 aaagcacaac aacaagacgg tgatgactct ttagctgctt ggataaaacg aataatccga
      241 aaagaattac agcaacgcgg tattgaaccc aaagaataaa aaacatcagc gccgtggcgt
      301 gaggaactcc ggcgcattgc tttacaggta cacacaatga ccaataaaga atcaaccaat
      361 acaccatcgc aaaaaacgaa cagagagcac gaaaatatgg cgctcgagca tgaatcagaa
      421 agattcgctc catgcgcttt tgtccttgat gagtttctaa aaaaatattc acgttctgaa
      481 cggatgaaaa tggcagcaca atacggcccc aacaagcaag gtaattgccc accagcctga
      541 tagcggctat cattcccgtg cttatgtttg ggatcacata cacataaggc gcagcaggtt
      601 aattgttcag aaaggcggct ccatatcggg gccgcttttt ttatgcctga aaaaccccaa
      661 ttttgttgtt tttcagtttc accagggcga acgaatcccc gcccacgttc gggcgcatat
      721 tcaatcttca tagttatatc tctgtattag tggatgtgtg gcgactgtgt gccgccagtc
      781 tttttagtga actgccttgc agctatcctt ccaggccaaa acctcagata aagaccagcc
      841 aacagaacgc ccaccaagtt tacgacgtga tgggaattgt ccggcctttt ccaggcggta
      901 gcggcatgag cggctaaggc ctgttagctt ttcgcattct ttttcacgta taaaccgatc
      961 agtgcttaac actattgccc cctttcgttt cttaaagagt catcaggtgt cttattgtgt
     1021 cgtattgttc ccgctagtgt ggtgaatggc aaatgttgat gtcgtatggt ttacagaaag
     1081 aggaataatc aggataaaat catttaaatt catgttaata caaaggcata aaatcttgtt
     1141 ttatgccttt tttcgcacgc tttaacgcgt aattcactaa tgtataaaaa accagctgag
     1201 cattaaaaat cagcaactta caaatctgta ttctttttgg cctcttgttc gtgattgtgt
     1261 cacgttgttg cacattgttt cacgttgtat ctgtgcactt atccagtatg cgcatactga
     1321 aaaaacacga aaaaaattat tttattctgg ttactggtag cgtggttacg ttttcatgtg
     1381 ttcccgccag tatctccaac cgctccaccc acatatcaag cgcattgcgt ttcgcatcaa
     1441 tatagcggga atggttgtac acgcgttgca ttcctggcat ctggtggcct gtaagctgct
     1501 ccacgacgtg aggatcaacg cctaaatcgt tcagcatggt tgtaaaggtg cgccggatgt
     1561 catgcaatga ccattgaggg tgttttagcc tcctgtgcgc taatctgccg tactgcgata
     1621 cgctggcctc ctgtttcact tcccccagta ataagcccgt gtgcctgttc tggtccacca
     1681 gccgcgtgac gaacggcagg atcgcttccg gtatgggccg gaatattgca acctttgttt
     1741 tgctgtgctc cttcggaacg gtccatagca tttcggtaaa atcccactcg ctgatctccg
     1801 ataacctcag ttctaccgtc ctggctccga agacaatcag gaggcggatt aacgcgacgt
     1861 agtaaggaga aaatattttt ttatccagcg cctgcaataa ctcgcccagt tctttggtgc
     1921 ttaagacacg ttcgcttata tccggttttt tcccaacgtc cgccacgttc agatcatcga
     1981 gaacgttgct gattgcaaag cgccgcctcc ggcagaactt aagcgcctgc ttgcatgtct
     2041 gtagcacgaa tccggcagta acaggtgttc gctttgccac ctggtcaaaa caggccagcc
     2101 agtgccgtag ctcgcattta tccagcggca tagcgccaat gtactgtatt acgtgattat
     2161 taagtcgcct tttcagggcg atataatcca cgcggttttc ctttacgtac gactcaagcc
     2221 agtaggtgag cgcatcgcca accgttaccg gctttaacgc ttcctgtacg gcgtaattca
     2281 tctcatggcg tggatttttc ccctccgcca gccatgcgcg acactgggcg gctttttccc
     2341 tggctgcttt caggctcaga tcaggataat ttcccagctt aatacgttcc ggtcgtgtct
     2401 ctcttcccgt tcctgccctg taagtgaaat accaggttaa tttccctgat gttaaatatt
     2461 tcacgctcag atttccgcca tcgctataaa acgtgttttt ctccgctggc ttaccatgaa
     2521 gtttccttag caaggtatcg ctcagtttgt tcattgctct gctcacgttt taccccctgt
     2581 ttacgaaaaa gtgactacac cagtgactac accgatcgtt gcacaaaggg ctacaacgtg
     2641 aaacaagctg gaacaagaaa aatcacaaat ctgttgataa tcaaaaacat aatgaacaac
     2701 ctgaaacatt atgaaacggc aaaaaacact aattgataaa atgctgttat tcttaacccg
     2761 ctggtgcaac acatggtccg ctggcgagtg ccgcgtgtac tggcggtgtc gattttgatg
     2821 accatcatcg tgatggcgat ggtgttgcta ttagcttatc tgggttccgc gctcaacgag
     2881 ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca agctcttgag
     2941 ccgttgttgc aacgcgtagg gattgacgtc tcagttgacc agctggcgca ttacattgat
     3001 ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa tgccatgtca
     3061 tcaatatttt tattgctgct gacggtgctg tttatgctgc tcgaagtgcc acaattgccc
     3121 ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat tcaacgtgca
     3181 attgacagtg tttctcatta tctggtgctg aaaacagcca tcagcatcat caccggcctg
     3241 gtcgcctggg cgatgctcgc cgcactcgat gttcgcttcg cttttgtctg gggattgctg
     3301 gcctttgcgc ttaattacat ccctaatatt ggttcagttc tcgcggcaat cccccctatc
     3361 gctcaggtac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc gggatatctg
     3421 ctgattaatc tggtcttcgg caatattctg gagccgcgca tcatgggacg tgggctgggg
     3481 ctttccacat tggtggtatt tttgtcgttg attttttggg gatggttgtt aggaccggtg
     3541 ggtatgctgc tttccgtgcc gttaacaatt attgtcaaaa ttgcgcttga acaaacagcg
     3601 ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg gcctccgcag
     3661 aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg cgtcaccaaa
     3721 cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat agccagtgtt
     3781 catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca caggcatacc
     3841 agcaatagga ctcttcggat catcctgcgc cgccgggtta accgtatcgt tagcaccaat
     3901 caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt ccagcacgat
     3961 gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca aacgccccgc
     4021 aaccgggtgg ataccgaaac gcacgttgat accacgagcg cgcagtttct cggtaatttc
     4081 agcgacagga tattgcgcct gcgcgactgc catgccgtac cccggagtaa tgatcactga
     4141 atgggagttt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt gctcgcccac
     4201 ttcctgatca tcgccagtag aagagccgtc ggtgccgaaa ccacccgcaa taacgctgat
     4261 aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcgcccg aagaaccgac
     4321 cagcgcaccg gtcacaatca gcaggtcgtt gctgagcata aagcccgcag ccgcagccgc
     4381 ccagccggag tacgagttca gcatcgacac caccactggc atatctgcac caccgatgga
     4441 ggcgactaaa tgccagccga ataccagcgc aatggcggtc attatcagca atgccagcac
     4501 ttgcaggccg acgctatccg tgcgaacaaa tacaatcagc agcaggaagg aaacgaccag
     4561 agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg aaatcttgcc
     4621 acacagtttg ccgaacgcca ccaccgaacc cgtgaacgtt accgccccga tgaagatacc
     4681 gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg cgtcatgatg
     4741 cagataactg ttaaagccaa ccagcactgc cgccagaccc acgaagctat gcaggatcgc
     4801 caccagttct ggcatttcgg tcatttcaac tttcttcgcc agacggatac caattgcccc
     4861 accaatgacc atcgccagca agatccagcc aacattaccc gtatccggtc caaaaatggt
     4921 tgcgattaac gcaatcgcca tcccggcgat accgaagttg ttaccctggc gagacgtttc
     4981 atgtttcgaa agaccggcca gactgaagat aaacaggatc gcggcaacaa tgtatgcagc
     5041 tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt ttcagcatgc
     5101 gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc gcgataaaac
     5161 taaggaagct aacccagccg ccctggccaa tctgcaacag tgctccgaca acaataatcc
     5221 ctgaaatcgc gttggtgacc gacatcaacg gtgtatgcag cgcgtgcgat acattccaca
     5281 ccacgtaata accgacaacg caggccagcg cgaaaacggt gaagtgccca agaaactctt
     5341 tcggcgcaac gcttgacagc caaccgaaca ggatgattgc cagcgccatc aacgcgtatt
     5401 tacgccacgg tgagcaggta catttttcct cagttttcac ttccggtgcc gctttttgtg
     5461 ccgcctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt tcacccgcac
     5521 ggatcacggt cacgccgcga atcaccacat catcaaaatc aacagtgata ttgccgtctt
     5581 tctctttgca caacagtttc agcagattaa cgaggttagt accgtaaagc tgtgaggatt
     5641 gcgtcggcag acggcccgga agatcggtat aaccaatcac tttgacacca ttttccgtag
     5701 tgaagatttc acccggcacg gtgtattcac agttgccacc gttttgggct gccaggtcga
     5761 caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc ttcggcgctg
     5821 gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc tgggcggcaa
     5881 agagttccat ttccgcttta atgaacgcgt ccgacatcac tttggcatag ccatcgccgc
     5941 tgcccgcttc ctctttaaaa tccagctcga ggaattccgc gcccatactt tgaacttgtt
     6001 ctttcacttc cggacgggtg tcgaatgcac gcacaatcgc gccgagactg tttgctgcgc
     6061 caatggcggc cagacctgca acacccgcac caatcaccat cacttttgcc ggtggcactt
     6121 tcccggccgc agtaatttgc ccggtaaaga agcgcccaaa ttcatgtgcc gcttcaacaa
     6181 tggcgcgata accggcgatg ttcgccatcg agcttagtgc gtccagcgat tgtgcgcgtg
     6241 agatacgcgg cacagaatcc atcgccatca cggtcacgtt acgttccgca agtttttgca
     6301 ttaattccgg attctgcgca ggccagataa aactcaccag cgttgtccct ggattcagta
     6361 acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac tgccagacgc
     6421 tattcccttc tacaatttca gcgcccgctt gcacaaacgc tttatcgtca aaacttgcca
     6481 gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc tgttccactg
     6541 tttttggcgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt atgccaattc
     6601 gcatgatatt cccttccatc ggttttattg atgatggttt gcctgtgtca ggagccacac
     6661 aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa caaacgccct
     6721 ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtattt cggcgtgaac
     6781 tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa cgccggataa
     6841 agactgataa ttgtcttcga cggtcgggta aaacgagaca tcgccccggc acgaatcact
     6901 acttaacatt aaattaactt atacaattca gttgcttcag tagtaatgat gctgatacgg
     6961 ctgtttttta agcatagacg gtcatttgag caggattaaa attggcttaa ggaatgtgat
     7021 atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac gtttctcgtt
     7081 aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg aagcttaaga
     7141 acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac gcagcaacag
     7201 aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg gttaccggtc
     7261 gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat aaagaaggtg
     7321 ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac tggcgtgtgg
     7381 tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat cgcgtaatta
     7441 acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt gacacggtca
     7501 ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc aaagcagcaa
     7561 aagcgaaagg tgcctactct ttctacattg ttcgtcaaat tgatgccaac cagggcggca
     7621 accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc gtccagagcc
     7681 cggatgtgat cccggcagat tccgaagcag gacgtgcagc tctggctgcc ggtggcgaag
     7741 ccgcgaagaa agttgagatc ccaggtgttg cgactaccgc atcaccaagt tctgaagtcg
     7801 gtcgtttctt tgaaacccag tcatcaaaag gcggacgtta cactgtcacg ctcccggatg
     7861 gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg ttcgacagca
     7921 tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt gcgaaacgtg
     7981 ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcaggaa cgtggtaata
     8041 acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat aaggcggctt
     8101 gatgccgcct ttcgcatttt tagtcacaaa tttccccgcg accattgcat gcagacacat
     8161 ctcttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt tgccctaata
     8221 tttattcttc ttatcacgtt ttaatcactg gatatcgatg gaaaagaaac tgggactgag
     8281 cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca gtctgccgca
     8341 aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta ttactggcgc
     8401 tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgtc ccgaacttga
     8461 cggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct tttgttccgc
     8521 atggggatac tggctgtgcg cagtcatcgc caacgtctcc tatctggtga tcgttttttc
     8581 cgcgttaagc ttttttacgg acacgccgga attgcgcctg ttcggcgatg gtaatacctg
     8641 gcaatcgata gtcggtgcat cggctttatt gtggattgtt cactttttga tcctgcgcgg
     8701 ggtgcaaacc gctgccagca ttaacctggt ggcgacactg gcaaaattgt tgccgctggg
     8761 tctgtttgtt gtgctggcga tgatgatgtt caaactggat accttcaagc tcgacttcac
     8821 cggacttgca cttggcgtac ccgtgtggga acaggtgaaa aacaccatgc tgatcaccct
     8881 gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc gtaataaacg
     8941 tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt acttgctggt
     9001 aacgctgctt tcgctgggcg tggtggcacg tcctgaactg gctgaaattc gtaacccgtc
     9061 aatggccggt ctgatggtgg aaatgatggg accatggggc gaaatcatca tcgctgccgg
     9121 tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcgg cagaagttcc
     9181 gttcctggct gccactcata aagcattccc acgcattttt gcgcgccaga acgcacaagc
     9241 tgcgccatcc gcctcgctgt ggttgaccaa catctgcgtg caaatttgtc tggtactcat
     9301 ctggctgacc ggttccgatt acaacacgtt gctgaccatc gcctcagaaa tgattctggt
     9361 gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac tgcataaagc
     9421 ggtaggtgtc ggtgcctgca tttatggctt atggttattg tatgcctctg gtccaatgca
     9481 cttgctgtta tccgttgttc tgtatgcgcc ggggctgctg gttttcctgt atgcgcgcaa
     9541 aacgcataca catgataatg tactgaaccg tcaggaaatg gtgctgattg gcatgctgtt
     9601 aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatccc atcgttggat
     9661 agcaaaggag ataacgatgg gtaaagccca gcccttgcca atattaatta ctggcggagg
     9721 tcgtcgcatc ggcctcgccc tcgcctggca tttcattaat caaaagcaac cggtgattgt
     9781 cagctatcgg acgcactatc cagccattga tggactgatt aaggcaggtg cgcagtgtat
     9841 tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag tactaaaaag
     9901 cacccatggt ctgcgtgcta ttttgcataa cgccagtgcg tggatggcgg aaaaaccagg
     9961 tacaccactg gccgacgtac tggcttgcat gatgcagatc cacgttaata ccccatacct
    10021 gctcaaccat gcgctggaaa gattactgcg tgggcacgga cacgccgcca gcgatatcat
    10081 tcactttacc gattatgtgg tggagcgcgg tagcgacaaa catgttgcgt atgctgcaag
    10141 caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac cggaagtgaa
    10201 agtgaattct attgcgccat cgctgatcct gtttaatgaa catgatgatg ccgaatatcg
    10261 acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag aagtgatcga
    10321 cctggtcgat tacttactta ccagctgctt tgtcaccgga cgcagtttcc cacttgatgg
    10381 cggtcgtcat ctgcgttaat gcagctttat ccagcatata atcaacaccc atgcgcttat
    10441 cccccaacac gcaacagaac caacaaaagc ggcgggcaag cgcatcattc cggtgaaata
    10501 ccacagcgac accaggtaaa caaaataggg aataatcgac cacatactaa aaatgatggt
    10561 tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga taagcgcaaa
    10621 ggtcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg ctaaaacacc
    10681 aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca tctttttacc
    10741 ttaacacttc cataacaagt catcagtaga atacctgatg aaaacttgtt tagaaacgat
    10801 tgatagtaag taaaaacagc gcggtgtatt gtgacgtttt tatatctacc gtgaatgtta
    10861 tgaacactat cgtatttgtg gaagatgatg cggaagtcgg ttcactgatt gccgcgtacc
    10921 tggcaaaaca tgatatgcag gttaccgtag aaccgcgcgg cgaccaggcc gaagaaacca
    10981 ttttgcgaga aagtccggat ttggtgttac tcgacatcat gctaccaggc aaggacggca
    11041 tgaccatttg tcgtgattta cgcgcaaagt ggtctggacc gattgttctt ctaacctctc
    11101 tcgatagcga tatgaaccac atcctggcac tggaaatggg tgcctgcgac tatattctca
    11161 aaacgacgcc ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt cagaatgagc
    11221 aagccacact gaccaaagat cttcaggaaa cgtctctgac tccctacaaa gccctgcatt
    11281 tcggcacgtt gaccatcgat cccatcaacc gcgtagtcac cctggctaac actgaaatct
    11341 cgctctcgac agctgatttc gaattattgt gggaattagc tacccatgcc gggcaaatca
    11401 tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacgga ctggatcgta
    11461 gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc gcagaacctt
    11521 atcgcattaa aactgtgcgt aacaaaggct atctttttgc gcctcatgca tgggaataag
    11581 cgatgaaaaa actgtttatc cagttttacc tgttattgtt tgtctgcttc cttgtgatgt
    11641 ctctgctggt tgggttggtg tacaaattta ccgccgaacg cgcgggcaaa cagtcgctgg
    11701 atgatttgat gaacagttcg ctgtatctga tgcgcagcga attgcgtgag atccccccac
    11761 acgactgggg taagacgctg aaagagatgg atttaaatct ctctttcgat ctgcgtgtcg
    11821 agccactgag taaataccat cttgatgata tttccatgca ccgactgcgt ggcggcgaaa
    11881 ttgtcgccct ggacgatcag tacacgtttt tgcagcgtat cccgcgcagc cactacgtgc
    11941 tggcagttgg tcctgttcct tatctttatt atctccatca gatgcgattg ctggatatcg
    12001 ccctgatcgc ttttattgct atttccctcg cctttccggt gtttatctgg atgcgtccgc
    12061 actggcagga tatgttaaaa ctggaagcag cggcgcaacg atttggcgat gggcatctca
    12121 atgagcgtat ccactttgat gagggttcga gctttgaacg acttggtatc gcatttaacc
    12181 agatggcgga caatatcaac gccttgattg ccagcaaaaa acagcttatt gacggtatcg
    12241 ctcacgaact gcgaacaccg ttagtgcgcc tgcgttatcg actggagatg agcgataacc
    12301 tgagcgccgc cgaatcccag gcgttgaatc gtgatatcag tcaacttgaa gctttaattg
    12361 aagagctgct gacttatgcc cgactcgatc gcccacaaaa cgagcttcat cttagcgaac
    12421 cagacctgcc gttgtggctg tcaacgcatc tggcagatat tcaggcagta acgcccgata
    12481 aaacggtacg gataaaaacg ctcgtgcaag gccattatgc ggcgttggat atgcgcttaa
    12541 tggagcgcgt gctggataat ttgctcaata acgccctgcg gtactgccat tcaacggttg
    12601 aaaccagcct gctactgtcg gggaatagag cgacattaat tgttgaggat gatggcccag
    12661 ggattgcccc agaaaaccgc gaacatatct ttgaaccttt tgttcgcctc gatcccagcc
    12721 gggatcgctc aaccggcggc tgcgggctgg ggctggcaat tgtccactct atagcactgg
    12781 caatgggcgg tacggttaat tgtgacacca gcgaactggg tggtgctcgc ttctcgttta
    12841 gctggccgtt atggcataac atcccgcaat ttacctctgc ctgacactac gcgcacgatg
    12901 gtcaagtcac cacgactgtg ctataaaata agtatgttgt aactaaagtg gttaatatta
    12961 tggcgcgtta cgatctcgta gaccgactca acactacctt tcgccagatg gaacaagagc
    13021 tggctacatt tgccgctcat cttgagcaac acaagctatt ggttgcccgc gtgttctctt
    13081 tgccggaggt aaaaaaagag gatgagcata atccgcttaa tcgtattgag gtaaaacaac
    13141 atctcggcaa cgacgcgcag tcgctggcgt tgcgtcattt ccgccattta tttattcaac
    13201 aacagtccga aaatcgcagc agcaaggccg ctgtccgtct gcctggcgtg ttgtgttacc
    13261 aggtcgataa cctttcgcaa gcagcgttgg tcagtcatat tcagcacatc aataaactca
    13321 agaccacgtt cgagcatatc gtcacggttg aatcagaact ccccaccgcg gcacgttttg
    13381 aatgggtgca tcgtcatttg ccggggctga tcacccttaa tgcttaccgc acgctcaccg
    13441 ttctgcacga ccccgccact ttacgctttg gttgggctaa taaacatatc attaagaatt
    13501 tgcatcgtga tgaagtcctg gcacagctgg aaaaaagcct gaaatcacca cgcagtgtcg
    13561 caccgtggac gcgcgaggag tggcaaagaa aactggagcg agagtatcag gatatcgctg
    13621 ccctgccaca gaacgcgaag ttaaaaatca aacgtccggt gaaggtgcag ccgattgccc
    13681 gcgtctggta caaaggagat caaaaacaag tccaacacgc ctgccctaca ccactgattg
    13741 cactgattaa tcgggataat ggcgcgggcg tgccggacgt tagtgagttg ttaaattacg
    13801 atgccgacaa tgtgcagcac cgttataaac cacaggcgca gccgcttcgt ttgatcattc
    13861 cacggctgca cctgtatgtt gcagattaac gcccggcttt catactgccg accatctgtt
    13921 ctggccgtac ccagctgtca aactcggctt cgctaagata ccccagcgca agggccgcag
    13981 cttttaaggt cagaccttct ttatgcgctt ttttggcgat ctccgcggct ttgtcataac
    14041 caatgtgggt gttaagcgca gtcaccagca tcagcgattc attgagtaat tgattgattc
    14101 gctcacgatt cggttcaata cccactgcgc agtgtttgtt aaaactttcc atgccatctg
    14161 ccagcaagcg caccgattgc aggaaattat ggatcaccat cggacggaag acgttcagtt
    14221 caaagttacc ggaagcgcca cccatgttga tcgccacgtc gttccccatt acctgacagc
    14281 agagcatggt taatgcttca cactgggttg ggttcacttt ccccggcatg attgagctgc
    14341 ccggctcatt ttccgggatt gagatttcac caattccgca gcgcgggcca gaggccagcc
    14401 agcggacatc attggcgatt ttcatcagtg acgcagccaa ccctttcaac gcgccgtgcg
    14461 cctgaaccag ggcatcacag gtcgccagcg cttcaaattt gttcggcgcg gtaacaaacg
    14521 gtgcacaggt aatgactgcc agttcatctg ctacgcgacg cgcatactcc ggatgggtat
    14581 ttagtccagt acccaccgct gtaccgccaa gagccagttc cgctacgtga ggcaggctgt
    14641 attcgatatg tttgagatta tgctcgagca tcgctaccca gccggaaatc tcctgcccca
    14701 gtgttagcgg cgtggcgtcc tgcaagtggg ttcgaccgat ttttacgata tcggcaaatg
    14761 cacgcgattt ttcactcagc gtctgtgtca gggttttaag ctgcggaatg agttgcttgc
    14821 gcagcgccag cagcgccgca acgtgcatcg ccgtcggaaa gacatcgttg gaactttggc
    14881 ttttgttcac gtcgtcgtta gggtgaactt tacgttccat cccgcgcacg ccgccgagta
    14941 attcactggc ccggttagcc agcacttcgt tcatgttcat gttactttgc gtgccggagc
    15001 cggtctgcca gatagccagc gggaattcgt cgtcatgctg tcctgccagt acttcatccg
    15061 ccgcctgccg aatggcgctc gctttctctt cagacaacaa gcctaaatct tcattaactt
    15121 ttgccgctgc gcgcttggtt agcgccagcg catgaatcag tgaggtgggc attttctccg
    15181 tcgaaatgcg gaaatgctcc agcgagcgtt gagtttgtgc gccccacagc ttatctgccg
    15241 ggacatcaat cgcccccatc gaatcttttt cgctgcgtac tgtattcatg gcctgctcct
    15301 cacctgttta attttttctt tctgttttgc tttcgttaag caacttttag ctcacttatt
    15361 atttactatt tgataacaaa tgtttagtct ttcgtgccat gtaaaaaaac cgccccgaag
    15421 ggcggctctg tttatttcac acagcgagtg cactgtgtga gttgtatctg ctggaagaag
    15481 tcatttcctt tatcatccac aaggataaac gccgggaaat cttccacttc aattttccag
    15541 atggcttcca ttcccagttc cggatattca acacattcca ggctcttaat acttccctgc
    15601 gccaatacag cggccggacc accgatactg ccaaggtaga agccgccgtg ttttttacag
    15661 gcatccgtca cctgctggct gcggttgcct ttcgccagca tgatcatact tccgccctgc
    15721 gcttgcagtt gatcgacata agaatccatc cgtccggcgg tcgttgggcc aagagaaccg
    15781 gaggcataac cttccggcgt tttggccgga cccgcgtagt aaatcggatg atctttaatg
    15841 tactgcggca gcccttcacc gttatccatc cgctctttca gtttggcgtg agcaatatca
    15901 cgaccgacga taatcgtgcc attcagcgat aagcgtgtag aaacgggata ctgcgacaac
    15961 tgtgcgagga tctctttcat cggacggtta aggtcaacgc gcaccgcttc gccttctccc
    16021 gctttgcgca gctcttccgg gatatatttg cctgggttat gttccagttt ttcgatccag
    16081 atcccctgac ggttgatctt cgctttgata ttacggtcag cagagcagga gacgcccata
    16141 ccgaccgggc aggatgcgcc gtgacgtggc aggcgaatca cgcggatgtc gtgagcgaag
    16201 tatttaccac caaactgcgc acccagacca agattttgcg cttcgatcag caattctttt
    16261 tccagttcca catcgcggaa cgcctgaccg tgctcattcc cttccgttgg cagttcatca
    16321 tagtatttag cggaagccag tttgaccgtt ttaaggttcg tttctgcaga agttccacca
    16381 ataacgaacg caatatgata cggaggacag gccgccgtac ccagcgtgcg catcttctca
    16441 accaggtaat ttttcagttt ccccggcgtt agtaacgctt tggtttcctg atagagatac
    16501 gtcttgttag ccgaaccgcc acctttggcg atacagagga atttgtactc gtcgccatca
    16561 acggcataaa gatcgatctg cgctggtaga ttggtgccgg tattcacctc tttatacata
    16621 tccagcggcg cgttttgcga gtagcgcaga ttatcttcga tataagtgtt atagacaccg
    16681 cgtgccagcg ccgcttcatc accgccaccg gtccatacac gctgcccttt tttaccaaca
    16741 ataatcgcgg tgccggtatc ctgacaggtt ggcagaacgc ctttcgccgc gatgtcggag
    16801 ttgcgcagga attgcagcgc cacatattta tcattttcgc tggcttccgg gtcacgcaga
    16861 atgtcggcca cctgttgttg gtgagccgga cgcagcataa atgacgcatc atgaaatgcc
    16921 tgacgcgcca acagagttaa cgcttcgggt gcgactttca aaatctcctg cccttcaaat
    16981 tcagatacgc taacgtgttc gctggttagc aggtaatact cagtatcatc ttttttgagt
    17041 ggaaaaggag cctgataatg aaagggtttg tttgacattg ttctctcact tactgcctgg
    17101 tttggttatg ctctgggcgg gtgttccgtt gccctgttaa aagcgagtaa caatatccta
    17161 cacacttttt taacaaaaac tgagactagt acgacttttt gcggctccag gttacttccc
    17221 gtaggattct tgctttaata gcgggattaa tttccacatt aaaacaggga ttgatcatgc
    17281 aaaaactcat taactcagtg caaaactatg cctggggcag caaaacggcg ttgactgaac
    17341 tttatggtat ggaaaatccg tccagccagc cgatggccga gctgtggatg ggagcacatc
    17401 cgaaaagcag ttcacgggtg cagaatgccg ccggagatat cgtttcactg cgtgatgtga
    17461 ttgagagtaa taaatcgact ctgctcggag aggccgttgc caaacgcttt ggcgaactgc
    17521 ctttcctgtt caaagtatta tgcgcagcac agccgctctc cattcaggtt catccaaaca
    17581 aacgcaattc tgaaatcggt tttgccaaag aaaatgccgc aggtatcccg atggatgccg
    17641 ccgagcgtaa ctataaagat cctaaccaca agccggagct ggtttttgcg ctgacgcctt
    17701 tccttgcgat gaacgcgttt cgtgagtttt ccgagattgt ctccctactc cagccagtcg
    17761 caggtgcaca tccggcaatt gctcactttt tacaacagcc taatgccgaa cgtttaagcg
    17821 aactgttcgc cagcctgttg aatatgcagg gtgaagaaaa atcccgcgcg ctggcgattt
    17881 taaaatcggc gctcgacagt cagcagggtg aaccgtggca aacgattcgt ttaatttctg
    17941 aattttaccc ggaagacagc ggcctgttct ccccgctgtt actgaatgtg gtgaaattga
    18001 atcctggcga agcgatgttc ctgttcgctg aaacaccgca cgcttacctg caaggcgtgg
    18061 cgctggaagt gatggcgaac tccgataacg tgctgcgtgc gggtctgacg cctaagtaca
    18121 ttgatattcc ggaactggtt gccaatgtga aattcgaagc caaaccggct aaccagttgt
    18181 tgacccagcc ggtgaaacaa ggtgcagaac tggacttccc gattccagtg gatgattttg
    18241 ccttctcgct gcatgacctt agtgataaag aaaccaccat tagccagcag agtgccgcca
    18301 ttttgttctg cgtcgaaggc gatgcaacgt tgtggaaagg ttctcagcag ttacagctta
    18361 aaccgggtga atcagcgttt attgccgcca acgaatcacc ggtgactgtc aaaggccacg
    18421 gccgtttagc gcgtgtttac aacaagctgt aagagcttac tgaaaaaatt aacatctctt
    18481 gctaagctta ttaaaggctt ataacacctt caggcggcca gtccgcctga tttcatttta
    18541 tggataatca ttatgaataa atcgctggta gcggtaggcg tcattgttgc gctaggcgta
    18601 gtctggacag gcggcgcatg gtatacaggc aagaagattg aaacccatct cgaagacatg
    18661 gtcgcgcagg cgaacgcgca actcaaactg acagctcctg aatccaacct ggaagtgagt
    18721 tatcaaaact atcatcgcgg cgtattcagc agccagttgc aactgttggt gaaacccatt
    18781 gccgggaaag aaaatccgtg gattaaaagc ggtcagagcg tcatcttcaa cgaatcggtt
    18841 gatcatggtc ccttcccgct tgcccagctt aaaaaactga acctgatccc gtcgatggca
    18901 tcaattcaaa ccacgctggt taataacgaa gtaagcaaac cactgtttga tatggcaaaa
    18961 ggtgaaacgc cttttgagat taactcgcgc attggttaca gcggtgattc cagttccgat
    19021 atttcgctca agccactgaa ttacgagcaa aaggatgaaa aagtcgcctt tagcggcggc
    19081 gagttccagt taaatgcgga cagagacggc aaagctatct ccttttccgg ggaggcgcaa
    19141 agtggtcgga tagacgcagt taacgaatac aaccagaaag tgcagttgac ctttaataat
    19201 ctgaaaaccg acggttccag cacgctggca agttttggtg agcgcgtagg aaaccaaaaa
    19261 ctgtcactgg aaaaaatgac catttcggtg gaaggcaaag aactggcact gctggaaggc
    19321 atggagatca gcggtaaatc ggatctggtc aatgacggta aaacgatcaa tagccaactg
    19381 gattactcgc taaacagcct gaaggtacag aatcaggatc tgggcagcgg caagctgact
    19441 ttaaaagtcg gccagattga tggtgaagcc tggcatcagt ttagccagca atataacgcg
    19501 caaactcagg cgctgctggc acagccagaa attgccaaca atcccgaact ttatcaggag
    19561 aaagtgacgg aagccttctt tagcgccctg ccgctgatgt tgaaaggcga tccggtgatt
    19621 actatcgcgc cgctaagctg gaaaaacagt cagggtgaaa gtgcgctgaa tctgtcgctg
    19681 ttcctgaaag atccggcaac gactaaagaa gcgccgcaaa cgctggcgca ggaagtagat
    19741 cgttcggtta aatctctgga tgcgaaactg accattccgg tggatatggc aactgagttt
    19801 atgactcagg tagcgaagct ggaaggttat caggaagatc aagcgaaaaa actggcgaaa
    19861 cagcaagttg aaggtgcatc agcaatgggg cagatgttcc gtctgaccac cttgcaggac
    19921 aataccatca ccaccagcct gcaatatact aacggtcaga taacgttaaa cgggcagaaa
    19981 atgccactgg aagatttcgt tggtatgttt gcaatgccgg cattaaatgt tccggtcgta
    20041 cccgctattc cgcagcagta attcacagac aaaacgccgg agatttttct ctccggcgtt
    20101 atcttttact tcagcataaa gtcatactga atactcattt tcacatccca gtaattcagg
    20161 cgatcgccca gcgtactgcc atcgcgcttt tcccgccctt tatcggcagc aagaaagaaa
    20221 ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccacctaa gatacccgcg
    20281 ccttcaatcc gcacatctcg tttactggat tcatagccgc gagtcacaat cggtgcaaat
    20341 gtcagtgtaa attgcggcgt taaacgatag ttgacgccca attgccagga ctgcattcct
    20401 tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtatc caccacggca
    20461 ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca cggcgcattc
    20521 caggtcactt gtccactaac caacgccgta tcattgggct ggctggtatt acgacttaaa
    20581 ccttcgagtt gcgcgtaaaa gccgagcaac tcccctttca acgtataatt cgggtcgagc
    20641 ggtatgcctt tctcgagaat actttgtatc tgattggaaa catctttctg taccgatccc
    20701 cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga acgtgcgcga
    20761 tgtttatcgt attttcccgt ctggctgtta tacacccctt catcacgaat accggttgtt
    20821 gaatcggtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat atttggcgtt
    20881 aacgcccaat aacctaatgc tccttcagaa actgtcggta atgtacgttg cgaactgcca
    20941 gccattaacg gaatacgata aggtaagcta ccgtaactat aatccgtccc gaagcgacct
    21001 aatttaagca ggctattttc actgggtttt atttttactg cccccagggc aaagccaaaa
    21061 cttttatcac catcaagata ccaccgggta ctcatatcag cacgagcacc taatttataa
    21121 acataatacg ccccgccttc aacgccaata atattggaat aataaccact attgaaatcg
    21181 agcaatcctc cctgtaccca tgcgtaaata tctcttcccg cgccagcact gggcttatca
    21241 gccctgcgca attcgttttt cagtctgatg cgtagtcccg cttcatcgtc agccagttgc
    21301 gccgcataag cagaggtaag gccagaggca gccgtcaggc aaataacggc catggccact
    21361 atttttctca tagttgcacc tttgatgttc tgattatttt tattgaatat taattagtga
    21421 tatcgctgat taattgctgc tgcacttttt tacgattatc aatttcaacc acgatttctt
    21481 tgaatttttt atccgtgagc ggataaaacc agataataac gaatgccagt agcataaatc
    21541 cgcaaggtac taaggcaatt gatgtgcgga tgcccataat aacttccggc gtttgcacct
    21601 gattggcgat atatccgctt aatcccaaaa taaaggcagg aattgaacct ccgattgcct
    21661 gaccgcattt acgggtaaat gagaatagtg aataggtgag cccttcaatt cgcacgccgg
    21721 tcaggtattc accgtattct acggtatcag cttccagtgc ccacatcaca gtcatagtaa
    21781 cgccctgacc aattgaagcg atggccaacg caaccaacgc caccggcagt ggccagacgg
    21841 aaacccagaa gaatagcaga taaccgcagg ttcccagcaa agcgccaatc aggaaggtat
    21901 tctttttacc gatcctcgcg accatccccg gcaccagcgg tgccgatgcc acagtaccaa
    21961 ccaggttttg caccagtacc agcacagtga acagcccggt atcatttaac acatagcgca
    22021 cgtagaacaa cgacgaggcg ctgaccgcga aggtcgaaat cagcacacac agcgcaccga
    22081 tgcacaacat aaacagcggg cgattccgtt tcagggtttg cagactgatc ttcaatgacg
    22141 gctgcgccac gatacgtacc acattctcac gcgtcgattt gaagcagatg aagtaaagca
    22201 ccattccggc aatcgccagc acaatcgtcc agaaatggta taccgacacc atctcttccg
    22261 ggctggagtt cttaatgctc ggtcctatca gaaatgccag gcagacaaag gttaatgaag
    22321 cggcaatccc gcgagccgcg cccagacggg cgcgggattg tggttgttgg gtcatcgcgg
    22381 tagcaagtga accataagga atattcacca ggctgtagca aagcccgagg cccatgtagg
    22441 tcaaatatgc atacactact ttgctgctat ggctccagtc ggtcggtacc cagaatacca
    22501 gcacgctgaa gatcattaac ggcgcagtac cgaagagtaa aaacgggcgg aattttcccc
    22561 agcgggtatt cacactgtcc accactcgtc cggcaaagac gtcggcgaag gcatcgaata
    22621 cccgcaccag taacagcatg gtgcccgccg cagcggcacc gacgccagcg acgtcggtgt
    22681 agtaactcaa taggaagagc gcccccattg cgaaggcgaa gttattggcg acgtcaccga
    22741 ggctgtagcc gacgatggtg cgccaggaga gttgttgatt cattgtttgc ctccctgctg
    22801 cggtttttca ccgaagttca tgccagtcca gcgtttttgc agcacaaaag ccgccgactt
    22861 cggtttgcgg tcgcgagtga agatcccttt cttgttaccg ccaacgcgca atatgccttg
    22921 cgaggtcgca aaatcggcga aattccatac ctgttcaccg acgacggcgc tgacgcgatc
    22981 aaagacgcgg tgatacatat ccagccatgc acactgatac tcttcactcc acatgtcggt
    23041 gtacattgag tgcagcccgg ctaacgtatc cacgccgtat tcggtgatga taatcggctg
    23101 atgcagtttc tcctgccagg ccagaagttc tttttccagt accttctctg ccgtttccaa
    23161 atcgccgctt tggacatacc aaccgtaata acggttaagg cacagcacat caaagagatc
    23221 gctgatggta tcggtgtgag cgtcgcagaa cattacattg acgcaggtga tcggacgcgt
    23281 cgggtcgagt ttacgcgttg cttccgccag tggcgcgaaa tattctcgtg caccttgcgg
    23341 acgggtatcc ggttcgttgg caatactcca catcaccacg cttgggtggt ttttgtcacg
    23401 cgctatcagc tctttaatcg cctgtaagtg cgcctgctga gtttccccgt tgactgcctc
    23461 ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta aagagaggtt
    23521 aaagccgaca gcagcagttt catcaatcac cacgatgcca tgttcatctg cccagtcgag
    23581 catctcttca gcgtaagggt aatgcgaggt acggtaggag ttggccccaa tccagtccat
    23641 taatgcgtgg tcgtgcacca tcagcacgtt atcgaatcct ttgccacgca agtcagcatc
    23701 ttcatgacga ccaaagccag taaagtagaa cggtttgtgg ttaatcagga actgttcgcc
    23761 cttcactgcc actgaccgga tgccgacgcg aagcgggtag atatcacact ctgtctggct
    23821 tttggctgtg acgcacagtt catagagata accttcaccc ggttgccaga ggtgtggatt
    23881 caccacttgc aaagtcccgc tggtaccttg tccagttgca accacctgct gatccgcatc
    23941 acgcagttca acgctgacat caccatttgc taccacctgc cagtcaacag acgcgtggtt
    24001 acagtcttgc gcaacatgcg tcaccacggt gatatcgtcc acccaggtgt tcggcgtggt
    24061 gtagaccatt acgctgcgat ggatcccggc atagttaaag aaatcatgga agtaagactg
    24121 ctttttcttg ccgttttcat cggtaatcac cattcccggc gggatagtct gccagttcag
    24181 ttcgttgttc acacaaacgg tgatacgtac acttttcccg gcaataacat acggcgtgac
    24241 atcggcttca aatggcgtat agccgccctg atgctccatc acttcctgat tattgaccca
    24301 cactttgccg taatgagtga ccgcatcgaa acgcagcacg atacgctggc ctgcccaacc
    24361 tttcggtata aagacttcgc gctgatacca gacgttgccc acataattac gaatatctgc
    24421 atcggcgaac tgatcgttaa aactgcctgg cacagcaatt gcccggcttt cttgtaacgc
    24481 gctttcccac caacgctgat caattccaca gttttcgcga tccagactga atgcccacag
    24541 gccgtcgagt tttttgattt cacgggttgg ggtttctaca ggacgtaaca taagggactc
    24601 ctcattcaaa taaaaatact ggtcaacctt taatctgatt agttaaccaa ttctgtgttg
    24661 cacacagtta tctgtgagaa acccatggaa gagaaatgga aataaaaaga actgacaccg
    24721 cgatcaaaaa accaaaatct gcaataccca cagcgtgata tagatcgcat taatctttaa
    24781 aacagcgttg acagaagcca taaaataagc aatcgaacgt tcgttcgagc atcttgagaa
    24841 cgataaactc tcatgacata tggctacggt gaatattttt ggggggaatt ctccgatccc
    24901 ggctagattg tattttgtac agggaaatta aagtatgaaa cgcgggcaca gttatcacca
    24961 gcccgcgaaa tgagaggatc aggatgcggt taagataccg ccaatcatcg cgcgtaatcc
    25021 ctgagcaaac gacgcttcac gcgccaggtc ttccgcttcg atatcggcca gtacaccgta
    25081 ggtcagatcc agcagtaaac gtgaagtcat tgacgtgttg atgtctgggc ttatctcgcc
    25141 tttttgctgg gcatcacgca tccgctgggc aacaaattca gtgatcgtca tatgcttatt
    25201 tttaagcatg gcggcgacct gtgggttacg catcccttcc gccataattt cgaccaccag
    25261 cgcccgttgc ccaaaggctt catgggtgag agagacaata gactcgacca tatagtcaac
    25321 aaaatgaatc ccttcaatcg gttcccggaa acgggccagc gccctctcct ggtcctgtaa
    25381 gataatcgcc tgaatcaagg cttctttgga gatgaaatgg tgatagagcg tcccgggact
    25441 aatggcgcag gatttacaga tggctttcat cgaggcactg tgaaatccat tttctgaaaa
    25501 aatctctctg gcagcattga ggatccgggt ccgtgtcggt tgtgcttcag tctgcatgtt
    25561 atccatcatt aaaccattat agttagcgtg aataactagt ttaccgcgtt gtcaggtgag
    25621 cgcctatcat tgctgttgaa tagtgcgaag gcacactcta ttcgtatgct tttcatccag
    25681 ctattggtta accaatagcc gtgatttggc tgccagttgt ttagcagaga cgttgatgaa
    25741 ttgcacacgc tatcccggta aacaacacat acggctgttt accggttcgt tagtgtatta
    25801 attgagctcc tgtaccccac caccggagac ggtgagaatt tgtccgctta cccagctggc
    25861 tgcaggcgag caaaggaaca gcgccgcgtt agcaatatct tgcggttggc ccagacgtct
    25921 gatcggcgtg tgctgtaaca ttttttgttc aatttctggt gtaataacgg atttcagggc
    25981 atcggttaat attgcccccg gcgcaatgcc attcacccga atattttttt cacccaggtc
    26041 aaatgccata tttctgacca gatgactggc cgcagcttta gatgatgcat aggaagtcat
    26101 gtttatattt ttattttctg ccgccataga agtgatggtc agaataacgc caccgccatt
    26161 tttttccatt tctggcgcaa caagttgtga cagatggaaa aaagaaaaca cattcagttc
    26221 ataagcacgg cgaaaatctg ccattggcat atcaaacggt ttaggaccac cgccaccggc
    26281 gttgttaacc aggatatcaa ctttacccag cttactgacg gcaaagtctg ccagtgcaga
    26341 gagttcctgt tcggaagtaa tatcacaacg gcaggcaaat gcctgaccac ccagttgttg
    26401 aatttcgtct acaacatggt tagctgcgtc ggcgttaata tcactgacca ccacagatgc
    26461 gccagctgtc gcgaatgtaa tggcgatttc tttaccaata cctgcacccg cacctgtgat
    26521 gatggcgcat tttccgtcga gtctcaggtt gtcagaatta aacacataaa cctcctgtga
    26581 acttcagtaa aaataaaagg caccgcctgt gtgccacatt ttttatcatt aaccttgagg
    26641 aaaggacact accaggtata gcagataaag agaggattat ttcgccgcaa tgagccgggc
    26701 gggaataata agattgcgtg aatgcgtctc ttcatgggtg attttttgca tcatgcgatc
    26761 cgcaagtgtg gtaccaagtt cccgcgcagg cgtgctggcc caggtaacgg gtatatcatc
    26821 aagtgtggtt ggtgtcgcat cggtaaatgc cgccagcgaa acttgttgct caaagtaacg
    26881 atcgactccg ctttccccgc tttgccgccc tgctttgagc aaaccaaacc atgcccccat
    26941 cgcaatagtt tcgttatagc aaaccacagc actgatggtc gggttatgac gtaaaagcgc
    27001 cgtgatagct tccgcggctt gcttctggct ggatgtgcac tccagcaccc aatcgctgtg
    27061 aaacggcagg ccaaatttta gtagagttgc acaatagccc cccacccgtt ctgcacgggt
    27121 taatgaggaa ctttgccctc ccagccaggc gatccgctga tgcccattgc gaatgagatg
    27181 ctccgtcaac aactgtgcag cctgcatgtt gtccgggcga accgtatcaa catcatcaag
    27241 ataactggca cgggaagcga aaatcaccgg gatagctttt tcttctgcca tccgtcgcag
    27301 gtcatcgctg cttcctgcag ccccggcaat taccacacca tccacaccct gattcagtaa
    27361 cagtgaaaac cgctgtgcca gctgctcgcc gtctttaccg ccgtgaagca aaaaaaccat
    27421 ccgtccctgc gcttccagag cttccgtcaa tccggccgtc aattcggcgt aaaacggcgc
    27481 agataaatca cggacgatca aaccaatgac gccgctttgc ccgccgcgca gcgccgacgc
    27541 ctggcgattg cgcacaaatc ccagctcttc aatggcggcg ttaacgcgtt ctcctgtggc
    27601 ggtagagatt cgccctttgc cactaagcac cagcgaaacg gtacttaccg acacgcccgc
    27661 agccagcgca acatcatgaa tggttatttt tttggcggta gccatgcaga tgacctactc
    27721 cctgattatg tgacagataa aacgttttac cttttattta tcttataccc gctattatcg
    27781 ttgcgtaatg tgatttatgc ctcactaaaa tttgataaaa cgttttatct tctcgcgcaa
    27841 tttactgaat ccagattgtt ctctacgagg agtcgtttta tgacggcgaa aacagcaccg
    27901 aaagtcacgc tgtgggagtt cttccagcag ttaggcaaaa ccttcatgtt acccgtggca
    27961 ttattgtcgt tctgcggcat tatgctcggc attggtagtt ctcttagcag ccatgatgtc
    28021 ataaccctga tcccggtcct gggcaacccc gtgttgcagg ctatctttac ctggatgagt
    28081 aagattggct cgtttgcttt tagtttcctg cctgtcatgt tctgtatcgc catcccgctg
    28141 ggcctggcac gcgaaaataa aggcgtagcg gcattcgctg gcttcgtcgg ttatgcggta
    28201 atgaacctcg cggtaaactt ctggttgacc aataaaggca ttctgccaac cacggatgcc
    28261 gcggttctga aagccaataa catccagagc attcttggga tccagtcgat cgataccggg
    28321 atcctcggtg cggtgatcgc cggtattatc gtctggatgc tgcatgagcg tttccataat
    28381 atccgcctgc cggatgcgct ggcattcttc ggcggtacgc gcttcgtacc aattatctcc
    28441 tcgctggtga tgggccttat cggcctggtg attccattag tctggccgat tttcgccatg
    28501 ggtattagcg gcttgggcca tatgataaac agcgcgggtg atttcggacc gatgctgttt
    28561 ggtaccggtg aacgtctgct gttgccgttt ggtctgcatc acattctggt ggcattaatt
    28621 cgctttaccg acgcaggcgg cacgcaggaa gtctgcggtc aaaccgtcag cggcgcactg
    28681 accatcttcc aggcgcaatt gagttgcccg accactcacg gtttttctga aagcgccacg
    28741 cgtttccttt cgcaaggtaa aatgcctgcg tttctcggcg gtctgccagg tgcagcgtta
    28801 gctatgtatc actgcgcgcg cccggaaaat cgccataaaa ttaaaggtct gctgatttct
    28861 ggcctgatcg cctgtgtcgt tggcggcacg accgaaccgc tggaattcct gttcctgttc
    28921 gtggcgccag ttctgtatgt catccacgcg ctgttaaccg gcctcggctt cactgtcatg
    28981 tctgtgctcg gcgtcaccat cggtaatacc gacggcaata tcatcgactt cgtggtgttc
    29041 ggtattttgc atggtctgtc aaccaagtgg tacatggtgc cagtggtggc ggcaatctgg
    29101 tttgtcgttt actacgtcat cttccgtttc gctatcaccc gcttcaatct gaaaaccccg
    29161 gggcgcgata gcgaagttgc cagctcaatc gaaaaagccg ttgccggtgc gccgggtaaa
    29221 tcaggttaca acgttcctgc aatcctcgaa gcattaggcg gtgccgacaa tattgtcagc
    29281 ctcgataact gcattacccg tctgcgtttg tctgtgaaag atatgtcgct tgttaatgtg
    29341 caggcactga aggacaatcg ggcaattggc gtagtacaac ttaatcaaca taacctgcag
    29401 gttgttatcg ggccacaagt tcagtcagta aaagatgaaa tggccggtct gatgcatact
    29461 gtccgggcat aaggataaga tatgttcgat ttttcaaagg tcgtggatcg tcatggcaca
    29521 tggtgtacac agtgggatta tgtcgctgac cgtttcggca ctgctgacct gttaccgttc
    29581 acgatttcag acatggattt tgccactgcc ccctgcatta tcgaggcgct gaatcagcgc
    29641 ctgatgcacg gcgtatttgg ctacagccgc tggaaaaacg atgagtttct cgcggctatt
    29701 gcccactggt tttccaccca gcattacacc gcaattgata cgcagacggt ggtgtatggc
    29761 ccttctgtca tctatatggt ttcagaactg attcgtcagt ggtctgaaac aggtgaaggc
    29821 gtggtgatcc acacgcccgc ctatgacgca ttttacaagg ccattgaagg taaccagcgc
    29881 acagtaatgc ccgttgcttt agagaagcag gctgacgact ggttttgcga tatgggcaag
    29941 ctggaagccg tgttggcgaa accagaatgt aaaattatgc tcctgtgtag cccacagaat
    30001 cctaccggga aagtgtggac gtgcgatgag ctggagatca tggctgacct gtgcgagcgt
    30061 catggtgtgc gggttatttc cgatgaaatc catatggata tggtctgggg cgagcagccg
    30121 catattccct ggagtaatgt ggcgcgggga gactgggcgt tgctgacgtc gggctcgaaa
    30181 agtttcaata ttcccgcgct gaccggtgct tacgggatta tagaaaatag cagcagccgt
    30241 gatgcctatt tatcggcact gaaaggacgt gatgggcttt cttccccttc ggtactggcg
    30301 ttaactgccc atatcgccgc ctatcagcaa ggtgcaccgt ggctggatgc cttacgcgtc
    30361 tatctgaaag ataacctgac gtatatctca gataaaatga acgccgcgtt tcctgaactc
    30421 aactggcaga tcccgcaatc cacttatctg gcctggcttg atctacgtcc gttgaatatt
    30481 gacgacaacg cgttgcaaaa agcgcttatc gagcaagaaa aagtcgcgat catgccgggg
    30541 tatacctatg gtgaagaagg tcgtggtttt gtccgtctca atgccggctg cccacgttcg
    30601 aaactggaaa aaggtgtggc tggattaata aacgccatcc gcgctgttcg ctaaccccaa
    30661 ttgcgcaacg taaaaaatcg ttgcgcaatc gtggattttt accctgcttt gtttttataa
    30721 tggtgcgcac ttttatattc agaaaaagag tgcgaccatg attgatacca ccctgccatt
    30781 aactgatatc catcgccacc ttgatggcaa cattcgtccc cagaccattc ttgaacttgg
    30841 ccaccagtat aatatctcgc ttcctgcaca atccctggaa acactgattc cccacgttca
    30901 ggtcattgcc aacgaacccg atctggtgag ctttctgacc aaacttgact ggggcgttaa
    30961 agttctcgcc tctcttgatg cctgtcgccg cgtggcattt gaaaacattg aagatgcagc
    31021 ccgtcacggc ctgcactatg tcgagctgcg tttttcacca ggctacatgg caatggcaca
    31081 tcagctgcct gtagcgggtg ttgtcgaagc ggtgatcgat ggcgtacgtg aaggttgccg
    31141 cacctttggt gtgcaggcga agcttatcgg cattatgagc cggaccttcg gcgaagccgc
    31201 ctgtcagcaa gagctggagg cctttttagc ccaccgtgac cagattaccg cacttgattt
    31261 agccggtgat gaacttggtt tcccgggaag tctgttcctt tctcacttca accgcgcgcg
    31321 tgatgcgggc tggcatatta ccgtccatgc aggcgaagct gccgggccgg aaagcatctg
    31381 gcaggcgatt cgtgaactgg gtgcggagcg tattggacat ggcgtaaaag ccattgaaga
    31441 tcgggcgctg atggattttc tcgccgagca acaaattggt attgaatcct gtctgacctc
    31501 caatattcag accagcaccg tagcagagct ggctgcacat ccgctgaaaa cgttccttga
    31561 gcatggcatt cgtgccagca ttaacactga cgatcccggc gtacagggag tggatatcat
    31621 tcacgaatat accgttgccg cgccagctgc tgggttatcc cgcgagcaaa tccgccaggc
    31681 acagattaat ggtctggaaa tggctttcct cagcgctgag gaaaaacgcg cactgcgaga
    31741 aaaagtcgcc gcgaagtaac aaaatggatg gtgcaaattg caccatccat ttttcatgca
    31801 aggcacaaag tcgcgcgatg tttggcggat tcgatgccca gctcaatcaa ctccattacc
    31861 tggattgcct ggcttgccgg aaccggattt tcaccatcgc catttaacgc atcacgaata
    31921 gccgcatagt aagccggata attcccaggc accgtcaaca gcgtttcttc gacacgttcc
    31981 tcaccttcca cgcgggtaag tacgccatca cgcatatcgt agccccagtc ttcctgcggt
    32041 agacgctcgc catttttcag acgttcttcc tgtggatcga ggccatattt cacataactg
    32101 cctcgggatc catgcacgat ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt
    32161 aaaatgactc gccgctgtgg ataggacaag atggcgtgga aataatcggt cgactgcgct
    32221 ccgggccgta actgtgccaa atctaccgtc atgctgaccg gtaaaccaaa aagcgtaatg
    32281 gcctgatcaa gaagatgtgg tgctaaatcg taccagatac cgctgcctgg gccgccctgt
    32341 tcacgccaac gatcgcgcac ctgcggacgg aagcggtcaa aatgagactc aaagtaagca
    32401 acttcaccca gcacgccttc cgcgagtaaa ccttttagcg tcaagaaatc gctatcccag
    32461 cgacggttat ggaatacaga cagcacacgc cccaggcttt ttgccagtgc atccagctct
    32521 cgcgcttgtg acagtgtcac ggtaaagggt ttatcaacga ccacatgttt acccgcctca
    32581 agcgccgctt tggctaacgg gaaatgggta tcgttgggtg taggaatgac aatcaggtct
    32641 atgttgggat cgttaaacag atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg
    32701 gcttttactt ttgtttcatc actgctggag attaccgcca gttccagccc gggcgtgccc
    32761 gcaatcaggg gcgcatggaa ggttttgctc gcataaccat acccaatcaa cccaacacgg
    32821 atgttgtcgc tcatgtcatt gcctctcatt gaagtatgat ggctatttga cactatcctt
    32881 tacccacgct caacagttta ataacctgcc agcaataagg gatgttgttt aacttaagtc
    32941 aaaaaaatag cgaattttcc aacgacaaaa gctaaatatc gcaaaaacct cagtaaaaat
    33001 cttgctggag ctattattgc taagtaacat ttaccccctg aagttaatgg atcaatcaag
    33061 agagatgtgg gctgtaatga atcgtcttat tgaattaaca ggttggatcg ttcttgtcgt
    33121 ttcagtcatt cttcttggcg tggcgagtca cattgacaac tatcagccac ctgaacagag
    33181 tgcttcggta caacacaagt aagctctgca cttgtggagc gacatgctgc ccgtccgggt
    33241 gcatgttttc acttgtcgga tattaaacca ggaatttatt atcttgttcg atgttgttgg
    33301 tgattgtcag ggatagtaaa gcgcgttact cttttcagag cagtttttca tccctgaata
    33361 tttgccatca gcgcattctt attattgtaa taacacaagc gttcactttt ggtgaaattt
    33421 tatcatcact aataatacct acccctttgg tattaattat tttatggacc ttttatgact
    33481 gttcaggact acttattaaa atttcgcaaa atcagttcac tcgaaagtct ggaaaaactc
    33541 tacgaccatc ttaattacac cctgacggac gatcaggaac tgatcaatat gtatcgtgct
    33601 gccgatcacc gtcgcgcaga gctggtttct ggcgggcgtt tgtttgacct cggccaggta
    33661 ccgaagtccg tctggcacta tgtccaataa agaaagtagc gatcggttat aaaagcctta
    33721 taataacgcc cctaaaatta gatttgcctg tttgcgtcgc tattctggag aacctatgac
    33781 caccacaacg ccacaacgta ttggaggctg gttgcttggc ccgttggcct ggcttttagt
    33841 cgctctgttg agtacgacgc tggcgttgtt gctgtatacc gctgcgttat cttctccgca
    33901 aacatttcaa acgctcggcg gacaagcact gacgacacaa atcttatggg gcgtttcttt
    33961 tattaccgct atcgccatgt ggtattacac actgtggttg accatcgctt tctttaaacg
    34021 tcggcgttgt gttcctaaac actatattat ctggctgctt atttccgtat tgctggcagt
    34081 aaaagcattc gctttttcac ccgtggaaga tggcatagcc gttcgccagt tgctgtttac
    34141 tttgttggca accgcattga tcgtccccta tttcaaacgc tcgtcgcggg taaaagcgac
    34201 gtttgtgaat ccgtaataac cttacagtta acctgttgtc gcctgctctg gattaacgga
    34261 taataggcgg cttttttatt tcaggccgaa aaatgactga ctacctgtta ctgtttgtcg
    34321 gaactgtact ggtcaataac tttgtactgg tcaagtttct cggtctctgt ccgtttatgg
    34381 gggtttccaa aaaactggaa accgcgatgg gcatggggct ggcaacaacg tttgtgatga
    34441 cgctggcgtc tatttgcgcc tggcttatcg atacgtggat tttgatccca cttaatctga
    34501 tttacctgcg caccctggca tttattctgg tgattgctgt ggtcgtgcag ttcaccgaga
    34561 tggtggtgcg caaaaccagc ccggtgcttt accgtctgct ggggattttt ttgccgctta
    34621 tcaccaccaa ctgtgcagtg ctcggcgtgg cgttgctgaa tatcaatctc gggcacaatt
    34681 tcttgcagtc ggcgctgtac ggtttttccg ccgctatcgg tttctcgctg gtgatggtgc
    34741 tcttcgccgc catccgcgaa cgccttgctg tggctgatgt cccggcacct tttcgcggta
    34801 atgccattgc gttaattacc gcaggtctta tgtctctggc ctttatgggc tttagtggtt
    34861 tggtgaagtt gtaatgaatg ctatctggat tgccgttgcc gccgtgagcc tgctgggcct
    34921 ggcgtttggc gccattctgg gttatgcctc ccgccgtttt gcggtggaag acgatccggt
    34981 cgttgagaaa attgacgaaa tcttaccgca gagccagtgt ggtcagtgcg gttatcccgg
    35041 ctgtcgcccc tacgcggaag ccatcagctg taacggtgaa aaaatcaacc gttgcgcccc
    35101 aggtggcgaa gctgtgatgc taaaaattgc cgagttgctt aatgtcgagc cgcagccgct
    35161 ggatggcgaa gcgcaagagc taacgcctgc gcggatggtg gcggttattg atgaaaataa
    35221 ctgtattggc tgcactaaat gtattcaggc ttgtccggta gacgccatcg ttggcgctac
    35281 ccgtgccatg catacggtaa tgagtgatct ctgtacgggc tgcaatttat gtgttgatcc
    35341 gtgcccgacg cactgcatct cgttgcaacc ggtcgcagaa acacctgact cctggaaatg
    35401 ggatctgaac accattcccg tgcgtatcat tcccgtggaa caccatgctt aagttattct
    35461 ctgcattcag aaaaaataaa atctgggatt tcaacggcgg catccatcca ccggagatga
    35521 aaacccagtc caacggtaca cccctgcgcc aggtaccact ggcgcagcgt tttgttattc
    35581 cactgaaaca gcatattggc gctgaaggtg agttgtgcgt tagcgtcggc gataaagtat
    35641 tgcgcggcca gccgcttacc cgtggtcgcg gcaaaatgct gcctgttcac gcgcccacct
    35701 cgggtaccgt tacggctatt gcgccccact ctacggctca tccttcagct ttagctgaat
    35761 taagcgtgat tattgatgcc gatggtgaag actgctggat cccacgagac ggctgggccg
    35821 attatcgctc tcgccgtcgc gaagagttaa tcgagcgcat tcatcagttt ggtgttgccg
    35881 ggctgggcgg tgcgggattc ccgacaggcg ttaaattgca gggcggcgga gataagattg
    35941 agacgttgat tatcaacgct gctgagtgcg agccgtacat taccgccgat gaccgtttaa
    36001 tgcaggattg cgcggctcag gtcgtagagg gtattcgcat tcttgcgcat attctgcagc
    36061 cacgcgaaat tcttatcggc attgaagata acaaaccgca ggcgatttcc atgctgcgcg
    36121 cggtgctggc ggactctcac gatatttctc tgcgggtgat tccaaccaaa tatccttctg
    36181 gcggtgctaa acaattaacc tacattctga ccgggaagca ggttccacat ggcgggcgtt
    36241 catccgatat cggcgtatta atgcaaaacg tcggcactgc ttatgcagtg aaacgtgccg
    36301 ttattgatgg cgagccgatt accgagcgtg ttgtaaccct gactggcgaa gcaatcgctc
    36361 gcccgggcaa cgtctgggca cggctgggga cgccagtgcg tcatttattg aatgatgccg
    36421 gattctgccc ctctgccgat caaatggtga ttatgggtgg cccgctaatg ggctttacct
    36481 tgccatggct ggatgtcccg gtcgtaaaga ttaccaactg tctgttggct ccctctgcca
    36541 atgaacttgg cgaaccacag gaagaacaaa gctgcatccg gtgtagcgcc tgtgctgacg
    36601 cctgccctgc ggatcttttg ccgcaacagt tgtactggtt cagcaaaggt cagcaacacg
    36661 ataaagctac cacgcataac attgctgatt gcattgaatg tggggcttgc gcgtgggttt
    36721 gcccgagcaa tattcccctg gtgcaatatt tccgtcagga aaaagctgaa attgcggcta
    36781 ttcgtcagga agaaaagcgc gccgcagaag ccaaagcgcg tttcgaagcg cgccaggctc
    36841 gtctggagcg cgaaaaagcg gctcgccttg aacgacataa gagcgcagcc gttcaacctg
    36901 cagccaaaga taaagatgcg attgctgccg ctctggcgcg ggtgaaagag aaacaggccc
    36961 aggctacaca gcctattgtg attaaagcgg gcgaacgccc ggataacagt gcaattattg
    37021 cagcacggga agcccgtaaa gcgcaagcca gagcgaaaca ggcagaactg cagcaaacta
    37081 acgacgcagc aaccgttgct gatccacgta aaactgccgt tgaagcagct atcgcccgcg
    37141 ccaaagcgcg caagctggaa cagcaacagg ctaatgcgga accagaagaa caggtcgatc
    37201 cgcgcaaagc cgccgtcgaa gccgctattg cccgtgccaa agcgcgcaag ctggaacagc
    37261 aacaggctaa tgcggaacca gaagaacagg ttgatccgcg caaagccgcc gtcgaagccg
    37321 ctattgcccg tgccaaagcg cgcaagctgg aacagcaaca gactaacgca gaaccagaag
    37381 aacaggttga tccgcgcaaa gcggcagttg ccgcggctat tgcccgcgct caggccaaaa
    37441 aagccgccca gcagaaggtt gtaaacgagg actaaatggt attcagaata gctagctccc
    37501 cttataccca taaccagcgc cagacatcgc gcattatgct gttggtgttg ctcgcagccg
    37561 tgccaggaat cgcagcgcaa ctgtggtttt ttggttgggg tactctcgtt cagatcctgt
    37621 tggcatcggt tagtgctctg ttagccgaag ctctcgtact caaactacgc aagcagtcgg
    37681 tagccgcaac gttgaaagat aactcagcat tgctgacagg cttattgctg gcggtaagta
    37741 ttccccccct cgcgccatgg tggatggtcg tgctgggtac ggtgtttgcg gtgatcatcg
    37801 ctaaacagtt gtatggcggt ctggggcaaa acccgtttaa tccagcaatg attggttatg
    37861 tggtcttact gatctccttc ccggtgcaga tgaccagctg gttaccgcca catgaaattg
    37921 cggtcaacat ccctggtttt atcgacgcca tacaggttat tttcagcggg cataccgcca
    37981 gtggtggtga tatgaacaca ctacgcttag gtattgatgg cattagtcag gcgacaccgc
    38041 tggatacatt taaaacctct gtccgtgccg gtcattcggt tgaacagatt atgcaatatc
    38101 cgatctacag cggtattctg gcgggcgctg gttggcaatg ggtaaatctc gcctggctgg
    38161 ctggcggcct gtggttgcta tggcagaaag cgattcgctg gcatattccc ctcagcttct
    38221 tagtaacgct ggcgttatgc gcaacgttgg gctggttgtt ctcaccagaa acactggcag
    38281 caccgcaaat tcatctgctg tctggtgcga ccatgctcgg cgcattcttt attttgactg
    38341 acccggttac cgcttctacg accaatcgtg gtcgtcttat tttcggcgcg cttgcgggct
    38401 tattagtctg gttgatccgc agtttcggcg gctatcctga cggcgtggct tttgccgtcc
    38461 tgctggcgaa catcacggtt cctctgatcg attactacac gcgtccgcgc gtctacggcc
    38521 atcgcaaagg gtaaaccatg ctgaaaacta tccgaaaaca cggcattacg ttagcgctat
    38581 ttgcagcggg ttcaacaggg ttaactgcgg ccatcaacca gatgaccaaa acgacgattg
    38641 ctgaacaggc cagtctgcaa caaaaggcat tatttgatca ggtgctgcca gccgaacgct
    38701 ataacaatgc gctggcacag agttgctatc tggtaactgc gccagagtta ggtaaaggtg
    38761 agcatcgggt ttacatcgcc aaacaggatg acaaaccggt agccgccgtt ctggaagcaa
    38821 ccgcgccaga tggctattcc ggtgcgattc agctgctggt gggagccgat tttaacggca
    38881 cggtacttgg cacgcgcgtg acagagcacc acgaaacgcc agggcttggc gataaaatcg
    38941 aactgcgcct ttctgactgg atcacccatt ttgcgggtaa aaaaatcagt ggtgcagatg
    39001 atgcgcactg ggcggtgaag aaagatggtg gtgatttcga ccagttcacc ggcgcgacga
    39061 ttactccccg cgcggtggtt aatgcggtaa aacgcgccgg attgtacgct cagacgttac
    39121 cggcacaact ttctcaactt cctgcctgtg gagaataaag cgtgagcgaa attaaagacg
    39181 ttattgttca ggggttgtgg aaaaacaact ctgcgctggt ccagttgctc ggcctttgtc
    39241 ctctgttggc ggtcacgtcc actgccacta acgctctggg tttaggactt gcgactacgc
    39301 tggtactgac gctgaccaac ctgaccattt cgacgctgcg tcactggacg ccagccgaga
    39361 tccgcattcc catttacgtg atgatcatcg cctcggtggt cagcgctgta cagatgctga
    39421 tcaacgccta cgcctttggc ctgtatcaat cattagggat ttttattccg ctgattgtca
    39481 ctaactgtat cgttgtgggc cgcgctgaag ccttcgccgc caaaaaaggt ccggcgcttt
    39541 cggcactgga cggcttttca attggtatgg gcgcaacctg cgccatgttc gtgctgggtt
    39601 cactacgcga aattatcggc aatggcacat tgtttgacgg tgcagatgcg ctgttaggta
    39661 gctgggcaaa agtattacgc gtggaaattt tccacaccga ctcccctttc ctgctggcga
    39721 tgctgcctcc aggtgcattt attggcctgg gactgatgct ggcaggaaaa tacctgattg
    39781 atgaaagaat gaaaaagcgc cgtactgaag cagctgccga acgtgcattg ccaaacggtg
    39841 aaacagggaa tgtctgatga acaaagcaaa acgcctggag atcctcactc gcctgcgtga
    39901 gaacaatcct catcccacca ccgagcttaa tttcagttcg ccttttgaat tgctgattgc
    39961 cgtactgctt tccgctcagg cgaccgatgt cagtgttaat aaggcgacgg cgaaactcta
    40021 cccggtggcg aatacgcctg cagcgatgct tgaactgggc gttgaagggg ttaaaaccta
    40081 tatcaaaacg attgggcttt ataacagcaa agcagaaaat atcatcaaaa cctgccgtat
    40141 cttactggag cagcataatg gcgaggttcc ggaagatcgt gctgcgcttg aagccctgcc
    40201 cggcgtaggt cgtaaaacag ccaacgtcgt attaaacact gcattcggct ggccgactat
    40261 tgctgtcgac acgcacattt tccgcgtttg taatcgtact caatttgcgc cggggaaaaa
    40321 tgtcgaacag gtagaagaaa agctactgaa agtggttccg gcagagttta aagtcgactg
    40381 ccaccattgg ttgatcctgc acgggcgtta tacctgcatt gcccgcaagc cccgctgtgg
    40441 ctcttgtatt attgaagatc tttgtgaata caaagagaaa gttgacatct gaagaaaagg
    40501 ggtaacaccg attaccccat tgataacctt tctttatcct cttttaaaac atc
//
LOCUS       DAZFRN010000028        39834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFRN010000028 DAZFRN010000000
VERSION     DAZFRN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.028"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            28..399
                     /locus_tag="ACHKUB_001412"
     CDS             28..399
                     /locus_tag="ACHKUB_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654450.1"
                     /translation="METKEIRRLRLKEWFKDRTLPPKEKSYLSQLMSGKATFGEKAAR
                     RIEQTYGMPGGYLDIEPEDITESPSSRSIALTPNQLELLQIFSAFPDNEQQEIIKELK
                     NKKEAMEDLVARWLARQGRRA"
     gene            419..880
                     /locus_tag="ACHKUB_001413"
     CDS             419..880
                     /locus_tag="ACHKUB_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6654451.1"
                     /translation="MGVLEISALRIKMLLLAKGWSQAGLARRIGIAQQSVQRWVCGIS
                     SPTAANLDKLSEVTGLPQYWFFLPLNEEKKDRAQDILKITPNQKELLQTFEAFPEEDQ
                     QQMLKDMKEKKEAMDRIVARWLAAQQKVNQAEYQHLQNEVHHEHSPFTDGF"
     gene            849..1040
                     /locus_tag="ACHKUB_001414"
     CDS             849..1040
                     /locus_tag="ACHKUB_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO6654452.1"
                     /translation="MNTALSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VERRMAERFAKMRKERSEQ"
     gene            1041..1328
                     /locus_tag="ACHKUB_001415"
     CDS             1041..1328
                     /locus_tag="ACHKUB_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6654453.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWKRLRTCVQPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTISGIEIVNVVHARRQFPVTP"
     gene            1606..1758
                     /locus_tag="ACHKUB_001416"
     CDS             1606..1758
                     /locus_tag="ACHKUB_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6654454.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            complement(1751..1942)
                     /locus_tag="ACHKUB_001417"
     CDS             complement(1751..1942)
                     /locus_tag="ACHKUB_001417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654455.1"
                     /translation="MEETPAILTAAPFRGLPQPEVHGRRKFNDAVQIYELRRALSCYA
                     LTFQGKLTSGNLFLLVLIS"
     gene            2248..2436
                     /gene="dicB"
                     /locus_tag="ACHKUB_001418"
     CDS             2248..2436
                     /gene="dicB"
                     /locus_tag="ACHKUB_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6654456.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            2433..2621
                     /locus_tag="ACHKUB_001419"
     CDS             2433..2621
                     /locus_tag="ACHKUB_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6654457.1"
                     /translation="MSSAFALMMTVFLITGEPQNVITGIYASKGSCLQARDEQKISGE
                     CLPLNKVSLYLNNETPAG"
     gene            2717..5152
                     /locus_tag="ACHKUB_001420"
     CDS             2717..5152
                     /locus_tag="ACHKUB_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6654458.1"
                     /translation="MSKIFICAAIPDEQAIKNEGAVAVATAIKAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGTHRPALDSWDAEYMLENRWDEESASFVPVETESDPM
                     NVTFDKLSPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPEIKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     KANQQYTENSTGKISPVIAAIHREYRQTWKTLDDELAYALWPGDVDAGNIDGNIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALLYSRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEHEETDQSPADALHLSAEQTDSVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNTDISEHSEQ
                     PEPVKVTADEVNKIMQAANISQPDSDKLLAASRGEFVEGISDPNDPKWVKGIETCDSV
                     NQNQSETEQNDQKAEQNSPNALQDEPETKQSEPVAQQEEEKVCTACGQSSGGNCPDCG
                     AVMGNATYQETLDEENQAAAQEEMEGSGGDHYHTTDNESGETANPLIKVNGHRESTST
                     SRLWHHLMIDLETMGKNPDAPINALAGKFFDPATGEMGPEFSKTIDLETAGGVIDRDT
                     IKWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVILRR
                     SYERQEIPCPWRYTNDRDVRTIVALGLVMDFDARSVITFEGERHNALHDARYQAKYVS
                     AIWQKLFPNQADF"
     gene            5219..5470
                     /locus_tag="ACHKUB_001421"
     CDS             5219..5470
                     /locus_tag="ACHKUB_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO6654459.1"
                     /translation="MYELTLSPAEIQEITRYERYTKQQQQLRLHGIPFVPGPKNEPIV
                     LRSDVPRGLTAIPKVSELVSADPDFEALNNGKTKKKQKR"
     gene            5439..6458
                     /locus_tag="ACHKUB_001422"
     CDS             5439..6458
                     /locus_tag="ACHKUB_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6654460.1"
                     /translation="MGRPRKNKKDNALPPRVRSNGYSYVWKPEGSTKTIGLGRVRETT
                     IAKVWQNYELEKAKRHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQRALLAVFGK
                     VLADNVKIEQVRIFMDKRGIESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFT
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSGTCEYVINTTKGGKVIAKTLNNWWNQAKHAA
                     EKKSGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            6867..7526
                     /gene="yccA"
                     /locus_tag="ACHKUB_001423"
     CDS             6867..7526
                     /gene="yccA"
                     /locus_tag="ACHKUB_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO6654461.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            7617..7946
                     /gene="tusE"
                     /locus_tag="ACHKUB_001424"
     CDS             7617..7946
                     /gene="tusE"
                     /locus_tag="ACHKUB_001424"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO6654462.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(7943..8221)
                     /gene="yccX"
                     /locus_tag="ACHKUB_001425"
     CDS             complement(7943..8221)
                     /gene="yccX"
                     /locus_tag="ACHKUB_001425"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO6654463.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            8316..9506
                     /gene="rlmI"
                     /locus_tag="ACHKUB_001426"
     CDS             8316..9506
                     /gene="rlmI"
                     /locus_tag="ACHKUB_001426"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO6654464.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9564..9881
                     /gene="hspQ"
                     /locus_tag="ACHKUB_001427"
     CDS             9564..9881
                     /gene="hspQ"
                     /locus_tag="ACHKUB_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO6654465.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(9926..10339)
                     /gene="yccU"
                     /locus_tag="ACHKUB_001428"
     CDS             complement(9926..10339)
                     /gene="yccU"
                     /locus_tag="ACHKUB_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO6654466.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            10512..11174
                     /gene="yccT"
                     /locus_tag="ACHKUB_001429"
     CDS             10512..11174
                     /gene="yccT"
                     /locus_tag="ACHKUB_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO6654467.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            11270..11728
                     /gene="mgsA"
                     /locus_tag="ACHKUB_001430"
     CDS             11270..11728
                     /gene="mgsA"
                     /locus_tag="ACHKUB_001430"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO6654468.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(11760..13814)
                     /gene="helD"
                     /locus_tag="ACHKUB_001431"
     CDS             complement(11760..13814)
                     /gene="helD"
                     /locus_tag="ACHKUB_001431"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO6654469.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQEKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            13937..14383
                     /gene="yccF"
                     /locus_tag="ACHKUB_001432"
     CDS             13937..14383
                     /gene="yccF"
                     /locus_tag="ACHKUB_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO6654470.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            14393..16555
                     /gene="yccS"
                     /locus_tag="ACHKUB_001433"
     CDS             14393..16555
                     /gene="yccS"
                     /locus_tag="ACHKUB_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO6654471.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPIYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(16518..17147)
                     /gene="sxy"
                     /locus_tag="ACHKUB_001434"
     CDS             complement(16518..17147)
                     /gene="sxy"
                     /locus_tag="ACHKUB_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO6654472.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            17366..17875
                     /gene="sulA"
                     /locus_tag="ACHKUB_001435"
     CDS             17366..17875
                     /gene="sulA"
                     /locus_tag="ACHKUB_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO6654473.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            18232..19284
                     /gene="ompA"
                     /locus_tag="ACHKUB_001436"
     CDS             18232..19284
                     /gene="ompA"
                     /locus_tag="ACHKUB_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO6654474.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(19359..19811)
                     /gene="matP"
                     /locus_tag="ACHKUB_001437"
     CDS             complement(19359..19811)
                     /gene="matP"
                     /locus_tag="ACHKUB_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO6654475.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            19997..21757
                     /gene="ycbZ"
                     /locus_tag="ACHKUB_001438"
     CDS             19997..21757
                     /gene="ycbZ"
                     /locus_tag="ACHKUB_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6654476.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            21826..22344
                     /gene="fabA"
                     /locus_tag="ACHKUB_001439"
     CDS             21826..22344
                     /gene="fabA"
                     /locus_tag="ACHKUB_001439"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO6654477.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(22414..22581)
                     /gene="rmf"
                     /locus_tag="ACHKUB_001440"
     CDS             complement(22414..22581)
                     /gene="rmf"
                     /locus_tag="ACHKUB_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO6654478.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(22837..23400)
                     /gene="pqiC"
                     /locus_tag="ACHKUB_001441"
     CDS             complement(22837..23400)
                     /gene="pqiC"
                     /locus_tag="ACHKUB_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO6654479.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            complement(23397..25037)
                     /gene="pqiB"
                     /locus_tag="ACHKUB_001442"
     CDS             complement(23397..25037)
                     /gene="pqiB"
                     /locus_tag="ACHKUB_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO6654480.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(25042..26295)
                     /gene="pqiA"
                     /locus_tag="ACHKUB_001443"
     CDS             complement(25042..26295)
                     /gene="pqiA"
                     /locus_tag="ACHKUB_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO6654481.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(26425..28332)
                     /gene="uup"
                     /locus_tag="ACHKUB_001444"
     CDS             complement(26425..28332)
                     /gene="uup"
                     /locus_tag="ACHKUB_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6654482.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(28344..30452)
                     /gene="rlmKL"
                     /locus_tag="ACHKUB_001445"
     CDS             complement(28344..30452)
                     /gene="rlmKL"
                     /locus_tag="ACHKUB_001445"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO6654483.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            30696..31805
                     /gene="ycbX"
                     /locus_tag="ACHKUB_001446"
     CDS             30696..31805
                     /gene="ycbX"
                     /locus_tag="ACHKUB_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO6654484.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(31802..32344)
                     /gene="zapC"
                     /locus_tag="ACHKUB_001447"
     CDS             complement(31802..32344)
                     /gene="zapC"
                     /locus_tag="ACHKUB_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO6654485.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(32518..33528)
                     /gene="pyrD"
                     /locus_tag="ACHKUB_001448"
     CDS             complement(32518..33528)
                     /gene="pyrD"
                     /locus_tag="ACHKUB_001448"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO6654486.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(33639..34376)
                     /gene="ycbF"
                     /locus_tag="ACHKUB_001449"
     CDS             complement(33639..34376)
                     /gene="ycbF"
                     /locus_tag="ACHKUB_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO6654487.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(34342..34857)
                     /gene="ycbV"
                     /locus_tag="ACHKUB_001450"
     CDS             complement(34342..34857)
                     /gene="ycbV"
                     /locus_tag="ACHKUB_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6654488.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(34865..35407)
                     /gene="ycbU"
                     /locus_tag="ACHKUB_001451"
     CDS             complement(34865..35407)
                     /gene="ycbU"
                     /locus_tag="ACHKUB_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6654489.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(35419..36489)
                     /gene="elfG"
                     /locus_tag="ACHKUB_001452"
     CDS             complement(35419..36489)
                     /gene="elfG"
                     /locus_tag="ACHKUB_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6654490.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(36480..39080)
                     /gene="elfC"
                     /locus_tag="ACHKUB_001453"
     CDS             complement(36480..39080)
                     /gene="elfC"
                     /locus_tag="ACHKUB_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO6654491.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(39105..39806)
                     /gene="elfD"
                     /locus_tag="ACHKUB_001454"
     CDS             complement(39105..39806)
                     /gene="elfD"
                     /locus_tag="ACHKUB_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6654492.1"
                     /translation="MKTCITKGIVTVSFTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT        10078 a        10177 c         9515 g        10064 t
ORIGIN      
        1 tttgcgatta ctaaagataa tgtatgtatg gaaacaaaag agattcggcg tttacgcctc
       61 aaagagtggt tcaaagacag gactttgccg cctaaagaga aaagttactt atctcagtta
      121 atgagcggta aagctacgtt tggcgaaaag gcagctagac gtattgaaca aacatatggg
      181 atgcctgggg gatatcttga tattgaacca gaagacataa cagagtctcc atcatcaaga
      241 tctatagcat taacccctaa ccaattagaa ttgctacaaa tatttagtgc tttccctgat
      301 aatgagcagc aggagatcat aaaagaacta aaaaacaaaa aagaggctat ggaagatctt
      361 gtcgcacgtt ggttggcacg acaaggtcgc cgagcctgac gggatatata gatatcagat
      421 gggcgtactc gaaattagtg ctcttagaat taagatgctt ctattggcaa aaggctggag
      481 ccaagcagga cttgcgcgca gaattggtat tgctcaacaa tccgtgcaac ggtgggtttg
      541 tggtatctca agccctacgg cagctaatct agataaacta tctgaagtaa cgggacttcc
      601 tcagtattgg tttttcttac cattaaatga agaaaaaaaa gacagggcac aggatatatt
      661 aaaaataacc cctaatcaaa aggaactact tcaaacattt gaggcatttc cagaggaaga
      721 tcaacagcaa atgcttaaag atatgaagga gaaaaaagaa gcaatggatc gtatagttgc
      781 aaggtggtta gcagcacaac aaaaagtcaa ccaagcagaa taccagcact tacaaaacga
      841 ggttcaccat gaacacagcc ctttcaccga tggtttctga atttgaaact gtcgagcaag
      901 aaaacagtta caacgaatgg ctgcgtgcga aagtagcagc gagccttgca gatccgcgcc
      961 cagcaattcc ccatgacgag gttgagcgta gaatggcaga acgctttgct aaaatgcgca
     1021 aggaacggag tgagcagtaa atgttacccg tgttatggct tgaaagcgca gataccgact
     1081 tggatgatat aaccagctat attgcacgct ttgacataga tgcggcagaa cgtttatgga
     1141 aacgcttaag gacttgcgtt cagccattat ctgagcatcc gtatttatac ccgccaagtg
     1201 acagagtgcc aggtttgcgt gaaattgtag ctcaccccaa ttacattatt ctgtaccgag
     1261 tgacaatttc aggcattgaa attgttaacg tagttcacgc ccgccgacag ttccctgtaa
     1321 caccttaatc cccctaaagc aaaaccttcc ccctctcaag agggagggtt attcccgccc
     1381 atcagttaag ttagatattg accgtttagt ttatttttgt ataaactaat ttcaccaacc
     1441 caccctgccc cacagaacgc caggcaatac ttcgagttac ccggcagtgg tcaggggtta
     1501 agtagccagc ccgaggcgta tgaacatgac ggcgggaaca ctttatataa cagcgcagca
     1561 ggtttttagt tccgcgcccc ggcgttaagg ggaaatgagg tcaacatgga tacgctcaat
     1621 cttggcaaca acgaatctct ggtatgcggt gtgttcccta accaggacgg cacgtttacc
     1681 gcgatgacgt ataccaaaag taaaaagttt aaaaccgaag ctggcgcgcg tcgctggtta
     1741 gccagaaaca ctaactgatt agcaccagta aaaacaggtt gccactggtt aatttaccct
     1801 gaaaagtcag ggcataacac gaaagcgcac ggcgaagttc gtatatctgt acggcgtcgt
     1861 taaattttct tcgaccgtgc acttccggtt gtggcaatcc gcgaaatggc gcggcggtaa
     1921 gtatggctgg ggtttcctcc attgctccag aaaatgcacc gggttgtcag gttgaccata
     1981 cgcttaagtg acagccccgc cacaataccc atgtgtagtc tttggtggca tcagttctac
     2041 tccgtgactg ctctgccacc ctttttaaag tgaattttgt gatgcggtga atgcggctaa
     2101 gcgcacgcgg aacagttaaa accgtaaagt ggtcttttac tggcgtaaca gacatcaact
     2161 aacaatccgg cgttaattgt taactggtta acgtcacctg gaggcaccag gcaccacatc
     2221 acaaaattca ttgttgagga cgcgataatg gaaacgtcac taccaaacgt taatacgtct
     2281 gaagggtgtt ttaatattgg tattctgctc agtaaccggg agtttactga ggacgccatc
     2341 aggatgagaa aatatgagcc ttatcttctc aatgataatt ccatactctc cagaattgcc
     2401 cttcttgaac ttggcatttt cggagggcag cagtgagttc agcgtttgca ctgatgatga
     2461 cggtttttct tataacaggt gagccacaga atgtgattac cggaatttat gccagtaaag
     2521 gatcctgcct ccaggcaaga gacgagcaaa aaatttctgg tgaatgtctc ccgttaaaca
     2581 aagtatcgct gtacctgaat aacgaaacac cggctggata acccaccagc catattaacg
     2641 ccataccaac ggattaaaaa tgccagcaat ggcagggatt cgttcaccct gaaatctgta
     2701 atgaggttta aacacaatga gtaaaatatt tatttgcgcc gccattcctg acgaacaggc
     2761 aataaaaaac gagggcgctg ttgctgtggc cactgccatt aaagccggtg atgaacgtcg
     2821 cgcccgcgca aaatttcact ggcaattcct tgaacattat ccggctgctc aggactgcgc
     2881 ttataaattt cttgtctgtg aggataaacc cggtacacac cgcccagccc tcgactcctg
     2941 ggatgctgaa tatatgctgg aaaaccgctg ggatgaggag tctgcttcct ttgtcccggt
     3001 tgagactgaa tcagatccta tgaacgtcac ttttgacaag ctgtcccctg aagtacagaa
     3061 cgctgtcatg gttaagttcg atacatgtga aaacatcacc gttgatatgg tgattagcgc
     3121 acaggaattg ttgcaggaag acatggcaac cttcgacgga catatcgttg aagcgttgat
     3181 gaaaatgcca gaagttaacg ccatgtatcc ggagattaaa ctgcatgcca tcgggtgggt
     3241 taagcataaa tgtaagcctg gtgccaaatg gccagaaatt caggcagaga tgcgcatctg
     3301 gaaaaaacgt cgcgaaggtg aacgcaagga aaccggaaaa tacacgtctg ttgttgatct
     3361 cgcccgcgcc aaagccaatc aacagtacac tgaaaattca acaggaaaaa tcagcccggt
     3421 cattgctgcc attcatcgcg aatacaggca gacatggaaa acactggatg acgaactggc
     3481 ctacgctctc tggcctggtg atgtggatgc cggaaacatt gacggcaaca tccatcgctg
     3541 ggcaaaaaaa gaagttatcg acaacgaccg cgaagactgg aagcgtatct cggcatcaat
     3601 gcgcaaacaa cctgatgcac ttctatacag ccgtcagact attttcggcc ttgttcgtga
     3661 acgtccgatc gacattcata aagatcccgt ggcgctgaac aaatacatca ctgaatacct
     3721 gactacaaag ggcgtatttg aacatgaaga aacagaccag agccctgctg atgctctcca
     3781 cctgtcagca gaacaaactg attcagtgga gacggcagaa tccgatactc aaaaaaatga
     3841 aatcctggtg gaagctgaac catctgtaga gcgtgaaggg cctttttatt tcgtctttac
     3901 cgataaggac ggggaaaaat acggtcgcgc aaacaaactt tctggtctgg acaaggcgct
     3961 ggctgccggg gctactgaaa tcacgaaaga agaatatttt gcccgcaaaa acggcacata
     4021 cacgggctta ccgcaaaata cagatatatc tgaacattca gaacaaccag agccggtaaa
     4081 agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc agcctgactc
     4141 cgataagttg cttgctgcat cacgtggtga gtttgttgaa gggattagcg acccgaatga
     4201 tccgaaatgg gtgaagggga ttgaaacctg cgattctgtg aaccagaacc agtcagaaac
     4261 ggaacagaac gaccaaaaag cggaacaaaa cagcccaaat gcgttacaag acgagccaga
     4321 aacgaaacaa tccgaaccag tagcgcaaca ggaagaggaa aaagtctgta ccgcctgcgg
     4381 tcagagcagt ggcggcaact gccctgattg tggcgcggtg atgggcaacg caacctacca
     4441 ggaaacactc gatgaagaaa atcaggctgc agctcaggag gaaatggaag gctctggcgg
     4501 cgatcactac cacaccacag ataatgaaag tggcgagaca gcaaatccct taattaaggt
     4561 gaacggtcat cgtgaaagca catccaccag caggttgtgg caccatctga tgattgacct
     4621 tgaaacaatg ggcaaaaatc ctgatgcccc aataaacgct ctggccggta agttttttga
     4681 tccggcaacc ggagagatgg ggccagaatt cagcaaaact atcgatctgg aaaccgcagg
     4741 cggagtcatc gatcgggaca ccattaagtg gtggctgaaa cagtcacggg aagcacaatc
     4801 agccattctg accgatgaaa tcccgttgga tgacgcactg ttgcaattac gggaatttat
     4861 cgacgaaaac tccggtgaat tttttgttca ggtctgggga aatggagcca acttcgacaa
     4921 cgtgatttta cgccgttcat atgaacggca ggagatcccc tgcccgtggc gttacaccaa
     4981 tgatcgtgat gtaagaacga ttgttgccct ggggctggtg atggatttcg atgctcgaag
     5041 tgtcatcaca tttgagggtg aacgccataa tgccctgcac gatgcgcgtt accaggcaaa
     5101 atacgtttca gctatctggc aaaaactgtt cccgaatcag gctgattttt aatgttcaac
     5161 ccatatcgcc gcccaccagc tatagtggcg gcggtcatgc tgtaaggcac gtgaccacat
     5221 gtacgaatta actctatcgc cagcagaaat tcaagagatc acgagatacg aacggtatac
     5281 aaaacagcaa caacagttaa ggttgcacgg tatcccattt gtacccggtc ctaaaaacga
     5341 accaatagtt cttcgcagtg atgtcccgcg cggactgact gcgataccga aagtatctga
     5401 actggtttct gctgaccccg attttgaggc gctgaacaat gggaagacca agaaaaaaca
     5461 aaaaagataa tgcactaccg ccacgtgtta gatcgaatgg ttacagttac gtatggaaac
     5521 ccgaaggaag cacaaaaact atagggctcg gaagagtgcg ggaaaccacc atagctaaag
     5581 tctggcagaa ttatgaactg gaaaaagcaa aacgccacaa cataatgacc gtcgctaaat
     5641 tatggcacat gtttatggat tcccccgcat ttacagaact ggcccccaga acccaaaaag
     5701 attatcggca acatcagagg gcgttgctgg cagtcttcgg aaaagtactg gctgataatg
     5761 taaaaattga gcaggtaaga attttcatgg ataaacgggg gattgagagc aagacccagg
     5821 caaaccatga actggcaagt ctcagtcgtg tatacgggtg gggatatgaa cgtggatatg
     5881 tgaagaataa cccatgcaaa ggagtcagga aatttacgct taaagcccgc actgtttaca
     5941 tcacagatga acagtatgcg gcgatatatg cggaagcaat accacagtta cgcattgcaa
     6001 tggagatatc ctatctttgt gcggcaagac tcggtgatgt gctcgagctg aaatggcagg
     6061 atattatgga taaagggatc tacattgagc aaaacaagac cggcaccaaa caaatcaagg
     6121 aatggtctcc acgattacga accgcgatcc agttagccag aaatgtatct tcaggcacat
     6181 gtgaatatgt gatcaacaca accaagggcg ggaaggtcat agctaaaacg ctaaacaact
     6241 ggtggaacca agctaaacac gcagccgaga aaaaatccgg cgtcccgttt gggtgcaatt
     6301 tccatgacat aaaagccaaa gggatatcag attacgaagg aagcagtcgc gacaaacaga
     6361 ttttcagtgg acataaaaca gaaaatcagg tgttgattta tgatcgtaaa acaaaaatca
     6421 cgccgacact ggatttgcca cttgtggtca gcaagtaggc aactcaggaa attttctggc
     6481 aaaaaaagac ttggtatact aactctgaaa gctccctaac agcctgattt ttctaggtgt
     6541 taagtttggt taatttttca gcagtttttt gtattgcatt gatttttatt tgtttttcat
     6601 cggtcttgaa aaccggcgac ccgaaagggt tccagagttc gaatctctgc gcttccgcca
     6661 aataagataa ggggttagct aaatgctaac ccctttttct tttgcctgtc gaaattctca
     6721 gggcgttata tttgcttaat gacctgataa tccgctgtta aacctgttcc agatcaaatg
     6781 cgtaaagatg ggtaaaactt ctgggtgccc ttacgcatta tcattatgct gcttaattaa
     6841 ttacatctgt catagagagt gactcaatgg atcgtattgt tagttcttca catgaccgta
     6901 catcactgct tagcacccat aaggtgctgc gtaataccta ttttctgctg agcctgacgc
     6961 tggccttttc ggcgattacc gcaactgcca gtacggtgct gatgctgcca tctccgggtc
     7021 tgattctgac gctggtgggt atgtatggtt tgatgttcct gacctataaa acggcgaata
     7081 agccgaccgg gattatctcc gcattcgcct ttaccggttt tctgggttat atcctcggac
     7141 ctattctgaa cacctatctg tctgccggaa tgggtgacgt aatcgctatg gcactgggcg
     7201 gaacggcgtt agtgttcttc tgctgctctg catatgtgct gaccacccgc aaagatatgt
     7261 cgttcctcgg cggtatgctg atggcgggta ttgtggtggt gctgattggt atggttgcta
     7321 atatcttcct gcaactgcct gctctgcatc tggcgatcag cgcggtcttc attctgatct
     7381 cctctggcgc tatcttgttt gaaaccagca acatcattca tggcggtgag acgaactata
     7441 ttcgtgccac ggttagcctg tatgtttcgc tgtacaacat cttcgtcagc ctgctgagca
     7501 ttctgggctt cgctagccgc gattaatctc acccgctaac actttcatag cctcgcttta
     7561 tgcggggctt tgttttttgt tacactgccg ccagtctgat taaggtagta ataattatgc
     7621 tgatcttcga aggtaaagag atagaaacgg ataccgaagg ctatctcaaa gaaagcagcc
     7681 agtggagtga gccgctggct gtggtgattg cagagaacga agggatttcg ctgtcgccag
     7741 aacactggga agtggtgcgt tttgtgcgtg atttctatct ggaattcaat acttctccgg
     7801 cgattcgtat gctggtaaaa gcgatggcga ataaatttgg cgaagaaaaa ggtaacagcc
     7861 gctatttata tcgactgttt ccgaaaggtc cggctaagca agccaccaaa attgctggcc
     7921 tgccaaaacc ggtaaaatgt atttaacgga tacgaaaatc ggctaattcc cccgagggat
     7981 gatgcggctc gctaagcacc cgttccacac gcgcagaacg cgggccgcca ctctttaacc
     8041 acagcattaa tttttccacc tgcccttctt caccgcaggc aaccacttcc acgctgccat
     8101 cgtcaagatt tttggcgtac ccggttaaac ccagtctttt cgcttcgtac tgtgtggtgt
     8161 agcgaaatcc tacgccctga actcgcccgt aaacccaggc aattatgcag actttcgaca
     8221 ttgctgctct ccttatcctt aatggacgcg ttgcaaatcc atggaaaact ccggacaatg
     8281 acgcccattt ctttgcatcg acagaaaagt aaattatgag tgtacgttta gtgttagcca
     8341 aagggcgcga aaaatcatta cttcgtcgcc atccgtgggt cttttccggg gccgttgccc
     8401 gcatggaagg taaagccagc ctcggtgaaa ccatcgatat tgttgatcat cagggaaaat
     8461 ggttagcacg cggcgcttat tcaccagctt cgcaaatccg ggcgcgcgtc tggacgtttg
     8521 acccgtctga gtctatcgac attgcttttt tttcccgccg tttgcaacaa gcacaaaaat
     8581 ggcgtgactg gctggcgcaa aaagatggcc tcgacagcta tcgtttaatc gccggagaat
     8641 ctgatggcct gccgggtatt actatcgatc gtttcggtaa ttttctggtg ctgcaactgc
     8701 tgagtgctgg cgcagaatat cagcgcgcgg cattaattag tgccctgcaa acgctgtacc
     8761 cggaatgtgc gatttacgat cgcagcgatg ttgcggtacg taaaaaagaa gggatggagc
     8821 tgacccaggg cctcgtcacc ggcgagttgc cacctgccct gctgccgatt gaagaacacg
     8881 gcatgaaact gctggtggat attcagcacg gacacaaaac gggctactac ctggaccagc
     8941 gtgatagccg cctggctacc cgccgctacg ttgaaaataa acgtgtgctg aactgtttct
     9001 cctataccgg tggtttcgcc gtatcggcac tgatgggcgg ttgcagccag gttgtcagcg
     9061 ttgatacctc ccaggaagcg ctggatattg cacggcagaa cgttgagctg aacaaactgg
     9121 atctgagcaa ggctgagttt gtccgtgatg atgtctttaa attgctgcgt acttatcgcg
     9181 atcgcggtga aaaatttgac gttatcgtga tggacccgcc gaagtttgtt gagaataaaa
     9241 gccagttgat gggcgcgtgt cgtggctata aagatatcaa catgctggcg attcagttgc
     9301 tgaatgaagg cggtattctc ctgactttct cctgttccgg tctgatgacc agcgatttat
     9361 ttcagaaaat catcgcggat gccgcaattg atgccggccg tgatgtacaa tttatagagc
     9421 agttccgtca ggcagccgat catccggtga tcgctaccta tccggaaggg ctatatctga
     9481 aagggtttgc ctgtcgcgtc atgtaacttg aaatgtggaa ttttaccctt atatcaaagg
     9541 taaagatttc ccgggaggtg actatgattg ccagcaaatt cggtatcggc cagcaggtcc
     9601 gccattccct gttaggttat ctcggagtgg tcgtggatat cgacccggtt tattcgctct
     9661 ctgaaccgtc gcctgatgag ctggcggtga atgacgagct tcgcgccgct ccgtggtatc
     9721 acgtggtgat ggaggacgat aacggcctac cggttcatac ctacctggct gaagcccagc
     9781 taagtagcga attgcaggat gaacatcctg aacagccatc catggatgag ctggcgcaga
     9841 ccatccgcaa acaactccag gccccgcgtc tgcgtaactg atccctgaac gaatccccgg
     9901 aaggtttttg ccttccgggg attttttatt tggccaggcc caggcgaggg atctcaatag
     9961 ccgggcagcg atccatcacc acattcaacc cggcatcccg tgccagtact gccgcttgct
    10021 cattaatcac ccccagttgc atccagaggg ttttcgcgcc aatggcaata gcttcctgtg
    10081 ccacgcccca cgccgcttct gaattacgga aaacatcgac catatcgacc ttttccggaa
    10141 cgtctgccag cgtgccatac ccctgctggc ccagcagcgt tttgccggca acttttggcg
    10201 aaaccggaat gacgtgatac ccctggtcga gtagatattt catcacgcga tagctgggac
    10261 gatcgggttt gtcgcttgcc cccaccagcg caatggtgtg agtggacgtt aaaatgcctg
    10321 caatatcggt ttctttcatt atttcctcca gactgtttaa caaagtgtac gacaaagccg
    10381 acatgccaac catgcagcta atacgaatga atgagagcaa aagcgggaaa tatgtctata
    10441 tgttagtaag tatgattaaa gaaaaatttt gacacattat tattcgggcg gtcttttgac
    10501 aggagctacc tatgaaaacc ggcatcgtga ccaccttgat tgcattatgt ttgccggtat
    10561 ccgtttttgc caccacgctg cggctttcaa ccgatgtcga tcttctggta ctcgacggca
    10621 agaaggtttc cagctctctg ctgcgcggtg cggacagcat tgaactggat aatggaccgc
    10681 atcagttagt gtttcgcgtt gagaagacga ttcatctttc caatagtgaa gaacggctgt
    10741 atatctcccc accgctggtg gtcagtttta atacccagct catcaaccag gtcaattttc
    10801 gcctgcctcg cctggagaat gagcgggaag ctaaccattt tgatgccgcg ccgcgccttg
    10861 aattgttgga tggcgatgcg acgccgattc cggtaaagct ggatattctc gccattacct
    10921 caacggcaaa aacgattgat tatgaggtcg aggttgaacg ctataacaaa tccgcgaaac
    10981 gcgcttcact accgcaattt gccacgatga tggcagatga cagtacgctg ctttcgggtg
    11041 tttccgagct ggatgctatt ccaccgcagt ctcaggtgct cacagaacaa cggctgaagt
    11101 attggtttaa actggctgac ccacaaacgc gaaatacttt cctgcagtgg gcggaaaaac
    11161 aaccatcttc ctgagatttt tgtgcctgtg cgcaggcttt ttcggtcttt atcttgcagc
    11221 gataagtgct tacagtaatc tgtaggaaag ttaactacgg atgtacatta tggaactgac
    11281 gactcgcact ttacctgcgc ggaaacatat tgcgctggtg gcacacgatc actgcaaaca
    11341 aatgctgatg agctgggtgg aacggcatca accgttactg gaacaacacg tactgtatgc
    11401 aacaggcact accggtaact tgatttcccg tgcaaccggc atgaacgtca acgcgatgtt
    11461 gagtggccca atggggggtg accagcaggt tggcgcattg atctcagaag ggaaaattga
    11521 tgtattgatt ttcttctggg atccactaaa tgccgtgccg cacgatcctg acgtgaaagc
    11581 cttgctgcgt ctggcgacgg tatggaacat tccggtcgcc accaacgtgg caacggcaga
    11641 cttcattatc cagtcgccgc atttcaacga cgcggtcgat attcttatcc ccgattatca
    11701 gcgttatctc gctgaccgtc tgaagtaata ttgcacaggt ggcaaacgcc acctgtttct
    11761 tacggttttc tcgccaccgg cacatccaga tttttcagta tttccacaaa gggagaggga
    11821 ttctctttgt taaacagtgc ccatacccga tggcgtgccc gggtcagcgc cacgtacatt
    11881 aaacgccgtt cttcagcgtc cgggaaatcc tcaaccggtg gcagtagcgc ctcttccata
    11941 atcgactccc gcgccgcagc cggaaaacca tcacttccct cctgcaagcc aacgatgatg
    12001 acgtaatccg cctgttgccc tttgctggca tgaatggtca taaagtcgat ttgcaacttc
    12061 ggccagcgtg ttgccgcttt ttccaggctg gcaggcctca tgtgatggta acgcgccagg
    12121 atcagaatgc gctcttccgg tttggcataa ccagagagct tatccagcaa agcgtcaagt
    12181 tgactctcat ccaataacgt gacggctttt ttgtctccat tggttaagct gtttagcggc
    12241 tttttcagct ggcctgggtt ctgctgaata aaccggtttg ccacctcacc gatacgactg
    12301 ttaaaacggt aagtcgtgtc taaatcacag cgatcgcctt caccaaagtt ttcatggaaa
    12361 gcggtggtga gcgacatttg cgcaccgctg aatcggtaaa tcgcctgcca gtcatcacca
    12421 acggcgaaca acgtcgtctg actgttttgc ttgcgtaatg ccgctaacaa cgctgcccgc
    12481 tgcggcgaga tatcctgaaa ttcatcaacc agaatatgct tccacgggct gataaagcga
    12541 cctttctcca gaatcacaat cgcctgatga ataaggcccg aaaaatcgac agcgttttct
    12601 gccttcagcg cacctttcca ggcttttaat aacggggcca tcaacttgat acgtttactg
    12661 aacagatcgc gaatctcttc gggtgcactg gcaatcattt ctgcctgtgc accaccgtgc
    12721 atccgcatca gacttaccca gcgatcgagg cgagaggcaa ggcgacgctg taatttttca
    12781 tcatcccaga agttaccttc tggcactgac cactgcattt cttccgtcag ccattgccgc
    12841 cagcccttct cctgcgcttt cttttcgctg cattgcttgc gccactcagc aataaagagt
    12901 tcatgacggg cagcggtatc attttccagt ttgctgacta tcggaacttt tttgctgccc
    12961 tgctgaataa tatgcagcgc cagcgcatga aacgtgcgtg cggtaatgtc ttcggtatgt
    13021 agccgttcgc gaatccgctc gtccatctct tcagcggctt tgcgaccaaa cgccagcaat
    13081 aaaatttgct caggggacgc ttcaccacgc gccagcaacc agcctgcacg ggccaccagc
    13141 accgacgttt ttccgcttcc tgcacctgcc agcactaaca gagaatgctc gccattaacg
    13201 actgcccggg cctgcgccgg attcagcggt gaagactcga cctggcggaa aaaatccgca
    13261 tactcggtaa gcatggcttc ggtatacgcc tggttatgct gcaaccgagc gctttcaata
    13321 tctttcaacc aggcctgaca tttacgccac gcctcacggc agttatcgaa ttcttccagt
    13381 cggttaaccg gcaacggcaa cgccgacaaa gcctggcgga tttgttgctg aacgccagag
    13441 gtttgctcac gcgtcagcca tttattttct ccagtgcgcg tggcaatcaa atccagttgc
    13501 tggcgtaaaa caccagacgc aatttcgctc atctcgccac tccaccgccg ccagtgagca
    13561 tcaagatgat ggtaaaaacg ctgcgtctcg ccccattcgg tgccgtgcag acgcaccact
    13621 ttttcgtccg gcagtacaaa ttccagctcg ccccatacca gaccgcgctt acagtgaatc
    13681 gccagtaatt gattgaaagg aataaggtat tcgtggcgat cgccggagac tttaatcccg
    13741 gcattgagga tcaccgcccg atcgtaaggg tgctgtgcca gacgttttcc aagcgttgtc
    13801 gctttcagtt ccatgactct gtagatccac acgaaattat ttcttatcag tgtaaccgtc
    13861 agagaaaacg tgctccagcc caaaaaaacg ttacaattgc cgcccattac tgaaaaccac
    13921 agtaaagcga ggttttatgc gtaccgtttt gaacattctg aactttgtgc ttggcggatt
    13981 tgccaccact ctgggctggc tgttggcgac tctggtcagt attgtgctga tttttacctt
    14041 accgctgaca cgatcctgct gggagatcac taaactgtct ctggtgcctt atggcaatga
    14101 agctattcat gtcgatgaac tgaacccggc tggcaaaaat gtactgctga atactggcgg
    14161 tacggtattg aatattttct ggctgatttt ctttggctgg tggttatgcc tgatgcacat
    14221 tgcaacgggc atcgcacaat gtatttcaat cattggcatt cctgtcggca ttgcgaactt
    14281 taaaattgcc gctattgcac tatggccggt tggtcgtcgc gtggtatcgg tagaaacggc
    14341 gcaagctgcg cgtgaagcca atgcacgtcg tcgttttgaa taatccacta atatggcctt
    14401 tatgctaagt cctttgctca aacgctatac ctggaacagc gcctggctgt attacgcgcg
    14461 tatttttatt gcgctttgtg gaaccacagc gtttccgtgg tggctgggtg atgtaaaact
    14521 gacgattccg ctaacgctgg ggatggtggc agcggcgctg accgatctcg atgaccgact
    14581 ggcgggacgt ttgcgtaacc tcatcattac gctgttctgc ttttttatcg cctcggcctc
    14641 agtagaattg ctgtttccct ggccctggct atttgcgatt ggcttaacgc tctctaccag
    14701 cggcttcatt ttgctcggcg gtctgggtca acgctatgca acaattgcct tcggtgcatt
    14761 gctgatcgcc atttacacta tgttgggaac atcactttat gagcactggt atcagcagcc
    14821 gatttatctg ctggccggtg ccgtctggta caacgtcctg acacttattg gtcatctgct
    14881 gttcccggtc cgcccgctgc aggacaacct ggcgcgttgc tatgaacaac tggcgcgtta
    14941 tcttgagctc aagtcgcgca tgtttgatcc tgatattgaa gatgaaagcc aggcaccgct
    15001 gtacgatttg gctctcgcca acggacaact gatggcgaca ttgaatcaga cgaaactctc
    15061 gctgctgacc cgcttacgtg gcgatcgtgg tcaacgggga acgcgtcgca cgctgcatta
    15121 ttactttgtc gcgcaggata ttcacgagcg tgccagctct tctcatattc agtatcaaac
    15181 attgcgtgaa cattttcgcc acagcgacgt gctgttccgt tttcagcggc tgatgtcgat
    15241 gcagggccag gcgtgccagc aactgtcacg ctgtattttg ctgcgtcagc cttatcaaca
    15301 tgatccgcat tttgagcgcg cttttacgca tattgatgct gcgctggagc ggatgcgcga
    15361 taacggcgca cccgccgatt tactcaaaac actgggattt ttgctgaaca atttacgcgc
    15421 cattgatgcc caactggcaa caattgaatc agaacaggcc caggcactac cccataataa
    15481 tgacgaaaat gagctcgctg atgacagccc gcacgggttg agtgatatct ggctgcgtct
    15541 tagccgtcac ttcacgccgg aatccgccct cttccgtcat gcggtaagaa tgtcgctggt
    15601 gttgtgcttc ggctacgcca tcattcaaat aaccggaatg catcacgggt attggatctt
    15661 gctgacaagt ttgtttgtct gccagccaaa ctataacgcc acgcgccacc gcctgaagtt
    15721 aaggattatt ggtacgctgg taggtatcgc cattggcatt cctgtgctgt ggtttgtgcc
    15781 atcactggaa gggcagctgg tgctgctggt tattaccggc gtgctctttt ttgccttccg
    15841 taacgtgcaa tacgctcatg caacgatgtt catcacactt ttggtgctac tgtgttttaa
    15901 cttactgggt gaaggttttg aagtagcgtt acctcgcgta atcgatacgc tgattggttg
    15961 tgccattgcg tgggcggcag tgagctacat ctggcctgac tggcagtttc gcaatctgcc
    16021 gcgaatgctc gaacgcgcca cagaggccaa ctgccggtat ctcgatgcca tactggagca
    16081 ataccatcag gggcgtgata accgtctggc gtatcgtatt gcccgccgcg atgcacacaa
    16141 ccgtgatgct gaactggcgt cggtggtatc aaatatgtcc agcgaaccga acgttacccc
    16201 gcaaattcgc gaagccgcgt ttcggttgct gtgccttaac catacgttta ccagctatat
    16261 ctcagccctc ggtgctcacc gggagcagtt aactaatcct gaaattctgg cgtttcttga
    16321 tgacgcagtt tgctatgttg atgacgcgtt acatcatcaa cctgctgatg aagaacgcgt
    16381 caatgaggca ttagctagcc tgaaacagcg gatgcagcaa cttgaaccac gggcagacag
    16441 caaagaacct ctggtcgtac aacaagttgg attattgatt gcattactgc ctgagattgg
    16501 tcgtctgcaa cgccagatta ctcaagttcc gcaggaaact cctgtttcgg cgtaagagag
    16561 tcagcccatt ctgcaagctc ctggcggcgt gccaccggga gcgcagcttc atgaatgccg
    16621 ataatggcac cttcaagcat aaacagaatc ttttctgtca ccagactgtt ttgctgccgc
    16681 agtcgcaacc aacacatttt tgccccaagt atacgtaacg cccgtacatc cttaatcccc
    16741 acctccccga gaatcgcttc cagatgaaaa gacatattgg gcaaatcttt cagtctttcc
    16801 cgggtattgc gcgtgctttt ctctttcagc gctgcatcga gagaatattt cgacagacgc
    16861 accagcttca gttgatttcg ccatagactt tcatcaaccc gatagtaatt gagggtaacg
    16921 gatcggccac actttttata tgtcagccag acaggcggat gttttacaca gtactgtgca
    16981 ctttgctcac aagcccgaag atacaactca ccatcagaaa ccatcgcaaa caccgtgtcg
    17041 tcaacggtca ggctgtaact gccaaacaat gatcggtatt caattgtgcc caacgttgcc
    17101 aggtattctt gtgatttata gatccgctta taggagaggc ttttcataaa attcctttta
    17161 aaatcataac ataaaagaat gattcacatt aacggatccg ttaactacga aaataggcaa
    17221 cttattctta aggggcaaga ttaatttatg ttttcccgtc accaacgaca aaatttgcga
    17281 ggctctttcc gaaaataggg ttgatctttg ttgtcactgg atgtactgta tatccataca
    17341 gtaactcaca ggggctggat tgattatgta cacttcaggc tatgcacatc gttcttcgtc
    17401 gttctcatcc gcagcaagta aaattgcgcg tgtctctacg gaaaacacta cagccgggct
    17461 tatcagtgaa gttgtctatc gcgaagatca gcccatgatg acgcaacttc tactgttgcc
    17521 attgttacag caactcggtc agcaatcgcg ctggcaactc tggttaacac cgcaacaaaa
    17581 actgagtcgg gaatgggttc aggcatctgg gctaccctta acgaaagtaa tgcagattag
    17641 ccagctctcc ccttgccaca ctgtggagtc aatggttcgc gctttacgca cgggcaatta
    17701 cagtgtggtg atcggttggt tggcagatga tttgactgaa gaagagcatg ctgaacttgt
    17761 tgatgcggca aatgaaggta acgctatggg gtttattatg cgtccggtaa gcgcatcctc
    17821 tcacgccacg agacaacttt ccgggctaaa aattcactct aatttgtatc attaagtaaa
    17881 tttaggatta atcctggaac tttttttgtc gcccagccaa tgctttcagt cgtgactaat
    17941 tttccttgcg gaggcttgtc tgaagcggtt tccgcgattt tcttctgtaa attgtcgctg
    18001 acaaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
    18061 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
    18121 cgaatatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
    18181 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
    18241 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
    18301 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
    18361 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
    18421 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
    18481 tacaaaggcg acaacatcaa cggtgcatac aaagctcagg gcgttcaact gactgctaaa
    18541 ctgggttacc caatcactga cgatctggac gtatacactc gtctgggtgg tatggtatgg
    18601 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
    18661 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
    18721 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
    18781 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
    18841 gttgctccgg ctccagctcc ggcaccggaa gtacagacca agcacttcac tctgaagtct
    18901 gacgttctgt tcaacttcaa caaagcaacc ctgaaaccgg aaggtcaggc tgctctggat
    18961 cagctgtaca gccagctgag caacctggat ccgaaagacg gttccgtagt tgttctgggt
    19021 tacactgacc gcatcggttc tgacgcttac aaccagggtc tgtccgagcg ccgtgctcag
    19081 tctgttgttg attacctgat ctccaaaggt atcccggcag acaagatctc cgcacgtggt
    19141 atgggcgaat ccaacccggt tactggcaac acctgtgaca acgtgaaaca gcgtgctgca
    19201 ctgatcgact gcctggctcc ggatcgtcgc gtagagatcg aagttaaagg catcaaagac
    19261 gttgtaactc agccgcaggc ttaagttctc gtctggtaga aaaaccccgc agctgcgggg
    19321 tttttttcgc ctttagtaaa ttgaactgac ttcgtcagtt attccttacc cagcaatgcc
    19381 tgcagatcat gcttcaaaga agacatttta ttcgcgtatt tctctttgtt ttccgcatct
    19441 tcaatcagct gaacaatcgt ttcagaaagc gttttaccgc gacgctgcgc aagaccagcc
    19501 agacgttgcc agacaataaa ttccagatcg atcgattttt tgcgcgtatg ctggtgttcc
    19561 gcattaaaat ggcgcttccg tctggcccga atggtttgct tcatgcgatt gaccagttcc
    19621 ggattcatat gcttgtcaat ccagccattt accagcacgg gttcattttc cagcgagagc
    19681 aacacatcga cggcttcctg ggcggcactg gcttctatgt aacgggtgat taactcccct
    19741 tcgcgatgct tcttcaccag atacttccat ttccaaccgc tttcaagatt ttcaagttgt
    19801 tgatatttca tagcgatctc aatgttaccg tgtaactctt tacagaatat cagcttttta
    19861 ggccagtgaa gaaaagaatc tccatcctgt gagctttaac gcccatgcca ggagtattgt
    19921 cggtgctttc tacgtgtgct gtagtgccgg ttacggtata atcgcggctt tgacaacaga
    19981 ctaaaaaaca tcaactttga ccattacgaa acttgcatgg cgtgacctgg ttcctgatac
    20041 cgatagctat caggaaatat ttgctcagcc acatttgatt gacgaaaacg atcctttatt
    20101 cagtgatact caaccgcgac tgcaattcgc actggagcag ttgctgcata cgcgagcatc
    20161 ctcctctttt atgctggcga aggccccgga agagtctgag tatctgaatc ttattgccaa
    20221 tgccgcgcgt acgctacaaa gcgatgcagg ccaactggtg ggcggtcact atgaggtttc
    20281 cggccactcc atccgcttac gtcacgcagt gagtgcagat gataattttg cgactttaac
    20341 gcaagttgtc gctgccgact gggtagaagc ggagcaactc tttggctgcc tgcgccagtt
    20401 taatggcgac attaccctgc agcctggtct ggtgcatcag gcaaatggcg gtattctcat
    20461 catctctttg cgtacactgc tggcgcaacc tctgctgtgg atgcggctga aaaatatcgt
    20521 taaccgcgag cgttttgact gggttgcgtt tgatgagtcg cgccctctcc ccgtctctgt
    20581 gccttcgatg ccattgaagc tgaaagtcat tctggtaggc gaacgcgaat cattggctga
    20641 tttccaggag atggagccag agctttcaga gcaggctatt tatagcgaat ttgaagatac
    20701 tctgcagatt gtcgatgcgg agtcagtaac ccagtggtgt cgctgggtga catttaccgc
    20761 cagacataat cacttacctg caccgggagc ggatgcctgg ccggtactta tccgcgaagc
    20821 agcacgctac accggtgaac aagaaacact tccgcttagc ccgcagtgga tcctccgcca
    20881 gtgtaaagag gtcgcctccc tgtgtgatgg cgacaccttc tccggcgagc agctaaactt
    20941 aatgctgcag cagcgtgaat ggcgcgaagg tttcctcgct gaacgtatgc aggatgagat
    21001 ccttcaggag caaatcctga ttgaaaccga aggcgaacgc atcgggcaaa ttaacgccct
    21061 ttcggtcatt gaatttccgg gtcatccacg cgcttttggc gaaccttctc gcattagctg
    21121 cgttgtgcat attggcgatg gtgaattcac cgacatcgaa cgcaaagcgg agcttggcgg
    21181 caatatccat gcgaaaggga tgatgatcat gcaagcgttc ctgatgtcgg aactacagct
    21241 tgagcaacag atccccttct cagcatcgct gacatttgag cagtcataca gtgaagttga
    21301 tggagatagt gcctcgatgg ctgaactctg cgccctgata agcgccctcg ccgatgtgcc
    21361 ggtgaatcag agtatcgcta tcacaggttc agtcgatcag ttcggtcgcg cccagccggt
    21421 cggtggttta aatgagaaaa tcgaaggctt ctttgctatt tgccagcaac gtgagttaac
    21481 cgggaaacaa ggtgtcatta tccccacagc taacgttcgc catttaagtc ttcacagtga
    21541 actggtgaaa gcggtagaag aaggcaaatt caccatctgg gcagtagacg atgtgactga
    21601 cgcactgccg ttattattaa atctggtgtg ggatggcgaa ggccaaacga cgctgatgca
    21661 aaccatccag gaacgtatcg cgcaagcatc gcaacaggaa ggacgtcacc gttttccatg
    21721 gccattacgt tggctgaact ggtttattcc gaactgatcg gacttgttca gcgtacacgt
    21781 gttagctatc ctgcgtgctt caataaaata aggcttacag agaacatggt agataaacgc
    21841 gaatcctata caaaagaaga ccttcttgcc tctggtcgcg gtgaactgtt tggcgctaaa
    21901 ggcccgcaat tgccagcacc gaacatgctg atgatggacc gtgtggtcaa aatgaccgaa
    21961 acgggtggta acttcgacaa agggtatgtt gaagcagaac tggatatcaa tccggatctg
    22021 tggttcttcg gatgccactt tattggcgat ccggttatgc cgggatgcct gggcctggac
    22081 gcaatgtggc agctggtagg gttctacctc ggctggctgg gcggcgaagg taaaggccgc
    22141 gcgctgggcg ttggcgaagt gaaattcact ggtcaggtac tgccgacagc gaaaaaagtg
    22201 acctaccgta ttcactttaa acgcattgtt aaccgtcgtc tgattatggg cctggcggat
    22261 ggcgaagtgc tggttgatgg tcgtcttatc tataccgcca gcgacctgaa agtcggtctg
    22321 ttccaggata cgtctgcctt ctgattataa tcagacagag tatcaaaagg cgaaacctcc
    22381 gcaatgcgga ggtttctttt taaagagaca gaatcaggcc attactaccc tgtccgccat
    22441 ggcttctcgc cagcctccca gccattgtga cctttgattc agcgtctgat agggacacat
    22501 ttcttttgag cgtccggcga tgccggcctg ataaccacgt tgatgtgccc gttccaggcg
    22561 atctcgtttt tgtctcttca tgcctcgttt ccctcatact ggtttctggt ggaaaagaaa
    22621 acagtgatta ctaaatgtgc aatcacagtt aagcaatacc gcgaatcata actgccgtca
    22681 atgcgcaaaa ttcacgccac tgtcatattt gtgagctaca cgggcatgtc ggttgtacaa
    22741 aaaatgtgaa gaggaacaaa tttttccgca cgttagaaac aaaaaaaccg cctcaggcaa
    22801 cataccggag acggttatat ttacaaatct ttataattaa ggtagacgct ttatctcttg
    22861 cgcaatagaa gcggcttcct gactccagac actggccagt actttaacca tctcatcgta
    22921 accatcctga gtttgcactc cttccagacg gaacggacgt ttgatcagtt gtccctggtg
    22981 gttcaacagc cactcaccac tgacaatgac cttgccatca tagcgaccgt taaactccgt
    23041 tacggtaaca ttgagcgtgt cctgggcgct tcccagaggc tgggaggcaa ccacccagcc
    23101 gggcagttgc gtactcaggt tggcaaccag ggtgttgcgc aactgttgat ccaacgggct
    23161 ggcccacaag ttgttgttgg caatcacata cttcacatca ctggtttgat aaaccacacc
    23221 attccccgcc agatagtcag gaacagtgac ctgctctacc cataacagac gattgccctg
    23281 gctggcggta ctttgtgtac cgctctgcac cacaggtaac tggtaatagt ttttattaat
    23341 ttcgccggag ctgcatccga ccagccacag tgctgcaatc gtcactagcc actttttcat
    23401 tgtttcgccc tcttcggctc tggatctttt ttgtccttcg cttcaaatac cagcgcgtta
    23461 ctcttctcat tgagcgtttt cagcaccggt tgcagttctc gcaacacctg atcaaggcgc
    23521 tgcatatccg ccaccatctt gttgtaggct gcggagccag gctggaagcc ctgcatgctg
    23581 cgattcaatt cacgcaacgt tgactgcata tccgtcggca actgctgcat cgactggcta
    23641 gcgaggatct tgttcatgct atccagcgtc gtttgcaggt ttttcattgt gcgctgactt
    23701 tcagaaagcg tactggttgc ctgttcaatc atcggattca atggcagttt gttgatctta
    23761 tccaacgctt ccatcagtcg ttgctggatt tgcgccaggc cgccgctaac ggtcgggata
    23821 atctgataac cattaaattc acgaataccg gttattgcag gcgtatttgg atagaaatcg
    23881 agatcaacat acagtgcacc agtgaccagg tttccggttt tcagcgatcc gcgtaaacca
    23941 cgtttcaaca attcgccaag gtgctcaaca acatccgcat tttcgccaag ctgcattttc
    24001 agccgctctg gctcgatacg aatcagtacc ggaatacggt aatcatcgtt aaatgtctga
    24061 cgcatattcg gcgcaaagaa tggcactttg cttacggtac ccaggcgaat accccggaac
    24121 tctaccggag ctcccggttg cagaccgcgt accgaatctt taaagaacat cagataatca
    24181 atgtgatcgg tgtacaacga atcctgaatg ctcttctgat catcatacaa aacgaaagct
    24241 gttttcggtg ccactggctg ccctaaatcc agaccttccg gcacatcaaa gctgacaccg
    24301 ccactcagca gcgttgtcaa tgagcccatc tccacacgca tccctgctga cgtcagatca
    24361 accgcaatgc cactatcttt ccagaagcga acattgttgg tcaccagtcg gtcataaggt
    24421 gcattgatga acagttgata gctgatattg cgtttttgtg tatcgaaggt gctggtttca
    24481 accgaaccta cccgatagcc acggaacagc accggatctc ctggcgagag ctgcccggct
    24541 tttttgctat cgagaatcac acggatacct ttcgcatcag gcggggccaa cggtggcgag
    24601 tccagcaaat cgtatttatc cattttgctg cctttcgcgc ctggctgcag ttcgatataa
    24661 actccagaca gcagcgttcc caggccgcta atcccttcgc gaccaatctg cggtttcacc
    24721 acccaaaaga cggtgtcttt atgcagcaat ttttccatac cggaattcag ccgcgctttg
    24781 atttcaacgt gcgtcaaatc atcagccagt gtggcgcttt caaccacgcc gacgtcaacg
    24841 ctacggcttt taatggtggt tttgccacct tcaattcctt ccgcattcgc ggtgatcagg
    24901 gtcacttccg gtccctgatg gctgtaatga taaaaaagaa cccaggcccc aatgagcgcc
    24961 gtgacgatag gaaatatcca cacgggagac cagttcttca ctttctggat tttggcttcc
    25021 ccattattag attccatact gtcaggactc ctcatgctct gattcaggtt gacgatccca
    25081 cgacaaacgc gggtcaaacg tcatagcaga aaacattgtc attatgacga ctaaagcaaa
    25141 cattaatgca cccattgccg gataaatact cattaaacct cccatacgca ccagcgccga
    25201 gagcaccgcg ataacgaaaa cgtcaatcat cgaccagcgg cctacaaact caacaacttc
    25261 ataaatcaaa tgcattcttt cactgtcgcg cttgccatgc cctttggcat cccaacacag
    25321 ccacgcgatg gcgatcatct ttaacgttgg taccataata ctggccagaa agatcaccgc
    25381 agcgacggga taagagcctt cgctccataa cagaatgacc ccagcgagaa tcgtcgacgg
    25441 catcttcgag cctaataaat ccgtcaccat gatgggcaaa atattagccg gaagatacag
    25501 catgatggac gttacaagca gcgcgagtgt ccactgcagg ctgtttctac gccgaacgta
    25561 ccctttggta ctacaacgcg ggcacacggg ttcatcagca ggaaggattg ccgtacaaca
    25621 ggagcacgaa cgcagcccct gacgtatccc cgtgacgcct ggttttagcg gctggcgcag
    25681 ttctggcatc ggggcgatgt cgtcccataa ccagcgacga tcaacgcact gaaaagcgcg
    25741 cagttgcagg acacaaaata aacaccaggg gagaaagctg ctgcctaccc caatgctgcc
    25801 gtaagccatc agtttaacga aactgaccag cacaccagcg aggaaaatct ccgccattcc
    25861 ccaggttttg agttgaaaaa gcacccgtgc cagttgctct tttaaacgga ccggtaattc
    25921 cgcgcgattc accagtaaca gaatggttat cagacaaaac gcgggaacca gttgcacaaa
    25981 caacaggaaa aaggtgccga ggctggcgta gtcctcagaa aaaagcacgc cgggaatttc
    26041 cagtaatgta atttcactgg taactcctgc aacgttcata ttcacaaaag gaaacaagtt
    26101 ggacagcaac agcatgaaca gtgcagccaa cgcataggcg gtgggacgct gccgaggggc
    26161 atcccacgcc acggttaacg ttgtgccaca ccggggacat gccgctttct gaccatgctc
    26221 aaggcgcggt aacgccacca gcatgtcaca ctgcgagcac aggatgtgct tcgcggcatg
    26281 atgatgttcg cacattggta ctccttaatg atgcgctatt ttacagatga ctaacaaagt
    26341 gttggttttc atgcctgata aacccagttc atcaggcaat tttaccgttg tcagctgtct
    26401 ctgtttaaat cgactatttt gcgatcagcc accatttttt aacgcttcaa gatactccca
    26461 gcgttcaaag gcttgctcca gctcctgctc tgcagcagcc atatcagcaa gcactttttg
    26521 cgtctgctca tgcggctgac tgaagaagga agcatccgcc acttgcgtct gtagggcttc
    26581 cagcttcgcc tccagatctt cgagcaattg cggtagctgc tccagttcgc gctgcaattt
    26641 atagcttagt ttgctactgc tgcgttttac agtttctgct tttgccgcgg cggcttcttc
    26701 ggtttttttc accgcaggct gtttgagcgc cacatactgc tcttgctgac cacgggcatc
    26761 atgataaccg ccgacataac gaccaatttt accgccgcct tcgaagatcc aacattctgt
    26821 aacggtgtta tcgacaaact gacgatcgtg gctaaccagc aataccgtgc cctgatagct
    26881 gtcgatcagt tcttccagca gttccagcgt ttcgacatca agatcgttgg tcggttcgtc
    26941 aagaatcaat aagttgcttg gtttgaggaa caaacgcgcc agcagcaagc ggttccgctc
    27001 accgccagaa agcgcacgta ccggcgtcat cgcccgtttc ggatggaaca gaaagtcctg
    27061 caaatagccc aatacgtggc gtggcttgcc gttaaccatc acctcttgct taccttcggc
    27121 aaggttatcc atcaccgttt tatcgggatc cagttccgcg cggtgctgat cgaaataagc
    27181 cacttccagt ttggtgccaa cgtgaatacg cccgctgtcc gcttgaagct gaccgagcat
    27241 cagtttcagc agcgtggttt tgccgcaccc attcggacca atcagggcaa ttttgtcgcc
    27301 acgtagaacc tgggccgaaa aatctttcac cagttgctta ccgtcaacct ggtagcaaac
    27361 gtcttccatt tcgaaaacga tcttaccgga gcggctggcc tcttccacct gcatctttgc
    27421 ggtacccatc acttcgcgac gttcaccacg ctcgcgacgc atcgctttca gggcgcgtac
    27481 gcggccttca ttacgggtac ggcgtgcttt gatcccctgg cggatccaca cctcttcctg
    27541 cgccagtttg cgatcgaact cggcattttg taattcttcc acgcgcaggg cttcttcttt
    27601 ttccagcagg tactggtcgt aattccctgg ataggtcacc agcttgccgc gatcgagatc
    27661 aacaatgcgc gtcgccatat tgcggataaa cgaacggtcg tgggagatga aaataatcgt
    27721 cccgttgaaa gttttcaaaa acccttccag ccagtcgatg gtttcaatat ccaggtggtt
    27781 tgtcggttca tcaagcaaca gcacgcgcgg attactcacc agcgcgcgtc ctaatgccgc
    27841 tttacgcaac cagccgccgg aaagcgacga cagcgcaacg ttaggatcta accccagttg
    27901 cgccagcact tcgttgatgc ggttttccag ctgccacagg ttgtggtgat ccagctgttc
    27961 ctgaaccttc gccagttcgt tgagattttt ctcgctcggg tcgttcatca ccaggcgcga
    28021 aatatcgtga tagcgtttca gatattccgc ttgttcttca atgccttcgg caacgaaatc
    28081 ataaacgcta ccctcaacgt tacgcggcgg atcctgttgc agacgagcta caatcaaatc
    28141 ttgctcgtaa ataatgcgac cgtcatccag cccttgttca cggttgagga ttttcattaa
    28201 cgtcgatttg cccgcgccgt tgcggcccac cagacaaaca cgttcgttat cttcgatatg
    28261 cagttctgcg ttatcgagaa gcggcgcgtc gctgaacgac agccatgcgc catgcatact
    28321 gattaatgac attactattc ctttcaggct gcggtaatca gccagcagtt gtggatctgg
    28381 cggttacggg cgaaatcctg ggagagcgtt ttttgcgtaa tttcttgtgc tttcagtccc
    28441 agttttgcca ggccgtcgag atccatacgg aagccacgtt tattgttcga gaacatgatc
    28501 gtcccacctg cacgcagcag acgtttcaaa tctttcatca gcgccagatg atcgcgctga
    28561 acatcaaacg catcttccat tcgttttgag ttagagaagg ttggcggatc gataaagatc
    28621 agatcgaact gttcatttgc ctcacgcagc cacgccaggc aatcggcctg aatcaggcga
    28681 tgcgcacgcc cggtcaggcc attcaaacgc aggttgcgtt ctgcccactc cagataagta
    28741 cgcgacatat ccacggtggt ggtgctgcgt gcaccgccta atcccgcatg cacggtggcg
    28801 ctgccggtat aagagaacag gttgaggaaa tctttgcctt tgctcatctg accgagcata
    28861 cgacgggcga tacggtgatc gagaaacaga ccggtatcga ggtaatccgt caggttcacc
    28921 aacaagtgag cgttatattc ggtaacttca agaaactcgc ccttctcgcc cagtttctgg
    28981 tactgatttt tgcccttctg gcgttcacgg gttttcagca ccagtttgtt tggcgcaatc
    29041 cccagtaccg aaatggttgc agcgataata tcgaacagac gctgacgcgc tttgtgagca
    29101 tcaatagttt ttggcggcgc atactcctgc accaccaccc agtcggcata acggtcaacg
    29161 gcaacgttat attccggtag atcggcgtca tacaggcggt aacattcaat tccttcctgg
    29221 cgagcccact tctcgaattt tttgaggttc ttacgcaggc ggttggcgta gtcttccgct
    29281 accatcgccg gtttgctgtc tggagtgctt tcggcaacgt ggtaattttt ctgtacgcag
    29341 tccagcgggc cgtttttcgc cttgtactgt ttgtctgcac gcagctgcaa gcagcttagc
    29401 agatccggcg aggcactaaa caaagagaga ttccagccac caaactggtt tttcatgatc
    29461 cgacccagca ggctatgcag cgcaatcagc gccggttcgc tgtccagacg ttccccgtat
    29521 ggcgggttgc tcaacactgt gccgtacggc cctttcggca gcggattggt cagttgcgcg
    29581 acatctttca cctcaaaggt gatcagttca ccaatccccg caagacgggc gttagtgcgc
    29641 gcacgttgaa tcacccgtgc gtcgctgtcc gaaccgtaaa aatgagagct atactcagcc
    29701 aggcctttac gggcacgagt ttgcgcttcc gctttcactt cctgccagat agcttcatca
    29761 tgctgcaccc agccgctaaa gccccaacgc ccacggtgca agcctggtgc gcgatcggtc
    29821 gctagcatcg ccgcttcaat cagcaacgta ccggaaccac acatcggatc gagcagcggt
    29881 gttcctggct gccagccgga tcgcatcaca atagcggctg ccagggtttc tttgatcggc
    29941 gcaataccag cacgatcgcg atagccacgc agatgtaaac catcaccact cagatcaaga
    30001 gcgatactgg cggtttcttt atgcagccag acgttaacgc ggatatccgg cgcatcgcga
    30061 tcaacatttg gacgcggcag atttttccgc gtgaaagcat cgacgatcgc gtctttcact
    30121 ttcatcgcgc cgtactgact gttgcggatg gtgtcattca aaccactgaa gtggacagcg
    30181 aaggtcgcgc caggattaaa catctctgtc cagttgatcg cctgaacacc gagatagagg
    30241 tctaaatcgc tgtaaacctt acactcgccc agcggcaaca taatacgcga ggccaggcgg
    30301 ctccacatca ggctctggta aacaagccgt gtgtcgccct tgaaatggac cccaccctga
    30361 accacctggc attcaacggc ccccaggttt tccagttcag tttttaacag ctcttccagc
    30421 ccacgggccg tactggcaaa cagagaattc atatcgtcac ttttactcta agagcctacc
    30481 gcagtaggcg taattgttgc agtcagtctg gacatggaca gcgcggagaa accggagcgt
    30541 acacaagtac gtgaggattt cgagcacaac ccagggccaa actgacaaat aaaatagcct
    30601 aatgggacag gcgctaagaa aattgttgcg cattatagct aataagcgcg tcatgtcata
    30661 aagttgaggg cttattttca tttgaggacc gcaccgtggt gacattaacc cggcttttta
    30721 ttcatcctgt taaatcgatg cgcggcattg gtcttacaca tgctctggct gatgtcagtg
    30781 gtctggcctt cgatcgcatc tttatgatca cggaacctga cggtacgttt attaccgctc
    30841 gccagtttcc ccagatggta cggtttactc cttcacctgt gcatgatggc ttgcatctca
    30901 ccgcaccaga tggcagtagc gcatatgttc gttttgctga tttcgccaca caagacgcac
    30961 caaccgaagt ttggggcaca cattttaccg cgcgaattgc gccagacgcg atcaacaaat
    31021 ggctaagtgg atttttctcc cgcgaagtgc aattacgctg ggtggggcca caaatgaccc
    31081 ggcgcgtgaa acgccacaac actgtacccc tgtcatttgc tgatggctat ccttaccttc
    31141 ttgctaacga agcctcgtta cgtgatctcc aacaacgttg tccggccagt gtaaaaatgg
    31201 agcaattccg ccccaatctg gtggtttccg gcgcgtcagc ctgggaagaa gatagctgga
    31261 aagtgattcg cattggtgat gtggtgtttg atgtggttaa accttgtagc cgctgtattt
    31321 tcaccaccgt cagcccagaa aaagggcaaa aacatccggc aggcgaacca ttaaaaacat
    31381 tgcaatcttt ccgcactgcc caggataacg gcgatgtcga ttttggtcag aatttaattg
    31441 cccgtaatag cggcgtgatt cgcgttggcg atgaggtgga aattctggca acggctccgg
    31501 caaaaattta cggcgcagct gccgctgatg ataccgccaa catcacgcaa caaccggacg
    31561 caaatgtaga tattgactgg cagggacagg catttcgtgg aaataaccaa caggtgttgc
    31621 tggagcaatt agaaaatcag ggaattcgta tcccttattc ttgccgcgcg ggcatttgtg
    31681 gaagttgccg tgttcagctt ttagaaggcg aagtcacgcc gctgaaaaaa tcagcaatgg
    31741 gcgatgatgg caccattctt tgctgtagct gtgtaccgaa gactgcactt aagttggcgc
    31801 gttagacagc ctgttcgagg ctgaagctgt caacattaac ctgcggtttc agcctgtcgt
    31861 tcatgatttt aatggcgtcg cccaactgca tcgcgcgacc cgcaataaca acgcagggtt
    31921 gtgccagcag gcatagtgcg gcattttcgc cagattcaac gaccagcaaa ttgacctgct
    31981 cgtgagtatc gctcaaccaa acgcaggccg catcgcccgg catgggcacc cacatttcac
    32041 catgggaaac aaaatgccag cttttcggca tttgcggttt gagatagcga attgctacca
    32101 gtgcattcag caccagttcg gctttttgtt ctttagataa attaaaatcg cggcactttt
    32161 cttcaaaaga gaaatagagc gcggcgtcgt caacgcaaaa gcctgcggga gaaaaggcat
    32221 ctggagtcaa catcttgcgc gcaaaacgtg agcgaaatag cataccattg gctaaatcga
    32281 gcatcattcg atcgtgctct tcatcgtaat accaacgcca gttatcgtct ggtttaattc
    32341 gcatctgcct ctcccctacc ctataaaacc gcagcaacac aatccattgt cgcttcgctt
    32401 aataacctca acaataaaag tacaaaatgt ctaaataagc aacagcgatg aatataaaac
    32461 aaccagggct gaaaataaag ccctgattat cttataaaga ggaattcgaa gaaataatta
    32521 gatatgggta acgatttctt taatcagcgg cggaccttta aaaataaaac cagaataaat
    32581 ttgcaccagt gaggcacccg cagcaatctt ttcacgcgca gcgataaccg agtcgatgcc
    32641 gccaacaccg atgatcggta agcgaccgtt taattccagt gacaagcggc gaataatttc
    32701 ggtgcttttt aactgaagcg gacgaccact taagccaccg gtttgatcgc aatttttcat
    32761 tccctgaaca agagaacgat cgagtgtggt attggttgca ataacgccat caatattatg
    32821 gcgaactaaa ctatcggcaa cctggatcaa ttcttcttca gaaagatccg gcgcgatctt
    32881 cactgcgatc ggcacatatt tatggtgcat cgcttgcaaa tcattttgct tatttttaat
    32941 cgcggttaag agatcatcca gcgcttcacc atattgcagc gtgcgtaatc ctggggtatt
    33001 cggcgatgaa atattgatgg cgatatatcc cgcataggca tagatttttt ccatacaaat
    33061 cagatagtca tctttgccct gctccactgg cgtatcttta tttttgccga tgttaatacc
    33121 caggacgccg tcataatggg ccttttttac gttctctacg aggttatcaa cgccaagatt
    33181 attaaagccc atacggttga tcaaaccttc ggcatctacc agacgaaaga gacgcggctt
    33241 gtcattacct ggctgtggac gtggcgtgac ggtaccgatc tcgatcgatc caaatcccat
    33301 cgcgcctaac gcgtcaatgc actccccgtc tttatcaaga ccggctgcca gaccaagcgg
    33361 atttttaaac gtcaggccca tgcagttaac aggtttcgca ggcactttct gccgcaccag
    33421 tgcttcaaac ggcgttcctg taatacggcg taattgctga aaagtaaact catgagcgcg
    33481 ctctggatcg agctggaaaa gggctttacg aacgaagggg tagtacatga actctcctgg
    33541 attcccggtg tgcaaaccgg gggcgtatta tgtgcgagtt cggaccaaaa gggaattgac
    33601 ctgcggcaaa aaaaacgcaa acgttttccg aacctgtttc agggcagatt ttgctccttc
    33661 tttgcactca acccgccgta atcatttatc gcccgccagg cgacatgaca cggtgcgatt
    33721 gcctgacacc agcttgtctg acgctggctt ttaggcggca caactcccgc attacttaac
    33781 gctttaccat ttacgctcaa ctgaatcagg ttaatgtagt aaggcgttgg gttagtgagt
    33841 tgtacaccct gcgaattccg tgtccagcgt aattgttgat aagcttccag cgggtcgccc
    33901 ggcaaacctt cgggtcgcca gaacagttta aataccgagc gcatcgccac ttttacgctc
    33961 tgattttcaa ctttgccgga tggcacgctg gcaatgctta actcaaatag cgtttcgcga
    34021 tccacaggca agatgtcgct ttccgttctc agcaagcgca acgtgcctgt agtaccgggc
    34081 ttcaggagaa taagtggcgg agcggccagt aatggtgctg gcactgtcga cgcttccccc
    34141 ccggcccaac gcgttgggcg gttgatttta ctgttaataa gccaggattc ctgactggta
    34201 ttagtcagta aaatagatat cgattctctg tctgccggaa agataaatcg cgttccgccg
    34261 accaccactc ctgcccgcaa ttcacccgcc accagcagcg ataaaacggc gaaaatcaag
    34321 agcgccaatc tgttccacgt attagtcata atgaagtaca aatgtcgcgc tggcattcac
    34381 tttacccgac tgcacatcgc tattgacagg tcgcaattgc gcataaaaag ttaatggcac
    34441 cttaccgcta tgtaggctgt agtctttact cgcctggtta agcggaatca atatcttcgc
    34501 atcatccaga atcgcaattg ccagcccgct ggctgcatcc gtaccacttt caatggacag
    34561 catttcgctc tgcactccgt cgggggttcc tgaaaaggtg acgtcaaccg tgctgacatc
    34621 tgttccgcaa tcctgcaaat taatgatgaa agatttctcc gggccataat tcccatgaaa
    34681 aaaacgtgtc agcgagacat cgcctaactg cacattgatt tgcttgctat cctgtgccag
    34741 cacgcatgta ttacggcgaa acgatccgct aatgttgact gtgccatcat gggcaaatag
    34801 ctcacagccc cccgttaatc ccagtaagaa cagaatccag attatccgtt tcaacatcac
    34861 tccttcactg gtaaaccaga tcaaaataga gaaccgccgt cgcattaccg cccgttgctc
    34921 ccgcctttgt ggacttataa cgaagttgat atttgagtgt gttatccccg gcttttaagg
    34981 gcgtaagagg ctggacctga ttgagcggga tttcttgctg actttgcgca tcaagaattt
    35041 gcacggcaat ccccgttgcc ataccaccgc ttccgcctgt gtcagttagt ttgagcagtg
    35101 tcggcgcaag ggtgtcagcc tcgccgctaa aaaggacgtc cgcgccagta ataccagcgg
    35161 cacaaccggt gatatcgata ttcaaatcag cagcagtgct tacggtattc gcggcctgaa
    35221 aatcactgat attaaaatca ccaatatgaa tattttgtaa tgcacttttg gtggcgacat
    35281 cacagccctg atcgaccaca ttcccataaa tatgcatact aacttgttca ggcccagcca
    35341 tcccgacatg gatgttaaga atgctaaaga acaagataac gcactgaaat atcgtttttt
    35401 tcttcatacg ttcacgcgtt aatcaaaatt tattctcacc gttgccgtgg cttcaaatcg
    35461 cccggtttct ggtggtacac ctgtcgtgct gactggatag acattcattc gcgcgatact
    35521 gtctgcatca tcaggaatag agagcggtaa tgttccacca gcaggggaaa taatgttgtt
    35581 ctggctatcg gtcaccacca cgcccacgcc aggacgtgac gtcttaatcg cctggggata
    35641 gctggggtct gtggttgccg tcagactcag gttgacgtta actaaccccg cgtttcccgt
    35701 gttacacctg actggaattg cgagttctgc cgttctggcc ccaaggggtt tatgccctac
    35761 aacacgaaag ttagccgcaa atacgtcgcc cagatcgacc tcgatagtct ctccggcatt
    35821 gacggaacat gagccaagtg aattgatcgt accagacagg taatacacta aaaccgatgt
    35881 acctgtagtc gtgcaggaat cacctgcggg tatgttgtag cattcaaata atcgcgccag
    35941 cgccatagga ggaatgacga ccgaaccaac gaacggctgc aatattttca gggaaaagct
    36001 accgctatta ccgctagccc cctggccacc taaacgttga ccgccgggat cgcaaatatg
    36061 ccatcgtgta tctgcccctg tgcccacgtt ttcgaaagga accgcagtag gagaactgcc
    36121 tttaacctgc aataaaactt tggcggcaag atagtcatta attttgtacc agttgtcgcc
    36181 taaatagact aacggtgaat ccgcggcgaa tgcccatcgg ccacttctga cattgtcttt
    36241 atcgcaatca caggaaacat aataatcatt cgattcgttc cactgataaa atttgtcttt
    36301 aactactcca gccgtatttt cttcttcctc cagggtttta acgccaaaat tgacgccaat
    36361 atacggattc ccctgagtgg tatgacactg cccgctataa gcagcagttt gagcgcacca
    36421 tagcattaag acggcggcaa aaaatagtcg tagtaataac acgcattttt ctccaaagat
    36481 tatctgcata tggcatccat tcgcgtaatc gcagagttaa tttgttgttc ggtaagctgg
    36541 taactgatat gacaaatttg atcgggagct tcgccccagc gtacagttat cgctccctga
    36601 gcgggcaaac cggacatata gagcgagcca tcatcatcaa taatgccgct attagtctgt
    36661 atgccgtcca gcgtcgctat cgcgccaaaa cgcagcggta taccctgctt tgatgttttc
    36721 attaataccc ttgctcccgc atgggcattg aactccgcca gaaccaacgc ccctttggtg
    36781 ggaaccacgt tgactactgc attttcaaga tccacgttac gttttaacga tgccgcatca
    36841 agtgcgattc gattttcccg ataagaagtg gcatagggga caacggtata tccgcgccag
    36901 tcagttttca gcccggtgct gttcgccaca cgagtattct ctgcgcctgg tgctgcaatc
    36961 agcacgttag tttcccccag cgattgcccc agggtaatgc cctgtgaatg ggcaactaaa
    37021 ctgcctgaaa gagcgtagtt cagttgttgt tgactgccgt tatcgctgta gttgtagccc
    37081 actcgggcat ctgcaaacgc ccctttgtag tccatgctgg cgctaccatt agccgttttc
    37141 ccctcgctgt tatatccctg ttgcacgctg tagctcaggt taccgtcatc gagtagtgtc
    37201 tccgttaaac ccaggttttg tgtaatgtgc ccttcgttat cgatactggt gttagaggtc
    37261 atccaggcgt aatttttttt cctggtatag ctatcattac ttctggggag caacagattg
    37321 agcggcaggg aaaaatttag tgcaaacacc tgatcagcat caggttgacc acgggactta
    37381 ctgtagttcc aggaaacccc cagcgagaga tctttaattt gcgtgttgta gccaaactgt
    37441 aatagccgat cctgttgatc ggtatgccag taagtttgct ggctaccact taaataaaac
    37501 gaaccgtact cgcctaattg ctgggagata ttgacctgca gtttgccacg tttggtgtaa
    37561 aacaaattgt aataacgcga ccacatcggc gtatcttcat catcaccgtc attaaattcg
    37621 tagccatcca tatgtttata catggtgtcg gaaagggtgt agaaacccga cgtcgaatag
    37681 cgatagccca tcaattgaaa attagtgccc aattggttga gcgttttggc atacagaaaa
    37741 cgatatgact gacctttatg ttcgctttgg tctgccagcg tactcttcgc ctgggtcgca
    37801 tcgaacgaaa ttgctccgaa atcgccaagg ttaaatccca gaccaaacat ggcggcacgg
    37861 taatattcag catattgtcc accaccatac catgtcgttc cccacggtcc gccccactgc
    37921 aacgtggcct gggcaaattt gctctcctgc tgttcattac tattggttct gtatttcgcc
    37981 agtgtcaccg cgtatttgat tcgcccctga cgctggagta atggcacgct ggaaaacggt
    38041 acgctgtagc tattgacgct gccgtccgct tctttgattt caactaacaa atcaccgctc
    38101 gacgacgtgg aatagagatc actaatttca aaagcaccag gcgatacata agtttggtaa
    38161 atggtgtaac cattttgctt aatcgttatc tgggcattgc ttttggcgat accacgcacc
    38221 acaggcgcaa accctttcag gctgtctggc agcatgttgt catctgacgc cagctgcaca
    38281 ccacgaaagc tgacactatc gaaaaaatcg cctgcagtat aatcatcacc aacggtcagt
    38341 tcgcctttta gtggaataac cgcccgctgc aaatagctgc tgagattctt ccattctgcg
    38401 gtttggccac tactgcgact ccatgttgaa ttgttgcgca atctccatgg gcctaaatta
    38461 acgccactgt ttaaattcag aaaatagctg tcgccagaat cactgtctgc gctgctatga
    38521 atactgttag ccccgctaaa cgaatatccc agtaatagcg cgttgatccc ttcatcccaa
    38581 cgttcagggg ggacataacc acgggcatta cttttgatgg cgatttgcgg tactgaaatt
    38641 tccagacgga gtttattcac attaaacgtt acgctggcat ccggaataat ctccgccaat
    38701 ggcacacacg cttgtttgtc cagttgttta aaagccggaa acgcatcagt attaacaccc
    38761 attgcatcca ggctttcagt cgtcaaacag gcggcaatcc ccttctctgg cgttatctca
    38821 acaaaattga cgttgcgcgt atctacaatt gtctgattca gaacaatatc gacacgataa
    38881 acccctggcg ttattttttg tcctttttcg aaacgagaca aatcggccac cattgaggga
    38941 tcatctttga aaaaagcagg atcgaaccag gtttcagctt gtgcattgaa cgctgccgac
    39001 acaaacacca ccagcccacc aataaacgat ggcactccac cagagcttaa caggctgtgt
    39061 tgtcgatgag ttctatacat ctcgctctct taactgaaga cggcttacga tttacagttg
    39121 agttgcggcg tttccgcccc gtagtcattg atggttcgat acgttacgct gctatttttg
    39181 gccccctttg gcagagaaaa ttggtattta tcaaaggggg aaatcatttg atttttaact
    39241 acttttccac ctattttcag gtctgtcagg gtaatgaaat aaggtgttgg gttactgacc
    39301 gtaaccatgt cagctgacga agtgcaacgc agtttcgccg gagcctcgcc taagggatac
    39361 gctaaacctt ttggtcgcca gaaaagtttg atgacgcttt gtgtggcaat cttcagggta
    39421 tttccctctt cccgcttcgt tgttgaaggg actgcccgta cgctagtgaa gaaaagggtt
    39481 tctctgtctt tcgccaacgg cgcaccaatg tacataatgc gtaacagatt ctcattagcc
    39541 ggattcagca caaatagcgg tggtgtaata ataaaatctg ctgatttttt attatctggg
    39601 gtcatcaccc atgactgaat aagataagga acatccggac tggtgttacg tacacccagt
    39661 gaaatttgct cttcaccttc tgaataaaca agacgtgttg ctgcaagtcc aatcccgcct
    39721 ttacctgccg cccatgcgga tgagcagctg agtaatattg ccgtaaaact tacggtcaca
    39781 attccctttg ttatgcaagt tttcataaat agtcactctt aaaaaatatg aata
//
LOCUS       DAZFRN010000029        39675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFRN010000029 DAZFRN010000000
VERSION     DAZFRN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.029"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(182..877)
                     /gene="aqpZ"
                     /locus_tag="ACHKUB_001455"
     CDS             complement(182..877)
                     /gene="aqpZ"
                     /locus_tag="ACHKUB_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO6654493.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1303..2961
                     /gene="ybjD"
                     /locus_tag="ACHKUB_001456"
     CDS             1303..2961
                     /gene="ybjD"
                     /locus_tag="ACHKUB_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO6654494.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CADGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2958..3914)
                     /gene="ybjX"
                     /locus_tag="ACHKUB_001457"
     CDS             complement(2958..3914)
                     /gene="ybjX"
                     /locus_tag="ACHKUB_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO6654495.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4065..5180
                     /gene="macA"
                     /locus_tag="ACHKUB_001458"
     CDS             4065..5180
                     /gene="macA"
                     /locus_tag="ACHKUB_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO6654496.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5177..7123
                     /gene="macB"
                     /locus_tag="ACHKUB_001459"
     CDS             5177..7123
                     /gene="macB"
                     /locus_tag="ACHKUB_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO6654497.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7196..7420)
                     /gene="cspD"
                     /locus_tag="ACHKUB_001460"
     CDS             complement(7196..7420)
                     /gene="cspD"
                     /locus_tag="ACHKUB_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO6654498.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7570..7644
                     /gene="yljB"
                     /locus_tag="ACHKUB_001461"
     CDS             7570..7644
                     /gene="yljB"
                     /locus_tag="ACHKUB_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO6654499.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7743..8063
                     /gene="clpS"
                     /locus_tag="ACHKUB_001462"
     CDS             7743..8063
                     /gene="clpS"
                     /locus_tag="ACHKUB_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO6654500.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8094..10370
                     /gene="clpA"
                     /locus_tag="ACHKUB_001463"
     CDS             8094..10370
                     /gene="clpA"
                     /locus_tag="ACHKUB_001463"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO6654501.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10714..10801)
                     /locus_tag="ACHKUB_001464"
     tRNA            complement(10714..10801)
                     /locus_tag="ACHKUB_001464"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10765..10767),aa:Ser,seq:gga)
     gene            complement(11055..11273)
                     /gene="infA"
                     /locus_tag="ACHKUB_001465"
     CDS             complement(11055..11273)
                     /gene="infA"
                     /locus_tag="ACHKUB_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO6654502.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11558..12262)
                     /gene="aat"
                     /locus_tag="ACHKUB_001466"
     CDS             complement(11558..12262)
                     /gene="aat"
                     /locus_tag="ACHKUB_001466"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO6654503.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12304..14025)
                     /gene="cydC"
                     /locus_tag="ACHKUB_001467"
     CDS             complement(12304..14025)
                     /gene="cydC"
                     /locus_tag="ACHKUB_001467"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO6654504.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14026..15792)
                     /gene="cydD"
                     /locus_tag="ACHKUB_001468"
     CDS             complement(14026..15792)
                     /gene="cydD"
                     /locus_tag="ACHKUB_001468"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO6654505.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15915..16880)
                     /gene="trxB"
                     /locus_tag="ACHKUB_001469"
     CDS             complement(15915..16880)
                     /gene="trxB"
                     /locus_tag="ACHKUB_001469"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO6654506.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17425..17919
                     /gene="lrp"
                     /locus_tag="ACHKUB_001470"
     CDS             17425..17919
                     /gene="lrp"
                     /locus_tag="ACHKUB_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO6654507.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18054..22199
                     /gene="ftsK"
                     /locus_tag="ACHKUB_001471"
     CDS             18054..22199
                     /gene="ftsK"
                     /locus_tag="ACHKUB_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO6654508.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQQQYQQPQQPV
                     PPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQY
                     QQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALE
                     QMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTV
                     AVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVV
                     ADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIP
                     HLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPD
                     PYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHL
                     VLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPN
                     STLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPL
                     FDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPP
                     PFD"
     gene            22354..22965
                     /gene="lolA"
                     /locus_tag="ACHKUB_001472"
     CDS             22354..22965
                     /gene="lolA"
                     /locus_tag="ACHKUB_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO6654509.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22976..24319
                     /gene="rarA"
                     /locus_tag="ACHKUB_001473"
                     /gene_synonym="mgsA"
     CDS             22976..24319
                     /gene="rarA"
                     /locus_tag="ACHKUB_001473"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO6654510.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24410..25702
                     /gene="serS"
                     /locus_tag="ACHKUB_001474"
     CDS             24410..25702
                     /gene="serS"
                     /locus_tag="ACHKUB_001474"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO6654511.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25941..28385
                     /gene="dmsA"
                     /locus_tag="ACHKUB_001475"
     CDS             25941..28385
                     /gene="dmsA"
                     /locus_tag="ACHKUB_001475"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO6654512.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28396..29013
                     /gene="dmsB"
                     /locus_tag="ACHKUB_001476"
     CDS             28396..29013
                     /gene="dmsB"
                     /locus_tag="ACHKUB_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO6654513.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGALQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            29015..29878
                     /gene="dmsC"
                     /locus_tag="ACHKUB_001477"
     CDS             29015..29878
                     /gene="dmsC"
                     /locus_tag="ACHKUB_001477"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO6654514.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29914..30540)
                     /gene="ycaC"
                     /locus_tag="ACHKUB_001478"
     CDS             complement(29914..30540)
                     /gene="ycaC"
                     /locus_tag="ACHKUB_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO6654515.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30855..32003
                     /gene="ycaD"
                     /locus_tag="ACHKUB_001479"
     CDS             30855..32003
                     /gene="ycaD"
                     /locus_tag="ACHKUB_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6654516.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32213..33643
                     /gene="ycaM"
                     /locus_tag="ACHKUB_001480"
     CDS             32213..33643
                     /gene="ycaM"
                     /locus_tag="ACHKUB_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO6654517.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33644..34552)
                     /gene="ycaN"
                     /locus_tag="ACHKUB_001481"
     CDS             complement(33644..34552)
                     /gene="ycaN"
                     /locus_tag="ACHKUB_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6654518.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            34652..35242
                     /gene="ycaK"
                     /locus_tag="ACHKUB_001482"
     CDS             34652..35242
                     /gene="ycaK"
                     /locus_tag="ACHKUB_001482"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO6654519.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(35324..36064)
                     /gene="pflA"
                     /locus_tag="ACHKUB_001483"
     CDS             complement(35324..36064)
                     /gene="pflA"
                     /locus_tag="ACHKUB_001483"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO6654520.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(36256..38538)
                     /gene="pflB"
                     /locus_tag="ACHKUB_001484"
     CDS             complement(36256..38538)
                     /gene="pflB"
                     /locus_tag="ACHKUB_001484"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6654521.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(38593..39450)
                     /gene="focA"
                     /locus_tag="ACHKUB_001485"
     CDS             complement(38593..39450)
                     /gene="focA"
                     /locus_tag="ACHKUB_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO6654522.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9875 a         9685 c        10486 g         9629 t
ORIGIN      
        1 atccccttcg gctaccgggt cgggcgcgag gtaacgtcct gtaagacgcg ccctcagccc
       61 acatccatgt gggctgcccc ggccttcggg gaacgcttca gcgattttga cgccaccaac
      121 acccgtgcgg tttgtaacgt cgaagataaa acaaccaggg cagataaaat gccaatgcta
      181 attaattacg tttttccagc agggtccggt aaatcagacc accgataatg ccgccgacaa
      241 ttggcaccac ccagaagaac cacagttgtt ctaatgccca gccgccctgg aagatagcaa
      301 ccgcggtgct gcgcgccggg ttaacagaag tgttagtcac cggaatacta attaagtgaa
      361 tcagggttaa ggccagacca atagcgatcg gcgcaaaacc tgccggcgcg aatttgtcgg
      421 ttgcgccgtg gatcaccaac aggaaacctg cactcaatac cagttcaact accagcgcgg
      481 aaagcatgga ataaccgcct ggtgaatgct cgccataacc gttagaagca aaaccgctgg
      541 ctgccgcgtc aaaacccgtt ttaccactgg caattaaata cagcagcgcc gctgcaacaa
      601 taccgccgac aacctgggca attacgtagc caacgacttc ttttgccgga aaacgtccgc
      661 cagcccataa accaatagtg accgccgggt taaaatgacc accagaaata tgaccaacag
      721 caaaggccat cgtcagaacg gtcagaccga acgccaacgc cacgccggca aaaccaatgc
      781 ctaattccgg gaagcctgcg gccagtacag cactaccaca gccaccaaaa acaagccaga
      841 aagtaccaaa acattcagct gctaattttc tgaacatatc cacctcaatt aaaaattgac
      901 cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa aagtgacgac ggaaataatg
      961 cgcggctatt ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg
     1021 atttagggca acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa
     1081 gcaaaagatt taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt
     1141 ttgttgtttt tgcagactta tcagcaagag ggagtataac gcgattattc gctcattttt
     1201 cagacatttg ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat
     1261 actgccagcg taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt
     1321 gaaattgtgg gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc
     1381 ctgattgggg agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta
     1441 tcgccagaat cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat
     1501 atcaacgggc gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc
     1561 cgacatcggg ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg cgccgatggc
     1621 tatcaccgta ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca
     1681 ctgcgcagtt ttctcgataa agacggacat ccgattgatg tcgaggatat taacgatcag
     1741 gcacgccatc tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc
     1801 cgtattcgta acggcacggt gccaaatgtt cccaatgtgg aagtcaccgc gcgccagctc
     1861 gatttcctcg cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt
     1921 cagggacttt ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggccgga
     1981 caggcgcgat atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat
     2041 ctggacatca tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg
     2101 ctcggcctgt ttgctacttt attgcaggca aaaggcacat tgagactgga taaagacgcg
     2161 cgtccattgt tgctgatcga agatccagaa acccgtttac accccattat gctttcagtt
     2221 gcctggcatc tgttgaatct tctgccattg cagcgcattg ccaccaccaa ctcgggtgag
     2281 ttgctttcgt taacgccggt agagcatgtt tgccgactgg tacgtgagtc ctcgcgcgtt
     2341 gccgcctggc gtctggggcc gagtggcttg agtaccgaag atagccgacg catatccttt
     2401 cacattcgtt ttaaccgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg
     2461 gaaacggaaa cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc
     2521 gaagggatca aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc
     2581 cgccgaatgg ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat
     2641 gccgctacgg tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg
     2701 gcgttaccgg cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc
     2761 caccgcgtgg cgcaaatccc ggaaaatgta ccgatgaatc tgcgcaaaat tatctcgaaa
     2821 gcgatccatc gctcttccaa acccgatctt gccattgaag tggcaatgga ggccggacgt
     2881 cgtggtgtgg actccgtacc gacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg
     2941 gcgcgcggtc gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg
     3001 agcatctcat agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct
     3061 tttcgtgcta tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc
     3121 gattcccaga aagcgttgta atcggcatgg atcttgcctt ctttatcacg ataacgcagg
     3181 ctgcggtaaa tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg
     3241 gcaaacagac aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc
     3301 gtcgcattct ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata
     3361 aacatcgttc ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata
     3421 ccttcgctgt tgcgaaacag gattgtgctg tcaccttctt tatccattga gatcatcatg
     3481 gtcagctcaa gcgtgaactg ctcgccgttt ttgccttcca gcttcgccag ttgcagcccg
     3541 ggggtattca aatataagct gaattcttcc gccgacatac atccgcggag taacgcataa
     3601 tggtaacgta acgcctccag caattgctta cggctaagat tcgccgcaag gtaagggcga
     3661 tgcagacgca caggcagtcg cggttggcgc gttaacaaca cattgagatt aggccagtgg
     3721 gaaagttcgt tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat
     3781 ttctggcgaa aactacggcg atgccagaat ttacccggcc gacactgtcc acgtgccaga
     3841 ctaagaaaaa gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc
     3901 tgcgacatat tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt
     3961 aaccaaacat caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt
     4021 gaagttaatg acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa
     4081 ccgtgaagaa gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat
     4141 ggagaattct taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt
     4201 tacagcaaag cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg
     4261 cgcaggtcag cggtcagttg aaaactctgt cggtggcgat tggcgataaa gtaaaaaaag
     4321 accagctttt aggggttatt gatcctgaac aggctgaaaa ccagattaag gaggtcgaag
     4381 caacgctgat ggagctacgt gcgcagcggc agcaggcgga agcggagctg aaactggcgc
     4441 gggtgacgta ttcccgtcag caacgtctgg cacaaacgca ggcggtttca cagcaggatc
     4501 tcgacaccgc cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc
     4561 aaatcaagcg caatcaggcc tctctcgata cggctaaaac caatctcgat tacactcgca
     4621 tcgttgcccc gatggccggg gaagtcacgc aaatcaccac tctgcaaggc cagacggtga
     4681 ttgccgcaca acaagcaccg aacattctga cgctggcaga tatgagcacc atgctggtaa
     4741 aagcgcaggt ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta
     4801 cggtacttgg cgatccactg acgcgctacg aggggcaaat caaggatgta ctaccgacgc
     4861 cggaaaaggt taacgacgct attttctatt acgcccgttt tgaagtcccc aaccccaatg
     4921 gtttgctgcg gctggatatg actgcgcaag tgcatattca gctcaccgat gtgaaaaatg
     4981 tgctgacgat ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca
     5041 aattgttgcg taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata
     5101 ccgatgttga gattgtcaaa gggcttgaag cgggcgatga agtggtgatt ggtgaggcca
     5161 aaccaggagc tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc
     5221 tgccggtgat gagcaggttg aggtgctgaa gggcatcacc ctcgatattt atgccggtga
     5281 gatggtagcg attgttggcg cttcaggttc cggtaaatcg accctgatga atattctcgg
     5341 ctgtctggat aaggccacca gcggcaccta tcgcgtcgcc ggtcaggatg ttgccacact
     5401 ggacgccgat gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta
     5461 ccatttactt tcgcatttaa ccgccgagca gaacgttgaa gtacccgccg tctatgccgg
     5521 tcttgagcgg aaacagcggc tgcttcgcgc ccaggggtta ctgcaacgac tggggctgga
     5581 agaccgtaca gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat
     5641 cgcgcgggca ttgatgaacg gtggtcaggt aattcttgcc gatgaaccaa ccggcgcact
     5701 ggacagccat tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgtgggca
     5761 tacggtgatt atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga
     5821 aattcgcgac ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg
     5881 cgggacggag cctgttgtca acacggtgtc tggctggcgg cagtttgtca gcggttttaa
     5941 cgaggcgctg acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac
     6001 catgctgggg attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc
     6061 cgccaaacaa atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta
     6121 tcccgggaaa gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga
     6181 cttaatcgcc atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa
     6241 cctgcgcctg cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga
     6301 ttattttaat gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct
     6361 gaacggtcgt gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca
     6421 taaagcagat gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg
     6481 tgtggcggaa gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc
     6541 ttacagcacg atgtccgggc gagttatggg ccagtcgtgg ctgaactcca ttactgtcag
     6601 ggtaaaagaa ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact
     6661 gcgccacgga aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga
     6721 aaagaccaca cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt
     6781 gggcggtatt ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat
     6841 tggcattcgc atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga
     6901 agccgtactg gtttgcctgg tcggtggcgc gttgggaata actctgtcac tgttaattgc
     6961 tttcaccttg cagcttttct tacccggctg ggagattggt ttttcaccgt tggcactgct
     7021 gctggcgttt ctctgctcga cggccaccgg gattttattt ggatggttac ccgcacgaaa
     7081 tgcggcacga ctggatccag tagatgctct ggcacgagag taatttttga gataaaaatg
     7141 ccagccgatc gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg
     7201 cgactgccgc ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct
     7261 ggtggacatc aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa
     7321 tggtggaata atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc
     7381 ctttggcatt gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa
     7441 tcttatacaa gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc
     7501 aactctagaa atacaattta gagaattagg gcgagccgtc aagcgtttga caggggccag
     7561 ggggcaggta tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat
     7621 gtcgctgatg ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa
     7681 ataagctctt gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga
     7741 caatgggtaa aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg
     7801 acgcgctaaa accgccatct atgtataaag tgatattagt caatgatgat tacactccga
     7861 tggagtttgt tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc
     7921 aattgatgct cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg
     7981 ttgcagaaac caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc
     8041 tgtgtacgct agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca
     8101 atcaagaact ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg
     8161 agtttatgac cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg
     8221 cgctggaagc gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg
     8281 aacaaaccac acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga
     8341 gttttcagcg tgtactgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg
     8401 taaccggtgc aaacgttctg gtcgctatct ttagcgaaca ggagtcgcag gcggcatatc
     8461 tgttgcgtaa acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc
     8521 gtaaagacga gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag
     8581 ctggtgggga ggaacgtatg gagaatttca cgacgaacct gaatcagctt gcgcgcgtgg
     8641 gcggaatcga cccactgatt ggtcgtgaga aggagctgga gcgtgctatt caggttctct
     8701 gccgtcgccg taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga
     8761 ttgcggaagg tcttgcctgg cgaattgttc agggcgatgt gccggaagtg atggctgact
     8821 gtacgattta ctctctcgat atcggttctc tgttagcggg cactaaatat cgcggcgact
     8881 ttgaaaaacg ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt
     8941 ttattgatga gatccacacc attatcggtg cgggggcagc gtctggtggc caggtcgatg
     9001 cggctaacct gatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa
     9061 cctatcagga gttcagcaac attttcgaga aagaccgtgc tctggcgcgt cgcttccaga
     9121 aaattgatat tactgaaccg tcgatcgaag aaactgttca aatcatcaat ggcctgaaac
     9181 cgaagtatga agcgcaccac gacgtgcgtt ataccgcaaa agcggtgcgt gcagcggtag
     9241 agctggcggt gaaatacatt aacgatcgtc atctgccgga taaagccatt gacgttatcg
     9301 acgaagcggg cgctcgcgca cgcctgatgc cggtaagcaa acgcaagaaa accgttaatg
     9361 tggcggatat tgagtccgtg gtggcccgta ttgcgcgcat tccagagaag agtgtttctc
     9421 agagtgaccg cgataccctg aaaaacctcg gcgatcgcct gaaaatgctg gtcttcggtc
     9481 aggataaagc cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc
     9541 acgaacataa accggttggt tcgttcctgt ttgccggccc taccggggtc gggaaaacag
     9601 aggtgacggt acagctttcg aaagcgttgg gcattgagct gctgcgcttt gatatgtccg
     9661 agtatatgga acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt
     9721 ttgatcaggg aggtttgctg actgatgcgg tcatcaagca tccacatgcg gtgctgttgc
     9781 tggacgaaat cgagaaagcg catccggacg tgttcaatat tctgttgcag gtgatggaca
     9841 acggtacgct gaccgataac aacggacgca aagcggactt ccgtaacgtg gtgctggtga
     9901 tgaccaccaa cgccggggta cgtgaaactg agcgtaaatc cattggtctt atccaccagg
     9961 ataacagcac cgatgcgatg gaggagatca agaagatctt tacaccggaa ttccgtaacc
    10021 gtctcgacaa cattatctgg tttgatcatc tgtcaaccga cgtgatccat caggtggtgg
    10081 ataaattcat cgtcgagttg caggttcagc tggatcagaa aggtgtttct ctggaagtga
    10141 gccaggaagc gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc
    10201 cgatggcgcg tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg
    10261 gttcgctggt ggacggcggt caggtcaccg tcgcgctgga taaagagaaa aatgagctga
    10321 cttacggatt ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtt
    10381 aggtaggttg gtcaagtccg taatctcgaa agaggttacg gactttttgt ttatggggtg
    10441 gaggaggttc agaccctttt tttaatgatg atggtaagtt gttgataatt agtgctgcgg
    10501 gaaggtaagg ataaaaaagg gtgctgcagg agaatgggat ggttttgctt tattaacaac
    10561 gggctaaacg tgtagtattt gagttcactg ccgtacaggc agcttagaaa tcgacggggt
    10621 gcggtaaaac ctttgcgaac gcgttcactg ccgtacagac agataaaatg cgaaaaaaaa
    10681 gctcgcactt tcgtacgagc tcttctttaa atatggcggt gaggggggga ttcgaacccc
    10741 cgatacgttg ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac
    10801 caaattgttt tgtcgcctga cctcatgggt ggcaacgggg cgctactata gggagttgga
    10861 gtaaaacggt caagaagaat tttaatgata attattgttt gctcatactg taaacaagtt
    10921 gtgcagtata tctacatcga gacaagttac ggacttatac ttccaaagta cttcatacat
    10981 atcacaaaat aaaaaggccg gttaaaccga ccttttactc gttctttctc ttcgcccatc
    11041 aggcggtaaa acaatcagcg actacggaag acaatgcggc ctttgctcag gtcgtacggg
    11101 gtcagttcaa cagtcacttt gtcgcccgtc aggatgcgga tgtagttttt gcgcatttta
    11161 ccggagatgt gtgcagtaac cacgtgaccg ttttctaact ctacgcggaa catggtatta
    11221 ggcaacgttt caagaacggt accttgcatt tcaatattgt cttctttggc catctaatcc
    11281 tctggggtat cactaccgta atttgaaccg gcaagataat gccgaagttc tgtaaataag
    11341 taaagatttg cgcgctaaat cgcaacaaac aggttcggca cattactccg aaaacacacg
    11401 gctaagccgc accaaaagcg caacgtataa gggagcggtg agataaacga tgggcgttac
    11461 ctgacgcgaa aaattcctta tcggcagcgg ggtaatgagc gtaaccaact ctgcgaccgc
    11521 aattataaca ctctggggag aaatgtgccg aaaacattca ttcttgtggt gaaaacaagc
    11581 atcgtggtac ccagaaatta ttcggcaatc gtccgaggcg catttgattg agataattaa
    11641 ggtaatcccg gcggggaatt tcgcaggcac caagcgatgc tgtgtgatcg ttaaggacct
    11701 ggcagtcgat aagcttaccg ccatgaccga taaattcctc acagaatacc agaagcgccg
    11761 ttttagacgc attttccatc cggctgaaca tggactcgcc acaaaatagc gttccctggg
    11821 ccacgccgta cataccgccg acaagctcat cttcacgcca gacttcaatg gagtgggcat
    11881 ggccgagttc gtgaaggcga tggtaggctt cgaccacgcc acgcgtgatc caggttcctt
    11941 cttcgcgatc gctggcacag ccttcaatga cctgaccaaa agcgtaattc atcgtgacac
    12001 gatagggcga gcgtttatga aatcgcttca tactacggct gatatgcagt gattctggcc
    12061 atagcaccgc gcggggatcg ggcgaccacc agaggatggg gtcgcctgga gaaaaccacg
    12121 gaaaaatacc acgctggtaa gccattaaca ggcgcgcagg gctaagatcg cccccaagtg
    12181 ccagcaggcc gttaggctca cgtaatgcgc cttccgggga agggaaggct attgaatggc
    12241 gagaaagctg aaccaggcgc atgaccgcaa aactccacgc aagtcggatc gttcaataat
    12301 agcttacaaa ccctgcttga actggtaata acgcccctgt ctggcaagca gctctgcgtg
    12361 agtaccttgc tcaataattt gcccgttgtc catcactatt atttgttgga aacgagagag
    12421 tccgcgaagt cgatgggtga ccattaacac cgttttctca cgcatcattt ctgcaagcaa
    12481 ttcaaggatc tggctttcgg ttgtggcatc taagccttcg gtaggttcat ccagcaacac
    12541 cagtggcgca tcatgtaaca gcgcacgggc gatagccaga cggcgcagtt caccaccgga
    12601 gagctggcgt ccgccttcac ctaaccaact gttgagacct gcatcctcga gcagcttctc
    12661 caggccaacg cgacgcaagc tctccgccag agcctcatca ctactgccag gcgaggcgag
    12721 taacagatta tcacgcagcg tggcgctaaa cagatgcact cgctgaggaa caacgctgat
    12781 ggtctgtcgt agagccgctt cattcaggct ggctatgggg ctatcgttaa gcaaaatctc
    12841 gccctgttgc gggtcccaag cgcgggtcag cagttgtaac agtgttgatt tgccgcatcc
    12901 ggttcgcccg agaatcgcaa tatgttcccc ggcgtttacc tgaagagaaa tccctttaag
    12961 tgcctgttgg gattgctccg gataagtgaa ctgaacatcc cgtaatgtca gcgaaacgcg
    13021 atcggcaaca cgagtttggg tatcagggaa ggtgacttcc ggtttttgat ccgttaaatc
    13081 agtgatccga acggcagagg caatgacttg ccccagatgc tgaaatgcac ccgttactgg
    13141 tgccagtgct tcaaacgcgg ctaacgcgca gaagacaaac agggcaatta acgcgccggg
    13201 ttgagcattg ccgccaacgc cgccagacgc catccacagc atcaggatca ccgctaacgc
    13261 gccaatgagt agcattatcg cttgcgacaa tgcggtcagt tcagattgac ggcgttgcgc
    13321 ttccagccat tgaatttctg tattctctag ttgcgtgcga taacgatcgc tggcaccaaa
    13381 aatggtcagc tcagcttgcc cttgcagcca ggccgtcagt tgttggcgat actgtccgcg
    13441 aagatgagtc agattttgcc cggtgctttt tcccgcacga taaaacagcg gtggcatcag
    13501 gaaaagcgtc agtaacataa tgccgcccag cgtaaaggcg agggtgaaat caaggaaact
    13561 taacccgatt gtcaccacca taatcaccac aaaagcgccc accagcggcg agataacgcg
    13621 cagataaaga tgatcgagcg tatcaacatc cgccaccacg cgattgagca attcgccctg
    13681 acgatagcgc gccagtccgg caggggagag gggcagcaat ttgctgaagg tgtaaatgcg
    13741 cagatgctgc aacacgcgga aagtcgcgtc gtgacttacc agacgttcaa aataacgccc
    13801 ggcagtacgg gtgattgctg cgccacgcac gcccgcagcg ggtagcatat agttgaagct
    13861 gtacagtccg gcaacccccg caaccgctga ggccgagagg aaccagccgg aaagtgtcaa
    13921 cagaccgata ctggcgagca gcgtgacaat tgccagcacg ataccaagac ttaacatcca
    13981 tttatgacgt ttatacagtg ccagataggg tagcaaagcg cgcatttaaa tctcctcctg
    14041 acgatgggcc agtaatgtgg cgaatgggcc accagccaca cttaattccg cgtaacgtcc
    14101 ttgctcaata atccggccat cctgcataac ccaaatgaca tcccagtcag caagatcttc
    14161 taactggtgg gtgaccatta acgttgtctg gcgcagagag gcggcattca gcgcctccat
    14221 tacgcgctgt tcactgtgag catcaaggct ggcagcgggt tcatccaaca gtaatagcga
    14281 acagggattt agtaacgcac gggccaccgc cacgcgctgc gcctgcccca cggaaaggcg
    14341 ggcagcctgg tcgccaacag gcgtatcaac gccttgtggc aggagcggta gaaactcgct
    14401 gacccaggcg ttatccagcg ctgcttgtaa ttcttgttcg ctggcatcag gtcgcgccag
    14461 tagtacgtta tcccgcaatg ttgctgccgg taattgtggg ttttgcccaa cccaggagag
    14521 atgtttacgc catgattctg gtgataaatc gcgtaattct atcccgttga ttcgtaatga
    14581 tccctgatat gaaagaaaac cagaaagcgc gttcagcagt gagcttttac ctgaaccgct
    14641 gcgaccaacc aacactgcac gttggcctgc tggcaaagta aagttcagcg gtccggccag
    14701 cgttttacct tccggcgacg tgataaacag atcctcggcc tcaatggtca ccggatcggt
    14761 cgatgctaat tccgcctcac cgcgctgcgg atgggcgagc ggggtttcca taaacgtttt
    14821 cagactgtca gctgcaccaa cagcctgggc tttagcatga taaaacgtac cgagatcgcg
    14881 caatggctgg aaaaactctg gcgcaaggat cagggccaga aaacccgcag ccagcgtcac
    14941 accggtatcg tagtgaccaa aatccagctc gccgagatag gaaaaaccaa agtagaccgc
    15001 caccagagca attgacagcg aggtaaaaaa ttcgagaatg ccggaggata aaaacgccag
    15061 ccgtagcact tccattgtcc gttggcggaa atcttccgaa gcagaacgaa tactttcaat
    15121 ttcagcttca ccacgaccaa aaatacgcaa tgtttccatg ccgcgcaggc gatcgaggaa
    15181 atgcccactt aagcgagcaa gagcgagaaa gttacgccgg ttagcatcgg cagcccccat
    15241 cccaaccagc gccataaaca acggaattaa cggtgctgtg cccagcagaa tgagcgccgc
    15301 agcccagtta gaggggaaga tcgccaccac aatcagcaac ggcaccgaca ctgccagcgc
    15361 catttgcggc aggtagcgtg catagtaatc atgcatatcg tcaatttgct cgagtaccaa
    15421 cgtcgcccag ctccccgcag gtttaccctg aatccacgct ggccctgctt gttgcagacg
    15481 gtcgagaacc tgacggcgga tggcaaagcg gatatgctgc ccggcgtgat aacccacccg
    15541 ttcgcgtaac cagaccaccc atgcgcgcag tacaaaggtc agaaccagta acgtaaaggg
    15601 aagcagcagg gcttcacggg gaatattctc cataatcata tgttgcagaa ttcgcgccat
    15661 gaaccaggcc tgggcaatga tcaatatgcc gcttacaaag cccagcagac gagaaatatt
    15721 cagccaacgt tgggagatga cgctttgctg ttttaaccag cgggttaact ctttttgacg
    15781 ggatttattc attgcacgct tagcaggtga gttatcagaa ttatttgcag agcaatgtta
    15841 caacggggaa aaaataaagg cgacccatag tcgcatggtg tcgccttctt tacttttgtt
    15901 actgatttgt aaaattattt tgcgtcagct aaaccatcga ggtagcgttc cgcatcaagt
    15961 gctgccatgc agcctgtacc ggccgaagta atggcctgac gataaatatg atccatcacg
    16021 tcacccgcgg caaagacgcc agggatgctg gtctgggtgg cattaccatg aatacccgac
    16081 tgcactttga tgtagccgtt ttccagttcc agctgccctt cgaaaatggc agtattcggg
    16141 ctgtgaccga tagcaacaaa cagaccagca acgtcgagtg actcgatgtt atcgctgttt
    16201 tgcgtatcgc gcagacgaac gccagtgaca cccatttgat cgccggtcac ttcttccagc
    16261 gtacggttgg tgtgcagaat gatgttgccg ttctccactt tatccatcag acgcttaata
    16321 aggatttttt ccgcgcggaa accgtcacgg cggtgaatca gatgcacttc cgaagcgatg
    16381 ttagacagat acagcgcctc ttcaaccgcg gtattgccgc cgccgatgac cgcaactttc
    16441 tggttgcgat agaagaaacc gtcgcaggtt gcacaagcag aaaccccacg gcctttaaag
    16501 gcttcttcag agggcaggcc gagatagcgt gcagaagctc cggtggcaat aatcagcgcg
    16561 tcgcaagtgt attcgccgtt atcgccattc agacggaacg gacggttttg cagatccacc
    16621 ttgttgatat gatcaaaaat gatctcagtt tcaaacttgg tggcatgttc gtgcatgcgc
    16681 tccattaata acggaccggt cagatcgttt ggatcgccag gccagttttc cacttccgtg
    16741 gtggtggtca gttggccgcc tttttccatg ccggtaatca gcacaggttg caggttggcg
    16801 cgcgccgcgt agacagcagc ggtgtatccc gccgggcctg aacccaggat aagcagttta
    16861 ctgtgtttgg tcgtgcccat gagatcccca tagttgttgg cagacaatgg gcaggattgt
    16921 agggaattta cagacgtaaa aaaagagtat gacgattttg ttaacaattt gtgcaatcgg
    16981 cagcatcgat aagcaggtca aattctcccg tcattatcac ctctgctact taaatttccc
    17041 gctttataag ccgattaaat gatgaataaa cgcccctgtt aatgaatatc tggcatgttg
    17101 tactaaaaat cgatgttttg ctttgacaat cccctggtgt tttgcgaaaa cattcgagga
    17161 agaaaaaaaa cagtattctt atatgcgcat aaccatgcat gtaaatacca tgtttaccgt
    17221 gctagtgaaa tctacgtatg gcgtggacag acgccattcg tgatgtcgat agctgccaca
    17281 aggcaacggt cttctcaccg tagacccagg cattgcgcgc cgtgaatctt catgatttcg
    17341 gtctatcgtg acgggtagcg actctgaaca gtgatgtttc agggtcagac aggagtaggg
    17401 aaggaataca gagagacaat aataatggta gatagcaaga agcgccctgg caaagatctc
    17461 gaccgtatcg atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat ttctaacgtc
    17521 gagctttcta aacgtgtggg actttcccca acgccgtgcc ttgagcgtgt gcgtcggctg
    17581 gaaagacaag ggtttattca gggctatacg gcgctgctta acccccatta tctggatgca
    17641 tcacttctgg tattcgttga gattactctg aatcgtggcg caccggatgt gtttgaacaa
    17701 ttcaataccg ctgtacaaaa acttgaagaa attcaggagt gtcatttagt atctggtgat
    17761 ttcgactacc tgttgaaaac acgcgtgccg gatatgtcag cttaccgtaa gttgctgggg
    17821 gaaaccctgc tgcgtctgcc tggcgtcaat gacacacgga catacgttgt tatggaagaa
    17881 gtcaagcaga gtaatcgtct ggttattaag acgcgctaac acggaacagg tgcaaaatcg
    17941 gcgtattttg attacactcc tgttaatcca tacagcaaca gtactggggt aacctggtac
    18001 tgttgtccgt tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct ttcttgagcc
    18061 aggaatacac tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc cgtcgccttc
    18121 tggaagcgtt gctgatcctt attgtcctgt ttgccgtctg gttgatggct gccttactaa
    18181 gctttaaccc ttcggacccc agctggtcgc aaacggcctg gcatgaacct atccataatt
    18241 taggtgggat gcccggtgcg tggctggcag atacgctgtt ctttattttt ggcgtgatgg
    18301 cttacaccat tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt catcagtcca
    18361 gtgacgaata cattgattat tttgccgttt cgctacgcat cattggcgtt ttggcgctca
    18421 tccttacctc ctgtggtctg gcggcaatca acgctgacga tatctggtat tttgcctccg
    18481 gtggcgtcat tggcagctta ctaagcacta cgctacaacc actgctacac agtagcgggg
    18541 gaactattgc gctgctctgc gtttgggctg cgggcctgac gctgttcacc ggttggtcat
    18601 gggtgaccat tgctgaaaaa ctcggcggct ggattttaaa cattctcacc ttcgccagta
    18661 atcgtacccg tcgcgatgat acctgggtcg atgaagatga atatgaagat gacgaagagt
    18721 atgaagatga aaaccacggc aaacagcatg aatcacgccg tgcccgtatt cttcgcggcg
    18781 cgctagcgcg tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg cggcaaacag
    18841 acgctgcgtt gttctccggt aagcggatgg atgatgacga agagattatc tacactgcac
    18901 gcggtgtggc tgccgacccg gacgacgtcc tattttcggg caatcgtgca acgcagccag
    18961 aatatgacga atacgatcca ttattaaacg gtgcgccaat taccgaacct gtcgctgtag
    19021 cagctgctgc taccacggcg acacaaagct gggctgcgcc ggttgaacct gtgactcaga
    19081 cgccgcctgt tgcctctgtt gatgttccac cttcgcaacc tacagtagcc tggcagcctg
    19141 taccgggtcc acaaacggga gagccggtta ttgctcctgc accggaaggt tacccacagc
    19201 agtcacaata tgcacagcct gcagtgcaat ataatgagcc gctgcaacaa ccagtacagc
    19261 cgcagcagcc gtattatgca cctgcagctg aacaacctgc gcaacaacct tactacgctc
    19321 ctgcggcgga acaacctgtt cagcaaccgt attacgcccc tgcgccagaa caaccggtgg
    19381 caggtaacgc ctggcaagcc gaagagcagc aatccacttt tgctccacag tctacatacc
    19441 agactgagca aacttatcag caaccagccg ctcaggagcc gttgtaccaa cagccgcaat
    19501 ccgttgaaca gcagcctgtt gtggagcctg aacccgttgt agaagagaca aaacccgcgc
    19561 gtccgccgct ttactatttt gaagaagtgg aagagaagcg agcccgtgaa cgtgaacaac
    19621 ttgcggcctg gtatcaaccg attccagaac cggttaaaga accagaaccg atcaaatctt
    19681 cgttgaaagc gccttctgtt gcagcagtac ctccagtaga agccgctgcc gctgtttctc
    19741 cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg ggctgccgca accgttgccg
    19801 cgccagtctt cagtctggca aatagcggtg gaccgcgtcc gcaagtcaaa gaggggattg
    19861 gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac tcgtcgtgaa ctggcgtctt
    19921 acggtattaa gctgccctca cagcgtgcgg cggaagagaa agcccgtgaa gcccagcgca
    19981 atcagtatga ttctggcgat cagtataacg atgatgaaat cgatgcgatg cagcaggatg
    20041 aactggcacg tcagttcgcc cagacacagc agcaacgcta tggcgaacag tatcaacatg
    20101 atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga ggctgaactg gctcgtcagt
    20161 ttgcgcaaac tcaacaacaa cgttattccg gcgaacaacc ggctggggcg aatccgttct
    20221 cgctggatga ttttgaattt tcgccaatga aagcgttgct ggatgatggt ccacacgaac
    20281 cgttgtttac gccaattgtt gaacctgtac agcagccgca acaaccggtt gcaccgcagc
    20341 agcagtatca gcagccgcaa caaccagttc cgccacagca gcaatatcag cagccgcaac
    20401 aaccggttgc accgcagcag cagtatcagc agccgcaaca accagttccg ccacagcagc
    20461 aatatcagca gccgcaacag ccggttgcgc cgcagccgca gtatcagcag ccacaacagc
    20521 aggttgcgcc gcagccacaa tatcagcagc cgcaacaacc ggttgcgcca cagccgcaat
    20581 atcagcagcc gcaacaacca gttgcgccgc agccgcagta tcaacagccg caacaaccgg
    20641 ttgcaccgca gcagcaggat accctgcttc atccgctgtt gatgcgtaat ggcgacagcc
    20701 gtccgttgca taaaccgacg acgccgctgc cttctctgga tttgctgaca ccgccgccga
    20761 gcgaagtgga gccggtagat acctttgcgc ttgaacaaat ggctcgcctg gtggaagcgc
    20821 gtctggctga tttccgtatt aaagccgatg tcgtcaatta ctctccgggg ccggttatca
    20881 ctcgctttga acttaacctg gctcctggcg taaaagcggc gcgcatttct aacttgtcac
    20941 gggaccttgc ccgttcactt tcgacggtgg cggtgcgtgt cgttgaagtt attcctggca
    21001 aaccctatgt aggtctggag ttaccaaata aaaaacgaca aaccgtttat ctgcgcgaag
    21061 ttttggataa cgccaaattc cgcgataatc cgtcgccatt aaccgtggtg ctgggtaaag
    21121 atatcgccgg tgagccggtg gttgccgatc tggcgaaaat gccgcacttg ttggttgcgg
    21181 ggactaccgg ttccggtaaa tctgtcggtg tgaacgcgat gatcctgagc atgctttata
    21241 aagcacagcc agaagatgtg cgtttcatca tgatcgaccc gaaaatgctg gagctttcgg
    21301 tttatgaagg cattccgcat ctgttaacgg aagtcgttac tgatatgaaa gatgccgcca
    21361 acgcgctgcg ctggtgtgtt aacgagatgg agcgtcggta taaactgatg tctgcgctgg
    21421 gtgtgcgtaa tctggcgggt tataacgaaa aaattgctga agccgatcgc atgatgcgtc
    21481 cgattccaga cccgtactgg aagccgggtg acagtatgga tgcccagcat ccggtgctga
    21541 aaaaagaacc atacattgtg gtgttggttg acgaatttgc cgacctgatg atgacggtag
    21601 gtaaaaaagt ggaagagctg atagcacgtc tggcgcaaaa agcccgtgcc gcgggtatcc
    21661 acctcgtact ggcaactcag cgtccatcgg ttgatgttat tactggtctg attaaagcga
    21721 atattccgac ccgtatcgcc tttaccgtat ccagtaagat tgactcacgt accattcttg
    21781 atcaggctgg cgcggaatca ctgctgggga tgggggatat gctctactct gggccgaact
    21841 ccacgttgcc ggtacgtgtc catggtgctt ttgttcgcga tcaggaagtt catgccgtgg
    21901 tgcaggactg gaaagcgcgt ggtcgcccac agtatgttga tggcatcact tccgacagtg
    21961 aaagcgaagg tggcgcgggc ggtttcgatg gcgctgaaga actggatccg ttgttcgatc
    22021 aggcggtgca gtttgtcact gaaaaacgca aagcgtcaat ttctggcgta cagcgtcagt
    22081 tccgcattgg ttataaccgt gcagcgcgta ttatcgaaca gatggaagcg caggggattg
    22141 tcagcgaaca ggggcacaac ggtaatcgtg aagtgctggc cccaccgccg tttgactaat
    22201 taatgcattg ccggataagg cgcggtagcg tcgcatccgg cactctatca actgaaaatt
    22261 cagtattttc ttctttcctc aagctgatta ttagcctgga atagagagta gagggaactc
    22321 ccgatcggga gtgacgtaat ttgaggaata atgatgaaaa aaattgccat cacctgtgca
    22381 ttactctcaa gcttagtagc aagcagcgtt tgggctgatg ccgcaagcga tctgaaaagc
    22441 cgcctggata aagtcagcag cttccacgcc agcttcacac aaaaagtgac tgacggtagc
    22501 ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga aacgtccaaa cttattcaac
    22561 tggcatatga cacaacctga tgaaagcatt ctggtttctg acggtaaaac actgtggttc
    22621 tataacccgt tcgttgagca agctacggca acctggctga aagatgccac cggtaatacg
    22681 ccgtttatgc tgattgcccg caaccagtcc agcgactggc agcagtacaa tatcaaacag
    22741 aatggcgatg actttgtcct gacgccgaaa gccagcaatg gcaatctgaa gcagttcacc
    22801 attaacgtgg gacgtgatgg cacaatccat cagtttagcg cggtggagca ggacgatcag
    22861 cgcagcagtt atcaactgaa atcccagcaa aatggggctg tggatgcagc gaaatttacc
    22921 ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta agtagaggca cctgagtgag
    22981 caatctgtcg ctcgattttt cggataatac ttttcaacct ctggccgcgc gtatgcggcc
    23041 agaaaattta gcacagtata tcggccagca acatttgctg gctgcgggga agccgttgcc
    23101 gcgcgctatc gaagccgggc atttacattc tatgatcctc tgggggccac cgggtaccgg
    23161 caaaacaacc ctcgctgaag tgattgcccg ctatgcgaac gctgatgtgg aacgtatttc
    23221 tgccgtcacc tctggcgtga aagaaattcg cgaggcgatc gagcgcgccc ggcaaaaccg
    23281 caatgcaggt cgccgcacta ttctttttgt tgacgaagtt caccgtttca acaaaagcca
    23341 gcaggatgca tttctgccac atattgaaga cggcaccatc acttttattg gcgcaaccac
    23401 tgaaaacccg tcgtttgagc ttaattcggc actgctttcc cgtgcccgtg tctatctgtt
    23461 gaaatccctg agtacagagg atattgagca agtactaact caggcgatgg aagacaaaac
    23521 gcgtggctat ggtggtcagg atattgttct gccagatgaa acacgacgcg ccattgctga
    23581 actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg gaaatgatgg cggatatggc
    23641 cgaagtcaat gatagcggta agcgggtcct gaagcctgaa ttattgaccg aaatcgccgg
    23701 tgagcgaagc gcccgctttg ataacaaagg cgatcgcttt tacgatctga tttccgcact
    23761 gcataagtcg gtacgtggta gcgcacccga tgcggcgctg tactggtatg cgcgaattat
    23821 taccgccggt ggcgatccgt tatatgtcgc gcgtcgctgt ctggcgatag cgtctgaaga
    23881 tgtgggtaat gctgatccac gagcgatgca ggtggcaatt gcggcctggg attgctttac
    23941 tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag gcgattgttt acctggcctg
    24001 cgcgccaaaa agcaacgctg tctacactgc gtttaaagcc gcgctggcgg atgctcgcga
    24061 acgtccggat tatgacgtgc cggttcattt gcgtaatgcg ccgacgaaat taatgaagga
    24121 aatgggctac gggcaggaat atcgttacgc tcatgatgaa gcaaacgctt atgctgccgg
    24181 tgaggtttac ttcccgccgg aaatagcaca aacacgctat tatttcccga caaacagggg
    24241 ccttgaaggc aagattggcg aaaagctcgc ctggctggct gaacaggatc aaaatagccc
    24301 cataaaacgc taccgttaat gttatcgttg cggtaatgtt gttactgtat ccctgtggtc
    24361 gcaggctgtg gccacatctc ccatttaatt cgataagcac aggataagca tgctcgatcc
    24421 caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa ctggcacgcc ggggctttaa
    24481 gctggatgta gataagctgg gcgctcttga agagcgtcgt aaagtattgc aggtcaaaac
    24541 ggaaaacctg caagcggagc gtaactcccg atcgaaatcc attggccagg cgaaagcgcg
    24601 cggggaagat atcgagcctt tacgtctgga agtgaacaaa ctgggcgaag agctggatgc
    24661 agcaaaagcc gagctggatg ctttacaggc tgaaattcgc gatatcgcgc taaccatccc
    24721 taacttgcct gcagatgaag tgccggtagg taaagacgaa aatgacaacg ttgaagtcag
    24781 ccgctggggc accccgcgtg agtttgactt tgaagttcgt gaccacgtga cgctgggtga
    24841 aatgcactct ggcctcgact ttgcagccgc agttaagctg acaggttcgc gctttgtggt
    24901 gatgaaaggg cagattgctc gcatgcaccg cgcactgtcg cagtttatgc tggatctgca
    24961 taccgaacag catggctaca gtgagaacta tgttccgtac ctggttaacc aggacacgct
    25021 gtacggtacg ggtcagctgc cgaaatttgc tggcgatctg ttccatactc gtccgctgga
    25081 agaagaagca gacaccagta actatgcgct gatcccaacg gcagaagttc cgctgaccaa
    25141 cctggtacgc ggtgaaatca ttgatgaaga tgatctgcca attaagatga ccgcccacac
    25201 cccatgcttc cgttctgaag ccggttcata tggtcgtgac acccgtggtc tgatccgtat
    25261 gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc ccggaagact caatggcggc
    25321 gctggaagag atgactggtc atgcagaaaa agtcctgcaa ctgctgggcc taccgtaccg
    25381 taaaattatc ctttgtaccg gcgacatggg ctttggcgct tgcaaaactt acgacctgga
    25441 agtatggatc ccggcacaga acacctaccg cgagatctct tcctgctcca acgtttggga
    25501 tttccaggca cgtcgtatgc aggcacgttg tcgcagcaag tcggacaaga aaacccgtct
    25561 ggttcatacc ctgaacggtt ctggtctggc tgttggtcgt acgctggttg cggttatgga
    25621 aaactatcag caggctgatg gtcgtattga agtaccagaa gttctgcgtc cgtatatgaa
    25681 cggactggaa tatattggct aatacccaat ttttctgaat ctaaaaagcg cctgcgggcg
    25741 ctttttttgt ctccctttga taccgaacaa taattactcc tcacttacac gtaatactac
    25801 tttcgagtga aaatctacct atctctttga ttttcaaatt attcgatata tacaagccta
    25861 tatagcgaac tgctatagaa ataattacac aatacggttt gttactggaa tcaatcgtga
    25921 gcaagcttga gtgagccatt atgaaaacga aaatccctga tgcggtattg gctgctgagg
    25981 tgagtcgccg tggtttggta aaaacgacag cgatcggcgg cctggcaatg gccagcagcg
    26041 cattaacatt accttttagt cggattgcgc acgctgtcga tagcgccatt ccaacaaaat
    26101 cagacgaaaa ggttatctgg agcgcctgta cagttaactg tggtagtcgc tgcccgctac
    26161 gtatgcacgt cgtggacggt gaaatcaaat atgtcgaaac ggacaatacc ggcgatgaca
    26221 attacgacgg cctgcaccag gttcgcgcct gtctgcgtgg gcgttccatg cgtcgccgtg
    26281 tctacaatcc ggaccgcctg aaatatccga tgaaacgagt cggggcgcgc ggtgaaggca
    26341 aattcgagcg cattagctgg gaagaagcct acgacatcat cgcgaccaat atgcagcgcc
    26401 tgatcaaaga gtacggcaac gagtctatct atctgaacta tggcaccggt acgctgggcg
    26461 gcaccatgac ccgctcctgg ccgccgggaa ataccctggt cgcgcgcctg atgaactgct
    26521 gcggcggtta tcttaaccat tacggtgact actcttctgc gcaaattgcg gaaggtttga
    26581 actataccta cggcggctgg gcagatggta acagcccgtc ggatatcgaa aacagtaaac
    26641 tggtagtgct gtttggtaat aaccctggcg aaacgcgaat gagtggcggt ggggtgactt
    26701 actatcttga acaggcacgc cagaaatcta atgcccgcat gatcatcatc gatccgcgct
    26761 ataccgacac cggtgccggg cgcgaagatg agtggatccc tattcgtccg ggaacagatg
    26821 ccgcactggt taacggtctg gcgtacgtca tgatcactga aaacctggtg gatcaggcat
    26881 tcctcgataa atattgcgtt ggctacgatg agaaaaccct gccagccagt gcgccgaaaa
    26941 atggccacta taaagcttat attctgggtg aagggccaga tggcgtggct aaaacaccgg
    27001 aatgggcctc acaaatcacc ggtgttccgg cagacaaaat catcaaactg gctcgtgaaa
    27061 tcggcagtac caaaccggcg tttatcagcc agggatgggg cccgcagcgt cacgctaacg
    27121 gtgaaatcgc aacccgtgct atctcgatgc tggcgattct gaccggtaac gttggtatta
    27181 acggaggcaa cagcggcgcg cgtgaaggtt catacagctt accgtttgtc cgtatgccga
    27241 ccttggaaaa cccgatccag accagcattt cgatgtttat gtggaccgat gccattgaac
    27301 gtggcccgga aatgacggcg ctgcgtgatg gtgtacgcgg gaaagataag ctggatgtgc
    27361 cgatcaaaat gatctggaac tatgccggta actgcctgat taaccagcat tctgaaatca
    27421 accgtaccca tgaaatcctt caggatgata agaagtgcga gctgattgtg gttatcgact
    27481 gccacatgac ctcatcggcg aaatatgctg acatcctgct gcctgactgc accgcttccg
    27541 aacagatgga ctttgcgctg gatgcatcct gcgggaatat gtcttacgtg attttcaacg
    27601 atcaggtgat taaaccgcgc tttgaatgta agaccatcta tgaaatgacc agcgaactgg
    27661 caaaacgtct tggcgttgag caacagttta ctgaaggccg tacacaggaa gagtggatgc
    27721 ggcatctgta tgcccagtcg cgggaagcga ttcctgaact gccaacgttt gaagagttcc
    27781 gcaagcaggg gatctttaaa aagcgcgacc cacaagggca tcacgttgct tataaagcct
    27841 tccgtgaaga tccgcaggca aacccactga ctacgccatc gggcaaaatt gagatttatt
    27901 cgcaggcgct ggctgacatt gccgctacct gggaattgcc agaaggcgat gtgatcgatc
    27961 cactgccgat ctacacgccg ggctttgaaa gttatcagga tccgctgaac aaacagtatc
    28021 cgctgcagct tacgggtttc cactataaat ctcgcgttca ctcaacttac ggcaacgttg
    28081 atgtgctgaa agcggcttgc cgtcaggaaa tgtggatcaa cccgcttgat gcgcaaaaac
    28141 gcggtatcaa caacggcgat aaagtcagga tctttaacga tcgtggtgaa gttcatattg
    28201 aggcgaaagt gacgccacga atgatgccgg gtgtggtcgc actgggtgaa ggtgcctggt
    28261 atgacccgga tgcaaaacgt gtcgataagg gcggttgtat taacgtactg accactcaac
    28321 gtccgtctcc tctcgctaag gggaatccgt cacatacaaa ccttgttcag gttgaaaagg
    28381 tgtaaggagt aaccgatgac aacccagtat ggatttttta ttgattccag ccgttgcacc
    28441 ggttgcaaaa cctgcgagct ggcctgtaaa gactacaaag atttgacgcc agaagtcagc
    28501 ttccgccgca tttatgaata tgctggcggc gactggcagg aagataacgg tgtctggcac
    28561 cagaacgtgt ttgcctacta tctgtcgatt tcatgtaacc actgcgaaga tccggcttgt
    28621 actaaagtct gcccgagcgg tgcgatgcat aaacgcgaag atggttttgt tgtggtcgat
    28681 gaagatgtat gcattggctg ccgctactgt catatggctt gcccgtatgg cgcactgcaa
    28741 tataacgaaa cgaaaggcca tatgaccaaa tgcgatggtt gttatgaccg tgttgcggag
    28801 ggtaaaaagc cgatctgtgt tgaatcttgc ccgctgcggg cgctggattt cggtcctatc
    28861 gacgagctgc gtaaaaaaca tggcgatctg gcggccgttg cgccgttgcc gcgagctcac
    28921 tttaccaaac cgaatattgt gatcaaaccc aatgccaaca gccgcccgac cggggatacc
    28981 actggctacc tggcaaaccc gaaggaggtg tgagatggga agtggatggc atgaatggcc
    29041 gctgatgatc ttcacggtct tcgggcaatg tgtagcgggt ggttttatcg ttctggcttt
    29101 ggcgctgctc aaaggcgacc tgcgagcaga agcccagcag cgtgttatcg cctgcatgtt
    29161 tggcttatgg gtgctgatgg gcattggctt tatcgcctct atgctccatc ttggttcacc
    29221 gatgcgcgcc tttaactcgc tcaaccgggt aggggcttca gcactcagta acgaaatcgc
    29281 cagcggttcg atcttttttg ctgtaggcgg catcggctgg ttgctggcaa tgctgaaaaa
    29341 gctgtcaccg gcattgcgta cgctatggct ggtggtgacg atggttcttg gcgtcatctt
    29401 tgtctggatg atggtgcgtg tgtataacag cattgatacc gttccgacct ggtacagcat
    29461 ctggacgccg atgggcttct tcctgacgat gtttatgggc ggcccgttac tgggttacct
    29521 gttgttgagt ctggctggcg tcgatggctg ggcgatgcgt ctgctgccag ccatttctgt
    29581 actggcactg gtggtgagtg gcgtgatgtc agtgatgcag ggcacagagc tggcaactat
    29641 tcatagttct gtgcagcagg ccgcagcgct ggtgccggac tatggtgcgc tgatgtcctg
    29701 gcggatcgtg cttttggccg ttgccctgtg cttgtggatt gcaccacagc taaaaggtta
    29761 tcagcctgcg gttccgctac tatccgtctc attcattctg cttctggcag gggaattaat
    29821 cggtcgcggc gtattctatg gtttgcatat gaccgtgggg atggccgtcg caagctaatc
    29881 agtaacaacc ggggtttcgg ccccggttct cttttatttc tgcttcgtta acgtgtcata
    29941 actggtcatc aaattacgat aatcaggaat atggttagag aacaacgtcg ccagtccttc
    30001 aatatcatta cgccagtcac ggtgcagctc gcacgccacg ccgaaccatg tcatcaattg
    30061 cgcacctgct tgcgacatgc gatcccatgc cgaatgccgg gtaatttcat taaaggtccc
    30121 tgacgcgtca gtcacaacaa agacatcaaa cccttcttca atggctgaaa gcgccgggaa
    30181 tgccacgcaa acttcggtta ccacaccggc aataattaac tgttttttac ctgtcgcttt
    30241 gactgctttt acaaaatctt cgttatccca ggcgttaata tttcccgggc gagcaatgta
    30301 aggtgcatcg gggaattgtg ctttcagttc tggaactaat ggaccgttag gaccggtttc
    30361 aaaactggtg gtaagaatag taggtaagtt gaaatactta gccaggtcac ccagcgccag
    30421 cacgttattt ttaaacttat cgggttcgat atcccgtaca agggaaagta aaccagcctg
    30481 atgatcaaca agcaaaacgg cagcatcatt tttatcaaga cgaacatacg gtttggtcat
    30541 cctcttctcc tttcgagagt cagtggtacg tctgaaatcg gtcagacgtg atgcattgct
    30601 ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat gaaatttttc gactgaacga
    30661 agcgtatgaa atttgtgtta gatcaataaa aacaatcaga tgagattaat catctcttca
    30721 gcattagcgt tatgaatgag taatataaat cgataatacc gccgctgcga tatgcgcgta
    30781 ttttcaccca ttgacaatgt ttttggcggt ggcatgatgc gcatgaaatt tgaacttcct
    30841 cacggtttta attcatgtcc acgtataccc ggcctgtcat gcttttgctg tctggcctgc
    30901 ttttgttgac tctggcgatc gcggtgttaa atacactcgt gccgctttgg ctcgcccagg
    30961 aacacatgtc cacatggcag gtaggcgttg tcagctcatc ctattttacc ggcaaccttg
    31021 tcggtacatt gctgacaggg tatgtcatta aacgcattgg ctttaaccgc agctattatc
    31081 tggcctcctt catttttgcc gctggctgtg ccggccttgg cctgatgatt ggtttctgga
    31141 gctggttggc gtggcgtttt gtcgctggcg tcggctgtgc catgatttgg gtggttgttg
    31201 agagcgcgct gatgtgcagt gggacgtcac gtaaccgtgg gcgtttgctt gctgcataca
    31261 tgatggttta ttacgtggga acgtttttag gccagttact ggtcagcaag gtctcaaccg
    31321 agctgatgag cgtattgccg tgggttacag gtttgacatt ggcagggatc ttaccgctgt
    31381 tgtttacgcg tgtgctgaat cagcaggctg aaaaccatga ttcgacgtca attacggcaa
    31441 tgctaaaact ccgtcaggcg cggcttggcg tgaatggctg cattatctca ggaatcgttc
    31501 tgggatctct atatggcctg atgccgttgt acctcaatca caaaggggtg agcaatgcca
    31561 gtattggttt ctggatggcg gtactggtca gtgcgggtat cctcggacaa tggccgattg
    31621 gacgtctggc ggataagttt ggtcgactgc tggtgttgcg tgttcaggtc tttgtcgtca
    31681 ttctcggcag tatcgcgatg cttagccagg cggcgatggc ccctgcgtta ttcatcctcg
    31741 gtgccgctgg ctttacgcta tatccggtgg cgatggcctg ggcttgtgag aaagttgaac
    31801 atcatcaact ggtggcgatg aaccaggcct tactgttgag ctataccgtg ggaagtctgc
    31861 ttggcccgtc atttaccgct atgctaatgc agaatttctc cgataattta ttgtttatca
    31921 tgatcgccag cgtatcgttt atctatttgc tgatgctgct gcgcaacgcc ggtcatacgc
    31981 cgaaacccgt tgctcacgtg taaatgaatt caagcagagt gtgaacttac tgtttcacac
    32041 tctgcttttt tgtttcttct atctgacttg ctttattcca aattttattc gtttaaaaat
    32101 aaaatgtgca gcaggttata attttgcatt tcgcgatttc cgcacttctt atttgccgcg
    32161 cataatccct cgttttacag atgccccttt aattttggcg aaggatttgt ctatggctgg
    32221 gaatgttcag gaaaaacagt tgcgatggta caacattgcg ctgatgtctt ttatcactgt
    32281 ctggggtttt ggcaacgttg ttaataacta tgccaaccag gggctggtgg ttgttttttc
    32341 atgggtgttt atctttgcac tctatttcac accttatgcg ctaattgttg gtcagttagg
    32401 ctcgaccttc aaagatggga agggcggggt cagtacctgg attaaacaca cgatgggacc
    32461 cggactggct tatctcgccg cgtggaccta ctgggtggtg catattccct atctggcaca
    32521 aaaaccccag gcaattctga ttgcgctcgg ttgggcgatg aaaggcgacg gttcgctaat
    32581 caaagaatat tcagtcgtag cgttacaggg gttaacgctg gtgctgttta tcttctttat
    32641 gtgggttgct tcacgcggta tgaaatcgct gaaaatcgtc ggttctgtgg cagggattgc
    32701 gatgtttgtt atgtcgctcc tgtatgtggc gatggcggta accgcgcctg cgattactga
    32761 agtgcatatt gcgaccacaa acattacctg ggaaacgttc attcctcata tcgactttac
    32821 ctacattacc actatttcaa tgctggtttt cgcggttggc ggagcagaga agatttctcc
    32881 ttacgttaat caaacgcgca acccaggaaa agaatttcca aaagggatgc tatgcctggc
    32941 ggtgatggtt gcggtttgtg ccattctggg ctcactggcg atggggatga tgtttgactc
    33001 gcgtaatatc ccggatgact taatgacgaa cggtcaatat tacgcctttc agaagctggg
    33061 cgagtattac aacatgggta atactttaat ggtgatttac gccattgcga ataccctggg
    33121 acaagtagct gcgctggtat tctcgattga tgccccgctt aaagtgctat taggtgatgc
    33181 tgacagcaaa tatattccag ccagtttatg tcgtaccaac gcttctggta cgcccgttaa
    33241 tggctatttt ctgaccctgg tgctggtggc aattctgatt atgctgccga ctctcggcat
    33301 cggtgatatg aacaatctct ataaatggct gttgaatctt aactcggtag tgatgccgct
    33361 gcgttattta tgggtatttg ttgcatttat tgcagtcgtt cgcctggcgc agaaatataa
    33421 accagaatat gtctttattc gtaataagcc tctggcaatg accgtcggga tttggtgttt
    33481 tgcctttacc gcctttgcct gtttgacggg gatcttcccg aaaatggaag ccttcactgc
    33541 agagtggacc ttccagttgg cgctgaatgt tgcaacgccg tttgtgctgg taggattagg
    33601 actgattttc ccgctgctgg cgcgtaaagc gaatagtaaa taattattgt ggtcggtgac
    33661 gctgactctg gcagatctga cccgttttta cataatcgag aaatgcgcgc aagccgcagg
    33721 acatatactg gcggtttgga taatagatct ggaagcctgg acgctctgtg ctccagtctc
    33781 ccagtacaca ttctaaacga ccagtatcaa ggtactcctt gatctgttca tagagcagat
    33841 acccgatacc agcacccatc aaaacggcct ccagttcagc atcaacatca tccagaatga
    33901 tatttcctgc gacagcaatt tccagctcct ttgcaaattg ccagtggaaa ggtttgccgc
    33961 tgggataacg aaaaacaaca cattgatgat taagcagatc atgaggatgg cggggtttgc
    34021 catatcttgc aaagtactct ggcgttgctg ctacacataa tttcactggt gggccgatcg
    34081 ccacgctgat catatctttt tcgacgatgc cactaagtcg aacgccagcg tcaaagccct
    34141 gttggacgat atcgacaaga gaatcgtcag ttgtgagctc gactttaata tcaggatatt
    34201 cacgtgtgaa accaaccagt aaaggcatta aaaatatacg tgcagctacc cgggcggcat
    34261 tgattttgag cgttcctgtc ggtgtcagac gaaaatcatt catctcatct aacatgatct
    34321 gaatttcgtc gaaagctggg cgcaatcgtt catagagatt agaacccgct tccgttaacg
    34381 atacgcttcg ggttgtccga ttgaataagc gaattttaag acgttgttcc agtgttttaa
    34441 tactatggct aatggcggac gaggataagc ctaactcatc gcccgctgca cgaaagcttt
    34501 gattacgggc gacggcaaag aaagtggcaa agtcagacat attcatccgc attagtgaaa
    34561 atccttcatt aactcatcct gaatcatcac gtttacagga ttatgccgcc tgcgtaaagt
    34621 gcgctccaga acttaacgtg gaggtaaaat tatgcagtct gaacgtattt atttggtatg
    34681 ggcccatcct cgtcatgatt cattgaccgc aaatattgct gatgcgatcc atcagcgggc
    34741 aatggagcgg aaaatacagg tgacggaact cgatttatat cggcgtaatt tcaacccagt
    34801 gatgacgccg gaagatgaac cagactggaa gaatatggat aaacgttatt ctccagaggt
    34861 tcatcagctt tattcagagc tgcttgaaca tgacacgtta gtggtggttt ttcctctctg
    34921 gtggtacagc ttcccggcaa tgctaaaagg atatattgac agagtatgga ataatgggct
    34981 ggcttatgga gatgggcaca aattaccatt caataaagtt cgttgggtgg cgctggttgg
    35041 aggagacaaa gaatcatttg tccagatggg ctgggaaaaa aatataagcg attatttgaa
    35101 aaatatgtgc agttatcttg gtattgaaga tgccgatgtc actttcttgt gtaatacagt
    35161 ggtattcgat gggcaagaac ttcatgcgag ctattatcag tcgttattat ctcaggtacg
    35221 ggatatggta gatgcactat aaaatgtgtt aaaaacgctg tagcagaatg aagcgcggaa
    35281 taaaaaagcg gcaactcaat aaagttgccg ctttacgggg aaattagaac attaccttat
    35341 gaccgtactg ctcaagaatg cctttcacgc gttccatggt ctctttctta ggtggtttaa
    35401 caccgtcgag tttgtactct tcacccattg ccacccattt gtgtttgccc agctcgtggt
    35461 aggggaggag ttcgattttc tcaacgttgc ccatatcacg ggtaaattca ccgaggcgat
    35521 gcgctgaatc gtcatcgtca gaccagcctg ggacaacaac gtagcggatc cacaccttca
    35581 catttttgtt cgccagatat ttggcgaact ccagcgtgcg gtggttggaa actccaacca
    35641 gattttggtg gatctcgtcg ttcatctgtt tgagatcgag cattaccagg tcggttactt
    35701 ccagcagttc atcaatcacc ggatcgtaac gacgaacaaa accgttggtg tccagacagg
    35761 tatgaatgcc ttcttttttg caggcgcgga accagtcacg aacaaactca gcttgcagga
    35821 ttgcttcacc gccggatgcg gtaacgccgc cgccggaagc gttcataaag tggcgatagg
    35881 tcaccacttc cttcatcaaa tcttcaacgg taacttcttt accgccatgc gtgtcccagg
    35941 tgtcgcggtt atgacaatac aggcagcgca tcaggcagcc ctggaaaaag gtgataaagc
    36001 gaatacctgg gccgtctacg gttccacagg attcaaagga gtgaatgcga ccaataactg
    36061 acattgcggt gtttctccag atgtggccca tctgaggccg tgttggtgcg cagctcgaag
    36121 gctacgtcga gtctgttttg gcagtcacct taaagtatag atagctgaca aaaaaggctc
    36181 tcgtgctaaa aaaggcccca ctttcgtgga gcctttattg tacgcttttt actgtacgat
    36241 ttcagtcaaa tctaattaca tagattgagt gaaggtacga gtaataacgt cctgctgctg
    36301 ttctttagtc agcgagttga aacgtactgc gtagccagat acacggatgg tcagctgcgg
    36361 atatttttcc gggttttcca tcgcgtcgag cagcatttca cggttcatca cgttaacgtt
    36421 caggtgctga ccaccttcga tggatgcttc gtggtggaag taaccatcca tcagaccagc
    36481 caggttggtc ttacgaactt cgtcgtcttt acccagtgcg ttcggaacga tagagaaggt
    36541 gtaggagata ccatctttag cgtaagcaaa cggtagttta gcaacggaag tcagagacgc
    36601 tacagcacct ttctggtcac gaccgtgcat cgggttagca cccggtccga acggcgcgcc
    36661 agcacgacga ccgtctgggg tgttaccagt tttcttacca tacacaacgt tagaagtgat
    36721 ggtcagaaca gactgagtcg ggatagcgtc acggtaggtg tgcagtttct gaattttctt
    36781 catgaaacgt tctaccaggt caacagccag gtcatctaca cgcggatcat tgttaccaaa
    36841 ctgcgggtat tcgccttcga tttcgaagtc gatagcaaga ccgtcttcgt cacgaatcgg
    36901 tttaactttc gcatatttga ttgcagacag ggagtcagca gcaacggaca gaccagcgat
    36961 accacacgcc atggtgcgga taacgtcacg gtcgtgcagc gccatcagag aggcttcgta
    37021 gctgtacttg tcgtgcatgt agtggatgat gttcagtgca gtgatgtact gtttagccag
    37081 ccagtccatg aagtgatcca tgcgctccat cacttcatcg tagttcagga gatcgccttt
    37141 gatcggttca gacttcggac caacctgcat tttcagtttt tcgtcaacgc cgccgttgat
    37201 tgcgtacagc atggttttcg ccaggtttgc acgcgcaccg aagaactgca tttgtttacc
    37261 aacgatcatc gggcttacgc agcaagcgat agcatagtca tcgttgttga agtccggacg
    37321 catcaggtca tcgttctcat actgcagaga agaggtgtcg atggacactt tagcggcgaa
    37381 tttcttgaag ttcagcggca gtttttcaga ccacagaatg gtcatgttcg gttccggaga
    37441 cggacccatg gtgtacaggg tgttcaggaa acggaagctg tttttggtaa ccagggtacg
    37501 accgtcgagg cccataccac cgatagattc ggttgcccag atcgggtcgc cagagaacag
    37561 ttcatcgtat tccggagtac gcaggaagcg aaccatacgc agtttcatga ccaggtggtc
    37621 aaccatttcc tgcgcttctt gttcggtgat cttgccagct ttcaggtcac gttcgatgta
    37681 cacatccagg aaggtggagg tacgaccgaa ggacattgca gcaccgttct gagacttaac
    37741 agcagccagg tagccgaagt aagtccactg gatagcttcc tgagcgttgg tagccggacc
    37801 agagatgtcg tagccgtatt tcgcagccat ttctttcatc tgacccagag cgcggtgctg
    37861 ttcagcgatt tcttcgcgca gacggatagt ctgttccagg tttacgccgt tttccagatc
    37921 agcctgcaga gaagtgaact gtgccagttt gtctttcatc aggtagtcga taccgtacag
    37981 cgcaacgcga cggtagtcac cgatgatacg gccacggcca tatgcatctg gcagaccggt
    38041 cagaacacca gatttacggc aacgcaggat gtccggagtg taaacgtcga acacgccctg
    38101 gttgtgagtt ttacggtatt cagtgaagat ttttttgatc atcggatcca gttcgcggtt
    38161 gtacgctttg caggaacctt cgatcatttt gataccaccg aacgggataa gagcacgttt
    38221 cagcggagct tcagtctgca gaccaacgat tttctcaagc tgcttgttga tgtagccagc
    38281 gtcgtgagag gtgatggtgg aagcaacagc ggtgtcaaag tcaactggcg cgtgagtgcg
    38341 gttttccagt ttaacgcctt ccattacttt gtcccacagg gtggtggtcg cttcagtagc
    38401 gccagccagg aaggactcgt caccctcgta cggagtgtag tttttctgaa tgaagtcacg
    38461 gacgtttact tcattctgcc agtcaccttt ggtaaaacct tcccaggctg tggctaactt
    38521 ttcattaagc tcggacatgt aacacctacc ttcttaagtg gattttttat ttactgcgta
    38581 cttcgacaac cattaatggt gatcgttttc acgcaggtaa atgacccagt atgtcaaccc
    38641 aaccaacaaa ccaccgccga taatattacc gatcgtaacc ggaatcaggt tatcagtgat
    38701 gaagttcatc acggtcaggt gagaaaaatt ttccggtgca gaaccgacag cggtccagaa
    38761 ttccggagat gcgaagtcgc ggattacaat acccatcggg atcataaaca tgtttgcgat
    38821 actgtgctca aaaccgctgg caacaaacat cgcgaccggc agcaccataa tgaacgcttt
    38881 gtccatcagg ctacggccag aataactcat ccatactgcc agacatacca tcaggtttgc
    38941 caggatacca aggcagacgg cctcaataaa agtatggtgc actttgtggt cggcggtttg
    39001 taggacgttt agtccccatt gaccatttgc ggtcatatac tcgccggaaa gccacattaa
    39061 aagtacaaac agcagtgcgc cgaccaggtt gccaaaatag acatttagcc agtttttcgc
    39121 caactgaccc caggtgatgc gcccactcgc cttagcaaca acaatcaaca ctgtggaagt
    39181 aaagagatcg gctccgcaga caacacaaag aatcagcccc agagagaagc aaatgccgcc
    39241 aaccagtttt gccatgccga agggcattgt gcctgtgcca gtggttgctg tgatatagaa
    39301 gacgaatgcg attgagatga aaacaccggc ggtaatcgcc agatagaaag tcttaagcgg
    39361 atgtttcgtt gctttataga cacccgcctc ttcggccact ttggccattg cagcaggaag
    39421 taaaagatca aaagggttgt cagctttcac actaactctc tctttattaa gtcggcgacg
    39481 agatactaac aaagcattat agatgagaaa ttgatataga tcatatctcg cctggcttat
    39541 aggcccgtaa ctcgcatggt ttttatgcaa atacggagta aatatttgat tatccaaata
    39601 aaaataaatt ttaaaaatta acaaatgagt tgaatttttt ccgcatcctc cgctaaaaca
    39661 gttaattaaa aggga
//
LOCUS       DAZFRN010000030        38436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFRN010000030 DAZFRN010000000
VERSION     DAZFRN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.030"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            80..2323
                     /gene="pdeF"
                     /locus_tag="ACHKUB_001486"
     CDS             80..2323
                     /gene="pdeF"
                     /locus_tag="ACHKUB_001486"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO6654523.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2362..3903)
                     /gene="ppx"
                     /locus_tag="ACHKUB_001487"
     CDS             complement(2362..3903)
                     /gene="ppx"
                     /locus_tag="ACHKUB_001487"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO6654524.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3908..5974)
                     /gene="ppk1"
                     /locus_tag="ACHKUB_001488"
     CDS             complement(3908..5974)
                     /gene="ppk1"
                     /locus_tag="ACHKUB_001488"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO6654525.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6145..6783)
                     /gene="purN"
                     /locus_tag="ACHKUB_001489"
     CDS             complement(6145..6783)
                     /gene="purN"
                     /locus_tag="ACHKUB_001489"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO6654526.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6783..7820)
                     /gene="purM"
                     /locus_tag="ACHKUB_001490"
     CDS             complement(6783..7820)
                     /gene="purM"
                     /locus_tag="ACHKUB_001490"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO6654527.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8145..8771
                     /gene="upp"
                     /locus_tag="ACHKUB_001491"
     CDS             8145..8771
                     /gene="upp"
                     /locus_tag="ACHKUB_001491"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO6654528.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8857..10146
                     /gene="uraA"
                     /locus_tag="ACHKUB_001492"
     CDS             8857..10146
                     /gene="uraA"
                     /locus_tag="ACHKUB_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO6654529.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTMI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10241..10942
                     /gene="hda"
                     /locus_tag="ACHKUB_001493"
     CDS             10241..10942
                     /gene="hda"
                     /locus_tag="ACHKUB_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO6654530.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11080..11439)
                     /gene="arsC"
                     /locus_tag="ACHKUB_001494"
     CDS             complement(11080..11439)
                     /gene="arsC"
                     /locus_tag="ACHKUB_001494"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO6654531.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVESEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11460..12923)
                     /gene="bepA"
                     /locus_tag="ACHKUB_001495"
     CDS             complement(11460..12923)
                     /gene="bepA"
                     /locus_tag="ACHKUB_001495"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO6654532.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13136..14197
                     /gene="yfgO"
                     /locus_tag="ACHKUB_001496"
     CDS             13136..14197
                     /gene="yfgO"
                     /locus_tag="ACHKUB_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6654533.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14235..15083)
                     /gene="focB"
                     /locus_tag="ACHKUB_001497"
     CDS             complement(14235..15083)
                     /gene="focB"
                     /locus_tag="ACHKUB_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO6654534.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15105..17117)
                     /gene="hyfR"
                     /locus_tag="ACHKUB_001498"
     CDS             complement(15105..17117)
                     /gene="hyfR"
                     /locus_tag="ACHKUB_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO6654535.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNNLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17147..17560)
                     /gene="hyfJ"
                     /locus_tag="ACHKUB_001499"
     CDS             complement(17147..17560)
                     /gene="hyfJ"
                     /locus_tag="ACHKUB_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO6654536.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17553..18311)
                     /gene="hyfI"
                     /locus_tag="ACHKUB_001500"
     CDS             complement(17553..18311)
                     /gene="hyfI"
                     /locus_tag="ACHKUB_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO6654537.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18308..18847)
                     /gene="hyfH"
                     /locus_tag="ACHKUB_001501"
     CDS             complement(18308..18847)
                     /gene="hyfH"
                     /locus_tag="ACHKUB_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO6654538.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            complement(18857..20572)
                     /gene="hyfG"
                     /locus_tag="ACHKUB_001502"
     CDS             complement(18857..20572)
                     /gene="hyfG"
                     /locus_tag="ACHKUB_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO6654539.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20562..22142)
                     /gene="hyfF"
                     /locus_tag="ACHKUB_001503"
     CDS             complement(20562..22142)
                     /gene="hyfF"
                     /locus_tag="ACHKUB_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO6654540.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22147..22797)
                     /gene="hyfE"
                     /locus_tag="ACHKUB_001504"
     CDS             complement(22147..22797)
                     /gene="hyfE"
                     /locus_tag="ACHKUB_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO6654541.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22809..24039)
                     /locus_tag="ACHKUB_001505"
                     /pseudo
     CDS             complement(22809..24039)
                     /locus_tag="ACHKUB_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(24056..25003)
                     /gene="hyfC"
                     /locus_tag="ACHKUB_001506"
     CDS             complement(24056..25003)
                     /gene="hyfC"
                     /locus_tag="ACHKUB_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO6654542.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25014..27032)
                     /gene="hyfB"
                     /locus_tag="ACHKUB_001507"
     CDS             complement(25014..27032)
                     /gene="hyfB"
                     /locus_tag="ACHKUB_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO6654543.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVAHFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     TLVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEDAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27032..27649)
                     /gene="hyfA"
                     /locus_tag="ACHKUB_001508"
     CDS             complement(27032..27649)
                     /gene="hyfA"
                     /locus_tag="ACHKUB_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO6654544.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(27902..28372)
                     /gene="bcp"
                     /locus_tag="ACHKUB_001509"
     CDS             complement(27902..28372)
                     /gene="bcp"
                     /locus_tag="ACHKUB_001509"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO6654545.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28372..28944)
                     /gene="gcvR"
                     /locus_tag="ACHKUB_001510"
     CDS             complement(28372..28944)
                     /gene="gcvR"
                     /locus_tag="ACHKUB_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO6654546.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29090..29968
                     /gene="dapA"
                     /locus_tag="ACHKUB_001511"
     CDS             29090..29968
                     /gene="dapA"
                     /locus_tag="ACHKUB_001511"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO6654547.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            29985..31019
                     /gene="bamC"
                     /locus_tag="ACHKUB_001512"
     CDS             29985..31019
                     /gene="bamC"
                     /locus_tag="ACHKUB_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO6654548.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31232..31945
                     /gene="purC"
                     /locus_tag="ACHKUB_001513"
     CDS             31232..31945
                     /gene="purC"
                     /locus_tag="ACHKUB_001513"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO6654549.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32099..32326
                     /gene="vapB"
                     /locus_tag="ACHKUB_001514"
     CDS             32099..32326
                     /gene="vapB"
                     /locus_tag="ACHKUB_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO6654550.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32326..32724
                     /locus_tag="ACHKUB_001515"
     CDS             32326..32724
                     /locus_tag="ACHKUB_001515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO6654551.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            32871..33734
                     /gene="ypfJ"
                     /locus_tag="ACHKUB_001516"
     CDS             32871..33734
                     /gene="ypfJ"
                     /locus_tag="ACHKUB_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO6654552.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33749..35764
                     /gene="tmcA"
                     /locus_tag="ACHKUB_001517"
     CDS             33749..35764
                     /gene="tmcA"
                     /locus_tag="ACHKUB_001517"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO6654553.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHVLKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALCALNDVRTERLRDRITQWQFFH"
     gene            35838..36536
                     /gene="ypfH"
                     /locus_tag="ACHKUB_001518"
     CDS             35838..36536
                     /gene="ypfH"
                     /locus_tag="ACHKUB_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6654554.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36646..36846)
                     /gene="ypfN"
                     /locus_tag="ACHKUB_001519"
     CDS             complement(36646..36846)
                     /gene="ypfN"
                     /locus_tag="ACHKUB_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO6654555.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36874..38001)
                     /gene="dapE"
                     /locus_tag="ACHKUB_001520"
     CDS             complement(36874..38001)
                     /gene="dapE"
                     /locus_tag="ACHKUB_001520"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO6654556.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38005..38361)
                     /gene="yffB"
                     /locus_tag="ACHKUB_001521"
     CDS             complement(38005..38361)
                     /gene="yffB"
                     /locus_tag="ACHKUB_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO6654557.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9343 a         9924 c        10110 g         9059 t
ORIGIN      
        1 ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat acacctcctt caggatgtgg
       61 gggattctgc tgagcatcta tgaaactgaa tgcaacttat ataaaaatac gtgataaatg
      121 gtgggggctt ccgctgttcc tgccttcttt aatcttgccc attttcgccc acattaatac
      181 tttcgcgcat atttcttccg gtgaggtttt tctcttttat ctgcctttgg cactgatgat
      241 cagcatgatg atgtttttca gctgggcggc attgccaggg atcaccttag ggatttttgt
      301 ccgcaaatat gcagagctgg gtttttacga aacgctctca ttaacggcta attttattat
      361 cattatcatt ctctgttggg gcggttacag ggtctttact ccccggcgta acaacgtttc
      421 acatggtgat acccgtttaa tttcccagcg tatattctgg cagattgtgt ttcctgcaac
      481 gctgtttctg atacttttcc agtttgctgc atttgtagga ttactggcga gcagagaaaa
      541 tctggtcggt gtcatgcctt ttaacctcgg gactttaatc aattatcagg ccttactggt
      601 gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt cgggtagtgc gaaatccatt
      661 ttatttacgt agctattatt cgcaattaaa acagcaggtt gatgccaaag tcaccaaaaa
      721 agagttcgcg ctctggctac tggcattagg tgctttattg ttgctgttat gcatgccgtt
      781 aaatgaaaaa agcacaattt ttagcaccaa ttataccttg tcattattgc tgcccctgat
      841 gatgtgggga gcgatgcgct atggttataa gctgatttcg ctgctctggg cggtcgtgtt
      901 gatgatcagc atccacagct atcaaaatta cattcccatt tatcctggct ataccacgca
      961 gctgaccata acctcctcca gttatctggt attctctttt attgtcaatt atatggctgt
     1021 actggcaact cgtcagcgag cggtagtcag acgcattcag cggcttgcgt atgtggaccc
     1081 ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg ttgcgtgatg ccccctggtc
     1141 tgcgctttgt tatttacgca tccctggcat ggaaatgctg gttaagaact atggcatcat
     1201 gctgcggatt caatacaagc aaaaactttc tcactggctg tcacccttgc tggaaccggg
     1261 tgaagatgtt tatcagcttt cgggtaacga tctcgcgctg cgactgaata cagaatcgca
     1321 ccaggagcgc attaccgcac tggatagcca tctcaagcaa tttcgtttct tttgggatgg
     1381 catgccgatg caaccgcaga ttggcgtcag ctactgctat gtgcgctcgc cagtgaatca
     1441 tatctacctg ctgctgggag agctaaatac ggtcgccgaa ctttccatcg tgaccaacgc
     1501 cccggaaaat atgcagcgtc gcggggcaat gtatttgcaa cgcgaattga aagataaagt
     1561 cgcgatgatg aatcgactac agcaggcgct ggaacacaac cattttttcc tgatggccca
     1621 gccgattacc ggtatgcgtg gtgatgttta ccatgaaatt cttctgcgca tgaaaggtga
     1681 gaatgatgaa ctgatcggcc ccgatagctt cttgccggtc gcgcacgaat ttggtttatc
     1741 gtcgagtatc gacatgtggg tcattgagca tacgctgcaa tttatggctg aaaacagagc
     1801 gaagatgccc gctcaccgtt ttgctattaa tctgtctcca acctcggtat gtcaggctcg
     1861 ttttcctgtt gaagtcagtc agttgctggc taaatatcag attgaagcgt ggcaacttat
     1921 ttttgaagtc accgaaagta atgctctgac caatgttaag caggcgcaaa tcaccttgca
     1981 gcatcttcag gaattaggct gccagattgc gattgatgat ttcggcaccg gctacgccag
     2041 ctatgcacgg cttaaaaatg tgaatgccga tctgcttaaa attgacggca gttttatccg
     2101 caatattgtg tcaaatagtc tggattatca gatagtggca tcgatttgcc acctggcgcg
     2161 aatgaagaaa atgcgggtag tggcagagta cgttgaaaac gaagagatcc gcgaggcggt
     2221 gctctctttg gggatcgatt atatgcaggg ttatcttatt ggtaagccgc agccgttaat
     2281 tgatacgttg aatgaaatcg aacccattcg cgaaagtgcc tgaataatgc gggccgacat
     2341 ttcttgtcgg cccgcaaagc attaagcggc gatttcaggt gtactttctt cttcaatttt
     2401 caaccgccag ccagccacgc cttcccagta ttcttgctcc ttttccagat caagcagtac
     2461 cagcgcattc tgactaaacc agtcatgcgg gaaacgcagt gtccagtgac tgtcatcagt
     2521 aatcagtgtc aatgttggcg gtgtggtggt tgcctgacgt tgattgttta gtaatacgcc
     2581 aaggcgcaat agctgtatca gtggcaggaa ctgtttcttc ttaaacaagg taaagcgcgg
     2641 cagatcgtcg agcttaatcg ctttacggtg atagcgcacc agtgtcgcca tcatcagctg
     2701 ctgttcctga ttaaaacccg gcaagtcact gttttgcaga atataagcgg agtggcgatg
     2761 caaaccgctg tggttgatat tcaacccgac ctcatgcagc atggcggccc atcgcagtag
     2821 cgcctccagt tgcggatgcg ccagcttcgg ttgctgttcc cgccactgtt cgtacatttg
     2881 catagtggta tccagcaccc gtcgggcctg ctcgctgtcg atgtgatact ggttggcgag
     2941 gctgctggcg gtgcgactac gcacatcctg atgacggaaa cgtccttcca tttcatacag
     3001 tacgccttcg cgtaaagccc cgtcagaaag gcgcagttca cggatggcta aagcatcaaa
     3061 cacaccgcat aaaatcgcca gtccaggaac gaagactgtt ttccgctctt cggaaagacc
     3121 cggtaaactc agcgatgcga aattacggtg acgtaaaact tcttttacca gtttttccag
     3181 acgttccggg gtaattatcc cgtctttctc gcccatttcc attaacactt catgggcggc
     3241 ttttatggta ccggaagcgc ccattgccac gttccagccc tgaatacgga attgccaggt
     3301 taaagtttcc agtttttgtg ctgccgccat acgagcgcgc tgaaaattct ctttattgat
     3361 gaccccgcca gggaagtaaa gctgggcaaa gctgacacaa cccatccggc ggctttcaac
     3421 gagaataggt tcgaaatttt caccaatcac cagttccgta gatccgccgc caatatcaat
     3481 aaccagtttg cgacctttct ccggttgggt atgttccacg cccataaaaa tcagacgggc
     3541 ttcttcatta ccggaaataa tttcaatcgg gtagggaatg accttttccg cgcgtttcag
     3601 aaagtcagtg gcgttcagcg cctgacgcag cgtatgggtg ccgactatac agacgctggc
     3661 aggagaaaac ccttgtagcc gttcggcaaa cagcgacaga cagttcaaac cgcgcgtcat
     3721 tgcctcttca ctcaacatat tatctggccc caggccgtcc gccagatgca cccgctgttt
     3781 caggcggcca ataatctgca tggcaccatc taccacacgg gctatgacca tgtgaaaact
     3841 gtttgaacca agatcgaccg cagcaaactc ctgcggacga ggggatttat cgtgtattgg
     3901 catagggtta ttcaggttgt tcgagtgatt tgatgtagtc gtaaatcgcc aactgcgccc
     3961 gtactttgcg gcgattgccg cggggaacgt agcgattact gagttcttta tcgatataac
     4021 gtgctttgac cgtatcgctg aacaatatgt cgatgatgtc cagcacccgc tgcttcaggc
     4081 gcggatcgag cagcggtgtc gccacttcaa tacgataatc aatattgcgc gtcatccagt
     4141 cggcggaaga aaggtagacc tttttatcgc cgccattttc aaaaatataa acccggtcat
     4201 gttctaggta acggtcaaca atactgatgg cacgaatgtt gtcgctaatg ccttccagat
     4261 tggggatcag cgaacacatt ccgcgaacca gcagattaac cggtacgccg gagctggagg
     4321 ccgcatacag acgatcaacc aggcctttat cgacaaggtt atttagcttc agggtgatac
     4381 cgctgggcag cccttgctgc gcgttggcga tttcgcggtc taccatttca tataacagac
     4441 ggcgagagtt ttgcggtgac accattaaat aatcaaatgt caccgggcgg tatgggtttt
     4501 caataaagtt aaatacccgc cgtacttcgt tggtgatacg cgcatcggcg gtcagcaatg
     4561 aatagtcagt ataaagacgc gcggtttttt cgttaaagtt cccggtcccg atgtgtgcgt
     4621 aacgcaccac ttcaccgttt tcttttcgtg aaatcaggaa cagtttggcg tgaattttca
     4681 gccccggcgc agagaagata acgtgcacgc ctgcttcggt caggcgcttc gcccagtgaa
     4741 tgttagcttc ttcgtcgaaa cgcgcctgta actcaaccac cacggtgact ttcttaccgt
     4801 tatgtgcggc gtggatcatc gagtcgatga tgcgtgaatc tttcgccacg cggtaaatat
     4861 taattttaat cgccagtacg ctcgggtcga acgaagcctg acgcagcagt tccagcacat
     4921 gctcaaaggt gtgataagga taatagagca acacatcgcg ttcgcgaatg gcatcaaaac
     4981 cattgcggaa ctgggcttta tcaaaccaaa tatggcgtaa acgcggcagt ggtttgttca
     5041 ccagattggc tttgccgaca ttggggaaat taataaagtc tttaaaatta tgataacgac
     5101 cgccggggac gatggagtcg tagcgggaaa tagtcagttt ttcgcgtaac acttcaacca
     5161 gcgcattggg catatcgcgc tgataaacaa aacgcaccgg ctcagcagtt aaacgctgct
     5221 tgagactgga agacatcaac tccatcaggc tggcttccat ctcatgcact aaatcgtatt
     5281 cggcatcgcg ggtcatcttc attgaatagg cattcagcgc gtcataatca aagaagcctt
     5341 tgaaaatatc atcaaggcag taacgcagaa tgttatccag aagaatcatc ggcttgcgtc
     5401 gacgcggcgc ttctggcggt aaattcacaa agcgcggcac tttatctgat gggatctcca
     5461 gcagcgcgta acggatggta tcgccacgga taatttccac cgccagatag gtgtaatcat
     5521 ctttcaggaa ctgcactaag tcagtgtcag gattgattaa aatcggcgta atgtgctgac
     5581 gcagatactg cttaaaataa tgacgcagcc agttttgttg attgacggag agctggcgtt
     5641 cattaatcag gaagatctgg ttacgcgcca tctccagcag cagctcgttg tagaggccgt
     5701 cgaattcctg atcggctttc agcacccggg actgaatttt gcccagtaaa tggcgggaat
     5761 gagagttgga gccttgttct tcgctaataa tgatgcgtcg cttcagttca gcgaagcgga
     5821 ctttatagaa ctcatcaagg ttattggaat agatccccag gaaacgcatc ctttcaatca
     5881 gcgggttaga tttgtccgcc gcttcctgaa gcacgcgttc attgaacgat aaccaactga
     5941 gctctttttc gatgtatagc ttttcctgac ccattaccac ttttactccg ttttattcac
     6001 gggacactgc ctggatatta tggcgagcaa tattggcaga tgtccaacta tgccagaaaa
     6061 gggggaaaaa aactgctgga gcttgcgatt tacaggtgaa ttgaaaaacg ccagcggcag
     6121 agctggcgct ttaactacgg ggcattactc gtcggcagcg tagccctgcg gcggcagacg
     6181 ttgaccatcc agccacgcgg cgttttcgtg cattttcaga cgaccatcgg caaaccagct
     6241 aatcaccagt ggataaatag cgtgttcctg ggtttgcacg cgggcggtga tgtcatcttc
     6301 cgtatcacca gcaaataccg ggactttcgc ctgtaaaata accgggccac cgtccagttc
     6361 atcggtgacg aaatgcaccg atgtaccgtg ctcttcatca ccattttcca gcgcctgacg
     6421 atgggtgtgt aatccgggat atttcggcag cagagaaggg tgaatgttca gcaaacgccc
     6481 ggcatagtgg gagacaaacg ccgggctgag aatgcgcata aaaccagcca gcacgaccac
     6541 atcgggtgcg tacatgtcga tttcatgaat caactcccgg tcataggctt cacgactgtc
     6601 aaacgcgctg gcgatgagcg tatgcgttgc aatacccgcc tggcgggcgc gttcaaggcc
     6661 gaacgcgtcg gccttattgc tgaaaactgc ccgtacggtg cctttaattt tgttagtttt
     6721 acaggcgtca ataattgcct gtaaattact tccgttgccg gaaataagca ccacaatatt
     6781 cattattcga taaccacgcg ttgttcggaa tcagaggctt tgatgatacc gattttccac
     6841 gcgttttcac cgttggagtt tagcagggcg agggctttgt ccacttccgg agcaggcagg
     6901 gcgataatca tcccgacgcc gcagttgaag gtgcgataca tttcatggcg ctcaacgtta
     6961 cctgccgttt gcagccagtt gaacacttcc ggccactgcc aggaagattc atcaatcact
     7021 gcctgagtat tatctggcaa tacgcgtgga atgttttccc agaagccgcc gccggtcagg
     7081 tgcgcaatgg catgcacatc gaccttttca atcaactcca gcactgactt cacgtaaatg
     7141 cgggtcggtg ccagcagatg atcggctaat ggcttaccat caagttcggt ggtttgcgga
     7201 tcacaaccgc tgacttcaag aattttgcgc accagcgaat agccgttcga gtgcggaccg
     7261 ctggaaccga gtgcaatcag cacatcgccg tcgctgactt tagagccgtc gatgatttct
     7321 gatttttcta ccacgccaac gcagaaaccc gcgacatcgt aatcctcacc gtgatacatc
     7381 cccggcattt ctgccgtttc gccacccacc agtgaacagc ccgattgcag acatccttcc
     7441 gcgataccgc tgatcaccgc tgaagcggta tcaacatcca gttttccggt tgcgtaatag
     7501 tcgaggaaaa acagcggctc tgcaccttgc accaccaggt cattaacgca catggcgacc
     7561 agatcaatac caatggtgtc gtgacgtttt aagtccatcg ccagacgcag cttggtacct
     7621 acgccgtcag taccggaaac cagcacaggt tcacgatatt tttgcggcaa tgcacacagc
     7681 gcaccgaagc cgcccagacc gcccatcact tccggacgac gcgttttctt cactacgcct
     7741 ttgattcttc caaccagagc attacccgcg tcaatatcaa caccggcatc tttgtagcta
     7801 agagaggttt tatcggtcac tgcttgggtc cccacgcgtt acttgcggta gaaaaataaa
     7861 attcggcgca attctaacag ggaaagcaaa cgtttgcgag actgctttac acaacctttt
     7921 tgcacgtctt ttgcccaggc acgcggcgaa agaagacttg tgccagggta aaggttagtt
     7981 ttcggatgga ataatcttct ttcataacca tttgaatata aaataacttt atctcaaacc
     8041 gttatcattt tgactaaagt caacgaaaag aatattgccg ccttgaagaa aggaggtata
     8101 atccgtcgat tttttttgtg gctgcccctc aaaggagaaa gagtatgaag atcgtggaag
     8161 tcaaacaccc actcgtcaaa cacaagctgg gactgatgcg tgagcaagat atcagcacca
     8221 agcgctttcg cgaactcgct tccgaagtgg gtagcctgct gacttacgaa gcgaccgccg
     8281 acctcgaaac ggaaaaagta actatcgaag gctggaacgg cccggtagaa atcgaccaga
     8341 tcaaaggtaa gaaaattacc gttgtgccaa ttctgcgtgc gggtcttggt atgatggacg
     8401 gtgtgctgga aaacgttccg agcgcgcgca tcagcgttgt cggtatgtac cgtaatgaag
     8461 aaacgctgga gccggtaccg tacttccaga aactggtttc taacatcgat gagcgtatgg
     8521 cgctgatcgt tgacccaatg ctggcaaccg gtggttccgt tatcgcgacc atcgacctgc
     8581 tgaaaaaagc gggctgcagc agcatcaaag ttctggtgct ggtcgctgcg ccagaaggta
     8641 tcgctgcgct ggaaaaagcg cacccggacg tcgaactgta taccgcatcg attgatcagg
     8701 gactgaacga gcacggatac attattccgg gcctcggcga tgccggtgac aaaatctttg
     8761 gtacgaaata aagaataaaa ataattaaag ccgactttaa gagtcggctt ttttttgagt
     8821 aaagcgccta taacacataa tacagaggat aatactatga cgcgccgtgc tatcggggtg
     8881 agtgaaagac cgccactttt acagacaatc ccgcttagtt tgcaacattt gttcgccatg
     8941 tttggtgcaa ccgtcctggt gcccgtctta tttcatatta acccggcgac tgtactgtta
     9001 tttaacggta ttggaacgct gctgtatctc ttcatctgta aagggaaaat tccggcttat
     9061 cttggttcca gctttgcctt tatttcaccg gtattgttac tgttgccgtt agggtatgaa
     9121 gtcgcgctgg gcggctttat tatgtgcggc gtgctgttct gcctggtttc ttttatcgtg
     9181 aagaaagcgg ggaccggctg gctggacgtg ctgtttccac ctgcggcaat gggcgcaatc
     9241 gttgccgtca tcggtctgga gctggcgggc gtagctgccg gtatggcggg tttactcccg
     9301 gctgaagggc aaacgccaga ctccaaaacc atcatcatct ctattaccac cctggcggtc
     9361 acggttttag gttccgtgct gtttcgtggt ttcctggcaa ttatcccgat tttaattggc
     9421 gtgctggtgg gctacgcgct ctctttcgca atgggaattg tcgataccac gccgattatt
     9481 aatgctcact ggtttgcgct gccaaccctc tatacgccgc gcttcgagtg gtttgccatt
     9541 ctgactattc tgccagcggc gttagtggtt attgccgaac acgtagggca cctggtagta
     9601 acggctaata tcgtcaaaaa agatctgctg cgcgatccag gtctgcaccg ttcgatgttt
     9661 gctaacggcc tgtcgaccat gatctccggc ttctttggct ctacgccaaa taccacttac
     9721 ggagaaaaca ttggcgtgat ggcgatcacc cgtgtttaca gtacctgggt tatcggtggg
     9781 gcggcgattt tcgctatcct gctttcctgc gtcggtaaac tggctgccgc tatccagatg
     9841 atcccattgc cggtgatggg cggcgtttcg ctgctgcttt atggtgtcat cggtgcttcc
     9901 ggtattcgtg ttttgatcga atcgaaagtg gattacaaca aagcacagaa cctgatcctg
     9961 acttccgtga ttttgatcat cggcgtcagt ggcgcgaagg taaacatcgg cgcggcggag
    10021 ttaaaaggta tggcgctggc gaccatcgtc ggtatcggct taagtttgat tttcaaactg
    10081 atcagtgtgc tgcgccctga agaagtggtg ctggacgcag aagacgcgga tataacagac
    10141 aaataatgta ccgaccgggc agtgttgctg cccggttcaa acatcatggg attctgtggt
    10201 aaacttctcg cgattttgtg aaatcctggt tgaggtatct ctgaacacac cggcacagct
    10261 ctctttgcca ctttatcttc ctgacgacga aacctttgca agtttctggc cgggggataa
    10321 ctcctcttta ctggccgcgc tgcaaaacgt gctgcgtcag gaacatagcg gttacatcta
    10381 tctctgggca cgcgaaggcg cggggcgcag ccatctgctg cacgcggctt gcgcggaatt
    10441 gtcgcagcgt ggcgatgcgg tgggctatgt cccgctggat aaacgcacct ggtttgttcc
    10501 ggaagtgctc gacggtatgg agcatttgtc gctggtctgt atcgacaaca ttgagtgtat
    10561 tgcaggcgat gagttgtggg agatggcgat tttcgatctc tacaatcgaa ttctggaatc
    10621 gggcaaaaca cggttgttga tcaccggcga tcgtccaccg cggcagttga atctgggatt
    10681 accggatctc gcgtcgcgac tcgactgggg gcagatctac aaattgcagc cactttctga
    10741 tgaagataag ttgcaggcgc tacagttacg cgcgcgtttg cgtggttttg aactgccgga
    10801 agatgtgggg cgtttcttgc tgaagcggct cgacagagaa atgcgcacgc tatttatgac
    10861 gttggatcag ttggatcgtg cgtcgattac cgcgcaacgt aagctgacca ttccgtttgt
    10921 gaaagaaatt ctgaagttgt agataaggtg tttattgtcg gatgcggcgc gaacgcctta
    10981 tccgaactac gcgaagttaa tccatagtat tcgtaggccg gataagacgt ggcaagcgtc
    11041 gcatccggca atgtgcttca actagcgatg gagcacatct taacccacga tttccagtac
    11101 ctgctccggt ggacgaccaa tccgcgcttt gccattcgcg accacaatcg ggcgctccat
    11161 cagcttcgga ttatccacca ttgcctgaat cagcgcctct tcgctaagcg aactgtcagc
    11221 caggttcagt tctttataaa gatcctcttt ctggcgcatc aattcacggg cgctattcat
    11281 tcccagcatt ttcagcaaat cgcgcagcgt tgccgcatca gcgggtgtct caaggtagag
    11341 caccacttct gactccacgc cgttttcttt cagcaggttc agcgtttccc ggctctttga
    11401 acagcgtggg ttatggtaga tttttacctg tttggtcatt gttcttcctt taatgcgaat
    11461 tacatcttgg tataaggctt aaagcgttcc tgcagctggc gcaactggtc gatgcgcgca
    11521 tcgtaacgcg cttgttgcag gctgcctaat ttcacctgcg aactggcgct actcaacagc
    11581 gaaatggcct gatcgagtcg tccggcgagc gcataacctt ctgctcgcgc agccagctcc
    11641 tgatcacggt tatttagcgc ggcttccgcc tgtgccagca aatcccagcc gttgctgtca
    11701 tctttattat taaaggtgta gcgattcaga atattggccg cttcttgtgg ttgaccgcct
    11761 tgcagataag cgttcgccag gttgagctgc aacaccggat tggtgcgtaa atcgcgggca
    11821 tttttcagac gattgatcgc ctcattggct ttgttttgcc caagatcgat atcagtagcc
    11881 agatcgagat accatgcgtt gccaggttct gccgccagta acggttgcag cgttttacgc
    11941 gcctcgtcgt atttattggc ttccatcgcc tgtaaagcac gaccatattg cgccgctcgt
    12001 tgctgacgaa cgtttccttt cgcccattca tccagcaaat cactggtgag ctggttacgt
    12061 ccggaattat acatccccag tgtgcgcgct ttcgccagat agaaatcttc cgacgactgc
    12121 accaccatcg ggcgcatctg attagcacgg ttgcgggcat ctgccagacg actttccggc
    12181 aacgggtgag tcaataaaat ttccggcgga cgcgaggagt aacgcgcctg atcgagtaat
    12241 ttttcgagga aggttggcat cgcctgcgga tcgaatcccg agcgttgcag cacctgaata
    12301 ccaatgcggt ccgcttcctg ttcattttgc tgggtgaaac tgatcatccc ctgacgcgtt
    12361 cccgccagtg taccggtcag cgccgccatc cccgcctgcg gactggccat cgccagtaaa
    12421 atagaaccta acgcgccgac ccaggtcagc ggcgcgctgc gctgctgatc ttccatcgct
    12481 cgcgccaggt gacgttgggt gacgtgggag atttcgtgcg ccataactga agccagttga
    12541 ctttcgttat cggaataacg gaacagggca gagtgcagca ccacgttgcc gccaaagaaa
    12601 gcaaaggcgt taatttcgtc gttgttgatc agaaagaaat gaaacggtgt cttaaccgaa
    12661 ttggcatgcg aaaccagacg catccccagc gaattaatat attgcgttaa cagcgggtca
    12721 ttaattaacg gcgcgctgcc gcgtagctgg cggacataat agtcgcccat ctgcatttcc
    12781 tgaccaatgg aaagcgtgct tcctgcggag gttcccatat ccggcaaggt gtctgcgctg
    12841 tcggcaaacg ccggggctac ctgaccaatg gtcatagcag caatgagggt tgcaaccagg
    12901 ttttttttca actgcctgaa cataacctct atcctgtatt tctgaagata gtcatttgac
    12961 cgatactgtt aaatatcgtt cccaccgcgc cgcctttgag tgtaacggcg aagattagcc
    13021 cactcaaatg acttttcagt ttcctgaatc cgtcataaaa agcgaagtct tttttgtgac
    13081 atttcgatac aattgcggat cgcaatcccg ctcttgaggt tcagggaagg tctttatgct
    13141 cgaaatgttg atgcaatggt atcgccgccg ttttagcgac ccggaagcga ttgccttgct
    13201 ggttatttta gttgccggat ttggcattat ctttttcttt agtggcctgc ttgctccgtt
    13261 gctggtggct attgtgctgg cctatttgct ggaatggcca actgtgcgcc tgcaatctat
    13321 tggctgctcc cgccgctggg cgacgtcgat tgtattggtg gtttttgtcg gtatattgct
    13381 actgatggcg ttcgtggtac tgcctatcgc ctggcaacag ggcatctact taattcgcga
    13441 tatgccgggg atgctcaata agctttctga ctttgccgcc acgttgccgc gccgctatcc
    13501 ggcgttaatg gacgcgggca ttattgatgc aatggccgaa aatatgcgca gtcggatgct
    13561 gaccatgggc gattcggtgg tgaaaatttc cctcgcctcg ctggtcggtt tgctgaccat
    13621 tgccgtctat ctggtgctgg tgccattgat ggtcttcttc ctgctgaaag acaaagagca
    13681 gatgctgaac gccgttcgtc gggtgctgcc gcgcaaccgt ggactggcag gacaggtgtg
    13741 gaaggagatg aatcaacaaa tcaccaacta tatccgcggc aaagtgctgg agatgatcgt
    13801 ggtggggatc gccacctggc tggggttctt gctctttggg ctgaactatt cgctgctgct
    13861 ggcggtgctg gtcggcttct cggttcttat tccgtacatt ggcgcatttg tggtgaccat
    13921 tccggtggtt ggcgtggcgc tattccagtt tggcgcaggc acggaattct ggagctgctt
    13981 cgcggtgtat ctgattattc aggcgctgga cggcaacctg ctagtaccgg tgttgttctc
    14041 cgaagcggtt aacctgcatc cgctggtgat tattttatcg gtggtgatct tcggtggttt
    14101 gtggggattc tggggcgtat tcttcgccat tccattggcg acgctgatca aagccgtgat
    14161 ccacgcctgg cccgatgggc aaatcgcgca agaataatct tcagcccgac gaactgtttc
    14221 gccgggctga tttttcagga ttcctgacgt aaataaatag cccgataaca catactcacc
    14281 agcactgcac cgccgataat attacccagc atcaccggga gcagattggc ggtaataaaa
    14341 tggctgaccg tcagtgccgg aaaattgtct gcgctactgt gcgccagttg ccagaaggag
    14401 gtaggggcga aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag
    14461 tgctcaaagc cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg
    14521 cataacgaac gcccgcaata actcatccac agcgccaggc aaaccatcag attgcacata
    14581 atgccgaggc tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa
    14641 accgcgactc cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa
    14701 ctgaaacagg caatacctgc cagattaccg caggccacta gaagtgcgtt aatcagccaa
    14761 gttcgccaac taataacgcc ccgacttttt gccattaccg tcattaccga cgaggtgaac
    14821 aggttggtgc cgcaaaccgc cagcaaaata aagccaagtg taaagcaaag gccgcccacc
    14881 agatgggtta atgcctgtga agacggggca tcggcgataa ctgaaaggta aaaagtaaat
    14941 ccgatcgcca taaacacgcc agcggacatc gccattaaaa agaccgacac tttactgcgg
    15001 gcaattttat gggcagcaat ccgttcagcg attgccgctt ttctggcgct caactgcaag
    15061 tcgaaagaga gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga
    15121 accgagatcc ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt
    15181 gtcgccgcgc cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg
    15241 cgctgacgtt cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa
    15301 ctgcgaagcg ctgaatcttc atttagcgtc ggcagtaaac ggctttgtcg gacatttaga
    15361 tgtaaattca gattgttacc acgagtcaac agtaccgccc gctcaatcac gttttccagc
    15421 tcgcgcacgt tgcccggcca atcccacgac atcaactggc gtagcgcctc ggtcgggatt
    15481 gcgtcaattg cgcgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga
    15541 gggatatctt ccggacgatc gcgtagcggc ggcaattcca gtgggaagac attcaggcga
    15601 taaaagagat cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg
    15661 gcggcaatga ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc
    15721 cgctcctgca atacgcgcag caatttaggc tgaagttcta acggcagatc gccaatttca
    15781 tcgagaaaca acgtaccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca
    15841 ccagtaaacg cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg
    15901 gcagcgcagt tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct
    15961 ctggcgatca cctctttgcc agttccggtt tcaccgcaaa tcaacaccgt actgtcgctc
    16021 ttcgccacaa tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg
    16081 tcaccgacgc ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa
    16141 ctttcctgca aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg
    16201 cgatcggcta tgtgttgtag tagctggcag ttttcctcac taaagagagt ggaagaggta
    16261 tgcgccagta acaatgcacc cggtgtatgg ttgccaaagg taagcggtat caggagcgca
    16321 gattcgcagc cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga
    16381 tcgtctgcac ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca
    16441 ctgtcgccag gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac
    16501 cacaggctga acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga
    16561 ccgaaaaaat gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt
    16621 gacagcactg cattagtgat atccaccagt acccgatgat gatcgcgatc tttctccagc
    16681 aaacgcagcg ccacctgttg ttcttcatca ttgagccatg attgcagaac cggggtgata
    16741 atgcgtgcca gccagttaaa cagtgtgcaa tcctggggag taaaacggct gccgtgggta
    16801 cgaataaagc gtagctccgc cattccacta ctgccgagaa ggacggcgta acgcgaataa
    16861 tcgaccatct cgccctgata ctctactgtt gccgtcacat cgctgccatc atcatggtag
    16921 cggtaataga agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc
    16981 ggcggcagcg ggatgaaggc gcgtaataaa gacgccttgc cgtgtttctg tgctaaccgc
    17041 tccgcgagca ttccgtttac cgcttcaatt gttattcctt gtggcggggc aaacatcgcc
    17101 tcgtctgaca tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc
    17161 tggccatcag atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa
    17221 tgctacgcgc ctgccacaca gacgtcgcgt catctgccag tagcgcgaat gcccgggtca
    17281 acagggcggt gtggctggcg tcaataacaa tctcaccaaa ggtcatcacc cccagcatct
    17341 tacgtcgggc ttgctcctct tcgacctgtg caagccaatc ttcgtattcc gtcagtgggc
    17401 agcggaaggc gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt
    17461 aatacattac ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc
    17521 gcagcgtcca gaaaacaatt tccccgcact cctcagccat gtaacccccg gagaacgcga
    17581 aagagttgct gaacgatact attgagacgc ggatcgtcgg cttcgcgcaa ccaggtgtta
    17641 acccgatttc ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc
    17701 ccctgacgat agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc
    17761 gggatctgtg gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccacagcg
    17821 tgaatcttct gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcagccggt
    17881 gttggcgggc agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag
    17941 acgctgtaga gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacag
    18001 attttatgat cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg
    18061 actgcgccag taaataacaa aatatcggcg tgacgcggtg atgaaacaac cttaatgcca
    18121 aaacgttctg cgtcgaatac tggtgtgatg gcagcaaaaa tttcgatttc acaggcgttg
    18181 cagccgccgc aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc
    18241 tgcttcatct tttgcgtttg ctcgtccagc gtgatgggct ggctgacata ttgcgtaagc
    18301 actggactca tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgatcagcg
    18361 tcgcgcgttg tttgcactcc gggcagacgc tcgcttgcgc ccgcaacatt tcgcggtttt
    18421 gtggcgcatt ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg
    18481 caaacgggcg ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat
    18541 cggctttatt ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc
    18601 acacttcttc acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg
    18661 aattttgctg gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc
    18721 aggcaataca ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca
    18781 gtggcgcgaa gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact
    18841 tcagcatctt ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga
    18901 tctctttata cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt
    18961 cggtacagga gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat
    19021 tgccgcgcag catgtaacgc aacaccggcc agttggcgta ggtggcggca cgacagcgcc
    19081 agcggaacaa tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg
    19141 cttcaacaaa gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagcg
    19201 gggtatccgg catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt
    19261 tgacacggac catcacgcgg gagaaaacat cgccgccggt gaaggtaaac agtgtttttg
    19321 gaatattgcc gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc
    19381 cgcggatcag cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa
    19441 tgccctgagt gcgttgttcc atattcggca tagcaagcag catctctacc agctccgaca
    19501 cgtcggcgcg catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat
    19561 cgcggcgaac accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt
    19621 ccgccatcgt catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaaac
    19681 caacaaaatg gcaggagagg ccaaggttga gcaaatgact gtgtagccgt tcgacttcca
    19741 gcagaatcga gcgaatggta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa
    19801 ccgagttggt ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cggtccgata
    19861 agaaggtcac ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga
    19921 cataaaacag gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt
    19981 gacccggttc atcggaggtg atatgcagcg ggccgacagg gatcacccgc gcatcgctgt
    20041 tgccctcatt gataaacgca tacgtttcgg aatctgtcgt cggttcaggg cgcagtcgat
    20101 aatccatcgc atctttgcgc agcggatgca tatcttccgg ccagtcatcg ggcaacacca
    20161 ggcgacgctg atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct
    20221 cgccccagac cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat
    20281 cgaccagtgc cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat
    20341 aaaccgcgta atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc
    20401 catcatgttg ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa
    20461 tggtgacctg ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt
    20521 tcagggcggc gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc
    20581 tccgaaacag atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc
    20641 aggtcgtgcg tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt
    20701 tgtggaatat gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg
    20761 gtcaaccagc cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc
    20821 gcagccatcc gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata
    20881 atcagcaggt gattacgtgc cagtcctgcg gtaacggtca taaattcgct aagaaaaatg
    20941 ttgaagggcg gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat
    21001 ggcatgattt tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt
    21061 acattgccgg aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa ggtgtgcagc
    21121 agcgcggcaa aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg
    21181 ttctccacgc tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat
    21241 gccgccacgg caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg
    21301 ccgatggcct ggcagataat gatgtaatag cgaattagca ccaacagcgc gcagttcagc
    21361 aatacggcgg agagcagggc gctgaccgga ctcggtgctt cactgtgagc atccggcagc
    21421 caggcgtgca tgggaaatag cccggttttg gtgccaaagc caattagcac aaacacaaag
    21481 gccaacagca ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat
    21541 atcgccattt ctgcctgcgg cataacgctg gcgacgttgg cgtataccag cacggtaccg
    21601 aacagaccaa aagcgacacc aacagtacaa ataatgatgt acttccatgc tgcttccagc
    21661 gatgaacgct gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc
    21721 gccgcccaca tcacaatcag gttattgctg gtaacaacca gcagcatggt aaacaaaaac
    21781 agatggaaga aaccgtagta atcgcacagc gtaacggggg aaagctcgcc gtgtgccact
    21841 tcatgacgca tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg
    21901 gcgaggaaca aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct
    21961 cctgcatcag cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct
    22021 aagctatgta atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa
    22081 cagagcagcg aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc
    22141 atctcattat cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca
    22201 tatttttctt gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc
    22261 tatttccacc agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt
    22321 ttccatcagg cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag
    22381 caggccaagc agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat
    22441 cggtagcttc acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag
    22501 agccatcatt gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc
    22561 agcgtaagtc atgattaacg gtaccagcag cactttggtg ataaaggcac tggcggacca
    22621 gatcagcagt tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac
    22681 cagcgactgg caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa
    22741 cagcgatgtc agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca
    22801 tcatctcctt actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc
    22861 agtacaatca acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg
    22921 ctcggtttgc caggcacaac gcgaccaaac cagcgaataa accaggcgaa actggcgacc
    22981 gattcaatca tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca
    23041 aaaccggcag caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacgccggta
    23101 atcgccagcg ctgccacgca gaaacccacg cctggcaacg gcagggtgtg caatacgcca
    23161 cgcagacgcg gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa
    23221 aacaggcttt tagcgaacgc gtggttgacg atgtaggcga tgctgccctc cagtgccagc
    23281 cgcccacttt caccatcgat gcggcgtgga gataggcgct gatcggtgtt ggcgcttcca
    23341 ttgcgtccgg tagccacgct tgcatcggta gctgggccga tttcccccac gcggcaaaca
    23401 ggatgccgcc ataaaccaga taacgtgcgt cgccatgtaa ctcgctcatc gcgctaagca
    23461 caaacgttcc ggtctgcaaa aacagcgtgg cggcggcaag atacaacccc aacgaaccga
    23521 tatgggtgat aagcaacgct tttagtgctg aacgctgcgc tttatcgctc tggtaataac
    23581 tgatcaacgc ccaggagcag ccgcccgtaa tttcaaaaaa caacaactga ccgagcagcg
    23641 tcgaggagag taccagtccc gccatcgcgc cgataaacaa cagcaaaaat gcgtaataac
    23701 gattcgtgcc gttatgcggg tgttcgcgat ttttatccgt cagataaccc gtggagtaga
    23761 tcgtgaccag caaaccgaga aacaccacca caaacagaat cagcgtactc acgcgatcaa
    23821 tgaccaggcc aaacaatgcc acatccccca cgttgaccaa cgtcaacgtg acggcaactt
    23881 tatcggcctg ataaaacgcg gagatcagcg acaacatgca cagcgtggtc agtgcggcga
    23941 acaaaacccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat
    24001 aaaaggcagc attaacgtcg tcagagcaag attttccata ttccgtcagt gctccttaca
    24061 gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc caggtcacat
    24121 ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa
    24181 aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag gcgagagaaa
    24241 gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc atcacgacct
    24301 gtttcagccc cagcccccat ttcgctagcg ccagcccggc accggaatat tcggtcagcg
    24361 ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt cccatctcaa
    24421 tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc gagttccagc
    24481 ccgttgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc agcgccaata
    24541 ccagcagtga gagaatgagc atgggttcga ccagaatgcc gagcgtcaac tcgcgactgg
    24601 caccgactcc cgcaaacggg cttccggtat ccagcccgga aagagcaaag aaaaaacgaa
    24661 acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg
    24721 taataaaaag tggtagggcc atcgccagca ccagcatgct gctgattaat acccacggca
    24781 tcaggcggaa catcagacct gaagatatag gcgcaacttc ctggcgttta aacagtttgt
    24841 ggatatcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata cgcgcgcgta
    24901 tctgccggga aatacccgta aaaagcgggg ttagcatcag cagaatcacg gcctgtgcta
    24961 acgcaaaaat gaccagatat ccgtcgtaaa gagtttgtct catggtgatt tccttagacg
    25021 gcaatagcga ttagcagcac aaccagtgcg gcgaccacat acaggcaata gagacgaaag
    25081 tcgccgcttt gcagatgctg tatttctttg gccagccgtt gggtggcgct cacgatgggg
    25141 cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt gacgtgcgca
    25201 aaacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa taacgcagag
    25261 aagaccacac gcagcggctg catcacgcca ttgcctgacg gggccatcgc attttcccag
    25321 ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac
    25381 cacagccagt aaaggccagg cagcaaaggc atcgccagta gtaacagcaa cagtaatgag
    25441 ggggtgacct gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc gacagtgacg
    25501 ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag ccaactggca
    25561 ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat catcgtattt
    25621 ggcacttcct gtgcatcttc agcgtgtgta ctgcgcggcg caccacagaa agtaataccg
    25681 tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat caccatagca
    25741 ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa cagcgactgc
    25801 caggtgtacc attcgctgat aaaaccattc agcggaggaa gggctgatat cgcgaggcaa
    25861 ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc cattttttcc
    25921 atgtcgtggg tatgcaaacg cgaaataatc gcgcccgcgc cgagaaatag cagccctttg
    25981 aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac ggtgagcagc
    26041 gggtcgtgca gtgacagacc gaccatcgcc acaccgactg ccagcaaaat aatgccgacg
    26101 ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag cgcatacagc
    26161 acgcccagga gcgcggagat tgcgccgatc gccatcacca gagtgcccca ccacagcggc
    26221 aaacccgttt gcgccagcag gtccatcgct actttcagga tgccgaaaat accgattttg
    26281 accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg agcgcgcggc
    26341 aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa ggccagcagg
    26401 aacaccgccg acgccagccc cggagaaagt gaaagcgtgc ggaaactggc aaaatcgagg
    26461 ctgccgcttt cgcgccccat cagcaagaag gcgatcataa tcagcaccga tccggcgtgg
    26521 gcgataaaaa agtagagcat gccagcgttg atcgacgttt tatcctgcct ggcaatgacc
    26581 agaaaccagg aagacagcga catcatttca aacagcacga tgaaccaaaa agcgttgtcc
    26641 atcaccagca gggcaaccat cgatgcgatg aaaagattca taaagaagcc catcgccgcc
    26701 gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat agagcgaaca aaccaccacc
    26761 agcaaggaga taaccagcac cataaatgcc gagaggctat ccattcgcag ggttaaatcg
    26821 gcaaagtcaa aaggggtggc aaaatgggcg actaaagtta caccagcatg taattgcgta
    26881 attccgctga tgatgccaat cacgccgccc accagcgatg tgatgccgga aagcttaata
    26941 gccagtctgt ccagaccgag taaaaacagc gaagccagac tggcaaacag atagaggatc
    27001 agcgaccagg ttaataattg cagggcatcc attagtgttg ctcctgagtg agggaaaggg
    27061 gatcctcccc gccattggca aaccagcttg cggcaaggcg ctgtttttct ttcatctgac
    27121 gttgcaggct atcgtcggtg atcagccgta acgcctgatt cgggcaagcg cgaacgcagg
    27181 ccggaccttc tggcaagaaa tcacacaggt cgcatttcac cgcgacggtc tggacacccg
    27241 gttcccagcg cagcaacgca tgttggggca gcacgttttc ttcgccgtct ttgagtgagc
    27301 cttcagcctg aaaaacatat tgcgcatggg cattcaccgg acggcttcct gaagcgctga
    27361 ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc aatacagagg ctttcgttga
    27421 gttggatcgc atcatccctc tgagagatgg cattgaccgg gcagacctgc aggcaagggg
    27481 cttcctcaca gtgatgacac acgacagggg cagtgattgt tgacgtcttc gccagggcca
    27541 ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc cgaacaggca gcgagacagg
    27601 tattacatcc tgtacaccac agtggttcgg ccaccacaaa gcggttcatg tcggctcctt
    27661 gaaaatgata tgaaacattg catttcatat tgcataaagg atgccattct gattaactca
    27721 atatattcaa taagttatgt attgactgga ttctgaacgg tacaaaaatg atggtcatta
    27781 acgttcagca aaaaatggat tcgacacccg tgtcgcaggg gaaagcgaga atgtgatctt
    27841 gatcacgaga agtgaggtat gctggccgca agcgcagcca gcacggaatg gagcaaagta
    27901 atcaggcgtg ttctttcagc cagttcagca caacgtcgtg gtgattgctg gttttgaaat
    27961 cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag gaagctgatg cgatgaatgc
    28021 catcgtaggt tttgcccatg aaggactttt caccccagac gccgaattgt tcgcacacct
    28081 ggtggtcctc atcagacagg agcgtaaagt taagcagctc tttttccgca aaacgggaga
    28141 gtttttcggg tttatcggtg ctgataccca gcacatcaac gcccgctttt ttcaactcat
    28201 ccatgttatc gcgtaagccg caggcctgta cggtacagcc gggggtcatg gctttcgggt
    28261 agaaataaac cagaacacgc tgtccctgga agtcggtcaa attaacttgt tctccgtctt
    28321 gatccggcaa gctaaatttc ggtgcgatat caccggcttt cagtggattc attacttaac
    28381 tccatcctgt tcatcatgtt gggaataatt gacgacgtta atactgcctt gtgcattgag
    28441 ttctgtacat agggctttga acgcttgctc aatatttgct gcgtccgcag atgcggggct
    28501 gtgggcggtt atctgaatat gcaactgcgc agcccgttca ttttcagcag gttgcgtgcg
    28561 cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat
    28621 taaatgcggg gagtctgcca catcgacctg aacccagaca gatgctggca ttggcggacg
    28681 cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc agttcggcac ctttcaacgg
    28741 taacgttgat tcaatcagag taatggcatt ccatgaaccg gaaagcagca taataaacgt
    28801 gaactcttct cccagcatcg ccaggcgact gtcttcaata ttacagccgc aactactgac
    28861 atgacgggtg atggtgttca caattccagg gcgatcggca cccaacgcag tgatcaccag
    28921 ataatgttgc gatgacagtg tcaaactggt tattccttta aggggtgagt tgttcttaag
    28981 gaaagcataa aaaaaacatg catacaacaa tcagaacggt tctgtctgct tgcttttaat
    29041 gccataccaa acgtaccatt gagacacttg tttgcacaga ggatggccca tgttcacggg
    29101 aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt aatgtctgtc gggctagctt
    29161 gaaaaaactg attgattatc atgtcgccag cggtacttcg gcgatcgttt ctgttggcac
    29221 cactggcgag tccgctacct taaatcatga cgaacatgct gatgtggtga tgatgacgct
    29281 ggagctggct gatgggcgca ttccggtaat tgccgggacc ggcgctaacg ctactgcgga
    29341 agccattagc ctgacgcagc gcttcaatga cagtggtatc gtcggctgcc tgacggtaac
    29401 cccttactac aatcgtccgt cgcaagaagg tttgtatcag catttcaaag ccatcgctga
    29461 gcatactgac ctgccgcaaa ttctgtataa tgtgccgtcc cgtactggct gcgatctgct
    29521 cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt atcggaatca aagaggcaac
    29581 agggaactta acgcgtgtaa accagatcaa agagctggtt tcagatgatt ttgttctgct
    29641 gagcggcgat gatgcgagcg cgctggactt catgcaattg ggcggtcatg gggttatttc
    29701 cgttacggct aacgtcgcag cgcgtgatat ggcccagatg tgcaaactgg cagcagaagg
    29761 gcattttgcc gaggcacgcg ttattaatca gcgtctgatg ccattacaca acaaactatt
    29821 tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac
    29881 cgatacgctg cgcctgccaa tgacaccaat caccgacagt ggtcgtgaga cggtcagagc
    29941 ggcgcttaag catgccggtt tgctgtaaag tttagggaga tttgatggct tactctgttc
    30001 aaaagtcgcg cctggcaaag gttgcgggtg tttcgcttgt tttattactc gctgcctgta
    30061 gttctgactc acgctataag cgtcaggtca gtggtgatga agcctacctg gaagcggcac
    30121 cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc ggtgacctcc ggtgattatg
    30181 caatcccggt gaccaacggt agtggtgctg tcggtaaggc gctggacatt cgtccaccag
    30241 cccagccgct ggcactggtt tctggcgcgc gtacccagtt cacgggcgat accgcttcat
    30301 tgctggtgga aaatggtcgt ggcaatactc tgtggccgca ggtggttagc gtgctgcagg
    30361 cgaaaaacta caccatcacc caacgtgatg atgctggtca gacactgacc accgattggg
    30421 tacaatggaa ccgtctggac gaagacgagc agtatcgtgg tcgttatcaa atctctgtta
    30481 agccgcaggg ttatcagcag gcggttacgg ttaaactgct gaacctggaa caggcgggca
    30541 aaccggttgc agacgcggct tccatgcagc gttacagcac ggagatgatg aacgttattt
    30601 ccgccggtct ggataaatct gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca
    30661 ccactatgga cgtacaaagt gcagctgatg acaccggttt accgatgctg gtcgtacgcg
    30721 ggccgttcaa tgtggtttgg caacgtctgc cagcggcgct ggaaaaagtg ggcatgaaag
    30781 tgaccgacag cacccgttcg cagggtaaca tggccgtaac ttataagccg ctgtctgaca
    30841 gcgactggca ggaactgggc gcgagcgatc caggcctggc atccggtgac tataaactgc
    30901 aggttggcga tttagataac cgcagcagcc tacagttcat cgatccgaaa ggtcatactc
    30961 tgactcagag tcagaacgac gcgctggtag ctgtcttcca ggctgcgttt agcaagtaaa
    31021 aatacagggc tggaatcatc cggccctttt ttctgatatg atacgcaaac gtgtgcgtcg
    31081 gcaggaaaac gcgattttag cggtaattcg cacgaaattt gtttgtcgga cgtagttcgg
    31141 ataaggcgtt cacgccgcat ccgacaaaac atccggcaca ccagacagca aaagatttta
    31201 aaacgttaat tcacacccag gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg
    31261 taaagcgaaa accgtataca gcacggaaaa cccggacctg ttggtgctcg aattccgcaa
    31321 tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa
    31381 caacaagttc aactacttca tcatgagcaa actggctgaa gcgggtatcc cgactcaaat
    31441 ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag ctggatatgg tgccggttga
    31501 gtgtgtcgtg cgtaaccgtg ctgctggctc tctggtgaaa cgtcttggaa tcgaagaagg
    31561 tattgagctg aacccgccgc tgttcgatct gttcctgaaa aacgacgcca tgcacgatcc
    31621 gatggtcaac gaatcttact gcgaaacctt tggctgggtg agcaaagaga acctggcgcg
    31681 tatgaaagag ctgacctaca aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg
    31741 tctgattctg gtcgacttca agctggagtt tggcctgtac aaaggtgaag tggtattggg
    31801 cgatgagttc tccccggacg gcagccgcct gtgggacaaa gaaacactgg agaaaatgga
    31861 caaagaccgt ttccgccaga gcctcggtgg cctgatcgaa gcctatgaag ccgtcgcccg
    31921 ccgcctgggt gtacagctgg actgattttt ctggttgctt gtttctttag ccggagggta
    31981 ctccggctaa atgtctttga tacccccgct acaatacttc tcattatctc cctttgacag
    32041 gaaagtcata ccaggccaat atgtatatac ataacgtata tcccaaaaag ggggggctat
    32101 ggaaactact gtatttttaa gtaatcgcag tcaggccgtc agattaccga aagcggtcgc
    32161 tctgccagaa gacgttaaga aagtagaaat tattgcgatt ggtcgtacaa gaatcatcac
    32221 tcccgccggt gagtcctggg acagttggtt tgatggcgaa aatgtgagtg cggattttat
    32281 ggatatccgt gatcaacccg caatgcagga aagggaatca ttttgatgct gaaattcatg
    32341 ctggatacca atatctgtat tttcactatt aagaataaac cagtacatgt cagagagcgg
    32401 tttaacctga attctggccg gatgtgtata agttctgtaa cattgatgga gcttatctat
    32461 ggggcagaaa aaagtcagat gccagaacgc aatttggcgg ttattgaagg tttcgtatct
    32521 cgtttggtag tgcttgatta tgatagtgct gctgcgacgc ataccgggca aatcagggcc
    32581 gagcttgccc ggcagggaag gcctgttgga ccttttgacc agatgatagc gggacatgct
    32641 cgcagtcgcg gtttgattgt ggttaccaac aatacccgag agtttgagcg agtggcggga
    32701 atcagactag aagactggag ctaacattct cttttacttc taacattcgt aaattatata
    32761 acgtggcatg aagccttaat tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc
    32821 tctgaaccac ctacgatcat ttctataatg aatatattgt tgaggtatct atgcgttggc
    32881 aagggcgacg tgaaagtgac aatgttgaag acaggcgcaa cagctccggt ggtccgtcta
    32941 tgggcggtcc tggttttcgc ctgccaagcg gcaaaggcgg gctgatttta ctgttagtcg
    33001 tgctggttgc aggctactat ggtgttgatt taaccgggtt aatgactgga cagccggttt
    33061 cccaacaaca atcaacgcgg tccattagcc ctaatgaaga cgaagctgca aaattcacct
    33121 cggtgattct ggcaaccacg gaagacacct ggggacaaca gttcgagaag atgggtaaga
    33181 cctatcagca accgaagctg gtcatgtacc gtggaatgac gcgcaccggc tgcggggctg
    33241 gtcagtccat tatggggccg ttctattgcc cggcggatgg cacagtttat atcgatctct
    33301 ccttctatga tgacatgaaa gacaaacttg gcgcggatgg cgattttgcc caggggtacg
    33361 ttatcgccca tgaagtcggt catcatgtgc agaaactgtt aggcatcgag ccgaaagttc
    33421 gtcaactgca acaaaacgcg acgcaggcgg aagtgaaccg cttatctgtg cgtatggaac
    33481 tccaggccga ctgttttgcc ggtgtctggg ggcatagtat gcagcagcaa ggcgttctgg
    33541 aaaccggcga tctggaagag gcgctgaacg cggcgcaggc catcggcgat gaccgtttac
    33601 aacagcaaag tcaggggcga gtagtaccag atagtttcac acatggcact tctcagcaac
    33661 gctacagctg gtttaaacgt ggtttcgaca gcggcgatcc ggcacaatgc aatacttttg
    33721 gtaaaagcat ttaacttccg gggcagggat ggctgaactg actgcgcttc acacattaac
    33781 agcgcaaatg aaacgtgaag ggatccgccg cttgctggtg ttgagcgggg aagagggttg
    33841 gtgttttgat catgtgctta agttgcgtga tgccttacct ggcgactggc tgtggatttc
    33901 gccgcagcca gatgctgaaa accactgttc accctcggcg ctacaaactt tacttgggcg
    33961 cgagttccgg catgcggtat tcgacgcccg ccacggcttt gatgccgctg cctttgcagc
    34021 acttagcgga acgttgaaag cgggaagctg gctggttttg ttactccctg tatgggaaga
    34081 gtgggaaaac caacctgatg ccgactcgct gcgctggagt gattgccctg accctattgc
    34141 gacgccgcat tttgtccagc atctcaaacg cgtacttacg gcggataacg acgctatcct
    34201 ctggcggcaa aaccagccgt tctcgttggc gcattttact ccccgtactg actggcaccc
    34261 cgcgaccggc gcaccacaac cagaacaaca gcaactctta cagcagctac tgaccatgcc
    34321 tccgggcgtg gcagcggtaa cggctgcgcg tgggcgcggt aagtcggcgt tggcagggca
    34381 actcatttct cgtattgcgg gcagtgcgat tgtcaccgcg cccgcaaaag cggcaacgga
    34441 tgtactggca caatttgcgg gcgagaagtt tcgctttatt gcaccggatg ccttgttagc
    34501 cagcgatgag caagccgact ggctggtggt cgacgaagcc gcagccatac ctgcgccgtt
    34561 gttgtatcaa ctggtatcgc gttttcctcg aacgttgtta accactacgg tgcagggcta
    34621 tgaaggcacc ggacgtggtt ttttgctgaa attttgcgct cgctttccgc atttacaccg
    34681 ttttgaactg caacagccga tccgctgggc acagggatgc ccgctggaaa aaatggttag
    34741 tgaggcactg gtttttgacg atgaaaactt cacccataca ccacaaggca atatcgtcat
    34801 ttccgcattt gaacaaacgt tatggcgaag cgagccagaa acgccgttaa aggtttatca
    34861 gttattgtct ggtgcgcact accggacttc gccgctggat ttacgccgga tgatggatgc
    34921 accagggcaa cattttttac aggcggctgg cgaaaacgag attgccggag cgctgtggct
    34981 ggtggatgag gggggattat ctcaacaact cagtcaggcg gtatgggcag gttttcgtcg
    35041 cccgcggggt aatctggtgg cccagtcgct ggcggcgcac ggtagcaatc cactggcagc
    35101 gacattgcgt ggacggcggg tcagccggat agcagttcat ccggcgcgtc agcgggaagg
    35161 cacagggcag caacttattg ctggtgcttt gcaatatacg cgtgacctcg actatctttc
    35221 ggtgagtttt ggttacaccg gggagttatg gcgtttctgg caccgctgcg gttttgtgct
    35281 ggtgcggatg ggtaatcatc gtgaagccag cagcggttgc tatacggcga tggcgctgtt
    35341 accgatgagt gatgcgggta aacagctggc tgaacgtgag cattaccgtt tacgtcgcga
    35401 tgcgcaagct ctcgcgcagt ggaatggcga aacacttcct gttgatccac taaacgatat
    35461 cgtgctttct gacgacgact ggcttgaact ggccggtttt gcttttgctc atcgtccgct
    35521 attaacgtcg ttaggttgct tattgcgtct gctacaaacc agtgaactgg cattaccggc
    35581 gctgcgtggg cgtttacaga aaaatgccag tgatgcgcag ttatgtacca cacttaaact
    35641 ttcaggccgc aagatgttac tggtccgtca gcgggaagag gccgcgcagg cgctgtgcgc
    35701 acttaatgat gttcgcactg agcgtctgcg cgatcgcata acgcaatggc aattttttca
    35761 ctgactcctt caattttctc ccatggtcat tgtgagtcag cggcgtaaaa ttaatgacat
    35821 cagttaagga gttcgccatg aaacatgacc attttgttgt tcaaagcccg gataaacctg
    35881 cgcaacagtt gttactgctt ttccacggtg tcggggataa cccggtggca atgggggaaa
    35941 taggtagctg gtttgcaccg ctgtttcctg atgcgctggt ggtgagtgtc ggtggcgcgg
    36001 agccgagcgg taatccggcg gggcgtcagt ggttttcggt gcagggtatt acggaagata
    36061 atcgccaggc gcgtgtggat gcgatcatgc cgacgtttat tgaaacggtg cgctactggc
    36121 agaaacagag cggagtaggg gctaacgcca cggcactcat cggtttctct caaggcgcga
    36181 ttatggtgct ggagagcatt aaagccgaac cgggccttgc ttcacgcgtc attgctttta
    36241 acggacgcta tgcgagcctg ccggaaactg cgtctacagc cacgacaatt cacctgattc
    36301 atggcggtga agatccggtt atcgatctgg cgcatgcggt tgcggcgcag gaagcgttaa
    36361 tcagtgccgg tggtgacgtg acgctggata tcgtggagga tttaggccat gcgatcgaca
    36421 atcgcagtat gcaatttgcc ctggatcact tgcgttatac cattccgaaa cattatttcg
    36481 acgaagcgct aagtggcggt aagcctggcg atgatgatgt gattgaaatg atgtaaaaga
    36541 cagattacct ggcctgataa gacgcgtgag cgtcgcatca ggcatcgttc accattgccg
    36601 gatgcggcgt taacgcctta tccggcctac ggttgagcac tcagcttact tcttcggttg
    36661 atcctttttc ggccagtcgt cgtcatcgtc ccacttatcg ttgaaatcac gatgcggggg
    36721 aagctccggc ttgttggcga gaaatttctt atggtcgacg cgcttgagat ctttaatcac
    36781 gttcagcagg acgcctacca aaaagacaat caccagaatc caccaatatt tagccagcca
    36841 gtccatgctt atttcctctt gcagaaccac tcatcacgcg acgagctgtt ccatgatacg
    36901 ttgatacata cgggcaagta gctgcaggtc ggcagcgttc acacattcat taattttatg
    36961 aatagtggca ttgaccggcc cgagttccac cacctgcgcc cccatgcggg caataaagcg
    37021 cccgtcggac gttccgcctg tggtcagtag ctgcggttta atttcattat agtgctcaac
    37081 cgcgttaacg accgcatcca ccagtttacc gcgcgcggtc aaaaatggct gcccggaaag
    37141 ccaccaatcc accgtatagc gcagttgatg tttttcaagc agggcaagca cctgcgcttt
    37201 gatcatctca tcagtcagtt cggtgctgaa gcggaagtta aactgcacaa acagttcacc
    37261 cggaataacg ttgttactgc ccgttcccgc ctgaatattg gcaatctgca tactggtcgc
    37321 cgggaaaaat tcattgccct gatcccactc aatagccact aattcattaa ggaaaggtgc
    37381 tgcgcgatgt accggattgt cagccagatg tgggtaggca acatgcccct gaacgccatg
    37441 aatggtaagg ttgcaggtta atgatccgcg acgaccattt ttcaccacat cacctaccac
    37501 ttcgatactc gacggttcgc caaccaggca gtaatcgaga cgctcattac gtgccattaa
    37561 cgcttcgacg acttttaccg taccgttgtg ggcactggct tcttcatcag aggtgatcag
    37621 aaatgccagt cgccccgtat ggttgggatg ttgtgcgaca aaacgttctg ccgctaccac
    37681 catcgccgcc agcgagcctt tcatatctgc cgcaccgcgc ccgaataaca tgccgtcacg
    37741 aatggttggt tcaaacggcg gattgatcca acgatcggcg tcgccaggcg gcaccacgtc
    37801 ggtatgcccg gcaaaggcta acgtttcacc ttgcccacgc catgcccaaa aattctgcgt
    37861 atcggcaaag tccatgcgtt caacggtaaa accgatcgcc tgcaaacgtt caatcaacaa
    37921 agcctggcat cctgcgtcat caggactcag ggaagggcgg cgaataagct gttgtgtcag
    37981 ctcaataacc gggcacgaca tagactacac ctcatggaaa aattgctgat aactggaatc
    38041 actgaaaccc agcagcatag gcttaccagg cgcgcagagc aatggacgtt tgataattgc
    38101 aggcatttca gtcattaatg ccgccgcaga ggccgcatcg gtgattttat tgcgggtggt
    38161 ttcgtccagt ttacgccagg ttgtaccacg ggtgttcagt aacgcttccc agcctaattc
    38221 gttgataaaa ccgttcaata attcgctgtc cagcccatcg acgcggtaat catgaaaacg
    38281 atagtcgatg ttattggctt ccagccaacg gcgagccttt ttaatggtgt cacaattttt
    38341 gatgccgtaa agtgtaacca taatgaatcc tttttcgatt tatgagaata aatactcatt
    38401 taagggtgaa aacattttgc ctgcaaatga ttatca
//
LOCUS       DAZFRN010000031        38217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFRN010000031 DAZFRN010000000
VERSION     DAZFRN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.031"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(29..346)
                     /gene="metJ"
                     /locus_tag="ACHKUB_001522"
     CDS             complement(29..346)
                     /gene="metJ"
                     /locus_tag="ACHKUB_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO6654558.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            623..1783
                     /gene="metB"
                     /locus_tag="ACHKUB_001523"
     CDS             623..1783
                     /gene="metB"
                     /locus_tag="ACHKUB_001523"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO6654559.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALADKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            1786..4218
                     /gene="metL"
                     /locus_tag="ACHKUB_001524"
     CDS             1786..4218
                     /gene="metL"
                     /locus_tag="ACHKUB_001524"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO6654560.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(4357..4491)
                     /locus_tag="ACHKUB_001525"
     CDS             complement(4357..4491)
                     /locus_tag="ACHKUB_001525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654561.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            4567..5457
                     /gene="metF"
                     /locus_tag="ACHKUB_001526"
     CDS             4567..5457
                     /gene="metF"
                     /locus_tag="ACHKUB_001526"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO6654562.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEIYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5786..7966
                     /gene="katG"
                     /locus_tag="ACHKUB_001527"
     CDS             5786..7966
                     /gene="katG"
                     /locus_tag="ACHKUB_001527"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO6654563.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGGGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            8060..8965
                     /gene="yijE"
                     /locus_tag="ACHKUB_001528"
     CDS             8060..8965
                     /gene="yijE"
                     /locus_tag="ACHKUB_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO6654564.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(8992..9609)
                     /gene="yijF"
                     /locus_tag="ACHKUB_001529"
     CDS             complement(8992..9609)
                     /gene="yijF"
                     /locus_tag="ACHKUB_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO6654565.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="ACHKUB_001530"
     CDS             complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="ACHKUB_001530"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO6654566.1"
                     /translation="MDHIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFT
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="ACHKUB_001531"
     CDS             complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="ACHKUB_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6654567.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="ACHKUB_001532"
     CDS             complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="ACHKUB_001532"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6654568.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEETDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            14482..15561
                     /gene="frwC"
                     /locus_tag="ACHKUB_001533"
     CDS             14482..15561
                     /gene="frwC"
                     /locus_tag="ACHKUB_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO6654569.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15576..15896
                     /gene="frwB"
                     /locus_tag="ACHKUB_001534"
     CDS             15576..15896
                     /gene="frwB"
                     /locus_tag="ACHKUB_001534"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6654570.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            15947..18244
                     /gene="pflD"
                     /locus_tag="ACHKUB_001535"
     CDS             15947..18244
                     /gene="pflD"
                     /locus_tag="ACHKUB_001535"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6654571.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            18210..19088
                     /gene="pflC"
                     /locus_tag="ACHKUB_001536"
     CDS             18210..19088
                     /gene="pflC"
                     /locus_tag="ACHKUB_001536"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO6654572.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            19090..19431
                     /gene="frwD"
                     /locus_tag="ACHKUB_001537"
     CDS             19090..19431
                     /gene="frwD"
                     /locus_tag="ACHKUB_001537"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6654573.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19418..20269)
                     /gene="yijO"
                     /locus_tag="ACHKUB_001538"
     CDS             complement(19418..20269)
                     /gene="yijO"
                     /locus_tag="ACHKUB_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6654574.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(20484..22217)
                     /gene="eptC"
                     /locus_tag="ACHKUB_001539"
     CDS             complement(20484..22217)
                     /gene="eptC"
                     /locus_tag="ACHKUB_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO6654575.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(22400..25051)
                     /gene="ppc"
                     /locus_tag="ACHKUB_001540"
     CDS             complement(22400..25051)
                     /gene="ppc"
                     /locus_tag="ACHKUB_001540"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO6654576.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(25403..26554)
                     /gene="argE"
                     /locus_tag="ACHKUB_001541"
     CDS             complement(25403..26554)
                     /gene="argE"
                     /locus_tag="ACHKUB_001541"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO6654577.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            26708..27712
                     /gene="argC"
                     /locus_tag="ACHKUB_001542"
     CDS             26708..27712
                     /gene="argC"
                     /locus_tag="ACHKUB_001542"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO6654578.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            27720..28496
                     /gene="argB"
                     /locus_tag="ACHKUB_001543"
     CDS             27720..28496
                     /gene="argB"
                     /locus_tag="ACHKUB_001543"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO6654579.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            28557..29930
                     /gene="argH"
                     /locus_tag="ACHKUB_001544"
     CDS             28557..29930
                     /gene="argH"
                     /locus_tag="ACHKUB_001544"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO6654580.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            30196..31113
                     /gene="oxyR"
                     /locus_tag="ACHKUB_001545"
     CDS             30196..31113
                     /gene="oxyR"
                     /locus_tag="ACHKUB_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO6654581.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(31096..32496)
                     /gene="sthA"
                     /locus_tag="ACHKUB_001546"
     CDS             complement(31096..32496)
                     /gene="sthA"
                     /locus_tag="ACHKUB_001546"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO6654582.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            32827..33477
                     /gene="fabR"
                     /locus_tag="ACHKUB_001547"
     CDS             32827..33477
                     /gene="fabR"
                     /locus_tag="ACHKUB_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO6654583.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDIGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            33477..33836
                     /gene="yijD"
                     /locus_tag="ACHKUB_001548"
     CDS             33477..33836
                     /gene="yijD"
                     /locus_tag="ACHKUB_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO6654584.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(33876..34976)
                     /gene="trmA"
                     /locus_tag="ACHKUB_001549"
     CDS             complement(33876..34976)
                     /gene="trmA"
                     /locus_tag="ACHKUB_001549"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO6654585.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      35090..35280
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            35345..37189
                     /gene="btuB"
                     /locus_tag="ACHKUB_001550"
     CDS             35345..37189
                     /gene="btuB"
                     /locus_tag="ACHKUB_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO6654586.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            37134..37991
                     /gene="murI"
                     /locus_tag="ACHKUB_001551"
     CDS             37134..37991
                     /gene="murI"
                     /locus_tag="ACHKUB_001551"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO6654587.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         8659 a         9652 c        10608 g         9298 t
ORIGIN      
        1 gttccactcc gcgccgctct tttccgcttt agtattccca cgtctccggg ttaatcccca
       61 tctcacgcat gatctctttt gccgcttccg ggatttcgtc gctgcgctct ttacgcagat
      121 cggcatcatc cggcaaaggt tgcccggtaa aggcatgcag aaacgcttcg cacagcagct
      181 cgctgttggt agcgtgacgc aggttgttca cctgacgacg cgtgcgttca tcggtgagga
      241 tttttaacac cttaagagga atggaaaccg taatcttttt gacttgttca ctcttcttgc
      301 cgtgctcagc gtatgggctg atatattcgc cgctccattc agccatgaga tacttaatcc
      361 tcttcgtcaa taaattgaga ccagaccaca caatttatgt gggtactgac cgtaaacccg
      421 catagtttac cgtacaggcg ttaccgtgac atcgtgtaat gcacctgtcg gcgtgataat
      481 gcatataatt ttaacggcta tttgggattt gctcaatcta tacgcaaaga agtttagatg
      541 tccagatgta ttgacgtcca ttaacacaat gtttactctg gtgcctgaca tttcaccgac
      601 aaagcccagg gaacttcatc acatgacgcg taaacaggcc accatcgcag tgcgtagcgg
      661 gttaaatgac gacgaacagt atggttgcgt tgtcccaccg atccatcttt ccagcacata
      721 taactttacc ggatttaatg aaccgcgcgc gcatgattac tcgcgtcgcg gcaacccaac
      781 gcgcgatgtg gttcagcgtg cgctggcaga actggaaggc ggtgctggtg cagtacttac
      841 taataccggc atgtccgcga ttcacctggt aacgaccgtc tttttgaaac ctggcgatct
      901 gctggttgcg ccgcacgact gctacggcgg tagctatcgc ctgttcgaca gcctggcgaa
      961 acgcggttgc tatcgcgtgt tgtttgttga tcaaggcgat gaacaggcat tacgggcagc
     1021 gctggcagat aaacccaaac tggtactggt agaaagccca agtaatccat tgttacgcgt
     1081 cgtggatatt gcgaaaatct gccatctggc aagggaagtc ggggcggtga gcgtggtgga
     1141 taacaccttc ttaagcccgg cattacaaaa tccgctggca ttaggtgccg atctggtgtt
     1201 gcattcatgc acgaaatatc tgaacggtca ctcagacgtg gtggccggcg tggtgattgc
     1261 taaagacccg gacgttgtca ctgaacttgc ctggtgggca aacaatattg gcgtgacggg
     1321 cggcgcgttt gacagctatc tgctgctacg cgggttgcga acgctggtgc cgcgtatgga
     1381 gctggcgcag cgcaacgcgc aggcgattgt gaaatatctg caaacccagc cgttggtgaa
     1441 aaaactgtat cacccgtcgc tgccggaaaa tcaggggcat gaaattgccg cgcgtcagca
     1501 aaaaggcttt ggcgcaatgt tgagttttga actggatggc gatgagcaga cgctgcgtcg
     1561 tttcctgggc gggctgtcgt tgtttacgct ggcggaatca ttagggggag tggaaagttt
     1621 aatctctcac gccgcaacca tgacacatgc aggcatggca ccagaagcgc gtgctgccgc
     1681 cgggatttcc gagacgctgc tgcgtatctc caccggtatt gaagatggcg aagatttaat
     1741 tgccgacctg gaaaatggct tccgggctgc aaacaagggg taaaaatgag tgtgattgcg
     1801 caggcagggg cgaaaggtcg tcagctgcat aaatttggtg gcagtagtct ggctgatgtg
     1861 aagtgttatt tgcgtgtcgc gggcattatg gcggagtact ctcagcctga cgatatgatg
     1921 gtggtttccg ccgccggtag caccactaac cagttgatta actggttgaa actaagccag
     1981 accgatcgtc tctctgcgca tcaggttcaa caaacgctgc gtcgctatca gtgcgatctg
     2041 attagcggtc tgctacccgc tgaagaagcc gatagcctca ttagcgcttt tgtcagcgac
     2101 cttgagcgcc tggcggcgct gctcgacagc ggtattaacg acgcagtgta tgcggaagtg
     2161 gtggggcacg gtgaagtgtg gtcggcacgc ctgatgtctg cggtacttaa tcaacaaggg
     2221 ctgccagcgg cctggcttga tgcccgcgag tttttacgcg ctgaacgcgc cgcacaaccg
     2281 caggttgatg aagggctttc ttacccgttg ctgcaacagc tgctggtgca acatccgggc
     2341 aaacgtctgg tggtgaccgg atttatcagc cgcaacaacg ccggtgaaac ggtgctgctg
     2401 gggcgtaacg gttccgacta ttccgcgaca caaatcggtg cgctggcggg tgtttctcgc
     2461 gtaaccatct ggagcgacgt cgccggggta tacagtgccg acccgcgtaa agtgaaagat
     2521 gcctgcctgc tgccgttgct gcgtctggat gaggccagcg aactggcgcg cctggcggct
     2581 cccgttcttc acgcccgtac tttacagccg gtttctggca gcgaaatcga cctgcaactg
     2641 cgctgtagct acacgccgga tcaaggttcc acgcgcattg aacgcgtgct ggcctccggt
     2701 actggtgcgc gtattgtcac cagccacgat gatgtctgtt tgattgagtt tcaggtgccc
     2761 gccagtcagg atttcaaact ggcgcataaa gagatcgacc aaatcctgaa acgcgcgcag
     2821 gtacgcccgc tggcggttgg cgtacataac gatcgccagt tgctgcaatt ttgctacacc
     2881 tcagaagtgg ccgacagtgc gctgaaaatc ctcgacgaag cgggattacc tggcgaactg
     2941 cgcctgcgtc aggggctggc gctggtggcg atggtcggtg caggcgtcac ccgtaacccg
     3001 ctgcattgcc accgcttctg gcagcaactg aaaggccagc cggtcgaatt tacctggcag
     3061 tccgatgacg gcatcagcct ggtggcagta ctgcgcaccg gcccgaccga aagcctgatt
     3121 caggggctgc atcagtccgt cttccgcgca gaaaaacgca tcggcctggt attgttcggt
     3181 aagggcaata tcggttcccg ttggctggaa ctgttcgccc gtgagcagag cacgctttcg
     3241 gcacgtaccg gctttgagtt tgtgctggca ggtgtggtgg acagccgccg cagcctgttg
     3301 agctatgacg ggctggacgc cagccgcgcg ttagccttct tcaacgatga agcggttgag
     3361 caggatgaag agtcgttgtt cctgtggatg cgcgcccatc cgtatgatga tttagtggtg
     3421 ctggacgtta ccgccagcca gcagcttgct gatcagtatc ttgatttcgc cagccacggt
     3481 ttccacgtta tcagcgccaa caaactggcg ggagccagcg acagcaataa atatcgccag
     3541 atccacgacg ccttcgaaaa aaccgggcgt cactggctgt acaatgccac cgtcggtgcg
     3601 ggcttgccga tcaaccacac cgtgcgcgat ctgatcgaca gcggcgatac tattttgtcg
     3661 atcagcggga tcttctccgg cacgctctcc tggctgttcc tgcaattcga cggtagcgtg
     3721 ccgtttaccg agctggtgga tcaggcgtgg cagcagggct taaccgagcc tgatccgcgt
     3781 gacgacctct ccggcaaaga cgtaatgcgc aagctggtaa ttctggcgcg tgaagcgggt
     3841 tacaacattg aaccggacca ggtacgtgtg gaatcgctgg tgcctgctca ttgcgaaggc
     3901 ggcagcatcg accatttctt tgaaaatggc gatgaactta acgagcagat ggtgcaacgg
     3961 ctggaagcgg cccgcgaaat ggggctggtg ctgcgctacg tggcgcgttt cgatgccaac
     4021 ggcaaagcgc gtgtgggcgt ggaggcggtg cgtgaagacc atccgctggc atcactgctg
     4081 ccgtgcgata acgtctttgc catcgaaagc cgctggtatc gcgacaaccc gctggtgatc
     4141 cgtgggcctg gcgcggggcg cgacgtcacc gccggggcga ttcagtcgga tatcaaccgg
     4201 ctggcacagt tgttgtaatt tcagaaattt aataatgccc ggtactcatg ttttcgggtt
     4261 tatggtttct aatgaaatat attgaattat cataggatta ggccggatta agcgtttacg
     4321 acgaatccgg caagaagcaa taagtacatg gttagtttat atttgcagtc cggtttgctt
     4381 tgcataccgg attttctttt tcttaccatc ctgaagtttt ttcatcttcc ctgatttttc
     4441 ctcaccatca ttggtcattt ttcggttgac gcccttcggc ttttccttca tctttacatc
     4501 tggacgtcta aacggataga tgtgcacaac acaacatata actacaagcg attgatgagg
     4561 taaggtatga gcttttttca cgccagccag cgggatgccc tgaatcagag cctggcagaa
     4621 gtccaggggc aaattaacgt ttcgttcgag tttttcccgc cgcgtaccag tgaaatggag
     4681 cagaccctgt ggaactccat cgatcgcctt agcagcctga aaccgaagtt tgtatcggtg
     4741 acctatggcg cgaactccgg cgagcgcgac cgtacgcaca gcattattaa aggcattaaa
     4801 gatcgcactg gtctggaagc ggcaccgcat cttacttgca ttgatgcgac gcccgacgag
     4861 ctgcgcacca ttgcacgcga ctactggaat aacggtattc gtcatatcgt ggcgctgcgt
     4921 ggcgatctgc cgccgggaag tggtaagccg gaaatatatg cttctgacct ggtgacgctg
     4981 ttaaaagaag tggcagattt cgatatctcc gtggcggcgt atccggaggt tcacccggaa
     5041 gcaaaaagcg ctcaggcgga tttgctcaac ctgaaacgca aagtcgatgc cggagccaac
     5101 cgcgcgatta ctcagttctt ctttgatgtc gaaagctacc tgcgttttcg tgaccgctgc
     5161 gtatcggcgg gtattgatgt ggaaattatt ccgggaattt tgccggtctc gaactttaaa
     5221 caagcgaaga aatttgccga tatgaccaac gtgcgtattc cggcgtggat ggcacaaatg
     5281 tttgacgggc tggacgacga tgccgaaacc cgcaaactgg tgggagcgaa tattgccatg
     5341 gatatggtga agattttaag ccgtgaagga gtgaaagatt tccacttcta tacgcttaac
     5401 cgcgctgaaa tgagttacgc gatttgccat acgctgggtg ttcgacctgg tttataaata
     5461 gtgtggcttt tgtgaaaatc acacagtgat cacaaatttt aaacagagca caaaatgctg
     5521 cctcgaaatg agggcgggaa aataaggttg tcagccttgt tttctccctc attacttgaa
     5581 ggatatgaag ctaaaaccct tttttataaa gcatttgtcc gaattcggac ataatcaaaa
     5641 aagcttaatt acgatcaatt tgacctacat ctctttaacc aacaatatgt aagatctcaa
     5701 ctatcgcatc cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg
     5761 taacactgta gaggggagca cattgatgag cacgtcagac gatatccata acacgacagc
     5821 cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac
     5881 cactcgcgac tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg
     5941 ttctaacccg ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta
     6001 cggcctgaaa aaagatctga aagccctgct gacagaatct cagccgtggt ggcctgccga
     6061 ctggggcagc tacgccggtc tgtttattcg tatggcctgg cacggcgcgg ggacttaccg
     6121 ttcaatcgat ggacgcggcg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc
     6181 ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg ttgtggccaa tcaaacagaa
     6241 atatggtcag aaaatctcct gggccgacct gtttatcctc gcgggtaacg tggcgctgga
     6301 aaactccggc ttccgtacct tcggttttgg tggcggtcgt gaagacgtct gggaaccgga
     6361 tctggatgtt aactggggtg atgaaaaagc ctggctgacc caccgtcatc cggaagcgct
     6421 ggcgaaagca ccgctgggtg caaccgagat gggcctgatt tacgttaacc cggaaggccc
     6481 ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgtgcgacct tcggcaacat
     6541 gggcatgaac gacgaagaaa ccgtggcgct tattgcgggt ggtcatacgc tgggtaaaac
     6601 ccacggtgcc ggtccgacat caaatgtagg tcctgatcca gaagctgcac cgattgaaga
     6661 acaaggttta ggttgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc
     6721 tggtctggaa gtagtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa
     6781 cctgttcaag tatgagtggg tacagacccg cagcccggct ggcgcaatcc agttcgaagc
     6841 ggtagacgca ccggaaatta tcccggatcc gtttgatccg tcgaagaaac gtaaaccgac
     6901 gatgctggtg accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg
     6961 tttcctcaac gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac
     7021 gcacagggat atggggccga aatctcgcta catcgggccg gaagtgccga aagaagatct
     7081 gatctggcaa gatccgctgc cgcagccgat ctacaacccg accgagcagg acattatcga
     7141 tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg
     7201 ggcttctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct
     7261 ggcattaatg ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt
     7321 tctggagaaa atccagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc
     7381 tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt ttgagcattc atgtaccgtt
     7441 tgcgccgggt cgcgttgatg cgcgtcagga tcagactgac attgagatgt ttgagctgct
     7501 ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggacgttt ccaccaccga
     7561 gtcactgctg atcgacaaag cacagcaact gacgctgacc gcgccggaaa tgactgcgct
     7621 ggtgggcggg atgcgtgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac
     7681 tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga
     7741 gtggaaagcg actgacgaat cgaaagagct gttcgaaggt cgtgaccgtg aaaccggcga
     7801 agtgaaatac acagccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc
     7861 ggtagcggaa gtttacgcca gcagcgatgc ccacgagaag tttgttaaag acttcgtggc
     7921 ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca
     7981 gcgactgtct ttcaggcagt cgctgaagtt tctttaccgg cgtatagtgt ccacaggaaa
     8041 actacacact ggatctctca tgtctgccgc aggaaagagc aacccactgg caatcagtgg
     8101 cctggttgtg ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag
     8161 ctacatcggt gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt
     8221 catcgtcctt ttattacgtg gtcgcggaat gcgcccgaca ccgtttaaat acaccttagc
     8281 cattgccctg ttacaaacct gcgggatggt tggcctggcg cagtgggcgc tggtcagcgg
     8341 cggagcgggg aaggtggcga tcctaagcta taccatgccg ttctgggtgg tgattttcgc
     8401 cgcgttgttt ctcggtgaac gcctgcgacg tgggcaatat ttcgcgatac tgattgccgc
     8461 tttcggctta tttttggtgt tgcagccgtg gcaactcgat ttctcttcaa tgaaaagtgc
     8521 catgctggca atcctctccg gcgtcagttg gggggcgagc gcgattgttg ctaaacgcct
     8581 atacacccgt catccgcgcg tggatttatt atcgttaaca tcctggcaga tgctctatgc
     8641 ggcgctggtg atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc
     8701 caccgtgttc tgggcgctgg cctacagtgc gattctggcg acggcgctgg cgtggagctt
     8761 atggttgttt gtattgaaaa acttgcctgc cagtattgcc agcttaagca cgctggccgt
     8821 tcccgtttgc ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga
     8881 aggtagcggt attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga
     8941 agctgtcagt gtaaaaagca tctgaatttt ttcttcatgt ggggcgatcg cttatttaac
     9001 aaaataacga taatgcccca ccatccgcca gctaaacagc acatcttctt cctgcgcgcc
     9061 tgcgccaatg ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgacaccac
     9121 cccaatatgt gccagtccgt tatccagtcg ccaggagaca atatcgcccg cttgatagtc
     9181 actggggttc ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg tttccagatt
     9241 aggcacccga cggtgatcga tgttgctgtc cgggcgcttt aactgccatt tttgcgggta
     9301 ctcggcaaaa ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga ccttctggct
     9361 gcgcaatgca cggatcacca catcggaaca taccccgcgt tcttgcggga catcaccgcc
     9421 aggataggta agctgcatat acgccggatc gtaaaatagc gtgctgccga tttgctgtct
     9481 ggcaccgtct gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaac
     9541 ggcgggagat tttaaggagt ggctggtaaa tgccgttagc aggctgagca gcgccagtga
     9601 agctttcatc tggttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg
     9661 attgttctgt ggagaaaatg tccagaagcg gataaaaaac tcctgaacaa cgtgggagtt
     9721 tcagattact ggcacagagt gccggatgcg gagtgaacgc cttatccggc ctacaaaagc
     9781 acgcatattc aatatattgc agagatttcg taggcttgat aaacgtagcg catcaggcaa
     9841 ttttgcgttc aaactcccgg acaagccggg agtttggagt aggttattcc cactcttgca
     9901 ggaaacgctg accgtactgg tcggctacca gcagagcggc gtaaacctga tctggcgtcg
     9961 cgccgccagg catgttgtga atggtttcac cttctgcaca tgccgcttct gccacaattc
    10021 gcattttcgc cgggacatct tctttaatat ccagttgagc gagagttatt ggcaaaccta
    10081 ccgcatggct aagtgcagct acggtttcga tttcctcaac cggtgcgttt tccagaacca
    10141 gctgcgtcag cgtaccaaat gccacttttt caccgtgata atagtgatgc gcgtccggga
    10201 tagcggtcag gccgttatgc accgcgtgag ccgccgccag cccgccgctt tcaaaaccaa
    10261 cgccgctcaa ataggtgttc gcttcaatca cgcgctccag cgccggggtt actacatgct
    10321 gttcggcggc aagcatcgct ttttcgcctt cttccagcag ggtgttgtag cacagttcag
    10381 ccagtgccag cgccgcctgg gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag
    10441 agcaagcacg cgcttcaaac caggttgcca gcgcatcgcc gatacccgct gctaacagac
    10501 gtgcaggtgc gcccgcgacg attttggtgt cgacaatgac catattcggg ttatttggca
    10561 acagcagata gcggtcaaac tcaccctcat cggtgtagat aacagacaat gcgctgcacg
    10621 gtgcatcggt agaggcgata gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca
    10681 gtgctttggc agtatcgagg gtttttccgc caccgatacc gagaattgcg ccacactgcg
    10741 cagtctccgc gatgccacgc agacggtcga tctcattttg cgaacattca ccgccaaacg
    10801 gcgcaatttc tactaccagt ccagcatctt taaagctttt ctcgacagtg gattgagtaa
    10861 aacctaaaac aaatttgtca cccaccacta accagcgttc tgccagcggc ttcaggtatt
    10921 cgcccagacg attaatcaca tcagcgccct ggatgtattt acccggtgat tgaataatgt
    10981 ggtccataat tgctccttta gagatgagta gtgccaaatg cggcattcca gtcgtgttcg
    11041 aacttctcta tagctgactc taccgcaggg gtgttgagca tttgttgcgc tacatctaag
    11101 ggcagggtga tggattcaca tcctgccagt aaacagtcca gcgcctgacg cggcgtttta
    11161 aagctggccg ccagcaccat gctttctggt gcgtgcattt ctaacagcgc ttgcagctcc
    11221 tgaaccgtac gaatgccgtc cccgccctgg gcatctacgc ggttaacata cggagcaacg
    11281 tattttgccc ctgccagcgc ggccagtaac ccttgtgcag cactgtacac tgcggtgcca
    11341 agcgtagtaa taccctcttt tttcagcatc ttaatggcgg ccagaccttc ggaagtcacc
    11401 gggattttca ccacaatgcc cggaatggcg tcgcgcaggc gcttcgcttc ttccaccata
    11461 ccctgcgcgt cacgactcat agtctgagca aacagaatgc cctcatcacc aatcgctttt
    11521 tgcagacgcg gcagaacttc ccatatggac tccttgctgg cagcgataat gctcgggtta
    11581 gttgtcaccc cggcaatggg gaatatgcgt gccagacgtt cgacttctgc gacgttagcg
    11641 gtgtccagat acagttccat gatgtttcct tttacagttc cagttcatgt tgcagcaggc
    11701 tggcgatagc gtcggcagag gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga
    11761 tgcggcgagc gaggcgcgag aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca
    11821 gggttaacat aatgatgaat tgcgcttcat catcgcccca gcgcaccggc gcttgcagac
    11881 gcgccacgct gatggtggat tgctcaatgt gttctgattt gctgtgtgga atggcaaaac
    11941 taaagcccag accggtagag aaaacggcct cgcgcgccca taagtcggct tccagtttac
    12001 gcggatagcg gcagcggccc gccagcagca ggttatcggt catgcctttg agcacttctt
    12061 ctttgctgcg ccagtcactt tccagcgtga tgcactcggc ggtgaccagc ggtgcgtcct
    12121 gttgggtcat gcggaattgc gccagcaggt gttccacttc cagcgaagta cggcaggcca
    12181 ttgcttggtt gagcaacttg cggcactcac ggctatcaag ttgcgccatc cgcgctttcg
    12241 ccgccggaat tgatggtgcg ctcatgctga gttcatccag ccctaagccg accagcaacg
    12301 gcagcacgga acctttcgct cccagctcac cgcacagacc aatccatttg ccctggcgat
    12361 gcaccgcctg cacggcgtaa tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt
    12421 gacgagtaac cttagcgtta tcgcgatcca ctgccagcag atattgcgtc aggtcattac
    12481 taccaatact aaagaaatca gtctcttcgc agcattgatc gatgatgaac atcaccgacg
    12541 gtacttccag cataatgcca agctggatct tctcatcaaa cggaatgtgt tcgttacgca
    12601 gttgctgttt ggcttccgcc agtttttctt tcacccataa gatctcttcc attgaggaga
    12661 tcatcgggat catgattttc aggctgccgt gagcggaggc gcgcaggatc gaccgtagtt
    12721 gtgtggtgaa cagcgaggcg tattcttcat aaatacgcac agcgcgataa ccgaggaacg
    12781 ggttagcttc tgccggaatg ttcagataat caacgggttt gtcgccgcca atgtccatag
    12841 tgcgcacaat aatgctgcgt ccgttggcag actccagcgc ctgacaaaaa atgttgtaca
    12901 gctcgctttc gcccggtgca ctggtgcgat ccatatagag catttcagtg cggaacaaac
    12961 caacgccttc cgcaccattg ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag
    13021 cggcaatttc aatgcggata ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct
    13081 gttgctcacg cagggcgtcc tgtacgcggg cttcttgctg ataataacga gctacggctt
    13141 ctcctggctc aaccacaatc gccccagcgt tgccgtcgat ataaatcgtt tgatgctgcc
    13201 acggagtaag ggcatcaata tctacaccaa ccagtgtcgg aatgttgaac gaacgggcaa
    13261 gaattacggt atgtgaggtg gtgccgccac ttttcagtaa cagacctttg aggtgatttt
    13321 tatccagttc gaggaactgg ctgggagtca gttcatcagc catacaaatg gcgggctgcg
    13381 tcagtttgcc cggtgccggg aagcgttgct caccgtagat ttgctggagt aactggaagc
    13441 agacgtcgcg tacgtccagg gcacgttctt gcaggtagct gctgctggaa cgggcgaact
    13501 cttcgcaaaa gtgattcgca ctggtgacaa tcgcttcggc gcagcttaat ccggcgctga
    13561 cgcctgccag taaatgttcg cgcagggaag tatcgccagc cagggatcgg tgcgcttcca
    13621 gaatcgcgct ggtggcaccg tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca
    13681 gcgtcaggcc gttttccagt gcggattgct cggcgtcaac gcttttggct gcgggaagat
    13741 tacccagcgc attgagatct aaagaagaga tcggcgtcag aatgccgcct gcactaccgc
    13801 tgcacacggt gcgggcgcgg ataatttgcg gattcagatt ggtcagtgaa accggcagtg
    13861 gttccagttc gtcagattta acttccgcca gcggcgcatc gcagtggggg aattcatcgc
    13921 gcagccattg gcttaaacgc tggtgcgctt cctgttcgtc ggccccggaa atcagtaact
    13981 ggcagttatc gcccgccagc gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc
    14041 ccttgcggtc agtgcgcagg ttatgccact caatttgtga tgaaaaagta ttacacagcg
    14101 tttcaacgtg gcttgccgga cgtgcatgta cgccgttagg tagctcacaa ataaattcca
    14161 caatcagggc cattgcctct ctcccggtac ggttttctgt ttacaggata agagcgcacg
    14221 gcaacggcct gccatgtgac aaatctgcta aaagctggac aaatgtaatg taaccgtcaa
    14281 tttgcgacgc gtctcacaag acgctgtttt gcggcatgct tccggtttat cgcaagttat
    14341 gaggcggatc gcatttttgt actgatatta caaaagtcca gtaaatggcc tttttatcca
    14401 ctgtttgctc cgcctgcgat tgcctattgt ttcctccatc aaaaatatgg acatgggctt
    14461 tcgcaagccc gggagcagct tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag
    14521 catttaatga cgggcgtttc acacatgatt cccttcgtgg tatcgggcgg tattttgctg
    14581 gcggtttccg tcatgttgta tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat
    14641 ctgaaaaaac tgtttgatat cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgca
    14701 gcgtacatcg gttattccat tgcagagcgt tctgcgctgg ctccgtgcgc tatcggtgcc
    14761 tgggttggta acagctttgg tgcgggcttc tttggtgcac tgatcgccgg gattatcggc
    14821 ggcatcgtgg tgcattacct gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg
    14881 cctatcttca ttattcctat cgtcggcaca ctgattaccg caggcatcat gatgtggggc
    14941 ttgggcgagc ctgtaggggc gttgaccaac agcctgactc agtggcttca ggggatgcag
    15001 cagggcagca ttgttatgct ggcggtgatc atgggtctga tgctggcgtt cgatatgggc
    15061 ggtccggtta acaaagtggc ctatgccttc atgctgattt gcgttgctca gggtgtttat
    15121 accgtggtgg ccatcgctgc cgtgggtatt tgtatcccgc cgctggggat ggggctggcg
    15181 acgctgattg gtcgtaaaaa tttctccgca gaagagcgcg aaaccggcaa agcggcactg
    15241 gtgatgggct gtgttggcgt tactgaaggg gcgattcctt tcgccgctgc cgatccgctg
    15301 cgtgtcattc cttccatcat ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc
    15361 ggtgcgcagt gctatgcagg ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag
    15421 ctgggttata tcgcagcagt ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg
    15481 ctgaaaagtc tggcgcgtaa aaatgggtct tcgactgatg aaaaagaaga cgacctggat
    15541 ttggattttg aaattaatta attgaggaac gagccatgac gaaaattatt gcagtaaccg
    15601 catgtccttc aggtgttgcc catacctata tggcagcaga ggcgctggaa agtgccgcga
    15661 aagcgaaagg ctgggaagtg aaagtagaaa cccaggggtc aattggtctg gaaaatgaac
    15721 tgactgcgga agatgtggcg agcgctgata tggtgattct gaccaaagat atcggcatca
    15781 agtttgaaga gcgttttgct ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa
    15841 aacgtgctga cgccattatg agcaaaattg aagctcacct ggcgcaaacc gcttaagttc
    15901 ttctcccgct cgcaagggcg ggttcgcttt cccacaggag ttcctcatga cgaatcgtat
    15961 ctctcgcctc aaaactgcac tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct
    16021 gctttatacc gccagccatc ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa
    16081 agcaacagcg tatatccttg aacatgttga aatttcgatt cgtgatgaag aactgattgc
    16141 cggtaaccgc accgtaaaac cgcgcgctgg gattatgtcg ccggaaatgg acccttactg
    16201 gctgctgaaa gagctggatc aattcccgac gcgtccgcag gaccgctttg ctatcagtga
    16261 agaagataaa cgtatctacc gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa
    16321 agatttcatc aacgggcaga tgacagatga agtaaaagcc gcgaccagca cgcagatttt
    16381 cagcatcaac cagacagata aaggccaggg gcacattatt attgattacc cacgcctgct
    16441 gaatcacggg ctgggggagc tggtggcgca gatgcagcaa cattgtcagc aacagccgga
    16501 gaatcacttt tatcaggccg cgctgttact gctggaagcc tcgcagaaac acattctgcg
    16561 ttacgctgaa ctggcggaaa caatggcggc aaactgcaca gatgcccagc gtcgcgaaga
    16621 actgctgact attgcggaga tctcccgcca taacgccgaa cataagccgc agacgttctg
    16681 gcaggcgtgc cagttattct ggtacatgaa catcattctg caatacgaat ccaacgccag
    16741 ttcgctatcg ttggggcgct tcgaccagta tatgttgccg ttctatcagg catctttaac
    16801 ccagggcgaa gatccggcgt tcctgaaaga actactcgaa tctttatggg tgaaatgcaa
    16861 cgacatcgtg ctgttgcgct ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg
    16921 ctataccgca ctgctcggcg ggttaaccga gaacggacgt agcgcggtga acgtgctttc
    16981 gttcctttgc cttgacgcct atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg
    17041 cactaacgcc ttgatcgaca cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg
    17101 caccggtatt ccgcaaatct ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg
    17161 cgtttcgctg gaagatgcgc gcgactattc cgtagtgggc tgtgtggaat tatctattcc
    17221 cggcagaacc tacggcttgc atgacatcgc gatgtttaat ctgctgaaag tgatggaaat
    17281 ctgcctgcat gaaaatgaag gcaatgctgc gctgacttat gaaggtttac tggagcagat
    17341 ccgcgccaag atcagccact acatcaccct gatggttgag ggcagcaata tttgtgatat
    17401 cggccatcgc gactgggcac ctgtaccgct gctctcgtct tttatcagcg attgtctgga
    17461 aaaaggtcgc gatattaccg atggcggcgc gcgttataac ttctccggcg tacaggggat
    17521 cggtatcgcc aacctgagcg attctctcca tgcgttgaaa gggatggttt ttgagcaaca
    17581 gcgtttaagt tttgacgaat tgctgtcggt attaaaagcc aactttgcaa cgccagaagg
    17641 cgaaaaagtc cgtgctcgct taattaaccg cttcgagaaa tacggtaacg atatcgacga
    17701 ggtggataac atcagcgccg aactgttacg ccactactgc aaagaagtgg aaaaatacca
    17761 gaacccgcgc ggtggttact tcacgccggg atcgtatacc gtttctgctc acgtcccgtt
    17821 gggatcggtg gttggcgcga cgccagacgg tcgttttgcc ggggaacaac tggcagacgg
    17881 cggcttgtca cctatgctgg gccaggatgc acaagggcca acagcggtac tgaagtcagt
    17941 cagtaagctc gataatacgc tgctgtctaa cggtacgttg ctgaacgtga aattcactcc
    18001 ggcgaccctg gaaggtgaag cgggattacg caaactggcc gacttcttac gggcgtttac
    18061 ccagcttaag ttgcagcata ttcagtttaa cgtggtgaac gccgacacgt tgcgggaagc
    18121 gcaacagcgc ccacaagatt atgccgggct ggtggtgcgc gttgccggat acagcgcctt
    18181 ctttgtcgaa ctgtcgaagg agatccagga tgacatcatc cgccggacag cgcatcagct
    18241 gtaacgttgt ggaaacgcgc cgcaatgatg tggcgcgcat tttcaacatt cagcgttatt
    18301 cactgaatga cggcgagggc attcgtacgg tggtcttttt taaaggctgt ccgcatcttt
    18361 gcccatggtg tgctaatccg gagtcgatct ccggcaaaat ccagacggta cgcagagagg
    18421 cgaaatgtct gcactgtgcg aaatgtttgc gtgatgcgga tgaatgcccc tccggggcgt
    18481 ttgaacggat tggtcgcgat atcagccttg acgcgctgga acgggaagtg atgaaagatg
    18541 acattttctt tcgcacgtcc ggcggcggcg tcacgctttc tggcggcgaa gtgttaatgc
    18601 aggcggagtt tgctacccgt tttttacagc ggctgcggtt gtggggcgtg tcatgcgcca
    18661 ttgaaactgc cggagacgcg ccagccagca agctgttacc actggcgaaa ttgtgcgatg
    18721 aagtgctgtt cgatttaaaa attatggacg cgactcaggc gcgggatgtg gtgaagatga
    18781 acctgccacg cgtgctggag aatctgcgtt tgctggtgag tgagggtgtc aacgtgatcc
    18841 cgcgtttacc attgatccct ggtttcacgc tcagccggga gaatatgcag caggcgctgg
    18901 atgtactgat cccgctgaaa atcaggcaga tccatctgtt accgtttcat cagtacggcg
    18961 aaccgaaata ccgcctgctg gggaaaacat ggtcgatgaa agaggtgcct gcgccgtcgt
    19021 cagccgatgt gacaacgatg cgcgaaatgg cagaacgggc cggatttcag gttaccgtgg
    19081 gaggttaaaa tggcatacct ggtggcagta accgcctgcg taagcggcgt ggcgcatact
    19141 tatatggcgg cggaacggct ggaaaagttg tgccagttag agaagtgggg cgtcagcatt
    19201 gaaactcagg gcgcgctggg aacggagaat cgtttagcgg acgaggatat ccgtcgggcg
    19261 gatgttgctc tgttgattac ggatatcgag cttgccggtg ccgagcgatt tgaacattgc
    19321 cgctatgtgc aatgcagcat ctacgcattc ctgcgtgagc cgcagcgggt aatgagcgcg
    19381 gtgcgcaaag tgctttctgc gccgcagcaa acccatctta ttctggagta gtcggttttt
    19441 ctgtcagttg gctgtggtac tgccgacgat attccgacgg cgagcgttcg gtgtttttac
    19501 gaaacagacg gcagaagtag ttgctgtcga caaagccgca ggcgtgcgcc acttctttta
    19561 ccttcaggtc atagcctttc aataacgtct tagcgtgctc cagtcgcgtg tgattcaggt
    19621 attcgttaaa accaatggcc cccgtttttt gaaacaggtg cgagaggtaa tttggcgaaa
    19681 tatagaatgc ctgtgcgaca gattcgcggg taagcgcgga ggcatagcgt tcgtcgatat
    19741 aatcgcgaat agcttcgaat agagcctggc tgcgcgaggc ggtctggatt tggctgccga
    19801 gcaaatcgcg gcagtggcta agcaggctgg cgacgataag ccttgccgtt tgctgctcct
    19861 gcggctgcat ttgcatttca ttgagcgttt gtagcagaaa agaacctatg cgtgggccgc
    19921 gccgggcgac gtgttgcttc gccagatttt gatattgttt gccatcccat tgcacgacgc
    19981 tgaagccgag ttgctgtttg ccaaacagca cgctaaaggt agtagcgggg gcttgccatt
    20041 gtggaaaatt ccagccacca gcgggaacgt acagcacatc gccgggaact aacgttgctc
    20101 cagcgccggt atcaacaaac tcaccttcca gcactatttc cagccgtgga aaatcgacct
    20161 gatacgcaag atcgggcgcc ggaccgttac tgctggcaaa gtaaatctgg cgcagggaca
    20221 gcgggccgtt gatcaggcgg gagagcagat agctgacgtc gtgatacatg ttattccctt
    20281 aggtatgtag acgggaaaac agtaactggc attattaaaa aaggccacct gaaaagtgtg
    20341 gcctgagggg agttcgatgt aaacattgcg gcagttgtat tgccggatgt ggcgtaaacg
    20401 ccttattcag tctaccgccg cgatctgtag gccggataag acgcgtcaag cgtcgcatcc
    20461 ggcaaatagc gcctggctga taattactga ttacctacct ggtcgccata cggcagagtg
    20521 tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa tccagcgggt agtttctttg
    20581 aactgcggat tcaccactga acgggttgga tcgtaaccgt cgtaagataa gcccgccaaa
    20641 tctgaccagg tgtggatcag ttccgccagg ctgtatttac gatcgacatc ctgtgagaaa
    20701 tcacggggat gagtcgcttg ccatttttcc gacgtccaca gcaggaacgg aatggtgtac
    20761 atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt tgtgcggcgg cgtgtcgtaa
    20821 acctcttcac cgtggtcaga gaagtaaacc aggaatccgt tcgggtctgc cgctttaaag
    20881 tctttaatca ggctggcaac cacatgatcg ttatacaggt tagcgttgtc ataatcgtta
    20941 tatgattcca gctcttctgc gcttaatcct ggcggaacat gatcggtatt gccatcaaac
    21001 ttgccctgat tttccgggta gcggtatttg tatttgatat gcgtacccag cagatgaacg
    21061 atgatcagtt tcttcggcgc agggtcattc agcacatcct ggaacggctt cagcacgttg
    21121 gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca tgtagtactg cttgtcggtc
    21181 tggcgcgaaa ataccgtcag catggtattg cgggcggtca tcgtctgctg gttggtgatc
    21241 cagaaggttt tataacccgc ctgtttcatc atgttcatca gcgacggctg cgtcagatac
    21301 agatccgggt tcttttcatt ggcaaaggtc agcgcctgtt gcaggatttc aatggtgtac
    21361 ggacgagacg taactacgtt attaaacacg gtcagattcg gatcggtttt atgcagcgca
    21421 tccagctccg gcgtggtttc acgcggataa ccgtacagac tcatgcgtcc gcgctgggtc
    21481 gactcgccaa tcaccagcac taaagtgcgc ggttcgttac ccgattcatc tttgaaatta
    21541 gccagcggcg gcaaggcatt attttcattc agtagctttg ttagcgagtt tagttgctga
    21601 cgatactgat aatagccggt caggaattgc cacggtgcgg caggctccat acgcgaggcc
    21661 aggttatcca acgttttctc aaacggcttg tttttgataa acgtattcat ggcgatcgga
    21721 tgcagaatca agccataaag cagggcaaaa gagacaatat aacgccacgg ctttggaata
    21781 tagaccgggc gcagacgtgt ccacagcaga actgccattg ccgtataggc cagcgcgata
    21841 agcacaattt tcaggctgaa atactggctt aaatactcgc tggcttcgtt ggtgttggtt
    21901 tcgaacatca caaacagaac gctctgcgag aactcctgcc cgtagatgac gtagtagcat
    21961 agcgccgcca gagaggccgc ccatagcacc acgccgatta ctgcggcaat aattttaatc
    22021 cgcttcggaa agaggaatac cgggatcaac cacagcgagc tgaataacag cgagtcgcga
    22081 atgccgttgg tgccactata accactgatg taaataatgg cctgtagcag agtagagaaa
    22141 aaccaaaagt agagcagtgc ccaacccagg gctttccagc taaaaagggg tttagcctgg
    22201 acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa atatcgccga atgtaacgac
    22261 aattccttaa ggatatctga aggtatattc agaatttgaa taaaatgcag acagaaatat
    22321 attgaaaacg agggtgttag aacagaagta tttcagaaaa ccctcgcgca aaagcacgag
    22381 ggtttgcaga agaggaagat tagccggtat tacgcatacc tgccgcaatc ccggcaatag
    22441 tgaccattaa cgcttgttcg acgcgaggat ccggttcctg gccttctttt tctgcctggc
    22501 gggagcggtg cagtaactcg gcctgcaata cgttcagcgg gtcggtgtaa atattccgta
    22561 gctgaataga ctctgcaatc cacggcagat cggccattag atgggaatcg ttggcaatcg
    22621 ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa ctctttacct aacggccaca
    22681 gtgctttgtc taccaggcgt tggtcatagt attccgccag ccacaggtct gctttggcga
    22741 agaccatctc cagcatgccg agacgcgtcg agaagaatgg ccaatcgcgg cacatggctt
    22801 ccagctcgct ctgtttgcca tcttcgacca ctttttgcag cgccgtacct gcacccagcc
    22861 aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat ccacggtatg gcgcgcagcg
    22921 actcgacgcc gccggttggg cgacgtttcg ccggacgtga acccaacggc agtttgccca
    22981 attcttgttc cggcgtagcg gagcggaagt aaggcacaaa atctttgttt tcacgtacgt
    23041 agccacggta gagatcgcag gagatgactg acagttcatc cataatgcga cgccagctct
    23101 ctttcggctc aggtggtggc agcaggttgg cttccagaat cgccccggtg tacagcgaca
    23161 ggctgctgac ggtgatttct ggcagaccat atttaaagcg gatcatctcg ccctgttcag
    23221 tcacgcgcag gccacctttc aggcttcctg gcggttgtga cagcagcgcc gcatgagcag
    23281 gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa caacgtcagc tcaatacccg
    23341 ctttttcgca ggttttgatt aatgcatcct gtgcctgata ttgcgcccag gaagctgcca
    23401 tcactcccgc atcttttgct gagtcggaat agccaatcat caccatctgt ttgccctgaa
    23461 tcaggccacg ataccagtca atattgagca gctgggtcat gacatcgttg gcgttgttca
    23521 gatcatcgag ggtttcaaac agcggagcaa ccggcatcgc aaacccgata cccgcttctt
    23581 tcagcagcag gtggacagcc agtacgtcgg acggcgtttt cgccatcgag atcacgtagg
    23641 cggcaatgga gccttgcggt gcttcggcaa tcacctggca ggtatcgagc acttcgcgcg
    23701 tttcggcgct tggttgccag ttgcgcggca gaagcggacg tttggagttc agttcgcgga
    23761 tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta gtcgccgata ccgaggtagc
    23821 gggtcagctc gcccagcgct tcggtatgac gcgtgctctc ctgacggata tcaatacgga
    23881 ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc gagcagatcg ccgttggcga
    23941 taatacccat gccacacgcc tgaagtgact ggtagcaagc gtagagcggt tcccacagtt
    24001 cttcgttttg tgtcagcagc ccttctggtt ttggcagttc ttcgcctttc aggcgcgctt
    24061 ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt tttcatcaga tagcgatacg
    24121 gttctgcggc accttcttcg ccaaccagcg ccagcagttc aggggtcgct tcaaccatcg
    24181 acagttcaga aaccagcacc tgaatatctt tcaggaacaa atcggtggct ttccagcggc
    24241 tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt cgggttgccg tcgcggtcgc
    24301 cgcccatcca cgaagtaaaa cggaccggaa caaattcgac gggcagtttg tagccgaggt
    24361 tctcttccag ttgttcgttc agttcgcgca ggtaatttgg tacgccttgc cacaggctgt
    24421 tttccactac ggcaaagccc catttggctt catctaccgg gcttggacgc agcttacgga
    24481 tttcatcggt atgccatgac tgggcgatta actggcgcag gcgacgcatc agctggttgt
    24541 gttcgtagtc agcgatatct ttgttatcga gctgttttaa gcaggcgttc acttccacca
    24601 ttttgtggat cagtgtacga cgggtaattt cggtagggtg agccgtgagg accagttcca
    24661 gcgacagcga ttccactgct tttttgatgg tgtcttcgct cagttccggc tggtttttca
    24721 gtttacgcag ggtgcgggcg atcacttccg ggttgctggc agcttcgcct ttcggcgaaa
    24781 tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa ctgactaaac gcacgcgcaa
    24841 cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt gagcaactcc tggcggttag
    24901 catcattgcc agcgcgtgaa gatttcgaca acttacggat agtttctacg cgttcaagaa
    24961 tgtgttctcc caacgcatcc ttgatggttt ctcccagcac tttgccgagc atactgacat
    25021 tactacgcaa tgcggaatat tgttcgttca tattacccca gacaccccat cttatcgttt
    25081 gatagccctg tatccttcac gtcgcattgg cgcgaatatg ctcgggcttt gcttttcgtc
    25141 gtcttttata aagccacgta aaagcggtga cgtcaaatgc tgcgaaatcg cttcagcaaa
    25201 cgaataaata gcaggaattt acgtcattaa attcacgacg ctttaaataa gcgtaactta
    25261 tggaaatgtt aaaaaatcgc cccaagtaac accaaaggtg taggtcggat aagatgcgca
    25321 agtatcgcat ccgacattat tgcggcactg gagtttgaca acagtgccgg atgcggcgcg
    25381 agcgccttat ccggcctacg ttttaatgcc agcaaaaatg gtgaattacc tgggttatca
    25441 gttcgcgggt gggcttgata aaccgtgttt ccagatattc atcaggttga tgagcctgat
    25501 taattgagcc aggccccaac accagcgtcg ggcataacgt ttgaataaac ggcgcttcgg
    25561 tacagtagtt caccacttcg gtctttgctc cgagcaattt ctcaactact tcaaccagtt
    25621 gatgattcgg tgggcattca tagccaggga tcggcggatg cagctcgtcg accgtcagac
    25681 gacccggcca gcgttcgctc accggagcca atgcatcgtt gagcaaacca ttaagttcat
    25741 tgagtgtcat gccaggcagc ggacgaatat ccatatgcaa ctcacagcaa gcgcaaatac
    25801 ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag cgtagggtat ggcacggtaa
    25861 acgcttcgta gtgataacgt tctttcaggt tatcgcgcaa ttgcaaaata tgcccgatgg
    25921 cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg atcgctggag tgccccgact
    25981 ggccctgaat acggatggcg ttagagatat gacctttatg tgcgcgtacc ggttgtagtg
    26041 acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc ggtagtttcg gcaaaataac
    26101 gcgctccggc catactggtt tcttcatcag cagtcgccag aatgtagagc ggttttttca
    26161 gtttcgtgac gtcgacatcg cgtagcgcat caaggataaa cgcaaaaaag cctttcatgt
    26221 cggcggtgcc taagccgtaa agcttgccgt catgctccgt cagtgtaaac ggatcacgcg
    26281 tccagcgacc gtcatcaaat ggtaccgtat cggtatgccc cgccagcaac aagccgccag
    26341 ccccctgtcc gatacttgcc agcatattaa atttgttgcg agttcctggc acaggctgca
    26401 cttccacatt gaagcctaaa tctttaaacc agtccgccag cagagtgatt aaatctgcat
    26461 tgctttgatc gagtgcctct tccgtggcgc ttattgaagg tgtggcaatc agagcacggt
    26521 aaatctcgat aaatggcggt aatttgtttt tcattgttga cacacctctg gtcatgatag
    26581 tatcaatatt catgcagtat ttatgaataa aaatacacta acgttgagcg taataaaacc
    26641 caccagccgt aaggtgaatg ttttacgttt aacctggcaa ccagacataa gaaggtgaat
    26701 agccccgatg ttgaatacgc tgattgtggg tgccagcggc tacgctggcg cagagctagt
    26761 gacctatgta aatcgccatc cgcatatgaa cataaccgct ttgactgttt cagcgcaaag
    26821 caatgatgcg ggaaagttaa tctccgattt gcatccgcag ctaaaaggca tcgttgatct
    26881 gccgttgcag ccgatgtcgg atatcagcga gtttagccca ggggtggacg tagtgtttct
    26941 cgccaccgcc catgaagtta gccacgattt agcgccgcag tttcttgaag cgggctgcgt
    27001 ggtgttcgac ctttccggcg cgtttcgtgt taacgacgcc accttctatg aaaaatatta
    27061 cggctttacc catcaatacc cggaactgtt ggaacaggca gcctacggtc tggcggagtg
    27121 gtgcggtaat aaattaaaag aagcgaattt gattgcggtg ccgggctgtt atccgacggc
    27181 ggcacagctg gcgctgaaac cgttgattga tgccgatctt cttgacctca atcagtggcc
    27241 ggtgatcaac gccaccagcg gcgtgagcgg tgcagggcgt aaagcggcca tttcaaacag
    27301 cttttgtgaa gttagcctgc aaccgtatgg cgtctttact catcgccatc aaccagagat
    27361 cgccacacac ctcggtgctg acgttatctt caccccacat ctgggcaatt tcccgcgcgg
    27421 cattctcgaa accattacct gccgcctgaa atcgggtgtg acccaagcgc aagtcgcgca
    27481 agtgttacag caggcgtatg cccataaacc gctggtgcgg ctgtatgaca aaggcgttcc
    27541 ggcgctgaaa aatgtcgttg ggctgccatt ttgcgatatc gggtttgccg ttcagggcga
    27601 gcatctgatt attgtggcga ccgaagacaa cttactgaaa ggcgcggcgg cacaagcggt
    27661 acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag tctcttattt aagggtgcaa
    27721 tgatgaatcc attaattatc aaactgggcg gcgtactgct ggatagtgaa gaggcgctgg
    27781 aacgtctgtt tagcgcactg gtgaattatc gtgagtcaca tcagcgtccg ctggtgattg
    27841 tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg gctgaatctg ccggtgaaaa
    27901 agaaaaacgg cctgcgggtg acgcctgctg atcagataga cattatcacc ggagcactgg
    27961 cgggaacggc aaataaaacc ctgttggcat gggcgaagaa acatcagatt gcggccgtag
    28021 gtttgtttct cggtgacggc gacagcgtca aagtgaccca gcttgatgaa gagttaggtc
    28081 atgttggact ggcgcagcca ggttcgccta agcttatcaa ctccttgctg gagaacggtt
    28141 atctgccggt ggtcagctcc attggcgtaa cagacgaagg gcaactgatg aacgtcaatg
    28201 ccgaccaggc ggcaacggcg ctggcggcaa cgctgggcgc ggatctgatt ttgctctccg
    28261 acgtcagcgg cattctcgac ggcaaagggc aacgcattgc cgaaatgacc gccgcgaaag
    28321 cagaacaact gattgagcag ggcattatta ctgacggcat gatagtgaaa gtgaacgcgg
    28381 cgctggatgc ggcccgcacg ctgggccgtc cggtagatat cgcctcctgg cgtcatgcgg
    28441 agcagcttcc ggcactgttt aacggtatgc cgatgggtac gcggatttta gcttaagttt
    28501 tgttggccgg aggcgcagct ttccggcatt gaatttcaaa ataaggaaac agagttatgg
    28561 cactttgggg cgggcgtttt acccaggcag cagatcaacg gttcaaacaa ttcaacgact
    28621 cactgcgctt tgattaccgt ctggcggagc aggatattgt tggctctgtg gcctggtcca
    28681 aagccctggt cacggtaggc gtgttaaccg cagaagagca ggcgcaactg gaagaggcgc
    28741 tgaacgtgct gctggaagat gttcgcgcca ggccacaaca aatccttgaa agcgacgccg
    28801 aagatatcca tagctgggtg gaaggcaaac tgatcgacaa agtgggccag ttaggcaaaa
    28861 agctgcatac cgggcgtagt cgtaacgatc aggtagcgac tgacctgaaa ctgtggtgca
    28921 aagataccgt tagcgagtta ctgacggcta accgacagct gcaatcggcg ctggtggaaa
    28981 ccgcacaaaa caatcaggac gcggtcatgc caggttacac tcacctgcaa cgcgcccagc
    29041 cggtgacgtt cgcgcactgg tgcctggcct atgttgagat gctggcgcgt gatgaaagcc
    29101 gtttgcagga tgcgcttaag cgtctggatg tcagcccatt aggctgtggc gcgctggcgg
    29161 gaacggccta tgaaatcgac cgtgaacagt tagcaggctg gctgggcttt gcttcggcga
    29221 cccgtaacag tctggacagc gtttctgacc gtgaccatgt gttggaactg ctgtcggctg
    29281 ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga tctgattttc tttaacaccg
    29341 gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc cggttcatca ttaatgccgc
    29401 agaagaaaaa cccggatgcg ctggagttga ttcgcggtaa atgcggtcgg gtgcaggggg
    29461 cgttaaccgg catgatgatg acgctgaaag gtttgccgct ggcttacaac aaagatatgc
    29521 aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg gctggactgc ctgcatatgg
    29581 cggcgctggt gctggacggc attcaggtga aacgtccacg ttgccaggaa gcggctcagc
    29641 agggttacgc caacgccacc gaactggcgg attatctggt ggcgaaaggc gtaccgttcc
    29701 gcgaggcgca ccatattgtt ggtgaagcgg tggtggaagc cattcgtcag ggcaaaccgc
    29761 tggaagatct gccgctcgac gagttgcaga aattcagccc ggtgattgac gaagatgtct
    29821 atccgattct gtcgctgcaa tcgtgcctcg acaagcgtgc ggcaaaaggc ggcgtctcac
    29881 cgcagcaggt ggcgcaggca attgcttttg cgcaggcgcg gttggggtaa gaacatttat
    29941 atgtataaat ttgagcctgg cttatcgccg ggctttttta tggcaaaaaa agcggatcct
    30001 ggagatccgc aaaagttcac gttggcttta gttattcgag ttgagaaact ctcgaaacgg
    30061 gcagtgactt caagggttaa aagaggtgcc gctccgttcc tgtgagcaat tatcagtcag
    30121 aatgcttgat agggataatc gttcattgct attcttccta tcgccatgaa ctatcgtggc
    30181 gatggaggat ggataatgaa tattcgtgat cttgagtacc tggtggcatt ggctgaacac
    30241 cgccattttc ggcgtgcggc agactcctgc cacgttagcc agccgacgct tagcgggcaa
    30301 attcgtaagc tggaagatga gctgggcgtg atgttgctgg agcggaccag ccgtaaagtg
    30361 ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc gtaccgtgct gcgtgaggtg
    30421 aaagtcctta aagagatggc aagccagcag ggtgagacca tgtccgggcc gctgcatatt
    30481 ggtttgattc ccacggtcgg gccgtacctg ctaccgcata ttatcccgat gctgcaccag
    30541 acctttccaa agctggaaat gtatctgcat gaagcacaga cccaccagtt actggcgcaa
    30601 ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg tgaaagagag cgaagcattc
    30661 attgaagtgc cgttgtttga tgagccaatg ttgctggcta tctatgaaga tcacccgtgg
    30721 gcgaaccgcg aatgcgtacc gatggccgat ctggcagggg aaaaactgct gatgctggaa
    30781 gacggtcact gtttgcgcga tcaggcgatg ggtttctgct ttgaagccgg ggcggatgaa
    30841 gatacacact tccgcgcgac cagcctggaa actctgcgca acatggtggc ggcaggtagc
    30901 gggatcactt tactgcctgc gctggctgtg ccgccggagc gcaaacgcga tggggttgtt
    30961 tatctgccgt gcattaagcc ggaaccacgc cgcactattg gcctggttta tcgtcctggc
    31021 tcaccgctgc gcagccgcta tgagcagctg gcagaggcca tccgagcaag aatggatggc
    31081 catttcgata aagtgttaaa acaggcggtt taaaccgttt aacgcagcta cccgataggc
    31141 ttccgccatg gtcgggtagt taaaggtggt gttgacgaag tactcaatag tgttgccgcc
    31201 acctttctgt tccataatcg cctgaccgat atgaataatt tcggcagcgc gctcgccaaa
    31261 gcagtgaata cccagaatct cttttgtttc ccgatggaac aaaattttca gcgtgcccac
    31321 gttcatgccg acgatttgtg cgcgtgccag atgtttaaac tgggcgcggc ccacttcata
    31381 tggcactttc atcgcggtca gctgctgttc ggttttgccc acagagctga tttccgggat
    31441 ggtgtaaatg ccggtaggga tatcttcaat cagatgcgcg gtggcttcgc cttttaccag
    31501 cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat gccaggctcg gataaccaat
    31561 cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga tacatgctgt tgaccttcag
    31621 ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac gccagcgaat cggtattacc
    31681 ggtacgaccg ttggcataga gcaggcagtc agctttcagt tttttacccg acttcagatg
    31741 catgatcaca ccgtcgtcac agccttcgat cttctcgtac tcttcgttgt gacgaatcac
    31801 tacgccactg ttccagaagt gataggagag agaatctgac atctcttgat cgagaaatgc
    31861 cagcaggcga tcgcgggtgt tgatcagatc cacttttaca tccataccgc ggaagatcga
    31921 cgcatattca cagccgatca ctccagcacc atagataagt acatggcgcg gttcgtggtg
    31981 catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg gtgaaatcaa catctgttgg
    32041 atgatatgga cgagagccgc aggcaataac aaatttttca gcggttagtg tttcaacgct
    32101 gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca aagcgagcgt ttccctgcaa
    32161 tatttcacag tgattacgtt cgtaaaatcc ctgacgcatg cgcgtttgtt gattaatcac
    32221 gttatcggca tggttaagga tatcggcaaa agaagagcgg agcagtcggg aatggtcgct
    32281 gtaaagtggg ttttgattga attctataat gcggctgacg gcgtgacgga gagctttcga
    32341 cgggatggtg ccccagtggg tgcaaccgcc gccaacattt tgataacgct cgataactgc
    32401 gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct tcgccgccgg ggccggaacc
    32461 tattactatg gcatcgtaat cgtaggaatg tggcatggta gggcttacct gttcttatac
    32521 ataaaagcaa cagaatggta acattttatc gcgggtaagc caattgatcc ccgtcattta
    32581 tctggctata tcctgagcgg cctttgcttt gtctgtttct tacttttgcc ctgacgtttt
    32641 attggatttt tatcgacgat actctccgtt taagcggcag gtttccgctg tacgtaaaag
    32701 aaccggccaa agaattgcag taaatatgtt ttattgcgtt accgttcatt cacaatactg
    32761 gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg caaggattca
    32821 gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc gctggtggaa
    32881 gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt gcgtgaagtg
    32941 gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga cgtagacgaa
    33001 ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat gcgccaggcg
    33061 cgtcagcgta tcgccaaagg cgggagtgtg atccgcacct cggtctccac atttatggag
    33121 ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc cggcacctcc
    33181 gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga acttgcggac
    33241 tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc cgaagcaatg
    33301 gtgacaattg tcttcagtgc gggtgccgag gcgttggaca tcggcgtcga acaacgtcgg
    33361 caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc ttattactgg
    33421 tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga cgagtaatga
    33481 aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt agttgctggc ttatcgatta
    33541 atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc ccgattattt
    33601 ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcact atgccggtag
    33661 gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac agcacggtag
    33721 ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc tccgtcatta
    33781 tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct gagtaatcgg
    33841 aatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag taatacgccg
    33901 cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc gaccttgtgc
    33961 gtctggctta atgtttccag attcttgcat aacgtttccg gattgcagga gatatacaga
    34021 atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct gcgcggcggg
    34081 tcgacaaaaa tggtttcgca ctgataactc ttcaagtcga tcccttgcag gcgattaaac
    34141 tcgcgtacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat aatttgtacg
    34201 ttatcaatat ggttagctgc gatgttgtat tgcgcagcag caaccgacgg cttagcgatt
    34261 tcggtggcta atacccgatc aaaattgcgc gccagtgcta atgaaaagtt accgttgccg
    34321 cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc ccattccagc
    34381 atctgaatat tcatcgccgc gttcggctgg gtaaaactgt tttctacctg acggtagatc
    34441 atctctttcc ctgcgaccgg cagacgttca tcgatgtaat cttgatccag ctcgattttg
    34501 gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag tgcatcgcgc
    34561 agggcttccg cttcctgacg ccactcatca tccagctttt tatggtacaa cagggaaacc
    34621 accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg gcgcagaacg
    34681 ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag ttcactggca
    34741 gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat gatgtgatac
    34801 aggtcatcgc catcgtgcca gatgcggaac tccgcacgca tccggtaatg actgaccggc
    34861 gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg caaacgtacc
    34921 actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg ggtcatgatg
    34981 tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat gtccagatct
    35041 tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc tgtagcatcc
    35101 acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctggagctg acgcgcagcg
    35161 gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg aagtcatcat
    35221 ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg tcgcatctgg
    35281 ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt gtggatgctt
    35341 tacaatgatt aaaaaagctt cgctgctgac ggcgtgttct gtcacagcct tttccgcttg
    35401 ggcacaggat accagcccgg atactctcgt cgttactgct aaccgttttg aacagccgcg
    35461 cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc gctggcagtc
    35521 gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc aaaacggcgg
    35581 ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg tgttggtgtt
    35641 aattgatggc gtacgcctga atctggcggg ggtgagtggt tctgccgacc ttagccagtt
    35701 ccctattgcg cttgtccagc gtgttgaata tatccgtggg ccgcgctccg ctgtttatgg
    35761 ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac ccggaacgga
    35821 aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta cgcagcaaca
    35881 actgggggat aagacacggg taacgctgtt gggcgattat gcccatactc atggttatga
    35941 tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg gttttttaag
    36001 taaaacgctt tatggcgcgc tggagcataa ctttactgat gcctggagcg gctttgtgcg
    36061 cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg gttcaccgtt
    36121 gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct ataacggcga
    36181 actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca actacgatcc
    36241 ccattttggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat acaccgtcca
    36301 gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg actggcagaa
    36361 acagactacg acgccgggta caggttatgt tgagaatgga tatgatcaac gtaataccgg
    36421 catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg cagcacgcag
    36481 tgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg gttgggaatt
    36541 catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac caaatctggg
    36601 gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca aacagtggga
    36661 aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat atcgtaacga
    36721 tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg aagggaaagc
    36781 gcggattaag ggcgtcgagg cgaccgccaa ttttgatacc ggaccactga cgcatactgt
    36841 gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt tacgccgtgc
    36901 taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact ggggtattac
    36961 ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt atcaaaccgt
    37021 taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca cctctcacct
    37081 gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag tctatggcta
    37141 ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa ccacgtccca
    37201 ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag atccggcatc
    37261 tcttaccgga tctccattac atttatgctt tcgataacgt cgcttttccg tatggcgaaa
    37321 aaagcgaaga gtttattgtt gagcgagtgg tggcaattgt caccgcagtg caagaacgtt
    37381 atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca cttcctgcat
    37441 tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa cctgctgcac
    37501 gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaactgtt aaacgttctt
    37561 atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg ctgggctcgg
    37621 cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct ctggatgcac
    37681 taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc gttgtattgg
    37741 gttgcaccca tttccctcta ctacaagaag aactcttaca ggtgctgcca gagggaaccc
    37801 ggctggtgga ttctggcgca gcgattgctc gcagaacggc ctggctgtta gaacatgaag
    37861 ctccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg acgccagaag
    37921 ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa aaactggcag
    37981 ttttaggctg atttggttga atgttgcgcg gtcagaaaat tattttaaat ttcctcttgt
    38041 caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg caaacacgcc
    38101 gccgggtcag cggggttctc ctgagaactc cgacagagaa agcaaaaata aatgcttgac
    38161 tctgtagcgg gaaagcgtat tatgcacacc ccgcgccgct gagaaaaagc gaagcgg
//
LOCUS       DAZFRN010000032        37588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFRN010000032 DAZFRN010000000
VERSION     DAZFRN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.032"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..129)
                     /locus_tag="ACHKUB_001552"
     CDS             complement(<1..129)
                     /locus_tag="ACHKUB_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO6654588.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVSFIMQSNVLHRLN"
     gene            complement(452..751)
                     /locus_tag="ACHKUB_001553"
     CDS             complement(452..751)
                     /locus_tag="ACHKUB_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654589.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTAPAIRLALHHEIADFCATLEAPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            complement(748..1014)
                     /locus_tag="ACHKUB_001554"
     CDS             complement(748..1014)
                     /locus_tag="ACHKUB_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO6654590.1"
                     /translation="MTILDYIAANPGCSGSEIATALNTQTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGRASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            complement(1040..1252)
                     /locus_tag="ACHKUB_001555"
     CDS             complement(1040..1252)
                     /locus_tag="ACHKUB_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654591.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            complement(1249..1491)
                     /locus_tag="ACHKUB_001556"
     CDS             complement(1249..1491)
                     /locus_tag="ACHKUB_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654592.1"
                     /translation="MNSIFTATMLTRFTDAVGHEFIVESHLITTTTPCPSDADYLYIH
                     LADGTQITAIASTVREVTAIRGAWKSETQAHGELRP"
     gene            complement(1503..1781)
                     /locus_tag="ACHKUB_001557"
     CDS             complement(1503..1781)
                     /locus_tag="ACHKUB_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654593.1"
                     /translation="MSINTPTEINKLFTDPAHIELTQKTLTEITNVLDERISEIDKDK
                     HFATYMALQMQSMTMVTAKQTISELYMKNLRLERELAELRAQSGQFSA"
     gene            complement(1861..1986)
                     /locus_tag="ACHKUB_001558"
     CDS             complement(1861..1986)
                     /locus_tag="ACHKUB_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654594.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEECRK"
     gene            complement(1983..2225)
                     /locus_tag="ACHKUB_001559"
     CDS             complement(1983..2225)
                     /locus_tag="ACHKUB_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO6654595.1"
                     /translation="MDKEYKVLINKALERFNFRLSMSGVAAEHAARDSLTRAIRSLYD
                     AAFYADDLDALNELSELVRDAECGVHIEPYALGNAA"
     gene            complement(2246..2500)
                     /locus_tag="ACHKUB_001560"
     CDS             complement(2246..2500)
                     /locus_tag="ACHKUB_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654596.1"
                     /translation="MATTTKDMAEIASAYTILIHRLIANNARNALNTAKPLWEAKEDI
                     ISALQGLQKCADLAGDYAAYMAIEDTIEKIESGKSLSDFV"
     gene            complement(2504..2839)
                     /locus_tag="ACHKUB_001561"
     CDS             complement(2504..2839)
                     /locus_tag="ACHKUB_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO6654597.1"
                     /translation="MTQRRNFRSGTERHANRFSTSASRSNSHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPWGN"
     gene            complement(3153..3578)
                     /locus_tag="ACHKUB_001562"
     CDS             complement(3153..3578)
                     /locus_tag="ACHKUB_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO6654598.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGSRNKRISKKAA"
     gene            3686..3985
                     /locus_tag="ACHKUB_001563"
     CDS             3686..3985
                     /locus_tag="ACHKUB_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6654599.1"
                     /translation="MSIPQNEKLKLIRESERMKMKELSDLIGINYYTYHGYESGKSKM
                     PLDFGILLLRHARFRKYRDWFLFDEVEPKAGQVVPALAHIGQSEAESSHSGKKTG"
     gene            4052..5047
                     /locus_tag="ACHKUB_001564"
     CDS             4052..5047
                     /locus_tag="ACHKUB_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6654600.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(5141..5578)
                     /locus_tag="ACHKUB_001565"
                     /pseudo
     CDS             complement(5141..5578)
                     /locus_tag="ACHKUB_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(5560..6666)
                     /gene="bcsZ"
                     /locus_tag="ACHKUB_001566"
     CDS             complement(5560..6666)
                     /gene="bcsZ"
                     /locus_tag="ACHKUB_001566"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO6654601.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(6673..9012)
                     /gene="bcsB"
                     /locus_tag="ACHKUB_001567"
     CDS             complement(6673..9012)
                     /gene="bcsB"
                     /locus_tag="ACHKUB_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO6654602.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(9023..11641)
                     /gene="bcsA"
                     /locus_tag="ACHKUB_001568"
     CDS             complement(9023..11641)
                     /gene="bcsA"
                     /locus_tag="ACHKUB_001568"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO6654603.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIHAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(11638..12390)
                     /gene="bcsQ"
                     /locus_tag="ACHKUB_001569"
     CDS             complement(11638..12390)
                     /gene="bcsQ"
                     /locus_tag="ACHKUB_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO6654604.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(12402..12590)
                     /gene="bcsR"
                     /locus_tag="ACHKUB_001570"
     CDS             complement(12402..12590)
                     /gene="bcsR"
                     /locus_tag="ACHKUB_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO6654605.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            12863..14434
                     /gene="bcsE"
                     /locus_tag="ACHKUB_001571"
     CDS             12863..14434
                     /gene="bcsE"
                     /locus_tag="ACHKUB_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO6654606.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAATAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14431..14622
                     /gene="bcsF"
                     /locus_tag="ACHKUB_001572"
     CDS             14431..14622
                     /gene="bcsF"
                     /locus_tag="ACHKUB_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO6654607.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            14619..16298
                     /gene="bcsG"
                     /locus_tag="ACHKUB_001573"
     CDS             14619..16298
                     /gene="bcsG"
                     /locus_tag="ACHKUB_001573"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO6654608.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(16385..16492)
                     /locus_tag="ACHKUB_001574"
     CDS             complement(16385..16492)
                     /locus_tag="ACHKUB_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6654609.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16868..16975)
                     /locus_tag="ACHKUB_001575"
     CDS             complement(16868..16975)
                     /locus_tag="ACHKUB_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6654610.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17351..17458)
                     /locus_tag="ACHKUB_001576"
     CDS             complement(17351..17458)
                     /locus_tag="ACHKUB_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6654611.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17934..19205
                     /gene="yhjV"
                     /locus_tag="ACHKUB_001577"
     CDS             17934..19205
                     /gene="yhjV"
                     /locus_tag="ACHKUB_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO6654612.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(19235..20239)
                     /gene="dppF"
                     /locus_tag="ACHKUB_001578"
     CDS             complement(19235..20239)
                     /gene="dppF"
                     /locus_tag="ACHKUB_001578"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO6654613.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(20236..21219)
                     /gene="dppD"
                     /locus_tag="ACHKUB_001579"
     CDS             complement(20236..21219)
                     /gene="dppD"
                     /locus_tag="ACHKUB_001579"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO6654614.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(21230..22132)
                     /gene="dppC"
                     /locus_tag="ACHKUB_001580"
     CDS             complement(21230..22132)
                     /gene="dppC"
                     /locus_tag="ACHKUB_001580"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO6654615.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(22142..23161)
                     /gene="dppB"
                     /locus_tag="ACHKUB_001581"
     CDS             complement(22142..23161)
                     /gene="dppB"
                     /locus_tag="ACHKUB_001581"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO6654616.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(23312..24919)
                     /gene="dppA"
                     /locus_tag="ACHKUB_001582"
     CDS             complement(23312..24919)
                     /gene="dppA"
                     /locus_tag="ACHKUB_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO6654617.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(25664..25797)
                     /locus_tag="ACHKUB_001583"
     ncRNA           complement(25664..25797)
                     /ncRNA_class="other"
                     /locus_tag="ACHKUB_001583"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(25832..25908)
                     /locus_tag="ACHKUB_001584"
     tRNA            complement(25832..25908)
                     /locus_tag="ACHKUB_001584"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25872..25874),aa:Pro,seq:cgg)
     gene            complement(26000..27691)
                     /gene="eptB"
                     /locus_tag="ACHKUB_001585"
     CDS             complement(26000..27691)
                     /gene="eptB"
                     /locus_tag="ACHKUB_001585"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO6654618.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            complement(28015..29223)
                     /gene="yhjX"
                     /locus_tag="ACHKUB_001586"
     CDS             complement(28015..29223)
                     /gene="yhjX"
                     /locus_tag="ACHKUB_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6654619.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            complement(29452..30150)
                     /gene="yhjY"
                     /locus_tag="ACHKUB_001587"
     CDS             complement(29452..30150)
                     /gene="yhjY"
                     /locus_tag="ACHKUB_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6654620.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            30308..30871
                     /gene="tag"
                     /locus_tag="ACHKUB_001588"
     CDS             30308..30871
                     /gene="tag"
                     /locus_tag="ACHKUB_001588"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO6654621.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPTSDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            30868..31308
                     /gene="yiaC"
                     /locus_tag="ACHKUB_001589"
     CDS             30868..31308
                     /gene="yiaC"
                     /locus_tag="ACHKUB_001589"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6654622.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPRAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(31277..33610)
                     /gene="bisC"
                     /locus_tag="ACHKUB_001590"
     CDS             complement(31277..33610)
                     /gene="bisC"
                     /locus_tag="ACHKUB_001590"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO6654623.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            33763..34422
                     /gene="yiaD"
                     /locus_tag="ACHKUB_001591"
     CDS             33763..34422
                     /gene="yiaD"
                     /locus_tag="ACHKUB_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO6654624.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            34526..35500
                     /gene="ghrB"
                     /locus_tag="ACHKUB_001592"
     CDS             34526..35500
                     /gene="ghrB"
                     /locus_tag="ACHKUB_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO6654625.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(35550..36260)
                     /gene="yiaF"
                     /locus_tag="ACHKUB_001593"
     CDS             complement(35550..36260)
                     /gene="yiaF"
                     /locus_tag="ACHKUB_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO6654626.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            36694..36984
                     /gene="yiaG"
                     /locus_tag="ACHKUB_001594"
     CDS             36694..36984
                     /gene="yiaG"
                     /locus_tag="ACHKUB_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO6654627.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            37265..37477
                     /gene="cspA"
                     /locus_tag="ACHKUB_001595"
     CDS             37265..37477
                     /gene="cspA"
                     /locus_tag="ACHKUB_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO6654628.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
BASE COUNT         9039 a         9865 c         9469 g         9215 t
ORIGIN      
        1 gttaagtcgg tgtaatacgt ttgactgcat gatgaaggag acagatgcag cccgatggag
       61 tctgcggttg atttcagtcc ggatgtgaag acgctcagcg cgggcgcgct gttgcttgcg
      121 gtttgccatg gtgtggcctc ttgtgtagta agttttgaaa actcaccatc cagaggttgc
      181 aattctgggt ggtgagacgt acagggttgc aaccaccggc tacacaagac cccggcccga
      241 ccgaagtcgg ccctgtacgc cccaccataa ttctgacgcg aaaaagatgt gacgatgcgg
      301 tacgcacaaa aaaaccgctg gcgcggttgt gcgcttgtgt agtcagcggg ggttgcaatc
      361 ccggcacccg ttttatgagg tgcgggaaaa ctgtaacccg accaggcgaa cgctggcaag
      421 cggttttttt gtgtgtgcat gttctggttt cttactggtt cagaaaaaaa tcaaaaacct
      481 tgtcaatgcg ttgcagtagc tcttgctgta ttgcttccgg cgtttccggt tcacctggcg
      541 cctccaacgt cgcgcagaaa tcagcgattt catgatggag cgccaggcga atggcaggag
      601 ccgtggttct ggcgtgctcc agctcatcca gcagtgccag cacagcagac ggcgagagca
      661 ttgcgcgaaa tgccagtaat ttttgaggcg ttgccattcg ttgcagggca aatgccagtt
      721 cgcgtagctt ctggtggttg atggtgctca tgctctggct tccttcagta gctggttaaa
      781 catgtgagta agtggattgc tacacccgaa cggcatcggg tttacgtggt aagaagcccg
      841 gcctcctgtt ttgcgagcgc gaccacctgt gctgcggttt gttctgatga ctaagccacc
      901 gcgccagagt cggcgtaact cagcattaat ggctgtggtc tgggtattca gtgccgtggc
      961 gatttcactt ccgctgcacc ccggattggc tgcgatgtag tccagaatgg tcatcgtgag
     1021 atctcctttg cagttccggt tattcagtcg ggttcagggt gtaatcaatg gcgttttcaa
     1081 tacggcgttt aaggtcccgg tggatctcac ctggtgttac tggtgcgccc ggaccgctca
     1141 gcgttgccgt gaagtcgtcg atttcctcca gtaacagatt gcggagtacg gcgatgtggt
     1201 ggcgtgcgtc tttaccgggt ttacgtgcgt tggtggtgta cttgctgatc atggccgcag
     1261 ctctccgtgg gcttgtgttt ctgatttcca tgcccctctg atggccgtta cttcgcgaac
     1321 ggtactggca atcgcggtga tttgtgttcc gtcagccaga tggatgtaga gataatcagc
     1381 gtctgacggg cacggcgtgg tcgttgtgat caaatgactt tccactatga actcgtggcc
     1441 cacggcatcg gtgaaacgcg tcagcatggt ggcggtaaat atgctgttca tcgtcctgcc
     1501 cctcacgctg aaaactggcc ggactgtgcc cggagttctg ccagttcgcg ctcgaggcgc
     1561 aggtttttca tatagagttc actgatggtc tgttttgctg tcaccattgt catgctttgc
     1621 atttgcaggg ccatgtaggt ggcaaaatgc ttgtctttat ctatttctga tattctttca
     1681 tccagtacat tggttatttc agtcagcgtt ttttgtgtca attcaatatg cgccgggtcg
     1741 gtaaatagtt tgtttatttc tgtaggtgta ttgatgctca tggctgtttc tccgtttctg
     1801 gattgagtga agccctccgc agatgcggat ggttttaatg ttttctgtcc ggttggttat
     1861 ttatttacgg cactcttcgc cagtaaataa agattcgata tttttcagcg cttcgataat
     1921 tcgcataatg ccaattgcca taagcacgga aataatcaga atcacccatg aaagaaatat
     1981 gctcatgctg cattcccaag tgcgtatggc tcaatatgta ctccgcattc tgcatcacga
     2041 acaagctcag acagttcatt gagagcatcc agatcgtcag cgtaaaaagc tgcatcatac
     2101 aggctgcgaa ttgctctggt cagtgagtct cttgctgcat gttctgcggc aactcctgac
     2161 atactcaggc gaaagttaaa gcgctcaagg gctttattga tgagaacctt atattctttg
     2221 tccattgtat ttccccggaa aacaattaaa caaaatcgct taatgattta cctgattcaa
     2281 tcttctctat cgtgtcttca atagccatgt atgcggcgta atcgcctgcg aggtcggcgc
     2341 atttctgtag cccctgtaat gcgctgatga tatcttcttt tgcttcccat aaaggttttg
     2401 ctgtatttag tgcattacgg gcattattag ctatgagccg gtgtatgaga attgtgtagg
     2461 ctgatgctat ctctgccatg tcttttgtcg ttgttgccat gtattaattc ccccatggat
     2521 gctgaaaact gcgaccgctg attaaccggt ctgccgttct taatgcttcg tgcagggtga
     2581 aatcctgccc gaactgctca tctccgcgag tcagggagta acaggtattt ccggtaatcg
     2641 ggttgcgtgc gcctctgtgt atgacaattc cggcatcatt aatcagccag gtgtgtttac
     2701 cggtttgttt aaccagatgc ccgtcaggtg tggcgtgcgt ttcctgcagg ctgtagtgcg
     2761 agttgctgcg tgatgcgctg gttgagaaac ggttagcgtg gcgttctgtg ccgctgcgga
     2821 agttgcgtcg ctgtgtcata tcggaaaaac ccgcttaagt taaaagaatt gaaataacgg
     2881 tgtattggca tttatgccca gttgtctttt cttattactg tttcattctg aaatcctgtc
     2941 tgctccattg cattttttaa ggactcttct gtttcaaatg tcacaacgat gcaatgggtc
     3001 gggtaatgga aatacaaatc gttgccagcc cgaacaatgg catctaagtt ctgattacag
     3061 attattactg aagcgtcgtc gcttattttt attctttgtc tgatttccgt gtgtttaatc
     3121 atcttgcccc tccaatgatt gccagttcta tatcacgcag cttttttact gatgcgttta
     3181 ttccgtgaac cagctgctgg cttaaataaa aaaagtcggt tttcccattc gtgccattct
     3241 tgcggtgcgt tttcagtgag tcgatcgcag gcttcatccc acgctttccg gttgatgtag
     3301 agttcggcgt ttccgccagg cttgagaggg tccgccatgt acacagcggg taatttccct
     3361 gcttttgcca tgctcacgat ggcgtcaacg gtcttgccga tatacatcgc aaacccttct
     3421 tttgacagga gatgagaagg gttttgtgaa agcctgattt cctggcgttc aggggctttg
     3481 cgcccggtct tttggtgtgc ggtttctggc gcatctgtca actgatcgca ctgtgtttcg
     3541 gcaggcgatt caggcgcgtt ttgagtgact tcttgcatct ggtatcctcc gttattggcc
     3601 cctttttcga atagggacca tgtagaacca attgcgacta ttcttgtgtt gctatacaca
     3661 aaaattattg gagattagaa aaattatgtc aataccgcaa aatgagaaat taaaacttat
     3721 cagagagtcc gaacggatga aaatgaagga actatctgat ttaattggaa ttaattacta
     3781 tacatatcat ggctatgaaa gtgggaaatc taagatgcct cttgattttg ggatccttct
     3841 tttgcggcac gctcgtttcc gtaaataccg tgactggttt ttgttcgatg aggtcgaacc
     3901 aaaggccggg caggtggtac cggctctcgc acacattggg caaagcgaag cggaatcatc
     3961 ccactccggc aagaagactg gttaacggtc catctggcct ttatttctca aaattgtgat
     4021 caagatgatg ttttcattgg ggggcttcct tatgtcgatt aagaagctcg ctgatggcaa
     4081 atactgtgtg gacgtgcggc ctgctggatc tgaaggaaga agatttcggc ggcgatttcc
     4141 tacccgtggt gaagcggcga tgtatgaacg ccatgttctg acgcattatc acgataagga
     4201 ctgggtcgaa aaacctattg aacgtaaagc gttaagcgat cttctggaac tgtggtgggt
     4261 gtaccacggt aagaatcatc gttatggcgc gatgaatcgg gtcagaattg aagcggttct
     4321 tcgtgatatg caagagcttg gtattaacag aagtgaccac ttaacccgaa aaaatattat
     4381 cagatatcgc cttgaattaa tgaatcgcgg aattaagcag tcaacggtaa atcgttattg
     4441 tgcaatgatg agcggtttct ttgaaaagct gattgatgtt gaagagtttg tgggtgataa
     4501 cccgtttcac gagatacgca agctaacaat taatcagccg gaaatggctt atcttgcaca
     4561 tgatgatatt cccagactaa ccgcactttt atctggcgat aatcttaagg ctgtattgtt
     4621 aagcctggct acaggcgggc gctggggtga agttgccaat attaaaggtg aacacataat
     4681 tggtgggaag gttattttca tggagacaaa gaatggatcg cggcgggtta ttcctatagc
     4741 taaagatctt gaatctctga ttaaagtcaa agcgacggga agattaatga accccagtta
     4801 tgcgatagtc agaagtgcaa taaggactgt gcggcctgat ttaccagtcg gtcagtctgt
     4861 gcatgtttta cggcacacat tcgcaactca tttcatgata aatggaggga acattattac
     4921 ccttcagcgt attttagggc acagcacaat tcagcagaca atgacctatg cgcactttgc
     4981 acctgattat ctccaggacg cggtaaggtt caatccggtt gcggagttgt ccagattttg
     5041 cccataaacg gggactaaat ggcactattc cgaactactt tgaactcgta accatttgat
     5101 atataaaggg ggatagcgat acgcactacc cccttatgcc accttcgggc ggcgcaccgt
     5161 tgaacagttt gttgtaactc gccacagctt cttctgcatg accggtcgtc gcctgcaatc
     5221 gtgcctgttg cagtgcctga cgaccatccg gcgtggaaag tagcatcgta gtccgcgacg
     5281 atttatacgc attcgaactc ggcgctaact gcgacagccg atcgagctgt ttttgcgcgc
     5341 catcaatatc gccctgacgt aacaaagaac ggaaacgggc ggcaacgacg tccgggttat
     5401 tcggatcaat aagttccagc cgatataacg actgttgcac cagatcttca cgatgggtcg
     5461 cttcgcctaa ccgaacttgc tccagcaact gttgctgagc ggttggtgct gcctcgacca
     5521 tcggcatgac ggccagaccg agggaaagcg tgaatatgtt tagtgtgaat ttgcgcattc
     5581 ctggccccag tcaggtaata actcaccttt tgtcgagaag cggaaacggt gttgatccca
     5641 gccttgtcca aacagggtca gcacatagtt gtaataggca tcgctgccgg gaaagttatc
     5701 ggccacgcgc tggcgctgaa cggcctgcgc atcgcggttt tgtaaaaagg gcagcatggc
     5761 ggcagaaaaa ccgaccggtc ctttaccctg cgctttcccc gtagccacat ccactttttc
     5821 cggcggataa ccgtttttct cagtgaatgt cgccatcggt ttaaaccggt tgagcatccg
     5881 cgctttttgc ggatcgctgt caggcatcat gcctacccac atgtaaacgc ggatagcgtc
     5941 gtagctgctg atcaatgttt tttcggcttt tagctgccag cctttgtctt tctcatagcg
     6001 cacccagtct ggcgaaaaac ctttcggggc ggtttccagc aataaacgtt gattggtttc
     6061 gcgcagcgtg gtccacggcg cgccaaagcg ggtgaaatac tgcgccagcg tcggcggcag
     6121 gtagctgggg ttaaaacgcc agctgttatc ctcagcaaaa cccactttgc ccggtaacaa
     6181 catggaaccc agcccaggca ccgtcaccac ttcctcccgc gcgatacgtt ttagcaacgc
     6241 gctgccgatg tcggtataac gctgctcttt ccacaaacgc cccgcctcca gcaacgacca
     6301 ggccatccag acatcaccat cggaggccga attgctgtcc agcacttccc acttactgtt
     6361 ctctttcttg ccccacagcc aggcgggcaa acgttctttt aaagagccct gagcgagatt
     6421 gttctgcgtc cagtcgagaa tattatcgaa agctacacgg tcgttagccg ccagggcaaa
     6481 gaacataccg taactttgcc cttcggaggt ggtgattttg cgcgcgtcgc tggggtcgat
     6541 gacgcgccct tcctgactga tgtaatcctt tttaaactgc tcccaggcag gccaggtaca
     6601 ggctgcctga acactaaagg cagccaacag cagcatcgtc acgattccac tacgcaacac
     6661 attcatcttc aattactcgt tatccgggtt aagacgacga cgactaataa ttcgcagcag
     6721 acgccacagt acccatgcca gcaatatcac actgatagcc gccagcaccg ccagcagaat
     6781 cggatggttt gccagcgcat accacaagcg ctcgaaccac ggcagatgac ctacgtaata
     6841 aacgtcgcca acacgtaggc tgttgatacc ggactcgcgg atcaccgcga ccgaaccgaa
     6901 catggtggcg cgtttgccgc tatcgttcac cgcatcgtta agcatttcat aaccgcgtgg
     6961 gctatctgcc agcagcgcaa tcacgctgcg ctggtcgtta tacggcgact ggaagccaat
     7021 caccgccgcc atcgcaccgg aagaggtcag cgttgactgg gtttctgccg cgcgatcgct
     7081 ctcgtccggc acaatgccgg ggaacggggt ctggcgcatc ggtgttttca cccagctttc
     7141 ggtcgcctgc accaataggt cgatctgctt atcgtctttc agtttgtccg ggataccacc
     7201 gatgatcatg atgtcggcat ctttgccctg aatggtgctg ccatcatcgg tcaccgtcag
     7261 attaatcgcc gggaagcccg tctgtgcgcc gataaaacca acagtattca gcaacgtttc
     7321 catctgtgct tcgttaggcg ctttcggcat cacggtgatg gtttgcgaca gatccgccat
     7381 ccggctgaat gggaagcccg cgttagcaaa ggcgcgtaga tccggcatcg ggatgaagtg
     7441 gtaatacttc gagaagtcga tggtggagtc gtcaccaatc accacatgat tctgcaccgg
     7501 ctggaaggta atacagttat ccaccgaacc gcccggcatc gggttcatat actcaaagtc
     7561 gaagcgcagc tggttggtcg cgcccagttt cagcgccgga atagagacat ctgttttgcc
     7621 atccagcaaa ccttgtaata ccggaatccg cagcagcagg cggttcgcct cctgtttgct
     7681 gctcaggttg aaggattgca ggaactggtt attcaggctg atatccatcc gcgaactgtc
     7741 tttcaccggc ggcatggtgt agcggtaatt aatatccata tcaatgccgg tactgcgcat
     7801 caggtagaga tccggcggca ggtttagcga aacgttaatc gctgctggct caagaccgct
     7861 ggattgtaac tgttcttcat aggttttcag ttcgccaaat gtgaccggac gatcggtacg
     7921 tacccagttc ggcgcatcgt acggcttacg cggtagcagc ggtttcactt cattcactac
     7981 cacgctttca ccacggaaca gaatgttacc ctgagcgata cctttcgctg cctgcaacag
     8041 gtctttgtcg tcacgaccaa acaccaccag cagtttgacg taaggattct gcggatggtt
     8101 aatcatctca atcaccgggg cttttaccgc cggatgatcg cgcaggaagt ccggccgttt
     8161 gtcgttggtt gcaaagacaa tggcattacg atccggcagt tggttataga gcaccgggaa
     8221 gttctgccca cgccagccag aacgcgaacc aaaccacgag gcgacaatgg cagaggcttg
     8281 ttgcagccca acatccggcg cacccgcaaa gaccatcggc aaggtgttgg tgcggttatc
     8341 acgcggatca aagaatggca ccgggaagtg tgacaggtca ttcttcacat tcagggtctg
     8401 ataggtcaga tccagtccac tgctccgccc aacatccagc caaagcgtgg tgctggccgg
     8461 gttttcgcac acgtcctgat aatggccgac aaactccagc cgcacacggt tgaagtcggt
     8521 aataaacagt gggttaatgg gcatttgcgc cagcgttttt ttacccaact gttctttggt
     8581 cactggcagc acgcccatca gctcatcatt gagataaacc tttaactgcg actggacagg
     8641 cagtaacgat ggcgatggcg tgtattcgag gttgagcatc gccttcgtca ccacttcatc
     8701 gctgcgcata ccaaactcaa tgctgccgtt cgggttaatg ccacgtagca ccatgctgcc
     8761 cggcggcggc gcaatttgcg caaaggtcag cttcacatca cgcgaaggac cgttctgcgc
     8821 cacgactggc gcatcagcgc cctgcacgcc cggcatcact tgccccacct gcggattttg
     8881 ttccgtctgg gcggctacag ctggctcagc attgatcagt ggttgcgttg ctggcgtcgc
     8941 ctgcgtcatg aaagagggga acgcactcat ccccatagcc actgcacaaa tccagaatag
     9001 ttttcttttc atcgcgttat catcattgtt gagccaaagc ctgatccgat ggttgtgccg
     9061 tttcgctccg ctccgggcgg cgcggaataa acgatacaac ccaggaaacc agagaagtca
     9121 gcacacggaa tatgcccttc accgaagaag gcgcaaactc cgccagatgg cggtagccac
     9181 ggaagccgag cttcagaata tccagcagac tttccagcgg cttatcttcc gggtagctgt
     9241 cctgccagag cgcccatgta tccgcacggg caaacgtaca ctgcacaaaa tcgatatgtt
     9301 gctgggtggt gagcggcatt aatttcagcc caacttcatt acccatcacg cgcgccacct
     9361 gggtcgggaa gacgtattcc tgctgaccgc gtttaagcaa cagattcact ttctgccctt
     9421 ccagaatctg cgcctgaccg ttgatcttga tccccaaacc accgtcggag aaatcctgaa
     9481 cggtacacga gaagaggtga ccatcttcgc gggcaattgc cgcgggcatc gtcatctcca
     9541 cgcggtgcga tcggcgtacc tgtttgcttt ctaccgatac cgcaactgcg ccgccaagaa
     9601 caatcaggtt gtagaacacc cacaccatac tgacgaccac ggtgagcatc tcggttggcg
     9661 ggccatagaa gtagcgccag atgcctaccg caacgcccac caggttgagt agaacaagga
     9721 agatgtaggg ccgcgagatc acccagtcga cgtactcttc ttccaccagt ccacctttgg
     9781 cggtgacgtt aaatttgcct ttgtgcgggt taatcagcgc caccagcgtc ggtggtgcga
     9841 tataccacgc cagcaccgtt tcgtagattt cactccagaa agagtggcga tatttgccct
     9901 ggatcttcga gttggtcagg ctggcatgga tcatatgcgg cagcacgaat agggcgatca
     9961 tcaacgctgg cgcatagatg atgtaggcat gaagcagcag gaacgccagc ggcgcagtca
    10021 ggaagatcag ccgtggaatg cccgacaaga agtggaacat ggcgttgacg tagcacagcc
    10081 gctgagcaaa cttcagtcct ttaccggtga gcgggttatc gagacggaag atttgcacca
    10141 tcccgcgcgc ccagcgaata cgctgaccga tatgcgccga cagactttcg gtcgccagcc
    10201 ccgccgcctg cggaatacgc atatacgcgg aggtatatcc acgacggtgc aaacgcagag
    10261 aagtatgcgc atcttcagtc acagtttcga cagcaatgcc gccaatttca tccagcggct
    10321 tacggcgaat caccgcacag gaaccgcaga agaaagtggc gtcccacata tcgttgccat
    10381 cctgcaccag accatagaac agcgtgcctt cgttcggtgt tttacgaaaa cgccccaggt
    10441 tgcgttcaaa cgggtctggc gagaagaagt ggtgtggagt ctgcatcatc gccagctgtt
    10501 tctctttcag gaaccagccc atggtcattt gcaggaacga tcgcgttggt acgtggtcgc
    10561 agtcgaaaat cgacacgaac tcgcctttgg catatttcag cgcattgttg atgttgcccg
    10621 ctttcgcatg ttcatgagtg gtacgggcga tatatttcac ccccacgttt tgcgcaaact
    10681 ggcgaaactc ttccctaccg ccgtcatcga ggatccagat gttcagcttg tctttcggcc
    10741 agtcgatacc cagcgaggcg taaatagtat ttttcaccac gttgagatct tcgttgtaag
    10801 tcgggacaaa gatatccacc gacggccaca gcgacatatc tttcggcaat ggcaccggct
    10861 gacgattcag cggccatact acctggaagt agccgagcac cagcacaatc cacgcgtacg
    10921 tttcagcgaa gagcagaata agcccgcaca ccaggctgac cggatcgtcc cagttcagcg
    10981 tagaggtgta acgccaccag atataacggc aagaaacggt cagcgacagc acaatcaaca
    11041 ttagcgccga gaagcgcccc ggcatccgcc gtacgatcag cgctacaccc cacagcagca
    11101 tcaggaagat aaactgcgcc agcgggttaa acggttgagt aacgcagatt aacgccagaa
    11161 tcagcgagaa ggtgacgatg ataccgagga tcaaccgccg cgccccggca ctcaaatgac
    11221 cgagctcttt tttctcatca agatgctgtg ttttatggct aacgcgctca ggcagctcgt
    11281 tcatccattg atggtaacgt ccacgaatat tctgcagacc tgaaaatgcc cgcctgcgcg
    11341 gtttcggcgt ttctttgcgc gatgtaccga tcagtaacca gcatgtttga atgagataac
    11401 ggaccgggtc cagcggacgc ggacgcgagg cgttgatatg cggatacagg tttttatgtt
    11461 ctgcgtgaat acgctgccag cgcgggtgct ccagcggaat aaaaatccag gccaggatca
    11521 tccagaaaca gccgagcgtc gcgctgaaag ccgacgcacc gtgacgacga taatcgcgat
    11581 aacgcccgat aagccgcgcg ttgaccggcg ggataagcaa ccaccgggtc aggatactca
    11641 tgatgcactc ccgactggcg ttttcagccc ggagtagttc aacaggcacc agttcgccag
    11701 cgtcagtatc tcttcagccg ccagcgcatc actgcgatat tcacctactg gttgcttagc
    11761 cgccaggcat tcagccatcg cttcatcacg atgaatgagc atcggcagta atcggcgctg
    11821 gctttgcaac caaagctggt aaatatcgtc ctgaacctga ctgccaatac ggaagtcatt
    11881 aatcaaaata tgtgcgccat ccggcagcgc ttgctgatgc agtcggatat ggcagttggc
    11941 atccacattg acgattgcca gcgagtgatc gcacaaactc agcagctggt gggttatctg
    12001 cgaggcatca cgcggtaagt cgattaaaat ccactggtaa cgcccgctgg cttttagttg
    12061 ctgtaagccg gagcaaatat cgctcagccg ggtttgccag tgctgtggat tttcttgttc
    12121 ttcaatggat aactgaccaa aaggcagcaa atcgagctgc gaggtgtagc gcaacccagc
    12181 gtcacgccag tcctggccat ccagcatcgc tctggcccag ccctgacggt gggtaaaatc
    12241 aacattaaat gacaggcgca acaagttgtc cgggcaggca tcgaccacca gaacattttc
    12301 tcccaacatt tgtaatgacc aggctaatgc ggcggtgatg gttgttgtcc ccacgcctcc
    12361 ccgcaccccc tgcaatccca gtacggccat tcaccaatcc cctacttttg ttgcgcaaac
    12421 tctgccagca acggccagcg ttttaatgcc gcggccaact gttcgcgttg ggaaatatcg
    12481 gcataatcaa tatcaggcag tgaaaatgcc tgctttaacg ccacaatatc attctggaag
    12541 atatagccta tcgcgggatc aggcagagta tctggttcat tgttattcat tttgcttgat
    12601 ccctatgaaa taaccgtacc gttcatgaga caattctaac cgtgagcatt tcgccggttt
    12661 attgtttacg gcgagacggg aatttgctca ctttttgact ttccgttacg caacagaaaa
    12721 agttaaaaac gaaaagacta aaatcacacg tcttgtgacc ttctaaattg attcacatgc
    12781 taaatctgat aagttttaat ttcaatggta ggtttatttc ttagctttcg ctagtaaact
    12841 gataatcaga caaaatggtg acatgaggga cattgtggac cctgtattct ctatcggtat
    12901 ctcatcatta tgggatgagc tgcgacatat gccagcaggc ggcgtctggt ggtttaacgt
    12961 cgatcgccat gaagatgcta tcagtctggc gaatcaaaca attgcatccc aggctgcaac
    13021 cgcacacgtc gcggtcatta gcatggacag cgatccggcg aaaatctttc aattagatga
    13081 ttctcaaggg ccggaaaaaa taaaattatt ttcaatgcta aatcatgaaa aaggtctata
    13141 ctatttgacc cgtgatttgc agtgttctat tgatccccat aattaccttt ttattcttgt
    13201 ttgcgcaaat aacgcatggc aaaacattcc tgccgagcgg cttcgctcat ggttggataa
    13261 aatgaataaa tggagcaggt taaaccattg ttcgcttttg gtaattaatc ccggaaataa
    13321 taacgataaa caattttcat tgttgcttga ggaataccgt tcactttttg gtcttgccag
    13381 tttgcgtttt cagggtgacc aacatttgct ggatattgcc ttctggtgca acgaaaaagg
    13441 ggtcagcgcc cgtcagcagc ttagcgttca gcaacaaaat ggtatctgga cattagttca
    13501 aagcgaagag gcggagatcc aaccacgcag cgacgaaaaa cgcattctga gtaatgttgc
    13561 tgtactggaa ggtgcgccgc cgctatcgga acactggcaa ctgttcaaca ataacgaagt
    13621 cctgttcaat gaagcccgta ccgctcaggc ggcgacggtg gtcttttctt tacagcaaaa
    13681 tgcgcaaatc gagccactgg cccgcagcat tcataccctg cgtcgccagc gcggtagtgc
    13741 gatgaaaatc ctcgtgcggg aaaataccgc tagcctgcgc gccaccgatg aacgtttgtt
    13801 attggcctgc ggtgcaaata tggttattcc gtggaatgcg ccactctccc gttgtctgac
    13861 gatgatcgaa agcgtgcaag ggcagaagtt tagtcgctat gtgccggaag atatcactac
    13921 cttgctgtca atgacccagc cgctcaaact gcgtggtttc cagaagtggg atgtgttctg
    13981 taatgccgtc aacaacatga tgaataaccc tctcttgcct gcccacggta aaggcgttct
    14041 ggttgcccta cgtccggtac cgggtatccg cgttgaacaa gccctgacgc tgtgtcgccc
    14101 taaccgtacc ggcgatatca tgaccattgg cggtaatcgg ctggtgctgt ttctctcatt
    14161 ctgtcggatt aacgatctgg ataccgcgtt gaatcatatt ttcccattgc ctactggcga
    14221 cattttctca aaccgtatgg tctggtttga agatgatcaa atcagtgccg agctggtgca
    14281 gatgcgcttg cttgccccag aacaatgggg catgccgctg cctttaacgc aaagttctaa
    14341 accggtcatc aatgccgagc acgatggtcg ccactggcga cgaataccag aacccatgcg
    14401 actgttagat gatgctgtgg agcgctcatc atgatgacca tcagcgatat cattgaaatt
    14461 attgtcgttt gcgcactgat atttttcccg ctgggctatc tggcgcggca ctctttgcga
    14521 cgcattcgcg acaccttacg tttgttcttt gctaaacctc gttatgttaa accggccggg
    14581 acgttacgcc gcacggaaaa agccagggca accaaaaaat gactcaattt acacaaaata
    14641 ccgccatgcc ttcttccctc tggcaatact ggcgcggcct ttccggctgg aacttctatt
    14701 ttctggttaa gttcggcctg ttgtgggcgg gatatcttaa cttccatccg ctcctcaatt
    14761 tggtgtttgc cgcgtttctg ctgatgccca ttccgcgcta cagcctgcat cgcttgcgcc
    14821 actggattgc cctgccgatc ggctttgctt tgttctggca tgacacctgg ttgcctggcc
    14881 cggaaagcat aatgagccag ggttcgcagg tggcggggtt cagtaccgat tatttaatcg
    14941 accttgtcac acgctttatt aactggcaga tgattggggc catttttgtt ttattagtgg
    15001 cctggttatt cctgtcacaa tggattcgca ttaccgtttt tgtggttgcc atactgctat
    15061 ggctgaacgt acttaccctg gcgggaccaa gtttctcctt gtggccagcc ggacaaccga
    15121 cgaccactgt aacaacgacg ggtggtaacg cagcggcaac cgttgcggcg acgggtggcg
    15181 caccggtagt gggtgatatg cccgcacaaa ctgcaccgcc aacaacggcg aaccttaacg
    15241 cctggctgaa taatttctat aacgcggagg cgaaacgtaa atcgaccttc ccgtcttcgc
    15301 tgcccgctga tgctcagcca tttgaactac tggtgattaa catctgttcg ctttcctggt
    15361 cggatataga agccgccggg ttgatgtcgc atccactgtg gtcgcatttc gatattgagt
    15421 tcaagaactt taactccgcc acctcctaca gtggcccggc ggcgatccgt ttactgcgcg
    15481 ccagctgcgg gcagacttcg cacactaatc tgtatcaacc ggcaaataac gactgctatc
    15541 tgtttgataa cctttcgaaa ctgggcttta cccagcacct gatgatgggg cataacggcc
    15601 agttcggcgg ttttttgaaa gaagttcgcg aaaatggcgg catgcagact gaattgatgg
    15661 atcaaacaaa tctgccggtt attttgctgg gctttgatgg ttcgccggtt tatgacgata
    15721 ccgccgtgct taaccgctgg ctggacgtta ccgaaaaaga taaaaatagc cgtagtgcca
    15781 cgttctacaa cacgcttcca ctgcatgacg gcaaccatta tccgggggtc agcaaaacag
    15841 cggattacaa agcgcgggcg cagaaattct ttgatgaact ggacgccttc tttactgaac
    15901 tggagaaatc gggtcgtaaa gtgatggtgg tcgtggtgcc ggaacacggc ggcgcgctga
    15961 agggcgacag aatgcaggta tctggcctac gtgatatccc tagcccgtct atcacagacg
    16021 tccccgttgg ggtgaaattc ttcggcatga aggcaccaca tcagggggca ccgattgtca
    16081 ttgaccaacc gagcagcttc ctggctatct ccgatctggt ggttcgcgtt cttgatggca
    16141 agattttcac cgaagacaat gttgactgga aaaaactcac cagtgggttg ccacaaacag
    16201 caccggtctc cgagaactca aatgcagtag ttattcaata ccaggataaa ccgtacgttc
    16261 gcctgaacgg cggcgactgg gtgccttacc cgcagtaagc acgaaaaagg ccgcagagtt
    16321 tcacccctgc ggcctggtcc gggcgcaaat tgccattacg gcagcctgac gcccgcatga
    16381 cacgttactt ccgcttgttc agccagttca cgatcatact ggcaagaatg ccagcaatga
    16441 ccggagccgc taaatcatgc cagaaggcca tgcccagttc tgcgagcgtc atatagccgc
    16501 ctgtgttgta atgacaacgt ttcgcggcta ttcttgagtt gttcaggttc aagaatggcc
    16561 cccgtgatgt tgtcaggtgc atacctgcaa cgtgcggggg ttttctctct ccagcaacca
    16621 atgccaccag ggataaagcc cccccaacat tgcgcctcac cggaaccctt ccggcttgcc
    16681 gctgattcta cgactgtttt tctgttccgg aatccgtcac gtcttcactc tgtgctgcgc
    16741 attccgcaga atattatccg cttgcaaccg ttgcattttt cttacgaaaa aggccgcaga
    16801 gtttcacccc tgcggcctga tccgggcgca aattgccatt acggcagcct gacgcccgca
    16861 tggcacgtta cttccgcttg ttcagccagt tcacgatcat actggcaaga ataccagcaa
    16921 tgaccggagc cgctaaatca tgccagaagg ccatgcccag ttctgcgagc gtcatatagc
    16981 cgcctgtatt gtaatgacaa cgtttcgcgg ctattcttgt attgtttgga tgcaagaata
    17041 gcccccgtgg tgttgtcagg tgcatacctg caacgtgcgg gggttttctc tctccagcaa
    17101 ccaatgccac cagggattaa gcccccgcaa cattgcgcct caccggaatc cttccggctt
    17161 gacgctgatt ctacgactgt ttttctgttc cggaatccgt ctcatcttca ctctgtgctg
    17221 cgcattccgc agaatattat ccgcttgcaa ccgttgcatt tttcttacga aaaaggccgc
    17281 agagtttcac tcctgcggcc tggtccgggc gcaaattgcc attacggcag cctgacgccc
    17341 acatgacacg ctacttccgc ttgttcagcc agttcacgat catactggca agaatgccag
    17401 caatgaccgg agccgctaaa tcatgccaga aggccatgcc cagctctgcg agcgtcatat
    17461 agccgcctgt gttgtaatga caacgtttcg cggctattct tgagtggtct aggttcaaga
    17521 ttagcccccg ttatgttgtc aggtacatac ctgcaacgtg cgggggtttt ctctctccag
    17581 caaccaatgc caccagggat aaagcccccg caacattgcg cctcaccgga accctttcgg
    17641 cttgccgctg attttacgat tgctttttac tgtcagcacc cggcaatctt cctcattgcg
    17701 cgatgccttc cagcttaatt tctttatttg caatagttgc agttcatatc cgggctgact
    17761 ccagactggt agtgaaatgc gcaattttgt agaattgcgc ctcctcgcga cctccccagt
    17821 gaatctgttg cctcattttt tacatactgc gtattcgact tctccacctg ttgcgcaaga
    17881 gaaactgggt ttattcattt ttgcgaggcc gacttctttc tggacaggac tttatgcagc
    17941 acaacacact atcgaaacac aatcagaaat tgccgtttac acgctacgac ttcggctggg
    18001 ttttattatg cataggcatg gcgattggtg ccggaaccgt gctgatgcca gtacaaattg
    18061 gcttgaaggg aatttgggta tttattaccg cagcgatcat tgcttatcct gccacctggg
    18121 tagtgcagga catttattta aaaacccttt ctgaaagcga ttcctgtaat gactacaccg
    18181 atattatcag tcattacctg gggaagaact ggggaatttt cctcggggtt atctactttt
    18241 tgatgattat ccacgggatt tttatctact ctctctccgt ggttttcgac agcgcctcgt
    18301 acctgaaaac cttcggttta accgatgccg atctttcaca atctctactt tataaagtcg
    18361 ctattttcgc cgtactggtg gcgattgcgt ctggtggtga acgattactg tttaagattt
    18421 ccgggccaat ggtggtggtc aaagtaggga ttattgtcgt gttcggtttt gcgatgatcc
    18481 cgcactggaa tttcgccaat ataaccgcct tcccgcaagc ctccgtcttt ttccgcgatg
    18541 tcttgcttac cattccattt tgcttctttt ctgcagtatt tattcaggta cttaacccaa
    18601 tgaatattgc ctatcgtaaa cgggaagcgg ataaagtact ggcaacccgg ctcgcgctgc
    18661 gtacccaccg aattagttat atcacgctca tcgcggtgat cctgtttttt gccttttcgt
    18721 ttaccttctc aattagccac gaagaagccg tttctgcctt tgaacaaaat atctcggcac
    18781 tggcgctggc cgcgcaggtg atccctggac atatcattca tatcacctct acggtgctga
    18841 atatctttgc cgtactgacc gcattctttg gcatttatct cggtttccac gaggctatta
    18901 aaggcattat tctcaatctg ttaagccgaa ttattgatac caagaaaatt aactcacgca
    18961 tgctgactct ggcgatctgc gcttttatcg tcattacatt gacgatttgg gtttcgtttc
    19021 gtgtatcggt gctggtgttc tttcagttgg gaagcccgtt atatggcatt gtgtcgtgcc
    19081 tcattccgtt tttcctgatc tataaagtcg cacaactgga aaaacttcgc ggatttaaag
    19141 cctggctaat tctgctttac ggcattttgc tatgcttgtc gccactgttg aagctgattg
    19201 agtaaaccgg agcgcatggc cccggttttg tgagttaacg ctgcggattt tcatcctgat
    19261 caacagcaaa acaagctacc agttgaccgc cgtagtcttt tagctgcggc tgcaactggg
    19321 tgcaggggcc gaagcgccga cgacagcggg cgttgaaggc gcaacccggc ggtggattca
    19381 gcgggcttgg cagttcaccg gtgagtttga tgcgttcgcg gcgatcgtcc gggttcaggc
    19441 gtggcgtcgc ggagagtagc gcctgagtgt acggatggcg cgggttattg aagatttggt
    19501 ctttcgttcc cttctccacg cagcggccca ggtacatcac catcacttca tcagcaatgt
    19561 gctccacaac cgacaggtcg tgggagataa agacataaga aagccccagt tcctgctgta
    19621 aatccatcat cagattcagc acttgcgcac gcactgagac gtccagcgca gaaaccggct
    19681 catcggcaat caccacatcc gggtcgagca tcagaccacg ggcaatagcg atacgctgac
    19741 gttgtccgcc ggagaacata tgcggatagc ggtcatagtg ctcggttttc aggccgactt
    19801 tcgccatcat cgacagggct ttttcccgac gctgttcttt gctgaagctg gtgttaataa
    19861 gcagcggctc ttcgagaatt tgcccaactt ttttacgcgg attcagcgaa ccgtaagggt
    19921 tctggaagac gatctggatt ttctgccgac gcagcttctg cgcctgcgga tcgtgcttaa
    19981 gcagatcctg cccctggtaa tacagctcgc caccggtggg catttcaatc atcgtcagca
    20041 accgaccgag ggtcgattta ccgcagccag attcgcccac tactgccagc gttttgccac
    20101 gttcaaggtt aaacgaaacg ccatccagcg ctttaaccag acgttccggc gcgaacatgc
    20161 ctttcttcac cggataatgt tttttcaggt cgatagcctg caacagcggt tgttgcgagg
    20221 tggcctcttg cgtactcata gtgtcggcct cccggcatca tcaagtgggt aatggcattt
    20281 ggactgacgc ccatcagcga gcatattcag cgccggttct tcagcgcgac atctgtcagt
    20341 ggcatagggg cagcgcgggt taagcaggca gccgttcggg cggtcgtact taccgggaac
    20401 gacacctggc aacgacgcca gacgttcttt gtcctgagca aattctggca gcgcacgcag
    20461 caatgcctga gtatacgggt gacgcggcgc atggaagatg gcgtgcgcat caccggtttc
    20521 caccacctgg cctgcataca tcacgatgat tttatgtgcc gcttccgcca ccagcgccag
    20581 gtcatgggta attaacacca gcgccatgtt ctctttctgc tgtagctcca gcagtagttc
    20641 gatgatttgc gcctgaatgg tcacgtccag cgcggtggtc ggttcatcgg caatcagcag
    20701 ttttggccga caggcaatcg ccatggcgat catcacgcgc tggctcatgc cgccggaaag
    20761 ctgatgcggg taaacatcca gacgcgatgc cggatcggga ataccgacct gattcagcag
    20821 gtcaatcgct cgctgacggc gggtactttt gttgccgccc tgatgcacct taatcgcttc
    20881 cataatctgg aaacccacgg tgtagcacgg gttaaggctg gtcatcgggt cctggaagat
    20941 catcgccact tcggcaccca ccaggttgcg gcgctctttt tctgagatac gctgcaaatc
    21001 ctggccgtta aactccagtt tttctgccat tacgcggccc ggataatcaa tcagccccat
    21061 aatcgccagt gaactgaccg acttaccgga gccggactca cccacaatcc cgaccacttc
    21121 gccctgtttt acgctgtagc tgatgcggtc tacggcgcgg aacggcgcgc tttcgtcgcc
    21181 gaaatgcacc gataatttat ctacatttaa taacgccatc tcgaacctct tactgcttca
    21241 gtttgggatc gagcgcgtca cgcagaccgt cacccatcag gttaaatgcc agcaccgtca
    21301 gcaggatcgc cagacccggg aaggtcacga cccaccaggc gctttgcgcg aactgcaaca
    21361 cgtcggagag catggtgccc cactcaggcg ttggcggctg tgcccccatg ccgaggaaac
    21421 caagagcagc catatcgaga atggcgttag agaaaccgag cgacgcctga acaatcagcg
    21481 gcgcaaggca gttcgggaag atgttaataa acatctgacg catcgccccg gcacccgcca
    21541 cgcgagacgc ggtgacgtaa tcgcggttaa cttccaccag cacggcggcg cgggttaagc
    21601 gcacatagtg cggcaaggca acgaaggtca gtgccagcgc ggcgttacca atcgacgggc
    21661 cgaaaattgc caccagcacc agcgccagca gcagacttgg cagcgccagc atgatatcga
    21721 ccacgcgcat aatgatgtta tcgaccaggc cgccaaagta accggcgatc aggccgagaa
    21781 taacgcccat aatcagcgat aacacaacta ccagacagcc aaccagcagc gacaggcgcg
    21841 caccgtacat caggcgcgac agcacatcac ggcctacgtc atcggtgccc agcaagtgcg
    21901 ccatgctgcc gccttcctgc caggctggcg gggcgagcag tgcatcgcgg aactgttccg
    21961 ccgggttata gggtgcaatc cagttggcaa agatcgcgat gaacagcacg atgacgacgt
    22021 aaaccagccc gacgaccgcg cctttgttgc gtttaaaata gtgccagaac tcctgtaacg
    22081 gggtcatcgg caccggtgcg ctaatcactt tattttcagt aacctgtgac atgatgtccc
    22141 cttacttctt atgacgaata cgcgggttca ccacgccgta cagcagatcg accagcaagt
    22201 tgacgaggat aatcatcgtc gccaccagca atacgccgcc ctgcactacc ggatagtcgc
    22261 ggcgttgcag tgcgtcaatc aaccagcgtc ccagaccggg ccacgagaag atggtttcgg
    22321 tcagaatcgc ccccgccagc aatgttccca cctgcaggcc gataacggtc accaccggca
    22381 gcatcgcgtt acgcagcgca tggacgataa tcacccgcat gcgggtcagc cctttggcgc
    22441 gcgcggtgcg gatgtaatcc tcgcccagca cttccagcat cgaggagcgt gtcatacgca
    22501 caatgaccgc cagcggaata gtacccagca caatggcagg caagatcata tgggcgacgg
    22561 catcgataaa gttgccgtct tcaccccaga tggcggtgtc gattagcata aaaccggtta
    22621 acggattgga gtcatcgagg aacaccatat cgctcacgcg accggagacg ggcgtcaggt
    22681 tccagtgcac cgaaaccagc atgatcagca tcatgcccca ccagaagatc ggcatcgagt
    22741 agccggtcag cgccaggcca actgcagtgt gatcgaaaat ggaaccgcgt ttaaccgcag
    22801 ccagcacacc aaccggaata ccgactgccg tagcaaaaat catcgcgcag acgccaagtt
    22861 ccagcgtggc ctgaaagcgc ggcacgaact cttcccatac tggaattcgg cttttcattg
    22921 aaatgcctag atcgccgtgc attacgcccc aaatgtaatg gagatactgc tgccacatcg
    22981 gtttatctaa gccgagttca gccagcagct gcgcgtgacg ctcaggggag atcccacgtt
    23041 cgcccgccat gatcatcacc ggatcgcccg ggatcatgtg gacaaaggca aatgtgagaa
    23101 gggtaatacc gataaacgtg gggatgacga gtcccaaacg tcggagaata aactgcaaca
    23161 taacccggat tctctataat gacccgcagc ctggaactgc gtgtctgtat tgctcacaaa
    23221 tcaattcccg cccttgtcgg acagagaatg aagtattgct gccggatggc gctgcacgca
    23281 gaggcacagc gccaccgggt atggctttta attattcgat agagacgttt tcgaagtgat
    23341 gtttgcctaa tggatcaacc acatagcctt taacttcttt acgtaccggt tcaaacacgg
    23401 tggagtgagc gatgatcagt gccggagcct gatcgtgcat caccacctgt gcttgtttgt
    23461 acagttcaac gcgtttgtta tggtcgtcgg tagcacgcgc cggttgaatc agatcttcaa
    23521 acggtttgta gcaccatttt gagtagttgg agccttgttc agaggcggcg cagctgaaca
    23581 gggtggcgaa gaagttatcc ggatccccgt tatcgccagt ccagcccatc attaccgtct
    23641 ggtgctcgcc atctttcgca cgcttgaggt actcacccca ttcgtaggtg acaattttgg
    23701 cctgcacgcc gactttcgcc cagtctgcct gaatcatctc cgccatgcgg cgagcgttcg
    23761 ggttatacgg acgttgtacc ggcatcgccc acaggtcgat ggagaaacct ttttccagac
    23821 ccgcttcttt cagcaaggct ttcgcttttt caggatcgta ggtgtagtcc tgaacgtcgt
    23881 cgttatagcc ccacatggtt ggcgggatca ggtttttcgc tgatacaccc gcgccctgat
    23941 aaaccgcttt gatgatcgcg tctttgttca ccgcgtaggt cagagcctgg cgaactttta
    24001 cgtcatcgag tggttttttc tgcacgttat atgaaagata gccgacgttc agccccggca
    24061 tttccatcag attgatggat ttatcctgct tcatgcgagc gatatctgcc gggttcgggt
    24121 acggcatcac ctggcattca ttcttctgca atttcgcgta acgcacggaa gcgtcagggg
    24181 taatagagaa gaccagcgta tcgatctgcg gtttggtgcc ccagtagcca tcaaacgctt
    24241 tgtagcggat acgggaatct ttttgatact gctgtaactg gaacggaccg gtgccgattg
    24301 ggttgaggtc cagtttttcc ggtgtaccgg ctttcatcat cgcatcagca tattcttttg
    24361 acagaataga ggcgaagtcc attgccaggt cagcgaggaa cggcgcttcc gggcgagtca
    24421 gcacaaactg aactgtgttg tcgtccacct ttttcacttc gctgatcagc tccggcaagc
    24481 ccatgccttc gaagtattcg tagctgccgc cagaaacttt atggtacggg ttttgcgcgt
    24541 ttttctgacg atcgaacgag aacaccacgt catcggcgtt cagttcacgc gtcggtttga
    24601 attctttatt gtcgtgccac ttcacacctt tacgcagatg gaaggtatag gttttaccgt
    24661 cttcgctgac ttcccacttt tcagcgaggc ccggaatcac ttcggtggtg ccgattttaa
    24721 attcaaccag gcggttataa agcggtacgg aagaggcgtc ataggtggta ccggaggtaa
    24781 acagctgcgg gttaaaccct tccggagatc cttctgagca ataaaccaga gttttagcct
    24841 gaacacttgc tgcgacggtc atagccacca ggctgagacc aagcttcagc atccctgact
    24901 ttttcaagga aatacgcatt attctgctcc aattgtgatg tttgttgttt taaccctttg
    24961 cagtgggttg tcgctgcctg accttttttg ttttttgccc ggtcgggtca acgttatgag
    25021 gtggggatgc cgtactaatt aacatcagtg taacagtgcg gatcctccat aaaatgcccc
    25081 tcgtgaccta caatctgtca acagaatgtg aaaacgtcaa tacagccaac cgggatttac
    25141 accaacggtg agaatccaca aacaaagatt aaaaaaactt caaacagcta tttgcagcag
    25201 agaaatttgt gctactccaa catgaccaga acaatcagct taatatttag caacatttgg
    25261 tgattaagtt ttatgcattt agtgaaaaaa atctgaggaa aaggtggata tctgagcgat
    25321 taataccatg aacgctaaaa cgcacagcgg aaaatgccag cgccagccat aagttacgaa
    25381 aaaaaagatt taagcaaata taaaaaaaga caatggtttc tgtcacaaat ccgttaatgg
    25441 atagtgagat atggggcgca aatttggcag ggaaatgtgt ggcgtgtagg tcgaataagg
    25501 cgttcacgcc gcatccgaca ataagtgcgg tgccgttgcc tgatgcgacg ctgacgcgtc
    25561 ttatcaggcc tacgtcgagt cgaacctccc cgcccccgga ggttctcatc ctcatgagct
    25621 acaaatgcaa aaaagcctgc tcgttgagca ggcttttcga atttggtcgg tgatagagga
    25681 ttactcgcca cttcgtggct cgccctgcgg gccgttgctg gcgcaacgtt ctctcgcttc
    25741 gctcgagtcg aacctccccg cccccggagg ttctcatcct catgggcttc aaatgcaaaa
    25801 aagcctgctc gctgagcagg cttttcgaat ttggtcggtg atagaggatt cgaacctccg
    25861 accccttcgt cccgaacgaa gtgcgctacc aggctgcgcc aatcaccgaa tgcggggcgc
    25921 atcttactgc gcagatacgc cctcgtcaat cccttaatag caaaatgcct tttgatcggc
    25981 gagaaagtca gcaggccgct tagttagccg ctgccacttt tgtctgcggg atgtgacacc
    26041 agttgttgtt ttcgttaatt ccaccatccg gtgaagtata gccaagacaa cccatgatgg
    26101 tgtcgtacag ctctacatga cggcgtggca ctttcatgtc ggcttctttt ttcagctgcg
    26161 caaatgcctg agcattggcc ggattttcca gatatttatc tgacatccag accatcatcg
    26221 gtacgcggaa ctgctccggc ggtgccagtt cacgcggcgt gccgtgcagg tgctcgcgtt
    26281 cattaattga ctcaccgtgg tcagctgcgt agaacacaat cgctttctta tcgcgaacct
    26341 gatcaatcac gctggagata aagtgatcca cataggtcac cgagttgtca taggagttga
    26401 tcatctgcgc tttggtacag ccgctatcaa caccaataca ttccggcttc cactgcgcga
    26461 agctacgcgg ataacgctgg gtgtagttaa aatgcgaacc tttggtatgc agaatgatca
    26521 gatgcttacc atccgggttg cgccctagcg attgctgcat ttcgtctacc agcaacatat
    26581 catctaccgg cttgccacga ttacgtggct ccgcaccaat ctgctcacga taagcaatgt
    26641 tgtccgccat cgtgttgctg tagaaccaca tttcgctctg catagcgtag aggtcagaac
    26701 tgaatcctaa ctgcttcaga accgcgaaaa tgttctgttc ttttaatgtg cgctgcggat
    26761 tatcttccgc gcccccctga cgtacaaaca tgcaacgcag tgagagtttg gttgcggtat
    26821 cacaggagta accacggaac gccgccagat ttttctcctg ggccagtttc ggcgtggtat
    26881 tacgctcata gccgaaaatg cccatatggt cccaacgcgt ggtttcaccg atgataaaca
    26941 cgacataagt gtcatcaacg ttttgcggtg cctgataagt gaatttcttc gccggattaa
    27001 gcaatgaatt attatcggaa gattcatcga cccgcgccca ggcatacagc cccaacgcag
    27061 aaagccagtt tgatggcaga taagagttcg ctacgacacc gccataactc ggcaaatcaa
    27121 cgccggtcgc cctctccact ttcttctgct ggatatccag caaacgaatc ggtgcccaaa
    27181 ccataatacc cgccagtacg acgaccgcca ggctgcgaat acgctgccct ggggttcgca
    27241 gttgtcggag caaggtgtag cgacagcggt tattccagat aaggataaga ggcaatgcac
    27301 taacggcgat taaccagaga ataaagttca gaccaacaac ttcttttgac aggtcgatat
    27361 cggtggtcat gacggaagcg atgatgccat aaccaatgac cacattaagg aaggtcatgt
    27421 aatagctggc acctgcggaa aagagcacca ccagcgacgc cagaatacgc cagctgcggc
    27481 ggccaaacag cgatagaaga cgtagtaaaa agaaggtcac cagtacggtg gcggccagtt
    27541 caacaacagc agaaacgcct ttccagacgg taaaatcgtg cgcatagctg ccgaagcggc
    27601 gataaaaaac cgcgccattc ataaaaaggc caatatagat tgcaagcaaa aagctcagct
    27661 tctgctgtgt aatcgatttg atgtatctca tgcaaacaaa ccctggacaa acaggtgata
    27721 acaccctgct tagcggcttt tcaggcccgg caggggaaaa gtgcggaggt agtcagataa
    27781 gtaaaatcta cacgcgcgaa taagtagacc acagcaaagc gaaaaagtgt ggggaaagag
    27841 tgtgcatgaa gcacaaattt acaaaaatgc aagtttgaaa agagacttta gcgttacaaa
    27901 aagcagtaaa aagggccagt taactggccc aaattttacg agtcattgtt tatacatttc
    27961 gttgatgcgt ttgatgcaca cggaagctga agcccagtag ctcgcggctg agcattaaag
    28021 ggagccatgc gcctcacgca acattttctg ctctggctga cgaattgtcg tagaaagcgc
    28081 caatgacaga atcagcaggg cgaaaatcac gtagaaggtc acatagaagc cgccaaacag
    28141 tgaggcgata atcgaaccaa aaatgctacc gataccgaaa ccgaggtaaa tcacaccgta
    28201 gtttttcgcc aggttattga ggccgaagaa ctcactgacc agtgacggaa agacggtaat
    28261 agtgccgcca aagttaaatg ccacgcaggc aatcgctgca aagaacgtca ctgcattcag
    28321 tggtgcaaac agcagggccg ccatacccac tagcgaaatc acctgaccaa tggtgataac
    28381 acggatacgg gcgattttgt cagacagtat ccccagaacc agacgacctg aaaggttggc
    28441 gatggaaata acagtgactg cattagctgc ggaaaccaca tcaaggtgtg ccagactttg
    28501 ggcgatatct ttcgctaccc caatcacgta caggccgctc atgcaggcag tcaggaacat
    28561 taccgctaac atccagtact gcggtttacg catcgactct gccagcgtgt aatctttctc
    28621 caccacacca ttgctggttt tcacttcctg ttttggtgcg tctttcatta acgttgcgcc
    28681 gaaaacaatc atcaccagcg caatcgctcc ccaaatcaca aaggtttttt ccagacccac
    28741 cgtttccagc agctgcgtgt cgataaattt gaaacccagg ctacccagac cataagaacc
    28801 gatagcgaac gcggatatca gacctttacg ctccgggaac cacttcacgc agttagagag
    28861 cgtcagcagg taacccgcgc catccgccag accgaccagc acaccggcgc ttaaccacag
    28921 catcatcagg ttgttggaat gcgcggtcag gaagaagcct aatcccagca aaatgccgga
    28981 agccatggtg acgcgtttaa cgccaaaacg ttcctgcaat ttgcccgcaa cagaagacga
    29041 aattgccagc cccagactta acaagccgaa agagaaagcg acctggctta ccggcgcatc
    29101 cagcttggca gaaagcgcgc cattaaacag gctccaggta taaaccgacc ccagtgcaaa
    29161 ctgggtaatg atagtaccga tgagtgtcag ccagcgggta cgctgataat ttgaaggtgt
    29221 catggcagta ttcctgcagt aataaaaagg aaaaatctct gccgacaacc ataacgaagt
    29281 gcattttatg cctggggaaa aacggcatga aatgccatca gaacggaatg aaatgcctta
    29341 gttcaggaat gaatgacgcg acaattattc accaaattta tcattttgta agttagcacc
    29401 cactatcacc acaagcattg ttattcaatg gtttcaattg tgactgttgc gttaaaatct
    29461 ggcactaacc cccatcgtat acagatagtc gctattgtta gtggtatttt ctgcctgaga
    29521 tagcgcggca taggcagcaa tattttgatt gagcaacata tcagcgccta cggtgacatc
    29581 cagccagttg ccgttctggt ttgtcgccgt catccggctt aagcctgatt gcgccttcca
    29641 gatattctcg ccaaattgct ggttatagct gatttgcgcc cagggtcgga gatcgccaag
    29701 ccgggagtca acacgccagc ctaagctact cacgctggca tgccataacg gatcggtcag
    29761 cgtggtagta ctgtcgccaa actcgttata catcgaggtg gttgtgccat cgtaatgtaa
    29821 tgccgcgacc gggccggttg taacctgttc atataaagga aaattccagc ccatactcat
    29881 actgctggtc acatccacag gagtttcttc cgccagattg acttccagtc ccagcgccct
    29941 ttggctactt tgaatacgtt gcgacaaaat atcgttgtaa tcaggttgat gatcactatc
    30001 ccaggtgtaa cgctctgtgg aatgtccggg ttgcgtatca acgatatatt cttgttgcca
    30061 ggcgtaagct gtgttgagaa aaaaggcact gattaccacg ctcgccagca ggcttagctg
    30121 ccagcgacca ccgctttttt ttatgatcat taacacgact ccagggaaga gccgaattcg
    30181 gcaatattat tgtcattgta tgaaggatat cgggcatagt agccctgtat taaatattga
    30241 ctttttcacc gatgcgtcaa gaaaagcggc tgaaattttt acgatcgggt acatagcgag
    30301 ggaaagtatg gaacgttgcg gctgggtgag tcaggacccg ctttatattg cctaccatga
    30361 taatgagtgg ggcgtacctg aaactgacag taaaaaactg ttcgaaatga tctgccttga
    30421 agggcagcag gctgggttat cgtggatcac cgtcctcaaa aaacgcgaaa actatcgcgc
    30481 ctgctttcat cagttcgatc cggtgaaggt tgccgcaatg caggaagagg atgtcgaaag
    30541 actggtacag gacgccggga ttatccgcca tcgcgggaaa attcaggcaa ttattggtaa
    30601 tgcgcgggcg tacctgcaaa tggaacagaa cggcgaaccg tttgccgact ttgtctggtc
    30661 gtttgtaaat catcagccac aggtgacaca agccacaacg ttgagcgaaa tccccacctc
    30721 tacgcccacc tccgacgccc tatctaaagc actgaaaaaa cgtggtttta agtttgtcgg
    30781 taccacaatc tgttactcct ttatgcaggc atgtgggctg gtgaatgatc atgtggttgg
    30841 ctgctgttgc tatccgggaa ataaaccatg attcgggaag cgcaacgttc agaactcccc
    30901 gcgatcctcg aactgtggct ggaaagtaca acctgggggc atccctttat aaaagcgaat
    30961 tactggcgtg attgcattcc gctggtgcgg gatgcgtatc ttgccaatgc gcaaaactgg
    31021 gtctgggaag aagacagtaa gcttctcggt tttgtcagca ttatggaagg ccgatttctg
    31081 gcagcgatgt ttgtcgcacc gagggccgtc aggcgcggta ttggtaaggc gctgatgcag
    31141 tatgtgcagc agcgccatcc ccatctgatg ctggaggttt atcaaaaaaa tcaaccggcg
    31201 atcgattttt accgtgcgca gggttttcac attgtcgatt gcgcatggca ggatgaaacc
    31261 caactaccca cctggattat gagctggccg gtggttcaaa cgctgtaagt gtcagttccg
    31321 gaccgttgta tttttccagc catgccagcg ccgtattacc cgcacagcca ttccccagcc
    31381 gcgagctggg gagatctttg gtcagcacgt tcactgcgcc gtttttacaa ataccgtcag
    31441 cggttaaatc cagatccggc catgcccctt cgtgaatgca aatcacgcca ggtttaattc
    31501 cctcgctaat gaccgctccg gcaagaattt gcccgcgtgc gttccacaac cgaacagtat
    31561 cgccatcttg tatgccgcgc gcctgagcat cgtcaggatg aatggtgaca ggctcacgat
    31621 ttgccaccgc gtacaattcg cgcagagaac tgtaattcag ctggctgtgc agacgatgtg
    31681 ccggatgagc agaaagtacc tgtaactgtt ccggttcggc attgccctgc cattcgtccg
    31741 gctccagcca cattggatgc cccgggcaat ccggataagc gtaatcggca atacgctgtg
    31801 agaagatttc aatcttaccg ctggcggttt ttaatggatg cgcctgcgga tcgcggcaaa
    31861 aatcggcgaa gcgaataaac cgctcgctgt ccgggttttc cggcatctca attaactgat
    31921 tggcttgcca gaactcagca aatggcggca attcaacctg ctggcttgcc ccgcgctgtc
    31981 gggcaacgtt ataaaacgtt tccagccatt gcagctcact ttttccttcc gtaaaacgtg
    32041 cataaccgcc cttctcccag cgttcactta actcggcaaa aacatcaaaa tcattacgcg
    32101 cttcatagcg tggcggcacc acttgcttca tcggcaccag atgctgatta ctgtaatcac
    32161 cggtcatggt gagatcatta cgctcaaaag aggtagtcgc aggcagaacg atatccgcgt
    32221 gtttcgccgc cgccgtccag aagcattcag agatcaccac cagctccggt ttttgccagg
    32281 cacggatcag gcgattggta tcctgatgat gagtaaagtt ggcaccgccc gcccaccaga
    32341 taaaacgaat atccgggaaa tgtcggtcca taccgttgtg ttgatatgcg ccaccggggt
    32401 tttccagtgc ttcaacaatg cgggccacag ggattttatc taccgcatcg cagccacccg
    32461 gcaagctgcc ctgcatggaa gagagtaccg cagcgcgccg cgtggggtta ccaccattgg
    32521 caaaatggta agaaagacca aaaccgccgc cgggtgtgcc gatttgcccc aacattgcgg
    32581 ccagcgtgac gatcatccag tgtttttgct caccaaactg ttggcgctgc attccccagc
    32641 ctgccatcag catggtggta ttttggtgga aaatagccgc cagctcgcgg attttcgctg
    32701 cgccaacacc acaaatctct gccgcccatt cggcgttttt cgctattccg tcactctcgc
    32761 ccagcaaata agaggcgaag acggcataac ctgtggtgca acgcgccaga aacgcttcgt
    32821 cgtgccaacc attttccacc agcgtatggg cgatccccag catcagcgca acatcggtgc
    32881 ccatgtgcgg tgccacccac tccattttat cgccaaagaa atcgacggtt tccgatcgca
    32941 tcggatcaat gcagatcagc tttttcccgc tgtcacgcag tgcagaaaag taagaaagcc
    33001 cctgctcatc agatgcattc cacgcaattt tcagcgtatt gagtggatta gcactccaca
    33061 gcaccacgac atcgctatgt tccagcacca gcggccaact ggtctgctgt tgataaactt
    33121 cgctaccacc cacgacatac ggcatgatcg cctgtgccgc gccggtcgaa taatccccca
    33181 gatgcccggt ataaccgcct gccagcgcca tatagcgttg taataatgtc gaggccttat
    33241 gcagcacgcc gtttgaacgc cagccgtagg aaccagcaaa aatcgatgcc ggaccataag
    33301 cctcacgaat gcgtttatgt tgttggtgaa taagatccag cgcctcatcc caactcacgc
    33361 gaacaaattc atcctgccca cgaatgcctt gcgggttttc cggtgacgcg agaaagcctt
    33421 tgcgcaccat tggaaatcgt acccgcgtat tgctgtgaac ctggtcgcga accgcgctct
    33481 gcaaggagtt ttccattcct gtggctaacg cgccacgcga gctaaacacg gtttcgccgt
    33541 cggtttcaac cagcatgggc ccccagtggg cggcagtcag aacggaatat cgtgaggatg
    33601 agttggccaa accgaagact cctgaaaggt tagatgtatt gagcagttaa atattactta
    33661 caaatttccg agtattccca ggaataatct tcatgttcac gcggcataat ctcccgccac
    33721 ggaacccgtg gcaaagaata aaaaggttat taaggattaa caatgaagaa acgtgtttat
    33781 cttattgccg ccgtagtgag tggcgctctg gcggtatctg gctgcacaac taacccttac
    33841 accggcgaac gcgaagcagg caaatctgct atcggcgcag gtctgggctc tctcgtgggc
    33901 gcgggtattg gtgcgctctc ttcttcgaag aaagatcgcg gtaaaggcgc gctgattggc
    33961 gcagcagcag gcgcagctct gggcggcggc gtgggttatt acatggatgt gcaggaagcg
    34021 aagctgcgtg acaaaatgcg cggcactggt gttagcgtaa cccgcagcgg ggataacatt
    34081 atcctcaata tgccgaacaa tgtgaccttc gacagcagca gcgcgaccct gaaaccggca
    34141 ggcgctaaca ccctgaccgg cgtggcaatg gtgctgaaag agtatccgaa aacggcggtt
    34201 aacgtgattg gttataccga cagcacgggt ggtcacgacc tgaacatgcg tctctcccag
    34261 caacgtgcgg attccgttgc cagcgcgttg atcacccagg gcgtggacgc cagccgcatc
    34321 cgtactcagg gccttggccc ggctaaccca atcgccagca acagcaccgc agaaggtaag
    34381 gcgcaaaacc gccgtgtaga aattacctta agcccgctgt aatccctttc atgccaggtg
    34441 atgcagaaat cacctggcat tcttcagttt tccttcatca tatttcaggc taaggtgatc
    34501 gccttatcag tgaatggaga gaagcatgaa gccgtccgtt atcctctaca aagccttacc
    34561 tgatgattta ctgcaacgcc tgcaagagca tttcaccgtt caccaggtgg caaacctcag
    34621 cccacaaacc gtcgaacaaa atgcagcaat ttttgccgaa gctgaaggtt tactgggttc
    34681 aaacgagaat gttgatgccg cattgctgga aaaaatgccg aaactgcgtg ccacatcaac
    34741 gatctccgtc ggctatgaca attttgatgt cgatgcgctt accgcccgaa aaattctgct
    34801 gatgcacacg ccaaccgtat taacagaaac cgtcgccgat acgctgatgg cgctggtgtt
    34861 gtctaccgct cgtcgggttg tggaggtagc agaacgggta aaagcaggcg aatggaccgc
    34921 gagcataggc ccggactggt acggcactga cgttcaccat aaaacactgg gcattgtcgg
    34981 gatgggacgg atcggtatgg cgctggcaca acgtgcgcac tttggcttca acatgcccat
    35041 cctctataac gcgcgccgcc accataaaga agcagaagaa cgcttcaacg cccgctactg
    35101 cgatttggat acactgttac aagagtcaga tttcgtttgc ctgatcctgc cgttaactga
    35161 tgagacgcat catctgtttg gcgcagaaca attcgccaaa atgaaatcct ccgccatttt
    35221 cattaatgcc ggacgtggcc cggtggttga cgaaaatgca ctgatcgcag cattgcagaa
    35281 aggggaaatt cacgccgccg ggctggatgt cttcgaacaa gagccactgt ccgtagattc
    35341 gccgttgctc tcaatggcca acgtcgtcgc agtaccgcat attggatctg ccacccatga
    35401 gacgcgttat ggcatggccg cctgtgccgt ggataatttg attgatgcgt tacaaggaaa
    35461 ggttgagaag aactgtgtga atccgcacgt cgcggactaa gccgcgactg cgtggagtaa
    35521 agcccgataa tcgctcgggc ttttactctt tattgggttg cagtaactgc tgtagtccag
    35581 gcctgattaa acgcctgatg ttgtgccggt aatggcgcaa tcagtttgtt atattcactt
    35641 gcctgctgtg aagtcgggaa ctggatgcca tttgcgacaa agctcacctg agtaccttgc
    35701 tgagcaatgt agtctccaac catcaccagt tgttgcgtga aagtttgtgc agccggaatc
    35761 aacggttgca atgcatcagc tggcgtcgtt accactttgg tgaatgcctg atcaaagacc
    35821 ggtttcaaat catcggtctg ttttagcgca gagtgcgcag catcggcctg caacttcgca
    35881 ttttgcagtt gttgcgccag cacacccagc gaaccgttca tttcacgcag cggaccgctt
    35941 tgcgtaacgt aatcctgcgg tacgcgaatt gcgttaacgc tgtctacgac cgggcgcaga
    36001 ccggaatcca tcgcctgatt cacctgctga gaataaccat acagaatcgc gtaatcagag
    36061 acaaaaggac caaactgttt tttctgatcg gcagtcaggg ttggtagacg ttcaccgcta
    36121 cgcatcactg tattctgcag gaagtcgata aacgctttac gctgatcgcc ttctttatca
    36181 aaacacccac tcaggctaac taccattaat aacgccgcaa gaggcgcaaa ccagcgagag
    36241 caggactttc ctgtcgccat tttcttactc ctttcaccca aaaaaagcgc acaacgacac
    36301 acgcgtgtct gacgtgacaa ggatagtcca ggacaggctt gcaggatacc ctttctacgt
    36361 aatcttcctg gtaaaatcat ggagaaaatg aaacaaggtt aatttgttgt ttcattgtta
    36421 aaaaatgata gtgccgttgc cttttttgta gccagagatt tacgccttcg cagaaggtta
    36481 gattttcgaa atctttacaa aacaggagtg gtaagagata tatcggcatt tatcagatcg
    36541 ttaggtggct ataagtcacg ctaaatgaca ggctgaatcg aatcatagcc agagcatgcc
    36601 ctgacttcac cccgctgtgt ctgcttttcc cgactattct taatgagctt cgatgcaatt
    36661 cacgatcccg cagtgtgatt tgaggagttt tcaatggaat ataaagatcc aatgcatgag
    36721 ctgttgagca gcctggaaca gattgttttt aaagatgaaa cgcagaaaat taccctgacg
    36781 cacagaacaa cgtcctgtac cgaaattgag cagttacgaa aagggacagg gttaaaaatc
    36841 gatgatttcg cccgggtttt gggcgtatca gtcgccatgg taaaggaatg ggaatccaga
    36901 cgcgtgaagc cttcaagtgc cgaactaaaa ttgatgcgtt tgattcaagc caacccggca
    36961 ttaagtaagc agttaatgga atagactttt atccacttta ttgctgttta cggtcctgat
    37021 gacaggaccg ttttccaacc gattaatcat aaatatgaaa aataattgtt gcatcacccg
    37081 ccaatgcgtg gcttaatgca catcaacggt ttgacgtaca gaccattaaa gcagtgtagt
    37141 aaggcaagtc ccttcaagag ttatcgttga tacccctcgt agtgcacatt cctttaacgc
    37201 ttcaaaatct gtaaagcacg ccatatcgcc gaaaggcaca cttaattatt aaaggtaata
    37261 cactatgtcc ggtaaaatga ctggtatcgt aaaatggttc aacgctgaca aaggcttcgg
    37321 cttcatcact cctgacgatg gctctaaaga cgtgttcgta cacttctctg ctatccagaa
    37381 cgatggttac aaatctctgg acgaaggtca gaaagtgtcc ttcaccatcg aaagcggcgc
    37441 taaaggcccg gcagctggta acgtaaccag cctgtaatct ctgcttaaaa gcacagaatc
    37501 taagatccct gccatttggc ggggattttt ttatttgttt ttcaggaaat aaataatcga
    37561 tcgcgtaata aaatctagta ttattttt
//
LOCUS       DAZFRN010000033        37357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFRN010000033 DAZFRN010000000
VERSION     DAZFRN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.033"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            101..385
                     /gene="pduA"
                     /locus_tag="ACHKUB_001596"
     CDS             101..385
                     /gene="pduA"
                     /locus_tag="ACHKUB_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFO6654629.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAVAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            382..1194
                     /gene="pduB"
                     /locus_tag="ACHKUB_001597"
     CDS             382..1194
                     /gene="pduB"
                     /locus_tag="ACHKUB_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFO6654630.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPETTHQTRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPQGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1213..2877
                     /gene="pduC"
                     /locus_tag="ACHKUB_001598"
     CDS             1213..2877
                     /gene="pduC"
                     /locus_tag="ACHKUB_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFO6654631.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2887..3555
                     /locus_tag="ACHKUB_001599"
     CDS             2887..3555
                     /locus_tag="ACHKUB_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFO6654632.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVSSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3570..4088
                     /gene="pduE"
                     /locus_tag="ACHKUB_001600"
     CDS             3570..4088
                     /gene="pduE"
                     /locus_tag="ACHKUB_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFO6654633.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4098..5930
                     /locus_tag="ACHKUB_001601"
     CDS             4098..5930
                     /locus_tag="ACHKUB_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFO6654634.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPGGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLISWHRELAHGK"
     gene            5920..6270
                     /locus_tag="ACHKUB_001602"
     CDS             5920..6270
                     /locus_tag="ACHKUB_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFO6654635.1"
                     /translation="MESKEHTPAIVVTEIGDCISTWNEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWQAARKSPLLVGIACDREKLIVHYKNLPASAPLFTLMYQQDNHTRRSIGNNA
                     ARLVKGIPFRECHS"
     gene            6290..6565
                     /gene="pduJ"
                     /locus_tag="ACHKUB_001603"
     CDS             6290..6565
                     /gene="pduJ"
                     /locus_tag="ACHKUB_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFO6654636.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6589..7011
                     /locus_tag="ACHKUB_001604"
     CDS             6589..7011
                     /locus_tag="ACHKUB_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFO6654637.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            7014..7646
                     /locus_tag="ACHKUB_001605"
     CDS             7014..7646
                     /locus_tag="ACHKUB_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFO6654638.1"
                     /translation="MDKQTLQETVNQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7643..8134
                     /gene="pduM"
                     /locus_tag="ACHKUB_001606"
     CDS             7643..8134
                     /gene="pduM"
                     /locus_tag="ACHKUB_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFO6654639.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGLHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHRDRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8138..8413
                     /locus_tag="ACHKUB_001607"
     CDS             8138..8413
                     /locus_tag="ACHKUB_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFO6654640.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRISADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8425..9432
                     /gene="pduO"
                     /locus_tag="ACHKUB_001608"
                     /pseudo
     CDS             8425..9432
                     /gene="pduO"
                     /locus_tag="ACHKUB_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
     gene            9429..10814
                     /gene="pduP"
                     /locus_tag="ACHKUB_001609"
     CDS             9429..10814
                     /gene="pduP"
                     /locus_tag="ACHKUB_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFO6654641.1"
                     /translation="MNNSELETLIRNILREQLVPATPRTQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITTIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VASVADYLIQQMQSFGALLLNYEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDANDSWVTCEQLMPMLPIVKVNDFDSALTLALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10825..11937
                     /locus_tag="ACHKUB_001610"
     CDS             10825..11937
                     /locus_tag="ACHKUB_001610"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFO6654642.1"
                     /translation="MNTFSLQTRLYSGPGSLKVISRFSHKHIWIICDVFLARSPLINT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     IVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLQHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11934..13277
                     /locus_tag="ACHKUB_001611"
     CDS             11934..13277
                     /locus_tag="ACHKUB_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6654643.1"
                     /translation="MNVKLTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNITRAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRLQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGTLNPADPMANYRLIPVKQLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13280..13834
                     /gene="pduT"
                     /locus_tag="ACHKUB_001612"
     CDS             13280..13834
                     /gene="pduT"
                     /locus_tag="ACHKUB_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFO6654644.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLTAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13834..14184
                     /gene="pduU"
                     /locus_tag="ACHKUB_001613"
     CDS             13834..14184
                     /gene="pduU"
                     /locus_tag="ACHKUB_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFO6654645.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14189..14632
                     /locus_tag="ACHKUB_001614"
     CDS             14189..14632
                     /locus_tag="ACHKUB_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO6654646.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NLEQISLVKCWLREAGAHNVLVTSAVNNNGVAELLALLHIEEGCR"
     gene            complement(14733..15062)
                     /gene="tmaR"
                     /locus_tag="ACHKUB_001615"
     CDS             complement(14733..15062)
                     /gene="tmaR"
                     /locus_tag="ACHKUB_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO6654647.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15234..16292)
                     /gene="yeeA"
                     /locus_tag="ACHKUB_001616"
     CDS             complement(15234..16292)
                     /gene="yeeA"
                     /locus_tag="ACHKUB_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6654648.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQRMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16491..16964)
                     /gene="sbmC"
                     /locus_tag="ACHKUB_001617"
     CDS             complement(16491..16964)
                     /gene="sbmC"
                     /locus_tag="ACHKUB_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO6654649.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17083..18249)
                     /gene="dacD"
                     /locus_tag="ACHKUB_001618"
     CDS             complement(17083..18249)
                     /gene="dacD"
                     /locus_tag="ACHKUB_001618"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO6654650.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKNLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRVKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKV"
     gene            18458..19885
                     /gene="sbcB"
                     /locus_tag="ACHKUB_001619"
     CDS             18458..19885
                     /gene="sbcB"
                     /locus_tag="ACHKUB_001619"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO6654651.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(19928..20155)
                     /gene="tsuB"
                     /locus_tag="ACHKUB_001620"
     CDS             complement(19928..20155)
                     /gene="tsuB"
                     /locus_tag="ACHKUB_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO6654652.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20169..21227)
                     /gene="tsuA"
                     /locus_tag="ACHKUB_001621"
     CDS             complement(20169..21227)
                     /gene="tsuA"
                     /locus_tag="ACHKUB_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO6654653.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAILVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(21406..22764)
                     /gene="plaP"
                     /locus_tag="ACHKUB_001622"
     CDS             complement(21406..22764)
                     /gene="plaP"
                     /locus_tag="ACHKUB_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO6654654.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(22754..22816)
                     /gene="yoeI"
                     /locus_tag="ACHKUB_001623"
     CDS             complement(22754..22816)
                     /gene="yoeI"
                     /locus_tag="ACHKUB_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO6654655.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(23031..23960)
                     /gene="yeeY"
                     /locus_tag="ACHKUB_001624"
     CDS             complement(23031..23960)
                     /gene="yeeY"
                     /locus_tag="ACHKUB_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6654656.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(24006..24830)
                     /gene="yeeZ"
                     /locus_tag="ACHKUB_001625"
     CDS             complement(24006..24830)
                     /gene="yeeZ"
                     /locus_tag="ACHKUB_001625"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6654657.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(24913..25167)
                     /gene="yoeB"
                     /locus_tag="ACHKUB_001626"
     CDS             complement(24913..25167)
                     /gene="yoeB"
                     /locus_tag="ACHKUB_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO6654658.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(25164..25415)
                     /gene="yefM"
                     /locus_tag="ACHKUB_001627"
     CDS             complement(25164..25415)
                     /gene="yefM"
                     /locus_tag="ACHKUB_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO6654659.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            25698..25748
                     /gene="hisL"
                     /locus_tag="ACHKUB_001628"
     CDS             25698..25748
                     /gene="hisL"
                     /locus_tag="ACHKUB_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO6654660.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    25724..25847
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            25894..26793
                     /gene="hisG"
                     /locus_tag="ACHKUB_001629"
     CDS             25894..26793
                     /gene="hisG"
                     /locus_tag="ACHKUB_001629"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO6654661.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            26799..28103
                     /gene="hisD"
                     /locus_tag="ACHKUB_001630"
     CDS             26799..28103
                     /gene="hisD"
                     /locus_tag="ACHKUB_001630"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO6654662.1"
                     /translation="MSFNTIIDWNNCTAEQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            28100..29170
                     /gene="hisC"
                     /locus_tag="ACHKUB_001631"
     CDS             28100..29170
                     /gene="hisC"
                     /locus_tag="ACHKUB_001631"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO6654663.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALNPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            29170..30237
                     /gene="hisB"
                     /locus_tag="ACHKUB_001632"
     CDS             29170..30237
                     /gene="hisB"
                     /locus_tag="ACHKUB_001632"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO6654664.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            30237..30827
                     /gene="hisH"
                     /locus_tag="ACHKUB_001633"
     CDS             30237..30827
                     /gene="hisH"
                     /locus_tag="ACHKUB_001633"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO6654665.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            30827..31564
                     /gene="hisA"
                     /locus_tag="ACHKUB_001634"
     CDS             30827..31564
                     /gene="hisA"
                     /locus_tag="ACHKUB_001634"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO6654666.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            31546..32322
                     /gene="hisF"
                     /locus_tag="ACHKUB_001635"
     CDS             31546..32322
                     /gene="hisF"
                     /locus_tag="ACHKUB_001635"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO6654667.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALAEPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            32316..32927
                     /gene="hisIE"
                     /locus_tag="ACHKUB_001636"
     CDS             32316..32927
                     /gene="hisIE"
                     /locus_tag="ACHKUB_001636"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO6654668.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVANIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(33024..34001)
                     /gene="wzzB"
                     /locus_tag="ACHKUB_001637"
     CDS             complement(33024..34001)
                     /gene="wzzB"
                     /locus_tag="ACHKUB_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO6654669.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(34148..35314)
                     /gene="ugd"
                     /locus_tag="ACHKUB_001638"
     CDS             complement(34148..35314)
                     /gene="ugd"
                     /locus_tag="ACHKUB_001638"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO6654670.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(35564..36970)
                     /gene="gndA"
                     /locus_tag="ACHKUB_001639"
     CDS             complement(35564..36970)
                     /gene="gndA"
                     /locus_tag="ACHKUB_001639"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO6654671.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVADDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(37134..>37357)
                     /locus_tag="ACHKUB_001640"
     CDS             complement(37134..>37357)
                     /locus_tag="ACHKUB_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO6654672.1"
                     /translation="SVLDVINTFQKISGIAINYIFSQRRSGDIAECWSDPSLALKELQ
                     WQAKLSLEDMLRDAWNWQINNPDGYNIHD"
BASE COUNT         9570 a         9645 c         9312 g         8830 t
ORIGIN      
        1 gttctctatt aatttttctc attagccgat taagtcagcc attctttttc ctcgcttttt
       61 tttatagtca tatcgtcaaa aaaatccagg cgaggtcttt atgcaacaag aagcgttagg
      121 aatggtagaa accaaaggct taactgccgc catagaggcc gcagacgcca tggtgaagtc
      181 ggcaaatgtt ttgctggtcg gctacgaaaa aataggttcc gggctggtga ccgtcatcgt
      241 tcgtggtgat gtcggggcgg taaaagcggc aacagacgca ggtgccgtag ctgcgcgtaa
      301 cgtcggtgaa gtaaaagccg tacacgttat tccccgccca cataccgatg tcgaaaaaat
      361 cttaccaaag ggaattaacc catgagcagc aatgaactgg tcgaacagat catggcgcag
      421 gtcatcgctc gcgtagcgac gccagagcat gaggttctcc cggaaaccac tcatcaaaca
      481 cgagagacgg ctatggcaga aaaaagctgc acgttaacgg aatttgttgg caccgctatt
      541 ggcgacactg taggtctggt cattgcgaac gttgatagcg ccctgttgga cgcgatgaaa
      601 cttgaaaaac gctatcgttc cattggcatt ctgggtgcgc gtattggcgc agggccacac
      661 attatggccg ctgatgaagc ggtaaaagcc accaacactg aagttattag cattgaactt
      721 cctcgggata ctaaaggcgg tgccggacat ggctcattga tcctcttagg tggcaacgat
      781 gtttctgacg tgaaacgcgg tattgaagtc gccctgaaag aactcgatcg gacctttggc
      841 gatgtctatg ccaacgaagc cggacatctt gaactgcaat ataccgcccg cgccagctat
      901 gcactggaaa aagcgtttgg tgcaccacag ggacgggctt gcggcgttat tgtcggcgct
      961 ccggctgctg tgggtgttct gatggccgac accgcgctga agtccgccaa tgtggatgtt
     1021 gtggcataca gctccccagc tcaaggcacc agctacagca atgaagtcat tctgattatc
     1081 tcgggtgact ccggcgcagt ccgccaggcg gtcatctccg cacgcgaaat cggtaaaacc
     1141 gtgctttcga ctctcggcga tacaccgaaa aacgatcgcc cttcctacat ctgaattctc
     1201 tgaggcagat ttatgagatc gaaaagattt gaagcactgg caaaacgccc ggtcaaccag
     1261 gacggtttcg ttaaagagtg gattgaagaa ggctttattg cgatggaaag cccgaacgat
     1321 cctaaaccgt caatcaaaat tgttaacggc gtcgttaccg aactggatgg aaaacccgcc
     1381 agccagtttg acctgatcga ccactttatc gcccgctacg gcatcaatct cgcgcgcgcc
     1441 gaagaagtca tcgcgatgga ttcggtaaaa cttgccaata tgttgtgcga tccaaatgtc
     1501 aaacgtagcg atatcgttcc ccttaccaca gcaatgacgc ctgcaaaaat cgtcgaagtg
     1561 gtttcgcaga tgaacgtggt agagatgatg atggcgatgc agaaaatgcg cgcccgtcga
     1621 acaccatcac aacaggctca tgtcaccaac gtcaaagata acccagtaca aattgccgcc
     1681 gacgcagctg aaggcgcatg gcgcgggttt gatgaacagg aaaccaccgt tgccgttgct
     1741 cgctatgcac cgttcaacgc catcgccctg ctggtcggct cacaggtggg acgtccgggc
     1801 gtcttgaccc agtgttcact ggaagaagcc accgaactga aactcggtat gttaggccac
     1861 acctgttacg ccgaaacaat ttctgtctat ggtacagaac ctgttttcac cgatggtgat
     1921 gataccccgt ggtcaaaagg gtttctcgcg tcatcctatg cttctcgtgg cttaaaaatg
     1981 cgctttactt ccggctccgg ctctgaagta cagatgggtt acgcggaagg caaatcaatg
     2041 ctttacctcg aggctcgttg catttacatc accaaagccg caggcgttca gggtctgcag
     2101 aatggttcgg taagctgcat cggtgttcca tcagctgtgc catcaggcat ccgcgcagta
     2161 ttagcggaaa acttaatttg ctcatcgctg gatctggaat gcgcctccag taatgaccag
     2221 acctttaccc actcggatat gcgccgtacc gcacgtttcc tgatgcaatt tctgccaggc
     2281 accgacttta tctcttccgg tttttccgct gtgccgaact acgacaacat gtttgctggt
     2341 tcaaacgaag atgctgaaga cttcgatgat tacaacgtca ttcagcgtga cctgaaggtt
     2401 gatggtgggc tgcgaccagt acgcgaagaa gatgttatcg ctatccgcaa taaagccgct
     2461 cgcgctttac aggctgtctt tgccggtatg ggactaccgc ctattaccga tgaagaagtc
     2521 gaagcggcaa cctatgccca cggttcaaaa gatatgcctg agcgcaatat tgtggaagac
     2581 atcaagttcg cccaggagat tatcaataaa aaccggaaca gcctggaagt ggtgaaagcc
     2641 ctggcgcaag gcggttttac cgatgtcgcc caggacatgc tcaacatgca aaaagcaaaa
     2701 ctgaccggcg attatctcca tacctcagcc atcatcgtcg atgacggaca agtgctctct
     2761 gcggtcaatg acgtcaatga ttacgccgga ccggctacag gttaccgcct gcaaggggaa
     2821 cgctgggaag agatcaaaaa tattcccggt gcacttgatc ccaacgaaat tgactaaggg
     2881 tgagaaatgg aaattaacga aaaattactg cgtcagatta tcgaagacgt gctggcagaa
     2941 atgcaaccca gcgacaagtc cgtttcattt cgcgcacctg tctctgcaac tgtctcttcc
     3001 gcgccagata ccggaaattt tctgaccgaa attggcgaag cccaacaagg cactcagcaa
     3061 gatgaagtca ttattgctgt aggcccggcg ttcggcctgg cgcaaaccgt caatatcatc
     3121 ggtattccgc ataaaaacat cttgcgcgaa gtgattgccg gtatcgaaga agaaggcatt
     3181 aaagcccgtg tgattcgctg ctttaaatcg tccgatgtcg cattcgtagc agtcgaaggc
     3241 gatcgtctta gtggttcagg tattgctatc ggtattcagt cgaaaggcac aacggttatc
     3301 caccagcagg gacttcctcc cttgtctaac ctggaacttt tcccacaagc gcctttgttg
     3361 acgctggata cttatcgtca gattggcaaa aacgccgcgc gttatgcgaa gcgcgagtct
     3421 ccacaaccgg tgccgaccct caacgatcaa atggcgcggc cgaaatacca ggccaaatct
     3481 gccattttgc atattaaaga gacgaagtac gtggtcactg gcaaaaaacc gcaagaactg
     3541 cgcgtgacgt tttaacaaag gaaaccttca tgaataccga tgcaattgag tcgatggtca
     3601 gggacgtgtt gaaccgcatg aacagtttac aggatgcagc tcctgtttct gcggtcccca
     3661 acgcatcaat actgagcgcc aaagtgacgg attacccgct ggcaaataaa catccggaat
     3721 gggttaaaac agcgaccaat aaaacgctcg acgatttcac tctggaaaat gtgctaagcg
     3781 ataacgtcac cgccctggat atgcgcatta cccctgaaac attgcgtatt caggctgcga
     3841 ttgccagaga tgctggtcgc gatcgtctgg cgatgaattt tgaacgcgcc gctgagctta
     3901 cctcagtacc agatgatcgc atcctcgaaa tctacaatgc gttacgccct tatcgttcaa
     3961 ctaagcaaga acttattgcc attgctgacg atcttgaaca gcgttatcag gccaaaattt
     4021 gtgctgcatt tgtccgtgaa gctgcggaac tttatgtcga gcgcaaaaaa ctgaaaggcg
     4081 acgattaagg acgaactatg cgatatatag caggcattga tatcggcaac tcatcaacag
     4141 aggttgcgct ggcaacgctc agtgcaactg gagagttgtc gttcgtgagt agcgccctgg
     4201 cggaaaccac cggaattaaa ggtacgttgc gtaacgtcca tggtattcag gaggcgctcg
     4261 cacaggcaac gaaaaaagtc ggcatcaatg ttagcgatat ttcgcttatt cgtatcaacg
     4321 aagcaactcc ggtcattggt gatgtggcga tggaaaccat cactgaaact atcatcaccg
     4381 aatcaaccat gatcggtcac aacccgaaaa ctccgggcgg agtggggctg ggcgtagggc
     4441 tcaccatcac tccgcaagag ttattaacac gcccggcgga cacaccttac atcctggtgg
     4501 tgtcatcggc atttgatttt gccgatatcg cgacgatgat aaacgcctcc gtacgcgccg
     4561 gatatcagct gactggcgtt attctgcaac gcgacgatgg cgtactggtc aataaccgcc
     4621 tggaaattcc tttacctatt gtggatgaag tcctctacat cgatcgtatt ccgctcggca
     4681 tgctggcagc aattgaagtt gccgttcccg ggaaagttat cgaaaccctg tctaacccgt
     4741 atgggattgc caccgtattc gccctcaatg ctgaagagac taaaaatatt gttcctgtcg
     4801 cgcgggcgct aatcggcaac cgttcagcag tggtagtgaa aacgccctct ggcgacgtta
     4861 aagcccgttc gatacccgcc ggaaatatcg aactgctttc tgccgggcgc acaacgcgcg
     4921 ttgatgtagc tgctggtgcc gatgccatta tgaaagcggt cggtgagtgc ccaaaactgg
     4981 aaaacgtgac gggagaacca ggcaccaaca tcggcggaat gctggaacat gttcgccaga
     5041 ccatggcgga actgaccaac aaacccagca acgaaatttt tattcaggat ttgctggcga
     5101 tagatacctc tgtccccgtc agcgtaaccg gtggtctggc aggtgaattt tcactggaac
     5161 aggctgtggg tattgcctct atggtgaaat ccgaccgtct gcaaatggcg atgatcgcca
     5221 gtgaaattaa gcaaaaactg catgttgatg ttcaggtcgg aggcgcagaa gcagaagccg
     5281 ccattcaggg ggccttgact acccctggaa ccacacgtcc actggcaatt ctggacttag
     5341 gtgcaggctc taccgatgcc tccatcatca atcagagtgg ggaaatcgtt gccactcacc
     5401 ttgcgggagc gggcgacatg gtaacgatga ttatcgctcg tgagctgggc ttaaatgatc
     5461 gttatctggc agaagaaatc aaaaaatacc cgttggcaaa agtagaaagc ctgttccatt
     5521 tacgccatga agacggaagc gttcagttct tccctactcc gctgtcccct catgtatttg
     5581 cccgcgtttg tgtggtgaaa cctgacgaac tggtgcctat tcccggcgat ttaacactgg
     5641 aaaaagtacg tgctgttcgc cgcagtgcga aagagcgcgt cttcgtaacc aatgccctgc
     5701 gcgcgctacg tcaggtaagt ccaggcggaa atattcgcga tattccgttc gtcgttctgg
     5761 tgggcggttc ttccctggat ttcgaagtcc cgcagttagt caccgatgca ttggcgcatt
     5821 atcgattggt cgccggacgg gggaatatcc gtggtagtga aggcccgcgc aatgcggtag
     5881 ctaccgggct actgatttcc tggcacaggg agttggctca tggaaagtaa agaacatacg
     5941 cccgctattg tcgttacgga aataggtgac tgtatcagta catggaatga ggtgcttctt
     6001 ggcattgaag aggaaggcat cccctttcgc attcaacata tcccgtcagg tgaagtcatc
     6061 gatagcgcct ggcaggctgc tcgtaagtcc cccctgttgg ttggcatagc ctgcgaccgg
     6121 gaaaagctga ttgtgcatta caaaaactta ccggcatcag cgccactgtt tacgctgatg
     6181 tatcaacaag ataaccatac ccggcgcagc atcggcaata acgctgcccg cctggtaaaa
     6241 ggcattccct tcagggaatg ccactcataa tccacaggag aaacgctgta tgaacaacgc
     6301 actaggactg gttgaaacaa aagggttagt tggcgcaatt gaagctgcag atgccatggt
     6361 taaatccgcc aacgtgcaac tggttggcta tgaaaagatt ggttcgggtc tgattacagt
     6421 gatggtccgt ggcgatgttg gcgcagtaaa agccgccgtt gacgcaggaa gcgccgccgc
     6481 aagcgccgtt ggcgaagtga aatcctgcca tgtcatcccg cgcccacaca gtgatgttga
     6541 ggctatttta cctaaatcgg cataacccca tttactaagg agcacagcgt gaagcaatca
     6601 ctgggattac ttgaagtcag tggtctggct ctcgccatca gttgcgcaga cgctatggca
     6661 aaagccgcgt ccatcacgct ggttggcctt gagaagacca atggctcagg ctggacgctc
     6721 atcaaaatca ccggtgacgt cgcctcggtt aatgcggcga tagcgacggg agccagtttc
     6781 gccggacagc gaaatggcct tgtcgcacac aaagtgattg cccgcccggg agacggcatt
     6841 ctgccccaca caactgaagc attgaccgtg acggttgaaa caagcaaaac accagcaaca
     6901 accgtcacct cagagattat cagttgtaat ttgtgtcttg acccggcatg tcagcggcaa
     6961 aaaggtgagc cccgctcact ttgcctgtac gccaataagc gaggggagta gcagtggata
     7021 aacaaacctt gcaggaaaca gtgaaccaga ttctggatga gatgcgcgag cgccctatcc
     7081 cactgggcgt ttccagtcgc catatacatc tttcaacagc ggattatttg cgtctttttc
     7141 ctgaacaacc gattagcgag aaaaagccgt taatgcaacc tggccaatac gctgcggaac
     7201 aggttgttac gctggaggga ccaaaaggca aattgcacaa cgttcgcttg ttggggccgc
     7261 tacgcaatgc aagtcaggtg gaaatttccc ggacggacgc cagaacgtta gggatcaccg
     7321 ccccgctacg catgtcaggc gatctcagcg gcactccggg gattcgtttg ataagcccat
     7381 ttgcggaact ggagatcaca tctggcgtta ttgtcgcgca acggcatatt catatgtctc
     7441 cgctggatgc cctgatttat ggcgttacgc atggcgataa agtggcggtt gccattgaag
     7501 gtgatgagcg acgactgata ttcgaaaacg tcgctgttcg cgtctctccg gatatgcgtc
     7561 ttgaaatgca tattgatacc gatgaggcca acgccgcagg cgctgatagc cctgacgttt
     7621 ttgccacact ggtgtcagtg ccatgaataa catgctgatg caacgtattg ttgacgaagt
     7681 ggtttttcgc cttaaacaac gtgcgggaaa aacactcgtg ctgactgttt ttcaactgcg
     7741 agacgccagc gttcaagaga gcgttcatca gtatgcgtca ttacaaatcc gatatgtcga
     7801 tctgccgctg ctgcgacaac ttgcggagaa cgaaacgtca gatcgagcag cgattcagat
     7861 acacgaagcg ttagcctggg gattgcacat tcaattgtcg ttgcagcgtc atttcctgaa
     7921 cgccattgaa ttgaagactc tcgcccgtct accgctaagc tggtgcgacg aacaagggca
     7981 accgatctac cttcatcgtg atcgactttt aagttatgcc gatatcgcac agttaagctc
     8041 gggaatactg gttttacagc ggaaatgttg tgtgaccgca ctggctcgtg aagcggcaat
     8101 tacccgaaat attcagttaa tcaggcagga gtaacttatg catctggctc gagtcactgg
     8161 cgcggtagta tccacgcaaa aatcaccttc tctgatcggg aaaaaactgc tgctggttcg
     8221 ccggatcagt gctgacggcg aactgcctct cacgccatta accggcgacg aagttgccgt
     8281 tgattcagtc ggcgcaggcg ttggtgagtt ggtactcctc agcagtggtt ccagcgccag
     8341 acatgtgttt tcaggcccta acgaggccat cgacctggca gtggtcggca ttgtcgatac
     8401 gctttcgcgc taaggagcta aaagatggca atttataccc gaacaggtga ttcaggaagt
     8461 acgtctttat ttaccggaca gcgcgtcagc aaaactcatc tacgtgtcga aacctatggc
     8521 acgctggatg aactgaacgc gacactcagt ctgtgttatt gcgccaccgc catcgaaagc
     8581 catcgcatct tactggaagc cattcaacaa cagatatttt ggtttagcgc cgaactcgcc
     8641 agtgaaagtg aacagccgtc agcgcaacag cgttacatcg gtacggaaga aattgctgcg
     8701 ctggaaaacg cgatcgatag cgcaatgaac gccgtccccc cagttcacag cttcattctg
     8761 cctggtcgat gtgaagccgc gagccgcatg catttcgccc gcacagtggc tcgacgggct
     8821 gaaagacgtc tggttgaact gacaacggaa accaccgtcc ggaatgtttt gctgcactat
     8881 atcaaccgac tttccgattg tctgtatgcc ctcgcccgcg tggaagacaa cgttgctcat
     8941 caaaacctga tgattcagga aatcacaaaa cgttatcacg aagccaacca cataccagca
     9001 ttgaaggaac gcacaatgcc gctcactttt caggatcttc accagctcat tcgtagtgcg
     9061 gcaatgcgcg cggatgaact gcatattccc gttgtcatca gcattgttga tgccaacgga
     9121 acagaaagtg ttacctagcg gatgcctgat gccctgttgg tcagcagtga actggcaccg
     9181 aaaaaagcct ggaccgccgt ggcgatgaaa acggcaaccc acaaacttgc tgataccgtg
     9241 caacccggcg cgccgcttta cgggctcgaa agccatatgc aaggcaaagt cgtcactttt
     9301 ggtggcggct tccccctctg gcgtgacgga aaattgcttg gcgggcttgg catcagcggc
     9361 ggtagcgttg aacaagacat ggatattgct caaagcgcaa tggcggcaat taacgtggga
     9421 gtaaaccaat gaataactct gaattagaaa cactgatccg caatattctg cgcgagcaac
     9481 ttgtccccgc tacaccacga acgcaaagaa acgctatttt ccagactgtt gatgaagcgg
     9541 tctgcgcagc acaccaggcc tttttacgct ttcaacagtg tccgcttaaa acccgtagcg
     9601 ccattatcag cgccattcgc gaagaattaa tgccccgcct taccgaactg gcagaagaaa
     9661 gcgccaaaga aacggggatg ggaaataaag aagataagta cctaaaaaac aaagcggcac
     9721 ttgataacac gccaggaatt gaagatttaa ccaccacagc actaaccggt gatggtggca
     9781 tggttttgtt tgagtactct ccgtttgggg tgattggctc ggtcacgcca agcaccaacc
     9841 ccacggaaac catcattaac aacagcatca gcatgctggc agcaggtaat agcgtctact
     9901 tcagtccaca cccgggtgcc aaaaacgttt cgttaaaact gattaccaca atcgaagaca
     9961 tcgcgtttcg ctgtagtggc attcataacc tgatcgttac cgtatctgaa ccgacctttg
    10021 ctgccaccga acagatgatg gcgcacccta aaatcagcgt tctggcgatt accggcggtc
    10081 cggggatcgt cgccatggga atgaaaagtg gcaagaaagt gattggtgcc ggagcgggta
    10141 accctccgtg catcgtcgat gaaacggcgg atattgtgaa agccgccgag gacatcatca
    10201 acggcgcttc atttgattac aacctgccgt gtattgctga aaaaagcctg attgtcgtgg
    10261 catcagtggc agattattta attcagcaga tgcaaagttt tggcgcgctt cttcttaact
    10321 atgagcaaac tgagaaattg cgtgcaattt gcctgccaga cggtagcgcc aacaaaaaac
    10381 tggttgggaa aagcccatca gccttgctgg aagcggcagg cctccctttg cccgcaaaag
    10441 cgccgcgcct actgattgct gttgtcgacg ccaatgatag ttgggttact tgtgaacaac
    10501 tgatgccaat gctgcccatt gtgaaggtca acgattttga tagcgctctc accctggcgc
    10561 tgaaagtgga agacggcttg caccacacgg caattatgca ctcgcagaac gtctcacggc
    10621 tcaaccttgc cgcacgcgtt atgcaaacct caatctttgt caaaaacggc ccctcctggg
    10681 cgggtattgg cgtcggcggc gaaggcttta cgacctttac cattgcaaca ccgaccggag
    10741 aagggacaac gtcagcacgg acttttgccc gctcacgacg ctgcgtgtta accagcggct
    10801 tttcaattcg ctagagaaac ggaaatgaat actttttcac tgcagacgcg tttatacagc
    10861 ggaccgggaa gtctcaaggt aattagccgt ttctctcata aacacatctg gattatttgc
    10921 gacgtctttc tggcacgctc accactcatt aacacgttgc atcaggcgct gcccgacagt
    10981 aatcgtctta gtatttttag cgaaataacg cccgacccaa ctatccagac cgtggtgaaa
    11041 ggcattgcgc aaatgcagac tttacgcccg gatgtggtta tcggttttgg cggcggttct
    11101 gccctggacg ccgcaaaagc gattgtctgg ttcggtcgcc agtgtggcat tgaaattgaa
    11161 acctgcgtag ccattcctac caccagcggt accggttccg aagtgaccag tgcttgtgtc
    11221 atcagcgatc ctgagaaagg gattaaatat ccccttttcg ataatgcact ttatccggat
    11281 atcgcgatcc ttgatccatc tctgatcgtc agcgtgccac cggcgataac ggccaatacc
    11341 gggatggatg ttttaaccca tgcgcttgaa gcttatgttt ctcctcgcgc cagcgacttc
    11401 acggatgcac tggtcgaaaa agccgtgcaa attgtttttc aatacctgcc cactgcagtg
    11461 aaaaaaggcg attgtcttgc cacccgtggc aaaatgcata atgcatcgac gcttgcagga
    11521 atggccttta gccaggccgg actcggttta aaccatgcaa ttgctcatca actcggcgga
    11581 cagtttcatc tgcagcatgg actggcgaat gcgctcctgc tcacggcagt tatccgcttt
    11641 aatgctggcg atccgcgcac agccaaacgc tatgcccggc tggcgaaaac gtgccattta
    11701 tgcccggaca acgccaatga caccgccagc ctgaatgcac ttattcaaca tattgagcag
    11761 cttaaaacaa cctgtacgct gccgactctt gctaatgcgc tgaaagaaaa aaaagcagaa
    11821 tggtcaatac gcataccaga tatggttcag gcggcactgg cggatgcaac gttgcgtacc
    11881 aacccgcgtg cggctgatgc ctccgcaatt gcagaactgc tcgaggagtt gttatgaacg
    11941 tcaagttaac ctcctgcgac gaccagacca tccgcgagcg agtacaagca gcaggtgtcg
    12001 tgggtgctgg cggcgcaggt tttccaaccc atattaagct acaagcgcgt gttgacactt
    12061 tcctggtgaa tgcggcggaa tgcgaaccca tgttgaaagt ggatcaacag ttaatggcgc
    12121 agcaagccag ccgcctgctt cgcggtgttc actatgcgat gaaagccacc ggggccagca
    12181 gcggaatcat cgcgctgaaa gagaaatatc aacgggcaat aaacgcactc acaccactgt
    12241 taccaccgga tatccgcctg catattttgc ccgatgttta cccggcagga gacgaagtgc
    12301 tgaccatctg gatggcaact ggtcgccgcg ttccacccgc tgcattaccc gttagcgtcg
    12361 gtgtggtggt caataatgtt caaaccgtac ttaatattac cagagccgtc gagcaacagt
    12421 atccagtgac gcgccgcaca ctcaccgtta atggtgcagt ggcgaaacca ataacggtaa
    12481 ccgtccccat cggcatgtct ttacgtgaag tcctggcgct ggcgggcggc gcaacagtgg
    12541 atgctcctgg ttttatcaat ggcgggccga tgatgggaag tttgatcaca tcgctcgaca
    12601 caccagtcag caaaaccact ggcggcttgc tggttttacc caactctcat tcactgatcc
    12661 aacgtcgatt gcagaatgac cgcagcgtac ttgccgtcgc gaaaactgtt tgtgagcaat
    12721 gccgcttgtg taccgatctc tgcccacgtc atttaatcgg tcacgaactg gcaccccatt
    12781 tattagtacg tgcggttaac taccaacagc tcgccacgcc gcaactgtta ctgacagcgc
    12841 tcacctgttc agagtgcaac gtctgcgcca gtgtcgcctg tccggtagga atttcaccaa
    12901 tgcgcataaa ccgcctgctt aagcaggaat tacgcgcaca aaatctccgc tacgaaggta
    12961 ccttaaatcc tgcagatccc atggcaaatt atcgactgat tcccgtaaaa cagctggtaa
    13021 ccagacttgg cttaaccccg tggtatcagg atgcgccatt gtctgaacag gtgcctcagc
    13081 ccgaaaaagt gacgttattg ttacgccagc acatcggtgc cagcgcaatt gcgtgtgtac
    13141 aaaaaggcga tcgcgtcgtt catgggcaat gtgtaggtca aattccacac ggcacattag
    13201 gtgcccccat tcatgccagt attgatggca tggtcagcga tgtgacagaa aacgcgatta
    13261 cgcttgtgag aggttaacca tgtcgaaagc ggtaggtatt ttagaactta gcagtatcgc
    13321 gaaagggatg gaaaccggcg acgccatgct caaaagcgcc aacgttgagt tactggtcag
    13381 caaaacgcta tgccccggaa aatttctgct gatgctcggt ggcgatgtgg gcgcggtgca
    13441 acaggccatc aacagcggag cgtcccttgc tggtgaaatg ctggtcgaca gtcttgtcct
    13501 gccgaatatt catcccagcc tccttactgc aatcagcgga cttaaccatg ttgaacagca
    13561 ccgggctgtc ggcgtagtcg aaacctggag tgtcgccgcc tgcattgacg ctgccgattg
    13621 tgctgtcaaa gctgcgaatg tcattctcgt tcgcgtacat atggcttttg gcattggcgg
    13681 taaatgctac atggtggttg ctggcgatat ttcagatgtt aataacgcgg tctctgtcgc
    13741 cagcgagcgt gcgggggaaa aaggtttgtt ggtctatcgg tcggtgattg cccgtccaca
    13801 tgaagccatg tggcgtcaga tggtggaggg ataatgacaa ctcagcacac aactgaacgg
    13861 atgattcagg aatatgtccc tggtaagcag gtcacgctgg cacatgtcat cgccaatcca
    13921 gggaaggatc tgtttaagaa attgggttta ccagacacca tttccgctat tggtattctg
    13981 acaattaccc ccagcgaagc atctatcatc gcatgcgata tcgccacaaa atcaggcgct
    14041 gtagagatcg gttttcttga tcgttttacc ggtgccgtag tgctgactgg tgatgtttct
    14101 gcgattgaat atgcacttag ccaggttacc cgtacgctgg gtgaaatgat gcgctttacc
    14161 gcctgcccca ttacgcggac ctgacgttat gcaacgtatg atgctcatcg gccccagcca
    14221 gtgcggaaaa acgtcactca cgcaatgtct gcgtggcgaa aatattcatt atcagaaaac
    14281 gcaggcaatc gtctggtccc cgaccaccat tgatacacca ggcgaatacc tcgaaaaccg
    14341 cagcctctac agtgcactgt tggtcagtgc ctgcgaagcg gacattatcg cgctggtgct
    14401 taatgcggac gcaccgtggt cacctttttc gccaggcttt acctcgtcaa tgaatcgccc
    14461 ggtcattgga gtagtaacca aagcagattt agccaacctg gaacaaatat ctttggtgaa
    14521 atgttggtta cgggaagctg gagcgcacaa cgtgttagta accagtgcgg ttaataacaa
    14581 tggagtggca gaacttttag ccttgctgca tatagaagaa ggctgtcgct aatttcgaca
    14641 ggttctcaga tgggaatgat gacggtgata cacagaaaag aatcaatgaa caatgagggt
    14701 tgccaggcaa ccctcattga ataaaacagg aattacttcg cttcgccgtt tttcatttcg
    14761 cccatagctt tcagcttttt ggagatatca cggcgttctt tggagagctc ggcatttttg
    14821 atgatgtaat cgtcaacgcg atcttcatag tcacctttca tgctagcgat gatgccctgg
    14881 attgcttcaa cgctcatccc cggcttgatg taatcgctca ggttgtccag cagcaggacg
    14941 cgcttctggt tgtcacggat ctttttctca acgtcctgaa tttcacgttg cagtttgttc
    15001 ttacgacgga acagacgaac aaattccagt acgtcctgga atgaaggctt ggtagtttcc
    15061 atttttatac ccctgataat gtgagagtcg gattcgttta agcaaccgct attcgttagg
    15121 gccaacatta ctcatagctg ccgcagatga caatgctttt atcccttttc actatcatac
    15181 cctttatcct tgctgaatcg aagcagcagc aagatgattc tgaagttcag gaattatttg
    15241 cgcaaggccc ggcaaatcag attcgatagc aactcaagct gatgtgccgt ttccatgttc
    15301 agccacacat aaccatagat tggtgtttcc acaaccttca ggtcatggtg gttattgagc
    15361 aactgacgca gctcttccac agcatcgttc aatttttccg tattggcaaa aaccggctgc
    15421 ggattacctt cgtacagcgc atgaacaagg ctcagcagta tttgctgcat catgcgctgg
    15481 gtatcacgaa gtttttgtgc gttcaataac acgaaatggc tggggcgcgt ggcccagtac
    15541 gcattgattt gcaactccag catacaaacc agattgcggt taatggtctg gataccttca
    15601 tatatcgatt ttggaatacg ggtttctttg ctggcgggcg caatcagccc acgcattttc
    15661 acagcatcgg tcaggagttt ttgtagatgg ctttccagac gtgggcgttc gagtaagttc
    15721 ggtgagaatg ctgattgata gacccgatta tactcggtca gacttttcgc cagttgaatg
    15781 cgccagtgga tgaacgcccg ttgtggccag ataccggtaa acaacattgc cagtaaagag
    15841 ccgaggatca catcgccgct tcgccataac gccgtatcaa tttcacctgt cggggaaccc
    15901 acaacaattg ccagcgtcac cccaatcaat aatccttgat acggtttctt gcccagcgcc
    15961 agccaaccgc aaagaaacat agccgccgcg caccagacta acatcagcgg taacgagatt
    16021 aactccagtt gcagagcgat aagacctaat atcgaaccca acaccgtacc gccaatacgt
    16081 tcgaaggcac gagggacaac gttaccccag aacgagattg gccccataat caccaccatg
    16141 gtgaccagcg gccaggtact ttccgggata gtaaacaggc ggataatgag aaaagtgagc
    16201 aggaatgcca gcgcgacccg agtaccatgc acaatgcggt aatggcggta tacccggatt
    16261 tcaaacgggc ttaatgactt atcggcacgc acgcagaaac tccgtaatga agattaaaag
    16321 cgctcataac acatctgtcg gccagaaggc gcgcctctga gttatccgac ggacataccg
    16381 ggaacagtgt aatttcccgg caggagcgaa aaaattgaaa ggcgcatctt actcttttcg
    16441 ctttcaaaaa aaagagtggt catcgcgtta acacaccgcc ctgagatgaa ttagtgatgt
    16501 tttggctgca ccgcaacata catttcgata tcccagtacc catcttccgc gccattgttc
    16561 aaataaacct cgaagcaggg ctttggtaac atttcataag cactgtcctg caagaggcta
    16621 ttaaagaact gataccaggg tttagcaaaa tcatcaccga ctacacgagc caccgccacc
    16681 gcatactgac cacctgaaat ttctgtcaga atgacgccct cactgttttc ggggagcgta
    16741 aagttattcg gaaccgtcac gacggtatcg cagcgtaatt tttcggcggg tgtttcgtct
    16801 ggattgtcgt aatagacagc aacccactcc ttcggcacaa tatttttgtt atctacccac
    16861 atcatcaact gctcaaaacc tttctttacc gtctgctccc acgggccaac gagatggaaa
    16921 cctgcaacgg tacgtttatc ttcctgctta atctcgtagt tcatgacgcc tccattaata
    16981 ctgtttttat atacagtata gttgcaatat taaaaccaca aggaatgagt gttgattatg
    17041 cgagcagact cgcactcctg ccagtctgct gcaaaagaaa ggtcagacct tatggtggaa
    17101 ataatcactc aggcgagaaa acatgctgcc ttccccgaca gattccaggg taaccagcgg
    17161 ccagtgcgcc acctgtttat cacggtcgta aagttcaatt tcccctaccc gctgatgggc
    17221 gctaattggc gcggtgagct ctttaccatc aagggtatat ttagctttga tatgtggaat
    17281 ttcggctttc ggtagcacca tccagaactc ttgttccgtt cccagggcga tattttcttt
    17341 atcgccatac cagatgcgtt ccgtaccgac ctttttcaca cggtgcaaaa tttgcaccgt
    17401 agtaaagttt tgttgccccc aacgcagtaa ttttcttgcc tcttcctcac gaccttttgc
    17461 actgtcagcc cccattacca ctgcaatgag acgacgctgc ccatctacag ccgaagcaat
    17521 gagattaaac ccggcaccag aagtatgacc cgttttcagg ccgtcaacat tcatggtttt
    17581 atcccacaat aacccgttac ggttttgctg agtgataccg ttccaggtga ggtttttctc
    17641 actgtacata tgataaaact cgggctcgcc gtggatgata gcgcgagaaa gcacagctaa
    17701 atcataagcc gagctatgct ggcctggtgc atccagacca tgcactgttt caaaatgcgt
    17761 atccttgaga tgcagcttct cggcatagtt gttcatcatt tcaacaaact gccgttgccc
    17821 accggcaata tagtcagcca gtgcaacaca agcgtcattt ccggaatcca caattaaacc
    17881 acggcttaaa tcacgtaccg atacgcgatc gccctctttc aaaaacatca gtgaagaacc
    17941 gacaaacacc ggattatctt tcgcccacgc atcgcgcccc acggtgacaa tatcgtctgg
    18001 cgtaatgcga tgactatcga tagcgcgatc cacgacataa cccgtcatca gctttgtcag
    18061 gctggcggga ttgcgctgtt gatgctcatt acccgcggtg aggatctgac cggtggtgta
    18121 atccatcaat acccaggatc cggcatgaat ctcaggaggc tgaggtgaaa aaggaatgtt
    18181 ttccgccgca aaaccagacg ataagttaaa aacgaacaaa gaagcagcaa taataagacg
    18241 gcgtttcaac agcaaaccct caggagtttc aaatagctgt tctttttacg gaaatactta
    18301 tgaactggct ggaataaagt gcaagaaaat gtgactaccc tcgcattttt atctgacatg
    18361 atctgttgcc actcgctgtc aaattgtcgc gctaaagctg attagcacgg tgatatttga
    18421 tactctggca gacagcagaa ataacggatt taacctaatg atgaatgacg gtaagcaaca
    18481 atctaccttt ttgtttcacg attacgaaac ctttggcacg caccccgcgt tagatcgccc
    18541 tgcacagttc gcagccattc gcaccgatag cgaattcaat gtcatcggcg aacccgaagt
    18601 cttttactgc aagcctgcgg atgactattt accccagcca ggagcagtat taattaccgg
    18661 tattaccccg caggaagcac gggcgaaagg agaaaacgaa gccgcgtttg ccgcccgtat
    18721 tcactcgctt tttaccgtac cgaagacctg tattctgggc tacaacaatg tgcgtttcga
    18781 cgacgaagtc acacgcaaca ttttttatcg taatttctac gatccttacg cctggagctg
    18841 gcagcatgat aactcgcgct gggatttact ggatgttatg cgtgcctgtt atgccctgcg
    18901 cccggaagga ataaactggc ctgaaaatga tgacggtcta ccgagctttc gccttgagca
    18961 tttaaccaaa gcgaatggta ttgaacatag caacgcccac gatgcgatgg ctgatgtgta
    19021 cgccactatt gcgatggcaa agctggtaaa aacgcgtcag ccacgcctat ttgattatct
    19081 ctttacccat cgcaataaac acaaactgat ggcgttgatt gatgttccgc agatgaaacc
    19141 cctggtgcac gtttccggaa tgtttggggc atggcgcggc aataccagct gggtggcacc
    19201 gctggcgtgg catcctgaaa atcgcaatgc cgtaattatg gtggatttgg caggagacat
    19261 ttcgccatta ctggaactgg atagcgacac attacgcgag cgtttatata ccgcaaaaac
    19321 cgatcttggc gataacgccg ccgttccggt taagctggtg catatcaata aatgtccggt
    19381 gctggcccag gcgaatacgc tacgcccgga agatgccgac cgactgggaa ttaatcgtca
    19441 gcattgcctc gataacctaa aaattctgcg tgaaaatccg caagtgcgtg aaaaagtggt
    19501 ggcgatattc gcggaagccg aaccgtttac gccttcagat aacgtggatg cacagcttta
    19561 taacggcttt ttcagtgacg cagatcgtgc agcaatgaaa attgtgctgg aaaccgagcc
    19621 gcgtaattta ccggcactgg atatcacttt tgttgataaa cgaattgaaa aactgttgtt
    19681 caattatcgg gcacgcaact tcccggggac gctggattat gccgagcagc aacgttggct
    19741 ggagcaccgc cgccaggtat tcacacccga gtttttacaa ggctatgcag aagagattca
    19801 gatgctggcg cagcagtatg ccgacgataa agaaaaagtg gcgctgttaa aagcgctttg
    19861 gcagtacgcg gaagagatcg tctagtttga agcctccgcc gctgatacgg cggaggacaa
    19921 tgttgggtca ggctttttga acggtgatac tccacgcagc atcgccaatc tgctgataat
    19981 cggtgatggc atgtccttct tctgccgccc actgtgggat tgcttcagtt gcctgggtgc
    20041 aatcaaactc aattaccaac tcatcgccgc tggccatttc tgccagtgct gcttttgctt
    20101 ctatgagtgg aaacggacag acctgcgtca ccacatccag ctttttaatc gccatcttta
    20161 caccttactt aatttgccgc agcagttgcc agtcgcgcct tacgctgcgg tcgaacatac
    20221 acaagccagg acgcagtcca gactccgaga atcataaata caaggccaac ccagccctgc
    20281 caggtcatca ttgcagtcat aaccagtcca ttaccgatag agcaaccacc tgcaatactg
    20341 gcaccgaagc ccatcagtac accacctagc ccgctacgta atgttgtttg tgcatcagct
    20401 gcgcgaacgc ggaactcacg gctcgctttg gctgcaataa atgaccccac gaagatccct
    20461 aacaccagaa aaacgcccca gttaatgtat ttcacgtcac ccgcgaccag aaattgcaga
    20521 atattggccg ttggggaagt gattcctaac ccaaacatgc gtccggttgc ttcactcagt
    20581 ggccaggcta aaagcgcaat caaaccgatg agtacagcag tgacaaaggg atgccagcgt
    20641 ttttcaaaca gaatatgagc gatcccggtt cggcgcggcg gtaaggtcgc gactttgagt
    20701 tttggcttct tcaactcttt catcaccacc cagagcgtta tcaccagcaa aacggtaacc
    20761 aacggccaca cagacaaatt aaaagtctca gcaatagagt tatgttcagt actgtagtgc
    20821 tgcaaggttt gatttaaacc actggcatgt ggagaacgca tcaccgcact catcaccata
    20881 taagtgaaaa gcgcgatcca actgccgatc aatccctcac ccgcgcgata ccaggtcccg
    20941 gtggcacatc cacccgccag aacaattccc agcccgaaga tatacccacc tataacagta
    21001 ccaagccacg ggaacgcccc ggcttcgtaa gtcagtaggc ccgcctgaat taacgcaaaa
    21061 acccccacgc tttgtaccaa aattgcaatc agcaaggcgt aaaacatgcg attatttttc
    21121 gcgatataca tatcgcgaaa accacctgtc aggcaaaaac gcccacgctg catgacaaat
    21181 cccagcagag caccacaaat tagcccgctt aatatcattg aaaacataaa ttaattaacc
    21241 agatgaatgt taatgaggaa attattcatg actggtggaa tgcagaccaa taaccaaatt
    21301 ctctaaatta gaacaaatgg ttattaatga ggatttcgat aaaaaagaat aaaaaaaccg
    21361 gagccaaatg ttcattcgac tccggttgat tattagaaaa gatgattatg ctacgtcttc
    21421 gtactgcgga accgggttac ggaagctctt ggtgacacaa gccaggtaaa tcagaccgat
    21481 agcagcccag atcagaccca gaaccattga gctttcttcc aggttaaccc acagcgcacc
    21541 aacggtcagc gcaccacaca tcggcaggaa cagatactgg aagtgatctt tcagcgtctt
    21601 attacgcttc tcacggatcc agaactgcga gatgaccgac aggttaacga aggtgaacgc
    21661 caccaacgca ccaaagttga tcagcgccgt agccattacc aggtcgaagt tgattgccag
    21721 cagggcaatc gcgccaacca ggatgatgtt catcgccgga gtacgccatt tcgggtgtac
    21781 ataaccgaag aagcttttcg ggaatacgcc gtcacgaccc attacgtaca tcagacgcgc
    21841 tacgcctgca tgcgccgcca taccggacgc cagtacggta atggtggaga agatcagcgc
    21901 gccaacctgg aacgctttac ccgcaacgta cagcatgatt tcaggctgtg acgcatccgg
    21961 atctttaaag cgagagatat ccgggaagta cagctgcagg aagtaagttg caaagatgaa
    22021 gatcatgccg ccaatcagcg cggtcaggaa aatcgcacgc gggatcacgc gctctgcatc
    22081 tttggtttct tccgacaggt tgctgatgcc gtcaaagccg gtaaaggaga agcacaggat
    22141 tgtcgccccg gtaatcatcg ggataacatg cgcatcgcca gaccagaacg gacgagtgct
    22201 cgccagcgta ccagcgcctt caccttcaaa tacgccataa acaaccatgc ccagaatcac
    22261 cgcgatcagc acgacctgca acacgacgat tacagtgttg aagttcgcta cggatttcag
    22321 actacgcagg ttaaaggcgg tcataaaggc caccagcgcc accacaaaca tccacgatgg
    22381 gatggaaggc accagagctt caaaatagat tttcgccagc agaatgttga tcatcggcgc
    22441 gaacagatag tcgagcagag aagaccaacc caccataaag ccgacagtcg ggctaatgga
    22501 tttctgggcg taagtgtatg cagagccagc agaaggatag cggcgcacca gcttcccgta
    22561 gctcagagcc gtaaacagga tcgcaatcaa tgcgaacgca taggctgtcg gcacatgacc
    22621 atccgtgagg cctgaaacga taccaaaagt atcaaacagc gtcatcggct gcatataggc
    22681 aagacccatc attacaaccg gaactaacgt aagcgtttta cgcaattcca cgcgagaggt
    22741 gtttggagta acgttatgcg acatggtcat tctcctttac ggtgataacc gtcgcgtaag
    22801 caaaaaattg ccccattttt ttggattcct cagcgacaac aactgtcgat ttttagtaaa
    22861 tatctatccg gtacgaagcc cggcctcttg gtatgaatga ttggtttgaa gcaaaaaata
    22921 accgacgctg atgaaacgtc ggtttttagt cattttttga cagcggcgca ttgtgcctaa
    22981 atggggggga aatgacaaga gaatgagagg cttgtcataa taatttttct ttaaatggct
    23041 gattttccgt catcagattt tgcataaaca ccggcgatcg cactatttgc taaaatttca
    23101 tctcgccacc agccgctggc aaggcctgcg gtctgttcgt tccagccaat ccagacgggt
    23161 tcaaagagag tttgggcgac cactttcttc tcgattaacg cgccactatc gagaaaacgt
    23221 tgcgccagat aacggggtaa atagccgcag ccgaggccgc taatttgcag ctccagcttg
    23281 gttttaaaat cgaaaacggt aatggcttct tgttcgtcga gaagttgtga ggccgtagaa
    23341 gcgcctgcct gcgcagtatc ccccaccaca atcgcccgat atcgcttaat aatacgacga
    23401 tttaacggct cttcttccag ggctaacgga tgatgaggcg cgaccgcaaa gacttgttcc
    23461 agatcgccca accgtgaaaa accaaactca ctggaagatg gtggctcatg catcgcgcca
    23521 acaataatat ccgctcgccc ctgagtcagg gcatcccagg aaccaccgag tacgccattg
    23581 ataaacttca ggcgcgtcac gctatgatgt tgataaaaag cttcaatcag tggcgcaagc
    23641 agagaaaaag gaaacgtgtc gtccacgcca atcaccagct cgttttccca gccttcatgc
    23701 agtttgatcg cctgcttttc cagttcccgc acggtatgca gaacttcccg ccctttctct
    23761 aataacattt ttccggtgcg ggtgaattta gcgcggtgtc cgctacgatc aagcaattga
    23821 atattaaggt cgctttccag cttgtgaacg gtgtaactca gagcggacgg cgtcttgtag
    23881 agtttggccg acgccgccgc aaaactgcct tctttttcta aggcatcgag gatcatcaac
    23941 acatccagca gtggtttcat acacgccccc ttgcagtgta tgcggcgtcc tgctggcgtg
    24001 atcgtttact ccagcggcat taccagcgga tcgggatact ggtattcaaa tcccagttca
    24061 ttacaaatcc gactgccatc aataatctta cctttgccgc tgtccagact atttctgaac
    24121 tgcggaggct ccagccccag taaacgggcc atctgcggat agaaaacatt acgcgcaggg
    24181 tgagctggcg cacatatatt atagatgtgt ccgcctttag gtgcctgtaa caacagagtg
    24241 atagcgccaa taacatcttc taaatggact aaattaacac catgttcacc atcaggcgcg
    24301 gtttttccgg caaagaagcg tccgggatga cgccccggtc cgaccaggcc cgcaagacgc
    24361 agaatatcga ccgaagtacc gggtaaattg tgcagccagt cttcgagttc ttctaatact
    24421 cgtccactgt tggttaccgg attacgcggg gtagtttctt tcaccatgcc ttgcgcatcg
    24481 ccatagacag atgttgagct ggtaaaaata attcggggaa tacgatgggc cagcgcgcta
    24541 tccactaact cttgtaccgc ttgtaaatag aactcatcgc cggagccgct acgacgtgcc
    24601 ggaagcgtaa tgaccagtgc atcggcatcc atcagggcat ccagatcgtc agaatcgcaa
    24661 actaactcag gctccatgcg aagcagatag ctatcaatgc cactcattcg ggccgcttcg
    24721 acaccatctt gtgtggtttt actcccggtg acttgccagc ctcgcgctga aagtgacatc
    24781 gccagcggca tgcctaacca ccctaacccg acaattgcga cctttttcat ccgttatctc
    24841 ctgatacacc ttagatctat aaggctacgc tagcgtatca aaactgacaa ttcattctat
    24901 gaatgaatct gttcaataat gataacgaca tgctgcaatg agcagtgaat cgtcggtaac
    24961 cgcgtatacc agacggtgct cctctgtaat gcgtcgggac cagaaacctg acaaattatg
    25021 tttcaggggt tctggcttcc ccttaccttc aaatggcgtt ctgcgggtat ctttgataag
    25081 ttcattgatc tttttaacaa ttcgcttatc tgtttcctgc cagtacagat aatcgtccca
    25141 tgattcctca gaccagatta gtttcactca ataatgtcct tttctgttcc tttgcctgat
    25201 ttcaggctat cgattgagtc catcaatctc cgggcgttag cgggggaacg cagtagataa
    25261 gccgtctctt ccagcgagtt gtattcttcg agtgacatca gaacacaagc ctctccattc
    25321 tgacgagtaa taaggatcgg ggcatgatct tcaacggctt tcatcattgt tgccgacaaa
    25381 ttctgacgcg cttcgctgta gctaattgta cgcatgtcaa tctcctcttt tgtacagttc
    25441 attgtacaat gatgggcgtt aattaactat ttattaatta gtttgtagat caaggtattg
    25501 tcagtgagac gaaaatccag gcttcgctat ttttggtgcc gtcagcttag aggacagtcc
    25561 tcttagcccc ctcctttccc cgctcattca ttaaacaaat ccatcgccat aaaatatata
    25621 aaaaagccct tgctttctaa cgtgaaagtg gtttaggtta aaagacatca gttgaataaa
    25681 cattcacaga gacttttatg acacgcgttc aatttaaaca ccaccatcat caccatcatc
    25741 ctgactagtc tttcaggcga tgtgtgctgg aagacattca gatcttccag tggtgcatga
    25801 acgcatgaga aagcccccgg aagatcacct tccgggggct tttttattgc gcggttgata
    25861 acggttcaga caggtttaaa gaggaataac aaaatgacag acaacactcg tttacgcata
    25921 gctatgcaga aatccggccg tttaagtgat gactcacgcg aattgctggc gcgctgtggc
    25981 attaaaatca atcttcacac ccagcgcctg atcgcgatgg cagaaaacat gccgattgat
    26041 attctgcgcg tgcgtgacga cgacattccc ggtctggtaa tggatggcgt ggttgacctc
    26101 gggattattg gcgaaaacgt gctggaagaa gagctgctta accgccgcgc ccagggtgaa
    26161 gatccgcgct actttacctt gcgtcgtctg gatttcggcg gctgccgcct ttcgctggca
    26221 acgccggttg atgaagcctg ggacggcccg ctctccttaa acggtaaacg tatcgccacc
    26281 tcttatcctc acctgctcaa gcgttatctc gaccagaaag gcatctcttt taaatcctgc
    26341 ttactgaacg gttctgttga agtcgccccg cgtgccggac tggcggatgc gatttgcgat
    26401 ctggtttcca ccggtgccac gctggaagca aacggcctgc gcgaagtcga agttatctac
    26461 cgctcgaaag cctgccttat ccagcgcgat ggtgaaatgg aagaatccaa acagcagctg
    26521 atcgacaaac tgctgacccg tattcagggt gtgatccagg cgcgcgaatc aaaatacatc
    26581 atgatgcacg caccgaccga acgtctggat gaagtcatcg ccctgctgcc aggtgccgaa
    26641 cgcccaacca ttctgccgct ggcgggtgac caacaacgcg tagcgatgca catggtcagt
    26701 agcgaaaccc tgttctggga aacgatggaa aaactgaaag cgctgggtgc cagttcaatt
    26761 ctggtcctgc cgattgagaa gatgatggag tgatcgccat gagctttaac acaatcattg
    26821 actggaataa ctgtactgcg gagcaacaac gccagctgtt aatgcgcccg gcgatctccg
    26881 cttctgaaag cattacccgc actgttaacg atattctcga tagcgtgaaa gcacgcggtg
    26941 atgacgccct gcgggaatac agcgcgaagt ttgataaaac cacggttacc gcactgaagg
    27001 tgtctgctga ggagatcgcc gccgctagcg aacgcctgag cgacgagcta aaacaggcga
    27061 tggcggtggc cgtaaagaat attgaaacct tccacactgc gcaaaaactg ccgccggtag
    27121 atgtagaaac gcagccaggc gtgcgttgcc agcaggtcac gcgcccggtc gcttcagttg
    27181 ggttgtatat tcctggcggc tccgccccgc tcttctcaac ggtattaatg ctggcgactc
    27241 cggcgcgtat agcgggctgt aaaaaagtgg tgctgtgctc accgccgccg attgccgatg
    27301 agatccttta cgcggcgcag ctgtgcggcg tgcaggacgt gtttaacgtc ggcggcgcac
    27361 aggccattgc cgcactggcg tttggtacgg aatctgtgcc gaaagtggac aaaatcttcg
    27421 gaccaggcaa cgcctttgtc accgaggcaa aacgccaggt gagccagcgt ctggacggtg
    27481 cggcaatcga tatgcccgca ggcccgtcgg aagtgctggt cattgccgac agcggcgcaa
    27541 cgccggattt tgtggcttct gatttgcttt cccaggctga acacggcccg gattcacagg
    27601 tgattttact gacgcccgat gctgatatgg cgcgtcgcgt tgccgaagcc gtcgaacgcc
    27661 agttggcaga actgccgcgt gccgaaaccg cacgtcaggc actaaacgcc agccgcctga
    27721 tcgtgactaa agatttagcg cagtgcgtgg agatctccaa ccagtacggc ccggagcacc
    27781 tgatcattca gacccgcaac gctcgcgatc tggtcgatgg cattaccagt gccggttcgg
    27841 tatttcttgg tgactggtca ccggaatcgg caggtgatta cgcctccggc accaaccacg
    27901 ttctgccgac ttacggttac accgccacct gttccagcct cgggctggcg gatttccaga
    27961 agcgtatgac cgtgcaggaa ctgtcgaaag aaggtttctc cgcgctggct tcgaccattg
    28021 aaacattggc cgccgccgag cgcctgaccg cccataaaaa tgccgttact ttgcgtgtta
    28081 acgcccttaa ggagcaagca tgagcaccgt gactattacc gatttagcgc gtgaaaacgt
    28141 ccgcaacctg acgccgtatc agtcggcgcg tcgtctgggc ggtaacggcg acgtctggct
    28201 gaacgccaac gaatatccca cagccgtgga gtttcagctt actcagcaaa cgctcaaccg
    28261 ctacccggaa tgtcagccga aagcggtgat tgaaaattac gcgcaatatg caggcgtaaa
    28321 accggaacag gtgctggtca gtcgtggcgc ggacgaaggt attgaactgc tgattcgcgc
    28381 tttttgcgaa ccgggtaaag acgccatcct ctactgcccg ccaacgtacg gcatgtacag
    28441 cgtcagcgcc gaaaccattg gcgtcgagtg ccgcacagtg ccgacgctgg acaactggca
    28501 actggacttg cagggcattt ccgacaagct ggacggcgta aaagtggtct atgtttgcag
    28561 ccccaacaac ccgacagggc aactgatcaa tccgcaggat tttcgcaccc tgctggagtt
    28621 aacgcgcggt aaggcgattg tggttgccga tgaagcctat atagagtttt gtccacaggc
    28681 atcgctggct ggctggctgg cggaatatcc gcacctggct attttgcgca cactgtcgaa
    28741 agccttcgcg ttggcggggc ttcgttgcgg atttacgctg gcaaacgaag aagtcatcaa
    28801 cctgctgatg aaagtgatcg ccccctaccc gctctcaacg ccggttgccg acattgcggc
    28861 ccaggcgtta aacccgcagg gaattatcgc catgcgcgaa cgggtggcgc aaattattgc
    28921 agaacgcgaa tatctgattg ccgcattaaa agaaatcccc tgtgtggagc aggtttttga
    28981 ctctgaaacc aactacattc tggcgcgctt taaagcctcc agtgcagtgt ttaaatcttt
    29041 gtgggatcag ggcattatct tacgtgatca gaataaacaa ccctctttaa gcggctgcct
    29101 gcgaattacc gtcggaaccc gtgaagaaag ccagcgcgtc attgacgcct tacgtgcgga
    29161 gcaagtttga tgagtcagaa gtatcttttt atcgatcgcg atggaaccct gattagcgaa
    29221 ccgccgagtg attttcaggt ggaccgtttt gataaactcg cctttgaacc gggcgtgatc
    29281 ccggaactgc tgaagctgca aaaagcgggc tacaagctgg tgatgatcac taatcaggat
    29341 ggtctgggaa cacaaagttt cccgcaggca gatttcgatg gcccgcacaa cctgatgatg
    29401 cagatcttca cctcgcaagg cgtgcagttt gatgaagtgc tgatttgccc tcacctgccc
    29461 gccgatgagt gcgactgccg taagccgaaa gtaaaactgg tggagcgtta tctggctgag
    29521 caagcgatgg accgtgccaa cagttatgtg attggcgatc gcgcgaccga tattcaactg
    29581 gcggaaaaca tgggtattaa tggtttacgc tacgaccgcg aaaccctgaa ctggccaatg
    29641 attggcgagc aactcaccag acgtgaccgt tacgctcacg tagtgcgtaa taccaaagag
    29701 acgcagattg acgttcaggt gtggctggat cgcgagggtg gcagcaagat taacaccggc
    29761 gttggcttct ttgatcacat gctggatcag atcgccaccc acggcggctt ccgcatggaa
    29821 atcaacgtca aaggcgacct ctatattgac gatcaccaca ccgtcgaaga taccggcctg
    29881 gcgctgggcg aagcgttaaa aatggctctt ggcgacaaac gcggtatttg ccgctttggt
    29941 tttgtactgc cgatggacga atgccttgcc cgctgtgcac tggatatctc tggtcgcccg
    30001 cacctggaat ataaggccga gtttacctac cagcgcgtgg gcgatctcag caccgaaatg
    30061 atcgagcact tcttccgttc gctctcatac accatgggcg tgaccctgca cctgaaaacc
    30121 aaaggtaaaa acgatcacca ccgtgtagag agcctgttca aagcttttgg tcgcaccctg
    30181 cgccaggcca tccgcgtgga aggcgatacc ctgccctcgt cgaaaggagt gctgtaatga
    30241 acgtggtgat ccttgatact ggctgcgcca atttgaactc ggtgaagtca gccattgcgc
    30301 gtcacggtta tgaacccaaa gtcagccgtg acccggatgt cgtgttgctg gccgataaac
    30361 tgtttttacc cggcgttggc accgcgcaag cagcgatgga tcaggtgcgt gagcgcgaac
    30421 tgtttgatct catcaaagcc tgtacccaac cggtgctggg catctgctta gggatgcaac
    30481 tgcttgggcg gcgcagcgaa gagagcaacg gcgtcgactt gctgggcatc atcgacgaag
    30541 acgtgccgaa aatgaccgac tttggtctgc cactgccaca tatgggctgg aaccgcgttt
    30601 acccgcaggc aggcaaccgc ctgtttcagg ggattgaaga cggcgcgtac ttttactttg
    30661 ttcacagcta cgcaatgcca gtcaatccgt ggaccatcgc ccagtgtaat tacggcgaac
    30721 cgttcaccgc ggcggtacaa aaagataact tctacggcgt gcagttccac ccggagcgtt
    30781 ctggtgccgc tggcgctaag ttgctgaaaa acttcctgga gatgtgatga ttattccggc
    30841 attagattta atcgacggca ccgtggtgcg tctccatcag ggcgattacg gcaaacagcg
    30901 cgattacggt aacgacccgc tgccgcgctt acaggattac gccgcgcagg gtgccgaagt
    30961 gttgcacctg gtggatctga ctggggcaaa agatccggct aaacgtcaaa tcccgctgat
    31021 taaaaccctg gtcgcgggcg ttaacgttcc ggtgcaggtt ggcggcggcg tgcgtagcga
    31081 agaagacgtg gcggcgttac tggaagcggg cgttgcgcgc gtggtggtcg gctccaccgc
    31141 ggtgaaatca ccagaaatgg tgaaaggctg gtttgaacgt ttcggtgccg atgccttagt
    31201 gctggcgctg gatgtccgta ttgacgagca aggcaacaag caggtggcag tcagcggctg
    31261 gcaagagaac tcgggcgttt cactggaaca actggtggaa acctatctgc ccgtcggcct
    31321 gaaacatgtg ctgtgtaccg atatctcgcg cgacggcacg ctggcaggtt ctaacgtctc
    31381 tttatatgaa gaagtgtgcg ccagatatcc gcaggtggca tttcagtcat ccggcggcat
    31441 tggcgacatt gatgatgtgg cggccctgcg tggcactggc gtgcgcggcg taatagttgg
    31501 tcgtgcatta ctggaaggta aattcaccgt gaaggaggcc atcgcatgct ggcaaaacgc
    31561 ataattccgt gtctcgacgt tcgtgatggt caggtggtga aaggcgtaca gtttcgcaac
    31621 catgaaatca ttggcgatat cgtgccgctg gcaaaacgct acgctgaaga aggtgcagat
    31681 gaactggtgt tctacgatat caccgcttcc agcgatggcc gtgtggtaga taaaagctgg
    31741 gtatctcgcg tggcggaagt catcgacatt ccgttttgtg tggcgggtgg gattaagtct
    31801 ctggaagatg ccgcgaaaat tctttccttt ggcgcggata aaatttccat aaactcgcct
    31861 gcgctggcgg agccgacgtt aattactcgc ctggccgatc gctttggcgt gcagtgtatt
    31921 gtggttggta ttgatacctg gtacgacgct gaaaccggta aatatcatgt gaatcaatat
    31981 accggcgatg aaagccgcac ccgcgtcact cagtgggaaa cgctcgactg ggtacaggaa
    32041 gtgcaaaaac gcggtgccgg agaaatcgtc ctcaacatga tgaatcagga cggcgtgcgt
    32101 aacggttacg acctcgagca gctgaaaaaa gtacgtgaag tttgccacgt cccgctgatt
    32161 gcctccggtg gcgcggggac catggaacac ttcctcgaag ccttccgcga tgccgacgtt
    32221 gacggcgcgc tggcagcttc cgtattccac aaacaaataa tcaatattgg tgaattaaaa
    32281 gcgtacctgg caacacaggg cgtggagatc aggatatgtt aacagaacaa caacgtcgcg
    32341 aactggactg ggaaaaaacc gacggactga tgccggtgat tgtgcaacac gcggtatccg
    32401 gcgaagtgtt aatgctgggc tatatgaacc cggaagcctt agacaaaacc atcgaaagcg
    32461 gcaaagtcac cttcttctcg cgcactaaac agcgactgtg gaccaaaggc gaaacgtcgg
    32521 gcaatttcct caacgtagca aatattgcgc cggactgcga caacgacacg ctactggtac
    32581 tggcgaatcc tatcggacca acctgccaca aaggcaccag cagctgcttc ggcgacaccg
    32641 ctcaccaatg gctgttcctg tatcaactgg aacaactgct cgccgaacgc aaatctgccg
    32701 acccggaaac ctcctacacc gccaaactgt atgccagcgg caccaaacgc attgcgcaga
    32761 aagtgggcga agaaggcgtg gaaaccgcac tggcagcgac agtacatgac cgctttgagc
    32821 tgaccaacga ggcgtctgac ttgatgtatc acctgctggt gctgttgcag gatcaggatc
    32881 tggatttaac aacggtgatt gagaacctgc gtaaacgtca tcagtgagtt gcggggtaag
    32941 cggatgcgat gttgtcgtcg catccggcaa aaaaaacggg caaggtgcca ccaccctgcc
    33001 cattttcttt aaaaccgaaa agattacttc gcgttgtaat tacgcagagc attacgcccc
    33061 aacacaatcc ccgcgccaac catgccgccc agcagcaccg ccagaatcaa ggtgattgcc
    33121 tttttcggac tatcgcgacg aataggtaac gtcggtttca ttacataacg gtaagcatga
    33181 atatcaagat catcaacttt taagctttcg atatccagca ggttttgacg agtctgatag
    33241 tagtttggtg agaacaccaa cggacgggtc gcctcatgct taatcatcga ctccagcgct
    33301 tcgctcccca aaaggaacaa cgtatcttgt gtcacatctt cagtctgctg aacctgtggc
    33361 tttgtcacct gcgcctgatt cgcatactgc aacgcttcct gaatctgacg gatacgcaga
    33421 tctttctgct cctgcgcgac tacttcctga gttttcagag aatcctgcaa gtttttcata
    33481 cgcagcgcga tgttgtcttt caggtctttt tccagttctt cattaacctg gtcgtcaact
    33541 tgctggatat acttcgccag ctgtttctga gcaccttcag gtgtttcacc aacataagac
    33601 accgccaatg gtaacgactg gtttttaacg gtaggctcaa tggtcagctt ctctgcttct
    33661 tcctggttat ccagcgtttc cgctaatgct gagaatgcag tgctgtaacg tccgataagt
    33721 gaagcctgaa tgtcggagac tttcggtaca gccggaccgt aaatcacatt catcgcgttg
    33781 gtataaccag cgatttgccc cacatcaggt tgagtaacga tagctgtgga agtccacttt
    33841 tctttggcaa cggccagata ccctatagcc agggcaatag cgacaatgac agagatgata
    33901 attgtcatct tgccgcgcca caactgcacc agtaaatcaa tcaaatcaat ctgttccggg
    33961 tcatggtttt gcccagaaac attattattt tctactctca tcattaccct aacggacgaa
    34021 ataattttaa gtcgccatag tttagcgata atcgccatga atgctatagg ataaatgata
    34081 aaaataatga attattacaa agggaacata agagaatcag ggcgaaaatc gccctgataa
    34141 caggatgtta gtcgctgcca aagagatcgc gggtgtagac tttgtctgcc acatccttaa
    34201 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
    34261 cacgttccaa gcgagaattg aagaatgagt cttctttcat taccggttcg taaatgatta
    34321 cttcaacgcc tttcgccttg atacgtttca taatcccctg aatggaagac gcacggaagt
    34381 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggc ttgcgtgaca
    34441 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgaaatca
    34501 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
    34561 aaccaccata accaaacgac ggattgttgt aatggttgcc aatgcgtggg tcgagacaaa
    34621 cgccttcgat aatctggcga gtattcagcc ctaaactttc tgcatagcta tccagttcat
    34681 taaagtatgc tacgcgcatc gccagataag tattagcgaa aagtttaatc gcttctgctt
    34741 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
    34801 cagcaaaacg ttctgcgcgt tctgaacgct caccaatgac aatacgtgaa ggatggagat
    34861 tatcgtaaag ggctttacct tcacggagaa attccggaga gaaaataagg ttatcgatac
    34921 ccagacgttc tttaattgat ttggtgaaac ctaccggaat tgtagattta atcaccatta
    34981 ccgcgttggg attaatttct gtcacatcgc gaatgaccgc ttccacgctt gaggtattaa
    35041 aataattcgt tttcggatca taatcggtag gtgtggcaat aataacgtaa tcggcatttt
    35101 tatacgcgtc atacttatct gtcgtagcgc ggaaattgag atctttagtc gccagatact
    35161 cttcaatctc cttatcaaca atcggtgact gcttcttgtt aagcatgtcc actttggcct
    35221 gaacgatatc cagtgcaacc acttcgtggt tttgcgcaat cagaatacca tttgaaagac
    35281 caacataacc tgttcctgaa attgttattt tcattagctc tgacttcttc cggttaaaca
    35341 tttagagtgg tcattagtcc accaccacaa cttcatctac tgcgggattt tttaacgctg
    35401 atgcggttga actgtcaaga cagcgatcct gctgtgcgac tctgtattat atcgcttttt
    35461 taatcataaa ttatataaaa aggccataac ctgccgcttt gtataataaa aaagcccgga
    35521 gggtttctcc gggccttgtg ttggttaatt gatttaaatc agattaatcc agccattcag
    35581 tatggaacac accttcttta tcaatgcgct tataagtatg cgcaccgaaa tagtcacgct
    35641 gtgcctggat caggttcgca ggcagaacag cggcgcggta gctgtcataa taggcaaccg
    35701 cagcggcgaa ggtcggaacc gggataccgt tctgtaccgc ataagcgacg acatcgcgca
    35761 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg agccagcagc aggttagcga
    35821 tctgcggatt ttcggcatag gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    35881 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tactcttcag
    35941 acgcagcacg cagctgagag aagccttggg cgtaagaaac gattttgccc agatacagag
    36001 cacgacgaac tttttcgatg aactcagcct tgtcgccagc tggctgcgct tgcgggccag
    36061 agagaacttt agacgcggca acacgctgat ctttcagaga agagatataa cgtgcaaaca
    36121 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    36181 taccggtacc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    36241 catctttttt ggtgaagatg tctttggtga tgtcgatcag gtagctgctc agttcaccgt
    36301 tattccactc ggtaaagatc tgtgccagtt cttcgttggt gaggttcagg ccacctttaa
    36361 gcagagaata ggcttcagca atcagctgca tatcaccgta ttcaataccg ttgtgaacca
    36421 tcttcacata gtgacctgca ccatcggcac caatataggt aacgcatggc tcaccgtcgt
    36481 cagctactgc ggcgattttg gtcaggatcg gtgcaaccag ttcataggct tctttctgcc
    36541 caccaggcat aatggaagga cctttcagcg caccttcttc accgccggaa acaccggtac
    36601 caatgaagtt aaagccttct gcagaaagct cacggttacg acgaatggtg tcctggaaga
    36661 aggtgttacc gccatcaatg atgatgtcac ctttatcgag atatggtttg agggaatcaa
    36721 tagcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    36781 gagattcaac aaactctttc accgtatagt aaggaaccag tttcttgcct gggttttcgg
    36841 caattacttc ttccgttttc tcacgggaac ggttgaaaat agagacggta taaccacggc
    36901 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgacgacg ccgatctgtt
    36961 gctttgacat tgtttactcc tgtcaggggg ggtagccacc gcataattta tacgcgatta
    37021 taaatcgtct catgtgtttc tcagtaataa cacatttggt agtagacgta tattaatgac
    37081 tttaaagccg tgtcatatat attctaattg atcattttcg tagatgtgaa aagttaatca
    37141 tgtatattat aaccatcagg attatttatt tgccaattcc aagcatctcg caacatatcc
    37201 tctaaagaaa gtttcgcctg ccactgtaac tcctttaatg ctaaagaagg gtcagaccaa
    37261 cactcagcaa tatcaccact tcttcgttga gaaaatatgt aattaattgc tattccggat
    37321 attttttgaa atgtattaat gacatcaaga acagaat
//
LOCUS       DAZFRN010000034        36467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFRN010000034 DAZFRN010000000
VERSION     DAZFRN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.034"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            168..1385
                     /gene="alaA"
                     /locus_tag="ACHKUB_001641"
     CDS             168..1385
                     /gene="alaA"
                     /locus_tag="ACHKUB_001641"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO6654673.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1469..2068
                     /gene="yfbR"
                     /locus_tag="ACHKUB_001642"
     CDS             1469..2068
                     /gene="yfbR"
                     /locus_tag="ACHKUB_001642"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO6654674.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2127..3959)
                     /locus_tag="ACHKUB_001643"
     CDS             complement(2127..3959)
                     /locus_tag="ACHKUB_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO6654675.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4046..4696)
                     /gene="hxpA"
                     /locus_tag="ACHKUB_001644"
     CDS             complement(4046..4696)
                     /gene="hxpA"
                     /locus_tag="ACHKUB_001644"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO6654676.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4707..5201)
                     /locus_tag="ACHKUB_001645"
     CDS             complement(4707..5201)
                     /locus_tag="ACHKUB_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO6654677.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5284..5739)
                     /gene="yfbV"
                     /locus_tag="ACHKUB_001646"
     CDS             complement(5284..5739)
                     /gene="yfbV"
                     /locus_tag="ACHKUB_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO6654678.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6077..7279
                     /gene="ackA"
                     /locus_tag="ACHKUB_001647"
     CDS             6077..7279
                     /gene="ackA"
                     /locus_tag="ACHKUB_001647"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO6654679.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7354..9498
                     /gene="pta"
                     /locus_tag="ACHKUB_001648"
     CDS             7354..9498
                     /gene="pta"
                     /locus_tag="ACHKUB_001648"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6654680.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9688..11208
                     /gene="yfcC"
                     /locus_tag="ACHKUB_001649"
     CDS             9688..11208
                     /gene="yfcC"
                     /locus_tag="ACHKUB_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO6654681.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11241..11783)
                     /gene="yfcD"
                     /locus_tag="ACHKUB_001650"
     CDS             complement(11241..11783)
                     /gene="yfcD"
                     /locus_tag="ACHKUB_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO6654682.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11841..12392)
                     /gene="yfcE"
                     /locus_tag="ACHKUB_001651"
     CDS             complement(11841..12392)
                     /gene="yfcE"
                     /locus_tag="ACHKUB_001651"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO6654683.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12448..13092)
                     /gene="yfcF"
                     /locus_tag="ACHKUB_001652"
     CDS             complement(12448..13092)
                     /gene="yfcF"
                     /locus_tag="ACHKUB_001652"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO6654684.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13228..13875
                     /gene="yfcG"
                     /locus_tag="ACHKUB_001653"
     CDS             13228..13875
                     /gene="yfcG"
                     /locus_tag="ACHKUB_001653"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO6654685.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13932..14294
                     /gene="folX"
                     /locus_tag="ACHKUB_001654"
     CDS             13932..14294
                     /gene="folX"
                     /locus_tag="ACHKUB_001654"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO6654686.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14315..15208
                     /locus_tag="ACHKUB_001655"
     CDS             14315..15208
                     /locus_tag="ACHKUB_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO6654687.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIQIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15256..16158)
                     /gene="rpnB"
                     /locus_tag="ACHKUB_001656"
     CDS             complement(15256..16158)
                     /gene="rpnB"
                     /locus_tag="ACHKUB_001656"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO6654688.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16355..17128)
                     /gene="hisP"
                     /locus_tag="ACHKUB_001657"
     CDS             complement(16355..17128)
                     /gene="hisP"
                     /locus_tag="ACHKUB_001657"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO6654689.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17136..17852)
                     /locus_tag="ACHKUB_001658"
     CDS             complement(17136..17852)
                     /locus_tag="ACHKUB_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6654690.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17849..18535)
                     /gene="hisQ"
                     /locus_tag="ACHKUB_001659"
     CDS             complement(17849..18535)
                     /gene="hisQ"
                     /locus_tag="ACHKUB_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO6654691.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNGL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18625..19407)
                     /gene="hisJ"
                     /locus_tag="ACHKUB_001660"
     CDS             complement(18625..19407)
                     /gene="hisJ"
                     /locus_tag="ACHKUB_001660"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO6654692.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19628..20410)
                     /gene="argT"
                     /locus_tag="ACHKUB_001661"
     CDS             complement(19628..20410)
                     /gene="argT"
                     /locus_tag="ACHKUB_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO6654693.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20676..21245)
                     /gene="ubiX"
                     /locus_tag="ACHKUB_001662"
     CDS             complement(20676..21245)
                     /gene="ubiX"
                     /locus_tag="ACHKUB_001662"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO6654694.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21340..22857)
                     /gene="purF"
                     /locus_tag="ACHKUB_001663"
     CDS             complement(21340..22857)
                     /gene="purF"
                     /locus_tag="ACHKUB_001663"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO6654695.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22894..23382)
                     /gene="cvpA"
                     /locus_tag="ACHKUB_001664"
     CDS             complement(22894..23382)
                     /gene="cvpA"
                     /locus_tag="ACHKUB_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO6654696.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23641..24303)
                     /gene="dedD"
                     /locus_tag="ACHKUB_001665"
     CDS             complement(23641..24303)
                     /gene="dedD"
                     /locus_tag="ACHKUB_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO6654697.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24293..25561)
                     /gene="folC"
                     /locus_tag="ACHKUB_001666"
     CDS             complement(24293..25561)
                     /gene="folC"
                     /locus_tag="ACHKUB_001666"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO6654698.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25631..26545)
                     /gene="accD"
                     /locus_tag="ACHKUB_001667"
     CDS             complement(25631..26545)
                     /gene="accD"
                     /locus_tag="ACHKUB_001667"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO6654699.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26701..27360)
                     /locus_tag="ACHKUB_001668"
     CDS             complement(26701..27360)
                     /locus_tag="ACHKUB_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6654700.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27443..28255)
                     /gene="truA"
                     /locus_tag="ACHKUB_001669"
     CDS             complement(27443..28255)
                     /gene="truA"
                     /locus_tag="ACHKUB_001669"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO6654701.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWGKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28255..29268)
                     /locus_tag="ACHKUB_001670"
     CDS             complement(28255..29268)
                     /locus_tag="ACHKUB_001670"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6654702.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29334..30470)
                     /gene="pdxB"
                     /locus_tag="ACHKUB_001671"
     CDS             complement(29334..30470)
                     /gene="pdxB"
                     /locus_tag="ACHKUB_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO6654703.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKKYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30569..31564
                     /gene="flk"
                     /locus_tag="ACHKUB_001672"
     CDS             30569..31564
                     /gene="flk"
                     /locus_tag="ACHKUB_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO6654704.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31561..32739)
                     /locus_tag="ACHKUB_001673"
     CDS             complement(31561..32739)
                     /locus_tag="ACHKUB_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6654705.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33023..34243)
                     /gene="fabB"
                     /locus_tag="ACHKUB_001674"
     CDS             complement(33023..34243)
                     /gene="fabB"
                     /locus_tag="ACHKUB_001674"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO6654706.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34402..36408
                     /gene="mnmC"
                     /locus_tag="ACHKUB_001675"
     CDS             34402..36408
                     /gene="mnmC"
                     /locus_tag="ACHKUB_001675"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO6654707.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
BASE COUNT         8998 a         9933 c         9074 g         8462 t
ORIGIN      
        1 cacctcatta ttttgtcatt attaagttat taacagcaca atcgagcctt cccctctggc
       61 aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg
      121 acttccacgg cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg
      181 aaaaatccag caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag
      241 caaaacgcct ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc
      301 cgttcggttt tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg
      361 ctcaagggta ttgcgattcc aaaggtcttt actccgcgcg taaagccatc atgcagcact
      421 accaggctcg tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat
      481 cggagcttat cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc
      541 ctgcaccaga ttacccacta tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc
      601 attatctttg cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa
      661 ttacgcctcg tacgcgtggg atcgttataa tcaacccaaa taacccaacc ggcgcggtat
      721 attccaaaga gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct
      781 tcgccgatga aatttatgac aaaattcttt acgacgacgc tgagcatcac tcaattgcgc
      841 cgctggcacc tgacctgctg accattacct ttaacggact gtcgaaaacg taccgcgttg
      901 caggcttccg tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca
      961 tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg
     1021 ccattcagac cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc
     1081 gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct
     1141 gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca
     1201 ttcacgacga tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc
     1261 aagggacggc attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg
     1321 tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc
     1381 tgtaatctta atttcactgc cggagattgc atccggcagc gttatcccgc cacaatgacc
     1441 tgatgatgtc atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc
     1501 ccgcctgaaa ctcattaacc gctggccgct catgcgcaac gtgcggacgg aaaatgtgtc
     1561 cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa
     1621 atttggcggt aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc
     1681 agaagtgctc accggcgatc tccctactcc ggtgaaatac ttcaattcgc aaatcgctca
     1741 ggagtacaag gctattgaaa aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga
     1801 gctgcgggat atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc
     1861 gctggtgaaa caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc
     1921 cggaaataat gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg
     1981 cagccaggag atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga
     2041 tgagattagc caggattcac cgctgtaagc agccggagtc tgcgtcgcat caggcaataa
     2101 gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca
     2161 caaaccgcca tcacgataat ggtgaacggt acccccaact tcacaaagtc actgaagctg
     2221 taatttcccg gacctaaaac cagtgtgtta acaggtgaag aaaccggtgt cataaaggcg
     2281 gcggatgctg ccatcgccac gaccatcgcg aatggataag gcgacacccc catcgttttg
     2341 gcagcagcca gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaatagc
     2401 ccaataaccg ccgacaagac aaacaaacaa cccagcatca tatgtggccc gtaaccaccg
     2461 ccaatgtcca tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca
     2521 gcaaacggca tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg
     2581 ctttcagcat ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca
     2641 ggattaggaa tttcatctgt cagcattaac gccaccatta gcaccagaca gaaaatggca
     2701 tggggtgcct ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct
     2761 acgaagtcgc ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg
     2821 atgatatcgc ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg
     2881 cgctttagcc ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat
     2941 ttaccaatca gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc
     3001 tggtcagaaa aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat
     3061 tgccggagat cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat
     3121 tcagaaaccc cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg
     3181 atgacgttag cgccataacg ctcacgtaat ttgagatcat ccagccgttg acctatcatt
     3241 ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata
     3301 agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc
     3361 agcatgaaac gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata
     3421 ggtgttacgc taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact
     3481 accaggttcg gcggcgtcgc caccagcgtc atcatgccgc taatcagccc ggcaaaactt
     3541 aacggcatca tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg
     3601 ggaataaaga tagcgacaac gccggttgaa ctcataaacg cgccaagccc cgcgacggtc
     3661 agcatcaaca aaaccaacat tttgatttca ctgttgcccg ccactttgac cagccatgtt
     3721 cccattacgg tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca
     3781 atcaggacaa cgttaggatc agaaaagccg gaaaatactt cggggaccgt cagcgttccg
     3841 cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc
     3901 gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca
     3961 acttatcctt gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga
     4021 cggggctaaa tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg
     4081 tcacagtaat ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat
     4141 ctgccggagc gttaaccgca atgacatggc aacccgccgc caggccagaa agcacgccag
     4201 cgggagcatc ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcaccta
     4261 acagatacgc atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct
     4321 ctggtgcggg aagcccagct attttatggc gcgctcgcgc taccggcatg gagccagaag
     4381 tcacaatggc ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgcccccg
     4441 gaagcgcggt aataccttcg gtttccgtgg cctcgatgtg ctccagacgc gtaaactcgg
     4501 cggcaatatc agcctcggat ttgcccgcca taaaatggcg caaagaggtg atcgcctgtt
     4561 taccgtgaat gaaagccagc acctcttccg gtgctaaccc atgacgtctg gcccagttgc
     4621 tccacgcccg ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa
     4681 aacctttgca ccgcacacgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg
     4741 ctcaaatggt actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc
     4801 cacattggcg tctgggcgtt aaaaccgtga gttccggcgt caaaatgggt atagcgccct
     4861 tccacattaa ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag
     4921 ccgaggaagg tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa
     4981 acatgcaacg catgatacat ctccataatg tcgatgatag tgcggcaggt ttcttctttc
     5041 agctcgccaa actcgcgatc caattcacgc atctgtaatc cgtaaccacg ctcaataatt
     5101 gtttgcaggc gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac
     5161 tgattagaca aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact
     5221 atactacggt gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt
     5281 caattacaaa tcatcaagga aagttttatc cagttgtttg aaggcgcgct taagcgtgtc
     5341 agctaatgcc tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg
     5401 caatttaccg cgaacttcat aaaaccagtt aaggattgca gggggtaatg gcgttacaga
     5461 acgcttgccc agccaccaca atccctgcat gggtaaactt aaggcgaaca gcgcagtggc
     5521 aactgccggc ccaagctgac cgcccagggc aatctgccag cagagagtaa atacggcgat
     5581 cggcggcata aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac
     5641 cggggcaagg cgtttttcca gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa
     5701 gctaaaaaaa ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata
     5761 taaagattca aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg
     5821 tcttccatac ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc
     5881 attgagtcgt caaattcata tacattatgc cattggctga aaattacgca aaatggcata
     5941 gactcaagat atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa
     6001 atgtcggtgt catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc
     6061 aattataggt acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc
     6121 actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga
     6181 atgtttccac ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc
     6241 ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct
     6301 ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg
     6361 cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga
     6421 tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct
     6481 gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca
     6541 gactatgccg gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg
     6601 catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa
     6661 aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg
     6721 ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggcc tgaccccgct
     6781 ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct
     6841 gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg
     6901 cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac
     6961 gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg
     7021 tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gtattcactg gtggtatcgg
     7081 tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga
     7141 agttgatcat gaacgcaacc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga
     7201 aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc
     7261 gagccgcctg actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc
     7321 cgccaaatcg gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct
     7381 accggaacca gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc
     7441 aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg
     7501 cccgatcaga ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg
     7561 ctgaaaatga gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa
     7621 gagatcgtcg caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt
     7681 ctggtcccga cacgtaagca ccagtttgcc cagtctctga actacgaaat cgctaaaacg
     7741 ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa
     7801 gagcgtatcg aactgacccg caacagcttc ggcggtgcca aaaacaccaa catcaccggc
     7861 gttatcgtta acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccggatctg
     7921 tccgagattt tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg
     7981 caagaatcca gcccgctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg
     8041 actcgtgcga tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc
     8101 aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag
     8161 cacttccgtg ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggtggcc
     8221 gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac
     8281 gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta
     8341 tttatggtga acaccaacac ctggcagacc tctctgagcc tgcagagctt caacctggaa
     8401 gttccggttg acgatcacga gcgtatcgag aaagttcagg aatacgttgc taactacatc
     8461 aacgctgact ggatcgaatc tctgactgcc acttctgagc gcagccgtcg tctgtctccg
     8521 cctgcgttcc gttatcagct gactgaactt gcgcgcaaag cgggcaaacg tatcgtactg
     8581 ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc
     8641 gcaacttgcg tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt
     8701 gtagaactgg gtgcagggat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt
     8761 ggtcgtctgg tcgaactgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag
     8821 ttggaagaca acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg
     8881 gtttccggtg ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa
     8941 actgcaccgg gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt
     9001 tacgtttacg gtgactgtgc gatcaacccg gatccgaccg ctgaacagct ggcagaaatc
     9061 gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc
     9121 tcctactcca ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact
     9181 cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct
     9241 gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct
     9301 accgtgttca tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt
     9361 tctgccgacc tgatctccat cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac
     9421 ctgtcccgtg gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag
     9481 tctgcacagc agcagtaatc tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc
     9541 ggaaataatc actatttccg gttttttatt ctcttaattt gcattaatcc tttctgatta
     9601 tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc actttgcata cttaccactt
     9661 tgttttgtgc aagggaatat ttgcgctatg tccgcaatca ctgaatccaa accaacaaga
     9721 agatgggcaa tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagcctt
     9781 gccacctggg tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat
     9841 ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat ttcgcattct gactaacgaa
     9901 gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc
     9961 ctgatgaact tcccgtttga aggattaacc tcaggatcga aatacgggac agccgttggc
    10021 atcatcatgt ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc
    10081 attgataacg gtatcctggc gcttattcgc catacccgcg ggaatgaaat tctctttatt
    10141 cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat ttggtatggg agaagaggcc
    10201 gtcgcctttg ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc
    10261 accgtcctgg tgacctatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg
    10321 ttttgtgtgg tcgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg
    10381 cgcatcgtgg tgtgggttat cgccactatg attggcctga tctttaccat ggtgtacgcc
    10441 tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt
    10501 gaaaagcagg cggatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt
    10561 gtcctgaccg ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt
    10621 ccagaaattg ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt
    10681 ttccgcctta acggcatgac ggttaatacc atggcttcat cctttaccga aggggcgcga
    10741 atgatgatcg cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggtcggt
    10801 aatggtgaag cgggtgatgc cagcgtgtta aataccatcc tcaacagcat tgccaatgcc
    10861 attagcggtc tggacaacgc ggtcgcggcc tggtttatgt tgctcttcca ggcggtattt
    10921 aatttcttcg tgacgtccgg ttctggtcag gcggcgttaa ccatgccgtt actggcaccg
    10981 cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc tggcattcca gtttggtgat
    11041 ggcttcagtc acatcattta cccaacctca gcttcgttaa tggcaactct cggtgtgtgc
    11101 agggtggact tccgtaactg gctgaaggtg ggcgcgaccc tacttggact gctgtttatt
    11161 atgtccagcg tcgtagtgat cggcgctcag ttgatgggct accactaaaa atgttaagag
    11221 ccgcaattgc ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca
    11281 ttgcgcttca tccacaacgc cagcgctttc agcgagtctg gagtgaactc atcgcagcgt
    11341 gcggtgattt cttccggcgt cagccagcaa acttcactga cttcatcttc ctgtagggcg
    11401 aagggaccgt gagagacgca gctgaacaac gcgccccaga cacggcaatt tttatcttcg
    11461 aaatagaact gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc ttcttccgct
    11521 tcgcgacgcg cggattccag cagttgctca tcggcctgga ccacaccgcc tgcggtcgca
    11581 tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc
    11641 atgccatcat gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgt
    11701 tcccggctgg cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta
    11761 cttgccaaac gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg
    11821 cattttgagt tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca
    11881 ttgagtgcga ttacactaag tacgtcatta tccagcatgc cataactcgc cggattaccg
    11941 cctttcggaa tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgcc
    12001 actggcagat gggtatgacc gtacaccagc acatcgttct ggtttaaagc aggtagattt
    12061 tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt
    12121 tgccacggcg cggttatcgg gaaatgcagc agcatttgat ccacttcgct gtcgcagttg
    12181 ccgcgcacag cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc
    12241 gcgtaaccct ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc
    12301 agccactggg caccgctttg ggcaaacaac tccagaacac gttccgtcgc cggtaacgac
    12361 ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag
    12421 ctatgatact ggattctgtc gttgctatca gccagattgc ttcgccgaaa gtgcaataaa
    12481 acgctggaca gacgctcgct gccactggaa tgtcgcataa tccaccaggc gttctggcac
    12541 ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca
    12601 ccattcacca aataaattcg gctgacccag tgctaacaaa tgttctgcca tcgcgaacag
    12661 tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac
    12721 aacatccgtc ggacgctctt cgcggatggg catcagatcg ctgcgcagcc aggcctgaat
    12781 ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg
    12841 cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc
    12901 gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gctgcaaatg
    12961 ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt cctgcaacgc
    13021 cacccaggcg gataacacat aaggggagaa aaagtgggca tctgaccaaa gcgtgatagc
    13081 gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt
    13141 ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata cttgtggtgt
    13201 tcagatgatt aaacaaccgg agctgcaatg atcgatctct atttcgcccc gacacccaat
    13261 ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg attatcgctt gattaaggta
    13321 gacctgggga aaggcggtca gtttcgtccg gaatttttgc gcatttcgcc caacaacaaa
    13381 attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgttaag cctctttgag
    13441 tctggtgcca ttttgttgta tctggctgag aaaacaggac tctttttgag tcatgaaacg
    13501 cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg
    13561 cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa
    13621 cgttatcagg ttgaaactca gcgtctttac catgtactga acaagcggct ggaaaactcg
    13681 ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat
    13741 gcctggactc gccagcgaat tgacctcgca atgtatccgg cagtcaagaa ctggcatgag
    13801 cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag
    13861 cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg
    13921 gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttgcgta
    13981 cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga
    14041 cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt
    14101 atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg
    14161 aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg
    14221 aagtggagat cgataaacta cacgcgctgc gctacgccga ttcggtatcc atgaccttga
    14281 gctggcagcg ttaaccgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac
    14341 gggattaatt ggtcgccatt tgattccgcg tttgctggag ttgggccatc aaatcacggt
    14401 agtgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga cactatggca
    14461 agggctagcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc tggccggaga
    14521 accgattgct gataaacgct ggactcacga gcaaaaagag cgtctctgcc aaagccgctg
    14581 gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccgc cgtcggtact
    14641 catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga
    14701 ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag agattgcctg
    14761 ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg tactggcacc
    14821 ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg gcgggccgat
    14881 tggttccggt cggcagtatc ttgcctggat tcaaatcgat gatatggtca acggcattct
    14941 ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct acccgatacg
    15001 caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta ttttgcgcgt
    15061 ccccgcaacc gccattcggt tgttaatggg cgaatcttca gtactggtat taggtggaca
    15121 acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgttggt acgatttaga
    15181 agaggcgctg gcggatgtcg ttcgctgatg tggtttactg caaacatccg ccagttaact
    15241 cccggtgtta cagggttagt ggctttgcgc tataagatcg tctggcgata gtcgggtcac
    15301 catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa tacgcagggc
    15361 ttcttcgcgt ttgcccagaa ttaatccatc attccgccct tcttcacgta atctgtcagc
    15421 aatggtcatc agtttctcct tttcttgtgg tgcgcgttct gctatctcac caataaatgc
    15481 acggaaacgc tgggcatctc ccgtttgtaa tacataatta aacagggctt ttagctgtct
    15541 gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc ccaacagatc
    15601 gcgctgacga atatgtttct gaattaactc caacagcgcc attttgcggt gttgcataat
    15661 ctcgtcatcc ggcaccacgg taatatccac caacggaaaa gccgatgaat atattttacg
    15721 ggcaatcgca ggctcagcaa attcatcaag ccagcagagt gaataaggat aaggacttct
    15781 gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc ccgcatcaag
    15841 atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta attcttccgg
    15901 cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg ttttcacaga
    15961 ccacaagagg tcggaataat attgccgcag gtcgtcatca ataaaactgt ttggttccag
    16021 tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa tatcaataaa
    16081 atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga
    16141 agttgtcgat attgtcatgg cgttccgtca cccatcaaat ttaagatgac gcgacaataa
    16201 ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa ttcagaagta
    16261 tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat tccccatgaa
    16321 aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc ttaaggaact
    16381 gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgcct tcttcctcta
    16441 ttttcccctg atggaggaaa atgacgtgag tagaaacatg gcgagcaaag cccatttcgt
    16501 gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataatg cgcagcactt
    16561 cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa acttccggtt
    16621 ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa agatgcaccg
    16681 gatatttccc ctgcgcacgt tcgtctatcc cgactttcgc cagatacttc accgcccgct
    16741 cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg acgttttcca
    16801 gcaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg cgtgtgcgca
    16861 gtaagcgcag ttgattttta tcggcgactt tgagttgacc atctttgtcg cgcaccagat
    16921 tgatcgtctg gccattgacc acgatcgacc cttcactcgg tttttcgagg aagttaatgc
    16981 agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt acatctccgg
    17041 cattcgcttg cagtgagacc cctttcagca cttcatgttc gccgtagcgt ttgtgcaaat
    17101 cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga aggtttcaca
    17161 tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata agagatgatt
    17221 aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata
    17281 tcgcgggcta ttttcagcag atccggcaca gtggccgtaa acgccagcgc agtagagtgc
    17341 agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa
    17401 ataatgcaac gatacatttt aaaagtcgag aagccatagg ctctggcggc ttcaatttcc
    17461 ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta
    17521 agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt aaggaattcg
    17581 gttcccttaa caatctcaag cgtgtacatg ccggaataga acaccagcaa ctgaacatac
    17641 agcggcgtac cacgaaaaat ataggtaaat aaccagattg gaaactggat gtatttatta
    17701 ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac taccgacaaa
    17761 ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt ccacagcagc
    17821 ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca cccacggagt
    17881 agcggcgctc aaggaacagc agcacaccat tggaaacagt ggtgaaaacc aggtaaatca
    17941 cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc agttgggtgg
    18001 ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg aggatcacct
    18061 gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac atgatccgcc
    18121 gaaacacttg cccacgagta aaaccgaacg ccgtcgccgc ctctatatgc cctttcggca
    18181 ctgccataaa cgcgccacgg aacgtttcgg taaaataagc accgtagata aaaccgagag
    18241 taataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc gcctccgtca
    18301 ccgtgtttag tgcaatctgc aaaccgtaga aaatcagcag catcaacacc aagtccggca
    18361 cgcctcgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccca ttttgcgaga
    18421 gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagaa ctgatagcca
    18481 gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac aacatgctgc
    18541 ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac ccgaagggag
    18601 cgggctttct ttcaccgatg gcgattaacc gccataaaca tcaaaatcga agtacttttt
    18661 cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt tcagtgcctc
    18721 gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa acagtttttc
    18781 atctttaaca gacgggccac cgaatttgta atctttaccg acaggttgtt tgaggaaacc
    18841 ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag tcaggtcaga
    18901 ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg cccaatgttc
    18961 attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc ctttcagcga
    19021 ctcgactgtc ggctgaatgt cggaattttt cgccaccacc aaacgagaat cggcagcgta
    19081 cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa gcgatgacat
    19141 gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg gattttcgac
    19201 aaacgtacat tgcgtattga tgcgtttgca taattccttt gccagatcga tatcgaagcc
    19261 aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt cggtaccgat
    19321 gcggatgttt tgcggaatcg cagcaaacgc cgcagttgcg ctggagaagg ccagaaccag
    19381 agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt ttatagggaa
    19441 tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc aaaatatccc
    19501 gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc aatgcaccac
    19561 gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac agcttcccag
    19621 cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc ttgtcgtagg
    19681 tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt tcagcatcat
    19741 ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt actgatgagc
    19801 cagcaaaggc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt
    19861 catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc aaatcctggt
    19921 tggcataggc caccacatcc acgcctttgc tacgccaggt ctcgttagcg taagcttcct
    19981 gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc gttggctgaa
    20041 tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg tcggagaagg
    20101 caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatggcg tcgatttttt
    20161 tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta catttcacct
    20221 gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa tcacctttag
    20281 catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc gtctccggta
    20341 gcgccgcata gctggaagcc gctgcggaga gaccgactaa caaagacaga gcgagaatcg
    20401 acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg
    20461 tgtttgcagg ctctttcatg caggtcttat gccatcttga cgacaacagt aacattcaac
    20521 gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag caggtatagc
    20581 ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg
    20641 ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca gcgggcaaag
    20701 agatcttcag gaagggttat tgcaaactgg tcaagaacac gattaaccgt ctgatttatc
    20761 acatcatcaa gggattgcgg gcgatgataa aacgccggaa cgggaggcat aatcaccgca
    20821 ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg tgtttcacgc
    20881 acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg ggtcagtaag
    20941 ccatcagtat agctatggac aatgccggaa agggttttga ttgaacaggg taaaatcacc
    21001 atccccagcg tctggaaaga accggaagag atgctggcgg aaatatcgcg cgcatcgtgc
    21061 gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc
    21121 tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc gcgcagaacc
    21181 tgtaataagc gcacgccata aatcgcgccg ctggcaccgc tgatgcctac aatgagtcgt
    21241 ttcataaata gttgccctgt cagacttgca ggcagacttt gcagtatttc ggcgggagtt
    21301 gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt tatgcatttc
    21361 gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac gtaacgtatc
    21421 gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt tgaacaccga
    21481 gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca gatcgttcag
    21541 atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa cttcgcgacc
    21601 gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg ggaagcgaat
    21661 ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat
    21721 aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga cgtttttatc
    21781 gcggaactcg gcgcggttgg cattcagttt acggcgcacg gacttacgac gcagctgctg
    21841 gcccggcatg ataaaggtgc ggccaacata gcggttttta acgaagccct ggcggtacgg
    21901 tttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttctg ggatcgggat
    21961 caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca gtttcgtgcc
    22021 catattcaca cgcgcgctgt aaacggaaat tttgtcgatg aacgagtccg ggcgagcaaa
    22081 gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt gacgggtaaa
    22141 caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac gcaggaaatc
    22201 aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg tacggttctc
    22261 gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc ggaaagcaac
    22321 cataccgtgg ccgataatca tcgccacaca ggcatatgcg ccgcggatta agcggtttgt
    22381 ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga agttgtccag
    22441 ctcgctagcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg
    22501 tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc
    22561 aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg cgctggagct
    22621 gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac gctgcatatg
    22681 gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga agcagttatt
    22741 ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc
    22801 atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac cgcacatacg
    22861 tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga aacttgacga
    22921 gctttgcaga taatcaaaaa accatctgat gataaaactg aactgcggga tcagctgtga
    22981 tttgctccag tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga atagaatggc
    23041 ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc gatcggtgcc
    23101 tgacaacccc gttttctcca ccagctggcc tatcacgaag ttcacgatag caccaacgat
    23161 cagggtagcg ataaatagta ccgcgatggc aatcccattt cgaaccagtt cgtcttcaaa
    23221 gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc
    23281 ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa ccagagagga
    23341 aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc cacgatttta
    23401 cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat
    23461 ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt
    23521 atgcctttgc ggcatcgtgc aatgcgtgtc ggatgcggcg taaacgcctt atccgaccta
    23581 cggttctaac cctgcccagt cctgataaga cgcgccagcg tcgcatcagg caaggccgta
    23641 ttaattcggc gtatagccca ttaccacgcc acttaagcca gaaagttgct tcaactcacc
    23701 cagcgaacct ttcagcttat ctttcgaggc atccggccca accagaatac gggtaatttt
    23761 accctgcact ggcgtggatg gcgacgtata aacccgataa ccggcaccgc gcagcttacc
    23821 gacaatctca tttactttat cggcattttt cagcgcaccc agttgcacaa cataggcttt
    23881 acccgttggc gctgcttttt cttccacgac cggcttcggt tctggcttcg gcgcaggtgg
    23941 tgcttcaacc ttcggtttag gcggctccac cggtttcggc ttcggtgggg cgaccggtgc
    24001 aggttccggt tcaaactcgg tgttattggc tgcaatagtg gccggatcga gcgacggtgc
    24061 tgcggcatca cctgcccgca cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc
    24121 ctgggtggcg gctggcatca tatcaggctc atcacgatcg cccgctttcg gcaccagcgg
    24181 gatagccgcg aactcatcct gataatgttt tttctgcccg tccagcagcc ctggaagtac
    24241 aatcaccccc agcgccacca gcacgatcgt gcccactaac cgattctgaa acttacttgc
    24301 caccgcttct cctcgcgtca atcacttcca tgacatgtgc gaccgtgtgg aaagaaccac
    24361 acaccagcac ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa
    24421 cgctatcaaa tgatttgccg ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc
    24481 gcggcccttc cagtggcgca caataccagt catcaaccac gcttttcaac caggccagag
    24541 ttccggcaat atctttatca tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg
    24601 gtagcgcttt catacgcccg gtgagatatt ccgccgcatg tggattatgc gcgacatcaa
    24661 aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa
    24721 tcccgtcgcg aatggcattt tcactgactt ccagcccgct ggcacgcagt gccgccagcg
    24781 ctgttgcggc attcggttgc gggacaagcg gcaacggcag attttccagt gtgccgtgag
    24841 catcgctaaa cgcccagtca tgatcggtga cggaatagtt ccactcaacg ccccgacgtt
    24901 gtaacagtgc acctttttcc tgcgccacat cagcaatggt agaaggcatt tccggctcac
    24961 cgacaattgc cggttttgcg ctgcgaaaga tgcctgcttt ctcgcgacca atactttcgc
    25021 gatctggtcc cagccagtcg gtatgatcca gcgcaatgct ggttactaca gccacatcgg
    25081 catcgacaat attggttgcg tccagacgac cgcccagccc tacttccaga atcactacgt
    25141 caagttgtgc ctgtttgaac agccacaacg ccgacagcgt accgtactcg aaataggtca
    25201 gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg
    25261 gcaattcctg cccctgcaca cgtacgcgct cggtataacg caccagatgc ggtgaactgt
    25321 agacgcccac tttgtaccct gccgccatca gaatcgactc cagcgtacgg caggtggtgc
    25381 ctttgccatt cgtacccgca acggtaaaca caaatggcgc tggtttcagg acgccaagac
    25441 gcgccgcgac ctggctcacg cgctcaaggc cgagatcgat agttttactg tgcaggtttt
    25501 ccagataaga aagccacgaa gccagaggcg acgcggcttg aggagtgcgt ttgataatca
    25561 tggtatccgc tgattcgtta cggtgagaat agcaaaaggg cagagccagt ggccctgccc
    25621 ttatcagtta tcaggcctca ggttcctgat ccggtaccgg gggtaccact acgccttcac
    25681 gcggcgcttc aggattcggc gctggcagat tcatcaactt cgccagaatg ctcgccagtt
    25741 tcaggcgcat ttccggacga cggacgatca tgtcgatggc gcctttctcg atcaggaatt
    25801 cactgcgctg gaatcctggc ggcagttttt cgcgaacggt ctgttcgata acacgcggac
    25861 cggcaaagcc aattaacgct ttcggttcag cgatgttgag atcgcccagc atggcgaaac
    25921 ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct
    25981 cctgcatttt tgccagtgcc gcagaggttt tcgccatctg catcagcgac atcagtgctt
    26041 cctgcatacg tgcgccacca gaggcggaga agcagatcag cgggcagtta tcttccagcg
    26101 cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga ccccattgaa ccgcccataa
    26161 aggcgaactc gaatgccgca gcgacaaccg gcattccata cagagtgcct ttcatcacca
    26221 ccagcgcatc tttttcgcca gtttctttct gcgcagatgc cagacggtct ttatacttct
    26281 tggagtcacg aaacttcagc acatctttcg gctcaagctc gctacccagc tccacaaggc
    26341 ttccttcatc taacaggcta tgcaggcgat tacgcgctgt catacgcatg tgatggtcac
    26401 acttcggaca gacctcaaga ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc
    26461 tatcacactt agtccacacc ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc
    26521 ttttaattcg ttcaatccag ctcattaggg acctttctgt ctgaacctgg ttcgatgcca
    26581 gttttatctt tggggacgca taatgccatt tttgccccca acagaccatg aatgttgcac
    26641 attaaaacat aacagcccga aactttggat aaaaaagtgg tcgaaccgcg gagttacttt
    26701 ttattttgcg gcgcgtgccg cagcgcgttt gtgacgaata aattcgaaaa ccccaggcaa
    26761 tattgaaaca aaaataatgc caacaatcat cagcttaagg ttgctctgaa tgaaggggag
    26821 cgtgccgaag aaataacccg cgtaggtaaa aatcagtacc cacaacagtg cgccgatcac
    26881 gttataagcg gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa acggcgcgaa
    26941 cgttctgacg atcggtacaa aacgggcgag aataatcgtt ttgccgccat gtctctcgta
    27001 aaactgatga gtcttatcaa gataactgcg acgaaagatt ttcgaatccg ggttactgaa
    27061 cagtttgtca ccaaataacc gtccaatggt atagttcact gcatcaccga caatagcggc
    27121 aatcaacatc agcacgacca tcatatggac attgagatcg ttagtttcca gtgacgccaa
    27181 tgcacctgca acaaacagca acgaatcacc aggcaggaat ggcgttacca ccagccccgt
    27241 ttcgcagaac agaatcaaaa acagaatggc ataaacccag acgccgtact ccgcgaccag
    27301 ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat
    27361 atttgcctat gccttgtact cgtcatcttt caggctgtaa ctacgttggc tacagcctga
    27421 aatgttccga gcgttattct cgttagtccg ccagaaatag cgggcccatt ggcggttttg
    27481 gaagatcata ccggtcaggg taatccaccg cgaccagata cagcccttcc gcttttgccg
    27541 ttgctgccgc cagcgttctg tcctttgccg ccagcaactc tgctatccag ctctccggct
    27601 ggttgtgggc accgacttcc atcaggctgc cgacaatatt cctgaccata tgatgtacaa
    27661 aggcattcgc tttgatatct accaccacat aaggaccgtg acgcgtgaca ttaatatgca
    27721 taacgttgcg ccacggggtt cgggactggc actgcaccgc acggaacgag gtgaaatcat
    27781 tctcgcccag caagcattgc gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa
    27841 aatgggttac ccctttactc agtaccgccg ggcgcagccg atgattgtag atgatgtagc
    27901 gataacggcg agccgtggcg ctaaatcggg catgaaaatc atcaggtaca gctttacccc
    27961 aacgcacggc gatgtcacca ggtaaattcg catttacgcc caaggtccac gccgcgtctt
    28021 tgcgctgcgc ggtcgtttcg aaatgcacta cctgcccggt accgtgtaca ccagcgtcag
    28081 tacgcccggc gcagaagacg gtaatgggtt cgttcgccac ctgggagagc gccttttcca
    28141 gcttctcctg cacactgcgg acttcattct gccgttgcca gccgtaatac ttactgccgt
    28201 cgtactcaat gcccagcgca attttataaa ctggcggttg ttgctggtcc gacattagta
    28261 cagatactcc tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg
    28321 aacgttatcg gccaccgacc agaactggac ttgctccggc ataccgtagt cattacgcac
    28381 gcagccaaca gaaagatgcg gcgtacccga agcatcccct acctgagtcg ggaattcgtt
    28441 ctcttcagag agcacaatat cttcgccctg agcaaacgca tcacacgctt cttctgccgc
    28501 cagcggacgc agtgcttcaa agttgaccat ctgggcatga ccgtagaata ccggtgcctg
    28561 gacgacgctt gccgaaatca tcagcccttc gtcctgcagg attttgcgta cttcgtcaac
    28621 gatacgacgt tcttcacgca cgctaccttc gctatccggc aacaacggca gcatattgaa
    28681 cgccagttga cgaccgaaga aatcttcttc gtcaatcgga atgccgttga gcaatttcgc
    28741 actctgcccc gctaacgcat cgaccgcttt tttgccctgg gcggaggctg aaatcaggct
    28801 ggtaacactg atacgcgaca aaccgccctg atcgattaac ggtttcagtg ccgccagcag
    28861 ctggctggtc agactgtctg gtacggcgat gacattccgg ttgcggtaat cggtcagtac
    28921 aaacgggttt acttccggca ccaccagcgg tacgtcgggt tcgagagcaa acagtccgct
    28981 gctgtcgatc accaggcaac ctgagttggt cgcttcttca acccaggcag cggtagcttc
    29041 tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg
    29101 cacggtgatt gtcttaccac caaagcgcag ttgttcgcct gcgctttcgt tacgtgccag
    29161 tgcataaatt tccccaaccg ggaactgacg ttcagccagc gtttcaagca gggcttcgcc
    29221 cacagcgcca gttgcgccca ggacggcaat gttccagcct tcagacatgg tggtttactc
    29281 cagaaatagc aaagctccct gccaatgtta cagcagagag catgaagaag agattaacgt
    29341 gccggatgat gaacggcgtt aaaacccagt ttacacagca atgatgccgc actggcgtca
    29401 tcacaaatta catacagaga tgaccattca cggcgctcaa gatatttttt gcgcagttta
    29461 tcgaactcac ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca
    29521 tacaccaaat gcaccagcct tttcagcgtc ggttgatcga gcgggccatg cagcgtaatg
    29581 cgaccaaact ctggcgcggg cagtaatgta tccagcgcaa cgtgctgttc atgcccaata
    29641 aacttgctat aggcttcaaa cacttgcgta gtaccgcgtg ctttaccttc cagggtatag
    29701 cctgcgatat gcggcgtgcc gatatccact tttgtcagca attccacgtt aagttccggt
    29761 tcgccttccc agacatccag cactacgctt aacttctggc cttcattcag gcaggtcagc
    29821 aacgcagtat tatcgacgac tgcgccacgg caggcgttaa tcagaatcgc tcccggcttc
    29881 aggctacgga tcagtttttc atccgccaga tgcagcgttt tgtacggccc gtctttgaag
    29941 agtggcgtat ggaaagtcag aatatccgca tgctgaacta attcatccag cgagcggaaa
    30001 tcaccctcat ccccacggtc agcgcgaggc ggatcgcaaa gcaatgtttt aatccctaac
    30061 gcttccagtc gcgcctgcaa tcgacgtcca acgttaccaa cgcccacgat ccccaccgta
    30121 cggtcgtgca gtgaaaatcc atcgcgttcg gcaagcatca gtagtgagga gaaaacatat
    30181 tccaccaccg caatcgcatt acagccaggt gccgcggaaa aaccaattcc cgcctgcttt
    30241 aaccatgctt catcgacatg gtcggtcccc gctgtggcag taccaacaaa tttaatgggt
    30301 tttcctgcca gcaaagattc attcactttc gtgaccgaac gcaccatcag cgcatccgcg
    30361 tctgccagtt gagcgacggg gattggacgc ccgggaaccg cggtcacctc acccaaacgg
    30421 ctaaataagt cgcgggcata aggcatattt tcatcaacaa ggattttcac gtttgtgtta
    30481 cctgtatgag acgagagtta accggacaag tgtgccataa tctcgcggcc aggcatactt
    30541 gcgaagattt caggtataag gatacgtaat gatacaacct atttccggcc ctcctcctgg
    30601 gcaaccacca ggtcagggag ataacctgcc gtctggcgcg ggcaatcagc ctttatccag
    30661 tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg acctcactga cgcaacagca
    30721 aagagcagaa ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag attcaccgct
    30781 gctttcgcgt cacttccctg ccgctgagca taatctggcg caacgtctgc tggccgcgca
    30841 aaaaagccat tctgcccgcc agcttttagc gcaattaggg gagtatttac gtctggggaa
    30901 taatcgtcag gcggtcacgg attatatccg tcataacttt ggtcagacgc cgctgaatca
    30961 gctctcaccg gagcaattaa aaaccattct caccctgttg caggaaggga agatggttat
    31021 tccgcaacca cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg agcacaatgc
    31081 gctaaaacag ctggtgacca aacttgcggc ggcaacgggg gaacccagca aacagatctg
    31141 gcaatcgatg ctggaacttt ccggggtgaa agatggcgag ttaattccag cgaaactgtt
    31201 taaccatctg gtgacctggt tgcaggcgcg ccagacgtta agccagcaaa atacgccgac
    31261 gctggaatca ctacagatgg cgctaaaaca acctttagat gccagtgaac tggcggcgtt
    31321 atcggcatat atccagcaaa aatatggcct ttctgcgcaa tcatcgcttt cttctgccca
    31381 ggccgaggat attcttaatc agctttatca acggcgggtt aaagggattg atccgcgtga
    31441 tatgcaaccg ctgcttaatc cttttccacc gatgatggac acgttgcaaa atatggcaac
    31501 gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg atgctggtct ggctggttcg
    31561 ttagccccga cgaaatgaca gtatcgtgac aataattccc agcaccgcag agatcgcccc
    31621 ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc attcccgcca gcggcccgga
    31681 gacgccgagg gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg
    31741 tgaggggacg cgtttaacca cctccacgcc cagcgcagga aagataagcg aacatccggc
    31801 tccggttaac gccgcgcccg ctaatgcgac ccacgcacct ggggcttgcc agagcagcaa
    31861 caagcccacc gtttctacaa gcagagagac aatcgccact ttcacgccgc caaaacggtc
    31921 cggcatccag ccaaacatga cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt
    31981 aaagcccgcc atcgcccatc ctttgctggc aaagtagagc gaaacgaaag tcccgataac
    32041 cgcaaaacca acgccttgta gtgccagccc taaccctggt ttccagataa acccgacaac
    32101 gctccacagc gatggacgtt ctcccgccag ggccggtact ttgcgcactg tgccgttaca
    32161 ggcccacgcc agtaagggta ataccattgt ggtgatcgcc agtgcggcaa aaccgtaatg
    32221 gctatgaatc aacaggccaa gcggagcacc aacagcgagg gcaccgtaaa tcgccattcc
    32281 attccatgac atcactttgc cagagtgttt tggccctacg atgcctagtc cccaggtcag
    32341 agcgcctgtc agtaactggc tttcaccaaa gccaagaatc aaacgcccga cgaccaacag
    32401 agcaaatttg aacggtgcgg agacaggcaa aatcgccgcc agcagcaacg cgccgccagc
    32461 cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt gcaccatatt gatcggccag
    32521 tcgcccggca tagccacgcg tcagcaccgt agccagaaac tgaatcccga cggcaatccc
    32581 gaccatggta ttgccatagc ccagatcatg atgaacaaac agcgggataa ccggcaacgg
    32641 cagccctacg gtcatgtagg tgagaaaaac cgcaaaagcg atgcggaaga gcgaaaaatt
    32701 ggcagaagat cgtgtttcgg tttggcttac agcagtcatg cattactcca gaatgcagcg
    32761 caaggcgagg agtatccccg tctcatctct ctggtttcag ggttacagtg cgttggcagg
    32821 atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg cctggaactg
    32881 gcggcgattg tcaatgatgt gaaaaaggga accatcaggt tcccttttgc gttagtgccg
    32941 gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc gacctactcc
    33001 gactactccg acctactgcg aattaatctt tcagcttgcg cattaccagc gtggcgttgg
    33061 tgccgccgaa gccgaagctg ttagacataa cggtagtcag ttcgcgatcg gtcgtttcgg
    33121 tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga
    33181 taaagccgtg ttccagcatc agcagagagt agatagcttc ctgtacgcca gcagcgccca
    33241 gagagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt
    33301 cacggatagc tgccagttct ttcacgtcgc caaccggagt cgaagtacca tgagagttca
    33361 ggtaatcgat tggggtatca acgccgtgca tcgccatctt catgcagcgt actgcgcctt
    33421 cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag
    33481 catagatgtg agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc
    33541 cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg
    33601 gggtgtcgtt gtatttagta gacagcgcac ccatcgcgtc gaattcgcag gccatttccc
    33661 agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct
    33721 ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa
    33781 cgccatgaat tttaaacggc gtggcgaggc aggcagaaac gccggatgcc atcgctttgg
    33841 tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga
    33901 acacctggaa acgcggggaa ccgccgccag aacctgcaat caggccaacg cgcgggttat
    33961 tctggtaagc ttccggagaa aggcccgcat ctgcgattgc ctgctccata gaaaggaatg
    34021 cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat
    34081 ccagttttac gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga
    34141 aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga
    34201 tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt
    34261 cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga
    34321 tcagccattt aatagagaaa tttgcgcagc cttacacaca tcgctaagat cgagccaccg
    34381 cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt
    34441 aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac
    34501 gggctggaag agacgcgtta tgtttttctg ggaggcaacc aattagaggt acgctttcct
    34561 gagcatccac atcctctgtt tgtggtagca gagagcggct tcggcaccgg attaaacttc
    34621 ctgacgctat ggcaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa
    34681 cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg
    34741 catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg
    34801 cccttgcccg gttgccatcg tttattgctc gatgaaggcc gcgtgacgct ggatttatgg
    34861 tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat
    34921 gcctggtttc tggacggctt tgcgccagcg aaaaacccgg atatgtggac gcaaaatctg
    34981 tttaacgcca tggcaaggtt ggcgcgtccg ggcggcacgc tggcgacatt tacgtctgcc
    35041 ggttttgtcc gccgcggttt gcaggacgcc ggattcacga tgcaaaaacg taagggcttt
    35101 gggcgcaaac gggaaatgct ttgcggggtg atggaacaga cattaccgct cccctgctcc
    35161 gcgccgtggt ttaaccgcac gggcagcagc aaacgggaag cggcgattat cggcggtggt
    35221 attgccagcg cgttgttgtc gctggcgcta ttacggcgcg gctggcaggt aacgctttat
    35281 tgcgcggatg aggcccccgc actgggtgct tccggcaatc gccagggggc gctgtatccg
    35341 ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct
    35401 cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag
    35461 ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc
    35521 gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca
    35581 aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt
    35641 aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag
    35701 aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca
    35761 cacagcgtag tggtactggc gaacgggcat caaatcagcc gattcagcca aacgtcgact
    35821 ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca
    35881 gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa
    35941 catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac
    36001 gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa
    36061 gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat
    36121 ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg
    36181 gcggagcaga aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg
    36241 gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag
    36301 atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg
    36361 ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca
    36421 gtgtttgccg gatgcggcgt gaacgcctta tccgacctac gtgtgac
//
LOCUS       DAZFRN010000035        36209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFRN010000035 DAZFRN010000000
VERSION     DAZFRN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.035"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..929
                     /locus_tag="ACHKUB_001676"
     CDS             <1..929
                     /locus_tag="ACHKUB_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6654708.1"
                     /translation="KRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKM
                     AESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQ
                     VRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYGGP
                     EDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVAN
                     LRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKS
                     CATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(985..1527)
                     /locus_tag="ACHKUB_001677"
     CDS             complement(985..1527)
                     /locus_tag="ACHKUB_001677"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6654709.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1511..1822)
                     /locus_tag="ACHKUB_001678"
     CDS             complement(1511..1822)
                     /locus_tag="ACHKUB_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO6654710.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2007..2897)
                     /gene="cyoE"
                     /locus_tag="ACHKUB_001679"
     CDS             complement(2007..2897)
                     /gene="cyoE"
                     /locus_tag="ACHKUB_001679"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO6654711.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2909..3238)
                     /gene="cyoD"
                     /locus_tag="ACHKUB_001680"
     CDS             complement(2909..3238)
                     /gene="cyoD"
                     /locus_tag="ACHKUB_001680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO6654712.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3238..3852)
                     /gene="cyoC"
                     /locus_tag="ACHKUB_001681"
     CDS             complement(3238..3852)
                     /gene="cyoC"
                     /locus_tag="ACHKUB_001681"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO6654713.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3842..5833)
                     /gene="cyoB"
                     /locus_tag="ACHKUB_001682"
     CDS             complement(3842..5833)
                     /gene="cyoB"
                     /locus_tag="ACHKUB_001682"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO6654714.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5855..6802)
                     /gene="cyoA"
                     /locus_tag="ACHKUB_001683"
     CDS             complement(5855..6802)
                     /gene="cyoA"
                     /locus_tag="ACHKUB_001683"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO6654715.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7262..8737)
                     /gene="ampG"
                     /locus_tag="ACHKUB_001684"
     CDS             complement(7262..8737)
                     /gene="ampG"
                     /locus_tag="ACHKUB_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO6654716.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGASLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8781..9359)
                     /gene="yajG"
                     /locus_tag="ACHKUB_001685"
     CDS             complement(8781..9359)
                     /gene="yajG"
                     /locus_tag="ACHKUB_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6654717.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9664..9981
                     /gene="bolA"
                     /locus_tag="ACHKUB_001686"
     CDS             9664..9981
                     /gene="bolA"
                     /locus_tag="ACHKUB_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO6654718.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            10325..11623
                     /gene="tig"
                     /locus_tag="ACHKUB_001687"
     CDS             10325..11623
                     /gene="tig"
                     /locus_tag="ACHKUB_001687"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO6654719.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            11869..12492
                     /gene="clpP"
                     /locus_tag="ACHKUB_001688"
     CDS             11869..12492
                     /gene="clpP"
                     /locus_tag="ACHKUB_001688"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO6654720.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12618..13892
                     /gene="clpX"
                     /locus_tag="ACHKUB_001689"
     CDS             12618..13892
                     /gene="clpX"
                     /locus_tag="ACHKUB_001689"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO6654721.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            14080..16434
                     /gene="lon"
                     /locus_tag="ACHKUB_001690"
     CDS             14080..16434
                     /gene="lon"
                     /locus_tag="ACHKUB_001690"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO6654722.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            16625..16915
                     /gene="hupB"
                     /locus_tag="ACHKUB_001691"
     CDS             16625..16915
                     /gene="hupB"
                     /locus_tag="ACHKUB_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO6654723.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            17107..18978
                     /gene="ppiD"
                     /locus_tag="ACHKUB_001692"
     CDS             17107..18978
                     /gene="ppiD"
                     /locus_tag="ACHKUB_001692"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6654724.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            19129..19500
                     /gene="ybaV"
                     /locus_tag="ACHKUB_001693"
     CDS             19129..19500
                     /gene="ybaV"
                     /locus_tag="ACHKUB_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO6654725.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            19594..19992
                     /gene="fadM"
                     /locus_tag="ACHKUB_001694"
     CDS             19594..19992
                     /gene="fadM"
                     /locus_tag="ACHKUB_001694"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO6654726.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(20044..20739)
                     /gene="queC"
                     /locus_tag="ACHKUB_001695"
     CDS             complement(20044..20739)
                     /gene="queC"
                     /locus_tag="ACHKUB_001695"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO6654727.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(20804..22504)
                     /gene="ybaE"
                     /locus_tag="ACHKUB_001696"
     CDS             complement(20804..22504)
                     /gene="ybaE"
                     /locus_tag="ACHKUB_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO6654728.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQAVTVNELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22604..23422
                     /gene="cof"
                     /locus_tag="ACHKUB_001697"
     CDS             22604..23422
                     /gene="cof"
                     /locus_tag="ACHKUB_001697"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO6654729.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHCDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23575..24033
                     /gene="decR"
                     /locus_tag="ACHKUB_001698"
     CDS             23575..24033
                     /gene="decR"
                     /locus_tag="ACHKUB_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO6654730.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            24063..25835
                     /gene="mdlA"
                     /locus_tag="ACHKUB_001699"
     CDS             24063..25835
                     /gene="mdlA"
                     /locus_tag="ACHKUB_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6654731.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            25828..27609
                     /gene="mdlB"
                     /locus_tag="ACHKUB_001700"
     CDS             25828..27609
                     /gene="mdlB"
                     /locus_tag="ACHKUB_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6654732.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYLQMYQLQLAGEELAASVREEESLSA"
     gene            27790..28128
                     /gene="glnK"
                     /locus_tag="ACHKUB_001701"
     CDS             27790..28128
                     /gene="glnK"
                     /locus_tag="ACHKUB_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO6654733.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVSIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            28158..29444
                     /gene="amtB"
                     /locus_tag="ACHKUB_001702"
     CDS             28158..29444
                     /gene="amtB"
                     /locus_tag="ACHKUB_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO6654734.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29493..30353)
                     /gene="tesB"
                     /locus_tag="ACHKUB_001703"
     CDS             complement(29493..30353)
                     /gene="tesB"
                     /locus_tag="ACHKUB_001703"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO6654735.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30571..31143
                     /gene="ybaY"
                     /locus_tag="ACHKUB_001704"
     CDS             30571..31143
                     /gene="ybaY"
                     /locus_tag="ACHKUB_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO6654736.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(31174..31485)
                     /locus_tag="ACHKUB_001705"
     CDS             complement(31174..31485)
                     /locus_tag="ACHKUB_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO6654737.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            31648..31744
                     /gene="ffs"
                     /locus_tag="ACHKUB_001706"
     ncRNA           31648..31744
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKUB_001706"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            31864..32217
                     /gene="ybaA"
                     /locus_tag="ACHKUB_001707"
     CDS             31864..32217
                     /gene="ybaA"
                     /locus_tag="ACHKUB_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO6654738.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(32259..33809)
                     /gene="pdeB"
                     /locus_tag="ACHKUB_001708"
     CDS             complement(32259..33809)
                     /gene="pdeB"
                     /locus_tag="ACHKUB_001708"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO6654739.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(33973..34443)
                     /gene="ylaC"
                     /locus_tag="ACHKUB_001709"
     CDS             complement(33973..34443)
                     /gene="ylaC"
                     /locus_tag="ACHKUB_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO6654740.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(34559..35110)
                     /gene="maa"
                     /locus_tag="ACHKUB_001710"
     CDS             complement(34559..35110)
                     /gene="maa"
                     /locus_tag="ACHKUB_001710"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO6654741.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPNNVVVGGNPARIIKKL"
     gene            complement(35282..35500)
                     /gene="hha"
                     /locus_tag="ACHKUB_001711"
     CDS             complement(35282..35500)
                     /gene="hha"
                     /locus_tag="ACHKUB_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO6654742.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(35526..35900)
                     /gene="tomB"
                     /locus_tag="ACHKUB_001712"
     CDS             complement(35526..35900)
                     /gene="tomB"
                     /locus_tag="ACHKUB_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO6654743.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         9249 a         8763 c         9619 g         8578 t
ORIGIN      
        1 ctaaaagaat attattttct gtaatgctga tagtttcacc aagcgtggtg gccagcgaga
       61 aagcgcatga attgtatgac agcatatacg gtggtaaacc tgctcccgat gttataaata
      121 cactgcataa aatggctgaa tcgggagata tcgacgcgca gagcctgtta ggctgggaat
      181 attatcagcc tcgttatgat accaaacctg atgttcagga agcgattaaa tggttcgagt
      241 tagcggctaa acaaggtgac agagaagctc cgttagcgct ggggggtatc tactacgacg
      301 gtgagcaggt gcgggtggat tatgccaaag cgtatgcact atttaatcag gccgcgcagc
      361 acggtgtgaa tttagcatgg tccaggttgg gcattatgta cgccaatggt cagtatgttg
      421 aggtagattg taagaaagcg aaggaatatc ttgataaagg tgtccacatt tatggtggcc
      481 cggaagactt tctggctact tgtcgaaaag acatgattga cagaaaaacg gttgacgata
      541 cgttacccgt gattacggtt acccgttctg ggatgcgaga taatttttta gataaaggtt
      601 tttcctgcat ggatagcctc ttcgccacca ccaataaatt aggcgaagtg gccaatcttc
      661 gcgtgacatt cagcattcgt cgcccatccg gtaaggagat taaccaaacg gttggcttcg
      721 cgcctttcgg tttaaaccgg ctgaatatta gctttactga ttacctattt ggttcattta
      781 ccagtaattc gtctcttatt ctttataaac cggaatttga gcgaaaatct tgtgcaactg
      841 taaggaccac aatcgttgcc gcaacggcaa ccattaacgg caaggatgtg gagttgctga
      901 aagcgggggc aattgaacaa aagtggtaac gctgctcagc ccccggctga ggcaaaactt
      961 gccctggcgg gtggcgataa taatctaagc cgcgagaata tgctgaatcg ccaaaaacat
     1021 aggtacagca ccaaaagcat ttggtgtcgc tgaaagctga acaaagccga gacgagcata
     1081 aaaattaatg gcggcagggt cagcatcaag ataaacccct ttaattggta atgcctggtg
     1141 aattattttt acatgttcaa aaaaatcaca tagtaggtcc tgaccaaagc cccgcttttg
     1201 atacttccgt gctaccccca acatgactaa tctgacaaca ccaatttctg aaggttgtga
     1261 accctgaagg acgccagaaa cgcgttgttt ttccagcgaa tatgccgtaa aagtacagat
     1321 gccgatcagt tcaccacttt gtctgtcaat aagtgcttta gccgcacagt cgctattacg
     1381 cacacttttc tttagcgatg cgcgtacaaa tttatcgata acagggttac cgcaatcaaa
     1441 ctgtttttgc ccaggataag taatatcaga ctgataatta caggagagga ctatgggcag
     1501 agtaatctct tcatgcttgt cgaccatcgc tgtttttcct tttcattaac gccttgaggg
     1561 cgagcgtcgg ttgagcaggt tcagcgatga gttggttcat cagttcccag ctttgattcg
     1621 aaagctcacg gcgacgttgg ttatcaagca cactttcggc gcgttccata gcagcgctca
     1681 aaataaaagc gcttaaatct accccaacgg cttgcgcagc ttcacgaagt ttgtttttgg
     1741 cgtcgggact ggttttgagt tcgacccgcg catttttcag cgttttatcg acatcgggta
     1801 gagtggctgt ttttccaggc attatgacct ctttgattaa ctgaatgaaa tcgtagggag
     1861 tcgatctcat acataatacg gctatcaacc gtatgattaa tttatgttca tctgctcact
     1921 ctgtcaagac atacggttat tagccgtatc tctgatctca gacaataaaa aaggtgccgt
     1981 agcacctttt taatagagag gttttgttac cacacagcag ccagcagcgt atgcgagtcc
     2041 ggtaccataa aatcaacgga catcatcacc gagagggcag tgatggcgat gatagagaag
     2101 ccgaacagct tgcgcgccca gattctgtca tcagcaactt tataaccgcg cagagccata
     2161 cctaaccacc agacgctaac cgccgcggcg accaccagat atttatatcc agcgtaaccg
     2221 ccaagagaga gcatcagcgt ggcaacggca aaggcgatga tatacagcgt gatgtgattc
     2281 ttcgctaccg aaatgccttt taccactggc aataccggaa tgtttgccgc ctggtaatcc
     2341 ttaaagcgga aaatggcgat ggcataggag tgaggcatct gccacaggct gaagatagcc
     2401 agcaggatcg ctgcgccgct atcgaactcg ccggttactg cacagtagcc aatcaccggc
     2461 ggcgcagcgc cggagagcga accaatcaat gtgccgtaga ctgagtggcg tttcatgtac
     2521 aggctataga cgccgacata aaccacaaag cccatcaccc ccagccagca ggccagcgga
     2581 ttcgcgccaa accacagcag cataaagcca gcaataccca gcaaggtggc gtacaccagc
     2641 gagacagcag gagagatcag gcctttaacc agcacccgat tcttcgtcct ttccatcttt
     2701 ctgtcgatat ccctgtcgat gtagttgtta aacacacaac ccgacgccac aaccagtgac
     2761 accccaacca gcgtgtagat aaacagggga taatcaatgc tgccctttga ggccagcagg
     2821 aatcccccaa tcaccgagat caggttgcca aagatgatgc ctggtttcgt tacttgcagg
     2881 tattgcttaa acatcataac cgccgctctt agtgcatcat catgttgtag ttgaggttcc
     2941 acataatcca gatggagcct acaaccagga tagcgatgat tagcacggtg aagacaaacg
     3001 ccgtcatgtt ccagccttca tctgatttgg tattcatgtg caggaagcac accagatgca
     3061 ccagaacctg taccactgcc attgccagga ttgttcccag aattacggcc ggagaggcag
     3121 cccctgtcat caccatccag aacggaatca ccgtcagaat gatcgacagg ataaagcctg
     3181 tcatgtaggt ttttacgctg ccatgggacg cgccgctgtg atcggtagaa tgactcatta
     3241 catcgccccc atcagataaa caacagtgaa cacacagatc caaaccacat ccaggaagtg
     3301 ccagaacagg ctcaggcaca tgatgcgggt acggttagtg ctggtcaggc cgcgacgagc
     3361 gatttgtacc atcagcaccg ccatccagat aagaccggaa gtgacgtgca gaccgtgcgt
     3421 gccgaccagc gcaaagaacg ctgacaggaa gccgctgcga tccggaccca tgccgttaac
     3481 aatcaggtga tggaattcgt agatttccat cccgataaat ccggcaccaa acaaccaggt
     3541 caacgccagc caggagataa cctggctttt gttgtttttg tacatggcga tagccgccat
     3601 gccgtaggtg atggagctga acaacagcaa gaaagtttca accagaacga acggcagttc
     3661 gaaaatgtcc ttacctgtcg ggccgcctgc ggtgccgttc accagaacgg cataggtagc
     3721 aaacaagatt gagaacagaa tgcagtcgct catcaggtag atccaaaatc cgaagatttt
     3781 ggttccgcct gcatcgtggt gcccgtgttc gtgcgcgtgg gcagtcgcgt gcgtcaaagt
     3841 atcagttgcc atttttcagc cctgccttag taatctcatc gaaatgctgg ttttccagtt
     3901 tttcgatttc tgccaccggc acgtagtaat ccacgtcctc gtcgaagctt ttcacgatcc
     3961 aggtgatgat catgcctgcg aagccaacaa tcgccagcca ccagatatgc cagatcatgg
     4021 cgaaaccgaa gatggtggag aaagctgcaa tgacgatacc tgcaccgctg tttttcggca
     4081 tatgaatttc ttcatagtgg tcaggctttt tatacgcttc gcctttctct ttcatttccc
     4141 agaatgcatc acgctcgtga acgtgcggca caacggcaaa gttatagaac ggaggcgggg
     4201 aagaggttgc ccactccagc gtacggccac cccacgggtc gccagtcagg tcacggttct
     4261 ggtcgcggtc gcgaatagaa acgtacatct gaataacgag gcagagaata cccagcgcaa
     4321 tcagtactgc accgctagct gcaatcatca gcatggtgtg gaactgcgga tcaatctgct
     4381 ggctcaaacg acgggtcatg cccatgaagc ccagcgcata cagcggcata aaggcaacga
     4441 agaagccgat gatccagaac cagaacgcgc gtttacccca ggtttcattc agtttgaaac
     4501 cgaaggcttt aggccaccag taggtcatcc ctgcgaagca gccgaagacc acgccgccga
     4561 tgataacgtt atggaagtgc gcaatcagga acaggctgtt atgcagaacg aagtccgcgc
     4621 ccggtactgc cagcagcacg ccagtcatcc cgcccaccga gaaggtgacg ataaaaccga
     4681 tagtccacag catcgcagaa tggaacacga tgcggccctg atacatggtg aacagccagt
     4741 tgaagatctt caccccggtc gggatggcga taatcattgt ggtgatacca aagaaggcgt
     4801 ttacgttcgc gcccgcaccc atcgtaaaga agtggtgcag ccagacgatg aacgacagca
     4861 cggtgataca gacggttgcc cataccagcg aggtataacc aaacagacgt ttacgcgaga
     4921 aggttgccgc aatttcggag aacacaccga aaacaggcag gatcaggatg taaacttccg
     4981 ggtggcccca ggcccaaatc aggttgatgt acatcatcat gttgccaccc atatcgttgg
     5041 taaagaaatg ggtgcccaga tagcgatcca gggtcaacaa cgcgacggta accgtcagaa
     5101 ttgggaagga agcaataatc agtacgttcg cgcacagtga tgcccaggta aatactggca
     5161 tcttgaacat ggtcatgccc ggtgcgcgca tcttcagaat ggtaacgaag aagttgatac
     5221 cggtaagcgt cgtacctata ccggatagct ggagactcca tatccagtaa tcaacaccga
     5281 ctcccggact gtactctatt cccgatagcg gtggataggc cagccagccg gtctgcgcaa
     5341 attcgcccac gccgagagaa acgttaacca gaatcacacc aacaacagta aaccagaagc
     5401 ttaagttgtt gaggaacggg aacgcaacgt cacgcgcgcc gatctgcagc ggaaccacca
     5461 ggttcatcag accgataacg aaaggcatcg ccacgaagaa gatcataatc acgccgtgcg
     5521 cggtgaagat ctgatcgtag tggtgaggtg gcaggaagcc cgcttcaccc gccgaggcaa
     5581 gagcctgctg gctacgcatc ataatggcgt cagcaaaacc acgcagcaac atcacaatcg
     5641 ccacgatgat atacatgata ccgaggcgtt tatggtcgac ggaggtcagc cactctttcc
     5701 acaggtaggt ccacttaccg aagtaagtga tcaggccaac gagcgccaga cctcccaaaa
     5761 taatgccagc gatcgtaacc atgacgatag gttcatggaa cgggactgca tcaagtgata
     5821 attttccgaa catctttatt cttcctcaac ccctttaatg ggcggattcc gcgtggctca
     5881 tgtccatgcc ttccatacct tcgtgtgcac tgtgctcacc ttctggctgg gtcatgtcca
     5941 tgctcttacc gtgagccata aacttgttaa tcacatcggc aaacaagtct ggtttcacgt
     6001 tggagaaata ttccacctgg ttgtattcgc taggcgcggc cagtttttcg aacgcagcca
     6061 tgtcagacat ggtgttcggc gactgtttcg cttttgcgac ccattggtcg aattcggcgc
     6121 gatccggtgt tgcaatagct ttgaacttca tgcctgagaa gcccggcccg ctgtagctgg
     6181 cggagatacc gtcataagta ccgggttcgt tggcgatcag atgcaggcga gtctgcatac
     6241 cggccatggc ataaatctgg ctacccagac gcggaatgaa gaaggagttc atcacggagt
     6301 tggaggtcac tttgaagtac accggagtgt tcgccgggaa agcgatttca ttcacggtag
     6361 caatgccctg ttccgggtag atgaagaacc atttccagtc catggaaacc acttcgatgg
     6421 taatgggctt ctcgtcgtgt gccagcggct tgctaggctc aagagcgtga gtggttttcc
     6481 aggtcagtac cgcaaggaag atgatgatta agataggtac cgtccagacc acagcttcca
     6541 ctttattgga gtgtgaccag ttcgggctgt acttagcatc tttattgctc gcacgatact
     6601 tccaggcgaa accaacagcc atcaagattg cgggaataac gacaatcaac atcaggccaa
     6661 atgccgtcag tatcagtgaa cgttgctcca gaccaatctg tcctttggga tctaacagcg
     6721 cagaattaca gccactgagc aatacagtgc ctgcaaataa tgacaaccat cccaaacttt
     6781 tattgtattt cctgagtctc atttaacgac ctcaattcca cgggatctgg tggcgtttaa
     6841 agtgtgtggg cattttacgg gaaggttaca ttactgtaaa catgattaaa ttcctgttac
     6901 ttggatttgg ctgtcaggtc acatatgttg caaaacatat caggttgatt ttaagaagct
     6961 gttgcaaaag ggcgttataa caaaagggga gtgcagaagg agtctgggcg ggcaattggt
     7021 ataaccaatg cgaaataaaa caattattta acaaataatt atcattgcag tgacaactgg
     7081 gtggtgagaa aataaagact cattagctga atcgtgtaag aaaaatagaa tttataaatg
     7141 gcgccaataa tttccaaacg ctaagccgca caaaagaaca aatattaata agcgatgctg
     7201 atgtttattt agccgtaata attacggcga gtgattacta cagctaaata atatttacag
     7261 attacgtcag atgcgttttt cgtagcgcca gataatccag caaaccacca agcacgacgc
     7321 cagaaagggc gactaaaacc ccgacttcca gcagagcagg caggaaagag aagtgcgtca
     7381 aatccagcgc gtccatcgtc aacagcagta accacacggc caacaggctg gcgcccgccg
     7441 ccagtgtcca catggcaaag gcataacctg ccggatatgc ggtacgggag ataaagttgt
     7501 catttactcg tgtatattca agcgtctggc ggcaaaccag cagcaaaata agccccggta
     7561 cggcagcggc gacggagaat agatagaatg tcgaccagcc gtgtgcttca acaaaccaac
     7621 ccgccacggg gccgacataa actcgcccta cagcggaaag cgctgagagc agggcaaatt
     7681 gagttgcgga aaatgactta ttacacagcg tcattaacag cgcgacaaag gctgatgtgc
     7741 ccatcccgcc acagaggttt tcgaaaaaga cggctgcgcc catgctgtag agatgcttat
     7801 cagtaatcga cagcagccag taaccagcgt tagacgcacc ttgtaaaatg ccgaaaatca
     7861 gcagcgcccg gaacagcgac aggcgctgca tcaaaatccc accgtacaat gcgcccacaa
     7921 tggtcgccag taagccaagc gttttgttaa ccacgcctac ttcacccgca tcaaacccga
     7981 cgccgcgaat caaaaacgtg gttgtcaggc tcatggcgaa tgcgtcgccc agcttataca
     8041 gcacgataag aagcaaaata agccaggcat tattgcgacc aaagaaatct cgcagaggtg
     8101 caacaaccgc ttgttccagc gtttttggca caggaatggt gtcggtcggt tctggtgcaa
     8161 gcaacgtcgc gataatacaa gggatcaaca gcgccgccat caaccagtac atgccctgcc
     8221 agcccagcca tttatctgcc agccacaggg ctaagccgcc ggaaaccagc atccctaaac
     8281 ggtaacccag cacgctgatt gccgcgcccg caccacgttc ttctgccggg agcacatcgg
     8341 ttttccacgc atcgaagaca atatcctggg aggccgagca aaaagcgatc accactgcca
     8401 gtgccgccat ccagcggagt tgggtgccag gttcgagaaa acccatcgcc gcaatggcga
     8461 ctaataacag gatttgcgtg gcgagcagcc aaccgcgccg ccgcccaaaa aatgggggcg
     8521 tgtagcggtc catcagcggt gaccagagga atttaaaaac gtaagcctgg cctaccagag
     8581 agaagaaacc aatggttttg agatcgatat tctcgaccgt catccaggcc tgtaaggtgc
     8641 cggatgtcag ggcgagcggt agcccggaag caaaacccag gatcagcaat atggctgaac
     8701 gcggctgttg aaaaatacgt aaatattgac tggacatggg cgtctacagg cccggcttgc
     8761 accgggccag agggcagaaa ttaacgcgcg ttctgcttga tgaattcgtg gatgctggtg
     8821 tcctgagaca tatcagcgat ggtatcggtc agcacactgt taaccgcatc ggcgatattt
     8881 ttgttggagg cctggaacgc accttcaacg ttgtagctgg cacgatagtt tttggtcatt
     8941 ttgttgccat tctgcgcggt agcgatgatg gcgatatccg ctttggtagc gatgttatag
     9001 cgcacgttgc cctgggacac gtcagcatac agttggctaa cgatgatttg cagattaacc
     9061 gggccattcg gaccaaccat gtaaccacgc gcggtcatct gtttttccag cacttcttgc
     9121 agcaggaaac gcagatcgcg ggaggcggtc agggtaacga tttgattatc gcgggtgact
     9181 tttgccagcg cctgatcggt acgctgatcg gcaccattaa tgcttacggt gacgcccatc
     9241 aggcttggat cctgctgtgg cagtgtaatc gtcggggaaa cttcaatagt tgttggcggt
     9301 tttgcgcatc ctgcaagcat aaacagagca actaacggga agaggatttt tttgaacatg
     9361 ttcgggctct cagagactct taagcgtgtt tggtaaaaat tcccgccatc ataacattgc
     9421 caacggcgag gggaagtggg taaggcatgt aaattcatca tgttgacgaa ataatcaccc
     9481 ctggtaaaag aaacgctgat gcgaggcctg tgtttcaatc tttaaatcag taaacttcat
     9541 acgcttgacg gaaaaaccag gacgaaacct aaatatttgt tgttaagctg caatggaaac
     9601 ggtaaaagcg gctagtattt aaagggatgg atgacatctc agcgttgtcg gaggagatat
     9661 ttcatgatga tacgtgagcg gatagaagaa aaattaaggg cggcgttcca acccgtattc
     9721 ctcgaagtag tggatgaaag ctatcgtcac aatgtcccag ccggctctga aagccatttt
     9781 aaagttgtgc tggtcagcga tcgttttacg ggtgaacgtt ttctgaatcg tcatcgaatg
     9841 atttacagta ctttagcgga ggaactctct actaccgttc atgcgctggc tctgcatact
     9901 tacactatta aggagtggga agggttgcag gacaccgtct ttgcctctcc tccctgtcgt
     9961 ggagcaggaa gcatcgcgta aaaacgcatt tgcaactgtc ggcgcttttc cagtatgttg
    10021 ctaaagattt tatgaaaaac ggcctgcggg ccgttttgtt ttgtctggat tttgcgcttt
    10081 ttgcccagca ttcagacgaa aattgcccgg gaattgtgaa aaaatacgcg acagcgcgca
    10141 ataaccgttc tcgactcata aaagtgatgc cgctataatg ccgcgtccta tttgaatgct
    10201 ttcgggatga ttctggtaac agggaatgtg attgattata agaacatccc ggttccgcga
    10261 agccaacaac ctgtgcttgc ggggtaagag ttgaccgagc actgtgattt tttgaggtaa
    10321 caagatgcaa gtttcagttg aaaccactca aggccttggc cgccgtgtaa cgattactat
    10381 cgctgctgac agcatcgaga ccgctgttaa aagcgagctg gtcaacgttg cgaaaaaagt
    10441 acgtattgac ggcttccgca aaggcaaagt gccaatgaat atcgttgctc agcgttatgg
    10501 cgcgtctgta cgccaggacg ttctgggtga cctgatgagc cgtaacttca ttgacgccat
    10561 cattaaagaa aaaatcaatc cggctggcgc accgacttat gttccgggcg aatacaagct
    10621 gggtgaagac ttcacttact ctgtagagtt tgaagtttat ccggaagttg aactgcaggg
    10681 tctggaagcg atcgaagttg aaaaaccgat cgttgaagtg accgacgctg acgttgacgg
    10741 catgctggat actctgcgta aacagcaggc gacctggaaa gaaaaagacg gcgctgttga
    10801 agcagaagac cgcgtaacca tcgacttcac cggttctgta gacggcgaag agttcgaagg
    10861 cggtaaagcg tctgatttcg tactggcgat gggccagggt cgtatgatcc cgggctttga
    10921 agacggtatc aaaggccaca aagctggcga agagttcacc atcgacgtga ccttcccgga
    10981 agaataccac gcagaaaacc tgaaaggtaa agcagcgaaa ttcgctatca acctgaagaa
    11041 agttgaagag cgtgaactgc cggaactgac tgcagaattc atcaaacgtt tcggcgttga
    11101 agatggttcc gtagaaggtc tgcgcgctga agtgcgtaaa aacatggagc gcgagctgaa
    11161 gagcgccatc cgtaaccgcg ttaagtctca ggcgatcgaa ggtctggtaa aagctaacga
    11221 catcgacgta ccggctgcgc tgatcgacag cgaaatcgac gttctgcgtc gccaggctgc
    11281 acagcgtttc ggtggcaacg aaaaacaagc tctggaactg ccgcgcgaac tgttcgaaga
    11341 acaggctaaa cgccgcgtag ttgttggcct gctgctgggc gaagttatcc gcaccaacga
    11401 gctgaaagct gacgaagagc gcgtgaaagg cctgatcgaa gagatggctt ctgcgtacga
    11461 agatccgaaa gaagttatcg agttctacag caaaaacaaa gaactgatgg acaacatgcg
    11521 caatgttgct ctggaagaac aggctgttga agctgtactg gcgaaagcga aagtgactga
    11581 aaaagaaacc actttcaacg agctgatgaa ccagcaggcg taatttacgc agcataacgc
    11641 gctaaattcg cacaaaggcc cgtcaccgcc aggtggtggg cttttttttg tcatgaattt
    11701 tgcatggaac cgtgcgaaaa gcctctttcg gtgttagcgt aacaacaaaa gattgttatg
    11761 cttgaaatat ggtgatgccg tacccataac acagggacta gctgataatc cgtccataag
    11821 gttacaatcg gtacagcagg ttttttcaat tttatccagg agacggaaat gtcatacagc
    11881 ggcgaacgag ataactttgc accccatatg gcgctggtgc cgatggtcat tgaacagacc
    11941 tcacgcggtg agcgctcttt tgatatctat tctcgtctac ttaaggaacg cgtcattttt
    12001 ctgactggcc aggttgaaga ccacatggct aacctgattg tggcgcagat gctgttcctg
    12061 gaagcggaaa acccagaaaa agatatctat ctgtacatta actccccagg cggggtgatc
    12121 actgccggga tgtctatcta tgacaccatg cagtttatca agcctgatgt cagcaccatc
    12181 tgtatgggcc aggcggcctc gatgggcgct ttcttgttga ccgcaggggc aaaaggtaaa
    12241 cgtttttgcc tgccgaattc gcgcgtgatg attcaccaac cgttgggcgg ctaccagggc
    12301 caggcgaccg atatcgaaat tcatgcccgt gaaattctga aagttaaagg gcgcatgaat
    12361 gaacttatgg cgcttcatac gggtcaatca ttagaacaga ttgaacgtga taccgagcgc
    12421 gatcgcttcc tttccgcccc tgaagcggtg gaatacggtc tggtcgattc gattctgacc
    12481 catcgtaatt gatgccagag gcgcaactgt gccgctatac ttatccaggg cggcacaacg
    12541 ctgtaagcgg cttgcgcctg agaatggcat ttgcgtcgtc gtgtgcggca caaagaacaa
    12601 agaagaggtt ttgacccatg acagataaac gcaaagatgg ctcaggcaaa ttgctgtatt
    12661 gctctttttg cggcaaaagc cagcatgaag tgcgcaagct gattgccggt ccatccgtgt
    12721 atatctgcga cgaatgtgtt gatttatgta acgacatcat tcgcgaagag attaaagaag
    12781 ttgcaccgca tcgtgaacgc agtgcgctac cgacgccgca tgaaattcgc aaccacctgg
    12841 acgattacgt tatcggccag gaacaggcga aaaaagtgct ggcggtcgcg gtatacaacc
    12901 attacaaacg tctgcgcaac ggcgatacca gcaatggtgt cgagttgggc aaaagtaaca
    12961 ttctgctgat cggtccgacc ggttccggta aaacgctgct ggctgaaacg ctggcgcgcc
    13021 tgctggacgt cccgttcacc atggccgacg caaccacgct gaccgaagcc ggttatgtgg
    13081 gcgaagacgt tgaaaacatc attcagaagc tgttgcagaa gtgcgattac gacgtacaga
    13141 aagcgcagcg cgggattgtc tacatcgatg agatcgacaa gatttctcgt aagtcagaca
    13201 acccgtccat tacccgagac gtttccggtg aaggcgtaca gcaggcactg ttgaaactga
    13261 tcgaaggtac ggtagctgct gttccaccgc aaggtgggcg taaacatccg cagcaggaat
    13321 tcttgcaggt tgatacctct aagatcctgt ttatttgtgg cggtgcgttt gccggtctgg
    13381 ataaagtgat ttcccaccgt gtagaaaccg gctccggcat tggttttggc gcgacggtaa
    13441 aagcgaagtc cgacaaagca agcgaaggcg agctgctggc gcaggttgaa ccggaagatc
    13501 tgatcaagtt tggtcttatc cctgagttta ttggtcgtct gccggttgtc gcaacgttga
    13561 atgaactgag cgaagaagct ctgattcaga tcctcaaaga gccgaaaaac gccctgacca
    13621 agcagtatca ggcgctgttt aatctggaag gcgtggatct ggaattccgt gacgaggcgc
    13681 tggatgctat cgctaagaaa gcgatggcgc gtaaaaccgg tgcccgtggc ctgcgttcca
    13741 tcgtagaagc cgcactgctc gataccatgt acgatctgcc gtccatggaa gatgtcgaaa
    13801 aagtggttat cgacgagtcg gtaattgatg gtcaaagcaa accgttgctg atttatggta
    13861 agccggaagc gcaacaggca tctggtgaat aattaaccat tcccatacaa ttagttaacc
    13921 aaaaaggggg gattttatct cccctttaat ttttcctcta ttctcggcgt tgaatgtggg
    13981 ggaaacatcc ccatatactg acgtacatgt taatagatgg cgtgaagcac agtcgtgtca
    14041 tctgattacc tggcggaaat taaactaaga gagagctcta tgaatcctga gcgttctgaa
    14101 cgcattgaaa tccccgtatt gccgctgcgc gatgtggtgg tttatccgca catggtcatc
    14161 cccttatttg tcgggcggga aaaatctatc cgttgtctgg aagcggcgat ggaccatgat
    14221 aaaaaaatta tgctggtcgc gcagaaagaa gcttcaacgg atgagccggg tgtaaacgat
    14281 cttttcactg tcgggaccgt ggcctctata ttgcagatgc tgaagctgcc tgacggcacc
    14341 gtcaaagtgc tggtcgaggg gttacagcgc gcgcgtattt ctgcgctctc tgacaatggc
    14401 gaacactttt ctgcgaaggc ggagtatctg gagtcgccga ccattgatga gcgggaacag
    14461 gaagtgctgg tgcgtactgc aatcagccag ttcgaaggct acatcaagct gaacaaaaaa
    14521 atcccaccag aagtgctgac gtcgctgaat agcatcgacg atccggcgcg tctggcggat
    14581 accattgctg cacatatgcc gctgaaactg gctgacaaac agtccgttct ggagatgtcc
    14641 gacgttaacg aacgtctgga atatctgatg gcaatgatgg aatcggaaat cgatctgctg
    14701 caggttgaga aacgcattcg caaccgcgtt aaaaagcaga tggagaaatc ccagcgtgag
    14761 tactatctga acgagcaaat gaaagctatt cagaaagaac tcggtgaaat ggacgacgcg
    14821 ccggacgaaa acgaagccct gaagcgcaaa atcgacgcgg cgaagatgcc gaaagaggca
    14881 aaagagaaag cggaagcaga gttgcagaag ctgaaaatga tgtctccgat gtcggcagaa
    14941 gcgaccgtag tgcgtggtta tatcgactgg atggtacagg taccgtggaa tgcgcgcagc
    15001 aaggtcaaaa aagacctgcg tcaggcgcag gaaatccttg ataccgacca ttatggtctg
    15061 gagcgcgtga aagatcgcat ccttgagtac cttgcggttc aaagccgtgt caacaaaatc
    15121 aagggaccga tcctctgcct ggtagggccg ccgggggtag gtaaaacctc tcttggtcag
    15181 tccattgcca aagccaccgg gcgtaaatat gtccgtatgg cgctgggcgg cgtgcgtgat
    15241 gaagcggaaa tccgtggtca ccgccgtact tacatcggtt ctatgccggg taaactgatc
    15301 cagaaaatgg cgaaagtggg cgtgaaaaac ccgctgttcc tgctcgatga gatcgacaaa
    15361 atgtcttctg acatgcgagg cgatccggcc tctgcactgc ttgaagtgct ggatccagag
    15421 cagaacgtag cgttcagcga ccactacctg gaagtggatt acgatctcag cgacgtgatg
    15481 tttgtcgcga cgtcgaactc catgaacatt ccggcaccgc tgctggatcg tatggaagtg
    15541 attcgcctct ccggttatac cgaagatgaa aaactgaaca tcgccaaacg tcacctgctg
    15601 ccgaagcaga ttgaacgtaa tgcactgaaa aaaggtgagc tgaccgtcga cgatagcgcc
    15661 attatcggca ttattcgtta ctacacccgt gaggcgggcg tgcgtggtct ggagcgtgaa
    15721 atctccaaac tgtgccgcaa agcggttaag cagttactgc tcgataagtc attaaaacat
    15781 atcgaaatta acggcgataa cctgcatgac tacctcggtg ttcagcgttt cgactatggt
    15841 cgcgctgata acgaaaaccg tgtcggtcag gtaaccggtc tggcgtggac ggaagtgggc
    15901 ggtgacttgc tgaccattga aaccgcatgt gttccgggta aaggcaaact gacctatacc
    15961 ggttcgctcg gcgaagtgat gcaggagtct attcaggcgg cgttaacggt ggttcgtgcg
    16021 cgtgcggaaa aactggggat caaccctgat ttttacgaaa aacgtgacat ccacgtccac
    16081 gtaccggaag gtgcgacgcc gaaagatggt ccgagtgccg gtattgctat gtgcaccgcg
    16141 ctggtttctt gcctgaccgg taacccggtt cgtgccgatg tggcaatgac cggtgagatc
    16201 actctgcgtg gtcaggtact gccgatcggt ggtttgaaag aaaaactcct ggcagcgcat
    16261 cgcggcggga ttaaaacagt gctaattccg ttcgaaaata aacgcgatct ggaagagatt
    16321 cctgacaacg taattgccga tctggacatt catcctgtga agcgcattga ggaagttctg
    16381 actctggcgc tgcaaaatga accgtctggc atgcaggttg tgactgcaaa atagtgacct
    16441 cgcgcaaaat gcactaataa aaacagggct ggcaggctaa ttcgggcttg ccagcctttt
    16501 tttgtctcgc taagttagat ggcggatcgg gcttgccctt attaaggggt gttgtaaggg
    16561 gatggctggc ctgatataac tgctgcgcgt tcgtaccttg aaggattcaa gtgcgatata
    16621 aattataaag aggaagagaa gagtgaataa atctcaattg atcgacaaga ttgctgcagg
    16681 ggctgatatc tctaaagctg cggctggccg tgcgttagat gctattattg cttccgtaac
    16741 tgaatctctg aaagaagggg atgatgtagc actggtaggt tttggtactt ttgccgttaa
    16801 agagcgtgct gcccgtactg gccgcaaccc gcagaccggt aaagagatca ccatcgctgc
    16861 tgctaaagta ccgagcttcc gtgcaggtaa agcactgaaa gacgcggtaa actaagcgtt
    16921 gtccccggtg gggatgtgac gaagttcaag ggcgcatcta ctgatgtgcc ttttttattt
    16981 gtattcggtg actttctgcg tcttgtgggc tgacaattgc ccccgtttct tgtcacaata
    17041 ggcctttgcg cgcatcgata cgttgcgtga ggtacacagt catctacagc ggagtgttgt
    17101 tacaccatga tggacagctt acgcacggct gcaaacagtc tcgtgctcaa gattattttc
    17161 ggtatcatta tcgtgtcgtt catattgacc ggcgtgagtg gttacctgat tggcggaggc
    17221 aataactacg ccgcaaaagt gaatgaccag gaaatcagcc gtgggcagtt cgagaacgcc
    17281 tttaacagcg agcgtaatcg catgcagcaa cagctgggcg atcaatactc cgagctggca
    17341 gcgaatgaag gctatatgaa aaccctgcgt caacaggtgc tgaatcgtct gatcgacgag
    17401 gcgctgttgg atcagtacgc tcgtgagctg aaactgggta tcagtgatga gcaggttaaa
    17461 caggcgattt tcgcgacccc agccttccag gttgacggca aatttgataa cagccgctat
    17521 aacggtatcc tcaaccagat ggggatgacc gccgatcagt acgcccaggc gctgcgtaac
    17581 cagctcacta cccaacagct gattaacggt gttgccggta ccgattttat gctgaaaggt
    17641 gaaaccgacg agctggcggc actggtcgcg caacaacgcg tggtgcgtga ggcgactatc
    17701 gatgttaacg cgctggcggc gaagcagcct gtgaccgaac aggagattgc cagctactac
    17761 gaacaaaaca aaaacaattt catgacgccg gaacaattcc gcgtgagtta catcaagctg
    17821 gatgccgcaa cgatgcagca accggttagc gatgcggata tccagagcta ctacgatcag
    17881 catcaggatc aattcaccca gccgcagcgt acccgctaca gcatcatcca gaccaaaact
    17941 gaagatgaag cgaaagcggt acttgatgag ctgaataaag gcggtgattt tgctgcatta
    18001 gccaaagaaa aatctgccga tattatctct gcccgtaacg gcggcgatat gggttggtta
    18061 gaagatgcca ctatccccga cgaactgaaa aatgctggtc tgaaagaaaa agggcaactg
    18121 tctggtgtca tcaaatcttc ggtcggtttc ctgattgtac gtctggacga cattcagcca
    18181 gcgaaagtga aatcgttaga cgaagtacgt gacgacattg cggcgaaagt gaaacacgaa
    18241 aaagccctcg atgcgtacta tgcgctgcag cagaaagtga gcgatgcggc aagcaacgac
    18301 accgagtctc tggccggtgc agagcaagct gccggcgtta aagccactca gacgggttgg
    18361 tttagcaaag ataacctgcc ggaagagttg aacttcaagc cggttgccga cgctatcttt
    18421 aacggcggtc tggtaggtga aaacggcgcg ccgggcatca actctgacat catcaccgta
    18481 gacggcgacc gcgcattcgt gctgcgcatc agcgagcaca aaccggaagc ggtgaaaccg
    18541 ttggcagatg ttcaggaaca agttaaggca ctggttcagc acaacaaagc tgaacaacag
    18601 gcgaaagtgg atgctgagaa actgctggtt gatttgaaag ccggcaaagg tgcggaagct
    18661 atgcaggctg ccggtctgaa atttggcgag ccgaaaatct taagccgttc cggtcgtgac
    18721 ccgattagcc aggcggcgtt tgcactgcca ctgccagcga aagacaaacc gagctacggt
    18781 atggcgaccg atatgcaagg taatgtggtt ctgctggcgc tggatgaagt gaaacaaggt
    18841 tcaatgccgg aagatcagaa aaaagcgatg gtgcagggta tcacccagaa caacgcacaa
    18901 atcgtctttg aagctctgat gagtaacctg cgtaaagagg cgaaaatcaa aattggcgat
    18961 gcgctggaac agcaataatc ctgaagccgc ctcgcaaaaa aatgcgttgc agctgtaaca
    19021 agaaaaggtc gctttcgcgg ccttttccat ttctgaacat tgccatttgt ttactgtttt
    19081 cactcccgtt aaggtgattc cactgttaac aaacaaggag aaaacagtat gaaacacgga
    19141 attaaagcac tgctcattac cctgtccctg gcctgtgccg gaatgtctca tagcgcgctg
    19201 gcggcagctt ctgtggcgaa accgacggcg gtagaaacca aagcggaagc tcctgcagca
    19261 caaagtaaag cagcagtacc ggcgaaagcc agtgacgaag aaggcacccg ggtcagcatt
    19321 aataatgcca gcgcggaaga gctagcccgc gcgatgaatg gcgttggcct gaagaaagcg
    19381 caggcgattg tcagttatcg cgaagagtac ggtccgttta aaacggtgga ggatctaaag
    19441 caggtgccgg ggatgggcaa ttcgctggtg gaacgtaatc tggcggtatt aaccctgtaa
    19501 ttaatttgca tagtggcaat tttttgccag actgaagagg tcataccagt tatgacctct
    19561 gtacttataa caacaacgta aggttattgc gctatgcaaa cacaaatcaa agttcgtgga
    19621 tatcatctcg acgtttacca gcacgtcaac aacgcccgct accttgaatt tctcgaagaa
    19681 gcccgctggg atgggttgga aaatagcgac agttttcagt ggatgacggc ccataacatc
    19741 gccttcgtcg tggtcaatat caatattaac tatcgtcgcc cagcggtatt aagcgacctg
    19801 ttaactatta ccagtcagtt gcagcaatta aacggtaaaa gcggcatctt aagccaggtc
    19861 attacactgg agccggaagg gcaggtggta gcggatgcgc ttattacgtt tgtttgtatt
    19921 gatcttaaaa cgcagaaagc attagctctg gaaggggaat tgcgcgaaaa gctggagcag
    19981 atggttaagt aaacgttttg tggtgccgga tgctcaagcc gcatccggcg acacccggaa
    20041 taattacctc aacccggttt tctgcttcat cgctgccatc accgtcggtt tatcggccag
    20101 ataatgattc aaaccgttgg cgcgtaaatt acatgccgca caatgaccgc aaccgtcgcc
    20161 tttaatgccg ttatagcagg tcaacgtttc gttacggact aaatccagtt tgccgtaata
    20221 atctgccagc gcccaggttt ccgctttatc aatccacatc agcggcgttt caaaacgaat
    20281 atctttcgcc atgcccaaac tgacggcatg gtttagtgct ttcacaaact catcgcggca
    20341 atccgggtag ccggagaaat ccgtttcaca gacgccggta attacggctt ctgcttttac
    20401 ctgatacgca tatattgccg ccagcgtcag gaacaaaata ttacgccctg ggacaaacgt
    20461 attcgggatg ccatcggctt caggttcata atcaggcacc ggaatgctgt cacgtgtcag
    20521 gctgctgacc gccagctcgt tgagcagcgt tacatcaaga accttatgcg cgcgtgctcc
    20581 cagtttcagc gccagttcgc gtgccacgtc gatttctgcg cgatgtcgct gaccgtaatc
    20641 gaacgtcacg caatggactt catcatattg ttgtaatgcc tgcaccagac aggtggtgga
    20701 atcctggcct ccactgaaca caacgacagc acgtttcata gataatcctg cttgaacaat
    20761 aaaagcgtta tggtaacgcc tgcgattaac ccggaccagc tattcattgc gacggcgcgg
    20821 gaagccaggc ttcggtaaat tcaaaccagc cgcgcggtgt cagtcgcaca ccgttcacgc
    20881 cgggaggggc actaatgcga tagtgatagt tgaacagcgg cgtcagcgtc gcgtctgcca
    20941 ttaactggct aaaaaccgct ttcagggcat taaatcggtt ttcctcatca ggcattactt
    21001 gcaccgcgtc cagcgtcgat tgcagatgtg catatgctgg agcgtcgaaa acatgtggcc
    21061 atagtggatc gcaacgtagc cattgctcca gagtatattc cggtgcttcg ccaattaatc
    21121 tgtcgcccat catgaggtct gcgtgtgcca atagggtcgt gtcgtcccag ttttttgcgt
    21181 tatgaaaaat aattgtaagt tcacagcctt ccgctgccag tgtcgcctgt aggcgttctg
    21241 ccatggtatg aagttctatc ggtaggtgat aaaccagcgt caaggttttc ggtagtttga
    21301 cttcatccgg tacttgccat tgcggaatag tccagcctgg cagtaatgca tgactggcgg
    21361 tgatcaggtt ttctcctact tctaacgttt gtaataaacc ggattgatga ataatggaga
    21421 tcacttttcg cgcctgccag agggagagtc ggggactttt gcgcaacgtt aaatagcaaa
    21481 aacctaaact gatgccacta ctgacctggc tgacccgttg cagctcctcc ggtttgccga
    21541 tggtgatttg cacgggatgc cgacagctgg ttcccaaatc tttttcgaaa agcggcggag
    21601 ttatccagta ctcaaccgct ttaagcagtg gatgacgtaa atggtaataa tcatggcttt
    21661 ccaggcgcac cagctccgct gtgaattgtg ttaagcgaaa aggacccgtg ccgatcagtg
    21721 ggaattgcgg atgcgccaga tggctacaat agctcgccag ccggtgcgca agccagtaat
    21781 cagggcgatg taaaaagaag gtcagacact gcggatgggt gacttcaata cgcttcacgc
    21841 taataaataa ttgatccagt gctggcagtt gtaacagcat caataatcgc tggtgtaagt
    21901 gtgaggcttt tactgcatcg ccgttatgcc agtgtagggt tgaacgaaga taaaagtccc
    21961 agcgtaaccc gtcagtagag gtttcccagt gatgcgctaa atcgccaatc gggcgctggg
    22021 tattattatc gaagcgggtc aggccggaaa atatctgtcc ggcgagatgc tgctcggcac
    22081 ggccgggcaa aaagcctggt tgtagcggtt cgagcgggcg atagtaggga atacgcaacg
    22141 tgggtgtatc gttttgccat tgtccgccca taaacggctg taacagagtg cgcagctcac
    22201 ctggggccag ttgcgccagc tccagcacat cttgctgctt tccggtttcc agtgcctgtt
    22261 ccatcatcgc attgcgtagc gattccggcg tgaccagaaa gcgtaattgt ccgcgctttc
    22321 cgcgtcctga ctgcgcctgc cactccagcc atcccgcctc ctgtgcctga cgcaacagcg
    22381 tacgaacatg gcgttcgctg caaaaacagc gttcggccag ttcattgacg gtgacagctt
    22441 gcggctttcc ggcggaaggt tgccacagac gttgatactg gttaagacgg ttgagcaatc
    22501 gcatataaac ccggaacaat attatttaac tattcactat tacttccgta tatatcaggt
    22561 gatactcaat caccattaac cgtgtcacag agtggagaaa gaaatggctc gtctggcagc
    22621 atttgatatg gatggcactt tattgatgcc tgaccatcat ttaggtgaga aaaccctctc
    22681 tactttggcg cgactgcgtg aacgcgacat taccctcact tttgccacgg ggcgtcatgc
    22741 gctggagatg cagcatattc tcggggcgct atcgctggat gcatatttga ttaccggcaa
    22801 cggaacgcgc gtgcattctc tggaaggtga acttttacat tgtgatgatt tacctgcgga
    22861 tgtcgcggag ctggtgctgt atcagcaatg ggatacccga gccagcatgc atatcttcaa
    22921 tgacgacggt tggtttaccg ggaaagagat ccctgcgttg ttgcaggcat ttgtctatag
    22981 cggttttcgt tatcagataa tcgatgtcaa aaaaatgcca ctcggcagcg tcaccaagat
    23041 ctgcttctgt ggcgatcacg acgatcttac acgcttgcag atccagctat acgaagcatt
    23101 aggcgagcgt gcacatttgt gtttttccgc cacggattgc ctcgaagtgc tgccggtggg
    23161 ctgcaataaa ggcgctgcat tgacggtgct gacccaacat ttaggtttat cgttgcgcga
    23221 ttgcatggcc tttggtgatg cgatgaacga tcgcgaaatg ttaggcagcg tcggtagcgg
    23281 ctttattatg ggcaatgcga tgccgcaact gcgcgcggag ctcccgcatt taccggtgat
    23341 tggacattgc cgaaatcagg ctgtctctca ctatttgacg cactggctgg actatccaca
    23401 tctaccttat tcccccgaat aacgagatcc cttccagcac cgggcaattg cccggttttt
    23461 tttgcgttga atttgtcatt ttgtgccgtg gtgtttaaac cgcacagaat aaattgtcgc
    23521 gatttcacct ttaaaataga attaaaagag aaaaaaattc tccgtggaag ggctatgtta
    23581 gataaaattg accgtaagct gctggcctta ctgcagcagg attgcaccct ctctttgcag
    23641 gcactggctg aagccgttaa tctgacaacc accccttgct ggaagcgcct gaaacggctg
    23701 gaggacgacg gtatccttat cggcaaagtc gccctgctgg atccggaaaa aataggcctc
    23761 ggcctgaccg cctttgtgct gataaaaacg caacatcaca gcagcgaatg gtattgccgc
    23821 tttgtcacgg tggttaccga aatgccagaa gtgctggggt tctggcgcat ggctggtgaa
    23881 tacgattatc tgatgcgcgt ccaggttgcc gacatgaaac gctacgacga gttttataag
    23941 cgtctggtaa acagcgtgcc ggggctgtcg gacgtcactt ccagcttcgc gatggaacag
    24001 attaaataca ccacttcttt acccatcgaa taaatatcca gaatcaggtc aggacacaac
    24061 gcgtgcgatt atttgctcaa ttaagctggt atttccgtcg ggaatggcgt cgctatctcg
    24121 gggctgtcgc cttgcttgtc attatcgcga tgctgcaact ggttccgcca aaagtggttg
    24181 gtattgttgt cgatggcgtg acagaacaac actttactac cgggcagatc ctgatgtgga
    24241 tcgccaccat ggtgctgatt gccgttgtgg tttatctcct gcgttacgtc tggcgggtat
    24301 tgctgtttgg tgcgtcttat caactggctg ttgaactgcg tgaagattat taccgtcagc
    24361 taagccggca gcatcctgag ttttacctgc gtcatcgcac gggtgacctc atggctcgtg
    24421 cgactaatga cgtcgatcgc gtcgtgtttg ccgccggaga aggggtgctg acgctggtgg
    24481 attcactggt aatgggctgc gctgtgttga ttatgatgtc tacgcaaatt agctggcagt
    24541 tgaccttatt tgccctgtta ccgatgccag tgatggcgat catgattaag cgcaacggcg
    24601 atgccttgca tgaacgcttt aagctggcac aggcggcgtt ttctagtctt aatgaccgca
    24661 cccaggaaag cctcaccagt attcgcatga tcaaagcctt tggtctggaa gatcgccagt
    24721 cggcgttatt tgccgcggat gccgaagata ccggcaaaaa aaacatgcgg gtggcgcgta
    24781 ttgatgctcg tttcgacccg accatctata tcgcgattgg tatggcgaac ttgctggcga
    24841 ttggcggcgg tagttggatg gtggtgcagg gcagtttaac gctgggccag ctcaccagtt
    24901 ttatgatgta tttaggtctg atgatttggc caatgctggc gctggcatgg atgtttaaca
    24961 ttgtggaacg tggtagtgct gcgtacagcc gtattcgcgc gatgctggcg gaagcgccgg
    25021 tggtgaacga tggtagcgaa cccgtgccgg aagggcgtgg cgaactggat gtaaatattc
    25081 accagttcac gtatccgcag actgaccatc ctgcgctgga aaacgtcaat ttcgccctga
    25141 aacccggtca gatgctgggt atttgcgggc cgactggttc cggcaaaagt accctgttgt
    25201 cgctcattca gcgtcatttc gacgtcagcg agggggatat tcgctttcat gatattcctc
    25261 tgacgaagtt acaactcgat agctggcgta gccgcctggc ggtggtcagc cagacgccat
    25321 tccttttttc tgacaccgtg gcgaataaca tcgcgctggg ttgcccgaat gccacccagc
    25381 aagagattga acacgtagcg cggttagcca gcgtacatga cgatattttg cgtctgccgc
    25441 aaggttacga tacagaagtg ggcgagcgcg gcgtgatgct ttccggtggg caaaaacagc
    25501 gtatctccat tgctcgagcg ttattagtca acgcggaaat cctcatcctt gatgatgcgc
    25561 tttcggcggt ggacggacgc acagagcacc agatcctgca taacctgcgt cagtggggac
    25621 agggaagaac ggtaatcatc agtgcccatc gcctttcggc actgacggaa gccagtgaaa
    25681 ttattgtgat gcagcacgga catatcgccc agcgtggcaa tcatgatgag ctggcgcaac
    25741 aaagcggctg gtatcgcgat atgtatcgct atcaacaact ggaggcggcg ctcgacgacg
    25801 ttccggaaat tcgcgaggag gccgtcgatg cgtagtttta gccaactgtg gccgactctc
    25861 aagcgtctgt tagcgtacgg ttcgccgtgg cggaaaccgc tggggattgc ggtcctgatg
    25921 atgtgggttg cggcagcggc agaagtcagt gggccgctgc ttatcagcta ttttatcgac
    25981 aatatggtag cgaaaaataa cctgccgttg aaaatggttg cagggctggc ggcggcgtat
    26041 gttgggctac aactgtttgc cgccgggcta cattacgcgc agtcgctgct gtttaatcgg
    26101 gcggcagtag gcgtagtgca acagttgcgt accgacgtga tggatgctgc gttacgccag
    26161 ccattaagcg agtttgatac ccaacccgtc gggcaggtga tttcccgcgt cactaatgac
    26221 actgaagtga tccgcgatct ctacgttacc gtagtggcaa ctgtcctgcg tagcgccgca
    26281 ctggtgggcg cgatgctggt ggcaatgttc agcctcgact ggcgaatggc gctggtggcg
    26341 ataatgattt tcccggtagt gctggtggta atggtgatat atcaacgtta cagcacgccg
    26401 attgtccgtc gtgtgcgcgc ctatctggcg gatatcaacg acggctttaa cgaaatcatc
    26461 aatggcatga gcgttatcca gcagttccgt cagcaggcgc gatttggcga acgtatgggt
    26521 gaggccagtc gttcacacta tatggcgagg atgcaaaccc tgcgcctcga cggttttctg
    26581 ctgcgtccgc tgctgagtct gttttcatcg ctcattcttt gtggcttgtt gatgctgttt
    26641 ggcttctccg ccagcggcac cattgaagtg ggcgtgttat atgcgtttat cagctatctt
    26701 gggcgactta acgaaccctt aatcgaactg accacgcagc aggcgatgct gcaacaggct
    26761 gttgttgctg gtgagcgcgt gtttgaactg atggacggac cgcgccagca atatggcaat
    26821 gatgatcgcc cgttacagag cggcaccatc gaagtcgata acgtgtcatt tgcttatcgc
    26881 gatgacaatc tggtgctaaa gaacattaat ctctctgtgc cttcgcgtaa ctttgtggcg
    26941 ctggtcgggc ataccggcag tggcaaaagc accctcgcca gtttattgat gggctattac
    27001 ccgctaacgg aaggtgagat tcgccttgat ggtcgtccat taagttcgct aagtcacagc
    27061 gcgctgcgcc agggcgtggc aatggtgcag caagatccgg tggtgctggc ggataccttc
    27121 ctcgccaacg tgacgctggg acgtgatatc tccgaagaac gcgtctggca ggcgctggaa
    27181 accgtgcaac tggcggagct ggcgcgcagc atgagtgatg gtatttatac gccgctgggc
    27241 gagcagggaa ataatctctc agtcgggcaa aagcaactgc tggcactggc gcgcgtgctg
    27301 gtggagacgc cgcaaatcct gatccttgat gaggcaaccg ccagcattga ctccggtact
    27361 gaacaggcga ttcaacatgc tctggcggcg gtgcgtgaac ataccacgct ggtggtgatt
    27421 gctcaccgct tatcaactat tgttgatgcc gacaccattc tggtgcttca tcgtgggcaa
    27481 gccgtggagc agggcactca ccagcaactg ctggcggccc agggacgcta cttgcagatg
    27541 tatcaactgc aacttgcggg cgaagagctg gcagccagcg tgcgtgaaga ggaatcattg
    27601 agcgcctgaa tagcgcaata tttcatcgtt ggtgcaaaaa tgtaacgcac tgtgcactgt
    27661 catagtgcgt tttcattttc aatcttctta acttcctgct ctctttctcg tttttcattt
    27721 ctggcacacc gcttgcaata ccttcttcgt gtagcagaac cattaccgaa ttctgaccgg
    27781 aggggatcta tgaagctggt gaccgtgata atcaaaccat tcaagctgga agacgttcgt
    27841 gaagcgttat cttccattgg tattcagggc ctgaccgtca ccgaagtgaa aggtttcggg
    27901 cgtcagaaag ggcatgccga gctgtaccgg ggggcggaat acagcgtcaa cttcctgcca
    27961 aaagtaaaaa ttgatgtgtc gattgctgat gaccaactcg atgaagtgat cgatatcgtc
    28021 agtaaggcgg cttacaccgg aaaaattggc gacggcaaaa tcttcgtcgc tgaattgcaa
    28081 cgcgtcattc gtattcgtac cggcgaagcc gacgaagcgg cgctgtaatc tctggcacac
    28141 agcaacagga acgaaaaatg aagatagcga cgataaaaac tgggcttgct tcactggcga
    28201 tgctgccggg actggtaatg gctgcacctg cggtggccga taaagccgac aatgcgttta
    28261 tgatgatttg tactgcgctg gtgctgttta tgactattcc ggggattgcc ctgttttacg
    28321 gtgggttgat tcgcggcaaa aacgtgctgt cgatgctgac gcaggtgacg gtgacgtttg
    28381 cactggtctg tattctctgg gtggtttacg gttactcgct ggcgtttggt gagggcaaca
    28441 acttcttcgg caacattaac tggttgatgc taaaaaacat cgaactgacg gcggtgatgg
    28501 gcagcattta tcagtatatc cacgtggcgt ttcagggctc gtttgcctgc attaccgtcg
    28561 gcttgatcgt tggggcgctg gcggaacgaa tccgcttctc agctgtgttg attttcgtgg
    28621 tggtatggct gacgctctct tacattccga ttgcgcatat ggtgtggggc ggtggtttgc
    28681 tggcttctca cggtgcgctg gatttcgcgg gtggcacggt ggtgcatatt aacgccgcaa
    28741 tcgccggtct ggtgggcgcg tatctgatag gcaaacgcgt gggcttcggc aaagaggcgt
    28801 ttaaaccgca caacctgcca atggtcttca ccggtactgc cattctctat attggttggt
    28861 ttggctttaa cgccgggtca gcgggcacgg cgaatgaaat cgcggcactg gcatttgtga
    28921 atactgtggt cgcaacggcg gcggcaattc ttggctggat cttcggtgaa tgggcgctgc
    28981 gtggtaagcc ttctctgctg ggagcgtgtt ctggcgcgat tgccggtctg gtcggcgtga
    29041 cgccagcgtg tggctacatt ggggttggcg gcgcgttgat tatcggcgtg gtagctggtc
    29101 tggcgggctt gtggggcgtt actatgctca aacgcttgct gcgggtggat gatccctgcg
    29161 atgtcttcgg tgtgcacggc gtttgtggca ttgtcggctg tatcatgact gggatttttg
    29221 ccgccagttc gctgggtggc gtgggcttcg ctgaaggtgt gacgatgggc catcagttgc
    29281 tggtacagct ggaaagcatc gccattacga tcgtctggtc cggtgtggtg gcatttattg
    29341 gctacaaact ggcggatctg acggttggtc tgcgtgtacc ggaagagcag gagcgagaag
    29401 ggctggatgt caacagtcac ggcgagaatg cctataacgc gtaacaagca ctgcaaaaaa
    29461 cagccggacg gttttcacct ccggctattt ttttaattgt gattacgcat caccccttcc
    29521 tgaacggtcg aggcaaccag tacgccgtcc tgggtataaa actcaccgcg cacaaagcca
    29581 cgtgcgctgg acgccgaggt gctctccacg ctatacagca gccattcatt caaattaaac
    29641 gggcgatgga accacatgga atggtcaatg gtggcaatct gaatccccgg ttcgagaaaa
    29701 ccgatgccgt gcggctgtag agctaccggc aggaagttaa gatcagaagc gtaaccgagc
    29761 agatactgat gaacgcgcag gtcatccggc acgctaccat ttgcgcggat ccacacctga
    29821 cgatgtggtt ctgcgacgtg acctttcagt gggttatgaa actccaccgg acggacttcc
    29881 agcggacgat cgcagatgaa tttatcttta agcactggcg gcagcaggtg cgccagcgat
    29941 tgggcgattt gcgtttccga aggcaggcca tcaggcgctg gcgcggacgg cattgttttt
    30001 tgatgttcga aacccgcttc tggtgcctgg aaagaggccg tcatataaaa aatcggtttg
    30061 ccgttttgaa tagcagcaac ccggcgggcg ctgaagctgt taccgtcacg cagcgtttcg
    30121 acatcataaa taatcggctt cttactatcg ccagggcgaa gaaagtagct gtgaaacgaa
    30181 tgtaccagcc gctcttcagg gacggtctct tttgcagcat acaaggcctg acccacgacc
    30241 tggccgccaa acacctggcg taaacctaaa tcttcactct ggccgcgaaa gagtccttcc
    30301 tcaatttttt ccagatttaa caatgtcagt aaatttttta gcgcctgact catataactc
    30361 tccagtaaca aagctgccgc agcaagccaa agtgagttga gtataacgca aatttgctac
    30421 tggtccgatg ggtgcaatgg tctgaattac gggctaatta caggcagaaa tgcgtgatgt
    30481 gtgccacact tgttgacgtt actattttgt taaccactct tccggcgagg aaagttagcc
    30541 cgctggtgca ttgataataa ggagaaatga atgaaactcg tgcacatggc cagtggttta
    30601 gcggttgcga ttgcgttggc ggcttgcgca gataaaagcg cggatattca gacgccagcc
    30661 ccggctgcaa atacgtctat ttcagcaaca caacaacctg ctatccagca accgaatgtc
    30721 tccggtaccg tctggatccg gcagaaagtc gcactgccgc ctgatgctgt gctgaccgtg
    30781 acactttctg acgcgtcgtt agccgatgca ccgtcaaaag tgctggcgca gaaagcggtg
    30841 cgtaccgaag gtaaacagtc accattcagc tttgttctgc catttaatcc ggcagatgtt
    30901 cagccgaacg cgcgtattct gttgagtgcg gcgattaccg tgaatgacaa actggtattt
    30961 atcaccgata ccgttcagcc ggtgatcaac cagggcggaa ctaaagccga cctgacattg
    31021 gtgccggtac agcaaaccgc cgtgccggtt caggccagcg gtggtgcaac gactaccgta
    31081 ccttcgactt caccaactca ggtgaatccg tcttcggcag ttccagctcc tacgcaatat
    31141 taagcgacgt taaccctctc cgcccggaga gggttagtag ttccagcgat aacgctgcaa
    31201 gtcgatttgc ccgcttcccg ataccatcac accttctgcc agtaatgcct gtcgctgacg
    31261 ctgtaaatcc ggtccggtta gcgaaattgt gccgtggcga ttaaccaccc ggtgccaggg
    31321 taaggtgctg ccttcaggga gacgctttaa cacaccgccc acctggcgcg cggcgcgggg
    31381 cgatcccgcc agtttcgcca catcaccgta agtggtgaca tagccttcgg gaatagcggc
    31441 gacgatttgc cagacgcgtt ggggaaatga atcttctttt tccatctttt cttcctgagg
    31501 taatttttca gcataatctg gaaaaacgcc cgagtgaagt cgcattgcgc aagaaaccag
    31561 catctggcac gcgatgggtt gcaattagcc ggggcagcag tgataatgcg cctgtgcgtt
    31621 ggttctcaac gctctcaatg ggggctctgt tggttctccc gcaacgctac tctgtttacc
    31681 aggtcaggtc cggaaggaag cagccaaggc agatgacgcg tgtgccggga tgtagctggc
    31741 agggccccca cccatttctg cctcccaccg tttcgtcaaa aaaccccaac atggctaaac
    31801 tttaaccaca actgacgtcg caagaattgt ctggctgcgc agtacgcttc ggaggtatgt
    31861 ctgatgaagt atgttgatgg ttttgtggtt gccgttcctg ccgataaaaa ggatgcctat
    31921 cgggaaatgg ccgctaaggc tgcgccattg tttaaagagt ttggcgcgct tcgtattgtc
    31981 gaatgctggg ccagcgatgt accggatggc aaagtgaccg attttcgtat ggcggtgaaa
    32041 gcggaagaga atgaagaggt tgtctttagc tggattgaat acccttcaaa agaggtccgc
    32101 gacgctgcta atcaaaagat gatgtcggac ccacggatga aagagttcgg cgagtccatg
    32161 ccgtttgacg gcaagcgaat gatctatggc ggattcgagt caatcatcga cgaataggta
    32221 gcgtgacggg ctgtgcggcg tgccgtcagc ccaaaaattc acaaatgttg ctcggcccag
    32281 cgtaagaaat cttctttcgg taatgccttg ctgtagagcc agccctgacc gtagtgcacg
    32341 ccatgctggc gtagccactc ttcttgttta ctggtttcga ttccctccgc taccattttc
    32401 agtttcagtg tttttgccat ttcgatgata tgcggcgtga catttttata ttccagcgca
    32461 tcaacgaaag atttatcgat cttcagaatg tcgacatcca gatcctgtaa ataacttaaa
    32521 cttgaatatc ccgtaccaaa atcatcaaga taaatttcat ggcccgcctc ccggtagcga
    32581 gaaattatcg gggcgctggt tttcggatcg gcaaactcgc gttcagtgag ttcaagcgcg
    32641 atctgtctgg gattaacctg atagtgattg atcatttcac gcagcaattg cgggattttt
    32701 tccgaggtga gcacggtgga ttcaagattg atcgaaatat gctgctgtgg atgctgacgc
    32761 agccagtcgc ccatatcttc aaagacgctt cttataatca gtagcgtcaa tggctcagaa
    32821 aggcccgttt gctgtgccag cggaataaaa ctatctggtg acaaccaact accgtctgtc
    32881 tgcggccagc gcgccagtgc ctcagcaccg acaattttgc cattggctaa ggagacaatc
    32941 ggctgatagt gcacgcaaat atcacgattt tcgatagcat cctgcagccg atgatgcggt
    33001 gactggatac ggcgcaaaat acgcagcaca aacatcgctg ccagcaggcc aatcaccaac
    33061 ccgagcggta accaaataaa gacttgccga tgccagcctt tctgcaacat tttcgttgaa
    33121 gcccaggtga tgatcgaaat attcatctcc gggaagggca ggatatcgta aatgattcca
    33181 ttattttcga tatgctcacc gggtgttttt tgtagcctgg taataattcc ctgagcaatt
    33241 tcatcgctgc tggttatgac aacgttatgg gcattgccaa taatggcggc atcaatttgc
    33301 catgagctat agggaatgac atcaataaag gaagcggggt cgatcatgac gacataatgt
    33361 gccgttccca tggcgaccat gtaacggata atgcctaaat cgttatgcga tgttaaccag
    33421 acacgataac catctttcga aattttacct ggctcgggga aggtatcggg cggactttca
    33481 tgctccagag acgaacactg gggaacgttg ttatcgatat aaaccacttc ctgaatatag
    33541 cgataactgt aagatacccg acgcatttcc atgagatggg cttcgctaca ggcagcgcct
    33601 tgccatcttt ccagctcttg cagcgcatct ttcccttgtg tcgccacctt attggcccga
    33661 atagcgacgc gggaggaata ggtatccagc tcttcaataa acgatgtttc tacctgctga
    33721 tgggccagcc agatgcttaa gccgacaggc agcaatactg aaagaatcag tactcccgaa
    33781 ataaggccga ccagatgtcg tgttctcacc agcatatcct tattaacaca gtgtagggaa
    33841 agtatatctg attgtggaaa aaggctggca ggagataagg aggtagagct ggtatttcat
    33901 gaagtgatgg gtggaaacga tcattgtcat ttgaccaggc gcagagcttt acgcctggta
    33961 tgtaacccta acttaagatg ttgattcggc gcgagcgagg gtaaaaatat cgtaaaaaga
    34021 cagttcacct ttacggacga tcattttttg cagttgttcc ttatgaacat ccgcgacacg
    34081 cggagagttc aagatggctt caaccagcgc ggcaggtaca aagcgcgtgt tgtaccattc
    34141 gccgttatag caaacgcgga aatccagcac gtcgatatct tcataacggt agcgtgggta
    34201 gacatcgaaa aagaagaaac cattaagcag gataaataat acaaccagta gccagacaga
    34261 gcctgacagc gtttcggact gcaacatcac cgccagggtg gcaaaaaaag caacgtacat
    34321 accgataaac agccccggat gtttacggat aaaactgata ctaaagcggg gtttgttgtc
    34381 gcgtttttcg cgggtattca gagactcaat cgtttcggtc agcaggcgtt gtatttcggt
    34441 catcgtttgc cttcgtggta tctgcaactt tccagggaac accctatttt aacgtgggaa
    34501 tagtagaggg gaagtaacag atttaccaca attttgcata acagttgcga aaaaccgatt
    34561 acaatttttt aattattctg gctggattac cgcccacgac aacgttgttc gggacatctt
    34621 ttgtgacaac tgcacctgag gctaccacga cgttatcacc aatggtcaca ccagggttaa
    34681 tgaccgcgcg tccgccaatc cagacgttat taccgatggt gacgggtttc cccagttcag
    34741 caccgctatt acgtgctaca gggtcgatgg gatgtgttgc cgtgtagata tgaacgcctg
    34801 gtgccaacat acagttatca ccgatgcgaa tagggcagac atcaagcatc acgcaatcga
    34861 agttggcgaa aaaattatta ccgagaaaaa tgttatagcc atagtcacag cgaaacgttg
    34921 gctcaatata agcctctgtc acctgaccga atagatcagc gagaatttgc tggcgtaatg
    34981 tgtgctcttc cgccagggaa tgattgtatc ggtgaataag ctgacgagcg cgcaggcgat
    35041 cgcgagataa cgtctcatct gccgagcgat acaactcacc agcaatcatc ttttcttttt
    35101 ctgtgctcat tactgacctc cggttgctga aaatgcggca acacagtaag acgaaaagtc
    35161 agtgaagaga acgttcccat tacatctgtg tgataaagat cacatagggt atgtcttcat
    35221 ggcgaaaaag tataaaattc ttaataaaca gccggttata gctccgaaag cgaattattt
    35281 attagcgaat aaatttccat actgaggaag ggatcttgtc gtacagttta ttcatggtca
    35341 attcggcgag gcggtgatct gcggctgagt aaaataccgc cagttcatta tctgataatt
    35401 cgtatttatt tttctcgata acacgctcca gcgtgtcaat tgtctggcaa cgacgtaaac
    35461 gcattaaata atcggttttc gttaaaggtt tttcggacat acttctaccc atggttgtaa
    35521 taattctaac aagataaact cgcaggattc tctttcgtcg cattgacaaa acaacggaat
    35581 agtcgattac ctgacttccg ccatttctga agatcctgca tattaatacc ataactactg
    35641 aacaacataa aggtgtcatc cagatattcg tcgatctgct caatgagctt attgtcttca
    35701 ttatacttaa ttttgtaatt aagtgcgaag gtcgcaatat gctcaatcag ttcattcaac
    35761 tggaggttga tcgccgaggt tgggtcgtta acccagccat gattgctttc ttcaaggttt
    35821 gcaaggcagt catgatacag ggtttcacag agaaacttaa gctgtgcgat atcatgtctt
    35881 ttgggtgagt attcatccat aacgcgtccc cttcttagcg gttgaactaa cggacacctt
    35941 tcgggatgga aaaaacttac tgacctggac ttgccctctc gtttgttagc ttaactataa
    36001 gccactcttt gcaggttttc atcgcattat tacgaaaaat tacaattagg aaggaatcgg
    36061 gacaggactc actttttcat actagcacta acccgcagca ggtaaaagca gtctgctcga
    36121 actgttcata acatttcagg attatcgcca gatgaaattg gtgacactaa taccaggatt
    36181 gctctgaata tgacgtaata accgaggaa
//
LOCUS       DAZFRN010000036        35252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFRN010000036 DAZFRN010000000
VERSION     DAZFRN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.036"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..89)
                     /locus_tag="ACHKUB_001713"
     CDS             complement(<1..89)
                     /locus_tag="ACHKUB_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 domain-containing protein"
                     /protein_id="HFO6654744.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESAL"
     gene            complement(216..947)
                     /gene="rlmB"
                     /locus_tag="ACHKUB_001714"
     CDS             complement(216..947)
                     /gene="rlmB"
                     /locus_tag="ACHKUB_001714"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO6654745.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1127..3568)
                     /gene="rnr"
                     /locus_tag="ACHKUB_001715"
     CDS             complement(1127..3568)
                     /gene="rnr"
                     /locus_tag="ACHKUB_001715"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO6654746.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3607..4032)
                     /gene="nsrR"
                     /locus_tag="ACHKUB_001716"
     CDS             complement(3607..4032)
                     /gene="nsrR"
                     /locus_tag="ACHKUB_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO6654747.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4237..5535)
                     /gene="purA"
                     /locus_tag="ACHKUB_001717"
     CDS             complement(4237..5535)
                     /gene="purA"
                     /locus_tag="ACHKUB_001717"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO6654748.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5639..5836)
                     /gene="yjeT"
                     /locus_tag="ACHKUB_001718"
     CDS             complement(5639..5836)
                     /gene="yjeT"
                     /locus_tag="ACHKUB_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO6654749.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5918..6922)
                     /gene="hflC"
                     /locus_tag="ACHKUB_001719"
     CDS             complement(5918..6922)
                     /gene="hflC"
                     /locus_tag="ACHKUB_001719"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO6654750.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6925..8184)
                     /gene="hflK"
                     /locus_tag="ACHKUB_001720"
     CDS             complement(6925..8184)
                     /gene="hflK"
                     /locus_tag="ACHKUB_001720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO6654751.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8270..9550)
                     /gene="hflX"
                     /locus_tag="ACHKUB_001721"
     CDS             complement(8270..9550)
                     /gene="hflX"
                     /locus_tag="ACHKUB_001721"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO6654752.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9626..9934)
                     /gene="hfq"
                     /locus_tag="ACHKUB_001722"
     CDS             complement(9626..9934)
                     /gene="hfq"
                     /locus_tag="ACHKUB_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO6654753.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10020..10970)
                     /gene="miaA"
                     /locus_tag="ACHKUB_001723"
     CDS             complement(10020..10970)
                     /gene="miaA"
                     /locus_tag="ACHKUB_001723"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO6654754.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10963..12810)
                     /gene="mutL"
                     /locus_tag="ACHKUB_001724"
     CDS             complement(10963..12810)
                     /gene="mutL"
                     /locus_tag="ACHKUB_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO6654755.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASQDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12820..14157)
                     /gene="amiB"
                     /locus_tag="ACHKUB_001725"
     CDS             complement(12820..14157)
                     /gene="amiB"
                     /locus_tag="ACHKUB_001725"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO6654756.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14176..14637)
                     /gene="tsaE"
                     /locus_tag="ACHKUB_001726"
     CDS             complement(14176..14637)
                     /gene="tsaE"
                     /locus_tag="ACHKUB_001726"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO6654757.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14609..16156)
                     /gene="nnr"
                     /locus_tag="ACHKUB_001727"
     CDS             complement(14609..16156)
                     /gene="nnr"
                     /locus_tag="ACHKUB_001727"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO6654758.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16155..17294
                     /gene="queG"
                     /locus_tag="ACHKUB_001728"
     CDS             16155..17294
                     /gene="queG"
                     /locus_tag="ACHKUB_001728"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO6654759.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17277..17330)
                     /locus_tag="ACHKUB_001729"
     CDS             complement(17277..17330)
                     /locus_tag="ACHKUB_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654760.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17564..17639)
                     /locus_tag="ACHKUB_001730"
     tRNA            complement(17564..17639)
                     /locus_tag="ACHKUB_001730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17604..17606),aa:Gly,seq:gcc)
     gene            complement(17675..17750)
                     /locus_tag="ACHKUB_001731"
     tRNA            complement(17675..17750)
                     /locus_tag="ACHKUB_001731"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17715..17717),aa:Gly,seq:gcc)
     gene            complement(17787..17862)
                     /locus_tag="ACHKUB_001732"
     tRNA            complement(17787..17862)
                     /locus_tag="ACHKUB_001732"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17827..17829),aa:Gly,seq:gcc)
     gene            complement(18073..18618)
                     /gene="orn"
                     /locus_tag="ACHKUB_001733"
     CDS             complement(18073..18618)
                     /gene="orn"
                     /locus_tag="ACHKUB_001733"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO6654761.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18713..19765
                     /gene="rsgA"
                     /locus_tag="ACHKUB_001734"
     CDS             18713..19765
                     /gene="rsgA"
                     /locus_tag="ACHKUB_001734"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO6654762.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19862..20830
                     /gene="asd"
                     /locus_tag="ACHKUB_001735"
     CDS             19862..20830
                     /gene="asd"
                     /locus_tag="ACHKUB_001735"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO6654763.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGEKDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20852..24175
                     /gene="mscM"
                     /locus_tag="ACHKUB_001736"
     CDS             20852..24175
                     /gene="mscM"
                     /locus_tag="ACHKUB_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO6654764.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24326..25828)
                     /gene="yjeM"
                     /locus_tag="ACHKUB_001737"
     CDS             complement(24326..25828)
                     /gene="yjeM"
                     /locus_tag="ACHKUB_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO6654765.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTLHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(26047..27024)
                     /gene="epmA"
                     /locus_tag="ACHKUB_001738"
     CDS             complement(26047..27024)
                     /gene="epmA"
                     /locus_tag="ACHKUB_001738"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6654766.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27349..29157
                     /gene="frdA"
                     /locus_tag="ACHKUB_001739"
     CDS             27349..29157
                     /gene="frdA"
                     /locus_tag="ACHKUB_001739"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6654767.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29150..29884
                     /gene="frdB"
                     /locus_tag="ACHKUB_001740"
     CDS             29150..29884
                     /gene="frdB"
                     /locus_tag="ACHKUB_001740"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6654768.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29895..30290
                     /gene="frdC"
                     /locus_tag="ACHKUB_001741"
     CDS             29895..30290
                     /gene="frdC"
                     /locus_tag="ACHKUB_001741"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6654769.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30301..30660
                     /gene="frdD"
                     /locus_tag="ACHKUB_001742"
     CDS             30301..30660
                     /gene="frdD"
                     /locus_tag="ACHKUB_001742"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6654770.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            30723..31856
                     /gene="ampC"
                     /locus_tag="ACHKUB_001743"
     CDS             30723..31856
                     /gene="ampC"
                     /locus_tag="ACHKUB_001743"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO6654771.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPTWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            31945..32478
                     /gene="blc"
                     /locus_tag="ACHKUB_001744"
     CDS             31945..32478
                     /gene="blc"
                     /locus_tag="ACHKUB_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6654772.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32475..32792)
                     /gene="sugE"
                     /locus_tag="ACHKUB_001745"
     CDS             complement(32475..32792)
                     /gene="sugE"
                     /locus_tag="ACHKUB_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6654773.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(32967..33113)
                     /gene="ecnB"
                     /locus_tag="ACHKUB_001746"
     CDS             complement(32967..33113)
                     /gene="ecnB"
                     /locus_tag="ACHKUB_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6654774.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33224..33349)
                     /gene="ecnA"
                     /locus_tag="ACHKUB_001747"
     CDS             complement(33224..33349)
                     /gene="ecnA"
                     /locus_tag="ACHKUB_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6654775.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33401..33967)
                     /gene="efp"
                     /locus_tag="ACHKUB_001748"
     CDS             complement(33401..33967)
                     /gene="efp"
                     /locus_tag="ACHKUB_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6654776.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34009..35037
                     /gene="epmB"
                     /locus_tag="ACHKUB_001749"
     CDS             34009..35037
                     /gene="epmB"
                     /locus_tag="ACHKUB_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6654777.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8386 a         9650 c         8972 g         8244 t
ORIGIN      
        1 attaatgcgc tctcgctatt tgtcacatca atattttgtt caggtacagt ttgcagcgcc
       61 gtcgccagcg ccaacggatt ccatgtcata tattattcca tatagattta gtttaaatat
      121 taataaaatg aatatttgca gtacgtaatt atcttaccag ctatagacaa aaaaaaacca
      181 tccaaatctg gatggctttt cataattcag acgaactagc tgcgctgacg aaccgcttca
      241 aataagcaaa ttccggttgc aaccgaaacg ttcagggaag aaacacttcc tgccattggg
      301 atgctgatca actcatcgca atgttcacgg gtcaggcgac gcataccttc accttccgcg
      361 cccatcacca gcgccaggcg tccggtcatt ttgctctgat agagtgtatg atccgcctcg
      421 cctgccgtac cgacgatcca gatattctct tcctgcaaca tacgcatggt gcgcgcaagg
      481 ttagtcaccc gaatcagtgg aacgctttct gccgcgccgc aggctacttt tttcgccgta
      541 gcgttgagct gtgcggagcg atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc
      601 gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta caccgtcgag gatcagcagg
      661 aacggttgat cgagcgaagc gatcagatcc ggcagatcgt tttcctgata ctgacgtcct
      721 ggcttcacgc gggcgataat gccctgatgc acggcaccgt cgcttttctc gtcgagatat
      781 tggcggtttg ccaactggat aaccacgccc tgagactcaa gggcgtgaat cagcggtaac
      841 agacgtttat cttcacggcc ttttaaaata aagacttcct gaaaacgttc aggggcgcgc
      901 tccagcaggg cctgcactgc gtggatgccg taaatcattt cgctcattaa tgtactcgtt
      961 gtttacgttt tgcctgatgc actacgttta tccggcctac atgatctctg caatatattg
     1021 aatttgcgtg attatgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaagc
     1081 gcattttgtc agcaatctaa ccctcttctt ttaaagaggg tattgatcac tctgccactt
     1141 ttttcttcgc cgcacgcttc gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg
     1201 gctttttcgc ttttctcgcg tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc
     1261 ggaaggcgct gtctggctca aagtttacct ttttaccgac ctgacgacgc ttgccgcctt
     1321 ttttgcctgc atcgcctttt ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg
     1381 cgcgttcact ggagatcaga ctaaagtcga ttttgcgctc gtccatatta accgcttcga
     1441 ctcgaacttc cacgcgatcg cccaggcgat aagtctggcc gctggattcc cccatcaggc
     1501 gttgccctac ctggtcaaag cgatagtagt cattgtccag cgaagagaca tggaccagac
     1561 catcaatgaa caagtcgtcc agacggacga agaagccaaa gccagtgacg ctggaaatta
     1621 cgcctttaaa gacgttacct acctggtcga gcatgaagtc acacttcagc cagtcagcga
     1681 catcgcgcgt tgcttcgtcg gcacgacgtt ccgccatcga acagtgctga cccagttgca
     1741 acatctcttc catcgaataa tggtagccgc cggtttcagt ggtgttgccc tgatgcccct
     1801 gctctttcgc cagcagatac ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa
     1861 tcggcgaagt aaagtgcgca taggactgca atgccaggcc aaagtgacca cggttttctg
     1921 gatcgtaaat cgcctgtttc atcgagcgca gcagcatggt ttgcagcatt tctgcatcag
     1981 gacggtcggc aaccgactcc agcagttccg cgtagtcacg cggttccggc ttgttaccac
     2041 ccggcagctc cagccccagc tccgccagca ctgaacggaa agaggtaatc gcttcggtgc
     2101 tcggcttgtc gtgaatacgg aacagtgccg gttctttcgc tttctcaacg aaacgcgccg
     2161 ccgagatatt cgccagaatc atgcactctt caattaattt gtgcgcgtcg ttacgctggg
     2221 tctgttcgat acgttcaata cggcgttcag cgttgaaaat gaacttcgct tcttcgctct
     2281 caaatgagat cccaccgcgt tcttcacggg ctttatccag cactttatag aggttatgca
     2341 actcttcgag atgcttaacc agcggggcgt actgctcgcg cagatcctga tcgccctgca
     2401 gaatatgcca gactttggtg taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga
     2461 atttgtagcc cgtcaggcgg cctttcgacg aaaccgtcat ctcgcacacc atacacaggc
     2521 ggtctacctg cgggttgagc gaacacaggc cgttagagag cacttccggc agcatcggga
     2581 taacctgcga agggaagtac accgacgtgc cacggttacg cgcttctctg tccagcggcg
     2641 ttggcggacg cacatagtag ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc
     2701 cgcgtttttt ctcgcagtaa actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg
     2761 tgaccagcgg taaatcgcgc agatcaacac ggcccgcttt tgcttcttcc ggcacttctt
     2821 ctttcagccc ggcaacctgt tgctcaacag cctgcggcca gatgtacgga atttcatggg
     2881 tacgcagagc gatatcaacc gccatgccgg tgcccatatt gtcgcccagc acttcgacga
     2941 ttttacccac cgctttggtg cggcgagtcg gacgctgagt cagttcgact acgaccacaa
     3001 agcccatccg cgcgcccatg atctgatcgg gcgggattaa gatatcgaag ctcagacggc
     3061 tatcgtcagg aaccacaaag ccgacgcccg cttcggtaaa gtagcgacca acaatctggc
     3121 tggtttttgg caccagtacg cggacaatac gcgcttcacg acgaccttta cggtcagcac
     3181 ccagcggctg cgccagcacc tgatcgccat gaatgcaggt tttcatctgc tcgctggaga
     3241 gatacaaatc atctttacgc ccttcaaccc gcagaaagcc gtagccatca cggtggccaa
     3301 taacggtacc tttcaccagg tcgaggcgtt ccggcagcgc atagcactga cgacgagtga
     3361 agaccagttg accatcgcgc tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct
     3421 cttcgccttc aatgtgcagt tctaccgcca gctcatcacg gctggccggt ttttcacgtt
     3481 tggttaaatg ttcgaggata aattcccgac tagggatggg attcgcgtat ttttcagctt
     3541 cgcgttcctg gaaaggatct tgtgacatct cggttcctcc gttgtcatct ctgatgaaga
     3601 ttttcgtcac tccaccagca ataatttata aagcggttga ttctcttcaa ccaaatcggc
     3661 aagcgtgtag ttatccagtt ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt
     3721 caaccgacag gcaggtgtaa tgtggcaaaa ctcactgctg caattcacca gcgataaggg
     3781 ctccagctcg cgcaccacat caccaatacg tatcgcactc gccggtttac ccaggcgaat
     3841 gccgccattt tttccacgaa cagcagtcac gtagccggca cgactaagtt gattgattat
     3901 tttgaccata tgattacggg agacgccgta gacgtcagtc acttcagaaa tactggtcat
     3961 ccgcccttct ggcaatgacg ccatgtagat cagcgcacgt aatccgtaat cagtgaaact
     4021 cgttaactgc acatcaacct caaaagggaa atcgggaaaa aatacattta tattgatgat
     4081 aaaccagcca caagctgtgt cgctaattta tttcagttcg gggaaggaaa aaagcgagga
     4141 tttaacactg tgtcggatag cgtaatggct taaccgacac agcaatgtat cacactgtta
     4201 gcccggcagg caaaatatct gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag
     4261 aatcatggtt tcagtacgat ccggaccggt agagatgata tcgatcggca caccagtcag
     4321 ctcttcaata cgcttgatgt agttcagcgc cgcctgcggc agaccgctac gatctttcac
     4381 gccgaaggtg gattcagacc agcccggcat ggtttcgtaa atcggctcta cacctttcca
     4441 gtcgtcagct gccagcggag tggtagtcac ttcgcgacca tccggcatac ggtaagccac
     4501 gcagagttta acctctttca ggccatccag aacgtccagt ttagtcaggc agaagccaga
     4561 cagggagttc agctgtaccg cacgacgaac ggcaacggtg tccagccagc cggtacgacg
     4621 acgacgcccc gtagttgcgc cgaattcgtt accctgcttg cagaggaact cgccagtttc
     4681 atcaaacagt tcggtcggga acggacctgc acctacacga gtggagtaag ctttgaggat
     4741 acccagaacg taatcaacat aacgcgggcc caggccggaa ccggtcgcca cgccaccagc
     4801 agtggtgttg gaagaagtta cgtacggata agtaccgtgg tcgatatcca gcagcgtacc
     4861 ctgcgcacct tcaaacatga cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga
     4921 aacgtcaacc accatagaag tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt
     4981 ctggtaatca accgcttcag ctttgtagta gttaaccaac tggaagttgt gatattccat
     5041 cacttctttc agtttttcag cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc
     5101 gcgacgtgct actttatctt cataagcagg cccgatacca cgaccggtgg tgccgatcgc
     5161 tttcgcgcca cgcgctttct cacgcgcgtt atccagcgca acgtgataat caaggatcag
     5221 cggacatgct tcagacagca gcagacgctc acgaacgggg atgccacggt cttccagttc
     5281 tttcatctct ttcatcagcg cggccggaga cagcacaaca ccgttaccga tgatgctggt
     5341 tacattctcg cggagaatac ctgatggaat aagatggaga acggtttttt caccgttgat
     5401 tacgagagta tggcctgcgt tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc
     5461 agtcagaaga tcgacgatct tacctttacc ttcgtcaccc cattgggtgc ccagtacgac
     5521 gacgttgtta cccatttttc aaaatcaccg tttgcttaaa aatggattct accatcgctt
     5581 tttcagagtt acagcacttt ttgcactcaa aaatgacctc gatcagtctg ttttttgttc
     5641 agccaatcgt tttcctcaac atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc
     5701 caaaacgacg taaaatatta tcgggcaaat tggtcatcgc agagatcatc ttcttccatg
     5761 ccttcgggta aagcatcggc cctaaacctt ccagtaccaa aaccagggca agcgccagcc
     5821 agattgtcga attcattcat tatccttata gaaaaagaaa accaccgaca tccctgagga
     5881 tgcggtggct ttattgacct gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg
     5941 tcttcatgta gcggaagaaa tcgctatccg ggctcatgac catcacgtcc tgattgccag
     6001 agaagctgtt ctcataagca cgcaggctac ggatgaatgc gtagaagtcc ggatctttac
     6061 tgaatgcatc agcaaacagt ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc
     6121 cctgacgctc agcttctgcc agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt
     6181 tttccgcttc ttcctgacct tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc
     6241 gcatacggtt gtagatcgct tcagacactt cggtcggcag gttgatctgc ttgatacgca
     6301 catcgacaac ttcaataccc agcgccgcca tactgttcgg gttgatgacc ggaactttgc
     6361 ccttcgtctc agccgttacg cgctctgccg cttcggcaat ggcgttatcc gctgccgggg
     6421 tagtaacttc atcttctgta cccgcagaac cggagttcag cgcgtcacgt acttcgaggg
     6481 tcagacgacc acgggaatcg gtgacgatat ctttcacgtc caggcgacca atttcagaac
     6541 gcagacggtc agagaactta cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac
     6601 ccgttgccag gtagtaacgg ctgaaatcgc tgatgcgcca tttgatgtaa gagtcgacga
     6661 tcaggtcttt cttctctttg gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac
     6721 gtgcgtcgag cattttcacc gtttcaatga acggtatctt gaaatgcaga ccaggctcat
     6781 aaaccaaagg tttgttgtca tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc
     6841 gctcaccttc tttgacgaca aagacagaca tgtaaagcac taccagcacg atgatgataa
     6901 tcgcgataac tgacttacgc atcgttattc cccctgacgc tggtagtcgt tacgctgcgc
     6961 gttggcgcgg cgttggtcca taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc
     7021 actggttgtg gaggaagagg ctggcggcag acgcagcaga ttgctggcac cgttatcgct
     7081 cttcgccgca ggggcgttac cacctttcag catctggtct aacggcagaa ccatcaggtt
     7141 gccaccttta tcgttaacca gcactttgcg ggtgttaccc aacacttttt ccatcgtctc
     7201 gatatacaga cgctcgcgag taatttccgg cgcggcttta tattccggca gaagtttagc
     7261 aaagcgcgcc acttcaccct gagcttccag gatggtctgg gccttgtacg cacgcgcctc
     7321 ttcgaggata cgttgcgcct gaccgttcgc acgcggctga acttcgttgg tatacgcttc
     7381 tgcttcacga atgtattgct gttcgttttc acgcgcggca atcgcatcgt caaacgccgc
     7441 ttttacttct tccggcggac gagcagcctg gaagttgacg tccagcagcg tgatacccat
     7501 gtcatacgga cgaatcgtct cttccagttc gcgctgagta tcgctacgaa tcacggtacg
     7561 accttccgtc agaatgcggt ccatggtgta tttaccgata actccacgca gggcgctgtc
     7621 ggtagcctga cgcaggctgt catccgggct ggtcacgcta tacagatatt tttccggatt
     7681 ggtgacgcgg tactgcacgt tcatctcaac gcgcactacg ttctcgtccg acgtcagcat
     7741 cacaccagaa gcggccagtt cacgcacggc ttccacgttc accggtttga cttcgtcgat
     7801 aaacgtcggt ttccagttca gacccggctc aaccagatgg ctgaatttac caaagcgtgt
     7861 taccacgccg cgttcggctt ctttaatggt atagaaacca ctggccgccc agataatgac
     7921 aatcgctgcc gctgcgatgg taacgacacg accgccaagc tgcgggcgcg ggccttgcga
     7981 tgaactgcca ccgccagatc cggtgccttt accgccgccc agaccaccga gctttttgct
     8041 cagtttgcgg aagatatcat ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc
     8101 atttccctca gagttgccgc caggtttgct gcttccccac gggtcgcggt cttgtccgtt
     8161 attaccgggc tgattccacg ccatgtttgt gctccatatt tgttatgcgg tgatccctgt
     8221 tgtctttcga cgccagagga tatgactcca cgcttcagac gctacgccgt tagatcaggt
     8281 aatcgatcaa cgccggttct tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa
     8341 cttgcagact tacgctgccg tcctcctcca tccactcttt ttctattgcc tgaagctgat
     8401 aaaaacgact tctcagacgc ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct
     8461 cgccggaaag ccgctccgtc aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg
     8521 cggaaagcca gacacggatc ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat
     8581 cttccagcat atcgatcttg ttcatcacca gcagggttgg gatctcgtga gcgtcgatct
     8641 cttcaagaac cgtattcacc gcttcgatgt tttcttgtac acgcacatcc gccgcatcaa
     8701 tgacgtgcag cagtaatgtg gcttgccgcg tctcttgtaa cgtggcttta aatgccgcca
     8761 ccagatcgtg cggcaggtgg cgaataaacc ctacggtatc tgcaagtacg gtttcaccga
     8821 catctgcaac gtcaatacgc cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg
     8881 cgtagacccg cgcttcggtg atgcgattga aaagggtaga tttaccggcg ttggtatatc
     8941 ccaccagcga aacagtagga acgtcggctt tgatacgcga ttgccgcccc tgctcacgct
     9001 gcttttcaac tctttccagg cgtgactgta tctgcacgat gcgattacgc aacaaacgac
     9061 ggtcggtttc gagctgggtt tcacccggac cacgcaaacc tatcccgcct ttctgtcttt
     9121 caaggtgggt ccagccacgc accaggcgcg tagccagatg gcgcagctgc gccagctcaa
     9181 cctgcaactt accctcatgg gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg
     9241 tgcggtcgat aacacgacac tcgcacaaac gctccaggtt acgctcttgc gccgggctca
     9301 gggcatggtc aaaaagaacg accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa
     9361 ctgctttacc ttcacctaca aaatactttg ggtgcggcgc tttacggcta ccggtaatca
     9421 cctgcaatgc ttcgacaccg gcggaagaga ccagagattc aaactcctgg aggtcttcca
     9481 tatctttgtc ttgcgtaaaa tagatgtgta ccagtaccgc ctgctcacca gcatcataac
     9541 ggtcaaacaa gcgtataacc ctctaaatag atcagcgggg aacgcaggat cgctggctcc
     9601 ccgtgtaaaa aaacagcccg aaaccttatt cggtttcttc gctgtcctgt tgcgcggaag
     9661 tattctgcgc gctgctacca tgatggtagt tactgctggt accgccaccg gcgttgttac
     9721 tgtgatgaga aaccggacga gacgggacaa cagtagaaat cgcgtgcttg taaaccatct
     9781 ggctgaccgt gtttttcaac aggatcacga actgatcaaa agactcgatt tgcccttgca
     9841 gcttaatacc attcaccaaa taaatagaaa ctggaacacg ttcccgacgc agtgcgttca
     9901 ggaacggatc ttgtaaagat tgccccttag ccattctctc ttttccttat atgcttattt
     9961 gtactttgaa cctttcgatt ctgaaaaaat tgcgcacgat acgtctcaat tgtacacatt
    10021 cagcctgcga tagcaccaac aacctgtaat acttcgtcac gcgcctgttc tggtttttca
    10081 ctgtcaagcc agtgaacccc ttcccaacca cgcagccagg ttatctgccg cttcgccaac
    10141 tgtctcgtgg cgcaaacacc tcgataaacc atttcatcgt atgagatttc gccttcaagg
    10201 taagaccaca tctggcgata acccacgcaa cgaatggaag gcaagtccgt atgcaaatct
    10261 cctcgggcaa aaagcgcccg gacttctgct tcaaaacctg aagccaacat ctgatgaaaa
    10321 cgctgctcaa tgcgttgatg gagcagttca cggctcgccg gggcgatggc gaactgatgc
    10381 acctgatacg gtagagcgtc tcctgacgtt tgcgtcagtt ccgttaaagt tttacccgaa
    10441 atgaaaaaaa cttccagtgc ccgggaaagc ctttgtggat catttggatg aatccttgct
    10501 gccgcaaccg gatctacctc ctgaagttga cgatgcaatg actcccaacc ttgctctgcc
    10561 gcctgttgct caattctggc ccgcacttcc gggtctgccg acggtagcgg cgacaaccct
    10621 tccagcaatg ccttgaaata caacatcgta ccgcccacta acagtgggat ccgccccgcc
    10681 gcggtgatat cggccatttc cgccagcgca tcgcggcgaa aatcagcagc cgagtaagcc
    10741 tgcgacggat cgcgaatatc cagcaatcgg tgcggcgcgg cgagtaactc ttcagcgttc
    10801 ggcttcgccg tcccgatatc catccctttg taaataaggg cagaatcaac gcttatcaac
    10861 tctactggta aaattttacg cagctcaatg gctaacgccg ttttaccgga ggccgtcggc
    10921 cccatcaaaa aaatcgcctt aggcaggctc gccttactga tatcactcat ctttcagggc
    10981 ttttatcgcc ggatgtaaat caacagattg taacagacca cccggcggcg ttttcacaag
    11041 ttgcggacat aaccgctcca cgtccgccag cagggttatg gcctgtgcca ttgaccactg
    11101 cgcatgttcg ctcatcagat ttcgtgcaat ccactgcgca atattgccag gttcgaatac
    11161 ggactgcttc gccaggtagc ctatcagttc aggaatcaag atttgtaaat tttgttggcg
    11221 taagggtaaa ggcacggccc tgatggtcac atgctgtgca tctgactgga aatcaatacc
    11281 caattccgcc agggcagact gcgctttttc taatgccgat ttttcttcgg cagaaacttt
    11341 tagccgcaac ggaatcagca gcggctgggc gcaaacgggc gcttcacccg gcgtcaattg
    11401 tgcctgacgt agccaacgtt ctgccactgg caaggataaa agtgaaatgt tgccatcgcg
    11461 ctccagcaac gcacagtcgg aatggacgat agtcagtacc cgaccaaaac tctgactgtt
    11521 cgccgcaagt gcaggttcct gcggttccgg cgcttttggt ttttgcatcg gcgcgggcgt
    11581 ttgcaaaagc tggcgataca cttcaccttg ctgtttctgg tagcctggct gcgcattcgg
    11641 ccagggggca gccggacgac tgcctgatgc tggcgcagga gtgtagcgcg gagctaccgg
    11701 ctcacgagct gccggttctg caaagtgatt gcgccccgcc gccacgcggt tttccggaat
    11761 ggaacgcggt gcaggttggg gttcatcgtc cagcggtagc ggcgtttcca gttgctgttg
    11821 tagcacgctc agcacgccct gatagataaa atcatgcacc agacgcgact gatggaaacg
    11881 cacttcgtgt ttggcggggt gcacgttgac gtccacctga tgcggatcga tctccagata
    11941 cagcacaaat gccggttgct gatcggcccc cagtttgtct tcgcaggcct ggcggatcgc
    12001 gtgattgatc aggcgatcgc gcatcatgcg accgttcacg tagcaatact gaatttctgc
    12061 cagtgcgggc gtggtgtgat ttggatcggc cacccagccg cgtagcgtga gatcgccgtg
    12121 ttgccattca atcgccagcg cttgttcaag aaaagcggtg ccgcaaatcg cgcctaagcg
    12181 ccgttctttt tgcccgcctt ccggcactgc gcggtactga cgcacaattt taccgttatg
    12241 cgacaggttg atcgtgacgt cgaaacgcgc cagcgcaatg cggcggatga tctcatcaat
    12301 gtggttaaat tcggttttct cggtgcgcag gaatttgcgc cgcgccgggg tgttgtagaa
    12361 cagatccagc acctccagcg tcgtccccac aggatgcgcc gccggtttta ccgtcacgtt
    12421 catatcgcgc ccttcggcat aggcctgcca ggcttcctgc tgttctgcgg tgcgtgaagt
    12481 gagcgtcagg cgggaaaccg aactgatact cgccagcgcc tcaccgcgaa agcccaggct
    12541 gataatggct tcgagatcgt cctgagaggc gattttactg gtggcatgac gagccagcgc
    12601 cagcgccagc tcatcttttt tgataccgca gccgttatca cgaatgcgga taagtttcgc
    12661 cccaccgcgt tcgatatcaa tatcgatacg cgtcgcacct gcatcgaggc tgttttccac
    12721 cagttctttg actaccgacg caggtcgctc gaccacctca cctgcggcaa tctggttcgc
    12781 cagttgtggc ggtaagacct gaattggcat caatcgtcct tagtttggca gcgtgcgatc
    12841 tggcgtcgtc accgtactgg cagtttgtgc cgttgccccc tgcggcgcag attgcatcgg
    12901 atgcgcaagg aaataattgc gcaggccttt gtaaatggct tctgccagct gttgttggta
    12961 atcgtcgctc gccagcaaac gttcttcgct gttgttgctg ataaaaccgg tttcgaccag
    13021 tactgacggg atatccggcg aacgcagaac gccgagactc gcgtgttctg gtcggcgttt
    13081 atgtatttcg ccaatgcgtt gcaactgact gatcatactg gtcgctacat cataccctac
    13141 ccgttgggaa tgaccgaact gtaaatccag cacagcctgg cttaaatagg ggtcagactg
    13201 actgttcgcc agcacatcac ccgccccacc cagcagctcc gactgtttct cgtgctgctc
    13261 cagccagctg gccatttcgc tgttggcgcg acggttagag agcacccata cggaagcgcc
    13321 agtcgcactg cggttcggtg cggcatcagc gtgaatcgac acgaggaaat tggcgttttg
    13381 cttacgtgcc acatcactgc gccccatcac cgagataaag taatccccgt cacgggttaa
    13441 aacgccctta aacatcggat cgtcattgag caaagtacgc aatttacgcg cgatggcgat
    13501 ggtgacattt ttctcccgcg taccaccggg gccgatagcg ccagggtcct gaccgccgtg
    13561 tccggcatca atagcgatga taattttatc gccagtgtta gccgtcgcgc gcgctgccgg
    13621 acgcgttacc gtattactgc tgataacacc cgtagtgcgg ttactttccg ttttaaacgg
    13681 attgcgcgcc ggttcgctga cgcgcggtgc gacaaccgca ggcgtttcaa cgcgtttcgc
    13741 aaccacgggc ggcggaggag gaggtggcgg cgcatcggcg ttaatcgtaa agacgacagt
    13801 gtaattgctg ccattctgcc gcttcaccgc ttcggtttta ccgttttcgg taagatcgac
    13861 caccagccgt agcgtttgtg catctttagg cgttccagag cgaatcgcct tcaccagatt
    13921 attgccgctg aacaacaacg gcagtccctg aatcacgccc gtttgtttga tatcgagcgc
    13981 cacggtgcgt ttgctttgat ggctaaacgc ataatcagga tcgccaataa aactcaacgt
    14041 tatccgcgcc tgttgattac cgttagaaac ctgaatatca gagagcgtcg cggcacccac
    14101 cggcgtgcac agcagcagca gcgtcgctac caaccaattt ctgatgcgat acatcatccc
    14161 gccacctttc aaaggttaac cggctaaacg cgccagcaac aattcacccg cagaggaaac
    14221 cgcactcacg cgcgcctcac ggccttgtgc ctgataatca atgtgtattt cgacatccgg
    14281 gtcaggaaga acaccagtac cttgttgtgg ccactccacc aggcagatgg catcgttggc
    14341 aaaataatcg cgaatcccca taaactccag ctcctcggga tcggcaaggc ggtacaaatc
    14401 aaagtgataa accattaaat tgtcgagcgt atagggttcg accagcgtat aagtggggct
    14461 tttgacatta ccctgatgac ccagagcttg taaaaagccc cggctaaagg tggttttacc
    14521 tgcgcctaaa tcgccataca gatagattac ggttgcgcca tcgcaggctt tcgctacccg
    14581 ctcgcccagg tctaatgttg cctgctcatc agggagcgga attactcgat tcatcatggt
    14641 ttttatcagt cacttccggg ttaacaatac gctgtagcgt ggaaaagaga tcggttgcca
    14701 gcatcccgcg cgttccaaaa cgcgccgcca gtacgtcagc tgcagcaccg tgcgcgacac
    14761 agcccgcaca ggcggcatca tacggcgaca gtttttgccc aagcaatgcg ccaataatac
    14821 cagagagcac atcgcccatg ccgccgctcg ccatgcctgc atttccggca tcaataatgc
    14881 ctaaagcgtc aggatgggcg gcgaccacgg ttccggcacc tttcagcacc gctacgccgc
    14941 cataacgttg taccagacgt ttggcgcaat gtaagcggtc actttcaatt tcagcgacgg
    15001 aacagcctaa caaccgtgcg gcctcgccag gatgcggcgt gatcacgcga ttgtgacgct
    15061 tatcgggatt gattgccagc aggttcaatg catcggcatc ccacaacatc ggtttgcgaa
    15121 aattctcaac tttttgcagt gcttttttcc cccactcttg ctggcccaga ccgggaccaa
    15181 tcaccaccac atcggcccat tccaggcttt cggtaagaga gtccatcgtc agttcatgca
    15241 ccatcaattc cggtcgtgca gtcagcagcg gcgcaatgtt ttcactgcgg gtcagtactc
    15301 ggactaaacc agcaccagca cgtagcgccg cttcccccgt catacgaata gcccccgccg
    15361 tgccgtgatc gccaccgata attaccagcc gcccgtgatc gcctttatgc gaagtcgggc
    15421 gacgcggttt tagccagtga gaaagttgtt ctgccgaaaa ccgctgaatt ttcgtctcct
    15481 gacctgccag ccaactatcc agccccagtg agtcaaaatg cagttgtccg gtaacatccc
    15541 gcgcttttcc agtgagcaag cctggtttca gcgcaataaa agtgatggtg tgatcggcgt
    15601 tgatcactgc gcctggcgta gcgccggttt cagccagcag gccggaaggg atatcaaccg
    15661 ccgcaatcgg cgcaggatgg gaattagcgt ggtcgattaa ctggctaatg gattcgcggg
    15721 gcgcttgctg caagccggta ccgagcagcg catcaacaat cagatctacc gattcgggcc
    15781 agacaatatt cgaagcatgg atctcgcctc ccgcgtttaa ccatgcttcg cgtgccagcg
    15841 cggcctcttc cggcaacggt ttgtcgctct cctgggccag cagcgtgacc tcaatgccga
    15901 ccgctgtggc cagccgcgcg accacgtagc catcgccgcc gttattacca tgaccgcaca
    15961 gcaccagcca atggcgggcg tcaggatacg ccgaacgaca cacctggaat gcggcttcgc
    16021 cagcgcgaag catcagctca tagagtgtaa gccccagcgc atctgccgcc tcacgttctc
    16081 cgcggcggat atcgtcggcg tgccagacgg tgtgtggtat acttacgggg tttttcttca
    16141 ttgtatggtc cgtcatgtca gagcccctcg atctcaatca gttagcgcaa aaaattaaac
    16201 agtgggggct ggaactgggc tttcagcagg taggtattac cgataccgat ctcagcgagt
    16261 ccgagcccaa actgcaagca tggctggaca aacaatacca cggcgaaatg gactggatgg
    16321 cacgtcacgg tatgctgcgc gctcgccccc atgaattatt gcccggtacg ctgcgcgtga
    16381 tcagcgtgcg gatgaattac cttcctgcta acgccgcatt tgccagcacg ctgaaaaacc
    16441 ccaaactcgg ctatgttagc cgttatgcgc tgggccgtga ctatcacaaa cttctgcgca
    16501 accgactcaa aaagctgggc gagatgattc agcaacattg tgtttcgctg aattttagac
    16561 cgtttgtcga ttctgcgcct attctcgagc gcccgttagc tgaaaaagct gggctcggct
    16621 ggacaggtaa gcactcactt atcctcaatc gcgaggccgg ttctttcttc tttttaggcg
    16681 aattgctggt cgatattccg ctgcccgtgg atcaaccagt cgaggaagga tgcggtaaat
    16741 gcgtggcctg tatgacgatt tgcccgaccg gtgccatcgt cgagccatat accgtcgatg
    16801 ctcgccgctg tatctcttat ctcaccatcg aacttgaagg ggcgatcccg gaagagttgc
    16861 gaccgttaat gggaaaccgt atttacggtt gcgatgactg ccagcttatc tgcccgtgga
    16921 atcgctattc acaactcacc acagaagagg atttcagccc gcgtaagccg ctacacgcac
    16981 cggaactcat tgagttattc gcctggagcg aagagaagtt tttaaaagtc acggaaggat
    17041 cggcgattcg tcgtattggt cacctgcgtt ggctgcgtaa tatcgccgta gcattaggca
    17101 atgccccttg ggatgaaacg attttgacag cgctggaaag tcgtaaaggt gagcacccac
    17161 ttctcgatga gcacatagcg tgggcgattg cgcagcaaat agagagacga aatgcgtgca
    17221 tagtcgaagt gcaattgccg aaaaaacagc gtctggttcg ggtgattgaa aaagggttac
    17281 cgcgtgacgc ctgattcatc cacagcttgt gaataaaaat aaaaacgcat tgcaattcaa
    17341 gacgaataaa atcgtcaagt gatcgactcg acaaaacgga atcttaattt ttaacttatc
    17401 tatcaatagg ttaaaaaaat actattcacc gtgcgaagtt tctttgaaat taagattaca
    17461 gcctgaggct gtggataact ctgtttacaa gagtttgtca gagacaacga aaaccagcaa
    17521 taacgcatcg catcgctgtg gatattttat tgagagaaga atttggagcg ggaaacgaga
    17581 ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc tattcccgct
    17641 tgggtggtct gtgccttaca gcactttcaa attttggagc gggaaacgag actcgaactc
    17701 gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc
    17761 tgtgctttgc agcacttttc aaattttgga gcgggaaacg agactcgaac tcgcgacccc
    17821 gaccttggca aggtcgtgct ctaccaactg agctattccc gcgtactact taattttgct
    17881 ttcgtaattt tcgcatttct gcgtcgttac gggaggcgca ttatacgaga aatctttccc
    17941 tctgcaaccc cccaaaaaag ttttttttgc gtttttatct caagtgctga aattatcagc
    18001 aagacgttca atttagcgtc aaaatcacgc cgccgcaagt ggattccggc gcttacgtga
    18061 ccaggaaaaa ttttacagct tgataaaatg ctcgcggtag taagccagtt ccgccaccga
    18121 ttcacggata tcatccatcg cctgatgcgt cccctgcttg gtaaaaccat ccagaatttc
    18181 cggcttccag cggcgcgcca gctctttcag ggtgctgaca tcgagataac ggtagtggaa
    18241 gtaggcttcc agctccggca tgtatttaaa caggaaacga cggtcctgtc cgatgctgtt
    18301 accgcaaatc ggcgattttc ccgcaggcac ccactgtttt aagaattcga gcgttgccag
    18361 ttcagcttcc cgatcgccca tcgtgctcgc tttcacgcgc tctaccagcc cgctggcggt
    18421 atgggtgcgc acgttccagt catccatcag cgccagctgt tcatcggact gatgtactgc
    18481 aatggtcggc ccttctgcca gaatattcag gttggcatcg gtcaccagcg tggcaatctc
    18541 aataatgcga tcgcgctcgg gatccagacc ggtcatctca agatcgatcc aaatcaggtt
    18601 gttttcattg gcactcatgc tattttccac ccattcacag tgactatatt gatccacaat
    18661 tgcgtgtatc atagatgttt tgcccatcag gggcgaccag gagtctgtac gattgagtaa
    18721 aaataaactc tccaaaggcc agcagcgccg cgtgaacgcc aatcaccagc gtcgtcttaa
    18781 aacgtctaag gagaagcccg actacgacga caatctgttt ggcgagcctg atgaaggtat
    18841 cgtcatcagc cgctttggta tgcacgctga tgtggaatcc gccgatggcg acgttcaccg
    18901 ctgcaatatt cgccgtacca tccgttcgct ggtgaccggc gaccgcgtag tctggcgtcc
    18961 gggtaaaccg gcggcggaag gcgtaaatgt caaagggatc gtggaagcgg tgcatgagcg
    19021 cacctcagtg ttgacgcgtc cggatttcta cgacggcgtg aaacctattg ccgccaacat
    19081 cgaccagatt gtcattgtct ccgccatttt gccggagctg tcgctcaata ttatcgaccg
    19141 ttacctggtg gcctgcgaaa ccttgcagat tgagccgatt attgtgctca ataagataga
    19201 cctgctggac gacgaaggca tggcgttcgt caacgagcag atggatatct accgcaacat
    19261 tggttatcgc gtattgatgg tttccagcca tactcaggat gggctaaaac cgctggaaga
    19321 ggcgttgacc gggcgcatca gcatttttgc cggccagtct ggcgtcggca aatccagcct
    19381 gctgaacgcg ttgctggggc tgcaaaaaga gatcctgacc aacgatgtct ctgacaactc
    19441 gggtctcggt cagcacacca cgaccgccgc acgactgtat cacttcccgc acggcggtga
    19501 tgtgattgat tcccctggtg tgcgtgagtt cggcctctgg cacctggagc cggaacaaat
    19561 cactcagggc tttgtcgaat tccatgacta tttaggtctg tgtaaatatc gcgattgcaa
    19621 acacgatacc gatccgggct gcgctatccg ggaagcggtt gaggaaggaa aaatcgcgga
    19681 aacccgtttc gaaaactatc accgtattct ggaaagcatg gcgcaggtaa aaacgcgtaa
    19741 aaacttttct gatacggatg actgacaatt aagctaaccg tcgttagaat cgtccccttt
    19801 ttttcaggat ccggcatgta ggccggatca ggaacgacaa aacaatggcc tggaggctac
    19861 cttgttaaat tcatttaaac tttcgctaca gtacattctg ccgaaactat ggcttactcg
    19921 cctggcgggt tggggcgcaa gcaagcgggc aggatggctg acaaaactgg ttatcgatct
    19981 gttcgttaaa tactacaagg tcgacatgaa agaggcgcaa aagccggaca ccgccagcta
    20041 ccgcaccttt aacgaattct ttgtccgtcc gctgcgtgac gaagtacgcc caatcgatac
    20101 cgatccgaat gtcctggtca tgcctgccga tggcgttatc agccagttgg gtaaaatcga
    20161 agaagataaa atcctgcaag ccaaaggcca caactacagc ctcgaagccc tgctggcagg
    20221 caactatctg atggcggacc tgttccgcaa cggtacgttt gtgaccactt acctctcccc
    20281 gcgtgactac catcgcgtac acatgccgtg caacggtatt ctgcgtgaga tgatctacgt
    20341 accgggcgat ctcttctccg ttaaccatct cacggctcag aacgtgccga atctgtttgc
    20401 ccgtaacgaa cgcgtgattt gccttttcga taccgaattt ggcccgatgg cgcagattct
    20461 ggtcggggcg acgattgtag gcagcattga gacggtctgg gcgggcacca ttacgccgcc
    20521 gcgcgaaggt atcatcaagc gctggacctg gcctgccggg gaaaaagacg gttctgtggc
    20581 actgctgaaa ggccaggaaa tggggcgctt taaactcggc tctaccgtta tcaacctgtt
    20641 tgcaccaggt aaagtgaacc tggttgagca actggaaagc ctgtctgtta cgaaaattgg
    20701 tcagccgctg gcagtatcta ccgaaacctt tgttacgcca gacgctgaac cggccccgct
    20761 tcctgctgaa gagatcgaag cagaacacga cgccagccca ttggttgacg ataaaaaaga
    20821 ccaggtctaa tccatcaaag gaaacgctga cgtgcgcctg attatcactt ttctgatggc
    20881 ctggtgcctc agttgggggg cgtacgccgc gacggccccc gatagcaaac aaatctcaca
    20941 agaactggag caggcaaaag cggcgaaacc cgcgcagccg gaagtcgtag aggcgctcca
    21001 gtccgcctta aatgcgcttg aggaacgaaa aggttccctt gagcgcatca agcaatatca
    21061 agaagtcatt gataattacc cgaaactttc cgctactctg cgcgcacaat taaacaatat
    21121 gcgtgacgaa ccgcgcagcg tgtcgccggg gatgtctacc gacgcgctga atcaggaaat
    21181 tctccaggtc agcagccagt tgctggataa aagccgtcag gcccagcaag agcaggagcg
    21241 cgcccgcgag attgccgatt cgctgaatca actgccgcaa cagcaaaccg acgcccgccg
    21301 tcagttaaat gagatcgagc gccgcctggg aacgcttact ggcaataccc cgctcaatca
    21361 ggcacaaaat ttcgcgttgc agtctgactc cgcacgcctg aaagcgctcg ttgatgaact
    21421 ggaactggcg cagctgtctg ccaataaccg tcaggaatta gcgcgcttac gctcagagct
    21481 ggcggaaaaa gagagccagc aactggatgc gtatttgcag gccttacgta atcagttgaa
    21541 cagccaacgt cagcttgagg cggagcgggc gctggaaagt accgaattgc tggcagaaaa
    21601 cagcgccgat ttgccgaaag acatcgtcgc gcaattcaaa attaaccgcg aactatcggc
    21661 ggcactgaat caacaggcgc agcggatgga tctcgttgcc tcgcaacagc gccaggccac
    21721 cagccagacg ttacaggttc ggcaggcgct gaatacgctg cgtgaacagt cgcaatggct
    21781 gggatcgtcc aacctgctcg gcgaagcgtt gcgggcgcag gtggcacggc tgccggaaat
    21841 gccgaaacca caacagcttg ataccgaaat ggcgcagttg cgtgtgcaac ggttacgtta
    21901 tgaggatctg cttaataaac agccgctgct acggcaaatt catcaggccg acggtcagcc
    21961 gctgaccgcc gagcaaaacc gtattctgga agcacaacta cgcactcagc gtgagttgct
    22021 gaactcattg ttgcagggtg gcgacacgct actattggaa ctgaccaagc tgaaagtctc
    22081 caacgggcaa ctggaggatg cgctgaaaga ggtgaacgaa gcaacgcacc gctatctgtt
    22141 ctggacctct gacgtgcgcc cgatgaccat cgcctggccg ctggaaatcg cccaggatct
    22201 gcgtcgtctc atttcgctgg acaccttcag tcagttgggc aaagccagtg tgatgatgct
    22261 gaccagcaaa gagacaattt tgccgctgtt tggcgcgttg attctggtcg gttgcagtat
    22321 ttactcgcgc cgctatttca cccgttttct tgaacgttcg gcggcgaaag tcggcaaagt
    22381 gactcaggat cacttctggc tgacgttacg cactcttttc tggtcgattc tcgtcgcgtc
    22441 accgttgccg gtgctgtgga tgacgctggg ttacggcttg cgcgaggcgt ggccttatcc
    22501 gctggcggtc gcgattggcg atggtgtaac ggccaccgtg ccgctgctgt gggtagtgat
    22561 gatttgcgcc acctttgccc gcccgaacgg cttgtttatc gctcattttg gctggccgcg
    22621 cgaacgtgtt tcccgtggga tgcgctacta cctgatgagc atcgggctta ttgtgccgct
    22681 gattatggcg ctgatgatgt tcgataacct cgacgaccgt gaattctccg gttcgctggg
    22741 acggctttgc tttatcctca tttgcggtgc gctggcggtg gtcaccctca gcctgaaaaa
    22801 ggccgggatc ccactgtatc tcaacaaaga gggcagcggc gacaacatta ccaaccatat
    22861 gctgtggaac atgatgattg gcgcgccact ggttgccatt ctggcgtcgg cggtgggtta
    22921 tctggcaacg gcacaggcgc tgttagcgag gcttgaaacc tcggttgcca tctggttcct
    22981 gctactggtg gtttatcacg ttatccgccg ctggatgctg atccaacgcc gcaggctggc
    23041 gtttgaccgg gcgaagcatc gccgggcaga gatgttagcg caacgtgcgc gtggcgaaga
    23101 ggaagcacat catcacagta gcccggaagg ggcaattgaa gtcgatgaaa gcgaagtcga
    23161 tctcgatgcc atcagtgcgc aatccttgcg gctggtgcgc tcaattttga tgttgatcgc
    23221 cctgctttcg gtcattgtgc tgtggtcaga aatccattcc gctttcggct tcctcgaaaa
    23281 tatttcgctg tgggatgtca cctccacggt acagggcgta gaaagtctgg agccaattac
    23341 cctcggtgcg gtgctgattg ccattctggt gtttatcatc accacgcagc tggtgcgcaa
    23401 cctgcccgcg ctgctggaac tggcgatttt gcagcacctg gatttaacgc cgggtacggg
    23461 ttacgccatc accaccatca ccaaatatct gctgatgctg attggcgggc tggtcggctt
    23521 ctcaatgatt ggtattgagt ggtcgaaatt gcagtggctg gttgccgcgc tcggtgttgg
    23581 tctcggtttt ggtttacagg aaattttcgc caactttatc tctggcctga tcatcctgtt
    23641 cgaaaaaccg attcgcattg gcgatacggt aacgatccgc gatcttactg gtagcgtgac
    23701 gaaaattaat acccgcgcaa ccaccatcag cgactgggac cgtaaagaga taatcgtgcc
    23761 gaacaaggcg tttattaccg agcagtttat caactggtcg ctctctgact cggtcacgcg
    23821 cgtggtgttg acgattccgg cccctgccga tgccaacagt gaagaagtga cggaaatcct
    23881 gctcaccgca gcgcgtcgct gctcgctggt gatcgacaac ccggcaccgg aagtcttcct
    23941 ggtggatctg caacaaggga ttcagatttt cgagctgcgt atttacgccg ctgagatggg
    24001 tcaccgtatg ccgctacgcc atgagatcca ccagctgatt ctggcaggct tccatgccca
    24061 cggtatcgat atgccattcc cgcccttcca gatgcgtctg gaaagcctga atggtaaaca
    24121 aacggggaga acattaactt ctgcgggcaa aggtcgtcag gcgggaagtt tgtaatgcat
    24181 gaagctgcca aatgatgtca acgtggctca tttagcagct taacatcatc ttcagttatc
    24241 accggccctg gagagtatca tccatacgca ttacttcctg acaactcatc gccggagaga
    24301 cgatgagttg caggatttca tatgatcagt cgagagctaa ctcaggttta ttcgccatgc
    24361 gactgcaata gttctggtaa atcgccatcg ctaacagcga gaagaagaca gggccgccaa
    24421 tcatccacaa tgtgctgtcc cagtctccag cttcaaccac aggttgaatg atggtgaaga
    24481 cgttcgcaaa tgtcaccacc agtacgacaa ccactgttgc aatcattgcc gacatatgcg
    24541 ttttgaaaat cacaaatggt ctgtcgagat cctgacgtgc tttaaaaaat gggaaagcca
    24601 gcgcgaggaa caggtaagga agcgtcatag acacgttcgc catcagcgtc agcttgttaa
    24661 agaacgccga tgcggtaccg ccaccaaacg aaaccagcag gatgaagaca gtaaccaacc
    24721 cgcactgcat ccacatggcg atagacggca tccccatcgc attcaggcgc gtcatcggtt
    24781 ccggccacaa cgctttcggc gtcccctgga tgatggcttt cagcggtgaa tagcaaagcg
    24841 taaagaacgc accggtataa gcgaggaaca tcgaaagtcc ggtaatacgc gcaaaccata
    24901 cgcccagcga caatgacgct tcaggtgaca aatgcagtgc gttacccagc gtcaccccga
    24961 ggctcttcat cagcacatag gtaatattgc caaggttaac ggaaccatta cttaatacct
    25021 gctgccagtt tgtgctgacg ccccataaaa atattgccag cgaataaccg attgaaataa
    25081 caatagcggc aaaaacaata cctttggcaa agttcttttc tggattttcc gttttatcga
    25141 ccagaccacc aaccgcttca attccgccat aggcaaaaat agcaaatacc acaaacgata
    25201 gcattgccag accggactga taacccgggt tcggtgatgc aaggaaatta atatcctgcg
    25261 cgaaatgccc accatttaat aacaaaatag taatgcttac taacagcaat actaaattca
    25321 gacacattac tgcaataccg cccaccgcag taatgcgggc aattttatta atcccctttg
    25381 aagcaacgac ggtgaccaga atcatccatg ctactgccag cagaccaacc acctgcgtag
    25441 gctccagtcc ggcaatacgc cagtgcaggg tcatgtcgct accatagagg aatgttgaga
    25501 acggtaccca aactttcgcg gaggtactca ccatccagat gatataagag gaaaaccaca
    25561 taaacgtacc aatgaaggca aaacgtggtc cgacactatt attcatccag gaatagatac
    25621 caccttcttc tttgcgataa gcagctccca tttcagccat cattaaggcg aatggaataa
    25681 agaataacaa tgcagaaaat atataaaagg gaatcgcact ataacccatt aagtaataag
    25741 ccgatgggct attggcaaat ccaaatacgg aagtaaagat catcaatatg agtcctatca
    25801 gactcatctt ttttatcgtg tgaggcatga aaccatcctt catttcagcg cgtgtataac
    25861 acgtgcccac tgaactgcac tgcgcaacat tgagcaggca gcgtaaaaac caatacagaa
    25921 ttgatacctg gatgatatca aatatccagt cttaaattgt ggcctttaac ggaaaaatga
    25981 aaacatatgc tacagaatgg cgcttatcac gccattcttc gctgttaatt cagtaatttt
    26041 tcagaattat gcccggtcaa cgctaaaggc gatgacttca gccagtgtct ccgcgcccag
    26101 cgccaacatc accagacgat caacacctaa tgccacgccg gaacagtcag gcataccgac
    26161 tttcaaggct tcaatcagat tctggtcaat ggggtgctgc ggcaaaccgc gcgccgcgcg
    26221 cttacggtta tcttgttcaa agcgttgttg ctgctcacgg gcatccgtca attcatggaa
    26281 accattcgcc agctcaatac ctttataata aacctcaaag cgttcagcga cccgatgatc
    26341 ttcggtactg atttgcgcca gtgatgcctg gctggctgga aagtggtaca caaaggtcgg
    26401 tttttctttg ccaatatttg gctctacgcc aaaggtaaac agcaattgta gcagcgtgtc
    26461 gcggtcttct tcggtatcag caacattgct caaatccagt ttcgctgcga cttcccgcag
    26521 ttgcgttttg tcggcagaga gcgggtcaat ttccagataa cgcaagaaag cttgttgata
    26581 agaaaggctt tctgctgccg ggcagtccag cacctgttgt aagagatcgt ccacctcgtt
    26641 catcaaccgg tacatatcat agtgcggtcg ataccactcc agcatagtga actcagggtt
    26701 gtgataacgc cccatctctt cattacggaa gctgcggcac agctggaata ccggcccaca
    26761 accggcaacc agcaggcgtt tcatatggta ttccgggctg gtcattaacc agagattcat
    26821 cccctgcgaa tgcccggggc caacgaaacg tgtctcaaac gggaccaaat gaatatcggt
    26881 taccgtcgcc tggctcatac aaggcgtctc cacctccagc actccacgat cggcaaagaa
    26941 acgacggatc tccgccataa tcgccgcgcg ttttaataag ttaggaatgg atgcgctcgg
    27001 ctgccaggat gccgtttcgc tcatagttaa atctccagtt tttgacaagg gcacgaagtc
    27061 tactcgcaac gcgacggcga gacaaatttt acgcaggaat caaacagcgg tgggcagtga
    27121 ctaaaaaaag cacgatctga tggtttagta attaaattaa tcatcttcag tgataattta
    27181 gccctcttgc gcactaaaaa aatcgatctc gtcaaatttc agacttatcc atcagactat
    27241 actgttgtac ctataaagga gcagtggaat agcgttcgca gaccgtaact ttcaggcact
    27301 taccctgaag tacggggctg tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt
    27361 caagccgatc ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc
    27421 gcgcaggcaa atccaaatgc aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc
    27481 cataccgttg ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa
    27541 tatcactttc acgatacagt agcgggtggc gactggttgt gtgagcagga tgtcgtggat
    27601 tatttcgtcc accactgccc aaccgaaatg acccaactgg aactgtgggg gtgcccatgg
    27661 agccgtcgcc cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgc
    27721 acctggttcg ccgccgataa gaccggcttc catatgctgc acacgctgtt ccagacctct
    27781 ctgcaattcc cgcagatcca gcgttttgac gaacatttcg tgctggatat tctggttgat
    27841 gatggtcatg ttcgcggcgt ggtagcaatg aacatgatgg aaggcacgct ggtgcagatc
    27901 cgtgctaacg cggtcgttat ggctactggc ggtgcgggtc gcgtttatcg ttacaacacc
    27961 aacggcggca tcgttaccgg tgacggtatg ggtatggcgc taagccacgg cgttccgctg
    28021 cgtgacatgg aattcgttca gtatcaccca accggtctgc caggttccgg tatcctgatg
    28081 accgaaggct gccgcggtga aggcggtatt ctggtcaaca aaaatggcta ccgttatctg
    28141 caagattacg gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa
    28201 ctgggtccac gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc
    28261 atctccacgc cgcgtggcga tgtggtttat ctcgacctgc gtcacctcgg cgagaaaaaa
    28321 ctgcatgaac gtctgccgtt catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg
    28381 gttaaagaac cgattccggt acgtccgacc gcacactaca ccatgggcgg tatcgaaacc
    28441 gatcagaact gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt
    28501 ggtctgcacg gtgcaaaccg tctgggctcc aactccctgg cggaactggt ggtcttcggc
    28561 cgtctggccg gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagcg
    28621 gcaattgaag cgcaggcagc tggcgttgaa caacgtctga aagatctggt taaccaggat
    28681 ggcggcgaaa actgggcgaa gatccgcgac gaaatgggtc tggctatgga agaaggttgc
    28741 ggtatctacc gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcag
    28801 gaacgcttca agcgcgtgcg catcaccgac acctccagcg tgttcaacac cgacctgctc
    28861 tacaccattg aactgggcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg
    28921 gcacgtaaag agtcccgcgg cgcacaccag cgtctggacg aaggttgcac cgagcgtgac
    28981 gacgtcaact tcctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg
    29041 gagtacagcg acgtgaagat tactacgctg ccgccagcta aacgtgttta cggtggcgaa
    29101 gcggatgcag ccgataaggc ggaagcagcc aataagaagg agaaggcgaa tggctgagat
    29161 gaaaaacctg aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag
    29221 cgcattctat gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat
    29281 caaagacaac ctggcaccgg acctgagtta ccgctggtcc tgccgtatgg cgatttgtgg
    29341 ttcctgcggc atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga
    29401 ttacaccgac ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt
    29461 ggtcgatatg acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa
    29521 ctcccgcacc gcggatcagg gtactaacat ccagaccccg gcgcagatgg caaagtatca
    29581 ccagttctcc ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc agtttggcct
    29641 gaacccagag ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag
    29701 ccgcgaccac ggtaagaagg agcgtatggc gcagttgaac agccagaacg gcgtatggag
    29761 ctgtactttc gtgggctatt gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc
    29821 cattcagcag ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg
    29881 ctaaggagtg caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct
    29941 ggtggaaaaa attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcggttccgg
    30001 ctgtgtggtt cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat ggcccggaag
    30061 cctgggcggg attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca
    30121 ctctggcggc agctctgctg cacaccaaaa cctggtttga actggcaccg aaagcggcca
    30181 atatcattgt aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg
    30241 taactgtggt tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag
    30301 atgattaatc caaatccaaa gcgttctgac gaaccggtat tctggggcct cttcggggcc
    30361 ggtggtatgt ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg tattctgctg
    30421 ccactggggc tgtttccggg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag
    30481 agcttcattg gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta
    30541 caccgtatgc accacgcgat gcacgatctg aaaattcacg tgcctgcggg caaatgggtt
    30601 ttctacggtc tggctgctat cctgacagtt gtcacgctga ttggtatcgt tacaatctaa
    30661 cgtatcgcca atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc
    30721 ttatgttcaa aacgacgctc tgcgccttat taattaccgc ctcttgctcc acatttgctg
    30781 cccctcaaca aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa
    30841 agatccccgg tatggcggtg gcggtaattt atcagggtaa accttattac tttacctggg
    30901 gctatgcgga catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt
    30961 cggtcagcaa aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca
    31021 agttaagcga tcccacaaca aaatactggc ctgaacttac cgctaaacag tggaatggga
    31081 tcacactatt acatctcgca acctacactg ctggcggcct gccattgcag gtgccggatg
    31141 aggtgaaatc ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct acatgggcgc
    31201 caggaacaca acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga
    31261 agccgtctgg tttgagtttt gagcaggcga tgcaaacacg tgtcttccag ccactcaaac
    31321 tcaaccatac gtggattaat gtacctcccc cagaagaaaa gaattacgcc tggggatatc
    31381 gcgaaggtaa ggcagtgcat gtttcgccag gggcgttaga tgctgaagct tatggtgtga
    31441 agtcgaccat tgaagatatg gcccgctggg tacgaagcaa tatgaatccc cgtgatatca
    31501 acgacaaaac acttcagcaa gggatacaac tggcacaatc tcgctactgg caaaccggcg
    31561 atatgtatca gggcctgggc tgggaaatgc tggactggcc ggtaaatcct gacagcatca
    31621 ttaacggcag tggcaataaa attgcactgg cagcacaccc tgtaaaagcg attacgcccc
    31681 caactcctgc agtacgcgca tcatgggtac ataaaacagg ggcaaccggc ggatttggta
    31741 gctatgtcgc gtttattcca gaaaaagagc tgggtatagt gatgctggct aacaaaaact
    31801 atcccaatcc agcgagagtc gccgccgcct ggcagattct caacgctcta cagtaaaatt
    31861 tcatcgggtc cgaattttcg gaccttttct gcgcttttcc ttgctgtcat ctacacttag
    31921 aaaaaaacca gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct
    31981 gcatttctgg tcgttgcctg cagttctcct acgccgccgc gtggcgtaac agtagtgaat
    32041 aatttcgacg caaaacgtta tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt
    32101 tttgaacgtg gactggaaaa agtcaccgca acatacagtc tgcgtgatga cggcggcctg
    32161 aatgtcatca ataaaggcta taaccctgac agagaaatgt ggcagcagag tgaagggaaa
    32221 gcgtacttta ccggcgcacc aacacgcgct gcgctgaaag tgtcattctt tggtcctttc
    32281 tatggcggtt ataacgtcat tgcacttgat cgggaatacc gccatgcgct ggtttgcggc
    32341 ccggaccgcg actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa
    32401 caggagatgc tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta
    32461 cagcagcccg gtagttagtg agtgctgagt ttcagaccaa taatccccaa tacgattaac
    32521 gccagactcg ccaggcgcat cgggttagcg gactcaccga gcagcacaat gccggtgatg
    32581 gccgcgccga ctgcgccaat acccgtccac acggcataag ccgtccctac cggtaacgat
    32641 ttcatcgccc aggcaagtag cgccatactg actatcatcg ccgtcacggt aataacactc
    32701 ggcgtcaaac ggctaaagcc gtgggtatat ttcaggccaa cggcccatac cacttccagc
    32761 agaccagcaa taactaagat aatccaggac atatcaggct ccggagaatt ggggccgtcc
    32821 ccggtgaaaa gaggcgtttg caggtcgtcc tgcaaagctg atggttgaac ggatattttg
    32881 ccaggtggca ggggaatttc aactattacc tttgtgacta tcgcaaaaaa acaaacggca
    32941 cgacataact gtcgtaccgt tattgcttat tgctgcgctt tcgttgctgc accagaaatc
    33001 gcgttaccgc catcagaaat gtcttcacca acgccacgcg tggtgttgca ggcagttaat
    33061 actgttgaaa gcaccagaac agaaaagatc gctgcaattg tcttcttcac cataacgtct
    33121 tccttttagg taatgttatt tatgtttgcc tatagcaaat taataataga caacatcgcc
    33181 aaaaatgatg gaaatctgat gatttttcgg aagagaagaa aaattagctg gcagcgcggg
    33241 agatggagtt gccgagatgt ttgatgtctt cgccgaaacc gcgagcggtg ttacagcccg
    33301 tgagcagcgt gctggcaagc aaaacaagaa cgataaggcg tttcatcatc tctgacctga
    33361 ataagtgatg gtgcagcctg caggccgcac cacaaccgca ttacttcacg cgagagacgt
    33421 attcaccaga gcgggtatcc actttgatga cttcgccgat ttgtacgaac agcggaactt
    33481 taaccacagc gccagtagac agggtagccg gtttgccgcc agtacctgcg gtatcacctt
    33541 tcaggcccgg atcggtatca acgatttcca gttcaacgaa gttcggcgga gtaacggaga
    33601 tcggctgacc attccacaga gttacgatac actctgcctg atccagcagc catttagcgt
    33661 tgtcgccaat tgcttttgca tcagcagaca gctgctcgaa agtttcgttg ttcatgaagt
    33721 gccagaactc accgtcgttg tacaggtaag tcaggttcat atcgacaaca tcagcgcctt
    33781 cagcggaatc agtagatttg aaggtttttt ctacgcgagt accggtcagc agacgacgca
    33841 gtttaacgcg agcaaatgcc tggcctttac ccggttttac gaattcactc gcttcaaccg
    33901 cgtaaggttc gccgtctaac atgattttaa gaccagcacg aaaatcgttg ctatagtacg
    33961 ttgccataag gccctctgaa atttgttaat tggtagctaa gccacaaaat ggcgcatatt
    34021 gtaaccctaa ataccccatc cagagaagat tggttaacgc aacttgccga tgttgtgacc
    34081 gatcctgatg aacttctgcg tcttttgaat atagacgcgg acgaaaaact gttggccgga
    34141 cgcagcgcca aaaagctgtt tgccctgcgt gtgccccgct catttatcga tcgcatggag
    34201 aaaggcaatc cggacgatcc tcttttgcgt caggtactta cctcgcaaga tgagtttgtc
    34261 gtcgcgtccg gattctccac cgacccgctg gaagaacagc acagcgtagt gcctggtttg
    34321 ttgcataaat accacaaccg ggcgcttttg ctggtcaaag gcggctgcgc ggtaaattgc
    34381 cgctattgct tccgtcgcca cttcccctat gccgaaaatc agggcaacaa gcgtaactgg
    34441 cagactgctc ttgagtatgt tgctgcgcat ccggaactgg acgagatgat tttctccggc
    34501 ggcgatccgc tgatggcgaa agatcacgag ctggactggt tgctcacaca actggaagcc
    34561 atcccgcata tcaaacgtct gcggattcac agccgtctgc cgattgtgat cccggcacgt
    34621 atcaccgagg cgctggttga acgctttgcc cgttctacgc tgcaaatctt gctggtgaat
    34681 cacatcaatc atgccaatga ggtagatgaa acgttccgtc aggcgatggc taaattgcgc
    34741 cgtgtcggtg tcaccctgct taaccagagc gttctgttac gtggtgtgaa cgataacgca
    34801 caaacgctgg caaacctgag taatgcgttg ttcgatgccg gcgtaatgcc gtattacctg
    34861 catgtgctcg ataaagtaca gggcgcggcg cattttatgg tgagtgatga cgaagcacgg
    34921 cagattatgc gtgagttgct gacactggtg tcgggatatc tggtgccgaa actggcgcga
    34981 gaaattggcg gcgaacccag caaaacgccg ctggatctcc agctacgcca gcagtaactg
    35041 attaaacagg gaagttaaac attagcctga tgcgctacgc ttatcaggcc tacattgtgc
    35101 attgaaattg aatggatttg caatgaactg taggccggat aaggcgttta cgccgcatcc
    35161 ggcaagagca acaaacgcaa gtacagcaac ctgtcattaa actgggaaat ttaatctttg
    35221 cccgttgcat aacctttaat tattcatata ca
//
LOCUS       DAZFRN010000037        34308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFRN010000037 DAZFRN010000000
VERSION     DAZFRN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.037"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(114..1157)
                     /locus_tag="ACHKUB_001750"
     CDS             complement(114..1157)
                     /locus_tag="ACHKUB_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6654778.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYSEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGIKHSSIQHQ"
     gene            complement(1569..2753)
                     /locus_tag="ACHKUB_001751"
     CDS             complement(1569..2753)
                     /locus_tag="ACHKUB_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6654779.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQISLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3053..3147)
                     /locus_tag="ACHKUB_001752"
     tRNA            complement(3053..3147)
                     /locus_tag="ACHKUB_001752"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3111..3113),aa:Sec,seq:tca)
     gene            3440..4822
                     /locus_tag="ACHKUB_001753"
     CDS             3440..4822
                     /locus_tag="ACHKUB_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO6654780.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            4832..7150
                     /gene="yicI"
                     /locus_tag="ACHKUB_001754"
     CDS             4832..7150
                     /gene="yicI"
                     /locus_tag="ACHKUB_001754"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO6654781.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(7203..8912)
                     /locus_tag="ACHKUB_001755"
     CDS             complement(7203..8912)
                     /locus_tag="ACHKUB_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6654782.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDHDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(9033..10424)
                     /gene="xanP"
                     /locus_tag="ACHKUB_001756"
     CDS             complement(9033..10424)
                     /gene="xanP"
                     /locus_tag="ACHKUB_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO6654783.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            10704..11909
                     /gene="gltS"
                     /locus_tag="ACHKUB_001757"
     CDS             10704..11909
                     /gene="gltS"
                     /locus_tag="ACHKUB_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO6654784.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(12075..14156)
                     /gene="recG"
                     /locus_tag="ACHKUB_001758"
     CDS             complement(12075..14156)
                     /gene="recG"
                     /locus_tag="ACHKUB_001758"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO6654785.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIKVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(14162..14851)
                     /gene="trmH"
                     /locus_tag="ACHKUB_001759"
     CDS             complement(14162..14851)
                     /gene="trmH"
                     /locus_tag="ACHKUB_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO6654786.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGRGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(14858..16966)
                     /gene="spoT"
                     /locus_tag="ACHKUB_001760"
     CDS             complement(14858..16966)
                     /gene="spoT"
                     /locus_tag="ACHKUB_001760"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO6654787.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16985..17260)
                     /gene="rpoZ"
                     /locus_tag="ACHKUB_001761"
     CDS             complement(16985..17260)
                     /gene="rpoZ"
                     /locus_tag="ACHKUB_001761"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO6654788.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(17315..17938)
                     /gene="gmk"
                     /locus_tag="ACHKUB_001762"
     CDS             complement(17315..17938)
                     /gene="gmk"
                     /locus_tag="ACHKUB_001762"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO6654789.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            18196..19878
                     /gene="ligB"
                     /locus_tag="ACHKUB_001763"
     CDS             18196..19878
                     /gene="ligB"
                     /locus_tag="ACHKUB_001763"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO6654790.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(19875..20492)
                     /locus_tag="ACHKUB_001764"
     CDS             complement(19875..20492)
                     /locus_tag="ACHKUB_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO6654791.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(20784..21608)
                     /gene="dinD"
                     /locus_tag="ACHKUB_001765"
     CDS             complement(20784..21608)
                     /gene="dinD"
                     /locus_tag="ACHKUB_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO6654792.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(21829..22692)
                     /locus_tag="ACHKUB_001766"
     CDS             complement(21829..22692)
                     /locus_tag="ACHKUB_001766"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO6654793.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            22819..23535
                     /gene="rph"
                     /locus_tag="ACHKUB_001767"
     CDS             22819..23535
                     /gene="rph"
                     /locus_tag="ACHKUB_001767"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO6654794.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            23601..24242
                     /gene="pyrE"
                     /locus_tag="ACHKUB_001768"
     CDS             23601..24242
                     /gene="pyrE"
                     /locus_tag="ACHKUB_001768"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO6654795.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(24279..24875)
                     /gene="slmA"
                     /locus_tag="ACHKUB_001769"
     CDS             complement(24279..24875)
                     /gene="slmA"
                     /locus_tag="ACHKUB_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO6654796.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24982..25440)
                     /gene="dut"
                     /locus_tag="ACHKUB_001770"
     CDS             complement(24982..25440)
                     /gene="dut"
                     /locus_tag="ACHKUB_001770"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO6654797.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(25418..26638)
                     /gene="coaBC"
                     /locus_tag="ACHKUB_001771"
     CDS             complement(25418..26638)
                     /gene="coaBC"
                     /locus_tag="ACHKUB_001771"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO6654798.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            26810..27478
                     /gene="radC"
                     /locus_tag="ACHKUB_001772"
     CDS             26810..27478
                     /gene="radC"
                     /locus_tag="ACHKUB_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO6654799.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            27695..27931
                     /gene="rpmB"
                     /locus_tag="ACHKUB_001773"
     CDS             27695..27931
                     /gene="rpmB"
                     /locus_tag="ACHKUB_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO6654800.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27952..28119
                     /gene="rpmG"
                     /locus_tag="ACHKUB_001774"
     CDS             27952..28119
                     /gene="rpmG"
                     /locus_tag="ACHKUB_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO6654801.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            28217..29026
                     /gene="mutM"
                     /locus_tag="ACHKUB_001775"
     CDS             28217..29026
                     /gene="mutM"
                     /locus_tag="ACHKUB_001775"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO6654802.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVVTKHAQRATFYCRQCQK"
     gene            complement(29065..29544)
                     /gene="coaD"
                     /locus_tag="ACHKUB_001776"
     CDS             complement(29065..29544)
                     /gene="coaD"
                     /locus_tag="ACHKUB_001776"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO6654803.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(29552..30829)
                     /gene="waaA"
                     /locus_tag="ACHKUB_001777"
     CDS             complement(29552..30829)
                     /gene="waaA"
                     /locus_tag="ACHKUB_001777"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO6654804.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            31242..32300
                     /gene="rfaQ"
                     /locus_tag="ACHKUB_001778"
     CDS             31242..32300
                     /gene="rfaQ"
                     /locus_tag="ACHKUB_001778"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO6654805.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            32297..33421
                     /locus_tag="ACHKUB_001779"
     CDS             32297..33421
                     /locus_tag="ACHKUB_001779"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO6654806.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            33414..34211
                     /gene="rfaP"
                     /locus_tag="ACHKUB_001780"
     CDS             33414..34211
                     /gene="rfaP"
                     /locus_tag="ACHKUB_001780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO6654807.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         8194 a         8460 c         9026 g         8628 t
ORIGIN      
        1 ttgcaaagga aactttcaga aaataacccc agggtaatca cctggggaag gaacacagtg
       61 ttattattgg ataataaatt attacttgtt ttaaagttac actaatgcaa tatctactga
      121 tgctggatgc ttgaatgttt tatccctgct gcggaaaagg gcaatatcca gggccagata
      181 tattcaccgt tcatatttct gcatttctgt acaatttcct cagaaagtgt cttgtcaatt
      241 cttttccaca ggtgctcctg ctgtcttaaa atactttcgc atatttcgaa atcatcttcc
      301 tgaatcagca tttcggcgca tttaatgatt tctgatcgtt catgcagcac ggaggaatgg
      361 ttatagagcc agtttacaaa cagcaatgct tccgaatatt gtccccataa tggtgagtat
      421 gttgcatagt aatcttctgc cgtatccaga gagccggaga agtcatgcca cacagcaagt
      481 ggaaatggat gcagatttcc gcacagcatt tctgcgatgt aagtgtttgt ggcaaagcca
      541 tggcgaaaag ctgtggcggc cttaacatgt tccccgttca gaaaatgcag gagaccgagt
      601 tcataccagt aaggtgaaaa ttcatcagca tgctctttca gtacactgaa ggctcgctca
      661 tgatcgccgg tgcgtaacag ctctgaaccc aacaaccagc gagccccctg attatcttca
      721 ggattgtatg ccagtatttt atcagtgagc ataaccgcat cctgatggcg ctgtaagtgc
      781 acatttgcga gaattgctgc ataaagcgcg cgcagataag gccggttttc gggatgcatc
      841 cagattattt caccacagaa actttccggg atgatacgat tacctgccgc cagtccttta
      901 agagcggcgt tcagtgcctt acgcggggca ttctgttcca gaaatgcata tgccagatga
      961 gcgtgtatat caataaagtc cggctcccgt tgagcaaggc gtcggagttt gttgatgtaa
     1021 ctcgtctcgc tgatttcatc agcgtcgtat gcatcaagca cttcttcaaa ctcccccagc
     1081 aatgggccaa accaggtatc cggaaatacg aaataaccct tgttatcgtg cacttcaaag
     1141 cataatctat cgttcatttt ctgagcctgt aaaacatgtg gtgatatagt ctgagagcat
     1201 aaaagttgag attctgtgta tcaagtcctg tcttggcacc gaatgaatat tgcttacgaa
     1261 gccccttcat gctttccttg agaacaataa ttttatcttt atgtgaatct cattttttat
     1321 aatgatgtca gatgattgaa atgctaaatt atatctgaat caacttttcc tttgctaaag
     1381 gtatcgaaat aactacaagg ctgtattttg taattgccaa tttaaaaatg tcaatttgtt
     1441 tttaattatg tttttgtaat aagtttgaag acgcaaaaaa ataatataat aacaaaaatc
     1501 ctccagcctt gtaggccaga ggataaaata cttaattgag gaaaacatca gggaaaagta
     1561 tttctgactt aactttcccg gctggagagt gaatcaatgt aatcagcgta ccattgcatc
     1621 atttcacggc ggccatcgag atacagggca tgattatagg tacccctgat ggaattttta
     1681 tccacatgag ctaactgcat ttcaatccag gcggagttaa agccttgttc atgcagaatt
     1741 gtactcatgg tgtgtctgaa accgtgccct gtgagtcttc ccttatatcc aagtaattca
     1801 attacttgat taatgctttc cttactaatt ggtttgcggg gatcatttct accgataaag
     1861 actaggggat gatgttttga gattggttca agctgcttaa atagcataat aacctgctca
     1921 gagagcggca caatatgagg tcgcttcatc ttcattactt ctgccgggat ctcccacaat
     1981 tttgtctcaa aatcgatatc ttcccagcgt gcaaaacgca gttcttgtgt tcgtacgccg
     2041 gttaacataa tgatctgagt agctgtctta gtgattatac ttccggtata acccgccaga
     2101 tcgttgagga agtggggaag ttcattagca gtaagaaagg gaaaatgtac ttttttcggt
     2161 gtagctaaag cactggcaag atcgggggct gggttatagt ctgcccggcc agtaacaatt
     2221 gcatagcgga acacctcacc acagcgctgg cggaccttgc gcattttctc cagagctcca
     2281 cgtttttcca ttttacgcag tgcttccagt aactccattg gtttgatttc ggcaataggg
     2341 cgcttgccaa tgtagggaaa aatgtctttt tcaaaggtat caatgatttc atcacgatag
     2401 cgtaatgacc accgatcggc ttttgactgg tgccattcgc gagcaatagc ctcgaacgta
     2461 tttttcagac ttatttgctg cgcgattttt tcttcctttt ttgcctcccc tggatcgcct
     2521 ccagcagcga ggagtttcct ggcttcactg cgtttctcgc gagcatcggc tagcgttaca
     2581 tcggggtata cgccaataga aagcatcttt tctttaccag caaagcggta cttcatgcgc
     2641 cagtatttag aaccattagt gttaatcagt agatacatac cgccgccatc agcaagctta
     2701 tagggtttgt ctttgggttt tgcggttttc actttgatat cggttaatgc catgacatcc
     2761 tccggctttt gatgaattca tgttcctgag ggggatttct gccagtaact aacctgatac
     2821 ccccaataca ccccttgtat aacaaagatg ttgttaaaag ccgttgttat gtatcacatt
     2881 tttcctatta tgggggtgct tgtatttcaa taggaaataa caccccctaa agtaccccca
     2941 aaagaaactt gatacggggt gaagtaggtt gacctcagga tacaaataaa tactgatata
     3001 caatggatta gaagagattt attgacattg ggagagttca gaagaggtaa actggcggaa
     3061 gatcacagga gtcgaacctg cccgggaccg ctggcggccc caactggatt tgaagtccag
     3121 ccgcctcacc ggagacgacg atcttccgcg cctcgattgc tacatggagg cggggcgcat
     3181 tatagctact tccttgagtt tctacatccc ccagatcagt tttttccgcc taatacacgc
     3241 agcgtattat cacgaaagca ttatagttca tataaaaccc atattttaca atgatatatg
     3301 agcggaatta acgctaccac gatcacataa aacaagaatc ataaattaat aaccagatat
     3361 cggaatattc gctctcacag ggatggttta caaaatgcgt tatcggtctc agcaccccta
     3421 tagcatcaag gaaaagcaga tgaagagtga agtgttgtcc gttaaagaga aaattggtta
     3481 tggcatggga gacgccgcca gccacattat tttcgataac gtaatgttat atatgatgtt
     3541 cttttatacc gatatttttg gcattcctgc cggatttgtc ggaaccatgt ttttggtcgc
     3601 tcgtgcactg gatgcgattt ccgatccttg catggggttg ttggccgatc gaacgcgctc
     3661 tcgctggggt aaatttcgtc cgtgggtact gtttggcgca ctgccattcg ggatcgtctg
     3721 tgtactggcc tatagcacgc cagatctcag tatgaacggc aaaatgatct atgcagcaat
     3781 tacttacacc ctacttacct tactttatac cgtcgtcaat atcccttact gcgcattggg
     3841 tggtgtaatc accaatgacc cgactcagcg tatctcgctg caatcctggc gttttgtgct
     3901 ggcgaccgcg ggaggcatgc tttctactgt tctgatgatg ccactggtta atttaattgg
     3961 cggtgataat aaaccactcg gtttccaggg cggtatcgcg gtcctttccg tggtggcatt
     4021 catgatgctg gcattttgtt tcttcaccac taaagaacgc gttgaagcac cacctacaac
     4081 aacgtctatg cgggaagatt tacgtgatat ctggcaaaac gaccagtggc ggattgtcgg
     4141 tttactaacc attttcaata tcctggcggt gtgcgtacgc ggtggggcga tgatgtatta
     4201 cgtcacatgg attttgggca cgccggaagt gtttgtcgct tttctcacca cttattgcgt
     4261 gggtaacctg attggttccg cactggcaaa acctctgacc gactggaaat gtaaagtcac
     4321 tatcttctgg tggacgaacg ccctgctggc agtgattagc ctcgcgatgt tctttgttcc
     4381 catgcaggcc agcatcacta tgtttgtctt catcttcgtg attggtgtgt tgcatcaact
     4441 ggtgacacct atccagtggg taatgatgtc cgataccgtc gactacggcg agtggtgcaa
     4501 tggtaaacgc ctgaccggga tcagttttgc tggcacgctg tttgtgctca aactggggtt
     4561 ggccttcggc ggcgctctta tcggctggat gctggcttat ggcggatatg atgcggcaga
     4621 aaaagcgcag aacagcgcca cgattagcat cattattgcg ctattcacga ttgttccggc
     4681 gatctgttat ttgctgagcg cgattatcgc taaacgctac tactcactca cgacgcacaa
     4741 tctgaaaacc gttatggaac agctggctca gggtaaacgc cgttgccagc aacaattcac
     4801 ctctcaagaa gtgcagaact aaggaacggc aatgaaaatt agcgatggaa actggttgat
     4861 tcaacctggc ctcaatttga ttcacccgct tcaggtgttc gaggttgaac agcaggataa
     4921 tgaaatggtg gtctatgctg ccccccgtga tgtgcgtgaa cgtacctggc agcttgatac
     4981 tcctttattt acgttgcgct ttttctcccc acaggaaggt attgtcggtg tgcggattga
     5041 gcattttcag ggggcgctga ataacggtcc tcattatccg ctcaatattt tgcaggacgt
     5101 gaaggtcaca atcgaaaaca cagaacgtta tgctgagttt aaaagtggca acttaagcgc
     5161 gcgtgtcagc aaaggtgagt tctggtcact ggattttctg cgcaacggcg aacgtattac
     5221 cggtagtcag gtgaaaaata atggctacgt gcaggacacg aataatcaac gcaattatat
     5281 gtttgagcgg cttgatcttg gcgttggcga aacagtttac ggtctgggag agcgctttac
     5341 tgccctggtg cgcaatggcc agacggtaga gacctggaac cgggacggcg gcacaagtac
     5401 tgaacaggcg tataaaaata tcccgttcta catgactaac cgtggttatg gggtactggt
     5461 caatcatccc cagtgtgtct cttttgaagt gggatcggag aaagtctcca aagtgcagtt
     5521 cagcgttgag agtgaatatc tcgaatactt tgttatcgac ggcccgacgc cgaaagcggt
     5581 acttgatcgt tatacccgct ttactggtcg tccggcgctg ccgcccgcgt ggtccttcgg
     5641 cctgtggcta accacttcat ttaccaccaa ctacgacgaa gcgacggtaa acagctttat
     5701 cgatggtatg gcggaacgca atctgccgct gcatgttttc cactttgact gtttctggat
     5761 gaaagccttc cagtggtgcg attttgagtg ggacccgctg actttccctg acccggaagg
     5821 gatgatccgc cgcctgaaag cgaaaggact gaaaatctgc gtctggatta acccctatat
     5881 cggtcaaaaa tcccccgtct ttaaagagtt acaagagaaa ggctatttac tcaaacgccc
     5941 ggacggttcg ctatggcagt gggataaatg gcagccaggt ctggcgattt atgactttac
     6001 caatccggat gcctgcaaat ggtacgccga caaactgaaa ggtctggtcg cgatgggcgt
     6061 tgattgcttt aagaccgact ttggcgaacg tatcccaact gatgttcagt ggtttgacgg
     6121 ttccgatccg cagaaaatgc ataaccatta tgcgtacatc tacaacgaac tggtgtggaa
     6181 cgtgctcaag gacaccgttg gtgaggaaga agctgtcttg tttgcccgct cggcctccgt
     6241 cggtgcgcag aaattcccgg tacactgggg tggcgattgt tacgctaact acgaatcaat
     6301 ggcggaaagc ctgcgcggtg gtttgtctat tggcctttca ggttttggct tctggagcca
     6361 cgatatcggc ggctttgaaa ataccgctcc ggcgcacgtt tacaaacgct ggtgcgcgtt
     6421 tggtttgctc tccagccata gccgtttaca cggtagcaaa tcttatcgtg tgccgtgggc
     6481 ctacgatgat gagtcctgtg atgtggtgcg cttcttcacg caactgaaat gccgcatgat
     6541 gccgtatctg tatcgtgaag ctgcgcgtgc gaacgcgcgg ggtacgccga tgatgcgggc
     6601 catgatgatg gagttcccgg acgatccggc ttgtgattac cttgaccgtc aatacatgtt
     6661 aggcgacaac gtgatggttg cgccggtgtt cactgaagcg ggcgatgtgc agttctacct
     6721 gccggaaggt cgctggacac acctgtggca caacgatgag ctcgacggta gtcgctggca
     6781 taaacagcag cacagcttcc tgagtctgcc catttatgtg cgtgataaca ccctactggc
     6841 gctgggcaac aacgatcaac gtcccgatta cgtgtggcac gaaggcacgg cattccacct
     6901 ctttaatctg caagacgggc atgaagccgt ctgtgaagtg cccgctactg acggatcggt
     6961 gatctttact ttaaaagcag cacgtactgg caacacgatt actgtgactg gtgcgggcga
     7021 ggcgaagaac tggacgctgt gcctgcgcaa tgttgtgaaa gtaaatggtc tgcaagacgg
     7081 ttcgcaggct gaaagtgagc aggggctggt ggtgaagcct caagggaatg cgctgacaat
     7141 tacgttgtaa tctattgatg gaatgccggg tacgtctgag tcgtacccgg cgagattgat
     7201 gtttaccgca ccggctccgt cgatgtaact tcaccgctgg aaacgatgcc agcattcatg
     7261 gtttgagtca cttgctgctt tgcggcgttc acggcatgca attgcccgct aaccgtaggt
     7321 ttcaacggga cattggcctg aatatcgcca ctggcggtaa gctgaatatt gccgtcgcca
     7381 gtcaacggta atttaggcca tccccactgt tgcaaaatat tcaccggcac accgcgtcca
     7441 ttcaggctga tatgtgtctg acgttgtggc gtttgtgaaa cactggcggt ggcttccaga
     7501 atgccttttt cagtaaatgc actcagttcg ctgatattca ccgtgctgct gttggcggtc
     7561 agcgccagcg acgggcgacg aacatcaaca cgattgaatg tcgcggcggc ggcattcaga
     7621 ttcgccgagc cactccagac gccccattta tgatcggtaa ccagcgtcag gttggcaccg
     7681 taaccatcga gcgcggtgag ctgccacggg aagtcagggt cgatatcaat gatcagattg
     7741 cggctggcgc taaatctctt cagttgcagg ctgtttaacc aaccgggtgt cgtttccatc
     7801 cacaactgtt gccagttttt cggcaaggta tattccagcc cggcaattgc cgcatcatca
     7861 aggatcaacg ttttcccgtc acgcagccag ttccctgacg ttctgaccat acccccttcc
     7921 cagcggctgg tgaactggcg cagcgctacg ccctgcgggg aaaattccgc gtttataatc
     7981 gggtcaaata aatgcagcga accataaatg aactcgctgg cgttcatcga cagtttgcct
     8041 tcttgtgtct gccagtcatc tttactgaag gtcatgttgc gcaagctgag atcgtggtcg
     8101 gtcaccgccc agtccggacc ttgcaaacga gcatcgatca cttccaggcg accaatctgc
     8161 aacgacggga cagagcgtaa tggcgcaaag aagtcggtta gcgatttttc gctttgtaga
     8221 cggatatccg ccatgcgcag attttccact tgccagctgc cgtcggcgtt acgctgcgcg
     8281 tttccggtta atgtcccgcg ggcgatgtcg gcacccaggt tagtcagcgt aacgcgatcg
     8341 ttatcaatac tgccttcaat cagtacattg gtggcaggaa catcattgag cgaaagcgat
     8401 ccggcactaa actgaatctg cgccttcgta cccagcactt taccggcttc tggtgaccac
     8461 ggaaccacgc cgccatttac ccgctgcgcg ctcagtttcc attcgctatt cgggctatta
     8521 aacgccatat cacgcagttg cagacgatcg gctttgaacg gtagcggcgc ggtctggtcg
     8581 gtgagattca gcgtcccgtt ttccagcagg atggtatcga catggcgtgg ttcggtcagt
     8641 tgccgactgc ttagcgcaat gtcgacactt tttgccacca gggtcgcggg ctgaccatca
     8701 cgaccaaacg tgacgttctc cagcacgata tgagatggcg cggaaaaacg gtgatccatc
     8761 gccccgaagg ccagatgata gtcgctattc tcggaaaccc atgcgctgat atgttctgct
     8821 ccccagcggg tttgcagaag aaaatagagg ccagcgatca ccactaacag agcgatgaga
     8881 atgtagagaa gcagcttccc aataaatttc atggtcttcc atcccgtgaa atgcacataa
     8941 gggagttatg cacgatttac gcgcaatcct caaggcggga atggtgaaag aggtgagact
     9001 gcggcggtaa ttatcttacc gccgccagtg aattactgtt tttctggtgg gaaaatcaga
     9061 ttcagcacga tggcagtaat accgcccgcg gcgatcccgg aggagagcag gtttttcagc
     9121 cattcagggg caaactgcaa aatcagcggc tgctgagaca cgcccagacc aaccgccagc
     9181 gacagcgcga taatcagaat cgcccgacgg ttcagcggct cacgagaaac gatacgcaca
     9241 ccggaggcgg cgatggtgcc aaacattaca agcgttgcgc cgcccagaac cggttctgga
     9301 atgtgttgta caaaaccgct cactgccggg aacagaccca gcacgatcaa catcagcgcg
     9361 acgacaaaac cgacatagcg gctggcaaca ccagtcaact ggatcactcc gttgttttgc
     9421 ccgaagcagg agttcgggaa ggtgttaaac accgccgaaa caaacgagtt caggccgttt
     9481 gccagcacgc cgcctttcag gcgtttcatg tacagcggac cggacactgg ctgttcggaa
     9541 acgtcagagg tcgccgtgat atcgccaatg gtttccagcg aagtgatcat aaagaccagc
     9601 atcagcggca gcagcagact ccattcaatg ccaagaccgt aatagagcgg cgttggcacc
     9661 ataatcagtt cttgcgtcat cggttcgttg ctttctggca acatgcccat aaaccacgcc
     9721 agcgcatatc cggccgccat cgcaatcacc agtgaggcca cgcgtaagta agggttacgt
     9781 tgacggttaa gcaggataat taaggctaag accacgcctg ccagcagcag atttttcggt
     9841 gcgccgaagg tgttatcgct catggctgcg taaccgccgc caatggacgt taacccaacc
     9901 tgaattagcg acaggccgat aatcatcacc acaacgccag aaaccagcgg cgtaataatg
     9961 cggcgcgcca gatgcagaac gcgggagatc accatctcgg tgcaacttgc cagcatcaac
    10021 gtgccgaaca aagccgccat catggtagga acatcagcac caccggtttt cagcgcggta
    10081 ccgcccataa tcagcggggc aacaaagttg aagctggtgc cctgaataga caacagcccg
    10141 gagccaaccg gaccccaggc cttaatttga ataatcgatg ccacaccgga ggcaaacagc
    10201 gacatactaa taatgtgttg cgtgtcttgt gccggtaaac ccagcgcctg gcagattaat
    10261 agcgctggcg tgatcaccgc aacgaacatc gccagcagat gctgacaggc ggcaaacagg
    10321 gtttgaggaa gcggcggacg atcttcaaga cggtaaatca gttcgctgtt ttgagtctgc
    10381 gcaaccggtt gcgcattttc tgactcgagg gtggaaacag acatcgaaaa caatcccgtg
    10441 gtagcaaagc gggcatttta gctgaccgaa agagaaaagc aaacgtttgc cacatcctca
    10501 tctaaatatt taaatgagat aaatgcactt ttttatataa cttttggtat ttttctgctt
    10561 aaaatccatg ccatttgttc attaacagct gagaatttga cccaggaaag cataactcga
    10621 tacccgtcat agttcatgaa gttgccactg cgcgatgaag tatgacgagt atgaaagagt
    10681 gatgcggata caaaggagta actatgtttc atctcgatac tttagcaacg cttgttgccg
    10741 caacgctgac gttgctgctc gggcgtaagt tggtccattc cgtctccttt ttgaagaaat
    10801 acaccatacc ggaacctgtt gcgggtggtt tgttggtggc gctggcgcta ctagtactga
    10861 aaaaaagcat gggctgggaa gtcaactttg atatgtccct gcgcgatccg ttaatgctgg
    10921 ctttcttcgc caccattggc ctgaacgcca acattgccag tttgcgtgcc ggtgggcgtg
    10981 tggttggcat cttcttgatt gtggttgttg gtctgttggt gatgcaaaat gccattggca
    11041 ttggtatggc tagcctgtta gggcttgatc cgctgatggg gctgttggcc ggttctatta
    11101 cgctttccgg cggtcacggt acgggcgctg cgtggagtaa attgttcatt gaacgttatg
    11161 gcttcaccaa tgcgacggaa gtggcgatgg cctgtgcaac gttcggtctg gtgctgggcg
    11221 gcttgattgg cggtccggtg gcgcgctatc tggtgaaaca ctccaccacg ccgaacggta
    11281 ttccggatga ccaggaagtc ccgacggcgt ttgaaaagcc ggatgtggga cgcatgatca
    11341 cctcgctggt gctgattgaa actatcgcgc tgattgctat ctgcctgacg gtggggaaaa
    11401 ttgttgcgca acttttggct ggcactgctt ttgaactgcc gaccttcgtc tgtgtactgt
    11461 ttgttggcgt gattctgagc aacggtctgt caatgatggg cttttaccgc gtctttgagc
    11521 gtgcggtatc cgtgctgggt aacgtaagcc tgtcgttgtt cctggcgatg gcgctgatgg
    11581 ggctgaaact gtgggagctg gcttcgctgg cgctgccgat gctggcgatt ctggtggtac
    11641 agaccatctt catggcgttg tatgccatct tcgttacctg gcgcatgatg ggcaaaaact
    11701 acgatgcggc agtgctggct gcgggtcact gtggttttgg cctcggtgca acgccaacgg
    11761 caatcgccaa catgcaggcg atcactgaac gctttggccc gtcgcacatg gcgtttttgg
    11821 tggtgccgat ggtcggtgcg ttctttatcg atatcgtcaa tgcgctggtg attaagctgt
    11881 atttaatgtt gccgattttt gccggttaac cgatgaagcg gcggtagaag tgccgccgca
    11941 acaaagacaa atgcctgata cgcttcgctt atcaaggctg cgcattgatt cacaatttat
    12001 tgaatttgta gggtgaatcg catccggcag gaaggtaggg taacctgaaa tggcggtctt
    12061 ctcactgccg ccttttacgc attcgagtaa cgttctgtct ccggcatcca gcgttctatc
    12121 aaggcttttg cctgttgtgg gtaacgttcg tgaatatgtc gcgccaggcg ctgaacttcc
    12181 gggatcatcg cctgatcgcg cagtaaatcc gccactttaa attcagcatt acccgtctga
    12241 cgcgtgccta acaattcgcc agggccgcga atctccagat ctttttgcgc aatcacaaaa
    12301 ccgtcgttac tgtcgcgcag cacttgcagg cgaatttgcg ccgttttaga aagcggcgtt
    12361 ttgtagagca gcacgcagtg agaagccacc gcgccacgac ctacgcgccc gcgcagctgg
    12421 tgtaactgcg ccagacccag acgctcaggg ttttcgataa tcatcaggct ggcgttaggc
    12481 acatcaacac cgacttcaat aacggtagtg gcaaccagca gatgtagctc accttgttta
    12541 aacgacgcca tcaccgcctg tttctcggct ggtttcatcc gcccgtgtac caggccaaca
    12601 ttcaattctg gtagcgccag tttcaactct tcccaggtag cttccgccgc ctgcgcttcc
    12661 agtaactctg actcttcaat caacgtacaa acccagtacg cctgacgacc ttcagttatg
    12721 caggcgtggc gcacgcggtc aatgatgtcg gtacggcggg tatcaggaat agcgaccgta
    12781 gtcactggcg tgcggcctgg cggcagctca tctatcaccg aggtatcgag atcggcatac
    12841 gccgtcattg ccagcgtgcg ggggatcggc gtggcggtca tgatcaactg atgcggatgg
    12901 aagccctgct gctggccttt ctcccacaat gccagacgct gatgcacgcc aaaacgatgc
    12961 tgttcgtcga taatcaccag cgccaggccg ttaaactgca cctgttcctg gaagatggcg
    13021 tgtgtaccga caatcatctg cacctgaccg ctggcgatgg cttcctgctg agacagtcgc
    13081 gctttgcctt tctgcttacc ggcgagccag cccactttga taccgagcgg tgcaaaccag
    13141 ttgcggaagt tattggcgtg ctgctcggcg agtaattcgg ttggtgccat cagtgccacc
    13201 tgtttgccgt gggcaatcgc acgcagcgcg gcgagggcgg cgaccagcgt tttaccggaa
    13261 cctacatcgc cctgtaccag gcgcatcatc ggcacatcca gcgccatatc atgctcgatc
    13321 tccgccacta cgcgtgcctg tgcgcccgtt ggcttgaacg gtaaggcggc gaggagttta
    13381 tttttcagcg tgtcattggc gctcagcggc tgggcatgaa aacgctgtgc tccggcacgt
    13441 aaggctaaca tgctgaggtt gtgcgccagc agttcttcca gaatcagacg acgttgcgcc
    13501 ggatgctgcc cggtttccag atcgctaagc tgtagcgtcg gtggcgggcg gtgcaaagtg
    13561 cgcaacgctt ccggtagcgt catcattcct tgtgacagtt ccggcggcag gagttcttca
    13621 atggcgcagg tgtcgagcag atccagcgcc tggtcggtta atttacgcag cgtggcctgc
    13681 tttacgcctt ccgttgttgg ataaaccggc gtgagcgttt cctgtaattc tggcgtgctg
    13741 agatcgccct gcacgcggta ttccgggtgg atcatctccg caccatattt accgcgcttt
    13801 gcttcgccat aagccagtac acggcggccc gtcgccaggc tatttttcat tgccgcgctg
    13861 aaattgaaaa agcgcatggt gaggatgccg gaaccgtcgc tgatctggca ggtcatcatc
    13921 cgccgaccgc cgaaggagat attgcagttc agcacttcgc cttccaccgt ggcataaacg
    13981 cccggcagta gttctccgat ggggtagaga tgggtgcgat cttcgtagcg caggggaagg
    14041 tgtaagagta gatcctgcac ggtatgcagg ttgattttcg ccagtttgtt actaagtgct
    14101 gcgccaacgc ccgttaggga actgagtggg acagcatcta acaggcgacc tttcatggca
    14161 cttaccctgc agcctgcata gtagcccacc agtcggcatc agcttcgatc tcgccttgct
    14221 gattgacgtg gggataaggc aggcctttgc gttttgcgac tttcgccagc accggatagc
    14281 cgccttcaaa caacaggcgt tgttgctctg cttccggcaa catgctgttt tcacgcaggt
    14341 acatgcctgc attttgccgc tgacgctggg cttcgtaaag aatgagggct gaggcaacgg
    14401 aaacattcag cgactgcacc atgccgatca tcggaatgat gatgtcctga tccgccaggg
    14461 ccaatgcttc ctgcgtgatg cccgttttct cctgtcccat caaaatgcag gtcgggcgag
    14521 tgtaatcaat ttcgcggaaa tcgacagcgt tatcagaaag atgggttgcc agaatctgca
    14581 tgccccggcc tttcagatga gcgacggcat cgccaatggt gcggtgtgtt ttcacctgta
    14641 cccagctgtt actacccgcc gctgccgaag ccatggtgcg catgcggcta ccaggccaga
    14701 cggcgtgaac ttcatgtacg ccaacggcat ctgcggtacg aataatcgca gaaacgttat
    14761 gaggtttgtg gacctgctcc atgcagacgg tcagatcagg ctgccgcctg gcgagcattt
    14821 cgcagatgcg tgcataacgt gttgggttca taaaacatta atttcggttt cgggtgactt
    14881 taatcacgtc tggcatcacg cggattttgc gcatgatatt cgccagatgc acacggtcac
    14941 gagcggtcag acgaataaag gcgctgtaga cgcgaccatc tttctcttcc gtattcaaac
    15001 tttgaatatt cgaagtcgtg gtgttaattg ccgccgtcag gtttgccagc gcaccctgat
    15061 gattgaacat ctccaccttg atttcggtga tgaactcctg cgccgtctct ttatcccatt
    15121 ccacagccat aaacttctct ggctctttct ggtagccacg gatattacgg caggattcat
    15181 ggtggatcac cagaccttta ccggggctga cgtgggcgat aatcgggtcg ccaggaatag
    15241 ggcggcagca tttcgcaaag gtgatcagca cgccatcggc acctttaatg ggcagatgtc
    15301 cgtggctttg ggttgccggt ggaatggagg cgtccccatg ttgcagattt ttcgcgacca
    15361 ccacgctcat tgcgttacca agtccgattt ctgccagcag atcgtcaagc gttgccagct
    15421 tcatgcgatc cagctcgcgc tgaatatttt cctgcgggat ttcattgagc ttacggctac
    15481 cacccaaagc atggttgagc agacgacggc ccaggcttac agaatcatca cgcttgaggt
    15541 ttttcagcaa ctgacgaatt ttggcgcgcg ctttcgagct aacgacaaag ttcagccacg
    15601 cggcattcgg gcgagcgccc ggagcggtaa tgatttcaac ggtttgaccg ctggtaagcg
    15661 gctgcgacag cgggtaaggc tggcggtcaa cgcgtgcgcc cacgcaggca tggccgatat
    15721 cggtatgcac tgcataagcg aagtcgacgg gcgttgcacc ggcaggcagc tcgacaatgc
    15781 gcccttccgg tgtgaaaacg taaatctcat ccgggaagag atcggattta acgctctcga
    15841 taaattcaaa cgaactaccg gcgctctgtt gcagctccag caggctttgc atccagcgct
    15901 gggcgcggat ttgtgcggta gtactggttt cgccgtgctc tttataagcc cagtgcgcgg
    15961 caacacccat ctccgccatc tgatccatat cttcggtacg gatctggacc tcaaccggca
    16021 cgccgtgcgg gccaatcatc gaggtgtgca acgactgata gccgttcgct tttggaatgg
    16081 cgatatagtc tttcacgcgg cccggacgcg gcttgtacag gctgtgcatc tggcccagca
    16141 cgcgataaca ggtgtcagaa tcattgacga tcacgcggaa agcgtagatg tccatgattg
    16201 agtgaaaacg ctgctctttg agcaccattt tgcagtaaat cgaataaaga tgcttttcgc
    16261 gaccactgac gcggcacggt attcccgctt cctgcaaacg cccttcgatt tcagagagga
    16321 ttttttggat catctcttta cggttgccgc gcgcggcttt caccacttct ttaattacgc
    16381 ggtaacgatt gggatacagc gcctcaaaac ccagctcttc gagttcggtt ttaatgtggt
    16441 ggatacctaa acggtgcgcc agcgggctat aaatttcgag agtttcacgg gcgatgcggc
    16501 gacgtttgtc cgggcgaagt gagcccagcg tgcgcatgtt gtgggtacgg tcggcaagtt
    16561 tgatgaggat gacgcggata tcctgcacca tcgccataat catcttgcga aagttttcgg
    16621 cctgcgcctc tttcttatcg cggaacttga gtttatcaag tttcgacacc ccctctacca
    16681 gctcggcgac gcttttacca aaaagctgtt ccatatcctg gtaggtggcg ggagtatctt
    16741 caatcacgtc atgcagcagc gccgccatca gcgtttcata gtcgagtttc atctcggcca
    16801 gaatgcaggc aaccgctacc gggtgcgtga tatagggttc accgcttgaa cgtgtttgcc
    16861 cctcgtgagc atcacgtgca acgagatacg cctgccgcag acgcttgatt tggtcttccg
    16921 gcaggtaggt ttgaatcagt tgattcaggc tttcaaacag atacaagggc gacccgcttt
    16981 atgattaacg acgaccttca gcaatagcgg taacggcttg taattcagcg gcttcctgct
    17041 cttgctgttc ctggcgttcg cgaacgtcga ggatctggtt gttgatcaga ccttcttcga
    17101 tttcgcgcag cgcgattaca gtggttttat cgttttcttc cggtaccagc ggatcctttc
    17161 cgcctacctg catctgacga gcgcgacgcg cggcgaccag taccaggtca aaacggttac
    17221 caattttctc tacagcgtcc tgaacagtta cgcgtgccat acttaaaaag ctccacaggt
    17281 gaagaaatga ctgggcatga tactgaaatc aggttcagtc tgccaacaat ttgctgatta
    17341 aagcgtcatg acgctgcttt tggcggctca tgcgcagacg ttcggcgcga ataatggtct
    17401 tcaaatcggt caacgcggta tcgaagtcat cattcacaat cagataatca tattcggcgt
    17461 aatggctcat ttctgcaaca gcttgcgcca tacgctttgc aatgacctct tcgctgtcct
    17521 gaccgcgacc gcgtagacgg cggtccagtt caattttgga cggcggtaaa ataaagatac
    17581 tccgcgcgtg cggcattttc tggcgaattt gctgcgcgcc ctgccagtcg atatcgagaa
    17641 aaacatcgac accggtcgcc agtacttgct caatggcctc acgcgaagtg ccatagtaat
    17701 taccaaaaac ttctgcgtgt tcgaggaacg catctctgct aatcatttct ttaaattcat
    17761 catgattaac aaagaaataa tgttcaccgt ggacttcacc aggacgcggt tggcgtgtgg
    17821 tgtgtgaaac agaaacctgg gtgtcataca acggttgggt ttttaataaa gcctgaatca
    17881 ggctggattt acccgcgcca ctgggggcag aaacaatata aagcgtgcct tgagccatga
    17941 gtatctttcg tatgtgatta gcgaaataaa gcctacatac gagcttatta tacacggcgc
    18001 ggccatgtga cgtagccctt gctcacactt tttgcgcaaa gttgttgcgt tttgcgtagc
    18061 tgtttccgca tttgcctgtg atttactgca ataaagacga ttctcaggaa gcgtcctcgc
    18121 agactccgtt tttgccactc ggctttgcct tcaccacgtt gtatatctgc cagcctgact
    18181 acagcggagg tgaagatgaa agtatggatg gcgatattaa taagtatctt gtgctggcaa
    18241 tcatctgtgt gggcggtctg tccggcctgg tcgccagcca gagcacagga agaaatttcc
    18301 cgcctgcaac agcaaataaa acagtgggac gatgactact ggaaggaagg aaaaagtgag
    18361 gtggaagacg gtgtttacga tcagttaagc gcccgtctta cgcagtggca acgctgcttt
    18421 gggagcgagc cccgcgacgt gatgatgccg cctttaaatg gtgcggttat gcatccggtt
    18481 gcccatacgg gtgtacgcaa aatggtggat aaaaacgcat taagtctgtg gatgcgagaa
    18541 cgtagcgatc tttgggtgca gccaaaagtt gatggcgttg cggtaaccct ggtttatcgg
    18601 gacgggaaac tgaacaaagc aatcagtcgc ggtaatggcc tgaaaggcga ggactggacg
    18661 cagaaagtta gcttaatttc cgctgtgccg caaaccgtta gcgggccttt agccaacagt
    18721 acgcttcagg gggaaatatt tctccagcgc gaggggcata tccaacaaca aatgggggga
    18781 ataaatgccc gcgcaaaagt tgctggcctg atgatgcgcc aggacgatag cgacacgctg
    18841 aattctctgg gcgtttttgt ctgggcatgg ccggatggac cgcagttaat gaccgatcgt
    18901 ttaaaggagc tggctaccgc aggttttact ctaacgcaga ggtatacccg tgcggttaaa
    18961 aatgctgatg aggttgcgcg cgtacgcaat gagtggtgga aagcgaaatt acccttcgtc
    19021 accgatggcg tggttgtgcg aggggcgaaa gaacccgaat cccgccattg gctaccaggc
    19081 caggcagaat ggctggtggc ctggaaatat caacctgtag ctcaggttgc cgaagtgaag
    19141 gcgattcagt ttgcggtggg taagagcggt aaaatatcgg tggttgcgtc actcgcacct
    19201 gtcatgctgg atgataaaaa agtccagcgg gtgaatattg gttccgtcag gcgctggcag
    19261 gagtgggata ttgcgcctgg tgatcagatc ctcgtcagcc ttgccggtca gggtattcct
    19321 cgcattgatg atgtggtctg gcgcggtgca gaacgtacaa aaccgacacc accagaaaac
    19381 cgctttaact cgttgacctg ctactttgct tctgatgttt gtcaggaaca gttcatttca
    19441 cgcttagtct ggctggggtc aaaacaggtt ctggggctgg atggcattgg tgaggccggc
    19501 tggcgcgcgc tgcatcagac tcatcgcttt gagcatatct tttcctggct tttattaacg
    19561 ccagagcaat tacagaacac gccggggatc gcgaaaagta aaagtgcgca gctatggcat
    19621 cagtttaatc tggctcgtaa gcagcctttt actcgctggg tgatggcaat gggaataccg
    19681 ctaacccggg cggcgcttaa tgccagtgat gaacggtcct ggtcgcaact tttatttagc
    19741 acggagcagt tctggcagca gctgccgggg actggatcgg ggcgcgccag acaggttatt
    19801 gaatggaagg aaaatgcgca aatcaagaag ctgggcagtt ggctggctgc ccagcagatc
    19861 acaggttttg aaccttagtg tccttcgtgg ctgtagtaaa agaccggtaa tcccagctta
    19921 aggcgtagcg ccagtaagcg ggcaaaaaag ccgaatacca gggtggagat aattaccaca
    19981 tcatggttag aaacatagtg ttgcagcgcg atgtacagca cggcggaggc aaatgagaca
    20041 ccggcataca actctttctg gaataccagt gggatacgtt tacagaacat atcgcgcaaa
    20101 acgccgccaa acacgccggt ggttaccgcc gcgacaacgg caataattgg gccgtgcccc
    20161 atatccagtg caacctgtgc cccgatgata gaaaagacca ccagtccgag cgcatcgagc
    20221 accagaaaca ctttgcgcag gtaaggcatt acaggagcaa cgatagtggt gagtactgcg
    20281 gcggtagcga cgataatcac atattccggg tgtttgaccc agccgagcgg atagtggccc
    20341 agcagaatat cgcgcactga ccctccgcca attgcggtgg cggtcgcaat aataattacg
    20401 ccaaatgtat ccatgcgccg tcgtccggcc gccagcgccc ccgtcatggc ttcggcagta
    20461 atgccaacca aatacaaaat gtgtaacagc atggttctct ccctaaaaat ctgtctggca
    20521 gggtagcgat ttgtgcgcat tgtcacgatt gagatttttt aaggtgaggg gaaattgata
    20581 acttgtagtt atgtgaaatc gctattttct gtagcgtaga tgcattcttc tgacttcttg
    20641 tctgagaaaa tgcataagaa aaaataggtt aaaattgagg ggtggaaaag aaaggtgcaa
    20701 tgtttagcca ttgtgttcac cgcacctgga ctttggctta cgcatcgggt atctcaatca
    20761 acaaaaagag tttttagttt ctgctatttc ttctctgtaa tttttacact gttttccaac
    20821 tgcttgatgc tgacctgcgg ggtcggcaac tcctcaggca tggttccgcc aagttcctga
    20881 atggtttgcc tcaccttgct acccacgtca aagtgtgtgg tattagcttg ctgttttgaa
    20941 ttcacgccat cccgcttgag tttttcttct gtttgggtag ctcgaaagag attagccgcc
    21001 agttctgttg aacccatatg atccaggatc ttctgactct ttttcagccc cttccgctga
    21061 tggatggctt tctgatctaa tccaccatac agcccccggt aaccatgatt ctgaaatatg
    21121 gcgaaatcga tagctgtcgc taccccagct tgctgcgctg cctcaaccaa ttgtttgtta
    21181 tgttctttca attcatttct cagaaaaaga cgcttttcgt cttcacgaag ttgcctgaaa
    21241 gcttcatcgt cagccagctc ctgccgtcgg gtctggatag caaaataagt ttgccctgcc
    21301 gcaataaccg gtttcgcagg gtcgccgttt tgtactacca ggtaacaggc atagcgggag
    21361 agatgaacat cttcaagttc tcgttgagcc cctgaaccta atacaaccat tttggtggtt
    21421 tcaacgaaat ggtcagatgc agcttgattg ctggcttcgc acgcttgagt cgcgcgggcc
    21481 agtacctttt gaaaattgcg ccagtcccga taatccagta acggtgccaa ttctctagct
    21541 gaccaaaatt cttgcccttc agtgccataa tgccgtattt cttcaaaagg ttgatgatgt
    21601 tcgttcattg cccactccat gtgatatcga ttgcaaagaa agtaaatctg taactgtatt
    21661 tatatacagt tgtgagtatc tcacgttctc agtgtgctgg gcaaaaaaac atcgcaaaat
    21721 ctggagcagg cacgcaaaac agaattgaag ttgcggggaa gaaaaatgcc gctctgtttg
    21781 agtgggcggc atttgtgaag gctgaaaagt aagaattggc gttacgagtt attcaatgtt
    21841 ctgaatctgc tcgcgcatct gctcaatcaa cactttcagc tcaatcgcgg agtttgtcac
    21901 ttcggcattg atagacttcg acgcaagagt gttcgactcg cggttgaatt cctgcatcat
    21961 aaagtccaga cgacgaccaa cggcttcttt tttcttcaga atgttgtagg tctctttgac
    22021 atgcgcttcc aggcggtcca gttcttcggc aacgtcaatt cgttgtgcca gcagaaccag
    22081 ctcctgctcc agacggttgt tttccaactg aacctgagca tcttccagct tcgcgaccag
    22141 acgctcacgc tgccattgca ggatttccgg catatgggcg cggactttga ccacttcggc
    22201 ggtgacgcct tccagacgct gctcgatcaa tgctttcagt gcctgacctt cggtttcgcg
    22261 cgcgacaata aagtcgtcca gcgtaccatc cagcgccgcg agaatttcag cggcaatggc
    22321 gtcaagatcc tgctcctggg ctgccatcac gcccggccag cgtagaatat caaccgggtt
    22381 gatttcccct tcgtcactct gcatttttac ccagttcgcg gcagttacca gctgtttagc
    22441 cagtttttcg ttgaggatca gctcaccttg cgcgctaaca tctggctcat agcgcagggt
    22501 acattccact ttaccgcgcg tcaggcgaga acgaatacgc tcgcgaacga caggttcaag
    22561 gctacggaac tgctccggca gacgaaagta agtttccaga taacgctggt ttaccgagcg
    22621 catttcccag gttgcgctcc cccattcacc cttgatttca cgccgggcgt aggcggtcat
    22681 actgcggatc atagacgttc ctgtttataa aaggagaggt ggaaggatta tagccatcga
    22741 tgccttgtca ggataggaat aaccgccgga agtccgtata atgcgcagcc acatttgttt
    22801 caagccggag atttcaatat gcgtccagca ggccgtagca ataatcaggt gcgtcccgtt
    22861 accctgactc gtaactatac aaaacatgca gaaggctcgg tgctggtcga atttggcgat
    22921 accaaagtgt tgtgtaccgc ctctattgaa gaaggcgtgc cgcgcttcct gaaaggtcag
    22981 ggccagggct ggatcaccgc agagtacggc atgctgccac gttctaccca cacccgtaac
    23041 gctcgtgaag cggcgaaagg taagcagggt ggacgcacaa tggaaatcca gcgtctgatc
    23101 gcccgtgctc ttcgcgcggc agtagatttg aaagcgctgg gtgagttcac cattacgctg
    23161 gactgcgacg tgcttcaggc tgatggtggc acgcgtaccg cgtcgattac gggtgcctgc
    23221 gtggcgctgg cagatgcgct acagaagctg gtggaaaacg gcaagctgaa aaccaatccg
    23281 atgaaaggga tggtagccgc agtttctgtc ggaattgtga acggcgaagc ggtttgcgat
    23341 ctggaatacg ttgaagactc tgccgcagag accgacatga acgtagtgat gaccgaagac
    23401 gggcgcatca ttgaagtgca ggggacggca gaaggcgagc cgttcaccca tgaagagcta
    23461 ctcaccttgt tggctctggc ccgaggggga atcgaatcca ttgtagcgac gcagaaggcg
    23521 gcgctggcaa actgattttt aaggcgactg atgagtcgcc ttttttttgt ctgtagaaaa
    23581 gtaagatgag gagcgaaggc atgaaaccat atcagcgcca gtttattgaa tttgcgcttg
    23641 gcaagcaggt gttaaagttt ggcgagttta cgttgaagtc cgggcgcaaa agcccctatt
    23701 tcttcaacgc cgggctgttt aataccgggc gcgatctggc actgttaggc cgtttttacg
    23761 ctgaagcgtt ggtggattcc ggccttgagt tcgatctgct gtttggccct gcttacaaag
    23821 ggatcccgat tgccaccact accgccgtgg cgctggcgga gcatcatgac cttgacctgc
    23881 cgtactgctt taaccgcaaa gaggcaaaag accacggtga aggcggcaat ctggttggta
    23941 gcgcgttaca aggacgcgta atgctggtag atgatgtgat caccgccgga acggcgattc
    24001 gcgaatcgat ggagattatt caggctaatg gcgcgacgct tgctggcgtg ttgatttcgc
    24061 ttgatcgtca ggaacggggg cgcggcgaga tttcggcgat tcaggaagtt gagcgtgatt
    24121 acaactgcaa agtgatctct atcatcaccc tgaaagacct gattgcttac ctggaagaga
    24181 agccggaaat ggcggaacat ctggcggcgg ttaaggccta tcgcgaagag tttggcgttt
    24241 aaagaaactc gccggatgaa aagtcatccg gcgtcatatt actgcaactg tgccgcaatt
    24301 agcggccagc gggcgtcaaa atcatccgtc gggcggtatt taaattcgct gcggacaaaa
    24361 cgtgacagca taccttcaca gaaggccagg atctggcttg ccagcagggt ttcatcggtg
    24421 gtgtaacctt caccctcacg cattctcttt tcacgcaata cctgacgcag ctgcgcttca
    24481 atacgctcga acagctggtt gatgcgccct tgcaggcgat cctgttcaaa cattagcgca
    24541 tgaccagtga ggatgcgggt caggccagga ttacgctcac caaaaccgag aagcagcaac
    24601 acaatcagac gcaggcgcgc tgtggtgtct ttctcatctt tcagaatcag attgatgcga
    24661 gtaatcaggc tatcttcgat aaactcaatc aggctatcga acatgcgggt cttactgggg
    24721 aagtggcgat acagtgccgc ttcggaaacg ccgacagagg cggccagttt tgccgtcgtg
    24781 atacgttggc ttccatcgct ggattccagc atcagcgcca gagactgaag tatttcctcg
    24841 cgacggttcc ttttcgcagt ttgtttttct gccatgttac aaaatacccc tgaaaataag
    24901 cacttgccag gcggcaccca cgctatgacc gcaaacgaaa tgtttgcggc tatgttatga
    24961 cgttattcgg atgcgtatgt gttactgacg accagagtga ccaaagccgc cttcaccgcg
    25021 gtcggtggcg tcgaaatctt ccaccagatt aaattcagcc tgtactaccg gaacaaaaat
    25081 catctgggcg atgcgttcgc caggttgaat ggtgaagctg tcctgaccac ggttccacac
    25141 ggaaatcatc aactggccct gatagtcgga atcgatcaat cctaccaggt taccaagcac
    25201 gataccgtgc ttatgtccca atccggagcg cggcagcatc attgccgcca gtgaaggatc
    25261 ggcaatatga atcgccagcc cggtcggaac cagcgtagtg tcacccggag ccagttctac
    25321 ggcgtcgtcg agacaggcac gcaggtcaag tccggcagag ccagaggtgg cataagtcgg
    25381 gagcggaaat tccttcccaa cgcgcgggtc cagaatctta acgtcgattt ttttcatcat
    25441 aacgggtcac gatctcgtcg agtaataatt ggccaaggag ctctttgcgc tcaagcggta
    25501 agactttatc tccgtcctgc cagaaaaggt gtaatgcgtt gttgtcgctg ttaaatcctt
    25561 gagttggctg ggaaacatcg ttcgcgcaga tcagatcaag gtttttacgg atacgttttt
    25621 gccgggcgta ttcttccaca ttatttgttt cggcggcaaa tccaacgacg tagggtcgat
    25681 ggtcttttag tgcggcaacg cctgcgacga tatcggggtt tttaaccatt tttattgtta
    25741 attcatcacc ctgcgtggcc tgctttttga ttttctctgg ggccacggta gctgcgcgat
    25801 aatccgccac ggcggcgcag ccgataaaaa tattttgctg ctgtacagaa gcattcacgg
    25861 cggcttccat ttccagcgcg gtcatcacat caacacgttt aacaaacggt ggcgtcggta
    25921 gtgaaaccgg acctgatacc agcgtgacgt tcgcgccacg acgggcagcg gcggcggcga
    25981 tagcaaaacc catcttgccg gagctgtgat tagagatata acgcacggga tcgagcggtt
    26041 cacgcgtcgg gccggcggta atcataatgt tcagatgttt caggtcgttg acgggcgaaa
    26101 aatgcgctac cgccatatcc acaatggtta acggatcgag cattcgccca ggaccgatat
    26161 caccacaagc ctgactgcca ctgtctggcc cccagatgag caaaccacgg gaagcaagca
    26221 cctctaaatt atgctgcgtg gcagcggcac ggtacatctg ctggttcatg gcggggagca
    26281 cggctacagg cgcaggtgta gccagacaaa tcgtcgatac caggtcattc gccattccgg
    26341 cagcaacacg ggcaatcaaa tctgccgtgg caggggcgag aatcactaaa tcagcccatt
    26401 tacccagctc aatatggccc atagcggctt cggctgccgg gtccagcaga ctgtcggaaa
    26461 cgggataacc agaaaccgcc tgcaagctaa gtggggtgat aaaggctttt gccgcttcgg
    26521 tcatggctac gcggacgtcg gccccgcgat cgcgcaaacg acgcaccagt tcaggggttt
    26581 tataggcagc aataccgccg ctaacgccga gaacgatttt tttaccggcc aggctcatga
    26641 tgatttttcc tgttgggtga caccagaagt tggcgatttt atcacaatac ttttgttgtc
    26701 gtgccttcac ctgagaatca ctttgcgagg cgctttccag gattgaaaac tggccgtcga
    26761 tttaacggaa cggctatgac aggatgcgag caccacaaag gaggtgaagg tgaaaaacaa
    26821 ttcacagctg ttgatgccgc gcgaaaaaat gctgaagttt ggtattagcg ccttaacgga
    26881 tgtcgagctg ctggcgctat ttctgcgtac cggaacgcgc ggtaaagatg tattaaccct
    26941 ggcaaaagag atgctggaga atttcggctc tctttatggc ttgttaacct ctgaatatga
    27001 acaatttagt ggcgttcatg gaattggcgt ggcgaaattt gcccagttaa aggggattgc
    27061 tgaactggcg cggcgttact acaacgtgcg gatgcgtgaa gaaagccctt tactcagccc
    27121 ggagatgacg agggaatttt tacaaagcca gctcacgggt gaggagcggg agatctttat
    27181 ggtgatcttt ctcgactccc aacaccgggt tataacgcat agccgtcttt tttccggcac
    27241 gctaaaccat gttgaagtcc atcctcggga aattatccgc gaagcgataa aaataaacgc
    27301 ctcggcgctg atccttgcac ataatcaccc ttcgggttgt gctgaaccca gtaaagcgga
    27361 taaactcatt actgaacgga taataaagag ttgtcagttc atggatttac gcgtgctcga
    27421 ccatatcgtg attgggcgtg gagagtatgt ttcttttgcc gaacgcggct ggatttaacc
    27481 cgctatgcgc gatccttcgg gatctttgtc tgttcgggac ttgagcacat cgctgagtca
    27541 gcgtatacta cgccaccttt gagaatctcg ggtttggcat ttgggcctgg caatcgagag
    27601 ttcacataga actgcgatga ccgggctgta aagcctgacg aggcgccaat accccatacg
    27661 aagctcgagc taatttgatt tttggagaat agacatgtcc cgagtctgcc aagttactgg
    27721 caagcgtccg gtgaccggta acaaccgttc ccacgcactg aacgcgacta aacgccgttt
    27781 cctgccgaac ctgcactctc accgtttctg ggttgagagc gagaagcgtt ttgtcaccct
    27841 gcgcgtatct gctaaaggta tgcgtgtaat cgataaaaaa ggcatcgata cagttctggc
    27901 tgaactgcgt gcccgtggcg aaaagtacta agtacttaga ggaaataaat catggctaaa
    27961 ggtattcgtg agaaaatcaa gctggtttct tctgctggta ctggtcactt ctataccact
    28021 acgaagaaca aacgtactaa gccggaaaaa ctggaactga aaaaattcga tccagttgtt
    28081 cgtcagcacg tgatctacaa agaagcgaaa atcaaataat tctcgctttg atgtaacaaa
    28141 aaacctcgct ccggcggggt tttttgttat ctgcttgccc ccatattgat tgcatctgtt
    28201 cattcctgga gatgctatgc ctgaattacc cgaagttgaa accagccgcc gcggcataga
    28261 accgcatctc gttggtgcaa ccattcttca tgcagtggtg cgcaacggac gcttgcgctg
    28321 gccggtttca gaagagatct accgtttaag cgaccaacca gtgcttagcg tgcagcggcg
    28381 ggctaaatat ctgctgctgg agctgcctga gggctggatt atcattcatt tagggatgtc
    28441 tggcagcctg cgcatccttc cagaagaact tccccctgaa aagcatgacc atgtggattt
    28501 ggtgatgagc aacggcaaag tgctgcgcta caccgatccg cgccgctttg gtgcctggct
    28561 gtggaccaaa gagctagaag ggcacaacgt gctggctcat cttggaccgg agccgcttag
    28621 cgacgatttc aatggtgagt atctgcatca gaagtgcgcg aagaaaaaaa cggcgattaa
    28681 accgtggctg atggataaca agctggtggt aggggtaggg aatatctatg ccagcgaatc
    28741 actgtttgcg gcggggatcc atccggatcg gctggcgtca tcactgtcgc tggcagagtg
    28801 tgaattgtta gctcgggtga ttaaagcggt gttgctgcgt tcgattgagc agggtggtac
    28861 aacgctgaaa gattttctgc aaagtgatgg taaaccgggc tatttcgctc aggaattgca
    28921 ggtttacggg cgaaaaggcg agccgtgtcg ggtgtgcggt acgccgattg tggtgactaa
    28981 acatgcgcag cgggcaacgt tttattgtcg gcagtgccag aagtaattca tgcgcgccgg
    29041 atggcatacc atccggcata aacgctacgc taacttcgcc atcagcgcct gatggacatt
    29101 ctccggcagg aaatgggtga catcgccctg atggcgcgcc acctctttca ccaacgatga
    29161 agagataaac gaccactctt tcgacggcat cagaaacaca ctttccagtt ccggcattaa
    29221 gtggcgattc atatgcgcca gctgcatttc atattcaaaa tccgccaccg cacgcaggcc
    29281 acgaatcagc accgtagcgt gttgattacg ggcgaagttc gccatcaaat cactaaaccc
    29341 gaccacttcc acgttcccca gatgcgcggt tgcctgctgt gccagcgcca cacgctcttc
    29401 cagggtaaac atcggttttt tactggggct ggcggcaatc gccagaataa cgtgatcgaa
    29461 catttgcgtg gcgcgcgtca cgatatcgat atgaccattg gtaatgggat cgaaagtacc
    29521 cggataaatc gcccgttttt gcataacaac ctcaatgcgt tttcggtggc aggtaaggtt
    29581 ccagcagttg aagcagacgc tgtagcgcgc cctggttttg atacagtact tcaacggcat
    29641 gacggccata gaaactacgg taatcggcgt cggtgagtaa agaggacacc tcttttgcaa
    29701 gcgtagtggc atcggtaacg gtaatcagcc cgcttgcctg ctccagccgc gcgcaaatgt
    29761 ctttaaagtt aaaagtatgc ggccccatca ataccggaat agcgtgtgcg gcagcttcca
    29821 gcggattatg cccaccacgt tcaaccagtg aaccgccaac aaaggcgaga tcggcaatgc
    29881 cgtacagtaa catcaactcg cccatcgtat cgccaactac aacctgcgtg ctggtggagg
    29941 ggacttcccc tgaagagcgt gtgatatagc ttagtccagc ctggcggaca agattaatcg
    30001 catccgggaa gcgttctgga tgacggggta ccaggatgag caataaattc gggaattgct
    30061 gtaacaatgc ctgatgtgcg gcgatcacca cactctcttc gccttcgtga gtgctggtgg
    30121 caatccatac cgggcggtgt ggtgcccact ggcggcgcag cgtcacggct ttagcagcca
    30181 actgcggcgt tacagaaata tcgaatttca ggctaccggt aacggtcacc tgattatttt
    30241 ttgcgcccag cgccacaaaa cgtgcaccat cttcttcatt ttgcgcagca atcagcgtaa
    30301 tacgacgcag caagcggcgg acgaatttac ccagtttggc ataacctgcg gccgagcggg
    30361 cagagagtcg cgcgttagcg atcaccagcg gaattttacg tttatgtagc gccgcaatca
    30421 ggttaggcca tagttcggtt tccataatca acaccagttt ggggtcgact ttattcagga
    30481 aacggttgag cgcatcgggc aggtcatacg gcagataaac gtgctgaaca tccttcccga
    30541 aagccgattg tacgcgctcc gaaccggttg gcgtcatggt tgtaacggta atcggtaaat
    30601 caggataacg atgacgcagc gcgcgcacca acgggatcgc cgccagagtt tcaccgacgg
    30661 agacggagtg cagcataatg ccgcctggtt ttagcggatg gcggtaaaaa ccgtaacgtt
    30721 caccccagcg ttttcgatag gccggagcct tacgtccgcg cacccagagc cgtatccaga
    30781 tcagcggctg aataaggtag agaagggcgg tgtaaagcaa ttcgagcata gtaaatagct
    30841 gacttatgga tgtgctgggg attctatgta tttagctgtg gctttaccat tacttttccc
    30901 gtttttgact taaatagcaa tttacccgcc aattcttgct gaacatctat actttttttg
    30961 tatttttatg cgattcattg atttctgctg tacgtgcagg aaggaatcgc agagcaacag
    31021 tgaaagaata tggttgctaa tgattttatt tgattgattt ctttattgaa aatcaacaat
    31081 gtgcttaaaa atcattcggg tcggattgcc gcgaaagaag tgatacacta gcgcgtcaat
    31141 atcagtgcgg tatcagtatt caggtagctg ttgagcctgg ggcggtagcg tgctttttct
    31201 gcttcacctt aatcggataa tctcaacaaa aagagttact tgtggataag ccatttcgaa
    31261 gaattttgct cattaagatg cgttttcatg gggatatgtt attaactact cccgtcatta
    31321 gttcgctgaa aaaaaattac cctgacgcaa aaatcgatgt gctgctttat caggacacca
    31381 tcccgatcct gtctgaaaat ccagagatta acgcgctcta cggcataaaa aataaaaaag
    31441 caaaagcctc agaaaaaatt gccaactttt ttcatctcat caaggtatta cgtgccaata
    31501 agtatgacct tatcgtcaat ctcaccgatc aatggatggt tgctatactg gttcgcttat
    31561 taaatgcccg tgtgaaaatt tcccaggatt atcatcatcg gcagtctgct ttttggcgta
    31621 aaagtttcac ccatttggtg ccgttgcagg gtggaaatgt ggtggaaagt aacttatccg
    31681 tgctgacccc attgggagtt gattcgttgg tgaagcagac aaccatgagt tacccgcctg
    31741 caagctggaa acgtatgcgt cgcgaacttg atcacgctgg tgttggacaa aattatgtgg
    31801 ttatccaacc tacggcgcgg caaatcttca aatgctggga caacgccaag ttttccgctg
    31861 tgattgatgc cttacatgct cgtggttatg aagttgttct gacgtccggc ccagataaag
    31921 acgatctggc ctgcgtcaat gaaattgcgc agggatgcca gacgccacca gtaacggcgc
    31981 tggctggaaa ggtgaccttc ccggaacttg gtgcgttaat cgatcatgcg cagctgttta
    32041 ttggcgttga ttccgcaccg gcgcatattg ccgctgcagt taatacgccg ctgatatcgc
    32101 tgtttggtgc gacagatcat attttctggc gtccctggtc aaataacatg attcaattct
    32161 gggcgggaga ttaccgggaa atgccaacgc gcgatcagcg tgaccgaaat gagatgtatc
    32221 tttcggttat tccggcggca gatgtcattg ctgctgtcga taaattactg ccctcctcca
    32281 cgacaggtac gtcgttatga tcgttgcttt ttgtttatat aaatattttc cctttggcgg
    32341 tttgcagcgt gattttatgc gtattgctca gacagtcgcc gcccgaggtc atcatgttcg
    32401 ggtttatacc cagtcgtggg aaggcgaatg ccctgatgta tttgaactga tcaaagtgcc
    32461 ggttaaatcg cataccaatc acgggcgcaa tgcggagtat tttgcctggg tgcaaaaaca
    32521 tttacgcgaa catcccgtcg ataaagtcgt tggattcaac aaaatgccgg ggctggacgt
    32581 ttattatgcc gctgatgttt gttatgccga gaaagtagcg caggaaaaag gctttttcta
    32641 tcgcctgacg tcacgttatc gccattatgc cgcctttgag cgggcaacct tcgaacaggg
    32701 taagccgaca cagttgctga tgctgacaga taagcaaatc gccgatttcc agaaacatta
    32761 tcagactgaa gcggagcgtt ttcatattct gccaccgggg atttatcctg atcgtaaata
    32821 tagccagcag ccagccaata gccgtgaaat cttccgtaag aagaatggaa taaccgaaca
    32881 acaatattta ttgttgcagg tcggttcaga cttcacgcgt aaaggtgtcg atcgttccat
    32941 tgaagcactt gcttcgttac cggattcgct gcgccacaac acattgctat atgttgttgg
    33001 gcaggataaa ccgcgaaaat ttgaggcgct ggcagaaaaa cgcggcgtgc gcagtaatgt
    33061 tcactttttc tcggggcgca atgatgtctc ggaattaatg gcggcggcgg atttattact
    33121 gcatcctgcc taccaggaag cggcgggaat tgtgctgctg gaagcgatta ctgcaggatt
    33181 accggtacta acaacagccg tttgtggcta tgcgcattat attgtcgacg ctaattgcgg
    33241 cgaggctatt gctgagccat tccgccagga aacattgaat gagattttac gcaaagcgtt
    33301 aacgcaatct tcattgcgcc aggcctgggc ggaaaatgcg cgacattatg ctgatacaca
    33361 agatttatac agtctgccag agaaagcggc ggatatcata acgggtggtc tggatggttg
    33421 aacttaaaga gccgtttgcc acgttatggc gcggcaaaga tccttttgag gaagttaaaa
    33481 ccttgcaggg tgaggtattt cgtgaactgg aaactcgccg tactctgcgc tttgaaatgg
    33541 cgggcaaaag ctattttctc aaatggcatc gcggcacgac cctgaaagag ataatcaaaa
    33601 atttactctc attgcggatg ccagtattag gcgctgaccg cgaatggaat gcgattcatc
    33661 gactgcggga tgtcggcgtt gatactatgt atggggtggc atttggcgaa aaaggcatga
    33721 atccgctgac cagaacttca tttattatta ccgaagatct gacaccaacc ataagtcttg
    33781 aagattactg tgctgactgg gcgactaacc ctccagatgt tcgcgtaaag cgtatgctta
    33841 ttaagcgtgt cgcgacgatg gtgcgcgata tgcatgctgc gggcattaac caccgtgact
    33901 gttatatctg tcatttcctg ctgcacttgc ctttttccgg taaggaagag gagttaaaaa
    33961 tttcggtaat tgacctgcac cgggcgcagc ttcgcacgcg cgttccacgt cgttggcggg
    34021 ataaagatct tattgggctt tatttttctt cgatgaatat cggcctgact cagcgggata
    34081 tctggcggtt tatgaaagtg tattttgccg ccccgcttaa agacattctc aagcaggaac
    34141 aaggactgct gtcgcaagca gaagcaaaag ccacaaaaat cagggaaaga acgattcgaa
    34201 aatcgttgta acccgtaact tatttttgcc aaaattttgg atacagaata aatatgtctc
    34261 aactcaatga tagtgacatc atcctttttg agtataattt tcattatc
//
LOCUS       DAZFRN010000038        33489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFRN010000038 DAZFRN010000000
VERSION     DAZFRN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.038"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..581
                     /gene="yraH"
                     /locus_tag="ACHKUB_001781"
     CDS             <1..581
                     /gene="yraH"
                     /locus_tag="ACHKUB_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6654808.1"
                     /translation="KVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSDKK
                     IEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRDNG
                     TVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGIVN
                     VDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            661..1356
                     /gene="yraI"
                     /locus_tag="ACHKUB_001782"
     CDS             661..1356
                     /gene="yraI"
                     /locus_tag="ACHKUB_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6654809.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1386..3902
                     /gene="yraJ"
                     /locus_tag="ACHKUB_001783"
     CDS             1386..3902
                     /gene="yraJ"
                     /locus_tag="ACHKUB_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6654810.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3913..5004
                     /gene="yraK"
                     /locus_tag="ACHKUB_001784"
     CDS             3913..5004
                     /gene="yraK"
                     /locus_tag="ACHKUB_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6654811.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQTNDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFNITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5047..5907)
                     /gene="rsmI"
                     /locus_tag="ACHKUB_001785"
     CDS             complement(5047..5907)
                     /gene="rsmI"
                     /locus_tag="ACHKUB_001785"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO6654812.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5971..8007
                     /gene="lpoA"
                     /locus_tag="ACHKUB_001786"
     CDS             5971..8007
                     /gene="lpoA"
                     /locus_tag="ACHKUB_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO6654813.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7965..8360
                     /gene="yraN"
                     /locus_tag="ACHKUB_001787"
     CDS             7965..8360
                     /gene="yraN"
                     /locus_tag="ACHKUB_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO6654814.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWHARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8380..8970
                     /gene="diaA"
                     /locus_tag="ACHKUB_001788"
     CDS             8380..8970
                     /gene="diaA"
                     /locus_tag="ACHKUB_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO6654815.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8980..9555
                     /gene="dolP"
                     /locus_tag="ACHKUB_001789"
     CDS             8980..9555
                     /gene="dolP"
                     /locus_tag="ACHKUB_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO6654816.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9669..10709)
                     /gene="yraQ"
                     /locus_tag="ACHKUB_001790"
     CDS             complement(9669..10709)
                     /gene="yraQ"
                     /locus_tag="ACHKUB_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO6654817.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10782..11417)
                     /gene="yraR"
                     /locus_tag="ACHKUB_001791"
     CDS             complement(10782..11417)
                     /gene="yraR"
                     /locus_tag="ACHKUB_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO6654818.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11545..12063
                     /gene="yhbO"
                     /locus_tag="ACHKUB_001792"
     CDS             11545..12063
                     /gene="yhbO"
                     /locus_tag="ACHKUB_001792"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO6654819.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12043..12486)
                     /gene="yhbP"
                     /locus_tag="ACHKUB_001793"
     CDS             complement(12043..12486)
                     /gene="yhbP"
                     /locus_tag="ACHKUB_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO6654820.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12537..12839
                     /gene="yhbQ"
                     /locus_tag="ACHKUB_001794"
     CDS             12537..12839
                     /gene="yhbQ"
                     /locus_tag="ACHKUB_001794"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO6654821.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12826..13329)
                     /gene="yhbS"
                     /locus_tag="ACHKUB_001795"
     CDS             complement(12826..13329)
                     /gene="yhbS"
                     /locus_tag="ACHKUB_001795"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6654822.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13323..13847)
                     /gene="ubiT"
                     /locus_tag="ACHKUB_001796"
     CDS             complement(13323..13847)
                     /gene="ubiT"
                     /locus_tag="ACHKUB_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO6654823.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14056..15051
                     /gene="ubiU"
                     /locus_tag="ACHKUB_001797"
     CDS             14056..15051
                     /gene="ubiU"
                     /locus_tag="ACHKUB_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO6654824.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15060..15938
                     /gene="ubiV"
                     /locus_tag="ACHKUB_001798"
     CDS             15060..15938
                     /gene="ubiV"
                     /locus_tag="ACHKUB_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO6654825.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            16144..17151
                     /gene="yhbW"
                     /locus_tag="ACHKUB_001799"
     CDS             16144..17151
                     /gene="yhbW"
                     /locus_tag="ACHKUB_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO6654826.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17269..18513)
                     /gene="mtr"
                     /locus_tag="ACHKUB_001800"
     CDS             complement(17269..18513)
                     /gene="mtr"
                     /locus_tag="ACHKUB_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO6654827.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18667..20556)
                     /gene="deaD"
                     /locus_tag="ACHKUB_001801"
     CDS             complement(18667..20556)
                     /gene="deaD"
                     /locus_tag="ACHKUB_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO6654828.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20549..20629)
                     /gene="yrbN"
                     /locus_tag="ACHKUB_001802"
     CDS             complement(20549..20629)
                     /gene="yrbN"
                     /locus_tag="ACHKUB_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO6654829.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20736..21620)
                     /gene="nlpI"
                     /locus_tag="ACHKUB_001803"
     CDS             complement(20736..21620)
                     /gene="nlpI"
                     /locus_tag="ACHKUB_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO6654830.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21729..23864)
                     /gene="pnp"
                     /locus_tag="ACHKUB_001804"
     CDS             complement(21729..23864)
                     /gene="pnp"
                     /locus_tag="ACHKUB_001804"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO6654831.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24111..24380)
                     /gene="rpsO"
                     /locus_tag="ACHKUB_001805"
     CDS             complement(24111..24380)
                     /gene="rpsO"
                     /locus_tag="ACHKUB_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO6654832.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24529..25473)
                     /gene="truB"
                     /locus_tag="ACHKUB_001806"
     CDS             complement(24529..25473)
                     /gene="truB"
                     /locus_tag="ACHKUB_001806"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO6654833.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25473..25874)
                     /gene="rbfA"
                     /locus_tag="ACHKUB_001807"
     CDS             complement(25473..25874)
                     /gene="rbfA"
                     /locus_tag="ACHKUB_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO6654834.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26038..28710)
                     /gene="infB"
                     /locus_tag="ACHKUB_001808"
     CDS             complement(26038..28710)
                     /gene="infB"
                     /locus_tag="ACHKUB_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO6654835.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28735..30222)
                     /gene="nusA"
                     /locus_tag="ACHKUB_001809"
     CDS             complement(28735..30222)
                     /gene="nusA"
                     /locus_tag="ACHKUB_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO6654836.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30250..30702)
                     /gene="rimP"
                     /locus_tag="ACHKUB_001810"
     CDS             complement(30250..30702)
                     /gene="rimP"
                     /locus_tag="ACHKUB_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO6654837.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30909..30985)
                     /locus_tag="ACHKUB_001811"
     tRNA            complement(30909..30985)
                     /locus_tag="ACHKUB_001811"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30949..30951),aa:Met,seq:cat)
     gene            31333..32676
                     /gene="argG"
                     /locus_tag="ACHKUB_001812"
     CDS             31333..32676
                     /gene="argG"
                     /locus_tag="ACHKUB_001812"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO6654838.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKVK"
     gene            32860..>33489
                     /locus_tag="ACHKUB_001813"
     CDS             32860..>33489
                     /locus_tag="ACHKUB_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6654839.1"
                     /translation="MNGEYFVRLALHTLKCTQKDLASHLGVSSTQISKWKKGEHMSAD
                     MEKKFRNITQIGDYSPQLVEWTGSVANAEKWDRLIHFLARRAMDDAETGYITQLLTDE
                     DGFLVEETIDVLKRIGFPTPLSFPEGLNIDDDNADEEEAFWEILESNAHCSVINDIYH
                     ALNDVYGFYIAYVDELIQDDDLDVYSSEAINIQSSLISLAACKIEIDTPV"
BASE COUNT         8242 a         8968 c         8303 g         7976 t
ORIGIN      
        1 ataaagttac aaaaacagct attgtgggct tacttgcgct tttcgcaggt aatgccgctg
       61 caaccgatgg tgaaatagtt tttgatggcg agattttaaa atctgcttgt gaaatcaatg
      121 actctgataa gaaaattgaa gttgctcttg gtcactataa tgccgaacag tttcgttctg
      181 ttggcgatcg tagcccaaaa attccattca ctattccttt agttaactgc ccagtgactg
      241 ggtgggagca cgataacggc aacgttgaag cgtctttccg tttatggctg gaaacgcggg
      301 ataatggcac cgtccctaac ttccctaatc tggcaaaagt gggctctttt gctggcacag
      361 cggcaacagg tgtgggtatc cgtattgacg atgcggaaag cggaaacctt atgccactga
      421 atgctatggg gaatgataac acggtttatc agatcccagc ggactccaat ggtattgtca
      481 atgttgacct catcgcttac tacgtatcaa ccgttgaagc atcagaaatc accccagggg
      541 aagcagacgc tgtcgttaac gtaacgctgg attaccgtta atacgttacg gcgttatctg
      601 acctttcaga taacgccctt ttccttcctc tttctcgttg tatcaggttg aaaaataact
      661 atgtcaaaac gaacattcgc ggtgataata accttgttgt gtagcttctg tatcggccag
      721 gcgcttgcag ggggtatcgt tttacagcga acgcgagtga tctatgatgc cagccgcaaa
      781 gaggctgcgt tacctgtcgc aaacaaaggc gcagaaacgc cttatttact gcaatcatgg
      841 gtagataata tagatggtac aagccgtgcc ccatttatta taaccccacc gctatttcgt
      901 cttgaagctg gcgatgactc atcactgcga attattaaaa cagctgataa cctgcctgaa
      961 aataaagagt cgctgttcta cattaatgtt cgtgccattc cagcaaagaa aaaatcagat
     1021 gatgttaatg ctaacgagtt gacgctggta tttaaaacac ggatcaaaat gttttatcgc
     1081 cccgcacacc tgaagggacg ggtaaacgat gcgtggacat cactggaatt taaacgtagt
     1141 gaccattcac tcaatatata taacccaact gaatattacg tcgtatttgc cggactggca
     1201 gtcgataaaa ccgatctcac aagcaaaatt gaatatatcg cgcccggaga acataaacag
     1261 ttaccacttc ctgcatctgg cggaaagaac gtgaaatggg ctgcaatcaa tgattatggc
     1321 ggcagttccg ggacagaaac tcgtccactg caataacaaa tataaaaaac acaggtcatc
     1381 agggaatgcc acaacgacac caccagggac ataaacgcac accgaaacag ttggcgctca
     1441 tcatcaaacg ctgtttgccg atggtgctca ctggcagcgg catgctttgc actaccgcta
     1501 acgccgaaga gtattatttc gaccccatta tgctggaaac tacaaaaagt ggtatgcaaa
     1561 caactgatct gtcacgtttt tcaaaaaaat acgcacaact accaggaact tatcaggttg
     1621 atatctggct gaataaaaag aaggtttcac agaaaaaaat tacatttacc gccaatgcag
     1681 agcaacttct gcagccacag tttacggtag aacaactacg tgagctgggt attaaggtgg
     1741 atgaaatccc ggcgctggct gaaaaagatg acgatagcgt gatcaactcg cttgaacaaa
     1801 tcattcccgg tacagctgct gaatttgatt tcaatcatca gcgacttaat ttgagcattc
     1861 cccaaattgc actgtaccgt gatgcaagag gttacgtctc cccttctcgt tgggacgatg
     1921 gtataccaac gctgtttacc aactactcgt ttacaggttc tgataaccgt taccgccagg
     1981 gcaatcgtag ccaacgacag tacctgaata tgcaaaatgg tgctaatttt ggcccctggc
     2041 gattacgtaa ctattctacg tggacacgca acgatcaggc gtcaagctgg aacactatca
     2101 gtagttattt acaacgtgat atcaaggcgt tgaagtctca gttgcttctg ggagaaagcg
     2161 ccaccagcgg cagtattttt tccagctaca cctttactgg cgtgcaactc gcttccgacg
     2221 ataatatgtt gccaaacagc cagcgcggat ttgccccaac ggtacgcggt atcgcaaaca
     2281 gtagtgcaat cgtgactatc aggcaaaatg gttatgtgat ctatcaaagc aacgtgccag
     2341 cgggtgcctt tgaaattaac gatctctacc cctcttccaa cagcggcgat ttagaagtca
     2401 cgattgaaga aagtgacggt acgcaacgtc gctttatcca gccttattct tcattaccca
     2461 tgatgcagcg acctgggcat ctaaaatata gcgcgaccgc tggacgctat cgcgctgatg
     2521 caaacagtga tagcaaggaa cccgaatttg ctgaagccac ggcaatatat ggtttgaata
     2581 atacttttac gctgtatggc ggcctgctcg gttctgaaga ttattatgcg ctggggatcg
     2641 gtatcggcgg cacacttggc gcactgggcg cgttgtcgat ggatatcaac agagctgaca
     2701 cccaattcga taaccagcac tcttttcatg gctatcaatg gcgtacgcag tacatcaaag
     2761 atatcccgga aaccaacacc aatatcgctg ttagctacta tcgctatacc aacgatggct
     2821 attttagttt tgatgaagcc aatacccgta attgggacta taacagtcgc caaaaaagtg
     2881 aaattcaatt caacatcagc cagacaatat ttgatggggt aagtctgtat gcctccggtt
     2941 cgcagcaaga ctattggggc aataacgata aaaacaggaa tatctcagtt ggggtttccg
     3001 gtcagcaatg gggaattggt tacagcctga attatcaata cagtcgctac acggatcaaa
     3061 ataatgaccg cgcactctct ttgaatctca gtattccgtt agaacgctgg ttaccgcgta
     3121 gccgggtttc ctatcagatg accagccaga aagatcgccc aacccaacat gaaatgcgtc
     3181 ttgatggctc actgctggat gatggtcgcc tgagctatag cctggaacaa agtctggatg
     3241 acgataacaa ccataacagt agcctgaacg ccagttaccg ttcaccttat ggcaccttca
     3301 gtgccggata cagctacggt aatgacagca gccaatacaa ttacggcgtt accggcggcg
     3361 tggttatcca tcctcatggc gtgacgctct cgcaatatct gggcaacgct tttgcgctta
     3421 tcgatgctaa tggggcttct ggcgtgagga tacaaaacta tccggggatt gctactgatc
     3481 cctttggcta tgcagtggtt ccttatctca cgacttacca ggaaaaccgt ctctcggtag
     3541 atactacgca gctgcccgat aacgtcgatc ttgagcaaac aacacagttt gtggtgccca
     3601 acagaggtgc aatggtagcg gcgcgtttca acgccaatat cggttatcgc gtacttgtta
     3661 cagtcagcga tcgcaacggt aaaccgttgc cctttggcgc tcttgccagc aacgatgaga
     3721 cggggcaaca aagtatcgtc gatgagggcg gcatactata tctctctggg atatcgagta
     3781 aatcacaaag ctggactgta cgctggggaa atcaggcaga tcaacaatgt cagtttgctt
     3841 ttagtacacc ggattcagaa ccaacaacct ctgtattaca aggcacggcg cagtgccatt
     3901 aaggataaaa aaatgaaaag agcgcctctt ataacaggac ttttgttgat atccacatcc
     3961 tgcgcttatg cctcctcagg agggtgtgga gctgacagca ctagcggtgc gacaaattac
     4021 agcagtgtgg ttgatgatgt tacggtgaac cagacagata acgtgacagg acgggagttt
     4081 acctctgcca cgctaagtag cactaactgg caatacgcct gtacctgctc tgcgggtaag
     4141 gcagttaaac ttgtctatat ggtcagccct gtacttacca ccactggaca tcaagcagga
     4201 tattacaaac tcaatgatag tctggatatt aaaaccacat tacagacgaa tgacattcca
     4261 ggactcgtga ctgaccagac cgtttctgtt aacacccgat tcacacagat aaaaagcaac
     4321 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
     4381 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
     4441 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcatgggt cgatggaatg
     4501 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
     4561 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
     4621 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
     4681 tttaatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
     4741 atcgacggta caactggggt agtagaccag tacaacctgg tcgccagacg aagaagttca
     4801 gacaatgcgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
     4861 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccat caacatgcgc
     4921 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
     4981 accattaccg tcatcgtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
     5041 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccgtg
     5101 aatttctgcc gccagcgccg ccgctttttt caacggcagt tctgcctgta gcagcgccag
     5161 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgataatcag
     5221 caccatttcg cctttgcgac ggttttcatc ttcttttacc cacgccagca gctcgccaac
     5281 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     5341 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtggattc
     5401 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     5461 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     5521 taacgcagtg atagcagcac acggcccggg tagcggcacc acgcaaatcc ccgcttcacg
     5581 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     5641 cagcgcaatg ttctgccctt cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     5701 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     5761 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     5821 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     5881 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     5941 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     6001 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     6061 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     6121 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     6181 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     6241 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     6301 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     6361 gaacaaaacc agcaagcccg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     6421 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaag
     6481 cagcagaata ttgacgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     6541 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     6601 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     6661 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     6721 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cactgaatgg ccaggcagcg
     6781 gtatttggtc gcactattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     6841 gtggcagctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     6901 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgaacag
     6961 cctgcagccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     7021 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     7081 atcttaagcc aggttcagca ggatggcgcg agtattgtgg tcggtccgtt gctgaaaaat
     7141 aacgttgaag agttgctgaa gagcaacact ccgctgaacg tactggcact gaaccagccg
     7201 gagaatatcg aaaatcgcgt caatatttgt tacttcgcgc tttcaccgga agacgaagcg
     7261 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     7321 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactaggc
     7381 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     7441 ggcggttctg gtattgcttt aacgggtagc ccgattactc ccagagcgac aaccgactcc
     7501 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     7561 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     7621 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caacgctgta cgccagctcc
     7681 cgcagtgcgc aagggaccgc tggcccggat ttccgactgg agatggaagg cttgcagtac
     7741 agcgaaatcc cgatgctggc aggcggtaat ctaccgttaa tgcagcaggc actcagcgcg
     7801 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     7861 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     7921 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     7981 caaggtcagg tagtccccgc cagttaacca ccaaacagac cggcgatgcg tgggaagtac
     8041 aagcgcgtcg ctggctggaa ggcaaaggac tgcgatttgt cgccgctaac gtgaacgagc
     8101 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     8161 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     8221 acaaattatt acagactgcc cgcttgtggc acgcgcgtca taatgggagt tttgatactg
     8281 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     8341 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     8401 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     8461 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     8521 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     8581 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     8641 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     8701 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     8761 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg atatgacggc
     8821 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     8881 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     8941 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     9001 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     9061 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     9121 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     9181 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     9241 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     9301 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     9361 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     9421 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     9481 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     9541 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     9601 gcatacaacg atgcaaaccg tagatcggat aaggcgttta cgccgcatcc gacatgtttt
     9661 ccctcaaatc agaacaacag cgccagcccg ccgacaatca caccagacac tgccaccatc
     9721 gcccctgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     9781 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     9841 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     9901 gccaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     9961 aataaccaga cgcgagcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
    10021 ctccagaaaa gcgtccatag cgccctgccc cagcggctaa aaaaaccgcc ctgtgcttcc
    10081 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
    10141 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
    10201 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
    10261 cagaatgcca gcgcaccgcc catcgacacc tgttggcgac gcattcccgc cgcgaccgga
    10321 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
    10381 cgaaagcgcg attgcccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
    10441 aacgaaccga ggatcacccc cagcactgcc gctttccata ccgcgaggaa atagatcatc
    10501 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
    10561 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
    10621 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
    10681 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
    10741 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
    10801 aattctgaag agcttaaaat cgtcacacct tcatgcccgg gccgcatcgc ctctgccagc
    10861 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggtaa taaacggaac
    10921 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag taacatcgac
    10981 gggcgagcaa tggtcaattt cggccagttc tgggcgatta atgcttcttc catctcccct
    11041 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
    11101 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
    11161 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
    11221 acaatgtcga tgggatcggt gacctgcgcc agcgcgtcag tcagttgcgg atcatgggga
    11281 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
    11341 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
    11401 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
    11461 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgggaaa ttcgcctcat
    11521 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cactgatgaa
    11581 tttgaagatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
    11641 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
    11701 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
    11761 ggcggccatt caccggatta tctgcgtggg gacaaccgtt ttgtcacctt tacccgtgat
    11821 tttgtgaata gtggcaaacc ggtgtttgcc atctgtcacg gcccgcagtt gctgatcagc
    11881 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
    11941 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
    12001 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
    12061 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
    12121 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
    12181 attgtacgcc ttgcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
    12241 aaactgcaca ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
    12301 tgccgcctgc ggcccgctca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
    12361 ggcgactttc tgcgcatcaa aaagataaaa ggcattagcg caccagagtt ccccttcctg
    12421 ctgcacacac caggtgacaa catgttgttt tgccagccaa cggctgatgg cgatgagtgt
    12481 ttccattgct gttctctttt atactgtggg cctgacctta acatgcgatg cagacgatga
    12541 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
    12601 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
    12661 ggaaaggaga actgacgctg gcgttttctg ctccagtggg cgatcgttcg ctggcgttac
    12721 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
    12781 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
    12841 atgctcgtga tactcaacca ggccggtaac gccattcagc gcgtcatccg ctaaacgatg
    12901 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
    12961 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
    13021 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
    13081 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
    13141 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
    13201 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
    13261 ttcgaatgag cgacgtagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
    13321 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
    13381 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
    13441 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
    13501 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
    13561 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
    13621 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
    13681 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
    13741 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
    13801 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
    13861 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
    13921 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
    13981 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gatagcgcaa
    14041 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    14101 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac caatgcccgt
    14161 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    14221 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    14281 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    14341 gacctcgcca tgctggaata cgccgccgag cgttatccgc atattgagcg ccacgtatcg
    14401 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgacgtt
    14461 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacaact ggcacgggtc
    14521 acacctgtac cactggaagt ctttgctttc ggcagcctgt gcattatgtc ggaaggtcgt
    14581 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtaggcgc gtgttctccg
    14641 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    14701 ctgatcgacc gttatcagga cggcgaaaac gcaggttatc cgacgctatg taaagggcgt
    14761 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    14821 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    14881 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    14941 aaggccgatc cgcaaaactt cataccgcaa agcgcgtgga tggagacgct cgggtcgatg
    15001 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    15061 tgaaatattc cttagggcca gtgctgtggt actggccaaa agagacgctg gaagattttt
    15121 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    15181 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagtg
    15241 gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    15301 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    15361 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    15421 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    15481 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagctgggc attcgcaacc
    15541 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    15601 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    15661 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    15721 agaccatgag cggctacgtt tacaacctcg gtaacgagct ggcatccatg caggggctgg
    15781 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    15841 gcgctaatga aaatggcgct gcgccactat tgttgacggc aaacagcgac tgtaacggct
    15901 actggcggcg gctggcagga ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    15961 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    16021 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    16081 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    16141 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    16201 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    16261 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    16321 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    16381 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    16441 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    16501 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    16561 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    16621 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    16681 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    16741 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    16801 gcgatggtgt gcatcaatat tatcgccgcc gatagcaacc gcgacgctga gtttctgttt
    16861 acctcaatgc agcaagcctt tgtgaagttg cgccgtggcg aaaccgggca actgccgcca
    16921 ccgattcaaa atatggatca gttctggtcg ccgtccgaac agtatggtgt gcagcaggcg
    16981 ctgagtatgt cgttggtagg tgataaagcg aaagtgcgtc atggcttgca gtcgatcctg
    17041 cgcgaaaccg acgccgatga gattatggtc aacgggcaga ttttcgacca ccaggcgcgg
    17101 ctgcattcgt ttgagctggc gatggatgtt aaggaagagt tgttgggata gtgtgtctta
    17161 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    17221 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    17281 cggcagtaaa ttaaagctcg ataaaatatg gaccagtgcg ttgccaacgc caaacaccag
    17341 aatcagcgta atcatcggct tgccgcccca gacgcggaat ttcgggctgc caaagcgttt
    17401 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    17461 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    17521 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    17581 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    17641 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    17701 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    17761 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    17821 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggcttgg
    17881 cacgttaccg tgataaccaa acgacgccag gcagaacggc aatgtcatca gcagatacgg
    17941 cgcataagat gcattgcttt ctgcaacgtt gaacaatgtc gcaggctgca catgccccaa
    18001 caggctgcca aaggtgagga agaaggtaat gactttcgcc cccagcacaa tcgccgtcat
    18061 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    18121 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    18181 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatgcc
    18241 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atagtgtcaa aactcgaacc
    18301 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    18361 ggtgaagatc agcgccgcca ttgaccagaa gaaccacgcc ccggacatga ccactggcag
    18421 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    18481 cggtgacgtt tgggtggtgg ttagtgttgc catgagggct tctctccagt gaaaaatagt
    18541 gcgactgcgt tgttatgtat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    18601 gccccgatgg ttcaaatcgg ggctgtatat attattttac agactgtgtt cgctgttcag
    18661 cgatgattac gcatcgccac cgaaacgacg acgaccggta gagtcatcac gacgcggtgc
    18721 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacgaccac cttcacggcg
    18781 ttcaccgctg aagttacgac cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc
    18841 acgacgctca ccgccagtat gcggctgcgc atcgcccagc aactgcatat tcatcggctt
    18901 gttcagaatg cgagtacgcg taaagtgttg cagtacttca cccggcatac ctttcggcag
    18961 ttcgatagtg gagtgagaag caaacagctt aatgttaccg atgtaacggc tgctgatgtc
    19021 gccttcgtta gcaatcgcgc caacgatatg acgaacttca acaccatcat cgcggcccac
    19081 ttcaatgcgg taaagctgca tgtcaccaac atcacgacgt tcacgacgcg gacgatcttc
    19141 acggtcgcca cgtgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    19201 gaattcacgt ttcggacgca tcggcgcatc tggcggaaca atcagagtac gttcaccctg
    19261 tgccattttc agcagtgccg cagccagagt ttcgagatcc tgctcttcac cttctgcagt
    19321 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    19381 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    19441 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    19501 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    19561 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    19621 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    19681 tttcaggcgt tccagtgtct gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    19741 actgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcat ttttggtacg
    19801 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    19861 tttacgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    19921 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    19981 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    20041 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    20101 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    20161 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    20221 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    20281 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    20341 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    20401 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    20461 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    20521 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    20581 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    20641 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    20701 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    20761 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    20821 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    20881 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    20941 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    21001 aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    21061 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    21121 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    21181 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    21241 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    21301 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    21361 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    21421 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    21481 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    21541 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    21601 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    21661 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    21721 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    21781 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttccagaact
    21841 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    21901 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    21961 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    22021 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    22081 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    22141 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    22201 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    22261 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    22321 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    22381 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    22441 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    22501 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    22561 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    22621 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    22681 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    22741 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    22801 gttgcagtga ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    22861 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    22921 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    22981 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    23041 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg
    23101 ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    23161 ggctgccagt cccaacgtgg tttaccggct tctttcacca gttcattgat gttctgaata
    23221 acaacctgct gttgttcatg accgaacact actgcgccca gcatctggtc ttcgctcagc
    23281 agttcagctt cagattcaac catcagaaca gcggcttcag taccggcaac aaccagatcc
    23341 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    23401 cctacgcgag cagcaccaat cggaccattg aacggaatac cagacagaga cagtgctgcg
    23461 gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg ggttaacaga aaccacggtg
    23521 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    23581 atcagacgcg cgatcagggt ttcgccttcg cttgggcggc cttcacgacg gaagaagcta
    23641 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    23701 tcctgacctg gtttggcttt tttctggcca acaacggtaa caaataccgc ggtgtcatcc
    23761 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    23821 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    23881 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    23941 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    24001 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    24061 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    24121 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    24181 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    24241 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    24301 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    24361 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    24421 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    24481 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    24541 ttcaaccacc aggcgacgag gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat
    24601 aaatttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    24661 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    24721 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    24781 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    24841 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    24901 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    24961 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    25021 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    25081 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    25141 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    25201 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    25261 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgccagt
    25321 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    25381 tttttgcagc gcatcattgc tggacatacc ctgcgattta tccagcaaca aaacgccgtt
    25441 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    25501 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    25561 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    25621 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    25681 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    25741 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    25801 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    25861 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    25921 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    25981 ggcaatccat gccggatgcg gcataaacgc cttatcccgc atggaaccct aaaaacctta
    26041 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    26101 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    26161 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    26221 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatggc
    26281 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    26341 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    26401 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    26461 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    26521 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    26581 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    26641 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    26701 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    26761 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    26821 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    26881 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    26941 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    27001 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    27061 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    27121 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    27181 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    27241 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    27301 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    27361 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    27421 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    27481 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    27541 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    27601 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    27661 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    27721 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatagttt cgccgatcac
    27781 aacgtcacgg ttaacggcct gagcaggctt ctggaagcct tgctgcaggg aagaaccttt
    27841 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    27901 ttcagcgtgt ttgttacctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    27961 gccgccttcg acttcacgat cgctttcgtc ttcggcctgg cgagcatgtt gagaagtagt
    28021 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    28081 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc
    28141 ttcttcttca gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    28201 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    28261 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcggcctcac gttcagcttt
    28321 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    28381 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    28441 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    28501 gttaagggtg ctgcgtgttt tacgctgcag cgtcaatttg tccgggcctg aatttttctg
    28561 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    28621 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    28681 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    28741 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ctttttcgtc
    28801 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    28861 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    28921 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    28981 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    29041 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    29101 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    29161 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    29221 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    29281 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    29341 agcaacgtct gccggtgcca ttgcgttaat cacaaactgc gccgggttat catcccacag
    29401 gacgatatcg atacgctcgc cgcccagttc agtagaaacc gcctgaacac gcgcgccacg
    29461 catacctaca caggcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    29521 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    29581 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    29641 ttgcgcgcca cgcgcttccg ggcgaacgga atagagcacg ccacgaacgc ggtcgccagg
    29701 gcggaagttt tcacgcggca gcatatcttc gcgcaggatc acggcttcag cattgttgcc
    29761 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    29821 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    29881 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    29941 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    30001 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    30061 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    30121 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    30181 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    30241 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    30301 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    30361 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    30421 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    30481 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    30541 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    30601 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    30661 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    30721 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    30781 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    30841 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    30901 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    30961 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    31021 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    31081 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag
    31141 ttcactttca tttgttgaat acttttgcct tctcctgctc ccccttaagc gcattatttt
    31201 acaaaaaaca cactaaactc ttcctgtctc cgataaaaga tgattaaatg aaaactcatt
    31261 tattttgcat aaaaattcag tgagagcgga aatccaggct catcatcagt taattaagca
    31321 gggtgttatt ttatgacgac gattctcaag catctcccgg taggtcaacg tattggtatc
    31381 gctttttccg gcggtctgga caccagtgcc gcactgctgt ggatgcgaca aaagggagcg
    31441 gttccttatg catatactgc aaacctgggc cagccagacg aagaggatta tgatgcgatc
    31501 cctcgtcgtg ccatggaata cggcgcggag aacgcacgtc tgatcgactg ccgcaaacaa
    31561 ctggtggccg aaggtattgc cgctattcag tgtggcgcat ttcataacac caccggcggc
    31621 ctgacctatt tcaacacgac gccgctgggc cgcgccgtga ctggtaccat gctggttgct
    31681 gcgatgaaag aagatggcgt gaatatctgg ggtgacggta gcacctataa aggaaacgat
    31741 atcgaacgtt tctatcgtta tggtctgctg accaatgctg aactgcagat ttacaaaccg
    31801 tggcttgata ctgactttat tgatgaactg ggcggccgtc atgagatgtc tgaatttatg
    31861 attgcctgcg gtttcgacta caaaatgtct gtcgaaaaag cctactccac agactccaac
    31921 atgcttggtg caacgcacga agcgaaggat ctggaatacc tcaactccag cgtcaaaatc
    31981 gtcaacccaa ttatgggcgt gaaattctgg gatgagagcg tgaagatccc ggcagaagaa
    32041 gtcacagtac gctttgagca aggtcatccg gtagcgctga acggtaaaac ctttagcgac
    32101 gacgtagaaa tgatgctgga agctaaccgc atcggcggtc gtcacggcct gggcatgagc
    32161 gaccagattg aaaaccgtat catcgaagcg aaaagccgtg gtatttacga ggctccgggg
    32221 atggcactgc tacatatcgc ttatgaacgt ctgttgaccg gtattcacaa cgaagacacc
    32281 attgagcagt atcatgcaca tggtcgtcag ttgggccgtc tgctgtacca ggggcgttgg
    32341 tttgactccc aggcgctgat gctgcgtgac tctctgcaac gctgggttgc cagccagatc
    32401 actggtgaag ttaccctgga gctgcgccgt gggaacgatt attcaatcct gaataccgtc
    32461 tcagagaacc tgacctacaa gccagagcgt ctgacgatgg aaaaaggcga ctcggtgttc
    32521 tcgccagatg atcgtattgg tcaattgacc atgcgtaatc tggatatcac tgatacccga
    32581 gagaaacttt ttggttatgc caaaactggc ctgctctcct cctctgccac ttcaggcgtg
    32641 ccgcaggtgg agaatctgga aaacaaagtt aagtaatatc atagctttcg tgctaaaggc
    32701 cgcaaatgcg gccttttttt acggctaaga gaaagtgact gcgtttaggg gccaccatgt
    32761 ccgaattcgg aattaaagta aaagttaaat gaaataaagt aaatttcagc gcattctatt
    32821 tttactttat gtttttatgc tcagtaaaag gaatgaccga tgaatggtga atattttgta
    32881 cgcctcgcat tacatactct gaaatgcact caaaaagatt tagcaagcca cttaggtgtt
    32941 tcctcaacac aaattagcaa gtggaaaaaa ggggaacaca tgtccgctga tatggaaaaa
    33001 aagttcagaa atattacaca aataggagat tattctcctc aactagttga atggacgggc
    33061 tcagtcgcga atgctgagaa atgggatcga cttatccatt ttcttgctcg acgagctatg
    33121 gacgatgctg aaacgggtta tataacacaa cttcttactg acgaagatgg ttttttggtt
    33181 gaagaaacaa ttgatgtatt aaagcgaata ggttttccta cacctctttc ctttcctgaa
    33241 ggacttaata ttgacgacga taatgctgat gaagaagagg ctttttggga gatattagag
    33301 agcaatgctc attgttctgt aattaatgat atttatcatg ctctgaatga tgtttatggt
    33361 ttctacatag catatgttga tgagttgatt caggacgacg atttagatgt ctattcaagc
    33421 gaagccatca atattcagag ttcattgata tcacttgctg catgcaaaat tgaaattgat
    33481 actcctgtt
//
LOCUS       DAZFRN010000039        33348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFRN010000039 DAZFRN010000000
VERSION     DAZFRN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.039"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHKUB_001814"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHKUB_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO6654840.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="ACHKUB_001815"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="ACHKUB_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO6654841.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHKUB_001816"
     CDS             complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHKUB_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO6654842.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHKUB_001817"
     CDS             complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHKUB_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6654843.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHKUB_001818"
     CDS             complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHKUB_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO6654844.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACHKUB_001819"
     CDS             complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACHKUB_001819"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO6654845.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHKUB_001820"
     CDS             complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHKUB_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO6654846.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHKUB_001821"
     CDS             complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHKUB_001821"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO6654847.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHKUB_001822"
     CDS             complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHKUB_001822"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO6654848.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHKUB_001823"
     CDS             complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHKUB_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO6654849.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHKUB_001824"
     CDS             complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHKUB_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO6654850.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHKUB_001825"
     CDS             complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHKUB_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO6654851.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11269..12309
                     /gene="gpr"
                     /locus_tag="ACHKUB_001826"
     CDS             11269..12309
                     /gene="gpr"
                     /locus_tag="ACHKUB_001826"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO6654852.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACHKUB_001827"
     CDS             complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACHKUB_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO6654853.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13034..13918
                     /gene="yghA"
                     /locus_tag="ACHKUB_001828"
     CDS             13034..13918
                     /gene="yghA"
                     /locus_tag="ACHKUB_001828"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6654854.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14190..14615)
                     /gene="exbD"
                     /locus_tag="ACHKUB_001829"
     CDS             complement(14190..14615)
                     /gene="exbD"
                     /locus_tag="ACHKUB_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO6654855.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14622..15356)
                     /gene="exbB"
                     /locus_tag="ACHKUB_001830"
     CDS             complement(14622..15356)
                     /gene="exbB"
                     /locus_tag="ACHKUB_001830"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6654856.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15608..16795
                     /gene="metC"
                     /locus_tag="ACHKUB_001831"
     CDS             15608..16795
                     /gene="metC"
                     /locus_tag="ACHKUB_001831"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO6654857.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16935..17594
                     /gene="yghB"
                     /locus_tag="ACHKUB_001832"
     CDS             16935..17594
                     /gene="yghB"
                     /locus_tag="ACHKUB_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO6654858.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17634..18533)
                     /gene="yqhC"
                     /locus_tag="ACHKUB_001833"
     CDS             complement(17634..18533)
                     /gene="yqhC"
                     /locus_tag="ACHKUB_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO6654859.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18727..19890
                     /gene="yqhD"
                     /locus_tag="ACHKUB_001834"
     CDS             18727..19890
                     /gene="yqhD"
                     /locus_tag="ACHKUB_001834"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO6654860.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19995..20822
                     /gene="dkgA"
                     /locus_tag="ACHKUB_001835"
     CDS             19995..20822
                     /gene="dkgA"
                     /locus_tag="ACHKUB_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO6654861.1"
                     /translation="MANPTVIKLQDGNVMPQLGVGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21022..21948
                     /gene="yqhG"
                     /locus_tag="ACHKUB_001836"
     CDS             21022..21948
                     /gene="yqhG"
                     /locus_tag="ACHKUB_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO6654862.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21999..22256
                     /gene="yqhH"
                     /locus_tag="ACHKUB_001837"
     CDS             21999..22256
                     /gene="yqhH"
                     /locus_tag="ACHKUB_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO6654863.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22299..24518)
                     /gene="ygiQ"
                     /locus_tag="ACHKUB_001838"
     CDS             complement(22299..24518)
                     /gene="ygiQ"
                     /locus_tag="ACHKUB_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO6654864.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24629..26041)
                     /gene="ftsP"
                     /locus_tag="ACHKUB_001839"
     CDS             complement(24629..26041)
                     /gene="ftsP"
                     /locus_tag="ACHKUB_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO6654865.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26116..26853)
                     /gene="plsC"
                     /locus_tag="ACHKUB_001840"
                     /gene_synonym="parF"
     CDS             complement(26116..26853)
                     /gene="plsC"
                     /locus_tag="ACHKUB_001840"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO6654866.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27086..29344)
                     /gene="parC"
                     /locus_tag="ACHKUB_001841"
     CDS             complement(27086..29344)
                     /gene="parC"
                     /locus_tag="ACHKUB_001841"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO6654867.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29890..30372)
                     /gene="ygiV"
                     /locus_tag="ACHKUB_001842"
     CDS             complement(29890..30372)
                     /gene="ygiV"
                     /locus_tag="ACHKUB_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO6654868.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30425..30817)
                     /gene="ygiW"
                     /locus_tag="ACHKUB_001843"
     CDS             complement(30425..30817)
                     /gene="ygiW"
                     /locus_tag="ACHKUB_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO6654869.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30969..31628
                     /gene="qseB"
                     /locus_tag="ACHKUB_001844"
     CDS             30969..31628
                     /gene="qseB"
                     /locus_tag="ACHKUB_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO6654870.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31625..32974
                     /gene="qseC"
                     /locus_tag="ACHKUB_001845"
     CDS             31625..32974
                     /gene="qseC"
                     /locus_tag="ACHKUB_001845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO6654871.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(33020..33337)
                     /gene="ygiZ"
                     /locus_tag="ACHKUB_001846"
     CDS             complement(33020..33337)
                     /gene="ygiZ"
                     /locus_tag="ACHKUB_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO6654872.1"
                     /translation="MQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICSIPSVS
                     GEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFI
                     FCY"
BASE COUNT         8185 a         8914 c         8435 g         7814 t
ORIGIN      
        1 ataatcagta cggggttgct acaacatgta ttactttttc accacaatta acggttcaat
       61 atcactctct tttttgatga ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttaccg ctggttttgt ccagcacctc
      241 ttcatgatgg ctgacgaggt cgatattgct gccacagcga ccggcgatcg gtttcactgc
      301 gtaacctgtt ttcaccagtt catcattaac agtgaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 cagcggctca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcgtt
      481 ttgcggatga ccggtacgga ttggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gccgtccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccagcccgc gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccagttcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacat gaggcagagt cggcgttgta
      901 ctcgtaaacc ttcagaccac gctcatccat gcagaaatcc atacgaccgg tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg ggatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa tcagctcctg ctctgcgctc tcggtaatgg tgtagtaatg
     1141 atacggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gtagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccaggcgcg ctccgctgat
     1261 tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcggtat cttccgtctg
     1321 gatcatccag cccagaatgg tggtgtcatc aaacgtgtct ttaagggtat agcagccgtt
     1381 ttcgaccacc atctccagct cgcgagtcca ctgttgccct tgcggcaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgcaat tgggtaatga tggcgacatg
     1501 gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta
     1681 attcagaaag agaaaacggc gagcaaattc aacgcattgc cacttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa
     2221 cacccattgc aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tggcgctggg cgttactggt gcagagtacg acgcctggct gattcgtatt
     2341 ggcgatggcg atcaattctc cagcggcttt gtcgaagtga acccaaactc gaagatcccg
     2401 gcgctgcgcg atcatacgca taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg
     2461 ctttatctgg cggagaaatt tggctacttc ctgccgcagg atttggcaaa gcgtactgaa
     2521 acgatgaact ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc ggtgaaaatt gaatacgcca tcaaccgctt taccatggaa
     2641 gccaaacgtc tgctcgacgt gctggataag caactggcgc agcataagtt tattgcgggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaatgt ggtgttaggc
     2761 ggcgtgtatg atgccgcaga gtttcttgat gcgggcagtt ataagcatgt acaacgctgg
     2821 gcgaaagaag taggcgaacg tccggcggtg aaacgtgggc gtattgttaa ccgcaccaac
     2881 ggaccgctga atgagcagtt gcatgagcgc catgacgcca gtgatttcga gacgaatacg
     2941 gaagataagc gtcaggggta agggttggtg ttcgtcgcag caagccatcc aggccggata
     3001 aggcgttcac gccgcatccg gcaatcgtgc acaatgcctg atgcgacgct gccgcgtctt
     3061 atcagtatct acgaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag
     3121 tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg
     3181 taccagcacc cactggccca gtagatcggc agggttacct tcacaaatca gggcgatatt
     3241 cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac
     3301 agccagcacc tggcctggaa cgccaataca cattattaac tccggttatt caacttcaat
     3361 acttttgacg atcagcgaat cgccggtatc gacacgcaac cgctcgccgt gacagagcgg
     3421 acactgcgca tcgtgctgat gaatctccac cacctggctg caatcccagc accaagcctg
     3481 ggcgggttta tagacgatat gtaaatcgca cccttgcgcc accgttccgt ggcagacaat
     3541 ttcaaaacta aaacggacgg cgctctcctc aacgcaggag agcgcgccaa tttccagcca
     3601 cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc cgttggataa tttcaacggc
     3661 gctctggcaa agagacaact catgcatttt cgccactcct gcgaccaaac agcagggcgc
     3721 gacgccctgc gtgtggtaca tccggattcg tgactggcag cgaaaggatc attcgtgcgc
     3781 agtcatccgt caggcgttgg ccctcttcaa tcgacatgct gtgcgaaagc ggcgacatca
     3841 gcgaacagga gagatattgc gaaacaccgt ccagttcgcc aacggtaaag gtcatagtgc
     3901 catacggcag ttgcagacca attttttcac tcactttgcg cagcggccag agttgatccg
     3961 ggccggggaa gataactgca ctcagcatcc acggggtgat cacacacccc gtccactgac
     4021 cttcgaacag cgtaaaatca gaaacataca ccggcattga aggatgcaga aaagagagat
     4081 cgtgcatcga acgccgggca atttcttcaa acgctgcctg tacttgcgcc ttcggggagg
     4141 tctggaaacc tgctatctct tcagtcatga atcgcctccc gtgggatggc ttccacgcca
     4201 gattcacgca gcgcagccag aacctgctca agcgcaggtt caatcattgc ttcaaccgtc
     4261 ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca cgccgaccag ggtcagcttt
     4321 ttcggaaact caccggtgaa gcgcagggcc gacaatacgt cggccaggcc aagctgatgc
     4381 ggagagattt tgttggtaaa caacgccgga acttcttcat cccgcaggat catcatcgtt
     4441 cccggcgtgt tctttttcga cacaatggca tccgcaataa tcagatgatc gcgatttgcc
     4501 atgtcgccaa gcagctccat tcccgccgtg ccgccatcga ggatctcaac ataatccggc
     4561 agaatgtatc gttgctctaa cgcttcaaca atccgcacac cgatggcttc atcggtcagc
     4621 aaaatattgc cgacccctaa gactaaaata cgcattacag aaccttcact gaaaccactt
     4681 cgttgccgtc ggcatccact acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa
     4741 tggtacgcac cacttccagc ggtttattcg gatcagcaac cggtgtaccc accagcgact
     4801 gctcgtaagg accgacgtca tcattgaagt tacgcggacc agagttccag gttgatggaa
     4861 caactgcctg gtagttgctg atgataccgt ctttgatcac catccagtga gagagcatac
     4921 cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc cgttgccgga atgttcggtt
     4981 tcacaaaggt ggtgtgatcg cctttgccga tattggtgat cagtgcactg tattggtttt
     5041 gcaggatatc ctgcaattca cagcagtgaa cggtacgacc aataatacgg cccagcgtgg
     5101 agtgcagctg tgccacttcc agcgtgttgc cagtcagttt ctgataaatc gcaacgattt
     5161 cattcagttt gttctgggta gattctcgac ctgccgccag tttcaccagc atattagcca
     5221 gcggacccac ttctaccgtt ttgccgtaga aagtcggtga tttcacccag gaatatttcc
     5281 cgtcgtcaga ccaaccatca taagccggaa tggtggtacc ttcccacggc gcctgtggcg
     5341 cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat gcctttgatc aggtattcat
     5401 cggaatgaga agtgatcgga cgatacgagg acagatccgc attctcaatg tagccgcccg
     5461 ggaacaggaa gctgccgttt ttgctgtcgg tcgggaattc cggcacgctc aggtagttca
     5521 ccgcaccttt accgcgcgtc agccattccg ggtagaacgc ggcgataacc gcggtatcaa
     5581 ccttataaac ctgctcaaca aagtcgctca gtttgtcgat gaaagacttg atgtacatca
     5641 ggcgctcaag gttcaacacg cccaacccgt cgaggttgat tgggttcgcg acaccaccca
     5701 ctgccaggtt ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg cggttagcgt
     5761 cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc aatcaggttc acttccggcg
     5821 gcagtttcat cgctgggtga ccccagtagc cattagcgaa aatacccaac tgaccgctgg
     5881 caaccagatc tttgatcttg ttctgaactt tggtgaactc ttccgggctg ttcagatgcc
     5941 aggtcgaaac gcctttcagc atttcggagg ctttggttgg gtcagcttgc agtgcagaag
     6001 tgatatccac ccagtccagc gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg
     6061 tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt aaccggaacg tcgatattca
     6121 gcgcactttc tgccgcacga acggaagaca gcgcgtgagt ggtagtacat acgccacaga
     6181 tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc
     6241 cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac gccattttcg atttcgcaat
     6301 cgatgcgtaa atgcccctca atacgggtta ccggatcaat agtaattctc tggctcatgc
     6361 tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag
     6421 acgaatgagt acgatgtaag cgcaaatctc aatagccaca aaaccaatag aaatcaacag
     6481 ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca ccagcgaata
     6541 ggtcagacgc caggttgcac aacctaacag tgcgctcagt gctgacagga acagcatgcg
     6601 ggaatcatta cgcagcttcg ccacacgcag aaccaccagc gggaagagca tcagcaggac
     6661 ttcaatccag aacatcacgg agtagaagtc accggcaaac gctaacgaca gcttgtcgcg
     6721 atagataagc tcgccaaagc gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt
     6781 ggtcagcttg acgaacagac tcttttcatc cggaccgttg ccacgcagac ccgcctgcac
     6841 cagcgaacct tcaaagatga caatcgagaa gcccatgatg aacgccgtca gcagcgagaa
     6901 cagcggcaac atttcatagc tctgccacaa cggatgcacc ttgtagcccg ccgagatcat
     6961 cagtgacccc attgaagact ggtgcatggt cggcaacagc gcgccaagtg caatgatgaa
     7021 gaacatcacc ttgttcagac gcttgagaga aaccttccag cccagacgtt caaacagtgc
     7081 cggagcaaac tccagtgcca tcacgccgat atagatggtc atacagaccg ccgtctcgaa
     7141 cagtaccgag ttcacgttga agtgacccgg aatgtagaag tacggcaggt tccagtagcg
     7201 acccacgtcg atagtgatcg acaagccccc cagtgagtaa ccaaacagac tcgccaacag
     7261 cgccggacgc accagcggat ggtattgccc acggttaaag acgtataccg cccacgccag
     7321 cgcccagccg ccacaggcaa agccggtgcc aatcagcagg tcaaacgcga tccacacgcc
     7381 ccacgggaag ccgccgttca ggtcagagac agagcccaga ccgaacacca gacgcttcac
     7441 aatcaggagc atacagatga cgattaacgg tccaaaaatc atgaccggtt tactgatgat
     7501 tttgccgccc agcggttgtg gatcatgact catgatcgtc tcctccgtcg tgatggtcgt
     7561 ttttggtgtt gcgacgaacc agcacggtta agcccgccag cacagccagt ggtagcatca
     7621 tgcctttata cagggtgtgt tgaatatttt cggaacgcgc accggtagaa agatcgtcca
     7681 gtttcggcag gtcgagattt tcataaggca cacccgtcag taccagaacc tgagtaccgc
     7741 cgccctcttt ctcgccgtac agatgcggat aatatttcgg caccgtatgc aggtaagtgt
     7801 cgccagattt cagcgtctga cgcggatagt ggtattcgct gccaggcttc agcgccagac
     7861 gttttttcgc ctccgccatc agctcttcac gcgtaccaaa aattaccgcg cccgccgggc
     7921 acacttctac gcagccaggc aaaccgcctt tatcgagacg ttccacacct ttctggttgc
     7981 acaactcgca cttatgcagc gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt
     8041 atgggcaggc gaccatgcag taacggcaac cggtgcacac atctttgtcg taatggacaa
     8101 tgccggtttt cggatctttt ttcagcgcag agaccgggca cacagagaca cagttcggat
     8161 cgacgcagtg catacactgt ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt
     8221 tgacccctgt gccgctggtc cacacctgaa tgatgttatt ggtatacggc gacagtttgt
     8281 cgttgttcga ccaggtctgt tccccttgcg ggttacgttc agggaagttg atatcctgac
     8341 acttggtgac gcaagcctgg cagcctacgc acaaggtcga gtcgtacaac atccccagcg
     8401 atcccggaat tggcgggcgg ttttcagcag ccgcatgact gacagacggc agcgcgcccg
     8461 tcagcaatgc cccgcaggag gctgctttaa taaaattacg tctgttcacg gttattctcc
     8521 ccgtgagtca gcgttatctt tcttttgctg acgacccagt tcacgcaccg ccatcacgct
     8581 gacaccggca accagcccaa ccacaccgcc gagcaaacca atagcgcctg cagagacgtt
     8641 gccgccctct ttagcgttaa catccggttt ctgtgaacgc ggagtctgat tttcgacgtt
     8701 ggcaagctga tggatgcctt tatggaagcc gataccttct tcgttacagc catagcatgg
     8761 gtgaccaatc gctaccggcc acacgccgcc aacatcgcag aattgcagcg ttgagcagtt
     8821 gccgtaagtt tctggccctt tacagccgag gtggtacagg caccagcctt cacggtggcc
     8881 ttcatcaccg aactctttgg caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg
     8941 ttcgtgaatc agacggccat aggcgaaggt cggacgattt ttgtcatcca gtttcggcgg
     9001 tttgccgtaa gtgatgatgt gcgcaacggt agcgaggaag ttgtgcgggt tcggcgggca
     9061 gcccggaata ttgataacgg ttttgcctgg cagaacttct tgcaggctga ctgcgccagt
     9121 tgggttaact ccagctgcgg caacaccgcc ccacgcagag caggaaccga tagcgataat
     9181 cgctgctgcg ccttctgccg ctttgcggat gtgatccaca atcggctcac cagcaaccat
     9241 gcaataaata ccgttatctt ttaatgggat ggaaccatcc acaaccaaca catactgccc
     9301 tttgtacttc tcaagtgcgt tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga
     9361 aagcacttcg tgatactcca gagagatagt ctccagcacg aggttttcta ccgttggatg
     9421 cgttgcacga agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac
     9481 tggcggacgc tgcgggttag taaccgattc ggccatctct gcagcggctt tgctacttaa
     9541 ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg
     9601 agaatggatg agggtgttat ctccagtcat ttatagttat tccgttgcga agacctggca
     9661 tatattttgc ctcaatcgca aaatcaataa tgcgatcgat gcgccattta ccacacattt
     9721 attatggtta tcgatatcat gatactgcgg tggggcgcta aaacgaaggg aagaagcatt
     9781 agtgtaatta attaaagcaa gataatacgt atgtttgatc aattttcgag gcgataaccg
     9841 gccataaaaa agcgcaacgt agaacaggaa ttattctcac gaaagaaatc cattaccatc
     9901 tctttatcca gtccatagcg gcgcgtcaga atacccgctt cccaggcgct aagctggcga
     9961 tctccggtct tttcgtacat ccggtgtgag tagcctaata caaaaccgcg tttataatcg
    10021 gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt
    10081 aagcctttac caaaatggtt attcatcgtt cacctcattc gctatatata gccatatata
    10141 gcgatatttt aggcaaagaa cgacttttgc gcacgctatt ttttcatgct tgtttaagac
    10201 gtaatccagt aagagataaa cctatcagga gagatattta tcgaaccatt tcagtgtcct
    10261 ttgccaggca agatcggcgg cagatttgtc ataacggggc gtggaatcat tatggaatcc
    10321 gtgattaacc cccggataga tatacgcctc ataaacctta ttattggctt tcaacgccgc
    10381 ctcgtaagca ggccagccct cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa
    10441 aggcgcttca atcttcgcca catcggcagt gggtgcctga cgaccataaa acggcaccgc
    10501 gcaggccagt tccggatacg cgacagccgc cgcgttcgat acgccaccgc catagcaaaa
    10561 tccggtaata cccactttgc ctgccgcttg cggatagcgt tgcataaact caattgcggc
    10621 aaagaaatca ttcatcagtt tggttggatc aacctgctgt tgcagctcac gacctttatc
    10681 atcatttccc ggataacctc caacggaact taagccgtca ggtgccaggg cgatataccc
    10741 cgccttcgcc actcgccgtg ccacatcttc gatatacgga ttcagtccac gattctcatg
    10801 caccactacc acggctggcg ttttgccgct catttttgcg ggtttcacca gataaccccg
    10861 tacctcgccg tgaccatttg gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc
    10921 ggtaaactct acctgagtcg ccagcgcata atttggcttg agcaaatcaa acaacgctaa
    10981 tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg aggaactcac gtttcgagat
    11041 tttcccgtga gcgtaatagt cgtagtaatc caacaactct tgtgggaaat ctttggcggt
    11101 taaacgcggc atcgttgcac tcctcaactg gtgttttttt aagcaaagca taagcacgtg
    11161 tttttgccca gtttttcgtc actctgtgag ccagactacg ggatacgcgc tggcgaatcg
    11221 ctaaactaga aacattgttt cgaaattgaa cggtggaaag gagaggtcat ggtctggtta
    11281 gcgaatcccg aacgttacgg gcagatgcaa taccgctatt gcggaaaaag cggtttacgc
    11341 ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc acgttaacgc gctggaatca
    11401 cagcgtgcga tcctgcgtaa agcgtttgat ttgggcatta cgcactttga tttagccaac
    11461 aattacgggc cgcctccagg aagcgcagaa gagaactttg gtcgcctgct gcgggaggat
    11521 tttgccgctt atcgcgatga actgattatc tctaccaagg ctggctacga tatgtggccc
    11581 ggcccttacg gctctggcgg ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg
    11641 aagcgtatgg ggcttgagta tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg
    11701 ccgatggaag aaaccgcctc tgcgctggct catgcggtac aaagcggtaa ggcgctgtat
    11761 gtcgggatct cctcttactc gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag
    11821 tggaaaattc cgcttttaat tcatcaacct tcgtacaatt tactgaaccg ctgggtggat
    11881 aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg gctgtattgc ctttactcct
    11941 ctggctcagg gattgctgac cggaaaatat ctcaacggca ttccgcaaga ttcacggatg
    12001 tatcgtgaag ggaataaagt tcgtggtctg acaccgaaaa tgcttaccga agccaacctc
    12061 aacagcctgc gcttattgaa tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg
    12121 gcgttaagtt ggttgctgaa agatgagcga gtgacgtcgg tattgattgg tgccagccgc
    12181 gcggagcaac ttgaggagaa cgtgcaggcg ctgaataatc tgacatttag caccgaggag
    12241 ctggcgcaga ttgatcagca tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc
    12301 gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg
    12361 acgagtcagt cggtcaagat agcccatcac aaatgcagaa agcacaaacg tcagatggat
    12421 aatgacgtac cacatcagtt tgttatccgg gacatttttc gcatccataa agacgcgcag
    12481 taggtgaatg gaagaaattg ccacaatcga cgctgctact ttgtttttca gcgacgttgc
    12541 gtccattttc cccagccagt tcagcttctc tttgttctcg gagatatcca gctgtgagac
    12601 gaaattctca taaccggaaa acatcaccat caccagcaaa ccgccaacca gcgtcatatc
    12661 caccagcgac agcaacacga ggatcaaatc tgattccgcc atcgagaaga tattcggcag
    12721 tacgtgaata atctcctgga agaacttcag cgccagggca actaacgcca gcgaaaggcc
    12781 aaagtacacg ggggcaagca gccagcgaga agcatacatt gcattttcaa gaaaacgttc
    12841 catacattcc tgtcgtgtag ttaaaacggg caacagtata tcgcaattga gtaaacagca
    12901 cgcaacagca aatcaacata tacgttaaac ctttgtcgca taattctgag gtttagggat
    12961 cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga
    13021 agggagaaaa acgatgtctc atttaaaaga cccgaccacg cagtattaca ctggtgaata
    13081 tcccaaacag aaacaaccga cgccaggcat ccaggcgaag atgacaccgg taccggattg
    13141 cggcgagaaa acctatgttg gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg
    13201 gggcgattcc ggaataggtc gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt
    13261 ggcgatcagt tatcttcccg tggaagaaga agacgctcag gatgtgaaaa agatcattga
    13321 agaatgcgga cgcaaagccg ttctgctgcc aggcgattta agcgatgaga aattcgcccg
    13381 ttcgctggtt cacgaagcgc ataaggcgtt aggcgggctg gatattatgg cgctggtcgc
    13441 cgggaaacag gttgccattc cggatattgc agacctcacc agcgaacagt ttcaaaagac
    13501 ctttgccatt aacgttttcg cgctgttctg gctaacccag gaagcgatcc ccctgctacc
    13561 gaaaggtgca agtatcatca ccacttcgtc aatccaggca taccagccaa gtccgcattt
    13621 actggactat gcggctacga aggcggcgat tctgaactac agccgtggcc tggcaaaaca
    13681 ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca ggcccgatct ggacagcact
    13741 gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag tttggtcagc aaacgccgat
    13801 gaagcgtgcg gggcaaccgg cggaactggc ccctgtatat gtttatctgg caagtcagga
    13861 gtcgagctac gtcaccgcag aagtgcacgg tgtgtgcggc ggcgagcatt taggttaaaa
    13921 aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa ttcactctta cctgatgtgg
    13981 cgaaaacgcc ttatccgcct acaaacgcat gaagattcaa catgttgcaa tagattttgt
    14041 aggcctgata agcgtagcgc atcaggcatt gagcacctaa tgccggatgc gtcgtgaacg
    14101 ccttatccgg cctacaaacg cacgaagatt caacctgttg caatagattt tgtaggcctg
    14161 atgagcgtag cgcatcaggc aattctactt tacttcgctt tggcggtttc ttcgccgacc
    14221 agacctatct tcaggtaacc cgcctgatgc agcgtatcca ttaccttcat caacgtctcg
    14281 taatcgacgg ttttgtccgc tcggaagaag atggtggtgt ctttcttgcc ttcggttaac
    14341 gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat cgttaccgat aaacatcgag
    14401 ttgtctgcct tcaccgacag ataaaccggt ttttccgggc gcggctgcgg cgtgctggta
    14461 gaagcaggca agttcacctt cacatctacc gtcgctaacg gtgccgccac cataaagata
    14521 atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt tgatatcatg catttcgccg
    14581 ttatcgtcga ggttttcgtt aagatgcatt gccatcggat attatcctgc gcgtaatttt
    14641 tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca gatccaggtc acggctttgc
    14701 agcaacaata cctgcgctgc aacatcaccc agcatcgctt taaagccgcc aatctggcgt
    14761 gcaaatacgt tatagataac gaccgcagga atcgctgcca cgaggccgat tgccgttgct
    14821 aacagagctt ctgcgatacc cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg
    14881 ataccaataa agctgttcat gatgccccag accgtaccaa acagaccaac gaacggagaa
    14941 atcgcgccga tggttgccag atagccgtta ccgcggccca tttgacgacc cactgcggcg
    15001 acccgacgct ccagacggaa gctggtacgt tctttaatac cttcgttatc gtcgctgcct
    15061 tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta
    15121 ccaaaatcag cggcgatatc gttggcctgg tttaaggaac gcgcttcagc cagcagttgc
    15181 tgctcgcgct taaggcgacg cttctgattg aagaactcta cgctcttact gaagaagatt
    15241 gcccaggtga ctacggaggc caaaataagc ccaatcatca cgcacttaac gacaatatcg
    15301 gcgtgctgat acatacccca gacggaaagg tccgtctgca ttaaattatt acccactgtg
    15361 tatctccagg acgcaagtca caaaatctgc gcataataat atcaaaatga cgtcgaattg
    15421 atagtcgttc tcattactat ttgcatactg ccgtaccttt gctttctttt ccttgcgttt
    15481 acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt ttctgcttta tgccaattct
    15541 tcaggatgcg cccgcgaata ttcatgctag tttagacatc cagacgtata aaaacaggaa
    15601 tcccgacatg gcggacaaaa agcttgatac tcaactggtg aatgcaggac gcagcaaaaa
    15661 atacactctc ggcgcggtaa atagcgtgat tcagcgcgct tcttcgctgg tctttgacag
    15721 tgtggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg
    15781 gcgcggaacg ttaacccatt tctccttaca acaagcgatg tgtgaactgg aaggtggcgc
    15841 aggctgcgcg ctatttccct gcggggcggc agcggttgct aattccattc ttgcttttgt
    15901 cgaacagggc gatcatgtgt tgatgaccaa caccgcctat gaaccgagtc aggatttctg
    15961 tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc
    16021 cgatatcgtt aagcatctgc agccaaacac taaaatcgtg tttctggaat cgccaggctc
    16081 catcaccatg gaagtccacg acgttccggc gattgttgcc gccgtgcgca gtgtggtccc
    16141 ggatgccatc attatgatcg acaacacctg ggcagccggt gtgctgttta aggcgctgga
    16201 ttttggcatc gatgtttcca ttcaggccgc taccaaatat ctggttgggc attcagatgc
    16261 gatgattggc actgccgtgt gcaatgcccg ttgctgggag cagctgcggg agaatgccta
    16321 tctgatgggc cagatggtcg atgccgatac cgcctatata accagccgtg gcctgcgcac
    16381 attaggtgtg cgtttgcgtc aacatcatga aagcagtctg aaagtggctg aatggctggc
    16441 agaacatccg caagttgcgc gagttaacca ccctgctctg cctggcagta aaggccacga
    16501 attctggaaa cgagacttta caggcagcag cgggctattt tcctttgtgc ttaagaaaaa
    16561 actcagtaat gaagagctgg cgaactatct ggataacttc agtttattca gcatggccta
    16621 ctcgtggggc gggtatgaat cgttgatcct ggcgaatcaa ccagaacata tcgccgccat
    16681 tcgcccccaa ggcgagatcg attttagcgg gaccttgatt cgcctgcata ttggtctgga
    16741 agatgtcgac gatctgattg ccgatctgga cgccggtttt gcgcgaattg tataacattg
    16801 ccacttttgg acaattttgc agacatttaa ttgtgaaaag tcttaaattg ttgcgtccgg
    16861 gatcaaggcg tcccggacga atcaggagta caataggcag ataaaggctt aaacgctgtt
    16921 ccacaggaaa gtccatggct gttattcaag atatcatcgc tgcgctctgg caacacgact
    16981 ttgccgcgct ggcggatcct catattgtta gcgttgttta ctttgtcatg tttgccacgc
    17041 tgtttttaga aaacggcctg ctgcccgcct catttttgcc aggcgacagc ttgttgatac
    17101 tggcaggcgc attgattgcc cagggggtta tggattttct gcctacgatt gcgattctga
    17161 ccgccgcagc aagtctgggc tgctggctaa gttatattca ggggcgctgg ttagggaata
    17221 ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa atatcaccag cgcgccacct
    17281 gcatgtttga ccgccacggt ctgctggcgc tgctggctgg acgttttctt gcatttgtcc
    17341 gtacgctgct gccaaccatg gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt
    17401 tcaactggtt aagtggattg ctgtgggtca gcgtggtaac cagttttggc tatgccttaa
    17461 gtatgattcc gttcgttaaa cgccatgaag atcaggtaat gacgttcctg atgatcctgc
    17521 caattgcctt gttaaccgct ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat
    17581 actgtaacgc ctgacgattt tccccgttcc cggctgctgt accgggaacg tatttaattc
    17641 ccctgcatcg cccgcattct tgccgcatct tcccccggcg tcacaccgaa gtaacgttta
    17701 aactcacggc taaattgcga tgcgctttca tagccgacgc gcatcgctgc tgcgctagcc
    17761 ttcatgccgt catggatgat catcatccgc gccttatgca gacggtaatt cttcaaatac
    17821 tgcaacggcg aggtgctggt gacagactta aaattatggt ggaacgccga tacgctcatg
    17881 ttggcttctg ccgccagttg ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt
    17941 ttcagcacgc ggctaatcag actgaagtga gtctggcgac tgaccagcgc cagtaacgcg
    18001 ccgccgcaag gtccggtcag cacgtagtac agaatttcgc ggatgatctg tttgccgaga
    18061 atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc gctccgccgc gcataaaatc
    18121 tcttctgata acgtggcgga gttaatcccg ctggctgcca tcgacggctg gaaatgctca
    18181 tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa tatcgacatt gagacgcaac
    18241 cctgccagcg gcacctctga cgtggcatag gtttcgcact caaacggcaa cggcaccgtc
    18301 agcagcaggt attcattggc atcataacga aacacgcgtt cattgatata accgatttta
    18361 tgcccggaaa agagaattat gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc
    18421 gtctcgccat acaacaaacg cacatcgggc aacagttctg acaaactatt ttctttaatt
    18481 ttcagtttat taactttatc cgccagcaag cggcaaatct cttcacgttt catatcgcgt
    18541 aatttcttag gaataatgcg gcaatttgat tgtgcgcaat tttgtagcat ttctccagca
    18601 ctctggagga ataggcaaga cattggcaga aatgagcatt gagagccagg gcgctggcga
    18661 tcacaatgaa aaacatcagg cagatcgttc tctgccctca tattggccca gcaaagggag
    18721 caagtaatga acaactttaa tctgcacacc ccaacccgca ttctgtttgg taaaggcgca
    18781 atcgctggtt tacgcgaaca aattcctcac gatgctcgcg tattgattac ctacggtggc
    18841 ggcagcgtga aaaaaaccgg cgttctcgat caagttctgg atgccctgaa aggcatggac
    18901 gtgctggaat ttggcggtat tgagccaaac ccggcttatg aaacgctgat gaacgccgtg
    18961 aaactggttc gcgaacagaa agtgactttc ctgctggcgg ttggcggcgg ttctgtactg
    19021 gacggcacca aatttatcgc cgcagcggct aactatccgg aaaatatcga tccgtggcac
    19081 attctgcaaa cgggcggtaa agagattaaa agcgccatcc cgatgggctg tgtgctgacg
    19141 ctgccagcaa ccggttcaga atccaacgca ggcgcggtga tctcccgtaa aaccacaggc
    19201 gacaagcagg cgttccattc tgcccatgtt cagccggtat ttgccgtgct cgatccggtt
    19261 tatacctaca ccctgccgcc gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac
    19321 accgtggaac agtatgttac caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa
    19381 ggcattttgc tgacgctaat cgaagatggt ccgaaagccc tgaaagagcc agaaaactac
    19441 gatgtgcgcg ccaacgtcat gtgggcggcg actcaggcgc tgaacggttt gattggcgct
    19501 ggcgtaccgc aggactgggc aacgcatatg ctgggccacg aactgactgc gatgcacggt
    19561 ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgag
    19621 accaagcgcg ctaagctgct gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc
    19681 gacgatgagc gtattgacgc cgcgattgcc gcaacccgca atttctttga gcaattaggc
    19741 gtgccgaccc acctctccga ctacggtctg gacggcagct ccatcccggc tttgctgaaa
    19801 aaactggaag agcacggcat gacccaactg ggcgaaaatc atgacattac gctggatgtc
    19861 agccgccgta tatacgaagc cgcccgctaa gctttttacg cctcaaactt tcgttttcgg
    19921 gcatttcgtc cagacttaag ttcacaacac ctcaccggag cctgctccgg tgagttcata
    19981 taaaggagga acgtatggct aatccaaccg ttattaagct acaggatggc aatgtcatgc
    20041 cccagttggg agtgggcgtc tggcaagcaa gtaatgagga agtaatcacc gccattcaaa
    20101 aagcgttaga agtgggttat cgctcgattg ataccgccgc ggcctacaag aacgaagaag
    20161 gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga agaactgttc atcaccacta
    20221 agctgtggaa cgacgaccac aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac
    20281 tccagcttga ttatatcgac ctctacttaa tgcactggcc cgttcccgct atcgaccatt
    20341 atgtcgaagc atggaaaggc atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg
    20401 gcgtgtgcaa cttccagatc catcacctgc aacgcctgat tgatgaaact ggcgtgacgc
    20461 ctgtgataaa ccagatcgaa cttcatccgc tgatgcaaca acgccagcta cacgcctgga
    20521 acgcgacaca caaaatccag accgaatcct ggagcccatt agcgcaagga gggaaaggcg
    20581 ttttcgatca gaaagtcatt cgcgatctgg cagataaata cggcaaaacc ccggcgcaga
    20641 ttgttatccg ctggcatctg gatagcggcc tggtggtgat cccgaaatcg gtcacacctt
    20701 cacgtattgc cgaaaacttt gatgtctggg atttccgtct cgacaaagac gaactcggcg
    20761 aaattgcaaa actcgatcag ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct
    20821 aacatgcaaa ttctcccggt ggcggtaatg ttccgctacc ggacttttca gaaatcattt
    20881 attcccctcg cgtcccgccc gttgttactc ttccttgttc aggaatgcca aatataagga
    20941 catcatcatg cagagccgga agctcttaaa agaacaactc atctatatcc gggataaacg
    21001 caacggagag gtgaaaaaca gatgaaaata atacttctgt ttttagcagc cctggcaagt
    21061 tttaccgtac acgcacagcc cccctcactg accgtagaac aaacagtccg gcatatttat
    21121 cagaactata aatcagatgc cactgcccct tattttggtg aaaccggaga gcgggcgata
    21181 acttctgcgc gtattcaaca ggcgcttacc ctgaacgaca atcttacgct gccgggcaat
    21241 attggctggc tggattatga tccggtttgt gattgtcagg attttggcga tctggtgcta
    21301 gaaagcgttg cgataaccca aactgacgcc gatcatgccg atgccgttgt gcgctttcgt
    21361 atctttaaag atgataaaga aaagaccacg cagacactga aaatggtggc ggaaaatggt
    21421 cgttgggtca ttgacgatat tgtcagcaat catggcagcg tcttacaagc agttaatagc
    21481 gagaatgaaa aaacgctggc cgctttagct tcgttgcaaa aagaacagcc ggaagccttt
    21541 gttgccgaac tctttgaaca tattgctgat tatagctggc cgtggacgtg ggtggtttcc
    21601 gactcttacc gccaggcggt taatgccttc tataaaacca ccttcaagac ggccaataat
    21661 cccgatgaag atatgcaaat agaacggcaa tttatttacg acaatccgat ctgttttggc
    21721 gaagagtcgc tattttcacg cgttgatgaa attcgagtcc tggagaaaac cgccgattcc
    21781 gcccgcattc atgttcgttt tacgctgacc aatggcaaca acgaagagca agaactggtt
    21841 ttacagcggc gcgaaggcaa gtgggaaatc gctgatttta tccgcccgaa cagcggcagc
    21901 ctacttaagc agattgaggc aaaaaccgcc gccagattaa agcaatgagc tgaattaaat
    21961 aacaattagc cggaacaata aataaaaggg aacactatat gaaaacgatt ttcaccgtgg
    22021 gagctgttgt tctggcaacc tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc
    22081 agctggcgag caatgtgcaa acattaaatg ccaaaatcgc ccggcttgag caggatatga
    22141 aagcactacg cccacaaatc tatgctgcca aatccgaagc taacagagcc aatacgcgtc
    22201 ttgatgctca ggactatttt gattgcctgc gctgcttgcg tatgtacgca gaatgataaa
    22261 aaaatccccg gcagcatgtc agttgccggg gatttttttt aacgtccaac cgccgcttta
    22321 gggcgtttct tcgcaccagc attcaccgga cgagattgcg tagacgacgc tttttttgcc
    22381 gtagcaggcg tctgacgctg ggtcgccatc ggcgtatgtt tcgtcaacgc cggacgggta
    22441 ttgcggttct ggcgacgagc ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa
    22501 tcgcgacggc tgccaatcag atgctttttg cccatcgctt ccagcgcctg gcggattaac
    22561 ggccagtttg ccggatcgtg gtaacgcaac aacgctttat gcaaacgacg ctgcttgtcg
    22621 cccttcggta cgaagacgtc ttcactctta taaccaatct tcgccagcgg gtttttcccg
    22681 gtgtaataca tggtggttga gttcgccagc ggcgatgggt agaagttctg tacctggtcg
    22741 agacggaaac gatgcttttt cagccacagc gccagattca ccatatcttc atcacgcgta
    22801 ccggggtgcg cggagatgaa ataggggatc agatactgct ctttacctgc ctgttttgag
    22861 taagtatcga acagctcttt aaagcggtca tagctgccca tgcccggctt catcatcttc
    22921 gataacggcc cttcttcggt atgttccggg gcaatcttca gataaccgcc gacgtgatgg
    22981 gtcgccaact ctttgatata gcgcggatct tccacggcga tgtcataacg tacgcctgag
    23041 gcgatgagga tttttttaat gcctttcaga tcacgcgcac ggcgatagag gttgatcgtc
    23101 ggttcgtggt tagtgtccat gtgcggacaa atatccggat aaacgcacga caaacggcga
    23161 caagtttgtt cagcgcgtgg cgatttgcag cgcaacatat acatgttggc agttggccca
    23221 ccgagatcgg aaatcacgcc cgtaaaacct ggaacggtgt cgcggatcgc ttcgatctca
    23281 ttaatgatcg aatcttccga acggctctga ataatgcgcc cttcgtgctc ggtaatagaa
    23341 cagaaagaac agccgccaaa gcagccgcgc ataatgttga ccgaaaaacg gatcatttcg
    23401 taagccggaa tacgggcatt gccataggcc ggatgtggca cgcgcttgta cggcagcgca
    23461 aaaacgctgt ccatctcttc ggtagaaagc gggatagcag gcgggttgat ccacacatag
    23521 cggtcaccgt gtttttgcat caatgcgcgg gcgcagcctg ggttggtttc gtggtgcaga
    23581 atgcgcgaag catgggcgta cagcacttta tcgcccttca ctttctcgaa agaaggcagc
    23641 aacacgtagg ttttttccca cggtttcggg cgcggtggct gcacggttac ggctttggct
    23701 tcctgctttt tcggtgccac cggtttgtta tccgcgcacg gcaaatcttc accatacgga
    23761 tgcgggattg ggtcgatttt tccaggggta tcaagacggg tggaatccac gccgctccag
    23821 ccaggcagcg cctctttcac gataatcgcg gtattacgca catcgcggat ttcactaatc
    23881 ggctcgccca tcgccagacg gtgcgccacc tccaccagcg gacgctcacc gttaccaaac
    23941 atcagcatgt cggctttcga atccaccagc acggaacggc gcacggtatc ggaccagtaa
    24001 tcataatgtg cggtacggcg cagactagcc tcaataccgc cgaggatcac cggtacatct
    24061 ttccacgcct ctttacaacg ctgggtataa accagtgtgg cgcgatccgg gcgcttaccc
    24121 gcgacgttat ccggcgtgta ggcatcgtca tgacgtaaac ggcgatcggc ggtataacgg
    24181 ttgatcatcg aatccatgtt gccagcagta acaccgaaaa acagattcgg tttacccaga
    24241 cgcatgaaat cgtctttgct gctccagtct ggctgggcga tgatcccgac gcgaaagccc
    24301 tgtgcttcca gcatacgacc gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg
    24361 tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa gttgatccat ctcttcacga
    24421 gacatcggca aaaacggtgc cggtccaaaa caggcggccc agtactgcgg ccaggagaac
    24481 aggtcgcgat ccggttggat cagggagata gagctcattt tgcttccaga aatgataaaa
    24541 aaataatcaa aggccgggga ttataagccg gaacgaaaga gaaatcgaaa ggtattccat
    24601 actcgccctc ctcgggcgag tatgaagatt acggtaccgg attgaccaaa agttgcccta
    24661 tcgagccacg gtccgccatt tccagcgtct gactgttgaa gtagaacggg aagtgcgccc
    24721 aggaaggctg accgaaataa acaagcagct ccacttgtcc gtcaacccaa acggtatctt
    24781 tccagcctct gtcttccggg aacggcatcg cgccattcac gttacggatc tggaacatta
    24841 cgccttcaat atggaacgct tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc
    24901 cttgctgcgc ggtgacatca atacggttga cgtcccacag ctgtccatta atacccgggt
    24961 catcacccag actgatatcg cgactgcgaa ttggcgaacc agccatgatt tcagttggca
    25021 gcaagcgcat cggaagactg tctgtgacca gcggcagaag gccggttggg cgcagcgtta
    25081 gcaccagggt agaaaccaga atactggatg gctcaaagaa gccgcgaata cgatcaacaa
    25141 tgctcgccgc ttcgccacag gtgatcgaca cttcatcgcc gttgctcata tccaccagaa
    25201 tctcgcggcg ctcgcccggt gccagcgaaa gttgcttcac cgatacagga gcaggcagga
    25261 atccctgatc gccagaaatc acatgtaacg ggcgaccatc gctcatctgt agttgatagc
    25321 gacgagagtt cgacgcgttc agcagtcgca agcgcaccca gccacgcgag acttcaacgt
    25381 acgggctttg tacaccgtta accagcagcg tatcaccaac aaagccgccg cttcccggtt
    25441 cgttgtattc tggcgtacca aagttatcca gccgtttatc ctggataatg accggaaaat
    25501 catccacacc ataatggttg gggataggca gcgacttgct gacttcatct tccaccagcc
    25561 acattccggc aaggccgtta tagacctgct gagccgtgcg gttgggagta ttggcgtgat
    25621 accacagagt agctgcgttc tggcgaatgg gcagtacggg tgcccagtca gcgtttggcg
    25681 acatcatccg tgccggaccg cccatcagcg ggcctggtac ctgtagcccg gccaccgtca
    25741 ttgagacatt ttctgtcagg cggttgctgt aaataagctt aacatcgtcg cccttccaga
    25801 cgcggatagt cggccccagg taacgaccat tgattcccca gaccgacgcg cgtgtccctg
    25861 gcgtaaatga ccagtgcgca cgttgtacag tcataaacag cggttgccca cggcgagatt
    25921 caagtagcgg cggaacgggt agcggttgct gttgcccggc tgcgctggcc ttcaggggaa
    25981 cagcgcctgc acaaagtgca atccccgatg cctgaatgaa ctgacgccga ctgagtgaca
    26041 tatttgctcc atgtaaaact ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa
    26101 atacagattg ccgacttaaa cttttccggc ggcttcgcgt tctgcgactt ctttatcgag
    26161 ctcggcgatt ttttgttcca ttatcgaacg acaatgggca gccagctcac gaacctgatc
    26221 tttgccatac tgactgacgt caattggcgg cagcatttcg acaatcacca gaccgttgtg
    26281 cagtcgatta agattaatct tattcgaagt tgtagagacg cacacgggaa taatcgggac
    26341 gcccgccgca attgccgcgt gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg
    26401 gctgcgggtt ccttccggga acatccagat ggaaatacgg cgttttttga aatgattcac
    26461 gacttccgca atggtgccgt gagctttagt gcgattgttt ctgtcgatca ataagttgcc
    26521 ggttaaccag tacaactgcc cgaagaaggg gatccacagc aagctctttt tacctaccgt
    26581 caccgtcggc ggttgcacaa tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt
    26641 agcgatatag atagcattgc cgtagctttc agcatctgca ggtttgcggc actcaacttt
    26701 caggccgaac agcggcgcaa gacggccaaa catatgccca aaggtggcca catgtttcgg
    26761 gttacgcggg ctgaaaaggc agtaaatgga gccgaataca cagactaaga tgctgtaaat
    26821 cacggtaata ataagacgaa agatatatag cataacaccc tctgaaggtc tgagagactg
    26881 gcattgtacg tcacctgaaa tcaggttagg gcattgttaa aagccgctct ctggaaaagc
    26941 gtattgttaa tcgcaacgca tgaataaaca acggtttcaa gggaattttt atcgtttccg
    27001 ggggttgaat acgctgccgg atgacgactt aacgtttcat ccggcgttcc ttgcaagcgg
    27061 gaggaaacag gccctccccg gcatattact cttcgctatc accgctgctg gcacggcgag
    27121 gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg catcaacgta ccgcggcgtc
    27181 cacgttcgcc agtgactttc tgtaactctt ccgggcgcag tttaattttg cgtttcccaa
    27241 catgaatggt cagcgtgctt tgcggcggca gaacgtacaa ttgcgccaga ccatcttctc
    27301 cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc ttcgacagct
    27361 gcggcagatc gcttaccggg aacatcaaca tacggcctgc ctgagtgatt gccagcagca
    27421 tatcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc ggtaaggtga
    27481 tcaaagcctt acctgcacgg ttacgcgcca ccagatcgtt aaaggtgcag acgaaaccgt
    27541 aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcagc atatggtcaa
    27601 cggtcgcccc aggcggcaac gttaatttgc cggtgagcgg ctcgccctga ccacgcgccg
    27661 acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca aacactaccg
    27721 gttggttgct cttacctttc accgccgctt tgaagctatc acccgcttta taattcaggc
    27781 ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca acccatctgc gacagcacaa
    27841 tggtgacagg ttcagacggc agcatgtcgt gctcgctcat cgctttcgct tcttcgcgtt
    27901 cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc gtctgcctgc agttctttct
    27961 tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg tcgcgctctt
    28021 tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca agatgacgca
    28081 gtttcagttc gaggatcgct tccgcctggg tttccgtaag gccaaaccgc gacatcagcg
    28141 ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac ttcgtcgatg ttgagaaacg
    28201 ccaccagcaa accttccagg atatgcaggc gcttgaggac tttctccaga cgatagttca
    28261 gtcggcggcg cacggtatcg cgacggaaca ccagccattc ggagaggatt tccagcaggt
    28321 ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga tagctctttt
    28381 ccagatcggt ggtagcgaag aggtggttca tcacctgatc catatccacg cggttggaac
    28441 gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc agatcgtcaa
    28501 ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg cgcgcacctg
    28561 aaacctgatg cggtaatgcg ctgataacca ccgcgccatc ttctttcttc cacaccgcgc
    28621 gcatacgcac tgaaccacgt ccgttctcgt agattttacg gatctcggcg cgcgaagtga
    28681 taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc tgatcgagcg
    28741 tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc tacttcacgc aggttatgcg
    28801 gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc aaaatgtttg
    28861 gcagacgagc aggcagcatt ttcggctcct gcaaagtgcc gtcgaagttt ggcacccagt
    28921 cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac aaccgggatt
    28981 cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag ttcccctgac
    29041 catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc gcttcataac
    29101 aggcgctatc gccgtgcgga tggtatttac ccagtacgtc accgacggta cgggccgatt
    29161 ttttaaattt ggcgctggca ttcaggccca gttcagacat cgcatacaca atgcggcgct
    29221 gaacaggttt cagaccatca ccaataaacg gcaacgcacg gtccatgatc acgtacatgg
    29281 agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct gccatatcgc
    29341 tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata ttgccggaga
    29401 tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc cgccggacgt
    29461 gctccgacgg cgataaaggc atcaatgtgc cttgatatac aactctttgc tgtagacttg
    29521 atcggcatag agggcagcac aaggttgcgc ccatgccaca ccactcggca tgggggtccg
    29581 ctccgggcga ttcgagaagt tgaggttatg cgagtccagg gaagcctaaa gcatcaaagt
    29641 agtcgactgt aagtatacgg ttcagcccag tttagctgtg tctccaccag tgccccgcgc
    29701 ctcctgccat aagtgcggct tgctcatggg tacccccgag acggcttacg gcgcaccgga
    29761 taataaattt cgcgtataca tggtattcct taccggagaa attataagcc caacaacaat
    29821 acgcctgtgg cattgttcgc tcaaacttat cgcgagtgat ttggctcaca ctccggtaaa
    29881 gattccccat cacgccaacg gcacataaac atccgtttcc agtcgctgct ctgtcacccc
    29941 ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta cgcattttct cgccgcttgc
    30001 aggcagccag tggcgaataa tgccccatat cgtgtgtgaa atatcgtcca gctcgccaac
    30061 gtggcgggcc acggcataac gtccaccggt aagttcacca ttgctcacac cataacgatt
    30121 atcgggaatc ggttcgctaa cgctgccgca gatatcaaag cgaaacgctt ccggtgcggt
    30181 ggtggcaggg tcgtcccagg cgacgccaaa agtctggctt tggttaacag gagaaagccc
    30241 cgtctcttta cgccacatga taaatttcgc cacactgtag ttgagcaact cagggctaca
    30301 gcggtgcggc aacatcgcca cagggattga tgggaaatcg ataatgttta catccagtgt
    30361 caggtttgtc attttgcctc cctgcgaaat tattcccggg aacgcgtccc gggagcggta
    30421 acaattacgg atttactttg cggatctgtt tgacgtcaat ttcaacagaa ttccagtctt
    30481 tatcgacttc accctgaatc tcaaccgtat ctttcggcgt caccgtcaca ccgttccagc
    30541 gtttgtggtc gatatcaaca ttgatagtac cgctggcatc tttaaacacg tagagatcgt
    30601 cagagatgcg ttcaacgata ttgccgcgca gggttaccca ggtgtcgtca cgcagggatt
    30661 ttgcgctttc tacggtcgtt acgctgccgt tcggcccctg gaatcctccg gcctgacttt
    30721 gcgttgccga tgggccagaa aaaccgccct gctctgctgc catcaccggt gcgctgcaca
    30781 gggccattac tgcgattact gctgcgaatt ttttcatgtt tattactccc tttaatgtct
    30841 gtttccgagc atttaacaag atagtcctta acaacttctt aagggaaaaa aataaaattt
    30901 agtgctgtac agagcgcgtt acaacacggt ttactggcag caaatacggt tatcgcaggg
    30961 atgaaaaaat gcgaatttta ctgatagaag atgacatgct gattggcgac ggcatcaaaa
    31021 cgggccttag taaaatgggt tttagcgtcg actggtttac acaaggtcgt cagggaaaag
    31081 aggcgcttta tagcgcgcct tatgatgcgg tgatcctgga tttaacctta ccaggcatgg
    31141 atggtcgcga tattttgcgc gaatggcgag aaaaaggtca gcgtgagccg gtactgatcc
    31201 tgaccgcgcg cgatgcgctg gaggaacgtg tagaagggct gcgtctggga gctgacgatt
    31261 atctgtgtaa accttttgcg ttgatagaag tcgccgccag gctggaagct ctgatgcgcc
    31321 gaaccaacgg ccaggccagc aacgagctgc gccacggtaa cgtcatgctc gaccccggca
    31381 aacgtatcgc cacgctggct ggcgaaccct taacactgaa accaaaagaa tttgccctgc
    31441 tggaattact gatgcgtaac gctggtcggg tactgccgcg caaactgatt gaagagaaac
    31501 tgtatacctg ggacgaagag gtcaccagta atgccgttga agtgcatgtg catcatctgc
    31561 gacgcaaact cggtagtgat tttattcgta ccgtgcatgg tattggctac acattaggtg
    31621 agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg ctgacgctaa tctttttaat
    31681 tctggcctcg gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa caacggataa
    31741 cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc aagcggttaa gtacgctcga
    31801 tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg ccaaataaat taaaacacgg
    31861 tcatgttgat gacgatgcgc tgacctttgc catctttacc cacgacggca gaatggtcct
    31921 taacgatggc gataacggag aagatattcc ttatagctat caacgggaag gttttgctga
    31981 cgggcaactg gtgggcgata aagatcaatg gcgttttgtc tggatgacct cacctgatgg
    32041 caaatatcgc atcgttgttg gtcaggaatg ggaataccgt gaagacatgg cgctggcgat
    32101 tgttgccggg caattgatcc cgtggctggt cgcactgccg attatgttaa tcatcatgat
    32161 ggtactattg ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcgc tgcgtatgcg
    32221 tgaccctgac tcggaaaaac cactaaacgc gactggcgta cccagcgaag tgcgtccgtt
    32281 ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg atgatggttc gtgaacgacg
    32341 ctttacctcc gacgcagctc acgaactacg tagcccgtta acggcgctga aagtgcaaac
    32401 cgaagttgcg cagctttctg acgatgatcc gcaagcgcgg aaaaaagcgc tgcttcaatt
    32461 acattacggg atcgatcgcg ctactcgtct ggttgatcaa ctgctcactc tatcgcggct
    32521 ggactcactg gataaccttc aggacgtcgc ggagatcccg cttgaagatc tcctgcaatc
    32581 gtcggtgatg gatatttacc acacggcgca gcaggcgaaa attgacgtgc gactgacact
    32641 caatgcccac ggcatcaaac gcaccggaca accgctattg ctaagtttgt tggtgcgaaa
    32701 tttgctggat aacgccgtgc gctacagtcc acagggcagc gtgatagacg tcacgctgaa
    32761 tgctgataat ttcatcgtga gggataacgg ccccggtgtg acgccagagg ctctggcgcg
    32821 aattggcgaa cgcttctatc gcccccccgg acaaaccgct accggcagtg gacttgggct
    32881 atcgattgtc cagcgaatcg ccaaattgca tggcatgaat gttgagtttg ggaatgcgga
    32941 acaaggtgga tttgaggcga aggtaagctg gtaacttctc tgtctatgag agccgttaaa
    33001 acgactctca tagattttac taatagcaaa atataaaccg tccccaaaaa agccaccaac
    33061 cacaaaccag acaaataccg agcgaaaata catataccca aaactcgaac atttcccgca
    33121 taaagagttt tcttaagata agaataataa gtggcgtaag aagaaaaaat gctgcaatca
    33181 ttgcttgcaa tatacgatct tcgcctgaaa cggaaggtat actgcaaata ttgtctcccg
    33241 actcaagcat ccagtcatac ttattgaaag caaaaaaagc atctattaac caataagtaa
    33301 gcattatgta taacagagct tcaaatattg tttgaatttt atgcttta
//
LOCUS       DAZFRN010000040        33092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFRN010000040 DAZFRN010000000
VERSION     DAZFRN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.040"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            49..1380
                     /gene="argA"
                     /locus_tag="ACHKUB_001847"
     CDS             49..1380
                     /gene="argA"
                     /locus_tag="ACHKUB_001847"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO6654873.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(1442..3268)
                     /gene="recD"
                     /locus_tag="ACHKUB_001848"
     CDS             complement(1442..3268)
                     /gene="recD"
                     /locus_tag="ACHKUB_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO6654874.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(3268..6810)
                     /gene="recB"
                     /locus_tag="ACHKUB_001849"
     CDS             complement(3268..6810)
                     /gene="recB"
                     /locus_tag="ACHKUB_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO6654875.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDKQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(6803..9691)
                     /gene="ptrA"
                     /locus_tag="ACHKUB_001850"
     CDS             complement(6803..9691)
                     /gene="ptrA"
                     /locus_tag="ACHKUB_001850"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO6654876.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FNYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(9867..13235)
                     /gene="recC"
                     /locus_tag="ACHKUB_001851"
     CDS             complement(9867..13235)
                     /gene="recC"
                     /locus_tag="ACHKUB_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO6654877.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVPEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(13248..13571)
                     /locus_tag="ACHKUB_001852"
     CDS             complement(13248..13571)
                     /locus_tag="ACHKUB_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO6654878.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(13556..13963)
                     /locus_tag="ACHKUB_001853"
     CDS             complement(13556..13963)
                     /locus_tag="ACHKUB_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO6654879.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(13960..14523)
                     /locus_tag="ACHKUB_001854"
     CDS             complement(13960..14523)
                     /locus_tag="ACHKUB_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6654880.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(14514..14984)
                     /locus_tag="ACHKUB_001855"
     CDS             complement(14514..14984)
                     /locus_tag="ACHKUB_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6654881.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(15169..15963)
                     /gene="thyA"
                     /locus_tag="ACHKUB_001856"
     CDS             complement(15169..15963)
                     /gene="thyA"
                     /locus_tag="ACHKUB_001856"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO6654882.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(15970..16845)
                     /gene="lgt"
                     /locus_tag="ACHKUB_001857"
     CDS             complement(15970..16845)
                     /gene="lgt"
                     /locus_tag="ACHKUB_001857"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO6654883.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(16996..19242)
                     /gene="ptsP"
                     /locus_tag="ACHKUB_001858"
     CDS             complement(16996..19242)
                     /gene="ptsP"
                     /locus_tag="ACHKUB_001858"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6654884.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(19255..19785)
                     /gene="rppH"
                     /locus_tag="ACHKUB_001859"
     CDS             complement(19255..19785)
                     /gene="rppH"
                     /locus_tag="ACHKUB_001859"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO6654885.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(20138..20284)
                     /gene="ygdT"
                     /locus_tag="ACHKUB_001860"
     CDS             complement(20138..20284)
                     /gene="ygdT"
                     /locus_tag="ACHKUB_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO6654886.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            20470..21159
                     /gene="mutH"
                     /locus_tag="ACHKUB_001861"
     CDS             20470..21159
                     /gene="mutH"
                     /locus_tag="ACHKUB_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO6654887.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            21228..21941
                     /locus_tag="ACHKUB_001862"
     CDS             21228..21941
                     /locus_tag="ACHKUB_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6654888.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            22079..22297
                     /gene="ygdR"
                     /locus_tag="ACHKUB_001863"
     CDS             22079..22297
                     /gene="ygdR"
                     /locus_tag="ACHKUB_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO6654889.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            22405..23445
                     /locus_tag="ACHKUB_001864"
     CDS             22405..23445
                     /locus_tag="ACHKUB_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO6654890.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(23477..24670)
                     /gene="lplT"
                     /locus_tag="ACHKUB_001865"
     CDS             complement(23477..24670)
                     /gene="lplT"
                     /locus_tag="ACHKUB_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO6654891.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(24663..26822)
                     /gene="aas"
                     /locus_tag="ACHKUB_001866"
     CDS             complement(24663..26822)
                     /gene="aas"
                     /locus_tag="ACHKUB_001866"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO6654892.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDELGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDILYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            27408..28439
                     /gene="galR"
                     /locus_tag="ACHKUB_001867"
     CDS             27408..28439
                     /gene="galR"
                     /locus_tag="ACHKUB_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO6654893.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTSNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(28446..29708)
                     /gene="lysA"
                     /locus_tag="ACHKUB_001868"
     CDS             complement(28446..29708)
                     /gene="lysA"
                     /locus_tag="ACHKUB_001868"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO6654894.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            29830..30765
                     /gene="lysR"
                     /locus_tag="ACHKUB_001869"
     CDS             29830..30765
                     /gene="lysR"
                     /locus_tag="ACHKUB_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO6654895.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(30752..31444)
                     /gene="ygeA"
                     /locus_tag="ACHKUB_001870"
     CDS             complement(30752..31444)
                     /gene="ygeA"
                     /locus_tag="ACHKUB_001870"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO6654896.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRALTGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(31573..32991)
                     /gene="araE"
                     /locus_tag="ACHKUB_001871"
     CDS             complement(31573..32991)
                     /gene="araE"
                     /locus_tag="ACHKUB_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO6654897.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8098 a         9085 c         8226 g         7683 t
ORIGIN      
        1 cagaataaaa atacaataat ttcgaataat aatgcaaagg ggtgtgccgt ggtaaaggaa
       61 cgtaaaaccg agttggtcga gggattccgc cattcggttc cctatatcaa tacccaccgg
      121 ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa tttctccagt
      181 atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt ggtctatggc
      241 gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct gtatcacaag
      301 aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc gggaacattg
      361 caactggata ttactgctcg cctgtcgatg agtctcaata acacgccgct acagggcgcg
      421 catatcaacg tcgttagtgg caattttatt attgcccagc cgctgggcgt cgatgacggc
      481 gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgat ccatcgtcaa
      541 ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac tggcgagagc
      601 tttaacctga cctcggaaga gattgccact caactggcca tcaaactgaa agctgagaag
      661 atgattggtt tttgctcttc ccagggcgtt actaatgacg acggtgatat tgtctcagaa
      721 cttttcccta acgaagcgca agcgcgggta gaagcccagg aagagaaagg cgattacaac
      781 tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg cgtgcgtcgc
      841 tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt ctcacgcgac
      901 ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc aacaatcaac
      961 gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat tctggtgcgc
     1021 cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca gcgcgataac
     1081 acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg ggaaatggcc
     1141 tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct gctggaacgc
     1201 attaccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac cacgcgcagt
     1261 attcactggt tccaggaacg tggatttacc ccggtggata ttgatttact gcccgagagc
     1321 aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga tttagggtaa
     1381 gcgatgaaaa ttgtcggatg cgacatgcgt aacactcgtg cgtcgcatcc ggcaattacg
     1441 tttattcccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta cgagtggcga
     1501 ttgccgcact taatatacgc tcatcggcat acagcgacag acggcgacgc gcgcgggtca
     1561 ccgcggtgta taccagctct cgcgttacta ccggcgtgcg ttggctcggt aaaatcaacg
     1621 ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac gtagtttcgt
     1681 gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc gcaaaccaga
     1741 cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac aacccaagcg
     1801 cgctgtcatt acgggcaatc attaccggtc gaccttcgta ccaacgagag tgcggatgac
     1861 gatgaatttt gcgcttctgt tgcataaact gctcaattcg ctcattcagt ccagccacgc
     1921 caaacggccc ttcccgcagg gcgcacaaaa gttggtactc attgaacgcc tgaatgatta
     1981 aatccggctc ggcacgcgct tgcagcagat ccagataacg tccataaccc gcaagagctt
     2041 cctcaagcat cgcaatataa tcttctccgc tctgtaaaag ccgtttttcg atatcagtaa
     2101 aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc gccgcagcta
     2161 actgaccaat gccagaatca ctgccgaaac gatagctttt ttgcagcagg cagagactgt
     2221 cgcgcaaaga tgccgcttct gtgccagttc ctgccggaac gtgagttccc gtcaggcggc
     2281 ttagctgccc ggcacgctcg gcggtaaagc cggcattggc ataagcgcag atatcgccca
     2341 gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag atcactcgcg
     2401 catgatcggg caaggcgtcg atcagtctcg acatcatagg cagatcgatc attgacgctt
     2461 catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt aaacgctggc
     2521 tacccggctg cgcacccagc aatcggtgca aagtactggc atcttccgga atgcgtttct
     2581 tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg gttaagcgcg
     2641 cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg tcggccattt
     2701 gaattaacgc tgccagtaac ttcgccacgg tggtcgtttt accggtgcct gggccgccgg
     2761 aaatcaccga gatccgccgc gtcagcgcca ctgccgctgc aactttttgc cagttaattt
     2821 catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca tcaacctcaa
     2881 tggcatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac cacatgcgat
     2941 tcaaataaag acgatcgcca cagaggatca tcggcgtggg ttcatctccc ctgctgaccg
     3001 cttgagaagc cagcaagcat tcttcccaat tttgtagctc accgatttca ctgacacagg
     3061 tcgccaacag cggatgcgac gcctcgttat tttccagtcg tgaaagcggc aaacagacgt
     3121 gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca ggatgttcat
     3181 ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg tgctccactg
     3241 cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac cggcaaacat
     3301 ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc cttgttgcgg
     3361 atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt gacgatcata
     3421 gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat aaagctgata
     3481 ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg tgtaagccga
     3541 actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc cttcgtggcg
     3601 gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact ccagcggcgg
     3661 gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc tggcgatgag
     3721 cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt cggaaagctg
     3781 actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga tccactcggt
     3841 caacactggt tcccactgcg attcaaagcc gccgagttcc agtttttcct gtacccagtt
     3901 cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga atgtccctgg
     3961 tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga cgctgaccac
     4021 gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt gaccacgctg
     4081 ctgcaaaccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt gtagcgtcct
     4141 cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctgtttat cacccgtttg
     4201 tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc gaagccctgc
     4261 cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt ggacgtcggt
     4321 gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac tgcaatgcca
     4381 aaccgaacgt gtcagcgcca cgtaaagcaa acgcagatct tccgccagac gttcggcctc
     4441 cgcgaggtcg acgctttctg gcgcagcatt aagatccaga actgcctcaa acgagtggcg
     4501 atcgtgataa aacgcctgat cctggacgcg gaaattggtg ataaacggca gccagaccaa
     4561 tggatattcc agccctttcg atttgtggat cgtgacaatc tgcaccagat gtttatcact
     4621 ttcgagacgc atttgttggc tggaggcatt actgtctggc tcgaggatat gttgcgataa
     4681 ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgta gcagttcgct
     4741 gatatgcaag atatcggtaa gtcgccgctc accgcctgcc gttgccagca agttttctgc
     4801 aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt tgcgccagat
     4861 ttgccgataa ccatcgaact cttcaactac cgcatcccac gcatgttcgt cattattcag
     4921 cgtttcgata tccagcgcgt tcagccccat cattgacgtt gccagcgcac tacgcagggt
     4981 gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct gcgcttccag
     5041 agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca gcaacgttaa
     5101 ggcatcgcgc acctgggcgg cctcctggcg gctacgcacc agcacactga tgtccgaagc
     5161 acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct gtccggcttg
     5221 tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat agtcgccaac
     5281 gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt cacctttaaa
     5341 tacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg gtatttcgcg
     5401 aaacatgaac gcgtcatcag tctggctgaa aagcttattc acgctgttca ccattcctgg
     5461 tgcggaacgc cagttggtgt ctaaagtgta gtgggcgtga acttcgctac gcgccttcat
     5521 ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt cgccaatcag
     5581 caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt actgctgggg
     5641 gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga tcgccgctgc
     5701 caacacctca ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca tgtcatcaaa
     5761 acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct cagccaatgc
     5821 gcgggtgatc accagatcgc ggatcgacaa tggttctgca agcagttgat cgatcgcctc
     5881 aaacagtgga tgtcgcgggg tttccccccc ggctttcgtg cgatcttcta agaaacgctg
     5941 agagaatttt tccagcgact ccggtaactg ataactgttg gtctcttctt ctgcccaggc
     6001 gctgatcttc tcgatccatt tagcctgatt gctacggtta aacttgcgtc gatcaatacc
     6061 agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct gttttaccgt
     6121 atcaatacgc gccacaattt gcgcgtggcg ggaagccagc gtttcatcat cgggcggcgg
     6181 tgctttgata accggtgctt cgccttgcag ataacgatta atatcgcgca gcaacgcctg
     6241 cggccctttc caggtttcaa agaccaccag ggcaatttca cgcggcagcg ggtagcagtg
     6301 gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt caatcagctg
     6361 ctgctcaaac agcataccgg attcaaaggc attcaggttg agcatgcgct ggcaaaaacc
     6421 gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca accactgcgc
     6481 ggcttgcgct ttatcgtcga tctcttccag caggcgttcg tacagtggat tgtcggtggt
     6541 ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac cacgcaattc
     6601 tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacggtca gcgggcgggg
     6661 aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg caatcgtaaa
     6721 ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg gcaagcgcaa
     6781 aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc attgtttgct
     6841 gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat tcggctttcc
     6901 cgttctggct gccggaaatc tgcgacagaa tagccatgcc ttgcggctcg accaccgcct
     6961 gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc acgattttat
     7021 cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc gatgcttctt
     7081 cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg atttgcgcaa
     7141 actcttctgg cttcatcgcc cgcaattttg cctctgcggt tgggaaaaac gccttgtaac
     7201 gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc atgccccact
     7261 gacgccccac gctcattgga aacgcaaaca cggcatagcc caactgttct tcggtacgca
     7321 actgattgta gaaccacggc tgtacgatct gccccaacag tgagctatag gctgagctgg
     7381 tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg gtgctgttac
     7441 cggctttttc aaagatgacg gattgttttt tatcgaccac gacatctttg ttacgacacc
     7501 actccgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt gttgcctggg
     7561 cttcggtcat gttgccaata accataaact ctggtcgagc ccctgatttt aaggcgtcgc
     7621 gataggccag cacctctttc aacgtaatgg agggcaaaat tttgcgccgt tcatctcgcg
     7681 agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctcg aacgctttgc
     7741 ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca agctgatctt
     7801 ccgtagcggt atagttaaag tagccctcga gcaatgcctg gaacagctgc ggcagacgct
     7861 gggtgtaacc attagcatta accataaggc cgttgttagc gttggtggaa aaacttatgc
     7921 caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga taatcattga
     7981 gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc aaaatcaggc
     8041 tgacatcggc tttgggctcg ctggcaaaat aacggcttgg cgcatacact actcgcagat
     8101 tcgactcatc aacaatcagc tctggatggt cgtatttctt ctctgactta atcagcgaga
     8161 aatcgtcagg aatataaggg ttaagctctg gcaaagagag cgcaatgtcg gcggcttttt
     8221 tctgccagtc ggcgaaagtt tgtgcgctga ttttatcgac ctgatacggc gcatcgacaa
     8281 agtaagccgt tttgttgtgc ggctctttcg ggctgatata ccagatacgc gcattctgcg
     8341 gcgtcatcat cgccagacgt tcctttactg ctttagcatc gtaccgatcg gcaatattga
     8401 ctgcatccag cgtatgctca acaggaacgc gaatcatggt atctgccagc cattcgacgt
     8461 aatccatatc acgggtgatt gacggataac ggaagtcgat atccagcaca ttcgccagtt
     8521 catcgaagta ttgtttatcg atcccttttt cacgtaacaa attgaggtag ctaaaaatag
     8581 ccgccacaac ctgatcgcga ttcgccagac ctttatcggt taaagacgca gagatcgcta
     8641 atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc tcaactaatc
     8701 cctgcttttg cagccagtcg gaaagtgtac ctgggctgcg attaccaatc agataggtaa
     8761 tcaattcatc ggttttgcta cggaatttcg ccgagttgtt atcaatacga aactctacgc
     8821 gcaacacttt acgcggcagc gccgggacgt aatgaatgat aatgcccttt tgcgcgtcgg
     8881 tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg cgaccaaagg
     8941 tgtccgccgc catttttgcc aactccggca gcggtttatt gctgtaaata accgccttca
     9001 tcagattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc accggattac
     9061 caggtttatc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg gggtgtgccg
     9121 ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta cgcgccatgg
     9181 ttaattcagc gttcaccgca ttacgctcac gttcggcata tttcttgtcg agcaaaggtt
     9241 cagcaatagc atcggccagg cggtctaccg caccaggcaa ggcgtcgttc tcaacttcca
     9301 gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg tgcattttga
     9361 gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga ctcatatgtt
     9421 caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca acgggcacca
     9481 ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc accataccgt
     9541 tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg atggtttcct
     9601 gaatcggctg ccatcccgtt tctgcctgac ttaagggtgc ccaaagggca acaaacaaca
     9661 ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa ctttattttc
     9721 tggccagatt caacaggtta acgcaaatca tctgccagaa tttaatcttg tgctgcacga
     9781 gtcagcctat gtctatatat ccatcagtcc gtgactggtg cgcatcataa agtaagcgga
     9841 taggttgcgc aatttttata cagcactcat gactgattaa agcgaaacag cggtaacagg
     9901 aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg ccgccagagc
     9961 ctttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc gtaagcctga
    10021 aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc atcgttttgc
    10081 gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag caatggcgcg
    10141 gacatccctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc ctgttcggct
    10201 gcaagcggcg gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact ttcaccatta
    10261 ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg cgccacactt
    10321 aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa ccagccagtt
    10381 atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg gcgacaggca
    10441 atgactctgt cggcaagctg ctgcatctcc tggcactgtg tttcccagaa aatttcacca
    10501 aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg ttcggcatca
    10561 tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct aagcccttcc
    10621 agaataaatg gctcggtgtc tgggatttcg ctgtcttcag tacggaagtt cacctgcaaa
    10681 cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt ttccagcggc
    10741 acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc tttaccagcc
    10801 tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg ctggtagttt
    10861 tgcggatcaa acggcatccg ggtatggagg caagtaagat gcgcttttac ccttgcctcg
    10921 ctttcatcac agttgagcgc ttcatcgccc ggtagataat gactttgccc gatgtagtcg
    10981 atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat ggaacgcccg
    11041 atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa caggtagcgg
    11101 tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag gtcaaagccc
    11161 aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca aaccactttg
    11221 aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc gagaaaacgc
    11281 tggctgatac gttcttgatc cagacgctgc gccagttcat cacgcaatag tgacagcggc
    11341 accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca ttgttgttcg
    11401 atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt gagcatatcg
    11461 cgacaaaccg gcaaccactc ttctagcgga cgctcctgtg ccagcccgcg acgccagatg
    11521 tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa gccgctcgat
    11581 tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc gtagcccagc
    11641 aacatacgcg tcaggccaaa tcgccaagtg tgttgtccgg tggcggggag ttccagctcg
    11701 cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca ctggcgtaaa
    11761 taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac atccagtaat
    11821 gccagcacgt cttccggcac aaagcggctg tctggcaacg ataacaggct gataaacgct
    11881 tccagtaccg gatgcgactg ccgcgcccga cggtcggaaa tggcgtaagg caggtaacga
    11941 tccgcaggtg cgctaccaaa cacagcctga ataaacggac tgtagctgtc gatatcagcc
    12001 accatcacga tgatgtcgcg cggagtaagt gtcgggtctt cttccagcat cgccagcagg
    12061 cgatcgtgca aaacttcaac ttcacgctgc gggctatggc aaacgtggaa ggtgatactg
    12121 ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc gatgttcaca
    12181 ccagcaacgg cacggttttc cagttccaga atgtcagact gaatgttatg cagcaggtta
    12241 tctggcgtga catcgacaaa ggcgtccagc tcctggctgc tctccaggtc agaaaggaga
    12301 taaatgtagt cgcgcccaag cttaccccat gaagccagca gcgggttgcc gacatcctgt
    12361 tcaccatcgc tgttaaagag ctgcccggca ttttcgctat cacgaaatag cggcaattcg
    12421 cgatcttcaa aactgtgtcg acgctgacgg gtcagcagtt tcgccagata agcaggatct
    12481 ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat ttcaatatgt
    12541 ttacccagcg cctgtagcgc ctggagataa acaggcggta acgcggaaat accgcatata
    12601 aagacgcgcg aaggtagccc cggcgggcag gtcgtcgcgg actccagcgt ttcgataaag
    12661 cgctgataga gattggcgcg gtgccagcgc ggttgcccga gttgatgggt atattccacc
    12721 aacgccttcc acaacggcgc ttgccaggcc tgtgcttctc ccagcccttc aaccaaatgt
    12781 cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc aaacaggtcc
    12841 gccgcttttg aagaaagctg gaacagtttt cgcttgtcgc tatcgtcagt cagataatgc
    12901 cgcaacaggg taaagtcttc gcgctccaac aattgcggca gcagagtcat cagtttccag
    12961 ctcatgctct gtttgttaaa ggcgctctct ttggggattt ccggtaacac ccggacgaac
    13021 atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat accaaacttt
    13081 tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag aatcatctct
    13141 ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa cgcttccagc
    13201 acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta ctgacgattc
    13261 gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag cgtaacgctg
    13321 atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt ggcaggtggc
    13381 gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg gtactggttt
    13441 ctgctggcga gactgttcat taatgttcgc tggatacccg ataacgcagt gacaatcatc
    13501 accatcagca ccatcgcaac cattacctcc ggcaggctaa agccttgttg attcttcagg
    13561 gaagctgaca taatgccccc tctttcagcg gacaaaaatc gctccagccg cgtggcgaaa
    13621 aaacaatttt tccatcaatg acttcgcctg ttcgccacaa cgaaacgcct tcataaccgg
    13681 caatcaataa ggcttcatta tctgccagta aacgcaaaca aacctgggca tcggttccag
    13741 cgtactgcga gcactgaact gctggctgcg tctgccagga gtgcattttt ccccacgcca
    13801 gcgccgactg aacgatggcc tggcggcgca atgactgact ttccatgctc acgcgagagg
    13861 caaaactgcg atcctgctga ctcattcctt gcaatagcaa gctacccaaa accagcagca
    13921 tcaggaccag agccagtgac gaaacacctt tttcgcggtt cacaggttga atcctgtcac
    13981 gctatagctg gcatccacca cggtttgcgg ttcagactta ctggcagcac gcatattaac
    14041 cgtcaacacc ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag tgtcgataat
    14101 gatggcatcc ggattagtga ctttatccca gcccttacct tcacaggatg tcgcaccgcg
    14161 tagcgtttcc agcacatgct ccttcagacg aaatccaatc tggtcggact cttttaccgg
    14221 ttcacgatcc cagataccgt tactgttcgc atcccactgc acaatgatac agtcaccctg
    14281 tccgacaatt tccagccctt cgccggtaca gctgccatga caataacccg ccctctggag
    14341 atgcttcgcg acggtaaata cccgcagcca gatttcatct tccagcgcca gcttacgggt
    14401 gctcgttaaa ctttcacgct gtaacgcagg cagaaagcgt gcagccccca gcaacaatac
    14461 gctactgatc gccatagcaa tcaacacttc cagcagagaa aaaccttgct cttttacagg
    14521 catccttctg tttctccttg ctgacaaagc cggagtcttc cccacggcga aaccaccagc
    14581 caccactcgc ccgttgagtt tttgaagcga atatgcccgg cccatgcggt attgcgcagg
    14641 ccaaagaaag caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca gcgtggcaca
    14701 aagaccaatg gtgaactgcc atgacaggta ttggccccag cagcggaact cacaaggcac
    14761 cataacgtcc cctccctgat aacgctgata ctgtggtcgc ggttatgcca gttggcatct
    14821 tcacgtaaat agagcaaata gtcccgcgcc tggctggcgg tttgccatag ccgttgcgac
    14881 tgctgccagt attgccagcc atagagtcca cttgcgctta gcatgaccaa aatcagcatc
    14941 gcgaccagcg tttcaatcag cgtataacca cgttgtgttt tcatgccggc agtatggagc
    15001 gaggagaaaa aaagacgagg gccagtttct atttcttcgg cgcatcttcc ggactattta
    15061 cgccgttgca ggacgttgca aaatttcggg aaggcgtctc gaagaattta acggagggta
    15121 aaaaaaaccg acgcacactg gcgtcggctc tggcaggatg tttcgtaatt agatagccac
    15181 cggcgcttta atgcccggat gtggatcgta gccttcaatc tcaaagtctt cgaaacggta
    15241 gtcgaagatg gattcgggtt tacgtttgat aatcaacttc ggcagcggac gcggttcgcg
    15301 gcttaattgc agatgagttt gatccatatg attgctgtac aggtgcgtgt cgccccccgt
    15361 ccagacaaaa tcgcccactt ccagatcaca ctgctgcgcc atcatatgca ccagtaacgc
    15421 gtagctggca atgttaaacg gcaggccgag gaaaacgtca caggagcgct gataaagctg
    15481 gcaagagagt ttgccgtctg ccacatagaa ctggaagaac gcatggcacg gtgccagcgc
    15541 cattttatcc agttcgccta cgttccacgc tgaaacaata atgcggcgcg aatccgggtc
    15601 gtttttcagc tggttcagta ccgtagtgat ctggtcaata tgacgaccat ccggcgttgg
    15661 ccaggcgcgc cactgtttac catacactgg cccgaggtcg ccgttttcat cggcccattc
    15721 gtcccagatg gtgacattgt tttcgtgtag ataagcaatg ttagtgtcgc cctgcagaaa
    15781 ccacagcagt tcatggatga tggaacgcag gtggcaacgt ttagttgtca ccagcgggaa
    15841 tccatcctgc aggttaaaac gcatctgatg accaaaaatg gaaagcgttc cggttccggt
    15901 acggtcgttt ttctgtgtgc cttcgtcgag cactttttgc atcagttcta aatactgttt
    15961 catggttcct caggaaacgt gttgctgtgg gctgcgacga tatgcccaga ccatcatgat
    16021 cacacccgcg acaatcatcg ggatggaaag aatttgtccc atgctgatgt actgcaccca
    16081 ggcaccagta aactgcgcgt cgggctggcg gaaaaactca acaatgatgc gaaacgcgcc
    16141 gtaaccaatc aggaacaaac ctgagacagc tcccattggg cgcggtttac gaatatacag
    16201 gttgaggata ataaacagca ctacaccttc cagcaacagc tcgtaaagct gtgatgggtg
    16261 gcgcggcagc acaccgtaag tatcgaaaat ggattgccac tgcgggttgg tttgcagcag
    16321 caaaatatct tctgtacggg agccagggaa cagcatggca aacgggaagt tcgggtcaac
    16381 gcggccccac aattcaccgt taataaagtt gcccagacgc ccggcaccaa gaccaaacgg
    16441 aatgagtggt gcgataaaat cagagacctg gaagaaggaa cgtttagtac ggcgggcgaa
    16501 gataatcatc accacgataa cgccaatcag gccgccgtgg aaagacatgc cgccgtccca
    16561 gacacggaac agatacagcg gatcggccat aaactgcggg aaattgtaga acagaacata
    16621 accaatacgt ccaccgagga agacgccgag gaagcccgca tagagtaagt tttcaacttc
    16681 atttttggtc cagccactgc ccggacgatt cgcccgtcgt gttgccagcc acattgcaaa
    16741 aatgaaaccc accagataca tcaggccgta ccagtgaagc gccacgggtc ctattgagaa
    16801 aatgaccgga tcaaactccg gaaaatgcag atagctactg gtcatctgtc accacaagtt
    16861 cttgttattt cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt tcaggcgctc
    16921 caaaggtgcg aataatagca caaggggacc tggctggttg ccggataccg ttaaaagata
    16981 tgtatatgat ccgcgctata accctccgcg aatcagcccg cccatgccgc gacgctccat
    17041 aaaggctgca acctgatggc gaacttcggt cgccagttgc gcttccagac tacgctgcgc
    17101 aagattttct gcgtcggcat aatcaatgcg ccgcagcagg tattttaccc gcgctacaga
    17161 acgtccgttc atagacagat ggcgataccc aagcccaatg aggattgcca cgcacatggg
    17221 atcgcccgcc atttcaccgc acaaacggag atcgattcca tgtatttccg cttcccgggc
    17281 gatcatcgcc agagctcgta acattgcagg atgaagactg tcataaatgt tcgccacccg
    17341 ggtattgttg cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc caacagagat
    17401 gaaatcgacc cgctttgcca gatgcggcag cataaatacc attgacggca cttccagcat
    17461 gatgccgata cgtggtttgg gaatttcgta accgatcatc tcctcgactt cacgtccggc
    17521 acgttcaatc aggcggcgtg cttcatcaac ttcatcgagg cttgtgacca tcggcaacag
    17581 aatattcagg ttgcccgtag cggcattagc acgcagcatc gcccgcacct ggatcaagaa
    17641 gatctccggc tgatcgagcg taatgcgaat cccacgccaa cccaggcatg gattctcttc
    17701 gctgatcggc atgtaaggca gctgcttatc tgctccgaca tccagcgtac gcaaggtgac
    17761 gggtttatca ttgaacattt gcagcatccc ctgatactgc gccacctgtt cttcttccga
    17821 cgggaaacca ctttgcagca tgaatgggat ttcagtgcga taaagaccaa tgccatcaat
    17881 acggctgccc agtttttctt catgttccgg gcttaaacca gcattgagca tgacttttat
    17941 acgctcaccg ctttttaact gcgcgggtaa attgacgtca tcttccgcca gacggctaag
    18001 ctcaatctct tcactaatta gccgctgata ttcttgcagc agtaccggct ccggatcgac
    18061 cagcaattca ccgcgatagc catcaacgat cagcgtccta cgatgcagca ccgaaggctg
    18121 aatatccgcg cccatcacgg tagggatccc cagcgcacgt accatgatcg cagcatggga
    18181 gttggctgcg ccatctcgca cgacaacacc gactaagcga tcctggggca gctcagcaag
    18241 agttgtcgct gacagttcat ctgccaccag aatgaaacgt tccggccagg cgttcggccc
    18301 ttgattagcg tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta aatcgccagc
    18361 ccgctctttg agatagttat cgcttagcgc ggcaaactgt tcggcaaatt tttcaatgac
    18421 cgtttttacc gcccactctg ccaccgagcc tttatcaacc tcggcaaaca attcgcgacg
    18481 cagccgggtg tccgaaagca ggtgcgagta aagatcgaaa atagccgccg tttctttttg
    18541 tgctccggcg gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt cttccagcgc
    18601 cccggtcaat cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat acacctgttc
    18661 cattaaaggt aacgtggcat cctgccagcc ttcggcaatc gccacaccag gtgctgccgg
    18721 taatgcgcgg attcgcgtct ggcgatattg cccaaacaag gcagtcaact gcgactgaga
    18781 aagaatagct gccatctggg tggcaagcgt caccaggaag gattcttcac tttcgtcata
    18841 ctggcgcaac tctcgttgct gtaccaccag tacaccaagc aactggcgac gttgaataat
    18901 tggtacgcct aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt atttgaagct
    18961 ggggtgcttt tgcgcatctg caaggtttat cggttccgcc agcctgccaa ccaggccgac
    19021 gatcccttca tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca gcccccgggt
    19081 cgccatcagg tagtaacaac gtcgatcatg atcggccagg tagaccgaac agacctcggt
    19141 atccatcgca agacagatgt cggtaaccag aatatttaac gcctcattca ggcgtggtgc
    19201 gctggctacc ttttcgacta tttcgcgcag gcgagtgagc ataattggcg tgacttaacc
    19261 tcttttacgt cgataagcag atgcgttttg tggttttggc gtattttcct gcagtgacat
    19321 caccacactc gcgaactctt tcattaccct acggtagaca tcacgtttaa atgacaccac
    19381 ctgtctgacc ggataccagt aacttaccca tcgccagccg tcaaactctg gtgtactgct
    19441 ggtttgcata ttgatttctg catcgccgct caccagctgc aagagaaacc atttttgttt
    19501 ttggccgata caaaccggct tcgtgtccca acgcaccaaa cgtttcggta atttgtagcg
    19561 caaccagtta cgcgttgaag caaggattcg aacgtctttg cggcttaatc ctacttcttc
    19621 aaacaattca cggtacatcg cctgctctgc ggattctccg gggttgattc cgccttgcgg
    19681 aaattgccag gagtgctgac caaatcgccg ggcccacatt acctgcccct ggcgattaca
    19741 aatcacgata cctacgtttg ggcggtagcc atcgtcatca atcaccggac tacctcaaaa
    19801 taaagcttta tatacgaatg attgtttcat actccaggaa gacggtaaac cactctctgc
    19861 agggcattac acactaataa caattgaata actcacagtt atgtgcagag ttataaacag
    19921 aggaaggggt ggatagccgt ttttattcac cttttctgtg gatagagttg tgaagaacta
    19981 cggaattact acgggaaaac ccggagcatt ctgaataagc ccgatataga cattttaaaa
    20041 tattcattat tagtcaatgc attacatgta atttgagtaa tgaaaaagag taattcgtga
    20101 cccaggtcac acctctcatt tacgggttgg cgaaagatca accaatgccg tatttatcca
    20161 cagaatgtgc cactaagtta agcactgaac cactaaaaac tggagtttcg tcgcacgtca
    20221 aggctgtaaa tggaaacagt agtggaggtt tttcacagtt atcccagctt tctgtggata
    20281 acatggtgta agatcctgtt tattttcagt gaccagattt ggaaaacccg ttgcagtgtt
    20341 gcgcaactcg attaccggca gcctaaaaag caatataaat cagagaattg aacaacacat
    20401 gtggaaaaag ttacactgcg aatattcggc acataattgc tgtttgtttt ttaaccaagg
    20461 tatcatgaca tgtcccaacc tcgcccactg ctctctcctc ccgaaactga agaacagttg
    20521 ttagcgcaag cacagcaact ttctggttat acattgggag aactggcggc acttgccggg
    20581 ctggttacgc cagagaattt aaaacgcgat aagggctgga ttggcgtgtt actggagatc
    20641 tggctaggtg ccagcgcagg gagtaaacct gagcaagatt ttgctgctct gggcgtggaa
    20701 cttaaaacta tccctgtgga tagtcttggt cgtccgctgg aaacaacatt cgtttgtgtt
    20761 gccccgttaa cgggtaatag cggggtgaca tgggaaacca gccatgtgcg ccacaagctc
    20821 aaacgcgtac tgtggatacc ggttgaaggc gagcgcagca tcccgctggc gcagcgtcgc
    20881 gtaggatcac cgttgctgtg gagcccgaat gaagaggaag accgacagct acgcgaagac
    20941 tgggaagaat taatggatat gattgttctc ggtcaggttg agcggatcac cgctcgtcac
    21001 ggggaatatt tacagataag accgaaagca gcgaatgcga aagcgcttac cgaagccatt
    21061 ggtgcccggg gcgaacggat tctgacgctg ccacgcggct tttatttgaa gaagaatttc
    21121 accagtgcac tactggcccg tcattttctg atccagtagc catcgctttg acctgccgct
    21181 ttccgggcat ataattaccg cttcattttt ttggcagggc tttttagatg ttatttgcat
    21241 ggataaccga tcctaacgcc tggcttgcgc tcggtacgct gacgctgctg gagatcgttc
    21301 ttgggatcga caatattatt ttcctttctc tggtggtggc aaagcttccc acagcacaac
    21361 gtgctcatgc gcgccgtctg gggttggcgg gagccatggt tatgcgtctg gcgctgctgg
    21421 catcaatcgc ctgggttacg cgcctgacaa atccgctttt tacaatattc agtcaggaaa
    21481 tttctgcccg tgatttgatt ctgcttctgg gtggcttgtt ccttatctgg aaagccagca
    21541 aggaaatcca cgaatccatt gaaggtgaag aagaagggct gaaaacacgc gtttcatcat
    21601 tcctcggcgc tatcgtgcag attatgctgc tggatattat ctttagcctc gactcggtga
    21661 ttacggctgt gggtctgtca gatcacctgt ttattatgat ggcagccgtg gtgattgccg
    21721 taggcgtgat gatgttcgcc gcgcgctcga ttggtgattt tgtcgaacgc catccttcgg
    21781 taaaaatgct ggcgctctct ttcctgattc tggtgggctt taccctgatt ctggaaagtt
    21841 tcgacatcca cgtaccgaaa ggttacatct acttcgcgat gttcttctct attgcggttg
    21901 aaagcctcaa cttgattcgc aacaaaaaga atccgctctg atactccgta cgctctcctg
    21961 cgggagggcg tactcactcc ttttaccctc acagtttaag attttcctgc tttcaagata
    22021 tataacgtcg gtttataaac agactattat cataggtagg ccaaatgagg ataaccggat
    22081 gaaaaaatgg gcagtaataa tttctgcagt cggactggcg tttgctgttt ccgggtgttc
    22141 cagtgattac gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg gaaaacctga
    22201 aattgatgat gataccgggc tggtgagtta tcacgatcag caaggtaacg cgatgcaaat
    22261 taaccgtgat gatgtttcgc aaattattga acgttaacaa ataaggtcag catccggctg
    22321 gccttaagat ttttctcttt cccttttccc ttccctctgc catttttata ttccttatgt
    22381 cgtgattata aaaaggaaac ggctatgcaa tatcaccgta taccccacag ttcgctggaa
    22441 gtcagcacgc ttgggcttgg cacgatgacg tttggtgaac agaacagcga agccgacgcc
    22501 cacgcacaac tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt tgccgaaatg
    22561 tacccagtac ctccgcgccc cgaaacgcaa gggttaaccg aaacctacgt cggcaactgg
    22621 ctggcgaaac atggcagccg cgaaaagtta attatcgcct ccaaagtgag cggaccgtcg
    22681 cgcaataatg acaagggcat ccgcccggat caggcgctgg atcggaagaa tatccgcgaa
    22741 gcgctgcatg acagcctcaa gcgcctacag actgattacc tcgatcttta tcaggtgcac
    22801 tggccgcagc gcccgaccaa ctgcttcggc aaactcggtt atagctggac ggattctgcg
    22861 cctgcggttt cgctgctgga tacgctggac gcactggcag agtaccaacg cgcgggaaaa
    22921 attcgttata tcggcgtgtc gaacgaaact gcatttggcg taatgcgcta cctgcatctg
    22981 gcggacaaac acgatctgcc gcgtattgtc accattcaga acccttacag tctgttaaac
    23041 cgcagttttg aagtaggtct ggcagaagtc agccagtatg aaggggtcga actgctggcc
    23101 tattcgtgcc tgggtttcgg cacgctgacc gggaaatatc tcaatggtgc aaaacccgct
    23161 ggcgcacgta atacgctctt tagtcggttc acccgctata gcggtgagca aacgcaaaaa
    23221 gccgtcgcgg cgtatgttga tatcgccaga cgtcatggcc tggaccctgc tcagatggcg
    23281 ctcgcgtttg tacgccgtca accgtttgtt gccagcactc tgctgggcgc aaccacgatg
    23341 gatcagctga aaactaacat cgaaagtttg catctggagt taagcgaaga cgtattagct
    23401 gaaattgaag cggtgcatca ggtttatact tatccggcac cataagatta acgccggtta
    23461 aaaccggcgt taaatattaa tgacggcgct gccagatcca cagcgccgtt attgccagcg
    23521 caaacagcgc accgaagcca atgccaatgg gcacgaccgg gatgcctatc attaccgcca
    23581 gcgagtaaat gcccagcatc aacaacatgg cgctgttttc gccaaggttt tgtactgcaa
    23641 tcgcattccc cgccccgacg ctttttttac cccgctcctg tagcaacgca ttgagcggaa
    23701 cgacaaaaaa gccccccatc acgccaatca gcatcaacaa ggcataggct ggcagcagct
    23761 cgtgttgcag ggaaaaaatc agtaccacca cgccaatcaa aatcccggct ggcatacagc
    23821 gtgacacggt ttccagcgta actaacttcg ctgccgcacc tgcgccaacc acgataccaa
    23881 tcgctaccat cgcgttgaga taggtgggcg tagcgttatc ggtaatgccc agcgccaccg
    23941 gtacccacag caccaacagg aaacgcagcg tgacacccgc tccccagaat aaactggtgc
    24001 ccaccagcga aaaacgcgtt tcaccattgc gccatagcga ggtgcaggca ttcaggaaac
    24061 tgcgggtcat gttgatgaga ttccaggact gccccggacg cgccgccgcc agtttgggaa
    24121 tgtagatatt ggcaacgacc gcaccaccgt aggccagtgc gcatgcggcc agggcgacga
    24181 ggacatgcca gtcagccagc acaccaccgg ctacggaacc gagcaaaatc gccgctatgg
    24241 tagaagcttc cattaaaccg ttagctttca ctaacttact acccgtggtt aattcgccga
    24301 gaataccgta tttcgccggt gaataggctg cagcaccaac acccaccagc gtatagccga
    24361 gaaacggatt gataccaaag cagatactgg ctgcgcccag cagcttcagg ccgttggcaa
    24421 acatcatcac ccggcctttg gcgaagctat ccgccacctg cccgacaaac ggcgcaaaaa
    24481 gaatgtaagc acctacaaac accatttgca ggatgggctg gctccactcc ggatagaact
    24541 gcgctttcag taacgccaaa gtggcaaaca gtagggcatt atcgccaaac gcagagagaa
    24601 actgcgccac gataaccgct ttcatcccct tcgaccacaa cgaagtgtta gtgtgcactg
    24661 actcactcat cgtgttgttc cgcttcgtct acccagcttt tcaacgtgac aaagtcaggt
    24721 ttgccgctgc caagtaatgg catctgtttc agatagagaa tatcgcgcgg tacagcaagc
    24781 tccggcacgc cgtgctcgcg ggcatactgt tgcaacttat cgcgcgtcag ttcgttatct
    24841 gtggtgaaaa gcaccagtgc ctcgcctttg ctggcatcgc tcttaatcgc agtggcatgg
    24901 actttatctg gcgaaacacc aagtgccagt tgttccacca tttccagcga caccatttcg
    24961 cctgcaattt tggcaaagcg ttttgcgcgg ccctgaatct gcacaaagcc cagctcgtca
    25021 aaacgcacaa tatcgccagt gtcataccag ccgcgctcca tttcgccgcg aacattctcg
    25081 gcggtgggca cttccagtac acctggcttc tccacccgca gatagccgtt cattatgttc
    25141 ggccctttca gttgcaggcg tccgccctct tcgataccag ggaccgacaa caggcgcgca
    25201 tccattcctg gtagaatacg ccctaccgta ccgggtttcg ccgccatcgg tacgttgata
    25261 gaaacgacag gcgcgcattc ggtcacaccg tagccttcaa ggatgcgcag gccaaattta
    25321 tcctgccaaa gctgtttggt actttcttgt agtttttctg cgcctgccac cacatagcgt
    25381 agacgataga agtcatacgg gttggcgaaa cgcgcgtagt gaccgaggaa agtcgaagtg
    25441 ccgaacaaca cggtgcaact gcggtcatac accaactccg gcacaatacg gtaatgcagc
    25501 gggcttggat aaagaaacac ttctgcacct gtgagcagtg gcgtaaacag gcctaccgtc
    25561 agcccaaagg agtgaaacag cggtaacgcc gacataaagc gatcgttggt ggtgaagtcg
    25621 gcaatcgttt taatctgctc gacattcgcc agaatgcttt tatggctatg gacgacgcct
    25681 ttcggatggc cttcagaacc ggaggtaaaa aggatcagcg cctcttcttc cggctgctgt
    25741 ttaacctgtg ccagacgcgg catcagcaaa tgagcgaaga tccatacttt gtcggcagtg
    25801 gtgacatctg cttttaaatc ttccagatag acccagcgca cctgagtaag ttgctccggc
    25861 agatgccaga gtttgccttt atcgagaaac tggcgggaag tgaagatggt tttgatttca
    25921 gccgccgtaa tagcactggt cagccctttt accccggcag tgtagttcat cattgcggga
    25981 atgcggcgac gggcgatggc cccaaaaatc actgccgcac tgatgcctgc attgggcagc
    26041 attaagccga tgcgttcgcc ttcaacactg tatttttcaa ggatgcgtcc aacaaacagc
    26101 gttttcgtaa gcaatttgcg ataggagtct ggggtaaagt tgacgtcttc gacacatttc
    26161 ttcccggctc cgaagcggta cattgcactc agtaaagatt cgtacagcgt ttcacgcggg
    26221 cgcaccgcca ttcgcgcttc catcattatt tgatgcagca tttcgccagc gattttgcga
    26281 cggtcacggg cacgcggcgc atccggcatc tccacctgcg ttggtggcaa aatatgcaga
    26341 gtaatttgcg ggaacaagcg acgtttaacc agacctttca ggcggctgaa gtgcgtgagt
    26401 tccgcccctt caatacgcac aggaataacc gttgcaccag acttcgccgc gacaaaaccc
    26461 gcgccatcgt agattttcat cagcgagcct gtagtggtga tgcgtccttc agggaaaatc
    26521 accactggtc ggccctgttc caccagacgt accagatgtt taatagccat aggttgcgtc
    26581 gggtcgagag gaacaaagtc gataaatgat ttcagccaac gcatatacca ctgttggctt
    26641 attgaggtgt aaacggcaaa cactggacgc acaggtaaaa acagtccaag caaaatgcca
    26701 tcaataaaag agacgtgatt aggcgtaatt agaacgcgct cgcccttcag tgcctgggtg
    26761 tcacccgtaa cgcgaacgcg atacaaaaca cggcacaaat ttcgaaaaaa gctaaaaagc
    26821 attccaactc cctttgctct gattcagtaa aagcgaatgg agggagatta cacgagataa
    26881 agaacgcgag cgacagtaaa ttaggtgcga aaaaaaacct gcgcatccgc gcaggttggt
    26941 gcaagagaca gggtacgaag agcgtaccga ataatctcac caatcaatac ctctgggatc
    27001 ttgattgtgg tctgcacgac gactcttcgc cagcgagaaa acgcaaagga atgaagggaa
    27061 atgcaacgag gtgtgtaaat tgtcggttac tgttacagat tgatgaccgg caaaaaaaaa
    27121 cctgcgcatc tgcgcaggct ggtgtaattc atgtgctcaa cccgaagttg acttcaccta
    27181 tcaatacctc tgggatcacc actttagcaa cctgaagcca aacgccacca gcggtcaatc
    27241 gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc tctttttttg tcttacttat
    27301 ttgcgtttgg ctcacattcc cacgatgaaa acacgccacc ccttgaacca acgggcgttt
    27361 tccgtaacac tgaaagaatg taagcgttta cccactaagg tattttcatg gcgaccataa
    27421 aggatgtagc ccgactggca ggcgtttcag tcgccaccgt ttcccgcgtc attaataatt
    27481 cacctaaagc cagtgaagct tcccggctgg ctgtgcatag tgcaatggag tctcttagct
    27541 atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac cactgaaacg gtcggtctga
    27601 tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt gaaagcggtc gaacaggtgg
    27661 cttatcacac cagtaatttc ttattgattg gcaacggtta ccacaacgaa caaaaagagc
    27721 gtcaggctat tgagcaactg atccgccatc gctgtgctgc gttggtcgtc catgccaaaa
    27781 tgatcccgga tgccgattta gcctcattaa tgaaacaaat gcccggtatg gtgctgatca
    27841 accgtatcct gcctggcttt gaaaaccgtt gtattgctct tgacgatcgt tacggtgcct
    27901 ggctggcaac gcgtcattta attcagcaag gtcatacccg cattggttat ctgtgctcta
    27961 accactctat ttctgacgcc gaagatcgtc tgcaagggta ttacgatgcc cttgctgaaa
    28021 gtggtattgc ggccaatgac cggctggtga catttggcga accagacgaa agcggcggcg
    28081 aacaggcaat gaccgagctt ttgggacgag gaagaaattt cactgcggta gcctgttata
    28141 acgattcaat ggcggcgggt gcgatgggcg ttctcaatga taatggtatt gatgtaccgg
    28201 gtgagatttc gttaattggc tttgatgatg tgctggtgtc acgctatgtg cgtccgcgcc
    28261 tgaccaccgt gcgttaccca atcgtgacga tggcgaccca ggctgccgaa ctggctttgg
    28321 cgctggcgga taatcgccct ctcccggaaa tcactaatgt ctttagtccg acgctggtac
    28381 gtcgtcattc agtgtcaact ccgtcgctgg aggcaagtca tcatgcaacc agcgactaac
    28441 cgcagttaaa gcaattccag cgccagtaat tcttcgatgg tctggcgacg gcgaatcaac
    28501 cgcgcctgac cattatcaaa cagaacctct ggtaacagcg gacggctatt gtagttggat
    28561 gacattgatg cgccatatgc ccctgtatca tgcagtacca gataatcacc tgccttcact
    28621 tccggcaagg cgcgggtttc aacatttccc ccttcctgct gggtaaagac atcgcccgat
    28681 tcacataacg gtccggcgac gacggtttcc accgttggcg cgtgttccag agaacgacca
    28741 tcagctgcca gggcactgat atggtggtaa ctaccgtaca ttgccgggcg catcagatcg
    28801 ttgaacccag catcaaccag cacaaagtgg cggctaccca tttgtttgac gctccgcacc
    28861 tgagtaatta atacgccaga ctgcgctacc aggaagcgac ccggttcaat ttccagtttc
    28921 acagggtggc ccaaatggcg ggcgatttgc tcacgcgcgg cattccacag accataataa
    28981 tgttcggtat caaccgcctc ttcaccctgt tgataaggaa cagaaagccc accgcccgca
    29041 gaaatagcct gtaaatcctg accgaattcg atgacctgac gcaccatagc accacacacc
    29101 tgttccagat gggcataatc aacgccagaa ccaatgtgca tgtgaatgcc gaccagctgc
    29161 agatgatgac gttgtatcac gtccagtgcg gcgggcagat cggtgtacca gataccgtgc
    29221 ttgctgtttt cgccaccggt attggttttt tggctatgtc cgtgaccaaa ccccggatta
    29281 acgcgcagcc atacccgatg ccctggcgaa acctggccca gttggtcgag catatcaaca
    29341 gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt caagcgtcgc ctgatcgata
    29401 acatctgccg taaaaacaat atcatcgggg tgcgtttgcg gattgtaacc cgccgccaac
    29461 gcacgctcta tttcgcctaa cgagacggaa tccactttca cgccctgctc acgcattaag
    29521 cgcaaaatat gaatattgga acaggctttc tgtgcaaagc gcaccacatc aaactgtttc
    29581 agcgctgcaa tctgccgacg aataatttgc gcatcgtaga cccacaccgg gcagccaaat
    29641 tcagcgggca aacgcagcag attttcggcg gtgagatcgg tatcggtgct gaacagtgaa
    29701 tgtggcataa caaactccag ataagtgctt ttttatgatt acgccacatc ataaaaagaa
    29761 taaaaaatat cgatttatgt cgagtttatg caaaaatgat atggattacc ggattgcgag
    29821 agagcgctaa tggccgccgt taacttacgt catattgaaa tttttcatgc ggtaatgacc
    29881 gccggaagcc tgactgaggc ggcacacctg ctacacacct cacagccaac cgtcagccgc
    29941 gaactggcgc gctttgagaa ggtgatcggg ctgaaattgt ttgagcgcgt acgtgggcga
    30001 ttacatccta ccgtgcaagg actgcgtctg tttgaagaag tgcaacgatc ttggtacgga
    30061 ctggatcgca ttgtcagcgc cgcagaaagt ctgcgcgagt ttcgccaggg agaactgtct
    30121 attgcctgcc tgccggtctt ttcgcaatct tttttaccgc agctcctgca accctttctg
    30181 gcacgttatc ccgatgtcag cttaaatatc gtgccccagg aatcaccgct acttgaagag
    30241 tggctctcgg cccagcgtca tgatttagga ctcactgaaa cgctccatac gcctgcggga
    30301 acagaacgta ccgaattact ctctttagat gaagtgtgtg tgttacctcc gggtcatccg
    30361 ctggcggtaa aaaaggtatt aacgccggat gattttcagg gtgagaacta catcagcctt
    30421 tcccgtactg acagctatcg ccagttgctg gatcagctat ttactgaaca tcaggttaaa
    30481 cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtct gcgcaatggt acgggcgggg
    30541 gtaggtgttt cggtggttaa cccgctcacc gcactggatt atgcggcaag cggtttagtg
    30601 gtgcggcggt tcagtattgc ggttccgttc accgtcagcc tgatccgccc cctgcaccgc
    30661 ccgtcatcag cgctggtgca ggcgtttagt gagcatttac aagcggggtt accgaaactg
    30721 gtcacttctc ttgacgctat tttgtcgtca gctacgacag cataaaagcg acagcatcct
    30781 cggcatggat cgccgcggta tcaaacacag gcaaaacact gcgctcttct ggcaccagta
    30841 aaccaatttc tgtgcagcca aaaatgacgc cctgtgcgcc ctgttctgca aggcgagcaa
    30901 tcacttgcgc ataataagcg cgtgacgctt cggtaaattg ccccagacac agttcttcaa
    30961 aaataatctg attaattttc gcccgttcat ccgcttcagg aataagacaa ttgatggaaa
    31021 attgttccgt cagccgcccg cgataaaaat cctgttccat ggtgtaacgc gtacccagca
    31081 gcgccacacg agtcattccg gccccggtaa gtgcacgtcc ggtggcatcc gcaatgtgta
    31141 agaaaggcag agtgcaacgt gactcaatgg catccgccac tttatgcatc gtattggtac
    31201 ataacacaat accttctgcg cccgcccgct gtaagccaag cgccgcctca gccagaatgt
    31261 ccccggtttt atcccattcc ccgcgacgct ggcactcttc tatttcatga aaatcgacgc
    31321 tatgtagcag cacttgcgca gagtgaagcc caccaagccg ctgtttaatg ccttcattta
    31381 tcagacgata gtaaggaatg gtggattccc agctcattcc tcccagcaaa ccaattgttt
    31441 tcattcctct ctcctgatgt gtgttaccgc aacaaggctg aataaggaac gagacaaacg
    31501 cctcaacggc caggtgcccg atctctatta acgaaaaaag ggccggatgt acagcacatc
    31561 cggcccgtga aatcagacgc cgatatttct caacttctcg cctgccatca gtttgcgttc
    31621 gatatgttcc agcgtgacat ttttggtttc cggaatgagc cagaaagtga tgcccacaaa
    31681 cgcaatgttc agcgcagtgt agagccagaa cgtaccggca gcgccaatgc tatcaagcag
    31741 tgtcaggaag gtcgcgccga taatcatatt cgacacccag tttgtcgtcg tcgaacaggt
    31801 aataccgaaa tcgcggcatt tcagcggctg aatttcagag cacaggatcc acaccactgg
    31861 cgcggcgctc atcgcataac cggcaataca catcatcgtc atgccaacag agagccagga
    31921 caagccactg gaagccgtac cgttatcaaa ctgcatcagg caatagccca gcaccagagt
    31981 gcctaacgcc atcacgctga aaccaatttt cagagccggt ttacgacctg ctttatctac
    32041 cgtaaagacc gcaatgaagg tcgcgaacat aaaggtcagt cccacgacca gagtcgcaat
    32101 catctgttgt tctgtggtcg taaagcccgc cattttgaag atacgcggcg catagtacat
    32161 gatgatgttc ataccggtaa actgctgcat cgcctgcaac aacataccga gaaacacagc
    32221 acgacggacg ttacggttga tcttaaacag tgcccaaccg ccttgtttta acttcaggct
    32281 ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac gtatcgcgca gcatacgcaa
    32341 cacttcttcc gcctcaatat gacgcccctt ttccgccagc cagcgcgggc tatttggcag
    32401 gaagaccacc agaataatca gcaggactgc tggtaaagca agaaccccca acattgcgcg
    32461 ccagttaccg ctataactga acgctgtatc ggataaaaac gccagcacga tgccgagtgt
    32521 gaccatcaac tggtacatac tgatcatctt accgcgaacg ttttcacttg ccatttcaga
    32581 aagatacaga ggagcggtgt aagacgcgat cccgacagca atgcccagca ccacccgagc
    32641 ggcgattaac atctctacgc tggtcgcaaa agcggaccct atagaaccga gtacaaacag
    32701 gatggccccc gccatcaggc tgtatttacg acccaggcgg aacgacagcc aaccattaaa
    32761 cagcgcacca attgctgcgc cgagcatcat gctactaacc acccattcct gcaaacgact
    32821 ggtcagcaca aagtgatcgg taatgaacgg caacgctccg gcgattacgc cgatatcaag
    32881 accaaataac aatcctgcga ccgcagcagc taccgaaaca aacatattca tacgccgcgt
    32941 atcccgcaaa gaacgtggcg ttaaagcaga ttccgtattg atagtaacca ttttttcctg
    33001 ccagcagaga gtaagacata gtgaaaaaat acgtgaacaa ctcacgcagg tgtcaggtcg
    33061 gaaacagcat aaatatggat taaattgctg cg
//
LOCUS       DAZFRN010000041        32496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFRN010000041 DAZFRN010000000
VERSION     DAZFRN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.041"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1299
                     /locus_tag="ACHKUB_001872"
     CDS             <1..1299
                     /locus_tag="ACHKUB_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6654898.1"
                     /translation="FLFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTY
                     SGSQQNTGASDTTAQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGIGDP
                     NDPKWVKGIQTRDSVNQNQHESERNYQKAEQNSPNALQNEPETKQPESVAQQEVEKVC
                     TACGQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQ
                     HHDSDNETGETADHSFKVNGHPEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFD
                     PQTGDMGPEFSKTIDLETAGGVIDRNTIKWWLKQSREAQSAIMTDEIPLDDALLQLRE
                     FIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDF
                     DARTAIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADS"
     gene            1372..1623
                     /locus_tag="ACHKUB_001873"
     CDS             1372..1623
                     /locus_tag="ACHKUB_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6654899.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            1658..2938
                     /locus_tag="ACHKUB_001874"
     CDS             1658..2938
                     /locus_tag="ACHKUB_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6654900.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRSIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(3126..4145)
                     /gene="rspB"
                     /locus_tag="ACHKUB_001875"
     CDS             complement(3126..4145)
                     /gene="rspB"
                     /locus_tag="ACHKUB_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO6654901.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(4157..5371)
                     /gene="rspA"
                     /locus_tag="ACHKUB_001876"
     CDS             complement(4157..5371)
                     /gene="rspA"
                     /locus_tag="ACHKUB_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO6654902.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(5577..5903)
                     /gene="ynfA"
                     /locus_tag="ACHKUB_001877"
     CDS             complement(5577..5903)
                     /gene="ynfA"
                     /locus_tag="ACHKUB_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO6654903.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            6038..6379
                     /gene="ynfB"
                     /locus_tag="ACHKUB_001878"
     CDS             6038..6379
                     /gene="ynfB"
                     /locus_tag="ACHKUB_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO6654904.1"
                     /translation="MKITLSKRIGLLAILLSCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            6414..6974
                     /gene="speG"
                     /locus_tag="ACHKUB_001879"
     CDS             6414..6974
                     /gene="speG"
                     /locus_tag="ACHKUB_001879"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO6654905.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(6977..7687)
                     /gene="ynfC"
                     /locus_tag="ACHKUB_001880"
     CDS             complement(6977..7687)
                     /gene="ynfC"
                     /locus_tag="ACHKUB_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO6654906.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            7795..8100
                     /gene="ynfD"
                     /locus_tag="ACHKUB_001881"
     CDS             7795..8100
                     /gene="ynfD"
                     /locus_tag="ACHKUB_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO6654907.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            8299..10725
                     /gene="ynfE"
                     /locus_tag="ACHKUB_001882"
     CDS             8299..10725
                     /gene="ynfE"
                     /locus_tag="ACHKUB_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO6654908.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVIIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            10786..13209
                     /gene="ynfF"
                     /locus_tag="ACHKUB_001883"
     CDS             10786..13209
                     /gene="ynfF"
                     /locus_tag="ACHKUB_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO6654909.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            13220..13324
                     /locus_tag="ACHKUB_001884"
                     /pseudo
     CDS             13220..13324
                     /locus_tag="ACHKUB_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            13750..14382
                     /locus_tag="ACHKUB_001885"
     CDS             13750..14382
                     /locus_tag="ACHKUB_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO6654910.1"
                     /translation="MTSQLIPVFNGTIANETTLLVNARDLHTFLGVGKRFASWITERI
                     AEYGFVENQDYILVSPNREIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRNIQPVQTELQFTAEEIILLCYMQLWMEKAQDLSKHLYPIMKELNSSYTNK
                     LYDIAFETIYMVTKNRDALLREAAQLDQSSFVVQRARPMLKSLRARQFEF"
     gene            14430..14651
                     /locus_tag="ACHKUB_001886"
     CDS             14430..14651
                     /locus_tag="ACHKUB_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6654911.1"
                     /translation="MADIIDNAAEIEELQRNLSLQKYKSDSNAPSATHCCECGDPIDE
                     RRRQAVRGCRACASCQQDIELINKQRGVK"
     gene            14648..15355
                     /locus_tag="ACHKUB_001887"
     CDS             14648..15355
                     /locus_tag="ACHKUB_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6654912.1"
                     /translation="MNGVSYQALRLAAENATPGEWCTDDYGVIANAGLNANYYIASCS
                     GPDNRANKRFIAAANPATVLALLDERERNQQYIKSRDQENEDIALTVGKLRVELEAAK
                     SKLNEQREYYEGVIADGSKRIAELEKSEEQLINERDHAESALADMYFAATGDRPEWSN
                     CFSFSDAVDAVVDRIADLEAKQPSPVVPEGLVKAVRFYEQVKRENPPAETGAWKDAVD
                     WVLKEACQVVNIRIKGE"
     gene            15357..16112
                     /locus_tag="ACHKUB_001888"
     CDS             15357..16112
                     /locus_tag="ACHKUB_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654913.1"
                     /translation="MIHYHGGPITPDTCAMRAWKGRHAFISFAHSGQINLAAEYCQSF
                     ALDNGAFTAWKAAGKNKIDWSDYYEFVARWKNHPGFDFAIIPDVIDGGEEENDALLNE
                     WPHGKLAGVPVWHMNESDERFIHLCNEFPRVAIGSCGDYDVKRPTLAVARMKDLIRHI
                     VDEHGQPVTKLHGLRMLNPLIFTKLPLASADSTNVARNIGIDKAWSGAYAPASKETRS
                     ALMVERIEAHNSPGSLAYCEQRDRFEMQLQLAV"
     gene            16123..16752
                     /locus_tag="ACHKUB_001889"
                     /pseudo
     CDS             16123..16752
                     /locus_tag="ACHKUB_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206748.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            17043..17198
                     /locus_tag="ACHKUB_001890"
     CDS             17043..17198
                     /locus_tag="ACHKUB_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6654914.1"
                     /translation="MMQQKAMLIALIVICITVVVTALVTRKDLCEVGIRTGQTEVAVF
                     VDYESEK"
     gene            17318..17662
                     /locus_tag="ACHKUB_001891"
     CDS             17318..17662
                     /locus_tag="ACHKUB_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO6654915.1"
                     /translation="MKKVLIAALISGVSFGVFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGNERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(18095..26479)
                     /locus_tag="ACHKUB_001892"
     CDS             complement(18095..26479)
                     /locus_tag="ACHKUB_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO6654916.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VTTVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAEATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     AKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGR
                     QGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPEL
                     AQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDI
                     PAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAK
                     VWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFR
                     GVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYEPGEAAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDFMTEAQRARR
                     VFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRT
                     LKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKNRDSDNATIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGADASSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTANIGRTPPVL
                     RHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVM
                     LLDAVDKNGDPVVSAVHMKATDKRIEINKVASVYGTKGGMNKANSLDKAGLTLYRKEK
                     LSRDNPQYSGLQLPKEERSYRGSVDKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQ
                     MQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLL
                     ADYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQ
                     AQYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDGVVSLPR
                     TGKGFTIGGRESKQAMGRASRAQSPSTQAIQDLSESLIRSRKNEVGNAFLKLVQDNPD
                     KDYWQVFTDDRPDTMRVIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTYY
                     IKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAV
                     MNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKNLTGKGAQWQKVWKE
                     FVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVEN
                     ALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANL
                     VRTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYD
                     KVPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAA
                     GNVVGGILGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENIP
                     FGTPKPDSQLGIRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLIFWIDYISGGT
                     GRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAEL
                     KSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSSLSLAQQAAMVK
                     SVERDMKVAVDRFNREYNKKVGVE"
     gene            complement(26549..27814)
                     /locus_tag="ACHKUB_001893"
     CDS             complement(26549..27814)
                     /locus_tag="ACHKUB_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654917.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            complement(27825..28097)
                     /locus_tag="ACHKUB_001894"
     CDS             complement(27825..28097)
                     /locus_tag="ACHKUB_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO6654918.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGATIGATVGSAVPVVGTAVGAVAGAVIGGIAGALF"
     gene            complement(28107..28553)
                     /locus_tag="ACHKUB_001895"
     CDS             complement(28107..28553)
                     /locus_tag="ACHKUB_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654919.1"
                     /translation="MEYGKYETRARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNPQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(28556..29212)
                     /locus_tag="ACHKUB_001896"
     CDS             complement(28556..29212)
                     /locus_tag="ACHKUB_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654920.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELASNLTRSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(29307..29660)
                     /locus_tag="ACHKUB_001897"
     CDS             complement(29307..29660)
                     /locus_tag="ACHKUB_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6654921.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACFKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            complement(29796..31007)
                     /locus_tag="ACHKUB_001898"
     CDS             complement(29796..31007)
                     /locus_tag="ACHKUB_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6654922.1"
                     /translation="MKKASKLLLALIPALSLSVFSAGVYAADASGINGTSYYGVTVDQ
                     LKGHEYKNLIDFWKDLGFSGKAYLDGKVVNAGTKQPDSVNIQNTAGKYLKYSLFDYGE
                     SGLTNTELRAPRSNEIVSYYDYANETAYFVNTKTNQILFSADGEGVVTTVGADGKTYS
                     RHVPEISEYIYEIKMKSVNPLDSTIDTGLSAADVTDIHARLSSLGTGKTQVGGGATAG
                     GENATAVGYNAIANHTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAATYGQL
                     ERYRIAEENARIQGDAQTLKSANTYTDNRVSTLERSTNQQFRQLRDEVEKNRKRADAG
                     IAGAMAMTAIPVVEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASWDTQNG
                     SGVAAGMSVGW"
     gene            complement(31359..31499)
                     /locus_tag="ACHKUB_001899"
     CDS             complement(31359..31499)
                     /locus_tag="ACHKUB_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6654923.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(31731..32465)
                     /locus_tag="ACHKUB_001900"
     CDS             complement(31731..32465)
                     /locus_tag="ACHKUB_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6654924.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLSEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
BASE COUNT         8315 a         8563 c         7561 g         8057 t
ORIGIN      
        1 ttcctcttcg cagataagga cggcgaaaaa tacggtcgcg caaacaaact ttctggtctg
       61 gataaggcac tggctgctgg cgccactgaa atcacaaaag aagaatattt tgcccgaaaa
      121 aacggtacat actcaggttc acaacaaaat actggtgcat ctgacacgac cgcacaacca
      181 gagccgataa aagttaccgc tgacgaagta aacaaaatta tgcaggcagc caatatcagc
      241 cagcctgacg ccgataagtt gcttgctgca tcacgtggtg aatttgttga agggattggt
      301 gacccgaatg atccgaaatg ggttaagggg atccagaccc gcgattctgt gaaccagaac
      361 cagcatgaat cggaacggaa ctaccaaaaa gcggaacaaa acagcccaaa tgcgttacaa
      421 aacgagccag aaacgaaaca gcctgaatca gtggcgcaac aggaagtgga aaaagtctgc
      481 accgcctgcg gtcagaccgg cggcggcaac tgccctgatt gtggcgcggt aatgggcgac
      541 gcaacatacc aggaaacatt cgatgaagag tatcaggttg aagttcagga agatgatccg
      601 gaggaaatgg aaggcgctga acatccacac aaggagaaca ctggcggcaa tcagcatcac
      661 gatagcgata atgaaactgg cgagacggca gatcactcat ttaaggtgaa cggtcatcca
      721 gaaatcacat ccaccagcag gacgtgtgac catctaatga tcgaccttga aaccatggga
      781 aaaaatcctg atgccccgat catctcaata ggtgcaatat ttttcgatcc gcaaaccgga
      841 gatatgggac cggaatttag taagactatc gatctggaaa ctgctggcgg agtcattgat
      901 cggaacacca ttaaatggtg gcttaagcaa tcacgcgaag cgcaatctgc cattatgacc
      961 gatgaaatcc ccttagatga tgcactgtta caattgcggg aatttatcga cgaaaactcc
     1021 ggtgaatttt ttgttcaggt ctggggaaat ggagccaact tcgacaacac gattttgcgc
     1081 cgttcatacg aacggcaggg gatcccctgc ccgtggcgtt actacaacga tcgcgatgta
     1141 cgcacaatcg ttgagctggg gaaagccata gacttcgatg ccagaacggc tattccattc
     1201 gaaggtgagc gccataatgc acttgatgac gcccgttacc aggcaaaata cgtttcagtt
     1261 atctggcaaa aactgatccc gagtcaggct gattcttaat gttcaactgt cgccggttgt
     1321 gactggtatt ctgcaaccgg cgctcgtctg atgtaagaga taaagaaatc gatgagcgaa
     1381 gtaatcatga ttgtctctcc cggcaaatgg gtatccgaag agcagttaat tgcgctgaaa
     1441 ggaataaaaa aaggtacgtt aaaaaaggcc cgggaaaaat cgtttatgga aggaagggaa
     1501 tataagcatg tcgctcatga cggtatgcca tgggataaca gtccatgctt ttacaaccta
     1561 gaagaaattg atcgctggat tgagcgccag gcatcagcga gaccaagacg tcatcttact
     1621 tgactaaaag ccacactaac taatgagaga agttgaaatg aaatatccga caggcgtgga
     1681 aaaccatgga gggaaattac gtatctggtt tgtttataaa ggcgtaagag tcagggaaaa
     1741 tctgggggtt cctgacacag caaaaaacag gcgcgttgca ggtgaactac gcgcctctgt
     1801 ttgttacgca ataaaaactg gtgttttcga ctatgcaaaa cagtttccct cctcacgcaa
     1861 tctggaaaaa tttggtgagg cccgacaaga tttaaccata aaagaactgg ctgaaaaatt
     1921 tctggcactg aaagaaactg aagtcgccaa aacatcactc aacacatacc gtgccgtcat
     1981 caaaaatatc ctgagcataa tcggtgaaaa aaatcttgcc tcatcgatta ataaagaaaa
     2041 attactggag gttcgtaaag agctactgac tggataccag atccccaaaa gtaactatat
     2101 tgttacacaa ccagggagat cggctgtaac tgtaaataat tacatgacaa atcttaacgc
     2161 cgtgttccag tttggtgttg ataacggtta cctggcagat aatccgttta aggggatctc
     2221 gccattaaag gaatcaagaa gcattccgga tcctctttcg cgggaggaat ttatccgtct
     2281 tatcgatgcg tgcagaaatc agcaagcaaa aaatttatgg tgtgtttctg tttatactgg
     2341 cgttcgccct ggtgagctgt gtgcacttgg atgggaggac atagatctga aaaatggaac
     2401 aatgatgatc aggagaaatt tagcaaaaga ccgtttcacg gtaccaaaaa cacaggcagg
     2461 aaccaatcgg gtcattcatc ttattaagcc agcaatcgac gctctccgga gtcagatgac
     2521 attaacgaga ctgagcaaag agcatatcat tgatgttcac ctcagagagt atggcagaac
     2581 agaaaaacaa aaatgcacct ttgtttttca acctgaagtg tcagcgagag taaaaaatta
     2641 tggtgaccat tttaccgttg actcaataag gcagatgtgg gacgcagcga taaaacgtgc
     2701 cggactccgc catcgaaaat catatcagtc gagacatact tatgcctgct ggtcgctgac
     2761 agctggtgct aacccggcat ttatagcaaa ccagatgggc catgcagatg cgcaaatggt
     2821 atttcaggta tacggaaaat ggatgtctga aaacaataat gcacaggtag ctttgttaaa
     2881 tacacagtta agcgagtttg ccccaaccat gccccataac gaagcaatga aaaattaatt
     2941 taatatttat caaatagttg acacgtatga ctcttgaaat ccataaattc aagcgcagtg
     3001 cccagccatc ccgatactgc tgctttcacc aaatccttag tgcttctttc gtgtttttct
     3061 attgtcataa tggttatctc taaaaaagag gtaagatgcg tactacttac tcgccgttat
     3121 tggtattatt cagaaaaagt gagtaagact ttgcagcaat gtttttgatc ctgttcaaat
     3181 aaactaatgg catcagcaac atgctggaaa tcaaacgtat gggtaattaa tttttctggt
     3241 ttaattaacc ctttacttaa ccagtcgata acgaccggaa atttatttgc atttaagcgt
     3301 gaagagaaaa tagagagttc ttttccggta attccttgct gaatcacttc agacggttca
     3361 ctggagaacc ccatcaatac aatacgtgcc gctggagaag ccagcgttac ggcctctttc
     3421 aggatagaag gatgacaagc cgcatcgata attaatgtcg gcttgatgcc tttttcagtg
     3481 aaaatctcgc caagcggtgt ctggctgtta ttaatcgccc agtcagcccc gctctctttc
     3541 gctttttcca gtcgttcatc aatgcgatcg gcaacaatca catttttaac gttatagacg
     3601 ccttttaata cctgaacgat cgtcaggccg attggaccgg caccataaac cagaacggta
     3661 tcattttcag tcggttgacc atgaccggta acgttagccg caatggtaaa aggttcgatc
     3721 atcaccgcat attgatcggc cactgcttca ggaattttcc acgcattttt tgccggaacc
     3781 acggcatatt cactgaaacc accgtcagcg tgcacaccta aaacagccag tgtcgtacaa
     3841 acgttcggtt tacctataga gcacggatag caatgcccac agctgaccac cggatcgaca
     3901 gcaacacgtt caccgactct ggcgctttcc acgccgtcac ccactgcatc aatgacgcca
     3961 aagaattcat gaccaatgac gcgcggatat ttcgcaaaag gattatgccc acgataaata
     4021 tggctatctg aaccacaaat tccggcaagt ttcactttta ctcgtacttc acccgctgac
     4081 ggggtgggta tttcacgttc gacaatcgcc agttgattcg gtttttcaat taatatgctt
     4141 ttcattatct tactccttac cagttccaca gcgtgccatc ttccagacgt gcgactggca
     4201 gataagcagg ttcataggga tatttcgccg ccagcttttc atcgaattcg atgccaagac
     4261 ccggtttgtc tcccggatgc atatagccgt tatcgaaagt ccagttgtgc gggaagactt
     4321 cgagcatttg ttcggaataa cccatgtact cctggacacc gaaattgggg acccacagat
     4381 caaagtgcag cgccgcagcc atgcagactg gtgacaaatc ggaaggaccg tgtgagccag
     4441 tacgtacctg atacagcgaa gcaaaatcgg caatccggcg cataccggta attccgcctg
     4501 catgggtcag cgtggtgcgg atataatcga tgagttgctc ttcaatcagt tgtttgcagt
     4561 cccagatgct gttgaagact tcacccactg cgatgggtgt gacggtatgt tggcgaatga
     4621 gacggaagca ttcctggttt tccgcaggcg tcgggtcttc catccagaac atgcgataat
     4681 cttcaatgct tttaccaaag cgcgccgctt caataggcgt taagcgatgg tgcatgtcat
     4741 gcagcaaatg ttcattaaaa ccaaacttgt tacgtaccgc gtcaaacaat ttcggcatga
     4801 aatcgaggta tttctccgtc gaccacagct gctcttccgg ccactgtcct ttggttgcgg
     4861 gttcataagc cagaccttta cctttcgaca tgccgtaggt ggttttcata ccagggattc
     4921 cgcactgcac gcggatggct ttgaatccca gctcctgatg acgggcataa tcatccagag
     4981 cttcatcaat actgtgaccg gtggtatggc aataaaccat caccccttca cgagacgcgc
     5041 cgccgagtaa ctggtaaagc ggcatgttgg cagctttggc tttaatatcc cacagcgcca
     5101 tatcaaccgc tgaaatggcc gacatcgtaa ccggaccgcg acgccagtaa gcacctttat
     5161 agaaaaactg ccagatatct tcgatacggt gcgcatcgcg accaataagc tgcggacaaa
     5221 ggtgatcctg caaataagag gccacggaaa gctcacgtcc attgagggtg gcatccccaa
     5281 ggcccgtaat accgtcctca gtggtgattt ttaatgtgac gaaattacgc cccggacagg
     5341 taacaaaaac ttcagccttt acgatcttca tgttcgattc cttgcatcgc ttgtcgtgat
     5401 gcatgaaatc tacgcaactg agctactacc atacaagtat gaagatcgaa aaaagccgga
     5461 gtgatcacaa aaaaaggcgt atatttgcgc tgtgaatggt tgacaaaaga tgaaatagaa
     5521 taccttttgt cagctgacac ttcctcttat cttattgata aaatggattt atgttcctac
     5581 gtgcgccccc agcccgcaac aatgatcaac atgccgcaaa gcgcaatcaa cgcacccgtc
     5641 cagtcataaa gagtcagttt cacgccatcc acaacgcgca gccacatcaa cgccgtgcag
     5701 acataaacgc caccataagc cgcgtaaaca cgcccactcg ccgctggatg caacgttaac
     5761 aaccagacaa acagcgccag tgaaatcccc gccggaagca acagccagat actggcgttt
     5821 cgttttaacc acaaccaggg cagaaagcat ccaataattt cacacagcgc agtagcaaaa
     5881 aatagtaacg ttgttttaat catctttgtc tcttattgac atcatgtata gttatagggc
     5941 gacataatat catcaatata aacaccctcc tggtacgttt tgcgtccgca gtggatggtg
     6001 tagaatcacc tttaatcatt catacaggga atgaattatg aaaatcactc tcagcaaacg
     6061 aatcggcctg ctcgctattc tgctgtcttg cgcactggca ttgagcacaa ctgttcatgc
     6121 cgaaactaac aaactggtga ttgagtctgg cgacagtgca caaagccgcc agcacgccgc
     6181 tatggaaaaa gagcaatgga atgacacgcg caatctgcgc cagaaagtga ataaacgcac
     6241 tgaaaaagag tgggataaag ccgacgccgc ttttgataac cgcgataaat gtgagcaaag
     6301 cgccaacatc aatgcctact gggagcccaa tactttgcgc tgcctggacc gtcgaactgg
     6361 ccgcgttatt accccctaac ctgttattga tttaaggaat gtaaggacac gttatgccaa
     6421 gcgcccacag tgttaagcta cgcccgctgg agcgtgaaga tttacgctat gtacatcaac
     6481 tcgacaacaa cgccagtgtg atgcgttact ggtttgagga accctacgaa gcctttgttg
     6541 aactctctga tctgtatgat aagcatattc acgatcagag cgaacggcgc tttgtggtgg
     6601 aatgtgacgg cgaaaaagcc ggtctggtgg agctggtgga aattaaccat gttcatcgcc
     6661 gcgcagaatt tcagataatt atctccccgg agtatcaggg gaaaggtctg gcaacccgtg
     6721 ccgccaaatt agcaatggac tatggcttta ccgttctcaa tctctataag ctgtatctga
     6781 tcgttgataa agagaatgaa aaagcgattc acatttaccg caagcttggc ttttcggttg
     6841 aaggtgaatt gatgcacgag ttctttatta atggtcaata tcgtaatgcc attcgcatgt
     6901 gtatattcca gcatcagtat ctggcagagc acaaaacacc gggtcagact ctcctgaagc
     6961 cgaccgcaca atagcattaa taataatcga tcgtattttt gatggtgtaa acccgttcga
     7021 cggcgggttt tactccttca tcaacaatga ttagctgaca gtccaccgga ttagcgtgac
     7081 tgtcatattc acagctctgt tttacattac caacccgttg attattcagt aaagtaaccg
     7141 ctgtgtaatc taattttttg atcggatccg ttgatggcgt ggcgctgaca gataatgttt
     7201 tgtcgttact tttcgtggtt ttacccagcg gataaccctg atcatcatag cgatattcca
     7261 tctgcatttg tttgctgctg gctttaatca cgaagccatt atcatcggtt tcccaactca
     7321 ccccggcaga aggtaattct gctagctggc attttccctg taaacgtact ctcttctcca
     7381 gcgtctcggc atcacggtaa taattggcgt ccagtaccag agcgaccacg gtattatttt
     7441 ccagatccag taattcgagt gaatcaaaac agccttcttc cgacaaagtc ccagaaacac
     7501 gtttcgtcac ttcaccttgc tcatccatta atgtctgagt gaaatctttt accggaccac
     7561 gcagcggatc aaaatcgaat tcattagaga aactggccat ctcaggggta aatgaaagtg
     7621 ttacctctgt gcggtcacat cctgtgagga atatcgcgag taagcatggt agtaatttgt
     7681 atttcacaac agtcaccaga gagtagagat gattctcaat catagtagca aatacagtac
     7741 tttacacgtt aaatgctatg cttaaagaag ttatcttcgc gtaaggagct tatgatgaaa
     7801 ctctcaacct gctgtgccgc gcttctgctc gccctcgcct cacccgcggt actcgccgcg
     7861 ccgggatcct gtgagcgcat acagagcgat atatcacagc gcattatcaa taatggcgta
     7921 cccgaaagca gcttcacgtt aagtattgta cccaatgacc aggttgatca gcctgattcc
     7981 caggtcgtcg ggcattgtgc taatgatacg cataaaattc tctacacccg caccaccagc
     8041 ggtaacgtct ctgctcccgc gcagtctacc caggatggcg cgcctgccga accgcagtaa
     8101 tacattatcg actgaacgcc ggatacgaca catccggcga tttgaacata caacataatc
     8161 ccaccttatt actcataccc ttctattgat atggattaat aattcttaac ccaaaatggg
     8221 tagactccct ctattgttag cgcgctaaat attcaatata taaactttta tataacgata
     8281 aagaacaggg agtgagttat gtccaaaaat gaacgaatgg tgggcatcag ccgcagaaca
     8341 ctcgttaaat ctaccgcgat aggttctctg gcgctggctg caggcggttt ttctttgccg
     8401 tttaccctgc gcagtgcagc agcagcggta caacaggccc gcgaaaaagt ggtctggggt
     8461 gcctgttccg tcaactgtgg tagccgctgt gcacttcgtc tacatgttaa agataatgaa
     8521 gtgacctggg tggaaactga caataccggc agcgatgagt acggcaacca tcaggtacgc
     8581 gcctgtttgc gcggtcgctc catccgccgg cgtattaatc atcccgatcg cttgaattac
     8641 ccaatgaaac gcgtgggtaa acgcggtgaa ggcaaattcg aacggattag ctgggatgaa
     8701 gccctggata ctatcgccag tagcctgaaa aaaaccgtcg aacaatatgg caatgaggct
     8761 gtatatattc agtactcttc ggggatcgtt ggcggcaata tgacccgctc ttcgccatca
     8821 gcctcggcgg tcaaacgcct gatgaactgc tacggcggtt cactcaatca gtatggctcc
     8881 tacagcaccg cgcaaatttc ctgtgccatg ccctacacct acggcagtaa tgatggcaac
     8941 agcaccacgg atattgaaaa cagcaagctg gtcgtgatgt ttggtaacaa cccggcagaa
     9001 acccgcatga gcggcggtgg catcacttat cttcttgaaa aagcgcgcga gaaatcgaac
     9061 gccaaaatga ttgttatcga tccgcgatat accgatacgg ctgctggtcg tgaagatgaa
     9121 tggctcccta ttcgcccggg caccgatgcc gcgctggttg cgggtattgc ctgggtattg
     9181 attaacgaaa atctcgttga tcaacctttt ctcgataaat actgcgtcgg ctatgacgaa
     9241 aaaaccttac ccgcagatgc acccaaaaat ggtcactata aagcctatat tcttggtgaa
     9301 ggtgatgata acacagcgaa aacgccgcag tgggcttcgc aaattaccgg tatcccggta
     9361 gaccgtatca tcaaactggc gcgtgaaatt ggcacagcaa aacccgctta tatctgccag
     9421 ggctgggggc cgcaacgcca ggcaaacggc gaactgactg cacgcgctat tgctatgtta
     9481 cctattttga cgggcaatgt cggcatcagc ggcggaaata gtggcgcgcg tgaatcgacc
     9541 tataccatta ccatagaacg cctgccggtg ctggataatc cggtcaaaac gtcaatctcc
     9601 tgcttcagct ggacggatgc tatcgatcat ggtccgcaaa tgacggcaat ccgcgacggt
     9661 gtccggggca aagataaact ggatgtgccc attaagttca tctggaacta cgcgggaaat
     9721 accctcgtta atcagcattc tgacatcaac aaaacgcatg aaattctgca ggacgaatcg
     9781 aaatgcgaaa tgattgtgat catcgaaaac tttatgacct catcggcaaa atatgccgac
     9841 attctgctgc cagacctgat gaccgttgag caggaagata ttattcctaa cgactacgcc
     9901 ggtaacatgg gatatctcat tttcctccag cctgtcacca gcgaaaaatt cgaacgcaaa
     9961 ccgatttact ggatcctgag tgaagtcgcg aaacgtcttg gacctgacgt ctatcaaaag
    10021 ttcacagaag gtcgcacgca ggaacaatgg ttacaacatc tgtacgccaa aatgcttgcc
    10081 aaagatccgg cgttaccgtc ttacgacgaa ctgaaaaaaa tgggtatcta taagcgtaaa
    10141 gatcccaatg gccattttgt cgcctacaaa gcatttcgtg acgaccccga ggcaaatcca
    10201 cttaaaacgc cttccggtaa gattgaaatt tattccagca ggctggcgga aattgcccgt
    10261 acctgggaac tggaaaaaga tgaagtgata agcccattgc cagtttatgc ctcaaccttt
    10321 gaaggctgga actcccctga acgtagaacc ttcccactgc aactgtttgg tttccattac
    10381 aaatcccgta ctcactcgac ctacggcaat attgatctcc tgaaggctgc ctgccgtcag
    10441 gaggtgtgga tcaaccctat agatgcgcag aaacgtggga ttgccaacgg cgatatggtg
    10501 cgggtgttta accatcgtgg cgaagttcgg ctaccagcca aagtaacacc acgtattctc
    10561 cctggagtta gcgctatggg ccagggagcc tggcacgagg ccaatatgtc tggcgataaa
    10621 atcgaccatg gcggctgtgt gaatacgcta accactctgc gcccttcacc actggcgaag
    10681 ggaaacccgc agcacactaa tctggtcgag atcgaaaaaa tataacccac gacaaccata
    10741 aattctggca tgacattttg tttgaaaagc aataagtgag taatgatgaa aatccatacc
    10801 acagaggcgc tcatgaaggc tgaaatcagc cgtagaagtc tgatgaaaac ctccgcactt
    10861 ggcagtcttg cgctggcaag cagtgctttc actctgccat tttcccaaat ggtccgggcg
    10921 gcagaggctc cggtagaaga gaaagcggtc tggagttcct gcaccgttaa ctgcgggagc
    10981 cgctgtctgt tacgtttgca tgtgaaagat gacaccgtgt actgggtgga gtctgatacg
    11041 acaggtgacg acgtctacgg taaccatcag gttcgagcgt gtttacgcgg gcgctctatc
    11101 cgccgacgga tgaatcatcc tgataggttg aaatatccca tgaagcgcgt cggcaagcgc
    11161 ggtgaaggta aatttgaacg gataagttgg gacgaagccc tggataccat cagtgataat
    11221 cttcggcgga tcctgaaaga ttacggcaat gaggctgtac atgtcctgta cggaacaggc
    11281 gtagatggcg gaaacatcac caactcaaac gtcccgtacc gtctgatgaa ctcttgcggt
    11341 ggttttctca gtcgctatgg cagctacagt accgcacaga tcagtgccgc aatgagttat
    11401 atgttcggtg ccaatgatgg caacagcccg gatgatatcg ccaatacgaa actggtcgtt
    11461 atgttcggaa ataacccggc agaaacgcgg atgagcggcg gtggtgtcac ttactacgtc
    11521 gagcaagccc gcgaacgttc aaacgcacgc atgatcgtca tcgatccacg ttataacgac
    11581 actgctgccg ggcgtgaaga tgaatggctg cccattcgcc ctggcaccga tggcgcactg
    11641 gcctgtgcga ttgcctgggt actgattact gaaaacatgg tcgatcagcc atttctcgac
    11701 aaatattgtg ttggttacga tgaaaaaacg ctgcccgcca acgcaccacg taacgcgcat
    11761 tataaagcct atattctggg cgaagggcct gacggcatag ctaaaacgcc ggaatgggca
    11821 gcaaaaatca ccagcatccc ggcagaaaaa attatccagt tggcacgaga gatcggttca
    11881 gcaaaacctg cttatatttg tcagggttgg gggccacaac gacattccaa cggcgagcaa
    11941 acgtcccgcg ctattgccat gctttccgtt ctcaccggta acgtcggcat aaacggcggc
    12001 aactcaggtg tacgcgaagg tagctgggat ctgggggtag aatggttccc gatgcttgag
    12061 aatcctgtta aaacgcagat ttccgtcttt acatggacag atgccatcga ccatggtacg
    12121 gaaatgaccg cgacccgtga tggtgttcgt ggaaaagaaa aactggatgt ccccatcaag
    12181 tttttatggt gctacgccag taacacattg atcaatcaac atggcgacat caatcacacc
    12241 catgaggtgc ttcaggatga cagcaagtgc gagatgattg ttggcattga ccacttcatg
    12301 acggcctcgg ctaagtattg cgatatcctg ttgcccgacc tgatgccaac agagcaagaa
    12361 gaccttatct ctcatgaatc tgcagggaat atgggctatg tgatcctcgc ccaacccgca
    12421 acctcagcaa aatttgaacg caaacccatc tactggatgc tgagtgaagt cgccaaacgc
    12481 ttaggaccag acgtttatca aacctttact gaaggtcgca gtcagcatga atggatcaaa
    12541 tatctccatg cgaaaacgaa ggaacgtaac cctgagatgc ccgactacga ggagatgaaa
    12601 acgaccggga tctttaagaa aaaatgcccg gaagaacact acgtcgcttt ccgcgcattc
    12661 cgtgaagatc cacaggcaaa cccgttgaaa acaccttcgg ggaaaatcga aatttattcg
    12721 gaacgactgg cgaagattgc agatacctgg gaattgaaaa aagatgaaat tattcatccc
    12781 cttcctgcgt ataccccagg ttttgatggc tgggacgatc ccctgcggaa aacctatcca
    12841 ctgcagttaa cgggcttcca ctacaaagcg cgtacccact ccagctacgg caatattgat
    12901 gtgttacagc aggcctgccc acaagaggtg tggatcaacc ccattgatgc tcaggcacgc
    12961 ggtatccgtc atggcgatac cgtgcgggta tttaacaata atggagaaat gctgattgcc
    13021 gcaaaagtga ctccgcgtat tctgcctggc gttaccgcca tcgggcaagg tgcgtggctt
    13081 aaagcggata tgtttggtga ccgggtcgat cacggcggca gtatcaatat tctgacctct
    13141 caccgccctt caccgctggc aaagggaaac ccgtcgcaca gcaatcttgt ccagatcgaa
    13201 aaggtttaag gagtagccca tgaccacaca atatggattt tttatcgatt ccagccgctg
    13261 tactggctgt aaaacctgcg aactggcgtg caaagatttt aaagttggtt acgtatcgtt
    13321 taaaatcaat tagatagccc gtcatttctg cgctcacacg tcccaacatt gaaaaacatg
    13381 caaagctttg taagccgatg caaagctttg tgtgtctcac ttttgtccca ataccaatcc
    13441 gaatccaccc tttgtcatca ttgagaaatg gcaaggaaga aaaggatgtt actgcacgat
    13501 ttgacaaatc cgccagagct atcgcatact gaccgcactg aacttacaag cggtcatccg
    13561 cacccgatag ctttgcggct tttttatgcc cgtaatgcgg catagtcaca tccgtacaaa
    13621 ggtcgggtgg agaggcgtaa tacaataccc gtaaggggaa tatgcccaga gcttcttgta
    13681 aggctcagtt gacacccgac caaccagcta ctaactggtt gagctaacta aacttacaag
    13741 gaggtcatca tgactagtca actcatcccc gtattcaacg gcactatcgc caacgaaacc
    13801 actcttctcg ttaatgcccg tgatttacat acttttcttg gggtaggtaa acgctttgcg
    13861 tcttggatta cagaacgcat tgctgaatat ggtttcgttg aaaatcagga ctatattttg
    13921 gtttccccaa atcgggaaat caaaggtcga ggaggcgatc gccgtagcaa agactatcac
    13981 ctcaccctcg acacagccaa agaaacggcg atggttgagc gtaacgaaaa aggccgccag
    14041 atccgccgct acttcatcga gtgcgaaaag aaacttcgca acattcagcc agtacaaact
    14101 gagctgcaat tcacagccga ggaaatcatc ctcctctgct acatgcaact ctggatggaa
    14161 aaagcccagg acctcagcaa acacctgtat ccgatcatga aagagctgaa ctcatcatac
    14221 acgaacaagc tgtatgacat tgcgtttgag actatctaca tggtgacgaa gaacagagat
    14281 gcgttactga gggaggctgc acagcttgac cagtcaagtt tcgttgtcca gcgggccaga
    14341 ccaatgctga aaagcctgcg ggcaaggcaa ttcgaattct aaaaccaaag gagcttcggc
    14401 tccttttttc atgcctgaag gaaaggaaaa tggcagatat catcgacaat gccgctgaaa
    14461 tcgaagaatt gcagcgcaat ctctccctgc aaaaatacaa atccgatagt aatgccccat
    14521 ctgctactca ttgttgcgag tgtggcgatc cgatagatga gcggcgacgc caggctgttc
    14581 gtggatgccg agcttgcgca agttgccagc aggatattga acttatcaac aaacagagag
    14641 gtgtgaagtg aacggagtta gctatcaagc actgcggctg gcggcagaaa atgcaacacc
    14701 aggcgaatgg tgtactgatg actatggggt tattgccaat gccggactga acgccaatta
    14761 ctacatagca tcatgctcag gaccagataa tcgcgccaat aagcgattca tagcagcagc
    14821 caatccggct accgtcttgg cactgctgga tgaacgggaa agaaaccagc aatacatcaa
    14881 atcacgcgac caggagaacg aggatattgc gctaacggta gggaagctgc gcgttgagct
    14941 ggaagcagca aaatcaaaac tcaacgagca gcgtgaatat tacgagggtg taatcgcgga
    15001 tggaagtaag cgcattgcag aactggagaa aagcgaagag caactcatta atgagcgtga
    15061 ccatgctgag tctgctttag ctgatatgta ctttgcagca accggggata ggcctgagtg
    15121 gagtaactgt ttcagttttt cagatgctgt cgatgccgtg gttgacagaa ttgctgattt
    15181 agaagctaaa cagccatcgc cagtagtgcc ggaaggactg gttaaagcag tgcgcttcta
    15241 tgagcaggta aaacgtgaga atccgccagc cgaaacaggt gcatggaaag atgctgttga
    15301 ctgggtactc aaagaggctt gtcaggttgt aaacattcgc atcaaaggag agtgatatga
    15361 ttcactacca cggtgggcct attactcctg atacgtgcgc aatgagagca tggaaagggc
    15421 gacatgcgtt tatcagtttt gcgcattcag gccagatcaa tctcgctgct gaatactgtc
    15481 agtcgttcgc gctggacaac ggtgcattca ccgcctggaa agcagctggc aaaaacaaaa
    15541 tcgactggag cgattactac gagtttgttg ctcgctggaa gaatcaccca ggattcgatt
    15601 ttgccattat cccggatgtt attgatggcg gagaggagga aaatgatgcg cttctgaatg
    15661 agtggcctca cggaaaacta gctggcgttc cagtgtggca catgaatgaa agtgacgagc
    15721 gatttattca tttgtgcaat gagtttccgc gagtggctat cggtagttgt ggcgactatg
    15781 acgtaaagcg cccaactctt gcggtagcca gaatgaaaga cctgattcgt cacattgttg
    15841 atgagcatgg tcagccggtt acgaaactac atggattgcg catgttaaat ccgctgatat
    15901 tcacaaaatt acccttagcc agcgcagata gtacgaacgt cgctcgaaac atcggtattg
    15961 ataaagcctg gtctggggct tatgcacctg caagtaaaga gacacgctca gcattaatgg
    16021 tagaacggat tgaggcacac aatagccctg gttctcttgc gtattgtgaa caacgcgacc
    16081 gctttgaaat gcaattgcaa ctggcagttt aaggactaac aaatgaccac tataaccaaa
    16141 gagcgactgc tgacaatcaa gcagtggcgc gaaacatacg gacctggtag caacgttgta
    16201 ctgccagcag aagaagcgga agaactggca cggattgcac tggcatcgct ggaagcagag
    16261 cctgtagcgt aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt cggtgagtat
    16321 gtgggtcact cgctacctga tggaacgcac cagctttaca ctgcccctcc agcgccagta
    16381 gtacctgaag aaaaaccaat gcctaaccct cttaaaatgt acgcggtcga tgctgttgcc
    16441 gctattgcag aggtgagagg ctggaactcc tgccgcgcct ccatgcttca tggtgccgaa
    16501 cctgtaagcc aaacttacaa gttgaacgag ctgtcgagca actctccggt aactccggct
    16561 cgttggataa gctgtagtac acgaatcccc gctcaagatg attggatttt aatttattca
    16621 aagcacggcg agtatatggc aggacaggta caaggggaat acgtggagtt gagcgacggc
    16681 actttatcgt ggttagggag cgccttattc tggatgccgc tgccagaacc gccgaaggag
    16741 gtacgccaat gagctagcct gaagccttct caaatgttgg tattgcaatg gcggtagcgc
    16801 tgatggtgta ttcgatttgc cgctggggat aaaaacggtt tgcggggaaa gaagagttaa
    16861 gtagaattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccgccgt aaggcagaca
    16921 tagaaaagcc ccagttaaca tttcgcgtct tgcaggacgc ttaacattaa tctgaggcca
    16981 atttcatact agacacatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagt
    17041 ctatgatgca gcaaaaggcg atgttaatcg ccctgatcgt catctgtatt accgttgttg
    17101 tgacggcact ggtaacgagg aaagacctct gcgaggtagg aatccgaacc ggtcagacgg
    17161 aggtcgctgt cttcgtagac tacgaatctg agaagtaaga gacctggcga gggagtaatc
    17221 tcccgccacc tctgatgtgt caggcatcct caacgcaccc gcgctttacc atactgaaaa
    17281 tgctgtttga atgttcatct ctgaaagagg actatgaatg aaaaaggtat tgattgcagc
    17341 acttatttcc ggtgtgtctt ttggcgtttt tgcacagcag ggcggtttcc aagggcctga
    17401 agcagagcgt tcaacagtag cgcaggcaaa agaactgaag gatgatgcat gggttatcct
    17461 tgaagggagc atcgttaaaa aagtgggtaa tgaacgttat gagtttcgtg acaatagcgg
    17521 gacaattgtc acggatattg atgacagcgt atgggccggg cagaatgttt ctccgaaaga
    17581 caaagtaaga attgagggtg aaattgataa agacctgagc agtgttgaag tggatgtaaa
    17641 ggcactgaaa ttattaaagt aaccgtccca gcttgctaag cccgtcttac tgacgggttt
    17701 tctgtttgtt atcacagtat tttcaacaaa acaccgaatc ttatccggta cgctgtaaaa
    17761 accccgtcct tcagggcgtg gaggatgtca aatggcaggg gtgaacatca tcccggttac
    17821 atcagatcgc ctaataacat atcaagatta ttttgctcat aaatgctatt cgccattacc
    17881 gttgctgctt cagcttaatg aaacgcatag atccgccatg ctttttcagc aaatacgctg
    17941 ccatttgcgc aactttttct tcattaaaca taaatcggct ctctgtatca catgaaatgc
    18001 tctttctgac tatatgttaa tagctccagt tattacaggt caatgagtgt ttatttctta
    18061 atcagccccg cacttccgta cggggcatgt tctgttactc cactcccact tttttgttgt
    18121 actcgcggtt aaagcgatcc acggcaacct tcatatcccg ctctaccgat ttcaccatcg
    18181 ccgcctgctg cgccagactg agagaactgt cggcataaat ggcatcgcgc tgtttgcgca
    18241 aatccttcag tctcttccgg gtatcctgca taagcccgtt catcgacaat tttccgttgt
    18301 tctcgtcaat gaacgccgtt ctttctgcac cggtcagact cttcagctct gcgtgatact
    18361 gcgcaacctc tgtcatccgg tcgtacatct tctgctggtc tgcatacggc atcacctcac
    18421 ccgaaatttt ccccaggaag ggcacctgct gttccggtat atcaatacca ttcagcgatt
    18481 tcaccgccgc atccgtggtt ttggaaatga agcgccctgt ccctccggag atatagtcaa
    18541 tccagaatat cagcgattcc ggtgtgatat ccaccgcgcc gggacggtac tgactgccac
    18601 cagagaacgc attcagccaa gatgcaaacg ccttgtacgc ttctggcgtt gaacgtattc
    18661 ccagctggct gtcaggtttt ggtgtaccaa acggtatgtt ctcctggtaa atctgcgccc
    18721 ccatgaagtt ttcattcatg gcaaggttcg caaacggacg cagaatggtc ggcgctgcat
    18781 ttttcagcaa tgccccggac agtgtttccg acgtctcact gccgatcggg ctgaatgccc
    18841 ccaggatacc accgacaaca ttaccggcag cacgggatgc cgtcaggtca cccgccgcca
    18901 caccttcagc ggtatgcccg agcaggaaga aaacgttgta cccgtaaggc agaggaatac
    18961 tccagtactc tccggccttg cccccgaaca ccgatttcat aatgacgagg ttacgctctt
    19021 tcacatgaga cggcactttg tcataccagt taaccccgtc atcatcctcc cccgcaacac
    19081 tgcggttaag cgagccaagc agataacccg ctcccacagc tgcaagcgcg attttctgcg
    19141 gtacattgag attcttccag cgaaggcgct ccagtaacgg cccgtcgcca ttaagatgtc
    19201 cgagcgttct caccaggttt gccgtcccct gaatgctggc gttggcaaac atatacagcg
    19261 agttcatcag cgctccctgc tcaccacgac gattaaagtt caccgtcatg tttttggcaa
    19321 gagacgccgc ctgctggcgt gacaaaccgg catcacgggc gtgtttatag gcagaaagac
    19381 gcagagcatt ttcaaccgcc ccgttggcat cctcgacaag gttaaggaac gaattccatg
    19441 caccgatact ctggcctttc catcctccct tcgccagcga tacaaggcga tccatttcct
    19501 tctgctggcc ttcaaggtca cccatgttaa accagccggt tttacctccg tcctcaacaa
    19561 actctttcca caccttctgc cactgcgcac ctttgcccgt gaggtttttg ccacgcagac
    19621 tggcgtatac ggctgacata gcagaacggc tgtctttcac cacagccagt gcggataagt
    19681 tatccagccc tttcagtttg ccgtcgctcc ttccctgctc cgccttcaga ttcatcaccg
    19741 ctgtctgcac atcacggatg aagttactga ccaggaattc cgggttatac gacgtgttca
    19801 ccgttgccag gaagcggtta actttcccca gcgtacggat tactgcgtta ctggtttccg
    19861 gtcccatatt cttcatcgca cgcatcaggc gcggatcatg gagtttgatg tagtacgttt
    19921 tgccgttctt tttggtggtg aagtaccggt ctgccatcat tgccatcgcc accggacgct
    19981 cgacaacttc gcgaatggtt tcaccagttt cctggtcctt gcgctctgca atcacacgca
    20041 tggtatccgg tctgtcatca gtgaatacct gccagtaatc cttatcggga ttatcctgca
    20101 ccagcttcag gaaggcgtta cccacttcat ttttgcgact gcggatcagc gattcgctca
    20161 agtcctgtat cgcctgagtg gacggagact gtgcgcggga tgcacgcccc atggcctgct
    20221 tactttcacg cccaccgatg gtgaagccct tacctgtacg gggcagtgac accacaccgt
    20281 caacatcctg ccctttcaag ggaacgtagt aacggtaggc gttctgccag gcatccacca
    20341 caccgctctc ttccagtccg gcctcacgga taagctcacg gcgacgggcc agcatatcgt
    20401 caacaatccc tgccagacgg tcatactgtg cctgtttgcc gctgttacgt acacgctgca
    20461 tgatttccgc cgcttccgcg ttggtcatcc ccgaaccgcc gtccggcatt ttcgggttga
    20521 ttttcgcgat atgcgcgtta cgttccggcg cgtgacgggc gtagagatac tcatccagat
    20581 cggcctgcgc aattttgtag tccgccagta atttagccag tggctgaacg tagcgctcct
    20641 tcatcacgtt caggtcgttt tccgccttcc cgtggaagag ttcttccgcc atataagcgt
    20701 tgttactgtc gtctattttt ccgccagttt tacggatatt ctcctgaaca gctttcagca
    20761 cctggaattt atcctgcatc tggcgcacaa aacgcgatgc aattgtctct tccggtgtca
    20821 gactgctggt acgggagtaa tacggcccct tgcgaatatc ttcaggatag agtattttat
    20881 ctacagagcc gcgataagaa cgctcctctt tgggcaattg aagcccactg tactgagggt
    20941 tatcgcggct taatttttcc ttccggtaaa gcgtcaaacc tgctttatcc aggctattag
    21001 ctttgttcat ccctcctttt gttccatata cagaagccac cttgttaatt tctattcgtt
    21061 tatctgttgc cttcatgtgc actgccgaca ccaccggatc accattttta tccacggcat
    21121 caagcagcat cacaaccgca tttctttctg tcgcggaacg gtaaattgca tccggatcgt
    21181 gcatcagctc aggaagtctc tcgataacat ccatcggcac cacatgtttc acgccattgg
    21241 ttgccttccg cacagtgtcg cgggagataa ccagcggcaa atctggtgcg ccaaggtggc
    21301 gcaataccgg cggcgtacgc ccgatgtttg cagtcattgc accagaacgt aacgacttca
    21361 tcattctggc aaggtcatca cgataacgct cgccctcacc ttccggcact ttgaacggat
    21421 caggtttacc actacgggag tactgagacg atgcgtccgc gccatcctca cgcggcgtgt
    21481 aaccttcccg cacacgctgg cctaacgtac ggatggtctc gcgaacaagt ctgatatcgt
    21541 tcagttccgt cggcttcagt aaccccgtac gacgcagtac ccctttgacc agggcaacaa
    21601 cacgctccca tgccgccacg aatttattcg gctgtttctc cgccatatgt gccagaaatt
    21661 cacccgcctg cacttccggt gattccttac cataagacgc atcaacctta cgccaggctt
    21721 cacggatggt ggcgttatca ctgtcacggt ttttcagcac ggtcttaata atcgtctgat
    21781 attccgctgg cgtgacaaca tgctccatgg catggtggat aatctcgtga cgcaacttct
    21841 cgcgtacggt ccgcccgtca gggatgttat ccgccaccag gacaatttct cgtttatccg
    21901 gacgatagaa tgcgtgcacc ctgccgtaac cgtcgaacga ttcacccgcc agcgcttcag
    21961 cctctttctg tgacttcacc acacggacct tcaggtcact gtccttaatg ccgctcatca
    22021 cgccacgggc aaccgcttca acctgcggga ccgggctgcc tttggcttcc gcactacggt
    22081 taacatccga aatgagatta ccttcaggtg tgcgggtaac gcccttacgg gaataaaacg
    22141 caacgccctt gtccgtctca cgggttttca gggtgcggaa caggtgatcg aatgcctcac
    22201 gaataccgcc atccagttcc gcattcgtcg gataagcgta ggtgttatct gtgttgtgct
    22261 caggtgcctt acggatattg accagataat cattctccac gccagccata cgcgccttat
    22321 cctgaacata acgctcaaag gcacgtgccg ccatttcaac atccgttgac cagtacggtt
    22381 ttgagcgcac ctcatcaaga agcgctgaac gacgcggcat gtcactgttt ttaatggcct
    22441 gaatcacacc tttaaaagcg tcgtaaacct cctgacgtac cggatattca gcatcaacat
    22501 acctgccgtc tttaaatacg cgcctggcac gctgtgcttc cgtcataaag tcgccacctg
    22561 acgtaatttt cccgtcagtg gaaacgtcat aacgaccaaa ataattatcc agagaatgga
    22621 accattcgtg cgccagcgca cccggtccgt taccttttgt caggttgatt gccgcctcac
    22681 ccggctcata gtgtgctgcc gccttaccct taccacgggc accaaatgcc aggccaagac
    22741 gaccgttcag ggaaagcgct tttgtcggta cattcagtac ttccgcaagg tcatgcagcg
    22801 agtcataagc ccggttcaaa tcagcctgac gacgcggacc ttccacataa ttaccaaact
    22861 gcacaccacg gaaaccaaac gcatcactga actgctccgg tgaaacattc cccttgcggc
    22921 gttctggtcc ggtacggtcg cggttggtgg cgttgcgctg ctcctcacgc gaaatctccc
    22981 gcatctcctt cacatgacga acaagctcat cacgatgtga atcaatgtac ttacgcgcat
    23041 cactggctga cttaaagcca cctctcaccc gcattttgtt tttaccgtaa gcgataaaaa
    23101 tatcgccact gcgggtattc cggtaaacgt caaagcggat tttgtcatcc ggtgacggtg
    23161 ctgttttttc atcacctttc gcctgtgatt tttcctcctg ctctgcaaac cagactttcg
    23221 cctttgccag taattcatcc ctgctttccg agaaaaagag gttactcccc ttattgtcct
    23281 tattgcgcag tgaataaagt ttctgtggcg gatcgtaacg cttccctcct gccgcctgat
    23341 acacacccga taccacccga taagcagaag ctctgtccat ctgtgagggt ggcagagtgc
    23401 gtaacagttg ccaggtgtcc gcgtaacggg agggcattct gccttccatc cattctgcga
    23461 ggcgtttcgc gctgaccgtt ccgttcagca tttccgatac actgtgtcgt acttttttta
    23521 tgctctcccc ccagcctgcc gtattgtgtt tcgtcttcgc cggaatatcg ctacgataca
    23581 gcgctatcat tgccagggta tcagcatcgg caccttcgtt cgccagtttt gcgtagtccg
    23641 gtttcgggaa cagtttgctc agcggctgcg tggcataatc cctgtcttcc agcgttttac
    23701 ccagtgtttc agcaagctgt gcataacggt gtttggctgc ccctttaatt tcctcgccaa
    23761 agtcttcaat ttttttgccc ctgacttcac ctccgcgcac ggtagcttcc ctgtcagtag
    23821 agacagttgt ttcagattca ggtgcgattg tctcaggctg cgggatgtca cgatttcctc
    23881 cggcctgtcg gcgtacttct gcctgctgtg caagctcagg cagtgagtcc cgtacagtgc
    23941 ggggaagtga ttcaggctga ggcatacgaa cattacgcgc aatgtcagga gccggtagcc
    24001 cttcacgtac cggtgctgtc gcgttctgcg catccggtga cggcagacca cgacgaacca
    24061 tttcaccctc gagtgtttcc ccctggcgac cagcggcgtt ttccggtgcc ggagcttgtc
    24121 ctttctggaa actctggccc ttaacctcac cggttgtggt aaaacgacca ccacgtcctg
    24181 cctgattctc atccggcgta cgcgccacct ctcccggtaa cggatatccc tgtccgggat
    24241 gaatatcgcc gggagcgggc aggcgtggac gctcagttaa ctcctgtgct gtcggaccgg
    24301 catcaccttc agccatctgt gaacgtacaa gctcctctgc cgtcggcacg ttgccacggg
    24361 caaggcgggc ctgtacctcc gtatcatccc ccgtgaaacc acggaatcgt gggtcacgca
    24421 tgaacgcggg ctgctccatc gggtcagcat cagcaatacg ctctccgtca gccagggtgt
    24481 taatggtttc cgttgccaca tcttcagaga acgcaggatt atccatttca acggcatact
    24541 gattctcgcc tttcttcact acggacggtt tcaggccggt agcggcggca ttacggaaaa
    24601 catcactccc catggcgctt ttctcatccg tgaaataacg gttgtccggg cgcacctttt
    24661 caatgcgttc cgctggtctg gacgcaccat tcccgttaat ttcaatgcgg acttcattcg
    24721 ggtcccggaa ccgcaccgtc gggtaaatgc tcccgttacc atcctccgca ttttcaggct
    24781 gtgactgtgc ttccggcgtc acttcctgtt gtgggcgaat gacttctcgt attgcctgaa
    24841 actgttgttc ttcagtctcg gtgcgcccct ctttctggct gagtctgcgg tattcctcaa
    24901 gcagttcaga acgcggcttc gccttcagct cattcatcac agcctgtcgc ttcgcctgtt
    24961 cgtccagttc attcaacaac tggctggcag cttcccggcg atgagctgcg gatgcgtcac
    25021 cctctgttgc catatccgca tcagcatact gctccagaag ctgctcgcga ttcatgccct
    25081 gcatggattc acgctgctgc gccaccggat caacagattc tggttgcggg gctgcgtcgt
    25141 cctgctgcac ggtttcagca tcacgcatgg cggtttcttc tgcggcctga cgtctgccac
    25201 gatatccggc aaccgcaccg gatggagccc ccatcgcagc accaaaggct gcaccttcga
    25261 tcgttgcgtc agccacgccc tcccacggtg acacatccat tccggcggtc tcacgcaatg
    25321 ccgtgttttc ctgatagcgt gaatagccgc cctgcgccgc attaatcgcc ccctgttccg
    25381 tggcatttct gacaatgccg cttttaacgg tcttcgctgt gcctcgtgtc accagattaa
    25441 acagttgtgc gtcacccagt tttgccgcca tggcattcac agccagcaat tcaggatcgg
    25501 ttgccagctg cgcgcgcacc tcatcggcaa cacgctcttt tgccagatcc attttctggc
    25561 gatcagtaag ctgtgcgtgc tgcgggtcgg cgtcaatgga caaaaacgtc tgctgaaatt
    25621 tcggtgactg cgccagctca gagtaatccg cattaagaac agcatctgct gccgccattg
    25681 cactctgccc ctgtgcactg gctgtggaat gggtgatcag gcccgcctgg aataaatccg
    25741 gcattttttt atcgacagct tctgctgcca gtgccgtggc tctttccggc tgcatccctg
    25801 ccgcgatgta ttttttctcc agcccggcgg tcagcatttt tcgcagtgtg acatcaccca
    25861 cctttttagc cacaccgcca gcaaccatat caggtacaag cgcaccaatc aggtttacac
    25921 ccttcgccac ccagaccgca gaatcatcat agccttcggt catcggcgta ttcagcgcac
    25981 gaactgcacc cggcgacatc ttaccggtca gccattcatc agtggcttca gcaccatcac
    26041 tgacagcctt accggttgcc ttcagacctt taccgacagt atcagtaacc gcgtttttgc
    26101 catcaggcag ggtatcgatg acctcatcag cccccctgct gccaccggca aaaatatcct
    26161 gcacagttgt gacacccggc agccccatac ggctgaactc atttaaaata cgcgcccctg
    26221 tttttaccgg gctctgaatc atcgcatcac cgagtccacg ggccatttcc cctgttcccc
    26281 ggacggactg ggcgaaacct ttacccattg ttggcaatac atcgcccagg ctgaacgacg
    26341 tactgttatc cttccagcga ttcggatcag agaaaaatgc ttcatagctg tcagtttcgc
    26401 cgggttgctg aatgttcagg ctgttacgat tctggttacc gagttgcgcc tcaggacgct
    26461 gttcctctga ataggccata cagactccat aaaaaaaccc ggcacaatgg ccgggcatca
    26521 ggaaatggta ttaatggaat atcgtgaatt actgggcgta attctgtttc agtccacgga
    26581 taaactgaga tgcagatgaa gcactttcat ctctggcctg ttcccccctg gccttttgaa
    26641 tacggagaaa attcgcatac ccgttctcca gcaatttctg attctgaggt tccagcattt
    26701 caggctgacg ggccgctaca tttctggcaa atgacagctt atcaggatca tctcccgccc
    26761 agttgataac ctgttgctga agtttttgtt gctgaatttt ctgttgatgc ggttgtgaac
    26821 cagtcgcagc ataatattca tccaccgcag cctgagcatt accgccattc ttgatcgcgt
    26881 ccgccgctac gttgctggcc cccttctgaa gctctttcag agaaagtccc tgctgcttcg
    26941 gcataaaata accataattt ttcgatatct ctgcaaattt gctgcgatcc ctgacctgag
    27001 cgatagcctt atcaacgggt atcgccagca cggtttgatc atcagggtgc gcactgccgt
    27061 attctgttac aggtttatgc gcggtggagc catcgctgta tgtgagatca aggccaataa
    27121 gtacgtaccc ttcctgctgc gccggtacga tactgccaat cctggcctct tttatcgttt
    27181 tttccccggt tgaatcaggc atgccaatac gctgttgtag ttccggggca aacacgccgg
    27241 aaagcacatc gagattttcc ggggtattca gcgaagcaat cgccctgtcc ggcttatcat
    27301 caaagatttt ctgcaggttg ttcacggcct gacccgcttt cgcagcatag cctaccgttg
    27361 acatcatctt aagcggattg gcgtccgata atttaccgaa cagatgagaa gccgcatcat
    27421 ggtcacccgc atcaatcgct tttcccagcg ccgccatcac aggctgatcg cgcgccagca
    27481 tatcgttata ctcaagccgt cgctggttgt atttctgcaa ctggagtcgt tgctgttcca
    27541 tccccagcga cgcattccgg taattctggt tggcgttaaa ctccctttcc tgcagtgtgt
    27601 aattgcggtc atcaactttg gctctgtaat caaagttccg ctgatcaacg tctttattat
    27661 gttcaaactg agactgcgca aattcaaaat cgcgctcgtt atttttctgt tgctgagcaa
    27721 gctgtgcttc tcgcaaacca agctccttac gacgggtcat tgcctggtca acagtgctga
    27781 atccggcaag taacccctgt gcaaatccgc tcattcacca ctccttaaaa caaagcgcct
    27841 gcaatgccgc caattactgc accagcaaca gcgccgacgg ctgtccccac taccggtact
    27901 gctgaaccaa cagtcgcccc gatagtggcc cccgtaccaa tgcccgtacc gatattctgt
    27961 tttttctgag ctttctgttg agcagccagt tgtttatttg atgcctcaat ctctgtacgt
    28021 cgtctgtctg cgtcacttat tccctgtaat gcctcacgcc gcgactgatt tgcaatatcc
    28081 agtaaaccgt atcccatatt gcccccttac gctgccacca gttgtccgcc aacactcagt
    28141 ttctgtcttg ccggtgcaga agcccccgtc agaatattca tctggcgatc ctgttcggct
    28201 tcacggatac catttttcgc acctgctatt gccagggcat tacgtaaccc cagcgtatta
    28261 ctgtcgggat tatccgggcg gcttaccccg tatctcgcca tctggttatc ctgcgccatc
    28321 tgcgctgtac ggagactgga agtggcaagg cctcccaccc gtgcaagctg tggattcatc
    28381 agactgttgt tctccccaag gtcagccagc cttgctacac ggggtaaata tctggtgcgc
    28441 cagtcgtcgt attgctggcg ggtcagcgct gcggatgtct gccagtcccc ctgagggcgg
    28501 gctgctccgg tataaccgta tctcgcacgt gtttcgtatt taccgtactc cataatcaca
    28561 gtctccagtt ctgagcctga tgctgaatgg cattagcgcc agtgccaggt gttttagcac
    28621 cgccacttcc tccgctaccg ccagccttat gcatcgcata tgcacctgcc gcacccagac
    28681 cagcgccaac aagagaggct cgcccctgct gtttcgtaaa cgccgcctgt gcatccgatt
    28741 tagcttttgc cagactgctg tctgccagag agttaaaact ctgtaacgca tccgccttct
    28801 gaccagaacc gagagcagca acatcctgta gcccggcaac atacttatct gcctgcgata
    28861 cctgcccccg tgtggttgtg tcaatctgcc cggttacctg atcactctgg ttcgcattca
    28921 ttaccgcgtt aaagcgacca ctggacgggt caacaccgga ccgagtgaga ttacttgcca
    28981 gctccttccg cgcttcgcca aactgttttt gataccccag atttgtggtg ccagcaatat
    29041 tgtcgtactg ctgctcactg ttcaggtcat cgaccttttc catgaagtta tcttcagccg
    29101 ggcggaggat atttttgtaa tcctgccacc ctttccaggc cacttcttcc tgtgctattt
    29161 gcgctgctgt tggttttact ttggtatcac caccaccttt acttccaccc ataatggccc
    29221 cctggataat aaaaaaaacc tgccggagca gggtcagaat gtgaattgca atgttggttt
    29281 aaacacgatg atgaaaactg tggcagtcag accgctatcc tgaacaccat gaagccatcc
    29341 tcatcatccg gcattcgctc aaagcccaga cgttttccca gccggataaa tccccgcctt
    29401 gccgtatgaa attcagccca acgtccgcca gccagacggg ttaatgtctt cacctccggc
    29461 agataacgct caacactgtt actccccgta cacacgccca gcaacaccag aacataaggg
    29521 ataccatcag cactgagcac agaacgcagc accagaaagc catcaggtgc ctcaaaacaa
    29581 aacgcctgct ttttaaagca ggcgtcttta acttcattca taaattcagg gttgcgggaa
    29641 tttctcacca cacgctgcat ataccggaga attttttcac tcacactctc accacaaaaa
    29701 agaacaatgt gactgtgctg tgtcttcagc gacagaagga tgacggagaa cgcttccgat
    29761 aaccaacacg gctggcgttc tccgcaaaaa ataaattacc agccgacaga catacccgca
    29821 gcaacacctg aaccgttctg ggtatcccat gaagcattca aacgtacagc agaattttca
    29881 gtggtgcgga agtgcatacc tgctgcgata gcctgctcat cacggtagtt acttgcagcc
    29941 ataccaaagg agtacgtttt accctcaaca acaggaatgg cagtcatcgc cattgctcct
    30001 gcaatacctg catctgcacg tttacggttt ttctccacct catcacgtaa ctgacggaat
    30061 tgctgattag tactacgctc cagggtactg acccggttat ctgtgtaggt attagcagac
    30121 ttcagtgtct gtgcatcacc ctgaatacgg gcattctctt ctgcaatacg atagcgctct
    30181 aactgaccat aggttgcggc atccgcagcc tgaacaccat cacgaagccc accaattaca
    30241 cgattcccca catttacttc atcggggcga gtggtctgag agttagcccc tacagcaaca
    30301 ctgttcgtat ggtttgcaat ggcgttgtaa ccgacagcag tggcgttttc tccgcctgct
    30361 gttgccccgc caccaacctg agttttacct gtgcccagag aagacagacg ggcgtgaata
    30421 tctgttacat ctgcagcaga aagaccagtg tcaatagtgg agtctagcgg gtttacagac
    30481 ttcattttaa tttcataaat atattcactg atttcaggaa catgtcttga atacgttttt
    30541 ccatcagcgc ccactgtcgt gaccacgcct tcgccatctg cactaaagag aatttgattg
    30601 gtttttgtgt ttacaaaata agctgtctca ttcgcatagt cataatatga gacaatttca
    30661 ttacttctcg gtgcccgtaa ttctgtattt gttaacccgg actcgccgta atcaaacaga
    30721 gaatatttta aatatttccc tgccgtattt tgtatattta cactatctgg ttgttttgtg
    30781 cctgcattta ccacttttcc atccagatat gcttttcctg agaagccgag gtctttccaa
    30841 aaatcaatta aatttttata ttcatgtcct ttcaactgat caacagtcac tccataatat
    30901 gaagtgccat taattccaga ggcgtctgct gcatatacgc cagcagaaaa taccgacagg
    30961 cttaatgctg gtattaaagc caacaatagc ttgcttgcct ttttcatgat gtgtaccctt
    31021 atgttaaatc atgttataaa tatgttgaca ttcggaacgc aaatgtcttc agggatacta
    31081 ctcataataa caaggcaaat aaaatatcaa aatcaatggg ttaagaaaaa ttaatgatta
    31141 aaatcacaag aatgcaatac gcatcaaaaa cttaccctaa ataacaccag tattactcag
    31201 aaaaaacaaa acattttctg tggttattat ttaccattga taaatattac ctctctctgt
    31261 cagcctgcat tgtataaaac atacactaca aacgggtgcc atatcggctg aacctcagca
    31321 accagttgac ggggactttc gtccccgtcg cggttttcct actgcttaca ctgtaagaac
    31381 gccgcaaact ccgctcccca caaattcagc cggaactcac acagcgaacc gtgcaacatc
    31441 cagatagtga agataaccgt catacaaatt gtgacggtga taagcgattt ttgcgacata
    31501 gcgcttgact ccttttacag agaggcgcta accttctact tgcttaaggt atgatgatta
    31561 gggcctcggg ttaacgttaa gttgactcgg ggcctttcca catcaggcct tcaggttctc
    31621 cctccagcca tcagccgaaa ggcacccacg cataatttac ggtttttgcc ctgtacgggc
    31681 aataaaaaac ccgccatcac agcgggtagt aaacggttac cggatacagg tcagaatttc
    31741 aggccaatgc cagcagtaac gccactcgtg cgccagtcgc cagagccgga accttcgtaa
    31801 gccacatcca gtgtaacact ctcattcagg ttaaactgtg cacctgcagc ccacgcaagg
    31861 gaggtttttt tcgtgctgtt gctttcagaa aatccgccac tactgttaat attgtcctta
    31921 attttcaggt cagcgctaac tttagcaacg cccatccctg ccatcgcata taaactcatg
    31981 tactgattaa cccgccatga aggccccgct aacagactcc agtaattcgc cctgatatcc
    32041 gttctggcag aagctgccgg attcttaatc ttcctggtat gatcggctga ctgcacatca
    32101 ataaatgtct gtgagttagt gagagaacgc gtccatgtaa aagaggtgat aacgccaaaa
    32161 tcatccgtta tctcgtagcg atacctgata ttaatgccct gtggattttt atccttgcct
    32221 tcatatccac tgacccgacc atctgtatat tcgctcaatg aagaaaagta gtttctgttg
    32281 acgaaatgac tgaaagtctc cctgttatga gcagtcgcat cctttacaaa atccttcagt
    32341 cccggaaact gaaagtgcgc ataccccaga gaaattgtat ggtttccttc tgctgcatgt
    32401 gcagataaat ttacacaggc aatcccggag attgcacaca caaccagtgt tgctatactc
    32461 ttcatttaag atatcctgta ttcagataac acgcta
//
LOCUS       DAZFRN010000042        32384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFRN010000042 DAZFRN010000000
VERSION     DAZFRN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.042"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            58..1347
                     /gene="hpaG"
                     /locus_tag="ACHKUB_001901"
     CDS             58..1347
                     /gene="hpaG"
                     /locus_tag="ACHKUB_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO6654925.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVGEEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1344..2810
                     /gene="hpaE"
                     /locus_tag="ACHKUB_001902"
     CDS             1344..2810
                     /gene="hpaE"
                     /locus_tag="ACHKUB_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6654926.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2812..3663
                     /gene="hpaD"
                     /locus_tag="ACHKUB_001903"
     CDS             2812..3663
                     /gene="hpaD"
                     /locus_tag="ACHKUB_001903"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO6654927.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3673..4053
                     /locus_tag="ACHKUB_001904"
     CDS             3673..4053
                     /locus_tag="ACHKUB_001904"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO6654928.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4121..4924
                     /gene="hpaH"
                     /locus_tag="ACHKUB_001905"
     CDS             4121..4924
                     /gene="hpaH"
                     /locus_tag="ACHKUB_001905"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO6654929.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHIDYGNMGSISCRFV"
     gene            4935..5723
                     /gene="hpaI"
                     /locus_tag="ACHKUB_001906"
     CDS             4935..5723
                     /gene="hpaI"
                     /locus_tag="ACHKUB_001906"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO6654930.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5898..7274
                     /gene="hpaX"
                     /locus_tag="ACHKUB_001907"
     CDS             5898..7274
                     /gene="hpaX"
                     /locus_tag="ACHKUB_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO6654931.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVNNATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7284..8174
                     /gene="hpaA"
                     /locus_tag="ACHKUB_001908"
     CDS             7284..8174
                     /gene="hpaA"
                     /locus_tag="ACHKUB_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO6654932.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8425..9987
                     /gene="hpaB"
                     /locus_tag="ACHKUB_001909"
     CDS             8425..9987
                     /gene="hpaB"
                     /locus_tag="ACHKUB_001909"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO6654933.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10005..10517
                     /locus_tag="ACHKUB_001910"
     CDS             10005..10517
                     /locus_tag="ACHKUB_001910"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO6654934.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGNLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10910..13060
                     /gene="btsT"
                     /locus_tag="ACHKUB_001911"
     CDS             10910..13060
                     /gene="btsT"
                     /locus_tag="ACHKUB_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO6654935.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13110..13313
                     /gene="yjiX"
                     /locus_tag="ACHKUB_001912"
     CDS             13110..13313
                     /gene="yjiX"
                     /locus_tag="ACHKUB_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6654936.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13324..14280
                     /gene="yjiA"
                     /locus_tag="ACHKUB_001913"
     CDS             13324..14280
                     /gene="yjiA"
                     /locus_tag="ACHKUB_001913"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO6654937.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14358..16898)
                     /locus_tag="ACHKUB_001914"
     CDS             complement(14358..16898)
                     /locus_tag="ACHKUB_001914"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFO6654938.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMTFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17250..17414)
                     /gene="yjiS"
                     /locus_tag="ACHKUB_001915"
     CDS             complement(17250..17414)
                     /gene="yjiS"
                     /locus_tag="ACHKUB_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO6654939.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17591..19003
                     /gene="yjiR"
                     /locus_tag="ACHKUB_001916"
     CDS             17591..19003
                     /gene="yjiR"
                     /locus_tag="ACHKUB_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6654940.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQSYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(19245..20189)
                     /locus_tag="ACHKUB_001917"
     CDS             complement(19245..20189)
                     /locus_tag="ACHKUB_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6654941.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            20656..21888
                     /gene="mdtM"
                     /locus_tag="ACHKUB_001918"
     CDS             20656..21888
                     /gene="mdtM"
                     /locus_tag="ACHKUB_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO6654942.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21929..23209
                     /gene="yjiN"
                     /locus_tag="ACHKUB_001919"
     CDS             21929..23209
                     /gene="yjiN"
                     /locus_tag="ACHKUB_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO6654943.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRYAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            23325..24476
                     /gene="yjiM"
                     /locus_tag="ACHKUB_001920"
     CDS             23325..24476
                     /gene="yjiM"
                     /locus_tag="ACHKUB_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO6654944.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24486..25253
                     /gene="yjiL"
                     /locus_tag="ACHKUB_001921"
     CDS             24486..25253
                     /gene="yjiL"
                     /locus_tag="ACHKUB_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO6654945.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25250..25507
                     /gene="ytiA"
                     /locus_tag="ACHKUB_001922"
     CDS             25250..25507
                     /gene="ytiA"
                     /locus_tag="ACHKUB_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO6654946.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            25572..26432
                     /gene="yjiK"
                     /locus_tag="ACHKUB_001923"
     CDS             25572..26432
                     /gene="yjiK"
                     /locus_tag="ACHKUB_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO6654947.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26500..27678
                     /gene="yjiJ"
                     /locus_tag="ACHKUB_001924"
     CDS             26500..27678
                     /gene="yjiJ"
                     /locus_tag="ACHKUB_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6654948.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27691..28245)
                     /gene="kptA"
                     /locus_tag="ACHKUB_001925"
     CDS             complement(27691..28245)
                     /gene="kptA"
                     /locus_tag="ACHKUB_001925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO6654949.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28495..29178
                     /gene="yjiH"
                     /locus_tag="ACHKUB_001926"
     CDS             28495..29178
                     /gene="yjiH"
                     /locus_tag="ACHKUB_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO6654950.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29175..29636
                     /gene="yjiG"
                     /locus_tag="ACHKUB_001927"
     CDS             29175..29636
                     /gene="yjiG"
                     /locus_tag="ACHKUB_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO6654951.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29649..30821
                     /gene="iadA"
                     /locus_tag="ACHKUB_001928"
     CDS             29649..30821
                     /gene="iadA"
                     /locus_tag="ACHKUB_001928"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6654952.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVNGTFETA"
     gene            30886..31797
                     /gene="hypT"
                     /locus_tag="ACHKUB_001929"
                     /gene_synonym="qseD"
     CDS             30886..31797
                     /gene="hypT"
                     /locus_tag="ACHKUB_001929"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO6654953.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31790..32182)
                     /gene="iraD"
                     /locus_tag="ACHKUB_001930"
     CDS             complement(31790..32182)
                     /gene="iraD"
                     /locus_tag="ACHKUB_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO6654954.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(32179..32262)
                     /gene="idlP"
                     /locus_tag="ACHKUB_001931"
     CDS             complement(32179..32262)
                     /gene="idlP"
                     /locus_tag="ACHKUB_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO6654955.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         7420 a         8529 c         8500 g         7935 t
ORIGIN      
        1 aataaaagta atcattaaca tattaatgat taaaatgatc gaatccgagg agtggtaatg
       61 aaaggcacta tcttcgccgt agcgttgaac catcgcagcc aacttgatgc atggcaggaa
      121 gcgttccagc aatcccccta caaagccccg cctaaaactg cggtctggtt tattaaaccg
      181 cgcaatacgg tgattggttg cggtgaaccg attccctttc cacagggtga aaaggtactg
      241 agcggtgcga ctgttgcgct gattgtggga aaaacggcga cgaaagtagg tgaagaagat
      301 gcggcagagt acatcgccgg atatgcgctg gctaacgacg tcagcctgcc ggaagagagc
      361 ttttaccgcc cggcaatcaa agcaaaatgt cgtgatggat tctgccccat tggcgaaacc
      421 gtggctctca gcaatgtcga taatctgacc atctataccg agatcaacgg gcgtcctgcc
      481 gatcactgga ataccgccga tttacaacgt aacgccgcgc agttgctgag cgccctgagc
      541 gaatttgcca cactgaatcc aggcgatgcc attctgctcg gcacgccaca ggcgcgcgtg
      601 gaaatacagc caggcgatcg cgttcgtgtt ctcgcagaag gtttcccgcc gctggaaaat
      661 ccggtagtgg acgaacgtga agtgaccacg cgcaagagct tcccaacgca gccacacccg
      721 cacggcacgc tgtttgccct cggcctgaac tacgccgacc acgccagcga actggaattt
      781 aagccaccgg aagaaccgct ggtgttcctg aaagcgccaa ataccctcac tggcgataac
      841 cagacctccg tgcgtccaaa caatattgaa tacatgcact atgaagcgga gctggtggta
      901 gttattggca aacaggcgcg taacgtcagc gaagccgatg ccatggatta tgtcgcgggc
      961 tacaccgtgt gtaacgacta cgccattcgc gactatctgg aaaactacta ccgccctaac
     1021 ctgcgggtca aaagccgcga cggactgacg ccgatgcttt caaccatcgt gccgaaagag
     1081 gcgatcccgg acccgcataa tctgaccctt cgcaccttcg tcaacggcga gttacgccag
     1141 caaggcacca ccgccgatct gatcttcagc gtgcccttcc tgatcgccta cttaagcgaa
     1201 tttatgaccc tgaatccggg cgacatgatc gccaccggca caccaaaagg cttatctgac
     1261 gtggtgcctg gcgatgaagt ggtggtggaa gtagaaggcg tgggccgcct ggtgaaccga
     1321 attgtgagtg aggaaacagc gaaatgaaaa aagtaaatca ttggatcaac ggtaaaaatg
     1381 ttgcaggtaa cgactacttc cagactacca atccggcaac gggtgaagtg ctggcggatg
     1441 tggcctccgg cggtgaagcg gagatcaatc aggcggtagc ggcagcgaaa gaggcgttcc
     1501 cgaaatgggc caatctgccg atgaaagagc gcgcgcgcct gatgcgccgt ctgggcgatc
     1561 tgatcgacca gaacgtgccg gagatcgccg cgatggaaac cgcggacacg ggcctgccga
     1621 tccatcagac caaaaatgtg ttgatcccac gcgcttctca caactttgaa tttttcgcgg
     1681 aagtctgcca gcagatgaac ggcaagactt atccggtcga cgacaagatg ctcaactaca
     1741 cgctggtgca gccggtaggc gtttgtgcac tggtgtcacc gtggaacgtg ccgtttatga
     1801 ccgccacctg gaaggtcgcg ccgtgtctgg cgctgggcaa taccgcggta ctgaaaatgt
     1861 cggaactctc cccgctgacc gccgaccgcc tgggtgagct ggcgctggaa gctggtattc
     1921 cggcgggcgt gctgaacgtg gtacagggct acggcgcaac cgcaggcgat gcgctggttc
     1981 gtcatcatga cgtgcgtgcc gtgtcgttca ccggcggtac cgccaccggg cgcaacatca
     2041 tgaaaaacgc cgggctgaaa aaatactcca tggaactggg cggtaaatcg ccggtgctga
     2101 tttttgaaga tgctgatatt gaacgcgcgc tggacgccgc cctgttcacc atcttctcga
     2161 tcaacggcga acgctgcacc gccggttcgc gcatctttat tcagcagagc atctacccgg
     2221 aattcgttaa acgctttgcc gaacgcgcca accgcctgcg cgtgggcgat ccgaacgatc
     2281 cgaataccca ggttggcgcg cttatcagcc agcagcactg ggaaaaagtc tccggctata
     2341 tccgtctcgg cattgaagaa ggcgcaaccc tgctggcggg cggcccggat aaaccgtccg
     2401 acctgcctgc gcacctgaaa ggcggcaact ttctgcgccc aaccgtgctg gcagacgttg
     2461 ataaccgtat gcgcgtcgcc caggaagaga ttttcgggcc ggtcgcctgc ctgctgccgt
     2521 ttaaagacga agctgaaggc ttacgcctgg caaacgacgt ggagtacggc ctcgcgtcgt
     2581 acatctggac tcaggatgtc agcaaagtgc tgcgtctggc gcgtggcatt gaagctggca
     2641 tggtgttcgt caacacccag aacgtgcgtg atttgcgcca gccgtttggc ggcgtaaaag
     2701 cctccggcac cgggcgtgaa ggcggtgagt acagcttcga agtgttcgcg gaaatgaaga
     2761 acgtctgcat ttccatgggc gaccatccaa ttccgaaatg gggagtctga tatgggtaag
     2821 ttagcgttag ccgccaagat cacgcacgta ccgtcgatgt atctctctga actgccaggg
     2881 aaaaaccacg gttgccgcca gggcgcgatc gacgggcata aagagatcag caagcgttgc
     2941 cgggaaatgg gcgtcgatac cattatcgtt ttcgataccc actggctggt caacagtgct
     3001 tatcacatca actgtgcaga ccattttgaa ggcgtctaca ccagcaacga gctaccgcat
     3061 tttatccgcg atatgaccta caactacgag ggcaacccgg agttggggca gcttattgcc
     3121 gatgaagcct taaagctcgg cgtgcgggca aaagcgcaca acattcccag cctgaaactg
     3181 gaatacggca cgctggtgcc aatgcgctac atgaatgaag acaagcactt caaagtggtt
     3241 tccatttcag ctttctgcac tgttcacgat tttgccgaca gccgcaagct gggcgaggcg
     3301 attctgaaag cgatcgaaca gtacgacggc accgtggcgg tccttgccag cggttcgtta
     3361 tcgcaccgct ttattgacga tcagcgagcg gaagaaggga tgaacagcta cacccgcgag
     3421 ttcgaccgcc agatggacga gcgcgtggtg aagctgtggc gcgaaggcca gttcaaagag
     3481 ttctgcaata tgctgccgga gtacgccgac tactgctacg gcgaaggcaa tatgcacgac
     3541 acggtgatgc tgctggggat gctcggctgg gataaatacg acggcaaggt ggagtttatc
     3601 accgagctat tcccaagctc tggcaccggt caggttaacg ctgttttccc gcttcccgcg
     3661 taaggacgtt ttatgccgca ctttatcgtg gaatgtactg aaaatattcg tgaagaagcc
     3721 cgcttacctg agctgtttgc cagtgttaac actgcactgg cggcaacggg gatttttccg
     3781 cttgggggaa tacgtagccg ggcgcactgg atcgatacct ggcaaatggc tgatggcaag
     3841 cacgattacg cctttgtgca tatgacgcta aaaatcggca gcgggcgcag cctggaaagc
     3901 cgtcaggaag tgggcgaaat gctgtttgat ctgattaaaa cgcacttcgc ctcactgatg
     3961 gagagccgtt acctggcgct ttcatttgag attgcagagc tgcacccgac gctgaatttt
     4021 aaacaaaaca acgtgcacgc gttgtttaaa taaatttgca ccagcttcca ggccggatga
     4081 taaggcgcag ccgcatccgg caatgccaca ggatatcgct atgttcgata aacacaccca
     4141 caccctgatc gcccagcgtc tggatcaggc agaaaaacag cgcgaacaga tccgcgcgat
     4201 ctcgctggat tacccggaga tcaccatcga agacgcttac gcggtgcagc gtgaatgggt
     4261 tcgactgaaa atcgccgaag gtcgcacgct gaaaggccac aaaatcggcc tgacctcgaa
     4321 agcgatgcag gccagctcac aaatcagcga gccggattac ggtgccctgc tggatgacat
     4381 gttcttccac gatggcagcg atatcccgac cgatcgcttt atcgtgccgc gcattgaagt
     4441 ggagctggct ttcgtgctgg caaaaccgct gcgtggacca aactgcacgc ttttcgacgt
     4501 ctacaacgcc acggactacg tgatcccggc gctggagctg atcgacgctc gctgccacaa
     4561 catcgatccg gaaacccagc gcccgcgcaa agtgttcgac accatttctg ataacgccgc
     4621 caacgccggg gtgatcctcg gtggtcgtcc cattaagccc gatgagttgg atctgcgttg
     4681 gatctccgcc ctgatgtatc gcaatggcgt gattgaagaa accggcgtcg ccgctggtgt
     4741 gctaaatcat ccggcaaacg gcgtggcctg gctggcgaac aaactcgcac cgtatgacgt
     4801 acaactggaa gccgggcaaa tcattctcgg cggttcgttc acccgcccgg ttccggcgcg
     4861 taagggcgac accttccaca tcgattacgg caacatgggc tccattagct gccgctttgt
     4921 ttaaggagag aacgatggaa aacagtttta aagcggcgct gaaagcaggc cgcccgcaga
     4981 tcggattatg gctggggctg agtagcagct acagcgcaga gttactggcc ggagcaggat
     5041 tcgactggtt gttgatcgac ggtgagcacg cgccgaataa cgtgcaaacc gtgctcaccc
     5101 agctacaggc gattgcgccc taccccagcc agccggtagt acgtccgtcg tggaacgatc
     5161 cggtgcaaat caaacaactg ctggacgtcg gcacacaaac cttgctggtg ccgatggtac
     5221 aaaacgctga cgaagcccgt gaagcggtac gcgccacccg ttatcccccc gccggtattc
     5281 gcggtgtggg cagtgcgctg gcccgcgcct cgcgctggaa tcgtattcct gattacctgc
     5341 aaaaagccaa cgatcaaatg tgcgtgctgg tgcagatcga aacgcgtgag gcaatgaaga
     5401 acttaccgca gattctggac gtggaaggcg tcgacggcgt gtttatcggc ccggcggatc
     5461 tgagcgccga tatgggttat gccggtaatc cgcagcaccc ggaagtacag gccgccattg
     5521 agcaggcgat cgtgcagatc cgtgaatcgg gcaaagcgcc ggggatcctg atcgccaatg
     5581 agcaactggc aaaacgctat ctggaactgg gcgcgctgtt tgtcgccgtc ggcgttgaca
     5641 ccaccctgct cgcccgcgcc gctgaagcgc tggcagcacg gtttggtgcg caggccaccg
     5701 ccgtgaagcc cggcgtgtat taatgccgga tgcgctgcac ttatccggcc tacactcgca
     5761 ccaaaccgta ggccggataa ggcgtttacg ccgcatccgg caaaaagccg taccaaatcg
     5821 cggaccggat aaggcgtttg tgcgcatccg ccataaacct tgtcgtaccc tacaaaaatc
     5881 ccattagagg aagaaaaatg agcgacacct cacctgccat accggagagt atcgatccgg
     5941 cgaatcagca taaagcgctg actgccggac aacaggcggt tattaagaag ctatttcgcc
     6001 gcctgatcgt ctttctgttc gtgctgttta tcttctcgtt ccttgatcgc atcaacatcg
     6061 gctttgccgg actcacgatg ggacgcgacc tcggtctgag cgccaccatg tttggcctcg
     6121 ctaccaccct gttctatgcc gcttatgtca tcttcggcat tcccagcaac attatgctga
     6181 gtattgtcgg tgcgcggcgc tggatcgcca ccatcatggt gctctggggc atcgcctcta
     6241 ctgccaccat gtttgccact ggccccacca gcttgtacgt actgcgtata ctggttggca
     6301 ttaccgaagc cggctttctg cctggtattc tgctgtattt aaccttctgg tttccggcct
     6361 atttccgcgc ccgtgccaac gccttgttta tggtagcaat gccggtaacg acagcgttgg
     6421 gctcgatcgt ttccggctac attttgtcgc tggatggcgt catggcatta aaaggctggc
     6481 agtggctgtt tttgctggaa ggcttcccgt cggtattact cggcgtcatg gtgtggttct
     6541 ggcttgatga ctcaccggac aaagctaagt ggctgacgaa agaagacaaa aaatgcctgc
     6601 aagagatgat ggataacgat cgtctgacgc tggttcagcc agagggagcc atcagccacc
     6661 acgccatgca acaacgcagt atgtggcggg agatcttcac tccggtggtg atgatgtata
     6721 ccctggcgta tttctgcctg accaacacac ttagtgcgat cagcatctgg acaccgcaga
     6781 tcctgcaaag ttttaatcag ggcagcagta atatcaccat cggcctgctg gccgccgtac
     6841 cgcagatttg taccattctc gggatggtct actggagccg tcactcagat cgccgccagg
     6901 aacgaaggca tcacaccgcc cttccttatt tgttcgctgc cgcaggttgg ttactggctt
     6961 cggcaactga tcacaacatg atccagatgc tggggatcat tatggcttcg accggatcat
     7021 tcagcgcaat ggcgattttc tggacaacac cggatcagtc catcagcctg cgggcacgag
     7081 cgatcggtat tgcggtgaac aacgccactg gcaacattgg ttcagcgtta agtccgttta
     7141 tgatcggctg gttgaaagat ttgaccggca gctttaacag tggattgtgg tttgttgccg
     7201 cgctgctggt gattggtgcg gggattatct gggcgattcc aatgcagtcc tcccgtccgc
     7261 gagcgacccc gtaaggaacg acgatgtgtg accgtcagat tgccaatatt gatatcagca
     7321 aagagtacga tgaaagcctg ggcacggacg atgtgcatta tcagtcgttc gcccgcatgg
     7381 cggccttttt tggccgccat atgctgccac atcgccacga acagtacttt cagatgcatt
     7441 tcctcaatag cggacagatt gagctacagc ttgacgatca tcgctactcg gtggaagcgc
     7501 ccctgtttgt cctgacgccg ccgtcagtac ctcatgcgtt tattacggag tctgatgctg
     7561 acggtcatgt attgacggta cgggaagatc tgatctggcc cctgctggaa gttctttatc
     7621 cgggcactcg ggaaaccttc ggcctgccgg ggatttgcct gtcactggca gataaacccg
     7681 acgaactggc ggcgctggaa cactattggc aactgataga gcgggaatcg gtagaacaac
     7741 tgcctggacg ggaacacacc ctgacgttac tggcacaggc agtgttcacc ctactgctgc
     7801 gtaacgcaaa actcgacgac catgccgcca gcggaatgcg cggagaatta aaactgttcc
     7861 agcgttttca tatgcttatt gaaagccatt ttcatcagca ctggacagta ccggattacg
     7921 ctaacgaact gcatatcacc gaatcacgcc tcacggacat ctgccgccgc tttgccaacc
     7981 gtccgccaaa acggttgatt ttcgacaggc agctacgaga agccaagcgg ctgctgctgt
     8041 tttctgataa cgccgtgaac aatattgcct ggcaactcgg ttttaaggat ccggcttatt
     8101 ttgcgcgctt ttttaatcgc ttagtcggtt gctcgcccag tgcttatcgt gccaaaaaag
     8161 tacctgtgac gtgaaattcc cttagcctta acgggaaacc aggcaccacc tgctattccc
     8221 ctttcttcgc ctggagcaag gggatttagt aacgttaaaa atcgctaaaa gcgacctcga
     8281 tcacaaaacc gtacccgacc tgaaaagtac cagccattgt cccaaaagtg tcttcccgta
     8341 atcgcccctg caagcttcaa ttgaataaca aaaacaacac acaaaattaa caatcgtatt
     8401 ccgattaata ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt
     8461 ccgttcaccg gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat
     8521 ggcgagcgag tgaaagacgt caccactcat ccggcatttc gtaatgcggc agcgtctgtt
     8581 gcccaactgt acgacgcgct acacaaaccg gagatgcagg actctctgtg ctggaacacc
     8641 gacaccggta gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac
     8701 ctgcgccagc aacgcgacgc catcgctgag tggtcacgcc tgagctatgg ctggatgggc
     8761 cgtaccccag actacaaagc cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac
     8821 ggtcagttcg agcagaacgc ccgtaactgg tatacccgta ttcaggaaac tggcctctac
     8881 tttaaccacg cgattgttaa cccaccgatc gatcgtcatt tgccgaccga taaagtgaaa
     8941 gacgtttaca tcaagctgga aaaagagact gacgccggga ttatcgtcag cggtgcgaaa
     9001 gtggttgcca ccaactcggc gctgactcac tacaacatga ttggcttcgg ctcggcacaa
     9061 gtgatgggcg aaaacccgga cttcgcactg atgttcgttg cgccaatgga tgccgatggc
     9121 gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg ctcgccgtac
     9181 gactacccgc tctccagccg ctttgatgag aatgatgcga ttctggtgat ggataacgtg
     9241 ctgatcccat gggaaaacgt actgatctac cgcgatttcg atcgctgccg tcgctggaca
     9301 atggaaggcg gttttgcccg tatgtatccg ctgcaagcct gtgtgcgcct ggcagtgaaa
     9361 ctcgacttca ttacagcact gctgaaaaaa tcactcgaat gtaccggcac cctggagttc
     9421 cgtggtgtac aggccgatct cggtgaagtg gtggcgtggc gcaacacctt ctgggcattg
     9481 agtgactcga tgtgttctga agcgacgccg tgggtcaacg gggcttattt accggatcat
     9541 gccgcactgc aaacctatcg cgtactggca ccaatggcct acgcgaagat caaaaacatt
     9601 atcgaacgca acgttaccag tggcctgatc tatctccctt ccagtgcccg tgacctgaac
     9661 aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg gttcgaatgg tatggatcac
     9721 gtccagcgca tcaagatcct caaactgatg tgggacgcca ttggcagcga gtttggtggt
     9781 cgccacgaac tgtatgaaat caactactcc ggtagccagg atgagattcg cctacagtgt
     9841 ctgcgccagg cacaaagctc cggcaatatg gacaagatga tggcgatggt tgatcgctgc
     9901 ctgtcggaat acgaccagaa cggctggact gtgccgcact tgcacaacaa cgacgatatc
     9961 aacatgctgg ataagctgct gaaataacgc agcaggaggt taagatgcaa ttagatgaac
    10021 agcgcctgcg ctttcgtgac gcgatggcca gcctgtcggc agcggtaaat attatcacca
    10081 ccgagggcga cgccggacaa tgcgggatta cggcaacggc cgtctgctcg gtcacggata
    10141 caccaccatc gctgatggtg tgcattaacg ccaacagtgc gatgaacccg gtttttcagg
    10201 gcaacggcaa tttgtgcgtc aacgtcctca accatgagca ggaactgatg gcacgccact
    10261 tcgcgggcat gacgggcatg gcgatggaag agcgttttag cctctcatgc tggcaaaaag
    10321 gtccgctggc gcagccggtg ctaaaaggtt cgctggccag tcttgaaggt gagatccgcg
    10381 atgtgcaggc aattggcaca catctggtgt atctggtgga gattaaaaac atcatcctca
    10441 gtgcagaagg tcacgggctt atctacttta aacgccgttt ccatccggtg atgctggaaa
    10501 tggaagctgc gatttaagct ccagcccctt cacgtattcg tcgaaggggc atttttattg
    10561 tgatctcaat cacatatcca cccgatgaat aattcttgcc caaccgctca ccgccattcc
    10621 tgaccgctta cgtccctaaa ataaccactc agttatttac cttactttac gcgcgcgtaa
    10681 ctctggcaac atcactgcag gatagcggtc aatttacctc ctcaaacgca acgcaaacct
    10741 agaacggctt cggcctatga ttaacaaatt actctatgaa ttttggtttg caggaaggca
    10801 gcaagtgagt gaatccccgg gagcttacaa cagtaagtga caggggtgaa cgaacgcaac
    10861 tgccgcacct gtaagccaaa agacgacgag taaaatgcca ggttttacta tggatacgaa
    10921 aaaactattc aagcacatac cctgggtgat tctcggaatc atcggtgcat tctgcctcgc
    10981 ggtagttgca ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt
    11041 gtcggtgtat ctggtggcgt atcgctacta cagtctgtac atcgcccaga aggtgatgaa
    11101 actcgacccc acgcgcgcga cgcctgcggt tattaacaac gacggtctga actacgttcc
    11161 gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct atcgccggtg ctggtccgct
    11221 ggttggtccg gttctcgccg cgcagatggg ctacctacct ggcacgctgt ggctgctggc
    11281 gggggtagta ctggccggtg cggtacagga ctttatggtg ctgtttatct cctctcgccg
    11341 taacggcgca tctctcggtg agatgatcaa agaagagatg ggaccagtac cggggactat
    11401 cgcgctgttt ggctgtttct taatcatgat catcatcctt gccgtcctgg cgctgattgt
    11461 ggttaaagcc ctggccgaaa gcccgtgggg tgttttcacc gtttgctcaa ccgtaccgat
    11521 tgcgctgttt atgggtatct acatgcgctt tatccgtccg gggcgtgtgg gtgaagtctc
    11581 tgtcattggt atcgtgctgc tggttgcctc tatctacttc ggtggcgtga ttgctcacga
    11641 tccgtactgg ggtccggcac tgacctttaa agacaccacc attaccttcg cgctgattgg
    11701 ctatgcgttt gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc gcgactatct
    11761 ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg ctgggtattg tggtgctgaa
    11821 cccggaactg aaaatgcctg cgatgaccca gtacattgac ggtactggcc cgctgtggaa
    11881 aggcgctctg ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat ctggcttcca
    11941 cgcgctgatc tcttccggta cgacgccgaa actgctggct aacgaaaccg acgcgcgttt
    12001 cattggctac ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc tggttgctgc
    12061 gtccatcatc gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg gccttggcat
    12121 taccatgcct aacctgcatg aaatgggtgg cgagaatgcg ccgatcatca tggcgcagct
    12181 gaaagacgtt accgcacacg cggcggcaac cgtcagctcc tggggcttcg tgatttcgcc
    12241 agagcagatc ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga accgtgcagg
    12301 tggtgcgcca acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc tgccgatggc
    12361 tgacatgggc ttctggtatc acttcggtat tctgttcgaa gccctgttca tcctgaccgc
    12421 gctggatgcg ggtacccgtt ctggccgttt tatgctgcaa gacctgctgg gtaacttcat
    12481 cccgttcctg aaaaaaaccg actctctggt tgccggtatc atcggtactg cgggctgtgt
    12541 gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag
    12601 cctgtggccg ctgttcggta tttccaacca gatgctggca gccgtagcgc tggtactggg
    12661 caccgttgtg ctgattaaga tgaagcgcac ccaatacatc tgggtaactg ttgttccggc
    12721 tgtatggctg cttatctgca ccacctgggc gctgggtctg aaactgttca gcaccaaccc
    12781 gcagatggaa ggcttcttct acatggcaag ccagtacaaa gagaagattg ctaacggtac
    12841 tgacctgacg gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca actacaccaa
    12901 cgcaggcctg agtattctgt tcctgattgt ggtgtacagc atcatcttct acggtttcaa
    12961 aacctggctt gcggtacgta acagcgacaa acgtactgac aaagaaactc cgtacgttcc
    13021 aatcccggaa ggcggcgtga agatctcttc gcaccactaa ccgtgtttag ccccgcttcg
    13081 gcggggcttt gttcttatca gagtgaacta tgtttggtaa cttaggacag gcaaaaaaat
    13141 atctcggcca ggcggcgaag atgttgattg gcattccaga ctatgacaac tacgtcgagc
    13201 atatgaagac caaccatccc gacaagccgt acatgagcta tgaagaattc ttccgcgaac
    13261 gccagaacgc acgctacggc ggcgatggta aaggcggtat gcgctgttgt taatggagag
    13321 agcatgaacc cgattgcagt taccctactc accggttttt taggcgcggg caaaaccacc
    13381 ctgctgcgcc atattcttaa tgaacaacac ggctacaaga ttgccgtgat tgaaaacgaa
    13441 ttcggcgaag tctccgttga tgatcaattg attggtgatc gcgcaacgca gatcaaaacg
    13501 ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc gctactcgac
    13561 ctgctggata atctcgacaa gggcaatatt cagttcgatc gtctggtcat tgaatgcacc
    13621 ggcatggccg atcctggccc gattattcaa acctttttct cccatgagat tttatgccag
    13681 cgctacctgc tggacggcgt gattgcgctg gtggatgcgg tacatgccga tgagcagatg
    13741 aaccagttca ccatcgccca gtcccaggtg ggctacgccg accgtattct gctgactaaa
    13801 accgacgtcg caggcgaagc agaaaaactg cgtgaacgtc tggcacgcat caacgcccgt
    13861 gcaccggtct acaccgtcac ccacggcgac atcgacctgg gtctgctgtt caacaccaac
    13921 ggttttatgc tggaagaaaa cgtcgtcagc accaaaccgc gtttccattt tatcgcggac
    13981 aaacaaaacg atatttcgtc gattgtggtg gaactggatt acccggtaga tatcagcgaa
    14041 gtttcccggg tgatggaaaa cctgctgctg gaatcggcgg ataaactgct gcgttacaaa
    14101 gggatgctgt ggattgacgg cgaacctaac cgcctgctgt tccagggcgt ccagcgcctc
    14161 tacagcgccg actgggacag gccatggggc gatgaaacac cgcatagcac gatggtgttt
    14221 atcgggattc agttgccgga ggatgagatt cgggcggcgt ttgcgggact caaaaagtaa
    14281 ccagccctcc aacatcacaa tacctgagac tttatcaccc tcatattcct ccagaatatg
    14341 agggctttcg ggcttaatta tctctgaacg tgcaacactt cctgcgactc cttatgcgcc
    14401 tccacatcct gcttcgttaa ccagagcgac ttacggccaa aattttcgta cattttcagc
    14461 tgatcttcat agtgcttatc aactgttcca tcgggagcaa taaacccact ctgaccgggt
    14521 gcgaccacat cccaggcaag cacaggacga tcgcttgtcg ttggtgagaa aacaatcata
    14581 tcgttttctg ttccacggtt ttgatactcc gcctgatgac gcgtttcttc cgctgcggcc
    14641 tgcggtacac caaagaaatt atttgcccgg aacgttaagg ccattgcagg tgttttccag
    14701 ttactcacat tattgccata gcgtttggaa agagtctccc aggtatcttc cagcgcagcc
    14761 aacacaacct cctgctgtgg tttcccagca aacagatcaa ccgcctgtgg gattggtgat
    14821 ttgtctccct gcaccgcctc atacaaaatt tttgctccaa cacttatatt cagcgaacca
    14881 gttgggccgt cctgggttgt ttcgtagcca ctggcgctgt accacttatc aaatggcata
    14941 ggtacggcag ccactacggt acgcttcaac atactggtca gccaaacgtt caggatggca
    15001 gagcctggct gctgccaggt tttaccatca tcattaagca aattgatgcc atcccaacgt
    15061 gttaatgttt ctaccaactg acgacgcgga tcgctctgtg tcaaaccaga tgtcgctgct
    15121 tgcagagtag gtaaaaaaag cctcaggtta agatcctgac gactggtttg gcgaataaca
    15181 tcccatgcct gatcagcagt taagcgtggc ttttgctcaa gcagtcggtc gatctccgta
    15241 acgcgatctg caccacccca caaaaaggca aacagatctg aagcgggata atctttttgg
    15301 ggagaattgt tccagttagc aatatatccc gactgggggt tatacacctt agggttcatt
    15361 tcaaaaggca atagcccttt ccagtcccat tttcccgtac caggaacggg taatcgcgga
    15421 tcatggcctg attgacgatc tggataagca ccagtatgaa cataaccaat attgccgttt
    15481 acatcagcat agtaccagtt gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc
    15541 tgccaatttt tggccttcat ctgatgagtc caggccagca aagacgccac ctctttacca
    15601 tcccatgcgc gggatttagc gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg
    15661 ccatgcaccg tacgccagac agtaaaggtc tctgcctgac catttttcac cgtaatggtt
    15721 tcctcacggc ttaacatttt cacccactta ccattatgca agtagtagcc tggtttctct
    15781 gccgacagcc gttcagcaaa aatatcgaca tcatcgccga aacctgccgt tgatccccag
    15841 gaaatcacac cattatgacc aaaaaccagc ccaggatagg caaatggtgt attgccagtg
    15901 acatcataac cagcaccgtg cagaccaata ccataagtat acgcaggcgc ataccagcca
    15961 aactgcggac catttaccat gattgctttc gcatcctggg ctttgctttt gccgatcacc
    16021 cacatattgc tggtcgttgg ataccccgcc agaccattgg caccaccctg tgcaaattgt
    16081 gcagcaatag tttcccggtt cttccctgct gttaacgcca gcagtgcgcc atccgcccct
    16141 tttgctggtc ggtcaagcat tggtgcaggt aaatcgtagc gtggcaacag agctgctgtt
    16201 tgcgagtttt gctgattaaa tttaagtggg tagttactct cttgtacggc aatagtggtt
    16261 ggcgctgatg ggtttaccag ccatttcaac tgattaaata ccgccatgcc ttgtgataca
    16321 ccatatttat cttttaaagc cgttagcagt gccaaattat caatttcgct agtgctatca
    16381 gagaagcggt ttgccatggt gcccacaaat gtcatcgcga catcaaacgg ttcccagcgc
    16441 ttaggagtaa agccaaatgt attaaactgt tttggtaaga gcgtctctgg attggtattt
    16501 accttatcaa tccaggcatt cattccatca gcgtagcctt gcagaatgga catatcctct
    16561 ggggaaaggg cagcaatttg cgcccggata gcatccggcc agtagttacg acggatatct
    16621 ttatcaaatt tcacaaaatc tttgccaagc acttccgcga cagtcccttg agtactgcga
    16681 cgtgccattt ccatctgaaa aaggcgatct tgtgctacta catagccata gccataaaat
    16741 aggtgccatg tatcattggc ataaatatgc ggcatgccgt attcatcgcg aacaatcttt
    16801 atctcacttg acgactgctc agccagtgca ggtaagctcc aataatacat cagggaagca
    16861 gtaacacagt tcacgatcat acgatttcta tttttcattg tatcctctgg caggtgtata
    16921 attagcgaat tgatactagc aacgaagctt cggttatcta tttgcagaga attgtttcat
    16981 tatgaactgt gagcgcgata actaattttt gatacaaaaa tttcattaat gtgatctaaa
    17041 acgaatcgct ttattcaccg ctgatgattt tgttctattc ctttttgtga cagatccccc
    17101 ggacgcatag atgatgaccc ccagagagac agctcaggtc attatctgta tgtaaatcag
    17161 aaaaatatgt tgtaaacgac ttttatggtt ggattttgta gcaattttga atgcagacag
    17221 gcagcatttg ccaacacgcc gcccatagtt cactccacat cctccctgcg taacccaata
    17281 tccctcaacc gttcatcact catctgctgt aacactcgtc gggtctgcat ttgccgccgc
    17341 caacgcctca ccgcatgcca gagttgcaca agtctgataa acggcgtttt ggctttgttt
    17401 tcgtgaaatt ccatctgctg cctcctcatc tttcagaggc ttcatcatcc gccttctggc
    17461 agttcacaat acagatgcaa atattagttt tatttaacat acagacaggt taaaacagca
    17521 tctgcatccg gttttttagc ctcatctgta ccggtttttc tttctgtatg ggccaaataa
    17581 ggaatacagc atgacgcgtt atcaacatct ggcgactcta cttgccgagc ggattgagca
    17641 agggctgtat cgtcacgggg agaaattgcc gtcggtgcgc agcttaagtc aggagcacgg
    17701 cgtcagcatc agcaccgtgc aacaggcgta tcagacgctg gaaacgatga ggctcatcac
    17761 tccgcagccg cgttcgggtt attttgtcgc acaacgtaaa gcccagccgc ctgtaccgcc
    17821 gatgacgcgt ccggtgcagc gcccggtgga aattacccag tgggatcagg tgctggatat
    17881 gctggaggcg catagcgaca attccattgt tccgttaagc aaaagcacgc cggatgtcga
    17941 aacgcccagc ctgaaaccgc tgtggcgtga gctaagccgg gtggtgcagc ataatctgca
    18001 aaccgttctc ggttatgact tgttagccgg tcagcgagtg ttgcgggagc agattgcccg
    18061 cctgatgctc gacagcggct cggtggtcac cgccgatgac atcatcatca ccagcggctg
    18121 ccataactcg atgtcgctgg cgttaatggc ggtgtgtaaa ccgggcgata ttgtcgcggt
    18181 cgaatctccc tgttattacg gttcgatgca gatgctgcga ggcatgggcg tgaaagtgat
    18241 tgaaatccca accgatccag aaactggcat cagcgttgaa gcactggaac tggcgctgga
    18301 acagtggccg attaaaggca tcattctggt gccaaactgt aataatccgc tgggatttat
    18361 tatgccggac gcacgcaaac gggccgttct ctctctcgct cagcgtcatg atattgtgat
    18421 ttttgaagat gatgtctacg gcgaactggc aacggagtat ccgcgcccgc ggaccatcca
    18481 ttcatgggat atcgacgggc gagtgttgtt gtgcagctcg ttcagtaaaa gtattgctcc
    18541 aggcctgcgc gtgggttggg tcgcaccggg gcgttatcac gataaactga tgcatatgaa
    18601 atacgccatc agcagcttta atgtgccgtc cacgcaaatg gcggcggcaa cgtttgtgct
    18661 ggaaggtcac tatcatcgcc atatccggcg gatgcggcag agctatcagc gcaatttggc
    18721 gctttatacc tgctggatac gggaatattt tccctgcgaa atctgtatta cgcgcccgaa
    18781 aggcggattt ttactgtgga tagaattgcc tgaacaggtc gatatggtct gcgttgcgcg
    18841 gcagctgtgc cgcatgaata tccaggtggc agcaggctcg attttctcgg cttccggcaa
    18901 ataccgtaat tgtctacgca tcaactgcgc tttgccgctc agcgaaacct atcgcgaagc
    18961 actaaagcaa attggcgagg ccgtgtatcg ggcaatggaa taaattcgaa ttagtggcga
    19021 agcacctgat ttcctctcac gaatcgcctg taactcgttg tatctttgca gggtcatcgc
    19081 cacgttccag catggcttag gcaaagtgtg cttcgttcat accggatgcg gcgtgaacgc
    19141 cttatccggc ctacaaaccg cgcaaattca atagattgca gaggaaatgt aggtctgata
    19201 agcgtagcgc atcaggcagc tttgcgtttg tcatccgtct ctgattagta ttcgatccat
    19261 ggatcgcgct tgatttcagg caagggctgc tctaatgcct gcatttgctc tgtcgaaagt
    19321 ccggtaatct tttgtatcca ttcaggatct gccccattct gcaacaacaa tctaaggagg
    19381 cgttgttccc cttcttcttt ccccttttcc atccccaaca gccatccatc attataaatt
    19441 cgctctgcaa tcgtcattat tcgctccctg tgttgtggca ttcgacgggt aagttcgctg
    19501 ataaacttct taaaacgcgc ttcatcgcca gtcagtaaaa tgtaatttaa cagcgcagtt
    19561 atctggctgt cattagcaca ctctgtaacc agtaatatta ccagttgatc gataagcccc
    19621 atcagatcgc gctggcgaat atgcttttgg atcaactcca acagggcgac tctgcggtgc
    19681 tgcacaatct cgtcgtctgg cacgacagtg acatccacca gcgggaacgc tgcggtataa
    19741 agcttccgtg cggtggtcgg gtcagcaaat tcgtccagcc agcacaggga ccagggataa
    19801 ggactacggc taccgtgata aaacagcatc gggatgacca gcggtagcgg ccggcgttta
    19861 tcatgctcga tatggcgctg catcaccgcc atggaatatc gcatcaggcg aaaggccata
    19921 tggatatcct cgcggctctg atgttcaatc actacgtaaa tataaccatc accttcacgg
    19981 gtctttaccg accacagaat atcggagtgt agcgcccgca atttttcatc gacgaagctg
    20041 gcggattcca gttttaagct gtcgagatcg cacagttcac gtaaatcttt gggtaagtgg
    20101 atctccataa aatcccgcgc ggtgtcaggg tgcgtgagaa aggatttaaa taatgcgtca
    20161 tgcggcgtgc tggttgtgaa gtttgtcatg gcgatccgtc acctgtagat aagatggccc
    20221 gactgtagcg actaacaaaa ccattctcac ccggcaattt ctcgttcccc gaggcgcttt
    20281 ccaggctatt tgtgttcctc tgcaaacgat acatttttcc ggaaacaggc tcgcgtttta
    20341 catttcctgt acccggcaga tattcccctt tccaccgcgc tcttttgatt aaccgcaaat
    20401 tttaattaca cttaaggtgt atattttcta tgcaacccat caattaaaga ggtgtaatgt
    20461 gctgatgact atttgtaatc gttatacatt ctgacctgga gtcagaaggt atttctctgt
    20521 ctgtgtgttc acaggcagtg tggttgatta catgaaattc agtacatttg tccttctcgc
    20581 ctgcctttcg tcattaccga cggtattgaa tttcgttttc cccgttgggg ttctccggac
    20641 aaggagttgt ttgttatgcc acgttttttt gcccgccatg ccgctacgct gtttttcccg
    20701 atggcgttga ttttgtatga ctttgccgcc tatctttcga cggatctgat ccagccgggg
    20761 atcattaatg tggtccgtga ttttaatgcc gatgtcagtc tggctccggc tgccgtcagt
    20821 ctctatctcg cggggggtat ggcgttacag tggctgctgg ggccgctttc cgacaggatt
    20881 ggccgcaggc cggtgctgat taccggggcg ctaatattta cccttgcctg cgctgcgaca
    20941 atgttcacaa cgtctatgac acagtttctt atcgcgcgtg caattcaggg caccagtatc
    21001 tgttttattg ctaccgttgg ttatgtcacg gtgcaggagg cgttcggaca gacaaaaggg
    21061 attaagttga tggcgattat cacctccatc gtactgattg cgccgattat tggcccgctt
    21121 tccggcgcag ctctgatgca ctttgtgcac tggaaagtcc tttttgccat cattgcggtt
    21181 atggggttta tctcatttgt tggcttactg ctggcgatgc cagagacggt gaagcgcggc
    21241 gcggttccat ttagcgccaa aagcgtcttg cgcgattttc gtaatgtctt ttgcaaccgg
    21301 ctgttcctct ttggcgcagc aaccatctct ttaagctata tcccgatgat gagttgggtg
    21361 gctgtctcgc cggtgatcct gattgacgca ggcggcttaa caacttcgca gttcgcctgg
    21421 acacaggttc cggtgttcgg cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt
    21481 aaagatccga ccgaaccgcg ctttatctgg cgtgctgtac ccattcaact ggtcggcctc
    21541 gcgctgttga ttatcggcaa tctgctgtca ccgcacgtct ggctgtggtc ggtgctgggc
    21601 accagtttgt atgctttcgg gattggtttg atcttcccga ccttattccg ctttacgctt
    21661 ttttccaata acttaccgaa aggaaccgtc tccgcatcgc tgaatatggt gatcctgatg
    21721 gtgatgtctg tctcggtcga aatcggccgc tggctgtggt ttaacggcgg tcgcttgccg
    21781 tttcatctgt tagccgtcgt ggcgggcgtt atcgtcgttt tcaccctggc aggattgctc
    21841 aatcgcgtgc gccaacatca ggctgccgag ctggcggagg agcggtgatt tttgcgcgat
    21901 cctgccgtca ggctctattc ttaacgttat gaataaactc attgaactca gacgcgccaa
    21961 aatgttggcg ctctctttac tgcttatcgc cgctgctacc tttgtcgtta cgctgttttt
    22021 gccgcccaat ttttgggtga gcggcgtgaa ggcgattgct gaagcggcga tggtcggcgc
    22081 gctggcggac tggtttgcgg tggtggcgct gtttcgccgc gtgccgattc cgatcatttc
    22141 tcgtcatacg gcgattatcc cgcgtaataa agaccggatt ggcgaaaatc tcggccagtt
    22201 cgtgcaggaa aaatttctcg atacccagtc gctggtggca ttgattcgac gtcacgaacc
    22261 ggcgttgttg attggcaact ggtttagtca gccggagaac gcccgccgcg ttggtcagca
    22321 tctgttgcag atcatgagcg gttttcttga actgaccgat gatgcgcgta ttcagcgcct
    22381 gcttaagcgc gcggtccatc gggcgattga taaggtcgat ctttccggca ccagtgcgtt
    22441 gatgctggag agtatgacca aaaacgatcg tcatcaggtg ctgctggata cgctgatcgc
    22501 gcagttgatt gctcttctcc agcgcgataa atcgcgcaag tttattgccc agcaaattgt
    22561 tcgctggctg gagagcgagc atccgctgaa agccaaaatt ttgcctactg aatggctggg
    22621 cgaacatagc gcggagttgg tttctgacgc ggtgaattct ttgcttgatg atatcagccg
    22681 tgatcgtgcg catcagatcc gttatgcgtt tgatcgcgcc acttttgccc tgatcgacaa
    22741 gttgaaaaac gatccggaaa tggcagcgcg agccgatgcc gtaaaaagtt atctgaaaga
    22801 agatgaagct tttaaccgct atctcagtga attgtggggg gatttacggg agtggctgaa
    22861 agcggatatc aacagtgaag attctcgtgt gaaagaacgt atcgcgcggg cgggtcaatg
    22921 gtttggcgaa acgttaattg ccgatgatgc cttgcgggcg tcgttaaatg gtcacctgga
    22981 acaagccgcg caccgcgtcg cgcctgagtt ttccgcattc ctgacgcgcc acatcagcga
    23041 tacagtaaaa agctgggatg cgcgggatat gtcacggcaa atcgagttaa atatcggcaa
    23101 agatctgcag tttatccgtg tcaacggtac gctggttggc ggttgtattg ggctaatttt
    23161 atatttgttg tcgcagctcc cggccttgtt ccccctcggc aatttttaga aatccatgaa
    23221 aaactaagtg gctaaccgcg agagagatca aataaaccac actaaaagaa tggtaataat
    23281 gtggcctctt ttcattatct cccgtggtac ggggaaggaa aatcatgtca cttatcaccg
    23341 atctacccgc cattttcgat cagttctctg aagctcgcca gaaaggcttt ctcaccgtca
    23401 tggatctcaa ggagcgcggc attccgctgg ttggcactta ctgcaccttt atgccgcaag
    23461 agatcccgat ggcagccggt gcggttgtgg tttcgctctg ttccacctct gatgaaacca
    23521 ttgaagaagc ggagaaagat ctgccgcgca acctctgccc gctgattaaa agtagctacg
    23581 gcttcggcaa aaccgataaa tgcccctact tctacttttc ggatctggtg gtcggtgaaa
    23641 ccacctgcga cggcaaaaag aaaatgtatg aatacatggc ggagtttaag cctgttcatg
    23701 tgatgcagct gccgaacagc gttaaggacg atgcctcgcg tgcattatgg aaagccgaga
    23761 tgctgcgctt gcaaaaaacg gtagaagaac gttttgggca cgagattagc gaagatgctc
    23821 tgcgcgatgc cattgcgctg aaaaaccgcg aacgtcgcgc actggctaat ttttatcatc
    23881 ttgggcagtt aaatcctccg gcgcttagcg gcagcgacat tctgaaagtg gtttacggcg
    23941 caaccttccg gttcgataaa gaggcgttga tcaatgaact ggacgcgatg accgcccgcg
    24001 ttcgtcagca gtgggaagaa ggccagcgac ttgacccgcg tccacgcatt ttaatcaccg
    24061 gctgcccgat tggcggcgca gcagaaaaag tggtgcgggc gattgaagag aatggcggct
    24121 gggttgtcgg ttatgaaaac tgcaccgggg cgaaagcgac cgagcaatgc gtggcagaaa
    24181 cgggcgatgt ctacgacgcg ctggcggata aatatctggc gattggctgc tcctgtgttt
    24241 cgccgaacga tcagcgcctg caaatgctca gccagatggt ggaagaatat caggtcgatg
    24301 gcgtagttga tgtgattttg caggcgtgcc atacctacgc ggtggaatcg ctggcgatta
    24361 aacgtcatgt gcgtcagcag cacaacattc cttatatcgc tattgaaaca gactactcca
    24421 cctcagatgt cgggcagctc agtacccgtg tcgcggcctt tattgagatg ctgtaaggag
    24481 tggcagtgac atattcgatt ggcattgatt ctggttctac cgccaccaaa gggatcttac
    24541 tggcagacgg cgtgattacg cgccgtttcc tcgttccaac accctttcgt ccggcaacag
    24601 caattactga agcctgggaa actctgcgcg aagggttaga gacaacgccg tttctgacgc
    24661 tcaccggcta tggacggcaa ctggtggatt ttgccgataa acaagtgacg gaaatctcct
    24721 gtcacgggct gggcgcacgg tttcttgcgc cagcaacgcg cgcggtaatc gacatcggtg
    24781 gtcaggacag caaagtgatt cagcttgatg atgacggtaa cctgtgcgat ttcctgatga
    24841 atgacaaatg cgcggcgggc accgggcgtt tcctggaggt gatctcgcgc acgcttggca
    24901 ccagcgtcga gcaactcgac agcattaccg aaaatgtcac gccgcatgcc atcacgagta
    24961 tgtgcacagt gtttgccgaa tcagaagtga tcagcctgcg ctcagcgggc gtcgcgccag
    25021 aagcgattct cgccggagtg attaacgcga tggcgcggag gagtgccaat ttcattgctc
    25081 gtctctcctg tgaagcgccg attctgttta ctggtggcgt tagtcattgc cagacgtttg
    25141 cccggatgct ggaatctcac ctgggaatgc cggtaaatac ccaccctgat gcgcaatttg
    25201 ctggcgcaat tggcgcggcg gtaattggtc aacgagtgag gacacgccga tgaaagagtt
    25261 tttattttta tttcactcca cagtcggcgt catacaaacc cgcaaagcgt tgcaggcagc
    25321 gggcatgact tttcgcgtca gcgatattcc ccgtgattta cgcggcggct gcgggttatg
    25381 catttggctg acctgtcccc ccggcgagga aatacaatgg gtgatccctg ggctgactga
    25441 gtcaatttat tgtcagcagg atggtgtctg gcgctgcatt gcacattacc gggtttctcc
    25501 gcgttaagga ttcattaagg tcgctacgta tacttctcga tatcttattg atttgcctta
    25561 aagggattac cgtgacaaaa agtatttcgt taagtaaacg catctccgtt attgtgattc
    25621 tttttgccat tgtggcagtg tgtactttct tcgtgcaatc ttgcgcccgt aaaagcaatc
    25681 atgcagcatc atttcagaat taccacgcca ccattgacgg caaagaaatt gcaggcataa
    25741 cgaataatat ctcttcgctg acctggtcgg cacaaagcaa tactcttttt agcactatca
    25801 acaaacctgc cgccattgtt gaaatgacca ccaatggtga ttttatccgc acaattcctc
    25861 tggattttgt taaggatctc gagacgatcg aatacattgg cgataatcag tttgtcatta
    25921 gcgatgagcg cgattacgcc atttatgtga tttcactgac cccaaactcg gaagtaaaaa
    25981 tcctcaaaaa aataaaaatc ccgctgcaag actccccaac caattgcggt tttgaagggt
    26041 tggcttattc tcgccaggat cacactttct ggttttttaa agagaaaaat ccgattgagg
    26101 tctacaaagt taacgggctg ttaagtagca atgaattaca catcagcaaa gacgaggcac
    26161 tacaacggca atttacttta gatgatgtgt ccggcgcaga atttaaccag caaaaaaata
    26221 cgcttctggt gctgtctcat gaatctcgcg cactccagga agtaaccctg gttggcgagg
    26281 ttatcggtga aatatcacta accaaaggta gtcgaggttt gtcgcataac atcaaacagg
    26341 ccgaagggat tgcgatggat gcctctggta atatttatat cgttagcgaa ccaaatcgtt
    26401 tttatcgctt tacccctaaa tcatcgcatt aacaggctgg catatttcac ctttttgtta
    26461 ttaacttgtt aacaacaaaa atgacataca aggttaaaca tgccttcgtc ctcgcatccc
    26521 gtagaacgct tttctttcag caccgcgctt ttcgggatgc tggttctgac cctaggtatg
    26581 ggattaggtc gcttccttta tacgcctatg ttgcccgtca tgatggcgga aggggcgttt
    26641 tcatttagcc agctctcgtg gattgccagc ggcaactatg cggggtatct ggctggcagt
    26701 ctgctatttt cgtttggcgc atttcaccag ccgtcgcgcc tacgcccatt tctgttggtt
    26761 tctgccctgg cgagcgggtt gttgatcctc gcaatggcat ggttgccgcc atttattctg
    26821 gtgttattga ttcgcttcct ggcgggtgtc gccagcgccg gtatgctgat ttttggttcg
    26881 acgctgatta tgcagcacac gcgccatcct tttgtgctgg cagctttgtt ttctggcgtt
    26941 ggcattggca ttgcactggg caacgaatat gttctggcag gcctgcattt tgccttatcc
    27001 tcgcaaacgt tatggcaagg cgccggggcg ctttctggca tgatgctgat tgcacttacg
    27061 cttttaatgc cctcgaaaaa acacgccatc gcaccaatgc cattggcaaa aacggagcaa
    27121 cagataatga gctggtggtt actggctatt ctgtacggcc ttgcgggatt tggttatatc
    27181 atcgtcgcga cttacctgcc gctcatggcg aaagatgcag gctcaccatt gttaaccgcc
    27241 catctctgga cgttagtcgg cttatcaatt gtgccaggct gctttggctg gctatgggca
    27301 gcaaaacgtt ggggggcttt gccttgcctg acggcgaatt tgctggtgca ggctatcagt
    27361 gtgctgctta ctctcgccag cgactctcct ctcttgctta tcattagtag tatcggtttt
    27421 ggcggtacct ttatgggcac gacatctctg gtgatgacta tcgcccgcca actgagcgtt
    27481 ccgggaaatc tcaatctttt aggctttgtg acgctcattt acggcattgg acaaatcctc
    27541 ggcccggcgc taaccagtat gctcggcaat ggaacatcgg cgcttgccgg cgccactctc
    27601 tgcggcgcgg cggcgttgtt tattgctgcg cttatcagca cagtacaatt atttaagctg
    27661 caagtggtca cttcatgagg agtgtttccc ttaccactca agaaactcaa cagcaaccgt
    27721 tgatgtcagc cagaccccgt tttccgcttg ccagaaaggg atgcctcgtt tcgccatttc
    27781 ctgtgctttg acggttaaga taaccggcga tccgtgtctt gctcccactt tgcgtgccgt
    27841 tgcttcatca gcagagagat gtacgtaatg acgctcccct gcaatcagcc cctgtttttt
    27901 tatctcgtca aggaaacggc ttgccgtacc gtggtagaga aactgtggcg gtgttttttc
    27961 ggcaaaggaa atggcgacct gcgaagtcga atgcccctga accgcgcgga tgcacctacc
    28021 atcactggaa taactaaaac gctttttatc gctggtggca accacagtat ccagcaatgc
    28081 acgggttaac cgcttgcccg ctttttgggc acaaagtatg agcttatcga tatccgccca
    28141 tccttcacgg tcaagtacga taccaatcgc ctccggtttg tgtcggagga caaaacttaa
    28201 aaacttgctc gtatccgcca actctttttc gttgtacttt gccatcgcgt tttcgtccct
    28261 ggattttctg tttttattta ctgatgtcat tgtcactggg tttatgaatt ttctgcaact
    28321 caatcctgcg cagagccttt tctacacagc gtaatttttc cgctatgcaa aaaacgcata
    28381 gcaagaaaca tacttgcatt ttccccatac ccacatggct gataaggttt ttcagctcca
    28441 ggaattttcc tggctcaagc aacacacaaa cacaaaaaaa caatggggat tgttatgaca
    28501 caacaagggg atgctgtagc cggggagctg gcgacagaaa aagtgggcat taaaggatat
    28561 ctggcttttt ttctcaccat aatctttttt tccggagttt tttccgggac agacagctgg
    28621 tggcgcgttt ttgatttttc cgttctgaac ggatcgtttg ggcaactgcc tggtgcgaat
    28681 ggagccacca cctcatttcg cggggcgggt ggcgcggggg ctaaagatgg ctttctcttt
    28741 gcgctggagc tggcaccgtc agtcattctc tctctgggga ttatttcaat taccgatggt
    28801 cttggtggat tacgcgccgc gcagcagtta atgacgcctg tgctaaaacc gttgttgggc
    28861 attccaggga tctgctcgct ggcgctgatc gctaacctgc aaaacactga tgccgccgcc
    28921 ggtatgacca aagagctggc gcaggaaggg gaaattaccg aacgcgataa agtgattttc
    28981 gccgcatatc aaaccagcgg tagcgccatc atcaccaact acttctcttc cggcgtcgcg
    29041 gtgttcgcct ttctgggcac gtccgtaatc gtacctttag ccgtcatcct ggtgtttaaa
    29101 tttgtcggag ccaacatttt gcgcgtctgg cttaacttcg aagaacgtcg caacccaacg
    29161 caaggagcgc aagcatgaca actcaggtcc gcaaaaacgt catggatatg tttatcgatg
    29221 gcgcgcgccg tggctttact atcgccacga ccaacctgct gccaaacgtg gtgatggcgt
    29281 ttgtcatcat tcaggcgctg aaaattaccg gcctgctcga ctgggtgggg catatctgtg
    29341 aacccgtaat ggcgctgtgg ggattacccg gtgaagcggc aaccgtcctt ttagccgcat
    29401 taatgagtat gggcggcgca gtcggtgttg ccgccagtct ggcgaccgcc ggcgcattaa
    29461 ccggtcacga tgtcaccgtt ttactgcccg cgatgtattt aatgggcaac ccggtgcaaa
    29521 atgtcggtcg ctgcctgggg acagccgaag tgaatgccaa atattacccg catatcatta
    29581 cggtctgcgt gattaacgca ttgctgtcga tctgggttat gcagcttatt gtttaacaag
    29641 gagttaccat gattgattat accgcagccg gttttaccct gctgcaggga gcgcatttgt
    29701 atgcgccgga agatcgggga atttgcgatg tcctcgtcgc taacggcaaa attatcgccg
    29761 ttgccagcaa tatcccttct gacattgtac cggactgcac ggttgtcgat ctcagcgggc
    29821 agatcctctg tcctggtttt attgatcaac acgtccattt gattggtggt ggcggcgaag
    29881 caggtcccac gacgcgcacg ccagaagtgg cgctaagtcg cctgacggaa gcgggcgtca
    29941 cgtcagtggt tggtctgctg ggcaccgact ctatctctcg ccacccggaa tccctgctcg
    30001 ccaagacccg tgcgctcaat gaagaaggca tcagcgcctg gatgctgacc ggcgcttatc
    30061 atgtcccttc ccgcaccatt acaggttccg tggaaaaaga cgtggcgatt atcgatcgtg
    30121 tgattggcgt gaaatgcgcc atctctgatc accgttctgc cgcaccggac gtttatcacc
    30181 tggctaatat ggcggcggaa tcccgcgttg gcggtttgct cggcggtaaa cctggcgtca
    30241 ccgtgttcca catgggcgac agcaaaaagg cgttacagcc tgtctatgac ctgctggaaa
    30301 actgcgatgt gccgatcagc aagttgctgc cgacccacgt taaccgcaac gttccgttgt
    30361 ttgagcaggc gctggagttc gcgcgcaaag gcggcactat cgatatcacc agcagcattg
    30421 acgaaccggt cgcccctgcc gaaggtattg cccgcgccgt tcaggcgggt attccgctgg
    30481 cacgcgtcac cctcagctcc gacggcaacg gtagccagcc gttcttcgat gacgaaggga
    30541 atttaaccca tatcggtgtt gccggttttg aaacgttgct ggaaaccgtg caggtgctgg
    30601 tcaaagacta tgatttcagt atcagcgatg ccctgcgccc gctcaccagt agcgtagccg
    30661 gtttccttaa cctgaccggg aaaggcgaaa ttctgccagg caatgatgct gacttgctgg
    30721 tcatgacgcc agaactgcgc attgagcagg tatacgctcg cggcaaactg atggtcaaag
    30781 acggcaaagc ctgcgtgaat ggaacgtttg aaacggctta aacggttgtc agccgcccga
    30841 atggacgtag aatgcccgca agggcggctg acagagtaaa acgtaatgga tgactgtggt
    30901 gcgattttgc ataatattga aaccaaatgg ctttatgatt ttctgaccct ggaaaaatgc
    30961 cgcaattttt cccaggcggc agtcagtcgc aacgtctcgc aaccggcatt cagccgccgc
    31021 atccgtgcgc tggaacaggc gattggtgtt gaattgttta accgccaggt aacgccgctg
    31081 caactctcag aacaagggaa aatcttccat tcgcagatcc gccatctgtt gcaacagtta
    31141 gagagcaatc tggcagagct gcgtggcggc agcgattacg cgcaacgaaa aatcaagata
    31201 gccgctgcac actctctttc cctcgggctg ttaccgtcca ttatcagcca gatgccgccg
    31261 ctctttacct gggcaattga agctattgat gtcgatgaag cggtcgataa actgcgtgaa
    31321 gggcaaagtg actgtatttt ttcctttcac gacgaagatt tgctggaagc gccgtttgac
    31381 cacattcgct tatttgaatc tcaattgttc cctgtctgcg ccagtgacga acacggagaa
    31441 gcacttttta acctcgcgca gccacacttt ccgttactga attacagccg caactcctac
    31501 atggggcgat tgattaatcg caccctgacg cgccacagtg agttaagttt cagcaccttt
    31561 tttgtctctt cgatgagcga gcttttaaag caggttgccc tcgacggctg tgggattgcc
    31621 tggctgccgg agtacgccat ccaacaagaa attcgcagcg ggaagctcgt tgtactgaat
    31681 cgggacgaac tggtgatccc gattcaggcc tatgcatacc gaatgaatac ccgcatgaat
    31741 ccagttgccg aacgcttctg gcgtgaactg cgcgagctgg agattgtgct tagctgacat
    31801 tctccagcat cgcactacac caaccgccat tatcccagac cagatcaaac gttagcggtt
    31861 caccgtcgtg gtacagcatt atctcaaggc tgatcgccag agtgtggcag tacgcttctt
    31921 taaaattaac ctgcaacgcc gcaatccgtg gctcatgaaa taacagtgca gaacgcagct
    31981 gacggcaata ccagctctgg agatgtttgt cgctctggtt atgcacttgc cactctggca
    32041 tgcctgaaac aagtaatgac ggtaatgccg aatgaggaga attaaaaaga gtgaggagat
    32101 cgctgcatac cgatttacgc agcaatgacg ttttattgcc tgaaatttca ggtaaaacag
    32161 cctgaagtga ttggcgcatc attttgcgca ctcctgacgt ttagcaacat atgcggcttg
    32221 ccctgaacac cgggcaccac tgtattgatg ctcattttcc atagccattc ccttttgttc
    32281 attacatcca tgtcagtctg agacaggcct gaatcctgtc agcaaagtat aaaaccttac
    32341 ttgcctatga atatctactg acttttcagg aaaaaagtca tgat
//
LOCUS       DAZFRN010000043        32345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFRN010000043 DAZFRN010000000
VERSION     DAZFRN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.043"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(95..1855)
                     /gene="ycaO"
                     /locus_tag="ACHKUB_001932"
     CDS             complement(95..1855)
                     /gene="ycaO"
                     /locus_tag="ACHKUB_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO6654956.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1985..2677
                     /locus_tag="ACHKUB_001933"
     CDS             1985..2677
                     /locus_tag="ACHKUB_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO6654957.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2876..3964
                     /gene="serC"
                     /locus_tag="ACHKUB_001934"
     CDS             2876..3964
                     /gene="serC"
                     /locus_tag="ACHKUB_001934"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO6654958.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4035..5318
                     /gene="aroA"
                     /locus_tag="ACHKUB_001935"
     CDS             4035..5318
                     /gene="aroA"
                     /locus_tag="ACHKUB_001935"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO6654959.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5487..6251
                     /gene="ycaL"
                     /locus_tag="ACHKUB_001936"
     CDS             5487..6251
                     /gene="ycaL"
                     /locus_tag="ACHKUB_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO6654960.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6424..7107
                     /gene="cmk"
                     /locus_tag="ACHKUB_001937"
     CDS             6424..7107
                     /gene="cmk"
                     /locus_tag="ACHKUB_001937"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO6654961.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7218..8891
                     /gene="rpsA"
                     /locus_tag="ACHKUB_001938"
     CDS             7218..8891
                     /gene="rpsA"
                     /locus_tag="ACHKUB_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO6654962.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9051..9335
                     /gene="ihfB"
                     /locus_tag="ACHKUB_001939"
     CDS             9051..9335
                     /gene="ihfB"
                     /locus_tag="ACHKUB_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO6654963.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9542..11806
                     /locus_tag="ACHKUB_001940"
     CDS             9542..11806
                     /locus_tag="ACHKUB_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO6654964.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11843..13591
                     /gene="msbA"
                     /locus_tag="ACHKUB_001941"
     CDS             11843..13591
                     /gene="msbA"
                     /locus_tag="ACHKUB_001941"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO6654965.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13588..14574
                     /gene="lpxK"
                     /locus_tag="ACHKUB_001942"
     CDS             13588..14574
                     /gene="lpxK"
                     /locus_tag="ACHKUB_001942"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO6654966.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14611..15843
                     /gene="ycaQ"
                     /locus_tag="ACHKUB_001943"
     CDS             14611..15843
                     /gene="ycaQ"
                     /locus_tag="ACHKUB_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO6654967.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGTFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTKMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15895..16077
                     /gene="ycaR"
                     /locus_tag="ACHKUB_001944"
     CDS             15895..16077
                     /gene="ycaR"
                     /locus_tag="ACHKUB_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO6654968.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16074..16820
                     /gene="kdsB"
                     /locus_tag="ACHKUB_001945"
     CDS             16074..16820
                     /gene="kdsB"
                     /locus_tag="ACHKUB_001945"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO6654969.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16974..17867
                     /locus_tag="ACHKUB_001946"
     CDS             16974..17867
                     /locus_tag="ACHKUB_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO6654970.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17844..18623)
                     /gene="elyC"
                     /locus_tag="ACHKUB_001947"
     CDS             complement(17844..18623)
                     /gene="elyC"
                     /locus_tag="ACHKUB_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO6654971.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPHEQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18759..19544
                     /gene="cmoM"
                     /locus_tag="ACHKUB_001948"
     CDS             18759..19544
                     /gene="cmoM"
                     /locus_tag="ACHKUB_001948"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO6654972.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19541..20863
                     /gene="mukF"
                     /locus_tag="ACHKUB_001949"
     CDS             19541..20863
                     /gene="mukF"
                     /locus_tag="ACHKUB_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO6654973.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20844..21548
                     /gene="mukE"
                     /locus_tag="ACHKUB_001950"
     CDS             20844..21548
                     /gene="mukE"
                     /locus_tag="ACHKUB_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO6654974.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21548..26008
                     /gene="mukB"
                     /locus_tag="ACHKUB_001951"
     CDS             21548..26008
                     /gene="mukB"
                     /locus_tag="ACHKUB_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO6654975.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26269..28116
                     /gene="ldtD"
                     /locus_tag="ACHKUB_001952"
     CDS             26269..28116
                     /gene="ldtD"
                     /locus_tag="ACHKUB_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6654976.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28297..28845
                     /locus_tag="ACHKUB_001953"
     CDS             28297..28845
                     /locus_tag="ACHKUB_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO6654977.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28872..29519
                     /gene="gloC"
                     /locus_tag="ACHKUB_001954"
     CDS             28872..29519
                     /gene="gloC"
                     /locus_tag="ACHKUB_001954"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO6654978.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29741..30931)
                     /gene="aspC"
                     /locus_tag="ACHKUB_001955"
     CDS             complement(29741..30931)
                     /gene="aspC"
                     /locus_tag="ACHKUB_001955"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO6654979.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31116..32204)
                     /gene="ompF"
                     /locus_tag="ACHKUB_001956"
     CDS             complement(31116..32204)
                     /gene="ompF"
                     /locus_tag="ACHKUB_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO6654980.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7858 a         7866 c         8710 g         7911 t
ORIGIN      
        1 taatgcaaat atatgtaaag cggggcatta aaaaaacgcc ccgtaatata actcagacta
       61 atcattaagc ctacattgcg taggctattt gattttattt tgcccagaat gctgctttgg
      121 cgcgctgcag cttttcgtag gccttcaaca acgactgatg tgcagcaaac gcgtgcagat
      181 cgctatctac cggttgcagg ccgtaaaacg ccgcttcgcc gctcattgcc gcactggcgg
      241 cttctacggc atctgcgccg tacatgcgta caaacgcatt cagatattgc agcggctggc
      301 gatcttcttc ctgtgccagt aataacaacg tttgcaggca gcgataatag ttggcgcgtt
      361 ccggactaaa tactgatgag ttaaactcca tcgtccattc ggtccagacc agagcctgtt
      421 ccagatcgcc accagccagc gccagcatgg cttttaattc accgatacgc agggtgtacc
      481 aaccgttatc cgacccggtc gccagaccca acagctcacg cacgcgggta aagtcatcaa
      541 aaccttcttc atccagttgc tcgatgaggt tcaggtaatc ttctttttcc cactcgctgc
      601 ctggtagcga aagaatcgtt tcacgtaaat ggctgcccat actgttattc gcgagccaca
      661 gatcttcagc cggataaata tcggacatgc caggcacgat aatgcggcaa gcgtagacac
      721 ccagatgctc gtaatcggca atataaactt ctttatcttc ctttttgaag atagccatca
      781 gcgtggcgaa ctcttcttcc gtggtgccgg agaaattcca gtccacaaac ggataatcgg
      841 catcctgctt gaacaggtcc caggagatta aaccgctgga atcgataaag tgcgtttcga
      901 ggttggtatg ttcagcgact tcttcatcat cgaaggttgg cggagtaaac acatccaaat
      961 ctttcaggcc acgaccttgc agcagctcgg tcacggtacg ttccagtgct acgccaaaat
     1021 caggatgcgc accgaaagag gcaaagcagg taccgttagc aggattgaac agtaccacgc
     1081 aaatcaccgg atactggccg ccaagcgaac cgtcataagc gaagattggg aaaccctccg
     1141 cttccagcgt ttcgatggct tcaactactg ctgggtaacg cgccagcacg tctgccggga
     1201 tctctggcag gctgatgctt tcagcaataa tgcggttttt cacgtagcgt tcgaaaactt
     1261 cagacaatcc ctgaacgcgt gcttcgttgc gggtattacc tgcggacata ccgttggaga
     1321 cgtacaggtt accaatgata ttcatcggaa tataaacggt ctgattgtcg gactgacgcg
     1381 taaacggcag gccgcaaata ccacgatctt cgttaccgga ttgtaggtca atcagcatgc
     1441 tgccggtcag ttcattctcc ggatcataaa acgcgcgcag acggtcatcg agtagtcctt
     1501 ctggcacatc gtcattttcg gtcagtggga accatttttc gttgggataa tgcacgaacg
     1561 gaccgttggc gatggtttcg cccagccaga aatcagcaaa aaagtagttg gttgagagac
     1621 gctcgaaata ttcaccaagt gcagaagcca gcgccgcttt tttggttgcg cctttaccgt
     1681 tggtaaaaca cagtgcgcac tctttgtcgc gaatatgtac agaccagacg ttaggcacag
     1741 gattcagcca ggaggcctct tcaatctgaa agccgaggtc tgaaagtttt tgctggaagc
     1801 gagcgatgga atcttccaga gcggcatctt tgccggggat aaatgtttgc gtcatgaaaa
     1861 tcactttagt cgtacggaaa gcgcgcaata atacgggttt tatctcaaag gcgctatcac
     1921 cgccgccatg ccgatgaact gttgactatg cttttagcgg ataaaccacg ttaagagcat
     1981 aaaaatgaag gcgttcgatc tccaccgtat ggcatttgat aaagtgcctt ttgatttcct
     2041 tggcgaagtt gcactacgta gtctttatac ctttgtactg gtctttttgt tcctcaaaat
     2101 gaccggaaga cgcggtgtgc ggcagatgtc gctgtttgaa gttttaatca ttctgacgct
     2161 gggatcagcg gcgggagatg tggcgtttta tgatgatgtg ccgatggtcc cggtacttat
     2221 cgtctttatt actctggcgt tgttataccg cctggtaatg tggttgatgg cgcacagtga
     2281 aaaactggaa gatcttctgg aaggcaagcc agttgtcatt attgaagatg gcgagctggc
     2341 ctggtcgaaa ctcaataact ccaacatgac ggaatttgag ttctttatgg agctacgatt
     2401 gcgtggcgtg gagcagctgg ggcaggtacg tctggcgatt ctcgaaacca acgggcaaat
     2461 cagtgtctat ttctttgaag atgacaaggt gaaaccgggt ttacttattt tacccagtga
     2521 ttgtacgcag cgttacaaag tggtgccgga gtcggcggac tatgcctgta ttcgttgtag
     2581 tgaaatcatt catatgaaag cgggggaaaa acaattatgt ccgcgctgtg caaatccaga
     2641 atggacgaag gcaagtcggg caaaacgggt gacctgacag taaaaacatc ggctttttgc
     2701 taataatccg agagattctt ttgtgtgatg caagccacat ttttgccctc aacggtttta
     2761 ctcattgcga tgtgtgtcac tgaatgataa aaccgatagc cacaggaata atgtattacc
     2821 tgtggtcgca atcgattgac cgcgggttaa tagcaacgca acgtggtgag gggaaatggc
     2881 tcaaatcttc aattttagtt ctggtccggc aatgctaccg gcagaggtgc ttaaacaggc
     2941 tcaacaggaa ctgcgcgact ggaacggtct tggtacgtcg gtgatggaag tgagtcaccg
     3001 tggcaaagag ttcattcagg ttgcagagga agccgagaag gattttcgcg atcttcttaa
     3061 tgtcccctcc aactacaagg tattattctg ccatggcggt ggtcgcggtc agtttgctgc
     3121 ggtaccgctg aatattctcg gtgataaaac caccgcagat tatgttgatg ccggttactg
     3181 ggcggcaagt gccattaaag aagcgaaaaa atactgcacg cctaatgtct ttgacgccaa
     3241 agtgactgtt gatggtctgc gcgcggttaa gccaatgcgt gaatggcaac tctctgataa
     3301 tgctgcttat atgcattact gcccgaatga aaccatcgac ggtatcgcca tcgacgaaac
     3361 gccagacttc ggcaaagatg tggtggtcgc cgccgacttc tcttcaacca ttctttcccg
     3421 tccgattgac gtcagccgtt atggcgtgat ttacgctggc gcgcagaaaa atatcggccc
     3481 ggctggcctg acaatcgtca tcgttcgtga agatttgctg ggcaaagcga atatcgcgtg
     3541 tccgtcgatt ctggattatt ccatcctcaa cgataacggc tccatgttta acacgccgcc
     3601 gacatttgcc tggtatctat ctggtctggt ctttaaatgg ctgaaagcga acggcggtgt
     3661 agcagaaatg gataaaatca atcagcaaaa agcagaactg ctatatgggg tgattgataa
     3721 cagcgatttc taccgcaatg acgtggcgaa agctaaccgt tcgcggatga acgtgccgtt
     3781 ccagttggcg gacagtgcgc ttgacaaatt gttccttgaa gagtcttttg ctgctggcct
     3841 tcatgcactg aaaggtcacc gtgtggtcgg cggaatgcgc gcttctattt ataacgccat
     3901 gccgctggaa ggcgttaaag cgctgacaga cttcatggtt gagttcgaac gccgtcacgg
     3961 ttaatgccga aattttgctt aatccccaca gccagcctgt ggggttttta tttctgttgt
     4021 agagagttga gttcatggaa tccctgacgt tacaacccat cgctcgtgtc gatggcacta
     4081 ttaatctgcc cggttccaag agcgtttcta accgcgcttt attgctggcg gcattagcac
     4141 acggcaaaac agtattaacc aatctgctgg atagcgatga cgtgcgccat atgctgaatg
     4201 cattaacagc gttaggggta agctatacgc tttcagccga tcgtacgcgt tgcgaaatta
     4261 tcggtaacgg cggtccatta cacgcagaag gtgccctgga gttattcctc ggtaacgccg
     4321 gaacggcaat gcgtccgctg gcggcagctc tttgtctggg tagcaatgat attgtgctga
     4381 ccggtgagcc gcgtatgaaa gaacgcccga ttggtcatct ggtggatgcg ctgcgcctgg
     4441 gcggggcgaa gatcacttac ctggaacaag aaaattatcc gccgttgcgt ttacagggcg
     4501 gctttactgg cggcaacgtt gacgttgatg gctccgtttc cagccaattc ctcaccgcac
     4561 tgttaatgac tgcgcctctt gcgccggaag atacggtgat tcgtattaaa ggcgatctgg
     4621 tttctaaacc ttatatcgac atcacactca atctgatgaa gacgtttggt gttgaaattg
     4681 aaaatcagca ctatcaacaa tttgtcgtaa aaggcgggca gtcttatcag tctccgggta
     4741 cttatttggt cgaaggcgat gcatcttcgg cttcttactt tctggcagca gcagcaatca
     4801 aaggcggcac tgtaaaagtg accggtattg gacgtaacag tatgcagggt gatattcgct
     4861 ttgctgatgt gctggaaaaa atgggcgcga ccatttgctg gggcgatgat tatatttcct
     4921 gcacgcgtgg tgaactgaac gctattgata tggatatgaa ccatattcct gatgcggcga
     4981 tgaccattgc cacggcggcg ttatttgcaa aaggcaccac cacgctgcgc aatatctata
     5041 actggcgtgt taaagagacc gatcgcctgt ttgcgatggc aacagaactg cgtaaagtcg
     5101 gcgcggaagt ggaagagggg cacgattaca ttcgtatcac tcctccggaa aaactgaact
     5161 ttgccgagat cgcgacatac aatgatcacc ggatggcgat gtgtttctcg ctggtggcgt
     5221 tgtcagatac accagtgacg attcttgatc ccaaatgcac ggccaaaaca tttccggatt
     5281 atttcgagca gttggcgcgg attagccagg cagcctgaat gaacaacggg caataaatag
     5341 ccaaatcttt ctttatcaaa acgtcggcac attgtcggcg ttttttttcg gaccttgtga
     5401 gtcattttga ttaatggtag cgtcgcttgt caatgtaagt tgttgataca taatatttat
     5461 atatgattaa tcaacggatg attcacatga agaatactaa attactgctg gcgattgcga
     5521 cctctgcagc attactgaca gggtgtcaaa atacccacgg tattgatacc aatatggcta
     5581 tcagctccgg tttaaatgcc tataaagcag caacattaag cgatgccgat gcaaaagcga
     5641 ttgccaatca gggctgtgcc gaaatggaca gcggcaatca agtcgcaagt aaatccagca
     5701 agtacggtaa acgtctggca aaaatcgcca aagcattggg taacaatatt aatggcacgc
     5761 cggtcaacta taaggtttat atgaccagcg acgtcaacgc atgggcgatg gcgaacggct
     5821 gtgtccgtgt ctacagtggc ctgatggaca tgatgaatga caacgaaatt gaaggtgttc
     5881 tgggccatga actgggccac gtcgcgttgg gtcactcgct ggctgaaatg aaagcttctt
     5941 atgcgatcgt tgccgcacgc gatgccattt cagctaccag cggtgtggct tcccagcttt
     6001 cccgctcaca attgggtgat atcgcagaag gcgctattaa tgctaaatac tcccgtgata
     6061 aagagtccga agcagatgat ttctcctttg atctgttgaa gaaacgtggc atcagcaccc
     6121 aggggctggt tggcagcttt gaaaaactgg ctagtctgga tggcggtcgc acccagtcca
     6181 tgtttgactc tcacccacca tcaacagagc gtgcgcaaca catccgtgat cgtatcgcct
     6241 ctggtaagta aatcattgtc atctttcggg ctggtcttct gccagcccgc tataattgcg
     6301 caataaatcc ccatctgaat acagacaaaa ctggtttttg cacacaacgt taacgatttg
     6361 tggcgtcggc gcgtataatg cgcgcggtta tgttaacggt acgcctgttt taaggagata
     6421 aagatgacgg caattgcccc ggttattacc attgatggcc caagcggtgc agggaaaggc
     6481 accttgtgta aggctatggc ggaagcattg caatggcatc tgctggattc gggtgcaatt
     6541 tatcgcgtac tggcattggc ggcattacat caccatgttg atgttgcgtc agaagatgcg
     6601 ctggtaccgc tggcatccca tctggatgta cgttttgtgt caaccaatgg caatctggaa
     6661 gtgatcctcg aaggggaaga tgtcagcggc gaaattcgta ctcaggaagt ggcgaatgca
     6721 gcttcacaag tcgcggcatt cccacgcgtt cgtgaagcat tattgcgtcg ccaacgcgcg
     6781 tttcgcgaat tacccggcct gattgccgat ggccgcgaca tgggaacggt ggtattccct
     6841 gatgcgccag tgaaaatttt ccttgacgcc tcctcggaag aacgtgcgca tcgccgcatg
     6901 ctacagttgc aggagaaggg ctttagtgtt aactttgagc gccttttggc cgagatcaaa
     6961 gaacgcgacg accgcgatcg taaccgagcg gtagcgccac tggttccggc agccgatgct
     7021 ttagtgttgg attccaccac cttaagcatt gagcaagtga ttgaaaaagc gctacaatac
     7081 gcgcgccaga aattggctct cgcataagcg accgaatttg cagtaccccc gttgcaatgg
     7141 aatgacagcg ggtatgttaa acaaccccat ccggcatgga gccaggtgga cgttaaatat
     7201 aaacctgaag attaaacatg actgaatctt ttgctcaact ctttgaagag tccttaaaag
     7261 aaatcgaaac ccgcccgggt tctatcgttc gtggcgttgt tgttgctatc gacaaagacg
     7321 tagtactggt tgacgctggt ctgaaatctg agtccgccat cccggctgag cagttcaaaa
     7381 acgcccaggg cgagctggaa atccaggtag gtgacgaagt tgacgttgct ctggacgcag
     7441 tagaagacgg cttcggtgaa actctgctgt cccgtgagaa agctaaacgt cacgaagcct
     7501 ggatcacgct ggaaaaagct tacgaagatg ctgaaactgt taccggtgtt atcaacggca
     7561 aagttaaggg cggcttcact gttgagctga acggtattcg tgcgttcctg ccaggttctc
     7621 tggtagacgt tcgtccggtg cgtgacactc tgcacctgga aggcaaagag cttgaattca
     7681 aagtaatcaa gctggatcag aagcgcaaca acgttgttgt ttctcgtcgt gccgttatcg
     7741 aatccgaaaa cagcgcagag cgcgatcagc tgctggaaaa cctgcaggaa ggcatggaag
     7801 ttaaaggtat cgttaagaac ctcactgact acggtgcatt cgttgatctg ggcggcgttg
     7861 acggcctgct gcacatcact gacatggcct ggaaacgcgt taagcatccg agcgaaatcg
     7921 tcaacgtggg cgacgaaatc actgttaaag tgctgaagtt cgaccgcgaa cgtacccgtg
     7981 tatctctggg cctgaaacag ctgggcgaag atccgtgggt agctatcgct aaacgttatc
     8041 cggaaggtac caaactgact ggtcgcgtga ccaacctgac cgactacggc tgcttcgttg
     8101 aaatcgaaga aggcgttgaa ggcctggtac acgtttccga aatggattgg accaacaaaa
     8161 acatccaccc gtccaaagtt gttaacgttg gcgatgtagt ggaagttatg gttctggata
     8221 tcgacgaaga acgtcgtcgt atctccctgg gtctgaaaca gtgcaaagct aacccgtggc
     8281 agcagttcgc ggaaacccac aacaagggcg accgtgttga aggtaaaatc aagtctatca
     8341 ctgacttcgg tatcttcatc ggcctggacg gcggcatcga cggcctggtt cacctgtctg
     8401 acatctcctg gaacgttgca ggcgaagaag cagttcgtga atacaaaaaa ggcgacgaaa
     8461 tcgctgcagt tgttctgcag gttgacgcag aacgtgaacg tatctccctg ggcgttaaac
     8521 agctcgcaga agatccgttc aacaactggg ttgctctgaa caagaaaggc gctatcgtaa
     8581 ccggtaaagt aactgcagtt gacgctaaag gcgcaaccgt agaactggct gacggcgttg
     8641 aaggttacct gcgtgcttct gaagcatccc gtgaccgcgt tgaagacgct accctggttc
     8701 tgagcgttgg cgacgaagtt gaagctaaat tcaccggcgt tgatcgtaaa aaccgcgcaa
     8761 tcagcctgtc tgttcgtgcg aaagacgaag ctgacgagaa agatgcaatc gcaactgtta
     8821 acaaacagga agatgcaaac ttctccaaca acgcaatggc tgaagctttc aaagcagcta
     8881 aaggcgagta attctctgac tcttcgggat ttttattccg aagtttgttg agtttacttg
     8941 acagattgca ggtttcgtcc tgtaatcaag cactaagggc ggctacggcc gcccttaatc
     9001 aatgcagcaa cagcagccgc ttaatttgcc tttaaggaac cggaggaatc atgaccaagt
     9061 cagaattgat agaaagactt gccacccagc aatcgcacat tcccgccaag acggttgaag
     9121 atgcagtaaa agagatgctg gagcatatgg cctcgactct tgcgcagggc gagcgtattg
     9181 aaatccgcgg tttcggcagt ttctctttgc actaccgcgc accacgtacc ggacgtaatc
     9241 cgaagactgg cgataaagta gaactggaag gaaaatacgt tcctcacttt aaacctggta
     9301 aagaactgcg cgatcgcgcc aatatttacg gttaagtttt tactcaaact tgaacgcgag
     9361 aaaagcacct gtcgggtgct tttttcattt ctctaatcag gaactggaag ctgcctcgca
     9421 gagttttgag cagttttcac cctttcgtta aattcttctg aatgtgcctc ggggaacgca
     9481 aaattcccac cagacaaccg ctcaacaaag ttgcacactt tccataaaca gggaggggtg
     9541 catgaaaata acgacagtcg gcgtatgcat aatttgcgga atttttccgt tgctgatttt
     9601 gccccaattg cctgggacat taacccttgc gtttctgact ctcttcgcct gcgtactggc
     9661 atttatccct gttaaaaccg tccgttatat cgcgctgacg ttgctgtttt tcgtttgggg
     9721 catattatca gcaaagcaaa ttttgtgggc aggagaaacc ttaactggcg cgacgcagga
     9781 tgcaattgtt gagatcactg caactgacgg catgaccact cattacggtc aaattactca
     9841 tctacaaggt cgacgtatat tccctgcgcc aggccttgtg ctgtatggcg aatatcttcc
     9901 gcaagcggtt tgtgctggac aacaatggtc aatgaaactc aaagttcgtg cggttcatgg
     9961 ccaacttaat gatggcggct ttgatagcca gcgttatgcc attgcccagc atcaaccgct
    10021 caccggccgc tttctgcagg caagtgttat tgaaccgaat tgtagcctgc gtgcacagta
    10081 tctggcgtca ctacaaacaa cgctgcaacc ctatatgtgg aatgcggtta ttcttggttt
    10141 aggtatgggg gaacggttat ccgtccccaa agaaatcaaa aatatcatgc gtgatactgg
    10201 aacggcgcat ttaatggcga tatcgggatt gcacatcgct tttgcggcgt tgctggctgc
    10261 cggactcatt cgcggtgggc aagtttatct gcctgggcgc tggatccact ggcaaatgcc
    10321 attaattggt ggaatctgct gtgctgcttt ttatgcctgg ttgacgggaa tgcaacctcc
    10381 tgcattgcgt accgtggtgg cgcttgctac gtggggaatg cttaagttaa gtgggcgaca
    10441 gtggagtggc tgggatgtat ggatatgttg tctggcggca attttgctga tggatcctgt
    10501 tgccattctc tcgcaaagtt tatggctctc tgccgctgcg gtcgcggcac tgattttttg
    10561 gtaccagtgg tttccctgtc ctgagtggca actgccgccg gtattgcgtg cagttgtttc
    10621 cctcattcat ttgcaactgg gaatcacact cctgcttatg cctgtgcaaa tcgtcatatt
    10681 tcatggcatt agtctgacct cgtttattgc aaatctatta gcaattccct tggtgacatt
    10741 tatcacggtt ccgttgatcc tcgccgcgat ggttgtgcat ttaagcgggc cgttaatcct
    10801 ggagcaaggg ttatggtttc ttgccgaccg gtctttggct ttacttttct gggggttaaa
    10861 gagtttgcca gaagggtgga tcaacattgc tgaacgttgg caatggctat cattttcccc
    10921 atggttctta ctggtggtat ggcgattaaa cgcctggcga acgttgccag caatgtgtgt
    10981 ggctgtaggc ttgctgatgt gctggccgct gtggcaaaaa cctcgacctg acgagtggca
    11041 ggtgtacatg cttgatgtcg ggcaagggct ggcaatggtg atagccagaa acggcaaagc
    11101 gattctctat gacacaggac tggcctggcc cgaaggggat agtgggcaac aactgattat
    11161 cccctggctc cactggcata atcttgaacc ggaaggcgtt attctgagtc atgaacatct
    11221 ggatcaccgg ggagggctgg actcaatatt gcacacatgg ccgatgttat ggatcagaag
    11281 tccgttaaac tgggaacatc atcagccctg tgtgcgtggc gaagcgtggc aatggcaagg
    11341 attgcgtttc agcgtgcact ggcctttaca agctagcaac gataaaggaa ataaccattc
    11401 ctgtgtggtt aaggttgatg acgggacgaa tagcattctt ctaaccggtg atattgaagt
    11461 ccccgctgaa caaaagatgc taagccgtta ctggcagcaa gtgcagacaa cattgcttca
    11521 ggtacctcac catggcagta atacctcatc atcgttgcca ttaattcagc gagtgaatgg
    11581 aaaagtggca ctcgcatcgg catcgcgcta taacgcatgg cgattgccct ctaataaagt
    11641 taagcatcgc tatcaacagc aaggatatca atggcttgat actccacatc agggtcaagt
    11701 gacggtcaat ttttcagcgc aaggctggcg gattagcagc ctcagagagc aaattttacc
    11761 tcgttggtat catcagtggt ttggcgtgcc agtggataac gggtagaata tgcggctatt
    11821 tcaacaaatg ctggtttttt gaatgcataa cgacaaagat ctctctacgt ggcagacatt
    11881 ccgccgactg tggccaacca ttgcgccttt taaagcgggt ctgatcgtgg cgggcgtagc
    11941 gttaatcctc aacgcagcca gcgatacctt catgttatcg ctccttaagc cacttcttga
    12001 tgatggcttt ggtaaaacag atcgctccgt gctggtgtgg atgccgctgg tggtgatcgg
    12061 gctgatgatt ttacgtggta tcaccagcta tgtctccagc tactgtatct cctgggtatc
    12121 aggaaaggtg gtaatgacca tgcgtcgccg cctgtttggt cacatgatgg gaatgccggt
    12181 ttcatttttt gacaaacagt caacggggac gctgttgtca cgtatcacct acgattccga
    12241 acaggttgct tcttcttctt ccggcgcact gataacggtt gtgcgtgaag gtgcgtcaat
    12301 catcggcctg ttcatcatga tgttctatta cagttggcaa ctgtcgatca ttttgattgt
    12361 gctggcaccg attgtttcga ttgcgattcg cgtagtatcg aagcgttttc gcaacatcag
    12421 taaaaacatg cagaacacca tggggcaggt gaccaccagc gccgaacaaa tgctgaaggg
    12481 ccacaaagaa gtattgattt tcggtggtca ggaagtggaa acgaaacgct ttgataaagt
    12541 cagcaaccga atgcgtcttc aggggatgaa aatggtttca gcctcttcca tctctgatcc
    12601 gatcattcag ctgatcgcct ctttggcgct ggcgtttgtt ctgtatgcgg cgagcttccc
    12661 aagtgtcatg gatagcctga ctgccggtac gattaccgtt gttttctctt caatgattgc
    12721 actgatgcgt ccgctgaaat cactgaccaa cgttaacgcc cagttccagc gcggtatggc
    12781 ggcttgtcag acgctgttta ccattctgga cagtgagcag gagaaagacg aaggtaagcg
    12841 cgtgatcgag cgtgcgactg gcgacgtgga attccgcaat gtcaccttta cttatccggg
    12901 acgtgacgta cctgcattgc gtaacatcaa cctgaaaatt ccggcaggga agacggttgc
    12961 tctggttgga cgctctggtt caggtaaatc aaccatcgcc agcctgatca ctcgttttta
    13021 cgatattgat gaaggcgaaa tcctgatgga tggtcacgat ctgcgcgagt ataccctggc
    13081 gtcgttacgt aaccaggttg ctctggtgtc gcagaatgtc catctgttta acgatacggt
    13141 tgctaacaac attgcttacg cacggactga acagtacagc cgtgagcaaa ttgaagaagc
    13201 ggcgcgtatg gcctacgcca tggacttcat caataagatg gataacggtc tcgatacagt
    13261 gattggtgaa aacggcgtgc tgctctctgg cggtcagcgt cagcgtattg ctatcgctcg
    13321 agccttgctg cgtgatagcc cgattctgat tctggatgaa gctacctcgg ctctggatac
    13381 cgaatccgaa cgtgcgattc aggcggcact ggatgagttg cagaaaaacc gtacctctct
    13441 ggtgattgcc caccgcttgt ctaccattga aaaggcagac gaaatcgtgg tcgtcgagga
    13501 tggtgtcatt gtggaacgcg gtacgcataa cgatttgctt gagcaccgcg gcgtttacgc
    13561 gcaacttcac aaaatgcagt ttggccaatg atcgaaaaaa tctggtctgg tgaatcccct
    13621 ttgtggcggc tattgctgcc actctcctgg ttgtatggcc tggtgagtgg cgcgatccgt
    13681 ctttgctata aactaaaact gaagcgcgcc tggcgtgccc ccgtaccggt tgtcgtggtt
    13741 ggtaatctca ccgcaggcgg caacggaaaa accccggtcg ttgtctggct ggtggaacag
    13801 ttgcaacagc gcggaattcg cgtgggggtc gtatcgcggg gatatggtgg taaggctgaa
    13861 tcttatccgc tgttattgtc ggcagatacc acaacagcac aggcgggtga tgaacctgtg
    13921 ttgatttatc aacgcactga tgcgcctgtt gcggtttctc ccgttcgttc tgatgcggta
    13981 aaagccattc tggcgcaaca ccctgatgtg cagatcatcg taaccgacga cggtttacag
    14041 cattaccgtc tggcgcgtga tgtggaaatt gtcgttattg atggtgtgcg tcgctttggc
    14101 aatggctggt ggttgccggc ggggccaatg cgtgagcgag cggggcgctt aaagtcagtt
    14161 gatgcggtaa tcgtcaacgg cggtgtcccc cgcagcggtg aaatccccat gcatctgctg
    14221 ccgggtcagg cggtgaattt acgtaccggt acgcgttgtg atgttgctca gcttgaacat
    14281 gtggtggcga tggcagggat tgggcatccg ccgcgctttt ttgccacgct gaagatgtgc
    14341 ggcgtacaac cggaaaaatg tgtaccgctg gccgatcatc agtctttgaa ccatgcggat
    14401 gtcagcgcgt tggtaagcac cgggcaaacg ctggtaatga ctgaaaaaga tgcggtgaaa
    14461 tgccgggcct ttgcagaaga aaattggtgg tatttgcctg tagacgcaca gctttcaggt
    14521 gatgaaccag cgaaactgct tacgcaacta acctcgctgg cttctggcaa ctagttacgc
    14581 cgcggcagcg ttcgattgat ggagtcatga atgtcgctgc cgcacctctc ccttgctgat
    14641 gcgcgtaatc ttcaccttgc cgcacaaggc ctgttaaaca aaccccgccg tcaagcgtcg
    14701 ttggaggata ttccggcaac gatctcccgc atgtccttgc tgcaaatcga taccatcaat
    14761 attgttgccc gtagtccata tctggtgctt ttcagtcgtc tgggaaatta tcctgcccag
    14821 tggctggatg agtctctggc gcgtggcgaa ttaatggaat actgggcgca tgaagcctgc
    14881 tttatgccgc gtagcgactt tcgtcttatt cgccaccgca tgctggcacc tgaaaaaatg
    14941 ggctggaaat acaaagacgc ctggatgcag gaacatgagg cggaaattgc acagttaatt
    15001 cagcatattc atgataaggg gccggtacgt tcagccgatt ttgagcatcc tcgtaaaggt
    15061 gcaagcggct ggtgggaatg gaagccgcat aaacggcatc tggaaggttt atttactgcc
    15121 ggaaaggtga tggtgattga acggcgcaac ttccagcgcg tttatgattt aacccaccgt
    15181 gtcatgcctg actgggatga tgagcgcgat ctcgtttcgc aaacagaagc agaaatcatc
    15241 atgctggata acagtgcgcg tagcctggga acattccgcg aacagtggct ggcagattac
    15301 tatcggctga aacgtccggc actggcggcg tggcgcgaag cgagggctga acagcagcaa
    15361 atcattgctg tgcatgttga aaaattgggc aatctttggc tgcatgctga tttgctgccg
    15421 ctactcgagc gtgcgctggc cggaaagctc actgcaacgc acagcgcggt actttcgcct
    15481 tttgatcctg ttgtctggga tcgcaaacgc gcagagcagc tttttgattt tagctaccgg
    15541 ctggagtgct ataccccagc gccgaaacgc cagtatggct attttgttct gccattatta
    15601 catcgtgggc aattagttgg gcgaatggat gccaaaatgc atcgccagac aggcatcctt
    15661 gaagttatct ctctgtggtt acaggaaggc attaaaccaa cgaaaatgct gcaaaaaggg
    15721 ttacgtcagg cgattactga tttcgcgagc tggcagcagg caacgcgggt gacattagga
    15781 cgctgcccgc aaggtctctt tactgattgc cgcactggct gggaaataga ccccgtcgca
    15841 taaatgatta tgataagatt tatgcattca taacccgatc catctggagg aactatggat
    15901 catcgtctgc ttgaaatcat tgcctgcccg gtttgcaacg gaaaactttg gtataaccaa
    15961 gaaaaacaag aacttatttg caaactggac aacctggctt tcccgctgcg tgatggcatt
    16021 ccagtgttgc tggaaacgga agcccgcgtg ctgactgctg atgagagtaa atcatgagtt
    16081 ttgtggtcat tattcccgcg cgctacgcgt caacgcgtct gcccggtaaa ccattggttg
    16141 atattaatgg caaacccatg attgttcatg ttcttgaacg tgctcgcgaa tcaggtgccg
    16201 atcgcatcat cgtggcaacc gatcatgagg atgtcgcccg cgccgttgaa gccgctggcg
    16261 gtgaagtatg tatgacgcgc gccgatcacc agtcagggac agaacgcctg gcggaagttg
    16321 tcgaaaaatg cgcattcagc gacgacacgg tgatcgttaa tgtgcagggt gatgaaccga
    16381 tgatccctgc gacaatcatt cgtcaggttg ctgataacct cgctcagcgt caggtgggta
    16441 tggcgactct ggcggtgcca atccacaatg cggaagaagc gtttaacccg aatgcggtga
    16501 aagtggttct cgacgctgaa gggtatgcac tgtacttctc tcgcgccacc attccttggg
    16561 atcgtgatcg ttttgcagaa ggccttgaaa ccgttggcga taacttcctg cgtcatcttg
    16621 gtatttatgg ctaccgtgca ggctttatcc gtcgttacgt caactggcag ccaagtccgt
    16681 tagaacacat cgaaatgtta gagcagcttc gtgttctgtg gtacggcgaa aaaatccatg
    16741 ttgctgttgc tcaggaagtt cctggcacag gtgtggatac ccctgaagat cttgagcgcg
    16801 ttcgcgctga aatgcgctaa tttcacttca cgacacttca gccaattttg ggaggagtgt
    16861 cgtaccgtta cgattttcct caatttttct tttcaacaat tgatctcatt caggtgacat
    16921 cttttatatt ggcgctcatt atgaaagcag tagcttttat gagggtaatc tgaatggaac
    16981 agctgcgtgc cgaattaagc catttactgg gcgaaaaact cagtcgtatt gagtgcgtca
    17041 atgaaaaagc ggatacggcg ttgtgggctt tgtatgacag ccagggaaac ccaatgccgt
    17101 taatggcaag aagctttagt acgcccggaa aagcccgaca actggcatgg aaaaccacca
    17161 tgctggcaag aagtgggact gtccgtatgc cgactattta tggtgtgatg acgcatgaag
    17221 aacaccccgg ccccgatgtc ctgctactgg agcggatgcg tggtgtttcg gtggaggcac
    17281 cagcccgaac accagaacgc tgggaacaac tcaaagacca aatcgttgaa gccttactgg
    17341 cctggcaccg tcaggacagt cgcggttgcg tcggcgcggt cgacaatact caggaaaatt
    17401 tctggccctc atggtaccgg caacatgttg aagtgctatg gaccacgctc aatcagttca
    17461 ataacaccgg tctgacgatg caggataagc ggatcctgtt tcgcactcgc gaatgtctcc
    17521 cggcattatt tgaaggcttt aacgacaatt gtgtgctgat tcacggtaac ttctgtttac
    17581 gcagcatgtt gaaagattcg cgcagcgatc agttactggc gatggtcggg ccgggactaa
    17641 tgctttgggc accgcgagaa tacgaactgt tccgactaat ggataattct ctggcggaag
    17701 atttgctctg gagttacctg caacgcgcgc cagtggcgga gtcgttcatc tggcggcgtt
    17761 ggttgtatgt gttatgggat gaagttgcgc aactggttaa taccggacga tttagtcggc
    17821 gcaacttcga tctggcatca aaatcactct tgccgtggct cgcctgacga accttttagc
    17881 cactgccaga tacggccaag cgtttcatag ccaacacgat cgctatgcat caaccagacc
    17941 ggagaaggga ttgcccgttc ccacgggtta agcggcgagt cgatggccaa ctgattcgcc
    18001 ggggctggta gcggatttaa cccttcctgc tgaaaaaaga tcatcgcccg cggcagatgt
    18061 gatgcggagg tcaccagcag gaaaggggcg tcaccaatcg cctgtttcac tgccgcagct
    18121 tcttcttcgg tatcttttgg caaatccagg gtgataattt gctcgtgcgg cacgcccagc
    18181 gattgcgcaa ctctggcacc tacttccgct gtactcaccg tattggtttt tgctacgcct
    18241 cccgtgaaga tcagttttga tcccggattt tcgcgccata agcgaatacc ttcattcagg
    18301 cgtggcagac tgttattgat taaattagag ctcggtgccc actgtgggtt ccaggtataa
    18361 ccacctccga gcaccacgat atagtccact ttttgcgaat tattccatgt cggataggtg
    18421 ctttcgatgg gacgcagcag gcggtcggct accggttgca aactcaataa caagagtgcc
    18481 agccacccaa tgctaataaa aatcttcccg gttttttgaa aacggctaaa ccacagcagc
    18541 gccaggccag cgccaataat taacaacatc agcggaagtg gcagcaacat attgccaatc
    18601 acttttttca gtgtaaaaag catcctttct ggttcctttt ttaaccatat agcaagggat
    18661 cgccagcgat attacaccag acgggttcat tctcgccgtg gctgtgacaa aatagcgtct
    18721 ttatcagaaa acgtcttagc caaccggtgg agaaagcaat gcaggatcgc aattttgatg
    18781 atattgcgga aaagttttcc cgtaacattt acggcaccac caaagggcag cttcgacagg
    18841 ctattctgtg gcaggatctc gatcacgtgc tggcggaaat gggcccgcaa aaactgcgtg
    18901 tgctggatgc tggcggtggt gaagggcaga ccgcaatcaa aatggccgag cgtgggcatc
    18961 aggtcatttt atgcgatctt tctgcgcaga tgatcgaccg cgcaaaacag gcggcagaag
    19021 caaaaggtgt gagcgacaac atgcaattta tacattgcgc cgctcaggat gttgcttcgc
    19081 atttggaaac gcccgttgat ctgatattgt tccatgcggt gctcgagtgg gtggccgatc
    19141 cccgcagtgt attgcagacc ctgtggtcag tgttgcgtcc aggcggcgtg ttgtcgttaa
    19201 tgttctacaa tgcgcatggt ttgttgatgc ataacatggt cgccgggaat tttgattacg
    19261 tgcaggcggg aatgccgaaa aagaaaaaac ggacgctttc gccagattat ccacgcgacc
    19321 cggcgcaggt ttatctgtgg ctggaagaag ctggttggca aattatgggt aagacaggcg
    19381 ttcgcgtgtt tcatgattat ctgcgcgaga agcaccagca gcgcgactgc tatgaagcat
    19441 tacttgaatt agaaacgcgt tattgccgtc aggaaccgta tattaccctg gggcgttata
    19501 ttcatgtcac cgcgcgcaaa ccgcagagca aggataaagt atgagtgaat tttcccagac
    19561 agtccccgaa ctggttgcct gggccagaaa aaatgacttc tccatctcgc tgccggtaga
    19621 ccgactctct tttctgctgg cggttgccac gctgaacggc gagcgtctgg atggtgagat
    19681 gagtgaaggc gagctggtgg atgcattccg ccatgtgagt gatgcgtttg agcaaaccag
    19741 cgaaaccatc ggcgtgcgcg ccaataacgc gatcaacgac atggtgcgtc aacgtctgct
    19801 gaaccgcttt accagcgagc aggcggaagg gaacgcaatt taccgtctga cgccgctcgg
    19861 catcggcatt actgactact acatccgtca gcgcgagttt tctacgctgc gtctttctat
    19921 gcagttgtcg attgtggcgg gtgagctcaa acgcgcagca gatgccgccg aagagggcgg
    19981 tgatgaattt cactggcacc gtaatgtcta tgcgccactg aaatattcgg tagcagaaat
    20041 tttcgacagt atcgacctga cgcaacgtct gatggacgaa cagcagcagc aggtgaagga
    20101 cgatatcgcc cagttgctga acaaagactg gcgggcggcg atttccagct gtgaattgtt
    20161 gctttcggaa acttccggga cgctgcgtga attgcaggat acgctggaag cggcaggcga
    20221 caaattgcag gctaatctgt tgcgcattca ggatgcgacg atgacccatg acgatctgca
    20281 tttcgtcgat cgtctggtgt tcgatctgca gagcaaactc gatcgtatta tcagttgggg
    20341 ccagcaatcc atcgacttgt ggattggcta cgaccgccac gtacacaaat ttattcgtac
    20401 cgcgatcgat atggataaaa accgcgtctt tgctcagcgg ttacgtcagt cggtacaaac
    20461 ctattttgat gagccgtggg cgctaactta tgccaatgcc gatcgtctgc tggatatgcg
    20521 tgacgaagag atggcactgc gcgatgaaga agtgactggg gaacttcctg aggatctgga
    20581 atacgaagag tttaacgaga tccgcgaaca gctggcggcg atcatcgaag aacaacttgc
    20641 cgtgtacaaa accagacaag tgccgctgga tcttggtctg gtggtacgcg aatatctgtc
    20701 acagtatccg cgtgcacgtc actttgacgt tgcgcgtatt gttattgatc aggcggtacg
    20761 tcttggcgta gcgcaagcag atttcaccgg actgccagcg aaatggcagc cgattaatga
    20821 ttacggagcc aaggtacagg cgcatgtcat cgacaaatat tgaacaagtg atgccggtta
    20881 agctggcgca ggcgctggcg aatccgttat ttccggcgct ggatagcgcc ttacgttcag
    20941 gccgccatat tggcctcgac gaactggata atcatgcatt cctgatggat tttcaggaat
    21001 atctggaaga gttttacgcg cgttataacg ttgagcttat tcgcgcaccg gaagggttct
    21061 tctatttacg cccacgttcc accactctta tcccgcgttc cgtcttgtcg gaactggata
    21121 tgatggtcgg gaaaatcctc tgttatctct atctcagccc ggaacggctg gcgaatgagg
    21181 ggatttttac tcagcaggaa ctgtacgacg aactgctcac cctggccgat gaagcaaaac
    21241 tgctgaaact ggtgaacaac cgttcaaccg gttcagacgt tgaccgtcag aagttgcagg
    21301 agaaagtacg ttcttcgctc aaccgtctgc gtcgtttagg catggtgtgg tttatgggcc
    21361 acgacagcag caagtttcgc attaccgaat cggtgttccg cttcggagcc gatgtgcgtg
    21421 ctggcgacga tccccgtgaa gcacagcgtc gcctgattcg tgatggcgaa gcaatgccga
    21481 ttgaaaatca tctgcaactc aacgatgaaa ccgaagagaa tcagccagat agcggagagg
    21541 aagaataatg attgaacgcg gtaaatttcg ctcactgacg ctgattaact ggaacggctt
    21601 ttttgcccga acttttgacc ttgacgagct ggtcacgacg ctttctggcg gtaacggggc
    21661 gggtaaatcc accaccatgg cggcgttcgt tacggcgctg atccccgacc tgaccctgct
    21721 gcatttccgt aacactacgg aagccggggc caccagcggt tcgcgcgata aaggtctgca
    21781 cggtaagctg aaagcgggtg tctgttattc gatgctcgac accattaact cgcgccacca
    21841 gcgcgtggtg gtcggtgtgc gtctgcaaca ggttgccgga cgcgatcgta aagtggatat
    21901 caagccgttt gccattcagg gactgccgat gtcggtgcag ccgacacagc tggtgaccga
    21961 aaccctgaac gaacgccagg cgcgcgtgct gccgcttaac gagctgaaag acaagctcga
    22021 ggcgatggaa ggcgtgcagt ttaaacagtt caactccatt actgattacc actcgctgat
    22081 gttcgatctg ggcatcatcg cgcgtcgtct gcgctccgca tctgaccgta gcaaattcta
    22141 tcgtctgatc gaagcttcgc tgtatggcgg gatctccagt gccattaccc gttctctgcg
    22201 cgactacctg ttgccggaaa acagcggcgt gcgtaaagcg ttccaggaca tggaagcggc
    22261 gctgcgtgaa aaccgtatga cgctggaagc gattcgtgtc acccagtcgg accgtgacct
    22321 gtttaagcat ctgatcagcg aagctaccaa ctacgtggcg gcggactaca tgcgtcacgc
    22381 caacgagcgc cgtgtccatc tcgacaaagc cctggagttt cgtcgcgagt tacatacttc
    22441 gcgtcagcaa ctggcggctg agcagtacaa acacgtcgat atggcgcgtg agctggcaga
    22501 gcacaacggt gccgaaggcg atctggaagc ggattatcag gcggccagcg atcacctgaa
    22561 cctggtgcaa accgcactgc gtcagcagga gaagatcgaa cgctacgaag cggatctcga
    22621 tgagctgcag atccgtctgg aagagcaaaa cgaagtggtg gcagaagcca tcgaacgcca
    22681 ggaagagaac gaggctcgcg cggaagctgc cgaactggaa gtggacgagc tgaaaagcca
    22741 gcttgctgac taccagcagg cgctggacgt ccagcaaacg cgcgcgatcc agtataacca
    22801 ggcgattgct gcgcttaatc gcgccaaaga actgtgccat ctgccggact taaccgccga
    22861 cagcgccgcc gaatggctgg aaaccttcca ggcgaaagag ctggaagcga ctgaaaaaat
    22921 gctctctctt gagcagaaaa tgagcatggc gcaaaccgcg cacagccagt ttgagcaggc
    22981 ttatcagctg gtggtggcaa tcaacggccc gctggcgcgt aacgaggcgt gggatgtcgc
    23041 tcgcgaacta ttgcgcgaag gggtcgatca gcgtcacctg gcagagcagg ttcaaccgtt
    23101 gcggatgcgc ttaagtgaac tggaacagcg tctgcgcgag cagcaagaag ccgaacgcct
    23161 gctggcagac ttctgcaaac gtcagggcaa gaattttgat attgatgaac tggaagctct
    23221 gcatcaggag ctggaagcgc gcattgcctc tctttccgat agcgtgtcta acgcccgtga
    23281 agagcgcatg gcactgcgcc aggaacagga acagctgcag tctcgcattc agagtttgat
    23341 gcagcgtgcg ccggtttggc tggcagcgca aaacagtctc aaccagttga gcgagcagtg
    23401 cggtgaagag tttacctcca gccaggacgt caccgaatat ctgcaacagt tgctggagcg
    23461 tgagcgagag gcgattgttg aacgcgatga agtgggcgcg cgcaaaaacg ctgtcgatga
    23521 agagatcgaa cgtttaagcc agcctggcgg ctctgaagat cagcgtctga acgcgctggc
    23581 ggagcgtttt ggcggcgtgc tgctgtcaga aatttatgac gacgttagcc tggaagatgc
    23641 gccgtacttc tcagcgctgt atggcccgtc acgccacgcc atcgtggtgc cagatctgtc
    23701 acaggtaact gaacacctgg aaggcttgac cgattgcccg gaagatctct atttgatcga
    23761 aggggatccg cagtcattcg atgacagcgt gttcagcgtt gatgagctgg aaaaagcggt
    23821 agtggtgaaa atcgccgatc gtcagtggcg ttattcacgt ttcccggaag tgccgctgtt
    23881 tggtcgtgct gcgcgtgaaa gccgtattga aagcctccat gccgagcgtg aagtgctttc
    23941 cgaacgcttc gccacgctct cctttgatgt acagaaaact cagcgtctgc atcaggcgtt
    24001 cagccgcttt atcggcagtc atctggcggt tgcgtttgag tctgacccgg aagcagaaat
    24061 ccgtcaactg aacagccgtc gcgtcgaact ggagcgggcg ttaagtaatc atgaaaatga
    24121 taaccagcag cagcgtattc agtttgagca ggcgaaagag ggcgttacgg cgctgaaccg
    24181 cattctgccg cgtctcaacc tgttggctga tgacagcctg gcggatcgcg tcgatgaaat
    24241 ccgcgaacgt ctggatgaag cccaggaagc cgcgcgtttt gttcagcagt ttggcaatca
    24301 actggcgaaa ctggaaccga ttgtttcggt attgcagagc gacccggaac agttcgaaca
    24361 gttaaaagaa gattacgcgt actctcagca gatgcagcgc gatgcccgtc agcaggcgtt
    24421 tgccctgacg gaagtggtgc agcgtcgtgc gcactttagc tattctgact cggcagaaat
    24481 gcttagcggt aacagcgatc tcaacgaaaa actgcgtgaa cgtctggaac aggcggaagc
    24541 ggagcgtacc cgcgctcgcg aagcgttgcg cggtcacgca gcgcagttga gtcagtacaa
    24601 ccaggtgctg gcttcgctga aaagttctta cgacaccaaa aaagagctac tcaacgatct
    24661 gcaacgtgaa ttgcaggata tcggcgtgcg tgctgatagc ggggcagaag agcgggcgcg
    24721 tattcgccgt gacgagctgc atgcgcaact gagcaataac cgttcacgcc gcaatcaact
    24781 ggaaaaagcg cttaccttct gcgaagcgga gatggacaac ctgacccgca aactgcgcaa
    24841 gctggagcgg gattactttg agatgcgcga gcaggtagtg accgccaaag cgggctggtg
    24901 tgcggtgatg cgcatggtga aagataacgg cgttgagcgc cgcttacacc gtcgtgagct
    24961 ggcttatctc tccgctgatg atttgcgctc tatgtcggat aaggcattgg gtgcgctgcg
    25021 tctggcggtg gcggataacg aacatctgcg cgacgtgctg cgcatgtcgg aagatccgaa
    25081 acgtccggag cgtaaaattc agttcttcgt ggcggtttat cagcatctgc gtgaacgtat
    25141 tcgtcaggat attattcgta ccgatgatcc ggtggaagct atcgaacaga tggagatcga
    25201 acttagccgt ctgaccgaag aattaacctc ccgtgaacag aaactggcga tcagttcccg
    25261 cagcgtggcg aacatcattc gcaaaaccat tcagcgcgag cagaaccgta tccgtatgct
    25321 caaccagggg ttgcagaacg tatcgtttgg tcaggtgaac agcgtgcgcc tcaacgtgaa
    25381 cgtgcgtgaa acgcacgcca tgctgctgga tgtgctctct gaacagcacg agcagcatca
    25441 ggatctgttt aacagcaacc gtctgacctt ctcggaagcg ttggcgaaat tgtatcagcg
    25501 tcttaatccg cagattgata tgggacagcg cacgccgcag accattggcg aagaactgct
    25561 ggattaccgc aactatctgg aaatggaagt tgaggttaac cgtggttccg atggttggtt
    25621 gcgcgcagag tctggtgcat tgtcgaccgg tgaggcgatt ggtaccggta tgtcgattct
    25681 ggtgatggtg gtacaaagct gggaagatga atctcgccgc ctgcgcggta aagatatctc
    25741 tccttgccgc ctgctgttcc tcgatgaagc agcgcgactg gatgctcgtt ctatcgccac
    25801 gctgtttgaa ttgtgtgagc gtttgcaaat gcagctcatc atcgcagcgc cggaaaatat
    25861 cagcccggag aaaggcacca cctataaact ggtgcgtaaa gtcttccaga ataccgaaca
    25921 cgttcacgtc gtcggcctgc gaggatttgc gccgcaactc cctgaaacgc ttccaggaag
    25981 tgacgaagcg ccttctcagg cgagttaaaa ttaaggcggc agcaatgccg ccttttcttt
    26041 ttccgaaaac tccgtttccg cactaaaaaa gtggcacatt acggcgcgct tttctttaaa
    26101 cttctttaca ttaggttatg taaaaacgta tcggcgttta tatactgaag ataagcctga
    26161 tgagtaacag gcttgctcgt catactttcg tgagtattgg cgttgtacag gcaagtcgta
    26221 aaataacagc ctggctattc agagtatgat aaaaacaggg ggcaagggat gttgcttaat
    26281 atgatgtgtg gtcgtcggct gtcggcaatc agtttgtgcc tggccgtaac attcgctcca
    26341 ctgttcaatg cgcaggccga tgagcctgaa gtaatccctg gcgacagccc ggtggctgtc
    26401 agtgaacagg gcgaggcact gccgcaggcg caagccacgg caataatggc ggggatccag
    26461 ccattgcctg aaggtgcggc agaaaaagcc cgcacgcaaa tcgaatctca attacccgca
    26521 ggttacaagc cggtttatct taaccagctt caactgttgt atgccgcacg cgatatgcaa
    26581 cccatgtggg aaaaccgtga tgctgttaaa gccttccagc aacagctggc agaggtggcg
    26641 attgccggtt tccagccgca gtttaataaa tgggtagagt tactgaccga tcctggtgtt
    26701 aacgggatgg cacgcgacgt agtgctctct gatgcgatga tgggctatct ccatttcatt
    26761 gcaaatattc cggtcaaagg cactcgctgg ctatatagca gtaaacctta tgcacttgca
    26821 acgccgccgc tctcggtgat taaccaatgg cagctggcgc tggataaagg tcaattgcct
    26881 acgtttgttg caggactggc accgcagcat ccgcaatatg cggcgatgca tgaatcgtta
    26941 ctggccttac tcagtgacac caaaccgtgg ccccaactga ccggcaaagc aacgttgcgc
    27001 ccagggcagt ggagtaacga cgtaccggcg ttgcgcgaaa tattgcaacg cacaggcatg
    27061 ttggacgggg ggccgaaaat tactctacct ggcgatgaca cgccaactga cgcggtagtc
    27121 agcccatccg ctgttactgt tgaaacagca gaaactaagc cgatggacaa gcaaacgacg
    27181 tctcgtagta aacctgcgcc tgccgttcgt gccgcctacg ataatgaact ggtggaagcc
    27241 gttaaacgtt ttcaggcatg gcaaggattg ggggcagatg gtgctattgg cccggcaaca
    27301 cgtgactggt taaacgtaac gcccgcccag cgtgctggcg tgttggctct caacatccag
    27361 cgattgcgct tgctgccaac agagctttct accgggatca tggttaacat tccggcctat
    27421 tcgctggtct actatcagaa cggcaatcag gtgctggatt cgcgagtcat tgtcggtcgc
    27481 cccgatcgca aaacgccgat gatgagcagt gcccttaaca acgtagtggt aaacccgccg
    27541 tggaacgtac ctccaactct ggcacgcaaa gatattctgc caaaagtgcg caacgatccg
    27601 ggatatctcg aaagccatgg ctatacggtg atgcgcggct ggaacagcag agaagcgatt
    27661 gacccatggc aggttgactg gtctacaatc acggcctcga atttaccgtt ccgcttccag
    27721 caggctccag gcccacggaa ctcgctgggg cgctataaat tcaatatgcc gagttcagag
    27781 gccatttatt tgcatgacac gccgaaccac aatctgttca agcgtgatac acgcgcattg
    27841 agctcaggct gtgtacgagt gaataaagct tccgatctgg cgaatatgct gttgcaggat
    27901 gcaggctgga atgacaaacg tatttctgat gcgctgaagc agggtgatac acgttacgtc
    27961 aatattcggc agtcgattcc ggtgaatctc tactacctga cggcctttgt tggtgcagat
    28021 ggtcgtaccc agtatcgtac agatatttac aattatgatc tgcctgcgcg atccagctcg
    28081 caaatcgtat cgaaagcgga acaattaatc aggtaaatga agtagttcgg ggaaatcagt
    28141 tgtcgtaata attagcatga ttgggggcga ttctctgcag cccccgtcac tgctggggtt
    28201 gagtcatctt gacgtctgct ttacgggcgg ttaaggtgcc tcttgtgcgc cagaagtgca
    28261 tataaacgat aacattgacc tgtagacttg attatcatgg acaaattcga cgctaatcgc
    28321 cgcaaattgc tggcgcttgg tggcgttgca ctcggtgccg ccatcctgcc gacccctgcg
    28381 tttgcaacac tctctacccc acgcccgcgc attttgacac tcaataatct tcataccgga
    28441 gagtcaatca aagcggagtt tttcgatggc agaggctata ttcaggaaga attggcaaaa
    28501 cttaaccatt ttttccgcga ttaccgcgcg aacaaaataa agtccatcga cccaggatta
    28561 ttcgaccagt tgtatcgcct gcaagggttg ttaggcacgc gcaaaccggt gcaactcatt
    28621 tccggttatc gttctattga taccaacaat gaactacgcg cccgcagccg tggagtagcg
    28681 aagaaaagct atcacactaa aggccaggcg atggatttcc atattgaagg tatcgcgtta
    28741 agcaatattc gcaaagccgc gttatctatg cgcgcaggtg gtgtaggata ttatccacgt
    28801 agtaactttg tgcatattga taccgggcca gcacggcact ggtagtaatt gcttaacgaa
    28861 acaggggcag tatgaactat cgtattattc cggtcaccgc attctcccag aactgttcat
    28921 taatctggtg tgaacaaacc cgtctggccg cactggtcga tcctggcggc gatgcggaaa
    28981 aaatcaaaca ggaagttgat gacagcggcc tgacactgat gcagatcctg ctgacgcatg
    29041 gtcatctgga ccacgttggc gcagcggcgg aactggcgca acattacggc gtgccggttt
    29101 ttggcccgga aaaagaagat gagttctggc tgcaaggctt gcctgcgcaa agtcgtatgt
    29161 ttggtctgga agagtgccag ccgctgacgc cagatcgttg gctgaacgaa ggcgatacca
    29221 tcagcatagg gaatgtgact ttacaggtgt tacattgccc tgggcatacg ccgggtcatg
    29281 tcgtgttttt tgatgatcgg gcaaagctgc tgatttctgg cgatgttatt ttcaaaggcg
    29341 gagtagggcg cagtgacttc ccgcgtggcg atcataatca actgatttct tcaatcaaag
    29401 ataaattgct gccactgggg gatgacgtga catttattcc gggtcacgga ccattatcca
    29461 cacttggtta tgaacgcctg cataatccct tcctgcaaga cgaaatgccc gtctggtaag
    29521 gcacataaaa aagcccgctt ttaatgcggg cctggatttc tggcaaagtg cgctttgttt
    29581 atgccggatg cggcacgagc gccttatccg gcctacaaaa tcgtgcaaat tcaaaatatt
    29641 gcaggggacg cgtaggcctg ataagcgtag cgcatcaggc aatgttgcgt ttgtcatcag
    29701 tctcagcccg cttttcagcg ggcttcattg tttttaatgc ttacagcact gccacaatcg
    29761 cttcgcacag cggagccatg ttatctggtg tcatcccggc cacatttacg cgaccagaag
    29821 caaccgcata tacgccaaac tcttcgcgca gacgcagcac ttgttctttt gtcaggccac
    29881 tgaaggagaa catgccgttc tgtttgatga taaagctgaa gtcgcggttt gcgccttttt
    29941 cctgcagcgt attgacgaac aactgacgca tacgctgaat acgctggcgc atatcagtca
    30001 gctcttgttc ccaaatcgca cgtaacgcat cgttgctcag gatggtggca acaacagaag
    30061 cgccgtgtgc tggtgggtta gagtagttag cgcgaatcgc cgctttcatt tggctgaatg
    30121 cgcgatcaac ggtttcactg tcggcagcaa ccagagtaca agcgccaaca cgctcgttgt
    30181 acaggccaaa gtttttagag taggaactgg caacaatcag ctctttatgc atagccgcga
    30241 aagcgcgcag tccttcagca tcttcttcca gaccacgggc aaaaccctgg taagcgaagt
    30301 caaacagcgg taaccagcct ttctcaactg agagttgtgc cagtgtctgc cattgttcca
    30361 gcgtagggtc gataccggtt gggttatggc agcagccatg gaacagcact acgtcgccag
    30421 cctgagcttc gttcaggctg ttaatcagtg catcgaagtc aagggtgtgg ttttctgcat
    30481 cataataagc gtattcacga acttccagac ctgcagagtt aaagacgctc ttatggttcg
    30541 gccagcttgg gttgctcacc cacactcgct taacgctggt attttttgcc aggaaatcgg
    30601 cagctacgcg tagtgcgcca gtgccccccg gagtctgtgc cgtgcgagca cgtttgtcat
    30661 tgatcagggc gctaccttta ccaaacagca gttcctgagt gcagcgacca aattcaggga
    30721 tgccgtcaat gccgaggtaa tttttggtgg tttcattttc gagcagatac tgttcagcct
    30781 ttttcacgct ggtcaatacc ggggttttgc ccgtctcatc tttatagaca ccaatcccga
    30841 ggttaatttt gccgggacgt tcatcggcac gaaacagatc ggccaggccc agaatcgggt
    30901 cggcaggagc ggcggtaatg ttctcaaaca tgacgaggtt ccattatggt tacagaaggg
    30961 aagtccgcta tcagggtaac gggagattta caaaattcca actattactg atgaaaacgc
    31021 aggctgtttt tgcaagacgt gagattgctc tggaaggcat aaaaaaaaca ggaccaaagt
    31081 cctgtttttt cggcatttaa caaagaggtg tgctattaga actggtaaac gatacccaca
    31141 gcaacggtgt cgtcagaacc tacgcccagt ttgttgtcag aatcgatctg gttgatgatg
    31201 tagtcaacat aggtggacat gtttttgttg aagtagtagg ttgcgcccac ttcaaagtag
    31261 ttcaccagat caacatcacc gataccttct acgtctttcg ctttagattt ggtgtaagcg
    31321 atggacggac gcagaccgaa atcgaactgg tattgcgcaa ctaacagaac gtcttgcgtt
    31381 ttgttggcga agccgctggt gtttgtaaat ttattagtga tcggcgtagc gttacgggtt
    31441 tcaccgtagt tcgctgccag gtagatgttg ttcgcgtcgt acttcagacc agtagcccac
    31501 tgttcagctt ttttaccgtt gccaagaggt tgagcttctt gcaggttggt acggtcagct
    31561 gcaccataag caccaacgat accaaagcct tcgtattcgt agctgataga accgccaaca
    31621 ccgtcgccgt tagaacggcg tgcagtgtca cgctcgtttt tacccaggta ctgaacagcg
    31681 aagttcaggc catcaaccag accaaagaag ttggagttac gataggtagc aacgccgcca
    31741 acacgaccaa cgaagaagtc atcgctgtat gcagtatcac caccaaattc tggcagcata
    31801 tcggtgtaac ccagtgcatc ataaaccaca ccgtagttac ggccgtaatc gaaagaacca
    31861 acgtcagcgt atttaagacc cgcgaatgcc agacgcgttt tgttaccagt ttgagcgtca
    31921 gcgccttcag agttgttacc ctggaagtta tattcccact gaccataacc ggtcagatcg
    31981 gaattgattt gagtttcccc tttaaaacca agacgggcat aggtcatgtc gccattgcca
    32041 ccgtaactgt tttcaccgtt acccttggaa aaataatgca gaccaacagc tttaccgtac
    32101 agatctactt tgttgccatc tttgttatag atttctgcag cgtttgcagt acctgctact
    32161 aacagagcag ggacgatcac tgccagaata ttgcgcttca tcattattta ttaccctcat
    32221 ggtttttttt atgacacctg ccactgccgt caataagttc tgtcaataaa aatttacgga
    32281 actattgatg agagtttggt gtctttatgt gtctgcaggc atctttccat tcaaactaac
    32341 gtttc
//
LOCUS       DAZFRN010000044        31884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFRN010000044 DAZFRN010000000
VERSION     DAZFRN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.044"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(173..1060)
                     /gene="rarD"
                     /locus_tag="ACHKUB_001957"
     CDS             complement(173..1060)
                     /gene="rarD"
                     /locus_tag="ACHKUB_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO6654981.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1112..1579)
                     /gene="yigI"
                     /locus_tag="ACHKUB_001958"
     CDS             complement(1112..1579)
                     /gene="yigI"
                     /locus_tag="ACHKUB_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO6654982.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1744..2613
                     /gene="pldA"
                     /locus_tag="ACHKUB_001959"
     CDS             1744..2613
                     /gene="pldA"
                     /locus_tag="ACHKUB_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO6654983.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2746..4575
                     /gene="recQ"
                     /locus_tag="ACHKUB_001960"
     CDS             2746..4575
                     /gene="recQ"
                     /locus_tag="ACHKUB_001960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO6654984.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNSLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4639..5259
                     /gene="rhtC"
                     /locus_tag="ACHKUB_001961"
     CDS             4639..5259
                     /gene="rhtC"
                     /locus_tag="ACHKUB_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO6654985.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5321..5941)
                     /gene="rhtB"
                     /locus_tag="ACHKUB_001962"
     CDS             complement(5321..5941)
                     /gene="rhtB"
                     /locus_tag="ACHKUB_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO6654986.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6052..7074
                     /gene="pldB"
                     /locus_tag="ACHKUB_001963"
     CDS             6052..7074
                     /gene="pldB"
                     /locus_tag="ACHKUB_001963"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO6654987.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7082..7882
                     /gene="yigL"
                     /locus_tag="ACHKUB_001964"
     CDS             7082..7882
                     /gene="yigL"
                     /locus_tag="ACHKUB_001964"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO6654988.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7958..8857
                     /gene="bioP"
                     /locus_tag="ACHKUB_001965"
     CDS             7958..8857
                     /gene="bioP"
                     /locus_tag="ACHKUB_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO6654989.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8745..9698)
                     /gene="metR"
                     /locus_tag="ACHKUB_001966"
     CDS             complement(8745..9698)
                     /gene="metR"
                     /locus_tag="ACHKUB_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO6654990.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9816..12077
                     /gene="metE"
                     /locus_tag="ACHKUB_001967"
     CDS             9816..12077
                     /gene="metE"
                     /locus_tag="ACHKUB_001967"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO6654991.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12116..12931)
                     /gene="ysgA"
                     /locus_tag="ACHKUB_001968"
     CDS             complement(12116..12931)
                     /gene="ysgA"
                     /locus_tag="ACHKUB_001968"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO6654992.1"
                     /translation="MATTQQSGFTPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGQLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFV
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13193..13954
                     /gene="udp"
                     /locus_tag="ACHKUB_001969"
     CDS             13193..13954
                     /gene="udp"
                     /locus_tag="ACHKUB_001969"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO6654993.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14094..15521
                     /gene="rmuC"
                     /locus_tag="ACHKUB_001970"
     CDS             14094..15521
                     /gene="rmuC"
                     /locus_tag="ACHKUB_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO6654994.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15616..16371
                     /gene="ubiE"
                     /locus_tag="ACHKUB_001971"
     CDS             15616..16371
                     /gene="ubiE"
                     /locus_tag="ACHKUB_001971"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO6654995.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16385..16990
                     /gene="ubiJ"
                     /locus_tag="ACHKUB_001972"
     CDS             16385..16990
                     /gene="ubiJ"
                     /locus_tag="ACHKUB_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO6654996.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16987..18627
                     /gene="ubiB"
                     /locus_tag="ACHKUB_001973"
     CDS             16987..18627
                     /gene="ubiB"
                     /locus_tag="ACHKUB_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO6654997.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18706..18975
                     /gene="tatA"
                     /locus_tag="ACHKUB_001974"
     CDS             18706..18975
                     /gene="tatA"
                     /locus_tag="ACHKUB_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO6654998.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18979..19494
                     /gene="tatB"
                     /locus_tag="ACHKUB_001975"
     CDS             18979..19494
                     /gene="tatB"
                     /locus_tag="ACHKUB_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO6654999.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19497..20273
                     /gene="tatC"
                     /locus_tag="ACHKUB_001976"
     CDS             19497..20273
                     /gene="tatC"
                     /locus_tag="ACHKUB_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO6655000.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20315..21097
                     /gene="tatD"
                     /locus_tag="ACHKUB_001977"
     CDS             20315..21097
                     /gene="tatD"
                     /locus_tag="ACHKUB_001977"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO6655001.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21094..21582)
                     /gene="rfaH"
                     /locus_tag="ACHKUB_001978"
     CDS             complement(21094..21582)
                     /gene="rfaH"
                     /locus_tag="ACHKUB_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO6655002.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21749..23242
                     /gene="ubiD"
                     /locus_tag="ACHKUB_001979"
     CDS             21749..23242
                     /gene="ubiD"
                     /locus_tag="ACHKUB_001979"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO6655003.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23288..23989
                     /gene="fre"
                     /locus_tag="ACHKUB_001980"
     CDS             23288..23989
                     /gene="fre"
                     /locus_tag="ACHKUB_001980"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO6655004.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24181..25344)
                     /gene="fadA"
                     /locus_tag="ACHKUB_001981"
     CDS             complement(24181..25344)
                     /gene="fadA"
                     /locus_tag="ACHKUB_001981"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO6655005.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25354..27543)
                     /gene="fadB"
                     /locus_tag="ACHKUB_001982"
     CDS             complement(25354..27543)
                     /gene="fadB"
                     /locus_tag="ACHKUB_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO6655006.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27733..29064
                     /gene="pepQ"
                     /locus_tag="ACHKUB_001983"
     CDS             27733..29064
                     /gene="pepQ"
                     /locus_tag="ACHKUB_001983"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO6655007.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29064..29678
                     /gene="yigZ"
                     /locus_tag="ACHKUB_001984"
     CDS             29064..29678
                     /gene="yigZ"
                     /locus_tag="ACHKUB_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO6655008.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29717..31168
                     /gene="trkH"
                     /locus_tag="ACHKUB_001985"
     CDS             29717..31168
                     /gene="trkH"
                     /locus_tag="ACHKUB_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO6655009.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31180..31725
                     /gene="hemG"
                     /locus_tag="ACHKUB_001986"
     CDS             31180..31725
                     /gene="hemG"
                     /locus_tag="ACHKUB_001986"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO6655010.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7398 a         8129 c         8738 g         7619 t
ORIGIN      
        1 ttttgtcaaa tccttggtag atatacgatg tctctaggat ggagtaagta ctggggagtt
       61 ttgggggtac ttcctgtaac aaatattgtt ctttttctta tcttaatttt tttcaaaaaa
      121 agataaataa gtcagtgggt aggctcatta aacggcctac ccatgttaat tcttaacttt
      181 ttctacgctg cgtataaatc gcatccatca caaaaattgc cagcgccacc caaataaagg
      241 cgaaagtcac catcttatcg gcacccggtt tttcaccata aaacgtcaca gccagcagga
      301 acatcagcgt cgggccaatg tactggaaaa agcctaacgt tgagagacgc aggcgcgtgg
      361 cagcggcagt aaaacacaac agcggtacgg tagtgacaat accggcggcg atcagcagta
      421 aattcagcga catcgggttt tgccccatat ggctggttga gctgtcggca atagcaaaca
      481 ggtaaatcgc cgccacgggc agcagccaca tggtttcaat taacatgccg gtttgtgctt
      541 ctaccgcaat cttcttgcgc accaggccgt agaaggcaaa actaaatgcc agccccagcg
      601 cgataatagg tagcgaacca aaagtccaca gctggaccaa cacgccacat atcgccagaa
      661 tcaccgccag ccattgcatc cggcggaatc gctcgccgag gaaaatcatc cccagcacaa
      721 tgttcaccag cgggttaata aagtaaccaa ggctcgcttc cagcatatgg tgattgttca
      781 ccgcccagat aaacagtagc cagttgccac caatcagcac ggcagagact gccagcataa
      841 aaattttctg tggcgtctga atcagcgttt ttaaatagga ccactggcgg caaatgctca
      901 tcagcaccac cataaagaaa aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg
      961 gcacgtagta aatcaacttg aagtacgctg gcgcaatacc ccaaataaaa taagcggcaa
     1021 gagcgagtaa tacgccctgc cgcgtttgtt ttgcatccat cgggaatact catttttaat
     1081 tggtaacagc agtttacctg cttttatgtc ttcaacctac catataggtg gcggtggcac
     1141 tggcaatata aagctgttct tcattgtgta attcaacgcg ggcgacggcg actttattgc
     1201 ctgcacgcaa caggctactg gtagcagtaa aacgctcgcc cctgcctggg cgcagataat
     1261 caacgcgaag atcaatggtc cccatccgcg atagccgctg gcgtagttca tcttcactga
     1321 tggtttcgtg gcgggttaag gtacttccta cgcacaccag accggcggcg acatccagcg
     1381 ccgacgcaat gaccccgccg tgcaaaatgc tttgcgccca gttgcccacc atcattggct
     1441 gatttttaaa ggccagctgt gcgaactctt tttcgtaacg ctccagttcc atccccaatg
     1501 cgcggttaaa tggcatgtga taaacaaaca tctcacccac taatttcagg gcttgttcag
     1561 cggtcagtac ggcagacata tcatccttac acttcattgg ttaatgaaat gttgatttta
     1621 tgcttctttg ttgttggttt ctactttagg aagggataac taacggcaat ggagttaagt
     1681 atgtaaaata gcccgcagaa aaacattcac cttatcaata attcgttacg gagaacacga
     1741 ccgatgcgga ctctgcaggg ctggttgttg ccggtgttta tgttgcctat ggcagtatat
     1801 gcacaagagg caacggtgaa agaggtgcat gacgcgccag cggtgcgtgg cagtattatc
     1861 gccaatatgc tgcaggagca tgacaatccg ttcacgctct atccttatga caccaactac
     1921 ctcatctaca cccaaaccag cgatctgaac aaagaagcga ttgccagtta cgactgggcg
     1981 gaaaatgcgc gtaaggatga agtaaagttt cagttgagcc tggcatttcc actgtggcgt
     2041 gggattttag gcccgaactc ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa
     2101 ctgtccaata gcgaagagtc ttcaccgttt cgtgaaacca actacgaacc gcaattgttc
     2161 ctcggttttg ccaccgatta ccgttttgca ggttggacgc tgcgcgatgt ggagatgggg
     2221 tataaccacg actctaacgg gcgttcagac ccgacctccc gcagctggaa ccgcctttat
     2281 actcgcctga tggcagaaaa cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg
     2341 ggtaatactg acgataaccc ggatatcacc aaatatatgg gttactacca gcttaaaatc
     2401 ggctatcacc tcggtgatgc ggtgctcagt gcgaaaggac agtacaactg gaacaccggc
     2461 tacggcggcg cggaattagg cttaagttac ccgatcacca aacatgtgcg cctttatact
     2521 caggtttaca gcggctatgg cgaatcgctc atcgactata acttcaacca gacccgtgtc
     2581 ggtgtggggg ttatgctaaa cgatttgttt tgatgaatgg ttgagtggtt ggcaaatctg
     2641 gaatccagca tccaggatta ccctctcaga gactaaaagc attgcagttt ctcgcgcagg
     2701 cgctgaaaat agcgcctgtt tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa
     2761 gtgttgaatc tggagtccgg agctaaacag gttttacaag aaacctttgg ctaccaacag
     2821 tttcgccccg gccaggaaga aattatcgac actgtgcttt ccggtcgcga ttgcctggtc
     2881 gtcatgccca ccggtggcgg aaaatccctt tgctatcaaa ttcctgcctt attgctaaac
     2941 agccttaccg tggttgtttc accgctgatt tcgttgatga aagatcaggt ggatcaactg
     3001 caagccaacg gcgtggcggc ggcgtgcctt aactcgacgc aaacccgcga acagcaactt
     3061 gaagtgatga caggctgccg caccgggcaa attcgtctgc tttatatcgc cccggaacgc
     3121 ctgatgctgg ataactttct tgagcatctg gcgcactgga atccggtgtt attagccgtt
     3181 gatgaagcgc actgtatctc ccaatggggc cacgatttcc gcccggaata tgccgcgctc
     3241 ggtcagttgc gccagcggtt cccgacgctg ccgtttatgg cgctgaccgc cacagccgac
     3301 gacaccacgc gccaggatat cgtgcgcctg ctggggctga acgatccgct gattcaaatc
     3361 agcagttttg accgtccgaa tattcgctac atgctgatgg agaaattcaa accgctcgat
     3421 cagttgatgc gctacgtgca ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc
     3481 cgggcgaaag tagaagacac cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc
     3541 tatcatgccg ggctggaaaa taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat
     3601 gacctgcaaa ttgtggtggc gacggtggcg ttcggcatgg gcatcaataa accaaacgtt
     3661 cgcttcgtgg tccactttga tattccgcgc aatatcgaat cctattatca ggaaaccggt
     3721 cgcgccgggc gtgatggcct gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg
     3781 gcgtggctgc gccgttgtct ggaagagaag ccgcaggggc agttgcagga tatcgagcgc
     3841 cacaaactca atgcgatggg cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg
     3901 ctgaactatt ttggcgaagg gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat
     3961 ccgccgaaac aatacgatgg ttcaaccgat gctcagattg ccctttccac cattggtcgt
     4021 gtgaatcagc ggtttgggat gggttatgtg gtggaagtga ttcgtggtgc taataaccag
     4081 cgtatccgcg actatggtca tgacaaactg aaagtctatg gcatgggccg tgataaaagc
     4141 catgaacatt gggtgagcgt gatccgccag ctgattcacc tcggcctggt gacgcaaaat
     4201 attgcccagc attctgccct acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa
     4261 tcctctttgc aacttgccgt gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa
     4321 tcgttcggcg gcaactatga tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg
     4381 attgccgatg aaagtaatgt cccgccgtac gtggtgttta acgacgcaac cttgattgag
     4441 atggctgaac agatgccgat caccgccagc gaaatgctca gcgttaacgg cgttgggatg
     4501 cgcaagctgg aacgctttgg taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc
     4561 gacgacgaag agtagtcagc agcataaaaa agtgccagga tgaagactcc gtaaacgttt
     4621 cccccgcgag tcaaatgtat gttgatgtta tttctcaccg tcgccatggt gcacattgtg
     4681 gcgcttatga gtcccggtcc cgatttcttt tttgtctctc agaccgctgt cagtcgttcc
     4741 cgtaaagaag cgatgatggg cgtgctgggt attacctgcg gcgttatggt ttgggcaggg
     4801 attgcgttgc ttggcctgca tttgattatc gaaaaaatgg cctggctgca tacgctgatt
     4861 atggttggcg gtggcctgta tctctgctgg atgggttatc agatgctgcg cggcgcgctg
     4921 aaaaaagagg cggtttctgc acctgcgcca caggtcgagc tggcgaaaag tgggcgcagt
     4981 ttcctgaaag gtttactgac caatctcgct aatccgaaag cgattatcta ttttggttcg
     5041 gtattctcat tgtttgtcgg tgataacgtt ggcactaccg cgcgctgggg catttttgcg
     5101 ctgatcattg tcgaaacact ggcgtggttt accgtcgttg ccagcctgtt tgccctgccg
     5161 caaatgcgcc gtggttatca acgtctggcg aagtggattg atggttttgc cggggcgtta
     5221 tttgccggat ttggcattca tttgattatt tcgcggtgat gccagacgcg tcttcagagt
     5281 aagtcggata aggcgtttac gccgcatccg acattatttt tcacgcatgc ctcgccgatg
     5341 ctaacagtgc tcccaccagc ataaacaacg agccgaaaat cttattcagc gccttcatct
     5401 gctttggtcc tttaatccat agagcaatcc gttgagcaag ggtggcgtaa ccgatcatca
     5461 caataatatc gaccacaata gtggtgacgc cgagcacgat atactgcatc agttgcggct
     5521 gttgcggcat gatgaattgc ggaaatagcg ccgccagaaa cacaatactt ttgggattgg
     5581 tgagattcac aaaaactgcg cgctggaaca aatgtcgacg cgattgagta gaggccagcg
     5641 atttaaggtc aattgcacca gcggcgcgcc actgctggat tcccagccaa atcaagtaag
     5701 ccgcgcctgc ccacttcaac acttcaaacg caatcactga gcgggaaaat agcgtcccca
     5761 accccacgcc aaccagcaca atatgaatcg ccagtccggt ctgaagccca gcaatagacg
     5821 ccaccgcgcc gcgataaccg tggttgagcg aggtggtcat agtgttgatt gcaccagagc
     5881 ctggcgacag gcttaaaatg atcgatgtca gcaggtaggc aaaccaccat tctaaggtca
     5941 tgatgaactc ccggtgtgtc tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt
     6001 caggcacgct aaaaaaaacg attttacgtg gtttaagagg cagattaccc gatgtttcag
     6061 cagcaaaaag actgggaaac aagagaaaac gcgtttgctg cttttaccat gggaccgctg
     6121 actgatttct ggcgtcagcg tgatgaagca gagtttactg gtgtggatga cattccggtg
     6181 cgctttgtcc gttttcgcgc acagcaccat gaccgggtgg tagtcatctg cccggggcgt
     6241 attgagagct acgtaaaata tgcggaactg gcctatgacc tgttccattt ggggtttgat
     6301 gtcttaatca tcgaccatcg cgggcaggga cgttccggtc gcctgttagc cgatccgcat
     6361 ctcgggcatg ttaatcgctt taatgattat gttgatgatc tggcggcatt ctggcagcag
     6421 gaggttcagc ccggtccgtg gcgtaaacgc tatatactgg cacattcgat gggcggtgcg
     6481 atctccacat tatttctgca acgccatcca ggtgtatgtg acgccattgc gctaactgcg
     6541 ccaatgtttg ggatcgtgat tcgtatgccg tcatttatgg cacggcagat cctcaactgg
     6601 gccgaagcgc atccacgttt ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg
     6661 ttgccgtttg ctatcaacgt actgacccac agcagacagc gatatcgacg taacttacgc
     6721 ttctatgctg atgacccaac gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa
     6781 agtattctgg ctggcgaaca ggtgttagcc ggtgcgggtg atgacgccac gccaacgctt
     6841 ctcttacaag ctgaagagga acgcgtggtg gataaccgca tgcatgaccg tttttgtgaa
     6901 ctccgcaccg ccgcgggcca tcctgtcgaa ggaggacggc cgttggtaat taaaggtgct
     6961 taccatgaga tcctttttga aaaggacgca atgcgctcag tcgcgctcca cgccatcgtt
     7021 gattttttca acaggcataa ctcacccagc ggaaaccgct ctacagaggt ttaaatttct
     7081 tatgtaccag gttgttgcgt ctgatttaga tggcacgtta ctttctcccg accatacgtt
     7141 atccccttac gccaaagaaa ctctgaagct gctcaccgcg cgcggcatca actttgtgtt
     7201 tgcgaccggt cgtcaccacg ttgatgtggg gcaaattcgc gataatctgg agattaagtc
     7261 ttacatgatt acctccaatg gtgcgcgcgt tcacgatctg gatggtaatc tgatttttgc
     7321 tcataacctg gatcgcgaca ttgccagcga tctgtttggc gtagtcaacg acaatccgga
     7381 catcattact aacgtttatc gcgacgacga atggtttatg aatcgccatc gcccggaaga
     7441 gatgcgcttt tttaaagaag cggtgttcaa atatgcgctg tatgagcctg gattactgga
     7501 gccggaaggc gtcagcaaag tgtttttcac ctgcgattcc catgaacaac tgctgccgct
     7561 ggagcaggcg attaacgctc gctggggcga tcgcgtcaac gtcagtttct ctaccttaac
     7621 ctgtctggaa gtgatggcgg gcggcgtgtc gaaaggccat gcgctggaag cagtggcgaa
     7681 gaaactgggc tacagcctga aggattgtat tgcgtttggt gacgggatga acgacgccga
     7741 aatgctgtcg atggcgggga aaggctgcat tatgggcagt gcgcaccagc gtctgaaaga
     7801 ccttcatccc gagctggaag tgattggtac taatgccgac gacgcggtgc cgcattatct
     7861 gcgtaaactc tatttatcgt aatcgttctt tatttggtca gttgtcaacc tgatacttcg
     7921 ctacaatgga tactcgttaa tcaaagagtt ttccattgtg gcgctactta tcatcaccac
     7981 gattctgtgg gccttctcct ttagctttta tggcgagtac cttgcggggc acgtcgatag
     8041 ctattttgcg gtgctggtgc gcgttggcct ggcggcactc gtttttctgc cgtttctgcg
     8101 tacccgtggc aatagcctga aaacggtcgg cctgtatatg ttggtgggcg cgatgcagct
     8161 cggcgtgatg tatatgctga gtttccgcgc gtatctctac ctgacggtct ccgaactgct
     8221 gctgttcacc gtactgacgc cgctctacat cacgctgatt tatgacatca tgagtaagcg
     8281 ccgtctgcgc tggggctatg cttttagcgc cttgctggcg gtgattggtg ccgggattat
     8341 tcgctatgat caggtcaccg accatttctg gactggcttg ttgctggtgc aactctccaa
     8401 tatcactttt gccatcggca tggtgggtta caaacgcctg atggaaactc gcccgatgcc
     8461 gcagcataac gcctttgcgt ggttctatct tggcgcgttc ctggtggcgg taattgcatg
     8521 gttcttgctg ggaaatgcgc agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt
     8581 gtttcttggc gtggtggctt ccgggattgg ctactttatg tggaactacg gcgcgacgca
     8641 ggtggacgcc ggaacgctgg gaattatgaa taatatgcac gttccggcag ggctgctggt
     8701 aaacctggct atctggcacc aacagccgca ctggccaacg tttattacag gcgcgctggt
     8761 gatcctggcc tcactgtggg tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc
     8821 agatgatcgc aggcgtgatt gcgcgctgag cgaataaacg cttccgtaac tggctgacgc
     8881 tgctcgccat cgcgcacggc ggcgtacagt cggctccaca agccttcgcc cagggttttg
     8941 gtcaccacca gaccctggcg ctcaaaactc tctactaccc aatgcggtag cgcggcaata
     9001 cccatccgcg cggcaaccat ctgaatcaac aataaggtgt tatcgacgct tttcagtgac
     9061 gggctgacgc ctgccggctg aagaaaatgc cgccagacat ccagtcgact acgctgcacc
     9121 ggataaatca acagcgtctc gctggcgaga tcttccggtg taattcgcgt tttcgccgcc
     9181 agtggatggt caggtgctaa caccagacgc acttcatagt cgaacatcgg cgaataatgc
     9241 aggccactgc gcggcaggat atcggaggtc atcaccagat ccagctcgcc ctgttgcaac
     9301 gcgggctgcg ggtcaaacgt cacgcccgat ttaaaatcca tctctacctg cggccagttc
     9361 ttatggaaat tttctaacgc gggcgtcagc cactgaatac agctatggca ctcaatggca
     9421 atgcgcagac gcgtctgctg cggttcattg caggcttgca gtgcctggct aatttgcggc
     9481 agcacctggt ttgccagttg caacaggatt tctccctgcg gtgtaaagcg tagcggctgg
     9541 ctcttacgca caaatagccg gaagccaagg cgttgttcca gatcgctaaa ctggtgagac
     9601 agggcggatt gcgtctgatg caacgtcgcc gcagcggctg cgagagagcc gcagttccgc
     9661 aacgcttgta gcgttttcag gtgttttact tcgatcatga aagtccttca cttcggcatg
     9721 aataatttgc gcttgaggaa tatacagtaa ccgccaatta tggatgtgta aacatctgga
     9781 cggctaaata ctaaaaattc cataaggggc atataatgac aattcttaat cacaccctcg
     9841 gtttccctcg cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg
     9901 ggaactccac gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg
     9961 atcaacaaaa gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc
    10021 atgttctgac caccagtctg ctgctgggta atgttccgcc acgtcatcag aacaaagacg
    10081 gttcggtaga tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac
    10141 ctgcggcggc agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg
    10201 aattcgttaa aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg
    10261 aggcgctggc gctgggccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt
    10321 ggctggggaa agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc
    10381 cggtttatca gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg
    10441 atgaacccgc gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt
    10501 acgacgcgct ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc
    10561 caaatctcga cacgattact gcgctgcctg ttcagggtct gcatgttgac ctcgtacatg
    10621 gtaaagatga cgttgctgaa ctgcacaagc gcctgccttc tgactggttg ctgtctgcgg
    10681 gtctgatcaa tggtcgtaac gtctggcgcg ccgatcttac cgagaaatat gcgcaaatta
    10741 aggacattgt cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc
    10801 ccatcgacct gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg
    10861 ccctacaaaa atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg
    10921 cagctctggc agagtggagc gccccgattc aggcacgtcg tcactctacc cgcgtacata
    10981 atccggcggt agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg
    11041 tctatgaagt gcgtgccgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca
    11101 ccacgattgg ttctttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa
    11161 agggcaatct cgacgccaat aactaccgca cgggcattgc ggaacatatc aggcaggcca
    11221 ttgttgaaca ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg
    11281 acatggtgga atactttggc gagcacctcg acgggtttgt ctttacgcaa aacggttggg
    11341 tacagagcta cggttcccgc tgcgtgaagc caccgattgt tattggtgac gttagtcgcc
    11401 cggcaccgat taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga
    11461 aagggatgct gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca
    11521 gccgtgaaac catcgccaaa cagattgcgc tggcgctgcg tgatgaggtg gccgatctgg
    11581 aagcggcagg gattggtatc atccagattg acgaaccggc gctgcgcgaa ggtttaccac
    11641 tgcgtcgtag cgactgggac gcctatctcc agtggggcgt agaggccttc cgtatcaacg
    11701 ccgccgtggc gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg
    11761 acatcatgga ttccattgcg gcgctggatg cagacgtcat caccatcgaa acctcgcgtt
    11821 ccgacatgga gttgctggag tcgtttgaag agtttgatta tccgaatgaa atcggtccag
    11881 gcgtctatga cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccctgctga
    11941 agaaagcggc aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga
    12001 aaacgcgcgg ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga
    12061 atttgcgtcg ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca
    12121 gcgacttctt cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg
    12181 cagattcggc atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt
    12241 acacgataat ctctgctttc gcattagcag cccgcagcgc ctggcgcatg gtttcaacgc
    12301 tctcctgcgg aatgctgtta tccagaccgc catataagcc gagaaccggc gcgttaagat
    12361 cggttgcgat atcaacaggt tgtttcggtg aattcagcga cttatcgccc gtcagtttgc
    12421 cgtaccacgc cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac
    12481 gtccacccca gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag
    12541 cccaactggc gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca
    12601 gaccgctaag caacgtgggg atatcgacaa aatcattcgg atcgccttcg cggaagtaaa
    12661 gttcaggtgc gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat
    12721 gttcatgcac gccaaaaatt tcctgcacta caatgaccac tggcagttgg ccatcgcttt
    12781 gctttggtct ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc
    12841 ccgccacaat cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag
    12901 gtgtaaatcc agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt
    12961 gcgcaatgtt aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt
    13021 tatgcagtcc tgcagaatga agagtgattt atgtgatttg aatcactttt ggtgggtaaa
    13081 tttatgcaac gcatttgcgc catggtgatg agtatcacga aaaaatgtta aacccttcgg
    13141 taaagtgtct ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa
    13201 gtctgatgtt tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat
    13261 cgtccctggc gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa
    13321 gctggcatct caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat
    13381 cgtctgctct accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca
    13441 gctgggcatt cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa
    13501 tgtgggtgat gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt
    13561 cgcaccgctg gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc
    13621 tgcgaaatct attggcgcga caactcacgt tggcgtgaca gcttcttctg ataccttcta
    13681 cccaggtcag gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc
    13741 tatggaagag tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct
    13801 gaccatgtgt gcaagccagg gcctgcgtgc cggtatggtg gcgggcgtta tcgttaaccg
    13861 cacccagcaa gagatcccga atgctgagac gatgaaacaa accgaaagcc aagcggtgaa
    13921 aatcgtggtg gaagcggcgc gtcgtctgct gtaatcctct tctcctgtct gaaggccgac
    13981 gcgttcggcc ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg
    14041 tttgtacagc acaattctat gttgtgcggg taagtaatga ggctggaggc gttgtggatt
    14101 tctcaatcat ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg
    14161 ccagttatca acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg
    14221 cggagttaag cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgtg
    14281 aattgctcaa taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc
    14341 tgcgtgaagt aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc
    14401 agatgattaa cagcgagcag cgcctcagtg agcagtttga aaacctcgct aaccgtattt
    14461 ttgagcacag caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt
    14521 cgccgctacg tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag
    14581 aagcacaaga acgccatacc ctgacccacg aaattcgtaa tctacagcaa ctcaacgcgc
    14641 aaatggccca ggaagcgatc aacctgacgc gcgcgctgaa aggcgataac aaaacccagg
    14701 ggaactgggg tgaagtggta ctgacgcggg tgctggaggc ttccggtctg cgtgaagggt
    14761 atgaatatga aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg
    14821 tcatcgtgcg gttgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg
    14881 cctatgaacg ctatttcaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac
    14941 atatcgcgtc ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc
    15001 cggggctgcg aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac
    15061 tggcgcttga ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg
    15121 ttagcccgac tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc
    15181 atcaaagccg caacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc
    15241 gtttgttcat cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact
    15301 accgccaggc aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag
    15361 cgtttcgcgg tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg
    15421 cggtgagcca ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag
    15481 cttatcaacg cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag
    15541 ccgggtagaa atctagggca tcgacgccca atctgttaca cttctggaac aattttttga
    15601 tgagcaggca ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc
    15661 gtcgcgaagg aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa
    15721 tacgatgtca tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg
    15781 attgattgca gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc
    15841 gacctgacag cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat
    15901 atcaatgaat ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc
    15961 aacgttgagt atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat
    16021 tgcatcacca tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca
    16081 atgtatcgcg tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt
    16141 atcgagccgc tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc
    16201 tcactggtcg cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat
    16261 cccgatcagg ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac
    16321 tacaatctga cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc
    16381 ggaaatgcct tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct
    16441 gtatcgctca cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt
    16501 ggaggtaaaa ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt
    16561 actgggcgaa tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc
    16621 gaaacttcgc gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca
    16681 gggcgatatt caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc
    16741 tgcggaactg ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat
    16801 gcgcggaggc gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga
    16861 agccattact gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga
    16921 agagacggct gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga
    16981 ggctaaatga cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc
    17041 tacggacttg atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga
    17101 tactcattat tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga
    17161 ctggccctgc aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc
    17221 cgcgatcttt ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct
    17281 ccgtttgatg gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta
    17341 gaagcgtggt ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt
    17401 cataccgcgc gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat
    17461 attttgccgg tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg
    17521 cgtttgctgc cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag
    17581 accttgattg atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc
    17641 aattttgaag acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa
    17701 gggatgatgg tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag
    17761 aaaaacggca ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag
    17821 gtctttcgcg acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat
    17881 gaacacccgg aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac
    17941 aaagaagata aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc
    18001 aaagtggcag agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag
    18061 ttcgaatttg ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt
    18121 tcgtttggac atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg
    18181 cagccgcaac tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag
    18241 ctttatccgc agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa
    18301 gatcaggtcg gtattcctgc cctggtgaga gcatttaaag aaaaagcgcc gttctgggtc
    18361 gaaaaaatgc cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta
    18421 cagcacagtg ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa
    18481 tcgcgttatt ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc
    18541 agccgacctg aatgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg
    18601 tttgtcggtt ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt
    18661 ataatgcggc tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt
    18721 atttggcagt tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc
    18781 ggctccatcg gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat
    18841 gatgaaccaa agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc
    18901 gataagcagg cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat
    18961 aaagagcagg tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc
    19021 atcggcctcg tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc
    19081 tggattcgcg cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta
    19141 aaactccagg agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg
    19201 acgcccgaac tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt
    19261 tcctacgttg caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg
    19321 gtggtgaaag ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag
    19381 gccagttcgc cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg
    19441 gacgctgaac cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt
    19501 ctgtagaaga tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga
    19561 actgcattat cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct
    19621 atcacctggt atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca
    19681 ccgacgtggc ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc
    19741 tgtcagcgcc ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc
    19801 atgaacgtcg cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca
    19861 tggcgttcgc ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc
    19921 cggaaggggt gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt
    19981 ttatggcgtt tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg
    20041 ggattacctc gccagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg
    20101 ttgtcgggat gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga
    20161 tgtactgtct gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa
    20221 atcgggaaga ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac
    20281 cgcccgtcag ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac
    20341 cagttcgcaa tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt
    20401 taatgggcta ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc
    20461 gcgtcagtat tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg
    20521 gcaagctgcg actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat
    20581 tggtgaatgt ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc
    20641 ttttgttgcc cagctacgca ttgccgcaga tttaaacatg ccggtattta tgcactgtcg
    20701 cgatgcccac gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc
    20761 ggttcttcat tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcatggaat
    20821 ttatatcggc attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt
    20881 gttgccgttg attccggcgg agaaattgct gatcgaaact gatgcgccgt atctgctccc
    20941 gcgcgatctc acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat
    21001 tttgcaacgt attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga
    21061 tgccaatgtc aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt
    21121 cacactgtgc ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc
    21181 atcgggttcg gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt
    21241 atcacccgga taaggggttg ccggatcgac aatgtctttc ggtttatata ccgatagctg
    21301 atgaatcacc gccgatggga ctatcgctgg cgacgcgcca aagcgcacga agtggctgac
    21361 accgcgggtc gcgttgatag tcgtggtatg aatcacttct ggatcaaatt cgacaaacag
    21421 gtagttggga aacaatggct cactgactgc agtacgtttt ccacgcacga ttttttccag
    21481 ggtgatcatc ggtgccaggc aattcacagc ctgtctttcg aggtgttcct gggcacgttg
    21541 aagttgcccg cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt
    21601 aatcggggcg caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg
    21661 ttataccgtc agagttcacg ctaatttaac aaatttacag catcgcaaag atgaacgccg
    21721 tataatgggc gtagattaag aggctacaat ggacgccatg aaatataacg atttacgcga
    21781 cttcttgacg ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc
    21841 gcatctggaa atcactgaaa ttgctgaccg cactttgcgt gccggtgggc ctgcgctgtt
    21901 gttcgaaaac cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa
    21961 gcgcgtggcg atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt
    22021 attggcgttt ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact
    22081 gccgcagttt aagcaagtat tgaacatgcc gacaaagcgg ctgcgtggtg cgccctgcca
    22141 acaaaaaatc gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg
    22201 gccggaagat gccgcgccgc tgattacctg ggggctgaca gtgacgcgcg gcccgcataa
    22261 agagcggcag aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat
    22321 gcgctggctg tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc
    22381 gggcgaacgt ttcccggttt ctgtggcgct gggtgccgat cccgccacga ttctcggtgc
    22441 agtcaccccc gttccggata cactttcaga gtatgcgttt gccggattgc tgcgcggtac
    22501 caagaccgaa gtggtgaagt gtatctccaa tgaccttgaa gtgcccgcca gtgcggagat
    22561 tgtgctggaa gggtatatcg aacaaggtga aacggcgccg gaagggccgt atggcgacca
    22621 caccggttac tataacgaag tcgatagttt cccggtattt accgtgacgc atattaccca
    22681 gcgtgaagat gcgatttacc actccaccta tactggtcgt ccgccggatg aacccgcggt
    22741 gctgggtgtc gcactgaacg aagtgtttgt gccgattctg caaaaacagt tcccggaaat
    22801 tgtcgatttt tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa
    22861 aaaacagtac gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca
    22921 gtttatgtac actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa
    22981 cgatgtgatt tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga
    23041 aaatacgcct attgattatc tggattttgc ctcgcctgtc tccgggctgg gttcaaaaat
    23101 ggggctggat gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat
    23161 caaaaaagat ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt
    23221 taacaacggt aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag
    23281 aaagcgcatg acaaccttaa gctgtaaagt gacctcggta gaagctatca cggataccgt
    23341 atatcgtgtc cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat
    23401 ggtagtgatg gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa
    23461 agggtttatc gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat
    23521 ggaccgcatc ctcaaagatc atcaaatcgt ggtcgatatt ccccacggag aagcgtggct
    23581 gcgcgatgat gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc
    23641 ccgctcgatt ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg
    23701 gggcgggcgt gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa
    23761 gcaccctggt ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg
    23821 tactggtacc gtgttaacgg cggtattgca ggatcacggt acgctggcag agcatgatat
    23881 ctatattgcc ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg
    23941 taatgcgcgg gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa
    24001 acccgcccct gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc
    24061 tatgtcgcat ccggcaatcg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg
    24121 cctacattgg tgcaggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg
    24181 ttaaacccgc tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag
    24241 accaaactga acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc
    24301 ggaacagccc agcggatgac ccagcgcgat cgcaccaccg ttgaggttga tcttctcgtc
    24361 aatctgctca atcagtccca gatctttaat acatggcagg atctgcgcgg caaaggcttc
    24421 gttcatctca aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt
    24481 cgaggccgga accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga
    24541 acgcacacga gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag
    24601 catggcagct gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac
    24661 tggatcaaac gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac
    24721 ttcgtcgtaa ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat
    24781 ttcattttta aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc
    24841 aaaggcatcc tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgctgttaa
    24901 gcccatcatg cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcaacgcc
    24961 gtgactcatc ggcacatggc ccatatgctc cacgccgcca accagacatg cctgcgcatc
    25021 gccggtcata atcatccgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg
    25081 attgacggta accgccggga cagagtgtgg tacttctgcc agcagcgccg cgttacgggc
    25141 gatgttaaaa ccctgctcca gcgtctgctg cacacaaccc cagtaaatat cgtcgagggc
    25201 cgccgcttcc agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc
    25261 ttctgcacgc acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc
    25321 atcgacaatg acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg
    25381 acgggctggc tcaaccgggg gatagtacgg ttcgttatga cgcgctttat tacgcagacc
    25441 ttccggcact tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt
    25501 tgcgctaccg agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag
    25561 gccgtagacc agcgccatat ccgcttccgc cggagtggcg ataatgcctt cctccagaca
    25621 gcgcaccact tcgttgacca tcgggatcat catgcgggcg ataatctcct cttcgctgaa
    25681 atcgcgcttc ggctggctca cttctgccag caggtcgtca acgacggcgt cttcttcttt
    25741 cttcggctta cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc
    25801 aaagcggttg gcatcaaaca gcgcgtcgat ggtatcgcga taatctttct gcatccgctg
    25861 cgggaagcct gctgccatga cagcctgggc atgatgcgcg gtatcaatgc ccacgacatc
    25921 cagcagatat gccggaccca taggccagcc aaactgtttt tccatcactt tgtcgatctt
    25981 gcggaaatcc gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac
    26041 gcggttaaca aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct
    26101 cgcccaggcg acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc
    26161 taccaacggc atccggtgta ccgggttaaa aaagtgcatt ccgcagaagt tttccgggcg
    26221 ttccagcgcg ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt
    26281 atccgggcgt actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc
    26341 gacaaccgct tctaccacaa catccacgcg gtcaaacccg gcgtagtcga gcgttgggtg
    26401 gattgtggag atcacgccag ccagtttcag accatcgatc ttgccgcgct caagctgctt
    26461 gttcagcagt ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt
    26521 catgacaacc ggcacgcctt tccacgcaga ctggtaagcg atgccgccgc ccataatgcc
    26581 tgcacccagc accgcggcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt
    26641 gccttttaca tattgatcgt taaggaaaat gccgaccagt gcgcgggctt cgttggtatg
    26701 cgccagcggg acaaaacttt tgttttccag gtttaaggct tcttcacgac caaaacgggc
    26761 cgcagcttca atggttttta ctgcggtgat gggggccgga taatgtttcc ccgctgtttg
    26821 tgcgaccatc cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag
    26881 tggttccagc ttcggctgac gttttgcttt ccagtcgagg tcgccgttaa tggcctggcg
    26941 taaaaccgcc tttgcgcctt caaccagttt ttctgctttg actacgccat ccaccagacc
    27001 gattttcagc gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagcgcact
    27061 gtcagcgccc agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag
    27121 tttggtttcc ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag
    27181 cacgcattcg cagccaccgc ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg
    27241 cagatcttcc aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc
    27301 ttcaggaacg aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc
    27361 tttgttcgaa cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc
    27421 ctcgccgagg ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa
    27481 taccagttcg gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag
    27541 catgtcagtc tcctgaatcc gcaaggtgat ctggtcgtac cagatgagtc gaagtgtgta
    27601 ttttgtgtta aaaatatgca aataaaagat taaagaaatg ccaatctgat cacgctcggc
    27661 agaaatcacg ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca
    27721 gaagggtaaa aaatggaatc actggcctca ctctataaaa atcatatagc taccttacag
    27781 gaacggactc gcgatgcgct ggcgcgcttc aagctggatg cgttacttat tcactccggc
    27841 gaactgttca acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc
    27901 aaagcgtggg tgccggtaac tcaggtgcca aactgctggc tgctggtgga tggcgtgaac
    27961 aagccgaaac tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg
    28021 acctctttct ggactgaaga cgtggaagtg atcgcgctgc cgaaagccga tggcattggt
    28081 agcctgctgc ctgctgcgcg cgggaatatc ggttatatcg gtccggtgcc ggaacgtgcg
    28141 ctgcaactgg gtattgaggc cagcaatatc aacccgaaag gggtgatcga ctacctgcat
    28201 tactaccgct ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg
    28261 gcggtcaacg gtcatcgcgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat
    28321 atcaacattg cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac
    28381 attgtggcgc tcaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca
    28441 ccggaagaga tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct
    28501 gacctgaccc gtacctggtc ggcaaaaagt gacaacgact acgcacagct ggtgaaagac
    28561 gtaaatgatg aacaactggc gctgatcgct accatgaaag caggcgtcag ctatgtggat
    28621 taccacatcc agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc
    28681 gatatgagtg aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt
    28741 attggccatc cgctgggcct gcaggtgcat gacgtagccg gttttatgca ggatgatagc
    28801 ggtacgcacc tcgcggcacc ggcaaaatat ccgtacctgc gctgcacccg tattctccag
    28861 ccgggcatgg tgttaaccat cgaaccgggt atctacttca ttgaatcgct gctggcaccg
    28921 tggcgtgaag ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg
    28981 ttcggcggca ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg
    29041 acccgggatc tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg
    29101 tcgttgaaga gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg
    29161 aggcggcgaa agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt
    29221 gcgtggcgtg ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg
    29281 gggagccggc gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg
    29341 gggaaattac cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt
    29401 tagtgaaagc gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca
    29461 agacgccatt aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag
    29521 cgttgctggg gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc
    29581 tgcgggtggc gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta
    29641 gccgtggttc attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac
    29701 taaggaagcg gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat
    29761 cttattttca gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc
    29821 gggccgcgct tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg
    29881 gccgaaccgc aaagagaaag gcgaacttaa atcccgtgag gggtttctga tagtggtgct
    29941 gttctggacc gtgctgggta gcgtcggtgc gctccctttt atcttctcgg aaagcccgaa
    30001 cctcacgatt accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac
    30061 tacgctggtg gggctggatt cgctccctca cgccatcctc ttttatcgcc agatgctgca
    30121 atggtttggc gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt
    30181 gggtgggatg cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg
    30241 cccgcgaatt gcggaaacgg cgaaaaccct gtggttgatt tatgtcttgc tgaccgtcgc
    30301 ctgtgcgctg gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt
    30361 tgcgactatc gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgatag
    30421 cccgactatt aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct
    30481 gcacttttca ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg
    30541 catgtttatc ggcgtacagt ttacgctggt ggttatttgt accctcgtac tgtggtttca
    30601 taatgtctac agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtgtcgat
    30661 ggcgacaacc gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt
    30721 actgctttta tgttcggctt ttatcggcgg ttgtgccggg tcaacgggcg gtggcctgaa
    30781 agtgatccgc atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca
    30841 tccgaacgcc gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga
    30901 agccgtttgg ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat
    30961 tatcgccacg ggcgtggatg acttttctgc ctttgcctca gttgttgcga cattgaataa
    31021 cctggggcca gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa
    31081 atggatcctg attgccaaca tgctgtttgg tcgtctcgag gtctttacat tgctggtgct
    31141 ctttaccccg actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc
    31201 tcaacaaggg acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa
    31261 ctggggatcc agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaat
    31321 tatgaccgtg tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag
    31381 gaatttgtca aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta
    31441 aacctggtgg cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag
    31501 tttctgatga actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt
    31561 tacccacgtt atcgctggta cgatcgtttt atgatcaagc taattatgaa gatgtcaggc
    31621 ggtgaaacgg atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc
    31681 gcccgagaaa tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa
    31741 aatgcgcggt cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata
    31801 atgcgccacc actgacacgg aacaacggca aacacgccgc cgggtcagcg gggttctcct
    31861 gagaatctca acagagaaaa gtaa
//
LOCUS       DAZFRN010000045        31437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFRN010000045 DAZFRN010000000
VERSION     DAZFRN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.045"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..108
                     /locus_tag="ACHKUB_001987"
     CDS             <1..108
                     /locus_tag="ACHKUB_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6655011.1"
                     /translation="GQERNLESISGYDKIVVKYNNWITKDNGEFELAVH"
     gene            138..638
                     /locus_tag="ACHKUB_001988"
     CDS             138..638
                     /locus_tag="ACHKUB_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO6655012.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            730..3415
                     /locus_tag="ACHKUB_001989"
                     /pseudo
     CDS             730..3415
                     /locus_tag="ACHKUB_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
     gene            3412..4500
                     /locus_tag="ACHKUB_001990"
     CDS             3412..4500
                     /locus_tag="ACHKUB_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO6655013.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4588..5886)
                     /gene="exuT"
                     /locus_tag="ACHKUB_001991"
     CDS             complement(4588..5886)
                     /gene="exuT"
                     /locus_tag="ACHKUB_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO6655014.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6369..7781
                     /gene="uxaC"
                     /locus_tag="ACHKUB_001992"
     CDS             6369..7781
                     /gene="uxaC"
                     /locus_tag="ACHKUB_001992"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO6655015.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7796..9283
                     /gene="uxaA"
                     /locus_tag="ACHKUB_001993"
     CDS             7796..9283
                     /gene="uxaA"
                     /locus_tag="ACHKUB_001993"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO6655016.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9366..9917
                     /gene="ygjV"
                     /locus_tag="ACHKUB_001994"
     CDS             9366..9917
                     /gene="ygjV"
                     /locus_tag="ACHKUB_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO6655017.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9922..11166)
                     /gene="sstT"
                     /locus_tag="ACHKUB_001995"
     CDS             complement(9922..11166)
                     /gene="sstT"
                     /locus_tag="ACHKUB_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO6655018.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(11565..12530)
                     /gene="alx"
                     /locus_tag="ACHKUB_001996"
     CDS             complement(11565..12530)
                     /gene="alx"
                     /locus_tag="ACHKUB_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO6655019.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(12813..13799)
                     /gene="ygjR"
                     /locus_tag="ACHKUB_001997"
     CDS             complement(12813..13799)
                     /gene="ygjR"
                     /locus_tag="ACHKUB_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6655020.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(13878..14561)
                     /gene="ygjQ"
                     /locus_tag="ACHKUB_001998"
     CDS             complement(13878..14561)
                     /gene="ygjQ"
                     /locus_tag="ACHKUB_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO6655021.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(14638..15141)
                     /gene="ygjP"
                     /locus_tag="ACHKUB_001999"
     CDS             complement(14638..15141)
                     /gene="ygjP"
                     /locus_tag="ACHKUB_001999"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO6655022.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15226..16362
                     /gene="rlmG"
                     /locus_tag="ACHKUB_002000"
     CDS             15226..16362
                     /gene="rlmG"
                     /locus_tag="ACHKUB_002000"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO6655023.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            16553..16636
                     /locus_tag="ACHKUB_002001"
                     /pseudo
     CDS             16553..16636
                     /locus_tag="ACHKUB_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            16633..17049
                     /gene="higA"
                     /locus_tag="ACHKUB_002002"
     CDS             16633..17049
                     /gene="higA"
                     /locus_tag="ACHKUB_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO6655024.1"
                     /translation="MIALSNILQAGEQLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMVEAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVSSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17094..19112)
                     /gene="fadH"
                     /locus_tag="ACHKUB_002003"
     CDS             complement(17094..19112)
                     /gene="fadH"
                     /locus_tag="ACHKUB_002003"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO6655025.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGETQVFAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19438..21789)
                     /gene="ygjK"
                     /locus_tag="ACHKUB_002004"
     CDS             complement(19438..21789)
                     /gene="ygjK"
                     /locus_tag="ACHKUB_002004"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO6655026.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFMVKKSDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21806..22876)
                     /gene="ygjJ"
                     /locus_tag="ACHKUB_002005"
     CDS             complement(21806..22876)
                     /gene="ygjJ"
                     /locus_tag="ACHKUB_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO6655027.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDETKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23010..24443)
                     /gene="ygjI"
                     /locus_tag="ACHKUB_002006"
     CDS             complement(23010..24443)
                     /gene="ygjI"
                     /locus_tag="ACHKUB_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6655028.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(24506..24955)
                     /gene="ebgC"
                     /locus_tag="ACHKUB_002007"
     CDS             complement(24506..24955)
                     /gene="ebgC"
                     /locus_tag="ACHKUB_002007"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO6655029.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24952..28044)
                     /gene="ebgA"
                     /locus_tag="ACHKUB_002008"
     CDS             complement(24952..28044)
                     /gene="ebgA"
                     /locus_tag="ACHKUB_002008"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO6655030.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28228..29211)
                     /gene="ebgR"
                     /locus_tag="ACHKUB_002009"
     CDS             complement(28228..29211)
                     /gene="ebgR"
                     /locus_tag="ACHKUB_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO6655031.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29430..29762
                     /gene="ygjH"
                     /locus_tag="ACHKUB_002010"
     CDS             29430..29762
                     /gene="ygjH"
                     /locus_tag="ACHKUB_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO6655032.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(29804..31183)
                     /gene="ygjG"
                     /locus_tag="ACHKUB_002011"
     CDS             complement(29804..31183)
                     /gene="ygjG"
                     /locus_tag="ACHKUB_002011"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO6655033.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
BASE COUNT         7763 a         8055 c         8192 g         7427 t
ORIGIN      
        1 ggtcaggaaa gaaatcttga aagcatatct ggctacgaca aaatcgttgt caaatataat
       61 aactggatca ccaaagataa tggcgagttt gaactggcgg tccattaatt atcattgtaa
      121 taaacgaagg aatagtaatg aaaaaggtat ttgcaaaatc tcttctggtc gcagcgatgt
      181 tttctgttgc aggctccgca ttggcagtac aaaaggatat tactgtaacg gccaacgttg
      241 atgccgctct ggatatgaca cagaccgata acaccgcgtt gccgaaagca gtagaaatgc
      301 aatatctgcc ggggcagggt ctgcaatctt accagttgat gaccaaaatc tggtctaacg
      361 acgttaccaa agatgtaaaa atgcagttag tttccccggc gcaactggtt caaagcctgg
      421 atgctagcaa gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg
      481 cggcaaccac ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc
      541 tggctaaaaa cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc
      601 gtggtgtagt aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg
      661 taacaaaggg aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc
      721 aggattgcca tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca
      781 gaggcgacat taaccaaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag
      841 tatatcgagg tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt
      901 gataccgtaa catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac
      961 gatcagaaaa tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcgccacggt
     1021 gaattggctt gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg
     1081 ctggaaataa tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg
     1141 aattcagctt tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct
     1201 tttatacatc agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt
     1261 cagggaaacg gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc
     1321 aacggttatg atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat
     1381 cgttatgatt ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta
     1441 tttaatgcac aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg
     1501 atgcgtatcg ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca
     1561 gtaatggttc tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg
     1621 ggatcgtttt atctcaatag tggttcgcaa tttattgata ccagttcctt tccgcctggc
     1681 agctacagcg tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg
     1741 ccgtttacca aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt
     1801 aaaactacat cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg
     1861 ccattacatc cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct
     1921 ttcgagttag gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc
     1981 gccagcgtgt tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt
     2041 aatccgggat ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca
     2101 accgacagca gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt
     2161 tcacagaatt ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact
     2221 gatgatagtt tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca
     2281 acagcgcaaa gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt
     2341 gactggattt tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac
     2401 gacgacaacg gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat
     2461 aacaacagcc attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat
     2521 caaacgtcat ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt
     2581 ggcgtaaccg tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg
     2641 gatgggcaat acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat
     2701 gatcatcttt cggcctttac gggcacttat agctctacgc tggctgtcag tcgctatggc
     2761 gttaatttgg gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa
     2821 ggcttctctg aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc
     2881 agccgaactt tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag
     2941 tctggttttg ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacaat
     3001 cattaacggt gcagggaatc gtgaattaat gttgttgcct ggcaagctgc gctatcgcga
     3061 agtgtctgcc agctttaact acaactatat cggtcgttta ttactgccgg cagcggtgaa
     3121 aaaattcccg attgtggggc tgaacagcgc catgttactg gtagctgaag atggcggatt
     3181 tacacttgaa attaacggca gcgaaaaaga gctctatctg ctttccgggc agcaattcct
     3241 taagtgtcca ctgagtgttg taaagaaacg cgccagcatt cgttacagcg gagatgttac
     3301 ttgtagtgtg gtgacttatt cacaattacc ggagtcgatt caggttcagg cacagttgaa
     3361 acagcctaaa ttacgtggaa acgttcagac ggcgcaaagg gaggttgcac catgagaaat
     3421 cgattgattg cggcgatatt gggcttgttt ggcacgctca ctggcgttca ggcagctcct
     3481 gacgtgacca gtgaaattac gtatgatttg gcatctggta gagcggatta ttacttctgg
     3541 aaggatgaag cgtcggcagg aaataatgga tatatgtggt atgaatgttc gtatcctgac
     3601 ctccaacaaa cctgtacagc taatggaaat atatcgacag tacaaatcta tttaactgaa
     3661 caacgcagtg ggatgcgttg gccggtaaaa ctcaaaggat ttaaaacagc cattgtaagt
     3721 agcgatgaag cgccgccagg atgcaagggg ggcaaagggc ttcagacgaa tcttaaggat
     3781 tctaatagat cttcatgtac agaagatggt caacattatt atatatacga tacaaagttt
     3841 cttacgctct accttgagca gacagagatg aagaatttgc cgattggtgg cgtctggaag
     3901 gggaaagtta aattacattc gaacagcccg gcccaggact atttcgcaaa tattaccctg
     3961 aatacgctcg accccaacca tattgacgtg ttcttcccgg agttcgccca cgccacgcca
     4021 agggtgcagt tagacttgca tccaacagga agcgttaacg gcagcaacta cgcgcaagat
     4081 ctgaccatgt tggacatgtg cctgtacgat ggttttaacg gtaatgccat cagttatgaa
     4141 atcatgctca aagatgaagg gcgaccagct gcagggcgca gagacggtta cttctctatc
     4201 tatcgtcagg gagggaccac caccgacgag ggagaacgca ttgattaccg ggtcaaaatg
     4261 tacaacccgg aaaccggtgg gcaaattgat gtgcgcaata atgaaaatat ggtctggaac
     4321 agcattaacc tgaaacgtgt gcgtccggtg gtactgcccg gtattcgcta tgccgtgatg
     4381 tgtgtgccga cacccttgac tctggcagtc gataaattca gcgtgatgga taaacaggcc
     4441 gggtactaca tgggcaaatt gtcagtaatc tttacgcctt ccttgccaac catcaattaa
     4501 ccctgcaaac cggacaaacg cttttttcta tataaacgac gaagccgtct gataaggcgg
     4561 cttcgtcatt acaacttaag ttaaacgtta atgttgcggt gcgggatcgt tatgtgtttc
     4621 ctgcgccacc tcaatcgccg gtttgttctg caacacggtc cagataacca gcgcacctaa
     4681 caggtcaaat actgccagaa ctgcgaacag cgggctgaag ccgatggtgt cagccagtgc
     4741 accgacaacc agcgcaaaca gcgtacttgc cagccatgcg gacatcccgg ttaaaccgtt
     4801 cgccgtcgcc acttcgttac gaccgaacac gtcagaagag agcgtaatca gcgcgccaga
     4861 cagtgcctgg tgggcaaaac caccgataca cagcagcata attgcgacat acgggttggt
     4921 gaacagaccg atcatacccg ggccaatcat cagcacagca cccagcgtta cgaccatctt
     4981 acgggaaacg atcaggttca caccaaacca acgctggaac agcggcggca ggtaaccacc
     5041 gaggatacaa ccgaggtcag caaacagcat cggcatccag gcgaacatcg cgatctcttt
     5101 caggttaaag ccgtaaactt taaacatgaa cagcgggatc cacgcgttaa aagtacccca
     5161 ggccggttct gccaggaaac gcggcagcgc gataccccag aactgacggt tacgcaggat
     5221 ctgaccaacg gacattttct tcgccgtgct cacctggtgc tgggcttcct gaccattaat
     5281 aatatagtcg cgttcttcat cggtcagatg cttctggtcg cgtggatgtt tatagaaaat
     5341 cagccatgcc atcgcccaga taaagctcaa tgcaccggag atgataaatg ccatctgcca
     5401 gctgtgcatt acgattgccc ataccaccag cggcggcgca atcatcgcac caatcgaaga
     5461 acctacgtta aagtaaccta ccgcgatgga acgctctttc gccggaaacc attcggagct
     5521 ggctttcaga cccgccggga tcatcgctgc ttccgcggca ccgactgcac cacgagcaat
     5581 cgccaggcca ccccagctac cagccagcgc ggttgcacca cagaacacgg cccacagcac
     5641 agcaaacatt gcataaccga ttttcgtacc cagcacatcc agtacataac ctgctaccgg
     5701 ttgcatgacc gtataagcag cagaataggc tgcgatgata taggaatact gttgggtgga
     5761 gatgtttaac tcttccatca gagttggcgc agctgccgcc acagtgttac gcgtcaggta
     5821 accaagcacg gtgccgagcg tcaccagtgc gatcatatac caacgtaacc ctttaatttt
     5881 acgcatgtaa aacctcatcg ttttgttata tccgcaggaa gcgcggccat catcccgcca
     5941 gaggctgaag atgttagtta cgaccgggta accgaaaaaa aaccggcacg ccccgaacgt
     6001 tgccatgaaa gtttcgtgct aattcggctt ccgtaccggt taacccgatt catctaacaa
     6061 atgaatgaat cggcaatttg cattccgtcg acttatgatt tgcgacggca gaaagataac
     6121 ttgtcataca actttaaaag gtgagagcca tcacaaatgt gcgaatattt gtagggacat
     6181 tacctgacga cagcgaggcc agtactggcg cggcctgcag agagatttac cactttgaga
     6241 gtaatttttt taactacgtt tattgatcta actcacgaaa atatcttcgg actctggaaa
     6301 ttggtgtgat aactttgtca gcatcgcacc ataagcaagc tagctcactc gttgagagga
     6361 agacgaaaat gactccgttt atgactgaag atttcctgtt agataccgaa tttgcccgcc
     6421 gtctgtatca cgactacgca aaagaccagc cgattttcga ttaccattgc catttgccgc
     6481 cgcagcagat tgcggaagac tatcgtttta aaaacctgta tgacatctgg ctgaaaggcg
     6541 atcactacaa atggcgcgct atgcgtacca acggtgtggc cgagcgtctg tgtaccggtg
     6601 atgcgtctga ccgtgaaaaa tttgacgcct gggcggcgac tgttccgcac actatcggca
     6661 acccgttata ccactggacg cacctcgaac tgcgtcgtcc gtttggtatc actggcaaat
     6721 tgctttctcc gtcaactgcc gatgaaatct ggaacgaatg taacgaactg ctggcgcagg
     6781 ataacttctc cgcgcgcggc atcatgcagc agatgaacgt gaaaatggtc ggcaccaccg
     6841 atgacccgat cgattctctg gagcatcacg cagagatcgc caaagacggc tctttcacca
     6901 tcaaagtgct gccgagctgg cgtccggaca aagccttcaa catcgaacag gcgaccttta
     6961 acgactacat ggcgaagctg ggcgaagttt ccgataccga cattcgccgc tttgctgacc
     7021 tgcaaactgc cttgaccaaa cgtctggatc acttcgccgc tcacggctgt aaagtgtctg
     7081 accatgcgct ggacgtggtg atgtttgctg aagcgaacga agcggaactg gacagcattc
     7141 tggcgcgccg tctggctggc gaacccctga gcgagcacga agtggcacag ttcaaaactg
     7201 cggtactggt gttccttggt gccgaatatg cacgtcgcgg ctgggtacag cagtaccaca
     7261 ttggcgcgct gcgtaataac aacctgcgtc agttcaaact gctggggccg gatgtaggct
     7321 ttgactccat caacgaccgt ccgatggcgg aagagttgtc taagctgctg agcaagcaga
     7381 acgaagaaaa cctgctgccg aaaaccattc tctactgcct gaacccgcgc gataacgaag
     7441 tgctgggcac catgatcggt aacttccagg gcgaaggtat gccgggcaaa atgcagttcg
     7501 gttccggctg gtggtttaac gatcagaaag acggtatgga acgtcagatg acccaactgg
     7561 cgcagctcgg tctgctgagc cgctttgtcg gtatgctgac tgacagccgt agcttcctgt
     7621 catacacccg tcacgaatac ttccgccgca ttctgtgcca gatgatcggt cgctgggtgg
     7681 aagcgggcga agcaccggcg gacatcaacc tgctgggcga gatggtgaaa aatatttgct
     7741 ttaacaatgc gcgtgactac ttcgccattg aactgaacta aggtctgggg ttgatatgca
     7801 atacatcaag atccatgcgc tggataacgt cgcggtcgct ttagcagatt tggctgaagg
     7861 cacagaagtc agtgtcgata accagactgt tacgctgcgc caggatgttg ctcgtggaca
     7921 taaatttgcg ttaacggata tcgcaaaagg ggccaacgtc atcaaatatg gcctgccgat
     7981 tggttatgca ttggcggata ttgcggcggg ggaacacgtc cacgcccaca atacgcgcac
     8041 gaatctgagc gatctggatc agtatcgcta tcaacctgat tttcaggatc tgcctgcgca
     8101 agcggcagat cgtgaagtgc agatctatcg tcgcgctaac ggcgatgtcg gggtgcgtaa
     8161 tgagctgtgg atcctgccaa ccgtgggctg tgtcaacggc atcgcgcggc aaatccagaa
     8221 ccgtttcctg aaagagacca acaacgccga aggtaccgac ggcgtgttcc tcttcagcca
     8281 cacctacggc tgctcacaac tgggcgacga tcacatcaac acccgcacca tgctgcaaaa
     8341 catggtgcgc cacccgaacg cgggcgcagt gctggtgatt ggtctgggct gtgaaaacaa
     8401 ccaggttgcc gcattccgtg aaacgttggg cgatatcgat cctgaacgcg ttcatttcat
     8461 gatctgccaa cagcaggatg atgagatcga agccgggatc gagcatttgc atcagctgta
     8521 taacgtgatg cgcaacgata aacgcgagcc aggcaaactc agcgaactga agtttggtct
     8581 ggagtgcggt ggttctgacg gtctttctgg tattactgct aacccgatgc tggggcgttt
     8641 ctctgactac gtgattgcta acggcggtac taccgtactg accgaagtgc cggagatgtt
     8701 tggcgcagag cagttgctga tggaccattg ccgcgacgaa gcaacgtttg aaaaactggt
     8761 caccatggtc aacgacttca aacagtactt tattgcccat gaccagccga tctatgaaaa
     8821 cccatcgccg gggaacaaag cgggcggtat caccacgctg gaagataaat cccttggctg
     8881 tacccagaaa gcgggttcca gcgtcgtggt tgacgtgctg cgttacggcg agcgtctgaa
     8941 aacgccgggg ctgaacttgt taagtgcgcc gggtaacgat gccgtagcga ccagcgccct
     9001 ggcgggtgcg ggctgccata tggtgctgtt cagtactggt cgtggcacgc catacggcgg
     9061 ctttgtaccg acggtgaaaa tcgctaccaa cagtgaactg gcggcgaaga aaaaacactg
     9121 gatcgacttt gacgcgggtc agctgatcca cggtaaagcg atgccgcagt tgctggaaga
     9181 atttatcgac accatcgttg agtttgccaa cggtaaacaa acctgcaacg agcgtaacga
     9241 cttccgcgaa ctggcgatct tcaaaagcgg cgtaacgcta taaagtcgaa atgagttgtt
     9301 aataaaaaac ggcgtttcat aatgtgaagc gccgtttgct tttccatacc tgataaggat
     9361 ttatcatgac cgcgtattgg ctggcccagg gcgtgggtgt catcgccttt ctgattggta
     9421 tcacaacatt tttcaatcgt gacgaacgtc gcttcaaaaa gcagctttcg gtctatagcg
     9481 ccgttattgg cgtacatttt tttcttctgg gcacctatcc cgctggtgcc agtgccatcc
     9541 ttaatgccat tcgtacattg attaccttac gcacgcgcag cttatgggta atggcgattt
     9601 ttattgtgct gactggcgga attggcctcg cgaagttcca tcatcctgtc gaactattgc
     9661 cggttatcgg tacgattgtc agtacctggg cgctgttccg ctgtaaaggg ctcaccatgc
     9721 gctgcgtgat gtggttttca acgtgttgct gggtgattca caacttctgg gcggggtcga
     9781 taggcggcac aatgattgag ggtagttttc tgcttatgaa tggcttgaat atcattcgtt
     9841 tctggcggat gcagaaaagg ggaattgatc cgtttaaagt agagaaaacc cctcccgccg
     9901 tagacgaaag gggttaaaca attaattacg cagggcgcta tttgccagac gatcgtcttc
     9961 cgcctggcaa gctgccgcag tgaacagcac gtcagttgaa gagttcagcg cggtttcgca
    10021 agagtcctgc aatacgccga tgataaagcc gacggcaacc acctgcatgg cgatatcgtt
    10081 cgaaataccg aacatattac aggccagcgg gatcagcagc agagaacccc ccgccacgcc
    10141 ggatgcgcca caggcacaca gagaagccac cacgctcaac agcagcgccg tgggcagatc
    10201 gaccgggata cccagcgtat taaccgcagc cagcgtcaac acggtaatgg tgattgctgc
    10261 gcccgccata ttgatggtgg cacccagcgg aatagaaacg gaataggtat cgcgatccag
    10321 attcagcttt tcacacagtg ccatattcac cggaatgttc gccgcagagc tgcgggtgaa
    10381 gaaggcatac acgccgcttt cgcgcaggca cagcagcacc agcgggaacg ggttacgacg
    10441 aattttccac cacaccagca atgggttaac caccagcgcc accagtaaca tacagccaac
    10501 cagcacgacc agcagttgcg cgtagcccca cagtgtggag aaaccggtgg ttgccagggt
    10561 agaagaaacc agaccaaaaa taccaatcgg tgcgaagcga atgaccagtt tcaccataaa
    10621 ggtgacggca ttcgacatat cgttgaccag gtttttggtg gtctcgttac cgtgacgcag
    10681 tgcgaaaccg aggccaattg cccacaccag gatcccgatg tagttacctt tcagcagcgc
    10741 gtcgatgggg ttggaaacca tgctcattac cagcccgcgc atcacttcga caatgcctga
    10801 cggcggcgaa atatcacccg cgctactgga taaatgcagg gtagaaggga aggcaaagct
    10861 gaagactact gcggccagag cagcagagaa ggtacccagc agatagagga acaaaatagg
    10921 gcggatattg gttttctgcc cgtgctggtg gttagcaata gatgccatca ccagcatcaa
    10981 caccaggatg ggggcaacgg ctttcagtgc gccgacgaac aaagtaccta acagaccaac
    11041 agcttccgcc gcgggttttg agatccatgc cagaagaatc cccagaacaa ggccgaccag
    11101 gatttgtttt accaggctgc catgagccag acgccggaat agccccggtg aacgttgcgt
    11161 agtcattttt cgatcctttc attgtgttgt cgcacatccc tggtgcgttc tgtcgacgca
    11221 tctttcagga tgtaacaaag tgtttgcaag gtcgagtata aggaaagtta cagcttcggg
    11281 aagcgaaaaa tgctggattt taaagagagg tcatattttg caacttaatg gttacattta
    11341 tttgacataa aaataaagcc aacgtgcggc ttccagactg atgacaacat caaaaaagga
    11401 ctcggtcagt cccctcgccc catttgggga gagggttagg gtgaggggaa caggccagca
    11461 tcggtgcgaa aaattaccct caccccgacc ctctccctgg aagggagagg gagaaaacgg
    11521 tgctcatgtt aatgacaggg tacacggctt aggcagatta aaaattatcc accccgctgc
    11581 ttatcatgcc gataattcac ccaggcgttg ataataaacg tcatcaccag aatgccaaac
    11641 accacgccca gcgagacggc gattggaata tggtagaagt cgacaatcag catcttgata
    11701 ccgataaaca ccagaatcac cgccaggcca tatttgagca tcgagaaacg ctctgctacg
    11761 cccgccagca ggaaatacat cgcacgcagg ccgaggattg caaacaggtt tgaggtcagc
    11821 acaataaacg gatcagtggt cacggcgaag atagccggaa tgctatccac ggcaaaaatc
    11881 acgtcgctca attccaccag aatcagtacc agcatcagcg gtgtggcgta cagcaatcca
    11941 ttcttacgca caaagaaatg ctcgttgtcg atggtgtcgg tcatgcgcaa atgaccgcgt
    12001 agccagcgca ccagcggctt gtcgccaatg cctgattcat cttcatgggc gagcgccatc
    12061 ttcacgccgg taaacagcag gaaggcaccg aagatataca ggatccagtc gaactgtgaa
    12121 atcagccagc tgcctgtgaa gatcatgatg gtacgcagaa caatcgcccc gagcacgcca
    12181 tacaccagca cgcggcgttg taatgccgcc ggaacagaga aatagctgaa caacatcagc
    12241 cagacaaaga cgttatcgac cgccagcgat ttctcaatca gatagcccgt gagaaaggcc
    12301 agtgcctgtg gatcggcaac ggcgcgacct tcagtttgca ccagatacca ccagaaagcg
    12361 gcgttaaaca gtaacgacag cgtcacccag accagcgacc aggccgcagc ctgtttcatg
    12421 gtcatggcat gtgccccacg acgcccctgc aacaacaggt cgatagccag cataatggcg
    12481 acaacaacag cgaatccgcc ccatagcaac ggcgtgccga cagtattcat agaagttcct
    12541 tacacataaa aaaacggcca acgtcggaag acgtcagccg ctgcttttta tgcatagacc
    12601 tcgccttccg gcaaggtctc acttacaaca aaaaaggaat acgtctgcgt attcctttcg
    12661 ggttgcccgg tgaccggatg tggtttttca cacatcgtaa tgacgatcga ccggcaatga
    12721 agttactccc ctttgcaggt aacaaagtat gacagaccat tcagccagtc aatggtctgt
    12781 tacatacctt ttacactgtt ttactttggc aattatagtt ttacgctatc tgccggaaaa
    12841 atcaccccag tctggcggcg gatctcggtc agcagtttgg cggtgatgcg actgaccgcc
    12901 agccccggat gatccaccag atgctcatcc accagggtag cgaacagctc tgcttcgtag
    12961 agcatggtat taatatgctg cggctgggtg agatcctgca tttggctgcc acgcggcacg
    13021 aagcacactt tctggcattc agacagtttt tcaatcacca gcgatcctgc ttcgccctga
    13081 atctcgctcg ccaggacaga atcactgact tttgagtgct gcaaggtgac gctgaaatca
    13141 ccgtaatcca tcaccaccac gccgtgggca tctacaccgc ttgccagcaa actggcggtt
    13201 gcctgcacgc ttttcggctc accaaataac gccaccgccg acgccaggca gtaaaagccg
    13261 atatccataa ttgaaccgtt agagaatgac ggattaaagg tgttgggatt ttcaccgtcc
    13321 aggtaacgtt gatagcgcga ggaatactgg caatagttga aaaagacttt acgcaatttg
    13381 ccgactttcg gcagcgcctg gcgcagcaaa tgaaagttcg gcaggcaggc ggttttaaat
    13441 gcctcaaaca gcaccacctg attttcccgc gcacaggcaa tggcggcatc cacttccgcc
    13501 agattcgacg ccagtggttt ctcgcaaatc acatgaattt tatggctaag gaaaagttgt
    13561 gtctgggaaa agtgcagggc attcgggctg gcaatataca ccgcgtcaat ggcatcgctt
    13621 tccgccatcg cttccagcga ggtaaacaga tgctcgacag aaaaatcatt ggcgaagtgc
    13681 tgagcctgct caaggctgcg ggaatatacg gcggttaact tgtatttacc gctctcatgg
    13741 gcggcctcga cgaactggcg agtgatccag ttcgtaccaa tcacagcgaa acgtatcata
    13801 acgcgtggat actccataaa gggattctgt cgtcaatgta gcacgccttt ttcatcttcg
    13861 acgtgcgcag atacgcatta ttcttttgcc ggacagccat gctcactaaa cggcccaatc
    13921 atgaccgatg gcccgagata tttcggctgg cgatggagga ttttggcgtc aatcacggcg
    13981 ctgacacgag cgagtttctc ccgcagttga gtataaaaac cgtgcttcat attgaggtca
    14041 ggcgcgttaa aaccgatagc gtcgatgccg tactgttttg ccagccagat ggcgcgttga
    14101 ttatgaaatt cttgcgagat aatggtgata tggttttcac caaaaacctt tttggcacgc
    14161 actaccgaat ccagcgttga gaatccggca tagtcgcaga agatcacttt cgccggtacg
    14221 cctttagcga tcaaagcctg ctgcatacct gaggcttcat cgtaattttt acgtccgtta
    14281 tcgccactta ccagtagcca tttcactttt ccggcgtggt acaacgctgc tgcggtatca
    14341 atacgtcggg taaagtagcg attacccggc ctggccccca gcaataagcc cacgttacgc
    14401 gccggaactg cgttgacatc gctccaggtc agctgcttgc tggcgttgac cataacgcga
    14461 tcggcaataa aaatggtcgc tcctgctata agcaacaaca aacagccaat cttaagagtc
    14521 cggcgagaaa aacaaatacg gagaagaagg cggggctgca tggtcgttat ccttaacgaa
    14581 aggggggagg tcgattttag gcgctaaaga aagaaaagat aacgcctgac atcattttca
    14641 gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga actcaagctg
    14701 gtggtactgc ggctccatat ggcagcacaa ctggtaaaac gctttgttat gctctttctc
    14761 cttcaggtgc gccagttcgt gcaccacgat catacgtaaa aacggttccg gcgcgttgcg
    14821 aaacacagta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct gcacacgaga
    14881 aacagcggta tgtaacccga gcgcgttttt tagcacatgg atcttattgt catacatcac
    14941 tttattgatc ggcggggcat tacgcagaaa ctgatttttc agatcctgag tatattgcca
    15001 gagggcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca gcacatcgcc
    15061 cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt agctgctcgg gataaccctg
    15121 aagataagta agattgttca taaccgccca cgaaaaagag aaaaagggta tactcacgca
    15181 cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag ccacttagac
    15241 aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt taacccgcta
    15301 caggcgtggg aagcggcgga tgaatatttg ctgcaacagt tggacgacac agaaatccgc
    15361 ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct ggcggaacat
    15421 aagccgtaca gcattggcga ctcatacatc agtgaactgg cgacgcgcga gaatttacgc
    15481 ctcaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga ctacccgcaa
    15541 cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga acagcaactg
    15601 cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc caaagcccgt
    15661 gacattcaca cttccacgct ggagctgttc gagaaagtgc tcggcccgac caccactacg
    15721 ctggcatgga aaaaagcgcg cctgattaat tgcactttca atgaaccgcc gctggccgat
    15781 gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca caaccatgcg
    15841 aatgtcttct cccgcaccgg gctggatatt ggcgcacgct tctttatgca acatctgcca
    15901 gagaatctcg aaggtgagat tgtcgatctc ggctgtggta acggcgttat tggcctgacg
    15961 ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg atgaatcgcc gatggcggtc
    16021 gcttccagcc gaatgaatgt tgaaaccaac atgccagagg cgttggatcg ctgcgagttt
    16081 atgatcaaca acgcgctctc cggagtggag cctttccgct ttaatgctgt gctctgcaac
    16141 ccgccgtttc atcagcaaca tgcgctgacc gataacgtcg cctgggagat gttccaccac
    16201 gcccgccgct gcctgaaaat caacggcgaa ctgtatatcg ttgccaaccg ccatctggat
    16261 tacttccata aactgaagaa gattttcggc aactgcacca ctattgcgac gaataataaa
    16321 tttgtggtgc tgaaagcagt gaagctgggg cgtcgtcggt aagattggca gcaacctgcc
    16381 ggatgcggcg taaacgcctt atccagccta cgttcagcac gttaccccca ggccggataa
    16441 gacgcggcaa gcgtcgcatc cggcaatggc tgctcatttc tggaatcgcg tttccataaa
    16501 acaaaaaatc cctgtcacca tcctctacat tctcttgttt agagtttttc tacgtttatg
    16561 ctacatacgt gaagttatga cgcacaaaga atatgatttc tttaccgccg ttcatcgcac
    16621 taaggggaaa aaatgattgc gctttccaac atcttgcagg caggagaaca gctaaccgct
    16681 gtggcacctt ttctggcggg tattcagaac gaggaacaat acacccaggc gctggaactg
    16741 gtagatcatc tgctgctcaa cgatcctgaa aaccccttgc tggatctggt gtgtgccaaa
    16801 ataaccgcgt gggaggaatc agcgcctgaa ttcgcagaat ttaatgcgat ggttgaagcc
    16861 atgcctggcg gtatagccgt gattcgtacc cttatggatc aatatggttt aaccctttcc
    16921 gatctgccgg aaattggcag taaatctatg gtgtcacgcg tttcgagcgg gaagaggaaa
    16981 ttaacgctgg aacacgctaa aaaattggca acgcgattcg gcatttctcc cgccttgttt
    17041 attgattaac gcgacgcgcc ggatgcgttg catccggcgc ttcaaataac gatttaaatc
    17101 tccagcgcca gccgtgttcc ctgggcaatt gcccgtcgtg cgtccagctc catagccaca
    17161 tcgcagccgc caattaaatg cacagttttc ccgctatcaa tcagcggttg cgccagcgcg
    17221 cggtttggct cttgccctgc gcagatcacc acattgtcca ccgcgaatac ctgtgtttcg
    17281 ccgttgatca ccacatgcag cccgtcatcg tcgatcttct ggtaactgac gcttgggatc
    17341 attttcacac cgcgcgagag cagggtagtg cgatggatcc agccggtggt tttgcctaac
    17401 ccctgtcctg gtttgctggc tttgcgctgg agcatcacaa tctgccgtgg gctacgaggg
    17461 atctgcattc cctgcgggct taagccacca gcctgttgca ggctactgtc gatcccccat
    17521 tcattacaga attcggcgat gttctggctg gtggactccc ctggctgact caaatacatc
    17581 gccgtatcaa aaccaatccc gccacaaccg atgatggcca cctttttacc caccggcgct
    17641 ttgtcgcgca atacatcgag ataactcaat accttcggat gatcgatccc gtcgatgggc
    17701 ggaatgcgcg gcacgatccc gctggcaagg atcgtttcat cgaacgactg taactgatcc
    17761 gccgtcacgg tgtgattgag tttcagcgtc acccccgtca cttcgatcat ccggcggtaa
    17821 tagcgcagcg tttcgtaaaa ttcctctttg ccggggatct gtttggcgat attaaattgc
    17881 ccgccgatct cactgtgggc atcaaacaac gttacctgat gcccacgcgc cgccgcgtta
    17941 atggcaaacg ccagcccagc aggtcccgca ccgaccaccg ccagattttt tttctgcacg
    18001 gcaggaagga ttggcatttt ggtttcgtgg caggcgcgag gattcaccag gcacgaggtg
    18061 actttgccaa cgaagatctg atcgaggcag gcctgattgc agccaatgca ggtgttgatc
    18121 tcatcggctc gccccgattg cgcttttgac agcagttccg catcagcaag aaacggtcgt
    18181 gccatcgaca ccatatcggc atcgccgcgc gagagaatgt cgtcggcaac ctgcggatcg
    18241 ttaatccggt tggtggtcac cagcggcagc gaaacgtggc ctttcagttt gcgcgtgacc
    18301 caagtaaatg cgccgcgcgg cacgggcgtg gcaatggtcg gaatacgtgc ttcatgccag
    18361 ccaatgccgg tgttgataat ggtcgcgccc gccgcttcaa tggcctgcgc cagctctacc
    18421 gtttcggcaa aagtcccgcc gtcttcgacc aggtcgagca tcgacagtcg gtagataata
    18481 atgaagtcgt tgccgacgcg ttcgcgcacc gcacgcacta cttctacggc aaatcgcatc
    18541 cggttgcggt aatcgccgcc ccactggtca ctacgctgat tggtgcgcag cgtcagaaat
    18601 tcgttgatca aatacccttc ggaccccatt acctctacac cgtcgtatcc tgcctcccgt
    18661 gccagttgcg cgcagtgggc gaaatcgtcg atcagttgca ggatctcttc atgggtgagt
    18721 tcgtgaggaa cgaaacggtt gatgggggcc tgcaatgcgg agggagcgac cagatgcggt
    18781 tggtagctgt agcgcccggt atgcaaaatt tgcagggcta ttttgccgcc ttcctgatgt
    18841 accgcttcgg taatggtgcg atggtgtggg atctggctgg cgtcgttgag cattgcgcca
    18901 ccttccatgc caacgcctgt taaatctggt gcaatgccgc cgctgacaat cagcgccacg
    18961 ccgtgacggg cgcgttcggc ataaaacgct gccagccgct cggcaccgtc cgggtattcc
    19021 tccagcccgg tgtgcattga gcccatcaac acgcggtttt ttaacgtggt aaaacctaaa
    19081 tccagcgggg cgaacagcga cgggtagctc ataaaattgt ccagtatgta aaataattgt
    19141 tatgtggtcg gatgagttct aatttagccg tcgccggaga aaagggaaaa ggggagtgcg
    19201 gggtttgtga tgggattcaa aaagaatacg cccggtggta acgccggatg cgacgcttgc
    19261 cgcgttttat ccggcctggg ggattgcaca tgtagggcgg ataaggcgtt cacgccgcat
    19321 ccgccagtgg cacggtgcag atgccggatg cgacgcttgt cgcgttttat ccggccagga
    19381 ggattgcaca tgtagggcgg ataaggcgtt tacgccgcat ccgccagccg ttgcaaatta
    19441 ttgcttacgg aaaaaatcgt tatacaacat atacaaatgc gcggcactcc aggagaaatt
    19501 tggtgcgcct tgctgtgcgc cagtcagcgg gttgtaattc tcctgaattg ggccatcggc
    19561 ggttaaccct ttggcgtgcc ggaagaacgt atccgccagt ttcagggcat catcgcgata
    19621 accgtaacgc tccatccctt tcagaccaaa ccagaactga tccacccata cgcgcccgcg
    19681 ccagtagata tcagcgccaa aggccggatt ggttaacgcc gccgttccca gcggaacaaa
    19741 ggtattgaac tctttaggat cgagcatcac cttcaccacc gcgtcggcat tggcctgcgt
    19801 tgccgcaccg ttaaacagcg gcgaccagcc ttccggccct ttaccgcgct caacaatcgg
    19861 tttgcccgcg cagccgttcg ccagcggttt atcttcaata cgcacgtcat agaagtactg
    19921 cgtagccggg tcgaacatac aggtgttgat gtagtccgcg agctgctgtg ccaactggcg
    19981 atagcgctta gcctcttccg gtttaccgag aatcgtcgcc atctccgcca gataatggtt
    20041 atcgctgtac atatagctgg cctgatccac cgactcctgc aatagcgagt accccagcaa
    20101 cgttccgtcc tgactgcggt tttcggcgaa tttcaccgtc cagtcgctac gtttgccgcc
    20161 gcttgcaaca tatttatcca gctgttcttt gtcgataaac ccaaagacgg cggcatcatc
    20221 acgccccgat tcccacgatg cagcaacctg tgccggaatt tccagactgt cgtactggcc
    20281 tttctccacc acgcgggcgt agttgttcag cccagactgc gtctcttctt tgtcgctttt
    20341 cttcaccata aacagcatct cgccgctctc agtgttgtgg gctttgtcgc gggtcgcgcc
    20401 atattccggc acgccgttgc cgttgtgatc gcggttacgt aaccaccagt cgtgataggc
    20461 caccagtttc gggtacatct ctgccaccca ggttttatcc tgggtgacgt tgtacacttc
    20521 catcaccgac caggcggcaa ggctgggttt ggtatttcgt tcgttccagt tgccaccatc
    20581 gccaccacgc tcggggctaa gattccacgc tatcaggtcg gggacaaagc ccacatcctg
    20641 cggacgcacg ctgtcgccag gctggatctg ccaggagaag accgcgcgga tattctcttt
    20701 ggcgatgtcc ggattgaaat gcgccatcgc aaacgcctgc ttccaggtat cccacggcca
    20761 ggtctgattg ccggagaacc agcgcccggt caccgacggt gtaacggtgt taaatttcac
    20821 cgcaccgccc ggtgagcgcc agttaccgtt gagcgtttcg atcgctttca ccgcgacgcg
    20881 cgtctgttcc ggcgtcgcat ccggattggt taaccctttc ttcaggtatt cttcccagcg
    20941 ttgctgcgag gcggtgagat aaaacgccgg acgcgccaga atatcgcgga tctgcatttg
    21001 ctctttgcta acttcctgag cggtcagcag atgggaatag gtggtgtaga gcgtggtcga
    21061 accgttgata tgcgccttac tggtaaagcg attaccgttg atttcagtct gcaccggcag
    21121 ggatttatgc acctgatatt ccgattcgcc ggaggtcagc agatcccagg tggcgcgcac
    21181 tttgccaaag gtgactttca ggccatcacg ggtggcgctg attttgcgct gatagtcagg
    21241 gtattcgcca gcaatggttt tatcggaaag cggtttccct tctttcgctt ccagtttttc
    21301 cagcagttcg ccgtcccaca ccagatccag cggtttattg ctggtgattt tggtttccag
    21361 tagtgacgtg cgcggcgtgg cgaagcgcag agtcatttcg acttgcacat cttttgctgt
    21421 cagtttttgc accagcgcac cgggaatact gtatgcctcc agcgtaaagt cgactttctt
    21481 gccgtcctgc cagacggtca ggcggtcgaa attgctggcc ataaagttga tgtactcttc
    21541 cgtcagcagc gcaacgcccg gaaagccgcc catggtgtta gggccgtctg gcaacagatg
    21601 accatgccag gctccgagat cgaaaaacgg attaaaacgc tggtgatcgt cgtaatcgta
    21661 atccttcatg tactgcggcg cgccagtacg gttaatcacg tttttataat tgtcggcgtt
    21721 agcggcatgg gcggaaaaac ttatcagcag agcgcaggtt actggcgtta aaatagtttt
    21781 tattttcatg aaattacagt ccttattacc agtagaaata ttgcatcagg aagatttggt
    21841 cgctgggatc gtgagaggca tttttcatgt agaagcggct atcaaaagcc gtggcgaaac
    21901 gtccgccgcc gtattcgtac cagataccaa actgcatgaa agaggtggtt tcatcgtcag
    21961 cgtggtcagg gcgatgcttg gcgtaggtgt aagcggtgga gagataaacg ccctttaacg
    22021 cttcatacat ggcgctcacc atcatcccac tttcacttcc cggtacgtcc agcccgtcgg
    22081 cgcgtccggt atggtgccag gcgcgagcgg caaagttagg agttagcagg cccgtgagat
    22141 agagctgtcc tgtgccgtcg gtgatttcca gaccgttcat ccaggtgatg ccttcggcaa
    22201 ggtcatactg gatgtaaccg ttcatcattg ccggataggt gtatttgtcg tcatagcgat
    22261 cgtacttgcc aaaatgtagc caggctttgc tttcgtcatg atgtccggca ggtgtggcgg
    22321 tgacgctata acgaaagttg ccgcttaagt tctgcacctt cagcgcggtc ataatgtcgc
    22381 gggtattggg aatgacgtag cccagatcgg gggtgaagtc gccccaccat tgcaggtcat
    22441 ccagcgagga atcctgacgc agactgagca tgatttcggt gccatcattg gtacgataac
    22501 cgccatagaa acggttaatt ccgccctcga aaccacccca gctatggtct ggcacccagg
    22561 cattgccgtc gtggtcggcc tgcacggtcc agccttcgcc gtaaatcagg ccgaaccagg
    22621 agccgtactt catctccagg ccaccttcaa tataggtacc gtcgctgtat ttatgtttat
    22681 tctcaccctc taaatccata tatccaccaa cccctaattc gccgtaaaaa cgaaatgaag
    22741 gagaagatgt cgaggtaatt gcaggtgttt ttgtttcgtc atgttctgcc ggacgtgctt
    22801 cttctcctgc cgcagaaaag gcgtagcaaa acggcagagc aagtaatgct ctgcatggtg
    22861 cagtgattaa tttcattgtg ttaatccatg agctaaagaa aaagtacagc cttttatcgt
    22921 tgtaattata aaaatataaa ggcagaaact attttccctc tcctcatcca ggaagaggga
    22981 aaatgcctgc tcatttttta ttattctgct taatcaacat tgctggctgg cgtcgcttca
    23041 atatggcgct cttcagccgt taatcccttt atatatttac tgtatttcca ccacgcaaag
    23101 ccaaggaaga taacaatacc gccgacgtta taaaaaatga tggtcagaat attcgcgcca
    23161 gtcgggaatg tcgaagcgac aaaccctacg gcaaatatcg caatcagcat tgaaacaacg
    23221 ataataccgg tgcggcggga gcccatacgg aaatcgcgtg gcaggtgatc taatttggcg
    23281 cgtaaattca ggtaagccag catgatgaat aacggcggaa gcatggacgc tgcggcggtc
    23341 atattaataa tagtattcat cagatcctgc acggtattgg aaccgagcat cgggataatc
    23401 atcagcggga tgacgatcag gaactggatc cacgctgcgc gcgccggaac gccgttttcg
    23461 ttcagttcga cggttttctt accaaagatg ccttccggga tttcggagaa gaaaattttc
    23521 accggcgttg cggtccacat cagcagggaa ccgaacatcg cggtaaagga caccagaccg
    23581 acaaagcgat tcatcaacgc ttccggtaga ccaaaatacg ccgccatgcc gtggaatacc
    23641 tgcaccgatc cgccggtaaa tttcaactct ttgctgctga cgaagacgtt aatcagcacc
    23701 gaggagacgg aatacagtac gccgataaaa atcccggcga ggatgatcac tttaacgaac
    23761 gatttcgaac cgcctttgac gtcgttaacg tacacagcga cggactccgc gccacctgcg
    23821 gccataaaga tccaggtggt aacgccgagg aacgcccagt tgaagttcgg gatcatcgcg
    23881 tcaacggtga tggggtcagc aggctgtacg ccgccaacca gcgccgtacc tgccagtaaa
    23941 atgtaggaga gcgttaacag cagcatcagc gttgaagtga cggaggtaat tggccccaac
    24001 attttcgccc cgttggtgga aacccaggtg gagaaggcga acagcaccat actgataatg
    24061 gtggtggcaa ccggcgtcat aatatattcg tatccgagga aggcatacga agcataagca
    24121 ataacgcgcg gcaacagtga ggtgaaaaag aacaggttta cgaaccagta ggtataggca
    24181 gtaataaatg cccaacgtcc gcccagcgaa ctttttaccc acgcgtagac accggcttct
    24241 gagtttttat ttaacgaaac aaattctgcg atgatcagac agaagggaat aaaataaaaa
    24301 atcgtcgcga ggaaaaacat cggtgccgag gccagtccaa gctcaatatt attattgata
    24361 acgttgttaa agctgtaaac ggcggcaaaa gtcagcgaga gcaagccgaa tttgccgatt
    24421 gtattacgtt tggtatcaga cataacacat ctccgaaaat gtgtagggta gcggtggcgg
    24481 caaactcctt ttgccgccgt agttgttatt tgttatggaa ataaccatct tcgatggtga
    24541 ctttgagaac cacttttttg accgcgttat tgcagataaa ccgatacgct tcatggatat
    24601 cgcaaataac gatttgccct tcgtggacct caacggtttc tccgcagcct tttaaatatt
    24661 cacggtcagt ttcatcacga taatattcca ctacctgtaa tgtctctttc ggtgcatatt
    24721 caattttttg ctgcccttgc aggtagtaat gcacttcaaa atagcgtcga tgcccggtaa
    24781 atagcgcatc ggtcgcggag tctgtctcca cgcggtaagt caatgagtca ccgatggagt
    24841 gggcgacgcc aggctgaatg ttgtcgatat tttcaatcgc ttcaacgcag cgttgccact
    24901 tcttgccaga ggcgtaaatc tggcggaact gttctaagtt atcgatgatc ctcattgctt
    24961 attctcgctg tgcaaattcg tggaaaagaa ccctgcgccg aactcatacg acgccaggct
    25021 ttgcgcggta gcttctccgc cagaaaccgg cagcaacgta aagccgtagc tgaagtcacg
    25081 gaaccagacg cgccaggagt ccagcacctc gctgccccag gagttggagc cgaggccaag
    25141 tagctggtgg tcgaggttca gggtgatgtc atcactgcgc tgcagctcgt tacagtgctg
    25201 ggcagcgtgg atgttttcct gggtatagtg ccaggcgctg aagttaattg ggcgctgcgg
    25261 aaccaccagc agaccgttgc cgtggcggtt agtcagtgcc gtccagcgga catgctgacg
    25321 gttgccgttg ttctgcggga agggatagtt ctcgaacatg gcatcgacgg tgctgcgcca
    25381 gatatcgatg atgttagcct gctggctgtc ggcgtagttt tcgcccggtc cacgaccgta
    25441 atacgccacc tgatcgtatt cgccgttaat tcccatggtg aaaccgatgc acggaatgat
    25501 gtgcggatag tcgccgtaac gctcgccgga aagcgccacg ttaacctggc catcggcagc
    25561 gatgcgccag atgtaggtgc agcgcatccc gaagtcaaac accggcgggg caataactgt
    25621 gcggctgatg atcagcactt caccatcgct ctgttctacg gcaaagtcgc gcagatgttc
    25681 ctgcatgatc tgcaaatgat tcggttgcca cagcccttcg tactcctgct tgtggttgtc
    25741 gatcatcggc ttgaagaagt tgatctttgg ctcgcgagtc agcagcgatt cgccattcac
    25801 ctgccaggat gtcggtttgc cactcatttt tgagaaggtg atcgcgaagt tgtagccgcg
    25861 aacggtgcag ctcaaacgat cgtcttccag cgtcagcgga cgcgcattat ttggtgcgaa
    25921 aggcactggc tgcgcggtgt tttccttcag cgggaactga taagtggcga tagaatgccc
    25981 ggcttcgctg tagcgggtgc gggaatcttt ggtcaccgta atgttgagga acgcttcgcg
    26041 ggcgtccagc tgcggcagcg tgatctgcaa gggggcttcg ctgttcggcg caacgtcgcg
    26101 cagtttaatc tgctgcgtcg cgagcgtttc accttcgacg cgcacctctg cgtgcagggt
    26161 gtagtcatca agcgtggtaa accacagttt attttcgact ttcagctcgc cgtgagtcag
    26221 atccagcgcg tggattttta ccggcgcgat aacctgtttg tactctttca ggcccggtcc
    26281 cggcgtctga tcggaataga tcaaaccatc aagacagaag ttatagttgt tgggatagtc
    26341 gccgtagtcg ccgccgaatt tataccagac gttgccgttg tcatcctgcg cctggatccc
    26401 gtggtcgcac cactcccaaa cataatgtcc ctgaatacaa tcgtgcttat agaagacgtt
    26461 ctggtactcc gtcagcccgc ctggtccgtt ccccatcgca tgagcatatt cacagatgat
    26521 gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc agcggcacgc gggtgtacat
    26581 ggtggaaata atatcgacca cttcagcatc gcgatcttct tcgtaatgca ccagtcgcgt
    26641 gtcatccagc gccttcgctg cgtggtacat cgcgcggatg ttacagccat agccggattc
    26701 attgcccagc gaccagatga tgatcgacgg atggtttttc tgcgcgtgaa tatggcgaac
    26761 aatgcgctcg acgtagactt tttcccactg cggatcgtcg gtaatacggc tgatatcgcc
    26821 gacattagca aagccgtgcg attcgacgtc ggtttccgcc atcacaaaca agccgtagat
    26881 atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga gcggtacgca cggagttgat
    26941 attgtgctgc ttcatcaact ggagatcttt ctcgacgcga tccattccaa cggcgcggcc
    27001 tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc atcacataac ggttattgat
    27061 ccagaacaga ccgtcgcgca ctttgatatc acggaagcca acgcgttgtg gcaccacttc
    27121 cagaacgttg ccgttggcgt ctttcagcgt catgaccaga tggtaaagat aaggggattc
    27181 tgctgaccat tgctgcggct gttcgacagt aaaagcaaag ctggcgctgg tcagtttttc
    27241 aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt tcgccatcaa acagggtata
    27301 ttccagcgtc gtgacgacag gggaggcggc gagattttcc agcaccactt cgcaggaaag
    27361 cgtggcatcg caataggctt cgtcaaagtc ggtacgcaca gtgaaatcgt taatatgcgt
    27421 taggtgtttt ccgaccagat aaacatcgcg gaagatcccc gctgaccacc acatatcctg
    27481 gtcttccacg taggtagagt ccgcccactg catcacgcgc acacacaaca ggttgtcgcc
    27541 ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg cgactgccct tgctgaaacc
    27601 cacatactga ccgttaacat agacttcaaa ataggtttcg acgccgtcaa atttaatcag
    27661 cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata cgttgatagg caccggttgg
    27721 gttatcgctg gggacaaacg gcacatcgat ggggaacgga aaaccttcgt cggtatattg
    27781 cagtttgccg tgaccttcca tttgccacat ggcggggacg gtaatatgcc cccagtcagc
    27841 cattaactca gaggtgaagg cttctggtac ttgcagcgga tggtcaaaaa agtggaaatt
    27901 ccactgaccg cttaagggca gaaacaggct gctggtttcg cgggcaaagg tacgcgcttg
    27961 cgcaacagaa tcatatgaaa aaaagtacgc acgcggcgca agtcggtttt cgtgggtgag
    28021 ctgaatgttt tcccagcgat tcatagggcc tcccggtaga gagaaatcgg aaccattcat
    28081 ggtgatggta gtttgtgtaa ttgctgggga gtttagtaaa atttttacta aaaagtagca
    28141 ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt ccggcaaaaa
    28201 cgattgccgg acagtgtgta aggggattta acgggtcgtg ccgcgcagtt ttaatttgct
    28261 gggaacgaag actaacagcg gcagcgcgcg accatcgcgg gctttttcat acaccaggtt
    28321 aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg gcggaaaggt
    28381 aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcctgtg ggatgttcag
    28441 gcctcgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag caacaaacag
    28501 tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac ccgacgaact
    28561 ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc ggccatattc
    28621 cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc caataaaacc
    28681 aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga tgcgtgccag
    28741 atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa aacagatatt
    28801 gtcggtcaac gcacaggcgg cggcgcgcag ggcgggcgtg ggtttgccga caattaaaat
    28861 accggtgacg tttttaatgt ctggtaatcc gctgtgttca taacagttgg tgagctcaat
    28921 gcccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt aaggatcgtt
    28981 gatctccagc tcctgttggt agctgtagat agccagaata tggtgttggt tgactgcacc
    29041 tgtctggagt ttacgggcac tactggtctt gtactccagc ttttcggcga tctcgagaat
    29101 gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga ccctggatac
    29161 tgtcgccagg gatacgccag cttcgattgc gatgtctttt agtgttgcca tgtttacctt
    29221 ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg tcacctgttt
    29281 tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct cacgaatgtg
    29341 atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt gctatagttt
    29401 agtaaaattt tacccgaagg gaggaaacaa tggaaaccgt ggcttacgct gattttgcac
    29461 gtctggaaat gcgcgtcgga aagattgtgg aagtgaaacg ccatgaaaac gccgacaagc
    29521 tgtacatcgt acaggttgat gtgggggaaa aaacactgca aaccgtgacc agcctggtgc
    29581 cgtactacag cgaagaagag ctgatgggga aaacggtggt ggtcttatgc aatctgcaaa
    29641 aagcgaaaat gcgtggtgaa acgtcggaat gcatgctgtt gtgtgcggaa accgatgacg
    29701 gcagtgaaag cgtgttgtta acgccggagc ggatgatgcc tgcgggcgta cgcgtcgtat
    29761 aaaaagatcg gatggcgacg tcgtatcgcc atccgatttg atattacgct tcttcgacac
    29821 ttactcgcat ggccgccagc gccttacgcg ccgctttgat caccagttca cactgttcaa
    29881 tggtcagtgt cagtggcggt tcaatgcgga tcgttttggc gttattgagc gttccggcca
    29941 ccagtacgcg ctggcggaac atctcgctgg caaagttata gccgatttcg ttatcaacaa
    30001 actcaattgc catcaacatc cctttaccac gcgcttcctg taccagatcg ggatattccc
    30061 gcgccagttg acggaaaccg tccagcaaca tatcgccttt ttgctcagcc tgagccggta
    30121 agttctgctc cagcaacaca ttgatggtcg ccagcgccgc cgcacaggcc agcgggttgc
    30181 cgccaaaggt ggtggtatgc aggaatgggt tgtcgaacag aactgaaaac acctcttcag
    30241 tggcgatggt cgcgccaatc ggcatcacgc cgccgccgag cgctttggca aggcagagga
    30301 tatccggctg tacgttctca tgctcgcagg cgaacatctt gcccgtgcgc cccatgcccg
    30361 tttgtacttc atcgaggatc atcagtgcgc cgaactcatc gcatagctta cgtacggcgg
    30421 tgagatagcc cggcggcggc agaattacgc caccttcacc ctgaatcggt tcgaggatca
    30481 ccgcagccac atcatcaccg gtttttttgc actcgttaag agccgtgcgc atggcttcga
    30541 tattgccaaa cggcacatga cggaagcccg gcagcagtgg cataaacggt ttgcggaagg
    30601 tcgatttcgc cgtggccgac agcgcgccaa gtgatttacc gtggaacgcg ccgctggtgg
    30661 caataaaagt aaacttgccg cgcggtgact ggtaagcttt cgccagcttc agcgctgctt
    30721 caacggactc ggtgccgcta ttacagaaga agctgtattt cagtttaccg ggcgttagcg
    30781 cagcaagggt tttcgccaac atcgcccgta acggatcgag cagctcctgg ctgtgcagcg
    30841 gttgtttcgc aagttgattc tgtacggcgg aaaccacaac tggattacgg tgccccacgt
    30901 tgaaaattcc aaaacctccc aggcagtcga taaactcctg tccttgggtg tcgacaagcg
    30961 tatttaaacc tcccgcttgc cactctacgg ctccgtaatc cccgccggcg gtaacagatt
    31021 tgcgatactc taaaaacccc ggattgacat gctctttgaa gtattcaatc acctctcggt
    31081 taagtgcttt catctcctca tgatccagcg ttcgcttctc aatgagattc agggcgtggg
    31141 cgctgcacgc taaagccgat gcgctcgaag gtaacctgtt caaaatatgc tccggaggct
    31201 cgcgtatcac atgataccga tttaagtatt gcagggattg cgccactccg gctgcgatcg
    31261 cgaaaatcgg gataaacaca aattcggtta tcatttgtgc aatatttaat gctatctgtt
    31321 aacatttgtt gttattttgc acggtttttg tcgggtatgg atttgatgat tgctgcgcaa
    31381 atgcacgttt tcagggcaat aattgcctga tttttgggca agataattaa cgttttc
//
LOCUS       DAZFRN010000046        31309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFRN010000046 DAZFRN010000000
VERSION     DAZFRN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.046"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKUB_002012"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKUB_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO6655034.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHKUB_002013"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHKUB_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO6655035.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHKUB_002014"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHKUB_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO6655036.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKUB_002015"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKUB_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO6655037.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKUB_002016"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKUB_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO6655038.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKUB_002017"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKUB_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO6655039.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKUB_002018"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKUB_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO6655040.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKUB_002019"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKUB_002019"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO6655041.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKUB_002020"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKUB_002020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO6655042.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKUB_002021"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKUB_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO6655043.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKUB_002022"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKUB_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO6655044.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKUB_002023"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKUB_002023"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO6655045.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKUB_002024"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKUB_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO6655046.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKUB_002025"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKUB_002025"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO6655047.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKUB_002026"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKUB_002026"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO6655048.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEVDVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKUB_002027"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKUB_002027"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO6655049.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKUB_002028"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKUB_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO6655050.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKUB_002029"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKUB_002029"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO6655051.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKUB_002030"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKUB_002030"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO6655052.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKUB_002031"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKUB_002031"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO6655053.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKUB_002032"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKUB_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO6655054.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKUB_002033"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKUB_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO6655055.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKUB_002034"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKUB_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO6655056.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24797)
                     /gene="zraP"
                     /locus_tag="ACHKUB_002035"
     CDS             complement(24366..24797)
                     /gene="zraP"
                     /locus_tag="ACHKUB_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO6655057.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGMGYGGCGGGGHMGMGHW"
     gene            25035..26411
                     /gene="zraS"
                     /locus_tag="ACHKUB_002036"
     CDS             25035..26411
                     /gene="zraS"
                     /locus_tag="ACHKUB_002036"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO6655058.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26408..27733
                     /gene="zraR"
                     /locus_tag="ACHKUB_002037"
     CDS             26408..27733
                     /gene="zraR"
                     /locus_tag="ACHKUB_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO6655059.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGHFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27730..29019)
                     /gene="purD"
                     /locus_tag="ACHKUB_002038"
     CDS             complement(27730..29019)
                     /gene="purD"
                     /locus_tag="ACHKUB_002038"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO6655060.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29031..30620)
                     /gene="purH"
                     /locus_tag="ACHKUB_002039"
     CDS             complement(29031..30620)
                     /gene="purH"
                     /locus_tag="ACHKUB_002039"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO6655061.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            31235..>31309
                     /locus_tag="ACHKUB_002040"
     rRNA            31235..>31309
                     /locus_tag="ACHKUB_002040"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7419 a         8109 c         8539 g         7242 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gtatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaagggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt actcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtggggg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acagaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt gcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gaattgcgag gcatttagcg
    14881 cccgctctac gtcaacagcc gttttgccgt tgatacgcag gcggatgtca tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttcaacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg ctacggcaat
    15121 cgccgtattc actaacaccg cgtcggcccc catttccagg gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg aactgtggcc tgctggataa taatctccag
    15241 catggcacag gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcatttc ggcggctttc agagtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc caattttagc cagttagtgc ctaacgcttc
    15481 acgtgccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cagcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagtcgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcgac
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgccgagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacctcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggcgtga tgatgccctg gcgggcgtag tgcagttggg tgacacggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggcagtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta gtgcgtgaaa acgacggtcg
    21541 ccgcgtaccg gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    21661 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    21721 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agttgcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggc gctctgtttc tcactgcctg
    23761 tagtcacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctga tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccca ttccggctcc gcgcggaata cccgcttcag ccatcgcgat atctcgtttc
    24481 acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt
    24541 ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc
    24601 agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct
    24661 gtctgctgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga
    24721 gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt
    24781 ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt
    24841 ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat
    24901 gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta
    24961 cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc
    25021 aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa
    25081 gcgcgatcct ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc
    25141 gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac
    25201 ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc
    25261 agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca
    25321 cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt
    25381 attccccgca ggaaatgcag cagttacatc ctggagatga agaagcgtgg cggcggatcg
    25441 atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg
    25501 ctggaatgca ccggatgcgc catatgcagc agtatgccgc aacaccacaa gcaattttca
    25561 tcgctttcga cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga
    25621 ttatcctctt cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc
    25681 gccgctatct gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg
    25741 tggcgctggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaatcca ctttcctcaa
    25801 ttaaagggct ggcgaaatac tttgccgaac gcgcgccagc agggggagaa gcgcatcaat
    25861 tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg cgtggtaagc gagttgctgg
    25921 aactggttaa gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc
    25981 actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg
    26041 ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga
    26101 atctctatct caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggtca
    26161 gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag
    26221 atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc accggattgg
    26281 ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc
    26341 aggagggaaa aggctcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc
    26401 cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg
    26461 cactattttg caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg
    26521 gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt gcgatgtgcg
    26581 aatggcggag atggacggca tcgccacgct gaaagagatc aaagcgttaa acccggcaat
    26641 tccggtgctg attatgactg cgtactccag cgtcgagacg gcggtagagg cactgaaaac
    26701 tggggcgctg gattatctca tcaagccgct ggatttcgat aacctacagg cgacgctgga
    26761 aaaggcgctc gcgcatacgc acagtattga tgctgaaaca cctgcggtga ctgccagcca
    26821 gttcggtatg gtcggtaaaa gctcggcgat gcaacacctg ctcagtgaaa tcgccctcgt
    26881 cgcgccatcg gaagccacgg tactgatcca cggcgattcc ggcaccggta aagagctggt
    26941 cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg
    27001 tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt
    27061 tactggagcc gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgctgtt
    27121 tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctgc gtgcgattca
    27181 ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc tcggttgatg tccggctgat
    27241 tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcattttc gccaggatct
    27301 ctactatcgc ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga
    27361 tatccctctg ctggctggcc attttcttca gcgctttgcc gagcgtaatc gcaaggcggt
    27421 aaaaggcttt acgccacagg cgatggattt gctgatccac tacgactggc cgggaaatat
    27481 tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg aatatatttc
    27541 cgaacgcgag ctgccgctgg cgattgccgg tacgccgatc ccgctggggc aaagtcagga
    27601 tattcagccg ctggtagaag tcgaaaaaga agtgattctg gcggcactgg agaaaacggg
    27661 cggcaacaaa accgaggccg cccgtcagtt agggatcacg cgcaaaacgc tattggcaaa
    27721 actgtcgcgt tagttctgct cgcgttcgat agcgcgccag ccaatatctt tccggcagaa
    27781 gcagtcgtcc cagtgaatat cggtcattaa ggcataagcg cgtttctgcg cttctgccac
    27841 ggtatgaccc agcgcggtga cgcacagtac gcgcccgccg ctggtcacta cctgctcgtt
    27901 atccgccagt ttcgtgcccg cgtggaacac tttgccgtca gccacttctt ccagcggcag
    27961 gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac
    28021 gccgagagaa gcgcgttcat cccactcgga tgttttctcg tccagcttcc cttcacaggc
    28081 tgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg
    28141 atcgccaaag cggcagttaa attcgataac cttcggattg ccctgtttgt cgatcatcag
    28201 gcccgcgtag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg
    28261 ccagatgata cgttccatgg tgcgctgatg aacttcatcg gttactaccg gcgcggggga
    28321 gtaagcgccc atcccgcccg tgtttggtcc ggtatcttta tcgcctacgc gtttgtgatc
    28381 ctggctggta gccatcggca acacatgctc gccgtccacc atcacgataa agctcgcttc
    28441 ttcgccatcg aggaactctt cgataacgat gcgatgaccc gcgtcgccaa aagcgttgcc
    28501 cgccagcata tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc
    28561 tttcccggca gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata
    28621 cgccagcgca ggttctacct cggtgaagtt ctggtattcc gccgtaggga tcttatggcg
    28681 ggccaggaaa tctttggtaa acgcttttga gccttccagt tgggccgcac ctgcggttgg
    28741 gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc
    28801 ttccgggccg acgatggtca gatcaatctt ttcgttttgt gcgaaatcca acagcgccgg
    28861 gatatcggtc acgccaatag caacgttttg cagcgcgggt tccagcgcag tgcctgcatt
    28921 acccggagca acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc
    28981 gtgctcgcgc ccgccgttac caatcactaa tactttcatc tattgctcca ttaatggcgg
    29041 aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact
    29101 tcgtcatcac ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg
    29161 gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact
    29221 tccaggcctt catcagcagc tttaataccg gcgattttcg cggagtacac gcggctcatc
    29281 tggcccgcgc caataccgat ggtcatattg tttttggcat agacgatagc attggatttc
    29341 acgaacttcg ccactttcca gcagaacagc gcatcgcgca gttcctgttc ggtcggctga
    29401 cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg atcctgaacc
    29461 agcagaccgc cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg
    29521 caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg
    29581 gaaggcgcaa taatcacttc gacaaactga cgagaaatga tggcctgtgc ggtttccgca
    29641 tccagctcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac
    29701 gcgcgatcgt aagcatcaag aatggaattg ccgatagcta caccgcaagg gttggcgtgc
    29761 ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta
    29821 tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag
    29881 gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa
    29941 cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accagcggct
    30001 tctttgcttt caccgtggta cgccggaacc atgctgccga agtagttggc aatcatgctg
    30061 tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc
    30121 agcgatcctt cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc
    30181 acgattgcga catctttatg gttcttagcg gcggagcgca ccatggtcgg gccgccgata
    30241 tcgatgttct caaccgcatc ttccagcgag caaccttcac gggccacggt ctgggcgaac
    30301 ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc cataatggca
    30361 tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg
    30421 cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct
    30481 ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa
    30541 agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg
    30601 actggacgac gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc
    30661 tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg
    30721 cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca
    30781 attttgacag tggttaccgt gggaaaaata cagaaattac attgatgatt gtggataact
    30841 ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt
    30901 ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg
    30961 cggatgtgaa tcacttcaca caaacagccg gttcggttga agagaaaaat cctgaaattc
    31021 agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gcgctaaagc
    31081 gcgtcgcaac tgctctttaa caatttatca gacaatctgt gtgggcactc gaagatacgg
    31141 attcttaacg tcgcaagacg aaaaatgaat accaagtctc aagagtgaac acgtaattca
    31201 ttacgaagtt taattctttg agcatcaaac ttttaaattg aagagtttga tcatggctca
    31261 gattgaacgc tggcggcagg cctaacacat gcaagtcgaa cggtaacag
//
LOCUS       DAZFRN010000047        30252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFRN010000047 DAZFRN010000000
VERSION     DAZFRN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.047"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            124..1707
                     /gene="yegH"
                     /locus_tag="ACHKUB_002041"
     CDS             124..1707
                     /gene="yegH"
                     /locus_tag="ACHKUB_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6655062.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLSKDGEMEYEV"
     gene            complement(1981..3834)
                     /gene="asmA"
                     /locus_tag="ACHKUB_002042"
     CDS             complement(1981..3834)
                     /gene="asmA"
                     /locus_tag="ACHKUB_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO6655063.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3856..4437)
                     /gene="dcd"
                     /locus_tag="ACHKUB_002043"
     CDS             complement(3856..4437)
                     /gene="dcd"
                     /locus_tag="ACHKUB_002043"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO6655064.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4529..5170)
                     /gene="udk"
                     /locus_tag="ACHKUB_002044"
     CDS             complement(4529..5170)
                     /gene="udk"
                     /locus_tag="ACHKUB_002044"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO6655065.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5488..8805
                     /gene="dgcE"
                     /locus_tag="ACHKUB_002045"
     CDS             5488..8805
                     /gene="dgcE"
                     /locus_tag="ACHKUB_002045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6655066.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNVWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8844..9701)
                     /gene="alkA"
                     /locus_tag="ACHKUB_002046"
     CDS             complement(8844..9701)
                     /gene="alkA"
                     /locus_tag="ACHKUB_002046"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO6655067.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9835..11187
                     /gene="yegD"
                     /locus_tag="ACHKUB_002047"
     CDS             9835..11187
                     /gene="yegD"
                     /locus_tag="ACHKUB_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6655068.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11199..13139)
                     /gene="yegI"
                     /locus_tag="ACHKUB_002048"
     CDS             complement(11199..13139)
                     /gene="yegI"
                     /locus_tag="ACHKUB_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO6655069.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13136..13897)
                     /gene="pphC"
                     /locus_tag="ACHKUB_002049"
     CDS             complement(13136..13897)
                     /gene="pphC"
                     /locus_tag="ACHKUB_002049"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO6655070.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13894..14553)
                     /gene="yegL"
                     /locus_tag="ACHKUB_002050"
     CDS             complement(13894..14553)
                     /gene="yegL"
                     /locus_tag="ACHKUB_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6655071.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14774..14833)
                     /gene="ibsA"
                     /locus_tag="ACHKUB_002051"
     CDS             complement(14774..14833)
                     /gene="ibsA"
                     /locus_tag="ACHKUB_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO6655072.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15106..15162)
                     /locus_tag="ACHKUB_002052"
     CDS             complement(15106..15162)
                     /locus_tag="ACHKUB_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6655073.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15434..15490)
                     /locus_tag="ACHKUB_002053"
     CDS             complement(15434..15490)
                     /locus_tag="ACHKUB_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6655074.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15769..17010
                     /gene="mdtA"
                     /locus_tag="ACHKUB_002054"
     CDS             15769..17010
                     /gene="mdtA"
                     /locus_tag="ACHKUB_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO6655075.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            17010..20132
                     /gene="mdtB"
                     /locus_tag="ACHKUB_002055"
     CDS             17010..20132
                     /gene="mdtB"
                     /locus_tag="ACHKUB_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO6655076.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVNDTQFELMMAIALVVMIIYLFLRNIPATIIPGFAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPELMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20133..23210
                     /gene="mdtC"
                     /locus_tag="ACHKUB_002056"
     CDS             20133..23210
                     /gene="mdtC"
                     /locus_tag="ACHKUB_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO6655077.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23211..24626
                     /gene="mdtD"
                     /locus_tag="ACHKUB_002057"
     CDS             23211..24626
                     /gene="mdtD"
                     /locus_tag="ACHKUB_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6655078.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQAVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24623..26026
                     /gene="baeS"
                     /locus_tag="ACHKUB_002058"
     CDS             24623..26026
                     /gene="baeS"
                     /locus_tag="ACHKUB_002058"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO6655079.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAGQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26023..26745
                     /gene="baeR"
                     /locus_tag="ACHKUB_002059"
     CDS             26023..26745
                     /gene="baeR"
                     /locus_tag="ACHKUB_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO6655080.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26936..27268
                     /gene="yegP"
                     /locus_tag="ACHKUB_002060"
     CDS             26936..27268
                     /gene="yegP"
                     /locus_tag="ACHKUB_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO6655081.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27477..27773
                     /locus_tag="ACHKUB_002061"
     CDS             27477..27773
                     /locus_tag="ACHKUB_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO6655082.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27775..28071
                     /locus_tag="ACHKUB_002062"
     CDS             27775..28071
                     /locus_tag="ACHKUB_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6655083.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28174..29535
                     /gene="yegQ"
                     /locus_tag="ACHKUB_002063"
     CDS             28174..29535
                     /gene="yegQ"
                     /locus_tag="ACHKUB_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO6655084.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29866..30183)
                     /gene="yegR"
                     /locus_tag="ACHKUB_002064"
     CDS             complement(29866..30183)
                     /gene="yegR"
                     /locus_tag="ACHKUB_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO6655085.1"
                     /translation="MKKIAAISLISIFIVSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         7001 a         8053 c         7986 g         7212 t
ORIGIN      
        1 atgtgaggtt attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg
       61 ccccgattta tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt
      121 actatggaat ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc
      181 gaactggtcc tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg
      241 ccgaagcagc gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg
      301 ttactgctgg cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc
      361 tcgtttacct ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc
      421 aaagccacga tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa
      481 cgcaaaggtg cgaagttctg gggagtggtg acacaaattg tggtactgga cgccatcttc
      541 tcactcgact cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc
      601 gccgtggtta tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt
      661 aacagtcacc cgacgatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc
      721 ctggtggcag aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt
      781 ttctcggtga tgatcgaggc gctcaatcag ttggctatct ttaaccggcg acgttttctt
      841 tccgctaacc agacgctgcg ccagcggact accgaagcgg taatgcgcct gcttagcggg
      901 caaaaagaag atgcggaact ggacgccgaa accgcgtcca tgctggtgga tcatggtaac
      961 cagcagatct ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag
     1021 cgtaccgtca gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg
     1081 ccggaagacg agatccgcca gttactggag cgaaatcagc atacgcggct ggtggttacc
     1141 gacggtgatg atgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag
     1201 tcactgcgcg gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg
     1261 gaaaccttgc cgttgttacc tgccctggag cagttccgta atgcccgcac gcactttgct
     1321 tttgtggtgg atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa
     1381 accattgccg gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag
     1441 aagaatgcgg acggttcctg gacggctaat ggtcatatgc cgctggagga tctggtgcaa
     1501 tatgtgccgc tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg
     1561 gaatatttac agcgtattcc aaaacctggc gaagaagttc aggtagggga ttatttgctt
     1621 aaaacgttgc aggtagaaag ccatcgcgtg cagaaggtgc agattatacc gctgagtaag
     1681 gatggcgaga tggagtacga ggtgtgatgg tatcggccat tttacccgat agccgaaagg
     1741 aataagatga gggaaccgcg ccagcggcga tttaccgccg ggagcccggg ttgccagggt
     1801 ggtggcgatt gagccaccct ggcacgttca ccggcttgag cgttccatag tagtgaggaa
     1861 catcaagtga acggaacagc cttcgatctt acatattcag aatataatgg tgccgccacg
     1921 aacgcctgat gcgacgcttt acgcgtctta tcaggcctac aggacaggtc acctggtaac
     1981 ctacatcttc tccagcaact tcttcacatc tttgccattg cgggaatctt tattccgctc
     2041 ggcccagtca ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc
     2101 cacttgcaga ctgtaattga gttgctgcca gttgccataa acccgcagcg gtactggcgt
     2161 ttctttgagg aaatcaatca gtttgctttc cccgttccag ccacccacga cccgaatatc
     2221 aaactgggtg tcgcaggttt gatctgccag attcaacatg ccttcccctg tcagcgccag
     2281 cacaggcgat tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc
     2341 ggtggtaaag cggtcaagac gcgttacgtt atcgaagttt tcagcggcct tcacatcacc
     2401 accattacgt tctaccgctt gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt
     2461 gtcggtcatt tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt cggcatctat
     2521 gtcagcaccg gagaaatcac cagccagtga catttttccg gtcaacgaaa tcggatagtt
     2581 aaacgccttc agaatggtac caatctcaac gttttccagc cgtggctgga agtttatccg
     2641 cggatttatt gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac cgttaagctt
     2701 gccctgcagt tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc
     2761 tgtaaaattc attccgcgcc agcgcacgtt actggcctgc aacaaaatat cagccgtaaa
     2821 gccttgcagt ccctgatatg ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa
     2881 tgttgattgg ctctgcccct gctgcgcggc accgttttca ccattcgctg tttcattaag
     2941 cgggatgagg ttgtcaagat tcagttgcgg aaattgcagt ctcagctgcc attcaggttt
     3001 ctctgtcagc gtgacctgtg cttgcccgct cagcgtgctg tcattggcgg tcaagctgat
     3061 ttggttaaat gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac tcccctgccc
     3121 ctgaatacct tgttttggta aatctgcacc ctgcaactgc cagttaattt gttcaatagc
     3181 cgccgttaaa tcatgcggat aatcagaagc atctaccgta ccgttaaggg atatcgtgag
     3241 atcgcgctga tcgcgattaa cccgcccgga gaactcaaat gaaccacgat gttggggatc
     3301 ttgttccatt tgcaggcgaa tattgcggat tgtcacctgc tcgtcatctt catgctggaa
     3361 caccagcacg ctgtccgcca ccttaagact ggatatatca aacgaccatc cgcgatcgtc
     3421 tgacagatcc ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc
     3481 ttccgtctgc ggcgtcagtt ggatcactgc cccttttagc atcacctgct taacgctcag
     3541 ttgatgactc agtagtggta aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag
     3601 tggctggctt gcaccctggg cggtgagaga cattcgcccg gagaggatac taagctgcgg
     3661 ccagacgtgc caacgcagtg gcccgtcgag ctgcaattga taaccgctac gtgcagcaac
     3721 ttgcttgacc atatagtcgc ggaaatcatt cggattcacc agcaacacta acgcagataa
     3781 cccggccacc agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc
     3841 ctcaatgggc ctgaattagt ctttatcgat tcggctggct accgcgccct gctggttgcg
     3901 atatttcgca tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa
     3961 gctcagcgca ccaattaaca tgcccggacg cagcgccagc ggcagcttac cggagttgta
     4021 gaactccagc acaatgcaac cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac
     4081 catcagcccc agacgcgcca aagaggaacg cccgtccagc cagcccacca gatcggctgg
     4141 cagcgtcacc gactccagcg tcaccgccag cgccagctct cctgggtgaa gatagaacgc
     4201 ctcgccctcg tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc
     4261 atctttggga ccgctcagat cgataaacgc tgccgtgtga ccacggaagg tacgaaattt
     4321 attgcccagg cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt
     4381 gatcgacaaa cggccttcat caagccaggc ttcaatatct cggtcacaca gacgcatggc
     4441 attttctcct ttcgcgcatc actcccttaa cgccaattac gtcaagggca taccaggtta
     4501 tcactgaacg gtacacaatt tatcaagctt attcaaagaa ctgacttatt ttcgctttca
     4561 atatatcgat cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc gcatattgtt
     4621 tagaaggctc aatgaattgc aggaacatcg ggcgcacggt tttttgatat tgcgccatca
     4681 cggaatccat tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga
     4741 tatccagcgg ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg
     4801 tcagcaacaa aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc accgtttctt
     4861 tcatacgcgt atgttcaaca tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca
     4921 acgcttgtaa atgctcaagc aacaggctgt gatccatcgc gctgggatgg tcgtagttgg
     4981 tcttaacgcg ttcttccatc gacagatggc tttgatcttt gtaatagcag tcttcgggaa
     5041 ttacgccgat gtgttcatca ccgacttgct cacgtaattc acgataaagg gtactggcaa
     5101 taagactctt gccggaagcc gatgcgccag cgataccgat aatgacgcac tgatgagact
     5161 gatcagtcat atatttagcg acctgattaa cctggatgtt aggaaggggg cgacgaagcg
     5221 ccaaacgcgg caattatagg gatttcatcc gcctgatacc agtcgaatag cgttgccgcg
     5281 cgctcagagt taattgttga cgaagaattc ccggtggcaa attacgttga tcagttttat
     5341 acaaggtaaa aaatgttata cgcagttgcg caaattatcc gcctttacgt cactttatga
     5401 gcaattcgca tataaaatgt aaaacttttg tactagcata aacacagaaa cgaatactgg
     5461 cgacctggtc ttgcggataa agcggtaatg agcaaacaat cacagcatgt attaattgcc
     5521 ctgccccacc cgctgcttca cctggtcagt ttaggtttag tctcgtttat ctttaccctt
     5581 ttctcgcttg agctttcgca gtttggcacc caactcgccc cactgtggtt cccgacgtcc
     5641 atcatgatgg tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc
     5701 tgctcgctgg gaaatatcgc cgcatccatc ctgctttttt ccaccagctc gctgaacatg
     5761 acctggacga ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg
     5821 ctgccgtggt ataacccctt gcaaaatctg gctgactggc tgcgtctggc attcggcagc
     5881 gccattgttc cgcctctgct agggggtgtt ctggttgtcc tgctgacgcc cggagacgat
     5941 cctctcaggg catttttgat atgggtactg tcagaatcca tcggcgctct ggcactggtg
     6001 ccgctgggat tgttatttaa accacactat ctgctgcgcc atcgcaaccc acggttgctt
     6061 tttgagtcgc tgctcacgtt agccatcaca ctgacgttaa gctggctttc gatgctgtat
     6121 ctgccgtggc cttttacttt cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca
     6181 cgaatggaag cctttttgat cttccttacc acggtgatga tggtgtcgct gatgatggcc
     6241 gcggatccct ccctgcttgc tacgccgcgt acatacctga tgagccatat gccgtggcta
     6301 ccgtttttgc tgatcctgct gcccgccaac atcatgacga tggtgatgta tgcctttcgt
     6361 gcggaacgca aacacatttc cgaaagcgaa acccgttttc ggaacgccat ggaatattcc
     6421 gctatcggta tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc
     6481 tgccagtttc tcgggtacag tcaggaagag ctgcgcggac tcacctttca gcaactgacc
     6541 tggccggagg atctcaataa agatctccaa caggttgaaa agctgataag cggtgaaata
     6601 aacacctatt caatggaaaa acgctactac aaccgcaatg gcgatgttgt ctgggcgttg
     6661 cttgccgtct cactggtgcg ccacacggat ggcacgccgc tctattttat cgctcagatt
     6721 gaagacatta acgagctaaa acgcaccgaa caggtgaatc agcaactgat ggagcgcatc
     6781 acgctggcta acgaagcggg cgggattggc atctgggagt gggagttgaa gccgaatatt
     6841 tttagctggg ataagcggat gttcgagctg tatgaaattc ctccacatat caaaccgaac
     6901 tggcaggtgt ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt
     6961 cgtgattcgt tgcaatcacg ctcgcccttt aaactggaat ttcgcattac cgtaaaagac
     7021 ggtattcgcc atatccgcgc cctcgccaac cgggtactga ataaagaagg cgaagtcgaa
     7081 cgcctgctcg gcattaatat ggatatgact gaggttaaac agcttaacga ggcattgttt
     7141 caggaaaaag agcgcctgca cataacgctg gactccatcg gcgaagccgt ggtctgtatt
     7201 gatatggcaa tgaaaattac ctttatgaat ccagtggcgg agaagatgag cggctggacg
     7261 caggaagaag cattaggtgt tccgctcctg acggtgttgc atattacctt tggcgacaat
     7321 ggtcccctga tggagaacat ttacagtgcc gacacctcac gttccgcgat tgaacaagat
     7381 gtggtgttgc actgccggag cggcggcagc tacgacgtgc attacagtat tacgccgtta
     7441 agtactctgg acggcagcaa tattggttcg gttctggtga ttcaggacgt taccgaatca
     7501 cgcaaaatgc tgcgccagct gagctacagc gcctcccatg atgcactgac gcatctcgcc
     7561 aatcgcgcca gttttgagaa gcaactgcgt atgctgctgc aaacggtaaa cagtacacat
     7621 cagcgacatg ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc
     7681 gggcatgcgg cgggcgacgc tttgctgcgc gaactggcgt cgttgatgct gagtatgctg
     7741 cgttccagcg acgtgctggc gcgactcggt ggggatgaat ttggtctgct gctaccagac
     7801 tgtaatgttg aaagcgcgcg ttttatcgct acacgcatta tcagtgccgt gaatgactat
     7861 cactttatat gggaaggccg tgtgcatcgg gtaggtgcca gtgccgggat taccttgatt
     7921 gatgacaaca atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc
     7981 tccaaaaatg gtggacgggg ccgggtgacg gtttacgaac cacagcaagc tgccgcacat
     8041 agcgaacggg cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga gaatcagttg
     8101 atgatgatgg cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa tgtgtggctg
     8161 atttcgctta aactctggag ttgcgaaggc gagattattg atgaacaaaa atttcgtcgt
     8221 agcttcagcg atccggcact tagccatgct cttgaccgac gggtattcca cgaatttttc
     8281 cagctggccg caaaagcggt tgccagtaaa ggcataagca tcgccctccc cctttccgtt
     8341 gccggtttga gtagcgccac gctggtgaat gatctgcttg agcagctgga aaatagccct
     8401 ctaccaccac ggttattaca tctgattatt ccggcagacg cgattttaga tcacgcagaa
     8461 agcgtgcaaa aactgcggct ggcgggatgt cggatagtgc tcagccaggt gggccgcgat
     8521 ctgcaaatct tcaactcgct gaaagcgaat atggcagatt acctgctact tgatggtgag
     8581 ttatgcgcca acgtgcaggg taatttgatg gatgagatgc tgattacgat tattcagggg
     8641 cacgctcagc gactcgggat gaaaaccatc gccgggccag tcgttttacc cttagtgatg
     8701 gatacgcttt ctggcatcgg cgtcgatctg atttatggtg atgtgattgc cgatgcccaa
     8761 ccgctggatt tgctggtgaa tagcagttat ttcgcgatta actgagggag cgtttgccca
     8821 ttgcctgatg cgacgcatca ggcctacaat tcgtccgttc cgtctggttg ccagccttcc
     8881 gtataccaga tatgcaacag cgcataagaa cgccagggct tccagcgctc ggcataacgg
     8941 cggatttgtg ccggtgtcat ccccggaaat cgctgtttaa tcagataatc atccggcaga
     9001 aaaacatctt tcgcctgcca gccacgcaaa gcgaaataat tcgccgtcca gcgcccgata
     9061 cccggaaaag tttgcagcgt tttcatcgcc tgctccacat cgcccggaat tgtcattggt
     9121 aagctgccct ccagcgccgc atttgccaga tgaatcagtg cctctgcccg tttcaacggc
     9181 atgcctaacg cttttaatgc ctgcgggtcg gctgctgcca gccgctgagg cgtggggaag
     9241 cagatatact ccggaaaatc atccagccgt tcgccataaa gctgtacaac tttggcagtc
     9301 aattttgccg ccatcgccac gctcaccagt tggcctaaaa tcgcccgcac accctgctca
     9361 aaagcatcaa tacagccggg taaacgcaat ccgggccgcg ccgcgcctaa cttgcccaac
     9421 gcaccgttaa caatctgcgg gttacattgc agatcaaaca ggcggctcat tttcgccaga
     9481 cactcagcgg caacgggttc taaacctgca cttaaattta tgtgcagagt atggcgggct
     9541 atatccggaa tagcagtcac cacgccgcga tattcgccca ccgccagact acgggcataa
     9601 taactgtccg cgaccgtttc cacaccactc actgcacggg cggcgagaaa tcccaacatc
     9661 cacgaccagt catacggcgg ctgccagttc agggtataca tcgcatctcc ttattcatcc
     9721 gctttcagca taaacgttat tcagacgcta cgctttgctt tcatattccg gttgtcgcga
     9781 cagcaacatt tcgctaaagt cacgcccctt cttcaccggc atggggatta attcgtgttt
     9841 attggttttg attacggtac agcaaactgt tcagtggcgg tcatgcgtga cggtaaaccg
     9901 caattactga aaatggaaaa cgacagcacg ctgctgcctt caatgctttg cgcgccaacg
     9961 cgtgaatcgg taagcgaatg gctgtaccgc catcatgatg ttccggcaga cgacgatgaa
    10021 acgcaggcgc tgttacgtcg ggcgattcgt tataaccgcg aagaagatat cgatgttacg
    10081 gcgaaaagcg tgcagttcgg tctttcctca ctggcacagt acattgatga tccagaagaa
    10141 gtgtggtttg tgaaatcacc aaaatcgttc ctcggtgcca gcggcttaaa accgcagcag
    10201 gtagcgctgt ttgaggatct ggtctgcgca atgatgttgc acattcgcca gcaggcgcag
    10261 gcgcagctgc cagaagcgat tactcaggcg gtgattggtc gcccgatcaa cttccagggg
    10321 ctgggcggcg atgaagcaaa cgcccaggcg caagggattc tggaacgcgc ggcgaagcgt
    10381 gccggattca gggacgtggt attccagtac gagccggtcg cggctgggct ggattacgaa
    10441 gccaccttgc aggaagaaaa acgggtgctg gtggtggata tcggcggtgg tacgactgac
    10501 tgttcattgc tgctgatggg gccgcagtgg cgttcgcgtc tcgatcgtga agccagcctg
    10561 ctgggtcaca gtggttgccg tattggcggt aacgatctgg acatcgcact ggcgtttaaa
    10621 aacctgatgc cgttactggg catgggtggc gaaaccgaaa aaggcatcgc cctgccgatt
    10681 ctgccgtggt ggaatgcggt tgccatcaac gacgtacctg cgcagagtga tttctacagt
    10741 agtgccaacg gtcgtctgct taacgatctg gtacgcgatg cccgcgaacc ggaaaaagtg
    10801 gccctgctac agaaagtctg gcgtcagcgt ttaagctatc gcctggtgcg tagcgcagaa
    10861 gagtgcaaaa ttgctctttc aagcgtagcg gaaacccgcg cctcactgcc gtttatcagc
    10921 gatgaactgg ctacgctgat tagccagcaa gggctggaaa gcgccctcag ccagccactg
    10981 gcgcggattc tggaacaggt gcaactggcc ctggataacg cccaggaaaa accggacgtt
    11041 atctacctga ccggcggtag cgcccgttcg ccgctgatta aaaaagcgct ggcagaacag
    11101 ttgccgggaa ttccgattgc aggcggcgat gactttggct ccgtcaccgc cgggctggca
    11161 cgctgggcgg aagtggtgtt tcgttaaaag aaaatggctc agcagcggct taaatctgcc
    11221 tgcgcctgag ccagtttttc tgccgcctga cgtaaaggtt ccatcaacgg catggtccgt
    11281 gctggagcat gaaggcggaa tcgctgcaac tctgtcgcgc cgacagtcaa cgtcgattcc
    11341 agccgatgtc gtttggcggt cattttcgcc attaccgctt gtctgtctgc tggcgttatc
    11401 gcttcattcg gcctgaacac aaaacggcgt tcacagctcg ctttccagtc gataaccgcc
    11461 tgggtcagat ggtcgccaaa ccctttcact tgcttaacgc cacgacgggt aacatctgcg
    11521 gctgtttcaa taccaaaaga ccgtaacgcc gctttacgcg cagggccaac gccgggaata
    11581 gaggcaacat caataaaaaa tccctccaga aacttctgct tctgccgttc ccttgcagtg
    11641 tcatgaagtg ctgccagagc gcgtttctct tcttccggta acccgagaat ttcgtccttc
    11701 attttttcga gcatcgcccg tttggcgata aaaccttcca gcccgcccaa ctgttggatc
    11761 tggctgacta aatgctcata gtccattttc gcgcgattaa atgcctcacg gcgttgctgg
    11821 atttctgctt tgtacgcttt gcttgtcaga ctgccaataa tccagatagc cgacagcaca
    11881 ggaaccaaaa taatataacg cggttctgcc tgaaggccgc aaagcaacag cgataacgct
    11941 gacagtgcga tctcaatcag aatgatgtat tcgcgccgta acaggcctaa aggaagcggc
    12001 ctgccagtcg gttggaaatg atcgggtaat ggcaattgca atgctggcgg tgctactgac
    12061 gccatcacca tcgcccagac tttcgccagc acaaaatcac cgccggtggt aatgacctct
    12121 tcgccgagat caataaaata gataacgcct tgattatcca gcgcacacca cgggcagtcg
    12181 gtcaaatgag cgggataaac atgcattgcc gaaacgggac atttctttaa ctgttggcgc
    12241 agagaatcaa gtgccgctac ccacgcttta gccgtcggac gcccggttgc cacgccactt
    12301 tccgtgaatg cctgctgaaa catggcttca acatcactcg gtaacatcga tagcggaatc
    12361 gatcgtggcg gcggttttaa accacgtcgc tgattatctg acgcgtaggc ataacggaaa
    12421 tgggtaatat ccgtctccag cgcattaccc gcatcagaga taagcggcac accagaataa
    12481 ggatgccggc caccaaacaa gacgtgaaaa atcagcaacg caaggccaaa attatcgtga
    12541 ttttctgtgc gttcaaagcc gacaaatgac gacaaggttt gtagctctgg cggcgtaaaa
    12601 tgcgacacgc cgacttcgca taaatgcagt gtgccattgg cgttaatctg aaaggagtcg
    12661 ctatcaatca gtaccacttt gctgtcgcga cctaccataa aactgttctg gtttacgtca
    12721 cccacgacgt gcccgtgctc gtgaaccgta gcaaaagatg aagcaatatt gcgcgcaaca
    12781 tagagtagaa aatcccacgc acaatgaggg taacgttgac gacgatgtgc cgggctatag
    12841 atcatatgaa tcggttcttt accagaaact tttggcatca taaaaccgat aacttttccg
    12901 cctcgtccac cgtgaagcgt tgcctgcggc caggcgacat aattcaacaa ttgcgcgtca
    12961 gctgtcgcag ccataaaggc aagtttgtcc tgttttaagg cgggtggcgg cgtgtgataa
    13021 atcttggcga cgctatcgac aaactcattg atatcataaa ccgcgccttc accgcctttg
    13081 cccagttcac ggcctggcgt aacgcactca ccagtagcag tatataaagt gggtttcatg
    13141 gtaaccacag cgccagtgcc aacgttttat catcatccgt acgctcgttt accgccgggc
    13201 tggacaaaaa ctgctttaac aattcaggta ataaatcgag ttgttcctgc gttgctgacg
    13261 ccagtccatt gaaaaacggg gtaaagaaag gtacatgagg agaattatcc agcatattta
    13321 acgccagtcg ctggatacca tccgtaaatg ctgcaacttt atgcacacgc tcagtgctgg
    13381 tgaaagtttc cagtctggaa acggcatctt catcggtaat gaagtgcgtc atattggcat
    13441 attcacccac catcggcgta agcggcaatt gcagaccgtg accaaaatcc accaccacgc
    13501 cgccatcgcc aatctgcata atcaaggtgc cattagccga tgaaatcaat cccagaaacg
    13561 tgcaggcaaa atcgcgaact gctaattctt tggcctctgc ctcggcaaaa agtcgctgac
    13621 gaatagtcag cacaatatcc gtggcgagga tatcattaag ccccaattcc ccaccctgca
    13681 ctttttgcga catataagcc atcgcttcat tgacggcgag catcgcgcct tcgccaccct
    13741 gcgagacgct gcctgcacca tcagcaagaa acatcaccag taatggttgc tgatcgttta
    13801 accaggcaac ctgcatctgg caggcatcct gacaaggtaa atcagcgcta atgtgcgacg
    13861 ttccaacagc ggatgcataa accagacgcc agctcacact gacgtccagc cttttggcgc
    13921 ttccagcaca acttccgttc ccggcgtgga gcgggaaacc gaacgcagcg aactggataa
    13981 ccagctaaac agttcacgga attgtaatcc ttgcagaggc aaaggctgac gaacgctgat
    14041 ttgtgccagc gtcttcatat ccgccccctg aacaccgatg gaaaagaagg caaacttctt
    14101 atcttcttcc ccctgaaaca ctttgttggc ggcagcctgc cactcatcgg ttggagcgcc
    14161 atcggtgatc aggaaaatcc acgggcgata atacgaaata ccatttgcgc ggtactcacg
    14221 tttcctctct tcaaccatat ccagggcttt ggtaatggcg gcccccattg gcgtatcccc
    14281 ctgggcaaac aggatgggcg ggaaaaagtt agcggcgctg gtaaagggtt gttccacatg
    14341 caccgggccg aacgtcacaa tgccgagttc cactcttttt aaagccagcg gatcggcaag
    14401 cagttcatca cgaaaggtca ccaatccggc gttaagttca ttaataggtc tgccgctcat
    14461 cgagccagaa acatccagca gcagaatgca ggggcaacgt ggttccgggt tactggcaaa
    14521 atcgctggtg gcaaacgtga tttgttcgct cataagaaat cctttcttag agaattatcg
    14581 aggtccggta tcataccgac tgcaataatg aaataagagc aaacgaagaa actttcagaa
    14641 ttaggcccgg cggcgcagaa aattttgcta ttcgcctcgc aagaacggag agccgtcgat
    14701 tgacactaag tgcggagtga cataatttca ggagtgaggg ttagggagag gtttccccct
    14761 ccccctggtg ttcttagtaa gcctggaagc taatcactaa gagtatcacc agtatgatga
    14821 cgtgcttcat cataaccctt tccttattaa aagccctctt ctccgggaga ggctttcccg
    14881 tttcagcgtc ccgctgaaat catcggctta cctcctttcg ccctgaatgc agtctatcgc
    14941 taacgcgtga ataaaatagt ttcctctgtg attattgcgt aacgactcgc aaagaatatt
    15001 tgctggcagg atcgcagact acaaagcctg cggattgaca atcttatcgt gaaggcatac
    15061 tttcaggagt gagggtagag cggggtttcc cccgccctgg tagtcttagt aagcggggaa
    15121 gcttatgact aagagcacca cgatgatgag tagcttcatc atgacccttt ccttatttaa
    15181 ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc gctgaaatcg tcggcttacc
    15241 tcctttcgcc atgcacttag tctatcgctg acttgcagat aaaatagttt ccagtgtgat
    15301 tattgcgtaa cgtctcgcaa agaatttttg ctggcaggat cgcagacggc aaagcctgcg
    15361 gattgacaat cttatcgtga aggcatactt tcaggagtga gggtagagcg gggtttcccc
    15421 cgccctggta gtcttagtat gctgggtagc taatcactaa gagcaccacg atgatgagta
    15481 gcttcatcat gaccctttcc ttatttaagg ccccttcctc gggaggggct ttcccgtttc
    15541 agcatccggc tgaaatctgc ggcttacctc ctttcgccct gtatgcagtc tatcgctaac
    15601 gcgtagataa aatagtttcc tgtgttatta ctggatgcgt gctcgcaaat gtgcccgcca
    15661 ttcagacgat tcccgatagt gtttcataat tcctccattt ttctccctta ttggctggct
    15721 acactagtat cattccgcga aacgtttcag gaagagaaac tcttaacgat gaaaggcagt
    15781 tataaatccc gttgggtaat cgtggtggtt atcgccgcca tcgccgcatt ctggttctgg
    15841 caaggccgca atgactcccg gagtgcagcc ccaggggcga cgaaacaagc gcagcaatcg
    15901 ccagcgggtg gtcgccgtgg tatgcgttcc ggcccattag ccccggttca ggcggcgacc
    15961 gccgtagaac aggcagttcc gcgttacctc accgggcttg gcaccattat cgccgctaat
    16021 actgttacgg tgcgcagccg cgtggacggt caactgatgg cgttacattt ccaggaaggc
    16081 cagcaggtca aagcaggcga tttactggca gaaattgacc ccagccagtt caaagttgca
    16141 ttagcacaaa cccagggcca actggcaaaa gataaagcca cgcttgctaa cgcccgccgt
    16201 gatctggcgc gttatcagca actggcaaaa accaatctcg tctcccgtca ggagctggat
    16261 gcccaacagg cgctggtcag tgaaaccgaa ggcaccatta aggctgatga agcaagcgtc
    16321 gccagcgcgc agttgcaact cgactggagc cgcatcaccg caccagtcga tggtcgcgtt
    16381 ggtctcaagc aggttgatgt tggtaaccaa atctccagtg gtgataccac cggaattgtg
    16441 gtgatcaccc agacgcatcc tatcgattta gtctttaccc tgccggaaag cgatatcgct
    16501 actgtagtgc aggcgcaaaa agccggaaaa ccgctggtgg tagaagcctg ggatcgcacc
    16561 aattcgaaga aattaagtga aggcacgctg ttaagcctcg ataaccaaat cgatgccaca
    16621 accggtacga ttaaagtgaa agcacgcttt aataatcagg atgatgcgct gtttcccaat
    16681 cagtttgtta acgcgcgcat gttagtcgac accgaacaaa acgccgtagt gatcccaaca
    16741 gccgccctgc aaatgggcaa tgaaggccat tttgtctggg tgctgaatag cgaaaacaag
    16801 gtcagcaaac atctggtgac gccgggcatt caggacagtc agaaagtggt gatccgtgca
    16861 ggtatttctg cgggcgatcg cgtggtgaca gacggcattg atcgcctgac cgaaggggcg
    16921 aaagtggaag tggtggaagc ccagagcgcc accactccgg aagagaaagc caccagccgc
    16981 gaatacgcga aaaaaggagc acgctcctga tgcaggtgtt acccccgagc agcacaggcg
    17041 gcccgtcgcg cctttttatt atgcgtcctg tcgccaccac gctgctgatg gtggcgatct
    17101 tactcgccgg gattatcggt tatcgcgccc tgcccgtttc ggcgctgccg gaagtggact
    17161 atccgaccat tcaggtggtc acgctctacc caggtgccag cccggatgtc atgacctctg
    17221 ccgttaccgc gccgctagaa cgccagttcg ggcagatgtc tggcctgaaa cagatgtcgt
    17281 cgcaaagttc cggcggtgcg tcagttatca ctttgcagtt ccagctaaca ttaccgctcg
    17341 atgtcgccga gcaggaagtg caggccgcga ttaacgctgc gaccaacttg ttgccgagcg
    17401 atctgcctaa cccgccggtt tacagcaaag tgaacccggc agatccgccg atcatgacgc
    17461 tcgccgtcac ctcaaccgcc atgccgatga cgcaagtgga agatatggtg gaaacccgcg
    17521 tcgcgcagaa aatctcgcag atttccggtg tcggactggt gacgctttcc ggcggtcagc
    17581 gtccggctgt tcgcgtcaaa cttaacgctc aggcgattgc cgccctcggc ctgaccagcg
    17641 aaaccgtgcg caccgccatt accggcgcta acgttaactc ggcaaaaggt agcctcgacg
    17701 gcccttcccg tgcggttacg ctttccgcta acgaccagat gcaatccgcc gaagagtatc
    17761 gccagctaat catcgcctac cagaacggcg cgccgattcg tcttggcgat gtcgcaaccg
    17821 tagagcaagg tgcagaaaac agctggcttg gcgcatgggc gaacaaagaa caggccattg
    17881 tgatgaatgt tcagcgccag cccggtgcta acattatctc caccgccgac agcatccggc
    17941 agatgctgcc acagctcacc gagagtctgc cgaaatcggt gaaggtaaca gtgctttccg
    18001 atcgcaccac caatatccgc gcatcggtca acgataccca gtttgaattg atgatggcta
    18061 tcgcgctggt agtcatgatt atctacctgt ttttgcgcaa tattccggcg accatcattc
    18121 ccggcttcgc agtgccgctg tcgttaatcg gcaccttcgc ggtcatggtg tttctcgatt
    18181 tttcaatcaa taacctgacg ctgatggcgt taactatcgc caccgggttc gtggtcgatg
    18241 acgccatcgt ggtgatcgaa aatatttccc gctatatcga aaaaggcgaa aaaccgttgg
    18301 cggcggcgct caagggcgca ggtgaaatcg gctttaccat tatctcgctg accttctcgc
    18361 tgattgcggt gttgatccca ctgctgttta tgggcgatat cgtcgggcga ctgttccgcg
    18421 aatttgctat taccctggcg gtagcgattt tgatctcagc ggtggtgtcg ctgaccctga
    18481 caccgatgat gtgcgcgcgg atgctcagcc aggagtcgtt gcgtaaacag aaccgcttct
    18541 cccgtgcctc ggaaaaaatg ttcgacagga taatcgccgc ctatggtcgt ggactggcga
    18601 aagtgctgaa tcatccgtgg ctgaccttaa gcgtggcact cagcacgctg ctgcttagcg
    18661 tgctgctgtg ggtgttcatt ccgaaaggtt tcttcccggt acaggacaac ggcattattc
    18721 agggcacttt gcaggcaccg caatccagct cctttgccaa tatggcccag cggcaacgcc
    18781 aggtcgcgga cgtgattttg caggatccgg cagtgcaaag tctgacctca tttgttggcg
    18841 ttgatggcac taacccgtcg ctgaacagtg cgcgtttgca gatcaacctc aaaccgctgg
    18901 atgaacgtga tgatcgggtg caaaaagtca tcgcccgtct gcaaacggcg gtggataaag
    18961 tgccgggcgt cgatctcttc ctgcaaccaa cgcaggacct gaccatcgat actcaggtca
    19021 gccgcaccca gtaccagttt accttgcagg ccacctcact ggatgcgctc agtacctggg
    19081 tgccagagtt gatggaaaaa ctccagcaac tgccacagct ttctgatgtc tccagcgact
    19141 ggcaggacaa agggctggtg gcgtatgtca atgttgatcg cgacagcgcc agccgtctgg
    19201 ggatcagcat ggcggatgtc gataacgccc tgtacaacgc gtttggtcag cggctgattt
    19261 ccactattta tactcaggct aaccagtatc gcgtggtgct ggaacacaac accgaaaata
    19321 ccccaggcct cgcggcgctg gataccattc gcctgaccag cagcgacggt ggcgtggtgc
    19381 cgctaagctc aattgccaaa attgagcagc gttttgcgcc gctctccatc aaccatctgg
    19441 atcagttccc ggtaacgacc atctccttta acgtgccgga taactattcg ctgggcgatg
    19501 cggtgcaggc gattatggac accgaaaaga cgctgaatct gccggtggat atcaccacgc
    19561 agttccaggg cagcaccctc gccttccagt cggcgctggg cagcactgtc tggctgattg
    19621 tcgcggcggt ggtggcgatg tatatcgtgc tcggcattct gtacgagagc tttattcacc
    19681 cgatcaccat tctctcgacg ctacccaccg caggggttgg cgcactgctg gcgttgctga
    19741 ttgctggtag cgaactggat gtgattgcga ttatcggcat tattttgctg atcggtatcg
    19801 tgaagaagaa cgccatcatg atgatcgact tcgcgctggc tgccgagcgc gagcaaggca
    19861 tgtcgccgcg cgaggcaatc taccaggctt gtctgttgcg ttttcgtccg atcctgatga
    19921 ccaccctggc ggctctgctt ggcgcgctgc cgctgatgtt gagtaccggg gtcggcgcgg
    19981 aactgcgtcg tccgttaggt atcggcatgg tcggcggcct gattgtcagc caggtgctga
    20041 cgctgttcac cacgccggtg atttatctgc tgttcgaccg cctggcattg tggaccaaaa
    20101 gccgctttgc ccgtcatgaa gaggaggcgt aagtgaagtt ttttgccctc ttcatttacc
    20161 gtccggtggc gacgatttta ctgtcggttg ccattaccct gtgcggcata ctgggcttcc
    20221 gtatgctgcc ggtcgccccg ctgccgcagg tcgattttcc ggtgattatg gtcagcgcct
    20281 cgctgcccgg tgcgtcacca gaaacaatgg cgtcttccgt tgccacgccg ctggagcgct
    20341 cacttgggcg cattgccgga gtcagtgaaa tgacctccag cagttcgctc ggcagcacgc
    20401 gtattatttt gcagtttgat tttgaccggg atatcaacgg cgcggcgcgc gatgtgcagg
    20461 cggcgatcaa cgctgcacaa agtttgctgc ccagcgggat gcccagccgt ccgacctatc
    20521 gcaaagctaa cccgtcggat gcgccaatta tgatcctcac gctgacatcc gatacttatt
    20581 cgcagggtga actgtacgat ttcgcctcga cgcagctggc tccgacgatt tcgcaaatcg
    20641 acggtgttgg tgatgtcgat gtcggcggca gctcactgcc cgccgtacgc gtcgggctga
    20701 atccgcaggc gctgtttaat cagggcgtct cgctggacga cgtacgcact gccatcagca
    20761 atgccaacgt gcgtaaaccg cagggggcgc tggaagatgg cactcaccgc tggcagatcc
    20821 agaccaatga tgagctaaaa accgccgccg aatatcagcc gctgattatt cattacaaca
    20881 acggcggcgc ggttcgtctg ggcgatgtgg cgacagtgac cgactctgta caggatgtac
    20941 gtaacgccgg gatgaccaat gccaaaccgg ctattttgct gatgatccgc aagctgccgg
    21001 aagccaatat tatccagacg gttgacagca tccgggcaaa attaccggag ttgcaggaaa
    21061 ccattccggc ggcgattgat ctgcaaattg ctcaggatcg ctcccccacc attcgcgcct
    21121 cgctggaaga agtcgagcaa acgctgatta tctcggtggc gctggtgatt ctggtggtgt
    21181 ttttattcct gcgctcgggt cgcgcaacta ttattcccgc cgttgcggtg ccggtttcgc
    21241 tgattggtac gtttgcggcg atgtacctgt gcggtttcag tctcaataat ctttcgttaa
    21301 tggcgctcac catcgctact ggtttcgtgg tggatgacgc catcgtggtg ctggaaaaca
    21361 ttgcccgtca tctggaagcg ggaatgaaac cgttgcaagc cgcactgcaa ggtactcgcg
    21421 aagtcggttt tacggtgctg tcgatgagtc tgtcactggt ggcggtgttc ctgccgctgc
    21481 tgttgatggg cggattgccg ggccgactgt tacgcgaatt tgccgtgacg ctttctgtcg
    21541 ccattggtat ttcgttgctg gtttctctga cattaacgcc aatgatgtgt ggttggatgc
    21601 tgaaagccag caagccgcgc gagcaaaagc gactgcgtgg ttttggtcgc atgttggtag
    21661 ccctgcaaca aggctacggc aagtcactga aatgggtgct caatcatacc cgtctggtgg
    21721 gcgtggtgct gcttggcacc attgcgctga atatctggct atatatctcg atcccgaaaa
    21781 ccttcttccc ggagcaggac actggcgtgt tgatgggcgg gattcaggcg gatcagagta
    21841 tttcgtttca ggcgatgcgc ggtaagttgc aggatttcat gaaaattatc cgtgacgatc
    21901 cggcggtgga taatgtcacc ggctttacag gcggttcgcg agtgaacagt gggatgatgt
    21961 ttatcaccct caagccacgc ggcgaacgca gcgaaacagc gcagcaaatt atcgaccgtc
    22021 tgcgggtgaa gctggcgaaa gaaccggggg cgaatctgtt cctgatggcg gtacaggata
    22081 ttcgcgttgg tgggcgtcag tcgaacgcca gctaccagta cacgttgtta tccgacgacc
    22141 tggcggcact gcgcgaatgg gagccgaaaa tccgcaaaaa actggcgacg ttgccggaac
    22201 tggcggacgt gaactccgat cagcaggata acggcgcgga gatgaatctg gtttacgacc
    22261 gtgacaccat ggcacggctg ggaatagacg tacaagccgc caacagtctg ttaaataacg
    22321 ccttcggtca gcggcaaatc tcgaccattt accagccgat gaaccagtac aaagtggtga
    22381 tggaagtgga tccgcgctat acccaggaca tcagcgcgct ggaaaaaatg ttcgttatca
    22441 ataacgaagg aaaagcgatc ccgctgtcgt atttcgctaa atggcaaccg gcgaatgccc
    22501 cactatcggt gaatcatcag ggattatcgg cggcctcgac catttcgttc aacctgccga
    22561 ccggaaaatc gctctcggac gccagtgcgg cgatcgatcg cgcaatgacc cagcttggtg
    22621 tgccttcgac ggtgcgcggc agttttgccg gaacagcgca ggtgttccag gagacgatga
    22681 actcacaggt gatcctgatt atcgccgcca tcgccacggt gtatatcgtg ctgggtatcc
    22741 tttacgagag ttatgtgcac ccgctgacga ttctctccac cctgccctcg gcgggtgttg
    22801 gagcgctgct ggcgctggag ctgttcaatg ccccgttcag cctaatcgcc ctgataggga
    22861 tcatgctatt aatcggcatc gtgaagaaaa acgccattat gatggtcgat tttgcgcttg
    22921 aagcccaacg gcacggtaac ctgacgccgc aggaagctat tttccaggcc tgtctgctgc
    22981 gttttcgccc gattatgatg actaccctgg cggcgctgtt tggcgcgctg ccgctggtgt
    23041 tgtcgggcgg cgacggctcg gagctgcggc aacccctggg gatcaccatt gtcggcggac
    23101 tggtaatgag ccagctcctt acgctgtata ccacgccggt ggtgtatctc tttttcgacc
    23161 gtctgcggct gcgtttttcg cgtaaaccca aacaaacggt aaccgagtaa atgacagatc
    23221 ttcccgacag cacccgctgg caattgtgga ttgtggcttt cggcttcttt atgcagtcgc
    23281 tggacaccac catcgtaaac accgcccttc cctcaatggc gcaaagcctc ggggaaagtc
    23341 cgttgcatat gcacatggtc attgtctctt atgtgctgac cgtggcggtg atgctgcccg
    23401 ccagcggctg gctggcggac aaagtcggcg tgcgcaatat tttctttacc gccatcgtgc
    23461 tgtttactct cggttcactg ttttgcgcgc tttccggcac gctgaacgaa ctgttgctgg
    23521 cacgcgcgtt acagggcgtt ggcggcgcga tgatggtgcc ggtcggcaga ttgacggtga
    23581 tgaaaatcgt accgcgcgag caatatatgg cggcgatgac ctttgtcacg ttacccggtc
    23641 aggtcggtcc gctgctcggt ccggcgctcg gcggtctgct ggtggagtac gcgtcgtggc
    23701 actggatctt tttgatcaac attccggtgg ggattatcgg tgcgatcgcc acattgctgt
    23761 taatgccgaa ctacaccatg cagacgcggc gctttgatct ctccggattt ttattgctgg
    23821 cggttggcat ggcggtatta accctggcgc tggacggcag taaaggtaca ggtttatcgc
    23881 cgctgacgat tgcaggcctg gtcgcagttg gcgtggtggc actggtgctt tatctgctgc
    23941 acgccagaaa taacaaccgc gctcttttca gtctgaaact gttccgcact cgtacctttt
    24001 cgctgggcct ggcggggagc tttgccggac gtattggcag tggcatgttg ccctttatga
    24061 caccggtttt cctgcaaatt ggcctcggtt tctcaccgtt tcatgccgga ctgatgatga
    24121 tcccgatggt gcttggcagc atgggaatga agcgaattgt ggttcaggtg gtgaatcgct
    24181 ttggttatcg tcgggtactg gtagcgacca cgctgggtct gtcgctggtc accctgttgt
    24241 ttatgactac cgctctgttg ggctggtact acgttttgcc gttcgtcctg tttttacaag
    24301 gaatggtcaa ctcaacgcgt ttctcctcca tgaacaccct gacgctgaaa gatctcccgg
    24361 acaatctggc gagcagcggc aacagcctgc tgtcgatgat tatgcaattg tcgatgagta
    24421 tcggcgtcac catcgccggg ctgttgctgg gactttttgg ttcgcagcat gtcagcgtcg
    24481 acagcggcac cacacaagcc gtctttatgt acacctggct tagcatggcg ttgatcatcg
    24541 cccttccggc gttcatcttt gccagagtgc cgaacgatac gcatcaaaat gtagctattt
    24601 cgcggcgaaa aaggagcgcg caatgaagtt ctggcgaccc ggtattaccg gcaaactgtt
    24661 tctggcgatt ttcgccacct gcattgtctt gctgatcagt atgcactggg cggtgcgtat
    24721 cagttttgag cgtggcttta ttgattacat caagcatggt aatgaacagc gattacaact
    24781 gttaagtgat gcgcttggcg agcagtatgc gcagcatggc aactggcgct tcctgcgcaa
    24841 caatgatcgc tttgtctttc agatcctgcg ttcatttgaa cacgataatt cggaagataa
    24901 acccggcccg ggtatgccac cgcacggctg gcgtacccag ttctgggtgg ttgatcaaaa
    24961 caacaaagtg ctggttggtc cgcgagcgcc gattccacct gacggtacac ggcgacccat
    25021 tctggtcaac ggtgcggaag ttggcgcggt gatcgcctcc cccgttgagc ggttaacgcg
    25081 caatactgat atcaatttcg ataaacaaca gcggcaaacc agctggttga ttgtcgctct
    25141 ggcaacgtta ctcgcggcgc tcgccacctt tctgctggcg cgcggtttac tggcaccggt
    25201 aaaacgactt gtcgatggca cgcacaaact ggcggcgggc gatttcacta cccgcgtaac
    25261 gcccaccagt gaagatgaac tgggcaaact ggcgcaagac ttcaaccagc ttgccagcac
    25321 actggagaaa aaccagcaaa tgcggcgcga ttttatggcc gatatttctc acgaactgcg
    25381 tacgccatta gcggtgctgc gcggtgaact ggaagccatt caggatggcg tgcgcaaatt
    25441 cacaccggag acggtggcgt ctttacaggc ggaggtcggt acactgacca aactggttga
    25501 cgatcttcat cagttgtcga tgtctgatga aggcgctctc gcctatcaaa aagcaccggt
    25561 agatttgatc ccactgctgg aagtggcggg cggcgcattt cgcgaacgat ttgccagccg
    25621 tggcctgaaa ctgcagtttt ccctgccaga cagtattacc gtatttggcg atcgcgaccg
    25681 tttaatgcag ttattcaata acttactcga aaacagcctg cgctacactg atagcggcgg
    25741 cagcctgaaa atctctgccg ggcagcacga caaaacggtg cgcctgacct ttgccgacag
    25801 tgcgccaggt gtcagtgacg atcagctaca aaaattgttt gaacgttttt atcgcaccga
    25861 aggttcccgc aaccgtgcca gcggcggttc cgggctgggg ctggcgattt gcctgaacat
    25921 tgttgaagca cataatggtc gcattattgc tgcccattcg ccttttggcg gggtaagcat
    25981 tacagtagag ttaccgctgg aacgggattt acagagagaa gtatgaccga gttaccaatc
    26041 gacgaaaaca caccgcgtat tttgatcgtg gaagatgaac cgaagctggg gcagttgctc
    26101 attgattatc tgcgtgctgc gagctatgcg ccgacgctta tcagccacgg cgatcaggta
    26161 ctgccgtatg tgcgccagac accaccggat ctgatcctgt tagatctgat gctccctggc
    26221 accgatggcc tgacgctgtg ccgggaaatt cgtcgttttt ctgacattcc gatcgtgatg
    26281 gtgacggcaa aaatcgaaga gatcgatcgc ctgctggggc tggagattgg cgcagatgat
    26341 tatatctgta agccgtacag cccacgggaa gtggtagcgc gcgtcaaaac cattttgcgc
    26401 cgttgcaaac cgcagcgcga gttgcagcaa caggatgctg aaagcccgtt gattatcgac
    26461 gaaggtcgtt ttcaggcttc atggcgcggt aaaatgcttg acctgacacc tgcggaattt
    26521 cgtctgttga aaacgctctc tcatgaacca ggaaaagtgt tctcccgcga gcaattgctc
    26581 aatcatcttt atgacgacta ccgcgtagta accgaccgca ccattgacag ccacattaaa
    26641 aacctgcgcc gcaagctgga atctctcgac gccgaacagt catttatccg cgccgtttac
    26701 ggcgttggtt accgctggga agccgacgcc tgccgcatcg tttagtttta gcgacatgac
    26761 tttgttagcc ggagatggcg ttcagaccag atccggcaac attatcccac gcatggtcag
    26821 caaactgaca ttatctctcc gccctggcag gcatcagaaa atcgctcata ctttaatcgg
    26881 taaacagcac ctttagatgc tgttttcgat acgcaatttc aatcaaggag tcattatggc
    26941 tggttggttt gaactcagta agagcagtga taatcagttc cggtttgtgc taaaagcggg
    27001 caatggtgag actatcctca ccagcgagct gtatacctca aaagcctctg cggaaaaggg
    27061 catcgcgtcg gtgcgtagca acagcccgca agaagaacgc tatgagaaaa aaacggcaag
    27121 taacggcaaa ttctatttca atctgaaagc cgctaatcat caaattatcg gctccagcca
    27181 gatgtacgcc accgcgcaat ctcgtgaaac cggaattgcc tccgttaaag ccaatggcac
    27241 aagccagacg gtgaaagaca atacgtaatt acgatgccgg gcgtgggttg ttggcgtccg
    27301 gctttatatc caaataacgc ttagttatta ataattaaga gtaaccgccg ccattaaaat
    27361 agtgggcact gacgagcact cgttgtgctt tctttgttta ccatcccata cttatcctcc
    27421 tgaacatgtt catgtgccaa aatatcttct aatactttcg tgacgctatc cggtttatga
    27481 catacactga tgtaagattt ttacttacag gcgggcgtaa aatggccaga acgatgacgg
    27541 ttgatctcgg cgatgagttg cgtgaattta tcgagtcctt aattgaatcc ggcgactatc
    27601 gcacccaaag cgaagtgatc cgtgagtcac tgcgattact tcgagaaaag caagcagagt
    27661 ctcgcctgca agcgttacgt gacatgctcg cagaagggtt gagtagtggt gaagctcagc
    27721 cgtgggaaaa ggacgcgttc ttacggaagg tgaaagcagg gataaggaaa taatgtgcgt
    27781 attattaaac tcatgccaaa agccaatgag gatttagaag gcatctggta ttacagctat
    27841 caccatttcg gcgaaccaca agccgacaga tatgtggaac atctttcaga cgttctccag
    27901 attctgagta acaataatat aggtacgccc agacctgaat taggcgaagg tatttttgtt
    27961 cttccttttg aacgccacgt catttatttc ctgcaatcac caggggaaat tatcgtcatt
    28021 cgtatcctta accaaaacca ggacgccacc agacatttac actggagcta aacccgccga
    28081 ttgcgctaca atgcccgccc ttaaagtggg ggcactcccc taaccgcttc atcaggtgaa
    28141 gcggatctga cctgtcatca gaacgagaga attatgttta aaccggaact cctttccccg
    28201 gcgggaacgc tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg
    28261 ggccagccgc gttattccct gcgtgtgcgc aacaacgaat tcaaccacga aaatcttcag
    28321 ctcggcatca atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt caacattgca
    28381 ccgcacaacg ccaagctgaa aacctttatc cgtgacctga aaccggtggt ggaaatgggg
    28441 ccggatgcgc tgattatgtc cgatccaggg ctgattatgc tggtgcgtga gcacttccct
    28501 gaaatgccga tccacctttc ggtgcaggct aacgccgtga actgggcgac ggtgaaattc
    28561 tggcagcaaa tgggcctgac ccgcgtgatc ctctctcgcg agctgtcgct ggaagagatt
    28621 gaagagatcc gcaatcaggt gccggatatg gagatcgaga tcttcgttca cggcgcgctg
    28681 tgcatggcct actccggtcg ctgcctgctc tctggctata tcaacaagcg cgacccgaac
    28741 cagggcacct gcaccaacgc ctgccgctgg gagtacaacg tccaggaagg gaaagaagat
    28801 gatgttggca acatcgtaca caagtacgag ccgattccgg tgcaaaatgt tgagccgacg
    28861 ctgggtatcg gcgcaccaac cgacaaagtg tttatgatcg aagaggccca gcgtccgggc
    28921 gagtatatga ccgcgtttga agatgagcac ggcacttaca tcatgaactc gaaagatctg
    28981 cgcgccatcg cccatgtaga acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa
    29041 ggtcgtacca aatctttcta ctattgtgca cgcaccgcac aggtttaccg caaagctatc
    29101 gatgacgccg ctgcgggaaa accgttcgat accagcctgc tggaaactct ggaaggtctg
    29161 gcgcatcgtg gctataccga aggtttcctg cgtcgtcata ctcacgacga ttatcagaac
    29221 tacgaatacg gttattcagt ttctgaccgc cagcagtttg ttggtgagtt taccggtgag
    29281 cgcaaggggg acctcgcggc ggtagcggtg aaaaataaat tctccgttgg cgacagcctt
    29341 gagctgatga cgccgcaagg caacattaat tttacccttg agcacatgga aaacgccaaa
    29401 ggcgaagcta tgccgatagc accaggcgat ggttatactg tgtggctccc ggtcccgcag
    29461 gatcttgagc tcaattacgc gctgctgatg cgtaatttct ccggggaaac cacgcgtaat
    29521 ccccacggta agtgattaat ttcgattatt tttcccggat ggaaaattct tagaaaccga
    29581 tcacatacag ctgtatttat taaggttatc atccgtttcg ctgaaaaaca taacccataa
    29641 aatgctagct gtaccaggaa ccacctcctt agcctgtgta atctccctta cacgggctta
    29701 tttttttatg cataagccct atctctggta accgtcttcc attgaccaca tcgatagaat
    29761 ctcccttcat agcacgatgc ctttcacgta acggcatcgt gctcgcacag gttccggcta
    29821 agcacaacca gaacgcgcat gtttgacgct taccaaaaaa tattctcact ctccacattt
    29881 gaatgtcaga cgagcgacgc catgtaatcc tgcaccttct gtcttcaggt caactatctg
    29941 catttttttg ccctgagtaa cacagaaatg ggctgcatca ttttttacta tattttctgc
    30001 accagatatt ctgcccctgg ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag
    30061 tttacgctga atattacttt tatatgcaag accaaattta ccgatacttg tctcatcatt
    30121 atgcacagca caaccagaca caataaaaat actaattaat gatatagcag ctatcttttt
    30181 catctcacct tcccccatta aataccaacg acactctcta gtgtttaaat ataataatgg
    30241 catgattatt at
//
LOCUS       DAZFRN010000048        29655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFRN010000048 DAZFRN010000000
VERSION     DAZFRN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.048"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(14..361)
                     /gene="yajD"
                     /locus_tag="ACHKUB_002065"
     CDS             complement(14..361)
                     /gene="yajD"
                     /locus_tag="ACHKUB_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO6655086.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(490..1461)
                     /gene="secF"
                     /locus_tag="ACHKUB_002066"
     CDS             complement(490..1461)
                     /gene="secF"
                     /locus_tag="ACHKUB_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO6655087.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(1472..3319)
                     /gene="secD"
                     /locus_tag="ACHKUB_002067"
     CDS             complement(1472..3319)
                     /gene="secD"
                     /locus_tag="ACHKUB_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO6655088.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(3347..3679)
                     /gene="yajC"
                     /locus_tag="ACHKUB_002068"
     CDS             complement(3347..3679)
                     /gene="yajC"
                     /locus_tag="ACHKUB_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO6655089.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(3702..4829)
                     /gene="tgt"
                     /locus_tag="ACHKUB_002069"
     CDS             complement(3702..4829)
                     /gene="tgt"
                     /locus_tag="ACHKUB_002069"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO6655090.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(4885..5955)
                     /gene="queA"
                     /locus_tag="ACHKUB_002070"
     CDS             complement(4885..5955)
                     /gene="queA"
                     /locus_tag="ACHKUB_002070"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO6655091.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            6048..6629
                     /gene="acpH"
                     /locus_tag="ACHKUB_002071"
     CDS             6048..6629
                     /gene="acpH"
                     /locus_tag="ACHKUB_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO6655092.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(6634..8451)
                     /gene="malZ"
                     /locus_tag="ACHKUB_002072"
     CDS             complement(6634..8451)
                     /gene="malZ"
                     /locus_tag="ACHKUB_002072"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO6655093.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEVPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(8607..9980)
                     /gene="proY"
                     /locus_tag="ACHKUB_002073"
     CDS             complement(8607..9980)
                     /gene="proY"
                     /locus_tag="ACHKUB_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO6655094.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(10056..11375)
                     /gene="brnQ"
                     /locus_tag="ACHKUB_002074"
     CDS             complement(10056..11375)
                     /gene="brnQ"
                     /locus_tag="ACHKUB_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO6655095.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(11782..13077)
                     /gene="phoR"
                     /locus_tag="ACHKUB_002075"
     CDS             complement(11782..13077)
                     /gene="phoR"
                     /locus_tag="ACHKUB_002075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO6655096.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(13135..13824)
                     /gene="phoB"
                     /locus_tag="ACHKUB_002076"
     CDS             complement(13135..13824)
                     /gene="phoB"
                     /locus_tag="ACHKUB_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO6655097.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            14014..15216
                     /gene="sbcD"
                     /locus_tag="ACHKUB_002077"
     CDS             14014..15216
                     /gene="sbcD"
                     /locus_tag="ACHKUB_002077"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO6655098.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            15213..18356
                     /gene="sbcC"
                     /locus_tag="ACHKUB_002078"
     CDS             15213..18356
                     /gene="sbcC"
                     /locus_tag="ACHKUB_002078"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO6655099.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            18482..19666
                     /gene="araJ"
                     /locus_tag="ACHKUB_002079"
     CDS             18482..19666
                     /gene="araJ"
                     /locus_tag="ACHKUB_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO6655100.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(19809..20717)
                     /gene="mak"
                     /locus_tag="ACHKUB_002080"
     CDS             complement(19809..20717)
                     /gene="mak"
                     /locus_tag="ACHKUB_002080"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO6655101.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            20842..21753
                     /gene="rdgC"
                     /locus_tag="ACHKUB_002081"
     CDS             20842..21753
                     /gene="rdgC"
                     /locus_tag="ACHKUB_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO6655102.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(21831..21914)
                     /locus_tag="ACHKUB_002082"
     CDS             complement(21831..21914)
                     /locus_tag="ACHKUB_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655103.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(21911..22252)
                     /locus_tag="ACHKUB_002083"
                     /pseudo
     CDS             complement(21911..22252)
                     /locus_tag="ACHKUB_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(22400..22684)
                     /gene="ppnP"
                     /locus_tag="ACHKUB_002084"
     CDS             complement(22400..22684)
                     /gene="ppnP"
                     /locus_tag="ACHKUB_002084"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO6655104.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(22756..23433)
                     /gene="aroM"
                     /locus_tag="ACHKUB_002085"
     CDS             complement(22756..23433)
                     /gene="aroM"
                     /locus_tag="ACHKUB_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO6655105.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(23691..23882)
                     /gene="yaiA"
                     /locus_tag="ACHKUB_002086"
     CDS             complement(23691..23882)
                     /gene="yaiA"
                     /locus_tag="ACHKUB_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO6655106.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(23932..24456)
                     /gene="aroL"
                     /locus_tag="ACHKUB_002087"
     CDS             complement(23932..24456)
                     /gene="aroL"
                     /locus_tag="ACHKUB_002087"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO6655107.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(24639..25097)
                     /gene="yaiI"
                     /locus_tag="ACHKUB_002088"
     CDS             complement(24639..25097)
                     /gene="yaiI"
                     /locus_tag="ACHKUB_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO6655108.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            25217..26026
                     /gene="proC"
                     /locus_tag="ACHKUB_002089"
     CDS             25217..26026
                     /gene="proC"
                     /locus_tag="ACHKUB_002089"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO6655109.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(26043..27158)
                     /gene="adrA"
                     /locus_tag="ACHKUB_002090"
                     /gene_synonym="dgcC"
     CDS             complement(26043..27158)
                     /gene="adrA"
                     /locus_tag="ACHKUB_002090"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO6655110.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(27260..27580)
                     /gene="psiF"
                     /locus_tag="ACHKUB_002091"
     CDS             complement(27260..27580)
                     /gene="psiF"
                     /locus_tag="ACHKUB_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO6655111.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAEKTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(27699..29114)
                     /gene="phoA"
                     /locus_tag="ACHKUB_002092"
     CDS             complement(27699..29114)
                     /gene="phoA"
                     /locus_tag="ACHKUB_002092"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO6655112.1"
                     /translation="MKQSTIALALLTLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDHTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(29215..29475)
                     /gene="iraP"
                     /locus_tag="ACHKUB_002093"
     CDS             complement(29215..29475)
                     /gene="iraP"
                     /locus_tag="ACHKUB_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO6655113.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         7398 a         8001 c         7335 g         6921 t
ORIGIN      
        1 ttttacgttt taatcacttc ttcttgttca tcatcgcttt caggtcagcg aatgggttgt
       61 acttcgcttc accgacatct ttctgcgcat cttcccctgc gataacggtc gtaccatact
      121 gatccgcttc ggtatatttc gaatgctcat gatcgtggca atagagacaa agcaattccc
      181 agttactgcc atcttccggg ttattggtat ggtcgtgatc aatgtggtga acggtaagtt
      241 cacgcaggtt ggaataaaca aactcgcggg aacagcgacc gcagacccac ggatagattt
      301 ttaatgcttt ttcgcgatag ccgctttcta accgcgcata gttttttggg atgatagcca
      361 tgaaaagtta cctggatgaa atgtagggtt acgcgcagtt tcccaagatg cggggatttt
      421 cgcaatctcc atacatcaaa aaaaatcccg atcttctgac cgggattttc aacatcaacg
      481 ggaacttgat tacggcagaa ttgacggctg atccgcccct tctttttcca ctttctgctg
      541 caacatgtgt tcgcgcttca tacccagttt cagagccaac gcagatgcta cgtagatgga
      601 agatgcagtg ccgatggaaa caccgataag catggtcagc gagaagcctt ccagtaccgg
      661 accaccgaag agatacagca tcaggataac catcaaggta gtaccggatg tgatcaaggt
      721 acggtgcagc gtctgggtca aggacacgtt aaagatttcg taaggcgtac cgcgacggat
      781 cttgcggaag ttttcacgaa tacggtccga taccacgata ctgtcgttaa gcgagtaacc
      841 gataaccgac atcaacgatg ccacaatggt caggtcaatc tcgatatgga ataacgacaa
      901 aatacccagc gtaatgataa cgtcgtgcgc cagcgcaata acaacccctg ccgccagtcg
      961 ccactcaaag cgaaaaccta cgtacacaag gatagacagc agcgccgcca tcaacgccat
     1021 cgcaccggtt tgcgcaaggt ctgcccccac gctcggaccg acgaactcaa tacgcttcac
     1081 tgctgcattc tgattggtgg attcgttaat caccttcaga acctggctgc ccagcacctg
     1141 accgccggtt tcgccttcag caggcggcat acggaccatg atgtcgtggc tgctaccaaa
     1201 gttttgcagc attggctctt caaaaccggc tttttgcaat gcatcacgca ttacgtcaat
     1261 ttcagccggt ttttcgagcg taatttcgat aaccgtacca ccggtgaaat ccagccccca
     1321 gttaaagccg cgcacgccca taataacgat agcggcgatt aacagcagac cagagatgcc
     1381 gaaagcccag tagtcccagc gcataaagtc atagacttta cggccgtggt ttagttgttc
     1441 aacagtatat tcctgtgcca catcgcactc ctcagattga cagcttcttg acgcgcttgc
     1501 cgccatatag caggtttacg atggcacgcg taccgacaat cgcggtaaac atcgacgtcg
     1561 ccacaccgat accggtagta atcgcgaacc ctttaattgc cccggtaccc actgcgtaca
     1621 ggatgatgac tttaatcagc gtggtgatgt tcgcatcgaa gatagaactg aatgcgccac
     1681 gataaccttc atcaattgcc tgttgaacag tacgtccgtt gctcaactct tctttaatac
     1741 gttcgttgat cagtacgttc gcatcgaccg ccaccgcaag ggttaagacg atacccgcga
     1801 tacctggcat actcagcgtt gcgcctggca acagcgacat aatgccgacg attaagatca
     1861 agttggcaat cagagcactg gtcgcaatca gaccaaactt cttatagaag atgatcatga
     1921 acagaataga aaccagcaga ccggcaaggc aagcctccag cccctgttca atgttctgca
     1981 tacccagggt tgggccaatg gtacgttctt caacaatctg aatcggcgcg atcaacgcac
     2041 cggcacgcag cagcagtgac agctgacggg cttcgttcgg gttgttgatg ccggtgatac
     2101 ggaagctgtt acccagacga gactggatgt tggcgatgtt aatcacctct tcctgtttca
     2161 ccagaaccgc acgaccattt gcatctttct taccgctgtc tttgtactcc acaaacaggg
     2221 ttgccatcgg tttgccgatg ttgtccttag tgaagttaga catgatgtta ccaccagcgc
     2281 tatcgagcga gatgttaacc tgcggttggt tgtattcgtc ctggctggaa gtggagtcgg
     2341 tgatatggtc accggtcaga attacgcgtt tgtacagcac aactggctga ccttcgcggg
     2401 tctgtttcac ttcagagtcg cccggtacgc gaccggatgc cgcagcggcc tggtcaacgt
     2461 tggtgtttac cagacggaat tccagcgttg ccgtcgcacc cagaatctct ttcgcacgcg
     2521 cagtgtcctg aatacctggc agttcaacaa cgatacggtc agcaccctga cgctgaacca
     2581 ccggctccgc cacgccaagt tggtttacac ggttacgcag gatattgatg ttctgctgca
     2641 ccgcatattc acgcgcttca ctcagacgag catcgctcat taccgcacgc agctggttgc
     2701 tgccctggct actaatcacc aggtccggat ggcgcttgct cagatacgca atggcttcat
     2761 cacgagcttt agcatcgcgg aaagtaatgc tcaggccgta gttgttttct ttacgaacgg
     2821 tggtatacgg gatgcccttt tcacgcagat cgctgcgcag gctatcgata ttttgttcct
     2881 ggagtttgcc aagcgcggtg tccatatcca cttccatcag gaagtgaacg ccgccacgca
     2941 ggtcaaggcc gagcttcatc ggctcagcgt gaatagctgc cagccagcgc ggcgttgccg
     3001 gggcaaggtt aagcgccacg acgtatttgt cacccataac gcccattaat gcttcacgag
     3061 cgcgcagctg ggtgtcagtg gagtcgaagc gcgcaagaat agcgccctct tccagtgcca
     3121 cagacttagc agttattttt tcttcttgta acgttttctg gacctggatc agcgtttgct
     3181 cactggcggc gactccgcgc gcaccagtga tctgaacagc cggatcctca ccaaacaggt
     3241 tgggaagcgc atacagcaga ccgatgacaa tcaccacgat cagcatgacg tacttccaca
     3301 aaggataacg gtttaacacg gcaattccct tagggaaaaa ttttaattac agcgccttca
     3361 tggtgccttt cggcaggacg gcagctacga agtcacgttt aataactact tcagtggtgt
     3421 cattcagcgc gatagcaatg tagccgtttt ccgctacttt ggttacgcga ccaaccaggc
     3481 caccgttcgt cagaacttca tcacctttgg caatggagtc catcagcttt ttgtgttctt
     3541 tggtgcgctt ctgctgtgga cgcaggatca tgaaatagaa aatcagaccg aataccacca
     3601 gcatcaaaat caaagacatc gggctacctt gcgccggtgc acccgttgcc gctaccgcat
     3661 cagaaataaa aaagctcatt aaatttccct cattattaat attaatcaac gttcaaaggt
     3721 ggtacttctc gcccctgacg ctggtaaaaa tcagttacga agctctctaa tttaccctct
     3781 tcaatagcct tgcgtaaacc cgccatcaaa cgctggtagt aacgaaggtt atgaatggtg
     3841 ttgagtcgcg cgcctaatat ttcgttgcaa cggtcaagat gatgcaagta agcgcgtgaa
     3901 taattgcgac aggtgtagca atcacactca ggatcgagtg ggccagtatc gctcttatac
     3961 ttcgcattgc ggattttcac cacgccatcg gtcacgaaca aatgaccatt tcgggcgttg
     4021 cgggttggca ttacgcagtc aaacatatcg ataccacgac gtacgccttc aaccaggtct
     4081 tctggtttac caacgcccat caggtaacgc ggtttgtctg ccggaatttg cgggcataca
     4141 tgctccagaa tgcggtgcat atctgctttc ggctcaccca cagccagacc gccgacagcg
     4201 tagccatcaa aaccgatatc taccagacct ttaacagaaa tatcacgtaa atcttcgtaa
     4261 acgctgccct gaatgatacc aaacagcgca tttttgtttc cgagactgtc aaaacgctca
     4321 cggctacgct tcgcccaacg cagagacatc tccatggagc gttttgcgta atcccagtca
     4381 gcaggatacg gcgtacactc atcaaagatc atgacgatat ccgaaccaag atcgtactga
     4441 atctccattg atttttcagg atcgaggaaa atcggatcgc cgttgatcgg gttacggaag
     4501 tgcacgccct gttcggtgat tttacgaata tcgccaaggc tgaagacctg gaaaccgccg
     4561 gagtcggtga ggatcggccc cttccactgc ataaaatcgt gcagatcgcc gtgcagtttc
     4621 atgatttcct ggcccgggcg cagccacagg tgaaaggtgt tgccgaggat aatttgcgcg
     4681 ccagtggctt caacttcttc cggcgtcatc ccttttacgg tgccgtaggt gccaacaggc
     4741 ataaaacaag gcgtttccac tacgccacga tcaaagacca ggcggccacg gcgtgcgcga
     4801 ccgtcggtgg tgtccagttc aaatttcatt ttttctccac tacgtcagaa aaacagtcca
     4861 acgttttaaa ccagcgccgc ggaattactc cccgacgcgc tcattaattg cctgcggatt
     4921 gtacgtgata aacatcgcat caccgtaact aaaaaagcga tatttctctt ctaccgctgc
     4981 tttataggcg ttcatggtgt gttgataacc ggcaaaggcc gaaaccagca taatcagcgt
     5041 cgactctggc aagtggaagt tcgtcaccag cgcatcgacc actttgtact ggaagcccgg
     5101 atagataaag atttgggtat cgtcgaagaa cggttcaatg agatcgtttt tcgctgcctg
     5161 agccgcgctt tccagcgaac gtactgaagt ggtgccaacc gcaatcaccc ggttaccgcg
     5221 cgctttcgcc gccagtaccg cgtctaccac atcctgcggt acttcagcgt attccgagtg
     5281 catgatgtga tcttcaatgg tgtcgacgcg caccggctgg aaggtgcccg caccaacgtg
     5341 caacgtcaca aacgccatct ccacgccttt ggcgcgcaat ttttccagca aaggctcgtc
     5401 aaaatgcaga ccagcggtcg gggctgcaac cgcgcccggt ttttcgctat aaacggtttg
     5461 ataaagttcg cggtcagcgt cttcgtccgg acggtcgata tacggcggca gcggcatatg
     5521 gccgatactg ttgaggatat ccagtaccga acgatcatca ttgaattcga cttcaaacag
     5581 tgcgccgtgg cgcgcggtca ttgttgcgtt aatactttcg tcatcgccca gcagcagttc
     5641 tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca agaatgcgtt tgtcgtcgag
     5701 catccgctca accagcactt caatcttgcc gccgctggct ttacgcccaa acaggcgcgc
     5761 cgggatcacg cgggtattat taaaaaccag aagatcgccg gggttgagct tatcaagtaa
     5821 atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg tccagcgaca gtaaacgaca
     5881 gctactgcgt tcaggcatgg gatagtgggc aatcagggat tcgggcaatt caaaggagaa
     5941 atcggtaacg cgcatgacac tgactcagac taaaataaga ggcgggtagt ctagtgccgg
     6001 ggcgctctcc ctgcaacctt tacccccttt tggatagaat ataatcgatg aattttttag
     6061 ctcacctgca tttagcccat ctcgcggaaa gctcgctttc cggcaattta ctggctgatt
     6121 tcgtacgcgg aaatcctgaa gaaagttttc cgcccgacgt cgtggctggc attcatatgc
     6181 atcgacgtat cgacgtatta accgacaatc tgccggaagt ccgcgaagca cgggaatggt
     6241 ttcgtagtga aactcgccgc gttgcgccta ttacgctgga tgtcatgtgg gatcactttc
     6301 tttcccgcca ctggtcgcag ctgtcgccgg actttccgct acaggaattt gtctgttatg
     6361 cccgcgagca agtgatgacg attttgccgg actcaccgcc acgttttatc aatctgaaca
     6421 attacttgtg gtcagagcag tggctggtgc gctatcgcga tatggatttc atccagaacg
     6481 tgttaaacgg catggcaagc cgccgcccac gtctggatgc cctgcgtgac tcctggtacg
     6541 atttagacgc tcattatgac gccctcgaaa cccgcttctg gcagttttat ccgcggatga
     6601 tggcgcaggc gtcacacaag gcgttataaa acgttagttc atccataccg tagccgaaat
     6661 ggcaggcaaa gccagaatcc cgtttgtcag ttgcccatgg ccttctttgc gttgccattg
     6721 cgcgacatta agaaacggtg acgcggggag caccacttca caggcctcgc cacggttgat
     6781 tgccaccagt acacgttgct gattcagcac gcggacaaat accactacgt tatcttccgc
     6841 atacagcacc tgacagccgc cacgacgcag cgcctgactt ttcttacgca gcgcaatcat
     6901 tcgctggtac agcgcgaata acgccgtatc ctgcttttcc acctgccagg ggaacggttt
     6961 acggcaaaac ggatcgtttt tgccatccag tcctacttca tcaccgtaat aaatgcacgg
     7021 tacaccaggc caggtgaaca gccagaccac cgccagcggc aggcgcgcaa tatcccgacc
     7081 gagcagcgtt ttaaatcgcg cagtatcgtg gctgtcgagc tgattaaaca tacgtaattg
     7141 ttgttgatga gaaagccctg cgcggtaatt atccatccag gccatacagg tttgggcatc
     7201 aatttgctgc ggatcgtaag agatatcggt attggcaaga aatccccaca acgggaatgt
     7261 gaagccacga tagttcatgg cggcatcttc cacatcggcc tgtaaccatt gccgtgcatc
     7321 gccaaaatgt tcgccgacaa tatacgcttc cggctgggtt tctttcgccg cttcggtgat
     7381 cccagcaacg tgctgcatat tattgcgcgc cccacccgcc tcccccagca tatgcaccac
     7441 atccagccgc cagccgtcca tactccacgg cgctttcagc caatggcgga caatactgtc
     7501 ttccccgcga taaatttcat tcaccagact ttccgactga taatccagct tcggcaagct
     7561 ggcatagcca agccagtcga gcgccgtgcc atcatcacta aacgagtacc agtcgcgcca
     7621 gggcgattca gggttgtgac aagctccccc cgtgccacga ttgtgcctgt caaaccaggc
     7681 atgggaatcg ccactgtggt taaacacgcc atccagcacc agccgcattc ccagctgctg
     7741 cgtattgtgg cgtaagcgca gcaacgcccc atcaccgcca aactgcggat cgacatggcg
     7801 ataatcctcg gtatcgtatt tatgtacgct gggagcttta aacaccggat tgagatacag
     7861 cgccgtcacg ccaagctttt tcagatacgg cagtttttcg cttatcccgt ccagatcgcc
     7921 gccatagaac gttgatccgc ccgcctgcgc cgtgaccggt tcatcccagt cacgcaagat
     7981 gatctcttgt ccggctgcat gatggtaata gacatgatcc tgttcagctt cacgaggaag
     8041 actacgcgca aaacgatcag ggaagatctg ataaaaaatc tgatccgcag cccattgtgg
     8101 gccgatatcc ggtacatcga cggcaaactg ctccagtcgt gccggcggca ttcggctgaa
     8161 gccctgcggt gtaaaccaac gctggcgatc gtgccacagc agtttgaaac tgtaacgccg
     8221 ccggggttgt ccgctggaga gatcaatcgc cgcacgccat gcggtgacgc caggctgctg
     8281 ctgactgcgc tgcttatgca tcgatactga catttcttcg ttatcgtgtt ctgtacgcag
     8341 cataatgcgc tgcggtggga cttcgcccgt cagccacagt gtaatgagca gttgatcttt
     8401 gctttgttta acaaatgggg gcaccggcag gtgccatgca tttaacatca taattcccct
     8461 gtgatgaaaa tctgatcacc ttgccatagc ctaatgcagg atcactcatc atctgaagga
     8521 tatttggggg aggaggacgg gggcggataa ctttcggaat agccggatca ggatttcctc
     8581 ctgaaccggc ttacagaacg caaggattac tggttttcag ccagctgacg atcgtggcgg
     8641 cgtttaaaca tccagccaat caacagcaca acaatccacg cgaaaccgac atacagcgag
     8701 atacgcgtat ccgggtgata accaatcaac ccgataataa agagcaggaa aatcaaaccg
     8761 ccgatggtcg ttgctacccc acccggcact ttaaatttca gcgccttaac ttcttctggc
     8821 ggcaaacggc gacggaaggc aatttgcgac agcaggatca taatccacac ccacaccgtg
     8881 gcgaaggttg ccagcgaagc gatcaccagg aagacgtttt ccggcatgat gtagttcaga
     8941 tacaccgcaa acagcagcgc ggtagtcatc accagcaccg taacccacgg aataccgcga
     9001 cgcgacgttt tgctgaaaat tttcggcgcg ctgccctgct ctgccatacc gtggagcata
     9061 cggcctacgc caaatacgtc actgttaatt gccgacagcg aagcagtcag cacaacaaag
     9121 ttaagaatgc tggcggcaaa ggtaatgccc atatgctgga acgtcagcac gaacgggcta
     9181 ccggcagtgc caacctgatt ccacgggtag atagacataa tgacgaacag cgtaccgacg
     9241 tagaacacca gaatacgcat cggcacggag ttaatcgcac gcggtatcga tttctcagga
     9301 tctttcgctt caccggcggt aatcccgata atttcgatcc caccgtaagc aaacatcacc
     9361 atttgcaacg acatcaccat cccaagccag ccgttactga agaagccgcc gttgctccac
     9421 aggttatgaa taccggtcgg ttgcccgccg ttgccaatcc cccagatgat gatgccgaaa
     9481 ccggcgacaa tcatgatgat gatggtggcg actttaaaga acgagaacca gaattccagc
     9541 tcaccgaaga ccttcacgct catcaggttt acggcgcaaa tgatcagcac cacgctcagt
     9601 acccaaatcc agtgcggcac cgtcgggaac cagacaccca tatagatacc aaaagcggtc
     9661 acatcggcga tggcgacaat aaggatttca aagcaatagg tccagccggt aatgtaacct
     9721 gccagcgggc cgaggttttc ctgcgcataa cgcgagaaag agctggctgc cgggttatgt
     9781 accgacattt cccccagcgc acgcataatg atatacgccg cgataccacc gataatatag
     9841 gccaacaaca cgctcggacc ggccattttg atggcgtctg ccgaaccgta aaacagcccg
     9901 gtgccaattg ctgaacccag tgccataaag cgtatgtggc gggtacttag cccacgcttt
     9961 agcttgttct tactttccat caattcctaa cccatcaaca caataaaaaa ccacggggca
    10021 tcgcagcccc gtggttaaaa caaatgttca gtgatttagt gagcgctgga ggtcacctga
    10081 cgacctgccg cgcgatccca gataatggcc agaaccacca tcaccactgt tggcattaac
    10141 cacgccagac cttgttcggc cagcggtaaa cgctgcgccc aggacggtaa gatatcgctg
    10201 aatgcagatg ctttgatccc gtcgagaata ccaaaaagca ggctgataaa catcggcgga
    10261 gcaatcacgc gggacgaatt atgccaccat gagcgtgtaa aacttaatac aaccagtgcg
    10321 atacacggcg gataaatagc ggtcagcacc ggtacggaga tctggatcag ctggcttaag
    10381 ccgaggttag aaaccaccat cgagaagccg ccgaggataa acaccagcgt acgataagag
    10441 agcggtacgt actgggcaaa gaattctgca caagcacagg tcaggccaac tgccgttacc
    10501 aagcaggcga tgaagattaa cgccgccagc aggaagctac cgccgccgcc aaaggtgtgc
    10561 tgaacgtaag catgcagaat agcagcgccg tttgcagact gatcgaccag cgacgcgctg
    10621 tctgaaccca gacggaacag cgccaggtac agcagagtca gaccaacacc cgccatcagg
    10681 ccagcccaga cggtataacg ggtcagcaga cgcgcttcgg taacgccacg agaacgcgcc
    10741 gcgttaacaa taacgatacc aaacaccatt gcgcccagcg tatccatggt cagatagccg
    10801 ttaacgaagc cgttagaaaa cgcagcgttt tgataagcct cagtcgccgt gctgatagaa
    10861 cccgccggcc agacaatcgc ggcaacagac aggatgacca gcgcgataat tttcagcggc
    10921 gcaaggaagt tgcccacggt atccagcagc ttgcccggat agagcgaaac cagaataacg
    10981 atagcgaaat agaccaggct gtagataaac agcggcagcg cggaatcacc agtcagcggc
    11041 gcaatcccca cttcaaagga aacggtagct gtacgcggcg tagcgaaaag cggccccacc
    11101 gccaggtaac aaaccgttgc cagcagtacg ccagcgactt taccgattgg cgtgctgagg
    11161 ctgtcaacac cgccgccaac ttttgccagc gccactaccg ttagtaccgg cagaccaacg
    11221 gcagtaatga ggaagccgaa tgccgcagtc cagacgtgtt cgcctgcctg caagccgacc
    11281 attggaggga aaataatgtt acctgcgccg acgaacaacg caaatgtcat aaagcccaga
    11341 gcgatgatat cgcgcgatct taattgatgg gtcataaatc atactgcctg tggatgtggt
    11401 gttgtaacgt taaatttttg tgccaatccc tggggatgat acagcggtat atttaaacaa
    11461 ttttagcggg aaatactccc aaccaggcgc ggcgaagaat agtttttcga tttaccgcgt
    11521 taatgcagtc agtatgacag cacctgaagc gcaatttaaa cgcttataac gttttaaagc
    11581 aaggagggat ctaaaaacca gaggtatata gcgataagaa atcaccaacc agtataaaac
    11641 actatttccg tgaaaaagac atggctaatc atgcgaacaa aaaatcacca accgttgtaa
    11701 aatctaacgc ccgtattttg actgcaaaga aaataagcca gcatcggctg gcttatggca
    11761 ataaaagatg acaaaggcgg attaatcgct gtttttggca attaaacgtt ccgggataac
    11821 aaaactgaaa cgtgttcctt ttcctactgt actctcaata ttcaggcgac tttcgtgatg
    11881 attcaccgca tgtttcacga tcgctaaccc taatccgcta ccgccggttt gccgggaacg
    11941 cgctttatca acgcgataaa aacgctcggt caggcgcgga atatgctccg gtgcaatgcc
    12001 cggtccgtta tcttcaacgc taaattcggc accgtgcggc actcgctgcc agcgtacggt
    12061 gatatgcgtg ccttctggag tatgattcac ggcgttatag accaggttcg aaatcgcact
    12121 gcgtagctga tcttcgttgc cagacacttt gaggccgtta tctatctcaa aggtaaatgt
    12181 ctgttttttc tgactcagag tctgagcctc acgctcaaca acgcgcagca tcatcggcac
    12241 atcaaccttt tcattgagca aatgcgtcgg tgcggcttct attttcgaca gcgtcagcaa
    12301 ctgcttcacc agcccttcca tccgctgggt ctgctcgcgc atggtgtgca acgctttttc
    12361 gcgtaccgcg ccttccagcg gctgctcatc catcatctcc aggtaaccct gtaacacggt
    12421 caatggcgta cgtaactcat ggctcacgtt ggcaaaaaag ttacgccgcg ccccttccag
    12481 ttgatgcatt tgcgtgacat cacgcgccac catcagcaac tgtttgtggg tataaggcat
    12541 gacgcgaatt tccagatgcc gcccggtgtt gagcaccaga ttgagcgggc gagaaaaatc
    12601 acgcgttttc agatattgcg taaactccgg gtaacgcagc aggttaagga tgttctgccc
    12661 gttatcttcc ggccagcgca aaccaagaat ttgttgcgcc agaccgttac accagaaaat
    12721 accgccctct tcagtggtca gcaccactgc gtcgggcagc gactccgcgc cgctacgaaa
    12781 gcgtttaatc agattgccca gttcacggcg gcgtttttta tttcgcagct gcatctggtg
    12841 taagccgtat agtagcggtt cccagctacc acgccccggt ggcggggtca tactacgatc
    12901 cacccacagc caccatgaaa ggcgcaataa attccagaaa tgccagataa gcagtcccgt
    12961 taccgatgcc agcaaaaacc agggcaggta accaaaaaat gcacccagga tgaaagccgg
    13021 gaagcagcaa agtagcagct ccagcaccag ccttttccac gacagccgtt ccagcacgta
    13081 agatactcca gttaagaaat cataagccct gctctgcgtc cgatgagcaa ggcgttaaaa
    13141 gcgggttgaa aaacgatatc ctgtaccgcg cacggtctgc accatgcggt catgcccgcc
    13201 tggctccagt gctttacgta ggcgacgaat gtggacatcg accgtacgat cttccacata
    13261 cacgttagtt ccccagacgt ggtttaacag ctgctcgcgg ctgtacacgc gctcaggatg
    13321 cgtcataaaa aagtgcagca gtttaaattc tgtcggcccc atctccagcg gctcttcgcc
    13381 cgccatcact cggtgagatg tcgggtcgag acttaatccc tgcatctcaa tcacctcttc
    13441 caccgccatt ggcgaaatac ggcgcattac cgctttgatt cgcgccacca gctccttcgg
    13501 cgaaaacggc ttggtgatat agtcatccgc gccggtttca aggccgcgca cgcgatcttc
    13561 ttcttcccct ctggcggtca acatcaccac tggaatatcc cgggtcatcg actcgcgctt
    13621 gaggtgtttg atgaactgga taccggagcc gccaggtaac atccagtcaa ggagaattaa
    13681 atccggccag ggttcattca gttgattcac agcactgtca taatcttccg cttcgaccgg
    13741 ctgaaagcca ttttgttcga gcacgaagca gaccatttcg cgaattggag cttcatcttc
    13801 tacgaccaga atacgtctcg ccatgatttg ccctgttgta ataaataggt tgcgatcatt
    13861 aatgcgacgt cattatgcgt cagatttatg acagatttat gaaaagctcg tcgcacatat
    13921 cttcaggtta ttgatttccg tggcgcagaa aaaagcaaat ggcacatctg tttgggtata
    13981 atcgcgccca tgctttttcg ccagggaatc gttatgcgca tccttcacac ctcagactgg
    14041 catctcggcc agaacttcta cagtaaaagc cgcgaagctg aacatcaggc ttttcttgac
    14101 tggctgctgg aaacagcaca aacccatcag gtggatgcga ttattgttgc tggtgatgtt
    14161 ttcgataccg gctcgccgcc cagttacgcc cgcacgttat acaaccgttt tgttgtcaat
    14221 ttacagcaaa ctggctgtca tctggtggta ctggcaggaa accatgactc ggtcgccacg
    14281 ctgaatgaat cgcgcgatat catggcgttc ctcaatacga ccgtggtcgc cagcgccgga
    14341 catgcgccgc aaatcttgcc tcgtcgcgac gggacgccag gcgcagtgct gtgccccatt
    14401 ccgtttttac gtccgcgtga cattattacc agccaggcgg ggcttaacgg tattgaaaaa
    14461 cagcagcatt tactggcagc gattaccgat tattaccaac aacactatgc cgatgcctgc
    14521 aaactgcgcg gcgatcagcc tctgcccatc atcgccacgg gacacttaac gaccgtgggt
    14581 gccagtaaaa gtgacgccgt gcgtgacatt tatattggca cgctggacgc gtttccagca
    14641 caaaactttc caccagccga ctacatcgcg ctcgggcata ttcaccgcgc acagattatt
    14701 ggcggcatgg aacatgttcg ctattgcggc tctcccattc cactgagttt tgatgaatgc
    14761 ggaaagagta aatatgtcca tctggtgaca ttttcaaacg gcaaattaga gagcgtggaa
    14821 aacctgaacg taccggtaac gcaacccatg gcggtgctga aaggcgatct ggcgtcgatt
    14881 accgcacagc tggaacagtg gcgcgatgta tcgcaggagc cacctgtctg gctggatatc
    14941 gaaatcacta ctgatgagta tctgcatgat attcagcgca aaatccaggc attaaccgaa
    15001 tcattgcctg tcgaagtatt gctggtacgt cggagtcgtg aacagcgcga gcgtgtgtta
    15061 gccagccaac agcgtgaaac cctcagcgaa ctcagcgtcg aagaggtgtt caatcgccgt
    15121 ctggcactgg aagaactgga tgaatcgcag cagcaacgtc tgcagcatct tttcaccacg
    15181 acgttgcata ccctcgccgg agaacacgaa gcatgaaaat tctcagcctg cgcctgaaaa
    15241 acctgaactc attaaaaggc gaatggaaga ttgatttcac ccgcgagccg ttcgccagca
    15301 acgggctgtt tgctattacc ggcccaacag gtgcggggaa aaccaccctg ctggacgcca
    15361 tttgtctggc gctgtatcac gaaactccgc gtctctctaa cgtttcacaa tcgcaaaatg
    15421 atctcatgac ccgcgatacc gccgaatgtc tggcggaggt ggagtttgaa gtgaaaggtg
    15481 aagcgtaccg tgcattctgg agccagaatc gggcgcgtaa ccaacccgac ggtaatttgc
    15541 aggtgccacg cgtagagctg gcgcgctgcg ccgacggcaa aattctcgcc gacaaagtga
    15601 aagataagct ggaactgaca gcgacgttaa ccgggctgga ttacgggcgc ttcacccgtt
    15661 cgatgctgct ttcgcagggg caatttgctg ccttcctgaa tgccaaaccc aaagaacgcg
    15721 cggaattgct cgaggagtta accggcactg aaatctacgg gcaaatctcg gcgatggttt
    15781 ttgagcagca caaatcggcc cgcacagagc tggagaagct gcaagcgcag gccagcggcg
    15841 tcgcgttgct cacgccggaa caagtgcaat cgctgacagc gagtttgcag gtacttactg
    15901 acgaagaaaa acagttactt accgcgcagc agcaagaaca acaatcacta aactggttaa
    15961 cgcgtctgga cgaattgcag caagaagcca gccgccgtca gcaggccttg caacaggcgt
    16021 tagccgaaga agaaaaagcg caacctcaac tggcggcgct tagtctggca caaccggcac
    16081 gaaatcttcg tccacactgg gaacgcatcg cagaacacag cgcggcgctg gcgcatattc
    16141 gccagcagat tgaagaagta aatactcgct tacagagcac aatggcgctt cgcgcgagca
    16201 ttcgccacca cgcggcgaag cagtcagcag aattacagca gcagcaacaa agcctgaata
    16261 cctggttaca ggaacacgac cgcttccgtc agtggaacaa cgaactggcg ggttggcgtg
    16321 cgcagttctc ccaacaaacc agcgatcgcg agcatctgcg gcaatggcag caacagttaa
    16381 cccatgctga gcaaaaactt aatgcgcttg cggcgatcac gttgacgtta accgccgatg
    16441 aagttgctac cgccctggcg caacatgctg agcaacgccc actgcgtcag cgcctggtcg
    16501 cgctgcatgg gcagattgtt ccccaacaaa aacgtctggc gcagttaatg gtcactatcc
    16561 agaatgtcac tctggaacag acgcaacgta atgccgcact taacgaaatg cgccagcgtt
    16621 ataaagaaaa gacgcagcaa cttgccgatg tgaaaaccat ttgcgagcag gaagcgcgca
    16681 ttaaaacgct ggaagcccag cgcgcgcagt tacaggcggg tcagccttgc ccactttgtg
    16741 gttccaccag ccacccggcg gtcgaggcgt atcaggcgct ggagcctggt gttaatcagt
    16801 ctcgattact ggcgctggaa aacgaagtta aaaagctcgg tgaagaaggt gcgacgctac
    16861 gtgggcaact ggacgcctta acaaagcagc ttcagcgtga tgaaaacgaa gcgcaaagcc
    16921 tccgacaaga tgagcaagca cttactcaac aatggcaagc cgtcacggcc agcctcaata
    16981 tcaccttgca gccacaggac gatattcaac cgtggctaga tgcacaagat gagcacgaac
    17041 gccagctgcg gttactcagc caacgacatg aattacaagg gcagattgcc gcgcataatc
    17101 agcaaattat ccagtatcaa cagcaaattg aacaacgcca gcagcagctc ttaacggcat
    17161 tggcgggtta tgcactgaca ttgccacagg aagatgaaga agagagctgg ttggcgacac
    17221 gtcagcaaga agcgcagagc tggcagcaac gccagaacga attaaccgcg ctgcaaaacc
    17281 gtattcagca gctgacgccg attctggaaa cgttgccgca aagtgatgag ctcccgcaca
    17341 gcgaagaaac tgtggcgctg gaaaactggc ggcaggttca tgaacaatgt ctcgcattac
    17401 acagccagca gcagacgtta cagcaacagg atgttctggc ggcgcaaagt ctgcaaaaag
    17461 cccaggcgca gtttgacacc gcgctacagg ccagcgtctt tgacgatcag caggcgttcc
    17521 ttgcggcgct aatggatgaa caaacactaa cgcagctgga acagctcaag cagaatctgg
    17581 aaaaccagcg ccgtcaggcg caaacgctgg tcactcagac agcagaaacg ctggcacagc
    17641 atcaacaaca ccgacctgac gggttggctc tcactgtgac ggtggagcag attcagcaag
    17701 agttagcgca aactcaccaa aagttgcgtg aaaacaccac gagtcaaggc gagattcgcc
    17761 agcagctgaa gcaggatgca gataaccgtc agcaacaaca aaccttaatg cagcaaattg
    17821 ctcaaatgac gcagcaggtt gaggactggg gatatctgaa ttcgctaata ggttccaaag
    17881 agggcgataa attccgcaag tttgcccagg ggctgacgct ggataattta gtccatctcg
    17941 ctaatcagca acttacccgg ctgcacgggc gctatctgtt acagcgcaaa gccagcgagg
    18001 cgctggaagt cgaggttgtt gatacctggc aggcagatgc ggtacgcgat acccgtaccc
    18061 tttccggcgg cgaaagtttc ctcgttagtc tggcgctggc gctggcgctt tcggatctgg
    18121 tcagccataa aacacgtatt gactcgctgt tccttgatga aggttttggc acgctggata
    18181 gcgaaacgct ggataccgcc cttgatgcgc tggatgccct gaacgccagt ggcaaaacca
    18241 tcggtgtgat tagccacgta gaagcgatga aagagcgtat tccggtgcag atcaaagtga
    18301 aaaagatcaa cggcctgggc tacagcaaac tggaaagtac gtttgcagtg aaataactat
    18361 tcagcaggat aatgaataca gaggggcgaa ttatctcttg gccttgctgg tcgttatcct
    18421 gcaagctatc actttattgg ctacggtgat tggtagccgt tctggtggtt gtgatggtgg
    18481 tatgaaaaaa gtcattttat ctttggctct gggcacgttt ggtttgggga tggccgaatt
    18541 tggcattatg ggcgtgctca cggagctggc gcataacgta ggaatttcga ttcctgctgc
    18601 cgggcatatg atctcgtatt atgcactggg ggtggtggtc ggtgcgccaa tcatcgcact
    18661 cttttccagc cgctactcac tcaaacatat cttgttgttt ctggtggcgt tgtgcgtcat
    18721 tggcaacgcc atgttcacgc tctcttcgtc ttacctgatg ctcgccattg gtcggctggt
    18781 atccggcttt ccgcatggcg cattttttgg cgtcggagcg atcgtgttat caaaaattat
    18841 caaacccgga aaagtcaccg ccgccgtggc ggggatggtt tccgggatga cagtcgccaa
    18901 tttgctgggc attccgctgg gaacgtattt aagtcaggaa tttagctggc gttacacctt
    18961 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccgga
    19021 tattcgcgac gaggcgaaag gaaagctgcg cgaacaattt cactttttgc gcagcccggc
    19081 cccgtggtta attttcgccg ccaccatgtt tggcaacgca ggtgtgtttg cctggttcag
    19141 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    19201 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    19261 acgttattca ccactgcgca ttgcagcagt gactgacttt ataattgtac tggcactgct
    19321 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    19381 cgcgggatta tttgcccttt cagcaccgct acaaatattg ttactacaaa acgccaaagg
    19441 cggagagtta ttaggtgccg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    19501 cgcatattgc ggtggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    19561 tgccgccctg ctttcgtttg ctgcgatgtc gtcgttgctg ctgtatggtc gctataagcg
    19621 ccagcaagcg gcggatactc cggtgctggc gaaaccactg gggtaggtta tagtctcggt
    19681 gctcattact tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    19741 cattttgcag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    19801 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    19861 tgcttcgcct tacgcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    19921 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    19981 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    20041 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggctgataat ttcactgcct
    20101 ttcagcgcat gtccgctcaa acgacgataa tctgtggcaa atcccgtgcc cgaaataaag
    20161 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    20221 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    20281 atatgcgccc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    20341 aatactgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    20401 gcgtcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    20461 tgaccgttga gccaggttga attggcattc ttaaccacac cggtgtaagg cgaaattgag
    20521 ccaggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    20581 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    20641 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    20701 tcgataccta tacgcacggt actctcctta tttttttcaa tatcaatagc gtagagacgg
    20761 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    20821 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    20881 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    20941 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    21001 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcacgcaa agaagaaaaa
    21061 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa gctggaagcg
    21121 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    21181 tctctgctgc cgcgagcttt cagccgtttt agccagacaa tgatgtggat cgacacggtt
    21241 aatggtttga ttatggtgga ctgcgccagc gccaaaaaag cggaagatac gctggcatta
    21301 ctgcgtaaaa gcctggggtc gttacctgtt gtaccattga gcatggaaaa cccgattgaa
    21361 ctgacgctaa ccgaatgggt tcgctccggt agcgcagcac agggcttcca gttgcttgat
    21421 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    21481 ctgaccagcg aagagatcac caatcacatc gaagccggaa aagtggtgac taaactggcg
    21541 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctg
    21601 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    21661 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    21721 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    21781 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggcatataa ttattcgcta
    21841 cggcgggcaa taatttttag cgcagcaata ttatgcgttt tatgctgtaa cttgctccat
    21901 ggacgttgtg tcattgtttt tcctcaagcc agtgacgcgc ttcactgcga attttccgca
    21961 ttgccccggt tttcactaaa tcccgtaaca gcgaaagatc gggttgttcg acaaataaga
    22021 gtaatgatgg atcctctttt aaccacgtcg ttttcacatc ggcagcgagc tgtggattat
    22081 tcatagctag cgaccgatgc cgagactcgg tcaatgtcgt taacaacgac gccggggtat
    22141 gtgcgttgcg taatgccgtt tggcgcaaat gatcattgcg actttgcagc aatgcacgcc
    22201 accattcatc atctgattta tcttttatcg tagccagttc cgcaacaata tctggagatc
    22261 ttcactgata tttatatcaa ccttgatgaa ttatgaatat atcggaaatt gaaaaaatcg
    22321 cagcaaagga aatattatta tgggttaaag agaggatatt tcagaaaaat gccctcccgt
    22381 tcaaggggaa ggcgaggaat tacagatagc ggcacagata agaggtgggt tcggcaactt
    22441 gcagatgaaa ctcactgtga ccgggaacat taaacaccga accggcttca tacacctgcc
    22501 agtcggtcgc gtcaggcagt aacacattca gcgcgccact gattaccgtc atctcttccg
    22561 gctcagcggt gctgaaggtg tattcgcctt caaccataac acccacgctg gcgcgaccag
    22621 tgctgctgct ggaaaagccg attgatttca ctttgccgga aaagtactca ttactttgaa
    22681 gcataaactg gccctgtctg gtagttgaac atctacttca ctataggggc cagaggcgct
    22741 ggctgtcacg caaaattaca ccagtaattc cgcagccagc cgtgcaatca atacgttaga
    22801 cagcaagaca ggaacatcga gctgtttttg cagtaaatcg cgatgacgtt ggtgaaatcc
    22861 taaacaatcc agcatgatga catcagcccc ttttgccagt aattctttcc cggcatcaat
    22921 gattttttgt tctgaatcat gaatggggtt acccaatgaa aataccggcg atttctgcaa
    22981 aatttgccat ttttgcgcct gaacggtcag catctcctca accggaacga taacccccac
    23041 ctgatgatct tcaacaatag aggaaaccag tggaggcaat attcgcgacg gctcaagaaa
    23101 gatcgtatta cgcgcagtca tactactaat gtttgctgta ctcattaata aaatgacgtc
    23161 ataaccctga ttatcgagca cttcaaccac accttgcagg tctcgctcca ctttacgacg
    23221 cgaaacatgg gccagctggt tgtcatttaa taatgtgaga atggtgtctt cgcctgcttc
    23281 tggcgcgtac tccgccatca cttcttcacg acttaacttc cccagcaggc tatgatggga
    23341 aatattatct tcgtcaatgt attccgtcag gagcggcaaa acctcctgca taggtacaat
    23401 gccgatggtc aggatcgcca acgacgcact cattgttcat ccaccttact tttctttcgt
    23461 cgttaattac cgggcaagtg tgaagcacca ctgctgacac ttacttgtgc tacaaatgac
    23521 aaaaagcgta gcagcagacg tgcacggcat aatgtaaaga ttccaaatga ttccagtaat
    23581 ggattgttat tgtttaatat tctaattatt agaaaaacat gaattatgaa aaatgtgacg
    23641 cagatccctt gtagtggcac tcagaatccc ttccgagcag tctgatgcgg tcactctttg
    23701 tcagggtcct catagcgttt tttcgcatcc atcgcttcct gagccgtcgg atgttcactg
    23761 atcaacgagt ctggtttagg atgatcggct ctgagttggt accaggttac cgtctgtcct
    23821 ggctttcctt tttcaatcgt cactatatat gcttcacgag gataaggcgg tttcgttggc
    23881 atcgttcttc cttatttcac gggatgaacg ttaagtatag gcgctctaaa atcaacaatt
    23941 gatcgtctgt gccagggcgc tacgaatttc agaaatcacc tggctgggtt cgtttgttgc
    24001 gtcgatgata atatgcgcaa cttcgcgata tagcgcatcg cgttcttcca gcacttcctg
    24061 aacttcttcg ctcagcggtt ttcccgttaa ggttggccgt aaatcttctt ccggtgcagc
    24121 ttgcagtcgg ttaaccagga ctgatactgg cgcacacaaa taaaccacga tcccgttatt
    24181 ttgcatgaag tgacgattaa attccgtcag aataatgccg ccgcctgtag cgataacggt
    24241 ggatggcgca gttaccgctt ccagcgccgc cgtttctctg gcgcgaaatc ccgcccactc
    24301 ttccctttcg acgatctccg cgaccgtcat attgagctgt gattgcaacc actgatcggt
    24361 atcgacaaac cgacggttaa gcgaatcggc aagggccatt ccgaccgttg ttttaccaca
    24421 gccccgaggc ccgatcagaa aaagaggttg tgtcatcgtg ggttttcccc aataggtcgc
    24481 aatgcggcga aagccggtgt catgagaata gcgatcatac catcaaacta gtacaatttc
    24541 gattgtaaag aaaaaattcc acttaaagtg aaaatctcaa tacatccctt actataccaa
    24601 taaatattca agaatgaagt gtaaataata aattacattt agccacgact acgttgcact
    24661 tccagccacc acttctccag ctccgcggca aaggcctggc ggtcacgttg tgaaaggcta
    24721 tctggtccgc cggtctggat cccactggca cgtaaggtat ccataaaatc gcgcatcgtc
    24781 aggcgctcac gaatggtcgc tggcgtgtaa cgttcgccgc gcggattaag cgccgctgcg
    24841 cctttctcga tggcttcagc agccaaaggt atatctgcgg tgatcaccaa atcgcccgct
    24901 tcacactgcc ggacaatttc gttatcggca acgtcgaaac ctgccgcgac gcgcagcgta
    24961 cgaataaatc gcgatggcgg cacgcgtaaa ctctggtttg ctaccagtac cagcggcatc
    25021 tgcatacgtt ccgccgcgcg atacaaaatc tctttaatta cattgggaca cgcgtcggca
    25081 tccacccaaa ttgtcataaa gtcatccttt gttgggtaat cctctattgt gtcgcgcttt
    25141 tgccttccgg catagttctg tttatgcttc tgccagcgat tatcaaaaca atgaatttca
    25201 cggcaggagt gaggcaatgg aaaagaaaat cggttttatt ggctgcggca atatgggaaa
    25261 agccattctc ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa tctgggtata
    25321 caccccctcc ccggataaag tcgccgccct gcatgaccag ttcggcatca acgccgcaga
    25381 atcggcgcaa gaagtggcgc aaatcgccga catcattttt gccgccgtta aacctggcat
    25441 catgattaaa gtgcttagcg aaatcacctc cagcctgaat aaagactctc tggtcgtttc
    25501 tattgctgca ggtgtcacgc tcgaccagct tgcccgcgcg ctgggccatg accggaaaat
    25561 tattcgcgcc atgccgaaca ctcccgcgct ggttaatgcc gggatgacct ccgtaacgcc
    25621 aaacgcgctg gtaaccccag aagataccgc tgatgtgctg aatattttcc gctgctttgg
    25681 cgaagcggaa gtaattgctg agccgatgat ccacccggtg gtcggtgtga gcggttcttc
    25741 gccagcctac gtatttatgt ttatcgaagc gatggccgac gccgccgtgc tgggcgggat
    25801 gccacgcgcc caggcgtata aatttgccgc tcaggcggta atgggttccg caaaaatggt
    25861 gctggaaacg ggagaacatc cgggggcact gaaagatatg gtctgctcac cgggaggcac
    25921 caccattgaa gcggtacgcg tactggaaga gaaaggcttc cgtgccgcag tgatcgaagc
    25981 gatgacgaag tgtatggaaa aatcagaaaa actcagcaaa tcctgatgac tttcgccgga
    26041 cgtcaggccg ccacttcggt gcggttacgt ccggctttct ttgctttgta aagcgccaaa
    26101 tctgccgatt tcaaccactc acgatagtga ctcatttgtg ggttcagcgg cgcaaccccc
    26161 acactaatcc gtaaagttac ctgtggcgta ttcggcaagc gtaacgtgtt tagcccttca
    26221 cgcacccgta acatggcggt aatggcgctc tcagctggcg taccggacat gattactgca
    26281 aactcatcgc cgccaaaccg accaatcaca tcgctaccgc gcagggtaat ttgtaactgt
    26341 cgggtaagcg ccacaatcgc ttcatcgccc acatcatgac cccaggtatc gttgatgctc
    26401 ttgaaatggt cgatatcgat aatcagtaac gttgcatcgc gattatgccg ccgacagtta
    26461 tcaaattcat tgcgtaacat agtttcccaa tgacgtcggt tatacacgcc cgtcatgccg
    26521 tcgcgggtac tcatgacctg caacctgcgt ttatgttccg ccagtttggt tgccgtctgg
    26581 tagctgaccc agccaaacag cagaggataa acgacaataa tgggaaggga gagccaccat
    26641 tccagcggcg cactattgaa cgacacggta atgcccgtaa gctcgagggt gacaaggcag
    26701 gaaaccacca tcaacaccag acccgcgaca aacagacggg ggccgcctgc ccccatcaaa
    26761 ttcagacaca taatcatcaa catagcggtg gaaggcagca cgtttacgcc cattacgcct
    26821 acccacattc ccgctaatac tgcatcggtt tttaagttgt aaatttcccg gctaagcgga
    26881 tcgacggccc tgctcgctat ctgccaggct aaatgcggcc agacgaacgc ccagccgacc
    26941 aacaccagcc accaccagcc cggcggcggg tgcgaaacca gcgttgaagc aatcggtaag
    27001 aacatgccag ccaggccaac cgcacggggt agtctgacgc gacgggcgaa gcgcagcccg
    27061 gaccgctgat gttcgttttg aggagttaaa ggaggttcct ccccatgcgc cgccgctttt
    27121 ttgaaaaagt tttcatcatt cattattttt gggaacattc agaaacaatt ttcccaaatt
    27181 atagagacgg ggctaaaggc agaagtatga aatttcgggt gagccacttt cgtagctcac
    27241 ccgttagcac tatcaggtat caggcggctt tcttgaggca ggcactcata aacttattac
    27301 gatcatcacc tttcagagat tgttgcgttg cttgattatt acattcgcgc atcttttgct
    27361 gctgtggcgt caaacttttt tcaccaggcg cagacttgct gttcttcagg caatcactca
    27421 tgtaggtctt acgagcatcc cctttcaacg cctgcgccgt cgcctgctga ttacaggagg
    27481 tcatacgctg ttgttgtggg gttaaggttt tctcggcagc gccgacggtg gttaaaaaaa
    27541 ccagaccaaa aagcaaggta accagtaatg ttattttcat agcaccatcc ctcttcatgt
    27601 tttaaccatg agcgtttgcg cccgtgatct gccattaagt ctggttgcta acagcaaaaa
    27661 aaccacccgg cagcgaaaat tcactgccgg gcgcggtttt atttcagccc cagagcggct
    27721 ttcatggtgt agaagagatc ggtctggtcg gtcagtccaa caacattggc ggcatgcggg
    27781 ccatacgccg caatacgcaa ctgactgccg gtatgttctt gtgaatcctc ttcagagttc
    27841 ccgtaactca tcaccatcac tgcgccatct ttagtattta gcgcctgagt gaggcccggc
    27901 gctttggtat ccggcgcaac aacctggctg gcgtgggcgt gatcagcggt gactatgacc
    27961 agcgtgttac cctccttttt agcgaattcc agcgcccgtt gtacggcttc atcgagatcg
    28021 accgtctcgc caatttgccc acaaggattc gcagcgtgat cctgtttatc gattgacgca
    28081 ccttcaactt gcaggaaaaa tcctttctca tttttactca acaattcaat ggctttgtcg
    28141 gtcatctgtg ccagggttgg tacactgtca ttacgttgcg gattaggcgt acaggtgact
    28201 gcgggcttat cgatattgcc gtggtacgtt gctttcggcc ctagccagcg cactggcata
    28261 ttgccgtcag caaacaggcc aagcaggggt ttttgctgat tcgcttccgt caccgaattc
    28321 agtgaggcag catcgttcac caactgataa ccacgcgcct gtgcctgttc acgcagcgtt
    28381 tttccctgcc attcaccagc ggttgccgtt tcagcaaagg tttttgcgcc gccgccaagc
    28441 gtaacgtcgg cacgagcgtt aagcagctgt tcggtaatcg atccttttcc gcctttttcc
    28501 agagcgttac ccggacattt ttcactggtc gcgctcggac cgtagcattt gcgcgaagtc
    28561 acatgtgcca ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga aacgttaccg
    28621 gtcgccagac ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt ttcgtgaata
    28681 tcgacgccca gcgcgccgtt ataggttttg acaccggttg accaggcggt tgctgatgca
    28741 gccgagtcgg tgacgtagtc cggtttgcca gtttttttat tcagcgcata gtgagtgtat
    28801 tgcccggtaa gcggtaaggc atcgatacct ttaaaaaagc cgcccgcacc ttcggcataa
    28861 ttacgtgcgg cagtaatttc cgaatccccc atcccatcgc caatcagcaa aataatattt
    28921 tttgcaggtt tatcgctaag agaatcacgc agagcggcgg tatgatcacc cgttaagcgg
    28981 cgagcaccgc cgggtgcagt aatatcgccc tgagcagccc ggttttccag aacaggcatt
    29041 tctggagtcc gggcttttgt cacaggggta aacagtaacg ttaagagtgc cagtgcaata
    29101 gtgctttgtt tcactttatt ttctccatgt acaaatacat taaaaaataa aaacaaagcg
    29161 actataagtc tcggccgtga caactttatg acagctgttg aaaagattaa ctttttactg
    29221 acgaggatgc ttcaataact tctttacgta atcgcgcagc agctccgtat cgtcgtcagg
    29281 aatgctggca tcgggcttta cctcgtacag cgccccctct acctgatcaa tcaaccgctg
    29341 ttggtcattt tgcgccatat tgcgaagcat tgcagtgacg ataatctcca aagcttctac
    29401 ctgcgcacac agttctttcg actcttcttc tttttgggca agcttaaata acaactcagc
    29461 aatgagattt ttcatgtctg tatttcctta tccaaagtat ggagaagtta acattatgtt
    29521 tcacaacttc atagtgtctg gaagtattat ttttatgaca aaggaaatac tttgcgcaaa
    29581 tatttttgtt tgattaccag cgaaacgttt ctcttattta aataattacc cacacaaaat
    29641 ataggctttt aatta
//
LOCUS       DAZFRN010000049        29208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFRN010000049 DAZFRN010000000
VERSION     DAZFRN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.049"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..148)
                     /locus_tag="ACHKUB_002094"
     CDS             complement(<1..148)
                     /locus_tag="ACHKUB_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6655114.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(428..811)
                     /locus_tag="ACHKUB_002095"
     CDS             complement(428..811)
                     /locus_tag="ACHKUB_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655115.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
     gene            complement(914..1375)
                     /locus_tag="ACHKUB_002096"
     CDS             complement(914..1375)
                     /locus_tag="ACHKUB_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6655116.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQEEARQQKRGLWADSAPVPPWVWRSRK"
     gene            complement(2117..2320)
                     /locus_tag="ACHKUB_002097"
     CDS             complement(2117..2320)
                     /locus_tag="ACHKUB_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655117.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            complement(2474..3031)
                     /locus_tag="ACHKUB_002098"
     CDS             complement(2474..3031)
                     /locus_tag="ACHKUB_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO6655118.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            complement(3134..3994)
                     /locus_tag="ACHKUB_002099"
     CDS             complement(3134..3994)
                     /locus_tag="ACHKUB_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6655119.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(4053..4799)
                     /gene="traX"
                     /locus_tag="ACHKUB_002100"
     CDS             complement(4053..4799)
                     /gene="traX"
                     /locus_tag="ACHKUB_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO6655120.1"
                     /translation="MTTDNTNTTRNDSLAARTGTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRHANIRQSAINRLWGWAVIAQF
                     AYYVAGFPWYEGNILFAFAVAAQVLTWCETRSLWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALLACLLAVIPALNLASSDVAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWPGDFFPVFYACHLTVLGVLAV"
     gene            complement(4819..10089)
                     /gene="traI"
                     /locus_tag="ACHKUB_002101"
     CDS             complement(4819..10089)
                     /gene="traI"
                     /locus_tag="ACHKUB_002101"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO6655121.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMLTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVSRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKISLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKIPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVLEPGADREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVWIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESVLRESQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            complement(10089..12278)
                     /gene="traD"
                     /locus_tag="ACHKUB_002102"
     CDS             complement(10089..12278)
                     /gene="traD"
                     /locus_tag="ACHKUB_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO6655122.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHSGESFTIRDWMRGVREDKQNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQ
                     PQQPQQPQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMTAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(12335..13066)
                     /locus_tag="ACHKUB_002103"
     CDS             complement(12335..13066)
                     /locus_tag="ACHKUB_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655123.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYSPMIQDNTATTKDYLYSNNI
                     DADLLVVIGGLIVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAQEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDRINKTSRVSPDSGHFYEPGTALDYRERVRLFVQIQYSHESDWSEILRLTDIV
                     LEKVSSGRPAGGYHHRYTGYTWFVL"
     gene            complement(13234..13494)
                     /locus_tag="ACHKUB_002104"
     CDS             complement(13234..13494)
                     /locus_tag="ACHKUB_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655124.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            complement(13554..14285)
                     /gene="traT"
                     /locus_tag="ACHKUB_002105"
     CDS             complement(13554..14285)
                     /gene="traT"
                     /locus_tag="ACHKUB_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO6655125.1"
                     /translation="MKMKKLMIVTLVSFTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVSSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSEVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(14334..14813)
                     /locus_tag="ACHKUB_002106"
     CDS             complement(14334..14813)
                     /locus_tag="ACHKUB_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO6655126.1"
                     /translation="MKNLAHIALITVIQLIACYLADWGCAETVFILCFIVLWQGLFIW
                     LFSQIRKKRHVSDEFKFSKGVWYILMPVSSLFSPLLSLMVFIIGLLYELRRVCGCVSV
                     REWMRSQVNEKTNEDLHLDFDNMDFHRTNPATGLPMLGIGVDIGGNLYGYSSKRYDE"
     gene            complement(14835..17657)
                     /gene="traG"
                     /locus_tag="ACHKUB_002107"
     CDS             complement(14835..17657)
                     /gene="traG"
                     /locus_tag="ACHKUB_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO6655127.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMVADDPYMVIFSRPSPLRGVYDSNNNFVSCKDASVTLK
                     DKLNLDTKTGGKTWHYYVQQLFGGRPDPDLLFRELLSDSYSYFYGASQSASQIMRKNV
                     TINALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSVGHVIMRNLPMVQTILTG
                     ITIGIFPLLVLAAAFNKMTLPVLKGYVFALMWLQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSEVSQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASSSITPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGSMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISDEQATQELASRSTRASAGMY
                     GDAHAEWGIKPKILGVGGGAGVRGGGRAGIDWSDDDAHQASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQDSNIRND
                     VKQQVDNMVTEYRGNIGDTQNSILGEENIVRGQYSELQNHHKTEVLSQDNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKHKQ"
     gene            complement(17654..19027)
                     /gene="traH"
                     /locus_tag="ACHKUB_002108"
     CDS             complement(17654..19027)
                     /gene="traH"
                     /locus_tag="ACHKUB_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO6655128.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQESQQKVCQDIAGESNIFADWAASRQGCTVGGQSSAVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDIVRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTISKDRALKSQIAKLLTSIQNKALSDSPLAENEKTF
                     IQSTTIPVFRYLVDPLMLGVSNSVLYQLTDYIGYDIMLQYIQELLQQARAMISTGNYP
                     QAVLDSVLDNLNQAQQQIAIFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGGVQ"
     gene            complement(19014..19406)
                     /gene="trbF"
                     /locus_tag="ACHKUB_002109"
     CDS             complement(19014..19406)
                     /gene="trbF"
                     /locus_tag="ACHKUB_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO6655129.1"
                     /translation="MKENKKNQEFKIRKIKRDIERSCDNAIKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(19360..19674)
                     /gene="trbJ"
                     /locus_tag="ACHKUB_002110"
     CDS             complement(19360..19674)
                     /gene="trbJ"
                     /locus_tag="ACHKUB_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO6655130.1"
                     /translation="MRNKQVVLLIAGISGVATGIIVSLNIPFIRQGLFYPASPVEIVV
                     SLCLTFSVSVVFFVGAIVGWILVSEIYYSRMTGLNESSEISEETYNERKQKESGIQNQ
                     KN"
     gene            complement(19664..20218)
                     /gene="trbB"
                     /locus_tag="ACHKUB_002111"
     CDS             complement(19664..20218)
                     /gene="trbB"
                     /locus_tag="ACHKUB_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO6655131.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTDKTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYG
                     FSVFPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGA
                     TDAAGFMARMDTVLQMYGGKTGAK"
     gene            complement(20205..20486)
                     /gene="traQ"
                     /locus_tag="ACHKUB_002112"
     CDS             complement(20205..20486)
                     /gene="traQ"
                     /locus_tag="ACHKUB_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001732192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO6655132.1"
                     /translation="MSKYKFSLPRLDITGVWVFSLGLWFHIVARLVYRKPWMAFFLAE
                     LIAAILVLFGAYQILDTWIARVSREEREALEARQAAILAAEKESRHVSH"
     gene            complement(20552..20953)
                     /locus_tag="ACHKUB_002113"
     CDS             complement(20552..20953)
                     /locus_tag="ACHKUB_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655133.1"
                     /translation="MDKENSSIPERWLIASGVAVILLTLLAFMSFPEPDVSMNRYYSW
                     LMNVLILSVTGVMYVALSVVMVILYFLYERNSRLLYLSVLALIIAAGIWLLRFLDNFL
                     MTPAFHVDLFVLWVLLCFLYIRCKNEMSIPE"
     gene            complement(21084..21449)
                     /locus_tag="ACHKUB_002114"
     CDS             complement(21084..21449)
                     /locus_tag="ACHKUB_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655134.1"
                     /translation="MNFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCSLSMM
                     MAEGIVIMKYELWHIPPALLGLAGVLSWLLLLLHTGYFSGISEGVHKRLWLAVASVFS
                     GAGMIIFYWLLQPSETIMK"
     gene            complement(21463..22206)
                     /gene="traF"
                     /locus_tag="ACHKUB_002115"
     CDS             complement(21463..22206)
                     /gene="traF"
                     /locus_tag="ACHKUB_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO6655135.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKMKQPEPENKPAPVI
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEQYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(22199..22456)
                     /locus_tag="ACHKUB_002116"
     CDS             complement(22199..22456)
                     /locus_tag="ACHKUB_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO6655136.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFVIFYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(22470..24320)
                     /gene="traN"
                     /locus_tag="ACHKUB_002117"
     CDS             complement(22470..24320)
                     /gene="traN"
                     /locus_tag="ACHKUB_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO6655137.1"
                     /translation="MKRLLPLLLGLAVSVAQADSNSDYRAGADFARQIQGQGIGSIQS
                     FNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWATSETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCSRT
                     ARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPVAGTVESASLSAHAGLYFL
                     NSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLTGSGCTANGNCLPHGNGDR
                     MVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGACLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAHSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKSPDCRGIT
                     VDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQIAGQLKQVGQ"
     gene            complement(24317..24739)
                     /locus_tag="ACHKUB_002118"
     CDS             complement(24317..24739)
                     /locus_tag="ACHKUB_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO6655138.1"
                     /translation="MALTKKQREKLRMKFGGRCAYCGCELPEKGWHADHVQAVLRKSE
                     QCMKAAEKRIFRLKSTGDVFRPEADCPENLVPACAPCNLLKTTYSLEMFRTQVSLQVE
                     RGRRSSVNFRTAERFGLIQVVDKPVVFWFEQYQRESEV"
     gene            complement(24765..25136)
                     /locus_tag="ACHKUB_002119"
     CDS             complement(24765..25136)
                     /locus_tag="ACHKUB_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655139.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLTWGWSVKGTFNSDFQLCFRLQPEGPHHWY
                     HRYCRRSLRADFRTLVRLVNP"
     gene            complement(25133..25771)
                     /gene="trbC"
                     /locus_tag="ACHKUB_002120"
     CDS             complement(25133..25771)
                     /gene="trbC"
                     /locus_tag="ACHKUB_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO6655140.1"
                     /translation="MSRKIFVLFALTTGLFISAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKATAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVVATGDCRQVAQGLLDGTGDKSQ"
     gene            complement(25798..26319)
                     /locus_tag="ACHKUB_002121"
     CDS             complement(25798..26319)
                     /locus_tag="ACHKUB_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655141.1"
                     /translation="MGFFSFLRKISDKEKGVFISEENWFSLLEKVSAFDKVRQWAESN
                     GGAYYLLQECRRLTDIEDIRKSEDILNRLEFIDRRFREFISIMSEYNCNDKSKDRWEK
                     WLSEYPPEKIYQVRREGDHLSLKNYGDDITLDRDTAVATVQITVSRKCITQEEMDELY
                     ALSERLKEKCAGN"
     gene            complement(26724..27716)
                     /gene="traU"
                     /locus_tag="ACHKUB_002122"
     CDS             complement(26724..27716)
                     /gene="traU"
                     /locus_tag="ACHKUB_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO6655142.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWSCIFP
                     LSLGSTKVSQGKVPDTANPSMPLQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPEFGKTAQGTAKKEDKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNTIGVDVAVCYEYPSPILP
                     KARWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(27713..28345)
                     /gene="traW"
                     /locus_tag="ACHKUB_002123"
     CDS             complement(27713..28345)
                     /gene="traW"
                     /locus_tag="ACHKUB_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO6655143.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKARVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            complement(28342..28728)
                     /gene="trbI"
                     /locus_tag="ACHKUB_002124"
     CDS             complement(28342..28728)
                     /gene="trbI"
                     /locus_tag="ACHKUB_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO6655144.1"
                     /translation="MTTTQKTTDVTARQRSHWWWTVPGCLAMVLLNAAVSYAIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEVQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGTPDITREIQQDIARRMRAEP"
     gene            complement(28725..>29208)
                     /locus_tag="ACHKUB_002125"
     CDS             complement(28725..>29208)
                     /locus_tag="ACHKUB_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655145.1"
                     /translation="GYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIIL
                     KQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPL
                     SRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
                     "
BASE COUNT         6894 a         8411 c         6674 g         7229 t
ORIGIN      
        1 acttccgtgc ttcgtaggcc agagttacct gcaccactgt atttcccctg tgaatattca
       61 gctcacataa ccgttccctg aatatcagtg aaaaacacaa caccgtggcg cacacggcga
      121 gcaacccgat aagggcatat ttcgtcataa actttgacct ctctgcaagg aagaggctac
      181 aatcagtagt gttcaagtac ttcatgcagg cctcacgagt taatggatta acaagtgggg
      241 tcttcgcatt tctggtacct gcgtcttgcc tgaaccagaa aggctcagac acccgccgcg
      301 ataataccac attccctgtt actgaccatt ccctccattt cccgcttttc actacagaaa
      361 cgccgggggc ggggaaaact tgctttttcc cgtccccggg gttggacaac tgagcaacgc
      421 gaaggcgtca gtggattttt atgatccttc caaactctgc ttccattttt ttcatatcct
      481 gttctcttac tgcgactatc agttgttttc ctgattttcg tgccactctg gcaaactgca
      541 tgacggcaga ttcattattc aaatcaggcc atcctgtaca attaaaaata ataatatgct
      601 cactggcgtt cagtgcatcc ctgataaaaa taaccgcata atcatttaat gttctgtcag
      661 aacaactgta aatctggctg aaatcataat acttataatc agtaatattc agtttattta
      721 aaaagctatc aacttcagaa gctgagtccc ccacaataag aacattatga ttagacgatg
      781 tgattaattc tgatactgcc tgatagttca tttgttcacc tttcattaac agagaaataa
      841 gttaataatg taatatgctt catgctttta aatatcgcat ttcaatgaga actcaatagc
      901 cacagaaata tcattatttc cgtgagcgcc atacccaggg cggaacagga gcgctgtctg
      961 cccacagacc acgtttctgc tgtcgtgctt cttcctgcag gacgggaaga acaggatcgg
     1021 tgttgtactg ctcataaacc catgcagcgc ctgcacggac cataaactga ttaacattca
     1081 taccgtctgg tgcataaacc tgcccgagaa tacgaccata acgatctcgc tgaaaatatg
     1141 tgacagtaac cgtttttccc gcaaccagag atttcattat tttttcagac caccggccat
     1201 agtcctgttt cttttcaggt gcatcaatat taacaagacg gacacgcacc ggatgctgag
     1261 ataacatgac gtcaatggta tcgccatcca gaacacgcac aacacgtcca tgaatatcag
     1321 cagaaaatac ctgacatgaa aaaacaaata ataccactgg tatatatttc ttcattttac
     1381 tctccgtcac tgattacttt tcgtgattgc atgaattcca tgccgggatg aataacggct
     1441 cacgcatcac gccaaaaata aaccgttgaa ttgtgatcaa ttcgacaaaa taagatgagg
     1501 tggcttaata cacatatggc tatgtgttac taaaaacgct ggtcaccccg cctgaccaga
     1561 cacataagaa agaaagtctc atgcattatt cagccttctg atacctggag taacggaggc
     1621 tgctttttct gtcacgtatt tcccgtgacg gcgttaccgg tttccggggg taacatgtga
     1681 cggagattat atttttaatc tccgtgtatt catcatacct gctcttactg ataggataac
     1741 tgtgttatat cgcaggctac cggatgtggc gctgaatata acggtcatag ataagtcata
     1801 catgccctgc tcaggggatt agcgataaga cggaccggga gagtcatctc ccggtttttc
     1861 tttatcctgc cgcgaataca gcccggctgt ctgtcagatt caccggacat aaccgtactg
     1921 ttatgcatgc cactgtcacc ttctgcttct tgaaattcat cacatttttt atccctgttg
     1981 gcttacccgg gatgtgaaat atgttaattc tgtgatgtcc gtttgaaaga cagggcaact
     2041 ccttcttaca ccactataat aataacgatc tgccatataa gtctgatccc ccggcgctcc
     2101 gcaacggggg ttatttttat cggcacttac accggcatgc agatgctctc ttcagggcaa
     2161 aatcccgcca tgcttcacag ggtgccagcc gggccgcatt ctgccaggcc tcagcggctt
     2221 ccctgaaaaa accacgaatc tcctgacgga cggcatgacg ccccacgcgc tgatagcgat
     2281 aaataccgct gtgctcagat aatccactgg atacagccat tttttatccc ccctcttgac
     2341 ccggctcccg gattatgtca gaaacattat gaaacatccc tgaacggttg ggtgtttttc
     2401 tccatcaaaa ctctggatgt ctgttttccc gattttttcg ctgttgccct ctgtcataca
     2461 gagggcgttt ttattattct gccaggatcg cccgcagctc atccttgatc cggttctgtc
     2521 tgcgcacctt ttccaggcgc gcctgcgcat actgttcttc ttcctgtgtg atatgttctg
     2581 tcacatagcc ttccgtatca tagcggcagg cgccggcttt cattgcacac agataacttt
     2641 ccgaacgggt gatggctttc agcgcccggc tgagtttttt atgagagagc gggatattcc
     2701 gctgaaacac ctcatccagt aaaacctccc ggataccaca ggcaagaagc cggggcgtgt
     2761 taccgtcaaa caatcccggc caccagggtt tcagagtgtt caccgcctca tccagcgagg
     2821 gcagcgtcag ataaatggac agcgcctgtc tggcctgtgc tttcttcgcc gccagctctg
     2881 cttcccgggc agccttctcc gccagtttct gctttttcac cttccatttt ggtggcgtgg
     2941 tgacattgat gatggttttc cggctccggg tcggtgtttc cccttccgtt ttccgtttca
     3001 gtgtcagtac cggtcgtttc tgctctgtca tgattgccct gacctttgtg aaaatatgtc
     3061 ccccaggtcg ttgaaactac tcactatgat attaagctga cctcaacgac ctctgacata
     3121 ttaataccga caattaaaga tgttttttaa accattgcgt ctgtactgcc gccacacgtt
     3181 caaaaaacgc gccttcataa atatcataat ggcatgcgtc agcttcctcg tataattcct
     3241 tagtaccaga agcaacagca tcatataagg ctcttccctg ttctggtgga ttaacactat
     3301 cctggccggc aataacaacc aaaactgggc actgtacttt ggctgcgctc tctgccggtt
     3361 tatattgcaa cgtctccata accgtaagga aaggtatttt tatatccatt tcaggatact
     3421 gaccttttac cttctcgaag aatacctttg attcattatc actcagtacc cgggtaaccc
     3481 ccacaaacat ctctttcccg gtatttttct tcttttctgc cattttattc agcgtggaca
     3541 gaaaagaagc tttctccagt tcattcattt ctccggtaac cagaacctca ccgtcagcaa
     3601 aagccagctg gctgacaata cttttcactc ttttatcctg cgcagctgca ttgaatacat
     3661 gacacccacc cagcgatgtt ccccataatc ctatacgctg attatcaata catacctgct
     3721 tttctgccca attgataact gaaataatat cttcggtctg cattgccgga accagacgac
     3781 ctcgttcccc ttcactctca ccgaatccac gataatcaaa agtaatcgtt gcaaacccgg
     3841 cttctgtaaa cgcattcgca aaggaaggca gcagaacatt ccggataccg caaaatccat
     3901 ggcataagag aatcaaagga tgttttatat ttccttccgg gacacgaaat gtcagagcaa
     3961 taccttcagg gagtttgtga tccgtaattt tcataaatat cctgtgttat gttgtttatt
     4021 aataagcaga atcagcgcca gcatcatacc agtcacacag ccagaacgcc cagcacagta
     4081 agatgacagg cgtagaatac cgggaaaaaa tctccgggcc agaaacgggg taatgatttc
     4141 cctgtacacg acaccagacc aacggtcagc accgtcatta caagaccagc tacggccgcc
     4201 acatcactgg aggcaaggtt aagtgccgga ataacggcca gcaggcatgc aagcagcgcc
     4261 agacgttccg ctctgtcttc cgcccggtac agccggtgac tgactgccag catcagcagc
     4321 ccggcaatgc cgtaactggt gccggacaaa ggcccccaca gtgccatcag gagtatggcc
     4381 gcggcagtac gccacaggct gcgcgtctca caccacgtca gcacctgtgc cgccactgca
     4441 aaggcaaaca ggatattccc ctcataccag ggaaagccgg caacgtaata cgcgaactgg
     4501 gcaatcacag cccatcccca cagccggtta atagccgact gccggatatt cgcatgacgg
     4561 gacagattca gtccccagac cagcgcaaac agcggaaacg cgccccgccc ggccagaaac
     4621 atccacgtct gctccagatg cagaatgcgg tttgcatggt ccagcaccat cagcaccagt
     4681 gccaccgttt tgatgatatc ccgctgtccg ggtgaccaga ccagcaacga ctgcagccag
     4741 gtaccggtcc gggcagccag tgaatcgtta cgtgtcgtgt tcgtgttatc cgttgtcatc
     4801 agcgtttgtc ttcctgtatc agtctccgcc cagggttttc tctttctgca ggtcacggac
     4861 catatcccgc tccatctgct gaagtcgctc ctgcagcaga ttttcccggg caacctcccg
     4921 gacagcgtcc tgcacccgtt gagactcacg cagcacactt tccggcagcg cagtttcccg
     4981 ttctgatgac gaagccctgt cctgctcctg cccggcaata gccctgacag cctgctctgt
     5041 tttaccatcc ggctggtcag gtttttcctc tgccattttt ctgacaatct catccgcacg
     5101 gcgttccgtt tcacgacgga tggcctcttc agcctgccgc tgtgccagca cctctgccgt
     5161 gaccggctca ccgtttccgg cccccggctg gacgctgttg tccgggatat ccccccacac
     5221 gcggccaccg gtgatggcac cgggattcca cggacgacct tcaccggcga ttctcaccac
     5281 cacaccgctg tcggggttat cacgggcaat ccagacacca tcctgcatat tatcagccag
     5341 caggctctcg ccgttacggc tgccctgcag ggccacaaac tgcgcatccc cgctgccttt
     5401 cacccggcct tcaccactga atccccgcag cccgtttccg tcatccgtgg tcagtgggtt
     5461 cagccagata ccggcggact tgccgttacg gtcaaaagcc ggcagggcca catacggctg
     5521 cggatatttg cgccccggtg caataaaccg tgccggactg tcgcccccgg ccagtcccgc
     5581 ctggcggaga acagcacggc cagctgccac atcccgcagc tcccgtgccg tactgaacaa
     5641 ccgctctgca ttcatgacct cccggtcagc ccccggctcc agcacatcat gggccgttcc
     5701 tttctgtacg gctttgctga tggcatccgt ccagccctga cggttatcgg tgtacacctg
     5761 cacatgctgc ttcatacgcg acagggccac ataggctgac tcaaagccgg ccatctgttt
     5821 ccggttacct tccgtgcctt caagcgcgat ggcaaaggtt tcactcgccc cctgcgcacc
     5881 gtgagcggtg atggcatagg ccaggtcaat atgttgctct gcctgctcct ggccggggcg
     5941 aatcacccgg gtctgctgtc cgtcagaaag cgtgacactg tcaccggaaa ccgctgtcac
     6001 cgtccagacg ctgttggcca catagccgcg ctcccggtca ctcttcgtga agcgcatccg
     6061 gtcaccggtc cctacccgga tggtgtccgg ggtgtacagt gtgacacctt cagccaccgc
     6121 ctcccggggc gaaatcagcc gcgtgttacc ttccgcatcc tccagggtta tcagcccgtc
     6181 atccttactg ataccggcaa tccggtgata cacattatcc accagggcaa gtgcgtcccg
     6241 atgtgtctcc caggtggaga gacgacgcag ctctccgtca cgtatattcg ctgtgttcag
     6301 gacaggcacc atgacctgct ctttccccag ctcaccggcc ttttcccgta catcatgaat
     6361 catgctgttc agtacgcgcc ggtcctcatt caggtgcgtg acaatcagcg tctgctcccg
     6421 tgcttccggt gttctgccgg tatagtcgcg gacaatggct tcatacagtg tcatggggac
     6481 atccggaaaa gcctcgcctt tcagcatcgc cttctgctgc gcttctgcca gtttcgcttc
     6541 ctggctgtga ctgaactccg tcacggagtg ctccggtgcc catgcgccct cctgacgtgg
     6601 cacctgagac ggtttcacac tctcaagccc ggacagtgcc ctttccacat cccggttaat
     6661 caggctgtat accgcctccc gcagttccgg cgtctgacgc acaatctcct tcatgatgac
     6721 cacatcggca gcactgcgcg tctgctggag acggaaaggc tgaccgggcg cgatagcctg
     6781 cagctggtcc gtgtcaccgc tggccacggc acgaccacca ccggccgcaa tcagggcgta
     6841 tgcccgtgcc atgtcggtat tgcccaccat agagctctca tcgagcagga acagcgtgtt
     6901 gctgaaatcc ggcgtttctc cgctgcgctg ctgcagctgc gtgtcatgca gaaaggatgc
     6961 cagtgtctgt gcatccacgc cggcgctgcg catctcaccg accgcacggt gcgtggggcc
     7021 cagccccacg acacggggac gctcactctc cggcagcatc ttcacggctg acatcaccgc
     7081 ccggaactgt gtggtcttac ccacaccggc ataaccctgc accaccgtga aacggtcgga
     7141 cgtttccagt atcatgcggg tggcggcacg ctgccccgac gtcagtgtct ccatgagttc
     7201 gccgggtacc ctctccatca gcggcgtgac cgcctcctta ccttcgagaa tatggcgaag
     7261 aatgctcttt tctgcctcat acgacgcacg ggaaaccagc aggcctgtgc catagccttt
     7321 tgccacatcc acatacagca aatcaccgcg ttttatctgc gcattgattt cccctcccag
     7381 ttcagtaaaa ctggttcctt ctgcagcaaa cgacttcgcc tctgtcagca ggtccaccat
     7441 gctgaaggcc aggtttttac tttccagcac aggaagggcc agatgaatgg cctgctgtgc
     7501 cggcgtgtga agcccggctt tctgcagact gatagcggtt tccagcaatg tctcaccggc
     7561 acgcgccttt atctgctcag aaaccaccgt aaaggaggga tggcgggcaa gtttttccgc
     7621 agtacgggtt tcatccagtg aggaatacag ccggacatca cgaccgctgc gggccagacc
     7681 gttcagggtg gcgttgtcca tcgccatctg tgtgacggag gcaaaaacct tcgcactgtc
     7741 gctgacggaa tgcccgggcg tttccaccca gccgttctcc agcttcagtg ccgtgaacgg
     7801 tgaatcgctc acaggcaggg tggccggttc agcacgtccc ggcacaacaa ccgtcatcgc
     7861 atcttcactg acggatgcca cctgcaggcg gtcaccgccg gagacgcgga gtccgggaat
     7921 tttccctgtc accctcagac gctcaccgtc tgccaccggt attttttccg gccggaacag
     7981 cgaccagctg ctgtccaggg aggaaatacg caccacctgc gtttcgccct gtgcatcccg
     8041 cagggtcagg ctgtgactct gcgctgtcac ccggtcgata acatagcggt catgactgcg
     8101 tgtctccggg ttccactgct ccatcaccat ccccggacgg tacatatccc gcagataacg
     8161 gctccggctg tccagccaga ccggtgacag tgcagtcatg gtcacctcag ggtgtccgag
     8221 cacgccctgc gttttcagct cactgcgaat ggcctgtgtc agtatggcct gttcccgtac
     8281 cccgctgacc tgtgccacgc tctcttctcc ggctttcacg ctggccgcaa aatctccggc
     8341 cagccgggca tagcggacat tacggtccgg ttcactgatg atggtggccg gtcgctgttc
     8401 tcccccctgc cagcgatatg tgttcacccc ggcatccttc atggccatca gtgcactgcc
     8461 tgtaccggtt cgctgcccgc tgtcggttat caggacctgt acgttatgac gtgcggcacc
     8521 gtccagcagg gttaacgtct ctttcaggga gattttttcg ccctggtcaa cgataacggt
     8581 actgcccggt gtgaaggcca tgccttccag caactgacga cgtccggtta tcagctcacc
     8641 ggacaaccgt tcatcctgct tcaggttcat ctgcgagcga cggtcagcag cgataatctg
     8701 cacctcccgc ccctgctccc gggccatcat gaccagttca gccacccgct cacgctgccc
     8761 ggctgcaccg ccctgcccgg acacaatggc cagcgacgga cggtcctgtg ccagaacact
     8821 gaccgcatcg ctgtaaccag ccgtccggga gacacttttc tccggatgta cggtcacccg
     8881 gttctgtttc atgatgtcac gactgagtgc ccggacggac agttcatcga gcacatgaat
     8941 tcctgatgtg aacagcccct tctcacggtc gagggggata agctgctcac gactgatggc
     9001 ctcatcgata ccggcgcgtg cccgttcaat cacaccattt tccggcggca gtataccgac
     9061 cgttctggcc agcacgtccg tgtacgtaaa ctgcactttt cgttcactta atccggcaat
     9121 cgcctgtgtc accgcctgct gcacatccgg cccgtcctgt gaagcgggtc cgggtgtctg
     9181 cgtgcgggtc tccgcacgct gatccgccgc gtcacgatat gcccggatgt cgaacccggt
     9241 ttccttcagc gtcagcatcc attcagccat tctgacttca ggatcgacgt gctgtttgga
     9301 ttttcgcgta tccagtgccg ccacatcccg ggatttcagc gaggcatcct ccccgacggc
     9361 ctcccggata gcctgtgagc gtccggaaaa ggcctccacc ggtacgcccg gcatctccca
     9421 cataccgtgc ttacccacca cttcggtttc atagcccagc gcctcaacct gctctttcag
     9481 tttttcccgg tagagcctgc caaaggcaat ctgattagcg tacacattct caatgaaccc
     9541 cgttttcccc actttgtcac tgctcagtgt cttccactca ccgttatgct gcgtgacatt
     9601 agccaccacc gcatgcgtgt gtaactgtgg ttcctgatcg cgactggtgt cgtggttaaa
     9661 cagtgccatc accagattac cggtcagtac cgtttctgac tgtccgtccg tcatcacccg
     9721 tgtggaggcc agcgcctcca cctgacggac agcaaagtcc acggcctggt tgtgtgcatc
     9781 aatcaggcgc ttatcgccac ctaacatggc catcatggag acacttttgg gggcggagaa
     9841 ggtcaggtcg tagccgggac gatgcctgtt actgccatcc tgcatgcggc ttagatccgc
     9901 tccgtccggc agccttccct ccagaagacg ggtaaaaaca tccttatcga cactgccctg
     9961 cagtcccagc tgttcagccc ccctgccggc ccagcgttct cccatgctgc ccagtacata
    10021 gtaattatcc ttgtcggtat aatagttacc ggcacttccg gccgatctga cctgcgcgat
    10081 actcatcatc agaaatcatc tcccggctca acatcctccc cacgctcccg gtgcacatta
    10141 atgttcacct cttcacgacg ctgcatctgc tgctggatgt ccggatgatt ttcctgttgc
    10201 catgcctcat aagcggtcat atccaccact tcaccggatt cactgatccc gggtggcagt
    10261 tgctgttcca tctcttcttc cggtttcatt ttcagctcct gctcgatccc ccctgccgga
    10321 acattcacac ctgaatctga cttcttatcg ttgatggcag gagacaccgg ctgttgtggc
    10381 tgttgtggct gttgtggctg ttgtggctgt tgtggctgtt gtggctgttc agcctgagtc
    10441 acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg cccttctgct
    10501 tcccgtgcag caagtacggc actcagacgg ttctccattt ccggattgat gtcacgcgga
    10561 ataaactccg gagcaacctt cggtcgtgcc tgatatttca gggagagttt tactgccgga
    10621 tacggtccgg gcagggtgac ataacaggtc agatccggca gagactgaat gtcggaataa
    10681 ctgaccagcg tctggcgctc catctcctta ccggttgata ccccgtcacg taccgggtct
    10741 gcaccgtagg aatactgctc gctggctttc aggtgctctt tttcaccaat ttcaccggca
    10801 gcgaactctg caatcttatg actcggagag cggaaaaagg ctcgggtatt cagcacatca
    10861 aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata ggactgaata
    10921 ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac cagatccggc
    10981 agtttatgca gcgtgggtaa ctcatcacag aaaaaccaca cacgacggtt acggttttct
    11041 cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac cggcttcagg
    11101 gaggcatggg tgtcggcatt cgacgagata aacaaccagc cgttttgctt atcttcccgg
    11161 acaccacgca tccagtcacg aatagtgaag gactcaccgc tgtgctcaat cccctgcagg
    11221 taacggatgg ctttcacgta gtttgtcagc acagcacgga tggaaatcgc cgttttctcg
    11281 attttctctt ccactaggtt agccgccggt gagttacgca gataggtacg cagtttttca
    11341 attttgatgg aaagcagtgt atccaccagt ttgctgtagc tgcggttggg gtcattacgc
    11401 atcaggtacg ccgcttccgc aaagatggtg cgcccggagc cctgccagaa cgggtcctct
    11461 tttgtgccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg tgtcagacac
    11521 tccttccata aatcccaggc ggcacagcgt gcatccagcg gattcaggat cttatcaatg
    11581 gagggatcat agtaactttt gacaaactca cacgaacggt cataaatcac caccatatca
    11641 ccacgtttac gggcatagtt ggcaagacgt cggataacct ctgacttacc cgtcgatact
    11701 gttccgtgca gacaaaagtt ctggatttca gaatcccgga taatcggcag gtcgccaatc
    11761 cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt cggattgtca
    11821 gtcagctgac gaccacctgt gacttcattt tcgctctgct gtttaccctg acgccccaga
    11881 atccaggaga caacaaagaa ggtgatcagg cagataacca gggccacgac agacgccaga
    11941 acgaatgcag accatagctg ctcaccgcac cagatcatgt atttatcatg cagtacctga
    12001 gccgcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg ctgggacctg
    12061 attaaatccc gcatgccttc cagcgtggta caccaccagt aaatacagcc gttcacaaac
    12121 gtctgccagc ttatttttac ccataaaacc agaccaacga gtatccagaa aaaaataaac
    12181 aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat ggacgcaatc
    12241 tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg gtaaagtcag
    12301 gaacagagca gaaaaagaga ggtgtggaat acattcacag aacaaaccat gtataccctg
    12361 tataccggtg gtgatagccg cctgctggtc tgccggaaga aactttttcc agcacgatat
    12421 cagtcaggcg caatatttca gaccagtcac tctcgtgtga atactggatc tgaacaaaaa
    12481 ggcgtacccg ttcacgataa tccagagccg tgcccggttc ataaaagtgg ccactgtcag
    12541 gagaaacacg ggacgtttta ttgatgcgat cgtatttatc ggcatgagtc tgtgacgaca
    12601 gagaccaggt aatattttct tcctgctcac catcaatacg gctactcccg ggcgtccagg
    12661 caatttcctg cgcactggtc acgatgatct gtctggagac ttctgccagc tttttggggg
    12721 tacagaatac cggagccagc acacacaggg acaacaaatt acagcggccc tcttccggct
    12781 taagcggatt gctcccggat ttctcaggaa aagatatttt tgtcagtatt tcagttaatt
    12841 ccttatgcct gagagaatta ataaagtaca tgacaagcat catgagaaac aatataacaa
    12901 taagccctcc gataaccact aaaaggtcag catcaatatt attgctgtac agataatctt
    12961 ttgtggtcgc tgtattatcc tgtatcatcg gggagtattc agttacaggc ttctcagtaa
    13021 attgattatt ttcaccgaca tattcagcga tattgtctga tgtcataatg actttctccg
    13081 gaagcttccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc acaaccgttc
    13141 agtgttctca tatttatttc ggaaacgccg ggaagtgaca aaatttattt tgttacttcc
    13201 cggggttgga caacgagcaa cgcgaggcgt cagctatttt cttctgcgac gtccggtcgg
    13261 atattcactc acaggaagat attctgttgc gcttacgccg atctcatatg cagagaacgg
    13321 ttccagtgct gtcaggtatc tctggcaggc tatggccagt tcagaatctc cggttgcatc
    13381 agcaaaccgg taaagttctt ttaataactc ccgacataca tgctcaggat acttttcact
    13441 gtcatagccc agcagaaaga gtacctgcga gagacgagat tctgcacggt tcatgtattc
    13501 atccggggca ggcatatcgg cccggtacaa tctgtccggg ccggatgggt aatttacagg
    13561 atgttcgcga tggatttagc cagctggtct tccagtacag gcttcgcttc ttcaaatttc
    13621 aggttaacct tgttggcatt agataccaca cgggtctggt atttatgttg atttccggtt
    13681 gatgtgctgg tctgcacttt tgcaccggag gttccctgac ggagagctgc aacattatca
    13741 gttctgactt cagatgttgt ccgctctgca atctgcacat ccgtgatcat ggtgtagttg
    13801 atatcctcca ccattgcatc ggcagccata ccaatcaacc ctgtggcaat ccctacaccc
    13861 agcgccgcac cggcagagct ggagttatat cctgtgatgc cggtgcccaa tgctgcaccg
    13921 gcaatggcac cttcataacc gctgttcagc caggtctggg actcccgcaa atccattttg
    13981 tctgccttca ggacattggc ctgaatccag tagtatgcct tgtcaggtga gctaacaacc
    14041 tggtagcctt tggctttaac ggcatcactg atttttgcct gcaatccgct catatccttg
    14101 tcagatgtgt ttttgatttg caggaaaacg gtgcgttcac tggaaggctc cagccagatg
    14161 gtttcactca tctgagtttt gacttcaaga ttacgcttct tgattgctgt gctcatcgca
    14221 ccacaccctg aaaggaccag agtgaaactg accaaggtaa ctatcatcaa ttttttcatt
    14281 ttcatcaatg ttccttcata ttgaataaaa caccataata tacaaattaa gattcactca
    14341 tcatatcgtt ttgaactata accatataaa ttaccaccga tatccacacc aatacctaac
    14401 ataggtaacc ctgttgcagg attagtacga tgaaaatcca tattatcaaa atccagatgc
    14461 aaatcttcat tagttttttc atttacctga gatcgcatcc attctcttac acttacacag
    14521 ccacaaactc gccttaattc gtatagtaaa ccaatgataa aaaccatcag gcttaataac
    14581 ggagaaaata aggaagatac tggcatcagt atataccaaa cgcctttgct gaatttaaac
    14641 tcatccgaca catgccgttt ttttcgtatt tgagaaaaca accatataaa caatccctgc
    14701 catagtacaa taaagcacag gataaaaact gtttcagcgc acccccaatc agccagataa
    14761 caggctatga gctgtatcac cgttatcaga gcaatatgag ctaaattttt catcatcatc
    14821 tcccccatac aatattattg cttgtgctta tcctgatact cctttgctct tttcatcagt
    14881 tcctgtgggc tgtcagcacc aggcattctt tcctgagccg acttttcttc attatattta
    14941 ttatcctgag acaatacctc tgttttatgg tgattttgca gttcagaata ctgccctctt
    15001 acaatatttt cctcgccgag gatgctgttc tgagtatccc cgatattgcc ccgatattct
    15061 gtgaccatat tatcaacctg ctgcttcaca tcgttacgga tattactgtc ctgcgttctt
    15121 tgctcaataa taccctgatg cccctgatgg tctgcaataa tatcctggct gccaccgccc
    15181 gaaggtacgc tctccattcc tttaccgata tcgccacggg attcacgcca ggcgttatca
    15241 acaccaggct gtacctgttc ctgaacaaaa gaccaggcca ttgcccgacg acgttctgca
    15301 atctccggtg aactggtgtt tgtcagaata gcttctgcat cctgcggcgc gtgtttcatc
    15361 acatactgtg caaactgttg tgacaggtct tcactcatct gcccactcat gctttcagta
    15421 cgggaggcca tttcagcata ttcatggctg cgggtcatat tcgtcgtgta ctggtcgtaa
    15481 ctctgtttcg ctgagttcag ggctgcagac agttggtcca ccctggaatc ggcattattg
    15541 tcagtatggc tgccggattc actgaccttg cgactggtaa agtaatcgct tgcctcttta
    15601 aagtcttgtg tagccctggc atcgatatca tgacgggcat catgactggc ccgtgaacca
    15661 ctgctggcct gatgcgcatc atcatcgctc cagtcaatac ctgcccgacc accacctctg
    15721 acacctgcgc caccaccaac accaagaatt ttaggtttaa tcccccattc agcatgagca
    15781 tctccataca tcccggcaga agctctcgta cttctggatg ccagctcctg agtcgcctgc
    15841 tcatcactga tattatgcgc cttcgcatag ctttccactg cgctgcgcat acggctggcc
    15901 atcatggagt cctgcgcgct catcgtgctg tcagcgccac tggtgactga atcactgctt
    15961 ccccggttag agccaaactg gctgagtgtg ttccaggcac tggcaatact gctgctgaaa
    16021 ccatgcaggg cactttccgc cttattcatc gactcccggg ccatttcctg ttgcgctgct
    16081 gcaatctgac gcgttgcatt aataccaacc ggcaaacggg acattgcccc actggcgtcc
    16141 atcaccatac tgccgtcacg ggtctgtgtg gcggttgcgc cactgccagt ctggtacatc
    16201 atctgaccaa acgacgtggt gctgttggtg ctccagctga agccgttcac gttttccgtc
    16261 tgcatgttgc cgtaggagta attaccgtca accacactgc ctgccgcact ggcggttggc
    16321 gtgatggaag aagacgcaaa gtggctgtat acgctggaaa atccggcacc aaggcctcgt
    16381 accatcatcc acgaaagagg ggggatcatc gctgagagat aacctgcggt tgatgccagg
    16441 tcggagtatt tcagctgtat ctgagagact tccgacaaca ccaccggggc accgttctgc
    16501 ttcgcataga atgtcatggc actgttcaga atggcgtaca gcagaggcca tgtctgcagc
    16561 cacataaggg caaacacata gcccttcagc accggcagtg tcattttatt aaatgcagca
    16621 gccagtacca gaagcgggaa aataccaatg gttattccgg tcagaatggt ctggaccatc
    16681 ggcaggtttc gcattattac atgtccgaca gagacgtgcg ccagtctctg tttctccatt
    16741 gatgaggtgg tggccagact gaccaagctg gccgtatcac cgttacgggc cgcattactg
    16801 gtgataccct ctttcagtgc atttattgtc acattcttac gcataatctg actggccgac
    16861 tggctggccc cgtagaaata gctgtaactg tcactgagca gttccctgaa taacaggtca
    16921 gggtccggtc tgccaccaaa taactgctgc acataataat gccaggtctt gcctccggtc
    16981 tttgtatcaa gattcagctt gtctttcagc gtgaccgacg catccttgca gctgacaaaa
    17041 ttattattac tgtcatatac cccccgcagc gggctgggcc gggaaaaaat caccatatag
    17101 gggtcatcag ccaccatcag gtcttccagc gtgtatttgt ggttcaggta aatatcaccc
    17161 agcacgcagt tctggacgta gtcctgaaac agattgatga tttccggatt ccgggacagg
    17221 aaatcggtgc ttttcacaat caggttcgcc ccgaacagca tgcctgtctt gctgtaggtg
    17281 gcgctgtccg gctgagcgaa aaccatctca tagccggcca ccatcgcatg cccgatacgg
    17341 gtcgtcagtg aaagtggcat cgccagcccg accggtacat tatccacacg atggacctga
    17401 accaggtcac tgttatcaat aatctgcacc gatgtgcgga cattaaccag cagactgata
    17461 agtacaaaca cggcaaccca tcccagcaaa tccatcacgt tgtgccgttg tacccacatc
    17521 acagccactg cgagaacaga taatgtgagc gcaatttttt cgatggaatc ccacgtccgg
    17581 gtactcataa aggcagcgat ggcattcagg ttgttccgta accattcccc accggcgatg
    17641 acatagacct cgttcactgt acgcctcccc cgaagtgata attattctgg taacggctga
    17701 gcatacgggc agacaactgc tggcgcatgt agctcatctg acggtcaacg accagcaatg
    17761 catcctgctg gacctgcacc tggctctgga aaatggcaat ctgttgctgc gcctgattaa
    17821 ggttatcgag cacactgtcc aggaccgcct gcgggtagtt cccggtggaa atcatcgccc
    17881 gggcctgctg aagcagttcc tgaatgtact gaagcatgat gtcgtaaccg atatagtccg
    17941 tcagctggta gagaacactg ttggatacgc ccagcatcag tgggtcaacc agatatctga
    18001 aaacaggaat ggtggtgctc tgaatgaagg ttttttcgtt ctcagccagc ggagaatcac
    18061 tgagcgcctt gttctgaata ctggtgagca gtttcgctat ctgtgacttg agcgccctgt
    18121 ccttgctgat ggtcacattc gcatcggcca ccactttgag gcacttatcc gaatcattgc
    18181 agtgataaat tttcgcggta cccccttcca taatcgctcg gacaatatcg cggtcagtgg
    18241 ttcgtgcagg aagaggggta atctcactgt cttcaccaaa aatcagcgta cctgtgagcg
    18301 tcataatgaa ctctttcagc tctttgttac catcaagcat cctgtttttg gacagcgagt
    18361 cccagatgat attgatgttt ttcagtaccc gctccttgtc cttatcgctg gccttgtcac
    18421 gcaccgcaga agactgccct ccgaccgtgc acccctgacg ggaagcggcc cagtcagcga
    18481 agatattgct ctcacctgca atatcctggc agaccttctg ctgcgactcc tgcgtctgag
    18541 gaaacaaccc gccaactatc ccctgggcca cctgacagct actgaggttc atactgttaa
    18601 tgtcattcgc catcttctgc aggaagtctt tcgcggtttt gatttcgggc accgttgtct
    18661 gcagggcaag gtcaaaaaag taccccatcg cgttactcat aatctgctta acaaaacgct
    18721 gcagctgctc gccattaata aaactgaatg agccgagata ggcatcgatg ccaccacacc
    18781 ctgcattgat gtcgggcagt gtcatggaga taagctggat atttctgacc tgattacggg
    18841 catacagaga accgccatag gcatacccgg cagcctgccc ctgccagact ccggcctggg
    18901 tggtattggt ggcaaagccc agcttgttga agaactggtt catgtcgctg ttcacatccg
    18961 ctgacgcagc aggtgtgacc gtcagtaagg cggcacaaag aataaaaaga ggcttaatgc
    19021 gaagcattat tgctgccctc ccttaatgtg ttctcccggc gcatacggcg tttcagcagg
    19081 aatatccgga cacccccaaa tattatttcg cacaacggcg acagagaagc cgcagcaaga
    19141 aaaccagctg caagcgcata taacataatc ggaatgacat acatcagggt gttccacata
    19201 taacgaaaat tatttaattc cgggtcagct ttccacgaat caataccggc actgaaaaaa
    19261 tcatgcataa cgttccttac aatcaatccg gcagcaaaga aaaccacaaa aagccagaag
    19321 tattttatgg cgttatcaca acttcgctca atatcccgtt taatttttct gattttgaat
    19381 tcctgattct ttttgttttc tttcattata agtttcctct gatatttctg atgattcatt
    19441 cagaccggtc atgcggctgt aatatatttc agacaccaaa atccatccca caattgcccc
    19501 tacaaaaaac acaacagaaa cagaaaaggt aagacacagc gaaacgacaa tttccacagg
    19561 gctggcggga taaaaaagcc cctgacggat aaaggggata ttcaggctga cgattattcc
    19621 cgtcgcgacg ccggatattc cggcaataag taacaccacc tgcttatttc gcacctgttt
    19681 tccctccgta catctgcaga acggtatcca tccgcgccat aaaaccggcg gcatccgttg
    19741 ccccctgtaa aagcggtaat gcctcaagcg tgttgacgtt gaccaggaag gtggtcggtg
    19801 tggccaccgg aatattcggg aagaaggtct gcatcacgtc cggtggcacc ggtaatgctt
    19861 ccggaaaggc ggtatccccc tgaccatcca gggtgtaggg aaaaacggaa aagccgtact
    19921 gctgtgccag ctgcttcagt accgggtcaa actggtgaca gtaagggcaa tgcccctgca
    19981 taaacagcac caccttccag tcggccaggt taacctgcct gccgttgctg aggcggaacc
    20041 agcggggagc cggcttttct gtcttgtctg tcctggctga cgtatctgct gccggttgtg
    20101 caggctgtgc ggccataccc tgcggattcc agagtcgctc tatttcatcc cgggtggagg
    20161 cctgcacggc agcggctgac agtaacaggg tgaacagcag tgatttagtg agagacatgt
    20221 ctgctctcct tttctgcagc aagaatggcg gcctgacggg cttccagtgc ctcacgctct
    20281 tcccggctga cgcgggctat ccatgtatcc agtatctggt atgcgccaaa cagaacgaga
    20341 atggccgcaa tcagctctgc cagaaaaaaa gccatccagg gctttctgta aaccagacgg
    20401 gcaacaatat gaaaccagag ccccagggag aacacccata cacctgtaat atcaaggcgg
    20461 ggtaaagaga atttatattt actcataaat atatcctgtt tgtaatatct tccacactca
    20521 gcaggatggc aaataaaaaa ccgactgatt attattccgg tatactcatt tcatttttac
    20581 agcgaatgta caaaaaacag aggagcaccc ataaaacaaa cagatcaaca tgaaatgctg
    20641 gcgtcattaa aaaattatcc agaaatctga gaagccagat acctgctgca atgattaggg
    20701 caagcacaga caagtacaat aacctgctgt tacgctcgta aagaaaataa agtattacca
    20761 ttacaacaga cagcgccaca tacattaccc cggtcacact cagaattaac acattcatca
    20821 accagctata atagcgattc atactgacat ccggctccgg aaaactcata aaagccagta
    20881 aggtcagtaa aataaccgca acaccagacg cgattaacca tctttcagga atactactat
    20941 tttctttatc catacagaat aaaaccatac ttaacaaaca acatgaatta aaagccattc
    21001 accatcaggt gtggggacag aaaaaagtta tctctgattg tcttttactc tgtaaatgag
    21061 ttcatttaca tattaacagg gcattatttc attatggttt ctgatggctg gagtaaccag
    21121 tagaatataa tcattcctgc accagaaaac actgaggcaa cagcgagcca caaacgctta
    21181 tgcacgcctt ctgatatgcc tgaaaaatat cctgtatgaa gtagcagcag caaccatgac
    21241 aacactcctg ccagacccag cagggcagga gggatatgcc acagttcata tttcataata
    21301 acaatacctt ctgccatcat catggacagg ctgcaaacca caaaacagtc ccatgaaaaa
    21361 aaacgcctgt cagcagcaag gaaaatcact gtcagtgaca tcactgtcac ctgggccagc
    21421 aataaaggcc ataaagaaat aaaattcatt taaacgacct ctttaaaaat tcggtttaaa
    21481 gtcttcagaa acgttcagga actgttttgc caggtcgtcc tgagaaataa agccgtatga
    21541 taacgggcga acactgccct gtttcgggtc aaccagcatc atggccggga aatatttcac
    21601 accgaggcgc tgcgcctgcc cctggtcagt ccgggaatcc ggcaacagtg gattaatcac
    21661 gccatccacg gaaacgggaa taacactcag accatacgta tcccggaagc cattaatgac
    21721 ctgcgccagc tgcccgtcga tggggtcctg cccccggtaa aaaaacataa tgccgtattg
    21781 ttcagccagt ttcgcaatgg cctgtcgctg ctgtgcctgg tctgccgcca gctggttccg
    21841 taccgtgccg ttgtaatggc tgtactgcag gttatagtcc agttcaggat gtgccagcat
    21901 ggcctttttg gcgctcatgg tgaaaagccc ggcctgctga gtccagtaat tctgcagccg
    21961 gaaatatttc acaaaattat ccactccggg atacagaatg gcttcgtaca gcgcccgctt
    22021 tgttgccgtc tgcagcgcgg tcagtttctg cataatatcc ggctcctgac ggggaattac
    22081 aggtgcaggt ttattttccg gttccggctg tttcatcttt tcgttatacc actgccagcc
    22141 tgcatctttc cctgacgccg gaaaaataaa gcagcagagt aacagtggca gtaatgcttt
    22201 attcatcttc attcctttca tcactttttc tttcatcagg tttctgcagg tcagtcagag
    22261 cagaaaaaca aaaatacaga ataaacagga gcacaccgga gtaaaaaata acgaaagcag
    22321 cagcaagcac cccgcctgcc gtggtttctt ttaccgtatc ccatgaccag ataattaccg
    22381 gggaaatgac aatcgctgta atcaaaagcc ggataagaaa atcaacaaga cgattcgacg
    22441 tgaatatgac tttcattatt ttcccgacct tactgcccca cctgtttcag ctgaccggca
    22501 atctgctctt tcactttttc ggtcagcgca ccattatccg ggattttctg gttattcatc
    22561 aggtcttcgt agaagttagt gaagtccagc tggtcgaact taatacgctg cagttcatcc
    22621 acggttattc cccggcagtc aggacttttt gcgctgccaa acccgatacg caactgcccg
    22681 ttacgcccct gctgctggac tatctgcgcc agcttcgaat caaactggca gtaactgcgc
    22741 tttttctgca gacagacacc cagcactttc cgggagcaga actcgcccac actcaccgtc
    22801 agtttgttgt cctttgcctt tcccagcgcc ttttcttcgc tgttgcactt cgccagtccc
    22861 acatcctgac cccagccacc atccttacag cagttactga agccggctgc ggccttacgg
    22921 cagaatttcg cctcgcccgt gaaagccctg acatccacac cattcagggc ggcaacatcc
    22981 ttaccagcag cggccagtgc cgccagctgt gatactgcct caccaaaatc actgcttttt
    23041 ccgctatgcg ccttgtcgca ttcaccgtca aggcagaaga catcaccacc acagaccatc
    23101 acttttccgg acgttctgga ctcgcaggag tacgtggcat actcatgcag acaagctccc
    23161 tcatcggaat aaaacgcgca ctgacgtgag gccagcgtac aggccggatt atcgacatat
    23221 ttctgacagg tgccgttatc tgcggactgt gtgacatatt tatcccggta tgcccagcac
    23281 gcctgtgtga tgctccaggt tttaccttcc atcacacccg ttttcgtacc acccggttct
    23341 gaacactcag tgcctttcag cacgccatcc gctttattaa acggacaact ctccacccac
    23401 tccacccgtg gaacccattc cttatcacgg accttcatcc tgagtttcag cgtaaaggtg
    23461 gaagcaccac tgacaagcga ttcatagacc atcctgtcac cattcccgtg cgggaggcaa
    23521 ttaccgtttg cagtacagcc actaccggtc agaacctgcc cctgtgtgac agaaaacccg
    23581 gagggcacag gtatggtgaa agtcccgctc tgtcctgtaa ccagttgcac attaaaggct
    23641 gtgttcataa agtcgtaacg ggagttaagg aaatatagcc ctgcatgagc cgacagcgag
    23701 gcgctttcca ctgtcccggc aacaggtacg gtaacgctga acaccagttt cagaccgttc
    23761 atactgaacc ggaactgtga gtgcggtatc gtcacctcac gatattcatc cgtgtatttc
    23821 cagtctcctg tgatcctggc tgtcctggaa caatattcct ccaccatcgt gtcacgttca
    23881 caggtatagt ttgtgaattc actgcggttt atctgctgcg cactgcactg ctgtccggta
    23941 tttcccacga tactgtcagc ccggttcacc acatccctgc cggtctgaat aaacggcgca
    24001 tccggtgaca gaatgtcttt cggcttgttc atgaatgact ctgtgatggt tttgccggtt
    24061 tcactggttg cccattgtgt ggtgccgtta tttttcagac cgccatcccc gccggcagtc
    24121 accccaccgt aatactgcgt ctcatccggg ttcgcgttat acccggggat actctcctgt
    24181 ggattaaaac tctgaatact gccaataccc tgcccctgga tctgacgggc aaaatcagcg
    24241 ccggcacggt aatcactgtt gctgtcagcc tgtgccacac tgactgccag ccccagcagc
    24301 aatggcagaa gacgtttcat acctcactct ccctctgata ttgctcaaac cagaacacga
    24361 ctggtttgtc caccacctga atcagcccga accgttctgc ggtcctgaaa ttcacgctgc
    24421 ttctgcgccc ccgttcaacc tgcagggaca cctgcgttct gaacatctcc agcgaatatg
    24481 ttgttttcag caggttgcac ggcgcacagg cggggacgag gttttccgga cagtcggcct
    24541 ccggtctgaa aacatcaccg gtggatttca gcctgaaaat gcgcttttct gcagccttca
    24601 tgcactgctc tgacttgcgg agcaccgcct gaacatggtc ggcatgccat cctttctccg
    24661 gcagttcaca gccgcagtac gcacaacggc caccgaattt catacgcagt ttttcccgct
    24721 gtttctttgt cagcgccatc aatgcctcct taacaggaac agatttacgg attcactaat
    24781 cggaccagcg tcctgaagtc tgcacgcagt gagcggcgac agtaccggtg ataccagtga
    24841 tgagggcctt caggctgaag ccggaagcaa agctgaaaat cactgttaaa agtccccttc
    24901 accgaccatc cccatgtcag acgtggtatt gctgattcag catcctgccg cagtcgctgc
    24961 tccagaaggg cagccatttt taccggacag gcaaaggcga tataaggcgg cagctttgta
    25021 acagggaaac catgcccctg acaggacgca tatgtacgaa atccgtgaac gttcatcgcg
    25081 gccaccagtg cacggatgtc cgggtcaatc cggtaatagc ctgcaggtaa actcattgcg
    25141 acttatcccc ggtgccgtcc agcagtccct gagccacctg ccggcagtct cctgtggcga
    25201 ccactttctc cagcgcctgt ctgacgcgca gatttccccg gatgatgtcg tgcccctggc
    25261 tgcagaacac caccagagcc ggcacgctgc ggatgccgta ctgtgaaaac agcgtcgggt
    25321 cgatctgaac accatcggtc gcaccgtctt tcaccagaga cagcactgct tcagccgtgg
    25381 ctttcaggtc gttgttcacc ataccccgca gtgtggccgg aataccgtag tgccgggttt
    25441 ctcccagcat ccgtttcagc ccctcttcgg gtatggaaaa cgacacaaaa tacacggcac
    25501 cctgctgtgg tttatccttc tgagcagcct gctgtttacg gaccagttca tcgagaaact
    25561 ggttatcgga atgctgaagg ggattcgccc tgacctgctg ttctgcccat gcctgcagct
    25621 gtgtgtccgg cttttcacgt aactgctgac tcagtgtttc ctgctgcttc aggaactggc
    25681 ggttttcggg tgtattcaca ttttccgaag catgggcaga aataaacagt cctgtggtca
    25741 gggcaaaaag tacaaatatt ttccggctca tatcatctcc cggcatagtg atataattta
    25801 attaccggcg cacttttctt tcagtctctc agacaaagca tataattcat ccatttcttc
    25861 ctgagtaatg cattttctgc tgacagtaat ctgcaccgta gccacggcgg tatctctgtc
    25921 cagagtaata tcatcaccgt aattttttaa tgacagatgg tctccctctc tccggacctg
    25981 gtatattttt tccggtgggt attcagacaa ccatttctcc cacctgtctt tagatttatc
    26041 attacagtta tattcagaca taatgctgat gaactcccga aatcttctgt caataaactc
    26101 cagtcggttc agtatatcct ctgatttcct gatatcttca atatctgtaa gccgacggca
    26161 ctcctgcaga agataatatg ccccaccatt actttcagcc cattgtctga ccttatcaaa
    26221 agcactcacc ttttccagca gtgagaacca attctcctca ctgataaaaa cccctttttc
    26281 tttatcactt atctttcgta aaaatgagaa aaatcccata acgcaccttt acttatattg
    26341 tacacatgca cacactgtta tatttatgat aaccatcaga ctcatttcct gcgcttaacg
    26401 ggcataaact tatctgaaga aagatatgaa ataacaacgc ccaccactgt aagaataaaa
    26461 gacgtcttca gatacagtct gacaagcccg attttttcca gtgtatctgc atcatgccag
    26521 tcattaacaa tgtctgagag aatatattca gggcaaaaat aaaaccacac cacagagcac
    26581 atcaccatag caaaaggaaa cagctttaat acattgctcg ttaaccggat aaacagaaga
    26641 catatttttc tgaagtcatc tgcgtgttta tttcttatta cccacacctt catataatcg
    26701 atcctcttat tgattaacat gacttacagg aacacacagt tacgtttacg ccacatcagg
    26761 taaccgaagt ttttcttagt gttgggcgga ttttttcccg tctcccagcg catcacactg
    26821 cgtccgaacg gatggcactg cccgctgtcc gggtacatat tcaccatctg ataacgccag
    26881 cgcgctttgg gaagtatggg cgacggatat tcataacaga cagccacatc cacgccgatg
    26941 gtgttcatta tcattccctg acggtggagc ttaaacgcca tccgttcact gaccagcagg
    27001 gatgactgca gcggactgga ttcattgctc acccagccat taaacggata catactgccc
    27061 tgcgaaccgg cacaccagaa caggatatcc agcggcttgt taaaggcact ggcaatggca
    27121 tccgccgcac aggctccctg tgctatcgga tttgcaaaaa tgaccgcctc cggattgagg
    27181 atggtggtca ggctgctgtc cgtccaggta gggtcaattt ctgacagata ggcaatatcc
    27241 aggtctccgc cttccaggca ccccaacgac gtgatgatgt tcagccagta cgtcagcggg
    27301 tatttgtacc agtgaacgtg atagaacgcc ccgttcacct gtttatcttc ttttttagcc
    27361 gtaccctgtg ccgttttccc gaattccggc agactgaacc ccagattaac catgcatccc
    27421 ggtgagcggg tgacatccgt cagtgccatc ggctcccagt aaccaatagc cagcccgata
    27481 cgtttaaaaa cagggggagg cgccgggcag aactggagcg gcattgacgg gtttgccgta
    27541 tccggcacct ttccctggct cacttttgtg ctgcccaacg agagagggaa gatgcagctc
    27601 cagcagatat ccgtgatcgg gttaacaaac cgcccttcac aggcagcgtc cgctgacgct
    27661 gcgggaacat ggccagtcag cagcaaaacc acacacaacc acatacaccg tttcatttcc
    27721 ctccctcccc gaccggaata aactccacct tcaggaaacg atcgccactg acagcactca
    27781 cccgtgccgg cacctgatca atgccgagac gctggcagat cacgccgttc tgatcaaagt
    27841 aaacacgact gtccagagcc ttctgcatct ccgggatact gccctgcacc aggataattt
    27901 tgctttccag cgtgggaggc gtctggcgct tcatccattc aatctgctca ggatcatcac
    27961 cgttgatgaa atacagtgtc tggttaaaag gcacatactg cagcgggttc atcacctccc
    28021 cctggcgggc gaagatacga ccttcgttat cccggatatc cgcagccagt ctgaccgacg
    28081 ggtcaaacag gcggctgctg tatttctccg tccgaccgat accgggcacg gcagggggcc
    28141 gcaggctgtt gcggatcacc cgcgccttaa acgcatccat tttccggccc atctctccgg
    28201 actgctccag agctgtcaga cgctgcataa tcaccgtcag catgtccggc tcctttaccg
    28261 gccacaaatc cccccaggtg ccaagatcag ctgcagccac actctgcccc cataccagca
    28321 gggcaatcag cccccggcat ctcatggttc tgccctcatt cgccgggcaa tatcctgctg
    28381 gatttcacgg gtgatatccg gtgtcccctg taccacggca ggcgacacca gaatgacagc
    28441 gtggtgcttc tgctgccacg tctgcagact ggcctccagt gccgcgttaa aacggtcagc
    28501 cagcgccttc gactgcactt ctgacagttt tttctggctg gcgctgtcaa aaaacgcatc
    28561 cactgtctgt ttcatgttga aggcgacggt taccggcgtg ttcagtctga caatggcgta
    28621 actgacggct gcgttcagta acaccattgc caggcatccc ggcaccgtcc accaccagtg
    28681 gctgcgttga cgggctgtaa catcagtcgt tttttgcgtt gtggtcatgc aacactcctg
    28741 tatttctcat gttcttccag ccaggcttcc agcgaagcca tctccggccc tgacttcttc
    28801 cacgccagct gccacaccgc ctcatgaata ctcagcccct ccttacgttt ctgctgcaca
    28861 aactcaaaat ccgggccgtc agagctgtac atggcacggc ttaacgggtc cacaaacaga
    28921 cggtgccagg aggaatggtt ttccacctgc agcaggaagg aactgaacca ctggtctttg
    28981 gccgcgccaa acttgccaat catgtcacgc tgcagtggca ggaactggtc cggatacagc
    29041 tggttgtatt tcgcgaactc cttcgcactc tgtttgagga taattttgta ggaggagtta
    29101 ccccatgccg cacgggcagc actggacgcc ttgtcagagt caaagtcgac gatgttctgt
    29161 gtgatggtga tataggcacc ggtatgacga cgggcggtac ggtaacct
//
LOCUS       DAZFRN010000050        29070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFRN010000050 DAZFRN010000000
VERSION     DAZFRN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.050"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(144..1808)
                     /gene="glnS"
                     /locus_tag="ACHKUB_002126"
     CDS             complement(144..1808)
                     /gene="glnS"
                     /locus_tag="ACHKUB_002126"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO6655146.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVADKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2011..3957)
                     /gene="nagE"
                     /locus_tag="ACHKUB_002127"
     CDS             complement(2011..3957)
                     /gene="nagE"
                     /locus_tag="ACHKUB_002127"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO6655147.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4290..5090
                     /gene="nagB"
                     /locus_tag="ACHKUB_002128"
     CDS             4290..5090
                     /gene="nagB"
                     /locus_tag="ACHKUB_002128"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6655148.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5150..6298
                     /gene="nagA"
                     /locus_tag="ACHKUB_002129"
     CDS             5150..6298
                     /gene="nagA"
                     /locus_tag="ACHKUB_002129"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6655149.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6307..7527
                     /gene="nagC"
                     /locus_tag="ACHKUB_002130"
                     /gene_synonym="nagR"
     CDS             6307..7527
                     /gene="nagC"
                     /locus_tag="ACHKUB_002130"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO6655150.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7575..8327
                     /gene="nagD"
                     /locus_tag="ACHKUB_002131"
     CDS             7575..8327
                     /gene="nagD"
                     /locus_tag="ACHKUB_002131"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO6655151.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8584..10248
                     /gene="asnB"
                     /locus_tag="ACHKUB_002132"
     CDS             8584..10248
                     /gene="asnB"
                     /locus_tag="ACHKUB_002132"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO6655152.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNSKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10629..10705
                     /locus_tag="ACHKUB_002133"
     tRNA            10629..10705
                     /locus_tag="ACHKUB_002133"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10663..10665,aa:Met,seq:cat)
     gene            10715..10799
                     /locus_tag="ACHKUB_002134"
     tRNA            10715..10799
                     /locus_tag="ACHKUB_002134"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10749..10751,aa:Leu,seq:tag)
     gene            10823..10897
                     /locus_tag="ACHKUB_002135"
     tRNA            10823..10897
                     /locus_tag="ACHKUB_002135"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10855..10857,aa:Gln,seq:ttg)
     gene            10932..11006
                     /locus_tag="ACHKUB_002136"
     tRNA            10932..11006
                     /locus_tag="ACHKUB_002136"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10964..10966,aa:Gln,seq:ttg)
     gene            11022..11098
                     /locus_tag="ACHKUB_002137"
     tRNA            11022..11098
                     /locus_tag="ACHKUB_002137"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11056..11058,aa:Met,seq:cat)
     gene            11147..11221
                     /locus_tag="ACHKUB_002138"
     tRNA            11147..11221
                     /locus_tag="ACHKUB_002138"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11179..11181,aa:Gln,seq:ctg)
     gene            11259..11333
                     /locus_tag="ACHKUB_002139"
     tRNA            11259..11333
                     /locus_tag="ACHKUB_002139"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11291..11293,aa:Gln,seq:ctg)
     gene            complement(11487..12662)
                     /gene="ubiF"
                     /locus_tag="ACHKUB_002140"
     CDS             complement(11487..12662)
                     /gene="ubiF"
                     /locus_tag="ACHKUB_002140"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO6655153.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12808..14232
                     /gene="miaB"
                     /locus_tag="ACHKUB_002141"
     CDS             12808..14232
                     /gene="miaB"
                     /locus_tag="ACHKUB_002141"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO6655154.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14385..15425
                     /locus_tag="ACHKUB_002142"
     CDS             14385..15425
                     /locus_tag="ACHKUB_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO6655155.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15422..15889
                     /gene="ybeY"
                     /locus_tag="ACHKUB_002143"
     CDS             15422..15889
                     /gene="ybeY"
                     /locus_tag="ACHKUB_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO6655156.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15979..16857
                     /gene="corC"
                     /locus_tag="ACHKUB_002144"
     CDS             15979..16857
                     /gene="corC"
                     /locus_tag="ACHKUB_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO6655157.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16882..18420
                     /gene="lnt"
                     /locus_tag="ACHKUB_002145"
     CDS             16882..18420
                     /gene="lnt"
                     /locus_tag="ACHKUB_002145"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO6655158.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18498..20375)
                     /locus_tag="ACHKUB_002146"
     CDS             complement(18498..20375)
                     /locus_tag="ACHKUB_002146"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO6655159.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWQRARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQIDESVLQVRQSLREEFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIEDTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGEGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISIGDQQTSIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20830..21738
                     /gene="gltI"
                     /locus_tag="ACHKUB_002147"
     CDS             20830..21738
                     /gene="gltI"
                     /locus_tag="ACHKUB_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO6655160.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21908..22648
                     /gene="gltJ"
                     /locus_tag="ACHKUB_002148"
     CDS             21908..22648
                     /gene="gltJ"
                     /locus_tag="ACHKUB_002148"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO6655161.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22648..23322
                     /gene="gltK"
                     /locus_tag="ACHKUB_002149"
     CDS             22648..23322
                     /gene="gltK"
                     /locus_tag="ACHKUB_002149"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO6655162.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23322..24047
                     /gene="gltL"
                     /locus_tag="ACHKUB_002150"
     CDS             23322..24047
                     /gene="gltL"
                     /locus_tag="ACHKUB_002150"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO6655163.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLHSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24165..25100
                     /gene="rihA"
                     /locus_tag="ACHKUB_002151"
     CDS             24165..25100
                     /gene="rihA"
                     /locus_tag="ACHKUB_002151"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO6655164.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25184..26854
                     /gene="hscC"
                     /locus_tag="ACHKUB_002152"
     CDS             25184..26854
                     /gene="hscC"
                     /locus_tag="ACHKUB_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO6655165.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26914..28365)
                     /gene="djlC"
                     /locus_tag="ACHKUB_002153"
     CDS             complement(26914..28365)
                     /gene="djlC"
                     /locus_tag="ACHKUB_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO6655166.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVGEADNEDKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLAELCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRLLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28362..29069)
                     /locus_tag="ACHKUB_002154"
     CDS             complement(28362..29069)
                     /locus_tag="ACHKUB_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6655167.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         7143 a         7555 c         7354 g         7018 t
ORIGIN      
        1 tttattttat ggataaattg cgaagcggaa ttaattcgcg aagaaaagca gaaaaaacgc
       61 cgcagagtgc ggcgtttggg attgtttgct atttcttgcc ggatgcgacg taaatgcctc
      121 acccggcctg gtagaactta aaattactcg cctactttcg cccaggtatc acgcagccca
      181 acggtgcggt taaataccgg tttttccgct gtggaatggc ggctgtcgag gcagaagtaa
      241 ccttcacgct caaactggaa tgctttaccc gcaaccgcat ctttcagcga cggttcagca
      301 aagccctgtt tgatcaccag cgattccggg ttaatcaccg acaggaaatc atccgcagca
      361 ccagggttcg gcacgctgaa cagacggtcg tacagacgga tttcaaccgg cagcgcatgt
      421 gccgcgctca cccagtgaat aacacctttg actttacgac catctgccgg atctttgctt
      481 aaggtgtcgg cgtcataagt acagaagatg gtggtgatat tgccttcggc atctttctcc
      541 acgcgttctg ccttaatgac gtaagcatta cgcagacgca cttctttacc cagcaccaga
      601 cgtttgtact gcttgttagc ttcttcgcgg aaatcggcgc gatcgatcca aatctcaccg
      661 ctaaacggca cctggcggct acccatttcc ggtttgttcg gatggttcgg catggtaacc
      721 atttcgcctt cgccctgata gttttcgata accagtttca ccggatcgat aaccgccatt
      781 gcgcgcggcg cattttcgtt gagatcttca cggatgcagg attccagcga tgccatctca
      841 atggtgttgt cctgcttggt cacgccgatg cgtttgcaga actcacgaat agaagcagca
      901 gtgtaaccac gacgacgcag accggaaatg gtcggcatac gcgggtcatc ccagccttca
      961 acgtgcttgt cggccaccag caggttcagc ttacgcttgg acatcacagt gtattccaga
     1021 ttcaggcgcg agaattcgta ctggcgcggg tgaacaggaa tggtgatgtt gtccagcacc
     1081 cagtcgtaca ggcgacggtt gtcctggaac tcaagcgtac acagagagtg cgtaatacct
     1141 tccagggcat cgctgatgca gtgggtgaag tcgtacatcg ggtagatgca ccacttgttg
     1201 ccagtctggt ggtgttcagc aaatttaata cggtacagca ccggatcgcg catcacgata
     1261 aacggtgaag ccatgtcgat tttcgcacgc aggcaggctt taccttcttc aaaaccaccg
     1321 gcacgcattt tttcgaacag cgccaggttc tcttcaacgc tgcggtcgcg gtacgggctg
     1381 tttttacccg gttgcgtcag ggtgccgcgg tattcgcgga tctgttccgg cgtcagttca
     1441 tcaacgtacg ccaggccttt attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa
     1501 taatcggagg agtaacggac gttaccagac cagtgaaaac ctaaccactc tacgtcgttt
     1561 ttgatcgact caacatactc gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg
     1621 ttgcactggc ctttatagtc ctgggcgatc ccgaagttca ggcagataga tttcgcatgg
     1681 ccaatatgca gatagccatt cggctccggc gggaaacggg tgtgtactgt ggtgtgctta
     1741 ccactggcca gatcttcatc gatgatctga cggataaagt tagtcgggcg ggcttctgcc
     1801 tcactcatcg tggattcctc aaagcgtaaa caacgtataa cggcgtatga tcttataagc
     1861 gggacaggct gacaactgtt agttttttaa aattgataat ctaacaagga ttatggggat
     1921 gattgatgaa aaaaaaagcg gcagagaaga tctccaccgc ttgtcattgt tggatgcgac
     1981 gctcaagcgt cgcatcaggc ataaagcaga ttactttttg atttcataca gcggtgtttg
     2041 acccgccaca acatggccct gagctttaat gatcaagcca ctgaaatcgt cgatattgct
     2101 gcaaaccacc gggctaatca tcgagcgggc gttagcgttc aggtaatcca gatccatttc
     2161 cagaatcggt tgccctgcgc ttacctgcgc accctcttcc accagacgtt taaagccttt
     2221 accttccagc gctacggtgt cgatacccat atggacgacg atctccgcgc ctttttcggt
     2281 ttccaggcag aacgcgtggt tggtgttgaa gattttcacg attgtccctg cggctggtga
     2341 tacgacgatt ttatctgtcg gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa
     2401 tgcttcgtca ggaacctgat ccagtgccac gacatcaccg gtaatcggcg ataccagctc
     2461 cgcgatagat accgcgtttg gtacagcctg cggttttgct acaggcgcgg cagttgccgg
     2521 agttgcttca gctgacgcag cggctaccgg accacgggca acgactttct tcatcgcatc
     2581 gccgatggat tctgctttcg cgccaacaat cacctgaata gtctgtttgt tcagtttcac
     2641 tacaccagaa gcacccagac gtttacacat cgtatcgttg acgcgggcag agtcagccac
     2701 ggtcaggcgc agacgggtga tacaggcgtc aattgctttc aggttgtcag tgccgccaac
     2761 cgcagcaata tagttggtcg ccagttgatt gaggccttct tcagtgttgc tgttggcttc
     2821 ttcagtaacg atctcgtctt ctttatcttc acgacccggc gttttcaggt tgaacatgcg
     2881 gataaccaaa ctgaacacca cgaagtagat agcgaagaag acaacaccca taaccagcag
     2941 catccagacg ttctggctgg cggccggcag gttatacatc aacgcgtagt cgatagcccc
     3001 cgcagagaaa gagaagccag catggatacc cagcagtgtt gccacaaaca ggctgatacc
     3061 ggtcagcagt gcgtgcagga ggtacagcag cggtgcaagg aacatgaaca ggaattccag
     3121 cggctcagtc acaccggtca ggaacgcagt aacagcaaca gaaagcagca tcccgccaac
     3181 catcggacga cgctctttcg gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc
     3241 gaacatcata atcgggaaga agccggacat gaacatcccc gcggtgccgt caccggcata
     3301 gaagcggttg atatcaccgt ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg
     3361 gaaccaggcg atggtgttca gcacctgatg cagaccggtt gggatcagca gacggttgat
     3421 gaaaccaaag ataccggaac ccagcgcgcc cgcagaaacg atccactcgc ctcctgcatg
     3481 gatagcgtgc tgtaccggcg gccagacgta accaaaaatg gccgccagca ccaggcagaa
     3541 gaagccggtg gcaatcggca caaagcgttt gccgccgaag aaactcagga agtccggcag
     3601 tttaatatcg gaccaacggt tataggctgc gccaccaacc agaccggtaa tgatacccgc
     3661 cagtacaccc atgttaattt ctgggttgat ggtcaccatc gctttggtta acacaaagta
     3721 acctaccgca cccgccagcg ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac
     3781 accgatggcg aagattaatg cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa
     3841 cgcaacgtta agtaaatctg gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac
     3901 cgcgataggg agctgtaacg ccctaccgag tcgctggaaa aaacctaaaa tattcatctt
     3961 attcccccta cgagaaccct atttggctcg tttaaagccg tatttttatt ttgctgcaaa
     4021 ttgtactgcc gatgttctgt aatcagattg ttagatcatc tgctacagag tgtgtgaaaa
     4081 tttaattcgt atcgcaaatt aaacgcgtgt cttttgtgag ttttgtcacc aaatatcgtt
     4141 atcatcactc ccttttactg gctaaagcag aaaacttatt ttatcattca aaaaatcagg
     4201 tcggattgac gcctgtctgc gcaaatccag gttacgctta aagatgccta atccgccaac
     4261 ggcttacatt ttacttattg aggtgaataa tgagactgat ccccctgact accgctgaac
     4321 aggtcggcaa atgggctgct cgccatatcg tcaatcgtat caatgcgttc aaaccgactg
     4381 ccgatcgtcc gtttgttctg ggcctgccga ctggcggcac gccgatgacc acctataaag
     4441 cgttagtcga aatgcataaa gcaggccagg tcagctttaa gcacgttgtc accttcaaca
     4501 tggacgaata tgtcggtctg ccgaaagagc atccggaaag ctactacagc tttatgcacc
     4561 gtaatttctt cgatcacgtt gatattccag cagaaaacat caaccttctc aacggcaacg
     4621 ccccggatat cgacgccgaa tgccgccagt atgaagaaaa aatccgttct tacggaaaaa
     4681 ttcatctgtt tatgggcggt gtaggtaacg acggtcatat tgcatttaac gaaccggcgt
     4741 cttctctggc ttctcgtact cgtatcaaaa ccttgactca tgacactcgc gtcgcgaact
     4801 ctcgtttctt tgataacgat gtcaatcagg tgccaaaata tgccctgact gtcggtgttg
     4861 gtacattgct ggatgccgaa gaagtgatga ttctggtgct gggtagccag aaagcactgg
     4921 cactgcaggc tgcagttgaa ggttgcgtga accacatgtg gaccatcagc tgtctgcaac
     4981 tgcatccaaa agcgatcatg gtgtgcgatg aaccttccac catggagctg aaagttaaga
     5041 ctttaagata tttcaatgaa ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc
     5101 tgccctggct ccttgctcag ggcaatattt tttaaaatcg ggggtcagaa tgtatgcatt
     5161 aacccagggc cggatcttta ccggccacga atttcttgat gaccacgcgg ttgttatcgc
     5221 tgatggcctg attaaaagcg tctgtccggt agcggaactg ccgccagaga tcgaacaacg
     5281 ttcactgaac ggggccattc tctcccccgg ttttatcgat gtgcagttaa acggctgcgg
     5341 cggcgtacag tttaacgaca ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca
     5401 gaaagccaat gagaaatcag gctgtactaa ctatctgccg acgcttatca ccaccagcga
     5461 tgagctgatg aaacagggcg tgcgcgttat gcgcgagtac ctggcaagac atccgaatca
     5521 ggcgttaggt ctgcatctgg aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa
     5581 tccgaatttt gtgcgtaagc ctgatgccgc gctggtcgat ttcctttgtg aaaacgccga
     5641 cgtcattacc aaagtgaccc tggcaccgga aatggttcct gcggaagtca tcagcaaact
     5701 ggcaaatgcc gggattgtgg tttctgccgg tcactccaac gcgacgttga aagaagcaaa
     5761 agccggtttc cgcgcgggga ttacctttgc cacccatctg tacaacgcga tgccgtatat
     5821 taccggtcgt gaaccgggcc tggcgggcgc gatcctcgac gaagctgaca tttattgcgg
     5881 tattatcgct gatggcctgc atgttgatta cgccaacatt cgtaacgcta aacgccttaa
     5941 aggcgacaaa ctgtgtctgg ttaccgacgc caccgcgcca gcaggtgcca acattgaaca
     6001 gttcattttt gcgggtaaaa caatatacta ccgtaacgga ctttgtgtgg atgagaacgg
     6061 tacgttaagc ggttcatcct taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg
     6121 cggtatcgca ctggatgaag tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg
     6181 cgttgaaaaa cgtctcggca cgctcgccgc aggtaaagta gccaacctga ccgcattcac
     6241 acctgatttt aaaatcacca agaccatcgt taacggtaac gaggtcgtaa ctcaataaga
     6301 gaaagtatga caccaggcgg acaagctcag ataggtaatg ttgatctcgt aaaacagctt
     6361 aacagcgcgg cggtttatcg cctgattgac cagtacgggc caatctcgcg gattcagatt
     6421 gccgagcaaa gccagcttgc ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa
     6481 cgcgggctga tcaaagaagt tgatcagcag gcctccaccg ggggccgccg cgctatctcc
     6541 atcgtcaccg aaacccgcaa tttccacgca atcggcgtac ggcttggtcg tcatgacgcc
     6601 accatcactc tgtttgatct cagcagcaaa gtgctggcag aagaacatta cccgctgccg
     6661 gaacgtaccc agcagacgct ggaacatgcc ctgctgaatg ccattgctca gtttattgat
     6721 agctaccagc gcaagctgcg cgagctgatc gcgatttcgg tgatcctgcc agggcttgtt
     6781 gacccggaca gcggcaaaat tcattacatg ccgcatattc aggtggaaaa ctgggggctg
     6841 gtagaagcac tggaagagcg ttttaaagtg acctgttttg tcggccacga tatccgtagt
     6901 ctggcgctgg cagagcacta cttcggtgca agtcaggatt gcgaagactc cattttagtg
     6961 cgtgtccatc gcggaaccgg ggccgggatt atctctaacg ggcgcatttt tatcggccgc
     7021 aacggtaacg tcggtgaaat tggccatatt caggtcgaac cgctgggtga acgctgccac
     7081 tgcggcaact ttggctgcct ggaaactatc gctgccaacg ctgctattga acaacgggtg
     7141 ttgaatctgt taaagcaggg ctaccagagc cgcgtgccgc tggacgactg caccatcaaa
     7201 actatctgca aagccgcgaa caaaggcgat agcctggcgt cggaagtgat tgagtatgtc
     7261 ggtcgtcatc tgggcaaaac catcgccatt gcgatcaact tatttaatcc gcaaaaaatt
     7321 gttattgccg gtgaaatcac cgaagccgat aaagtgctgc tccctgctat tgaaagctgc
     7381 attaataccc aggcgctgaa ggcgtttcgt actaatctgc cggtggtacg ttctgagctg
     7441 gatcaccgct cggcaatcgg cgcttttgcg ctggtaaaac gcgccatgct caacggtatt
     7501 ttgctccagc atttgctgga aaattaatgt gcttttatag tggcgcttat tgttgtcaat
     7561 attctgggta gtccatgacc attaaaaatg taatttgcga tatcgacggc gtgctgatgc
     7621 acgataacgt cgccgtaccg ggtgcagcgg aatttttgca cgggattatg gataaaggcc
     7681 tgccgctggt gttgctgacc aactatcctt cgcagaccgg gcaggatctg gcgaaccgct
     7741 ttgccactgc cggtgtcgat gtgccggata gcgtgtttta tacctctgcg atggcgaccg
     7801 ccgatttcct gcgtcgccag gaaggcaaga aagcgtatgt ggtgggcgaa ggcgcactga
     7861 ttcatgaact gtacaaagcc ggtttcacta ttaccgatgt gaaccctgat tttgtgattg
     7921 ttggcgaaac gcgttcctac aactgggaca tgatgcataa agcggcctat ttcgtcgcta
     7981 acggtgcacg ctttatcgcc accaacccgg atactcacgg gcgcggtttt tatccggcct
     8041 gtggcgcgtt gtgtgcaggg attgagaaaa tctccgggcg caaaccgttc tatgttggta
     8101 agcccagccc gtggatcatc cgcgcggcat taaacaaaat gcaggcgcat tcggaagaaa
     8161 cggtgattgt cggcgataac ttgcgcaccg acattctggc gggcttccag gcgggtctgg
     8221 agacgattct ggtgctttct ggtgtttcat cgctcgacga tatcgacagt atgccgttcc
     8281 gcccaagctg gatttacccg tcggtcgctg aaatcgacgt tatctgaata gcaaccacgt
     8341 ctcagggcgt ggtttcttat ttttacccat cattaaaatc atcccattca ataaaactcg
     8401 ccaaaaatta tcgatccatc aataaataca tcaccacacc ctcccctttt tcacaaatca
     8461 gcaatcacca ttacgttttt tatttttgca ctgccaaatc gcttgcgcac ggtgctgctt
     8521 tgcggcattt ttttaaacaa gcaaacacaa caagcaacaa ataccaggtt aacggagaag
     8581 gttatgtgtt caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgtaag
     8641 aaagcactcg agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat
     8701 gccagcgata acgccattct cgcccacgaa cgtctgtcaa ttgttgacgt taacgcgggg
     8761 gcgcaacctc tctacaacca acaaaaaact catgtgctgg cggtaaacgg tgaaatctac
     8821 aaccaccagg cactgcgcgc cgaatatggc gatcgttatc agttccagac cggatctgac
     8881 tgcgaagtga tcctcgcgct gtatcaggaa aaagggccgg aatttctcga cgacttgcag
     8941 ggcatgtttg cctttgccct gtacgacagc gaaaaagatg catatctgat tggtcgcgac
     9001 catctgggga tcatcccact gtatatgggc tatgacgagc acggtcagct gtatgtggcc
     9061 tcagaaatga aagccctggt gccagtttgc cgcacgatta aagagttccc ggcggggagc
     9121 tatttgtgga gtcaggacgg cgaaatccgt tcttattatc atcgcgactg gttcgacttt
     9181 gatgcagtga aagataacgt gaccgacaaa aacgagctgc gtcaggcact ggaagattcc
     9241 gttaaaagcc atctgatgtc tgacgttcct tacggtgtgc tgctctcggg tggtctggat
     9301 tcctcaatta tttccgcgat caccaagaaa tacgccgccc gtcgcgtgga agatcaggaa
     9361 cgctctgaag cctggtggcc gcaattacac tcctttgctg taggtctgcc gggttcaccg
     9421 gatctgaaag cagcccagga agtggcaaac catctgggca cggtgcatca cgaaattcac
     9481 ttcactgtac aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacctat
     9541 gatgtgacaa ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggca
     9601 atgggcatta aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg cggttatctt
     9661 tacttccaca aagcaccgaa tgccaaagaa ctgcatgaag agacggtgcg taaactgctg
     9721 gccctgcata tgtatgactg cgcgcgcgcc aacaaagcga tgtcagcctg gggcgtggaa
     9781 gcacgcgttc cgttcctcga caaaaaattc cttgatgtgg cgatgcgcat taacccgcag
     9841 gataaaatgt gcggtaacag caaaatggaa aaacacatcc tgcgtgaatg ttttgagtca
     9901 tacctgcccg caagcgtggc ctggcggcag aaagagcagt tctccgatgg cgtcggttac
     9961 agttggatcg acaccctgaa agaagtggcg gcgcagcagg tttctgatca gcaactggaa
    10021 actgcccgct tccgcttccc gtacaacacg ccaacctcaa aagaagcgta tctgtaccgg
    10081 gagatctttg aagaactgtt cccgcttccg agcgccgctg agtgcgtgcc tggcggtcct
    10141 tccgtcgcgt gttcttccgc taaagcgatt gagtgggatg aggcgttcaa gaaaatggac
    10201 gatccgtctg gtcgtgcggt tggcgttcac cagtcggcgt ataagtaaga aatgcgaaac
    10261 agccccggtg aaatccgggg ctgtttcagt tattgaggtg cttgttaatt ctgatatgca
    10321 gtaaggaaat gttccgggtt tgccacatca ctgtttgaca actcatcact gtaggccagt
    10381 atttcataga ttaatttccc accactattg ttgaattccg aatttcatcc tgtacgacaa
    10441 aagaatggct gtagggtggg gaaaaaacac aaagtaaaca ataattgacg aatatagcgc
    10501 cacgctgttc gcaacctaac caaacagtca ctttcgagca attttccttg aaaaagaggt
    10561 tgacgctgca aggctctata cgcataatgc gccccgcaac gccgataagg tatcgcgaaa
    10621 aaaaagatgg ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca
    10681 ggttcgaatc ccgtcgtagc caccatcttt ttttgcggga gtggcgaaat tggtagacgc
    10741 accagattta ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat
    10801 tcaccagaaa gcgttgtacg gatggggtat cgccaagcgg taaggcaccg gtttttgata
    10861 ccggcattcc ctggttcgaa tccaggtacc ccagccatct tcttcgagta agcggttcac
    10921 cgcccggtta ttggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc
    10981 tggttcgaat ccaggtaccc cagccatcga agaaacaatc tggctacgta gctcagttgg
    11041 ttagagcaca tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccaaa
    11101 ttctgaatgt gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg gtatcgccaa
    11161 gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc
    11221 aatttattca agacgcttac atcgtaagtg cgcccagttg gggtatcgcc aagcggtaag
    11281 gcaccggatt ctgattccgg cattccgagg ttcgaatcct cgtaccccag ccacattaaa
    11341 aaagctcgct tcggcgagct ttttgctttt ctgcgttcat tcaatgtcga atgcgatgtt
    11401 gacacgtctt atccttcaat gtcggatgcg acgctgccgc gtcttatccg acctacggtt
    11461 ggcacgcatc cggcaatgtt gtaaggctac aaccctaacg catatttcag cgcctgacgt
    11521 ttcaacacgc cagcacgctc tgccgccatt aacccgagat tacgcacaaa acgcagcggc
    11581 ggcagattat tactgaatcc ggcataaaac agatccatac cactttgcat aatgaagtta
    11641 tccgccatgc gccgcatctg gtaacgcttg aggacaggat aactggccca cgcttcaccg
    11701 tagctgcggg cattcaccag aacatcaatc agggcatcga catcacgata accaagattc
    11761 accccctgcc ccgccagcgg atggatggta tgcgcggcat cgcccaccag cgcaagcccc
    11821 ggctgcacat actgcaacgc atgtcggcgc gtcagcggaa acgcaccagc ggcaagcggt
    11881 gtaacgtaac ccagacgcga cgggaaatgc ttcgcgattt ccgcctggag ttgcgccata
    11941 ttcatattct gcaactggcg aatacgtacc ggagagtcat accacaccag cgatgcccag
    12001 ttatcaaaca acggcagaaa cgcacgcggt ccgtccgggg taaattgctg ccaggtgctg
    12061 tcgccgggat cgttctcaca ctgtacgcta atcaacatac acgactgcgc atactgccag
    12121 gcatgaacgc caattcccgc catctgccgc acctgcgaat ttgcgccgtc agcaccaatc
    12181 accagcttcg cgcgaatcac ttcaccgccc ttcagttcca gctcctgaag atcattatct
    12241 cgatgtaatg caatcagcga gcctggcaca cgtaacgtta ctttcggatg agcttccagc
    12301 gcctgccaca atgcctgttg caggacggtg ttttccacca tatagccaag cagcggcagc
    12361 ttaagttcag cggcgtcaaa catcacatgc gccgtttccc actcccacgt ttccagtctg
    12421 cggtaaggat ggcaacgcat agcctgtact gcatcccaga cccctaaccc tttaagcaat
    12481 gataccgatg cggcgctgat cgccgagatc cgcacgtccg gttggctatc agcgacaaac
    12541 ggtgcaggtt ctgcgtgctc gatcaccgtt accgaaaatc cgtgctgtgc cagccccagc
    12601 gccagtgcgc cgccgaccat tcctccgccg acaatggcaa tttccgttgg ttgatttgtc
    12661 atggtcaatc atcctgttag caatatgctt taagtttaca ggatttttac ccccaagggc
    12721 tgataacgct catactggtc acaacggcag caaagcatta cactatgcgc cctgcattcc
    12781 tggctactat ttcgcaagag caagtcgatg accaaaaaac tccatattaa aacctggggc
    12841 tgtcagatga acgagtacga ttcatcgaag atggccgatc tgctggatgc cacccacggc
    12901 tatcaactga ccgacgtggc ggaagaagcg gatgtgctgc tgctgaacac ctgctcaatc
    12961 cgcgagaagg ctcaggaaaa agtcttccat cagttgggtc gctggaaact gttaaaagag
    13021 aagaatccag acctgattat cggcgtcggt ggctgcgtgg catcgcaaga aggcgagcac
    13081 attcgccagc gcgcccacta tgtcgatatt atttttgggc cgcaaacgct gcaccgtctg
    13141 ccggagatga tcaactccgt gcgcggcgac cgcagcccgg ttgtagatat cagcttcccg
    13201 gaaatcgaga agtttgaccg tctaccggaa ccgcgcgccg aagggccgac cgcgtttgtc
    13261 tccatcatgg aaggctgcaa taaatattgt acctactgcg tggtgcctta cacccgtggt
    13321 gaagaggtaa gccgtccgtc tgacgatatt ctgtttgaga ttgcccagct tgctgctcag
    13381 ggcgtgcgtg aagtcaacct gctcggtcag aacgtgaacg cctggcgtgg tgaaaactac
    13441 gacggcacca ccggatcgtt tgccgatctg ctgcgtctgg ttgctgcgat cgacgggatc
    13501 gatcgtattc gctttaccac cagccatccg atcgaattca ccgacgatat catcgaagtg
    13561 taccgcgaca cgcctgagct ggtgagcttc ctgcatctgc cggtacaaag cggttccgat
    13621 cgcattctga acctgatggg ccgtactcac acggcgctgg agtacaaagc gatcatccgt
    13681 aaactgcgtg cggcgcgtcc ggatattcag atcagttctg acttcattgt tggcttccct
    13741 ggcgaaacca ccgaagactt cgagaaaacg atgaagctga ttgccgacgt caatttcgac
    13801 atgagctaca gctttatctt ctctgcacgt ccggggacgc ccgccgccga tatggtggat
    13861 gatgttccgg aagaagagaa gaagcagcgt ctgtatattc tgcaagagcg cattaatcag
    13921 caagcgatgg cgtggagccg ccggatgctc ggcaccaccc agcgtattct ggtagaaggg
    13981 acttcgcgta agagcatcat ggagctttcc gggcgtaccg aaaataaccg cgtggtcaac
    14041 ttcgagggca caccggatat gatcggtaaa ttcgtcgatg tagaaattac cgacgtctac
    14101 ccgaactctc tgcgcggtaa agtggtgcgt actgaagatg aaatgggtct gcgcgtggca
    14161 gaaacaccgg aatcggtgat tgcccgtacc cgcaaagaaa acgaccttgg cgtgggttat
    14221 tatcagccgt aattctcagg cccgccgttc cggtgggcct tgttttttct ctctttatcc
    14281 ccatctttat cgcaatgctt gcgccttttt cctgtggcgt gaataatttc cttataggcc
    14341 ggtgaatgtt cagcttccgg cgacatacag ataagaggaa cggtttgaac atagacactc
    14401 gcgaaatcac cctggagcca gcagacaacg cgcgtctgtt gagcctgtgc ggcccgtttg
    14461 atgacaacat caagcagctc gaacgccgtc tcggcatcga gatcaatcgc cgcgacaacc
    14521 actttaaact gaccggccgt ccgatttgcg tcaccgctgc ggcagacatt ctgcgtagcc
    14581 tgtatgtcga tacagccccg atgcgcggtc agattcagga tatcgaaccg gaacagatcc
    14641 accttgcgat taaagaagcg cgagtactgg agcaaagtgc ggagagcgtg ccggagtacg
    14701 gcaaagcggt caatatcaaa accaaacgcg gcgtaattaa gccgcgcacg ccaaaccagg
    14761 cgcagtacat cgccaatatt ctcgaccatg acattacctt cggcgttggc ccggcgggta
    14821 cgggtaaaac ctacctggca gtggctgcgg cagttgatgc cctggagcgt caggagattc
    14881 gccgtattct gctgactcgt ccggcggtcg aagccggtga gaaactgggc ttcctgcctg
    14941 gcgatttaag ccagaaagta gacccgtatc tgcgcccgct gtacgacgcg ctgtttgaaa
    15001 tgctgggctt tgagaaagtt gagaaactga ttgagcgcaa cgttattgaa gtcgcgccgc
    15061 tggcctatat gcgtggtcgt acgctgaacg acgcgtttat cattctcgat gagagccaga
    15121 acaccaccat cgaacagatg aagatgttcc tgacccgtat cggttttaac tcaaaagcgg
    15181 ttatcaccgg cgacgtcaca cagatcgacc tgccgcgtaa tactaaatca ggcttacgtc
    15241 acgccatcga agtgctggcc gatgtcgaag agatcagctt taacttcttc cacagcgaag
    15301 acgtggttcg tcaccctgtg gtggcgcgta tcgttaacgc ctatgaagcc tgggaagaag
    15361 ccgaacaaaa acgaaaagcg gcgctggcgg cagaacgcaa gcgcgaagaa caggaacaaa
    15421 aatgagtcag gtgatcctcg atttacaact ggcatgtgaa gataattccg ggttaccgga
    15481 agagagccag tttcaaacat ggctgaatgc ggtgatcccg cagtttcagg aagaatcgga
    15541 agtgactatt cgcgtggtcg ataccgccga aagccacagt ctgaatctga cctatcgcgg
    15601 taaggataag ccgaccaacg tgctctcctt cccgtttgaa gtgccgcctg gcatggaaat
    15661 gtcgctactg ggcgatctgg ttatctgccg tcaggtggtt gagaaggaag ctcaggagca
    15721 aggcaaacca ctggaggcgc actgggcgca tatggtggtg cacggcagtc tgcatttgtt
    15781 aggttacgat cacatcgaag atgacgaagc agaagaaatg gaagccctcg aaacagagat
    15841 tatgcttgct ctgggctatg aggatccgta cattgccgag aaagaataat ttgccaggca
    15901 tttgactggc agacgtcccc gccgttggtg attaacgacg gcgttttgat taactaacat
    15961 gagaacccat acgacgccat gagcgacgac aattcacaca gtagtgacac gataagcaac
    16021 aagaagggat ttttctccct gttactcagc caacttttcc acggtgaacc gaaaaaccgt
    16081 gacgaactgc tggcgctgat ccgtgattcc gggcagaacg accttatcga cgaagatacg
    16141 cgcgatatgc tcgaaggggt gatggacatc gcagaccaac gcgttcgcga catcatgatc
    16201 ccccgctccc agatgattac cctgaaacgc aaccagacgc tggacgaatg ccttgatgtc
    16261 atcatcgagt ccgcccactc acgtttcccg gtgattagcg aagacaaaga tcacattgaa
    16321 gggattctga tggcgaaaga cttgctgccg tttatgcgca gcgatgctga agccttcagc
    16381 atggacaaag tgttacgtca ggcggttgtc gttcctgaaa gtaagcgcgt agaccggatg
    16441 ctgaaagagt ttcgctctca gcgttaccac atggcgatcg ttattgacga attcggtggg
    16501 gtttccggtc tggtgaccat tgaagacatc ctggaactaa ttgttggtga gattgaagac
    16561 gagtatgacg aagaagatga tatcgacttc cgtcagctga gtcgtcatac ctggaccgtg
    16621 cgcgcactgg cttccattga agacttcaac gaagcgttcg gcacccactt tagcgatgaa
    16681 gaggttgaca ctatcggtgg tctggtgatg caggcatttg ggcatcttcc ggcgcgtggc
    16741 gaaactatcg acatcgacgg ttaccagttc aaagtggcga tggccgacag tcggcgtatt
    16801 attcaggttc atgtcaaaat cccggatgac tcaccccagc cgaagctgga tgaataaaac
    16861 cgaaactgga tagataacta catggctttt gcctcattaa ttgaacgcca gcgcattcgc
    16921 ctgctgctgg cgttattatt cggtgcctgc ggaacgctgg ccttctctcc ttacgatgtc
    16981 tggcctgcgg cgattgtttc gctgatgggg cttcaggcgc tgacctttaa ccgccgcccg
    17041 ctccagtctg ccgctattgg cttctgctgg ggatttggcc tctttggcag cggtattaac
    17101 tgggtctatg tcagcatcgc gacctttggc gggatgcctg gcccggttaa catcttcctg
    17161 gtggtgctgc tggcggcgta tttgtcgctg tataccggac tgtttgctgg cgtgctgtcg
    17221 cgcctgtggc cgaaaaccac ctggctgcgc gtggcgattg ccgcccctgc tctctggcaa
    17281 gtgaccgagt ttctgcgcgg ttgggtgctg actggcttcc cgtggttaca gttcggctat
    17341 agccagattg atggcccgtt aaaagggctg gcaccaataa tgggcgtgga agccattaac
    17401 ttcctgctga tgatggttag cggcctgctg gcactggcgt tagtcaaacg caactggcgt
    17461 ccgctggtgg tggccgtcgt gctgtttgcc cttcccttcc cgctgcgtta catccaatgg
    17521 tttaccccgc aaccggagaa aaccattcag gtttcgatgg ttcagggcga tattccgcaa
    17581 tcactgaaat gggacggaga ccagcttctt aatacgctga agatttacta caacgcaacg
    17641 gcaccgctgc tgggcaaatc atcgttgatt atctggccgg agtcggcgat aaccgatctg
    17701 gaaattaatc agcaaccgtt cctcaaagca ctggacggtg agttgcgtga taaaggtagc
    17761 tcgctcgtaa ccgggattgt cgacgcgcgt ctcaataagc agaaccgcta cgatacctac
    17821 aacaccatca tcacactggg taaaggtgca ccgtacagct acgaatcagc cgatcgctat
    17881 aacaaaaacc atctggtgcc gtttggcgag tttgtcccgc tggagtcgat tctgcgtccg
    17941 ttagcaccgt tctttgatct gccgatgtcg tcgttcagcc gtgggccata tatccagccg
    18001 ccgctgtcgg caaatggtat tgagcttact gcggctattt gctacgagat cattctcggc
    18061 gagcaagtgc gcgataactt ccgcccggat accgactatc tgctgactat ctccaacgat
    18121 gcgtggtttg gtaagtctat tggtccatgg cagcacttcc agatggcgcg aatgcgtgcg
    18181 ctggagctgg cgcgcccact gttgcgcagc accaacaacg gcattacggc ggtgattggc
    18241 ccgcagggtg agattcaggc gatgatcccg cagttcaccc gcgaggtgtt gaccactaat
    18301 gtgacaccga ccaccggact cacaccgtat gcacgtaccg gcaactggcc gctgtgggtg
    18361 ctgacggcgc tgtttggttt tgccgctgtg ctgatgagtc tgcgagcacg taaacgttga
    18421 tgtgcctgat gtatcctgca tgagcaaaac gtactttctc attaagcttt ggggcatggt
    18481 tatcatgccc ctttttctta cggttcaggg atacaagcca cattgccata ttggggatga
    18541 cgaataattg cctgcggatc aagcgagcgc gtcctgtcca tttggatcaa cccgttcttc
    18601 gccatgacaa atatttttct accatccgct gaaaaacgaa ggtgattaac gtagctggca
    18661 ggataaacgc caataatctg cccggcatta atattgagca ggatgacata accttgcagt
    18721 ccatatgaca ccaacgctaa atattgaccg tccgggctaa taccctcaac ggaataaacg
    18781 ctcctgctac accattgcgt cataatgctg gtttgttgat cccctatgct tatacgtgaa
    18841 atcccctgac tatcggcaaa aataacctcg ccgttttcag gtcgaaaacg catcgccgat
    18901 atcgttccac gcgcatcacc tgcgggaaag tcttctttct gatgcgtgat ggtattgatc
    18961 cgcacgatgt tacctctgcc ttctcccgga accagtaatg ttctttcatc cggggaaagt
    19021 gccataaccc aggcgcctgg tttaccgctc gtccccgtcc cgtcgtattt ctccagcggg
    19081 atatccgcca catgctgata agccaccaga tcaatggctg acacactatt actggtgcga
    19141 ttcatcaccc aggcttttgt gccatcacgc gagaggataa gactgtcagg atgtgcaccg
    19201 gtggtaatcg actgaataat ctcccctgtc gccacgttaa tgaccgataa cgcattcgct
    19261 tcaaatgaag tcgcataaat caacttttca tccgggctta atgccagcga tctgacgccc
    19321 tgatctttac agccaccaca atgtttgact gacttttctt tcgctatggg cgctgtaaag
    19381 gtatgcagga ttttcttttg tccgaggtcg aaaacaacaa tgctgttggt gtcttcaatt
    19441 gcggcataca acgtattacc atcccgactg atcgccatat catggatatc ggcaatctca
    19501 gcgagcaaat caccactggc ctgttcacga cttaccggag cggataatgt ttccctgagg
    19561 gcgttgcgtt cctccgctaa ctgttgtttt ttttgtcgtc gctcttgttc aatctcctgc
    19621 ggataaaact cttcacgcag gctttgcctg acttgtaaaa ctgactcatc aatctgctgc
    19681 tgcgcaagcc agatagcccc ggtcagaaga agactacccg caacaataat cccaccttcc
    19741 gtaacaagcc ggttttgccc agataaacgc gctctctgcc aacccaacgc gccacccgca
    19801 atcaggccgc caatgtggca agcgttatct attcctgttt gccgggcacc gtacaacagt
    19861 gtcagcgcca ccatacccaa cagaggaaat actcgccgct ggtttttatg gtatgtgccc
    19921 gtacctgaag caaactgtgt tgctatcgct gcaccagcta ttcccagaat cgccccggat
    19981 gccccaacgc tgatgactat ctgctcactt tcgcgtaacg cataacaggc gctgatgagc
    20041 ccaccaaaga cgccggagaa taaccaggta atcaggagtc gccatttccc cagaatctgt
    20101 tccgccacgc ctcccacgac caggaatgca aacatattca tcagcagatg agaaacacta
    20161 ctgtgcagaa agatactgct caataagcgc cagggttcac ccgacaaagt aagaggcgca
    20221 acgtttgcgc cgagatggat aagtgcggat tgatcgacct cttcataact catccattgt
    20281 gcgagataac aaaatatccc aatacagagt gcaaaaagga taagggttat tgcgggtaat
    20341 tgaacattta acggcttaac cgaagatgca gacataaggt gtttcttcct tgaaaagaca
    20401 ggtaaaaagc atttttcccg tgtgcagtta tcatcccggc aaacgtccca tgccgaaaag
    20461 ataattctgt ccgaaatatg actttttcag cctaaaatta caatcgtaat taattcgccc
    20521 ctaagtattt tttggcatgt aagttgcttt atttactgtg taagcgatga aatcagccgc
    20581 gacgcaacca ggttgcacca ccacaaatca gcggtagcac caaaaaggtg cggtttaaga
    20641 attttgcttc ggaatggtgc ggcacatctc gcaaaaacaa acaataaaac agcaatatct
    20701 ttacatttag ccaaactaaa tgttacctaa caggcacaac actgcacaat aaagttgcag
    20761 acgataacaa cacaaacact cacaacgggt atccatgcgt tcttaacgca gaagataaag
    20821 gagttggata tgcaattacg taaacctgcc acagcaatcc tcgccctggc gctttccgca
    20881 ggactggcac aggcagatga cgccgccccg gcagcaggca gtacgctgga caaaatcgcc
    20941 aaaaacggtg tgattgtcgt cggtcaccgt gaatcttcag tacctttctc ttattacgac
    21001 aatcagcaaa aagtggtggg ttactcgcag gattactcca acgccattgt tgaagcagtg
    21061 aaaaagaaac tcaacaaacc ggacttgcag gtaaaactga ttccgattac ctcacaaaac
    21121 cgtattccac tgctgcaaaa cggcactttc gattttgaat gtggttctac caccaacaac
    21181 gtcgaacgcc aaaaacaggc ggctttctct gatactattt tcgtggtcgg tacgcgcctg
    21241 ctgaccaaaa agggcggcga tatcaaagat tttgccgacc tgaaagacaa agccgtagtc
    21301 gtcacttccg gcactacctc tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg
    21361 aatatgcgca tcatcagcgc caaagatcac ggtgactctt tccgcaccct ggaaagcggt
    21421 cgtgccgttg cctttatgat ggatgatgcc ctgctggccg gtgaacgtgc gaaagcgaag
    21481 aaaccagaca actgggaaat cgtcggcaag ccgcagtctc aggaagccta cggttgcatg
    21541 ttgcgtaaag acgatccgca gttcaaaaag ctgatggatg acaccatcgc tcaggtgcag
    21601 acctccggtg aagcggaaaa atggtttgat aaatggttca aaaatccaat tccgccgaaa
    21661 aacctgaaca tgaatttcga actgtcagac gaaatgaaag cactgttcaa agaaccgaat
    21721 gacaaggcac taaactaatt acaagaacca ggggcggaaa ttccagccct ctcgattgtt
    21781 acgtagcacg gacagactat acgcctgatg gtcgttcccc atcgggcctg aaaaccgcaa
    21841 tacgctgggt aacaatcttc gagggtagca gttaacgctg ctaccctctt ttttctggag
    21901 tagatttatg tctatagact ggaactgggg tattttttta caacaagccc cgttcggcaa
    21961 caccacctat cttggttgga tctggagtgg ttttcaggtg acgatcgctt tgtcgatctg
    22021 cgcctggatt atcgctttcc tcgtcggttc atttttcggc attttacgta ccgttccaaa
    22081 ccgttttctc tccggtctgg gtacgttgta tgtcgaactg ttccgcaacg tgccgttgat
    22141 tgtgcaattc tttacctggt atctggtgat cccggagctg ttgccggaga aaatcggcat
    22201 gtggtttaag gccgagctgg atcccaatat tcagtttttc ctttcatcca tgctctgcct
    22261 ggggctgttt accgccgccc gtgtttgcga acaggtacgc gccgccattc agtcgttgcc
    22321 gcgtgggcaa aaaaatgccg cgctggcgat gggcctgacg ctaccgcagg cttatcgata
    22381 cgtactgctg cctaatgctt atcgcgttat cgtcccgccg atgacttccg agatgatgaa
    22441 cctggtgaaa aactccgcca tcgcctcgac tattggtctg gtggatatgg cggcgcaggc
    22501 gggtaaattg cttgattact cagcccacgc ctgggagtcg tttaccgcta ttacgctggc
    22561 gtatgtgttg attaacgcgg ttatcatgct agtaatgacg ctggttgaac gtaaagttcg
    22621 cctgcctggc aacatggggg gcaaataatg tacgagtttg actggagttc cattgtccct
    22681 tccctgccat atctgctcga cgggctggtg atcacgctga aaatcaccgt cacggcagtc
    22741 gtgattggta ttttgtgggg caccatgctg gcggtgatgc gcttgtccag ctttgcgccc
    22801 gtagcgtggt ttgccaaagc ctacgttaac gtattccgct cgattccttt agttatggtt
    22861 ttgctgtggt tttacctgat cgtgccaggt tttctgcaaa acgtgctggg attatcacca
    22921 aaaaatgata tccgtttaat ttcggcgatg gtggcgtttt cgatgtttga agcggcctac
    22981 tattcagaga ttatccgtgc tggtattcaa agtatttctc gtggtcagtc gagcgccgcg
    23041 ctggcgttgg gaatgactca ctggcagtcg atgaaactga ttattctgcc gcaggcgttc
    23101 cgcgcgatgg tgccgcttct gctcactcag ggcatcgtac tgttccagga tacctcactg
    23161 gtgtatgtgt tatctctggc cgatttcttc cgtaccgcct caaccattgg tgagcgtgat
    23221 ggtacgcagg ttgagatgat cctatttgcc ggatttgttt atttcgttat tagtcttagc
    23281 gcgtcgttgt tggtcagcta cttgaaaaga aggacagcat aatgattacc ctgaaaaatg
    23341 tttcaaaatg gtatggtcac tttcaggtgc tgaccgactg ctcaaccgaa gtgaaaaaag
    23401 gcgaagtggt ggtggtttgc ggcccgtctg gttccggcaa atcaacgctg attaaaaccg
    23461 tcaacggcct cgaaccagtg cagcaaggtg aaatcaccgt cgatggtatc gtggttaatg
    23521 acaagaaaac cgatctggca aagctgcatt cccgcgtcgg gatggtgttc cagcattttg
    23581 agttgttccc ccatttgtcg attattgaaa acctgactct ggcgcaggtg aaagtgctta
    23641 aacgcgataa agcgcccgct cgtgaaaaag cattgaaatt gctggagcgc gtgggacttt
    23701 ccgcacatgc caataaattt ccggcgcagc tttccggcgg tcagcagcag cgtgtggcaa
    23761 tcgctcgcgc gttgtgtatg gatcctattg cgatgctgtt cgacgaaccg acatcggcgc
    23821 tggatccgga gatgatcaac gaagtgctgg acgtgatggt ggaactggcg aacgaaggaa
    23881 tgaccatgat ggtggtgacc cacgaaatgg gctttgcccg caaagtggcg aatcgggtga
    23941 tctttatgga cgagggtaaa attgtcgaag actcgccgaa agacgctttc ttcgatgatc
    24001 cgaaatcgga ccgcgcgaaa gacttcctcg cgaaaatcct gcattaatca ctctggcgcg
    24061 catacccaag tgcgcgccat ctcacgcttt atcgtttcgc ccctcgtatc tgttacatcc
    24121 tggcgttacc cttgtcgcaa agaagcacac aacaaggagc aacaatggca ctgccaattc
    24181 tgttagattg cgacccaggt catgacgacg ctatcgcaat agttctcgcc ctcgcctcac
    24241 cagagcttga tgtcaaagca attacgtctt ccgccggaaa ccagacacca gaaaaaacct
    24301 tacgcaatgt tctgcgtatg ctgaccttgc ttaatcgcac cgatattccg gtagcaggcg
    24361 gcgcggtaaa accgttaatg cgtgagttga ttatcgcgga caatgtgcac ggcgaaagcg
    24421 gtctggatgg cccggcatta ccggaaccga cgttcgcacc gcaaaactgt acggcggtag
    24481 agctgatggc gaaaacgctg cgcgaaagtg cggaacctgt cacgattgtg tctaccggac
    24541 cgcaaactaa cgttgccttg ctgctcaata gccatccgga actgcatagc aaaattgccc
    24601 gtatcgtgat tatgggcggc gcaatggggc ttggtaactg gacgcctgcg gctgaattta
    24661 atatttacgt tgacccggaa gcggcagaaa ttgtcttcca gtcagggatc ccagtggtga
    24721 tggccggtct ggatgttact cacaaagcac aaatccacgt tgaagacacc gagcgtttcc
    24781 gcgcgattgg caaccctgtt tcaaccattg ttgccgaact gctggatttc ttcctcgaat
    24841 atcataaaga cgaaaaatgg ggctttgtcg gtgcaccgct gcatgaccca tgcaccatcg
    24901 cctggctgtt gaaacccgag ttgtttacca ctgttgagcg ctgggttggc gtggaaacac
    24961 aggggaaata tagccagggt atgacggttg ttgattatta ttatctgact ggcaataaac
    25021 cgaatgccac tgtaatggtc gatgttgatc gtcagggctt tgttgattta ctggctgatc
    25081 gtctgaaatt ttacgcttaa cacacgtcat tgattaagat caccacgcgt tacctgggtg
    25141 gtcttaatct gtttatctct ttttctttca agatataaat gcaatggata atgcagaact
    25201 cgccattggt atcgatctcg gcactaccaa tagtttaatt gccgtctgga aagacggtgc
    25261 cgcgcaatta attccaaata agttcggtga atatttaaca ccatccataa ttagcatgga
    25321 tgaaaataat catattttag tcggaaaacc ggctgtatca cggcgtactt cgcatccgga
    25381 taaaacggca gcgttattta aacgtgcaat gggcagtaat accaactggc ggttaggcag
    25441 cgacacattt aacgcgccag aactgtcctc tttggtatta cgctcattaa aagaagatgc
    25501 cgaagaattt ctgcaacgtc cgattaaaga tgtggtgatc tccgttccgg cttatttcag
    25561 cgatgaacaa cgcaagcata cccgtttagc agcggagtta gccgggttaa atgcggtacg
    25621 cttaattaat gaacccacag cagctgcgat ggcgtatggc ctgcatacgc aacaaaatac
    25681 ccgttcgctg gtgtttgatc tcggtggcgg cacgtttgac gttacggtgc ttgagtacgc
    25741 cacgccggtg attgaagttc acgcctccgc tggcgacaac tttcttggtg gcgaagattt
    25801 tacccatatg ctggtcgatg aggttttaaa acgcgcggat gtcgccagga ccacgcttaa
    25861 cgagagtgaa ctggcagcct tgtacgcctg tgtggaagcg gcaaaatgta gcaatcaatc
    25921 gccattgcac attcgctggc agtaccagga tgaaacgcgg gagtgcgaat tttacgagaa
    25981 cgaactggaa gatttgtggt tgccgctgct caatcgcttg cgagtgccga ttgaacaggc
    26041 gttgcgcgat gcacgtctga agccgagtca aatcgacagt ctggtgctgg ttggcggcgc
    26101 gtcacaaatg ccgctggtgc agcgaatcgc cgtgcgtctg tttggcaaat taccgtatca
    26161 aagttacgat ccgagcacca ttgtcgcgct gggcgcagca atccaggccg cctgccgctt
    26221 acgcagtgaa gatattgaag aggtaatcct cactgatatt tgcccttact cgttgggcgt
    26281 tgaagttaac cgccagggcg tttccggcat tttctcgccg attattgaac gaaacaccac
    26341 tgtgcccgtg tcgcgtgtag aaacttattc aaccatgcac ccggaacagg attcaattac
    26401 ggttaacgtc tatcagggag aaaaccacaa agttaaaaac aacattctgg tggaatcctt
    26461 cgatgtgccg ttgaagaaaa ccggggctta tcagtcgatt gatattcgct ttagttatga
    26521 tatcaacggg ttgcttgaag ttgacgtgct tctggaagac ggcagcgtta agtccagagt
    26581 gattaaccac agcccggtaa cattgagcgc gcagcagatt gaagagagtc ggacgcggtt
    26641 atccgcattg aaaatttatc cgcgcgatat gctcatcaat cgcaccttta aagccaaact
    26701 ggaagagttg tgggcgcggg cgctgggtga cgagcgagaa gagattggcc gggtgatcac
    26761 cgattttgat gcggcgctgc agtcaaacga tatggcccgc gtcgatgagg tcaggcggcg
    26821 ggcgagcgtt tatttagcca ttgagacctc ataatcacgg ctgccggatg cggcgtaaac
    26881 gctgcatccg gcatcggcgc aaaaaacaca tcgtcaacta aacaacatcg cgattttgcc
    26941 gagagcataa cctataacca gcaccgttaa actgattttg ataatgctgg taatgctctg
    27001 tggtttttca ccctcatcat ttttttcttc atggttttca ggtgttggct catcgacatc
    27061 ttcatcctcc ggctcgagtt ttactttcgt caacagcaac cgggtgataa tggcctctaa
    27121 tggatgagca tctccgcctt ccagccttgt attaagacgg gccagcatct cttgtttcgg
    27181 aaacgcttca agtactacct gttccgctac ccgatacaaa tccagtaacg gtgaatcttc
    27241 ttctgtctcg ctaccgactc tcgatttcca ccacgcccat gctttgcgtt tgaagaataa
    27301 cgactcaata aacgattgac ctaacggcga taattttccg ctatgtaaaa tttccgccca
    27361 taacagccgc gtccgggcgc tctcaccagg aacatatttc agcggttgac cttgcgcatc
    27421 taccatgctc cgcgcttcga taaccatcac taataacgcg aaataatccg ggcaatgacg
    27481 cttgctccac tgcaacaaca aattatcagc ttgtgtagat gggtaactct cccagtatgc
    27541 gcagagctca gcaagcaggc tttccccctc gccgcagtac aaacgttgtg cacactggta
    27601 ataccgggca tcttcgcttt ctggttcttc cgcttgccac tgtttggcta caggaatgag
    27661 ttccgccata cctgtttgca ccgagctgta ccagcgcaac agtttgcgat gtaactttgc
    27721 atcatcagga atgaaccacg gaccttccat cgccaaaaac gcagtgacat agtcaggatg
    27781 gtaacggcag caacgttcca gcgcgaagta atattcaaca gtctggttct gtaacgcgac
    27841 gggatattcc agaaggctaa ggaaatcgaa acaatcacca gcctgaatgg cctcaagaaa
    27901 agcctccctt tcttcctcat ccattccttc gctgtcatta aacgaatgcc agttcaaacg
    27961 ctctgccagc aaagaagcac aatttaatga aagatatcgc gcttctatag cgattgcaca
    28021 cagcggccag cgtaattgat cgacatcctc catgttccag gtatttaatt gctgaataaa
    28081 tttctgccag gcggagggct gaaaacgatc actttctgaa tccagtaatg tcctgaatgc
    28141 acgtagtatt tcatgttcag cggcggcatc tttatcttca ttatctgctt ccccgacagg
    28201 atttaccgcc agtcgcaggg cctcttcata ggcctggcgt aatgctttaa acccctgcgg
    28261 atcggtttcg ggatgacata acggtaagcg agcaagataa gcctggcgga taatgtctat
    28321 ttgcgtcgtg ctttcaattt cgaggatttg ccaacatgtt ttcataggtc gctcacatcc
    28381 ttaagcgtta ccgggcattc aggctggaca gttaatggct gccagggaag cgtggcatag
    28441 aaacgatcgc ttcccgctgc cagctcttcg aacatccgac tcccggcgct atcgtatttg
    28501 tacagggtta gcgcctcgcg tagcgaagtg ttgtcttcgc actgagaaga ttgccagtag
    28561 agacgtccta acgaataggc ggaaaaatag tgcatccagc tgtgatagta atggtgagct
    28621 cgtacataga cgcgcgattg cagccataaa ctctcttctt ccgtcagcca gttgttgagc
    28681 acaccgaccc gtgacagaaa ccccattcga acataatccc aggctttgat tcccccttct
    28741 ccacagcaca tagcggtttg ggcaacgaat tgggtataaa tcaaccctct ctccgagcca
    28801 ccagcaatta atgctttcca ctcttcaggg gaagagcgat gccatgtcag atataagcca
    28861 gctaaatcat tggcatgacc atcatcagtc atctgataaa tagtctgaat taaatcatca
    28921 cgattattta ttccccagga tgatgagaga tcgaattctt ttataccggt atacattttt
    28981 ggcgagtcat gcgcgggttt gcaaccctga ttaagtattt ccatgggcgc agaaagcgca
    29041 aaaagaagat attgttcttc cttattcatt
//
LOCUS       DAZFRN010000051        28798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFRN010000051 DAZFRN010000000
VERSION     DAZFRN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.051"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..788
                     /locus_tag="ACHKUB_002155"
     CDS             <1..788
                     /locus_tag="ACHKUB_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO6655168.1"
                     /translation="VINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENV
                     HVLAGNHETNCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDE
                     QRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYF
                     SVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMF
                     SMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            978..1091
                     /gene="yhiY"
                     /locus_tag="ACHKUB_002156"
     CDS             978..1091
                     /gene="yhiY"
                     /locus_tag="ACHKUB_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO6655169.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1154..2221
                     /gene="yhiI"
                     /locus_tag="ACHKUB_002157"
     CDS             1154..2221
                     /gene="yhiI"
                     /locus_tag="ACHKUB_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6655170.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2218..4953
                     /gene="rbbA"
                     /locus_tag="ACHKUB_002158"
     CDS             2218..4953
                     /gene="rbbA"
                     /locus_tag="ACHKUB_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO6655171.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPIRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4953..6077
                     /gene="yhhJ"
                     /locus_tag="ACHKUB_002159"
     CDS             4953..6077
                     /gene="yhhJ"
                     /locus_tag="ACHKUB_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6655172.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6181..6411
                     /locus_tag="ACHKUB_002160"
     CDS             6181..6411
                     /locus_tag="ACHKUB_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO6655173.1"
                     /translation="MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMS
                     VACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6408..6767
                     /locus_tag="ACHKUB_002161"
     CDS             6408..6767
                     /locus_tag="ACHKUB_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO6655174.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6887..7288)
                     /gene="nikR"
                     /locus_tag="ACHKUB_002162"
     CDS             complement(6887..7288)
                     /gene="nikR"
                     /locus_tag="ACHKUB_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO6655175.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7294..8100)
                     /gene="nikE"
                     /locus_tag="ACHKUB_002163"
     CDS             complement(7294..8100)
                     /gene="nikE"
                     /locus_tag="ACHKUB_002163"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO6655176.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGEKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8097..8861)
                     /gene="nikD"
                     /locus_tag="ACHKUB_002164"
     CDS             complement(8097..8861)
                     /gene="nikD"
                     /locus_tag="ACHKUB_002164"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO6655177.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8861..9694)
                     /gene="nikC"
                     /locus_tag="ACHKUB_002165"
     CDS             complement(8861..9694)
                     /gene="nikC"
                     /locus_tag="ACHKUB_002165"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO6655178.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9691..10635)
                     /gene="nikB"
                     /locus_tag="ACHKUB_002166"
     CDS             complement(9691..10635)
                     /gene="nikB"
                     /locus_tag="ACHKUB_002166"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO6655179.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10635..12209)
                     /gene="nikA"
                     /locus_tag="ACHKUB_002167"
     CDS             complement(10635..12209)
                     /gene="nikA"
                     /locus_tag="ACHKUB_002167"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6655180.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12320..12907)
                     /gene="acpT"
                     /locus_tag="ACHKUB_002168"
     CDS             complement(12320..12907)
                     /gene="acpT"
                     /locus_tag="ACHKUB_002168"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO6655181.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12962..14011)
                     /gene="yhhT"
                     /locus_tag="ACHKUB_002169"
     CDS             complement(12962..14011)
                     /gene="yhhT"
                     /locus_tag="ACHKUB_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6655182.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVKRPVAITIVVVVMLITLTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14143..15360
                     /gene="yhhS"
                     /locus_tag="ACHKUB_002170"
     CDS             14143..15360
                     /gene="yhhS"
                     /locus_tag="ACHKUB_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6655183.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLALPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15364..15921)
                     /gene="dcrB"
                     /locus_tag="ACHKUB_002171"
     CDS             complement(15364..15921)
                     /gene="dcrB"
                     /locus_tag="ACHKUB_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO6655184.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15994..16659)
                     /gene="yhhQ"
                     /locus_tag="ACHKUB_002172"
     CDS             complement(15994..16659)
                     /gene="yhhQ"
                     /locus_tag="ACHKUB_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO6655185.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16880..17125
                     /gene="tusA"
                     /locus_tag="ACHKUB_002173"
     CDS             16880..17125
                     /gene="tusA"
                     /locus_tag="ACHKUB_002173"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO6655186.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17227..19425)
                     /gene="zntA"
                     /locus_tag="ACHKUB_002174"
     CDS             complement(17227..19425)
                     /gene="zntA"
                     /locus_tag="ACHKUB_002174"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO6655187.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAAEEPQASRLKENLSLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPENATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19499..20125)
                     /gene="yhhN"
                     /locus_tag="ACHKUB_002175"
     CDS             complement(19499..20125)
                     /gene="yhhN"
                     /locus_tag="ACHKUB_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO6655188.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20266..20625
                     /gene="yhhM"
                     /locus_tag="ACHKUB_002176"
     CDS             20266..20625
                     /gene="yhhM"
                     /locus_tag="ACHKUB_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO6655189.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20628..20897)
                     /gene="yhhL"
                     /locus_tag="ACHKUB_002177"
     CDS             complement(20628..20897)
                     /gene="yhhL"
                     /locus_tag="ACHKUB_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO6655190.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20887..21483)
                     /gene="rsmD"
                     /locus_tag="ACHKUB_002178"
     CDS             complement(20887..21483)
                     /gene="rsmD"
                     /locus_tag="ACHKUB_002178"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO6655191.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21633..23129
                     /gene="ftsY"
                     /locus_tag="ACHKUB_002179"
     CDS             21633..23129
                     /gene="ftsY"
                     /locus_tag="ACHKUB_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO6655192.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23132..23800
                     /gene="ftsE"
                     /locus_tag="ACHKUB_002180"
     CDS             23132..23800
                     /gene="ftsE"
                     /locus_tag="ACHKUB_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO6655193.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23793..24851
                     /gene="ftsX"
                     /locus_tag="ACHKUB_002181"
     CDS             23793..24851
                     /gene="ftsX"
                     /locus_tag="ACHKUB_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO6655194.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25096..25950
                     /gene="rpoH"
                     /locus_tag="ACHKUB_002182"
     CDS             25096..25950
                     /gene="rpoH"
                     /locus_tag="ACHKUB_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO6655195.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26222..27325
                     /gene="livJ"
                     /locus_tag="ACHKUB_002183"
     CDS             26222..27325
                     /gene="livJ"
                     /locus_tag="ACHKUB_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO6655196.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            27475..27741
                     /gene="ataR"
                     /locus_tag="ACHKUB_002184"
     CDS             27475..27741
                     /gene="ataR"
                     /locus_tag="ACHKUB_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFO6655197.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            27748..28275
                     /gene="ataT"
                     /locus_tag="ACHKUB_002185"
     CDS             27748..28275
                     /gene="ataT"
                     /locus_tag="ACHKUB_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFO6655198.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(28272..28655)
                     /gene="panM"
                     /locus_tag="ACHKUB_002186"
     CDS             complement(28272..28655)
                     /gene="panM"
                     /locus_tag="ACHKUB_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO6655199.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6871 a         7430 c         7971 g         6526 t
ORIGIN      
        1 tagttattaa taaagacgct gtgacgccat gtacgtttct tggcgaccaa accggcgatc
       61 gatttagtaa tatctgtggc gatcaattta tcatcgattt gttaaaacgc ataatgagta
      121 tcaatgaaaa tgttcatgta ttagctggaa atcatgaaac gaattgcaat ggtaactata
      181 tgcagaactt cacgcgtatg aaaccacttg atgaggatac ctacgctggg ataaaagatt
      241 atcctgtatg tttctatgac cccaaataca aaataatggc aaatcatcat gggataactt
      301 ttgatgagca gcgaaaacgt tacattatag gaccgataac agtctctatt gatgaaatga
      361 ccaatgctct cgatccggtc gaattagcgg caattatcaa taaaaagcat catgctataa
      421 ttaatggcaa aaagtttaaa actagccggg ctatctcttg tcgatccttt aatcgttatt
      481 tttctgtttc gacagattac agacctaaac ttgaagcact attagcatgt tcacaaatgt
      541 taggtataaa tcaagttgtt gcacataacg gaaatggtgg aagagaacgc ataggcgaaa
      601 cggggacagt tttaggtctt aacgcccgtg acagtaaaca tgctggaaga atgttcagca
      661 tgcataattg ccaaattaac cctggtgcag ggcctgagat aaccactccc tggaagtctt
      721 accagcatga aaaaaacaga aatggactta tgcctttgat tagaaggcgc acaatgttac
      781 agctctgaac catagacaac atgaatatta atgttactat ctggttgaga ctgttgataa
      841 aacgtaaaaa ggaatgctct gttcctgcaa ctgaacaggc gtgaaacgct atccaacagg
      901 atggataccg ttttgtcagc cagttttata gatcacactt atgagtatgc gtgtttcatt
      961 tacggccagg aatgctgatg atgacaacat tactacccgt tttcactaag ccttccccgt
     1021 tggcgctcaa tgctctgcgc gctggtcgta tttgtcgttt ccttcttatc ccggatggaa
     1081 gaatccgctg aacgtcgttc ggcggcagtt gtaacacgcg tcgtttgctt acagggatta
     1141 tggcgggccg attatggata agagtaagcg ccatctggcg tggtgggttg tcggggcact
     1201 ggcggtggct gccgtcgtgg cgtggtggct gttgcgcccg gcgggtgtgc cggaaggctt
     1261 tgctgtcagc aatgggcgca ttgaagcgac ggaagtggat atcgccagca aaattgccgg
     1321 gcgtatcgac accattctgg tgaaagaagg ccagtttgtt cgcgaaggtg aagtgctggc
     1381 gaagatggat actcgcgtgt tgcaggaaca gcgactggaa gccatcgcgc aaatcaaaga
     1441 ggcacaaagc gccgttgccg ccgcgcaggc tttgctggag caacgacaaa gcgaaactcg
     1501 cgccgcgcag tcgctggtta atcaacgcca ggcagaactg gactccgtag caaaacgtca
     1561 tatgcgttcc cgttcactgg cccaacgagg ggctatttct gcgcaacagc tggatgacga
     1621 ccgcgccgcc gctgagagcg cccgagctgc gctggaatcg gcaaaagctc aggtttctgc
     1681 ttctaaagcc gctatagagg cggcacgtac caatatcatt caggcgcaaa cccgcgtcga
     1741 agcggcacaa gccactgaac ggcgcattgc cgcagatatc gatgacagcg aactgaaagc
     1801 cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca ggggaagtgc tggcggcagg
     1861 cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat atgaccttct tcctgccaac
     1921 cgaacaggcg ggcacgctga aactgggtgg tgaagcccgg ctgatcctcg atgccgcgcc
     1981 agatctgcgt attccggcga ccatcagttt tgtcgccagt gtcgcccagt tcacgccaaa
     2041 aaccgtcgaa accagcgatg aacggctgaa actgatgttc cgcgtcaaag cgcgtatccc
     2101 accggaatta ctccagcagc atctggaata tgtcaaaacc ggtttaccgg gcgtagcgtg
     2161 ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctc gtggtgaggt tgccgcaatg
     2221 acgcatctgg aactggttcc cgtcccgcct gtcgcgcaac tggcgggcgt gagccagcat
     2281 tatggaaaaa ccgttgcgct gaacaatatt actctcgata ttccggcccg ctgtatggtc
     2341 gggctgattg gtccggatgg cgtcgggaag tcgagcttgt tgtcgttgat ttccggtgcc
     2401 cgcgtcattg aacagggcaa tgtgatggtg ctgggcggcg atatgcgcga cccgaagcat
     2461 cgccgcgacg tctgcccgcg catcgcctgg atgccgcagg ggctgggcaa aaatctctac
     2521 cacaccttat cggtgtatga aaatgtcgat tttttcgctc gcttgttcgg tcacgataaa
     2581 gcggagcggg aagtccgaat taatgagctg ctgaccagca ccgggttagc accgtttcgc
     2641 gatcgtccgg caggtaaact ctccggcggg atgaagcaaa aactggggct gtgctgcgcg
     2701 ttaatccacg acccggaact gttgatcctt gatgagccaa caacgggggt tgacccgctc
     2761 tcccgcgccc agttctggga tctgatcgac agtattcgcc agcggcagag caatatgagc
     2821 gtgctggtcg ccaccgccta tatggaagag gccgaacgct tcgactggct ggtagcgatg
     2881 aatgccggag aagtgctggc aactggcagc gccgaagagc tgcggcagca aacgcaaagc
     2941 gccacgctgg aagaggcgtt tataaatctg ttaccgcaag cgcaacgcca ggcgcatcag
     3001 gcggtagtga tcccaccgta tcaacctgaa aatgcagaga ttgccatcga agcgcgcgat
     3061 ctgaccatgc gttttggttc cttcgttgcc gttgatcacg tcaatttccg cattccacgc
     3121 ggggagattt ttggttttct tggatcgaac ggctgcggta aatccaccac catgaaaatg
     3181 ctcaccggac tgctgcccgc cagcgaaggt gaggcgtggc tgtttgggca accggttgat
     3241 ccaaaagata tcgatacccg ccgtcgggtg ggctatatgt cgcaggcgtt ttcgctctat
     3301 aacgaactca ccgtgcggca aaaccttgag ttacatgccc gtttgtttca catcccggaa
     3361 gcggaaattc ccgcgcgagt ggctgaaatg agcgagcgtt ttaagctcaa cgacgttgaa
     3421 gatgttctgc cggagtcatt gccgctcggc attcgccagc ggctttcgct ggcggtggcg
     3481 gtgattcatc gcccggagat gttaatcctc gatgaaccta cttctggcgt cgatccggtg
     3541 gcgagggata tgttctggca gttgatggtc gatctctcgc gccaggacaa agtaaccatt
     3601 tttatctcca cccactttat gaacgaagcg gaacgttgcg accgcatctc actgatgcac
     3661 gccggaaaag tgctcgccag cggtacaccg caggaactgg ttgagaaacg cggagccgcc
     3721 agtctggaag aggcatttat cgcctatttg caggaagcgg caggacagag caacgaagcc
     3781 gaagcgccgc ccgtgataca cgacaccacc cacgcgccgc gtcagggatt tagcctgcgc
     3841 cgtctgttta gctacagccg ccgcgaagca ctggaactgc gacgcgatcc gatacgttcg
     3901 acgctggcgc tgatgggaac ggtgatcctg atgctgataa tgggttacgg catcagtatg
     3961 gatgtggaaa acctgcgctt tgcggtgctc gaccgcgacc agaccgtcag tagccaggcg
     4021 tggacgctca atctctccgg ttcccgttac tttatcgaac agccgccgct caccagttat
     4081 gacgagcttg atcgtcggat gcgtgcgggc gatataacgg tggcaattga gatccctccc
     4141 aatttcgggc gcgatatcgc gcgtggtacg cctgtggaac tcggcgtctg gatcgacgga
     4201 gcgatgccga gccgtgccga aacggtaaaa ggttacgtgc aggcaatgca ccagagctgg
     4261 ctacaggatg tggcgagccg acaatccaca cccgccagcc aaagcgggct gatgaatatt
     4321 gagacgcgct atcgctataa cccggacgta aaaagcctgc cagcgattgt tccggcggtg
     4381 atcccgcttc tgctgatgat gatcccgtca atgctaagcg cccttagcgt ggtgcgggaa
     4441 aaagagcttg ggtcgattat caacctttac gtgaccccca ccacgcgtag tgaatttttg
     4501 cttggtaaac agttgccata catcgcgctg gggatgctga actttttcct gctctgcggc
     4561 ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt tcctgacgct caccctggcg
     4621 gcgctgctgt atatcatcat tgccaccgga atggggctgc tgatctccac ctttatgaaa
     4681 agccagattg ccgccatttt cggaacggcg attatcacgt tgatcccggc gacacagttt
     4741 tccgggatga tcgatccggt agcttcgctg gaagggcctg gacgttggat cggcgaggtt
     4801 tacccgacca gtcattttct gactatcgcc cgcggaacgt tctcgaaagc gctggatctg
     4861 actgatttgt ggcaactttt tatcccgttg ctgatagcca tcccgctggt gatgggctta
     4921 agtatcctgc tgctgaaaaa acaggaggga tgatgcgcca tttacgcaat atttttaatc
     4981 tgggtatcaa agagttgcgc agtctgctcg gtgataaagc gatgctgacg ctgattgtct
     5041 tctcgtttac ggtgtcggtg tattcgtcag cgaccgttac gccaggatcg ttgaacctcg
     5101 cgccgatcgc cattgccgat atggatcaat cgcagttatc gaaccggatc gttaacagct
     5161 tctatcgccc gtggtttttg ccaccggaga tgatcaccgc cgatgagatg gatgccggac
     5221 tggacgccgg gcgttatacc tttgcgataa atattccgcc taattttcag cgtgatgtcc
     5281 tcgccggacg ccagccggat attcaggtga acgtcgatgc cacgcgcatg agccaggcat
     5341 ttaccggcaa tgggtatatc cagaatatta tcaacggtga agtgaacagc tttgtcgcgc
     5401 gctaccgtga taacagcgaa ccgttggtat cgctggaaac ccggatgcgc tttaacccga
     5461 accttgatcc cgcgtggttt ggcggggtga tggcaatcat caacaacatt accatgctgg
     5521 cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga acacggcacg gtggaacact
     5581 tgttggtgat gccgataacg ccgtttgaga tcatgatggc aaaggtctgg tcgatggggc
     5641 tggtggtgct ggtggtgtcg ggattatcac tggtgctgat ggtgaaaggc gtgctgggtg
     5701 taccgattga aggctcgatc ccgctgttta tgctgggcgt ggcgctcagt ctgtttgcca
     5761 ccacgtcaat cggcattttt atggggacga tagcgcgttc aatgccgcaa ctggggctgc
     5821 tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg tggatccacg ccgcgcgaaa
     5881 gtatgccgca gatggtgcag gacattatgc tgaccatgcc gacgacacac tttgttagcc
     5941 tcgcgcaggc cattctctac cggggtgccg gattcgaaat cgtctggccg cagttcctga
     6001 cgctgatggc aattggcggc gcgtttttca ccatcgcgct gctgcgattc aggaagacga
     6061 ttgggacaat ggcgtaagga agggaaactt gacttcatac tgtgatgtca taaagtgaag
     6121 tcataaggga atgtcatgta aacacaggta caggctttac gaaagaaaca aaaaaacaca
     6181 ctggatcagc tatttaaatc acctttacct caaggaatca aatggactga tatagaatct
     6241 ctggttaaag cgttaggcgg agaaattaag gaaggtaggg ggtcgcgctg taagttcata
     6301 ctaaatatga gcgttgcgtg tttccatcgg cctcatccgt cgccagatac cgataaaggc
     6361 gctgtagaaa gcgtgcgtga ctggttacta agtatagggg taaaaccatg atcaaactga
     6421 agacgccaaa ctctatggag attgccgggc aacctgctgt tatcacttac gtgccagaac
     6481 tgaatgcatt tcgtggcaaa ttcctgggct tgtcaggtta ttgtgatttt gtctctgaca
     6541 gcattcaggg gctgcaaaaa gaaggggaac tttccctgcg agagtatctt gaggattgta
     6601 aagctgcggg tattgagccg tatgcgcgta cagaaaagat taagaccttt acgttacgct
     6661 atccagaatc gctgagtgaa cgccttaata atgcggctgc gcagcaacaa gtctctgtta
     6721 atacctatat tattgaaaca ctgaatgagc gtttgaatca tttataaatt ttagcggcgt
     6781 acattacgca gaatatctgc ctttacagga tctatttatg caactgaact taattttata
     6841 aattaagttc agttaatatc ttttctttcc tcgtttcaga aataactcaa tcttccttcg
     6901 gcaagcactg caaatgcccg tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat
     6961 gctgcacgtc gcccatatca cctttcagca cggcgatttc cagacagtcg tcgtgattga
     7021 tatgcacatg cagcgtggcg acggagagat cgtggtgatg gtgctgggtg gagacaatgc
     7081 ggctggctaa gtcgcgtttt tcgtgttcat acacatagga gagcaccgcg aaaccttgcg
     7141 tgccgtgctg ctgagtggcc tcctgggcca gagcgctacg cagaatatcg cggatagctt
     7201 cggaacgatt gttataacca cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt
     7261 catcaagcgt gatggtgact cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg
     7321 cacggggaat gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt
     7381 taatttctct cccaccacct gggtttcgac gatttgtccg ttgtccatca ccatcacccg
     7441 ctgacagaag cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa
     7501 ctgttgttgt agctttttca gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt
     7561 agaaactgct tcatccagaa tcagtagttt cggttcgacc gccagcgcgc gagccaggca
     7621 gacgcgctgg agctggccgc cgcttaactg cggtgggcgc ttgtcgagca cgctgtcgtc
     7681 gagatcgacc gccttcagca tttcgctggc gcgcgccagt tgttcggatt ttttcagtga
     7741 tagcaggtgg cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc
     7801 gctgatggag tcctgaaata ccatctgaat atcgcggcgg aacgctttac gctgggcgcg
     7861 attgagtttc gccagcggtt cgccgcgcca gctaatattc ccttgtgagg gagactctaa
     7921 gcctaccagc agccgcgcga gggtgctttt tccgcagccg ctgcgtccca gcagggcgac
     7981 ggtttcgccg cttttcaggg tcagggaaac gttattcagc accgcctgat gttgatgttt
     8041 tccgctaaat ccaccgtggg catagtgatg ggaaaggccg cagacgttaa gtaaggtcat
     8101 gatgccagct ccataccgta gagggcgaga tgagcggaaa ccaggctgcg cgtcaccgta
     8161 tgtttggggg cgttaaacag cgtttctaca tcgccctgtt cgacaatttt accttgtgac
     8221 atcaccgcca cgtcatccgc cagacgcgcc acaacgccca tatcatgggt gaccagcagc
     8281 attcccggcg cttgtttttg cataatgctt tccagcagat cgaggatgcg cgcctgtgct
     8341 accacgtcga ggtcggtggt cggttcatcg gcgatgataa acggtgattc acacagcacc
     8401 gccatcgcaa tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc
     8461 agcacgcgcg cggcgttttc cagccccacc gcttctatgg cagcggtaag cgtagcgtca
     8521 tcggcgggtt tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgcagt
     8581 ggattaaagg cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg
     8641 caaggcgaaa ccggtttgcc atcggctaaa atttcccccg ccgtctggcg aacgccagcg
     8701 ggcaaaatgc ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct accgccgact
     8761 aacgccagca cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg
     8821 gcctgtagcg cgatattacg tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca
     8881 ccagatgagg atccagatga tcgcgcagtg cgtcacccac caggttaaag gccatcacgc
     8941 tgataaacag cgccagcccc ggccagaaca tttgcagcgg ctgggtccag atatactggc
     9001 gcgcgtcgtt aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg aggccaagga
     9061 aagacatccc cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc agcaccagca
     9121 gcgaagggat caccgcgcct gccagatgat cgacaaacac ccgcacatgg cccgcaccgg
     9181 aaagccgtga cgccagcaca aactcgcgtt ggcgtagtga aatcaccagg ctgcgcacca
     9241 tgcgtgcata ccacgcccag tgcgacaggg cgatggcgat aattacgttg gtcagcccgg
     9301 tgccgagcac gccaaccata aagaacgaca gaatcgaggt cgggaaggtc ataaacatat
     9361 cggcgacgcg catggtggcc tgatcaacgc gtccgccaat cagtccggcg ctgccgccaa
     9421 tcaccagccc taatgtcagc accagcagca ggcaggccat taccgaaccg agcgacacgc
     9481 gggtcgccgc catcagccgc gagaaaatat cacgccctaa gtgatcggtg cccagccagt
     9541 gctgcgcatc cggcgaaagc aggcgcgacg gcagatcaat agcctgaggg tcatacggca
     9601 gccaccactg gctggtaagc gcaatcagcg ccagcagggc gataatgatc agcgccaggc
     9661 gtaccgacca gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat
     9721 gcgcgggtcc agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca caaaaaccac
     9781 caccatcatc agcgtaaagc actggatcac cggatagtca cggttaaaaa tcgccgacac
     9841 cgcatagcgc ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg tcccgccgat
     9901 cagttcgccg atgtgcatcc ccacggcggt gatcatcggc agcgaggcat tgcgcaggat
     9961 gtggcgacgt tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccagg tgacgtgacg
    10021 ctgaccggcg acgtccagca tactggcgcg cagtaaacgc gcgttaatcg ccagcgacat
    10081 aaaggcaatg gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg
    10141 tagccattgc agatacaccg aaaacgccat caccagtaaa aacgccagcc agaagttagg
    10201 catcgacacg ccaagaaacg cgatgaaacg tacggcgaaa tccggcagac ggtcgcggtg
    10261 gcgcgccgcc cagataccga gcggtacgga agtgagcaga attaatacca gcgccgcacc
    10321 tgcaagttcc agcgtggcgg gcaggaagtt cagcatatcg tccagtaccg ggcgttggct
    10381 ggcgaatgag ataccaaagt caagatgcag cgccttccac aaccaggtgc cgtactggac
    10441 gtacagcggc tgatccagtc ccagcatggt gcgggtagag gccagcatct ccggcgtcgg
    10501 cggcaggtta gacagacgca aataatcgag cgccgggtcg ccggtaccga ggcgcagcat
    10561 cagaaaaatg atcaccgagg cggcgagcac catcgggatc agcagcagaa agcggcgtaa
    10621 tacgtaacgc aacattaagg tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg
    10681 atcggcgcgt aggggatgtt acccagctcc ggttttgata ccaccatcat cgagatgtag
    10741 ctgataggca gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg atacagcgcc
    10801 tgacgttgcg tttcgtcatg ggtcgccagc acttcgccga tctctttatc aatcagcggc
    10861 ttgtcggcta atccttgctg tgcctggaag tcagcgtgcg acggtacgcg cattgaactc
    10921 aggaaggcgt gtggatcgta tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga
    10981 ccgtcgcgct gacgagcata gatactgctc tcttcttcgc caatcagcga gacatctgcg
    11041 ccaatctggc gcatatcagc ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg
    11101 ccgatgaacg acagttcaat gcgcagcggc tgaccatttt tctcgcggat gtctttgccc
    11161 gcaggcagcg tccaaccggc tttttccagc aactctttcg ctttctgcgg atcgtactgg
    11221 cgcggtttca ggccgagatt agcgtaaggc acagaagggg caaacagggt gtcggcgacc
    11281 tgctgggtgc catataacgc gttatcaatc agcgattttt tgtttaccgc gtaattaaga
    11341 gcttcacgta ctgccagctc gttggtgggg gctttggcgg tattgagcgc cagcatcacg
    11401 gtttcgatcg gctgtgacag ttgggtgtgg taagccggat tctggctaaa gcgggcgaag
    11461 gtatcgagcg gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac
    11521 gccaccgcgc gggtagtcgg gtccgggatg acgttaaagg tgatcttttt aatcgctggc
    11581 ttttcgcccc agtagttttc gttacggacg aagacatcgt actgattcag tttcgattcc
    11641 tgcaaaatcc acggtccggt gccaatcggc gctttaattc cgttcatggt ttcatggttt
    11701 ttaaactgcg agggagcgat aaaacggaaa ggacggggca gggccagttc ttgcaggaaa
    11761 ggatagtagg cgcttttcag ggtaatttgc agctctgttt tactgagtgc tttaacatca
    11821 acaatctggt ttgccagctc cagccaggcg tgacgttgac ggttatcgag cactgcgcgg
    11881 aagttttctg ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt cacgtcatca
    11941 cgcagggtga aggtccaggt tttaccatct tctgaatgag tccagctttt tgccagccac
    12001 gggatcaccg aaccgtctgc ctgatatttc accaatggtt cataaaccat gctctgggcg
    12061 aacatctggt taggcgtgta aaggtgcggg tttagtggcc cgacattcac cggccaggcg
    12121 gtggtgattt catctggtgc agcggcatgg acgataaaag acgcacaagc cagcagcgca
    12181 aatagagtgc ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa tagatggcgg
    12241 aaataagtat gacgatttta agtattcgtc atactgatta cctgttctgg atcaatagat
    12301 gggcagtcgg gtgggcggat cagttaactg aatcgatcca ttgcacactg tcggcggtga
    12361 gcgtaaaggg agtaggggtg cagatcgcca ggcttaaatt ttcaagctga caatggctga
    12421 ctgacagcga ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg ctgccgccac
    12481 gctgtttaac gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat
    12541 caggatgcac ggcgtccatc tcagcgtgtt ccccgaggct gaatacggcg ttcgccagcc
    12601 agcgccagtt ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg ccttcatcac
    12661 tcaacagcag ggcgatatcg tcaccgctat ggcttaagtt gaaccatagc ggcgtttccg
    12721 gcgcaaatgc aggtttgcct tgttcgccat agatgatctc cggtagcggg gaaagcgtgt
    12781 gcgaaagcaa tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct
    12841 cgcgtaaacc cggtggcagt ggagctgcgc ttaaggtcga aactttcccc agaactatcc
    12901 gatacatatc agggccaacg tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc
    12961 ctcatcccgg taaccgactt ttcggtctgc ctggccccag taaaatcgcc agtttgctac
    13021 cgcctttggt ggtttccatc cagattttac acacactggt taatggcacc gaaagtagca
    13081 tccctaccgg gccgagcagc catccccaaa ttaacaatga aagaaatacc accatggtgg
    13141 acatccccag gcgatggccc atcatccgtg gctctaaaat attgccgatg accatatgga
    13201 caactaaaaa caatgcgccg accagaatac attcgtaaat accattaaac agcagcacct
    13261 gaatcattgg cggtacggcg gaaattaccg cgccgatatt gggcacgtag ttaagcaaaa
    13321 acgccagtac tgcccacatc agcgcaaact gcacccccat cagctccagc cccagccaga
    13381 cgatgacacc tgtccataaa ctgagtagcg tctttaacgc cagataatgc gaaacgcctt
    13441 taagtgcgcg gtgtaatcct gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt
    13501 tgtaagggac gtggcgcact tcaaacagca taaaaactac ggtcatcacc agcaaaagca
    13561 cgctcgccat tgccccggaa agcccggtca ttagcgctgt ggtgaaggta accacttttt
    13621 ccgagtccat ccgctgcagc attcgctccg gcgacatatg caaattaaga aaaggcaaca
    13681 tctcctgcaa tttaaaaagt ttgcgcgtca gctccttatt aaacttcggc agcatagaga
    13741 taaattcgtt aaacgatgcc gccagtacgc cgaccagcgc ggttagtgtg atcagcatca
    13801 ccaccaccac aatcgtaatg gcaacggggc gttttactcc ccgtcgaata aaccaggtaa
    13861 cgagcgggtt gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg
    13921 ccgctgcgtg aatgcccgcg aggatcacta ccagcgaggc cagcttgagc agaatgtgca
    13981 tgcccgtttt atcgggttga ggggtttcca ttggggcttc cttgtgactt tttgtattaa
    14041 gtgtagcggg agtcacgcca gcaatattct gattcttact gctgattttc acgtcagcac
    14101 atggtaaaaa tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc
    14161 gaacccgcgc taaacggatt gcgcctgaat ttgcgcattg tctccattgt catgtttaac
    14221 ttcgccagct acctcaccat cgggttgccg ctcgctgtat taccgggcta tgtccatgat
    14281 gtgatgggct ttagcgcttt ttgggcagga ttggttatca gcctgcaata cttcgccacc
    14341 ttgctgagcc gccctcatgc cggacgttac gccgatttgc tgggacccaa aaagattgtc
    14401 gtcttcggtt tatgcggctg ctttttgagc ggtctgggat atctgacggc aggattaacc
    14461 gccagtctgc ccgtcatcag cctgttatta ctttgcctgg gacgcgtgat ccttgggatt
    14521 gggcaaagtt ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg
    14581 catatcgggc gggtgatttc gtggaacggc attgtcactt atggggcgat ggcgatgggt
    14641 gcgccgttag gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc gttaatcatt
    14701 atgggcgtgg cgctggtggc cattttgttg gcgctcccgc gtccgacggt aaaagccagt
    14761 aaaggcaaac cgctgccgtt tcgcgcggtg cttgggcgcg tctggctgta cggtatggcg
    14821 ctggcactgg cttccgccgg atttggcgtc atcgccacct ttatcacgct gttttatgac
    14881 gctaaaggtt gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt
    14941 acgcgtttgt tattccctaa cggcattaac cgtatcggcg gcttaaacgt agcgatgatt
    15001 tgctttagcg ttgagataat cggcctgcta ctggttggcg tggcgactat gccgtggatg
    15061 gcgaaaatcg gcgtcttact ggcgggggcc gggttttcgc tggtgttccc ggcattgggt
    15121 gtagtggcgg taaaagcggt tccgcagcaa aatcaggggg cggcgctggc aacttacacc
    15181 gtatttatgg atttatcgct tggcgtgacc ggaccactgg ctgggctggt gatgagctgg
    15241 gcgggcgtac cggtgattta tctggcggcg gcgggactgg tcgcaatcgc gttattactg
    15301 acgtggcgat taaaaaaacg gcctccggaa cacgtccctg aggccgcctc atcatcttaa
    15361 aacttactga ataaccagcg tattaatgat gttttctgcg gttgtctgcg ctttttgctg
    15421 atcgtcagct ggcagcgtaa tttgcatggt tagcagttga ttacccacgt tacccagaat
    15481 aacagaagag tacgccgtct ggcctttcgc ggagataata ctgtctaact gctgcatttt
    15541 gtgacctttc agctcaatgg ctttattggt taccacttgc agctgcggat cgcggctacg
    15601 ttgctgatct tccagacgct tcgccagcac cgccagatct tctttcggat catcgcccat
    15661 gatgacgatg actgctttct gaccggtggc gtcggaccag acatgcatgt tattggcctg
    15721 cgttcccagc ttaccgctct ggtcggtcat atccgctggc agcgagaaac ttaacttgcc
    15781 atcaagcagg ttgacgggat tcccggtagc gttactttcc gcgaccgaac cctgcgccgt
    15841 agcgttagtg tctttatcat cacaggccgc aagccccata accagcaggc caattccgac
    15901 atatttaacc agattgcgca ttgacttctt cctttcgata aacggccata acggctcatt
    15961 catccatctt atcacaactc tgataacgaa cctttaactc gcctgcaaag cgttgatttc
    16021 ggatttatct gccagtcttt tcaacagcat attgagtaat acgccataca ttggcaggaa
    16081 gaaaacgata ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg
    16141 ttcagccata aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc
    16201 gctgacgtta ccgaacagtg tggacgctgt cggtgccagc caccagcggc gactctgacg
    16261 caggcggtta aaaacgtgca cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc
    16321 ggtggcgata cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc cctgccatga
    16381 acccatatag aatagcgacg agatgacgta ggagatcagc aatgccggga tcatcaccgc
    16441 aaagatgata cgacgggcca gcggtgcgcc aaaaatacgt acggtcaggt cggtagcgag
    16501 aaagataaac ggaaagctaa acgcgcccca ggtggtatgg aaaccaaaaa tggagacggg
    16561 aagctgaacc agatagttac tggaggtgat caccagcaga tgaaataacg ataaccagaa
    16621 caacgcttta tagcgttgag tttgcgagaa aacgttcata ttgtaccttt ttgattaacc
    16681 attggggtga gggaacccaa tacgtacgac acgtctgtta ccttaacata ataacgtgct
    16741 tacccttttt tcgagccgcc gcatgatact gctttacgtt gacaatgcaa tggttaattt
    16801 tcacgcaatc gttaacctgg tttgcttacg gactcacagg gcgtaaacta gcgccgtttt
    16861 tttatgtgat gagaagaaaa tgaccgatct cttttccagc cctgaccaca cactcgacgc
    16921 acttggcctg cgctgcccgg aacccgtaat gatggtgcgc aaaacagtgc gcaatatgca
    16981 gccaggtgaa acgttgctga ttattgctga cgatccggcg acgacccgcg atatccctgg
    17041 gttttgtacc tttatggaac acgaactggt tgccaaagag acggatggac tgccttatcg
    17101 ttatttgatt cgtaaaggcg gttgatgggg aaagattggc ttcgatgccg ccttttcccc
    17161 tcaccctaac cctctcccca gaggagcgag gggaccgatc gcgctcaatg ttgcgatcgg
    17221 tttgccttat ctcctgcgca acaatcttaa cgcattcgct gtcaccagca ccgtagcacc
    17281 cgtatctgcc agcaccgcca gccatagtcc ggtcattcct aacagcgtgg tgacgaggaa
    17341 gatccctttc agccccagcg caatagtgat gttctggcgg atattggcgt gagtggcgcg
    17401 tgccagttca atcatttgca ccagaccgcg caggtggtta tgggttaatg ctgcgtcggc
    17461 ggtttccagc gccacgtctg tgccgctgcc cattgcaatc ccgatggcgg cagctttcat
    17521 cgctggcgcg tcgttaatac cgtcaccgac catcgccagc ggcgcatgtt gattcagctc
    17581 ggtcaccgct ttgactttat cttccggcaa caggcccgct ttaaactcca gccccagctc
    17641 cccggcaatt gccgccgctg cgcgtggatt atcgccggtg aggatcaccc ctttgacgcc
    17701 cagcgcgttc agttcactga tggcagttgc agcatcggcg cgcagggtat cctgtaacgc
    17761 aatgacaccc agcacgtcat cgttacgtac taccagcact accgtttgcc cggcgctttc
    17821 cagttcgtta attaaaccag caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa
    17881 tacgcgctca ccgttaacct gcgcttcaat gccagacccg accagcgccc gctgtgattc
    17941 ggcggcggga atggcgagtg cagcaacctg tgcttcgcgc acgatggcct gcgccagtgg
    18001 atgcgtcgcg ccttgctcga ccgccgccgc cagtgtcagc agttcagatt cactaatacc
    18061 cgttgccgga tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac cggttttatc
    18121 aaacgccacc tgggtaacac gacctagctg ctccagcgcc gcaccgcctt taattaacgc
    18181 cccacgacgt gccgctgctg ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa
    18241 cgcacacggg cagccaatca gcagcagcgt caggccttta taaatccact cttgccagct
    18301 ggcggcaaac agcagcggcg gcaccagcgt taccagcagg gcgacggcca taattgctgg
    18361 cgtataaatg cggctgaaac ggtcgataaa ccgctcaatg ggagcgcgac gctcttcggc
    18421 ttcttcgatc agtttcagaa tccggtcaat ggcgctggct cccggttcgg acaatacttc
    18481 caacgtcacc agacggtcta cgctggtggc gcctgcagga actttatcgc ccgtcgcacg
    18541 ctccaccgga atggattcgc cggtcagggc gctttcatca aaactggcaa agggtgagag
    18601 cagtttaccg tcggcaggca aacgcccacc cgccgcaact tcaatcacat cgccagggcg
    18661 caggctgtta attgccacct cttcacgctc accgttacgc aggcgcgtgg cgttttctgg
    18721 tttcagcgcc attaacgcgc tgacgccctg acgcgcgcgg ctggcggccc aaccttccag
    18781 tcgttcacca atcaaaaaca gcagcaacac catcgcagct tcagccgttg cgccaataaa
    18841 cagcgcacca atagcggcta cgctcattaa cgtttcaatg gcgaagtaac tgcctgattt
    18901 gatcagccgt aatgcctgac gagcaatcgg gtacagccca accagcgtgg tcgcgataaa
    18961 cgccagttgc ccgaacggat gattgaactg ctccagaccc cagctgattg ccatcatcac
    19021 gattagcgta atcagcgaca gattctcttt caggcgtgat gcttgcggtt cttcggcggc
    19081 ctgttcatcg cgcagggaat agcctgcttt ttgcaccgca gattcaacct gggcacggat
    19141 gtcattgtcg gcatcaacca ccagtttttc ggtggcgaac aacacctgta cctgattcac
    19201 gcctgcaagc tggcgcacgg cattttctac cttgcgcgca caggcggcgc agtccatgcc
    19261 gctgactttc cagctatagc gggtgccgga gacgttttca gagagagttg gcgtgctgga
    19321 acatgcgccg tcgcagcaac agtcgttggc gttctgtacc gtggttagcg gtttgaacgc
    19381 agcaaattga ggggctttct tgccgtgatt gtcaggagtc gacatggcat cctccggtta
    19441 agttttttct cattaaccga aggatacact ctggagtcga ctccagagtc aagttttatc
    19501 agagatacag cgagcggacg atcaggaagt gaccggcaaa gtagcaggcc gcagcaatcg
    19561 cgttatccgc acggaagcgt cggcgatagt gactacccag ccagacaaag ttactgataa
    19621 acagcaacga agcgccgaca aacgcagaga gcgccggagc ggtcggacgg aagaaccaca
    19681 gttcacctgc cagccacacc atcaccagcg tcatgccgat aaacgtgcag ataggccaac
    19741 gatactcttc cagccgcgtc cagataatcg ccagtaacag cgcgcccaga accagcagca
    19801 ccagtggcag aggccagaag aaagagagcg tcatctgact ggcgaaatag atggtgtaca
    19861 gcaggtgcga gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg
    19921 ttagcgcatc gcccagcagt gaggcgcaca gccccgccag caccagatag ctaatggcgt
    19981 cgaacatcgg cgcttgccag gccagtaaca gcaggagaag aagggttaat ggtttaaata
    20041 cccagcgttg ccaggttggc ccacgatacg atgcatccac agatagccat gcggaaagac
    20101 agacagcgat aaacgaccaa agcatcttag ttccttgaat tcgttatttt ttcgtaagcg
    20161 gaatgcctac ggtcttgctt ccagtttagt ggcgagggtg gacggatgac aacaccataa
    20221 ctggcagggt atgcttaatt ccgcattttc cgatgaggga taaagatgag caaaccacct
    20281 cttttcttta ttgttatcat tggcttaatc atcgtcgccg catcgtttcg ttttatgcaa
    20341 caacgacggg aaaaagctga taatgatatg gcaccactcc agcaaaagct ggtgttggtg
    20401 agcaacaagc gggaaaaacc gattaacgat cgccgttcgc gccagcagga agtgactccg
    20461 gcaggcacca gtatgcgcta tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag
    20521 acgtttcgcc tcgacgccca gcagtaccac gccctgacag tgggcgataa aggtacgctg
    20581 agctataaag gaacgcgctt tgtcagcttt gtaggcgaac aataaggtta ttttttaact
    20641 ttaaatttct tctgccagac cagcagttca aacacgccga aaaggaaaat ccgtaccttt
    20701 tcggcggtgg tcatctgcgg gccatctttc ggtaaagtgg atttcaatag cgccagctgc
    20761 ataccatgca tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt cagcggacgt
    20821 gggaagggat gcaccaggtt gaggattaaa aatccccaaa cacagagcat taacaaacga
    20881 ccaatattaa tcagcatcac tttctccttg tgcttcgcgt tgatacagcc gataagccac
    20941 ctgacccgcc actttttccc gatgtaatga ccagtttgct ggaacagtgg gcagaccgtt
    21001 ttcgacttcg ctttcgacat aaatcagggc ttcgtcagcc agccagccgt tatcttccag
    21061 taaatttatc gtctcttcta acaagccacg gcggaacggt ggatcgacaa acacgatatt
    21121 atgcggtgtg cctttttgcg ccaggaatga cattgcgttg ctgttcacca cgcgtgcatt
    21181 gcctgctttt agtgtcgcca gattcttaat taactgctga gaaaccgcgc gatccatctc
    21241 aatcaacgtg gcccccgcag catagcgcga caacgcttcc agccccagcg cgccgctccc
    21301 ggcgaagcaa tccagacatt gggcgtcaac aatgaccgga gccagccagt taaacaacgt
    21361 ttcgcgtacg cggtcggtgg tggggcgcag acctgggcta tcaggaaccg ggagtttacg
    21421 gcctcgccac tgcccgccaa taatgcggat ttggccgctg ccggaatgat tcggtttttt
    21481 catgataaat tgctcaatcc gccagataac acatacttgc aggcggtgat gtgaattaag
    21541 ttaagtgata gactatttca tcattttttt agctgctatg tacatagcgt taacgctgtg
    21601 ccatgaagca acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt
    21661 tttcctggct gggctttggt caaaaagagc agaccccgga aaaagagaca gaagttcaga
    21721 atgaacaaac ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag gcttctgaac
    21781 aagccgtcga agagcagccg caggcgcata ctgaggccga ggcggaaact tttgctgccg
    21841 acgttgtgga agtcactgaa caggttgctg aaagtgaaaa agcgcagcct gaagcggaag
    21901 tcgttgcaca accggaaccg gtcgtagaag aaacgccgga gccagtggct atcgaacgtg
    21961 aagagctgcc gttgccggaa gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg
    22021 ctgaagcgga aaccgtagag attgtcgaag cggcggaaga agaagcggct aaagaagaaa
    22081 tcaccgacga agagctggaa gcacaggcgc tggctgccga agcggcagaa gaagcggtga
    22141 tagtggttcc tccggcagaa gaagagcagc cggttgaaga aattgcccag gagcaggaaa
    22201 aaccgaccaa agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa
    22261 atctcggttc cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg
    22321 aggagctgga agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaataa
    22381 tcaccaatct gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg
    22441 gcctgctgaa agaagagatg ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg
    22501 aaggtaaaac gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaaccacga
    22561 cgattggtaa gctggcgcgt cagtttgagc agcagggtaa atcggtgatg ctggcggcgg
    22621 gcgatacttt ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata
    22681 ttccggtgat tgcccagcat accggtgcgg attccgcctc tgttatcttc gacgccattc
    22741 aggcagctaa agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga
    22801 acaaatcgca cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg
    22861 ttgaagcgcc gcatgaagtt atgctgacta ttgatgccag taccgggcag aatgcggtaa
    22921 gccaggccaa actgttccac gaagctgttg gcttaaccgg catcacgcta acgaaactgg
    22981 acggcacggc gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc
    23041 gctacattgg tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta
    23101 tagaggcact ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc
    23161 ttatctcggt gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat
    23221 ggcgtttctg accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg
    23281 gattgagcgg cccagcgccg ggaaaatctg gtttagcggc catgacatca cgcgtctgaa
    23341 aaaccgtgaa gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct
    23401 actgatggac cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag
    23461 cggtgacgat attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa
    23521 agcgaagaac ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg
    23581 cgcggtggtg aataagcccg cggtactgct ggcggacgaa ccgaccggta acctggacga
    23641 cgcgttgtcg gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt
    23701 attgatggca acgcacgaca tcaacctgat ttcacggcgt tcctatcgca tgctcaccct
    23761 gagcgatggt cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca atcaatcata
    23821 ttcggcagtt tggcgggcgt cttgatcgct tccgtaaatc ggtcggcggc tctggcgacg
    23881 gcggtcgtaa cgccccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgtaaaacca
    23941 acgttttcaa cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca
    24001 aaccgttcgc cacgttttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg
    24061 tctgttatat ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc
    24121 aaatcactgt ttacttgcaa aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac
    24181 agttgcaggc cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg
    24241 gcgagttccg taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc
    24301 ttccggcagt ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata
    24361 cgctgcgtga tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct
    24421 ggtttgcccg tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg
    24481 tgttgatggt ggcggcagtg ttcctcgtca tcggcaacag cgtacgtctg agcatctttg
    24541 ctcgccgtga ctccatcaac gtgcagaaac tgattggtgc gacggatggt ttcatccttc
    24601 gcccgttcct gtatggtggc gcactgctgg gattttctgg cgcattgttg tcattaattt
    24661 tgtcagaaat tctggtgctg cgattgtcat cggcggttgc ggaagtggca caggttttcg
    24721 gaacgaagtt tgatatcaat ggtttatcgt tcgatgaatg cctgctattg ctgctggtat
    24781 gctcgatgat tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta
    24841 cgcctgaata ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa
    24901 gagatcccgt tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat
    24961 tcacaagctt gcattgaact tgtggataaa atcacggtct gataaaacag tgaatgataa
    25021 cctcgttgct cttaagctct ggcacagttg ttgctaccac tgaagcgcca gaagatatcg
    25081 attgagagga tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac
    25141 ctggattcct acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg
    25201 gcgctggctg aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg
    25261 tctcacctgc ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag
    25321 gcggatttga ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg
    25381 gaagtgggtg tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa
    25441 tacgttctgc gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg
    25501 ttcttcaacc tgcgtaaaac caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa
    25561 atggtggccc gtgaactggg cgtaaccagc aaagacgttc gtgagatgga atcacgtatg
    25621 gcggcacagg acatgacctt tgacctgtct tccgacgacg attccgacag ccagccgatg
    25681 gcaccggtgc tctatctgca ggataaatca tctaactttg ccgacggcat cgaagatgat
    25741 aactgggaag agcaggcggc aaaccgtctg accgacgcga tgcagggtct ggacgaacgc
    25801 agccaggaca tcatccgtgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa
    25861 ctggctgacc gttacggtgt ttccgccgag cgtgtgcgcc agctggaaaa gaacgcgatg
    25921 aaaaaacttc gcgctgccat tgaagcgtaa tttctactac taagcagaga accctggatg
    25981 agaatccggg gtttttgttt tttgggcctc tgcaataatc agtttccccc tccggcaaaa
    26041 cgccaatccc cacgcagatt gttaataaac tgtcaaaata gctattccaa tatcataaaa
    26101 atcgggtatg ttttagcaga gtatgctgct aaagcacggg tagtcatgca taaaacgaaa
    26161 taaagtgctg aacaacaaca tcacaacaca cgtaataacc agaagaatgg ggaacctcag
    26221 gatgaacatg aagggtaaag cgttactggc aggatgtatc gcgctggcat tcagcaatat
    26281 ggctctggca gaagatatta aagtcgcggt cgtgggcgca atgtccggtc cggtggccca
    26341 gtacggtgac caggagttta ctggcgcaga gcaggcggtt gcggatatca acgctaaagg
    26401 cggcattaaa ggcaacaaac tgcaaatcgt gaaatatgac gatgcctgtg acccaaaaca
    26461 ggcggttgcg gtggcgaaca aagtcgttaa cgacggcatt aaatatgtga ttggtcacct
    26521 ctgttcttca tcaacgcagc cagcgtcaga catctatgaa gatgaaggca ttttaatgat
    26581 caccccagcg gcaaccgcac cagagctgac cgctcgtggc tatcagctga tcctgcgcac
    26641 caccggcctg gactccgacc aggggccgac ggcggcgaaa tatattcttg agaaggtgaa
    26701 accgcagcgt attgctatcg tccacgacaa acagcagtac ggcgaaggtc tggcgcgtgc
    26761 ggtccaggac ggcctgaaga aaggcaatgc taacgtggtg ttctttgatg gcatcaccgc
    26821 cggggaaaaa gatttctcta cgctggtggc gcgtctgaaa aaagagaata tcgacttcgt
    26881 ttactacggc ggttatcacc cggaaatggg gcagatcctg cgtcaggcac gcgcggcagg
    26941 gctgaaaact cagtttatgg ggccggaagg tgtggctaac gtttcgctgt ctaacattgc
    27001 gggcgaatca gcggaagggc tgctggtgac caagccgaag aactacgatc aggttccggc
    27061 gaacaaaccc attgttgacg cgatcaaagc gaaaaaacag gacccaagcg gcgcgttcgt
    27121 ctggaccacc tacgccgcgc tgcaatcttt gcaggcgggc ctgaatcagt ctgacgatcc
    27181 ggctgaaatc gccaaatacc tgaaagcgaa ctcggttgat accgtgatgg ggccgctgac
    27241 gtgggatgag aaaggcgatc tgaaaggctt tgagttcggc gtatttgact ggcacgccaa
    27301 cggcacggca accgatgcga agtaacagat agataaatga aaatgcccga tgcgctgaac
    27361 tgccatcggg tttttttgtg tttgtcgaaa tggttgtaga aagattaaac aatccaggat
    27421 atgcttaatg tacggtgaca taccgtacat attatacgga gcaggagagt tcacatgtct
    27481 gctgttaaaa agcagcgtat cgatctgcgt ttaactgacg atgacaaaag tatgattgag
    27541 gaagcggcag cgatatctaa tcagtccgtc agccaattta tgttgaacag cgcttcgcag
    27601 cgggctgcgg aagtgattga acagcatcgg cgggtgatcc tcaatgagga atcctggacg
    27661 cgggtgatgg atgcgctgag taatccaccg tcacctggtg aaaagctaaa acgtgcggca
    27721 aaacgtcttc agggaatgta ataatttatg gatgatctga cgatagagat tctgaccgat
    27781 gatgcagatt atgatctaca gcgattcgac tgcggcgagg aagcgttaaa tctctttctg
    27841 acgacacatc tcgttcgtca acatcgcaac aaaattctgc gagcgtatat cctttgtcgc
    27901 aacactccag aacgtcaggt gctgggatat tacacattat gcggcagttg ttttgaacga
    27961 gccgcattgc cctcgaaatc gaaacagaaa aaaattccct acaaaaatat tcccagcgtt
    28021 actcttgggc gtctggcaat tgatcgttca ttacaggggc agggatgggg agcaacactg
    28081 gttgctcatg ccatgaaagt cgtctggtca gcctctttag cggtaggtat tcacggtctt
    28141 tttgtcgagg cgctgaataa aaaagcccat acgttttatc aatcgctggg ctttatcccg
    28201 ttggtcggag aaaacgaaaa tgcgttattt ttcccaacca aatccatcga actgcttttc
    28261 acacagagcg attaacgctt ctcccagccg ccctgttgcg ccgtaaatcc cagtgcctgc
    28321 ataaacgccg tcatcacacc gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa
    28381 acggcaggat tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacggcgg
    28441 gtgacttccc gcacgcgcag ggaatccagt gctccctcgg tgccgcttaa ggttacccgc
    28501 acggcagcga gcaggcgctc gttaaaacgc gcggcgtaga tacggtggtt atcgtcaacc
    28561 tgtaaagagg aaggggaata ctccggccag attttttgca ggtcaatccg gtcctggtcg
    28621 ctaaagtttt ctaatcgaat gatggtcagc ttcatgggta acccgtgtaa atcacaaaag
    28681 tggaaccagt gtagcgaaat aatttaatcg gaggctttct cttttttatt tcttttggca
    28741 ggtgattaat tttttaacag caataattac aaagttaaaa cattagagaa tgaaaaat
//
LOCUS       DAZFRN010000052        28475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFRN010000052 DAZFRN010000000
VERSION     DAZFRN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.052"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            15..1016
                     /gene="yqiI"
                     /locus_tag="ACHKUB_002187"
     CDS             15..1016
                     /gene="yqiI"
                     /locus_tag="ACHKUB_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6655200.1"
                     /translation="MDCYAEHEGGNTVVIGYVPRISIPSNGKKGDKIWQSSEYFMNVF
                     CNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSYIFGVTYNGVDYDSTAPNAIAA
                     PECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPARVQLYIKLAKNANRVNKNLVLP
                     DEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLDCFVNVALEPADGVVDFGKINS
                     RTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLKIFH
                     KDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITYN"
     gene            complement(1059..1259)
                     /gene="glgS"
                     /locus_tag="ACHKUB_002188"
     CDS             complement(1059..1259)
                     /gene="glgS"
                     /locus_tag="ACHKUB_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO6655201.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            1528..2157
                     /gene="yqiJ"
                     /locus_tag="ACHKUB_002189"
     CDS             1528..2157
                     /gene="yqiJ"
                     /locus_tag="ACHKUB_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6655202.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            2184..3845
                     /gene="yqiK"
                     /locus_tag="ACHKUB_002190"
     CDS             2184..3845
                     /gene="yqiK"
                     /locus_tag="ACHKUB_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO6655203.1"
                     /translation="MDDIVNSVSSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            4084..4143
                     /locus_tag="ACHKUB_002191"
     CDS             4084..4143
                     /locus_tag="ACHKUB_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6655204.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            4460..4519
                     /gene="ibsE"
                     /locus_tag="ACHKUB_002192"
     CDS             4460..4519
                     /gene="ibsE"
                     /locus_tag="ACHKUB_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO6655205.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(4639..6072)
                     /gene="hldE"
                     /locus_tag="ACHKUB_002193"
     CDS             complement(4639..6072)
                     /gene="hldE"
                     /locus_tag="ACHKUB_002193"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO6655206.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(6120..8960)
                     /gene="glnE"
                     /locus_tag="ACHKUB_002194"
     CDS             complement(6120..8960)
                     /gene="glnE"
                     /locus_tag="ACHKUB_002194"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO6655207.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSHSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(8983..10284)
                     /gene="ygiF"
                     /locus_tag="ACHKUB_002195"
     CDS             complement(8983..10284)
                     /gene="ygiF"
                     /locus_tag="ACHKUB_002195"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO6655208.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            10526..11146
                     /gene="ygiM"
                     /locus_tag="ACHKUB_002196"
     CDS             10526..11146
                     /gene="ygiM"
                     /locus_tag="ACHKUB_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO6655209.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            11210..12448
                     /gene="cca"
                     /locus_tag="ACHKUB_002197"
     CDS             11210..12448
                     /gene="cca"
                     /locus_tag="ACHKUB_002197"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO6655210.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAIASWKEQRCPKPD"
     gene            complement(12611..13432)
                     /gene="bacA"
                     /locus_tag="ACHKUB_002198"
     CDS             complement(12611..13432)
                     /gene="bacA"
                     /locus_tag="ACHKUB_002198"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6655211.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(13522..13890)
                     /gene="folB"
                     /locus_tag="ACHKUB_002199"
     CDS             complement(13522..13890)
                     /gene="folB"
                     /locus_tag="ACHKUB_002199"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO6655212.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            13995..14612
                     /gene="plsY"
                     /locus_tag="ACHKUB_002200"
     CDS             13995..14612
                     /gene="plsY"
                     /locus_tag="ACHKUB_002200"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO6655213.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(14625..15557)
                     /gene="ttdR"
                     /locus_tag="ACHKUB_002201"
                     /gene_synonym="dan"
     CDS             complement(14625..15557)
                     /gene="ttdR"
                     /locus_tag="ACHKUB_002201"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO6655214.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            15764..16675
                     /gene="ttdA"
                     /locus_tag="ACHKUB_002202"
     CDS             15764..16675
                     /gene="ttdA"
                     /locus_tag="ACHKUB_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO6655215.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            16672..17277
                     /gene="ttdB"
                     /locus_tag="ACHKUB_002203"
     CDS             16672..17277
                     /gene="ttdB"
                     /locus_tag="ACHKUB_002203"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO6655216.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            17326..18789
                     /gene="ttdT"
                     /locus_tag="ACHKUB_002204"
     CDS             17326..18789
                     /gene="ttdT"
                     /locus_tag="ACHKUB_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO6655217.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(18832..19845)
                     /gene="tsaD"
                     /locus_tag="ACHKUB_002205"
     CDS             complement(18832..19845)
                     /gene="tsaD"
                     /locus_tag="ACHKUB_002205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO6655218.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            20083..20298
                     /gene="rpsU"
                     /locus_tag="ACHKUB_002206"
     CDS             20083..20298
                     /gene="rpsU"
                     /locus_tag="ACHKUB_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO6655219.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            20409..22154
                     /gene="dnaG"
                     /locus_tag="ACHKUB_002207"
     CDS             20409..22154
                     /gene="dnaG"
                     /locus_tag="ACHKUB_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO6655220.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDAVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            22349..24190
                     /gene="rpoD"
                     /locus_tag="ACHKUB_002208"
     CDS             22349..24190
                     /gene="rpoD"
                     /locus_tag="ACHKUB_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO6655221.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(24269..24775)
                     /gene="mug"
                     /locus_tag="ACHKUB_002209"
     CDS             complement(24269..24775)
                     /gene="mug"
                     /locus_tag="ACHKUB_002209"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO6655222.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKRAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            24900..24975
                     /locus_tag="ACHKUB_002210"
     tRNA            24900..24975
                     /locus_tag="ACHKUB_002210"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24933..24935,aa:Ile,seq:cat)
     gene            complement(25029..25793)
                     /gene="nfeF"
                     /locus_tag="ACHKUB_002211"
                     /gene_synonym="yqjH"
     CDS             complement(25029..25793)
                     /gene="nfeF"
                     /locus_tag="ACHKUB_002211"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO6655223.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            26070..26693
                     /gene="nfeR"
                     /locus_tag="ACHKUB_002212"
                     /gene_synonym="yqjI"
     CDS             26070..26693
                     /gene="nfeR"
                     /locus_tag="ACHKUB_002212"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO6655224.1"
                     /translation="MSHHHEGCCKHEGQPRHEDCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKTIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(26847..28367)
                     /gene="aer"
                     /locus_tag="ACHKUB_002213"
     CDS             complement(26847..28367)
                     /gene="aer"
                     /locus_tag="ACHKUB_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO6655225.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASTCFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
BASE COUNT         6948 a         7703 c         7182 g         6642 t
ORIGIN      
        1 tacctgtatg ggcaatggac tgctatgctg aacatgaagg gggaaatacc gtagtcattg
       61 gttacgtacc aagaatttct atccccagta atggtaaaaa aggtgataag atctggcaaa
      121 gcagtgagta ttttatgaat gtcttctgta ataatgcatt acctgcccca tcacctggag
      181 aagaataccc atcagcctgg actaatataa tgatgttttt accaggcggt caggactttt
      241 ataatcagaa ctcctatatt tttggcgtaa catacaatgg agttgattat gattcgactg
      301 cccctaatgc tatcgcagca ccagaatgca ttgatataaa aggagcgggt acctttaata
      361 atcactataa aaaccccgct gtctgtagtg gaggtcctga gcctcaatta tcagtaacct
      421 ttccagctcg cgtacaactc tatattaaat tagcaaaaaa tgccaataga gttaataaaa
      481 acctggtgtt acctgatgaa tatatcgctc tagagtttaa aggtatgagt ggcgcaggag
      541 ctatagaagt agacaaaaat ttgaccttca ggattcgtgg attaaataat attcatgtcc
      601 ttgactgctt tgttaatgtc gcactggaac cagctgatgg cgttgttgac tttggtaaaa
      661 taaattcccg aacaattaaa aataccagcg ttagtgagac gtttagtgta gtcatgacaa
      721 aagatcctgg tgcggcctgt actgagcaat tcaatatttt agggagcttt ttcactacgg
      781 atattttgag tgattatagc catctggata tgggtaatgg actgctattg aagatatttc
      841 ataaagatgg aacagcaacg gaatttaacc gcttctcaca atttgcttct ttttcatcgt
      901 ctagtgcacc ttcggtcacc gcaccattca gggcagaact tagtgcgaat ccggcagaga
      961 ctgttgttga gggaccgttt agtaaagacg tcatcctcaa aatcacctat aactagcatc
     1021 taatacaaac actaaaacgg gccatcaggc ccgttatatc agtgctctaa ctccagctct
     1081 cttgcctgca tcatctgttg acaataccag gcataacgtg cgcaaaacca ggttctatgt
     1141 tcttcatcca tgttaccagt aacggcaaga atatcgaccg ggcgaccttc tgcgtgcatt
     1201 ctggcaaaac tacgcgccag gaaatcgaaa ttatttaaag aattaagact atgatccata
     1261 agcacatcct ccggttatcg catacctttt aaggccatat gctttttctg atttcagtat
     1321 tccccaatca gtgtagtgcc tttaaacata acgtgcgtaa atatattgtc cccgatcaca
     1381 ctttttagtg aaaaataaaa aaccatatta aaaaactttc tcttccagaa cttctctaac
     1441 atgtgttatt attaacaaag ataaatgtaa gcaaggaaac tatcccaaag taaatattgc
     1501 gtcactaaat ggacattgga gtgacatatg attttattcg ccgactataa taccccttat
     1561 cttttcgcta tttcctttgt actgttgatc ggccttttag aaattttagc gcttatctgc
     1621 ggtcacatgc tctccggtgc tcttgatgca catcttgacc attatgattc cataaccaca
     1681 ggtcatatta gccaggcact tcattatctc aatatcggaa gattgccagc tctcgtcgtt
     1741 ctctgcttgc tggctggttt ttttggtctt attggcatcc tgttgcagca tgcctgcatc
     1801 atggtctggc aatcaccact gtcgaacctg ttcgtggttc ctgtcagttt actgtttacg
     1861 attattgcgg tgcattatac cggtaaaata gttgcgccct ggattccacg cgaccatagt
     1921 tcagctatta cagaagaaga atatattggt agcatggcat taattaccgg tcatcaagcc
     1981 acttcaggta atccctgcga aggaaaactc accgatcaat ttggccaaat acattattta
     2041 ctactggagc ctgaagaagg aaaatttttc actaaaggag acaaagtgtt aattatttgc
     2101 cgactttctg caacgcgata tttggcggaa aacaatccct ggccacaaat attgtaattt
     2161 attaatcaaa aggaaattta ataatggatg atattgttaa ttctgtgtcc tcctggatgt
     2221 ttaccgcgat tattgccgta tgcattctgt ttattattgg aattattttc gccaggctct
     2281 atcgtcgcgc ttcggcagag caagcttttg ttcgtactgg tttaggtggg caaaaagtgg
     2341 tgatgagcgg tggcgcaatc gtgatgccga tctttcatga aataatcccc atcaatatga
     2401 atactctgaa gctggaagtc agccgctcaa ccattgatag cctgattacg aaagatcgta
     2461 tgcgtgtcga tgtagtagtc gctttctttg tacgggtaaa accgtctgta gaaggcatcg
     2521 ccactgcggc gcagacgttg gggcaacgca cgctatcacc agaagactta cgtatgttgg
     2581 ttgaagataa atttgtcgat gccctccgtg caacagctgc acaaatgacc atgcatgagt
     2641 tacaggatac ccgcgagaac ttcgtgcagg gagtacaaaa tactgtcgca gaagacctgt
     2701 cgaaaaacgg tcttgagctg gaaagcgttt cacttaccaa ctttaaccag acctcgaaag
     2761 aacatttcaa tccgaacaat gcctttgacg ccgaaggttt aaccaaactg actcaggaga
     2821 cggagcgccg tcgtcgcgaa cgtaacgaag ttgaacagga tgtagaagtt gcggtgcgtg
     2881 agaaaaaccg tgatgcactt tcgcgcaagt tggagattga acaacaagaa gcgtttatga
     2941 cgcttgagca ggagcagcag gttaaaaccc gtactgccga acagaatgca cgtattgcgg
     3001 cttttgaagc tgaacgtcgt cgtgaagcag agcagacgcg aattctggct gaacgacaaa
     3061 ttcaggaaac ggagatcgag cgcgagcagg ccgtccgctc aagaaaggtt gaggctgaac
     3121 gtgaagttcg tattaaagag atcgaacaac agcaggtcac cgaaatcgcc aaccagacga
     3181 aatcgatcgc tattgccgcc aaatcggaac aacagtccca ggcagaagcg cgtgctaacc
     3241 ttgcactcgc agaagcagta agtgcgcaac aaaacgtaga aaccactcgc cagaccgcag
     3301 aagccgatcg tgctaaacaa gttgccctaa tcgctgccgc gcaggatgca gaaaccaaag
     3361 cggttgaact gaccgtgcgg gcgaaagcag aaaaagaagc cgcagagatg caggcggcgg
     3421 ctatcgttga gttagccgaa gctacacgta aaaagggtct ggcggaagca gaagcacaac
     3481 gtgcgctgaa cgatgctatc aacgtacttt ctgatgaaca aaccagcctt aaattcaaac
     3541 tggccttgtt gcaggcgcta cctgcggtaa tagaaaaatc cgttgagccg atgaaatcta
     3601 ttgacggtat caagattatt caggtcgatg gtctgaatcg tggcagcgct gcgggtgatg
     3661 caaacacggg taatgtgggg ggcggaaacc tggcggaaca agcattatca gccgctctct
     3721 cttaccgcac acaggcaccg ctgattgact ccttgctcaa tgaaattggc gtttcaggcg
     3781 gctcactggc ggcattgact tcacccttaa cctcaacaac tcccgtcgcc gaaaacgtag
     3841 aataaatttt cagctatgaa cgtccctggc acaaaaagcc ggggattttt tatatctgcg
     3901 ttccgctaaa aactgcaaat gatcaggtcg ttgcaacgtt ttgcgtgacc gctcggggaa
     3961 ggcaaaactg cctctgggaa agcgttgcgc gaggtccggc gctcatcaac aatcggggag
     4021 tcagcaaagg gctgaaacgg gaaagcccct cccgaggaag gggccttaca gaaggaaagg
     4081 gttatgatga agctcgtcat catactgatt gtgttgttac tcgtaagttt cgcagcttat
     4141 taacagccaa tcagaggggg gagaaatcct ccctcaccct tgttccttta ctttaggttg
     4201 aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg
     4261 attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc
     4321 gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc
     4381 atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc
     4441 cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttgtgt tgttactgtt
     4501 aagtttcccg acttactaac aactgatcag aggggggaga aatcctccct tacccttgtt
     4561 cctttacttt aggttgaaaa aacaacagcg tcaataggcc tgcctgctac gaagcgagat
     4621 ctgtgaaccg ctttccggtt agcctttttt atcctgttgg atcttcttga tgatgttagt
     4681 ggtcgagcaa ccgtcttcga agttgagcac cagcacttcg ccgccgttgg cccagacttc
     4741 tttactcccg gcaatttctt ctggtttata gtcgccgcct ttcaccagca gatctggcag
     4801 gatcccggcg atcagacgct gcggcgtgtc ttcttcaaac gacactaccc agtcgaccgc
     4861 ttccagtgcg cccagcacaa tcatacgctg ctcgagtggg tttaccgggc gggaatcccc
     4921 tttcagccgt ttggtggagg catcgctgtt cacagcgaca atcagacggt cacccagttt
     4981 gcgagcattt gccagatagg agacgtgccc ggcgtgcagg atgtcaaaga caccgttggt
     5041 cattaccacc ttttcgccgc gtttacgcgc tgccgctaca gcaagcttca gttcctcttc
     5101 ggtcatcaca ccaaagccgg tatctgcgcg cccacgcact gcattttcca gctcgatcgg
     5161 cgaaaccgtg gaggttccca gtttgccgac caccacgcca gccgccgcat tggcaaagaa
     5221 gcaggcttct tccagcgaat tacccgctgc cagcgttgcc gccaggacgc caatcaccgt
     5281 gtcgcccgca ccggtaacgt catacacttc ctgcgcctgg gttggcatat gcagcggcgc
     5341 tttacccggt tgcagcagcg acataccctg ttcggaacgg gtcactaaca gagccgagag
     5401 ttcgtaatcg gcaatcagtt tcatgccgcg ctcaacaatc tcttcttcgg tcttacattt
     5461 accgacaaca gcttcaaatt ccgagagatt cggcgttaac agcgtagcgc cgcggtagcg
     5521 ctcaaaatcg gtaccttttg gatcaatcag caccggaacg cccgctttac gcgccagttg
     5581 gatcatctgc tgtacgcttg ccagcgcacc tttggcgtag tcagaaagca ccagcgcgcc
     5641 aatcgaactc agcgcctgat taatccgctc gtgcagcggc tgcggatcaa caccttcgaa
     5701 accttcttca aaatccaggc ggatcagctg ttggttgcgg gaaagtaccc gtaatttggt
     5761 gatagtcgga tgcgtcggta cagaaacgaa gtcgcatttg acgttgacgt cggccagaga
     5821 tttactcagc gcacgcgccg catcgtcaat gcccgtcaac ccgaccaggc gtgcattagc
     5881 accgagagaa gcgatattca tcgccacgtt agccgcgccg cccggacgtt cttcgatggt
     5941 attgacttta accacgggca ccggcgcttc cggcgagata cggctggtgg gaccgtacca
     6001 gtaacgatcc agcatcacat caccaaccac catcactcct gcacgttcaa actctggcag
     6061 cgttactttc attcctgtct cctgagagat tcaaaatttg cgcgcgataa taccatactt
     6121 cattcttcca ccagccactt ctgccagctt gcccgcacca gttcacgctc tgcggtgaag
     6181 caatcctccg gcacatggcc cggcaattcc tgtaatgcca gatgatgaag ttcatcgcgc
     6241 agcgtagtat acgcacgggt cagcgccatc gcttcctgct cttccataat gtcgttttgc
     6301 gccagtagtt ccagaatgcg cacgttgtct gaccagcgcg ttaacttcgg tttttcatga
     6361 gcgtagcgca acaccagata ttgggtaata aattcgatat cggtaattcc gccttcatca
     6421 gctttgatat caaagcgatc gcgatgttta ttgccgagat gagcgcgcat tttctcgcgc
     6481 atttcccgca cttccgtttg cagagtttta ccttcacgcg gcagcgtcat aatctcgcga
     6541 cgcaccgcgt caaagtgcgc ggtgagctgc ggatcgccgt acactacccg cgcacgcacc
     6601 agcgcctgat gttcccacgt ccaggcctcg tttttctgat aatcggcaaa tgcttctgcg
     6661 gatgtcacca gcattcccgc ggccccggac gggcgtagcc gtgcatccac ttcatacaaa
     6721 atgccggaag aggtacgcgt actgaacaga tgcataatgc gttgcgccag acgcagataa
     6781 aactgtcgcc cgtcgatttc ccgctcgccg tcagtcatcg catccattgg gcaatcatgg
     6841 aggaagatca ggtcaagatc ggaactgtag cctaactccc agccgcccag cttgccgtag
     6901 ccgaccaccg caaaaccacg gccttcgcga tcgttgaggt gatttggctt accgtagcgg
     6961 gccaccattt gaacccaagc ctgctgaacg acagcatcta tcatagcctc tgccagccag
     7021 gttaagtgat cgctcacttt catcaccggt agcgtaccag cgatatccgc tgcggcgatg
     7081 cgtaacagct gtgcctgttt gaactgacgc agcgcctcaa gctgttgctc ttcgtcatct
     7141 tccggcacgc gcagcaaata ctggcgcaac tcatcgcggt aggcatcggt cgccgtcggc
     7201 tggtaaaggg tgtttggatc gagcaattca tccagcaata atggataacg cgccagttgg
     7261 ctggcaatca tcggcgacgc ggcacacaga gaaatcaaat gtttaagcgc cgcggggaat
     7321 tcactgagca attctaaata ggtggtgcgg gtaacaatcc ccaccagcaa ggcggtaatg
     7381 cgcgacagcg taacggcagc gtcttcacgc gcacagacat cacttagcag atgcggcatc
     7441 agatggtcga gcacctgacg tcctcgcggc ccgatggtgc gcttatccag ctctttgcgg
     7501 aaatcggcaa tcagcgttag cacctgtttg cgatcatcct cgctaagatg cgccagcact
     7561 ggcgtagtgt catcttcctg caacgcatcc tgccacagct cacgccactg ttccgacagc
     7621 gactcttcct gagtttcact ttcgtcgtcg ccaatcaatt cattaaacac ccggcgcaca
     7681 ttggtcatat gtgcggtcag cgccccggtc agttgcggcc agtcagcaaa gtccatcgcc
     7741 cacgccagcc gcgcacggtt aagctcatca gaaggaagcg tctgggtttg ttcgtcgtta
     7801 atgctttgca gcaggttttc cagacgccgc aggaacagat acgccactcg caattgttca
     7861 gcatcgtttt cagaaagcag atgcagcgcg gcaatgacgc tgagcgttgg cagtaaagag
     7921 tgcgactgca gcgacggttc gcgcccaccg cgaatgagct ggaacacctg aacgataaat
     7981 tcaatttcgc gaatgccgcc tgcgccgagt ttgatattgt cggtcaaacc acgtcgacgc
     8041 acttcacggg caatcatccc tttcatgttg cgcagcgact gaatcacgct gaaatcgatg
     8101 taacgacgga aaacaaacgg gcgcagcatc gcacgcaact cgttagcata gacgccttcg
     8161 ctatcgccca taatccgcgc cttgaccatc gcgtaacgct cccagtcgcg cccctgctcc
     8221 tggtaataat cctccaacgc ggcaaagctc agcaccagcg ggccactttc gccaaacgga
     8281 cgcagccgca tatccacgcg atagacgaag ccatcctgcg ttggttgatc caacacttta
     8341 atcagccgct gccccatgcg ggtaaaaaac tgcgcgttat ccagttcccg gcgtccgccc
     8401 tgcgtacaac catgttccgg ccaggcaaaa atcagatcga tatctgagga gaaattcagt
     8461 tccccaccgc ccagcttacc catgcctaaa atcagcagcg gttgcgcttc gccctgcgca
     8521 ttgcacggcg ttccccactc gcggcagcaa gcgtcataca gccagtcacg cgcggcgaca
     8581 atcagcgtct ccgccagatg gctgagctgc tgcaatatgc tctcttcagt aaccaaagcc
     8641 agcgtttgcg cccaggcgat gcgcaccata atgcgccgcc ggaatagccg cagctcgcgc
     8701 attaacccgg cttcgtcact cacattgctg agcgcctcct gcaaccatgc cgcgtaatgc
     8761 tgccattcgt cggcctgcgg cggttggctt tccagttccg tcagccactc tggatgcaca
     8821 atcacgctgt cctgcacaaa atcactaaaa gtaagtactg acttcgcctg tgcgctaagt
     8881 gattcctcgg ctaaaggctc tggcagccgc tcaacaacgg tctgccagta ctgctgtaac
     8941 ggtgaagaga gcggcttcat aagcgatttt atccttgcct gattaacgtt ttccgctgtg
     9001 caaccagaac ggttcctgat tgtttgcctc attacggaaa tgttcaattt caatgcgttg
     9061 cccggtcgca atagcgtgac gcagcccctg ccagttctcc agccaggctt gcgcgacgac
     9121 aggatcatag taacctgcca gcagcagtat tgagtcaata tcacgcgtca ggcgtggcaa
     9181 ctgatcatgg tagcgatcgc ctaacggctg gcagaaaacg cttttcagtt cagcggcatg
     9241 gcgggaaaga tggatatcgg caaagcgttt gaaggaatcg ctgattttac cctgcgcttt
     9301 ggcatctaaa aacggctgcc aggctttgct taccaaccat tcggtcaacg ccagctttgc
     9361 cattgccgtt tcggtagagt agaccgccgt cacggcagaa accgcagaag cgatggtcgc
     9421 ttcgcattga gtcagcaaat cacgtaagtg agtgctcgct ttacgcggca caatgccacc
     9481 gaacagcatc agtgcatggc gaacaaggcc aatggctgcc agcacctgct ctttcgccgc
     9541 atcgttgccg cgtacccaca gttcttcatg atactgccat tgcgctaacg ccagctcgaa
     9601 cgccgcttcc agcccctgtt ccacatcggc ttttgccgca acatgcaaaa tggtggtcgg
     9661 tttgatttca cgcgccggat tgccctgcgc cagatgatag ccacgcgccg ctttgctcag
     9721 gctgccctgg cgtaatccgg tttgcgatac cagttggttc gccagtttca gcaccgcgcg
     9781 cgtgtcgccg ctaagcagtt ccagttccag ctcacagata ggttcagcaa attcccccgc
     9841 tttcacctcc ccctgatcga gagcgatttc aattcggctg tcatcgaccg ccaccagcca
     9901 tttttcgcga taaaaatcgg tgctgaacag cggttgtacg cgggaagcga gatcggcggg
     9961 caattcgccg ttcggccaga cttccgtcgg taactgcgcc aggtcgagcg tcggttcgct
    10021 caacgccacg ttatattccg ggcgctggtg taagccgcct gtcactcttc ctgcaacttt
    10081 catggtcatc tcatagcgac cgttttcgcc acgaatacgt aagcccatat cgtgcccacg
    10141 cagccagtta tccggcgttt cgtagtaaat attcagcaac tgcacggggt catggtgctc
    10201 gccgcccagc gtattgagat ggtcacgcaa cgcctcaacg gcactgtgat taacaataaa
    10261 ctttaattcg atttcctgag ccatggcctt tacttatcgg ttatgtcaca taagtgacga
    10321 tgaacggcga acttaatgcg atcttttttg tcagtagata gtattttgcg ccaaattgcc
    10381 atgcaacggg caatttgacg ggcgtaaaag tttgaagcag tggcaagacc tgacacagag
    10441 gattccgatt gatgtcgaat gctttgcgta gcgacactga tgccactact atcgttccac
    10501 tttccatgac aataacgaca gcctgatgcc aaaattacgc ctgatcggat taactttact
    10561 cgcacttagc gccactgccg tctcacacgc tgaagaaacg cgctatgttt ctgatgaact
    10621 gaatacctgg gtccgtagcg gtccgggaga tcattatcgc ctcgtgggca cggttaacgc
    10681 cggcgaggaa gtgaccttat tacaaactga cgccaacacc aattatgccc aggtgaaaga
    10741 cagctctggc cgtaccgcct ggatcccgtt gaaacaactt agcactgagc caagcctgcg
    10801 ctcccgtgtg ccagatctgg aaaatcaggt caaaaccctg accgataaac tcaccaatat
    10861 cgataacacc tggaatcagc gcaccgcaga aatgcagcaa aaagtggcgc agagcgacag
    10921 cgtgatcaac ggattaaaag aagaaaatca gaaattgaaa aacgagctga ttgtcgcgca
    10981 gaaaaaggtc gatgccgcca gcgtacagtt ggatgacaaa cagcgcacca tcatcatgca
    11041 atggtttatg tatggcggtg gcgtgctggg gcttggcttg ctgctcggtc tggtactgcc
    11101 acacctgatc ccaacgcgca aacgcaaaga tcgctggatg aactaaatcg ccttcttcgt
    11161 tgcactgtct tattattagg aattgtgtat ttgacgaagg gatgatggcg tgaagattta
    11221 tctggtcggt ggtgctgttc gggatgcatt gttagggcta ccggtcaaag acagagattg
    11281 ggtggtggtc ggcagtacgc cacaggagat gctcgacgcg ggctaccagc aggtaggccg
    11341 cgattttcct gtgtttctgc atccgcaaac gcatgaagag tatgcgctgg cacgtaccga
    11401 acggaaatcc ggttccggtt acaccggttt tacttgctat gccgcaccgg atgtcacgct
    11461 ggaagatgat cttaagcgtc gcgatctgac cattaatgcg ctggcccagg acgataacgg
    11521 cgagattatc gacccgtaca acggtctggg cgatctgcaa aatcgtctgt tgcgccatgt
    11581 ttcccccgct tttggcgaag atccgttacg cgttctgcgc gtggcgcgtt ttgctgcgcg
    11641 ttatgcccac ctcggttttc gtattgccga tgaaactctg acgctgatgc gcgagatgac
    11701 ccatgcgggt gaactggaac acctgacgcc tgaacgggta tggaaagaga cggaaagcgc
    11761 cctcaccacc cgcaatccac aggtgttctt ccaggtactg cgcgattgtg gcgcactgcg
    11821 cgttttattc ccggaaattg acgcactgtt tggcgttccg gcccctgcca ggtggcatcc
    11881 ggaaatcgac acgggtattc ataccttaat gacgctctca atggcggcga tgctgagtcc
    11941 gcaggtcgat gtccgtttcg caactttatg ccacgatctc ggtaaaggac tgacgccgcc
    12001 agaactttgg ccgcgtcatc atggtcacgg tccggcgggt gtgaaattag tggaacaact
    12061 ctgccagcgt ctgcgcgtgc caaatgaaat tcgcgattta gccagactgg tggctgagtt
    12121 tcacgatctc atccacacct tcccaatgct gaacccaaaa actatcgtca aattgtttga
    12181 ttccatcgat gcctggcgta aaccgcagcg tgtcgagcaa ctggcgctga ccagcgaggc
    12241 tgacgtgcgc ggcagaaccg gttttgaatc agcggactac ccgcaaggcc gttggttgcg
    12301 cgaagcctgg gaagtggcgc agtcagtgcc gacaaaagcg gtcgttgaag cgggatttaa
    12361 aggtgtggag attcgcgagg agctgacccg acggcggatt gcggcgatag ccagttggaa
    12421 ggaacaacgt tgcccaaagc ctgactgagt ataaaactaa acgttaacac gaaccatccc
    12481 ctcgcccctt cggggagagg gttagggtga ggggaacaga ccagcaccgg tgcgaacaat
    12541 cacccccacc ccggccctct ccctacagag cgagggagaa aaccaggcct gctcccgaac
    12601 cttttctcaa tcaaaagaac acgacgtaca ccgcagccgc cacaataaag cgataaatgg
    12661 cgaacgggat aaacgaaatg cgcttaatca attgcaggaa ggttttaatc gctatcagcg
    12721 ccaccacaaa agcggtgata aacccaacgg caaacatcgg gatatcgccg gttgtcagga
    12781 agccccagct tttgtagaga tcgagcgccg ttgcgcccat catcatcggt accgccagca
    12841 ggaacgagaa ctcggaagca gcgtaacggc tcacgcccat cagcatccca cctgaaatgg
    12901 tggccccgga acgggagaaa cccggccaca gcgccagaca ctggaaacag ccaatcataa
    12961 acgcctgacg ataggtcata tcatcaagac ccggcgcacg cggctctttc ggcttcaggc
    13021 attcggcggc aatcagcaac aaaccgccaa cgaccagcgc atacatcaca tttatcgggt
    13081 taaacaatga cttaatcgtg tcgtggaaca atagccccag taccaccgcc ggaatcatcc
    13141 ccagcaaaat gtggatcagc gttaaacgac ctttgctttc accttcgtgc tgcaacgggc
    13201 ggccaaagtg gatgccaatc aggccaaaca gacgccgcca gaacatcact actaccgcca
    13261 gaattgatcc taactggatc acaacttcaa aggttttcgc cgtgtcgccc tcaaacccca
    13321 acaagtgacc gacaataatc atatggcccg tgctggatac cggcagaaat tctgtcaatc
    13381 cttcgaccac acccaatatt gccgctatca gcagcgagtg catatcgctc atcaataaac
    13441 ccctaaatta ttaaaatgta ccgcttgtcc gaactactgc gtatgaccag gttataaccg
    13501 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    13561 tacgccaaca ttcgccgccc gcgccactgc gcctggcttg ctgagtttga tacgcaccca
    13621 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    13681 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    13741 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    13801 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    13861 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    13921 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    13981 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    14041 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    14101 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    14161 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    14221 taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    14281 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    14341 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    14401 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtat
    14461 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    14521 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    14581 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    14641 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    14701 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    14761 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    14821 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    14881 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    14941 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    15001 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    15061 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    15121 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    15181 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    15241 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttgcgtgacg
    15301 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    15361 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    15421 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tttgcccaag cgtcgccgct
    15481 gcagcactaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    15541 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    15601 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    15661 ctgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    15721 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    15781 taagcaacag gcagtgaata agttgacaga gattgtcgct aactttaccg ccatgatttc
    15841 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    15901 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    15961 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    16021 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    16081 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    16141 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcaga
    16201 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    16261 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    16321 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    16381 cgccgtactc tcgcgtaaag ccattttgcg tccgattggc agccgccatc ccaatccaaa
    16441 agcggcagag ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    16501 agggctgacc ggcaacagtt cagtgatggg cgtgcatatc gaatctgccg cccgccatcc
    16561 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacgctgct
    16621 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    16681 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg cgatgtgatc
    16741 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    16801 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    16861 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    16921 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    16981 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    17041 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    17101 acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt cggcccgctg
    17161 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    17221 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    17281 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    17341 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    17401 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    17461 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat ctccatcatc
    17521 gccatactct ctccctggct gctgttcagc cccgaacagc tcgcccagcc aggctttaaa
    17581 ttcactgcaa aatccctctc gtgggcggtt tccggttttt ctaattcggt tatctggctg
    17641 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggctggg acgccgcatt
    17701 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    17761 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    17821 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    17881 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    17941 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    18001 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    18061 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    18121 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    18181 gaaaaacgga tgctggggct gatggtaggc gcgctggtgc tgtggatttt cggcggtgat
    18241 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    18301 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    18361 tcgcttatca ccctcgctac cggactcaac aacactggtt ttattagctg gtttggcaaa
    18421 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    18481 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    18541 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    18601 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    18661 ccgatttact acggtagtgg ttatctgcca acggtggatt actggcgact gggggcgatt
    18721 tttggactga tattcctcgt gttgctggtg attaccggct tgctgtggat gcccgtggtg
    18781 ttgctttaag tgaggacggt gccggaaatt gctctccggc accggatagt tttacgcagc
    18841 cggtaactct gccagtggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    18901 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaattc
    18961 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttccgcca gcttcgcccg
    19021 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    19081 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    19141 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    19201 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    19261 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    19321 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    19381 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    19441 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    19501 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    19561 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    19621 agcatcaatg tcttttgccg ttaaaccaga ctcctttagc gccgcctgga tcaacggtac
    19681 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccgt agtcagcgtg
    19741 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    19801 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    19861 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    19921 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa aggccgcggt
    19981 gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc attttgaaat aagctggcgt
    20041 tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc acatgccggt aattaaagta
    20101 cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca agcgttcctg cgaaaaagca
    20161 ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa aaccgactac cgaacgtaag
    20221 cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac tggctcgcga aaacgcacgc
    20281 cgcactcgtc tgtactaatt ccccgagagc gttgctctcc gatcagaccg agttgtagtt
    20341 gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc gtttatgaat tgctaaaaat
    20401 cggggcctat ggctggacga atcccacgcg tattcattaa tgatctgctg gcacgcactg
    20461 acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa gcagggcaag aatttccacg
    20521 cgtgttgtcc attccacaac gagaaaaccc cgtcattcac cgttaacggt gagaaacagt
    20581 tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat cgacttcctg atgaactacg
    20641 acaagctcga gttcgttgaa acggtcgaag agctggcagc aatgcacaat cttgaagtgc
    20701 catttgaagc aggcagcggc cccagccaga tcgagcgcca tcagaggcaa acgctttatc
    20761 agttgatgga cggtctgaat acgttttacc aacaatcttt acaacaacct gttgccacgt
    20821 ctgcgcgcca gtatctggaa aaacgcggat taagccacga ggttatcgct cgctttgcga
    20881 ttggttttgc gccccccggc tgggacaacg tcctgaagcg gtttggcggc aatccagaaa
    20941 atcgccagtc attgattgat gcggggatgt tggtcactaa cgatcaggga cgcagttacg
    21001 atcgtttccg cgagcgggtg atgttcccca ttcgcgataa acgcggtcgg gtgattggtt
    21061 ttggcgggcg cgtgctgggc aacgataccc ccaaatacct gaactcgccg gaaacagaca
    21121 ttttccataa aggccgccag ctttacggtc tttatgaagc gcagcaggat aacgctgaac
    21181 ccaatcgtct gcttgtggtc gaaggctata tggacgcggt ggcgctggcg caatacggca
    21241 ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc cgatcacata caactgttgt
    21301 tccgcgcgac caacaatgtc atttgctgtt atgacggcga ccgtgcaggc cgcgatgccg
    21361 catggcgagc gctggaaacg gcgctgcctt acatgacaga cggccgtcag ctacgcttta
    21421 tgtttttgcc ggatggcgaa gaccctgaca cgctggtacg aaaagaaggt aaagaagcgt
    21481 ttgaagcgcg gatggagcag gcgatgccac tctccgcatt tctgtttaac agcctgatgc
    21541 cgcaagttga tctgagtacc cctgacgggc gcgcacgttt gagtacgctg gcactgccat
    21601 tgatatcgca agtgccgggc gaaacgctgc gaatatatct tcgtcaggaa ttaggcaaca
    21661 aattaggcat acttgatgac agccagcttg aacgattaat gccaaaagcg gcagagagcg
    21721 gcgtttctcg ccctgttccg cagctaaaac gcacgaccat gcgtatactt atagggttgc
    21781 tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct tgagaatctg gatgaaaata
    21841 agctccctgg acttggctta ttcagagaac tggtcaacac ttgtctctcc cagccaggtc
    21901 tgaccaccgg gcaactttta gagcactatc gtggtacaaa taatgctgcc acccttgaaa
    21961 aactgtcgat gtgggacgat atagcagata agaatattgc tgagcaaacc ttcaccgact
    22021 cactcaacca tatgtttgat tcgctgcttg aactgcgcca ggaagagtta atcgctcgtg
    22081 agcgcacgca tggtttaagc aacgaagaac gcctggagct ctggacatta aaccaggagc
    22141 tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg atcgggaagc ccccgacagc
    22201 cgcactgaga ggcagcggca aatatataag tacgccctcg taattatcgt tggcggtaaa
    22261 caaccgttgg atttcagcgt taacggctga aggacatcgg gtcaatcgcc caacaccaac
    22321 ctcatgaaat aagtgtggat accgtcttat ggagcaaaac ccgcagtcac agctgaaact
    22381 tcttgtcacc cgtggtaagg agcaaggcta tctgacctat gccgaggtca atgaccatct
    22441 gccggaagat atcgtcgatt cagatcagat cgaagacatc atccaaatga tcaacgacat
    22501 gggcattcag gtgatggaag aagcaccgga tgccgatgat ctgatgctgg ctgaaaacac
    22561 cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt tccagcgtgg aatctgaaat
    22621 cgggcgcacg actgacccgg tacgcatgta catgcgtgaa atgggcaccg ttgaactgtt
    22681 gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa gacgggatca accaggttca
    22741 atgctccgtt gctgaatatc cggaagcgat cacctatctg ctggaacagt acgatcgtgt
    22801 tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc tttgttgacc cgaacgcaga
    22861 agaagatctg gcacctaccg ccactcacgt cggttctgag ctttcccagg aagatctgga
    22921 cgatgacgaa gatgaagacg aagaagatgg cgatgacgac agcgccgatg atgacaacag
    22981 catcgacccg gaactggctc gcgaaaaatt tgcggaacta cgcgctcagt acgttgtaac
    23041 gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc gctcaggaag agatcctgaa
    23101 actgtctgaa gtattcaaac agttccgcct ggtgccgaag cagtttgact acctggtcaa
    23161 cagcatgcgc gtcatgatgg accgcgttcg tacgcaagaa cgtctgatca tgaagctctg
    23221 cgttgagcag tgcaaaatgc cgaagaaaaa cttcattacc ctgtttaccg gcaacgaaac
    23281 cagcgatacc tggttcaacg cggcaattgc gatgaacaag ccgtggtcgg aaaaactgca
    23341 cgatgtctct gaagaagtgc atcgcgccct gcagaaactg cagcagattg aagaagaaac
    23401 cggcctgacc atcgagcagg ttaaagatat caaccgtcgt atgtccatcg gtgaagcgaa
    23461 agcccgccgt gcgaagaaag agatggttga agcgaactta cgtctggtta tttctatcgc
    23521 caagaaatac accaaccgtg gcttgcagtt ccttgacctg attcaggaag gcaacatcgg
    23581 tctgatgaaa gcggttgata aattcgaata ccgccgtggt tacaagttct ccacctacgc
    23641 aacctggtgg atccgtcagg cgatcacccg ttctatcgcg gatcaggcgc gcaccatccg
    23701 tattccggtg catatgattg agactatcaa caaactcaac cgtatttctc gccagatgct
    23761 gcaagagatg ggccgtgaac cgacgccgga agaactggct gaacgtatgc tgatgccgga
    23821 agacaagatc cgcaaagtgc tgaagatcgc caaagagcca atctccatgg aaacgccgat
    23881 cggtgatgat gaagattcgc atctggggga tttcatcgag gataccaccc tcgagctgcc
    23941 gctggattct gcgaccaccg aaagcctgcg tgcggcaacg cacgacgtgc tggctggcct
    24001 gaccgcgcgt gaagcgaaag ttctgcgtat gcgtttcggt atcgatatga acaccgacca
    24061 cacgctggaa gaagtgggta aacagttcga cgttacccgc gaacgtatcc gtcagatcga
    24121 agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct gaagtgttgc gtagcttcct
    24181 ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca cccggcacta gtccctttgc
    24241 acaaacgcca ccttttcggt ggcgtttttt atcgcccacg cactaccagc gcctggtcca
    24301 gctcgcgata cgcttcaacc agtttctcta gtgaaacgcg acttaaaccg ctgggatttg
    24361 gcagcaccca aatctgcgtc gaaccaatgg tgagcgtttg tttcccccac tgtgcaccgc
    24421 gctggctgaa tccctgttca tatgctcgtt tgcccagaat cgccaacgcc tgcggctgat
    24481 aatcttcaat tttttcaatc agcttacgcc cacctgcgtg cagctcctgc tttgaaactt
    24541 cattggcttg caccgttgga cggtctacca gtttggtgac gccacaacga taatccagca
    24601 gatgctgtgc ctcctgcggc ttcaactgac ggtcggtaaa cccggcctga tatatcacct
    24661 tccagaagcg atttgctgga tgagcaaagg gaaaaccagt cccggcggat gaaagcccag
    24721 ggttgatacc gcaaaacacg acccgtaacc ctggagccaa aatatcctca accatattta
    24781 ctcctttcta acatcaccag gaaaagtata acggattgaa aatacattgt ttataaaaac
    24841 agcaggcgca cggtaatggc tggattgcga cacggagtta ctttataatc cgctaccatg
    24901 gccccttagc tcagtggtta gagcaggcga ctcataatcg cttggtcgct ggttcaagtc
    24961 cagcaggggc caccagatat agcaaaggct gacgagaaat cgtcagcctt tttcttttta
    25021 tatatcagtt actttgcgtg ccagtaagcc gctgcacgta cccgctgtgg gtcatactgt
    25081 tccgcttcaa agcggcggct taaattctta acgactttac cttcgccggt tatccagatg
    25141 aagtaatcat cggcagggat ttgcatctgc gccagacgcg catctaccgc ctgctcatca
    25201 tgtgccagcc attcgatatt aaaaccatca aggtgcgcga gataatcctg acaggcgtta
    25261 tcccgcacgc taaccagcgc actaacttgc ggtttaacgg caagtttgct caacgtttcc
    25321 aggcggcggc gcaatgcggg cattccggat tcatcgcaga catacagctg atacgcgtaa
    25381 tcttccggca ccaccagcga accgcgcgga cctgccaccg taagtttatc gcccggttgc
    25441 gcctgcatcg cccagccgct ggcgacccca ccgtcgtgaa taaagaaatc aatcgccagt
    25501 tcatggcgta gttcgtcata cagcggcgta tagtcacgcg acggtgggcg tggtccttcc
    25561 ggccagacga tgccctcttc cgttaccgtt ggcggcacaa agtgagcgtc aggttgagga
    25621 aagaagagtt tgctgtgatc gtcaaagccg cgcgatgtaa aaccgtccag cgcctcgccg
    25681 ccaaggacaa tgcgctgaaa accggcgctg atgcgctcaa cgcgtaacac agtcagttca
    25741 cggaagcgca gatcattgcg aacgcgctgc gggtagcggg gggagttatt cattgttatc
    25801 gccttcgtga tagtaatcag atatatctaa ataattttcg taaatgataa tgattgttaa
    25861 tcatgataaa tgcaagcgat ttgtagaact gatatatctg aagtatgtga aggcattcag
    25921 tgccatagca atgctgccgg atgcggcgtg aacgtcttat ccggcctgga tgtgaggtgc
    25981 ttccacgttt aaaaatcatt tttgtacttg cagtaatgct cacatcagat ataaattaga
    26041 tatatctaat caagcaaaag gaggctgata tgagccatca tcacgaaggg tgttgtaaac
    26101 atgaaggcca gccacgccat gaggactgct gcaaaggtga gaagtcagaa cacgagcact
    26161 gcggacacgg tcaccagcat gaacacggtc aatgctgcgg tggtcgccac ggtcgcggcg
    26221 gcggtcgtcg gcaacgtttc tttggtcacg gtgaattgcg tctggtgatt ctggatattc
    26281 tctcgcgcga tgacagccac ggttacgaat tgattaaaac gattgagaac ctaacccagg
    26341 ggaattacac cccaagcccg ggcgtcatct acccaacgct ggattttctg caggagcagt
    26401 cgctgattac catccgcgaa gaggaaggtg gtaagaagca gattgcgctg accgaacaag
    26461 gcgcgcagtg gctggaagaa aaccgcgaac aggtggagat gattgaagaa cgcatcaaag
    26521 cgcgttgcgt tggcgcggcg ctgcgccaga acccgcaaat gaagcgggcg ctggataatt
    26581 ttaaagcggt gctggattta cgcgtcaacc agagcgatat cagtgatgca caaataaaaa
    26641 agatcattgc ggtgatcgac cgcgccgctt ttgatattac gcaactggat taatcgccgc
    26701 atccgccagt ggcgcggtgc aattgccgga tgcgacgctt gacgcgcctt atccggccta
    26761 cacccgctac acaccccgca ggcctgataa gatgcgccag catcgcatca ggcattgtgc
    26821 tccaaccgcc ggatccggca taccgattaa tgcagcaccg tcaccgcgtc ttccagtcgg
    26881 ctggcgcggt gtttcaccat cgccgacacc tgcgcactct cttccaccag ctcggcattt
    26941 ttctgggtga tcaggttaag ctcatccact gcacgggtca ggctggaaag cccatcggcc
    27001 tgttccagcg ttgaatggct aatctgggcg atcaactggg tgacgttttt cacctgtgcc
    27061 acaatatctt ccatcgtccg tccggcggcg tgtacctgct gcgaaccgga ttgcacctta
    27121 tcagcactgg catcaatcag cttgcgaata tcgttggcag cattagcgct gcggctggct
    27181 aaatgacgca cttcccctgc caccactgca aaacctttgc cctgttcgcc ggcacgcgcc
    27241 gcttccaccg ccgcattcag ggccagaata ttggtctgaa acgcaatatc gttaatcagc
    27301 gaagtaatgg tgccaatgcg ctgggtactg tcggcgatat cgtccattgt cttgatcacc
    27361 gtggtcatcg cctccccacc ctgcactgcc gcattactgg cggtgataga aagtttatcg
    27421 gcagccgacg ccgtggcaga gttctgtttc accgacgccg ccatttggtt catggtcgcc
    27481 actgtttgct gaacgttatc aactgtctgc tgggtatgtt cgttcagttc atcggtgcct
    27541 ttcgccagcg tctcactgcc atttctgaca ctggacacct ggcttgagac atcgttaatt
    27601 agccaacggc acatcaggcc aagctgccct accgcgcgta atgtcagccc cagctcatcg
    27661 ctgcgattca ggtgctcaac actattacgc tctccggtcg ccaccttcag tgcctgacgg
    27721 gcgacatttt ctatcgggcg cacaatctgc cattcaaaac aagtgcttgc caacaatacc
    27781 actaacacac agaggaaata cgtcaccacc ggggcagcaa caaaccaaag catggccgcc
    27841 agcaagataa acatcagggt catcactcca cgcgcccgcc agcgaagcgg taatgaaggc
    27901 agtttaccca gccagccttt acgcaccacc aggcctttat gaatacgctt actggtacgt
    27961 ccggcgttca gcgctttgta cagcggctcc accgccgcaa tctcttcatc cgtcgcccgg
    28021 gtacgaatcg acatatagcc actgattttt ccctcgcgca ccatcggtac cgcattggcc
    28081 cgcacccaat aatggtcgcc atttttgcgg cgatttttca cgatgccgct ccagggctcc
    28141 ccttttttca gggtgaacca catatccgca aacgccgctt ttggcatatc cgggtgacgc
    28201 accatgttgt gcggctgccc ttgtaactct tgcaaggtaa agccgctcac ctgcacaaaa
    28261 gtgtcattag catgagtgat atagctttgc agatcggtag tggacatcag agtggtatcg
    28321 tccgccagcg gggtattttg ctgggtgaca tacggatgag aagacatgtt cgcgtcctgt
    28381 gcaggttata tggttgttaa cttcttgtca gagtttatgt cggccccgct gcggttatct
    28441 ttaaccgatt aatttgattt agatcgcaat ttgcg
//
LOCUS       DAZFRN010000053        28381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFRN010000053 DAZFRN010000000
VERSION     DAZFRN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.053"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(51..581)
                     /locus_tag="ACHKUB_002214"
     CDS             complement(51..581)
                     /locus_tag="ACHKUB_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005285503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655226.1"
                     /translation="MKNANRVVINLFSISLVTMISGCASYWGKVPYNPPSDTEKNIAH
                     VRLIGNPPGFTISQYGNKPAAVDPSNMVILRPTHDLGFPKVNNDPERYKETYYDTNVY
                     AGTPTRIGFKFLHGCEDVGYTFLPEKGETYEFRLSVTDKTGYCVLYASHLKYDSENKL
                     YITENIEEYKGYVYRE"
     gene            1160..1402
                     /locus_tag="ACHKUB_002215"
     CDS             1160..1402
                     /locus_tag="ACHKUB_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655227.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFSSFAKKVICSVLPDTQPSLDAFV
                     NSKNSEEHLNFSSDSTFPSCPDINNINNINNINNYY"
     gene            complement(1465..2973)
                     /locus_tag="ACHKUB_002216"
     CDS             complement(1465..2973)
                     /locus_tag="ACHKUB_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO6655228.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     IWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVHAGVIASGIQRGTETVSGAAQTAGTIARSGVKSK"
     gene            complement(2980..3321)
                     /locus_tag="ACHKUB_002217"
     CDS             complement(2980..3321)
                     /locus_tag="ACHKUB_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655229.1"
                     /translation="MSRIYDRVKKRMKVFALYTLLLTGITVFSLWLADTGMRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            complement(3326..4747)
                     /locus_tag="ACHKUB_002218"
     CDS             complement(3326..4747)
                     /locus_tag="ACHKUB_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO6655230.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            complement(4761..5735)
                     /locus_tag="ACHKUB_002219"
     CDS             complement(4761..5735)
                     /locus_tag="ACHKUB_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO6655231.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCTVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            complement(5732..6127)
                     /locus_tag="ACHKUB_002220"
     CDS             complement(5732..6127)
                     /locus_tag="ACHKUB_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO6655232.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNSPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            complement(6263..6895)
                     /locus_tag="ACHKUB_002221"
     CDS             complement(6263..6895)
                     /locus_tag="ACHKUB_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6655233.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            complement(7087..7536)
                     /locus_tag="ACHKUB_002222"
     CDS             complement(7087..7536)
                     /locus_tag="ACHKUB_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655234.1"
                     /translation="MLKKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQLS
                     DVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKVAEVSEAAQSGLS
                     EVKDAVEEVKTQAAGKVAEVSEATQSGLSDVKDTAPEKGAEISVTEE"
     gene            complement(7922..8110)
                     /locus_tag="ACHKUB_002223"
     CDS             complement(7922..8110)
                     /locus_tag="ACHKUB_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO6655235.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQAECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            complement(8488..8865)
                     /locus_tag="ACHKUB_002224"
     CDS             complement(8488..8865)
                     /locus_tag="ACHKUB_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6655236.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(8870..11566)
                     /locus_tag="ACHKUB_002225"
     CDS             complement(8870..11566)
                     /locus_tag="ACHKUB_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO6655237.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPISDPENGVWWLDGLPHATVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            complement(11704..12096)
                     /locus_tag="ACHKUB_002226"
     CDS             complement(11704..12096)
                     /locus_tag="ACHKUB_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO6655238.1"
                     /translation="MTVMVAVFILPGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEEL"
     gene            complement(12132..13619)
                     /locus_tag="ACHKUB_002227"
     CDS             complement(12132..13619)
                     /locus_tag="ACHKUB_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO6655239.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            complement(13609..14517)
                     /locus_tag="ACHKUB_002228"
     CDS             complement(13609..14517)
                     /locus_tag="ACHKUB_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO6655240.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARYAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            complement(14517..15179)
                     /locus_tag="ACHKUB_002229"
     CDS             complement(14517..15179)
                     /locus_tag="ACHKUB_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO6655241.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILENDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            complement(15176..15538)
                     /locus_tag="ACHKUB_002230"
     CDS             complement(15176..15538)
                     /locus_tag="ACHKUB_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO6655242.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGVIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            complement(15551..15865)
                     /locus_tag="ACHKUB_002231"
     CDS             complement(15551..15865)
                     /locus_tag="ACHKUB_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO6655243.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            complement(15942..16184)
                     /locus_tag="ACHKUB_002232"
     CDS             complement(15942..16184)
                     /locus_tag="ACHKUB_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO6655244.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            complement(16184..16525)
                     /locus_tag="ACHKUB_002233"
     CDS             complement(16184..16525)
                     /locus_tag="ACHKUB_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO6655245.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            16638..17204
                     /locus_tag="ACHKUB_002234"
     CDS             16638..17204
                     /locus_tag="ACHKUB_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655246.1"
                     /translation="MDEGKQPGTPGMNDIISIIILLTVYLIGLIFIILTINDIDRSSK
                     KRKYEKKLKEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFMTDNNFSHHVP
                     RVRELITWYEKYAPFTELPEDIKQQLQNIISLPDVSESEVQLLATSLHDIYNSCQKKE
                     KLARRINYVLGAVSIASFCISLFQILFK"
     gene            complement(17588..18349)
                     /locus_tag="ACHKUB_002235"
     CDS             complement(17588..18349)
                     /locus_tag="ACHKUB_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO6655247.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLYEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(18342..20438)
                     /gene="traD"
                     /locus_tag="ACHKUB_002236"
     CDS             complement(18342..20438)
                     /gene="traD"
                     /locus_tag="ACHKUB_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO6655248.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRQLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTERSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     NEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(20435..21223)
                     /locus_tag="ACHKUB_002237"
     CDS             complement(20435..21223)
                     /locus_tag="ACHKUB_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655249.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGERMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEAQLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            complement(21220..21702)
                     /locus_tag="ACHKUB_002238"
     CDS             complement(21220..21702)
                     /locus_tag="ACHKUB_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655250.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEEECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(21699..22292)
                     /locus_tag="ACHKUB_002239"
     CDS             complement(21699..22292)
                     /locus_tag="ACHKUB_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO6655251.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            complement(22289..22807)
                     /locus_tag="ACHKUB_002240"
     CDS             complement(22289..22807)
                     /locus_tag="ACHKUB_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO6655252.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVLSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMIVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(22804..23484)
                     /locus_tag="ACHKUB_002241"
     CDS             complement(22804..23484)
                     /locus_tag="ACHKUB_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO6655253.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEEQK"
     gene            complement(23463..24182)
                     /locus_tag="ACHKUB_002242"
     CDS             complement(23463..24182)
                     /locus_tag="ACHKUB_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO6655254.1"
                     /translation="MKRKILYLLVLCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPGILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(24186..24935)
                     /locus_tag="ACHKUB_002243"
     CDS             complement(24186..24935)
                     /locus_tag="ACHKUB_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655255.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAVHGEATFRVNGRQTVLRVE"
     gene            complement(24932..25531)
                     /locus_tag="ACHKUB_002244"
     CDS             complement(24932..25531)
                     /locus_tag="ACHKUB_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO6655256.1"
                     /translation="MKKRFILSGLTVCLLMAGCQGTQKLQQRDAAMPVSSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(25718..26686)
                     /locus_tag="ACHKUB_002245"
     CDS             complement(25718..26686)
                     /locus_tag="ACHKUB_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6655257.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRLEGIEKGRLEGIEK
                     GRQEEREKALQENYASARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            complement(26735..27295)
                     /locus_tag="ACHKUB_002246"
     CDS             complement(26735..27295)
                     /locus_tag="ACHKUB_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6655258.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIVGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(27311..27514)
                     /locus_tag="ACHKUB_002247"
     CDS             complement(27311..27514)
                     /locus_tag="ACHKUB_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655259.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(27605..28072)
                     /locus_tag="ACHKUB_002248"
     CDS             complement(27605..28072)
                     /locus_tag="ACHKUB_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO6655260.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT         6666 a         8740 c         6487 g         6488 t
ORIGIN      
        1 aaccgactaa cagacacaaa agaaatcctc cactcaaata gacacacatc ttattcacga
       61 taaacatagc ctttatactc ttcaatattt tcagttatat acaatttatt ttctgaatca
      121 tatttcaggt gggatgcata gagaacgcaa taccctgttt tatcagtgac agacaatcga
      181 aattcgtagg tctcgccctt ttccggtaaa aaagtataac ctacatcttc acagccatga
      241 agaaacttga aaccaatccg tgtcggagta ccagcataga catttgtatc ataatatgtc
      301 tctttataac gttcgggatc gttatttact tttggaaaac ctaaatcgtg tgttggtctt
      361 aaaatcacca tattagaagg atcaaccgca gcaggcttat ttccatactg gcttattgtg
      421 aaacctggtg gattaccgat taatctgaca tgcgctatgt tcttctctgt atctgacggt
      481 ggattatagg gaactttccc ccaataagaa gcacacccag atatcatggt cacaagagat
      541 atactaaaaa gattaattac tactcgattg gcgtttttca tagcaacatc catcaaacat
      601 catctttatt ttatgaaaat tattccacac ttatattcta tgtataacat acattgctaa
      661 atgtatttga tcaccatcaa aaaaacaaat gtaatatgct ggcgaattta tattactgag
      721 tttttcatat acgtcatcga cggttcatac gaatgatcgc agtctgatga tggtcgtgtt
      781 ttattttgtt aaacaagggt tatcaccaga caagtaccgg aggctggcac tgaagtacca
      841 actcgtgtga ttgcgttgct cgtgaaggac tgacactgaa atctcatgtc tagtgatgaa
      901 attctgacca agataaagcg cgcaatatga gacgtcttgt gatagttgcc cagtctggca
      961 actatccccc ttaatgggac gcttactcat aatctaacga taagaaacat tctcatctgg
     1021 atcatttcaa cagaatcccc gagccccagg gtaatgtggt atcgcagcct ctcatcaaat
     1081 ggaagacatg tgattgcaca tttatcatac aagacgtatg ataacactgt gttatttatc
     1141 ctgcgccgag ggtatagata tgaataacga atctgaaaaa gcaatgtggc gttgtaagcc
     1201 agtggataat ccttggacat tcagttcctt tgcaaaaaag gtcatttgtt cagtattacc
     1261 cgatactcag ccctcgttag acgcatttgt aaattcaaaa aactctgagg aacacttaaa
     1321 cttttcatca gactcaacct tcccttcatg cccagacatc aataacatca ataacatcaa
     1381 taacatcaat aattattatt gattctaagt ttttgtcgcc gaaaccaata atagtaaatg
     1441 gcggtaaaac cgccatttac gttgttattt tgatttcaca ccacttctcg caatagttcc
     1501 tgctgtctgc gctgctcccg aaacagtctc tgtaccacgt tgaattccag aagcaattac
     1561 ccctgcatgt accccagccc aggacaacgc acccagccac actgccggca agacgagaaa
     1621 catcgtcccc atcaccagat tcattatcag atcatccgat gtgttctgga atcccatcat
     1681 attgaaccgg gtatgtgtat ccgaactgta cagcgccgtc agcatccagc tgtcaagcca
     1741 ccgtgcaagc tcccaccaga acgtcagaaa gtgtaccgca aacaccacaa acgtcagtgt
     1801 gattaacgtc gacagttcat atgccgcaaa cgccagaatc agcggtatca tgatgtaaat
     1861 cgccatcagt attatcgact gtaccatcgg cagtgcctgc cgcattgcat ccagtgccgg
     1921 aaacgcaccc agtgctgcta ccgcactgcc cgccgttccc gcaatccggg ccaccgcatt
     1981 catggtcgtc atatctgcat tgccaccata gccacgatac acctctcccc cctgagagag
     2041 cgtcaggttt gccggactca ccagacgctt gatcacctcc tccttataat ccacctgact
     2101 gctgccaatc atcttcattg cagctgacag acgtaaccag taatggtcac tgacctcctc
     2161 catgacccgt tttttcagcc ccacactact gtcactccac cactcccggc atgtcggata
     2221 gcctccccgc cccgtatccg gtcgtccgct gtcacgtgct gtattccacg ggaacgcagc
     2281 ccgcggcatc tttgactgca gcgtcccgta atccccctgc atgaacgtgg cgctccctat
     2341 ccaggagata tcatcaagca cttttttatc tttactctgc ccctgatcac gctgtttcca
     2401 catatacagt gccagtgaat aacagtcgtt cgtaaagtcc tgcagctcat cagcaagtgc
     2461 cctgttttct ataaacgttc gctggatctc aaaacgcaac tggcgcaaat caggccggca
     2521 cggaatcgaa gccactgccg cctgggtgac acctttcgac aatttatgaa ccaccaccca
     2581 ccataccggg gctgcagcag tctgatcccc tatactgctg atcaccccac tgtaccccgt
     2641 ctctccgggc tcttcaggtg tccatatccc acaggtctgt gccctggatt tatcgaatga
     2701 cagcgtatcc agcgtcacct gtaccagagg aacacagcag gatatcatca caaaaaaagc
     2761 accatagaga acatgctcaa tacgcggcag ggaaagcatc cctttgtttc cctcatccgc
     2821 accttcttcc ctgactttca gccagatccc gatgatcctg aatgccagtg gcagggcaaa
     2881 cagtcctgtg ctgatcaaca cattccacag accgttattc accacccagc ccagcagagt
     2941 cagaaaatat tccagatagt tatttgtggt catgattgct caacgccccg gctcagcaaa
     3001 aatggcgtat tcacacagca ggataaacag aacactgact gtggccatac gcagaaaaga
     3061 agcccggtat tctgccctga accccggcgc atgccagatc ctccacccca gccatccgat
     3121 cgcactgtaa agacacagac gccatcccag ccagccataa cgtgtctcat acatccactc
     3181 ccgcagggcc gcaaatcctt ctgcgtgacg cataccggta tcggccagcc agagggaaaa
     3241 cacggtaatc cctgtcagca acagcgtata cagggcgaat accttcatcc tctttttcac
     3301 ccggtcataa attctgctca tcctctcact ccttatccgg ggcatcgaga gaatggatac
     3361 ggctgtcagc attgtcagcc tgctggatca tcgggttatt ccccacacgc tcgttatccc
     3421 gttcaatgat ggtcagaaca ctgttacgcg ccagatcccg cttcagctcc atctcgtttt
     3481 tcagcgccat aatttcccga tccagcgcct caatacggcg ttccccctct tccagtgcag
     3541 caggctgagc tgcagcatac ggctcggcca tccccgtgag cagcatcctg cgcatcagca
     3601 gcgccgtttc aacggtatcc gccatggcca gttctcccgc cagacgcgac gtcagggcac
     3661 cattgtccgg atcacgctgc agggcacgga tcactcccgc tgtcacagac agactcccgg
     3721 ctttcagttt tgccagattc ccggctgtcg gcttgtcacg accattgacc atacctgcca
     3781 gcagttccgt gttaacccgc gtcgcttctt caagcatcgg cgcaaacccc gtgccggcaa
     3841 tactggtccc cggctgctga tcagcatccc cgctggtaca tgcctttgca tcaccacagg
     3901 tccgcatgga tttatccccc agaaccttca ccaccgcccg ggcagcttcc tctgcattac
     3961 ggtatttcat gcaggcactc ccgttacagt tcccgggact gacactggat gtactggtcg
     4021 aaggcagact gttcatcata ttgaaaccgg cagctgtcag atcatgtgtc ggccggatag
     4081 cagactgtcc cgctccacca cgctgctgcc ctccgatcca gcgttgcccc tcctccccgg
     4141 taacctgacg acctgcttca tcccctttga tcgcatcacc gtcccccgta ttcacaattt
     4201 ttttgtactc ctcgagcatg gccgcctgcg tccacttatt gctgtccgtg aactccatca
     4261 tgcgtttcgc catgttctgg caattgagct gcgctttgtc gaatgccaca ttcgcctgca
     4321 gtacaccgtt ggtcagcatg tcatacagcc ccggattagc ccgctgtatc accattgccg
     4381 gcagactcgc cacggctccg ctcgccccct gaatcacatc ccccatcagg tttttgaaac
     4441 catcagtgat cccgttcagc tggttaccca ctgttgtttt caggtcaaaa ttgccacaca
     4501 tcagatcact gctccacccg agatccaccc caagtttctg catattgctg cgcgttgccg
     4561 gctgcgatat caccgagccc cctcccaggg tataaaacag tttgtctgat actgcgccgt
     4621 ccacggattt accgtagcca aacgcactgt cattcacttt cggcagggac aaaaagtttc
     4681 cgtcagcctg taccgtaccc gcacaaagca ccacagccag aacaggtatc gtcattttct
     4741 gtttcatcac ttccccttac ttaaaaattc gtactgccca ggaatgtctg tccccgtttc
     4801 ttgcagcaac tgtagggctg ccacagggca aaggcagcat tacctgtttc agccgcttca
     4861 tgctctccgt cagggaagac tgcgcaggac gtggagactg aaggcgtcag tctctgccat
     4921 ttatggttcc gggtgcccgt attttcctcc accggatccg gtggccagta accgtccttc
     4981 ttccctgctt tcaccggctg atagacatgt ggctcaccgg tgcgggtgat aatgtcagcc
     5041 acccgctggg ccaccaccgc agcagcttta tcatcatctg tctgggtcac gaacccggag
     5101 cggggataca cattgcccca catattctct tcttcctggc tgcctatttc gcgtttaccg
     5161 gggatcaggg cctccgggta cagtgactcc ggtagtcctg tacgccaggc aatactgtca
     5221 agcgtactga gaaaataagg aacaaaaggt gtcaccggtg actcacagga atatcccggc
     5281 accatccctc cgataagcgt ggtggccggg tgccctgttg catcggcata tttaaaacgg
     5341 attgacgaac gacgttcccc ctgtaccttc atgtctgcgg ggttccctcc tcctgctgac
     5401 agaccggcaa tacctttgct tatggcattc tccggaccac cggctgtact gctgaccgcc
     5461 gacatttcac gccagggatt tccgccaggt gcggtatagg tggaaacaac cacttccggg
     5521 atataatggg aaactttcac tgatgttctg accgtgcaac tgaatttggt gcagaacagc
     5581 cagtagcaaa tcccgctgac ccgccagctg atacagctct gtgaggctgc actggccact
     5641 atctgcgcgg tgttcagcga tgcctctgcc accggcaata ccgccatact taacagcaga
     5701 acaccggcgc gatgacgtga tgtgcaggaa atcatggctg ccctccccct gtacgcagtt
     5761 gttctgccag caccacgtct gtggttccgt agaccacttc actgtcatca aaaaccacag
     5821 ccggatattt tttcagtcca agggaccagg catgtgctaa tgcacggtag tgttcagcaa
     5881 gccctgcctg aaacgactgc cattcaggcg actgcatccg ttccatgaca cgggcagatg
     5941 cttctgcagg ctctgcggga agtaccccga aaaatgtatc ctgcgtctgc tcaggagcat
     6001 caagcaggac aacacgaaca cctgctggcg agttcatggg gcgctggtgc gaatcggtat
     6061 aaattgttgt accggcagat gctgccaccg gtagtatcag gaaggaatac agtaacagtc
     6121 ggtacatgat gcgactccgt cagacaactg gtatctgaca gcgtgcgcaa aggcaaaggg
     6181 acaatcagca ataaattcaa aaccggatga taaaaattaa gcctcttcct ctgaataacg
     6241 cacactctgc atctgaatca aattaatccg gttgcatcac aggaagccac tgattcgggg
     6301 taataccata ataacgcctg aacacagaaa taaaataaga agcactggca tagccacaca
     6361 actctgccac ccgggaaata cggttttctc ccgcagacaa aagctgagcg gcatactgca
     6421 ttctctcttt cagaagaagc tcactgaagg tcgtcagctc ttcctgaagc tttttcttca
     6481 gtaaactctc acttatatat aacagactgg ccacttcacc cagcatccat ggacgggaca
     6541 aatcagcact gataatattc ttcacccgga cagtgcagct ccggatacac cccgtgagca
     6601 gggcgtggaa atccggatgt tcctcaaaca gcgccagact ggaaaaagcc accgcatccc
     6661 tgaagtacac cggcgatggt tcatctgtaa taagttgtct gatcagacat tccgccagca
     6721 cggacatctg tggccggatc tgtaaaagca aagacgacgg gctgactgcc ataccggata
     6781 atggcgtgca gaaccgcaga taccgcctca ccacctcctc actgaggtca gcgataagga
     6841 aacgtccggt atgagcctga aaagcagggc gcagatgccc ggcaatcaga agcattgtgc
     6901 cggcaggtat gacagagttc ctgtgctcca gacaaagcat tgtctgtttg agagccataa
     6961 tgacagcgca ggtacagggc atcggatatc cggtaaatat gttgatttat taaagagaca
     7021 gacatgcccc gtatataaaa ttccgtgtca ccgggtgctg aaaaaaactg caccgacggc
     7081 gctcttttac tcctccgtta cggatatttc agcccccttt tcaggagcgg tatccttcac
     7141 atcactgaga ccggactgag tcgcttcaga gacctctgcc acctttccgg ctgcctgtgt
     7201 ttttacttcc tccacggcat ccttcacctc gctgagcccg gactgagccg cttcagagac
     7261 ctctgccacc tttccggctg cctgtgtttt cacttcctcc acggcatcct tcacctcgct
     7321 gagcccggac tgagccgctt cagaaacctc tgccaccttt ccggcagcct gcattttcac
     7381 ttcctccgcg gcatccttca catcactgag ttgagtctgc gcagcttcct ttatatctgc
     7441 cgccttctta cttacattct cctttgtcgc atcatcacag cccgcaacca gaaatggtgc
     7501 ggaaatacat aacgccagaa ttgttttttt cagcatacaa tctctcatta ttaagtgtta
     7561 acgcagaaca gcttatattt cgtccccgga aaatggtcaa aagacaacca gaacatcaca
     7621 gtgtcagggc acactcccgg catatgcttt tataaccata acatgctgtt cctgatatta
     7681 ccggaaatgt gcccactaca tttaacctgt tttcagcacc gggtcatcgg cggtgctgta
     7741 tttttttatt cccgtcacag aaacacacta ttaccgttga tttcatgacg aataaaatga
     7801 ataaattctc ccgtggtctt taccaccatt attataccaa taacaccatt tacacagata
     7861 cactccggga ctatctgttt ttgccccctc cgactccgcc ggaggttttt tattttcata
     7921 ctcaggcctt caccacttca caccggaagt cctccaccct gacgccaata taccgtcccg
     7981 gctcttttgc gggcgcaaac agtttttcac attcagcctg agtcattttt tcatcctggt
     8041 aacgcagcca ctgaaccggc atattgcctg gtttgattat tgtcgcagtt attttatatc
     8101 gctttcccat actggctctc atctcaacaa caaaatcacc ggctgagcca tcactccggt
     8161 aatgcatgcg aaaaaaagtg agcgtctcag caatggctca tgcggtgatt ctgtacggta
     8221 cccgcacaaa atacgggcac caacagcgtc gcagaataca acgaaggcat cagggcagca
     8281 cctctctccc gctatttttc agagttcttt accctcctcc tgacacggga gtacaaccct
     8341 gacagcatcc gggccagtac attcgcaagc acccatgaaa acacagccac acccacagct
     8401 cccgccagaa acagcacagg ttcactggca atttgatctg acagcgatgg atacagtggt
     8461 cccggaaaat cgatatcgct cctcatatca cccctctccc agccacaaca gcagatagaa
     8521 caaatccgcc gcatcagtca ctgatatata ccagcgggga tgaatatact cctcctccga
     8581 cggccagctc tcatgcccac tcacagtaag cagaatatgc acatactgac cctgctgacc
     8641 ttccagcatc accgccacac tgctgttcag acccaccctc tgtggctggc aacggataca
     8701 ctccacccgc cccagttgcg ccgccagatg cccgcgaaga cgttcaagat tatcccgtgt
     8761 ggtgcaactt atccccggcg caccctgtgg cggcagaaca aacacacttc cggcggggtg
     8821 aacagcagac accacttccg gttccggata cttcagacac agcatagtct cactccctgc
     8881 cagccagacc acgtagcccg tccagctttc gtgccacatg aaacgctgct tccagttcgc
     8941 tgcactggtg caccttcatc agagcacgtc gttcagcctt ctcttctttc tccgtcatcc
     9001 ccagagccag atacagactc ggtggcacaa cccggaacaa cgcttctgtg cgcttcgcca
     9061 gtaccacccc ctccgtatag cagccggaca gtttactggc tgacagcagt accgccttct
     9121 gctcctccgt cagggattta aaacgggcaa tattgttcac ttcctccggc ggcatggtca
     9181 ggcacaccca ccactctgcc atattcagca tcttctccga gacatccgga taatccttca
     9241 ggttctgcgt ggccagccac agccaggccc ccagcttgcg ccacattttc accactttgg
     9301 tcatatacgg ggacaaaagc gggtttaccg tcacgatatg cgcctcatcc accacaaaca
     9361 gaatatcccg tcccagatac tgatcgcgct cggcaatgtt gttaatcata ttggtcattg
     9421 acaccatggt cagtgccatc tgggcttcat aaccttcacg ggcaagatgc cccagatcaa
     9481 tcagggtcac atccgcttcc ggccacagtt ttccttcccg gttaaacagc tccgcttcaa
     9541 aactgcccgg ctgggtgaac atccccagcg actctgccat ctcagcagct ttagcccggc
     9601 gaacatcatt acgctgagtg tcccgcgaaa tttcccacaa cgcactctgc agatccaccg
     9661 gcagcatctg acgcccctcc cggtacgtgg tgtgcgtggc catcagcagt gcctcacgga
     9721 tcatcgccct gtccgcacgt ttgagcgctg cttcttcttt cggatcccct ccggtgatca
     9781 tcatgcgggc agagatttcc atctccccca gaatgtcgcg tttctcttcc tcttcatccc
     9841 cctcatcttc atcaagatcg ggcagtgaat gctcatccac actctgcagc gtctctccct
     9901 gctccaccag ttgatgagca tccgcaaatg gaggaagaca cacccccgtt cccggtttga
     9961 cactgatttt gttaaccgaa agcccgagac tctcaaaata atccgccagc agaccaaacg
    10021 agttcccggc ctcagcgata aacagacggg gacgatgaac tgccattatc tgcgacaacg
    10081 aaccacacag cgtggccgat ttaccggctc cggtaggccc gaaaagcagc agatgtgcat
    10141 tctgggtccg gtccagctta ttcagcggat caaaggtcag cacatccccg ccacggttaa
    10201 agaaactgaa tccgggatgc cccgtacctg tttcccttcc cgtcaccggc aggagccctg
    10261 ccagatgctg tacccacatc agacgcgtgt accactgttt tttatccgta tcaggatcaa
    10321 aacacatggg cagcgcgcgc agccagctgt tcagcggtcc gacatcaaac tccgggcgca
    10381 ctggctgtaa gcccgcgccc agcagcaccg tcgtcagttc aacccgtctg cgcttcagac
    10441 tctccatatc ctctgcccgc agcagaaacg tgatcccggc acggtagagc ttgtgccggt
    10501 ttcccagata tttcttcacg gtcgccacat cctcccgggc gcgcagggat tcactgttct
    10561 ccccgaccgc atttttcgac aacctcgtga agcgctcttc cagtgtgtcc tgtggctgga
    10621 ccacaattgt catacacagc accgttccct ccggaaaggt gtccatcagg gcattcactg
    10681 tcttttcgcc ccgggcctgt tcccctgtga tggttcccgg ctccggtggt gtgcgcagtt
    10741 tctccaccac aacagtggca tgcggaagcc cgtccagcca ccacacgccg ttttccggat
    10801 cagatatcgg tggggtgaac cagaggcttt ctgcaaaatc ggtcatcacc ggcatcattc
    10861 cctccggtgt ctcctgcggt tctgcacggg atgccatacg gtacagaata tccctgtcca
    10921 cccattccgg ccggggattg aacagcctca gcagccagcc atgcacctgc tgcccgttca
    10981 tccgtgtaca gcggacgcct gcgccaccca gcgcccccac gacccgcgaa cagacctggt
    11041 tcagcattgc caccggcggc atcggatcgt ggttgttttt cccgatccac cggtaaatca
    11101 ccatcctcgt gcgccgctgc tgaccacgcc atggctgacc tgtgaccagc gtatccctga
    11161 acagaccttc cggtcgggca atccccttca gatggcgctc catttccctc agccaggcct
    11221 ccgtaaatga ggagccttct gcatgaggtt tcacgtaccc cctgagctgg tcaacatacg
    11281 tgtccacgtc gttttcatcc tgacagaaga actgcaccac ccagggatgt gagtcatatt
    11341 catcaaaact gtcctgcagc gcatcctcca ccgtatcccg gatttgctcc agacgctctg
    11401 cggtccgccc ttccgtagcg gccggactca cctcatacac ggcaccaacc gacaccccat
    11461 catccagcag caggcactgc tcttcgtcca gatattccac ccatggcaga aaatcaatca
    11521 ccgacggaca ggcatggtaa accttcgtct catcctgtcg ggtcatcttc cccggccttg
    11581 acagtggctt tcgtacctga ggggccactc tttctttcat atccggagat accgcttctc
    11641 cgtcatgctg cgacaccggg gaagaaaccc gtttttttgt gcctgtaaac agggaaaatc
    11701 gcatcacaac tcctccgttc gttcacccgg catggcatac tgcgtccggc tgtaaaaagg
    11761 aaatactgtg ctgtaccccg gaaccggtga cgtaccggaa gccagatgcg gaaaaacata
    11821 catcaccatg tcaggattgg gcagccgcgg aaactgctga ctgatctcac tttcctgtgt
    11881 ccggctgtag cttatggctc cgtgcgcatc tgcctgctgc tccggcattg tcagcggacg
    11941 ccgcagggtg tccctggccg ctgtcatgtg ccgcgaatgt cctccgcttc ctttcccctg
    12001 ccagatctcc agcatactct gttcacccgg aggcagaagc tcctctttcg atgtcgagca
    12061 gccgggcaga ataaataccg ccaccatgac ggtcattaca gataactgtc gtttcacggg
    12121 atctccttac atcagtccag accaccggta ccgtggactt cccccggcag agtgaagtcg
    12181 taacgtaccc ggcgcccctg atcttcataa tcaatggcca gttgacgcgt gatatgcacc
    12241 gccagcgtct gccccggggg aacataaatg gcatcaaacg tctgcccgta tcgctgcttc
    12301 acccattccg cggtttcatt catcccgccg gacagggctt ttccaagtgc cgcctctccg
    12361 gcactgccgg tcagtgttga tgtgatgcca ttgacgttgt tctgtgaggt gtactgcccc
    12421 tcactcagtg catcacccgc agcgccggct gcagacaggg caaatattgt cggcagatac
    12481 gtggaggcat tggatttacg ggttccgctg atacacggga tcccgtcatc atccgaaatc
    12541 cagccaatgc ctcctgtact gctgttattt tttgcctgtg cgttactgct gccgttcacg
    12601 tctgatgacg gcagcgtacg cactgtcccg tcggcaaaaa caaacgtgat gctgttcacc
    12661 tgcccacgga cacaggagag cgtccagtca ccactcgcag taccggacac cactgcacct
    12721 tccacatccg gcagctctat tccgtttgcc gtcaggttat ccttacccac caggatcttg
    12781 aacggatagg gatccgtcac ggttccgttc accggaaccc gtcccagaag cgctgtcatc
    12841 gcccggctgc ctgccagcgt cgagttctcc ggcagggtat acactgcccg ggtgctcgcc
    12901 ttctgctgtt cattgctgta tcccttcacc tgctgttcat aagccgcttt ctgacgggtc
    12961 agtggattgt catccagaaa agagactgga aaacgcggtc cgctcttatc ccccaccgac
    13021 gacgcttcat cctgcacctg atcggccggt gtcacccaca tcagaccatc cgttcccggt
    13081 gaggctgccg atcctgtcac gctccccagt ccgtcaaggc ccagacctgc tggaatatcg
    13141 ctctcctgtc ctccggcgcc ggcatcagca ctctcctgct gacgcttcag ttgctctgtc
    13201 agtgactgaa cctgtgtcat taacgcgttc tgttcattcc ggaactggcg ctgcgactgc
    13261 tcataatttt tctgcaatcc tgccactgcc tcactgacct gaccggaaac atccgtgccc
    13321 cgcttgcgca gcatttcatt ttctttcagc aacttttcat tctgtttgtc cagtaccagt
    13381 tgccgctccc tcacctgatt catacgccca atcagtgtgc gcagggtatc ttcaggtgtg
    13441 tctccttcca cgcccagggc cttcagttca tccggtgaca gtccggccag cgttcctgag
    13501 ctttccgcag acgagggctc cccggtatct cctccacagc tttttaatcc cgtcaggatc
    13561 cccgccacca ccacacaggg caccacatac ttcacgatcg tattggattt aatctgcatg
    13621 cttcacctcc ccccgtgaag gaatgcgacg ggaggcaggc tccgcgataa acgcctgttc
    13681 cggccgttct gccgtcacca catagagcgt ggtggtatcc tcaggggtac ctgctgcacc
    13741 cagccagcgg tgctggaaag tcgcggcaat gaattttccc tgaagtgccc gcggatcaag
    13801 gataattttg cggcttaccg tactcgtcag tttcagtgca acaactgcac gcccacccgc
    13861 agcccaggct gccagtggcg tgactgtcag tggctctgac ggatagagtg tggtgatttt
    13921 cccgggcaga tgtgtattaa ccggccggat acccggtacc gcttccagcg tccgtaacgg
    13981 tgcgtacagc gactgtgccg catagcgggt caacagaacg ggtaaaggct ctgcatactt
    14041 caccggctga cggagggcct gcgagccgtc atccccgccg ggtaccacct ttttgtgttc
    14101 tgcatagcgt gcagacgccg ctgtgccagc ctgactgacc gtactgacgc ttccctcata
    14161 cacaatccgt acgggctctg tgggcccttt ttctgctgct gtcacatcca gcagaataat
    14221 ttctccgctc tccacatcct gcagttgcag acgcgtctgt ggaaaaacac tctctgcttt
    14281 cagatagact gccccaccgg tactctgtac cctcaacttt ccgttcagtg ctggcgggaa
    14341 gcccacccgc acatttttat ctgcaaaaat aacccgctcc tgtcctgcca tcagggtaac
    14401 gggtaaggga atgcgctccc atttcatcag ctcatcagcc cgtgcgctca gtggtaaaac
    14461 cagcagtgac aggaccatca caatactacg gtataaacaa cgttcagctc cgttcatcac
    14521 tgattatccc ccttcccggc tgccggggag gctgcttcca gccgctgtgg tgtactgctg
    14581 tagcagtcca gcgccagccc gaacggatta cgttcaggat ccccctccat gcgcaccacc
    14641 tttatcggat aacggacaag tgcccgtttt accggctcag catgaaaata ctcatctgcc
    14701 accagatcca gctgtacctg ccagttatcc cgcccttcca ccgtcaccgg atttttgttg
    14761 tacctgcccc cgtagccatg ttccggaatt tcatacacca cgcgcacacg ctccagcagc
    14821 tctcccgcat cactgcgacg gcgggcatca ttctccagaa tctcccgaca ggatggcgtc
    14881 aggtaggcgg agagcgcatg aattttcgcc gggtagtcca cctctccgtt cttcggccag
    14941 gcattcagtt gctgaaagat ataaaaggca aaggaataaa ccgttgacgg cgggacttcc
    15001 caccagggac gggtactgcc ggaacgcaga tccggcggat tgtgaattgt cagtctgtcc
    15061 ggtgccgtca tccacccgat acagaccagc atcagaattc cggccagcac accgctgaaa
    15121 atacgcagtg tcaggatatg ctggtcgcga tcccgcagag catgacgaaa acgactcatt
    15181 ttttccccct gctacgggta cggcgcaggg accagccatg cgcctcccgg ataaatgttt
    15241 tccggccacc accccatgca ctgcttttct tcgccagatg ctgccagagc cagttgtcag
    15301 gttttccccg cttcagccgg ctgatccaga cgccaccgaa ccagaccagc agcaggggag
    15361 tcaacaacat ccctgtcggg ataatcaccc agccaagtag aggaatcagc ggtaacggcc
    15421 agatcacccc tgccacagcg cctgtcaggg ccgccagccc cagttccggt gtggtaaaac
    15481 cacggaaaac cgcaggctcc acgttcagac gatcgggcag aaaacgaatg gtctgcatgg
    15541 ggatgtctcc ttacagaatg atggaggccg acttgcccag cagccagata atggcgacca
    15601 gcagaacaac accaaccacc acgatggcac caaactgccc ccatccggcc ttcccgtcac
    15661 gaacctctga gaaggtggaa agcgccgctt ccgccacctt cacaaaagca actgcactga
    15721 gcacaagacc aaacagcaca acaccatcct ggagatagcc tttgatctgt ccgaaaagtc
    15781 caccacctcc accggatgtt ggcggctcta tctgaggcaa atcagccaga gcaggtgtga
    15841 tcccggtaca cagaaacacc gccatggcat accaggcctg gcgacagatg cgcatttttt
    15901 tatgcattat ttcctcctga aacttaaaca agacactgaa atcaacccgc aaacatccag
    15961 atactgcaga tcagcagcag gacgagtcgg aacataaacc agccgaacac accatcccgg
    16021 acatcaccgc tacgccagcc tgcccaggca tccgtcagac tccacgctgc ccagaggaac
    16081 acgactgcca gcagaaatcc cagcgtgatg agatgcagca cactgagatc cacttcacct
    16141 gaagcagccc ggaaggccgt attctgcagg gccgtcatcg ccatcaggga cgctcccgcc
    16201 ggtagtcacc ggtcacggct gcggaagacc ggggttgcgc ccgggaaggg gccagataat
    16261 ggtcaatacc gtcacgaatg gtctgcagat ctgcacgggc acgcaaataa tcaaaaaagt
    16321 agcgttcccc ctgtgaagaa gcctgcagag aggccgttct ggcccgctcc agcgctgcct
    16381 gcacgtggtc aagctggcgc attacagtgg ccagttcatc cttttcggca gcctgcgcag
    16441 gtaatgcaaa ggacaaaaga aacagcatcc ccgcggcacc ggccagacgg ccggcacggc
    16501 aggatgatgc cggaaaagaa aacatacgca cctccgtcag agttaaacag tgcgtacagg
    16561 atgcgtaagg caggggactg gatcagcgat aaactctatt tgcaaaacag aaaattttta
    16621 cgggagaata cactggtgtg gatgaaggca aacaacccgg aactcctggc atgaacgaca
    16681 tcatcagtat aatcattctc ctgacagtct atctcatcgg cctgatattc attatcctga
    16741 ctatcaatga tatagacaga tcatcaaaaa agagaaagta cgagaaaaaa ctgaaagaag
    16801 cgataataaa tggcagttta caaaataatg atatctgttt actggccgaa cgctggtctg
    16861 ccagtcatga acatatcaaa agcgcactgc tgtctgtttt caatgatttt atgactgata
    16921 acaacttttc acatcacgtc ccccgggtca gagaactgat cacatggtac gagaaatatg
    16981 ccccattcac agaactccct gaggatataa aacaacagtt acaaaatata atcagtttac
    17041 ccgatgtcag tgagtcagaa gtgcagttat tagccacgtc acttcatgac atttataatt
    17101 cctgtcagaa aaaagagaag ctggccagaa gaataaacta tgttctgggg gctgtcagca
    17161 tagccagttt ctgcatctcg ctgttccaga ttctgttcaa ataaatttat tccagcctga
    17221 atgatttatt cctgtttatt aaaacacggc atcactactg tttcccgtta atggcaccgc
    17281 attaacaatt taaagaggaa acacatcagc atggaatttc tccgtactgc ccttccgttc
    17341 tgggccctgt tccttctgac ctgggtttgt gttgtgtatt ttttcattta tgaagaacct
    17401 cgttgccccc cgtggatcta tgacgtcatc agggccatca ctctgttcat tattttcgtc
    17461 aacattgttg tcattccggc gctgtcgtta ctctgacact gacgcgcact gaccgtaagg
    17521 gcgtggcttg tctgccgaca ccctgccaca aaacaagttt tgcatcaggg tgtccggcct
    17581 ctgtggatta taaatatttc ttgaaggatg ccatggtcac ggtgatggcc acccccagca
    17641 gcatggccgc cggccatatc agcaggttcg gatacaccga cgtcggccag gacaggtaaa
    17701 gaatgcaggg aatatatatc gccggtttaa tagacttttt tgcatggtga taaacaaagc
    17761 tggactcata cgcagcacca taccggcgca gatcacgacg ccccagtccc tccacaaccg
    17821 cgatcagtat caccatcaca aacagcggaa cagacaacac caggatcgtc acccgtatca
    17881 gtgtgaccac cgtgacatat actgtggcca tcaggtactc atacagataa tggctcagcc
    17941 agcgccccac atcctcactg tactgtgaca cactgttacc caccacggca ccggcatttt
    18001 gctgcttcag ccacgacaga aaacctgttt ccacaaacag ccactgatac gccgttgcta
    18061 tccattctgt caccgttacc gatggtgtcg acagcagcag actgcgggtg aattccgaag
    18121 acagccaccc cagttctgtc ttcatcactg cctcactgtg tgctgccccc tgctccggcc
    18181 agaaaaaagc aatcccgata tactcaacca gcaggctcag cagcagggag gaaaacagga
    18241 tcccgaccag catccacggc cagccccaca acagattcca gagcagtccg tgctgacgtg
    18301 gcggagaggg ttgtgctgct ctgttcacct cacgacgggg ttcagccatt ctgctctcct
    18361 tccggcatgt tcacggaggg agtctcattc caccatccct ctccggtgtg gtaattacgc
    18421 cgcatggcct ctgcaatttt tgcaatactc tccggcatca gcgcgtcatc tgcatcagtt
    18481 gcaggcaggg gcatacggat tttccacaac tgtccccctt ccagcagagc aaaggcctgt
    18541 cctttcggca gggtcacgat atctgccggc tccagcaaag gcaccttcac agtaccaaca
    18601 cgatcctggg tactggatgt gaagtcctgg ccggtcgtga catctgcggt atcctgatga
    18661 ccggaaacca gcgttttggt ataaatttcc acctgggaca actgggtcgt cagtaactcg
    18721 gccgtccggt tctccctgac ccgcagcata atcatgttat taaagttacc ctgcacctgg
    18781 gccgttttgg cggcactacc ggtacgggcc tcaatatcag aagaggtctg cgtgtaagcc
    18841 gtcacctgca taccggcacc gcctcccttg ttgattaacg gaatgaactc atcgcccatc
    18901 agttcattaa actcatcaca atgcaggtta accggatttt tggtaccatt ccctcccgga
    18961 agaccggcat tcattccgtg tttgtagata tgcccggcga cactcaccag atccgcaaac
    19021 atggagttac ccacggcact ggccacctca ctgtcactca gcgcatcaag accaatgtac
    19081 accaccgcct ttttacgtat cacctgctcc cagtcaaaca cgggacgggt atcgctcata
    19141 tccaggtagt caggtgccag cagttctgcc gtctttcctg tggtcagttt ttccagcaac
    19201 ggcaacaggg atgccacaat tttgtcaaaa taggtacgat cataacgtac cgcgcttcgc
    19261 agaccatcca gtatcggatc atagagtttc tttccggctt cactgctgag agccacctca
    19321 atggcccaga tacgcacagc atccggctgg ttctgcatgg tccggggaac atccgcttcc
    19381 cccagtacat tcatgttatt cagaatggcg ttttccagtt ccggcatctg ttcgttgatc
    19441 accttaccag cataacggat ataaaggtcg gcaatattat tcacataacg cataatcagc
    19501 gtgtaatccg ggcgctcccc cagtgccacc agtgcacggg caacaatgtt gacaaaccgc
    19561 catgcaaact cacggaaggc cgcactgtta ccttcaccgg ataactgacc ggccacacgg
    19621 gaagccacct ccgacacacg accaaaacgc cccacggcgt tataacgcgc agagatttcc
    19681 ggccagccca gatgaaagat ataaagctca tcgcccctgc cggcacggtg tgcttcagcc
    19741 cagacacgca gcaacagatc cgcatcccct ttgggatcaa agacaatggt gacatctccc
    19801 cgacgaatat cctgtgtgac cagcaactcc gccagccggg ttttcccgac acgggtggtg
    19861 ccatagacaa tggtgtgccc cacacgttca cccagtgcca gcgtcacatc ctgctcatcc
    19921 ggctcaatac catgcagagc aggatttcct cccaccggcg gcaggggacg gacgggattc
    19981 agcggcgagt cacgacgaag aagctgccca atccagggaa gactgcgctc cgttttcatt
    20041 tcaagccagc gcgctgtccg gtacagactg cccggctgaa gatagtgcgc aacctccggt
    20101 cgcagcgtgt cctgaaggcg ctgggtgtgc ttctgtgtcc agcggaagcc gcgtcccaga
    20161 aacagccgct gacggctgac cggaatttgc cgggaactca tcacataacg gggcagctgg
    20221 cgcagattgc gacggtaacg gatgatcttc atcccctgtc ggaaacggac cactgccaga
    20281 acgccaaagc cggcggctgt gacatagctg accgaaggcg cgagcgccac cgcccagggc
    20341 gctgtcacac agacaccggc cgccgttgct gccaccacgg cggtattcag ttccacagcc
    20401 ggacgcagca gagcctcaat cacataacgg ttactcatga ccgggtacct ctctgtaaaa
    20461 gacgccgcca ccatgagcgc ggcaacgctt tctccggtga cgctgacagt gcctccagcc
    20521 tgcgctgtaa caccatcacc tcccgccatg cgtctgccgc attcagcagt aatgccttat
    20581 acaccagtgg cccgtcatgt ccgccggcat gcacatcatc cactgtcaac tgcgcctctg
    20641 ttctgaaacg atccactttc cagcaggcca ggtcttcgcg ggaaacacac tgccgccgga
    20701 tctgcagacg aagatacaca tcatgccggg acaacggttc tgccaggttg tgaatttccc
    20761 gacaggcaga ccggaacgcc tggtctgtgt gataaatact ctgggccaca ttgtgaagct
    20821 gtgtgcgccc ctccccctgc aggtggaaca gaaagcgggc cagacagctg cggtgattac
    20881 cgtcagcact gacataccag tgaatgccat ccgtgctgac aaaacccatc ccggcatgct
    20941 gttgctctgc ctgcgtgtaa tatcccggat tcttttcaag taacgggata ttgatgtcca
    21001 tgcgttcacc acgctccagc agctccagcc agctcattcc ggcatactgt ggatggtcag
    21061 tccccacaat gcggtacaca ttgatactgc cctgtcccgt ccagtagtgt tccgtaataa
    21121 aatccgacat tgccgcaaac tctgcggtat cccatgcaac cagttgttca tgtgcccagg
    21181 ccggagcatt cgcctgtatc cgctctgtaa atgccccctt cattcagctc ttctctcctg
    21241 cacttttgag ataccgatac cgtcaggcca cggcataaca gccgggcccc ccctgccttc
    21301 cccggatacg gctgcagaac cacaaccacg aaacagaatg actgacgaga caccttccat
    21361 aaacaaagcg cccagcaaca gaaccactgc ctgcattttc agttcacgaa tatcctcagc
    21421 aacagcctcc ccgaaagaaa tcgctgttcg cggacattcc tcctcatcga catacatacc
    21481 tgagacggat atgacggagg aacatttttg taccatcaca tgcccttcag gcgcatcacg
    21541 ccagcgggat gactccgtcg cataacgctg tgtcatgcct gcctcaccac cactcagatt
    21601 gttccagaca tacagcatta ccagacatcc tgccagcatt gacggccaga caatcagaag
    21661 ccggagcagc agcataacaa accggtacag tgtcataatc atgatcttcc tttattactc
    21721 agtaaggtgc cattcagcag ttgcaacaga cgttgcccac tgatcagctc aatatccggc
    21781 cacagtgtca gccgtgcccg gctgatgttc cccgtacgcc ccgtatggat aaaaaagccc
    21841 ctgcatccct tccggcgcag cagttcgcca aaagcatcaa tatgtgccgg acgaatggtt
    21901 cttccgtaac gtttagcctg tatcagccag cgttcaccgt taatcaccac ctgaccatca
    21961 agaccaccat cgccgctgta tgaggggtta cggataaccg gcatcccctg ttgctcaaaa
    22021 gccagcagca acagttcctc aaacacatac ggactgattt tgcgcagata tttcagccgc
    22081 cgggcatcac cctgcaattc gttcagccgc ttcattaccc gtgaggccgt ggcacgataa
    22141 cgctgatgcc ggcgtgttct cacacacgct ttttttcgcc ggctgcgcca cagtgccggc
    22201 cccaggacaa cagccagcag gctgagaacc aggcaggctg tcagtaccgg agaagcagag
    22261 agcgcagcag aagtggcaga cagcggcatc atggtgcagg tacctgtgac aggccgttgt
    22321 ccgttatcag caccgggtaa tgacttatct gtaacctgcg ggccagtaca gaaccggatg
    22381 ccggaaccat caccaccccc ggtgccagct cacgcagtac gcgcagactg ctctcatccg
    22441 ccacattcac tatcattccc gccgccttcc gctccctgag tactgctgca ttcatctgca
    22501 gccatgtgcg ggaatgggca tcatcgccca caagaaaaag tgcaccgatg cccggtaact
    22561 gaagccggcg cgccacaacc tctccgggtg acatttcagg cgtactgacc ggtaacattg
    22621 ccgcctctcc ctgcggaaaa gagggtgata gcacaactga agcgggaacc ggtggctgcc
    22681 ggtttatcgc ctcaaaaaag gggctggcat ccctgccgcc caggtctgca atcactttca
    22741 gttctgcgcc agctgttgta cagaacagca gcagactcag tacataaaac tgtctttctc
    22801 gtttcatttc tgctcctcct gtggttcagt ccagatataa cggggggatg gtgtcagcat
    22861 ggcggtcgct gccacatgca catcagacgg attcaggaaa ttatcccggt ggagccgggc
    22921 aagatgccgt ctgaccacgg cggtataacg tgcggccggc gcaccacctg ccgggtggtg
    22981 ataacacccg gccacctgca gccagctgac cggatggcga cgccggcatt cctgcagaat
    23041 gaccgctgcc gtattcagac tggtgtaagg atcaaatgcc gcccatgtgg acgggaaacg
    23101 atgtccattc cagcccagat tgacctgtgc tatcccgaca tcaatacgtt ttgccggata
    23161 atgtttcaga aacacctgca atgcctgcca ggcctccaga cgtgtggcat aacggtaccc
    23221 ttttcctgcc acatttattg tccagggcca gggtcgggcc acgtccggct gacgaaaatc
    23281 acgacgccgt tgtttcagta ctccggacat cgcttccgga gacatggacg tttccgtcag
    23341 ggaaacggca tacaaaagct ctgcgggaat accatgggca cgggccaccc gcacgtaccc
    23401 gtccggtacg ttctgaacgg cagcacgaca gacaccactg caggccacca tcagcagaac
    23461 cgtcagaatg ccgccacgcg ccatccgtca gccccctgct gcagtaccac cggcattttt
    23521 ccttcaccaa aacgcagcca gcgtccggca tcatgattca gggtgatatg acggctgcga
    23581 actttctcca caggtatgct gtgcgcatgc gcccactgac gcagaatctc atcctttccc
    23641 tgactgtcga caaggtagat atcaacctgc cgtcccgtgg ccagtacgga ggcaagacgg
    23701 gcatcacagg tgccacattc cttcgccttc acaaacagcg ccagccgtcc ccccgtatct
    23761 ccggtaagtg ctcctgtttt tcccatactg acaggcaaga taccgggata caggcgcttc
    23821 caggccgcat tcacctcctg ctgaaacgcc agctccttct ccgttcgttc atattcagcc
    23881 ctgacccact tttcggccca gacacgtcgc tctgccggcg ttctggcctc tataccgaga
    23941 accgtcagcg gatcaagtcc gggagactgt atcccccggg ggccggacat cagtgactga
    24001 taacgccggt aatcctcctc actcacaccc cactgcccgg cctgctgctg aagtccggat
    24061 gtggtactct gcgtcaccac cgtaccggtc gtccgttgcg cctctgacga catattcgtc
    24121 tgcccggcgg actgagccag aacggggcac agtaccagca gatacagtat ttttcgcttc
    24181 atcatctact ccaccctgag caccgtctga cgaccattca cccggaacgt tgcttcacca
    24241 tggacggcat acacaagcgt ccacccggcc acactctctc cttcacccag cagagccacc
    24301 tgagagaggc tggtataccc ccggggagcc acggctgcac gggtactgcc accgcgcagc
    24361 tctgtcccgg tcagcacaaa aggagcctgc cgaactgtat gaactgcgcg ggatttacgg
    24421 gaggacgtca ccggagcagg ctttttctcc tccgtttttt tcatccccgc tttcctgctg
    24481 tccggctgtg acggtgactc cggtactttc tgcatcttct ccaccagacc gctgagtgta
    24541 tccagtctca gggaaagctt ttcccgggca gactcaatct gctccagcat ctccggcaga
    24601 ttctccagtt gttccttcca ggatgctgtc agcacgggct gctgctccag gactgccagg
    24661 cgcttttcca tatccttcag ttgcttctcc gtctctgtca gtttcattct caggggcgcg
    24721 agttctgacg cagaaagcat ttctcttctc tggccttcca gggtcgtgat ccggctgtca
    24781 agaccggtca taagcatcag tacgccatat accactgccc ccatgaccag ccccacagtg
    24841 acagacagta tcaacacgcc ggggaaacga ctttttcttc gctccggcga cataccagac
    24901 gcaccctccg gtatctgcgg ctgtttctca ctcatttttt cagaaaccct cctgttgcct
    24961 tcacacccgg ttcacgaccc acaaaaggaa cggtgaccgg aacaggagcc agctggctga
    25021 ccggtaactg gtaaccgtca cgcagactgt ggcagaccac cctctgcaca tcatccactt
    25081 ccagttgcca tgccggcccc gccattaact gcagggcagt acgcagtctc agtggtcctg
    25141 gctgatactg caccgccggc agaggctgac ggtacagcac gccgttggcc ggtccggtgg
    25201 cgcagagcgt gtaaccggac tgtttcaggg cataacgcat ggcatcggcc accgtgggtt
    25261 tcagggagga gggaatgcga atgtcgatca cctgagaaag tggatcacgc tgtacagcct
    25321 gcggctctgt gctgaccagc aggtaacggt catagcgcac cacctccggt gcctgagaat
    25381 acacatccgg gctggcaggc tgtacattac ggtttactgt gaccgacgat accggcatgg
    25441 ccgcatcccg ttgctgtagt ttctgagtgc cctgacatcc cgccatcagg aggcagactg
    25501 tcagtccgga caggataaag cgttttttca tcgggtattt acctctgcag tgataaatca
    25561 gaggtcaatg tgcagaagaa gacggggctg gctcaatgaa caactcatta cccactggcg
    25621 aaaaaaaaac gcctgcagac atgcaggcgc tttgttgaca gaaaggaaag caacataaca
    25681 aatatgtcac aatcctgcgg ttaacataaa ttaccgatta ccggagagca ttacgcaaat
    25741 catcatccga gaactgcagt atttgtttga ttttctctga cgatataccc atctgctgca
    25801 tgcgacgggc cgatgcgtaa ttctcctgaa gagccttttc tctctcctcc tggcggccct
    25861 tctcgatacc ttccaggcgg cccttctcga taccttccag gcggcccttc tcgataccag
    25921 ccagttccag tcgttcagcg atagtcatta gatcatcctc atgtcttggc agacgttctg
    25981 ccatcgcccg tataaattca ggtgaagctg aatcacattc tttcaccata tagttaaaca
    26041 atgtcaccac ctgagtgtgt gtttcctgta tctgaatcaa ctgtgacaaa aagtcaagat
    26101 gaaccatcag atccactttc ttctgccgga gcttcatcat aagcatcagc gcagccatct
    26161 gcttatagcg acggataccc tcatcatcaa gaagaccgac atcaatcacc ctgggtggcc
    26221 tgccttctcc ataaagctcc cgaccaaacg ccggatcatc caggcagtca ggccagttca
    26281 tgctgtatgg ataaggactg ggtacaccat ggtaaaaaag caccggtatc acaacgggag
    26341 cacacgcatg tgctttcagg tggcgatgta tcaccgccag aacatagcgc atcattcgca
    26401 gggccatata cttatccgcc ttgctctggt gctctgtcag cgtataaatg tatccatccc
    26461 tgccatctgt ggttttcata ctccacagaa catcacaggc aaactggcga agatccggat
    26521 ccacaaacgt ggtcggctcc agttgcaggg tgttcatatc gcaccggttc agttgttccg
    26581 ggggcaacgc gcctttcagg aaatccctcg caacagaggg acattccatc atcgctctga
    26641 acgccgcatc atgtggtgtg ggggtagtgg gtgtgttttc attcacaacc gcctccttga
    26701 attaaaagtg gggcggtaag ccccgtggtt aacatcagat gacatggata atccgtcgtt
    26761 caacgccata cccacgcccc ataaagggga tatcatcgtc aaagtccatc ggcggcattc
    26821 ccccgcttcc tgaggaagcc tgaccgtttt ttcccgttga ttgttgtgca ttttgtggtg
    26881 gttgtcccca tcctccctga gcaggagcag atgaaacatt tccaccaccc tgctgctgac
    26941 gcggagaccc cagcatctgc agggtaccat tgacactcac aacaacttcc gtggaataac
    27001 gatcaacacc attctgctga tcgaaccact tccgggttct taactgacct tcaatataca
    27061 cctgtgaacc tttacgaagg tattcaccga caatctcagc cagttttccg taaatcacaa
    27121 cacggtgcca ttcagtgtgt tccttcattt caccgctctg tttatcacgc caggtatcag
    27181 tggtggccag cgtcagattt gccactgcac ctccgccgtt ggggaaataa cggatttccg
    27241 gatcctgtcc cagatgacca atcagtatga ctttattcac tccacgcgat gccataacaa
    27301 ctccttatcc tcacacacat tcacgctgag tccagatgcc gttttccaga gcccggactg
    27361 cctgctcata atttctgaaa tactcttcgg attcccggga cacaggccct tcgtcgcact
    27421 gagtaccaat ataataacca cgagcactgc tgaggatctg cagaggtaag gtatagcccg
    27481 ctttggcggc cagaattccg cttgtcggtt tcataacatg tttccttaaa gttaaacgat
    27541 aagaaaacag acctccctga tggttggtct gttttcatca gggaggttga gttgaaaaat
    27601 acgatcagaa agaattttct gcgtattccc gcggggtatt gccattctgt ggcggtatgg
    27661 attccgattt ttcagtctta taaaccatgt cctggcctat tttgatccac tccaccttga
    27721 tcaggcgggc tttcagactg acgcgttgct caccggcatg ttcaccccgg ttaagggtaa
    27781 aaatgtcagt agaggggttg ctcatggtaa aaccaatcag caccttgcga tcttcatcca
    27841 ccgctttctg gcagcgggca atcagggaag ttgcctcttt accggcaacc gtcacatcaa
    27901 aacgcacata ggacgggctg tccgtcggac cactcagtgc attcacaaca caactgagaa
    27961 atgtcccgtt ctgatgatta acctgacgaa tgttgctgag ataccccagg cctttcagtg
    28021 tcagattgaa gtactcagtt ttagcgtttg cagaagaagt atttgcagtc ataatgatat
    28081 ctccattctc agtatcggaa agacagagaa catcatcatc cctgacgggt tgtgatattc
    28141 ccgtcaggga gccggaacag aactattccg gctgactcag taacagttaa agaaaacctt
    28201 catcgtcatg actgacgata tctgctctca aaggtgggca gatttaccac caccagaaac
    28261 atcctggctg cacatcccct ttcaggttac gggggtgttg atcacccgaa ggcgttcctc
    28321 tcagatcaac ggaacattgg tgcccaccga agcgggatgt ggtcacccga aggcgctcct
    28381 c
//
LOCUS       DAZFRN010000054        28160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFRN010000054 DAZFRN010000000
VERSION     DAZFRN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.054"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHKUB_002249"
     CDS             complement(<1..209)
                     /locus_tag="ACHKUB_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6655261.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKUB_002250"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKUB_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO6655262.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKUB_002251"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKUB_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO6655263.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKUB_002252"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKUB_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO6655264.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKUB_002253"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKUB_002253"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO6655265.1"
                     /translation="MLHTLHRSPWLTDFAALRRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKUB_002254"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKUB_002254"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO6655266.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKUB_002255"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKUB_002255"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO6655267.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTYDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKUB_002256"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKUB_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6655268.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKUB_002257"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKUB_002257"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6655269.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKUB_002258"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKUB_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO6655270.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKUB_002259"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKUB_002259"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6655271.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKUB_002260"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKUB_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO6655272.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKUB_002261"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKUB_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO6655273.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKUB_002262"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKUB_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO6655274.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKUB_002263"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKUB_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6655275.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKUB_002264"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKUB_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6655276.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKUB_002265"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKUB_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO6655277.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKUB_002266"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKUB_002266"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO6655278.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKUB_002267"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKUB_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO6655279.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHKUB_002268"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHKUB_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO6655280.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKUB_002269"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKUB_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO6655281.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKUB_002270"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKUB_002270"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO6655282.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKUB_002271"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKUB_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO6655283.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTARSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKUB_002272"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKUB_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO6655284.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKUB_002273"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKUB_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO6655285.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKUB_002274"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKUB_002274"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO6655286.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKUB_002275"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKUB_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO6655287.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKUB_002276"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKUB_002276"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO6655288.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKUB_002277"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKUB_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO6655289.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKUB_002278"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKUB_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO6655290.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKUB_002279"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKUB_002279"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO6655291.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6807 a         7556 c         7121 g         6676 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 accacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gccgcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcataggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcatttact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacgt ccaacagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gagtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 acacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaggaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcaac cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggttcgt tacaggtgat cacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 cgtatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acaccttccc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctagac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagct tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgatggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatagaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcacgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcgtgtgaa atcgccattc agcgttgtca gcagcgactg gaatatgacg aaccggggag
    17581 ttctggcgat gccaatatca tggattcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac cactgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gttgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 tgactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcataatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tcctggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggagaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccacgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggct tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagtcc
    19921 gcttcccaca cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg gcgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gagctggaag ttgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtatcg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggttac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgata acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattatc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gcgacgagca ggtcgcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25561 acatcgatcc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccaag
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cttgattttc acgctcggta atttgctcga
    26041 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26161 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaatct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggcggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gctatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcgt ttattgcatc
    26461 cggttatgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27061 ccgctcaccg ctcatggtag cggtctcctc tggcggcacc tctccggttc tggcacgcct
    27121 gttgcgcgaa aaacttgaat cactgctgcc gttacatctg ggccaggtag cgaaatacgc
    27181 cgggcaatta cgcggtcgag tgaaacaaca gttcgccacg atgggtgagc gtcgccgttt
    27241 ctgggagaaa ttgtttgtta acgaccgcct ggcgcagtcg ctggccaaca acgatcagaa
    27301 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gactgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggacc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 ccaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa actggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacatag ccccttaata
//
LOCUS       DAZFRN010000055        27767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFRN010000055 DAZFRN010000000
VERSION     DAZFRN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.055"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..190
                     /locus_tag="ACHKUB_002280"
     CDS             <1..190
                     /locus_tag="ACHKUB_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO6655292.1"
                     /translation="VKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGERKIT
                     NKCLHNSCIIKICPYGLK"
     gene            complement(293..1543)
                     /gene="icd"
                     /locus_tag="ACHKUB_002281"
     CDS             complement(293..1543)
                     /gene="icd"
                     /locus_tag="ACHKUB_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO6655293.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1715..2368
                     /gene="rluE"
                     /locus_tag="ACHKUB_002282"
     CDS             1715..2368
                     /gene="rluE"
                     /locus_tag="ACHKUB_002282"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO6655294.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2378..2839
                     /gene="nudJ"
                     /locus_tag="ACHKUB_002283"
     CDS             2378..2839
                     /gene="nudJ"
                     /locus_tag="ACHKUB_002283"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO6655295.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2893..3999
                     /gene="mnmA"
                     /locus_tag="ACHKUB_002284"
                     /gene_synonym="trmU"
     CDS             2893..3999
                     /gene="mnmA"
                     /locus_tag="ACHKUB_002284"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO6655296.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4035..4676
                     /gene="hflD"
                     /locus_tag="ACHKUB_002285"
     CDS             4035..4676
                     /gene="hflD"
                     /locus_tag="ACHKUB_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO6655297.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4680..6050
                     /gene="purB"
                     /locus_tag="ACHKUB_002286"
     CDS             4680..6050
                     /gene="purB"
                     /locus_tag="ACHKUB_002286"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO6655298.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6218..6889
                     /gene="phoP"
                     /locus_tag="ACHKUB_002287"
     CDS             6218..6889
                     /gene="phoP"
                     /locus_tag="ACHKUB_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO6655299.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6889..8349
                     /gene="phoQ"
                     /locus_tag="ACHKUB_002288"
     CDS             6889..8349
                     /gene="phoQ"
                     /locus_tag="ACHKUB_002288"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO6655300.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8425..9546
                     /gene="roxA"
                     /locus_tag="ACHKUB_002289"
                     /gene_synonym="ycfD"
     CDS             8425..9546
                     /gene="roxA"
                     /locus_tag="ACHKUB_002289"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO6655301.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9692..10921)
                     /gene="pepT"
                     /locus_tag="ACHKUB_002290"
     CDS             complement(9692..10921)
                     /gene="pepT"
                     /locus_tag="ACHKUB_002290"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO6655302.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11171..12307
                     /gene="potA"
                     /locus_tag="ACHKUB_002291"
     CDS             11171..12307
                     /gene="potA"
                     /locus_tag="ACHKUB_002291"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO6655303.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12291..13148
                     /gene="potB"
                     /locus_tag="ACHKUB_002292"
     CDS             12291..13148
                     /gene="potB"
                     /locus_tag="ACHKUB_002292"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO6655304.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13145..13939
                     /gene="potC"
                     /locus_tag="ACHKUB_002293"
     CDS             13145..13939
                     /gene="potC"
                     /locus_tag="ACHKUB_002293"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO6655305.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13936..14982
                     /gene="potD"
                     /locus_tag="ACHKUB_002294"
     CDS             13936..14982
                     /gene="potD"
                     /locus_tag="ACHKUB_002294"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO6655306.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMQNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            15040..15501
                     /locus_tag="ACHKUB_002295"
     CDS             15040..15501
                     /locus_tag="ACHKUB_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO6655307.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            15498..16286
                     /locus_tag="ACHKUB_002296"
     CDS             15498..16286
                     /locus_tag="ACHKUB_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6655308.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGCQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16425..17246)
                     /gene="cobB"
                     /locus_tag="ACHKUB_002297"
     CDS             complement(16425..17246)
                     /gene="cobB"
                     /locus_tag="ACHKUB_002297"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO6655309.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17262..18173)
                     /gene="nagK"
                     /locus_tag="ACHKUB_002298"
     CDS             complement(17262..18173)
                     /gene="nagK"
                     /locus_tag="ACHKUB_002298"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO6655310.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(18202..19446)
                     /gene="lolE"
                     /locus_tag="ACHKUB_002299"
     CDS             complement(18202..19446)
                     /gene="lolE"
                     /locus_tag="ACHKUB_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO6655311.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19446..20147)
                     /gene="lolD"
                     /locus_tag="ACHKUB_002300"
     CDS             complement(19446..20147)
                     /gene="lolD"
                     /locus_tag="ACHKUB_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO6655312.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(20140..21339)
                     /gene="lolC"
                     /locus_tag="ACHKUB_002301"
     CDS             complement(20140..21339)
                     /gene="lolC"
                     /locus_tag="ACHKUB_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO6655313.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21601..22674
                     /locus_tag="ACHKUB_002302"
     CDS             21601..22674
                     /locus_tag="ACHKUB_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO6655314.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22802..26248
                     /gene="mfd"
                     /locus_tag="ACHKUB_002303"
     CDS             22802..26248
                     /gene="mfd"
                     /locus_tag="ACHKUB_002303"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO6655315.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGATEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKSGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26392..27354
                     /gene="ldtC"
                     /locus_tag="ACHKUB_002304"
     CDS             26392..27354
                     /gene="ldtC"
                     /locus_tag="ACHKUB_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO6655316.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27437..27694)
                     /gene="bhsA"
                     /locus_tag="ACHKUB_002305"
                     /gene_synonym="ycfR"
     CDS             complement(27437..27694)
                     /gene="bhsA"
                     /locus_tag="ACHKUB_002305"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO6655317.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6806 a         7166 c         7000 g         6795 t
ORIGIN      
        1 ggttaaatct ggaattatcc ttaaagataa agaatatcct attactattt ataacatcgc
       61 accattcaac aaggatttat ggagtctact caaaagcagt caagagtgtc ctccaggaga
      121 aagaaaaata acaaataaat gtttgcataa tagttgcatt ataaaaatat gcccatatgg
      181 gctcaagtaa agggttttat ataaatatgc tcgatatgct attaataata cctacataaa
      241 taaaacaacg ggcgtgttat acgcccgttg caatattaaa cacatgtagt gattacatgt
      301 tcttgatgat cgcatcacca aattctgaac atttcagcag tttagcgcct tccatcagac
      361 gctcgaagtc ataggttacg gtcttcgcat tgattgcgcc ttccatacct ttaacaatca
      421 ggtctgcggc ttcagtccag cccatatggc gtaacatcat ctcagcggag agaataatag
      481 agccagggtt tactttgtcc tgaccggcgt atttcggcgc agtaccgtgg gtggcttcaa
      541 acagggcgca ttcgtcaccg atgtttgctc caggggcgat accgataccg ccaacctgcg
      601 ctgccagagc gtcagaaatg tagtcaccgt tcaggttcat acaggcgata acatcatatt
      661 cagccggacg cagcaggatc tgttgcagga atgcatcagc aatcacgtct ttaatgacga
      721 tctctttgcc ggtgttcggg tttttaactt tcagccacgg gccgccgtcg atcagttcac
      781 cgccaaactc ttcacgcgcc agctggtagc cccagtcttt aaacgcgcct tcggtgaact
      841 tcatgatgtt gcctttgtgc accagagtca cagagtcacg atcgttagca attgcgtatt
      901 cgatcgctgc acgaaccaga cgtttggtgc cttcttccga acacggctta ataccgatac
      961 cacaatgttc cgggaagcga attttcttca cccccatctc ttcacgcagg aatttaatca
     1021 ctttctcggc gtcggcagag tctgctttcc attcgatacc cgcataaatg tcttccgagt
     1081 tttcacggaa gataaccata tcggtcagtt cagggtgttt aaccgggctt ggagtgcctt
     1141 gatagtaacg taccggacgc aggcagatgt agagatccag ttcctggcgc agggcaacgt
     1201 tcagagagcg aataccgcca ccaacaggag tggtcagcgg acctttaatg gcaacgcgat
     1261 attcacgaat cagatcaaga gtttcagcag gcagccagac gtcctgacca taaacctgtg
     1321 tggatttttc accggtgtaa atttccatcc aggagatttt acgctcgcct ttataggctt
     1381 tctcgactgc agcgtcgacc actttcagca tggctggggt tacatctaca ccgattccat
     1441 caccttcaat gtaagggata atcggatttt caggaacgtt gagtttgccg ttttgcaggg
     1501 tgatcttctt gccttgtgcc ggaacaacta ctttactttc cattcacctc tccttcgagc
     1561 gctactggtt tgctcgtctg ccaccacgtt gcatatgcgt ttgcgtcctg cgatacggat
     1621 gctttagagc aattttttgt taatgatttg taattggctt gtcaatacta ccctattgtt
     1681 tgccgtcatg aaagatgcgc gttattaggc tataatgcgg caattcataa tctctgaaaa
     1741 taccatgcaa aaaacttctt ttagaaatca ccaggttaag cgattcagct cgcaacgttc
     1801 taccaggcgt aaacctgaaa accagcccac gcgtgtgatc ctgttcaata aaccctacga
     1861 tgttcttccg cagttcaccg atgaagccgg acgcaaaaca ttaaaagaat tcatcccggt
     1921 tcagggcgtt tatgcagcag gtcgtcttga ccgcgatagc gaagggttac tggtgctgac
     1981 caataacggc gcgttgcagg cgcgtttaac ccagccgggt aaacgcaccg ggaaaatcta
     2041 ttatgtgcag gtggaaggta ttcccacaca agacgcactt gaagccttgc gcaatggcgt
     2101 aaccttaaat gatggcccta ccctgcccgc cggcgcggag ctggttgacg aaccagcgtg
     2161 gttatggccg cggaatccac caattcgtga acgcaaaagt attcccacca gctggctgaa
     2221 gatcacctta tatgaaggac gtaatcgcca ggtgcgccgc atgaccgccc atgttggttt
     2281 ccccacgctg cgactgattc gctatgcgat gggagattac tctttggata atcttgccaa
     2341 tggcgaatgg cgagaagtga cagattaagg atgcaaaatg tttaaaccgc acgttaccgt
     2401 tgcttgcgtg gtgcacgcag aaggcaaatt tttagtcgtt gaagagacga ttaatggtaa
     2461 agcgttatgg aaccaacctg ccgggcatct ggaagccgat gaaaccttag tggaagccgc
     2521 cgcccgtgaa ctgtgggaag aaaccggcat cagcgcgcag ccgcaacact ttattcgtat
     2581 gcatcagtgg attgcgccag ataaaacgcc gtttttgcgt ttcctctttg ccattgagct
     2641 tgagcaaata tgcccgaccc agcctcatga cagcgatatc gactgctgtc gttgggtcag
     2701 cgccgaagag attttaaagg cgtcaaatct tcgttcgccg ctggtggcgg aaagtatccg
     2761 ttgttatcaa agcgggcaac gctatccgct ggagatgatt ggcgatttta actggccttt
     2821 tacaaagggt gtcatctaaa aggctgcgtg atagaatacg ccgccttgaa gttcaatgtc
     2881 gtgagtgatc caatgtctga aaccgcaaaa aaagtaatcg tcggcatgtc cggcggtgtc
     2941 gattcctccg tttctgcctg gctgttgcaa caacagggat atcaggttga aggcctgttt
     3001 atgaagaact gggaagaaga cgacggtgag gaatattgca cagcggcagc ggatctggct
     3061 gatgcccagg ctgtctgcga caagctcggc attgaactgc acaccgttaa ctttgctgcc
     3121 gagtactggg acaacgtctt cgaactgttc cttgccgaat ataaagccgg tcgcacgccg
     3181 aatccggata ttctgtgcaa caaagagatc aaatttaaag ccttcctcga atttgccgcc
     3241 gaagatttag gtgccgatta tatcgctacc ggtcattacg tacgtcgggc cgatgtcgat
     3301 ggcaagagcc gcctgctgcg tggtctggac agcaataaag accagagcta cttcctttat
     3361 acgctcagcc atgagcagat tgcgcaaagc ctgttcccgg tcggcgaact ggaaaaaccg
     3421 caggtgcgta agattgctga agatcttggt ctggtcaccg cgaagaaaaa agactctacc
     3481 ggcatttgct tcattggcga acgtaaattc cgcgagttcc tgggccgtta tctcccggcg
     3541 caaccgggca aaatcattac cgtcgatggc gatgaaattg gtgagcacca ggggctgatg
     3601 tatcacactc tcggtcagcg taaaggtctg ggtatcggtg gcaccaaaga aggtaccgaa
     3661 gaaccgtggt atgtggtgga caaagacgtc gaaaacaaca ttctggttgt cgctcagggc
     3721 catgaacacc cgcggctgat gtctgtcggg ttgattgccc agcagttgca ctgggtcgat
     3781 cgcgaaccat tcaccggcac tatgcgttgc acggtaaaaa cccgctatcg ccagaccgat
     3841 atcccttgca ccgtcaaggc gctggacgat gatcgcattg aagtgatttt cgatgaaccg
     3901 gttgccgccg tgacgccggg ccagtctgcc gtcttctata acggtgaagt gtgcctcggt
     3961 ggcggtatta ttgagcagcg tctgccgctg ccggtctgat tattatcttt acttaaaaca
     4021 aggaagcagt gaacgtggca aagaattact atgacatcac cctcgccctg gccggtattt
     4081 gtcagtcggc acgcctggtg caacaactcg ctcaccaggg gcattgtgat gccgatgcgc
     4141 tacacgtctc actcaacagt attattgata tgaaccccag ctcgacgctg gcggtttttg
     4201 gcggtagcga agccaacctg cgcgtcgggc tggaaaccct gctcggcgtg ctcaatgcca
     4261 gcagtcgcca gggcttaaac gccgaattaa cccgctacac actcagcttg atggtgcttg
     4321 agcgcaaact ctcctcagcg aaaggcgcgc tcgacactct gggcaaccgg atcaacggcc
     4381 tgcaacgcca gctcgaacac ttcgatttac agtccgaaac gctgatgagc gcgatggctg
     4441 ctatctatgt tgatgtgatc agcccgcttg gcccgcgcat tcaggtcacc ggttcccctg
     4501 ctgtactgca aagcccacaa gtgcaggcga aagttcgcgc aaccctgctg gcaggcattc
     4561 gcgccgccgt gctctggcac caggtcggcg gcggacgtct gcaactgatg ttttctcgta
     4621 atcgcctgac cactcaggca aaacaaattc ttgctcattt aaccccggag ttgtgatcta
     4681 tggaattatc ctcactgacc gccgtttccc ctgtcgatgg acgctacggc gataaagtca
     4741 gcgcgctgcg cgggattttc agcgaatatg gtttgctgaa attccgtgta caagttgaag
     4801 tacgttggct gcaaaaactg gccgcgcacg cagcgatcaa ggaagttcct gcttttgctg
     4861 ccgacgcaat cggttacctt gatgcaattg tcgccaattt cagcgaagaa gatgccgcac
     4921 gcatcaaaac catcgagcgt accactaacc acgacgttaa agcggttgag tatttcctga
     4981 aagaaaaagt ggcggagatc ccggaactgc acgcggtttc tgaattcatc cactttgcct
     5041 gtacttcgga agatatcaat aacctctccc acgcattaat gctgaaaacc gcgcgtgatg
     5101 aagtgatcct gccgtactgg cgtcaactga ttgatggcat taaagatctc gccgttcagt
     5161 accgcgatat cccgctgctg tctcgtaccc acggtcagcc agccacgccg tcaaccatcg
     5221 gtaaagagat ggcaaacgtc gcctaccgta tggagcgcca gtaccgccag cttaatcagg
     5281 tggagatcct cggcaaaatc aacggcgcgg tcggtaacta taacgcccat atcgccgctt
     5341 acccggaagt tgactggcat cagttcagcg aagagttcgt cacctcgctg ggtattcagt
     5401 ggaacccgta caccacccag atcgaaccgc acgactacat tgccgaattg tttgattgcg
     5461 ttgcgcgctt caacaccatt ctgatcgact ttgaccgtga cgtctggggt tatatcgccc
     5521 ttaaccactt caaacagaaa accattgctg gtgagattgg ttcttccacc atgccgcata
     5581 aagttaaccc gattgacttc gaaaactccg aagggaatct gggcctttcc aacgcggtat
     5641 tgcagcatct ggcaagcaaa ctgccggttt cccgctggca gcgtgacctg accgactcca
     5701 ccgtgctgcg taacctcggc gtgggtatcg gttatgcgct gattgcgtat caatccaccc
     5761 tgaaaggcgt gagcaaactg gaagtaaacc gtgaccatct gctggatgag ctggatcaca
     5821 actgggaagt gctggctgag ccaatccaga cagttatgcg tcgctatggc atcgaaaaac
     5881 cgtacgagaa gctgaaagag ctgactcgcg gtaagcgcgt tgacgccgaa ggcatgaagc
     5941 agtttatcga tggtctggcg ttgccagaag aagagaaagc ccgcctgaaa gcgatgacgc
     6001 cggctaacta tattggtcga gctatcacca tggttgatga gctgaaataa actcgtatca
     6061 gtgccggatg gcgatgctgt ccggcctgtt tattaaaatt atccactttt tcttttttca
     6121 ctttacctcc cctccccgct ggtttattta atgtttaccc ccataaacac ataatcgcgt
     6181 tacactattt taataattaa gacagggaga aataaaaatg cgcgtactgg ttgttgaaga
     6241 caatgcgttg ttacgtcacc accttaaagt tcagattcag gatgctggtc atcaggtcga
     6301 tgacgcagaa gatgccaaag aagccgatta ttatctcaat gaacatttac cggatattgc
     6361 gattgtcgat ctcggattgc ctgacgagga cggtctgtca ctgattcgcc gctggcgtag
     6421 caatgatgtt tcactgccga ttctggtatt aaccgcccgt gaaagctggc aggacaaagt
     6481 cgaagtatta agtgccggtg ctgatgatta cgtgactaaa ccgtttcata ttgaagaggt
     6541 gatggcgcga atgcaggcat taatgcggcg taatagcggc ttggcttcac aggtcatttc
     6601 gctacccccg tttcaggttg atctctctcg ccgtgaatta tctattaatg acgaagtgat
     6661 caaactgacc gcgttcgaat acaccattat ggaaacgttg atacgcaata atggcaaagt
     6721 ggtgagcaaa gattcgttaa tgctccaact ctatccggat gcggagctgc gggaaagcca
     6781 taccattgat gtactgatgg gacgtctgcg caaaaaaatt caggcacaat atccgcaaga
     6841 agtgattact accgttcgcg gccagggcta tttgttcgaa ttgcgctgat gaaaaaatta
     6901 ctgcgtcttt ttttcccgct ctcactgcgg gtacgttttc tgttggcaac ggctgcggta
     6961 gtactggtgc tttcgcttgc ctacggaatg gtcgcgctga tcggttatag cgtcagtttc
     7021 gataaaacta cgtttcggct gttacgtggc gagagcaatc tgttctatac ccttgcgaag
     7081 tgggaaaaca ataagttgca tgtcgagtta cccgaaaata tcgacaagca aagccccacc
     7141 atgacgctaa tttatgatga gaacgggcag cttttatggg cgcaacgtga cgtgccctgg
     7201 ctgatgaaga tgatccagcc tgactggctg aaatcgaatg gttttcatga aattgaagcg
     7261 gatgttaacg ataccagcct cttgctgagt ggagatcatt cgatacagca acagttgcag
     7321 gaagtgcggg aagatgatga cgacgcggag atgacccact cggtggcagt aaacgtctac
     7381 ccggcaacat cgcggatgcc aaaattaacc attgtggtgg tggataccat tccggtggag
     7441 ctaaaaagtt cctatatggt ctggagctgg tttatctatg tgctctcagc caatctgctg
     7501 ttagtgatcc cgctgctgtg ggtcgccgcc tggtggagtt tacgccccat cgaagccctg
     7561 gcaaaagaag tccgcgaact ggaagaacat aaccgcgaat tgctcaatcc agccacaacg
     7621 cgagaactga ccagtctggt acgaaacctg aaccgattgt taaaaagtga acgcgaacgt
     7681 tacgacaaat accgtacgac gctcaccgac ctgacccata gtctgaaaac gccactggcg
     7741 gtgctgcaaa gtacgctgcg ttctctgcgt agtgaaaaga tgagcgtcag tgatgctgag
     7801 ccggtaatgc tggagcaaat cagccgcatt tcacagcaaa ttggctacta cctgcatcgt
     7861 gccagtatgc gcggcgggac attgctcagc cgcgagctgc atccggtcgc cccactgctg
     7921 gacaatctca cctcagcgct gaacaaagtg tatcaacgca aaggggtcaa tatctctctc
     7981 gatatttcgc cagagatcag ctttgtcggt gagcagaacg attttgtcga ggtgatgggc
     8041 aacgtgctgg ataatgcctg taaatattgc ctcgagtttg tcgaaatttc tgcaaggcaa
     8101 accgacgagc atctctatat tgtggtcgag gatgatggcc ccggtattcc attaagcaag
     8161 cgagaggtca ttttcgaccg tggtcaacgg gttgatactt tacgccctgg gcaaggtgta
     8221 gggctggcgg tagcccgcga aatcaccgag caatatgagg gtaaaatcgt cgccggagag
     8281 agcatgctgg gcggtgcgcg gatggaggtg atttttggtc gccagcattc tgcgccgaaa
     8341 gatgaataaa tatgtccata cttcacgcat tacgttaagc atccgttata atcggttgca
     8401 gataccagcc tgtggatgct taacatggaa taccaactca ctcttaactg gcccgatttt
     8461 cttgaacgtc actggcagaa acgcccggtg gtgttaaaac gcggctttaa taattttatt
     8521 gacccgatct ctccagacga gttggcgggt ctggcgatgg aaagcgaagt tgacagtcga
     8581 ctggtcagtc accaggatgg caaatggcag gtcagccacg gcccgttcga aagctacgat
     8641 catctcggtg aaaccaactg gtcattactg gtacaggcag taaaccactg gcatgagccg
     8701 accgccgcgc tgatgcgacc gttccgtgaa ctaccggact ggcgtattga tgatctgatg
     8761 atctcttttt ctgtacctgg cggcggcgtc ggcccgcatc tcgatcagta cgacgtgttt
     8821 atcattcagg gtaccggacg tcgtcgctgg cgagtgggcg aaaagctgca aatgaaacag
     8881 cactgcccac atccggatct gttacaggtc gatccgttcg aagccatcat cgatgaagag
     8941 ctggagcctg gtgatattct ttatattccg ccaggattcc cgcatgaagg ctacgcgctg
     9001 gaaaatgcga tgaactattc cgtgggtttt cgcgcgccga atacgcggga actgattagc
     9061 ggatttgccg attatgtgct gcaacgtgaa ctgggcggca actactacag cgatccggat
     9121 gttccacctc gcgctcatcc tgcggatgtt ctgccgcaag agatggataa actgcgtgag
     9181 atgatgctcg aattgatcaa ccagccggaa cactttaagc aatggtttgg cgagtttata
     9241 tcccagtcac gtcatgaact ggatatcgcg ccgccggaac cgccttatca gccagatgaa
     9301 atctacgatg cgctgaaaca aggtgatgtg ctggtgcgcc tgggtggtct gcgcgtattg
     9361 cgcattggcg acgacgtgta tgccaatggt gagaagatcg attccccgca ccgtccggca
     9421 ctggatgcac tcgccagcaa cattgcgctg actgcggaga attttggcga tgcgctggaa
     9481 gatccgtcat tcctcgcaat gctcgcggcg ctggtaaata gcgggtattg gttcttcgaa
     9541 gggtaagttt ggatttaagc cggatgtggt gctgattgcg ccttatcgtg cctacatgca
     9601 ctgaacgcct gtaggcacga taagtatgtg gaccagtgtc aatataacgg tttcatcccg
     9661 gtaattggca gtccacatac gcatcgggca atcacgactt ccgttgcgcc gttaactcgg
     9721 caatacggac gatcacctgc accgcttttt ccataccttc cagagtcaca aactcatgct
     9781 taccatgata gttgtaaccg ccagtgaaca ggttcgggca aggtaatccc ataaacgaca
     9841 actgcgcgcc gtcggtaccg ccgcggatcg gtttcagttc cggttcaata tcgcagtcac
     9901 gcatcgcctg ctgggcgata tcgagaatat gcggatgctc aaccactttc tcgcgcatat
     9961 tgtagtaact gtcttcaatc accagttcaa tgtagcaatc aggatgtaac cctttgccca
    10021 cttttttggc gatctccatc attttacgtt tacgcgcttc aaactgttta cggtcgaaat
    10081 cacggatgat gtaatgcata tcggcccgtt caacggtgcc tttcatgctc gccaggtgat
    10141 agaaaccttc atagccttct gtcatttccg ggctttcatc cgccggaact tccgcatgaa
    10201 tacgtgccgc cagcgacagc gcatttacca tcactccttt cgccgtgccc gggtgaacat
    10261 tgttaccgac aattttgata ttgaccgacg cggcgttgaa gttttcaaac tccagttcgc
    10321 ctacgccacc accatcaaca gtgtaagccc agcgggcatc gaaggcgtca acatcaaaat
    10381 gtttcgcccc tttgcccact tcttcatccg gggtaaaggc gacgcgaata tcaccatgcg
    10441 gaatgttttt ctgttgcaat accgctagcg cggtcatgat ttctgcaata cctgctttgt
    10501 catcggcacc taacaaggtt ttaccatcgg tagtaatcag cgtctgaccc agtagctgat
    10561 gcagcaccgg gaacataacc ggtgataaaa cttcatcgcc gatacccagc gcaatatcgc
    10621 caccgcgata gttttcaaca atttgcggat tcacattttt gccgctgcaa tccggtgagg
    10681 tatccacatg agaaataaag ccaatcgccg ggatattgcc agggacgtta gccggtaacg
    10741 tcgccatcaa agtgcccttc tcacttaagg tcacattgat aagccccatc tcttcgagct
    10801 gctctttcag tagatgcaat aacttccatt ggccttccgt gctgggaacc tgtctcaccc
    10861 ctgcttttga ttgggtatcc agagacacgt agttcaaaaa tcgctcaagt agtttatcca
    10921 tgtagtcacc ctcacttttt gtgacaacat tattaagaag caagaaaaga caaatattgc
    10981 gtcaggtcac ttttacccct gcaagcggga atatttatca gcattacctt aatgaatata
    11041 aagctaagcc agtaattcac aacaaagatt ggcgattcaa gcggtttgcc gtagaattac
    11101 cgccctcatt aagagtcacc aaggtggtta accacaaacc ccgcatcggt aagccatccg
    11161 ttgcgtttac atgggacaga gtaaaaaatt gaataaacaa ccgagttcgc tttcaccgct
    11221 ggtgcaattg gcgggaattc gcaaatgctt tgatggtaaa gaggtcattc cccagctgga
    11281 tctgactatc aacaatggcg agttcctcac gctgcttggc ccttctggct gcggtaaaac
    11341 aaccgttctt cgcctgattg caggtctgga aactgttgat tccggacgca tcatgctgga
    11401 taacgaggac atcacccacg ttccggcgga aaaccgctat gtgaacactg ttttccaaag
    11461 ctacgcactt ttcccccaca tgaccgtgtt cgaaaatgtg gcctttgggt tgcgcatgca
    11521 aaaaaccccc gctgctgaaa ttacgccccg cgtgatggaa gccctgcgga tggtgcaact
    11581 ggaaaccttc gctcaacgta aaccgcatca gctctctggt ggtcaacagc aacgcgtcgc
    11641 cattgctcgc gcggtggtta acaagcctcg tctgttgttg ctggatgagt cgctctcagc
    11701 gctggattac aaactgcgta agcaaatgca gaacgagctg aaagcgttac agcgtaagct
    11761 tggtattacc ttcgtctttg tcactcacga tcaggaagaa gcgctcacca tgtcagacag
    11821 gattgtggtg atgcgcgagg gtcgtattga gcaagatggt acaccgcgcg aaatttacga
    11881 agagccgaaa aacctgtttg ttgccggctt cattggcgaa atcaatatgt ttaacgccac
    11941 tgtcatcgaa cgtctagacg agcagcgcgt acgcgccaac gttgaaggcc gcgaatgtaa
    12001 tatctacgtt aacttcgccg ttgaaccggg gcaaaaactg catgttctgc tgcgcccgga
    12061 agacttacgt gttgaagaga ttaacgacga caaccacgct gaagggctga ttggttacgt
    12121 tcgtgagcgt aactacaaag gcatgacgct ggagtcggtt gttgaactgg aaaatggcaa
    12181 gatggtgatg gtcagcgaat tcttcaatga agacgaccct gactttgacc actctctcga
    12241 ccaaaaaatg gccattaatt gggtagaaag ctgggaggtc gtactggctg atgaagaaca
    12301 caagtaagtt ccagaatgta gtgattgtca ctattgtcgg ttggcttgtg ttgtttgtct
    12361 ttctgcccaa cctgatgatc attggcacca gctttttgac ccgcgacgac gccagtttcg
    12421 tcaaaatggt ctttacgctg gataactaca cgcgtctgct cgatccgctc tattttgaag
    12481 tgctattgca ctcgctgaat atggcgctga tcgccaccct cgcctgcctg gtgctgggct
    12541 acccgtttgc ctggtttctg gcgaagctac cacacaaggt gcgtccgctg ctgctgtttc
    12601 tgctgattgt tcccttctgg accaactcac tgattcgcat ctacgggctg aaaattttcc
    12661 tcagcaccaa aggctatctc aacgagtttt tgctctggct gggcgttatc gacacaccaa
    12721 ttcgtatcat gttcacgccc agtgcggtga ttatcggtct ggtttacatt ctgctaccgt
    12781 ttatggtgat gccgctgtac tccagtatcg aaaaactgga taagccgttg cttgaggcgg
    12841 cgcgcgatct cggtgccagc aagttacaga cttttatccg tatcattatt ccactgacga
    12901 tgccgggaat tattgccgga tgtctgctgg tgatgctgcc agcgatgggc ctgttctatg
    12961 tatccgacct gatgggcggt gcgaaaaacc tgctgatcgg taacgtcatc aaggtccagt
    13021 tcctcaatat tcgtgactgg ccgtttggtg cagccaccag cattacgctg actatcgtaa
    13081 tgggcctgat gttgctggtt tactggcgcg cttctcgtct gctgaataaa aaggtggaac
    13141 tcgaatgatc ggtcgactgc ttcgcggcgg ttttatgacc gctatctacg cgtacctgta
    13201 tatcccaatc attattttga ttgtgaactc ctttaacagc tcgcgctttg gcatcaactg
    13261 gcagggtttt accaccaaat ggtatagcct gctgatgaac aacgacagcc tgttacaggc
    13321 agcgcagcat tcactaacaa tggcggtgtt ttcggcgaca tttgctacgc ttatcggttc
    13381 actgacggca gttgcgctgt accgttatcg ctttcgtggt aagccgttcg ttagcggaat
    13441 gctgtttgtg gtgatgatgt caccagatat cgtgatggcg atttctctgc tggtgctgtt
    13501 tatgctgctg ggtattcagc ttggcttctg gtcgctgctg ttctcgcata tcaccttctg
    13561 cctgccgttt gtggtggtga cggtgtattc gcgcctgaaa ggttttgacg tgcggatgct
    13621 ggaagcggcg aaagatctcg gtgccagtga atttaccatt ctgcgaaaaa tcattctgcc
    13681 actggcaatg cctgcggtgg cggcgggctg ggtgttaagc tttaccctgt cgatggacga
    13741 tgtggtagtt tcttcgttcg tcaccggacc aagttatgaa attctgccgt taaaaattta
    13801 ttcgatggtc aaagtcggcg tatcgccgga agttaacgcg ctggcaacca tattactggt
    13861 gctgtcgctg gtgatggtta ttgccagcca gcttattgct cgtgataaaa cgaaaggtaa
    13921 cacaggggac gttaaatgaa aaaatggtca cgccacctgc tcgcggcggg tgctctggca
    13981 ctgggcatga gcgccgctca cgccgatgac aacaacacgc tgtatttcta caactggacc
    14041 gagtacgtgc cgccaggact gcttgaacag ttcaccaaag aaaccggtat taaggttatc
    14101 tattcgactt acgagtcgaa cgaaaccatg tacgccaagc tgaaaacgta caaagacggt
    14161 gcctatgacc tggtggttcc ttcaacctat tacgtcgata aaatgcgtaa agaagggatg
    14221 atccagaaga tcgacaagtc gaagttaacg aacttcagca atctcgatcc agacatgctc
    14281 aacaagccgt ttgacccgaa taacgactac tccattccgt atatctgggg tgcgacggcg
    14341 attggcgtta acggtgatgc ggtggatcct aaatctgtca ccagttgggc agatctgtgg
    14401 aagccagagt acaaaggtag cctgctgctg accgacgatg cccgtgaagt gttccagatg
    14461 gcgctgcgta agctgggcta ctccggtaac accaccgatc cgaaagagat tgaagctgca
    14521 tataacgagc tgaaaaaact gatgcaaaac gtcgcagcgt ttaactccga taacccggct
    14581 aacccgtaca tggaaggcga agttaacctc ggcatgatct ggaacggttc tgctttcgtt
    14641 gcacgccagg cgggtacgcc tattgacgtg gtgtggccga aagaaggcgg catattctgg
    14701 atggacagcc tggcgatccc ggcaaatgcc aaaaacaaag aaggcgcgct gaaattgatc
    14761 aacttcctgc tgcgcccgga tgtggcaaaa caggttgctg aaactatcgg ttatccaacg
    14821 ccaaaccttg cggcgcgtaa gctgttaagt ccagaagtgg cgaacgataa aacgctctac
    14881 cccgatgctg aaaccattaa aaatggcgaa tggcagaatg acgttggcgc agccagcagc
    14941 atttatgaag agtattatca gaagctgaaa gcaggacgtt aattcagcaa gcgggcggtg
    15001 gcattaccgc ccgtttttta tttagcaagg atgtggctaa tgagtcagga cagtaaagtt
    15061 ttatcccgcg cttttctcgg tatcggtctg atattgctcg ttatttctgc aatcatcttt
    15121 tattaccatt ttacttttac caaaagcgcg gttcatacag aaggaatcat tgttgatgcc
    15181 gtttggtaca acaatcactc aaacgacgtc gatgataatg gttcctggta tccagtggtt
    15241 gcctttcgcc ctacgccaga ttacacgcta attttcaatt cgaatatcgg tagtgatttt
    15301 tatgaagata gcgaaggtga cagagtcaat gtctattatc cgccagggca tccagaacaa
    15361 gccgaaataa acaatccctg ggttaatttt tttaaatggg gctttgttgg catagcaggc
    15421 attattttcg gtagtgtcgg tctgataatc acgctcccct ccaccaaaaa atctcgcagg
    15481 aaacggaagt cctgcccatg aaaaaattca ttatactgct cagtttactg atactcctgc
    15541 ctctggtagc aaccagtaaa ccattgattc caataatgaa gacacttttt acggatgtta
    15601 ccggtactgt accggatgct gaagagatcg cacgtaaagc cgagcttttt cgccaacaaa
    15661 cgggaattgc accgtttatt gtgattttac ccgacattaa taatgaagcc agtctcagac
    15721 aaaatggtaa agcgatgctg gcgcatgcgt catcttcatt gagtaatgta aaaggaagtg
    15781 ttctgttact atttactacc cgcgaaccac ggttaattat gatcaccaac ggccaggttg
    15841 aaagtggtct ggacgataaa catctcggcc ttctgataga aaatcacacg ctggcttatt
    15901 taaatgcaga tctctggtat cagggaatca ataatgcatt ggctgttcta caagcacaga
    15961 tattaaaaca accgacgccg ccactgacgt attatccgca tccaggacaa cagcatgaaa
    16021 atgctccccc cggcagcacc aacacattag gttttatcgc ctgggctgcg acgtttatct
    16081 tattttcctg gatcttttgc tacacgactc gttttattta tgcgttaaaa tttgcagtgg
    16141 cgatgactat tgccaatatg ggttgtcagg ctttatgttt gtatatcgat aatagctttg
    16201 cgattaccag aatatcgcca ctctgggcag ggctaattgg tgtctgtacg tttattgccg
    16261 cattgttatt gacgagtaaa cgataaaaga ggccggaaaa gtgcgcgtgt attatgccgg
    16321 atggggcgcg aacgccttat ccggcccaca aaacccgcaa attcaattaa ttgcgtcccc
    16381 ttgtaggcct gataagcgta gcgcatcagg caatgcttcc tgctttataa tcccttcagc
    16441 aacttttcaa caaactcagg caccacctgg cttgccgggc cgtaatattt ctcggcaaat
    16501 tcattaccga cctgacttgg ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc
    16561 gcttcgtgaa caaacccagc cgccggataa acatgcccgg aagtaccaat ggcaatgaaa
    16621 atatcagcca tcgacaacgc catataaatt tcatccatgc cgagtggcat ttcgccaaac
    16681 caaacgacgt gcgggcgcaa gggtgccggg aactggcaac aatggcattt atcttctggc
    16741 gtaacgtctc cggtccagtc gagaacctga ccactttgcg aacaacgaac tttcagcaat
    16801 tccccatgca tatgaatgac attggtatta cctgcgcgtt catgcaggtt gtctatattc
    16861 tgcgttacca gcaaaaagcg atcgccgagg gcgtcttgca gtttagccag cgcaagatgc
    16921 gcggcgttag gctgaatttc tggctgctgc agctgtcgac gacgggcgtt ataaaacgct
    16981 tgcaccagtt caggatcgcg atcgaaacct tccggcgttg ccacatcttc aacccgatgt
    17041 tcttcccaca ggccatctgc ggcgcgaaag gtacgaatac ctgattccgc agaaatccct
    17101 gcccctgtca gtacgagtac tcttggtttt tccattgctt ccggcaccac tttatctctg
    17161 aaaaaaatac gctgacgcaa acgctcgcgc aggcggcgtt tatttttacg aaaacgactt
    17221 aaccgatgac cccgacgcga cagcatagca acctctgttg tttaatcggt tagatgtagg
    17281 aaggccgcac cacgcattcc tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca
    17341 cgagctacag gtaagagatg acgaggcagc ctgtccgcca gttgcgttgt gattgccggg
    17401 aaattcgata agccaccacc aatgacgacc aggtcagggt caacaatggt caggatattt
    17461 cccagacaaa ccgctaataa atccagataa cgctcaacgt gcgcccttgc ctgctcatcg
    17521 ccttgatcat aaagcgcaat aatttcggga gcctgcaacg gttgatgata atagtgttga
    17581 tacagccacg caaaaccgcg accagacaga taattttcaa tgcagccatg ctgaccacag
    17641 ccgcagcggc gtaacgggaa atccaacccc atcatggtta acgcatcaac cggcagacgc
    17701 atatggccaa actcgccggt aatgtagctt ttaccggtaa tcggtttgcc gttgaaaatc
    17761 agcccgccgc caacgccggt gccgagaatc aaccccatca ccagtggata ttgagtaaat
    17821 tcatcatccc aggcttctga aagggcaaaa cagttggcat cgttatcaag gcgtacatcg
    17881 cgatcaagac gtgcgctcag gtcggcacgc agcggtttac cgctggcagc ggggacattg
    17941 gcggcataca gcgtaccatc ttctgtttcc ggcattcccg gaataccgat gccgacagaa
    18001 cctttacagc caaaacgttg gtcagcttca gctaccagct cacacactgc atctaaaaat
    18061 gcgtcatagc tgtcacgcgg tgtcggcacc cgcttttccc actgcaactg ccgaccgcta
    18121 tcaaacacgc caagcgcaat ttttgttcca ccaatatcaa acccgtaata cattgccgct
    18181 ccttgtttta atgtactgcc tttactggcc gctaaggact cgcgcagggt caatattact
    18241 ggcgcgccgc gccggatacc aacttgccaa aagactcagc aacaatgctg tgaccagtac
    18301 gtagaagacg tccagccagt gcaattccga tggcaggaaa tcaataaaat agatatcgct
    18361 ggagaggaac tgatgaccga tcaacttttc aatccactca ataatcgggg taagttgcag
    18421 tgaaaccacc acaccgataa tcaccccaca caggctgccg aacagccctg ccagcaatcc
    18481 ataccagaca aagatggcgc gaattaaacc atctttcgcc cccagcgttc ttaatactgc
    18541 gatatcgcca ctcttgtctt tcaccgccat cactaaggtg gagacgatgt tgaaacaggc
    18601 cacgccaatc accagtacca tcgccagata cataatggcg cggatcatct ggatatcgcg
    18661 atacatatag ccgtaagtac caatccaact tttaatataa acatagctgt tggtcacttc
    18721 acccgcatcg cgtaccagct tattggcgtt gaaaacatcc gtcattttaa gggcaatacc
    18781 tgacacgctg gaacccatat caagatattg ttgggcatcc gccagcggga tcatggcaaa
    18841 actgtgatcg agttgaccac tcaactgcaa aataccggca acgtgcaaac gcacacgttt
    18901 tggctgcatc agtttatgct cgggattcga gttggggatc ataatcgaca cccaatcccc
    18961 ctgcttcact ttcagcgcat ccgccacgcc tttgccgatg ataatttgct gttcgcccgc
    19021 tttaaaattg cgccacgcat cgccctggac aaacgagggt aatgcgctca gacgctgttc
    19081 ctgttgcggg ttaacgccct tcacctggat tgcgcgaaga ttcgcgccac tttccaccag
    19141 cccggtgaaa ttgatatacg gcgcggcagc ggcaataccc ggcacttttt gcacgtgatc
    19201 cagtgcttcc tgccagttag tccacggctg atccaccgcc tcgatttcac catgcggcac
    19261 caccgccaga atacggttgt tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac
    19321 gatcaatacc gccacgccga gggcaatgcc aatggtagaa ataacggaga tcagcgacac
    19381 catgccgccg cgccgccgtc cgcgactaaa acgcaggcca attaataacg ataaaggcat
    19441 cgccattact ccgcccccat caggctcagt tccgccgtca gacgaccatc acgcatttct
    19501 aactggcggc tcatacgttt cgccagttgc aggtcgtgag taaccaccag gaaggcggtg
    19561 ccctgcaagc gattcaattc cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg
    19621 aggttaccgg taggttcatc cgccagtacc aggcgcgggt tattcaccag cgcacgggca
    19681 atcgccacac gctggcgttc gccgccagaa agttcagatg ggcggtgatt cgcacgatga
    19741 tccagcccca ccgcttttaa catctcaagt gcacggctgt tgatttcagc gggctttttc
    19801 ttgccaatca gcagcggcat agccacgttt tccagggcgg taaaatccgg cagcaggtgg
    19861 tgaaactgat aaataaagcc cagcttctgg ttgcgcagct ctgctttcgc cgccgaagac
    19921 agtttgctca tcggttgacc gttaaagatc acatcgccgg aggttggcgt atccagcccg
    19981 cccaacaagt gcagcaaggt gcttttaccg gaaccagagc taccgacgat cgccatcatt
    20041 tcaccttcgc cgacgctgaa actgacattg tgcagcacat cggtttgcac actgccttcc
    20101 tgatagcgtt tgcacaggtt gtcgcattgc aacaggatct tattcataac gtaaagcctc
    20161 agcgggttga gtggcggcag cgcgccatga agggtaaagc gtagacagca gcgcgatagc
    20221 catcgccacc agcgcaataa caatgacctg taaaggttcg atagcgaccg gcagcgccgc
    20281 gccatcaagc aggacgccga ttatcggcat cagattattt aactggctgg caagcagggc
    20341 gccaagcgcc gctccgagga tcgcaccgat aatcccggcg ctggcccctt gcaccataaa
    20401 gaccatcatg atttgtcgcg gagttaagcc ttgcgtttgc aggatcgcta cttcgccctg
    20461 cttctccatt accatcagcc ctagtgaggt aataatgtta aacgccgcaa cggcgacaat
    20521 caggctcagc agtaaaccca tcatattttt ttccatgcgt acggcctgga acaactcgcc
    20581 tttacgatca cgccagtcct gccatttgct gccttcaggc agtttttgct gacttaatga
    20641 gtcgactttc agcggctcat ccagccacaa acgccagccg gtaatattgc ctgccggata
    20701 acgcatcaga cgcgaggcat cctcaatatt caccagcatt tcattgccat cgacttcact
    20761 gttggcggcg aaagtgccaa tcacattgaa caggcgctga cttggaatac gccccatcgg
    20821 cgtgaactgg ctggcagatg gtaccatcat gcggatttga tcaccgcgat taacgcctag
    20881 ctgtgaggca agttgttcgc cgaggatgac attatatttc cccggctcga ggtcagtttg
    20941 tttcacattg accagatacg gcgttagtgg atctttttgc gccgggtcga taccgagcat
    21001 caccccgacc gccacgctgc gcgcgctttg cagtaccaca tcaccggtag taataggtgc
    21061 gacgcgatta acgccgtcca gtttgactgc cgtttccggg agttgctgcg ggttaagaga
    21121 gccatgctca gaagagagaa ttgcctgtgg catcaggcca aggatgttgt tttgcagctc
    21181 gcgctcaaag ccgttcatca ctgacaatac tgtgaccagc gccatcaccc cgagggtaat
    21241 gccgatggta gaaagccagg agacgaaacg accgaagcga tccgctgcac gcccacgcat
    21301 gtaacgcagg ccaataaata gagcgacagg ttggtacatg aaatccgtct ggttgctgta
    21361 gcaaagacac gagtatataa gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc
    21421 gtcagggcta atctgaaaag cccccgtaaa tcaatcggct aaattgccat gttttatcat
    21481 caatcttgcc gcctgatctt aatctgaaaa atcatccaat acagacagat acccattaca
    21541 cctcgggtca taacccgcag gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg
    21601 atgaaacaaa aagagctatg gattaaccag atcaaagggt tatgtatttg tctggtggtg
    21661 atttatcact cggtcattac cttttatccg catctgacca cttttcagca tccgttatcg
    21721 gaagtcctga gcaaatgctg gatctatttc aatctttacc ttgccccctt tcgtatgccg
    21781 gtttttttct ttatttccgg ctatttgatt cgccgctata tcgacagcgt accgtgggga
    21841 aattgtctcg ataaacggat ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg
    21901 cagtggctgg cgttaagtgc gctgaaccag tggctggcac ctgagcgcga tttaagcaat
    21961 gcctccaatg ccgcttatgc cgattccacc ggtgagttcc tgcacgggat gatcaccgcc
    22021 agcaccagct tgtggtatct gtatgcgtta attgtctatt tcgtggtatg taaaattttt
    22081 agccgtctgg cgctgccact attcgccttg tttgtactgc tgagtgtggc ggttaatttc
    22141 gttcccacgc cgtggtgggg aatgaacagt gtgatccgca atttgcttta ttacagcctt
    22201 ggcgcatggt ttggcgcaac aataatgacc tgtgttaaag aggtgccgtt gcgccgccat
    22261 ctgctgatgg cttctttgct taccgttctg gcggtcggtg cctggttgtt tactatctcg
    22321 ctgctgttgt cgctggtatc gattgtggtg atcatgaagc tgttttatca atacgagcaa
    22381 cgattcggta tgcgctccac cagcctgctg aatgtgattg gctccaacac cattgctatc
    22441 tacaccaccc atcgcattct ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac
    22501 gcagcacgct ggtcgccgca agtcgaactg acactcctgc tggtttatcc ctttgtcagt
    22561 ttgttcatct gtactgttgc gggcttgctg gtaagaaaac tttcacagcg tgcattcagc
    22621 gatctgttgt tctccccgcc ttcactgccc gctgccgtca gttactctcg ttaaaccatc
    22681 gccccagccg gggcgattca atttgctaaa ccatgtcgat tacggataat gaacagcatt
    22741 gcttatcaga gatatgcccc catatgttga ggcatatcct aacgagaatc tgacaaccgt
    22801 tatgcctgaa caatatcgtt atacgctgcc cgtcaaagcg ggtgagcagc gtctgctggg
    22861 cgagttaacc ggcgcagcct gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg
    22921 tccggtggta ctcattgcac cagatatgca aaatgctctg cgtttgcatg atgaaatcag
    22981 ccagttcacc gatcagatgg tgatgaatct ggcggactgg gaaactcttc cctacgacag
    23041 tttttcgcct catcaggaca ttatctcctc gcgcctttcc accctttacc agctaccgac
    23101 gatgcagcgt ggcgtactga ttgttccggt gaatacgctt atgcagcgcg tttgcccaca
    23161 cagttttctc cacggtcatg cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc
    23221 attacgaacc caactggaca gcgccggtta tcgccatgtt gaccaggtga tggagcacgg
    23281 cgaatacgcc acgcgcggcg cgttgctgga tctcttcccg atggggagtg agctgcctta
    23341 tcgtcttgat ttctttgatg atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca
    23401 gcgcacgctg gaggaagtag aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga
    23461 taaagcggca attgaactgt tccgcagcca gtggcgcgat accttcgaag tgaagcgcga
    23521 tccggaacat atttaccagc aagtgagtaa aggcacatta cctgccggga tcgagtactg
    23581 gcagccattg ttcttcagcg aaccactgcc gccgctgttc agttatttcc ctgccaatac
    23641 cttgctcgtg aatactggcg atctggaaac cagtgccgaa cgtttccagg ctgacacgct
    23701 ggcgcgtttt gagaatcgcg gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct
    23761 ctggctgcgg gtggacgagc tcttctcaga gctgaaaaac tggccccggg tgcagctaaa
    23821 aactgaacat ttaccgacaa aagccgcgaa tgccaattta ggtttccaga aactgccaga
    23881 cctggccgtt caggcacaac aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac
    23941 tttcgacggt ccggtggtgt tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga
    24001 actgctcgca cgaattaaaa ttgctccgca acgcattatg cgtcttgatg aagccagcga
    24061 ccgtgggcgt tatctgatga ttggcgctac cgaacatggt tttgtcgata aggtgcgtaa
    24121 tctggcgctg atctgcgaaa gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga
    24181 ttctcgccgc accatcaacc ccgatacact gatccgtaac cttgcggagc tgcatattgg
    24241 tcagccggtt gtccatctgg agcacggtgt cgggcgctat gccggaatga ccacgctcga
    24301 agcgggcggc attactggcg agtatttgat gctcacctat gccaacgacg ccaaactgta
    24361 tgttccggtg tcgtcactgc atctgattag ccgttacgca ggtggcgcgg aagaaaacgc
    24421 cccgctgcat aaacttggcg gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa
    24481 agtgcgtgat gtggcggcgg aattgctgga tatctacgcg caacgcgccg ccaaagaggg
    24541 cttcgcgttt aaacacgatc gtgagcagta tcagttgttc tgcgacagct tcccgtttga
    24601 aaccacgccg gatcaggcgc aggccattaa tgcggtactt agcgacatgt gtcagccgct
    24661 ggcaatggat cgtctggtgt gcggcgatgt tggctttggt aaaacagaag tggcgatgcg
    24721 cgccgctttc ctggcagtag ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct
    24781 tctcgcgcag cagcattacg acaacttccg cgaccgtttc gccaactggc cggtacgtat
    24841 cgaaatgatc tcccgtttcc gcagcgccaa agagcagacg caaatccttg cggaagtggc
    24901 ggaagggaaa atcgatattc tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt
    24961 taaagattta ggcctgctga ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga
    25021 gcgcattaaa gcgatgcgcg cgaacgtgga tattctgacg cttactgcaa cgccgatccc
    25081 acgcacgctg aatatggcaa tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc
    25141 cgcccgtcgt ctggcagtta aaacctttgt ccgtgagtat gacagcctgg tggtccggga
    25201 ggcgatcctg cgtgaaattt tgcgcggggg gcaggtttat tatctctaca atgatgtgga
    25261 aaacatccag aaagctgccg aacggctggc agaactggtg cctgaagcac ggattgccat
    25321 cggtcacggg cagatgcgcg agcgcgaact ggaacgggtg atgaatgatt tccatcatca
    25381 acgtttcaac gtgctggttt gtacaaccat tatcgaaacc gggatcgaca tcccgacagc
    25441 caacaccatt atcattgaac gcgcggatca cttcggtctg gcgcagctgc accagttacg
    25501 cggtcgcgtc ggacgttcac atcatcaggc atatgcatgg ctgctgacgc cgcatccaaa
    25561 agcgatgact accgatgcac aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg
    25621 tgcaggcttt gcgctggcaa cgcacgatct ggagatccgc ggcgcgggtg aactgcttgg
    25681 cgaagaacaa agtggatcaa tggaaaccat cggtttctcg ctgtatatgg agttgctgga
    25741 aaacgccgtc gatgcactga aatccggacg cgagccgtcg ctggaagatc tcaccagcca
    25801 gcaaacagaa gtcgagctgc ggatgccgtc gctattgcca gatgatttca ttcctgacgt
    25861 gaatacgcgt ttgtcgttct ataaacgtat tgccagcgcc aaaacggaaa acgaactgga
    25921 agagatcaaa gtcgagctta tcgatcgctt cggcctgctg ccggatccgg cgcgtaccct
    25981 gctggatgtt gcccgtctgc gccagcaagc gcagaaactg gggatcagga agctggaagg
    26041 taatgagaaa ggcggggtga tcgaatttgc cgagaagaat cacgttaatc cggcctggtt
    26101 gattggtttg ctgcaaaaac agccgcagca ttaccgcctt gatggtccga cgcgcctgaa
    26161 atttattcag gatttgagtg agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg
    26221 tgaactggaa gagaacgcga tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc
    26281 tgcaacattt acaaattctt tgcacttccc tgcactatcc gacactgtca ccatccataa
    26341 ttcaggctta tctgtttatt acaataacct tatatttatt atggattttt ggtgatgatc
    26401 aaaacgcgtt tttctcgctg gctaacgttt tttacgttcg ccgctgccgt ggcgctggcg
    26461 ctaccggcaa aagccaacac ctggccgctg ccgccagcgg gcagtcgtct ggttggcgaa
    26521 aacaaatttc atgtggtgga aaatgacggt ggttctctgg aagccatcgc caaaaaatat
    26581 aacgtcggct ttctcgctct gttacaggct aaccctggcg ttgatcctta cgtaccgcgc
    26641 gcgggtagcg tgttaacgat cccgttgcaa accctacttc cagatgcgcc gcgcgaaggc
    26701 attgtgatca atattgcgga gctgcgtctc tattactacc cgccgggtaa aaattcggta
    26761 accgtgtatc caataggtat tggtcagtta ggtggtgaca cgctgacacc gacaatggtg
    26821 accaccgttt cagacaaacg tgcaaaccca acctggacgc caacggcaaa catccgcgcc
    26881 cgttataaag cacagggaat tgagttgcct gcggtagtgc cggctggacc ggataaccca
    26941 atgggccatc atgcgattcg tctggcggcc tatggcggcg tttatttgct tcacggtacg
    27001 aacgccgatt tcggcattgg catgcgggta agttctggct gtattcgtct gcgggatgac
    27061 gatatcaaaa cactctttag ccaggtcacc ccaggcacca aagtgaatat catcaacact
    27121 ccgataaaag tctctgccga accaaacggt gcgcgtctgg ttgaagtaca tcagccgctg
    27181 tcagagaaga ttgatgacga tccgcagctg ctgccaatta cgctgaatag cgcaatgcaa
    27241 tcatttaaag atgcagcaca aactgacgct gaagtgatgc aacatgtgat ggatgtccgt
    27301 tccgggatgc cggtggatgt ccgccgtcat caagtgagcc cacaaactct gtaaatcctg
    27361 gataaaaaaa atccccgcga cacggcatgt cgcggggaaa aaatcagcag tagcaggcat
    27421 taatgagggt taatgcttat ttataaatta ctgctgttcc atggagggta ttcggaccgg
    27481 ttacagaagt aatacggaaa gattttgcgc ccatctcatc cgctttttgc gccagctgct
    27541 cttccagcga tcccagattt gtccccgcgt tagcactgat tgtaccgact ttttgttggc
    27601 cttctggcgt tgactgaact tcgacagccg caaagctggc aaatgacatg gagcttaaaa
    27661 tcgccgcagc gatgagggtt tttacgtttt tcataatagt ggccttatgc agatgaatga
    27721 cgtcgcaagc aattacttaa cgatcgataa ctaaatgata gatgtga
//
LOCUS       DAZFRN010000056        27221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFRN010000056 DAZFRN010000000
VERSION     DAZFRN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.056"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..212
                     /locus_tag="ACHKUB_002306"
     CDS             <1..212
                     /locus_tag="ACHKUB_002306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655318.1"
                     /translation="RIPEDPHHWNGFSGEKRILYGHSAHHFFFSSSTIHVSLPRLITD
                     YEVQKYLSFQLPVIRYRERFWQYME"
     gene            219..2642
                     /gene="pgaA"
                     /locus_tag="ACHKUB_002307"
     CDS             219..2642
                     /gene="pgaA"
                     /locus_tag="ACHKUB_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO6655319.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2651..4669
                     /gene="pgaB"
                     /locus_tag="ACHKUB_002308"
     CDS             2651..4669
                     /gene="pgaB"
                     /locus_tag="ACHKUB_002308"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO6655320.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4662..5987
                     /gene="pgaC"
                     /locus_tag="ACHKUB_002309"
     CDS             4662..5987
                     /gene="pgaC"
                     /locus_tag="ACHKUB_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO6655321.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5989..6402
                     /gene="pgaD"
                     /locus_tag="ACHKUB_002310"
     CDS             5989..6402
                     /gene="pgaD"
                     /locus_tag="ACHKUB_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO6655322.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6452..7240)
                     /gene="phoH"
                     /locus_tag="ACHKUB_002311"
     CDS             complement(6452..7240)
                     /gene="phoH"
                     /locus_tag="ACHKUB_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO6655323.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7860..9131)
                     /gene="efeB"
                     /locus_tag="ACHKUB_002312"
     CDS             complement(7860..9131)
                     /gene="efeB"
                     /locus_tag="ACHKUB_002312"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO6655324.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9137..10264)
                     /gene="efeO"
                     /locus_tag="ACHKUB_002313"
     CDS             complement(9137..10264)
                     /gene="efeO"
                     /locus_tag="ACHKUB_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO6655325.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10322..11152)
                     /gene="efeU"
                     /locus_tag="ACHKUB_002314"
     CDS             complement(10322..11152)
                     /gene="efeU"
                     /locus_tag="ACHKUB_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO6655326.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALPRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11694..13202)
                     /gene="putP"
                     /locus_tag="ACHKUB_002315"
     CDS             complement(11694..13202)
                     /gene="putP"
                     /locus_tag="ACHKUB_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO6655327.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13361..13570)
                     /locus_tag="ACHKUB_002316"
     CDS             complement(13361..13570)
                     /locus_tag="ACHKUB_002316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655328.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13625..17587
                     /gene="putA"
                     /locus_tag="ACHKUB_002317"
     CDS             13625..17587
                     /gene="putA"
                     /locus_tag="ACHKUB_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6655329.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17627..18265)
                     /gene="rutR"
                     /locus_tag="ACHKUB_002318"
     CDS             complement(17627..18265)
                     /gene="rutR"
                     /locus_tag="ACHKUB_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO6655330.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18553..19644
                     /gene="rutA"
                     /locus_tag="ACHKUB_002319"
     CDS             18553..19644
                     /gene="rutA"
                     /locus_tag="ACHKUB_002319"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO6655331.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19644..20336
                     /gene="rutB"
                     /locus_tag="ACHKUB_002320"
     CDS             19644..20336
                     /gene="rutB"
                     /locus_tag="ACHKUB_002320"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO6655332.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20348..20734
                     /gene="rutC"
                     /locus_tag="ACHKUB_002321"
     CDS             20348..20734
                     /gene="rutC"
                     /locus_tag="ACHKUB_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO6655333.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20742..21542
                     /gene="rutD"
                     /locus_tag="ACHKUB_002322"
     CDS             20742..21542
                     /gene="rutD"
                     /locus_tag="ACHKUB_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO6655334.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQAMVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21552..22142
                     /gene="rutE"
                     /locus_tag="ACHKUB_002323"
     CDS             21552..22142
                     /gene="rutE"
                     /locus_tag="ACHKUB_002323"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO6655335.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            22153..22647
                     /gene="rutF"
                     /locus_tag="ACHKUB_002324"
     CDS             22153..22647
                     /gene="rutF"
                     /locus_tag="ACHKUB_002324"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO6655336.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22668..23996
                     /gene="rutG"
                     /locus_tag="ACHKUB_002325"
     CDS             22668..23996
                     /gene="rutG"
                     /locus_tag="ACHKUB_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO6655337.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24079..24252)
                     /gene="ymdF"
                     /locus_tag="ACHKUB_002326"
     CDS             complement(24079..24252)
                     /gene="ymdF"
                     /locus_tag="ACHKUB_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6655338.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24625..25221
                     /gene="wrbA"
                     /locus_tag="ACHKUB_002327"
     CDS             24625..25221
                     /gene="wrbA"
                     /locus_tag="ACHKUB_002327"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6655339.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25242..25469
                     /gene="yccJ"
                     /locus_tag="ACHKUB_002328"
     CDS             25242..25469
                     /gene="yccJ"
                     /locus_tag="ACHKUB_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO6655340.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25507..26748)
                     /gene="agp"
                     /locus_tag="ACHKUB_002329"
     CDS             complement(25507..26748)
                     /gene="agp"
                     /locus_tag="ACHKUB_002329"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO6655341.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27041..>27221)
                     /locus_tag="ACHKUB_002330"
     CDS             complement(27041..>27221)
                     /locus_tag="ACHKUB_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655342.1"
                     /translation="SISLGNESFSTDDDLEYGYLINAGNHYDVYLPPELFAQAYKLKN
                     KEMNAQLDYLNRYAI"
BASE COUNT         6642 a         7050 c         6891 g         6638 t
ORIGIN      
        1 gccgtattcc tgaagatcct catcattgga atggattttc gggcgagaaa aggattttat
       61 atggacactc tgctcatcat ttcttcttct catcatcaac aattcacgtc tctcttccgc
      121 gtttaataac ggattatgag gtgcaaaaat atctttcttt tcagttacct gtaattagat
      181 acagagagag attttggcaa tacatggagt aatacaggat gtattcaagt agcagaaaaa
      241 ggtgcccgaa aaccaaatgg gctttgaaac ttcttactgc cgcattttta gcagcgagtc
      301 ccgcggcgaa gagtgctgtt aataacgcct atgatgcatt gattattgaa gctcgcaagg
      361 gtaatactca gccagctttg tcatggtttg cactaaaatc agcactcagc aataaccaaa
      421 ttgctgactg gttacagatt gccttatggg ccgggcaaga taaacaggtt attaccgttt
      481 acaaccgcta ccgtcatcag caattaccag cgcgtggtta tgcagctgtc gccgtcgctt
      541 atcgtaacct gcaacaatgg caaaactcgc ttacactgtg gcaaaaggcg ctctctctgg
      601 agccgcaaaa taaggattat caacggggac aaattttaac cctggcagat gctggtcact
      661 atgatactgc gctggttaaa cttaagcagc ttaactctgg agcaccggac aaagccaatt
      721 tactcgcaga agcctatatc tataaactgg cggggcgtca tcaggatgaa ttacgggcga
      781 tgacagagtc attacctgaa aatgcatcta cgcaacaata tcccacagaa tacgtgcagg
      841 cattacgtaa taatcaactt gctgccgcga ttgacgatgc caatttaacg ccagatattc
      901 gcgctgatat tcatgccgaa ctggtcagac tgtcgtttat gcctacgcgc agtgaaagtg
      961 aacgttatgc cattgccgat cgcgccctcg cccaatacgc tgcattagaa attctgtggc
     1021 acgataaccc agaccgtact gcccagtacc agcgtattca ggttgatcat cttggcgcgt
     1081 tattaactcg cgatcgttat aaagacgtta tttctcacta tcagcgatta aaaaagacgg
     1141 ggcaaattat tccgccctgg gggcaatatt gggttgcatc ggcttatctc aaagatcatc
     1201 agccgaaaaa agcacagtca ataatgaccg agctctttta tcacaaggag accattgccc
     1261 cggatttatc cgatgaagaa cttgcggatc tcttttacag ccacctggag agtgaaaatt
     1321 atccgggcgc gctaactgtc acccaacata ccattaatac ttcgccgcct ttccttcggt
     1381 taatgggcac gcctacgagc atcccgaatg atacctggtt acaggggcat tcgtttctct
     1441 caaccgtagc aaaatatagt aatgatcttc ctcaggctga aatgacagcc agagagcttg
     1501 cttataacgc accaggaaat cagggactgc gcattgatta cgcgagtgtg ttacaagccc
     1561 gcggttggcc tcgtgcagca gaaaatgaat taaaaaaagc agaagtgatc gagccacgta
     1621 atattaatct ggaggttgaa caagcctgga cagcattaac gttacaagaa tggcagcagg
     1681 cagctgtctt aacgcacgat gttgtcgaac gtgaaccgca agatcccggc gttgtacgat
     1741 taaaacgtgc ggttgatgta cataatcttg cagagcttcg tatcgctggc tcaacaggaa
     1801 ttgatgccga aggcccggat agtggtaaac atgatgtcga cttaaccacc atcgtttatt
     1861 caccaccgct gaaggataac tggcgcggtt ttgctggatt cggttatgcc gatggacaat
     1921 ttagcgaagg aaaagggatt gttcgcgact ggcttgcggg tgttgagtgg cggtcacgta
     1981 atatctggct cgaggcagag tacgctgaac gcgttttcaa tcatgagcat aaacccggcg
     2041 cgcgcctgtc tggctggtat gattttaatg ataactggcg tattggttcg caactggaac
     2101 gcctctctca ccgcgttcca ttacgggcaa tgaaaaatgg tgttacaggc aacagtgctc
     2161 aggcttatgt tcgctggtat caaaatgagc ggcgtaagta cggtgtctcc tgggctttca
     2221 ctgatttttc cgacagtaac cagcgtcatg aagtctcact tgagggtcag gaacgcatct
     2281 ggtcttcacc atatttgatt gtcgatttcc tacccagtct gtattacgaa caaaatacag
     2341 aacacgatac cccatactac aaccctataa aaacgttcga tattgttccg gcatttgagg
     2401 caagccattt gttatggcga agctatgaaa atagctggga gcaaatattc agcgcaggtg
     2461 ttggtgcctc ctggcaaaaa cattatggca cggatgtcgt cacccaactc ggctacgggc
     2521 aacgcattag ctggaatgac gtgattgatg ctggcgcaac gctacgctgg gaaaaacgac
     2581 cttatgacgg tgacagagaa cacaacttat acgttgaatt cgatatgaca ttcagatttt
     2641 aaggataaat atgttacgta atggaaataa atatctcctg atgctggtga gtataattat
     2701 gctcaccgcg tgcattagcc agtcaagaac atcatttata ccgccacagg atcgcgaatc
     2761 tttactcgcc gagcaaccgt ggccgcataa tggttttgta gcgatttcat ggcataacgt
     2821 tgaagacgaa gctgccgacc agcgttttat gtcagtgcgg acatcagcac tgcgtgaaca
     2881 atttgcctgg ctgcgcgaga acggttatca accggtcagt attgctcaaa ttcgtgaagc
     2941 acatcgagga ggaaaaccgc taccggaaaa agctgtagtg ctgacttttg atgacggcta
     3001 ccagagtttt tatacccgcg tcttcccaat tcttcaggcc ttccagtggc ctgctgtatg
     3061 ggcccccgtc ggcagttggg tcgatacgcc agcggataaa caagtaaaat ttggcgatga
     3121 gttggtcgat cgagaatatt ttgccacgtg gcaacaagtg cgagaagttg cgcgttcccg
     3181 gctcgttgag ctcgcttctc atacatggaa ttctcactac ggtattcagg ctaatgccac
     3241 cggcagctta ttgcctgtat atgtaaatcg tgcatatttt actgaccacg cacggtatga
     3301 aaccgcagca gaataccggg aaagaattcg tctggatgct gtaaaaatga cggaatacct
     3361 gcgtacaaag gttgaggtaa atccacacgt ttttgtttgg ccttatggcg aagcgaatgg
     3421 catagcgata gaggaattaa aaaaactcgg ttatgacatg ttcttcaccc ttgaatcagg
     3481 tttggcaaat gcgtcgcaat tggattccat tccgcgggta ttaatcgcca ataatccctc
     3541 attaaaagag tttgcccagc aaattattac cgtacaggaa aaatcaccac aacggataat
     3601 gcatatcgat cttgattacg tttatgacga aaacctccag caaatggatc gcaatattga
     3661 tgtgctaatt cagcgggtga aagatatgca aatatcaacc gtgtatttgc aggcatttgc
     3721 tgatcccgat ggtgatgggc tggtcaaaga ggtctggttt ccaaatcgtt tgctaccaat
     3781 gaaagcagat atttttagtc gggttgcctg gcaattacgt acccgctcag gtgtaaacat
     3841 ctatgcgtgg atgccggtat taagctggga tttagatccc acattaacgc gagtaaaata
     3901 cttaccaaca ggggagaaaa aagcacaaat tcatcctgaa caatatcacc gtctctctcc
     3961 tttcgatgac agagtcagag cacaagttgg catgttatat gaagatcttg ccggacatgc
     4021 tgcttttgat ggcatattgt tccacgatga tgctttgctt tcagattatg aagatgccag
     4081 tgcaccggct atcacggctt atcagcaagc aggctttagc gggagtctga gcgaaattcg
     4141 acaaaacccg gagcaattta aacagtgggc ccgctttaaa agtcgtgcgt taactgactt
     4201 cactttagaa cttagtgcgc gcgtaaaagc cattcgcggt ccacatatta aaactgcacg
     4261 aaatattttt gcacttccgg taatacaacc tgaaagtgaa gcctggtttg cacagaatta
     4321 tgctgatttc ctaaaaagct atgactggac cgctattatg gctatgcctt atctggaagg
     4381 tgtcgcagaa aaatcggctg accaatggtt aatacaattg accaatcaaa ttaaaaacat
     4441 ccctcaggct aaagacaaat ctattttaga attacaggca caaaactggc agaaaaatgg
     4501 tcagcatcag gctatttctt cgcaacaact cgctcactgg atgagcctat tacaactgaa
     4561 tggagtgaaa aactatggtt attatcccga caattttctg cataaccaac ctgaaataga
     4621 ccttattcgt cctgagtttt caacagcctg gtatccgaaa aatgattaat cgcatcgtat
     4681 cgttttttat attatgtctg gtgttatgca tacccctatg cgtagcgtac tttcactctg
     4741 gtgaactgat gatgaggttc gttttcttct ggccgttttt tatgtccatt atgtggattg
     4801 ttggcggcgt ctatttctgg gtctatcgtg aacgccactg gccgtgggga gaaaacgcac
     4861 cagctcccca gttgaaagat aatccgtcta tctccattat cattccctgt tttaatgagg
     4921 agaaaaacgt tgaggaaacc atacacgccg ctttagcaca gcgttatgag aacattgaag
     4981 ttattgccgt aaatgacggt tcaacagata aaacccgtgc catcctggat cgcatggctg
     5041 cacaaattcc ccatttgcgg gtcattcatc tggcgcaaaa ccaggggaaa gccattgcgc
     5101 ttaaaaccgg agctgccgcg gcgaaaagtg aatatctggt gtgcattgat ggcgatgcgt
     5161 tattagaccg cgatgcggcg gcatatattg tggaaccgat gttgtacaac ccgcgtgtgg
     5221 gtgccgtaac cggtaatcct cgtattcgaa cacgttctac cctggtgggt aaaattcagg
     5281 ttggcgagta ttcctcaatt attggtttga tcaagcgaac ccagcgtatc tatggaaacg
     5341 tatttaccgt ttccggtgtt attgccgcat ttcgtcgcag cgccctggca gaagtgggtt
     5401 actggagtga cgatatgatc accgaagata ttgatattag ctggaagctg cagttgaatc
     5461 agtggacgat tttttacgag ccacgggcgc tgtgctggat attaatgcct gaaacgttaa
     5521 aagggctgtg gaaacagcgc ctgcgctggg ctcagggcgg tgcagaagta ttcctcaaaa
     5581 atatgacaag gttgtggcgc aaagaaaact ttcgaatgtg gccgctgttt tttgaatact
     5641 gcctgacgac aatatgggcc ttcacctgcc tggtcggttt cattatttac gcagtccaac
     5701 ttgccggtgt accgttaaat attgaattga cacatatcgc tgcgacacat actgccggaa
     5761 tattattgtg tacgttatgt ttactgcaat ttattgtcag cctgatgatc gagaatcgct
     5821 atgagcataa tctgacttca tcgcttttct ggattatttg gttcccggtt attttctgga
     5881 tgctgagcct ggcaacgaca ttggtatcat ttacacgagt catgttgatg cctaaaaagc
     5941 aacgcgcccg ttgggtaagt cccgatcgcg ggattctgag aggttaatat gaacaattta
     6001 attattacga cccgacaatc accagtacgt ttactggttg attatgttgc cacaaccatc
     6061 ttgtggacat tatttgcgtt gttcatattc ttattcgcca tggatctgct gacgggttat
     6121 tactggcaaa gcgaggccag aagccgactt cagttctatt ttttgctggc agtggcgaat
     6181 gccgtcgtgt taattgtctg ggcgctgtac aataagctgc gttttcaaaa acagcagcat
     6241 catgcagcct accaatatac gccgcaagaa tatgcagaga gcttagcaat acctgatgag
     6301 ctctatcagc aactacaaaa aagccatagg atgagcgtac acttcaccag ccaggggcaa
     6361 ataaaaatgg ttgtttcaga aaaagcgcta gtccgggcat aaacacccaa aacaaagccc
     6421 ggttcgcccg ggctctgcac cgataataca cttaactgta tgcatgcagc gtacgttggc
     6481 aaagtgccga acgtacgcaa tcctcttttc cgaaccggac gatcccaacc atttcatctt
     6541 cttcgaaacg ttccagcgcg tcacttaatc cggagcacac gccgcgaggc aaatcgcatt
     6601 gcgtgatatc accgttgacg ataaccgtca cgttctcccc gaggcgggtt aaaaacattt
     6661 tcatttgcgc ggcagtcaca ttctgcgcct cgtcaagaat gacgactgca ttttcaaagg
     6721 tacgtccacg catataggcg aacggcgcaa tttccacctt ccctatttcc ggtcgcaggc
     6781 agtactgcat aaaggaagcc cctaagcgcc ggaccagcac gtcgtagacc gggcgaaaat
     6841 agggagcaaa cttttctgcg atatctccag gtaagaagcc aagatcttca tcggcttgca
     6901 gaactggacg ggtgacgata atcctgtcga catccttatg tatcagggct tctgccgctt
     6961 ttgctgcgct gatccaggtt tttccgcacc cggcttcgcc cgtggcgaat atcagctgct
     7021 tactctcaat agccttcaga tagtgcaatt gcgcttcatt tcgcgcgagg atgggcgaag
     7081 tatcgcgact gtcgcgggcc ataccaatgg cttctacgcc gcccatctgc acaagcgagg
     7141 tgaccgattc ttcttcacgc tgcttatggc tgcgcgaatc ccgtctcagc acacgttttg
     7201 cctcgcgacg agctttgatc actgcttttt gtcttcccat ggagagcacc ttgagttgtt
     7261 tgtattcatc acacgcgccg ttggcagcgc gattatgcgc acgaacatca gagggttggc
     7321 ttccttgtaa gccatagttt gcttttggat aaaatgccga aaaacggcta cgcgcaccgt
     7381 ttacggcgtc ggtaacacat gaaaagaaag gatgaggttg aaaatgcaaa gtgacgagat
     7441 gactaccgga ggagaaaact ccgcgagtgg tggcgcgttg attatctaaa acatgtccag
     7501 tacaggacgt taccatccgc gatctccata gtgactgact atcactgccg ggaacttccg
     7561 ctgctactta ataagtacaa cagatctcgc atttattgca acaatatatt tacttatatt
     7621 taactataaa acaccatttc agtgacatta gtttctactg gaaagatgac agagtgatga
     7681 cagatatgaa aaaagctgtg tgctttcagc aggatttgca tcaggcatca gtgcacgatc
     7741 gccgtatgcg gcgtgaacgc cttatccgcc taccgaaccc gcaccgattg taggcataag
     7801 aagacgcgca agcatcgcat caggcatctg cgcaagaacg ccttatccgt ctaaaaacat
     7861 taaacccgca ataacgcgct tccgagataa tcgttcgcgt ccttcactcc cggcagcgca
     7921 aaaaaataac cgccgccgat aggtttaacg tattcctcca gcgcttcgcc attgagcctt
     7981 ttttgtactg tcaggaagcc tttttccaga tcgtgttggt aacagacaaa cagtagcccc
     8041 atatccagtt gcccggagtt ggtgacgccc agtgaataac tgtagccacg acgcagcatc
     8101 aggctggact cactctccgc cgtgcgggga ttcgccagcc ggatatggct gtccagcgca
     8161 atcaccttcc cttccggatc gctggcgtaa tcgggaacat catgctcatg ctgcattccc
     8221 agcggcgcac cggtttgctt atcacgacca aaaatcgtct gctgttcttt cagcggagtt
     8281 ctgtcccaaa attccactcg aaactgaatc aagcgtactg cctgatagct gccaccgatt
     8341 gtccacgctg gctcctgctg atctgccgtt acccacacca ctttttgcat caacttatca
     8401 ttctggctat cgggattggc agtgccgtct ttgaaaccca gcaaattaat cggcgtctct
     8461 ttgcctttac tacgcgccgc gtgatcggaa ataaaccctt cccgcttcca gcgcacgctg
     8521 agcaaatccg gcgtatgttt gatgatatcg cgcagtgcat ggatcaccgt gtcctgggtg
     8581 ttggcgcaaa tttgtagcaa cacatcacca tgacataacg ccgcatccag cgagtcgttg
     8641 ggaaaacgcg tcatcttctg caactttttc ggcatctgtg gcgcaaggcc aaagcgctca
     8701 tcaaacaatg agtgacccac cgataacgtg atggtgagat tatcgggcgc aatgtagccg
     8761 ccaagaatgc cggaatcgag tggtggcagg cgcggatttg gcgtttctgg tgccgctccg
     8821 ccctgagtca gaaaagcaaa acgctgagtc aacaagcgaa acaaccgttc aagatcggct
     8881 ttatcgctgg caagcacatc aaacgccacc agcatcattg cggcctgttg tggcgtcagg
     8941 atccctgcct gatgctcacc ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc
     9001 gtacccggcg cactttgcgt tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc
     9061 agcgcaccaa tgcctttcag taaacgtcgg cgtgacggtt cattcacgcc gtttttatct
     9121 tcatactgca taatgcttaa tccaatccca gcacaccgcg aagttgcgcc agatcttccg
     9181 ccagcgcagt aatcggtcct ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg
     9241 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     9301 catcgacttt tgccaacagt tccgggttgg ctttttgcag ttgtggacgc agcagatcga
     9361 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     9421 gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg gctgcaccgc
     9481 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     9541 cggtataaag ctggtcagcg tactgatcca tgcctttggt ggtgttgtcg ccaaacaatg
     9601 ctttttccag acggtggaaa ccggtgaatt ttggatcagc ggctttttgc tcgtaatcat
     9661 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatgc
     9721 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
     9781 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat
     9841 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
     9901 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
     9961 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga
    10021 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc agcgccttct
    10081 ggctatggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
    10141 cgcactgctt atccgtcacg gtcactttga cctgcgtcac atcagcggcg ttagccataa
    10201 aagcagaaga aaacagcgca gccacgctca actgcaatgc gttacggcgg aagttaatgg
    10261 tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg cgtcgtattt
    10321 gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg
    10381 ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggcgct tcctgatagc
    10441 caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagagcacc gcactcatat
    10501 cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg cgaatggcac
    10561 cagctgccag ccctgcggcg acgaagagaa taaacaggct ggtccattta aaaaatgcac
    10621 caagattgag gcgaataccg ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa
    10681 gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat gccgccagca
    10741 ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc atcaccagcg
    10801 cccagccatg atgatttcca cgcggcaatg cgctatcgac tgcctgctcc agttgtactt
    10861 tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc accacggcga
    10921 tcaccgccac gataccttca aacagttcct gctctttttg cggaaattcg ccggtggttt
    10981 cgttaatgaa gatccccaag cccaggcaca acgcagcggc aagcaacacg ccaatccaca
    11041 tcacaccaat ccatcggcct cgctgggtac gcttaagata gctggcaatc aaactgacaa
    11101 tcagcgcggc ttcaagtcct tcgcgcaaca taatgagaaa cggaacaaac atgccaccca
    11161 cccttaaacg tttttctgag tcaatctatg caaagaaaag taaatcacag tgatagcgat
    11221 tatcattacg gtaaagaaaa atacaagcgg aatgtttata aaatgatgtt gaaatgtaaa
    11281 caaagagagg taatgccagg ggatataaac gattattcca ggtacaaaga tgggattgtt
    11341 tacaacgagc catgtgttct tgccggatgc cgcgtaaacg ccgtatccag ccgacaaaac
    11401 cgtcctgact caatacatca aaacttccat gtaaaactga taagcgggtg tatcaagcaa
    11461 tttcgtccat ccgcgattat caaatctaac tttttgcccg gaacataata acagctcggg
    11521 tgttggtggc gtcaaaatcg ctgaagcgtt ccccgaaggc cgggacagca gggggccgcg
    11581 gcgattggcc gccccctgcg cgctccctgc accattcaat ttatatggcg aggcacatta
    11641 agggagcgca acccgctgct gaagccaaac aaaaccgccg caggctaagt cccttagctt
    11701 tcctgcaacc gtgacggcgg agccgaatga tagtgcgcat cggcctcggc aaagcgtttt
    11761 tgcatcgccg ctgacggcgc tttacccagc aaactaaaca ctacaatccc gatactgccg
    11821 aagatgaagc ccggaataat ttcgtagagt cccagccagc cgaactgctt ccagacgata
    11881 accgtcagcg caccgatgat catccccgcc agcgcaccgt tgcgcgtcat gcgtgaccac
    11941 atcaccgaga acagcaccac cggaccaaac gccgcgccaa agcctgccca cgcgtagctc
    12001 actaagccca gcacgcggtt ttccgggttt gccgccagcg caatcgccac cagcgccacc
    12061 accagcacca tcacacgccc tacccacacc agctctttct ggctggcatg tttacgcaga
    12121 aacgctttgt ataaatcttc ggtaatcgca ctggagcaca ccagcagctg gcaacttaag
    12181 gttgacatta ccgccgccag aattgccgac agcagaatcc cggcaatcca cgggttaaac
    12241 agaatttgcg ccagttcgat aaacacgcgc tcagcattct ggtttaccgc acctgctacc
    12301 gccgggtgct cattaaagta agcaatacca aagaagccga cagctaccgc ccctgccagg
    12361 cagaggatca tccaggtcat actgatacga cgcgcatgga caatgctgtg gtgagagtct
    12421 gccgccataa aacgcgccag gatgtgcggc tgcccgaagt aacccagccc ccagcccatc
    12481 agcgagataa tggcaacaaa gttcagacct ttgagcatat caacgttttc gatgctcttt
    12541 tgtttgatca cttccagcga gtcaccaaag ccaccgacac tgataatgac gataaccggt
    12601 gtcaggatca gggcaaaaat catcaggctg gcctgtacag tatcagtcca gctcaccgcg
    12661 aggaaaccgc caataaaggt gtaaaggatc gtcgccgcag ctcccgccca cagagccgtt
    12721 tcgtagctca tgccaaaggt actttcaaac agacgcgcgc ctgccacaat gcccgaagcg
    12781 caataaatgg tgaagaacag caaaataacc agcgcggaga taatgcgcaa aatgcggctt
    12841 ttatcttcaa agcgcccggt gaaataatcc ggcagggtta aggcgttatt gttgtattcg
    12901 gtatgcacac gcaaccgccc ggccaccagc ttccagttga tccacgcgcc taatgtcagg
    12961 ccaatggcga tccagctttc ggaaatcccg gaaagaaaaa ccgcgcccgg caaccccatt
    13021 aacagccagc cgctcatatc cgacgcaccc gccgataatg ccgtcacgaa tggcccaaga
    13081 ctacgaccgc ccagaatata gtcgtcaaag tttttcgttg atcgccaggc gataaaccca
    13141 atcaatatca tgccaaagat atagacacaa aatgtcacca acatcggtgt gctaatagcc
    13201 atctaaagtc tccaaaaaat tattatcggc aatgtcgaaa cttgccgtta tatctgccac
    13261 cggaacgggg taacagagtt tatgttttac cagggcgacc gtatcctgcc ggaagcgctg
    13321 gttattcaca atcgatttaa cacaccattt acattaaatt ttagtgctca gcgacactat
    13381 ttttcatcag gttgcactct ctcacatttt ttgcggttgc acctttcaaa aatgttaact
    13441 gccgcagaga aaaagtctga gttatttttt taatccctgt catatcgatt tcttttatta
    13501 acatttcatt catttttaag cttgctacgc aggtcacatt taacatggtt gcacaaagtt
    13561 gcaacatcat ggatatttca cgataacgtt aagttgcacc tttcagaaca acaggagtaa
    13621 tggcatggga accaccacca tgggggttaa gctggacgac gcgacgcgtg agcgtattaa
    13681 gtctgccgcg acacgtatcg atcgcacacc acactggtta attaagcagg cgattttttc
    13741 ttatctcgaa caactggaaa acagcgatac tctgccagag ctacctgcgc tgctttctgg
    13801 cgctgccaat gagagcgatg aagcaccgac tccggcagag gaaccacacc agccattcct
    13861 cgactttgcc gagcaaattt tgccccagtc ggtttcccgc gccgcgatca ccgcggccta
    13921 tcgtcgcccg gaaaccgaag cggtttctat gctgctggaa caagcccgcc tgccgcagcc
    13981 agttgctgaa caggcgcaca aactggcgta tcaactggcc gataaactgc gtaatcaaaa
    14041 aaatgccagt ggtcgcgcag gtatggtcca ggggttattg caggagtttt cgctgtcatc
    14101 gcaggaaggc gtggcgctga tgtgtctggc agaagcgttg ttgcgtattc ccgacaaagc
    14161 cacccgcgac gcgttaattc gcgacaaaat cagcaacggt aactggcagt cacacattgg
    14221 tcgtagcccg tcactgtttg ttaatgccgc cacctggggg ctgctgttta ctggcaaact
    14281 ggtttccacc cataacgaag ccatcctctc ccgctcgctg aaccgcatta tcggtaaaag
    14341 cggtgaaccg ctgatccgca aaggtgtgga tatggcgatg cgcctgatgg gcgagcagtt
    14401 cgtcacgggt gaaaccatcg cggaagcgtt agccaatgcc cgcaagctgg aagagaaagg
    14461 tttccgttac tcttacgata tgctgggcga agctgcactg actgccgcag acgcacaggc
    14521 gtatatggtt tcctatcagc aggcgattca cgccatcggt aaagcgtcta acggtcgtgg
    14581 catctatgaa gggccgggca tttcaatcaa actgtcggcg ctgcatccgc gttatagccg
    14641 cgcccagtat gaccgggtaa tggaagaact ttacccgcgt ctgaaatcac tcaccctgct
    14701 ggcgcgtcag tatgatattg gtattaacat cgatgccgaa gaggccgatc gtctggagat
    14761 ctccctcgat ctgctggaaa aactctgttt cgagccggaa ctggcaggct ggaacggcat
    14821 cggttttgtt attcaggctt atcaaaaacg ctgcccgttg gtgattgatt acctgattga
    14881 tctcgccacc cgtagccgtc gccgtctgat gattcgtctg gtgaaaggcg cgtactggga
    14941 tagtgaaatt aagcgtgcgc agatggacgg ccttgaaggt tatccggttt atacccgcaa
    15001 ggtgtatacc gacgtttctt atctcgcctg tgcgaaaaaa ctgctggcgg tgccgaatct
    15061 aatctacccg cagttcgcga cgcacaacgc ccatacgctg gcagcgattt atcaactggc
    15121 aggtcagaac tactatccgg gtcagtacga gttccagtgc ctgcatggta tgggcgagcc
    15181 actgtatgag caggtcaccg ggaaagttgc cgacggcaaa cttaaccgtc cgtgtcgtat
    15241 ttatgctccg gtcggtaccc atgaaacgct gttggcgtat ctggtgcgtc gcctgctgga
    15301 aaacggtgct aacacctcgt ttgttaaccg tattgccgac acctctttgc cactggatga
    15361 actggtcgcc gatccggtca ctgctgtaga aaaactggcg caacaggaag ggcaaactgg
    15421 attaccgcat ccgaaaattc ccctgccgcg cgatctttac ggtcacgggc gcgacaactc
    15481 ggcagggctg gatctcgcta acgaacaccg tctggcctcg ctctcctctg ccctgctcaa
    15541 tagtgcactg caaaaatggc aggccttgcc aatgctggaa caaccggtag cggcaggtga
    15601 gatgtcgccc gttattaacc ctgcggaacc gaaagatatt gtgggctttg tgcgtgaagc
    15661 cacgccgcgt gaagtagaac aggcgctgga aagtgcggtt aataacgcgc caatctggtt
    15721 tgccacgcct ccggctgaac gcgcggcgat tttgcatcgc gctgccgtgc taatggaaag
    15781 ccagatgcag caactgattg gtattctggt gcgtgaagcc ggaaaaacct tcagtaacgc
    15841 cattgccgaa gtgcgcgaag cggtcgattt tctccactac tacgccggac aggtgcggga
    15901 tgatttcgct aacgaaaccc accgtccatt agggcctgtg gtgtgtatca gtccgtggaa
    15961 cttcccgctg gctattttca ccgggcagat cgctgccgca ctggcggcag gtaacagcgt
    16021 gctggcaaaa ccggcagaac aaacgccgct gattgccgcg caagggatcg ccattttgct
    16081 ggaagcgggt gtaccccctg gcgtggtgca attgctgcca ggtcaggggg aaaccgtggg
    16141 cgcgcaactg acgggtgatg atcgcgtacg cggggtgatg tttaccggtt caaccgaagt
    16201 cgctacgtta ctgcagcgca atatcgccag ccgcctggac gctcagggtc gccctattcc
    16261 gctcatcgct gaaaccggcg gcatgaacgc gatgattgtc gattcttcag cactgaccga
    16321 acaagtcgtc gtggatgtgc tggcctcggc gttcgacagt gcgggtcagc gttgttcggc
    16381 actgcgcgtg ctgtgcctgc aagatgagat tgccgaccac acgttgaaaa tgctgcgcgg
    16441 tgcaatggcc gaatgccgga tgggtaatcc gggtcgcctg accaccgata tcggtccggt
    16501 gattgatagc gaagcgaaag ccaatattga gcgccatatt cagaccatgc gtagcaaagg
    16561 ccgtccggtg ttccaggcgg tgcgggaaaa cagcgaagat gcccgtgaat ggcaaagcgg
    16621 cacctttgtc gccccgacgc tgatcgaact ggatgacttt gccgaattgc aaaaagaggt
    16681 ctttggtccg gtgctgcatg tggtgcgtta caaccgtaac cagctaccag agctgatcga
    16741 gcagattaac gcttccggct atggtctgac gcttggtgtc catacgcgta ttgatgaaac
    16801 catcgcccag gtcactggct cggcccatgt tggtaacctg tatgttaacc gtaatatggt
    16861 gggcgcagtg gtgggtgtgc agccattcgg cggcgaaggg ttgtccggta ccgggccgaa
    16921 agcaggcggt ccgctctatc tctaccgtct gctggcgaat cgcccggaaa gtgcgctggc
    16981 agtgacgctc gcgcgtcagg atgcagagta tccggtcgat gcacagttga aagcagcatt
    17041 gactcagccg ctaaatgcac tgcgggaatg ggcagcaaat cgtccagaat tgcaggcgtt
    17101 atgtacgcaa tatggcgagc tggcgcaggc aggaacacaa cgattgctgc cggggccgac
    17161 gggtgaacgc aacacctgga cgctgctgcc gcgtgagcgc gtgttgtgta ttgccgatga
    17221 tgagcaggat gcgctgactc agctcgccgc cgtgctggcg gtgggcagcc aggtactgtg
    17281 gccggatgac gcgctgcatc gtcagctagt aaaggcattg ccatcggcag tcagcgaacg
    17341 tattcaactg gcgaaagcgg aaaatataac cgctcaaccc tttgatgcgg tgatcttcca
    17401 cggtgattcg gatcagcttc gcgcattgtg tgaagccgtt gctgcgcggg atggcgcaat
    17461 tgtttcggtg cagggttttg cccgtggcga aagcaatatc cttctggaac ggctgtatat
    17521 cgaacgttcg ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat
    17581 cggttaaaca ggtagccgga ggatgttaca acctcctccg gcatctttaa cgtggtcgaa
    17641 tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat acctcatcgc
    17701 gcaacgttgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg
    17761 aagcccaaat cataaaaatc aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga
    17821 cccaaccggc aatcagcgcc gatttctcat caattaatgc cttcaaatcg cccgtcagtt
    17881 catccattaa cagcggcgcg cctgccagca tctccataca gaacaggcgc gaagcctgcg
    17941 gataatcgcg ggagacttcc agcttcagac ggatgtactc ttttatcgcc gccagcggag
    18001 cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc tgccgcagca
    18061 cggcaatata cagcgcctct tttgacggaa agtaatacag cagattggtt tttgaaacac
    18121 ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt
    18181 ccagtgctgc gctaagaatc gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg
    18241 ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt gtagattggc aacaggatag
    18301 caaaaccccc gcccggcctc gaccttctgc actctcatcg cgcacagatg catgttttat
    18361 gtgcaactgt tttgaccgtt tagtccactt tttaccagat attttagaca acccaatgtt
    18421 aacacattaa taacattcac tttttaaaaa ctggcatccg ctttgcaaac aagccattac
    18481 gcggctgaaa aggatatgca ggatgcagcg ccccgtctga ctttcacttt acgagacgaa
    18541 gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg ctggctcatt
    18601 tcgacccacg cgccgcagta catgccgacc tttgaactga ataaagccat tgtgcaaaaa
    18661 gcggagcact accatttcga tttcgccctg tcgatgatca aactgcgtgg ctttggcggc
    18721 aaaactgagt tctgggatca caaccttgag tcgttcacct tgatggcggg gctggcggcc
    18781 gtcacctcgc gcattcagat ttacgccact gctgccacct taacgttacc tccggcaatc
    18841 gtcgcccgta tggccgcaac catcgactcc atctctggcg ggcgttttgg cgtcaacctc
    18901 gtgactggct ggcaaaagcc cgagtatgag cagatgggta tctggcctgg cgatgactat
    18961 ttctcccgtc gttacgacta tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc
    19021 acggggaaaa gcgattttaa aggcgatttt ttcaccatga atgattgtcg cgtcagtccg
    19081 caaccgagtg tccccatgaa agtgatctgc gccgggcaaa gcgacgctgg catggcgttc
    19141 tccgcccagt atgccgattt caacttctgt ttcggcaaag gcgtaaatac gcccacggct
    19201 ttcgccccga ccgctgcgcg gatgaaacag gccgcagagc aaaccgggcg cgacgttggt
    19261 tcttatgtgt tgtttatggt gattgccgat gaaaccgacg atgccgctcg cgccaaatgg
    19321 gaacactaca aagcgggcgc ggatgaagag gcgttaagct ggctaaccga acaaagtcag
    19381 aaagataccc gctccggtac tgataccaac gtccgtcaga tggccgatcc cacttcggcg
    19441 gtaaacatca atatggggac gttagtcggt tcttacgcca gtgtcgcgcg catgttagat
    19501 gaagtcgcaa gcgtgcctgg tgccgaaggc gtgctgttga ccttcgatga ttttctgtcg
    19561 ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc agtgccgcgc ccatctacct
    19621 gcgctgactc aggaggtggc atgatgacaa ccttaaccgc tcgaccggaa gccattacct
    19681 tcgatccgca gcaaagtgcg ctgatcgtgg tggatatgca aaacgcttat gccacgccag
    19741 gcggctactt agatctcgcc gggtttgatg tctcaaccac tcgcccggtc attgccaaca
    19801 ttcaaaccgc cgtgaccgca gcgcgagcgg cagggatgct gatcatctgg tttcaaaatg
    19861 gctgggatga acagtatgtc gaagctggcg gacccggctc accgaatttt cataaatcga
    19921 acgccctgaa aaccatgcgt aagcagccgc agctgcaggg gaaattgctg gcgaaaggct
    19981 cctgggatta tcaactggtg gatgaactgg tgccgcagcc tggcgatatt gtgctgccga
    20041 agccgcgcta cagcggtttc ttcaatacac cgctggacag cattttgcgc agccgtggaa
    20101 tacgccatct ggttttcacc ggtatcgcta ccaacgtctg cgtcgaatcg acgctacgcg
    20161 atggcttttt tctggagtat ttcggcgtgg tgctggaaga cgcaacacac caggcggggc
    20221 cggaatttgc acagaaagcc gcgttgttca atatcgaaac cttttttggc tgggtcagcg
    20281 acgtcgaaac gttctgcgac gcgctttctc ccacatcctt tgctcgtatc gcttaaggag
    20341 tttaacgatg ccaaaatccg taattattcc cgctggcagc agcgcaccgc tggccccctt
    20401 cgttcccggc acgctggctg atggcgtggt gtatgtctcc ggtacgctgg cttttgatca
    20461 acataataac gtgctgtttg ccgatgaccc aaaggcgcaa acccgccacg ttctggaaat
    20521 tatccgcaag gtgatcgaga cggcgggtgg cacgatggcg gatgtgacct tcaacagcat
    20581 ctttattacc gactggaaaa attacgccgc gattaacgaa atctacgccg agttttttcc
    20641 gggtgataaa ccggcgcgat tctgcattca gtgcggactg gtaaaacctg acgcgctggt
    20701 ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc gatgaaactt tcactctcac
    20761 ctccccctta tgctgatgcg cccgtagtgg tgttgatttc gggtcttggc ggtagcggca
    20821 gttactggtt accgcaactg gcggtgctgg agcaggagta tcaggtagtc tgttacgacc
    20881 agcgcggcac cggcaataat cccgacacgc tggcagaaga ttacagtatc gcccagatgg
    20941 cagcggaact gcatcaggcg atggtagccg cagggattga gcattacgcg gtggtcggcc
    21001 atgcgctcgg tgcgctggtg ggaatgcagc tggcgctgga ttatcccgcg tcggtaactg
    21061 tgctgatcag cgttaacggc tggctacgaa taaacgccca tacgcgccgc tgttttcagg
    21121 ttcgcgaacg attactgtat agcggcggcg cgcaggcatg ggtggaagcg cagccgttgt
    21181 tcctctatcc cgccgactgg atggcggccc gcgcacctcg ccttgaggca gaagacgcgc
    21241 tggcactggc gcattttcag ggcaaaaata atttactgcg tcgacttaac gccctcaaac
    21301 gcgctgactt tagtcaccat gcggatcgca tccgctgccc ggtgcaaatc atctgcgcca
    21361 gtgatgatct gctggtgcca tcagcatgtt ccagtgagct tcatgccgcc ctgcccgata
    21421 gccagaaaat ggtgatgcgc tatggcggac acgcctgcaa cgtgaccgac cccgaaacgt
    21481 ttaatgctct tttactcaac gggcttgcca gcctgttaca tcaccgcgaa gccgccctgt
    21541 aaggaattgc tatgaacgaa gccgttagcc caggtgcgct tagcaccctg ttcaccgatg
    21601 cccgcactca caacggctgg cgggagacac ccgtcagcga tgagacgtta cgggagcttt
    21661 atgccctgat gaaatggggg ccgacatcag ctaactgttc tccggcacgg atcgtgttta
    21721 tccgcactgt agaaggaaaa gaacgtctgc gcccggcgct ttccagcggc aatctacaaa
    21781 aaaccctgac cgcgcccgtc accgctatcg ttgcctggga cagtgaattt tatgaacggt
    21841 taccactact gtttccccac ggtgatgccc gcagttggtt tacctccagc ccacaacttg
    21901 ccgaagaaac agcgtttcgc aacagctcca tgcaggcggc ctatctgatc gtcgcttgcc
    21961 gtgcgctggg actggatacc ggcccgatgt cgggttttga ccgtcaatac gttgacgacg
    22021 ctttttttgc aggcagcacg ctgaagagca atctgctgat taatatcggc tatggcgata
    22081 gcagcaaact ttatgcgcgc ctgccacgtc tgtcctttga agaagcctgc gggctgttgt
    22141 aaggagcaaa ctatgaacat agttgatcaa caaacctttc gcgatgcgat gtcctgtatg
    22201 ggcgcggcgg tcaatatcat caccacggac ggtccagccg ggcgcgccgg gttcaccgcc
    22261 agcgccgtct gcagcgtgac cgatacgccg cccacattac tggtgtgcct gaatcgtggg
    22321 gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt gtgtaaatac actgagcgcc
    22381 gggcaggagc cgctttcaaa tctttttggc ggcaaaacgc ccatggaaca ccgctttgcc
    22441 gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac tggaagaggc tctggtttcg
    22501 tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc acgacattct gttttgcgcc
    22561 atcgaagcga ttcatcgtca cgccacaccc tacgggctgg tgtggttcga tcgcagttat
    22621 cacgcgctga tgcgccctgc ttgttaacct cctaaaggag acagctgatg gcaatgttcg
    22681 gttttcctca ctggcagtta aaatcgacct ctacagagag cggcgtggtc gcgccgtatg
    22741 aacgattgcc gtttgctcag acggcagtaa tgggcgttca acacgcggtg gcgatgtttg
    22801 gcgcgacggt gttaatgccg attctgatgg ggctggatcc caatctttcc attttaatgt
    22861 cggggatcgg cacgttactg tttttcttta ttaccggcgg gcgcgtcccc agttatctcg
    22921 gttccagcgc ggcttttgtc ggcgtggtga ttgccgcaac gggctttaac ggtcagggca
    22981 ttaacccgaa tatcagcatt gcgctgggcg ggattatcgc ctgtgggctg gtttataccg
    23041 tcattggcct ggtggtgatg aaaattggta cacgctggat tgaacgacta atgccgccgg
    23101 tggtgacggg tgcggtggtg atggcgattg gcctgaactt agcgcctatc gcagtgaaaa
    23161 gtgtatcggc ttcggccttt gatagctgga tggcggtaat gacggtgctg tgtattgggc
    23221 tggtggcggt gtttacccgt ggcatgatcc agcggttatt gattctcgta ggattaattg
    23281 tcgcctgtct gctgtatggc gtgatgacca acgttttagg gctgggaaaa gccgtcgatt
    23341 ttactctcgt cagccatgct gcctggttcg gtctgccaca tttttccact cctgctttta
    23401 atggtcaggc aatgatgctg attgcgcccg tggcggtgat tctggtggcg gaaaacttag
    23461 gtcatctcaa ggcagtcgcc gggatgaccg gacgcaatat ggacccgtat atggggcggg
    23521 cattcgtagg cgatggtctg gcaacgatgc tttccggctc tgtcggcggt agcggcgtga
    23581 ccacctatgc tgaaaatatc ggtgtgatgg ccgtgaccaa agtctactcg acgctggtgt
    23641 ttgttgctgc tgccgtcatc gccatgctgt tgggcttttc gccgaaattt ggcgcgctga
    23701 tccataccat tcctgcaccg gtgattggtg gtgcttcaat tgttgtgttc gggctgattg
    23761 ccgtcgcagg cgcaaggatc tgggtacaaa accgtgtcga tttaagccag aacggtaatt
    23821 tgattatggt cgcagtaacc ttagtgctgg gcgcgggtga ttttgctctc acgctgggcg
    23881 gttttacgtt gggagggatc ggtacagcaa cctttggcgc gattttactc aatgcgttgt
    23941 taagcagaag gttggttgac gttccaccgc cagaagttgt tcatcaggag ccataaacag
    24001 aaaaccgccg gggtaaccgg cggtgatgac tttttgacga gcttcagaca aaaatgcatt
    24061 cgtcactgat tagtccggct agttgtcact tttaccgtga ctgctcttgc cacctttttt
    24121 acctgcttca gatgcgcgct gcgggtcatt cttgaaatta cccccgctgt gctgtccacc
    24181 ttttttacct gcttctgatg ctctttcgcg gtcttctgca aaattgccgg aaccgcctcg
    24241 atggtttgcc attactgacc tccgtctggg tatagacatc atattgcata gaattaacgc
    24301 ggcgtatggc ggctgccata ttttcgctac gtgggactaa gcttagtgaa ctgacgcggg
    24361 cagacccgcg tttggtaata tcctgcaaca gcttcttagc gagaatagat atcatctcaa
    24421 ataattatcc ataagccgct cttatgatta aaaaaacgcg atgagataaa aatgtgtcat
    24481 tttgcgacaa aattacgtgc ttgtgaaagt tgttataaat caaataagtg gttgtgaaat
    24541 ttgcactctg aaaaggacgt cttatcttta aataagtggt agcgaatcgc tacggaatag
    24601 agataacacg aggagtggtt agaaatggct aaagttctgg tgctttatta ttccatgtac
    24661 ggacatattg aaacgatggc acgcgcagtc gctgagggtg caagcaaagt cgatggcgca
    24721 gaagttgtcg ttaagcgtgt accggaaacc atgccgccgc aattatttga aaaagcaggc
    24781 ggtaaaacgc aaactgcacc ggttgcaacc ccgcaagaac tggccgatta cgacgccatt
    24841 atttttggta cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac
    24901 cagacgggcg gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt
    24961 tccaccggta ctggcggcgg tcaggaacaa actattactt caacctggac gacccttgcg
    25021 catcacggca tggtaattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca
    25081 caggttcgcg gcggtacgcc gtacggcgca accaccatcg caggcggtga cggctcacgc
    25141 cagccaagcc aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg
    25201 gcagttaaac ttaacggcta atcttcaaca ggaggatacg catgccaact caagaagcga
    25261 aagctcatca cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg
    25321 ccattttcga agtcgccggg tacgacgaaa aaatggcgga aaagatttgg gaagaaggta
    25381 gcgatgaagt gttagtcaaa gcctttgcta agaccgataa agattcgctt ttttggggcg
    25441 aacagaccat cgaacgtaaa aacgtttaac cagcgactcc cccacttctc gcgggggagt
    25501 tttctgttat ttcaccgcct cattcaacac gctatcaaac ttatccattg ggcagaaacc
    25561 gttggcatct atcgggcaac cgcttaattc cagcgtcaca cgctgagcag gtgcctgcag
    25621 ggttaacgca tcggcattac gtaactgttc cgcactctga tacacatatt caattttcat
    25681 caaatcgcga ttggctttgc tgtcatgcca acgctggaaa acgattttgc cgccaatcgg
    25741 cgtgcgttcg ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga
    25801 ggcaatgttg gagtcgtgcc caaccaacac tgtaattttc ggtgcgctgg tgcgatcggt
    25861 gaccagagct ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg
    25921 tgaggtaaac aggctgtcct ggtagccgtt tttaagcttc gacaacacct tccactgctg
    25981 gtcagatttg atttctcccc aggccacctg atccatcgga aaaccttcgt aatattgcaa
    26041 agtaaacgca tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg
    26101 ttgatacttg gcgctaaagg tatttttgcc atccaccagc gaacactgtt gtttctcttt
    26161 acaggcaggg gaatctttat agttaacgat tttttccagt agttggtagc tgtcggtaag
    26221 ctggagtttg ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga
    26281 atcatcggtg atcaccgggt taaaggttgg gtccatagtg cccatttttt cctggtgatg
    26341 cacaggaata tcacaccccg ggaatgcgcc ggtaataaag aactgtgcgg tcgcaacggt
    26401 acgttgcaga ctattggcat aggcataaac ggtgtccggc ggcgggcatt cccccgattt
    26461 caccatcccc tgctgtgcca gccattcacg catgtaatgg cccatataca cttcgagcac
    26521 gccacctttg gtggtgagtt gcccaccggg gacgtcccat tcgggccatt tattcggcgt
    26581 agactgctcc agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat
    26641 catgagcact tgctgtagct gatagccttc cggtacggtt tgtgcctgag cgtttgaagc
    26701 gagtaaaact atccctgcca cagctgcggc gattagcgtt ttgttcattc ctggcacctc
    26761 ttttgttatc aatcactaaa gagtgtgaca gaaatatgac ttttttccgg gaacaggttc
    26821 gcaaaatggc ttgttgaacg cctgtttttc tatatacatc ttcaaaaaca ggcaaggttt
    26881 gacgcaaact atgcctgatt agcccctgcc tcgcgtacgc cgctgataat tgcacctgtg
    26941 gctaaacaga atatctgcgg cgttaccaat acagagacaa tatttaccaa taaaaaagtg
    27001 tgagactaat aacaatggtt tttagcatat gcctttgcca ttaaattgca taacgattta
    27061 aatagtcgag ttgcgcattc atttccttat tttttaactt ataagcctga gcaaaaagtt
    27121 caggagggag gtaaacatca taatgattgc cagcgtttat taaataacca tactcaaggt
    27181 cgtcatctgt tgaaaaggat tcattcccca aggagattga t
//
LOCUS       DAZFRN010000057        27028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFRN010000057 DAZFRN010000000
VERSION     DAZFRN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.057"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..174)
                     /gene="yhdU"
                     /locus_tag="ACHKUB_002331"
     CDS             complement(<1..174)
                     /gene="yhdU"
                     /locus_tag="ACHKUB_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO6655343.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="ACHKUB_002332"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="ACHKUB_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6655344.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="ACHKUB_002333"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="ACHKUB_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO6655345.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="ACHKUB_002334"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="ACHKUB_002334"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO6655346.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="ACHKUB_002335"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="ACHKUB_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO6655347.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="ACHKUB_002336"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="ACHKUB_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO6655348.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /gene="yhdT"
                     /locus_tag="ACHKUB_002337"
     CDS             complement(5178..5420)
                     /gene="yhdT"
                     /locus_tag="ACHKUB_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO6655349.1"
                     /translation="MDTRFVQAHKEAHWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="ACHKUB_002338"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="ACHKUB_002338"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO6655350.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="ACHKUB_002339"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="ACHKUB_002339"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO6655351.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /gene="yhdH"
                     /locus_tag="ACHKUB_002340"
     CDS             complement(8337..9311)
                     /gene="yhdH"
                     /locus_tag="ACHKUB_002340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO6655352.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="ACHKUB_002341"
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="ACHKUB_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO6655353.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11708..12751
                     /gene="mreB"
                     /locus_tag="ACHKUB_002342"
     CDS             11708..12751
                     /gene="mreB"
                     /locus_tag="ACHKUB_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO6655354.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="ACHKUB_002343"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="ACHKUB_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO6655355.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVINKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="ACHKUB_002344"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="ACHKUB_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO6655356.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="ACHKUB_002345"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="ACHKUB_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO6655357.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="ACHKUB_002346"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="ACHKUB_002346"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO6655358.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /gene="yhdP"
                     /locus_tag="ACHKUB_002347"
     CDS             16537..20337
                     /gene="yhdP"
                     /locus_tag="ACHKUB_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO6655359.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGAVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="ACHKUB_002348"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="ACHKUB_002348"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO6655360.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="ACHKUB_002349"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="ACHKUB_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO6655361.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /gene="aaeX"
                     /locus_tag="ACHKUB_002350"
     CDS             23178..23381
                     /gene="aaeX"
                     /locus_tag="ACHKUB_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO6655362.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="ACHKUB_002351"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="ACHKUB_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO6655363.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="ACHKUB_002352"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="ACHKUB_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO6655364.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /gene="yhcO"
                     /locus_tag="ACHKUB_002353"
     CDS             26386..26658
                     /gene="yhcO"
                     /locus_tag="ACHKUB_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO6655365.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="ACHKUB_002354"
     CDS             complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="ACHKUB_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO6655366.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6430 a         6794 c         7221 g         6583 t
ORIGIN      
        1 tctgtcctga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attgcggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcagagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgcccgct ggagacggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 tccggttttt gcgtcgggtg gttctcatat tcatccatta aataacgcac gcgcggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tcgatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaga ccatcaaaat ttttaccgat gttatatggt
     1021 gggtcggcaa agatcagatc gacactttcg gcggggatct ttttaagttc ggcaagcgca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cccatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg atcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagctg tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac gccattacgt
     3121 tcggcgttgt cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagtgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcgatttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcaaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacggt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca actgggcatt gatcgtcgac atgatcgcag
     4201 ccataggtgc agccaggaag attccggcag caaacggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ccaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac tgcaggaagc tgaatcatcg ccagcaatac ccagcccagg ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaacag agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcggctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agtgcgcctc tttatgggcc
     5401 tgaacaaaac gagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcgag caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgcactcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcac aacgttcgcc gatgtagcga
     6121 cgcagttccg gtgtaattcc cggtgctggt gcttcttcga ccactttctg gtgacgacgc
     6181 tgcatagagc agtcacgttc cgccagatag atagcgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga cgtggcgagg attttccagg tatttctcca tgtaaaccat atcgttattg
     6301 aatgcggctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacac gcataccacg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 cctatgcgtt tagcaatggc acggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 cctggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgccgacac tttgtcaccc
     6541 atcaggcgaa tagtgtctgc tttcgggcca atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgcttat gattgccggg atgttcagat aactttttac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggagcg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctgatgcgt actgactctt cgccttcgga gatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgtgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgtaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccacaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 cgactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggga ccagaaatca gtaataggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcatatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accctggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tggttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 agcactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cgactggcac ctaaactttt caggtattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcaaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt caagtgtctg taccgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc aattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagca cattaaggcc acaaattacc
     9661 gaattaatga tgtcggcaga tatcgttcgt gtagacctgc tccatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggct ccagcgatct gtttcgcgaa
     9781 ctgagtgttc cgttgataaa gcatccgggg atgtcgctgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc tttaatgact accgcgccgc tcacgggggc gattggcttt
     9901 atcattgtta tgctcttcct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctag atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcccag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacttg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catcgccggt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgcgc ctggcgtagt ggtcaggata ccgagcaggt aatggaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggcggca atagctgggc tatttacgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgcccgcg ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt cagaaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccaag tttacgccac cggtgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatctgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gaaccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa atgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcgtatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcaat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga ttcaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accgacgaca
    13801 ggaaatgcag ctactgcgcc tgctgcgccc acacagcctg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgttggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 tcatcttgtt gtattggatc ctggccttgc ctcatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgacgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgcag
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acaggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcgcgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcgc aggatctccc ggtgctgggt gcggatacta tcgttatcct
    14641 gaatggagaa gtgctggaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcacat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgatatcat gccgcacacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tgtttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg tattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gtaccgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 aacgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgatttcgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgctgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggggcgtttg tcggtcatcg caatctggac gacaccattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgcaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgacgcgtgc
    16081 tgcactcgct ggagcaggca ttgagcaaag accgggtgaa aactagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagctgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggtaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg acatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcttg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tggtaatgat
    16921 agcctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcagc gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg gacagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttggcggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcca gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttacttaagt
    18121 ggcgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactcg aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcaccctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccag cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagatga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaactcaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacgtta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaaactc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccggag
    19681 aatgaacgta cctcgctgaa aggaaaactg cgcgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca accagatgag gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgttg atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggggt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaaggggc ggtaaatctg
    20101 gtacgtcgcg acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtatttgc agccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gacgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cgtaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggtgtgcgtg
    20701 caatcagtgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgcccgc gaagcggaca
    20941 agcgcgtaca agaagtgact gccagcctca gcggcgttta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctggcg gcggatgttc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttggtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgc ggcacttcag tatttagtgg gcaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcag gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatattacat ccggcaaatt cgttttctcc acttcagaag
    21721 cgtatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcgacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgc
    21901 tgaaagttga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tcaccgcgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattgacg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ctgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gcgcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtggatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    24961 gacgcaccac ggcgctacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtcaa agtagagtca gccgaacgtc
    25381 atcatgcgat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtagtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tacgggacgc
    25561 tggccgcgct gccgttaggg ctgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt tttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcgc tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc ttgtgcggga taaatcgcgc gacaggaccg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactt gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatacc
    26401 tttgattttg atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccttt
    26461 ggtctggcta aagataaggt acgcgatctc gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaag cccccgaacc gggggcaata tcgtcggaca
    26701 agacgatgag ggtttatttg tacagttcag ccgtagcgtg ccaggtgtca ccgctacgag
    26761 cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc ttttttgttc agcatttcac
    26821 gcatatccat tggcgaagac gccacaccac ttacggatac ggtcccgatt gcttcacgat
    26881 tttgtgcttg tgcagcatca atggagtcgg cagcgaatgc accgaaagag agaacagaaa
    26941 gtacgcttaa tgcagctaca gtggttttga ttttcatgat ttttacctcg acataatctt
    27001 ttagctgggc ctttgtttcg tgacccat
//
LOCUS       DAZFRN010000058        26712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFRN010000058 DAZFRN010000000
VERSION     DAZFRN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.058"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(219..1259)
                     /locus_tag="ACHKUB_002355"
     CDS             complement(219..1259)
                     /locus_tag="ACHKUB_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6655367.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(1387..3348)
                     /gene="topB"
                     /locus_tag="ACHKUB_002356"
     CDS             complement(1387..3348)
                     /gene="topB"
                     /locus_tag="ACHKUB_002356"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO6655368.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(3353..4396)
                     /gene="selD"
                     /locus_tag="ACHKUB_002357"
     CDS             complement(3353..4396)
                     /gene="selD"
                     /locus_tag="ACHKUB_002357"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO6655369.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(4513..5064)
                     /locus_tag="ACHKUB_002358"
     CDS             complement(4513..5064)
                     /locus_tag="ACHKUB_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO6655370.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            5225..7081
                     /gene="sppA"
                     /locus_tag="ACHKUB_002359"
     CDS             5225..7081
                     /gene="sppA"
                     /locus_tag="ACHKUB_002359"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO6655371.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAEQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGKVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            7248..8264
                     /gene="ansA"
                     /locus_tag="ACHKUB_002360"
     CDS             7248..8264
                     /gene="ansA"
                     /locus_tag="ACHKUB_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO6655372.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            8275..8916
                     /gene="pncA"
                     /locus_tag="ACHKUB_002361"
     CDS             8275..8916
                     /gene="pncA"
                     /locus_tag="ACHKUB_002361"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO6655373.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(9009..10367)
                     /locus_tag="ACHKUB_002362"
     CDS             complement(9009..10367)
                     /locus_tag="ACHKUB_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6655374.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(10484..11242)
                     /locus_tag="ACHKUB_002363"
     CDS             complement(10484..11242)
                     /locus_tag="ACHKUB_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6655375.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSGLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     IRRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(11379..12359)
                     /gene="ydjG"
                     /locus_tag="ACHKUB_002364"
     CDS             complement(11379..12359)
                     /gene="ydjG"
                     /locus_tag="ACHKUB_002364"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO6655376.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVAASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKSE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(12369..13316)
                     /locus_tag="ACHKUB_002365"
     CDS             complement(12369..13316)
                     /locus_tag="ACHKUB_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6655377.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFIFDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(13321..14157)
                     /locus_tag="ACHKUB_002366"
     CDS             complement(13321..14157)
                     /locus_tag="ACHKUB_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO6655378.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(14178..15221)
                     /locus_tag="ACHKUB_002367"
     CDS             complement(14178..15221)
                     /locus_tag="ACHKUB_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO6655379.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGTKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(15238..16617)
                     /locus_tag="ACHKUB_002368"
     CDS             complement(15238..16617)
                     /locus_tag="ACHKUB_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6655380.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(16644..17720)
                     /locus_tag="ACHKUB_002369"
     CDS             complement(16644..17720)
                     /locus_tag="ACHKUB_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6655381.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(18090..18362)
                     /locus_tag="ACHKUB_002370"
     CDS             complement(18090..18362)
                     /locus_tag="ACHKUB_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO6655382.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(18404..18817)
                     /gene="msrB"
                     /locus_tag="ACHKUB_002371"
     CDS             complement(18404..18817)
                     /gene="msrB"
                     /locus_tag="ACHKUB_002371"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO6655383.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            19159..20154
                     /gene="gapA"
                     /locus_tag="ACHKUB_002372"
     CDS             19159..20154
                     /gene="gapA"
                     /locus_tag="ACHKUB_002372"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6655384.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            20238..21122
                     /locus_tag="ACHKUB_002373"
     CDS             20238..21122
                     /locus_tag="ACHKUB_002373"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO6655385.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(21173..22027)
                     /gene="yeaE"
                     /locus_tag="ACHKUB_002374"
     CDS             complement(21173..22027)
                     /gene="yeaE"
                     /locus_tag="ACHKUB_002374"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO6655386.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(22117..22863)
                     /gene="mipA"
                     /locus_tag="ACHKUB_002375"
     CDS             complement(22117..22863)
                     /gene="mipA"
                     /locus_tag="ACHKUB_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO6655387.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            23299..25233
                     /gene="yeaG"
                     /locus_tag="ACHKUB_002376"
     CDS             23299..25233
                     /gene="yeaG"
                     /locus_tag="ACHKUB_002376"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO6655388.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            25346..26629
                     /locus_tag="ACHKUB_002377"
     CDS             25346..26629
                     /locus_tag="ACHKUB_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO6655389.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         6905 a         6866 c         6197 g         6744 t
ORIGIN      
        1 atgcgtcacg gcataatccg gtagcggcgt aaaaatcgcg gaagggatga aaaaaacagc
       61 gcctgacggc gcagtgtctg gcatgcctgc aatccgggaa accggaccag gaaaaaactt
      121 gcaggccata acagtatcta caaatgggca caattcattg cagttacgct ctaatgtagg
      181 ccgggcaagc gcagcgcccc cggcaaaatt tcaggcgttt atgagtattt aacggatgat
      241 gctccccacg gaacatttct tatgggccaa cggcatttct tactgtagtg ctcccaaaac
      301 tgcttgtcgt aacgataaca cgcttcaagt tcagcatccg ttaactttct gcgataacag
      361 cagatatgcc agtaaagaaa ccccattaga ctattttttt gataatcttc ttcgctttcg
      421 aacaactcgt gcgcctttcg agaagcaagc attatataat gccaggccag ttcttcttca
      481 attgtcccgt tttgaaaagc tgtgcttgat atcgaggtca tccatgataa ttccgccgcc
      541 catattagct tcgccgagga tttaccggag cgattattag cgcaatcaga gatatagtct
      601 gagggaaaaa cagcaaattt attcaacaag gcgataacct gctctggggc ttcctccatg
      661 tttgctttaa aggtattggc tccatggtcg ccagaaagaa aatgctccat taaggcacaa
      721 taactttcgc tatcttcgat accccattga tcctctaaag actcgcgtct tttacttatg
      781 atatcgatcg agtcaaaagg aagcacatga tattggaagg tatctttgcc aggttcaggc
      841 tttcgcggcc agaactccag cgtttcagac cattgcttat gatagaatcg ataaggtgcg
      901 atcaattgta gcgcctgtaa cttctcgata ctgagcggct caataccttt agcctgataa
      961 taatgcagtt gttctttttt tgctttaaaa ccggcccgaa caataagccc catcataatt
     1021 aatagataaa gaaaagagca tcccgcggta atcaggcctc tttcattcaa accgttggat
     1081 gttatcgctg cgaacacaaa cattacagcg acaacacatg ttaaataaaa cccccactta
     1141 caaagcagca tggccttatt ttctttaatc atccgttcaa aattactatt aaatatttcc
     1201 cagccattaa aagaatactt ctcgctccca ggatggtttt gtaataaaac ttttttcatc
     1261 acattccctg ttattacata ctttagaggc gataatttta tccatgcaaa aaaaatatcc
     1321 aacgaatttt cttgatccgc tggatatttc agaatatgac tcgaatagca cgaaagattc
     1381 actcgcttac gctatcgccc cgcttccgac ttcatctgct ggcggacttt ttttcgcact
     1441 acgtttacgc ggtgcagcct ttttcttatc agcactgcca ccactgcccg gagccacaat
     1501 gccgcgaaac tgccgcaccg gcgtacgttt ggcttgatca ataagctgat atagcgtccc
     1561 caccagcggc tgcataaagt cctgatagcg acactgcttt tcgctgattt gcgtcagcac
     1621 cgattcccag tgcgcggtca tgtccggtcg cgtcgccatt tctggcagcg aatggaataa
     1681 cgcttttccg gcgtcggtag agtggatata gcgccctttt ttggtcagaa aaccacgctt
     1741 gaacagcagt tcaataatcc cggcacgggt tgcttctgtc cccagaccat cggtcgcacg
     1801 aaggatcttt ttcagatctt tgtcctgcac aaagcgcgcg atcccggtca tcgccgaaag
     1861 cagtgttgca tcggtaaaat ggcgcggcgg ctgggtttgc cgctctacca cttcaccttt
     1921 ttcacacagc aactcatcgc ctttcgccac cacaggcaat ggcgtaccgt cgttttcttc
     1981 atcgcgctct ttgctgccta acagcgtgcg ccagcctgct tcagcaagaa aacgggcttt
     2041 agcgacaaat ttgcctttgg caatgtccag ttcgataaca cacttgcgga acaccgcatc
     2101 cgggcagaac tgcatcaggt attgacgggc aatcaggtta tagaccttcg cttcgttctc
     2161 cgtcaggttg atcgcagaac tccgtgcggt cgggatgatg gcgtggtgcg catcgacctt
     2221 tttgtcatcc caacagcggt tgcgtatatc tggatctacc actgactgcg gcaacagatc
     2281 cggtgcatga acgctgatgg cattcatcac cgcgtggcgt ccggcaaaat gttcttctgg
     2341 caaatagcga caatcggaac gcggataagt gattagcttg tgcgtttcgt acagcttctg
     2401 gcagatatca agcacgttct gcgcgctcag accgaagcgt tttgccgctt caatctgcaa
     2461 cgccgaaagc gaaaacggca gcggcgcgga ttctgattcc cgtttatcgt tatagctggt
     2521 gacaatagcc ggttgaccac taatgcggtt aaccacatgc tccgccagtg gacgatgtaa
     2581 caagcgccct tcttcatcct ggtacggttc acacgcttcg ctcggttgcc agatagcggt
     2641 aaaccgctca tcggcaggtg tcacgatatg cgctttgact tcaaagaagt ctttcgccac
     2701 gaagttttca atctcttcat cgcggcgcac caccagcccg agcacgggtg tctgcacgcg
     2761 tcccacggaa agtacgccct gataaccggc attgcgaccg agaatggtat acgcacgggt
     2821 catattgatg ccgtacagcc agtcggcacg cgctcgcgcc agcgcagaaa cgcacagcgg
     2881 tacaaactca ctgttggaac gaagacggtc gatcgcccgc tcaaccgcct gcgggttcag
     2941 gtcgtttatc aagcaacgct gcacctgctg gcgcttttcc ggtgccagtt gtagatagtc
     3001 cagcacttca tccaccagca gttgcccttc gcgatccggg tccccggcgt gaacgatttc
     3061 gctggcttca tgcaggaacc gtttgatgac gttaagttgt ttggtcacgg agggtcgggg
     3121 ctgtaattgc cacttttccg ggacaatcgg caaatccgca agattccagc gcgcatagcg
     3181 gctgtcgtag gcgtctggct gcgcctgctc aagcaggtga ccgatacacc aggtcaccac
     3241 ctgaccatta ccgcactcga taaagccatc gcctttacgg tgcggtttgg gcaggacatc
     3301 agcaatggcg cgcgccagac tcggtttttc ggcaataaac aaccgcattg aattaacgga
     3361 tctcaaccat ggcacgaccg ccgcgcgctg gcaccagttc gccaatcgcc gtcagttcaa
     3421 tgccaaactc ggcggctgta gctttaacct cattttctgc ttccggcatg accgccagca
     3481 gcaaaccgcc agaagtttgc ggatcgcaca gcagatcgcg cacttcacgc ggcatttcac
     3541 ccatcagatg accgtagctg gcaaagttac gttcggtgcc gcctggcact gcgcccaact
     3601 taatgtactc ttcaaccccc ggcaatttcg ggatcgcgtc atagtcaacg cgcgcctgca
     3661 cacctgcgcc ctgacacatt tcgctcaaat ggcccagcag accaaagccc gtaacgtcgg
     3721 tcatcgcttt tacgccttcg atgttggcaa aggacgcgcc tgcgatattc atccggcaca
     3781 tcacttctgt cgccagtccc tgatgttctg gtttcagcag tgattttttc tcagccgtgg
     3841 taagaacgcc gatccccagc ggtttcgtca ggaacagttt gcatccggct tgtgcggtac
     3901 tgtttttctt cacccgctcg gtcggtacga tccccgttac cgccagacca aaaatcggct
     3961 ccggcgcatc gatggagtga ccgcccgcca gcgcaatacc cgcctgacgg catgcatagc
     4021 gtccaccttc ggttacttcg cgggcaattt ccggggaaag tttgttaatc ggccagccga
     4081 ggatcgcaat cgccataatc ggtttgcccc ccatcgcgaa gatatcgctg atggcgttag
     4141 tcgccgcaat gcggccaaaa tcgaaaggat tatcaacgat cggcataaag aagtcggtgg
     4201 tactgataac gctggtgcca ttgcccagat cgtacaccgc cgcatcgtcg cgggtctcat
     4261 tacccacaag caaattcgga tcaacaaact tcgcctgctc actgtgcagg atggtttcca
     4321 acacttttgg ggaaatttta cagccgcaac cagctccgtg gctgtattgg gtcaaacgaa
     4381 tcgagttctc gctcatggac atctcctgtc aatgcaatcc gggtatggta accctcattc
     4441 cgtgaagtga taagtgagag tgtctgaatt cctgcgcctt tgctcacaat ccagacagtt
     4501 tcgcgacaat tatcagaaat aagttacaaa cggcgtcggg tccgggacgt taatcgacgt
     4561 agatgctttc aactgcggcg tgccgaggta gagaaaaccg acaattttat cctgctcacg
     4621 gcaaccgaat gcttcacgca ctaccggact ttcagttaat gcgccactgc gccagatgcc
     4681 gccaaatcct tgggcaacgg ctgccatttg catcgccatg accgcgcatc cggcagacat
     4741 ttcctgttcc catcgcggga ctttatgatt ttcttcgcat ttcgccacca ccgtgatgat
     4801 gagcggtgcg cggaacggcg cattacgggc tttgtcgata gctttgtcat cactaccggc
     4861 agcaatcgcc ccctgttcca gtacggcgct gaaacgctcg cgcccttccc cttcaatcac
     4921 aaaaaaatgc cacggttgca tggacttatg gtccggcgca cgcatacccg cacgcaggat
     4981 gttttgcagt tgttcacccg ttggcgcggg ttcagccagg cgggaggcgc tacggcgatt
     5041 gatcaatagt tcgagtgcat ccatttgatt aactcctgtc gtgatattta ttcacaaaat
     5101 taacacgaga gtggattttg ttacagcaca gtccgcaatt cctgctgaca agtgccggtt
     5161 gggtcattac gataaccaca tctattgcgc ctgtgacagg tgtgacctta agttgggaga
     5221 atacatgcga accctttggc gatttattgc cggatttttt aaatggacgt ggcgtctgct
     5281 gaatttcgtc cgtgaaatgg tacttaacct gttctttatt ttcctcgtac tggttggtgt
     5341 ggggatctgg atgcaggtca gtggtggtga ttcgaaagaa acggccagtc gtggcgcact
     5401 gctgctggac atttctggtg tgatcgtcga taaacccgac agttctcagc ggtttagtaa
     5461 attaagccgc cagctgcttg gtgccagttc cgatcgtctg caggaaaact cactgtttga
     5521 tatcgtcaac accattcgcc aggcgaagga cgaccgcaat atcaccggta tcgtgatgga
     5581 tctgaaaaac ttcgcaggcg gcgaccagcc gtctatgcag tacatcggca aagccctgaa
     5641 agagtttcgt gacagcggga aaccggttta tgccgttggc gagaactaca gccaggggca
     5701 atattatctc gccagtttcg ccaataaaat ttggctgtca ccgcaaggcg tggttgatct
     5761 gcacggtttt gctaccaacg gtctgtacta caaatcgttg ctggataagc tgaaagtttc
     5821 cacccatgtg ttccgcgtgg gtacgtataa atctgccgtt gaaccgttta ttcgtgatga
     5881 tatgtcaccg gcagcccgcg aagctgacag ccgctggatt ggtgagctgt ggcaaaacta
     5941 tctgaatact gttgccgcta accggcagat ccctgctgag caggtattcc ctggcgcgca
     6001 agggttgctt gagggtttaa ccaaaaccgg tggcgatacc gcgaaatatg cactggaaaa
     6061 caagctggtc gatgcactgg catcgagtgc ggaaatcgaa aaagcactga ccaaagaatt
     6121 cggctggagt aagactgata aaaattatcg cgccatcagt tattacgatt acgcattgaa
     6181 aacgccggca gataccggtg acagcatcgg tgtcgtcttt gctaatggcg caattatgga
     6241 tggcgaggaa actcagggga aagttggcgg tgataccact gcggcacaaa tccgcgacgc
     6301 tcgccttgac ccgaaagtga aagcgattgt cctgcgtgtt aatagcccag gcggcagcgt
     6361 taccgcgtct gaagtgattc gcgctgaact ggcagcagcc cgggcagcgg gtaagcctgt
     6421 ggttgtatcg atgggcggca tggcggcatc tggtggttac tggatttcca cgccagctaa
     6481 ttacattgtg gctaacccca gcaccctgac cggttctatc ggtatcttcg gcgtgatcac
     6541 caccgtagaa aatagtctgg attcgattgg tgttcatacc gatggtgtct caacttcacc
     6601 gctggcagat gtttctatca ccagggcact gccgccggaa gcacagcaga tgatgcagtt
     6661 aagcattgag aatggctata aacgctttat cacgctggtt gctgatgcgc gtcattcgac
     6721 gccggagcaa attgataaaa tcgcccaggg ccacgtctgg accggtcagg atgcaaaagc
     6781 taacgggctg gtcgatagtc tcggggattt cgatgatgcg gttgccaaag cagcagagct
     6841 ggcaaaagtg aaacagtggc atctggaata ctacgttgat gaaccgacct tcttcgacaa
     6901 agtgatggac aacatgtctg gttctgtccg ggcaatgttg ccagatgcgt tccaggccat
     6961 gttacctgca ccgcttgcct cggtagcctc taccgttaaa agtgaaagcg acaagctggc
     7021 cgcgtttaac gacccacaaa accgttatgc gttttgcctg acctgcgcca acgtgcgtta
     7081 agtcttgtac tgagtggccg acagatcgtc ggccacatta ttttttacct cgacgaatcc
     7141 tcttcccgct gtctcgcccc atatttcctt atccgcatag tatcaggtgc gctcctcctg
     7201 cctcacgtat atacttttgc tctttcgata tcattcatat caatatcatg caaaagaaat
     7261 caatttacgt cgcctacacg ggcgggacca tcgggatgca gcgttccgag cagggttata
     7321 ttccggtgtc aggtcatcta caacgccaac tggcgctgat gccggaattc catcgcccgg
     7381 agatgccaga tttcaccatt catgaatata cgccgttgat ggattcttct gatatgacgc
     7441 cggaagactg gcagcatatt gctgaagata ttaaagcgca ctatgacgac tatgatggtt
     7501 ttgtcattct acacggtacc gacacgatgg cgtataccgc ctctgcgctg tcgttcatgc
     7561 tcgagaatct cggtaaaccg gtcattgtga cagggtcaca aatcccgctg gctgagttac
     7621 gctctgacgg acaaattaat ctgctgaatg cgttgtacgt tgcggcgaat tatccgatca
     7681 acgaagtaac gctctttttc aataaccgat tgtatcgcgg caaccgcact accaaagccc
     7741 atgccgatgg ttttgatgcg tttgcctctc caaaccttcc tccgttactg gaagctggta
     7801 tccatattcg tcgtttgaat acgccacccg ccccgcacgg tgaaggggaa ttaatcgttc
     7861 atccaatcac gccacaacca attggcgtag tgacgattta tccagggatt tctgctgacg
     7921 tcgtgcgcaa ttttctgcgc caaccggtga aagcattgat tctgcgctcc tatggcgtgg
     7981 gtaatgcgcc acaaaacaaa gccttcctgc aggaattaca agaagccagc gatcgcggta
     8041 ttgtggtggt caacctgaca caatgtatgt ccggtaaagt gaatatgggt ggttatgcca
     8101 ccggtaacgc cctcgcccat gccggcgtta ttggcggtgc agatatgact gtagaagcca
     8161 cgctaaccaa actgcattac ctgctgagcc aggaactgga tactgaaacc attcgcaagg
     8221 ccatgagcca aaacctgcgc ggcgaactga cgccggatga ttaaggagac tgcaatgccc
     8281 catcgcgccc tgttactggt cgatttacaa aatgatttct gtgctggtgg cgcgctcgcc
     8341 gtgccggaag gtgacagtac ggtagatgtc gctaaccgcc tgattgactg gtgccagtcg
     8401 cgcggtgaag cggttatcgc cagtcaggac tggcacccgg cgaatcacgg cagttttgcc
     8461 agtcagcacg gtgtagagcc ttatacgcca ggccaactcg acggtttgcc acaaaccttc
     8521 tggccagatc actgtgtgca gaacagtgaa ggcgcacaat tacatccgtt actgaaccaa
     8581 aaagcgatcg cagcggtgtt ccataaaggc gaaaatcctt tagttgacag ttacagtgcc
     8641 ttttttgata acggccgtcg gcagaaaacc tctctcgatg actggttacg cgatcatgaa
     8701 atcgatgaat tgatcgttat gggcctggct actgactatt gcgtgaagtt taccgtgctg
     8761 gacgcgttac agttaggtta taaggtaaac gtgattaccg atggttgtcg tggcgtgaat
     8821 atccagcccc aggacagtgc gcacgcgttt atggagatgt cagcagctgg ggcaacgcta
     8881 tatacgctgg cagactggga agagacgcag gggtaatttt acgctggcct acaattctgt
     8941 actggcattg taggccaaat aaaacacgtc agtggcacat ctggcaattg atgccatcaa
     9001 cgaaagattt aattcacctc agaaatctct tccaacgaca ccttccgcgt ttcgatgcca
     9061 aaaatggaga gaaccagcgc acaaagcaat aacatgacac caagtaccat aaacaccgtg
     9121 atcgacccat aatgtgttaa taatgccgca acgccatagg gcgtgaaaac tgcgacgatc
     9181 cgtccgacgg cattaacgaa acccgaaccg cgcaggcgta aatgcgttgg ccaaagctcc
     9241 gggatataaa ccgccgacgc gaagcaaaca tacatgtata aaaagaagat catcaccagg
     9301 ccatagatta aaatcgccca ctctgtagtc tgaattgaat agatatagcc taacacggca
     9361 ataataataa gtaaggtgga gccaaataac cgacgaggaa aatgatcaat aattaatgcc
     9421 gcaataaata ttcctaccgg agcgccaatc ataataacag cggtcattaa tattgattta
     9481 tcgacatcaa tgccggagtt aacaaaaatg gtcggtatcc atacggtgat ggtataaagc
     9541 gagatgttca ttgcaattaa aacagtaatc gcgactaatg tacgtcgtaa catttcacct
     9601 ttaaacagga gccagaaagt acccttgatt actttaacct tgctgttgct ctgatacgaa
     9661 gtaagcgggg gtaaacgaat actcttctct ctttcaattt gctgctctac ttcacgaagt
     9721 tggctttctg caccggcgat ttgccctttc cctgccagcc atcgtggcga ctcgataaag
     9781 tatttacctg agagaaacca ggctaacagt atgccaatac cacccagcag aaacattatt
     9841 cgccaactaa aaaaagcgat aaccaccacg cctatcgccg cagacagcat gggcgaccag
     9901 ttaccaacaa atgagagccg cgcggaccat tttccacgca ccgtcgcggg gataaactcc
     9961 gtaaatgagg catagccaac cataatcagc gcccccattc ctgttcccat caggaagcga
    10021 aagaagatga gccagtacat atcagggaca aaagcggccc ctgttgcagc aataccgacg
    10081 atgagaagat ttatgcgaaa cgccctgcgc cgcccaaagt agtcaccaat aaacccacca
    10141 gtaagtgagc cgatgaaata accaaacatt aatgccgagg taaaggcggc attgaggaaa
    10201 ttatttgacc atccattgct taccagcttt gctaagacga cattaccgga gtaactcaaa
    10261 aaccctgtta ataacagact aaagcttata ataccaaata tacgataatg aaaccgggcc
    10321 aaaggcaagc ggtccagtct tgcgccaatt tgatcatatt gttccattag gtgcctcgga
    10381 tttgctaaaa aggtagcaat tgcggcaaat tcacttgccg catctttatc atcattttat
    10441 ttcaaggcgc aggaacaaat aatattgaca acgtgtgctt aaattaccac accagctgga
    10501 tattattgtc tttgcaaaat gctatccact ctgcacccgg tgatttatca gttattatgt
    10561 aattaatatg actaaaatca gctaactgga caaaagcttt gcgatcaaac ttagagtgat
    10621 caaccaataa cgcaacttct gtcgcctgac ggatcattgt ctttttgatt tcagcttccg
    10681 cttcattaga gtccagcgcg ccgctgttaa tatcaagacc tttacaactc attaccatga
    10741 tatcgacatg atagcgcctg atgatctctt tagtaattct tccttgcagt gaaagtgtat
    10801 ttttgtttag ttccccaccc gttgaaacga ctttaatttc tgactgagcc agaacatgaa
    10861 tcgcttccgc cgagttggtt agcaacgtca ggccactacg gtcctgtaac aatttgagca
    10921 attccattac ggtactactg gaatcggctg ccatggtggt tttattgtcg ataaagggta
    10981 gtgccttgcg tgcaataagc tgcttctctt cataaaacga tgaagcgcgc ttataaaaat
    11041 ggatattctc cgtcaacatc gctgtattta aaacagcacc accataggtt ctggtcaaaa
    11101 agccttcatc ttccagcttc tcaagatcgc ggcgaatggt ttcttcggtt acctgaaaaa
    11161 tcccactcaa atttgagact gtcacctttt tatcgttggc aaccatttgc ttaattgcct
    11221 gaatcctgtc ttttgccgcc acgattacac ccctgtatct ttttacatca cattagcgcg
    11281 attatcgcat aaccgatgtt tactttcaaa ataacctgtt tgaatcacag attttcatca
    11341 cagttttcac agaaacagag gtgaatcgtg ttgagtattt aacgctccag ggcctctgcc
    11401 atttccctca tcaatgttgc gtctgcatcc gataagttga tattcagtgc cgcgacattt
    11461 tcgcgtacct gttccggtgc agtagcccca ctaagaatgg agattaaatc actctgtttt
    11521 aatatccacg ccagtgccag agtgggaatt gtgcactgat aacgagcaca aagtggctgc
    11581 cactgttcaa gcatatcaat cactttcagc atgttttcac gctggaacca gactttattt
    11641 gcccgagcgc cgcccggaac gtaatcacga gtgatggtgc cggtcaacaa tccctgctct
    11701 agcggggaat aaacctgaac cacaatgcca ttatcacgac atagtggcag cagttcgttt
    11761 tccattgccc ggtcgaggat actgtatttc gcctgaataa tatccagttc accatattgc
    11821 agatactcgc ggatatggtc agcatcgacg ttagcagcgc ctatagcgcg aattttccct
    11881 tcagacttta actcattaag cactgcgaca gtttcagcga tcggcgtaaa aaatggcggc
    11941 accgactgcc agtgcgtcat gtagatatcg atgtaatcaa tacccagacg ttgcaagctc
    12001 gctgctacct cttcgcggat agattccggg gaaaggtttt tatacaactg ccgatcgcca
    12061 actttgttga ataaacttcc ttttcgttcc cagacaatgc cgcatttggt ttctactaca
    12121 acctgttcac ggggcagttt ttttaacgcc tgaccgacga taacttcact attgccaaag
    12181 ttatatcctg gcgcagtatc aatcagatta atgccacaac gatgggcttc aagaatagta
    12241 tcaatacata tttgccgatc gagatcgcca ttccatgcag gaccgccgcc aatggcccat
    12301 gtccccaacc ccattcgcga aagcgtaata tccgttgtgc ctaaaggtat ctttttcatt
    12361 tgccttcatt atccttcgta ttcttccagc aattgttcga ccagctttct gttttttacg
    12421 ccggtggtgg caccgacgct tagaaccgag atagccgccg ttgcattggc aaagcgtgcg
    12481 cattcacgca gatttttgcc ttctaacagt gccgcaataa aacctgaagc aaagttatcg
    12541 cccgcgccaa tggtgtcgat ggcggttatt cctgcgaccg ccggcacctt catcgtcatg
    12601 tcaccacgct tgataaagca gccgtcttta cccgttttaa tcaccaccgt ttttacgccg
    12661 cacgcaagaa agcagtcagc aatttcatcc agtgtctctt tcccggtgag taattttgcc
    12721 tcggcaaaat taggaaacag ataatcgaca taactcaatg cttcgcaaat atcatccagc
    12781 gtttcattca accgcggttt gatcatatcg gcacagataa tcatctgccg ggctttagct
    12841 tgcgtaaaaa tttctgttag cgctttacca tccaatagtg gactgttgaa aatactggcc
    12901 agtgataata atttcgcctg agaaaaccgc gcaaaatcaa catcgtcaat attcaatttc
    12961 cacagactgc cattacggtt tgtgacaaac gtccgctcgc catcctccgt caccagtcca
    13021 acgttaatag aggtatctat gctgacgtcc tgcttcaggc tttgaatatc aatattctct
    13081 ttgcggcaat ggtcgaaaat aaattgccct gcggcatctt taccaatacg actcattaat
    13141 gctgtgcgat ggcccagacg agaaataatt gttgcttcat ttattgcatc acccccggtg
    13201 gtcattgcga ttctttcaag aggataagaa tccacatcaa agatattttt actgaccggt
    13261 tgcaatggaa tatcaacaat agcggcacct atacaaataa cgtcgagatt atccatatca
    13321 ttattccgct ttgccatcag aaccgaacag tttaattttt tccaatgccc gttcttttac
    13381 agctttacgt acttcacgtt ctaaatgcag gaaaggctga tcctggtttt ctttcactgc
    13441 aaccattgcg gcctgacaga gctccgtatg aatgttgatt ttcgcgatac ccaacgaaat
    13501 tgcagtttta atgtcggcat cactgatccc cgatgcaccg tgcaaaacca gcggaacaga
    13561 aacggcatcg cgtacgcgtt tgacgacctc aaagttcaat tgtggctctg acgtataaac
    13621 cccatgctgg ttgccgatgg cgacagccaa tgaatcacag cccgtacgtt caacaaattc
    13681 agccgcctgg tcaggatcgg tatagtgata gcccgccagc gcttcctcat aaacggtttc
    13741 attaccgaca tgccctaatt ccgcctctac cggaataccc agcggatgga agaaatcgac
    13801 agcctctttg gttaaacgaa tattttcttc gaaatcaaac gcggaagcat cacgcattaa
    13861 tgaattcata ccatgagtcc aggcgttatg aataatctcc atactccgac catgatccca
    13921 atgagttatt accggcaccg ttgctttttg tgccattgat accatcatgt gagagaaatc
    13981 ttcaaatgag gtgttaccga caaaacctgt accaaaagaa ataataaccg gggatttcgc
    14041 ttcttcggct gcgtcgataa cgcccatcaa catttctgca ttccatacgt taaaatgggc
    14101 aattgcataa tgtttatttg tggcatcgtt ttcccaatat ctgatatctg cgagcatgtt
    14161 ctgttctccc gtattaatta atcgctaatt ttaataacgc ctttaataat gtcgcgtttg
    14221 ttgttaactg actcttcaaa tgcctgttgt acatcccgat aatcgtaaat atgcgtcacc
    14281 atcgatttca catcgaatcg ccctgaagaa atagcttcaa tcgtgaccgg ataacgattg
    14341 gcatagcgga ataccgtctg gatagtgact tcgcgattga ttttgaggaa attgattgcc
    14401 gaatcgccgg gtacagtacc aacaatcata attttaccgc cgcgcattac cagataaggt
    14461 gcctgtttaa cggtgaccgc agaacccgct gtttcgaaaa caatatctgc gcccatgtct
    14521 tcggtaaatt gctgacagcg tgcaatagtg tcttcttttg tgccgttaat aaccactgtc
    14581 gcaccaagtt gttccgccat tatcagacgt ttttccagca catcaacgac ggcaatttcc
    14641 gttgcgccca ggcatttgca cgcttgcaac gtcatcaaac caatacaacc tgctcccaga
    14701 ataattatct tcttacccgg tttaacatct gccagcatcg cggcatgcat cccgactgcg
    14761 gcaggctcca ccagcgcccc ttccatcgtg tccatattgt ctggcagttt gtaagtaaag
    14821 ctctccggat gacacagata gtgcgttaat gcgccgcggt agttgggttg tgtcgccata
    14881 aaatcaacgt ccgggcagat gttatatttg ccttccagac agtaacgaca gtgaccgcaa
    14941 ggaacgccag gttcgatatt tacccgatcc cccggtttaa atttgcgcac gcggcttccc
    15001 acagccacaa ccgtcccggc gcattcatga cccaggccaa tttcttgatt tgggtcttta
    15061 ggaggaataa acgggcctga ttcaaaacca tgtacatctg aaccacaaat accgacatat
    15121 tctactttaa tcaaaacttc atcttcttta ggcacaggta tttctgctga aataattttc
    15181 attgtgcccg gcacctgcaa tattgctttt gaatttttca ttttgaatcc accagtatta
    15241 tttattggct actgcatcaa tacttaaact ttcaacggag acacctttgg tttcaattcc
    15301 aatggtggcg atagcaattg cgacaataat tgaaacagcc cccagaagaa taaagactcc
    15361 cgttacgcca taactactga gcagcactgc aacggcataa ggtgcggcaa taccactgat
    15421 tcgccctacc gcattcgcca gaccggagcc acggagtttg gcctctgtcg gccagatttc
    15481 agggacatac actgccgagg catagcaaac gtacatatag acgaaagtaa tcaggaagaa
    15541 accaatcaac gttatgagca acatactggt ttgcagtgaa tagatatatc cgagcaccgc
    15601 aatcaggatt aatagcccca cacccattgt tttacgcgga attttatcca tcaccagcat
    15661 ggcaataaaa ataccaaatg gcgcgccaaa catactcatg gtatttaaaa caatcgagtc
    15721 ttttaaatta atcccctggg tcatgaatat tgttggcaac cagttaatta gtgtgtactg
    15781 cacaacgttc atggcaatca gcacacaaga gcctaatatc acgcgtttca gtaatactcc
    15841 tgtcagtaag gctgaatacg gcaccgcttg tggcgcttta ccgtcatcag caataaccac
    15901 aggcggcaaa ggtttacccg tctggcgtat gacgccttct tctatactcc gcatgacttt
    15961 ctcggcttcc tgataccgtc cgcgcgattc cagccagcgc ggggattcag gaaagtagcg
    16021 ccaggccagc gccgtagcga taagcgacaa tattgcaggg ataagcagtt gtacccgcca
    16081 gttccactct gcactaatca gcggcgtgag tcccatcgct atcaatgaac agagcggata
    16141 tgaccagttg ccaataaagg aaacccgact tgaccacgtt ccacgatttc taccgggcat
    16201 atattcggtg aaaccagcaa acagtgtaac cagtaaagct cccagcccaa cgcccatcac
    16261 aaaacggcag gcaataagaa aatccatatt cggtgagaaa gcaccgacaa ccatcgaggc
    16321 aatatgaatg gcctcataga ggatgaacgc atttctgcga ccggttttgt cgccaatgat
    16381 gccgcccacc agcgcaccga gaaacattcc ggcggtcgtg attgctgaga atgtggctgt
    16441 ggtggaatta tctgtccagc ccaacgcttt cagctgcgcg aggatcaagc caccaacggc
    16501 attactccag cagacaagca agccaaacgc gacgatggca aacattgacg aatgccagcg
    16561 gcaatccggt aagcgatcca gccgtgcacc acaatgcggt tttgttatct gttccatgcg
    16621 ataacgttcc ttcaggagag tggttattca tcaaagtcgt aagtcatgat cactttgatt
    16681 gcggttttat cgaccatcgc atcaaaccct tcgcgccatt gcgacaggcc gatacgatgc
    16741 gtgatcatcg gtttgacttt gatagcgccg ctggctaata gcctgatagc gttacgccat
    16801 gaggtggagt cataggccat atgcccaatg atgcttttgt tccaggcggt aatgtcatta
    16861 atcgagaaat caagaggttt gaagcccatt ccaacgcgta ccacttcccc gttcgggcgg
    16921 agcatttcga tggcttgttt cagtgcgata ttggcaccgg agcattcaat caccagtccc
    16981 agattatctt tgccacaaat ttgctggcag cgcgccacca catcttcggt agaaccattt
    17041 actactgccg tcgcacccag ttcttttgca accgggaagc ggaccgccac atcttcttgc
    17101 agaccaacga cgacgatatt taccgccccc ataattcgcg ccatttgtac ggagaacagc
    17161 ccgagtgggc cagtgccgat gacgaccaca tcctgaccag gaaggaattt cgattgctgc
    17221 gcgatggatt tgtaggcatt acagataggg tcaagtacgg ctgcgtcctc ataatcaaca
    17281 ccatcaggga tttcccacaa cgcatgacga tgaattttga gaatttcacc aggaaccaga
    17341 caatatttgg aaaaaccacc gccccaggta ttattatcca gaccaaggtt tactttttct
    17401 gtacaacaca gaaaatcacc ttgttcacag gccggacaaa cgccgcaaac gtgaccgctg
    17461 ttatccgaca caacgcgttg ccccactttc cagtctttga ctttttcacc aacctgcgca
    17521 atacaacctg cgaactcatg gccgcggata gagttaaact catcagaacc gctatcgaca
    17581 ttgtagtgct tcatgtctgc gccgcagatt gccgcggctt taatttcaat cactacgtct
    17641 tccgggccac aaatgggttg tgggacatca atcatcttgt agccgccaaa ggccttgcca
    17701 aaccgagcca gtgctttcat tgcgtcatcc tctgtgtttt gttttgctta gatggccatc
    17761 atacttaccc acatgatttt tgaggtcaac attaaatctg caaaacacag ataatgatct
    17821 gcgttttaca acttagatca caattacgca gaaaatggct gggtctgtta caggttggtg
    17881 gaaggcggaa cgcaaaaaga gcaaattcaa gagggattac cggccaaata ggtctgagac
    17941 ttaagtccag gagaagtcat tcaggcgtga acgcgacggc aagagatcgg actgggactt
    18001 agccaggtcg ggtcgagata attgagacga gtcgtggggt cattgttggc tatccccttc
    18061 atagccgcgg caaaaagggg atgaaccact tacttaaaca tggcaatagg ctttgctgag
    18121 ataccaaaat cttcttttaa ctgctgtttg ctcttcatga ccatctgacc atttgtgtca
    18181 attgtcatat gctgtgcttc ggtattatgg cgggcttgcc acagcatcac cagttgcagg
    18241 caattctctt tttgttcctc ggtcaacgca acgccatcag gccatttccc cagttcaacg
    18301 gcggtcgaca aacgctggta tacctcaggc gtcatgctgt tgataatgtc atcaagattc
    18361 ataatttttc cgctcctgtg gaataagttg ctgaatcgtt ttttcaaccg ttgatttctt
    18421 cgccgttttc gccatcggta aagcgtaaag aggcagagtt aacacaataa cgttcgcctg
    18481 ttggttgcgg cccgtcggga aagacatgcc ccagatgggc atcacagtta ccgcaacgaa
    18541 tttctatgcg ctgcattcca tgtgacaagt ctttgatata acgaatggat tcttcactca
    18601 ccggttcgta gaaactgggc cagccacagc cggaatcata cttggtttcg gaatgaaaca
    18661 gcggggcatc gcagatcaaa cagtgatata cgccgtcacg cttgttatgc agtaaacgac
    18721 ccgtaaatgg cggctctgtc ccatgattct gcgttacgta aaactgcatc tcggacaaat
    18781 tttttttcag ttcttctgcc gaaggtttat tagccatttg ctcacatctc actttaatcg
    18841 tgctcacatt acgtgactga ttctaacaaa acattaacac caactggcaa aattttgtcc
    18901 taaacttgat ctcgacgaaa tggctgcacc taaatcgtga tgaaaatcac atttttatcg
    18961 taattgccct ttaaaattcg gggcgccgac cccatgtggt ctcaagccca aaggaagagt
    19021 gaggcgagtc agtcgcgtaa tgcttaggca caggattgat ttgtcgcaat gattgacacg
    19081 attccgcttg acgctgcgta aggtttttgt aattttacag gcaacctttt attcactaac
    19141 aaatagctgg tggaatatat gactatcaaa gtaggtatca acggttttgg ccgtatcggt
    19201 cgcattgttt tccgtgctgc tcagaaacgt tctgacatcg agatcgttgc aatcaatgac
    19261 ctgttagacg ctgattacat ggcatacatg ctgaaatatg actccactca cggccgtttc
    19321 gacggtaccg ttgaagtgaa agacggtcat ctgatcgtta acggtaaaaa aatccgtgtt
    19381 accgctgaac gtgatccggc taacctgaaa tgggacgaag ttggtgttga cgttgtcgct
    19441 gaagcaactg gtctgttcct gactgacgaa actgctcgta aacacatcac cgctggtgcg
    19501 aagaaagtgg ttatgactgg tccgtctaaa gataacactc cgatgttcgt taaaggcgct
    19561 aacttcgaca aatatgctgg ccaggacatc gtttccaacg cttcctgcac caccaactgc
    19621 ctggctccgc tggctaaagt tatcaacgat aacttcggca tcatcgaagg tctgatgacc
    19681 accgttcacg ctactaccgc tactcagaaa accgttgatg gcccgtctca caaagactgg
    19741 cgcggcggcc gcggcgcttc ccagaacatc atcccgtcct ctaccggtgc tgctaaagct
    19801 gtaggtaaag tactgccaga actgaatggc aaactgactg gtatggcgtt ccgcgttccg
    19861 accccgaacg tatccgtagt tgacctgacc gttcgtctgg aaaaagctgc aacttacgag
    19921 cagatcaaag ctgccgttaa agctgctgct gaaggcgaaa tgaaaggcgt tctgggctac
    19981 accgaagatg acgtagtatc taccgatttc aacggcgaag tttgcacttc cgtgttcgat
    20041 gctaaagctg gtatcgctct gaacgacaac ttcgtgaaac tggtatcctg gtacgacaac
    20101 gaaaccggtt actccaacaa agttctggac ctgatcgctc acatctccaa ataagttgag
    20161 atgacactgt gatctaaaaa gagcgacttc ggtcgctctt ttttttacct gataaaatga
    20221 agttaaagga ctgcgtcatg attaagaaaa tttttgccct tccggtcatc gaacaaatct
    20281 cccctgtcct ctcccgtcgt aaactggatg aactggacct cattgtggtc gatcatcccc
    20341 aggtaaaagc ctcttttgca ttacagggcg cacaccttct ctcgtggaaa cctgcgggtg
    20401 aagaagaagt tctgtggttg agcaacaaca caccgttcaa aaatggcgtc gctattcgcg
    20461 gtggcgtacc ggtttgctgg ccgtggtttg gtccggcggc acaacaaggt ctgcctgcgc
    20521 acggttttgc ccgcaacctg ccgtggacgc tgaaatcaca tcgtgaagat gctgatggcg
    20581 tagcgctgac ttttgaattg acgcaaagcg aagagacgaa aaaattctgg ccgcacgact
    20641 ttacgctgtt agcgcatttc cgcgtgggta aaacctgtga aatcgatctt gaatcacatg
    20701 gcgaatttga aaccacctct gccctgcata cctactttaa cgtgggtgat atcgctaagg
    20761 taagcgtcag tgggctgggc gatcgcttca ttgataaagt aaatgacgcg aaagaagatg
    20821 tactgaccga cggtattcag accttccctg accgtaccga tcgcgtgtat ctgaatccgc
    20881 aagattgcag cgtgattaat gatgaagcgc tgaatcgtat tatcgccgta ggccaccagc
    20941 atcatctgaa cgttgtcggc tggaacccgg gaccggcgct ttcagttagc atgggcgaca
    21001 tgccggatga tggctacaaa acatttgttt gtgtagaaac ggcttacgct tcagaaacgc
    21061 aaaaagtgac caaagagaaa cctgcacatc tggcgcaatc cattcgcgtt gcgaaacgtt
    21121 aatttgcgtt aatgttgtgt gccgggtgcg atgcatccgg cacgtatcaa catcacacca
    21181 tatccagcgc agtttttcct tttggtgccg gatacgcttt atccagcatg gttaattccg
    21241 ctgacgaaag ttcgacctca agcacagccg cattctgttg gacatgggca atcgtggccg
    21301 cttttggaat cgccatcaca ccctgatggc tgattaccca cgccaacaat acttgtgccg
    21361 cgctgatatt gtgagcatgt gcaatttcgt tgactaccgc gttttttaac agtccattgc
    21421 gcaaccgccc ggcctgggct aatggactgt aagccatcac cggcatctgc tgttgctggc
    21481 accaggggag tagatcgtac tcaatccctc gtgaaccgag atggtaaagc acctgattag
    21541 tggcacactg atttcccccc ggcagctgcc agagttcctg catatcagca tgatcaaggt
    21601 tagaaacgcc ccagcggcgg atttttccct gggcgatcaa tttttccatc gctgccacag
    21661 tctcttcaaa agcgaaactg ccagaccagt gtaataagta gagatcgaga taatcagtat
    21721 tgagacggcg taaactggct tcgcatgcat ttatcgcttt ttgcccgcca gcattccacg
    21781 gatagacttt agagacgaga aagacgttat cgcgcagacc ggttaatgct tccccaacca
    21841 ccttttcggc accgccatcg gcatacattt cggcggtatc aatgagggtt aaaccgagct
    21901 caatgcccgc gcgtagtgca gcaacttctg ttttgcgctg actggcatct tcgcccatat
    21961 accatgttcc ctgccctatg gctggcagtg agacatcgcc actaaattga atcatttttt
    22021 gttgcattgt ttcctcccag gtattgcacc accgtaatgc aaaacagggc gtgacgccct
    22081 gtttttatgc acaaaatgcc ctggaaagat gcattatcag aatttgtagg tgatcccggt
    22141 agaaatcagg ccagtccagg atttatccac catcgggctg tcagtaactt catcagacag
    22201 acgggtatag cgcgcggtac cgtaaacact ccagtcgccg aggaagttgt agctggcgct
    22261 cagctccagg taagggctcc agctgtcgtt cgggttatag ccacgcagac cgctgcgagc
    22321 ggactctttg cgcgatacgc cataatagta ttcgttctgg ttttcgctgt tccactgcac
    22381 accaataccc ggagtcacgg tcaggccacc gttggtgtaa cgatacaacc aggccatatc
    22441 ccagacaatg ccgttgctgt tatccagggt atcgccagcc agggtggtac gcaggtaacc
    22501 gtactgggta aagtgagcat aagacagacc agccatcatg gtgctcttac ggtcatccag
    22561 gtgacgcatt tggtgatcgc cactgtcttt cgctttgaag taaagcggcg accagtaagc
    22621 ggtaattgaa agtttatccg ttgcgtcatt ccacaggtag taaccaccac ctaagccacg
    22681 gaaccagaag ttatcgcctt catagttgat taccggtact gggtaaacat cggtatcgta
    22741 atctttatat gggtgctcaa cgacacctac gcctgcgccc agggaaaatt taccttcagc
    22801 gtgcgctacg cctgcagacg ttgcgataag cactccaagt gccagaagtt tgagtttggt
    22861 cacaattaat cattccttaa acaaatgttt agcgggcgac aaagtttacc cgtcaataca
    22921 tcgaaaccca acctttttac gttttcattt tttaaagtaa ctgtttaatt ttcctgacgc
    22981 ggatgacacc gcgcttacag ccaaatgaat ttagcgttac tggctaccct ggtctttaca
    23041 ttaattatgc aaaatttatg gatgagttgt tgatatgcca ttgaaattaa gaaagccgtg
    23101 caggcaagtt ttccatttgc catctacgct taattttgaa ggtgtatcac cgggcacgtt
    23161 gttctcatcg tcgataaaat ggcatgagag ttgctgtgtt ttagcaagag acgtcgttca
    23221 gtttacctct tccgggagcc tctactattc atatgaacgg ctcttaacct gtgctaaaaa
    23281 acgaaaggac ggcataccat gaatatattc gatcactatc gccagcgata tgaagctgcc
    23341 aaggacgaag agttcacact gcaggagttt cttaccactt gtcggcaaga tcgcagtgct
    23401 tatgccaacg ctgctgagcg gctattgatg gctatcggtg agcctgtcat ggtcgataca
    23461 gcccaggaac ccagactttc tcgactcttt tctaaccggg tcattgcacg ttatccggca
    23521 tttgaagagt tttacggcat ggaagacgcg attgaacaga ttgtctctta tctgaaacac
    23581 gcggctcagg ggctggaaga gaagaaacaa atcctgtatc tgctggggcc tgtgggtggg
    23641 ggtaaatcat cgcttgctga gcgactgaaa tcattaatgc agctcgtacc gatttatgta
    23701 ttgagcgcca acggtgagcg tagcccggtc aacgatcatc cgttctgtct tttcaatccg
    23761 caggaagatg ctcagattct ggaaaaagag tatggcattc ctcgccgtta tctcggcacc
    23821 atcatgtcgc catgggcggc aaaacgcctg catgaatttg gtggcgatat cactaagttc
    23881 cgggtagtga aggtctggcc gtcaattctg caacaaattg ctatcgccaa aacggaaccc
    23941 ggtgatgaga acaaccagga catctccgcg ctggttggga aagtcgatat tcgtaaactc
    24001 gaacactacg cgcagaatga cccggacgcc tacggctatt ccggtgcgct gtgccgcgcc
    24061 aaccaaggga tcatggaatt tgtcgagatg tttaaagcac cgataaaagt gctgcatccg
    24121 ctgttaaccg ccactcagga aggtaactac aacgggacgg aaggtatctc cgccctgccg
    24181 ttcaacggga ttattctcgc ccactcgaac gaatccgaat gggtcacttt ccgtaataac
    24241 aaaaacaacg aagccttcct cgaccgtgtt tacatcgtga aggtgccgta ttgcttacgc
    24301 atttccgaag agatcaaaat ctacgagaaa ttgcttaatc acagtgaatt gactcacgcc
    24361 ccctgcgccc ctggcacgct ggaaacgctg tcacgttttt ccattctttc gcgccttaaa
    24421 gagccagaaa actccagcat ttattcaaag atgcgggttt atgatggcga aagtctgaaa
    24481 gacaccgatc ccaaagccaa gtcgtatcag gaatatcgtg actacgccgg tgtcgatgaa
    24541 gggatgaacg gtctgtcgac gcgttttgcg tttaagatcc tctcccgcgt gttcaacttc
    24601 gatcatgtag aagtggcggc aaacccggtc catctgttct acgtactgga acagcagatc
    24661 gagcgcgagc agttcccaca agagcaggca gaacgctatc tggagttcct gaaaggttat
    24721 ctgatcccga aatatgccga gtttatcggc aaagagatcc agacggccta ccttgaatcc
    24781 tattccgaat atgggcaaaa cattttcgac cgttatgtta cctacgcgga tttctggatt
    24841 caggatcagg agtatcgcga tccggatacc gggcagctgt ttgaccgcga gtctcttaac
    24901 gccgagctgg agaaaatcga gaaaccggcg gggatcagta atccaaaaga tttccgcaac
    24961 gagattgtta acttcgtact gcgcgccaga gcgaataaca gcggacgcaa tccgaactgg
    25021 accagctatg aaaaactgcg cacggttatc gagaagaaaa tgttctccaa taccgaggag
    25081 ctgttgccgg ttatctcgtt taacgccaaa acgtcaaccg acgagcagaa gaaacacgac
    25141 gactttgtcg accgtatgat ggaaaaaggc tacacccgta aacaggtgcg tttactgtgc
    25201 gaatggtatt tgcgcgtacg taaatcgtct taacaaccct ggcccggtac acatgttacc
    25261 gggcctacaa cgacagcgaa ccgtgggcct gagaagcggc aacacaggcg tagcatacag
    25321 ttggcaaatg tagtacgggg ggcatatgac ctggtttatt gaccggcgtc tgaacggcaa
    25381 aaacaaaagc atggtgaatc gccagcgttt tttacgccgt tataaagcgc aaattaaaca
    25441 gtcgatctcc gaggccatta ataagcgttc ggtgactgac gtcgacagcg gcgaatccgt
    25501 atccattccc acggaagata ttagcgaacc gatgtttcat caggggcgtg gcggtctgcg
    25561 ccaccgcgtg catccgggca atgaccattt cgtccagaac gaccgaattg aacgtcctca
    25621 gggtggcggc ggaggttccg gcagtggtca gggccaggcc agccaggatg gtgaaggtca
    25681 ggatgaattt gtctttcaga tttcgaaaga tgagtatctt gatttgctct ttgaagattt
    25741 ggccttaccg aatctgaaac aaaaccaaca acgccagctg accgaatata aaacgcatcg
    25801 ggcgggttat accgctaacg gcgttccggc aaatatcagc gttgtgcgct ccttgcagaa
    25861 ctcactggcg cgacgcacag ccatgacggc aggcaagcgg cgggaacttc atgcactgga
    25921 agagaatttg gccatcatca gcaacagtga acctgcgcag ctgctggaag aggaacgtct
    25981 gcgcaaagaa attgcagaat tacgtgccaa aattgaacgc gtccctttta ttgacacctt
    26041 cgatttacgt tacaagaact acgagaagcg gcccgatccc tccagccagg cagtgatgtt
    26101 ttgcctgatg gacgtttccg gttcaatgga tcaatccact aaagatatgg ctaagcgttt
    26161 ttatattctg ctgtatctgt tcctcagcag aacgtataag aacgtggaag tcgtatacat
    26221 ccgccatcat acccaggcga aagaagtcga tgaacatgag tttttctact cgcaggaaac
    26281 aggcggcacc atcgtttcca gcgccctgaa actgatggat gaggtagtga aagagcgtta
    26341 taacccggca cagtggaata tttacgctgc acaagcatcg gacggcgata actgggccga
    26401 tgactctccg ctttgccatg aaatcctggc gaaaaaatta ttacctgttg ttcgttatta
    26461 cagctatatc gaaattaccc gtcgtgcaca tcagacattg tggcgagaat atgagcatct
    26521 gcaatctact ttcgacaact ttgcgatgca gcacatccgc gaccaggatg atatttatcc
    26581 ggtgttccgt gaactgtttc ataaacaaaa tgcaacagct aaagactaaa actatcagcc
    26641 aggtcattat cgcctggctg atttttagct tactgtaaat tatctcattt attacataca
    26701 ttagcttaca at
//
LOCUS       DAZFRN010000059        26309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFRN010000059 DAZFRN010000000
VERSION     DAZFRN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.059"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1149
                     /gene="yjgN"
                     /locus_tag="ACHKUB_002378"
     CDS             <1..1149
                     /gene="yjgN"
                     /locus_tag="ACHKUB_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO6655390.1"
                     /translation="FHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLYANMEV
                     NGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCVLTLLLLVLLIFMAAKG
                     LRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSV
                     IISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILAF
                     LALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAV
                     STSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLK
                     IWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIMPSLPFL"
     gene            complement(1205..4060)
                     /gene="valS"
                     /locus_tag="ACHKUB_002379"
     CDS             complement(1205..4060)
                     /gene="valS"
                     /locus_tag="ACHKUB_002379"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO6655391.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(4060..4503)
                     /gene="holC"
                     /locus_tag="ACHKUB_002380"
     CDS             complement(4060..4503)
                     /gene="holC"
                     /locus_tag="ACHKUB_002380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO6655392.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(4637..6148)
                     /gene="pepA"
                     /locus_tag="ACHKUB_002381"
     CDS             complement(4637..6148)
                     /gene="pepA"
                     /locus_tag="ACHKUB_002381"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO6655393.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            6302..6352
                     /gene="ytgA"
                     /locus_tag="ACHKUB_002382"
     CDS             6302..6352
                     /gene="ytgA"
                     /locus_tag="ACHKUB_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO6655394.1"
                     /translation="MSIIILATISTTQENK"
     gene            6415..7515
                     /gene="lptF"
                     /locus_tag="ACHKUB_002383"
     CDS             6415..7515
                     /gene="lptF"
                     /locus_tag="ACHKUB_002383"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6655395.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7515..8597
                     /gene="lptG"
                     /locus_tag="ACHKUB_002384"
     CDS             7515..8597
                     /gene="lptG"
                     /locus_tag="ACHKUB_002384"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6655396.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8758..10260)
                     /gene="yjgR"
                     /locus_tag="ACHKUB_002385"
     CDS             complement(8758..10260)
                     /gene="yjgR"
                     /locus_tag="ACHKUB_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO6655397.1"
                     /translation="MSEPLLIARTPDTELFLLPAMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRPNPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGVLGSLLGGRRR"
     gene            complement(10338..11336)
                     /gene="idnR"
                     /locus_tag="ACHKUB_002386"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(10338..11336)
                     /gene="idnR"
                     /locus_tag="ACHKUB_002386"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO6655398.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRTAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(11403..12722)
                     /gene="idnT"
                     /locus_tag="ACHKUB_002387"
     CDS             complement(11403..12722)
                     /gene="idnT"
                     /locus_tag="ACHKUB_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO6655399.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12785..13549)
                     /gene="idnO"
                     /locus_tag="ACHKUB_002388"
     CDS             complement(12785..13549)
                     /gene="idnO"
                     /locus_tag="ACHKUB_002388"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO6655400.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(13573..14604)
                     /gene="idnD"
                     /locus_tag="ACHKUB_002389"
     CDS             complement(13573..14604)
                     /gene="idnD"
                     /locus_tag="ACHKUB_002389"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO6655401.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14821..15384
                     /gene="idnK"
                     /locus_tag="ACHKUB_002390"
     CDS             14821..15384
                     /gene="idnK"
                     /locus_tag="ACHKUB_002390"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO6655402.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQEDEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(15388..16407)
                     /gene="ahr"
                     /locus_tag="ACHKUB_002391"
     CDS             complement(15388..16407)
                     /gene="ahr"
                     /locus_tag="ACHKUB_002391"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO6655403.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDIEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            16603..16687
                     /locus_tag="ACHKUB_002392"
     tRNA            16603..16687
                     /locus_tag="ACHKUB_002392"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16637..16639,aa:Leu,seq:caa)
     gene            16887..18155
                     /locus_tag="ACHKUB_002393"
     CDS             16887..18155
                     /locus_tag="ACHKUB_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6655404.1"
                     /translation="MALTDTKVRSAKPQEKEYTLVDGDGMFLLIHPNGSKYWRFRFRF
                     GGKQHLMAFGVYPETSLADARQKREEARRLVAAGIDPREHKRAVKEEQAKEAITFESV
                     AREWHATNKKWSEEHSRRVLKSLEDNLFSAIGRRSIEELKTRDLLAPIKIVEATGRFE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRVHRPALELKRLPELLHRID
                     GYTGRPLTRLAVELTLLIFIRSSELRFARWSEIDFDTAMWTIPAEREAIEGVKHSQRG
                     SKMRTPHLVPLSHQALAILKQVHKLSGERDFVFIGDHDPRKPMSENTVNKALRVMGYD
                     TRVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLDERRLM
                     LQWWADFLDVNREKVISPFDFAKIFASLKR"
     gene            18365..20014
                     /gene="dptF"
                     /locus_tag="ACHKUB_002394"
     CDS             18365..20014
                     /gene="dptF"
                     /locus_tag="ACHKUB_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptF"
                     /protein_id="HFO6655405.1"
                     /translation="MSAITLRKALGVLAKSSSFSVTTETHRQKDVFDELKEQLFVKQE
                     IEEELQRYLDTATPGEIIFLCGSSGDGKSEILTRCKSDPQYQQKFSFHLDATHSFAPQ
                     QSAIDALNDLFENHHSQSYPLLIGINTGMLANFAREGSESHMAIRAAIDSFLSAQQEG
                     QRPYRNENCIFFDFEHYPKFEFDEEREYSPFIKKLMNQLTGNDDKNLFYTIYQRDESI
                     GHDLKTVANFKLLCMPGVQNVLITQLFKARLIKDQFVTTRTLLDFLHHLLTGPGYLFD
                     NLFNGAENDLIKKVSDFDPARLHTYEIDQFILRYELGLVDPELDDFLSTLAKLHLTFD
                     RQCVKPGDAASLIRLFWLLQHESLGNNYHKRFSVFFKEALFERYSEIWHLHKNYTADP
                     EQKRTLNRFYSFELIAGIQRYANRKAPELSTQKEEFFLGEFGGIKITAPVALKPDWDS
                     ILKKNTAHTTCFDIYLKVGQDPLDPIRIGLNLFELLSKLNNGYRPNKYDKSAIVLLDE
                     IVELIAEKAKSSSEIKFYDGLKRVYSARADDDMITISGMEG"
     gene            20019..21344
                     /gene="dptG"
                     /locus_tag="ACHKUB_002395"
     CDS             20019..21344
                     /gene="dptG"
                     /locus_tag="ACHKUB_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HFO6655406.1"
                     /translation="MYPIATNLKVSNNQLDSYLPIRNKNNNIDWQIVTGLVLSYAVKY
                     KIDTYSLEQFREDCKTHLQILIDEPAFLSVLERMYFSSQDIFRVSPLFLLFHAQFDGE
                     KISAGSTADKRLGTLFANLMRDFSLNNPIQDKLNFIEKEMLNKLNKKLIRLGEGPFAK
                     EQPYLPYLVTCFQSDLAFLAEHPQYLLQELTNTLRLYAFSWCAQLALNLDNWQDGEPQ
                     SKSLFFILDTEKASSERDKIKLFGYKWFARQSEKLFPVLSALEVLQVKGEEKRPLWQV
                     YQDCLGYSDTSNRVLNELNNYIQKFISKEERDLPERDRATNLEDAFKQLLSVAVEQFQ
                     GKKTERAAVNRKYINELESQICTDFIQVRGRAGKVLVLNQDRLLLLTNLTVGKNKKLR
                     LHELLRGFEQRGFYLDNQSTQMLVAFYERMGNVERMSDSGDAVYVRKTV"
     gene            21325..>26309
                     /gene="dptH"
                     /locus_tag="ACHKUB_002396"
     CDS             21325..>26309
                     /gene="dptH"
                     /locus_tag="ACHKUB_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptH"
                     /protein_id="HFO6655407.1"
                     /translation="MSVKQYETYLAETFIEWVSSIIQPGERYQFKSPDPDNALKLWKA
                     FDSLANGNKLEIAPEQFLTYVQCNNIQLIPVLHGTTTPAFTENYISHLRDQVSGRNGI
                     FAQTALLIIHNSMLDTLLNSTKDVAAPDAIWHPETFRHQLEKLITTDSARSQVSRCLL
                     GDQLTTILDEGATVFGFSSLYRLLDDGNLDFSELKLFNDNNLLNFSDKQLRARLNENQ
                     KLYRQIEDSVERYSGQLENVLTEFSAKFIQEHFVDNDDWRELDFSVYLNEKAQNSEQK
                     LVLENITAENGEVWQRAKSTSKAGKRDISVLVQVLPGQSQVELEFIFQGNDLQDNQVK
                     IAHHRPLQKERFWRTSRLGGRNSKILASVPFDGRPCFFSLEIINRNNSAEEYKFRLLL
                     VEQGQFWLNEIQHCYRIEPGKGQLTLQLEDNELRIAEFGDKTCLLDEESDDVDCRHYA
                     LVNFETLANQSELIKFTLISGDSRLLFNIEGPGAEEGLTLPLLFDQGRFNKLFKEEGN
                     ATWNKLKGRVILDNIEHNVVGVRQQLLMQEYALIERNLLSTGSNGSEFALDELLASYP
                     DLHNAYHQLFVYYQRRNTLPSLVSWSAEYRVLVSHMIATFEQALQQISLSRALTIQEK
                     RLLHLGICRGDDYERLSPLHPLVMAYHLQLAETIIAEPGYPTSASFASLPEITLDRLV
                     VSGLMPFVYHSEHEYAQLQPMVENRFWIDIVPQRQMSHEYVKRLVKDKLNEFTDAYSR
                     LFQSAGNNALVINAINMGEARELFLGLVDYFKQEKDNAISIHVNCYDERLLPNAFDRF
                     AESGSYEQLKNDLGLNSGAWRSEADMLIDLLRSRLTFSKFILPPESDQLAYAHLAFFT
                     NTAPVDCRQIRIEDAASGVLCHGLIAGEGAETQGDAYFTAFGLRNVDTESSCALRLAR
                     LLGCLWQPARQSNSQYHGQGISLAVSGNFKQLLNRSYDSSLWTTIIDPKVTLDFFTSQ
                     KDVVLIHYSDQYTSCAGYDAVTVTKQVELFLRLLQTGQSTVDSQQLLAEFNAFNGEWL
                     LKMLRSNEKERKEKHGIIGAYKFVQSMLLQSDICWIPLSVAEMIRVSGNVGLRMKESD
                     LSRHLQGYQKGAISDDVLFVGFKDNNLYLLPLEVKTGARPDYNHAGQQARELKRYLQQ
                     DILGPKTLASQLYRALFIRQVLMQVEKLRLYGVLDNDKLSPLLERREWWLTGDYQLGE
                     LADYAEGFVVAHVDSGTCFDLSYKETAENILQIEIPYSLLPSLIAAQGDQPPLAERYR
                     VPEKYLLKPECDVNSSHPVQVATTTEPPPISALELPQPEASALTPSNVRNTPLQILFG
                     HDAVRQNPLYWEPTNTAKFMNTNTGIIGTMGTGKTQFTKSLVTQLMRNQSYNVDGKPI
                     GLLIFDYKSDYVDDAFLEATGAKRYQLSLLPYNPLSLFGDMPMLPRHTAMAFAETMGK
                     AYNLGVKQRMKLVTLIMECYDLAGIVPHDRSTWNRVAPTIEDVWQQYLAQEKVDEDSL
                     YAALYNLAGFQIFETDPEKMTSLYDLVDGVTVIELAGYPSEIQNLVVALTLDLFYAQM
                     QKRGKPTVRGDYRQLTKMILVDEADNFMRQDFASLRKILKEGREYGVGAILSTQEITH
                     FKTGENNYASYILTWVIHRVSEIRNSDIKAVFNIDDKSEQESLMGQIRQLE"
BASE COUNT         6739 a         6379 c         6358 g         6833 t
ORIGIN      
        1 tttcatggta caggtgagag atattttctt atttgtgtgg tgaatgtgtt gttaacgatt
       61 ataacgctag gtatctattt gccatgggca ttaatgaaat gtaagcgtta tctctatgct
      121 aatatggaag ttaacggaca acgattttct tatggaatta ctggtgggaa tgtttttgtt
      181 agttgtcttg tttttgtttt ttgctatttc gcaatcttaa tgacagtgtc agcagatatg
      241 ccacttgttg gctgtgtttt gactttgtta ctgttggttt tgcttatatt tatggcagca
      301 aaaggactgc gttatcaggc cttgatgacc agtctcaacg gcgtaagatt tagttttaat
      361 tgctctatga aagggttctg gtgggtaacc tttttcttgc cgattttaat ggccattggg
      421 atggggactg ttttctttat ctcgacaaag atgctacatg ccaatagttc aagtagtgtt
      481 attatatctg tggttctgat ggcaatagtt ggtattgttt ccattggtat ttttaatggt
      541 actttatata gcctggtaat gagttttctc tggagcaata ccagtttcgg tatacatcgt
      601 ttcaaggtga aattagatac tacgtattgt ataaaatatg ccattctcgc atttttagct
      661 ttattacctt ttctcgctgt tgctggttat attatcttcg atcaaatatt aaatgcgtat
      721 gatagttctg tgtatgcaaa tgatgatatt gagaatttac agcaatttat ggaaatgcaa
      781 cgtaaaatga taatcgcgca gttaatctat tattttggga ttgctgttag caccagttat
      841 ttaacggtgt cgttgcgaaa tcattttatg agcaacctgt cactgaatga tgggcgtatt
      901 cgttttcgct caactttaac gtaccacggt atgctttatc gcatgtgtgc gttggtggtg
      961 atatccggga ttacgggcgg tctggcttat ccactgctga aaatatggat gattgactgg
     1021 caggcaaaaa atacgtattt gctgggcgat ttggatgacc ttcctttaat caataaagaa
     1081 gaacaaccag ataaaggctt cttagccagg atttcacggg gaattatgcc ttctttacca
     1141 tttctgtaag agataaaaaa ggccggagca tgctccggcc ttcgttttca ttactgtgtt
     1201 ttgattacag cgcggcgata acagcctgct gttcaatcag cttcgctttc gcttctgcat
     1261 agccttccag cttttcacgc tctttcgcga tgaccgcttc cggtgcgcgg gcgacaaagc
     1321 cttcattcgc cagcttgttc tcgatacggc tgatttcgcc ttcgatcttc gccacttctt
     1381 tcgccagacg cgccagctca tcttctttgt tgatgaggcc agccatcggg atcagcagct
     1441 ctgcgccgtc gatgatctta gtaacggaaa ccggaccttt gtcatcggca ggcagcacgg
     1501 tgatgctttc cagacgcgcc agcgtttgca ggaagccacg gttttcattt acgcgacgtt
     1561 ctgcatccgc gctgcaacca cgcagtagca gctccagcgg tttgcccggc gcgatgttca
     1621 tttctgcacg gatgttacgt accgcaacga tcgcctgctt cagccactct gtgtcggcca
     1681 gtgcggcttc atcaacctga gatgcatcgt actgcgggaa cggctgcagc atgatggtgt
     1741 cggcagtgat accgcaaagt actttcacac gctgccagat ggtttcggtg atgaacggaa
     1801 tgatcggatg cgcgaggcgc agcagacctt ccagcacggt caccagcgta tggcgagtac
     1861 cgcgcagttc ggcttcggtg ccaccgttca ttaccggctt agtcagttcc agataccagt
     1921 cacagaactg gttccaggtg aactcataca gaatgcctgc ggcgatatcg aagcggaagc
     1981 tgtccagcgc ttcgcggtac gctttgatgg tctggttgaa ctccgccaga atccagcgat
     2041 ccgccagcga cagcgtcatt tcgccgccgt tgaagccgca atcctgacct tctgtgttca
     2101 tcagcacaaa gcggctggcg ttccacagct tgttacagaa gttacggtaa ccttccaggc
     2161 gtttcatatc ccagttgata tcgcgaccgg tagaagccag cgccgccagg gtgaagcgca
     2221 gcgcgtcagt accgtgcggt tcaataccgt tcgggaactg cttctcggtg cgcttacgga
     2281 ttttgtccgc cagctgtggc tgcatcatat tgccggtgcg tttttccagc agttctggca
     2341 gcgaaatacc gtcaaccata tccagtgggt caataacgtt acccttggat ttggacatct
     2401 tctggccttc gtcgtcacga atcaggccgg tcatgtaaac agtgtggaac ggcacctgcg
     2461 gtttgccatt ttcatctttg atgaagtgca tggtcatcat gatcatgcgg gcaatccaga
     2521 agaagatgat gtcgaaacca gataccatca cgctggttgg gtggaactga cgcagggcgt
     2581 cggtattttc cggccagcca agggtagaga aggtccacag cgcagaagag aaccaggtgt
     2641 cgagaacgtc ttcgtcctga cgcaggacaa catcagcacc gaggttattt tctttacgca
     2701 cttcgtcttc gttgcggcca acataaacgt tacccgcttc gtcataccat gccgggatac
     2761 ggtgacccca ccacaactga cgagagatac accagtcctg aatatcgcgc atccaggaga
     2821 agtacatgtt ttcgtactgc ttcggtacga actgaatgtc gccgttctca accgcttcaa
     2881 ctgccggttt cgccaggaca tcggcacgca cgtaccactg gtcggtcagc atcggttcga
     2941 tgaccacgcc accacggtcg ccataaggaa cggtcaggtc atgtggttta atttcttcca
     3001 gcaggccgag tgcgtcaacc gcagcaacga ccgctttacg tgcagcaaaa cgctccagtt
     3061 tctggaattc tgcggggatc tcgctggaat aaacgtcaga ttcgttacct ttggtatcga
     3121 acacctgggc actttcacgg atatcgccgt caaaggtcag gatgttgatc atcggcaggg
     3181 cgtgacgttt accgacttca tagtcgttaa agtcgtgcgc cggggtgatc ttcacgcagc
     3241 cggtgccttt ttccatgtcg gcgtgttcgt cgccaacgat cggaatacga cggttaacca
     3301 gcggcagaat gacatatttg ccaatcagat ctttgtaacg cggatcttcc gggttaacgg
     3361 ccacgccggt atcgcccagc agggtttccg gacgagtagt cgcaaccacc agataatctt
     3421 taccatctgc ggttttcgcg ccgtcagcca gcggatagcg gatgtgccac atcgaacctt
     3481 tcgattcgcg gttttccact tccaggtcag agatagcggt gcgcagtttc ggatcccagt
     3541 ttaccaggcg tttgccacgg taaatcaggt cttctttata cagacgaacg aaaacttctt
     3601 tcaccgcatt ggacaggcct tcgtccatgg tgaagcgttc acgctcccag tccacggagt
     3661 tgccgagacg gcgcatctga cgggtaatgg tgccgccgga ttccgctttc cattcccaga
     3721 ttttgtcgat gaaagcttcg cggccgtagt cgtgacgggt tttaccttct tctgcggcaa
     3781 tcttgcgctc aacgaccatc tgggtagcga tccctgcgtg gtcagtaccg acctgccaca
     3841 gggtgttttt gccctgcatg cgctgatagc ggatcatggt atccatgatg gtttgctgga
     3901 aggcgtgacc catatgcaaa ctgccggtga cgttcggcgg cgggatcatg atgcagaaac
     3961 tttcctggct ttcatcgcca ttaggcttaa agtagccctg cttttcccag tgctcgtaaa
     4021 gcggctgttc gatatcttgt gggttatatg tcttttccat tatttccagg ttgccgtatt
     4081 caggttgaaa ccagccacgc ggtaggcttt atagcgttcg cgcgccagtt gtttcagaga
     4141 atcttcataa ggaacgaagt ctaccacttc tgtgaaagcg gtggcaaaat ctgcaaagct
     4201 tgttcgcaga ctaatcaata tatcgcgcgg gctgctgctg cgtttttgcg gccaggcgat
     4261 ctccaccggc gcaccgccgc gcggtccttc tcccgctaaa ttatgcggaa caaagctttc
     4321 tgccggacgc gcccacaggg cttcatccag ccggtaagcc tgcttttcat cttcacaggc
     4381 gatgagcacg cgcttaccac tgcgccaacg ttctgcggca atttcacaca ccagctgctc
     4441 aacggcgctt aagccatcga cggtggtgtc attgtccaga aggtagaacg tcgcgttttt
     4501 cataaatggg gcttcttgtg gtggatttaa atattgctgc attcctcacc ctaaaagatg
     4561 agcaatgaat ttctattgcg ctcgatcggt tccctctccc ttagggagag ggttagggtg
     4621 agggtaaaac gcaaacttac tcttcgccgt taaacccggc gcggtttaac aggaactgtg
     4681 ccagcaacgc taccggacga ccggttgcgc ctttcgcttt accagaacgc caggcggtac
     4741 cggcgatatc caggtgcgcc cagttgtact tacgggtaaa gcgtgacagg aagcaacctg
     4801 cggtaatcgc cccaccagga cgaccgccaa tgttcgccat atcggcaaaa ttggactcca
     4861 gttgttcctg atactcgtca cccagcggta agcgccatgc gcggtcaccg gattgttcag
     4921 acgcggcaat cagttcatgg gccagcggat tgtggttcgc catcagacca gtaatgtgat
     4981 gacccagcgc gatcacgcag gcaccggtca gcgtcgccac gtcaatcacc gcttccggct
     5041 caaaacgctc aacgtaagtt aacacgtcgc acagtaccag gcggccttca gcgtcggtgt
     5101 tcagcacttc aacggtttga ccggacatgg tggttaacac atcgcccgga cgataggcac
     5161 gcccgccagg catgttttcg cagccagcca gcacgccgat aacgttaatc ggcagttgta
     5221 gctccgcgac catccgcatc acgccgtaaa ccgctgccgc accgcacata tcgtacttca
     5281 tctcatccat gccttctgaa ggcttgatcg agataccgcc ggagtcgaag gttaaacctt
     5341 tacccaccag cacgattggg cgtgcatctt ccgacgcgtt gcctttgtac tcaatcaccg
     5401 acatcagcga ttcgttctgc gaaccctgac cgaccgccag ataggaatgc atccccagct
     5461 ctttcatctg ttgttcgccg ataacgcggg taatgacatt cttgctgtag ctgtcagcca
     5521 gttggcgcgc ctgagaggcg aggtaagcgg cgttacagat attcggcggc atattgccga
     5581 gatcttttgc tgctttaatc ccggcggcaa tcgccagacc gtgctggatc gcgcgctcac
     5641 cgctggtcag ttcacggcgg gtcggcacgt tgaacaccat tttacgcagc ggacgacgcg
     5701 gttcgctctt gttcgttttc agctgatcga aactgtagag cgtctctttt gccgtctcga
     5761 cagcctgacg cactttccag tagttgttac ggcctttaac gtgcagttca gtcagaaagc
     5821 agaccgcttc cattgagcca gtatcattca gcgtattaat ggttttctga ataacctgct
     5881 tgtactgacg ctcatccagc tcacgttctt tgccgcaacc aataaggaga attcgctcgg
     5941 aaagtacatt cggaacatgg tgcagcaaca atgtctgccc cggttttcct tccagttcgc
     6001 cccgacgtag cagggcgctg atgtacccat cgctgatttt atcgagctgt tctgcaatcg
     6061 gagaaaggcg acgtggttcg aagacgccca cgacgatgca ggcactccgc tgtttctccg
     6121 ggctaccgct ttttacacta aactccatgc actacgctcc tgaatcttaa agacaacggc
     6181 ggtggctaca gatagaattg caagctttcg taactcatgt ccgctgttgc gatgacttcg
     6241 tgttaatctt aacgttatta cggcattggc acgtcagaac aaaatctgag aggtgaatcc
     6301 gttgagtata attatcttag cgacgatttc gacgactcaa gagaataaat gacgtttaag
     6361 ccatgaaaca agctaaaatc ctgcaaaaga cgagttttta cgggcgtatt taaagtgata
     6421 atcataagat atctggtgcg ggagacgctc aaaagccagc tggcgatact cttcatcttg
     6481 cttttgatct tcttctgtca aaagttagtg aggatcctcg gcgcagcggt tgacggcgat
     6541 attccggcga atctggtgct ctcccttctc gggttgggcg tgccggaaat ggcgcagctt
     6601 atcctgccat taagcctgtt cctcgggctg ctgatgacgc tgggcaaact gtataccgaa
     6661 agtgaaatta cggtaatgca tgcctgcggc ctgagcaaag cggttctggt gaaagcggca
     6721 atgatccttg cggtattcac ggcaatcgtc gcggcggtta acgtgatgtg ggcgggaccg
     6781 tggtcatcgc gtcatcagga tgaagtgtta gcagaagcga aagcgaaccc tggcatggcg
     6841 gcgctggcgc aagggcaatt ccagcaagcg actaatggca gttcggtgct gttcatcgaa
     6901 agcgttgacg gcagcgattt caaagatgtg ttcctcgcgc aaattcgacc aaaaggtaat
     6961 gcacgtcctt ctgtggtggt ggccgattcc ggacatttaa cccagctgcg cgacggctcc
     7021 caggtcgtca ctctcaacca gggaacgcgc ttcgaaggca ctgcactgtt acgtgatttc
     7081 cgcattacgg atttccagga ttatcaggcg atcattggtc accaggcggt ggcgctcgac
     7141 ccgaacgata ccgaccagat ggacatgcgc acattgtgga acactgacac cgatcgtgct
     7201 cgcgcagaac tgaactggcg tatcacgttg gtattcaccg tgtttatgat ggcacttatg
     7261 gtcgtaccgc tgagcgtggt taacccacgt cagggacgcg tactatcgat gctgccagcc
     7321 atgctgctgt atctactttt cttcctgatc cagacctccc tgaaatcgaa cggcggtaaa
     7381 ggtaagctgg acccgacgct gtggatgtgg accgttaact tgatttatct ggctttggcg
     7441 attgttctca acctttggga caccgtgccg gtccgccgcc tgcgcgccag tttttcgcgt
     7501 aaaggagcgg tgtgatgcaa ccttttggcg tacttgatcg ctatatcggt aaaactattt
     7561 tcaccaccat catgatgacg ctgttcatgc tggtgtcgct gtcgggcatt atcaagtttg
     7621 tcgatcagct gaaaaaagcc gggcagggga gttacgacgc gttaggcgca ggaatgtata
     7681 ccttgctgag cgtgccgaaa gatgtgcaga tcttcttccc gatggcggct ctgcttgggg
     7741 cgttgcttgg tcttgggatg ctggcgcagc gcagcgaact ggtggtgatg caggcttctg
     7801 gttttacccg tatgcaggtg gcgctgtcgg tgatgaaaac cgccattccg ctggtcttgt
     7861 tgacgatggc gattggtgaa tgggttgcgc cgcagggcga gcagatggcg cgtaactacc
     7921 gtgcgcaggc gatgtacggc ggctcgttgc tctctaccca gcaaggctta tgggcgaaag
     7981 atggcaacaa cttcgtctac attgagcggg ttaaaggtga cgaagagtta ggtggcatca
     8041 gcatttatgc ctttaacgag aatcgtcgtc tgcaatccgt acgctatgcc gctaccgcga
     8101 agtttgaccc ggaacataaa gtctggcgtc tgtcgcaggt tgatgaatct gatttgacca
     8161 atccgaaaca gattaccggt tcgcagacgg tgagcggcac ctggaaaacc aacctcacgc
     8221 cggacaaact gggcgtggtg gcgctggacc cggatgcact ctccatcagt ggtttgcaca
     8281 actacgtgaa gtatctgaag tcgagcggtc aggatgccgg acgttatcag ctcaacatgt
     8341 ggagcaaaat cttccagccg ctatccgtgg cggtgatgat gttgatggcg ctgtcgttca
     8401 tctttggccc actgcgtagc gtaccgatgg gtgtgcgtgt ggtcaccggt atcagcttcg
     8461 gttttgtctt ctacgtactg gaccagatct tcggcccgct gacgttggtt tacggcatcc
     8521 cgccgatcat tggcgcactg ttgccaagcg ccagcttctt cttaatcagc ctgtggctgt
     8581 taatgagaaa atcgtaaccg aatgaacgaa taaagggagc gaaagctccc tttattattg
     8641 ttagcatagt gtgcttcgtt cattcctgaa aaatcattaa aattcaatat attgcagtgg
     8701 tgtaggcata ataagacgcg ccagcgtcgc atcaggcaaa gacaaatctc gcctgactta
     8761 ccttcttctc cccccaagca aactccccag cactccgcgt acaatctgat tcgtcacctg
     8821 gcgagcggcg cttttggcca ttgtttgcac cacaccatct ttcttcccgc cgcgtggtcc
     8881 ggtagtgcca aacaaaatat ccttcaatcc accaagaatg ccgtcatcca ccgccacctc
     8941 tttccctttc acggcaggat tattttgctg ctcggtgctt gcctgtacgc ctttttgcaa
     9001 catctcatag gcggattccc ggtccacgtc atcctcgtac ttgccataca ccggagagtg
     9061 attaatcaag ccattacgct catcttctgt caccggcccc atccgtgaac aaggcgcgat
     9121 caccatcgca cgctccacca cagaaggact tcctttcgca tccagaaaag agattaacgc
     9181 ctcgccggtc cccagttcct ggattgcctt ttccgtatca aatgcagggt tggggcgcat
     9241 ggtttgcgcc gcagctttta ccgctttctg atctttgggc gtaaaagccc gcaaagcgtg
     9301 ttgaacgcga ttaccgagct ggccgagcac attatctgga atatcagacg ggttttgcga
     9361 aacgaaccag acgcccacgc cttttgagcg aataagcctt atcacctgct caatcttatc
     9421 cagcagtacc tgcggggcat cgttaaacag cagatgtgcc tcgtcgaaga aaaacaccag
     9481 ttttggtttc tccagatcgc ctgcttccgg caattgttca tacaactctg aaagcatcca
     9541 caacaggctg gcggcgtaca gtttcggcat ctgataaagc ttctcggcgc tgaggatatt
     9601 gataacgcct ttaccgttgg catcggtgcg catccagtct ttgatatcca gcatcggctc
     9661 accaaagaaa tgcgccgcac cttgttgctc cagcgacagc aatccgcgct ggatggcacc
     9721 aaccgatgcg ctactgatat ttccgtactg attctggaag gatttggcgt tatcgccgat
     9781 gtactgggta atcgcccgca aatctttaaa gtcgagcagt aacagcccct gatcgtcagc
     9841 aatgcggaag atgatattca gcacgccaga ttgcacatcg ttgagattca acagccgcgc
     9901 cagcaacagc ggccccagat ccgaaaccgt cgcccgcacc ggatggccct tctcgccaaa
     9961 gatatcccaa accaccaccg gattggcatg cggttgccag tcattgacgc cgatattttt
    10021 aagccttgcg agcagttttt ccgacgccgt tcctgcctgc gcgataccgg tcagatcgcc
    10081 tttcacatca gccataaaca ccggcacgcc gatttccgac aatgactctg ccagtttttg
    10141 cagcgtaacg gttttacctg tccccgttgc gccagtaatc agtccgtgac ggttagccat
    10201 agcgggtagt aaaaacagtt ctgtgtctgg cgtgcgggca attaacaggg gttcgctcat
    10261 gagatatcct ccagtttcct gcctggagta taggcaacgt ccggcaaaga gaatacgtgt
    10321 aatgaagaga aaataaccta aagcgtgttg ccgtgataaa tctgataccc gaggtcaaca
    10381 gtgttgtggt tgtgatcgtt atttttaatt ttgctcagca acatttgggc agccgtccga
    10441 ccaatatcaa aacggggagt aattacgctg gcgaggcttg ggatcatttg cctgccaatc
    10501 tccaacccat gaaaacccgc aatggaaatt tgttctggca cggcgaggtt tcgctcgcgg
    10561 cacagcagaa gcgcacccat cgcaatatcg tcattggtgc aaaatacacc gtctaaatcc
    10621 gggtttgcgc taagggcatc acgcattaac tgcattccta aatgaatgga tgagatggcg
    10681 cgtggattca tgcgtaatgg ggaaagatta tgcagcatca tcgcatcgca atacccctga
    10741 taacgctgtt catcgcgggt gtcatctttg gaaccgagat acagaatttt gtgcctgact
    10801 cgcttctcca gcatggtaca caccatgtca aaagcggcct gccgattatc aaaaccgacc
    10861 tccatatcca gccgttctcc ctgtacatcc atcaattcca cgaccggaat ggtggctgaa
    10921 cgcagaaatt tcaccgtcct gatggtgtga tatttttccg aaagtataat cccgtcaatg
    10981 ttataagaga gcaggttaat caccgactct tcttcggaat cgcgatcgta attgtaatta
    11041 gcaataaggg tctgataatt atgctcagag gtgacggatt ctattcccgc gagaatgtca
    11101 gcgaagagtt gattttgaaa tgacggaatc aagataccga gggtataact ttgtgcattc
    11161 aacaacatgc cgggagcgcg attagggata taattgatct cttccataat tttggcgatg
    11221 cgctcgcctg tttcctttgc cacctttttc ggcgagcgga tataacgact cacggtcatt
    11281 tttgttacgc cagccagcgt agcgatatcc tgtaaagaaa ttctgtgatt gcgcataact
    11341 tctcccaacg tcgggggaat cttcaatctt cccccgaaac tacatcacaa tattttattc
    11401 tttcagtgca atacggcgtt aatggctaac acgcccagca aacccataat agaaataagg
    11461 gtttccatca ccgtccaggt acgcaacgtt tcaccaaccg tcagattaaa atacccttta
    11521 aatagccaga agccagggtc gtttacgtgt gacgcgatca cactgcccgc accggttgcc
    11581 agtaccatta aagcgggatc ggcatgggta acgttgataa tcggcaacac cacacccgcg
    11641 gtggtaatgg ccgcgacggt ggcagagccc agagcgatac gcaacagcgc cgcaacagtc
    11701 cagcacatca ataacggcga aagcgtagtt ccggtcatta agtgtgaaat atactgcccg
    11761 acaccgctat ctactaatac ctgcttaaac gcgccgccgc cagcgataat aaacacaatc
    11821 atcgcgatag cgcctataga atccccaatg atatccatga tttgctcgat ggtgcgtcca
    11881 ttacgtcggc ccagtgtgaa aatcgcaata acaatggcaa taaacagcgc aacggcaggg
    11941 ttaccgacaa attcaaagaa gaggcgcacg gtgttagttt tcggtaacgt aatttcacaa
    12001 acggcggcga tagccatcag gatgaccggg atcacggcag cgaaaatact gttccagaag
    12061 gagggcatct cctcttcgct aaacagatga ggattaaata agccttccgg tggtgctttc
    12121 tcaaagcgag ttagcagttt agaaaacagc ggtcctgcga caataactgt cggaatggta
    12181 atgataaatc catacagtaa agtcgttccg agattagcct caaagatagt cgcgatggca
    12241 gtaggccctg gatgtggcgg cagaaaacag tgggttacag agagcgctgc taccattggt
    12301 acgccaacat acaacagggg taatcctgat gatgctacga tggtaaatac caacggcaac
    12361 agcaggacaa aacccacttc aaaaaacatg gcgaggccca caaccagacc ggtgatcact
    12421 agcgcccatt gcacgcgttt tttaccaaaa gtagcaatca gcgtagtggc gatacgttgt
    12481 gccgcacccg tatcagaaat cagcttgcct aacatggccc cgaaaccgag gatcattgcc
    12541 agcccgccga gcgtgctgcc gataccattt tgtatagagt gcaggacggc ctgtgcatcc
    12601 atcccttcgg caaatccgac gacggcagct accagaacga gggcaataaa gccgttaact
    12661 ttaaagccga tcatcaggat aagaagcagc gcgacgcctg ccgcaataat gattaatggc
    12721 atagtcttta tctcttatta gtgccagaaa agcagcgctt tcgcgctgca cgtagggggg
    12781 gctgttaaac agccactaac atgccgccat caacaaacaa caggtggccg tttacgaaat
    12841 cagaggcttt tgaagaaagg aacaccgcag caccaatcag ttcctgcgga tctccccagc
    12901 gtgcggcggg ggtccgtttg cacaaccagg cggtgaaggc ttcgtcctca accagtgctt
    12961 tagtcatttc tgttttgaaa tagcccggcg caataccgtt gacctgaata ttgtggcgcg
    13021 ccagctcgac gcacatgccg cgggtgagca tttttaccgc ccctttcgat gcggcataag
    13081 gggtgatggt gtcacgtccc agttcgcttt gcatcgagca aatattaata actttacctg
    13141 ccttgcgttc aaccatgtga cgagttaccg cttgcgatac caggaacacg gcggtctggt
    13201 ttactgcgat cacatcattc cactcttgtt cagggaactc agtaaaagga tgacggcgct
    13261 ggataccggc gttattcacc agcacatcaa tggggccgat gtccttttcg atatgttcaa
    13321 cggcggcatc aatttcatgt ttatgagtaa cattaaaagg tgcggcaacg gcctgaatac
    13381 cctcctggtg gagtttttct acagcaagtt ctgcgcgttc ggcagtaata tcattaataa
    13441 ttatttgtgc gccatattta cccaggccgg ttgccagtaa aaagccaatg ccctgtgctg
    13501 aaccggtaat caagatattt tttcctgcca gtgaaaatag atcgttcatt gtattattcc
    13561 tttatttatc tcttagaaaa caagctggac ttttgctgcc tgggttttat caccggcgaa
    13621 acgtagcgcc tcttccaggt cagtgaaggg atattcagca ctcagtaaag gcagtggatt
    13681 gataacgcca ttcgccagcc atgacactgc ggtattaaat tcgctggtaa aacggaaaga
    13741 gcctctgagt gaaatctcct taccaatcaa cgtcatcatt gggaattctg ccatcgcgcc
    13801 tcccatacct acctgcacca ttacgccgcg tgcacgagtg acctccagac aggtattcac
    13861 tgatgaagga tgaccggaca cttcaaagct gacatcgaaa tagccttttt ccgctttcca
    13921 gtgatccatg tcgtcgtttt gtgggtttac gagcacatcc gcccccatct ctttgcccag
    13981 cgaaagggaa cggggactca catcagcaca gacaatttcc gcggccccca gtgttttcac
    14041 tgcactgaca atcaggcagc caatgggtcc aacaccggaa ataaatactc gcttgccctg
    14101 taactcgccg gcctgatgtg cggcatgaat cgcgacggct aaaggttcgg caaaagccat
    14161 aaccttttcg tcagctttgg ccggataagg gacacattgc gacgtttcga ccattttata
    14221 acgggtaaaa ccaccatcaa catgagggaa atacatggca ctgccaaaaa aacgcatatc
    14281 tgtacactga ttctcgttat gttcaatgca gtatttgcag tgaccgcacg gtttagacgg
    14341 attaatggct accgtttgcc cttcatgtaa ttctgatgag tcgctatgaa taactttacc
    14401 gataacttca tgacctaaca ccatcggtgc ctttatcatg aaattaccta cttttccttc
    14461 ctgataataa tgtaaatcgg aaccgcaaat tccacctcgg gttatttgta ctaatgttcc
    14521 attattattc caatctatcg tctgctcggt aacggcaaca gttttcttgc ccgcaacaac
    14581 gcaggactgt gttttcactt gcataaattc ctcactggtc aggtagacac ctcggaagca
    14641 tttaagcggt tttaattgtc attatttgtg atgaagatca cgtcagaaaa ttgttacatt
    14701 actatgttac gcataacgtg atgtgccttg taattcttat cagcagaaat aaaaaaacgt
    14761 gaaattatta tgccaccagg cgtagtatcg cagcaggtaa gatgattcag gagattttaa
    14821 atggcgggtg aaagctttat tttgatgggc gtttcaggga gtggtaaaac attaattggt
    14881 agcaaggttg ccgcgttatt atctgctaaa tttattgatg gtgacgatct tcatccagcc
    14941 aaaaatatag ataaaatgtc gcagggtatt ccattatctg atgaagatcg acttccctgg
    15001 ctggaacgct taaatgatgc ttcatacagt ctttataaaa agaatgaaac aggatttatt
    15061 gtctgttcat cattaaaaaa acagtatcgt gatattttac gcaagggtag cccccatgtt
    15121 catttcctct ggttagatgg cgactatgaa actattctcg cgcgaatgca gcgtcgggct
    15181 gggcatttta tgccggtagc gttactaaaa agtcagtttg aggcactgga gcgtccacaa
    15241 gaagatgaac aggatattgt gcgcattgat atcaaccatg atattgccaa tgtcactgaa
    15301 caatgtcggc aggctgtgct ggcgatacga caaaacagaa tatgtgcgaa agagggcagc
    15361 gcctcagatc agcgctgcga atgattttca aaaatcggct ttcaacacca cgcggtaacg
    15421 cgccttaccg tcgcgcacat gctggatggc gtcgttaatt ttcgacatcg ggaacaattc
    15481 ggtagtcggc gcaaccttgc tgcgggcggc aaaacgcatc agcttacgca actcataagg
    15541 cgtgccagta gcagagccag agacactgcg atcgcccgca attaacgtaa aggccggaac
    15601 agacagtggc gtgagaaccg caccgaccgt atggaaatta ccgccatacg tcagcgcctc
    15661 aaaataaggc tgccagtcga ggctgacgtt cacagtattg ataatgagat caaactgccc
    15721 cgccagagtt ttcagtgcct gcggatcgcg gctattcacc actttatcgg cacccatcgc
    15781 cagtacttcc tgctctttcg ccggatttga actaaaggct gtcacctcgc atcccattgc
    15841 gtgcagaagt tttatagcga tatgccccag cccgccaata ccaattaccc caacgcggct
    15901 ggtagcagtg atatggtgca tcaatagtgg tttaaagacc gtgataccgc cgcacaacag
    15961 cggcccggcg gactcgatat caatattttc tggcagtgga atcacccatt gccagtccgc
    16021 acgcaacttc tcggcaaagc caccgcgatt cataatcgtc ggcaccgcac cttgctcgca
    16081 gttgatctga ttaccgctaa tacaggcgtc gcagtgacca cagctacgcg ccgtccagcc
    16141 aatcccgaca cgctgaccga cctgcaaacc tttatcctgc gcggcgctcc cgagtgccac
    16201 cacgcgccca atcacctcat gcccggcaac cagcggatac tgtgaaaatc cccattcgtt
    16261 atcgatcatc gacagatcgg aatggcagat cccgcagtaa tccacctgca cttcaatatc
    16321 ttgtggcttc agctcaccgg gatcgtactc ataaacttcc agttcgccgc ccgcttcttt
    16381 tgcggcatag ctttttatca tcgacatttt ttggtccttc tctggtgttg tctaggaagt
    16441 gtagagcatg gcagggcggg gtgcctggag tgtgacaaag gttacacatc gcggtatgca
    16501 atgctgaaaa tttcagcact tagcgaggtg cgagcaagct ggcgcttgca tggtggtgcg
    16561 tgacaggtat aattcacaac gttttccgca tacctcttca gtgccgaagt ggcgaaatcg
    16621 gtagacgcag ttgattcaaa atcaaccgta gaaatacgtg ccggttcgag tccggccttc
    16681 ggcaccaaaa gcatgtaaat ggacctcaac tgaggtcttt ttttatgcct gaaatccccg
    16741 tcatacaagg ccttccgcga ttttcctttc aacagaagtc aatctgagtc aacccacatc
    16801 aagtcgcttg tgagtataga accgagtata tatgctggtt cgataatggt tgtatactca
    16861 cagcggcaca tggaaggaga acttttatgg ctctgacaga tactaaagta cgttctgcga
    16921 aaccgcagga aaaggagtac accctagtcg atggggatgg catgtttttg ctgattcatc
    16981 ccaacggctc aaaatactgg cggttccgct ttcgcttcgg tggcaagcaa cacctgatgg
    17041 ctttcggtgt ttaccctgaa acttcactgg ctgatgcccg tcaaaaaaga gaagaggcta
    17101 ggagacttgt tgctgctgga atcgaccctc gcgagcataa acgtgctgtg aaagaagaac
    17161 aggcgaaaga ggctattacc ttcgagtcag ttgccagaga atggcacgct actaataaga
    17221 aatggtccga agaacacagc cgaagggtat tgaagagctt agaggacaat ttgttctcgg
    17281 caattggcag acgaagtatc gaagagctaa aaactcgtga cctgcttgca cccataaaaa
    17341 tcgtggaagc gacaggccgc tttgaagttg cttctcgcct tcagcagaga accacagcaa
    17401 tcatgcgcta tgcggtacaa agtgggctga ttgattacaa cccggctcaa gagatggctg
    17461 gcgcagttgc ttccagtaac cgtgtccatc gccctgcact tgagctgaaa cgtctacctg
    17521 aactgctcca ccgaattgac ggttacacgg gacgcccttt gacccgcctg gctgttgagc
    17581 taaccttatt aatcttcatt cgttccagtg aattgcgctt tgcccgctgg tctgagatcg
    17641 atttcgatac ggcaatgtgg acgataccgg cagaacgaga agctatcgaa ggagtgaagc
    17701 actctcaacg tggttctaaa atgcgcacac ctcatctggt gccattgtct catcaggctc
    17761 tggcaatcct caaacaggtt cacaagctta gtggcgagcg cgattttgtt ttcattggcg
    17821 atcatgatcc gcgtaaaccg atgagtgaaa acacggtgaa caaggcgctg cgcgttatgg
    17881 gttatgacac tagggtagag gtttgtgggc atggtttcag gactatggca tgtagctcgc
    17941 tgattgagtc agggctgtgg tcgagggatg cggtagagcg tcagatgagc catatggaac
    18001 gtaactccgt tcgagcggct tatatccata aggcagaaca tttggatgag agaagattga
    18061 tgttgcagtg gtgggcggat tttctagatg taaatcgcga gaaagtgatt agcccgtttg
    18121 attttgccaa aatttttgcg tcgttaaagc gctaaagctg tgtaacaaaa atagagccta
    18181 ccgtcacact ttgttaagta ggctcgttgg tcaaagttgc aacaatttca tgatggtagc
    18241 tgtccgcgca ttggtttcag gtagttctgc agagtattga acgtacctgg agaaaaaggc
    18301 atatggtgct caattgttag cttgtataga ataaggatat tctttcttta gagaacatga
    18361 agttatgagc gcgatcacat tacgtaaagc gttaggtgta cttgcgaagt catcttcgtt
    18421 ttccgtaacc acagaaactc atcggcaaaa ggatgtgttc gatgagctaa aagaacaact
    18481 ctttgttaaa caagagattg aagaagagtt acagcgttat ctggatacag ctactcccgg
    18541 tgagatcatt tttctatgcg gaagtagtgg agacgggaaa tccgaaattt taacccgctg
    18601 taagtcagat ccacaatatc aacagaagtt tagtttccat ctggatgcga cacatagctt
    18661 tgcgcctcaa caatcggcta tcgatgcact gaatgattta tttgaaaatc atcattcgca
    18721 atcatacccg ctcctcatcg gtatcaacac cgggatgcta gccaattttg ccagagaagg
    18781 ttctgagtcc catatggcta tccgtgctgc tattgactca ttcttgtcag cgcaacagga
    18841 aggccagcgt ccttaccgca acgaaaattg tatatttttt gatttcgaac attatccaaa
    18901 gttcgagttt gacgaagagc gggaatactc cccttttatc aaaaaattaa tgaatcaatt
    18961 aacggggaat gacgacaaaa acctgtttta cacaatttat caacgtgatg aatcaatagg
    19021 acatgattta aaaaccgttg ctaactttaa actactgtgc atgcccggtg ttcagaatgt
    19081 tttaataaca cagctattta aagcccgatt aataaaagat caatttgtta ccacaagaac
    19141 cttattggat tttttacacc acctgctgac agggcctggg tacctgtttg ataatctgtt
    19201 taatggtgca gagaatgatc taattaaaaa ggtttctgat tttgatcctg ccagattgca
    19261 tacatacgag attgatcaat ttattttgcg ctatgagctt ggattagttg atcccgaact
    19321 ggatgatttt ctgagtacac ttgcgaaatt acatcttacg tttgatcgtc aatgtgttaa
    19381 gcctggtgac gcagcttcat taattagact tttctggcta ctacaacatg aatctttagg
    19441 gaataactat cacaaaaggt tctctgtatt ctttaaagaa gcgctctttg agcgttattc
    19501 agagatttgg catctgcata aaaactatac cgcagatccg gaacaaaaaa gaacactaaa
    19561 ccgcttttac tctttcgagc ttatcgctgg tattcagcgt tacgcaaacc gtaaagcacc
    19621 tgaactgagt acccaaaaag aagagttctt tttaggcgaa tttggtggga ttaaaatcac
    19681 agcacctgtt gcgctgaaac cggactggga ttcaattctc aagaaaaata cagcccatac
    19741 aacttgtttc gatatctatt taaaagtagg acaagatccg ctagatccga ttcggatcgg
    19801 tcttaattta tttgagctat tgagcaaact aaataatggc tatcgcccta ataagtatga
    19861 taaaagtgcg attgtcttac tggacgaaat cgttgaacta atcgcagaaa aggcaaaatc
    19921 cagcagcgaa atcaagtttt atgatggcct aaagcgggtc tatagtgcaa gagcggacga
    19981 tgacatgatt actatcagcg gcatggaggg ataacatcat gtatcccatt gcaacaaatt
    20041 taaaagttag taataaccag ctggatagct accttccaat acgtaataag aataacaata
    20101 ttgactggca aatcgtcaca ggtttagtac tgagctatgc cgtgaagtat aaaatcgata
    20161 catacagtct cgagcagttt cgcgaggatt gtaaaactca tctgcaaata ttgatcgatg
    20221 aacctgcgtt cttgtccgtg ctggaacgga tgtatttttc cagtcaggat atttttcgtg
    20281 tctccccgct gttcttgctg ttccacgctc agtttgatgg tgaaaagata agtgcaggct
    20341 cgactgccga caaacgcctt ggaacactgt ttgccaattt gatgagagat tttagcctta
    20401 ataacccgat acaagataag ttgaatttta ttgaaaaaga gatgctgaat aagctcaata
    20461 aaaaattaat ccgactgggt gaggggccat ttgcaaaaga acaaccttat ttaccctatc
    20521 tggtaacgtg ttttcagtct gatttggcgt ttctggcaga gcacccacaa tatcttttgc
    20581 aggagctgac caatactcta cgtttgtatg cctttagttg gtgcgcacaa ctggcgctca
    20641 atctggacaa ctggcaagac ggcgagccgc agagtaaatc attgttcttt attctcgata
    20701 ctgaaaaagc tagctccgaa cgagataaaa taaagctctt cggctataag tggtttgccc
    20761 gccaaagtga aaaattattc cccgtactct ctgcattaga ggtattacaa gtaaaaggtg
    20821 aggaaaagcg ccctctttgg caggtatatc aagattgctt aggttattca gatacatcga
    20881 atcgagtact aaatgaactc aataattata tacagaagtt cattagtaag gaggagcgag
    20941 accttcctga acgcgatcgt gctacaaacc tggaagatgc ctttaaacag ctgttatctg
    21001 ttgccgttga acagtttcag ggtaagaaaa ccgagcgagc agcggtaaat cgcaaataca
    21061 tcaacgagct ggaaagccag atttgcactg acttcattca ggttcgtggc cgagcgggga
    21121 aagtgctggt actgaaccag gatcgactac tgctactaac taaccttacc gtgggtaaaa
    21181 acaaaaaact ccgtctgcat gagctgctac gtggttttga gcaacgcgga ttttatctgg
    21241 ataaccagtc tacacaaatg ctggttgcgt tttatgaacg catgggaaat gtggagagaa
    21301 tgagtgacag tggagacgcc gtatatgtcc gtaaaacagt atgaaaccta tctggcagaa
    21361 accttcattg aatgggttag cagcatcatc cagcctggtg agcgctacca gtttaaatct
    21421 cccgatccgg ataacgcctt aaagctctgg aaggctttcg actctttagc aaatggaaat
    21481 aaactagaaa ttgcaccgga gcaatttctt acctacgtcc aatgtaataa tatccagctt
    21541 attccggtac tgcacggcac cactacccct gcattcacag aaaactatat ttctcattta
    21601 cgcgatcagg tttctggccg caatggcatt tttgcccaga cagcgttgtt gattatccac
    21661 aacagcatgc tggatacctt actcaatagt accaaagatg tcgctgcacc ggatgctatc
    21721 tggcatccag aaaccttccg tcaccagctc gaaaagttaa tcacaacgga cagcgcccgt
    21781 tctcaagtat cccgctgttt gctgggcgat cagttgacaa caatattgga tgaaggcgct
    21841 acggtttttg gattttcatc gttataccgt ctgctggacg acggcaatct ggatttctca
    21901 gagctaaagt tattcaacga taataacctg ctgaatttta gtgataagca gttgcgcgcc
    21961 cggctcaatg aaaaccaaaa gttatatcgc cagatagagg acagtgttga acgctacagc
    22021 gggcaactgg agaatgtact aactgaattt agtgcaaaat tcattcagga gcatttcgtt
    22081 gacaatgacg attggcgtga gcttgatttc tccgtctacc tgaatgaaaa agcacaaaac
    22141 agtgaacaga aactggtgtt ggaaaacatt actgctgaaa acggtgaggt ctggcaaaga
    22201 gccaaaagca ccagtaaagc aggtaaacga gatatcagtg tcctggttca ggttctaccg
    22261 gggcagtcac aagttgagct ggagtttatc ttccagggta acgaccttca ggacaatcag
    22321 gtaaaaattg ctcatcatcg tccgttacag aaagaacgat tctggcgtac tagccggctc
    22381 ggggggagaa actctaagat actggcatca gtgccttttg atggtcggcc ctgcttcttt
    22441 agcctcgaaa tcattaaccg caataactca gcagaagagt acaagtttcg cctgctgtta
    22501 gttgaacaag ggcaattctg gcttaatgaa atccagcatt gttatcgtat tgaacctggt
    22561 aaaggtcaat taactctgca actggaagat aatgagttac gtattgcaga gttcggcgac
    22621 aagacctgtt tacttgatga agaaagtgat gatgttgatt gccgccatta tgcactcgtt
    22681 aattttgaaa cgctggcgaa tcaaagtgag ctgatcaaat ttaccttgat ttcaggtgac
    22741 tcccgtttac tgtttaacat cgaaggtcca ggagcagagg aaggattaac actgcctctg
    22801 ctttttgatc agggtcgttt caataaactc tttaaagaag aagggaatgc aacctggaac
    22861 aagttgaaag gtcgcgtcat cctggacaat atcgaacata atgtggttgg tgttcgccag
    22921 cagttgctta tgcaagagta cgccctgatc gagcgtaatc tgctcagcac tggcagtaat
    22981 ggtagtgagt ttgcactgga tgagcttctg gcgagctatc ccgacctcca taatgcgtac
    23041 caccaattgt tcgtttacta tcaacgtcga aataccctgc cgagtctggt gtcatggtca
    23101 gcagaatatc gtgtattagt cagtcacatg atcgctactt ttgaacaggc attacagcaa
    23161 atcagtctaa gcagagcatt aaccatccag gaaaaacgtc tgttacatct ggggatttgc
    23221 cgcggcgacg attatgaacg tctctcgcct ctgcacccat tggtaatggc ttatcattta
    23281 cagctagctg aaactattat cgccgagccc ggatacccca catcagcttc cttcgcttca
    23341 ttaccagaga ttactctaga tcgtctggtc gtctctggcc taatgccatt tgtgtatcat
    23401 agcgaacatg aatacgcaca gttacaaccg atggtagaaa accgcttctg gatagatatt
    23461 gttccccagc gccagatgag ccacgaatat gttaagcgtc tggtgaaaga caagctaaac
    23521 gaatttacag atgcctattc tcggctgttc cagagtgcag gaaataatgc cctggttatt
    23581 aatgctatta atatgggaga agccagagag ctattcctgg gactggtaga ttattttaag
    23641 caggaaaaag ataacgcaat ttcgatacac gtaaactgct atgacgaacg tctgctaccc
    23701 aatgcctttg accgctttgc cgaaagcggc agctatgaac aacttaaaaa tgatcttggt
    23761 ctgaacagcg gtgcgtggcg ttctgaagcc gatatgttga tcgatctgct tcgcagtcgc
    23821 ttaacattca gcaaatttat cctgccaccg gaaagcgatc aactggctta cgcccatctc
    23881 gcatttttca ccaacaccgc accggttgat tgccgccaaa ttcgcattga ggatgctgcc
    23941 agcggggttt tatgtcatgg cttgattgca ggcgaagggg ctgaaacaca aggcgatgct
    24001 tactttaccg cgtttggtct gcgtaatgtc gatactgaat cgtcttgcgc cctgcgtctc
    24061 gcccgcttgt taggctgtct gtggcaaccg gcacggcaga gcaacagcca gtatcacggt
    24121 cagggaatta gtctggctgt gagcggtaac tttaagcaac tgctgaatcg ctcttatgat
    24181 agctctctct ggaccacaat tatcgaccct aaggtcacac tcgatttctt tactagtcaa
    24241 aaagatgtcg tactaattca ttactctgac cagtacacca gttgtgctgg ttatgacgcc
    24301 gtaactgtca caaaacaggt tgagttgttc ctgcgtctcc ttcaaacagg acaatccacc
    24361 gttgatagcc agcaactact tgctgaattt aatgccttta acggtgagtg gctgttaaaa
    24421 atgttgcgtt caaatgaaaa ggagcgcaaa gaaaaacacg gcattatcgg tgcatacaaa
    24481 ttcgtacagt ctatgctgct ccagtcagat atttgctgga ttccactgtc agtagctgag
    24541 atgatccgcg tgtccggcaa cgtcgggctg agaatgaaag aaagcgattt atcgcgccat
    24601 ttacagggtt atcagaaagg agcgatctct gacgatgtgc tctttgtcgg tttcaaagat
    24661 aacaacctgt atctgttacc acttgaagta aaaacaggag ctcgccccga ttacaaccat
    24721 gcggggcagc aggcccgcga gctaaaacgc tatttacagc aagatattct tgggccgaaa
    24781 actctagcat cacaactata ccgggcactt tttatccgcc aagtattaat gcaggtagag
    24841 aagttgcgtc tgtatggtgt actggataac gacaagctat cccctctgct ggaacgacgt
    24901 gaatggtggc tcactggcga ctatcagtta ggtgagcttg ctgattatgc agaaggcttt
    24961 gtggtagctc atgtagacag cggaacctgt tttgatctct cttataaaga aacggcagag
    25021 aatattctac agatcgaaat cccctattca ttgcttccat cgttaattgc agcacaaggt
    25081 gatcaacctc cactagctga acgctatcgg gtgccggaaa aatatctgct caaaccagag
    25141 tgtgatgtca attcttcaca cccagtacag gtagcaacaa caactgagcc gccgcccatt
    25201 tctgcgctgg agcttccaca acctgaagca tcggcactaa cccccagtaa tgtccgcaat
    25261 acaccattac agatcttgtt tggtcatgat gctgtgcgtc agaatccatt gtactgggaa
    25321 ccaaccaaca ccgctaagtt catgaatact aataccggca ttattggaac aatgggcacc
    25381 gggaaaaccc agtttactaa atcgctggtt acgcaactga tgcgcaatca gtcctacaac
    25441 gttgacggta aaccgattgg cttgttgatc ttcgactata aatccgacta tgtggatgat
    25501 gcgtttcttg aagctacggg agctaagagg tatcagctat cgttgttacc ttacaatccg
    25561 ttatcgttat ttggtgatat gcccatgtta ccgaggcata cggccatggc attcgctgaa
    25621 acgatgggca aagcgtataa ccttggcgta aaacaacgca tgaagctggt tacgttaata
    25681 atggagtgct atgacttagc aggaatagtg cctcatgatc gatcaacatg gaatcgtgta
    25741 gcaccgacta ttgaagatgt gtggcagcag tacctggcac aagagaaggt cgacgaagat
    25801 tcattatatg ctgcacttta taatctggct ggtttccaga tttttgaaac ggatccagag
    25861 aagatgacca gtttgtacga tctggttgat ggagtcacag ttatcgaact cgctggttat
    25921 ccatcagaga ttcaaaacct ggtagttgca ctgacactgg atctttttta tgcacagatg
    25981 caaaagcgtg ggaaaccgac ggtgcgaggt gattatcgcc agttaaccaa aatgattctg
    26041 gttgatgaag ccgataactt tatgcgccag gattttgcca gcttgcgtaa aatccttaaa
    26101 gaaggccgtg aatatggtgt aggggcgatt ctatcaacac aagaaattac tcactttaaa
    26161 acaggcgaga ataattatgc ttcttatatt ctcacttggg taattcatcg agtttctgag
    26221 attaggaatt cggacattaa ggcggtattt aacattgacg ataaaagtga gcaggagtct
    26281 ttaatgggcc agatacgaca acttgagaa
//
LOCUS       DAZFRN010000060        26289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFRN010000060 DAZFRN010000000
VERSION     DAZFRN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.060"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKUB_002397"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKUB_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO6655408.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHKUB_002398"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHKUB_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO6655409.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHKUB_002399"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHKUB_002399"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO6655410.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHKUB_002400"
     CDS             complement(1154..1621)
                     /locus_tag="ACHKUB_002400"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO6655411.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWPFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHKUB_002401"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHKUB_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO6655412.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHKUB_002402"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHKUB_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO6655413.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHKUB_002403"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHKUB_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO6655414.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHKUB_002404"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHKUB_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO6655415.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHKUB_002405"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHKUB_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO6655416.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHKUB_002406"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHKUB_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO6655417.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHKUB_002407"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHKUB_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO6655418.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHKUB_002408"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHKUB_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO6655419.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHKUB_002409"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHKUB_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO6655420.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHKUB_002410"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHKUB_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO6655421.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHKUB_002411"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHKUB_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO6655422.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHKUB_002412"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHKUB_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO6655423.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHKUB_002413"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHKUB_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO6655424.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHKUB_002414"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHKUB_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO6655425.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHKUB_002415"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHKUB_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO6655426.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHKUB_002416"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHKUB_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO6655427.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHKUB_002417"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHKUB_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO6655428.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHKUB_002418"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHKUB_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO6655429.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHKUB_002419"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHKUB_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO6655430.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHKUB_002420"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHKUB_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO6655431.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHKUB_002421"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHKUB_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO6655432.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHKUB_002422"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHKUB_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO6655433.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHKUB_002423"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHKUB_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO6655434.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHKUB_002424"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHKUB_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO6655435.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHKUB_002425"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHKUB_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO6655436.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHKUB_002426"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHKUB_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO6655437.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHKUB_002427"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHKUB_002427"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO6655438.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHKUB_002428"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHKUB_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO6655439.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHKUB_002429"
     CDS             15761..16129
                     /locus_tag="ACHKUB_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO6655440.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGGGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHKUB_002430"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHKUB_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO6655441.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHKUB_002431"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHKUB_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO6655442.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHKUB_002432"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHKUB_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO6655443.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHKUB_002433"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHKUB_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO6655444.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHKUB_002434"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHKUB_002434"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO6655445.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHKUB_002435"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHKUB_002435"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO6655446.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHKUB_002436"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHKUB_002436"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO6655447.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22834
                     /gene="dprA"
                     /locus_tag="ACHKUB_002437"
     CDS             21710..22834
                     /gene="dprA"
                     /locus_tag="ACHKUB_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO6655448.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22806..23279
                     /gene="smg"
                     /locus_tag="ACHKUB_002438"
     CDS             22806..23279
                     /gene="smg"
                     /locus_tag="ACHKUB_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO6655449.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23308..23850
                     /locus_tag="ACHKUB_002439"
     CDS             23308..23850
                     /locus_tag="ACHKUB_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO6655450.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHKUB_002440"
     CDS             23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHKUB_002440"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO6655451.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24432..25250
                     /gene="aroE"
                     /locus_tag="ACHKUB_002441"
     CDS             24432..25250
                     /gene="aroE"
                     /locus_tag="ACHKUB_002441"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO6655452.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25247..25504
                     /locus_tag="ACHKUB_002442"
     CDS             25247..25504
                     /locus_tag="ACHKUB_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO6655453.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25480..26034)
                     /locus_tag="ACHKUB_002443"
     CDS             complement(25480..26034)
                     /locus_tag="ACHKUB_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO6655454.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6740 a         6245 c         6822 g         6482 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgccagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 agggccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg gtagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttccgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggca atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaaatct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtggttcacc caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc
    11521 agccagtaca cccgttacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccagg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagattactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaatat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacagc gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagtatcc ccacttatct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgc catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtgg
    15841 cggcgaacca ttgatactgg atgatgattc tcacgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcaacgga gagaagcaat
    15961 tcagcttctg gatattctca aaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acctgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggcgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ctcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactgaca ggattacact cgcgccgtta aataaccaac tggagttttt
    16621 atgagccgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggccttct gctttcaggc gtttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgt cagtaatact tcttctttag ttggtgctgg tgcggctgct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaagtc gatcccgcca
    17101 attaataagc ccagaggtgg catgatgata tcggcaacca gtgaggatac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggctgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacgctc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcacgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttccacaatg gtcgaacctt gtggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcgttac caatcagcgg gccgccataa taggctttaa ccacagcgag gctgacttta
    18301 ccctcagcga agttcaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtgatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggcag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
    18901 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgctttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc cagaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccaa caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttggcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcattaaact cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgttt atcaaccctt taagttgcgg acgcttaatt
    19741 gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca aaccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgcgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaacgcccg gcaagaccgg gcttagaaga
    20101 gtggacaatc agaccagacg gttgcccgga acaaaccatt cccgacgaga gttcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgtttg ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgatgctttc cagactttaa ccggctgtcc ttcaatttcc
    20341 agccagctca ttggccatgg attgaaagcg cgaatgcagc gttcaagctg tgctgccgaa
    20401 agtgaccagt caatacgcgc ttcttcttta ctcaactttt cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgccagtt gttttaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata gagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcgccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 acagctttag gcagaattaa accataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggttttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc tgacgaatac
    21121 gttgttgttt caacggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagaccgt ctgcttccag ttcaaatggt ttaccgtcgc
    21241 ggtccagggc gcgaatttta actttctctg cgcgtggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc gtacatcgtc tcgaacatat
    21481 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agaaatttgg ctgcgtttaa tgagtatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcaaat tgatgcggtt ggattgcagc aaacagggct
    21841 tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact
    21901 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
    21961 tcaacttctg gtgacgacag attacccagg cgcactgttt gttgaaggag aactgcacgc
    22021 gctgcattca tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg
    22081 atggggacgg ttattttgcg aaactctggc gacgcgtgga gtgacaatta cgagtggact
    22141 ggcgcgtgga atcgatggtg tggcgcataa agctgcctta caggtaaatg gcgtcagcat
    22201 tgctgtattg gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc
    22261 cagtttgctt gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct
    22321 tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt
    22381 ggtggaagcg gctttgcgca gtggttcgct agtgacagca cgttgtgcgc ttgagcaggg
    22441 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
    22501 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
    22561 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
    22621 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
    22681 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
    22741 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
    22801 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt
    22861 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag
    22921 atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag
    22981 aaccgatgca actggcctct gatccactct ccatgcgtat ttatacccct gaggagtgtg
    23041 aaagactgga tgccagctgt cgtggttttc tgcttttcct tgagcagatt caggtgctca
    23101 accttgaaac tcgtgaaatg gtgatagagc gagtgctcgc gctggataac gcagagttcg
    23161 aactggatga tctgaaatgg gtaatcctga tggtgttgtt caatattccg ggctgcgaaa
    23221 atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat
    23281 catttcttta attcagcata agttgttatg gcgaaatcag cactgttcac ggtgcgtaat
    23341 aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa acacggtccg
    23401 tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg
    23461 gatggacata ttgtcaaagt tctggagggg caggtttgcc ctgcatgtgg cgcaaatctg
    23521 gtattacgcc agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa
    23581 cataccgaac ttatcgataa accggacgaa acagcaatta catgccccca atgccggacg
    23641 ggccatctgg tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac
    23701 ccggagtgtc aatttgccat taacttcaaa cccatagctg gagaatgccc tgagtgtcat
    23761 tatccactac tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa
    23821 caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc
    23881 tatcgcagct gcgatagatg tcctcaatga agaacgtgtc atcgcctatc caacggaagc
    23941 cgttttcggt gtcgggtgcg atcctgatag cgaaacagca gtgatgcgac tgttggagtt
    24001 aaaacagcgt ccggttgata aggggctgat tttaatcgcg gcaaattacg agcagcttaa
    24061 accctatatt gatgacacca tgctgactga tgcgcagcgt gaaaccattt tttcccgctg
    24121 gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg
    24181 ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca
    24241 ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg
    24301 aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac
    24361 gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca
    24421 ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc
    24481 cattcattca tcagcaattt gctcagcaac tgaatattga acatccctat gggcgcgtgt
    24541 tggcacccat caatgatttc atcaacacac tgaacgcttt ctttcgtgct ggtggtaaag
    24601 gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc cagagcggat gagcttactg
    24661 aacgggcagc gttggctggt gctgttaata ccctcaagcg gttagaagat ggacgcctgc
    24721 tgggtgacaa taccgatggt ataggtttgt taagcgatct ggaacgtctg tcttttatcc
    24781 gtcctggttt acgtattctg ctcatcggcg ctggtggagc atctcgcggc gtactactgc
    24841 cacttctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag
    24901 agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg
    24961 aaggtcatga gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc
    25021 cggcgatccc gtcatcgctc attcatccag gcatttattg ctatgacatg ttctatcaga
    25081 aaggaaaaac tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg
    25141 gtttaggaat gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc
    25201 ctgacgtaga accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat
    25261 ccagtttccg gacagagaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct
    25321 cgtgaatggc atgcaactga catgtgcgat ctctggcgag agtctggcgt atcgttttac
    25381 tggagatacg ccagaacagt ggttagcgag ttttcgtcag catcgctggg atctggaaga
    25441 agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc
    25501 ctgatccaga tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc
    25561 cttctcttca tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc
    25621 gctctccagc cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc
    25681 tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt
    25741 gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt
    25801 gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt
    25861 ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat
    25921 ccccacatca tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac
    25981 gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac
    26041 ctcagcaata aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt
    26101 tagtatgcaa aaaagcaccg ttttgtgtgc gaatgcagca aaaagggtga aaaaacaaca
    26161 aacagaaaaa aagatcaaaa aagtacttgt gcaaaaaatt gggatcccta taatgcgcct
    26221 ccgttgagac gacaacgtga aacacttcac aagatggtcg taacaacgaa gagaaaaaat
    26281 cctgaaatt
//
LOCUS       DAZFRN010000061        26184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFRN010000061 DAZFRN010000000
VERSION     DAZFRN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.061"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..816)
                     /locus_tag="ACHKUB_002444"
     CDS             complement(<1..816)
                     /locus_tag="ACHKUB_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011146066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFO6655455.1"
                     /translation="MKIFISDLQKAELERLHDTSRNKRVCDRIKAILLASEGWSSVMI
                     AQALRLHQTTVDHHIHEFMNKGKLKPENGGSDSKLSAEQTSLLIKHLSDNLFHHTHEI
                     IAFVLHTWGILFSVAGMNKWLHRNGFSYKKPAGTPHKFSEERQAQFIEYYEDLKEKAG
                     DEPVLFIDAVHPTQATKISYGWIRKGHRKAIETTGSRTRLNIMGALNLKSPERPLISE
                     YPTINALSISRFFNEIRKVYPDYNQTVNIILDGARYHHAQLVTDWAEVVNIKRV"
     gene            1206..1406
                     /locus_tag="ACHKUB_002445"
     CDS             1206..1406
                     /locus_tag="ACHKUB_002445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4695 family integrating conjugative element
                     protein"
                     /protein_id="HFO6655456.1"
                     /translation="MSEHLEILKSLNTLGLVVNVESVERMEVLQKRADGLLLLPVICD
                     NFVQMLQLNAYPVLITEMEISQ"
     gene            1432..1626
                     /locus_tag="ACHKUB_002446"
                     /pseudo
     CDS             1432..1626
                     /locus_tag="ACHKUB_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(1678..2448)
                     /gene="yafV"
                     /locus_tag="ACHKUB_002447"
     CDS             complement(1678..2448)
                     /gene="yafV"
                     /locus_tag="ACHKUB_002447"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO6655457.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQYNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQTYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYREKFPAWRDADEFRLL"
     gene            2629..3075
                     /gene="ivy"
                     /locus_tag="ACHKUB_002448"
     CDS             2629..3075
                     /gene="ivy"
                     /locus_tag="ACHKUB_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO6655458.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(3118..5562)
                     /gene="fadE"
                     /locus_tag="ACHKUB_002449"
     CDS             complement(3118..5562)
                     /gene="fadE"
                     /locus_tag="ACHKUB_002449"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO6655459.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILIPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTTEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDQLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMVVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTLSKHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            5802..6380
                     /gene="lpcA"
                     /locus_tag="ACHKUB_002450"
     CDS             5802..6380
                     /gene="lpcA"
                     /locus_tag="ACHKUB_002450"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO6655460.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            6585..7352
                     /gene="yafJ"
                     /locus_tag="ACHKUB_002451"
     CDS             6585..7352
                     /gene="yafJ"
                     /locus_tag="ACHKUB_002451"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO6655461.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFFLGERVV"
     gene            complement(7323..8063)
                     /gene="dpaA"
                     /locus_tag="ACHKUB_002452"
     CDS             complement(7323..8063)
                     /gene="dpaA"
                     /locus_tag="ACHKUB_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO6655462.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEEK"
     gene            8375..9124
                     /gene="yafL"
                     /locus_tag="ACHKUB_002453"
     CDS             8375..9124
                     /gene="yafL"
                     /locus_tag="ACHKUB_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6655463.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            complement(9399..11111)
                     /locus_tag="ACHKUB_002454"
                     /pseudo
     CDS             complement(9399..11111)
                     /locus_tag="ACHKUB_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11083..11868
                     /gene="lafU"
                     /locus_tag="ACHKUB_002455"
     CDS             11083..11868
                     /gene="lafU"
                     /locus_tag="ACHKUB_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO6655464.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            11939..12994
                     /gene="dinB"
                     /locus_tag="ACHKUB_002456"
     CDS             11939..12994
                     /gene="dinB"
                     /locus_tag="ACHKUB_002456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO6655465.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELECRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12991..13443
                     /gene="yafP"
                     /locus_tag="ACHKUB_002457"
     CDS             12991..13443
                     /gene="yafP"
                     /locus_tag="ACHKUB_002457"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6655466.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            13633..14772
                     /locus_tag="ACHKUB_002458"
     CDS             13633..14772
                     /locus_tag="ACHKUB_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6655467.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFVELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            14769..15383
                     /gene="prfH"
                     /locus_tag="ACHKUB_002459"
     CDS             14769..15383
                     /gene="prfH"
                     /locus_tag="ACHKUB_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6655468.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(15440..16897)
                     /gene="pepD"
                     /locus_tag="ACHKUB_002460"
     CDS             complement(15440..16897)
                     /gene="pepD"
                     /locus_tag="ACHKUB_002460"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6655469.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            17158..17616
                     /gene="gpt"
                     /locus_tag="ACHKUB_002461"
     CDS             17158..17616
                     /gene="gpt"
                     /locus_tag="ACHKUB_002461"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6655470.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            17708..18952
                     /gene="frsA"
                     /locus_tag="ACHKUB_002462"
     CDS             17708..18952
                     /gene="frsA"
                     /locus_tag="ACHKUB_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6655471.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            19010..19411
                     /gene="crl"
                     /locus_tag="ACHKUB_002463"
     CDS             19010..19411
                     /gene="crl"
                     /locus_tag="ACHKUB_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6655472.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(19450..20505)
                     /gene="phoE"
                     /locus_tag="ACHKUB_002464"
     CDS             complement(19450..20505)
                     /gene="phoE"
                     /locus_tag="ACHKUB_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO6655473.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINYQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            20793..21896
                     /gene="proB"
                     /locus_tag="ACHKUB_002465"
     CDS             20793..21896
                     /gene="proB"
                     /locus_tag="ACHKUB_002465"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO6655474.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            21908..23161
                     /gene="proA"
                     /locus_tag="ACHKUB_002466"
     CDS             21908..23161
                     /gene="proA"
                     /locus_tag="ACHKUB_002466"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO6655475.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNSSTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            23276..23351
                     /locus_tag="ACHKUB_002467"
     tRNA            23276..23351
                     /locus_tag="ACHKUB_002467"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23309..23311,aa:Thr,seq:cgt)
     gene            complement(23478..23816)
                     /locus_tag="ACHKUB_002468"
                     /pseudo
     CDS             complement(23478..23816)
                     /locus_tag="ACHKUB_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(23867..24823)
                     /gene="paoD"
                     /locus_tag="ACHKUB_002469"
     CDS             complement(23867..24823)
                     /gene="paoD"
                     /locus_tag="ACHKUB_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO6655476.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(24834..>26184)
                     /gene="paoC"
                     /locus_tag="ACHKUB_002470"
     CDS             complement(24834..>26184)
                     /gene="paoC"
                     /locus_tag="ACHKUB_002470"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO6655477.1"
                     /translation="ATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLY
                     AGANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDT
                     QVDPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNL
                     LEKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFP
                     VSAGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHE
                     ATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRIL
                     NPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFL
                     DDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
BASE COUNT         6267 a         6795 c         6819 g         6303 t
ORIGIN      
        1 aacgcgtttg atattaacga cttcagccca gtctgttacc agttgagcat ggtgataacg
       61 tgcaccatcc agaatgatgt tgactgtttg attataatca ggataaacct ttcttatctc
      121 attgaaaaac cgggatatgc ttaacgcatt aatggttggg tactcactga tgagtgggcg
      181 ttccggagac ttcagattta gcgcacccat gatattcagc cgtgtacggc tacctgttgt
      241 ttctattgct ttccggtgcc cttttcgtat ccagccataa ctgattttgg tggcctgagt
      301 ggggtggaca gcatctataa aaagaacagg ttcatcgcct gctttttctt tcaggtcttc
      361 ataatattca atgaattgcg cctgcctctc ttcgctgaat ttatgaggcg ttccagccgg
      421 ttttttgtaa gagaagccgt tacgatgcaa ccatttgttc ataccggcaa cgctgaacaa
      481 aattccccat gtgtggagaa caaaggcgat aatttcatga gtatggtgaa acaaattatc
      541 agataaatgt ttgattaaga gtgatgtttg ttcagcactg agtttactgt ctgaaccacc
      601 gttttcgggc tttaacttcc ctttattcat aaactcatgg atatgatgat caacggtggt
      661 ctgatgcagg cgcagagcct gagcaatcat gactgaactc cagccttcag aagccagaag
      721 aatggcttta atacgatcgc agacccgctt gttacgactg gtatcgtgaa ggcgttccag
      781 ctctgctttt tgtagatcgg agatgaagat tttcatgttg cgtagcatga tcctctgtca
      841 tgacaaaatc aagcatcttc aatgatcacg ggtatagcat cattattcta taacatgaat
      901 aaacggattg aatgtattat gttaaaaatg agtttatacg taataatatt gttattttcc
      961 tttcagatta gtgcagccat tactagcaag gaaagtgagg ctgtttcacc attattaatg
     1021 gatgctaatt cttcactaac aatggaaata tttctgaata ttccacatca tcggaaccat
     1081 cgcaacaggg cgtatttcct gtaatatgta ctcgacttca tcctagatcc gtaatgaaac
     1141 gccagttatt aacctgctag gggcctgttt ttataattgg tgatgatcct ttttctctgc
     1201 gctggatgag tgaacatctg gagatcctta aatcgctaaa tacccttggt ttggttgtta
     1261 atgttgaatc agtagaacga atggaggtat tacagaagcg ggctgatggg ttattgttat
     1321 tgccagttat ctgcgataat ttcgtgcaaa tgctgcaatt aaatgcttat ccggtattga
     1381 tcacagagat ggaaatatca caatgattaa taacataatt actgctaata atatcgtaat
     1441 tttgaaagtg gtaatcctgt tcacggcact ataattagag ttatgtcttg tatccgttca
     1501 gtataatttc acgaatgctt tattaaccgg atgcgtgaat gtcattcttc agatgacaaa
     1561 gacgtcatcg cgattgatgg aaaagcactt ccacactcct atgataaaag ccgtcgcagg
     1621 agagcgggct ttcgtagtct taccccgact ctccccggcc ctaaacgcaa cacccactca
     1681 cagcaaccta aactcatccg catcccgcca tgccggaaac ttttctctat attcccttaa
     1741 tacagccatc gacaactccg catcaatgcg cgttgcctga tgcgcgtcgg cagtagcgat
     1801 aatctcgccc tgtggattaa tcacccggct gtcaccgcga taatggcagc cgttgccatc
     1861 gctgccgacg cgattgcatc ccgctacata cgtctgattc tcaatcgcgc gggccgtcag
     1921 caatgcctgc cagtgcagag agcgcggagc aggccagttg gcgacgtaca gggcgaggtc
     1981 ataatcgttg agattgcgcg accacactgg aaaacgtaag tcgtagcaca ccagcggcaa
     2041 aatacgccag ccgcgccatt ccacaatcac tcgcgcattg cccgctttat aatgtagatg
     2101 ctcatctgcc atgcggaaca gatgacgctt atcataaaaa tgtaccgtgc cgcccggctc
     2161 aaccagcaaa aagcggttaa ccgaaccaga ctccgtttgt aatgcaacac tgcctgcaat
     2221 cagcgcattg tactgctgcg ctttggctgt catccagttc actacgtcat cttgtgctag
     2281 cgacgaagct gccgcttcca tggcaaagcc gctggtaaac atttccggta gaacgatcac
     2341 atcgcgcccg gtaatacctt ccagttgacg atcaaaatgg cgcagattgg cagggccatc
     2401 catccacacc agtggttgct gcaaaagcgt aatcttcaaa ccaggcacgg tgtacaactc
     2461 ctttatgcga agggttttat aactttaaca ccttatcagg cagttgcctt agcgcagaat
     2521 aaattgataa caaatgctga tattggaaat atctgatttg caaattatcg tgttatcgcc
     2581 aagctttagg aggttaataa catgggcagg ataagcccgg gaggaatgat gtttaaggca
     2641 ataacgacag tcgccgctct ggtcatcgcc accagtgcaa tggcgcagga tgatttaacc
     2701 attagcagcc ttgcaaaggg cgaaaccacc aaagctgctt ttaatcagat ggtgcaaggg
     2761 cataagctgc ctgcctgggt gatgaaaggc ggtacgtata ctcccgcaca aaccgtaacg
     2821 ttgggagatg agacgtatca ggtgatgagc gcgtgcaaac cgcatgactg tggctcgcaa
     2881 cgtatcgctg tgatgtggtc cgagaaatct aatcagatga cggggctgtt ctcgaccatt
     2941 gatgagaaaa cgtcgcaaga gaaactcacc tggttgaatg tgaacgatgc gctttctatt
     3001 gatggtaaaa cggtgctgtt cgcggcgttg accggcagcc tggaaaacca tccggatggc
     3061 tttaatttta aataattagc ggataaagaa acggagcctt tcggctccgt tattcaatta
     3121 cgcggcttca actttccgca ctttctccgg cagctttacc ggcttcgtcg ccagctcttc
     3181 tggatcaaag tcatcaacgt taatactacg cagacggctt tcttcagctt tcaccagaat
     3241 agcggcttca tctttatcaa tcagcccctt cgccagtgcg ttgtgcgcca gttcatccag
     3301 acgggtaaac ggcaggtttt tacccaactc tttacagatc cgctgatgaa tcgggtcggc
     3361 ggcaatcaca tccaccagcg cctcttccag taaaccaacc gggttatgct tgctcagtgt
     3421 caggtactga ccgcgaccaa tacgtgaacg ggtggcattc ggcacttgta aaatcttcgc
     3481 cactttatga tccagcttgt cagacggtgc caggtaatga cgtccggtcg ggaagatcac
     3541 cacattcagc agcccggcaa ccacgcggtt cgggaagttt tgcagtaaat cgtccatcgc
     3601 ctgttctgcc tgatacagcg catcctgtac accccagtgc accagcggca ggtcggcttc
     3661 attacggcct tcgtcgtcat aacgcttcag aacggcagag gcgaggtaaa gctgacttaa
     3721 aatatccccc agacgtgccg agatacgctc acgacgtttc aggctgccgc ccagcaccac
     3781 catcgagaca tcagaaagca gggcgaggtt ggcgctcagg cggttcaggt gctgatagta
     3841 gcgtttagtg gcatcgccgg ttggtgtgct gctggttaaa ccgcgcgtca ggcccagcca
     3901 gaagctgcga actttgttgc taccgacgtg accgatatgt ttaaacagga gttgatcgaa
     3961 cgcgttaacg tcattgttct tcgccgcttc catctcttcc agcacgtacg gatggcaacg
     4021 aatcgctcct tgtccgaaga tcatcatgct gcgggtcaga atgttggccc cttcaacggt
     4081 gatggcaatc ggtgcgccct ggtaagcacg ggccaggaag ttgctttgcc cgagcataat
     4141 gcctttacca ccagtgatat ccatcgcatc aataatcgac tgctgcccgc ggtgggtaca
     4201 gtgatactta acgatagccg acagcacggc aggtttttca ccgagcataa tgccgtaggt
     4261 aatcagcgat gccgcagcat ccattacata agcgtttccg gcaatacgcg ccagcggctc
     4321 ttcaatccct tccatcttac cgatagagat tttgaactgc cgacgaatgt gcgcatacgc
     4381 gcccgttgcc agcgctaccg atttcacgcc gccggttgag ttggaaggca gggtgatgcc
     4441 gcggcctacc gagaggcact ccaccagcat ccgccagcct tgcccggcca ttttcggccc
     4501 gccgatgatg taatcgatcg gcacgaacac atctttaccg cgcgtcggtc cgttctggaa
     4561 cggtacgttc agcgggaagt ggcgacgacc aatttccaca cccggcgtgg tggtcgggat
     4621 cagcgcacag gtaataccta aatcttctgc accaccgagt aatttctccg ggtcggagag
     4681 tttaaacgcc agaccaagca cggtcgcaat cggtgccagc gtaatgtagc gtttgttcca
     4741 ggtcagacgc atccccagca cctgctggcc ctgccattcg cccatgcaga caatcccggt
     4801 atccggaatc gcgcccgcat cggaacccgc ttcggggctg gtcagtgcaa agcaagggat
     4861 ctcctgacca cgcgccagac gcggcagata gtgatttttc tgctcgtcag tgccgtaatg
     4921 ttgcagcagc tcgcccgggc ctaatgagtt cggcacgccg acggtaatcg ccaggatccc
     4981 gctcacgccg gagagttttt gcagcacgcg agactgggca taagccgaga actccagccc
     5041 gccgtactct tttttgatga tcatcgcgaa gaaacgatgc tctttaaggt acgcccacaa
     5101 ctccggcggc aggtccgcca gctcatgagt aatctggaaa tcattcgcca tccggcaggc
     5161 ttcttctacc ggaccgtcga gaaacgcttg ctcttcagtg gtcaggcgcg gctgcggata
     5221 gttatgcagc tttttccagt ccggcttgcc ctggaacaag tcgccctccc accaggtggt
     5281 gcccgcgtcg atcgcttctt tctcggtgcg cgacatcggc ggcatcactt tgcggaatcc
     5341 acgaaacact ggcgcagaaa tcatcgattt acgcataggg gcaaagttaa acggtatgag
     5401 gataatggcc agaggcacta gtacccacgc cgaccacaga ccagcaacgc cgaggacggc
     5461 tgtccaggcg agcaaaatca gactgctgat aaataagctc acgcggtgat agaacaatgc
     5521 gccgagcagg acaaccgtag cgagaatact caaaatcatc ataacgaaaa gccccttact
     5581 tgtaggaggt ctgaccactt gtgatgatat ggttgtagtg gatgtaaaaa catttagcaa
     5641 tgtgtttaca atataattac aacaaagctc acattgttgc tgtttttatc cgcacttcag
     5701 gtcaaaaagt cctggtcata gcacctgccc gtacttctcg cttttggcgg tatccggtac
     5761 actgcatttt gtctattaca tttatgctga aggatatcct catgtaccag gatcttattc
     5821 gtaatgaact gaacgaagcg gcggaaacgc tggctaactt tttaaaagat gacgccaata
     5881 ttcacgccat tcagcgcgcg gcggtcctgt tagcagacag ctttaaagcc ggtggcaaag
     5941 tgctttcctg tggcaacggc ggctcccatt gtgacgccat gcactttgcc gaagagttga
     6001 ccggtcgcta ccgtgaaaac cgtccgggct acccggcgat tgccatttct gacgttagtc
     6061 atatttcctg cgtcggtaac gattttggtt tcaatgatat tttctcccgt tacgttgaag
     6121 cggtaggtcg cgaaggcgat gtactgctgg ggatctccac ctccggtaac tctgcaaacg
     6181 tgatcaaagc gatcgcagcg gcgcgtgaga aggggatgaa agtgatcacc ctgacgggta
     6241 aagacggcgg caaaatggct ggcacggcgg atattgaaat tcgcgttccg cactttggtt
     6301 atgccgaccg cattcaggag atccacatta aagtgatcca tatcctgatc cagttaattg
     6361 aaaaagagat ggttaagtaa gtctggcgta ggccagataa ggcgtttacg ccgcatccgg
     6421 catttgtgct ctgatgcctg atgcgacgct gacgcgtctt atcatgccta caatctgcac
     6481 ccgaaccgta ggccgaataa tgcgttcacg ccacatccga cctgaaaatt cttaaatcaa
     6541 tcttcgccgg gggccatgcg ctcccgctgt tgtggaggtt acccatgtgc gaattgctcg
     6601 ggatgagcgc caacgtccct accgatatct gctttagttt caccggactt gtacagcgtg
     6661 gtggtggaac cgggccacat aaagatggct ggggcattac cttttacgaa ggtaaaggct
     6721 gtcgcacatt taaagatccg caacccagct ttaattcccc catcgccaaa cttgtccagg
     6781 actacccgat aaaatcctgt tcggtggtgg ctcatattcg ccaggctaat cggggcgagg
     6841 tggcgctgga aaatactcac ccgtttaccc gcgagttatg ggggcgtaac tggacttatg
     6901 cccataatgg acaactgacg ggctacaaat cactggaaac cggcaacttc cgcccggtcg
     6961 gtgaaaccga tagcgaaaaa gccttttgct ggctcctgca taaattaacg cagcgttacc
     7021 cgcgcacacc gggcaacatg gcggcagtgt ttaaatatat cgcctcactg gcggatgaac
     7081 tgcggcagaa gggcgttttc aacatgctgc tttctgacgg gcgctatgta atggcgtatt
     7141 gctcgactaa tttacactgg atcacccgcc gcgcgccgtt tggcgtggca acgttgctgg
     7201 atcaggatgt ggaaatcgac ttcagctcgc agaccacacc gaatgatgtg gtcacggtga
     7261 ttgcgacaca gccgctgacg ggcaatgaaa cctggcaaaa gattatgcca ggtgaatggc
     7321 gcttattttt cctcggggag cgtgtagttt gatgccagtt gtggctgcac aacttcgtgg
     7381 cttaacggct tgctgaccac gtaacgacca ttgaccacag aaacggtcgg tggcttacgg
     7441 gtttgctcaa agtagtcgta gcccggcttc agttgctccc agaagtcctt aaagttggaa
     7501 tatttatggc gcttcatatt ggcgtcggtc atacggaacg ggtaaatact cacttgcacg
     7561 ctcggctgac caaacaccag cgcaccagta acgaactgga agatctcatc aataccctga
     7621 ttggtcatcg cgtagcagcc gatggaaaca caatcgccgt gaatcatcag gtatttccct
     7681 tcataaccat gcgcacggtc ataggcgttt gggaatccaa tattaatcgc tttgtagtaa
     7741 cggctgtctg gttttaactg attacgctgg acgctataaa acccttccgg gcttttgaaa
     7801 tcgccctgac gctgttttgg ccctaatccg ccggaatatt tacagatttt atagctgtcg
     7861 agcagttgat attgctcgcc cattttgacg tagagatcga gcgtacgttc ttccttgaag
     7921 atctggatgt agacagggga tcccatcaac tgctgcttat actctttgct cactggcgtg
     7981 gtcgagctac tgctgcccag caaaccggca aacgaaacgc acgggatcaa caacatcgca
     8041 agaattaatg cgattttacg catactgctt attccttgat aaaacggtta cacacgccag
     8101 gacggcaaaa tgaatcccaa atcggaatag tctggatttg gaaggctcac attatcacca
     8161 aaagagtttt acgcaagcct gtcggcgcag ggtttacaaa tttagcacag gggcagtttt
     8221 caagagtccg aaaagcgcat ggattgtaga tttatttctt atttcctaag attatgaaac
     8281 cgcaatagta atatgcaaat aagcaattaa taatcccgtt tactcctccg gtcttttaaa
     8341 attctcacct tcattcagct ggtttatgtc cttcatgtct tcgttcttgc acggcagatt
     8401 tctgcatcca ggcgtgtttt cgttatgcgt tttgctccct ttactcgcca gcgccaccac
     8461 atcacatatc tcttttagct acgccgcccg ccagcggatg caaaaccgtg cgcgtttatt
     8521 aaaacagtac caaacccatc tgaaaaagca ggccagctat attgtggaag gcaatgccaa
     8581 aagcagaagg gcgctgcgcc agcataatcg ggagcagata aaacagcatc cagaatggtt
     8641 tcctgcgccg ctcaaggcga gtgacagacg ctggcaggcg ttggtggaga ataatcattt
     8701 cttaagcagc gaccatctac ataacatcac cgaagtggcg attcaccgcc tggagcagca
     8761 gctaggtaag ccttatatct gggggggcac gcggcctgat aaaggcttcg actgtagcgg
     8821 gttggttttt tatgcctaca acaagatcct tgaggctaag ctcccgcgca cggccaatga
     8881 gatgtaccac taccgtcggg caacgattgt ggcgaataac gacctgcgcc ggggagattt
     8941 gctgtttttc catatccaca gtcgtgagat agccgaccat atgggcgtat atcttggaga
     9001 cggtcagttt atcgagtcac cgcgcaccgg ggaaaccatt cgggtaagtc ggttagccga
     9061 acctttctgg caggaccatt ttttaggcgc gcgacggatt ttgacggaag agacgatttt
     9121 gtaggccgga taaggcgttc acgccgcatc tggcggttgt gcgccggtgt ctgatgcgac
     9181 actacgcgtc ttatcatgcc gacaaaaccg cactcaccag taggccggat aaggcgttca
     9241 cgccgcatcc ggcggttgtg cgccgatgcc agatgcgacg ccacgcgtct tatcatgccg
     9301 acaaaaccgt gctcacccgt aggccggata aggcgttcac gccgcatccg gcggttgtgt
     9361 accggtgcca gatgcgacgc agacgcgtct tatcatgcct atatcagcgc ccccattatc
     9421 ttcaactcca gctcatccgg cacttcgtta tacgacagca catgcagccc cggcgcaaac
     9481 aaccttgcgt agcgcgccag caaggggcgc agctgcggcg gcaccagcag caccggatct
     9541 ttccccgccg ctttcatctg ctccttcacc tgaggcatcg tgctctggaa ctggttgagc
     9601 atattcggat ccactggcac gctatcaagc atcactttgc cggcctgttg cgcctgattc
     9661 accacgttgg tcagcagatt ttccagctca ttattcagcg tatacaccgt cagctcctgc
     9721 ttgcgaacga acggatgggt aatgctgcgc cgcagcgcca ggcgcacatc ggccgccagc
     9781 agaatatgat ctttagtcac cgcactactg gcgaccaaca cggtggcgat ggtgacgata
     9841 tcgcgcaggg aaacgccttc agtcagtagc gcccggtaca ctttcagcaa ctggctgtaa
     9901 ttgagcgccg cgctcaaatc ttccgccagt cgcggtgcca tcgacgacaa acggttatgc
     9961 agctgagtaa tatcgtcata gttaaacaaa tcaggaatat agctgcgcac aatcttattc
    10021 acatgggtgg caattacgct ggcgctgtcg atcacctgat accccatatt cagcgccttc
    10081 gctttctgcg cgggctgaat ccaggtgacc ggcatcccgt acgccgggtc gttccccagc
    10141 acgccgtcta tctcgccgta ggtttcgctg gagggcaggg ccatcagttt atctgccgga
    10201 atatccgctt catcagcctt aatgccgttg atgaaaatgg cgtactggct gggcttgagg
    10261 cggaagtttt cccgaatacg gatctccggc agcagcacgc cgttgccgtc ggaaatcacc
    10321 tgacgtacgc cgcgaatact ctgggtgagc gggttgccct gcgctttatc caccagcgcc
    10381 accagtttgt agccgaggct taaactgata ggttcgatca gcggaatagt ttcccaactg
    10441 acctgttgct cgctggtttc tgtcatcgta cgggttagcg tttcgaggct tttctcttcc
    10501 gcctccgccg cttgcgggcg cttgctcatc cgccagccgg taaaaccaag cagggcgctg
    10561 aacatcagga acgggaaatg cggcattccc ggcaccaccg ccagcacgaa cataattcca
    10621 gtggcggtgt agagaaccga cgggcttgcc agcaactgat tacgcacgtc atgggcgata
    10681 tcaccgttat cgctgacgcg ggtgacgata atcgccgccg cggtggagag cagcagggaa
    10741 gggatttgcg ccaccagccc atcgccgata gtcatcagca catattgctg gaaggcggcg
    10801 tcggcgctca ggttgtattt gaagatgccg atacagacgc cgccgatcag gttgattgcc
    10861 agaatcatca tcccggcgat ggcgtccccg cgcacaaatt ttgatgcccc gtccattgcg
    10921 ccgtagaaat cggcctcgct ggcgacatct ttacgccggg tttgcgcctg cgtctggttg
    10981 atcaacccgg cgttaagatc ggcgtcaatc gccatctgtt tgccgggcat cgcgtccagg
    11041 gtaaagcggg cagaaacctc ggaaatacgc tcggccccct tggtgacgac gataaagttg
    11101 attgtcaaca gcgtcagtaa atccgagcgt gaaagcatta tcgccgcgct gcacgggcag
    11161 tcgattttca atggtggcgg gctgtcgccg ttaaataaaa tcagcccgtc tcatccgcca
    11221 aaacctgcca ccgtggcggt gccggaagag acggagaaaa aagcgcgcga cgtcaacgag
    11281 aaaacggcgc tgctgaagaa gaaatccgcc accgagcttg gcgagctggc aaccagtatc
    11341 aataccatcg cccgtgatgc gcatatggaa gcgaatctgg agatggagat tgttccccag
    11401 ggattacgcg tgctgattaa agacgaccag aaccgcaata tgtttgaacg cggcagcgcg
    11461 aagattatgc cgttctttaa aacgctgctg gtggagcttg cgccggtgtt cgactctctc
    11521 gataacaaaa ttattattac cgggcatacc gatgcgatgg cctacaaaaa caatatctac
    11581 aacaactgga acctttcggg cgatcgcgcg ctttcggctc gtcgggtgct ggaagaggcc
    11641 ggaatgccgg aagataaagt gatgcaggta agcgcaatgg cggaccagat gttgctggat
    11701 gccaaaaatc cgcaaagcgc gggcaaccgg cgcattgaga ttatggtgct gaccaaaagt
    11761 gcgtccgata cgctgtatca atactttggt cagcatgggg ataaagtggt gcagccgctg
    11821 gtgcaaaagc tggataagca gcaggtgctt tcgcagcgag cgcgttaaat gctgaatctt
    11881 tacgcatttc tcaacccctg aaattactgt atactttacc agtgttgaga ggtgagcaat
    11941 gcgtaaaatc attcatgtgg atatggactg ctttttcgca gcggtggaga tgcgcgataa
    12001 tcccgccctg cgcgatatcc ctattgctat tggcggcagc cgcgaacgac ggggggtgat
    12061 cagcaccgcc aattatcccg cgcgtaaatt tggcgtacgt agcgctatgc cgacagggat
    12121 ggcgctcaaa ttatgcccgc atctcacctt gcttccaggg cgctttgacg cctacaaaga
    12181 agcctcaaat catatccgcg aaattttctc gcgctatacc tcgcgcattg aaccgttatc
    12241 actggatgaa gcctatctcg atgtcaccga tagcgtccat tgccacggtt ctgcgaccct
    12301 catcgcccag gaaatccgcc agacgatctt caacgagctg caactgacgg cgtctgcggg
    12361 cgttgcgccg gtcaagtttc tcgccaaaat tgcttccgac atgaataaac ccaacggtca
    12421 gtttgtgatt acgccggcag aagttccggc atttctgcaa accttaccgc tggcaaaaat
    12481 tcccggcgtc ggcaaagtct cggcggcaaa actggaagcg atggggctgc ggacctgcgg
    12541 tgatgtgcaa aagtgtgatc tggtgattct gcttaaacgc tttggcaaat ttggccgcat
    12601 tttgtgggag cgtagtcagg ggattgacga gcgcgacgtt aacagtgaac ggttgcgaaa
    12661 atccgtcggc gtggaacgca cgatggcgga agatattcat cactggtctg aatgtgaagc
    12721 gattattgag cggctgtatc ctgaacttga atgccgtctg gcaaaggtaa aacctgattt
    12781 actgattgct cgccaggggg tgaaattaaa gttcgacgat tttcagcaaa ctactcagga
    12841 gcacgtctgg ccgcggctga ataaagctga tctaatcgcc accgcgcgta aaacctggga
    12901 tgaacgtcgc ggcgggcgcg gtgtgcgact ggtggggctg catgtgacgt tgcttgaccc
    12961 tcaaatggaa agacaactgg tgctgggatt atgaataaca tacaaataag aaactatcag
    13021 cctggtgatt ttcagcaact atgcgctatt ttccttcgag ctgttacgat gacggcgagt
    13081 cagcactatt ctccacagca aattgccgcc tgggcgcaga ttgacgaatc tcgctggaag
    13141 gagaaactcg cgaaatcaca agtgcgggtt gcagttatta atgcacaacc ggttggtttt
    13201 attacccgca ttggacatta tatcgatatg ttatttgttg aaccggaata cacccgacgc
    13261 ggagttgcca gcgctttgtt aaaaccattg attaagtctg aatccgaact tacggtggac
    13321 gccagcataa ccgcaaaacc cttttttgaa cgttatggtt ttcagacagt taagcagcag
    13381 cgcgttgaat gccggggaac gtggtttact aatttttata tgcgatataa accgcaacat
    13441 taaatccagc ttgcaatgaa aataacgccc gtctagtatg tgcgcgtttt cttattccac
    13501 aaactgcaag gaggtaaatc atgacaaatc ctttatcaat gactctttgc agacctttcc
    13561 aggattaatt cttttttctt gccctggatt cgtctgccat ttcctgattt ttattcataa
    13621 atggaatggt acatgggcaa atatattcgt cccttatccg atgcggtatt taccatcgca
    13681 tctgatgacc tgtggatcga gagtttagcg atccaacaat tacacaccac ggcaaattta
    13741 cccaacatgc agcgcgttgt ggggatgcca gatttacatc ccggacgcgg ctacccgatt
    13801 ggcgcagcgt tcttctctgt tggtcgtttt tacccggcac tggtcggcaa tgatatcggc
    13861 tgcggcatgg cgctatggca aacagatatt ctcgctcgca aatacaacgc cgataagttt
    13921 gaaaagcgat tatcagatct ggatgacgtt gctgaagaaa gctggctgga ggaaaacctg
    13981 ccgtcagcat ttgcacagca tccgtggcgc aactcgcttg gctccatcgg tggcggtaac
    14041 cacttcgtag aactgcaaca gattgatcaa attatcgacg cggaactgtt tgcactggct
    14101 ggtctggatg cgcagcatct gcaactgctg gttcatagcg gctcgagggg attggggcag
    14161 tctattttac agcggcatat tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac
    14221 gacgcgctac ggtatattgc ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc
    14281 cagctgatcg ctttgcgcat aatgcaacaa gttaaggcca ccggaagtcc ggttctggat
    14341 gtggcgcata actttgttag cgcgtgccaa atcggcgatc aacagggctg gttgcatcgt
    14401 aaaggcgcca cgccggatga caacggtctg gtgattattc ccggttcacg cggcgattac
    14461 tcctggctgg ttaaacctgt tgcgaacgaa aaaacgctcc actcgctggc gcatggggcg
    14521 gggcgtaaat gggggcgcac cgagtgtaaa gggcgtctgg cagcgaaata cacggcgacg
    14581 caactctcgc ggactgaact tggcagtcgg gtaatttgtc gcgataaaca actcatcttt
    14641 gaagaagcgc cacaagccta taaatcggct gaaagcgtgg tgcaatgtct ggtgctggct
    14701 gggttaatta ttcctgtcgc gcgactgcgc ccggtgctga cgctcaaaaa cagtggaggg
    14761 aaaaaaggat gatcttgcta caactctcct ctgctcaggg gccggaagaa tgttgtctcg
    14821 cagtgagaaa agcactggac aggctgatta aagaagctac ccgacaggac gtcgcggtaa
    14881 cggtgctgga aacagaaacg ggtcgctact ctgacacact gcgttcggcg ctgatttctc
    14941 tggatggcga taacgcatgg gctctaagcg aaagctggtg cggcactatt cagtggattt
    15001 gtccgagtcc gtatcggcct catcatgggc gcaaaaactg gtttctgggc attgggcgtt
    15061 ttaccgctga tgagcaggaa caatcggatg caatccgtta tgagacgctg cgttcgtcgg
    15121 ggccgggcgg tcaacatgtc aataaaaccg actcggcggt acgcgccacg catctggcat
    15181 ccggtattag cgtgaaggtt cagtcagagc gcagtcagca tgctaacaaa cggctggcgc
    15241 gattactgat tgcctggaag ctggaacaac agcaacagga aaatagcgcg gcgctgaaat
    15301 cgcagcggcg aatgttccat caccagattg aacgtggcaa cccgcgacga acgtttacag
    15361 ggatggcctt tatcgaagga taatgaagga tgaaactgcc ggaaggcgat taaacgccat
    15421 ccggcagcaa atcaaataat tacttcgccg gaatttcttt cagcagttca gtcagcagtg
    15481 tccagtaatg gccaacgctt tcgatatgaa cttgctcatc cggagagtgt ggaccggtga
    15541 tggttggccc gatagacacc atgtccattt ccggatacgg ttttttgaac agaccacatt
    15601 ccaggcccgc gtggataatc tggatgttcg gcgttttgtt gaacagacgc tgataggttt
    15661 cacgtaccag atgcattacc ggagagttag cgtccggctg ccagccagga tatgcgcctt
    15721 ttgcttctgt tttcgcacct gccagtttac ccagtgaatc cagcatgctc actacgtagt
    15781 ctttaccgct gtcgatcaga gaacggatca ggcagtgaat ttctacgtta ttgtcggtca
    15841 tggtcaccac accgacgttc agggaggttt cgaccacacc ttttgccacg tcggagttgc
    15901 ggatcacacc gttcggggtg gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa
    15961 tcagggcagc tttatcgttc gcaacagagt ccagcagcaa tgccagattt ttctctttct
    16021 ctgccagttc gtttttcagg atctcctgat aggtatccac cagagatttc agggcgtcga
    16081 ctttatcagc tgcgacagca atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg
    16141 tgccgccgtt gaaatcaata agacgcaggt ccagttcttc tgcatgaccc gccaggaagc
    16201 gcaccaacag tttgttggca ttacccaggc caacgtggat ttcaccgccg gagtgacccc
    16261 ctttcagacc ttttaaggtt aacttgaagg tttcaaaacc agctggaacc gcttcacgat
    16321 ctaaatgcag gttggaggtg aagtcgatac cccccgcaca acccatgtag atttcacctt
    16381 cttcttcgga gtcggtgtta atcagaatat cagcctgcaa ccagttgctc tgtaagccga
    16441 acgcaccgtc cataccggct tcttcggtca tggtcagcag cacttccagc gggccgtgaa
    16501 ccacgttttc gtcagccaga accgccagcg cagacgccat accaatgccg ttatctgcac
    16561 ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat ataaggctgg ataggatctt
    16621 tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac catatcgagg tgggcctgta
    16681 agacgaccgg tttacgattt tccatacctg cggtagcagg tttacgaatc aggatattac
    16741 ctacctgatc gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag
    16801 cgagttgctc ttcatgatag gacgggtgag gaatagaaca gattttggca aaaatatccc
    16861 acagcggctg tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc
    16921 cgcaaaacag tattgcaggt cacagggtta gcagaaaatt ttgtcaacac aagacaggct
    16981 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
    17041 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
    17101 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
    17161 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
    17221 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta
    17281 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
    17341 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
    17401 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
    17461 gaaatgtatc caaaagcgca ctttgtcacc atctttgcaa aaccggctgg tcgtccgctg
    17521 gttgatgatt atgttgttga tatcccgcaa gatacctgga tcgaacagcc gtgggatatg
    17581 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
    17641 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
    17701 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
    17761 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
    17821 tcttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
    17881 gcgcggtatt gacccacgtg aaatcctaga cgtccaggca cgtattgtga tgagcgatgc
    17941 tgaacgtacc gacgatgatt tatacgatac tgtgattggc taccgtggtg gcaactggat
    18001 ttatgagtgg gccacccagg cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc
    18061 gcaactcagt ggtcgacact ggctgcatgc ggctacgttg tacaacattg ccgcctatcc
    18121 tcatctgaaa ggagatgacc tggccgagca agcgcaggct ttatcaaacc gcgcctatga
    18181 agaggccgcc cagcgtctgc cgggcacgat gcggcagatg gagtttaccg tacccggcgg
    18241 tgcgcccatc accggctttt tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt
    18301 aatgtgtggt ggtctggatg cgatgcagac ggactattac agcctgtatg aacgttattt
    18361 tgcgccgcgc ggcattgcga tgctgactat tgatatgccg tcggtgggct tttcttcaaa
    18421 atggaagctc acccaggact ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa
    18481 cgtaccgtgg gtggatcaca ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt
    18541 tgccgtgcgt ctggcatacc ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc
    18601 ggtagttcat accctgttga gtgattttaa gtgccagcaa caggtgccgg aaatgtatct
    18661 tgacgttctg gcgagtcgtt tggggatgca tgatgcttcc gacgaagcgt tgcgcgtgga
    18721 gttgaatcgc tattcattaa aagtgcaagg attgctggga cgtcgctgcc caacgccaat
    18781 gttatcaggc tactggaaga acgatccgtt cagcccggaa gaggactcac gcttaatcac
    18841 ctcatcatct gctgacggta aattattaga gatcccattt aacccggtgt atcggaattt
    18901 tgacaaaggt cttcaggaaa tcaccgactg gatcgaaaaa cgtttgtgtt aaaaatttgc
    18961 taaattttac caatttggta aaacagttgc atcacaacag gagattgcaa tgacgttacc
    19021 gagtggacac ccgaagagca gattgatcaa aaaatttacc gcactaggcc cgtatattcg
    19081 tgaaggtaag tgcgaagata atcgattctt tttcgattgt ctggctgtat gcgtcaacgt
    19141 gaaaccggca ccggaagtgc gtgagttctg gggctggtgg atggagctgg aagcgcagga
    19201 atcccgtttt acctacagct accagtttgg tctgttcgat aaagcaggcg actggaagag
    19261 tgttccggta aaagatactg aagtggttga acgactggag cacaccctgc gtgagtttca
    19321 cgagaagctg cgtgaactgc tgacgacgct gaatctgaag ctggaaccgg cggatgattt
    19381 tcgtgacgag ccggtgaagt taacggcgtg agtgaaatgt gccggatgca tcacatccgg
    19441 caatatctat taaaactgat aggtcatgcc aaccgcgaca atatcatcat tattaatatt
    19501 caatttgtta tcgctatcca gttggttgat tttataatca acaaacgctg acatattttt
    19561 gttgaaataa tacgtagcac cgacgtcgat ataattgacc agatcttcat caccgatgcc
    19621 ctcaatatct ttccctttcg ataagacata acccagcgat ggacgcagac caaagtcgaa
    19681 ttgatattgt gcgaccgctt caaagttctg agttttattg gcaaagccgc cagatattgg
    19741 cgtcattttg cgtgtttcag aataaaaagt tgccagataa atattattgg catcgtattt
    19801 cagacctgtt gcccatgctt ctgcacgctt gcctgtgcca cggctttgca ggttctgctc
    19861 gttggtgcga tcggagttgg tataggcccc actaatggcg aaatcgctgc cgccaaagtc
    19921 atatgtcaat gacgtgccga agccatcgcc gttttgcttt ttaacatcgc ggttttcgtt
    19981 tttcccttga tattgcaggg ttaagttcag accatcgata acgccgaaga agtcggtgtt
    20041 ccgatacgtc gccagaccgc tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga
    20101 gtcgccacca aattccggga acatatcggt ccaggcttcc acgtcataca acgcgcccag
    20161 gttacgacca taatcgaaag aacccaaatc tttatatttc aacccggcaa aagcgagacg
    20221 cgttttttgc tgtgcagtat cactctccgc tttatttccg gcaaactctg cttcccaacg
    20281 gccataacca gtcagttgat agttaatttg tgtttcacct ttaaaaccaa aacggatata
    20341 actctggtcg ccatctttac tgtcgttatc actcatataa tgcatggctt taactttgcc
    20401 atagacatcc agtttattac cgtctttgtt atatatttct gcggcctgta cggatgcaga
    20461 tgccacaatg cccatcacca ctaatgccag agtgctcttt ttcattttca ttcctgattt
    20521 taattaacgc gcgaatattc agcgggagag tcccgttgaa aacaggaaag tttttaacct
    20581 gagattgtta aagatatatt acagattaat aatattctta aaatgtggta atttattaaa
    20641 tctgtaataa aagcgtaaac aactgccgct aggcttgctg atcccgcgca acaaaacgcc
    20701 atgctttgct cgcagatggt tggcaaccga cgacagtcct gctaaaacgt tcgtttgata
    20761 tcatttttcc taaaattgaa tggcagagaa tcatgagtga cagccagacg ctggtggtaa
    20821 aactcggcac cagtgtgcta acaggcggat cgcgccgtct gaaccgtgcc catatcgttg
    20881 aacttgttcg ccagtgcgcg cagttacatg ccgccgggca tcggattgtt attgtgacgt
    20941 cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc ggaactgcca gcgactatcg
    21001 cctcgaaaca actgctggca gcggtagggc agagtcgact gattcaactg tgggaacagc
    21061 tgttttcgat ttatggcatt cacgtcgggc aaatgctgct gacccgtgct gatatggaag
    21121 accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg
    21181 ttccggtaat caatgagaac gatgctgtcg ctacggcaga gattaaagtc ggcgataacg
    21241 ataacctttc tgcgctggcg gcgattctgg cgggtgccga taaactgttg ctgctgaccg
    21301 atcaaaaagg tttgtatacc gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag
    21361 atgtttacgg cattgatgac gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg
    21421 gaactggcgg catgagtacc aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg
    21481 acaccattat tgccgcgggc agcaagccgg gcgttattgg tgatgtgatg gaaggcattt
    21541 ccgtcggtac gctgttccat gcccaggcga ctccgcttga aaaccgtaaa cgctggattt
    21601 tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg ggcaactgcc gccattcttg
    21661 aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg
    21721 gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat cgcccacggc gtcagtcgtt
    21781 acaacagcga tgcattacgc cgtattgccg ggcaccactc gcaagaaatt gatgcaatac
    21841 tgggatatga atacggcccg gttgccgttc accgtgatga catgattacc cgttaaggag
    21901 caggctgatg ctggaacaaa tgggcattgc cgcgaagcaa gcctcgtata aattagcgca
    21961 actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca
    22021 aagcgaaatc atcctcaacg ctaacgccca ggatgttgct gacgcgcgtg ccaatggcct
    22081 tggcgaagcg atgcttgacc gtctggcact gacgcccgca cggctgaaag gcattgccga
    22141 tgatgtgcgc caggtgtgta acctcgccga tccggtgggg caggtaatcg atggcagcgt
    22201 actggacagc ggcctgcgtc ttgagcgtcg tcgcgtgccg ctgggggtga ttggcgtgat
    22261 ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa
    22321 tgcggtgatc ctgcgtggtg gcaaagaaac ctgtcgcact aacgcggcaa cggtggcggt
    22381 gattcaggac gccctgaaat cctgtggctt accggcgggt gccgtgcagg cgattgataa
    22441 tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat aaatacatcg acatgctgat
    22501 cccgcgcggc ggggctggtt tgcataaact gtgccgcgaa cagtcgacaa tcccggtgat
    22561 cacaggtggt ataggcgtat gccatattta cgttgatgaa agcgcagaga tcgctgaagc
    22621 attaaaagtg atcgtcaacg cgaaaactca gcgaccgagc acatgtaata cggtagaaac
    22681 gttgctggtg aataaaaaca tcgccgatag cttcctgccc gcattaagca aacaaatggc
    22741 ggaaagcggc gtgacattac acgcagatgc agctgcgctg gcgcagttgc agacaggccc
    22801 tgcgaaggtg gtggcggtta aagccgaaga gtatgacgat gaatttctgt cattagattt
    22861 gaacgttaaa atcgtcagcg atcttgacga tgccatcgcc catattcgtg aacacggcac
    22921 acaacactcc gatgcgatcc tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga
    22981 agtggattcg tccgctgttt acgttaactc ctctacgcgt tttaccgacg gcggccagtt
    23041 tggtctgggt gcggaagtgg cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg
    23101 gctggaagca ctgaccactt acaagtggat cggcattggt gattacacca ttcgtgcgta
    23161 aataaaaccg ggtgatgcaa aagtagccat ttgattcaca aggccattga cgcatcgccc
    23221 ggttagtttt aaccttgtcc accgtgattc acgttcgtga acatgtcctt tcagggccga
    23281 tatagctcag ttggtagagc agcgcattcg taatgcgaag gtcgtaggtt cgactcctat
    23341 tatcggcacc attaaaatca aagagttacc ccatatttaa atacaccacg tttcctcctg
    23401 tgccgcattt gtgccattgt aaccttggca attcatcaaa atactgttct gacatcaggc
    23461 agtgcaggtg cagctattta aaccaattgc tgccgccatt ctttgacgta gtcaatcagg
    23521 gcgcggagct ttggtgcaat attgcgacgc tgtgggaaat acagatagaa gcccggaaat
    23581 tgtggaagaa agtcatcaag cagcgataca agcttaccgc tttcaatata tggcctgaaa
    23641 gtttcctgag tggcaattgt tattcctccg ccggcaagag ccagcctcaa catcagacgc
    23701 agatcattag tcgtaatctg cggttcaatc gcaaggtcga aagttctccc gttttcttca
    23761 aatggccagc gataaggcgc aacctccggg gactgacgcc agccgataca cttatgggta
    23821 tttcccccgg aggcgagaaa gcgctctcca cgcccggccg caaggatcag gacgacgggg
    23881 gcaggcatga atcctcctcc tgatggagac gtacagaggc gacttctgcc agcacggaga
    23941 gtgccagagt atgcgcatcc cgggctttgg ggaatatccc gacgggtgcc cggatttgcg
    24001 ttgtttcctc cctggaccat cccagctcgt ggagcttttg cagacgtaac gtgtgggttc
    24061 gatagctgcc caatgcgccg agataaaagg gttttgcttc tcgcgcggcc tgcaacactg
    24121 gcagctcccg gttgagatca tggcacagca aaatgaccgc cgtatcggta tcgatctgag
    24181 cgctggctga ggccggaaaa agatcgaaga tatggctgtc atagcctgtg gctgctgcaa
    24241 gactcgcagt tgcctgcgcc tcaagagaac gtccgtaaat catcagcctg acgcatggcc
    24301 tgaaccccac ctcaaagcca ttgagattcc agcccgtccg ggtttgcgtg ggcaggcaca
    24361 ccagcgattg tgcttgcgga tcgtagcgca gccccgccgg ttttctctgt tccagacggt
    24421 tcagcacggc gagcagaggc tgtgccgagc gtagtttatg gagcgtcagc gtgatcccac
    24481 cgccgcaggg cagaacgatg tcaaaccacg gcgaaccttc gccatagcga atttcgcgat
    24541 cgcggcctga gcccatcatc tccagcgctt caaaagcggc agcggcctcc acgcagccgc
    24601 cagagacaaa accgcagtaa cgaccatctt cgcgaaccac catctgcgcc ccgagcgggc
    24661 gcgctgcgcc gccgcgtatc tccaccagcg tcaccagcac cgctccttta ccggacatta
    24721 gcgcctctac ggcgaagcgc agaatggtcc ggtggtcatc ggtgagaaac gcctgctctg
    24781 gcttatgcca gtgctcgtct ttgtcaaaaa gcgggtatga cattgttttc tccttaaacc
    24841 acatccggca gcttatcgag cagcttatcc agagtgatgg gataatcccg tacccgaata
    24901 ccggtggcgt tatacaccgc gttggcgata gccgcgctca cgccgcacag gcccagctca
    24961 ccgacacctt tggccttcat cggggaggat atggggtcgg tatcatccag gaaaatcacc
    25021 tcctgttttg ggatatccgc atggaccggc acctcatacc ccgccatatc gtgattaacg
    25081 aagtagccca aacggtcatc caccgccagc tcctccatca gtgccgcgcc catgcccata
    25141 gtcattgcgc caatgacctg gctgcgcgca gttttcggat tcaggatgcg tcctgcagca
    25201 cacacagcga gcatacgccg gacccgaact tctcccgtcg cgctatgcac gccgacctcc
    25261 acaaaatgcc cggcaaaggt cgactgctgg tactctttgc tcagtgttcc gaattcaatg
    25321 ctctcttccg ctgtcagtct gccgcctgcg gtggcttcat gtagcgtggc gcttcgggta
    25381 ccgttggtaa tcttgccgtc ggcaaactgc gactgctcag gatcaaaccc gactgccgag
    25441 gcaatcattt cgcgaagctt catacaggcg gcgtaaacgc cggaggtgga ggtattcgcg
    25501 ccccattgtc caccagaacc cgcagaaacc gggaaactgg aatcgccgag gtgaaccgca
    25561 acctgctcca gcggtacgcc aagcatttcc gctgccgtct gggccagaat ggtgtagctg
    25621 ccggtgccaa tgtcggtcat gtccgtttct acggtaacgg tgccgttttg ttcgaggtga
    25681 acccgagcac ccgatttttc cagcagatta ttgcgaaagc ccgccgcaac accgtggccg
    25741 actagccact ccccgtcgcg cacctgtccg ggtgtggcgt tgcgctgctt ccagccaaat
    25801 ttatccgctc cggtgcgcaa gcactcgata agctgacggc gagagaagca gcgcgtcggg
    25861 tcggcggggt caacctgagt gtcattcagg atgcgaaact cgacgggatc gatgcccgct
    25921 ttttccgcca gttcgtcgat cgcgatttcg agcgccatca gaccgggggc ttcgccgggc
    25981 gcacgcatgg cgttcccttc cggcaaatca agcgtggcga gccgcaggcc ggtatgacga
    26041 ttcgccccgg cgtagagtaa ttcgctttgc tgtaccgccg tttccggcgt gccgccgggc
    26101 aggtttccag accagctttc atgtgagata gcggtgattt tcccgctctg gtcggcaccg
    26161 atacgcaagt gctgaagggt ggcg
//
LOCUS       DAZFRN010000062        26046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFRN010000062 DAZFRN010000000
VERSION     DAZFRN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.062"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(71..151)
                     /gene="shoB"
                     /locus_tag="ACHKUB_002471"
     CDS             complement(71..151)
                     /gene="shoB"
                     /locus_tag="ACHKUB_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO6655478.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(571..951)
                     /gene="acpS"
                     /locus_tag="ACHKUB_002472"
     CDS             complement(571..951)
                     /gene="acpS"
                     /locus_tag="ACHKUB_002472"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO6655479.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(951..1682)
                     /gene="pdxJ"
                     /locus_tag="ACHKUB_002473"
     CDS             complement(951..1682)
                     /gene="pdxJ"
                     /locus_tag="ACHKUB_002473"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO6655480.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1694..2422)
                     /gene="recO"
                     /locus_tag="ACHKUB_002474"
     CDS             complement(1694..2422)
                     /gene="recO"
                     /locus_tag="ACHKUB_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO6655481.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2434..3339)
                     /gene="era"
                     /locus_tag="ACHKUB_002475"
     CDS             complement(2434..3339)
                     /gene="era"
                     /locus_tag="ACHKUB_002475"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO6655482.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3336..4016)
                     /gene="rnc"
                     /locus_tag="ACHKUB_002476"
     CDS             complement(3336..4016)
                     /gene="rnc"
                     /locus_tag="ACHKUB_002476"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO6655483.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4289..5263)
                     /gene="lepB"
                     /locus_tag="ACHKUB_002477"
     CDS             complement(4289..5263)
                     /gene="lepB"
                     /locus_tag="ACHKUB_002477"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO6655484.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5279..7078)
                     /gene="lepA"
                     /locus_tag="ACHKUB_002478"
     CDS             complement(5279..7078)
                     /gene="lepA"
                     /locus_tag="ACHKUB_002478"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO6655485.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7276..7755)
                     /gene="rseC"
                     /locus_tag="ACHKUB_002479"
     CDS             complement(7276..7755)
                     /gene="rseC"
                     /locus_tag="ACHKUB_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO6655486.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7752..8708)
                     /gene="rseB"
                     /locus_tag="ACHKUB_002480"
     CDS             complement(7752..8708)
                     /gene="rseB"
                     /locus_tag="ACHKUB_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO6655487.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8708..9358)
                     /gene="rseA"
                     /locus_tag="ACHKUB_002481"
     CDS             complement(8708..9358)
                     /gene="rseA"
                     /locus_tag="ACHKUB_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO6655488.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9391..9966)
                     /gene="rpoE"
                     /locus_tag="ACHKUB_002482"
     CDS             complement(9391..9966)
                     /gene="rpoE"
                     /locus_tag="ACHKUB_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO6655489.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9963..10118)
                     /gene="rseD"
                     /locus_tag="ACHKUB_002483"
     CDS             complement(9963..10118)
                     /gene="rseD"
                     /locus_tag="ACHKUB_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO6655490.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10374..11996
                     /gene="nadB"
                     /locus_tag="ACHKUB_002484"
     CDS             10374..11996
                     /gene="nadB"
                     /locus_tag="ACHKUB_002484"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO6655491.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRINMLQKEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11981..12718)
                     /gene="trmN"
                     /locus_tag="ACHKUB_002485"
     CDS             complement(11981..12718)
                     /gene="trmN"
                     /locus_tag="ACHKUB_002485"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO6655492.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTVRFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12850..14184
                     /gene="srmB"
                     /locus_tag="ACHKUB_002486"
     CDS             12850..14184
                     /gene="srmB"
                     /locus_tag="ACHKUB_002486"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO6655493.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKVKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14217..15098)
                     /gene="yfiE"
                     /locus_tag="ACHKUB_002487"
     CDS             complement(14217..15098)
                     /gene="yfiE"
                     /locus_tag="ACHKUB_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6655494.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
                     EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVGNELASGELIELPFGEQTQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFVAG"
     gene            15201..15788
                     /gene="eamB"
                     /locus_tag="ACHKUB_002488"
     CDS             15201..15788
                     /gene="eamB"
                     /locus_tag="ACHKUB_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO6655495.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15843..16226)
                     /gene="grcA"
                     /locus_tag="ACHKUB_002489"
     CDS             complement(15843..16226)
                     /gene="grcA"
                     /locus_tag="ACHKUB_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO6655496.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16531..17220
                     /gene="ung"
                     /locus_tag="ACHKUB_002490"
     CDS             16531..17220
                     /gene="ung"
                     /locus_tag="ACHKUB_002490"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO6655497.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(17268..18305)
                     /gene="yfiF"
                     /locus_tag="ACHKUB_002491"
     CDS             complement(17268..18305)
                     /gene="yfiF"
                     /locus_tag="ACHKUB_002491"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6655498.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSYIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18512..18931
                     /gene="trxC"
                     /locus_tag="ACHKUB_002492"
     CDS             18512..18931
                     /gene="trxC"
                     /locus_tag="ACHKUB_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO6655499.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19000..19698
                     /gene="tapT"
                     /locus_tag="ACHKUB_002493"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19000..19698
                     /gene="tapT"
                     /locus_tag="ACHKUB_002493"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO6655500.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19730..22390
                     /gene="pat"
                     /locus_tag="ACHKUB_002494"
     CDS             19730..22390
                     /gene="pat"
                     /locus_tag="ACHKUB_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO6655501.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22504..23859
                     /gene="pssA"
                     /locus_tag="ACHKUB_002495"
     CDS             22504..23859
                     /gene="pssA"
                     /locus_tag="ACHKUB_002495"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO6655502.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23905..24228
                     /gene="yfiM"
                     /locus_tag="ACHKUB_002496"
     CDS             23905..24228
                     /gene="yfiM"
                     /locus_tag="ACHKUB_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO6655503.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMLSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24225..25523)
                     /gene="kgtP"
                     /locus_tag="ACHKUB_002497"
     CDS             complement(24225..25523)
                     /gene="kgtP"
                     /locus_tag="ACHKUB_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO6655504.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25849..25964)
                     /gene="rrf"
                     /locus_tag="ACHKUB_002498"
     rRNA            complement(25849..25964)
                     /gene="rrf"
                     /locus_tag="ACHKUB_002498"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6279 a         6880 c         6488 g         6399 t
ORIGIN      
        1 cacttattta tagttattaa aggcgcgtcc gattggttca ccggacgcac cttaagtacg
       61 tttccttgtg ttataagaac agaaggatca gctgtaaaac agcaatgatg attttgatga
      121 cccgtttaat caggtatcgg caatcagtca ttcgtttttc cttaaacaag gaatgcagct
      181 gttcagatta gcccttacat ctccccaaaa ctgaacgtgc gagttattga gggtgcatgc
      241 tgcactccac accagagctt tgacgacacc actcgtttca atgggggaat tctgtggcat
      301 ggtgtaaagc acagcaaaat cttcaataac gaagccaatt ttaatgtact tacgaattgg
      361 cggtcacgtt aatctttcca tcaatattgc tttcttcgta aaggctcgag tttttatgct
      421 aaagattgca agttgcttgt aaaagataag tacactgatc cataatcgct gttgttgagg
      481 gtgcatgctg cacaaaatta aagttaaaaa gtaaaacccc cgttccttac cagttcgggg
      541 gttttacttt ttaaagagaa cggtattatt ttaactttca ataattaccg tggcacaagc
      601 atagtggcgt tcatctgcca gcgttacatg catatttaca acgccgagct tttccgccag
      661 ttttaatgcc tcaccccata gccgtagccg tggtttgccg agctcatcat tgaatacttc
      721 aaattgatta aacgccagac cattgcggat cccggtgcca aacgcttttg ctgcggcttc
      781 tttcacagca aaacgcttcg ccagaaaacg caccggctgg tggtgcgttt tccagattgc
      841 ccattcgtta tcgcttaata cgcggcgtgc caggcgctca ccggatcggg cgatcactgc
      901 ttctatgcga gcgatctcta cgatatccgt gcctaaacct aatattgcca ttagccacgc
      961 gcttccagca tcagacgctt catttctgcc accgcatctt tcagtccggt catcactgca
     1021 cgaccaataa tggcatgacc gatattcagt tcatgcatct cagggatggc ggcaatagct
     1081 ttcacgttgt gataggtcag accgtgacca gcgttaactt tcagaccgag actagcggca
     1141 aaggtcgcgg ctttggcgat acgcgccagc tcttgcgcct gttcggcgtc agttttggca
     1201 tcagcatagc aaccggtgtg gatctcgata aacggtgcgc caacctctgc cgcagctttg
     1261 atctgctctt catcggcgtc gataaagaga gaaacctgga tcccggcatc tgccagacgt
     1321 ttgcaggcat cgcgcatttt gtcacgctgc cctgcgacgt ccaggccgcc ttcggttgtt
     1381 acttcctgac gcttttccgg taccagacag caaaaatgtg gcttcgtctc aacggcgatc
     1441 gccagcatct cttcggtcac cgccatctcc agattcatgc gggtatccag cgtctgacgc
     1501 aggatgcgca catcacggtc agtaatgtgg cggcgatctt cacgtaaatg cacggtaatg
     1561 ccgtccgctc ccgcctgctc agcaataaac gccgcctgca ccggatccgg gtaagcagta
     1621 ccgcgtgcgt tacgcagcgt tgcaatatgg tcaatgttga cgcctaacag taattcagcc
     1681 atgacaatcc tcatcattca taatgtgttt tcaccgttcg cttaggcata aactgccgga
     1741 acagttccct gctctttaat ggtttaccgc caagatacgg cttaagcgcc atgcgggtaa
     1801 agcgtttcgc ggcgcgtagt gtgtctgcgt caggaaattc ccgcgcgttt aacgctttta
     1861 actgccttcc ggtgaacgtt ttattgtcga taacgacgct tgcgataaac cctttttctt
     1921 cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc gctacccgca caatgggtaa
     1981 aattgacgcc atagcccaga tgcccgagca gtgccagttc aaagcggcgc agcgcgggtt
     2041 ctggcgtacc agtggcacct gcaagagact gaatgcagtg caagtaatcg aaaaagagtt
     2101 cagagaagcg cgtctcgtat tccagtacgc gggagagaag ttcgttgatg tacagaccgc
     2161 tgtaaagcgt gataccgctt aatggcagcg ccagcgagac agcttcagca ctgcgcagcg
     2221 ttttgacttc gccacgcccg ccgaaacgta gcaagagagg ggtgaaaggc tgtaatgcac
     2281 ctttcagagt agagcgttta gagcgtgcgc ctttggcaac cagacgcacg cgacccgatt
     2341 cctccgtgaa gacgtccagc atcaggctgg tttcgctcca cgggcgacta tgcaggacaa
     2401 atgcgcgctg ccagccttcc atcggagtga ctcttaaaga tcgtcaacgt aaccgagact
     2461 gcgcagtgcg cgttcgtcgt cggcccaacc ggatttcact tttacccaca gttcaaggtg
     2521 gacaggcgct tcgaacattt cctgcatgtc tttacgcgct tcaatcccga tggttttgat
     2581 cttggcccct ttgttgccaa taaccatctt cttctgccct tcacgctcaa cgagaatcaa
     2641 accattgatg tcataaccac cgcgttcgtt agagacgaaa cgttcgatct ccacggtcac
     2701 ggagtacggc agttcggcgc cgaggaaacg catcagtttt tcgcggatga tttcagacgc
     2761 cataaaacgc tgtgagcgat cggtgatgta atcttccggg aagtgatggg tcgcttcagg
     2821 gagatgctta cgcacgattg ctgcgatagt gtcgacattc agcccggttt cagcagagat
     2881 cggcacgata tcgaggaagt tcatctgact tgccaggaac tgtaagtgcg gcagcagatc
     2941 ggctttttcc tgcacgttat ccactttgtt caccgcgagg atcaccggcg ctttgccatc
     3001 gcgcagtttg ttgagcacca tttcgtcgtc cggcgtccag cgggtgcctt caacgacaaa
     3061 aatcaccagc tcaacgtcgc caatagagct gctcgccgct ttgttcatca ggcggttaat
     3121 agcgcgtttt tcttccatat gcaggccagg ggtatcaacg tagatcgcct gatacgcgcc
     3181 ttcagtatgg atccccacaa tgcggtgacg agttgtttgc gctttgcggg aggtgatgga
     3241 gatcttctgc cccagcagtt tgttcaacaa tgtggatttg ccaacgttcg gacgtccgac
     3301 gatggcaata aatccgcagt aacttttatc gatgctcatt ccagctccag ttttttcaac
     3361 gcctgttcgg cggcagcctg ctcagcctta cgacggcttg aacctgtgcc aaccaccggt
     3421 tcactcaggc cgctgacctg gcagtggata gtaaattcct gatcgtgcgc ttcgccacgt
     3481 acctggacta ccagataagt cggcagcggc agatggcgac cctgcaaata ttcttgcaag
     3541 cgcgttttcg gatctttttg tttatcgcct gggctaattt cgtccaaacg agtttgatac
     3601 cagttgagga ttaatttctc gacggtttga atatcactgt cgaggaatac gccaccaatt
     3661 aatgcttcga cggtgtcggc gagaattgac tcacgacgaa atccaccgct tttaagttca
     3721 cctggcccta aacgtaagca ctcgcctaac tcaaattcgc gcgccagttc cgccagcgta
     3781 ttgccacgga ccagcgtggc gcgcatccgg ctcatatcgc cttcatccac acgagggaaa
     3841 cggtgataaa gcgcattggc gataacgtag ctcagaatag agtcgcctaa aaattctaaa
     3901 cgctcgttat gtttgctgct ggcactacga tgagttaatg cctgctgcaa cagttcctga
     3961 tgattaaaag tgtagcccag cttccgttga agccgattaa ttacgatggg gttcatgcga
     4021 taccaataaa taaatgcgtc aacaattcag cacacgaaac agacctgata tacatggctc
     4081 tgctaactgc ttcgctgcag tttcagttta ccggtatatg gggaccaacg ctgtttcgtg
     4141 tgccgtggca acctggaggt gccaacctta aacttcgggg gaatattcta tacacaacga
     4201 cgggggatgt cgttagccac gggagattta tctcataaat aattcacgtt gtcgccataa
     4261 cggcgacaac gtgaacgaag atgggctatt aatggatgcc gccaatgcga cttaagcgca
     4321 cacctgtcgg ccattcgcct tcttgcttat cgaaactcat ccagatagcc gttgcccgac
     4381 cgaccagatt cgcttcaggc acaaaccccc aataacggct gtccgcgctg ttgtcgcggt
     4441 tgtcgcccat catgaagtat tgtcccggag gaacaatcca ggttgccagt tgttgccctg
     4501 gctgctggta atacatcccc acctgatcct gcgcaatcgg cactgtcaga attcggtgcg
     4561 tcacatcacc cagtgtctct ttacgctcgg aaagacgaat tccattttct ttggtttcgt
     4621 ttttcggcac ttcaaagaat ccgctggtcg cttccccacc attacggcgt gagaaggtct
     4681 gaacgaaatc gctcggttcc acgtttgagt aggtgaccgg cagcgcgttt tcacacgcct
     4741 ggccggaact gcatcccggt tgaatcgtca gctctttcga gaccggatca taagtaactt
     4801 tatcgcccgg taaacccacc gcgcgcttga tgtaatcaag ctttggatct tccggatatt
     4861 taaagaccac gatatctccg cgtttcggat gaccggtttc gatcagcgtt ttctggtaga
     4921 taggatcttt aatgccataa gcaaactttt ctaccagaat aaaatcaccg attaacagag
     4981 tcggcatcat cgaacctgac gggatctgga acggttcata aataaacgaa cgcacaatca
     5041 atacgatagc cagtaccgga aaaacagaag caccggtttc cagccagcca ggcttcggcg
     5101 caaccttttt caacgttgct ttatccagtg agtcaccggc agccgcctgc gccgctgcct
     5161 gacgttcccg ccgtttaggt gcgaaaaaga atttatccac gcaccataaa atgcccgtca
     5221 ccagtgtggc aatcaccaga atcagggcaa acatattcgc catgccaact cctaagggtt
     5281 atttgttgtc tttgccgacg tgcagaatgg cgaggaacgc ttcctgcggc agctcgacgt
     5341 taccgatctg cttcatgcgt ttcttacctt ctttctgctt ctgcagcagc tttttcttac
     5401 ggctgatatc gccgccataa catttagcca gtacgttttt acgcagctgt ttcacggtgg
     5461 atcgcgcaat aatgtgcgta ccaatcgctg cctgaatggc gatatcaaac tgctggcgtg
     5521 ggatcagatc tttcatcttc tccaccaact cgcgaccgcg gttttgcgaa ttatcacggt
     5581 gggtgatcag cgccaacgca tcaacacgtt caccgttgat taatacgtct acacgtacca
     5641 tgtcggacgc ctggaagcgt ttgaagttgt aatccagaga cgcataacca cgcgaggtag
     5701 atttcaggcg atcgaagaag tcgagcacca cttccgccat cgggatctcg tacgtcagcg
     5761 ccacctgatt accgtggtaa accatattgg tctgcacgcc gcgtttttct acgcacagcg
     5821 taataacgtt gccgagatat gcctgcggca gcagcatgtg acattcggca atcggctcgc
     5881 gcagttcgta gatgttattt accgctggca gcttggatgg actgtcgacg taaataactt
     5941 ctcttgacgt ggtttcaact tcatacacta cggtcggcgc agtggtgatc agatccagat
     6001 cgtattcacg ttccagacgt tcctggatga tctccatgtg cagcaggccg aggaagccgc
     6061 agcggaaacc aaagcccagc gcgctggagc tttccggctc atagaacagt gaagcatcgt
     6121 tcaggctgag tttacccagc gcgtcacgga aggcttcata gtcgtcggaa cttaccggga
     6181 acagaccggc gtatacctgc ggtttgactt tcttaaagcc aggcagcgcc ttttctgccg
     6241 gattacgcgc cagcgttaag gtatcgccga ctggagcgcc gtggatatct ttaatcgcac
     6301 ataccagcca gcctacttcg ccacatttca gttcagtgcg gtcaacctgt ttcggcgtga
     6361 agatgcccag acggtcggcg ttataggtct gcccggtact catgactttc actttgtcgc
     6421 ccttacgcag ggtgccgttt ttaatacgga taagtgaaac aacgcccagg tagttgtcga
     6481 accatgagtc gataattagt gcctgcaacg ggccttccgg atcgccttcc ggcggcggaa
     6541 tgtcgcgcac cagacgttcg agaacgtcct gcacgccaac gccggttttc gctgaacagc
     6601 gcaccgcgtc agtggcgtcg atgccgacga tatcttcaat ttcttccgcc acgcgttcag
     6661 gatcggctgc cggcaggtca atcttgttca gtactggcac cacttcgaga tccatttcca
     6721 tggcggtgta gcagtttgcc agggtttgcg cttccacgcc ctgcccggcg tcgaccacca
     6781 gcaatgcacc ttcacaggca gccagcgaac gggaaacttc ataggagaag tctacgtggc
     6841 ccggggtgtc gataaagtta agctgatagg tttcgccgtc agacgctttg tagtccagcg
     6901 tcacgctttg cgctttgatg gtaatgccac gctcacgctc aagatccatg gaatcgagaa
     6961 cctgcgcctc catttcacgg tcagacaggc caccgcagat ctggataata cggtcagaca
     7021 gcgtcgattt accgtggtca atgtgagcta tgatcgaaaa gttacgtata ttcttcataa
     7081 agtatgatta ttgtgcctta atgcccggtt aaccaggctt ttagaagtcg ctgttctgag
     7141 cttaacgtct gtattaatag aaacgccgca ttctacacta caacattgag gcgaggaaat
     7201 gttcataccg tatggattgt ggtatctgga aacgtcctcg catttgttat gcaaaatgca
     7261 acaaagccag tgaaatcact ggctcgcgtc ttccgaagat gtttcaaatc gcacaaggcc
     7321 aggcggcaat gccacgctta agatgatcgg ttgccattct gcccgggcag caaacttacg
     7381 cgagtagccg cgcgcaatca ggaacccgcc aataccaccg aggatcgcac cacataatgc
     7441 tgcgacgtca gaagcaaaga gtagctgaaa tagcgaagcg ataaggaata atcccaccag
     7501 cggcgacata taaaccagta atgcggagct aagcaggctg ccttcggcga tccccaattc
     7561 cactttttgc cccggcacta acggttcatc acagggtacg acaatggtat gcgtggtttg
     7621 cgggccaagt ttattcaaca cgcggctacc gcaaccggcg cgtgaagcgc agctgctgca
     7681 tgaagcttta acatcacaac tgaccagcgc ctgcccgttt tgccaggaga cgacggtagc
     7741 ccactctttg atcattgcgc tgccccgaac ttaatattct cggcaatgcg tttcgccgtt
     7801 tgcggcggca gttcaccgac aatggtgatt tcggcgttat cacgtacgct tgtactgacg
     7861 gttctgcgtc cggtgcgcaa catctgatcg gtgctcgatg gcgtagcgcg gttaacgttt
     7921 accgagaagc tgaataatcc gtcggaatag agacgtgatt cgataggcat gttgtccatc
     7981 gtcggtagcg gacgtcgact actggaaact tcgctaaaac cctgtggcaa ccaggttggc
     8041 gtccagctga atttagcttt ttcacctaca ggaacagaaa gcaacggcgg caaatttgcc
     8101 ttcgccagcg tctgcatact gctactgata tcctgattga cgttaaaagc aatcacgcga
     8161 aattgttcca gcgtttcacc atcgcgatca aggagatcaa ccctcatcgg taatttcgat
     8221 tcgatgtcca tccacacgat gtagctgtag cgtgtaccat ctcgggcaac cacgcgaatg
     8281 acttcgcaaa gacgatcagc aatacgcgtg cgcccgacgg agataaagtc gtagtaagga
     8341 gaaaggcgtt tgaaatcggt atagatgagc gatggcagag aatcaacaat gtaatcgcca
     8401 ttaagcgtga acggttcaag tcccggttca aaatagctga tttcattgcc gcgctgtacc
     8461 acttcccggc gcgggccatc catttgcaac aattgtgcaa gaggacggtt atcgaggcgt
     8521 gcatgtcgat aacgcagaga ctcaacaccc tgtttattga tgctgatgaa tgacagctcg
     8581 taattcagtg actgactggc caggttcatc tgctgtaata acgccccgga cgcgggagtg
     8641 gccgaggcgt tagcagagaa taacaggcta cctgtcacta atgacatggc aaaccaaagt
     8701 tgcttcatta ctgcgattgc gttcctaaag tttgaattcc tggcacctgt acagcggctt
     8761 gctgggtttg cgcctgctca aactgaagct gttcagagtg gagtcggcgt tgcagttcgt
     8821 aatcctgcaa cattgcatta atgcgacgac gctgctcctg tacctgctgc tgttgaccat
     8881 tgtttgcggt cgcttcagaa ggtactccca ggcttaccgg gctggcttta cccatcatcg
     8941 gcagtgtatt aaataccggc gtttcgggct gctgggacgt ttcagattgt ccattatagt
     9001 gctggacgcc aacgataact gcaagcgata cgcatgctgc tacgcccatt tgggtaagct
     9061 gtgccgccca cggacgtact ttctgccaga atggcatttt ctgccattga tgcggcgcag
     9121 gctgggcttc cgggatcaat gtcgccggtt gacgtactgg ctcttcttca atggcggcca
     9181 tcacgcgtga agagatatcg aaatggagca cctcgggagt atcaccccgc attgagtcac
     9241 ggattaagtg atagctttcc caggttttct gcatttctgg gttatgagcc agttcgttaa
     9301 gcagctcact atccagcgtt tcgccatcca ttaaagcgga aagttgttct ttctgcatgc
     9361 ctaataccct tatccagtat cccgctatcg tcaacgcctg ataagcggtt gaactttgtt
     9421 atcaatagct tccctcgctc ggaagatacg tgaacgcacc gtacctaccg gacaatccat
     9481 gatagcggct atctcttcat agctcaggcc atccagctcc cgcaaggtta ttgccatgcg
     9541 caaatcttcc gggagggact caatagttcg gaaaactatc tgtctcagtt cttctgacaa
     9601 cattaagttc tcagggttcg aaatttcttt caacgcgccg ccactttcga agttttcagc
     9661 ttcaatggca tccacatcac tggaaggtgg acgacgcccc tgagcaacca ggtaattttt
     9721 cgctgtattt acagcaatcc gatacagcca tgtataaaaa gcgctatctc cccggaacga
     9781 atccagcgca cgataggctt taataaaagc ttcttgtacc acatcgggaa catcacccga
     9841 cggcacatag cgggaaacca gactcgccac tttatgctga tagcgcacta ccagtaggtt
     9901 aaaggctttc tgatctccct tctggacccg ttcaaccagg acctggtccg ttaactgctc
     9961 gctcatccga ggtaaagtct ccccaaacca aatttccacg cgctatcgaa acgccactcc
    10021 attagctgca atttgagcaa gcaaagggtt agagtgtctc gtttttgtaa agttccgtaa
    10081 cgcatctgtt tttgtttgtc atgctgtaga cggatcatta tctatcatta taagtctaca
    10141 gaatctgaac atcgcattat ctgtgtagaa atgcccattt aactgcctga agagtaaccc
    10201 aacggccttt ttatttcacc acctaatcct ccaccagcca gtaacttctc tttttctcgc
    10261 cgccctgcgt cagcgtgttt agccactgta acaaatatta aaatagcagg tgtttattcg
    10321 cacaacatga tgctatgctg accaaacggt gtttagtaaa ttaaacaaag aaaatgaata
    10381 ctctcccaga acattcatgt gacgtgttga ttatcggtag cggcgcagcc ggactttcac
    10441 tggcgctacg cctggctgac cagcatcagg tcatcgttct aagtaaaggc ccggtaacgg
    10501 aaggttcaac attttatgcc cagggcggta ttgccgccgt gtttgatgaa actgacagca
    10561 ttgactcgca tgtggaagac acattgattg ccggggctgg tatttgcgat cgccatgcag
    10621 ttgaatttgt cgccagcaat gcacgatcct gtgtgcaatg gttaattgac caaggggttt
    10681 tgtttgatac ccacgttcaa ccgaatggcg aagaaagcta ccatctgacc cgtgaaggtg
    10741 gacatagtca ccgtcgtatt cttcatgccg ccgacgccac cggtagagaa gtacaatcca
    10801 cgctggtgag caaggcgcag aaccatccga atattcgtgt gctggagcgc agcaacgcag
    10861 ttgatctgat tgtttctgac aaaattggcc tcccgggcac gcgacgggtt gttggcgcgt
    10921 gggtatggaa ccgaaataaa gaaacggtgg aaacctgcca cgcaaaagca gtggtactgg
    10981 caaccggcgg tgcgtcgaag gtttatcagt acaccaccaa tccggatatt tcttctggcg
    11041 atggcattgc tatggcgtgg cgcgcaggct gtcgggttgc caatctcgaa tttaatcagt
    11101 tccaccctac cgcactgtat cacccacagg cacgcaattt cctgttaacg gaagcactgc
    11161 gcggcgaagg cgcttatctc aagcgcccgg atggcacgcg ttttatgccc gattttgatg
    11221 agcgcggcga actggccccg cgcgatattg tcgcccgcgc cattgaccat gaaatgaaac
    11281 gcctcggcgc agattgtatg ttcctcgaca tcagccataa gcccgccgat tttattcgcc
    11341 agcatttccc gatgatttat gaaaaattgc tcgggctggg gattgatctc acacaagaac
    11401 cggtaccgat tgtgcctgct gcacattata cctgcggtgg tgtaatggtt gatgatcatg
    11461 ggcgtacgga cgtcgagggc ttgtatgcca ttggcgaggt gagttatacc ggcttacacg
    11521 gcgctaaccg catggcctcg aattcattgc tggagtgtct ggtctatggc tggtcggcgg
    11581 cagaagatat caccagacgt atgccttatg cccacggcgt cagtacgtta ccaccgtggg
    11641 atgaaagccg cgttgagaac cctgacgaac gggtagtaat tcagcataac tggcacgagc
    11701 tacgcctgtt tatgtgggat tacgttggca ttgtgcgcac aacaaaacgt cttgagcgcg
    11761 ccctgcggcg tatcaatatg ttgcaaaagg aaatagacga atattacgcc cacttccgcg
    11821 tgtcgaataa tttgctggag ctgcgtaatc tggtacaggt tgccgagttg attgttcgct
    11881 gtgcgatgat gcgcaaggag agtcgggggt tgcatttcac gctggattat ccagaactgc
    11941 tcacccattc cggtccgtcg atcctttccc ccggcaatca ttacataaac agataaaaag
    12001 cctgggttag cgccgtatac gcttcggaat agttctggtc tggcccacga atgactaagc
    12061 gatcgctaaa gcattctccc gcctgcgggg agaatgccag cagcacccga tgcggcagtc
    12121 gcgcttcgtt ttccgccaca tccgttcgca gacgtaaatg ccagcccatg cttaatgcca
    12181 gttcagtaaa accattacca atctgctctg gcagcaccac gcagaaaaat ccctcttcgg
    12241 taatgcactc cgccgcgcaa gtaagtaaag tctggtgatc aagtgaggtg gtgtagcgcg
    12301 cctgttcccg ctgcggcgtc gcgcactcca ctccttgctc ataatacggc gggttgctga
    12361 tgattaaatc aaagcgcacg gtctgctgtg ttacccactg ctgaatatcc gccgtatgga
    12421 cgttaattct ctctaaccac ggggactggg cgacattttc ctgcgcctga gtggcagctt
    12481 cactttccag ctcaacggca tcaattatca cgctgtcact ggttcgctgc gccagcatca
    12541 gtgccagcaa gccactaccc gcaccgatat caaggcaacg ttttacccca gccaccggtg
    12601 cccatgcgcc cagcaaaatg ccatccgttc ccactttcat cgcacagcga tcgtgagcaa
    12661 caaaaaactg tttaaaagta aatccattac gacgaagcac ggatgtagac tgtgacatga
    12721 aaataaaacc ttgcaggaaa aacggcgata gcaccgggtg agaacaatac ccgagaagcg
    12781 atatccatac aaacagatga agattgcagc cgtaacgtct ataatcagcg ccccacacag
    12841 aggtagaaca tgactgtaac gactttttcc gaacttgaac tcgacgaaag cctgctggaa
    12901 gccctccagg ataaaggttt cactcgcccg accgccattc aggctgccgc cattccgcct
    12961 gcgctcgatg gccgtgatgt actcggttct gcgccgacag gcaccggtaa aacggcggcg
    13021 tatctgctgc cagcgttgca gcacctgctc gatttcccgc gtaagaaatc cggtccgccg
    13081 cgtattttga tcctcacccc aactcgcgag ctggcgatgc aggtggccga tcatgcccgc
    13141 gaactggcga aacatacgca tctggatatc gccaccatca ccggcggcgt agcctatatg
    13201 aaccacgcgg aagtgttcag cgaaaatcag gacatcgtgg tcgccacgac cggacgtctg
    13261 ctgcaataca taaaagaaga gaacttcgat tgccgcgcgg ttgaaacgct gatcctcgac
    13321 gaagcagacc gtatgctgga tatgggcttt gctcaggata tcgaacatat tgctggcgaa
    13381 actcgctggc gtaaacagac cctgctcttt tcggcaacgc tggaaggcga tgcgattcag
    13441 gactttgccg agcgtctgct ggaggatccg gtggaagttt ctgcgaatcc gtccacccgt
    13501 gagcgcaaaa aaattcatca gtggtattac cgcgccgatg atcttgagca taaaaccgcg
    13561 ttgctggtgc atctgttaaa acagcctgaa gcgacccgct caattgtgtt tgtgcgtaag
    13621 cgtgaacgtg tgcatgagct ggcaaactgg ctgcgcgaag cgggcatcaa caactgctat
    13681 ctcgaaggtg agatggtaca aggcaagcgt aacgaagcga tcaagcgttt gaccgaaggt
    13741 cgcgtaaacg tactggttgc aaccgatgtt gccgcgcgcg gtatcgacat tcctgacgtc
    13801 agccacgtct ttaacttcga tatgccgcgc agtggcgata cttatttgca ccgtatcgga
    13861 cgtaccgcgc gcgccggtcg taaaggcacc gcgatttcgc tggtggaagc ccatgaccat
    13921 ttgctgctgg gcaaagttgg ccgttatatt gaagagccaa ttaaagctcg cgttattgat
    13981 gagttacgcc cgaaaacgcg tgcgccaagc gagaagcaga ccggcaagcc atcgaagaaa
    14041 gtactggcaa aacgcgccga gaagaaaaaa gttaaagaga aagagaagcc gcgagtgaaa
    14101 aaacgccatc gcgataccaa aaatattggt aagcgccgta aaccaagcgg aacgggcgtg
    14161 ccaccgcaaa cgacagaaga gtaatctcaa tgccgggttt aagcccggca ttatttttat
    14221 ccagccacaa aacactcttc aacacactga ataaaagtgt gcatcgccgg actaaccgct
    14281 tttccggcat ggtgagcgca cattgccgta atggtctgcg tctgttcgcc aaaaggcaat
    14341 tcgattaatt cgccactcgc aagttcattt cccactgcaa agcgcggcag gtaactgacc
    14401 ccgatatttg ccgcgacaca acgcttgatg ctttcgatac ttataagctc aatggtgttt
    14461 tccaccgtga tccgccgctg gcgcagcgtg ctctcaaata tctgccggaa gacacattgt
    14521 ggttcgttga taataaagct acaggcgtta tgtcttcccg gctcagtaaa atcgatatct
    14581 gcaatttgcg gtgaagccac cagcaccagc ggttgttcac ccaactctcg tcggttcaga
    14641 acatcatcat tccctacgcg ataaaaaacc ccgacatcgg cctcatcatt cagtagcgca
    14701 tcacggatca cgtaacagtt cagcgactgc aacgataaac gcacttttgg agcgcgttgc
    14761 cgaaaacgct gcaacacctg cggcattcgg taagagagca acgtttcgcc tgaaacaacg
    14821 cgtagttccc cgtccggatc cgactctttt ttcgcagcct cacgaagcgt atccatcact
    14881 cgggtaagtt catagatgtg cggcagcaac ttttttcctt cgcgggtaag gcacattcgt
    14941 cgaccaattt tctcaaacaa ttgtaccgag aactcctgct caagctgctg aatatgaaaa
    15001 gtcaccgtcg attgtgtaca gcacaatttt tgcgaagctc gcaaaaagga accctcttcc
    15061 accacggttt taagtgtaat aaaacggcgc agatccataa ccccaaacct atcgaaaata
    15121 tcgaatttag aatgtaaaaa cattcatttt tttaaatgtt ccgtgtcggg tactgtctac
    15181 caaaacagag gagataacaa gtgacaccga cccttttaag tgctttttgg acttacaccc
    15241 tgattaccgc catgacgcca ggaccgaaca atattctcgc ccttagctct gctacgtcgc
    15301 atggatttcg tcaaagtacc cgcgtgctgg cagggatgag tctgggattt ttgattgtga
    15361 tgttactgtg tgcgggcatt tcattttcac tggcagtgat tgacccggca gcggtacacc
    15421 ttttgagttg ggcgggggcg gcatatattg tctggctggc gtggaaaatc gccaccagcc
    15481 caacaaagga agacggactt caggcaaaac caatcagctt ttgggccagc tttgctttgc
    15541 agtttgtgaa cgtcaaaatc attttgtacg gtgttacggc actgtcgacg tttgttctgc
    15601 cgcaaacaca ggcgctaagc tgggtagttg gcgtcagcgt tttgctggcg atgattggga
    15661 cgtttggtaa tgtgtgctgg gcgctggcgg ggcatctgtt tcagcgattg tttcgccagt
    15721 atggtcgcca gttaaatatc gtgctcgcgc tgctgctgat ctattgcgcg gtacgcattt
    15781 tctattaatt aaaaaagcgg aagaggtcgc cctcttccgc ttagtaactt gctacttaag
    15841 ccttacaggc tttcagtaaa ggtacgagcg ataacgtcgc gctgctgttc cggagtcaga
    15901 gagttaaagc gaactgcata acctgataca cggatggtca gctgcggata tttttccgga
    15961 tgcttaactg catcttccag agtttcacga cgcagaacgt taacgttcag gtgttgacca
    16021 ccttcaacgc gaacttctgg tttcacttct actggaactt cacggtattc aatgtcaccc
    16081 agtttgctta ctgcaaccac ttcatcttct gcataacctg cttttgcaac gatgcaacgc
    16141 gcttcgcctt tttcgctgtc cagcagccag aaagagttca gcagatcgtc gttagcggct
    16201 ttagtaatct ggatacctgt aatcatgtga tgcctccccg gcaaaattat ttgatttatt
    16261 cagcctgtcg cggccaattg gtaaaaccat tgttgcttga gtgtatatat actcctcaac
    16321 aacccttgat tccttgattt aaatcaataa aaaccacaca tcgattgtgg tcgcaaatgg
    16381 attttattgt tttacatcaa cttatgcggg tgtgaaattt taccaattta catttttttg
    16441 cactcgttta agtctaaaaa atgagcatga ttttgttctg tagaaataag cagttaagct
    16501 aggcggattg aagattcgca ggagagcgag atggctaacg aattaacctg gcatgacgtg
    16561 ctggctgaag agaagcagca accctatttt cttaataccc ttcagaccgt cgccagcgag
    16621 cggcagtccg gcgtcactat ctatccacca caaaaagatg tctttaacgc gttccgcttt
    16681 accgagttgg gtgacgttaa agtggtgatt ctcggccagg atccttatca cggaccggga
    16741 caggcgcatg ggctggcatt ttccgttcgc cccggaatcg ccactcctcc gtcactgctg
    16801 aatatgtata aagagctgga aaatactatt ccgggcttca cccgccctaa tcatggctat
    16861 ctcgaaagct gggcgcgtca gggcgttctg ctgctcaata ctgtgttgac ggttcgcgca
    16921 ggtcaggcgc attcccacgc cagcctcggt tgggagacgt ttactgataa agtcatcagc
    16981 ctgattaacc agcaccgcga aggcgtggtg tttttgttgt ggggatcaca tgcgcaaaag
    17041 aaaggggcga ttatagataa gcaacgccat catgtactga aagcaccgca tccgtcgccg
    17101 ctttcggcgc atcgtggatt ctttggctgc aaccattttg tgctggcaaa tcagtggctg
    17161 gaacaacgtg gcgagacgcc gattgactgg atgccagtat taccggcaga gagtgagtaa
    17221 atttgcgggg aaatgccgga tggcagagtt gccacccggc tgatttatca ggctttattc
    17281 tgacgccacc attcaccaag caaaacgccg gttgcgacag agatattcag cccggcaacg
    17341 ttgcccgtac cgtcaatctt cacgcgcaga tcgttcggat cgcgtgcggc atccggtaac
    17401 ccttcatatt cctgacccag caccagtacc attttcgctg gcagactggt tttgaacagc
    17461 ggtttaccct gctcgctgga agtggtcact acggtgtaac ctgcctgacg gaaatcatcc
    17521 agcacgttaa caatgttgtc gccagtaatc ggctgaacgt gctctgcgcc gccttctgcg
    17581 gtacggatag ccgcccccga ctccagcagt gccgcatcct gcaccacaac gcctttcacg
    17641 ccgaagtgcg cgcagctgcg catcatgcca cccaggttat gcgggttaga ttcgttttcc
    17701 agcgccagta cacaatcctg cgcgcctgcc tggcttaccc actgctgcac ggttgtaccg
    17761 ttacgctttt tgatcaagaa gcaaacgccg ccgtgatgtt ccgtgcctga cgcttttgtc
    17821 aattccgctt catccaccac atggtacgct ttgcggtttg ccgccatcca gcgcaaggct
    17881 tctttaaaac gtggcgttac gctctggata aaccaggcgc gaacaatcgc ttccggacgg
    17941 ctctggaaca gtgcctgaca tgcattttcg ccgtagacgc gggtttcttc cgcacgctga
    18001 cgacgcaaaa cttctggatc aatataactt ttaccactga tgccaccgtg atcggccttt
    18061 tccggcgtct catcacccgg cgcgcgggaa accgtgcgcc acggcgaatc ttcccacttg
    18121 cggtcacggg gctgactctg tttgtcatcg cgggcggggc gacggccacc gtcagcacga
    18181 gattttcctg gacgcccgcc ccctttcccg gtacgcgggt tgtgggtacg tttatcagaa
    18241 tcatcatcac tgcggacata catcactttg accttgccgc ttttaccttt catttcatcg
    18301 ttcatgcttt tctccaccag cgctgcgcga agcgcgcaga ttacccgaag tccgcgcggt
    18361 tcgccatgat ttcgtaccaa agcctgcgac tatcatacct attgaataaa acagattgtt
    18421 gtctggaaca atgtccccga taatatgtaa catattagaa acataccggc gtcgttgccg
    18481 ataagtctcc ttactcatcc cgaggttagt tatgaatacc gtttgtaccc attgtcaggc
    18541 catcaatcgc attcccgacg atcggatcga agatgcggca aaatgcggac gctgcggtca
    18601 cgacttgttt gacggagagg tgattaatgc gaccggtgaa acactcgaca aattgctgaa
    18661 ggatgatcta cctgtggtga tcgacttctg ggcaccgtgg tgcggcccct gccgtaattt
    18721 cgcaccaatt tttgaagatg tcgcgcaaga gcgtagcggt aaagtgcgct ttgtgaaagt
    18781 gaataccgaa gctgaacgtg aattgagcag tcgctttgga attcgtagta taccgacgat
    18841 catgattttc aaaaacggtc aggttgtcga catgcttaat ggcgcagtac cgaaagcgcc
    18901 gttcgacagc tggctgaacg aatctcttta atcttaccgg ggcgcatctt gtgccccgtt
    18961 ttctcctctg cgacaatggc gttttttcga cgctctctta tgaccgaaaa cgctgttctc
    19021 cagttacgcg ccgagcgtat tgcgcgcgca acacgtcctt ttcttgcccg cggtaatcgc
    19081 gttcgtcgct gccaacgctg tcttttgcca gagaaattat gtctctgttc gacaattacc
    19141 ccagcacaag caaaaagtcg cttctgtttg ctgatgttcg acaccgagcc aatgaagccc
    19201 agtaataccg ggcgtctgat tgctgatatt ttgcctgata ccgttgcgtt tcaatggtcg
    19261 cgtaccgaac cctcgcagga tttgctggat ctggtacaaa acccggacta tcaaccaatg
    19321 gtggtctttc ccgcctcgta tgctgatgag caacgggaag tgatcttcac accacctgcc
    19381 ggtaagccac cgctgtttat catgctcgat ggtacctggc cggaagctcg caagatgttt
    19441 cgtaaaagtc cgtatctgga taatcttccc gtcatttccg tcgatctttc ccggctttct
    19501 gcctatcgcc tgcgtgaagc ccaggctgaa ggccaatatt gtactgccga ggtagccatc
    19561 gcactgttag atatggccgg cgataccggg gcggcggcag ggttaggcga gcattttacc
    19621 cgctttaaaa cacgctatct ggcaggaaaa acgcaacatc tgggtagcat cacagcagaa
    19681 cagttagaaa gcgtttaaaa tcattcggtc acttctgcgg gagaccggta tgagtcagcg
    19741 aggactggaa gcactactgc gaccaaaatc gattgcggta attggcgcat cgatgaaacc
    19801 caatcgcgca ggttacctga tgatgcgtaa cctgctggcg ggaggcttta acggaccggt
    19861 actcccggtg acgccagcct ggaaagcggt gttgggtgtg ttggcctggc cggatattgc
    19921 cagcttgccc tttacacccg accttgcggt tttatgtact aatgccagcc gtaatcttgc
    19981 tcttctggaa gagctcggcg agaaaggctg taaaacctgc attattcttt ccgccccggc
    20041 atcgcaacac gaagatctcc gcgcctgcgc cctgcgccat aacatgcgcc tgctaggacc
    20101 aaacagtctg ggtttactgg ctccctggca aggtctgaat gccagctttt cgcctgtgcc
    20161 gattaaacgc ggcaagctgg cgtttatttc acaatcggct gccgtctcca acaccatcct
    20221 cgactgggcg caacagcgtg agatgggctt ttcctacttt attgcgctcg gcgacagcct
    20281 ggatatcgac gttgatgaat tgcttgacta tctggcacgc gacagtaaaa ccagcgccat
    20341 cctgctctat ctcgaacagt taagcgacgc gcgacgcttt gtttcggcgg cccgtagtgc
    20401 ctctcgcaac aaaccgattc tggtgattaa aagcggacgt agcccggcgg ctcaacgtct
    20461 gttgaacacc acggcaggaa tggatccggc gtgggatgcg gctattcagc gtgccggttt
    20521 gttgcgggta caggacaccc acgaactgtt ttcggcggtg gaaaccctta gccatatgcg
    20581 cccgctgcgt ggcgaccggt tgatgattat cagcaacggt gctgcgcctg ccgcgctggc
    20641 gcttgatgcc ttatggtcac gcaatggcaa gctggcaaca ctaagcgaag aaacctgcca
    20701 gaaactgcgc gatgcactgc caggacatgt ggcaatctct aacccgctcg atctgcgcga
    20761 tgacgccagc agtgagcact atattaaaac gctggacatt ctgctccaca gccaggattt
    20821 tgatgcgctg atggttattc attcgcccag cgccgctgct cccgcaacag aaagcgcgca
    20881 agtattaatt gaagcggtaa agcatcatcc ccgcagcaag tatgtttctc tgctgacgaa
    20941 ctggtgtggc gaacattcct cgcaagaggc acgacgatta ttcagcgaag ccgggctgcc
    21001 gacctaccgt accccggaag gaaccatcac tgcttttatg catatggtgg agtaccggcg
    21061 taatcagaag caactacgcg aaacgccggc gttgcccagc aatctgacct ccaataccgc
    21121 agaagcgcat cttctgttgc aacaggcgat tgccgaagga gcaacgtcac tcgataccca
    21181 tgaagttcag cctatcctgc aagcatatgg catgaacacg ctccccacct ggattgccag
    21241 cgatagcacc gaagcggtgc atattgccga acagattggt tatccggtgg cgctgaaatt
    21301 gcgttcgccg gatattccac ataaatcgga agttcagggc gtcatgctgt acctgcgtac
    21361 agccaatgaa gtccagcaag cggcaaacgc cattttcgat cgcgtaaaaa tggcctggcc
    21421 gcaggcacgg gtccacggcc tgttggtgca aagtatggct aaccgtgctg gtgctcagga
    21481 gttgcgggtt gtggttgagc acgatccggt tttcgggccg ttgatcatgc tgggcgaagg
    21541 cggtgtggag tggcgtcctg aagatcaagc cgtcgtcgcg ttaccgccgc tgaacatgaa
    21601 cctggcccgc tatctggtta ttcaggggat caaaagtaaa aagattcgtg cacgcagtgc
    21661 gctacgccca ttggatgttg caggcttgag ccagcttctg gtgcaggttt ccaacttgat
    21721 tgtcgattgc ccggaaattc agcgtctgga tattcatcct ttgctggctt ctggcagtga
    21781 atttaccgcg ctggatgtca cgctggatat ctcgccgttt gaaggcgata acgagagtcg
    21841 gctggcagtg cgcccttatc cgcatcagct ggaagaatgg gtagaattga aaaacggtga
    21901 acgctgcttg ttccgcccga ttttgccaga agatgagcca caacttcagc aattcatttc
    21961 gcgagtcacc aaagaagatc tttattaccg ctactttagc gagatcaacg aatttactca
    22021 tgaagattta gccaacatga cgcagatcga ctacgatcgg gaaatggcgt ttgtagcggt
    22081 acgacgtatt gatcaaacgg aagagatcct cggcgtcacg cgtgcgatct ccgaccctga
    22141 taacatcgat gccgaatttg ctgtgctggt tcgctcggat ctcaaagggt taggcttagg
    22201 tcgacgctta atggaaaagt tgattaccta tacgcgagat cacggactac aacgtctgaa
    22261 tggtattacg atgccaaaca atcgtggcat ggtggcgcta gcccgcaagc tcgggtttaa
    22321 cgttgatatc cagctcgaag aggggatcgt tgggcttacg ctaaatcttg cccaacgcga
    22381 ggaatcatga gtaaggtact ggaaatgttg accacttaat cgggactggt gttattattg
    22441 cccgcttatg tcgtctgcat tgcacagagg acccttcaat gaacagagaa gaaatgcact
    22501 gtgatgttgt caaaatttaa gcgtaataaa catcaacaac accttgccca actacccaag
    22561 atttctcaat cagttgatga tgtcgatttc ttttacgctc ccgccgactt ccgggagacg
    22621 ctgctggaaa aaatagccag cgcgaagcag cgcatttgca ttgtcgccct gtatctcgaa
    22681 caggatgacg gtggcaaagg cattctgaac gcgttgtatg aagctaaaag gcagcgtccg
    22741 gaactggatg tgcgggtgct ggtcgactgg catcgtgcac aacgtggacg cattggcgct
    22801 gcggcatcta acactaacgc tgactggtac tgccgcatgg cgcaggaaaa tccgggcgta
    22861 gatgttccgg tttatggcgt tccaatcaat acccgtgaag cccttggtgt tctgcacttt
    22921 aaaggcttta tcatcgacga tagcgtactt tatagcggtg ccagcctgaa cgatgtttac
    22981 ctgcatcagc acgataaata tcgctacgac cgttatcatc tgatccgtaa ccgtaagatg
    23041 tcagacatta tgtttgaatg ggttacacag aatattatga atggccgcgg cgttaatcgt
    23101 ctggatgatg ttaatcggcc aaaaagcccg gaaatcaaga acgatattcg tctgttccgc
    23161 caggagctgc gtgatgccgc ttatcatttc cagggcgatg ccgacaacga tcagctttcc
    23221 gtaacgccgc tagtggggct ggggaaatcg agtctgttga acaagaccat tttccatctt
    23281 atgccttgtg ccgagcagaa actaaccatc tgtacgccat acttcaacct gccagcaatc
    23341 cttgtgcgca atattatcca gttgctgcgc gaagggaaaa aggtcgaaat tattgttggt
    23401 gataaaaccg cgaacgactt ctacattccg gaagatgaac ctttcaagat aattggcgca
    23461 ttgccttatc tctatgagat caatctgcgt cgtttcctga gccgtttgca gtattacgtc
    23521 aatactgacc agctagtggt tcggttatgg aaagatgacg acaacaccta tcacctaaaa
    23581 gggatgtggg ttgatgataa gtggatgttg atcaccggta ataacctgaa cccgcgcgcc
    23641 tggcgtctgg atctggaaaa cgccattttg atccacgatc cacaactgga gcttgcgccg
    23701 cagcgagaga aagaactgga gctgatccgc gagcatacca ccatcgttaa gcactatcgc
    23761 gatctgcaaa gtattgccga ttatccggtg aaggttcgta aactcatccg ccgtttgcgc
    23821 cgtatccgca tcgaccgatt aattagccgc atcctgtaat cacaaccccg tcctgtacgg
    23881 ggtttgtttt gtggaggcca cgttttgcgt attctttttg tctgttcgct attgttactt
    23941 tctggatgca gccatatggc taacgatagc tggagcgggc aggataaagc tcaacacttt
    24001 attgcctcgg cgatgctttc cgccgccgga aatgaatatt cacagcatca ggggatgagc
    24061 cgggatcgca gtgccatgtt tggattgatg ctctctgtca gtttgggggc gtcaaaagag
    24121 ctttgggata gccgccccga agggagcggc tggagctgga aggatttggc ctgggatgtc
    24181 gccggtgcaa gcaccggcta taccgtctgg caactgaccc gtcactaaag acgcatcccc
    24241 ttccctttgc gatgtagcat caaagaaacc agaaacgcca ccacggccat caaggtcaca
    24301 taccagaaga aggctgtttc cattcctatt gatttcagcg acaacgctac gtactccgcc
    24361 gaaccaccaa atatagcatt agcgaccgca tatgacaggc caacgcctaa tgcgcgaacc
    24421 tgtgccggga acatctcagc cttcagtatt ccactgattg atgtataaaa actcactatc
    24481 agcagggcac acatcaccag accaaaagcg gcataaggcg aggaaacgtt ttgcaatgct
    24541 gagagaatag gaacggtaaa aatggctgcc agcgaaccga aacataacat tgaggtacgg
    24601 cgaccaatct tatccgacag cgcgccaatg agtggttgaa taagcatgaa tacaaacaat
    24661 gcggcagtca taatgccact cgccacgttg gcatgcattc ccgcagtatt taccagatac
    24721 ttctgcatat aagtggtgaa ggtatagaaa caaagggagc ccgcagcggt aaaaccgaga
    24781 accatgatga atgcacggcg attgcgccat aatcctttca gagatccagc ttcttttaaa
    24841 gcgcgcgttt cttgttgcga agtttcatct aactgacgac gtaaccacaa cgccacaaca
    24901 gctaacacag ctcctaacgc gaaaggaata cgccatcccc actctctgag tgcagcgtct
    24961 tccatggtgt gttgtaaaac cacgacaacc agtagggcta gcagttgtcc gccgatcaac
    25021 gtcacatact gaaatgatgc gtaaaaacct ttgcgccctt caacggcaac ttcactcata
    25081 taggtggcgc tggtgccata ttctccgcca acagataatc cctgaaataa acgcgcgaga
    25141 agcaataatg ccggagccca cgtacctata gtttcataac ctgggaggca ggcgataacc
    25201 agcgatccga aacacatcat acacaccgat aacagcatcg attttttgcg accatgttta
    25261 tcggctatgc ggccaaatag ccaaccgcct attgggcgca tcaggaatcc cgcagcaaaa
    25321 acacctgctg tttgtagtag ttgagtcgtc gtgttcccgg aagggaagaa aatgtgggca
    25381 aagtagagtg aacagaacga gtagacatag aaatcgaacc actcgaccag attacctgaa
    25441 gaggccccca caatcgccca aatgcggcga cgagtatcac tacttgtcag tttgctgtct
    25501 gccgttacag tactttcagc catgccatta tgtctcctgc cgtaatccga tgcttttgtc
    25561 ggtcgctttt atttatttct tttggtaaag aaaatgttat acatttgttg cgtatcatta
    25621 tgcaacctta accatgaatt tagttagcag gaaaatggtt attgaggagc ttaaggataa
    25681 atttctggta aggaggacac gtatggaagt aggcaagttg gggaaaccgc atccgttgct
    25741 gaatctggca tatgtgggag tataagacgc gcagcgtcgc atcaggcatt tttttctgcg
    25801 ccaacgcaaa aaggccatcg tgactgatgg cctttcgctt aatttgatgc ctggcagttc
    25861 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag
    25921 ttcggcatgg ggtcaggtgg gaccaccgcg ctacggccgc caggcaaatt ctgttttatc
    25981 agaccgcttc tgcgttctga tttaatctgt atcaggctga aaatctttct ctcatccgcc
    26041 aaaaca
//
LOCUS       DAZFRN010000063        25741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFRN010000063 DAZFRN010000000
VERSION     DAZFRN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.063"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            151..1404
                     /gene="amiC"
                     /locus_tag="ACHKUB_002499"
     CDS             151..1404
                     /gene="amiC"
                     /locus_tag="ACHKUB_002499"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO6655505.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(1479..1555)
                     /locus_tag="ACHKUB_002500"
     tRNA            complement(1479..1555)
                     /locus_tag="ACHKUB_002500"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1519..1521),aa:Met,seq:cat)
     gene            1764..2861
                     /gene="mltA"
                     /locus_tag="ACHKUB_002501"
     CDS             1764..2861
                     /gene="mltA"
                     /locus_tag="ACHKUB_002501"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO6655506.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            2938..3744
                     /gene="tcdA"
                     /locus_tag="ACHKUB_002502"
     CDS             2938..3744
                     /gene="tcdA"
                     /locus_tag="ACHKUB_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO6655507.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADVHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3795..4238)
                     /gene="csdE"
                     /locus_tag="ACHKUB_002503"
     CDS             complement(3795..4238)
                     /gene="csdE"
                     /locus_tag="ACHKUB_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO6655508.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(4238..5443)
                     /gene="csdA"
                     /locus_tag="ACHKUB_002504"
     CDS             complement(4238..5443)
                     /gene="csdA"
                     /locus_tag="ACHKUB_002504"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO6655509.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5635..5862
                     /gene="ygdI"
                     /locus_tag="ACHKUB_002505"
     CDS             5635..5862
                     /gene="ygdI"
                     /locus_tag="ACHKUB_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO6655510.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            6213..7130
                     /gene="gcvA"
                     /locus_tag="ACHKUB_002506"
     CDS             6213..7130
                     /gene="gcvA"
                     /locus_tag="ACHKUB_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO6655511.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            7149..7544
                     /gene="ygdD"
                     /locus_tag="ACHKUB_002507"
     CDS             7149..7544
                     /gene="ygdD"
                     /locus_tag="ACHKUB_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO6655512.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7537..8637
                     /gene="rlmM"
                     /locus_tag="ACHKUB_002508"
     CDS             7537..8637
                     /gene="rlmM"
                     /locus_tag="ACHKUB_002508"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO6655513.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8681..9412)
                     /gene="fucR"
                     /locus_tag="ACHKUB_002509"
     CDS             complement(8681..9412)
                     /gene="fucR"
                     /locus_tag="ACHKUB_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO6655514.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(9470..9892)
                     /gene="fucU"
                     /locus_tag="ACHKUB_002510"
     CDS             complement(9470..9892)
                     /gene="fucU"
                     /locus_tag="ACHKUB_002510"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO6655515.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(9894..11312)
                     /gene="fucK"
                     /locus_tag="ACHKUB_002511"
     CDS             complement(9894..11312)
                     /gene="fucK"
                     /locus_tag="ACHKUB_002511"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO6655516.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11421..13196)
                     /gene="fucI"
                     /locus_tag="ACHKUB_002512"
     CDS             complement(11421..13196)
                     /gene="fucI"
                     /locus_tag="ACHKUB_002512"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO6655517.1"
                     /translation="MKINTLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            13666..15165
                     /locus_tag="ACHKUB_002513"
     CDS             13666..15165
                     /locus_tag="ACHKUB_002513"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6655518.1"
                     /translation="MSETFLQMKHITKRFPGVLALNDVQFTLRRGEVHALLGENGAGK
                     STLMKILSGVYQPDEGEIIFEDKPVSFSDPLSAQNVGITIIHQEFNLFPELTVEENIF
                     IGREFCKKNRWRLDEKQQRQATIEILQKLNLAIKPDTLVADLTVAQQQMVEIAKAISV
                     NARILIMDEPTAALTETEIESLFRVTRLLKEQGTGIVYISHRLEELALIADRATVMRD
                     GQYISTVDYECVKISDLIAMMVGRDLGNIYPRREALQQRIPVLEVNGLTRKGVLNDIN
                     FTLYRGEILGFAGLMGAGRTELARAIFGADSIDSGTLKLNGKETVIKDISDAIQQGIS
                     YLTEDRKKEGLALNLSVERNIMLGNYPEYSDRFGNIDSRRCQQTSEEQVKALRIKTPN
                     LEQAALNLSGGNQQKIIIARWVCKDTDILIFDEPTRGIDVGAKLEIYELMNRLVAKGK
                     SIIMISSELPEVLGMCDRILVMRSGRITGELSAKEATQEKIMQYATLED"
     gene            15169..16152
                     /locus_tag="ACHKUB_002514"
     CDS             15169..16152
                     /locus_tag="ACHKUB_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6655519.1"
                     /translation="MTISVTSPDSNNKKIKINKELLMRLAPLFSLIILVLFFSFSSPF
                     FFNTENIMTIALQTSVIGIMAIGVTFVIITAGIDLSLGSVVAFSGVAVGICATLGLPL
                     PVCIIAGVLAGGLCGYVNGLLVTKMTIPPFIATLGLMMSVRGINMVMTDGRAIYFADY
                     PMFKTLAQGRLFDVLPYPVFYLVIVALVGAYILKKTVIGRYVYAVGSNEVAAHLSGIK
                     VQRVKIFVYAFCGLLTGIAGVILASRLNSGQPTVGVGYELEAIAAVVIGGTSLMGGIG
                     TIGGTIIGAFIMSVLKNGLNLMGVSQFWQMVAMGVVVVAAVYLDTLRKKIR"
     gene            16226..17176
                     /locus_tag="ACHKUB_002515"
     CDS             16226..17176
                     /locus_tag="ACHKUB_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6655520.1"
                     /translation="MKTRHFIYALSLLACVTSSALAKDLNLPVVSKGFQHEFWQTVKM
                     GTEAAAKELGDKTSYVGPADETQIAEQIQLVENAMAQKPNGLLLAALDANALAPLVET
                     ANSRGIKVVTFDSGINSDIPVSFVATNNRKAGAQAADALAAQVNNKGKVGIIAHVAGT
                     SSAIERSEGFMERMKEKYPDIKVLPVQYSDGDPQKAMDKTIDMIQANPDLAGIYGTNE
                     GSTLGVANAIDSQNLKGKVKVIGFDSTEAIINFLKNGVIQGFVVQDAYQIGYQGIKTL
                     NAALSGQAVEKEIDIPVKFVNAENINTPEIDKLLHPFGKK"
     gene            17229..18809
                     /locus_tag="ACHKUB_002516"
     CDS             17229..18809
                     /locus_tag="ACHKUB_002516"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO6655521.1"
                     /translation="MHNGYFLGVDVGSASVRAGVYNASGHRLAFATAPVSQFRPGGER
                     VEQSSAEIWQQVCKTVKEATALAGIPISAIRSLGFDATCSLVVLDEQGQGLAVSSGGS
                     ANHDIIMWMDHRATVETAQINATKDPVLRYVGGEVSVEMELPKLRWLKTHLPQTWQAA
                     HRFFDLADFLVWKATGRDVAGLCTLTCKWNYLAHEQRFSHSLLESVDLTDMLERIPAE
                     ILPPGAAVGTLTAKAAEELGLTTNVIVASGLIDAHAGGMALAGAHPSGTLALISGTSN
                     CHMLCSEKEIFTPGVWGPYWSAMLPNYWLTEGGQSAAGALVEWTLQESGASANLFHKA
                     QQRGCHPIQLINEWVAALEANESEPTRNLHVLADHHGNRSPRARPDARGSVYGLTLER
                     GENQLARLYLATLQAIAFGTRHIIETLRENGHTITRLTLCGGATHNPLWLREYADVTG
                     CDIHLMQEEDAVTLGAAISGAVASGAWGDFTSACKAMVEAGEVIQVNPQRREFLERKY
                     RVYLTLWEQQQAVNQLMQ"
     gene            18874..19872
                     /locus_tag="ACHKUB_002517"
     CDS             18874..19872
                     /locus_tag="ACHKUB_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6655522.1"
                     /translation="MAKTVEQIASDLNLSVTTVRLVLNGKAEQYRISVKTQTRINEYV
                     ERYGYIINHSARSLKLNKTDTLGLIVPNISNVFFATLAEKLEQRCRRSGYQLTISCTY
                     DDVDYENKLTKAFIARNVDGLFIVPSTLENQQHHLRKIRKPMVLLDRDFKYTDNALVE
                     SNNISGGEKLTQNILEAGQAPVWFLLGDAGLPSISDRLTGYLNALEKKGITHRDWVRE
                     GPVNTPEGGYVIMEKLIEEQGCPQAFIASSLPVLEGAVRAIRDRLGAVPPEINIGTFD
                     EHPMLGFLANNVWSMQQDENAWAEKAFEMMLSAIEERPVKKTVKVEMKLIKRIRKK"
     gene            20005..20652
                     /gene="fucA"
                     /locus_tag="ACHKUB_002518"
     CDS             20005..20652
                     /gene="fucA"
                     /locus_tag="ACHKUB_002518"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO6655523.1"
                     /translation="MERTRLSREIIETCLEMTRLGLNQGTAGNVSTRFENGMLITPSG
                     IPYERMTENMIVYVDGNGKYEEGKIPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            20680..21828
                     /gene="fucO"
                     /locus_tag="ACHKUB_002519"
     CDS             20680..21828
                     /gene="fucO"
                     /locus_tag="ACHKUB_002519"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO6655524.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(21883..22638)
                     /gene="xni"
                     /locus_tag="ACHKUB_002520"
     CDS             complement(21883..22638)
                     /gene="xni"
                     /locus_tag="ACHKUB_002520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO6655525.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(22750..24117)
                     /gene="sdaB"
                     /locus_tag="ACHKUB_002521"
     CDS             complement(22750..24117)
                     /gene="sdaB"
                     /locus_tag="ACHKUB_002521"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO6655526.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(24175..25464)
                     /gene="sdaC"
                     /locus_tag="ACHKUB_002522"
     CDS             complement(24175..25464)
                     /gene="sdaC"
                     /locus_tag="ACHKUB_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO6655527.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         6399 a         6157 c         6802 g         6383 t
ORIGIN      
        1 gagagcgact tttttatcag taatgccgtg aatataaaaa gaatgtttac cgtttattag
       61 atgacagatt atgcgtcttt cgctaaagtt tccggtcaaa ttagtcgttt acttgttaca
      121 cagcttagat ttcttttgct cgggagaggc atgtcaggat ccaacactgc aatcagccgt
      181 cgtcgtttac tgcaaggcgc gggcgctatg tggctattga gcgtaagtca ggtcagcctg
      241 gctgcggtta gccaggtcgt ggcggtgcgc gtctggcctg cgtccagcta cacccgcgtg
      301 acggtagaat caaatcgtca gctgaaatat aagcagttcg cgttgagtaa cccggaacgc
      361 gtggtggtgg atatcgaaga tgtaaacctg aactcggtac tcaaggggat ggcggcacaa
      421 attcgtgcag acgacccgtt catcaagtcg gcgcgcgtcg ggcaatttga cccgcagacc
      481 gtacggatgg tttttgaatt aaagcaaaac gtaaaaccgc agctgtttgc ccttgcgccg
      541 gtcgccgggt ttaaagagcg tctggtgatg gacctctatc cggccaatgc acaggatatg
      601 caggacccgc tgctggcgct gctggaggat tacaacaaag gcgacctcga aaagcaggtg
      661 ccgccagcac aaagtggtcc acaaccgggt aaagcagggc gcgatcgtcc tattgttatt
      721 atgcttgacc ctggccacgg cggcgaagac tccggtgcgg tggggaaata caaaacgcgc
      781 gaaaaagacg tggtattgca aatagctcgc cgtctgcgct ctctgatcga gaaagagggc
      841 aatatgaagg tgtacatgac gcgcaatgaa gacatcttca ttccgttgca agtgcgcgta
      901 gcaaaagccc agaaacagcg tgccgacctg tttgtctcta tccatgccga cgcctttacc
      961 agtcgccagc cgagcggttc ctcagtgttt gcgctctcaa ccaaaggcgc aaccagtact
     1021 gcggcaaaat atctggcaca gacccagaac gcctcggact tgattggtgg cgtgagcaaa
     1081 agcggtgacc gctatgtcga ccacaccatg ttcgatatgg tgcagtcgct gaccattgcc
     1141 gacagcctta agtttggtaa agcggtgctg aataagctcg gtaaaatcaa caagctgcat
     1201 aaaaatcagg ttgaacaggc cgggtttgcc gtgctgaaag cgcctgatat tccctccatt
     1261 ctggtcgaaa cggcgtttat cagtaacgtt gaggaagagc gtaaactgaa aacggcgact
     1321 ttccagcagg aagttgcgga gtctattctt gcgggaatta aagcgtattt tgccgatggg
     1381 gcgacgctgg cgagaagggg atgataaaaa ggcgctgaat ggcgcttttt ttatgggaga
     1441 gcagaaacaa aaaaacaccc gttagggtgt tcaataattg gttgcggggg ccggatttga
     1501 accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc ccgcgtccgt
     1561 ggatgcgcac tatactccgc tcgcgttttc atgcaacctt ttttttaccc cgatcacgga
     1621 tttggcatga ttttgaacaa aaacaaatca ctaaagccta ttttttgtgc aaaatttgcg
     1681 tcagggagag gctgctcatt tcattaaacg cggcggtttg ttatcttcgt tgcgccttat
     1741 tttttaacct gaagaagaga acaatgaaag gacgttgggt aaagtacctt cttatgggca
     1801 cggttgtggc aatgcttgcc gcctgctctt ccaaaccaac cgatcgcgga cagcaatata
     1861 aagacgggaa atttacccag cctttctctc tggtgaacca gccagatgcc gttggcgcgc
     1921 cgattaacgc cggtgatttt gccgagcaaa ttaaccatat ccgtaattcg tcaccgcgtc
     1981 tgtatggcaa ccagagtaat gtttataacg cggtgcaaga gtggctgcgc gcaggcggtg
     2041 atacccgcaa tatgcgtcag ttcggcattg atgcctggca gatggaaggt gccgacaact
     2101 atggtaacgt gcagtttaca ggctattaca cgccggtaat tcaggcgcgc catacccgcc
     2161 agggcgagtt ccagtatccg atttaccgta tgccgccaaa acgtggtcgt ctgccgtctc
     2221 gtgcggagat ctacgcaggg gcgctgagtg ataaatatat tctcgcttac agcaactccc
     2281 tgatggataa cttcattatg gatgtgcagg gtagcgggta tatcgacttt ggtgatggca
     2341 gtccgcttaa ctttttcagc tatgcaggga aaaacggtca tgcctatcgc agcattggta
     2401 aggtgctgat cgaccgtggc gaagtgaaaa aagaagatat gtcgatgcag gcgattcgtc
     2461 attggggcga aacacacagt gaagccgagg ttcgcgagct gctggaacag aacccgtctt
     2521 tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg ggcaagtgcg gtgccgctgg
     2581 ttggccgtgc ttctgttgcc tccgatcgtt caattattcc gccaggtact accttgctgg
     2641 cagaagtgcc gctgctggat aataacggca aatttaacgg tcagtacgaa ttgcgtctga
     2701 tggtggcgct ggatgtcggt ggcgcaatca aaggccaaca cttcgatatc tatcaaggga
     2761 tcgggccgga agccggacat cgcgcaggtt ggtacaacca ctatggacgt gtctgggtgc
     2821 tgaaaaccgc cccgggcgca ggtaacgtct ttagcggctg atgtgttatt ctgtataccg
     2881 tttttacgga tacgccaggg tgagggataa cctcaccctt tttaattctg aggtgctatg
     2941 tctgtggtaa ttagtgatgc atggcgtcag cgttttggtg gcacagcgcg tctgtatggt
     3001 gaaaaagcgt tgcaactgtt tgctgacgtg catatttgtg tggttggtat cggtggtgtc
     3061 ggttcctggg cggcggaagc gctggcgcgc acggggattg gcgcaatcac gcttatcgat
     3121 atggatgatg tgtgcgtcac caataccaat cggcaaattc acgctctgcg cgataacgtc
     3181 gggctggcaa aagcggaagt catggcggag cgtattcgcc agattaaccc ggagtgccgt
     3241 gtaacggtgg tggatgattt cgtgacgccg gataacgtag cgcagtatat gagcgtgggt
     3301 tattcgtacg tgattgatgc cattgatagt gtacggccca aagcggcgct gattgcttat
     3361 tgtcggcgca ataaaatccc gctggtcacg accggtggcg cgggtgggca gattgacccg
     3421 acgcagattc aggttaccga tctggcgaaa acgattcagg atccgctggc ggcgaagttg
     3481 cgcgagcgcc tgaaaagcga ttttggcgta gtgaaaaaca gtaaaggtaa gctcggcgtc
     3541 gattgcgtgt tttctactga agcgctggtg tacccgcagt cagacggtac tgtgtgtgcg
     3601 atgaaagcca cggcagaagg gccgaagcgg atggattgtg catctggatt tggcgcggca
     3661 acgatggtga ccgccacctt tgggtttgtt gcggtttctc atgcgctgaa gaagatgatg
     3721 gcgaaagcgg cgcgtcaggg ttaaacaatc aggtcggatg cgacgcgaac gtcttatccg
     3781 acctggttct gtgcttaaac ctgcttcgcc gcagcgataa tcgcctcgct taacgcattc
     3841 aacccctggc tgcgtgaggc gctaagctgt gcacgcaatc ccagctcatc aaacaatgcc
     3901 agcggcgact gtgcctgcaa ttcggcggcg gttttcccct caacggcagt caacaacacc
     3961 gccaacaggc cgcgcacaat gcgcccttcg ctatcgccaa agaagtgcat tttaccgttt
     4021 tcagtcacgg tatatcccag ccagacgcgg ttttcgcatc cggcaatctc tttagcctgc
     4081 gcttttaact cgtctggcaa agccggaagc tgtttcccca gcatgatcaa ctggcgatat
     4141 ttatcttccc attgcgacaa cggagcgaag gtattgcgta acgtttctgc ggttacggtt
     4201 gtgccgaacg gatgtccggc gaattgcggg tttgtcatta atccaccaat aattccagcg
     4261 cgcggtcaac ggcattcacc agcgcatcca catcactctt tgtattatat ggcgcaaatg
     4321 aggcgcgcag tgtgccggtt acgcctaatt ctgccagtag cggctgagcg caatgttgcc
     4381 cggcccgcaa ggcaataccg tactccgcca gcagcgtcac catatcgcta tgatgaacgc
     4441 cagcaaaatc aaaggccagc aggctggaat cctggcagcg gaatgaacga aagcctggac
     4501 gtttcgccag cgcttcttct gcaagcgttg ctaagctacg gctccagctt tcggcctggt
     4561 tgatatcgta atctgccagc cattccagcg ccgcgcttaa tcctatgacg ccagcgacat
     4621 ttggcgttcc tgcttccagt ttccacggcg cagattgagt ggtgaaaccg tcaaaactca
     4681 cttcgtgaat cattttgccg ccacccagcc agggcgacat cgcttccagc agttctgatt
     4741 tgccatacag cacgccgata cccgtcgggc catacagttt gtgaccagag aaagcataga
     4801 aatcgatatc tagttgctga acatctgcgg ggaaatgcac cgccccctga gcaccatcaa
     4861 ccatcaccac catcccggct gaatgagcaa aggtaatcgc tcgcgccaga tccgggcaac
     4921 cgccagtaac gttcgacatc tgacccaacg ccagaatccg actacgggga gtaatcagtt
     4981 ctggcaacaa atcgacatcc ggcagtcgct gcgcattaag cggcaatttc accactttgg
     5041 ctccagtttg ttgggcgacc atcagccagg ggacgaggtt agcgtggtgt tctgccacgc
     5101 tgacaataat ctcatcgccc ggttgcagac gcggacgcgc atagcattgt gccaccatgt
     5161 tgatggattc agtggtgccg cgcgtccaga cgatagtttt atcatccggt gcattcagta
     5221 attgcgccac tttttcccgc gcagcttcat aacgcgcggt caggcgttgg gcttcggcaa
     5281 actggctgcg atggacgttt ccggcgctca gactgtaaaa ctgtcgggtg gcttcaacca
     5341 cggcttcagg tttaagcgcg gtcgcggcgc tgtcgagata gacgcccgca tcctgtagtg
     5401 cgggaaactg ggcgcgaaac tgcgcgggat taaaaacgtt catggtactc ctcggcttga
     5461 tgcaccgatc gtgacgcaaa attcactttg atacaaggag agtgctaacc atcagaatta
     5521 tctggatatc ctggcgaagc cgcgtaagta acatatgctg aatagtgtta ggcgttaatt
     5581 ttagcctgtt aatcaatact attaagattt attaatcgct ttaaggagaa gatgatgaaa
     5641 aagactgccg caattatttc tgcctgtatg ctgacttttg ccctgagcgc ctgttccggt
     5701 tcgaactatg tgatgcacac caatgacgga cgtaccatcg tctctgacgg caaaccacag
     5761 actgataacg ataccggtat gatttcgtat aaagacgcta atggcaacaa acagcagatc
     5821 aaccgtactg acgtgaaaga gatggtcgaa ctggatcagt aattcgcgat cgcaaggtaa
     5881 aaaaaagcac cgcaattagg cggtgctaca ttaatcacta tggacagaca gggtaaatgt
     5941 acaggaagtg aaaaaaggta gctttgctac catggtctga atcgcagacc aattgcaaac
     6001 acaacaacac aacatcacaa ccgtaagcca aaagttcacc agaacacgca ttccgataaa
     6061 acttttcgtt ccggctcagg aagtgccgcc actataggta tttgctggta gaagctcaac
     6121 ggacaattta taatggctca gattaaaaaa actaataggt tacatagtgt gatctaattg
     6181 ttaaattcat ttaacatcaa agtttaaaag ccatgtctaa acgattacca ccgctaaatg
     6241 ccttacgagt ttttgatgcc gcagcacgcc atttaagttt cactcgcgca gcagaagagc
     6301 tttttgtgac ccaggccgca gtaagtcatc aaatcaagtc tcttgaggat tttttggggc
     6361 taaaactgtt ccgccgccgt aatcgttcac tcctgctgac cgaggaaggg caaagctatt
     6421 tcctcgatat caaagagata ttttcgcaat taaccgaagc gacgcgtaaa ctccaggccc
     6481 gtagcgccaa gggggcgttg acggtcagtt tactccccag tttcgccatt cattggttgg
     6541 ttccgcgact ttccagcttt aattcagctt atccgggaat tgacgttcga atccaggcgg
     6601 ttgatcgtca ggaagataag ctggcggatg atgttgatgt ggcgatattt tatggtcggg
     6661 gcaactggcc ggggctacgg gtggaaaaac tgtacgccga atatttattg ccggtgtgtt
     6721 cgccgctact gctgacaggc gaaaaaccct tgaagacccc ggaagatctg gctaaacata
     6781 cgttattaca tgatgcgtca cgccgtgact ggcagacata tacccgacag ttggggttaa
     6841 atcatatcaa cgttcagcaa gggccaattt ttagtcatag cgccatggtg ctgcaagcgg
     6901 ctattcacgg gcaaggagtg gcgctggcaa ataacgtgat ggcgcaatct gaaattgaag
     6961 ccggacgtct tgtttgcccg tttaatgatg ttctggtcag taaaaacgct ttttatctgg
     7021 tttgtcatga cagccaggca gaactgggta aaatagccgc ctttcgccaa tggatcctgg
     7081 cgaaagccgc tgctgaacaa gaaaaattcc gctttcgtta tgaacaataa tttacgtagg
     7141 gtacgaccat gaccagccgt tttatgctga ttttcgccgc cattagcggc ttcatttttg
     7201 tggctctggg cgcttttggc gcgcatgtgt taagtaaaac catgggggcc gttgagatgg
     7261 gctggatcca gaccggcctc gaataccagg cgtttcatac gctggcgatc ttaggtctgg
     7321 cggtggcaat gcagcgtcgc atcagtatct ggttttactg gagtagcgtt ttcctcgcgt
     7381 taggcacggt gttgttcagc ggcagccttt attgcctggc gctgtcccat ctgcgtttgt
     7441 gggcgtttgt cactccggtt ggcggcgtga gcttcctcgc gggctgggcg ttaatgttag
     7501 ttggtgctat ccgtttaaag cgcaagggcg taagtcatga ataaggttgt attgctgtgc
     7561 cgtccgggct ttgaaaaaga gtgcgccgca gaaattaccg ataaagccgg ccagcgggaa
     7621 attttcggtt ttgcccgcgt gaaagagaat gcgggttatg tcatttatga atgttatcaa
     7681 cctgatgatg gcgataagtt aatccgtgag ctgccgttca gttcattaat ttttgcccgc
     7741 cagtggtttg tggtggggga gctcctgcag catttgccgc cagaagatcg tattaccccc
     7801 attgtcggca tgttacaggg tgtagtagag aagggcggtg aactgcgtgt tgaagttgcc
     7861 gataccaacg aaagcaaaga gttgctgaaa ttctgccgta agttcactgt tccgctacgc
     7921 gctgccttgc gcgatgccgg ggtgctggcg aactatgaaa cgccgaagcg tccggttgtg
     7981 catgtattct ttattgcacc aggctgctgc tataccggtt actcatacag caacaataat
     8041 tcgccgttct atatgggcat tccgcgcctg aaatttccgg cagatgcgcc gagtcgttcc
     8101 acgctcaaac tggaagaggc atttcatgtg tttattcctg cggatgagtg ggatgaacgc
     8161 ctggcgaacg ggatgtgggc ggtggattta ggcgcttgcc ctggcggctg gacctaccaa
     8221 ctggtgaagc gcaacatgtg ggtttattcc gtcgacaacg gcccgatggc gcaaagtctg
     8281 atggataccg gacaggtgac gtggctgcgg gaagacggtt tcaaattccg tccgacgcgc
     8341 agcaatatct cctggatggt atgcgatatg gttgaaaaac cggcgaaagt tgcggcattg
     8401 atggcgcagt ggctggttaa tggctggtgc cgtgaaacca ttttcaacct caaactgccg
     8461 atgaaaaaac gctacgaaga agtgtcacac aatctggcgt atattcaggc acagcttgat
     8521 gaacatggca taaatgctca gattcaggcg cggcagttgt atcacgatcg cgaagaagtg
     8581 acggtgcacg tccgccgcat ctgggctgcg gtgggtggtc gtcgcgacga gcgataacag
     8641 gcaaggccgg ggcgtcgtct ccggcctgct accctttccg tcaggctgtt accaaagaag
     8701 ttgcaacctg gcgctcatca gaaataatgt gcgttacctc atccagatgc ccgatgcggg
     8761 cttcccctga acgattaaat ttacttttat caatcaataa caacgattgc gcggcacgtt
     8821 ttaatagcat cgatttgtaa tcagcgttga tcgcattgga gtcccacagt gcgccgctgc
     8881 tatcgatccc ttcacaagaa aaaataaaca gatcgatttc cagcgattta agttgggaaa
     8941 tcagcgaggg attgacgtaa cagccatatt tgcgctcaag tgtgccgccg gaactgatca
     9001 gttgaatgcg ttcgcgttta ccgagttcat ggcaaatcgg atggctattg gtgaagacct
     9061 gaatgttgat gtcaggcaac tggcgtgcca gataccagca agttgaactg gcgtctaagg
     9121 ctatcaccat cccttcttca atccacgcga gcgcctcgcg cgcgatatct gctttatgcg
     9181 cataatggct tttcagcctg atgtgaaagg gatcgccact gtcttgattt tgacggtgga
     9241 tatatttagc gcgtccatga ttgcgcagaa ttttaccctg cgtctgtaat tcattgagat
     9301 cgcgacgaat ggtttcttta ctgaccttta gctgttcaga gagagcttcc gtggtcaggc
     9361 tggtatggtt cagcagcagg tcgactatcg cttgctggcg tgccgctttc atatttttca
     9421 ccccatcatt cgataaccgc ccgcgtcatg cgggcgtacc ggtatgagat tacggtgtta
     9481 cccctttttt taaaagaata ttcccgtact tcgctcgttc gcctgtgata acgatcgcaa
     9541 aggctttttg cgcccgttca taaaacgcaa aacgattgat gcggatgatg tcaggacacg
     9601 gggcttgtag tgaaagcgca ttacggtaac gtcgttctac ttcaggatcg agagtgtcac
     9661 cttctaccgc cgccatcatc accagcggcg gtgcataact gtccagttca aataacggga
     9721 taatcgcctg gagcaagtcg ctcaccaaca ggccatcagc gcggatcacc tgcggtccca
     9781 tcgaatgggc gggaaagtga gcatcggaaa aaataatttc atctccatgt cccatctctg
     9841 ccagcacttt caatagttcg ggagaaatta acggcgaaat tgttttcagc atttcacact
     9901 tcctctataa attcaggttc agtttgcggg tagaaataac ggtactgata atgaatctgt
     9961 gcgcgggctt cttccgggct gttaaattcc cctacgccat accaaccgaa cagcgcagct
    10021 cctgcgacgg tcgtttcggc gtcgtcgaga acttttaccg gaatatcaag catattggct
    10081 ttaatctgat tccacaatgt gttgcgactt cctccaccga ctaacaataa ttcagaggcc
    10141 ttaaagtgcc cgattttttc cagcatctgt agattgcgct gtaattgcgc agttaaccct
    10201 tccagcgccg cgcgatagaa atgcccccgc gtggtattaa gcgtcactcc ttgccagcca
    10261 gcgttctgac acgacaataa atcacactgc atttttacgc catccgcgcc aggcgcgatc
    10321 agacgagctt cttcaatcaa catttgccag ggtgtttcag ccgtccagaa cagttttctc
    10381 acccattcca gcacgccgga tgccagccat tgcatacctg ggttatacaa ccctgcctgg
    10441 ctatccagtt cgcaggtgga accggcgtac tgacttaaca gcgaagtatc aacctgggcg
    10501 ctacggacca ttaaaatttc ccatgtaccg gaagagagca cgggttcatt ttggtcggca
    10561 ccagcgccaa aaagggcgaa ctgggtatcg tgacctgcgg aaatcaccgg tatgccaacg
    10621 ggtaagccga gcattgctgc ggcgctgttc tgtagcgtac caatctgttc acccgcttcc
    10681 accagacgag ggaagagtcg gcgtggaata ccggtggctt gtaaaatttg cggactgaaa
    10741 tcgcgttgct ggatatccag catctggctg gttccggcca tcgtgatatc agtagtgaat
    10801 tcgccggtta aacggtggtt aatcagcgac gaaataaaga gccaggcgtg cgcgcgttcc
    10861 agcagttgtg gatgattttc tttcaaccac accaacttat ataacgtatt gaaactaaag
    10921 gctccgacgc cagaaatagc ctgcaaccgc tgtgcggaga tcaactgttc aatattgtcc
    10981 ataaccgctg ctgttcgcgg acatttccag ctaatgatcg gatagagcag attgccttgc
    11041 ttatctacca gagcgccatc cacaccaaag gtggtgacgg cgataccgcg gatgtggcat
    11101 tcagtcagtt cactattgat ttgccgacag caatcagcaa agcgttgcaa aatggcgtct
    11161 aaagaccact ggtgccaggt gttgttttcc atcgcgatat cgctggcatt aggcgttgag
    11221 gcgcgggcaa caatttttcc ctgccgatta accgcgatgg ccctgacatt ggtcgcgcca
    11281 cagtcgagta ccaggataac ttcttgtttc ataatcgctc ctgcaatata gccggataac
    11341 attgcttatc cggctaacca ctcttgcttg ttcaggcccg gtagccggag cgaccgggca
    11401 tcacatcagg gagtaatgta ttaacgcttg tacaacgggc cgtagttctg gcaagcgcgg
    11461 taatcctgac cttcaatatc catgccgtgc gcggcccagg cagaaggacg atatactttg
    11521 gtctcttcaa cgttgtgcat acataccggg atacgcagca tggaggcgag agtgataaag
    11581 tctgcgccaa cgtggccgat ggtcagaacc ccgtggttag cgccccagtt cgccattacc
    11641 gaatacacat ccgtaaacgg ccctttaccg gtgaggcgcg gtgcaaacca ggtggttggc
    11701 caggttgagt tggtgcgttt gttgaggatg tcatgcacat ccttcggcaa ttccacgctc
    11761 cagccttccg cgatttgcag taccggtccc aggcctttga tgatgttgac acgagtcatg
    11821 gtgaacggga cgccgccttc ggtaaggaag cgggaagagt aaccgccgcc acggaagtat
    11881 tcgtggatcg ccgggcacca ttcggtagcg gcgaggcaag cgtcagcctc ttgctgagag
    11941 atttcccagt gtggcttcat cgtcgggtta ccttcgctgt cgcgttgttt acaggaaccg
    12001 tccagcgcag cagaaccgga gttgatcaaa tggatgatgc cgtgttctgc cagtccatcg
    12061 agtttatgcc ccgttacacg ctcaattgct tctggtgacc agtaggtacg cacatcggca
    12121 aatacctgag cggtgccggt gagctggtga cccattagca ttgccacgcc gttaagactg
    12181 tcgttttcgg tcgccacgac aaagggttcg cgcacgccat tccagtcaaa tgaactgttg
    12241 aggatcgctt cggcggtgtc accattggga tattgatcgg tccagtgacg ttgcccctgg
    12301 aagcccgcag cgatggcgtt gtagccaagt gattcttcca cgcgaccaat atcggccagt
    12361 ttgctgttgc cttgcatcat gtcgcggata cacatcgcca tcagtaaact ttcgcgcaga
    12421 actgcgcggc tttgctcggc attacgttga tattgtttgt tattttcatc ttcgccatag
    12481 cggaagtttt tatcagccca ggccagcgcc atttccagtt cggcttcgtc gtaaatcttc
    12541 tgatcgatac ggcgacgcag ttcggtcata tccaccgcct ggactttcat tcccagccag
    12601 gattcaaaga agttgtgatc aacaatagaa ccggcgatac ccatcgaaac gccgcccagc
    12661 gacagatagc ttttaccttt catgctggcg acggccaaac cggcgcgggc aaagcgcagc
    12721 agtttttctt caacatcggc aggaatcgat gtgtcatcgg catcctgaac gtcatgaccg
    12781 taaatggaga atgccgggat gcctttctgg ctgtgagctg ccagagccgc tgccaggtaa
    12841 acagcgccgg gacgttcagt gccgttaaag ccccaaatgg ctttcgggcg ggttggatcc
    12901 atgtcgatgg tttcactgcc atagcaccag caaggcgtta ccgtaatggt gaggcctaca
    12961 ttctgactgc tgaatttttc ttcgcaagca gcggcttcag ccatacccgc gatacaggta
    13021 tcggaaatga cacactcgac ggcagctccg caggcatggc gcagtttctc ggtcagcagt
    13081 gcggccgtag ctttcgccat attcattgtt tgttcttcaa gcgactcacg aacacccatg
    13141 cgacgaccgt caataaccgg gcggatacca attttcggta acgtattaat tttcatcagg
    13201 attcctcatc tcgctttatt ggcgctctgc taaggcggag aaaggaaacc accgaagcag
    13261 tagtattggt tattatttgc taactcgagt tagcttaggt ttgtaggctt tttatgcaca
    13321 acttttttgt tgttgaaatg tgaggggctt cacgagaagt ggttaaaata cgatctctgt
    13381 caaattatta tttagcggga taaaaataaa ttttacgtat aatcatatgg ttattagatt
    13441 ttaatggtta tgcatcacag ttttaactca aatgatttgg ctaatttgta tacctaaaat
    13501 agctttaaaa ggcgatcgct aactcgagtt agcaaaatgc gaagaataga ttgatgcgat
    13561 gaatatttat ttaaaggtta ttcgtatcac agtggagaag gctttagtag cgacgtttat
    13621 gccgtacttc acattttcag tttctgatta ttgaggaaag ctgcaatgtc cgaaacattt
    13681 ttgcaaatga aacatatcac caaacgcttt cctggagtgt tggcgcttaa tgacgtgcaa
    13741 tttaccctac gccggggcga ggttcatgca ttattaggtg aaaatggtgc aggaaaatct
    13801 accttaatga aaattttgtc aggggtttat caacctgatg aaggcgaaat aatttttgaa
    13861 gataaaccag tctccttttc tgatccgtta agtgcgcaaa acgtaggtat tactattatt
    13921 caccaggaat ttaatttatt tcccgagctt acagtagagg aaaatatttt tatcggcagg
    13981 gagttttgta aaaaaaatcg ctggcgtctg gatgaaaaac agcaacgcca ggcgacgatt
    14041 gaaattttgc aaaaattaaa tttggcgatc aaaccagata ctctggttgc cgaccttact
    14101 gtagctcagc agcagatggt agaaattgcc aaggccattt cagttaacgc caggattctc
    14161 attatggatg aacctactgc ggcgttaaca gaaactgaaa ttgaaagctt atttcgcgtc
    14221 acccggttat tgaaagagca ggggaccggc attgtgtata tctcccaccg tctggaagag
    14281 ttggcgctaa ttgccgaccg tgcgactgta atgcgtgatg gtcagtacat cagcacggtg
    14341 gattatgagt gcgtaaaaat cagcgatctg attgccatga tggttggtcg cgatttaggg
    14401 aatatctatc cccgccgcga ggccttgcag cagcggattc cggttctgga ggtaaacgga
    14461 ctgacgcgca aaggcgtcct gaacgatatt aattttacgc tttatcgtgg cgagatcctc
    14521 ggctttgccg gattgatggg cgcggggcgt actgaacttg cgcgtgcgat ttttggtgcg
    14581 gattctattg acagcggaac gctaaaatta aatggtaagg aaaccgttat taaagatatt
    14641 tcggatgcta ttcagcaggg aattagttac ctgacggaag accgcaaaaa agaagggctg
    14701 gcgctgaatt tatctgttga acgaaatatt atgttaggaa actaccctga gtattccgat
    14761 cgttttggga atatcgattc gcgtcgttgc caacaaacca gtgaggaaca ggtaaaagcg
    14821 ttaaggatta aaacgccaaa tcttgaacag gcggcattaa atctaagtgg aggaaaccag
    14881 cagaaaatta taattgcacg ttgggtgtgt aaagatactg atattttaat cttcgatgaa
    14941 cccactcgcg gcattgatgt tggcgccaaa ctggaaatct atgagttaat gaatcgcctg
    15001 gtggcaaaag ggaaatcaat catcatgatc tcctctgaat tgccggaggt gttaggaatg
    15061 tgcgaccgta ttttagtgat gcgcagtggt cgaattaccg gcgaacttag cgcaaaagaa
    15121 gcgacacaag aaaaaattat gcaatacgcg acgttagagg attaaattat gactatctct
    15181 gtaacctctc cagacagtaa taataagaaa ataaagataa ataaagaact tttgatgcgc
    15241 cttgcacctc tttttagctt aattatattg gtgctatttt ttagtttcag ctctcccttt
    15301 ttctttaata ccgaaaatat catgacgatt gctctacaaa cgtcggtaat cggcattatg
    15361 gccattgggg taactttcgt cattattacc gctggcatcg atctttcatt gggctccgtg
    15421 gtggcttttt ctggtgtcgc cgtaggtatt tgcgccacgc tgggcctgcc gctgcctgtg
    15481 tgtattattg ctggtgtttt ggccggtgga ttgtgcggct atgtgaatgg tttgttggtc
    15541 actaaaatga ccattccacc gtttatcgct accttgggcc tgatgatgtc cgtcagaggt
    15601 atcaatatgg tgatgactga tgggcgcgcc atctattttg ccgattaccc gatgttcaaa
    15661 acgctcgctc agggacgctt atttgatgtg ctgccttatc ctgttttcta ccttgtcatt
    15721 gtagcgcttg tcggcgctta catcctgaag aaaaccgtca ttggtcgcta tgtctatgcc
    15781 gtaggcagta atgaagttgc tgctcatctt tctggtatta aagtccagcg cgtgaaaatt
    15841 ttcgtctatg ccttctgtgg attattaacg gggattgcag gtgtcattct ggcatctcgt
    15901 ctcaactccg gacaaccgac ggtaggtgtg ggttatgaac tggaagctat tgccgcggta
    15961 gtgattggcg gaaccagctt aatgggtggt attggcacta ttggcggcac aatcattggc
    16021 gctttcatta tgagtgtact gaaaaatggc ctcaatttaa tgggcgtctc ccagttctgg
    16081 caaatggtcg cgatgggcgt cgttgtggtc gctgccgttt acctcgatac attacgcaaa
    16141 aagattcgtt gacttaggct ccctgtttta tcgggaaaca actgccctga cgttaagggt
    16201 gattcactca gaacggagta caacaatgaa aaccaggcat tttatctatg cgttatctct
    16261 gctggcctgc gtaacttcca gcgcactggc gaaggattta aatcttccgg ttgttagtaa
    16321 aggtttccaa catgaattct ggcaaaccgt aaaaatgggg actgaagctg cggctaaaga
    16381 gttgggggat aaaaccagct atgttggccc ggctgatgaa acgcaaatag ccgagcaaat
    16441 tcagttagtc gaaaatgcga tggcgcagaa accgaatggc ttgcttctgg cagccctcga
    16501 cgcgaatgcg ctcgccccac tggtagaaac agcaaattca cgcggtatca aagtggtgac
    16561 ctttgattca ggcatcaatt cagacattcc ggtgagtttt gtcgccacca ataaccgtaa
    16621 agcaggtgct caggcagctg atgccttagc tgcgcaggta aataataaag ggaaagttgg
    16681 gattatcgcg cacgttgcag gaacatcttc cgctattgaa cgctcggaag gcttcatgga
    16741 gcgtatgaaa gaaaaatatc ctgacattaa agtcttaccc gttcagtaca gcgatggtga
    16801 cccgcaaaaa gcgatggata aaactatcga tatgattcag gccaatcccg atcttgctgg
    16861 gatctatggc accaatgaag gttcaacgct cggggtggcg aatgccattg atagccagaa
    16921 tctgaaaggt aaggtgaagg tcattggttt tgatagtacg gaagcgatta ttaacttcct
    16981 gaagaacggc gtcattcagg gctttgttgt tcaggatgca taccagattg gctatcaagg
    17041 gatcaaaacg ttgaacgcgg ccctttcggg gcaagccgtt gaaaaagaga tcgatattcc
    17101 cgtgaaattt gttaatgccg agaatattaa cacgccagaa atcgacaaac tcctgcaccc
    17161 attcggtaag aaataaaaaa ttggggatga attttcatcc ccgttatctc cagcgtcagg
    17221 gaggctttat gcacaacggt tattttctcg gtgtcgatgt cggatcggcc agtgtcaggg
    17281 caggcgttta caatgccagt ggccacagac tggcattcgc tactgcgccc gtctcccagt
    17341 ttcgccctgg cggcgagcgt gtcgaacagt cttcggcgga gatctggcaa caagtttgta
    17401 aaacggtgaa agaggcgaca gctttggcag gcatccccat ttccgcaatt cgctcactgg
    17461 gttttgacgc tacctgctcg ctggttgttc ttgatgaaca aggccagggt ttagcggttt
    17521 cgtccggtgg atctgcgaat cacgacatta ttatgtggat ggatcatcgt gcaactgtcg
    17581 agacggcgca gattaacgca acgaaggatc ccgtattgcg ctacgttggc ggcgaagtga
    17641 gcgtcgagat ggagttacca aagttacgtt ggttgaagac gcatctaccg caaacctggc
    17701 aggccgctca tcgctttttc gatctggcgg attttctggt ctggaaagca acagggcgag
    17761 acgtcgcggg attgtgcacg ctaacgtgta aatggaatta tctggctcat gaacaacgct
    17821 ttagccatag cttactggaa tcggtagacc tgaccgacat gctcgaaaga attcccgccg
    17881 aaattcttcc gcctggcgct gccgtgggaa ctttaactgc caaagctgct gaagaattag
    17941 gattaacgac caatgttatc gtggccagtg gtttgattga tgcccatgcc ggtggcatgg
    18001 cgctggctgg cgcgcaccca tcaggaacgc tggcattgat aagcggaacg tcgaattgcc
    18061 atatgttgtg cagtgaaaaa gaaatattca cccctggcgt ctgggggcca tactggtcgg
    18121 ccatgttgcc aaattactgg ctaactgaag gcgggcaaag cgccgcaggg gcgctggtgg
    18181 agtggacgct tcaggagtcc ggggcaagcg caaatctttt tcacaaggcg cagcaacgcg
    18241 ggtgtcatcc gattcaactg attaatgaat gggtagccgc cctggaagct aacgagtcag
    18301 aacccacacg aaatttacat gtgcttgccg atcatcacgg caaccgttcg ccacgagcgc
    18361 ggcccgatgc cagaggcagt gtttatggct taacactgga gcgtggtgaa aatcagctcg
    18421 cgcggcttta tcttgcgact ttacaggcaa tagcctttgg caccagacat attattgaaa
    18481 ctctcaggga aaatgggcat accattaccc gattaacatt atgtggtggt gcaacgcaca
    18541 accctttatg gctacgcgaa tatgcagacg tgacaggctg cgatatccac ctgatgcaag
    18601 aagaagatgc cgttacattg ggtgcagcaa ttagcggcgc tgttgccagc ggagcatggg
    18661 gggattttac atctgcgtgt aaagcaatgg ttgaagccgg agaggttatt caggtaaatc
    18721 cacaacgccg tgaattttta gagcgtaaat accgggttta cctgacgctt tgggagcaac
    18781 aacaggcggt taaccaactg atgcaataag acaaaagttg aaaatacttt tatgattgtg
    18841 atttgacgtc tggatgttaa ctgggaaata cctatggcta aaacagtaga gcagatagcc
    18901 agcgacctga atttatcggt tacgaccgtg cgactggtgc taaatggaaa agcggaacaa
    18961 tatcgaatca gcgtcaaaac ccaaacgcgt atcaatgaat atgttgaacg ctatggttat
    19021 atcattaatc attccgctcg cagccttaag cttaacaaaa ctgatacatt agggttgatt
    19081 gttcccaaca tatccaacgt cttttttgct acgctggcag aaaaactcga acaacgctgt
    19141 cgacgttccg gatatcaact tacaatcagt tgcacttatg atgatgttga ctatgaaaat
    19201 aaattgacga aagcatttat cgcacgaaat gtagatggtc tttttattgt tccttcgacg
    19261 ctggaaaatc agcagcatca tttacgcaaa attcgtaaac caatggtttt actcgaccgt
    19321 gattttaaat ataccgataa tgcattggta gaaagtaata atatttctgg cggagaaaag
    19381 ttgacacaaa atattcttga ggccgggcag gctccagtat ggtttttgct gggggatgct
    19441 ggtttgccga gtatcagcga tcgtctgaca ggttatttga atgcgttgga aaaaaaaggg
    19501 attacccatc gcgattgggt gcgggaaggt ccggtaaata ctccggaagg cggctatgtc
    19561 attatggaaa aactcattga agagcaggga tgtccgcagg catttatcgc ctcgtcttta
    19621 cctgtgcttg aaggcgctgt ccgcgcgata cgcgaccgtc ttggcgctgt accgccagaa
    19681 atcaatattg gcactttcga tgaacatccc atgctgggat ttctcgccaa caatgtctgg
    19741 tcaatgcagc aggatgaaaa tgcctgggca gaaaaagcgt tcgaaatgat gttaagcgcg
    19801 atcgaagaaa gaccagtcaa aaaaacagtt aaagtagaaa tgaaattaat taagcgcata
    19861 agaaaaaaat aataatcgcc aaaagatatc aggctactga acaatgatat tcagtggcct
    19921 ccccttacta aaaataaccc cgtcttcatt tgcggtgctg ttttacgcaa caaggcgagc
    19981 tgaaactgta tctggagaat agaaatggaa agaacacgtt tatcccgtga aataattgaa
    20041 acctgtctgg aaatgacgcg ccttgggtta aatcagggga cagcggggaa tgtcagcaca
    20101 cgttttgaaa acggcatgtt gattactccc agcggtattc cttatgaaag aatgacggag
    20161 aatatgattg tctatgttga tggcaacggc aaatatgaag aaggtaagat tccttccagc
    20221 gaatggcgtt tccatatggc ggcctatcaa agcagaccgg atgccaacgc ggttgttcac
    20281 aatcatgccg ttcattgcac ggcagtttcc attcttaacc gaccgatccc cgctattcac
    20341 tacatgattg cggcggcggg cggtaattct attccttgcg cgccttatgc gacctttgga
    20401 acacgtgaac tttctgaaca tgttgcgctg gctctcaaaa atcgtaaggc aactttgtta
    20461 caacatcatg ggcttatcgc ttgtgaggtg aatctggaaa aagcgttatg gctggcgcat
    20521 gaagttgaag tgctggcgca actttacctg acgaccctgg cgattacgga cccggtgcca
    20581 gtgctgagcg atgaagagat tgccgtagtg ctggagaaat tcaaaaccta tgggttacgg
    20641 attgaagagt aatttcgtaa agcaacaagg agaaggatga tggctaacag aatgattctg
    20701 aacgaaacgg catggtttgg tcggggtgct gttggggctt taaccgatga ggtgaaacgc
    20761 cgtggttatc agaaggcgct gatcgtcacc gataaaacgc tggtgcaatg cggcgtggtg
    20821 gcgaaagtga ccgataagat ggatgctgca gggctggcat gggcgattta cgacggcgta
    20881 gtgcccaacc caacaattac tgtcgtcaaa gaagggctcg gtgtattcca gaatagcggc
    20941 gcggattacc tgatcgctat tggtggtggt tctccacagg atacttgtaa agcgattggc
    21001 attatcagca acaacccgga gtttgccgat gtgcgtagcc tggaaggact ttccccgacc
    21061 aataaaccca gtgtaccgat tctggcaatc cccaccacag caggcactgc ggcagaagtg
    21121 accattaact acgtgatcac tgacgaagaa aaacggcgca agtttgtttg cgttgatccg
    21181 catgatatcc cgcaggtggc gtttattgac gctgacatga tggatggtat gcctccagcg
    21241 ctgaaagctg cgacgggtgt cgatgcgctc actcatgcta ttgaggggta tattacccgt
    21301 ggcgcgtggg cgctaaccga tgcactgcac attaaagcga ttgaaatcat tgctggggcg
    21361 ctgcgaggat cggttgctgg tgataaggat gccggagaag aaatggcact cgggcagtat
    21421 gttgcgggta tgggcttctc gaatgttggg ttagggttgg tgcatggtat ggcgcatcca
    21481 ctgggcgcgt tttacaacac tccacacggt gttgcgaacg ccatcctgtt accgcatgtc
    21541 atgcgctata acgctgactt taccggtgag aagtaccgcg atatcgcgcg cgttatgggc
    21601 gtgaaagtgg aaggtatgag cctggaagag gcgcgtaatg ccgctgttga agcggtgttt
    21661 gctctcaacc gtgatgtcgg tattccgcca catttgcgtg atgttggtgt acgcaaggaa
    21721 gacattccgg cactggctca ggcggcactg gatgatgttt gtaccggtgg caacccgcgt
    21781 gaagcaacgc ttgaggatat tgtagagctt taccataccg cctggtaaat gcgctgatgt
    21841 gataatgccg gatacgacgt ttgcggcgta tccggctcgc cgttaccgta ccaaccgcaa
    21901 ttgctgtaaa ttgccgtcga tatgcaaatc ggtttgtaag cgggcaatat cgcggcacag
    21961 aaacgccatc tctttatggg tttctaattt tttgcgccac ttttcggcaa ccgcatccag
    22021 attctcatat atcccttcca gactctgaaa ctcgaccagc agctgcgtgg cgctttttgg
    22081 tccgattccc gcaacacccg gtacctttga actgctgatc cccgcaagtc cccagtaatc
    22141 gggcaactgc tgcggttgaa cgccaaattc tttatcgata aatggcgcat ccagccaacg
    22201 tttctggaag taatcacgaa tacgtaatgt cggtgaaagt aactgacagt agcctttatc
    22261 tgtcgaaaca atcgttgcct gatgcccggc ctgtgtcact ttgaccgcca gcgtggcggc
    22321 taagtcatcg gcttcgttgc cgctggttga ccagcacggg acgccgcgtt gctcaaaggc
    22381 ggcgcgtaat gcaggcatct cgtcgtgcaa ctcttccggc attggcggtc gacccgcttt
    22441 gtaatctggt aaacgctgat gacgccagcc gctactgcgg ttttcatcat caaaaacggc
    22501 gaccgcgtgg gttggctggc tgtgcataat gagctgatcg agcgcatgct ggcaggtttc
    22561 gacacagggc gatccctgaa cggcatgaat gcgacgaata agattcagtg catcgacaat
    22621 aagcaaatga acagccacga taaccccctt aaagatcagg cgataagggt aacattcgat
    22681 gccggaacag gctacggctg gatgagaaat cgggaagagg cctcgcaaaa cgaggcctct
    22741 ggagagcgat taatcgcagg caacgatctt cattgccagg ccgccgcgag aggtttcgcg
    22801 gtacttggcg ttcatatctt tacctgtttc gtacatggtt tcgataactt tatcgaggca
    22861 gacgcgcggc tcgctggtac ggcgtagcgc catacgtgcg gcgttcaccg cttttactgc
    22921 cgcaatggcg ttacgctcga tgcatggcac ctggacctgt ccggcgaccg ggtcacacgt
    22981 cagaccgagg ttgtgctcca tggcgatttc cgccgcgatg cacacctgcg ccgggcttgc
    23041 acctaacagt tctgccagac cagccgccgc cattgagcac gccacaccaa cttcaccctg
    23101 gcagccgact tcagcaccag aaattgacgc gttcatctta taaagagaac caatggcact
    23161 ggctaccagc aggtaacgag ccagtgagtt agcgttcact tcgcggataa acttatcgta
    23221 gtacgccagc acagccggga taatcccgca cgcaccgtta gtcggcgcag tcaccacgcg
    23281 accgccagcg gcgttctctt cgttcactgc cagtgcaaac atgttgatcc agtcaacaac
    23341 cgccatcggg tcagtggtgg ttttatcctg gctgaccagc atccggcgta gtgccgcagc
    23401 acggcgtggt acgcgcagtt tgccaggcaa cacgccttcg gtagaaatac cgcgctcaat
    23461 accgccgcgc atgacttccc agacgttcgc caggtgctgt tccagctctt ctttgctgtg
    23521 cagcgccagc tcgtttttca tcatcaggcc agagagtgac agcccggttt cctgacaatg
    23581 tttttgcaga tcggctgctg aactgtacgg ataaggaact tcaaccggtg cgctattctg
    23641 ctggccaaaa tgctcttcat caacgataaa gccaccgcca atagagtagt aagtctggct
    23701 gtaaacgact ttatcgcccg ccagcgcggt aatgcgcata ccgttttcat gcagagaaag
    23761 gttgtctgca tggaagttca tgcactgatc aaccgggaac tccacttcat gctgaccgtt
    23821 tgccagcatc aggcgaccat gagtattcac atcctgaata aaaccaggga tggaatcgat
    23881 atccacggta tccggcaggt tacccgccag gcccataata atggcgatat cagtgtggtg
    23941 gcctttaccg gtcagagaga gcgagccgta cacgtcaacc accacgcggg tcacgtcttt
    24001 aagcagatta cgggcaatca gatcgtcggt aaattgttta cccgctttca ttggtccaac
    24061 ggtatgagaa ctggaagggc caatgccgat tttgaaaata tcgaatacgc taatcatagg
    24121 aaatacatcg cgttaaaacg gaggaagcgc cgcccgaaag cggcgcgaaa ggacttagct
    24181 gaacagagag tagaagattg cggagattgc aatcagaccc atcacgacaa cgaatacgtt
    24241 gctgatgtga ccgctgtact tacgcattgc cggtactttc tgaattgcgt acatcggcat
    24301 caggaacagg atcatcgcga tgattgggcc gcccagggtt tcaatcatac ccaggatgct
    24361 cgggttcagg gtggcaacaa tccaggtcgt taccagcatg aacagcgcag tgatacggtt
    24421 cagcttgttg atttcgatag acttaccttt accacgcaga gatttaatca ccataccgtt
    24481 gaagccttca cgtgcgccca ggtagtggcc gaggaaggat ttggtgatag cgataatcgc
    24541 gataatcgga gccatccacg cgataaccgg tgcgttaaag tggttagcca ggtaagacag
    24601 aatcgagatg ttctgctctt tagccgcagc caggtctgcc ggagtcaggc tcagtacgca
    24661 gctgaagacg aagaacatta cggtcagcac catcatgatg tgtgcgaatg ccaggatctt
    24721 cgagcatttc tgttctgcca tatcgccgta ctcttcacgc ttcgcaacgg cgaaagaaga
    24781 gatgatcgga gagtggttga acgagaacac cattaccgga attgccagcc acagggtcat
    24841 ccacagaccg tttccggttg cagatgcagt gtccagagac agcgtttcca gtgctgcgcc
    24901 gttccactgc gggatcaggt acagagccag cagcatcagt acgccaacaa acgggaatac
    24961 cagaatactc atcgctttaa cgatcatctg ctcaccaaag cgaacgatgg tcatcatacc
    25021 cacgatcagg atcagcgaca gaatcgcacg cggcggtggc gtcataccca gctggtgaga
    25081 catgaagctt tcaactgtat tggtgattgc cacgctataa accagcagga tcgggtagat
    25141 agcgaagaag tagagcaggg taatcagttt acctgcgcca ataccaaagt gttcttctac
    25201 aacctcggtg atgtcttcgc ccgggttttt accagacagt acgaagcgag tcaggccgcg
    25261 gtgagcaaaa aacgtcatcg ggaacgcaag gatagccatg atgatcagcg ggatcatacc
    25321 gccaacaccg gcgttgattg gcaggaacag cacgcccgcg ccgattgccg tgccgtaaag
    25381 gcccagcatc cacatggtgt ctgtcttgcg ccaggcacta cgagagtctt tcgacgcaat
    25441 cgtgctggtt tgagtcgttt ccatctattt ctcctggagg aatgcaaata attcagcttt
    25501 tagccagttc agcccgggaa ggtgcctgac cgtaaaaatg aaatctgtct atcggcgatg
    25561 gcttaatcat ttaccgccgt aataatttga gggcggaaag atacacacaa gttatgtatc
    25621 tatcagtgat cttgatctca aatacaataa tctacttgga agatagatta tcccaacgct
    25681 ttactctgta aatcaaaagt aaaagagaat ttaagggcaa tatttgccca ttaaacgaca
    25741 t
//
LOCUS       DAZFRN010000064        25731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFRN010000064 DAZFRN010000000
VERSION     DAZFRN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.064"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            453..1592
                     /gene="wza"
                     /locus_tag="ACHKUB_002523"
     CDS             453..1592
                     /gene="wza"
                     /locus_tag="ACHKUB_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO6655528.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1598..2041
                     /gene="wzb"
                     /locus_tag="ACHKUB_002524"
     CDS             1598..2041
                     /gene="wzb"
                     /locus_tag="ACHKUB_002524"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO6655529.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRQCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2044..4206
                     /gene="wzc"
                     /locus_tag="ACHKUB_002525"
     CDS             2044..4206
                     /gene="wzc"
                     /locus_tag="ACHKUB_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO6655530.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLTKEGVTLMVEAIHASPGSEFTITKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4299..5138
                     /gene="wcaA"
                     /locus_tag="ACHKUB_002526"
     CDS             4299..5138
                     /gene="wcaA"
                     /locus_tag="ACHKUB_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO6655531.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVSALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5141..5629
                     /gene="wcaB"
                     /locus_tag="ACHKUB_002527"
     CDS             5141..5629
                     /gene="wcaB"
                     /locus_tag="ACHKUB_002527"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO6655532.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5626..6843
                     /gene="wcaC"
                     /locus_tag="ACHKUB_002528"
     CDS             5626..6843
                     /gene="wcaC"
                     /locus_tag="ACHKUB_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO6655533.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFKELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDATLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQHLVREMMALDDKIELHT
                     FGKFSPFTAGNVVNHGFETDKCKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6818..8035
                     /gene="wcaD"
                     /locus_tag="ACHKUB_002529"
     CDS             6818..8035
                     /gene="wcaD"
                     /locus_tag="ACHKUB_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO6655534.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8046..8792
                     /gene="wcaE"
                     /locus_tag="ACHKUB_002530"
     CDS             8046..8792
                     /gene="wcaE"
                     /locus_tag="ACHKUB_002530"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO6655535.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8808..9356
                     /gene="wcaF"
                     /locus_tag="ACHKUB_002531"
     CDS             8808..9356
                     /gene="wcaF"
                     /locus_tag="ACHKUB_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO6655536.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9382..10503
                     /gene="gmd"
                     /locus_tag="ACHKUB_002532"
     CDS             9382..10503
                     /gene="gmd"
                     /locus_tag="ACHKUB_002532"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO6655537.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10506..11471
                     /gene="fcl"
                     /locus_tag="ACHKUB_002533"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10506..11471
                     /gene="fcl"
                     /locus_tag="ACHKUB_002533"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO6655538.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVNDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENRDRFRG"
     gene            11474..11953
                     /gene="gmm"
                     /locus_tag="ACHKUB_002534"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11474..11953
                     /gene="gmm"
                     /locus_tag="ACHKUB_002534"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO6655539.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11950..13173
                     /gene="wcaI"
                     /locus_tag="ACHKUB_002535"
     CDS             11950..13173
                     /gene="wcaI"
                     /locus_tag="ACHKUB_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO6655540.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     CWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRMRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13176..14612
                     /gene="cpsB"
                     /locus_tag="ACHKUB_002536"
     CDS             13176..14612
                     /gene="cpsB"
                     /locus_tag="ACHKUB_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO6655541.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14805..16175
                     /gene="cpsG"
                     /locus_tag="ACHKUB_002537"
                     /gene_synonym="manB"
     CDS             14805..16175
                     /gene="cpsG"
                     /locus_tag="ACHKUB_002537"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO6655542.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVEFIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16230..17624
                     /gene="wcaJ"
                     /locus_tag="ACHKUB_002538"
     CDS             16230..17624
                     /gene="wcaJ"
                     /locus_tag="ACHKUB_002538"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO6655543.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKSGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17626..19104
                     /gene="wzxC"
                     /locus_tag="ACHKUB_002539"
     CDS             17626..19104
                     /gene="wzxC"
                     /locus_tag="ACHKUB_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO6655544.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19476..20756
                     /gene="wcaK"
                     /locus_tag="ACHKUB_002540"
     CDS             19476..20756
                     /gene="wcaK"
                     /locus_tag="ACHKUB_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO6655545.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERYTGMQMVQSVLERIGEVK"
     gene            20753..21973
                     /gene="wcaL"
                     /locus_tag="ACHKUB_002541"
     CDS             20753..21973
                     /gene="wcaL"
                     /locus_tag="ACHKUB_002541"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO6655546.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTEN
                     THTAWTKYNLAARTRWLQDEPTGKLAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21984..23378
                     /gene="wcaM"
                     /locus_tag="ACHKUB_002542"
     CDS             21984..23378
                     /gene="wcaM"
                     /locus_tag="ACHKUB_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO6655547.1"
                     /translation="MPFKKISRRTFLTASSVLAFLHTPFARALPARPSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMHNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKVGIDNATVAIYGCDSFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQMDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGIFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23536..24531
                     /locus_tag="ACHKUB_002543"
     CDS             23536..24531
                     /locus_tag="ACHKUB_002543"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO6655548.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24774..25673
                     /gene="galF"
                     /locus_tag="ACHKUB_002544"
     CDS             24774..25673
                     /gene="galF"
                     /locus_tag="ACHKUB_002544"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO6655549.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRLFIQNLVTRSD"
BASE COUNT         6349 a         6416 c         6951 g         6015 t
ORIGIN      
        1 taaagaaact cctaaaaacc atattgaatg acacttaata taattcttaa aaatagccaa
       61 ttgccgaatt gttaccttgc ctgctattcc gttagctgta acacttcctc ctgcattatt
      121 ggagagccaa tattcaatta ccataatgtc accttatatt cggaagttaa tttccgcaat
      181 aaccctttct catcaacgat tgtatgcgga gagggtttga aaaattaagc taaatcagtg
      241 tattggtagc taaaaagcca ggggcggtag cgtgtctgga tgcctgaaag accagtctga
      301 attattctgt caatagcctg cggatgaaat caattttttt aggactgatg ccagttaaat
      361 ttttattcaa ttaactgcaa ggtaaatgtg catggcgaca ttgctattaa tagtgcacag
      421 gataattact ctgccaaagt gataaataaa caatgatgaa atccaaaatg aaattgatgc
      481 cattattggt gtcagtaacc ttgataagcg gttgcacagt acttccgggc agcaatatgt
      541 cgacgatggg caaagacgtc atcaaacagc aggacgctga tttcgatctc gacaaaatgg
      601 tgaatgttta tccgctgacc ccgcgcctga ttgaccaatt acgcccacgc ccgaatgtag
      661 cgcgccccaa tatgacgctg gaaagcgaga tcgcgaatta ccagtatcgc gtcgggccgg
      721 gcgacgttct gaatgtcacc gtctgggatc acccggaact caccacgcca gccggtcagt
      781 accgcagctc cagcgacacc ggcaactggg tacagcctga cggcactatg ttttacccgt
      841 atatcggcaa ggtccacgta gtcgggaaaa cgctcgctga aatccgcagt gatattaccg
      901 ggcgcttagc gacgtacatc gctgacccgc aggtggatgt taatatcgcc gccttccgct
      961 cgcaaaaggc ctatatctca ggtcaggtga ataaatccgg tcaacaggcg atcaccaacg
     1021 taccactgac cattctcgac gccatcaacg ccgcaggtgg cctgaccgac accgctgact
     1081 ggcgcaacgt ggtgctaaca cacaatggtc gtgaagaacg catttctttg caggcgctga
     1141 tgcaaaacgg cgacctcaat cagaaccgac tgctttaccc cggcgatatt ctctacgtgc
     1201 cgcgtaatga tgatctgaaa gtatttgtga tgggtgaagt taaaaaacag agcaccctga
     1261 aaatggactt tagcggcatg accctgactg aagccctggg caatgctgaa ggcatcgaca
     1321 tgaccacctc caacgccagc ggcatctttg tcattcgtcc gctgaaaggc gagggcgggc
     1381 gtaatggcaa gattgccaat atctaccagc tggatatgtc cgatgccacg tcgctggtga
     1441 tggcgacaga attccgcctg caaccttatg acgtggtgta tgtcaccacc gccccggttt
     1501 cccgctggaa ccgtctgatc aatcagttgc tgccaactat tagcggtgtc cgttacatga
     1561 cggatacagc cagcgacatt cataactggt aatcgtcatg tttaacaaca tcttagttgt
     1621 ctgtgtcggc aatatttgcc gctccccgac ggcggaacgc ttactgcaac gttatcaccc
     1681 ggagctgaaa gtggagtccg ctggactcgg cgcgctggtc ggtaagggcg ctgaccctac
     1741 tgctatcagc gtcgccgcag aacatcaact gtctctggaa ggccactgtg cacggcaaat
     1801 cagccgccgc cagtgtcgca actacgacct gattttgacc atggaaaagc gccatatcga
     1861 acgcttatgc gagatggcac ccgagatgcg cggcaaagtg atgctgtttg gtcactggga
     1921 taacgaatgt gaaatccccg acccgtatcg caaaagccgg gaaacgtttg cagcggtgta
     1981 cacattactt gaacggtctg cccgccagtg ggcgcaggca ttgaacgcag agcaggtata
     2041 agaatgacag aaaaagtaaa acaacatgcc gctccggtaa cgggcagtga tgaaatcgat
     2101 attggtcgcc tggtcggcac cgtcattgaa gcgcgctggt gggtgattgg catcaccgcc
     2161 gtattcgccc tctgtgccgt ggtttacacc ttcttcgcta cgccgatata cagcgccgac
     2221 gcactggtac aaatcgaaca aagcagcggc aattcgctgg tgcaggacat tggctctgca
     2281 ttagccaata aaccgccagc atcagacgcc gagatccagt tgattcgttc acgtctggtg
     2341 cttggtaaaa cggtggacga tctcgacctc gatattgcag tgagcaaaaa cacgttcccg
     2401 attttcgggg cgggctggga tcgcctgatg gggcgtcaga acgagacggt gaaagtgact
     2461 acctttaacc gcccgaaaga gatggcggat caggtcttta cgcttaatgt gctggatgac
     2521 aaaaactaca ccctgagcag tgatggcggc tttagcgccc gtgggcaagc gggccagatg
     2581 ctgacaaaag aaggcgtcac gctgatggtt gaagccattc acgccagccc gggcagtgag
     2641 tttaccatca ccaaatactc cacgctgggg atgatcaacc aactgcaaaa cagcctgacg
     2701 gtaacagaga acggcaaaga cgcaggcgta ctgagcctga cttataccgg tgaagatcgc
     2761 gaacagatcc gcgacattct taacagcatc gcccgtaact atcaggaaca aaatattgag
     2821 cgcaaatcgg cggaagcgtc gaaaagcctc gctttcctcg cgcaacagtt accggaagta
     2881 cgtagccgcc ttgatgttgc cgaaaacaaa ctgaatgcct tccgtcagga taaagattct
     2941 gttgatctgc cgctggaagc gaaagcggtg ctcgattcga tggtgaacat cgacgcccag
     3001 ttgaacgaac tgacctttaa agaggcggaa atctccaagc tgtacaccaa agttcacccg
     3061 gcgtatcgca cgctgctgga gaaacggcaa gcgctggaag acgaaaaagc caaacttaac
     3121 ggtcgcgtaa cggcgatgcc gaaaacccag caggaaattg tccgtctgac ccgcgatgtc
     3181 gagtctggtc agcaggtcta tatgcaactg ctgaataaag agcaggagct gaaaatcacc
     3241 gaagccagca ccgtcggcga tgtgcgcatt gttgacccgg caatcactca gcctggtgtg
     3301 ctaaaaccga agaaagggct gattatcctt ggggcgatta ttcttggcct gatgctctct
     3361 atcgtgggtg tgctgctgcg ctcgttgttt aatcgtggca tcgaaagccc gcaggtgctg
     3421 gaagaacacg gtatcagcgt ctatgccagc atcccgttgt cggaatggca gaaagcgcgc
     3481 gatagcgtca aaaccatcaa agggattaaa cgctataaac agagccagct actggcggtg
     3541 gggaatccaa ccgatctggc gattgaagcc atccgcagtc tgcgtaccag tttgcacttc
     3601 gcgatgatgc aggcgcagaa caatgtgttg atgatgaccg gggttagccc gtcaatcggt
     3661 aaaacctttg tctgcgccaa cctggcggcg gtgatcagcc agaccaataa acgcgtgttg
     3721 ttgatcgact gcgatatgcg caaaggctac acccacgagc tgttgggcac caataacgtc
     3781 aatggcctgt cggaaattct gattggtcag ggcgatatta ctaccgcagc taaaccgacc
     3841 tctattgcca aatttgacct gatcccgcgc ggtcaggtgc cgccaaatcc ttctgaactg
     3901 ttgatgagcg aacgctttgc cgaactggtg aactgggcga gtaaaaacta tgacctggtg
     3961 ttgattgata cgccgccgat tctggcagtg accgatgcgg caattgttgg tcgtcatgtc
     4021 ggaaccacgt taatggtggc gcgttatgcg gtcaacacct tgaaagaagt ggaaaccagt
     4081 ctgagccgct ttgagcaaaa cggtattccg gtgaaagggg tgattctgaa ctccatcttc
     4141 cgccgcgcca gcgcgtatca ggattatggc tattacgaat acgaatataa gtcggatgcg
     4201 aaataacgac gcctgcattc gcaccgcccc gtaggccgga taaggcgctc acgccgcatc
     4261 cggcaagcaa accagctcat aagccgggag aacaacctat gaaagacaat ccgctgattt
     4321 caatctatat gccgacctgg aaccgccaac aactggcgat tcgggcgata aaatcggtcc
     4381 tgcgccagga ctacagcaac tgggagatga tcatcgtgga tgattgctcc acctcctggg
     4441 aacaactgca acagtacgtc agcgccctca acgatccgcg tatcacttac atccacaacg
     4501 acattaacag cggggcgtgc gcggtacgta accaggcgat tatgctggcg caaggggaat
     4561 acatcaccgg gatcgatgat gatgatgaat ggacacccaa ccgcctgagc gtcttcctcg
     4621 cccataaaca gcaactggtc actcacgcct ttttgtacgc taacgactac gtttgccagg
     4681 gcgaagtcta ttcccaacca gcaagtttgc cgctgtatcc caaatcgccg tattcacgcc
     4741 gcctgttcta taaacgcaat atcattggca atcaggtctt tacctgggcg tggcgtttca
     4801 aagagtgttt gttcgatacc gaactgaaag cggcacagga ttacgacatc ttcctgcgga
     4861 tggtggtgga gtatggcgaa ccgtggaaag tggaagaggc gacgcagatc ctgcatatca
     4921 atcacggtga aatgcagatc acctcgtcac cgaaaaaatt ctccggatac ttccactttt
     4981 accgcaagca caaagataaa tttgaccgcg ccagcaaaaa atatcagctg tttactctct
     5041 accagatccg caataaacgt atgacctggc gcactttgct gacgctgctg tcggtgcgta
     5101 acggcaaacg tctggctgat ggtattcggg ggcgctaaag atgctggaag atctgcgcgc
     5161 caacagctgg agtttacgcc catgctgcat ggttcttgcc tatcgcgttg ctcatttttg
     5221 ttcggtgtgg cgcaaaaaga acgttctcaa caatctgtgg gcggccccgc tgctggtgct
     5281 gtatcgcatt atcaccgaat gctttttcgg ttatgaaatc caggcagccg caaccattgg
     5341 ccgccgcttt actatccatc acggttacgc cgtagtgatc aataaaaacg tggtagcggg
     5401 ggatgatttc accattcgcc acggcgtcac tatcggcaat cgtggtgccg ataacatggc
     5461 atgtccacac attggcaacg gcgtcgaact cggtgccaac gtcattattc ttggtgatat
     5521 cacgcttggt aacaacgtca ccgtgggtgc gggcagcgtg gtgctcgact ctgtcccgga
     5581 caacgcgctg gtggtgggag aaaaagcgcg agtgaaggta attaaatgaa tattctgcaa
     5641 tttaatgtgc gactggcgga aggcggggcg gcaggtgtgg cgttagatct ccaccagcgt
     5701 gcgctgcaac aggggctggc gtcacatttt gtctacggct acggcaaagg cggcaaagag
     5761 agcgtcagcc atcagaacta tccgcaggta atcaaacata cgccgcggat gaccgcgatg
     5821 gcgaacattg ccctgtttcg tctgtttaat cgcgatctgt ttggcaattt caaggagtta
     5881 tatcgcacca ttactcgcac accgggtccg gtggtcctgc attttcatgt gctgcacagc
     5941 tactggttga atcttaagag cgtggtgcgc ttttgcgaaa aggtgaaaaa ccataaaccg
     6001 gacgccactc tggtctggac gctgcacgat cactggagcg ttaccgggcg ctgcgccttt
     6061 accgacggtt gtgaaggctg gaaaacgggc tgccagaaat gcccgacctt aaataattat
     6121 ccgccggtga agattgatcg cgcacaccaa ctggtggcgg gcaaacgcca gttattccgt
     6181 gagatgctgg cgctgggctg tcagtttatt tcccccagcc agcatgtggc tgacgctttc
     6241 aatagcctgt acggtccagg gcgttgccgg attatcaata acggtattga tatggcaacc
     6301 gaagcgattc tggcggactt gcctccggtg cgcgaaaccc agggcaagcc gaaaatcgcg
     6361 gtggtggcgc atgacctgcg ttacgacggc aaaactaacc agcacctggt gcgcgagatg
     6421 atggcgctgg acgacaaaat cgaactgcat acctttggta agttctcgcc gttcaccgct
     6481 ggcaacgtgg ttaatcacgg ctttgaaact gacaagtgca agttgatgag cgcgctcaat
     6541 cagatggatg cgctggtgtt cagttctcgc gtcgataact acccgctgat tttgtgtgag
     6601 gcgctatcga ttggtgtgcc ggtgattgcc acccatagcg acgcggcgcg ggaagtgctg
     6661 caaaaatccg gcggtaaaac cgtcagcgaa gaagacgtgc tgcaactggt gcagttaagc
     6721 aaaccggaaa tcgcgcaggc gatatttggt accacgctgg ctgagttcag ccaacgcagc
     6781 cgcgccgcct acagtggaca acagatgctg gaggagtatg tcaacttcta tcagaatctg
     6841 tagctacctg ctgctgccgc tgatctacct gctggttaac gtcaaaatcg cccagcttgg
     6901 cgaaagtttc cccatcacca tcgtcacttt tttacctgtc ttgttactgc tgtttttaga
     6961 acgcatcagc gttaaaaaat tgatgattgc cttagggatt ggtgcgggac tcacggcgtt
     7021 taactacctg tttggtcagt cgttggatgc cggtaaatac gtcacgtcaa ccatgctgtt
     7081 tgtctatatt gtgatcatta ttggcatggt gtggagtatt cgttttaaaa caatttcgcc
     7141 acacaaccat cgcaagatat tacgtttctt ttatctggtg gtcgggctgg tggtggcgct
     7201 ggcggcagtg gagatggcgc aaattatcct caccggcggc agcagtatta tggagtcgat
     7261 ttcgaaatat ctgatttaca gcaacagcta tgtgctgaat ttcattaaat tcggcggcaa
     7321 gcgcacgaca gcactttatt tcgaaccggc atttttcgct ctggcattaa tctcaatttg
     7381 gctcagcatc aaacagtttg gtatcaaaac gcctaaaacc gatgctatga ttctcgcagg
     7441 gataatatta tccggatcgt tttcaggggt tatgaccttt atcctgtttt atttgctgga
     7501 gtgggcattt caatatctga ataaagaggc gattaagaaa aagttaccat tagcattgat
     7561 ttctctggct gtattcctgg ttggtgtggt aatcgcgttt ccttatattt ccacccgtct
     7621 gggcgattta ggtacggaag gatcgtcatc gtattatcgt attgtcggtc cgctggtgat
     7681 ggtcggttat tctttgaccc atattgacgg tgtagtcaga tttggctcac tttatgaata
     7741 tgtcgcatca ttcggaatat ttaacggtgc ggatgtcgga aaaaccatag acaatggttt
     7801 gtatctgctg attatttatt tttcctggtt cgcggtgttt ttatcgctgt ggtacatggg
     7861 gaaagtgata aaaatgatga tcaacgcttt tggtgataac cgcaattttc gcgtgcaatt
     7921 atatcttttt actccggtat cgctgttttt taccggttcg atatttagcc cggaatatgc
     7981 atttttaatc gtctgtccgt ttattttgcg aaaagcgtta aatattacga ggtaagaata
     8041 agaacatgtt gcttagcata atcactgtcg cgtttcgtaa cctcgaaggg atagtcaaaa
     8101 cacatgcctc gctggcgcat ctggcgcagg cggaagatat cagcttcgaa tggattgttg
     8161 tcgatggcgg ttccaatgac ggcacccgtg agtatctgga aaatctcaat ggtatctata
     8221 acctacgctt tgtcagcgag ccagataacg gtatctatga cgccatgaac aaaggcatta
     8281 cgatggcaca aggcaagttc gcgttgtttc tcaattcggg cgatattttt catcagaatg
     8341 ccgcaaattt tgtccgtaag ttaaaaatgc aaaaagataa cgtgatgatc accggcgatg
     8401 cgctgctgga ttttggcgac gggcataaaa ttaaacgtag cgccaaaccg ggctggtata
     8461 tttatcacag cctgcccgcc agtcatcagg cgatattttt cccggtatcc ggcttgaaga
     8521 aatggcgtta tgacctggaa tataaagttt cttccgacta cgcgctggca gccaaaatgt
     8581 ataaagccgg ttatgcattt aaaaaactca atggcctggt gtctgaattt tccatgggtg
     8641 gggtatctac caccaataat atggaattgt gtgctgacgc gaaaaaagtc caacgacaaa
     8701 tattacatgt acctggcttt tgggctgaat tatcctggca tttacgccaa cgtactacct
     8761 caaagacgaa agccttatat aacaaagtct gaatataagg gaaaacgatg caggatttaa
     8821 gtggattctc ggtgccgaaa gggttccggg gcggcaacgc tattaaagtg caattatggt
     8881 gggcagtaca ggcaacaata tttgcctggt cgccacaagt attgtatcgc tggcgggctt
     8941 ttttattacg tttattcgga gcaaaaatag gaaaaaacgt agttattcgt ccgtcagtaa
     9001 aaattaccta tccgtggaaa ttaaccttag gtgattacgc gtgggtcggc gatgacgtca
     9061 atttatatac cctcggtgaa ataaccattg gcgcacattc ggtgatatcg caaaaaagtt
     9121 atttatgcac cggtagccac gaccatgcaa gtcaacattt caccattaac gccacgccta
     9181 ttgtgattgg cgagaaatgc tggctggcaa ccgatgtctt tgttgcccca ggcgtcacaa
     9241 tcggcgacgg caccgtcgtg ggtgcacgaa gcagtgtttt taaatcgctt ccggcaaatg
     9301 tggtttgccg ggggaatccc gcagtggtga tacgcgaacg cgttgaaact gaataaattc
     9361 aaaaatacag aggaataaga catgtcaaaa gtcgctctca tcaccggtgt aaccggacaa
     9421 gacggttctt acctggcaga gtttctgctg gaaaaaggtt acgaggtgca tggtattaag
     9481 cgtcgtgcat cgtcattcaa caccgagcgc gtggatcaca tttatcagga tccgcacacc
     9541 tgcaacccga aattccatct gcattatggc gacctgagtg atacctccaa cctgacgcgc
     9601 attttgcgtg aagtacagcc ggatgaagtg tacaacctgg gggcaatgag ccacgttgcg
     9661 gtttcttttg agtcaccgga atataccgct gacgtcgacg cgatgggtac gctgcgcctg
     9721 ctcgaggcga tccgcttcct cggtctggaa aagaaaactc gtttctatca ggcttccact
     9781 tctgaactgt acggtctggt gcaggaaatt ccgcagaaag aaaccacgcc gttctatccg
     9841 cgatctccgt atgcggtcgc caaactgtat gcctactgga tcaccgttaa ctaccgcgaa
     9901 tcctacggca tgtacgcctg taacggtatt ctcttcaacc atgaatcccc gcgccgcggc
     9961 gaaaccttcg ttacccgcaa aatcacccgc gcaatcgcca acatcgccca ggggctggag
    10021 tcgtgcctgt acctcggcaa tatggattcc ctgcgtgact ggggccatgc caaagactac
    10081 gtaaaaatgc agtggatgat gctgcaacag gaacagccag aagatttcgt tatcgcgacc
    10141 ggcgttcagt actccgtacg tcagttcgtg gaaatggcgg cagcacagct gggcatcaaa
    10201 ctgcgctttg aaggcacggg tgttgaagag aagggcattg tggtttccgt caccgggcat
    10261 gacgcgccgg gcgttaaacc gggtgatgtg attatcgccg ttgacccgcg ttacttccgt
    10321 ccggctgaag ttgaaacgct gctcggcgac ccgaccaaag cgcacgaaaa actgggctgg
    10381 aaaccagaaa tcaccctcag agagatggtg tctgaaatgg tggctaatga cctcgaagcg
    10441 gcgaaaaaac actctctgct gaaatctcac ggctacgacg tggcgatcgc gctggagtca
    10501 taagcatgag tagacaacgc atttttattg ctggccatcg cgggatggtc ggttctgcca
    10561 tcaggcggca gctcgaacag cgcggtgatg tggaactggt attacgcacc cgcgacgagc
    10621 tgaacctgct ggacagccgc gccgtgcatg atttctttgc cagcgaacgt attgaccagg
    10681 tctatctggc ggcggcgaaa gtgggcggca ttgttgccaa caacacctat ccggcggatt
    10741 tcatctacca gaacatgatg attgagagca acatcattca cgccgcgcat cagaacgacg
    10801 tgaacaaact gctgtttctt ggatcgtcct gcatctaccc gaaactggca aaacagccga
    10861 tggcagaaag cgagttgttg cagggcacgc tggagccgac caacgagcct tacgccattg
    10921 ccaaaatcgc cgggatcaaa ctgtgcgaat catacaaccg ccagtacgga cgcgattacc
    10981 gctcagtcat gccgaccaac ctgtacgggc cacacgataa cttccacccg agtaattcgc
    11041 atgtgatccc agcattgctg cgtcgcttcc acgaggcgac ggcacagaat gcgccggacg
    11101 tggtggtgtg gggcagtggt acaccgatgc gtgaatttct gcacgtcaat gatatggcgg
    11161 cggcgagcat tcatgtcatg gagctggcgc acgaagtctg gctggaaaac acccagccga
    11221 tgctgtcaca cattaacgtc ggcacgggcg ttgactgcac tatccgcgag ctggcgcaaa
    11281 ccatcgccaa agtggtgggt tacaaaggtc gggtggtttt tgatgccagc aagccggatg
    11341 gtacgccgcg caaactgctg gatgtgacgc gcctgcatca gcttggctgg tatcacgaaa
    11401 tctcactgga agcggggctt gccagcactt accagtggtt ccttgagaat cgagaccgct
    11461 ttcgggggta atgatgtttt tacgtcagga agactttgcc acggtagtgc gctccacacc
    11521 gcttgtctct ctcgacttta ttgtcgagaa cagtcgcggc gagtttctgc ttggcaaaag
    11581 aaccaaccgc ccggcgcagg gttactggtt tgtgccggga gggcgcgtgc agaaagacga
    11641 aacgctggaa gccgcatttg agcggctgac gatggcggaa ctggggctgc gtctgccgat
    11701 aacagcaggc cagttttacg gtgtctggca gcacttttat gacgataact tctccggcac
    11761 ggatttcacc actcactatg tggtgctcgg ttttcgcttc agagtcgctg aagaagatct
    11821 gttactgccg gatgagcagc atgacgatta ccgctggctg acgccggacg cactgctcgc
    11881 cagtgacaat gttcacgcta acagccgcgc ctattttctc gctgagaagc gtgccggagt
    11941 acccggatta tgaaaatact ggtctacggc attaactact cgccggagtt aaccggcatc
    12001 ggcaaataca ccggcgagat ggtggaatgg ctggcggcgc aaggtcatga ggtgcgggtt
    12061 attaccgcac cgccttacta cccgcagtgg caagtgggcg agaactattc cgcctggcgg
    12121 tacaaacgag aagagggggc cgctacggtg tggcgctgcc cgctgtacgt gccaaaacag
    12181 ccgagcaccc tgaaacgcct gttgcatctc ggcagttttg ccgtcagcag ttttttcccg
    12241 ttgatggcgc aacgttgctg gaagccggat cgcattatcg gcgtagtgcc aacgctgttt
    12301 tgtacgccgg gaatgcgcct gctggcgaaa ctctctggtg cgcgtaccgt gctgcatatt
    12361 caggattacg aagtggacgc catgctgggg ctgggccttg ccggaaaagg caaaggcggc
    12421 aaagtggcac agctggcgac ggcgttcgaa cgtagcggac tgcataacgt cgataacgtc
    12481 tccacgattt cgcgttcgat gatgaataaa gccatcgaaa aaggcgtggc ggcggaaaac
    12541 gtcatcttct tccccaactg gtcggagatt gcccgttttc agcatgttgc agacgccgat
    12601 gttgatgccc ttcgtaacca gcttggcctg ccggataaca aaaaaatcat tctttactcc
    12661 ggcaatattg gtgaaaagca ggggctggaa aacgttattg aagctgccga tcgcatgcgc
    12721 gatgaaccgc tgatttttgc cattgtcggg cagggcggcg gcaaagcgcg gctggaaaaa
    12781 atggcgcaac agcgtggtct gcgcaacatg caattttttc cgctgcaatc gtatgacgct
    12841 ttacccgcac tgctgaagat gggcgattgc catctggtgg tgcaaaaacg cggcgcggca
    12901 gatgccgtat tgccgtcgaa actgaccaat attctggcgg taggcggtaa cgcggtgatt
    12961 accgcagagg cccacacaga actgggacag ctttgcgaaa cctttccggg cattgcggtt
    13021 tgcgtagaac cggaatcggt cgaggcgctg gtggcgggga ttcgtcaggc gctcctgctg
    13081 cccaaacaca acacggtggc acgtgaatat gccgaacgca cgctcgataa agagaacgtg
    13141 ttacgtcaat ttataaatga tattcgggga taattatggc gcagtcgaaa ctctatccag
    13201 ttgtgatggc aggtggctcc ggtagccgct tatggccgct ttcccgcgta ctttacccca
    13261 agcagttttt atgcctgaaa ggcgatctca ccatgctgca aaccaccatc tgccgcctga
    13321 acggcgtgga gtgcgaaagc ccggtggtga tttgcaatga gcagcaccgc tttattgtcg
    13381 cggaacagct gcgtcaactg aacaaactca ccgagaacat tattctcgaa ccggcagggc
    13441 gtaacactgc acctgccatt gcactggcgg cgctggcggc aaaacgtcat agcccggaga
    13501 gcgacccgtt aatgctggtg ctggcggcgg atcatgtgat tgccgatgaa gacgcttttc
    13561 gcaccgccgt gcgtaatgcc atgccgtatg ccgaagcggg caagctggtg accttcggca
    13621 ttgtgccgga tctacctgaa accggatatg gctatattcg tcgcggtgaa gtgtcggcgg
    13681 gtgagcagga tacggtggcc tttgaagtgg cgcagtttgt cgaaaaaccg aatctggaaa
    13741 ccgctcaggc ctatgtggca agcggcgaat attactggaa cagcggtatg ttcctgttcc
    13801 gcgccggacg ctatctcgaa gaactgaaaa aatatcgccc ggatattctc gatgcctgtg
    13861 aaaaagcgat gagcgccgtc gatccggatc tcgattttat tcgcgtggat gaagaagcgt
    13921 ttctcgcctg cccggaagag tcggtggatt acgcggtcat ggaacgtacg gcagatgctg
    13981 ttgtggtgcc gatggatgcg ggctggagcg atgttggttc ctggtcttca ttatgggaaa
    14041 tcagcgccca caccgccgag ggcaacgttt gtcacggtga tgtgattaat cacaaaacag
    14101 aaaacagcta tgtgtatgcc gaatctggcc tcgtaaccac cgtcggggtg aaagatctgg
    14161 tggtagtgca gaccaaagat gcggtgctga ttgccgaccg taacgcggtg caggatgtga
    14221 aaaaagtggt cgagcagatc aaagccgacg gtcgccatga gcatcgggtg catcgcgaag
    14281 tgtatcgtcc gtggggcaaa tatgactcta tcgacgcggg cgaccgctac caggtgaaac
    14341 gcatcaccgt gaaaccgggc gaaggcttgt cggtacagat gcaccatcac cgcgcggaac
    14401 actgggtggt ggtcgcggga acggcaaaag tcaccattga cggtgatatc aaactgcttg
    14461 gtgaaaacga gtccatttat attccgctgg gggcgacgca ctgcctggaa aacccgggga
    14521 aaattccgct cgatttaatt gaagtgcgct ccggctctta tctcgaagag gatgatgtgg
    14581 tgcgcttcgc ggatcgctac ggacgggtgt aaacgtcgca tcaggcgctg tttgtcggat
    14641 gcggcgtgaa cgccttatcc gacctacggt tcggatttgt aggcctgata agacgcggca
    14701 gcgtcgcatc aggcaatgaa tgcgaaaccg cggtgtaaat aacgacaaaa acaaaattgg
    14761 ccgcttcggt cagggccaac tattgcctga aaaagggtaa cgacatgaaa aaattaacct
    14821 gctttaaagc ctatgatatt cgcgggaaat taggcgaaga actgaatgaa gatattgcct
    14881 ggcgcattgg tcgcgcctat ggcgaatttc tcaaaccgaa aaccattgtg ttaggcggtg
    14941 atgtccgcct caccagcgaa accttaaaac tggcgctggc gaaaggttta caggatgcgg
    15001 gcgtcgatgt gctggatatt ggcatgtccg gcaccgaaga gatctatttc gccacgttcc
    15061 atctcggcgt ggatggcggc atcgaagtta ccgccagcca taatccgatg gattacaatg
    15121 gcatgaagct ggtgcgcgaa ggggctcgcc cgatcagcgg tgataccgga ctgcgcgacg
    15181 tccagcgcct ggcagaagcc aacgactttc ctcccgtcga tgaaaccaaa cgcggtcgct
    15241 atcagcaaat caacctgcgt gacgcttacg ttgatcacct gttcggttat atcaatgtca
    15301 aaaatctcac gccgctcaag ctggtgatta actccgggaa cggcgcggcg ggtccggtgg
    15361 tggacgccat cgaagcccgc tttaaagccc tcggcgcgcc cgtggaattc atcaaagtgc
    15421 acaacacacc ggacggcaat ttccccaacg gtattcctaa cccgctactg ccagaatgcc
    15481 gcgacgacac ccgcaatgcg gtcatcaaac acggcgcgga tatgggcatt gcctttgacg
    15541 gtgattttga tcgctgcttc ctgtttgacg aaaaagggca gtttatcgaa ggctactaca
    15601 ttgtcggcct gctggcagaa gcgttcctcg aaaaaaatcc cggcgcgaag atcatccacg
    15661 atccacgtct ctcctggaac accgttgatg tggtgactgc cgcaggcggc accccggtaa
    15721 tgtcgaaaac cggacacgcc tttattaaag aacgtatgcg caaggaagac gccatctacg
    15781 gtggcgaaat gagcgcccac cattacttcc gtgatttcgc ttactgcgac agcggcatga
    15841 tcccgtggtt gctggtagcc gaactggtat gcctgaaaga gaaaacgctg ggcgaactgg
    15901 tacgcgaccg gatggctgcg tttccggcaa gcggtgagat caacagcaaa ctggcgcaac
    15961 ccgttgaggc gattaatcgc gtcgaacagc attttagccg tgaggcgctg gcggtggatc
    16021 gcaccgatgg catcagcatg acctttgccg actggcgctt taacctgcgc tcctccaaca
    16081 ccgaaccggt ggtgcggttg aatgttgaat cgcgcggtga tgtgccgctg atggaagcgc
    16141 gaacgcgaac tctgctgacg ttgctgaacg agtaatgtcg gatcttccct taccccactg
    16201 cgggtaaggg gctaataaca ggaacaacga tgacaaatct aaaaaagcgc gagcgagcga
    16261 aaaccaatgc atcgttaatc tctatggtgc aacgcttttc agatatcacc atcatgtttg
    16321 ccggactatg gctggtttgc gaagtcagcg gactgtcatt cctctacatg cacctgttgg
    16381 tggcgctgat tacgctggtg gtattccaga tgctgggtgg catcaccgat ttttatcgct
    16441 catggcgcgg tgttcgggca gcgacagaat ttgccctgtt gctacaaaac tggacgttaa
    16501 gcgtgatttt cagcgccgga ctggtggcgt tcaacaatga tttcgacacg caactaaaaa
    16561 tctggctggc gtggtatggg ctgaccagta tcggactggt ggtttgccgt tcatgtattc
    16621 gtattggggc gggctggctg cgaaatcatg gctataacaa gcgcatggtc gcggtggcgg
    16681 gggatttagc cgccggacaa atgttgatgg agagcttccg taatcagccg tggttagggt
    16741 ttgaagtggt gggcgtatac cacgacccaa aaccgggcgg cgtttctaac gactgggcgg
    16801 gtaatctgca acagctggtc gaggacgcga aatcgggcaa gattcataac gtctatatcg
    16861 cgatgcaaat gtgcgacggc gcgcgagtga aaaaactagt ccatcaactg gcggacacca
    16921 cctgttcggt gctgctgatc cccgatgtct ttaccttcaa cattctccat tcacgtcttg
    16981 aagagatgaa cggcgttccg gtggtgccgc tttacgacac gccgctttcc ggggttaacc
    17041 gcctgctcaa acgtgcggaa gacattgtgc tggcgacgct tatcctgctg ctgatctccc
    17101 cggtgctgtg ctgcattgcg ctggcggtga aactcagttc acctgggccg gttattttcc
    17161 gccagactcg ctacggcatg gatggcaagc cgatcaaagt gtggaagttc cgttccatga
    17221 aagtgatgga gaacgacaaa gtggtgaccc aggcgacgca gaacgatccg cgcgtcacca
    17281 aagtggggaa ctttctgcgc cgcacctcgc tggatgaact gccgcagttt atcaatgtgc
    17341 tgaccggggg gatgtcgatt gtcggtccac gtccgcacgc ggtagcgcat aacgaacagt
    17401 atcgccagct cattgaaggc tacatgctgc gccataaggt gaaaccgggc attaccggct
    17461 gggcgcagat taacggctgg cgcggcgaaa ccgacacact ggagaaaatg gaaaaacgcg
    17521 tcgagttcga ccttgagtac atccgcgaat ggagcgtctg gttcgatatc aaaatcgttt
    17581 tcctgacggt gttcaaaggt ttcgttaaca aagcggcata ttgatatgag cttacgtgaa
    17641 aaaaccatca gcggcgcgaa gtggtcggcg attgccacgg tgatcatcat cggcctcggg
    17701 ctggtgcaga tgaccgtgct ggcgcggatt atcgacaacc accagttcgg cctgctgact
    17761 gtgtcgctgg tgattatcgc gctggcagat acgctttctg actttggtat cgctaactcg
    17821 attattcagc gaaaagaaat cagccacctt gaactcacca cgctgtactg gctgaacgtc
    17881 gggctggggc ttgtggtgtg cgtggcggtg tttttgttga gtgatgccat tggcgacgtg
    17941 ctgaataacc cggacctggc accgttgatt aaaacattgt cgctggcgtt tgtggtgatc
    18001 ccccacgggc aacagttccg cgcgttgatg cagaaggagc tggagttcaa caaaatcggc
    18061 atgatcgaaa ccagcgcggt gctggcgggc ttcactttta cggtggttag cgcccatttc
    18121 tggccgctgg cgatgaccgc gatcctcggt tatctggtca atagtgccgt gagaacgctg
    18181 ctgtttggct actttggccg caaaatttat cgccccggtc tgcatttctc gctggcgtcg
    18241 gtggcaccga acttacgctt tggtgcctgg ctgacggcgg acagcatcat caactatctc
    18301 aacactaacc tttcaacgct ggtgctggcg cgtattctcg gcgcgggcgt ggcagggggc
    18361 tacaacctgg cgtacaacgt ggccgttgtg ccgccgatga agctgaaccc aatcatcacc
    18421 cgcgtgttgt ttccggcatt tgccaaaatt caggacgaca ccgaaaagct gcgcgtcaac
    18481 ttctacaagc tgctgtcggt ggtggggatt atcaactttc cggcgctgct ggggctgatg
    18541 gtggtgtcga ataactttgt accgctggtc tttggtgaga agtggaacag cattattccg
    18601 gtgctgcaat tgctgtgtgt ggtgggtctg ctgcgctccg taggtaaccc gattggttcg
    18661 ctgctgatgg cgaaagcgcg ggtcgatatc agctttaaat tcaacgtatt caaaacattt
    18721 ctgtttattc cggcgattgt tattggtggg cagatggcgg gcgcaatcgg cgtcacgctc
    18781 ggtttcctgc tggtgcagat tatcaacacc attctgagtt acttcgtgat gattaaaccg
    18841 gttcttggtt ccagttatcg ccagtacatc ctgagtttgt ggctgccgtt ttacctctcg
    18901 ctgccgacgc tggtggtcag ttatgcgctg ggcattgtgc tgaaagggca actggcgctg
    18961 gggatgctgc tggcggtgca aatagccgca ggcgtgctgg cgtttgtggt gatgattgtg
    19021 ctgtcgcgcc atccgctggt ggtggaagtg aagcgtcagt tttgtcgcag cgaaaaaatg
    19081 aaaatgcttt tacgggcggg gtgaatggtt gttttaggct ccgacaacgg ttgccgggtg
    19141 cagcgtaaaa cgccttatcc atcctacggt tctgtgctcg ggacctgtag gcctgataag
    19201 acgcgtcagc gtcgcatcag gcactgaatg ccggatgcgg cgtgaacgcc ttatccggcc
    19261 tacagttctg tgctcgggtc ttgtaggcct gataagacgc gccagcgtcg catcaggcac
    19321 tgaatgccgg atgcggcgtg aacgccttat ccggcctacg gttctgtgct cgggtcttgt
    19381 aggcctgata agacgcgcca gcgtcgcatc aggcaaaaaa cggcaacgaa cacaacaaac
    19441 acagcctcac ctggcatcaa atttatgagg aaaaaatgaa attacttatt ctgggcaacc
    19501 acacttgcgg caatcgtggc gacagcgcca tcttgcgcgg cttacttgat gccatcaaca
    19561 tcctcaatcc acatgccgaa gtggacgtga tgagccgcta tccggtcagt tcttcctggc
    19621 tgctcaaccg cccggtaatg ggtgatccgc tgttcctgca aatgaaacaa cacaacagcg
    19681 cggcgggcgt tgtcgggcgc gttaaaaaag tcctccgtcg ccgctatcag caccaggtat
    19741 tgctctcacg cgtcaccgac actggcaagc tgcgtaatat cgccatcgcc cagggattca
    19801 ccgacttcgt gcgactgctg tcaggttacg acgccattat ccaggtcggc ggatcgtttt
    19861 ttgtcgatct ctatggcgtg ccgcagtttg aacatgcgct ttgcacgttt atggcgaaaa
    19921 agccgctgtt tatgattggt cacagcgtcg gtcccttcca ggatgagcaa tttaaccaac
    19981 tggcgaacta cgtttttggt cactgcgacg cgctgatcct gcgcgaatcg gtcagccttg
    20041 aactgatgaa acgcagcaat atcaccactg caaaagttga acatggcgtc gataccgcgt
    20101 ggctggtcga tcaccacacc gaagatttca ccgccagcta tgccgtccaa cactggctgg
    20161 acgttgccgc acaacagaaa acggtggcca ttaccctgcg cgaactggca ccgttcgaca
    20221 aacgtctcgg caccactcag caagcgtatg aaaaagcctt tgccggggtg gtcaatcgca
    20281 ttctcgacga aggctatcag gtgattgcgc tttccacctg tacgggcatc gacagctata
    20341 acaaagacga ccgcatggtg gcgctcaacc tgcgccagca catcagcgat cctgcccgtt
    20401 atcacgtagt gatggatgaa cttaacgatc tggaaatggg caaaattctc ggtgcctgtg
    20461 aactcaccgt cggtacgcgc ctacactcag ccattatctc gatgaacttt gccactccgg
    20521 cgattgccat caactacgaa cataaatccg ccgggattat gcagcagctg gggctgccag
    20581 agatggcgat tgatatccgt catttactgg acggcagctt gcaagcgatg gttgcggata
    20641 ccttaggcca acttccggcg ctgaacgcgc gacttaacga agccgtcagt cgtgagcgtt
    20701 atacgggaat gcagatggtg cagtctgtac tggaacgcat cggggaggtg aaatgaaggt
    20761 cggcttcttt ttactgaaat ttccgctgtc gtcggaaacc ttcgtcctca accaaattac
    20821 cgcgtttatt gatatgggct ttgaggtgga gattctcgcg ttgcaaaaag gcgacacaga
    20881 gaacacccac acggcatgga cgaaatacaa ccttgccgcc agaacccgct ggttacagga
    20941 cgaacctacg ggcaaattgg cgaagctgcg ccaccgcgcc agccagacct tacgcggcat
    21001 tcatcgtaaa aatacctggc aggcgctcaa cctcaaacgc tatggtgccg agtcgcggaa
    21061 cctgattttg tctgccattt gcggtcaggt cgcaacacca tttcatgccg atgtctttat
    21121 cgctcatttt ggtcctgcgg gggtaaccgc agcaaaacta cgcgaactgg gtgtcattcg
    21181 cggcaaaatt gccactattt tccacggcat tgatatttcc agtcgggaag tgctcaacca
    21241 ttacactccc gaatatcaac aactgtttcg acgtggcgac ctgatgctac cgataagcga
    21301 tctgtgggcc ggaaggctgc aaaaaatggg ctgcccgagg gaaaaaatcg ccgtatcgcg
    21361 tatgggcgtg gacatgacgc gctttagccc gcgtccggtg aaagcgcccg caacgccgct
    21421 ggaaatcatc tccgtcgcac gcttaaccga aaaaaaaggc ctgcatgtgg cgatcgaagc
    21481 ctgccgtcag ttgaaagagc agggcatggc atttcgctat cgcatcctcg gcattggccc
    21541 gtgggaacga cgcctgcgta ccctcatcga acaatatcaa ctggaagatg tggtagagat
    21601 gccgggcttt aaaccgagcc acgaagtgaa agcgatgctc gacgacgcgg atgtcttcct
    21661 gttgccatcg gtaacgggcg cggatggcga tatggaaggc attccggtag cgctgatgga
    21721 agcgatggcg gtcggcattc cggtggtttc tactctgcat agcggaatac cggaactggt
    21781 ggaggccgat aaatccggct ggctggtgcc tgagaacgat gctcgcgcac tggcgcaacg
    21841 actggcggcg tttagccaac tggacaccga cgaattggct ccggtcgtca aacgtgcgcg
    21901 cgaaaaagtc gaacacgatt ttaaccagca ggtgattaat cgagaactcg ccagcttgct
    21961 gcaagcttta tagaggagaa cgtatgccat ttaaaaaaat ctcccgacgc accttcctga
    22021 cggcaagctc ggtgcttgcc ttcctccata cccctttcgc tcgcgcgctt cccgcccgac
    22081 caagcgttaa cattaacgac tacaacccac acgactggat tgcctcattt aaacaagctt
    22141 ttagtgaagg gcaaacggtc gtcgtgcctg ccggattggt ttgtgacaat atcaacaccg
    22201 gcatcttcat tcctcctggc aaaacgttac acatccttgg aagcctgcgc ggcaacggca
    22261 gagggcgatt tgtcttacag gacggcagcc aggtgacagg ggaggagggc ggcagtatgc
    22321 ataacatcac cctggatgtg cgtggttctg actgcaccat caaagggctg gcgatgagcg
    22381 gctttggtcc ggtaacgcag atctatatcg gcggcaaaaa caaacgggtc atgcacaatc
    22441 tgaccatcga taacctcact gtcagccacg ctaattacgc catcttacgc cagggatttc
    22501 ataaccagat tatcggtgcc aacatcacca attgtaagtt cagtgattta cagggcgacg
    22561 ccattgaatg gaacgtggcg attaacgacc gtgatatttt gatatccgac catgtcatcg
    22621 agcgcatcaa ctgtaccaac ggcaaaatca actggggcat tggcataggc cttgcgggaa
    22681 gcacttacga taataattac ccggaagatc aggcagtgaa aaactttgtc gtggcgaata
    22741 tcacgggatc ggattgtcgg cagttgatcc atgttgaaaa tggtaaacat tttgttattc
    22801 gtaatatcaa agcctgcaat atcacgccgg atttcagtaa gaaagtaggc attgataacg
    22861 ccacagtcgc tatttacggt tgtgacagtt tcgtgattga taatattgaa atgattaata
    22921 gcgccgggat gttaatcggc tatggggtaa ttaaaggcaa atatctctcg ataccgcaaa
    22981 atttccgagt gaataatatt caaatggata acacccatct tgcttataaa ttgcgcggca
    23041 tccaaatctc cgccgggaat gctgtctcct ttgtggcgct gactaacatt gagatgaagc
    23101 gtgcgtcgct ggagttacac aacaaaccgc aacacctttt tatgcgaaat atcaaggtga
    23161 tgcaggaatc ctctgttgga ccagcattga gcatgaactt tgacatgcgc aaagacgttc
    23221 gcggcatttt tatggcgaaa aaagaaacac tgctgtctct tgcaaatgtt catgcggtga
    23281 atgaaaaagg gcaaagctcc gtcgatatcg acagagttaa tcaccatatt gttaatgtgg
    23341 aaaagattaa ctttagattg ccggaacgga gagagtagat ttgcgaccat tcataaactt
    23401 actggatata atgttctaaa agattataat cctcacacta actgttaaag ctggggctgg
    23461 gcagcattga ctactcaggg aaggtactca tctttatctt gtgtttctta cgtaacaacc
    23521 agactaaagt gatttatgaa cgataacgtt ttgctcatag gagcttccgg attcgtagga
    23581 acccgactac ttgaaacggc aattgctgac tttaatatca agaacctgga caaacagcag
    23641 agccactttt atccagaaat cacacagatt ggcgatgttc gtgatcaaca ggcactcgac
    23701 caggcgttag ccggttttga cactgttgta ctactggcag cggaacaccg cgatgacgtc
    23761 agccctactt ctctctatta tgatgtcaac gttcagggta cccgcaatgt gctggcggcc
    23821 atggaaaaaa atggcgttaa aaatatcatc tttaccagtt ccgttgctgt ttatggtttg
    23881 aacaaacaca accctgacga aaaccatcca cacgatcctt tcaaccacta cggcaaaagt
    23941 aagtggcagg cagaggaagt gctgcgtgaa tggtataaca aagcaccaac agaacgttca
    24001 ttaaccatca tccgtcctac cgttatcttc ggtgaacgca accgcggtaa cgtctataat
    24061 ttgctgaaac agatcgctgg cggcaagttt atgatggtgg gcgcagggac taactataag
    24121 tccatggctt atgttggaaa cattgttgag tttatcaagt acaaactgaa gaatgttgcc
    24181 gcaggttatg aggtttataa ttacgttgat aagccagatc tgaacatgaa ccagttggtt
    24241 gctgaagttg aacaaagtct gaacaaaaag atcccttcaa tgcacttgcc ttacccacta
    24301 ggaatgttgg gtggatattg ctttgatatc ctgagcaaaa ttacgggcaa aaaatacgct
    24361 gtcagctctg tgcgcgtgaa aaaattctgc gcaacaacac agtttgacgc aacgaaagtg
    24421 cattcttcag gttttgtggc accgtatacg ctgtcgcaag gtctggatcg aactctgcag
    24481 tatgaattcg ttcatgccaa aaaagacgac ataacgtttg tttctgagta atgcagacga
    24541 gtcagagaaa aaaggaccag gttggactac gattattcgg aaagaggtac tgattaaatt
    24601 atcagtatgc ggaattccga gtaatccgag taacccagat gctgatggtg tgtagtgtta
    24661 tcgtaaggtt taatcaaagt tatgcggaac taagctttgg tatatagcca taacgtttgg
    24721 ctaacctatg gcaataattt acgcactatc agccacttag aggtaggaaa agaatgacga
    24781 atttaaaagc agttataccg gtagcaggtc ttgggatgca tatgttgcct gccactaagg
    24841 cgattcccaa agagatgcta ccgatcgtcg acaagccaat gattcagtac attgttgacg
    24901 agattgtggc tgcagggatc aaagaaatcc tcctggtaac tcacgcgtcc aagaacgcgg
    24961 tcgaaaacca cttcgacacc tcttatgaat tagaatctct ccttgaacag cgcgtgaagc
    25021 gtcaactgct ggcggaagta caatctatct gtccgccggg cgtgaccatt atgaacgtgc
    25081 gtcagggtga acctttaggt ttgggccact ccattttgtg tgcacgcccc gccattggcg
    25141 acaacccatt tgtcgtggtg ctgccggacg ttgttatcga tgatgccagc gccgacccgc
    25201 tgcgctacaa ccttgctgct atgattgcgc gcttcaatga aacgggccgt agtcaagtgc
    25261 tggcaaaacg tatgccgggt gacctctctg aatactccgt catccagacc aaagaaccgc
    25321 tggaccgtga aggcaaagtc agccgcattg ttgaatttat cgaaaaaccg gatcagccgc
    25381 agacgctgga ctcagacatc atggccgtag gtcgttatgt gctttctgcc gatatttggc
    25441 cggaacttga acgcactcaa cctggtgcat gggggcgtat tcagctgact gatgccattg
    25501 ctgaactggc gaaaaaacag tccgttgatg ccatgctgat gacaggtgac agctacgact
    25561 gcggtaaaaa aatgggttat atgcaggcgt ttgtgaagta tggcttacgc aacttgaaag
    25621 aaggggcgaa gttccgtcta tttattcaga atttagtgac aagaagtgat tagtaaaaat
    25681 agtgacaatt cgatggcgaa tattaagcca tcgaattttg aaaggttata a
//
LOCUS       DAZFRN010000065        25619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFRN010000065 DAZFRN010000000
VERSION     DAZFRN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.065"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(23..109)
                     /locus_tag="ACHKUB_002545"
     tRNA            complement(23..109)
                     /locus_tag="ACHKUB_002545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Leu,seq:cag)
     gene            complement(138..224)
                     /locus_tag="ACHKUB_002546"
     tRNA            complement(138..224)
                     /locus_tag="ACHKUB_002546"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Leu,seq:cag)
     gene            complement(525..1556)
                     /gene="rsmC"
                     /locus_tag="ACHKUB_002547"
     CDS             complement(525..1556)
                     /gene="rsmC"
                     /locus_tag="ACHKUB_002547"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO6655550.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1659..2072
                     /gene="holD"
                     /locus_tag="ACHKUB_002548"
     CDS             1659..2072
                     /gene="holD"
                     /locus_tag="ACHKUB_002548"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO6655551.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            2041..2487
                     /gene="rimI"
                     /locus_tag="ACHKUB_002549"
     CDS             2041..2487
                     /gene="rimI"
                     /locus_tag="ACHKUB_002549"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6655552.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2502..3179
                     /gene="yjjG"
                     /locus_tag="ACHKUB_002550"
     CDS             2502..3179
                     /gene="yjjG"
                     /locus_tag="ACHKUB_002550"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO6655553.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3270..4859
                     /gene="prfC"
                     /locus_tag="ACHKUB_002551"
     CDS             3270..4859
                     /gene="prfC"
                     /locus_tag="ACHKUB_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO6655554.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5252..5857
                     /gene="osmY"
                     /locus_tag="ACHKUB_002552"
     CDS             5252..5857
                     /gene="osmY"
                     /locus_tag="ACHKUB_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO6655555.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5984..6145
                     /locus_tag="ACHKUB_002553"
     CDS             5984..6145
                     /locus_tag="ACHKUB_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO6655556.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6267..7340
                     /locus_tag="ACHKUB_002554"
     CDS             6267..7340
                     /locus_tag="ACHKUB_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO6655557.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7337..8119
                     /locus_tag="ACHKUB_002555"
     CDS             7337..8119
                     /locus_tag="ACHKUB_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6655558.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRSEPADEIAEVLLNNTYAVFSVSG"
     gene            complement(8232..9095)
                     /locus_tag="ACHKUB_002556"
     CDS             complement(8232..9095)
                     /locus_tag="ACHKUB_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO6655559.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(9067..10617)
                     /locus_tag="ACHKUB_002557"
     CDS             complement(9067..10617)
                     /locus_tag="ACHKUB_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO6655560.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            10875..11654
                     /gene="deoC"
                     /locus_tag="ACHKUB_002558"
     CDS             10875..11654
                     /gene="deoC"
                     /locus_tag="ACHKUB_002558"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO6655561.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11781..13103
                     /gene="deoA"
                     /locus_tag="ACHKUB_002559"
     CDS             11781..13103
                     /gene="deoA"
                     /locus_tag="ACHKUB_002559"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO6655562.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            13155..14378
                     /gene="deoB"
                     /locus_tag="ACHKUB_002560"
     CDS             13155..14378
                     /gene="deoB"
                     /locus_tag="ACHKUB_002560"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6655563.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14458..15177
                     /gene="deoD"
                     /locus_tag="ACHKUB_002561"
     CDS             14458..15177
                     /gene="deoD"
                     /locus_tag="ACHKUB_002561"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO6655564.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(15338..15601)
                     /locus_tag="ACHKUB_002562"
     CDS             complement(15338..15601)
                     /locus_tag="ACHKUB_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6655565.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(15633..16649)
                     /gene="lplA"
                     /locus_tag="ACHKUB_002563"
     CDS             complement(15633..16649)
                     /gene="lplA"
                     /locus_tag="ACHKUB_002563"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO6655566.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            complement(16677..17321)
                     /locus_tag="ACHKUB_002564"
     CDS             complement(16677..17321)
                     /locus_tag="ACHKUB_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO6655567.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            17427..18395
                     /gene="serB"
                     /locus_tag="ACHKUB_002565"
     CDS             17427..18395
                     /gene="serB"
                     /locus_tag="ACHKUB_002565"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO6655568.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            18444..19826
                     /gene="radA"
                     /locus_tag="ACHKUB_002566"
     CDS             18444..19826
                     /gene="radA"
                     /locus_tag="ACHKUB_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO6655569.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            19847..21079
                     /gene="nadR"
                     /locus_tag="ACHKUB_002567"
     CDS             19847..21079
                     /gene="nadR"
                     /locus_tag="ACHKUB_002567"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO6655570.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21386..23053)
                     /gene="ettA"
                     /locus_tag="ACHKUB_002568"
     CDS             complement(21386..23053)
                     /gene="ettA"
                     /locus_tag="ACHKUB_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO6655571.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            23264..25201
                     /gene="sltY"
                     /locus_tag="ACHKUB_002569"
     CDS             23264..25201
                     /gene="sltY"
                     /locus_tag="ACHKUB_002569"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO6655572.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            25291..25617
                     /gene="trpR"
                     /locus_tag="ACHKUB_002570"
     CDS             25291..25617
                     /gene="trpR"
                     /locus_tag="ACHKUB_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO6655573.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         5959 a         6652 c         7068 g         5940 t
ORIGIN      
        1 ctttttttga tatcgcctcg tttggtgcga ggggggggac ttgaaccccc acgtccgtaa
       61 gaacactaac acctgaagct agcgcgtcta ccaattccgc caccttcgcc cagtgcgagt
      121 aatatcaacg tggtttttgg tgcgaggggg gggacttgaa cccccacgtc cgtaaggaca
      181 ctaacacctg aagctagcgc gtctaccaat tccgccacct tcgcatacca tcaattctta
      241 aaaagaattg ctaccacgga ggcgcattct agtggttttc agcttttcgt caatagttaa
      301 ttatcgccgg aggtgtaatt gctggaaaaa tgtccatcag gaaactagcg tgcaggtttt
      361 gtaggtcgga taaggcgttc acgccgcatc cgacacggta tttgggtgca gatgccagat
      421 gcgacgcggg cgcgtcttat ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg
      481 ataaggcgtt tacgccgcat ccgacacagt attcggcggg atacttaacc tttcttcgcc
      541 tggcgggtca taatggcgcg atacaccttg aagcgcccgg tttgcgcgat aacttcatgg
      601 aagccaaatg tctcatccag cacgtccggg taaggcagga aggcgttcgc tacaattcgc
      661 agctcgccgc cgctattaag atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc
      721 aggctggttt gcatcccatc gtggaacggc gggttggaga tgatcatatc aaaacgacct
      781 ttcacctcgg aaaagacgtt gctggcaaag acttcacctt caacacagtt ggccgcaagt
      841 gttgcgcggc tggcttctac cgctggcgca gagacatcgc acaaggtgag acgaattttc
      901 ggcgaatggc gcgcaaaggc aactgaaagc acccccgcgc cacagccgac atccagcact
      961 ttacctttcg tgtgcggagt taacgtcgag agcagcaact ggctaccgac atccagaccg
     1021 tcgcggctaa acacgccagg cagcgttttg accgtcaggc catcgacgct gtattcgccc
     1081 cagaatttat cggcatcaaa taccggctgt ttttccagac gaccaaaata gaggccacag
     1141 cgacgagcgc tgtcgacttt attcaacggc gcataatctg ccagcatctg ctcggcgctg
     1201 cgcacgccgc tgcggttctc gccaacgaca aaaatatccg tccccaccgg cagcagagaa
     1261 agtaaattca tcaactggaa ctgggcttcc ggtttgttct tcggccagta gtaaatcagc
     1321 gtatcgcaat ctgcgacgtc atccgccgtg gcgaccagac tgaaacgggc gttatccccc
     1381 atctggcggc ttaatacctg ccagtggtgg aattgctggg tatgagcacg gctggccgcg
     1441 gtatctaaac gcgcgggcag gtcatcctgt aagtctccgg caaacagaat acggctttgt
     1501 tcgaaatcat cactgtgacg cagcaagact tcacttgccg gggtaaatgc agacatggaa
     1561 tgctcctcaa ttgatactgg cggcgattat agccatatgt tggcgcggta tcgacgaatt
     1621 tgctatattt gcgcccctga caacaggagc gattcgctat gacatcccga cgagactggc
     1681 agttacagca actgggcatt acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg
     1741 agattgccat tgcgatcccg gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg
     1801 ccctgactga tcctttagtg agcgatgttc tgcgcgcatt aaccgtcagc cccgaccagg
     1861 tgctgcaact gacgccagaa aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct
     1921 ggcggttggg tactgacgaa ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc
     1981 tcaccgattt acgggcaaac ccaacggcac gcgccgcgtt atggcaacaa atttgcacat
     2041 atgaacacga tttcttccct cgaaacgact gatttaccgg cggcttacca cattgaacaa
     2101 cgcgcccacg cctttccgtg gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat
     2161 ctcaactttc agttaacgca aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg
     2221 ctggatgaag ctacattgtt caatattgcg gtcgatcctg actatcagcg tcagggattg
     2281 ggaagggcgc tgctggaaca tctgatcgac gaactggaaa aacgcggcgt ggcgacacta
     2341 tggctggaag tccgtgcttc aaacgctgcc gccattgccc tgtacgaaag tttaggcttt
     2401 aacgaggcga cgattcgccg caattactac cccaccacgg acggtcgcga agacgccatc
     2461 atcatggcgt tgccaatcag tatgtaagac aaggtggaat aatgaagtgg gactggattt
     2521 tctttgatgc cgatgaaacg ctgtttacct ttgactcatt caccggcctg cagcggatgt
     2581 ttcttgatta cagcgtcacc tttaccgctg aagattttca ggactatcag gccgttaaca
     2641 agccactgtg ggtggattat caaaacggcg cgatcacttc attacagctt cagcacgggc
     2701 ggtttgagag ctgggccgaa cggctgaaag ttgaagcagg cttgcttaac gatgccttta
     2761 ttaatgcgat ggcggaaatc tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca
     2821 ttcgtggcaa cgccaaaatc ggcatcatca ccaacggctt tagcgccttg cagcaagtgc
     2881 gtctggaacg cacgggcctg cgtgattact tcgatttgct ggtgatttcc gaagaagttg
     2941 gcgttgccaa accgaataag aaaattttcg attatgcgct ggaacaggcg ggcaatcctg
     3001 accgttcacg cgtgctgatg gttggcgaca ctgccgagtc cgatattctc ggtggcatca
     3061 acgccgggct tgcgacctgc tggctgaatg cgcaccatcg cgagcaacca gaaggcatcg
     3121 cgcccacctg gaccgtttct tcgttgcacg aactggagca gctcctgtgt aaacactgat
     3181 tgcctccccc ccgttgatgg gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg
     3241 cgatgccgct tactcaagaa gaaagaatta tgacgttgtc tccttatttg caagaggtgg
     3301 cgaagcgccg cacttttgcc attatttctc acccggatgc cggtaagact accatcaccg
     3361 agaaggtgct gctgttcgga caggccattc agaccgccgg tacagtaaaa ggccgtggtt
     3421 ccaaccagca cgctaagtcg gactggatgg agatggaaaa gcagcgtggg atctccatta
     3481 ctacgtctgt gatgcagttt ccgtatcacg attgcctggt taacctgctc gacaccccgg
     3541 ggcacgaaga cttctcggaa gatacctatc gtaccctgac ggcggtggac tgctgcctga
     3601 tggttatcga cgctgcaaaa ggtgttgaag atcgtacccg taagctgatg gaagttaccc
     3661 gtctgcgcga cacgccgatc ctcaccttta tgaacaaact tgaccgtgat atccgcgacc
     3721 cgatggaact gctcgatgaa gttgagaacg agctgaaaat cggctgcgca ccgatcacct
     3781 ggccgattgg ctgcggcaag ctgtttaaag gcgtttacca cctttataaa gacgaaacct
     3841 atctctatca gagcggtaaa ggccacacga ttcaggaagt ccgtattgtt aaagggctga
     3901 ataacccgga tctcgacgct gcggttggtg aagatctggc acagcagcta cgtgacgaac
     3961 tggaactggt gaaaggcgcg tctaacgagt tcgacaaaga attgttcctt gcgggcgaaa
     4021 tcactccggt attcttcggt actgcgctgg gtaacttcgg cgttgatcat atgctggatg
     4081 gcctggtgga gtgggctcct gcgccgatgc cgcgtcagac tgatacccgt accgtagagg
     4141 cgagcgaaga caaatttacc ggcttcgtat ttaaaattca ggccaacatg gacccgaaac
     4201 accgcgaccg cgtggcgttt atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc
     4261 tgcgccaggt gcgtactgcg aaagatgtgg ttatctccga cgcgctgacc tttatggcgg
     4321 gcgaccgttc gcacgttgaa gaagcgtatc cgggcgatat cctcggcctg cacaaccacg
     4381 gcactattca gatcggcgac acctttaccc agggtgagat gatgaagttc accggtattc
     4441 cgaacttcgc accagaactg ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac
     4501 agctgctcaa agggctggta cagctttccg aagagggcgc ggtgcaggtt ttccgtccga
     4561 tctccaacaa cgatttgatc gttggtgctg ttggtgtgct gcagtttgat gtggtggtat
     4621 cgcgcctgaa gagcgaatac aacgttgaag cagtatatga atcagtcaac gttgccactg
     4681 cccgctgggt agaatgtgcg gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa
     4741 gccaactggc gcttgatggc ggcgataacc tcgcttacat cgctaccagc atggtcaacc
     4801 tgcgcctggc gcaggaacgt tatcccgacg ttcagttcca ccagacccgc gagcattaat
     4861 tcttgcctcc agggcgcggt agccgctgcg ccctgtcaat ttcccttcct tattagccgc
     4921 ttacggaatg ttcttaaaac attcactttt gcttatgttt tcgctgatat cccgagcggt
     4981 ttcaaaattg tgatctatat ttaacaaagt aatgacattt ctgacggcgt taaataccgt
     5041 tcaatgcgta gatatcagta tctaaagccg tcgattgtca ttctaccgat attaataact
     5101 gattcagagg ctgtaatggt cgttattcat cacccatcgc ttttgtgatg gcgaccattg
     5161 acttctgtag agggtgaagt ctctccctat tcagcaatgc aacctcgtgt tgccaggctc
     5221 aaattacgag caaacataca ggaataaatc gatgactatg acaagactga agatttcgaa
     5281 aactctgctg gctgtaatgt tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa
     5341 caacgcacag actaccaatg aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt
     5401 cggtaatttc atggatgaca gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca
     5461 tgacaacatc aagagcaccg atatctctgt caaaaccgat caaaaagtcg tgaccctgag
     5521 cggtttcgtt gaaagccagg ctcaggccga agaggcagtg aaagtggcga aaggcgttga
     5581 aggggtgacc tctgtcagcg acaaactgca cgttcgcgac gctaaagaag gctcggtgaa
     5641 gggctacgcg ggtgacaccg ccaccaccag tgaaatcaaa gccaaactgc tggcggacga
     5701 tatcgtccct tcccgtcatg tgaaagttga aaccaccgac ggcgtggttc agctctccgg
     5761 taccgtcgat tcccaggcac agagcgaccg tgctgaaagt atcgccaaag cggtagatgg
     5821 tgtgaaaagc gttaaaaatg atctgaaaac taagtaattc gtcgtaattc gtcctcccga
     5881 actttgtcgg gaggcgcgat gtgcaccaca ctaaaaatat cgcattgagt gattttcacg
     5941 ctcatatcaa gcggtgacat taactatggt aaaggagacg cttatgtttc gttggggcat
     6001 catatttctg gttatcgcgt taatcgccgc cgcacttggg tttggtggtc tggccggtac
     6061 cgctgcaggc gcagctaaaa ttgtctttgt cgtcgggatt attctgttcc tggtgagttt
     6121 gttcatgggc cgaaaacgac cctagatttc agaccattaa gaaaatttta tccaaagcca
     6181 gtccagcgga ctggcttttg cggttttagc gaataataaa ttgcgttact ttgtcattct
     6241 ttcacaacaa aaacaggaag gcagaggtgg ggcagcgaat acctgtaacg cttggtaata
     6301 ttgcgccgtt gtcgctaagg ccgttccagc ctggacgaat agctctggtg tgcgaaggcg
     6361 gcggacagcg tggaattttc acggctggcg tgctggatga gtttatgcgc gcgcagttta
     6421 atcctttcga tctttatctc ggcacatctg ccggggcgca gaacctctcg gcgtttatct
     6481 gcaatcagcc cggttacgcg cgcaaagtca tcatgcgcta taccacaaaa cgcgaatttt
     6541 tcgatccatt gcgctttgtc cgtggaggaa atcttatcga tctcgactgg ctggtggagg
     6601 ccaccgcaag ccagatgccg ttgcaaatgg ataccgccgc gcggttgttt gacagcggca
     6661 aatcgtttta tatgtgcgcc tgtcgtcagg atgactacgc gcctaattac tttttaccaa
     6721 ccaaacaaaa ctggctggat gtgattcgcg cctccagtgc gatacctggc ttttatcgta
     6781 gcggagtgtc gctggaaggc attaactacc tggatggcgg gatcagtgat gcgattccgg
     6841 ttaaagaggc ggcaaggcag ggcgctaaaa cgttggtcgt catccgcact gtgccgtcac
     6901 aaatgtacta cacgccgcag tggttcaaac gcatggaacg ctggctgggt gacagtagcc
     6961 tgcagccgct ggtcaatctg gtgcagcatc atgaaaccag ctatcgtgac attcagcaat
     7021 ttattgagaa accaccgggc aagctgcgga tattcgaaat ttatccaccg aagccattac
     7081 atagtatcgc gcttggcagt cggattccgg cgctgcgtga agactataaa cttgggcgtt
     7141 tatgcggtcg ttatttcctc gccacggttg gcaagctatt aactgaaaaa gcgccgctta
     7201 cccgccatct ggtgccagtg gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg
     7261 ccaacgatac gctggttgcc gaagtgagcg atgctccgca ggcgaacgac ccgacattta
     7321 acaatgagga tctggcttga tttgccgttt tatcgatacc cactgccatt ttgatttccc
     7381 gccgtttagt ggcgatgaag aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa
     7441 gatcattgtt ccggcgacag aggcggaaaa ttttgcccgc gtgcaggcgt tagcggaaaa
     7501 atatcaaccg ctttatgccg cattggggtt gcatccgggg atgttggaaa aacatagcga
     7561 tgtgtctctt gatcagctac agcaggcgct ggaaaggcgt ccggcgaagg tggtggcggt
     7621 gggggagatc ggtctggatc tcttcggcga cgatccgcaa tttgagaggc agcagtggtt
     7681 actcgacgaa cagctgaaac tggcgaaacg ctacgatctg ccggtgatcc tgcattcacg
     7741 gcgcacacac gacaaactgg cgatgcatct taaacgccac gatttatcgc gcactggcgt
     7801 ggttcacggt ttttccggca gcctgcaaca ggccgaacgg tttgtacagc tgggctacaa
     7861 aatcggcgta ggcggtacta tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc
     7921 aaaattaccg ctggcatcgt tgttgctgga aaccgacgcg ccggatatgc cgctcaatgg
     7981 ttttcagggg caggctaacc gcccggaaca ggccgcccgg gtatttgccg tgctgtgcga
     8041 gttgcgctcg gaaccggcgg atgagattgc cgaagtgttg cttaataata cgtatgcggt
     8101 gtttagcgtt agtgggtagt ttgcccttcg taggcagcat ccggcagtcg gcgcataatg
     8161 cctgatgcga cgcttgtcgc gtcttatcat gcctacaggt ttgtgccgaa ccgtaggccg
     8221 tatccggcat gtcacaaata gagcgccgga aatatcaacc ggctcacccc gcgcaccttt
     8281 aacgcatcag ccaacggctc aacgtcttcc ggcgtggcgc tcgcccagct ttgcgcctcg
     8341 ccatacacgc cgtgggcatg aaacgcattc aggcgaaccg gaacatcgcc aagtctcttg
     8401 ataaacgtcg ccagctcatc gatgtgatgc aaataatcca cctggtctgg aattaccagc
     8461 aaacgcagtt ccgccagctt accgcgctct gccagcaaac agatgctgcg cttaatctgc
     8521 tgattatcgc gtccggttaa ttgctgatga cattcgctcc cccacgcttt gagatcgagc
     8581 atcgcgccgt cgcacaccgg gagtaatttt tcccagccgg tttcgctcaa cataccgtta
     8641 ctgtccacga ggcaggtgag gtggcgcagt tgcggatcgt ttttgatggc tgtaaacagc
     8701 gccaccacaa acggcagttg ggtcgtggct tcgccgccgc ttaccgttat cccttcaata
     8761 aacagcaccg ctttgcggac atggctaagc acttcgtcca cgctcatgga ttgcgccatt
     8821 ggcgtggcat gttgcggaca cctcttcagg caggtatcac actgctcgca aaccacagcg
     8881 ttccacacca ctttgccgtc aacaatctgc aacgcctgat gcggacactg tggcacgcac
     8941 tccccacagt cattgcaacg tcccatcgtc cacggattgt gacagttttt gcagcgcaga
     9001 ttgcagccct gcaaaaacag agccagacga ctgcctggcc cgtcaacgca ggagaagggg
     9061 ataatcttac tgactaaagc gcatctgctg ttcatggctt atcacgcgcg gctggcgttc
     9121 cagaatacga gtgttgcgtg cggcttcttc gcccagccag gtggtgttgg tgcgtgaacc
     9181 ttcggcgcga tatttttcta agtccgacaa acgcaccata taaccggtaa cgcgaaccag
     9241 atcgttaccg ctgacattgg cggtaaattc acgcattccg gctttaaagg caccgaggca
     9301 aagctgtacc agtgcctgcg ggttacgttt gatggtttcg tcgagcgtca gaatgtcgct
     9361 gatgccggaa taataataag catgatgcgg ggcgacagtt tgcagatggg tgatcggatc
     9421 tggctcatcg ccatacggca aacgcgcgcc cggcgtggtg ccgatatcgg aactgatccc
     9481 cgactgtgcg tgtaacatgg cgcgtttttg ccagccatat ttcacggggg tattggcgac
     9541 aaactccgcc agttgcgcgc tgatgcgata acccacttca tttgcggcgg cttctttacc
     9601 gtagcgcgcg gcaatccctt ctttcttaca cagcaagtta accgcttccg ccagcccata
     9661 catgccaaac attggcacaa aacgttcagg gttaatcagc ccttctttca ccaggaagct
     9721 attctcaaag aagtgtgatt gttgatagag gaattcacac cgcgcatcga tgatggcgat
     9781 ctgctgctgg cagtagtgcg gtagagtgcg cgtaaagaag tcatccagcg attcgctgcg
     9841 ctcggcaatg gctttcaggt taaggcgtac cagcgtgctg ccaccacccg ccagcggcag
     9901 tgagttgtaa cagctcacaa tcccgtagcc cccttttgtg aaaattttat catgcaccgg
     9961 accgttggcg atgtgcggtt tgctacattc acagatgttc ttcgccactt ccagcagcag
    10021 gtcatcaggg gtgatttcag gatcgtagat aaaggtcagg ttcggtgaaa cctgcttcaa
    10081 ctctgcgtct gcacgtaaga tcgcgcgggt aatgggcgaa tcagacgggc cgatattggc
    10141 gtgcataaag gcgtctggca gggttctgtc gaggtaacgc cagaaacgtt ttattcgaac
    10201 atcgatctcg tcttgtgtta gaattctaac atacggttgc aacaacgcat ccagttgccc
    10261 caggtagacc ggcatcgatg tgaccgacgg tacgtggtgg taaagaatgg tcagcagaga
    10321 gagtgcgtca tcaagatctt tcgcgccttc cagctccagc cattcggaac cgttcgccag
    10381 aaaacgggcg taatcgggta agacatagcg cggtttgtac ggcgcatgac cttcaaacat
    10441 atcgcagatt acaccttcat ccagcgcgcg gcgggcttcg gcaggaagct gcggataagg
    10501 cagattgttt tctgcttcca gtgccagaaa atggcgcttc tgctccgggc taagcactgg
    10561 gctggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag tgggcatctt
    10621 cttttccttt tatgccgaag gtgatgcgcc attgtaagaa gtttcgtgat gttcactttg
    10681 atcctgatgc gtttgccacc actgacgcat tcatttgaaa gtgaattatt tgaaccagat
    10741 cgcattacag tgatgcaaac ttgtaagtag atttccttaa ttgtgatgta tatcgaagtg
    10801 tgttgcggag tagatgttag aatactaaca aactcgcaag gtgaatttta ttggcgacaa
    10861 gccaggagaa tgaaatgact gatctgaaag caagcagcct gcgtgcactg aaattgatgg
    10921 acctgaccac cctgaatgac gacgacaccg acgagaaagt aattgctctg tgtcatcagg
    10981 ccaaaactcc ggtcggcaat accgccgcta tctgtatcta tcctcgcttt atcccgattg
    11041 ctcgcaaaac gctgaaagag cagggcaccc cggaaatccg aatcgctacg gtaaccaact
    11101 tcccacacgg taacgatgac atcgaaatcg cgctggcaga aacccgtgcg gcaatcgcct
    11161 acggtgctga tgaagttgac gttgtgttcc cgtaccgcgc gctgatggcg ggtaacgagc
    11221 aggttggttt tgacctggtg aaagcctgta aagaggcttg cgcggcagcg aatgtactgc
    11281 tgaaagtgat catcgaaacc ggcgaattga aagacgaagc gctgatccgt aaagcgtctg
    11341 aaatctccat caaagcgggt gcggacttca tcaaaacctc taccggtaaa gtggctgtga
    11401 acgcgacgcc ggaaagcgcg cgcatcatga tggaagtgat ccgtgatatg ggcgtagaaa
    11461 aaaccgttgg tttcaaacca gcgggcggcg tgcgtactgc ggaagatgcg cagaaatatc
    11521 tcgccatcgc agatgaactg ttcggtgctg actgggcaga tgcgcgtcac taccgctttg
    11581 gtgcttccag cctgctggca agcctgttga aagcgctggg ccacggtgac ggtaagagcg
    11641 ccagcagcta ctaagtaaga tgctttacgc ctaatgcgct gcgcttatca ggcctacgag
    11701 acgtatctac ccgtaggccg gataaggcgt agacgcatcc ggcaaaagcc gcctcatact
    11761 cttttcctcg ggaggttacc ttgtttctcg cacaagaaat tattcgtaaa aaacgtgatg
    11821 gtcatgcgct aagcgatgaa gaaattcgtt tctttatcaa cggtattcgc gacaacacta
    11881 tctccgaagg gcagattgcc gccctcgcga tgaccatttt cttccacgat atgacaatgc
    11941 ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc gactggaaaa
    12001 gcctgcatct gaatggcccg attgttgata aacactccac cggcggcgtc ggcgatgtga
    12061 cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg atgatctccg
    12121 gtcgcggcct cggtcatact ggcggtacgc tcgacaaact ggaatccatc cctggcttcg
    12181 acattttccc ggatgacaac cgtttccgcg aaattattaa agacgtcggc gtagcgatta
    12241 tcggccagac cagctcactg gctccggcgg ataaacgttt ctacgcgacc cgtgatatta
    12301 ccgcaaccgt ggactccatc ccgctgatca ctgcctcgat cctggcgaag aaactggcgg
    12361 aaggtctgga tgcgctggtg atggacgtga aagtgggtag cggcgcgttt atgccgacct
    12421 acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc gctggcgtgc
    12481 gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca ggtaacgcgg
    12541 ttgaagttcg tgaagcggtg cagttcctga cgggtgaata tcgtaacccg cgtctgtttg
    12601 atgtcacgat ggcgctgtgc gtggagatgc tgatctccgg caaactggcg aaagatgacg
    12661 ccgaagcgcg cgcgaaattg caggcggtgc tggacaacgg taaagcggca gaagtctttg
    12721 gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt tgagaactac gcgaagtatc
    12781 tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt gtcagtgaaa
    12841 tggatacccg cgcgctgggg atggcagtgg ttgcaatggg cggcggacgc cgtcaggcat
    12901 ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc gaccaggtag
    12961 acggtcagcg tccgctggct gttatccacg cgaaagacga aaacagctgg caggacgcgg
    13021 cgaaagcggt gaaagcggca attaaacttg ccgataaagc accggaaagc acaccaactg
    13081 tctatcgccg tatcagcgaa taacggtata ctgatctgat catttaaatt tgaagcactg
    13141 agtacggaga acatatgaaa cgtgcattta ttatggtgct ggactcattc ggcatcggcg
    13201 ctacagaaga tgcagaacgc tttggtgacg tcggggctga caccctgggt catatcgcag
    13261 aagcctgtgc caaaggcgaa gctgataacg gtcgtaaagg cccgctcaat ctgccaaatc
    13321 tgacccgtct ggggctggcg aaagcacacg aaggttctac cggtttcatt ccggcgggaa
    13381 tggacggcaa cgctgaagtt atcggcgcgt acgcatgggc gcacgaaatg tcatccggta
    13441 aagatacccc gtctggtcac tgggaaatcg ccggtgtccc ggttctgttt gagtggggat
    13501 atttctccga tcacgaaaac agcttcccgc aagagctgct ggataaactg gtcgaacgcg
    13561 ctaatctgcc gggttacctc ggtaactgcc actcttccgg tacggtcatt ctggatcaac
    13621 tgggcgaaga gcacatgaaa accggcaagc cgattttcta tacctccgct gactccgtgt
    13681 tccagattgc ctgccatgaa gaaactttcg gtctggataa actctatgaa ctgtgcgaaa
    13741 tcgcccgtga agagctgacc aacggcggct acaacatcgg tcgtgttatc gctcgtccgt
    13801 ttattggcga caaagccggt aacttccagc gtaccggtaa ccgtcacgac ctggctgttg
    13861 agccgccagc accgaccgtg ctgcagaaac tggttgatga aaaacacggt caggtggttt
    13921 ctgtcggtaa aattgcagac atctacgcca actgcggtat caccaagaaa gtgaaagcaa
    13981 ctggcctgga cgcgctgttt gacgccacca tcaaagagat gaaagaagcg ggtgataaca
    14041 ccatcgtctt caccaacttc gttgacttcg actcttcctg gggccaccgt cgcgacgtcg
    14101 ccggttatgc tgcgggtctg gagctgttcg accgccgtct gccggagctg atgtctttgc
    14161 tgcgcgatga cgacatcctg atcctcactg ctgaccacgg ttgtgatccg acctggaccg
    14221 gtactgacca cacgcgtgaa cacattccgg tactggttta cggcccgaaa gtaaaaccgg
    14281 gttcactggg tcaccgtgaa accttcgcgg atattggtca gactctggca aaatattttg
    14341 gtacttctga tatggaatat ggcaaagcca tgttctgata atgggtgcgg cttgatgccc
    14401 tcaccccggc cctctcccac agggagaggg tgaaaacatg ttgataagga taaaacaatg
    14461 gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt gatgccaggc
    14521 gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg tgaagtgaac
    14581 aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat ttccgtaatg
    14641 ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat caccgatttc
    14701 ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca cgtaaaactg
    14761 cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg catccgtttt
    14821 aaagatcatg actttgccgc tatcgctgac tttgacatgg tgcgtaacgc ggtagacgcg
    14881 gctaaagcac tgggcgttga tgctcgcgtt ggtaacctgt tctccgctga cctgttctac
    14941 tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg gcatcctcgg cgtggaaatg
    15001 gaagctgctg gtatctacgg cgtcgctgca gaatttggcg cgaaagccct gaccatctgc
    15061 accgtgtctg accacatccg cactcacgag cagaccactg ccgctgagcg tcagaccacc
    15121 ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc taggcgataa agagtaattg
    15181 tgtttcgctg aaaggcgatt gtcgtgttaa gccggagtgg gggactgctc cggcttttta
    15241 gtatctattc atcaatagca actgatttat agatttacta ttatttttca tatggataga
    15301 aaataaaata cattcaagta tctattcacg aatctattca tttatgttca gccgtcatcc
    15361 aggaagggca tatcagcaac tctggtggac aaccataaat ttctgccatt ttgcttttga
    15421 tgtttgagct aagccgttcc gtcttttcga tctgttttag agaattaatc gagataccta
    15481 acattttact tacttcagca agcgataatt tatgcctgat acgcacggcg gctagccagg
    15541 aaatacgctg gctgaacatt aaattgataa tctcccgttc ctggttttcc agatcgatca
    15601 tatttccttc cctggatatt ttaatcagcg agttaccgca ccgcccccgc aatccacgtc
    15661 gataactccc gtagctcttt ttcctgttcc gggaagtcaa ccaacagcgc ttcgcactcc
    15721 tgttgcagca tatctgcgcg gtacaggcag ccttgcagtc gtccggcgag ggcttccagc
    15781 ggcgcggggt tgaggctgtc ggtaaacacc tgggcgcggg tgatatggcc tttttcaacg
    15841 tcgaaatgca gttccacgcc gccccagcta aagcgttcat ccagcagatg cgagaatgca
    15901 ggagcctgac cgaagttcca ttcccagcta ctctgacggg caaaggtttc ggcgaagttt
    15961 ggcaagtctg gcgttttgtc cggggagatg atttccgctt ctacacgctc gccataatgg
    16021 gcgaaaaagg ccttggttat ggcctcgcaa acctgctcat gggtgatccc cggcagcagc
    16081 tcggtgaggt tggtcacgcg ggaacgcact gaggtaatgc ctttcgccgc cagtttcttt
    16141 ttatccggat tgagatagtt tgccaggcgg ctaaggtcgg cattgagcag caaggtgccg
    16201 tggtggaagc cacgatcttt ggtttcgcga taggccgatc ctgagacttt gcggtcacct
    16261 tcggcggttt tcaccaccag atcgttacgc ccggacgctt cggcgctgac gccaagcgcg
    16321 ttcagcgcat tgagcacaat cgacgtggag atggttttat cgtactccgg cttgccagcc
    16381 ataaaggtaa agcaggtatt gccgagatcg tggaacaccg cgccgccacc gctactgcgc
    16441 cgtgccaggc ggacgttatc ttcttccatc cgccgggtat tacactcttt ccacgggttc
    16501 tgcgcgcgac caatcactac cgtgtcggca ttgcgccaga gaaacagaac gcgctgcgtg
    16561 gcgggcatct ggcgaaaaat acactcctcc accgccaggt taaaccacgg gtcgtaagag
    16621 tcagagatga gcaggcgtaa tgtggacata acgatttcct ttcttgtagt aatgggtcac
    16681 tcttttttct cgctttcttc ctcttccggc accggtttgc tggcggttaa caggaagggc
    16741 gattgctgcc agcgggtacg tttaccctgt agcagcgtgc gggtcagcac tacgccgatt
    16801 gccagtgaaa gcagcaacat caggcgtaaa atgttagtgg tgttatccac ctgttgggct
    16861 tcggtggcga gcgtatgggt gtcgagcgtc aggcgcagat agccgagcgg tccgtttttc
    16921 ccggcaattg gttcgacaat ctgctggtta aaatagccgc ccgctttttt accgtcgagc
    16981 gccagccggt cgcgcacttc gacgctttcg ccagaacgtg cgataagatc gccttgttcg
    17041 tcatacacac ccgcgtcgag gatacggctt tcatccgtta actgatcgag gatcgcctga
    17101 atgcgttttt catccggtga gtcggtacgc atcagcggtg caacgttcag cgttacctga
    17161 cgcgccaggg tgcgggccag ttcttccagc tgtggattac gttgtcgctg gtggttttga
    17221 ctaaaccatg acgctccctg catcagcgcc actaacaagg caagacagaa caggacaatc
    17281 actgcccgat gcagccggaa tttcagtttt gtgcgagcca tcttccaccc tttgaaaatt
    17341 tgagacttaa tgttgccaga agcaatggat acaaggtagc ctcatgcgtt attttccctg
    17401 cttcgaacga ttttacagga gccttaatgc ctaacattac ctggtgcgac ctgcctgaag
    17461 atgtctcttt atggcctggt ctgcctcttt cattaagtgg tgatgaagtg atgccactgg
    17521 attaccacgc aggtcgtagc ggctggctgc tgtatggtcg tgggctggat aaacagcgtc
    17581 tgacccaata ccagagcaaa ctgggtgcgg cgatggtgat tgttgccgcc tggtgcgtgg
    17641 aagattatca ggtgattcgt ctggcaggtt cactcaccgc acgggctacg cgcctggccc
    17701 atgaagcgca actggatgtc gccccgctgg ggaaaatccc gcacctgcgc acgccgggtt
    17761 tgctggtgat ggacatggat tccaccgcca tccagattga atgtattgat gaaattgcca
    17821 aactggccgg aacgggcgag atggtggcgg aagtaaccga acgggcgatg cgcggcgaac
    17881 tcgattttac cgccagcctg cgcagccgtg tggcgacgct gaaaggcgct gacgccaata
    17941 ttctgcaaca ggtgcgtgaa aatctgccgc tgatgccagg cttaacgcaa ctggtgctca
    18001 agctggaaac gctgggctgg aaagtggcga ttgcctccgg cggctttact ttctttgctg
    18061 aatatctgcg cgacaagctg cgcctgaccg ccgtggtagc caatgaactg gagatcatgg
    18121 acggtaaatt taccggcaat gtgatcggcg acatcgtaga cgcgcagtac aaagcgaaaa
    18181 ctttgactcg cctcgcgcag gagtatgaaa tcccgctggc gcagaccgtg gcgattggcg
    18241 atggagctaa tgacctgccg atgatcaaag cagcagggct ggggattgcc taccatgcca
    18301 agccaaaagt gaatgaaaag gcggaagtca ccatccgtca cgctgacctg atgggggtat
    18361 tctgcatcct ctcaggcagc ctgaatcaga agtaattgct cgcccgccat cctgcgggcg
    18421 gcacagcatt aacgaggtac accgtggcaa aagctccaaa acgcgccttt gtttgtaatg
    18481 aatgcggggc cgattatccg cgctggcagg ggcagtgcag tgcctgtcat gcctggaaca
    18541 ccatcaccga ggtgcgtctt gctgcgtcgc caacggtggc gcgtaacgag cgtctcagcg
    18601 gctatgccgg tagcgccggg gtggcaaaag tccagaaact ctccgatatc agccttgaag
    18661 agctgccgcg tttttccacc ggatttaaag agttcgaccg cgtactaggc ggcggcgtgg
    18721 tgccaggaag tgccattctg attggcggta accctggtgc ggggaaatcc acgctgttgc
    18781 tgcaaacgct gtgcaaactg gcccagcaga tgaaaacgct gtatgtcact ggcgaagagt
    18841 cgctgcaaca ggtggcaatg cgcgctcatc gccttggcct gccgactgac aatctcaata
    18901 tgttgtcgga aaccagcatc gaacagatct gcctgattgc cgaagaagag caaccgaaac
    18961 tgatggtaat tgactcgatc caggtgatgc atatggcgga tgtacaatca tcgcctggta
    19021 gcgtggcgca ggtgcgtgaa acggcggctt atctgacgcg cttcgccaaa acgcgcggtg
    19081 tggcgattgt catggttggg cacgtaacca aagatggctc gctggctggc cctaaagtgc
    19141 tggaacactg tatcgactgt tcggtgcttc tggatggtga tgccgactcc cgttttcgca
    19201 ccttgcgcag ccataaaaac cgcttcggcg cggtgaatga gctgggcgtc ttcgcgatga
    19261 ccgaacaggg gctgcgtgaa gtcagcaacc cttcggcaat tttcttaagt cgcggagatg
    19321 aagtgacctc cggcagctcc gtgatggtgg tgtgggaagg aacgcgtccg ttgctggtgg
    19381 agattcaggc gctggtcgat cactcgatga tggcgaatcc gcgccgcgtg gcagtggggc
    19441 tggaacaaaa ccgtctggca atcctgctgg ctgtcttgca ccgtcacggt ggtctgcaaa
    19501 tggccgatca ggatgtattt gtgaacgtgg tcggcggcgt gaaggtaacc gaaaccagtg
    19561 ccgatttagc gttactgctg gcgatggttt ccagcctgcg tgacagaccg ctgccacagg
    19621 atctggtggt gtttggtgaa gtcgggctgg caggggagat ccgcccggtg cccagcggtc
    19681 aggaacgaat ttcagaagcg gcgaaacacg gttttcgccg ggcgattgtt ccggcggcta
    19741 acgtaccgaa aaaagcgccg gaagggatgc agatttttgg cgttaaaaaa ctctccgacg
    19801 cgcttagcgt gttcgacgac ttataatgag agatacggag ggagatatgt cgtcatttga
    19861 ttacctgaaa actgccatca agcaacaggg ctgcacgcta cagcaggtag ctgatgccag
    19921 cggtatgacc aaagggtatt taagccagtt actgaatgcc aaaattaaaa gccccagcgc
    19981 gcaaaagctg gaggcgttgc accgtttttt ggggcttgaa tttccccgac agaagaaaac
    20041 cattggtgtc gtattcggta agttctaccc gctgcatacc ggacatatct accttatcca
    20101 gcgcgcctgt agccaggttg acgagctgca tatcattatg ggttttgacg atacccgcga
    20161 ccgcgcgttg tttgaagaca gcgccatgtc gcagcagccc acggtgccgg atcgtctgcg
    20221 ctggttatta caaactttta aatatcagaa aaatattcgc attcatgctt tcaacgaaga
    20281 gggcatggag ccgtatccgc acggctggga tgtgtggagc aacggcatca aaaagtttat
    20341 ggctgaaaag gggattcagc cggacctgat ctacacctcg gaagaagccg atgcgccaca
    20401 gtatatggaa catctgggga tcgatacggt gctggtcgat ccgaaacgta cctttatgag
    20461 tatcagcggt gcgcagatcc gcgaaaaccc gttccgctac tgggaatata ttcctacgga
    20521 agtgaagccg ttctttgtac gtaccgtggc gatccttggt ggcgagtcga gcggtaaatc
    20581 caccctggta aacaaacttg ccaatatctt caataccacc agtgcgtggg aatatggtcg
    20641 cgattatgtc ttttcacacc tcggcggtga tgagatcgca ttgcagtatt ccgattacga
    20701 taaaatcgcg ctgggccacg cacaatacat tgattttgca gtgaaatatg ccaataaagt
    20761 ggcgtttatt gacactgatt ttgtcactac ccaggcgttc tgcaaaaagt acgaagggcg
    20821 tgagcatccg ttcgtacagg cgttgattga tgaataccgt ttcgatctgg tgatcctgct
    20881 ggagaacaac acgccgtggg tggcggatgg tttacgcagc ctcggcagtt cggtggatcg
    20941 caaagagttc cagaacttgc tggtggagat gctggaagag aacaatatcg aattcgtgcg
    21001 ggttgaagag gacgattatg acagccgttt cctgcgctgc gtggagctgg tgcgggagat
    21061 gatgggggag cagagataac cgcgatgaaa cggctcaaag gcgaggtata aaataagttt
    21121 tttgatgagt tgaaaacctc tctggaagag gctgtcgaga ttaaacaagg tttaaaagaa
    21181 tcagcacggg tgacccgcta cgaatttgcg gatactaagg ctgttgtagg ccggaaaagg
    21241 cgttcacgcc gtatccggca ttcggtgctc aatgcctgat gcgacgctta ccgcgtctta
    21301 tcaggcctgg agattcacaa cgtattgaaa tttcttggtt tgtaggccgg ataaggcgtt
    21361 cacgccgcat ccggcatttt acgcattact tcgcaatacg cttgtacttg atacgcttcg
    21421 gctccagtgc gtctgcgccc agcgtgcgtt tcttgtactc ttcgtactcg gtaaagttac
    21481 cttcgaagaa ctcaacttta ccttcatcct ggtaatccag aatgtgcgtg gcgatacggt
    21541 cgaggaacca acggtcgtgc gagataacca tcgcgcagcc cgggaactcc agcagggcgt
    21601 tttccagcgc gcgcagggtt tcgatatcca ggtcgttggt tggttcgtcg agcagcagca
    21661 tgttgccgcc aacctgcagc agcttcgcca gatgcagacg accgcgctca ccaccggaaa
    21721 gttcaccaac gcgtttaccc tgatcaaccc ctttaaagtt aaagcggcca acgtaggcgc
    21781 ggcttggcat ctcggtgttg ccgatcttca taatatccag cccgccggaa acttcttccc
    21841 aaacggtttt gctgttatcc attgagtcac ggaactgatc aaccgatgcc agtttcaccg
    21901 tttcacctaa agtgatggtg ccgctgtccg gctgttcctg accagagatc atacggaaca
    21961 gggtcgattt acccgcgccg ttcggaccga tgatcccgac gattgcccct ttcgggatcg
    22021 agaagctcag gtcatcaatc agcaggcgat caccgtagga tttacgcagg ttgctgactt
    22081 ccagcacttt atcgcccaga cgcggtccag gtggaataaa cagttcgttg gtttcgttac
    22141 gtttctgata ttcggtgctg ttcagctctt caaagcgtgc cagacgtgct ttacctttcg
    22201 actggcggcc tttagttccc tgacgtaccc actccagctc tttctcgatc gacttacgac
    22261 gcgccgcttc ttgtgaagct tcctgcgcca ggcgctgatc tttctgctcc agccaggagg
    22321 agtagttgcc ttcccacgga ataccttcac cgcggtcaag ttcgaggatc cagcctgcaa
    22381 cgttatcgag gaagtaacgg tcgtgggtaa tcgccaccac ggtgccctcg aagtcgtgca
    22441 ggaagcgttc cagccaggcc acggattcgg catccaggtg gttggtcggt tcgtcgagca
    22501 gcagcatgtc tggtttttcc agcagcaggc ggcacaacgc tacgcgacga cgctcaccac
    22561 cggagaggtt agcgattttc gcgtcccaat ctggcagacg cagcgcatcc gccgcacgct
    22621 ccagctgtac gttcaggtta tgaccgtcgt gagcctgaat gatctcttcc agacggcctt
    22681 gttcagcggc cagcttgtca aaatcggcat ccggatcggc gtacagcgca tacacttcat
    22741 ccaggcgttt cagggcgtta accacttcag aaaccgcttc ttcaatggac tcacgcacgg
    22801 tgtgttccgg gttcagctgc ggttcctgcg gcaggtaacc aatcttgatg tctggctgcg
    22861 gacgcgcttc accttcgatg tctttatcaa tgcccgccat aatgcgcagc agggtagact
    22921 tacccgcgcc gttcagaccc aggacaccaa tttttgcccc agggaagaaa ctcagagaga
    22981 tgtttttcaa aatatgacgt ttcggcggaa caactttgcc gacacgatgc atggtataaa
    23041 cgaattgagc cacgttggac ttcgcctcta tgtttatcgt gatgatgagt tttcaaaggc
    23101 gaagtgtagc ctttttcccc gcctaatccc agttggtcga tcaccttcgc agtaaaagta
    23161 aaaaagtgtc cgtaacgtgg cgtaaacggc aatgactggt tagcataaat ctattacgcg
    23221 gcatgacgct gcattgatgt atttacactt agaggatgcg cttgtggaaa aagccaaaca
    23281 agttacctgg cggctgttgg ctgccggtgt ctgtctgctg acggtcagca gcgtggcgcg
    23341 agccgactca ctggatgagc agcgtagccg ttacgcgcaa atcaagcagg cctgggataa
    23401 tcgacaaatg gatgtggtcg aacaaatgat gcctggactg aaggattatc cgctttatcc
    23461 ctacctggaa taccgccaga tcaccgatga tctgatgaat caaccggcgg tgacggtcac
    23521 taactttgtt cgcgctaacc ccacgcttcc tcccgctcgc acgctgcaat ctcgtttcgt
    23581 caatgaactg gcgcggcgtg aagactggcg tggcttgtta gcctttagcc cggaaaagcc
    23641 cggaactacc gaagcgcaat gtaattacta ctatgcgaaa tggaacaccg ggcagagtga
    23701 agaagcctgg caaggggcga aagagctgtg gctaaccggc aagagccagc ctaacgcctg
    23761 tgacaagtta tttagcgtct ggcgtgcgtc aggtaaacaa gatccgctgg cgtatttaga
    23821 gcgtatccgt ctggcgatga aagcgggtaa cactggcctg gtaacagtgc tggcagggca
    23881 gatgcctgcc gattaccaga ctatcgcctc ggcaatcatt tcactggcga acaaccctaa
    23941 tacggtactg accttcgcgc gtacaaccgg agcgaccgat tttacccgtc aaatggcagc
    24001 ggtggcgttt gccagtgtgg cgcggcagga tgctgaaaat gcacggctga tgatcccgtc
    24061 gcttgcccag gcgcagcagc ttaatgaaga tcagattcag gagctgcgcg atatcgtcgc
    24121 ctggcgtttg atgggcaacg atgtcaccga cgagcaggcg aaatggcgcg atgacgccat
    24181 tatgcgctcg caatccacgt cgttgattga acgccgtgtg cgaatggcac ttggcaccgg
    24241 cgatcgtcgc ggcctgaata cctggctggc gcgtctgccg atggaagcaa aagagaaaga
    24301 tgaatggcgt tactggcagg cggatttatt gctggaacgc ggacgtgaag ctgaagcgaa
    24361 agagattttg catcaactca tgcaacagcg tggtttctac ccgatggttg cagcacaacg
    24421 catcggagaa gagtatgagc tgaagattga taaagcgccg cagaatgttg acagcgccct
    24481 gactcagggg ccggagatgg cgcgcgtgcg cgagttgatg tactggaatc tcgataacac
    24541 cgcgcgtagc gagtgggcca atctggtgaa gagcaagtca aaaacagagc aggctcaact
    24601 ggctcggtat gctttcaata accaatggtg ggatcttagc gttcaggcaa cgatcgccgg
    24661 gaagctttgg gatcatctgg aagagcgatt cccgctggct tacaacgatc ttttcaaacg
    24721 ttacaccagt gggaaagaga tcccgcaaag ctatgcgatg gcgattgccc gtcaggagag
    24781 cgcctggaat ccgaaagtga aatcaccggt aggggccagc ggcctgatgc agattatgcc
    24841 tggtacagcg acccatacgg tgaagatgtt ctctattcct ggttatagca gccctgggca
    24901 attgctggat ccggaaacga atatcaacat tggcaccagt tacctgcaat atgtttatca
    24961 gcagtttggc aataaccgta ttttctcctc agcagcttat aacgccggac ctggacgggt
    25021 gcgaacctgg cttggcaaca gcgccgggcg tatcgacgct gtggcatttg tcgagagtat
    25081 tccgttctcc gagacgcgcg gttatgtgaa gaacgtgctg gcttatgacg cttactaccg
    25141 ctatttcatg ggggataaac cgacgttgat gagcgccacg gaatggggac gtcgttactg
    25201 atccgcacgt ttatgatatg ctatcgtact ctttagcgag tacaaccggg ggaggcattt
    25261 tgcttccccc gctaacaatg gtgacatatt atggcccaac aatcacccta ttcagcagcg
    25321 atggcagaac agcgtcacca ggagtggtta cgttttgtcg acctgcttaa gaatgcctac
    25381 caaaacgatc tccatttacc gttgttaaac ctgatgctga cgccagatga gcgcgaagcg
    25441 ttggggactc gcgtgcgtat tgtcgaagag ctgttgcgtg gcgaaatgag tcagcgtgag
    25501 ttaaaaaatg aactcggcgc gggcatcgcg acgattacgc gtggatctaa cagcctgaaa
    25561 gccgcgcccg tggagctgcg ccagtggctg gaagaggtgt tgctgaaaag cgattgatt
//
LOCUS       DAZFRN010000066        25455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFRN010000066 DAZFRN010000000
VERSION     DAZFRN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.066"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            240..659
                     /locus_tag="ACHKUB_002571"
     CDS             240..659
                     /locus_tag="ACHKUB_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6655574.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            659..1927
                     /gene="umuC"
                     /locus_tag="ACHKUB_002572"
     CDS             659..1927
                     /gene="umuC"
                     /locus_tag="ACHKUB_002572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO6655575.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKCAMLSPRYTTRSSDLLRVK"
     gene            complement(1973..2503)
                     /gene="dsbB"
                     /locus_tag="ACHKUB_002573"
     CDS             complement(1973..2503)
                     /gene="dsbB"
                     /locus_tag="ACHKUB_002573"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO6655576.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2649..4190)
                     /gene="nhaB"
                     /locus_tag="ACHKUB_002574"
     CDS             complement(2649..4190)
                     /gene="nhaB"
                     /locus_tag="ACHKUB_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO6655577.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNRLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4412..5131
                     /gene="fadR"
                     /locus_tag="ACHKUB_002575"
     CDS             4412..5131
                     /gene="fadR"
                     /locus_tag="ACHKUB_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO6655578.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5183..6715)
                     /locus_tag="ACHKUB_002576"
     CDS             complement(5183..6715)
                     /locus_tag="ACHKUB_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO6655579.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7045..8343
                     /gene="dadA"
                     /locus_tag="ACHKUB_002577"
     CDS             7045..8343
                     /gene="dadA"
                     /locus_tag="ACHKUB_002577"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO6655580.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8353..9423
                     /gene="dadX"
                     /locus_tag="ACHKUB_002578"
     CDS             8353..9423
                     /gene="dadX"
                     /locus_tag="ACHKUB_002578"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO6655581.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9809..11545)
                     /locus_tag="ACHKUB_002579"
     CDS             complement(9809..11545)
                     /locus_tag="ACHKUB_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO6655582.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11640..12554)
                     /gene="ldcA"
                     /locus_tag="ACHKUB_002580"
     CDS             complement(11640..12554)
                     /gene="ldcA"
                     /locus_tag="ACHKUB_002580"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO6655583.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12654..13265
                     /gene="emtA"
                     /locus_tag="ACHKUB_002581"
     CDS             12654..13265
                     /gene="emtA"
                     /locus_tag="ACHKUB_002581"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO6655584.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13267..14001)
                     /locus_tag="ACHKUB_002582"
     CDS             complement(13267..14001)
                     /locus_tag="ACHKUB_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO6655585.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14202..14456
                     /locus_tag="ACHKUB_002583"
     CDS             14202..14456
                     /locus_tag="ACHKUB_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6655586.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14634..15074
                     /gene="ycgY"
                     /locus_tag="ACHKUB_002584"
     CDS             14634..15074
                     /gene="ycgY"
                     /locus_tag="ACHKUB_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO6655587.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15153..16850)
                     /gene="treA"
                     /locus_tag="ACHKUB_002585"
     CDS             complement(15153..16850)
                     /gene="treA"
                     /locus_tag="ACHKUB_002585"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6655588.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17170..18588)
                     /gene="dhaM"
                     /locus_tag="ACHKUB_002586"
     CDS             complement(17170..18588)
                     /gene="dhaM"
                     /locus_tag="ACHKUB_002586"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO6655589.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVVPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18599..19231)
                     /gene="dhaL"
                     /locus_tag="ACHKUB_002587"
     CDS             complement(18599..19231)
                     /gene="dhaL"
                     /locus_tag="ACHKUB_002587"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO6655590.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19242..20312)
                     /gene="dhaK"
                     /locus_tag="ACHKUB_002588"
     CDS             complement(19242..20312)
                     /gene="dhaK"
                     /locus_tag="ACHKUB_002588"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO6655591.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20540..22459
                     /gene="dhaR"
                     /locus_tag="ACHKUB_002589"
     CDS             20540..22459
                     /gene="dhaR"
                     /locus_tag="ACHKUB_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO6655592.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22559..25444)
                     /locus_tag="ACHKUB_002590"
     CDS             complement(22559..25444)
                     /locus_tag="ACHKUB_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6655593.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTSEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
BASE COUNT         6284 a         6332 c         6572 g         6267 t
ORIGIN      
        1 atatcaaaca atgtttaatg tcattatggc gaatgcttct attctatttt tttgccgggt
       61 gatatttttc atttctgctg gatgagcgtc gtcgccagaa ggccacgtga gcacaggata
      121 agagaacgaa aaatcagcag tctatgcagc gacaaatatt gatagcctga atcagtattg
      181 atctgctggc aagaacagac tactgtatat aaaaacagta taacttcagg cagattatta
      241 tgttgtttat caagcctgcg gatctccgcg aaattgtgac ttttccgcta tttagcgatc
      301 ttgttcagtg tggctttcct tcaccggcag cagattacgt tgaacagcgc atcgatctga
      361 atcaactgtt gatccagcat cccagcgcga cttacttcgt caaagcaagt ggtgattcta
      421 tgattgatgg tggaattagt gacggtgatt tactgattgt cgatagcgct attaccgcca
      481 gccatggtga tattgtcatc gctgctgttg acggcgagtt tacggtgaaa aaattgcaac
      541 tacgcccgac ggtacagctt attcccatga acagtgcgta ctcgcccatt accatcagta
      601 gcgaagatac gctggatgtc tttggtgtgg tgatccacgt cgttaaggcg atgcgctgat
      661 gtttgctctc tgtgatgtaa acgcgtttta tgccagctgt gagacggtgt ttcgccctga
      721 tttatggggt aaaccggtgg ttgtgctatc gaataatgac ggttgcgtta tcgcccgaaa
      781 cgctgaggca aaggcgctgg gtgttaaaat gggcgatccc tggttcaaac aaaaagatct
      841 gtttcgtcgc tgtggcgtgg tttgctttag cagcaattat gagctttacg cagacatgag
      901 caatcgggtg atgtcgacgc tggaagaact atcgccccgc gtcgagattt acagtattga
      961 tgaggcattc tgcgatctga caggtgtgcg taattgtcgc gatctgactg attttggcag
     1021 agaaattcgc gcaacggtgc tacaacgtac ccatcttact gttggtgtgg ggatcgccca
     1081 gaccaaaacg ttggctaagc ttgccaatca tgcggcaaaa aaatggcagc ggcagacggg
     1141 tggggtggtg gatttatcaa atctggaacg ccagcgtaaa ttaatgtctg ctctccccgt
     1201 ggatgacgtc tgggggattg gacggcggat cagcaaaaaa ctggacgcga tggggatcaa
     1261 aaccgttctc gatttggcgg atacagatat ccggtttatc cgtaaacatt ttaatgtcgt
     1321 gctcgaaaga acggtgcgtg aactgcgcgg cgaaccctgt ttgcaactgg aagagtttgc
     1381 accgacgaag caggaaatta tctgttcccg ctcgtttggt gaacgcatca cggattatcc
     1441 atcgatgcgg caggccattt gtagttacgc tgcccgggcg gcggaaaaac ttcgcagcga
     1501 gcatcaatat tgtcggttta tctccacatt tattaagacg tcaccatttg cgctcaatga
     1561 accttattac ggcaatagcg cgtcggtaaa actgctgacg cccactcagg acagcaggga
     1621 tatcattaac gctgctacgc gatctctgga tgccatctgg caagcgggcc atcgctacca
     1681 aaaagcgggc gtgatgctgg gggatttctt cagtcaggga gtcgcgcagc tcaatttatt
     1741 cgatgacaac gcaccgcgcc ccgggagtga gcaattgatg acggtaatgg atacactgaa
     1801 tgctaaagag ggcagaggaa cactctattt tgccgggcag gggatccagc aacaatggca
     1861 gatgaagtgt gccatgcttt caccacgtta tacaacgcga agttctgatt tactgcgggt
     1921 caaataaata tagcggcagg aaaaaagcgc tcccgcagga gcgctgaagg gattagcgac
     1981 cgaacaggtc acgttttttc gctttaaacg gctgggaaat caccaccagc accgcgacaa
     2041 tcaggtaagc gataaaaata ccgagcagcc actgcggcat ttccagacct aaaaaatccc
     2101 actgacgctc ggcacaatcg ccagaggcga caaacacttg cggcacccat ttatccagcg
     2161 gcagccattc cgggaaacga accataaaat cacaggtggc aaacggcgaa ggatagagct
     2221 gaagcatggt gtgctcgtaa gttaactgca caccgcggaa cgcactatac aaccagataa
     2281 ccatcgctac ataacgcagc ggagttttcg gggcgatcgc gccaatcagc gcagcaccca
     2341 gaacgccgaa taacgcacaa cgttcataaa tacagagcac gcaaggtttc agtaacatca
     2401 catgctggaa ccacagcgcc gtcagttcca gtgccagagc agtaaacgcc atcaacagcc
     2461 acgcgccccg gccttgtgaa cattggttca aaaatcgcaa cataatcatt tccctgcaat
     2521 atgcatagag tgcgcagttt aaaccaattc attcgctgcg ccaccaggag gcagcaaaaa
     2581 taggaagcaa ttggacaatt atcgggcgaa aaggcaaacc gggcatttat atgcccggta
     2641 agttgttatc aaagcgttgc tatccagccc atttgcataa accattcggt tacaggggca
     2701 agcgtaaact cgacgcagag caagccgacg agtgtcagta cgagggtgta aggcagggcc
     2761 atccacacca tgcggccata agagaggcga atcaatggcg cgagtgcaga ggtcagcagg
     2821 aacaggaacg cagcctgacc gttcggcgta gcgacggagg gcagattggt accggtatta
     2881 atggcgaccg ccagcagctc gtattgcttc aacgtgatag cgccactttc cattgccgct
     2941 ttcgcttcgt tgatataaat cgtccccacg aagacgttat ccgaaatgga tgacagcaga
     3001 ccgttgaaaa tatagaacag cgacagctga gcatgttccg atgcctgcaa cacaaactga
     3061 ataattggcg aaaacagttg ttggtcgata atcaccgcga ctaccgagaa aaacaccgtc
     3121 aacagtgcgg tgaatggcag agattcggtg aaggctttac cgatagcatg ttcatcggtg
     3181 acaccggtca atgatgtcgc cagaataatg actgacaaac caatcaagcc aacttccgcc
     3241 agatgcaacg ccagcgcagt caccagccag acgccaataa tcgcctggac aatcagacgg
     3301 attttatcct gacgggtacg ctggtggcgg ctttgatcgt caaactgttg caacacttcg
     3361 cggactttct ccggcagcgt ttcaccgtag ccaaaccaac gcagcttctc taccagcagg
     3421 caggttaaca ggccacaaat cagaaccgga acggtcaccg gcgacatgcg caggaagaaa
     3481 tcgccaaaat gccagccagc cgctttagcg atgatcaggt tctgtggttc gcccaccatg
     3541 gtcatcacgc cgcctaatgc agtgccgaca ccggcatgca tcatcaggct acgcagaaag
     3601 ccacggaact gttccagaac cactttgtaa tgcttgtcga tatgactatc gtcttgcagg
     3661 tcggtatctt cggtacggga agaggctacg cggtgataaa taccataaaa accgacagca
     3721 acgctgatca ccaccgccac gacggttaag gcatcgagga acgcggagag gaacgctgcc
     3781 gccacgcaaa aagagagcga cagcagcatt ttggagcgaa tgcttaacag caaacgggta
     3841 aatatgaaca gcaacagctg tttcataaaa tagatacccg ccaccataaa catcaataac
     3901 agcaagactt caagatttgc cgccacctct tcacggacgt gttccgcgct ggtcatgccg
     3961 atgaataccg cttcgatagc caacagacca ccggggagca gcgggtagca tttcagggcc
     4021 atcgccagag tgaaaataaa ttccgctacc agcaaccagc ccgcgacgaa agggctgatg
     4081 aggaaaatta accggtttac gattaagaaa atgatgaggg cgagtttgta ccagtcgggg
     4141 gactggccca aaaaattgcg ccatagcgcg cggccccagg agatctccat gatggtttcc
     4201 cttaccttac aaataatcaa tgatgttttt atgtttaaac gcaaagctta acggtcaggc
     4261 aggagtgagg caagtcttga tagtcaaggg gaaagagatg cggaaaatga agccttgatc
     4321 ccttttttct tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa
     4381 ctttgttttg ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg
     4441 tttcgcggaa gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat
     4501 tttgcccgca gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga
     4561 agtgttacag cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa
     4621 ggtgaataat ttctgggaaa cctctggttt aaatatcctt gaaacactgg cgcgactgga
     4681 tcacgaaagt gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac
     4741 tatttttatt cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac
     4801 cgctaatgaa gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg
     4861 cggcctggcg tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaagg
     4921 gctgtatacg cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct
     4981 gggcttctac cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga
     5041 aacagtgcgt cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct
     5101 gccgggtgat ttagccattc aggggcgata atcccttccg tttaaagagc aaacccctca
     5161 aacgaggggt tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca
     5221 actcgatgct gccgtcttcg ttttgctgtt cgagcatcac atcaaatccc cacaggcgat
     5281 gcacatgctt cagcacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatgt
     5341 aacgcagcgt cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct
     5401 ccagattact taagttatat tgcgacgata accggttacg gatctcccga taaccttctt
     5461 cattatgaat agcggaaatc tccagataat tatgccgatc atcatccagc acggtgaaga
     5521 agcggaaatc acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga
     5581 aatcacgcat cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc
     5641 agtatttgtc ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga
     5701 acccgagggc atacgggttg atgccgctgt accacgggct gttatagggg ggctggaaga
     5761 ccacattggt gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat
     5821 catacagatg gttaaggatg gtatagtgcc agaaggtcgc ccagccttcg ttcatcacct
     5881 gagtctgttt ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac
     5941 gctgccatga ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg
     6001 gttcggacgg atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg
     6061 tacgccagag catattgacc tgactttgta gatactcttc gcgacttttc tgccgggctt
     6121 tctcttcttg cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca
     6181 gcgcgtggca cgagtccaga agccgttcta cttcatcaac gccataacgt tcttcgcact
     6241 cggtaatata tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccaac
     6301 tgcggaataa gtaattgttt ttaaagaaag agttatgccc atagcaggcg tgtgccatca
     6361 ccagcgcctg catggtaatg gtgttctctt ccatcaggta agcgatacac gggttagagt
     6421 taatgacgat ttcataggcc agtccttgct gaccgtgctt atacagccgt tcagtctcga
     6481 taaacttttt accgaatgac cagtgcggat agttaattgg catgccgacg ctggagtagg
     6541 catccatcat ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt
     6601 agagtttcgc cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt
     6661 cgggtccatc gctcaaacgt gtggtgtcct tattcataga atcgatcgtc gccatacgcg
     6721 cacctcattg ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat agaacacctg
     6781 ttaaaaaccg cgtcgccgga gaattttttt ctttgcgatt tcttattatc agagtgccac
     6841 taatccgctt ctgaacggaa ttttatgctg gataaaaagg gcgttcagca ggagatacta
     6901 aagacgccat attgccgcag agtcagggag atgtgagcca gctcaccata aaaaagccgt
     6961 atgttgaata atattttcaa ctgagttatc aagatgtaat tagattatta ttcttttact
     7021 gtatctaccg ttatcggagt ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc
     7081 gttgccagcg cctggtactt aaatcaggca ggacatgagg tcaccgtcat tgatcgggag
     7141 ccgggggccg ccctggaaac cagcgccgcg aatgccgggc aaatctcccc cggatatgct
     7201 gcaccgtggg cggcaccagg tgtgccttta aaagcgatta aatggatgtt ccagcgccat
     7261 gcgccgctgg cggttcgtct cgacggtacg cagttccagt tgaaatggat gtggcaaatg
     7321 ttacgtaact gcgacaccag ccactacatg gaaaacaaag ggcggatggt gcgtctggcg
     7381 gaatacagcc gtgattgcct gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg
     7441 cgtcagggtg ggacgctgca actgttccgt accgaacaac agtatgaaaa tgcgacccgc
     7501 gatatcgccg tgctggaaga tgccggcgta ccgtatcagc tgctggaatc cagccgcctg
     7561 gcggaagtgg agcccgcgct ggcagaagta gcgcacaaac tgacgggtgg tctgcaactg
     7621 cccaatgatg aaaccggaga ctgccagctg tttacccaga atctggcgcg gatggcagag
     7681 caggcggggg ttaaattccg ctttaatacg cccgttgacc aactgctttg cgacggcgag
     7741 caaatctacg gcgtgaagtg tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg
     7801 tttggttctt actcgacggc gatgctcaaa ggcattgttg atattccggt gtatccgctg
     7861 aaaggctact cgctgactat tccaattgcg caggaagatg gtgcgccagt atccaccatt
     7921 cttgatgaaa cctacaaaat cgccattacc cgtttcgata accgcattcg tgttggcgga
     7981 atggcggaaa ttgttggttt taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa
     8041 atggtggttc gcgatctcta tccacgcggc ggtcatgtcg agcaggcgac tttctggacg
     8101 ggtttgcgcc cgatgacgcc agacggtacg ccggttgtcg ggcgtacacg ctttaaaaat
     8161 ctgtggctga ataccggtca cggcacgctc ggctggacga tggcttgcgg ttccggtcag
     8221 ttgttaagcg atctgctctc tggtcgcacg ccagcgatcc catatgagga tctaagcgta
     8281 gcgcgctaca gccgtggatt tacgccatca cgtccgggcc atttacatgg cgcacacagc
     8341 taaggaaacg agatgacccg tccgatacag gccagcctcg atctgcaggc attaaaacag
     8401 aatctgtcca ttgtccgcca ggccgcgccg cacgcgcgcg tctggtcggt ggtaaaagcg
     8461 aacgcttacg ggcatggtat tgagcgtatc tggagcgcgc tcggggccac cgatggcttt
     8521 gcattactta acctggaaga ggcaataacg ttacgtgagc gcggctggaa ggggccgatc
     8581 ctgatgctgg aaggattttt ccatgctcag gatctggaga tttatgacca gcaccgcctg
     8641 accacctgcg tacacagcaa ctggcagctc aaagcactgc aaaatgcgcg gctaaaagca
     8701 ccgttggata tttatcttaa agtgaacagt gggatgaatc ggttgggctt ccagcccgat
     8761 cgcgtgctta ccgtctggca gcagttgcgg gcaatggcga atgttggcga gatgaccctg
     8821 atgtcgcatt ttgccgaagc ggaacatcct gatggaattt ccagcgcgat ggcgcgtatt
     8881 gagcaggcgg cggaagggct ggagtgtcgg cgttcgttgt ccaattcggc ggcgactctg
     8941 tggcacccgg aagcgcattt tgactgggtt cggcctggca ttattttgta tggcgcttcg
     9001 ccgtccggtc agtggcgtga tatcgccaat accggattac gtccggtgat gacgctaagc
     9061 agtgagatta ttggtgtcca gacgctaaaa gcgggtgagc gtgtgggcta cggcggtcgc
     9121 tatactgcgc gcgatgaaca gcgaatcggc attgtcgccg cagggtacgc cgacggttat
     9181 ccgcgccacg cgcctaccgg tacccctgtt ttagtggacg gcgtgcgcac catgacggtg
     9241 gggaccgtct cgatggatat gctagcggtc gatttaacgc cttgcccgca ggcggggatt
     9301 ggtacgccgg ttgagctgtg gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc
     9361 ggaacggtgg gctatgagtt gatgtgcgcg ctggcgttac gcgtcccggt tgtgacagtg
     9421 taacttgttg taagccggat cggagacagc gtcttctggt tgcgaaaaaa tcatccatcc
     9481 ggctggtcag caactgtagt tgttaatgtg acagagccat tgcccatgat agtgtccatt
     9541 aaaagaatgg acactatttc cccggaacct gaactcaccg cacaggcgtt ctacataaaa
     9601 cgcttacgct tcattgttga ctcgaactcg acttcagata aatcacgctt tacccttgat
     9661 ggagcctgta catagatttg tgtaattgcc tgattttgat atgttcaatt caacatcaaa
     9721 tgaaggttaa attatggacg acaaacaatt gcaggctcag gctgcgttca gcaaagcatc
     9781 gcaaccggcg atagatgctt cattaaattt aagattcagc ttcctcttca gccacccgta
     9841 cgccaatctt caacacttca ttatcttctt tctcggccac cgtccagatc atcccggcaa
     9901 actctacttg gtcaccgaca accggtgccg cgcctaacaa ctgctggaca atttcaccca
     9961 gcgtttgctg cttatcacga tattctcgcc cgtcttccag gccatatatc agcgcaacat
    10021 cagcatattt ggcgctggct tcgagaatga agtcaccaaa gaagcgttga tccagcgcga
    10081 ccggcggcga ctggctgaac agtttaccga gcgccggaag atcgcgttcc cgaccaatta
    10141 cacacaacac atcgccttcg cgcagtcggg tgctgccggt aggatgaagc aactggttat
    10201 cacgaaacag tgccgcaata cgcgtctctt ttggcatatg caaatcgcgc agtgccgcgc
    10261 ccacgcacca tttatcggca ctcaattgat aaacaaactg ctcccacgga ttttccggat
    10321 gaatatccag gccaacgcgt gacaccggac gtcccactgg cggaaccacc actttggctt
    10381 ttttagccgc ccacgagagt gatgttccct gcaagagcag tgaaaccaga accacaaaga
    10441 aggcgacatt aaagaacaga cgtgcattct ccagccccgc catcatcggg aacacggcca
    10501 ggatgattgg caccgcgccg cgtaatccta cccagctgat aaacacgcgc tcacgcagat
    10561 tgaaaccgcg gaaggggagc aatccagcaa ataccgaaag gggacgggcg aagaatatca
    10621 tccatgccga caaaatgagc gccggaatgg caataggcag cagatcgctt gggttaacca
    10681 atagccccag caccaggaac atggcgattt gcgccagcca ggcgaggccg tcgaaatttt
    10741 gcaggatgcc gtagcggttg cgaatcggcc gattacccag cagaaaaccg cacagataaa
    10801 cagccagaat accgctgcct tccagcgccg tggttaaagc aaaaattaaa ataccgccgc
    10861 ttaatgccag caatggatat aatccggcag gcagggcgat gcgattaatc atttgcagca
    10921 gtaaataacc accgccaagt ccgatgacaa tcccgaggcc aaattgctgc agaatatcga
    10981 ccacgaacat ccaactgaca ctgctttcat gttgctggat catcgcaatc agggtaatcg
    11041 tcagaaagac cgccattgga tcattactgc cggattcaat ttccagcgtc gagccaacac
    11101 gttcgttaag ccccttacca cccagcagag aaaagaccgc tgcagcatcg gtagagccga
    11161 cgatagcgcc gattaataag ccttcaatca aatcaagatt aaacagccac gccgccatca
    11221 tgccggttaa accagaggtg ataagcacgc ccagcgtcgc cagcgacagt gccggtccta
    11281 acgccacacg aaaggaactg gcctgagtgc gcatcccgcc gtcgagcaaa ataatcgcca
    11341 gtgccaggtt acttaccatg taggcgaagg ggtaattatc aaacgggata ccgccgacgc
    11401 catcgactcc tgccagcatg ccgatcgcca gaaaaataac cagaatagga atgccaagac
    11461 gggaagaaaa tgaactaagt aatatactgc tggtgacaag gatggatcct aagatgaaaa
    11521 ggctaattat tgttgtggca tccaacggtc ggttactcct gattacgctg tctcttatat
    11581 aaaccctacc atattagcgg cagagacagc gttttactta gccctgaagc gtgatttttt
    11641 tacattttaa gaacaggatg accgctaatc gtcaactgag tgccttcccg ggtgttattc
    11701 agaatggcat gtgcgcccaa cggcagcgta accgtgcgtt gttcatgacc aaaatcgagg
    11761 ccggtaatga gtggaatcga caggcgggaa cgcaaaaacg cgtacactga ctccaggttg
    11821 taacctgcgt cataatcatt gggcgtgctg ccgctaaagc taccgagaat aatcgccttc
    11881 tgacgtggca aaattcccgc atgatagagc tgcaacagca tacgttcgac gcggaaaggg
    11941 tgctcgttaa tatcttccag taccaggata ccgttctcaa tttttggcat ccacggtgta
    12001 ccaatcagtg aaatcagcat cgcaagattg cctccccaca acgtgccttc ggtctgacat
    12061 gtcgggcctt ccccttgcca ttcaatggtg aaggttttat tgcgtaacgc cagccagaaa
    12121 tggtgctcgg taaaggcgtt cagttcatcc gcaccaaagt ttgctaccag catggggccg
    12181 ctaaaggtga tgacattgcc ttgcgccaga agaccgcact gaatggcggt aaaatcgcta
    12241 tgtccgcaaa tgagtaacgg gtcatgttgt tggcgagcca ccagtgcctg ccagtcaata
    12301 tctgccagta aacgactggc accgtaaccg ccgcgtacag ccagcacgat ggtgttgggg
    12361 gtagtcagtc tggcaaggga attgagatct tccaggcgct ccgtttccgt acccgcaaaa
    12421 cgctcacaac gacgggcaat aacctcgacg ttattgacct gatgcccctc gtcggtaagg
    12481 cgatggatac cacgcagcgc ggcgtgctgt ttaatgcagt aacccgatgg ggcaattaag
    12541 tgaaacagag acatggcaat tccttgctga caacagaaac gaaatgtata tcatgccgct
    12601 taggtgtgcc gttgtcacct caacggcgat tccaggctat aaggatagaa gaagtgaaat
    12661 tgagatggtt tgcctttttg attgtgttat tagcgggttg ttcatcaaag catgactata
    12721 cgaacccgcc gtggaacgcg aaagttccgg tgcaacgtgc gatgcagtgg atgccaataa
    12781 gccagaaagc cggtgcagcc tggggcgtcg atccacaatt gatcacggcg attatcgcta
    12841 tcgaatcggg tggtaatccg aacgcggtga gtaaatcgaa tgccattggt ttgatgcagt
    12901 taaaagcttc aacctccgga cgtgatgttt atcgccgtat gggctggagt ggtgagccga
    12961 cgaccagcga gctgaaaaat ccagagcgta atatttcaat gggggcggct tacctgaata
    13021 ttctggaaac cggcccgctg gcaggcattg aagatccgaa ggtactgcaa tatgcgctgg
    13081 tggtgtcata cgctaacggg gcaggtgcgc tgctacggac tttctcgtca gatcggaaaa
    13141 aggcgatcag caaaatcaac gatttggatg ctgacgagtt cctcgatcac gtagcgcgaa
    13201 atcatcctgc gccgcaggct ccgcgctata tctacaaact tgagcaggca ctggacgcga
    13261 tgtaaatcag tcgcgcactt tgtccgcttt ttctcgggct tctcgctcga gagagaaaat
    13321 aatccgttgt aattgccgct ccaccgtcgg gctgacgtta agaaaacgga agctcagacg
    13381 gggagtggtg atggtttcat tcttgccatc aatcactttg cgctcgctga tggagattaa
    13441 ctgggcgtca aagtgaaaaa caccccattg ccccatgttg acttcaatct gagcgaagcg
    13501 catgccttct tgtaattcgg caggctttgc tgtttccagt aatgcgccca tgccgcctaa
    13561 cgacaaatca tacaggcgga aacgtaacgt actgttatcc gccagtttgg tctggcaaaa
    13621 ataaggcgga tggagtgggg cggagatgcg gaaatatcgg cgtcgttgta caaaccataa
    13681 ggtgggaggc ggtacggtga taaatgccgg aagctgcaag tattcactct gctgtagttg
    13741 ttcaacagta aactcgactt tcgcaccctg agtttcggcg gtaatggtaa tgtgctgtgc
    13801 ctttagcacg gcgatgttgt cttcggcttg actgccgaaa tccagcacca gtttatccgg
    13861 ggttattgcc agtattttgc tgatcagctg cccgccattc caactgagac gcaaaggaat
    13921 tgcggctttg tgcaaatcgc gtaacacgcc caggacggct aacggatttt gtttcaggaa
    13981 ctgctcatgg taatgactca cgcggaaaaa ctcctgactg acaaactgtc tttggtttat
    14041 cggtcacagt taacaaaact taatacaaat gcgtgaatat tttttacatg ttgttcttaa
    14101 atcagccgac atacgcccag catcgatccc gcccttgcct atacttagag cgttgacgta
    14161 agcatttctt gcgtccgatt catcgaaacg agggcctgaa catgggaatt attgcctgga
    14221 ttatttttgg cctgatagcc ggcattatcg ccaagctaat catgccgggg cgtgatggtg
    14281 gtggattttt cctgacctgt attctcggga tagtcggtgc ggtggtcggc ggctggctgg
    14341 cgaccatgtt tggcattggc ggctccatca gtggttttaa tctgcacagc ttcctggtgg
    14401 cggtggtggg agctattctc gttctgggca tattccgcct cctgcgaaga gaataagatt
    14461 ttcataaggc ggatagcgat acagatgccg ctatccgctt tcacatcaga acgtatactc
    14521 gacacctgct ttacgggtga aaaaaatcaa tatagcactt cgatatccga taaagtcggt
    14581 tcccggtaac aacaacagaa tcctttttta attattgttt cgttgttttt ggtgtgattc
    14641 agaaaataaa tattttagac agtagccagt caggcgttaa aaatggggcg gatagtgagg
    14701 cggttttggc tgcgcttatt gaacatttag aattgatcaa tccttctggc agattaagcc
    14761 aaaatacccg ttcggcaatg cttcagttac gcgaagagga atggtctgaa ttcttttttt
    14821 ggttgctaaa ttctctggag tgtctggatt atgtgattat taatttaact ccagaaagca
    14881 aaaaaacgtt gatgagtgaa caccgtaaca atattcaggt agcaattgat gctttatata
    14941 gccaacgtcg gcgtaaatcg ccgggtgatg aaagtgaaac acttacccgt agaaatgacg
    15001 ctatcttcgg caaccacgta tggcaaacat ttgcgcaata cttcccgcca ggacttgaaa
    15061 aaccgtctgt gtagaaaaaa ccatagctat ggtgttatct gccgacaacg ccatccggag
    15121 aagccgaatg acgttgtcgg agtaagcgct ggttaaggtg tgggttgtgc ctctttggtt
    15181 gagggttgcg tcgttgctga cttaacggtc ggacgcgtcg ccggaacatt gtcacacggt
    15241 tgctctttcg ggcagatcaa atccagcatt ttcagcgtca cgccattggt ccagccaaag
    15301 ccatcctgta atggatattc gccaccgccg ccccccgttc cggtggtgct gacatcatat
    15361 ttttccacca gctttttctc ccggtcatag gtgtgctgaa cattggtcag gaagtgccag
    15421 ctaatgtcca tcgccacctc tttttgcccg tagttttgta atccttctgt cgcgacccac
    15481 tgtaacggtg cccagccatt tggcgcatcc cattgttgcc cacttttcac cgacgtggtg
    15541 ttcaggccgc cgggttgcag cagatgtgtt ttcgtcgccg tcgccatttt gttggcgcga
    15601 tctttcgctg ccgcattgac gtacagcggg aacagggcgg ccgcggttaa ctgattgcgc
    15661 actttatgac ttttcaggtc gtaatcggca taccagcctt gttgatcgtt ccacaggtat
    15721 ttttcgatcc ctttttgacg ggcatttgcc agcgtttcgt actggtttgc catcgcgtta
    15781 tctccggcag ctttgctggc gcgggcgagg attttttcca ttttaaacat caggctgttc
    15841 agatcgaccg gtacgatgct ggtggtgcgt aaggtattta actgctgcgg gttgtccatc
    15901 cagcgcgagc tgaaatccca gccagacgca gcggcagagc gcaggtcgcg gtaaatttca
    15961 gtggcaggtc gattcggatt gcttttggcg gtggcaatat cttccaccca tgactctggt
    16021 cgtggcgtat cgcgatcgtc ccagtagcgg ttgagaaggg taccatcctg aagtttgaca
    16081 acgcgttttt cctgttgtcc ggcttgcagg ttttcaacac cgtccatcca gtaagcatat
    16141 tctttttgca tttgcggcag gtattgcttc aacgcggcat cgccttcatg ctgcgccagt
    16201 aactctacca tcagggcaaa gaagggcggt tgcgagcggc ttaaatagta actgcggttg
    16261 ccgttgggaa tatgaccgta agtgtctatt tcatgagcaa aattggccac catatccgcg
    16321 actttatccc agtgaccgct ttcggcaagt cctaacatgg tgaagtaact gtcccagtaa
    16381 tatacctcgc gaaaacgtcc gcccggcacg acataaggtt ccggcagcgg taacagagaa
    16441 tcccattttt ctgtgttttc ggtagaacgc gttaataccg gccaaagtcc gtcaatatgt
    16501 tcgcgcagtg actgcccctc tggcggaaca tatttctcgc cttctttcgg cagggtgaaa
    16561 ttgacgttaa cgaaatggcg cagatcaaat ccgctctggt tttgctgcat ccgataatca
    16621 gcaaggatca tcagcggatc gctgttcggc acggcatcgg caaaggtttt ttggtccgga
    16681 aaaagtttgg cgttttgcac atcattaaac agcggcccta ataaaatatc aggcggctgt
    16741 ggtgttaccg gtgtttcttc tgcctgcacc gatagcgcag cgaaacacaa aaagatacag
    16801 gctggaatta acgccatttt ttgcgggcga gaaggtgcgg gggatttcat caatcattct
    16861 cctttggcga aaccgaataa aacggtattc aagtatcaga aaaccttagt tcaggatcgc
    16921 actagctgca tgatcgaaat ctcattctgc gaactgtcag gcattttaga aaagggaatt
    16981 aaagggataa ataacggcga aatagagtga tcaaataacg tctgtttatt agccagccag
    17041 cggcgttggg ttcgtgatta gcgaagaaaa atcatcataa attggtgaaa aatataacag
    17101 gtagttaaaa accattagtg ctgagtaaat tgccggatga catcagaacg atgccatccg
    17161 aacagtggct taaccctgac ggttgaaacg ttgcgtttta acgtccagcg ttagcgtttc
    17221 ttctggttgt atcgcataca gtttctcacc ctgctggcaa atccagccaa tccccagctc
    17281 acgggcgatt agggcgctgt gggataccgg acttccggcg ctaaggcaga taccttttac
    17341 aaccgccgga tccagttgca gtactgtgga aggataaatg ttctccgcca gtagaatagt
    17401 tggcgagtta aactgcggga gttcttcttt cgtttgggtc aggtggacca gggtgcgatg
    17461 cagaagatcg tccacatcaa tatagcgagc ttgtagatat tcatcatcca gttgctggta
    17521 ttgctggcta agttctttaa gaacttgctg ccaggcatat tctgccgtgc aatgttcgtg
    17581 ctgaaggagt tcgcttgccg ccgccagcag ttccggatca tctaacagtg tatggtgacc
    17641 agaaaagatt gcggcaatat cgtcaagccc gctggcttct gctttcgctg ttaacgtcat
    17701 cagatctaat aacgtgaagt caatggcctg gcgtaatcgt tcttgttcct cttccacggt
    17761 cagggttgat tttgcctgta ccgtacataa aaccggttga taataaaagg ctttacccga
    17821 aacaggcgga acgggacgca gagtaggtgg aacaacttcc tccgtttcac caaagttatc
    17881 ttcagccagc tgacggaaag cgatcagtgc ctcttcagct tctggccctt tcgcaatcag
    17941 gcgcagcgta tcgttatagc gaacttgtag taacgcaatc tggttaatac tctctggtgt
    18001 gacgcatttg ccgttttttt ccagcaacat atcggcatta aatgtcgata aggtataaac
    18061 cagccgggag gccggacgta catgcaggcc gttacggttt tttatgacca ccgccagaga
    18121 acgggcttct tcatcgtacg caggacatgt gtcagagatt ttagtgtcgg aggacggtaa
    18181 acccagttgt tcacgtttgg cttccagcgc atgcatagcg tcaaagataa ctttgtcgat
    18241 atccgctccc gaggccgcgc tgaccgttgc tgccagtgta ccttcgacca acggcgcagc
    18301 acacaaacgt acttttgcgg cgatcttggg agccagcaat tccagcgcag tttcagcact
    18361 caataatgcg ctacccatat ccatcatgac cagcacatgg tcggcatcag caacagattc
    18421 gatggcctcc atcactttga cggcatcggt accaatagga ttttgtggat cgtcaattcc
    18481 cgcggcaatg gcgattttac aactatcact cattaacatc tgacgggcta attcaccgac
    18541 accttctccc agtcggctgc tatgtgaaac tatgaccagg tttaccatca ccaattcctt
    18601 actcttttgc ggctaacgcc aacatttgca tcataaacat caccgaggtc gcgccgggat
    18661 cctggtgacc aatactgcgt tcaccgagat aactggcgcg gcctttgcgg gcttgcatcg
    18721 taatcgtact ttgtgcagcg gattcggcga ttctactggc agcttcgagc gccaccggaa
    18781 cagagagatt ttgctcgctg gactgacgta acgattccac caccggcacc cacacatcac
    18841 acatggtttt atcgccaggt tcggctttcc cgcgactgat tacgccgtcc acgccatcgc
    18901 ggaacatctg ataaagctct tccagtgtca ggctttgccg tgcctgggtc gcctgtgcgg
    18961 cgcggataaa gaaggtaccg aacagcggac cactggcacc accgacgctg gaaagcagcg
    19021 tcataccggt attcttgaga atgaaaccga tatctttatc tgcgatagca gggagttttt
    19081 ccaccacttt gctaaagcct cggttcatat ttagcccgtg gtcagcgtcg ccaatttcgc
    19141 gatccagtcc ggtaagatac tcgctctcgg tgctgaaaat atcgccacaa cgagtgagcc
    19201 agttaacaat ttgagttctg ctcagtgaca ttgctttctc cttatttacc ccagttaagg
    19261 gccggggtgt ggaccggggc gtcccagagt gccagcgttt cgtcatcaac tttcagtaag
    19321 gtgattgaga aaccggtcat atccagtgag gtgcagtacg cgccaattaa attacgttcg
    19381 atagtcaatc ccgcttgctg gcaacgtgtg gtcaggcggt tatagacgcc gtacagctca
    19441 gaaagcggag ttgcgccaag attgttaacc agcgcaatca cccgatcgcc agactggagc
    19501 ggttgtttgg tttgttgttc ttcctgccaa ctgccttgtt gataatccca gaaacgcaaa
    19561 gtgcgatggt atgagccatt ttccagcagg gtgtcgaaca tttcatcgac ggtttgatca
    19621 agggaagaga aggagcggcg gtcaataccc ggctcaccat gaatgccgac gccaaactcc
    19681 atctcattat ccgccagggt aaaagaaggt ttgcccgcgg caggaacggt acaggcaccg
    19741 agagcgatac ctattgagtg gccttgatta ttcaacttac gccccagttc cgcacaggcg
    19801 tccagtgagt cgccacgctc cgccgctgcg cctacgagtt tttcaattaa tacggtgttg
    19861 gcaacgccgc gtcgcccggc ggtataaaga ctgtctttta ccgcaacgtc gtcatcaatg
    19921 accacagtgg tcacttttac gccgctatcg tgcagtaact cggtcgctgt ttcaaagtta
    19981 agaatatcgc cggtgtaatt tttgataatc aacagtacac cttcgccgcc atcaatttgc
    20041 atggagcatt caaagatttt atcgggcgtc ggtgaggtga aaatttcgcc cggacaggcc
    20101 cccgaaagca tcccctgacc gatataacca cagtgcatcg gctcgtgtcc gctgccgcca
    20161 cccgacagca gggcgacttt tcctgcaaca ggggcatcag ctcgggtgac atacaccgga
    20221 tcttgatgca gtgtcagcga tggatgcgct ttcgccagtc ctgccagttg ttcgtccagt
    20281 acgtcttgca catcattgat caattttttc attattttgc tccagcaatt acggtagggc
    20341 atggatgatg ttcaacgaca cggcgaccac tgactgccga tgaatccatt gtgcatcagg
    20401 caagggaaaa gaaaattagc ccaaattatg tttcatagtg aaacatatgc tttaatgaat
    20461 gttccatatt gaaactttta cgtgtattaa tacttaaaat tgcgagccgg aacacctttt
    20521 gtcataaggg atgcgggata tgagtggcgc ttttaacaac gatggtcggg gcatatctcc
    20581 cttaattgca acctcctggg agcgatgcaa taagctgatg aaacgggaga catggaacgt
    20641 accacatcag gcccagggcg tgacatttgc ttctatttat cggcgtaaga aagcgatgct
    20701 gacgctcggg caggctgcgc tggaagatgc ctgggaatat atggcaccgc gagagtgtgc
    20761 gctgtttatc ctcgatgaaa ccgcctgcat tctcagccgt aatggcgatc cgcaaacctt
    20821 gcagcagcta agtgcactgg gattcaatga cggcacgtat tgcgccgagg gaattattgg
    20881 tacttgtgcg ctatcgttag cggctatctc tggtcaggcc gtgaaaacga tggccgatca
    20941 acatttcaaa caggcactct ggaactgggc cttttgtgca acgccgttgt ttgacagcaa
    21001 gggccgattg acgggaacaa tagcgctggc gtgtccggtt gagcaaacta ccgcagctga
    21061 tttgccgttg acgttggcaa tcgcccgcga ggtcggaaat ttactgctga cggacagttt
    21121 gctcgctgaa actaaccgtc atttaaatca acttaatgcc ctgttagaaa gtatggatga
    21181 tggcgtgatt agctgggacg agcagggtaa tttgcaattt attaatgccc aggcggcgcg
    21241 ggtcttgcgc cttgacgcga cggcaagtca gggaagggca atcactgaac tcttaacgtt
    21301 acccgccgta ttgcaacaag caataaaaca ggcacatccg ctcaaacacg tagaagcaac
    21361 ctttgaaagt cagcatcagt ttattgatgc ggtgataacc cttaaaccga taatagaaac
    21421 gcagggaacc agctttattt tgttgctcca tcctgtggaa cagatgcggc agttgatgac
    21481 cagtcaatta ggaaaagtca gccatacctt cgctcatatg ccacaggacg atccgcaaac
    21541 ccgccgcttg attcattttg gtcgccaggc ggcgcgcagt agctttcctg tcctgctttg
    21601 tggagaagag ggcgtgggaa aggcactgct aagtcaggca attcataatg aaagcgagcg
    21661 tgctgcaggt ccttatatcg ccgtcaattg tgagttatat ggtgatgcgg cgctggcgga
    21721 agaatttatt ggtggcgatc gcacggacaa tgaaaatggc cgtctgagtc ggctggaact
    21781 ggcacacggc ggcacgctgt ttcttgaaaa gattgaatat ctggcggtgg agttacagtc
    21841 tgctttgctt caggttatca agcagggtgt tatcacgcga ctggatgcgc ggcgtttaat
    21901 accagttgat gtcaaagtga ttgccacaac gaccgcggac ctcgcaatgc tggtggaaca
    21961 aaatcgtttt agtcgccagc tgtattacgc gttgcatgca tttgaaatta ccatcccgcc
    22021 tctgcgtatg cggcgtggca gcattccggc gctggtgaat aacaaattac gcagtcttga
    22081 aaaacgcttc tctacgcggc tgaaaattga tgacgatgcc ctcgctcgcc tggtttcctg
    22141 tgcatggcct ggcaacgatt ttgaacttta cagcgtcatc gagaatcttg ctctgagtag
    22201 tgataatggg cgcattcgcg tcagtgattt gccggaacat ctgtttaccg agcaggcaac
    22261 agatgatgtc agcgccacac gcctttccac cagtctgtca tttgcggaag ttgaaaaaga
    22321 ggcaattatt aacgcagccc aggtcacagg cggtcgcatt caggaaatgt cggctttact
    22381 tgggatcggc cgcactacgc tgtggcggaa aatgaagcaa catggcattg atgcagggca
    22441 gtttaagcgc cgggtatgaa agacagagac gatttctgat acatcagagt gatctatatt
    22501 tcattccggc gcacgctaac atttttcagc atcgtttaag ggctggtcta tcccgcactt
    22561 aaaagctgaa gcgatatcct ccgttgactt gcttctgatc aaagaggtta ctctgcgtgt
    22621 aatccgcttc gaggtagaat gtgtgctgtt tgttatactg tgcactgact cccacgccgt
    22681 tattccagcc attacctttg aaactgtact tctcccggga gttgttcaac agatagtctg
    22741 tatcccctga aaactcgcgg atagctccag tcttcacata catattcagc tggctgttgc
    22801 ctgcggtgat gtcatatccc agtatcatgc tggcacgccc cagcagcgat tcgtagtgat
    22861 tcagatgtat attgagacca ttactcgcct tcatagccat ctcattctgg tggctgtatg
    22921 tgagctgggt ttgcggctct atatagaacc cataaccagt aggggtcagg ttgaacctct
    22981 gcccggcttc cagggagatg ctcaatccat tcgcagtgcc gttggcgtta acgccgttgt
    23041 tctgactgtc cagtacgtga aaactatttt tctggcgcga tgcttttaca acgagatcgc
    23101 tgtaaaaacc gttgtgtgcc atgtaactgg catacattcc catgtagtct gaacgtgcgg
    23161 taccgtcgcc tccgctatag tccggcgatg catgtgttga gccaatatac agaccgacat
    23221 acaacggcat tacatcagag agacgtttat ccccaccaaa ctggataccg ctgtaaccca
    23281 tgtcaaagcc gctcagtttg ccactggcga aggagtccag gcttccccca taactgcgca
    23341 gccagatatt accgtcttta ctctgatttc gcaggtcacc cattcgttgc atcagcgtac
    23401 ggttttcaac atagtttaat aagtaaccga cattgagata attgccgcca gcatctgcgg
    23461 tcgtggtggg tttaggtgtg ggagtggggt taggcgtagg atcgggttca gacgtaggat
    23521 cggggttgac tatgggaggc tgagcgggag ctggtgtggg ttcaggatta ggagtcggtt
    23581 ccggaactgt cccggaagcg taaagctccc agttagtgcc atttttacgc acatcgtaca
    23641 gatatccccc caactcaacc tgagaagacg cgctgaacga ggccgcgccg tcagtggttt
    23701 ttaccactgt cagaacttca tttcccgttg tggcctcgct gccctggttg cggatagcca
    23761 atacgtgatt accagcacta ctcccgctga tattcaataa atccccttta ttattaacgc
    23821 cattaccctc gccaacaaca tcagcacgca taataaaggt actgttaccg ctcaggttct
    23881 ctacgtttaa tgtggcaaat gtgccggcag ttgacccgtg gctggcaaaa tcgacagttg
    23941 aatggctcag cgccagcgtg tcgagattag aattacttgt tacgttccag acgctgttat
    24001 tcattgcaac gtccagtttt ccgccattga cattatcgct gagggaggaa cctgtccaaa
    24061 ccgacccagg gtgcatatcc agattgatta gcccaccttt ggaaagaacg ctaccgttga
    24121 taagcatacg acccgaggcg ttgatgcggc tggcggcata accatcgtca tgttgcgttg
    24181 caatggccat ctggtcgggt gtactcatat caatgaccag atcgctcgtg aggtcgatct
    24241 ggctgttggt catggcataa attcccctgg ctcctgccgc gccggtgata gccaaactgt
    24301 ctccggttat tctaccgccg ctgagcgccc acaaacccag cgccagactg ccattacgat
    24361 caactgtaat atcggtattt tgcatgttga caacagccgt tgccgtctgg gccgaggcac
    24421 cataagaacc gccggaaaag atgctgtttc gttgcgccgc cgtgccagaa aaattgattg
    24481 tcgcgtctga accactggtg acgaggccac cgccctgcgc ggaagaaata tggctatctg
    24541 caccgatagt ggttgtacca ccacgaactt cgacgccatt ggccgcggct ccagttacat
    24601 caacagtgag tgcattcgcg ctaacctggc caaagctcca gaggccgtgt gcattatcgc
    24661 cattggtttt aatggaactg tttgttccga gatcgacaac agagtttttc tgtacgttta
    24721 tccccatggc gctgtagccc tgaacatcga ttgtcaggtc tgtagccgta aaacgcgcag
    24781 caccattggc gttattgccg ttgagaccac cgatataaac acctgtactt ccatcggtcg
    24841 tgattttact tccgcttcca agatcgacac tggtgccata gtcattgatg gttaggccta
    24901 taccgttcga gttttcaatg gtgaattgag tggctgttag tgttgagcta tgcccaataa
    24961 tgatgccgtc atcgttactc ttaatggtgc tgcctgtgcc taagtcagca tgggtatagt
    25021 caccaattaa gttaatgccg atggcagagg tttgaccaac aacatctact gtgagtcggt
    25081 tagctgtcag ggagctgttt ttgccctgaa taatgattcc ttttgcatat ccagaggcgt
    25141 cgttgacatt gacggtgaca tcattaccca ggttgatatt gccaggcgtg tttcctgctg
    25201 gggtcattac accatagagg tttcctgatg gatcggcggt atctcctgta attctgtcac
    25261 catcattaat agtaatattg gtgccatatt gacttcctgt atcagcagct atcagcaggg
    25321 gggaaaagat aattgcattc aaaccaatag cgccaaattt tatcagttga attgagggcg
    25381 aacggatttt taattcagcg agacgatctg ctttggtatt cccattgtgt tgtttgatgc
    25441 ccataccact cctat
//
LOCUS       DAZFRN010000067        24970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFRN010000067 DAZFRN010000000
VERSION     DAZFRN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.067"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            446..1549
                     /gene="ompC"
                     /locus_tag="ACHKUB_002591"
     CDS             446..1549
                     /gene="ompC"
                     /locus_tag="ACHKUB_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6655594.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1661..2716
                     /gene="apbE"
                     /locus_tag="ACHKUB_002592"
     CDS             1661..2716
                     /gene="apbE"
                     /locus_tag="ACHKUB_002592"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO6655595.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2790..3854
                     /gene="ada"
                     /locus_tag="ACHKUB_002593"
     CDS             2790..3854
                     /gene="ada"
                     /locus_tag="ACHKUB_002593"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO6655596.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3854..4504
                     /gene="alkB"
                     /locus_tag="ACHKUB_002594"
     CDS             3854..4504
                     /gene="alkB"
                     /locus_tag="ACHKUB_002594"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO6655597.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4580..6223
                     /gene="yojI"
                     /locus_tag="ACHKUB_002595"
     CDS             4580..6223
                     /gene="yojI"
                     /locus_tag="ACHKUB_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO6655598.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLIQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6441..8087
                     /gene="mqo"
                     /locus_tag="ACHKUB_002596"
     CDS             6441..8087
                     /gene="mqo"
                     /locus_tag="ACHKUB_002596"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO6655599.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQFKPQPVQKEVADIAL"
     gene            complement(8236..8745)
                     /gene="eco"
                     /locus_tag="ACHKUB_002597"
     CDS             complement(8236..8745)
                     /gene="eco"
                     /locus_tag="ACHKUB_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO6655600.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(8859..9023)
                     /locus_tag="ACHKUB_002598"
     CDS             complement(8859..9023)
                     /locus_tag="ACHKUB_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655601.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNCYKHIYIAINSPEI
                     FLLVCSLTRI"
     gene            9132..9626
                     /gene="napF"
                     /locus_tag="ACHKUB_002599"
     CDS             9132..9626
                     /gene="napF"
                     /locus_tag="ACHKUB_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO6655602.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAMIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9616..9879
                     /gene="napD"
                     /locus_tag="ACHKUB_002600"
     CDS             9616..9879
                     /gene="napD"
                     /locus_tag="ACHKUB_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO6655603.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9876..12362
                     /gene="napA"
                     /locus_tag="ACHKUB_002601"
     CDS             9876..12362
                     /gene="napA"
                     /locus_tag="ACHKUB_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO6655604.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12369..13064
                     /gene="napG"
                     /locus_tag="ACHKUB_002602"
     CDS             12369..13064
                     /gene="napG"
                     /locus_tag="ACHKUB_002602"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO6655605.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13051..13914
                     /gene="napH"
                     /locus_tag="ACHKUB_002603"
     CDS             13051..13914
                     /gene="napH"
                     /locus_tag="ACHKUB_002603"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO6655606.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13911..14360
                     /gene="napB"
                     /locus_tag="ACHKUB_002604"
     CDS             13911..14360
                     /gene="napB"
                     /locus_tag="ACHKUB_002604"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO6655607.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14370..14972
                     /gene="napC"
                     /locus_tag="ACHKUB_002605"
     CDS             14370..14972
                     /gene="napC"
                     /locus_tag="ACHKUB_002605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO6655608.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14985..15608
                     /gene="ccmA"
                     /locus_tag="ACHKUB_002606"
     CDS             14985..15608
                     /gene="ccmA"
                     /locus_tag="ACHKUB_002606"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO6655609.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15605..16267
                     /gene="ccmB"
                     /locus_tag="ACHKUB_002607"
     CDS             15605..16267
                     /gene="ccmB"
                     /locus_tag="ACHKUB_002607"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO6655610.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLACTATLSPFATAAALRIS
                     IQ"
     gene            16309..17046
                     /gene="ccmC"
                     /locus_tag="ACHKUB_002608"
     CDS             16309..17046
                     /gene="ccmC"
                     /locus_tag="ACHKUB_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO6655611.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            17043..17252
                     /gene="ccmD"
                     /locus_tag="ACHKUB_002609"
     CDS             17043..17252
                     /gene="ccmD"
                     /locus_tag="ACHKUB_002609"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO6655612.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17249..17728
                     /gene="ccmE"
                     /locus_tag="ACHKUB_002610"
     CDS             17249..17728
                     /gene="ccmE"
                     /locus_tag="ACHKUB_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO6655613.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17725..19668
                     /gene="ccmF"
                     /locus_tag="ACHKUB_002611"
     CDS             17725..19668
                     /gene="ccmF"
                     /locus_tag="ACHKUB_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO6655614.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19665..20222
                     /gene="dsbE"
                     /locus_tag="ACHKUB_002612"
     CDS             19665..20222
                     /gene="dsbE"
                     /locus_tag="ACHKUB_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO6655615.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20219..21271
                     /gene="ccmH"
                     /locus_tag="ACHKUB_002613"
     CDS             20219..21271
                     /gene="ccmH"
                     /locus_tag="ACHKUB_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO6655616.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21306..21953)
                     /gene="narP"
                     /locus_tag="ACHKUB_002614"
     CDS             complement(21306..21953)
                     /gene="narP"
                     /locus_tag="ACHKUB_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO6655617.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22273..24864
                     /gene="yejO"
                     /locus_tag="ACHKUB_002615"
                     /pseudo
     CDS             22273..24864
                     /gene="yejO"
                     /locus_tag="ACHKUB_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         5736 a         6134 c         7070 g         6030 t
ORIGIN      
        1 aagtgtgtaa agaagggtaa aaaaaaccgg atgcgaggca tccggttgaa ataggggtaa
       61 acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag cgggagttat
      121 tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact taagaataag
      181 ttattgattt taaccttgaa ttattattgc ttgatgttac gtgcttattt cgccattccg
      241 caataatctt aaaaagttcc cttgcattta cattttgaaa catctatagc gataaatgaa
      301 acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt tggggagaat
      361 ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa aagcaaataa
      421 aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc tggtcccagc
      481 tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg gcaacaaatt
      541 agatctgtac ggtaaagtag acggcctgca ctatttctct gacaacaaag atgtagatgg
      601 cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg accagctgac
      661 cggttacggc cagtgggaat atcagattca gggcaacagc gctgaaaacg aaaacaactc
      721 ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt tcgactacgg
      781 tcgtaactac ggcgttgttt acgacgtaac ttcctggacc gacgtactgc cagaattcgg
      841 tggtgacacc tacggttctg acaacttcat gcagcagcgt ggtaacggct tcgcgaccta
      901 ccgtaacact gacttcttcg gtctggttga cggcctgaac tttgctgttc agtaccaggg
      961 taaaaacggt agcgtaagcg gcgaaggcat gaccaacaac ggtcgtggtg ctctgcgtca
     1021 gaacggcgac ggcgttggcg gatctatcac ttatgattac gaaggcttcg gtatcggtgg
     1081 tgcgatctcc agctccaaac gtactgatga tcagaacagc ccgctgtaca tcggtaacgg
     1141 cgaccgtgct gaaacctaca ccggtggtct gaaatacgac gctaacaaca tctacctggc
     1201 tgctcagtac acccagacct acaacgcaac tcgcgtaggt tccctgggtt gggcgaacaa
     1261 agcacagaac ttcgaagctg ttgctcagta ccagttcgac ttcggtctgc gtccgtctgt
     1321 agcatacctg cagtctaaag gtaaaaacct gggtgtcatc aatggtcgta actacgacga
     1381 cgaagatatc ctgaaatatg ttgatgttgg cgcgacctac tacttcaaca aaaacatgtc
     1441 cacctacgtt gactacaaaa tcaacctgct ggacgacaac cagttcactc gtgacgctgg
     1501 catcaacact gataacatcg tagctctggg tctggtttac cagttctaat ctcgattgat
     1561 atcgaacaaa gggcctgcgg gccctttttt cattgttttc agcgtacaaa ctcatttttt
     1621 tggtgtactc ttgcgaccgt tcgcatgagg ataatcacgt atggaaataa gctttacccg
     1681 cgtggcactg ctggctgccg cgctcttctt tgttggttgc gatcaaaaac cacaacccgc
     1741 caaaacccac gctactgaag ttaccgttct tgaaggcaaa accatgggta ccttctggcg
     1801 tgccagcatc ccgggcattg atgccaaacg cagtgccgaa cttaaagaaa agattcagac
     1861 ccagctggac gccgacgatc agctgctttc gacctataaa aaagattccg cgctgatgcg
     1921 ctttaacgac tcgcaaagtt tgtcgccgtg gccagtaagt gaagcgatgg ccgaaatcgt
     1981 cactacctcg ctgcgcattg gcgcgaagac cgatggtgcg atggatatta ccgtcgggcc
     2041 gctggtgaat ctgtggggct ttggcccgga acaacagccg gttcaaattc cgagccagga
     2101 acagatcgat gcgatgaaag ccaaaaccgg cttacagcac ctgacggtca tcaatcagtc
     2161 gcatcagcaa tatctgcaaa aagacctgcc ggatttatat atcgatctct ccaccgtcgg
     2221 tgaaggttat gcggcggatc acctggcacg cttgatggag caggaaggga tttcccgcta
     2281 tctggtgtcg gtgggcggcg cgctgaacag ccgtggtatg aacggtgaag gccagccgtg
     2341 gcgggtagcg attcaaaaac caaccgataa agaaaacgcg gttcaggctg tggtggatat
     2401 caacggtcat gggattagca cctctggcag ctaccgtaac tattacgaac tggacggcaa
     2461 acgtctttct catgttatcg atccgcaaac cgggcgtccc atcgaacaca atctggtatc
     2521 cgtgacggtg attgctccga cggcgctgga agccgatgcc tgggatactg gcttgatggt
     2581 actcgggccg gagaaagcca aagaggttgt tcgccgggaa gggctggcgg tttatatgat
     2641 caccaaagaa ggcgatagct ttaaaacctg gatgtcacca cagtttaaaa gcttccttgt
     2701 cagcgaaaaa aattaaagcg caagattgct ggtttttgcg tgatggtgac cgggcagact
     2761 aaaggctatc cttaaccagg gagctgatta tgaaaaacgc cacatgctta actgacgatc
     2821 aacgctggca atctgtctta gcccgcgacc cgaatgccga cagcgaattc gttttcgccg
     2881 tgcgcaccac aggcatcttt tgccgtccgt cttgccgcgc cagacatgcc ttgcgggaaa
     2941 atgtctcctt ctacgcaaat gcctgcgagg cactcgccgc cggatttcgc ccctgcaaac
     3001 gctgccaacc tgataaagcc aatgcccagc aacatcggtt ggataaaatc acccacgcgt
     3061 gtcgactgct ggaacaggaa acgcctgtaa cgctggaagc cttagccgac caggtggcga
     3121 tgagtccatt tcatctacat cggttgttta aagcgactac cggaatgacg cctaaagcct
     3181 ggcaacaggc ctggcgcgct cgccgtttgc gcgaatcgct ggcgaaaggg gagagcgtga
     3241 cgacgtctat tcttaacgcc ggattccccg acagcagcag ttactatcgc aaagctgacg
     3301 aaacgctggg catgacggct aaacaattcc gtcacggtgg cgaaaatctg gcggtgcgtt
     3361 acgcgctggc tgattgtgag ctgggtcgtt gcctggtggc agaaagcgag cgggggattt
     3421 gcgcgatatt gctgggcgat gatgacgcga cactaatcag cgagttgcag cagatgtttc
     3481 ccgctgccga caacgcgcct gccgatctga tgtttcagca acatgtgcgt gaagtgatcg
     3541 ccagcctcaa tcaacgcgat acgccgctga cgttaccgct ggacattcgc ggcactgctt
     3601 ttcagcaaca agtctggcag gcactgcgca cgataccttg cggtgaaacc gtcagttatc
     3661 agcaactggc taacgccatc ggcaaaccga aagcggtacg ggccgttgcc agcgcctgtg
     3721 ccgccaacaa gctggctatc ataataccct gtcatcgggt ggtccgtggt gatggcacac
     3781 tttccggtta ccgctggggc gtgtcgcgta aagcgcaact gctgcgccgc gaagctgaaa
     3841 atgaggagag gtaatgttgg atctgtttgc cgatgctgaa ccgtggcaag agccactggc
     3901 ggctggagcg gtaattttgc accgttttgc ttttaacgct gcggagcaac tgatccgcga
     3961 tattaatgac gttgccagcc agtcgccgtt tcgccagatg gtcacccccg ggggatatac
     4021 catgtcggtg gcgatgacca actgtgggca tctgggctgg acgacccatc ggcaaggtta
     4081 tctctattcg cccattgatc cgcaaacaaa taaaccgtgg cccgccatgc cacaaagctt
     4141 tcatgattta tgtcaacgtg cggctacggc ggcgggctat ccggatttcc agccagatgc
     4201 ttgtcttatc aaccgctacg ctcctggcgc gaaactgtcg ctgcatcagg ataaagacga
     4261 accggatctg cgcgcgccga ttgtttctgt ttctcttggc ttacccgcga tttttcaatt
     4321 tggtggcctg aaacgtaatg atccgctcaa acgtttgttg ttggaacatg gcgatgtggt
     4381 ggtatggggc ggtgaatcgc ggctgtttta tcacggcatt caaccgctga aagcggggtt
     4441 tcatccactc accggcgact gccgctacaa cctgacattc cgtcaggcag gtaaaaaaga
     4501 ataaaaataa gaattattat tgctgtgcgc gcgaagatgt ttaaactgcg ggctgtaatt
     4561 cattgtccgg gttttctgca tggaacttct tgtacttgtc tggcggcagt atcgctggcc
     4621 atttatcagt gtgatggcgc taagcctcgc cagtgcggca ttaggcattg gcttaattgc
     4681 ttttatcaat cagcgcctta tcgaaacggc ggataccagt ctgctggtgt tgccggagtt
     4741 tctgggatta ttgctgctgt tgatggcagt cactctcgga tcgcaactgg cgctcaccac
     4801 tttggggcat cacttcgttt accgactgcg tagtgaattt atcaagcgga ttctggatac
     4861 ccacgtcgag cgcattgaac aactcggtag cgcctcgttg ctggcgggat taaccagcga
     4921 tgtgcgcaat atcaccattg cttttgtgcg tcttccggaa ctggtgcagg ggatcattct
     4981 cactatcggt tccgcggcgt atctgtggat gctgtcgggc aaaatgttgc tggtaacggc
     5041 tatctggatg gcgatcacca tctggggtgg ttttgtactg gtggcgcggg tgtacaaaca
     5101 tatggcgacc ctgcgtgaaa ccgaagacaa gctgtacacg gattttcaaa ccgtactcga
     5161 agggcgcaaa gagctgactc tgaaccggga acgcgccgag tatgtgttta acaacctcta
     5221 catccccgat gcgcaagagt atcgccatca tattattcgc gcagacacct tccatcttag
     5281 cgccgtgaac tggtcaaaca tcatgatgct gggcgctatc ggcctggtgt tctggatggc
     5341 gaacagtctc ggttgggctg ataccaacgt tgctgcgacc tattcgttga cgctgttatt
     5401 cctgcgtacg ccgctgcttt cggcggttgg cgcattgccg acgctgctga cggcgcaggt
     5461 ggcgtttaac aagctgaaca aattcgcgct cgcgcctttc aaagctgagt ttccgcgccc
     5521 gcaggctttt cccaactggc aaacgctgga gctgcgtaac gtgacgttta gctatcagga
     5581 taacgcgttt tccgttgggc cgattaacct caccattaaa cgtggcgagc tgctgtttct
     5641 gattggtggc aacggtagtg gaaaatcgac gctggcgatg ttgctgacgg gcttgtacca
     5701 gccacaaagc ggcgaaatct tgctggatgg caaacctgtc agtggcgaac aaccggaaga
     5761 ttatcgcaaa ctgttttcgg cagtgtttac cgatgtctgg ctgtttgatc aactgctggg
     5821 gccggagggt aaacccgcta acccgcaact ggttgagaag tggctggcgc agctgaaaat
     5881 ggctcataag cttgagttaa gcaacgggcg tattgttaac ctgaagttat caaaagggca
     5941 gaaaaaacgc gtggcgctgt tgctggcgct ggcagaagaa cgcgatatta tcctgctgga
     6001 tgaatgggcg gcggatcagg atccacactt ccgtcgtgag ttttatcagg tgttgctgcc
     6061 gctgatacag gagatgggta aaactatttt cgctatcagt catgatgatc attactttat
     6121 ccacgccgac cgcctgctgg aaatgcgcaa tgggcaactt agcgagctga cgggcgaaga
     6181 gcgcgatgcc gcttcgcgtg atgccgttgc ccggacggca taatattacg acgctgacct
     6241 ggcggactgc tgccgtcagg tcaatatgct aaaaatccct tcatttctaa taaccctata
     6301 attaatcaac gaaattataa tgtttctaaa attagaatat aatttataaa cattatttaa
     6361 atgttgttac ttaagtgtta ccgttgatgc cgcgcaaatc tcacctgcaa taaagcgact
     6421 aaaagtaagg cattaacaag atgaaaaaag tgactgccat gctcttctcg atggccgtgg
     6481 ggcttaatgc cgtttcgatg gcggcaaaag cgaaagcgtc cgaggagcag gaaactgatg
     6541 tactgttgat tggcggcggc attatgagcg ccacgttggg gacctattta cgcgagctgg
     6601 agcctgaatg gtcgatgacc atggtggagc gcctggaggg tgtcgcgcag gagagttcga
     6661 acggctggaa taacgccgga accgggcatt ctgcactgat ggaactgaac tacaccccgc
     6721 aaaacgccga tggcagcatc agtattgaaa aagcagtcgc cattaacgaa gcatttcaga
     6781 tttcccgcca gttctgggcg caccaggttg agcgcggcgt gctgcgtact ccgcgttcat
     6841 ttatcaatac cgttccgcat atgagctttg tctggggcga ggataacgtt aatttcctgc
     6901 gcgcccgtta cgccgcgttg caacaaagct cgctgtttcg cggtatgcgt tactctgaag
     6961 atcacgcgca gatcaaagag tgggcaccgt tagtgatgga agggcgcgat ccacaacaga
     7021 aagtagcggc aacacgcaca gaaattggca ctgatgtgaa ctatggcgag atcacccgcc
     7081 agttaattgc ttccttacag aagaaatcta acttctcgct gcaactcagc agcgaagtcc
     7141 gcgccctaaa gcgtaatgac gataacacct ggaccgttac cgttgccgat ctgaaaaacg
     7201 gcactgcaca gaacatccgc gccaaatttg tctttatcgg cgcgggcggt gcggcgctga
     7261 agctattaca ggaatcgggg attccggaag cgaaagacta cgccggtttc ccggtgggcg
     7321 gacagttcct tgtttcggaa aacccggacg tggttaatca ccatctggcg aaggtttacg
     7381 gtaaagcatc cgttggcgca ccaccgatgt cggttccgca tatcgatacc cgcgttctgg
     7441 acggtaaacg cgtagtgctg tttgggccat ttgccacctt ctcaaccaaa ttcctcaaaa
     7501 acggttcatt gtgggatctg atgagttcca ccaccacctc taacgtgatg ccgatgatgc
     7561 acgtcgggct ggataatttc gatctggtga aatatctggt gagtcaggtg atgttgagtg
     7621 aagaggatcg ttttgaagcg ttgaaagagt actatccgca ggcaaaaaaa gaggactggc
     7681 gtttgtggca agcggggcag cgcgtgcaga ttatcaagcg tgatgccgag aaaggtggcg
     7741 tactgcgtct gggtactgaa gtggtcagtg accagcaagg gaccattgcc gcgctgctgg
     7801 gggcatcgcc aggggcgtca accgccgcgc cgattatgtt ggatctgctg gaaaaagtat
     7861 ttggcgatcg cgtttccagc ccgcaatggc aggctacgtt gaaagcgatc gttccgtctt
     7921 atggacgcaa gctgaacggt gatgtagcgg caacagaacg cgagttgcag tacaccagcg
     7981 aagtgctggg gctgaaatac gacaagccgc aggcagcaga tagtacgcca aaaccgcagt
     8041 tcaaaccgca acccgttcaa aaagaagtgg cggatattgc gttgtaatga tgtgccacat
     8101 ccggcatggt atgccggatg tggcgtatgc tgataagacg cgccagcgtc gcatcaggca
     8161 ccggctcggg cgttagatgt cggatgcggc gtgaccgcct tattcggcct acggggtgcc
     8221 gcacttcaca gcattttagc gaactaccgc gttgtcaatt ttctcttccg ccttccagac
     8281 gcggtacttc acatctacat tgtctggcgt atacaccacg atcggcagct tgctgttgta
     8341 acgcagcatt ccagcatcgc ccagatacgc ggtgacaaat ttcttctctt tcttgccatc
     8401 cgggcaggcc atcatcgtgg aaaccggcga gctaacttta tcaaagacat aataatcata
     8461 gccccagcct tccagcgttt tgctttccag cttcccgccg agacgatgca aattgcaatc
     8521 gacttccagc gtctgaccga ttaacagttc tactttcagg gtagattcat cttcttgcgg
     8581 ggttaactga atcacctgac gcttcatccc tttttcagct tgtggataag gcgcgatttt
     8641 ttccagtggc tggacgcttt ctgccgccca ggcggaagtg gtagcgaaag cggcaaacaa
     8701 tactgcaggt agaatggtct tcattttttt gctttccttc ttcatttata ggttttcacc
     8761 gatgatgtcg ctgaaggtta acatattcat cagatttatt aatttgcttt atgaataact
     8821 taaagaacat tcagacaatt ttacctgctc ttttagtgtt aaattctggt gagggagcaa
     8881 accagcagaa atatctctgg tgaattaatc gctatataaa tatgtttata acaatttgaa
     8941 atgtgagcaa aagcccgttt ttgccacgct tcccgccact cttttgatcc tgctacaggt
     9001 tttaccccga tcggggtatg catctttgac acatccttta atatcttagc ggctataaaa
     9061 atggcttatt aattatgcgg tttatttggc cgctctcaat tttcagagcg cgttaatgat
     9121 ggaaggtcaa tgtgaagatt gatgcatccc gtcggggcat actcactggt cgctggcgca
     9181 aagccagtaa cggtatccgt ccgccttggt ccggtgatga atctcatttt ctgacccatt
     9241 gcacccgttg cgacgcctgt attaatgctt gtgaaaacaa cattctgcaa cgcggcgcgg
     9301 gcggctatcc gagcgttaat ttcaaaaata acgagtgcag cttctgttac gcctgcgcgc
     9361 aggcttgccc cgaatcgcta ttttctccgc gccacaccag ggcctgggat ttgcagttca
     9421 ccattgggga cgcgtgcctg gcgtatcagt cagttgaatg ccgccgctgt caggatagct
     9481 gtgagccaat ggcaatgatc tttcgcccga cgctctccgg gatctaccag ccgcaactta
     9541 atagccaact ttgcaacgga tgtggagcat gtgcagccag ctgtccggta tcagccataa
     9601 ctgcggagta tctccatgca cactaactgg caagtttgca gcctggtcgt gcaggccaaa
     9661 agcgaacgaa tttcagacat cagcacccaa ctgaacgcct ttcccggctg tgaagttgct
     9721 gtcagcgacg cgccgagcgg tcagttgatt gtggtggtgg aagcagaaga cagcgaaacg
     9781 ctgatccaaa ccattgagtc agtacgcaac gtagagggcg tgctggcggt gtcgctggtt
     9841 tatcaccagc aggaagagca aggtgaggaa acaccatgaa actcagtcgt cgtagcttta
     9901 tgaaagctaa cgccgttgcg gccgctgcgg cggctgccgg tctcagcgtg ccgggcgttg
     9961 cccgcgccgt tgttggtcag caggaagcca ttaaatggga taaagcgccg tgccgtttct
    10021 gcggtactgg ttgcggcgtt ctggtcggaa cgcagcaggg gcgtgtggtg gcctgtcagg
    10081 gcgacccgga cgcaccggtt aaccgtggcc tgaactgcat taagggctat ttcctgccca
    10141 agatcatgta cggtaaagac cgtttgacgc agccgctgct gcgtatgaaa aacggtaaat
    10201 atgacaaaga aggcgaattt accccaatca cctgggatca ggccttcgat gtgatggaag
    10261 agaaattcaa aaccgccctg aaagaaaaag gaccggaatc gatcggtatg ttcggttctg
    10321 gtcagtggac tatctgggaa ggttatgccg cgtccaagct gttcaaagcg ggcttccgtt
    10381 cgaacaacat cgacccgaac gcacgtcact gtatggcgtc ggcagtagtt ggctttatgc
    10441 gtacctttgg tatggatgag ccgatgggct gctatgacga catcgagcag gctgacgcgt
    10501 ttgtgctgtg gggcgctaac atggcggaga tgcacccgat cctctggtca cgcatcacta
    10561 accgtcgtct ctctaaccag aacgtcaccg tggcggtgct ttctacctac cagcatcgta
    10621 gcttcgagct ggcggataac ggcatcatct ttacgccaca gtctgacctg gtgatcctga
    10681 actacatcgc caactatatc attcaaaaca atgcgataaa tcaggacttc ttcagcaagc
    10741 acgttaacct gcgcaaaggg gcgacggaca tcggctacgg tttacgtccg acccatccgc
    10801 tggaaaaagc agcgaagaat ccgggttctg acgcctccga accgatgagc tttgaagatt
    10861 acaaagcctt cgttgctgag tatacgctgg aaaaaaccgc tgaaatgacc ggcgtgccga
    10921 aagaccagtt agaacaactg gcgcagctgt atgccgatcc gaacaagaaa gtcatctcct
    10981 actggacgat gggcttcaac cagcataccc gtggtgtgtg ggccaacaac ctggtctaca
    11041 acctgcacct gctgaccggc aaaatttccc agccgggttg cggtccgttc tccctgaccg
    11101 ggcaaccttc tgcgtgtggt actgcgcgtg aagtgggcac ctttgctcac cgtctgcctg
    11161 cggacatggt ggtgactaac gagaaacacc gtgatatctg tgagaagaag tggaatatcc
    11221 cgagcggcac cattccggcg aaaatcggtc tgcacgcggt ggcacaagac cgtgcgctga
    11281 aagacggcaa gctgaatgtt tactggacca tgtgtaccaa caacatgcag gccgggccga
    11341 acatcaatga agagcgtatg ccgggctggc gcgatccgcg caacttcatc atcgtctccg
    11401 atccgtatcc gacagtcagt gcgctggccg ccgacttgat cctgccgacc gcaatgtggg
    11461 tagagaaaga gggcgcttac ggtaacgccg aacgccgtac tcagttctgg cgtcagcagg
    11521 tacaggcgcc aggcgaagcg aaatcggatc tctggcagtt agtccagttc tcccgccgct
    11581 tcaaaactga agaagtatgg ccggaagagc tgctggcgaa gaaaccggaa ctgcgtggca
    11641 aaacgctgta cgaagttctg tatgccaccc ccgaagtgag caaattcccg gtatccgaac
    11701 tggcggaaga tcagctgaac gatgaatccc gcgagctggg cttctatctg caaaaagggc
    11761 tgttcgaaga gtacgcatgg tttggtcgcg gtcacggtca cgatctcgcg ccgttcgatg
    11821 actaccacaa agcgcgcggt ctgcgctggc cggtggtgaa cggtaaagaa acacagtggc
    11881 gttacagcga aggtaacgac ccgtacgtga aagcgggcga aggctataag ttctacggta
    11941 aaccggatgg caaagcggtg atcttcgcac tgccgttcga accggcggcg gaagcaccgg
    12001 atgaagagta cgacctgtgg ctctctaccg gacgcgttct ggagcactgg cacaccggca
    12061 gtatgactcg ccgtgtgccg gaactgcacc gcgccttccc ggaagcggtc ttgtttattc
    12121 acccgctgga tgcgaaagcg cgcgatctgc gccgtggcga caaagtgaaa gtggtttctc
    12181 gccgtggcga agtgatctcg attgttgaaa cgcgcggtcg taaccgtccg ccacagggcc
    12241 tggtgtacat gccgttcttc gacgccgcac agctggttaa caaactgacg ctggatgcga
    12301 ccgatccgct ctcgaaagag acggacttca agaagtgcgc ggtcaaactg gagaaggtgt
    12361 aagccattat gtcccggtca gcgaaacctc aaaatggtcg ccgccgcttt ctgcgcgatg
    12421 tcgttcgcac agcaggcggg ctggctgccg tgggtgtggc gctggggtta caacagcaaa
    12481 ccgcacgcgc atctggcgtg cggttgcgcc cgcccggagc cataaacgag aacgcctttg
    12541 ccagtgcctg tgtgcgttgc ggtcagtgtg ttcaggcttg cccttacgac accttaaaac
    12601 tggcgacgct ggcttctggc ctgtcggcgg gcacgccata ttttgtcgca cgggatattc
    12661 cttgcgaaat gtgtgaggac attccgtgcg ccaaagtgtg cccaagcggt gcgctggatc
    12721 gtgagattga atcgatcgac gacgcgcgga tggggctggc ggtactggtg gaccaggaaa
    12781 actgtctcaa ctttcagggg ctgcgctgcg atgtttgtta tcgcgaatgc ccgaaaattg
    12841 atgaggccat caccctggag ctggagcgca acacgcgtac cggtaagcac gcccgctttc
    12901 tgccgacggt tcacagcgac gcctgtaccg gttgcggtaa gtgcgaaaaa gtatgcgtgc
    12961 tggaacaacc ggcaatcaag gtgttaccgc tgtcactggc gaaaggggag ttaggtcacc
    13021 attaccgctt cggttggctg gaggggaaca atggcaaatc gtaaacgtga cgccgggcgc
    13081 gaggcgctgg agaaaaaagg ctggtggcgc agtcaccgtt ggctggtgtt acgtcgcctt
    13141 tgtcagttct tcgtgctggg gatgtttttg agcggtccat ggtttggtgt gtggatcttg
    13201 cacggcaact acagcagtag cctgttattc gacaccgttc cgctgaccga tccgctgatg
    13261 acgctgcaaa gtcttgccag tggtcatctg cctgccacgg tggcgttgac cggtgcggtg
    13321 attatcaccg tgctctacgc cctggcggga aagcgattat tttgcagctg ggtctgcccg
    13381 ctgaacccga ttaccgactt agcgaactgg ttacgcaggc gctttgacct caatcagtct
    13441 gcgacgatcc ctcgccacat tcgctacgtg ctgttggtgg tcattctggt gggatcagcc
    13501 ttaactggca cgctcatttg ggaatggatc aaccccgttt ctctgatggg gcgtagcctg
    13561 gtaatgggct tcggcagcgg cgcgctgctt attctcgcgc tgtttttatt tgatctactg
    13621 gtcgttgaac acggctggtg cgggcacatt tgtccggttg gcgcgctgta tggcgtgctg
    13681 ggtagcaaag gtgtgattac cgttgcggcc accgatcggc agaaatgtaa ccgctgtatg
    13741 gattgttttc atgtttgccc ggaaccgcat gtgctacgtg ccccggtgct ggatgaacaa
    13801 agcccggtgc aggtcaccag ccgcgattgc atgacgtgcg gtcgctgcgt ggatgtctgt
    13861 tctgaggatg tatttacaat aactacacga tggagttcgg gagcgaaatc atgaaaagcc
    13921 atgacctgaa gaaagcgctg tgtcaatgga cggcgatgct ggccctggtg gtaagcggcg
    13981 cggtttgggc ggctaatggc gtcgatttta gccagtcgcc ggaagtatcc gggacacagg
    14041 aaggggccat tcggatgcca aaagagcagg aacggatgcc gctgaactat gtgaatcagc
    14101 cgccaatgat cccgcatagc gttgaaggtt atcaggtaac gaccaatacc aaccgctgct
    14161 tgcagtgcca cggtgtcgaa agttatcgca ccactggcgc gccgcgtatc agtcctactc
    14221 actttatgga cagcgacggc aaagtgggcg cggaagtggc tccgcgtcgt tatttctgtc
    14281 tgcaatgtca cgtaccgcag gccgataccg cgccaatcgt ggggaatacc tttaccccat
    14341 caaaaggtta cgggaaataa gaggttatta tgggaaattc tgaccgtaag cctggtctga
    14401 ttaagcgcct gtggaaatgg tggcgtaccc ccagccgtct ggcgctgggg acgctgctgt
    14461 tgatcggttt tgttggcggc atcgtcttct ggggtggctt taacaccggg atggaaaaag
    14521 ccaataccga agagttctgc attagctgcc acgaaatgcg caacacggtg tatcaggaat
    14581 acatggattc cgtgcactac aacaaccgta gcggcgtccg tgcgacctgt ccggattgtc
    14641 acgttccgca cgagtttgtg ccgaagatga tacgcaagct caaagcaagt aaagagctgt
    14701 atggtaaaat ttttggcgtt attgacacgc cgcagaaatt tgaagctcat cgtctgacga
    14761 tggcacagaa tgagtggcgg cgcatgaagg acaataactc gcaggagtgc cgtaactgtc
    14821 acaacttcga gtatatggat acaaccgccc agaaatcggt tgccgcgaag atgcatgacc
    14881 aggcggtgaa agatgggcaa acctgtattg attgccataa agggatagcg cacaagctgc
    14941 ccgatatgcg tgaagtcgag ccaggttttt aacagggtta ttgcgtgggt atgcttgaag
    15001 ccagagagtt actttgtgag cgggatgaac gaaccttatt tagtggcttg tcatttacgc
    15061 tgaacgcagg agagtgggta caaatcaccg gtagcaacgg tgcggggaag acaacgcttc
    15121 tccgtttgct gacggggttg tctcgccctg acgcaggcga ggttctctgg caagggcagc
    15181 ccttgcatca ggtacgcgac agctaccatc aaaacctgtt atggataggc catcagccgg
    15241 ggatcaaaac ccggctgacg gcgttagaaa atctgcactt ttatcatcgc gatggcgata
    15301 ccgcacaatg tctggaagcc ctggcgcagg ccgggcttgc cggattcgaa gatattcctg
    15361 taaatcagct ctcggccggg caacaacgcc gcgtcgcttt agcgcgtctg tggctgaccc
    15421 gtgccacgtt atggatcctc gacgagcctt ttaccgcgat tgacgttaac ggcgtcgatc
    15481 gtctgaccca gcgtatggcg cagcatacgg agcagggggg gattgtgatt ctgactaccc
    15541 accagccgct caacgttgct gaaagtaaaa ttcgccgcat ttcactgacg caaacgaggg
    15601 ccgcatgatg ttctggcgca ttttccgtct tgagctgcgt gtagcgtttc gccatagcgc
    15661 cgaaatcgcc aacccgctgt ggttcttcct gattgtaatt accctttttc cgctcagtat
    15721 cggtccggag ccgcaactgc tggcgcgtat tgcaccgggc attatctggg ttgctgcgct
    15781 gctttcatcc ttgctggcgc tggaacgact gttccgtgac gatttgcagg acggcagtct
    15841 tgaacaattg atgttgttgc cgttaccctt gcccgccgtt gtgctggcga aggtgatggc
    15901 gcactggatg gtaaccggtc tgccgttact catcctttcg ccactggtag caatgctact
    15961 gggaatggat gtttatggct ggcaagtgat ggcgctgacg ctgctgctgg gaacgcctac
    16021 gcttggcttt ctcggtgcac cgggcgtggc gctgacagtg ggacttaagc gcggtggtgt
    16081 gctgctcagc atactggtgt taccgctgac tatcccatta ctcatcttcg ccaccgccgc
    16141 gatggacgcg gcttccatgc atttgcccgt tgacgggtat ctggcaattt taggcgcgtt
    16201 gctggcatgc accgcgacat taagtccttt tgcgacggcg gcagcgttac gaatcagcat
    16261 tcaataacgc tggattactg atttttgttc gagtctggta tcgaaactat gtggaaaaca
    16321 ctgcatcaac tggcgatccc accacggctg tatcaaatct gtggctggtt tataccgtgg
    16381 ctggcaattg ccagtgtggt cgtgcttacc gtcggctgga tctggggatt cggctttgct
    16441 ccggctgatt atcagcaggg aaatagctac cgcattatct acctgcatgt gcctgcggcg
    16501 atctggtcga tgggcattta tgcatcaatg gcggtggcgg cttttattgg ccttgtctgg
    16561 cagatgaaaa tggccaacct ggcggtggcg gcgatggccc ccattggtgc cgtgtttacc
    16621 tttattgccc tggttaccgg ctctgcatgg ggaaaaccga tgtggggcac ctggtgggta
    16681 tgggatgcac gtctgacttc tgaactggtg ctgctgtttt tgtatgtggg tgtgattgcc
    16741 ctgtggcacg ccttcgacga ccgccgtctg gcgggccgtg cggcaggtat cctggtgctg
    16801 attggcgtgg tgaatctgcc gattattcat tactccgtgg agtggtggaa caccctgcat
    16861 cagggatcaa cgcggatgca gcaaagtatc gatccggcga tgcgttcgcc gctgcgctgg
    16921 tcgatttttg gcttcctgct gctgtctgcc acgctgacgc tgatgcggat gcgtaatttg
    16981 attttgctga tggaaaaacg ccgtccgtgg gtgagtgaac tgatactgaa aagaggccgt
    17041 aaatgacccc tgcatttgct tcctggaatg aatttttcgc aatgggcggt tacgcctttt
    17101 ttgtctggct ggcggtggtg atgaccgtta ttccgctggt ggttttggtc gtgcactcgg
    17161 tgatgcagca tcgcgcaatt ctgcgtggtg tggcgcaaca gcgggcgcgt gaggcgcgtt
    17221 tacgtgctgc gcaacagcag gaggctgcat gaatattcgc cgtaaaaacc gcttgtggat
    17281 tgcctgtgcc gtgttggcag ggctggcgct gactatcggt ctggtgctat atgcgctgcg
    17341 ctcgaatatc gatctctttt atacgccggg ggaaattctc tacggcaagc gtgaaactca
    17401 gcaaatgccg gaagtcgggc agcgtctgcg cgtcggcggg atggtgatgc cgggtagcgt
    17461 gcagcgcgat cccaattcgc taaaagtaac tttcaccatt tacgacgctg aaggttcagt
    17521 agatgtctct tacgaaggca ttttgccgga tctgttccgt gaagggcagg gcgttgtggt
    17581 gcagggcgaa ctggaaaaag gcaatcatat cctcgcaaaa gaagtgctgg cgaaacacga
    17641 cgaaaactac acgccgccag aagttgagaa agcgatggaa gccaatcacc gtcgcccggc
    17701 gagtgtttat aaggacccag catcatgatg ccggaaattg gtaacgggct gctgtgtctg
    17761 gcgctaggaa ttgcgctgct gctgtccgtg tatccgctat ggggcgtagc gcgcggagat
    17821 gcgcgcatga tggcgtcttc ccgcttgttt gcctggctgc tgtttatgtc tgtggctggc
    17881 gcatttctgg tactggtcaa tgccttcgtg gtcaacgact tcaccgtcac ctatgttgcc
    17941 agcaactcca atacccagct tccggtgtgg tatcgcgtgg cggctacctg gggcgcgcat
    18001 gaaggctcgc tcctgctgtg ggtgctgctg atgagcggct ggacctttgc ggtagcgatt
    18061 tttagtcagc gtattccgct ggatattgtg gcccgcgtac tggcgataat ggggatggtc
    18121 agcgtcggct ttttgctgtt cattctcttt acctctaacc cgttctcacg cacgttgccg
    18181 aacttcccga ttgaagggcg cgatcttaac ccgctgttac aggatccggg gctgatcttc
    18241 catccgcctc tgctctatat ggggtacgtg gggttctcgg tggcgtttgc ttttgccatt
    18301 gcttctttgt tgagcgggcg tctggacagc acttatgcgc gttttactcg tccgtggacg
    18361 ctggcggcgt ggatcttctt gacgctcggc attgtgctcg gttccgcatg ggcctattac
    18421 gaactcggct ggggcggctg gtggttctgg gatccggtag aaaacgcctc gtttatgccg
    18481 tggctggtgg ggactgcgct gatgcactca ctggcggtca ctgaacaacg cgccagcttc
    18541 aaagcgtgga cattactgct ggcaatcagt gccttctcgt tgtgtctgct ggggactttc
    18601 ctggtgcgtt ccggcgtgct ggtatcggta cacgcgtttg cctctgatcc ggcacgcggt
    18661 atgtttatcc tcgcctttat ggtactggtg attggtggtt cgctgctgct gtttgccgcg
    18721 cgtggacaca aagttcgctc acgcgtaaac aatgcgctgt ggtcgcggga atctctgctg
    18781 ttagcgaaca acgttttgct ggtcgccgcg atgctggtgg tattactggg gacgctgctg
    18841 ccgctggtgc acaagcaact gggactgggc agtatttcga ttggcgaacc gttcttcaac
    18901 accatgttta cctggctgat ggtgccgttt gcgctgctgc ttggtgtcgg tcctctggtg
    18961 cgctgggggc gcgatcgccc acgtaaaatc cgcaatttat tgattatcgc cttcatctca
    19021 acgctggtgc tgtcgctgct gctgccgtgg ctgttcgaaa gcaaagttgt ggcgatgacg
    19081 gtgctcggcc tggcaatggc ctgctggatt gctgtgctgg caattgcgga agctgcgctg
    19141 cgtatttcac gtggcacgaa aaccaccttc agttattggg gaatggtggc ggctcacctg
    19201 gggctggcag tgacaattgt tggtattgcc tttagccaga actatagcgt tgagcgtgat
    19261 gtgcgcatga agtccggcga tagcgtcgat attcatgaat atcgcttcac cttccgtgat
    19321 gtcaaagagg tgactggccc gaactggcgt ggcggtgtgg cgactatcgg cgtaacgcgc
    19381 gatggcaagc cggaaacggt gctgtatgcg gaaaaacgtt attacaacac tgccgggtcg
    19441 atgatgaccg aagcggcaat tgacggcggc atcacgcgtg acctgtacgc cgcgctcggt
    19501 gaagagctgg aaaacggcgc gtgggccgtg cgtctttact acaaaccatt tgttcgctgg
    19561 atttgggcgg gcgggctgat gatggcgttg ggcggactgc tgtgtctgtt tgatcctcgc
    19621 tatcgtaagc gcgtgagtcc gcaaaaaact gcgccggagg ccgtatgaag cgcaaagtat
    19681 tgttaattcc gttgattatc ttcctggcga ttgccgcggc gctgctgtgg cagctggcgc
    19741 gtaatgccga aggggatgat ccgaccaatc tggaatcggc gctcattggc aagcctgtgc
    19801 cgaagtttcg tctcgaatca ctggacaatc cggggcagtt ttatcaggcg gatgtgctga
    19861 ctcagggcaa accagtgctg cttaacgtct gggcgacctg gtgtccgacc tgccgtgcgg
    19921 aacatcaata tctgaatcag ctttctgcgc agggcatccg agtggtcggc atgaactata
    19981 aagacgatcg ccagaaggca atcagctggc tgaaagagct gggcaatcct tacgcgctaa
    20041 gcctgtttga tggcgacggc atgttagggc tggatctcgg tgtctatggc gcgccagaaa
    20101 ccttccttat tgacggcaac ggcatcattc gctatcgcca tgcgggcgat ctcaatcctc
    20161 gcgtctggga agaagagatc aagccgctgt gggagaaata cagtaaggag gccgcacaat
    20221 gaggttttta ttgggcgtgc tgatgttgat gatctccggc tcagcgctgg cgaccatcga
    20281 cgtgttgcag tttaaagatg aagcacagga acaacagttc cgtcagctca ctgaagaact
    20341 gcgctgcccg aaatgccaga ataacagcat tgccgattcc aactcgatga ttgccaccga
    20401 cctgcgccag aaagtgtatg aactgatgca ggaaggtaaa agtaagaaag agattgtcga
    20461 ttatatggtg gcgcgttacg gcaacttcgt cacttacgat ccgccgttaa cgccgctgac
    20521 cgtgctgctg tgggtgctgc cagtagtggc tattggcatt ggcggttggg tcatttacgc
    20581 ccgctcgcgg cgtcgggtac gcgtggtgcc ggaagcgttt cctgaacaaa gcgtgccgga
    20641 aggtaagcgt gccggatatg tcgcttattt gccgggcatt gtggtggcgt taattgtggc
    20701 tggcgtcagc tactaccaga ctggcaatta tcagcaggtg aaaatctggc agcaggccac
    20761 ggcacaggct ccggcgttgc tggacagggc gctggatccg aaagccgatc cgctcaacga
    20821 agaagagatg tcgcgtcttg cgctggggat gcgtactcaa ctgcaaaaaa atccgggaga
    20881 tatagaaggc tggattatgt tgggccgcgt tggcatggcg ctgggtaacg ctagtatcgc
    20941 caccgatgca tacgctactg cgtatcgcct cgatccgaaa aacagcgatg ccgcactggg
    21001 ttatgctgaa gcgttgacac gttcatctga tcccaacgac aaccgcctcg gcggcgaact
    21061 gctgcgccag ttggtgagaa gcgaccacag caatatccgt gtgttaagca tgtatgcgtt
    21121 taatgccttt gagcagcagc gatttggcga agccgttgcc gcgtgggaga tgatgttgaa
    21181 actcttacct gccaacgata ctcgccgtgc ggtgattgaa cgtagtatcg cgcaggcgat
    21241 gcaacatttg tcgccgcagg agagtaaata aaaagaggcc cgatggtgat gccatcgggc
    21301 tatttttatt gtgccccgcg ttgttgcagg aacagaatgg tggctgccac gcgtgagcgg
    21361 acattgagtt tgcgcagcag attgcgaata tgtactttta ctgtctgctc tgaaatattc
    21421 aacaccgagg caatctgttt atttgacagc ccctgtgcca gctcgtgcag aacatccagc
    21481 tcgcgctccg tcagcacgct gaagggatct tcttccgcgc caaacatttc acgttcacgt
    21541 aagtactgat tgacgcgttc gctaaagact ttgctgcctt tcgctccagc acgaattgct
    21601 tccagcaata cttccgggtc gctgtctttc aacagataac cgtctgcgcc tgcgtctatc
    21661 agtgcaaaga catcgctgga ggcatctgat acggtcagga taataatttg cgcggtaacg
    21721 ccatccctgc gcaaggcatt gagggtatcc aggccactca tacctttcat attgagatcc
    21781 agcaagatca cgtcgatatc cagtctattc gccagatcga tcgcgctcgc gccgtcgccc
    21841 gcttcggcga ccacttcaaa gccaggatca agctccagta actgacgaac accgcgtcgc
    21901 ataagtggat gatcatccac aatcatcacc tgaaaaggcg ttgcttcagg catagtagtc
    21961 tcctgaggtt ttattagaat cattatgttt gagcgtagcg ttggtaacgc taccaggcgc
    22021 gtgaatgtta ccccatttta tgtatggaca tgtagtgaaa actacaacct tgtcgttcgc
    22081 tttggcatca ttttcattat aaagacggaa ttttgcatca gcttctcatc aagaaaaaca
    22141 actaaaaaat caatcacaat acttgtgcat ttctttaaac agtttttaaa aactgactcc
    22201 gggtatggag ctatgggtat tttccgtacc caatgctttt aacagcaatt aatttcatag
    22261 gatgaaagct caatgcatca atctggttct gtttctcttt gtcgttccgc aatatctgtt
    22321 ctggtggcta cagcgttata ttcacccata gcattggcat caacagttga gtatggtgag
    22381 acagttgatg gtgttgtcct ggaaaaagat atccagctgg tttatgggac cgccaataat
    22441 acgaaaatca atcctggcgg agaacagcat ataaaagaat ttggtgtaag taataatact
    22501 gaaattaacg gagggtatca gtacattgaa atgaatggcg ccgcagaata ctcagtatta
    22561 aatgacggtt atcaaattgt tcaaatgggt ggcgcggcaa accagactac gctcaataat
    22621 ggtgtgctac aggtttatgg cgcagcgaat gataccacga ttaaaggcgg gcgcttaatc
    22681 gttgaaaaag atgggggggc cgtctttgtc gctatcgaaa agggaggact actggaggtt
    22741 aaagaggggg gattagcgat tgcggtagat cagaaagcag gcggtgctat taaaacaacc
    22801 acgcgggcca tggaggtatt cggaacaaac cgtctcggtc agttcgatat caagaatggt
    22861 attgctaata atatgttgtt ggaaaacggc ggaagtttgc gagttgaaga aaatgacttc
    22921 gcttataata ccactgtaga tagtggcggc ttactggagg ttatggatgg cgggactgta
    22981 actggcgttg ataaaaaagc aggcggaaaa ttaattgtct caacgaatgc gctggaagtg
    23041 agtggtccaa acagtaaagg ccaatttagt ataaaagatg gtgtgtcaaa aaattatgaa
    23101 ctggatgatg gttccgggct cattgttatg gaggacacgc aggccattga tactatcctt
    23161 gatgagcatg ccactatgca atcgctggga aaggatactg gtacgaaagt gcaggcaaat
    23221 gcggtatatg atctcggtcg atcagatcag aatggaagta tcacgtattc ctcaaaagcc
    23281 atctctgaaa atatggttat caacaatggc cgcgctaacg tctgggctgg cacaatggtt
    23341 aacgtttcag tcagagggaa tgatggcatt cttgaggtca tgaagccgca aataaattat
    23401 gcacccgcaa tgttggtggg taaggtagtg gtttctgagg gcgcttcttt tagaacgcat
    23461 ggtgccgtgg ataccagcaa agcggacgtt tcgctcgaaa atagtgtatg gaccatcatt
    23521 gccgatatca ctacgacgaa ccaaaacacc ctcctcaact tagccaacct tgcgatgtct
    23581 gacgcaaatg tgattatgat ggatgagcca gtgactcgtt catcagtgac ggcaagtgcg
    23641 gaaaatttca ttacgttgac caccaatacc ctgtcgggaa acggcaattt ttatatgcgt
    23701 accgatatgg ctaatcatca gagcgatcag ctcaacgtca ccggtcaggc aacaggtgat
    23761 ttcaaaatat tcgtgacgga caccggtgcc agcccggcag caggagatag ccttacactg
    23821 gtaacaacgg gcggcggtga tgctgcattt acgttgggca atgccggagg cgttgttgat
    23881 atcggtacgt atgaatatac cttgctggat aatgacaacc atagctggag tttggcagag
    23941 aatcgcgcgc aaattacccc ttcaaccact gatgtgctga atatggcggc agcacaaccg
    24001 ctggtatttg atgcagaact ggacaccgtg cgtgagcgtc ttggtagcgt aaaaggcgtt
    24061 aattacgata cggcgatgtg gagttcggca attaacaccc gcaacaacgt gaccactgat
    24121 gcgggcgctg gctttgagca aacattgacc gggctgacgc tcggtatcga tagccgtttc
    24181 tcccgtgaag aaaccagcac aattcgcggc ttgttctttg gttactctca ttctgatatt
    24241 ggttttgatc gcggcggcaa aggcaatgtc gatagctata ccctgggggc ttatgccggt
    24301 tgggagcatc agaacggtgc ctatgttgat ggagtggtga aagttgaccg ttttgccaac
    24361 accatccatt gcaagatgag taatggggca acagcgtttg gcgattacaa tagtaacggc
    24421 gcgggtgctc atgtcgagag cgggttccgt tgggttgacg gattgtggag tgttagaccc
    24481 tatctggcct ttaccggctt taccacagat ggtcaggact acacgttatc aaacggcatg
    24541 cgcgcggatg tgggaaatac ccggatatta cgcgctgaag cgggaacggc ggtaagctat
    24601 cacatggacc tgcaaaacgg tacgacgctg gaaccctgac tgaaagccgc cgtgcgtcag
    24661 gaatacgccg attctaacca ggtgaaagtt aatgacgatg gcaaatttaa taatgatgtg
    24721 gctggaaccc gtggcgttta tcaggctggg ataaggtcat cgtttacccc gacgttaagc
    24781 ggtcatttgt cagtcagcta tggcaatggc gcaggggtag aatcgccgtg gaatactcag
    24841 gcgggtgtgg tctggacgtt ctgataacaa aaaataaaca ggctgtgatg tgtcacggtc
    24901 tgtttatcga attaattgca gatataaaaa aaaccaaccg taaaggttga ttttttcttg
    24961 ggatttttgg
//
LOCUS       DAZFRN010000068        24597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFRN010000068 DAZFRN010000000
VERSION     DAZFRN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.068"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(102..185)
                     /gene="yohP"
                     /locus_tag="ACHKUB_002616"
     CDS             complement(102..185)
                     /gene="yohP"
                     /locus_tag="ACHKUB_002616"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO6655618.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            409..1844
                     /gene="mdtQ"
                     /locus_tag="ACHKUB_002617"
                     /pseudo
     CDS             409..1844
                     /gene="mdtQ"
                     /locus_tag="ACHKUB_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            1897..2658
                     /gene="yohF"
                     /locus_tag="ACHKUB_002618"
     CDS             1897..2658
                     /gene="yohF"
                     /locus_tag="ACHKUB_002618"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6655619.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSYVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2788..3366)
                     /gene="yohD"
                     /locus_tag="ACHKUB_002619"
     CDS             complement(2788..3366)
                     /gene="yohD"
                     /locus_tag="ACHKUB_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6655620.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            3536..4123
                     /gene="yohC"
                     /locus_tag="ACHKUB_002620"
     CDS             3536..4123
                     /gene="yohC"
                     /locus_tag="ACHKUB_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO6655621.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4297..5229
                     /gene="pbpG"
                     /locus_tag="ACHKUB_002621"
     CDS             4297..5229
                     /gene="pbpG"
                     /locus_tag="ACHKUB_002621"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO6655622.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5267..6982)
                     /gene="dld"
                     /locus_tag="ACHKUB_002622"
     CDS             complement(5267..6982)
                     /gene="dld"
                     /locus_tag="ACHKUB_002622"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6655623.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            7178..9475
                     /gene="bglX"
                     /locus_tag="ACHKUB_002623"
     CDS             7178..9475
                     /gene="bglX"
                     /locus_tag="ACHKUB_002623"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO6655624.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9686..10603
                     /gene="osmF"
                     /locus_tag="ACHKUB_002624"
     CDS             9686..10603
                     /gene="osmF"
                     /locus_tag="ACHKUB_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO6655625.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10610..11767
                     /gene="yehY"
                     /locus_tag="ACHKUB_002625"
     CDS             10610..11767
                     /gene="yehY"
                     /locus_tag="ACHKUB_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO6655626.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAEALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11760..12686
                     /gene="yehX"
                     /locus_tag="ACHKUB_002626"
     CDS             11760..12686
                     /gene="yehX"
                     /locus_tag="ACHKUB_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO6655627.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            12691..13422
                     /gene="yehW"
                     /locus_tag="ACHKUB_002627"
     CDS             12691..13422
                     /gene="yehW"
                     /locus_tag="ACHKUB_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO6655628.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13403..13510)
                     /gene="yohO"
                     /locus_tag="ACHKUB_002628"
     CDS             complement(13403..13510)
                     /gene="yohO"
                     /locus_tag="ACHKUB_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO6655629.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(13570..14301)
                     /gene="mlrA"
                     /locus_tag="ACHKUB_002629"
     CDS             complement(13570..14301)
                     /gene="mlrA"
                     /locus_tag="ACHKUB_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO6655630.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14523..16208
                     /gene="btsS"
                     /locus_tag="ACHKUB_002630"
     CDS             14523..16208
                     /gene="btsS"
                     /locus_tag="ACHKUB_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO6655631.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            16205..16924
                     /gene="btsR"
                     /locus_tag="ACHKUB_002631"
                     /gene_synonym="yehT"
     CDS             16205..16924
                     /gene="btsR"
                     /locus_tag="ACHKUB_002631"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO6655632.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16971..17441
                     /gene="yehS"
                     /locus_tag="ACHKUB_002632"
     CDS             16971..17441
                     /gene="yehS"
                     /locus_tag="ACHKUB_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO6655633.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17482..17943)
                     /gene="yehR"
                     /locus_tag="ACHKUB_002633"
     CDS             complement(17482..17943)
                     /gene="yehR"
                     /locus_tag="ACHKUB_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO6655634.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(18068..20068)
                     /gene="yehQ"
                     /locus_tag="ACHKUB_002634"
     CDS             complement(18068..20068)
                     /gene="yehQ"
                     /locus_tag="ACHKUB_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO6655635.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRTLQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAVECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(20065..21201)
                     /gene="yehP"
                     /locus_tag="ACHKUB_002635"
     CDS             complement(20065..21201)
                     /gene="yehP"
                     /locus_tag="ACHKUB_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6655636.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(21194..23473)
                     /gene="yehM"
                     /locus_tag="ACHKUB_002636"
     CDS             complement(21194..23473)
                     /gene="yehM"
                     /locus_tag="ACHKUB_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO6655637.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(23484..24572)
                     /gene="yehL"
                     /locus_tag="ACHKUB_002637"
     CDS             complement(23484..24572)
                     /gene="yehL"
                     /locus_tag="ACHKUB_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO6655638.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5730 a         6301 c         6714 g         5852 t
ORIGIN      
        1 tgcgccagtg attatatgaa gcaatgcaca tgacaggagc gcaacaggat gagagactga
       61 aatattctcg ttattttcgg acctgatgac attaattaat tttaaaatat catcttaaat
      121 acgccagtca ccaccagtag cccaatcagg aaaataatca atacagccca gagtataatt
      181 ttcattggcg ttcctttatt tgtgagtgct gaataagtat aggaaaaaac gaagcccttt
      241 gccgttatcc ataaacgatt aagataaatc ggaagatatc attcacttag tgtatatttt
      301 tctggatatg tcgttttatt gctactgatg tattgccacg cgaaactaaa aaatatatcg
      361 caacacacgg taatctccgc gcaccttttc ttacctgatt attcccccat gaatcgtgat
      421 tccttttatc ccgccatcgc ctgttttccg ctgttactga tgctggccgg gtgtgcacct
      481 atgcatgaaa cccgccaggc gttaagccag caaacgcctg ctgcacaagt tgacaccgca
      541 ttacccacgg cgctgaaaaa tggttggcca gacagccaat ggtggctgga gtatcacgat
      601 aatcaactca cttccttaat taacaatgca ctacagaacg caccggatat gcaggtggct
      661 gaacagcgca ttcagctagc tgaagcccag gcgaaggcaa ttgctactca ggatggtccg
      721 cagatcgact tttctgcgga tatggagcgg caaaaaatgt cggcagaagg cttaatgggg
      781 ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc cgtggtacac caacggaact
      841 tttggcttaa cggcgggctg gcatctcgat atctggggaa agaatcgggc ggaggttact
      901 gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg agcaaacccg ccaattgctg
      961 gctggcagcg tagcccgcct gtactgggag tggcaaaccc aggcggcgtt aaacacggtc
     1021 ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga ccgatcgcca gctatatcag
     1081 aacgggatta cttcttcagt tgaaggtgtg gaaaccgata ttaatgccag caaaacccgg
     1141 cagcagctca acgatgtcgc ggggaaaatg aaaattattg aggcacggtt aagcgcactt
     1201 acaaataacc agacaaagtc attgaagctt aaaccggtcg cgttgccgaa agtggcaagc
     1261 cagcttcctg atgaactggg gtactcctta ctggcccggc gggcagattt gcaggcggcg
     1321 cactggtacg ttgagtcatc gctaagcacc attgatgcgg caaaagcggc attttatcct
     1381 gacatcaacc tgatggcctt cctgcaacag gatgcgttgc acttaagcga tctgttccgt
     1441 cattccgcgc agcaaatggg cgttacggca ggcctgacgc tacccatttt cgatagtggt
     1501 cgtcttaacg ccaatctcga tatcgcaaag ccgaaagcaa cttgtctatc gccagctaca
     1561 acaaagcggt ggttgaagcg gtgaatgacg tggcgcgggc agccagtcag gttcagacac
     1621 ttgcggagaa aaaccagcat caggcgcaaa ttgagcgcga tgccttgcgt gtggtaggtc
     1681 ttgcgcaggc gcgctttaac gcgggcatca ttgctggttc ccgcgtcagc gaagccagaa
     1741 tccccgcgct gcgtgagcgg gccaatggcc tgttattgca agggcagtgg ctggatgctt
     1801 ccattcaact cactggtgcg ttgggcgggg gttacaaacg ctgatgatat gcgccttcta
     1861 tacttaacgt ttattcagcg ttaagtggag aactcgatgg cacaggttgc gattattacc
     1921 gcctccgatt cggggatcgg caaagagtgc gcgttattac tggcgcagca ggggtttgat
     1981 attggcatta cctggcactc agatgaagaa ggggcaaaag ataccgcgcg tgaggtagtt
     2041 agccacggcg tacgtgcgga gatcgtgcag ctggatctcg gtaaactgcc agaaggtgcg
     2101 caggcgctgg agaaactcat tcaacggctg gggcgtattg atgtgctggt gaataatgcg
     2161 ggcgcaatga ccaaagcgcc gtttcttgat atggctttcg atgagtggcg caagattttt
     2221 accgttgatg tcgatggtgc atttttatgc tcacaaattg cggcccgcca gatggtgaaa
     2281 caagggcagg gcggtcgcat catcaacatt acgtcggtac atgaacatac gccgctgccg
     2341 gatgccagcg cctacacagc cgctaaacat gcgctcggcg ggttaaccaa agcaatggcg
     2401 ctggagttgg tcaggcataa gattttggtg aacgcagtcg cacctggggc gatcgctacg
     2461 ccaatgaacg gcatggatga tagctacgtg aagcccgacg cggagccttc gattcctctg
     2521 cggcgttttg gcgcaacgca tgagattgcc agcctggtag cgtggctgtg ctcagagggc
     2581 gcaaattaca ccaccgggca gtcgttgata gtggatggcg gctttatgtt ggcgaatccg
     2641 cagttcaacc cagaatagcg cagtgaggtg cgtggttatt tcaatgggta aaattttggt
     2701 aggcatgata agacgcacaa gcgtcgcatc aggcatggtg caccggatgt cggatgcggc
     2761 gcgaacgcct tacccagggc aatggtttta cgcctgatga tccggctttt tcttcccgcg
     2821 ccgtttcagc caccagcgca cgcccaccac cagaaccacg accagaatca accagaccca
     2881 gtgcttcaaa tgctggtcga gattgtgcaa ccacggcgca atcacctgac caccagcgta
     2941 accaatagtg gtaaaaatca acgcccaggc aaatgcgccg agaatattca gcggcagaaa
     3001 gattttcggc ggcagctggc tggcaccaat cagcgtcggg ccaatcaccc gaaagccata
     3061 cataaagcgc gtaccaatga caaacagata cggatggcgt tggataagtt tctgcgcccg
     3121 ctcaatttta tcctgatgtt tcgagaaacg gcgtaacagc ttgccgccaa accgccgccc
     3181 gcacagatag agcacctggt cgccaatcat gccgccaagc gccacagaaa gtaccaccag
     3241 cgggaacttt aatagcccct gatgcgccgc aacgcctccc agcaaagtca cggtttcacc
     3301 ttccgccagg ctaccgatca ccagcgcggc ataaccatat tgtgagataa gtgtattgag
     3361 atccataggc cagtctgtct ccctttggtt gccagtaagc atacaccctg tgaatcattc
     3421 acgctgaaaa aggacgttca tcagacagtg aaaattacat gggcgaacaa aaaataatca
     3481 caagctgact tatacttgaa ctgtcaggtt catgaactca caacaagggg gcggtatgag
     3541 ccatgtctgg ggactgtttt cccatcccga tcgtgaaatg caggtgatta atcgcgaaaa
     3601 cgaaacgatt tctcaccatt acacccacca cgttttgctg atggcggcga ttccggtgat
     3661 ttgcgccttc attggcacta cacagattgg ctggaatttt ggcgatggca ctatcctgaa
     3721 gttatcctgg tttaccggac tggcgctggc tgtcttgttt tacggcgtta tgctggctgg
     3781 ggtcgcggtg atggggcggg tcatctggtg gatggcgcgc aattacccgc agcgtccgtc
     3841 acttgcgcac tgcatggtct tcgcgggcta cgttgcaact ccgctgtttt taagcggtct
     3901 ggtggcgctt tatccgctgg tctggttgtg tgcgctggtc ggcacggtgg cgctgtttta
     3961 caccggctac ctgctttacc ttggtattcc gagcttcctg aatatcaaca aagaggaagg
     4021 ccttagcttc tccagttcga cgctggcgat tggcgtgctg gtactggaag tgctgctcgc
     4081 cctaaccgtt atactatggg gctacgggta tcgcctgttc tagaaagttc tttgacattg
     4141 cattgctggc gtaaatgtca gcaatgcagc atttcctcac gattctcctt tgacgatcgc
     4201 actttttgct cgttatgatg gcgtcactag cctcagtaaa tccttactcc ggcggtgcgc
     4261 aacccgtgcg cgtgaaccac tatctgaatg ctcatcatgc cgaaatttcg agtttcttta
     4321 tttagcctgg ccctgatgct ggctgtgcct ttggcaccgc aggccgttgc gaaaacggca
     4381 gccgctacca ccgcttcaca accggaaatt gcctccggta gcgcgatgat tgttgatctg
     4441 aataccaaca aagtgatcta ttcgaaccac ccggatctgg tgcgtccgat tgcgtctatc
     4501 agcaaattaa tgaccgcgat ggttgtgctg gatgcacgac tgccgctgga tgaaaaacta
     4561 aaagtggata tcagccagac gccggagatg aaaggggtct attcgcgcgt acgactgaat
     4621 agcgaaatca gccgtaaaga tatgctgttg ctggcgctga tgtcttcaga aaaccgcgcg
     4681 gcggcaagcc ttgcacacca ttatcccggt ggttacaaag cctttattaa ggcaatgaat
     4741 gcgaaagcga aatcgctcgg aatgaacaac acgcgctttg ttgaacctac cggattgtcg
     4801 gtgcataacg tttcaactgc ccgtgactta accaaactgc tcattgccag caaacaatat
     4861 ccgttgatcg ggcagttaag tactacccgg gaagatatgg caaccttctc taacccgacg
     4921 tatacgctgc cgttccgcaa tactaatcat ctggtgtatc gcgataactg gaatattcag
     4981 ttaaccaaaa ccggctttac caatgcggcg ggccattgtc tggtgatgcg tacggttatc
     5041 aataataaac cggtggcgct ggtagtgatg gacgcgtttg gcaaatatac ccattttgcc
     5101 gatgccagcc gcctgcgtac ctggattgaa accggtaaag tgatgcctgt gccggcagca
     5161 gcgttgagct ataaaaaaca aaaagccgcc caaatggcgg cggcggggca gacggcacag
     5221 aacgattaag tgaattcgga tggcgatact ctgccatccg taatttttac tccacttcct
     5281 gccagttttt ccgtttactg gttttaccga tccccggatt catgctgttg gtcggatcgt
     5341 tctcgcgata gaacttctgc aacgtctccg gtgctttata caaatgaccg acgttatgct
     5401 cggcagggta ttgcgcgccg cgctgctgta gcagttccag catctgttct tttaacgcat
     5461 gcacatccac gccttttttc actatgtaat cctgatggaa gacatagcac ataaaatggc
     5521 cgtaatagag cttgtgcacc agctggctgt cgatctccgg cggtaaatgc tcataccact
     5581 cggtgtcgtt acgccgcaga gcgatatcca acgccagaat gtcttcgact tcatcggaat
     5641 gcaccgcctg ataacgaatt gctgcgcccg cagcggcgaa acggtgtaaa aacgctttgc
     5701 tgccttcctc cggcgtacag acaaagaaat cgccttcggc ctgtttgaaa taatccacca
     5761 accacgattt ggcttcgccc acgccatcgc ccgccatttt taacagcaga tgatgctcgt
     5821 atttatcgcg ccagtttttc atgcgcggcg gtaaatggct ggggaacagg tgaccgaatt
     5881 tttgcatcgc acggtcagta aaatgcggac ggaagaattt cactttctcc agcatcgcat
     5941 cggtgcgtcc cttgagatta aagaagaacg gcatcttgtc ggtgcctaac ttatcaatca
     6001 tcaggaaggt gtctttgccg tatttttccg caatatcgta gatatcccgg tgcatatatt
     6061 ccccggcaac cggcagattt tcgaagttag ccagaatatg acggcggatt tcggtcagca
     6121 cttccggctg gttggtgccg atataaaaca cctgctgatt tttttccgct tcgaaggtat
     6181 ctagacgtac cgcaaagacg gccagcttcc cggcgcaacc agaagattca aataaccgat
     6241 ccggatcggc gttataacgt gcgggcgtgt cggcttcaat atcacgaacg cggtggacat
     6301 agtcataatc gtgggcgtga cgaccatcgt gacgcacatc gtcatctttg atgcgatcat
     6361 catccagctt gctaaggatc tgctccggcg tttcgcccag atcaatcccc agatggttca
     6421 ccagcgtcag tttgccgtct tcatttatac gcgcgaataa cgacatttcg gtatacgccg
     6481 ggccacgttg caccagcgag ccgccggagt tgttacaaat accgccgata accgatgcgc
     6541 ctatacacga cgatccaatc accgagtgcg gttcgcgtcc cagcggtttg agggcttttt
     6601 ccagcgaata gagcgtggtg cccggatagg ccagcacctg ttcgcccttg ccaagaacgt
     6661 gcagcttgtc gagacgcagg gtgctgatga tcacgatatc gcgatcatag tcgttaccgt
     6721 ttggcgtcga tccttcggtc aggcctgtat tggcagcctg catcagaata attttgtcgg
     6781 cggtgacaca ggctttcagc acccgccaca attccagtag tgagccaggg aaaacgacag
     6841 ccagcgcgtc gccctgacca gaacggaagc ccttgcgata gcgggccgtt tttgcgggat
     6901 cggtgagcag gtgtgaatga ccgaccagac gggcaagttc attcaaaaag gctttattat
     6961 cagttgttgt catggaagac attttccact ccttgtggtg gcgaaaaaat atcacaaact
     7021 agaatagcgc cttgtatgac aattcagcga gtgaatattt tgaaaattca gacaataaac
     7081 tgagcattct ttttctctct ccatcatgct tatggcaaac tacggttttt gcaccatttt
     7141 cgctgcctcg tcgggcaaca gaggaagaaa aatccatatg aaatggctat gttcagtagg
     7201 aatcgcggtg agtctggccc tgcagccagc actggcggat gatttattcg gcaaccatcc
     7261 attaacgccc gaagcgcggg atgcgttcgt caccgaactg cttaagaaaa tgacagttga
     7321 tgagaaaatt ggtcagctgc gcttaatcag cgtcggcccg gataacccga aagaggcgat
     7381 ccgcgagatg atcaaagacg gtcaggttgg ggcgattttc aacaccgtaa cccgtcagga
     7441 tatccgcgcc atgcaggatc aggtgatgga attaagccgc ctgaaaattc ctcttttctt
     7501 tgcttacgac gtgctgcacg gtcagcgcac ggtgttcccg attagcctcg gtctggcctc
     7561 gtcttttaac ctcgatgcag tgaaaacggt cggacgtgtc tctgcttatg aagcggcaga
     7621 tgatggcctg aatatgacct gggcaccgat ggtcgatgtc tcgcgcgatc cgcgctgggg
     7681 acgtgcttcc gaaggttttg gcgaagatac gtatctcacc tcaacaatgg gtaaaaccat
     7741 ggtggaagcg atgcagggta aaagcccggc agatcgctac tcggtgatga ccagcgtcaa
     7801 acactttgcc gcatacggcg cggtagaagg cggtaaagag tacaacaccg tcgatatgag
     7861 tccgcagcgc ctgtttaatg attatatgcc gccgtacaaa gcggggctgg acgcaggcag
     7921 cggcgcggtg atggtggcgc tgaactcgct gaacggcacg ccagccacct ccgattcttg
     7981 gctgctgaaa gatgttctgc gcgaccagtg gggctttaaa ggcatcaccg tttccgatca
     8041 cggtgcaatc aaagagctga ttaaacatgg cacggcggca gacccggaag atgcggtgcg
     8101 cgtggcgctg aaatccggaa tcaacatgag tatgagcgac gagtattact cgaagtatct
     8161 gcctgggttg atcaaatccg gcaaagtgac gatggaagag ctggacgatg ctgcccgcca
     8221 tgtactgaac gttaaatatg atatgggatt gtttaacgac ccatacagcc atttggggcc
     8281 gaaagagtct gacccggtgg ataccaatgc cgaaagccgc ctgcaccgta aagaagcgcg
     8341 tgaagtggcg cgcgaaagcc tggtgttgct gaaaaaccgt ctcgaaacgt taccgctgaa
     8401 aaaatcggcc accattgcgg tggttgggcc actggcggac agtaaacgtg acgtgatggg
     8461 cagctggtcc gcagccggtg ttgccgatca atccgtgacc gtactgaccg ggatcaaaaa
     8521 tgccgtcggt gaaaacggta aagtgctgta tgccaaaggg gcgaacgtta ccagtgacaa
     8581 aggcattatc gatttcctga atcagtatga agaagcggtc aaagtcgatc cgcgttcgcc
     8641 gcaagagatg attgatgaag cggtgcagac ggcgaaacaa tctgatgtgg tggtggctgt
     8701 agtcggtgaa gcacagggga tggcgcacga agcctccagc cggaccgata tcactattcc
     8761 gcaaagccaa cgtgacttga ttgcggcgct gaaagccacc ggtaaaccgc tggtgctggt
     8821 gctgatgaac gggcgtccgc tggcgctggt gaaagaagat cagcaggctg atgcgattct
     8881 ggaaacctgg tttgcgggga ctgaaggcgg taatgcaatt gccgatgtat tgtttggcga
     8941 ttacaacccg tccggcaagc tgccaatgtc cttcccgcgt tctgtcgggc agatcccggt
     9001 gtactacagc catctgaata ccggtcgccc gtataatgcc gacaagccga acaaatacac
     9061 ttcgcgttat tttgatgaag ctaacggggc gttgtatccg ttcggctatg ggctgagcta
     9121 caccactttc accgtctctg atgtgaaact ttctgcgccg accatgaagc gtgacggcaa
     9181 agtgactgcc agcgtgcagg tgacgaacac cggtaagcgc gagggggcca cggtagtgca
     9241 gatgtacttg caggatgtga cggcttccat gagtcgccct gtgaaacagc tgaaaggctt
     9301 tgagaaaatc accctgaaac cgggcgaaac tcagactgtc agcttcccga tcgatattga
     9361 ggcgctgaag ttctggaatc aacagatgaa atatgacgcc gagcctggca agttcaatgt
     9421 atttatcggc actgattccg cacgcgttaa gaaaggcgag tttgagttgc tgtaatgctt
     9481 tagtaagggc gcgacgtttg cgccctttgt aggccggata aggcgctcgc gccgcatccg
     9541 gcaggaaacg ccggaccgaa tatgcctctt aagtatttat ctgattctgc aactgaatcc
     9601 ttccgctcaa gctaaccccg ccattatcaa ctatgctttt ctcttaattc gctgaaaaca
     9661 acagtaaaag aatgaggaaa gcatcatgcc actcttaaag ctctgggcag gttcactggt
     9721 tatgttggca gccgtgagcc tgccgctaca ggcggcttcc cccgttaaag tcggttcaaa
     9781 aatcgatacc gaaggtgcgc tactcggcaa tatcattttg caggtgctgg aaagccacgg
     9841 cgtaccgacg gtgaataaag tgcaactcgg aacgactcct gtggtgcgcg gggcgattac
     9901 ttccggtgaa ctggatatct atccggaata taccggcaat ggcgctttct tctttaaaga
     9961 tgaaaacgat gcagcgtgga aaaacgcgca gcaaggttac gagaaagtca aaaagctcga
    10021 ttcggagcac aacaagttaa tctggctgac gcccgcgcct gcaaataaca cctggactat
    10081 cgccgtgcgt caggatgtgg cagagaaaaa taaactcact tcgcttgccg acctgagtcg
    10141 ttatctgcaa gagggcggca ccttcaaact ggcagcctcg gcagagttta tcgaacgcgc
    10201 cgatgcgtta cccgcgtttg aaaaagccta tggctttaag ctcggtcagg atcagttgct
    10261 gtcactggct ggtggcgaca cggcggtgac gatcaaagcc gctgcccagc aaacctctgg
    10321 cgttaatgct gcaatggctt acggcactga cggcccggta gcggcgctgg ggctgcaaac
    10381 cttaagcgat ccgcaaggcg tgcaacctat ctatgcgcct gcaccagtgg tgcgtgaatc
    10441 agtgttgaag gagtatccgc aaatggcaca gtggctacag ccagtcttcg ccagcctcga
    10501 tgcaaaaaca ttgcagcaac tgaatgccag cattgcagtt gaaggactgg atgccaaaaa
    10561 agtggctgcc gactacctga aacaaaaagg gtggacgaag taatttcccg tgacttatct
    10621 acgtattaat cctgttctgg cgctgctgct gttgctgacg gtaatcgcag cggcgctgcc
    10681 gtttatcagt tacgcgccta atcgtttagt ttcgggtgag gggcgtcatc tctggcagct
    10741 gtggccgcaa acgatctgga tgctggtggg cgttggttgc gcctggctga cggcctgttt
    10801 tattcccggt aaaaaaggca gcatttgtgc actcattctg gcgcaattcg tcttcgtatt
    10861 gctggtgtgg ggcgcgggaa aggcggcgac acaactggcg caaaatggca gtgcgctggc
    10921 gcgtaccagc ctcggcagtg gtttctggct ggctgaggcg ctggcattgc tggcctgtag
    10981 cgatgccatc cgccgaatct ccacgcatcc gctgtggcgc tggttgttgc atatgcagat
    11041 tgccattatt ccgctgtggt tgctgtactc cggcacgctt aacgatctct cactaatgaa
    11101 agaatacgcc aaccgtcagg atgtgtttga cgacgcgctg gcacaacatc tgacgttgct
    11161 gtttggtgcg gtgctgcctg cgttagtgat tggtgtgccg ttgggcatct ggtgctactt
    11221 ttccactgct cggcaggggg caattttttc tctgctcaat gtcattcaga ccgtgccttc
    11281 ggtggcgctc tttggcctgt tgattgcgcc gcttgccgcg ctggtgacgg cctttccgtg
    11341 gctggggaag ctcggcatag caggaaccgg aatgacaccc gcactgattg cgctggtgct
    11401 ctatgccttg ctgccgctgg tgcgcggcgt ggtagtcggt ttgaaccaga tcccgcgcga
    11461 tgtgctggag agcgccagag cgatgggcat gagcggggcg cagcgattcc tgcatgttca
    11521 gttaccactg gcgttaccgg tatttctgcg cagcctgcgg gtggtgatgg tgcaaactgt
    11581 aggtatggcg gtgattgcgg cgttaatcgg cgcaggcggt tttggtgcgc tggttttcca
    11641 ggggctgcta agcagcgcca ttgatttagt gttgctgggg gtgatcccgg taattgttct
    11701 ggcggtgctg atcgatgccc tgttcgattt gcttatcgca ctgctgaagg tgaaacgtaa
    11761 tgattgaatt tagccatgtc agcaaactgt ttggcgcaca aaaagccgtt aacgatctta
    11821 atctcaattt tcaggaaggg agtttttcgg tgctgattgg cacatctggc tccggcaaat
    11881 ccaccacctt gaaaatgatt aaccgcctgg tggaacatga cagcggagag atccgctttg
    11941 ccggagaaga aattcgctcg ctgccagtac tggagttgcg ccgccggatg ggctatgcca
    12001 ttcaatctat tggcctattc ccccactgga gcgtggcgca aaacatcgct accgtgccgc
    12061 aattacaaaa atggtcgcgg gcacggattg acgatcgtat cgacgaatta atgacgctac
    12121 tggggctgga gtcaaatttg cgtgagcgtt atccgcatca gctttccggt ggtcagcagc
    12181 aacgtgtggg agtggcgcgc gcactggctg ccgatccgca agtcttactg atggatgaac
    12241 cttttggcgc actggacccg gtaacgcgcg gcgcgttgca acaagagatg acgcgcattc
    12301 accgtttgct ggggcgcact attgtgctgg tcactcatga tattgatgag gcgctacggc
    12361 tggctgaaca tctggtattg atggatcacg gtgaagtggt gcagcagggc aatccgctga
    12421 cgatgctgac tcgtccggcg aatgattttg tccgccagtt ttttggacgt agcgaactgg
    12481 gtgtgcgtct gctttcgtta cgtagtgtgg cggattacgt gcgtcgcgaa gaacgggcag
    12541 aaggtgaggc actggcagaa gagatgacgc tacgcgatgc gctctccctg tttgtcgcgc
    12601 ggggttgcga ggtgctgccg gtggtgaaca cgcagggcca gccttgcggt acgctgcatt
    12661 ttcaggatct gctggtggag gcgtaagcgt atgaagatgt tgcgcgatcc gctgttctgg
    12721 ctcattgctt tgtttgtggc actgattttc tggctgcctt acagccagcc gctgtttgct
    12781 gccttgttcc cacaactgcc acgacccgtt tatcagcaag aaagttttgc agctctggca
    12841 ctggctcatt tctggctggt gggaatttcg agtttgtttg cggtgatcat tggcactggt
    12901 gccggaattg ctgtcactcg cccgtggggc gcggaatttc gcccactggt ggaaactatt
    12961 gccgccgttg gacagacttt tccgcctgtc gcagtgctgg cgattgccgt tccggtgatc
    13021 ggctttggtc tgcaaccagc gattatcgcc ttgatccttt acggtgtgct gcccgtcctg
    13081 caggcgacac ttgccgggct gggagcgatc gatgccagcg tgacagaagt tgcgaaaggt
    13141 atgggaatga gtcgtggtca gcgactgcgt aaggtcgagc taccgctggc ggctccggtg
    13201 attctggcgg gcgtgcgaac ttcggtgatt atcaacattg gtacggcgac gatcgcctca
    13261 acggtagggg ccagcacgct gggtacgccc atcatcatcg ggcttagcgg atttaatacc
    13321 gcgtatgtga tccagggggc gttactggtg gcactggcgg cgatcatcgc agaccgcctg
    13381 tttgaaaggc tggtgcaggc gcttagccag cacgcaaaat aaaggtataa cctgcgagca
    13441 tgacgccacc aattccgccg aacgccataa acaggaacag ggcgatgacc ccaattttag
    13501 ctatgcgcat aatgcactcc ttatgttaac gaaaggattg tacagtaaag cgcatttgtt
    13561 aacgaatcat taaatgccga gtgggaaaat atcatggcct tgttcttgcc aactggtgag
    13621 ttgctgctgt tgggcggagg ttcgattttc accgcaccac accagcaatg tacgaccttc
    13681 gaatagttca gggcgtagtt gattgagcga gtgggcgagg acatcaatgc gccatccttg
    13741 ttgactggca atccagccct ccagccacag acgggtggta tcctgaatat tccagccaac
    13801 caccagcgca tctttaccct gttttttacg tgccgaagcc agacaaatgg cgatgtagtt
    13861 gatcagtacg ccgtcgagga tcgccagcag cgcctggaga gtcggttgtt ggcattgaag
    13921 tcgtcggcgc agaggaataa acagatgtgt ggtgagtgtc tgggcggggt aatcctgacc
    13981 gcgctctttg atccacgttc gcaggctatg cagattgccg ctttgcaggt aggtcagtaa
    14041 tgtttcttgc tgatcgcgcc agccgttctg cacatcaaca ttttcattac tgagcagcat
    14101 tttaactttg ctgacctgca cgccgttgtc gatccagcgt ttgatctcgc ggatacggtc
    14161 aatatcggca tcgttgaaca gtcgatgacc gccgtctgtc cgttgcggtt tcagcaaccc
    14221 gtaacgcctc tgccacgcgc gtaacgtgac agggttaata tcacaaagca acgccacttc
    14281 accaattgtg taaagcgcca tcgtctcacc cttgctcgcg aggtcccggt ttaactttag
    14341 acgcagtttt gcgaaccagg tagttttgcc cgttttttgt gcatctatag ggtgatttta
    14401 tttttgccag gcgattttga gtgatcgtac tcacgaattc tcatttttct gcaagagttc
    14461 aaagaaagtt aaacgcaggc aatgtatgtt acgcgtttta aagggaagtg tggtttgcgg
    14521 gtatgtacga ttttaatctg gtgttgctgc tgcttcagca gatgtgcgtt tttttagtca
    14581 ttgcgtggtt aatgagtaaa acgccattat tcataccgtt aatgcaggtc acggttcgtc
    14641 tgccgcataa atttctctgc tacatcgtct tttccatctt ctgcatcatg ggcacctggt
    14701 ttgggttgca cattgacgat tctattgcca atacccgtgc gataggcgcg gtaatgggcg
    14761 gcttactcgg cggtccggtc gtcggtgggc tggttggtct gaccggcggc ttacatcgat
    14821 attcgatggg gggcatgacc gcgctaagtt gcatgatctc aaccatcgtt gaaggattgc
    14881 tcggcggcct ggtacacagc atcctgatcc gtcgcgggcg cactgataaa gtctttaacc
    14941 ccattaccgc cggtgccgtc acgttcgtcg ctgaaatggt gcaaatgctg atcatcctgg
    15001 cgatcgcccg accttatgaa gatgcggtgc gtctggtgag taatattgct gcgccaatga
    15061 tggtcaccaa taccgtcggc gcggcgctgt ttatgcgtat attgctcgat aaacgcgcga
    15121 tgtttgaaaa atacacttcg gctttttctg ccactgcgct gaaagtggca gcctcgacgg
    15181 aaggcatttt gcgacagggg tttaacgaag tgaacagcat gaaagtggct caggtgctgt
    15241 atcaggagct ggatattggt gcagtcgcga ttaccgatcg agagaaattg ctggccttta
    15301 ccggaattgg tgacgaccac catttacccg gcaaaccgat ttcttcgact tacaccttaa
    15361 aagcgattga aaccggtgaa gtggtctacg ctgatggcaa cgaagtacct tatcgttgct
    15421 ctttgcatcc gcaatgcaaa ctggggtcga cgctggtaat tccgttgcgt ggcgaaaatc
    15481 agcgggtgat gggcaccatc aaattgtatg aagccaaaaa ccgtttattc agttcaatca
    15541 accgcacgct gggcgagggg attgcgcaac tgctttcggc gcagatcctc gccgggcaat
    15601 atgagcggca aaaagcgatg ctcacccagt cagagatcaa actgcttcac gcccaggtga
    15661 acccccattt tttgtttaat gcgcttaaca ccattaaagc ggtgatccgt cgcgacagcg
    15721 aacaggccag ccagctggtg cagtatcttt ccactttttt ccgcaaaaac ttaaagcggc
    15781 cttcggagtt tgttactctc gccgacgaaa ttgaacatgt gaacgcttat ctgcaaattg
    15841 aaaaggcgcg cttccagtcg cggttgcagg tcaacattgc tattccgcaa gaattatccc
    15901 agcagcaatt gcccgcgttt accctgcaac cgatagtgga aaacgccatt aaacatggga
    15961 catcacaact gctggataca gggcgagtgg caatcagcgc ccgacgtgag gggcaacatt
    16021 tgatgctgga gatcgaagac aatgccggtt tgtatcaacc ggtaaccaat gccagtgggc
    16081 tggggatgaa tctggtggat aagcgtttac gtgaacggtt tggcgatgac tatgggataa
    16141 gcgtcgcctg tgagcctgat agttacaccc gaataacgtt acgactacca tggagggacg
    16201 aggcatgatt aaagtcttaa ttgtcgatga tgaaccgtta gcacgggaga acctgcgtgt
    16261 atttttgcag gagcagagcg atattgaaat cgttggagag tgttcaaacg ccgtggaagg
    16321 gatcggcgcg gtgcataaac tgcgcccgga tgtgctgttt ctcgatatcc agatgccgcg
    16381 catcagtggt ctggaaatgg tggggatgct cgacccggaa catcgtccgt atattgtttt
    16441 tctcaccgcg tttgacgaat acgctattaa agcctttgaa gaacatgcct ttgattatct
    16501 gctgaagcca attgatgaag cgcgactgga gaaaacgctg gcgcgtttgc gtcaggaacg
    16561 cagcaagcag gatgtttcgc tgttaccgga aaatcaacag gcgctgaaat ttatcccttg
    16621 tacggggcat agtcggattt atttgctgca aatgaaagat gtggcatttg tcagcagtcg
    16681 gatgagcggt gtctacgtta ccagccacga agggaaagag ggctttaccg aattgacatt
    16741 acgtaccctg gaaagtcgta caccactact gcgctgccat cgtcagtatc tggttaacct
    16801 cgcgcattta caggagattc gtctggaaga taacggccag gccgagttga ttttgcgtaa
    16861 tggcttaacc gtgccggtca gccgccgtta tctgaaaagc ttaaaagagg cgattggcct
    16921 gtaaaagact gctaaaatgg ctttttgcct catcaacacc tgaaggcctc atgctaagta
    16981 acgatattct gcgcagcgtg cgctacattt tgaaagccaa taataatgac ctggtgcgta
    17041 ttctggcgct gggtaatgtc gaagccaccg cggaacagat cgccgtctgg ctacgtaaag
    17101 aagacgaaga gggttttcag cgttgtccgg acattgtttt gtcgtcattc ctcaatggcc
    17161 tgatttatga aaaacgcggc aaggatgagt ctgctccggc actggagccg gaacgtcgca
    17221 ttaataacaa catcgtgctg aaaaaattac gcatcgcgtt ttcgctgaaa accgatgaca
    17281 ttctggctat cctcaccgaa cagcagttcc gcgtttcgat gccggaaatt acagcgatga
    17341 tgcgcgcacc ggatcataaa aacttccgcg aatgcggcga tcaattttta cgttattttc
    17401 tgcgtggact ggcagcgcgc cagcatgtga agaaaagcta agacgggtat ggcggccatg
    17461 cgaaacatgg ccgccgacag attatttcac ttctttaaaa ccagcggctt tcatcaccag
    17521 ttccatttgc gccatagtga tacctttttt ggcatcttca gcagaaacgt tgattcctga
    17581 aataccctgc agggctttaa aatccacttt ttccatatcg atagtcacgt tttcctgcgc
    17641 gtaggtatct gtataggtta atttttcttc aacacccgcg atgtttttgt atttggcgct
    17701 taacggctca agtgtcttgg cagcgtcttc tttggtggtt gcaccaatag aagcaaattg
    17761 aattttggtt tcagaagatt gcttaagcac cttgtcacct ttgtagacat aggtaatggc
    17821 aatttcagtg ccgttcagat tggcgctgaa tttcttcgat tcttctttgt caccgcagcc
    17881 agcaagagag aaaaccagaa cagatgcaac aacgagggaa aacagcttat tgaaagcctt
    17941 catgtaaaac tccattttat ttaatcaaga aactggtgac tctcaccagg ggctatatag
    18001 aatatgccta ataccgtgac gtgagcagtc cggaactgga gtagaactct tagtaaaaag
    18061 cactatttca tccttgttgc tgaagcatgg ggaataattg ttcgcaaagt aaaacaccgt
    18121 tattcattgc ttctacccgt gcctcgcttt ctgtattacg aaattgtccc aacacatgtg
    18181 ccagccgata aaaaccgacc gcggagaggt cattcgccag caactctgcc tgactaatag
    18241 cgctctgttc ctgatagcgc cagccgttat ggagcagttg aataagtaac gcctggcagc
    18301 gcatcagcaa ctgatgagcg gtagacggca caggcaaaac gctggcagaa ggtagcggtg
    18361 ccacaggcgc agtttctgcg tccagcgccc aggcgcgggt ttttgtcatc atcacccgtg
    18421 gttccagtgt caattgccca tcaacaaaac tgacaaagcc agaaaccaga ctcacggggt
    18481 cgtctgtttg ttgcaaaagc gccgccatgc gttcaacggc aaaaggagaa caggcagatg
    18541 ccggaaggga taacgtcagg agattatctt caccttcgcc gctgattacc tgcgcatcca
    18601 gcgtctggcg gctgctatcc caaccgagcg aaatacactc agcgaccggc agaataaata
    18661 agttatcgac ctgattaaga ggccgtatgc aggcgggggg acgctggcgt aaatattccc
    18721 gcaaagccac aatgcccggc tggcgtaacg gcgcgctcaa catttgccag gcatcaggcg
    18781 acagcggcac aacgctgctt aagcggttgc gggtagctaa cagcagctcg ccatcggcac
    18841 tgcgttttgc tgcttgtgaa acaatttgcc cacccgccag tgcgccagcc tgaaaactaa
    18901 acagccgacg cgtagctgcc ggtgagtttt cctgttcact tcgcggccaa ctgcgcgaaa
    18961 ggtgcaaaat actgccggtg tcgggatcgg taaaccagat gcgtaaacca taatgctcaa
    19021 tatcctgcca gcaacgcata cctaaagaca ccagccgcag atgatcaagc tttgcttctc
    19081 cggcaatgcc agagccaacg accgtgcgcc acggcacagg aggaacttca ccaacactgt
    19141 cgcgccgggc catctcttgt gcgcaattta atcgactgtt taatgccgca agctgacgta
    19201 agcattctac ggcatgatag tggctggcgc gagtgtggaa ggcatcaacg cttgcccgta
    19261 gctgtcgtag tgattcactc acccagcgcc agttgcaggc ctctgccgcc tgcaatgtgc
    19321 ggttgaatgc tgcctcgtag tgaataagcg gctggctgat gccgccaagc cataatgcct
    19381 ggcttaattg ctgaacatat tgacgacacg tttggccttc ttcgctggca aacggatcgt
    19441 cagatgatgt gacgtgttcg ctgcgcatct gccagattaa atggttaaat tctgcttgct
    19501 gcgctttggc ctcgacgaag gcctgcacag ccagtacgac atgttcgcaa agtgtgcctt
    19561 caatacaatc acaacgggcg aaacgaatac tgctacggga ataaaaacgc acatcgctca
    19621 tcggtaagcg ggcagaggga atttcgcccg gcgcacagaa caactcaatg gtgatgcctt
    19681 tagcgaccag cgcctgcgcg cgtttgcggg tggcatcggg cagggtagcc agttcttcca
    19741 gccagattgc cggatcccac tcttcttctt tttccgtggg ctggacggtg gcacaaagtc
    19801 gttgataact taacaccagc atcacgcgat gacggcacat gccgctggcc ccgcagctgc
    19861 actgagcctc tttcagtgcc tggctgttcg ccagctgggt acggacaccg tcactgaagg
    19921 tggcgattaa agcgccgttc tcatggctga tttccgggac gttgccattt tccagttcct
    19981 taagactgcg cttaacaaaa ccggcattgc ttaacgccgt cagtgcctgc ggtgtcagtt
    20041 ctaataattc cggacgtagt gaattcatga ctgaagattc tccgcaagcc atgatgccag
    20101 ctcgcccggc gtcatggcgg ctatttgtgc gccgacatta accagcgcct gggccgtatc
    20161 gcggtcatag caaggcgttg cggtgctatc gagcgctgcc agtcccagca ctttgatgcc
    20221 gctctggaca cactttttca cctgatgcgt cagcaatgat gatgaacccc cttcgtaaaa
    20281 atcgctcacg aggataatga cgctttttgc tggttgttca ataagttgcc gaccatactc
    20341 cacggcactg gcgatattgg tcccgccgcc caactgtact ttcattaata actctaccgg
    20401 atcggcaacg tctgccgtga gatcaaccac gcttgtgtca aacgccacca gatgggtacg
    20461 aatgccgggt aactgccaca aacaggccgc catcaccgca gagtggatca ccgagtccac
    20521 catcgatccg ctttgatcaa ccagtaagac cagttgccat tgttcgcttt ggcgtttaat
    20581 gcggctgtta aagcgggggg attcgatata caacttgccg tgttgcgggt gccagtgttg
    20641 caggttggcg cgcagagtac ttttgaaatc aaagtttcgc gccagtggaa ttaatgagcg
    20701 gcgacggcga tcgcggacac cagaaaaagc ctgacgaact tcctttgcca gtcgcgccat
    20761 aatttcttca acaacctggc gcactatctg gcgggcggca gccagtactt cgggattcat
    20821 cagatgtttg gtgtgcaaaa cggcgcgtag caggctttcc gaaggctgca tacgttccag
    20881 cacgtcgaga ttcgtcacca catcttcaat gccgtagcgc agtacggcat cgctttccag
    20941 tcgctcaatc acctgttgcg gaaacagagt gtgaatactg ttgatccact caggggtggt
    21001 gagatttgag ccacctaatc caccagagcg ttcaccacgc tggagccgtt caggatcgcg
    21061 cccatacagc cactccagcg cgtggtctat ctgccgggcg ttgtcatcca gcccacaaag
    21121 cgtcgtttct gccgcttcgc caagaattaa tcgccagcgt tgtagctcac gggtggtcag
    21181 aagatcgttc agttcagaca tggaaaactc cccagttttg cagcgatgcc agtgcctgct
    21241 gttcgagttg ttgatgatgt gcaatggcct gtggtggaca atgcaacggc atttgcagcg
    21301 ccgaaacggg aagttgcgcc agttgataat gctcaagcac ctgatgcgcc agcgtcccgc
    21361 gttctcgtgg tggtagccag gccatcgccg cgcgtaaatc gggcagggca ttgataaaat
    21421 cggcatcact tagttgattt aaatgactgc tgaaaccggc gataaatgtc ggctgacagg
    21481 ccagttgatg gcgggccagc gccagcaaac cgtgcagcgc ttcgccggat tgtgccgggg
    21541 ataactgcgc cagcatcgtc agcgccgctt cggcactggc gttaggatgt tccagacgca
    21601 tcagtgcgcc atgagccgcg ccgcgatcca gcgccggggc atgaattgcc tgactgcgtc
    21661 gctccagtaa ggctaccgcc gcagaaagag aaatgccggg gagttgcaca ccgctatgta
    21721 gatcgcgcag gatatgacac aacgcttgcc agctatgcag gtgagcgtga aactcctttt
    21781 catccggtct gccgttagat tcacacagcc acagcgtgcg atcgatagcc gcgcataacg
    21841 tcgtttgtaa tatctgcgcg ccttgcatac cgctaatttc atccagccgc cataaggcat
    21901 atagcacttc cagcgcctgg cccatttcgg caaattgatt ttcctgggcg attaataatg
    21961 tgagttgctc cagtaattgt tgactgaagg acgccagacc cgctaacgcc gcctggctaa
    22021 gacatgtggt gatactgccg attccacccg cgtccagcat atcggcttct aatttatggc
    22081 gtgcggcttc ctgaagtgtt gcaccaaagc atgcggcctc aatcaatgcc gcatgctggc
    22141 taagcgggcg ggttaatttc cagtgttctt caccgttgcc tgcaagtgtc agtgtacttc
    22201 cctgctggcg tacaatccca gggatctcca gtattgccag ccgatgtaac acctgacttt
    22261 gcgccagccc atcgggggta aagcgattta aggtaagctc cgccggaaga gagattgcgg
    22321 tacgttccag ttcgcaggtg acatcttttg gcagaggcgg ttgtggcgtg gaaggggcaa
    22381 gtttaccgaa tccgtcaccc gctaatgtgt ctatcaacgt tagcagaatc ggatcggtat
    22441 cgggatgaat aacgccgcga tagctccacg gcaacggtgc attcagggct tctttaatca
    22501 acgagcctgc tatggcatcc agccagtcag tgcgtaacgg tagtgtatga ccgcgcaact
    22561 gtgccagtgc cattgcatgc agatgtgcgg cagccatatc cgcggtcgaa gcaggcaagt
    22621 tgtgctggcg caaacgggtg agaaccgttt ttagcagttg ttcaccggcc tgctgtaagc
    22681 cccactgcca gcaccagttt tgccagaccg gggcaggcat accggaaagg tatcctgcca
    22741 gcacatcaag gcgcttttca ctgtagggcg tgagataaca acctgtaatg gcatctgcca
    22801 gcgagggcaa ttccggcgtg ttaatgtcct gcgggcattc gcgccacatt tttgccagtg
    22861 ccggagcgtg ccagccaccg cagacgacta acacgtcgcc attattttgc tgcaccgccc
    22921 atgcaatcca gcgagccata aaggcttcgc gctgacgatt gagtgcatcg ccggggaaat
    22981 cgccccgcag ttgggcaaaa tagtgcgcca gcgcagaggg taatgcagtt tgctggcttt
    23041 catcttcgaa caagtgatcc cacagggtat cgctgttatc catgcgggtg gcacgcagca
    23101 gtaaggcctg gctttcatct tgcgtatcag gcgaatcgtc ctcttcttca ctctgcgccc
    23161 agcaaggcaa atcgatgaag taagtttgtg cctgaatacg acgtgcggct tgtagcgcct
    23221 gccactccgg cgaaaattca gcaaatggcg tccaggcacc acgcccgggg gctgcaccgt
    23281 cctgatactg gcaataactg taaatagcta ccggaagctg gtgggaaaaa aaaagttcgt
    23341 ctacccggtc attaaaatca gccgggcctt caatcaacac gtatcgtggc cgctggcttt
    23401 cgattaacga tttcaccagc cgggcgcagg ccggactatg atgccggatg ccgacaatta
    23461 acggctcgct catgcctctt tcctcacggc aggcggtggc gggcttgata ataagcctgc
    23521 cagtgagctt ctttatgtgt ggcgacacgc tgttcgaagt aacggcgcag acgagcgcga
    23581 tcttcttcgt tatctttgac gatggttccg gcaatacagt ccaccaggtc ggcaggctcc
    23641 cccgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg cgtgggccac gttcacggct
    23701 tcagcggtgg acataatcgc tgtcagtgta tccattgacg ttttcttttc gccgttggca
    23761 cgcagatcgc ggaaggtgcg taccagtaac tccagtactg cgtcaggcac tttatgcgga
    23821 ataccgctat gggccagcaa acgcgccgaa gcactggcga ccagttccag ctcctgggcg
    23881 aaatccataa tcggaaagac ggtttcaaaa tcgaagcgac gctttagcgc ggcactcatc
    23941 tcattgacgc cgcgatcgcg ggtattggca gtggcaataa tattaaagcc ttcccgcgca
    24001 tagagctgac tggcttcacc ggtgagttcc ggcaccgtca tcaccctgtc agaaagcata
    24061 cctaacagac agtcttgtac ttccagcggt gtacgcgtta tctcttcaaa acggacgatt
    24121 ttgccgtcgc gcattccctg gtaaagcggg gcagggacca gtgcttccgt tgacggacca
    24181 tggttgataa gcagggcgta gttccagccg tacttgattt gatcttcggt agtagatgcg
    24241 ccgccctgga tggttaaccc ggcatcgccg ctaatagcgg ttgccagtaa ttcagacagg
    24301 agagattttg cggtgccagg ttcccccacc aacatcaaac cgcgcccggt ggcaagagtg
    24361 actaacatgc gttcgacagc ggagggacta atcaccactt tacggctaat attttgcgcg
    24421 ctatcgccaa ggataaaagc acgggccgca ggcaaactta actgccaacc gggcgggcaa
    24481 ggtgcgttat cattttgttt taattttgcc agttcatcgg cgtataacac agcagcaggc
    24541 ggacgctgca gatgattatt ctgcggtgac atatattcat ccctgaaatg tattaat
//
LOCUS       DAZFRN010000069        24568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFRN010000069 DAZFRN010000000
VERSION     DAZFRN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.069"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..2238
                     /locus_tag="ACHKUB_002638"
     CDS             <1..2238
                     /locus_tag="ACHKUB_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO6655639.1"
                     /translation="IEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNR
                     NRPMTSVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGD
                     TRELLDILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPV
                     IDVEFSHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMN
                     SKADEDVRKELNAIADVSTGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPAS
                     RVYPGSSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATE
                     GATDLWEGSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQ
                     SIQSLQANITSLLADIRKIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELND
                     YFRTGKRKEQQMLEEEDAEQRRSRSGLWGKISQWSGINNPGWEDYRKRDFDPENPEIT
                     LNNRREAIEYIEEIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGR
                     INARLEDEGFTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQSGVWGTVCR
                     WFGTSDLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEID
                     AFFCAFREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREE
                     LETML"
     gene            2235..3140
                     /locus_tag="ACHKUB_002639"
     CDS             2235..3140
                     /locus_tag="ACHKUB_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO6655640.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAR
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            3137..4207
                     /locus_tag="ACHKUB_002640"
     CDS             3137..4207
                     /locus_tag="ACHKUB_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO6655641.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLLPAMNLCQLPG
                     RAGALLTTLLHHISTINRFDNLMTQPLLSDGPLTVLMDHYLDTDDLADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGTGTTKNSVFQHIQSLPHGQQKEALLASAALPLLFRPREVQG
                     KMYGDGGMGGWQNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRRAFPDTTILEIRPR
                     KKLKQTGEEGKSGGLLSFTSAHIDTWRQQGYEDTMLAMEHIRKPLEARQALTRSEAVL
                     QKSLEKTEEADLALRNAMARIK"
     gene            4336..4803
                     /locus_tag="ACHKUB_002641"
     CDS             4336..4803
                     /locus_tag="ACHKUB_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655642.1"
                     /translation="MRLSNIFLAVVLTVTVSVAHAIPNMWGSGFTMGVTEFSIDNGKW
                     GINISCTGNPNENNVLAHTIYIYKGDKVVANSEEKNISFVIDGEEFWGVVPDGTRMND
                     NAWVSFVKAISTATGFELYINEKKVATFNPSAKNVKKVMDNNFIQSCWNTWYE"
     gene            4859..5488
                     /locus_tag="ACHKUB_002642"
     CDS             4859..5488
                     /locus_tag="ACHKUB_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655643.1"
                     /translation="MKHFLILVIGLLISITATAESLGQFEEDITGFPQGVWFYKEKAG
                     WKNETGYKKEYLNSLLYKAEEIANYPGNRYTIYRSKNSSIIFVADNETRTFYLLNNKD
                     ALWSDIQIGYWAENKNSCHYVFVDTQYLAQSNELDEFFRTGVLDTHGVGESDRKVKEI
                     RQKHKTHVLLRLPAQCVNDAILSRMNKSERRDLMKAQEDFKQYLARMEL"
     gene            5923..6375
                     /locus_tag="ACHKUB_002643"
     CDS             5923..6375
                     /locus_tag="ACHKUB_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655644.1"
                     /translation="MKHRHILTFFVAFLFMTIVPDSDAALVKRPEKGRAPFPEYVEFA
                     QNRNQFPCNARLRFKGLCFVEQTDPWGAASVVRIPFNSPNGKGRLNAYGVVVHAIMVG
                     DVVVGGSYSTGKNVTWNFDTFYREGKRRGKVTFYVDGKYWHYRDYPLE"
     gene            6493..6726
                     /locus_tag="ACHKUB_002644"
     CDS             6493..6726
                     /locus_tag="ACHKUB_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6655645.1"
                     /translation="MRGCTLLLFSEAPFLREQEVAVITAYRNVFIQDDPGMHFRWVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            6826..7647
                     /locus_tag="ACHKUB_002645"
     CDS             6826..7647
                     /locus_tag="ACHKUB_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6655646.1"
                     /translation="MRLASCFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQQEGFQPFFACQTCVRDPGRRGYTKHMLRLRRNGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEKRDAMQSLLLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGQDLW
                     SAYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            7647..7892
                     /locus_tag="ACHKUB_002646"
     CDS             7647..7892
                     /locus_tag="ACHKUB_002646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655647.1"
                     /translation="MKHIPEGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLFYRPG
                     EISLCRLSTGNGIWPSVCDRSGYAYGCPEFQPNIIHP"
     gene            7986..8459
                     /locus_tag="ACHKUB_002647"
     CDS             7986..8459
                     /locus_tag="ACHKUB_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6655648.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDDDDETWILFSAMNGN
                     RAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            8475..8951
                     /locus_tag="ACHKUB_002648"
     CDS             8475..8951
                     /locus_tag="ACHKUB_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6655649.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRGLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            9020..9241
                     /locus_tag="ACHKUB_002649"
     CDS             9020..9241
                     /locus_tag="ACHKUB_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6655650.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            9260..9904
                     /locus_tag="ACHKUB_002650"
     CDS             9260..9904
                     /locus_tag="ACHKUB_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFO6655651.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQALT
                     ADAEAAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEAIHINAYTFTEAGLHAG"
     gene            9954..10328
                     /locus_tag="ACHKUB_002651"
     CDS             9954..10328
                     /locus_tag="ACHKUB_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6655652.1"
                     /translation="MSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            10375..10752
                     /locus_tag="ACHKUB_002652"
     CDS             10375..10752
                     /locus_tag="ACHKUB_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6655653.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRSNYRMVNDIIRGEHSEAKR"
     gene            10749..11241
                     /locus_tag="ACHKUB_002653"
                     /pseudo
     CDS             10749..11241
                     /locus_tag="ACHKUB_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            complement(11320..12308)
                     /locus_tag="ACHKUB_002654"
                     /pseudo
     CDS             complement(11320..12308)
                     /locus_tag="ACHKUB_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(12456..14049)
                     /locus_tag="ACHKUB_002655"
                     /pseudo
     CDS             complement(12456..14049)
                     /locus_tag="ACHKUB_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(14080..14430)
                     /gene="tnpB"
                     /locus_tag="ACHKUB_002656"
     CDS             complement(14080..14430)
                     /gene="tnpB"
                     /locus_tag="ACHKUB_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6655654.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDETPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTFRLNSLTML"
     gene            complement(14427..14718)
                     /locus_tag="ACHKUB_002657"
                     /pseudo
     CDS             complement(14427..14718)
                     /locus_tag="ACHKUB_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14760..14954
                     /locus_tag="ACHKUB_002658"
     CDS             14760..14954
                     /locus_tag="ACHKUB_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6655655.1"
                     /translation="MQGSGVTDGIGFTLTNNIDCESGIIFDNDEDKTDSAVLFPWIER
                     ACQDVRELCQFQRLQKTRTD"
     gene            15036..15893
                     /locus_tag="ACHKUB_002659"
                     /pseudo
     CDS             15036..15893
                     /locus_tag="ACHKUB_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(16014..16090)
                     /locus_tag="ACHKUB_002660"
     tRNA            complement(16014..16090)
                     /locus_tag="ACHKUB_002660"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16054..16056),aa:Asp,seq:gtc)
     gene            complement(16223..16954)
                     /gene="dnaQ"
                     /locus_tag="ACHKUB_002661"
     CDS             complement(16223..16954)
                     /gene="dnaQ"
                     /locus_tag="ACHKUB_002661"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO6655656.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            17019..17486
                     /gene="rnhA"
                     /locus_tag="ACHKUB_002662"
     CDS             17019..17486
                     /gene="rnhA"
                     /locus_tag="ACHKUB_002662"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO6655657.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITKWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(17483..18205)
                     /gene="yafS"
                     /locus_tag="ACHKUB_002663"
     CDS             complement(17483..18205)
                     /gene="yafS"
                     /locus_tag="ACHKUB_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6655658.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            18239..18994
                     /gene="gloB"
                     /locus_tag="ACHKUB_002664"
     CDS             18239..18994
                     /gene="gloB"
                     /locus_tag="ACHKUB_002664"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO6655659.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDSETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            19066..20424
                     /gene="mltD"
                     /locus_tag="ACHKUB_002665"
     CDS             19066..20424
                     /gene="mltD"
                     /locus_tag="ACHKUB_002665"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO6655660.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(20473..21243)
                     /locus_tag="ACHKUB_002666"
     CDS             complement(20473..21243)
                     /locus_tag="ACHKUB_002666"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6655661.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKLGGKLIVMDVMSPGHPVRDIWL
                     QTVEALRDISHTRNYASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(21321..22121)
                     /gene="yafD"
                     /locus_tag="ACHKUB_002667"
     CDS             complement(21321..22121)
                     /gene="yafD"
                     /locus_tag="ACHKUB_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6655662.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            22362..23276
                     /gene="yafC"
                     /locus_tag="ACHKUB_002668"
     CDS             22362..23276
                     /gene="yafC"
                     /locus_tag="ACHKUB_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO6655663.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETLDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAHEGAVREA"
     gene            complement(23273..24076)
                     /gene="dkgB"
                     /locus_tag="ACHKUB_002669"
     CDS             complement(23273..24076)
                     /gene="dkgB"
                     /locus_tag="ACHKUB_002669"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO6655664.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVARAKQHGIHITSYMTLAYGKSLKDEVIARIAAKHNATPAQVILAWAMGEDYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNVRLVSPEGLAPEWD"
     gene            complement(24239..24315)
                     /locus_tag="ACHKUB_002670"
     tRNA            complement(24239..24315)
                     /locus_tag="ACHKUB_002670"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24279..24281),aa:Asp,seq:gtc)
     gene            complement(24370..24485)
                     /gene="rrf"
                     /locus_tag="ACHKUB_002671"
     rRNA            complement(24370..24485)
                     /gene="rrf"
                     /locus_tag="ACHKUB_002671"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6215 a         6150 c         6296 g         5907 t
ORIGIN      
        1 attgaggagc tggagggcga acaaatcaaa accgcccgca gggagatggt gctggctgtt
       61 gtcggcacga tgaaagcagg caaatcaacc accatcaacg ccattgtggg gcaggaaatt
      121 ctgcctaacc gtaaccgccc catgacctct gtaccgacgc tcatccgcca cgttcccgga
      181 aaaactgagc cggttctcca tctggaacat attcagcctg tccgcaattt attaatcaca
      241 ctgcaggaaa aactcgccac cccggcagga cagcaggtcg cacagaccct gcagcaaacc
      301 ggggataccc gcgaactgct ggatattctg acggatgatg gctggctcaa aaatgaatac
      361 cacggggagg aggaaatctt taccggactg gcatcgttaa acgatctggt tcgtcttgct
      421 gcggcaatgg ggactgaatt tccttttgat gaatacgcag aagtgcagaa actgccggtg
      481 atcgacgtgg aattcagcca tctggtgggg atggatgcat gccagggaac actcacactg
      541 ctggataccc ccggccctaa tgaggccgga caaccgcaga tggaagtgat gatgcgggat
      601 caactgcaga aagcctctgc ggttctggct gtgatggatt acacccagat gaactcaaaa
      661 gcggatgaag acgtccgtaa agagcttaat gccattgctg acgtatcaac cggccgcctg
      721 tttgtactgg tcaataaatt tgatgagaaa gaccgcaatg gcgatggggc agatgccgta
      781 cgccagaaag ttccggcaat gctgaacagc gatgtgctgc ccgcctcccg cgtttatccc
      841 ggatcctcac gccaggcata cctggctaac cgtgcgcttc atgagttacg gaaaaacgga
      901 acccttcctg ttgatgaagc ctgggtcgat gattttgtca gggaagcctt cggtcgcatg
      961 aaaaaagatt acgtctgtaa agacagcgaa ctggcaactg aaggggcgac agacctgtgg
     1021 gaaggctcac ttatcgatca actgataacg gaagtcatac tgagttcaca ctccagagcg
     1081 gcggcactgg cggttgattc tgctgccgca aaactgatgc agaatgcaga aaatgtcagc
     1141 gaatatctgt cattacgcca tcaggggctg cagcaaagca ttcagtcact gcaggcgaat
     1201 atcaccagcc tgcttgcgga tatccggaaa atcgcggact gtcaggagca ggtgaccgct
     1261 gatgtcagaa tggccatgga ggagatcgat gccagaaccc gggaattact gacgggggtc
     1321 tgcacctcac tggaagagga gctgaatgac tatttcagaa ctggtaaacg caaagaacag
     1381 caaatgttgg aggaagaaga cgcagaacaa cgaaggtccc ggtccggatt atggggaaag
     1441 atttctcaat ggtccggtat caacaacccg ggctgggaag attacaggaa acgagacttt
     1501 gacccggaaa accccgagat aacactaaat aatcgcaggg aagcaattga atatatagag
     1561 gaaattgagt caactgtgac cagcctgcac cgtgaggctg aagcacagtt ccggcctgag
     1621 ctggagaaaa tcgtcagcgg gattgaaaca ggttttcgtg gcacggccct gtacgccaca
     1681 gaaaacattg ccggtcgcat caatgcccgc ctggaggatg agggctttac cgtaaaaatc
     1741 agttttccgg cagtcagtca gttacagacc cggctcgcgg taaaaacaaa tctgagtgcg
     1801 cttatggagg aaagaacgga gaccgtcacc cgtcgccgtc ggcagagtgg cgtatgggga
     1861 accgtttgtc gatggtttgg caccagtgac ctgggctggg aaaactatga cgaggatgtg
     1921 agtcgcagcg tgatcaatat caacaaggtc agagaggaag ttatgtcact gacccgggca
     1981 tatttcgggg agctgcaggc atccattgag caggatatta accagcccgt ccgccaggaa
     2041 atcgatgcct ttttctgcgc attcagggag aaagttgaac aactgcgtaa cacgctgatt
     2101 cagagctctg aagatcataa acgcgatcag caggcacagg aacggcttac cgggagactt
     2161 caggcattaa atgaaagggt tcctgagctc attactgaca gtaaggcgct gagggaagag
     2221 ctggagacaa tgctgtgact tcaccattta ttcaacaaat tgctgataac cgggtatgtc
     2281 aggtactcac ctgtcttcct gaaaaatttg tggttgattt cgcaaacgga atcgatgttg
     2341 cacaggagca tatccgcacg gccagagagc gcacgttctt ccggcgttta aaagagggac
     2401 tgaccggcga aggcgctgca cggcagaatg ccatcaatgc ttcgcttgca cagggcgttg
     2461 aggcgtccct gcgctggctg acggagctga caacgtcact tgccaccaca aactacgcga
     2521 ttacccgggt aaacgacagg gtcagttcac tggtcagtga tactgccagg ctggcgcatt
     2581 attccgcaga tacacgggag cagttactca tcctggccga tcaggttcac cataaactga
     2641 atcatcttga agaaaaactc caccgtgttg accaggttca gcgggcgcag ttacatcttg
     2701 agcagatatt ctcatggtgg agcgccgggc gatacgcgtc attttcccct gccggacgtt
     2761 gttatgtggc acttgaagag cttcgctggg gcgcgtttgg tgatgtgata cgtcagggcg
     2821 aaacaggcca ggttaaccag ctactggata ttctcagaca taaagcatta acgcagatgg
     2881 cacaggagag tggcggtagt gcaaccgtgc gtctgaacac tctggactgg cttggtggtc
     2941 agggccggga gcaggcagat aacgagtggc atgacgcgat taactggctg ggagactggt
     3001 gcagtgaaga gcagcatccg gtgatctggt caaccacgca ggctgcagaa cacttaccgg
     3061 ttcgcatgcc tcgcctctgc tctgcagaac gcctctctga aagtatggtc gatgaaatat
     3121 ttcagaaggg ggctgcatga gtacagaaat gaaaacgggg ctggtgctgt ccggtggcgg
     3181 ggcggtgggc gcttatcagg cgggagtggt taaggcactg gcagagtgtg gtacacagat
     3241 cagcatggtt tcaggggcca gcattggcgc attcaatggt gccattatcg cggcctctcc
     3301 cgatctgtca gaagctgccg tacgcctgga ggcgctctgg gatcatctgg ggaataatca
     3361 ggtgctgtcg gtaaacagat tggtttactt ttcattgctg aaaaaactgc tcccggccat
     3421 gaacctctgc cagctccccg gacgtgcggg cgcactgctt acgacgcttc ttcaccatat
     3481 atcgacaatc aacaggtttg acaatctgat gactcagccg ttgttgtcag atgggcccct
     3541 gacagtgctg atggatcatt atcttgatac tgatgatctg gctgacgggc taccgttgta
     3601 tgtgtcgctg taccccacgg aagggggaat gcaggatatt atcgactgca ttcgtgctga
     3661 actgggtacc ggaactacaa aaaactccgt ttttcagcat atccagagcc tgccccacgg
     3721 gcagcagaaa gaagccttac tggcgtcagc agcactgccc ctgctgttcc gtccccgtga
     3781 ggtacaggga aaaatgtacg gtgatggtgg tatgggcgga tggcaaaata tgcaggggaa
     3841 tacccctgtg acgccactgg tggatgccgg atgcaatatg gtgattgtgt cgcatctgag
     3901 tgacggttct ttatgggatc gccgggcttt tccggacacc acaatccttg aaatccgtcc
     3961 ccggaaaaag ctgaaacaaa caggggaaga aggcaaaagt ggcggtctgc tcagttttac
     4021 atcggcacat atcgacacct ggcgtcagca gggctatgaa gacacgatgc tggcgatgga
     4081 gcatatccgg aaaccactgg aggcgcgtca ggcactgacc cggtcagagg cggtgttgca
     4141 gaaaagtctg gagaaaacgg aagaggcaga tttggcactg agaaacgcga tggcccggat
     4201 taaataaaga cgccccaaag aaacgccacc gcctgtggtg gtgtttttcc tggctggtgt
     4261 tattgaaaat attttcagat atatattacc cggacgatgg ttaagattga tttatttaat
     4321 tttggggaga aaattatgag actgtcaaat attttcctgg ctgtagtttt aactgtgacc
     4381 gtatctgtag cacacgctat tccaaatatg tggggcagtg gcttcacgat gggcgttact
     4441 gagttcagta ttgataacgg taagtggggt atcaatatca gttgcacagg taaccccaac
     4501 gaaaacaatg tgctggcgca cacaatctat atttacaaag gagataaggt ggtcgcaaac
     4561 tcggaagaaa aaaatattag tttcgttatt gacggtgaag aattctgggg tgtggttcct
     4621 gacggtaccc gtatgaacga taacgcctgg gttagctttg taaaagcaat aagcactgca
     4681 accgggtttg aactgtacat aaacgagaag aaagttgcga cgtttaaccc gtctgccaaa
     4741 aacgttaaga aagtgatgga taataacttt atccagtcgt gctggaatac ctggtacgaa
     4801 taacgattac aggagagggg gacttcctgg cccccgattt atttaaggag tgcatgaaat
     4861 gaaacatttt ttaattctgg ttatcggttt actcatttcc atcaccgcga ctgccgaatc
     4921 gctgggtcag tttgaagagg atatcaccgg ttttccgcag ggagtatggt tttacaaaga
     4981 aaaagcaggc tggaaaaacg aaaccgggta taaaaaagaa tatttaaaca gtctcctcta
     5041 taaggccgaa gaaattgcga attatccggg taacagatac acgatttacc gtagtaaaaa
     5101 cagcagcata attttcgtcg ctgacaacga aactaggact ttctacttgc taaacaacaa
     5161 ggacgcctta tggtctgata ttcagatcgg gtattgggct gagaacaaaa actcgtgtca
     5221 ctacgtcttt gtcgatactc aataccttgc gcaaagtaac gaacttgacg agttctttcg
     5281 aaccggggtg ctcgatactc atggtgttgg tgaatcggat cgtaaagtaa aagagatcag
     5341 acaaaaacat aagactcatg tcttactccg cctgcctgcg caatgtgtga atgatgcgat
     5401 cctcagcaga atgaataagt ccgaacgtcg tgatctaatg aaagcgcaag aagatttcaa
     5461 acaatatctt gccagaatgg agctgtaagt attcaacctt ttcgcaactg tgagtgaaat
     5521 gaaaatcaaa actatacttt atactatcat tcttttctgt gaaattggca tcaccgacac
     5581 ttatgcactc ggtggttcgg atatccgtca ctgcgaacca actgaagaat ctgtaattat
     5641 taacccggac gagtcaccgg tgttaatata tagcttcagt aaacacgaag tgtataaact
     5701 gatgcagggg gatacctgca ctgggctagt tattagcgat aaaaagccaa agcctgtcca
     5761 cgtttaatgg acatcgcttg cgaaccgcca gaagtcatgt ctgccagcat tcatctactg
     5821 gccgcggata attatacgac catggatttc tgccataacg ccgaattaat cggtcgtatt
     5881 ccgtaagccg tggtatcgaa taatttaaga aggattttta tcatgaaaca ccgacacatt
     5941 ttaacgttct ttgttgcttt cctgttcatg actatcgtac ctgatagcga cgccgcatta
     6001 gtaaagcgtc ctgagaaagg tcgtgcaccg ttcccggaat atgtggagtt cgcgcaaaac
     6061 cgtaaccagt tcccatgtaa tgcacgttta cgctttaaag gtctctgctt tgttgaacaa
     6121 actgacccct ggggtgcagc aagcgttgtc cgaatccctt tcaattcccc caatggtaaa
     6181 ggcagactta acgcttatgg cgtggttgtt catgcaataa tggttggtga tgttgtcgtt
     6241 ggtggcagct attcaactgg caagaatgtt acctggaatt tcgatacgtt ttatagggag
     6301 ggtaaacgtc gcggtaaggt gaccttttat gttgatggta aatactggca ctatcgcgac
     6361 tatccactcg aataatccat aacgaacaaa actattaaag gaattatgcg tcttatctgc
     6421 gcattattaa aatcaatagt aaattcgctc ctgccattat ggctggggct ttttattttc
     6481 aggtgaaaac acatgagggg ctgtacatta ctcttatttt ccgaagcccc tttcttacgc
     6541 gaacaggagg ttgctgtcat aacagcttac cgcaatgtgt ttatccagga tgacccggga
     6601 atgcatttcc gttgggttat ccgcaacgcg gaaggtcagc gacgctggcg ctgccggaat
     6661 tctgaacccg atgccggaaa ggtgcttaat acgcggctcg ccagtgacgg tcttctcaga
     6721 caataaacgt cattcatttc acccatcagg ccgcgtcttc tccgggagac gtggcttttt
     6781 catttattac accagtaaat cttaaccacc gataaggagc aaagtatgcg attagccagt
     6841 tgttttggtt atgctgcgaa ccagatacgc cgtgaccgtc cactgacaca tgaagagctg
     6901 atgcaccatg tgccggggat tttcggggaa gagaaacaca cgtcccgaag ccagaactat
     6961 acgtacatcc ccaccatcac cgtactggaa agcctgcagc aggaaggttt tcagccattc
     7021 ttcgcctgcc agacctgtgt gcgcgacccg ggccgccggg gatacacaaa acacatgctg
     7081 cgcctgcgcc gtaacggaga gataaacgga gaacatgtcc ctgaaattat tctgctcaac
     7141 tctcatgacg gtacctccag ctaccagatg ctgccgggtt acttcaggtt cgtctgccag
     7201 aacggatgcg tctgtggtca gtctctgggg gaagtgcgtg ttccgcaccg gggaaatgtg
     7261 gtggacagag tcattgaagg ggcttacgag gtggtgggcg tgtttgaccg gatagaggag
     7321 aagcgtgatg ccatgcagtc tctgctctta ccgccaccgg cacgccaggc gctggcacag
     7381 gcggcactga cttaccgtta tggtgatgaa catcagcccg tcaccactgc cgacattctg
     7441 acaccgcgac gccgggagga ttacggtcag gacctgtgga gtgcttatca gaccatccag
     7501 gagaatatgc tgaaaggcgg gatttccggc cgcagtgcca gaggaaaacg tatccatacc
     7561 cgggccattc acagcattga caccgacatt aagctcaacc gggcattgtg ggtgatggca
     7621 gaaacgctgc tggagagtat gcgctgatga aacatatccc tgaaggcgtg attcaggcac
     7681 cagaatcacc cgacgccccg gaggcgctgc cggggtgtcg ttccatttct gatgatgacc
     7741 tgtgcgcctg gtgtacccgt ctgttttacc gtcccggaga aatcagtctg tgcagactga
     7801 gtacaggcaa tggcatctgg ccatccgtct gtgaccggag cggttacgcg tacggctgtc
     7861 cggaatttca gccgaatatc attcatccct gatattaccg ggtctcatgg tcagtgatga
     7921 acgtgatgtt gccagctccc gacctttact ccctctcatt tattcacatt acaaggattt
     7981 catacatgaa aacattgtct cagaacacca cctcttcggc ctgtgcaccg gagactggcc
     8041 tgcaacaact ggttgccacc atagtccctg atgaacagcg catcagcttc tggccgcagc
     8101 attttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc tggatggacc
     8161 gtctttgcga aaactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     8221 catttatggc ccccgaaccg gatgatgatg atgacgaaac atggatactg ttcagtgcca
     8281 tgaacggtaa ccgcgctgaa atgagcccgg aagctgccgg tattgccgcc tgtctgatgg
     8341 catacagcca tcatgcctgc cgtacggaat gttatgccat gacggtccat tattaccggt
     8401 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     8461 tggaggcagg gacaatgcag cagctttcct ttctgcccgg agagatgacg cccggtgagc
     8521 gcagcctcat tcagcgggcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     8581 ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg ggactggagc
     8641 gtgaagagtt ccgggggctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     8701 tctttaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt aaacgcgccc
     8761 tgtatcacaa tgccgctgct gtggtgctgg cacacaatca cccgtccggt gaagtcacac
     8821 ccagcaaagc agaccgcctt atcacggaac gtctggtaca ggcactgggc ctggtggata
     8881 tccgggtgcc ggaccatctg atagttggtg gcaaccaggt tttctccttt gcggaacacg
     8941 gcctgcttta atccgtcacc gccacaatca catcacgcct gttttcactt ttatcttctg
     9001 tcttcagagg tatcccatca tgaaaattat cacccgtggt gaagccatgc gtattcacca
     9061 acagcatcct gcatcccgtc tttttccgtt ctgtaccggt aagtaccgct ggcacggcag
     9121 cgctgaagcg tataccggtc gtgaagtgca ggatattccc ggtgtgctgg ctgtatttgc
     9181 agaacgccgt aaggacagtt ttggcccgta tgtccggctg atgagcgtca ctctgaactg
     9241 agtcagtaag ggcattctga tgagcagaat tatcaccacc accgtgtata cactgcatga
     9301 gctatcctcc accgcacagg aaaaagcccg cgactggtac cgtcagcatc atgctgacag
     9361 caactggtat gaaaacgtgt atgaggactt cagggaggtc tgtggcattt tcggaattga
     9421 cctccgccag cgcgttttcc ggctcagcaa cggcaggttt atggaagagc cgtgcatctg
     9481 gttcagcgga ttctgcagcc agggggacgg agcctgtttt gaagggcgct ggcactggca
     9541 gccagcgaca ccccggaaaa tcagggaata tgcccctcag gaccgtgagc ttcatcgtat
     9601 cgcggatgcc ctgcaggcgg tacagaagcg taacttctgg cagttacagg ctgaaatcag
     9661 tcaccgggga cgttactgcc atccgtacag catggatatc accgtcaccc ggaacagtcc
     9721 gactggtcag gcactgacag ccgatgcgga agccgctgtc agcgaagccc tgcgcgacct
     9781 ggccttctgg ctgtaccgcc agctggaaaa cgagtacgac tggctgacct cagacgccgc
     9841 tgtagatgag gccattcaca tcaatgcata taccttcacc gaagccggtc tccacgccgg
     9901 ctgacctgtc atcgttaaac tatcattcac cgattttctg tgaggacttt atcgtgtcag
     9961 acacgctcca tgaaacaaat tatcccgacg acaacaacga ccgcccctgg tgggggctac
    10021 cctgcaccgt gacgccctgt ttcggggcac gtctggtgca ggagggcaat cagttgcatt
    10081 accttgccga ccgcgccggt atcagagggc agttcagcga cgcggatgca taccatctgg
    10141 accaggcttt tccactgctg atgaaacaac tggaactcat gctgaccagc ggtgaactga
    10201 atccccgcca tcagcatacc gtcacgctgt atgccagagg gctgacctgc gaagccgaca
    10261 ccctcggcag ctgtggttac gtttatatgg ctgtttatcc gacactggca cccgcaacca
    10321 cctcataacc ccgaatatct cacctttagc actaccagtt acagagaact taagatgaaa
    10381 acattacctg acacacacgt acgggaggca tcgcgctgcc cgtctcccgt caccatctgg
    10441 cagacactgc tcacccaact gttggaccag cattacggcc tcacactgaa tgacacaccg
    10501 ttcgctgatg aacgtgtgat tgagcagcat attgaagcag gcatttcact gtgcgatgcg
    10561 gtgaactttc tcgttgaaaa atacgcactg gtacgtaccg accagccagg attcagcgct
    10621 tgtacccgtt ctcagttaat aaacagtatt gatatcctcc gggcccgccg ggcaaccggc
    10681 ctgatgaccc gcagcaacta cagaatggtg aacgacatca tccggggtga acattcggag
    10741 gcgaaacgat gaaactgact ctgacgctcg aagcagacag cgttaacgtg caggcactga
    10801 acatggggcg aattgtcgtt gacgtcgatg gtattgaccc cgctgagttg aataataagg
    10861 tcgctgaaaa cggttattca ctccgcgtgg tggatgctcc cgaacagcag acaaccgact
    10921 ctccaccacc gtttactgcc cttaccgaca tacgttcgtt gcagtaccgc acatatcacg
    10981 gaaaaggaca acgcctggct gtactcactg tcacatcaga cgaatgactt cggcgaatca
    11041 gaatggattc attttacagg taccggttat ctgttacgta ctgatgcgtg gtcgtatccg
    11101 gttctgcggc ttaaacgtct tggcctgtca aaaacgttcc gtcgtctggt tgtcactctc
    11161 atccggtgtt atggcgtcgg tctcattcat ctggctgcca gtgcaggatg tctgccgggc
    11221 ctgcccactt tcgactggta actaggaacg acatgacatc attaatcacg gaaacggcgc
    11281 tggaaattct gattgcctaa atagctgcgc ttaataccgc tacacttttg ccagcccatg
    11341 tttgcctccg gggaatgggc taacggtttc cataaaatgg cgaacttttt tcaacagttg
    11401 ccacattgag cggcactgat gattacgcgt tattgtgtcg tgaagtgcct gccatagccg
    11461 ttcaacatga ttcacccatg gcgagtaaac cggctgataa atgaccctga acttcgggtt
    11521 ctccttcagc cagctctgtg tttcccggct tttgtggata atgtagttgt ccacgatcag
    11581 cgtgatggtt ttcgcccgac ggtatgtcgc tttaagccgt ttcagcaggc tgatgaacag
    11641 cgccgaactt ttgctgttgc cgcccacata gctggcttta cctgtcccgg cgtgaccacc
    11701 cgtttttgct gtccgcgcag ttgccagtcc gcaccgattt tgggattaag atggatatcc
    11761 acttcatctt cataaaagac cggatgctct gcgcagcatt cgtccagtgc tttatggatt
    11821 gctgccatct tttcatcttt atgcgggtca cggatacgca gagttggcgc agcccttcgc
    11881 cacacaatcc ccgcagacgg caaccagcgg cgaacggttc cggcatttaa ctggcaaccg
    11941 gttatctcat tgatttttat tgccagcagt tctgtactcc agcgtgaacg ctggtagcca
    12001 aagtcgccgg gagaatgttt taccagctca cgtaacagtg tgcagatatg ctcaaacggc
    12061 cagcgacggg cacgcccggc aggtaatgat ttcagtccct caacacccga ctgcgtgaac
    12121 cagttaatcc agcgtccaac agaggaacgg gcgcagcaga gcgttctggc aacgtcgctg
    12181 acacggtcgc cccggtgcag catcagcatg gcagtcagtc tgcgggcata atttttatcg
    12241 tgtgttttat ggatggcttt ctgcatcagg cgtcgttcgt cacgggaaat tggtgctatg
    12301 atcggcattg ctcagtccgg ttggtgattt gttttgattt ggcgattgat cagatcgcac
    12361 aatccgggct gagttccctc aaagtgatct actattccgc gcagctattt agctgcagaa
    12421 caatagtaag cgtcaacgga gcaccgtatt gacgcttatt tattggtgag aactacgttc
    12481 catggcagga gttcgtcaac acggttggag ggccattccg gcagtacgct cagaatatgg
    12541 cgcagatacg cttccggatc gataccgttc agacggcagg tgccgatcag cccgtacagc
    12601 agtgctccac gctcgccgcc gtgatcgcta ccgaagaaca cgtaattttt ctttccgaga
    12661 cagactgcac gaagcgctct ttccgctgtg ttattgtccg cctccgccag accgtcatca
    12721 cggtaataac agagggcgtc ccactgattc agtacatagc tgaacgcttc gcccagtctg
    12781 gattttttcg acagcgtgcc attcttctcc accatccatt catgcagcga cgtcagtaac
    12841 gctttgcttc gctgctgcct gactgcaaga cgctctgact ctggtaatcc ccgtatttca
    12901 tcctcgatgg cgtacagttc actgattcgc ttcagggctt cttctgccat cgcacttttg
    12961 ctgctgatgt atacatcgtg gatttttctc cgggcatgag cacagcacgc aacttctgtc
    13021 agcgcaccac cttcgcgttc tgcactgaac agcctgtcgt aacctgtgaa cgcatccgcc
    13081 tgcaggatac cccggaaggg gcggaggtgt tgctccgggt gtttcccctg cctgttcggt
    13141 gagtacgcga accagaccgc cggaggagat gacgaacccg cattgcgatc atcccggaca
    13201 tacgtccaga tgcgccctgt tttcgtcttt ttcaggcccg gtgccagtac ctttaccggt
    13261 gtgtcgtcag tgtgaagctt gcgggtattc atcacataac gatacagggc atcattcagc
    13321 ggcgtcatta actggcagca cgcgtcaacc cagttggaga gtaatgcacg gctcagttcg
    13381 acaccctgtc gggcaaagat ttcactctga cgatacagtg gcaggtgttc gcagtatttt
    13441 cccgtaaaca cgcgggcaag taatccgggg cccgcgatac cacgctctat cgcggcggga
    13501 cggcgccggt gcttcaacga tgcagtcaca ttttgtacag gcttttttta cccgttctgt
    13561 gcggatcact ttcagagcgc tgctcaccag ttccagttgt tctgcactga cttcccccag
    13621 ataatccagc tcaccgccac actccgggca acagctttct tcagactcca gacggtgtat
    13681 ttcacgggga aggtgtgccg ggaacggacg acgatggcgc gactgccgca actggcgggg
    13741 aacctgtgga tcgtcttccc gcccactgta acgatcgctg tcctgttcgc gttgtttcag
    13801 cagggcctca gcctgttcaa cttcacgacg cagtttttca gaacaggtac cgaacagcat
    13861 ccggcgcagt ttttctatct gagcccgcag atgttctatt tcccgttcat cttcttcgat
    13921 cttttcttcg gcacgtgcca gtgcagagcg caggaaggtc ttcgtctctt caaccagact
    13981 cagttgctgg tctttctgac ggagctggct ttccagttct gcaatgcgaa tgaggtattt
    14041 ctgactcatg gccgttttta taatgcggcc aggcattttt tacaacattg tcagtgagtt
    14101 aaggcggaat gtttttggct gacgccagtc cagcttatcg aggagcattg ccagttgcga
    14161 gcgggtaatg gataccttac cgtcacgcac cgcaggccag ataaactggc cttcctccag
    14221 gcgtttggtg aacaggcaca gaccatcagc atcggcccag agtattttaa cagtgtcacc
    14281 ccgtcggcca cgaaagataa acaggtgacc tgagaagggg gtctcatcca gcacatgttg
    14341 tacctgttct cccagaccgt tgaaggattt acgcatatcg gtaacgccgg ccacgagcca
    14401 gatgcgggta cctgatggga gtgagatcat cttcccctcc cggtcagttc acggatcaac
    14461 accgtgagca gctctggtga aggatttccc agtgtcattt taccgtaatg gaatagttgg
    14521 aggcattcta cgagaaatac gtccttctct ttgccagaga cgtagccatt taaaaatcag
    14581 gttgttatca acgccatgtt cccgggccag ttgtgcaaca ttggcatttg gtttcgaagc
    14641 cagttcaacc atatgaagct taaattcatt agaaaatatc cggcgcgtcc cagagcgcca
    14701 gtctgtaggt ttatccatca atagaactcc gtctaattta gaaggagctc taatttagtc
    14761 tgcaaggatc aggagttaca gacggcattg gctttacgct tacgaacaat atcgactgcg
    14821 aatcgggaat tatctttgac aacgatgagg ataaaacaga ttcggcagta ctgtttccct
    14881 ggattgaacg tgcatgtcag gacgttcgcg agctgtgtca gtttcaacgt ctgcaaaaga
    14941 cccgtacaga ttaactccct gtccgtactc attacacaca tacacgtgcc agcccccacc
    15001 ggctggcgct ttgctttgta taaaggaaat aaataatgtc agaacctact ctcaccaacc
    15061 ccgaagtatt aaccgatcat accgatgtga tttgctccac cagtattgaa cgcatcgtca
    15121 ccggacgtaa tgcggcgctg acacagattg aagcattgct ccaccagatt aacgatattt
    15181 cgacacaaac cctcagtatc ggcggaaaaa ccgccctgga ctgggtcatg aaacaggatt
    15241 ttcgctgtgg ttgctggctt atggagaagc ctgaaacagc gatgaaagcc attacccgca
    15301 atctcgatcg cggtaaatgg cgggatttaa tgaaacgctc agggatgctg tcgattatgg
    15361 acgctcaggc ccgtaaccag tggtacaaca gcctagaaaa ggatgatatt ccggtggtca
    15421 gcgaagcgaa tattctgagc acctttaagc aactgcattt gaacaagggt gaggtgtttt
    15481 agcgtggcgt cattaacgtt ttcaaaggat taagctggaa ttttaagacg aattcgccct
    15541 gcgggtttgg agcgaaaatc attgtgacgg gactgatcaa attcgaccgg tggggattcg
    15601 ggcttaacca tggctgacag cgtgaccggt tagcagacct tgagcgaatg ctgatgatcc
    15661 ttgacgggaa gcctgttccc gacaaccgcg ctgacgttac ccgtcggctg ggagaccata
    15721 ttcatgaaaa ccggcacagc aaccactatg aggatgagat gtttgtgatt aagtattttc
    15781 agaagggaac tgcacaaatc gtattcaagc gacctgagct gattgataag ctgaacaaca
    15841 tcatcgcccg acaccaatcc gggagcactg ccagcgagat gagaggtgag tgagttgtct
    15901 gaagctattt caggtgagcc agtgcaagcc ggacggcaat ggcatactgg atggcatacc
    15961 gaaaaaacaa gcatataaaa ggccatgcta atcaacagca tggcctcgaa tagtggcgga
    16021 acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa
    16081 ctaccgctcc gcgttgtgtt ccgttgggaa cgaggcgaat attacggatt gcctcaccct
    16141 tcgtcaacgg tttttctcat cttttgaatc gtttgctgca aaaatcgccc aagtcgctat
    16201 ttttagcgcc ttttacaggt atttatgccc gccagaggca gcttccgccc ttcttctgca
    16261 ccagatcgag acgggcttca tgtgctgcaa gctcttcatc tgtcgcaaaa acaacgcgta
    16321 acttacttgc ctgacgcaca atgcgctgaa ttgttgtttc accttgttgc tgttgtgtct
    16381 ctccttccat cgcaaaagcc atcgacgttt gaccaccggt catcgccaga taaacttccg
    16441 caaggatctg ggcatcgagt aatgccccat gcagcgttcg tttactgtta tctatttcgt
    16501 agcgggcaca taacgcatcg aggctgttgc gtttaccggg aaacattttc ctcgccaccg
    16561 caaggctatc ggtgacctta cagaaggtat tggtcttcgg aatatcgcgc ttaagcaacg
    16621 aaaactcgta gtccataaag ccgatatcaa acgctgcgtt atggatcacc aactccgcgc
    16681 cgcggatata atccatgaac tcatcggcta cctcggcaaa cgtgggctta tcgagcaaaa
    16741 attcatcggc aataccatgt acgccaaagg cttccggatc caccagccga tcgggtttga
    16801 gataaacatg gaagttattg cccgtcaggc gacggttcac cacttcaacg gcaccaatct
    16861 caatgatctt gtggccttca tagtgcgcac caatctggtt cataccggtg gtttcggtat
    16921 cgagaacgat ctggcgtgta attgcagtgc tcatagcggt catttatgtc agacttgtcg
    16981 ttttacagtt cgattcaatt acaggaagtc taccagagat gcttaaacag gtagaaattt
    17041 tcaccgatgg ttcgtgtctg ggtaatccag gacctggggg ttacggcgct attttacgct
    17101 atcgcggacg cgagaaaacc tttagcgctg gctacacccg caccaccaac aaccgtatgg
    17161 agttgatggc cgctattgtc gcgctggagg cgttaaaaga acattgcgaa gtcattttga
    17221 gtaccgacag ccagtatgtc cgccagggta tcaccaagtg gatccataac tggaaaaaac
    17281 gtggctggaa aacagcagac aaaaaaccag taaaaaatgt cgatctctgg caacgtcttg
    17341 atgctgctct ggggcagcat caaatcaaat gggaatgggt taaaggccat gccggacacc
    17401 cggaaaacga acgctgtgat gaactggctc gtgctgcggc aatgaatccc acactggaag
    17461 atacaggcta tcaagtcgaa gtttaagcct gtggtttacg acattgccgg gtggcaccaa
    17521 ccgcctggcg aattcgcggt ttattcttgc tctgtttcat cggatttagc gttaaaggaa
    17581 tagtccgttt ccgggcaaca ataagttgta agcaaccaag cgcaggaata tgcgcattca
    17641 atagttttcc tccgtgtttg ttccacggaa gaacgtggaa acggctggcg tgtagcactt
    17701 caaaattcaa caaagagagc cagtccagct gccgcatcag agtgaacatc cggctgttat
    17761 agggcgatgt tttgcgcaat accggcacaa gtttgcgtaa tcccattaaa ctgatgggat
    17821 tgaagccact aatcaccagc cagccatcat caatcagtac ccgatcggct tcacgcaata
    17881 aacgatgcgg atcagtgcac cacggcaatg tatgtgccag tagacaaaca tcaacggatt
    17941 tatcggcaaa aggaagatga agtggatccg cctgcacctg gacgggcatt ccttgcgcag
    18001 aaacattcac ttgatgagaa accgcgcacg cttcgcaatt gatttctgcg cttaaattgc
    18061 caatcttaag cagatgaaaa ccatacattt tagtgaacca cgggttgagc tgacgctcca
    18121 gcgccttgcg ataaagctct ccccagggca aatcgcccca gcaatcagga gccacgacag
    18181 tttgagggac tcttgccggt ttcatcacaa ccttccgttt cacactgaga ggtaatctat
    18241 gaatcttaac agtattcccg cctttgatga caattacatc tgggttttga atgatgaagc
    18301 aggtcgctgc ctgattgtcg atcccggaga cgcagagcca gtattaaacg ccattgccgc
    18361 caataactgg caaccggagg ccatatttct cacccaccat caccacgatc acgttggcgg
    18421 cgtaaaagaa ctggtgaaaa agtttccgca aattgtggtg tatggtccac aagagacaca
    18481 agataaggga acaacacagg tagtcaaaga tagcgaaact gccttcgttt tggggcatga
    18541 atttagtgta attgccacac cgggtcacac tttaggacat atctgttact tcagtaaacc
    18601 ttatctattt tgcggcgaca cgctgttttc tggtgggtgt ggtcggttgt ttgaagggac
    18661 accatcacaa atgtatcaat cacttaaaaa gttaagtgcg ttacctgacg atacattggt
    18721 atgttgtgct catgaatata ccttatcaaa tatgaagttt gctttgagta ttcttccgca
    18781 cgatttgtcc ataaatgatt attatcgtaa agttaaggag ttacgggcaa aaaatcaaat
    18841 aacactaccc gtaattctga aaaatgagcg gcaaattaat gtttttttaa gaacggaaga
    18901 tattgattta attaatgtaa ttaatgaaga aacattattg caacaacctg aagaacgttt
    18961 tgcatggtta aggtcaaaga aagataggtt ctgataaaac tttcttgtca tcggctccgt
    19021 tcgccgttat gatcggtcgt cttttaagca actattgaca cacacatgaa ggcaaaagcg
    19081 atattactcg cctctgtcct gctcgtgggt tgccagagta ccggcaacgt tcaacagcac
    19141 gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa agtttacaag tcaggcacga
    19201 tggatggacg atgggacgtc tatcgcgcca gatggtgact tgtgggcttt cattggcgac
    19261 gagctaaaga tgggaattcc ggaaaacgac cggattcgcg aacagaaaca gaaatattta
    19321 cgcaataaga gctatctcca cgatgtaact ttacgggcag agccgtatat gtactggata
    19381 gccgggcaag ttaaaaaacg taacatgcct atggaactgg tactactacc catagtggag
    19441 agcgcttttg atcctcacgc aacgtctggc gccaatgccg cgggcatctg gcagatcatt
    19501 ccgagcacgg ggcgcaatta tggtttgaaa cagacacgca attatgacgc gcgtcgcgat
    19561 gttgttgctt caacaactgc cgcgctgaac atgatgcagc gtctgaacaa gatgtttgac
    19621 ggcgactggc ttctgaccgt agcggcttat aacagcggcg aaggtcgggt catgaaggca
    19681 attaaaacga acaaagcgcg tgggaaatcc acggacttct ggtcgttacc gttgccgcag
    19741 gaaacgaagc agtacgtgcc taaaatgctg gcattgagtg atattctcaa aaacagcaag
    19801 cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg ctctggcgcg tgtgcacctg
    19861 agcagcccgg ttgaaatggc gaaggttgca gatatggcgg ggatttccgt cagcaagctg
    19921 aagacattca acgcaggcgt gaaaggctcc acgctgggcg caagtggtcc gcagtacgtg
    19981 atggtgccaa agaagcatgc agatcaactg cgtgaatctc tggcttcagg cgaaattgct
    20041 gctgtacagt cgacgctggt tgccgacaat acgccgctta acagccgtgt ttacaccgta
    20101 cgctctggcg acacgctttc aagtatcgct tcacgtctcg gcgtaagcac caaagatttg
    20161 cagcagtgga acaaactgcg cggatctaag ctgaagccag gccaaagttt gacgattggt
    20221 gcaggcagta gcgcacagcg actggcaaac aacagcgata gcattacgta tcgtgtgcgc
    20281 aaaggcgatt cgctttcaag cattgctaaa cgccacggcg tgaacatcaa agatgtaatg
    20341 cgctggaaca gcgacactgc gaatctgcaa ccaggcgata agctgacgtt gtttgtgaaa
    20401 aacaacaaca tgccagattc ctgacaaacc agataataaa aaaggcaccg attcccccgg
    20461 tgcctttttt atttatgccg ctttatgtgc ttctagcatg atgatatcac tggtgaaaga
    20521 gccatcattc tgcaaggcaa aatacgtttt cacctctgtc gatgcgctct gttggtaaat
    20581 gcgaatagcg tctactaacg cttctggcgt acgcattctc gctacccatg aagaaaattc
    20641 cagcggtaac ttatctgtaa ttaaattatc aactatcaga ttggcttcac tgattaacgt
    20701 caaccactca ccgctggcgt agttccgtgt gtgagatata tcgcgtaatg cttctaccgt
    20761 ctgtaaccag atgtcgcgca ctgggtggcc cggagacatt acgtccatca caatcagctt
    20821 accgccaagt ttcaatatcc ggttcacttc acgcagtgct gcaccaacat catgccagtg
    20881 atgggcagaa taacggctga taacaatatc aaatgcgtta tcggcaaatg gcaggctttc
    20941 gacataaccc tggcgggtgg tgatattttt cagttgccgg gcttcggcag cttgtgccac
    21001 gacatccagc atatgggcag ataagtcata cgccaccacc gtgctcacgt tttgcgcagc
    21061 gacaaagctg gcatgccctg ctccgcagcc catatcaagt acacttgcat caggataatc
    21121 agccagacgc accgccaggc gctgtaaatc tcgtccggat gcatgtacgg tactggtcag
    21181 atattcgctg gcttgagagc taaactgctt ttctacgtgg tcatggtggg attgtgttgt
    21241 cattgcgctg tccttttgtt atttgagtat aaaagggcag cgccaaacgt tggtctgccc
    21301 tgtggcagac ctgacatacc ttatttatca ggcttgccgg gactgaattc aacgagtagc
    21361 ggattgtgat cggaagcgcg cgtaaccagt acagaagctt cgctgacgtt cagaccacgg
    21421 tagaaaacaa aatcgagcgg gcggccaaac gcccgacggc gctgatcatc agtaaaacgc
    21481 acctggcgca gcgacatttc ccgcgcaaag cgatataacg cgttcatcct tctacggctc
    21541 caggcattga aatctcccgc cataatgacc gggccgctgt ggtgagctat ctgatcgcca
    21601 ataggaagta actgcttact atagacatcc acgcccagac tgaaattgac ggcgtgtata
    21661 ttaaccacca tcaacaggcg agtgtcaggt aagggataga ccgtcaccag ggccgacttc
    21721 gccagacgca aaatgggttc tcgttcacgt aacgggcagc aatacactgg atgcgccgcc
    21781 gaaagggtca ttacgccaga aggatgttgt ggcagtacga aagcgggtac ctgatcggcg
    21841 gcaagatagt tagcggtcgc aaactgtact aactctggcg ttgtctgcgc ttcctgcaat
    21901 aacaccagat gtgcatcttt gccgtagttc tttaataccg acaaccattc agcgcgttgc
    21961 tgtttgtata tgttccacac caggatccga atacgctctt cggtacttaa cggttctcca
    22021 ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg gtaagatcct ttccgcaggt
    22081 tgtccggcaa catagcgcat ggcataggtg ttttttcgca cttttgactt tgaaacctct
    22141 atcacgtcaa cccttccgca ctcgggaggg ttctactgtt atagggattt tagctcactc
    22201 tttcaacgag caattgtata ttgttatgta agcaagtgct tacaccggtc aggattttgc
    22261 tcattattga gaacattaaa tcggatggca tatctcgcat cctgatggtt taatgacaaa
    22321 taaattcaaa atatctcttc tattttgcaa caggagcaaa aatgaaagcc acgtcggaag
    22381 aactcgccat ttttgtttca gtcgtcgaaa gcggcagctt tagccgggca gcggaacaat
    22441 tagggcaagc aaactcagcg gtaagccggg cggtgaaaaa gctggagatg aaacttggcg
    22501 ttagcctgct taatcggacc acgcggcaac ttagcctgac ggaagaaggc gagcgttatt
    22561 ttcgtcgcgt acagtcaatt ttgcaggaga tggcagcggc agaatcagaa attatggaga
    22621 cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc aactccagtg gtgctgcact
    22681 ttctgatgcc gttgattaag ccttttcgtg aacgctatcc ggaagtcact ttatcgctag
    22741 tctcctccga aacgattatt aatttgatcg aaagaaaagt ggatgtcgcg atacgcgccg
    22801 gtacgttaac ggattccagc ttacgtgcca ggccgctatt taacagttat cgaaaaatta
    22861 tcgcctcccc cgattatatt tcccgctacg ggaagccaga aacgctcgac gatttaaagc
    22921 aacatgtttg cctgggattc actgaacccg cttccctcaa tacctggccg atagcctgta
    22981 gcgatggaca attacatgag gtgaagtacg gtttgtcatc caatagtggg gaaacactga
    23041 aacagctttg cctgagtggc aacgggattg cgtgtttgtc cgactacatg atcgacagag
    23101 aaatcgctcg cggagaattg gtggagttaa tggcagataa agtgttgcca gtggaaatgc
    23161 cattcagtgc agtctattac agcgaccgtg cggtaagtac gcgcatccgg gcttttatcg
    23221 atttccttag cgagcatgta aaaacagctc acgaaggagc tgtcagagag gcttaatccc
    23281 attcaggagc cagaccttcc gggctaacca gacggacgtt gcaatccagt gcggcgatcg
    23341 cttttttatc ttcggcatca agctgtaaat tttgtgcctt aagattactt tccaggtttt
    23401 tacgtttagt agaagaagga attacggagt aatcttcccc catagcccac gccagaatca
    23461 cttgtgccgg agtcgcattg tgtttagctg cgatacgagc aataacctca tctttcaggg
    23521 acttaccata cgccagcgtc atataggaag taatatggat gccgtgctgt ttagcccggg
    23581 cgaccacttt acggttttgc agataaggag agagttcaat ctggttagta gcgatgtttt
    23641 cagcgccaac agcagcaatc gccttttcca tcaatgggat cgtgaagttg gaaataccga
    23701 tctcacgcgt cagcccttgt tttttggctt ccagcagcgc ctgcataaac tcttcagcag
    23761 agacttcatc gtttggtgac ggccagtgga ttagcgtcag atcaacataa tcggtacgca
    23821 atttttgcag gctctctttc agactcggga tcaatttgtc tttgctgaga ttttcaatcc
    23881 agattttagt ggtgatgtag agttcatgac gtggcacgcc actttctgca atcgcctgac
    23941 ctactgcggc ttcgttatca tagatttgtg cggtatcaat tgcgcgataa ccaagttcta
    24001 gcgccgtttt cacagatgaa ataacaacgt cgtctttcag acggaaagta cctaaaccaa
    24061 atgcagggat agccataata tgcctctttt aatgctatgt tcgttaactg aggtgattat
    24121 ggactttaga ctgttgcgga aaaagggcgt aaaacgcaga ggatttttgc gattttggca
    24181 ataatagaaa tacagacgaa aaaaaacctc gagcgttaac tcgaggcttc ttaataagtg
    24241 gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccga
    24301 ctgaactacc gctccaccga attcttttac aaccaccggt tttatgaccg gcttactgct
    24361 aaatttgatg cctggcagtt ccctactctc gcatggggag accccacact accatcggcg
    24421 ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctaaggccg
    24481 ccaggcaaat tctgtttcat taacccgctt gtcgccggtt aatctaatct gtatcaggct
    24541 gaaaatcttt ctctcatccg ccaaaaca
//
LOCUS       DAZFRN010000070        23697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFRN010000070 DAZFRN010000000
VERSION     DAZFRN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.070"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            167..2839
                     /gene="rcsD"
                     /locus_tag="ACHKUB_002672"
     CDS             167..2839
                     /gene="rcsD"
                     /locus_tag="ACHKUB_002672"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO6655665.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2856..3506
                     /gene="rcsB"
                     /locus_tag="ACHKUB_002673"
     CDS             2856..3506
                     /gene="rcsB"
                     /locus_tag="ACHKUB_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO6655666.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3706..6555)
                     /gene="rcsC"
                     /locus_tag="ACHKUB_002674"
     CDS             complement(3706..6555)
                     /gene="rcsC"
                     /locus_tag="ACHKUB_002674"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO6655667.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(6830..7606)
                     /gene="yfaP"
                     /locus_tag="ACHKUB_002675"
     CDS             complement(6830..7606)
                     /gene="yfaP"
                     /locus_tag="ACHKUB_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO6655668.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(7611..9260)
                     /gene="yfaQ"
                     /locus_tag="ACHKUB_002676"
     CDS             complement(7611..9260)
                     /gene="yfaQ"
                     /locus_tag="ACHKUB_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO6655669.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKASTLPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWATGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(9261..13775)
                     /locus_tag="ACHKUB_002677"
     CDS             complement(9261..13775)
                     /locus_tag="ACHKUB_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO6655670.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(13799..14422)
                     /gene="yfaT"
                     /locus_tag="ACHKUB_002678"
     CDS             complement(13799..14422)
                     /gene="yfaT"
                     /locus_tag="ACHKUB_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO6655671.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(14419..16107)
                     /gene="yfaA"
                     /locus_tag="ACHKUB_002679"
     CDS             complement(14419..16107)
                     /gene="yfaA"
                     /locus_tag="ACHKUB_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO6655672.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQAQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(16256..18883)
                     /gene="gyrA"
                     /locus_tag="ACHKUB_002680"
     CDS             complement(16256..18883)
                     /gene="gyrA"
                     /locus_tag="ACHKUB_002680"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO6655673.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            19030..19752
                     /gene="ubiG"
                     /locus_tag="ACHKUB_002681"
     CDS             19030..19752
                     /gene="ubiG"
                     /locus_tag="ACHKUB_002681"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO6655674.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(19880..23614)
                     /gene="yfaL"
                     /locus_tag="ACHKUB_002682"
     CDS             complement(19880..23614)
                     /gene="yfaL"
                     /locus_tag="ACHKUB_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO6655675.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSVSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQMRLGLHSEWRTAVHVIPTLDLNYYHNPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
BASE COUNT         5678 a         6480 c         5806 g         5733 t
ORIGIN      
        1 tatttgtaca gcaaaatggc gcttgtacat ctatttcccc caatgcagga tgataaatat
       61 cacgggagaa tagagaatca tcaatcaggt aagagtctgg aatttcacac tgtacccttt
      121 atactgccct atcacttcgc gaagttttaa caggtcataa acacgaatgc gtcagaaaga
      181 gacaacggcc acgacccgct tttcactcct accggggagc attacccgct tctttttact
      241 gttgatcatt gtgttactgg tgacgatggg ggtaatggta caaagcgccg ttaacgcctg
      301 gctgaaagat aaaagttacc agattgtcga cattacccac gctatccaaa agcgcgtcga
      361 tacctggcgt tacgtgacct ggcagatcta cgacaacatt gccgcgacga cctccccctc
      421 ctccggcgaa ggtttacaag agacgcgcct gaaacaggat gtctactatc tggaaaaacc
      481 gcgccgcaaa acggaagcgt taatctttgg ctctcacgac aactcaacgc ttgagatgac
      541 tcaacggatg tccacctatc tggacacatt gtggggcgca gaaaatgtac cgtggtcgat
      601 gtattacctg aatggtcagg ataacagtct ggtgctgatc tcaaccctgc ccctcaaaga
      661 tctcacctcc ggatttaaag aatcgaccgt cagtgacatt gttgattcac gtcgtgcaga
      721 aatgttgcaa caggccaacg ccctcgatga acgcgaaagt ttttctaaca tgcgccgcct
      781 ggcctggcag aacggtcatt actttacctt gcgtaccacg ttcaaccagc cgggacatct
      841 ggcaacggtc gtggcttttg atctgccgat taatgatttg atcccaccgg gtatgccgct
      901 ggacagtttc cgccttgagc cagacgcgac ggcaacggga gacaatgata atgagaaaga
      961 agggacggat agcgtcagta tccactttaa cagtacgaag attgaaatct cctcggcact
     1021 caactctacc gatatgcgcc tggtctggca ggttccttat ggcaccttat tgctggatac
     1081 gttgcaaaac attctgctgc cactgctgct gaacatcggt ttgctggcgc tggcgttatt
     1141 tggctatacc acattccgcc atttctccag ccgcagtaca gaaagtctac ccagcacggc
     1201 ggtcaataac gaattgcgca ttttacgggc aattaatgaa gagatagtct cactgctgcc
     1261 gctcggcctg ctggttcacg atcaggaatc gaaccgcact gtcataagta acaaaattgc
     1321 cgatcatttg ctgccgcatt tgaatctgca aaacatcacc accatggcgg aacagcatca
     1381 ggggattatt caggcgacga tcaataacga gctgtatgag atccgcatgt tccgcagcca
     1441 ggtcgcgccg cgcacacaaa ttttcattat tcgcgatcag gatcgcgaag tgctggtaaa
     1501 caagaaactc aagcaggcgc agcgtctgta tgagaaaaac cagcaggggc ggatgacctt
     1561 tatgaaaaac attggcgatg cgctgaaaga acccgcacag tccctggcgg agagcgcggc
     1621 taaactcaac gccccggaaa gcaaacaact ggcgaatcag gcagatgtgc tggtgcggct
     1681 ggttgatgaa atacagttag cgaacatgct tgcggatgat agctggaaaa gtgagacggt
     1741 gctgttctcc gtgcaggatt taattgatga agttgtgcct tcagtgttgc ctgccatcaa
     1801 gcgtaaaggt ctgcaactgc tgattaacaa tcatctgaaa gcacacgata tgcgccgcgg
     1861 cgatcgcgat gccttacgac gtattttgct gctactgatg caatatgccg tgacctcaac
     1921 gcaattggga aaaatcaccc ttgaggttga tcaggatgag tcctccgaag accgcctgac
     1981 gttccgcatt ctggacacgg gagaaggcgt aagtattcat gaaatggata atttgcactt
     2041 cccgtttatc aaccagaccc aaaacgatcg ctatggcaag gcggacccgc tggcattctg
     2101 gctgagcgat caactggcac gtaaactggg cggtcattta aacatcaaaa cgcgggatgg
     2161 gcttggtaca cgctactctg tgcatatcaa aatgctcgca gctgacccgg aagttgaaga
     2221 ggaagaagag cgtttactgg atgatgtctg cgtaatggtg gatgttactt cggcagaaat
     2281 tcggaatatt gtcactcgcc agttagaaaa ttggggtgca acctgtatca cacccgatga
     2341 aagattaatt agtcaagatt atgatatctt tttaacggat aatccgtcta atcttactgc
     2401 ctctggcttg cttttaagcg atgatgagtc tggcgtacgg gaaattgggc ctggtcaatt
     2461 gtgcgtcaac ttcaatatga gcaacgctat gcaggaagcg gtcttacaat taattgaagt
     2521 gcaactggcg caggaagagg tgacagaatc gcctctgggc ggagatgaaa atgcgcaact
     2581 ccatgccagc ggctattatg cgctctttgt agacacagta ccggatgatg ttaagaggct
     2641 gtatactgaa gcagcaacca gtgactttgc tgcgttagcc caaacggctc atcgtcttaa
     2701 aggcgtattt gccatgctaa atctggtacc cggcaagcag ttatgtgaaa cgctggaaca
     2761 tctgattcgt gagaaggatg ttccaggaat agaaaaatac atcagcgaca ttgacagtta
     2821 tgtcaagagc ttgctgtagc aaggtagcct attacatgaa caatatgaac gtaattattg
     2881 ccgatgacca tccgatagtc ttgttcggta ttcgcaaatc acttgagcaa attgagtggg
     2941 tgaatgttgt cggcgaattt gaagactcta cagcactgat caacaacctg ccgaaactgg
     3001 atgcgcatgt gttgattacc gatctctcca tgcctggcga taagtacggc gatggcatta
     3061 ccttaatcaa gtacatcaag cgccatttcc caagcctgtc gatcattgtt ctgaccatga
     3121 acaacaaccc ggcgattctt agtgcggtat tggatctgga tatcgaaggg atcgtgctga
     3181 aacaaggtgc accgaccgat ctgccgaaag ctctcgccgc gctgcagaaa gggaagaaat
     3241 ttaccccgga aagcgtttct cgcctgttgg aaaaaatcag tgctggtggt tacggtgaca
     3301 agcgtctctc gccaaaagag agtgaagttc tgcgcctgtt tgcggaaggc ttcctggtga
     3361 ccgagatcgc taaaaagctg aaccgcagta ttaaaaccat cagtagccag aagaaatctg
     3421 cgatgatgaa gctgggtgtc gagaacgata tcgccctgct gaattatctc tcttctgtga
     3481 ccttaagtcc ggcagataaa gactaatcat ctgtaggcca gataagacgc gttagtgtct
     3541 tatctggcat ttgcaccgat tgccggatgc agcgtaaacg ccttatccgg cctacgattc
     3601 ccattatttc aacagattac attaaagtag gccagataag acgcgtcagc gtcgcatctg
     3661 gcatttgtac tgaatgccag atgcggcata aacaccttat ccgtcctacg attcccgcga
     3721 tttcctgacc ctctcggcat ataccgtcag cgtctgtttt atcacatcca gcgttaccgg
     3781 cttcgacagg cagctgtcca taccggactc cagacaccgc tgcttctctt cagccaacgc
     3841 attagcagtt actccgatta ccggcaacgt cagtcccaac tgacgaatgc gttgcgtcaa
     3901 gcggtaacca tccatatttg gcatgttgac gtcgctaagc acgatatcaa tatgattctt
     3961 gctaagtaca ttaagcgcat cgacaccatc attcgcggtt ttacattgat agcccaacga
     4021 tcccaactga tctgccagca gacgtcggtt aatcggatga tcatccacga ccagaatcat
     4081 catatcgtca ttatcgctga ccgctttgtc cgtcgacggc agagcgttag caggatcgtc
     4141 gctctccatc tcgatcaaat aaatacgcgc caacaatgcc ggtagctcat gcggggcagc
     4201 cacactgtgt acccactccc ctggcgcttt ctccagcgga ataccaatat ggcgacgaca
     4261 gaaggtcact accgctctac cctgccattt tttactcact acctcgtcag taattaacac
     4321 atcttcggga gtcggttcct gcccttcgta tgttgtaacg acgatgccgc tgcgctgcaa
     4381 actggtttcc aggaactgac agagcgacgc attgcggacc gccagccagc agcgtttacc
     4441 actcaaccct tccacgcctt ttttctgcgg gtactgagcg ccgtacaacg gaatacgcac
     4501 ggtaaactgg ctgcccattc ccggttctga atctaccgag atatcgccgt ccatcatgct
     4561 gatcagtttt tcacaaatcg ccagacccag accggtcccc tggaagtttc gctgtacgcc
     4621 cgttccgacc tggaagaagg gatcaaacaa gcgcaccact tctttcgccg gaatccccac
     4681 gccggtatcg cgaacacgga tagagagata atcgccatca gcgcggacat gcaaaactat
     4741 acagccggta tcggtgaatt ttatggcgtt actcaacagg ttggagatga cctgctgtaa
     4801 acgcatcggg tcgccattta aggccactgg cacatccggt tcaataaagc agtacaagcc
     4861 taactgcttg cgtaccacca gcggtaaata gttggcggtg atgtggttca tcacttcacg
     4921 cggtgaaaac tcacgcggtt cgatcttcaa ctgttccgat tcaatcttcg agaaatcgag
     4981 aatatcgctg ataattttca acaacaggct ggaagagttg ttcattgccg tcaccagccg
     5041 atcgacgcct ttcggtaact ctttggtttg caacagatcc aggttaccga taatgccata
     5101 cagcggcgtt cgcagctcat gactgacggt ggcaaggaac atcgattttg actggctcgc
     5161 ctgttccgct gcttgtgcca tctcctgcaa cgactcttcc atcttcacgc gcgaagaaac
     5221 atccaccagc acacaaatgg ccacgttttc attacgatag cgcgaatgga cgaagctgat
     5281 ttgcagattg gtattgttgc tggtcaggac atcaacaaaa ttgacctgct gcccacagat
     5341 aatttgcgtc agtcgttggc ggtcctcatg cgtaagcata ttgagatagg tatgcgccag
     5401 ttcgttactt aaaatattga cgccatcagc ggtacgcaaa atgcagatac ccactggcgc
     5461 ggaggcgaca atcttgcgat tgaactgctc atgttcttcc agtcgcaggg cgtcgctttc
     5521 cgccggaatg aaaatacgtc gctcgtacat ccgcgcgaga gtaaacaatg cagctccggc
     5581 aagcacattc agcaaaattg cgttaaggat caacatgcga atgcgttcca gcaccttatc
     5641 aaccggcacc gaatacacga tgcttagcga tgagggtggc agatttttct tcagcaccag
     5701 ctcccggaac ccttccgtat agccaaacca ggagcgttcc tgcatccagc gaggatcgcc
     5761 cttaatttta ctttctggtc cggtaagcga aatcagggta tgaccatttt catcaagaat
     5821 ggtaaccccc atcggcaacg tacccggtaa gaaaaagttc tccatccgga tggtctgctc
     5881 gacacccaaa agcgcctgca accggttcgc cagataaact ggcgtcaacg cgtaaaaata
     5941 cccgacgcca gggcgcggac cttcgctgat ccagtagagg ttactgccgc tatcatcttg
     6001 tggtgcattt cgatatttat tgatgcgttc atgcaaagct ttcaacgcgg tatcgcgttc
     6061 cactggcata tcacgcagac cgaaattggc catgcagagg ttatcactgc cgattaaaaa
     6121 tacccggttg agatcgtaag ccgcagaaaa attatcgcgc cagtagcgca taaaccacgc
     6181 caatgactcc agagaacctc gccaggtgtt actcattgcg gaacaatcgg agtcggcaaa
     6241 cagcggttca aacgcaggca catccgcctg cgtttctcgt ccacgcgggg aaagcacacc
     6301 gttttctgcc gataagcgat tttcggcgat gtacttcagc tctttcatca catcagaggt
     6361 gcgttgaata aagcgctgag cctgatcgga actcagatta aattcctgac gaatttccga
     6421 ttctcgctga tgtaacgcat taacgatgta aaaaacggat gaaaaagcaa tcaacagcca
     6481 gagcactaac gccaatgctc tgaacatgta gcgcgaggct ttcagggttg tacgaaaaga
     6541 agcaaggtat ttcaaagggg cgaagctccg cctcaggtga ccgatggagt gtggttaagg
     6601 tagcggtaaa agcgtgttac cgcaatgttc tgtcaccatt gcaataatgc gtatcgctta
     6661 gaaaccattg ccttaaccag gaaagccgga gtagatggac agttctcagt acgaaaaata
     6721 agaaatgacc aatctgccga cgcttgttaa tggttatatc ctggcgtcgg cagatcgccc
     6781 agcagtctta tctgaaaaaa gagagagcca ggttgtgtga agggatggat caccagtcaa
     6841 aacttttcac cagcgtcagc tcgccagcat tacgcatcgg tacaataaat gtttcctgtt
     6901 tctcattgac cgatccttca tcggtgatca gcgtcagttg ggcggtcgtc aattccgttt
     6961 cgctgcgccc accatagtag ttgatataca cctgatagcg cccgtgaact ggtgctggca
     7021 tggcgaaaat ctcgggtccg taccccgtcg tgacatccat atccagtgca ccactgtttt
     7081 tcagcacggt gttaccgtac caggcgtgtt cgccatcggg cgtgacaaca tggagatcga
     7141 gatcggtatt gtccgtatcc cacgagagaa ccagccgtaa acgtgcacga atcgttcctg
     7201 tacccggcgt tgagtaaaac tgcatttttt gtcggctttg cccatccggg ctgatgacct
     7261 gcacgctgtt gctgccttca gtgaaaatat aagggcgtgc aaatgaacca tcggattcaa
     7321 ctctctgcgg catactggca ccgttgacca ccagccggcc ctgctgaact ttacccgccg
     7381 cagcataatt ctttattttg ccgcggatct gcgctgagat attttgatca tctgccatgt
     7441 tgacggacga cgccgggtaa ttgatcgatt gtgaaaaact ggcatcttcg ccctctgccg
     7501 gatgccagcc agagagcggt gcgtcaatct cgacttcctg cacaccttca ctgtgagcga
     7561 caggcgaaag tgccaccagt aacaacggaa gaaaaatctt tcgcatagca ttagtccatt
     7621 aataattgtc gggtcagcgt ttcgatatag ttctcatcaa gcccggtggg ataaccatca
     7681 aaggccaggt gcagatactc atgggttaaa tccagccgat cctgaagcgt aaagaagtta
     7741 cgaataaaca gccgtttctg ctgccgatcg gtatagggga aacctgagac taaacgacag
     7801 accgcaaaca cgtctggttc gttgtacccc gtctcgcctt gtaatatacg ccgccactgc
     7861 ggcatttttt tcgccagcca ggctttcgct ttgggtaata actggcaggt tgagcgtgga
     7921 gctccccagc ggctaaggct gttgtcggga taagcaaacg cgagtatctg gtcgtaacgc
     7981 tccccctgcc ccgcctgggc cgtggcttgc cgccaggaaa gtgtcccttc ggtagcgcga
     8041 ctgccatgat aatgaaccgg atcgcctgca tagatgagat cctgcgtcca ggcggtcatc
     8101 gttcgcgccc ccgtggtcgc tggcgaagca gaaacacgct gcgtggcgct gctgtcgggg
     8161 atggtcaggc aatcgccttc ccgattggcg ttttgctgta aaaaagtgcg aatcgccacg
     8221 gtcatggctt tcgctgcctc agggggcgta cttttcgcct cgcgatccag aactctggca
     8281 acgtactctt cacgatcgag atgcgactgc aatttcagtt tgcccttttc gctcagtaag
     8341 gtcgtctcac cgtggctgac aaacgtaatg tggttaccat tggtaaacgt cacacgatag
     8401 cgcccattaa gtacgccggg ttttactgcc gtcgtactct tttccgctgt gattttcttc
     8461 agcggatagc gggcaaacag ctccacctct acacattgcc cactggcaac ctgcgtcggc
     8521 actggcaaaa cccgattaag caccgtcgca taacgggtta gtaccgtttt gctggtgcca
     8581 ctgccagtag cccacagcgg tgtaccatcg gttaaccagc cggcaaaacc accctgacgg
     8641 atttcctgtt tatcatcggc aaaccagctc caggttttca cccgtacccg actaccgagc
     8701 atggatgcga ctccgatttt cgcctcgtcc agcaccacat ccagtaagac ttcctgagct
     8761 ttgttttgcg caggtaacgt cgctaatgaa tcaagcagcg atttaaccgt taccgaggtt
     8821 tctggcttca gcgtagtaag agacgctaac cacgcaggtg cctgacgctg ttgccagtat
     8881 tgcccccaca catctgcgcc aatatgcaac cgctgcggcg caaaatagag cccacaggaa
     8941 cgtaccagcg cggtatcgcg cgttatgctc tccccagcct gacaacaata aacctcttcc
     9001 ggggagtttc cgcgacactg gtagggctgc tccggttgat gggtatcctc cagccaggcg
     9061 taaacataca gtttccacaa actccctaac ggcgtggtga gcgagtcagg taatgtcgag
     9121 gctttggtca cgcctgatga cgacagctga tacagttgat cgtgctgcgc accgcgcagc
     9181 accagttgta acggcgtctc ctccgcaagc gttggcagag taaccaacgc caatagccag
     9241 acgattctgc gccagttcac ttatttcacc tgcatcccgg tccattcact ccctgccgca
     9301 acactttgct gcgcaggtgc ataggaacgc acataacgag caggaggcag aacgaattgc
     9361 cctttttgcg agaagcgcag caaatggcgg aaagtgaccg ttcccgtcag ttctttcacc
     9421 ggcaccatat aggccagttc gcccatttca ttacgcgctt tttccagcaa ttgcccctgc
     9481 tgtttcgcgg cgttgggttt attgactgaa atgccccatg ttgtgcgctc aacgtcggct
     9541 cccggcggga gcggtacttc cacctgacca tagcgcagaa ctgcatcctg ctcgctggta
     9601 agcgtaattt catcgagata cagcgcatcg ctgtcaatct cattgctggt caccggttgc
     9661 agaataaagc tcatctcttc ttcaccaggg ataagccgat acaactggcg ttcaacggtc
     9721 accggaatgt tactttgttg agccattttt gccggctcac gccagcggac ctgcacattt
     9781 tgcggcgata attcgtcacc aaaagagaga atgtccggca cgccctgacc aacccaacgc
     9841 cagtcttcgc cccctccagt taacttatgt ttagcccatg cgcccgcagg cgcaggcaaa
     9901 acaactggag gcattgtcgc catatatttc gccagccagt tcatggccag cgcgcgctca
     9961 atagtggatt gctcagcggt caaaccactt aaaatagcgg ccgtctgggt agcgtcacca
    10021 ccgctattga gcagcacaac cgtgtgcgcc agcggctgat tgctgtttgc cgctttatcc
    10081 cacgcatatt gctgaacctg tggcatcacc gtggacttca actgcgcttt tttcagcaac
    10141 gtcatggtta agacgtttgc caccgcatct gccagtggag attccggtgt atcgaggatc
    10201 aggctgtcat tgatatcgcg gatgtcttcc tcagagaaat cttcatcttt agttccgcgc
    10261 cgggcgatag cttcatccaa ccctttcaac aacgttttgc acggcagatt catctcctgt
    10321 gcccacgcca gcaccagcgc ccgatgcaat aacggcatgt tatccgcctg ctcggcataa
    10381 ctgtcgagca tatgctgcca gtattccggt tgttgcgtta cgccgagcgc ctggctggcc
    10441 tgccagtcgg cgtaccatgc ccatgccgta aggaaggcgt caccattgcc atcttcaccc
    10501 caccaggtaa agcgcgctcc gggccccgcc agttgcatca gccgcagacg gttatcctga
    10561 atcatctgac gaatgtcgtt agcggcggca ctttgatgat cggcaagcga acgccaggcg
    10621 agactgagcg ggatcagacg gctaccggtg ttgatcacgc caccccatgg tccatcgact
    10681 aacgcatcaa gattgttgcg gaaaatctcc tgcggcgttt cactactttg tagccggata
    10741 ttactcgcct gctcgggcaa catcagcgcg ttctcgccac cgccgagcat gacatttttc
    10801 tgctgttcaa cgggccagct gttgtccaca aaagacagtt ttgtgctaat gctgtcctgc
    10861 acttgcccat tttgttgcag ttctgcactt aacaagccag attgctgaat gttctgcgtc
    10921 agcgaaatat aattcgcccc tttgtgcagc gtcagcgtct ggcgcatctc agcgcctgca
    10981 aatttagtca ccagcgccac cggttcgtta tcctgctgac tgaagataaa cagtcctgcc
    11041 gccggtttgt cgcccacgcg atacaccgtt ggcatactcc acttcatgta gagatttttt
    11101 tccgaacgca gataagcacg cccctgcccg accagcccgt cgccgttcat cccacgcgcg
    11161 gtgatacgcc agcgggttaa cgaatcaggc atcaggaacg tgaaatacgc tttgccttgt
    11221 ttatcggttg tgagtgacgg catccatgcc gcggtatcca cctcttcacg ccgtggacgt
    11281 tcaagcattt ttactcgccg ctcgctgcgg ttagttgcgc caggcgcaac cggctcgctg
    11341 gagagcgcct ggtcgtagct gataaacgac agactggagc tggtacgcac attgttacgc
    11401 cccagcggat agaaaaattt gccgatattc ggcgcgattt ctggttgcag cgcgtagatc
    11461 atttcatcga ccacgcctac cgttagctgc gcagaaacag gtttaccttt cagcgacgag
    11521 gttaattcga cattgaccag ttcaccaggc tggtaatggg ttttgtccgt tttcacccgg
    11581 atatcaagct gaggaacggc aactttgatc ccggcgttct gaaaactgta ctgaccatta
    11641 cgggtataca gcaccgaaaa agtgatgtta ggcgcaaagg aattgctcac tggaacccgg
    11701 gcttcatact gggtatcgtt taaacgttgt aacgtcagcc agtttgccgg atgcgaaagc
    11761 agcgactgct gttccacgcg atcgcgttcc agcgtcaata atgcttcatc aattggctcc
    11821 ggaaaggtaa tcagcatctt cgcagtttcg cctggctggt acagcgtttt atccgccacg
    11881 atatctaccg taccagtatg cgccgtgctg cccttaccgc tgacggcatg acttaaccca
    11941 gcgagaatta agccgtcttt atcgcgtaat gtcagattgt agttgccagg tttagcgaaa
    12001 ttgacggtaa aggatttgcc gcctgacggt agctctccgc tatggctcgt gcggtcttcg
    12061 agacgcaacc attcatacgt aacaggaacc tgttttgaag attccagcgc ggcataacgg
    12121 aacacaaccg actcgccgct attactgtat tgtgcggcgg tacttaaaga gtaatgcgcc
    12181 aggccgcgtt caatgaggat ctctttggtg gtggtgacgc gatacgccgc gccgtcactg
    12241 gcggagacgg ttaacaaata gcggctcggt ttatcggcgg cggggagatt taacgccaca
    12301 tgaccgctgg cgtcggacac cgtttcgctg ccttccagcg acacgggaaa acgtccggca
    12361 taacgcaaat cgttaccgac cattgataat tgctgagcgc gcaaacttaa ctgcacgcgg
    12421 gcatctttta ccggctcacc atccgggtaa agcagttgca gtttgccgct gaccgcttcg
    12481 ccagttttga actctttttt gtcgagagct aaaccaatct cgaaatgtgg cttgatgtag
    12541 tttgccacgc gaaaactgct gctatagacc tgattgcggt aagcaagacg taactcataa
    12601 cctccggcta cggcattttc tggcaggcgg aaacttccct gcccgccatt gcgcgcatcc
    12661 agcgtgacat tgacggtttg caacagactg ccgttagcgt ccagcaccga aagcttcgcc
    12721 ggggcgctga cgatggggga tgaatgcaac ggatcgtgga actcgcggcc catcacttta
    12781 acatcgacac gatcgcctgc gcgatatagc gggcgatcgg taaaaatata caagcgggtg
    12841 ttgtagattt cgctttcgta gaagaagttc tcggagacaa aaacaccgcc ttcagcatcc
    12901 ttacccagaa tgtatgaacg ttctggcgat atatgttgta actgcaaggt accgctgtca
    12961 tcggtcacac cgcgggtcat cacgccaaga ccgtcagtcc acaagatctc tgagccgggc
    13021 ttcgcttcac cctgtttttt acccgcggtc cacaccagaa gctcgttgcc tgacactttg
    13081 ctaagcgcca cggtatcgga aacaaacacc accgtcgtcg cccgataccc accaaccatc
    13141 gcctcgacga ggtacagtcc cggctcttgt tggccgagag gaatataaat gttacccggc
    13201 tgcggcgaga tgaaattgct ggatgcgcct tccagtttta ccccttgctg cggctcgacc
    13261 ggtttagcct gccatagtgg ataacggaac tgttccacca agggatattt tttcagcggg
    13321 gaaaactggt tgttctgtac ataacgggaa ggtttaataa tggcattgcc gagatgtaat
    13381 tcgggtaatg cctgagtcac attctgccgt gactgagaag agaaagtacg ctgcattacg
    13441 cggcgagatt tgccgtacca gttatcccac agccaggtca gcgtattgtt cagcccgtcg
    13501 cccagatatt gcggttgcac cacaatgcga tgcaggtttt tctgctggcg caaaaatgcc
    13561 atcgggtcag gaatacgata caggcgaacg tccacgccgc cgtactcttc catctgatag
    13621 cgccgataat cacgccccgg cgcttccagt cgcactttcg cctcttcact gctgctaaaa
    13681 ctgctgtcag caagcaaaaa gaatgttccc ccggcgggcg gcgcatagtt actggaagga
    13741 agcgaatcat cagcattagc aagccctgtt cccacaagag acagggacaa gcacgctgct
    13801 atcgcgccag aaaatttaaa cgataaatgc caatgaaatt gggattggaa tcgttgggta
    13861 tccatcgggt gtccttccat gtcataagtt gttgcagact gactgcgcgc attccgttgt
    13921 cagttttcgt ggcgcttccg gtgtggtaga tgacgtaacg ccccatccag accattaagt
    13981 gctgggcatc gccctgatcg aaaaaaatca tatcgccagg cagcgcctgg tttatgtcct
    14041 ggccaataaa ctggctgttg tactgaatca aattaatcgc ggtcacgtag gggccggttt
    14101 tcccgttccc ctgattccag ttttgcgcca gttgacgctg tccaggtgtt agcgtcatct
    14161 ctggcggcaa atactggcta gagaaaccgt tactttttag ccatttacta tcgtgaactt
    14221 tcagcgtctc gttcgccgca aatcgcacca ggcccgcaca atcctgctga taccagcgtg
    14281 gacttggccc ctggcggagc tgttcttgtg caatgcgtac aaaccaggcg cgaaacagcc
    14341 cggattgttc gacattcagc atttcgctat gggcaacaac acaacacagc cagcaaatca
    14401 gcgccagcag cccgtgcctc atagcggctg ccaggttatg ggtagccact gccaggcggc
    14461 accgggttcc atctgggcca gcttcatgac ataacgcggt tgttgagata aagcgtccag
    14521 cttcggcatt aataaagttt gtgcggcgtt ataaaaaacc ggttcgagat tcttcggcag
    14581 actggtcaac gtttcgttac gcagcagttt cgccacgcct tgtggattga tatagagcgg
    14641 aacgatgcca tcagtgggta ttacatccac cattgccggg cggtttttat tcagtgtttg
    14701 cagggcgtta ttaaccagcg tgtcatcgag ggagaaaagc agcgttttgt tttgcatcgc
    14761 cagcgacacg cggaaaaaat aatccgacat taattgatcg ggttgcgccg cctgcgcttt
    14821 cggatactga ccgtatcggg aactcacttc gcgacgccag atttgcgctt cgccctgctg
    14881 agcctggctt atcggcaaaa caccttctgg cgctttgctt tcgtgcgcac caatattttg
    14941 cgtaaacagt ttccctggca attgcgcctg ttcggcagtg ccatcaaact gaccgacaaa
    15001 cagcggggtt tgcaattttg agtctgcata ccagcacagc cccgcggcac cgtctaacgc
    15061 cccattcaac ttgtcgtttt cctgactgat gtgcgaaagc atctcttcgg caataccgtg
    15121 tgaatacggc accaccacac agaagctggc cccggcaggc atactgttcc agaccggcgt
    15181 aaaatcgaaa ctggtatcta cgctggcgga ttcatcattt aacgccacaa aactgtgcca
    15241 gccgtcgtta cccatttcga agcgtacgcc agcaaaagaa ggcattaacc gctggtagcc
    15301 aaaacccagc aacctggcgc tgaccacgat gcgctggcgt actggcgttt tttcagcagc
    15361 acgctcttcc aggccaaagc ttgcttgcca gcgttttttg ccgctcaaca aatcacctgc
    15421 gatcgccgtg gcttcggtat cctgctgatc atctttaaac aacatatccg tgctggaaaa
    15481 caccagcatc ttgtcctgat aagtcgcgaa catcagggcg ttattgccgt tatagcgcaa
    15541 ctgataaaca ggaacagttt cactatttat cttgatgcta ctgatttccg ttttgcttaa
    15601 ctggctgtcg ctggtagcgg caaacaacaa tggctccagc agtttgctta aaccactgcg
    15661 ctggatcagc accatataat gtgaaagatg gccttgttta tcgtgccaca gcgctgcctg
    15721 cgcgggctga tctaagagtg acgaaaagag cttatctttc agcgtgagat cgtgttcata
    15781 gacaatacga cgaatgctgc cttcaatgcc cagacgatcg gcatgattct gataatagaa
    15841 aacgaaatct tcgctcagaa catcgtggag aaacggaatg gtgaggagat ctttgggaag
    15901 ctggctcaga gagtcgctgt cgagaaagag gtccggctca ttgagatcga tttgcagatt
    15961 gttgtgcacc accagcggcg acaacgtttt ttctggccca ctgccagcat attgcaacgc
    16021 ccagacgcca gcggaaagca gtgctattgc gccaaaacct acaagaccat agaaccgcca
    16081 gcctttcgcc tttttttcac cactcattgc cacattcctt gtgtatagcc agccattttt
    16141 tacgggcaca gccaaacttt accgtgccct aatacgacaa aagcccagac tttgcagcct
    16201 ggacttttca attcaaacaa gggagatagc tcccttttgg catgaagaag taaaattatt
    16261 cttcttctgg ctcgtcgtca acgtccactt ccggagcgat ttcatcgtcc ccttccgcgg
    16321 cactgccatc gatggtatcc agatcttcct cgtcaaccgg ttcagcaaca cgttgcagac
    16381 ccactacgtt ttcatcttcc gcagtacgga tgaggatcac gccctgggtg ttacggccta
    16441 cgatgctgat ttccgaaacg cgagtacgta ccagcgtacc ggcatcggtg atcatcatga
    16501 tctggtcgca gtcatctacc tgtaccgcgc caactactaa accattacgt tcagtaacct
    16561 taatggagat aacccctttc gtcgcacgcg acttggttgg gtattccgcc actgcggtac
    16621 gtttaccgta accgttttgc gttgcggtga ggattgcgcc atcgccacga ggcacgatca
    16681 gagagacgac tttatcgcct tcacctaagc gaataccgcg aacaccggtg gtgttgcagc
    16741 ccatcgcacg gacagaagac tctttaaagc gcaccacttt accttcagcg gagaacagca
    16801 ttacttcgtc ttcgccgctg gtcaggtcaa cgccgatcag ctcatcgccg tcaaccagtt
    16861 tgatcgccac tttaccggcg gtacgcagac ggttgaactc ggtgaggaca gttttcttca
    16921 cggtaccgtt agcggtcgcc atgaagactt tcacgccttc ttcaaactcg gtcactggca
    16981 ggatcgcagt gatacgttcg tcctgctcca gcggcagcag gttgacgatc ggacgaccgc
    17041 gcgcgccacg agtggcttcc ggcaactgat aaactttcat cgaatagacg cgaccacggc
    17101 tggagaagca cagaatatgg tcgtgagtgt tcgccaccag cagtcggtcg ataaagtctt
    17161 cttctttaat acgtgcggca gatttacctt tcccgccacg acgctgcgct tcgtattcag
    17221 aaagcggctg atacttaacg tagccctggt gagagagcgt cactaccaca tcttcctgag
    17281 tgatcagatc ttccaggtta atgtctgcgc tgttggcggt gatttcagta cgacgtttgt
    17341 caccgaactg ttcacgaacc agctccagct cttcacggat cacttccatc aaacgatcgg
    17401 cgctaccaag aatacgcaac agttccgcga tctgatccag cagctctttg tattcgtcga
    17461 gcagtttttc gtgctcaagg ccggtcagtt tctgcaaacg cagatccaga atcgcctgag
    17521 cttgctgttc ggtcaggtag tacagaccat cacgcacgcc gaactctggc tccagccatt
    17581 ccggacgcgc agcatcgtcg ccagcacgtt cgagcatcgc ggcaacgttg cccagctgcc
    17641 acggattagc aaccagcgca gttttcgctt ctgcaggcgt cggcgcatga cggatcagtt
    17701 cgatgatcgg gtcgatgttc gccagcgcca cggctaatgc ttcaaggata tgagcacgat
    17761 cgcgagcttt acgcagttcg aaaatagtac gacgggtcac cacttcacgg cggtgacgaa
    17821 caaacgccgc gatgatgtct ttcaagttca tgatcttcgg ctgaccatgg tgcaatgcca
    17881 ccatgttgat accgaaagaa acctgcaact gggtctggga gtagaggttg ttgagcacaa
    17941 cttcaccgac cgcatcgcgt ttcacttcaa tcacgatgcg cataccgtct ttgtcagact
    18001 cgtcacgcag cgcgctgatg ccttccacgc gtttttcttt taccagttcc gcaatcttct
    18061 cgatcaggcg cgctttgttt acctgatacg gaatttcgtg gacgataatg gtttcacgac
    18121 cggttttggc gtcaacttcc acttctgcgc gagcgcggat atacaccttg ccgcgaccgg
    18181 tacggtaagc ttcttcaata ccgcgacgac cgttaatgat tgccgccgtc gggaagtccg
    18241 gccccgggat gtgttccatc agcccttcaa tgctgatgtc ttcatcatcg atatacgcca
    18301 gacaaccgtt gatgacttcc gtcaggttgt gcggcgggat gttggttgcc atacctacgg
    18361 cgataccgga agaaccgttc accagcaggt taggaatttt ggttggcatg acgtcgggaa
    18421 ttttttccgt gccgtcatag ttatcaacga aatcgaccgt ctctttttcg agatcggcca
    18481 tcagttcatg ggcaattttc gccagacgga tttccgtata acgcattgcc gccgcagagt
    18541 cgccgtcgat ggaaccgaag ttaccctgac cgtctaccag catgtaacgc agcgagaatg
    18601 gctgcgccat acggacgatc gtgtcataaa ccgccgagtc accatgggga tggtatttac
    18661 cgattacgtc accaacgaca cgggcagatt ttttataggc tttgttccag tcattgccta
    18721 gtacgttcat ggcgtaaagt acgcgacggt gtaccggctt caggccatct cggacatctg
    18781 gcagcgcacg gccaacaatg accgacatcg cataatccag ataggagctc ttcagctctt
    18841 cctcaatgtt gaccggtgta atttctctcg caaggtcgct catctaaccg ctatccctct
    18901 actgtatccc ggattcaaag gtcgcaaatt ataacacagc cgcgcagttt gaggtaaacc
    18961 tatacgcttt attcacatcc aatgcctgat atactcgttt gtcttgccaa ttacggagta
    19021 gaagtgccaa tgaatgccga aaaatcgccg gaaaaccata acgtagacca cgaagagatc
    19081 gctaaatttg aagccgtcgc ctcccgctgg tgggatcttg aaggtgagtt caaaccgttg
    19141 caccgcatta acccgctgcg tctgggctat attgccgagc gtgctggcgg tttatttggc
    19201 aaaaaggtgc tcgatgtcgg ttgtggcggc ggcattctgg ccgagagtat ggcgcgcgaa
    19261 ggcgcgacgg tgaccggtct ggatatgggc tttgagccat tgcaggtggc aaaactgcac
    19321 gcgctggaaa gcggcattca ggtggattat gtgcaggaaa ccgtggaaga gcacgcggca
    19381 aaacatgccg ggcagtatga tgtggtgacc tgcatggaga tgctggagca cgtccccgat
    19441 ccgcagtcag tggtcagagc ctgtgcgcaa ctggtgaaac caggcggcga tgtctttttc
    19501 tcgacgctta accgcaacgg caagtcatgg ctgatggcgg tggttggcgc ggaatatatt
    19561 ttgcgcatgg tgcccaaagg cacgcatgat gtgaagaagt ttattaaacc ggcagaactg
    19621 cttggctggg tagatcagac aagccttaaa gagcgacata taaccgggct acattacaac
    19681 ccgctcacta atacttttaa actcggcccc ggcgtggatg tgaactatat gctgcacacg
    19741 cagaataagt gaggttgttg tttggccgag ccaatgcctg atgcgacgct tgccgcgtct
    19801 tatcaggcct acaaatgctc cccgtaggcc ggataaggcg tttacgccgc atccggcaac
    19861 cgtgccgact agacagtgat taccatttca ccgtcatcga caaaaaccct gccatctggg
    19921 caaaatcatc actccctttc tgccacgcca cgctgccgcg cagggacact cgctgactga
    19981 tattgcccgt gactcccact tttatttcac cccgttgctt caccgcatcg tcactgatag
    20041 tgctgccatc ctcttcaatt tccgtcgaat ggggattgtg ataataattc agatctaatg
    20101 ttggtatgac atgaacagcg gtacgccatt cgctgtgtaa acccagccgc atctgaatat
    20161 catcaccctg cgattgtgac acgcgcgcac ggttagcggc ggtaaaatca tcctgctgca
    20221 cgccctgata aatcacctgc gcctgcggtt caatcaccac accacgcccc ggtaaccact
    20281 gatacccggc ttccagcgag gcgataatcc ccgacgagtg gtaatgatct gtgccatcgt
    20341 cttgttcgga aacatcattg ttaaaccacg cgtattgcag ccagctatcc agccaggccc
    20401 cctgcttctg attaccgtgc tgataccagc ttgaggtcag cccaacggca taaccgtggt
    20461 tctggttatc ggcgcgagtt ccggtcatat tcgagcggct ctcgccctga ttatcactgt
    20521 agccaccaac cgccccaagc atccactcgc catcatcgcc ccagcgcccg ctaaacaggt
    20581 caccgctaag ctgcaccgta gaagtgtctt catgctgagc cagttgccct gccgccgtgt
    20641 aatgataacg tccgccgata acacgtaaat tcagcgtctg accattgcca cctgcatgat
    20701 cgcgtcgctc catcataaac gcctgatttg ccgcccgcag attattgaga taaccgccaa
    20761 ctttggcatt cagcaccggc tggtaagcgg ggattggatc gggtgttggg tcaggatcgg
    20821 gtgttgggtc agggtctgga tcaggtggca atggtggcgt tacttcttgc gatcgcagat
    20881 accagtcgtt gttatcttcc accagcgtgt agtcatatgc tcccatattg acgtagccgc
    20941 tgcctgccag actgaacttc gcattgtttt gaaactgcgt gggatcggct gcgaaatcaa
    21001 ccactttaat gcctgtcgat gtcggctcac caatccctgt aatggagtta accaccacag
    21061 ttgtgttgcc agcagtatta ccgttcaaca ccaattgatc gcttaccgag tcatcgccgt
    21121 ttaattcgct atcgagcagt agcgtaccgc cgccggtata atcaccgttt accgtcagcg
    21181 tatcgccagc gacgccattt tgcaggctaa ccgcaccgga cgtattggtc agattgccat
    21241 taaccgtcac gtcggaaaga taggtcaggc tgtcagtttc aaggttggta ttaagctcac
    21301 ctgtcaacgt cacgccatcg gtggcattca ccagcgtccc atcgccgtta aacaatgagg
    21361 cattaagggc gacggaagta tcctgcccgg tcagacgcaa aaccgtacca tcgctgatat
    21421 cgatagtgcc gctggaagta gcatcgatag actgaatatc ctgatccgcg ccagtaacga
    21481 acgtcgcccc atcaccgacc cacagtgacg aagcataagg aaagatatcc gcaacatcgc
    21541 ctttcagggt gccttcttcg acacgtactg ccgactgcgt tgtaccgctg gcggtcagct
    21601 ccagcgtacc cgcccccgtt ttagtcaatg tgctaccctc atcctgatgc tgcccgctgc
    21661 tgtcagccat cagtgcgccc cactgcgtgt ctacccccgc atcaactgcc acttcaccgt
    21721 cggcgcgcat ttcaatatca cgcccatgct cagtcgtcgt agagcgagta tcgacattgg
    21781 cagtaatctc catcacgctt tcttgttgct tatcggtaaa gataacctgg cggttatagt
    21841 gggtatctcc aagctgcgag ttgtcgctca ccatcagcgt accggaggcg atttgtgttg
    21901 tgcccaaata actattattg tttgccagac ttacctgccc gtcgctcgca tcgaccacca
    21961 cgtagacgcc atctttcccg tcgccgccga tatgcaaatt gtcgccacct gcaagatcta
    22021 ccacatcctg actgccgata accgttccgc tgctgccact gacttcaagg ccatcattgt
    22081 atgatgtgcc gctctgccag gtggagaaat cagagagatc gagcacaccg ccgtttaaca
    22141 cgatcgactg cggatcgtcc tggagagcgg cgagatctgc cgcgtcgcct tccagcgaaa
    22201 gcaccgcacc atcatcaacg actatatcgc cggttagcgc catcgactgc gcccccgaca
    22261 gcacgtagct gccgttttgc gcaatggtga gctgaccagc accttcgatg gtgccagcaa
    22321 aactgccttg attaacagta acattgccac cagcatcgat atttaaagtg ccattctgaa
    22381 agcccgtcaa tgagtgcaca aaggtttgtt gggtggagcc aacgtttagc tctgcctgat
    22441 tctggtactt atcaatactc cctatcgtca gaccgtagca gtcttgcgga tcgtcctggc
    22501 aatgcgtatc gccgacattc atcagggagt tgctgcggcc cagggtaact tcaccgtttt
    22561 caatctgcat ctcaccagta aagtcattgt tatctgcatt aagtaccaga tcgccggaac
    22621 ctgttttggt gatcaacccg gtaccagcaa tagagtcaac agctccgtca ttctctgtat
    22681 tgccaataac cagcgttttt ccgtcggcaa tatcaaaggt aacttcactt aagccgagat
    22741 acataaagcc acccgccgca gtggaaggac catctccata gcctgctgcg ctgttgttct
    22801 catccactaa cacgccgccg ttctggctgt agctgtcatc aacagaaata tcaataagat
    22861 agggagccgt cgcgctattg gtatatatcg ccccgccata accttcggca gtgttatttg
    22921 taaaggcagt gttatttatt atcgtataac ctgaaggatg cttgctgacg ctattattat
    22981 cggtaacatc gattgccccg ccatcgccat cactgtaact tgttgatgta tatgcctggt
    23041 tgttatcaaa aataacatca cttaaataga cgtcattatt aatggtataa attgcgccac
    23101 ctttgccatc attagcgata ttattgcgaa acatggcgtt agtgacacgt aaatcgacgg
    23161 caccagtatc attagtacca gaagaataga ttgcgccacc atagccgcct gcgacgttac
    23221 cagaaaaaat aacatcagta agattcagcg ttgagttttc tttagcaaat atggccccgc
    23281 cgttattata ttctcctgta acggtgttat tggcaaacag agtcattcca gtttcatttt
    23341 ctggtaatag tgaaaattcc gctccttgtt gcaaaaatac ggctcctccg ctggcgttat
    23401 tggtcatatc cgaaaaaacc agccattgcc catcggcgac actccagtcc tgcgtaatgc
    23461 ctgaaaggct ttgcctgctg gcgtgacaac tcgctttgat atcatatccc tggcataaat
    23521 caatggccgc cgcgaaaccg ttagcagaga aaagagatgc aatcattgac gggagtagag
    23581 ataaatactc cttgcgtaga aagataatcc gcatattcat ctaaccatca ttttatataa
    23641 gacggcatat ttaaacgcat tatacaatat gggttatctt tcttttgtaa attgtcc
//
LOCUS       DAZFRN010000071        22889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFRN010000071 DAZFRN010000000
VERSION     DAZFRN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.071"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            1..755
                     /locus_tag="ACHKUB_002683"
                     /pseudo
     CDS             1..755
                     /locus_tag="ACHKUB_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293189.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            875..3991
                     /locus_tag="ACHKUB_002684"
     CDS             875..3991
                     /locus_tag="ACHKUB_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO6655676.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEKAAN
                     KSATYKPLRIATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSHFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYTEYMEGF
                     TDAATGEAKRGFMTVVSELEQRFPDPTSIESEKEKKDFVKLFGEYLRAENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKEEHYVDDEKFAELQTIRLPADRKIQDYRSAYNDIRDW
                     QRREKEAEKKEKSTTDWGDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKGEMIEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDAFFTFAQREQQREAEALIKEE
                     NLNEEAAKRYIRTSLKREYATENGTELNETLPRLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGKI"
     gene            complement(3966..4157)
                     /locus_tag="ACHKUB_002685"
     CDS             complement(3966..4157)
                     /locus_tag="ACHKUB_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655677.1"
                     /translation="MGEISVKTGNNARNGGKNSLNRLIRCVCGKKIGPDPRNFNQRVS
                     LGRNDLLIRTFPKSFHPPP"
     gene            complement(4139..5119)
                     /locus_tag="ACHKUB_002686"
     CDS             complement(4139..5119)
                     /locus_tag="ACHKUB_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HFO6655678.1"
                     /translation="MSHQLTFADSEFSIKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVEATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQNPRKNKTAINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
     gene            5381..6899
                     /locus_tag="ACHKUB_002687"
                     /pseudo
     CDS             5381..6899
                     /locus_tag="ACHKUB_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
     gene            6889..7227
                     /locus_tag="ACHKUB_002688"
     CDS             6889..7227
                     /locus_tag="ACHKUB_002688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655679.1"
                     /translation="MARDSLYPVSVKAGKPILGMLPTGWQKLSLEKCLNIEARKAYIQ
                     DNQEYDLVTVKRSRGGVIRREHLKGKDISVKSQFYIKEGDFLISKRQIVHGANQWAGP
                     CRSRRTAGSP"
     gene            7176..7598
                     /locus_tag="ACHKUB_002689"
                     /pseudo
     CDS             7176..7598
                     /locus_tag="ACHKUB_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            7623..9074
                     /locus_tag="ACHKUB_002690"
     CDS             7623..9074
                     /locus_tag="ACHKUB_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO6655680.1"
                     /translation="MESINNQVQSVRQLLAIPQLAIPSYQRPYRWGSKNISDLFTDLA
                     THQDKSAYRLGSVVFHQHTDSEQGEMLDIVDGQQRTLTLMLTVKALIEVLDNENRSGK
                     EKSIRFQRQDLREQLQVLRGPIDAFMQRQRFPSRDSEFNLRRNYLELRRLVARPEFDE
                     QQIDFLLNRCQVVCFVLQDISEAFQFFDSQNARGRDLAPHDLLKAFHLREFAGHEANL
                     KAEAVAHWERLPSDELANLFALCLYRVRQWAEGKSARYFGKGEVDLFKGVNLDRVGHY
                     PYVESLRIAHHFVDEYNSQYQRKIDGQYMTFPFHLDQMIINGRRFFEMAEYYQTRVAA
                     IVAEESDSGKAQSATLLGETLTPMASKVLSTLGSYERRHRTGDRYVRAMFDCALIFYI
                     DKFGSQGLSLAIEKCFIWAYRCRIRQQVVQLATMDNYVLEHNLIRAIRDARLPGDLHG
                     FPLPSMSSRENKNNRNGAEDELVGLFREMKYYE"
     gene            9067..11109
                     /locus_tag="ACHKUB_002691"
     CDS             9067..11109
                     /locus_tag="ACHKUB_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO6655681.1"
                     /translation="MNDAGVRPFSIRALLEDRARYLVPMYQRNYAWGEGEITQLLQDV
                     LDYQQKLVSGKGPQTYYIGTLVVFARDDGSFEVIDGQQRFTTLSLLANWLKHHAIDSV
                     DMSWYQTINLAFESRPISSHTFERLWQGVAPDDLRGSTFNEGLVNGFELIGKAMADLG
                     LVGAKLTAFCDYLFKHVQISRIEVPKDTDLNHFFEAMNNRGEQLEKHEVIKARLMETL
                     NQIPEEEHRRQSIHILTRVWDACANMERYIQYGFTPQERHRLFGESDWGQFVPRDFAH
                     LLDLLGSPNTADAADKSRAAAGSQGRTLLAILQDDKLDGEQTVEEESAGSERFNSVIN
                     FSNFLLHVLRLVSRDPGDTEGVPLDDKQLVDQFELRVIRQPDPVAAVQRFIYGLLKSK
                     YLFDQFIIKREFADGKDSWSLKRLHWYSEKSVSYINTFDKDENENGFSGVNRRVLMLL
                     SAFHVSTPTLVYKHWLNGALRYLFDNCHPDQPVDAGAYLSYLESQARRFVFQRFLAPG
                     EGASYYQMLYLDNALLPAINVDESWHKVITSKLRFGHIENNFVFNFLDYLLWVRDRNS
                     DKVAGSFEFTFRSSVEHFSPQHPMDGYKPVEQSALHSFGNLCLISHSKNSRLSNFQPQ
                     QKQEHFEASLANNQTDSLKLLAMIRLMKDKGRWLEDEIAVHQQDMLQVLLSNQIQQ"
     gene            11293..17028
                     /gene="hhe"
                     /locus_tag="ACHKUB_002692"
     CDS             11293..17028
                     /gene="hhe"
                     /locus_tag="ACHKUB_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing anti-phage protein
                     Hhe"
                     /protein_id="HFO6655682.1"
                     /translation="MSTVDTSTAEELNQGGSDFILTSLEAMRKKLLDLTSRNRLLNFP
                     ITQKGSSLRIVDELPEQLYETLCSEIPMEFAPVPDPTRAQLLEHGYLKVGPDGKDIQL
                     RAHPSAKDWAHVLGIRTDFDLPDSHKTVVSNSDRELLEKAHQFILQYAQGQNGKLTGI
                     RSEYVNQGIALSALKEACCLAGYEGLEDFERQAKAGNEISISSSNPSHDDNRIQALLY
                     PNELEACLRAIYGKAQTALEESGANILYLALGFLEWYESDSSEKARYAPLFTIPVRCE
                     RGKLDPKDGLYKFQLYYTGEDILPNLSLKEKLQADFGLALPLFNEEETPESYFASVKK
                     VVEQHKPKWSVKRYGALSLLNFGKMMMYLDLDPARWPCDKRNILSHEVIRRFFTSQSC
                     GQENSGLPGGFGQHEYCIDSYPDIHDKVPLIDDADSSQHSALIDAIRGQNLVIEGPPG
                     SGKSQTITNLIAAALFNGKKVLFVAEKMAALEVVKRRLDRAGLGQFCLELHSHKTHKR
                     KVLDDINARLVSQATMPTMEEIDAQILRYEDLKQQLNEYAALINNQWAQTGKTIHQIL
                     SGATRYRHKLDIDATALHIENLSGKQLDKVTQLRLRDQIVEFSRIYKEVREQVGANAE
                     IYEHPWSGVNNTQIQLFDSARIVDLLQTWQTSIIDFQHSYQEYVDKWALEGESLNTLQ
                     YIEQLVEDQSNLPVLCGSEHFPALSELDSPDAIARVRHYLDRFELLQGHYVALSQVIE
                     PQKLRLLEQGQSCDFPREELEKYGAAEDFTLRDLVRWLESIQSIHDELSSIYAQLNDF
                     KNALPDGIASYIDDSQAGLLFCSELLSILGALPTELIRVRDPLFDDDDIDAVLRDLMC
                     QIETLRPLRDGLSTLYQLDQLPSQEMLAHAVAVIQQGGLFAWFKSDWRSAKALLMAQS
                     RKPDTKFAELKRCSADLLKYSELLQRFEQSDFGNQLGNAFRGLDTDCEQLMLLRDWYK
                     KVRACYGIGFGKRVAIGSGLFNLDGEIIKGVHLIEKSQISSRLMTLVKRVEHEAKLLP
                     RISSLLEEHASWLGEQGVLMQSYRQVRNTLIALQGWFINPDISLEQMTHSSEILQNIN
                     DLQISLENDSLQLGAFLQLTPLACGAYKNNQLTLDTINDTLNFAEQLVDKINCVSLAT
                     QIRHLASGSDYDLLCRDGGEIVSKWNEQIKNAELYALETKLERSQWLKSTDGSLNTLI
                     ERNERAIQQPRWLNGWVNFIRCYEQMHENGLQRIWSAVLAGSLPIEKVELGLALAIHD
                     QLAREVIHIHPELMRVSGSQRNALQKSFKDYDKKLIELQRQRIAAKIACRNIPEGNSG
                     GKKSEYTELALIKNELGKKTRHIPIRQLVNRACNALVAIKPCFMMGPMSAAHYLEPGR
                     MEFDLVVMDEASQVKPEDALGVIARGKQLVVVGDPKQLPPTSFFDRSADGEDDDDATA
                     LSDTDSILDAALPLFPMRRLRWHYRSRHEKLIAYSNRHFYNSDLVIFPSPNAESPEYG
                     IKFTYVSKGRFSNQHNIEEAQAVAEAVLHHAQHRPGESLGVVAMSSKQRDQIERAIDE
                     LRRNRPEFNDAIDGLYAMEEPLFVKNLENVQGDERDVIFISFTYGPSEHGGKVYQRFG
                     PINSDVGWRRLNVLFTRSKKRMHVFSSMRSEDVLTSETSKLGVISLKGFLQFAESGKL
                     DSLTTHTGRAPDSDFEVAVMEALNHAGFECEPQVGVAGFFIDLAVKDPGCPGRYLMGI
                     ECDGAAYHSAKSARDRDRLRQEVLERLGWRISRIWSTDWFSNPDEVLSPIIRKLHELK
                     TLAPDVVVPSYEYVETIESSAEVASDSIDSLMPNLGLKEQLKYFATHVIEVELPNVDA
                     DRRLLRPAMLEALLEHQPLSRSEFVERIPHYLRQATDVYEAQRFLDRVLALIDGAEAE
                     ANDAAFESELA"
     gene            complement(17249..17707)
                     /locus_tag="ACHKUB_002693"
                     /pseudo
     CDS             complement(17249..17707)
                     /locus_tag="ACHKUB_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(17960..19573)
                     /locus_tag="ACHKUB_002694"
     CDS             complement(17960..19573)
                     /locus_tag="ACHKUB_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFO6655683.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(19604..19954)
                     /gene="tnpB"
                     /locus_tag="ACHKUB_002695"
     CDS             complement(19604..19954)
                     /gene="tnpB"
                     /locus_tag="ACHKUB_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6655684.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            complement(19951..20376)
                     /locus_tag="ACHKUB_002696"
     CDS             complement(19951..20376)
                     /locus_tag="ACHKUB_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6655685.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAVIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            complement(20464..20694)
                     /locus_tag="ACHKUB_002697"
     CDS             complement(20464..20694)
                     /locus_tag="ACHKUB_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655686.1"
                     /translation="MKLFIFSLFVIVTSMVSGIAIAELSYFILLIIKYLAYGEVDFRL
                     SEVLRGLRMGCVGGGILGFGIVLFRFLGIKGF"
     gene            complement(20691..21371)
                     /locus_tag="ACHKUB_002698"
                     /pseudo
     CDS             complement(20691..21371)
                     /locus_tag="ACHKUB_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21410..21658)
                     /locus_tag="ACHKUB_002699"
     CDS             complement(21410..21658)
                     /locus_tag="ACHKUB_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655687.1"
                     /translation="MISYLKLFIYACVFSIGVCLVLGAGVSSLIYFSKGYWFFPLHQI
                     KRAFVFGTITGTAITVAAVVFKLIDAFNARKKPPSDTE"
     gene            complement(21651..22388)
                     /locus_tag="ACHKUB_002700"
     CDS             complement(21651..22388)
                     /locus_tag="ACHKUB_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFO6655688.1"
                     /translation="MLLYGYLIFFFPKELIWFFVDSYVVENNALSFGDGFESNAAAAT
                     SWNKYAVGNNLTPEQTQAGLDKIAKGDMSDSINITKVIVDGHQDGVMIAGAWYLGPAA
                     SAGKVLGGGLLGLAANSGYQFYDLNQPQNANKSWDYLGSATSFTTGMMAPGRGVLANT
                     GIAMGGAFFTDGPNTASLAGAGIGAGLGGAFGKYAPVGVDKVLNANNIPNFVYDAIGG
                     VGREFINGFTKEALNNPSEEKSGAKND"
     gene            complement(22324..22818)
                     /locus_tag="ACHKUB_002701"
     CDS             complement(22324..22818)
                     /locus_tag="ACHKUB_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO6655689.1"
                     /translation="MECIKCKMSEANKESGLCDACENEELSKINGLLYLPALGLILGV
                     IGSTIELYSFTITAFNYFNETGFLSYYAMGAFALLICGFIVSLYATWLFFRRKKGTRK
                     AMIAYYATGLIIALYLTLLPAILFDVQLDSDNIRLLVSGIFGVVIWIPYFLFSKRINM
                     VFCR"
BASE COUNT         6015 a         5358 c         5559 g         5957 t
ORIGIN      
        1 gttccaatcc cctgcccgga caatccggaa aaatcccttg ccatccagtc tgaaatcgtg
       61 cgaattctgg acaaattcag cgccctgaca gcagagctta acatgcgtaa aaaacaatac
      121 aactactatc tagataagtt attgaatttc aaagatattg cgcggggggt aattacatcc
      181 ttccggacgc catggatggt attcggattg agtggaagac tttgggggaa gtggctgtta
      241 taggaacagg taaccatgat acgcaagatg ctatagaaca tggcaaatat attttttatg
      301 cacgtggacg tgaaccatta aagttaaatg tatttgattt tgatgaaaca gcaatcatta
      361 cggctggaga tggtgccggg gtggggaagg tatttcatta cgctaaaggt aaatatgctt
      421 tgcatcagag agcatatcgt attgtcccaa atgcatttat gaatccgcga tttgtatatc
      481 actatataac agcatatttt tttacctata ttcaaaaagc atcagtaagt tcatcggtta
      541 cctcacttcg tcgaccaatg tttttaaaat tcccaatacc tgtaccacct tcagaagaac
      601 aagctcgcat tgtcgaaata ctcgataaat tcgacaccct gaccaactcc atcaccgaag
      661 gtctcccgcg tgaaatcgag ttgcgccaga aacaatacga gtactatcgt gatttactgt
      721 tcagtttccc gaaacctgaa accgtcagta actaattgac aattgctacc gaccgggcca
      781 ccttaacatc cggtcagtat ataaaccatt tttacacgcc ggaagtcacc cttaactccc
      841 ttccggcccc gccagacggc acaaaggatg cgctatgatt cttcagacac acaccattgc
      901 tgaaaccagt aactttatcg ttcttgataa gtacatcaag gctgagcaaa caggcgacag
      961 ctaccagagt gaatcggacc tggaacgtga actgattcag gacctgcaga atcagggcta
     1021 tgaatttatt tccgtaaaat cacagtcggc gatgctggct aatgtccggg aacagcttca
     1081 gagcctcaat ggcgtggtgt ttaatgacag cgaatggcgg cgtttcacgg agcagtatct
     1141 ggacaaccct ggcgatggca ttctggataa gacccgtaaa atccatatcg actatatctg
     1201 tgactttatt tttgacaacg ggcgtcttga gaatatctat ctgatagata aaaagaatct
     1261 catgcgcaac aaagtgcaga ttatccggca gtttgaacag gcaggttctc acagtaaccg
     1321 ttatgacgtc actatcctgg ttaatggttt gccgctggtg caaatcgaac tgaaaaagcg
     1381 tggcgtggcg attcgtgagg ctttcaacca gatacatcgt tacagcaagg agagttttaa
     1441 cagcgaaaat tccctgttta agtatctgca actgttcgtc atttccaacg gtaccgatac
     1501 ccgttacttt gccaacacga caaagcgcga taaaaacagt tttgacttca ccatgaactg
     1561 ggcgaaatca gacaacacgc tgattaaaga cctcaaagat tttaccgcca cctttttcca
     1621 gaaacatacc ctgcttaatg ttctggtgaa ctacagcgtt tttgacagca gtcagacgct
     1681 actggtgatg cgaccgtacc agattgctgc caccgagcgc attctgtgga aaattaagag
     1741 ttcctttaca gcgaagaact ggtcaaaacc ggaaagcggt gggtatatct ggcacactac
     1801 cggttctggt aaaaccctca ccagctttaa agccgcgcgg ctggcaacag aactggactt
     1861 tattgataaa gttttctttg tggtcgacag gaaagacctc gattaccaga ctatgaagga
     1921 atatcagcgt ttttcgccag atagtgttaa cggctcggaa aataccgcag gtctaaaacg
     1981 aaatctggat aaggacgata acaaaattat cgtcaccact attcagaaac ttaataacct
     2041 gatgaaagca gaaagcgacc tgcctgtata caatcagcaa gtggtgttta tatttgatga
     2101 atgtcaccgc agccagtttg gagaagcgca gaaaaacctg aagaagaaat tcaaacgcta
     2161 ttatcagttt ggttttaccg gcacccctat tttcccggaa aacgctttag gttcagaaac
     2221 gaccgccagc gtatttggtc gtgaattgca ttcgtatgta attaccgatg cgattcgtga
     2281 cgaaaaagtg ctcaaattca aggtggacta caacgatgtg cggccacagt ttaagtcttt
     2341 agagacagaa actgacgaga aaaaactaag tgcggctgaa aatcagcagg cgtttcttca
     2401 tcccatgcgt attcaggaaa tcacgcaata tattctgaat aatttccgcc agaaaactca
     2461 ccgtaccttc cctggctcaa aaggttttaa tgctatgttg gcagtgagca gcgtggatgc
     2521 cgcgaaagcc tattatgcga cgtttaaacg gttacaagaa aaagccgcta ataaatcggc
     2581 tacctataaa ccgctgcgta ttgcgacaat cttctccttt gccgccaatg aagaacaaaa
     2641 tgccattggt gaaatttccg atgaaacttt tgataccagc gcaatggaca gcagtgctaa
     2701 agagtttctg gacgctgcaa ttcgtgaata taacagccat tttaaaacta actttagcac
     2761 cgacagtaac ggttttcaga actactatcg tgatttagcc cagcgggtta aaaatcagga
     2821 tatcgatctg ctaattgtcg tggggatgtt cttaaccggc ttcgatgctc caacattgaa
     2881 cacgctattc gtcgataaaa acttacgtta tcacggcctg atgcaggcat tttcccgtac
     2941 caaccgcatt tatgacgcta caaaaacctt cggtaatatc gtcacgttcc gggatctgga
     3001 acgctcaacc attgatgcca taacgctgtt tggtgacaaa aacaccaaaa atgtggtgtt
     3061 agaaaagagt tatacagagt atatggaagg ctttactgat gctgctacag gtgaagctaa
     3121 acgcggcttc atgacagtag tttcagaact ggaacaacgg ttccctgacc ctaccagtat
     3181 tgaaagtgaa aaagagaaga aagacttcgt taaactgttt ggcgaatacc tgcgtgccga
     3241 gaacatcctg caaaactatg atgaatttgc cacgctgaaa gccctgcaac aaatcgatct
     3301 tagcgatcct gttgcggtag aaaaattcaa agaagaacat tatgtggatg atgaaaagtt
     3361 cgctgaattg caaacgattc gtctccctgc tgatcgcaag attcaggatt atcgttctgc
     3421 ctataacgat attcgcgact ggcagcgccg tgagaaagaa gctgagaaaa aagagaaatc
     3481 aaccactgac tggggtgacg tagtttttga ggtcgatttg ctgaagtctc aggaaataaa
     3541 cctggattat atccttggac tgattttcga acacaacaga caaaataaag gcaagggcga
     3601 aatgatcgaa gaggtcaaac gcttaattcg ttcaagccta gggaaccgtg ctaaagaggg
     3661 cctggtggtc gattttattc agcaaacgaa cctggatgat ttaccggaca aagccagcat
     3721 cattgacgca ttctttacgt ttgctcaacg cgaacagcaa cgtgaagcag aagccttgat
     3781 caaagaagaa aatctcaatg aagaggcggc gaaacgctat attcgcacat ctttaaaacg
     3841 cgaatacgcc accgaaaatg gcacggaatt aaacgagaca ctgcccagac tcagtccttt
     3901 aaatccgcaa tataaaacga aaaaacagac tgtttttcaa aaaattagcg catttattga
     3961 aaaatttaag ggggtgggtg gaaagattta gggaaggtgc gaataagcag gtcatttctt
     4021 cccaagctga ctcgctgatt aaaatttcgc ggatctgggc cgattttttt cccgcaaaca
     4081 catcgaatca gcctatttag gctatttttt ccaccatttc tggcgttatt tccggttttt
     4141 actgagatct ctcccactga cgtatcattt ggtccacccg aaacagattg gccagggtga
     4201 ataacatcgc cagttggtta tcgtttttca gcagcccctt gtatctggct ttcacgaagc
     4261 cgaactgccg cttgatgatg cgaaacgggt gctccaccct ggcacggatg ctggctttca
     4321 tgtattcgat gttgatggcc gttttgttct tgcgcggatt ctgcttcaag gtttttacct
     4381 tgccgggacg ctcggcgatc agccagtcca catccacctc ggccagctcc tcgcgctgtg
     4441 gcgctccttg gtagccggca tcggctgaga caaattgctc ctctccatga agcagattac
     4501 ccagctgatt gaggtcatgc tcgttggccg cggtggtgac caggctgtgg gtcaggccac
     4561 tcttggcatc gacaccaatg tgggccttca tgccaaagtg ccactgattg cctttcttgg
     4621 tctgatgcat ctccggatcg cgttgctgct ctttgttctt ggtagagctg ggtgcctcaa
     4681 tgatggtggc ttccaccaaa gtgccttggg tcatcatgac gcctgcttcg gccagccagc
     4741 gattgatggt cttgaacaat tgacgggcca gttgatgctg ctcgagcagg tggcggaaat
     4801 tcatgatggt ggtgcgatcc ggcagggcgc tatccaggga taatcgggca aacaggcgca
     4861 tggaggcgat ttcgtacagg gcatcttcca tggcaccgtc gctcaggttg taccaatgct
     4921 gcatgcagtg aatacgcagc atggtctcca gcggataggg ccgtcggcca ttgcccgcct
     4981 tgggataaaa cggctcgatg acagcggtca tattctgcca tggcagaatc tgctccatgc
     5041 gggagaggaa aatctctttt cgggtctgac ggcgcttaat gctgaattca ctatcggcga
     5101 aggtgagttg atggctcatg atgtccctct gggatgcgct ccggatgaat atgatgatct
     5161 catatcagga acttgttcgc accttcccta agataattgg tttttagcga gtgacgggct
     5221 aggttgtgtc agtctgtctc atgtcgatat tacggggatg atgcaaacct gaatgctgcg
     5281 atattggcgt gtcaggggag gcatcttacg gtatcattta ccgcaatttt taagatgcaa
     5341 tgccctgcat cgcaggcttt tcttcagacc aggatgattc atgaatgaca aaatcagtca
     5401 ggacacgatc aacaaagccc tgtgggccgc gtgtgatacg ttccgtggca ccatcagtgc
     5461 tgatacctat aaagacttta tccttaccat gctgttcctt aagtacattt cggatgtctg
     5521 gcaggatcat tacgatgaat ataaaaaaca gtatggtgat gcaccagaac tcattgaagc
     5581 catgatggcg aatgaacgtt tcgtattgcc gaaaagcgcc agtttctatg cgctgtatga
     5641 acgtcgtcat gaaccgggca atggtgagcg catcgaccag gctctgcatg cgattgaaga
     5701 agctaatggc accaaactga aagacgccgg gaaaagcgtg ttccaggata tttcgtttaa
     5761 caccgatcgc ctaggtgaag aaaaacagaa gaacaccatc ctgcgtcagc tgctggaaga
     5821 ctttgccggt caagatctta acctgaagcc gagccgtgtc ggtacgctgg acgttatcgg
     5881 taatgcctac gaatatctga tcaaaaactt tgctgccagc ggtgggcaga aagccgggga
     5941 attctatacc ccaccagaag tttcggacct gattgcagaa ctgctcgatc cgcaaccggg
     6001 tgataccatc tgcgatccag cctgtggttc cggttcatta ttgatgaagt gtgggcgtaa
     6061 aattgtctcc gggcatgata gtcgtaacta tgcattgttc ggtcaggaag cgatcggttc
     6121 tacgtggtcg ctggcgaaaa tgaatatgtt cctgcacggg gaagacaacc ataaaatcga
     6181 gtggggcgat accattcgta acccgaagct gctcgataaa aatggcgacc tgatgctgtt
     6241 tgatatcgtg accgctaacc caccgttcag cctcgataag tggggccatg acgaggccga
     6301 gaacgataag tttggccgtt ttcgtcgcgg tgtaccgccg aagactaaag gagattacgc
     6361 ttttatctcg catatgatcg aaaccctgaa accgggaact ggccgtatgg gcgtagttgt
     6421 gccacacggt gtgcttttcc gtggctccag cgaaggcaaa attcgccaga aactgatcga
     6481 cgaaaatttg ctggatgcgg taattggtct gccggagaaa ttgttctacg gggattcctg
     6541 cagcaattct gatttttaaa aagcagaaag tggatgataa ggtgctgttt atcgacgcca
     6601 gccgcgaatt caaggcgggt aaaaaccaga accagctcag tgaagaaaac atcgaaaaaa
     6661 tcgttaagac ctaccgcaat ggcgataacg tcgagaaata cgcttatctt gccagcctga
     6721 aagagattca ggacaacgac tataacctca atatcccgcg ctatgtcgat acctttgaag
     6781 aagaggatga gattgacctg ctggcggtgc gagcagaacg cgagcaactg aaggcggaat
     6841 tggcgaagct ggaaacggag atggctggat atttgaagga gttgggttat ggctcgtgat
     6901 tcgctttacc cagtgagcgt taaagctgga aaaccgatcc ttggaatgct acctacagga
     6961 tggcaaaagt tatcattaga aaagtgctta aatatagagg caagaaaggc atatatacaa
     7021 gataatcaag agtatgactt agttacagta aagcgctcca gaggtggcgt aataagacgt
     7081 gagcatctta aaggaaaaga tatctccgta aaaagtcaat tctatattaa agaaggcgac
     7141 tttctcatat caaaacgaca aattgtgcac ggtgcaaacc agtgggctgg gccatgtcgt
     7201 tctcgccgga cagcaggctc accatgaaag cactggaaat ggcatgggaa acccgtggta
     7261 agaccgtcgg ggtgatgttc cacagcgatc aaggcagtca ttatacgagc aggcagttcc
     7321 ggcagttact gtggcgatac cggatcaggc agagtatgag tcggcgtgga aactgctggg
     7381 ataacagccc aatggagcgc ttcttcagga gtctgaagaa cgaatgggtg ccggcgacgg
     7441 gctatgtaag cttcagcgat gcagctcacg caataacgga ctatatcgtt ggatattaca
     7501 gcgcactaag accgcacgaa tataatggtg ggttaccgcc aaacgaatca gaaaaccgat
     7561 actggaaaaa ctctaacgcg gtggccagtt tttgttgacc acttcatacc gcaggaggca
     7621 tgatggagag cattaataat caagtgcagt cggtacgcca gttgctggcc attccccaac
     7681 tggccatccc aagctatcag cgaccttacc gctggggctc caagaatatc tcggatctct
     7741 ttaccgattt ggcaacccat caggacaaga gcgcctaccg gctgggctcg gtggtgtttc
     7801 atcagcacac cgatagtgag cagggcgaga tgcttgatat cgtcgatggt cagcaacgca
     7861 ccctgaccct gatgttgacc gtcaaggcgc tgatcgaggt gctggataac gaaaatcgca
     7921 gtggcaaaga gaagagtatc cggtttcaac gtcaggatct gcgtgagcaa ttgcaggtct
     7981 tgcgtggacc tatcgatgcg tttatgcagc gccagcgctt ccccagccgg gattcggagt
     8041 tcaacctcag acgcaactat ctggagttgc gtcggctggt ggcccgccct gagtttgacg
     8101 agcagcaaat tgactttctg ctcaaccgct gtcaggtggt ctgctttgta ctgcaggata
     8161 tctcggaggc gttccagttc ttcgattcgc aaaatgcccg ggggcgcgat ctggctcctc
     8221 acgatctgct caaggcattt cacctgcgcg agtttgccgg tcatgaggcc aatctcaagg
     8281 ccgaggcggt cgcccattgg gagcgcctgc caagcgacga gctggccaat ctgttcgccc
     8341 tctgtctcta tcgggtacgc cagtgggccg agggcaagtc ggcccgctac tttggcaagg
     8401 gggaggtgga tctcttcaag ggggtcaacc tagatcgggt agggcattat ccttacgtgg
     8461 agtcgctgcg catcgcccac cactttgtcg atgagtacaa cagccagtac cagcgcaaga
     8521 ttgacggcca gtacatgacg tttccctttc atctcgacca gatgatcatc aatggtcggc
     8581 gctttttcga gatggccgag tactaccaga cgcgggtggc cgccatcgtg gccgaggagt
     8641 ctgattccgg caaggcgcaa tctgccaccc tgctcggcga aacgctgaca ccgatggcca
     8701 gcaaggtgct atcgaccctg ggcagctacg agcgtcgcca ccgcaccggg gatcgttatg
     8761 tgcgggccat gttcgactgc gccctgatct tctatatcga caagttcggc agccaagggc
     8821 tgtcactggc catcgagaag tgctttatct gggcctatcg ctgccgcatc cgccagcagg
     8881 tagtgcagtt ggcaaccatg gataactatg tgctggagca caatctgatc cgtgccattc
     8941 gtgatgctcg tctgccggga gatctgcacg gcttcccttt gcctagcatg agcagtcgag
     9001 agaacaagaa caatcgcaat ggtgccgaag atgagctggt cgggctgttt cgggagatga
     9061 aatattatga atgatgcagg cgttcgcccc ttttccattc gtgcactgct tgaagatcgc
     9121 gcccgctatc tggtgcccat gtaccagcgc aactatgcct ggggtgaggg agagatcacc
     9181 cagctgctgc aggatgtgct ggattatcag caaaaattgg tcagtggtaa ggggccgcaa
     9241 acctactata tcggcaccct ggtggtgttt gcccgggacg atggcagttt cgaggtaatc
     9301 gatggccagc agcgctttac taccctctct ctgctggcca attggctcaa acatcacgcg
     9361 atagattcgg tggatatgag ctggtatcag accatcaacc tcgcttttga gagtcgcccg
     9421 atatcgagcc ataccttcga gcgcttatgg cagggggttg cccccgacga tttgcggggg
     9481 agtaccttca atgaaggttt ggtgaacggt tttgagctga ttggcaaggc aatggcggat
     9541 cttggtttgg taggggctaa actgacggcc ttttgcgact acctcttcaa gcatgtgcag
     9601 atctctcgta tcgaagtacc caaggatacc gatctcaatc acttcttcga ggcgatgaac
     9661 aatcgcggcg agcagttgga aaagcatgaa gtgatcaagg cgcggctgat ggagacgctg
     9721 aaccagatac ccgaggaaga gcatcgtcgc caaagtattc atatcctgac ccgggtatgg
     9781 gatgcctgcg ccaatatgga gcgctatatc cagtacggct ttaccccgca agagcgccac
     9841 cgtctgtttg gtgagagcga ctggggtcag tttgtgcccc gtgattttgc ccatctgctt
     9901 gatctgctag gatccccaaa cacggccgac gctgccgata aaagccgtgc agctgccggt
     9961 agccaagggc gcaccttgtt ggccattttg caggatgaca agcttgatgg tgagcagacg
    10021 gtggaggagg agagcgcagg ctccgagcgc tttaacagcg tcatcaactt ctccaacttc
    10081 ttgctccatg tgctgcgact cgtgagccgc gacccgggtg atacggaggg ggtgccgctt
    10141 gatgataaac aactggtcga tcagtttgag ctgcgagtga taaggcaacc ggatccagta
    10201 gcggcagttc agcgttttat ctatggcctg ttaaagagca agtatctgtt tgatcagttc
    10261 atcatcaagc gcgagtttgc cgatggcaag gatagttgga gtctgaagcg gctgcactgg
    10321 tattcggaaa agagcgtcag ttacatcaat acctttgaca aggatgaaaa tgagaatggt
    10381 ttctctggtg ttaaccggcg ggtcctgatg ttgctgtcgg cgtttcatgt gtcgaccccg
    10441 accctggttt acaaacattg gctgaatggt gcactgagat atctgttcga caattgtcac
    10501 cctgatcaac cggtggatgc cggggcttat ctgtcctatc tggagagtca ggctcgccgc
    10561 tttgtcttcc agcgcttttt agcgcctggt gaaggggctt cctattacca gatgctctac
    10621 cttgataacg ccctgttgcc cgccatcaat gtggatgaga gttggcacaa ggtgattaca
    10681 tccaaacttc gcttcggtca tatcgagaac aactttgtct tcaacttcct cgactatctg
    10741 ctgtgggtac gagatcggaa tagcgacaaa gttgctggct cctttgagtt cactttccgc
    10801 agctcggtgg agcacttctc accccagcat ccgatggatg gctataaacc ggttgagcag
    10861 agtgcactcc attcgtttgg caacctttgc cttatcagcc acagcaagaa ctcgcgcttg
    10921 agtaacttcc agccgcaaca gaagcaggag cactttgaag caagccttgc caataaccag
    10981 actgatagtc ttaaattgct tgctatgatt cggctgatga aagataaagg tcgttggctg
    11041 gaagatgaga tagctgtgca tcaacaagat atgttgcagg tcttactatc caatcaaatt
    11101 cagcaataac ggattgaatc tgtttatgaa atttggctgc tatcaactaa tgggcgttaa
    11161 gttgattgta tgatctgatt gataaagaag ggggtaaaaa tctcctcttc tttgcagcag
    11221 tttactgcgg tctttttgtg atgcatcagc ataaaatgtt ttacttgtgg accctaagaa
    11281 atggagaaca ttatgtcgac tgtagatacc tctacagcag aggaactcaa tcaaggaggc
    11341 tcagatttta ttctgacttc cctcgaggct atgcgtaaga agttattgga ccttacgtct
    11401 cgaaatcgac ttttgaattt ccctatcact caaaaagggt cttcactacg tattgttgat
    11461 gaattaccag aacagcttta tgaaaccctt tgctcggaaa tcccgatgga atttgctcct
    11521 gtgcccgatc caactagagc gcagctgtta gagcatggct atctcaaagt tgggccagat
    11581 ggtaaagata tacagttaag agctcatcct agcgctaagg attgggcgca cgtcttagga
    11641 atccgtacag attttgattt accagatagc cataaaacgg ttgtttctaa ttcagataga
    11701 gagttgctgg aaaaagccca tcagtttatc ttgcaatatg cccaaggcca gaatggaaaa
    11761 ttaacaggga ttcgttctga atacgttaat caaggtatag ctttgtcagc gttgaaggag
    11821 gcgtgctgct tagcaggcta tgaagggctt gaggattttg aacgacaggc aaaggctggg
    11881 aatgagatta gtatatcttc ttccaatccc tctcatgacg ataatcggat acaggctctg
    11941 ctttatccaa atgaactgga agcttgtttg cgcgccatct atggtaaggc tcaaactgct
    12001 ttggaggaga gtggcgccaa catcttgtat ttggcgttag ggttccttga gtggtatgaa
    12061 agcgattcct ctgaaaaggc acgttatgca ccgttattta caattccggt gagatgtgaa
    12121 cgaggaaaat tagatccgaa ggatggtctt tacaagtttc aactttatta cacgggtgaa
    12181 gatattttgc ccaatctctc tttgaaggaa aaacttcagg ctgactttgg cctcgctctt
    12241 cctttgttca atgaagagga aactccagag tcttattttg cttcggtgaa gaaggttgta
    12301 gagcagcaca aacctaaatg gtctgtgaaa cgttatggtg cacttagctt gctcaatttt
    12361 ggcaagatga tgatgtatct tgacctcgat cctgcccgct ggccttgtga caagcgcaat
    12421 atattgtctc atgaagtaat tcgtcgcttt ttcaccagtc agagctgtgg tcaagagaat
    12481 tccggcttac ctggtggctt cggtcagcat gagtactgca tcgatagtta ccctgatatt
    12541 catgacaagg ttccactaat cgatgatgcg gatagctcgc agcacagtgc gttgatcgat
    12601 gctatccgtg gtcaaaactt agtcattgag ggccctcctg gtagtggcaa atcacaaacg
    12661 atcaccaact tgattgcagc agctctgttc aacggtaaga aagtcctgtt tgtggcagag
    12721 aagatggctg cactggaggt cgtcaaacgt cgcttggatc gtgcggggct aggtcaattt
    12781 tgcttagagt tgcacagtca taaaactcat aagcgcaagg tgctggatga tattaatgct
    12841 cgcttggtga gtcaggcgac catgcctact atggaagaga ttgatgctca gattttgcgt
    12901 tatgaagatc ttaagcagca gctcaatgaa tatgccgcat tgatcaataa ccaatgggcg
    12961 caaacaggca aaacgatcca tcagattttg agtggtgcaa cccgttatcg tcacaaatta
    13021 gatattgatg caacagcact tcatatcgaa aacctttccg ggaagcagtt ggataaagtg
    13081 acccaattac ggctgcgtga ccaaatagta gagtttagcc gcatctacaa agaggttcgt
    13141 gagcaggtgg gggctaatgc agaaatatat gagcaccctt ggagcggtgt gaataacaca
    13201 caaattcaat tgtttgacag cgctcgtata gtcgatttgc tacaaacttg gcagacatca
    13261 attatcgact ttcaacatag ctatcaagaa tatgtagata agtgggcgtt agaaggcgaa
    13321 agccttaata cgcttcaata tattgagcaa ttggtagaag atcagtcgaa tcttccagtg
    13381 ttgtgtggtt cagagcattt cccagcactt agtgagctag attcacccga tgccattgca
    13441 cgggtgcgtc actatttaga taggttcgag ttgctacaag gtcattatgt ggccttgagc
    13501 caggttatcg agcctcaaaa gctacgactt ttagaacaag gacaatcgtg tgactttcct
    13561 cgtgaagagc tggaaaaata tggtgcagca gaggatttca ctttacgtga tttggtcagg
    13621 tggcttgaat ccatccaatc aattcatgat gagttatcat ctatttatgc gcaattaaac
    13681 gatttcaaaa atgctttgcc agatggtatt gcttcgtata tcgatgattc gcaagctgga
    13741 ttgctattct gctctgagtt gttgtcgatt ctgggtgctt taccgactga gcttattaga
    13801 gttcgagatc ctctttttga tgatgatgat atcgatgcag tattgcgcga cttaatgtgt
    13861 caaatcgaaa cattgcgtcc tttaagagat ggtctatcta ctttgtatca attggaccag
    13921 ttgccttccc aagagatgct cgcgcatgcc gttgctgtta tccagcaagg gggattattt
    13981 gcatggttta agagtgattg gcgtagtgcc aaggcactgc tcatggcgca atctcgtaag
    14041 cctgacacta agtttgctga gttaaaacgc tgctcagctg atttgctcaa gtattcggag
    14101 ctgttacaac ggtttgaaca aagtgacttt ggtaatcaac ttggtaatgc attccgaggg
    14161 ttggacaccg actgtgaaca actcatgtta ttgcgtgatt ggtacaagaa ggtccgagct
    14221 tgttacggga taggttttgg aaagcgagtt gcgataggct ctggattatt taacctagat
    14281 ggtgagatta tcaaaggtgt gcatttaatc gagaaatcgc agattagctc aagattaatg
    14341 actttggtta aacgggtcga gcacgaggct aagttattac cgcgtatttc tagcttgttg
    14401 gaagaacatg catcttggtt aggtgagcaa ggtgtattga tgcaatctta ccgacaggtg
    14461 cggaatactc tcattgcctt gcagggatgg tttatcaatc cagatatatc attagagcag
    14521 atgactcatt cctccgagat tttgcaaaac ataaacgatc ttcagatatc ccttgaaaat
    14581 gactcgttac agttaggggc gtttttacaa ttaaccccat tggcttgcgg tgcgtataaa
    14641 aataatcaac tgacgttaga cactattaac gacacgctga attttgccga gcaactggtt
    14701 gataagataa attgcgtatc cttggctacc cagatcagac atttggctag tggtagtgat
    14761 tacgatttac tatgtcgtga tggtggggaa atagtttcga aatggaatga acagattaaa
    14821 aatgctgagt tatatgcgct agaaacaaag ttagagcgga gtcagtggct caagtcgact
    14881 gatggttctc ttaatacatt aatcgagcgc aacgaaagag caatacagca accccgttgg
    14941 ttgaacgggt gggttaactt tattcgttgt tacgagcaga tgcatgaaaa tggattgcag
    15001 cgaatctgga gtgctgtact tgcgggctcg ctcccgattg aaaaagttga attgggttta
    15061 gcattagcaa ttcatgacca gctggcgcgg gaggttattc acatccaccc tgaattgatg
    15121 agagtttccg gctcacagcg caatgctttg cagaagtcat ttaaagatta cgacaaaaaa
    15181 ctgattgaat tacaacgtca gcggattgca gcaaaaattg cttgccgaaa tataccagaa
    15241 gggaattctg gtggtaagaa aagtgaatat acagaactag ctttgatcaa aaatgagttg
    15301 ggtaaaaaaa ccagacatat tccaattagg caattggtta accgtgcatg taatgcgctg
    15361 gttgcaatta aaccttgttt catgatgggg ccaatgtcag cagctcatta cctagaacct
    15421 ggacgaatgg aatttgatct ggtggtgatg gacgaagcgt ctcaggtgaa gccagaggat
    15481 gcattgggtg tcatcgcgag gggcaagcaa ctagtggtcg ttggtgaccc gaaacagcta
    15541 ccaccaacca gtttctttga tcgaagtgcc gacggagaag atgacgatga tgccacggct
    15601 ttaagtgata ctgacagcat tttggatgct gctttgccac tgtttcctat gagacgtttg
    15661 cgttggcact atcgttcacg acatgaaaag ttgattgcat actctaaccg ccatttttat
    15721 aacagtgatt tggtgatatt cccttcccca aatgctgagt ctccagagta tgggattaaa
    15781 tttacctatg tgtcaaaagg tcggttctcc aatcaacaca atattgaaga agcccaagca
    15841 gttgctgagg ccgtacttca tcatgcgcaa caccggccgg gtgagtcact cggggtagtg
    15901 gccatgagtt ccaagcaacg cgatcaaatt gagcgcgcta tcgatgaatt gcgccgaaat
    15961 cgccctgaat ttaacgatgc aatcgatggc ttatatgcca tggaagagcc actttttgtg
    16021 aaaaaccttg agaacgttca aggggatgag cgtgatgtaa tctttatttc ctttacctat
    16081 ggaccttctg agcatggtgg aaaggtttat caacgctttg gacctatcaa ttccgatgtt
    16141 ggctggcgtc gcttgaatgt gcttttcact cgatcaaaaa aacggatgca tgtgtttagt
    16201 tcaatgcgtt ctgaagatgt attgacgagt gaaaccagta aacttggtgt tatttcgttg
    16261 aaaggttttt tacagtttgc cgaaagtggc aaactagatt ccctcacaac gcataccggc
    16321 agggctccag atagtgactt tgaggttgct gtaatggaag cactcaatca cgctgggttt
    16381 gagtgtgaac ctcaggtagg ggttgcagga ttctttattg atctagctgt gaaagatcca
    16441 ggttgtcctg gccgttactt aatgggcata gagtgtgatg gtgcggctta tcactcagct
    16501 aaatctgctc gtgatcgtga ccgtttgcgt caagaggttc tggagcgttt gggttggaga
    16561 attagccgca tttggtccac tgattggttc agtaatcctg atgaggttct atctccgatt
    16621 atccgtaaac tccatgagct taaaacattg gctccagacg ttgttgtacc ttcctatgaa
    16681 tatgtagaaa cgattgagtc aagcgctgaa gtggcgtctg actcaattga ttctcttatg
    16741 cccaatttgg ggcttaagga gcaacttaag tattttgcca cacatgtcat tgaggttgag
    16801 cttcctaatg ttgatgctga tcgtcgtttg ttgcggcccg caatgcttga ggctttgctg
    16861 gaacatcagc ctttatcacg ttccgagttt gttgaacgaa tacctcatta tctgcggcaa
    16921 gcaacagatg tatacgaagc gcaacgcttt cttgaccgag tcttggcatt aattgatggc
    16981 gcagaggctg aagcgaatga tgcagcgttt gagtctgaat tggcataatt agttaaaggt
    17041 aataagaaca gtgacaactg tcggacaacg tgaacgcgca actcaacagc gcgtagtcag
    17101 gttttttatt gaggaattgg gctatcgtta tctaggcgat tggcacacgc gtcctaacaa
    17161 ccgtaatgtt gagcctgatt tgctcagtca ttggttgatt gaccgtggtg ttgtggactg
    17221 accccacgcc cgtagacaaa ttctgtcctc acgacgaggc ctgttcgaag gcctccggac
    17281 tgacgccgcc gaggttactg tggcgccggg cccggttgta gaacacttca atgtaatcga
    17341 agatatccgc ccgggctaga tcccgggttt tgtatattct cttcctgatg cgctcttttt
    17401 tcagtgaact gaagaacgat tcggccaccg cattatccca gcagttgcca cgccggctca
    17461 tgctcgggac caggttatta gcccggcaga agcgctgcca gtcgtcactg ccgtactggc
    17521 ttccccggtc tgagtgcacg atgacctcgc cgtcgggttt acgtcgccag accgccatca
    17581 tcagtgcatc cagcgccagt tcgcgtgaga gagtgggctt catcgaccag cccaccacgt
    17641 tacgggcgaa gagatcgata accaccgcca gatacagcca gccctgccag gtgcggagta
    17701 gagtaagagg cagggcgtag tcggactatt tccctgcctc tcctccccga accggacgtg
    17761 cacctttcag cgcatccggc tctccattta aatgctggcg aacgccattg ccagtaagcg
    17821 taaactgacc gccgtatgta gccatcagac gagaattggt aacttagacg cccatctgat
    17881 atagacggac atctaagtat ggaattacag gactggagaa aagaacctcg taagcgttaa
    17941 cggagcgccg tattgacgct tatttattgg tgagtactac gttccatggc aggagttcat
    18001 caactcggtt ggaaggccat tctggcagca cgctcaggat atggcgcaga tacgcttccg
    18061 gatcgatacc gttcagacgg caggtgccga tcagcccgta caacagtgct ccacgctcgc
    18121 cgccgtgatc gctgccaaag aacataaagt ttttctttcc gagacagact gcacgaagcg
    18181 ctctttccgc agcattatta tccgcctccg ccagaccgtc atcactgtaa taacagaggg
    18241 catcccactg attcagtaca tagctgaacg cttcgcccag tctggatttt ttcgacagcg
    18301 taccattctt ctccaccatc cattcatgca gtgacgtcag taacactttg cttcgctgct
    18361 gcctgacggc aagacgctct gactccggta atccccgtat ttcatcctcg atggcgtaca
    18421 gttcactgat tcgcttcagg gcttcttctg ccgtcgcact tttgctgctg atgtatacat
    18481 cgtggatttt tcgccgggca tgggcccagc acgcaacttc tgtcagtgca ccaccttcac
    18541 gttctgcact gaacagccga tcgtaaccgc tgaacgcatc cgcctgcagg ataccccgga
    18601 agggacgaag gtgttgtacc ggatgttttc cctgcctgtc tggcgagtag gcgaaccaga
    18661 ccgctggagg agatgacgaa cccacattgc gatcatcccg gacatacgtc cagatacgcc
    18721 ctgttttcgc ctttttctga cccggtgcca gtacctttac cggtgtatca tcagtgtgaa
    18781 ccttgcgggt gttcatcacg taacggtaca gggtatcatt cagcagagtc attaactgac
    18841 aacatgcgtc aacccagttg gagagtaatg cccggctcag ttcgatgccc tggcgggcaa
    18901 agatttcact ctgacgatac agtggcaggt gttcgcagta ttttcccgtt aacacgcggg
    18961 caagtaatcc ggagcccgcg atgccgcgct ctatcgggcg ggacggcgct ggcgcttcaa
    19021 ctatacagtc acattttgta caggcttttt ttacccgaac agtgcggatc actttcaggg
    19081 cgctactcac cagttccagc tgctcagcac tgacttcccc cagataatcc agctcactgc
    19141 cacactccgg gcaacagctt tcttctggct ccaggcggtg tatttcacgg ggaaggtgtg
    19201 ccggtaacgg acgacgatgg cgcgactgtc gcaactggcg gggaacctga ggatcgtctt
    19261 cccgcccact gtaacgatcg ctgtcctgtt cacgttgttt cagcagagcc tcagccagtt
    19321 caacttcacg acgcagtttt tcagaacggg taccgaacag catccggcgc agtttttcta
    19381 tctgagcccg cagatgttct atttcccgtt catcttcttc gatcttttct tcggcacgtg
    19441 tcagtgcaga gcgcaggaag gcttccgtct cttcaaccag actcagttgc tggtctttct
    19501 gacggagggc ttcagcctgc tcagagagca acctttccag ctctgcgatg cgaatgaggt
    19561 atttctgact catgaccgtt tttataatgc ggtcaggagt tttttacaac attgtcagtg
    19621 agttacggct ggatgttttt ggctgacgcc agtccagctt atcgaggagc attgccagtt
    19681 gcgagcgggt aatggatacc ttgccgtcac gtaccgcagg ccagataaac tggccttcct
    19741 ccaggcgttt ggtgaacagg cacagaccat cagcatcagc ccaaagaatt ttgacggtgt
    19801 caccccgtcg gccacggaag ataaacaggt gaccggagaa gggattatca ttcagcacat
    19861 gttgtacctg ttctcccagt ccgttgaagg atttacgcat atcggtaacg ccggcaacga
    19921 gccagatacg ggtacctgat gggagtgaga tcatcttccc ctcccggtca gttcacggat
    19981 caacaccgtg agcagctctg gcgatggatt ttccagcgtc atgttaccgt gacggaattc
    20041 caccttgcag gaactggcac tgactctggt ctgagtggaa gtggataaag acggcgcaat
    20101 gaccgccaca ggttctttct gctcatccgg cgttatttct acaggtaata attcaacgcc
    20161 agtgtcagaa gaggtcgtta ccggaagacg ccgcgaaaca cgcccttcgt tctgccagag
    20221 cctgagccat ttgaaaataa cattatcatt gacgccattt tcacgtgcaa tctgtgcaac
    20281 acaagctcca ggttgtgatg ccagttccac catacgaagt ttgaattcat tcgaatagtt
    20341 tttacgaggt tcttttctcc agtcctgtaa ttccatactt agatgtccgt ctatatcaga
    20401 tgggcgtcta agttaccaat tctcgtctga tggctacata cggcggtcag tttacgctta
    20461 cacttaaaat ccttttatgc caagaaagcg aaacaagact ataccaaaac ctagaattcc
    20521 acctcccaca caccccatcc ttagacctct taaaacttcg gacaatctaa aatcaacttc
    20581 cccatacgct aagtatttaa tgataagcaa tatgaaataa gacaactcag caattgcaat
    20641 tcctgaaacc atggaggtaa ctatgacaaa gagactgaaa ataaatagtt tcattcacgc
    20701 actcctttct tcaatttatc attaatgact ttttgtagtt cagtccccgt catttctgat
    20761 gtaatagaag cccccatatt acctgcagcc cccggaatct tgctcggcac catttctttg
    20821 gagataccta acttcccttt aacttcagta tattttagta gagtcggatc gaatttagga
    20881 ttccagccac ctgttatcca cttgcctgct gtatttgcaa caggcttcac cacataattg
    20941 ccaaccccat ctccaaaccc ggcccccgct ctatttgtaa tcgacccagt aagaggatca
    21001 tctccgttta tagcgttggt taatgctcca cctgccgcat tccaagctat tgtccctttc
    21061 cagccctgcc ccatggttat cacatttatc cagcctgctg ccactgagtt gaccgggttt
    21121 atttcaccac tatctgcata ctgcatcccg acatttgtcc cggcaccaag taaccacata
    21181 gcctggacac tccccggtaa cagggcaacg ctgcctgttg ctaatgcggc tgcataagct
    21241 ttattttcat cctgcccccg cttacaggct tcaccggaag gattttcact acaggagaga
    21301 acatcagcac catgctcctt ggcttttgcc gtctggagcg attcactgcc acccagcaga
    21361 ttattctcaa caacaatccc aacccttttg gttgggattg ttgttgaact cattcagtat
    21421 ctgaaggcgg ttttttacgg gcgttaaatg catcaatcag tttaaaaacg acagcagcaa
    21481 ctgttatcgc tgtgcctgta attgttccaa aaacaaaagc tcttttgatt tgatgtaaag
    21541 ggaaaaacca atatcctttg ctgaaataaa ttaatgatga cactcctgcc cccagaacca
    21601 aacaaactcc gatagagaaa acgcaggcat agatgaatag tttcaggtaa ctaatcattt
    21661 tttgcccccg atttttcttc agatgggtta tttaatgctt ctttcgtgaa tccgttaata
    21721 aactcacgac caacacctcc gatagcatca taaacaaaat taggaatgtt attcgcattt
    21781 aatactttat ctacaccaac tggcgcatac ttcccaaacg ccccacccag accagcacca
    21841 ataccagccc cggctagtga agctgtattt ggaccatcag taaagaatgc cccacccatt
    21901 gcgatacctg tgtttgcaag gacaccacga ccgggagcca tcatgcctgt tgtaaatgag
    21961 gtcgcgcttc ccagatagtc ccacgactta ttcgcattct gcggctggtt caaatcatag
    22021 aactgataac cactattggc cgccagtcca agaagcccgc caccaagaac tttacctgct
    22081 gacgcagcag gccccagata ccatgccccg gcaatcatta caccatcctg atgtccatcc
    22141 acgatcactt tcgtgatatt gatgctgtcc gacatatcac ctttcgctat cttatccagc
    22201 ccagcctggg tttgttccgg tgtcagatta ttgcccaccg cgtacttgtt ccatgatgta
    22261 gccgcagcag cattgctttc aaaaccatca ccgaagctga gcgcgttatt ctcaacgaca
    22321 tagctatcga caaaaaacca tattaattct tttggaaaaa agaaaataag gtatccatat
    22381 aacaacacca aatattccac tcactaataa tctgatatta tctgaatcca gctgtacgtc
    22441 aaaaagaata gccggtaata aagtaagata gagagcaata atcagtcctg ttgcataata
    22501 agcgatcatg gcttttcgag ttcctttttt gcgtctgaaa aacaaccacg ttgcataaag
    22561 ggaaacaatg aatccacata tcagcaacgc aaatgccccc atagcgtaat aagataaaaa
    22621 gccagtttca ttgaaataat tgaatgcggt tatggtaaaa ctgtaaagtt caatagtact
    22681 accaataaca cccaagataa gtcccagagc aggaagatat aatagtccat taattttact
    22741 taactcctca ttttcacagg catcacacaa tcccgactct ttgtttgctt ctgacatttt
    22801 acattttata cattccattt tttattccat tattcattgc gttacgataa tgtttagttt
    22861 ttttaaacat cccccaccac atgtatggg
//
LOCUS       DAZFRN010000072        22358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFRN010000072 DAZFRN010000000
VERSION     DAZFRN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.072"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            35..2071
                     /gene="yihQ"
                     /locus_tag="ACHKUB_002702"
     CDS             35..2071
                     /gene="yihQ"
                     /locus_tag="ACHKUB_002702"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO6655690.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            2117..3502
                     /locus_tag="ACHKUB_002703"
     CDS             2117..3502
                     /locus_tag="ACHKUB_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6655691.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3545..4948
                     /locus_tag="ACHKUB_002704"
     CDS             3545..4948
                     /locus_tag="ACHKUB_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6655692.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5016..5708
                     /gene="ompL"
                     /locus_tag="ACHKUB_002705"
     CDS             5016..5708
                     /gene="ompL"
                     /locus_tag="ACHKUB_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO6655693.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5799..7064)
                     /locus_tag="ACHKUB_002706"
     CDS             complement(5799..7064)
                     /locus_tag="ACHKUB_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6655694.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7166..8146)
                     /locus_tag="ACHKUB_002707"
     CDS             complement(7166..8146)
                     /locus_tag="ACHKUB_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO6655695.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(8154..8864)
                     /locus_tag="ACHKUB_002708"
     CDS             complement(8154..8864)
                     /locus_tag="ACHKUB_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6655696.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(9081..10904)
                     /gene="typA"
                     /locus_tag="ACHKUB_002709"
     CDS             complement(9081..10904)
                     /gene="typA"
                     /locus_tag="ACHKUB_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO6655697.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            11277..12686
                     /gene="glnA"
                     /locus_tag="ACHKUB_002710"
     CDS             11277..12686
                     /gene="glnA"
                     /locus_tag="ACHKUB_002710"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO6655698.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            12860..13909
                     /gene="glnL"
                     /locus_tag="ACHKUB_002711"
     CDS             12860..13909
                     /gene="glnL"
                     /locus_tag="ACHKUB_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO6655699.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            13921..15330
                     /gene="glnG"
                     /locus_tag="ACHKUB_002712"
     CDS             13921..15330
                     /gene="glnG"
                     /locus_tag="ACHKUB_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO6655700.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            15442..15552
                     /locus_tag="ACHKUB_002713"
     CDS             15442..15552
                     /locus_tag="ACHKUB_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO6655701.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(15742..17115)
                     /gene="hemN"
                     /locus_tag="ACHKUB_002714"
     CDS             complement(15742..17115)
                     /gene="hemN"
                     /locus_tag="ACHKUB_002714"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO6655702.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(17304..17813)
                     /gene="yihI"
                     /locus_tag="ACHKUB_002715"
     CDS             complement(17304..17813)
                     /gene="yihI"
                     /locus_tag="ACHKUB_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO6655703.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(17932..18098)
                     /locus_tag="ACHKUB_002716"
                     /pseudo
     CDS             complement(17932..18098)
                     /locus_tag="ACHKUB_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18395..19027
                     /gene="yihA"
                     /locus_tag="ACHKUB_002717"
     CDS             18395..19027
                     /gene="yihA"
                     /locus_tag="ACHKUB_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO6655704.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(19409..22195)
                     /gene="polA"
                     /locus_tag="ACHKUB_002718"
     CDS             complement(19409..22195)
                     /gene="polA"
                     /locus_tag="ACHKUB_002718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO6655705.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         5380 a         5705 c         5505 g         5768 t
ORIGIN      
        1 aacaatctcg ttctcaactc tatggagatt agttatggat acgccacgtc cacagttatt
       61 agattttcaa tttcatcaga ataacgacag ttttacccta cattttcaac aacgtcttat
      121 tttaacccat agcaaagata atccttgttt atggattggc tcaggtatag cggatatcga
      181 tatgttccgc ggtaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga
      241 cgccatcgtc agccagtcac cggatggttg gttaattcat ttcagccgtg gttctgacat
      301 tagcgccacg ctgaatatct ctgccgacga tcagggccgt ttattgctgg aactacaaaa
      361 cgacaacctt aaccacaacc gtatctggct gcgccttgcc gctcaaccag aggaccatat
      421 ctacggctgc ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg
      481 gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg
      541 caaagaaaat gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag
      601 cacgcagaag tattactgcc atgttgataa cagttgctat atgaacttcg actttagtgc
      661 cccggaatac catgaactgg cgctgtggga agacaaagca acgctgcgtt ttgaatgtgc
      721 tgacacatac atttccctgc tggaaaaatt aaccgccctg ctgggacgcc agccagaact
      781 gcccgactgg atttatgacg gagtaacgct cggcattcag ggcgggacgg aagtgtgcca
      841 gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtc aacggcatct gggcgcagga
      901 ctggtccggt attcgtatga cctcttttgg caaacgcgtg atgtggaact ggaagtggaa
      961 cagcgaaaac tacccgcaac tggattcacg cattaagcag tggaatcagg agggcgtgca
     1021 gttcctggcc tatatcaacc cgtatgttgc cagcgataaa gatctctgcg aagaagcggc
     1081 acaacacggc tatctggcaa aagatgcctc tggcggtgac tatctggtgg agtttggcga
     1141 gttttacggc ggcgttgtcg atctcactaa tccagaagcc tacgcctggt tcaaggaagt
     1201 gatcaaaaag aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta
     1261 tctgcccacc gacacttacc tgcataacgg catcagcgcc gaaattatgc ataacgcctg
     1321 gcccgcgctg tgggcgaaat gtaactacga agcccttgaa gaaacgggca agctcggcga
     1381 gatccttttc tttatgcgcg ccggttctac cggtagccag aaatactcca ccatgatgtg
     1441 ggcgggcgac cagaacgtcg actggagtct cgacgatggc ctggcgtcgg ttgtcccggc
     1501 ggcgctgtcg ctggcaatga ccggacatgg cctgcaccac agcgacattg gcggttacac
     1561 caccctgttt gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc
     1621 cttcacgccg atgatgcgca cccacgaagg taaccgtcct ggcgacaact ggcagtttga
     1681 cggcgacgca gaaactatcg cccattttgc ccgtatgacc tccgtcttca ccaccctgaa
     1741 accttacctg aaagaggctg tcgcgctgaa tgcgaagtcc ggcctgccgg ttatgcgccc
     1801 gctgttcctg cattacgaag acgatgcgca cacttacacc ctgaaatatc agtacctgtt
     1861 aggtcgcgac attctggtcg ctccggtgca tgaagaaggc cgtagcgact ggacgctcta
     1921 tctgccggag gataactggg tccacgcctg gacgggtgaa gcgttccggg gcggggaagt
     1981 taccgttaat gcgcccatcg gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc
     2041 ggcactgttc gcgtcgttaa aaagcatcta atcacatccg cccgtgggga aactcatggg
     2101 caaagggaga acatcgatga gtcacatcac aacggaagat ccagcaactt tacgcctgcc
     2161 ctttaaagag aaactctctt acggtattgg cgacctggcc tctaacatcc tgctggatat
     2221 cggtacgctt tatcttttga agttttatac cgacgttctg gggctgccag gcacctatgg
     2281 cggcattatc tttttgattt caaaattctt tactgcgttt accgatatgg gtaccggcat
     2341 tatgttggat tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta
     2401 tgcgtcattc ccggtcacct tactggcgat cgccaacttt gtcggcacac cgtttgatgt
     2461 caccggtaaa acggtgatgg ccactattct gtttatgctt tacggactgt ttttcagcat
     2521 gatgaactgc tcctacggcg caatggttcc cgctatcacc aaaaacccca acgaacgcgc
     2581 ctcactggcg gcatggcgtc agggcggcgc tacgctgggc ctgctgctgt gcacggtggg
     2641 attcgtgcca gtgatgaatc ttatcgaggg taatcagcaa cttggctata tcttcgccgc
     2701 cacgctgttt tcactgttcg gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga
     2761 gcgttatgtc gaaacccaac caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt
     2821 ccgcgcaatt gccggtaacc gcccactgtt cattctgtgt attgccaacc tctgcacctt
     2881 aggggcgttt aacgtcaagc tcgccattca ggtctattac acccagtacg tacttaacga
     2941 tcccatcctg ttgtcgtata tgggattttt cagcatgggc tgtattttca tcggcgtgtt
     3001 cctgatgcct ggcgcagtca gacgttttgg taagaagaag gtctatatcg gcggcctgct
     3061 gatttgggtg ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt
     3121 ggcgttctcc tgcctggcgt tcttcggctc agcgtttgtt aacagcctga actgggcgct
     3181 ggtttccgac accgtcgagt acggcgagtg gcgcaccggc gtgcgttcgg aaggcacggt
     3241 ctacaccggc ttcaccttct ttcgcaaagt gtctcaggcg ctggctggtt tcttccccgg
     3301 ctggatgctg acgcaaattg gctatgtgcc gaacgtcgca caggctgacc acaccattga
     3361 agggttacgc cagttgatct tcatctaccc aagcgcactg gcggtagtca ccattgtggc
     3421 gatgggttgc ttctacagcc tgaacgagaa gatgtatgtc cgcattgtgg aagagataga
     3481 agcccgtaaa cgcacggcgt aataataata aacgacgccc tgcggggcgt tataaggagt
     3541 gattatgtct gaccataatc cactgacatt aaaactgaat ctgcgggaaa aaatcgccta
     3601 tggtatgggc gacgtcggtt cgaatttaat gctctgcatc ggtactctgt atctcctcaa
     3661 attttatacc gatgaattag ggatgcctgc ttactatggc gggattatct ttctggtggc
     3721 gaagtttttt accgcgttta ccgatatgct caccggattt ttactcgact cgcggaaaaa
     3781 tattgggcct aaaggcaaat ttcgtccctt tattttatat gcagcagttc cggcggcgtt
     3841 aattgcaacg ctccagttta tcgccaccac cttttgtttg ccggttaaaa cgacgattgc
     3901 caccgcgctg tttatgatgt ttggactttc atacagtctg atgaactgct cgtatggtgc
     3961 aatgatcccg gcaattacca aaaacccgaa tgagcgcgcg cagctcgcgg cttaccgcca
     4021 gggtggtgca accatagggc tattgatttg taccgtggcg tttattccgt tgcagtcgct
     4081 tttttctgac tcaaccgtcg gttatgcctg tgcggcactt atgttctcca ttggcggctt
     4141 tatttttatg atgctgtgct acagaggcgt caaagagcat tatgtggaca cagcgccaac
     4201 cggacataaa gccagtattc tcaaatcttt ttgcgcgata tttcgtaatc caccattgct
     4261 ggttttatgc attgctaacc tgtgtactct ggcggcattt aatatcaaac tggcgattca
     4321 ggtctattac acccagtatg tgctgaatga tattaattta ttgtcgtgga tgggattctt
     4381 cagtatggga tgcatcctcg tcggcgtatt actggtgcca gtaactgtaa aatgttttgg
     4441 taaaaaacag gtttatttag ctggcatggt gctatgggcg gtgggtgata tactgaatta
     4501 tttctgggga agtaactctt tcactttcgt catgttctct tgtgtcgcct tttttggcac
     4561 ggcctttgta aacagcctga actgggcact ggtaccagat accgtagatt acggtgaatg
     4621 gaaaaccggt attcgtgccg aaggttctgt ttataccggt tataccttct ttcgtaaaat
     4681 ttctgccgca cttgctggct tcttgccagg cattatgctg acgcaaattg gttatgttcc
     4741 caacatcgcg caaagcgatg cgacattaca gggtttgcgt cagctcattt ttatctggcc
     4801 ttgtgcactg gcaattattg ctgcattgac gatgggattc ttttacacac tcaatgaaaa
     4861 acggtttgca ttgattattg aggaaatcaa ccaacgcaaa aataaagaga tggcgacaga
     4921 agaaaaaacg gcttccgtaa cgttataaat gtcaatagcc gctatttcca tcctggtggg
     4981 tggcggcctc cctacgttta aaaaatggac ttattatgaa aaagattaat gcaataattt
     5041 tattatcttc tctgacctct gcctcggtat ttgctggcgc atacgtcgaa aacagggagg
     5101 catataatct tgcctccgat cagggcgagg ttatgctacg tgtgggttat aacttcgata
     5161 tgggcgcggg tattatgtta accaatacct acacctttca gcgagaagat gaactaaaac
     5221 atggctataa tgaaattgaa ggttggtatc ctttattcaa accaaccgat aaattaacca
     5281 tccagcccgg tggcttaatt aatgataaga gtatcggttc gggtggtgca gtgtatctgg
     5341 acgtcaacta taaatttgta ccatggttta atctgacagt acgaaatcgc tataaccata
     5401 ataactatag ttcaacagat ttgagcgggg aactggataa taatgacacc tatgaaattg
     5461 gcacctactg gaattttaaa atcaccgata aattttccta tacatttgag ccacattatt
     5521 tcatacgcgt aaatgacttt aatagcagca atggaaaaga tcatcactgg gaaatcacta
     5581 ataccttccg ttatcgcatt aatgaacact ggctgcctta tttcgaatta cgttggttag
     5641 atcgtaatgt cgaaccgtac caccgcgagc aaaaccagat tcgtatcggg acgaagtatt
     5701 tcttctgatg tgaaactgcc ggatgcggcg taaacgcctt atccggccta cggttgatac
     5761 ggttttcgtt ggccggatta agcacatccg gccattaatc aagcttcagc cgccagcatc
     5821 tctttcttac gttggctaat tcgcttaacc agcattacgg aaaccaccgc accacagaat
     5881 gcgaagcagg ccataatgcc aaacacgatt ttgtagccaa taatccccgg atttaaatcc
     5941 agaatgtagc catacagact gaagcagaac atcgccggag cgtaaccaat aaagctaccc
     6001 aacgccatcg ccgcgcctgt tttattttca gcaatttttg cttcgccgat aggtgcaaaa
     6061 aatacggctc gctgtgtaaa gactatcgcg ccaaagccca gcgtacatgc catccctaaa
     6121 tagaccggca tactttcgtg cggcagcata atcaacagta cgagcgcagc ggtactgatg
     6181 ataaaggtgt agcatagata tttactcggc gatttcagga tcttatctga aatcatgcca
     6241 ccaatcggtc caccaatcat tttcagacag tattggttaa tgatgccgta agcccccacc
     6301 agcgcaacgg gcaatagata gatgtttttc aggaatggaa tgaagaatgt caggccacag
     6361 taaaccgcgt aaacgaagaa gacgttaaaa gcgataagcc agatggtttt gtctctcagc
     6421 accgaggtca tactggtgtt ttgcgatgct ccgtcttctt ttttcacctc aacggacggt
     6481 gcctcttctt tgtcattcag gacaaagaaa ataataatgc ctacggcaat cacaataagg
     6541 gaatagaacc agatgcctgc tttaaaaccc aataagccac tgccaaacca ggtaaatact
     6601 gccaacgcag aaaatgccac cacggtatcg acaatgccac gccctgtttc gaagaagcca
     6661 aacaaccgac cttgttgttc gctattgccc aatcgactca ccgacttgag caagaccggc
     6721 cagttcatca tgtcgcaagt aacgccaaat agcgcccaga caaagaggat gccccaatac
     6781 cccggcattg tcgtcaaata aacacccagt aatcctgtcg caatgagtga gaatgacatg
     6841 gtgtatctgc gcggtagttt atcggcaaaa taaatagaca gaaaaaaacc cactgtcgtg
     6901 acaaatgagt ttaccgacat agcattacca atttgaccat tggttaaatg gaaatattcc
     6961 tgcatcggga tataaaacgc atctttcagc gacggtaatt tataaattgt accgccacac
     7021 aatgttaata gactaaataa cgcccatttc ttttttgtga gcataacatc ccctaagcga
     7081 tttttaaaat cactgtttta aaggcaataa gataccgacc attcatatgg aatggtttta
     7141 ttaactgcgt atgagttaaa acttattagt ggttcagaag atggtttgcc tcttgtttga
     7201 tttgttgtag tacgttacgc acatgattta tctgattaat tgcatcttct ttttctttac
     7261 gtaaccgcgc atccagtaaa tgattttctg gcaatggtgt ttcactcatc tggcgataag
     7321 ggatccacgg attatcatct tcgtcttcaa agcggaacgc cggcgcatag taatcaacct
     7381 cttcttccag gccatatgcc gtcacctgcg gctcatcatc acccaggcat ataaggtgcg
     7441 ttaataacgc tttgaacggc atatcaccct gacctgaaat acaggcttta tgacccaggc
     7501 cacccggttc tttaacgatc aaggcatctt tgatatggac ctgggtaata tgcggcgcca
     7561 tcgtttttaa agcgtcgatg ggatgctcat ttgcattgat catgttcgca aaatcaaaca
     7621 gtaaggaaag agattccatc tcactttctt tgaccagcga caccagctca tggcttttta
     7681 aatcttcatg ctgttcgatc gtaaaagtca ggccgctgtc ctgatacgtt tcccgtacat
     7741 aggcaatgtc gttagcgatg atcgataata cgtcgcgcaa attaccttca taacgtggat
     7801 aaaaacgtac ggacgatgcc ccagttttca acgcaatggc gacggcttcg tcaatagatg
     7861 ccttatctga agcgctggtt tcaatatgaa tatcaaggtt cagacggcgg gctttatccc
     7921 caaaggcaga gagttcttta tcgtccatat taccaagaga aaaacgctcg ccatccagaa
     7981 catggatttt gacgccacgt agattatttt cacttgcgat atcaagtaaa tcagcaggca
     8041 acactctttc cagccgcata tttaaatgaa aggcataagc atgtaaataa agaggaagag
     8101 tgtcgacacg ttgtagaatc tttcttgcgt tatttattgt caccataaaa cacctacaga
     8161 ttaatgatga ggtctacggc ctcaaatcgc caatattcaa tatctatctg gacaatattg
     8221 ccttgctggt cggcgcgatg cttttcaatt aagatcgctg gcatacccgc agaaccgcca
     8281 agatacttgc tgatatcgcc cggcattctg acgggtttga aaatcaattt ttttactacc
     8341 gtctcttttt ggtagcactt ttcccagact gcagaaaatg agtggttttc aagttgttca
     8401 ataaaacccg gagcaacttc aggattaata tatgtttcgt gatagaaaac tttatgtcct
     8461 tccagcgccc cccagccggt gatgcgatag agttcagttg atggtgtcac tgcaatcagt
     8521 ggcgcgagcg tctcgggaat atcgctggta cggtttttct cggtaaaccc ccaggaaggc
     8581 tctcgtcctt gctcaattgc ggcccgctga aagctcgccg acaactccgg actgtaatta
     8641 aatcgcggct gggtcacgaa ccacccctta cgatccttac ggaagatttt ggattctgcc
     8701 tcgaggttca acaacgcctg gcgcagcgtc atacgtttaa tgcccagcag ttctcccagc
     8761 tctctttctg aagggagttt tccacccggc gtggtgatgc catctttcaa ccaccgatcc
     8821 agtttctctt ttgcattttc tacggtggat tgattctccg ccattgttta atccttttgg
     8881 tttaaaccag atgaggcatc ttacgcttac gccaaaaaag aaagcaatta acggcgtatg
     8941 actttgcaga taattgtgac aaagctctct attgacgtaa tcccagactt ataagggaga
     9001 tactcgcatt ggtgtgcgct cacgacaggt aaaaaaaaac ctgccagcga tggcaggttt
     9061 tttataacta aaaaacgaaa ttaatcgtct ttcggtgcgc ggttggcgcg acgacgatcg
     9121 ttttccgtca ggtgacgttt acgaatacgg atagaggtcg gggtcacttc taccagttcg
     9181 tcatcatcga tgaactccag agcttgttcc agagtcatgc ggataggcgg aaccagaaca
     9241 acggcttcgt cagtaccgga agcacgcatg ttggtcagtt tcttaccggt caggcagttt
     9301 acagtcaggt cgttagagcg gctatgaata ccgataatct gaccttcgta aacttctgca
     9361 ccgtgaccga ggaacagctt accgcgatcc tgcagaccga acagcgcgaa cgcgaccgct
     9421 ttaccctgac cgttagagat cagtacgccg ttctgacgct gacccacttc acccggacgt
     9481 acgtcgtcgt agtggctgaa ggtggagtac agcagaccag taccggaagt catggtcatg
     9541 aactcagaac ggaagccaat cagaccacgg cttgggatca cgtagtcgag acgtacgcgg
     9601 cctttaccgt ctggattcat gtttttcagg tcgcctttac gttcgcccag cgcctgcatt
     9661 acagaaccct gatgctgttc ttcaacgtcc agagtcacgt tttcatatgg ctcttgttta
     9721 cgaccgtcga tttcacggaa gataactttc ggacgggata ccgccagttc gaaaccttca
     9781 cgacgcatgt tttcgatcag aacagacagg tgcagttcgc cacgaccaga aacgcggaac
     9841 gcatcggcgt cttcggtttc ttctacgcgc agcgcaacgt tgtgtaccag ttctttgttc
     9901 agacgatcca ggatctgacg agacgttacg aacttacctt ctttaccgca gaacggcgag
     9961 gtgttaacgc agaagaacat agaaacggtc ggctcatcaa cggagagtgc cggcagcgct
    10021 tcaacgtttt gcgtgtcgca aacggtgtca gaaatgttca gttcgccaag gcccgtgatc
    10081 gcaacgatat cgccagcttc cgccagatcg gtttcgatac gttccagacc gaggtggccc
    10141 agcactttac cgactttcgc gttgcgggtt ttgccttcgc tatcgatgat agtgacctgc
    10201 tggttcggct tcactttacc gcgcttgatg cggccaatgc cgataacgcc aacatagctg
    10261 ttgtaatcga gctgagaaat ctgcatctgg aacggaccgt caaggtcaac gtccggcgca
    10321 ggaacgtggt caacaatcgc ctggtacagc ggggtcatgt cttccgccat atcttcgtgg
    10381 tccagacccg cgataccgtt cagcgcagaa gcgtaaacga tcgggaagtc cagctgctcg
    10441 tcggtcgcgt cgaggttaac gaacagatcg aatacctgat ccacaaccca atcaggacgc
    10501 gcgccagggc ggtcaacttt gttgataaca acaatcggct tcaggccgta agcaaacgct
    10561 tttttggtta cgaagcgcgt ttgcggcatc gggccgtcaa atgcgtcaac caccagcagc
    10621 actgagtcta ccatggacat tacacgttca acttcaccac cgaagtcggc gtgccccggg
    10681 gtatcaacga tgttgatacg gtaatcattc catttgatag cggtgttttt cgcgaggatg
    10741 gtaatcccac gctctttctc caaatcgttg gagtccatca cgcgctcttg ggtttcggca
    10801 cgagagtcga acgtaccgga ttgttggagc agcttgtcta ccagggtggt tttaccatgg
    10861 tctacgtgcg cgatgatggc gatattacgc aatttttcga tcacaacttt gcctcaggca
    10921 ttagaaatag cgcgttattg tacacggatt aatcgcacta caaaacagga tcacaaacat
    10981 cctccgcaaa caagtattgc agagtccctt tgtgatcgct ttcacggagc ataaaaaggg
    11041 ttatccaaag gtcattgcac caacatggtg cttaatgttt ccattgaagc actatattgg
    11101 tgcaacattc acatcgtggt gcagcccttt tgcacgatgg tgcgcatgat aacgcctttt
    11161 aggggcaatt taaaagttgg cacagatttc gctttatctt ttttacggcg acacggccaa
    11221 aataattgca gatttcgtta ccacgacgac catgaccaat ccaggagagt taaagtatgt
    11281 ccgctgaaca cgtactgacg atgctgaacg agcacgaagt gaagtttgtt gatttgcgct
    11341 tcaccgatac taaaggtaaa gaacagcacg tcactatccc tgctcatcag gtgaatgctg
    11401 aattcttcga agaaggcaaa atgtttgacg gctcctcgat tggcggctgg aaaggcatta
    11461 acgagtccga catggtgctg atgccagacg catccaccgc agtgattgac ccgttcttcg
    11521 ccgactccac cctgattatc cgttgcgaca tccttgaacc tggcaccctg caaggctatg
    11581 accgtgaccc gcgctctatt gcgaagcgcg ccgaagatta cctgcgttcc actggcattg
    11641 ccgacaccgt actgttcggg ccagaacctg aattcttcct gttcgatgac atccgtttcg
    11701 gatcatctat ctctggttcc cacgttgcta tcgacgatat cgaaggcgca tggaactcct
    11761 ccacccaata cgaaggtggt aacaaaggtc accgtccggc agtgaaaggc ggttacttcc
    11821 cggttccgcc ggtagactct gctcaggata ttcgttctga aatgtgtctg gtgatggaac
    11881 agatgggtct ggtggttgaa gcccatcacc acgaagtagc gactgctggt cagaacgaag
    11941 tggctacccg cttcaatacc atgaccaaaa aagctgacga aattcagatc tacaaatatg
    12001 ttgtgcacaa cgtagcgcac cgcttcggta aaaccgcgac ctttatgcca aaaccgatgt
    12061 tcggtgataa cggctccggt atgcactgcc acatgtctct gtcgaaaaac ggcgttaacc
    12121 tgttcgcagg cgacaaatac gcaggtctgt ctgagcaggc gctgtactac attggcggcg
    12181 taatcaaaca cgctaaagcg attaacgccc tggcaaaccc gaccaccaac tcttataagc
    12241 gtctggtccc gggctatgaa gcaccggtaa tgctggctta ctctgcgcgt aaccgttctg
    12301 cgtctatccg tattccggtg gtttcttctc cgaaagcacg tcgtatcgaa gtacgtttcc
    12361 cggatccggc agctaacccg tacctgtgct ttgctgccct gctgatggcc ggtcttgatg
    12421 gtatcaagaa caagatccat ccgggcgaag ccatggacaa aaacctgtat gacctgccgc
    12481 cagaagaagc gaaagagatc ccacaggttg caggctctct ggaagaagca ctgaacgaac
    12541 tggatctgga ccgcgagttc ctgaaagccg gtggcgtgtt cactgacgaa gcaattgatg
    12601 cgtacatcgc tctgcgtcgc gaagaagatg accgcgtgcg tatgactccg catccggtag
    12661 agtttgagct gtactacagc gtctaatagt tgaagttgta ctacccggcg caacaacgcc
    12721 gggatttagt tgccgtggaa actttcagcc catctctgca tgggcttttt tctccgtcaa
    12781 ttctctgatg cttcgcgctt tttatccgta aaaagctata atgcactaaa atggtgcaac
    12841 ctgttcagga gactgcttta tggcaacagg cacgcagccc gatgctgggc agatcctcaa
    12901 ctcgctgatt aacagtattt tgttaatcga tgacaacctg gcgatccatt acgccaaccc
    12961 cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg tttggtacac cgttaccgga
    13021 actgttgagc tacttctcat taaatatcga gctgatgcaa gaaagtctgg aggcggggca
    13081 aggttttacc gataacgaag tgacgctggt catcgacggg cgctcgcata tcctttctgt
    13141 gacggcccag cgtatgccgg acggcatgat cctgctggag atggctccga tggataacca
    13201 gcgccgctta agtcaggaac agctacagca cgcccagcag gttgctgccc gtgatttagt
    13261 gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg cggcgcagct
    13321 actcagcaaa gcgttacctg acccatcact actcgaatat accaaagtga ttatcgaaca
    13381 ggcggaccgg ctgcgaaatc tggtcgaccg tctgttgggg ccgcagctgc ccggtacgcg
    13441 cgttaccgaa agtattcaca aagtagctga acgcgtggta acgctggtgt cgatggaact
    13501 gccggacaac gtgcggttga ttcgtgatta cgatcccagc ctgccggaac tggcgcacga
    13561 cccggatcaa attgaacagg tcttgctgaa tattgtgcgc aatgcgctac aggcgctggg
    13621 gccggagggt ggtgaaatca ttctgcgtac ccgcaccgcg tttcaactga ccttacacgg
    13681 cgagcgctac cggctggcgg cgcggattga tgtggaagat aacgggccgg gcattccgcc
    13741 tcatttgcag gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg gcaccgggct
    13801 tggcttatcc atcgctcgta atttgattga tcagcattca ggcaaaattg aatttaccag
    13861 ttggccaggg cataccgagt tctcggttta cctgcctatc aggaaataaa ggtgacgttt
    13921 atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg ggtgcttgaa
    13981 cgtgcgctcg ctggggcagg tttaacctgt acgacgtttg agaacggcgc agaagtgctg
    14041 gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag atatccgtat gccgggaatg
    14101 gacgggctgg cgctgctcaa gcagattaaa cagcgccatc caatgcttcc ggtcatcatt
    14161 atgaccgcac attccgatct ggatgctgcc gtcagcgcct atcaacaagg ggcgtttgat
    14221 tatctgccca aaccgtttga tatcgacgaa gcagtggcgc tggttgagcg cgctatcagt
    14281 cattaccagg aacagcagca gccgcgtaat gttcagctta acggcccaac gaccgatatc
    14341 atcggcgaag cgccagccat gcaggacgtg ttccgtatta tcggtcggct ttcgcgttct
    14401 tctattagcg tgctgattaa cggcgaatcc ggcaccggta aagaactggt cgctcatgcc
    14461 ctgcatcgcc acagtccgcg cgccaaagcg ccgtttatcg cgctgaatat ggcagctatc
    14521 ccaaaagatt tgatcgaatc agaactgttt ggccacgaga aaggcgcgtt tactggcgcg
    14581 aataccattc gtcaggggcg ttttgaacag gccgatggcg gtacattatt cctcgacgaa
    14641 attggtgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc agacggtcag
    14701 ttttaccgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat cgctgccact
    14761 caccagaatc tcgaacagcg agtgcaggaa ggtaagttcc gtgaggatct gttccaccgc
    14821 ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac gtcgggaaga tattccccgt
    14881 ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg gcgtagaagc gaagttgctg
    14941 catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc caggcaacgt acgccagctg
    15001 gaaaacacct gccgctggct aacggtgatg gccgccgggc aggaagtgtt gattcaggat
    15061 ttgcccggcg aactgtttga atcaacggtt gcggagagta cttcgcaaat gcaaccggac
    15121 agttgggcga cacttttagc gcagtgggca gacagagcgc tgcgttccgg tcatcaaaat
    15181 ctgctttccg aagcgcagcc agagctggag cggacgttac tgacgaccgc gttgcgacat
    15241 acgcaggggc ataaacagga agcggcgcgg ctactcggct ggggccgcaa caccctgacg
    15301 cgtaagttaa aagagctggg gatggagtga ttcacagctt gtgtgtaaag attgattatt
    15361 gagcgcaaat tgctggtatt ttacgcttta ctgttccgat aagttcagta tgatcttgcc
    15421 cggaaaacgg ggagagtcat tatgctggaa tcaataatta atctggtatc gagtggcgca
    15481 gttgacagcc acacaccgca aactgccgtt gctgccgtgc tgtgtgccgc aatgattggg
    15541 ctgtttagtt gagtttcaga ttgctgatga agtgctcttt gttcatgccg gatgcggcgt
    15601 gaacgcctta tccggcctac aaaagcaata ttttcaacag gttttgtagg cctgataagc
    15661 gtagcgcatc aggcaatctt gttctcagca cattgatggc gctttcgttt tctactttgt
    15721 aaacgaagcg ccattcacta tttaaatcac ccgtgagaac tgctgcatcc gcgctttctg
    15781 gcgcagatag gtatcaaagc acatgcaaat gttgcggatc agcaagcgac ctttcgccgt
    15841 cacctgtatt cccttctcat ccacatccac cagcccatct tttgctaacg gggcgagcag
    15901 cttgagatct tccgcaaagt aatcagcgaa gtgcaaatcc cactgtttct caataggggc
    15961 gtaatccaga cggaagttgc agatgagcga cttaatcaca tcgcggcgaa tacagtcatc
    16021 acgcgttagc gcaataccac gccacagcgc attgccttgt tcatccactt gctgatagta
    16081 ctgcttcaac tctttctggt tctgcgcgta gcagtcgcca atcatgctga tggcggaaac
    16141 gcccatcccc agcagatcgg tatcgccctg agtggtgtag ccctggaagt tacgatgcag
    16201 cacgccttca cgctgggcca ccgccagctc gtcatccgga cgggcaaagt gatccatacc
    16261 gataaactga tagcccgatt gcgtcaggaa ggcgatggtt tcctgcagga tatcgagttt
    16321 ttgctgcgga ctcggcaggt cagcatcttt gattttgcgc tgagcagcaa aaatggtcgg
    16381 cagatgcgcg tagttaaaga cactcagacg atcggggttc agctccgcca cacgtttcag
    16441 ggtaaaggcg aaactctccg gcgtctgttt cggcaggccg taaatcaggt cgatgttggt
    16501 ggaggtaaaa ccaatctcac gcgcatggtt aagcagtgca aagatgaact cttcatcctg
    16561 ctcgcggtta accagacgtt gcacttcttt gttgaagtcc tgcacgccca tgctcaggcg
    16621 attaaagcct tcggcgcgta aatgatcgag tacatccagt tcgatttccc gcggatcgac
    16681 ttcgatcgaa atctccgcat cggcattgaa ctggaagttt tcgcgcagca gcttcatcag
    16741 gcggctgatt tgcgctttat tcagatacgt cggcgttccg ccgccccagt gcaattggct
    16801 gacgtgacgc ccggcgaaca gcggtgcacg atggacgatt tcttgctcca gcgcgtccag
    16861 atactgatcg gccttgtgct gctggcgagt aacaatctta ttgcaaccgc agaagtaaca
    16921 aagcttatgg cagaacggga tatgtacgta gagagataat ggacgctcag gatagcgcgc
    16981 cacggcttgt aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg cggtcgggta
    17041 cgaggtgtat cgtggcccgg aatagttata tttctggatc agggccagat cccagtcgat
    17101 ttgctgtaca gacatgctca ctccttccga tggcgtctct ggcggctacg gcgtaccggc
    17161 ggatgcccgt ttcgggtcat cagccggttg cgcagccact tttgtcgccg cgacaaccgc
    17221 tgtagtttaa cgaataacca caccagataa catataaccg gaagggttat cagcagaacg
    17281 ggaaggccca cggcggcaat ccgttagttg ccccgcagca gacgcatcat gtcttcttgc
    17341 ttctcgtctt cttcctcttc ttcgtcatcg tcataagaga ggccgagttt ctgcatcaac
    17401 tcatcaatac gatccagttt agcatccacc caggattgct cttcggcgct cagggtttcg
    17461 cctgcttcca gacgttccag cagcgcatcc agacgctcat ccgtttccag taattccaac
    17521 tccgcctgcg gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt ggtgactttt
    17581 tcagtcacgc ccaatggaat aggggtttta ctgccaatac gtggatcttt tggtgcgttc
    17641 tggcctttgc tgcctgacgt ggtgtttccg cccgctgcgc ggctgcccgg cgcatgacca
    17701 cgacgttttt tctgacgctt gcgatcacga gcttcctgat ccagctcctc gcgtgttttt
    17761 cgacgcgctt ttgcatgacc tttgctgcgt gagtttgaag atgatggttt cataatggtt
    17821 cgtcttttag ccgttttatt cagtatagat ttgcggcgga atctaacaga aagcaagcaa
    17881 agaaaaaagg cgacagatta ctctgtcgcc ttttttcctg actcataacc cttaacgggt
    17941 cttacaatcc ttgcaggccg ccaacacacc ctgtttttcc tttagccgga acaatccgtg
    18001 ttgattccat ttccgtttaa ttacgtctcc ggacgtttgt cttcctgaca atcctctgtc
    18061 ttcgcctcct ggcgctcctg accctcatcc tgagtcattg ttcctgttag cgtcctcgct
    18121 ctatgggtgc tactttacgc ctttgcttta aacaaacaat caaccgccat cagtatttgc
    18181 acattttcag agttttatat aaaagcaatt cactgattta tatagcttat ttgacatgca
    18241 tcgcagaatt ttctctgcta tttcgcataa agactatcgg caatgtctca caaagcgtat
    18301 ggcttttact tacaacgctc acgccgaaaa atccgccttt tattatggtt caggtataat
    18361 cgccacaaag ctttgactga cggagacgac cgctttgact aatttgaatt atcaacagac
    18421 gcattttgtg atgagtgcgc ctgatattcg ccacctacct tccgataccg gaattgaagt
    18481 ggcttttgca ggccgttcca acgcaggtaa atccagcgcg ctgaacacgc tgactaacca
    18541 gaaaagcctg gctcgtacct caaaaacccc agggcgcacc cagcttatca acctgtttga
    18601 agtggctgac ggcaagcgtc tggttgactt gcctgggtac ggttatgcgg aagtcccgga
    18661 agagatgaag cgcaaatggc agcgtgcgct cggcgaatac ctcgaaaaac gtcagagcct
    18721 gcaaggtctg gtggtgctaa tggatattcg ccatccgctg aaagatttgg atcagcagat
    18781 gattgagtgg gcggtagaca gcaatatcgc cgttctggtg ctgctgacca aagcggacaa
    18841 actggcaagc ggcgcacgta aagcgcaatt gaatatggtg cgtgaagctg tactggcgtt
    18901 taacggtgat gtgcaggttg aaacgttttc ttcgttgaag aaacaaggcg tggacaagct
    18961 gcggcagaaa ctggatacct ggtttagcga gatgcagcct gtagaagaaa cgcaggacgg
    19021 cgaataattt tcttgcctta atgcttgtgc cggatgtggc gtatccggcc cgtaaattca
    19081 atagattaca attctaccgt tggcctgata aacatagcgc atcaggcatt acggatcttt
    19141 tctttcgccc aataaaaaac gccccagtca ttactgactg gggcggctaa aatattcagc
    19201 caaatccgat tacgtgaagt aaaaggtctg aaagatagaa catcttacct ctgtacccta
    19261 cgcgactaac tttactcttt tttgttcagt tcagaaagca ctttttgtaa ttttttttca
    19321 gttgttgcat aggaatttct aatagccatc acaaaacgtg acagcttatg ttgcttactt
    19381 acgaaaaaag ggcatgttca ggcgaatctt agtgcgcctg atcccagttt tcgccactcc
    19441 ccacttccac cagcaacggc acatccagac gggtacagtt ttccatcagt tgatgaatct
    19501 gcttcgcgac ggcatcaaca tcatctttat gaacttcaaa taccagttca tcgtgtacct
    19561 gcatgatcat acgtacacgc ggttgctcag cctgtaacca cgcatcaacg gcaatcatcg
    19621 cccgtttgat aatgtcggcg gcggttccct gcattggcgc gttaatggct gcacgttcag
    19681 ccgctgcacg acgagcacca ttgctggatt tgatatccgg cagatacaga cggcgtccgt
    19741 ccagcgtttc aacgtagccc tgctctttcg cctgagcacg ggtgcgttcc atatactcca
    19801 gcacgccagg gtagcgttcg aagtaaaggt ccatgtactt ctgcgcttct ttacgtggaa
    19861 tgttcaattg ccgcgccaga ccgaaagcac tcatgccata aatcagacca aagttgatcg
    19921 ctttcgcgct acggcgttgc tcgctggtga cggtttccag tggcaaacca aacacttctg
    19981 ccgccgttgc ccggtggata tcttttcctt ccgcgaatgc ggtcagcaag cctttgtcac
    20041 gcgaaagatg cgccataatg cgcagttcaa tctgcgagta gtccgctgag acaatcacat
    20101 aatcctctgg cgcaataaac gcctggcgga tacgacgacc ttcttcgtta cgcaccggaa
    20161 tgttttgcag gttaggatcg gttgacgata aacgtcccgt tgcagttact gcctggtgat
    20221 aagaggtatg cacacgcccg gttttcgggt tgatcatcag cggcagcttg tcggtgtagg
    20281 tcgatttcag cttcgccaga ccacgatact ccagaatcac ttttggcaac ggatagtcca
    20341 gcgccagttc ttccagtacc tcttccgacg ttgacggcgc gccacccggc gttttcttca
    20401 gcggtttaat accctgtttt tcaaagagaa tggtttgtaa ctgcttggtg gaagaaaggt
    20461 taaattcctc acctgcaatt tcatgcgctt tcttttccag ctcagccaga cgaagggtga
    20521 gctcttcaga atgattgtgc agcactttcg gatcgatctt cacaccgtta cgttcaatgc
    20581 gtgaaagcac tggcaccagc ggcatttcga tattctcgaa gacgttcaac ggccctttgt
    20641 gtttttgcag atccggccac attttcagat gcaactgcaa ggtgacatct gcatcttcgg
    20701 cggcgtaacg tccggcttct tcgagggcaa tctggttaaa ggtcagttga tttttgcctt
    20761 taccagcaat ctcttcaaaa gtgatggttt tgtgcttcaa ccaacgttcc gcgaggctgt
    20821 ccatatcgtg acgcccggca acgctattga gaatgtagga ctccagcatg gtatcaaacg
    20881 caatcccacg cagttcaatg ccgtagttcg ccagaatacc gcgatcgtat ttcaggtttt
    20941 gcccgacctt cagcgccttt tcatcttcca gcagcggttt tagcaactcg agtgcacgct
    21001 cgcgagagat ttgatcgggc gcatcaagat aatcatgagc aaccggaata tatgccgcta
    21061 cgcctggctc gatagcaaaa gaaagcccga ccaggttagc agagatgtta tcaaggctgt
    21121 cggtttcggt atcaaatgca aataccggcg ctttttccag cttcgcaatc cacgctttca
    21181 gtgtttcttc atcaaggatg gtgacgtagt tgtcataaga aatcaccgtt gccgtcactt
    21241 ctggcgcttc gtctgcaaca ctggtttcct gcggcttcgc ggctggtttt gcccctttgg
    21301 cctgtaacca tttgcccgct tcgacatcag cagtccagcg tttgaactca tactttttga
    21361 acagccccaa caactcttcc gctgccggtt gctgcacttc cagttgttca caggtcagct
    21421 ccagttcaac gtcggtttta atcgtcgcca gctggtatga gagataagca acttctttgt
    21481 tttgctcgag cttcgctgcc attgttttcg cgccacggaa gctcaaccca gcaatttttt
    21541 ctggctcggc atacagcgta tccagcccgc caagaccttg cagcaatgcc tgcgcggttt
    21601 tttcaccgac gcccggtacg ccaggaatgt tatcggagga gtcacccatc agcgccagga
    21661 aatcgatgat cagttctggc ggcacgccgt acttattcac cacctcttcc ggtccgagga
    21721 tggtattcgt catggtattg ataagcgtaa tatttggcgt caccagctgc gccatatctt
    21781 tatcgccagt gctgatcagc accggacgcc cggctttttc ggcttcgcgc gccagagtac
    21841 cgataacgtc gtccgcttct acgccagaaa ccgccagcag cggcagtccc atcgctttaa
    21901 ccatcgcgtg cagaggttcg atttgtgctc gcagatcgtc cggcattggc gggcgatgtg
    21961 atttgtaatg ttcaaacagt tcatcacgaa aggtttttcc cttggcgtca aagaccaccg
    22021 ctgcatgcgt cggtttatat tgcatgatca gactgcgcag catgttgagg acaccataca
    22081 tcgcaccggt cggctcgcct gcgctgttag tcagcggggg aaacgcgtga tatgcgcgat
    22141 aaagataaga tgaaccatct acaaggataa gtggattttg ggggatctga accataatgt
    22201 ccgtgcctgt ttatcagatt atgggtaaag gatgccacag acaggatgaa aacatgagtt
    22261 tttcaccgct tttcgtgaaa atcttttgta gatcttctgg atcgctcgca aatcgtcatg
    22321 tggataactt tgtgcataaa ttatttacat cgctttta
//
LOCUS       DAZFRN010000073        22324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFRN010000073 DAZFRN010000000
VERSION     DAZFRN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.073"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..147)
                     /locus_tag="ACHKUB_002719"
     CDS             complement(<1..147)
                     /locus_tag="ACHKUB_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6655706.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSL"
     gene            complement(157..708)
                     /gene="ybcL"
                     /locus_tag="ACHKUB_002720"
     CDS             complement(157..708)
                     /gene="ybcL"
                     /locus_tag="ACHKUB_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO6655707.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(877..1209)
                     /gene="emrE"
                     /locus_tag="ACHKUB_002721"
     CDS             complement(877..1209)
                     /gene="emrE"
                     /locus_tag="ACHKUB_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO6655708.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1543..1857)
                     /gene="fliE"
                     /locus_tag="ACHKUB_002722"
     CDS             complement(1543..1857)
                     /gene="fliE"
                     /locus_tag="ACHKUB_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO6655709.1"
                     /translation="MSVIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2072..3730
                     /gene="fliF"
                     /locus_tag="ACHKUB_002723"
     CDS             2072..3730
                     /gene="fliF"
                     /locus_tag="ACHKUB_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO6655710.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3723..4718
                     /gene="fliG"
                     /locus_tag="ACHKUB_002724"
     CDS             3723..4718
                     /gene="fliG"
                     /locus_tag="ACHKUB_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO6655711.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4711..5397
                     /gene="fliH"
                     /locus_tag="ACHKUB_002725"
     CDS             4711..5397
                     /gene="fliH"
                     /locus_tag="ACHKUB_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO6655712.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5397..6770
                     /gene="fliI"
                     /locus_tag="ACHKUB_002726"
     CDS             5397..6770
                     /gene="fliI"
                     /locus_tag="ACHKUB_002726"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO6655713.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLQLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6789..7232
                     /gene="fliJ"
                     /locus_tag="ACHKUB_002727"
     CDS             6789..7232
                     /gene="fliJ"
                     /locus_tag="ACHKUB_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO6655714.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7229..8356
                     /gene="fliK"
                     /locus_tag="ACHKUB_002728"
     CDS             7229..8356
                     /gene="fliK"
                     /locus_tag="ACHKUB_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6655715.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8461..8925
                     /gene="fliL"
                     /locus_tag="ACHKUB_002729"
     CDS             8461..8925
                     /gene="fliL"
                     /locus_tag="ACHKUB_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO6655716.1"
                     /translation="MTDYAISKKSKRSLWIQILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8930..9934
                     /gene="fliM"
                     /locus_tag="ACHKUB_002730"
     CDS             8930..9934
                     /gene="fliM"
                     /locus_tag="ACHKUB_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO6655717.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9931..10344
                     /gene="fliN"
                     /locus_tag="ACHKUB_002731"
     CDS             9931..10344
                     /gene="fliN"
                     /locus_tag="ACHKUB_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO6655718.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10347..10712
                     /gene="fliO"
                     /locus_tag="ACHKUB_002732"
     CDS             10347..10712
                     /gene="fliO"
                     /locus_tag="ACHKUB_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO6655719.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            10712..11449
                     /gene="fliP"
                     /locus_tag="ACHKUB_002733"
     CDS             10712..11449
                     /gene="fliP"
                     /locus_tag="ACHKUB_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO6655720.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11459..11728
                     /gene="fliQ"
                     /locus_tag="ACHKUB_002734"
     CDS             11459..11728
                     /gene="fliQ"
                     /locus_tag="ACHKUB_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO6655721.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11737..12522
                     /gene="fliR"
                     /locus_tag="ACHKUB_002735"
     CDS             11737..12522
                     /gene="fliR"
                     /locus_tag="ACHKUB_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6655722.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12812..13435
                     /gene="rcsA"
                     /locus_tag="ACHKUB_002736"
     CDS             12812..13435
                     /gene="rcsA"
                     /locus_tag="ACHKUB_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO6655723.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13479..13667)
                     /gene="dsrB"
                     /locus_tag="ACHKUB_002737"
     CDS             complement(13479..13667)
                     /gene="dsrB"
                     /locus_tag="ACHKUB_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO6655724.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13830..14057
                     /gene="yodD"
                     /locus_tag="ACHKUB_002738"
     CDS             13830..14057
                     /gene="yodD"
                     /locus_tag="ACHKUB_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO6655725.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14355..15170
                     /gene="yedP"
                     /locus_tag="ACHKUB_002739"
     CDS             14355..15170
                     /gene="yedP"
                     /locus_tag="ACHKUB_002739"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO6655726.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15167..16861)
                     /gene="dgcQ"
                     /locus_tag="ACHKUB_002740"
     CDS             complement(15167..16861)
                     /gene="dgcQ"
                     /locus_tag="ACHKUB_002740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO6655727.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17032..17214)
                     /gene="yodC"
                     /locus_tag="ACHKUB_002741"
     CDS             complement(17032..17214)
                     /gene="yodC"
                     /locus_tag="ACHKUB_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO6655728.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17293..18210)
                     /gene="yedI"
                     /locus_tag="ACHKUB_002742"
     CDS             complement(17293..18210)
                     /gene="yedI"
                     /locus_tag="ACHKUB_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO6655729.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            18383..19303
                     /gene="yedA"
                     /locus_tag="ACHKUB_002743"
     CDS             18383..19303
                     /gene="yedA"
                     /locus_tag="ACHKUB_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO6655730.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAAVIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19292..19762)
                     /gene="vsr"
                     /locus_tag="ACHKUB_002744"
     CDS             complement(19292..19762)
                     /gene="vsr"
                     /locus_tag="ACHKUB_002744"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO6655731.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19743..21161)
                     /gene="dcm"
                     /locus_tag="ACHKUB_002745"
     CDS             complement(19743..21161)
                     /gene="dcm"
                     /locus_tag="ACHKUB_002745"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO6655732.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21228..21923)
                     /gene="yedJ"
                     /locus_tag="ACHKUB_002746"
     CDS             complement(21228..21923)
                     /gene="yedJ"
                     /locus_tag="ACHKUB_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO6655733.1"
                     /translation="MDLQHWQAEFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPHTMQTIRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(21963..22244)
                     /gene="drpB"
                     /locus_tag="ACHKUB_002747"
     CDS             complement(21963..22244)
                     /gene="drpB"
                     /locus_tag="ACHKUB_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO6655734.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5449 a         5900 c         5683 g         5292 t
ORIGIN      
        1 tagactatgc gtctcccaat tgataacttt ttttccctga cggatacgac ataaagctgg
       61 cataagaggc ttaaccctgt gaatctcatg atgtttatgc atccgtattt cttcaatctt
      121 taggttagtc ctatcttttg ccagcatact cttatcctac tttacctcat aaacgggtat
      181 tatctcagcg gttgcaattt tattggcatt aagcatataa ccaactaacg ctccgctgga
      241 attagaatcc acaggaatct tatcagtttt tagagcccat attttaaact ggtaatgatg
      301 gggtttatct cctttgggag gacatgctcc accaaatccc gcatagccaa aatcatttcg
      361 gccttggaca gcaccagtcg gaagttttgt accatcacgt cttcctgcat cagcgggcaa
      421 atatgttacc gttgctggaa tattagcaac agtccagtgc caccatccgc tgcctgtagg
      481 cgcatctgga tcatacacag ttacggcaaa gcttttggtg ccttctggag caccagacca
      541 ggttaatgag ggcgatgtat tacccccctc acacccaaat ccagaaaata catgagacgt
      601 tgtaagttgc tctcctgttt ttatttcatt actagtgacc tgaaatgctg cggcctgcgc
      661 agaaaatgtt atgaatgcca atacacttga aacgataagt tttttcataa aaacctcttt
      721 gttgtgacct atcgttattt tatttgatgt gcattgatct cattatgcaa aaaggcgcaa
      781 tgatcgcgca aatgcaatca taattataaa gttgccccga acattagcca taatatgcac
      841 aaatcagatt cctgttatct caaggatgat tattttttaa tgcggtgcgc ttcgtgacaa
      901 caagttaatg actaacacac cggcacaaat caacatcatg cctatgatgg ctggcaagtc
      961 cagtcgttgg ccaaaaaatc cccaggaaag caaactaatc agaacgatac cgactcctga
     1021 ccagatagca taagcaatcc ctgtaggtat ataagccagt gtctgagcta ataaccaaaa
     1081 tgatgcacaa tagcaaataa ttgtgccaac agagggccat aaccgtgtaa acccctctga
     1141 aaacttcatt aaggttgtac caatgacctc tgcaagtatt gcgccaccaa gataaatata
     1201 agggttcata gcatattctt tcctgttcat actggagaga attgtactac agtttgaaca
     1261 caactcaccg ttttcatcct ggtgtatgtg tatccagggg gctctggcgt ggcaagagat
     1321 aaaggcagga acaataatga tcagaaggaa ccccaggcta acaaaagagt tcactcttcc
     1381 tatgagcaaa gcgaagacaa aacatgttat cgcgtttacg gatcgtggat ggcagaaaac
     1441 tttcaggatc aggtatccat tttaaaccag aaatagggtg agtttgcccc atccatgccc
     1501 cacgcgggca gatgggatgt aattaccaac aggttacaaa acctacacct gcatggacat
     1561 cacttcctga tacgccgcca ccagcttatt acgcacctga atccccattt gcatagaaac
     1621 tgaggctttt tgcatatcgg tcatcacatc gtttaacgcc acgccgggtt caccgagggt
     1681 gaatttttct gcctgcgtgc gggcggctgt ttgtgtatcg cttatgcgat cgagcgcggc
     1741 gtgcagctgc ccggcaaaac taatggtcgg ttgtggcagt gattcctgtg cacgcgcact
     1801 catcgccgta gcctgtaact ggctgataac cccttcaatc ccctgtatca ctgacatttt
     1861 cgtctcccgg ataatttctg ttagcaaagc ctaccagtaa gtcaataaga caaaggcgct
     1921 aaatagcaac aaaaaaacgg gtttattggc ggatagaaaa aaacgaaagc acaaataatg
     1981 ggagcgtcaa tttttcgagt ttgctgaccc gggagtgagt cttgttccac tttgccaata
     2041 acgccgtcca taatcagcca cgaggtgcgc gatgaatgcg actgcagccc agacaaaatc
     2101 tcttgagtgg cttaatcgcc tgcgtgcgaa tccgaaaatt ccattgattg ttgccggttc
     2161 cgcggcagtg gcggtcatgg tcgcactgat cctgtgggcg aaagcccccg actaccgcac
     2221 attattcagc aatctttccg atcaggatgg tggcgcaatt gtcagccaac tgacgcaaat
     2281 gaatattcct taccgcttca gcgaagccag cggcgctatt gaagttccgg cagataaagt
     2341 tcacgaactg cgtctgcgcc tggcacaaca aggtttgccc aaaggcggcg cggtcggttt
     2401 cgaactactc gatcaggaaa agtttggtat cagccagttc agcgaacagg tgaattatca
     2461 gcgggcgctg gaaggcgagc tttctcgtac catcgcaact atcggcccgg taaaaggggc
     2521 gcgcgtacat ctggcaatgc cgaaaccgtc tttattcgtc cgtgaacaaa aatccccttc
     2581 tgcatcggtg acggtaaatc tgttacccgg ccgcgcactc gatgaagggc aaattagcgc
     2641 cattgtgcat ctggtttcca gcgccgttgc tggtctgccg ccgggaaacg tcacgctggt
     2701 ggatcagggc ggacatctgt taacccagtc caataccagc gggcgcgatc ttaatgatgc
     2761 tcagttgaaa tatgccagcg atgtcgaagg ccgtattcag cggcgtattg aagcaatcct
     2821 gtcgcctatt gttggtaacg gtaatattca cgcccaggtc acggcgcagc tggacttcgc
     2881 cagtaaagaa caaacggaag aacagtatcg ccctaacggt gatgaatctc atgcggcgct
     2941 tcgttcacgc cagcttaatg agagcgagca aagcggttcc ggttatccgg gcggcgtacc
     3001 gggggcgttg tcgaatcaac cggcacctgc gaataacgcg ccaatcagca cgcctccggc
     3061 aaatcaaaat aaccgccagc agcaggcgag caccaccagc aatagcgggc cgcgtagcac
     3121 acagcggaat gaaaccagta actacgaagt cgatcgcacc attcgtcata ccaaaatgaa
     3181 cgtgggcgat gtgcaacgtc tgtcagtcgc ggtagtggtg aattacaaaa ccttgccaga
     3241 cggtaaacca ttgcctctca gcaacgaaca gatgaagcaa attgaagatc tgacccgcga
     3301 ggcgatgggc ttttctgaaa aacgcggtga ctcgctcaat gtcgttaact cgccgttcaa
     3361 tagcagtgac gaaagcggcg gagaactgcc gttctggcaa cagcaagcgt ttatcgatca
     3421 gttgctggct gccggtcgct ggttgctggt actgctggtg gcgtggctgc tgtggcggaa
     3481 agcggtacgc ccgcagttaa cacgtcgcgc cgaggcgatg aaagctgtac agcaacaggc
     3541 gcaggcccgt gaggaagtgg aagatgcggt ggaagtccgc ctgagcaaag acgaacaact
     3601 ccaacaacga cgcgctaacc aacgtctggg ggcagaagtc atgagccagc gtatccgtga
     3661 aatgtctgat aacgatccgc gcgtggtggc gctggtcatt cgccagtgga taaataacga
     3721 tcatgagtaa cctgacaggc accgataaaa gcgtcatcct gctgatgacc attggcgaag
     3781 accgggcggc agaggtgttc aagcacctct cccagcgcga agtgcaaacc ctgagcgctg
     3841 caatggcgaa cgtcacgcag atctccaaca agcagctaac cgatgtgctg gcggagtttg
     3901 agcaagaagc tgaacagttt gccgcactga atatcaacgc caacgattat ctgcgttcgg
     3961 tattggtcaa agctctgggt gaggaacgtg ccgccagcct gctggaagat attctcgaaa
     4021 ctcgcgatac cgccagcggt attgaaacgc tcaactttat ggagccgcag agtgccgccg
     4081 atctgattcg cgatgagcat ccgcaaatta tcgccaccat tctggtgcat ctgaagcgcg
     4141 cccaggccgc cgatattctg gcgttgttcg atgaacgtct gcgccacgac gtgatgttgc
     4201 gtatcgccac ctttggcggc gtgcagccag ccgcgctggc ggaactgacc gaagtactga
     4261 atggcttgct cgacggtcag aatctcaagc gcagcaaaat gggcggcgtg agaacggcag
     4321 ccgaaattat caacctgatg aaaactcagc aggaagaagc cgttattacc gccgtgcgcg
     4381 aattcgacgg cgagctggcg caaaaaatta tcgacgagat gttcctgttc gaaaatctgg
     4441 tggatgtcga cgatcgcagc attcagcgtc tgttgcagga agtggattcc gaatcgctgt
     4501 tgatcgcgct gaaaggggcc gagcagccac tgcgcgagaa attcctgcgc aatatgtcgc
     4561 agcgtgccgc cgatattctg cgcgacgatc tcgccaaccg tggtccggtg cgtctgtcgc
     4621 aggtggaaaa cgaacagaaa gcgattctgc tgattgtgcg ccgccttgcc gaaactggcg
     4681 agatggtaat tggcagcggc gaggatacct atgtctgata atctgccgtg gaaaacctgg
     4741 acgccggacg atctcgcgcc accacaggca gagtttgtgc ccatggtcga gccggaagaa
     4801 accatcattg aagaggccga acccagcctt gagcagcaac tggcgcaact gcaaatgcag
     4861 gcccatgagc aaggttatca ggcggggatt gccgaaggtc gccagcaagg tcatgagcag
     4921 ggctatcagg aaggactggc ccgggggctg gagcaaggtc tggcagaggc gaagtctcaa
     4981 caagcgccaa ttcatgcccg gatgcagcaa ctggtcagcg aatttcaaac tacccttgat
     5041 gcgcttgata gtgtgattgc ttcgcgcctg atgcagatgg cgctggaggc ggcacgtcag
     5101 gtgatcggtc agacgccaac agtggataac tcggcactga tcaaacagat ccaacagttg
     5161 ttgcagcaag aaccgttatt cagcggtaaa ccacagctgc gcgtgcaccc ggatgatctg
     5221 caacgtgtgg atgatatgct cggcgcaacc ttaagtttgc atggctggcg cttgcggggc
     5281 gatcccacac tccatcctgg cggctgtaaa gtctccgccg atgaaggcga tctcgacgcc
     5341 agtgtcgcca ctcgctggca agaactctgc cgtctggcag caccaggagt ggtgtaatga
     5401 ccacgcgcct gactcgctgg ctaaccacgc tggataactt tgaagccaaa atggcgcagt
     5461 tgcctgcggt acgtcgctac gggcgattaa ctcgcgctac cgggctggtg ctggaagcca
     5521 ccggattaca attgcagctc ggcgcaacct gtgtcattga gcgccagaac ggcagcgaaa
     5581 cgcacgaagt agaaagcgaa gtcgttggct ttaacggtca acggctgttt ttaatgccgc
     5641 tggaggaagt ggaaggtgtc ctgcccggcg cgcgggttta tgccaaaaac atttcggctg
     5701 aaggactgca aagcggcaag cagttaccgc tcggtccggc gttattaggt cgtgttctgg
     5761 acggcagcgg taaaccgctc gatggcctcc cctcccccga tacgacggaa accggtgcgc
     5821 tgattacccc gccatttaac ccgttgcaac gtacaccgat tgaacatgtg ctggacaccg
     5881 gcgtgcgccc aatcaatgcc ctgcttaccg ttgggcgtgg gcagcgtatg gggctgtttg
     5941 ccgggtccgg cgttggtaaa agtgtgctgc tggggatgat ggcacgttac acccgcgccg
     6001 atgtcattgt cgtgggtttg attggtgaac gcggacgcga agtaaaagat tttattgaga
     6061 atatcctcgg tgccgaaggg cgtgcacgct ccgtggtgat tgccgctccg gcggatgttt
     6121 ctcctctcct gcgaatgcag ggtgccgcct atgccacgcg cattgccgaa gattttcgcg
     6181 atcgtggtca gcatgtgttg ctgattatgg actccctcac ccgctacgcg atggcccagc
     6241 gtgaaattgc gctggcgatt ggcgaaccac ctgccactaa aggttatcca ccgtcggtgt
     6301 ttgccaaatt accggcactg gtcgagcgtg ccggaaatgg aattagcggc ggcggctcga
     6361 ttaccgcgtt ttataccgtg ctcaccgaag gcgatgacca gcaggacccc attgccgact
     6421 ctgcgcgggc aatcctcgac ggtcacattg tgctgtctcg ccgactggcg gaagcagggc
     6481 actatccggc tatcgatatt gaagcgtcga tcagccgcgc aatgacggcg ttgatcagtg
     6541 agcaacatta cgcgcgagtg cgcaccttca aacagctgtt gtcgagtttt cagcgtaacc
     6601 gcgatctggt tagcgtcggc gcgtatgcca aaggcagcga tccgatgctc gacaaagcca
     6661 tcgccctgtg gccgcagctg gagggctatt tgcaacaagg catttttgaa cgcgcggact
     6721 gggaagcgtc tctccagggg ctggagcgta ttttcccgac agtgtcataa cccaggagat
     6781 aacggcagat ggcagaacat ggcgcgctgg cgaccctgaa agatctggca gaaaaagagg
     6841 tagaggatgc cgcgcgcctg ctgggtgaaa tgcgtcgcgg gtgtcagcag gcggaagaac
     6901 agctcaaaat gctgatcgat tatcagaatg aatatcgcaa taacctcaac agcgatatga
     6961 gtgccgggat gaccagcaac cgctggatca actatcagca gtttatccag acgttggaaa
     7021 aagccattac tcagcatcgc cagcaactta atcagtggac gcagaaagtt gacattgccc
     7081 tgaacagctg gcgagaaaag aaacaacgtt tgcaggcctg gcagacactg caggaacggc
     7141 aatccacggc ggcactgctt gcagaaaacc gcctcgatca gaaaaagatg gatgagttcg
     7201 cccagcgcgc cgccatgagg aaacctgaat gattcgctta gcgcccttga ttaccgccga
     7261 cgttgacacc accacattgc ctggcggcaa agccagcgat gctgcacaag attttctcgc
     7321 gttgttgagc gaagcattag caggcgagac aactaccgac aaagcggccc cccagttgct
     7381 ggtggcaaca gataagccca cgacaaaagg cgagccgctg gtcagcgaga ttcttgccga
     7441 tgcgcaacaa gcggatttac tgatccctgt ggatgaaaca ccgcctgtca tcaacgacga
     7501 acaatccaca tcaacaccat taaccaccgc tcaaacgatg acgttggctg cggtggctgg
     7561 caacaatacg gcaaaagacg aaaaagcgga tgatctgaat gaagacgtca ccgcaagcct
     7621 gagcgccctt tttgcgatgt tgccgggttt tgacaatacg cccaaagtga ctgatgtgcc
     7681 gtcaaccgtg ttaccggcag agaaaccaac gctcttcaca aaactgactt ctgcgcaact
     7741 cacaacagca cagcctgatg atgcccccgg cacgccagct cagccattaa caccgctggt
     7801 agcagaagcc cagagtaaag cggaagtcat cagcacacct tcaccggtga ccgctgccgc
     7861 cagcccgcta atcactccac accagacaca gccactgccc accgtcgccg cgcctgtgtt
     7921 gagtgcaccg ctgggttctc acgaatggca acaatcatta agccagcata tttcgctgtt
     7981 cacccgccag gggcaacaaa gtgcagagtt gcgtctgcac ccgcaggatt taggtgaagt
     8041 gcaaatctcc ctcaaagtgg atgataacca ggcgcaaatc cagatggttt caccgcatca
     8101 acacgtacgc gccgccctgg aagcagcgct tccggtactg cgaacgcagc tggccgaaag
     8161 tggcattcag ttagggcaaa gcaacatcag tggcgaaagc tttagtggtc agcagcaggc
     8221 cgcttcccaa caacagcaaa gccaacgcac agtaaaccat gaacctctgg cgggggaaga
     8281 agacgatacg cttccggttc ccgtctcttt acaagggcgc gtaacaggca acagcggcgt
     8341 tgatattttc gcctaacgtc agaggtagca ccgtaatccg cgtcttttcc ccgctttgtt
     8401 gcgctcaaga cgcaggataa ttagccgata agcagtagcg acacaggaag accgcaacac
     8461 atgactgatt acgcgataag caagaaaagc aagcgatcgc tttggatcca gattctggta
     8521 ttcattaccc tcgcggcctg tgccagcgca ggttacagct actggcattc gcatcaggtt
     8581 gccgctgaag acaaagcgca gcaacgcgtc gtgccctccc cggtcttcta tgcgctggat
     8641 acctttacgg tcaatctggg cgatgcagat cgcgtacttt atatcggcat aaccctgcgc
     8701 ctgaaagatg aagctacccg ctcgcggctg agtgagtatt tgccggaagt ccgtagccgt
     8761 ttgctgttac tgttttcgcg tcaggatgct gccgtactgg cgacagaaga aggcaagaaa
     8821 aacctgattg ccgagattaa aaccacgctt tccaccccgc ttgttgccgg gcaaccgaaa
     8881 caggatgtca ccgacgtgct gtataccgct tttattctgc gataacgaca tgggcgatag
     8941 tattctttct caagctgaaa ttgatgcgct gttgaatggt gacagcgaag tcaaagacga
     9001 accgacagcc agtgttagcg gcgaaagtga cattcgtccg tacgatccga atacccaacg
     9061 acgggttgtg cgcgaacgtt tgcaggcgct ggaaatcatt aatgagcgct ttgcccgcca
     9121 ttttcgtatg gggctgttca acctgctgcg tcgtagcccg gatataaccg tcggggccat
     9181 ccgcattcag ccgtaccatg aatttgcccg caacctgccg gtgccgacca acctgaacct
     9241 tatccatctg aaaccgctgc gcggcacagg gctggtggtg ttttcaccga gtctggtgtt
     9301 tatcgccgtg gataacctgt ttggcggcga tggacgcttc ccgaccaaag tggaaggtcg
     9361 cgagtttacc cataccgaac agcgcgtcat caaccgcatg ttaaagctgg cgcttgaagg
     9421 ctatagcgac gcctggaagg cgattaatcc gctggaagtt gagtacgtgc gttcggaaat
     9481 gcaggtgaaa tttaccaata tcaccacctc gccgaacgac attgtggtta acacgccgtt
     9541 ccatgtggag attggcaacc tgaccggcga atttaatatc tgtctgccat tcagcatgat
     9601 tgagccgcta cgggaattgt tggttaaccc gccgctggaa aactcgcgta atgaagatca
     9661 gaactggcgc gataacctgg tgcgccaggt gcagcattca cagctggagt tggtcgccaa
     9721 ctttgccgat atctcgctac gcctgtcgca gattttaaaa ctgaaacccg gcgacgtcct
     9781 gccgatagaa aaacccgatc gcatcatcgc tcatgttgac ggcgttccgg tactgaccag
     9841 tcagtacggc accctcaacg gtcagtatgc gttacggata gaacatttga ttaacccgat
     9901 tttaaattct ctgaacgagg aacagcccaa atgagtgaca tgaataatcc ggccgatgac
     9961 aacaacggcg caatggacga tctgtgggct gaagcgttga gcgaacaaaa atcaaccagc
    10021 agcaaaagcg ctgccgacgc ggtgttccag caatttggcg gtggtgatgt cagcggaacg
    10081 ttgcaggata tcgacctgat tatggatatt ccggtcaagc tgaccgtcga gctgggccgt
    10141 acgcggatga ccatcaaaga gctgttgcgt ctgacgcaag ggtccgtcgt ggcgctggac
    10201 ggtctggcgg gcgaaccact ggatattctg atcaacggtt atttaattgc ccagggcgaa
    10261 gtggtggtcg ttgccgataa atatggcgtg cggatcaccg atatcattac tccgtctgag
    10321 cgaatgcgcc gcctgagccg ttagtgatga ataaccacgc tactgtgcaa tcttccgcgc
    10381 cggtttctgc tgcgccactg ctgcaggtga gcggcgcact catcgccatt attgccctga
    10441 tcctcgctgc tgcctggctg gtaaaacggt tgggatttgt ccctaaacgc actggcgtta
    10501 acggtctgaa aattagcgcc agtgcttcgc tgggcgcgcg tgaacgggtt gtggtggtcg
    10561 atgtggaaga tgcacggctg gtgctcggcg ttaccgcagg gcaaatcaat ctgctgcata
    10621 aacttccccc ttcagcacca acggaagaga taccgcagcc cgattttcag tcggtcatga
    10681 aaaacttgct taagcgtagc gggagatcct gatgcgtcgt ttgttgtctg tcgcccctgt
    10741 ccttctctgg ctgattacgc ccctcgcctt cgcgcaactg ccgggtatca ccagccagcc
    10801 gctgcctggc ggtggacaaa gctggtcgct cccggtgcag acgctggtgt tcatcacctc
    10861 gttgacgttt attccggcaa ttttactgat gatgaccagt ttcacccgca tcattattgt
    10921 ttttggttta ttgcgtaacg cgctgggaac accctccgcg ccacctaacc aggtattgct
    10981 ggggttggcg ctgtttttga ccttttttat tatgtcgccg gtgatcgaca aaatttatgt
    11041 cgatgcgtac cagccattca gcgaagagaa aatatcaatg caggaggcgc tggaaaaagg
    11101 ggcgcagccg ctgcgtgagt ttatgctgcg tcagacccgt gaggcggatt tagggttgtt
    11161 tgccagactg gcgaatactg gcccgttgca gggacctgaa gccgtgccga tgcgcatttt
    11221 gctcccggcc tacgtgacca gcgagttgaa aaccgcattt cagataggct tcacgatttt
    11281 catccctttt ttgattatcg acctggtgat tgccagcgtg ttgatggcat tggggatgat
    11341 gatggtcccc cccgccacca ttgctctgcc ctttaaactg atgctgtttg tactggtgga
    11401 tggctggcaa ttgctggtcg gttcgctggc gcagagcttt tacagctaga gaggcaaaat
    11461 gacacctgaa tcggtcatga tgatggggac tgaagcgatg aaagtcgcgc tggcactggc
    11521 tgccccgctg ttgctggtgg cgctggtcac gggccttatc atcagtattt tgcaggccgc
    11581 cacgcagatc aacgaaatga cgctgtcgtt tattccaaaa atcatcgccg tatttattgc
    11641 cattattatt gccggaccgt ggatgctcaa tctgttgctg gattacgtcc gcaccttgtt
    11701 cactaacctg ccgtatatca tcgggtagcc cgtacaatga tgcaggtgac aagcgatcaa
    11761 tggctttcct ggctaagtct gtacttctgg ccgttactgc gcgtgctggc gctcatttct
    11821 accgcgccaa ttctgagcga acgcagcgtg ccgaaacggg taaaactggg tctggcaatg
    11881 atgatcacgt tcgctattgc cccatcatta cctgccaacg atgttcctgt tttttcgttc
    11941 tttgctctgt ggctggccgt gcagcagatc ctgatcggca ttgcgcttgg tttcaccatg
    12001 caatttgcct ttgccgctgt gcgaaccgct ggcgaaatta tcggtctgca aatggggctg
    12061 tcatttgcga cgtttgtcga tccggccagc catctcaata tgcccgttct agcccgtatc
    12121 atggatatgc tggcgttatt gctgttcctg acatttaacg gtcatttatg gttgatttca
    12181 ctgctggtcg atacctttca caccctgcca attggtggcg aaccgttgaa cagcaatgcg
    12241 tttctggcac tcaccaaagc agggagtttg attttcctta acgggctaat gctggcgtta
    12301 ccgctcatta ctctgctgct gacactgaat ctggcattag gtttacttaa tcgtatggcc
    12361 ccgcaattat ccatttttgt tattggattt ccattaactc tgactgtcgg tatctcttta
    12421 atggcggcat taatgccgtt aattgcacct ttttgcgaac atttattcag tgagatgttt
    12481 aatttgctgg ctgatattat tagtgaattg ccattaatat aattccgtaa cgtttatcat
    12541 gttatcctaa ggattatccg aaaaataata cctacgaaca tcttccagga tactcctgcg
    12601 gcgaaatatt tgttttaagc tcactcacat atcgcaacat ttactttact ttaagacaat
    12661 tccaggcaaa ttatacaaca ctttacggga tagtaagtcc gcctgaaaaa tcgcgagagt
    12721 ggcgcattag gtgacccatg ttgttccgtt tagtcatgat gaaatattca ggtaagggga
    12781 attatcgtta cgcattgagt gagggtatgc catgtcaacg attattatgg atttatgtag
    12841 ttacacccga ctaggtttaa ccgggtatct gttgagtaga ggggttaaaa aaagagaaat
    12901 caacgacatt gaaaccgttg atgaccttgc catagcttgt gattcacagc gcccttcagt
    12961 ggtgtttatt aatgaggact gtttcatcca tgatgcttct aacagtcagc acatcaagca
    13021 catcattaat caacatccca atacgttatt tatcgttttt atggcaattg ccaatgttca
    13081 ttttgatgaa tatctattgg tcagaaaaaa tttattgata agttctaaat cgattaaacc
    13141 ggaatctctc gacgatatcc ttggcgatat tctgaaaaaa gagacaacga taacctcgtt
    13201 tttaaatatg ccgacgttat cattgagccg aaccgaatcg agtatgttgc gaatgtggat
    13261 ggcaggtcag ggaaccattc aaatctctga ccaaatgaat atcaaagcca agaccgtttc
    13321 atcgcataaa ggtaatatta aacgtaagat caaaacgcat aataaacagg ttatctacca
    13381 tgtcgtccga ctgacggata atgtgactaa tggtattttt gtcaacatgc gctaacacat
    13441 tctgactggt ggtttcccac cagtcaggct gaattagatt actctgcttt ctccacaaag
    13501 ataccgtcct gatgccctgc ttcattaaag aaccagatgc cgagcgggta gtcttccagc
    13561 gaaaccaggt acattgtgcc ttcactaaac tcctcaactg ccagtaccac gccaggacga
    13621 cgcggaccgc catccgtttt gactgttacc cgatcattca ccttcatcgt tttcctcctg
    13681 tggctttgtg ccagtgtaga acaatttcgt ttttctggca gcgccaggcg cgcgcgagtg
    13741 ctgattttct cgacggtcta tacttaagag atgccaggcg gacttaacga ctggcggcaa
    13801 caacagagta acggttgcga ggaaagatga tgaaaaccgc aaaagagtac agcgataccg
    13861 caaaacgtga ggtcagcgtc gatgtcgatg ccctgctggc ggcgatcaat gaaattagcg
    13921 aaagcgaagt tcatcgcagc cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat
    13981 atcatacatg gcgtgaattg gcggatgcct tcgaactgga tattcatgac ttcagcgtct
    14041 ctgaagtgaa tcgttgaatg cacaataaaa aaatcccgac cctgaggggg tcgggatgaa
    14101 acttgcttaa gcaagaagca cttaaaaaat tcgttacacc aggaaatctg atgtgttcat
    14161 caccttatcc gcaatttttt tcgctgacaa gaaaatattc gcgatatgaa tgattaacgc
    14221 tacttatgaa tagaaatgtg acgtgcgtca ccccttggtg gctctggcgt caacgggatc
    14281 gctcccgaaa aaatcatctt tgattatttg tgctgttttg agaatcatcc tggtaagggg
    14341 agaactgcgc gatcatgttt tcaattcaac aaccactact ggtttttagc gatcttgatg
    14401 gcaccctgct ggacagtcat agttatgact ggcaaccggc tgccccctgg ctcagccgtt
    14461 tacgcgaagc gaatgttccc gtcattctct gtagcagtaa aacttcagcg gaaatgctgt
    14521 acttgcaaaa aacgttgggg ctacaagggt taccactgat tgccgaaaat ggcgcagtga
    14581 tccagcttgc tgaacaatgg caggatatag acggttttcc acgcatcatc tcaggtatta
    14641 gccatggcga aatcagccag gttttaaata cgctacgcga gaaagaacat tttaaattca
    14701 cgactttcga tgatgtcgac gatgcaacca tcgccgaatg gacgggatta agccgtagcc
    14761 aggcggcgct gacgcagctg catgaggcgt cggtaacgct aatctggcgc gacagtgacg
    14821 agcgtatggc acaatttacc gctcgtctga acgaactggg cttacagttt atgcagggtg
    14881 cgcgcttctg gcacgtcctg gatgcctctg ccggaaaaga tcaggccgcc aactggatta
    14941 tcgcgaccta tcaacaatta tcaggcaaac gcccaaccac acttggcctg ggcgatgggc
    15001 caaacgatgc gcccttactg gaggtaatgg attacgcggt gattgtgaaa gggctaaacc
    15061 gtgaaggggt gcatctgcat gatgaggatc cgacccgcgt ctggcgaacg cagcgtgaag
    15121 gaccggaagg ctggcgtgaa gggctggacc attttttctc cgctcgttaa gcgttatcgc
    15181 tcgcgcatac ccgattacgc ccggcctgtt tagcgagata aagccgacgg tcagccagtg
    15241 actggagttg ttcaaaatca taatcaccgg tttcctcgct gctactaacc cccaacgagg
    15301 cactgatgcg tatcgtcgta ctcttagcaa tcaacatctc tttttcattt aacttcaggc
    15361 gaatacgttc tgcgacttcc gcagcctccg tcagactcgc gcctggcaga atcacacaaa
    15421 actcctcacc accgacccgc ccggcaacgt cctgcgcacg caaggaactg ctaattaatc
    15481 cggcagcatg agaaagaaca cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt
    15541 taaaatggtc aagatcgacc tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt
    15601 tagcgagcgg acgggctttt tcgaacagtg cgccacgatt atataaacgg gttaaggtgt
    15661 cgtgccacgc ctgccactgc aacgagcttt gcagaacata catgttgctg accatccggc
    15721 gaatcacata ccaggagatg agtaacatgg tggtaaagag cgcccacagc agggttaatg
    15781 caatgctgat actaccgaaa tcgccgcgca cgccttcgct tagcgtatgg acgcgcacca
    15841 gcacaccgtc gaaatggtcc agacgttccc agctaacata gcgactgtcc atacgaatgc
    15901 cgccccgcgt gtcatgttcc atcgcctgcg ccagcaaggc cagttcacga ggatcaaaaa
    15961 tattccctgt tggatggtca ggattggaag aggtcaaaaa tctcagctta ctgtcataga
    16021 gctgatactc accatcgagg tttttatcga tggcgtttct taaaaattgc tgcatagtac
    16081 gcacaggaat actcatcccc agcacgccat accagtagtt attactgtct accggaacac
    16141 tgacggtaac ctgcggttca gtattgctgg cgtgttccgg ttgcgaagta aaccagcgta
    16201 ccgcgcggtg acgattttct cgttgcgaat ggccgataaa ccagggttgg gtgacatagc
    16261 cgtaataacg cgttggaaca ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc
    16321 cggcacgcga gacatacatc gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca
    16381 gcaagtaacc aacttccagt gcagcggtaa tttcattgtc gaggctttca ttttcgcgag
    16441 acaggagatt cccctcgctg actaatgcat ccgacacacc attaactggc agagtgcgcc
    16501 gtcggttgag ttcaatttgc caggcgtgct cgtcgcgatg ctgttcgaac tcggttacag
    16561 cattacgcag tgaagtgaaa tccagtggcg ctacgagagc ttcgcgcatg ccattacgca
    16621 aaaagatcag tttgtcgacg ttatactgca aatgcttatc gagcgcgttg gctacgtttt
    16681 ccagatgatt acgctggctg gagatatagg catcttccag caccactact tcacgccagg
    16741 tgagcaaggt ggaaaaaagc aataccacga taaagcagag attaacgaca tgaccaggcc
    16801 ccaggcggcg tgcgagtttt ttcaaccagc tctggttttc catttttgtc tcgtgctgca
    16861 ccctgacgac tcccaacctg cttttttatg attctggcat aacctggcga tagcgtaccg
    16921 caaaacagcg ataattttat aatggtggca tgataagacg cgactatcat tcattgcagg
    16981 cattggcaca ccaggccgga tgtgatccgg cctggtgtta atgataccct ttcaaacttc
    17041 ttccgcagaa cgactcccct cccccggaac aagctcggtt tcatgaaaag cttcccgctt
    17101 gaccccgtaa ccgtcatacc aacgacactc aaccataccg ctggagtatc cggtgacaat
    17161 catccgcggg ccgccctctt taaccgtaac ttcctcacta accataaagc tcatactcgc
    17221 ctcctttttt cgagtgaaac ttgttcacct tagttgaaga tggcgaattt tgcataatag
    17281 cctgtgcgag tattaatgtg cctgaccgcg tattttcgct acggctttca ctcccagcac
    17341 cacgatgccg ccgatgataa atccaagaat cagatttaaa acagtcggta atatcatcgc
    17401 caccactgca ctttgctgcc cggcgaaatg ttcaatggcg tgatgcagcg gcgcaattcc
    17461 atgtaccaca atcccaccgc cgacgaggaa catcgccagc gtgccgacaa tcgataacgc
    17521 cttcatcaac cagggcgcaa tgatcaataa ccctttacct aatgcctgca tcagcgcgct
    17581 ggatttttcc gccagccaat accccaggtc atcaatctta acgataaccc ctaccagacc
    17641 gtagacgccc acggtcacta ccagcgcgat gcctgaaagc accagcacct gattaagcaa
    17701 cggcgcttcg gccacaatcc ccagcgtgat ggcgacgatt tcggcagaca atataaaatc
    17761 ggtacgaatc gcccctttta ttttgtcctt ttcaaacttc agcggatcct gcgccgccag
    17821 cttctccaga cgctgctggc tctgcgccgg atcttcttta tgtttacgcg cctccagcat
    17881 atgcagcact ttctctactc cttcaaagca gagaaacgcg ccaccaatca tcaacagagg
    17941 cgtaatcgcc cacgggataa acgcactgat gatcagcgcc agcggcacca gaatcacttt
    18001 attaatcagc gatcctttcg ccacgcccca gaccacggga agttcccggt tggcccgcac
    18061 gcctgaaact tgttgcgcat tgagcgataa gtcatcccct aatacaccgg cggttttctt
    18121 cgccgccagt ttgcccatca cggagatatc gtccagtagt gtggcgatat catcgagcaa
    18181 cgtcagtaaa ctactcccgg ccaaaagcat tccctctcat ttttattgtt gaagggtgta
    18241 agtatgaagc aaaaacggca gctctgatac ttgcttcaca ggtcaacatt tttttaacaa
    18301 tcaagtgcaa ttatatctct cgccagcaga atgattttca cgtttactat gaaccacctt
    18361 tttatttcca ccgtgaggga ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct
    18421 gtttgcgttg tatatcattt ggggctcaac ctattttgtc attcggattg gcgtggaaag
    18481 ctggcctccg ttaatgatgg cgggcgttcg attcctggca gccggtattt tattgctggc
    18541 atttttgcta ctgcgcggac acaaactccc cccgctacgt ccgctactca atgccgcggt
    18601 gattggcctg ttattgctgg ctgtcggtaa tggcatggtg acggttgccg agcatcaaaa
    18661 tgttccttcc ggcatcgccg ccgttgtggt tgcaaccgtg cccctcttta ccctgtgctt
    18721 cagccgcctg tttggcatta aaacgcgcaa actggaatgg gtgggtatcg ccattgggct
    18781 tgccggaatc atcatgctca atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat
    18841 tctgatttta atcggctcga ttagctgggc gtttggctcg gtttatggct cgcgcattac
    18901 cttacctgta gggatgatgg cgggtgctat tgagatgctg gcggcaggcg tggtgttaat
    18961 gatcgcgtcg atgattgcag gtgaaaaact gacggcgctc ccttcccttt caggcttcct
    19021 tgcggtcggc tatctggcgc tgtttggttc gattatcgcc atcaacgctt atatgtattt
    19081 aatccgtaat gtcagtccgg ctctcgccac cagctacgcc tacgttaacc cggtggtcgc
    19141 ggtcttgctg ggtacgggac tgggcggaga gacgctgtcg aagattgaat ggctggcgct
    19201 cggcgtaatt gtcttcgcgg tggtgctggt cacgttggga aaatatctct ttccggcaaa
    19261 acccgtagtt gcgccagtta ttcaggacgc atcaagcgag taaatgaatc ccctgcgtgt
    19321 cgatctgcgc gctggcacct tcgccgcaga tccactcttc cagacgctcg ctaagcgctt
    19381 catccgtcag cttctcgcgc ccacgtaacg cgcactccca gacaatcaat acgcgccagc
    19441 cgagttcctg caagcgactg atatcgcggc gatcgcgctc aacattttta cctatcttct
    19501 ccagccagaa ctcggttcga gtcgcaggca ctttaaacag atagcagtga tgatgatgcc
    19561 agaagcagcc gtgggtaaat atcacgcagc gatattcatc aacgacaaaa tccgggcgtc
    19621 cgggcagact ggcgtcctga acgcgaaacg ccaggccctg cccggttaac agactggcga
    19681 ggcgcttctc tatcgccgta tcacgcgtgg caatcgcgcg catatttttg ctgcgagtgg
    19741 ccttatcgtg aacgtcggcc atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg
    19801 atttttggct caagcagttt tgccaccgcg gcaaagaccg gcacgaccac cgagttaccg
    19861 aactggcgat aggcctgagt gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct
    19921 tcaaaaccca ttaagcgcgc gcattcccga ggcgttaacc gacgtggacg atgttgctga
    19981 ttcagcggat cgtcaaagtc tttctcaccc gtggccatat cccagccacg atcgattaaa
    20041 atttccgcgc catctttgta ataacgcgca gacagcgtac gggtgacgct ttgcggattg
    20101 ttcggataaa ccattccata accgaagccg ttaccgcgcg cctgatgttt tttcgcatat
    20161 cgatagaggt acttccacag caccggcgtc aggatatatt tcgcctcgac catcgggtcc
    20221 agcagctgcg ccagcgtcac tcgctgcgca gggaaacatt cgctgatatc acgcagggta
    20281 aaatcggctt tcagattcag atcgcgacga aaacccacca gcacgatgcg ttcacggtgc
    20341 tgcggcagaa aatgtttacc atcgatgatt ttcggatcgt ccggcccgtt atcttctgca
    20401 tcagccacgt catagcccag ttcgtccagc gtctgcatga tgatgcggaa cgttttaccc
    20461 tggtcgtggc ttttcaggtt tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg
    20521 tcgataatgc gcaccacatc aaagaacagc gtgccctgag tatcgcaggc aaaaccgtgc
    20581 gcccgcccga gcgagttctt tttcgatacg ccagccagcg aaaatggctg acaagggaaa
    20641 ccggccagta aaacatcgtg ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca
    20701 tcactcacgc cttctttatg gctgagggtg atgtcgcgga tatcttcatt aaaatgatgc
    20761 gtcgccgggt cgcaataatg attggcttta taagtgcgta ccgcatgttt gttccattcg
    20821 ctggtaaaca cgcactgtcc gccaatcgat tcaaaaccgc gacggatgcc gccaattccg
    20881 gcaaatagat cgataaagcg aaacgcataa tgcggatgat gtgccggtgg tttgggtaac
    20941 aacgtttgca gatgggcgaa ctctttctca cttaaacgat gccatgccga gtcattcgcc
    21001 agcgcacgtt ttaaaattgc cgcgctccag tgattctcac ccaccccatt aagctgcgcc
    21061 accagcgttt taacatcata aatttgcagc agtttctcca gcattgcctg tgcggcattc
    21121 ccggtgctgt atgaatcggt tactgatata ttttcctgca tagatttaac cggccatctg
    21181 agatggccga caggttaaca taattacagc catttagggc cacgcgctta tcccgcgggt
    21241 gaaaacgcat ctatcacctt gtgatcgaca ccttcattct cccccgccag ttcggcactg
    21301 agcttcgcca taaactcgac taaaaaatgc gcgttgtgct gcgccagctg cttgcctctt
    21361 atggtttgca tggtatgcgg tagcttcagc aacttagtct ggaaatgatc cagcgcgtag
    21421 cgtttatcat caagcgggcg atgctgtgcg aacggatctt caccatcaaa cagcgccacg
    21481 cccaatgctc ctgaaacggc aaacacgcgc gccaggccaa tcgcccccag ggcttccagc
    21541 cgatccgcat cctggacaat tttagcttcc gtggttaagg gggcgatttg cgcgctgaaa
    21601 ctgtgagcgg caatggcatg acaaacggcc tcaattttct ccgccggaaa ttgcacgaac
    21661 tcttcacgga gcaggcgacg cgtctcttct gccgccagga ttgaagaacg ctgccgttgc
    21721 ggatgatttt tcgccaggct cacaatatcg tgaaaataac aggcggttaa aatcaccagc
    21781 atatcgacgt catcatctgc cgccagcttc tgtgccgtag cccagacgcg gcgaaaatga
    21841 cacacgtcat gggccgcgtc ctggtgctga tgatggtttt tcaaccagtt ttcgaactct
    21901 gcctgccagt gttgtaagtc catacgcgct ccgttgccaa agagtcgcca ccttagcagt
    21961 ttttattcat agcgtctgct acgtgcggga cgaggtcggg tgtaccaggc aatgctgcct
    22021 aacaatgccc cgaccagagc cataaataaa aatccgacca gtgcccctaa ccactttccc
    22081 gcggtagagc ctaaatcgct ttcaaatccc gacacggggg catcggggat ccggctcatc
    22141 acccagatgt aacgcgccat tgcccagacg aaatagccac accatgtata gaaagcccac
    22201 aaagccagtt tgccaccagg gctgcgagag agcctctctt ccatctttgt cgaaccgtat
    22261 tccattttga gataacccgg aaaattcaga cttagtacta ttatgtgata aaagtcacat
    22321 tttt
//
LOCUS       DAZFRN010000074        22198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFRN010000074 DAZFRN010000000
VERSION     DAZFRN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.074"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            103..345
                     /locus_tag="ACHKUB_002748"
     CDS             103..345
                     /locus_tag="ACHKUB_002748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655735.1"
                     /translation="MMMARDITAYHLNSVHISARRFPEIFQWATGMQTKNLLLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            complement(1271..2791)
                     /locus_tag="ACHKUB_002749"
     CDS             complement(1271..2791)
                     /locus_tag="ACHKUB_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6655736.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DVAALAAARAHSDENDKRTLVSANTYADQKETDINNRTDGLLATERQARTAGDTATLA
                     AARTHSDENNKRTLVSANTYADQKETDINNRTDGLLATERQARTAGDAATLAAARTHS
                     DENNKRTLISANTYADQKETDINNRTDGLLMTERQARIAGDAETLATAEAYSDAGDSL
                     TLKQATAYSDISTRRALSEAYRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPYV
                     AENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVASGW"
     gene            3023..4513
                     /locus_tag="ACHKUB_002750"
     CDS             3023..4513
                     /locus_tag="ACHKUB_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO6655737.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIATALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRREYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNELMHQLAEEQQKTEGLRGTGYDNSGRVQENGHA"
     gene            4506..5987
                     /locus_tag="ACHKUB_002751"
     CDS             4506..5987
                     /locus_tag="ACHKUB_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO6655738.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGRWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESSIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            6055..7092
                     /locus_tag="ACHKUB_002752"
     CDS             6055..7092
                     /locus_tag="ACHKUB_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6655739.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPIRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(7288..7363)
                     /locus_tag="ACHKUB_002753"
     tRNA            complement(7288..7363)
                     /locus_tag="ACHKUB_002753"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7328..7330),aa:Phe,seq:gaa)
     gene            complement(7469..8176)
                     /gene="yqgA"
                     /locus_tag="ACHKUB_002754"
     CDS             complement(7469..8176)
                     /gene="yqgA"
                     /locus_tag="ACHKUB_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO6655740.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            8329..8412
                     /gene="yqgH"
                     /locus_tag="ACHKUB_002755"
     CDS             8329..8412
                     /gene="yqgH"
                     /locus_tag="ACHKUB_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO6655741.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            8574..10709
                     /gene="speC"
                     /locus_tag="ACHKUB_002756"
     CDS             8574..10709
                     /gene="speC"
                     /locus_tag="ACHKUB_002756"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO6655742.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(10759..12015)
                     /gene="nupG"
                     /locus_tag="ACHKUB_002757"
     CDS             complement(10759..12015)
                     /gene="nupG"
                     /locus_tag="ACHKUB_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO6655743.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(12217..13296)
                     /gene="mltC"
                     /locus_tag="ACHKUB_002758"
     CDS             complement(12217..13296)
                     /gene="mltC"
                     /locus_tag="ACHKUB_002758"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO6655744.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(13361..13636)
                     /gene="yggX"
                     /locus_tag="ACHKUB_002759"
     CDS             complement(13361..13636)
                     /gene="yggX"
                     /locus_tag="ACHKUB_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO6655745.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(13664..14716)
                     /gene="mutY"
                     /locus_tag="ACHKUB_002760"
     CDS             complement(13664..14716)
                     /gene="mutY"
                     /locus_tag="ACHKUB_002760"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO6655746.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGRKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            14877..15596
                     /gene="trmB"
                     /locus_tag="ACHKUB_002761"
     CDS             14877..15596
                     /gene="trmB"
                     /locus_tag="ACHKUB_002761"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO6655747.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            15596..15922
                     /gene="yggL"
                     /locus_tag="ACHKUB_002762"
     CDS             15596..15922
                     /gene="yggL"
                     /locus_tag="ACHKUB_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO6655748.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            16106..16825
                     /gene="yggN"
                     /locus_tag="ACHKUB_002763"
     CDS             16106..16825
                     /gene="yggN"
                     /locus_tag="ACHKUB_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO6655749.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            17001..18047
                     /gene="ansB"
                     /locus_tag="ACHKUB_002764"
     CDS             17001..18047
                     /gene="ansB"
                     /locus_tag="ACHKUB_002764"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO6655750.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            18164..19171
                     /gene="yggM"
                     /locus_tag="ACHKUB_002765"
     CDS             18164..19171
                     /gene="yggM"
                     /locus_tag="ACHKUB_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO6655751.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQLVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(19227..20528)
                     /locus_tag="ACHKUB_002766"
     CDS             complement(19227..20528)
                     /locus_tag="ACHKUB_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO6655752.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGIM"
     gene            complement(20528..21031)
                     /locus_tag="ACHKUB_002767"
     CDS             complement(20528..21031)
                     /locus_tag="ACHKUB_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO6655753.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(21076..22062)
                     /locus_tag="ACHKUB_002768"
     CDS             complement(21076..22062)
                     /locus_tag="ACHKUB_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO6655754.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         5670 a         5121 c         5823 g         5584 t
ORIGIN      
        1 agcagtaact ggctatcgtg tataattatc cagaagtcct gctgagcctt ccgtctgttc
       61 atcatgtgcc aggtttgcgt tcgggatatg atgtgccatc ttatgatgat ggcaagagat
      121 attactgcgt accacctcaa ttctgtacac atcagtgccc gccgtttccc cgagattttt
      181 cagtgggcca ccggtatgca aacaaaaaat ctgctgttaa ctaattcttt gttacagaat
      241 gattacgcag aaagtatttt tgtatttaat ggcgaacgtg acgtttcgga aatgccttta
      301 ccggaagaaa tgctactgac tggaaacgct gagtcagaaa gataattcgg tgtattcatg
      361 actgttatca atgtttcatg tgtatgatgt caacgacgga tgaaaagtta tccacttata
      421 tgaggcatta taggcttatt tctggctgac gttgtttcag ttcagaccag gggtagtgaa
      481 tatgctgacc tgctttttcg ctgatatcag tccccctgta attacataga taatagatat
      541 acctctccgt tgaacaggct gtccaggtaa ttgataccgc acttcctcat cagttcgtag
      601 cagagtgtac ttagcatggc tagtgcagtt tctgatgatt tacagtgtgt attcaggaga
      661 gtaagaaatt gttcaaaaca tcctggttta tactggtgct ttgatattca ttgtgtccct
      721 ttttcgttcg ttattatttc catatttaat atgatgcctg tacatgtaaa ataatggaag
      781 atttcagggg agcattgcat aaagtccgcc tggagaataa ggctcaggtc agcgaatcgc
      841 atcgtggcca gactgcactg actgcaataa gggcggatac agagttgcag ctactcgtcg
      901 atttcagaac aacagaatcc ttgcaaacag ggatgtgttc attcaggggt tatgatgcta
      961 cgacgaacgc ttttagtgat tattaatcga cgatactgtg ttttgtctgg atggtgaggg
     1021 actcgctttg aggattcacc agggaaatac ggggcttgtg gcaataatgt tagcctgcga
     1081 acttaaatgt ttacgggcaa catggcggca ggaaatctgc cgccggattg tagtgaaggc
     1141 tgaccacact gcgcgccctt tttccttgat tatcccgctc agtccagtgg tctgttccgg
     1201 atggggctgt gttgcgtact gaattaatgt gattacggaa taccataatg ccgtatgtac
     1261 gcaagattat ttaccagccg ctggcaaccc ctgcaccaat cgcggtgtta tgagatgagt
     1321 cccaggagat gttgaggcgt atattggtat tacgtgatgt tttatactga atgcctgcgg
     1381 ccacagcatt agcgttctga tagttaccca cgccgatccc gtatgaaaaa gaattttcag
     1441 ccacataggg gatggaggat attgctgtca ctccggcgat gcctgcatta agtctttttt
     1501 ctgcccgttc aattttattg ctcaggtttc tgaatttgtt gtcggtataa cgataggcct
     1561 ctgacagtgc ccggcgggta ctgatatcgc tgtaggctgt tgcctgtttc agtgtcagtg
     1621 aatccccggc gtcactgtat gcctctgctg ttgccagagt ttctgcatca cctgcgatgc
     1681 gtgcctggcg ttcggtcatc agtaatccgt cagtacggtt attgatgtcg gtttctttct
     1741 ggtcagcata ggtattcgca ctgatcagtg ttcttttgtt gttttcatcg ctgtgcgttc
     1801 tggcagcagc cagagttgca gcatcgcctg cggtgcgtgc ctggcgttct gtagccagta
     1861 atccgtcagt acggttattg atgtcggttt ctttctggtc agcataagta ttcgcactga
     1921 ccagtgttct tttgttgttt tcatcgctgt gcgttctggc agcagccaga gttgcagtat
     1981 cgcctgcggt gcgtgcctgg cgttctgtag ccagtaatcc gtcagtacgg ttattgatgt
     2041 cggtttcttt ctggtcagca taagtattcg cactgaccag tgttcttttg tcgttttcat
     2101 cgctgtgggc tctggctgca gccagcgctg ctacatcacc ttctgttcgg gccctggttt
     2161 cagttatgac ctgaccagag agggtattaa gctgattctg cagattgttt gttgatttta
     2221 ttctttctcc agcttcccgg ttaacagcac tggtcatctg catcagattt accgcatcat
     2281 ggtcatcttt cccctgtgcc agatttgtga tgcggcgttt cagtgtgtca gagccgacgg
     2341 atacgatatt gtttccgtta gcactggaat gactgcccag tgccacggag tcgttcccgg
     2401 ttacggacga tgtcagacct atggcaacag atgaatcacc gttaattctg gtggcattac
     2461 cgaggctgac gctgtaacca tccagcacaa tatcttcgcc cacttttctg atcaaaccgg
     2521 aagctttgac cgtggaggaa ttaccaataa caatgttgtt ataccctccc tccacggaat
     2581 tactgttacc cacaaccaga ctgtttgact ctgatgtgac agtattttca ctgccgtaaa
     2641 tatttccgga gaatcgtgtt gttgtattgt ttttttctcc ggtgatgttc ggtgtgttga
     2701 catcagcatg tgctgtctga cagacgatgg cggctgacag catagctgca actgttaatg
     2761 aataagaaac gtgttttttt acttcggaca ttattttttc ctgccagatt atgacctttc
     2821 aggtcccggt atcctggata agcgcggcag acaggagacc acctgccttt ctgaggacag
     2881 gatgagaagc atccggtgtg atggcgatga ttaattcatc atatgaccag ggaaattttt
     2941 ctgttgctgt acagagttat ttgtggtggg catacccctg atgcatcaga ttacggaaat
     3001 gtttttccgg agaagagaat gcatgtcttt taatcccaca gatgaacagt acgccattat
     3061 taactggcag gggcagcaac tggttgtgaa tgcgttttcc ggcacaggga agacgagtac
     3121 gctggtgcag tttgcccggg ccagtccgga cagccgtatg ctctatcttg cctataaccg
     3181 ggcaatacgt gatgaggcag agcgtaaatt tccctttaac gtggaatgca gaacgtcaca
     3241 tcagctggcg tatgcccggg ttggaagaca ctaccgtcat cggctggttc cgggattacg
     3301 tattactgat gtggcgagaa agctgaatac acgatactgg gctctggcac gggtggcagg
     3361 gacggggctg aatcacttta tctgttctgc cgatgcggta ccgggcctgc atcacctgcc
     3421 ggataaggat gagatgcggg gtgttccgcc agcagatgcg cttcgggcgg tgcagttgct
     3481 ctggaacgaa atgagcaacc ccggggggac gttcccggtg acgcacgata cttatctgaa
     3541 actcttccag ttgtccggtg cagacctgtc tcaccgatgg gacaccatcc tgtttgatga
     3601 ggcgcaggat gcgaatccgg tgaccactgc tctggttctg gggcagaagt gtcgggtggt
     3661 ccttgttgga gattgttatc aacagattta ccgctttcgg ggtgcgaata atgcgctttc
     3721 ccatcctgca ctgaagaatg cggaccggtt gtggctgaca cagagtttcc gttttggtcc
     3781 ggcggtggct cgtatggcaa atctgcttct gcagcgtgaa ggtgaaacac gagaggtgaa
     3841 aggttgtggt ggtgatgatg aggtattgct gaaatgccat gcgcgtgaac atcttcaggg
     3901 gcattacacg gtactcagtc gtactgtcgc gggtgtgatt gctactgcgc tgatggcggc
     3961 aatgaaaggg caaaaggttt actgggtggg gggaatcgaa ggttacagga ccggggaact
     4021 tgaggatctc tactggttcc aggttgatat gccggaacgg atgcattcag acaggctgcg
     4081 ccgggagtac aggaattttg aagaatataa atatattgca aaatccacga aggatgtgga
     4141 gatgaatcag tcccttcggc ttctggagtt gtgttttccg ttaccgaaaa aactggaact
     4201 tctccggcag tatacggtaa cgaatgagca ggatgcagat atcacggtgt caacggcaca
     4261 ccggagcaag gggctggagt ggccggttgt aatgctggat gaggatttcc aggatatcac
     4321 ggatccgctg atgacggagg ctgagcgtca tgatgaaacc tgtctgttat atgtggctgt
     4381 cacccgggcc ctcaggacgc tggtactgaa tgagctgatg catcaactgg cagaagaaca
     4441 gcagaaaact gaaggtctgc gggggaccgg gtacgacaat tcaggtcggg ttcaggagaa
     4501 tgggcatgct tgaacgcctg aaaaggttat ttcctgtgaa gacagaatgt tctgatgtcc
     4561 ggacggatat tcccggggaa acggtgccgg aggggtattt tacaccattg ccggctgatg
     4621 tactggtatc cacaccgttc aggaaaaggt gtctgaaaca gttatgggaa aacagttcca
     4681 tgccggtgga tatgtatcag cgcctttgtc ttgatcctgt gcatcagttg ttgtctgtca
     4741 gtcagaatct gccggccaca gcagaaggtc gctggggtta caccggtggt tttggtgacc
     4801 tgaccctgca gttcacgacg tatgctgtcc ggctggccag aggcagaatg tttccaccgg
     4861 gagcgacagc agaagagcag gccgcgcagg ggagtgtgtg gaacatggta gtgttctggt
     4921 cggctctgtt ttatcacctg ccgttgctgg cggaactgga aggcgaacag gccgacggca
     4981 gatgctggca gccggggata tctgtaccgg agggcgtata ccggttccgg ttcaggaaga
     5041 tgccctgcca gggaacggag ggacggcaac tggcagccct gatggcggga caactgctgc
     5101 ccggagaggc gggagcctgg cttgccactg tcccgggggc attacagaat cttgccggag
     5161 cagtctggca tcagcatcct gaaatggcgc tgatacgtac agtattacag gaagctatgg
     5221 aggaagtcgt gagtcccctc gctgcaggag atgtggccga agttccggtg gctgaggttg
     5281 ttccggtact gctgcagtca gcagcaattt cagcagaaag cgttattcag ccgttgccgg
     5341 ttgtacagtc gccagtggag gatgccgaaa atgagggccg gacagcggaa caggaggtgg
     5401 atgaggggaa cgaaactgat gctgataccg gaatgctcct tacgctgttt tcggctgctg
     5461 atttgtcgga tgagggaggg cagatagcgt caggcgtgcc gccggataaa gaaagcagta
     5521 ttgagttacc ggaagagagc ggatgtgtaa cagaaaatca gggaaaccgt atcccggaaa
     5581 cagtggaaaa aacggcagag ttttccctga cagaggatag cagggtagag agtgaattta
     5641 tcagatggtt aaaagagggg ctgagggagg gttcactttc ggttaacagc gttgattcaa
     5701 atgttcatgt tgtttccgga tatgtttttc tgtgtgcccc ggggattttt tatcgttttc
     5761 tgaaggagag tggttccggg caggatcgtc gttatgttca gtcgctcttt gaacgggcag
     5821 gtatccataa ggtacgttca ggagagcgtt tttttcaggc ccgtctttat agtgacgcag
     5881 aacattccgg acgttacaaa atggtttcgg gatatctggt taagggcagg tttctgtacg
     5941 ggaatattac ggttcccgga gacagcccgc ttattgtttt cgggtgagta aaggcgatga
     6001 tgtgcagaat ttgaccggag agtgcgggag cacagtaaac aaggggaata tataatgcag
     6061 aatgacgatt ccgtcacaaa aatcacctgg gaacaactgc tggaagagta ctttttttca
     6121 aagaatcttc ggccggatac agagtggagc tactgcaaag ttgtcagggt attcagggaa
     6181 tttatcggtg atattttgcc gggggaggtg actgtccgtg atgttcagcg ataccggcgt
     6241 tatgtcctga aagagcagaa gcgcagggag gtgacctgga ataacaaggt ggcccacttg
     6301 cgggcaatta tgggattcgg gatcaagatg aaactgctgc ctcagacaga aaacccgttg
     6361 gtggaagcat cagttgcgag tactgaaaaa aagaaaaaga cactgagtga caatcagatg
     6421 ctcagggttt acctggttgt gcagaggttt gcagaagcgg aagagcagaa catggtgaat
     6481 agtcgtcgac gcaatgctat ataccctgcc tggtactggc tgacagttct ggatgttctg
     6541 cgttataccg gaatgcgctt taatcaactg aagcatatcc gtgtgaggga tgtctgtccg
     6601 gatgaaagca taattgatct taagctggag ggaagtaaaa ctcacaggga atggggcgtt
     6661 cctctggtgg ctcccctgaa ggtatcgatg gaaaaactgc tggagcgggc ccgtcagtgc
     6721 ggggcaggtg atgatgacta tctttttgac gtctgtcgtt tcagtgagcc gatctctgcc
     6781 gacagatatc agtactgtcc cattcgggcg catcagaccg tacgttcatt tttccggcga
     6841 ctttcgcgag aatgtggttt tcctgtttca ccacatcgtt tcagacatac gctggcaacc
     6901 agactgatga aatatcctga acgtaattta catctggtca aatccatact gggacacagg
     6961 agtcttacaa cgaccatggg gtatgttgat gtctgtatgg agtctgcagc gcagacactg
     7021 gaagatgccc tgagtttgta tacagatgag cgcacagtga gcaagatttg caaagggggg
     7081 aataaatctt gacaggctga atcagcgatg taagcataca gtgttaacga gaacagggcg
     7141 tctccggaaa agacaccctg tttgaggata cgataccaaa ctcaatcagt gtaaccacaa
     7201 cttacacaaa cggagaccct ttgatgccgc atcctgtatt aaccgcccgt caaggatctc
     7261 atcccttaac cagcgtgttc tggtatatgg tgcccggagg cggaatcgaa ccaccgacac
     7321 ggggattttc aatcccctgc tctaccgact gagctatccg ggcaacgggg cgcattaaac
     7381 ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta ctgcagactg cacaatctat
     7441 catcactttg ccgctattgc acgcattgtc aggcaaacca ggcggtccag gcggcggaaa
     7501 gcggcattgc cagaaggagt gctggaagca tgttgaccac cgggaacatt ttaatgccac
     7561 agatgcgtaa tccggttgcc agcagcaata aaccgcctac ggcgctgaag tctgccatca
     7621 tcgacggtgt ggtcagcggt aatatcagcg cggcagccca cgccagcgtt aactggatga
     7681 tcagtaatgg gatactaatt accgacaccg caatacccag cgagcaggcg aagatcatcg
     7741 ccgtaaagaa atcaagaaat gacttggcga ttaaaatact cggatcgccg gtcatccctt
     7801 cgttcatcgc cccgaagatc ccggtgccgc tggcgcaaaa caggacaata atcgcgacat
     7861 aattctgaat aaaagattca tgcgctggct tcttacgtga gtgacgaaac agattttgtg
     7921 ctttggcgac tgctgtattg acacctttct ccagcaaaca aatttcgcca attagagcgc
     7981 caagtagcgt agctaaaacc atcgctggaa ggttggcgca tttcacaacc agtaaaatac
     8041 caatccccag cgatgccaga ccaaaaatag atgtcatgga gacgcggata cgttccggta
     8101 agcgttggct gagcagtgcg ccgagaacgc cacccagtaa gactgcacta gcgttgataa
     8161 aagggccgat gaccacaaga gttcctgtta gctgtattgt ttgattttct tattatgaac
     8221 tttttgcctt gttgatggct tgtctggtcg caaagtcctg gcttgcacgc tttagcgaaa
     8281 ggtgccatga ttgcgcgaat tttctcctct ctgtacggag tttgcccgat gcacgccatc
     8341 tccttacatt ctctcgctta tcgccgtttc gcgcgaaacg tttccctttc tatgttactg
     8401 ctcatgcggt gaagttaacg cacgctcact gcaggacaac agtaaaatca gagcgtttct
     8461 gcttttactg atgtctggcg gtcggagctg gtgaccagtt tgacccatat ctcatggggc
     8521 agggttttcc accttgtccg gtattcttac ttccccgaaa cgggtttgcg cttatgaaat
     8581 caatgaatat tgccgccagt agtgaactgg tatcccgact ttcttctcat cgtcgcgtgg
     8641 tggcgttggg agatactgat tttacggacg tcgcggcagt cgtcattacc gctgcggata
     8701 gtcgcagtgg cattcttgcg ttgcttaagc gcaccggttt tcatctaccg gtgtttttgt
     8761 attccgaaca tgctgttgaa ttacctgcgg gcgttacggc ggtaatcaac ggcaacgagc
     8821 agcagtggct ggagctggaa tccgcagcct gtcagtatga agagaatttg ctgccaccgt
     8881 tttatgacac gctgacgcag tacgttgaga tgggcaacag cacctttgct tgccctggac
     8941 atcaacatgg tgcgtttttt aaaaagcatc ctgccggacg ccatttttac gatttctttg
     9001 gtgagaacgt ctttcgcgcc gatatgtgta acgctgacgt aaaattgggc gatctgctta
     9061 ttcatgaagg atcggcgaaa gatgcgcaga aattcgcagc caaagtcttt catgccgata
     9121 aaacctattt tgtgctgaac ggcacatcgg cagcgaataa agtggtgacg aatgcgctgt
     9181 taacgcgtgg cgatctggtg ctcttcgacc gtaacaacca taagtcgaat catcacggcg
     9241 cgttgattca ggcgggggcg acgccggtct atctggaagc ttcacgtaac ccgtttggtt
     9301 tcattggcgg tattgatgcg cactgtttta atgaagagta tctgcgccag caaattcgcg
     9361 acgttgcgcc agaaaaagcc gacctgccgc gcccgtttcg cctggcgatt atccagctgg
     9421 gaacctatga cggcactgtc tacaacgccc gtcaggtgat cgataccgtt gggcatctgt
     9481 gtgattacat tctgtttgat tccgcgtggg tcggttatga acaatttatc ccgatgatgg
     9541 cggatagctc gccgctgcta ttagaactta acgaaaacga tccggggatc tttgtgactc
     9601 agtcggtgca caaacagcag gctggattct cacagacgtc gcagatccat aaaaaagata
     9661 accatatccg cggacaggcg cgtttttgcc cgcataagcg gttgaataac gcctttatgc
     9721 tccatgcttc taccagccct ttctatccgc tgtttgctgc actggatgtt aacgccaaaa
     9781 ttcatgaagg ggagagtggg cgtcggctgt gggctgagtg cgttgagttg ggtattgagt
     9841 cgcgcaaggc tattcttgcg cgctgtaagc tgttccgccc gtttatcccg cccgttgttg
     9901 atggcaaatt gtggcaggat tatccgacat ctgtgttagc cagcgaccgc cgttttttca
     9961 gttttgagcc gggggcgaag tggcacggct ttgaaggata tgccgcggat cagtattttg
    10021 ttgatccgtg caagctgtta ctcactacac caggtatcaa tgccgaaacc ggcgaatata
    10081 gcgactttgg cgttccggcg acgattctgg cgcactatct gcgtgagaac ggcattgtgc
    10141 cggagaagtg cgatctcaac tccattctgt tcttattaac tccggcggaa agccacgaga
    10201 agctggcaca actggtagag atgttggcgc agtttgaaca gcatattgag gatgactcgc
    10261 cgctggctga ggtgttgccg agcgtttata acaaatatcc ggtgcgctat cgcgactaca
    10321 ccctgcgcca gttgtgtcag gagatgcacg atctgtatgt cagtttcgac gtcaaagacc
    10381 tacaaaaagc gatgttccgc cagcagagtt tcccgtcagt ggtgatgaac ccccaggatg
    10441 cgcatagcgc ttatattcgc ggtgaagtgg agttggtgcg gattcgtgat gccgaagggc
    10501 gaattgcggc agaaggggcg ttgccttatc cacctggcgt gctttgcgtg gtacccgggg
    10561 aagtctgggg tggggcggtt caacgttatt tccttgcact ggaagaaggg gtgaatttgt
    10621 tgccgggatt ttcgccggag ctgcaaggtg tttatagcga aaccgatgcg gatggcatga
    10681 aacggttgta cggttatgtg ttgaagtaag aataaaaaaa acgggtcacc ttctggcgac
    10741 ccgtttttct ttgcgtaatt agtggctaac cgtctgtgtg cctgtcggga cacgaacgtg
    10801 tttatatttg aacatcgcca tgaacgcgaa ggccagaacc acggagtaac cagcgaaaat
    10861 caaccatacg gtctgccagt cggtaatgcc gttttgggtg tacatctcaa caactttacc
    10921 gctcacgatg ccgccgagga tacagccgaa gccgttagtc atcatcagga acatcccttg
    10981 tgcactggcg cgaattgccg ggctaacttc tttttcgaca aacaccgaac cagagatgtt
    11041 gaagaagtcg aatgcgcaac cgtaaacgat catcgacagt accagcagta cagtaccgaa
    11101 cggagtcggg tcgccgtaag caaacagcgc aaaacgcagg atccacgcca caatactgat
    11161 catcattacg ttcttaatac cgtagcggct taagaagaac gggatggtta gaatgaacag
    11221 ggtttcagag atctgcgaaa tcgacatgat gattgacgca tgctgcacaa taaagctgct
    11281 ggcaaacatc ggatctttgt cgaagctgtg caggaaggta ttaccgaaca tgttggtaat
    11341 ctgcagttcc gcgcccagca gcattgagaa gataaagaag attgccatac gcttgttttt
    11401 aaacagcgcg aatgcatcga ggcccagcag ggttgtccag ctctgattcg cttgctgttt
    11461 agcaaccgga atatgcggca gagtcagggt aaacagaacc agaatggcgg aaagtgctgc
    11521 gccaatatac agctgcatgt ggcttaattc gaagccagac aggctcacca cccacattgc
    11581 catgataaag ccgatggtgc cccagatacg gattggcggg aagtcagtaa cgatatccat
    11641 cccggcattt tgcaggcgat agtaagagat ggtgttgatt aacccaagcg ttggcatata
    11701 agcaaacgag ttaatcaata tcacaaggaa catcgcctcc ggtgtcgtga cctgtgccgc
    11761 catgaacagc gtgatagcgc caatggtgtg gcaaatggca tatacccatt tcgcacttaa
    11821 ccatttgtcg gccacaatcc ccagcagcgc aggcataaag accgcggcga tacccagtga
    11881 gctataaact gcgccaatag aagcaccgtc aaacttcagg gtaacaaaca tataggagcc
    11941 gagggtcgtc agccaacttc cccacagaca gaactgcaga aaagagagga ttttcagctg
    12001 cagcttaaga ttcatgttaa tttcctcaca tcgtgatgcg gatgagtgtt tgaaaaagca
    12061 catttgaccg tgcgagatga tgggattcta tcaacttcgc ggactggttt tttgttacct
    12121 gtggcaaata attgcaaaca ttcccaattt gcaaaacgaa attgtgatgt ggataacatt
    12181 tttgcccctg agcatcgtca ggggcggtta atggaattat cggcggcggt aggatttttg
    12241 cgcggtattc actttataaa gataacggcg agattccgca gagggatggc gggtcgtcag
    12301 cgtctgataa acatcgcccg gcgtcatggt gttaataata ttggcagcct gaatcttatc
    12361 attcgaaaag actcgcagca cgctgcctgc gccgccgtta taggcggtga tgacggcata
    12421 acgccgcgat gttgggttat caattccgcc gagataaaca ttgttcagca tcgccagata
    12481 cgcggtgccg gtatcaatat tgctggcagg atcaaacaag aaactgcggc tcggcgtgcc
    12541 ggatttcccc tgcgagcgga acacatcttt cccggcagta tgttgtacca cctgcattaa
    12601 tcccagcgca tcggaacggc tgaccgcata cgggttaaag gaagattcgg tctgcataat
    12661 tgccagaatc agcgactcat caacgccata tttccgtgac gcctggcgga ccatgccgag
    12721 atatttgtgc gcacgtttat caaggtggtt cggtaccatg ttaatggtga cgctgtagat
    12781 gatacgcagc ccgttactgc ggctttgcag acggtttttc agcagataat ccgcgaagtt
    12841 gcttgcgcga ccttcccagc gaatcggctg cccggtgttg tccaccacct gaccgtaaag
    12901 gaaaggttct ttcgaaatcg taatatcatc aacgtcggaa tagagatcga ccgaactcgg
    12961 atcgtcaccc atcaataacg ttttgataat tgcccggcgc aaatgcgcgg caggttctgt
    13021 cccggcgatg gtttcgatag taatcgtacc gtcatcgaag ttgatgtggc tgcgggtctg
    13081 atattgatcg gtgtatttca cgtagtcctt aggaccagcg atcaccacct ctttgaatcc
    13141 ccagatgttc tcaatattgt gggcaaattg ccccatcaga atatcaaaac cgttggtgtc
    13201 tttgacccag gcttcgttat aggtatcgcc ttttttggtc gtcgaacagg agatgagcaa
    13261 cggcgcaatc aaagccagcg cgagatattt tttcatcatt ccgggtgcgt gttgtgtttg
    13321 tttttatgca agttgccgga ggcgtgctcc ggcactgttt ttatttttta tcttccggcg
    13381 tatagccctc gatatgcacc tctttaccct cgaacaggaa gttgaccatc tcctgctcaa
    13441 gcagcttgcg gtgctcggca ttcatcatgt tgagtttctt ttcattaatc agcatggttt
    13501 gcttgtgctg ccactgcgcc caggcttctt tggagatctc gttatagatg cgttttccca
    13561 gctcgccggg gtacagctga aaatcctgac cttctgcttc acgttgcagg aaagtacaaa
    13621 aaatcgttct gctcataaat catcctcttt atcgactcac gcgctaaacc ggcgcgccag
    13681 tgcgtaactg ctgtaacaaa cgctccacgg gagccgccag gccaactgac ggcggttgcg
    13741 ctaagttata ccagagcgca ttgccttcat ccatgcagcc ggtgaatgac gacacgggaa
    13801 gccacatagg cacaatatct aagtggaaat ggctgaaggt atggcgaaac gcggtcagct
    13861 gcgtcaggtt atcggcagca atctgccgtt gcgccagcca atgccgcaaa ctttcttcgt
    13921 cggcaaactg cgggaaacag tataaaccgc cccacaatcc gctcggcgga cgctgcgcca
    13981 gcaatacttc atcttcgtgc tgtaatagca aaaagtagcc ggtgcgctcc ggcagcgtct
    14041 gtttcggttt tctgcccgga taaagcgacc agctattgtt ggcggcggca atacatccgt
    14101 tttgtagcgg acagagcgaa catttcggtt tcgagcgcgt acaaatcatc gcgcccaaat
    14161 ccatcatcgc ctgattaaac cgttccacgc cgaccgcggg cgtcacctgc tcgcttaaac
    14221 tccataattt attctcgacc tcttttttcc caggccagcc gcttacagca tagcagcgcg
    14281 ccagcacgcg tttgacgtta ccgtcgagaa tcggaaagtg cttacccaga gaaagcgaga
    14341 gaatcgcgcc tgcggtggaa cgcccgacgc ccggcagtgc tgcaacttcc tcaaaggttt
    14401 ccgggaattt accgccgtgt aaggtcgcca cttgttgtgc cgctttatgc agattgcgcg
    14461 cgcgggcgta atagccaagc ccggtccaca agtggagaac ttcgtcgagc ggcgcattgg
    14521 cgagatcggt caccgtcggg aagcgcgcca taaagcgttc aaaatagggg ataacggtcg
    14581 caacctgagt ttgttgcaac atcacttctg agagccatac tttgtagggc gtcttgtcaa
    14641 tttgccaggg cagagttttt cgcccgtatt tatcgtacca gtccagaacc tgggctgaaa
    14701 attgcgacgc ttgcatggtc accgaattca ctgttgttgg gggcaagatt gcagcacagc
    14761 gacggcaggg tgtaaaccgg aactttccgc agccacggcc ttatcatgct tgcatccggc
    14821 aactaacttt ggataatgcc cgttttcaga acactttcac aagcgactaa atctttatga
    14881 aaaacgacgt catttcaccg gaatttgatg aaaacggccg cccactgcgc cgtatccgta
    14941 gttttgtgcg ccgccagggg cgactgacca aaggccagga acatgcgctg gaaaactact
    15001 ggccggtgat gggcgttgag ttcagcgaag atatgctgga tttccccgcg ctttttggcc
    15061 gtgaagcgcc ggtgacgctt gagattggtt ttggcatggg ggcgtcgctg gtggcaatgg
    15121 ctaaagatcg ccctgagcag gacttcctcg gcattgaagt gcattcaccg ggcgttggtg
    15181 cgtgcctggc ttctgcgcat gaagaaggtt taagcaacct gcgcgtgatg tgtcacgatg
    15241 cggttgaagt gctgcataaa atgattcctg acaattcatt gcgcatggtg cagctctttt
    15301 tccctgaccc gtggcacaaa gcgcgccata ataaacgccg tatcgtacag gtgccgtttg
    15361 ccgaactggt aaaaagcaaa ctgcagctgg ggggcgtatt ccatatggcg accgactggg
    15421 aaccttatgc ggaacatatg cttgaagtga tgtcttctat tgacggttat aaaaacctgt
    15481 cagagagcaa tgattacgta ccgcgtccgg catcacgtcc ggtgacgaaa tttgaacaac
    15541 gtggtcatcg tcttggtcac ggagtatggg acttaatgtt cgagagggtg aaataatggc
    15601 aaagaaccgt agccgtcgtc tgcgtaaaaa aatgcacatc gacgaattcc aggaattagg
    15661 attttcggtg gcatggcgat tcccggaagg tacatcggaa gaacagattg ataaaaccgt
    15721 tgatgatttt attaacgagg ttatcgaacc gaacaaactg gcctttgacg gcagcggtta
    15781 tctggcctgg gaaggtctga tctgcatgca ggaaatcggc aaatgcaccg aagaacatca
    15841 ggcgattgtg cgtaagtggc tggaagagcg caaactggat gaggtacgca ccagcgaact
    15901 tttcgacgtt tggtgggact aagaaagcat acgggcgatg acaaatgcaa aactgcctga
    15961 tgcgctacgc ttatcaggcc tggaaagatg cacgatcgag taggcgggat aaggtgttta
    16021 cgccgcatcc ggcatggaaa acgcgtactt tgttatcaat ctggggccag caaatgctgg
    16081 cctgattttt tcttgaggga agactatgat gcgcaaaatg ctgctggcgg cagcactttc
    16141 agtgacggca atgaccgctc acgccgacta ccagtgcagc gtcacgccgc gtgacgatgt
    16201 gattgtcagc ccgcaaaccg tgcaggtgaa gggcgaaaac ggcaatctgg tgatcacgcc
    16261 agacggcaac gtgatgtata acggtaagca atattccctg aatgccgccc agcgcgagca
    16321 ggcgaaggat tatcaggctg aactacgcag cacgctgccg tggattgatg aaggcgcgaa
    16381 aagccgcgtc gaaaaagccc gtattgcgct ggataaaatt atcgttcagg agatgggcga
    16441 aagcagcaaa atgcgcagcc gtctgaccaa acttgatgcg cagctgaaag agcagatgaa
    16501 ccgcattatc gaaacgcgca gcgatggcct gacgtttcac tataaagcca ttgatcaggt
    16561 tcgcgccgaa ggccagcaat tagtgaatca ggcaatgggt ggaattttac aggacagcat
    16621 taatgaaatg ggcgcgaaag cggtgctgaa aagcggcggt aacccattac agaatgtgct
    16681 gggaagcctg ggggggctgc aatcctcaat ccaaaccgag tggaaaaagc aggaaaaaga
    16741 tttccagcag tttggcaaag atgtttgtag ccgcgttgtg actctggaag atagccgcaa
    16801 agccctggtc gggaatttaa aataatcctc tattttaaga cggcataata cttttttatg
    16861 ccgtttaatt cttcgttttg ttacctgcct ctaactttgt agatctccaa aatatattca
    16921 cgttgtaaat tgtttaacgt caaatttccc atacagagct aagggataat gcgtagcgtt
    16981 cacgtaactg gaggaatgaa atggagtttt tcaaaaagac ggcacttgcc gcactggtta
    17041 tgggttttag tggtgcagca ttggcattac ccaatatcac cattttagca accggcggga
    17101 ccattgccgg tggtggtgac tccgcaacca aatctaacta cacagcgggt aaagttggcg
    17161 tagaaaatct ggttaatgcg gtgccgcaac tgaaggacat tgcgaacgtt aaaggcgagc
    17221 aggtagtgaa tatcggctcc caggacatga acgataatgt ctggctgaca ctggcgaaaa
    17281 aaattaacac cgactgcgat aagaccgacg gcttcgtcat tacccacggt accgacacga
    17341 tggaagaaac tgcttacttc ctcgacctga cggtgaaatg cgacaaaccg gtggtgatgg
    17401 tcggcgcaat gcgtccgtcc acgtctatga gcgcagacgg tccattcaac ctgtataacg
    17461 cggtagtgac cgcagctgat aaagcctccg ccaaccgtgg cgtgctggta gtgatgaatg
    17521 acaccgtgct tgatggccgt gacgtcacca aaaccaacac caccgacgta gcgaccttca
    17581 agtctgttaa ctacggtcct ctgggttaca ttcacaacgg taagattgac taccagcgta
    17641 ccccggcacg taagcatacc agcgacacgc cattcgatgt ctctaagctg aatgaactgc
    17701 cgaaagtcgg cattgtttat aactacgcta acgcatccga tcttccggct aaagcactgg
    17761 tagatgcggg ctatgatggc atcgttagcg ctggtgtggg taacggcaac ctgtataaat
    17821 ctgtgttcga cacgctggcg accgccgcga aaaacggtac tgcagtcgtg cgttcttccc
    17881 gcgtaccgac gggcgctacc actcaggatg ccgaagtgga tgatgcgaaa tacggcttcg
    17941 tcgcctctgg cacgctgaac ccgcaaaaag cgcgcgttct gctgcaactg gctctgacgc
    18001 aaaccaaaga tccgcagcag atccagcaga tcttcaatca gtactaatca cctcgccccg
    18061 gtatcgtgcc ggggcttttt cacttcagac ttacgtccat tgccaatttt cattacccta
    18121 atgataatca ccggaataaa ttattccgcg tgaggttttt cgggtgaaaa agcaatggat
    18181 tgttggcacg gcactgctta tgttgatgac tggtaatgcc tgggcagatg gcgaaccgcc
    18241 aactgaaaat atcttaaaag atcaattcaa aaagcagtat cacggcattc tcaagcttga
    18301 tgctatcacc ttaaaaaatc ttgatgctaa gggtaatcag gccacctggt cagcggaagg
    18361 cgatgtctct tccagtgacg atctctatac ctgggtcggt cagttggcag attacgaact
    18421 gctcgaacag acctggacga aagataaacc ggtaaaattc tcggcgatgt taaccagtaa
    18481 aggaacgccc gcgtctggct ggtcggtgaa cttttactct tttcaggcgg cagccagcga
    18541 tcgtgggcgg gtggtcgacg atatcaaaac gaataataaa tatctgatcg tgaatagcga
    18601 agatttcaat tatcgcttta gtcagcttga atctgcgttg aatacccaga agaactcgat
    18661 tcctgcgctg gaaaaggaag tgaaagcgct tgataagcaa ttggtggcgg cgcagaaagc
    18721 agcggatgca tattggggaa aagatgcaaa cggtaagcaa atgacccgcg aagaagcctt
    18781 taaaaaaatt caccaacagc gtgatgagtt taataaacag aacgatagcg aggcgttcgc
    18841 ggttaaatat gacaaagagg tttatcaacc ggcgattgcg gcatgtcata aacagagtga
    18901 agagtgttat gaagtgccga ttcagcagaa acgagatttc gatatcaacg aacaacggcg
    18961 acagactttt ctgcaatcac aaaaactcag ccgtaaattg caggatgact gggtaacgct
    19021 tgaaaaaggg caatatccgt taacgatgaa agtttcagaa ataaacagta aaaaagtcgc
    19081 gattttgatg aaaattgacg atatcaacca agctaatgaa cgctggaaaa aagataccga
    19141 acaactgcgg cgcaatgggg tgattaagta agtttgatat agtgcctttg ccggataata
    19201 taaaaagtta tccggcaaat tattttttac attatcccta gtagctgtgg aatgaacaag
    19261 gatatttgcg gaatgtaagt gatcatcatc aacgctacta gcaatgcagc ataaaatggc
    19321 agcaatggtt tgattaaatg ttgaatcttc acacccgaga tagaacaacc aacaaatagc
    19381 gcactaccca ccggcggtgt taacagccca atacaaaggt tggccaccat cataataccg
    19441 aaatgtactg ggtccattcc cagctcttgt gcaatcggca agaagatcgg tgtaaagatt
    19501 agtacagccg gtgtcatgtc cataaagatg ccgacaatta ataagacgat attaatcagc
    19561 agcaatatga tcagtgggtt atcagaaatc cccatcaatg cgtcgctgat catataagga
    19621 atatcagcat tagtcattgc ccaggacatg cccacggaaa aaccgatcaa cagtaataca
    19681 atcgaagtgg taacaaccga ctccagaatc agtttgggta aatggcgcca ttttacttcc
    19741 cggtaaacca gcaccgagag aataaatgtg tagacaacgg caattgctga cgcttcagtt
    19801 gcggtaaaaa tgccgcccag aatgccgccc atcacaatga acaccagaag caaacttggt
    19861 aaggcatcca gaaacgcttt acaggcctga gcaaaggttg ctctttctga aagcgggtag
    19921 ccacggcgtt tagcaataat gccgcagact atcattaccg caagacccat taagatccct
    19981 ggcaaatatc ccgccataaa tagcgatgct acagaaacgc cgccagctgt cagggaaaac
    20041 acaattaaca cgtttgaggg cggtatcagt aatccagtaa tacaagatga gacgtttaca
    20101 gcggtagaaa atgccggatc ataaccttgt ttggtctgaa tgggattcag tgtgccacca
    20161 acggcagcag ctgccgcaac ggctgaacca gaaatggaac caaacatcat attcgccagg
    20221 acgttcacat gaccaagtga accaggaacc cgaccaacca ttacctgggc gaggttaatc
    20281 aaacgtatcg caataccgcc gctgttcatc agcgttccgg caaagatgaa aaaggggatt
    20341 gccaataggg caaagttatc cagtccatta gccagtcgct gcgaaacagt gattgccgcg
    20401 atatcccagg gaaacatcaa aagcattgaa gctacagtgg caataccgat tgagaacgaa
    20461 atgggcacac caataaagac caacacaaaa aaacagccaa acagtgtaat cgcgatataa
    20521 ctatccatta actggcctcc tgcgcatgtt gtttcagttg ttgaatgctt tgattaataa
    20581 aacacaaggc ataaaacatc atcaccaatc cacttaatgg cagaatgata tatacgtatc
    20641 ccatcggtat ttgcatcgcg gcagatacct gtgatgttgc cagcgtttta tcaattaatt
    20701 tcaggccacc agtcacaatg accgaaccgg caaaacctaa aataagaacg ttaatgacta
    20761 tactaagaag taattgcttg cgcttattca gggcgagagc aaataaatcg atagagagat
    20821 gtctctgcgc gccgacggta tatgcagcac ctaataaccc tacccagatc atcaaaaatc
    20881 gtgccagctc atcggttaat gtacttggct ggttaagtac gtagcggcta aatacttgcc
    20941 agactacgca tacaaccaga gcaagcatga cggcgacaga aaacgcggca atgacgcggt
    21001 ctaccgcaag tttcaggcgg ttaaatatca ttgtaagcat cctggaaaat cagggccaga
    21061 gactggcccg gaatattatt gagcagcgtt gtcgaacttc tctaacaaag cagcttgttt
    21121 tggatctttt ttgaagtcgt caaacagagg ctgaactgcg gcacggaatg gtgctttatc
    21181 cacttttaca atttcacccc ccatagcttt cgcctgagca cgtgtgtcag cgtcaatttt
    21241 ttcccacaat ttttgttgat aagcttcgga tttcttcgct gctgtttcga ggatttgttg
    21301 ctgttctggg gttaatttat tccatgtttt ggtggagata accaaaaagt caggaattga
    21361 ggcgtgttca tcttcagaaa aaactttggc aatttcaata tgtcgagtct gcacccagga
    21421 aggcacgtta ttttctgcgc catctactac accttgctgc atcgcagtat aaacttcgcc
    21481 aaatgaaatt ggagtgggtg aaccacccat cagttcaatc attttgatgg tggtcggact
    21541 tggctgaaca cgaattttca gccctttaag atcttctggc ttagtaatcg gttttttcgc
    21601 atagaaactg cgtgtacccg ctacataagc tgataaagca aaaaagcctt tgtcttttgt
    21661 tgagtccata atttctttac caacttcacc aaaaacaact ttgttgaagt gagcctgatc
    21721 tttaaataag aacggcaaat tatagatagc ataaatatta tcaaaagact ccagatcact
    21781 ggcggaacct ttggtcatat ccagagcgcc attttgcaac aactccatag tttctcgcgc
    21841 attacccatc tggctgctgg gataaatacg gatcaccata ttaccttttg acagctgttt
    21901 tacctctttc gccagctctt caaaactctg atgtacaaca tgacttcttt ccaggttgtg
    21961 tgctaacttt aacgtcactt tttctgcggc gtttacgcca gtgctaacca gagccagcaa
    22021 ggatgcgcag actaatgagc gggtaaatga cctcattttc ataaaaaaac tccctgttga
    22081 aggatggaaa ttggttgata tctaattgtg cttatcagtg ctttgtgcga caaattggtt
    22141 aacctgtctg acaacttgtc tatgtttatc acaacttgaa ataaagtcag aaattaaa
//
LOCUS       DAZFRN010000075        21909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFRN010000075 DAZFRN010000000
VERSION     DAZFRN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.075"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(46..2115)
                     /gene="glyS"
                     /locus_tag="ACHKUB_002769"
     CDS             complement(46..2115)
                     /gene="glyS"
                     /locus_tag="ACHKUB_002769"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO6655755.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YAERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2125..3036)
                     /gene="glyQ"
                     /locus_tag="ACHKUB_002770"
     CDS             complement(2125..3036)
                     /gene="glyQ"
                     /locus_tag="ACHKUB_002770"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO6655756.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3131..3430)
                     /locus_tag="ACHKUB_002771"
     CDS             complement(3131..3430)
                     /locus_tag="ACHKUB_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO6655757.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3605..4600
                     /gene="wecH"
                     /locus_tag="ACHKUB_002772"
     CDS             3605..4600
                     /gene="wecH"
                     /locus_tag="ACHKUB_002772"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO6655758.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4642..5079)
                     /gene="yiaA"
                     /locus_tag="ACHKUB_002773"
     CDS             complement(4642..5079)
                     /gene="yiaA"
                     /locus_tag="ACHKUB_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO6655759.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGYYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5125..5466)
                     /gene="yiaB"
                     /locus_tag="ACHKUB_002774"
     CDS             complement(5125..5466)
                     /gene="yiaB"
                     /locus_tag="ACHKUB_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO6655760.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5635..7089)
                     /gene="xylB"
                     /locus_tag="ACHKUB_002775"
     CDS             complement(5635..7089)
                     /gene="xylB"
                     /locus_tag="ACHKUB_002775"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO6655761.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7161..8483)
                     /gene="xylA"
                     /locus_tag="ACHKUB_002776"
     CDS             complement(7161..8483)
                     /gene="xylA"
                     /locus_tag="ACHKUB_002776"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO6655762.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8849..9841
                     /gene="xylF"
                     /locus_tag="ACHKUB_002777"
     CDS             8849..9841
                     /gene="xylF"
                     /locus_tag="ACHKUB_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6655763.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9919..11460
                     /gene="xylG"
                     /locus_tag="ACHKUB_002778"
     CDS             9919..11460
                     /gene="xylG"
                     /locus_tag="ACHKUB_002778"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO6655764.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11438..12619
                     /gene="xylH"
                     /locus_tag="ACHKUB_002779"
     CDS             11438..12619
                     /gene="xylH"
                     /locus_tag="ACHKUB_002779"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO6655765.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12697..13875
                     /gene="xylR"
                     /locus_tag="ACHKUB_002780"
     CDS             12697..13875
                     /gene="xylR"
                     /locus_tag="ACHKUB_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO6655766.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(13983..14807)
                     /locus_tag="ACHKUB_002781"
     CDS             complement(13983..14807)
                     /locus_tag="ACHKUB_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO6655767.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15127..17157
                     /gene="malS"
                     /locus_tag="ACHKUB_002782"
     CDS             15127..17157
                     /gene="malS"
                     /locus_tag="ACHKUB_002782"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6655768.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17335..18588
                     /gene="avtA"
                     /locus_tag="ACHKUB_002783"
     CDS             17335..18588
                     /gene="avtA"
                     /locus_tag="ACHKUB_002783"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO6655769.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEEGVKILAEEIERAWAESH"
     gene            complement(18740..19213)
                     /locus_tag="ACHKUB_002784"
     CDS             complement(18740..19213)
                     /locus_tag="ACHKUB_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6655770.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19315..20163)
                     /gene="yiaJ"
                     /locus_tag="ACHKUB_002785"
     CDS             complement(19315..20163)
                     /gene="yiaJ"
                     /locus_tag="ACHKUB_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO6655771.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20364..21362
                     /gene="yiaK"
                     /locus_tag="ACHKUB_002786"
     CDS             20364..21362
                     /gene="yiaK"
                     /locus_tag="ACHKUB_002786"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO6655772.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21374..21841
                     /locus_tag="ACHKUB_002787"
     CDS             21374..21841
                     /locus_tag="ACHKUB_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO6655773.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKGLDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         5525 a         5569 c         5382 g         5433 t
ORIGIN      
        1 ataaaaaaag cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga
       61 aatatccgca acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag
      121 acgcaattct ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc
      181 acgcagctct gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc
      241 cagcttgtca cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag
      301 ggtagaggca ttcacgcggt cgctcagcac ttcgtcagat ttcgccaaaa tgttggagac
      361 acgcttgttc gccgccgcca gtgcagcagc tgcttcaagg gtacggaagt gcgataccgc
      421 tttcatgcgg gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat
      481 ggtgtcaacg gtgtaacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc
      541 gataacatca tcaaccacgt tggcgttagt cagcttatcg ccatacagac gcactgcttc
      601 ttcggtcagc gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc
      661 aagtgcggca cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct ggccgatacc
      721 gaagataccc gccagagtat ccatcttgtc agcaatcgcc agcgcacagg ctaccgggtt
      781 agacggcagg tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg ccaccgcgac
      841 atcttccgct tcgccatcgt gacgcgcata gtgcatcccc ataacgccct gggtgtcggt
      901 gaactcgaag accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc
      961 gtggttaacg tcagcgccaa tctgttcagc aatccagcca gccagcgcct ggatgcggtc
     1021 agttttgtca cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag
     1081 gttatcttcc agacgttttt tacggtcggt gttgaagaag aactcggcat ccgccagacg
     1141 cggacgaacg actttctcgt taccggagat aatttgctgc ggatctttcg attcgatgtt
     1201 ggcaacgaag atgaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtattt
     1261 ctggtcacct ttcatggtgt aaaccagcgc ttcagccggc accgcgagga atttctcttc
     1321 gaattttgcg gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct
     1381 ttcgcttaag tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat
     1441 cttcgcttta cgttctgcgt aatcggcgat gactttccca cgctcacgca gaatttccgg
     1501 atactgatcg gcgttatcga tggtgaattc cggctcgccc ataaagcggt ggccgcgaat
     1561 cacgcgatcg gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag
     1621 ggtcacggtg tgcaccggac gcacgaagtg cacgtcgctt gcgccccaac gcatcagttt
     1681 cgggatcggc agtttcgcca gagaagtcgc aaccatattc ggcagcagtg cttcggtgct
     1741 ttcgcccttc acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc
     1801 agcctggtca acggtaatac cgcaaccacg cgcccaacct tctgccgctt tgctcggttt
     1861 gccttcagcg tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatccgg
     1921 ttgcgcttcc gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg
     1981 aacggtgccg tgtgcgaggc cagcgttatc cagctccgca gtaaaattcg cagcaaagga
     2041 ctcagccagg ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa
     2101 agttttctca gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc
     2161 ttcacgggaa gcgtagtatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat
     2221 gtagcgctga cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg
     2281 ggcggctttc agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag
     2341 ctgctgcgct tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta
     2401 ttcgaagttg taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt
     2461 tttacccagc gggccgtcgc tccagaccag gtcgtaaacg ctgtctacgc cctgaatgta
     2521 catggccaga cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc
     2581 accaacctgc tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca
     2641 gcccagtccc caagcaccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc
     2701 gtgaatagtc gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt
     2761 gtccggcgat ggcttaatga ccacctgaaa ctgatagtag tgctgtaaac ggttggggtt
     2821 ttcgccgtag cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg
     2881 ttctggcccc agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat
     2941 gtccaatggt tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat
     3001 caagccctgg aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg
     3061 gatttaaagg aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa
     3121 aaagcagggc ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga
     3181 caaactgctc tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga
     3241 aggcggtgac gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc
     3301 ataacgcttc catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca
     3361 cgggtgacgg tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt
     3421 tcatcatcat taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag
     3481 tggctgatat cttacgaatt tccttaagcc tctataagct gtagtgataa aactcagatt
     3541 attccagtgc gccaactgaa tgatttatcg gtcagacttt ttttaaccag atacagagac
     3601 acagatgcag cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt
     3661 ggtgatgatt cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac
     3721 ctgggatatc gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat
     3781 gatatccggc tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat
     3841 cggcttatgc ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc
     3901 catcaatatg gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg
     3961 gtttttcttc gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt
     4021 cggcggaaaa atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac
     4081 agtgccgcag aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga
     4141 tactttttac tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca
     4201 gcataaagca ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc
     4261 tcgcgggaca ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta
     4321 ctgtgggccg atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct
     4381 ggatacgcgt accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg
     4441 attccacgcc ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc
     4501 aatactggat attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat
     4561 gctggtacaa cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg
     4621 gactgaccgg gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt
     4681 tggcgggttt attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct
     4741 taagaagaaa gccagtccat aataaccttt ttcactgagt aataatgtcg cattccacag
     4801 acctaccatc agtaatgcaa cagagataat aaagacagtc aggcaggtca tataataaat
     4861 ggaagtggtc ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga
     4921 aaacagtcct aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc
     4981 attccatagc cctaacagat aggtaacgat accaccaacg agagctatcc atgacacaat
     5041 actaaaggcc ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct
     5101 gaatgtgtaa gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa
     5161 gcagacaaaa aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa
     5221 aacaccgata aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa
     5281 aaaattatcc agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc
     5341 tgtcattaac acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag
     5401 cccaaccaga taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt
     5461 tttcataacg atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa
     5521 ctgaaaaatg ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt
     5581 agtgagatat atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc
     5641 cattaatggc agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc
     5701 ggcataacgc tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc
     5761 aatgagcgat ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg
     5821 ccccacatcc ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg
     5881 acgccagtac tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc
     5941 gcaggcatgc acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc
     6001 tcgcgccagt tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg
     6061 gggattattg tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc
     6121 actttcatca gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa
     6181 tttcgcgacc caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg
     6241 ctgcggtagc gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc
     6301 gctgacagca aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac
     6361 cattcccaca ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt
     6421 cgccataccc cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc
     6481 atataatgcg ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca
     6541 gtcacgcttt gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa
     6601 ctcccccgtc atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg
     6661 gaatatctcc ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat
     6721 catcaggttg ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc
     6781 ttgcgcacag cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc
     6841 cagtaaggtt gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga
     6901 atgctgatcg cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc
     6961 ttgttccgac cagagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac
     7021 cacctcaccc tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc
     7081 gatatacata tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca
     7141 acggactgca cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt
     7201 tgctcctggc gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct
     7261 aaatctgcca gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg
     7321 gaataacgct gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt
     7381 ttcagcgcca gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta
     7441 tcagtacttt gacgacgtac tttggcatcg aagttcaaac caccggtggt gaaaccgcct
     7501 gctttgagaa tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg
     7561 gtgtcccagc ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc
     7621 gcaatggcgg tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct
     7681 tcaatgttca gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc
     7741 gcggcatcgt aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac
     7801 gtgccctgga agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt
     7861 tgttcacgct cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc
     7921 cacaggacat agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc
     7981 gttgccgccc agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccgtagcga
     8041 gggtttgtaa agcagttggc ggttccccac agcagcttca cgccgctctc ttcttgcttg
     8101 cctgccagga catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc
     8161 tcaggggaaa catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac
     8221 tcaaatgcga catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac
     8281 ggacgattaa acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag
     8341 tagcaggcgg caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga
     8401 ttgtagtgac ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg
     8461 agctggtcaa aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg
     8521 atgatgtcgt aatattgggc actccctttc agttgctcaa ttatgttatt tcacactgct
     8581 attgagataa ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaactggg
     8641 taattcctcg aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt
     8701 cataaatcaa gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc
     8761 ccctgtttag ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct
     8821 gtcctctaac tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg
     8881 cacctcactt ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc
     8941 gattgatgat ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc
     9001 agaatctctc ggcgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat
     9061 gtcgcagatt gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa
     9121 cggccaggta ttaagtaacg ttgtaaaaga agccaaacaa gaaggtatta aagtattagc
     9181 ttacgaccgt atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa
     9241 agtcggtgaa ctgcaggcaa aagccctggt cgatattgtt ccgcaaggta attacttcct
     9301 gatgggcggc tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt
     9361 gttaaaacct tacgttgatt ccggaaaaat taaagtcgtt ggtgaccaat gggttgatgg
     9421 ctggttaccg gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa
     9481 aattgatgct gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag
     9541 cgcgcaaggt ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat
     9601 taaacgtatt gctgccggta cgcaaactat gacggtgtat aaacctatta cgttgttggc
     9661 aaatactgcc gcagaaattg ccgttgagtt gggcaatggt caggaaccaa aagcagatac
     9721 cacactgaat aatggcctga aagatgtgcc ctcccgcctc ctgacaccga tcgatgtgaa
     9781 taaaaacaac atcaaagaca cggtagttaa agacggattc cacaaagaga gcgagctgta
     9841 agcgttacgc cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt
     9901 ggcttaggtg gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc
     9961 ggcagtgtga aggcgattga taacgtctgc ttgcggttga atgctggcga aatcgtctca
    10021 ctttgtgggg aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat
    10081 ccccatggct cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac
    10141 atccgcgata ccgaacgcaa aggtatcgcc atcattcatc aggaattggc cctggtgaaa
    10201 gaattgaccg tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg
    10261 gattatgacc tgatgacgct acgctgtcag aagctgctcg cacaggtcag tttatccatt
    10321 tcacctgata cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc
    10381 aaggcactta ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact
    10441 gagcaggaaa cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc
    10501 tgtatttata tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt
    10561 attcgcgacg gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt
    10621 atcaccatga tggtcgggcg agagttaacc gcgctttacc ctaatgaacc acataccacc
    10681 ggagatgaaa tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt
    10741 aaacgagtta atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga
    10801 ctcgttggtg ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa
    10861 tgggaaggaa aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc
    10921 atcgcccagg ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta
    10981 atggcggttg gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc
    11041 cagcttgatg acgcggaaga gcaaaaatgt attctggaat caattcagca actcaaagtt
    11101 aaaacgtcgt cccccgatct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg
    11161 atcctcgctc gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg
    11221 ggtatcgata ttggcgcgaa atacgagatc tacaaattaa ttaaccaact cgtccagcag
    11281 ggtattgccg ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgg
    11341 gtactggtga tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag
    11401 gagcaggtga tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga
    11461 agtgaaattg gccgtaccga catccggtgg cttttccggg ctgaaatcac tgaatttgca
    11521 ggtcttcgtg atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga
    11581 tggtgcctac ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg
    11641 catcctcgcg gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg
    11701 ctcaatgatg gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc
    11761 tttgccactt accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg
    11821 atggtgggtc gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc
    11881 atttcgcggc atactcattg gcatcaccaa cggcacgact gtttccccca ccagcgccgc
    11941 gatgtcacaa attgggcaaa gctatctacc tgccagcact ggcttcatca ttggcgcgct
    12001 tggcttaatg gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg
    12061 tttgcagtct ccggcctcta ccgcagtagt cggtcgccag gctttaaccg ctatcatcgt
    12121 attaggcgca atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct
    12181 gacgttgctg ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat
    12241 ttatgccatc ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac
    12301 caaacttgcc gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag
    12361 ttctcgactt ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc
    12421 agcatgcgtg attggcggca ccagcctggc tggcggagtg ggaagcgttg ccggagcagt
    12481 aatgggggca tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac
    12541 cttctggcag tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc
    12601 aaccaaacgc cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg
    12661 ttttgagttt ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc
    12721 acattactgt tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa
    12781 tatttacagg cgtcacaatc ggaatgggat attttcattg aagaagattt ccgcgcccgt
    12841 attgataaaa tcaaggactg gttaggagat ggcgtcattg ccgacttcga cgacaaacag
    12901 atcgagcaag cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac
    12961 cttgcagaaa gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa
    13021 agcgcatttt tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg
    13081 gaatcaagcg gcaaacgttg ggccactgag cgcgaatatg catttcgtca gcttgtcgct
    13141 gaagaaaagt atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa
    13201 cacgcgcaaa atcggctggc agactggcta caaacgctgc caccgcaaac cgggattatt
    13261 gccgttactg acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc
    13321 gtaccggaaa aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg
    13381 cgtgtcgccc tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa
    13441 ctgttgcatc gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca
    13501 gttcgcgtca ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt
    13561 caggccatgc attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg
    13621 gatgcggtcg ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa
    13681 accatccatg ccatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca
    13741 accaccttgt cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc
    13801 tactctgttt ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc
    13861 gaggtcatgt tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc
    13921 cacctgatgc gagcaaaagc cttatccgac cgacaaatca gataaggctc aaggaaatgc
    13981 aattacatat gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagtta
    14041 ttataactct tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc
    14101 acttcctgat ccgctttacg cagctgcgca cgcgatttac ggaacgaaga gtaagccggg
    14161 tgcgtattca ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag
    14221 taccctttca ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac
    14281 agattgttat tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc
    14341 gtcgccacca tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt
    14401 tttcgcgtat taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca
    14461 cgctcagcag gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca
    14521 gtaatggctg cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc
    14581 cttggtgttc cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg
    14641 cgagaatact cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt
    14701 tgactcgctg ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt
    14761 aacataagaa tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt
    14821 cggatttaat cattccaaca ccttatattt ttcacaaatt tgagagttga gtctcaaatc
    14881 atatcaaaaa tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac
    14941 gtgttaacga ttcagattgg cgctaaatcg cagaaaatgt ggggtttatc gcaaaattca
    15001 gccgtttttt gcgcgagatc gctcaccctt gcttctcatc ctgtggactt accgctcagg
    15061 gatgagtttt gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca
    15121 tccaccatga aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc
    15181 agctggactt ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc
    15241 cacgcgcagt tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg
    15301 cagcctgcag atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg
    15361 ccgcctcaat ggcgattgtt cagagacggc aaatatacgc tgcaaataga cacccgctcc
    15421 ggtacgccaa cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctagtc
    15481 cgtgaatgcc cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg
    15541 gaaggagccg ccgtacggga ttattacagc cagcaaattg cgatagtgaa gaacggtcaa
    15601 ataacgttac aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact
    15661 gacacatccg cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt
    15721 ttcgaaaacg gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg
    15781 gaaattggca cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc
    15841 cagcagttgg gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg
    15901 gtcggcggcg gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag
    15961 gactggacga atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat
    16021 agcgcacatc agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccggctat
    16081 gccacgctgg cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgag
    16141 gtgaaaaaaa cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg
    16201 catagcttta acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga
    16261 aaaaactgga tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact
    16321 atgtcgctgg cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg
    16381 gtgttctata aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc
    16441 gattacttaa cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg
    16501 gtcgataccg ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc
    16561 gccgcgcttc gcgaatggaa aaaagctaac cccgacaaag cattagatga caaacctttc
    16621 tggatgaccg gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc
    16681 ttcgatgcga tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg
    16741 gcgcagatgg atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg
    16801 agctacctct cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag
    16861 ttattactat tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg
    16921 ttcggtccta caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat
    16981 gttagcggta aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc
    17041 cgccatcccg caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc
    17101 tttgttcgtg agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt
    17161 ttccggcttc ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc
    17221 taatcagcgt tgcaggataa agcaccgctc actcttcaac agaccgattt gcaccccagc
    17281 aaatgtagcg ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca
    17341 ttctcccttt ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat
    17401 ctgaacgacg gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag
    17461 atcccggaaa tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa
    17521 gcgactgatg cactgtgcaa ttacgacggt ccacagggga aaacggagct actcacactg
    17581 cttgccggaa tgctgcgcga gaagttgggt tgggatatcg aagcacagaa tattgcacta
    17641 acaaacggca gccagagcgc gtttttctac ttatttaatc tgtttgccgg acgccgtgcc
    17701 gatggtcggg tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac
    17761 gccggactgg aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa
    17821 ggccagttta aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg
    17881 atttgcgtct cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg
    17941 aagcttgacg cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc
    18001 gtcccgttcc cgggtatcat cttcagcgaa gcgcgcccgc tgtggaatcc gaatatcgta
    18061 ctgtgcatga gcctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc
    18121 aatgaaaaaa tcatcaccgc catcaccaat atgaacggca ttatcagcct ggcacctggc
    18181 ggtattggtc cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct
    18241 gaaacagtca tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc
    18301 cgctatttac cggaagatcg ctgcctaatt cataaaccag aaggagccat tttcctctgg
    18361 ctttggttta aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc
    18421 ggcgtgctga tggtgccggg gcacaacttc ttcccagggc tggacaaacc gtggccgcat
    18481 acgcatcaat gtatgcgcat gaactacgta ccagagccgg agaaaattga ggagggggtg
    18541 aagattctgg cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat
    18601 gcgctacgct tattaggcct acatgatttc tgcaatatat tgaatttgca agaatttgtg
    18661 ggccggataa ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga
    18721 gaaacgccgg aaaaacgttt caaacaggct gctgccgtag ccgatgccgc tgcactttct
    18781 cgacatccat gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc ggtcccgtct
    18841 cccgatgcca gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct
    18901 ccatcgcacc aaacgggcaa gccagcatac agcttttaca gccaatgcaa cgtgtttgtt
    18961 caacgaaaat atgcccatgt tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg
    19021 gtgcatcttc acactgatga caggctactg ccgtggtcca gctgtggtct ttaatgacac
    19081 gaatacggga aataaactcg tctggtgaca acgcagcgca atcctgattc tcctgatgcg
    19141 acactgcgca agccacttca caggtacggc aaccgataca tttcgtcgca tccgcaataa
    19201 taaaccggtt catgatcact cctttgcact aaaacagcag agtgccagag agcgcgtccc
    19261 tactgctttg atccaacgaa ggaaatgtta aagttttggc acttgtccaa agcgttatgt
    19321 gatcgcgccc aggtcatccc tgacagtaaa tcccagttca ttagaaatag cctgcgcggt
    19381 ttcacgcagt ggtttcagga gatttttctc tcccacctgt ttcagacgtg atgtcgaaag
    19441 cgaaatcgac acggcgtacg gcacccgccc atgaatatca aacaccggaa cagcaataca
    19501 ggagacgccg agttcgtttt cttctctgtc catcgccgct ccgctttcac gaatgtgcgc
    19561 cagttcgtcg aacatcgcgg gcagctcggt aatggtattg cgggttaacg gctggatctc
    19621 atgctgatgg ttttcccagt atgacttcac gtagtccggg tgaccaaacg ccatgtagat
    19681 cttgcccatt gcggaacagt agagcggcat atgctggcca atataggcac gggttcgcag
    19741 catcccggtt gtgggttcca gcttataaat caaaatagcg tgatcgtctt cgcggctgga
    19801 gaagttaatg gtttcaccag tggcgatgtt cagtgcctca agatgcggag cggcgatatg
    19861 aatgatattc agcgaagaca gcgccttctg cccgacggca ataaatttgg tagtcaggcg
    19921 gtaactccct gcggcgggcg cggtggtcac atagccgcag gactgtaatc cctgcaataa
    19981 gcgatggacg gtactcttat ttaaaccagc cagctccgaa agatgcgcca acggacaacc
    20041 gtttggatag ttgctcaaaa tctcaatcag catcaacccg cgaaacagac tctggcttcc
    20101 ggctggacgc tctttttcct gcgccatctc gttctctttt tttcccatca cttctttccc
    20161 cattttgtcg cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata
    20221 atttataacc acttgatttt tatgtctttt gaaattcatc aatcagattg ctttgttaaa
    20281 aagtgatcga tatatttgaa atcaagtttc gcatattgaa attttaagcc aaaaaagcga
    20341 tcaaaaaaac aaggaagcct gggatgaaag tgacatttga gcagttaaaa gcagccttta
    20401 atcgggtctt aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt gcagagatgt
    20461 tcgcccgcac caccgaatcc ggcgtttatt ctcacggcgt taatcgtttc cctcgtttca
    20521 ttcaacaact ggaaaacggc gatatcattc ctgatgccca acccaaacgt ataaccagcc
    20581 tcggcgcaat tgaacagtgg gatgcccagc gttcgatcgg caacctgacg gcgaaaaaga
    20641 tgatggatcg cgccattgaa ctggctgccg atcacggtat tggtctggtg gcacttcgta
    20701 atgccaacca ctggatgcgc ggcggcagct acggctggca ggcggcggaa aaaggctata
    20761 ttggcatttg ctggaccaac tccatcgccg taatgccgcc gtggggcgca aaagagtgtc
    20821 gcattggcac taacccgctg atcgtcgcca ttccttccac gccgatcacc atggtcgata
    20881 tgtcgatgtc gatgttctct tacggcatgt tagaagttaa ccgcctggcg ggccgtcagc
    20941 tcccggtcga tggtggcttt gatgatgagg gcaatttgac caaagaacct ggcgttatcg
    21001 agaagaatcg ccgcattttg ccgatgggct actggaaagg ttctggcatg tcgattgtgc
    21061 tggatatgat cgctactctc ctttccgacg gcgcatcggt tgccgaagtc acccaggaca
    21121 acagcgacga atacggcatt tcacaaattt ttattgccat tgaagtggac aagcttatcg
    21181 acggtcccac ccgcgatgcc aagctgcaac gtatcatgga ttacgttact accgctgaac
    21241 gcgctgacga aaaccaggcc atccgcttac ccggccacga atttactacc ctgctggccg
    21301 aaaaccgccg taacggtatt accgttgatg acagcgtgtg ggccaaaatc caggcgttat
    21361 aaggagataa gtcatgattt ttggacatat cgcgcagccc aatccgtgcc gtttgcccgc
    21421 cgccattgaa aaggggctcg attttctgcg cgctaccgat ttcaacgccc tggagccggg
    21481 cgttgtcgaa atcgacggca aaaatattta tgcgcaaatt atcgatttaa ccacacgcga
    21541 agcggtggaa aaccgtccgg aagtgcatcg tcggtatatc gatattcagt ttctggcgtg
    21601 gggtgaagag aaaatcggca ttgctattga tacgggaaat aataaagtca gcgaatcact
    21661 attagagcag cgcgatatta ttttttatca tgacagtgaa catgaatcat ttatagaaat
    21721 gataccgggc agctacgcaa ttttctttcc gcaggatgtt caccgaccag gttgtatttt
    21781 gcaaacagcc tctgagatac gaaaaattgt ggttaaagtc gcattaacgg cgctgaatta
    21841 atcgaataat cgtctacgcc agaacgactg ggtaatgtta ttgctctaac tatattaata
    21901 atttaggtt
//
LOCUS       DAZFRN010000076        21798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFRN010000076 DAZFRN010000000
VERSION     DAZFRN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.076"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..319
                     /locus_tag="ACHKUB_002788"
     CDS             <1..319
                     /locus_tag="ACHKUB_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6655774.1"
                     /translation="AMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRRED
                     YGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLE
                     SLR"
     gene            409..894
                     /locus_tag="ACHKUB_002789"
     CDS             409..894
                     /locus_tag="ACHKUB_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6655775.1"
                     /translation="MTTVSHNFTTSSVSVTTASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCENYCDGIWNLYTLNNGGAFMAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            910..1386
                     /locus_tag="ACHKUB_002790"
     CDS             910..1386
                     /locus_tag="ACHKUB_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6655776.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1449..1670
                     /locus_tag="ACHKUB_002791"
     CDS             1449..1670
                     /locus_tag="ACHKUB_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6655777.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWQGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1833..2207
                     /locus_tag="ACHKUB_002792"
     CDS             1833..2207
                     /locus_tag="ACHKUB_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6655778.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            2254..2631
                     /locus_tag="ACHKUB_002793"
     CDS             2254..2631
                     /locus_tag="ACHKUB_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6655779.1"
                     /translation="MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            2628..3116
                     /locus_tag="ACHKUB_002794"
     CDS             2628..3116
                     /locus_tag="ACHKUB_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6655780.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LHVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3128..3325
                     /locus_tag="ACHKUB_002795"
     CDS             3128..3325
                     /locus_tag="ACHKUB_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6655781.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            3410..4276
                     /locus_tag="ACHKUB_002796"
     CDS             3410..4276
                     /locus_tag="ACHKUB_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6655782.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLIDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            4348..4611
                     /locus_tag="ACHKUB_002797"
     CDS             4348..4611
                     /locus_tag="ACHKUB_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO6655783.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            4608..4934
                     /locus_tag="ACHKUB_002798"
     CDS             4608..4934
                     /locus_tag="ACHKUB_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6655784.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(5348..5884)
                     /gene="yghD"
                     /locus_tag="ACHKUB_002799"
     CDS             complement(5348..5884)
                     /gene="yghD"
                     /locus_tag="ACHKUB_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO6655785.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETILQQLVAMNTRLKSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(5886..7064)
                     /gene="gspL"
                     /locus_tag="ACHKUB_002800"
     CDS             complement(5886..7064)
                     /gene="gspL"
                     /locus_tag="ACHKUB_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO6655786.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(7061..8039)
                     /gene="gspK"
                     /locus_tag="ACHKUB_002801"
                     /pseudo
     CDS             complement(7061..8039)
                     /gene="gspK"
                     /locus_tag="ACHKUB_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
     gene            complement(8042..8641)
                     /gene="gspJ"
                     /locus_tag="ACHKUB_002802"
     CDS             complement(8042..8641)
                     /gene="gspJ"
                     /locus_tag="ACHKUB_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO6655787.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(8638..9009)
                     /gene="gspI"
                     /locus_tag="ACHKUB_002803"
     CDS             complement(8638..9009)
                     /gene="gspI"
                     /locus_tag="ACHKUB_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO6655788.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(9006..9569)
                     /gene="gspH"
                     /locus_tag="ACHKUB_002804"
     CDS             complement(9006..9569)
                     /gene="gspH"
                     /locus_tag="ACHKUB_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO6655789.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASSGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(9573..10028)
                     /gene="gspG"
                     /locus_tag="ACHKUB_002805"
     CDS             complement(9573..10028)
                     /gene="gspG"
                     /locus_tag="ACHKUB_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO6655790.1"
                     /translation="MYSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(10045..11268)
                     /gene="gspF"
                     /locus_tag="ACHKUB_002806"
     CDS             complement(10045..11268)
                     /gene="gspF"
                     /locus_tag="ACHKUB_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO6655791.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(11268..12761)
                     /gene="gspE"
                     /locus_tag="ACHKUB_002807"
     CDS             complement(11268..12761)
                     /gene="gspE"
                     /locus_tag="ACHKUB_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO6655792.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(12761..14821)
                     /gene="gspD"
                     /locus_tag="ACHKUB_002808"
     CDS             complement(12761..14821)
                     /gene="gspD"
                     /locus_tag="ACHKUB_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO6655793.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGQGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(14851..15810)
                     /gene="gspC"
                     /locus_tag="ACHKUB_002809"
     CDS             complement(14851..15810)
                     /gene="gspC"
                     /locus_tag="ACHKUB_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO6655794.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(15828..16238)
                     /gene="gspS2"
                     /locus_tag="ACHKUB_002810"
     CDS             complement(15828..16238)
                     /gene="gspS2"
                     /locus_tag="ACHKUB_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO6655795.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(16304..17113)
                     /gene="pppA"
                     /locus_tag="ACHKUB_002811"
     CDS             complement(16304..17113)
                     /gene="pppA"
                     /locus_tag="ACHKUB_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO6655796.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            complement(17261..>21798)
                     /gene="sslE"
                     /locus_tag="ACHKUB_002812"
     CDS             complement(17261..>21798)
                     /gene="sslE"
                     /locus_tag="ACHKUB_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO6655797.1"
                     /translation="SLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPT
                     PNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDE
                     SSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNK
                     KSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVEN
                     NAATDKAPSTHTSPVVPATTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGRLVDSQ
                     GDGVVGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGD
                     EVRGANIDQLIHRYSKAGQNHTRVVPDEVRKVFAEYPNVINEIINLSLSNGATLGEGE
                     QVVNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLW
                     GVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGE
                     KRAWDKNELAYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVIGNPHYNSIL
                     RCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKSSMTVGTNLD
                     TVYFKKHGQVLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNGFEYVTQWGS
                     DPYSIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASGFVRLLDA
                     AGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDTTKEVIWK
                     YQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKQRILDAFPGL
                     EVCKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLDLGADTAKAMLQAADLGTNIQ
                     RLYQHELYFRTNGLQGERLNSVDLERLYQNMSVWLWNETKYRYEEGKEDELGFKTFTE
                     FLNCYTNNAYVGTQCSAELKKSLIDNKMIYGEESSKAGMMNPSYPLNYMEKPLTRLML
                     GRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEV
                     TIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLI
                     YIKGDSKDNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTAPKKNLN
                     ASNYTGGLKQFANDLDTFASSMNDFYGRNEEDGKHRMFTYKNLTGHKHRFANDVQISI
                     GDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLA
                     LYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDI
                     KKWYPEGELPKFFSDREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCAESNGNAADT
                     LMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNTLASLKLPKPE
                     QGPETINKVTEHKMSVE"
BASE COUNT         5011 a         6135 c         5465 g         5187 t
ORIGIN      
        1 tgccatgcag tcgctgattc tgccgccacc ggcacgccag gcgctggcac aggcggcact
       61 gacttaccgt tatggtgacg aacatcagcc cgtcaccacc gccgacattc tgacgccacg
      121 acgccgggag gattacggta aggacctgtg gagtgcttat cagaccattc aggagaatat
      181 gctgaaaggc ggaatttcag gtcgcagcgc taaaggaaaa cgtatccata cccgtgccat
      241 tcacagcatt gacactgaca ttaagctcaa ccgcgcattg tgggtgatgg cggaaacgct
      301 gctggagagc ctgcgctgat gccttttccc tgaaagcgca gttctgactc atgtccgtcc
      361 cggcccgcga cttcttccat tcatttactt tttataagga ataatctcat gacaaccgtc
      421 tcgcataatt tcaccacatc ttctgtttcc gtaaccactg catcagggaa taaccagtct
      481 cagttggttg ccaccatagt ccctgatgaa cagcgcatca gcttctggcc gcagcacttt
      541 ggcctcattc cacagtgggt caccctggag ccccgtatct tcggctggat ggaccgtctt
      601 tgcgaaaact actgcgatgg tatctggaat ctgtacaccc tgaacaacgg cggggcattt
      661 atggcccccg aaccggatga tgatgatgga gaaacatggg tactgttcaa tgccatgaac
      721 ggtaaccgcg ctgaaatgag cccggaagct gccggtattg ccgcctgtct gatggcgtac
      781 agccatcacg cctgtcgtac ggagtgttat gccatgacgg tccattatta ccggctgcgg
      841 gattacgccc tgcagcatcc ggaatgcagc gccattatgc gcatcatcga ctgaatggag
      901 gcagggacaa tgcagcagct ttcctttctg cccggagaga tgacgcccgg cgagcgcagt
      961 ctcattctgc gggccctgaa aaccctggac cgccatcttc atgaacccgg cgtggccttc
     1021 acctccaccc gagcggcacg ggaatggctg attctgaaca tggcgggact ggagcgtgaa
     1081 gaattccggg tgctgtatct gaacaaccag aatcagctga ttgccggtga aaccctcttt
     1141 accggcacca tcaaccgcac ggaagtccat ccccgggaag tgattaaacg cgccctgtac
     1201 cacaatgccg cagccgtggt gctggcacac aatcacccgt ccggtgaagt cacccccagt
     1261 aaggcagacc gccttatcac ggaacgtctg gtacaggcac tgggcctggt ggatatccgg
     1321 gtgccggacc atctgatagt cggtggcagc caggttttct cctttgccga acatggtctg
     1381 ctttaacccg tcacaaccac atcacacctg ttttcacttt tatcttctgt cttcagaggt
     1441 atcccattat gaaaattatc acccgtggtg aagccatgcg cattcaccgg cagcatcctg
     1501 catcccgtct ttttccgttc tgcaccggca aataccgctg gcaaggcagt actgaagcgt
     1561 ataccggtcg tgaagtacag gatattcccg gtgtgctggc cgtgtttgcc gaacgccgta
     1621 aggacagttt tggcccgtat gtccggctga tgagcgtcac cctgaactga tgttccgtca
     1681 cccggaaaac gagtacgacc agatggactc agacgccgct gtggatgagg ccattctcgt
     1741 caatgcatac atcttcaccg aaaccggtca gcgcgccggc tgacctgtca tcgttaaacc
     1801 attcttaacc acttttcaga gaggatttta tcgtgtcaga caaactctcc gggataactc
     1861 atcccgacga caatcacgac cgcccctggt gggggcttcc ctgcacagtg aggccctgtt
     1921 ttggtgcccg tctggtgcag gagggtaacc gcctgcatta ccttgccgac cgcgccggta
     1981 tcagaggccg cttcagcgac gcagatgcat accatctgga ccaggccttt ccgctgctga
     2041 tgaaacaact ggagctcatg ctcaccagcg gtgaactgaa tccccgaaat cagcataccg
     2101 tcacgctgta tgcaaaaggg ctgacctgcg aagccgacac cctcggctcc tgtggttacg
     2161 tttatctggc tgtttatccg gcacaggcac ccgcaaccac ctcataaccc cgaagctctc
     2221 acctttagca ctaccagtta cagagaactt aagatgaaaa cattatccga cacacatgta
     2281 cgggaggcat ctcgctgccc gtctcccgtc accatctggc agacactgct catccgactg
     2341 ctggaccagc attatggcct cacactgaat gacacaccgt ttgccgatga acgtgtgatt
     2401 gagcagcata ttgaggcagg catttcactg tgtgatgcgg tgaactttct cgtggaaaaa
     2461 tacgcgctgg tacgaactga ccagccagga ttcagcgcct gtacccgctc tcagttaata
     2521 aacagcatcg atatcctccg ggctcgccgg gcaacaggcc tgatgacccg cgacaattac
     2581 agaatggtaa ataacattac ccagggtaag catccggagg cgcaacaatg aaactggccc
     2641 tgacactgga agccgacagc attaacgtgc aggcactgaa catggggcgc attgtcgttg
     2701 acgtcgatgg cattgagctc gctgaactga ttaacaaagt cgctgaaaac ggttattcac
     2761 tccatgtggt ggacgactcc gaccaacagt caacctgcac accaccaccg ttaacaaccc
     2821 ttaccggcat acgctgcagc accgcacata tcacggaaac agacaacgcc tggttgtact
     2881 cgctgtcaca ccagaccagt gacttcggcg aatcagaatg gattcatttt acaggtagcg
     2941 gatatctgtt acgtaccgat gcgtggtcat acccggttct gcggcttaaa cgcctggggc
     3001 tgtcaaaaat gttccgccgt ctggttgtca cacttatccg gcgttatggc gtcagtctca
     3061 ttcatctgga tgccagcgct gaatgcctgc cgggtttacc cactttcaac tggtaaccag
     3121 aaacaacatg aaatcattaa ccactgaaac agcgctggat atcctcattg cgtggctgca
     3181 ggacaatatc gactgcgaat cgggaattat ctttgacaac gatgaggata aaacggattc
     3241 ggcagcactg ttgccctgta tcgaacaggc ccgggaggat gtccgtactc ttcgtcatct
     3301 gcagcttctg caccagaacc ggtaaatatc actcaccata cttcattcca ctgacgccag
     3361 cctgaacagg gctggcgttt tcatttatct gcaaaaagga aaaacgatta tgtcaggtat
     3421 cacaatctcc cgtcctgaag tggtcaacgg gcatacggac gttatctgtt ccacctcaat
     3481 cggacacatt ctggctgtac gaaagaccac actgatgcaa atcgacacac ttatccggca
     3541 actgacagaa atctcagcaa tgacagaaag tattggaggt aaaaccgccc cggactgggc
     3601 catgaaacag aattttcgct gcggttgctg gctgatggag aaacccgaaa cagcaatgaa
     3661 agccattacc cggaatcttg accgcgaaat ctggcgtgac ctgatgcaac gttccgggat
     3721 gctgtcatta atagatgcac aggcacgtga tacatggtac cggtcactgg aatacgataa
     3781 ttttccggaa atcagtgcgg caaacattct gagcacattt gaacaactgc accagaataa
     3841 ggatgaggtg tttgagcgag gggtgatcaa cgtcttcagg ggactgagct ggaattacaa
     3901 aaccaactca ccctgtaaat ttggcagtaa aattatcgtc aacaacctgg tgaggtggga
     3961 ccggtggggg tttcatttta tcaccgggca acaggcagat cgacttgccg atctggaaag
     4021 aatgctacac ctgttcagcg gcaaaccgat ccccgacaac cgggaaaaca tcacccttcg
     4081 tctggatgat catatccagt ctgttcaggg taaagaggac tatgaagacg agatgttcag
     4141 tattaaatac tttaagaaag gctcagtaca catcacgttc aggaaaccag aactggttga
     4201 caggcttaat gatattattg ccagacacta cccggaagcc ctggcaagca cagtgaacaa
     4261 aagccgcaag gcataaaaga ggtattttac aattcgcgaa tattaactat tattaattga
     4321 tagcaatcaa tcggcgagaa aatcaccatg cctaccagtg tatccctcag tccttacttt
     4381 gaaacgttta tccgcgaaca aatagaaagt ggtcggtata acaatacaag tgaagtcatt
     4441 cgtgccggac ttcgtgctct ggaagaacgg gaacagcaga taaagcttga atcactccag
     4501 agcgccgtta ccgcaggcat caacagcggt gaaagtaaaa gtgctgaaga ggtattcggg
     4561 cgactgacgc acaaatacaa aaagatggca gaaggtgaac agccaatatg aaactgactg
     4621 tttcaccatt agcagaacac gatcttgaag ctataggtga ctggatcgct caggataatc
     4681 ctgtccgtgc aatcagtttt accgaagagc tttaccaaca atgtctgttg atagccgaat
     4741 cgccagtact ctacagagaa agacccgaat taggggctgg gatcagaggt tgttcgtatg
     4801 gtcgttacct tctgctgttc agggtactgg atactgaagt tcgtatcgaa cgtatcgttc
     4861 atggttcccg ggatatcaac agggtacttg cagagccaga cgacaaacca tcagatcatt
     4921 tctggcagcc ataaacaaaa tctgtgcgtt aacgctccct aacacaggta gccaggtatt
     4981 ttataaatct ctgtattttg ttgttacttc gattccgcaa ttcagcatat cgaaccagac
     5041 aaaatgatac agcaaagcac aaaaaagagg gattatagcg tcaaaagaat tcgaattgca
     5101 catgggggcc tcattggtgg cctcgaaaac aacaaagaca aaacaatcga tacaaaacaa
     5161 ttggttatat aaccagtttc attccgagtc cgggcaccac taattcttaa gaacccgccc
     5221 acaaggcggg tttttgcttt tggatctgac aataaccttc acgaaaaaaa ttagcttata
     5281 aagtctgggg gaattactct cgccacgtta acgagagtaa ttttattgat attaatctcc
     5341 tgatacttta cccccgtcca aactccagcc gctgcacatt caccatccca ggcttctcag
     5401 cagcactgac atcaatttgt gtcacccgca gcgcatattt ttcatccagt gcgtttaacc
     5461 atttcagcag gtcattaaac accacaggtt ctatccagac ctgaatattc tccccacgct
     5521 cggctatccg cctgatgact accgagtgcg cggaagcact gtcactgata acccgcgata
     5581 cctgcgcagg cgttgttgtg gcggattttc gcgccgcaat aatatccggc gcggcgctct
     5641 tcagtcgggt gttcatcgcc accagctgct gcaatattgt ctcctgttgc tcaatccgtt
     5701 cgctcaaagg ctgccagatg aggacgtaat atccgacgct aaacaggaac accaccgctg
     5761 ccagtaacat gcccttttca cgcggagaac gccccgccag gtgttgtgcc agccagtgtt
     5821 cgccacggct taactggcgt tcacgccatt gctgaaaata gtgaataaat ttatcgcgta
     5881 acatgttatt tcctccgcaa cgttacgccg ccggaaaccg catcaccctc tttctgtaac
     5941 gcgtcctgct gcacaacata atcggttgcc agtgcgctac gcagtttgtc gaagctggca
     6001 aagttcgcgg cccgcaactg gaggtgaagc gtctggcgtt tttgatcaaa ggtgaaacca
     6061 cgcatttcga tgtcggaaag tgacgctgat ttcagggtgc tggctatcgc tgacaactcg
     6121 gcgagcagcc gggtatcgtc ggcctgtggg cgatattttt tcagcgccat cgtcacctga
     6181 gagcgtaaat tcacaatccg cttctgctcc gggaacaacg ttaagaactg ttcctccgcc
     6241 tgggtgcggc tttgcgccac ctgttcgctg acgctccata acgtcacgcc ccgctccact
     6301 gccagcgcaa ccagaatcag caatatcggc agaatcatca cccgccagcg cgcccactgt
     6361 tttcggtagc tgacgcgagg ctgccacggc cctgtcagca ggttcccttc cggttcgcca
     6421 taagtggtaa cggcgggcag agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat
     6481 gctgatagct tttccggtgc aatgccgact acggttagcg aaagcggtaa atcctgctca
     6541 ttgagctggg cgcggaacat gaccggagcc agcgccagcc cggcgctcca tccccggcat
     6601 tcatcgatgc ggcagataac ccgttgcgca tcgccagcca taaacccaca aggaatggac
     6661 atccagtccg gcgcgacggt agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg
     6721 ttgcgcatat gctgttggtg aatcactgct acggttgcca gttgctggtc gattttcaac
     6781 ggggcgaaat gcagttcatc gatatcctgg ttcagctctt cttccagtaa ggcgggcaga
     6841 atagtcggta tctgcttgcg ggcgacaaca ggcagttcaa cctgccagac gctgatccat
     6901 tcgccgggaa tgtagagtcg aatcgcatca gtttgcagcc attgctggag ctgttcgtta
     6961 gcaacgtcag accagatacc gtgctccaca tcgggtgtac ggcgctgcca atggatggga
     7021 tcagcggcgc aaagcgggaa aaaaatctca aggatggaac tcactcactt tctcctgtct
     7081 gatgccagag aaccgaaaag tgttgtgggc ccatgcggac aattaacgag ttcatcgtca
     7141 gttcagtctc attcacggtg atatcagaac gcagccagaa gtaattgctg tccacgctca
     7201 ggacggtttt tagctgtttt ttagtacgct catcgacgtc agcgagtagc ggctgtgcaa
     7261 gaaactgatc gacatcttcc cagcccttcg ccggacgttg ttgtaataac gcccgcgcct
     7321 gaacagggct taaccacggg tcaaacagcg cctcaagaat cacactttgc gtgacgtcta
     7381 aagtattgat gttgatttgc tggcgggtca tcggcagcgc acagaccagc ggtttcagtt
     7441 tttgataaag cccggcgtcc attccctgca ccacgcgcat ctcgctgata tcggccagcg
     7501 gttgattggc ggcgtagaaa ggcaccgagc gggcgagata ttcgctgtct tcacggccca
     7561 gacgcgtctg cacgctgcgg tcttcatcaa taacctccca caggctttcg gctatcagtt
     7621 cggcccgata agcaggcaca tccaggcgcg tgatcagggc aatcagttgt tgtaccgcga
     7681 gcggacgcga cgccgttgtg ggctgagcga gggcattcag gttaaagcag gcctgtgcgt
     7741 cacgcagagt gacggcgatt ttgcccctgc ggcagtggga aaaaacgagg gccggaagcc
     7801 cacggttgtg ccagatgcac gcgcttttca tttttcaggc tcagactgag cgcgctcaac
     7861 gccaggcttt ctgcactggc gctgtaccac agcgcctgct ggtactcctg ctggctgcgc
     7921 gttcttccca gttgctgctg catccgcccg gaaagcgtga tggttaccag catcataact
     7981 gccagcaata ccagcaccac aaccagtgcc attccgcgtt ttggtggtga ggtgatcatg
     8041 attattgcgg cccgcgtaac aaccagatgc gttcaatctc gccccattgc ggcgaatgca
     8101 gggtcatgcg taccgccaca gggatcgcct gtactgacga ccagctctcc tgccagcgcg
     8161 tgccgtcgta gaactgcaaa cggagcgaat cggccggaat caacttttgc attgtcggct
     8221 tcacgctgcc tgcggcatcg gtcagcggcc aggctaaccg ttcgagataa ccgtcatgaa
     8281 tgcggtaacc gacggtgagc agattactgc gcggcagacg catcaacggg ttaaccacac
     8341 cgccgcgcac aaaacgcatt ccttcactct cagacgccag cacgccagcg ccagccagta
     8401 acgctggttc acgctggccc tgatcgcctc ttaccggacg cggcatcatt tgggtcagat
     8461 cgtgggtcag aaaactcatc gtttgctgca tgaggtttag tttttgatcg tgtccggcga
     8521 cggcgctatt cacgcgcgtg acgccgttag tcacctgctg cgccatcagt gccagcgagg
     8581 caaaaatggc aattgccacc aacatttcca gtaacgtgaa accagcgcga gtccttctca
     8641 ctgttggcct cccacggcgc taaaccaggc gcgtcgtgac tgaatcaccg acgaaaagtc
     8701 ttcgtgaagg ctgacttcaa tatccaccgc ctggagcagc gcgttgcctg tattcaatgg
     8761 gattgttcgc cagtaccagc ggcttcctgc catatcgctc tcgccctgat gcctgaccgc
     8821 ccgctgctca cgggtcatca ggtagagcag cgcagtctgg ttttcagcta cccagcccgc
     8881 taccgttttt tcctcaagaa tttgctgatt actcagcgca ccgctggcaa tctgtaacac
     8941 cgccgtggca gccagcgcaa aaatcgccag cgcgagcatc acttccagca aggtaaatcc
     9001 gcgcttcatg gcatcctctc atcacattga ttgagggata acgcgccatc gtgtgccagc
     9061 tttaccgccc aacatgcgtt ttgcgccgcc gagtagaagc gcagcgtaaa cggcgtggcg
     9121 ggttcaaaag gcgaaaaacg gatctgcggc gtcttctttt tcgcctcttt ttgcagctcc
     9181 agttcaatat cgtgcagcgt caggcgacga cgttgcagct ccagcgcata ctccttctgc
     9241 cagatatcac ttccgggttg taacagcatc tgcacctgtt ttggcaccgt aacctgtgcc
     9301 gataaccggg tcgcggaaac gggcaaccac tgcccctgac gacgctgcat aaactgatag
     9361 ccaggcgcgt cgattagcac accgagtgtc tgcccttcaa tcactgccct gtccttaaac
     9421 tgcgcaaagc gagtcagaaa atcctgtgcc gcttttttcg cgggcggttc agaatcggtc
     9481 gcaaacgtct gtaccacgcc cgaactggca aggccgataa ggaaaatcac caacatgatt
     9541 tccagaagtg tgaatccgcg ttcaggcact gcttactgaa actcctgcaa attccagtta
     9601 ccgatatctg cgccagcgcc ttccccgttt tcctgaccat ccgcccctag ggtataaaca
     9661 tcaaacagtc ctttttcacc cgggctgaga tactgataat cattgcccca cggatccttt
     9721 ggcagtcgtt tgatgtatcc accagtacgg tagttgcggg agtccgccat attggctggc
     9781 tgctggatca gcgcctcaag cccctgttct gtggtcggat aacgcccgtt atccagtcgg
     9841 tacatatcca gcgcattctc cagcgccacg atatcgctga tggctttttg ccgatccgct
     9901 ttctctttat tgcccaacag gttaggcacc accagacttg ccaggacgcc aagaataaca
     9961 atcaccacca tcacttccag cagggtaaaa cctgcccgtg gtttttgtgt gcgggataac
    10021 gaatacatgt gataactccg taaattacat tccaaccata ttgttcagtt gcagcatcgg
    10081 ctcaaggatg gcgatgacga taaacagcac cacgcccgcc atcatcacca ccagcgccgg
    10141 ttcaaacagc cctaacgcca gccccacctg ggtatcaaat tcccgttcct ggttgactgc
    10201 ggcctgctca agcatggttt ccagttcgcc gctctgttcg ccggaggcga tcatgtacag
    10261 catcatcggc gggaacaggc gcaactccgc cagcgcggcg cgcagactgc ttccttcgcg
    10321 gacgcgatct gccgccagca gtagttgttg ttcgacatag cgatttgccg acacagcggc
    10381 agcggtctga ataccttcca gtagcggaac actgctggcg gtgaggatgc ttaaggtgcg
    10441 ggaaaaacgc gccgtattca gcccgcgcgc aacacgccct gtcacgggca gacgcagcac
    10501 ggttttatcc cagcgcaggc gcatagtagg atttttaagt agccgttgcc ccagcaccag
    10561 aagcgccagc aatcccgcca gccagtaaac gccgctggcc tgtaacgcgt cgctcatggc
    10621 aataagcgtg cgggtggagg cgggcagcgc gtgtccgaga tgatcaaact gttcgatgat
    10681 tttcggcacc actgccgtca gcaaaatagt gactacgccc gttgccacca ccagcagaac
    10741 cagcggatag agcatggcct gcaatagacg tgatttcagg cgctgtcgct gttcggtgta
    10801 atctgccagg cgattaagca ccacgtcgag atgcccggat ttttctccgg cagcaaccat
    10861 cgaacaaaac agagaatcaa agacgcgggg atgttcgcgc aggctgtccg acagggtgta
    10921 accttcctga atccggctgc gcagcgccat tccgaggctt tttacatgca gtttttcact
    10981 ttgctcactg accgcctgta agcaggtttc cagcggcatt gctgcctgta ccagcgttgc
    11041 cagttggcgc gtgaacagcg caagatctgc cgccgccacg cgacgatgtg cgtgccgccg
    11101 acgctgcaac atcccccctg acgaagtatt catccgggct tcaatatgca cggggataag
    11161 ctctttaccg cgcaacaact ggcgggcatg acgcgcggaa tccgcctcaa tcataccttt
    11221 ggttttgcga ccattacgct ccagcgcctg atagtaaaac agtgccatta cgcctccatg
    11281 gttacccgca gaacttcatc gagagaggtt tctccggcga gcactttctc aatgccgttg
    11341 ctgcggatac ccgcagagtg ttgtcggaca taacgttcca gctccagctc cccggcctga
    11401 cggtggatca aatcacgcaa tgtggcatcc accacgatca gctcatggat ggcagtccgt
    11461 ccgcgaaaac ctttgtgatt acaggcggga cagccctgtg gatggtacag agtgacggta
    11521 cgggcgtcgg taattcccag caggcgtttt tcttcgtcag tggctggtgc gggctggcgg
    11581 cagtcgggac acagcgtgcg aaccagtcgc tgcgccatca cgcccgtcag actggaagag
    11641 agcaggaaag gctccacgcc catatcctgc aaacgtgtga tcgcccccac cgctgtgttg
    11701 gtatgcagcg tggaaaggac caggtgtccg gtaagtgaag cctggacggc gatttctgcg
    11761 gtttcggtat cgcgaatttc accgaccatc accacatccg ggtcctgacg caaaatcgcg
    11821 cgcagcccac gggcaaaggt catgccgacg cgggtgttaa cctgcgtctg accgatccct
    11881 tcaatcatgt attcgatggg atcttcaacc gtgagaatgt tgcgcgaatg gttgttcagc
    11941 tcctgcaatc cggcgtacag cgtggtgctt ttgccggaac ccgtcggccc cgtcaccaga
    12001 aagatgccgt gcggtttgtg taacagctgg cgcaactgcg cggtcagttg ctggctaagc
    12061 cccagacgct ccagcgtcag gcgggcctgg tttttgtcca gcagtcgcag caccacgcgc
    12121 tcgccccagg cagaaggcat ggtggagaca cgcacgtcaa tcgcccggcc gcccagcaac
    12181 agcgcaatac gtccatcctg cggcacgcgc ttttcggcaa tgtccagccg cgccatcacc
    12241 ttgatacgcg acaccagcag cgaggccagt ttgcgccccg gacgcagcat ttcatgtaat
    12301 gtgccgtcaa cacgaaaacg gatcaccaga ctcttttcaa acgtctcaat gtggatatcc
    12361 gaagcgcctt ctttgattgc ctctgccagc atggcgttga tcagtttgat gatcggcgca
    12421 tcgtcgtcgc tttccagcag atcttccgtt tccggcagtt cttccgcgag ggtaaaaaag
    12481 tcctcggcag aaccgagatc ttccatcagt tggcgagcct cggaagagtc ccgctgccag
    12541 accgcattca gccgctgttc aaattcggct tcgtcgattt gccgcagcgt aaagggcgcg
    12601 ttcagccccc gctgcagctc ctgcaaaaca gagagcgaca acggatgaac atggaggatc
    12661 tccagcgacg cttcgcacca tgccaccagg ctaaaccgac ggctgaaact gtagggcaga
    12721 cgcacggtgt tagcggtggt ttcctgtgct acaggcacca ttaacgcgtt ctcccggcat
    12781 taaggaacgc acgaacttcc ggcggtagag cctgattttg cgctggcagt accggctgcg
    12841 cggtgtgcgg catcaggctt aaaccttgct catcacgata gatctgttcg gcgcgcatat
    12901 agttatattt gcgctgcgac acgccgtctg ccgccatacc gtcacgcaga atggtcgggc
    12961 ggataaacac catcaggtta cgtttttctt ttttatccgc cgtcgattta aacaggttac
    13021 caatcaatgg gatatcgccc agcagcggca ctttcgccac gctttctccc gcctgatcgt
    13081 ccatcagacc accaagcaca atcagctcac catcgttagc cagcacggtg gttttcagtt
    13141 tgcgctcacc aaacaccacg tcgaggctgg tctgtccttc caccttcgag acttcctgct
    13201 caatcaccat ctgtaccgcg ttaccttcgt taatctgcgg cgtgactttc agcatgatgc
    13261 cgactttttt cctttctact gtattgaaag gattgctgtt attggagcca acggtagagc
    13321 cggttaatac cggaacgtcc tggcccacca tgaagaaggc ttcctggttg tccagcgtgg
    13381 tgatgctcgg cgtggagagc acgttcgagc tggagtcgtt tttaaccgcc tgcaccagcg
    13441 ccatccagtc gcctttcacc acgccaaccg ccgtaccgct gaatccagaa agaagctggg
    13501 cgagtgtgga gagatcgccg ttggtatccg gattaatggt ggtagcgccg ttttcactga
    13561 tcaccgtgga gcctttctgc ggtttcgcct gagaaatggc tgcccccagc gtgccaatag
    13621 ggatctgcgt accgttagca aactgcatta atccggcatc tttcgacgcc cactgcacgc
    13681 cgaaattgat attgctacct tcggcaactt ccacgatcaa cgcctcgacg tgcacctgag
    13741 cacggcgaat atccagttgt tcaatcacgc tttgcagcga ctgcatgatg tcctgcggcg
    13801 cagtaacaat cagggcatta ctgtgtttgc tggcggcgat ggagacaacc tcacgcccgc
    13861 taccgaccgt gccttccgcc tcttctttag ccgccgtgag cgtgccgctg acctgcttca
    13921 gcacatcgac cagatcttcg gctttgctgt atttgagata gaaaacctgg ctgttgccgc
    13981 tgcgttccat ttctgagtcc agccgacgga tcaggcggcg cattttgtcc cgcgtggccg
    14041 ggtcaccact gacaatcaca ctgttggtgc gttcgtcggc gacaatttga gatttcagcg
    14101 tcgcaggctg gttctcgccg ctgtttttcg tcaggctttc cagcacgcgg gcgatttccg
    14161 aagccgaggc gttatccagc gggatcacct cttcagtgcg attacccgcg tgatccacac
    14221 gctggatcac ttccgtcagc cgctccacaa cggaggcgcg tccggtgagc ataatcacgt
    14281 tggagggatc gtaattaaca acgttgcctg agcctgcgct gtcgatcatc tggcgcagaa
    14341 tcggtgccag ctcgcgtacc gacacattac gtaccggcac tactttggtg accatttcat
    14401 cgcccgcgta gttgtcgctg ccttcaccga ccagcggcag cggctcgact ttcgcagcgc
    14461 tggatttcac caccttcagc acgtcgtttt ccatcggtac gacggcatac ccctgtgctt
    14521 ccagcaggtt aaggaatagc tggtaatact ggcgttcatt gagcggggtc atggtgcgaa
    14581 tactcacttt cccctgtacg ccctgcccca taatgatggt tttattaagg ttagcgccga
    14641 cggtttcgat gaacgatttc aggtcggtat ctttaaaatt agcggtgaaa gtggcttctt
    14701 ccgcccagac cggtgaactg cataatgccg ctgccagcac cagcggcagt aaacgctttt
    14761 ttgttttgag gccagttgtc ttcttacgcc agaccgacaa cgtaatatca cgccaaaaca
    14821 cgatgaatga ttctcctgga ttaaatgcgg ttagcgcagc gcgatggaaa tgtcgtggcg
    14881 cgcacccttg cgtaaaaccg taagttgaat ggaatccatt gaaggtaact gccgcatcag
    14941 agcaatcatt gctcgtggat cagtgaaatc ctgctgattt agcgcaatgg cgatatcgcc
    15001 ttccttgaaa ccgctggcat cgaacagaga acgatctgcc cccggcttca ctgcataacc
    15061 gacaattccc tccttacgca caggcgtaag ctggatatag ttaaaaattt tctgcggatc
    15121 tttcgccagt gcctgacgca cggcagtcgg tatctcaact ggcgcactga cagcaggttc
    15181 agcaacagct tgctttgctt cgtcactgac agcttttttg ttggtcgcgg caacggtgga
    15241 acgctcctct tcagccaggc tcaggcgctc tatttttccc tgatagcgca gcatcacatg
    15301 gtcgcgattg atttcctcaa tcaccgcatt gtgcgagcca agcgtttcac cctgcaaata
    15361 gacctgctgt ttaccgcctt cttcaataac cgcgccgggt ctggcaccaa aggcaatccc
    15421 gcgcagcacc acattaagac gcgtttctgc cacaggcaca ggttcgggtt gttttaccgg
    15481 cgtggcgacg ggctgatatt tgccaaacca gttttgctgg ctgattaatt gcacgtcatt
    15541 tttatcgaac gtttttgcat ccgcacgggg cggtttattc gccgatgggg aaacagccgt
    15601 atattccgca gaaaaggaga aatagcgcca gagtgaatgc gccacttttg cagaaataat
    15661 aagcagcatc agccagaaca tcccgcgtac aatttttcgc agatgctctt tgtctgtatt
    15721 aaaagattgt ctctggttaa gagtgtgacg aatttttgtc agccattgaa tgagataaat
    15781 tcgtgcgtca cgaaaaacaa cccgcgccaa tttatatcta cccgacgtta tgctttgact
    15841 attccacagg tggtacgatc cagtttccgc tgatactggt tacctgtacg tgtatcatta
    15901 atcgttatgc tgtaattaat tccctcggta atcattaatt tcaccgttgg gtcagcgcac
    15961 atttgtcgtt gatagctggt taaaaaggcg tcaggcgtct gcgtggtctg agtccccgct
    16021 tcgctgataa tggtcatttt taccgtcgtg ccgctacttt gtgccagcac taaggtatat
    16081 cccgcagatt taatcggcag gttttggctg atattttgcg cctgtttttt cgccagtaaa
    16141 ctggcatttt cattatggct ggcacagcca cttaataatc ctgtaacgct caacaatagc
    16201 gatattaata ccctccctgg catttgtttt atcgacatgg taaataatct ctaaggttat
    16261 taataagagt taaaatgtca ctttgataat gacgttgtta tcattaaaac aatgcctgta
    16321 gataaattgt tgctataccg cccagactta aacacggtcc aaaaggcagt gtggttgatc
    16381 ctctttttgt aataacggca tatatcaggc cgcagcatga tgcgattaaa gcaacattgg
    16441 gtagcgacaa cggccccacc caactaccta acgcagcgaa caataataca tcgcccatgc
    16501 ctaatgcttc tttacgcaga actattccgg ctatccagcg cagagagtaa aaagtgataa
    16561 accccaccag gacgccggtg actgcatctt gtagcgtgag cgggctctgc tgcgcccatg
    16621 ccgcaatcag tcccgtccac aatacgccct gagtaaaaac atcgggcagc cattggtgat
    16681 cgaggtcaat gacgctcgcg gcaatcagcc aggcggataa tatcatcacc gccagcgccc
    16741 atccactttc cggccagacc agactcgcca gcaaaaaagc gagtgccgtc aataactcca
    16801 ccagcggata acgcttgctg attttcgcct gacaatcacg gcagcgccct ttgagcatca
    16861 gccaggagag cagcggaata ttgtcacgta tccggatggt ctgctgacaa tgtgggcagt
    16921 gggaacgcgg tagcgcaagg cttatttttg actgcgcact cggcatttca ccatgaaact
    16981 ccgccatttg ttggcgcagc atgatggggt aacgccaaat caccacattc aaaaaactgc
    17041 cgatgatcaa tcctccgacg gttgccagta tgggcatcgc cgcggggtat tgctgaaaaa
    17101 catcaaaaag catggttaaa ggttgtttgt tgtaacttgc cggatgcggg cctgcaagtg
    17161 tatgccatac ggctttcctc caggcccgat aagctggcgc aacccggtgc gccttatttc
    17221 atgccggatg cggcgtgaac gccttatccg gcatacagga ttactcgaca gacatcttat
    17281 gctcggtaac cttgttaatg gtttccggtc cctgttccgg tttcggcagc ttgagtgacg
    17341 ccagcgtgtt gtacgccgac tggctcacac cgccctcgaa gctcatctcg ctcgcccccg
    17401 gcagctggta agcattcgca cccggattcc atttcttaaa gaactccgaa agatccgtct
    17461 gggcgaccca ggaggcacac agcatcagcg tgtccgcagc gttaccgttg gattcagcac
    17521 agtaattctt gccaccaaag gtcttgtcac caacgtcatc gccgcgcgct ttacggtgca
    17581 tcaactggaa caggttccag cctttcatcc cttcacgatc gctgaagaac ttcggcaatt
    17641 caccttctgg ataccatttc ttgatatcaa agtttttctc tgcccattcc ttcagctgcg
    17701 cgtacatcag cagacggtca cccgcaccgc cgcgcgccca tgcctgaccg ttgctctcct
    17761 ccagatattc cggtgcgacg gtaatatcgt cagcgacacg gttcatcttg ccgagataac
    17821 gatcctgcat gtacagcgcc agcacgttgt tcgccacttc ggtcgcgcct ggaacagtca
    17881 gcggcgtttc tgcggcgttg tgccctactt cgtgccagat cagccagtcg ttcagcggcg
    17941 tcgtcggcag cgtggtgctg ttcgtcgaga agctgctgtt cattaccgga taacccgaat
    18001 gcgcatcacc gatggagatc tgcacatcgt tggcgaaacg atgcttgtgg cccgtcaagt
    18061 ttttataggt aaacatccgg tgcttaccgt cttcctcatt acgaccgtag aaatcattca
    18121 tcgagctggc aaaggtatcc agatcgttag cgaattgctt cagtccgcca gtgtaattgc
    18181 tggcattcag gttcttcttc ggtgcggtat aaacgaaagc gtctgattcc agctcaccca
    18241 gcggagccgg tgagttcaga tcgtttttcc atgcgccgtc tttatagaac ggtgctttta
    18301 ccacaccagt aaaggtgaag ctggcagatt cattgtcttt gctgtcgcct ttgatataaa
    18361 tcagaccacc gtaaggcacc ttgaatttca ccgtaccact agcgtccaga gagtacgttt
    18421 tggttactct tggcggacgg ttcagcgcga cttcatgctt ctctcttccg gtcaggtcgt
    18481 cagccagcgc cacggtgacg gtcacaggaa cgttcgcatt ggacttaatg gtgacctctt
    18541 tctgagccgg tgcccacagg ccagttgact gcatgttacc tgcaaaccat ttggtcggat
    18601 tcgagtacag gctgatggtt tcagtaacgt tctgtccctc ttccgatact gctcccggat
    18661 acttctcgac atcaactttg atgttcagat cccaccagga acggcccagc atcaggcgtg
    18721 tcagcggttt ttccatatag ttgagcgggt agctcgggtt catcatgccc gctttgctgc
    18781 tttcttcacc gtaaatcatc ttgttatcga tcagcgattt tttcagctca gcggaacact
    18841 gcgtgccaac gtatgcattg ttggtgtagc agttcagaaa ctcagtgaac gttttaaagc
    18901 ccagctcgtc ttctttaccc tcttcataac gatatttcgt ctcgttccac agccagacgg
    18961 acatgttttg gtataaacgt tccagatcga cgctgttgag acgctcaccc tggaggccat
    19021 tggtacggaa ataaagctca tgctgataca ggcgctgaat attggtgcct aaatccgcag
    19081 cctgcagcat cgctttcgca gtgtcagctc caagatccag ctgcgtatac tgcggaacat
    19141 acatgccacc ggttaccggc acacccgtgc ctgggcggta ttccagacag ttgacctcat
    19201 agtgatagtc agaatcctta cacacttcca gccctgggaa cgcgtccaga attctctgtt
    19261 ttgccgcagc cagtgactca ggcgttttgt ggtcggcttc atcaataaag gcgaattggg
    19321 ttacctgttt accctcaact tcttcctgcc agctggcaac ttccagcttc ggtttgtcat
    19381 caggtttgtt ttcttgctga tatttccaga taacttcttt cgtggtgtca tcaatggtat
    19441 acggtggttt accatcgaca gccggataac gctcatagac ccaaattggc gttgaacgtc
    19501 gttgacgaac acggtcaggg tagccttgcg gatcgttatt tactaccgac ttgttaagcg
    19561 ccatcgacaa acctgcggca tcaagcagac gtacaaagcc agatgcgctc tcttccttaa
    19621 gattgctcat cacgttttcc atgatcagca ccgatccacc tttgttcata taggcgatca
    19681 aatcggtcac atcctgctgg gtcagcttcg gtttgctggt atctgcgcgc agaggaatgg
    19741 agtaaggatc gctaccccat tgtgtgacgt attcaaagcc attgaggatc aacagaggaa
    19801 cttcatctgg attcagattg ccatagctgg tcattggttt gaccgtgatg ccagtgaaat
    19861 ccttgtggaa cgcaaacggt gcgctatttc ccagcacctg accatgtttt ttgaaatata
    19921 ccgtgtccag gttagtaccc acggtcatac tggattttgc atccggcaac cagcgatcat
    19981 ttgacagata gcgcagcacg ttctccatga agttcttcat gtcatccggg tcgctgttga
    20041 gcgtacactg tccgtcttta ttaacgcccc cgttccagct gtagccgttc gggcaacgca
    20101 ggatgctgtt gtagtgtggg ttaccgataa ccatcagctt gccatcgccc acttgcccca
    20161 gcgagataaa cggcaggttg aaggtggcgg tttcgcgtgt cacgttctct ggtcgcacaa
    20221 tggaaggcgc ttcagtaatg tacgccagct catttttatc ccaggcgcgt ttttcgccaa
    20281 acgccagcca gtagttttta tcattacgcg ccatcagaat cgggaaggcc gcgttggaga
    20341 tattcaccac cgcctgaccg cgcgcattac ccgtgctgcc atagaagttg gtagagtcat
    20401 ggaatacatg gaacttactg acagatttgt agttcgtatc cacgccccac agcttgttga
    20461 taacgccctg aatctggccg tcattaacat tgcgcgttgt cagcgagaac cagcgagcct
    20521 cgttacaacc atcggttttc gcacaaatcg cggtatcgat ctctttggcc tgacccgtct
    20581 taaactgctc gataaattcg ttaggcagat taacgacttg ctcaccttcc cccagcgtcg
    20641 caccattgga taacgagaga ttgataatct cgttaatcac gttgggatat tcagcaaaaa
    20701 ccttgcgcac ttcatccgga actacacgcg tgtgattttg tccagccttc gaatagcggt
    20761 gaatcaactg atcgatattt gccccgcgaa cttcatcacc cagttcagtc aatgcaatag
    20821 tcgacttgtt accacgcaca gaaccaagct caaaagtgtc gatgccaaag gagatggttt
    20881 ccccccaact aaaggaaaat tccccgtttt ctccggttac accacggccg gaattggtgt
    20941 aatagttgac accaacaaca ccatccccct gactatcgac cagtcgacct tcagagagaa
    21001 tgatttcagt gggttgatac tgataaaact gttccgcgtt agccgacacg aaggaagcgt
    21061 ttagatccgg ttttgttccc ggagtggtgg cgggcacgac cggtgaagta tgagtggatg
    21121 gcgctttatc ggtcgcggca ttgttttcca cctcttcatt gaccagcttt ttgaattctt
    21181 ccggtgccag atcgatttgc ttatacagcg agtcgaaacg tttactctcg atcaccgagg
    21241 agaactccag gcacacctgt tctgtattcg ccgggcaact gttcatggag gtgaccagcg
    21301 agagcgcatt gcttttcttg ttgtcggaac ccgccagttc ttgcgcgtcc tcaagactaa
    21361 acgacacttt ttcaaccgca cgcaggctac gcgcagcttc tgactgggtg tcgaaggtag
    21421 caattgtcgt gttgttccct gccacacagg tgactttgtc gcctggtgta aaggtaaagc
    21481 catcgctgga ttcatcatta caggtgatat caccagttac ccgcaggctt ccgcccagag
    21541 tcagataacc cgttttcgta ggaacaggtt ctggctccgg ttctggtgtt ggctcaggat
    21601 caggtgtcgg ttccggcgta ggttctgggt ccggcgttgg ctcaggtgtc ggctccgggt
    21661 ttggtgttgg atcaggtttc acttccggca aagaccctgt tccagaatct acaggcggcg
    21721 tatcggagga agatccggag ccaccgccat cacaaccggc taacagggtt gcactcaaaa
    21781 tagccgctaa aagcgatt
//
LOCUS       DAZFRN010000077        21751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFRN010000077 DAZFRN010000000
VERSION     DAZFRN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.077"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            238..543
                     /gene="gfcA"
                     /locus_tag="ACHKUB_002813"
     CDS             238..543
                     /gene="gfcA"
                     /locus_tag="ACHKUB_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO6655798.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            650..1294
                     /gene="gfcB"
                     /locus_tag="ACHKUB_002814"
     CDS             650..1294
                     /gene="gfcB"
                     /locus_tag="ACHKUB_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO6655799.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1291..2037
                     /gene="gfcC"
                     /locus_tag="ACHKUB_002815"
     CDS             1291..2037
                     /gene="gfcC"
                     /locus_tag="ACHKUB_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6655800.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2037..4133
                     /gene="gfcD"
                     /locus_tag="ACHKUB_002816"
     CDS             2037..4133
                     /gene="gfcD"
                     /locus_tag="ACHKUB_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6655801.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4179..5318
                     /gene="gfcE"
                     /locus_tag="ACHKUB_002817"
     CDS             4179..5318
                     /gene="gfcE"
                     /locus_tag="ACHKUB_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO6655802.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5306..5752
                     /gene="etp"
                     /locus_tag="ACHKUB_002818"
                     /gene_synonym="yccY"
     CDS             5306..5752
                     /gene="etp"
                     /locus_tag="ACHKUB_002818"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO6655803.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5772..7952
                     /gene="etk"
                     /locus_tag="ACHKUB_002819"
     CDS             5772..7952
                     /gene="etk"
                     /locus_tag="ACHKUB_002819"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO6655804.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8067..9365)
                     /gene="appA"
                     /locus_tag="ACHKUB_002820"
     CDS             complement(8067..9365)
                     /gene="appA"
                     /locus_tag="ACHKUB_002820"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO6655805.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFQQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9445..9537)
                     /gene="cbdX"
                     /locus_tag="ACHKUB_002821"
     CDS             complement(9445..9537)
                     /gene="cbdX"
                     /locus_tag="ACHKUB_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO6655806.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9550..10686)
                     /gene="appB"
                     /locus_tag="ACHKUB_002822"
     CDS             complement(9550..10686)
                     /gene="appB"
                     /locus_tag="ACHKUB_002822"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6655807.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10698..12242)
                     /gene="appC"
                     /locus_tag="ACHKUB_002823"
     CDS             complement(10698..12242)
                     /gene="appC"
                     /locus_tag="ACHKUB_002823"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO6655808.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12376..13233)
                     /gene="hyaF"
                     /locus_tag="ACHKUB_002824"
     CDS             complement(12376..13233)
                     /gene="hyaF"
                     /locus_tag="ACHKUB_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO6655809.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13230..13628)
                     /gene="hyaE"
                     /locus_tag="ACHKUB_002825"
     CDS             complement(13230..13628)
                     /gene="hyaE"
                     /locus_tag="ACHKUB_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO6655810.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13625..14212)
                     /gene="hyaD"
                     /locus_tag="ACHKUB_002826"
     CDS             complement(13625..14212)
                     /gene="hyaD"
                     /locus_tag="ACHKUB_002826"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO6655811.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14209..14916)
                     /gene="hyaC"
                     /locus_tag="ACHKUB_002827"
     CDS             complement(14209..14916)
                     /gene="hyaC"
                     /locus_tag="ACHKUB_002827"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO6655812.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14935..16728)
                     /gene="hyaB"
                     /locus_tag="ACHKUB_002828"
     CDS             complement(14935..16728)
                     /gene="hyaB"
                     /locus_tag="ACHKUB_002828"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO6655813.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16725..17843)
                     /gene="hyaA"
                     /locus_tag="ACHKUB_002829"
     CDS             complement(16725..17843)
                     /gene="hyaA"
                     /locus_tag="ACHKUB_002829"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO6655814.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18270..18357
                     /locus_tag="ACHKUB_002830"
     tRNA            18270..18357
                     /locus_tag="ACHKUB_002830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18304..18306,aa:Ser,seq:tga)
     gene            18498..18827
                     /locus_tag="ACHKUB_002831"
     CDS             18498..18827
                     /locus_tag="ACHKUB_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6655815.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
     gene            complement(18991..19662)
                     /locus_tag="ACHKUB_002832"
     CDS             complement(18991..19662)
                     /locus_tag="ACHKUB_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO6655816.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPNTPENRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            complement(19662..19886)
                     /locus_tag="ACHKUB_002833"
     CDS             complement(19662..19886)
                     /locus_tag="ACHKUB_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6655817.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            complement(19896..21296)
                     /locus_tag="ACHKUB_002834"
     CDS             complement(19896..21296)
                     /locus_tag="ACHKUB_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6655818.1"
                     /translation="MTVRISGVLKDGTGKAVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKYLMDLEDAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERG
                     PKGERGETGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAAT
                     PKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTETGEWTTSEFVA
                     WLKEKGAFDQPYWMMKASLLAEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVRVTIG
                     EYGGDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLRQW
                     GSIEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPNVIKMYTLSNPSLVSVTLI
                     TVRGCYGKFSWEAIGY"
     gene            complement(21439..21579)
                     /locus_tag="ACHKUB_002835"
     CDS             complement(21439..21579)
                     /locus_tag="ACHKUB_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6655819.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHTSLCEFRFRIAGAEIAAFLQC
                     KQ"
BASE COUNT         5191 a         5960 c         5498 g         5102 t
ORIGIN      
        1 taacaatttc cttatcttta atttgtattt tgctaagatt tatcttatgc tcacaagtcg
       61 tatttccaga gggggatagt taacaatctt atatttccct ctgcgcgcct ttcctacccg
      121 cagtatatag tgcaagcggt atgaggaaag ggagatttca caaggatgtt atactcaaag
      181 tagacaatcc tgatctcaag cgtacgtatt gtccatgcga tgaataaagg aaaagttatg
      241 aaacacaagc tttctgcaat ccttatggcc ttcatgttga cgacgccagc tgcgtttgct
      301 gcccctgaag caacaaacgg gaccgaggca acgacgggta ctactggcac cacaacgacc
      361 actaccggcg caaccacgac tgctactacc actggtggcg tggctgctgg cgctgtcggc
      421 acggcgacag ttgtcggtgt ggcaacggct gttggtgtgg cgacgttggc agttgttgca
      481 gcaaatgaca gtggcgatgg cggttctcat aacacctcaa ccaccacgtc cactacacgc
      541 taatactgct ttaacgcgtc gaaattaaag ttaaatgata cccggttgcg atattcgtac
      601 cgggctttag agatttgcat ttttttaatt aacctttcaa ggattaaagg tgcgccctct
      661 tattttatcg attttcgcac tatttcttgc gggatgtacg cacagccagc aaagtatggt
      721 cgatacattt cgcgccagcc ttttcgataa tcaggatatc accgtagcgg atcagcagat
      781 ccaggcgttg ccttattcca cgatgtattt acgccttaat gaagggcaac gaatctttgt
      841 ggtactggga tatatagaac aagaacaaag caaatggtta tcccaggata acgccatgct
      901 ggttacccac aatggacgtc ttttaaaaac cgtcaaactt aataataatc tgctggaagt
      961 gacaaattcc gggcaggacc ctctgcgtaa cgcgctggca ataaaagatg gcagccgctg
     1021 gacgcgcgat attctctgga gtgaagacaa ccattttcgc tctgcgaccc tgagttctac
     1081 tttttccttt gctggattag agacgctgaa tattgcgggt cgcaatgtgc tgtgtaatgt
     1141 ctggcaggaa gaggtgactt ccacgcggcc agaaaaacag tggcaaaaca cattctgggt
     1201 cgattcggct actggccagg ttcgtcaaag tcggcaaatg ttaggcgcag gggttattcc
     1261 cgtagaaatg acgtttctta aacccgcacc atgaataaat tacagtcgta tttcattgcc
     1321 agcgtacttt acgtaatgac accccatgcc tttgcgcaag gaacggtgac tatttatctg
     1381 cctggcgaac aacagacact ttccgttggc cccatggaaa atgttgtcca gttggtgaca
     1441 caaccgcaac tgcgcgatcg cctctggtgg cctggtgcct tgctgaccga ctctgcggcg
     1501 aaagctaaag cgcttaaaga ctaccaacat gtcatggcac agcttgcttc ctgggaagca
     1561 gaagctgacg acgatgtggc ggcaacgatt aaatccgtgc gccagcagtt actcaatctc
     1621 aatatcactg gtcgcttacc ggtcaaactg gaccctgatt ttgtacgagt ggatgagaac
     1681 agcaatcccc cgttggtcgg ggactatacg ctctataccg tacagcgtcc ggtgactatt
     1741 actctgctgg gtgcggtttc tggggccggg caattgccct ggcaggcagg ccgtagcgtt
     1801 accgattatc tgcaagatca cccccgcctt gcaggcgcgg acaagaataa cgttatggtg
     1861 attacgcctg agggtgagac tgtcgtcgcc cctgttgccc tgtggaataa aagacatgtg
     1921 gaaccaccgc ctggcagcca gctttggttg ggcttttctg cgcatgtact gcccgaaaaa
     1981 tatgctgatt taaatgacca gattgtgtct gttttaacgc aacgtgttcc tgactgatga
     2041 agaagaattc ttatctttta agctgcctgg ccattgccgt ctccagtgcc tgtcatgctg
     2101 aagtattaac ctacccggat ccgctgggtt cgtcgcaatc agactttggc ggcacaggat
     2161 tgttgcagat gccaaatgcg cgcatcgcac cggaaggtga attcagcgtc aactaccggg
     2221 ataacgatca ataccggttc tactccacct ccgtggcgct gttcccatgg ctggaaggca
     2281 ccattcgtta tacggatgtg cgcacacgca aatatagcca gtgggaagat ttcagcggcg
     2341 atcagtcata caaagacaaa tcattcgatt ttaaacttcg cctgtgggaa gaaggttact
     2401 ggctaccgca agtggcgttt ggtaaacgtg atattgctgg tacgggtctg tttgacggtg
     2461 agtatctggt ggccagcaag caagcggggc catttgattt caccctcggg atggcatggg
     2521 gctacgccgg taatgcgggc aatattacca acccgttttg ccgggtgagc gataaatatt
     2581 gtcatcgcgc agagtctcac gatgcgggcg atatcagctt tagcgatatc tttcgtggcc
     2641 cggcttccat ctttggcggc attgagtatc aaacgccgtg gaatcccctg cgtctgaaac
     2701 tcgaatacga tggcaacaat taccagaatg atttcgctgg caaactgcct caggcaagcc
     2761 atttcaacgt cggcgcagtt tatcgcgctg ccagctgggc agatctcaac ctgagttatg
     2821 aacgcggtaa cacgttgatg tttggcttca cgttacggac caatttcaac gatctgcgcc
     2881 ctgccctgcg cgatacgcca aaaccggcat atcaacctgc gcctgaatct gaaggattgc
     2941 agtacaccac agtagcgaac caacttaccg cgctgaagta caacgcaggt tttgaagcac
     3001 cggaaattca gctgcgcgat aagacgctgt atatgtctgg tcaacaatac aaataccgtg
     3061 attctcgcga agcggtcgat cgtgccaacc ggattctggt gaataacctg ccgcaaggcg
     3121 ttgagaagat tagcgtgacg caaaagcgcg agcatatggc gatggtgact accgaaaccg
     3181 acgtagccag cctgcgcaaa cagctggctg gtacagcgcc tggtcaatca gagcaacttc
     3241 aacaacaacg tgttgaagca gaagatcttt ctgcctttgg tcggggctac cgtattcgtg
     3301 aagatcgctt tagctactct ttcaacccaa cactttcaca gtcgctgggc ggcccggaag
     3361 atttctatat gttccagctg gggctgatgt ccagtgcccg ctactggttt accgaccacc
     3421 tgctgcttga tggcggtatt ttcaccaata tttacaacaa ctacgacaag tttaagtctt
     3481 cgctgttgcc cgcggactct accctgcccc gcgtgcgcac gcatatccgt gactacgttc
     3541 gcaatgacgt ttatctcaac aacttgcagg cgaactattt tgctgactta ggcaatggtt
     3601 tctatggcca ggtgtatggc ggttatctgg aaacgatgta tgccggtgtc ggttccgagc
     3661 tgctttatcg cccgctagat gccagctggg cgctgggtgt ggacgttaac tacgtgaagc
     3721 agcgtgactg ggacaacatg atgcgtttca ccgattattc cacgccaaca ggtttcgtga
     3781 cggcttactg gaacccgccg acgctcaatg gcgtactgat gaaacttagc gttgggcaat
     3841 atctggcaaa agataaaggc gcaacgatcg acgtcgccaa acgctttgac agcggcgtgg
     3901 cggtaggggt atgggcggca atcagtaacg tatctaaaga tgactacggc gaaggcggct
     3961 ttagtaaagg tttttatatc tcgattccat tcgacttgat gaccattgga cctaaccgca
     4021 accgcgcggt ggtttcgtgg acaccattga cgcgtgatgg tggacaaatg ctgtcacgca
     4081 aataccagct ctatccaatg acggcagagc gagaagtacc ggttggacaa taacctgacg
     4141 taattcataa cattacattg actgataaat ataaaccaat gaaaaagaac atttttaagt
     4201 tttcggtact gacattggct gttctgtctt tgacagcatg tacccttgtt cctggtcaga
     4261 atttatcgac cagtaacaag gatgtcattg aactacctga caatcagtat gacctggaca
     4321 aaatggtcaa tatctatccc gtcacgccag gattgattga tcagttgcgc gccaaaccga
     4381 ttatgtcgca ggctaacccg gagctggagc agcaaatcgc aaactatgaa tatcgtatcg
     4441 gcattgggga tgtgttgatg gttacggtct gggatcaccc ggaactaacc acccccgcag
     4501 ggcaataccg tagcgccagt gataccggca actgggttaa cgccgatggt gcgattttct
     4561 acccctatat tggtcgcctg aaagtggccg ggaaaacact tacccaggta cgcaacgaga
     4621 ttacagcgcg tctcgattca gtcattgaaa gcccgcaagt cgatgtcagc gtcgcggcgt
     4681 tccgatcgca aaaagcgtac gtcaccggcg aagtcagcaa atcgggtcag cagccgatta
     4741 ccaatattcc tctgaccatc atggatgcga ttaacgccgc gggcggtctg accgcagatg
     4801 cagactggcg caacgtggtg ctcacccaga atggcgtaaa gaccaaagtg aacctctatg
     4861 cgctgatgca gcgtggcgac ttgcgccaga acaaactgct gcacccgggc gatattttat
     4921 ttattccgcg caacgatgat ctgaaagtct tcgtgatggg tgaagtgggt aaacagagca
     4981 cgctgaaaat ggaccgcagc ggtatgaccc tggcggaagc gctgggtaac gctgagggta
     5041 tgaatcagga tgtcgctgat gcgacgggga tttttgttat tcgtgcaacg caaaacaagc
     5101 agaatggcaa aatcgccaat atttatcagc tgaatgcgaa ggacgcatcg gcaatgatac
     5161 tcggaacgga attccagctt gaaccttacg acattgttta tgtcactacc gcgccgctgg
     5221 ctcgttggaa ccgtgtgatt tctctgctgg taccgacgat ttccggggtg catgacctga
     5281 cagagacttc acggtggatt caaacatggc ccaactaaaa tttaactcaa tcctggtggt
     5341 ttgtaccggc aacatttgcc gttcgcccat tggcgagcgc ttgctgcgta aacgtctgcc
     5401 gggcgtaaaa gtcaaatcgg caggtgttca tggtctggta aaacaccctg cagatgcgac
     5461 agcggcagat gttgccgcta atcatggcgt atctcttgaa ggacatgccg gacgtaagct
     5521 cactgctgag atggcacgaa attacgatct gattctggca atggagtcgg aacatattgc
     5581 tcaggttacc gcaattgctc ccgaggtccg cgggaagaca atgctgtttg ggcaatggct
     5641 ggaacagaaa gagatcccgg atccctatcg taaaagtcag gacgcatttg aacatgtcta
     5701 cggtatgttg gagcgcgcca gtcaggaatg ggcgaagcgc ctcagccggt aatttgagtt
     5761 tataaaatac gatgacaact aaaaatatga atacgccacc aggcagcact caggaaaatg
     5821 agatcgatct gcttcgtctg gtcggcgagt tatgggatca ccgtaagttt attatcagcg
     5881 tgaccgcgtt attcacgctg atcgctgtcg cttactcgct gttaagtaca ccaatttatc
     5941 aggcagatac tctggtccag gttgagcaaa aacagggcaa cgccattctc agcggcctga
     6001 gcgatatgat ccctaactca tcgcccgagt ctgcaccgga gatccaactg ctgcaatcgc
     6061 gcatgattct cggtaaaacc attgctgaac tgaatctgcg cgacatagtt gagcagaagt
     6121 attttccgat tgtgggtcgc ggctgggcga gattaaccaa agaaaaacca ggtgagctgg
     6181 cgatcagctg gatgcatatt ccacaactga atggtcagga tcagcaactg acactcacgg
     6241 ttggggaaaa cggccactat acactggaag gtgaagagtt caccgtcaat ggtatggtcg
     6301 gccagcgtct ggaaaaagat ggcgttgcgc tgactatcgc ggacattaag gccaaaccag
     6361 gaacacagtt tgtcctgagc cagcgtaccg aactggaagc gattaacgca ttgcaggaaa
     6421 cctttaccgt tagcgaacgc agtaaagaaa gcgggatgct ggaacttacc atgactggtg
     6481 atgatcccca gttgattact cgtattctga acagcatcgc taacaactat ttgcaacaga
     6541 atatcgctcg ccaggcggcg caggattcac aaagccttga attcttacag cgccagttac
     6601 ctgaagtgcg cagcgagctg gaccaggcgg aagaaaaact caacgtttat cgccagcagc
     6661 gcgattcggt tgaccttaac ctggaagcca aagccgttct tgagcagatt gtgaacgttg
     6721 ataatcaact caatgagctg accttccgcg aggcagagat ctcccagctg tataagaaag
     6781 atcacccaac ttatcgtgcg ctgctggaaa aacgccagac gctggagcaa gaacgcaaac
     6841 gcctgaataa gcgggtatcg gcaatgcctt ccacccaaca ggaagtgttg cgtttaagtc
     6901 gtgacgtaga agcgggccgt gcggtatatc tgcaattact taaccgccag caggagttga
     6961 gtatttcgaa atccagtgcc attggtaacg tgcggattat cgacccggca gtcactcagc
     7021 cgcagccagt gaaaccgaaa aaagcgttga atgtggtgct tggttttatt cttggcctgt
     7081 ttatttctgt gggtgccgtg ctggcgcgtg cgatgttgcg tcgtggtgta gaagccccgg
     7141 aacaactgga agagcacggc atcagcgttt atgccactat cccaatgtcc gagtggctgg
     7201 ataaacgcac ccgtctgcgt aagaaaaatt tattttctaa tcagcagcgc catcgtacta
     7261 aaaatatccc cttcctggcg gtggataacc cggcggattc tgctgtggaa gccgtacgtg
     7321 cgctacgaac cagtctgcat ttcgctatga tggagacgga gaataacatt ctgatgatca
     7381 ccggtgcgac gccagacagt ggtaaaacgt ttgtcagttc aactctggca gcggtgatcg
     7441 cccagtccga tcaaaaagtg ttatttattg atgccgactt acgccgtggt tattcgcata
     7501 acctgtttac cgtgagtaat gaacatggct tgtcggaata tctggcaggt aaagatgagc
     7561 tcaacaaagt gatccagcat tttggcaaag gaggctttga tgtgattact cgcggtcagg
     7621 tgccacctaa cccgtctgaa ctgctgatgc gcgatcggat gcgtcaatta ctggaatggg
     7681 cgaacgacca ttacgatctg gtgattgtcg atacgccgcc gatgctggcg gtgagtgatg
     7741 ccgcggtcgt ggggcgttct gttggcacca gcctgctggt tgcgcgtttt ggcttgaaca
     7801 ccgccaaaga ggtgagtttg tcaatgcagc gtctggaaca ggcaggcgtc aatattaaag
     7861 gcgctatcct caatggtgtg attaaacgcg ccagcaccgc ttacagttac ggctataact
     7921 attacggtta tagttactcc gagaaagagt aaaactgaaa gtggccggat gcggcgtgaa
     7981 cgccttttcc ggtctacgca ttagacagtt cttcgtttgt catcagcctc agagcattca
     8041 ggtaactgaa tgctcttttt tatgcattac aaactgcacg ccggtatgcg tgcttcattc
     8101 acgatttgcg taaaacctgc caacgaacac atgccctgcg catttcgctc ttcacatcct
     8161 gccagggtca gtttcacctc tccgggcggc gtatttaatg acagcggcgt tttatcacgc
     8221 atctgctgta aagtctggaa gaccagcgaa acctgaatcc actggctgtt atcgcttagc
     8281 cgacgccagc gttcaaacac cagttcacca cctggcggcg tgttatccgg ctgaccggga
     8341 agcgtccagt tgagctccag tgcgccgccg agatttgcca gattagtatc gtgtccggca
     8401 ataaacagta ctgaagtggg taatgtcaca ccatacgcct gtttttgcgg tggatggggc
     8461 gtcaacgctg ccatgatcaa atccaataac ggggtggcgc ggctgcgggc aacctctggc
     8521 gtgcgttgta gcaaataaaa ttgcgcgtta tgcaaactta gcaaggtgtt ccactggtgt
     8581 gaatcggtga tccttcccca ccccggctcc ggcattccct gtgcttgttg caggagaaat
     8641 atctccgtca gcattgatgc gaggcttacc gcaccggtta atgagacatt gtcggcgctc
     8701 accttgagtt ccgatggtaa tgcctgcgtt aatgaacagc tttcgtcctg tttctcacgt
     8761 ttaaggcaca agtttgattg ctgaaaatta agcacccgtt ccagttcgcg aaacgccgtt
     8821 tgccgatgcc cggtaaagtc agcaattgac cctcctgccc tgctgaggat cgcgtcagtc
     8881 acgttcgcat tatccagttg gcaaacgcca gtttttagag gattaaataa cggatcggga
     8941 ctggacgtat ctgcctgggt atgtacggtt attgcacagt caggtgccag cccggcggcg
     9001 aaggcttcgc ctgttttacg ggtacgctcg tcgacatcag caataatcgc gacctgacca
     9061 gactgcgggc agcccttttt cgccagcaat ccgtcggcta ccagacgctg gcgttggtaa
     9121 tgtccgagat aggcgattag ctcaccaccg cgcggtgtca gccaacccag ttttaccggc
     9181 caggttggcc atgcgtctgg ggtgacatcc tgcatcagtt gcgtggcctt ggttggagca
     9241 cgcacaccat gacgactgac aatcaccaca ctttccagct tcagctccgg ctcactctga
     9301 gcgaatgcag attgcggggt taacggaatc agaagagata aaaatgggat taagatcgct
     9361 ttcatcgata tgtttccgct tttcatatct gacattattg attgcctgat gcgatgctaa
     9421 tgcgccgcat caggcctacg ttcgttaact tttcagacgc gggtccagcc ataccaacac
     9481 aagggtggag agcgaacaca tcaacaaaat gccgacgaac caaagtaaat accacattgt
     9541 tcctgctcct tagtacaact cgttttcgtt acggcggaga gtttctgttg tcatgcgtcc
     9601 ccacattttg tagtagctcc agagagtgta gagcaacaca atgggcaaaa atatcagcac
     9661 gattaccaac ataatgctca gcgtcagctg actggaagtg ctgtcccaca acgtcaggct
     9721 ggagattgga ctcacgcttg acggcatgac aaaggggaac agcgtgatgc ctgccgtgaa
     9781 aatcacgcca aattgcatca atgacgccat taaaaatccc caacccgggc gaccacgata
     9841 aatcgccatc accgtcagca atgggcagaa gaaccccagc agcgggaaga tccacaaaac
     9901 gggcgattcg acgaaattat tcatccaggc accaggtagc actgccacca gtttcattaa
     9961 cggattggaa ggaccgttag catcctgggc gagcagtaca aagccatcaa taccgaccca
    10021 cagccagtaa cccgccagca aaaagcacag catcaccaac agtgcagcgc gtttggtggc
    10081 cagctgtgaa cgcagatgaa tcacaccaac agttttcagt tgtaaccaga cgccaccttg
    10141 caaaatcacc atcccaaggc tgagcaatcc gcacaataaa gggaatggcg tcagcagttg
    10201 ccagaagctg ccgagatact ccacgcgtaa ttgcggtgtg aaggcaaacg gcacgccgag
    10261 caacaagttg ccgaaggcga taccgaagac taccggcggc accagactgc cgatgaccag
    10321 accggcatcc cacattttac gccagcgtgc atcggcaatt tttccgcgat aatcaaaggc
    10381 cagcggacgg aagaacagcg agcacagcac caggatcatc gccacataaa agccggaaaa
    10441 cgccgctgca tacactctgg gccaggcggc aaataatgcc ccaccagcga ggatcaacca
    10501 gacctggttg ccttcccagt gtgcaccaac gctgtttatc actatccggc gttcatcatc
    10561 attacgcgcc accagcggca gcagacagcc gatccccatg tcaaatccgt cggagatcat
    10621 aaagaccacc aggatcacgc caatcagcag ccaccagatg aagcgcaatg tttcataatc
    10681 aaacatgatt ttctccttta cccctgttgc tgcgtcggtt gttcactctg catcgcgctc
    10741 ggccccagac gggcatattt ctgcatcagg tagacttcgg cgattaagaa cagggtgtaa
    10801 agccctacga tcatgatcag tgagaaagcc agttgtcctg tggttaaagc ggagtgcgcg
    10861 gagtatgtcg gtaagatgtc ctgtatcgcc cacggctgac gaccaaactc ggtcataaac
    10921 cacccggctt caatcgcaat ccacggcaac ggcaaactcc agagcgccat tttcagcacc
    10981 cagcgatgct ggtcgatttt gccacgcagc gtctggacaa gcgcaatcag catcaccagt
    11041 agcagcaggg aaccacagcc caccatgatg cggaaactcc agaataccgg cgcaacctga
    11101 ggtatcgcgc cacgcatcgc cgcctggtac tgtgcggctg tgacatgatt catatccggc
    11161 gcatagcggg agagcaacat gccgtagccc aggtcacctt ccagttcccg gaatgcctgc
    11221 aacacatgcg gctcacgatt gccttgcgat atttcctgca ttaacagcca ggccatacgt
    11281 ccgcgttgca agcgtgggta ggtttcagcc atcaaattct tcagacccgg cacgggttta
    11341 tctaatgagt gagtggcgag gatccctagc agcgcgggaa ttttgatggc aaaggcgtta
    11401 cgctcttgat cctgttccgg ccaggcaacc acatggaacg gtgcaggtgc aggttccgtt
    11461 tgccactccc cttccatcgc cgccagtttt accggttgta cttgcgcgac ttcatacgca
    11521 gaactgtctc cgagttgcag ggtaccgata atcgccagag taccgaagac ggaaccgatg
    11581 gcaaacgaac gtaatgcgac atcgcgctcc cgtccgcgca gtaaatacca ggcgctgatc
    11641 gccataataa acatggcccc ggtcacgtag cccgccatta cggtgtgcac aaatttcacc
    11701 tggctgaccg ggttaaagac cagctcgctg aagctggtca tctccatacg cagggtgtcg
    11761 atatcaaaat gcgcaccggt cgggtattgc atccaaccgt tggcattcaa tatccacaac
    11821 gcagagagat ttgaaccgaa cgccaccagc cacgtcacca gcaggtgctg gtatttattc
    11881 agccgttgcc agccgaagaa gaacagcccg acaaaggtgg attcgaggaa gaaggccatt
    11941 aatgcttcca tcgccagtgg tgcgccaaaa atatcgccca catagttgga atagaatgac
    12001 cagttagtac caaactgaaa ctccatggtc aggccggtag ccacgccaag agcaaaattg
    12061 ataccgaaga gcttacccca gaagcgcgtc atatcgcggt agattgtttt gccggtgacc
    12121 acgtaaatgg tttccatgat agccagcaaa aaaatcagcc ccagggtaag gggtacaaat
    12181 aaaaagtgat acagcgcggt cagagcaaac tgccagcgcg ataaatcaat gacatcccac
    12241 atgcgcactc ctgtaggcga agagagaaaa ataacccgat aatggtagat ctccctcttt
    12301 atcctgaaac tctacgacca gaggtattcg ccgattgcga tattcataag actgaaagcg
    12361 atacttaccg tctttttacg tcggtgcagc ttccgccagc cactgacata cctcgctaag
    12421 ccgctgcgca gagtcgacca aatcttctgg cgctgccagc accacttccg gtatcgggca
    12481 gatttcataa ctttccagta aagggccttt taaggtgtcc gtacagcgta aatgccagac
    12541 atggcgtaac cccgtggcgt tgatatagct ctcgccatag ccaatggtac gaatctgaat
    12601 atttcccggc ccacagagac gtgagagaaa aagccgatca gcctcgctga tgggtaattg
    12661 cgtcagatta atgctgtgcg gctgcgcgtc ggggttacgt acatgtgcca gtaactcatg
    12721 cgccaacggt aggccattca tcaggccatc aatgggcggc ggtaacagcg aatctgtcgg
    12781 caagagattt tgcgttgccg cctgccacaa cgccagcggc gcacagccag cttccagttt
    12841 gtcctccagc aacttttcgc cgcgacgtct gcgcacccgc cataatccgc agaagatcgc
    12901 ctcctgtatt tcactttcac tgtcgtcagc ctgctgaatg cgcactgaca cttccccttc
    12961 cccgagaagg gtattgataa agtgacgatc gtcggcgtgg agatttaaca gatcgactgc
    13021 cagcacctcg cccaacggcg gctggcgttg actcatttga tgttgtaact cgtttaacag
    13081 cgcaatcacc tgcgggctgt gagcacattg ttccagggcc gccatactcg gttcatcatt
    13141 cacctgacag gtgatcggca gtggattcat gctgaaactg tcatcgttcg gttgcgttcc
    13201 tggccccagc agatggaaaa aagtttcgct catgaggcac gctcctgctg cggttcgaca
    13261 agcccgcgca tcaggtttat cagttccgcc cacgggtgaa taccattcag cacaccgcga
    13321 tagtttccgc cggtaaacac taatgtggcg ggaaagcgaa agacgccaaa gcgatcgccg
    13381 atggcttcgc tctgctcaag gtcagcaatc gccacctgcc atgtatagtc gggaaactcg
    13441 cgcagtaatt cgccaatcat taccggatta tcgctgacct ctggcgtacg tttcgggtca
    13501 ctgcttaata acaccacgcc gtctggcgct tgcgtaagcc agtcgtcaag acgggattca
    13561 ctgaccggcg tccagccgcg cgccagcatt cgttgccaca acgcatcaaa tggcgtgtcg
    13621 ttgctcatcc ctgctcctcc agtgtcatcc tgtactggcg caaacgaacg ccttcgtaat
    13681 tttccatcga cagacagtca taattgagac agcgcgattc attagccggt tgcggcacaa
    13741 ttccccacgc agccagttgc gccaacgccg attgttccgc agcgggcagt tgatcccgtg
    13801 ccagttcgct caggctgccg ccgtagtcgt cgagcatagc gggttgcaga ccgacgaggg
    13861 caatatgtgc tggcagatgt ccgcggatat ctgccagcgc cagcacttcg gagaaactgt
    13921 tctgatgcag gctcattttc ttcgcgctga gataagccgg aatgcgttct ccggcatagg
    13981 ttcgcagcgt tccaggttcc agcccgtagt caatggcatc gagaatcaac agatggctgg
    14041 cgctttcgac ataccccagc aagttcagtc cctgagtacc gccatcgaca atctccacat
    14101 actcgggcca gtggtaatgg gcatacagcc gttccgccac ccgcacgccg aagccttcat
    14161 cggcccacag caggttgccc agccccatga ccaccacgcg ttgctcgctc atgaacgctc
    14221 cttgttactt attttgccaa atttgtggct acggtagccg ttgaccatgg tggagatcac
    14281 cgtgtcgtcg gacatgatgt cttcacgcag cgccatgtag acatgaccga tcacaaacgc
    14341 gccaatcagc cacatcccca gccgatgcca gctgtgaatg tccattgagt tgccacccgt
    14401 ccagtagaaa aattccacca cataacggaa cggcgcaaaa atagcgtact ggctgtgttc
    14461 gctgtacagc gcaaaaccag tgatgatcat aaagaccgac atcaggaaat agccgaacat
    14521 cgccgcctgg gcgatgggat tatggcctat atcggcactc ggacgttttg ccagaaacag
    14581 ataccagcgg atttcatacc acacgccctg ccaccagctt ttacgccata ccggcacgat
    14641 aaacagctcg cgggagtatc gattgccaac aaaagcccag tagatccgca tcagcaaaac
    14701 cacggtaaaa accatcccgg cgctgaagtg aattaacctg atgtagccca tatagaacag
    14761 atacgtcgcc tcgccgctga cggaaggtag cggcttgccg ataaagtatc cggtgaccat
    14821 caacaccgcc atgcataaca ccgtcaacca gtgccagatg cggactggcg cttcaaagac
    14881 atagtggctg acaacgttgt cgcttttctg ttgcatgatg attctccttc gctgttaacg
    14941 cacctgcacg gagatcagct cgctaccgtc gtcgcccagc acgtgtgttg aacaggcgag
    15001 gcacgggtca aagctgtgca gagtacgcag gatctccagc ggttgctcgg ggatcgccat
    15061 tttggtgttc atcagcgccg cttcataagc gccaatctgc cctttgggat cgcgcgggct
    15121 ggcgttccag gtggtcggca ccacgcactg gtagagatca atcttgccat cgcgaatggc
    15181 ggcccagtgg cctaacgccc cgcgcggcgc ttcggtaaaa ccgacaccac ggcactctgt
    15241 cggccaggtt gcaggttccc atttttccgt ggaagcagtg gcgagattgc cgtttttcag
    15301 gttggtcatc agcttgtcga agaaatactg caacttacct gcggcccact gcgcttcgtg
    15361 cgcgcggcac aaaatgcggc ctaacgttga ctggataccg gaaagcggca ggttcaacgc
    15421 cgacatcatg cgatcgaccg actcaacggt cgcagcatcg cctttgtgat aagcgattaa
    15481 cgtgcgcgcc agcggcccca cttccatcgc gttaccgcgc cagcgtggcg ctttgatcca
    15541 cgagtagcgt tcctgttcat tcagctgctg aatgttggta tcgctgcctt tgacatcgcc
    15601 ggggttgtac cacgggtcgg tgatgccatc gaacggatga cgcccgacct gatcgttggg
    15661 atatcgatac caggcgtggt cgacaaactc ctgcacctgc tgcggatcaa ccaaatccac
    15721 tggcagcaca ttgttgaagt cgccgttaat caccgcgccg ccaggcatca gcagactttt
    15781 ctcgccaaag tcgttggcaa tatccgggaa tgcgccgtag ctgagaacgc atttatcaga
    15841 aagaccagtg ccgatttcgc tccacggttt gttgaactga ccgatggcta aggcgtcggg
    15901 gatcatcacg ttgttaatga agtccgccgt gcgggtgata attgactgca ccaggttcag
    15961 gcgttccata ttgactgccc cgaccgcgcc gctttcgtca atgttgatgg cgcaaggcat
    16021 cccgccgaca atccagtttg gatgcgggtt tttaccgcca aagaccgcgt ggattttcac
    16081 aatttcacgc tggaaatcga gagcttcgag gtagtgggca aagcccatca ggttggcttc
    16141 tggcggcaac ttgtactgcg ggtgccccca gtagccattg cggaagatcc ccaactgccc
    16201 gccttcaacg aattttttca ggcggttttg tacgtcgaag aaatagcccg gggatgattt
    16261 cggccaagag gagagacttt gcgccagttc ggaggttttc cgcgggtcgg ctttcagcgc
    16321 atctaacaca tcgatccagt ccatcccggc aagctgatag aagtgcacca gatgatcgtg
    16381 gcaccagagc gttgccagca taatgttgcg gatgatattg gcgttgtccg gcactttaat
    16441 accgatagca tcttcgatgg cgtaaaccga agccagggcg tgtacgccag tacagacgcc
    16501 gcagatacgt tcaacgaacg cccacgcatc gcgcgggtcg cgcccttgca gaatgatctc
    16561 cagcccgcga aacatggtgc cgcaggagac ggcattggtg atcacattct gatcgttaat
    16621 attcacttcg cagcgcatgt ggccttcgat gcgcgtaatc gggtcgacca ccaggcggcg
    16681 tccggcatta ttgatggtgt atccctgagt ttcgtactga gtgctcatgc ctgtttatcc
    16741 tcattgcctg gctgatgttc ggtttctgta ggttgctggt tatgacgtct gcgctggtca
    16801 acggcgctgg cgactgcgtg cacaccaaca gccgctgcca ccacgccaag cgcggttaaa
    16861 ccgacggtat cggcagtgga atgagtaccc atttgcggaa tatcgaccac gcggctgtag
    16921 aacgaaccgc gatcccagaa accattttcc gcacagccca ggcagccgtg accagactgg
    16981 attggaaaag aaacgccatc attccagcgt gtggaggaac aggcgttata ggtggtaggc
    17041 cctttgcagc ccattttgta caggcagtaa cctttgcggg cagcgtcatc atcccaactc
    17101 tggacgaact ctccggcgtc gaagtgggcg cggcgatagc atttatcgtg gattcgctga
    17161 ccatagaaca tcagcggacg gcccattctg tcgacatctg gcaagcgatc aaaggtcacc
    17221 atgtaagtaa tgatggcgct catcacatcc gggatcggcg ggcagccagg tactttgata
    17281 atgggtttgt cggtgatgac tttgtcgata ggcgttgcct gcgtcggatt gggccgcgcg
    17341 gcctgcacgc agccccagga cgcgcaggtt ccccaggcga taatcgcgct ggctccggca
    17401 gcggcacgtt tgagtttctc aataaacggt cgaccgctgc tgatacagaa catcccctgc
    17461 tcgcccagcg gcggattacc ttctactgcg aggatatatt tgccattgta ttgcgtgatg
    17521 atgtcttcga agacttcttc cgcctgggtt ccggcggcag ccatcaaagt atcgtcataa
    17581 tcgagggaaa tcagggaaag gatgacgtcc ttcgccagtg ggtgagcgga gcggataaaa
    17641 gattcggtac agcaggtgca ttccagaccg tggatccata ccaccggaat gcggggtttg
    17701 ttctccagcg cccaggcaat ctttggtgcc attcccgcgc ctaatcccag cgacgtggca
    17761 gccagactac aatatttgag aaagctgcgc cgggtaacgc cctgacgccg catggcctgg
    17821 taaaatgttt cctcgttatt catatcgcac gtctcttctc ctagcaacac cggcaggaca
    17881 ccgggcgctt atggtcggca agcgacgtcc ctgtagcaag gcaaaccatc gcttcacgtg
    17941 agatgctgaa aacgaaagct catccttctg cactggcaca cgtcgccaga aagtattgtt
    18001 aataaagcgt agtgaaactt ttgcacaaaa caatacaaac tgtgtggatt tatcttttag
    18061 cgataaaaat ggacctattt ttctttttgc cgggcggtgg ggatgtttag ccggttgcta
    18121 aacgagtaaa agagaaggaa ttcatggtga tgaaatgatc aatctgctgt tatttcacgc
    18181 tatcaatgca cttttggctg tttttcaggc aaacaaacaa attaggggtt tacacgccgc
    18241 atcgggatgt ttatagtgcg cgtcattccg gaagtgtggc cgagcggttg aaggcaccgg
    18301 tcttgaaaac cggcgacccg aaagggttcc agagttcgaa tctctgcgct tccgccaaac
    18361 aatcaagggg ttaccaaatg gtagcccctt tccttttttt gctcttagta tacacttggt
    18421 atactaattg gtatatttag ctcaatacac actaggtaca ttgttctctt tacgatttat
    18481 cgctataccg atgaattatg aataacccca atccatgcat gacgtgtggt gcctgttgtg
    18541 catttttccg cgtctctttt tactgggcag aagctgacga tgctggcgga aaggttccgg
    18601 tcagtctcac tgagcaaata tccccctttc atcgctgtat gcgcggcacc agtcagaaaa
    18661 atccccgatg tgtcgctctg gcgggaactc cgggtaaaaa cgcatattgc tctgtttaca
    18721 aaaaccgtcc gtccacgtgc cgggaattcg caatgtccgg agaaaatggc atcgcaaatg
    18781 aagcatgcaa ccgcgcccgg gcaaaatacg gacttccgga aatctgaaaa atcatccata
    18841 tcaacaggat gcgcgaaaaa tgagagttcc gtcacttccg gaattaactt ttccatggca
    18901 ctatttactc ggcatagtgt atatcttcac aataatgaca taatcaacgc agttcatggg
    18961 ggaggtgatg gtcccccatc tttttgtctg ttattcctgt gcccagccaa ccctgtatgc
    19021 caggatctca tccgcactgg tcagcttttc cagctctttc ttcatggtgc gctggcgaac
    19081 gtggatttcc atccctttgg tgaacatcgc ctgctctgcc gcttcactca gcgcaataag
    19141 ctcttctgct gtcaccggca catcattgtt ttccgcatcc gtccagaaaa acgcctccgg
    19201 cagtttcccc gctttcgctg cagccaccga cggctcaaga cgcgtctgcg ttgacttccc
    19261 gtagtcccat ttacgcccat tgtgctcaaa cgtgtagttc gccgcttcca tcgcattacg
    19321 ccacgcgttt atttcatcgc ccttcatccc tcgcgctttc tccgcggtca gcaggtccgt
    19381 gattttctcc ccgtcaaaac cccagcgccc gctcaggtca attttccggt tctccggggt
    19441 attcggcact tctgccacac tctggttcac cggccacagt aacgcagtgt ccttaccgta
    19501 tcccgtaatc acaccccggc tgtcatacac caccttcagc gtctccggcg aaaacaacgc
    19561 ctgacactca taccagtcct gaccatcctc agacttcagg tacatcgcgc ctgcaacatc
    19621 cggctccgcc ggggtgtaat acgaaaaatt tctgatatgc atcatgtttc cgtgccctcc
    19681 tccgataccg gcacccagac cggcattttt tccgcaccga cgccccgctt catacctgcc
    19741 ggtgcagcat ccgctgcaaa gactttatac accacataat ccaccacaat accgtcttcc
    19801 ggccagcgcc ctgctgcctc ataaaccggc cgcagggaca ggggataaaa catattctct
    19861 gacggtgaaa aaacatactg ttccatacaa tgccctcagt atccgatcgc ttcccatgaa
    19921 aacttgccgt agcatcccct gactgttatc agagtcacag acactaatga cggattcgac
    19981 agggtataca tcttaatgac gttggggtct cccactgcag acacctgagc attcagagca
    20041 gcaaccggaa aagcgacagg aaaacggata gagtgttgtg tcataagctt accatcatcc
    20101 tcacttacct caatactgcc ccattgccgc aaacgaccgg ttacttcatc acgctcccag
    20161 ccatttactg tcaggcttgc tgttgtgggc ttgttttttg tgctgtaatc cacccgccag
    20221 tgaaaacgtt gtgtgtctcc gtaaactgta caggtacaga ccgtgccgtt cagaaaacca
    20281 tcgccaccgt attcgccgat ggtgacccgg actatggctg cgttatacgt ccccatcacc
    20341 tcaatggcgc agcctcccag attgagtttt cccggtccga catccgtgat gaccttatta
    20401 aattccgcaa gcagtgatgc cttcatcatc cagtaaggct ggtcaaatgc ccccttttct
    20461 ttcagccagg ccacaaactc actcgtggtc cattcaccgg tctccgtatg aatgtcccgc
    20521 ccgtatactc tggctgctcc cacggtgttc agaaacttca ccttatccgg gatatcgtca
    20581 ccgtttttcg ctttctccag gcatccgtcc gctttgtcca ttgccgcttt caccgctttc
    20641 ggggtggctg ccttcgtttc atcatcactg tccgttgcgc tgcttaactg cacaattccc
    20701 ttctgtgccg tcgtcgcatc cggtcctccc ggctcgcctt tttcgccctt ctctccccgt
    20761 tcacctttca caccctgaag cccctgtggt cctgtctcac cacgctcacc ctttggccca
    20821 cgctcgccgg tatcgccttt tggcccggga ataccctgtg gcccggtgtc ccccttatcc
    20881 cccttcggtc cccgcgcatt ctctgcccgt ttttttgcct cctccgcact ggctgcggac
    20941 gcttccgcac gtttcaggat ttccgctgcc accgcttcca gctctgcaag ggctttcggg
    21001 taatactgtg cgtcttccag gtccatcaga tatttattca gcgttcccgg tgcagaatcc
    21061 gccttcacca gaatgtcacc cacatatgac ggcgcgtacc cttccgtgtt cagcgtcacc
    21121 cggtacaacc ccggctcaac atccatactg taactgccgg tttcccccgg ctgaccatac
    21181 gccaccgtgg tgacaatcac cgtctccgtt gtgcggcgcg ctttcagctc tatcgtgcat
    21241 cccggtaccg cttttcccgt accatccttc agcacacccg atattctgac tgtcatggat
    21301 ttcccccata aaaaaaccgc aggaccggtt tcccggcctg cggtaatatt tgtggtttgt
    21361 tggtgttaaa acggtgccat ccggctgacc accctcagca accggtcggc gggggatatt
    21421 ctcccccgcc acggtttctt actgcttaca ctgtaagaac gccgcaatct ccgcgcccgc
    21481 tatccggaac cggaactcgc acagtgaagt gtgggtgatc cagataatga gcactaccgt
    21541 gatacaaatc acggtggttt ttaacggttt ttgcgacata aacgcttgct ccttttacgg
    21601 agaggcgcta accttcaatt tgctgaagat cgaaagtcag gcctcgggtt aaacatgaat
    21661 gtttgtccgg ggccttttca catccggcct tcaggtgttc cctccggcca tcagccaaag
    21721 gcacccgcgc atactgtacg gtttttgtct c
//
LOCUS       DAZFRN010000078        21444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFRN010000078 DAZFRN010000000
VERSION     DAZFRN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.078"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..4827
                     /locus_tag="ACHKUB_002836"
     CDS             <1..4827
                     /locus_tag="ACHKUB_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6655820.1"
                     /translation="WNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGGMLSSFGAL
                     ANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIAEGQYSSAI
                     GSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALGKLSIAMGD
                     SSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEENAIALGRSS
                     VASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLAD
                     KVNTIALGDGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGD
                     KSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAI
                     NGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQW
                     DQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTANIAT
                     NTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNV
                     KDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTN
                     IATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTANKITNILDGTVTATSSD
                     AINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFST
                     SLGDALLWDATADKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVD
                     ALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSA
                     AHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAP
                     TYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSISE
                     DSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFND
                     ASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSI
                     TENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVAT
                     TPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSN
                     AQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGS
                     ADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYF
                     KTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITN
                     VAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGV
                     NNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYT
                     PGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5196..6851
                     /gene="lldP"
                     /locus_tag="ACHKUB_002837"
     CDS             5196..6851
                     /gene="lldP"
                     /locus_tag="ACHKUB_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO6655821.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6851..7627
                     /gene="lldR"
                     /locus_tag="ACHKUB_002838"
     CDS             6851..7627
                     /gene="lldR"
                     /locus_tag="ACHKUB_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO6655822.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            7624..8814
                     /gene="lldD"
                     /locus_tag="ACHKUB_002839"
     CDS             7624..8814
                     /gene="lldD"
                     /locus_tag="ACHKUB_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO6655823.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSINEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9000..9473
                     /gene="trmL"
                     /locus_tag="ACHKUB_002840"
     CDS             9000..9473
                     /gene="trmL"
                     /locus_tag="ACHKUB_002840"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO6655824.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9526..10347)
                     /gene="cysE"
                     /locus_tag="ACHKUB_002841"
     CDS             complement(9526..10347)
                     /gene="cysE"
                     /locus_tag="ACHKUB_002841"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO6655825.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10427..11446)
                     /gene="gpsA"
                     /locus_tag="ACHKUB_002842"
     CDS             complement(10427..11446)
                     /gene="gpsA"
                     /locus_tag="ACHKUB_002842"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO6655826.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11446..11913)
                     /gene="secB"
                     /locus_tag="ACHKUB_002843"
     CDS             complement(11446..11913)
                     /gene="secB"
                     /locus_tag="ACHKUB_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO6655827.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11976..12227)
                     /gene="grxC"
                     /locus_tag="ACHKUB_002844"
     CDS             complement(11976..12227)
                     /gene="grxC"
                     /locus_tag="ACHKUB_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO6655828.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12369..12800)
                     /locus_tag="ACHKUB_002845"
     CDS             complement(12369..12800)
                     /locus_tag="ACHKUB_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO6655829.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13045..14589
                     /gene="gpmM"
                     /locus_tag="ACHKUB_002846"
                     /gene_synonym="pgmI"
     CDS             13045..14589
                     /gene="gpmM"
                     /locus_tag="ACHKUB_002846"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO6655830.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14623..15882
                     /gene="envC"
                     /locus_tag="ACHKUB_002847"
     CDS             14623..15882
                     /gene="envC"
                     /locus_tag="ACHKUB_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO6655831.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15886..16845
                     /locus_tag="ACHKUB_002848"
     CDS             15886..16845
                     /locus_tag="ACHKUB_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO6655832.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(16832..17866)
                     /gene="waaH"
                     /locus_tag="ACHKUB_002849"
     CDS             complement(16832..17866)
                     /gene="waaH"
                     /locus_tag="ACHKUB_002849"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO6655833.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTIALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18105..19130)
                     /gene="tdh"
                     /locus_tag="ACHKUB_002850"
     CDS             complement(18105..19130)
                     /gene="tdh"
                     /locus_tag="ACHKUB_002850"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO6655834.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(19140..20336)
                     /gene="kbl"
                     /locus_tag="ACHKUB_002851"
     CDS             complement(19140..20336)
                     /gene="kbl"
                     /locus_tag="ACHKUB_002851"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO6655835.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGHVDIITGTLGKALGGASGGYTAARKEVIEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20611..>21444)
                     /gene="yibB"
                     /locus_tag="ACHKUB_002852"
     CDS             complement(20611..>21444)
                     /gene="yibB"
                     /locus_tag="ACHKUB_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO6655836.1"
                     /translation="TAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMII
                     FTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNP
                     EYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDE
                     SKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITL
                     NNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSR
                     K"
BASE COUNT         5380 a         5600 c         5472 g         4992 t
ORIGIN      
        1 tggaaccctg cgacagggaa ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag
       61 aaatctgggc gcagtaagct gttaatttct gcgctggttg cgggtggaat gttgtcgtcg
      121 tttggggcat tggcgaatgc cgggaatgac aacggtcagg gtgttgatta cggtagtgga
      181 tcagctggcg acggctgggt tgctataggc aaaggggcga aagcaaatac ttttatgaac
      241 accagtggtt ccagtactgc tgtgggttat gacgctatag ctgaaggcca atatagctct
      301 gccatcgggt caaaaaccca tgcgattggc ggtgcatcaa tggcctttgg ggttagtgca
      361 atatcagaag gcgatagaag tatagcactg ggtgcctctt cgtattcatt gggccaatac
      421 tcaatggccc tcggccgtta ttcaaaagca ttgggtaaat tgtctattgc tatgggggac
      481 tcttccaaag cggaaggagc aaacgccatt gccctgggaa atgccactaa agctactgag
      541 attatgagta ttgctcttgg cgacaccgcc aatgcgtcaa aagcgtattc aatggcgctg
      601 ggagcaagta gcgtcgcatc tgaagaaaac gcaattgccc tggggcgtag cagtgtagct
      661 agcggtactg acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt
      721 gcgataggtg ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag
      781 gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact
      841 atcgcattag gagatggcag ccaggctctg gcagataatg caatcgccat aggccagggc
      901 aacaaagctg atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta
      961 aacaccattg ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt
     1021 agtaatagca gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat
     1081 ttagacaata ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa
     1141 aatggcgcga tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt
     1201 agcgactcgg tagcaaaaag gcttggagga ggggctgcag tagatgttga tgacggtact
     1261 gttacagcac caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg ggctgcgctc
     1321 gctgtacttg atgaaaacac cctgcaatgg gaccaaacca aaggcaaata cagcgctgct
     1381 catggtacta gtagcccaac tgccagcgta atcaccgatg ttgcggatgg cacgatttca
     1441 gcctccagta aggatgcggt taacggttcc caactgaaag ctaccaatga cgatgtcgaa
     1501 gccaacaccg ccaatatcgc tactaatacc agcaacattg ccacgaatac ggcaaatatt
     1561 gccaccaata ccaccaatat caccaacctg acggattccg ttggtgacct tcaggctgat
     1621 gccctgctct ggaacgaaac taaaaaggca ttcagtgcag ctcacggcca ggataccacc
     1681 agcaaaatca ccaacgttaa agatgccgac ctgacggctg acagcactga tgctgttaac
     1741 ggctctcagc tgaaaaccac caacgatgct gtggcgacga ataccaccaa tatcgccaat
     1801 aacacttcca atattgccac taacaccacc aacatctcta atctgactga gacggtgact
     1861 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtattcac ggcagctcat
     1921 ggcaccgaga ccaccagcaa aatcaccaac gttaaagatg gcgacctgac gactggcagc
     1981 accgatgccg ttaacggctc tcagctgaaa accaccaacg atgccgtggc gacgaatacc
     2041 accaatatcg ccactaacac caccaacatc tctaatctga ctgagacggt gactaatctt
     2101 ggtgaggatg cgctgaaatg ggataaggac aatggtgtct tcactgcagc tcatggcaac
     2161 aataccgcca acaaaatcac caatatcctg gacggcacag tcactgcaac cagttccgat
     2221 gccattaacg gtagccagct ttatgactta agcagcaata tcgccaccta cttcggcggc
     2281 aatgcttctg tgaatactga cggtgtgttt accggtccaa cctacaaaat cggtgaaaca
     2341 aattattata acgtcggcga tgcactggct gcgattaact cctcatttag cacgtctctc
     2401 ggcgatgctc tgctttggga tgccaccgca gataaattca gtgccaaaca cggtactaat
     2461 ggtgacgcaa gcgtgatcac tgatgtcgca gatggtgaaa tttcagactc cagttctgac
     2521 gcagtaaacg gctcacaact ccacggcgtg agcagttatg ttgttgatgc gctggggggt
     2581 ggtgccgaag tcaatgcaga cggcaccatc actgcgccga cgtacaccat tgctaatgct
     2641 gattacgata atgtcggtga tgccctgaat gctatcgata ccactcttga cgacgctctg
     2701 ctctgggatg cggacgccgg tgaaaatggt gcatttagcg ccgctcacgg aaaagataaa
     2761 actgccagtg taatcactaa cgtcgctaac ggtgcaatct ctgctgccag cagcgacgcg
     2821 attaacggct cacaactcta taccaccaat aagtacatcg ctgatgcgct gggtggtgac
     2881 gcagaagtca acgctgacgg caccatcacc gcaccgactt acaccattgc gaacgccgag
     2941 tacaacaacg tcggtgacgc cctggatgcg cttgatgata acgccctgct gtgggatgag
     3001 actgccaatg gcggtgctgg agcctacaat gccagccatg acggtaaagc cagcatcatc
     3061 actaatgtcg ctaatggcag tattagtgag gacagtaccg atgcagtgaa cggttctcag
     3121 ttgaatgcga cgaatatgat gattgagcag aacacccaaa ttatcaatca gctcgctggt
     3181 aacaccgacg caacctatat ccaagaaaac ggtgcgggta ttaactatgt gcgtactaac
     3241 gacgacggct tagcgttcaa cgacgccagc gcacagggtg ttggcgctac agctataggt
     3301 tataactctg tcgccaaagg cgatagcagc gtagctattg gtcagggcag ctacagcgac
     3361 gttgatacgg gtatcgccct gggtagcagc tctgtttcca gccgagtgat tgccaaaggc
     3421 tcccgtgaca ccagcataac ggaaaatggc gttgttattg gttacgacac cacggatggc
     3481 gaactgctcg gtgcattgtc tatcggtgat gacggtaaat atcgtcaaat catcaacgta
     3541 gccgatggtt ccgaagccca tgacgccgtt acggttcgtc aattgcagaa tgcgattggt
     3601 gcggtcgcaa ccacgccgac taaatacttc cacgctaatt caacggaaga agattcactg
     3661 gcagtgggaa ctgactcgct ggcaatgggt gcgaaaacca tcgtgaatgg cgataaaggt
     3721 attggtatcg gttatggtgc ctacgtggac gcgaatgcac ttaacggcat tgccattggt
     3781 agcaatgcgc aagtcattca tgtcaacagt attgcgatag gtaatggttc tacgaccact
     3841 cgtggcgctc aaaccaatta taccgcctac aacatggacg caccgcagaa ctctgtcggt
     3901 gaattctcag tcggtagtgc ggatggtcaa cgtcagatca ctaacgtcgc agcaggttcg
     3961 gctgataccg atgcggtcaa cgtgggtcag ttgaaagtaa cggatgcgca ggtttcccag
     4021 aatacccaga gcattactaa cctggataat cgggtaacga atcttgattc acgcgtcacc
     4081 aatatcgaaa acggtattgg cgatatcgtc accaccggta gcaccaagta cttcaagacc
     4141 aataccgatg gtgtagatgc cagcgcgcag ggtaaagata gcgtcgcgat tggttctggc
     4201 tccattgctg ccgcggataa cagcgtcgca ctgggtacag ggtctgtggc aaccgaagaa
     4261 aatacgatct ctgtaggctc atctactaat caacgtcgta tcaccaacgt agccgcaggt
     4321 aaaaacgaca ccgatgctgt taacgtggca cagttgaagt cttccgaagc tggcggtgtg
     4381 cgttacgaca ccaaagctga tggttctatc gactatagca atatcaccct cggtggcggc
     4441 aacggcggta cgactcgtat cagcaacgtc tccgctggcg tcaacaacaa cgacgcggtg
     4501 aattacgcgc agttgaagca aagcgtgcag gaaacgaagc aatacaccga tcagcgaatg
     4561 gttgagatgg ataacaaact gtctaaaact gaaagcaagt tgagcggtgg tatcgcttct
     4621 gcaatggcaa tgaccggtct gccgcaggct tatacaccgg gtgccagcat ggcttctatt
     4681 ggtggcggta cttacaacgg tgaatcggca gttgctttag gtgtatcgat ggtgagcgcc
     4741 aatggtcgtt gggtctacaa attacaaggt agtaccaata gccagggtga atactccgcc
     4801 gcactcggtg ccggtattca gtggtaatga tccattaagt tagtgtgact aagggcaaac
     4861 gccaaattgc ctgatgcgtt acgcgcatca ggcaagataa ggcgctccgg gtcaacaatc
     4921 taagggcaat tctctgatga ggattgccct ttttctttac cagacatctc cccccacaag
     4981 aattggccct accaattctt cgcttatctg acctctggtt cacaattccc caattaaaac
     5041 tcacatcaat attgccaata cataacattt agttaaccat tcattgtcat tatccctaca
     5101 caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag caacagactc
     5161 attacacgat gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa caaaactacg
     5221 atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc ctgtttttct
     5281 tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg acggtagcaa
     5341 tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg ctggcctcgg
     5401 tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg gatcattatt gcagcagtgt
     5461 tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg tctattcttt
     5521 cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc ggcgctttcc
     5581 ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg ctggtcggcc
     5641 tgggttttaa accgctgtac gccgccggac tgtgcctgat tgttaacacc gcgccagtgg
     5701 catttggcgc gatgggcatt ccaatcctgg ttgccggaca agtaacaggc atcgacagct
     5761 ttgagattgg ccagatggtg gggcggcagc taccgtttat gaccattatc gtgctgttct
     5821 ggatcatggc gattatggac ggctggcgcg gtatcaagga gacgtggcct gcggtcgtgg
     5881 ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt gggccggagc
     5941 tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc ctcaaacgct
     6001 ggcagccagt gcgcgtattc cgttttggtg atttgggggc gtcacaggtt gatatgacgc
     6061 tggcccacac cggttacact gcgggtcagg tgttacgtgc ctggacaccg ttcctgttcc
     6121 tgacagctac cgtaacactg tggagtatcc cgccgtttaa agccctgttc gcatcgggtg
     6181 gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa ctggttgccc
     6241 gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag tttgactggt
     6301 tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc tggctgaaga
     6361 tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg gctctaccca
     6421 tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc ggactgtcat
     6481 caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc tcgccgttcc
     6541 tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc ctgttcgccg
     6601 cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg gttgccgcca
     6661 ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct atcgcctgtg
     6721 cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc aaacacagcc
     6781 tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc ttaacgtgga
     6841 tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg atcgtgtgcg
     6901 ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg agcgccaact
     6961 ggcgatgcaa ctcggcgtat cacgtaattc actacgcgag gcgctggcaa aactggtgag
     7021 cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc gtcatgacac
     7081 atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg atccggatta
     7141 cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat ggcatgcggc
     7201 aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag caacgctaag
     7261 tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga ttgccgaagc
     7321 ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc tgcaatcctc
     7381 agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac aactgaccga
     7441 acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg cgcgtaaagc
     7501 aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg aagatcaggc
     7561 tcgccacgca cggattaccc gcctgcccgg tgagcattat gagcattcga gggagaaaaa
     7621 cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat tctgccgccg
     7681 ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg ccgcaacgtg
     7741 gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc tgacttaagc
     7801 ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc accggtgggt
     7861 ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc ggacgcgcat
     7921 ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt cgcgccagcc
     7981 atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt tatgcgtaac
     8041 gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt ggatatgccg
     8101 acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa tgcggcaatg
     8161 cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg cctgaacggt
     8221 cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg actggaagat
     8281 tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga ccttgagtgg
     8341 atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc ggaagatgcg
     8401 cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg tggccgccag
     8461 ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc ggtgaaaggt
     8521 gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt gcgtatgatt
     8581 gcgctcggtg ccgacaccgt acttctgggt cgtgctttcc tgtatgcgct ggcaacagcg
     8641 ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaga aagagatgaa agtggcaatg
     8701 acgctgactg gcgcgaaatc gatcaatgaa attacgcaag attcgctggt gcaggggctg
     8761 ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc atagtcgttt
     8821 gagccccctc accctaaccc tctccctcag ggagtgggga ccgttcggcg ctgtatgtac
     8881 tccctcactc tgaaacgaca ccgcactctt tttttctccc tcgcccctcc ggggtgaggg
     8941 gaaaaggccg cactgctcac caattttctg ctatcctgcc cccgcactaa gggggcagta
     9001 tgctaaacat cgtactttac gaaccagaaa ttccgccaaa tactggcaat atcatccgtc
     9061 tttgcgctaa taccggcttt cgtctgcata tcatcgaacc gatgggattt gcctgggacg
     9121 ataagcgcct gcgccgcgcg gggctggact atcacgagtt taccgccgtt acacgtcatc
     9181 atgactttcg cgcgttcctc gaagcagaaa atccccagcg cctgtttgcc ctcaccacga
     9241 aaggtacgcc tgctcacagc gccgtaaact atcaggatgg cgattatctg atgttcggcc
     9301 cggaaacacg cggcctgcca gcgagcattc ttgatgccct gcccgctgaa caaaaaattc
     9361 gcattccgat ggtgccggac agccgcagca tgaacctgtc caatgcggtg tcagttgtgg
     9421 tgtatgaagc ctggaggcag ctgggatatc cgggagcgtt gttgagagat tagttactgt
     9481 atgccggata gatgattaca tcgcatccgg cacgatcaca ggacatcaga tcccatcccc
     9541 atactcaaat gtatggttaa taccgttgaa atgctggtcc atatccattg atggcttatc
     9601 gctgtctggt ttaccgacaa tacgagccgg aacgccagcg gcggttgtat gcggcggcac
     9661 cggttgcagc accacggaac ctgcgccaat cttcgcgccg cgaccaactt caatattgcc
     9721 gaggattttc gcgcccgcgc caatcatcac accttcacga attttcgggt gacggtcacc
     9781 accagattta cccgtaccgc caagcgtcac agattgcaga atcgatacgt cgttttcaat
     9841 taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg agcataatgc cacggccaat
     9901 ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa acctggtttt gcagaaagat
     9961 tgccaatgcg cgacgcccct gattccacaa ccagtgaccg atgcgatagg cctgcaaggc
    10021 atgaaaaccc ttcaggtata acaacggggt tgagtattta tcgactgccg ggtcgcgggt
    10081 acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt tccgggtcag cggcgtaggc
    10141 ttcttccacc acttcacgga tagcaatagc aggcataatt ggcgatgaca gcttgttcgc
    10201 cagcatgtag ctcagtgcac taccaaggtt ttcgtgcttg agtagcgtcg cgtggtaaaa
    10261 actggccagc attggctcac agtccgccag cgttctggct tcggctttaa tattgttcca
    10321 gacaatttcc agttcttcac acgacattgc ttactccaca cgatgagata atgaccggcc
    10381 cgttctgcgc gggccgggtc atagcggtaa caaaggttcc ctggggttag tggctgctgc
    10441 gctcgtcctt gcgtgcacga cctaataacg tcaatgctgc ctcgcgcgcg ttttttccgc
    10501 aatataatac ttgataaatt tcctcggtta ttggcatttc aacgccgaag cgatgcgcca
    10561 gttcgcggac ttctttcgta ttgcggtagc cttccaccac ctgaccaatc ttctcctgcg
    10621 cgctttgtac atccatgccc tgaccgagca tcatgccaaa acggcggtta cgcgactggt
    10681 tgtcggtaca ggtaagcacc agatcgccca gccccgccat gcccataaag gtggcaggat
    10741 cggcacccag cgccgcacca agacgcgaca tttcagccag cccacgggtg atcagcgccg
    10801 tacgcgcatt cgcaccaaaa ccgataccgt cggacatccc cgcaccaatg gcaatgacgt
    10861 ttttcaccgc gccgccaagc tgcacgccaa tgaaatccgg attgctgtaa acgcggaaac
    10921 ttttgccaca gtgcagcagc tgctggagat catcggcaaa ggtctgatcg gtcgaggcca
    10981 gcgaaatagc tgtcggtaaa cctgccgcca gttctttcgc aaacgttggg ccagagataa
    11041 ccgccagcgg aatttgatcg cctaacgcct cgcgcgccac gtcctgtaac agacgtccgg
    11101 tttccgcttc cagccctttg gtcgcccaca ccagacgcgc atcaggacgc atcagcggtt
    11161 taatctggcg cagcacttca ccaaagacat ggctgggtac gacgacaaga atattacggc
    11221 tggcggccag tgcggtggcg agatcgcttt caagatggag cgtatcgggg aaaggcacat
    11281 cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt tgcgatatgt tcagggtcat
    11341 ggccccagag gacaacctcg tggccatttc ttgccagggt aatggcaaga gcggtgccgt
    11401 acgagccggc accgatcaca gtcattgaag cattacgttg gttcatcagg catcctgatg
    11461 ttcttcagta ccttcgccag cctgctgctg caaatagttc atgaacagcg catcgaagtt
    11521 aaccggcgca aggttcagtt gcgggaatgt accgcgggat accatgctgg tgatgcactc
    11581 acgagcatac gggaacagaa tgttcgggca gtatgcgccc aggcaatgtg ccatctgggt
    11641 gccttcgata cccgcgatgg agaaaatacc gccctgctga acttcacaca ggaacgcggt
    11701 ttcttcgccc agggaggccg ttacggtaac acgcagtacc acttcgtata cgtcatctgc
    11761 cagttgggaa gatgccgtat ccagatcaag tttaacttct ggttgccaat ctttctggaa
    11821 aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg gtataaatac gttggatctg
    11881 gaaagtcatt tcagtgttgt tttgttctga catgtgtaga aaacccttaa gtgttgtcct
    11941 taaatactgc gcaatgtaat gccaacgttc acacgttatt tcagcagggg atccagtcca
    12001 ccacgtgcat ccaatgcata caagtcatca cagccgccaa tgtgctgtgc gtcaataaaa
    12061 atctggggaa ccgtggtgcg accgctgcgt ttgatcatct cttcacgctt ggcggcattg
    12121 ccatcgatcg gcaactcctg aaaactcacg cccttgctgc tcagcagtgc ttttgcacga
    12181 tggcaatacg ggcaggtttc tttggtatag atttcaacat tggccataat ttagctcctg
    12241 tttttttacc ctgaaatttc atgttgcagg gcggctgcaa aatggagtgt ccccagaagc
    12301 ttataacaat aagtcgctgg gggcctaatt gcagctaacg gcctgcatca tgaaagacga
    12361 caggtaaatt atttgccgcg caccagaggc aagttttcgc cagcccagcc agcaacgccc
    12421 tctttcagca cgaatacttg cgcaaaacca gctttcgtca gcgcgtttgc aggctcctgg
    12481 cactgcatgc cagaaccgtc taccacgata accggtttgt ctttgtgctt ctcaagctca
    12541 ccaacattgt tggctttgat ttcactcggc aacagattga tagaacctgc gatatggcct
    12601 ttacggaagt catcacgctg acgtaaatcc acaaccacag cgtcttcttt gttgatcaga
    12661 cgcgtagctt caccacgagt aatcaccttc actttcgagg tcaggctctt aaacgtagtc
    12721 acaagaaccg ccaccagtaa cgcgatccag gcgatactca gtatgggatg acggccaaca
    12781 aattgcataa tttcttgcat ggggggtaac aactcccgac gtagtgatta aaaaaaccag
    12841 gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc gcgttaactg gaatgcaatt
    12901 ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg gcttgccgcg agcggtcgta
    12961 tattttgatc ttcagaggct attttatcga ttcagctgta gtaaaattac gcaaattttg
    13021 actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa aacctatggt actggtgatt
    13081 ctggatggct atggctatcg tgaagaacag caggataacg ccatttttag tgctaaaacc
    13141 ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc tgatcgacgc ttccggtctg
    13201 gaagtcggtc tgcctgaccg tcagatgggt aactccgaag taggccacgt taacctgggt
    13261 gccggccgca tcgtgtatca ggacctgact cgtctggacg ttgaaatcaa agatcgcgct
    13321 ttctttgcta atccagtgct gactggcgcg gtagataaag cgaaaaacgc aggtaaagca
    13381 gtacacatta tgggtctgct ctccgcaggc ggcgtacaca gccacgaaga tcacatcatg
    13441 gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa tctacctgca cgcattcctt
    13501 gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc tgaaaaaatt tgaagagaaa
    13561 tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg gtcgttacta cgcgatggac
    13621 cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc tgctgactct ggcgcagggt
    13681 gagttccagg ccgatactgc cgttgctggt ttgcaggcag cttatgctcg cgacgaaaac
    13741 gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc agccagatgc ggcaatggaa
    13801 gacggcgatg cgctgatttt catgaacttc cgtgctgacc gcgcgcgtga aatcactcgt
    13861 gcattcgtga acgctgattt cgatggcttc gcgcgtaaga aagtggttaa cgtcgatttc
    13921 gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg ttgcttaccc acccgcttcc
    13981 ctggttaaca ccttcggcga gtggatggcg aaaaacgaca aaactcagtt gcgtatttcc
    14041 gaaaccgaaa aatatgccca cgttactttc ttcttcaacg gtggcgtaga agagtcgttc
    14101 aaaggcgaag atcgcattct gatcaactca ccgaaagtgg ctacctacga tctgcaaccg
    14161 gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg ccatcaagag cggcaaatac
    14221 gacaccatta tctgtaacta tccgaacggc gacatggtag gtcacaccgg ggtgatggaa
    14281 gcggcggtta aagcggttga agcactggat cactgcgtgg aagaagtcgc gaaagcggtt
    14341 gagtccgtgg gtggacaact gctgatcacc gctgaccacg gtaacgctga acagatgcgc
    14401 gatccggcaa cgggtcaggc acacacggca cacaccaacc tgccagttcc gctgatttat
    14461 gttggtgata agaacgtgaa agcggttgaa ggcggcaaac tttctgacat cgcgccgacc
    14521 atgttgtcgc tgatgggtat ggaaatcccg caagagatga ctggtaagcc gctgttcatc
    14581 gtggaataat ccctccccat gaggggaaag gcgattaata ccatgacacg ggccgtgaaa
    14641 ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg ttcttagcgc tggcgtattg
    14701 ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac tcaaatctat tcaggccgat
    14761 atcgccgcaa aagagcgcgc ggtacgccaa aagcaacaac aacgcgcaag cctgctcgca
    14821 caattgaaaa agcaggaaga agcgatctct gaagccaccc gtaagctgcg cgaaacgcaa
    14881 aacacgctca atcaactcaa taaacagatt gatgagatga acgcgtcgat tgccaaactg
    14941 gagcagcaaa aagccgccca ggagcgcagc ctcgccgcgc aactggatgc cgcattccgt
    15001 cagggcgagc ataccggtat tcagctaatt ctcagcggtg aagaaagcca gcgtggacag
    15061 cgtttacagg cttatttcgg ctatctcaac caggcgcgac aagaaaccat tgcccagttg
    15121 aagcaaacgc gtgaagaagt cgccatgcag cgtgctgaac tggaagagaa acaaagcgag
    15181 caacaaacgc tgttatatga acagcgcgcc caacaggcga aactgactca ggctctgaac
    15241 gagcgtaaaa agacgctggc ggggctggaa tcttccatcc agcaaggtca gcagcagcta
    15301 agtgaactgc gtgccaacga atcccgtctg cgtaacagca ttgcccgtgc ggaagccgcg
    15361 gcgaaagcgc gtgctgaacg agaagcgcgt gaggcccagg cggttcgcga ccgccagaaa
    15421 gaagcgacgc gcaaaggcac cacctataaa ccgaccgaaa gcgaaaaatc gctgatgtcc
    15481 cgaactggtg gcctgggtgc gccgcgtggt caggcattct ggccggttcg cgggccgacg
    15541 ctgcatcgct atggtgaaca gctacagggc gaactacgct ggaaaggaat ggttatcggt
    15601 gcctctgaag gtactgaagt taaagcgatt gccgacggtc gggtgattct ggctgactgg
    15661 ctgcaaggtt acggtctggt ggtggtggtt gagcacggta aaggcgacat gagtctttac
    15721 ggctataatc agagcgcact ggtgagcgtt ggttcgcagg ttcgcgcggg ccagccaatt
    15781 gcactggtgg gcagcagtgg cggtcagggt cggccttcac tctatttcga aattcgccgc
    15841 cagggtcagg cggtcaatcc acagccgtgg ttgggaagat aagttttgtt tccatttcgt
    15901 cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct cccccgtact tgctggcaaa
    15961 cttgccatcg tcattgatga ttttgggtat cgcccgcaca acgaaaacca ggtgctggcg
    16021 atgccctccg ctatctccgt cgctgtatta cccgattcac cgcacgccag agagatggcg
    16081 accaaagcgc ataacagcgg gcacgaagtg ttgattcatc tcccgatggc accactgagt
    16141 aaacagccgc tggagaaaaa tacgctgcgc ccggagatga gcagcgacga aattgagcgc
    16201 attattcgta gtgcggtcaa taacgtgccc tatgccgtgg ggatcaacaa ccacatgggt
    16261 agcaagatga cctctaacct gtttggtatg cagaaagtga tgcaggcgct ggagcgttac
    16321 aatctttact tcctcgacag cgtaaccatc ggtaataccc aggcgatgcg cgccgcgcaa
    16381 ggcactggcg tgaaggtgat caaacggaag gtattcctcg acgattcgca aaatgaagcg
    16441 gacatccgtg tgcaatttaa tcgcgcaatt gatctggcgc gtcgcaacgg ttcaaccatt
    16501 gccattgggc atcctcaccc ttcaacggta cgcgtgctac aacagatggt ttataacctg
    16561 ccgccagaca ttacgctggt gaaagccagc agcttgctga atgaaccgca ggttgatacg
    16621 tctacaccgc cgaaaaacac tgtgcccgac acaccgcgta atccgttccg tggcgtgaag
    16681 ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta atcgcttctt tgaagtatta
    16741 agcgaaagca tcagccagag cacgctgatc gtttacttcc agcatcagtg gcaaggctgg
    16801 ggcaaacagc ccgaagcggc aaaacttaac gctagcgcaa attaaaggcg ctacgcgtaa
    16861 tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa gcggaaagac cacagtaacg
    16921 cctggtaacc caccttcacg ctgcgcacat tggtaatcag gcgcttatac ataccggaag
    16981 taaatatctc ggcaatcatc cgttggcggg taagaatatc cggctctttg cgcaccgcat
    17041 ggcaaacacg caatgcttcg taagttattt gctgatgaaa ttccggataa atcgtaattt
    17101 tgtcggcata atttcgattt aatttctcca gcaggcgggt aatcttaata tagtgacgtt
    17161 gataattaag gtttttattc ccttgtctat gcaaccgact cactgacgta ttatgcagat
    17221 aatatttata taatgattgc tcggtatatc gcgcacgcag cgcattaaac atgaattctg
    17281 ttgtccagac aatatcctga tgatgtaatc cggcaataaa tttaatgtta tttttaacaa
    17341 taacatcgcg gcgataaacc cccatccaga caacgtgagt ccaacggcgc gaagaaagcc
    17401 ccatccgcag ccagtccggg ccggttaata caccggttga gcgaaggcga tcgctgggga
    17461 tggattgcca ggtttctccc gtttcacgaa aagaccagtc agcgttgcac tgcgccacgt
    17521 cgaggtcgtc ctctaacgct atggtcatca gcgtttcgta catggtggga tagacttcat
    17581 cgtcagcatc gacaaaagcg acatatttgc ccgttgccac ttcaatccca cgattacgcg
    17641 ccaccgatgc gccagcattc gcctgatgca acaaacgaac gtgcggatag ttttctgcgt
    17701 aatgctttgc tatttcaaca gaattatccg ttgaaccatc gttaataata atgatttcca
    17761 gagcagtcca ggtttgcgta attaaagatt ccatacaagt gcggaaatca tcgcccgcat
    17821 tatataacgg aataataaca ctaagtttat tggtgctgtt catcataaat taccgacgac
    17881 ctctgaagtt atcctgacgt tttacaggct ttgtattaag aaactattaa gcctgtgaga
    17941 tattattttt acaggtgaga gagtcacttc agggtagaga gataatgaga gcagctatat
    18001 aagagatcag cgcaattacg atatctttat tacactgaca ggattttaca tatttaacgg
    18061 attatcctca ggcaaaaggg ctggaatacc agcccttgtt cgtgttaatc ccaactcaga
    18121 attactttcc cggactggcc cgaacgcata gcgtcaaagc ccttctggaa atcatcgata
    18181 gagaaacgat gggtaatgat cggcgagaga tccagacctg actgaatcag cgccgccatc
    18241 ttgtaccagg tttcaaacat ctcacgaccg taaatacctt taatgaacaa gcctttaaag
    18301 attactttgg tccagtcgat agacatatcg gacggtggaa tacccagcat cgcaatgcga
    18361 ccgccgtggt tcatggtgtc gagcatggta cgaaacgctg gcggcgcacc ggacatttcc
    18421 agaccgacat caaagccttc ggtcatgccc agttctgcca tcacatcatt aaggttttct
    18481 ttagcgacgt tgaccgcacg ggtgataccc attttgcgcg ccagttcgag acgatattca
    18541 ttaacatcag tgatcaccac attgcgtgca ccaacgtgtt tcgccaccgc cgcagccata
    18601 atacctatcg gacctgcacc agagaccagc acatcctcgc caaccagatc gaacgacagc
    18661 gcggtatgca cggcgttacc gaagggatca aaaatggaag ccaggtcgtc ggagatattg
    18721 tctgggattt tgaaggcgtt gaacgccggg atcaccagat attcggcgaa gcagcccgga
    18781 cggttaacgc cgacgccgat cgtgttgcgg cacagatggg tacgaccgcc acgacagttg
    18841 cgacagtgac cgcaagtgat atggccttcg ccagaaacgc gatcgccaat cttgaagcct
    18901 ttcacttcct gaccaatacc taccacttca ccgacatatt catggcccac gaccatcggc
    18961 accgggatgg ttttttgcga ccactcatcc cagttataga tgtgaacgtc agtcccgcag
    19021 atggctgttt tacggatttt aatcagcaga tcgttatgac ccagttccgg tacaggaacg
    19081 tcggtcatcc agatgccctc ttccgctttc agtttggata acgctttcat ctcacatcct
    19141 caggcaataa cgcccagttg tttaccaata cgcgtgaatg cttctactgc acgcgtaatt
    19201 tgctcagggg tatgcgccgc agacatctgg gtacgaatac gcgcctgacc tttcggaacg
    19261 accggataga agaaaccggt gacgtaaatg ccctcttttt gcagctcacg ggcaaatttc
    19321 tgcgccacta ccgcatcgcc aagcatgacc ggaataatgg catgatcggc tcccgccagg
    19381 gtaaagcccg ccgccgacat ttgctcacgg aactgacgcg cgttcgccca cagacggtca
    19441 cgcagctcgc tacccgcttc aaccatctcc agcactttga tagaagcggc aacaatggcc
    19501 ggagccagcg agttggagaa caggtacgga cgagaacgct ggcgcagcca ctcaatcact
    19561 tctttgcgcg ccgcggtata accaccagaa gccccgccca gcgctttacc gagcgtgccg
    19621 gtgatgatgt cgacatgccc catcacatcg cagtattcat gggaaccacg accattttca
    19681 ccgacaaaac cgaccgcgtg ggagtcgtct accatcacca gggcatcgta tttatctgcc
    19741 agatcgcaaa cgcccttcag gttggcaatc acgccgtcca tcgagaacac accatcggtg
    19801 gcaatcagca catgacgcgc accggcttca cgcgcttctt tcagacgagc ttcaagctcc
    19861 tgcatatcgt tgttggcata gcgatagcgt ttagctttac acagacgcac accatcaata
    19921 atagacgcgt ggttcagtgc gtcggagata atggcgtctt ccgcacccag cagcgtttca
    19981 aacaggccac cgttagcatc aaagcaggaa gagtagagaa tcgcatcttc catccccagg
    20041 aaagccgcca gtttttgttc aagctcttta tggctgtcct gagtgccgca aataaaacgc
    20101 accgaagcca tgccgaaacc gtgagaatcc attcccgcct ttgccgccgc aatcagatca
    20161 ggatgattcg ccagcccgag atagttgttg gcacaaaagt taatgacgtg gcttccatca
    20221 gccacagtga tatctgcttg ctgcgcagac gtaataatgc gctcttcttt aaacaaccct
    20281 tccgcccgtg cggtttccag atcgttggtt aactgctgat aaaattctcc acgcattgcg
    20341 attctccaga cagggcaaat tccagcacat attacccaaa cttataggtc gcgacgagat
    20401 gacgcgttaa cacttctgca aaattcagga taaataacgt gtaacttgat gtctctcaac
    20461 acctacagat agtagaaaaa ctgtacagtt ttggctgggc ataatgatga ttgattttac
    20521 tgcattgata taaaaagaaa aaatgatgcc aaagcatcat ttttcctatc tcagttggta
    20581 tcaaaacgct acccactaat ggagggaaac ctattttctt gaaaggaaaa ttctcagcgc
    20641 ctgcattttt gcccctaaag tgttactcct gaagcagcga aacaaatcga accattttcc
    20701 tctccccaga tagtttaggt tgaagagatc ggggcgtttg taatagcaca tgacaaaaat
    20761 gccctggtca tcatccacaa tgttattatt tagtgttatt ttttgacttt ccagaactaa
    20821 tttatagaat tctttccatt tatgctgtga accaacaatt gcgcccccaa taatatatac
    20881 atgattgcca atcatgaaat caaaaacctg ttgttgagat gtgacagtaa gccctttttt
    20941 tatagtgaaa agatgcatct tactttcatc aaatgggaaa tcccaaattt tcaatcctcg
    21001 agttacattt ggcttacggc aatagccaaa atctatccac gcaacaagag gcgttttcac
    21061 taagcccatg ttgatagctt tatttacaaa ataggcctta aggttacata ccaaaacata
    21121 ctctggtgac cagtactctg ggttttttaa ttgtcgaggc tcaagtctat ttgtaaacga
    21181 ctcatctttt tgaatttttt cgatacgact tctgatatat ctaaatttct tttttatatc
    21241 gataacgata accgttgttg gttttccgtt gcgaatcgct tcaacccttg gtttcaaatc
    21301 gggagaagtg aaaataatca tctcattttc aagtgcggcc agacgctcaa agtaactaaa
    21361 ataaacatca actgaacgag ctagtttttc acggaaccct ttattagcag tccaatcccc
    21421 tctaccaatg tcaaaatatg cagt
//
LOCUS       DAZFRN010000079        21441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFRN010000079 DAZFRN010000000
VERSION     DAZFRN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.079"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..205
                     /locus_tag="ACHKUB_002853"
     CDS             <1..205
                     /locus_tag="ACHKUB_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655837.1"
                     /translation="FRKEHQVKVIIQTTAVPVEAQVAADLAAVAVLLEVAAHPAAGNS
                     TIKNASPEAGIFKSDKVSLMPGL"
     gene            complement(189..1487)
                     /gene="eno"
                     /locus_tag="ACHKUB_002854"
     CDS             complement(189..1487)
                     /gene="eno"
                     /locus_tag="ACHKUB_002854"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO6655838.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1575..3212)
                     /gene="pyrG"
                     /locus_tag="ACHKUB_002855"
     CDS             complement(1575..3212)
                     /gene="pyrG"
                     /locus_tag="ACHKUB_002855"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO6655839.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3440..4231)
                     /gene="mazG"
                     /locus_tag="ACHKUB_002856"
     CDS             complement(3440..4231)
                     /gene="mazG"
                     /locus_tag="ACHKUB_002856"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO6655840.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4302..4637)
                     /gene="mazF"
                     /locus_tag="ACHKUB_002857"
     CDS             complement(4302..4637)
                     /gene="mazF"
                     /locus_tag="ACHKUB_002857"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO6655841.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4637..4885)
                     /gene="mazE"
                     /locus_tag="ACHKUB_002858"
     CDS             complement(4637..4885)
                     /gene="mazE"
                     /locus_tag="ACHKUB_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO6655842.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4963..7197)
                     /gene="relA"
                     /locus_tag="ACHKUB_002859"
     CDS             complement(4963..7197)
                     /gene="relA"
                     /locus_tag="ACHKUB_002859"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO6655843.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7245..8546)
                     /gene="rlmD"
                     /locus_tag="ACHKUB_002860"
     CDS             complement(7245..8546)
                     /gene="rlmD"
                     /locus_tag="ACHKUB_002860"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO6655844.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPKLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8603..11359
                     /gene="barA"
                     /locus_tag="ACHKUB_002861"
     CDS             8603..11359
                     /gene="barA"
                     /locus_tag="ACHKUB_002861"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO6655845.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11591..12931)
                     /gene="gudD"
                     /locus_tag="ACHKUB_002862"
     CDS             complement(11591..12931)
                     /gene="gudD"
                     /locus_tag="ACHKUB_002862"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO6655846.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12952..14292)
                     /locus_tag="ACHKUB_002863"
     CDS             complement(12952..14292)
                     /locus_tag="ACHKUB_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO6655847.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHSAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHIGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14294..15646)
                     /gene="gudP"
                     /locus_tag="ACHKUB_002864"
     CDS             complement(14294..15646)
                     /gene="gudP"
                     /locus_tag="ACHKUB_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO6655848.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16081..16530)
                     /locus_tag="ACHKUB_002865"
     CDS             complement(16081..16530)
                     /locus_tag="ACHKUB_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO6655849.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNLCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16548..17330)
                     /gene="truC"
                     /locus_tag="ACHKUB_002866"
     CDS             complement(16548..17330)
                     /gene="truC"
                     /locus_tag="ACHKUB_002866"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO6655850.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17330..17659)
                     /locus_tag="ACHKUB_002867"
     CDS             complement(17330..17659)
                     /locus_tag="ACHKUB_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO6655851.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18281..18826)
                     /gene="syd"
                     /locus_tag="ACHKUB_002868"
     CDS             complement(18281..18826)
                     /gene="syd"
                     /locus_tag="ACHKUB_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO6655852.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18894..19742
                     /gene="queF"
                     /locus_tag="ACHKUB_002869"
     CDS             18894..19742
                     /gene="queF"
                     /locus_tag="ACHKUB_002869"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO6655853.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19854..21218
                     /gene="ppnN"
                     /locus_tag="ACHKUB_002870"
     CDS             19854..21218
                     /gene="ppnN"
                     /locus_tag="ACHKUB_002870"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO6655854.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5239 a         5761 c         5125 g         5316 t
ORIGIN      
        1 cttcagaaaa gagcatcagg taaaagttat cattcagaca acagcggttc cggtggaggc
       61 tcaagtggcg gcggatttag cggcggtggc ggttcttctg gaggtggcgg cgcatccggc
      121 cgctggtaac tcaaccataa aaaatgccag cccggaggct ggcattttta aatcagataa
      181 agtcagtctt atgcctggcc tttgatctct ttacgaccgt tgtacggtgc tttttcgccc
      241 agagcttctt cgatacgaat cagctggttg tatttagcaa cacggtcaga acggctcata
      301 gaaccagttt tgatctggcc tgcagcagta ccaacagcca ggtcagcgat ggtagcgtct
      361 tcagtttcgc cagaacggtg agagataact gcagtgtagc cagcatcttt cgccatcttg
      421 attgcagcca gagtttcggt cagagaaccg atctggttga atttgatcag gatggagtta
      481 gcgatacctt tttcgatacc ttctttcagg atcttggtgt tggttacgaa caggtcgtca
      541 ccaaccagct ggattttgtc gcccagaact ttggtctggt atgcgaaacc gtcccagtca
      601 gattcgtcca gaccgtcttc gatagaaacg atcgggtact gtttggtcag ttcttccagg
      661 aagtgagtga attcttcaga ggtgaacgct ttgttgcctt cgccagccag aacgtattta
      721 ccatctttgt agaattcaga agctgcgcag tccatcgcca gagtgatgtc tttgcccagt
      781 tcataaccag cagctttaac agcttcagcg ataacagcca gagcttcagc gttggaaccc
      841 aggttcggcg catagccacc ttcgtcacca acagcagtgt tcatgccttt cgctttcaga
      901 acttttgcca ggtgatggaa aacttcagaa cccatgcgga tggcttcttt cacagttttc
      961 gcgccaaccg gctgaatcat gaattcctgg atatcaacgt tgttgtcagc gtgctcacca
     1021 ccgttgatga tgttcatcat cggaaccggc atagagtatt tgcccggagt accgttcagt
     1081 tcagcgatgt gctcgtacag cggcatacct ttagcagctg cagcagcttt ggcgttagcc
     1141 agagatacag ccaggattgc gttcgcgccg aatttggatt tgttttcggt gccgtccagg
     1201 tcgatcatga tcttgtcaat gccagcctga tctttagcat ctttgccaat cagcgcctga
     1261 gcgatcgggc cgtttaccgc agcaacagct ttggttacgc ctttacccag gaaacgggat
     1321 ttgtcgccat cgcgcagttc cagagcttca cgggaaccag tagaagcacc tgacggagca
     1381 gctgccatac cgacgaaacc accctccaga tgtacttcgg cttcaacagt cgggttacca
     1441 cgggagtcga tgatttcacg accgatgatt tttacgattt tggacattag gttttcctca
     1501 agtcactagt taaactgaaa ctccagacaa acaacgcgta cccagggtac gcgttgccgt
     1561 tctaactttt ttacttactt cgcctgacgt ttctggaact cgctggcggc tttcacaaag
     1621 cctgcaaaca gcgggtgacc atcacgtgga gtagaagtaa actccggatg gaactggcaa
     1681 gccacgaacc acgggtgatt cggaacttcg atgatctcga ccaactgatc atccccggaa
     1741 cggcccgcaa cgcgcagacc tgcatcttca atctgtttca acagcatgtt gttgacttcg
     1801 taacggtgac gatgacgctc aacaattgtc ggcgcattgt acagctggcg aaccaggcta
     1861 tcgtcaacca actggcactg ctgtgcgccg agacgcatgg taccgccgag atcgctcttc
     1921 tcgctacgaa cttcaacgtt gccgttttca tcgcgccact cggtaatcag cgccacaacc
     1981 gggtacttac agtctggcac aaattccgta gagttggcgt tctccatgtt ggcaacatgg
     2041 cgagcgtaat caattaacgc cacctgcata cccaggcaaa tgcccagata aggaatattg
     2101 ttctcacgcg caaaacgcgc ggtcgtaatc atgccttcta cgccacgata gccgaaaccg
     2161 ccaggtacga ggattgcgtc cagaccttta aggatttcaa cgccgcgcgt ttcaacatct
     2221 tgtgaatcga tcagtttgat gttgacgctg acacgattct tcagcccacc gtgtttcagt
     2281 gcttcgatca ctgatttata agcatccggc agttcaatgt acttgccgac cataccgatg
     2341 gtgacttcac ttaccgggtt cgcttcttcg aagataacct gttcccattc ggacagattc
     2401 gcttccgggc agtttaagct gaatcgttta caaatataat cgtccagccc ctgagatttc
     2461 aacaggcccg gaattttata gatggaatcg acgtctttca gagaaataac cgctttttcc
     2521 ggaacattac agaacaatgc aatcttcgca cgttcgttcg ccggaacagc gcgatctgaa
     2581 cgacaaatca ggatgtcagg ctggataccg atggagagca gctcttttac agagtgctga
     2641 gtcggtttgg ttttgacttc accagacgct gccatgtacg gcaccagcgt caggtgcata
     2701 aacagagtgt gctcacggcc aatttcaaca gccatctggc gaatcgcttc gaggaacggc
     2761 aaggattcga tatcacctac tgtaccgccg atttctacca gtactacgtc atgaccttcg
     2821 ccaccttcca gcacgcgctc tttgattgcg ttagtgatgt gcggaataac ctgcacggtt
     2881 gcgccgaggt agtcaccgcg gcgttcttta cgcagaacgt cagagtagat acgacccgtg
     2941 gtgaagttgt tgcggcggct cattttggta cgaatgaaac gctcgtagtg ccccaggtcc
     3001 aggtcggttt cagcgccgtc ttcagtaacg aacacttccc cgtgttggat tgggctcata
     3061 gtacctggat cgacgttgat gtaaggatcc agtttcatga tggtcacatt gaggccacgg
     3121 gcttcaagaa tggctgcgag ggaggctgcg gcaatgcctt tacccagaga ggatacgacc
     3181 ccgccggtca caaaaatata gttcgttgtc atgctgaacc tgagaagtta ggttgaaaag
     3241 acgatggaat aaccaggacg ggaaagcagt atacccgaac atgacctgtg ccacaaactt
     3301 tcattatccc tcctcttcgc cagcgcacta ttgaaatcag gagtgagaaa atagcccctt
     3361 tggggtaaat gtttttgacg caaatcaagc gcttgtcatt taaaaaatga cacaaatggc
     3421 gcttgaccgc gtaattccct tagagatcaa tttcctgccg ttttacctgt tgccagactt
     3481 cttccattgt ttcgaggtca acacctgtca tttccagtcc acgcgcggca acaatacgct
     3541 ccacttcgcg aaaacgacgc tcaaattttt cgttcgcttt ttgcaatgcg atttccgctt
     3601 ttgtccctaa atgacgagcc agattgaccg tggcaaacag caggtccccc atttcctcct
     3661 ccagtttagc ctggtcgaca acagcctgcc gcgcttcgta catcacctcg tcgatctctt
     3721 cgtagacttt atcgactacc ggaccaagcg tcgtccaatc gaagccaacg ttggcgcaac
     3781 gtttctggat tttttgcgca cgcattaaag ccggtaaact acgaggaata tcgtccagcg
     3841 ccgaatgctg cgctttctgc gcgcgctctt cggttttgat ttgctcccaa cgggcaagca
     3901 cttcactact gttttcggca gaactatcag caaaaacatg cggatggcga cgctctaatt
     3961 tatcgctaat agcagcgcaa atatcattaa agtcaaagcg cccttcttcc tgagccattt
     4021 gcgcgtaaaa caccacctgg aatagcagat cgcccagttc accgcgaaga tcgtcaaaat
     4081 cttcacgggc gatggcgtcc agcacttcgt aggtttcttc aagggtgtaa ggcgcaatgg
     4141 tggcaaatgt ctgctcttta tcccacgggc agccgttttc cggatcgcgc aggcgctgca
     4201 taatagtgag caaacggtcg atttgattca ttgaattgtc ctgaaaattg cgggtctgtc
     4261 aggtggaaac ctgtgaccag aatagaagtg agttagtaac actacccaat cagtacgtta
     4321 attttggctt taatgagttg taattcctct ggggcaactg ttcctttctt cgttgctcct
     4381 cttgcccgcc aggcgatact ttttacctga tcagctaacg ctacgccatc acgttcctga
     4441 ccggataaaa caacttcgaa cggatatcct tttgattgcg ttgtataagg aacacacaga
     4501 cacatacctg ttttgttgtt gtacatgaaa ggactcagga caacagctgg acgatgtcca
     4561 gcttgctcgc taccttttgt cgggtcaaaa tcaacccaaa tcagatcgcc catatcgggt
     4621 acgtatcggc ttaccattac cagacttcct tatctttcgg ctctccccag tcgatattct
     4681 cgtggaggtt ttccggcgtg atgtcgttga ccagttcagc aagcgtaaat acgggctctt
     4741 tacgcactgg ctcaataatt aatttgccat ccaccaggtc aatcttcact tcatcatcaa
     4801 tattcagatt gagcgcctgc attaacgtag ccgggatccg caccgccggt gaatttcccc
     4861 aacgctttac gctactgtgg atcataaccc tttcctcaaa ccgctatcat atgtagatac
     4921 agtatatatc aatctacatt gtagatacga gcaaatttcg gcctaactcc cgtgcaaccg
     4981 acgcgcgtcg ataacatccg gcacctggtt gagtttaccc agcacgcgcc ccagcacttg
     5041 caggttgtaa atctcaatgg tcatgtcgat ggtcgccagt tgctgtttgg tgtcgctacg
     5101 gctggcaacg ccaagcacgt tcaccttctc gttggcgaga atggtcgtga tatcacgtaa
     5161 caacccacta cgatcattag ccaccacgcg gaccaccagc gaatatccgg cggagtagct
     5221 ctcaccccat accgcgtcaa caatgcgttc tggcgcatgg gagcgcagtt ccgccagttg
     5281 ttcgcaatcg gcgcggtgta ctgaaatacc gcgcccctgg gtaatgaagc cgacaatctc
     5341 atctccagga atcggctggc agcagcgcgc gatgtggtgc atcaggttgc caacaccttc
     5401 gaccaccacg cgaccgttat ctttactgcg gttttgcggc gtgtagcttt tttgctgaag
     5461 ttgcttcagc gcggcggcgt cctgctcttc ggcactcggc ttattaaatt gcgattgcag
     5521 gaagttcacc atctgattga gacggatatc cccgccacca atcgccgcca gcaactcgtc
     5581 gacatcatta aagttgtaac gcggcagcag atgtttttct gcttctttca ggctgatccc
     5641 cagatgttcc agctcgtcgt caaggatttg ccgcccagcc agaatgtttt tgtcacggtc
     5701 ctgtttacgg aaccaggcgt gaattttcga acgcccacgg ctggttgtga cgtaaccgag
     5761 gtttgggttt aaccagtcac ggctggggtt cggctgtttc tgggtgataa tttcaatctg
     5821 gtcgcccatc tgcagctggt aggtgaacgg cacaatgcgc ccgccaattt ttgccccgat
     5881 gcagcggtgt ccgacatcac tgtggatgtg gtaagcgaag tccagcggcg ttgatcccgc
     5941 aggcaaatca acgacatcac ctttcggcgt aaagacatac acccggtcgt caaagacctg
     6001 gctgcgtact tcgtcgagca tttcgccgga atcagccatc tcttcctgcc acgcaatcag
     6061 tttacgcagc caggcaatcc ggtcttcatg tcccgaacgt gcgccgccag ctgccgcgcc
     6121 ctctttatat ttccagtgcg cagcaacacc caactctgca tcttcatgca tctgtttggt
     6181 gcggatttgg atctcaacgg ttttgccacc tggccccaga accacggtat gaatagactg
     6241 ataaccgttt ggtttcgggt tagcgacata atcgtcaaac tcatccggca ggtggcgata
     6301 gtgagtgtgc actatcccca gtgcggcata acaatcctgt aaacgctcgg cgacaatacg
     6361 taccgcacgc acatcaaaca gctcatcgaa ggcgaggttc tttttctgca ttttgcgcca
     6421 gatactgtag atgtgtttcg gacgaccata cacttcagct ttaacgcctt cagctttcat
     6481 ctcagcgcgc agatggccga caaactcttc gatatagtgt tcgcggtcga gacggcgttc
     6541 atgcagcagt tttgcgatgc gtttgtattc ggtcgggtgg agataacgga agcagtaatc
     6601 ttccagctcc catttcagtt gcccaatccc aagacggttc gccaacggcg cgtagatatt
     6661 ggtgcactct tttgccgcca gtacgcgttc atcttccggc gcatctttta cttcgcgcag
     6721 atgagcaata cgctccgcca gtttgatgac cacgcagcga aaatcatcga ccatcgccaa
     6781 taacatccgg cgaacgttat cgacctgttc ggaggaaaca gaatcagtgt gcgtcgcttt
     6841 cagctggcgg atcgccgcca tatcacgcac gccgtgaata aggttaacga ccgacttacc
     6901 gacgctctca cgcagcacat cttcgctgac tacgttggca tcagccagag ggaacagcag
     6961 cgccgcccgc agcgtgtcaa tgtccatact taatgtcgag aggatctcca ccatctcaac
     7021 accacgccac aataacagac tggcatccgg atgcccctgc gtctgttgca gacaatacgc
     7081 ccaggtttcg gctaagcact cacacgactt ctggctggta atacccagac ttgcgatcca
     7141 tttttccgga tcaaattcac cagccttatt gatatgtgca cttcttaccg caaccattgt
     7201 cctctccttt agggaccaga cctgccgaaa tcggcaaatc gtaactattt aacgcgcgag
     7261 aaaagtacca tcgattccag atgtcccgtg tgtgggaaca tatccagcat cgccagtcgc
     7321 gcaatggtat atcctgcttt taataacgct tcgctatccc gagccagcgt tgcagggtta
     7381 caggatacat aaactatacg aataggttcc agttttataa tttgctgcat aacacctgcg
     7441 gcacctgctc gcgccgggtc cagcaacact ttatcgaagc cgtttttcgc ccacggctgc
     7501 tttgtgacat cttcttcaag attttcgtga taaaacgtca cattctgtaa accattaaga
     7561 cgcgcattct gctggccttt ttccaccagc gccggaacac cttctacacc gaccacactg
     7621 gctgcttgtt tcgccaatgg cagtgtaaag ttgcccatac cgcagaacag atccagtacg
     7681 cgatcttcag gttgcacatc cagccattcc aacgcacgcg ctaccatttt ttggttcaca
     7741 cccgcattga cctgaataaa atcgcgcggg ctaaaagtta agcgcaaccc gtttgagtca
     7801 taccagggca tctcaccaga gacggtttcg agtatctcac tatcgggggc gagatacaga
     7861 tccaggcctt cagaatgcga aaagcgttcc agtttttcgc gatctgccga acttagcggt
     7921 gcggtatggc gcaaaatcat cagcgtgccg ctggttgcct gtaccagttc aacatgacca
     7981 agatggcgca taacttgtaa gctgcccagg catgccctga ctttgggcag caatgcttca
     8041 agtttgggca ctaaaatggg gcattgttta acgtcgacaa tgtcactgga gcccgctttg
     8101 cgaaagccca tctgaagttg ctgtgttttc ggtaagtagt tcaaacttaa acgcgcgcga
     8161 cggcgatagc cccagggaac atcggcgatc acttcagaga cttcgtgttt cattaatcgg
     8221 gcgagtgccg cacttttgct tcgctgctgt aaatccacgc tggcgtgttg ttgctgacag
     8281 ccaccgcata cgccaaaatg agggcagcgt ggcgtttcgc gttccgggct atcgcttaac
     8341 cggcgcacga ctttagcgcg ggcgtactgt tttttatctt cagtaacagt aacttccgcg
     8401 ttttcctgtg gcagtaatcc aggaataaat aacgctttgc cgttatgtcg cgccacgccc
     8461 tgaccaaaag agtcgaggtc gttgactgaa acggttatga tctgacgcgt cgtcgtgcgt
     8521 cgttttgcag agtagaattg cgccattggc gagactttct caatttaaca gtgtgacctt
     8581 aattgtccca taacggaact ccatgaccaa ctacagcctg cgcgcacgca tgatgattct
     8641 gatcctggca ccgaccgtcc ttattggttt attgctgagt atctttttcg tcgtgcatcg
     8701 ctataacgac ttgcagcgtc aactggaaga tgccggtgcc agcattattg agccgcttgc
     8761 agtttctact gaatatggca tgagcctgca aaatcgcgaa tctatcggtc agttaataag
     8821 cgtactgcat cgtcgccatt ccgatattgt tcgcgcgatt tcggtttatg atgaaaataa
     8881 ccgactcttt gtcacctcca attttcatct tgatccctca tcaatgcagc tcggcagcaa
     8941 cgtgccgttt cctcgccagc tcactgtcac tcgtgacggc gatattatga tcctccgcac
     9001 gccgattatt tctgagagtt actcccccga cgaatcgccc agtagcgatg ccaaaaatag
     9061 tcaaaatatg ttgggatata ttgcgcttga gctggatctt aaatcggttc gcttgcagca
     9121 atataaagag atctttattt ccagcgtgat gatgctgttt tgtatcggta ttgcgcttat
     9181 ttttggctgg cgcttaatgc gcgatgtaac cggtccgatt cgcaacatgg tgaataccgt
     9241 cgaccgcatc cgtcgcgggc aactcgacag ccgggtggaa ggatttatgc tcggcgagct
     9301 ggatatgctg aaaaacggta tcaactcgat ggcaatgtcg ctggctgctt atcacgaaga
     9361 gatgcagcac aatatcgacc aggcgacgtc cgatctgcgt gaaacgctgg agcagatgga
     9421 aattcagaac gttgagttgg atctggcgaa aaagcgcgcc caggaagcgg cgcgtattaa
     9481 atccgagttt ctggcaaata tgtcacacga gctgcgtaca ccactgaatg gtgttattgg
     9541 ctttacccgc ctgacgctga aaacagaatt aacaccaacg cagcgcgatc acctgaatac
     9601 gattgaacgt tcggcaaata atttgctggc aattattaat gatgttctcg acttctcgaa
     9661 actggaagca ggtaagctga ttctggaaag tattccattc ccactacgca gcacgctgga
     9721 tgaagtcgtt actctgctgg cacattcttc tcacgataaa gggttggaac tgacgctcaa
     9781 tattaaaagc gacgtgcctg ataacgtgat cggcgacccg ctgcgattac agcaaatcat
     9841 cactaacctg gtggggaatg caattaaatt caccgagaat ggcaacattg atattctggt
     9901 agaaaaacgt gcgctgagta ataccaaagt gcagattgaa gtgcagattc gggataccgg
     9961 cattggtatt cctgaacgcg atcaatcgcg cttattccag gccttccgac aggctgatgc
    10021 cagtatttcc cgccgtcatg gtggcaccgg gctggggctg gtgattacac aaaaactggt
    10081 taatgaaatg ggcggcgata tttcgttcca tagccagccg aatcgcggtt caactttctg
    10141 gttccacatt aatctcgatc tgaacccgaa cattattatc gaagggccat ccacccagtg
    10201 cctcgcaggt aagcgcctgg cctatgtcga accaaactcc gcagcagcgc aatgcacgct
    10261 ggatatttta agtgaaacgc cgctggaagt ggtttatagc ccaacgttct ccgcgctgcc
    10321 tcccgcgcat tacgacatga tgttgttagg catcgcggtg accttccgcg agccgctaac
    10381 aatgcaacat gagcgattag cgaaagcggt atcgatgacc gatttcctga tgctggcact
    10441 tccttgccat gcacaagtca atgcagaaaa actcaagcaa gatggtatcg gcgcgtgtct
    10501 gctgaaacca ttaacaccta cgcgcctgtt gcctgccctg acggaatttt gtcatcacaa
    10561 acaaaacacg cttttgcctg taaccgatga aagcaagctg gcaatgacag tcatggcagt
    10621 tgatgacaac cccgctaacc tgaaacttat cggcgcattg ctggaagata tggtgcaaca
    10681 tgtggaactt tgcgatagcg ggcatcaggc ggttgaacgg gcgaaacaga tgccgttcga
    10741 tttgatctta atggatattc aaatgcctga catggatggc attcgggcct gcgagctcat
    10801 ccaccagctc ccccatcagc aacaaacgcc ggttatcgcg gtaacggcgc atgcaatggc
    10861 cgggcaaaaa gagaagctgc ttggcgcagg gatgagcgat tatctggcga aaccgattga
    10921 agaagagcga ttgcataatt tgttgttgcg ctacaagcct ggcagcggta tttcctctcg
    10981 cgtcgtgacg cccgaagtca acgaaattgt ggtgaacccg aatgcgaccc tcgactggca
    11041 actggcacta cgccaggcag caggaaaaac cgatttagcg cgcgatatgc tgcaaatgtt
    11101 actcgatttc ctgcctgaag ttcgcaacaa agttgaggaa cagctggttg gagaaaaccc
    11161 ggaaggcctg gttgatttga ttcataaact gcatggcagt tgcggctata gcggtgtacc
    11221 gcgtatgaag aatctctgcc aactgatcga acaacagcta cgtagcggta ctaaagaaga
    11281 agatttggag ccggagctgc tggaactgtt ggacgagatg gataatgtag cgcgcgaagc
    11341 cagcaaaatt ctcgggtaac ggacgtcttg tcgagtagca atcggctttt tatgacgctg
    11401 gtttcagatt ggtgacaaag tgcctgtgtt tatgccggat gcggcgttaa cgccttatcc
    11461 ggcctacaaa accaattaaa ttcaatgaat taaaaaatga tgtagacctg ataaatagca
    11521 caacaggcta ttttgcgttt agcatcagtc tcaagccggc tccagataga gccggttttg
    11581 gttttctgtc ttaacgcacc atgcacgggc gcttgttatc gaacgtccag ccaggaatca
    11641 gatactgcat tcccatcgca tcgtcacgcg cgccaagccc gtgtttctga tacagctcat
    11701 gggctttcat cacttgatcc atatcgattt ctacacccag ccccggtttt tctggcacct
    11761 gtaccagccc gcctttgatc tcaaacggtt ctttggtcag gcgctgatta ccttcctgcc
    11821 agatccagtg cgtatcaata gcagtaattt tacccggtgc agcggcggca acatgggtaa
    11881 acatcgccag ggaaatatcg aagtggttgt ttgagtgtga accccaggtc aggccaaatt
    11941 catggcacat ttgcgccaca cgtaccgaac cttgcatcgt ccagaaatgc ggatccgcca
    12001 gcgggatatc aacggattgc agggagagcg tatggcccat ctgccgccag tcggtggcga
    12061 tcatattggt tgcggtcggc aggccagttg cgcgacggaa ctctgccatc acttcacgcc
    12121 cggagaaacc ttgctccgca ccacacggat cttctgcata agccagcgac cctttcaggt
    12181 atttaccaat tttaatcgct tcgttcagcg accaggcacc gttaggatcg agcgtaatac
    12241 gcgcctgcgg gaagcgttgc gccagtgcca caatagactc ggcctcttct tccccggcca
    12301 gtacaccgcc tttcagtttg aaatcgttga agccatattt ttcatacgcc gcttccgcca
    12361 ggcgcaccac cgcatccggc gtcatcgctt cttcatgacg caggcgatac cagtcgcatg
    12421 agtcatccgg ctggctttga tacggcagcg gtgtggcttt gcgatcaccg acgaagaaca
    12481 gataaccgag catttcgact tcgctacgct gttgaccatc gcccagcagc gatgccacgt
    12541 ttacccccag atgctgcccc agcagatcca gcattgccgc ttctatcccg gtaactacat
    12601 gaatagtggt acgcaaatca aatgtctgca aaccgcgccc accagcatca cgatcggcaa
    12661 aagtattacg caccagcgtc agaacgtttt tgtattcacc cagcgtttta cctaccacca
    12721 gcggaatcgc atcttccagc gttttacgga ttttctcgcc gccgggaatt tcccctacgc
    12781 cagtgtgacc agaattatct ttgataatca caatattacg cgtaaagaac ggtgcgtgtg
    12841 caccactcag attcatcagc atactgtcat gacccgccac cgggatgacc tgcatttcag
    12901 taacaacagg cgtcgtaaat tgagaactca tatttatgtc ctttttcaga atcaatgacg
    12961 gccgaaaacg ggacgtttac ggtcaaaggt ccagccgggg atcaggtact gcatcggacc
    13021 tgcgtcgtta cgcgcaccgc caggcagacg tttataggcc tcatgtgcct tttgtacctg
    13081 ttcccagtcc agttccacgc ccagaccggg cgcatcagga acggcaattt ttccgttttt
    13141 aatctccagc ggatttttgg tcaggcgaca atcgccctcc tgccaaatcc agtgggtatc
    13201 gatagcggta ggattacccg gtgccgccgc gcccacatgg gtaaacatcg ccagagagat
    13261 atcgaaatgg ttattagaat ggcagcccca ggtcagcccc cagtcgtcgc aaagctgcgc
    13321 cacacggact gcaccggaaa gcgtccagaa gtgcggatcg gcaagtggaa tatctaccgc
    13381 attgagcatc accgcatgac ccatttcgcg ccagttggtg gcgatcatgt tagtcgcgac
    13441 gggcaagccg gtcgcccgcc gaaactccgc catcacttca cgaccggaga aaccctgttc
    13501 tgcgccgcat ggatcttcgg cataggtaag aacatcattc agccctttgc atagagaaat
    13561 ggcttcatca agtagccagg caccgttggg atcaacggta atccgcgcat cagggaagcg
    13621 tttcttcaat gcccgaacag tgtcgatttc ttgctcgcca ggtaacaccc cgcccttaag
    13681 tttgaaatct ttaaagccat agcgatcctg tgaggcttcc gccagacgca caacggcttc
    13741 gctgttcatc gctttctgat ggcgcaactg ataccactca tggttgcccg gcgtattttc
    13801 cagataagga agatcggttt tggtccgatc accgatataa aacagataac cgaggacggt
    13861 aatagtctcg cgttgcttgc ctggccccaa cagttcgcag accggaacat tcagcgcctt
    13921 acctagcagg tcaagcaagg cggcttccag cgccgccacg gcattaacgc gcaattcaaa
    13981 agtccaggca cctttgccga aggtatcaaa atcggccgcc tgattaccta tatgcacctg
    14041 ctgaaccact ttattcaggc gcgcaacttc ctggcccagc accatcggaa tagcatcgac
    14101 aagcgtctga taaatcacct ctccgcccgg cgcttcacca ataccggtat gcccggcatt
    14161 atcggtgagt accacaatat tgcgggtgaa atatgcgcta tgtgcaccac caatattaag
    14221 caacatgcta tcgtgcccgg cgaccggaat gactttcata tcagtaataa caggactgga
    14281 ttgtgtcgtc atcttattgc cccgcgacag gtttcagctc gatacgcttg atatcgccca
    14341 ccagcaccag gtagctcagt accgcgatta aggcatgaac accaacataa atcagcgccc
    14401 cattaaagga gccagtcgtg ccaacgatat aaccaattgc gattggcgtg acgatgccag
    14461 aaatgttacc gaacatgttg aacaggccac cggaaagacc gctgatctct tttggcgcgg
    14521 tatctgccat tactgcccaa cccagcgccc cgatgccctt accgaagaag gccagcgcca
    14581 taaagccgat gatcatccac tcaacgttga cgtagttgca gaacaccatc accatcgaca
    14641 gcaacatgcc cattacgatc ggtgttttac gcgcaatgtt cagcgatccc gtgcggcgca
    14701 tcagccagtc ggaaataatc ccacccagca caccgccgat aaaaccgcaa accgccggaa
    14761 cggaagccac aaagcccgct ttcagaatcg acatcccgcg tgcctgcacc agataaaccg
    14821 ggaaccaggt aataaagaag taagtcaggg cgttgataca gtactgaccg atataaacgc
    14881 cgatcatcat ccgtgacccg agcagctgtt tgatctgccc ccacttcacg ctgaacggca
    14941 ctttaacttt ggtgttttgc tgatccatat tgatcagcgc gccacccgcg gcgatatact
    15001 ccagctcttt ctgatttacc cccggatgtt gatttggctc gtggatgact ttcaaccaga
    15061 taaagctgat gacaatcccc agaccgccca taaagaagaa gacgtgtgac cagcccactt
    15121 catgcgtcag ccagcccata atcggcgcga agatcaccgt tgcgaagtat tgagcggagt
    15181 taaaaatcga caccgccgtt cccctttcct gcgccggaaa ccaggccgca acaatgcgac
    15241 tgttgccggg gaaagatggc gcttcagcaa gcccgaccag gaagcgcaga gtaaacaggg
    15301 caacgataat gccgaatcca ctaaagatat cgacgaagcc ttgcagcaag gtaaacatcg
    15361 accagataaa gatcgaccag aagtagacgc gttttgaacc aaaacggtcc agcaaccagc
    15421 caccagggat ctgcccgata acataagccc atgagaaggc agagaacaca tagcccattc
    15481 ccacgggatc aaggccgata tctttggcca tttccgaacc ggcaatagaa agcgtagcgc
    15541 ggtcgccgta gttgaaggat gtgacgataa acaacatcac cactatccag taacgagcat
    15601 tcgtgcgttt ttccacactg ctcgcagcct gacttaaaga actcattgtt gcactcctga
    15661 aaattcgcgt tagccacgct cactctggac tgcgacatag ccaggaaatc agaggtgacg
    15721 tagggtgttt tttgccgttt ttataggtcg ttcgccgaat acggcgcgtg tttatatctg
    15781 gcaatagcag tataaaaagc gcgccatagc ggctcaccgt gcaacaacac aacattaatg
    15841 cgttcaatga ggcccgattt tggcattagc cctggacggt ggaatccact tcacggaaat
    15901 gaaaacaaga acaagaaagg aagggttaaa acgaagaaat aaagaagagt atgaaatgga
    15961 tcgcttgact ccaggcaaac gccagtaaaa atccgcgcta tgaagcagtt tttactggca
    16021 tttgcctgaa aagattcgat tcagcaccgc taaaacgaca tttaccgctc gctgaacata
    16081 tcaggacaac agcgtgcccc actgttcgac ccacggattt gattccgttt ccggttccgg
    16141 gttctcactg gcgtcaatca acagtatttc gccaacccgc tgagcgctct gttcctgcaa
    16201 caaggcatcg aactgtttgc cgccattgca taaatttaca taactgctgt cgccgagcgc
    16261 aatcacgcca taacgcagat tcggctggaa gcccagacta tctttgattc cctgaaagag
    16321 cggcacaatg ctatcaggaa ggtcgccctg cccggtcgtg gacgtaacca ccagaacata
    16381 cttatcctga taaggcagcc agtcgcttaa ttcaggatct tcaaataccg ttgctttgtg
    16441 gccctgtgcg gtcagaattg cttctgcttc ttccgccact aacagcgaat ttccgtacat
    16501 ggtgccgaca aaaataccaa tttccgccat accgttttct ccctggatta ggaacttatc
    16561 tctccatcct gacccgatgg cgcactgaac tcaacccttt cattttcagg aagcagaccg
    16621 cgccagccaa attgtgataa cgcctgcatc caggtgtcgt ccaggcccgc gtgaatagtc
    16681 agcggctcgc cagtaaaagg atgcgtcaat gacaactgac tggcatgtag cattaaccgc
    16741 tggaggccaa aatgctcagc accgctgcga ttctggcgta aatcgccatg tttgctatcg
    16801 ccaataatcg gatgacgcaa atgggcaaga tgtcggcgaa gctgatgttt gcgtccggtt
    16861 ttcggctcca gttccaccag gccgtagcgc gtggtcgggt aacgtccggt cgctaccggc
    16921 atttctacgg tcgccagacc gcgataatgc gtcactgctg gctgcgagcc tttatcttcg
    16981 cgggcaaatt tatcagcgat tttgtccagt tcttccacca gtggataatc cagaactgct
    17041 tcttccatca accagccgcg cacaatcgca tggtaacgtt tctggatttg gtgctgttca
    17101 aactgttgtg ccagcagccg tccggcctcg ctggataatc ccatcaacaa cacaccagaa
    17161 gtgggtcggt ccagacgatg agcggtaaaa acgtgctggc caatctgatc acgcacggtt
    17221 tgcatgacca ctactttctc gtcgcgatcc agccagctgc ggtgaaccag ccagccggag
    17281 ggtttattta ccgcaaccag ccattcatcc tgatagagta tttccagcat tagctcgcat
    17341 catccgcaaa aagagcatcc agtttttcca gctcagccag aataagcgcg cgttgcggat
    17401 ggtccgtcgc cagcgccatt tcataatagg gtgcaacggc aaaagcgccc ggtaacggct
    17461 gtttgttatc taacaaatcg tgcattcgcg ggatcagcac ccactgcaac cactccagtg
    17521 gttccatggt gtccataaag aacggttggg tactattaaa ttgatgcggc tggggttcat
    17581 cgtttcgcca gtgctgatgt tcacgcagta acgcttcaag cgcctgcaac tggagacgaa
    17641 cgcggtcatg agtggtcata aagcaacctc aataagaaaa actgccgcga aggatagcag
    17701 gaataaaaaa agggagcact gtattcacag cgctcccggt tcgtttcgca gcattccagc
    17761 tacttttgtt gctccctgct catccttgac aacttttcct ctggccttgc ggccaatcgt
    17821 tcatcctgaa ctattgcttc ctgctcacac caccccgatg tgatacttca tcctgaagtg
    17881 tccctggcct tcctgaccca ccgaatcatc ctgaccggtt ctcattctcc atcctggagg
    17941 tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc cagaagcctt tccctgaaac
    18001 accatcctgg tgtgtcctgc agaagtgtca tcatcctgat gttcacttcg ttgtctgact
    18061 ccctgtcgac gaagatagaa tcgtcttttt cgcgaagtct tacaaggcgc ttacagacaa
    18121 tgccttaagg aaaatttcat atgaaagtta agaattaaca atcaacattt tgattttaaa
    18181 tgaaaataac gattcttatc tttttaagta ttcgcttatt tcctggcgat ctcctacaga
    18241 gtctgtaaga gatctctcac aaccagatgg ggtatttgga ttacagaaga ggcttaagtt
    18301 gattaaggaa ttccgcaaga tttgaggcta aatgcgtccg tttacgtgtt cccagcgtct
    18361 ctttgcacac ttcaccggag agattacaca ccgagatgac ctctaactct tcttccagcg
    18421 tcgcgataaa tagcgtgggc ggcagtttta gccttttctg agtcaccaga tgaccaatca
    18481 gattttcctg cacccgacgg aagtcgtctt cactccaggt ttgcagcaat gtcagtttga
    18541 tatcgccaaa ctgcgcgtgc atatccccgg caaactgagt cgtataaaag gtatgaattg
    18601 tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt tacattttgt tccccggtaa
    18661 acggctgggg ttgccagtat acggcatctt cggtagttga aataatgcac ggtgaaggaa
    18721 caccgtacag ttcctcactt aacggccagc ttttatgctc ttcatgccac gcatcgcagt
    18781 agcgtgcagt aaaatctttc agggcttgtg cggtcaaatc gtccactaat ttctctcttc
    18841 acttaaacca gatacacttg tcgtttagtt tatctggttt atgacggtga aacatgtctt
    18901 cttatgcaaa ccatcaggca cttgcgggcc tgactcttgg aaaatcaacc gattaccggg
    18961 atacctatga cgccagccta ctgcaaggcg ttccacgcag cctgaatcgc gacccgctgg
    19021 gtctgaaagc ggataacctg ccttttcacg gtacggatat ctggacgctg tatgaacttt
    19081 cctggctgaa tgcgaaaggt ttgccgcagg tcgctgtcgg tcatgttgaa cttgattaca
    19141 ccagcgtaaa tctgattgag tcgaagagtt ttaagctcta tctcaacagt tttaaccaga
    19201 cgcgttttaa taactgggat gaggtgcgcc agacgctgga gcgcgactta agcacttgcg
    19261 cccagggtaa ggttagcgtg gcgttatatc gtcttgatga actggaaggc cagccgatag
    19321 gtcatttcaa tggcacctgc attgatgacc aggatatcac tatcgataac tatgaattca
    19381 ctactgacta tctggagaat gccaccagcg gtgaaaaagt agtggaagag acgcttgtca
    19441 gccacctgct gaaatcaaac tgcctgatca cccatcaacc agattggggt tcgatccaaa
    19501 ttcagtatcg tggacgccaa attgacagag aaaaactgct gcgctacctg gtctcattcc
    19561 gtcatcacaa cgagttccac gaacagtgcg tggaacgcat ctttaatgac ctgttacgct
    19621 tctgccagcc agagaaatta agcgtttacg cacgttatac ccgtcgtggc ggtctggaca
    19681 ttaacccgtg gcgcagtaat agcgattttg tcccatcgac cacaagactg gttcggcaat
    19741 aaattttttc tcaattttgc gtgctggatt cacgcagaag gttgtgaaag gtcatcaggc
    19801 agggctattg taatcaaagg gaatgacgat attcgtccca taaggagttt ttcttgatta
    19861 cacatattag cccgcttggc tccatggata tgttgtcgca gctggaagtg gatatgctta
    19921 aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa ctgttcactt gccgtactga
    19981 actccggtag tttgaccgat aacagcaaag aattgctgtc tcgttttgaa aatttcgata
    20041 ttaacgtcct gcgccgtgaa cgcggcgtaa agctggaact gattaatccc ccggaagagg
    20101 cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa cttgttcgcg gttctgcgag
    20161 acattctctt cgtttacggg caaatccata ataccgttcg ttttcccaac ctgaatctcg
    20221 acaactccgt ccacatcact aacctggtct tttccatctt gcgtaacgct cgcgcgctgc
    20281 atgtgggtga agcgccaaat atggtggtct gctggggcgg tcactcaatt aacgaaaacg
    20341 agtatttgta tgcccgtcgc gtcggaaacc agctgggcct gcgtgagctg aatatctgca
    20401 ccggctgtgg tccgggagcg atggaagcgc cgatgaaagg tgctgcggtc ggacacgcgc
    20461 agcagcgtta caaagacagt cgttttattg gtatgacaga gccgtcgatt atcgccgctg
    20521 aaccgcctaa cccgctggtc aacgaattga tcatcatgcc ggatatcgaa aaacgtctgg
    20581 aagcgtttgt ccgtatcgct cacggcatca ttatcttccc tggaggtgtg ggtacggcag
    20641 aagagttgct gtatttgctg ggaattttaa tgaacccggc caacaaagat caggttttac
    20701 cattgatcct caccggcccg aaagagagcg ccgactactt ccgcgtactg gacgagtttg
    20761 tcgtacatac gctgggcgaa aacgcgcgcc gccattaccg catcatcatt gatgacgccg
    20821 ctgaagtcgc tcgtcagatg aaaaaatcga tgccgctggt gaaagaaaat cgccgcgaca
    20881 ctggtgacgc ttacagcttt aactggtcaa tgcgcattgc accagatttg caaatgccgt
    20941 ttgagccgtc tcacgagaat atggctaatc tgaagcttta cccggatcaa cctgttgaag
    21001 tgctggctgc cgacctgcgc cgtgcgttct ccggtattgt ggcgggtaac gtaaaagaag
    21061 tcggtattcg cgccattgaa gagtttggtc cttacaaaat caacggcgat aaagagatta
    21121 tgcgtcgtat ggacgacctg ctacagggtt ttgttgccca gcatcgtatg aagttgccag
    21181 gctcagccta catcccttgc tacgaaatct gcacgtaatc tccgctcccc cggtgacgtt
    21241 tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat ttgttatatg
    21301 aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgtc atacaattta
    21361 tgttgcaacg caaacgtttc cctattttca taaaccgtat tttatcgcta tgaaaaagaa
    21421 attatcgcca tgattaacta a
//
LOCUS       DAZFRN010000080        21262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFRN010000080 DAZFRN010000000
VERSION     DAZFRN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.080"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(55..1017)
                     /gene="pfkA"
                     /locus_tag="ACHKUB_002871"
     CDS             complement(55..1017)
                     /gene="pfkA"
                     /locus_tag="ACHKUB_002871"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO6655855.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(1198..2100)
                     /gene="fieF"
                     /locus_tag="ACHKUB_002872"
     CDS             complement(1198..2100)
                     /gene="fieF"
                     /locus_tag="ACHKUB_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO6655856.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(2249..2749)
                     /gene="cpxP"
                     /locus_tag="ACHKUB_002873"
     CDS             complement(2249..2749)
                     /gene="cpxP"
                     /locus_tag="ACHKUB_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO6655857.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            2899..3597
                     /gene="cpxR"
                     /locus_tag="ACHKUB_002874"
     CDS             2899..3597
                     /gene="cpxR"
                     /locus_tag="ACHKUB_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO6655858.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            3594..4967
                     /gene="cpxA"
                     /locus_tag="ACHKUB_002875"
     CDS             3594..4967
                     /gene="cpxA"
                     /locus_tag="ACHKUB_002875"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO6655859.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(5073..5747)
                     /gene="yiiM"
                     /locus_tag="ACHKUB_002876"
     CDS             complement(5073..5747)
                     /gene="yiiM"
                     /locus_tag="ACHKUB_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO6655860.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(5896..6879)
                     /gene="kdgT"
                     /locus_tag="ACHKUB_002877"
     CDS             complement(5896..6879)
                     /gene="kdgT"
                     /locus_tag="ACHKUB_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO6655861.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(7139..7759)
                     /gene="sodA"
                     /locus_tag="ACHKUB_002878"
     CDS             complement(7139..7759)
                     /gene="sodA"
                     /locus_tag="ACHKUB_002878"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO6655862.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            8044..9078
                     /gene="rhaT"
                     /locus_tag="ACHKUB_002879"
     CDS             8044..9078
                     /gene="rhaT"
                     /locus_tag="ACHKUB_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO6655863.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(9075..9923)
                     /gene="rhaR"
                     /locus_tag="ACHKUB_002880"
     CDS             complement(9075..9923)
                     /gene="rhaR"
                     /locus_tag="ACHKUB_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO6655864.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(9997..10833)
                     /gene="rhaS"
                     /locus_tag="ACHKUB_002881"
     CDS             complement(9997..10833)
                     /gene="rhaS"
                     /locus_tag="ACHKUB_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO6655865.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            11121..12590
                     /gene="rhaB"
                     /locus_tag="ACHKUB_002882"
     CDS             11121..12590
                     /gene="rhaB"
                     /locus_tag="ACHKUB_002882"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO6655866.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            12587..13846
                     /gene="rhaA"
                     /locus_tag="ACHKUB_002883"
     CDS             12587..13846
                     /gene="rhaA"
                     /locus_tag="ACHKUB_002883"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO6655867.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            14021..14845
                     /gene="rhaD"
                     /locus_tag="ACHKUB_002884"
     CDS             14021..14845
                     /gene="rhaD"
                     /locus_tag="ACHKUB_002884"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO6655868.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            14855..15169
                     /gene="rhaM"
                     /locus_tag="ACHKUB_002885"
     CDS             14855..15169
                     /gene="rhaM"
                     /locus_tag="ACHKUB_002885"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO6655869.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            15482..15928
                     /gene="frvA"
                     /locus_tag="ACHKUB_002886"
     CDS             15482..15928
                     /gene="frvA"
                     /locus_tag="ACHKUB_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO6655870.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            15939..17390
                     /gene="frvB"
                     /locus_tag="ACHKUB_002887"
     CDS             15939..17390
                     /gene="frvB"
                     /locus_tag="ACHKUB_002887"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO6655871.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYVLAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            17380..18450
                     /gene="frvX"
                     /locus_tag="ACHKUB_002888"
     CDS             17380..18450
                     /gene="frvX"
                     /locus_tag="ACHKUB_002888"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6655872.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            18450..20198
                     /gene="frvR"
                     /locus_tag="ACHKUB_002889"
     CDS             18450..20198
                     /gene="frvR"
                     /locus_tag="ACHKUB_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO6655873.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDIWPQYPGNEIKQIV
                     EQCGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(20248..21261)
                     /gene="yiiG"
                     /locus_tag="ACHKUB_002890"
     CDS             complement(20248..21261)
                     /gene="yiiG"
                     /locus_tag="ACHKUB_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO6655874.1"
                     /translation="MIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEA
                     KPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERT
                     VYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYT
                     QENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAELKNIEEREGK
                     TFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFIN
                     SAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVDDSFPRALREYNEMVDDYN
                     SLR"
BASE COUNT         5117 a         5541 c         5443 g         5161 t
ORIGIN      
        1 ttttgcttct gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata
       61 cagttttttc gcgcagtcca gccagtcgcc tttgaacgga cgcttcatgt tttcgatagc
      121 gtcgatgatg tcatggtgaa ccagctgttc gttctggata cctacgcaac gaccgccgta
      181 acctgccagc agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta
      241 aggcaccgga gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc
      301 ggtttctttc tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc
      361 cacgatcgcg tgttttttac ctttcgcgat acccgctttg atttcgttta ccagatcttc
      421 acggctgaat tcaacttccg gaaccacaac gaattcacag ccaccggcaa tggccgcagc
      481 caacgtcaga tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga
      541 agaagaggtg tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa
      601 accgatagtg tagtcagtgc ctttgatgtc gttgtcgata gtgccaggca gaccgatgca
      661 cgggaagccc atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac
      721 caccagcgcg tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc
      781 gtcgcggaat tccgggaaac gcgcagaacc gaggaacgta ccaccacggt tgatcatgtc
      841 agaaacgctg taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc
      901 ataaatgccc attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc
      961 ggcgttcatg cctggcgcat caccgccgct tgtcaacaca ccgattttct taatcatgac
     1021 tacctctgaa ctttggaatg caaaataaaa tctgttgccg gaagtcttct tgcacatcga
     1081 agtgatccaa cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc
     1141 aggccaaatg gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta
     1201 tgaaagcata gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat
     1261 tacatccgat cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg
     1321 tgcctgaacc aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg
     1381 ccctgactgc cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat
     1441 atcaataatt tcttgccgtt cctcatcagg caatgcgcga tccagtaatg actgtaccgc
     1501 ctcatatccc atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag
     1561 agcatcggcg cgatgccagc cgtaccagga caaccccagc gccagcagaa ttgcgccgtt
     1621 catcataaca tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg
     1681 gcgcaccacc caacgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt
     1741 cacgataacc ccgacgcctg gatctgtcat cggtgttgga gatatcagat gttgaatacc
     1801 cgtcaaaaac aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag
     1861 ggactctgct ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg
     1921 caccaccagt aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc
     1981 gagaatactc accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga
     2041 agccatcgcc gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat
     2101 aaatactccc gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta
     2161 agcagcaggc aaattgagga taaaaaaaac ccccacatca tgtgggggaa gacagggatg
     2221 gtgtctatgg caaggaaaac agggtttact actgggaacg tgagttgcta ctactcaata
     2281 gcttcaacga tgaacttttt tgccattgcg tcacgtcacg caactgctcc attcgttgtt
     2341 gatgtttctc gtttaaaacc gcttgctgct ccggcgttaa caggcgatac atttggttgc
     2401 ggacttttgc catctcaacc tgacgagcaa tttgctcatt cgccattttt tctgcctgtg
     2461 cgcgcacagc gttttcatca aaattttctg cggtgacaag gcgatgcatt gtctccagtt
     2521 cgctaacatt aacaggaggc tgttcgtgcc gggcctgttg cataagatct cgcatctgct
     2581 gacgctgatg ttcggttaaa cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg
     2641 taagttcttc acccggatgc cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg
     2701 aactgactgc cagcgttgag gccatgacgg cagcggtaac tatgcgcatc atttgctccc
     2761 aaaatctttc tgtcgcgatt caacgataga gagtttacga ttcaggctgc aaacatgcgt
     2821 cagggggtgt aaaacaacgt aaagtcatgg attagcgacg tctgatgacg taatttctgc
     2881 ctcggaggta tttaaacaat gaataaaatc ctgttagttg atgatgaccg agagctgact
     2941 tccctattaa aggagctgct cgagatggaa ggcttcaacg tgattgttgc ccacgatggg
     3001 gaacaggcgc ttgatcttct ggacgacagc attgatttac ttttgcttga cgtaatgatg
     3061 ccgaagaaaa atggtatcga cacattaaaa gcacttcgcc agacacacca gacgcctgtc
     3121 attatgttga cggcgcgcgg cagtgaactt gatcgcgttc tcggccttga gctgggcgca
     3181 gatgactatc tcccgaaacc atttaatgat cgtgagctgg tggcacgtat tcgcgcgatc
     3241 ctgcgccgtt cgcactggag cgagcaacag caaaacaacg acaacggttc accgacactg
     3301 gaagttgatg ccttagtgct gaatccaggc cgtcaggaag ccagcttcga cggacaaacg
     3361 ctggagttaa ccggcactga gtttaccctg ctctatttgc tggcacagca tctgggtcag
     3421 gtggtttccc gtgaacattt aagccaggaa gtgctgggca aacgcctgac gcctttcgac
     3481 cgcgctatcg atatgcacat ttccaacctg cgtcgtaaac tgccggatcg taaagatggt
     3541 cacccgtggt ttaaaacctt gcgtggtcgc ggctatctga tggtttctgc ttcatgatag
     3601 gcagcttaac cgcgcgcatc ttcgccatct tctggctgac gctggcgctg gtgttgatgt
     3661 tggttttgat gttacccaag ctcgattcac gccagatgac cgagcttctg gatagcgaac
     3721 agcgtcaggg gctgatgatt gagcagcatg tcgaagcgga gctggcgaac gatccgccca
     3781 acgatttaat gtggtggcgg cgtctgttcc gggcgattga taagtgggca ccgccaggac
     3841 agcgtttgtt attggtgacc accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc
     3901 agatcattcg taactttatt ggtcaggccg ataacgccga tcatccgcag aagaaaaagt
     3961 atggccgcgt ggaactggtc ggtccgttct ccgtgcgtga tggcgaagat aattaccaac
     4021 tttatctgat tcgtccggcc agcagttctc aatccgattt cattaactta ctgtttgacc
     4081 gcccgctatt actgctgatt gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg
     4141 cctggagtct ggcaaaaccg gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg
     4201 gaaacttacg ccagcacccg gaactggaag cggggccaca ggaattcctt gccgcaggtg
     4261 ccagttttaa ccagatggtc accgcgctgg agcgcatgat gacctctcag cagcgtctgc
     4321 tttctgatat ctctcacgag ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt
     4381 tactgcgccg tcgtagtggt gaaagcaagg aactggagcg tattgaaacc gaagcgcaac
     4441 gtctggacag catgatcaac gatctgttgg tgatgtcccg taatcagcaa aaaaacgcgc
     4501 tggttagcga aaccatcaaa gccaaccagt tgtggagtga agtgctggat aacgcggcgt
     4561 tcgaagccga gcagatgggc aagtcgttga cggttaactt cccgcctggg ccgtggccgc
     4621 tgtacggcaa tccgaacgca ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc
     4681 gttattccca tacgaagatt gaagtgggct ttgcggtaga taaagacggt atcaccatta
     4741 cggtggacga cgatggtcct ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt
     4801 tctatcggac cgatgaagca cgcgatcgtg aatctggcgg tacaggattg gggctggcga
     4861 ttgttgaaac cgccattcag cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg
     4921 gcggtttacg gctggtgatt tggttgccgc tgtataagcg gagttaaact ccgcatttgt
     4981 aggcaggata aggcgtttac gccgcatccg gcatttgagc aggatgcctg atgcgacgct
     5041 gatagcgtct tatcaggcct acactccggg ggttattttc cccacaaccg gcgggaaaaa
     5101 tcttcgatct tgccgctcag tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac
     5161 ccggcggcgg agagtaaacg gtgatattga tcgtcatcaa acggcatatg ccatgcgatg
     5221 gcggcagcct cctgcacggt cacatcgctg acacgggaaa ccaactccag cggcgcgtcc
     5281 gcagacactt tccctggcgc tatcacgcta tacaaccacc ccaccttacc tgtgttttgc
     5341 atcaactgcg ctatatcgct gatatcaaaa tggtaattga gtttatagca aggcgagcgc
     5401 ggctggctga cctgaattaa tgcctctccc cagcggaaaa tatcgcccat ataaacatta
     5461 ctttccgtga gaccgtcggt tgagagattt tcgccaaacg caggcgcaac aaacaactcc
     5521 gcctgttccg gaaattcccg cgcccagtag agataatgct cacgaggata atgacacagc
     5581 gctctgtctg gtccgccgtg aacctttttc tccgcctgct cgtcaccttc cagccccagc
     5641 tccgtcagca tcaactcacc atcaacctgg attttggcaa ttgcgctggg tttgccttcg
     5701 ggataagcct gaatcttgcc tgtgtataca tcaaccggat atcgcatctg ttccctctcc
     5761 acgagaaatt tccccattag accatattta ttttatgtca ggcgtgctgt tttcgcggta
     5821 agtatccagg caggaactca atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg
     5881 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
     5941 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
     6001 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
     6061 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
     6121 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
     6181 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
     6241 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
     6301 ttctcgtaat tcagggtcaa ggttccccag ggcaaagccc accaggaacg gtaatactgc
     6361 gccgacgaaa acatgcggtt caaacgaggc aatccctgca gtgcccagaa taatcatcgt
     6421 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt cttttgtgcc
     6481 gtactgctgc atgatggaag cgtaaagtcc gccattggtc atatccatcg ccgccaccag
     6541 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
     6601 cgaggcaatt gccgcaacca cccacgcgac ggcaattttt gttaccacca gcgtaccgga
     6661 tttacgcaat accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
     6721 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
     6781 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
     6841 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     6901 ttttaagggc tgatgcgtca tttctcaatg gagcatcagt gttgatggaa acgaagatag
     6961 acattcattt aatcaataaa agtgatcaaa accacatcaa ttgaaacact gttttatttt
     7021 tatcggatca ttgtttttat tttgaaattg atcacaaaaa aaccaccgcc gttggcgatg
     7081 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     7141 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     7201 taatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     7261 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagt ggagaatcct
     7321 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     7381 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     7441 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     7501 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt tcgcagtacg gttttcttgt
     7561 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     7621 gcaggctttc cagcgccgcg ttggcgttgt tgacgtaggt ctgatggtgt ttggtgtggt
     7681 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     7741 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     7801 cgtaagcagt tagttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     7861 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     7921 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     7981 gcacgatttt tacactcatc tcgtcggaga tgtgacgcga cgaaaaatga tgaggataag
     8041 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     8101 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     8161 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     8221 aatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     8281 ggcgctatgt gggggatcgg taatatcaac tacggcctga ccatgcgtta tctcggcatg
     8341 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     8401 attatcaatg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttgctc
     8461 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     8521 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     8581 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     8641 gaagccgctg ccgcactagg cgtcgatcca ctgtatgtcg ctctgccaag ctatgtcatc
     8701 atcatgggcg gcggcgcgat catcaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     8761 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     8821 ttactctcag cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctggggc
     8881 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     8941 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatgc aggtcgccgt
     9001 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgccaa catcgtcggc
     9061 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     9121 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     9181 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     9241 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     9301 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     9361 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     9421 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     9481 gccatttcgt tagcaaacga cacatgctga ctactttcat gctcaagctg accgataacc
     9541 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     9601 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     9661 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     9721 atgtaaaaga gatcgccacg ggtaatgcga taagggcgat cgttgagtac atgcagacca
     9781 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaaaacatct
     9841 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     9901 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     9961 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaaac catcccgtcc
    10021 ctgacgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
    10081 actgtcgctg aatccacagc gataggcgat atcagtaacg ctggcctcgc tgtggcgtag
    10141 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtaa cgctgaggcg tcagtcccgt
    10201 ttgctgctta agctgccgat gtagcgtacg cagcgaaaga gaaaattgct ccgccacggc
    10261 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
    10321 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
    10381 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
    10441 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtggga
    10501 aggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
    10561 tcgatccggc gagcgataca gcacattagt cagacacaga ttatcggtat gttcatacag
    10621 atgccgatca tggtcgcgca caaaacagac cgtgccaccg gtgatggtat agggctgccc
    10681 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
    10741 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttaccaga
    10801 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
    10861 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
    10921 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
    10981 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
    11041 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
    11101 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
    11161 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
    11221 aaatccatcg ttttaacaat gggctgcata gtcagaacgg ctatgtcacc tgggatgtgg
    11281 atagcctgga aagtgccatt cgccttggat taaacaaagt gtgtgaggaa gggattcgta
    11341 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
    11401 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atggcgcagg
    11461 cacaacaaca actcggcaaa cgcgatattt atcaacgtag cggcatccag tttctgccct
    11521 tcaatacgct ttatcagtta cgggcgctga cggagcaaca acctgaactt attccacaca
    11581 ttgctcacgc tctgctaatg ccggattatt ttagttatcg ccttaccggc aagatgaact
    11641 gggagtacac caacgccaca accacgcaac tggtcaatat caatagcgac gactgggacg
    11701 agtcgctact ggcgtggagc ggtgccaaca aagcctggtt tggtcgcccg acgcatccgg
    11761 gtaatgtcat aggtcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
    11821 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
    11881 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
    11941 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
    12001 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
    12061 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
    12121 ccaatgacga tcgctttatt aatcctgagg cgatgtgcag cgaaattcag gctgcgtgtc
    12181 gggaaacggc gcaaccgatc ccggaaagtg atgctgaact ggcgcgctgt attttcgaca
    12241 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
    12301 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
    12361 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
    12421 tcggcatcca gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
    12481 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
    12541 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
    12601 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
    12661 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
    12721 ttccggtttt gaaaacccgg aaggttcgct gaccggtggg attcaagcta ctggtaatta
    12781 tccgggcaaa gcgcgtaatg ccagtgagct acgtaccgat ctggaacagg ctatgcggct
    12841 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
    12901 ctcgcgcgat cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
    12961 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
    13021 tacgctttcc catgccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
    13081 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
    13141 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
    13201 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
    13261 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
    13321 catggggtat gccaccagcc gccagactgc gctatgcctg gacgccgggc acttccaccc
    13381 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtgccgc agttgctgct
    13441 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
    13501 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
    13561 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
    13621 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
    13681 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgtggca
    13741 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
    13801 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
    13861 ggcacgagcg ccttatccgg cctacgggtc ggcaacagtt gtaggcctga taagacgcga
    13921 cagcgtcgca tcaggcattg attgccggat gcggcacgag cgccttatcc ggcctacggg
    13981 tcgtgcatct gacaacaccg aatttacagg aacacagaac atgcaaaaca ttacccagtc
    14041 ctggtttgtc cagggaatga tcaaagccac caccgatgcc tggctgaaag gctgggacga
    14101 gcgcaacggc ggcaacctga cgctgcgcct ggatgacgcc gatatcgcgc tttatcacga
    14161 caacttccac ccacaaccgc gctatattcc gctcagccag cccatgcctt tactggcaaa
    14221 tacgccgttt attgtcaccg gctccggcaa attcttccgt aacgtccagc ttgatcctgc
    14281 agctaactta ggcgtcgtaa aagtcgacag cgacggcgcg ggctaccata ttctctgggg
    14341 gttaaccaac gaagccgttc ccacttccga acttccggct cacttccttt cccactgcga
    14401 gcgcattaaa gccaccaacg gtaaagatcg ggtgatcatg cactgccacg ccaccaacct
    14461 gatcgccctc acctatgtac tggaaaacga caccgcggtc tttactcgcc aactgtggga
    14521 aggcagcacc gagtgtctgg tggtattccc ggatggcgtt ggcattctgc cgtggatggt
    14581 gcccggcacg gacgaaatcg gccaggcgac cgcacaagag atgcaaaaac attcgctggt
    14641 gttgtggccc ttccacggcg tcttcggcag cgggtcgacg ttggatgaaa cattcggttt
    14701 aatcgacacc gcagaaaaat cagcacaagt attagtgaag gtttattcga tgggcggcat
    14761 gaaacagacc atcagccgtg aagagttgat agcgctcggc aagcgcttcg gcgttacacc
    14821 actagccagt gcgctggcgc tgtaaggagc aaacatgatc cgcaaagcct ttgtcatgca
    14881 ggttaacccc gacgctcacg aagagtatca gcgtcggcat aaccccatct ggccggaact
    14941 ggaagcagtg ttgaaatctc acggtgcgca taactacgcc atttacctcg acaaagcgcg
    15001 taatctgctg tttgccacgg tagagattga atctgaagag cgctggaatg cggttgccag
    15061 tactgatgtt tgccaacgtt ggtggaaata tatgaccgac gtcatgcccg cgaacccgga
    15121 taacagcccg gtgagtagcg aactgcaaga agtgttttac ctgccgtaat tgcctaatgt
    15181 gccggagacg cctccggcac aatattttta atttcgattt gaaaaataaa catattacac
    15241 cttcagttaa tatatcccgg taacttatat ttccccgatg cattgtgagt aaatcacaaa
    15301 aataatgaat aactcattaa tgattcatgt ggtttattta aataaccatt atgtgcatta
    15361 ctccgcaaat ctgacctttc actctgtctc aaattgtcat tgtttaccga catatcgcct
    15421 ctcatcactt aatcgctcca ccattacaat tgattaaaat atatttcatg gagtccgatt
    15481 aatggcagct cttactgcaa gctgtattga cctgaatatt cagggcaatg gcgcttattc
    15541 cattctgaag cagttggcga caatggcgtt acaaaacggt tttatcaccg actcacacca
    15601 gttcctgcaa accctgctgc tgcgcgaaaa aatgcactct actggatttg gttccggcat
    15661 cgccgtgccg cacggtaaaa gcgcctgcgt taaacaaccg ttcgtattat tcgcccgcaa
    15721 agcgcaggct attgactgga aagccagcga tggcgaagac gtcaattgct ggatctgcct
    15781 cggcgtgccg caaagcggcg aagaggatca ggtcaaaatc atcggcacac tgtgtcgcaa
    15841 aattattcac aaggaattta ttcatcaact gcaacagggc gataccgacc aggtgcttgc
    15901 cttgttaaat caaaccctca gctcataagg aagtggcgat ggagtcatcc ttacgtattg
    15961 tcgcgatcac caactgcccc gccgggatcg ctcacaccta catggtggcg gaagccctgg
    16021 aacagaaagc ccgttctctc ggtcatacca taaaagtgga aactcaaggg tccagtggcg
    16081 ttgaaaaccg cttatccagc gaagagattg ccgctgccga ttacgtcatt ctcgctaccg
    16141 ggcgtggcct gagcggcgat gatcgcgcgc ggtttgccgg gaagaaagtt tatgagattg
    16201 ccatctccca ggcgttgaaa aatatcgacc agattttcag cgaattaccg acaaactcgc
    16261 agctttttgc cgcagatagc ggcgtgaagc ttggcaaaca ggaagtgcaa agcggcagcg
    16321 tgatgagcca cctgatggcg ggcgtctctg cggcgctgcc gtttgtcatc ggcggtggta
    16381 tcctggtggc gcttgccaac atgctggtgc agttcggttt gccgtatacc gatatgtcga
    16441 aaggcgcgcc gtcgtttacc tgggtagttg aatccatcgg ttaccttggc ttcaccttta
    16501 tgatccccat catgggcgct tatattgcct cgtcgattgc cgataaaccg gctttcgctc
    16561 cggcgtttct ggtttgctat ctggcgaacg acaaagcgct gctcggcacc cagtcgggtg
    16621 cgggattcct cggcgcggtg gtgctggggc tggcgattgg ctatttcgtc ttctggttcc
    16681 gcaaagtgcg ccttggcaaa gcattacaac cgctgctcgg ttccatgctg atcccgttcg
    16741 ttactctgct ggttttcggc gtgctcactt actacgttat cgggccggtg atgtccgacc
    16801 tcatgggcgg gctgctccac ttcctgaata ccattcctcc atcaatgaag tttgcggcag
    16861 cgtttctggt cggtgcgatg ctggcgttcg atatgggcgg ccccatcaat aaaaccgcct
    16921 ggttcttctg cttctcactg ctggaaaaac acatttatga ctggtacgcc atcgtcggcg
    16981 ttgtcgcgtt gatgccgccc gttgcggcgg gtcttgcgac gttcattgcg cccaaactgt
    17041 ttacccgaca ggaaaaagag gctgccagta gtgccattgt ggtcggtgcc acagtcgcaa
    17101 ccgaaccggc tataccttac gttctggcag ctccgttgcc gatgattacc gccaatacgc
    17161 tggcgggtgg cattaccggc gtgctggtga tcgctttcgg aatcaaacgt ctggcacctg
    17221 gtttgggtat ctttgacccg ctgatcggcc tgatgtcgcc ggtgggttcg ttttatctgg
    17281 tgctggcgat tggcctcgcg ctgaacattt cattcattat cgtcctgaaa ggattgtggt
    17341 tacgccgcaa agcgaaagcc gcgcaacagg agcttgtcca tgaacattga gttactgcaa
    17401 cagttgtgcg aagccagcgc cgtcagcggc gatgaacagg aagttcgcga cattctgata
    17461 aacacgctgg aaccttgcgt taatgagatc acctttgatg gtctgggcag ctttgttgcc
    17521 cgtaagggga ataaaggtcc aaaagttgcc gttgtcgggc atatggatga agtcggcttt
    17581 atggtcaccc acatcgacga gagcggtttt ctgcgtttca ccaccattgg tggctggtgg
    17641 aatcagtcaa tgctcaacca ccgggtaacc atacgcacac acaagggatt taaaatccct
    17701 ggtgtgattg gttccgtcgc acctcatgcg ttaacggaaa agcaaaagca acagccgctg
    17761 tcatttgatg agatgttcat tgatattggc gcgaacagtc gcgatgaggt ggaaaaacgc
    17821 ggtgtggcga tgggcgattt tattagcccg gaagccaatt ttgcctgctg gggcgaagat
    17881 aaagtggtcg gcaaggcgct ggataaccgc atcggctgcg cgatgatggc ggagctacta
    17941 cagacagtaa ataacccaga aattacgctg tacggcgttg gcagtgtgga agaagaagtt
    18001 gggctacgcg gggcacaaac ctcggcggaa cacattaaac cggatgtggt gattgtgctg
    18061 gataccgctg tcgcaggtga tgttccgggc attgataaca ttaaataccc gctgaaactg
    18121 ggccaggggc cggggctgat gctgtttgac aagcgctact tccccaacca gaaactggta
    18181 gcagtgttaa aaaactgtgc cgcacataac gatttaccgc tgcaattttc caccatgaaa
    18241 accggagcga cggatggcgg gcgctacaac gtgatgggcg gcgggcgtcc ggttgtcgcg
    18301 ctgtgtctgc caactcgtta tctgcacgct aacagcggca tgatttcaaa agccgattac
    18361 gatgctctgc tcacgctgat acgggatttt ctgacgacct taactgcgga gaaagtcaac
    18421 gcgtttagcc agttccgtca ggtggattaa tgctaaacga acgccagtta aagattgtcg
    18481 atctgctgga gcaacagccg cgcacgcctg gagagctggc gcaacagact ggcgtttcag
    18541 gcaggaccat cctgcgtgat attgactatc tcaacttcac ccttaacggc aaagcccgca
    18601 ttttcgccag tggtagtgcg ggctatcagc tggaaatctt cgagcgccgc agcttttttc
    18661 agttgctgca aaagcacgat aacgacgatc ggctgctggc gctattatta ctaaatacct
    18721 ttactccccg tgcgcaactc gcctcggcgc ttaatttgcc agaaacgtgg gtagcagaac
    18781 gtctgccccg gttaaaacag cgttatgaac gcacttgttg cctggccagt cgccccggtt
    18841 tgggccattt cattgatgaa acagaagaga agcgcgttat cttgctggcg aacttgctac
    18901 gcaaagatcc gtttttaatt ccgctggcgg gcataacacg agacaacctt cagcatttat
    18961 ccacggcctg cgacaaccaa caccgctggc cgctgatgca gggtgattat ctctccagcc
    19021 tgattctggc gatttacgcc ctgcgtaatc agcttactga catatggccg caatatcccg
    19081 gtaacgagat aaaacaaatc gttgaacagt gcggcctgtt tctcggtgat aacgctgtaa
    19141 gaaccctgac gggtttgata gagaaacagc atcagcaagc gcaggtaatt tcagccgatc
    19201 atgtgctggg gttgctgcaa agggtgccag gcatcgcgtc attgaatatt attgatacgc
    19261 agttggttga gaatattacc gggcatttat tacgttgcct tgccgcaccg gtgtggattg
    19321 ctgagcaccg ccaaagcagc atgaataacc tgaaagccgc ctggcctgcg gcctttgata
    19381 tgagtctgca ctttattacg ctactgcgtg aacagctcga tattcccctt ttcgacagcg
    19441 atctgatcgg tttgtatttt gcctgtgcgc tggaacggca tcaaaacgaa cgccagccga
    19501 tcattttgct ctcggaccag aacgcgattg ccactattaa tcagctcgcc attgagcgcg
    19561 atgttttaaa ttgtcgggta attattgccc gtagcttaag cgaacttgtt gccattcgcg
    19621 aagagattga gccgttattg atcattaaca atagccatta tttactggaa gacgcggtaa
    19681 ataattacat caccgtaaaa aatatcatta cggctgccgg tatcgaacaa ataaaacatt
    19741 ttctggcgac ggcatttatt cgccagcaac cggagcgttt tttctctgcc cccggaagtt
    19801 ttcattattc gaatgtacgc ggtgaaagct ggcaacatat tacccggcaa atttgtgcgc
    19861 aattagtggc acaacaccat attaccgccg atgaagcaca acgcatcatc gcccgcgaag
    19921 gcgaaggtga aaacctgatt gttaatcgcc tcgccatccc acattgctgg agcgaacagg
    19981 agcgacgttt tcgtggattt tttattaccc tcgcccaccc gatcgaggtg aataacgaaa
    20041 tcattaacca tgtgttgatt gcctgcgccg ccgccgatgc gcgtcatgag ctaaaaatat
    20101 ttagctatct ggcaagcgta ttgtgtcagc atccggcaga ggttattgcc gggttaacag
    20161 gatatgaggc atttatggag ttacttcaca aggggtgaac tgtgtcggat gcgccgcatc
    20221 cgacactgca tttatcatca aaaacgttta acgcaggcta ttatagtcat caaccatttc
    20281 gttgtactcg cgtaatgctc tcgggaaaga gtcgtcgacc atccagcttg agtttgcgtc
    20341 ctgcagttgc tctttatccc agtcagagta cgggacttta tctctgatgc ggcgaacgta
    20401 ttttttagcc tcgagttgat actggcctgc cgaattaata aacgagaaat tcatgccgcc
    20461 tttatccgct tctttggcct gcgccactag cgtttccagt tcagacactt tcttcattgc
    20521 cgcatcaaca tcaaacttct cttgcgatat cagattatta atttgtttcg ctgaaatcat
    20581 gactgccaga gagtagtagt ggaatgtttt cccttctctt tcttcaatat ttttcagttc
    20641 cgcaagctgg cgcttatcgt taatttcctg aatcgccgca tgataagatt ctgctacagc
    20701 ttcaaaggct tccagatttt ttaagaatgt ctggtgcagc gttttccctt tcgcaaacgc
    20761 atcgtcttta taattttcct gggtgtaata tttatccatt tcgttaatgg tattacccaa
    20821 cgccacggca gcatcgatat aactcaccgc cacgccatca ataggttgca gcgcaggcgt
    20881 taacgccaca gcacttttta cgcctttttc acactcagcg aggttggatt cactaatgcc
    20941 gtagatgcca taaacagtac gctcttcacc ggtaggcccc tgtttaaaat ctttcagcca
    21001 gtcagcataa cgcgccagac tgctctgtac agggatctgt aacttgttat agcacttgat
    21061 atagacatcc attttttcgt cgaaaaccgc ttgttcatcg accaccggtt gcgcaggtgt
    21121 ttcaggttta gcttctgctt ttgcaacggg agcttcagga gctggtgcgg caggttgact
    21181 attggcaggc gggggcgttt ctgtttcggc ttttttatca tcacaacccg tcagaccgag
    21241 ggccatgatg gcgactataa ta
//
LOCUS       DAZFRN010000081        21052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFRN010000081 DAZFRN010000000
VERSION     DAZFRN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.081"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..137)
                     /locus_tag="ACHKUB_002891"
     CDS             complement(<1..137)
                     /locus_tag="ACHKUB_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO6655875.1"
                     /translation="MRYNGLNNMFFPLCLINDNHSVTSPSHTKKTKSDNYSKHHKNTL
                     I"
     gene            complement(315..1964)
                     /gene="actP"
                     /locus_tag="ACHKUB_002892"
     CDS             complement(315..1964)
                     /gene="actP"
                     /locus_tag="ACHKUB_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO6655876.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1961..2275)
                     /gene="yjcH"
                     /locus_tag="ACHKUB_002893"
     CDS             complement(1961..2275)
                     /gene="yjcH"
                     /locus_tag="ACHKUB_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO6655877.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2476..4434)
                     /gene="acs"
                     /locus_tag="ACHKUB_002894"
     CDS             complement(2476..4434)
                     /gene="acs"
                     /locus_tag="ACHKUB_002894"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO6655878.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4827..6263
                     /gene="nrfA"
                     /locus_tag="ACHKUB_002895"
     CDS             4827..6263
                     /gene="nrfA"
                     /locus_tag="ACHKUB_002895"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO6655879.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6308..6874
                     /gene="nrfB"
                     /locus_tag="ACHKUB_002896"
     CDS             6308..6874
                     /gene="nrfB"
                     /locus_tag="ACHKUB_002896"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO6655880.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6871..7542
                     /gene="nrfC"
                     /locus_tag="ACHKUB_002897"
     CDS             6871..7542
                     /gene="nrfC"
                     /locus_tag="ACHKUB_002897"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO6655881.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7539..8495
                     /gene="nrfD"
                     /locus_tag="ACHKUB_002898"
     CDS             7539..8495
                     /gene="nrfD"
                     /locus_tag="ACHKUB_002898"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO6655882.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGVAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8515..10233
                     /gene="nrfE"
                     /locus_tag="ACHKUB_002899"
     CDS             8515..10233
                     /gene="nrfE"
                     /locus_tag="ACHKUB_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO6655883.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFMRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10226..10609
                     /gene="nrfF"
                     /locus_tag="ACHKUB_002900"
     CDS             10226..10609
                     /gene="nrfF"
                     /locus_tag="ACHKUB_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO6655884.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10606..11202
                     /gene="nrfG"
                     /locus_tag="ACHKUB_002901"
     CDS             10606..11202
                     /gene="nrfG"
                     /locus_tag="ACHKUB_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO6655885.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11544..12857
                     /gene="gltP"
                     /locus_tag="ACHKUB_002902"
     CDS             11544..12857
                     /gene="gltP"
                     /locus_tag="ACHKUB_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO6655886.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12935..13624)
                     /gene="yjcO"
                     /locus_tag="ACHKUB_002903"
     CDS             complement(12935..13624)
                     /gene="yjcO"
                     /locus_tag="ACHKUB_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO6655887.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13718..15865)
                     /gene="fdhF"
                     /locus_tag="ACHKUB_002904"
     CDS             complement(13718..15865)
                     /gene="fdhF"
                     /locus_tag="ACHKUB_002904"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15446..15448),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO6655888.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16063..17529)
                     /gene="mdtP"
                     /locus_tag="ACHKUB_002905"
     CDS             complement(16063..17529)
                     /gene="mdtP"
                     /locus_tag="ACHKUB_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO6655889.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17526..19577)
                     /gene="mdtO"
                     /locus_tag="ACHKUB_002906"
     CDS             complement(17526..19577)
                     /gene="mdtO"
                     /locus_tag="ACHKUB_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO6655890.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19577..20608)
                     /gene="mdtN"
                     /locus_tag="ACHKUB_002907"
     CDS             complement(19577..20608)
                     /gene="mdtN"
                     /locus_tag="ACHKUB_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO6655891.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPFEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20627..20902)
                     /gene="ytcA"
                     /locus_tag="ACHKUB_002908"
     CDS             complement(20627..20902)
                     /gene="ytcA"
                     /locus_tag="ACHKUB_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO6655892.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4773 a         5545 c         5737 g         4997 t
ORIGIN      
        1 tcaattaacg tgtttttatg atgtttgctg taattatctg attttgtttt ctttgtatgt
       61 gatggacttg tgacagagtg gttatcgtta atcaggcaaa gagggaaaaa catattattt
      121 aaaccattat agcgcataaa atatccttat aattaacaag aaagaaaagg catattctct
      181 gcattatcat tttctacgac tgtcaaaaat cgctcatttt ttaatgagtt tatttgttta
      241 atattatggg aaaaggtgat gcatgttgga gaggaagagt attccccggt caggcgaccg
      301 gggaagggga gagattaatg tgcgcggcct tgctcaacgc caaagccggt ctgggagcgg
      361 ataaactgtg cacggaacag ttcacgctcg cgcgcacctt ccgctgagtt atcggttgcc
      421 gagaagaacc agatgccgag gaatgccacg gtgatagaga acagcgccgg gtattcatac
      481 gggaagatgg ctttttcgtg accaaggatc tgtacccaaa tcgtcgggcc gaggatcatc
      541 agtaccactg cggtaatcag ccccagccag ccacccatca tcgcgccacg agtggtcagt
      601 ttcgaccagt acatagaaag cagaatgatc gggaagttac agctcgctgc gatggcaaac
      661 gccaggccta ccatgaaggc gatgttctga ttctcaaaca gcacgccgag gataatggca
      721 atcacaccta aaatcagcac ggtgattttc gatacccgca gttcttcacg ttcggtcgcg
      781 ccttttttga agacgttagc gtacaggtca tgcgaaaccg ctgatgcgcc cgccagcgtc
      841 agaccagcaa ccaccgcaag aatggtggca aaagcaaccg ctgaaataaa accgaggaac
      901 aggttgccgc ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga
      961 tggcccgccg cgtctttata ttccggattc gcaccaacca gcatgatcgc gccgaagccg
     1021 ataataaagg tcagaatata gaagtagccc ataaatccgg tggcgtagaa cacgctctta
     1081 cgtgcttcgc gggcatcgct gactgtaaag aagcgcatca gaatgtgcgg caagcccgcc
     1141 gtaccaaaca tcagtcccag acccagagag agcgcggaga tcggatcttt caccagcccg
     1201 cccggcttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcact gaacagattg
     1261 ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc
     1321 aacagcacgg ctttgataat ctgtacccaa gtggtcgcca gcatgccgcc gaacaggacg
     1381 tacatcatca tcagcacgcc gaccagcacc accgcaatgt gatagttaag gccaaacagc
     1441 agctcgatca gtttacctgc gcccaccatc tgggcgataa ggtaaagcgc caccaccacc
     1501 agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacggta agaggccaca
     1561 tcggcaaagg tgtagcgccc caggttacgc agacgttcgg caatcaggaa caaaatgatc
     1621 ggccagccca ccaggaagcc cagcgagtaa attaagccgt catagccgga ggtaaacacc
     1681 agcgcggaga tccccaagaa tgaggcggcg gacatatagt ccccggcaat cgccagcccg
     1741 ttctggaagc cagtgatatt gccgcctgcg gtgtagtagt cgctacgaga acgtacgcgt
     1801 tttgatgccc agtaggtaat gccgagcgta aacacgacga aaatcaggaa cataataatc
     1861 gcctgccagt tcgttggctg gcgctctacg gccccgctaa tagcatccgc ggcgttagct
     1921 gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat
     1981 gcaggacttc attattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccgg
     2041 tgagaacaaa ggagatcaca atcactccaa caccaattgg aataccgcgt gtgacgctgg
     2101 tgttcggatt cagcggcgtg cccagccagc cgggcgcgaa ggcgatcagt aaaataaagc
     2161 cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga
     2221 ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatagtgcca ttcatcacag
     2281 attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa
     2341 cgctcgggta acccttgccg aatgtaggcc ggataaggcg ttatatgccg catccggcaa
     2401 tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta caaaccgtta ccgattcgca
     2461 tccggcaatt gtgggttacg atggcatcgc gatagcctgc ttctcttcaa gcagcttctc
     2521 gacaacgcca ggatcggcaa gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc
     2581 aattttgcgc agaatacggc gcataatttt gccggaacgg gttttaggca gggagtcggt
     2641 ccagtgcagc acgtctggcg tcgccagcgg gccaatctct ttacgcaccc agttgcggac
     2701 ttctgcgtac agttctggtg acggttcctc cccgtgatta agcgtgacgt aggcgtagat
     2761 cgcctgacct ttaatattgt gcggaatacc tactacggcg gcttcggcaa tcttcggatg
     2821 cgccaccagc gccgactcaa tctctgccgt ccccagacgg tgaccggaga cgttcagcac
     2881 gtcgtccaca cgcccggtga tccagtaata gccatcttca tcgcgacgcg cgccgtcgcc
     2941 gctgaaatac atatttttga aggtggagaa gtaggtctgt tcaaaacgtt cgtgatcgcc
     3001 aaacagcgta cgcgcctgac ccggccagga gtcggtgatc accaggctgc cttcggtagc
     3061 gccttccagc gggttacctt cgttatcgac cagcgccggt tgcacgccga agaacggacg
     3121 tgttgccgaa ccggctttca gctcggtagc gccaggcagc ggcgtgatca tgaaaccgcc
     3181 agtttcggtc tgccaccagg tatcgaccac cggacatttc tcgttgccga tttttttcca
     3241 gtaccactcc cacgcttccg ggttaattgg ctcgcccacg gaaccgagaa tgcgcagcga
     3301 cgaacggtcg gtaccctcga tcgctttatc gccttccgcc atcagcgcgc ggatcgccgt
     3361 gggtgcggta tagagaatat tgacctgatg cttgtccacc acctgcgcca tacgggcagg
     3421 cgtcggccag ttgggtacgc cttcaaacat cagcgtggtc gcaccgcagg ccagcgggcc
     3481 gtacagcaag taactgtgtc cggtcaccca gcccacatcg gcggtgcacc agtagatatc
     3541 acccggatga taatcaaaga catatttaaa ggtcagcgcc gcgtacacca gataaccgcc
     3601 ggtagtatgc agcacacctt ttggcttacc ggtagaaccg gaggtgtaga gaataaacag
     3661 cggatcttcg gcgttcatct cttccgcctg gtgctgatcg ctcgcttgct caaccaggtc
     3721 gtgccaccac aggtcgcgcc cttcctgcca gtcaattttc ccgccagtac gcttcagtac
     3781 caccacatgc tctacgctgg tgacgttcgg gtttttcagc gcgtcatcaa cgtttttctt
     3841 cagcggaata ctgcgcccgg cacgcacacc ttcgtcggaa gtgatcacca gtcgtgagtt
     3901 ggaatcaata atgcgcccgg caacggcttc cggcgagaag ccgccgaaaa tcaccgaatg
     3961 caccgcgcca atgcgggcgc aggccagcat cgcaaccgcg gcttccggca ccatcggcat
     4021 ataaatcgcc accacatcac cttttttaat gcccagctcg agcagggtat tggcgaagcg
     4081 gcagacgtcg cggtgcagct ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc
     4141 ttcccagatg atggcggtac gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc
     4201 cgccagattc agcgtgccgt cctcgtacca tttaatggac acattaccgg gggcaaagga
     4261 ggtgtttttc accttctggt aaggtttgat ccagtcaaga atttttccct gttcgcccca
     4321 gaaggtatca ggtacgttaa tagattgttg atacatcgcc tcgtactgct gagggtttat
     4381 caggcaacgg tctgcgatgt tggcaggaat ggtgtgtttg tgaatttggc tcatgctttt
     4441 gttctccttg taggatgtta ataatatgtg gcataagcgt taaatgtagg ggtattggca
     4501 gttttgttta gtatttgggc gacagatcac gcaaaagtag aattgtgcaa ataaacggca
     4561 gggtaatttt tgaaggtcag caacaaaagt tgattaattc tttgaggaac atgcagttat
     4621 gcatgctgtt gaaaaagagg aagatactga ctaactctaa agtggtattt tacatgcact
     4681 tacaattgat taaagacaac attcacagtg tggttatttg ttacacatag gggcgagcaa
     4741 tgtcatgaca gtgtaggtgc ggttactgtc gtgaaaaaca ataaaaaccg ccattgcaac
     4801 aatggcgcaa ttcggatgaa gcccctatga caaggataaa aataaacgca cgccgtatct
     4861 tcagcttatt gattcctttt ttctttttca cttctgttca cgctgaacaa acggcagctc
     4921 ccgcaaaacc tgtaactgtg gaagcgaaga atgaaacctt tgccccgcag catcccgatc
     4981 aatatctctc ctggaaagcc acctcggaac agtcagagcg tgttgacgcc ctggcggaag
     5041 atccacggct ggtgatcctg tgggcggggt atcccttctc gcgcgattac aacaagccgc
     5101 gtggacatgc ctttgctgtg accgatgtgc gtgaaaccct gcgtaccggt gcgccgaaaa
     5161 acgctgaaga tggtccgcta ccgatggcat gctggagttg taaaagcccg gatgtggcgc
     5221 gtctgatcca gaaagacggc gaagatggct acttccacgg taaatgggcg cgcggcggcc
     5281 cggaaatcgt caacaactta ggttgtgccg actgccataa caccgcctca ccagagttcg
     5341 ccaaaggcaa accggagtta accctttccc gtccgtatgc ggctcgcgcg atggaagcca
     5401 ttggtaaacc ttttgagaaa gccggacgtt tcgaccagca atcgatggtt tgcggtcagt
     5461 gccatgtgga gtattacttc gacggcaaaa acaaagcggt taaattcccg tgggatgacg
     5521 gcatgaaagt cgaaaatatg gagcagtatt acgacaaaat tgccttctct gactggacta
     5581 actccctgtc gaaaacgcca atgctgaaag cgcagcaccc ggaatatgaa acctggacag
     5641 cgggcattca cggtaaaaac aacgtgacct gtatcgactg ccatatgcca aaagtgcaga
     5701 acgccgaagg caaactctac accgaccata aaattggtaa tccgtttgat aacttcgccc
     5761 agacttgtgc gaactgccat acccaggaca aagctgcctt gcaaaaagtg gtcgcggaac
     5821 gtaagcagtc gattaacgac ctgaaaatca aggttgaaga tcaactggtt cacgctcact
     5881 tcgaagcgaa agcagcgctg gatgcaggcg cgacggaagc tgaaatgaag ccaattcagg
     5941 acgatatccg tcatgcccag tggcgctggg atctggcgat cgcttcccac ggcattcata
     6001 tgcacgcacc ggaagaaggt ttacggatgc tcggtacggc gatggataaa gcggcggatg
     6061 cacgcaccaa actggcgcgc ctgctggcga ccaaaggcat cacccatgaa atccagatcc
     6121 cggatatctc gaccaaagag aaagcccagc aggccattgg cctgaacatg gaacaaatca
     6181 aggccgagaa gcaggacttc atcaaaacgg tgatcccgca gtgggaagaa caggcacgta
     6241 aaaatggtct gttaagccaa taaccccgtt ccgcctcgca aggggcggaa aacacaatgg
     6301 agtgaatatg agcgtattac gttcgttatt aactgctggg gtgctggcgt cgggcctgtt
     6361 gtggagcctg aacggaatta ccgctacccc tgcggcgcag gcatctggtg atcgctacga
     6421 agttacccag cagcgtaacc cggatgccgc ctgtctggac tgtcataagc cagatatcga
     6481 aggtatgcat ggcaaacatg cttccgttat caacccgaat aacaaactgc cggtcacctg
     6541 caccaactgt cacggccagc catcaccgca acaccgcgaa ggggtgaaag atgtgatgcg
     6601 ctttaacgag ccgatgtaca aggtcggaga gcagaacagc gtctgtatgt cctgtcatct
     6661 gccggaacag ttgcaaaaag cgttctggcc gcacgatgtc cacgtaacca aagtggcgtg
     6721 cgccagctgc cattccctgc atccacagca agatacgatg cagacgttaa gtgacaaagg
     6781 acggattaag atttgcgtcg attgccacag cgatcagcgc accaatccga actttaaccc
     6841 ggcgtctgtt ccgttgctta aggagcagcc atgacctggt ctcgtcgcca gtttctcacc
     6901 ggcgtcggcg tgctggcagc cgtcagcggc accgcagggc gcgtggtggc gaaaacgttg
     6961 aatatcaatg gggtgcgtta cggcatggtg catgacgagt cgttatgcat cggctgtacc
     7021 gcctgtatgg atgcttgtcg ggaagtgaac aaggtgccgg aaggcgtctc gcgcctgacg
     7081 attattcgta gcgagccgca gggcgaattt cctgatgtga aatatcgctt cttccgtaag
     7141 tcttgccagc actgcgatca tgcgccgtgc gttgacgtct gcccgaccgg tgcgtctttt
     7201 cgtgatgccg ctagcgggat tgtcgatgtt aatcctgatc tctgcgtcgg ttgtcagtac
     7261 tgcatcgccg cctgtccgta ccgcgtgcgc tttatccatc cggtcacgaa gacggcggac
     7321 aaatgcgatt tctgccgtaa aaccaatttg caggccggta agctgcccgc gtgcgttgag
     7381 gcttgcccga cgaaggcgct gacgtttggc aatctggacg atcctaacag tgagatttcg
     7441 caactgctgc gccagaagcc tacttaccgc tacaagctgg cgctgggaac caaaccgaag
     7501 ctgtaccgcg taccgtttaa atacggggag gtgagccaat gacgcagact tccgcatttc
     7561 attttgaatc gctggtgtgg gactggccga ttgccatcta cctgtttttg attggtattt
     7621 ctgccggtct ggtgacgctg gccgtgctgt tacgtcgctt ctacccgcag gcgggcggtg
     7681 cagacagtac gttgctgcgc accacgctga ttgtcgggcc gggcgcggtg atcctcggtc
     7741 tgttgatcct cgtcttccac ctgacaagac cgtggacctt ctggaagctg atgttccact
     7801 acagttttac ctcggtgatg tcgatggggg tgatgctgtt tcagctctac atggtggtgc
     7861 tggtgctgtg gctggcgaaa atctttgaac atgatttgct tgccctgcaa caacgctggt
     7921 tgccgaagct ggggatcgtg caaaaggttc tgagcctgct gacgcccgtt catcgcggac
     7981 tggaaacgct gatgctggtg ttggcggtgc tgctgggggc ttataccggc tttctgctgt
     8041 cggcgctgaa atcgtatccg ttcctcaata acccgatcct gccggtgctg ttcctcttct
     8101 ccggcatctc gtccggtgtg gcggtagcgc tgatcgccat ggcgatacgc caacgcaaaa
     8161 atccgcattc cacggaagcg cactttgttc accgtatgga aatccctgtg gtatggggtg
     8221 aaatcttcct gctggtggcg ttttttgtcg gtctggccct gggcgatgac ggtaaagtgc
     8281 gtgcgctggt ggcggcatta ggtggcggtt tctggacgtg gtggttctgg cttggtgtcg
     8341 ccgggctggg gctgattgtg ccaatgctgc tcaaaccgtg ggtcaatcgc agttccggca
     8401 ttcctgccgt gctggcggcg tgtggggcca gtctggtcgg cgtgttgatg ctgcgctttt
     8461 tcattctcta cgccggacag ttgacggtgg cgtaagccag aaaagaggtg gtttctggac
     8521 gtattccttc ctgaagtcgg ttttctggcg ttgttgttaa gtctcggggt caacgtgttg
     8581 accccgttga cggccttcgc gggagtgcgg ttgcgctggc ctgccatgat gcgactcact
     8641 tgcatcggca ttctggcaca gttcgcgctc ctgctgctcg cctttggcgt actgacgtat
     8701 tgttttctca tcagcgattt ctcggtcatt tatgtcgcgc aacatagcta cagcctgctg
     8761 tcgtgggaac tcaagctggc agcggtgtgg ggcggtcatg aaggttcgct gctgctttgg
     8821 gtgctgctgc tttccgcctg gagcgcgctg tttgcctggc attatcggca gcaaaccgat
     8881 ccgctatttc cgctgacgct agccgtttta tctctcatgc tcgccgcact gctactgttt
     8941 gtggtgctgt ggtccgatcc cttcatgcgg atatttccac cagcaatcga aggccgcgat
     9001 ctcaatccga tgctgcaaca tcccggtctt atcttccatc cgccgctgct ttatctcggc
     9061 tatggcggtt tgatggtagc ggcgagcgtg gcgctggcga gtttactgcg cggcgagttt
     9121 gatggtgcct gcgccagaat ttgttggcgc tgggcactac ctggctggag cgcattaacg
     9181 gcggggatca tcctcggttc ctggtgggcc tattgcgaac tgggctgggg cggctggtgg
     9241 ttctgggatc cggtggaaaa cgcctcttta ttaccctggc tttctgccac tgcgctgctg
     9301 cacagtttgt ccctgacacg ccagcgggag attttccgcc actggtcgct gttgctggcg
     9361 atagtaactc tgatgctgtc gctactgggt accttaattg tccgttctgg cattctggtt
     9421 tcggttcatg cgttcgcact ggataacgtc cgcgccgtgc cgttgttcag cctgtttgca
     9481 ctgattagcc ttgcgtctct ggctctgtat ggctggcgag cgcgggacgg tggcccggtg
     9541 gtgcgttttt cggggttatc gcgggaaatg ttaatcctcg ctacgctgtt gctgttttgc
     9601 gcagtgctac tgatcgtgct ggtgggaacg ctttatccga tgatttacgg tctgctgggc
     9661 tggggacgcc tctccgttgg cgcgccgtat tttaaccgcg cgacgttacc gtttggcctg
     9721 ttgatgctgg tggtgattgt gctggcgacg tttgtctctg gcaaacgcgt gcagcttccg
     9781 gcgctggtag ctcatgcggg cgtgctgtta tttgccgcgg ggatcgtggt ttccagcgtc
     9841 agccgtcagg agatcagcct gaatttacag ccgggtcagc aggtgacgct ggcaggatac
     9901 accttccgtt ttgagcgcct cgatctgcaa gccaaaggca attacaccag cgaaaaagcg
     9961 atagtggcac tgtttgacca tcagcaacgc attggtgaac tgatgccgga gcggcgtttt
    10021 tacgaagcac gtcgtcagca aatgatggaa ccgtcaattc gctggaacgg catccatgac
    10081 tggtatgcgg tcatgggtga aaaaaccgga gcggatcgtt acgcttttcg cttgtatgta
    10141 caaagcggtg tgcgctggat ctggggggga ggattgttga tgattgcggg cgcattgtta
    10201 agcggatggc gggggaggaa gcgcgatgaa taaaggactt ctcacgctac tgctgttatt
    10261 tacctgtttt gcgcgcgctc aggtcgtaga cacctggcaa ttcgccaatc cgcaacaaca
    10321 gcaacaggcg ttaaatattg ccagccagtt acgttgtccg cagtgccaga atcaaaactt
    10381 actggaatcc aacgcgccgg tggcagtcag tatgcgccat caggtttaca gcatggtggc
    10441 ggaggggaag aacgaagtcg aaatcattgg ctggatgacc gaacgctacg gagattttgt
    10501 tcgctataac ccaccgttaa cgggtcagac gctggtgttg tgggcgctgc cagtggtgtt
    10561 gttactgctg atggcactga tcctctggcg agtgagggcg aagcgatgaa agagcccaaa
    10621 ataccggtga aaatgctgac aacactcacg attttgatgg tatttctctg tgtcggcagt
    10681 tatctgttaa gtccaaaatg gcaggcggta catgcggaat atcagcgtca gcgcgatccg
    10741 ctacatcagt ttgccagcca gcaaaccccg gaagcgcagc ttcaggcatt gcaggataaa
    10801 atccgcgcta atccacaaaa cagcgaacaa tgggcgttac tgggcgagta ctatctgtgg
    10861 caaaacgatt acagcaattc gctgctggcg taccgtcagg cgttgcaact acgtggtgag
    10921 aacgctgaac tgtatgcggc gctggcgacg gtgctttatt accaggccag ccagcatatg
    10981 accgcccaga ctcgcgcaat gatcgacaaa gccctcgcgc tggactctaa cgaaatcacc
    11041 gctctgatgc tgctggcttc cgatgcgttt atgcaggcga attacgcgca agccatcgaa
    11101 ctatggcaaa aagtgatgga tctcaactca ccccggatca accggacaca gctggtcgag
    11161 tcgattaata tggcgaaatt gttgcagcgg agatcagatt aatcatctgg tgatgaagat
    11221 tatgttattg agttgtataa aaaataatcg aatgaacata tgccaaaaat aatcaccaat
    11281 cagtattatt gcagattaaa caaataaaaa tctttccata acaaatggtt attcattaat
    11341 cctgccataa actcgcgtta ttatgcatta atgcagcgaa aagctctgtt gttaaagggt
    11401 tgcgcaacat accgcgcaaa tgatactgat cataagcgtt aaaaaaatct acaaaccaac
    11461 gcaacacaat tcatgccctg gcagtatgtc acgttctcgc gtttctgaac ggggaacggc
    11521 gctccattga ggaagttatt ctgatgaaaa gtataaaatt cagcctggcc tggcagattc
    11581 tgtttgctat ggtgctgggc attctcctgg gaagctacct gcactaccat agcgacagcc
    11641 gcgactggct ggtcgtcaat ttgctctctc cggcgggtga tatcttcatc catctgatca
    11701 aaatgattgt tgtgccgatt gtgatctcca cgctggtggt gggtatcgcg ggtgttggtg
    11761 atgccaaaca gctcgggcgt attggcgcga aaaccattat ttacttcgag gtgatcacca
    11821 ccgtcgccat cattttgggg atcactctgg cgaacgtctt ccagcccggt gccggggtgg
    11881 atatgtcgca gctggcgacc gtcgatatct cgaaatatca gagcactacg gaagcggtac
    11941 aaagcagttc ccacggcatt atgggcacga ttttgtcgct ggtgccgacg aacattgtgg
    12001 cgtcgatggc gaaaggcgaa atgctgccga tcatcttctt ctcggtgctg tttggtctgg
    12061 ggctttcctc cctgcccgcg acgcatcgtg aaccgctggt gaccgtgttc cgctccatct
    12121 ctgaaaccat gtttaaagtg actcacatgg tgatgcgtta tgcaccggtg ggtgtgtttg
    12181 cgctgattgc ggtgacggtg gctaactttg gtttctcttc tctgtggcca ctggcgaaac
    12241 tggtgctgct ggtgcatttc gccattctgt tcttcgcgct ggtagtgctg ggaattgtgg
    12301 cgcgcctgtg cgggttaagc gtctggatcc tgattcgtat tctgaaagat gagctgattc
    12361 tggcgtactc cactgccagc tctgaaagcg tactgccgcg aattattgag aagatggaag
    12421 cctacggagc accggcgtcg atcaccagct tcgtggtgcc gaccggttac tcttttaacc
    12481 ttgatggttc gacgctgtat caaagtattg ccgctatttt catcgctcag ctgtatggca
    12541 ttgacctgtc catctggcag gaaatcattc tggtgctgac gctgatggtg acctcgaaag
    12601 ggattgctgg cgtgccgggc gtgtcgtttg tggtgctgct ggcaacgctg ggtagcgtag
    12661 gtattccgct ggaaggcctg gcgtttattg ctggtgttga ccgtatcctc gacatggcgc
    12721 gtactgcgct gaacgtggtg ggtaatgcgc tggcggtgct ggtgattgcc aagtgggaac
    12781 acaaatttga ccgcaaaaag gcgctggctt atgagcgtga agtgctgggc aaatttgata
    12841 aaactgcgga tcaataattg aagattgccg gggttatcca cccggcaatg tgtggatgcc
    12901 tgatgcgacg ctggtgcgtc ttatcaggcc taccttaacc gttggttaat ttttcaaact
    12961 cttcacaccc ggtatcaaac ccttccatac agctcaggtt caaccagtgc agcgcctttt
    13021 gtttgttctt ctcgataaaa ccttcctcac cgtttaagaa catcattccc gcccagtact
    13081 cggaataacc ggttcgggaa attgcagagc tgcgtttgaa ataccaggtt gccttgtcat
    13141 cgtcggcctt aatgcccacg ccgttggcgt aaatcaaacc aagcagcatt tgggcatcga
    13201 cggcggagtc attctccaga tcttccgagg cgttttctaa cagcgaaatc gcctttggat
    13261 aatccggttt acccgcttga gtatttacca gaatatgcgc cagcgttact tcaccttctt
    13321 tactgcccgc ttgcgccgct ttctctgcaa gaacttttgc ttgtggatag tccagactga
    13381 ccggattggt gattttgatc tgcgccagca gcgcgcaggc atcagcatca ccgctgtcgg
    13441 cggctttctg tgcccaatat tcggctttgc tcaaatcgcc ggagctaaac cagctgtcgg
    13501 caagaaaata ttgtgcgcgt cggtccccgg cctctgctgc ctttaaatac tggctgccag
    13561 gctcggagtc gttcgcgtgg gcaaagaatg tcaaaaacaa catcaatgcg ataatttttt
    13621 tcattttgat aatacgattg ggtagggagt aaccagtata aagagttcga atggataaaa
    13681 aaacagcctc cgaaaggagg ctgtagaaag gactgtatta cgccagtgcc gcttcgcgca
    13741 ggcgagtttt caacttgttg tactcgtcaa tcacgtactg ctcggcggcg cgctgatcgg
    13801 cgatcggctc gacgcgcacg gcgcagtatt tgtattccgg cgttttcgta atcgggctta
    13861 agttttcggt aaccagctcg ttacaggcac caatccacca ctggtaggtc atgtaaatcg
    13921 cccctttgtt cggacgatcg ctgacctgcg cacgggtgat aattttgcct ttacgcgagt
    13981 gcacccaaac caatgcctca tcttcaatac ccagacgttt ggcgtcttcg gtattgattt
    14041 gtgcgtagcc aggttcatca gccagcgccg ccagtgccgc acagttaccg gtcatcgaac
    14101 ggcaagagta gtgaccaact tcacgcaccg ttgacagtac catcgggtac tcgtcggtga
    14161 gtttgtcgat tggcgctacc cagtcgcagg tgaagaactg tgccagaccg ttcggggtat
    14221 caaacttctc tttaaacaga taagaagtcc cctgatcggc atctgaagta tcgcggcaag
    14281 gccactgaat gaagcccagt tcgcccattt tctcgtaagt cgcaccgtag aaatccgggc
    14341 acagatgacg caactcatcc cagatctcct gggtgttgtt gtagtgcatc ggataaccca
    14401 tacgggtggc gatttcactg atgatttgcc agtccgtttt cagatcccat ttcggttcaa
    14461 ctgctttgaa gaaacgctgg aagccacggt cagccgcagt aaacacgcct tcatgctcgc
    14521 cccacgacgt tgacggcaaa atgacatccg ccgccgacgc ggttttggtc ataaagatgt
    14581 cctgaacgat aaccagttcc agatcttcaa aggctttacg tactgccgac agctccgcgt
    14641 cagtttgcag cggatcttcg cccataatgt acgcggcacg cacttcgcca tgcgctgcgc
    14701 ggtgcggcag ctcgctgatg cgataaccgg tatgagcagg caggctttcc acgccccagg
    14761 ctttggcgaa tttctcgcgg ttagccggat ctttcacgta ctggtatccc ggatacgtat
    14821 ccggcagcgc gcccatatcg caggcaccct gcacgttgtt ctgaccacga accgggttta
    14881 cacccgcatg cggcttaccg aggttaccgg tcagcatcgc gaggctggtc agagaacgca
    14941 cggtttccac gccctggtag aactgggtta cacccatgcc ccacaggatg gcggcgcttt
    15001 tcgcctgggc atacatccgt gccgcctgac gaatctcact ggcgctgacg ccggtgatat
    15061 cttcaaccga ctccggcgtg tagccttcaa cgattttacg atactcttca aagccttctg
    15121 tacgggaagc gacgaacgct ttgtcgtaca gattttcttc aataatgaca tggcccatcg
    15181 cattcaacag cgcgatgttc gagccgtttt tcagtgcaat gtgcatgtca gcaatgcgcg
    15241 cggtttcaat tttgcgcgga tcgcagacga taattttcgc cccgttacgt ttagcgttaa
    15301 ttacgtgatt cgccacgatt gggtgggaat ccgccgggtt gtacccgaaa acgaacacta
    15361 aatcggtatt atcaatttcg ttaatagcat tgctcattgc gccattaccg accgattggt
    15421 gcagacctgc aaccgatggg ccgtgtcaga cacgagcgca gcagtcaacg ttattggtac
    15481 caataacggc gcgcgcaaat ttttgcatta catagttggt ttcgttaccc gtaccacgcg
    15541 aggagccggt cgtctggatg gcatccggac cgtacttctc tttgatggcg ctcaggcgct
    15601 cggcaacgta attcagtgcc tcatcccagg aaacaggttc gagtttgccg ccacgctggc
    15661 gacggatcat gggggttttc aggcgcgggg tcaggatctg ggtatcgtta atgaaatccc
    15721 agccataata acccttcaga cacagggtac cctggttggt tttcccctgc gctgcctccg
    15781 cccggacgat tttgccgtta tcgacgacca ggttgatttt gcaacctgat gcgcaatagg
    15841 ggcaaaccgt gacgactttt ttcatcggtc tcgctccagt taatcaaatc acgcatacgc
    15901 gctctcgact acagtatgca tcttttatgc cacattttat ttggggtcat tccctgatat
    15961 tacgggcact atttattcaa aactctgacg aaaaacaggc tgtcgtcagt tttgacgtga
    16021 cgaaacgaaa taccgcgtga cagccatcac gcggcagaca ttttattttt tctcgacgac
    16081 gggagccgcc tgatacccgc cgcccagcga tttcatcaac tgaatgcttt ggatcacccg
    16141 gcggctgtcc agcatcagta atgacatctc ttcggcaagt actggcaacc gggcttcagt
    16201 ggcctgtaag cggctggtta agccgcgctg ataggcggcc tcggcagcgc gctgggtgaa
    16261 gcgcgttgct tccacgcgtt cagcctgcat ttctcgctcg tcgttgagcg tttgcagacg
    16321 cgtgccgttg acggcaacgt cacgcaccgc gttcagtact gactggttgt aacgttcaat
    16381 catcatgttg ctggcggcgc gcgtgccttc gagattggca ttcaaccgtc caccgtcaaa
    16441 cagcggcaat ttcagacccg ggatgaagtt gaactggcga ctggtttttt tgaataaggt
    16501 atccaggtgg atggcatcaa ggccgaaaaa cgctttgata tcaaagctcg ggtagaacag
    16561 cgcccgcgcg gaatccacct gatctaatga cgcctgaaca taccagcgca tggcttgcag
    16621 atccgggcgt ctggcgagca actcataaga gagtgtcgcc ggaatgccgg tctggactcg
    16681 tggtaatgcc accggtttga tctccggcat atcgctggcc cccgcgccaa tcaatgcacg
    16741 cagagattct cgcgtttcgg tgatttgccc tttgacggca gcaatttgtt tatcgaccgc
    16801 cagaatctgt gcccgcgcgc cgtggaaagg cacttgtgct tccagaccgt gcgccacttt
    16861 actctggtgc gctttcaccg cgtaatcaat cacatcgcga gtttgttcta acagatcgag
    16921 catctgatag ctggcctgca tactgtaata aagctgcgct acgcccgtgg tcagcgatag
    16981 ctctactgct gcggtttctg ccagcgcggc attatgcgcg ccaatggcgg cggcaaccgc
    17041 tgagcgatgc acaccccaca aatcaagatc cagtccggca aacaaaccta ctgtggcttc
    17101 cgtatagtac ggcccgtcca tacccagtgc gggcgcatcc atcgcataag ggcttaaaaa
    17161 gccgttcgcc gagacgcgtt ggcggttgag catccctaac gctgcgacct gtaattgtga
    17221 accggcatct aacaaatcgg cctgggactg cgctttttct tcccgcagtt tcgcttcggc
    17281 gagggtgtgt gaaccactta gcgtccgttg gatcagcgca tccagctgcg ggtcattgag
    17341 ttgtttccac cactgcgcct gcggccagcc ggagctggca agatgaatat cgtcggccag
    17401 tttgatttgt tccggtttga gctgttgatg aggtgcagaa tccttacgta ccagggcaca
    17461 gccggaaatc agcgtcgtgc tgccgagaat gctgcacaac agcagacgtg aaagttgacg
    17521 attgatcatt gcgtggctcc ttgtgcctgt tccgtggcgg gcgttaatgc cggggctgtc
    17581 cagtctggca agcgggcgaa aagctggcag acgtgttgct cctgttttaa taaggtgggc
    17641 aggatggcct gcgaggcggg tgtctcttca agcgttattt gaggagaacg gctgagtgcg
    17701 gttgccagac cggcagcgta tttctccagg gcgtcggcaa actgagctgc tctgtccggc
    17761 tgtagtgcgt tatccagcgc ctgcgccgag ttccaggtgg catcccaggc gtgaagaata
    17821 tcgcggccct gacgaataac cgtttgcgaa cgttcaatca gtaaggcgcg ttcttcgctg
    17881 tccagttgac gctccagcgc cacgcgttgg cacatctctt cacaggcatt aaacgccgca
    17941 tgcagaccga tacgaatttg cagataagtg cgcagagccg tgacttcctg ctggcgtggg
    18001 atccgcatta ctttacttaa catacccagc gtgccagcca gtttttgcgg cagtgtgcgc
    18061 gcttcacttt caggccagac aaaggtgtaa atcaccgcgg acaccacggt accaatgatg
    18121 atacccatgg cgcgatcgcg aatttccacc aggtcgtaca ccgggccaaa aacgttttcg
    18181 agcgtggcga gcgcgaaggt gaccaccatc tgtgtgccga tataagaaga gcgttcagag
    18241 ctggtggcaa tccatgcgcc caacaggaaa atcggtgcca gcacaaacag caattcgaca
    18301 atattgtcca gccagggcat gaccagtagc gtgaataaca gcgccagaat cgcgccgcaa
    18361 aaggccccgc caaaacgcag caccatcttc tggtacgacg aaccgacatt tgggttagcg
    18421 acgatcacgc atgtcagcat acaggtgtga atgccttccc agtccacgcc gctgtagaag
    18481 gtgtaacaga tcaaacaggc gagcagtgtt tttaccgcgt agcgcatata gtctggattg
    18541 gtaaaagcat cggcgaccat tgatggcggt ttggcggcgg gcgttggcgg cgtattcggg
    18601 tccatctggc ccagttgtaa caacgtctgg cagatattct ccaggttaca ttcccgtgcc
    18661 gccatcgctt cactttcagt gatccgccag tcgctttgcc agcactgacc ttcagcaacg
    18721 gcatgctgca gcttattgat ttctgaggct aatttttgcc gaaattcaat aattgcctga
    18781 gaatcagcaa aagaggtggg atcgtagcga ttcagcgtcg agtaaatgta ggttaccgtt
    18841 gccacgcagc tttgccacca tgcgctttga gttcgccagt tggcatcgtc cgcgagacaa
    18901 aagacattca gtttttgcag cgccagcgcc tctctttcaa tccgcgtttc gggtagcggt
    18961 gcgaggctgt ccgtcaggtg actaatggca tcatcaagcc gatcattaag cgcttgatgc
    19021 atttgcgtaa tggcacgact gggaaaccac agcacgccga ttaacgtcat cagcaaggtt
    19081 ggatagaggc caacaacgat acaccacagc gttaagcgca cgaccacttc cggatagtcg
    19141 agcatggcgg ggaaggtttg cccgtaaatg gcgacaatgg cgacagcgaa aaagaccagt
    19201 cccagccgat gggtgcgcat caaaaacata cagcccatca ggatcggccc ggcgatgatc
    19261 aatcggatca acggttcgcc gtatgaccat ttgtagatca aaaacaggct gccgatctcc
    19321 agcaccgtgg caaccacaaa caagatcgcg acaaatttgg tgtaaaacgc gttcgactga
    19381 ataccgtaaa acagcactgc cagcgataac gccacaaaag ggatctcaaa agtcatcgag
    19441 atcagaatca ccagcaggca gcctacccag agttgcacga tctgcggcac gcggcctggt
    19501 cgccgctcgc ttaactcttc atgaaagaac gccagcagcc tgaccaccgg taatggcagg
    19561 gagttgagcg cgctcattat tgcggctcaa gattagcgac tgccgaagcg ccgatgcgga
    19621 acatttccgg gtcaggttta tcgaccatga ttttgaccgg aaaacgctgg gcaacgcgga
    19681 cccagttaat agagcgagaa actttcggca ggccgcccag caccaggccg ccgtcatccg
    19741 gtagcacgcc gtagccaatc gaatccactt taccctcgaa ggttttgccg ctgtcactca
    19801 tcaggcgaat cgttgcgggt gtacctgagc gaatattttt cagatcggtt tcgcggaagt
    19861 tggcgatcac gtaccagtga cgagtgtcga ttagggtaaa aatagggcgc atggcagaag
    19921 caaattgccc gacggaggtt ttgagggaaa tgacccggcc atcaaacggc gcgcgaacgg
    19981 tcgccatttc cagatgaagt ttggtcaggg caatatccgc ttcgaccgcc gcacgttgcg
    20041 ccaccagcgc atcgacgccg ctgacggcgc tggcggctga ctgcgcctgt aacaatacgg
    20101 cattaagatc ggcttctgcg gcgcgctgcg cagttcttgc acggtcaacg tcttccgctg
    20161 agacaaaacc ctctttcagt aatggctcgg tgcggcgtaa tgtatctgtg gcctgtttcg
    20221 cggcggcacg ggctttttct accgtggcat taatcgagtc ggcaccaaac tgttgcgcat
    20281 caacgctacg ctgggtgagc ataatttgct tatccagcgc cgcgagggag gcttcagctt
    20341 tcgccagatt ggcttcgaac gggcgcgggt caatgcggaa cagcaaatcg ccctgtttga
    20401 ctgcctggtt gtcggtgacc gccagttcta caatgcggcc gctgacttcc ggcaccacat
    20461 caatggtatc tgctgacgcg taagcgtcat tagttgatgg cgcactgtct acgcgccaga
    20521 taacgaaaac aagggcaacc agcgccaatg caaccactaa cagagcaggg aatttactgc
    20581 gaggagcttt tttcggcgta ctttccatga gcgcgtctca aatagattag aaaaatgcca
    20641 gccagaacag catggcgtag agagcaaaaa gggcggtata aataattccg acaaatgcca
    20701 gattgatatt tgcccgctga ataactcgcc tcgtgatgag catcaaaata aggctggcaa
    20761 tggcgcagaa aaaccagctg ggataataag caccgatcac cgggattgcc ggagataatg
    20821 agcatcccga aaccatgaag acgggaatta tccaggctgt ttttttgagt tgcatcgcca
    20881 tgcgagagag aactgttggc atattaaata catcctgtct gctgcgaaca atatctgtag
    20941 tgaatgtcaa tatccattga aggcgtcagt atggctattt tctcgatgaa taaaaatagc
    21001 tttattcagc caccgcattc ataacttata tattttattt attaattggc tg
//
LOCUS       DAZFRN010000082        20988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFRN010000082 DAZFRN010000000
VERSION     DAZFRN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.082"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..296)
                     /locus_tag="ACHKUB_002909"
     CDS             complement(<1..296)
                     /locus_tag="ACHKUB_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655893.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFS"
     gene            complement(299..1066)
                     /locus_tag="ACHKUB_002910"
     CDS             complement(299..1066)
                     /locus_tag="ACHKUB_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655894.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(1069..1779)
                     /locus_tag="ACHKUB_002911"
     CDS             complement(1069..1779)
                     /locus_tag="ACHKUB_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655895.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1795..3303)
                     /locus_tag="ACHKUB_002912"
     CDS             complement(1795..3303)
                     /locus_tag="ACHKUB_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6655896.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GGWIIALHLADYQFHQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(3428..5875)
                     /gene="glgP"
                     /locus_tag="ACHKUB_002913"
     CDS             complement(3428..5875)
                     /gene="glgP"
                     /locus_tag="ACHKUB_002913"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO6655897.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(5894..7327)
                     /gene="glgA"
                     /locus_tag="ACHKUB_002914"
     CDS             complement(5894..7327)
                     /gene="glgA"
                     /locus_tag="ACHKUB_002914"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO6655898.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(7327..8622)
                     /gene="glgC"
                     /locus_tag="ACHKUB_002915"
     CDS             complement(7327..8622)
                     /gene="glgC"
                     /locus_tag="ACHKUB_002915"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO6655899.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(8640..10613)
                     /gene="glgX"
                     /locus_tag="ACHKUB_002916"
     CDS             complement(8640..10613)
                     /gene="glgX"
                     /locus_tag="ACHKUB_002916"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO6655900.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(10610..12796)
                     /gene="glgB"
                     /locus_tag="ACHKUB_002917"
     CDS             complement(10610..12796)
                     /gene="glgB"
                     /locus_tag="ACHKUB_002917"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO6655901.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(13069..14172)
                     /gene="asd"
                     /locus_tag="ACHKUB_002918"
     CDS             complement(13069..14172)
                     /gene="asd"
                     /locus_tag="ACHKUB_002918"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6655902.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            14364..14957
                     /gene="yhgN"
                     /locus_tag="ACHKUB_002919"
     CDS             14364..14957
                     /gene="yhgN"
                     /locus_tag="ACHKUB_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO6655903.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(15014..16354)
                     /gene="gntU"
                     /locus_tag="ACHKUB_002920"
     CDS             complement(15014..16354)
                     /gene="gntU"
                     /locus_tag="ACHKUB_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6655904.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(16358..16885)
                     /gene="gntK"
                     /locus_tag="ACHKUB_002921"
     CDS             complement(16358..16885)
                     /gene="gntK"
                     /locus_tag="ACHKUB_002921"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO6655905.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(17024..18019)
                     /gene="gntR"
                     /locus_tag="ACHKUB_002922"
     CDS             complement(17024..18019)
                     /gene="gntR"
                     /locus_tag="ACHKUB_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO6655906.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(18243..18938)
                     /gene="yhhW"
                     /locus_tag="ACHKUB_002923"
     CDS             complement(18243..18938)
                     /gene="yhhW"
                     /locus_tag="ACHKUB_002923"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO6655907.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(19061..20098)
                     /gene="yhhX"
                     /locus_tag="ACHKUB_002924"
     CDS             complement(19061..20098)
                     /gene="yhhX"
                     /locus_tag="ACHKUB_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6655908.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            20430..20918
                     /gene="yhhY"
                     /locus_tag="ACHKUB_002925"
     CDS             20430..20918
                     /gene="yhhY"
                     /locus_tag="ACHKUB_002925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6655909.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         5256 a         5620 c         5166 g         4946 t
ORIGIN      
        1 gaaaagacca gccagcaatg cagtctgttt tttaattcag gaggcgttct gcgggagaat
       61 tgccaaccga tgccagccat tacactgaaa aaaagtgcat attcaaccat taactcaagt
      121 tgtagttcct gaagagaaat acgcacaacc aggagcggaa aggagaatat tatttctgcc
      181 agaaaagtta acgtgaatat aagtacggct aagacaataa ccgtcggcca taccaataat
      241 ggcacgataa caaaaccgag aattacaggg ggaatataaa gccggaattt aatcattttt
      301 attttcctgg tgattcggat gatgcgtcat acatcgcgca taaacactca tcaattatcc
      361 agtcgtctac ccagacctga tgctgtgtgc cctgttctgg ctgctttggt gggttctcaa
      421 tcgcttgccg cgctctttct ttcagtaaag gatgattatt tctagcccag gcattgattt
      481 gggtggtaat ggcgttatcg atatcgtcct ggttatctct gatataatcc tcacaatatc
      541 tgttcaataa acaggcaaga gaaacggtcg accaaaagcc gggaactgaa ttaatggcat
      601 ccagcaatgg cgcaagagcg atatcggcac ggaaaaacat ttccagcata tactgtaatt
      661 cgacagcttc atcgccggat acccaatcag tcatatactg gcaggcaaat cgctgtaaaa
      721 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
      781 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
      841 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
      901 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
      961 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
     1021 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
     1081 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
     1141 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
     1201 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
     1261 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
     1321 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
     1381 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
     1441 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
     1501 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
     1561 ggacgttaag ccaatattta atgtgacatt atatcgaagc aatccctcaa gaccgtcagc
     1621 accgacgaac ttttcccctt catccagggc ctggttaatg agttgctgaa caatgccttc
     1681 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     1741 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     1801 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     1861 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     1921 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     1981 gttaatcgct agcagtttta tatttttatc gtcgttcatt tgtgttgcgt aataacggaa
     2041 ggtgtaacct attgcgttgt ttgtattacg gtattcggca acgactttaa tcattccctc
     2101 catcatactt gccacttctg tttcctgcgg cgagatcatg cggacatttt tcatgacctg
     2161 ggattgcatc accgtctggc taccagagtc ttccgggcgt tgccaggtct ggatctcctg
     2221 atcgttaccg ccaaccgtgc gccagttggt aattgcacca ctgaagatgt cacgcacttg
     2281 ttgttcggtc agggaattaa ccgggttatc tgcattgacg atgaaaacaa acgcttcacg
     2341 ggcaaaaggc gtgtaaatca aagtgacgcc cgattcctcc gcgcgttttt tctgcccacc
     2401 ggaaggttga gccacaaaga taatatcggc attaccttta acaatcttgt tatatgcttc
     2461 gggagtacga gagttcgcca gatattccca ttcgtgaaaa tcttccggca aaacgcttag
     2521 tgcataaaag gcagaggcat aaatggggta cgccgccgtt gcgccatcaa gtcgcggcca
     2581 gttttgcgtg aactgaattt gcggtttacc acgcagtggt gtcagttgat tattgagttt
     2641 atcgggtcgc caggcccagg tatcaatatt ttcacgcaca ccgacgccgg gattaagctg
     2701 tttatataaa taagcttggt aaccggcgac aaaaccaagc atgagaagga taaacaagag
     2761 agaattacgg caacgtaacc cggacgtgcc cgttaaagga tatctggtcg gccagccgta
     2821 ccccagcgaa aaactgatat gcgcgccaac aggtataatg actaaccacc agtactcacc
     2881 accgactaat gaggctaaca aaacgcccag ccaggggagc ataagatcag ccaaccaacc
     2941 cgctcccatc tggtgaaatt gataatctgc cagatgcagc gcgattatcc agccccccag
     3001 ggttaataat gcgggtacta aaaaaagaca ttgtcgccag acggctattt ctcttttttc
     3061 tggtgcacta tcgtccgccc acactcgccc cgagaaaaat agcattaatg cgcccagcgc
     3121 cagcataaaa atgttgagca gaaggtaaat tgttggcgtg ataaagatat tacaaacttc
     3181 aataataccg gcaagtccga cacccagcat ggcaataacc gccgccaaaa actgcgccag
     3241 aatggggatg ccgaaaaaag tcattaccag cgaggtcaaa atccattttc tgttttgcat
     3301 tattctttcc attctttctg aatggcgaaa ttatactccc cgagtcccct tgccccttct
     3361 ggacactttt ccgaaatgat ggcgaaaaaa acgggaccct ttggccccgt tctatttatt
     3421 agtgaaatta caatctcacc ggatcaatat gccagatatg atcggcgtac tctttgatag
     3481 tacggtcaga agagaagtag cccatattgg caatgttcag catcgctttt gcggtccact
     3541 cttcctgaag ctcgtagagt tcatcgactt tatcctgaca atcgacatag ctgcgataat
     3601 ccgccagtac ctggtagtga tcgccgaagt tgatcagcga atcaaccaga tcgcgatagc
     3661 gacccggatc ttccggactg aatacaccgc tgccgatttg cgtcagcacc tgatgcagct
     3721 cctcatcttt ctcgtagtat tcacgcggtt tgtagccctg acgacgcagt tcttccactt
     3781 cttccgctgt gttaccaaag ataaagatat tgtcagcacc gacatgatcc agcatctcga
     3841 cattcgcacc gtccaacgta ccgatagtca gcgcaccgtt aagcgcaaac ttcatgttac
     3901 tggtgccgga agcttccgtc cccgccagcg aaatctgttc agacagatcg gccgccggaa
     3961 tgatcaactg cgccaggcta acgctgtagt tcgggatgaa cacgactttc agcttgtcgc
     4021 caatctgcgg atcgttgttg atcactttcg ctacgtcatt gatcaaatga ataatgtgct
     4081 tcgccatgta ataggccgaa gccgccttac cgccaaaaat attcacgcgc ggtacccact
     4141 tcgcatccgg gtcggccttg atgcggttat agcgggtaat cacatgcaac acattcatca
     4201 attgacgttt gtattcgtga atacgtttga tttgtacatc gaacaacgcc tttggattca
     4261 ccaccacatt cagctgctgg gcgatatact ctgccagacg ctttttgttc tccagcttcg
     4321 cctgatgcac agcgtgatta accattggga aatcacagtg ttgttgcagc tcattaagca
     4381 ggctaaggtc ggtgcgccag ttacggccca ggtgttcgtc cagcacggct gaaagcgatg
     4441 ggttcgctac cgctagccag cgacgcggcg tcacaccgtt ggtgacgttg gtgaaacgac
     4501 ccgggaagat tttcgcaaag tcggcaaaca acgattgcac catcagatta gagtgcagtt
     4561 ccgatacacc gttaactttg tggctcacaa caaccgccag ccaggccata cgcacacgac
     4621 gaccgttgga ttcatcaatg atcgacgccc gtcccagcag atcggtatcg ttcggatact
     4681 gttcctgcaa ggttttcagg aaatagtcgt tgatttcaaa gatgatctgc aggtgacgcg
     4741 gcagaatttt acccagcata tcaaccggcc aggtttccag cgcctcgctc atcagcgtgt
     4801 ggttagtgta ggagaagacc tgacaacaca cctcaaacgc gtcgtcccag ctaaattggt
     4861 gctcatcgat cagcagacgc atcatctcag gaatcgacag taccggatgg gtatcattga
     4921 gatgaatcgc gattttatcc gccaggttat cgtaggtttt atgcaactga taatggcggc
     4981 ttaaaatgtc ctgaatggtc gaggaaacca ggaagtattc ctgacgcagg cgcagctcac
     5041 gcccggagta ggtggagtca tccggataca gtacgcgaga tacgttctcg gagtggtttt
     5101 tatcttccac tgccgcgaag tagtcaccct ggttgaattt accgaggtta atttcgctac
     5161 tggcttgggc actccacaaa cgcaacgtgt tggtcgcgtc ggtgtcgtaa ccagggatta
     5221 tctgatcgta agcgactccc agaatctctt cggtttcaat ccagcgcgtt tttttacctt
     5281 cctgctgaat gcgaccacca aaacggactt tatagcgcgt gttgtggcgt ttgaattccc
     5341 acgggttacc gtattccagc cagtagtctg gcgactcttt ctggctaccg ttaacgatgt
     5401 tctgcttgaa cataccgtag tcatagcgga tgccgtaacc gcgccccggc aaccctaacg
     5461 tcgccagaga atcaaggaag caagccgcca gacgtcccag gccaccgtta ccgaggcctg
     5521 ggtcattttc ttcatcaatc agctcttcga gatttaaccc catcgcttcc agtgcgccct
     5581 gtacatcttc gtaaattcct agcgacaaca tggcgttgga gagcgtacgg ccaatcaaaa
     5641 actccatcga caggtagtaa acctgacgag tttcttgcga caactgggca cggtttgaac
     5701 gtaaccagcg ctccacgaga cgatcgcgca cagcaaataa cgttgcgttc agccattcat
     5761 gtttattggc gacgaccggg tcctttccaa tcgtaaacat cagcttgtaa gcgatagagt
     5821 gcttaagagc ttctacgcta agcgtgggcg atgaatatgt aaacggagca ttcatatagg
     5881 cgtttcctga aaactatttc aagcgatagt aaagctcacg gtacgacttc gccgcgacct
     5941 gccagctaaa atccattgcc atagcctgac gttgcacaaa ccgccacagt gaaggacggg
     6001 accacagtac aaaagcacgt cgaatagccc gtaacagcga ccaggcatta ctatcttcaa
     6061 agacaaaccc actggcgacg ccatctgcaa ggttctcaag agaacagtca gaaaccgtat
     6121 cagcaagccc accggtgcgc cgcactaacg gcagcgtacc gtacttcaat ccataaagtt
     6181 gcgttaagcc gcacggttca aaacggctgg gcaccagaat gacgtccgcg ccgcccataa
     6241 tgcgatgcga aaatgcttcg tgatagccaa tctgaacgcc cacctggccg gggtattccg
     6301 ctgccgccgc aaggaaacct tcctgcagca ccggatcgcc cgcgccgagt agcgccagct
     6361 gcccgccctg ctccagaaga cccggtaagg cttccagcac caggtcgaga cctttctggc
     6421 tggtcagacg gctcaccact gcaaaaagcg gcactttatc gtcaacctta agccccattg
     6481 cgatttgtaa ctggcgctta ttttccgctt tatcttccaa cgtatcgcgg gtgtaacgcg
     6541 aggccaacag taagtccgtc tctggactcc agattttctc gtccacgccg ttcagtacgc
     6601 cggaaagacg cccttcacgg tgacgctgtt gcaacagacc ttccataccg taggcaaact
     6661 gcggttcggt gatctcgcga gcgtaggttg gactgaccgc cgtaatgtga tcggcatagt
     6721 acagaccggc cttcaggaaa gagatttgtc cgttgaattc cagcccatga atattaaaga
     6781 atgaccatgg caattggatg tcattcatgt gatgtgcata aaacatgcct tgataggcca
     6841 agttgtgcac agtaaacacc gacttcgccg gacgcccgcg cgccgccaga tacgcaggcg
     6901 caaggcctgc atgccagtcg tgcgcatgca ccacatcagg acgccagaat gggtcaagcc
     6961 cgctggccat ttctgcccca acccacccca gcagcgcaaa acgcaatacg ttgtcggtat
     7021 aggcaaataa gttggtatcg tgatacgggc ttcccggacg atcatagaga tgcggcgcgt
     7081 caatcaggta tatgccaacc ccgttgtaat gaccgaacaa cagcgtgata tgtccggcga
     7141 aggtatcacg acgggatact acctgcgcat cggtcacgcc acggcgaata tcgggaaatg
     7201 caggcaacag tacgcgagcg tcaacgccgt ctgcgatttg tgctgcgggt aatgccccaa
     7261 taacatcagc cagaccgccg gttttaagca gcgggaacat ctctgaacat acatgtaaaa
     7321 cctgcattat cgctcctgtt tatgccctaa cttccgtagc atttcgcgcg ttaccagcac
     7381 gatgccttct tctgaacgat agaaacgacg tgcatcttcc tctgcgtttt caccaatcac
     7441 catgccttcc ggaataacac aagcacgatc gatgacgcag cggcgcagac ggcacgagcg
     7501 acctacccat acttccggta acaatacggc ggaatcaatg ttgcagaatg aattcacgcg
     7561 aacgcgcgag aacagaacgg actgcaccac caccgaaccg gagatcacac aaccgccgga
     7621 taccagtgag ttaagggtca tcccgtggct accggagcga tcctgcacga atttcgctgg
     7681 cggtaatgat tcattgtagg tgcgaattgg ccaattgcga tcgtacatat ccagttccgg
     7741 caccacagag gccagatcga gattcgcttt ccagtaagct tccagcgtac ccacatcgcg
     7801 ccagtacggc tcggcatccg ggtcggattg tacgcaagag agcgggaacg ggtgcgcata
     7861 ggccagaccg gcttcggtga tcttgggaat caaatctttg ccaaagtcgt ggctggagtt
     7921 ctcatcacga tcgtcttctt ccagcagttc atacagatag tcggcgtcaa agacgtagat
     7981 acccatactc gccagagatt tgctcggatc gttcggcatt gacggtgggt tagcaggttt
     8041 ttcaacgaat tcgataattt tatcgttctc atcaaccgcc ataacgccaa atgcggaggc
     8101 ttcttcaatc ggtactggca tacaagcaac ggtgcaacgt gcgccttttt cgacgtgatc
     8161 gataagcata cgcgagtagt cttgcttgta gatatggtcg cccgccagga tcaccacgta
     8221 ttccgcttta taacggcgga taatgtcgag gttttgggtg accgcatctg cggtgccgcg
     8281 ataccagttt tcccctttca ttctctgctg tgctggcagc agatcgacaa actcgttcat
     8341 ttcttcattg aagaatgacc agccgcgctg aatgtgctgc accagagtgt gggactggta
     8401 ctgggtgatc acgcccatac gacggatccc ggagttgatg cagttagaca gcgcaaagtc
     8461 gataatgcgg aacttaccgc cgaagtgtac ggccggtttt gctcgcttat tggttaaatc
     8521 cttcaggcgg gtaccacgtc ctcccgccag tatcagggca acagatttca atggcagctg
     8581 gcgcgccaac attaagtgat cgttcttctc taaactaacc atgactaact ccttttttat
     8641 catctctgga acacacacaa tccgtgtgca ggtccctgcc agacagccgt aatcactggg
     8701 ttatcctctc cagcgaatgg gggaatggcg tgccactccc cagcaggtaa aacaatctct
     8761 gttacctcaa gcgtggcgtt aattgcgatc aaaaagcgat ccgagagcag aatttgcagc
     8821 tgtttcggcc cgttttgcca ctcatccgtg cttaaaggtt gagcatatcg atttagccaa
     8881 cggacattgc cgtcgccttc ttcccaccag cgattctcca ccaaagcggg aatgcgcttg
     8941 cgcagatgga ttaacgcggc ggtaaatgcg gttaaaccac tgcttgcctg cgaccagtcc
     9001 aaccaggtta attggttatc ctgacagtag gcattgttat tgccatgctg gctgtgacca
     9061 tgttcgtcac cggccagtaa catcggcgta ccctgggaga gcaacaacgt tgttaacagg
     9121 gcgtgaatgc tgtcgcgccg ccgttcaacc aggtcaagag aaccgcctaa cccttcttta
     9181 ccatgattgt tactgtaatt gttgttggtc ccgtcgcgat tttcttctcc gtttgcttca
     9241 ttgtgtttat ggttgaagca aacgcagtcg cgaagcgtaa aaccgtcatg cgcggtgacg
     9301 agattaatcg cggcactcgg cagacgacca ttacgtttaa aaacatcgct ggaggcagca
     9361 aaacgcccgg caaacgcccc cagaggcaaa tcataatgta gccagaaacg acgggcagca
     9421 tcgcggaaat gatcgttcca ctcggcaaac agcggcggga aatttcccac ctgataacca
     9481 ccaggagcga tatcccacgg ttcagcaatt aacttcacct gcgagagcac cgggcagttc
     9541 tggatagcgg taaacaacgg cgcatcctga cggaactctg gcgtacggcc catgactgcc
     9601 gccagatcaa agcggaaacc atcgacgtgg caggtttcta cccaataacg caggcaggcg
     9661 ctggcataat ccaccaccgc cggatgactc aaattgagcg tgttgccgca accggtccag
     9721 ttgtgataat cgccgtcttc tcttatccaa taatagctac ggttatcgat cccacgcagc
     9781 gagaataacg ggccgtcgag gtccagttcc gcactatggt tgagcacgat atcaagaatg
     9841 acttcgatac ccgctttatg cagtgctttg attgcatcgc gaaactcatc cagcgccgtt
     9901 tctggcgagc aggcatacgc cggatgcagc gcaaacatcg ccaccgggtt gtaaccccag
     9961 tagttactta gccccatgcg ttgcagacgt ggttcactgg caaactgcgc caccggcagc
    10021 agttccagcg cggtaatgcc caactgtttc aaatagttga tcatcaccgg atgcccgagg
    10081 gctttataag tgccacggat ctcgaccggg atctccgggt gcaagtacgt taatcctttg
    10141 acatgggctt cataaatgat ggtgctgccc cacggcgtgc gcggcggggc atcatcttcc
    10201 cagtcatagt gatcaaccac cactacgcat ttcggcgcaa tggcggcgtt gtcgcgatag
    10261 tcaggttcat tatgaccggc gtgcagcagc gggttatctt taaactcccc gtcaatttgc
    10321 cgcgcgcaag gatcaatcaa caacttcgcc gggttaaagc gatgcccctc ggcgggttgc
    10381 caggggccat gaacgcgata accataacgc aaacccgggc gcgcatccgg cagataaccg
    10441 tgccaaatgt cgccactgtg ccctggcaag tcatagcgat gttcctggcc attggcgtca
    10501 aagacacaca gttctacccg ctcggcatga gcggagaaaa gtgtgaagtt gacgccctga
    10561 ccgtcgtaat gcgcgccgag gggagcgggt ttgccaatgg cgagtcgtgt cattctgcct
    10621 cccgaaccag ccagatagtg gccagcggtg gtagtgtcag gcttagtgaa tgctgacgac
    10681 cgtggctggc aatctcatcg ctgtgtaccg tgccgccatt gcctgcatta ctgccgtgat
    10741 agtgcatgga atcggtattg aggatttcac gccatttgcc tggctggttt atgccgaagc
    10801 gataatcatg acgcggtacc ggcgtaaagt tactggcaac gatgatttcg ttaccctctt
    10861 tatcgcgacg cacaaagatc agcaccgagc gttctttgtc atccaccacc agccattcaa
    10921 agccgtacgg gtcaaaatcc agttcatgca ttgctttatg gtggcggtag gtgaggttca
    10981 gatcgcgcac cagacgctgg acaccgtggt gccagttatc gccgccttcc aacagatgcc
    11041 agtcgaggct ggcgtcatgg ttccactcgc ggccctgggc aaattcgtta cccatgaaca
    11101 gtagtttctt gcccgggaat gcccacatcc agccatagta ggcgcgcagg ttcgcgaatt
    11161 tctgccatgc gtcgcccggc atgcggtcga gaatcgattt tttaccgtgg accacttcat
    11221 catgcgacaa cggcaggacg aagttttcag tgtagttgta gagaatcccg aaggtcagtt
    11281 tatcgtgatg atactgacga taaaccgggt cgagcttcat gtagtccagg gtgtcatgca
    11341 tccagccgag gttccacttg taccagaagc ccagaccgcc catatcctgc ggacgagaaa
    11401 cgccagggaa atcggtagac tcctcagcca ttgtcaccgc accggaaacc tgctcaccaa
    11461 gaatacggtt ggtattacgc aagaattcaa tcgcttcaag attctcgcgc ccgccaaatt
    11521 cgttcgggat ccactccccc tctttacggc tgtagtcgcg ataaatcatt gacgccaccg
    11581 catcgacgcg cagcgcatca ataccaaaac gttcaatcca gtaaagcgcg ttaccgacga
    11641 ggaagttact gacttcacgg cgaccatagt tgtagatcag cgtgttccag tcctgatgat
    11701 agccttcacg cggatcgctg tgttcataca agttcgtgcc atcaaattcg gcaagcgcaa
    11761 agtcatcagt cgggaagtgg cctggcaccc agtcgagaat cacgttcaga ccagctgcgt
    11821 gtgcggcatc aatgaaataa cggaagtcgt cgcgagtacc aaaacggcgg gttggtgcat
    11881 acaggccggt tggctgataa ccccaactgc catcgaaggg atgctcgtta atgggcagta
    11941 gttcgaggtg ggtaaagccc atccatttag cataaggcac cagttgatcg gccagctcgc
    12001 ggtagctcaa ccagaaattg ttgtcggtgt ggcgacgcca ggaacccagg tgaacttcat
    12061 aaatagagat tggcgcatca aactgattcg cttttttgcg ctcttcagtc tgtacaacct
    12121 tttccggcag cccgcaaata agagacgcgg tttccgggcg catttgcgct tcgaaggcat
    12181 aagggtcgga cttcagacgc aagttgccat tggcatcaat catctcgtat ttatagagct
    12241 gaccgttatg cgccccaggg ataaacagtt cccagatgcc gctctcttta cgcaggcgca
    12301 tcgggtgacg gcgaccgtcc cagtagttga attgcccaac caccgagacc cgacgggcgt
    12361 ttggagccca gacagagaaa cgcgtacctg tgacgccatc catagtatct gcatgcgcgc
    12421 ctaaggtttc atacgggcgc aggtgagtac cttcagataa tagccaggca tccatttcct
    12481 ggattagcgg accaaaacgg taaggatcat caatcaggtt ttgctgacca tgccagacaa
    12541 cagccaactg atagcggaaa aaattcttac gtcgcggaat gacgccgcta aagaatcccc
    12601 gtgagtcgag acactccagt tttgcgagtt tgcgcccggt tttcggttca atcacccaca
    12661 catcggtagc gtcgggtaaa agggcacgga cttccagtcc cgcggtggtt ttatgcattc
    12721 ccagtacgga aaaaggatcc gcaaaatggc ctgcaattag cgcgttaatc acgtctctat
    12781 cgatacgatc ggacatgctt gtcttcctgt tttattgtgt caccccatcc aattggattt
    12841 ttgacttctg gttgtgacat ttttttgacc tgaacggcgc agcactctgt gcatcctctc
    12901 tgcgtcgtcc tcacttcagg taaggctgtg aatactcatg tattcagcca cccttaaaga
    12961 atagccaatg ctctatttaa ctcccggtaa atcatgaaac atctgcgctt actcctgtat
    13021 tacgcactaa caggggcggc atcgcgcccc agatttaatg aataaagatt acgccagttg
    13081 acgaagcatc cgacgcagcg gctccgcggc cccccacagc agctggtcgc ccacggtaaa
    13141 ggctgacagg aactctggtc ccatattcag cttacgcagg cggcctaccg gcgtggtcag
    13201 cgtgccggta acggcagctg gggttagctc acgcatagtg atttcccgat cgttcggaac
    13261 gactttcgcc cacggattgt gcgcagccag cagttcttcc acggtcggaa tagacacatc
    13321 ttttttcaat ttaatagtga atgcctggct gtggcagcgc aatgccccga cacgcacaca
    13381 taaaccatct accggaatta cggaagatgt gttgaggatc ttgttggttt ccgcctgccc
    13441 tttccactct tcgcggctct gaccgttatc gagctgtttg tcgatccacg gaatcaggct
    13501 acccgccagc ggcacgccaa agttatccac cggcagctca ccgctacggg ttaaggttgt
    13561 gactttgcgt tcgatatcga gaatagcaga ggacggggtc gcgagttcat ctgccacatg
    13621 gccatacaga tggcccatct gggttaataa ctcacgcata tgtcgcgcac caccgccgga
    13681 agcggcctgg taggttgcaa cggacaccca atcaacaaga tcattggcga ataaaccacc
    13741 caacgacatc aacatcaggc ttacggtaca gttaccgcca acaaaagtcc tgatgccatt
    13801 atttaatccg tcggtaatga cgtcctgatt gacggggtca agaatgatga tggcgtcatc
    13861 tttcatgcgc agagacgatg ctgcgtcaat ccagtaacct tgccatccgc tttcacgaag
    13921 ctttggatag atttcgttgg tataatcgcc gccctgacag gtcacaatga tatcgagggc
    13981 ctttagcgcc tccagatcaa aggcatcctg aagtgtgcca gtggttccgc caaaagacgg
    14041 cgcagcctgg ccaagctgag aagtagaaaa gaagacaggg cgaatggcgt cgaagtcgcg
    14101 ctcttcaacc atgcgttgca tgagaacgga gccgaccata ccgcgccagc cgataaaacc
    14161 aacatttttc ataagcgttt tttcctgcaa agatgtgtgc tgtataaatg tgccggtctc
    14221 ctcttggcac atctttcacc atacaaaaag cagccaaagt cgcaagtgaa attaatcaat
    14281 gatagcgaag ccatcagtaa tgcgacttat cctgctttgt tagcacgcag aaagtccgcg
    14341 gcaattatca gggaatttga gttatgaatg aaatcatttc tgcagcagtt ttattgatcc
    14401 tgattatgga tccgctcgga aacctaccta ttttcatgtc cgtactgaaa catactgaac
    14461 cgaaaagacg gcgggcaatc atggtgcgag agttgcttat tgctctcctg gtgatgctgg
    14521 tgttcctgtt tgcgggtgag aaaattctgg catttctcag cctgcgagca gaaaccgtct
    14581 ccatttctgg cggcatcatt ctgtttctaa tcgccattaa gatgattttc cccagcgctt
    14641 caggaaatag cagcgggctt ccggcaggtg aagagccatt tatcgtgccg ttggcaattc
    14701 cgttagtcgc cgggccgact attctcgcca cgctgatgtt gttgtctcat cagtacccga
    14761 atcagatggg gcatctggtg attgctctgc tgctggcctg gggcggcacc tttgtcatcc
    14821 tgctacagtc ttcgctattt ttacgtctgc tgggcgagaa aggggtgaac gcacttgaac
    14881 gcctgatggg attgattctg gtgatgatgg caacccagat gttcctcgac ggcattcgaa
    14941 tgtggatgaa ggggtaacga ggagagtttt tcgaaaagaa cccggtagcg tcacaactac
    15001 cgggcgaaca aacttaactc aacagctgga acgcaatcat cccaacgatg gcaccgacag
    15061 tgccgaggat ggtttccatc atggtccagg ttttcagcgt ttcggcttcg gtcgcgccgg
    15121 taaatttacc gaacaaccag aaaccggcgt cgttaacgtg gctgacaaca atcgaaccac
    15181 cagcgataca aatcgacagc gccgccattt gcgcaccgga gtagttcagt tgttcaataa
    15241 ccggcatcac cagtcctacc gccgttaaac aggctacggt ggcagaaccc tgaatgatgc
    15301 gcactgcagc ggccagcacg aagcaggtga tggctatcgg caggcccatg ccagttaacg
    15361 cttcgcccag tgccggacct acgccagagt caaccagcac ctgtttgaac acgccacctg
    15421 caccaatcac cagcagaatg atccccgccg gttgcagcgc gtgaccgcaa atctccatca
    15481 ctttgtcttt tggcatgccc tgacgcattg ccaggccata aatcgctacc agacaagcaa
    15541 ccagaatcgc ggtaaacgga tgaccaataa actcgaacca ttcgtaagcg gtagagcctt
    15601 ctggcacaaa acgcgcggca atggttttca gccccaccag caccagcggc aacaggatca
    15661 gcgacaggct gaatccgaaa gatggcatct tgccttcgcc gagatgcggt tcgctgatgt
    15721 cgtcaggaat atgcaactcc acgtagcggc tgatgaaatt accccacagc ggcccggcaa
    15781 taatcattcc cggaattgcc gcacacaggc caatcaggat catccagcca aaatcggcgt
    15841 tcatctgcga tgccagcagc attggcgctg gccctggcac caggaacgcc gccgctgccg
    15901 ccacgcctgc gaataatggg attaccagct tcaccaggtt cgtaccggtg tggcgcgcca
    15961 ttgagaaagc aacgctaatc agcagaacaa tcgccacttc aaagaacagc ggtagcgcac
    16021 agaccagccc cgcaaggccg atggcataat gcgcgcggct gtgaccgaag gatttaagca
    16081 ttttgacggc aatctgatcg actgcgccgg tttcatgtaa gatcttgcca aacatagctc
    16141 ccagggcgac aaccaccgcc aggaagccga gggtgcctcc catccctttt tccatcgtcg
    16201 ctgcgatttt atcgagcggc ataccggaaa aaaggccagc ccccatggac accaccatca
    16261 aagccaggaa agcgtgcata cgcgccttca tgactaaaaa cagcagcagt aaaacagacc
    16321 ctactgctgt taaaacaagc gttaatgtag tcactactta tttgcctttt ttaataacct
    16381 caatggtgct tgccacaaca ccttccagcg gttgatcgat atccaccacc agtacatcag
    16441 tttcgtccgc acccggctcc tgcagcgttt caaactgcgt caccaacatt tgggttttaa
    16501 agaaatggcc tttgcgcgct ttcaggcggc tttcaatcac gtcaaaatcg cctttcaggt
    16561 agatgaaaga gagattcgga ttaccttcac gcagcaagtc gcgatagtgt tttttcaatg
    16621 cagaacagac gatcagtgac actttattag tgcgctgcat agcaaacgcg gcgtcgttca
    16681 gcgcctgcaa ccacggtttg cgatcgtcgt cattcagcgg ttcgccagac gccatttttt
    16741 cgatattgcg ccgtggatgg aggaaatcgc catcaagaaa cgcggcatga agttgatgcg
    16801 ccacttcact ggcgaccgca gatttgccgc tgcccgatac gcccatcaag acgtaaatgt
    16861 ggtgatcatg gttagtcgtg ctcaaagtgg tgcccccaca atacaagaat taaaaatgtt
    16921 acgggtaact gttatcggta acattgtcca gccggacaat atcagaagca atatccatcc
    16981 gtgccttaag tgtataagtg tgagctactt caaatttgta ggcttaaata gatccgcccg
    17041 gtgacaaggt gaaacctaaa tctaacattt tcggtgtcac agattcgcca cgaatacgcg
    17101 ccagcaggcg ttcagcgcca atactgccca tccgctcacg cggcgtcagc acgctcgcta
    17161 accgcggctc catcacctgg ccaatgtcat gaccgtggaa accggcaatc gccatatcgt
    17221 caggaacctt caatcccaga cgctgacatt caaacgccgc gccgaccgcc aggtcatcgt
    17281 tggtacagaa cacgccatcc agctgcggat attcccgccg cgcctggcga atcagttcaa
    17341 taccggaaga gtaagaagaa gattgctcaa ccatcacgct atacggcacc agacctgcat
    17401 ccagcatcgc ctgttcgtat cccttctgtt tgatgatagt acgttcgtcg agacgcgcgc
    17461 cgagataggc aatgtggcaa tgcccgcgag caataatggc ggtggtcatc tggcgtgccg
    17521 cttcaaagtt atcaaaaccg acggcgatat cgaggcacgg cgactggctg tccatgagtt
    17581 ccaccaccgg gatccccgcc acttcaatca tctttaaggt gcgcggcgtg tgggtacgtt
    17641 cggtgaggat cagtccgtcg atattccatg agagcatgga ttcgagacgt tcttgctcca
    17701 tttccggttt ataaccgtag tgcgccagca tggtctgata accgtgcgcg tcggtgacgc
    17761 tttcgattcc gcgtaatact tccgcgaaaa cctggttggt gagagaaggt aacaggacgc
    17821 caatcgcccg gctggtggcg ttagagagga tatcgggcgc acgattggga atatagccca
    17881 gttcatcaag agcggcggca atcttgccgc gtagagcgac ggaaacctgc tccgggttgc
    17941 gtaaaaaacg gctgaccgtc attttggtca cgcctacacg gtcagccaca tcctgaagta
    18001 cgggtctttt ctttttcatc gtcctgaagg tacaaaagag atagattctc ttagtttaac
    18061 acggacgcat cagaaccttc ccggggaaaa cagtgcttgt ttacaattat tagatataca
    18121 ctaaatcaat tgcatcgcat tgtgctaacg acgcggcaaa accccggaag cgtgcatcag
    18181 cacgcgaccg gagcgagcga ggaagcctgc aaaaaggcag gcttcaaaga tgccgttgag
    18241 ttttaaaccg gcggcagatc gaacagtaac acttcgctgt cgctatccgc atggatggag
    18301 attgcctgct catcccagat tgccagacca tcgctggtcg aggctttcac gccgttaatg
    18361 gtgacattgc ctttcaccac ctggatccag acgcggcgtt cagcggcaat ctgatgcacc
    18421 gactgctcat ctttcagcaa cgcccaacga tacagttcca tatcctgatg cactttcagc
    18481 gaaccatctc gcgcatccgg cgagagcacc agctgtttgc cctgcacggc atcgaagcga
    18541 cgctgttcat aacgcggcgt aatgccattt tcttcaggca tgatccagat ctgatacaga
    18601 tgcagacgct cggtgctgtt tgggttgtac tctgagtgac gaatacccgt acccgcactc
    18661 ataatctgga actcacccgc cggaacctgc tctttattgc ccatgctgtc ctgatgctca
    18721 acagtacctt ccagcacgta ggtcaaaatt tccatatctt tatgcgggtg agtgccgaag
    18781 ccctgccctg cttcaatcac gtcgtcgtta atcacgcgca gcgcggagaa gcccataaag
    18841 ttcggatcgt aatagttggc aaaagagaaa gtatgccagg agtccagcca gccatgattt
    18901 gcatgaccac gttcatttgc tttgcggaag tagatcattt gtattcgccc cctgaatgat
    18961 ttcgatgggc ttagtgtgga cccgatctgc ctgggatgat agagggtgaa aattgacccc
    19021 tctgttcaaa aaaattgaac tattcgaggg gccatcaaac ttacttagcg agagttactg
    19081 tggagggaga ggcttgctca aatccgcgtt caaggatttc cagattggta agaacttcag
    19141 attccttgac gtaatttggc gcaccgtggg tgatggtttg atacaacgca tcataaacgc
    19201 gcccgtaatc gcccatctcc ggcttcatct cttctctgac cgtcacgccc tcgtcattga
    19261 catactccag cacaccgacc gaatcatccg ctgcgaatcc cggttcgccc ggcataatat
    19321 tagccttcag gctggtttcc tgctggtcga taccgtattt aataaacgaa cctttcttac
    19381 cgtgaacgat aaatttcgga taatcgattt tcaccagatg gctggttttg acgatggctt
    19441 tcaggtcgcc ataaaacagt tgcgcttcaa aggtgtcgtc aggattggct ttattacgca
    19501 ggctgcggat gtcataagcg acgtgatccg ggcgaccgaa cagagaaata atctggtcca
    19561 tcgtatgcac accaaggcca taaaacgcgc catcctgcgg cagcccaggt ttggtctctg
    19621 ccaccgggcg gtaatagtca aaatggcttt ccacttcaac aatctctccc aacttgccac
    19681 tttcaatcgc ttttttcgct gtcaggaagc aggagtcaaa gcgacgattc tgatacggcg
    19741 tgacggtcag ccctttgctt ttcgccaacg caaacagctc cttcgcctgg gcaagcgtcg
    19801 gggtgaacgg tttttcgacc agcacatttt tcccggcttc cagcgcgcgt ttcgcgtact
    19861 cgaaatggct gtccgcgtgg gtgcagacaa caaccagctt aacatcggga tcgtttagta
    19921 cttcgtcgag atcgctggtg aaatggatat gggaataaat gggagcctgt tcttccggct
    19981 tcgcatggcg acgaaaaata tgcgcgacat gccagctatc cttgcggtta agtacatacg
    20041 gcagatggta acgggtggtg cttttgccga agccaataaa ggcgcagttg atgaccatga
    20101 tttcgtcctt tttaaggtgg ttatttacac cttagcgcaa agcggacgtg gttcctactg
    20161 gagtgcgcat aacgaacaca agcactcccg tggataaatt gagaacgaaa gatcaaaaaa
    20221 aagccagcac ccggctggct aagtaatact ggaagcaatg tgagcaatgt cgtgctttca
    20281 ggttctccgc gagggtcttc ctgatcgcga gacaataata atcattctca ttcgcacttg
    20341 tccaacactt tttgcaaaaa aatgcatttg actcgcattt gaaagtcaat gatgttgaaa
    20401 gggacattta ccccaaagag gacaaaggaa tgagtgagat agtaatacgc cacgcagaaa
    20461 cacgggatta cgaggccatc aggcagattc acgcccagcc ggaggtgtat tgcaacacac
    20521 tacaggtgcc tcatccttcc gatcatatgt ggcaggagcg actcgccgat cgtcccggca
    20581 tcaagcaact cgtcgcctgt attgatggag acgtcgtggg ccatctcacc attgacgtgc
    20641 aacagcgccc acgccgcagt catgttgccg attttggtat ctgtgtcgac tctcgttgga
    20701 agaaccgcgg cgtcgccagc gccctgatgc gagagatgat tgaaatgtgc gacaactggt
    20761 tgcgggtaga tcgcattgaa ctaaccgtgt ttgtcgataa cgccccggca attaaggtct
    20821 ataaaaaata cggctttgaa attgaaggga ctggtaagaa gtacgcattg cgtaatggtg
    20881 aatatgtcga tgcatattat atggcgcggg tgaagtaaga tagtgccctt tttctgagat
    20941 ggaaaaaggg tgtcattcaa aatcgacata ccttccttta aggtattt
//
LOCUS       DAZFRN010000083        20886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFRN010000083 DAZFRN010000000
VERSION     DAZFRN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.083"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            222..1604
                     /gene="dosC"
                     /locus_tag="ACHKUB_002926"
     CDS             222..1604
                     /gene="dosC"
                     /locus_tag="ACHKUB_002926"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO6655910.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1629..4028
                     /gene="dosP"
                     /locus_tag="ACHKUB_002927"
                     /gene_synonym="pdeO"
     CDS             1629..4028
                     /gene="dosP"
                     /locus_tag="ACHKUB_002927"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO6655911.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELLLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4286..4867
                     /gene="ddpX"
                     /locus_tag="ACHKUB_002928"
     CDS             4286..4867
                     /gene="ddpX"
                     /locus_tag="ACHKUB_002928"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO6655912.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            4881..6432
                     /locus_tag="ACHKUB_002929"
                     /pseudo
     CDS             4881..6432
                     /locus_tag="ACHKUB_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            6434..7456
                     /locus_tag="ACHKUB_002930"
     CDS             6434..7456
                     /locus_tag="ACHKUB_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6655913.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASNAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRLMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7453..8349
                     /gene="ddpC"
                     /locus_tag="ACHKUB_002931"
     CDS             7453..8349
                     /gene="ddpC"
                     /locus_tag="ACHKUB_002931"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO6655914.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8346..9332
                     /locus_tag="ACHKUB_002932"
     CDS             8346..9332
                     /locus_tag="ACHKUB_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6655915.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRSEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            9325..10251
                     /locus_tag="ACHKUB_002933"
     CDS             9325..10251
                     /locus_tag="ACHKUB_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO6655916.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(10307..10738)
                     /gene="osmC"
                     /locus_tag="ACHKUB_002934"
     CDS             complement(10307..10738)
                     /gene="osmC"
                     /locus_tag="ACHKUB_002934"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO6655917.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            11083..11298
                     /gene="bdm"
                     /locus_tag="ACHKUB_002935"
     CDS             11083..11298
                     /gene="bdm"
                     /locus_tag="ACHKUB_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO6655918.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            11400..11537
                     /gene="sra"
                     /locus_tag="ACHKUB_002936"
     CDS             11400..11537
                     /gene="sra"
                     /locus_tag="ACHKUB_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO6655919.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            11694..13391
                     /gene="maeA"
                     /locus_tag="ACHKUB_002937"
     CDS             11694..13391
                     /gene="maeA"
                     /locus_tag="ACHKUB_002937"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6655920.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            13525..14535
                     /gene="adhP"
                     /locus_tag="ACHKUB_002938"
     CDS             13525..14535
                     /gene="adhP"
                     /locus_tag="ACHKUB_002938"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO6655921.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            14682..14966
                     /locus_tag="ACHKUB_002939"
     CDS             14682..14966
                     /locus_tag="ACHKUB_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6655922.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(15017..15670)
                     /gene="fdnI"
                     /locus_tag="ACHKUB_002940"
     CDS             complement(15017..15670)
                     /gene="fdnI"
                     /locus_tag="ACHKUB_002940"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO6655923.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(15663..16547)
                     /gene="fdnH"
                     /locus_tag="ACHKUB_002941"
     CDS             complement(15663..16547)
                     /gene="fdnH"
                     /locus_tag="ACHKUB_002941"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO6655924.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(16560..19607)
                     /gene="fdnG"
                     /locus_tag="ACHKUB_002942"
     CDS             complement(16560..19607)
                     /gene="fdnG"
                     /locus_tag="ACHKUB_002942"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(19020..19022),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6655925.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            19839..20720
                     /gene="yddG"
                     /locus_tag="ACHKUB_002943"
     CDS             19839..20720
                     /gene="yddG"
                     /locus_tag="ACHKUB_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO6655926.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5101 a         5327 c         5302 g         5156 t
ORIGIN      
        1 atgtatgaat atgaaaaaac atacgatatt cgctgtcacc gaaaaataat tacgatgggc
       61 tatactcttc tgaagaacag tagcgtagat taaaactgtc ctaagtcgct tatttctttt
      121 cagtatatct tcatatttca ggagaatatg gtgcagttag tcgatctcgc acgttgcgtt
      181 ttgattccgt ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa
      241 gaatgaaaga tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag
      301 ccgcagaaat tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc
      361 gcatcgaccc gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa
      421 gtgcgatgga acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc
      481 taatacaaat ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa
      541 ttgtcgagat ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg
      601 attattctgc cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg
      661 ctatggaagt gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg
      721 aaaactatcg tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag
      781 cctcaatact ttcatgggaa atagatatta tctataaaat cctgctggat tctgatttag
      841 gcagcagttt gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac
      901 attattttag cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg
      961 gtattttcaa tcaaaccatg cgtaacacca ggaatttgaa taacagaagt ttgcgggtga
     1021 aatttctatt acagataaga aataccgtat cgcaaattat taccttgctg cgtgaattgt
     1081 ttgaagaagt atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc
     1141 gtttcctgcc gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc
     1201 tgtcagtgct gattattgac gttgataaat tcaaagagat caacgatacg tggggccata
     1261 acactggcga tgaaattctg cgtaaagtct ctcaggcctt ttatgacaac gtccgcagta
     1321 gtgattatgt tttccgctac gggggcgatg aatttatcat tgttttgact gaagcttctg
     1381 aaaacgaaac gttacgtacc gcagaacgta ttcgcagtcg ggtggagaaa accaaactga
     1441 aagccgcaaa cggcgaggat attgccctct cactttccat cggtgccgcc atgtttaatg
     1501 gtcatcctga ctatgagcgc ctcattcaaa tagccgatga agctctgtat atcgccaaaa
     1561 gacgaggtag aaaccgtgtt gaactctgga aagccagtct ttagatgcgc caggatgcag
     1621 aggtaatcat gaagctaacc gatgcggata atgccgccga tggcattttt ttccccgccc
     1681 ttgagcaaaa tatgatgggt gcggtgttaa ttaacgaaaa tgatgaagtg atgtttttca
     1741 accccgccgc agagaagctc tgggggtaca aacgtgaaga agtcattggc aataacattg
     1801 atatgctgat tccgcgggat ttgcgtcctg cgcatcctga atacattcgt cacaaccgtg
     1861 aaggcggtaa agcgcgcgtc gaggggatga gtcgggagct gctgctggag aaaaaagacg
     1921 gcagtaaaat ctggacccgt tttgcgctat cgaaagtgag cgccgagggg aaagtttatt
     1981 acctggcgct ggtacgggat gccagcgtag aaatggcgca aaaagaacag acccgacaat
     2041 tgattattgc cgttgaccat ctcgaccgac cggtgattgt cctcgatccg gaacgccata
     2101 ttgtgcagtg caatcgcgca tttacagaaa tgtttggtta ctgcattagc gaagccagcg
     2161 gtatgcagcc cgatacactc ctgaacattc ctgaattccc tgccgataac cgcattcgtt
     2221 tacaacagtt gctatggaaa accgcccgcg atcaggacga atttctgctg ttgacgcgca
     2281 ccggtgaaaa aatctggatt aaagcctcta tcagcccggt ttatgacgtg ctcgcgcatc
     2341 tgcagaacct ggtaatgacc ttctcggata tcaccgaaga acggcaaatc cgccagcttg
     2401 aaggcaatat tctcgccgcc atgtgcagca gcccgccatt tcatgaaatg ggggaaatca
     2461 tttgtcgtaa catcgaatct gttctcaacg aatcgcatgt ttcgctgttc gcactgcgca
     2521 acgggatgcc gatacactgg gcgtcatctt cgcacggtgc agaagttcaa aatgcgcaaa
     2581 gctggtcagc gaccattcgt cagcgtgatg gcgcgcctgc ggggatcctg caaattaaaa
     2641 cctcgtcagg agcagaaacc agcgccttta tcgaacgcgt ggcagatatc agtcagcata
     2701 tggccgcgct ggcgctggaa caggaaaaaa gccgccagca tattgaacaa ctcatccaat
     2761 ttgatccgat gaccggtctg ccaaatcgca ataacctgca caattacctc gatgacctgg
     2821 tcgacaaagc cgtctctccc gtggtgtatc tcatcggtgt tgaccatatt caggatgtga
     2881 ttgatagcct tggctatgcg tgggccgatc aggcattgct ggaagtggtc aatcgctttc
     2941 gtgaaaaact caaaccggat cagtatctct gtcgtatcga aggtacgcag tttgtcctcg
     3001 tgagcctgga aaacgacgtc agtaacatta cccagatcgc cgatgagcta cggaatgtgg
     3061 tcagcaagcc gataatgatt gacgataaac ccttcccgct taccttgagt attggcatca
     3121 gctacgacgt gggtaaaaac cgcgattacc tgctctccac tgctcacaat gcaatggatt
     3181 atattcgcaa gaatggcggt aacggctggc agttcttcag cccggcgatg aacgaaatgg
     3241 taaaagagcg tttggtttta ggcgcagcgc tgaaagaagc gattagcaat aaccaactga
     3301 aacttgttta ccagccgcaa atcttcgcag aaacgggtga actgtacggc atcgaagccc
     3361 ttgcgcgctg gcacgatccc cagcatggtc atgtgccccc ttcacggttt attcctctcg
     3421 cagaagagat tggtgaaatc gaaaatattg ggcgctgggt catcgcggaa gcttgccgtc
     3481 agttagcaga atggcgtagc cagaatattc atatcccggc gctttccgtt aacttgtcgg
     3541 cactgcactt tcgcagtaat caactgccta atcaggtgtc tgatgcaatg cacgcctggg
     3601 gtattgacgg ccaccagctg acggtagaaa tcacggaaag catgatgatg gaacacgata
     3661 ccgaaatctt taagcgcatt cagatcctgc gtgatatggg cgtgggctta tcggtagatg
     3721 attttggtac gggcttttcc ggattatccc gcttagtcag tcttccggta acggaaatca
     3781 aaattgacaa aagttttgtc gatcgttgtc tgaccgaaaa acgcatcctt gccttacttg
     3841 aagccattac cagcattggg caaagcctca atttaaccgt cgtggcggaa ggcgtcgaaa
     3901 ccaaagagca atttgagatg ctacgcaaga tccactgtcg cgttattcag ggatatttct
     3961 tttcccgccc cctacccgcc gaagaaattc caggctggat gagcagcgtg ttaccgctga
     4021 aaatctgaca aattcctctc gcccgcactc gcgggttttc atttaacgtg acactgtcac
     4081 ttaaatactg tgatttcagc tacgattccg ggagattcct tccttaactt cccctcttgc
     4141 accaaaactg cactacgctg cacaaatgtt gatcaaaagt taaaaaatat aactttaagc
     4201 cagcattctg gcgcttatcc cggcatggca tgagatctgc ataagcggaa agcgcagcaa
     4261 tttttgtctt atacaggaac cgtttatgtc ggataccacc gaactggttg atttagccgt
     4321 aatcttccct gatctggaga tcgaattgaa atacgcctgc gctgataaca tcacaggtaa
     4381 agctatttat cagcaagcgc gttgtctgct acacaaggat gcgattaccg cgctggcgaa
     4441 aagtatcagt atcgcccagc tgtcaggctt acaactggtg atttacgatg cgtatcgccc
     4501 acaacaagca caggcgatgt tgtggcaagc ctgcccagac ccgcaatatg ttgttgatgt
     4561 gacggtcggt tctaatcaca gccgtggcac ggcgatcgac ctgacgcttc gtgatgagca
     4621 cgggaacatc ctcgatatgg gcgcagggtt cgatgaaatg cacgagcgct cccatgccta
     4681 tcacccttcc gtcccgcccg ccgctcagcg caatcggctg ttgctgaatg cgataatgac
     4741 tggtggcggt tttgtcggta tctccagcga atggtggcac ttcgaattac cccaggcagc
     4801 gagttaccct ctgctcgctg atcaattctc ttgttttata tcccctggca cacagcacgt
     4861 cagttaatca ggaaactgtc atgaagagat cgatatcgtt tccgtcccac attgctcgcg
     4921 ctcgtccttg ccacaaattt cccggtttcg cacgccgccg taccgaaaga tatgctggtg
     4981 attggtaagg ccgccgatcc acaaaccctc gacccggcgg taacaataga taataacgac
     5041 tggacggtga cctacccgtc ttatcagcgg ctggttcagt acaaaacgga cggtgataaa
     5101 ggctcaaccg acgttgaagg cgatctggca agtagctgga aagcgtctga cgatcaaaaa
     5161 gagtggacgt tcaccctgaa agataatgct aaatttgccg atggcacacc tgtcactgcc
     5221 gaagcagtaa aactttcttt tgagcggcta ctaaaaatcg gccaggggcc agcagaagca
     5281 tttcccaaag atttaaagat tgatgctccc gacgaacata cggtgaagtt tacccttagc
     5341 caaccattcg caccgttcct ctacacgctg gcgaatgacg gtgcatccat tatcaatccg
     5401 gcggtcttaa aggaacatgc agcggatgat gctcgcggct tcctcgcgca aaataccgcc
     5461 ggttccggac catttatgct gaaaagctgg caaaaaggtc agcaattagt tctggtgcca
     5521 aatccgcatt accccggcaa taaaccgaac tttaagcgag tatcggtaaa aattatcggt
     5581 gaaagtgcct cccgtcgcct gcagctctcc cgtggcgaca ttgacattgc cgatgcgctg
     5641 ccggtggatc aactcaacgc cctgaagcag gaaaataaag tcaatgtggc agagtatccg
     5701 tcactgcgcg ttacctatct gtatctgaat aacagcaaag cgcctcttaa tcaggcggat
     5761 ctacgtcggg ccatttcctg gtctaccgat tatcagggca tggttaacgg cattctgagt
     5821 ggtaacggaa aacagatgcg cggcccgatt ccggaaggca tgtggggcta cgatgcgacg
     5881 gcaatgcaat acaaccatga cgaaacgaaa gccaaagccg aatgggataa agtgacgagc
     5941 aaacccacca gcctgacgtt tctctactcc gataacgatc cgaactggga gcctattgct
     6001 ttggcgacac aatccagtct caacaagctg ggcatcaatg tgaagctgga aaagctggcg
     6061 aacgccacca tgcgcgacag agtgggtaaa ggtgattacg acattgcgat tggcaactgg
     6121 agtccggatt ttgccgaccc gtatatgttt atgaattact ggtttgagtc agacaaaaaa
     6181 ggtctgccag gcaaccgctc gttctatgaa aacagtgagg tcgataagtt actgcgcaat
     6241 gcgcttgcga ccaccgacca gacgcagcgt acccgggact accagcaggc acagaaaatc
     6301 gtcattgatg acgctgctta tgtgtacctg ttccagaaaa actaccaact ggcgatgaac
     6361 aaagaggtga aaggctttgt gttcaatccc atgctggaac aggtcttcaa tatcaatacc
     6421 atgagtaaat aacgtgacct tctggagtat tttacgccaa cgctgctggg ggctggtgct
     6481 cgtggtggcg ggcgtctgcg tgattacgtt tattatctcg catctgatcc ccggcgatcc
     6541 ggcgcggttg ctggctggcg accgcgccag caatgccatc gtggaaaata ttcgccagca
     6601 attggggctc gaccagccgc tatacgtaca attttaccgc tacgtcagcg acctgtttca
     6661 tggtgacctg gggacatcca ttcgtaccgg gcgtccggtg ctggaagagc tgcgtatatt
     6721 cttcccggcg acgctggaac tggccttttg cgccctgctg ctggcgctac tgattggcat
     6781 cccactgggc atactctctg ccgtctggcg taatcgctgg ctggatcatc ttgtccgtct
     6841 gatggccata accggaatat ccacacctgc attctggctt gggctgggcg tcattgtgct
     6901 gttttatggt catctgcaaa tacttcccgg cggcggaagg cttgatgact ggctggatcc
     6961 accaacgcac gttaccggct tttatctgct cgatgcgttg cttgaaggca acggtgaagt
     7021 cttcttcaat gcgttgcaac atctcatctt accggcatta acgctggcgt ttgttcacct
     7081 ggggattgtc gcccgccaga tccgctcagc gatgctggaa caattgagtg aagactacat
     7141 tcgtaccgcc cgggccagcg gcttgcccgg ctggtatatc gttttatgtt atgcgctacc
     7201 caatgcgttg atcccatcga ttaccgtatt aggtctggcg ctgggcgatt tgttgtatgg
     7261 cgcagtgctc accgaaaccg tttttgcctg gcccggaatg ggggcatggg tagtgacatc
     7321 aatacaggcg ctcgacttcc ctgcggtgat gggatttgcc gtcgtggttt catttgctta
     7381 tgtgctggtc aacctggtgg tggatttgct ctatttgtgg attgatccgc gtatcggacg
     7441 tggaggtggt gaatgatgct aagcgaggaa acgtccgccg tacgcccaca aaaacaaacg
     7501 cgatttaacg gtgcaaaact ggtatggatg ctgaaaggca gtccgctcac cgtgaccggc
     7561 gcagtcatca ttgtattaat gctactgatg atgatttttt caccgtggct ggcgacgcat
     7621 gatcccaacg ccattgattt aaccgcccgc cttttgccgc cttctgcggc gcactggttt
     7681 ggcaccgatg aagtgggacg cgatctgttc agccgcgtac tggtcggcag tcagcaatca
     7741 attctcgccg gattagtcgt ggttgccatt gcgggtatga ttggttcgct actcggatgt
     7801 ctatccggtg tgcttggcgg acgcgcagac gccatcatca tgcgcatcat ggacattatg
     7861 ctgtcgattc cttcgttggt actgacaatg gcactggctg ccgctctcgg gccgagttta
     7921 tttaacgcca tgctggcgat tgctattgtg cgaattccct tttatgtgcg cctggcgcgg
     7981 gggcaagcat tagttgtacg ccagtatact tatgttcagg cggcgaaaac ctttggcgcg
     8041 tctcgttggc atctgatcaa ctggcatatt ttacgtaact ccctgccgcc gctgatcgtg
     8101 caggcatcgc tggatatcgg tagcgcgatt ttaatggccg ccacgttggg atttattggc
     8161 ctgggtgcac aacaaccgag tgctgaatgg ggcgcgatgg tagcgaatgg tcgcaactat
     8221 gtactcgatc agtggtggta ttgcgcattt ccgggggcag cgattttgct taccgccgtc
     8281 gggtttaatc tctttggtga tggtattcgc gatctgcttg acccgaaagc aggaggaaag
     8341 cagtcatgac ccaacccgtt ctggacattc aacaactgca tttgagtttc cccggtttta
     8401 acggcgacgt tcacgcgctc aacaatgtgt ccttgcagat taaccgcagt gaaattgtcg
     8461 gtctggtggg agaatccggc tcaggtaaat cagtcaccgc aatgctgatt atgcgtctgc
     8521 taccgacggg cagttattgc gtacatcggg ggcagatttc actgctggga gaagatgttc
     8581 ttaacgctcg ggaaaaacag cttcgtcaat ggcgcggcgc acgagtggcg atgatctttc
     8641 aggaaccgat gaccgccctc aatccgacac gtcgaatagg tcttcagatg atggacgtaa
     8701 tccgccatca tcaaccaata agtcgtcggg aagccagagc taaagcgatt gccctgctgg
     8761 aagagatgca aatcccggat gccgtggaag ttatgtcgcg ctatccgttt gagctttcag
     8821 gtggtatgcg ccagcgggta atgattgcgc tggcattctc ctgcgagccg caattgatta
     8881 ttgccgacga accgactacg gcgctggacg tcacggtaca gttgcaggta ctgcgtctgc
     8941 ttaaacataa agcccgcgcc agtggaactg cggtactgtt catcagccat gatatggccg
     9001 tggtgtcgca actgtgcgat agcgtttacg tgatgtatgc cggaagcgtg attgagagcg
     9061 gcgtgacggc agacgttatc catcatcccc ggcatccgta taccattggt ttgctgcaat
     9121 gcgcaccaga acatggagta ccacgccagc tattacccgc cattccaggg acggtaccaa
     9181 acctcaccca tttgcctgac ggctgcgctt ttcgcgatcg ttgctatgcg gcaggtgcac
     9241 agtgtgaaaa cgtcccggcg ctgacagcgt gtggtgacaa caaccagcgc tgcgcctgtt
     9301 ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg cgacgtccat
     9361 atcaatttcc cggcccgtaa aaactggctt ggtaaaacta cggaacatgt tcatgccatt
     9421 aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg cgagtcaggc
     9481 tgcggcaaaa gcaccctcgc acagctttta atgggtatgc tgcaaccgag ccacgggcag
     9541 tacatccgtt ctggctcaca acgcattatg cagatggtgt ttcaggaccc gctctcttcg
     9601 cttaatccgc gcttaccggt gtggcgcatc atcacagaac cgctctggat agctaagcgt
     9661 agtagtgaac aacagcggcg agcgttggca gaggagttgg ctgtgcaggt gggtattcgt
     9721 ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca acgtatcgcc
     9781 attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc aacctctgcg
     9841 ctggatatct ccgttcaggc gcagatcctc aatttactgg taacgctaca ggaaaatcac
     9901 gggctgacct atgtgctgat ttcacacaat gtctcggtga tacgtcatat gagcgatcgg
     9961 gtggcggtga tgtatctcgg gcagattgta gaactgggcg acgcgcagca ggtgctgacg
    10021 gcacctgcac atccatacac ccgattattg ctggattccc tccccgccat tgataaaccg
    10081 ctggaggaag aatgggcatt acgtaaaacg gatctgccgg gaaaccgcac gttgccgcaa
    10141 ggctgttttt tctacgaacg ttgcccgctg gcaacccacg gatgtgaagt ccggcaatca
    10201 ttagcaataa gggaggacgg acgtgagctc cggtgctggc gggcgctgta gagatgctaa
    10261 caacgcatca ggcattcacg cgcctgacgc gtcatccggc aatgctttac gatttcaact
    10321 ggtaatccag cgtaatttcc gctttcagta cctgagagac cgggcatcct gcttttgctt
    10381 tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact tcactcttca
    10441 gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca tcggcggtgg
    10501 tatcaatcga tgttggcgta aatcccgctt cccccagcat taatgaaagc gccattgaga
    10561 aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa
    10621 aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc gctctcggtg gatactgttc
    10681 ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg attgtcattg
    10741 ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaaccg cacgaggata
    10801 agcaggatat tccgaataaa atacggcgaa aatattactt ctgcacttaa attaaattcc
    10861 cacaatagat aacctgaaat atatatctca agagagattg catcaatatt aatcaactca
    10921 tcacaataat aatacctatc aatatcatgg ggttaaaata taaaaatcat caaccaggac
    10981 taatcttaac aacgaagcgg caaatatttg ccatgctgaa tgtgctttat aaatctgcga
    11041 tccgtagcag acaccataaa tacacagaca cggagaatca ctatgtttac ttattatcag
    11101 gcagaaaatt caacagcaga acccgctctg gttaatgcca ttgagcaagg ccttcgggca
    11161 gagcacggtg ttgtcactga agatgacatt ctcatggagc tgaccaaatg ggtggaagct
    11221 tctgataacg acatcctcag tgatatctac cagcaaacga tcaattatgt ggtcagtggc
    11281 cagcacccta cgctttaagg tgctatgctt gttcggcaac ctaatttagg ggtttagcac
    11341 gtgtttcttc gctacggcga tgttgtcctt aaaactagca acaggattga ggagttaaaa
    11401 tgaaatcgaa ccgtcaggca cgtcatattc ttggactgga ccataaaatt tctaaccagc
    11461 gcaaaatagt taccgaaggt gacaaatcca gcgtggtaaa taatccaacc ggcagaaaac
    11521 gccccgctga aaagtaattc ataaccatca gtcctcaatg acgattaaac accattgcct
    11581 gcgcaatggt gtttttgttt ttatctgctc tatacttggg gccgacgccc tggcggtaaa
    11641 gcaaagacga taaaagcgtg ccagggatgg atattcaaaa aagagtgagt gacatggaac
    11701 caaaaacaaa aaaacagcgt tcgctttata tcccttacgc tggccctgta ctgctggaat
    11761 ttccgttgtt gaataaaggc agtgccttca gcatggaaga acgccgtaac ttcaacctgc
    11821 tggggttact gccggaagtg gtcgaaacca tcgaagaaca agcggaacga gcatggatcc
    11881 agtatcaggg attcaaaacc gaaatcgaca aacacatcta cctgcgtaac atccaggaca
    11941 ctaacgaaac cctcttctac cgtctggtaa acaatcatct tgatgagatg atgcctgtta
    12001 tttatacccc aaccgtcggc gcagcctgtg agcgtttttc tgagatctac cgccgttcac
    12061 gcggcgtgtt tatctcttac cagaaccggc acaatatgga cgatattctg caaaacgtgc
    12121 cgaaccataa tattaaagtg attgtggtga ctgacggtga acgtattcta gggcttggtg
    12181 accagggcat cggcgggatg ggcattccga tcggtaaact gtcgctctat accgcctgtg
    12241 gcggcatcag cccggcgtat acccttccgg tggtgctgga tgtcggaacg aacaaccaac
    12301 agctgcttaa cgatccgctg tatatgggct ggcgtaatcc gcgtatcact gacgacgaat
    12361 actatgaatt cgttgatgaa tttatccagg ctgtgaaaca acgctggcca gacgtgctgt
    12421 tgcagtttga agactttgct caaaaaaatg cgatgccgtt acttaaccgc tatcgcaatg
    12481 aaatttgttc ttttaacgat gacattcagg gcactgcggc ggtaacagtc ggcacactga
    12541 tcgcagcaag ccgcgcggca ggtggtcagt taagcgagaa aaaaatcgtc ttccttggcg
    12601 caggttcagc gggatgcggc attgccgaaa tgatcatctc ccagacccag cgtgaaggat
    12661 taagcgagga agcggcgcgg cagaaagtct ttatggtcga tcgctttggc ttgctgaccg
    12721 acaagatgcc gaacctgctg cctttccaga ccaaactggt gcagaagcgc gaaaacctca
    12781 gtgactggga taccgacagc gatgtgttgt cactgctgga tgtggtgcgc aatgtaaaac
    12841 cagatattct gattggcgtc tcaggacaga ccgggctgtt tacggaagag atcattcgtg
    12901 agatgcataa acactgtccg cgtccgatcg tgatgccgct gtctaacccg acgtcacgcg
    12961 tggaagccac accgcaggac attatcgcct ggaccgaagg taacgcgctg gtcgccactg
    13021 gcagcccgtt taatccagtg gtatggaaag ataaaatcta ccctatcgcc cagtgtaaca
    13081 acgcctttat tttcccgggc atcggcctgg gggttattgc ttccggcgcg tcacgtatca
    13141 ccgatgagat gctgatgtcg gcaagtgaaa cgcttgctca gtattcgccg ctggtcctga
    13201 acggcgaagg tctggtacta ccggaactga aagatattca gaaagtctcc cgcgcaattg
    13261 cgtttgcggt tggcaaaatg gcgcagcagc aaggcgtggc ggtgaaaacg tctgccgaag
    13321 ctctgcaaca ggccattgat gataatttct ggcaagccga ataccgcgac taccgccgta
    13381 cctccatcta agcctgcgcc cggtagtgaa ggctaccggg ctatttctct ctcccttttt
    13441 cagatctcat ccatactggg tagtggcgaa taaatctcat ttgcctcacc tgctatgcag
    13501 aacatcatcc gaaaaggagg aactatgaag gctgcagttg ttacgaagga tcatcacgtt
    13561 gacgttacgg ataaaacact gcgctcactg aaacatggcg aagccctgct gaaaatggag
    13621 tgttgtggtg tatgtcatac cgatcttcat gttaagaatg gcgattttgg tgacaaaact
    13681 ggcgtaattc tgggccatga aggcatcggt gtggtggcag aagttggccc aggtgtcacc
    13741 tcattaaaac caggcgatcg tgccagcgtg gcgtggttct acgaaggatg cggtcattgc
    13801 gaatactgta acagtggtaa cgaaacgctc tgccgttcag ttaaaaatgc cggatacagc
    13861 gttgatggcg ggatggcgga agagtgcatc gtggtcgccg attacgcggt aaaagtgcca
    13921 gatggtctgg actcggcggc ggccagcagc attacctgtg cgggggtcac cacctacaaa
    13981 gccgttaaac tgtcaaaaat tcgtccaggg cagtggattg ctatctacgg tcttggcggt
    14041 ctgggtaacc tcgctctgca atacgcgaag aatgtcttta acgcgaaagt gatcgccatt
    14101 gatgtcaatg atgagcagtt aaaactggca accgaaatgg gtgcagattt agcgattaac
    14161 tcacgcaccg aagacgccgc caaaattgtg caggagaaaa ccggtggcgc tcacgctgcg
    14221 gtggtaacag cagttgctaa agctgcgttt aactcggcag ttgatgctgt ccgtgcaggc
    14281 ggtcgtgttg tggctgtcgg tctgccgccg gagtctatga gcctggatat cccacgcctt
    14341 gtgctggatg gcattgaagt ggtcggttcg ctggtcggca cgcgccagga tctaaccgaa
    14401 gccttccagt ttgccgccga aggcaaagtg gtgccgaaag tcgccctgcg tccgttagcg
    14461 gacatcaaca ccatctttac cgagatggaa gaaggcaaaa tccgtggccg tatggtgatt
    14521 gatttccgcc gttaagaggc ctttgctgcg agtgccagtc ctggtgtcgc agcatcgcac
    14581 tctctccgac attaaatggc taaatggatt attgttcctt cctacgcaac aataatgata
    14641 gtgttcctga tgaaggaaca ctactgagag taaagacact gatgaaaatg gccaatcatc
    14701 cccgcccggg ggacattatt caggaatcgc tggacgaact taatgtcagc ctgcgcgagt
    14761 ttgccagagc aatggaaatt gctccctcaa cggcaagccg attgctgacc ggaaaagcag
    14821 ctttgacacc agaaatggcg attaaacttt ccgtggtgat cggcagttcg ccgcaaatgt
    14881 ggctgaatct gcaaaacgcc tggagtctgg cagaggcaga aaaaacggtg gatgtgtcgc
    14941 gactacgccg tctggttacg caataaaaaa atggcgcttc ttaaagcgcc attttcagct
    15001 taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct gccttctcga
    15061 tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc ttcccttcga
    15121 tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc aggatggcgt
    15181 ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg
    15241 ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc agcacgaaaa
    15301 tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta ccgacatccg
    15361 ccactttatg ctcattgcct ttcaatactt cgacaatgtt caacagccac ggaatatctt
    15421 tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa
    15481 tcgcaatgcc gaaaaacggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg
    15541 tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc
    15601 aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg
    15661 acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc aataccgatg
    15721 tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac
    15781 gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag accgtgatac
    15841 agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc gaccccttcc
    15901 gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac gcgctgttcc
    15961 gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc
    16021 acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc
    16081 cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca
    16141 cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca
    16201 ccagcagacg ggcacgcctt caggcagccg ggatcttcac agtgcataca gccgtcttta
    16261 cggatcagcc actccagctt gccgttctgt tcggtttcgc taaagcgcat caccgtccag
    16321 gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca
    16381 cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat acaggtggaa
    16441 acgtcgataa gttttgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg
    16501 gagttagttg cggacctttt gataatgtcc tgcgtttcca tagccatctg ttcgccccct
    16561 tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga gtttgcatca
    16621 ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc
    16681 cagtggattg gaatacccac cgtttcaacc tgctgaccat ttacattcag tggtttcaga
    16741 cgacgcgtta ccacagccac cgcgcggata aagccacgct tgcttgagac agtgacacga
    16801 tcgccattat taatgccttt cgccgccgcc agcgtttcgc tgatttccac aaactgttcc
    16861 ggctgagcaa ttgcgttgag caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga
    16921 cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag tgcgtcttgt
    16981 tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt agtgcccagc
    17041 ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca
    17101 aacaggcgtc ccatcccttc cggctgcatg ataaacggcc cggttggcgt acccggtgcg
    17161 gcattgccga agtcaggaat atcgttaccc gtccacttgc tgccgttcca ctggatcagc
    17221 atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt gtacagcacg
    17281 cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc ggacgggtcg
    17341 gagttatcgc ggttagccat ctggttgccc tgctctgtcc agctaccggt gtagatccag
    17401 caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct
    17461 ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccagcgcata gccgttgttc
    17521 tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca gctcatcttc
    17581 atcagcggtt ctacgccttt accaccttcg gcctggtaca gctcgcgcag gtgatggtag
    17641 atacccgcca gaatttcacc gtcgttacgc gcttcgcccg gcgcatcctg acctttccag
    17701 tgccactgca gccagcgacc ggagttagca atagaaccat cttcttcagc aaagcaggtc
    17761 gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt cgactcgccg
    17821 tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc
    17881 agcttgctca ggcagctcac cactttgttt ttgtccggga aggacgcaac cgggttaaag
    17941 ccctggcaga aataaccggt gactttgcct tcgtccatca tgttgaaata cttgatgacg
    18001 tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc tttctgcgcg
    18061 gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt gctccagtag
    18121 ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca
    18181 acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag cagacctaag
    18241 tcagtcaggc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata
    18301 cccatgttac cgagcagtaa ctggatcatc gccatagtac ggatgttctg cgcacccacg
    18361 gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg
    18421 gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccgca gatgttttca
    18481 acgacgtccg gcgtgtagcg ggaaacgtgc tctttcagca ggttccacac acagcgcgga
    18541 tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata gttccaggac
    18601 gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc ttcgaaagca
    18661 aaatcatcac gcaccagcag gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt
    18721 ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa
    18781 cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgagg atcgacaacg
    18841 atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc
    18901 gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat atccacccag
    18961 tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt
    19021 cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttctgtgtc
    19081 agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga aagccaacgg
    19141 tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc agctttcatc
    19201 agctttgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct
    19261 ggcgcacgat attccgggta gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg
    19321 gccccttttg ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga
    19381 taaatcgctt ctctggcgtt tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa
    19441 cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa tttgtagttt
    19501 cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt tgttccagcc
    19561 ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt
    19621 tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa
    19681 atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa
    19741 tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct ataaaattca
    19801 gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc
    19861 tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca
    19921 gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt
    19981 taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg
    20041 ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga
    20101 cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc
    20161 tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat
    20221 tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa
    20281 tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta
    20341 tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg
    20401 tttttgtcct gctaacggga gcaagtctgt gggtttacta ttttctcacg ccacaaccag
    20461 aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat
    20521 ttgcttatgc tgcatggaat gtcggtatac tgcatggcaa tgtcaccata atggcggtag
    20581 gctcgtattt tacgcccgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc
    20641 tgtcgttctc attctggcaa ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc
    20701 tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa
    20761 attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc
    20821 gacttcgatc acatatcgat aaataatatt atttcatcta tttttctcat tgtttttaat
    20881 caccat
//
LOCUS       DAZFRN010000084        20375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFRN010000084 DAZFRN010000000
VERSION     DAZFRN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.084"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(194..940)
                     /gene="cutC"
                     /locus_tag="ACHKUB_002944"
     CDS             complement(194..940)
                     /gene="cutC"
                     /locus_tag="ACHKUB_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO6655927.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(954..1520)
                     /gene="yecM"
                     /locus_tag="ACHKUB_002945"
     CDS             complement(954..1520)
                     /gene="yecM"
                     /locus_tag="ACHKUB_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6655928.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1736..3469
                     /gene="argS"
                     /locus_tag="ACHKUB_002946"
     CDS             1736..3469
                     /gene="argS"
                     /locus_tag="ACHKUB_002946"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO6655929.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3511..3555)
                     /gene="yecV"
                     /locus_tag="ACHKUB_002947"
     CDS             complement(3511..3555)
                     /gene="yecV"
                     /locus_tag="ACHKUB_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO6655930.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3646..4134
                     /gene="yecT"
                     /locus_tag="ACHKUB_002948"
     CDS             3646..4134
                     /gene="yecT"
                     /locus_tag="ACHKUB_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO6655931.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(4254..4646)
                     /gene="flhe"
                     /locus_tag="ACHKUB_002949"
     CDS             complement(4254..4646)
                     /gene="flhe"
                     /locus_tag="ACHKUB_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO6655932.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4646..6724)
                     /gene="flhA"
                     /locus_tag="ACHKUB_002950"
     CDS             complement(4646..6724)
                     /gene="flhA"
                     /locus_tag="ACHKUB_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO6655933.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6717..7865)
                     /gene="flhB"
                     /locus_tag="ACHKUB_002951"
     CDS             complement(6717..7865)
                     /gene="flhB"
                     /locus_tag="ACHKUB_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO6655934.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8067..8711)
                     /gene="cheZ"
                     /locus_tag="ACHKUB_002952"
     CDS             complement(8067..8711)
                     /gene="cheZ"
                     /locus_tag="ACHKUB_002952"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO6655935.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8722..9111)
                     /gene="cheY"
                     /locus_tag="ACHKUB_002953"
     CDS             complement(8722..9111)
                     /gene="cheY"
                     /locus_tag="ACHKUB_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO6655936.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELEFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9126..10175)
                     /gene="cheB"
                     /locus_tag="ACHKUB_002954"
     CDS             complement(9126..10175)
                     /gene="cheB"
                     /locus_tag="ACHKUB_002954"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO6655937.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10178..11038)
                     /gene="cheR"
                     /locus_tag="ACHKUB_002955"
     CDS             complement(10178..11038)
                     /gene="cheR"
                     /locus_tag="ACHKUB_002955"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO6655938.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11057..12658)
                     /gene="tap"
                     /locus_tag="ACHKUB_002956"
     CDS             complement(11057..12658)
                     /gene="tap"
                     /locus_tag="ACHKUB_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO6655939.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12704..14365)
                     /gene="tar"
                     /locus_tag="ACHKUB_002957"
     CDS             complement(12704..14365)
                     /gene="tar"
                     /locus_tag="ACHKUB_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO6655940.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14510..15013)
                     /gene="cheW"
                     /locus_tag="ACHKUB_002958"
     CDS             complement(14510..15013)
                     /gene="cheW"
                     /locus_tag="ACHKUB_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO6655941.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15034..16998)
                     /gene="cheA"
                     /locus_tag="ACHKUB_002959"
     CDS             complement(15034..16998)
                     /gene="cheA"
                     /locus_tag="ACHKUB_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO6655942.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17003..17929)
                     /gene="motB"
                     /locus_tag="ACHKUB_002960"
     CDS             complement(17003..17929)
                     /gene="motB"
                     /locus_tag="ACHKUB_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO6655943.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17926..18813)
                     /gene="motA"
                     /locus_tag="ACHKUB_002961"
     CDS             complement(17926..18813)
                     /gene="motA"
                     /locus_tag="ACHKUB_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6655944.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18940..19518)
                     /gene="flhC"
                     /locus_tag="ACHKUB_002962"
     CDS             complement(18940..19518)
                     /gene="flhC"
                     /locus_tag="ACHKUB_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO6655945.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19521..19871)
                     /gene="flhD"
                     /locus_tag="ACHKUB_002963"
     CDS             complement(19521..19871)
                     /gene="flhD"
                     /locus_tag="ACHKUB_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO6655946.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4902 a         5595 c         4956 g         4922 t
ORIGIN      
        1 tttaaatccc ataatcaatg tgaatataaa aaatattatt agcacaacgc tatgttcact
       61 gtcacccgct caacagatca cggttatcgt tcgtttttta tactgctcag ggataaaaaa
      121 aggcccctgt tgaaattgca ggggcctggt acgagcaagc atcatattgg gcgacatgat
      181 gcaacggtaa aaatcatttg gcctgatggc gttcaatgat tcctttcatt tcagcaaccg
      241 ccgccccgtc tacgatataa cgcgaatact cgtccgcgtg ttcatctgat gacatggaca
      301 atccttgatt acgataacgc atcggtgagg cttgccacgc tcccgcggag ctatggactt
      361 ccagcactcc ggcatcgagg aagtggtgca ggttttctgc acggactcct gctccggcca
      421 taatgattgg agcatcacga tgggcaataa gttccataat ttttgataaa ccttgcagtg
      481 cgtctgattt ttgccctgat gtcagcaccc gggcaatgcc caattccgca agattattga
      541 gtgtatataa agggttagcg cacatatcga aggcgcgatg aaaagtcact gccagcggac
      601 cggcagcagc cattattttt tccattcgtg gcatatcgac attcccgtca acatcgagaa
      661 cgcccgtcac cagtccagga aaacctaatt cgcggaccgt gcgcacatcc tcaagaatgg
      721 cggcaaactc accgtcgctg tagcaaaaat caccaccgcg tgggcgaatt atcggatgca
      781 cagggatcgt cacccgctgg cgcacggatt tcagtacccc caacgacggc gttaagcccc
      841 cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc gccgttagtg
      901 cacattccat gctgtaacag caaatttcca gtaatgccat ttttgctcct taattacgct
      961 gactgctcgc tggcaacgat ctcttcaatg gaccacggat gaaacttaat agttgttttg
     1021 ccatcggtga ccgccagcgt cggattcggt aatcgctcat gctcaccttt tggtgaactg
     1081 gttttaactg agatccccgg taggctgagt ccttcatcag aaagcaatgc cagcgcacgg
     1141 gcattcagcg tttctggatc acccggcaga acgatttcaa tatgttccca accttcgtgt
     1201 gggtaacgtt tttccccggg ccacggtagc tccacaatag aaaactgcca gtgcgcaacc
     1261 tgtaccggtt catgcaattt aaacagacaa atcggtctgc cattgatcat attttctgac
     1321 aaaagctcgc cacactgttc aaacccgcga cgccagcgtt cagcagtggc gttttgatgg
     1381 caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat tcaaccccag acggagggaa
     1441 agttcatcta atgcgtgggt aaatcgcggt aaatccgatg caatatcctg cagctcgtcg
     1501 atagattgcc agttcgccat aatcactctt cgtctttcag taaaagcgtt aatttaccct
     1561 gttgccctgc gccaaccaac cgctgatttc acgccgcttc tgatgcaata gtgaaaacgg
     1621 caatacgcca cgcgcacgtt gctgacgaaa acagccattt gcagtatact cccgccctaa
     1681 tttctttaac tggtgcgggc aatttttgct cgcttcatca atgtaaggta ttccggtgaa
     1741 tattcaggct cttctctcag aaaaagtccg tcaggccatg attgcggcag gcgcgcctgc
     1801 ggattgcgaa ccgcaggttc gtcagtcagc aaaagttcag ttcggcgact atcaggctaa
     1861 cggcatgatg gcagttgcta aaaaactggg tatggcaccg cgacaattag cagagcaggt
     1921 gctgactcat ctggatctta acggtatcgc cagcaaagtt gagatcgccg gtccaggctt
     1981 tatcaacatt ttccttgatc cggcattcct ggctgaacat gttcagcagg cgctggcgtc
     2041 cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt gtggttgact actctgcgcc
     2101 aaacgtggcg aaagagatgc acgtcggtca cctgcgctct accattattg gtgacgcagc
     2161 agtgcgtact ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg tcggcgactg
     2221 gggcactcag ttcggtatgc tgattgcatg gctggaaaag cagcagcagg aaaacgccgg
     2281 tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga
     2341 agatgaagag tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga
     2401 atatttccgc gagatgtggc gcaaactggt cgacatcacc atgacgcaga accagatcac
     2461 ctacgatcgt ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa
     2521 cccgatgctg ccaggaattg tggcggatct caaagccaaa ggtctggcag tagaaagcga
     2581 aggggcgacc gtcgtattcc ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt
     2641 gatcattcag aagaaagatg gcggctatct ctacaccacc actgatatcg cctgtgcgaa
     2701 atatcgttat gaaacactgc atgccgatcg cgtgctatat tacatcgact cccgtcagca
     2761 tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt
     2821 accgctggaa caccacatgt tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac
     2881 ccgcgcgggt ggtacagtga aactggccga tctgctggat gaagccctgg aacgtgcacg
     2941 ccgtctggtg gcagaaaaga acccggatat gccagctgac gagctggaaa aactggctaa
     3001 cgcggttggt attggtgcgg tgaaatatgc ggatctctcc aaaaaccgca ccacagacta
     3061 catcttcgac tgggacaaca tgctggcgtt tgagggtaat accgcgccat acatgcagta
     3121 tgcttacacg cgtgtattgt ccgtgttccg taaggcagaa attaacgaag agcaactggc
     3181 tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca
     3241 gtttgaagaa accctcaccg tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta
     3301 cctgtacgat ctggctggtc tgttctctgg cttctacgag cactgcccga tcctcagcgc
     3361 agaaaacgaa gaagtgcgta acagccgtct gaaactggca caactgacag cgaagacgct
     3421 aaagctgggt ctggatacgc tgggtattga gacagtagag cgtatgtaat cgatttttcg
     3481 tgagagtaaa gcctgatcag ggttacctga tcaggctttt ttattgccat ctaaatgtat
     3541 tctgaaaatg gacatgccat tgttttctga ctgttggata agaggccaga agcgtaatat
     3601 ccggccccag ggaaacgata acggttgaat ttaaggaata ccgcagtgtt taaatttctt
     3661 gtattaacat taggcattat ctcttgccag gcttacgcag aagatacggt tatagtaaac
     3721 gaccatgaca tttcagccat caaagattgt tggcaaaaaa attcagatga tgatactgac
     3781 attaacgtga tcaaatcatg cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat
     3841 aaagcctatg gtgaagctta tcgttatata gaacaagtgc cacgcacagg tgtaaaaaaa
     3901 cctgataccg aacaacttaa cttgcttaaa aaatcacagc gagcctggct ggattttagg
     3961 gacaaagaat gtgaattaat cctttcaaat gaggacgttc aggatttaag tgacccttat
     4021 tctgaatcag aatggctctc atgtatgatc atacagacca atacgcgaac tcgccagttg
     4081 cagctatacc ataactctga agatttttat ccaagccctt tgacaagagg ataattcaca
     4141 tctttttggc atgttttgtt gcaagctatt cctgataaat aattgcaaca agacatcgag
     4201 cctttttcac tgagttatta aacatactcg cgagcgcgta atttttttgt ccttcagcga
     4261 taattcacaa tcacttcatt acgttgtacc tttagcggtg gaattaaccg cccaccgcct
     4321 ggcacttccc agataaatcg caacggttct gctgccgcta taccggaaaa ggccacggtg
     4381 gttccgctct gcccctctaa ttcgacacaa cgagattgcg aacacaagcg aacccgcagt
     4441 cctgaaggtg tcgggccgat aagctgataa cgccacgcta ccagcgtcat caatcctgaa
     4501 gcaggttgtc gcgtagaaag aggcgcagac gacatcgact caccgcgatg atttagcgta
     4561 attcctacac tacttgcctg ccacatcccc tccccggcgg cttgcaccag cagcggaaac
     4621 aataatattg ctaataaggt tctcattatt tgccgccaat tgtcgccgtc atgcggatat
     4681 gtcggttatc agacagttcc agattcgaca ggaccactaa ctgcggcaag ctgcggcgca
     4741 ggaagcgaga caataatggt cgcagcgcgt ggttcaccaa caatactggc ggcgcaccca
     4801 gcatctcctg acgggatagc gcttcctgag tttgcgccag taaacgatcc gccagccctg
     4861 gctccagtcc tcccccgccc tgcagcgcct gtagtaacaa acgttccagc ggtgtatcga
     4921 ggccaataac atggacttca tctttgccag gaaaccactg ctgggtaatc gcccgtccca
     4981 acgccacgcg cacgacggcg gttaattcat gtggatcgct ttggatgggc gcatgttccg
     5041 ccagcgtttc gagaatggtg cgcatatcgc gaatcggtac tttttcatcg aggagatttt
     5101 gcagcacttt atgcagtgtg gtgagcgtga cgacgccagg aacgagatct tccgtcagct
     5161 ttggcatctc ctgggcgacg cgatccaaca gctgttgcgc ctcctgacga ccaaacagct
     5221 ctgcggcatg ctggctaatg aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa
     5281 ccactgtgta cccctgaatc tgcgcctgtt cttttagcgc actttcaatc cagatagcat
     5341 tcaggccaaa tgccggatcg acggtcgcct caccaggtaa cgtcccggca gcggttccag
     5401 ggttaatcgc cagccagcgc cccggataag catcaccact gccaatctcc acgcctttca
     5461 tcaaaatgcg atagcgggca ggttgcagat ccatattgtc gcgaatgtgc accactggcg
     5521 gcagaaatcc catctcctgg gcaaatttct tgcggatact gcgtatacgg cccaacaact
     5581 caccatcctg ctggaaatcg accatcggga tcagtcgata acccacttcc attcccagag
     5641 aatcttccag ttgtacatcg ttccacgtcg cttcgacaac ggtattattc tctgccattt
     5701 ttaccggttt gggttcgaca ggcgcttttt gttcgcgtcc gcgtatccac caggccagcc
     5761 ccagcaatcc ggcagtgaac agcaaaaata ccaggttcgg cattccaggc accaggccga
     5821 gtaaaccgag cacggcggcg cttaacaaca taacgcttgg gttactgaaa agctgattca
     5881 ccatctgctc gccaacatcc tgatcggtgc tgacacgcgt aacgatgacc cccgcggcgg
     5941 tagaaatcac cagcgccgga atttgtgcca ccagaccgtc gccaatggtc aatagtgtat
     6001 aactttccgc cgcgtgtccc atgctcatgc catgttgcag cacgccgacc agcaacccgc
     6061 cgacaacgtt aatgaccatg atgaggatcc cggcgatggc atcgccgcga acaaacttac
     6121 ttgccccgtc cattgagccg taaaaatcgg cttcctgagt cacttcggag cggcgttttt
     6181 tcgcctcatc ttcaccaatc aatccagcgt taaggtcggc gtcaatcgcc atctgcttac
     6241 ccggcatacc atcgagaaca aagcgcgcac ccacttctgc gatacgcccg gcacctttgg
     6301 taatgaccat aaagttgatg atcacgagaa tgacaaacac tacgataccg atagcgaaat
     6361 tgccaccaac gaggaagtga ccgaatgctt cgaccacctt cccagccgcc gccgcgccgg
     6421 tatgcccttc catcaaaatg atacgggttg aagccacgtt aagtgccaga cgcaacaacg
     6481 tagtaaacaa cagaatggtc ggaaacgcag caaactcaag cgtgcgctgg gtaaacatcg
     6541 ccaccagcaa caccatgatc gacaaggcaa tattgaaggt aaacaacagg tcgagtatga
     6601 atgcgggcag tggcagcacc atcatcgaca agatcaacag gatcaaaatc ggtccggcaa
     6661 ggatctgcca ttgtgtcgat ttcaggtttg cgggcaggcg cagcatcgcg gccagattac
     6721 tcatgggtcg gtttctcgtt aataaaatcc agggcttccg gcaccggaag atgagtaggt
     6781 tgtacagggc gctgtccacc agccagacgc cagcgtttca gttgccagac ccaggccagc
     6841 acttccgcca ccgcggcgta cagttgaccc gggatttgtt gaccaatctc cgcatgtcga
     6901 tacagcgctc gcgccagcgg cggcgcttca agcgtcggga cgttattttc agcgccaatt
     6961 tcacgaatgc gcagcgcgac cagccctgca cctttagcga ccactttcgg tgcgctcatt
     7021 ttgttttcgt catactgcaa cgctaccgaa tagtgggtcg ggttattgac aatgacatcc
     7081 gctttcggca catcggccat catccgacgc cgtgcagcag ctcgctgcat ctgacggatc
     7141 cgccctttaa catgagggtc accttcgctt tgtttgaact catcacgaat atcctgccgt
     7201 gacatacgca gctttttcag gtggctgaat atttggaaaa agacgtcaaa tcccaccatt
     7261 ggaatgacac caagcaccac cagcagtgcg catagcccta ccaaatccat cgcattaccc
     7321 atggcggtaa tcggagactc ggccatcaag cgcatcatct gcggccagtg atgccagaga
     7381 aaaaaccccg tcacgctgcc aaccaggatg gttttcaaaa ttgctttaag caactccgcg
     7441 ccagtctgag ccgagaacat ccgtttaatg cccggtagcg ggttgagttt ggaaaacttc
     7501 ggctgcaagg atttgccgct aaataccagc cctcccagca tgaccggaga aatgagcgcc
     7561 accagcacca cgccgctaat cagcggcagc agcgccagca tggcttctct gatcagcaga
     7621 ataatctgcc cgaggatcag attcgggtca ttgataatac tgtgatcaaa atgcagccca
     7681 gcggagagca tgcccgacaa tcgacgggcc agcgacacac cgccaaacca gataacacta
     7741 acgcccacca gcaaaatcag cagtgaggtc agttcacggg aacgcgggat ttgcccctct
     7801 tcccgcgctt tttctagtcg gtgaggtgtg ggggcttctg ttttgtcgtc gctctcgtca
     7861 gacacgtcgc caatcctgga aagagtatta agcgtgaatg atgccagagc gcaaagcgtt
     7921 caatggtttg agtaaggggc aaaacaggcg ggatttaggg cttttgctgc cacacatcaa
     7981 gcatagtgtg cgtttgtcgg atgcggcgtc atcgccttat cagaccgcct gatatgacgt
     8041 ggtcacgcca catcaggcaa tacaaatcaa aatccaagac tatccaacaa atcgtccacc
     8101 tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc attaagcaaa
     8161 ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa cagcaccatc
     8221 agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaatgac ctgcccggtg
     8281 agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt aaagctggta
     8341 tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg ggcgtcggca
     8401 aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt tgctgatttc
     8461 tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg ctccgcagcc
     8521 tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat ggcttccgcc
     8581 gcttcggcaa tggcctgatc cagccccagt tcccgcaaac tgtcgcgcag catacgcgtc
     8641 aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg tttgattgat
     8701 ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt tgagtttttc
     8761 ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg cttgcgccgc
     8821 agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca atgccgacat
     8881 cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg gcatgttcca
     8941 gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt cgacgccatc
     9001 ttccgcttcc tcaacattat tgaattccag ctctttcagc aggttacgca ctatgcgtcg
     9061 catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca tttcacactc
     9121 ctgatttaaa tacgtatcgc ctgtccggca ctaatttttg ccaacatttg ctggcttacc
     9181 tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg cggcatgccg
     9241 aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg acgcatcgcc
     9301 aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac cgcattacgc
     9361 cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg acggttaacc
     9421 gccgggccat cgtgaatttt gatttggtaa tttgcgccac tacgcgacag ctccatatgc
     9481 cgatcgcccg gcgcaatata ggcatgcccc ggcaagacac gttctccgtc ttcggcttct
     9541 ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt gaaaccgggc
     9601 ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg cagtacgtga
     9661 cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc agaactcaac
     9721 aacggacccg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc aaggctcgcc
     9781 tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat tccttcgcga
     9841 atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc gcgcagcgtg
     9901 acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg acgcaaacgc
     9961 attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc cagcgtcagc
    10021 acatcgggat tgaatttctt aatcaagtca cgcgcgacca gcggatcagg cgcggtcgcc
    10081 accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg catcagtgcc
    10141 gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt agcgcataca
    10201 ccgtctgacc acgcagcgtg aagcggcgct caaggtggct aaagttttca gagtgacccg
    10261 caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc tcctgctggg
    10321 tattttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac ggccccggca
    10381 cggtgtactg tttcgccagt agattcagcg gggcaaaatc aacatagttc gccagctcct
    10441 gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac cgttgcagtt
    10501 gctgcggcgt caggtttttc aactcttcat ggcgataaat accgcttctg gctttttcca
    10561 gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcgcg gtgcccaatg
    10621 tgtcagccag cgtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc gccgcgctcc
    10681 atacgcgata ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg aaatggtgtg
    10741 cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc tgccactcac
    10801 cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc agtcccagcg
    10861 aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca gccagaacga
    10921 tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg gaaagcgcca
    10981 ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat gaagtcatag
    11041 cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc gactcatgtc
    11101 gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga tggtcggcct
    11161 gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggcg ttctgctgag
    11221 tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc tgttgttctt
    11281 ccgacgcaga ggcaatttct cccataatgt cgttcacgcg ggtcaccgaa ctgacaatat
    11341 cgatcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca cgattgactg
    11401 actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg gcaagattgc
    11461 gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga gccgcttcca
    11521 ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata acgctgataa
    11581 tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc atggtactga
    11641 cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc gacgcctgtc
    11701 gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg gcggccgttt
    11761 gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg cctgcgacaa
    11821 tctccgcgat accaatgtgc atctcctggc ttcccttacg cacatcactc accgtcccac
    11881 gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca ttacgaccat
    11941 ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg ctaccgataa
    12001 tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg atgtagatcg
    12061 ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta cgctggcttt
    12121 gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca aagttaacgt
    12181 caaacgcatt ctgtgattcc tgcaccggcg cagtgaggaa atccgaaagt tgattacttt
    12241 caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt ttctccgtct
    12301 ctttttgcaa cgccctgacg tgctcgttgc ccgcagtcat cgccataaaa cttttaaaca
    12361 gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta atgtcatccg
    12421 ccggatagct aagtgccgtc agagtgcccg ctttgttcag cgcggtactg gcctgtaaca
    12481 ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg acctgattca
    12541 gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca atctgcaaga
    12601 tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga ttaaacatca
    12661 acgctcccca tcaggcggca atgaccgcgt tagtaaatac tcgtcaaaat gtttcccagt
    12721 ttggatcttg ttcagcaatt cgcagtcgtg gctgtgccgg tggttgctca ctggcaggac
    12781 gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac gcggaaaccg
    12841 cttgcgttaa acgactcgcc tgttcttcca gcgcagcggc ggcggcagct gattcctgca
    12901 ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat gcgacttgat
    12961 cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg tcagtcacgc
    13021 gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc agcaccgaac
    13081 cggtatcaac gcgtgagacg gagtcttcaa tgagggcttt gatctctttt gccgcctggg
    13141 cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca cggccctgtt
    13201 cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc tggaaagcaa
    13261 taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg atctcatgca
    13321 tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg gaggcacttt
    13381 gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc gcggtgagct
    13441 gctccatgct ggcggcagtt tcttccagcg cggatgcctg ctgttcagta cgggaggaaa
    13501 gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct gaaccttcgc
    13561 ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc tgcgccaggt
    13621 cgcccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca ccggcgattt
    13681 cgcgaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga atgccgtacc
    13741 acgccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc cactgggcaa
    13801 atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca ctgctgaggg
    13861 cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc tgagcgaaat
    13921 aagctccagt attgccataa tcaagataat caatcagttc agttaacgct gtgtgatagt
    13981 ttttatattt ttcatcaata ttacgactgg tggcgaccat ttcaggtaac ggtgccatgc
    14041 ttttgaattt tttataatgc gtcgcggcct gggccaatgt tttcctggcg ctatcgagca
    14101 attcaacttt ggcattactt tgttgattgg aggaatccat catcatccgc accgctgaac
    14161 gactcaggtt aatacgcgtt tgcagcatta aatcccaggt tgacgtcagc tcgccctgct
    14221 gttcccgtaa ttgattggaa accacaaagc tcttctggct atggtgaagg gaagaaaaaa
    14281 acagactgcc ggaaataagt tgtaacagtg cgaatacccc cagcaccatt accaacagcg
    14341 tgactacgcg gatacggtta atcataaggc accttcctga aaacaagttg atctcgttat
    14401 cggcaagaag gggagaaact ttattgctga tgccacccgc cgcgaaattg aaataaaaaa
    14461 cccgatgcgc agatcatcgg gttcatttca attgaggaaa tcgggagaat tacgccactt
    14521 ctgacgccgc gctatctaac agcgccatct cttcgctatt cagcagtttt tcgatgttca
    14581 ccagaatcaa catacggtcg cccagtgcgc ccagtccagt gagatattct gttgaaagcg
    14641 tcacggcaaa ttccggtgcc ggacgaattt gctccgccgt caatgaaagc acgtctgaaa
    14701 cgccgtcaac tacgatgccg accacccgct gtccgagatt caggacgata actaccgtgt
    14761 tgtcgttata gtccacatcc acctggctga acttaattcg taagtcaaca atcggcacaa
    14821 taaccccgcg cagattcgtg acgcctttga taaacgctgg cgtgttcgca atccgtgtta
    14881 cctgatcgta gccacggatc tcctgcactt tcaggatatc aataccgtac tcttcatcac
    14941 caagggtaaa taccagaaat tcctggcctg acggctcgct ggccagcttt gttacattcg
    15001 tcataccggt catattgtta cctttttact cattcaggcg gcggtgttcg ccatacgttg
    15061 ttcgcggttt atcgcctgca aggcggagac atcaacaatc agtgccacgc tgccgtcgcc
    15121 aagaatggtc gcagcagaaa tgccggggac tttgcgatag ttactttcaa ggtttttaac
    15181 cacaacctgg tgctgaccaa ttaattgatc caccagcaag gcgtagcggc gaccgccact
    15241 ttgtaagatc accacaattc cctgggtggc ttcggttttc gcgcccgcga cgttgaacac
    15301 tttccacagt tcgacgatgg gcagatattc gccccgcact tccagcaccc gctcgccgcc
    15361 ggccagtgga tgcagatcgg cttcacgggg ttgcagtgat tccatgacag cattcagcgg
    15421 cagaatgaaa acttcatccg caacgcgtac ggacatgccg tcgaggatgg ccagcgtcag
    15481 cggcagtaaa atgcggatcg tagtgccagt accctgcttc gactggattt cgacatgacc
    15541 gcccatctcc tggatattac gtttaacgac gtccatgccg acgccgcgcc cggagacgtc
    15601 ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc agcatcgcga cttcgtcgtc
    15661 gctcatgttt tcgctgacag tcaaaccttg cgaggccgct tttgccagaa ttcgctcacg
    15721 gtttagcccc gccccatcgt cggtcacttc aatgcaaatg ttgccgccct gatgttcggc
    15781 agacagaatt aaatttccga cgctgttttt acctgcggcg agccgttttt ctggcagttc
    15841 aataccgtga tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa tgcgttctat
    15901 caggctcttg tcgagctcgg tggaactgcc caccagcgtc agttctacct gcttgccgag
    15961 ttttcccgcc agatcacgca ccagccgggg atagcgacta aagacatatt ccatcggcat
    16021 catgcgaatc gacatcactg attcctgcaa atcacgggcg ttacgttgta actgccccat
    16081 gctggtaatc aaatcaccat gattaaccgg gtccagttcg ctggaacgct gggcaagcat
    16141 ggactgggtg ataaccagct cgccgacgag gttaattaat tgatcaacct tttctaccgc
    16201 tacacggatg ctggtggatt cactgctgcg cgtcgttttt tcccgctcca cgcgaccggt
    16261 tggcgcttgt tcggctgcca gtttaagcac tggtggggtg gatatttttg gcgagacttc
    16321 tactgtttca aaggtaatct gatcggcttc aatcacaaaa cagagtaccg ctgtgatgtc
    16381 atcttcggcg atatcgcccg gtaatattgc cgagagagaa tccgcccctt tcaccacgtc
    16441 agttaacgtt gtcagatgcc ccagctcttc ttccagcagg tcgacttcgc cggccttcag
    16501 gcgcgaaagg ataattcgtc gcggcgactg actgcgactc tgctcatctt gcggttcact
    16561 tttggcaacc acacttaatc gggtcactgc ggatggcgtt tcgcctttcg cttctaatgc
    16621 cagttgacgc aaggcctggc agatataatc gaagctggcg gcatccggct cttgcgactg
    16681 tttataagcg tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca gattgataat
    16741 gtcggtgttg agttgcatct cacctcgtct ggcttcatcg agcaggtttt ccatcagatg
    16801 cgtggtttcc tgcaaaacgc tgaagccaaa agttcctgcc cctcctttga tcgagtgggc
    16861 agcccgaaag atggcattca attgttcggc atctggcgct tccggctgca aaaccagcag
    16921 atgctgctcc atatcagcca acagttcgtc cgcttcatca aaaaatgtct gataaaaatc
    16981 gcttatatcc atgctcacgc tgtcacctcg gttcggctga tggcattgtg ggaacactga
    17041 cctgtggtgc aacctcaggt ttttccaggg cgcttactgg ctcattctgg ctttcggcgt
    17101 tttcatgcaa aatggcctgt tcggcttgtt tgttcagtac cagcaggctg atgcgacggt
    17161 tgacggcatc atcaggtccg cgatcgctta agcgcatcgt tgccgccatg ccgacgacac
    17221 gtaacacttt gccgctatcc aaccctccga ccatcagttc gcggcgggat gcattggccc
    17281 gatcggcaga aagctcccag ttgctatatc ctttctcacc gctggcgtag gggaaatcat
    17341 cggtatgacc tgaaaggcta atacggttgg gaataccgtt cagtacaggc gcaatggcac
    17401 gcagaatgtc gcgcatatag ggttcgacat cggcactgcc ggttctaaac atcgggcgat
    17461 tctggctatc gatgatctga atacgtagac cttcctggac cagatcgatt ttgagatggg
    17521 gacgtaacgc ccgcagtttc ggatcggact ctatcaactg gtcgagatca ccccgcaatt
    17581 tccgcaatcg actttgctcc atgcgttttt tcagctcttc gatgttcggc tgcttattca
    17641 cttccccctg gctttgggtg taatcatcac caccgccggg aattgggctt tcactattag
    17701 aaatgcgatc gccgcccgta accgcagtcg ccagtggagt ccggaagtac tccgcaatct
    17761 gaatcagctc ttttgggctg gagatggaga tcagccacat caccagaaaa aaggccatca
    17821 tcgcagtcat aaagtcggca taagcaatct tccacgatcc atgtgctgcc ccgtggcttt
    17881 tggctttgcg tcgtttgacg acaataatcg gatgcgcttg attcttcatg cttcctcggt
    17941 tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc tcttccagtt caataaacga
    18001 cggacgttcg ctggaataga gcgttttgcg accaaactca acggcgatag gcggtgcgta
    18061 accgttcaga ttagaaagca gagtgacttt gacgcactgc atcattttgc tggtttcggc
    18121 gcttttctga cgtaaaacag tcgctaatgg ggaaataaat ccgtaagcca ataaaatgcc
    18181 gaggaaagtc cccaccatcg catgtgcgat aagcgcaccc agctcggcgg caggacgatc
    18241 ggctgaacct aacgcgtgaa cgacccccat tacagccgcg acaataccaa acgccggaag
    18301 tgagtccccg accagcgcca gactgttcgc cgggacttct gcctcgcttt cgtgcgtctc
    18361 aatctcttca tccatcaggg cttcgatttc gaaggtgttc atatgaccgc tgataatcag
    18421 acgcagataa tcgacgataa aatcaagcat gacgctatcc gcgaggatgc gtgggtagct
    18481 ggcgaagatc tcgctctcac ggggattttc aatatcacgt tccagcgaaa acatccccat
    18541 ctgccgcgat ttcgccatca accgataaag cagagccagc agatccatat acattgcttt
    18601 ggtgtatttg gagcgacgaa acagcaacgg cagcgccttc agcgtgcctt taatcgcttt
    18661 gccattattg ccgacgataa acgacccaat ccctgcaccg gcaataatca ccagttcagc
    18721 gggttgatag agtgctccaa ggcttccacc ggtcatcaaa taaccgccga aaactgtacc
    18781 gagaacaacc aggtaaccta ataagataag cacgacatca tccttccact gttgaccatg
    18841 acaggatgtt cagtcgtcag gcgttaacgc gcgattgggg caaaaaaaag cagcggtacg
    18901 tcgttaccgc tgctggaatg ttgcgcctca ccgtatcagt taaacagcct gtactctctg
    18961 ttcatccagc agttgtggga taatatcggc aggattctgg gaaagtttac gtctttttac
    19021 tgcccgggat ggcggttgac ataagctgca ggcaaagctg ccaacaggct ggtgagcgtg
    19081 ggtaataaaa ttgccgccgc agcagttgca gctggaaagt tgcagtaatc cactttcaac
    19141 aaaccgcacc aatgtccagg cacgggttaa tgccagcagc ggtccttctt ctgcttgcgg
    19201 gcactgttca aggtataaac ggtaggcttt gatcaccgca tcgacgccat tacacaaacc
    19261 ggttttcagt aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt tttgttccca
    19321 ggtcataaac cagtcggttg agaatggcag catgcctttc ggcggtgggc ttccgcgcag
    19381 ttctttataa agttttatca ggcgtccgcg acttaactgt gtttcgcttt ccagcatctg
    19441 caaacgagcg cccagggtga tcaattccat tgccagctga atatcccgcg cttcctgaac
    19501 aatgcttttt tcactcatga tcaggccctt ttcttgcgca gcgcttcttc aggctgatta
    19561 acatcattca gcaagcgtgt tgagagcatg atgccggtat gaatttgctg gagatcgtca
    19621 acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg gaagtgacaa
    19681 accagctgat tggtttctgc cagcttaacc atttgcggaa gagtcagtgc cgctaatgtt
    19741 gtcgccattt cttcatttat gccgagacga aacatagcgg acgctttgtc ctgaacaatc
    19801 agacgctgtg caagtagtaa atatgacaag ttgatgtcat aaatgtgttt cagcaactcg
    19861 gaggtatgca ttattcccac ccagaataac caactttatt tttatgcggt ttcaccgcac
    19921 cccgtgatgt cgccgggaag ccccggtaaa aaataattag cattggaata gttgcgataa
    19981 gctgcaataa gcagagccac ctttttggtt taataagtcc ttacaaatag aagggggtct
    20041 ttacacttat ctaagatttt tcctaaatcg acgcaactgt actcgtcact acacgcacat
    20101 acaacggagg ggggctgcga ttttcaataa tgtgtgatgc agatcacaca aaacactcaa
    20161 ttacttaaca taaatgtata agtcatactt ttgttttggg tgtatttcaa tctgttaaaa
    20221 agtttttcgc tacgctagca agcaaaaatg aaacaggaat aatcgaaatg ggatgttgcg
    20281 cacagttaaa ataactcacc gtaaataatc atctgctata aataatcact ttcatgcaat
    20341 accagataag ctatttttaa acagacactt accgc
//
LOCUS       DAZFRN010000085        20133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFRN010000085 DAZFRN010000000
VERSION     DAZFRN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.085"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            342..566
                     /gene="hha"
                     /locus_tag="ACHKUB_002964"
     CDS             342..566
                     /gene="hha"
                     /locus_tag="ACHKUB_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO6655947.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(898..1095)
                     /locus_tag="ACHKUB_002965"
     CDS             complement(898..1095)
                     /locus_tag="ACHKUB_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655948.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            1294..1863
                     /locus_tag="ACHKUB_002966"
     CDS             1294..1863
                     /locus_tag="ACHKUB_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6655949.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            2031..2414
                     /locus_tag="ACHKUB_002967"
     CDS             2031..2414
                     /locus_tag="ACHKUB_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6655950.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIQLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAE"
     gene            2411..2824
                     /locus_tag="ACHKUB_002968"
     CDS             2411..2824
                     /locus_tag="ACHKUB_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6655951.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCERLF
                     HGKKFCEACGTGIYCRAVCTKSVYRYQPDLLRNPE"
     gene            2830..3009
                     /locus_tag="ACHKUB_002969"
     CDS             2830..3009
                     /locus_tag="ACHKUB_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655952.1"
                     /translation="MQHNCLITPDTPLPDPDVVRRFVLRTVVQWLCLLMLLFMRHSDR
                     GDIDKHHLRANSYTE"
     gene            3130..3753
                     /locus_tag="ACHKUB_002970"
     CDS             3130..3753
                     /locus_tag="ACHKUB_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6655953.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            3842..4075
                     /locus_tag="ACHKUB_002971"
     CDS             3842..4075
                     /locus_tag="ACHKUB_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655954.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(4095..4304)
                     /locus_tag="ACHKUB_002972"
     CDS             complement(4095..4304)
                     /locus_tag="ACHKUB_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6655955.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKAPPASNHTA"
     gene            4802..5299
                     /gene="tssB"
                     /locus_tag="ACHKUB_002973"
     CDS             4802..5299
                     /gene="tssB"
                     /locus_tag="ACHKUB_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO6655956.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PEQVARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
     gene            5320..6864
                     /gene="tssC"
                     /locus_tag="ACHKUB_002974"
     CDS             5320..6864
                     /gene="tssC"
                     /locus_tag="ACHKUB_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO6655957.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPA
                     LYDTPDATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            6880..8217
                     /gene="tssK"
                     /locus_tag="ACHKUB_002975"
     CDS             6880..8217
                     /gene="tssK"
                     /locus_tag="ACHKUB_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO6655958.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSDRSLVDIVLA
                     LPLLNANGGNLDDGRESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSMPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            8214..8867
                     /gene="tssL"
                     /locus_tag="ACHKUB_002976"
     CDS             8214..8867
                     /gene="tssL"
                     /locus_tag="ACHKUB_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO6655959.1"
                     /translation="MNKPVISRAEQIFYPGWLMVCQLRSGQPVEDGKALYRRACQLVK
                     QAREELAEAGFSQENSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIMTLAGLWCVLSSVLADQVARLTGQG"
     gene            8870..10570
                     /locus_tag="ACHKUB_002977"
     CDS             8870..10570
                     /locus_tag="ACHKUB_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6655960.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVVAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLLACDAASRLRCVSELLWLNAMLPWYNRVTDALTDICRQEHLYFRPVAVGFCLTSVA
                     ARPGNIWQQQLTSLTALPPSAGVSSQVLPLPDILLSGIPRHRGLSHWHRLCRQTGLIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSELKTTALQRLQADSQLLNDWQ
                     QHGVPQRYGLGLYQGMWLIPPVERATGEWVAPLPPRPVIQKVTQTPKTVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQADACRLPDITRAP
                     "
     gene            10605..11096
                     /gene="hcp"
                     /locus_tag="ACHKUB_002978"
     CDS             10605..11096
                     /gene="hcp"
                     /locus_tag="ACHKUB_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO6655961.1"
                     /translation="MAIPVYLWLKDDGGADIKGSVDVQDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            11265..13922
                     /gene="tssH"
                     /locus_tag="ACHKUB_002979"
                     /gene_synonym="clpV"
     CDS             11265..13922
                     /gene="tssH"
                     /locus_tag="ACHKUB_002979"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO6655962.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRRRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGLWPLLTLSHSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     TLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAA
                     NLLKPALARGELRTIAATTWSEYRQYLERDAALERRFQMVRVDEPDDETACLMLRSLK
                     SRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQL
                     TRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARERE
                     LTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGVPLSS
                     LMKDEQTELLHLEKDIGRRVVGQDVALGSIAQRLRAAKTGLTSGNGPQGVFLLVGPSG
                     VGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAV
                     RKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDL
                     LMQQLSEKPETTESELHELIRPRLRDHFQPALLARFRTVIYRPLTQSAMRTIVEMKLA
                     QVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHM
                     AAKQKPQALALAWSDEDGMVIELRQECAL"
     gene            13919..14485
                     /locus_tag="ACHKUB_002980"
     CDS             13919..14485
                     /locus_tag="ACHKUB_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO6655963.1"
                     /translation="MTGYGRNQDAGAGKNQREEGSLRLLTPGEIALARLVFRSTIPYE
                     KVWIHHDSFLPFGLQNKQTAMVPDGKIYFREHYRDDYSLSVPFYQHMFIHEMAHVWQR
                     EKGMNVIGRGMFSWAVNYRYVLDGRLLSEYPMEQQAQIIADHFTLQAEGYGTWCDMRR
                     DGDITLDGNMSEYVIRSLYTSTLRGFPW"
     gene            14479..15021
                     /locus_tag="ACHKUB_002981"
     CDS             14479..15021
                     /locus_tag="ACHKUB_002981"
                     /inference="COORDINATES: protein motif:HMM:NF045617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative T6SS immunity periplasmic lipoprotein"
                     /protein_id="HFO6655964.1"
                     /translation="MVKIRAFRYRSALLLCLTLLTGCPGRGDYYRFDETVTVSTRGDN
                     VCFSVPEPEDYQPVSVSIAPRGTRFRERQITSEPSLRIVNGQLCIPPSFCLFPDKGQF
                     IVRSVLYSERDKDTPRIMVAGIEIADGCIFDIPLNDMEVVRPYGELKSSNVQTDQSER
                     HGPCKYPFRAVRGVINHESH"
     gene            15008..17539
                     /locus_tag="ACHKUB_002982"
     CDS             15008..17539
                     /locus_tag="ACHKUB_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO6655965.1"
                     /translation="MNLTDSLQNVLSGVGTLNRYRLDIPSCPSSLDVEDFSGTEGISK
                     IYRYDIIFTSTDRYPDAAWFLRKSATLIMGTGLLESLTEQKKVHGVITDFRRISGSED
                     QAQYRITLKPFISLLDKQFRTHRFFVNKSVPEVVEQILTEHGLKGWEYEFSLKRTYPK
                     REQINQYQESDLRFIQRLLAEVGIFYFFTLHPDAQTEVIHFGDAQAALIFDKTLPVNS
                     PSGMSDSGTDSVWALNVEHRVVESRVITNDYNHREAQNILMSVPADMTRGEGYDTSYG
                     EVYHYRPRHTERGDKIDPAPETANFWARLDHERFLAEQTRITGKSTDASLLPAQVLTI
                     TDSTPPVLPAPLQEPVLLTQLLFTGSRKSALQVMLSAVPYSEIVCWRPPLLTRPKITG
                     TMTARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFH
                     FPLIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMED
                     KRGEEHIKLSTEYGGKTQLNLGHNVNASRELRGEGAELRTNDWVSVRGGKGILLTADT
                     QPDVGSKMLEMDAAIEQLTQALTLARNLQAAVKGAKATPSDIDGQKKLNSALGYLKMP
                     GLLAHAPSGIGILSPQTVRLASGEQSIGLMSGRNTDISTGESFTVAATDTISLFARDA
                     GMQLFAGDGKVDIQAQGDELNLSSLRDMTVSSTENSISINAAKELILNCNGAYIKLSG
                     GNIELGCPGNILLKATNVDQTGPASLNTPPVSFPEGYGETFITTSRQSGKIKPFTTYR
                     ITTSEGEVYEGISDSEGKTSSIFTSMPSQIKIEYPRNVSENKEEE"
     gene            17542..19563
                     /locus_tag="ACHKUB_002983"
     CDS             17542..19563
                     /locus_tag="ACHKUB_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HFO6655966.1"
                     /translation="MTTAITSLKHKYPHAPAEEQLPYWVEILLVDEQGDAVSDMPWKV
                     ESHHPGDGIIKQFTYTGRSGSDGLIRIDMPHGLELKLTLDADSLAKEMEKRPLRVGRD
                     AEMDSLIRQLAEDKGYIWHYAVIGELCKVTPSIKLESGEGWPPYHFPHGKSFKGFIIR
                     TNELERRHVIEICPFRAWELVLHHQKDYSIANGINLGVAATLAYADDNALSKSSITNF
                     FINQCQDLSRLPRLYKDKSSWNTLVRDVPYSERYSPPVFMDTSIDSSSKEDKDNQQGT
                     ISTKNDVSNVKADGDTQLYYVYNSDKIIIAWRGTESLFDAGTDIAFNPVKTESCDVNK
                     TQCAALVPAGKVHNGFWSGYLRLGRVFNNELDELLTLIKSRDLFVCGHSLGGALALIH
                     SAALKLEKPLLYTYGMPRTFTRNAIMELSDITHFRHINDNDPIPAVPMEANLDNEWYK
                     LWGLLGGTLGFFWSLGELMAYQLVAWGDCFWHHGNTVAFLTVTQSREWKEYKRNLPSP
                     AGGITIRKRLPVKAKLYLVPVLAEQEMQEAGQKQKEFQASLTKTDLTRFFPQGGNPER
                     GVNINIFEHFMTSYMPYMYNKLLELIDKAGIVEKRTFTEHLYNIDLFKMQMEENKGCI
                     PDKEFSRNKIFLDIENMLDVSLSSTLSMPSGNDTLLRFAKYSEEVMENA"
     gene            19556..>20133
                     /locus_tag="ACHKUB_002984"
     CDS             19556..>20133
                     /locus_tag="ACHKUB_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6655967.1"
                     /translation="MHRSSNLLFLSLMLFSVTGCSFNQLPGTIISRSPDKKLSPPGDT
                     KWVNIEIKNPSPYTKPFPLSVRYISYECQKKRISGFDGSVVSEPGYNVKEIPMQQERG
                     DIWKAKVAMTGGGSCNWTLSAVTLGIEYTDATHLGKGLVPGSAVGVRLAFDGYASRNG
                     QFDFIGGNQFDYYPKYYPFIKKWSDSSQGLMH"
BASE COUNT         4856 a         4707 c         5620 g         4950 t
ORIGIN      
        1 tgaacagagt gcattaagca tccgcctgag cggtaaacgt atccgatctg gtgagtgaca
       61 gtctgagagc attctcagta atcgccccct tgtatcctgg aaattggtta agagcgatta
      121 gatttggcaa ggtgtatgca tccagcagct taacacctca gctaactggc tggttacctt
      181 caggttgtcg caatggcatc ggacatccgg tgtcggataa ctaaacgtag cgttaaggcg
      241 atattgctga gagaccggtt aagtggtggc acgggagtaa tgagtttggg gagggtacca
      301 gaatgccagt caggtgcggt atgctggcag tatcacgttg attgcagcat aaagtaacga
      361 gagacgaatg gaagttaaaa ccagagcagt tccgtcgttg ttcatcccgg gagacgctgg
      421 aaaaagtgat tacccgcaca cgttataaac ttacccctgc ggagctggaa gcctttaact
      481 ctgcggtcga tcaccggctg gcagaactga caatgaacaa actttacgat cgcgtgccgg
      541 cttccgtctg gaaatatgtc acctgaatct tgaatccgcg ctacgatgac tctggtgtta
      601 tctgtgcacc ggaagatcgg gaacattaag cagtataacg gcaacaaaga attcgaaatc
      661 aaaagggaat ggtgtacaga ggggcaggcg ttcaggcatt accggcctgt atcttctgaa
      721 ggggactcgt atttgtcggg gctgagtatt ccgctcgatt cgatcacgga ttggcagaaa
      781 cctgactgtg attgattcag gtaccatttt gcagggatcc ggtgcgttac tgtctggcac
      841 aacaaacgca gcgggtcgct ctgccccgcc gattcctgat aattcctccc ctgtaaccta
      901 ctgcaacagt acaaatacac tgacgcctga aacggtaacc agaatcagca ccagcagtaa
      961 cgtcagtaaa cagagcagac gatcggtctt ccggtaataa ccgaaggggt cgacagcgcc
     1021 acaatacggg caacacactg tattatgagt aattgcgttt ccacagtccc tgcacttctt
     1081 tttgcgtatc acgatcgctc cctctccacg ccttatccgt aacctgtttt ttacattaaa
     1141 aaaacaacgc cgggaattca atcgtcagtc cggagacgac cgttcgggtt atcacagagt
     1201 gcctgagaca gtgtcctgcc ggaggtcacc tcaggggctc tgcgcatttt ttacgacgtg
     1261 gctattccgt atgaaccata cggagattta accatgacct acaaatacaa ccccttctgg
     1321 cagcaacgta ttcgtgagac ggtgcggcac gcactggatg ttcatccccg cctgacggca
     1381 cttcgggttg atctgcgtct cccggatgtg ccggcagcaa cggacgcggc tgtgatatcc
     1441 cgcttcatca atgccctgaa agcccgaatc gacgcttatc agaaacgtaa gcatcgggaa
     1501 ggtaaacgcg tgcattcctc gaccctgcat tacgcctggg cccgggagtt cggggagctt
     1561 aaaggcaaaa aacactatca cctgctgctg ctggtcaacc gggatacctg gtgtcgtgcc
     1621 ggcgattacc gcgctccggg gtcactggcc gggatgatta aacaggcctg gtgcagtgcc
     1681 ctgggagtgg atgccggacg ttatgacacg ctggcacatt ttcctgacaa gccagcggtg
     1741 tggctggaac gcgatgatga caccggcttt cagcaggtgc tggagcgtgc tgactatctg
     1801 gcgaaggaga gtaccaaagc ttacggcacc ggtgaacgca actttggctg cagtcgtggc
     1861 tgagcccgac agacgactta tctgacctta gtgtgatgac gtgttcacag cgaccacact
     1921 gattttccct tctgagactg aactgaaaac cgccattcac acctgaccac gctgcccgta
     1981 ccgggacggc tgttgcctgc gtgtcgtctg gcggctgagg aacatatact atgtacgcaa
     2041 aatcctttct cgctcttgat ggcaacggac gtctgacggg ggcccgtacc gcacagactg
     2101 cgccttacga ccgctacacc tgccaccttt gcggcagtgc actcagatac cacccgcaat
     2161 acgacactga acgtccctgg tttgaacaca ctgacgacgg gctgacggag cacggtcatg
     2221 agtgccctta tgtcagaccg gagcgcaggg agatacaact gattaaacgt ctgcagcaat
     2281 tcgtaccgga tgccttaccc gtagtacgta aagccagctg gtactgcaga caatgtcacc
     2341 acgattatta tggggagcgg tactgcacac actgccagac cgggcgcttc agtgaggagg
     2401 gggtagcaga atgaacggta taaaaacggg gctggggatc acgccgggtg agcacattat
     2461 cagtgccgac tctgcgctca gcagaaatat ccggcattgt ttctgccttt cctgccatgg
     2521 ccggttgatt ctgcagacag atgctcaggg ggcatggttt gagcatgatt tacatgcctt
     2581 gagtgagcaa cagaaggcgg actgtgtggt gctgaatccg gaaaagtccc acccttacat
     2641 tgaggatatg gcgatgtttt tatcgccatt accggtcgtg ctggagtggc attgtgtgat
     2701 gtgtgagagg ctttttcatg ggaagaagtt ctgtgaggcc tgtggtacgg ggatttattg
     2761 cagagcggta tgtacaaagt ctgtttacag gtaccagcca gatttgctcc ggaacccgga
     2821 gtgaggagga tgcaacataa ttgtttaatc acgccagaca cgcccctgcc ggatccggat
     2881 gtggtccggc gttttgttct gcggacggtg gttcaatggc tgtgtctgct gatgctcctc
     2941 tttatgcgac attcagacag gggagacatc gataaacatc atctgcgggc aaatagttac
     3001 acagaatgat cattgctacc ggttatggca aaaattggtg atgaccaggt gaaccatcgg
     3061 tgctttcgtt gattcttatg gactgataaa gttttgtcag ctgaatcttt atacggaatt
     3121 cttttcagta tgcgtacacg aattgactat cttgcggata aatacagttt tactgaactg
     3181 aatgaacttc cacgccttca ccagcagtgg caggatgttc tggaggagtg ccggcagaca
     3241 gaggccgggc cggaagaacg gctgcgtatt gcactgctga atgtggatta cgtcaccagt
     3301 tttgaactgc cttttcgctt gttgcttacc cggacaccac aactgattgc cgcgctgcgt
     3361 gaagaatggg gcatcagcca gaaaaatgtg gtgttcaatg ataaacgatt tggctgcgtg
     3421 tacagcctga aggccagcct ttccggtgtg ccggatacat accggtatca tctgtctcat
     3481 cgtattcgcc gggtgattgg gaatgaaaac acatcatcgc cataccagca gattgcccgg
     3541 gaagtgaaag cgccccgtga acggctgaag catgcgctgg aggcggggtt gctggtgact
     3601 gcactggacg gcctgttctg gttcggcagt cagcgtattg cggctgatgt cctgaggctg
     3661 agaaagagcg gaatgccggt ggtgacaacg accgcggagg tgcatgataa tctgacggga
     3721 acaacccgca aagtaccggc ctaccatctc tgattcgacc atcaggggga atattgctct
     3781 ggcagcagca gtacgacatt ctttatcccc gagcctgaaa acatgtgcta aggaaacggg
     3841 aatggcaagg tccgattacg atattatcaa tctgtctccg gaacatgaac tgaatgagtg
     3901 gctggcagag agaggttatg ccggacaggc ggataaccgt aaccgggtgg cagaaattgt
     3961 tacccgcaaa ttgcaggaca gcttttatat caacgtttcc tgggatgcgc tgaacacggc
     4021 atacagtgaa cacccggagt ggttttctgg gcttgtctcc ggggatgaga attaataagt
     4081 ggattatgct gccatcaggc agtgtgattg ctggcagggg gggctttccg gggttcgggt
     4141 ttgaattttc ccaggaagtt gtacatcggc cgcatgcagg gatgcggata gccgtcccgc
     4201 ataaacacca cccgggtttc ctcaacactg gtcacggtga caagcgcgcc atgatcgtct
     4261 ctgtatcgct gattgatttt tggtaatgga atatcttctg acatagactt cagaacggta
     4321 accgttcact aaattttcat gtggtatcac tgaatcagga gacgacgttt cctgatgtgc
     4381 gttgttaatt ttctgttttt aaaaatattt tctctctctt ctttattttc cgtgtctttt
     4441 attgctctcc tctttcatgt ccttctcttt ttctgatatg cccttaaatg ctgccgaatc
     4501 atcactcctc gtgaatgtca gtgctggtaa tcattcggta taaccatctg tatcaaaagc
     4561 actaaccgga ataattttct gcatttttgt attacccctg gatataagtt ttgataaatg
     4621 ctgattttgc gatatgtatc agaataaaga tctggttatt ggcattaaaa cgatcgatat
     4681 gtctgtcata ttgagaattt gcagatcttc gtcctataat gggtgaaatt aaatcagtgc
     4741 acaaggggag gccttctgtg gcgatgggct ccctgggatg caggtttcac aggagaacgt
     4801 catgagcagt tcgtttcaga atgagatccc gaaggcaagg gtgaatctga aattagacct
     4861 acatacaggt ggtgggcaga agaaagtgga gcttccgctc aaactgctgg ttgccggtga
     4921 tttcagtaac ggacaggaag agcgcccgct gtcagagcgc ataaaaatgg atgttaataa
     4981 aaataacttt aatgctgttc tggctgatat ctcgccgtca gtacgcctga atgtggagaa
     5041 cacacttgcc ggtgatggta ctgaagagag catcagcctg actttccgta atatgaacga
     5101 tttcacgccg gagcaggttg cccgacaaat tccccgtctt aaagccatgc tggccatgcg
     5161 aagtttgctg cgggatctga aggcgaatct gctggataac gccactttcc gtaaagaact
     5221 ggaaaaaatt ctgcgggatc ccgaactgag tcaggcctta cgtgacgaac tgagtgcact
     5281 ggtgccggaa caggtgtgat taacggatta agagagagca tgctgatgtc tgtacagaaa
     5341 gaaaataatg tggcgggaaa tgcggtttct gaaacgcatt ccggcgacag cgtatatgct
     5401 tccctgtttg aaaaaattaa tctgaatccg gtatcagccc tgagtgcgct ggatatctgg
     5461 caggatccgc aggccatgtc agaggcatca gcagatgaac gtctgaccgc agggatgcag
     5521 gtgtttatgg aatgcctggc gaaagcggga gcaccggtgg agaaactcga taaggcgctg
     5581 attgaccatc atattgcaga gcttgattat cagatcagcc gtcagctgga tgccgtgctt
     5641 catcatccgg aatttcagaa agtggagtcc ctgtggcggg gcgtgaagtc tctggtggat
     5701 aaaacggact tccgccgtaa tgtgaaaatt gaactgctgg atctgtccaa agacgatctg
     5761 cgtcaggatt ttgaggatgc gccggaaatt attcagagtg gtctttatct gcagacttat
     5821 gtggcggagt acgatacgcc gggtggtgaa cccattgcag ccctgatttc tgcctgggag
     5881 tttgatgcgt ctgcgcagga tgtggcgctg ctgaaaaata tttcccgggt ggctgcgtcc
     5941 gcgcatatgc catttattgg ttcagttggc ccggctttct tccggaagga tacgatggaa
     6001 gaggtggccg ccattaagga tattggcaat cactttgagc gtgccgagta catcaagtgg
     6061 aatgctttcc gtgaaacgga tgatgcccgc tatattggtc tggtgatgcc acgtgttctg
     6121 ggacgcctgc cgtatggtcc tgataccgta cctgtccgca gctttaacta tgttgaagag
     6181 gtgaaaggtc cggatcatca taagtatctc tggacaaatg cgtcttttgc ttttgcggcc
     6241 aatatggtgc gcagttttgt caccaacggc tggtgtgttc agatccgcgg tcctcaggcc
     6301 ggcggtgcgg tgcaggattt gccgatccat ctgtatgatc tgggaaccgg taatcaggtc
     6361 aaaattccgt cagaggtgat gattccggaa actcgggagt ttgaattcgc taatctgggc
     6421 tttattccgc tctcttacta caagaaccgg gattatgcct gcttcttctc cgctaactcc
     6481 acccagaaac cggcactgta cgatacgccg gatgcaacgg cgaacagccg tattaatgcc
     6541 cgtcttccct atatcttcct gctgtcccgc attgcgcatt acctgaagat cattcagagg
     6601 gagaatatcg gtaccaccaa agatcgccgt ctgcttgaac tggaactgaa taactggatc
     6661 cggggtctgg tgacggagat gaccgatccg ggtgatgaac tgcaggcctc acatccgctg
     6721 cgtgacggaa aagtggtggt ggaagacatc gaagacaacc cgggattttt ccgcgtgaaa
     6781 ctctatgctg taccacattt ccaggtggaa ggaatggacg tcagtctgtc actggtttcg
     6841 cagatgccaa aggcaaaagc gtaaggcagg cgtcgggaaa tgaagattta tcgtccactg
     6901 tgggaagacg gggcttttct gatgccccag cagtttcagc agcaggctgc ctgggatgtt
     6961 catctggcag acagcgttgc cagaatgggg ctggcacatc cctggggcgt tgtggccgca
     7021 gagtttgatg actctctgtt acctctttca cggttgaatg ccacccgtct gattgttcgt
     7081 ttcccggacg gaacgcttat tgatacggaa cgtgcggata atctgcctcc ggtctgtgat
     7141 ttatcgacgg tttctgatcg ttccctggtg gatattgtgc tggcgctacc gttactgaat
     7201 gctaacggtg gcaaccttga tgatggccgt gagagcgagc gtccgcgtcg ctggaaatca
     7261 gaaagggtga atgtgcagga gctggccggg catgagcaaa gcgaagtggc ggtattgcgg
     7321 cataacctga ctctgcgcat ggctcatcag gaaaatgctg cctggctgac ctgtcccgtt
     7381 acccgtcttg ttcgtgatgc acagggacag tggtgcaggg acccgcgttt tattccccct
     7441 ctgctgacgc tgtctgccag tccttcgctg atgactgagc ttgcggagtt gcttcatcac
     7501 ctgcaggcgc gtcgccagcg actgatgtcc atgcgtcggg agaacaatgc ccgtcttgcg
     7561 gattttgcag tggcggacgt gtcgttgttc tggttgctga atgccctgaa cagtgcggag
     7621 cctgttctga aagagttgct tgacatgcct taccgccacc cggaactgtt gtacagggag
     7681 cttgcccggc tggcaggctc cctgctcaca ttctcgcttg agcataatgt ggatgcggtt
     7741 ccggcatacc gtcatgaaac acctgaaaat gttttcccac ctttgctttc tttgctgaac
     7801 cagctgctgg aggcaagcct gccttcccgt gtggttttca ttgaactgaa acaaaatggt
     7861 gtgatgtggg agggggcatt acacgatgcg cgtctgcgcg aaggggcgga tttctggctt
     7921 tcagtgcgct cttccatgcc cgggcatgaa ctgcagacga agtttccgca gttatgcaaa
     7981 gccgggagcc ccgatgatgt gtctgaggtg gtgaatgtgg cgctgagcgg ggtcattatc
     8041 cgtccggtta cgcatgtacc ggcggccatt ccgctccggc tggaaaacca gtatttcgcg
     8101 ctggatttaa gcacggatgc ggcccgggcg atgctggacg cggggcgttg tactttctac
     8161 accccggcat cgctggggga tgtgaaactg gaactttttg cggtactgcg gacatgaata
     8221 aacctgttat ctcccgggct gaacagattt tttatcccgg ctggctgatg gtctgccagc
     8281 tgcgcagcgg acaaccggtt gaggatggta aggccctgta tcgccgtgcc tgtcagctgg
     8341 tgaaacaggc gcgtgaagag ctggccgaag caggattcag tcaggaaaac agtgaaatca
     8401 tgttgtatgc cttctgcgcc ctgctggacg agagtgtgct gaaccgtgaa aaaacagacg
     8461 atggctggca cacctggcag caggacccgc tgcaggctca tttttttggt acgctcaatg
     8521 ccggtgaaga gctctgggag cgtattcgtg agcaactgaa attaccagcg ccggatgtgg
     8581 cggtactgac atgcctctgc cgtacgcttc agctcggttt taccggtcag taccggtcgc
     8641 aggatgatga gcgtcgcgaa gatgtaatac gcgcactgac agcgcgtgtt cctgcattta
     8701 ctttcgcaca ggatgcgccg gtggtggtgc gtgcaccggg gtaccgtgct ggcagaacga
     8761 tgtactggtt gtcatgggtg gcgggtatta tgacgctggc tggtctctgg tgtgtgcttt
     8821 cttctgtgct cgcagaccag gtggcacggc ttaccgggca gggataacca tgcacgtacg
     8881 ttcccgtctc tggctgacaa tactgagtct gttcctgaca ctgtggctga tttcgggatt
     8941 cctgccactg tctgcagtaa accagataat actgaccctg gtttcagtcg tggtggcggg
     9001 gcttgtgctg tggtatcagt ggcgttgtct gtcttcccgt cgtgatatcc cggagaatgc
     9061 cggcgatgac ggtctgccgc ctgagtattt tcaggggcat gtgctgcttg ttgccggaaa
     9121 ctcagatgcg tggttcgaac ccggtcaggc ataccgggaa acagcatcgg gatggtatct
     9181 tcgtgtggat acgccggagc agatgagaat actggcagag cggttgtcgt acagccgtcc
     9241 ggccctgtgt ggccaggtcg tggtcatgct gggactttta ccggagcagc atacctcaga
     9301 tgaagctttc ctgtcgtggt tgcggaactg gcagcgtgca gtcatgcagt gccggcagtg
     9361 gctgaatacc atgccgccgg tgctggtgac attgatggtt tcagggccgt caccacaaaa
     9421 cacatcctta aataagcact ctgcacactg gtttacactg acaccggacc tgtcgggaat
     9481 gcacatacgc ctgacggaag ccggagccat tccggcagag gaatggctgc tggcgtgtga
     9541 tgcagcgtcc aggctccggt gcgtcagtga actgttatgg ctgaatgcca tgttgccctg
     9601 gtacaaccgg gttactgatg ccctgacaga tatttgccgg caggagcacc tttatttccg
     9661 gccggttgcc gtcggattct gtctgacgtc ggttgctgcc cgacccggta atatctggca
     9721 acaacaactg acgtccctca ctgcactgcc accgtctgcc ggcgtgtcat cacaggtact
     9781 gccgttgccg gatattctgc tgtccggtat cccccgtcat cgaggactca gtcactggca
     9841 cagactttgt cgccagacgg ggttgattgt cggtgttttt cttctgctgg ctatgctggc
     9901 gtcttttatg aataaccagc gactggttcg cagtgtgggg gaacacctga cggcctggca
     9961 ccggatgcgc ggatattcat ctgaattgaa aacaactgca ctgcaacgtc ttcaggctga
    10021 cagccagtta ctgaacgact ggcagcaaca tggtgtgccg cagcgttacg gactgggatt
    10081 gtatcaggga atgtggctga taccgccggt tgagcgagcg actggtgagt gggttgcacc
    10141 actgcctccc cgtccggtga tacagaaagt tactcagacg ccgaaaacgg ttcgccttaa
    10201 cagcctggcg ctgtttgatg ccggtaaatg gacgctgaaa ccgggcgcca caaaatggct
    10261 ggtgaatgcc ctggtggata ttaaggcaaa agcgggctgg ctgattgtgg tcagtggtca
    10321 cacggacaat acgggtgacc cgcagcgcaa ccaggccctg tcactgaagc gcgcagaggc
    10381 cgttcgcgac tggatgcggg atacgggcga tatccctcaa agctgttttg ccgtacaggg
    10441 atatggtgag agtcgtcccg tggctccgaa tgagacggcg gaagggcgag cgcgtaaccg
    10501 ccgtgttgaa atcagtctgg taccgcaggc cgatgcctgt cgtctgccgg acatcacccg
    10561 ggcgccgtaa cagggcgcgt aagacaacga gagggagtaa ctttatggca attccagttt
    10621 atctgtggct gaaagatgat ggcggtgcag atatcaaagg gtccgtggat gttcaggacc
    10681 gtgagggcag cattgaggtg gtggctcagg agcactgcct ttacattccg acggataaca
    10741 ataccggtaa gctgaccggt acccgtattc acacgccgtt cctgtttacc aaggaaattg
    10801 attcctccag cccctacctt tacaaggcgg tgaccacggg gcagaccctg aagtctgctg
    10861 aatttaagtg gtacagaatc aatgatgccg gtcaggaagt ggagtatttc aatacgaagc
    10921 ttgagaacgt gaaggtggtg aaggttaacc cggtcatgca cgacatcaag aatcccgcct
    10981 acgagaagca taaccacctt gagcaggtgg agctgcgcta cgagaaaatc acctggactt
    11041 ataaggacgg taacatcatt cattctgacg cgtggaatga acgtaccact gcgtaattct
    11101 cccggacggg cagggtatcc tgtccgtcat ttttcttttt tcttcagtat ctgttggcgg
    11161 gtgctgaaga aagaattctc aatcctgaac acagacctga tgcgcccggg ttatggcgaa
    11221 gggcggtagc gtgtctgaag gtcttctgac agtgagtgaa taacatggaa aatccggcag
    11281 ctctgttacg ccgtcttaat cattactgtg cccgtgcact ggaaggcgca gcctcccttt
    11341 gccagacccg ggcccatgcg gaaatcaccc cggagcactg gttactgaaa ctgctggagc
    11401 agggcgaagg agaccttacc gtgctgggcc ggcgttatga ctgggatatg gacgcgatat
    11461 ggcagtcgct gctcggctgg ctggataacc agccccgtag cgtacgcagg cgcccgcagc
    11521 ttgcgccgtc cctgaatgcc ctgctgaaac aggcctggat ggtggcctca ttacagggag
    11581 aagaacatat ccgcagcgtg catctgctgg gtgccctgac ggaaaatccg cacctggttc
    11641 gctgtgacgg gctgtggccg ctgctgacac tgagtcatag ccagttgcag cgtctgtccc
    11701 cgctgctgga tgcgcagtct gatgagtgtc cggagagatt acaggatgca gagcctgtgc
    11761 tgcctcaggg agacaatgtg acctttatcg ggcgtcccgt cggtgcggat acggcgggta
    11821 taccgtcagg tgacctgccg cctgtgttac agagtgctct ggataaattc acccgggaca
    11881 tcacggccag cgcgagagag gggaaaattg acccggtatc gggacgcgat acggaaatcc
    11941 gtcagatggt ggatattctc tcccgccgcc gcaagaacaa cccgattctg gtgggggacc
    12001 ctggtgtggg gaaaacggct ctggtggaag ggctcgccct gcgtattgcg gaaggtaacg
    12061 taccggaatc actcagaccc gtcaccctgc gcacccttga cctcggcctg ctgcaggccg
    12121 gtgccggcgt gaagggtgaa tttgaacagc gcctgaaaaa cgtgattgat gccgtgcagc
    12181 agtcaccggc tcctgtactg ttgtttatag atgaagccca tacccttatc ggggccggta
    12241 atcaggccgg cggcgcggat gcggccaacc tgctgaagcc tgcgctggcg cggggtgaac
    12301 tgcgcaccat tgccgccacc acctggtctg agtacagaca atacctggaa cgtgacgcag
    12361 ctctggagcg gcgttttcag atggtcagag tggacgagcc ggatgatgag acggcctgtc
    12421 tgatgctgcg ctccctgaaa tcccgttatg cggaacatca taacgtgcat atcaccgatg
    12481 aggcggtacg tgctgccgtc acgctgtcgc gccgttatct gacagaacgt cagttaccgg
    12541 acaaggccgt tgacctgctt gataccgctg ccgcccgtgt gcggatgagc ctcgatacgg
    12601 tgccggaaca gctgacccgt atccgttcac agctcgcttc cctcggtatg gaaaaacagg
    12661 cactgctgga agatattgcc gttggtcatc agaatcacgg cgaacgcctg tctgccattg
    12721 agcaggaaga gaacgtgctg atgacagcac gtgatgacct ggagcagcag tacgcccggg
    12781 agcgcgaact cacaggcgag ttacttgaaa gccgcagcga tatttcccgt cagagtgaga
    12841 cactgcatct gcagcaggca ctgcacgaca ttcagcagaa tcagcccctg ctcagtgtgg
    12901 atgttgacgt acgcaccgtt gccggtgtgg ttgcggactg gaccggggtg ccgttatctt
    12961 ccctgatgaa ggacgaacag acggaactgc tgcatctgga aaaggatatc ggcagacggg
    13021 tggtcggaca ggacgtggca ctgggatcca ttgcgcagcg tctgcgtgcg gcgaaaacgg
    13081 gtctgacatc cggtaacggc ccccaggggg tgttcctgct ggtcggtccc agtggtgtgg
    13141 gaaaaaccga aacggcactg gcgctggcag acgtgatgta cggcggcgaa aaatcactga
    13201 ttaccatcaa tctgtcggaa taccaggagc cccatacggt ttcccagctt aaggggtcac
    13261 cgcccgggta cgtgggatac ggtcagggcg gtattctgac ggaggctgtg cgtaagcgtc
    13321 cgtacagtgt ggtgctgctg gatgaagtgg aaaaggccca ccgggatgtg ctgaatctgt
    13381 tctaccaggt gtttgaccgg ggctttatgc gcgacggtga ggggcgtgaa attgacttcc
    13441 gtaatacggt cattctgatg acctccaatc tgggcagtga ccttctgatg cagcaactga
    13501 gcgaaaagcc ggagacaacg gaatcggagc tgcatgagct tatccgtcca cggctgcgcg
    13561 accacttcca gccagcactg cttgcccgtt tccggaccgt gatttaccgt ccgctgacgc
    13621 agtctgctat gcgcaccatt gtggagatga agcttgcaca ggtctgcgaa cgcctgcact
    13681 gccattatgg gctgagcaca tcggttgatg aacgtgtgta cgatgccctg acatccgcct
    13741 gcctgctgcc ggatacgggc gcccggaatg tggagagtct gctgaaccag caactgctgc
    13801 cggtactgag ccggcagttg ctgagccata tggccgcgaa gcagaaacca caggcgctgg
    13861 ctctggcatg gagtgacgaa gacggtatgg tgattgagct gcggcaggaa tgcgcgttat
    13921 gacaggctat ggcagaaatc aggacgctgg cgcaggtaaa aatcaaaggg aagaaggctc
    13981 attgcgatta ctgacgccgg gagagattgc gctggcgagg ctggtattca ggtcgacaat
    14041 cccttatgaa aaggtctgga tccaccatga cagttttctg ccttttggtc ttcagaataa
    14101 gcaaactgcc atggtccccg atggtaagat ttatttccgt gagcactatc gggatgatta
    14161 ttcactcagc gttccctttt atcagcatat gtttattcat gaaatggcgc atgtctggca
    14221 gcgtgaaaag ggaatgaatg tcatcggtcg ggggatgttc agttgggcgg tgaattaccg
    14281 ttatgttctg gacgggcgtc tgttaagcga atatcccatg gagcagcagg cacaaattat
    14341 tgctgatcat tttactttgc aggctgaagg atacgggaca tggtgtgata tgagaaggga
    14401 cggtgatatc acactggacg gaaatatgtc tgagtatgtt attcgcagcc tgtataccag
    14461 cacgttgcgg gggttcccat ggtaaaaata agagcctttc gttatcgttc tgctttactg
    14521 ctctgtctga cgttactgac aggatgcccc ggacgcgggg attattatcg gtttgatgaa
    14581 acagtaacgg tcagcacaag aggcgacaat gtgtgtttca gtgtcccgga gccagaggac
    14641 taccagccag tcagcgtttc catcgctcca cggggaaccc gattcaggga gcggcaaatt
    14701 acatctgaac cctcgcttcg tatcgtgaac ggccagctgt gcattccgcc ttcattctgt
    14761 ctttttcctg ataaaggcca gttcattgtc aggtctgttc tgtactctga gcgcgataag
    14821 gacacaccgc gcatcatggt tgccggcata gagattgccg acgggtgtat tttcgatatc
    14881 ccgcttaatg acatggaggt cgtgaggcca tacggtgagc tgaaaagcag taatgttcag
    14941 actgaccaga gcgaacgtca tggtccctgc aaataccctt tcagagctgt tcgtggagtg
    15001 ataaatcatg aatctcactg actccctgca aaatgtttta tccggggtgg gaaccctgaa
    15061 ccgctacagg ctggatatac cttcctgccc gtcatcgctg gatgttgaag atttcagcgg
    15121 aactgaaggc ataagtaaga tttaccggta tgacatcatt tttaccagta cggacaggta
    15181 tcctgatgct gcctggttcc tgcgtaaatc cgcaaccctg ataatgggaa ccggattact
    15241 ggaaagcctg actgaacaga aaaaagtgca cggtgttatt actgatttca gacgtatttc
    15301 tggctctgag gaccaggcac aataccggat cacacttaaa cctttcattt ccctgctgga
    15361 taaacagttc cgtacacacc gttttttcgt taataaatca gtgcctgagg tggtggagca
    15421 aatcctgacg gaacacgggc tgaaaggctg ggagtatgag ttcagcctca aaaggacgta
    15481 tccgaagcgc gaacagatta accagtatca ggaaagcgat ctgagattta tccagcgcct
    15541 gctggcagaa gtgggcattt tttacttctt tacgttgcac ccggatgcgc agacagaggt
    15601 gatccacttc ggtgacgccc aggcggcgtt gatatttgat aaaacactgc ccgttaacag
    15661 cccgtccggt atgagtgaca gtggcacaga ctcagtatgg gcattgaatg tggagcaccg
    15721 ggtggtggaa agccgtgtta tcacgaacga ctataaccac cgcgaggccc agaacatact
    15781 gatgtccgta ccggcggaca tgacccgtgg agaaggttac gacaccagtt atggcgaggt
    15841 ttatcattac cggccacgcc atacagaaag gggcgataaa attgaccctg cgccggagac
    15901 ggctaacttc tgggcccggc tcgatcacga gcgttttctg gctgagcaaa cacgtatcac
    15961 aggtaaaagc accgatgcca gtctgttacc ggcacaggtg ctgactatta ctgacagtac
    16021 tccaccagtg ttgcctgcac cgttacagga gccggttctg ctcacgcagc ttctgttcac
    16081 cggtagccgg aaatctgcgc tacaggtgat gctgagtgct gttccttaca gcgagattgt
    16141 ctgctggcga ccaccgttac ttactcgtcc gaaaatcacc gggaccatga cggcccgtgt
    16201 gaccagtgca aaagaagggg atatctacgc ctggcaggat gcttcaggca tgtaccgggt
    16261 gaaatttgat gctgaccgtg atgataaaaa tccgggacag gaaagtatgc cggtacgtct
    16321 ggccaaaccc tacagtggtg atgcttatgg ttttcatttt ccgctgattc agggaacgga
    16381 agtggccatt gcttttgagg aaggtgatcc ggatcgtccc tatatagcgc atgccctgca
    16441 tgattcacgt catgttgatc acgttaccga taagaacggt acgcgcaatg ttatccgtac
    16501 gccggcaaat aacaagttgc ggatggagga caaacgcggt gaggagcata tcaagctcag
    16561 taccgagtat ggcggtaaga cacaacttaa tcttggccat aacgttaatg cctcccggga
    16621 acttcggggg gaaggtgcag agctgcgtac aaacgactgg gtcagcgtgc ggggagggaa
    16681 ggggatatta ctgaccgcag atactcagcc tgatgtcgga agtaaaatgc tggaaatgga
    16741 tgcggcaatt gagcaattga cccaagctct gacacttgcc cgaaatttgc aggcggcagt
    16801 aaaaggagca aaagctacgc catcggatat tgacggtcag aagaagctta atagtgcgct
    16861 gggatacctg aaaatgccag gattactggc acatgcacct tctggaatag gtattcttag
    16921 cccacagacc gttcgtctgg cgtcaggaga acaaagtatc ggtctgatgt ccggaagaaa
    16981 tacagatatc agtacaggcg aatccttcac tgttgcggct acagatacga taagtttatt
    17041 tgcccgtgat gctggtatgc agctattcgc gggggacggt aaagtagata tacaggctca
    17101 gggggatgag ctcaatcttt catccctcag ggatatgacg gtcagcagta ccgagaacag
    17161 tatttcaatt aatgctgcta aagaacttat attgaattgt aatggtgcat atatcaaatt
    17221 gagtggaggc aatattgaac tgggatgtcc cggtaacatt ctgctcaagg ctacaaatgt
    17281 agatcaaaca ggtcctgcat ctttaaatac gcctccggtt tcatttcctg aaggatatgg
    17341 agaaacgttc attacgactt cacgtcagag tggaaaaatt aaacctttta caacatacag
    17401 gatcacaaca agtgaagggg aagtgtatga gggtatttct gattcagaag gtaaaacatc
    17461 atcaattttt acatcaatgc cctctcagat aaagattgaa tacccgcgta atgtatcaga
    17521 gaacaaggaa gaagaataat catgactact gcaattactt ctcttaaaca caaatatcct
    17581 catgctccgg cagaagaaca attaccctat tgggttgaaa tactgcttgt ggatgaacaa
    17641 ggggatgctg tatcagacat gccatggaaa gtggagagtc accaccctgg tgatggaata
    17701 attaagcagt ttacatatac cgggcgaagc ggttcggatg gtcttataag aattgatatg
    17761 ccacatggtc ttgagttgaa acttacactg gacgctgatt cactggcaaa agagatggag
    17821 aaacgccctc ttcgggttgg cagggatgca gaaatggatt cactcattcg gcaattagca
    17881 gaagataaag gatatatctg gcattatgct gttattggtg agctttgtaa ggttactcca
    17941 tcaattaagc tcgaaagtgg ggagggctgg cctccttatc attttcctca cggcaagtca
    18001 tttaagggat ttattatcag aacaaatgag cttgaaaggc gacatgttat tgaaatatgc
    18061 cctttcaggg catgggaact tgttctgcac catcaaaaag attattccat tgcaaatggt
    18121 attaatcttg gcgttgcagc aacactcgct tatgcggatg ataatgcttt aagtaaatct
    18181 tcaattacga atttttttat taaccagtgt caggatcttt cccgtctgcc tcgcctttat
    18241 aaagataagt catcctggaa tacgctggtc cgggatgtgc cttatagtga acgttattca
    18301 ccacccgtat ttatggatac gtcaatagat tcctcatcta aagaggataa agataatcag
    18361 cagggaacta tttctactaa gaatgatgtt tcaaatgtaa aggcggatgg tgatacgcag
    18421 ctttattatg tttataattc agataagata attattgctt ggcgaggtac agaaagtttg
    18481 tttgatgcgg gtacagacat tgcattcaat ccagtaaaaa cagagtcatg tgatgtaaat
    18541 aaaacacaat gtgcagcatt agtgcctgca ggaaaagtgc ataatgggtt ttggagtgga
    18601 tatctcagac ttggaagagt atttaataat gaacttgatg agcttttaac gttaattaaa
    18661 tcaagggatt tgtttgtttg tggacatagt ttagggggag ctctggcatt aattcattct
    18721 gcagcattaa aattagagaa acctctgctt tatacttacg gaatgcctcg gacctttact
    18781 cgaaatgcca taatggagtt atcggatatt acacattttc gacatattaa tgataatgat
    18841 ccgattccag cagttcccat ggaagctaat ctggataatg agtggtacaa actttgggga
    18901 ttgcttggtg gtaccctggg tttcttttgg tcattgggtg aactcatggc ttatcagttg
    18961 gtggcctggg gagactgttt ttggcatcac ggtaatacag tagcttttct tacggtcact
    19021 cagagtcggg aatggaaaga atataaaaga aacctgccaa gccctgcagg ggggattact
    19081 atcagaaaac gactccccgt taaagctaag ctttacttag tacctgtatt ggcagaacag
    19141 gaaatgcagg aggcaggtca gaaacagaaa gaatttcagg cgtcactaac aaaaacagat
    19201 ttgactcgtt tttttccaca aggtggtaat cctgagcgag gagtgaacat aaatattttt
    19261 gagcacttta tgacgtcata tatgccttat atgtacaata agttgcttga attaattgat
    19321 aaagctggga tagttgaaaa acgtacattc accgaacatc tttataacat tgacttgttc
    19381 aaaatgcaaa tggaagaaaa taaaggttgc ataccggata aagaattttc caggaataaa
    19441 atattccttg atattgaaaa catgttggat gtatctcttt catctacatt atccatgcct
    19501 tccggtaacg atacactttt acgatttgct aagtatagtg aagaggtaat ggaaaatgca
    19561 tagaagctca aacttgctgt ttctttcctt gatgttattt tctgttacag ggtgtagttt
    19621 taatcaactt cccggtacaa taattagccg gtcgcctgat aaaaagctat cacctccggg
    19681 agatacaaaa tgggttaata tcgaaataaa aaatccatca ccttatacaa aacctttccc
    19741 gttaagcgtc agatatatct cttatgaatg tcagaaaaag cgaataagtg gatttgatgg
    19801 ctctgttgtt tcggaacccg gctataatgt taaggaaatt ccgatgcagc aagagagagg
    19861 agatatctgg aaagcgaaag tagcgatgac tggtggtggt tcatgcaatt ggacattaag
    19921 tgccgttacg ctaggtattg aatataccga tgccactcat cttggtaaag gtcttgtgcc
    19981 gggtagtgca gttggtgtga ggctcgcttt tgatggttat gcttcacgaa atgggcaatt
    20041 tgattttatt ggtgggaatc agtttgatta ctatcctaaa tactatccgt tcataaagaa
    20101 atggagcgat agttcacagg gtttaatgca tga
//
LOCUS       DAZFRN010000086        19790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFRN010000086 DAZFRN010000000
VERSION     DAZFRN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.086"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..107)
                     /locus_tag="ACHKUB_002985"
     CDS             complement(<1..107)
                     /locus_tag="ACHKUB_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO6655968.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHY"
     gene            393..599
                     /gene="ybcW"
                     /locus_tag="ACHKUB_002986"
     CDS             393..599
                     /gene="ybcW"
                     /locus_tag="ACHKUB_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFO6655969.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(764..958)
                     /gene="ylcI"
                     /locus_tag="ACHKUB_002987"
     CDS             complement(764..958)
                     /gene="ylcI"
                     /locus_tag="ACHKUB_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6655970.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            1347..1892
                     /gene="nohD"
                     /locus_tag="ACHKUB_002988"
     CDS             1347..1892
                     /gene="nohD"
                     /locus_tag="ACHKUB_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO6655971.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1867..3792
                     /locus_tag="ACHKUB_002989"
     CDS             1867..3792
                     /locus_tag="ACHKUB_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6655972.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYI
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTLEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3789..3995
                     /locus_tag="ACHKUB_002990"
     CDS             3789..3995
                     /locus_tag="ACHKUB_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO6655973.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3992..5593
                     /locus_tag="ACHKUB_002991"
     CDS             3992..5593
                     /locus_tag="ACHKUB_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6655974.1"
                     /translation="MKMPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRDKLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAHFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAATFESGLRQSTEEEKSDSRAA"
     gene            5574..6905
                     /locus_tag="ACHKUB_002992"
     CDS             5574..6905
                     /locus_tag="ACHKUB_002992"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6655975.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDAHKSRLSGGRMTKETQSTTVSATASQADVTEVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNLASDTNKELLNTPEALPV"
     gene            6915..7247
                     /locus_tag="ACHKUB_002993"
     CDS             6915..7247
                     /locus_tag="ACHKUB_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6655976.1"
                     /translation="MATKEEFNHYQPLGNSDPAHTAIAPGGLSAKTPAMTPLMLDGTT
                     RKLVVWDGTTDGAAVGILAVAADQTSTTLTFYKSGSFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            7303..8328
                     /locus_tag="ACHKUB_002994"
     CDS             7303..8328
                     /locus_tag="ACHKUB_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6655977.1"
                     /translation="MSVYTTAELLASTQHHFKFDPLFLRLFFRETYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIRQNMLDENLAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8370..8756
                     /locus_tag="ACHKUB_002995"
     CDS             8370..8756
                     /locus_tag="ACHKUB_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6655978.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     ADQDTPLSPENALTGHENEVVSAQPDTVTDTAALVTVVALVTLHTDALHATRDKPVAF
                     VLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8768..9121
                     /locus_tag="ACHKUB_002996"
     CDS             8768..9121
                     /locus_tag="ACHKUB_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6655979.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9133..9711
                     /locus_tag="ACHKUB_002997"
     CDS             9133..9711
                     /locus_tag="ACHKUB_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6655980.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVAQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9708..10103
                     /locus_tag="ACHKUB_002998"
     CDS             9708..10103
                     /locus_tag="ACHKUB_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6655981.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            10111..10851
                     /locus_tag="ACHKUB_002999"
     CDS             10111..10851
                     /locus_tag="ACHKUB_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6655982.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEITVTDS"
     gene            10867..11289
                     /locus_tag="ACHKUB_003000"
     CDS             10867..11289
                     /locus_tag="ACHKUB_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6655983.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            11271..11705
                     /locus_tag="ACHKUB_003001"
     CDS             11271..11705
                     /locus_tag="ACHKUB_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6655984.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11698..14277
                     /locus_tag="ACHKUB_003002"
     CDS             11698..14277
                     /locus_tag="ACHKUB_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6655985.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            14274..14603
                     /locus_tag="ACHKUB_003003"
     CDS             14274..14603
                     /locus_tag="ACHKUB_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6655986.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14603..15301
                     /locus_tag="ACHKUB_003004"
     CDS             14603..15301
                     /locus_tag="ACHKUB_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6655987.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRRYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSEADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15307..16050
                     /locus_tag="ACHKUB_003005"
     CDS             15307..16050
                     /locus_tag="ACHKUB_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6655988.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            16047..16619
                     /locus_tag="ACHKUB_003006"
     CDS             16047..16619
                     /locus_tag="ACHKUB_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6655989.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16679..>19790
                     /locus_tag="ACHKUB_003007"
     CDS             16679..>19790
                     /locus_tag="ACHKUB_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6655990.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQGYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMVWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGNVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVS
                     KSFPRTN"
BASE COUNT         4570 a         4931 c         6122 g         4167 t
ORIGIN      
        1 atataatgtg agacgttgtg acgttttagt tcagaataaa acaattcaca gtttaaatct
       61 tttcgcactt gatcgaatat ttctttaaaa atggcaacct gagccattgg taaaaccttc
      121 catgtgatac gagggcgcgt agtttgcatt atcgttttta tcgtttcaat ctggtctgac
      181 ctctttgtgt tttgttgatg atttatgtca aatattagga atgttttcac ttaatagtat
      241 tggttgcgta acaaagtgcg gtcctgctgg cattctggag ggaaatacaa ccgacagatg
      301 tatgtaaggc caacgtgctc aaatcttcat acagaaagat ttgaagtaat attttaaccg
      361 ctagatgaag agcaagcgca tggagcgaca aaatgaataa agaacaatct gctgatgatc
      421 cctccgtgga tctgattcgt gtaaaaaata tgcttaatag caccatttct atgagttacc
      481 ctgatgttgt aattgcatgt atagaacata aggtgtctct ggaagcattc agagcaattg
      541 aggcagcgtt ggtgaagcac gataataata tgaaggatta ttccctggtg gttgactgat
      601 caccataact gctaatcatt caaactattt agcctgtgac agagccaaca cgcagtctgt
      661 cactgtcagg aaagtggtaa aactgcaact caattactgc aatgccctcg taattaagtg
      721 aatttacaat atcgtcctgt tcggagggaa gaacgcggga tgttcattct tcatcacttt
      781 taattgatgt atatgctctc ttttctgacg ttagtctccg acggcaggct tcaatgaccc
      841 aggctgagaa attcccggac cctttttgct caagagcgat gttaatttgt tcaatcattt
      901 ggttaggaaa gcggatgttg cgggttgttg ttctgcgggt tctgttcttc gttgacatga
      961 ggttgccccg tattcagtgt cgctgatttg tattgtctga agttgttttt acgttaagtt
     1021 gatgcagatc aattaatacg atacctgcgt cataattgat tatttgacgt tgtttgatgg
     1081 cgtagatgca cgttgtgaca tgtagatgat aattattatc attttgcggg tcctttccgg
     1141 cgatccgaca ggttacgggg cggcgacctc gcgggttttc gctatttatg aaaattttcc
     1201 ggtttaaggc gtttccgttc ttcttcgtcg taacttaatg tttttattta aaataccccc
     1261 tgaaaagaaa ggaaacgaca ggtgctgaaa acgaactttt gggcctttgt cgtttccttt
     1321 ctctgttttt ggccgtggaa tgaacaatgg aagtcaacaa aaagcagctg gctgacattt
     1381 tcggtgcgag tatccgtacc attcagaact ggcaggaaca gggaatgccc gttctgcgag
     1441 gcggtggcaa gggtaatgag gtgctttatg actctgccgc cgttataaaa tggtatgccg
     1501 aaagggatgc tgaaattgag aacgaaaagc tgcgccggga ggttgaagaa ctgcgccagg
     1561 ccagcgaggc agatctccag ccagggacta ttgagtacga acgccatcga cttacgcgtg
     1621 cgcaggccga cgcacaggaa ctgaagaatg ccagagactc agctgaagtg gtggaaaccg
     1681 cattctgtac tttcgtgctg tcgcggatcg caggtgaaat tgccagtatt ctcgacggga
     1741 tccccctgtc ggtgcagcgg cgttttccgg aactggaaaa ccgacatgtt gatttcctga
     1801 aacgggatat catcaaagcc atgaacaaag cagccgcgct ggatgaactg ataccggggt
     1861 tgctgagtga atatatcgaa cagtcaggtt aacaggctgc ggcattttgt ccgcgccggg
     1921 cttcgctcac tgttcaggcc ggagccacag accgccgttg aatgggcgga tgccaattac
     1981 tatctcccga aagaatccgc ataccaggaa gggcgctggg aaacactgcc ctttcagcgg
     2041 gccatcatga atgcgatggg cagcgactac atccgtgagg tgaatgtggt gaagtctgcc
     2101 cgtgtcggtt attccaaaat gctgttgggt gtttatgcct acttcataga gcataagcag
     2161 cgcaacaccc ttatctggtt gccgacggat ggtgatgccg agaactttat gaaaacccac
     2221 gttgagccga ctattcgtga tattccgtcg ctgctggcgc tggccccgtg gtatggcaaa
     2281 aagcaccggg ataacacgct caccatgaag cgtttcacta atgggcgtgg cttctggtgc
     2341 ctgggcggta aagcggcaaa aaactaccgt gaaaagtcgg tggatgtggc gggttatgat
     2401 gaacttgctg cttttgatga tgatattgaa caggaaggct ctccgacgtt cctgggtgac
     2461 aagcgtattg aaggctcggt ctggccaaag tccatccgtg gctccacgcc caaagtgaga
     2521 ggcacctgtc agattgagcg tgcagccagt gaatccccgc attttatgcg ttttcatgtt
     2581 gcctgcccgc actgcgggga ggagcagtac cttaaatttg gcgataaaga gacgccgttt
     2641 ggcctcaaat ggacgccgga tgatccctcc agcgtgtttt atctctgcga acataatgcc
     2701 tgcgtcatcc gccagcagga gctggacttc actgatgccc gttatatctg cgaaaagacc
     2761 gggatctgga cccgtgatgg cattctctgg ttttcgtcat ccggtgaaga gattgagccg
     2821 ccggacagcg tgacctttca catctggacg gcgtacagcc cgttcaccac ctgggtgcag
     2881 attgtcaaag actggatgaa aacgaaaggg gatacgggaa aacgtaaaac cttcgtgaac
     2941 accacgctcg gtgagacatg ggaagcgaaa atcggtgaac gtccggatgc tgaagtgatg
     3001 gcagagcgga aagagcatta ttcagcgccc gttcctgacc gtgtggctta cctgactgcc
     3061 ggtatcgact cccagctgga tcgctacgaa atgcgcgtat ggggatgggg gccgggtgag
     3121 gaaagctggc tgattgaccg gcagattatt atgggccgcc acgatgatga acagacgctg
     3181 ctgcgtgtgg atgaggccat caataaaacc tatatccgcc ggaatggtgc agaaatgtcg
     3241 gtatcccgta tctgctggga tactggcggg attgacccga ccattgtgta tgaacgctcg
     3301 aaaaagcatg ggctgttccg ggtgatcccc attaaagggg catccgtcta cggaaagcct
     3361 gtggccagca tgccacgtaa gcgaaacaaa aacggggttt accttaccga aattggtacg
     3421 gataccgcga aagagcagat ttataaccgc ttcacactga cgctggaagg ggatgaaccg
     3481 cttcccggtg ccgttcactt cccgaataac ccggatattt ttgatctgac cgaagcgcag
     3541 cagctgactg ctgaagagca ggtcgaaaaa tgggtggatg gcaggaaaaa aatactgtgg
     3601 gacagcaaaa agcgacgcaa tgaggcgctc gactgcttcg tttatgcgct ggcggcgctg
     3661 cgcatcagta tttcccgctg gcagctggat ctcagtgcgc tgctggcgag cctgcaggaa
     3721 gaggatggtg cagcaaccaa caagaaaaca ctggcagatt acgcccgtgc cttatccgga
     3781 gaggatgaat gacgcgacag gaagaacttg ccgctgcccg tgcggcactg catgacctga
     3841 tgacaggtaa acgggtggca acggtacaga aagacggacg gcgagtggag tttacggcca
     3901 cttccgtgtc tgacctgaaa aaatatattg cagagctgga agtgcagacc ggcatgacac
     3961 agcgacgcag gggacctgca ggattttatg tatgaaaatg cccaccattc ccacccttct
     4021 ggggccggac ggcatgacat cgctgcgtga atatgccggt tatcatggcg gtggcagcgg
     4081 atttggtggg cagttgcggg cgtggaatcc accgagtgaa agtgtggatg cagccctgct
     4141 gcccaacttt acccgtggca atgcccgcgc agacgatctg gtgcgcaata acggctatgc
     4201 cgccaacgcc atccagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     4261 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagac gcccgtgcct tttcccgcga
     4321 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     4381 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     4441 actgttcgtt caggccacct gggataccag ttcgtcgcgg ctgttccgga cacagttccg
     4501 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     4561 tgccggtgtg cagattaatg acagcggcgc ggcgctggga tattacgtca gcgaggacgg
     4621 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     4681 cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt
     4741 gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca cgcagctgca
     4801 gagcgccatt gtgaaggcga tgtatgccgc caccattgaa agtgagctgg atacgcagtc
     4861 agcgatggat tttattctgg gcgcgaacag tcaggagcag cgggacaagc tgacgggctg
     4921 gattggtgaa attgccgcgt attacgccgc cgcgccggtc cggctgggag gcgcaaaagt
     4981 gccacacctg atgccgggtg actcactgaa cctgcagacg gctcaggaca cggataacgg
     5041 ctactccgtg tttgagcagt cactgctgcg gtatatcgct gccgggctgg gtgtctcgta
     5101 tgagcagctt tccaggaatt acgcccagat gagctactcc acggcacggg ccagcgcgaa
     5161 cgagtcgtgg gcgcacttta tgggacggcg aaaattcgtc gcatcccgtc aggcgagcca
     5221 gatgtttctg tgctggctgg aagaggccat cgttcgccgc gtggtgacgt taccttcaaa
     5281 agcgcgcttc agttttcagg aagcccgcag tgcctggggg aactgcgact ggataggctc
     5341 cggtcgtatg gccatcgatg gtctgaaaga agtacaggaa gcggtgatgc tgatagaagc
     5401 cggactgagt acctacgaga aagagtgcgc aaaacgcggc gacgattatc aggaaatttt
     5461 tgcccagcag gtccgtgaaa cgatggagcg ccgcgcagcc ggtcttaaac cgcccgcctg
     5521 ggcggcagcg acatttgaat ccggactgcg acaatcaaca gaggaggaga agagtgacag
     5581 cagagctgcg taatctcccg catattgcca gtatggcctt taatgagccg ctgatgcttg
     5641 aacccgccta tgcgcgggtt ttcttttgtg cgcttgcagg ccagcttggg atcagccgcc
     5701 tgacggatgc ggtgtccggc gacagcctga ctgcccagga ggcactcgcg acgctggcat
     5761 tatccggtga tgatgacgga ccacgacagg cccgcagtta tcaggtcatg aacggcatcg
     5821 ccgtgctgcc ggtgtccggc acgctggtca gccggacgcg ggcgctgcag ccgtactcgg
     5881 ggatgaccgg ttacaacggc attatcgccc gtctgcaaca ggctgccagc gacccgatgg
     5941 tggacggcat tctgctcgat atggacacgc caggcggaat ggtggcgggg gcatttgact
     6001 gcgctgacat catcgcccgt gtgcgtgaca taaaaccggt atgggcgctg gccaacgaca
     6061 tgaactgcag tgcaggtcag ctgcttgcca gtgccgcctc ccggcgtctg gtcacgcaga
     6121 ccgcccggac aggctccatc ggcgtcatga tggctcacag taattacggt gctgccctgg
     6181 agaaacaggg cgtggaaatc acgctgattt acagcggcag ccataaggtg gatggcaatc
     6241 cttacagcca tcttccggat gacgtccggg agacactgca gtcccggatg gacgcaaccc
     6301 gccagatgtt tgcgcagaag gtgtcggcat ataccggcct gtctgtgcag gctgtgctgg
     6361 ataccgaggc tgcagtgtac agcggtcagg aggccattga tgccggactg gctgatgaac
     6421 ttgttaacag caccgatgcg atcaccgtca tgcgtgatgc actggatgca cataaatccc
     6481 gtctctcagg agggcgaatg accaaagaga ctcaatcaac aactgtttca gccactgctt
     6541 cgcaggctga cgttactgag gtggtgccag cgacggaggg cgaaaacgcc agtgcggcgc
     6601 agccggacgt gaacgcgcag atcactgcag cggttgcggc agaaaacagc cgcattatgg
     6661 ggatcctcaa ctgtgaggag gctcacggac gcgaagaaca ggcgcgcgtt ctggcagaaa
     6721 cccccggtat gaccgtggaa acggcccgcc gcattctggc cgcagcacca cagagtgcac
     6781 aggcgcgcag tgacactgcg ctggatcgtc tgatgcaggg tgcaccggca ccgctggctg
     6841 caggtaacct ggcatctgat accaataaag aattacttaa tacacctgaa gctttaccgg
     6901 tataagaggc agttatggcg acaaaagaag agtttaacca ttaccagccg ctgggtaaca
     6961 gtgatccggc tcatacagca attgcgcctg gcggattgag tgcgaaaacg cctgcaatga
     7021 ccccactgat gctggatggc actacccgta agctggttgt gtgggatggc accaccgacg
     7081 gtgcagccgt tggcattctg gcggttgctg ctgaccagac cagcaccaca ctgacgttct
     7141 acaagtccgg ctcgttccgt tatgaggatg tgctctggcc ggaggctgcc agcgacgaga
     7201 cgaaaaaacg gaccgcgttt gccggaacgg caatcagcat cgtttaatct tccccttcat
     7261 caacaaaggc cgcctgtgcg gcttttttta tggaaataat ttatgtctgt atatacaact
     7321 gcagaattac tggcatcgac ccagcatcac tttaagttcg atccgctgtt tctgcgcctg
     7381 tttttccgtg aaacctatcc tttcaccacg gaaaaagtct atctctcaca aattccggga
     7441 ctggtaaaca tggcgctgta cgtttcgccg attgtttccg gtgaggttat ccgatcccgt
     7501 ggcggctcca cctctgaatt tacgccgggt tatgtcaagc cgaagcatga ggtgaatccg
     7561 cagatgaccc tgcgtcgcct gccggatgaa gatccgcaga acctggctga cccggcttac
     7621 cgtcgtcgcc gtattattcg gcagaatatg ctggatgaaa atctggcgat tgcccaggtc
     7681 gaagagatgc aggcagtttc tgccgtgctt aagggcaaat ataccatgac cggtgaagcc
     7741 ttcgatccgg ttgaggtgga tatgggccgc agtgaggcga ataacatcac gcagtccggc
     7801 ggcacggagt ggagcaagcg tgacaagtcc acgtatgacc cgaccgacga tatcgaagcc
     7861 tacgcgctga acgccagcgg tgtggtgaat atcatcgtgt ttgatccgaa aggctgggcg
     7921 ctgttccgtt ccttcaaagc cgtcaaggag aagctggata cccgtcgcgg ctctcattcc
     7981 gagctggaga cagcggtaaa agacctgggc aaagcggtgt cctataaggg aatgtatggc
     8041 gatgtggcca tcgtcgtgta ttccggacag tacgtggaaa acggcgtcaa aaagaacttc
     8101 ctgccggaca acacgatggt gctggggaac actcaggctc gcggtctgcg cacctatggc
     8161 tgcattcagg atgcggacgc acagcgcgaa ggcattaacg cctctgcccg ttacccgaaa
     8221 aactgggtga ctaccggcga tccggcgcgt gagttcacca tgattcagtc agcaccgctg
     8281 atgctgctgg ctgatcctga tgagttcgtg tccgttcaac tggcgtaatc atggcccttc
     8341 ggggccattt tctctctgtg gaggagttca tgacgaaaga tgaactgatt gcccgtcttc
     8401 aggagctggg tgagcaactg aaccgcgatg tcagtttgac gggaacgaaa gaagaactgg
     8461 cgctccgtgt ggcagagctg gaagaggagc ttgatgacac tgccgatcag gatacccctc
     8521 tcagcccgga aaatgcgctg accggacatg aaaatgaggt ggtatcagcg cagccggata
     8581 ccgtgactga tacggctgct ctggtcacgg ttgtggcact ggtgacgctg cataccgatg
     8641 cacttcacgc cacgcgggat aaacctgtgg catttgtgct gccgggaacg gcgtttcgtg
     8701 tctctgccgg tgtggcagcc gaaatgacag aacgtggcct ggccagaatg caataacggg
     8761 aggcgctgtg gctgatttcg ataacctgtt cgatgctgcc attgcccgcg ctgatgaaac
     8821 gatacgcggg tacatgggaa cgtcagccac catgacatcc ggtgagcagt ccggtgctgt
     8881 gatacgtggt gtttttgatg accctgaaaa tatcagctat gccggacagg gcgtgcgcgt
     8941 tgaaggctcc agcccgtccc tgtttgtccg gactgatgat gtgcggcagc tgcggcgcgg
     9001 cgacacgctg accatcggtg aggaaaactt ctggatagac cggatttcgc cggatgatgg
     9061 cggaagctgt catctctggc ttgggcgggg cgtaccgcct gccgttaacc gtcgccgctg
     9121 aaagggggat gtatggccat aaaaggtctt gagcaggccg ttgaaaacct cagccgtatc
     9181 agcagaacgg cggtgcccgg tgccgccgca atggccatta accgcgttgc ttcatccgcg
     9241 atatcgcagt cggtggcaca ggttgcccgt gagacaaagg tacgccggaa actggtaaag
     9301 gaaagggcca ggctgaaaag ggccacggtc aaaaatccgc aggccagaat cagggttaac
     9361 cggggggatt tgcccgtaat caagctgggt aacgcgcggg ttgtcctttc gcgccgcagg
     9421 cgtcgtaaaa aggggcagcg ttcatccctg aaaggtggcg gcagcgtgct tgtggtggga
     9481 aaccgtcgta ttcccggcgc gtttattcag caactgaaaa atggccggtg gcatgtcatg
     9541 cagcgtgtgg ccgggaaaaa ccgttacccc attgatgtgg tgaaaatccc gatggcggtg
     9601 ccgcttacca cggcgtttaa acagaatatt gaacggatac ggcgtgaacg tcttccgaaa
     9661 gagctgggct atgcgctgca gcatcaactg agaatggtaa taaagcgatg aaacatactg
     9721 aactccgtgc agccgtactg gatgcactgg agaagcatga caccggggcg acgctttttg
     9781 atggtcgccc cgctgttttt gatgaggcgg attttccggc aattgccgtt tatctcaccg
     9841 gcgctgaata cacgggcgaa gagctggaca gtgatacctg gcaggcggag ctgcatattg
     9901 aagttttcct gcctgctcag gtgccggatt cagagctgga ttcgtggatg gagtcccgga
     9961 tttatccggt gatgagcgat gtcccggcac tgtcagattt gatcaccagt atggtggcca
    10021 gtggctatga ctaccggcgc gacgatgatg cgggcctgtg gagttcagcc gatctgactt
    10081 atgtcattac ctatgaaatg tgaggacgat atgcctgtac caaatccagt aatgccggtg
    10141 aaaggggccg ggaccacact gtgggtttat aaggggaacg gtgaccctta tgcgaacccg
    10201 ctttcagacg ttgactggtc gcgtctggct aaagttaaag acctgacgcc cggcgaactg
    10261 accgctgagt cctatgacga cagctatctc gatgatgaag atgcggactg gactgcgacc
    10321 gggcaggggc agaaatctgc cggagatacc agcttcacgc tggcgtggat gcccggagag
    10381 caggggcagc aggcgctgct ggcgtggttt aatgaagggg atacccgtgc ctataaaatc
    10441 cgcttcccga acggcacggt cgatgtgttc cgcggctggg tcagcagtat cggtaaggcg
    10501 gtgacggcga aggaagtgat cacccgcacg gtgaaagtca ccaacgtggg acgtccgtcg
    10561 atggcagaag atcgcagcac ggtaacagcg gcaaccggca tgaccgtgac gcctgccagc
    10621 acttcggtgg tgaaagggca gagcaccacg ctgaccgtgg cattccagcc ggaaggcgca
    10681 accgacaaga gcttccgtgc ggtgtctgcg gataaaacaa aagccaccgt gtcggtcagt
    10741 ggtatgacca tcaccgtgaa aggtgttgct gcaggcaagg tcaacattcc ggttgtatcc
    10801 ggtaatggtg agtttgctgc ggttgcagaa atcaccgtca ccgacagtta atccggagag
    10861 tcagcgatgt tcctgaaaac cgaatcattt gaatataacg gtgtgagcgt cacgctttct
    10921 gaactgtcag ccctgcagcg aattgagcat ctcgccctgc tgaaacgaca ggcagaacag
    10981 gcgggatcca gtctcaatcg acaggtgagc gtggaagatc tcgtcagaac cggtgctttt
    11041 ctggtggcga tgtccctgtg gcatagccat ccgcagaaga caaagatgcc gtccatgaat
    11101 gaagccgtta aacaaattga gcaggaagtg cttaccacct ggcccacaga ggcaattgct
    11161 caggctgaaa atgtggtaat gcgtctgtcc ggtatgtctg agtttgttgt gaatgatgca
    11221 cctgaacagg cagatgacgc cgggccagca gagcctgttt ctgcgggaaa gtgttcgacg
    11281 gtgagctgag ttttgccctg aaactggcgc gtgagatggg gcgacccgac tggcgcgcca
    11341 tgcttgccgg gatgtcatcc acggagtatg ccgactggca ccgcttttac agtacccatt
    11401 attttcatga tgttctgctg gatatgcact tttccgggct gacgtacacc gtactcagcc
    11461 tgtttttcag cgatccggat atgcatccgc tggatttcag tctgctgaac cggcgcgagg
    11521 ctgacgaaga gcctgaagat gatgtgctga tgcagaaagc ggcagggctt gccggaggcg
    11581 tccgctttgg cccggacggg aatgaagtta tccccgcttc cccggatgtg gcggacatga
    11641 cggaggatga cgtaatgctg atgacagtat cagaagggat cgcaggagga gtccggtatg
    11701 gctgaaccgg taggcgatct ggtcgttgat ttgagtctgg atgcggccag atttgacgag
    11761 cagatggcca gagtcaggcg tcatttttcc ggtacggaaa gtgatgcgaa aaaaacagcg
    11821 gcagtcgttg aacagtcaat gaaccggcag gcgctggctg cacagaaagc ggggatttcc
    11881 gtcggacagt ataaagccgc catgcgtatg ctgcctgcac agttcaccga cgtggccacg
    11941 cagcttgcag gcgggcaaag cccgtggctg atcctgctgc aacagggggg tcaggttaag
    12001 gactccttcg gcgggatgat ccccatgttc agggggcttg ccggtgcgat caccctgccg
    12061 atggtggggg ccacctcgct ggcggtggcg accggtgcgc tggcgtatgc ctggtatcag
    12121 ggcaactcaa ccctgtccga tttcaacaaa acgctggtcc tttccggcaa tcaggcggga
    12181 ctgacggcag atcgtatgct ggtcctgtcc agagccgggc aggcggcagg gctgacgttt
    12241 aaccagacca gcgagtcact gacggcgctg gtgaatgccg gtgtgcgtgg tggtgagcag
    12301 tttgaggcga tcagccagag tgtggcgcgt ttctcctctg catccggcgt ggaggtggac
    12361 aaggtcgctg aagccttcgg gaagctgacc acagacccga cgtcgggact gacagcgatg
    12421 gcacgtcagt tccataacgt gacggcggag cagattgcgt atgttgctca gttgcagcgt
    12481 tccggagatg aagccggggc attgcaggcg gcgaacgagg ccgcaacgaa agggtttgat
    12541 gaccagaccc gccgcctgaa agagaacatg ggcacgctgg agacctgggc agacaggaca
    12601 gcacgggcat tcaaatccat gtgggattcg gtgctggata ttggtcgccc ggacactgcc
    12661 cagggaatgc tggagaaagc agaaaaggct tttgatgagg cggacaaaaa atggcagtgg
    12721 tatcagagcc ggagccaccg gcgcggtaaa acctcagcat ttcttgccaa tctccgggga
    12781 gcatgggagg acagagcgaa tgcgcaactt ggactttcag ccgccacgtt gcaggccgat
    12841 cttgaaaagg ccagagagat ggcagcaaag gactgggccg agtctgaggc atcacggctg
    12901 aaatataccg aagaggcgca gaaggcttac gaacggctgc agacgccgct ggagaaatat
    12961 accgcccgtc aggaagaact gaacaaggca ctgaaagacg ggaaaatcct gcaggcggat
    13021 tacaacacgc tgatggcggc ggcgaaaaag gattatgaag cgacgctgaa aaagccgaaa
    13081 cagtccggcg tgaaggtgtc tgcgggcgat cgtcaggaag acagtgctca tgctgccctg
    13141 ctgacgcttc aggcagaact ccggacgctg gagaagcatg ccggagcaaa tgagaaaatc
    13201 agccagcagc gccgggattt gtggaaggcg gagagtcagt tcgcggtact ggaggaggcg
    13261 gcgcaacgtc gccagctgtc tgcacaggag aaatccctgc tggcgcataa agatgagacg
    13321 ctggagtaca aacgccagct ggctgcactt ggcgacaagg ttacgtatca ggagcgcctg
    13381 aacgcgctgg cgcagcaggc ggataaattc gcacagcagc aacgggcaaa acgggccgcc
    13441 attgatgcga aaagccgggg gctgactgac cggcaggcag aacgggaagc cacggaacag
    13501 cgcctgaagg aacagtatgg cgataatccg ctggcgctga ataacgtcat gtcagagcag
    13561 aaaaagacct gggcggctga agaccagctt cgcgggaact ggatggcagg cctgaagtcc
    13621 ggctggagtg agtgggaaga gagcgccacg gacagtatgt cgcaggtaaa aagtgcagcc
    13681 acgcagacct ttgatggtat tgcacagaat atggcggcga tgctgaccgg cagtgagcag
    13741 aactggcgca gcttcacccg ttccgtgctg tccatgatga cagaaattct gcttaagcag
    13801 gcaatggtgg ggattgtcgg gagtatcggc agcgccattg gcggggctgt tggtggcggc
    13861 gcatccgcgt caggcggtac agccattcag gccgctgcgg cgaaattcca ttttgcaacc
    13921 ggaggattta cgggaaccgg cggcaaatat gagccagcgg ggattgttca ccgtggtgag
    13981 tttgtcttca cgaaggaggc aaccagccgg attggcgtgg ggaatcttta ccggctgatg
    14041 cgcggctatg ccaccggcgg ttatgtcggt acaccgggca gcatggcaga cagccggtcg
    14101 caggcgtccg ggacgtttga gcagaataac catgtggtga ttaacaacga cggcacgaac
    14161 gggcagatag gtccggctgc tctgaaggcg gtgtatgaca tggcccgcaa gggtgcccgt
    14221 gatgaaattc agacacagat gcgtgatggt ggcctgttct ccggaggtgg acgatgaaga
    14281 ccttccgctg gaaagtgaaa cccggtatgg atgtggcttc ggtcccttct gtaagaaagg
    14341 tgcgctttgg tgatggctat tctcagcgag cgcctgccgg gctgaatgcc aacctgaaaa
    14401 cgtacagcgt gacgctttct gtcccccgtg aggaggccac ggtactggag tcgtttctgg
    14461 aagagcacgg gggctggaaa tcctttctgt ggacgccgcc ttatgagtgg cggcagataa
    14521 aggtgacctg cgcaaaatgg tcgtcgcggg tcagtatgct gcgtgttgag ttcagcgcag
    14581 agtttgaaca ggtggtgaac tgatgcagga tatccggcag gaaacactga atgaatgcac
    14641 ccgtgcggag cagtcggcca gcgtggtgct ctgggaaatc gacctgacag aggtcggtgg
    14701 agaacgttat tttttctgta atgagcagaa cgaaaaaggt gagccggtca cctggcaggg
    14761 gcgacggtat cagccgtatc ccattcaggg gagtggtttt gaactgaatg gcaaaggcac
    14821 cagtacgcgc cccacgctga cggtttctaa cctgtacggt atggtcaccg ggatggcgga
    14881 agatctgcag agtctggtcg gcggaacggt ggtccggcgt aaggtttacg cccgttttct
    14941 ggatgcggtg aacttcgtca acggaaacag cgaagccgat ccggagcagg aggtgatcag
    15001 ccgctggcgc attgagcagt gcagcgaact gagcgcggtg agtgcctctt ttgtactgtc
    15061 cacgccgacg gaaacggacg gcgctgtttt tccgggacgt atcatgctgg ccaacacctg
    15121 cacctggacc tatcgcggtg acgagtgcgg ttatagcggt ccggctgtcg cggatgaata
    15181 tgaccagcca acgtccgata tcacgaagga taaatgcagc aaatgcctga gcggttgtaa
    15241 gttccgcaat aacgttggca actttggcgg cttcctttcc attaacaaac tttcgcagta
    15301 aatcccatga cacagacaga atcagcgatt ctggcgcacg cccggcgatg tgcgccagcg
    15361 gagtcgtgcg gcttcgtggt aagcacgccg gagggggaaa gatatttccc ctgcgtgaat
    15421 atctccggtg agccggaggc gtatttccgt atgtcgccgg aagactggct gcaggcagaa
    15481 atgcagggtg agattgtggc gctggtccac agccaccccg gtggtctgcc ctggctgagt
    15541 gaggccgacc ggcggctgca ggtgcagagt gatttgccgt ggtggctggt ctgccggggg
    15601 acgattcata agttccgctg tgtgccgcat ctcaccgggc ggcgctttga gcacggtgtg
    15661 acggactgtt acacactgtt ccgggatgct tatcatctgg cggggattga gatgccggac
    15721 ttttatcgtg aggatgactg gtggcgtaac ggccagaatc tctatctgga taatctggag
    15781 gccacagggc tgtatcaggt gccgttgtca tcagcacaac cgggcgatgt gctgctgtgc
    15841 tgttttggtt catcggtgcc gaatcatgcc gccatttact gtggtgatgg cgagctgctg
    15901 caccatattc ctgaacaact gagcaaacga gagaggtata ccgacaaatg gcagcgacgc
    15961 acacactccc tctggcgtca ccgggcatgg cgcgcatctg cctttacggg gatttacaac
    16021 gatttggccg ccgcatcgac cttcgtgtga aaacgggggc tgaagccatc cgggcactgg
    16081 ccacacagct cccggcgttt cgtcagaaac tgagcgacgg ctggtatcag gtacggattg
    16141 ccgggcagga tgtcagcacg tccggattaa cggcgcagtt acatgagact ctgcctgatg
    16201 gcgctgtgat tcatattgtt cccagagtcg ccggggccaa gtcaggtggc gtattccaga
    16261 ttgtcctggg ggctgccgcc attgccggat cattctttac cgccggggcc acccttgcag
    16321 catggggggc agccattggg gccggtggta tgaccggcat cctgttttct ctcggtgcca
    16381 gtatggtgct cggtggtgtg gcgcagatgc tggcaccgaa agccagaact ccccgtacac
    16441 agacaacgga taacggcaaa cagaacacct atttctcctc actggataac atggttgccc
    16501 agggcaatgt tctgccggtt ctgtacggtg aaatgcgcgt ggggtcgcgg gtggtctctc
    16561 aggagatcag cacggcagac gaaggggatg gtggtcaggt tgtggtgatt ggtcgctgat
    16621 gaaaaacgtt tatgtgaaac cgcctccggg cggttttgtc gtttatggag cgtgaggaat
    16681 gggtaaaggc agcagtaagg ggcatactcc gcgcgaagcg aaggacaacc tgaagtccac
    16741 gcagttgctg agtgtgatcg atgccatcag cgaagggccg gttgaaggtc cggtggatgg
    16801 attaaaaagc gtgctgctga acagtacgcc agtgctggac agtgagggga ataccaatat
    16861 ctccggcgtc acggtggtgt tccgggccgg tgagcaggag cagacaccgc cggagggatt
    16921 tgaatcctcc ggctccgaga cggtgctggg tacggaagtg aaatacgaca cgccgatcac
    16981 ccggaccatc acgtcggcaa acattgaccg actgcgtttt accttcggcg tgcaggcact
    17041 ggtggaaacc acctcaaagg gggacaggaa tccgtcggaa gtccgcctgc tggttcagat
    17101 ccagcgtaat ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacctc
    17161 gcagtatctg gcctcggtgg tggtgggtaa cctgccgccg cgcccgttca atatccggat
    17221 gcgcaggatg acgccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc
    17281 atacaccgaa atcatcgatg tgaaacaggg ctacccgaac acggcactgg tcggcgtgca
    17341 ggtggattcg gagcagttcg gcagccagca ggtgagccgt aattatcatc tgcgcgggcg
    17401 cattctgcag gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga
    17461 cggaacgttt aaaccggcat acagcaacaa catggtctgg tgtctgtggg atatgctgac
    17521 ccatccgcgc tacggcatgg ggaaacgtct tggtgcggcg gatgtggata aatgggcgct
    17581 gtatgtcatc ggccagtact gcgaccagtc agtgccggac ggctttggcg gcacggagcc
    17641 gcgcatcacc tgtaatgcgt acctgaccac acagcgcaag gcgtgggatg tgctcagtga
    17701 tttctgctcg gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca
    17761 ggaccgacca tcagataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga
    17821 tggcgcgccg ttccgctaca gcttcagcgc cctgaaggac cgccataatg ccgttgaggt
    17881 gaactggatt gacccggata acggctggga gacggcgaca gagcttgtgg aggacacgca
    17941 ggccattgcc cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg
    18001 ggggcaggca catcgcgccg ggctgtggct gattaaaaca gaactgctgg aaacgcagac
    18061 cgtggacttc agcgtgggcg cagaagggct tcgccatgtg ccgggcgatg tcattgaaat
    18121 ctgtgatgat gactatgccg gtatcagcat cggcgggcgc gtgctggcgg taaacagcca
    18181 gacccggacg ctgacgctcg accgtgaaat cacgctgcca tcctccggta ccacgctgat
    18241 aagcctggtt gacggaagtg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg
    18301 cgtgaaggtg aaagtgagcc gtgttcctga cggcgttgct gaatacagcg tgtgggggct
    18361 gaagctgccg acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga
    18421 cggcacgtat gccatcaccg ccgtgcagca tgtaccggaa aaagaagcca tcgtggataa
    18481 cggggcgcac tttgacggcg accagagcgg cacggtgaat ggtgtcacgc cgccagcggt
    18541 gcagcacctg actgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgctg
    18601 ggacacgccg aaggtggtga agggcgtgag cttcctgctc cgtctgaccg taacagcgga
    18661 tgacggcagt gagcggctgg tcagcacggc ccggacgacg gaaaccactt accgcttcac
    18721 acaactggcg ctggggaact acaggctgac agtccgggca gtaaatgcgt gggggcagca
    18781 gggcgatccg gcgtcggtat cgttccggat tgccgcaccg gcagcgccgt cgcggattga
    18841 gctgacgccg ggctattttc agataaccgc cacgccgcat cttgcggttt atgatccgac
    18901 ggtacagttt gagttctggt tctcggaaaa gcggattgcg gatatcaggc aggttgaaac
    18961 cacagcacgc tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaaacc
    19021 gggccatgat tattactttt atatccgcag tgtgaacacc gttggcaaat cggcattcgt
    19081 ggaggctgtt ggtcagccga gtgatgatgc atccggttat ctggattttt tcaaaggcga
    19141 gatagggaaa acccatctgg ctcaggagct gtggacgcag attgataacg gtcagcttgc
    19201 gcctgacctg gctgaaatca ggacgtccat tacggatgtc agcaatgaaa tcacgcagac
    19261 cgtcaataag aaactggaag accagagtgc tgcaattcag cagatacaga aggttcaggt
    19321 tgatacaaat aataacctga acagcatgtg ggctgtgaag ctgcagcaga tgcaggacgg
    19381 acgcctttat atcgcgggta ttggtgccgg tattgagaac acccctgacg gcatgcagag
    19441 tcaggtgctg ctggcggcgg acaggattgc gatggttaat cctgcgaatg gcaacacaaa
    19501 accgatgttt gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct
    19561 gacggctccc accattacca gcggtggaaa tccgccggca ttttccctga caccagacgg
    19621 aaagctgacc gctaaaaatg cggatatcag cggtaatgtg aatgcaaatt cagggacgct
    19681 caacaatgtc acgattaatg aaaactgtca gattaagggg aaactgtcag ccaatcagat
    19741 tgaaggcgat attgtcaaaa cggtcagcaa gtctttcccc cgcacgaaca
//
LOCUS       DAZFRN010000087        19549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFRN010000087 DAZFRN010000000
VERSION     DAZFRN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.087"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            209..1303
                     /gene="gcvT"
                     /locus_tag="ACHKUB_003008"
     CDS             209..1303
                     /gene="gcvT"
                     /locus_tag="ACHKUB_003008"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO6655991.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1327..1716
                     /gene="gcvH"
                     /locus_tag="ACHKUB_003009"
     CDS             1327..1716
                     /gene="gcvH"
                     /locus_tag="ACHKUB_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO6655992.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1835..4708
                     /gene="gcvP"
                     /locus_tag="ACHKUB_003010"
     CDS             1835..4708
                     /gene="gcvP"
                     /locus_tag="ACHKUB_003010"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO6655993.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            4974..5717
                     /gene="ygfF"
                     /locus_tag="ACHKUB_003011"
     CDS             4974..5717
                     /gene="ygfF"
                     /locus_tag="ACHKUB_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6655994.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTSE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5774..7207)
                     /gene="bglA"
                     /locus_tag="ACHKUB_003012"
     CDS             complement(5774..7207)
                     /gene="bglA"
                     /locus_tag="ACHKUB_003012"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO6655995.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7252..7563
                     /gene="yqfB"
                     /locus_tag="ACHKUB_003013"
     CDS             7252..7563
                     /gene="yqfB"
                     /locus_tag="ACHKUB_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO6655996.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7727..8386
                     /gene="yqfA"
                     /locus_tag="ACHKUB_003014"
     CDS             7727..8386
                     /gene="yqfA"
                     /locus_tag="ACHKUB_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO6655997.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8463..9443)
                     /gene="ygfZ"
                     /locus_tag="ACHKUB_003015"
     CDS             complement(8463..9443)
                     /gene="ygfZ"
                     /locus_tag="ACHKUB_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO6655998.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9686..9952
                     /gene="sdhE"
                     /locus_tag="ACHKUB_003016"
     CDS             9686..9952
                     /gene="sdhE"
                     /locus_tag="ACHKUB_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO6655999.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9933..10340
                     /gene="ygfX"
                     /locus_tag="ACHKUB_003017"
     CDS             9933..10340
                     /gene="ygfX"
                     /locus_tag="ACHKUB_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO6656000.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10380..10901)
                     /gene="fldB"
                     /locus_tag="ACHKUB_003018"
     CDS             complement(10380..10901)
                     /gene="fldB"
                     /locus_tag="ACHKUB_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO6656001.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11013..11909
                     /gene="xerD"
                     /locus_tag="ACHKUB_003019"
     CDS             11013..11909
                     /gene="xerD"
                     /locus_tag="ACHKUB_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO6656002.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11934..12644
                     /gene="dsbC"
                     /locus_tag="ACHKUB_003020"
     CDS             11934..12644
                     /gene="dsbC"
                     /locus_tag="ACHKUB_003020"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO6656003.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12650..14383
                     /gene="recJ"
                     /locus_tag="ACHKUB_003021"
     CDS             12650..14383
                     /gene="recJ"
                     /locus_tag="ACHKUB_003021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO6656004.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14474..15572
                     /gene="prfB"
                     /locus_tag="ACHKUB_003022"
     CDS             join(14474..14548,14550..15572)
                     /gene="prfB"
                     /locus_tag="ACHKUB_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO6656005.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15582..17099
                     /gene="lysS"
                     /locus_tag="ACHKUB_003023"
     CDS             15582..17099
                     /gene="lysS"
                     /locus_tag="ACHKUB_003023"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6656006.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17142..17690)
                     /gene="idi"
                     /locus_tag="ACHKUB_003024"
     CDS             complement(17142..17690)
                     /gene="idi"
                     /locus_tag="ACHKUB_003024"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO6656007.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17745..17816
                     /locus_tag="ACHKUB_003025"
     CDS             17745..17816
                     /locus_tag="ACHKUB_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656008.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17813..17938)
                     /gene="yqfG"
                     /locus_tag="ACHKUB_003026"
     CDS             complement(17813..17938)
                     /gene="yqfG"
                     /locus_tag="ACHKUB_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO6656009.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17940..19388)
                     /gene="uacT"
                     /locus_tag="ACHKUB_003027"
     CDS             complement(17940..19388)
                     /gene="uacT"
                     /locus_tag="ACHKUB_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO6656010.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         4790 a         4943 c         5262 g         4554 t
ORIGIN      
        1 tttttattct gttgcgattt tggcgttttt taaccataag ctaatgtgat gatcaatttt
       61 accttatggt taacagtctg tttcggtggt aagttcaggc aaaagagaac gattgcgttg
      121 gggaccggga gtggctccga tgctgggttt cgtggtgata atttcaccat gaaaaagttg
      181 tcagccccgc ttattcaatg aggacaagat ggcacaacag actcctttgt acgaacaaca
      241 cacgctttgc ggcgctcgca tggtggattt ccacggctgg atgatgccgc tgcattacgg
      301 ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc ggaatgtttg atgtgtcaca
      361 tatgaccatc gtcgatcttc gcggcagccg cacccgggag tttctgcgtt atctgctggc
      421 gaacgatgtg gcgaagctca ccaaaagcgg caaagccctt tactcgggga tgttgaatgc
      481 ctctggcggt gtgatagatg acctcatcgt ctactacttt actgaagatt tcttccgcct
      541 cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg attacccaac acgctgaacc
      601 tttcggcatc gaaattaccg ttcgtgatga cctttccatg atcgccgtac aagggccgaa
      661 tgcgcaggca aaagctgcca cactgtttaa tgatgctcag cgtcaggcgg tggaagggat
      721 gaaaccgttc tttggcgtgc aggcgggcga tctgtttatt gccaccactg gttataccgg
      781 tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg gccgatttct ggcgtgcgct
      841 ggtggaagcg ggcgttaagc catgcggtct gggcgcgcgt gacacgctgc gtctggaagc
      901 gggcatgaat ctttatggtc aggagatgga cgaaactatt tctcctttag ccgccaacat
      961 gggctggact atcgcctggg aaccggcaga tcgtgacttc atcggtcgtg aagccctgga
     1021 agtgcagcgt gagcatggca cagaaaaact ggttggtctg gtgatgaccg aaaaaggcgt
     1081 gctgcgtaat gaactgccgg tacgttttac cgatgcgcag ggcaaccagc atgaaggcat
     1141 tatcaccagc gggactttct ccccgacgct gggttacagc attgcgctgg cacgcgtgcc
     1201 ggaaggtatt ggcgaaacgg cgattgtgca aattcgcaac cgtgaaatgc cggttaaagt
     1261 gacgaaacct gtttttgtgc gtaacggtaa agccgtcgcg tgatttactt ttttggagat
     1321 tgattgatga gcaacgtacc agcagaactg aaatacagca aagaacacga atggctgcgt
     1381 aaagaagccg acggcactta caccgttggt ataaccgaac atgcccagga gctgttaggc
     1441 gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg ttagcgcggg cgatgactgc
     1501 gcggttgccg aatcagtaaa agcggcgtca gacatttatg cgccagtaag cggtgaaatc
     1561 gtggcggtaa acgacgcact gagcgattcc ccggaactgg tgaacagcga accgtatgca
     1621 ggtggctgga tctttaaaat caaagccagc gatgaaagcg aactggaatc actgctggat
     1681 gcgaccgcat acgaagcatt gttagaagac gagtaacggc tttattcctc ttctgcggga
     1741 gaggatcagg gtgaggaaaa tttatgcctc accctcactc tcttcgtaag gagagaggtt
     1801 cacaattcac tgcacgtttc aggaaccatc gctcatgaca cagacgttaa gccagcttga
     1861 aaacagcggc gcttttattg aacgccatat cggaccggac gccgcgcaac agcaagaaat
     1921 gctgaatgcc gttggtgcac aatcgttaaa cgcgctgacc ggccagattg tgccgaaaga
     1981 tattcagctt gcgaccccgc cgcaggttgg cgcaccggcg accgaatacg ccgcactggc
     2041 agaactcaag gctattgcca gtcgcaataa acgcttcacg tcttacatcg gcatgggtta
     2101 caccgccgtg cagctaccgc cggttatcct gcgtaacatg ctggaaaatc cgggctggta
     2161 taccgcgtac actccgtatc aacctgaagt ctcccagggc cgccttgaag cactgctcaa
     2221 cttccagcag gtaacgctgg atttgactgg actggatatg gcctctgctt ctcttctgga
     2281 cgaggccacc gctgccgccg aagcaatggc gatggcgaaa cgcgtcagca aactgaaaaa
     2341 tgccaaccgc ttcttcgtgg cttccgatgt gcatccgcaa acgctggatg tggtccgtac
     2401 tcgtgccgaa acctttggtt ttgaagtgat tgtcgatgac gcgcaaaaag tgctcgacca
     2461 tcaggacgtc ttcggcgtgc tgttacagca ggttggcaca accggtgaaa tccacgacta
     2521 caccgcgctt attagcgaac tgaaatcacg caaaattgtg gtcagtgtcg ccgccgatat
     2581 tatggcgctg gtgctgttaa ctgcgccggg taaacagggc gcggatattg tttttggttc
     2641 ggcgcaacgc ttcggcgtgc cgatgggcta cggtggccca cacgcggcct tctttgcggc
     2701 gaaagatgaa tacaaacgct caatgccggg ccgtattatc ggtgtatcga aagatgcagc
     2761 tggcaatacc gcgctgcgca tggcgatgca gactcgcgag caacatattc gccgtgagaa
     2821 agcgaactcc aacatttgta cttcccaggt gctgctggca aacatcgcca gcctgtatgc
     2881 cgtttatcac ggcccgattg gcctgaaacg tatcgctaac cgcattcacc gtctgaccga
     2941 tatcctggcg gcgggcctgc aacaaaaagg tctgaaactg cgccatgcgc actatttcga
     3001 caccttgtgt gtggaagtgg ccgacaaagc gggcgtactg acgcgtgccg aagcggctga
     3061 aatcaacctg cgtagcgata ttctgaacgc ggttgggatc acccttgatg aaacaaccac
     3121 gcgtgaaaac gtaatgcagc ttttcaacgt gctgctgggc gataaccacg gcctggacat
     3181 cgacacgctg gacaaagacg tggctcacga cagccgctct atccagcctg cgatgctgcg
     3241 cgacgacgaa atcctcaccc atccggtgtt taatcgctac cacagcgaaa ccgaaatgat
     3301 gcgctatatg cactcgctgg agcgtaaaga tctggcgctg aatcaggcga tgatcccgct
     3361 gggttcctgc accatgaaac tgaacgccgc cgccgagatg atcccaatca cctggccgga
     3421 atttgccgaa ctgcacccgt tttgtccgcc ggaacaggct gaaggttatc agcagatgat
     3481 tgcgcagctg gctgactggc tggtgaaact gaccggttac gacgccgttt gtatgcagcc
     3541 gaactccggc gcacagggcg aatacgcggg cctgctggcg attcgtcatt atcatgaaag
     3601 ccgcaacgaa gggcatcgcg atatctgcct gatcccggct tctgcgcacg gaactaaccc
     3661 cgcttctgca catatggcag gaatgcaggt ggtggttgtg gcgtgtgata aaaacggcaa
     3721 catcgatctg actgatctgc gcgcgaaagc ggaacaggcg ggcgataacc tctcctgtat
     3781 catggtgact tatccttcta cccacggcgt gtatgaagaa acgatccgtg aagtgtgtga
     3841 agtcgtgcat cagttcggcg gtcaggttta ccttgatggc gcgaacatga acgcccaggt
     3901 tggcatcacc tcgccgggct ttattggcgc ggacgtttcg cacctcaacc tgcataaaac
     3961 tttctgcatt ccgcacggcg gtggtggtcc gggtatggga ccgatcggtg tgaaagcgca
     4021 tctggcaccg tttgtaccgg gtcatagcgt ggtgcaaatc gaaggcatgt taacccgtca
     4081 gggcgcagtt tctgcggcac cgttcggtag cgcctctatc ctgccaatca gctggatgta
     4141 catccgcatg atgggcgcag aagggctgaa aaaagcaagc caggtggcaa tcctcaacgc
     4201 taactatatt gccagccgcc tgcaggatgc cttcccggtg ctgtataccg gtcgcgacgg
     4261 tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg aaagaagaaa ccggcatcag
     4321 cgagctggat attgccaagc gcctgatcga ctacggtttc cacgcgccga ccatgtcgtt
     4381 cccggtggcg ggtacgctga tggttgaacc gactgaatct gaaagcaaag tggaactgga
     4441 tcgctttatc gacgcgatgc tggctatccg cgcagaaatc gaccaggtta aagccggtgt
     4501 ctggccgctg gaagataacc cgctggtgaa cgcgccgcac attcagaacg aactggtcgc
     4561 cgagtgggcg catccgtaca gccgtgaagt tgcagtattc ccggcaggtg tggcagacaa
     4621 atactggccg acggtgaaac gtctggatga tgtttacggc gaccgtaacc tgttctgctc
     4681 ctgcgtaccg attagcgaat accagtaatt cactgattcg actatcttct aaaggcgctt
     4741 cggcgccttt ttagtcagat gacaaagtac aaaagcgctc agacagtccc ctcacccctt
     4801 tggggagagg gttagggtga ggggaacagg ccggcactgg cgcgaatatt taccctcacc
     4861 ccggccctct ccctgaaagg gcgaggggaa aagcgtgcca acattgaaga ttgagccagt
     4921 ttgttagcaa tctcaaagat acgtcaacga attaattttt ctcggaaaaa caaatggcta
     4981 tagcacttgt gaccggtggc agtcgcggca tcgggcgggc aactgcatta ctgttggcgc
     5041 aagaagggta tacggtggcg gttaattatc agcaaaacct ccacgcggcg caggaagtga
     5101 tgaacttaat aacgcaagcc ggtggcaaag cattcgtgct ccaggcggat atcagcgacg
     5161 aaaaccaggt cgttgcgatg tttacagcaa tcgatcagca cgatgaaccg ctagcagcgc
     5221 tggtcaataa cgccgggatc ttgtttaccc agtgcaccgt tgaaaacctt acctcagagc
     5281 gaatcaaccg agtactttcc accaacgtga cgggatattt tctctgctgc cgcgaggcgg
     5341 taaaacgcat ggcgcttaaa aatggtggca gtggcggcgc tatcgtcaat gtctcttcgg
     5401 tggcctcacg gttgggttcg ccaggggaat atgttgatta cgcggcatcg aaaggggcga
     5461 ttgatacgtt aaccaccgga ctatcgctgg aagtcgccgc gcaggggatc cgcgttaact
     5521 gcgtgcggcc agggtttatt tataccgaaa tgcacgccag cggcggcgag cctggacgcg
     5581 tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg acaggcagaa gaggtcgcgc
     5641 aggccattgt ctggctacta agtgataaag cctcttacgt cacgggaagt tttatcgatt
     5701 tggcgggcgg gaaataaaac agggaagttg tctgaccgga tgcaacaagt attgcatccg
     5761 gtacttcatc gacttaaagc ttctcgccgt tgctggcaat cacctcttgg taccagttaa
     5821 agctcttctt acgtgaacgc gacatatcgc cagcaccgtc gtcatgttta ttcacataga
     5881 taaagccgta gcgtttgctg tactgcccgg tggtgaacga tacgcagtcg atgcagcccc
     5941 acggtgtgta gcccatcaga tccacgccat cgtaagtcac cgcttttttc atctcttcga
     6001 tatgggcgcg caggtagtca atgcggtagt cgtcgttgat gctgccatct tcttccactt
     6061 tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg cctctgataa cgctcataca
     6121 gttcgcaaag ggcatagcgc agacctactg gatcaatctg ccagccccag tcagacgctt
     6181 taacatacgg gttcggtacg ctgccttcga aaccagagat cgcatcgccg gtgccgcctt
     6241 cggccttcac tgcgttggtc atgtaatagc tgaaaccaag ataatcgcag gtgccttcac
     6301 gcagtacatc gagatcgccg tcttccattt tgatgttaaa tccgcgacgc tcccactcgt
     6361 tcaacacata ggacgggtaa tagccgcgta gctgcacatc ggtaaagacg tagcgttcgc
     6421 gcatcgactc ctgagcgaac atcacatcgt ccgggttaca gcagtaggga tagagcggca
     6481 ccatcgccag catacagccg actttcatct ccgggttaat gcgacgcgca gctttcaccg
     6541 ccagggcgct ggcgacaaac tggtgatgca gcacctggta catcgtctct tccgggttgt
     6601 catgctcggt ataaaccacg ccggagcagc agtaaccgaa cagcggtgca cgccagttac
     6661 gctggttgtt aatttcgttg aaggtcatcc agtatttgac tttatgctta tagcgttcaa
     6721 acaccacttc cgcgaaacgg acaaagaaat caaccacttt acggttggtc cagctaccgt
     6781 attgctgcac cagatgcagc ggcatttcaa agtgggagag ggtgatcacc ggttcgatgt
     6841 tgtatttcag cagttcatcg aacatatcat cgtagaactt cagcccttct tcgtttggct
     6901 gagcttcatc accttttgga aaaatacgcg tccaggcgat ggaagtccga aaacatttga
     6961 agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg accataaaaa tcaacggctt
     7021 catggtttgg atagtatttg cctggcaaga cttctttggt gatttcgcgc ggcacgccgt
     7081 gtgcgccacc ggtcagaacg tcacaaatgc tcggcccttt gccgcctttg ttccagccgc
     7141 cttcgacctg atgagcggca actgcgccgc cccataagaa atctttcggt aaggtgagtt
     7201 ttttcactat cataggctcc agatgagtca attcttacag gaaatagtac catgcagcca
     7261 aacgacatca ctttttttca acgtttccag gatgacattc tggctgggcg taaaaccatc
     7321 accatccgcg acgagtctga atcgcacttc aaaacgggtg atgtgcttcg tgtcgggcgt
     7381 tttgaagatg acggttattt ttgcacgatt gaagtcaccg caacctcaac cgtaacgctg
     7441 gatacgctga cggaaaaaca tgcagagcag gaaaatatga ccctgactga actgataaag
     7501 gtcattgccg acatctatcc cggtcagaca cagttttatg tgattgaatt taaatgtctt
     7561 taaattgaac tcacgcctgt tagttgaagt ttcgcattta atttcatgat ttttaggaga
     7621 ttagtctcaa atcagaattt attttagcta acaggtgttc actggaacta ttctcagtta
     7681 cgctggagag gtatacatgt gcgtgtacgt ttctccggag taagttatgg ttcagaagcc
     7741 cctcattaag cagggatatt cgctggcaga ggaaattgcc aacagcgtca gtcacggcat
     7801 tgggttggtg tttggtatcg ttgggctggt gttgctactg gttcaggcgg tggatctcaa
     7861 tgccagcgcc acagcgataa ccagttacag cctctatggc ggcagtatga tcctgctgtt
     7921 cctcgcttcg acgctctatc acgccattcc tcatcaacgg gcaaaaatgt ggctgaagaa
     7981 atttgaccat tgcgctattt acctgttgat tgccggaacc tacacgccgt ttttgctggt
     8041 ggggctggat tctccgttag cgcgcgggtt gatgattgtt atctggagcc tggcattgct
     8101 gggtattctg tttaaactga ccatcgcgca ccgattcaaa attttatctc tggtgaccta
     8161 tctggcgatg ggctggctgt cgctggtggt aatttatgaa atggcagtta agctcgcggc
     8221 gggcagcgtt accttactgg cggtaggcgg cgtggtttat tcgctcgggg tgattttcta
     8281 cgtctgcaaa cgcattccat acaaccatgc catctggcac ggcttcgtgc tcggcggtag
     8341 tgtgtgccac tttctggcga tctatttgta tattgggcag gcgtaatggg tatgcctttg
     8401 taggcctgat aagacgcgcc agcgtcgcat caggcgataa atgcctgatg cgataaagag
     8461 atttactctt cgagcgaata cggcagcggc tcgatatgca gtgtattcgc atcgtcgcgt
     8521 acgcggaaga tgctatccgg ttccatatcg ttattcatga cgacctgtac cacgacctga
     8581 ccatcttcca gttttaccgc agccagcacc gtaccggtac gacgccagtt ctcgcccatt
     8641 ttcagctcta agtcttcacc agcttccggc agtcggctgg cgctacctgc cagcaaccag
     8701 agcgcacgtt tattggcacc acggaatttt gctcgcgcca ccatctcttg tccggtatag
     8761 cagccttttt taaagctgat gccgcccagc gcctggaggt tggtcgcctg tgggataaac
     8821 tgcccgctgt tggcggcatc aatcaccggg aaacccgctt caatgtttaa tgccagccac
     8881 tgttggctat tgttcagttc cgcttcaccg cgcagtttat cggtcagcat attagcagta
     8941 gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt caaaccatag caaagtggtc
     9001 gcgccttctt taactacctg tttttctttc gaaggcagtt cactaaagag atttgccagc
     9061 gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac gctcgtcgtc tggcgcgatg
     9121 gtcactttag agaataccgc atattttttc agttcagtca gctgcggttc acgcacgctg
     9181 cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca gacgtaaatt gctccacatt
     9241 ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat cttctgccat ctggctgaca
     9301 tctgctgtca cctgaccctg catatatttt tcgctgtccg cgccagtaat ggtggcaagc
     9361 gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg cagaagccgt aggctgacgg
     9421 ggaggaaaag gtgtaaaagc cataatagag tcctgagttg cttaacggcg tattaatgac
     9481 taatggtaaa agagctatga ggcaatgcaa gtgctttcac tacgcgatct gtgccttctg
     9541 cgaggcgact tccaaaaaaa tgaagccatt gaattgttgt gaattaattt cgcgatcgcg
     9601 atggtgttta ctgatattcc cgtaaaagaa ggcgaaaaac acgttacaat agccgggtac
     9661 tggattttcg tgagacagga agaagatgga cattaacaac aaagcccgca ttcattgggc
     9721 atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg ccgtttttcg aacatgagta
     9781 cgacagctta agcgatgacg aaaaacgcat ctttattcgt ctgctggaat gtgacgatcc
     9841 ggacctgttt aactggctga tgaatcacgg taaaccggcc gatgcagaac tggaaatgat
     9901 ggtccgactc atccagacac ggaaccggga acgtggtcct gtggcaatct gatttgcgcg
     9961 tctcctggcg cgcacagtgg ctttccttgc tgattcatgg gctggttgcc gctgttattt
    10021 tactcatgcc ctggccactc agttacaccc cgttatggat ggtgttactt tcgctggtgg
    10081 tgtttgattg cgttcgcagc cagcggcgta ttaatgctcg ccagggggaa attcgcttgt
    10141 tgatggacgg gcgtttgcgt tggcaagggc aggagtggtg catcgtcaaa gcaccgtgga
    10201 tgattaagag cggcatgatg ctgcgtttac gttctgatgg cggtaaacgg caacatttat
    10261 ggctggcagc cgacagcatg gacgaagccg aatggcggga tttacggcgg attttgttgc
    10321 aacaagagac gcaaagataa agatgacggc aaagcatatg cgctaagccg tcagtgagat
    10381 caggcgtaat gctctgccat ttcgttgagg atttgctcgc accagctctg aatacgctcg
    10441 tcgctaaggt catactggtt agtttcatcc agcgccagac ccacgaacag ttgcccgtca
    10501 gcaatcaccg gtttcgggct ggtaaattca tatccttccg ttggccagta gccgacgaac
    10561 ttcacgcctt tggtcgagag tttgtcatgc agcataccga gcgcatcgag gaaccactcg
    10621 ccgtatccca gttgatcgcc aagcccatac agcgcaacaa ttttaccttc aaggttcagg
    10681 tcgtcgagct gatcccagac ggcttcccag tcttcctgga tttcaccaaa atcccaggtc
    10741 gggataccca gaatgagcac atcgtactgc tccattaatt tcggggagtc gtccttgagg
    10801 ttatgtaagg tcaccagttc tgggccgata atatcgcgga ttttttctgc cgccatttcg
    10861 gtgtaacagg tgctggaacc gtaaaaaaga cccatattca taacgtaagc ttctcgattc
    10921 tgctgtggct tttgcagcta gtgtatcaga atcgctttag tcacggcaca ataaggcata
    10981 atggatcaaa tgagtgacca taaggggcgc aagtgaaaca ggatctggca cgcatcgagc
    11041 agtttcttga tgctctgtgg ctggaaaaaa atctggctga aaatacgttg aacgcttacc
    11101 gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg cgggttgacg ctggcgacgg
    11161 cgcaaagtga cgatttgcag gcattactgg cagaacggct ggagggcggg tataaagcca
    11221 ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt ccagtatctt tatcgcgaaa
    11281 agtttcgtga agacgatccc agtgcacatc tcgcttcacc gaaattgccc cagcgtttgc
    11341 caaaagattt aagcgaagcg caggtcgaac gtttattaca ggcaccatta attgatcagc
    11401 cactggagct acgcgataaa gccatgcttg aagtgttgta tgctaccggg ctgcgtgtct
    11461 ctgaactggt cggactgaca atgagtgata tcagcctgcg tcagggcgta gtgcgggtca
    11521 ttggtaaagg caacaaagag cgcctggtac cgttaggtga agaggcggtt tactggctgg
    11581 aaacctatct ggaacatggg cgtccgtggc tgctgaatgg tgtgtcaatt gacgtgttgt
    11641 ttcccagcca gcgtgcgcag cagatgacgc gacagacctt ctggcaccgc attaaacatt
    11701 atgctgtgct ggcgggtatc gacagcgaaa agctgtcacc gcatgtattg cgtcacgctt
    11761 ttgccaccca tttattaaat catggtgcgg atttacgcgt ggtgcagatg ctactgggcc
    11821 acagcgacct ctccaccacg caaatttata cgcatgtcgc taccgagcgt ctgcggcaac
    11881 ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag aacgggaaga tttatgaaga
    11941 aaggttttat gttgtttact ttgttagcgg cgttttcagg ttttgctcag gctgatgacg
    12001 cggcaattca acaaacgtta gccaaaatgg gcatcaaaag cagcgatatt cagcccgcgc
    12061 ccgtagctgg catgaagaca gttctgacta acagcggcgt gttgtacatc accgatgatg
    12121 gtaaacatat cattcagggg ccaatgtatg acgttagcgg cacggctccg gtcaatgtca
    12181 ccaataagat gctgttaaag cagttgaatg cgcttgaaaa agagatgatc gtttataaag
    12241 cgccgcagga aaaacacgtc atcaccgtgt ttactgatat tacctgtggt tactgccaca
    12301 aactgcatga gcaaatggca gactataacg cgctggggat caccgtgcgt tatcttgctt
    12361 tcccgcgcca ggggctggac agcgatgcag agaaagaaat gaaagctatc tggtgtgcga
    12421 aagataaaaa caaagcgttt gatgatgtga tggcaggtaa aagcgtcgca ccagccagct
    12481 gcgacgtgga tattgccgac cattacgcac ttggcgtcca gcttggcgtt agcggtactc
    12541 cggcagttgt gctgagcaat ggcacacttg ttccgggtta ccagccgccg aaagagatga
    12601 aagaattcct cgacgaacac caaaaaatga ccagcggtaa ataattcgcg tgaaacaaca
    12661 gatacaactt cgtcgccgtg aagtcgatga aacggcagac ttgcccgctg aattgcctcc
    12721 cttgctgcgc cgtttatacg ccagccgggg cgtgcgcagt gcgcaagaac tggaacgcag
    12781 tgttaaaggt atgttgccct ggcagcaact gagcggcgtc gaaaaggccg ttgagatcct
    12841 ttacaacgct tttcgcgaag gaacgcggat tattgtggtc ggcgattttg acgccgacgg
    12901 cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg cttggttgca gcaatatcga
    12961 ctatctggta ccaaaccgtt tcgaagacgg ttacggctta agcccggaag tagtcgatca
    13021 ggcccatgcc cgtggcgcgc agttaattgt cacggtggat aacggtattt cctcccatgc
    13081 gggcgttgaa cacgctcgct cgttgggcat tccggttatt gttaccgatc accatttgcc
    13141 gggcgaaaca ttacccgcag cggaagcgat cattaaccct aacttgcgcg actgtaattt
    13201 cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat ctgatgctgg cgctgcgcac
    13261 ctttttgcgc gatcagggct ggtttgatga gcgcggcatc gcaattccta acctggcaga
    13321 actgctggat ctggtagcgc tggggacagt agcggacgtc gtgccgctgg acgctaataa
    13381 tcgcattctg acctggcagg ggatgagtcg catccgtgcc ggaaagtgcc gtccggggat
    13441 taaagcgctg ctggaagtgg caaatcgcga tgcgcaaaaa ctcgccgcca gcgatttagg
    13501 ttttgcgctg gggccacgtc tcaatgctgc cgggcgactg gacgatatgt ccgtcggtgt
    13561 ggcgctcttg ctgtgcgaca acatcggcga agcgcgcgtg ctggcaaatg aactcgatgc
    13621 gctaaaccag acgcgaaaag agatcgaaca aggaatgcaa gttgaagccc tgaccctgtg
    13681 cgagaaactg gagcgaagtc gcgacacgct acccggcggg ctggcaatgt atcaccccga
    13741 atggcatcag ggcgttgtcg gtattctggc ttcgcgcatc aaagagcgtt ttcaccgtcc
    13801 ggttatcgcc tttgcgccag caggtgatgg tacgctgaaa ggttccggtc gctccattca
    13861 ggggctgcat atgcgtgatg cactggagcg attagacaca ctctaccctg gcatgatgct
    13921 gaagtttggc ggtcatgcga tggcggcggg tttgtcgctg gaagaggata aattcgaact
    13981 ctttcaacaa cggtttggcg aactggttac tgagtggctg gacccttcgc tattgcaagg
    14041 cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg accatggaag tggcgcagct
    14101 gctgcgcgat gctggcccgt gggggcagat gttcccggag ccgctgtttg acggtcattt
    14161 ccgtctgctg caacagcggc tggtgggcga acgtcatttg aaggtgatgg tcgaaccggt
    14221 cggcggcggt ccactgctgg atggtattgc ttttaatgtc gataccgctc tctggccgga
    14281 taacggcgtg cgcgaagtgc aactggctta caagctcgat atcaacgagt ttcgcggcaa
    14341 ccgcagcctg caaattatca tcgacaatat ctggccaatt tagcgtcatc ttctctataa
    14401 aaaagagcgt ggattgggta caatcccgct cttatcaccg cattttgact agctcaataa
    14461 aagaaatcag accatgtttg aaattaatcc ggtaaataat cgcattcagg acctcacgga
    14521 acgctccgac gttcttaggg ggtatctttg actacgacgc caagaaagag cgtctggaag
    14581 aagtaaacgc cgagctggaa cagccggatg tctggaacga acccgaacgc gcacaggcgc
    14641 tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac cctcgaccaa atgaaacagg
    14701 ggctggaaga tgtttctggt ctgctggaac tggctgtaga agctgacgac gaagaaacct
    14761 ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa actggcgcag cttgagttcc
    14821 gccgtatgtt ctctggcgaa tatgacagcg ccgactgcta cctcgatatt caggcggggt
    14881 ctggcggtac ggaagcacag gactgggcga gcatgcttga gcgtatgtat ctgcgctggg
    14941 cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc ggaaggtgaa gtggcgggta
    15001 ttaaatccgt gacgatcaaa atctccggcg attacgctta cggctggctg cgtacagaaa
    15061 ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc cggcggtcgt cgccacacat
    15121 cgttcagctc tgcgtttgtt tacccggaag ttgatgatga tattgatatc gaaatcaacc
    15181 cggcggatct gcgcattgac gtttatcgcg cgtccggcgc gggcggtcag cacgttaacc
    15241 gtaccgaatc tgcggtgcgt attacccaca tcccaaccgg gatcgtgacc caatgccaga
    15301 acgaccgttc ccagcacaag aacaaagacc aggccatgaa gcagatgaaa gcgaagcttt
    15361 atgaactgga gatgcagaag aaaaatgccg agaaacaggc gatggaagat aacaaatccg
    15421 acatcggctg gggcagccag attcgttctt atgtccttga tgactcccgc attaaagatc
    15481 tgcgtaccgg ggtagaaacc cgcaacacgc aggccgtgct ggacggcagc ctggatcaat
    15541 ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa catgtctgaa caacacgcac
    15601 agggcgctga cgcggtagtc gatcttaaca atgaactgaa aacgcgtcgt gagaagctgg
    15661 cgaacctgcg cgagcagggg attgccttcc cgaacgattt ccgtcgcgat catacctctg
    15721 accaattgca cgcagaattc gacggtaaag agaacgaaga actggaagcg ctgaacatcg
    15781 aagtcgccgt tgctggccgc atgatgaccc gtcgtattat gggtaaagcg tctttcgtta
    15841 ccctgcagga cgttggcggt cgcattcagc tgtacgttgc ccgtgacgat ctgccggaag
    15901 gcgtttacaa cgagcagttc aaaaaatggg acctcggcga catcctcggc gcgaaaggta
    15961 aactgttcaa aaccaaaacc ggcgaactgt ctatccactg taccgagctg cgtctgctga
    16021 ccaaagcact gcgtccgctg ccggataaat tccacggctt gcaggatcag gaagcgcgct
    16081 atcgtcagcg ttatttggat ctcatctcta acgatgaatc ccgtaacacc tttaaagtac
    16141 gctcgcagat cctctctggt attcgccagt tcatggtgaa ccgcggcttt atggaagttg
    16201 aaacgccgat gatgcaggtg atccctggcg gtgccgctgc gcgtccgttt atcacccacc
    16261 ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc ggaactgtac ctcaagcgtc
    16321 tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg taacttccgt aacgaaggta
    16381 tctccgtacg tcataaccca gagttcacca tgatggaact ctacatggct tacgcagatt
    16441 acaaagatct gatcgagctg accgaatcgc tgttccgtac tctggcacag gatattctcg
    16501 gtaagacgga agtgacctac ggcgacgtga cgctggactt cggtaaaccg ttcgaaaaac
    16561 tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac cgacatggcg gatctggata
    16621 acttcgactc tgcgaaagcg attgctgaat ctatcggtat ccacgttgag aagagctggg
    16681 gtctgggccg tatcgttacc gagatcttcg aagaagtggc agaagcacat ctgattcagc
    16741 cgaccttcat tactgaatat ccggcagaag tttctccgct ggcgcgtcgt aacgacatta
    16801 acccggaaat cacagaccgc tttgagttct tcatcggtgg tcgtgaaatc ggtaacggct
    16861 ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt cctggatcag gttgccgcga
    16921 aagacgcagg tgacgacgaa gcgatgttct acgatgaaga ttacgttacc gcactggaac
    16981 atggcttacc gccgacagca ggtctgggga ttggcatcga ccgtatggta atgctgttca
    17041 ccaacagcca taccatccgc gacgttattc tgttcccggc gatgcgtccg gtaaaataag
    17101 cattacgtta tgctcacaac cccggcaaat gtcggggttt tttaattgtg ctgcgcgaaa
    17161 gcagacaaca attttcttgc ttcactattg gctgcctgca tcaccatcca cggactgaac
    17221 gcccacggtg tggcatcaat accgtgtaat acatctgcta aatcacacca ttgataatcc
    17281 atcacttcat catcgttgat ctgtaacgca ctggtggtgc gtgcggcaaa taccggacac
    17341 acttcatttt ccacaatgcc attcggatcg gtggcgcgat agcgaaagtc aggatagata
    17401 gattcaggag gcgtaatttc cacgccaagc tcataacggc aacggcggat caccgcgtct
    17461 tcgttgcttt ctcccagttg tgggtgccca caaaccgagt tagtccacac gccaggccac
    17521 gcttttttgc tcagtgcgcg gcgggtaact aataattgtc ctttggcatt aaacagccaa
    17581 ctggagaacg cgagatgtaa gcgggtgtct gccgtgtgtg cggcatactt ttccagcgta
    17641 cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt ccgtttgcat aatttctcac
    17701 atgtaattct gatcactcat cattatagat aattgaagag tgaaatgatt aaccaagtga
    17761 gcgtttatcg acaaccgccc gttttgagcg gatgccgaca ggtaaaaacc atttaaaaaa
    17821 gtagcatcat atatttgaag cgactttcaa tggccattgc aacacagtca gaaatgacag
    17881 gaatagagag agtaaaaagc aacagcagac tgaatatagc gcgcattaaa aaattcatat
    17941 tattctccat gctcattttt cttcagcttg cgcatcagaa gccacatacg tactgtcctg
    18001 acttttaaat ctttatcaga gacggattcc ttcaccaggt cagcatgatg ctggtagcca
    18061 ttaaagaaga cattcagcac aacggcgcta agggttgcga gcataatgcc gctatgcagc
    18121 aacggttgca gtacggccgg taacttagaa aagaaatcgt gagagagcgt cggagtcatg
    18181 ccaacgccga gactgatcgc cacaatatag aggttgtaac ggttggtggt gtagttacag
    18241 cgcgacagaa ttcgaatccc tgtcgccagt accatgccga acatcaccag cccagcaccg
    18301 cccagcacaa attgcggaat ggaggcgacc agcaccgcca tttttggcac catgccaaac
    18361 aggattaata taattcccga agaaatacac acccagcggc tatgaacgcg cgtcacgcta
    18421 accaggccaa cgttttgtga aaatgacgtg tgggggaagc tgttaaacgt tccgcctatc
    18481 attgtcccta cgccatcgac acgcagcccg cgaataatat cgtgtgaaga gagtttacga
    18541 ccgactattt cacccagtgc caggaacatc cccatcgact cgataaacac gatgattaac
    18601 acggcagtca tggtcagaat ggatacggga tcgaaaatcg gcataccaaa tgacatcggc
    18661 gtgacaatcg caaaccatga agcatcatgt agcccggata aattgacttc attcatcatc
    18721 cacgaaagta aaaagccaaa tacaatcccc agtaatacgg cgacgttgga cataaatcct
    18781 ttcgcatagc gagtaatgag caagataaaa attaagacag caaaggagat acctaaataa
    18841 acgggattac catattgcgg attcccttta cctccggcgg cccagtcaat acccacctga
    18901 atgatgctaa gcccaataga agtaataacc acaccggtaa ccagtggcgg gaataaaggc
    18961 atcaagcgac caataagtgg cgctaataat gtggtgataa aacccgcggc gatagtggca
    19021 ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa tggctatcat tggtgttaca
    19081 gcagcaaagg tcaccgacat aatcaccggc aggcggatcc ccataaagcg gccgataccg
    19141 atacattgca ataatgtgac gatcccgcag caaaagagat ccgagctaat gagcatcgca
    19201 atagcttctt ttgagaggcc cagtcggtca ccaatcataa gaggaacagc gactgcacct
    19261 gcgtacatga caaggacgtg ttgcagaccg agtatgatca gctttcctgg tgataatatg
    19321 cggtcaacct catcagttgg gcggtcttgc ccagaagatg agggaagttg ggaatctatg
    19381 gcgctcatca gtgtttctcc tttcgcccgc cagagtaaaa agcaaccgtt tacgttaatg
    19441 aaactgtcta aacacatgct cgtggagtgc cagtttttgc gaggaagggg agttagagac
    19501 aggaattgcg gaggcaatca cataatcgtg gttaaaccac gaaaatgag
//
LOCUS       DAZFRN010000088        19415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFRN010000088 DAZFRN010000000
VERSION     DAZFRN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.088"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..677)
                     /locus_tag="ACHKUB_003028"
     CDS             complement(<1..677)
                     /locus_tag="ACHKUB_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6656011.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFS"
     gene            685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKUB_003029"
                     /pseudo
     CDS             685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKUB_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1174..1467)
                     /locus_tag="ACHKUB_003030"
                     /pseudo
     CDS             complement(1174..1467)
                     /locus_tag="ACHKUB_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            1739..2533
                     /gene="yjfZ"
                     /locus_tag="ACHKUB_003031"
     CDS             1739..2533
                     /gene="yjfZ"
                     /locus_tag="ACHKUB_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO6656012.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(2604..3053)
                     /gene="rplI"
                     /locus_tag="ACHKUB_003032"
     CDS             complement(2604..3053)
                     /gene="rplI"
                     /locus_tag="ACHKUB_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO6656013.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(3095..3322)
                     /gene="rpsR"
                     /locus_tag="ACHKUB_003033"
     CDS             complement(3095..3322)
                     /gene="rpsR"
                     /locus_tag="ACHKUB_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO6656014.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(3327..3641)
                     /gene="priB"
                     /locus_tag="ACHKUB_003034"
     CDS             complement(3327..3641)
                     /gene="priB"
                     /locus_tag="ACHKUB_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO6656015.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3648..4043)
                     /gene="rpsF"
                     /locus_tag="ACHKUB_003035"
     CDS             complement(3648..4043)
                     /gene="rpsF"
                     /locus_tag="ACHKUB_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO6656016.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4370..4645
                     /gene="yjfY"
                     /locus_tag="ACHKUB_003036"
     CDS             4370..4645
                     /gene="yjfY"
                     /locus_tag="ACHKUB_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO6656017.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4720..5271)
                     /locus_tag="ACHKUB_003037"
     CDS             complement(4720..5271)
                     /locus_tag="ACHKUB_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO6656018.1"
                     /translation="MAIKGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCVV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5366..6052)
                     /gene="ulaF"
                     /locus_tag="ACHKUB_003038"
     CDS             complement(5366..6052)
                     /gene="ulaF"
                     /locus_tag="ACHKUB_003038"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO6656019.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(6052..6906)
                     /gene="ulaE"
                     /locus_tag="ACHKUB_003039"
     CDS             complement(6052..6906)
                     /gene="ulaE"
                     /locus_tag="ACHKUB_003039"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO6656020.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(6916..7566)
                     /gene="ulaD"
                     /locus_tag="ACHKUB_003040"
     CDS             complement(6916..7566)
                     /gene="ulaD"
                     /locus_tag="ACHKUB_003040"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6656021.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(7580..8044)
                     /gene="ulaC"
                     /locus_tag="ACHKUB_003041"
     CDS             complement(7580..8044)
                     /gene="ulaC"
                     /locus_tag="ACHKUB_003041"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO6656022.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(8054..8359)
                     /gene="ulaB"
                     /locus_tag="ACHKUB_003042"
     CDS             complement(8054..8359)
                     /gene="ulaB"
                     /locus_tag="ACHKUB_003042"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO6656023.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(8375..9772)
                     /gene="ulaA"
                     /locus_tag="ACHKUB_003043"
     CDS             complement(8375..9772)
                     /gene="ulaA"
                     /locus_tag="ACHKUB_003043"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO6656024.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            10127..11191
                     /gene="ulaG"
                     /locus_tag="ACHKUB_003044"
     CDS             10127..11191
                     /gene="ulaG"
                     /locus_tag="ACHKUB_003044"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO6656025.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            11299..12054
                     /gene="ulaR"
                     /locus_tag="ACHKUB_003045"
     CDS             11299..12054
                     /gene="ulaR"
                     /locus_tag="ACHKUB_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO6656026.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQMVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(12051..12800)
                     /gene="yjfP"
                     /locus_tag="ACHKUB_003046"
     CDS             complement(12051..12800)
                     /gene="yjfP"
                     /locus_tag="ACHKUB_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6656027.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            12982..13311
                     /gene="bsmA"
                     /locus_tag="ACHKUB_003047"
     CDS             12982..13311
                     /gene="bsmA"
                     /locus_tag="ACHKUB_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO6656028.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            13460..13735
                     /gene="yjfN"
                     /locus_tag="ACHKUB_003048"
     CDS             13460..13735
                     /gene="yjfN"
                     /locus_tag="ACHKUB_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO6656029.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(13852..15477)
                     /gene="aidB"
                     /locus_tag="ACHKUB_003049"
     CDS             complement(13852..15477)
                     /gene="aidB"
                     /locus_tag="ACHKUB_003049"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO6656030.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(15561..16724)
                     /gene="yjfC"
                     /locus_tag="ACHKUB_003050"
     CDS             complement(15561..16724)
                     /gene="yjfC"
                     /locus_tag="ACHKUB_003050"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6656031.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESGAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(16727..17365)
                     /gene="yjfM"
                     /locus_tag="ACHKUB_003051"
     CDS             complement(16727..17365)
                     /gene="yjfM"
                     /locus_tag="ACHKUB_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO6656032.1"
                     /translation="MARKRKSRNNSKFGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(17375..17773)
                     /gene="yjfL"
                     /locus_tag="ACHKUB_003052"
     CDS             complement(17375..17773)
                     /gene="yjfL"
                     /locus_tag="ACHKUB_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO6656033.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(17791..18450)
                     /gene="yjfK"
                     /locus_tag="ACHKUB_003053"
     CDS             complement(17791..18450)
                     /gene="yjfK"
                     /locus_tag="ACHKUB_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO6656034.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(18501..19199)
                     /gene="yjfJ"
                     /locus_tag="ACHKUB_003054"
                     /pseudo
     CDS             complement(18501..19199)
                     /gene="yjfJ"
                     /locus_tag="ACHKUB_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
     gene            complement(19218..>19415)
                     /locus_tag="ACHKUB_003055"
     CDS             complement(19218..>19415)
                     /locus_tag="ACHKUB_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO6656035.1"
                     /translation="NTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILL
                     EITSLVDNALDLAEITEEYSH"
BASE COUNT         4972 a         5136 c         4599 g         4708 t
ORIGIN      
        1 ttgctgaatt tgaccctgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgac cattgatgct gaagggtgaa ccggagtgga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
      241 actcccgtga cctgacggct attccggtag cgcatccagc caagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat
      661 gcctgtagtc gttccacagg tcaaattata ttttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
      781 cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccaat agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cagataaacc aggccaagcg tatgctggtt
     1201 gatcaaataa tatcccccaa gcgcagcaat attaatattc acctgcaatg ggtcgatacc
     1261 tggcttaaaa atattgtatt tcttcccttc atccaataag gattccatta actgcaagtg
     1321 cgcatggtta atttctaaga gacgctgtga ttttgcataa tgcacacctt tactttgatt
     1381 ttcactatgc acaattttta aaaaccaagg gttagcaata taataatccc atgtaaaatc
     1441 aataattttc tcaatggctt tttgtggctg gttaaccaga tgattaacaa acgcaccgat
     1501 accaacagcg tagaataatt acataccgca caacaagaat gtatggtgga aggcgtggaa
     1561 gtcattttct ccccgggggc atcgttatat ccataatgag cgatttttga tattaatatc
     1621 tttcaccatc cgctattttc actacttcag aaaacaaact tatttttatt aatatcgatt
     1681 ctttttctgt gaacatcagc tgcgatattt ataaaaacat taaacaggta acattacaat
     1741 gacattacca acgactattt attcatttcc tgcatattca ccccattttt cgtccaccga
     1801 caaaccagta aaattaaaat ttcatcaata tgcacgtgct acattactgc caaatcgtgg
     1861 aagagatcat aattgcgatg gcagacgtac tgttgagatc cacaaactag atctctctga
     1921 ctggcaagca ttcaataagt tagcaacgaa gtgtaatgct tacgatggag taacaatgaa
     1981 tggtgataat tcttttggat ggaaccatga agctacatta gataatattc acgcccagaa
     2041 atacaataaa gcttacgcgg gtgcgaggtt aaccgcagaa ctgaaatatt tattgcaaga
     2101 tgtcgaatcc ttcgaaccca acagtaaata cacaatacac gaggttgttt tgggacctgg
     2161 ttatagtact ccagactata ccggccaaac gataggatac gttgtaaccc ttcctgccca
     2221 aatgcctaat tgctggtcat ctgaactacc aacaatcgat ctttatatcg accaacttcg
     2281 cactgttaca ggcgtttcta atgccctcgg attcatcatt gctgcattgc taaacgcgta
     2341 ttctgattta ccccatgatt tgaaaattgg tctacgttcc ttatcaaatt cagcagcaat
     2401 ctactcgggt ttaggtttcg agcgagtgcc gcaggaccga gacataagga gtgatcgtat
     2461 gcatctaacg ccagcaaacc atcctgactt atggacacaa gagaatggtg aatggatcta
     2521 cttgcgaaac taatacctga cgaaaaatag aaagcaaaac gccgaccaat ggtcggcgtt
     2581 tttacgtctc gttgaataac gaattattca gctactacgt ttacgatcac tttcgcgaat
     2641 acttcgctgt gaacctggaa gctcacttcg tgttcgccag tggtacgcag aacgccgttc
     2701 ggcagacgaa cttcgctctt agccacttca acgccagctg cagttacagc gtcagcgatg
     2761 tcgcgagtac cgatggaacc gaacagttta ccttcgtcgc cagctttaga cgcgatggta
     2821 acagtttcca gtgcattgat tttctcagcg cgagcattag cagctgccag aacttcagcc
     2881 agtttagctt ccagttcagc gcgacgtgct tcgaagaatt caatgttttt cttggtagct
     2941 ggaacagctt taccctgcgg taccaggaag ttacgagcat agcccgcttt aacgtttacc
     3001 tgatcaccca ggctacccag gtttgctact ttatcaagca gaataacttg cattacctta
     3061 tcctctcaaa gtcgtattaa tggaccgtga ccgattactg atggcgatca gtgtacggca
     3121 gcagggacag gtagcgagcg cgtttgatag cgcgagccag ctgacgctgg tattttgcac
     3181 gggtaccggt gatacggctt gggacaatct taccgctttc ggtgatgtag tttttcagcg
     3241 tagcgatatc tttatagtcg atctcttgaa cgccttccgc ggtgaaacgg cagaacttgc
     3301 gacgacggaa ataacgtgcc atatggctag tctccagaat ctatcaattc aatctgctcg
     3361 gcatgcaaaa ccattttgct cagtccgttc tttgccttgt ggcatgaaat gaacccctga
     3421 acggttatgc gactgccgac cgttatactg tgagtaatgg cctggttttc gtgtccgcta
     3481 acaataacgg gcatttggca ccacgcctgc cggtgaaaac cggcttcctc ctgcacagaa
     3541 cgatgctcaa gcacgaactg gcagtgagga attcctgatg gactgacctt tcgaagggga
     3601 gtcctgcaca cggtgccgga caacaccaga cggttggtca tcagaaatta ctcttcagaa
     3661 tccccagctt cagcatcatc agcggtttcg tttgcgaaat catcgcgacg ctcacggcgc
     3721 tcgtctttcg ctttaaccat cggagatgct tcggtaacag cgtgcttggt acgcataacc
     3781 atgctgcgga taacggcatc gttgaagcgg aaggtagttt ccagctcatc gatcacttcc
     3841 tgcggagctt caacgttcat cagaacgtag tgtgctttgt gcagtttgtt gatcgggtaa
     3901 gccagctgac ggcggcccca gtcttccaga cggtggatct tgccttctgc accagtgatg
     3961 gcagcagtgt agcgctcgat catgcccgga acctgttcgc tctggtcagg atggaccata
     4021 aaaacgattt cgtaatgacg catcgaattg ctccttacgg attattcagc ctcctgtctg
     4081 ggtcagccga atcccgggga ggcaaggaac gtgttaaagg tcggctgaaa aatgacgcgt
     4141 tatagtactt gtatgccccg ctaaactcaa ggccagccga caaataaatc tcatctttcg
     4201 ctcaaaagaa ctaactccct gatttgaagt tcatcacatt ccgtcacagt gttttttcga
     4261 acaactgcat cagaaatggt caccatcaac ttctttatca ttgattaaaa ttaaatcaga
     4321 agatcatcac agaggagagc acacaaagtc agccgtcgag gaggtatcta tgttcagtcg
     4381 agttttagcc cttctggctg tgcttttgct aagtgcaaat acatgggcag ccattgaaat
     4441 taataaccac caggccagaa acatggatga tgtgcaaagc ttaggcgtga tttatatcaa
     4501 tcataatttc gccactgaaa gcgaagcgcg tcaggcatta aatgaagaga cagatgcgca
     4561 gggcgcaacg tactaccacg taattctgat gcgggaaccg gggagtaacg gcaatatgca
     4621 cgccagcgcg gatatttatc gctagcacca ggtataacca acgaaacatt gccatagttt
     4681 gctttgcccc cttcgcaggg ggctttttta tacggttatt caccgaattg cgacttccgg
     4741 atctggctca tgagtaatgc gattacgtaa gtcgcggcgc ataatctcta ttatccagaa
     4801 ccagaaaata tgccccagga tttcagacgc atattcgtca aaaggcaatt gagtgagcgg
     4861 cggcgttaaa ttcagcgccg ggcaaaatat accgtggact gccactgtca caataagccc
     4921 tgctaatatc ccctgccata atttcacttt cggaaaaatt tccgcgacaa cacaataccc
     4981 tatcgcaaaa accagtgaga agatgatatg cgtcaccatc accgggttga taatatgttc
     5041 cgagaatgtg tacacagttt gggttgggtc gatacctaaa taatctcgta gaaagatata
     5101 cggcgggtta aattcatcgc gaccaccgga aaatgtgcgc ggtggcaagg ggacttccgc
     5161 tccccattta acaaatgctg aaataatgcc tgaaagtaca cccacaagta acgcaacgcc
     5221 atagcggcga cgctgcggat ccgttctgac aaacaatcct ttgatagcca taaagcacct
     5281 gatgttgatg attttattaa ctggagaggc cccggaatca gaccaattac atcccgcagg
     5341 aggtaataat tccggggcgc actgcttact tctgcccgta ataagcgtta ggaccgtgtt
     5401 tacgcatgaa gtgtttattc atgaggtagc tgtcgatgtg attcagttgt gggttaatgc
     5461 cgcgggcaat ccacgccatt ttcgccactt cttccatcac caccgcgtta tgcaccgcat
     5521 cgtgagcatc tttcccccag gcgaacggcc cgtgctgata caccacaatt cccggcgtat
     5581 gcagcggctc ggcgttgccc agcgtttcga taatcacttt gccggtgttc agttcatact
     5641 cgccctgcac ctcttcttcg cttaacccgc gcgtacacgg aatgtcgcca aagaagtagt
     5701 cggcgtgcgt ggtgcctaac gccgggatcg ccagccccgc ctgcgcccat gcggtggcat
     5761 gagtggagtg ggtatggaca atgccgccaa gcgacgggta acgacggtag agttcaagat
     5821 gcgtcgcggt gtcggaagac ggacgatatt ccccttccac caccttaccg ctcatatcga
     5881 ccaccaccat atcgtctgct ttcatggttt cgtaggcaac gccgctgggc ttgatcacca
     5941 ccagcccgcg ttcgcggtcg atagcgctga cgttgcccca ggtaaaggtc accagcccgt
     6001 agcgcggcag ctccatgttg gcttcaaata cctgctgttt tagcttttgc attatgccgc
     6061 ctccaccatg cccgctttcg ccatgcgcgc tttcacccaa tcacgcgctt tcgccacttc
     6121 tgccgccggg tcttccgccg tttcgctcca catctcaatc aggtacggcc cgcaatagcc
     6181 actctgtttt agcgtttcga aacaacgttc gaaatccact acaccttcgc caaacggcac
     6241 gtttttgaag acgcccggtt tggtgtcttt cacatgcacc gcgacgatat gcccgattcc
     6301 ggcctgcaac tccatctgca cgtcgttatc ccacgccgac aggttgccga tatccgggta
     6361 gagctggaac cacggattgt tgagatagtg cgcgtagccc agcgccttgc tgatggagtt
     6421 catcaacgga taatccatga tctccatcgc cagcgtcacc tgcgcgcggc ttgccatctc
     6481 aacgctctct ttcaggccgt cacggaaacg acgacgcgtt tcgttattgg cttcctgata
     6541 gtaaacgtca tagcccgcca gctggatcac gcgaataccg acatcctggg cgaactggat
     6601 agctttacgc ataatctcca gcccctgcgc ccgcaccgcg tcatcttcac tacccagcgg
     6661 gaaacgacga tgagcagaaa ggcacatgga cggcacgcgc acgccggttt caacaatcgc
     6721 attgaccagc gccagacgct gctcgcggct ccagtcgagg cgcgacaggc gctcgtccgt
     6781 ttcatctacc gacatctcga caaaatcgaa gcccaacgtt tttgccagtt gcaggcgttc
     6841 cagccagcac tccccggcgg ggagcgcttt ttcatagatg ccaagcggga tttgtttgga
     6901 caacatatcc gctccttagc cccacagttc agcgatggaa cgtttgaact gacgtgcggc
     6961 ttccaccgga gaagcggcat cacggatact acggcccgcg ataaagacgt gaatcggaat
     7021 acccttgaac agcggcagat cttccagcgc caggccaccg gtgacggtga ctttgaagcc
     7081 catatcggaa agacgtttga tcgcggtaat gtccgcttcg ccccacgcta cgcctgcggc
     7141 ctgcgcgtca cggctgcggt gataaaccac ctgctgaatg cctgcgtcac gccactgctg
     7201 cgcctgttcc caggtccagt agccggtcag ttcgatctgc acgtcgccgt taaactcttt
     7261 tgctacgtcc agcgcgcctt tggcggtgtt gatatccgca cagcaaatta ccgtcaccca
     7321 gtcagcgttg gcttcgaagc acatacgcga aaggattttg cccgcatcag caattttggc
     7381 gtctgccagt acgattttat gcgggtagag cgctttcaga tcgcgaaccg cacgcacgcc
     7441 ttcgcccacg cacagaatgg tacccacttc gataatgtcg acttcttcgg caatcaggcg
     7501 agtggtttcg taggcgctat ccatagtctg gttgtccagc gcgacttgca acattggtaa
     7561 tgacatcttc aattccttct taagctgccg cgttggtgcg gtcaatgaga tccagtactt
     7621 cctgctcggt acggcaggcg cgtaaacggt cgaaattctc ttcatcttca aacaggttga
     7681 cgatctgcat gatgcccact tcctggtgag tgttggcatc gaccgccgcc atggtgatga
     7741 gtatatccac cgggtcatta tcttcgtggt tgaactccag cggctttttc agcgtcacca
     7801 gcgcaaaacc ggttttctta acaccctctt ccgggcgacc gtgcggcatt gccaggcctg
     7861 gtgcgagcac aaagtacgga ccgtgctgtt caaccgcatc cagaatcgcc tggtagtaac
     7921 gcggctcgac aacatccgcc gccaccagca ggtcaacgcc gatttttact gcgtcctgcc
     7981 atgtctcagc ttcagcctgc aggcggatgg atttattctc cgccagcgaa tcacgtaatt
     8041 tcatggcgcg tccttacttc acatcctgcg ggaaatgcgc tttgatcact tccagcagtt
     8101 tcgggccaaa gtcggcagga gagagcatgt tgcgcacgcc aaccacgtat ttgttgccgg
     8161 tcacggtgat ttcgcccgca atgtgcgtag aagcgatgat gatatccgcg ccgctcaact
     8221 cgcttttgta ttcgccaacc gcgcagctat ttaccgtgtg gtcaatgttt gattgggtta
     8281 aaaactggtc cactttcatc ttcatgatca tggaactgcc ttgtccgttg ccacacacag
     8341 ccagaatacg tacggtcata atcaaaactc cttattaagc agactgttct gccagttgtt
     8401 tttctgcatc ttcttctgcg cgcagtgcgc ggccagcgaa gaacatataa gccagtgcaa
     8461 ttacaatgat gacggccata aaggcgatac cgatggagaa gaagccctgc atcatcggcg
     8521 gtgccagaat cgaccagtcc gccatgccca tccaggcgct cataccggtg agtttcaccg
     8581 cccagacgca gccaaagatt tcaatcatcc ccatcaccag acatatcttc agcgccgcac
     8641 gccagccgcc gaagtggtta gcgaacacgc cgatggtggc gttggagaag aacatcggga
     8701 taaagccggg aataatcagg atcgaggaac cgcaggcgac caggatgcca accgcaatca
     8761 gctgaccgat ggtgccccac ataaagcccc aaaccacggc gttcggcgcg aagctataga
     8821 tagccgcaca gtcaatcgcc agaaccgcac cagggatcag gcgctgggag ataccgttaa
     8881 atgcttcaga gagttccgcc acaaacatgc gcacgccctg agtgatgatg aagatcgcca
     8941 ccgcgaagga gaaaccagtt tgcaggatgt acaccgtcca gttcactttg cctgccatcg
     9001 cctgcacggt gtcgataccg aaggagagca gaatggcacc aaagaagatg gtcatcacaa
     9061 tcgccgtgga gacgatgttg tcgtggaaaa tattcagcca gcctggcagt ttgaggtctt
     9121 caacgctttc ctcttttttg ccgaggaacg gcgcaacttt ataggcaatc catgatgcaa
     9181 actgttgctg gtgaccgatg gagaaaccgc agccgtccgt cacttcctga gtcggcttgt
     9241 acatcatgtt ggaggtaatg ccccagtaga gcgaaaccag aatcgccgtg cagataatgg
     9301 ttgtccacat ggagtagccg aaaataaaca acgtaacggc aatcagcccc gcctgctgga
     9361 acatgatatg accagtcagc atgatggtac gaataccggt aatgcgacgc agcagcacgt
     9421 aacagatgtt cagcgccagc gctaacaaca ccgcgtaacc tacccagcta taggcatcgc
     9481 ccatgcggtc gatggttgcc atcatcgacg cgtaagtatc ggaaattgcg ccgttaatgc
     9541 cgtagacttc ggacattttc gccaccaccg gtttgaaggt gctggtgagg atgccggacc
     9601 ctgcctgcaa caacatgaaa ccaattatgg ttttaatcgt gcctttaata ataacgctga
     9661 cacttttgcg cagtaggatg tagcccagac aggtcacaat acccagcaac aacggggcat
     9721 tggtcatgac ctggttaaaa aacacggtaa agatgttgta gaggatctcc ataacgatct
     9781 cctgaagaag aggtaaccgg gtattccaca cacccggaat gttgtgcact cactctaatt
     9841 ttcaaaagta atcacaacaa gattatttat gattaaatgt gacgcgaccc gcaaataatt
     9901 ccacatggat agaatgggat tcacacaacg aaacaactat tcatttaaat tcatttagtt
     9961 aattgaatta cacattccat taaatctttc cacactcaaa atcaacaacc acttccctgg
    10021 caaaaagtca aaaaatggtc attgccttcc tgaaaaaata gtgtcaggat taatcaaaac
    10081 caatcacaca ttgattcgaa ttgaacaatc atgaaggtaa atggcgatga gtaaagtgaa
    10141 aagtatcacc cgtgaatcct ggatcctgag cactttcccg gagtggggta gctggctgaa
    10201 tgaagaaatt gaacaagagc aggtcgcccc tggcacattt gcgatgtggt ggctaggctg
    10261 caccgggatc tggttgaaat cagaaggtgg caccaacgtt tgcgttgatt tctggtgcgg
    10321 cactggcaaa caaagtcacg gtaacccgtt aatgaaacag ggtcaccaga tgcagcgcat
    10381 ggctggcgtg aaaaaactgc agccaaacct acgcaccacc ccgtttgttc ttgatccgtt
    10441 tgcgattcgc cagatcgacg cggtactggc gactcacgat cacaacgatc atatcgacgt
    10501 taacgtcgct gctgccgtga tgcagaactg tgctgatgac gtaccattca ttggaccgaa
    10561 aacctgcgtt gatttgtgga ttggctgggg cgtaccgaaa gagcgttgca tcgtggtcaa
    10621 accgggcgat gttgtaaaag tgaaagacat tgaaattcat gcgcttgacg ctttcgaccg
    10681 tactgcactg atcaccctgc ctgccgatca aaaagcggct ggcgtactgc cggacggcat
    10741 ggacgatcgc gcggtgaact atctgttcaa aacgcctggc ggctccctgt atcacagcgg
    10801 cgactcccac tactctaact attatgcgaa gcacggtaac gaacatcaga tcgatgtggc
    10861 gttaggatcg tatggtgaga acccgcgcgg tattaccgac aaaatgacca gcgccgatat
    10921 gctgcgtatg ggtgaagccc tgaatgcgaa agtggttatc ccgttccacc acgacatctg
    10981 gtcaaacttc caggccgatc cgcaagagat ccgcgtgctg tgggagatga aaaaagatcg
    11041 tctgaagtat ggcttcaagc cgtttatctg gcaggttggc ggcaaattta cctggccgct
    11101 ggataaagac aacttcgagt accactatcc gcgcggtttc gatgattgct tcactattga
    11161 accggatctg ccgttcaagt cattcctgta atctgtcgtt tataccggat agcgtggaga
    11221 agttcgcgct atccggcgta gtatttcctg gctatttcaa atatcatcta aaaaaatcaa
    11281 attttatcgg aatagctcat gactgaagca caaagacatc aaatcctcct ggaaatgctc
    11341 gcacaattgg gctttgtgac cgttgagaaa gtcgttgagc gtctgggaat ttcgcctgcc
    11401 actgcgcgac gcgatatcaa taaacttgac gaaagcggca aactgaaaaa ggtgcgcaat
    11461 ggcgcagaag ctattaccca acagcgcccg cgctggacgc cgatgaatct gcatcaggcg
    11521 cagaatcacg atgaaaaagt acgtattgct aaagcggcct cgcaaatggt taatccgggc
    11581 gaaagcgtag tcatcaactg cggctccacc gcgtttctgc ttgggcggga aatgtgtggc
    11641 aagccagtgc aaatcatcac taattatcta ccgctggcaa attacctgat cgatcaagaa
    11701 catgacagcg tgatcattat gggcggacag tacaacaaaa gtcagtccat cactttaagc
    11761 ccgcagggca gcgaaaacag tctctatgcc gggcactgga tgtttaccag cggcaaagga
    11821 ctgaccgcag aagggttgta taaaaccgat atgctgacgg caatggcaga gcagaagatg
    11881 ctgagcgtgg tagggaaact ggtggtactg gttgatagca gtaagattgg cgaacgcgcg
    11941 ggaatgcttt ttagccgtgc cgatcaaatt gatatgctga tcaccggcaa aaatgctaac
    12001 ccggaaatcc tgcaacaact ggaagcgcaa ggggtcagca ttctgcgtgt ttaaagatgc
    12061 tggcggaaaa atgtcactgc agcatctaac gcctcaggcg taatgcggtg acgcacgcct
    12121 ggctgccatg aacaggttag cagtttatcc cgtcccgtct cgcttaaggc ctgctgcaaa
    12181 cgtagtgatt cgtcggcagg cacaacgtca tcgtcgaggc catgccacag cagtagaggt
    12241 ctgtcactaa gttgttccag gtggtttgtc gcttcccact ctgccagcgg cgcgacaatg
    12301 ttattgaatt cattctgctg tgctgtcgtt tcaggtatca gcggtggaaa cagtgaacgg
    12361 gcgagtgatg taaaatagcc cgatcccatc atgctggcgg tacatctcac cgtggggtgg
    12421 cgagcggtaa tccccagtgc cgtcatcgcg cccatcgacg caccaccgac tgccagacgg
    12481 tcatcaagca gccagttttc ttcggctatt gccgcacgta aagtagtgaa ttcctgcata
    12541 ctttgtagca agatttgcca gaattgattt aaccgccgcg ctgcgtcacc actaaaacgg
    12601 ctaccgtgat cgggcgcatc cggcatgatc actcgcaaac cagcctgcgc cagcgcaacg
    12661 gcaaaatagc tatacaccag actggatgaa gtaaagccgt gataaaaaat tacgcacggt
    12721 aacggggtat ctttttgccc gacaggataa gcatgaagaa cgggaatatc tgccagctcg
    12781 cgtgattcta tttcaatcat ctgtctctcc tttttgtctg gtatgacatg aagaaatcct
    12841 tagcacaagt ttctgctggc ggtattagtt cataatgttg agatgtgggt tacgctttcg
    12901 tcacgttttc attcgaaaat cgcgtaccag gtaacaattc gggaacattc ccccaaagtt
    12961 aaattaataa ggcactacac tatggttagc aggaaacgta atagcgttat ttaccggttt
    13021 gccagtttat tattggtgtt gatgttaagt gcctgtagcg cactgcaagg tacgccacag
    13081 ccagcaccgc cagtaacgga ccatccgcaa gagattcgcc gcgaccagac gcaagggtta
    13141 cagcgaatag gcagcgtaag cacgatggtt cggggttctc cggatgacgc attagcagaa
    13201 atcagagcga aagctgtcgc tgcaaaagct gattattacg ttgtcgtcat ggtggacgaa
    13261 accatcgtga caggacagtg gtattcacaa gccattttgt accgtaaata aatcagacaa
    13321 cttacatagt cagctttaca ttgctttgcg tccctatgcg tttacctgtg cacaataaat
    13381 taccggctgg aagcctgggc gtttattacc gcgatggaat gccctgcaac atgtggggaa
    13441 acgaaaaatg gagctgacga tgaaacaatt acttgcctca ccctcgctgc aattagtgac
    13501 ttatcctgcg agcgccacgg cacagtctgc agaattcgcc agtgctgact gcgtgacggg
    13561 cctgaatgaa ataggtcaga tctcagttag caatatttca ggggatccgc aggacgtgga
    13621 acgtatcgtg gcgttaaagg ccgatgaaca aggtgcgtca tggtaccgca ttattaccat
    13681 gtacgaagac cagcagcctg ataactggcg cgtacaggcg atactgtatg catgagtgtc
    13741 atctacagca aatgaacaat gccggatgcg gcgcgagcgc cttgtccggc ctacaatttt
    13801 cacatatttc atttagttaa tcgaaaccag cgtcgcatca gtcgtatacg cttacacaca
    13861 cactcccccc gtcgcccgca gcaataaatc attctggatc tgctctgaca accgtacgcc
    13921 gccgcgcgta tctaacatca cctgacacca cgcctgcgcc attggcggag aagcatattt
    13981 caacatttgc gcaccgcagc ccagcaggaa tagctgatga gtaatctctc gcctcagttc
    14041 ttcagctggc ttacgcagct gctgctgtaa acgacgaacc gcgcgatcaa aatagcgatc
    14101 ctgccctttc acttccacaa acgcttccga caataagtcg tatacgcccg cttgcttatt
    14161 gagaacgcgc agcacatcca ggcacataat attgccggaa ccttcccaaa tactgtttac
    14221 cggcatctcc cggtaaagcc gcggcagctc gctctcctcg caataaccaa tgccgcccag
    14281 cacctccatc gcttcggcca caaacggcat acctcttttg cagatcacaa attttgccgc
    14341 aggcgtaaat aaacgcgccc acagggcttc tttggcatcg gcacgccggt cccacgctcg
    14401 cgcaagacga aacagcaacg ccgtttgccc ttcaagctga agtgccatgc gacttaagac
    14461 atgacgcata aggggctgtt ggatcaatgg attaccaaaa acatggcgtt gatgtgcatg
    14521 ataaatcgcc agcgaaaatg cacggcgcat catggcatgg ctacccaggg cgcaatcaaa
    14581 acgcgtcatc ccacccattt tcaggatcag acgaattcct tccccttcct gccccaacaa
    14641 ccagccaatg gcatcctgaa actccacttc gcagctggcg ttagagcgat tacccagctt
    14701 atctttcagc cgctcgaggc gaatcgcgtt gcgttgcccg tcaggcaaaa agcgcggcac
    14761 aaaaaagcag gacaaaccgc ccgtagtctg cgccagcacc agatgcgcat cgctttgcgg
    14821 caccgagaaa aaccatttat gccccaccag ccgataagag ccatcttcca ggcgctctgc
    14881 gcgggtggtg ttgctcatga catcggaacc gccctgcttt tccgtcattc ccatgccaat
    14941 caacaaaccg cgtttttgcc cacctggcaa taagtgagaa tcgtagcgat cgctcagcag
    15001 cggcgtggtc cagtcctgaa acggcgcggg taacatctgt aacagcaatg gcgtggcggc
    15061 aaaggtcatg gttatcggac ataacgaccc tgcctcaacc tgtgcgtgca acataaaacg
    15121 cgccgcgcgc gccacaaatg cgccggagcg agcgtcttct tcccaggcaa gattgtgcac
    15181 ccgattggta catagcgcct gcatcagcag gtgccaggcg gggtgaaaac gcacatcgtc
    15241 cagacggcgt ccttgcgcat cgtagcgcaa taattcaggc ggattcacat tcgccagccg
    15301 ccccagttca agggattcag ccgttcctaa ctgctgacca atactggcta gaaaatcgct
    15361 atcccagccg gcaccttcac gcgttaccgc ttcgcagagc gcgccatcag acaggtataa
    15421 gttgctgtta tttaatggta taggttgatt aaaaacggtg tgagtttgcc agtgcactgt
    15481 gtctccctcc atcaatggca gtcaccatta gtatggtcac tgccatggat tcctgacaga
    15541 aagagattgc taaaacattc ttagccagca atgtaatgcg gaacgaaacg agaggtgtct
    15601 ttggtgatca gcgtgttatc ttcacggatc cccattccgc acgcttcatc atcgacaatc
    15661 cagctaccga tgagtgtgta gctatcgcca aatcgcggca gaggttgaaa cgcctggtag
    15721 atcatcggtt catcggcgta atcaccatca gcgtggtcaa cgacgttatt ctgaccgtcg
    15781 aaaatggtga cgtttccgcc ttcgcgcgag tagatcggtt tacgcacata gctttcgcca
    15841 gcggcaatct gcgatttttc accctcaaac caggatgcaa gaaggttagg atgaccaggg
    15901 aagaagcgcc aaagcagcgg cattagccct ttattactca agatactttt ccataacggc
    15961 tccacccatt gctcgcgacg cttgcacagc agcggaccgt tatcgtcacg catcatccac
    16021 tccagcggat acagcttaaa tgcacgctgg atgacattat catcaagatc ggtcagtaca
    16081 ccgccgacgc ccaaaccgag atcttcaatg tagataaacc gcgactcctg ccctgcctgc
    16141 tgagcgcagt cctgcaagta cagcacggta gtcctgtctt catcggtgtc ctgacagcag
    16201 cagaaataaa acggctcccg gctataaagc tcgctaaagc gtgaaatcag gcgttcctga
    16261 atggcattgt actgatcggc atcacgcgga ataatgccgc tgcgccgggc atcctccagc
    16321 cacaaccact ggaaataagc cgactcatac aacgaggtcg gcgtatcggc gttgtactcc
    16381 agcagcttca ccggcgcatt gccacaccag gcaaaatcca tccggccata cagcgaagga
    16441 tcgcgtgcgc gccagctttc ggcgatcaca tcccagtaca acggcgggat cgccagttgc
    16501 gtcaggatct cttcgtcttt aaccgcgcga tccaccacct caaggcacat ctgatgcagt
    16561 tccgcagtcg gtttttcgat ctgctcttca atctggcgca gagtaaaacg gtaagcccca
    16621 ctctcgtccc aatagatttc attgtcgatg atatgaaagt cgaaaccgtt gttagcggcg
    16681 atctggtcca gatcccgtcg cacaggaacg ttgtgtctca gcatgattag cctccccaat
    16741 gcccacgggc gctggaagaa cgaccatagc cgccgcgcga aacggttgac gcttttttgg
    16801 tggtaaagcc gcctgaagag taagactctt ttttgccaga accggagcgc caggagtaat
    16861 caccggaagt gttgcgccag acggggcggg aagcaaatga ggatccgccg ctgttataga
    16921 cgaacggctc atcgcgatct ttacgaataa ctcggcttaa caaaaatcca gaaacgaccg
    16981 ggatccaact ctgttcaaca ttgtcgtagt agcaattttc gtacttagac tgacaattct
    17041 gctgagtcat attcttcgga acatcggcat aaaatgccgc tttggcgttg ttccagccac
    17101 gcgcgcaaat gtcggcatta ttaccgtcat caatacaatc ctgcactgtc gaataaaacg
    17161 ttccgtcgcc gtcgttatcg acatcgccac tttcactcca tcctttgact attaaaaaaa
    17221 cggcaccgcc cattagcacc aacgttaagt atttttgtgc gtagcgattg cggcgcggct
    17281 caaaaggatt atgcggtctt ccaatgcgac tgatcgctcc gtgaccaaat ttactgttat
    17341 ttcttgattt gcgttttctg gccatgatcg tccattacca tgtcatacaa gctgcgttaa
    17401 aaataccacc agccagtgcg gcggttccca taaacattcc tgctgcggta ttgtgattaa
    17461 taattttttc gcttaatgcg ggcatataaa gtctgacacc agcaaaaacg agtaactgaa
    17521 tcaccagtgc aatcccgccc caggcgaaat agtctggaat actcaccgca ttgattgccg
    17581 cactggataa ggggatcacg taacccaaca atgtaccgct gaatgccagt gacgccgcgg
    17641 tattattgtt tttgattaat tgccattcgt tgtgcggtgt aattttggag tagataaaca
    17701 gaaaaataat caccatagcc acgccaataa aaaaataggc actaaaggca agaagtgaat
    17761 ccagtatatg cattgtaaac gtccttgtaa ttaaccaata atatgaagtg atgtcagcgg
    17821 gatatcgaca cccaacgcgc gcgaaaataa ccactctggc tcgccgagat cgttaaaaga
    17881 ttcctcacca tttaacagca aatattcgta agtatcttcg gttacttggc gttggtagcc
    17941 catggtaaaa ttatggacct cccatttggc atggttttga ttttctactt tttccagcat
    18001 gtagacgggt tcgatatttc ccggttcttc gctgttaaaa aatcgctgcc agcgtttttc
    18061 ctgccagttt aagatcattg cccccatcac tttggcgttg atcgcctcgc gccagtgact
    18121 ctctttactg ataccgtagc tctcttcata gacaaagagc ttgatatcat caatgtcatc
    18181 aatatcttcg ccgccggtgg tattgatttg cagaaattca tcgcctgaag tgtagtagcg
    18241 gaaaatctga ctaccgccgc ccagatcgat gtggctgaca gcggctaccg taaattcctc
    18301 acccggcagc gcaatcagca attcatcttc cagcaaacga aacgctaacg tgtcgagcgt
    18361 aaagccacta ttgagatgaa gtcccagcgg accgcgagcg atagcaggct tattatcctt
    18421 gccaaacaga cgctggaaaa aaccagacat attttatcct taaaatagtt gccagccttt
    18481 tagcgaggct ggcaaaaaaa ttactcgccc tgttggcgtt gcagtcttgc taatacatcc
    18541 tgggcgctac ttttattgct accgccaatt ccggcttccg ccagcttttc atcaagatcg
    18601 cgaccgtctg cgactttttc caactgtgca gcagcatcca ggcgagcctg acgttcggcc
    18661 tgacgcgttt gcaggcgttt taaggattct gccgctgtcg aaacgctgga agatgcgcca
    18721 acggtagagg ttgttacagc ctgttgtgca cgctgcatgg cttcagtggc tttaacgact
    18781 tccatttgct gctcaaactg agcgatacgc tgcgctgtcg ctgtaactgc tttttccacg
    18841 ccatcacggg agacttccag attcgacaac acttgctctt cagcggtaat gagattctca
    18901 aggcgcgcga tttcttcagc aacttcgtta atcaacgacg gattaacgtt cttgcttaac
    18961 gcttccagcg cacgggcttc cagactggct ttgcgctcac gtaaatcttt cagcttatcg
    19021 tgacttaatt tcacccgcgc cagcagatca acgcgagatt ttccggcttt atcgagttca
    19081 gctttagcgt cacgaatatg ctgttccagc atgcgcacgc cctaggtttc ttcaatggat
    19141 tcttccgcct gggagataaa cgatttcccc agcgtaaata aactttttaa aattcccata
    19201 aatacccgtc cttgtaatta gtgtgaatat tcttctgtaa tttcagccag atccaatgcg
    19261 ttatctacca gcgaggttat ctcaagcagg atatcttcaa gagaagattt aagcgataac
    19321 gccccgaaaa caatgtaata ctcttcctgt tgcacgctgg agattcctac cgatgacaac
    19381 ggcatcatct tctgatttct taataagaag gtatt
//
LOCUS       DAZFRN010000089        19242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFRN010000089 DAZFRN010000000
VERSION     DAZFRN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.089"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..463)
                     /locus_tag="ACHKUB_003056"
     CDS             complement(<1..463)
                     /locus_tag="ACHKUB_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO6656036.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVLRLDEGHR"
     gene            complement(623..835)
                     /gene="rpmE"
                     /locus_tag="ACHKUB_003057"
     CDS             complement(623..835)
                     /gene="rpmE"
                     /locus_tag="ACHKUB_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO6656037.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1038..3236
                     /gene="priA"
                     /locus_tag="ACHKUB_003058"
     CDS             1038..3236
                     /gene="priA"
                     /locus_tag="ACHKUB_003058"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO6656038.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3392..4417
                     /gene="cytR"
                     /locus_tag="ACHKUB_003059"
     CDS             3392..4417
                     /gene="cytR"
                     /locus_tag="ACHKUB_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO6656039.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4509..5468
                     /gene="ftsN"
                     /locus_tag="ACHKUB_003060"
     CDS             4509..5468
                     /gene="ftsN"
                     /locus_tag="ACHKUB_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO6656040.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5561..6091
                     /gene="hslV"
                     /locus_tag="ACHKUB_003061"
     CDS             5561..6091
                     /gene="hslV"
                     /locus_tag="ACHKUB_003061"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO6656041.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6101..7432
                     /gene="hslU"
                     /locus_tag="ACHKUB_003062"
     CDS             6101..7432
                     /gene="hslU"
                     /locus_tag="ACHKUB_003062"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO6656042.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7499..8425
                     /gene="menA"
                     /locus_tag="ACHKUB_003063"
     CDS             7499..8425
                     /gene="menA"
                     /locus_tag="ACHKUB_003063"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO6656043.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8518..9003
                     /gene="rraA"
                     /locus_tag="ACHKUB_003064"
     CDS             8518..9003
                     /gene="rraA"
                     /locus_tag="ACHKUB_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO6656044.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9088..9333)
                     /gene="zapB"
                     /locus_tag="ACHKUB_003065"
     CDS             complement(9088..9333)
                     /gene="zapB"
                     /locus_tag="ACHKUB_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO6656045.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9758..10603
                     /gene="glpF"
                     /locus_tag="ACHKUB_003066"
     CDS             9758..10603
                     /gene="glpF"
                     /locus_tag="ACHKUB_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO6656046.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10626..12134
                     /gene="glpK"
                     /locus_tag="ACHKUB_003067"
     CDS             10626..12134
                     /gene="glpK"
                     /locus_tag="ACHKUB_003067"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO6656047.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12270..13280
                     /gene="glpX"
                     /locus_tag="ACHKUB_003068"
     CDS             12270..13280
                     /gene="glpX"
                     /locus_tag="ACHKUB_003068"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6656048.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13377..14123
                     /gene="fpr"
                     /locus_tag="ACHKUB_003069"
     CDS             13377..14123
                     /gene="fpr"
                     /locus_tag="ACHKUB_003069"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO6656049.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14128..14556)
                     /gene="uspD"
                     /locus_tag="ACHKUB_003070"
     CDS             complement(14128..14556)
                     /gene="uspD"
                     /locus_tag="ACHKUB_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO6656050.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14583..14882)
                     /locus_tag="ACHKUB_003071"
     CDS             complement(14583..14882)
                     /locus_tag="ACHKUB_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO6656051.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15094..15534)
                     /locus_tag="ACHKUB_003072"
     CDS             complement(15094..15534)
                     /locus_tag="ACHKUB_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6656052.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15635..16234
                     /locus_tag="ACHKUB_003073"
     CDS             15635..16234
                     /locus_tag="ACHKUB_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO6656053.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16342..17109
                     /gene="tpiA"
                     /locus_tag="ACHKUB_003074"
     CDS             16342..17109
                     /gene="tpiA"
                     /locus_tag="ACHKUB_003074"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO6656054.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17164..17919)
                     /gene="cdh"
                     /locus_tag="ACHKUB_003075"
     CDS             complement(17164..17919)
                     /gene="cdh"
                     /locus_tag="ACHKUB_003075"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO6656055.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(18026..19015)
                     /gene="sbp"
                     /locus_tag="ACHKUB_003076"
     CDS             complement(18026..19015)
                     /gene="sbp"
                     /locus_tag="ACHKUB_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO6656056.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEDFGGWTKAQKEHFANGGTFDQISKR"
BASE COUNT         4700 a         4978 c         5047 g         4517 t
ORIGIN      
        1 gccggtgacc ttcatccagt ctcaggacgc cgccgcgcac cagccacagt gactcgctgc
       61 ggctgtaacc gtcctcaccg ggaaacaggt gctcaaaata caggcttctg ccgccgttat
      121 cactgagtat cagtgtgtta tcgcgcagct gtaagcggat atccgcaggc attttccagc
      181 cggggccgag gctccccacc ggcgcgggcg tttttgtccg gtaactgctg taggtgcggg
      241 agaggatgaa cggcagcggg ccgggcaggg cgatgtcggt ttcaccggga aggacctttg
      301 caccgagcag gggattgacc ggatggccgg acgtcactcc gccggggcac accgaacagg
      361 ccacgccggt gggggcacca atgcgcaccc cggctgaacc ctgaacaatg ctaccgccat
      421 actgcgtcat gtcgccctga cgcgctgccg gttttccgct catcgtaaaa tccttttgaa
      481 ataaataaga accagaagcc tagcgccagt caacaacagg aaatgataat gagttaacac
      541 cccggaattt ttcagaattt ataaaaggca aattacaggc acaaaaaaag cgccgtgcgg
      601 cgcttttttc ggaaatccgg tcttatttgc tgcccgggat gttgaaacgc ttgttgaagc
      661 ggtcaacacg gccaccggta gcaacatcac gctgtttgcc agtgaagaac gggtggcact
      721 tgctgcacac gtcgaggttc aggtcatgac caacggtgga gcggattttc attacgttac
      781 cgcaagagca gctagcagta atttcttcgt atttcgggtg aatatctttt ttcatgggaa
      841 aacctcggtt taaggccgcg tcgctcttcc agccctaacg ccagacacca cgcgatgtta
      901 aaagtatagc ttcaatacga tcatttcgta cgaaggcgcg aaatcataca gaaattaacc
      961 agcgtatgca aactgatccg cactcttcta cggcaatgtg tatactaacc caccgaattt
     1021 caagtcagga tgatgctatg cccgttgccc acgttgcctt gcccgttccg cttcctcgta
     1081 cctttgacta tctgctgcca gaaggcatga cggttaaagc cgggtgtcgc gtgcgcgtgc
     1141 cgtttggcaa acagcaggag cgcatcgggg ttgtggtgtc agttagcgat gtcagtgagc
     1201 tgccgcttaa cgagctgaaa gcggtagtgg aagtgctgga cgttgagccg gtatttactc
     1261 actccgtctg gcggttactg ctctgggcgg cagattacta tcatcatccg attggcgatg
     1321 tgctgtttca tgccttgccg attttactgc gccaggggcg gcctgcggcg aacgcgccga
     1381 tgtggtactg gtttgccact gaacaaggcc aggcggtgga tctgaacagc ctgaaacgct
     1441 cccccaagca acaacaggcg ctggcggcgt tacggcaagg caaaatctgg cgcgaccagg
     1501 tcgccacgct cgaatttaat gatgccgcgt tgcaggcgct acgcaaaaaa ggtctgtgtg
     1561 atttagcaag tgaaacacca gagtttagcg actggcgaac gaactatgcc gtttctggtg
     1621 agcggttgcg attgaatacc gaacaggcca ccgccgttgg cgcaattcat agcgcggcag
     1681 atactttttc tgcctggctg ctggcgggcg ttaccggttc cggtaaaacg gaggtttatc
     1741 tcagtgtact ggaaaacgtg ctcgctcagg gcaaacaggc gctggtgatg gtgccggaaa
     1801 tcggcctgac accgcaaact atcgcccgtt ttcgtgaacg ttttaatgcc cccgtggaag
     1861 ttctgcattc cggcctgaac gacagcgagc ggctttcggc gtggttgaaa gcgaaaaatg
     1921 gcgaggcggc gattgtgatc ggcacccgct ccgcgctgtt tacgccgttt aaaaatctcg
     1981 gcgtgattgt cattgatgaa gagcacgaca gctcctacaa gcagcaggaa ggctggcgct
     2041 atcatgcccg cgacctggcg gtgtatcgtg cgcatagcga gcaaatcccg attattcttg
     2101 gctccgcaac gcccgcgctg gaaacgttgt gcaacgtcca gcagaaaaaa taccgcctgc
     2161 tgcgcctgac gcgccgggcg gggaatgcgc gtccggcaat tcaacacgtg ctggatttaa
     2221 aaggtcagaa ggtgcaggca ggtctggctc cggcgttaat cacccgtatg cgccagcatt
     2281 tacaggccaa caatcaggtt atcctctttc ttaatcgtcg gggttttgcg cctgcgctac
     2341 tgtgccacga ctgcggctgg attgccgaat gcccacgttg cgatcactac tacacgctgc
     2401 atcaggcgca acagcatttg cgctgtcacc actgtgacag tcagcgtccg gtgccgcgcc
     2461 agtgcccttc ctgcggttcc acgcacctgg tacccgtggg gctgggcacc gaacagcttg
     2521 aacagacgct tgcgccgctg ttccctgatg tgcccatttc tcgtatcgac cgcgatacca
     2581 ccagccgcaa aggggcgctg gaacagcaac tggcggaagt acatcgcggc ggtgcgagga
     2641 ttttgatcgg cacacaaatg ctggcgaaag gtcaccactt tccggatgtg acgctggtgg
     2701 cgttactgga tgtggacggc gcgctgtttt ctgccgattt tcgctcggca gaacgtttcg
     2761 cccagcttta cacccaggtc gccggtcgtg ccgggcgtgc gggtaaacag ggcgaagtgg
     2821 tgctgcaaac gcaccatccg gaacatcctc tgttgcaaac gttgctctat aaaggctacg
     2881 acgcctttgc cgaacaggcg ctggctgagc gacgcatgat gcagctgccg ccgtggacca
     2941 gccatgtgat tgtgcgtgcg gaagatcata acaatcagca tgcgccgctg ttcctgcaac
     3001 aactgcgtaa tctgatcctt tccagcccgc tggcagacga caaactgtgg gttctcggcc
     3061 cggttccggc tctggcacct aagcgtggtg gccgctggcg ctggcagatc ttgttgcagc
     3121 acccttcccg cgtgcgcttg caacacatca ttagtggtac gctggcgctt attaatacaa
     3181 taccggattc ccgcaaggtg aaatgggtgt tggatgtcga tcccatcgaa ggctaatgcg
     3241 caaacgttgc gaggcggatc gaaaaattca atattcatca cacttttcat gaaaattctg
     3301 taaccgtttt cacgcgctat ctgctaaaaa tgttgccgat gtgaagtaaa catggatgta
     3361 gtacgcctga cgtgccaggc gaggagtgag tgtgaaagcg aagaagcagg aaactgccgc
     3421 gaccatgaaa gacgttgccc tcaaggcaaa agtctctaca gcgactgtct cccgagcatt
     3481 aatgaatccc gataaagtct cccaggccac ccgtaatcgg gttgaaaaag cggcccggga
     3541 agtgggttat ttaccgcagc ctatggggcg caacgtcaag cgtaatgaat cccgcaccat
     3601 tctggtgatt gtcccggata tctgcgatcc cttctttagc gaaattattc gcggtatcga
     3661 agttacggcg gcaaatcacg gatatctggt gctgattggc gactgtgcgc atcaaaatca
     3721 gcaggaaaaa acctttatcg atttgatcat caccaagcaa attgatggca tgttgctgct
     3781 gggttcaagg ctgccgtttg atgccagcat tgaggaacag cgtaatctgc cgccgatggt
     3841 gatggcgaac gaattcgcac cggaactgga gctacctaca gttcatatcg acaatctgac
     3901 cgccgcattt gatgcagtaa attatctata tgagcaaggg cataaacgga ttggctgtat
     3961 agccggtccc gaagagatgc cgctgtgtca ctaccgcctg caaggttacg ttcaggcgct
     4021 gcgtcgctgc ggcattatgg ttgatccgca atacatcgcc cgtggcgact tcaccttcga
     4081 agccggaagc aaagcgatgc agcagctgct tgatcttcca caaccgccta ctgctgtctt
     4141 ctgccatagc gatgtgatgg cgctcggcgc actttctcag gcaaaacgcc aggggctgaa
     4201 agtcccggaa gacctttcca taatcggttt tgataacatc gacctgacgc aattttgtga
     4261 tccgccgctg acaaccatcg cgcagccgcg ttacgaaatc ggtcgggaag ctatgctgtt
     4321 attgcttgat caaatgcagg ggcaacacgt tggcagtggc tctcgtttaa tggactgcga
     4381 acttatcatc cggggatcaa cacgcgcgtt accttaaagt aaacctttaa gactgccgtg
     4441 actggtcaaa ggcccgccgc ttaagtaaca tggcgggctg acgaacgaat aaatacagcg
     4501 aaacgatagt ggcacaacga gattatgtac gccgcagcca accggcacct tcgcggcgaa
     4561 aaaagagcac ctcacggaaa aagcaacgaa atctgcctgc ggtttctccc gctatggtcg
     4621 ctattgctgc cgccgttctt gtgaccttta tcggtggtct gtacttcatt acgcatcaca
     4681 agaaagaaga gtccgagacg ctgcaaagcc agaaagtgac cggtaacgga ctaccaccaa
     4741 aaccagaaga acgctggcgc tacattaaag agctggaaag tcgccagccg ggtgtgcgtg
     4801 cgcccacaga accttctgcc ggtggtgaag tgaaaacgcc ggagcaactg acaccagaac
     4861 aacgtcagct tcttgaacaa atgcaggctg atatgcgcca gcagccaacg cagctggttg
     4921 aagttccgtg gaatgaacag acgccggaac agcgtcagca aacgctacaa cgccagcgtc
     4981 aggcgcagca attagcggag cagcaacgcc tggctcagca gtcgcgtacg actgaacaaa
     5041 gctggcagca gcagacgcgt acgtcgcaag ccgcgccggt acaggcgcaa ccgcgccagt
     5101 ctaaaccggc ttctactcag cagccgtacc aggatctgct gcaaacccct gcgcacacga
     5161 ccgcgcagtc gaagccgcag caagctgcgc cggttgctcg tgccgctgac gcgccaaaac
     5221 cgacggcgga gaaaaaagac gaacgccgct ggatggtgca gtgcggttcg ttcagaggcg
     5281 cggaacaggc agagacggta cgtgctcagc tggcgttcga aggatttgac tcgaaaatca
     5341 ccaccaacaa tggctggaat cgtgtggtca ttggcccggt gaaaggcaaa gagaacgcag
     5401 acagcaccct caatcggttg aagatggcgg gtcatacaaa ctgcattcgg ctcgccgccg
     5461 ggggttgaaa ccctcaaaat cccccccatc tataattgca ttatgccccg tacttttgta
     5521 cggggtttgt actctgtatt cgtaaccaag gggtcagctc gtgacaacta tagtaagcgt
     5581 acgccgtaac ggccatgtgg tcatcgctgg tgatggtcag gccacgttgg gcaataccgt
     5641 aatgaaaggc aacgtgaaaa aggtccgccg tctgtacaac gacaaagtca tcgcgggctt
     5701 tgcgggcggt actgcggatg cttttacgct gttcgaactg tttgaacgta aactggaaat
     5761 gcatcagggc catctggtca aagccgccgt tgagctggcg aaagactggc gtaccgatcg
     5821 catgctgcgc aaacttgaag cactgctggc agtcgcggat gaaacagcat cgcttatcat
     5881 caccggtaac ggtgacgtgg tgcagccaga aaacgatctt attgctatcg gctccggcgg
     5941 cccttacgcc caggctgcgg cgcgtgcact gttagaaaat actgaactga gtgctcgtga
     6001 aattgctgaa aaggcgttgg atattgcagg cgacatttgc atctatacca accatttcca
     6061 caccatcgaa gaattaagct acaaagcgta aggatctccc atgtctgaaa tgaccccacg
     6121 cgaaatcgtc agcgaactgg ataagcacat catcggccag gacaacgcca agcgttctgt
     6181 ggcgattgct ctgcgtaacc gctggcgtcg catgcagctc aacgaagagc tgcgccatga
     6241 agtgaccccg aaaaatatcc tgatgatcgg cccgaccggt gtcggtaaaa ctgaaatcgc
     6301 ccgtcgtctg gctaagctgg cgaatgcgcc gttcatcaaa gttgaagcga ccaaattcac
     6361 cgaagtgggc tacgtcggta aggaagtgga ttctattatt cgcgatctga ccgatgccgc
     6421 cgtgaaaatg gtacgcgtcc aggctatcga gaaaaaccgt tatcgcgctg aagaactggc
     6481 agaagaacgt attctcgacg tgctgatccc acctgctaaa aacaactggg gacagaccga
     6541 acagcagcag gaaccgtccg ctgctcgtca ggcattccgc aaaaaactgc gtgaaggcca
     6601 gcttgatgac aaagaaatcg agatcgatct tgccgcagca ccgatgggcg ttgaaattat
     6661 ggctcctcca ggcatggaag agatgaccag ccagctgcag tccatgttcc agaacctggg
     6721 cggccagaag caaaaagcgc gtaagctgaa aatcaaagac gccatgaagc tgctgattga
     6781 agaagaagcg gcgaaactgg tgaacccgga agagctgaag caagacgcta tcgacgctgt
     6841 tgagcagcac gggatcgtgt ttatcgacga aatcgacaaa atctgtaagc gcggcgagtc
     6901 ttccggtccg gatgtttctc gtgaaggcgt tcagcgtgac ctgctgccgc tggtagaagg
     6961 ttgcaccgtt tccaccaaac acgggatggt caaaactgac cacattctgt ttatcgcttc
     7021 tggcgcgttc cagattgcga aaccgtctga cctgatcccg gaactgcaag gtcgtctgcc
     7081 aatccgcgtt gaactgcagg cgctgaccac cagcgacttc gagcgtattc tgaccgagcc
     7141 gaatgcctct atcaccgtgc agtacaaagc actgatggcg actgaaggcg taaatatcga
     7201 gtttaccgac tccggtatta agcgcatcgc ggaagcggca tggcaggtga acgaatctac
     7261 cgaaaacatc ggtgctcgtc gtttacacac tgttctggag cgtttaatgg aagagatttc
     7321 ctacgacgcc agcgatttaa gcggtcaaac tatcatcatt gacgcagatt atgtgagcaa
     7381 acatctggat gcgttggtgg cagatgaaga tctgagccgt tttatcctat aatcgcgttc
     7441 aatcattttc atcattgttt gatggggctg aaaggcccca tttttattgg cgcgtattat
     7501 gactgaacaa caaattagcc gaactcaggc gtggctggaa agtttacgac ctaaaaccct
     7561 ccccctcgcc tttgctgcaa ttatcgtcgg gacggcgctg gcatggtggc aaggtcactt
     7621 cgatccgctg gtcgccctgc tggcactaat taccgccggg ctattacaga tcctttctaa
     7681 cctcgccaat gattacggcg atgcggtaaa aggcagcgat aaacctgacc gcattgggcc
     7741 gctacgcggc atgcaaaaag gggtcattac ccagcaagag atgaaacggg cgctcattat
     7801 taccgtcgtg ctcatctgtc tctccgggct ggcactggtt gcagtggcat gccatacgct
     7861 ggccgatttt gtcggtttcc tgattcttgg cgggttgtcg atcattgccg ctatcaccta
     7921 caccgtgggc aatcgtcctt atggttatat cggtctgggt gatatttccg tactggtttt
     7981 ctttggctgg ttgagtgtca tggggagctg gtatttacag gctcatacat tgattccggc
     8041 actgatcctt ccggcgaccg catgcggcct gctggcaacg gcagtactga atattaataa
     8101 cctgcgtgat atcaatagcg accgcgaaaa tggcaaaaac acgctggtgg tgcgcttagg
     8161 tgaagtgaac gcgcgtcgtt atcatgcctg cctgctgatg ggctcgctgg tgtgtctggc
     8221 gctgtttaat ctcttttcgc tgcatagcct gtggggctgg ctgttcctgc tggcggcacc
     8281 attactggtg aagcaagccc gttatgtgat gcgggaaatg gacccggtgg cgatgcgacc
     8341 aatgctggaa cgtactgtca agggagcgtt actgactaac ctgctgtttg ttttagggat
     8401 attcctaagc cagtgggcag cataactgac aaatatcaat taacaattga tgattttgcc
     8461 aacagcctac atagcgcgat atactgaaaa ttctcgcagc aactgaatgt taagcctatg
     8521 aaatacgata cttccgagct ttgtgacatc tatcaagaag atgttaacgt cgtggaaccg
     8581 ctgttctcca actttggcgg acgggcgtcg tttggcggac aaataatcac ggtaaaatgt
     8641 ttcgaggaca acgggttgct gtacgatctg ctcgaacaga atggccgtgg tcgtgttctt
     8701 gtcgtcgatg gcggtggttc tgttcgtcgc gcactggtcg atgctgaact ggcgcgtctg
     8761 gcagtacaaa atgaatggga aggtctggtc atttacggcg cggtgcgtca ggtagatgac
     8821 ctggaagagt tggatatcgg catccaggcg atggcggcaa ttccggttgg tgccgctggc
     8881 gaaggcattg gcgaaagcga tgtccgcgtc aattttggcg gtgtcacctt cttctccggc
     8941 gaccatcttt atgccgacaa taccgggatt attctttctg aagatccgct ggatattgaa
     9001 tgatagaaaa ggcaccgcaa ggtgcctttt ttctgcgtta cctgttggcc tacacagtaa
     9061 agaaattacg cggaagatga agcgtaatca gacctcttcc atgcgaccca gcagggcctg
     9121 cagacgttcc tgccagccgt tctgctgttc tttcagatgg ttgttctcac gctccagctc
     9181 ttcgcgctga tgctgggcat tttgaacttc ctgcgacagt gagttgtttt tttctttcag
     9241 ctcttcgatt tccatctgca acagagtgat ggtatcaatc gcctgctgta cttttgcttc
     9301 cagtttctca aacacttcta atgacattgt catacctctc ctgaattgca aggcgttgat
     9361 ggataaaaat cctcgtcccg attaccggtg acgccttaat aaatacgagc gcactttagt
     9421 tagctccgat tgtatgaagc cgcgccatcg ctgtccagcg gcacgccttg cagattacgg
     9481 tttgccacac ttttcatcct tctcctggtg acataatcca catcaatcga aaatgttaat
     9541 aaatttgttg cgcgaatgat ctaacaaaca tgcatcatgt acaatcagat ggaataaatg
     9601 gcgcgataac gctcatttta tgacgaagca cacacatttt aagttcgata tttctcgttt
     9661 ttgctcgtta acgataagtt tacagcatgc ctacaagcat cgtggaggtc cgtgactttc
     9721 acgcatacaa caaacattaa ctcttcagga tccgattatg agtcaaacat caaccttgaa
     9781 aggccagtgc attgctgaat tcctcggtac cgggttgttg attttcttcg gtgtgggttg
     9841 cgttgcagca ctaaaagtcg ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg
     9901 gggactgggg gtggcaatgg ccatctacct gaccgcaggg gtttccggcg cgcatcttaa
     9961 tcccgctgtt accattgcat tgtggctgtt tgcctgtttc gacaagcgca aagttattcc
    10021 ttttatcgtt tcacaagttg ccggcgcttt ctgtgctgcg gctttagttt acgggcttta
    10081 ctacaattta tttttcgact tcgagcagac tcatcacatt gttcgcggca gcgttgaaag
    10141 tgttgatctg gctggcactt tctctactta ccctaatcct catatcaatt ttgtgcaggc
    10201 tttcgcagtt gagatggtga ttaccgctat tctgatgggg ctgatcctgg cgttaacgga
    10261 cgatggcaac ggtgtaccac gcggcccttt ggctcccttg ctgattggtc tactgattgc
    10321 ggtcattggc gcatctatgg gcccattgac aggttttgcc atgaacccag cgcgtgactt
    10381 cggtccgaaa gtctttgcct ggctggcggg ctggggcaat gtcgccttta ccggcggcag
    10441 agacattcct tacttcctgg tgccgctttt cggccctatc gttggcgcga ttgtaggtgc
    10501 atttgcctac cgcaaactga ttggtcgcca tttgccttgc gatatctgtg ttgtggaaga
    10561 aaaggaaacc acaactcctt cagaacaaaa agcttcgctg taatatgact acgggacaat
    10621 taaacatgac tgaaaaaaaa tatatcgttg cgctcgacca gggcaccacc agctcccgcg
    10681 cggtcgtaat ggatcacgat gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa
    10741 tctacccaaa accaggttgg gtagaacacg acccaatgga aatctgggcc acccaaagct
    10801 ccacgctggt agaagtgctg gcgaaagccg atatcagttc cgatcaaatt gcagctatcg
    10861 gtattacgaa ccagcgtgaa accactattg tctgggaaaa agaaaccggc aagcctatct
    10921 ataacgccat tgtctggcag tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg
    10981 acggtttaga agattatatc cgcagcaata ccggtctggt gattgacccg tacttttctg
    11041 gcaccaaagt gaagtggatc ctcgaccatg tggaaggctc tcgcgagcgt gcacgtcgtg
    11101 gtgaattgct gtttggtacg gttgatacgt ggcttatctg gaaaatgact cagggccgtg
    11161 tccatgtgac cgattacacc aacgcctctc gtaccatgtt gttcaacatc cataccctgg
    11221 actgggacga caaaatgctg gaagtgctgg atattccgcg cgagatgctg ccagaagtgc
    11281 gtcgttcttc cgaagtatac ggtcagacta acattggcgg caaaggcggc acgcgtattc
    11341 caatctccgg gatcgccggt gaccagcagg ccgcgctgtt tggtcagttg tgcgtgaaag
    11401 aagggatggc gaagaacacc tatggcactg gctgctttat gctgatgaac actggcgaga
    11461 aagcggtgaa atcagaaaac ggcctgctga ccaccatcgc ctgcggcccg actggcgaag
    11521 tgaactatgc gttggaaggt gcggtgttta tggcaggcgc atccattcag tggctgcgcg
    11581 atgagatgaa gttgattaac gacgcctacg attccgaata tttcgccacc aaagtgcaaa
    11641 acaccaatgg tgtgtatgtg gttccggcat ttaccgggct gggtgcgccg tactgggacc
    11701 cgtatgcgcg cggggcgatt ttcggtctga ctcgtggggt gaacgctaac cacattatac
    11761 gcgcgacgct ggagtctatt gcttatcaga cgcgtgacgt gctggaagcg atgcaggccg
    11821 actctggtat ccgtctgcac gccctgcgcg tggatggtgg cgcagtagca aacaatttcc
    11881 tgatgcagtt ccagtccgat attctcggca cccgcgttga gcgcccggaa gttcgcgaag
    11941 tgactgcatt gggtgcggcc tatcttgctg gtctggcggt cggtttctgg cagaacctcg
    12001 acgagctgca agagaaagcg gtgattgaac gcgagttccg tccaggcatc gaaaccactg
    12061 agcgtaatta ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg gcgtgggaag
    12121 aacacgacga gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcctgaa
    12181 accgtgcggg ggcaaccccg cacacatcaa taatcccacc cttcccctgt gctacacttc
    12241 gcgccattcc ttactgctta gagtttgcta tgagacgaga acttgccatc gaattttccc
    12301 gcgtcaccga atctgcggcg ctggctggct acaaatggtt aggacgcggc gataaaaaca
    12361 ccgcggacgg cgcggcggta aacgccatgc gtattatgct caaccaggtc aacattgacg
    12421 gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc gatgctctac attggtgaaa
    12481 aagtcggtac tggtcgcggc gacgcggtag atattgctgt tgatccgatt gaaggcacgc
    12541 gcatgacggc gatgggccag gctaacgcgc tggcggtgct ggcagtaggc gataaaggct
    12601 gcttcctcaa tgcgccggat atgtatatgg agaagctgat tgtcgggccg ggagccaaag
    12661 gcaccattga tctgaacctg ccgctggcgg ataacctgcg caatgtagcg gcggcgctcg
    12721 gcaaaccgtt gagcgaactg acggtaacga ttctagctaa accacgccac gatgccgtta
    12781 tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat tccggatggc gacgttgcgg
    12841 cctcaattct cacctgtatg ccagacagcg aagttgacgt gctgtacggt attggtggcg
    12901 cgccggaagg cgtagtttct gcggcggtga tccgcgcatt agatggcgac atgaacggtc
    12961 gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga gaatcgtcgc attggcgagc
    13021 aggagctggc acgctgcaaa gcgatgggca tcgaagccgg taaagtattg cgcctgggcg
    13081 atatggcgcg cagcgataac gtcatcttct ctgccaccgg tattaccaaa ggcgatctgc
    13141 tggaaggcat tagccgcaaa ggcaatatcg cgactaccga aacgctgctg atccgcggca
    13201 agtcacgcac cattcgccgc attcagtcca tccactatct ggatcgcaaa gacccggaaa
    13261 tgcaggtgca catcctctga ttgatttgat cgattgagcc ttccagtcct tcgggactgg
    13321 aatttttttg ttcggagaac gaagataagg caagttaatc aaaacaggag aaaaacatgg
    13381 ctgattgggt aacaggcaaa gtcactaaag tgcagaactg gaccgacgcc ctgtttagtc
    13441 tcaccgttca cgcccccgtg cttccgttta ccgccgggca attcaccaag cttggccttg
    13501 aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt taactcgccc gataatcccg
    13561 atctggagtt ttacctggtc accgtccccg atggcaaatt aagcccacga ttggcggcac
    13621 tgaaaccagg cgatgaagtg caggtggtta gcgaagcagc tggcttcttt gttctggatg
    13681 aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg tacagcgatt ggcccttatt
    13741 tatcgattct gcaactaggc aaagatttag atcgcttcaa aaatctggtc ctggttcacg
    13801 ccgcacgtta tgccgccgac ttaagctatt tgccactgat gcaagaactg gaaaaacgct
    13861 acgaagggaa actgcgcatt cagacggtgg tcagtcggga aacggcagcg gggtcgctca
    13921 ccgggcggat accggcgtta attgaaagtg gggaactgga aagcgcgatt ggcctgccga
    13981 tgaataaaga aaccagccat gtgatgctgt gcggcaatcc acagatggtg cgcgatacac
    14041 aacagttgct gaaagagacc cggcagatga cgaaacattt acgtcgccga ccgggccata
    14101 tgacagcgga gcattactgg taagcggtta cttatcgata aacggcacga tgagcaaatc
    14161 cgcactcatc ttattgatca tcccgcgata tgccggcatc aaacggttga taaatgagtg
    14221 atgatgacca cagacaagga ggtcgcactg ctctttttgc ataatttcca gcagtgtttc
    14281 cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc cattgaatat ttttcgtcag
    14341 tttatacagc ttgttatccg acttattctt caacaattga agaatatctt ctgttgcagg
    14401 gaagtagata cccggataca actcgcttaa gccatcatca atatgaatta acgtcaggtg
    14461 agcgtcatta tgtctggcga gctccagagc tttattcacc agtaaggcat cttcttcatt
    14521 cccggaaatt gccacgccaa tgtgtttata agccatgttt aactccttct aaagcaactc
    14581 catcaagcta acgaacgcag tgataactca aaaataatca tctcggcctg gcatgagaaa
    14641 gtgaaggccg catcaagctc aacttgcccc tccagatcct taatcttgta ggccacgctg
    14701 gccccttcat ctgcagagga tgcagcggcg gcaagctctt tcagtttggt cataaaagac
    14761 tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac tggtacagtt gtcgttatca
    14821 atcaccgtac cgatatcaat cgcacatcct ttagtactgc atttatctac gacatctttc
    14881 atagggcacc tctgtatatt cgcctctctg tcgattacga cttaagaata atgcgccgtt
    14941 aagggaagaa caaacaagta atgatcttat ggattaccat tttttttgat attgcttaag
    15001 gtgaagagtt cagaaggatt ttccgggcat tgtcatttgc atcgtggaaa taacaatccc
    15061 ggaaaaaaat agcagtaaga gatatattaa aggttaatta cttgatttat tgtcggcttt
    15121 atacttcaca tcctgggtat ctttaccata tttattttcg ccttgtgtgc caatgaacgc
    15181 tccaagatcg ataagcatca tcaccagaat caacgtcggg acaaaacgcc ccaccgccca
    15241 ttgccagaca cccggtaaaa tcgcccagtt acccgccagc agcatccacg ccacaatcat
    15301 cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag cgcttaacgg ttactgccgc
    15361 tgttggccaa agcaagcaca caaggcaaaa cgccgcggtc tgaatatcga gtaaattttt
    15421 acccgccagt gagaaaagca ccagcatgcc tgcgaaccac aggcctatcc aaatccagaa
    15481 atcacggcgt ccaatacgcc ctttaaatga gaataaccat tgctgtatgg tcatgtaagt
    15541 tccttgatgg ttgtcttttc caggattcta cccttttgac aagggcgaca gatatcgttt
    15601 taatcggagc cagtcataac aaaaggtact gtcaatgaag ccagggtgta cgctgttttt
    15661 tctcttatgt tctgcattaa ccgttacaac aacggcgcat gcacaaacac cagatacggc
    15721 aacgaccgcg ccttatctgc tggctggagc ccctactttc gatctctcca tcagccagtt
    15781 tcgagaagac tttaacagcc agaatcccag cctgccactg aacgaatttc gtgccatcga
    15841 cagcagtccc gacaaagcca atctcactcg tgctgcaagt aaaattaacg agaacttgta
    15901 tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa agcattcaaa tgacctggct
    15961 acccatccag gggccagagc aaaaagcagc gaaagcgaaa gctcaggaat acatggcagc
    16021 agtgatccgc acactcaccc cattaatgac caaaacacaa agccagaaaa aactgcagtc
    16081 gctgctaacg gcggggaaaa acaaacgtta ttacaccgag acagaaggtg cactgcgtta
    16141 tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct gttgaaccga ttaagctggc
    16201 gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa gcaaagcctt tgtgccgatg
    16261 aatctctata ctgtttcaca gacctgctgc cctgcggggc ggccatcttc ctttattcgc
    16321 ttataagcgt ggagaattaa aatgcgacat cctttagtga tgggtaactg gaaactgaac
    16381 ggcagccgcc acatggttca cgagctggtt tctaacctgc gtaaagagct ggcaggtgtt
    16441 gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca tcgatatggc gaagcgcgaa
    16501 gctgaaggca gccacatcat gctgggtgcg caaaacgtgg acctgaacct gtccggcgca
    16561 ttcaccggtg aaacttctgc cgctatgctg aaagacatcg gcgctcagta catcatcatc
    16621 ggccactctg aacgtcgtac ttaccacaaa gagtctgacg aactgattgc gaaaaaattc
    16681 gcggtgctga aagagcaggg tctgactccg gttctgtgca tcggtgaaac cgaagctgaa
    16741 aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga tcgacgcggt actgaaaact
    16801 cagggtgctg cggcattcga aggtgcggtt atcgcttacg aacctgtatg ggcaatcggt
    16861 actggcaaat ctgcaactcc ggctcaggca caggctgttc acaaattcat ccgtgaccac
    16921 atcgctaaag ttgacgctaa catcgctgaa caagtgatca ttcagtacgg cggctctgta
    16981 aacgcgtcta acgctgcaga actgtttgct cagccagaca tcgacggcgc gctggttggc
    17041 ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta aagctgcaga agcggctaaa
    17101 caggcttaag tctgacaggt gccggatttc atatccggca cttactttcc ttaactcctc
    17161 gccttaacgc aaaatctcac actcatgatc ctggatttcc tcggctgaag cacggttcag
    17221 cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa ttatcactct gccgtaccat
    17281 cgccagcccg tagcttccca tatgttcccg cgcctcaggc acttcttctg caagcatcat
    17341 aaatgggctg cgttgtacca gttcgctttc cgttacccga cgcgccaggt attcatgccc
    17401 gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg ttcgccagat ttttatccag
    17461 ttgttcgcgc acatcaggac gaatacaaga gatatgaata tgaaaatggt tttgcgtacg
    17521 cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg ggaaccggct ggccgtattt
    17581 tttgctcata aaatcacgcg cctgccaggc caaccaaaag aagttcggcg ttgaaggatc
    17641 ggtcaacaaa ggactttcag taccgttaat acgatacgtt ggcatcaaca gatattgcag
    17701 tggaccatta agatctttta aaaccacgta tccggcattg ggtttgactt ccgcacatgg
    17761 cgaaggattt tgattttgct gctgattggg caaacattcc tcaaggacaa tcttacgtaa
    17821 tgtatccgac tcttcaccgg ttaatttcca gtaaccaata ccggcagcca caacggcgat
    17881 aactatcatc accaaaaaaa gaagacccgc ttttttcatc tttttttccc tgtacctcaa
    17941 agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa cccggtcgcg agaaaccacc
    18001 gggtttgata attatcctgg ggaaatcagc gtttgctgat ctgatcgaac gtaccgccgt
    18061 tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa atcttcatca atggtgaata
    18121 acttcagctt tggaaacgca ttttcgtact ttttcgctac ttcagcgtcg cgcggacggt
    18181 agtagttttt cgcggcaatt tcctgacctt ctggcgagta gagatatttc aggtaggctt
    18241 ccgccacctc tttggtgcct tttttctcga ccactttatc gaccaccgac acggttggct
    18301 ctgcgaggat agactcactc ggcgtgacga tttcgaattt atctttcccc agttcattcg
    18361 ctgccagcag agcttcgttt tcccaggcga tcagtacatc gccaatcccg cgctcgacaa
    18421 aggtgttagt ggagccgcgc gcgccagaat ccagaacttc gacgttttta tacagtgccc
    18481 gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg atgcagcgcg tagccccagg
    18541 cagccaggta gttccagcgt gcgccaccag agcttttcgg attaggcgtg atcaccgaaa
    18601 cacccggttt aatcagatcg ttccagtcat ggatctgctt cggattgccc ttacgcacca
    18661 ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg cagacgtttg atccactctt
    18721 tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata ggccagagcc agcgtgacaa
    18781 catcagcttc aataccgttg attaccgacg tcgcttgttt acctgaacca ccgtgtgact
    18841 gacgaatcac cacgttatca ccagtttgct gtttccagtg ggcgctgaat gccttgttgt
    18901 actgttcgta caattcgcgc gttggatcat atgaaacgtt aagaagctga atatcctttg
    18961 ccataacgct ggttgccgcc agcaaaaatg ttaaccctac gccccacttg ttcatcgccc
    19021 gactctctta tgttgtgttg tgatgagcaa agcgtgccag aaggttaacc aaacattaaa
    19081 gaataaaaaa agattggcta taacttgcgg gtatatgttg ggggttaaaa aggcggaaga
    19141 aatccgcctc atattgctga caaagtgcgc tttatcgatg ccggatgcgg cgcgaacgcc
    19201 ttatccggcc tacaaaaatt tgtaaattca ataaattgca ga
//
LOCUS       DAZFRN010000090        18886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFRN010000090 DAZFRN010000000
VERSION     DAZFRN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.090"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            180..386
                     /locus_tag="ACHKUB_003077"
     CDS             180..386
                     /locus_tag="ACHKUB_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656057.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            939..1433
                     /locus_tag="ACHKUB_003078"
     CDS             939..1433
                     /locus_tag="ACHKUB_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO6656058.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1433..3535
                     /locus_tag="ACHKUB_003079"
     CDS             1433..3535
                     /locus_tag="ACHKUB_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6656059.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPCCSGRILPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPTPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            3532..3744
                     /locus_tag="ACHKUB_003080"
     CDS             3532..3744
                     /locus_tag="ACHKUB_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656060.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3672..5252
                     /locus_tag="ACHKUB_003081"
     CDS             3672..5252
                     /locus_tag="ACHKUB_003081"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6656061.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKENERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDGY
                     LILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKE
                     AEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGS
                     SAATKRQEPQHTDDQSEE"
     gene            5197..7224
                     /locus_tag="ACHKUB_003082"
     CDS             5197..7224
                     /locus_tag="ACHKUB_003082"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO6656062.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSGYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7311..7634
                     /locus_tag="ACHKUB_003083"
     CDS             7311..7634
                     /locus_tag="ACHKUB_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO6656063.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLANSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7627..7902
                     /locus_tag="ACHKUB_003084"
     CDS             7627..7902
                     /locus_tag="ACHKUB_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO6656064.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7914..8492
                     /locus_tag="ACHKUB_003085"
     CDS             7914..8492
                     /locus_tag="ACHKUB_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6656065.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKVPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8489..8890
                     /locus_tag="ACHKUB_003086"
     CDS             8489..8890
                     /locus_tag="ACHKUB_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6656066.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8902..9645
                     /locus_tag="ACHKUB_003087"
     CDS             8902..9645
                     /locus_tag="ACHKUB_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6656067.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVAAVAVSAAG"
     gene            9706..10092
                     /locus_tag="ACHKUB_003088"
     CDS             9706..10092
                     /locus_tag="ACHKUB_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6656068.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGVIENAGDLEHEVLTPEKS"
     gene            10101..10430
                     /locus_tag="ACHKUB_003089"
     CDS             10101..10430
                     /locus_tag="ACHKUB_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6656069.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10402..13467
                     /locus_tag="ACHKUB_003090"
     CDS             10402..13467
                     /locus_tag="ACHKUB_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6656070.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAASVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTREPRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13467..13796
                     /locus_tag="ACHKUB_003091"
     CDS             13467..13796
                     /locus_tag="ACHKUB_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6656071.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSIY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            13806..14504
                     /locus_tag="ACHKUB_003092"
     CDS             13806..14504
                     /locus_tag="ACHKUB_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6656072.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14510..15253
                     /locus_tag="ACHKUB_003093"
     CDS             14510..15253
                     /locus_tag="ACHKUB_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6656073.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLSAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEDT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLVAVSTFV"
     gene            15151..15798
                     /locus_tag="ACHKUB_003094"
     CDS             15151..15798
                     /locus_tag="ACHKUB_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6656074.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPVFRQKLNEGWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15859..>18886
                     /locus_tag="ACHKUB_003095"
     CDS             15859..>18886
                     /locus_tag="ACHKUB_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6656075.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQGYPNTALV
                     GVKVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTEITYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTS"
BASE COUNT         4502 a         4455 c         5786 g         4143 t
ORIGIN      
        1 atctcgattg tcgatggtat tggttgtgtg acaataccta gtctttccgg tatgcctgaa
       61 gagaatacaa acgacagatt atgtaaggga aacgcgctga aactttcact ctttcatgct
      121 gagtgatttg acgttatatt tttagccgca agtgaagagc aaacgaatgg agcgacaaaa
      181 tgcataaaga tcaatacact gatgccccct cccaggagca ggttcgtgta aaaactatgc
      241 ttaatagcac catttcgatg ggttacccgg atgttgtaat tgcatgtata gaacatcaag
      301 tatctctgga agcattcagg gcaattgagg ccgcgttagt aaagcacgat aagaatacga
      361 aggattattc cctggtggtt gactgatcac cataactgct aaccattctg accatttcac
      421 aagtgacaga gtcaatatcg tattctgtca ctgtaaggct aatacagtac tgcaattcaa
      481 ctacagcaat gcctcgtatt taggtgaatt tacaatatcg tccagttcgg atgccggctg
      541 catttctgaa gataaggcat ttcggttcgc gtattttccc ctcatgctcg tcagtcctgc
      601 gcgtagtaag aaacaggaca ctcacactaa tttgtgtggg catgctgtga tgtccttctg
      661 aattatcccc atgccattat gtaacgctct gtttcagatg ctcgtcacgg ttgtcaggct
      721 gtcgggtcct cccagtgggg gtccctgcca cggggcggga gcgtcgcgga aaaaggctag
      781 tttttgaaat tttattcgtc atcaccacta ctgtaattga ttgatattac agtgatttta
      841 ttttcatggt gtcgattctg attgtttttt gttcatcact aacaccgttt gcctaaagtt
      901 gttcgcaaga tgcatgttta aaacattctg gagcgggtat ggatcgagag ttaaaaaatc
      961 tgacgctgaa tatcagtcaa ctggcggcat tgtcaggtgt acatcgccag actgctgcgg
     1021 caaggctgca aaatctaccc gttgcagggg ggcatgaaag caacctcaag ctttatcggg
     1081 tggttgatat tgtgtcggca tttctggcat taccaccgcc ggttgcagaa ggcgaaatgg
     1141 acgcgcatga gcgcaaagcc tggtatcagt ctgaacgtga gcgtcttaag ttcgaacagg
     1201 aaacggcaca actcattccg gccagtgatg tcagacggga atttgccatc tgggcaaaag
     1261 cggtcgtgca ggtgctggag acattaccgg atattcttga acgtgactgc ggtctgcagc
     1321 ctgccgctgt gagccgtgtt cagtccatta ttgatgatct gcgcgatcag atagccctgc
     1381 gggtgactga agcaggtgcg gatgatgagg aggaattaca gcaggaggag taatgctgaa
     1441 tcaggaaacc gcaaaggcag cacgaaccga ttcaggttat atccttcgcg caccgagacg
     1501 aatgcgggtt gctgatgccg ttgctcagta tatgcgggtg cctatggggg cagggaactc
     1561 agtcccgtgg gatccgctgg tggcaccgta tgttattgag cctatgaact gcctggcctc
     1621 gcgtgaatac gacgcagtga tatttgttgg cccggcacga accggcaaga ctatcggcct
     1681 gattgacggc tgggtgattt acaacgtgat ttgcgatcct gctgatatgc tgatcattca
     1741 gatgacggag gaaaaagccc gcgaacactc caaaaaacga ctcgccagaa cgtttcgcgt
     1801 cagcccggaa gtggtcagtc gcctgagtcc gaacaaaaat gacaacaacg tttatgacag
     1861 aacattcctt gctggcaact acctgaaaat cggctggccg tcagtcaata tcatgtcttc
     1921 atcagattat aaatgcgtgg cgctgacgga ttatgaccgt tttccggaag atattgatgg
     1981 tgagggggat gccttctctc ttgcctcaaa acgtaccacc acatttatgt ccagtggtat
     2041 gacgctggtg gagagttccc ccggcaggga tgtgaaggat gtgaaatggc gacggacttc
     2101 accgcatgag gctccaccaa ccacggggat cctgtcgctc tataaccgtg gcgatcgccg
     2161 tcgctggtac tggccctgtc cacactgtgg tgagtatttt cagccctgcg gcgatgtggt
     2221 tgctggtttc cgtgatattg ccgatcctgt gctggcaagt gaggcggctt atattcagtg
     2281 tccttgctgt tcaggacgga ttttgcctga acaaaaacgc gagctgaacg gacgtggggt
     2341 ctggttacgg gatggtgaat ccatcaatgc agatggcagt cgttatggtg atccccggcg
     2401 ttcacgtatt gcgtcattct ggatggaggg tccggcagct gcttatcaga cactctcgca
     2461 actcgtttac aaactgctta ctgcagaaca ggaatacgag acaaccggaa gtgaagagac
     2521 actcaagacg gttatcaata ccgactgggg attaccttat cttccccgcg ccagcatgga
     2581 gcaacgaaaa agtgaattgc ttgagcagcg ggcagagcca gttccttccc gcagtgtgcc
     2641 ggatggcgtt aatttccttg tggcgacagt ggatgtgcag gcgggacgtc atcgccgttt
     2701 tgtggttcag gtaacgggct atggcagccg tggcgaacgc tggattattg atcgttacaa
     2761 catcacgcag tcattgcgcg gtgacagcga cggggagagc cagcgaattg atccggccag
     2821 ctatccggaa gactgggatg tcctgctgac ggatgttttt cataaaagct ggccgctggc
     2881 ctccgatcct tctcaacaaa tgcgactgat ggcaatggcg gtggactccg gcggtgaaga
     2941 cggggtcact gataatgcct ataaattctg gcgtcgttgc cgtcgtgatg gccttggtaa
     3001 acgtatttac ctgtttaagg gtgacagcat ccggcgcgca aaactgatca gccgtacatt
     3061 ccctgataac accggacgaa cgggccgccg ggcgcaggcc gcaggtgatg tgccgctctg
     3121 gcttcttcag acggatgccc tgaaagaccg ggtgaataac gcgttatggc gtgactcgcc
     3181 aggtcccggc tatgtgcatt tccctgactg gctggggagc tggttttacg acgaactgac
     3241 gtatgaagag cggagcagtg acgggaaatg gagtaagccg ggtcgcggtg ccaacgaagc
     3301 ttttgacctg atggtgtatg ccgaggctct ggtcattctg catggatacg aaaagatccg
     3361 ctggccggat gcaccggagt gggcgagccg ggaaacctgg ctggagtgtg tcccggacag
     3421 taccgaaccg tcacccacac cggaaccggt atccacgcct gttaaaaaac aaaaacggaa
     3481 gaaaacagta actgacgatg ttaacccctg gctgacttcc ggaggatggt tatgaaccag
     3541 aatgatatcg aagccatgat tcagcgttat acggaagctg aaatggcggt gctggacgga
     3601 aaatccgtca cctttaatgg tcagcagatg accatggaaa acttatctga gatccggcag
     3661 ggacggcagg agtgggagcg ccgccttgcg gctctgatta cacgacgacg ggggcatccc
     3721 gggtaccggc tggcgaggtt ctgatggcaa ttcttgatga tgtgattggc gttttttcac
     3781 caggatggaa agcggcaagg ctgcgttccc gtgcggtgat ccaggcttat gaggccgtaa
     3841 aaacgacacg gacacacaaa gcccggcggg aaaaccgaac tgccgaccag ttaagccagt
     3901 acggggccgt gtcgttacgt gagcaggccc gttaccttga taacaaccac gatctggtta
     3961 ttggtgtatt tgacaagctg gaagaacggg tggtggggaa aaacgggatt attgtcgagc
     4021 cacatccggt attacgcaat ggggccattg cccgtgatct ggcagcggag atacgcaccc
     4081 gatggagtga atggtctgtc agtccggaag tcaccgggca gtttacccgt ccgatgctgg
     4141 aacgtctgat gctgcgtacc tggctgcgcg atggtgaggt gtttgcccag atggtttccg
     4201 ggcgcataaa cagcctgacg ccttctgccg gtgttcattt ctggctggag gcgctcgagc
     4261 cggactttat tcccatgacc agtgatgaga gcaacaggct gaatcagggc gtgtttgttg
     4321 atgactgggg gcgtcccgaa aaatatctgg tgtataaaag ccgtcccgta tccgggcggc
     4381 agatggaaac caaagaagtg gatgcagagc gaatgctgca tcttaaattt gttcgccgtc
     4441 tgcaccagat gcgcgggacg tctttgttgt ccggtgtgct gatccgcctc agtgccctga
     4501 aagagtatga agattctgag ctgactgcag caaggatcgc cgctgctctg gggatgtaca
     4561 tccggaaagg cgacgggcag agctatgaac cggatggtaa tggcagcaag gagaatgaac
     4621 gcgagcttac cattcagcca ggcattattt acgacgatct gaaacccggc gaagaaatcg
     4681 gaatggtgaa gtcggatcgt cccaatccta accttgaaac ttttcgtaat ggtcagttgc
     4741 gtgccgtggc ggcgggcagt cgtctgagtt tttccagtac ggcacgcaac tataacggca
     4801 cttacagcgc ccagcgtcag gagctggttg aatccactga tggctacctg atcctgcagg
     4861 actggtttat tggtgccgtc acccgcccga tgtatcgtgc ctggctgaaa caggctgtgg
     4921 catccggtgt tatcaggcta ccccgcgatc ttgaccgttc ttcactgtat accgcggtgt
     4981 attccggacc ggtgatgccg tggattgacc ctgttaagga ggctgaggcc tggaaaattc
     5041 agattcgtgg tggagcggcg acagaatcag actgggtacg tgcaggtggt cgtaatccgg
     5101 atgatgtcaa acgtcggcgc aaggccgaaa ttgatgaaaa ccgcaagctg gatctggtat
     5161 ttgataccga tccggccagt gataaaggag gcagcagtgc cgcaacgaaa cgacaggagc
     5221 cgcagcacac cgacgaccag tccgaagaat aattcctggt tcaggatgca ggcaggtcac
     5281 cagagtgacg cggatattta tatttatgac gagattggtt tctggggtgt tacagcgaag
     5341 cagtttatca gtgatctgaa tgcactgggc gatatcaccc acattaatct ccatattaat
     5401 tcaccgggtg gcgatgtctt tgaaggcatc gccattttta atgcactgaa aacacatggt
     5461 gcgtccatta ccgtttatgt cgacggtgtg gcggcgtcaa tggcgtcggt cattgcgatg
     5521 gtgggaaacc cggtcattat gccggaaaac tccttcatga tgattcataa accatttggc
     5581 tttacgggcg gtgatgcgga ggacatgcgc acctatgccg acctgctcga taaagttgag
     5641 gcggttctgt tacccgctta tgcacagaaa accgggaaaa ccaccgatga aattgctgcc
     5701 atgctggcgg atgagacctg gatgtccggt gccgaatgtc tggcacaggg atttgctgat
     5761 caggtgacac cagccgttaa ggcaatggca tgtattcagt caaaacgtac agaggaattt
     5821 aaaaagatgc cggaatccat tcgaaacatg attactccgc cacgcaacag tgctccacgc
     5881 gtacaggatg atgaacctgc agcctcccgg acgccagtgc aggcagcagc acccgtggtg
     5941 gatgaaaaca gtatccgtgc gcaggtactg gcagagcaaa aagcgcgtgt aaacggtatt
     6001 aatgatctgt ttgccatgtt tggcgggcgt tatcagacgc tgcaggccca gtgtcttgcc
     6061 gatcctgaat gttcgctgga gcaggcccgt gaaaagctgt tgaacgagat ggggcgcgag
     6121 tccacgccat ccaataaaaa taccccggct catatttatg ccggtaacgg taattttgtg
     6181 ggggacggga ttcgccaggc gctgatggcg cgtgccggat ttgaaaaaac cgaacgtgat
     6241 aatgtctaca acgggatgac cctgcgtgaa tatgcccgta tgtcactaac tgaacggggt
     6301 attggggttt ccggttataa cccgatgcag atggtcggtg cggcgttcac acacagtacg
     6361 tctgacttcg gtaatattct gctggatgtt gcgaacaaag ccattctgca gggctgggaa
     6421 gatgcccctg aaacctatga acagtggacg cggaaaggtc agttgtctga ttttaaaatt
     6481 gcccatcgtg tgggtatggg gggcttcagt gctctacgtc aggtgcgtga aggggcggaa
     6541 tataaatacg tcaccaccgg agataaacag gccactattg cactggcgac ctatggcgag
     6601 ctgttcagta tcacccgtca ggccattatc aatgatgatc tgaatatgct gaccgatgtc
     6661 ccgatgaaac tgggccgtgc ggcgaaatcc actattgccg atctggttta tgccattctg
     6721 acgtctaacc cgaaaatctc cacagataat gtaagtctgt tcgataaagc gaaacatgca
     6781 aacgtactgg agagcgctgc aatggacgtg gcatcgctgg ataaagcccg ccagttgatg
     6841 cgcgttcaga aagaggggga gcgtcatctg aatattcgtc ctgcgttcgt actggtaccg
     6901 acggcgatgg agtctgttgc taaccaggtc attcgctcct caagtgtcaa gggggctgac
     6961 attaacgccg gtattattaa cccggtgaaa gattttgcga ccgttattgc agagcctcgt
     7021 cttgatgata acagccagac caccttctac ctggctgcgt caaaaggctc cgatacgatt
     7081 gaagtggctt atctcaacgg tgtggatacg ccatatattg atcagatgga gggcttcagt
     7141 gtggatggcg tgacaacgaa agtgcgtatt gacgccggtg tcgcgccagt tgatcaccgc
     7201 ggtctggtga aatgtacggc gtaaacgtcg cagacaacaa ctctgatggc ccgtaagggc
     7261 tttttttgta cctgaaatca gcccctgaac ggggctgtgc ggagacagtt atggcaaaga
     7321 attttgtaga agaaggaaaa acggtggcga ttgttgccag tgcagccatc agcagcggag
     7381 atctggtgca ggtgggcgat gtttttgcgg tggcgctgac cgatattcca cagggtgaaa
     7441 caggcgacgg catgaccgaa ggtgtgttta tgctgcctaa gctgaaaacg gatgacatga
     7501 aaacgggtaa gaaggtttat ctgaagtccg gaaaagttca gctggctaac agcggctctg
     7561 atccgctggt cggggttgtc tgggcagatg ccggaaccag tgcagaagaa gtgccggtaa
     7621 aactcaatgt ctgatccctt ttcccggctg gcagcgcgta tggatgctat cacggtcaga
     7681 aagatgggaa agacagcctc gattaatgat gtcgatatga ctgtgatccc gggagaaaca
     7741 ctggcagagc tgaatgctct gtccggacct gcggtctctc tggtggtgtt ttcttcggga
     7801 taccgcccac ggcgcgggga tcgcgttgtt tatgacggac aacaatggac ggtcacacgg
     7861 catgaacgtt ttaacggtaa gccaatgatc tttattgagt aaagaggtgt gggatgaagg
     7921 ggcttgagaa tgccatccgt aatctgaaca gccttgatac ccgtatggtg ccacaggcca
     7981 gcgcatgggc gataaaccgt gtggcacaga aagcggtctc ggtcgccacc cggcaggttg
     8041 ccgggaatac cgttgcggga gataaccagg tgaaagggat ccccctgaaa ctggtacgtc
     8101 agcgtgtccg ggtgtttaaa gccagtccgt caggaaaaat gacggccagg atccgcgtta
     8161 accggggcaa tctgcccgct attaagctgg ggacagcccg ggtcagactg gcccggcgtg
     8221 gtggaaaact gcagtaccgt ggcagtgtgc tgaaggtggg taaatatctt ttccgggatg
     8281 cgtttattca gcaactggcg aatggtcgct ggcatgtgat gcggcgtatt gatggcaaaa
     8341 atcgttaccc cattgatgtg gtgaaagtcc cgctgtccgg accgctgaca caggcatttg
     8401 aagatgcccg cgaccgcatt attgctgcgg aaatgccgaa acagctgggg tatgcactga
     8461 aacaacaact gaggttatgg ctgacccgat gaaccgacat acacaaatcc gccaggccgt
     8521 actggcacgc cttcgggaac agtgtggaga cagcgccacg ttttttgacg ggcttccggc
     8581 atttattgat gcgcaggaac tgcctgccgt ggcggtgtgg ctgagtgatg ctcagtacac
     8641 cggaaaaatg acggatgaag atgactggca ggctgttctg catattgctg tcttcatccg
     8701 ggcacaggca ccggattcag agctggatat gtggatggag agcaccattt tcccggccct
     8761 gaatgatata ccggcacttt ccggactcat cgacaccctg atccctctcg gttttaacta
     8821 tcaacgtgat aatgagatgg ccacctgggc gatggcggaa atcacgtacc agatcacgta
     8881 cacgaattaa aggaggtggc aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg
     8941 ggaacgacgt tctggatgta caccggcaag ggcgatgcgt ttgcgaaccc tttatcggac
     9001 actgactggc tgcgtcttgc gatggtgaag gatctgcaac ctggcgaaat gaccgctgat
     9061 gcagaagatg acacttatct cgatgatgaa gatgcagact ggaaaacgac aacccagggg
     9121 cagaaatccg tcggtgatac ttcggcgacg ctggcctggc gtccgggtga cagcgggcag
     9181 aaaaaactgg ttcagttgtt cgactccggt gaagtctgcg cgtttcgtat caaatatccc
     9241 aacggcactg ttgatgtttt ccgtggctgg ctgagctcac tgggtaaaac cattgcctca
     9301 aaagacgtga tgacccgcac agtgaaaatc agcggtgtgg ggcgtccgta tctggcagag
     9361 gaaggcactg aaacagtgag cgttaccggg ctgacggtgg caccggcatc tgccagtgta
     9421 aaagtgggag caaccaccac gctgaccttt acagtaaaac ctgacggagc cagtgacaaa
     9481 gcgatcagtg tgcattcgac agatccacag actgccactg tgaccctgaa cgggcttgtg
     9541 gccacggtga aaggcgtgaa gcagggcagt gtcagcattg tgggcatgac ttctgacggc
     9601 gattttgtgg cagtggctgc ggtggctgtc agcgccgcag gttaacagga cgatactcat
     9661 catttgcccc ggttatccgg ggcttttttg caggtggaga acatgatgtt tctgaaacag
     9721 ggcacgttta attatgaaaa gcagtccgtg gtgctcagtg agctgtccgg gctgcagaga
     9781 attgaatatc tggcgtttgt tcagcagcga acggcaaagt ttgatgccga agagggagaa
     9841 ctgccggagg ctgaacgaca gattgctttt ctgcggatgg ggatggatat caatgcctgg
     9901 ctggtttccc gctcactgtg gaatgcggaa cagtctcagg atgttgagac gctttgcgca
     9961 tccgttatta caacatggtc gtatgatgcc ctgggagcgg gggcggagat ggttctgtcg
    10021 ctgagcggta tgggagtcat tgagaatgcc ggggatttgg agcatgaggt gctgacgccg
    10081 gaaaagtcct gacgcgggaa atgcagtttg tcatgcggct tgcccgggag ttccggcggg
    10141 cagactggcg gcggatgctg tcggaaatgt cggccactga gcttggtgag tggggcgatt
    10201 atttccggat gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg
    10261 cattgatcgt gagaatggtg tccggtagca gtgatgctgc ggtggctgat ttcagccttt
    10321 taccggaaga gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag
    10381 gtatttccgg aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt
    10441 gatgcggcag agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc
    10501 agcgatgcag aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcc
    10561 gcccggcaga caatgcaggc ggcttcttcg gctgcaacag ccgcatccgt ccatgcgcag
    10621 acggtggaga agagcgcaca ggctcatgaa cgcatggccc gcgaggtgga gcaaacccgc
    10681 cagcgtatgg aggcactgag ccagaaaatg cgcgaggaac aggcgcaggc catggctctg
    10741 gcggaggctc aggataaagc ggctgccgcg ttttatcgtc agattgacag tgtgaaacag
    10801 gccagtgcgg ggctgcagga attacagcgt attcagcagc agatccgaca ggccagaaac
    10861 agtggcggga ttggtcagca ggattatctg gcgctgattt ctgaggttac ggcgaaaacc
    10921 cgtgttctta cgcaggctga ggaagaggct acccgacaga aagtggcgtt tatccgtcag
    10981 cttaaagagc aggcaacccg ccagaatctt tcttcttctg agttgcttcg tgctaaggct
    11041 gcccagttgg gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcaggcagga
    11101 aaagccacgc attcgctggg tctgaaaagt gcagcggccc gccaggagat tggcgttctg
    11161 ataggtgaac tggcccgcgg caatttaggg gcgctgaggg gatccgggat aacgctggct
    11221 aaccgtgccg gatggataga cactctgatg tcaccgaaag gcatgatgct tggcggggtt
    11281 attggcggta ttgccgcgtc cgtctatggt ctgggtaaag cctggtatga cggtcagaag
    11341 gagggggaag aatttaaccg ccagttgtcg ctgacggggc attatgccgg agtcactgcc
    11401 gggcagctgt ggacgctcag tcgtgctatt tccgggaatg gtatcacgca acatgctgca
    11461 gccggtgcgc tggctcaggt ggtggggagt ggtgcatttc gtggaaacga tatcggtatg
    11521 gtggcgagag ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata
    11581 aatcagttta agcggctgaa ggatgatcct gtaaatgccg cgaaggctct ggacaatgag
    11641 ctgcattttc ttactgccac tcagcttgag cagatacgtg tccttgggga tcagggacgg
    11701 tccagtgatg ctgcacggat agccatgtct gcactggcag aggaaaccgg tcggcgtact
    11761 gcggatattg ataataacct caatgcgctt ggcagtacgc tgaagtatct gtctgatttg
    11821 tggagtcgtt tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgaacag
    11881 attgccgctt tacaggagaa agtgtcgcgg gcgaaaagac tcccctggac ggcatcatct
    11941 tctcaggttg aatacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccagaag
    12001 gatttgcagg atgcaaaaga gcaggcagag cggaattatc aggagcaaca gaaacgacgt
    12061 aatgctgaaa atgctgcact gaaccggatg aatgaaacgg aagcagcacg acatcagcgt
    12121 gaaattgcgc gtattaattc catgcagtac gccgatcagg ctgtcaggga tgcagcgata
    12181 caacgtgaaa atgaacgtta cgagaaagcc ttggcatccg gtaagaaaaa aacacgcgaa
    12241 ccccgtaatg atgaggccac ccggttattg ctgcagtaca gtcagcaaca ggcacaggtg
    12301 gaaggacaga ttgctgctgc cagacagtca gcaggcattg ccacggaaag gatgacagaa
    12361 gcgcataaac agcttctggc tctgcagcag cgcatcagcg acctggacgg gaaaaaactg
    12421 acggcagatg aaaagagtgt gctggcccgt aaagatgaac tgattcaggc actgacgctg
    12481 ctggatgtaa aacagcagga gcttcagaaa cagacggcac tcaacgagct gaagaaaaaa
    12541 acaattcagc tgaccagtca actggctgaa gaagagcgcg ctcagcgtca gcaacatgac
    12601 ctggatatcg ccacggtggg tatgggtgat cagcagcggc agcgatatca ggtacaactg
    12661 agtcttcgcc agaaatacca gcaacagctg gagcagttga ggcgggatag tgagcagaaa
    12721 ggaacatata acacggatga ctacagaaag gccgagcagg cgctgacgga gagcctgaac
    12781 cgacaactga atgagaatcg ccgttactgg caacagcttg aagttgtgca gggtaactgg
    12841 aaaaacggag tcctgcgtgc atttcaggat tttaccgtgg atgcagataa tacggcagga
    12901 acagcagaac aggtgttctc gtcagccttc agcaacatgg gaaatggcct ggcaactttt
    12961 gtcactaccg gcaaactcaa tttcaaatcc ttcacctctt ctgtgctgtc agatatggcg
    13021 aaaatcctgg cgcaggcaac catgatgaaa tcgataaaag ggattggcag tgtgctggga
    13081 tttgatctca gcagcctttc cctgaatgcc aatgggggga tttatcagtc tgctgatttg
    13141 agtcgttaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc aaaaggcgcg
    13201 ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgtgg tgctgacggt
    13261 aagctggggg ttgtggcgga tattggtggt tcaggtatgg cgatgtttgc cccgcagtac
    13321 aacatcgaga tcaataacga tggcacgaac gggcagatag gtccggctgc cctgaaggtg
    13381 gtttatgacc ttgggaaaaa agcggcagcg gactttatgc aacagcaggc ccgtgatggt
    13441 ggtcggttaa gtggagcata tcggtaatgg agacgtttca ctggaaagtg cgcccggata
    13501 tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt tatgaacagc
    13561 gtcgtgcggc gggactgaat aaccagttgt cgatttacag cgtgacgata cgtgttcgta
    13621 aatgtgaaca cccatcttta aaagcctttc tggaacggca cggtggcgtc cgcgcatttc
    13681 agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa tggtcggcaa
    13741 gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg gcataggagg
    13801 ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc actcagtcag
    13861 cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt tattttttct
    13921 gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag tatcaggcat
    13981 accccattca ggggacggga tttgaactga atggcaaggg cagtgctgcc cgtccgacac
    14041 tgacggtttc taacctgcac ggcatggtca ccgggatggc ggaagatctg cagagtctgg
    14101 tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg
    14161 tcaacggaaa cagtgacgcc gatccggagc aggaggtgat cagccgctgg cgcatcgagc
    14221 agtgcagcga actgagcgcg gtgagtgcct cttttgtact gtccacgccg acggaaacgg
    14281 acggtgccgt ttttccgggg cgcatcatgc tggccaacac ctgcacctgg acctatcgcg
    14341 gtgacgagtg cggttatcac ggtccggcgg tcgcggatga atatgatcag ccgacgtccg
    14401 atatcacgaa ggataaatgc agcaaatgcc tgagcggctg taagtttcgc aataatgtcg
    14461 gcaactttgg cggcttcctt tccattaaca aactttcgca gtgaatccca tgacagagac
    14521 agaatcagcg attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gcggcttcgt
    14581 ggtgagaaca ccggaggggg aaagatattt tccctgcgtg aatatctctg gtgagccgga
    14641 ggcgtatttc cggatgtcgc cggaggactg gctgagtgca gaaatgcagg gagagattgt
    14701 ggcgctggtc cacagccacc ccggtggtct gccctggctg agtgaggccg accggcggct
    14761 gcaggtgcag agtgatttgc cgtggtggct ggtctgccgg ggggcgattc ataaattccg
    14821 ctgtgtgccg catctcaccg ggcggcgctt tgagcacggg gtgacggact gttacacgct
    14881 gttccgggac gcttaccatc tggcgggaat tgagatgccg gattttcatc gcggggatga
    14941 ctggtggcgt cacggccaga atctctatct ggataatctg gaggacacag ggctgtatca
    15001 ggtgccgttg tcagcggcgc agccgggcga tgtgctgctg tgctgttttg gttcatcggt
    15061 gccgaatcat gccgccattt actgtggcga cggcgagctg ctgcaccata ttcctgaaca
    15121 actgagtaaa cgagagaggt acaccgacaa atggcagcga cgcacacact ccctctggcg
    15181 tcaccgggca tggcacgcat ctgcctttac ggggatttgc aacgatttgg tcgccgtatc
    15241 gaccttcgtg tgaaaacggg ggctgaagcc atccgggcac tggccacaca gctcccggtg
    15301 tttcgtcaga aactgaatga gggctggtat caggtgcgca ttgccgggcg tgatgcaggc
    15361 gaaaatgaat tatctgcccg tcttaatgag ccgctggcaa atggtgccgt gatccacatc
    15421 gtgccgcgtc tggcgggagc taaaagtggc ggtgtgtttc aggtggtgct tggggcggcg
    15481 ctgattgcgg tggcatggtg gaaccctgtg ggctggctgg gggccgcggc tgtatcgggc
    15541 atgtatgcgg caggggccag tatgatcttg ggcggagtgg cgcagatgct ggcaccgaaa
    15601 gccaggacgc ccacggcagc cagtacagat aacggcaaac agaacaccta tttctcctca
    15661 ctggataaca tggttgccca gggcaatgtt ctgcctgttc tgtacggtga aatgcgcgtg
    15721 gggtcacgtg tggtttctca ggagatcagc acggcagacg aaggggatgg tggtcaggtt
    15781 gtggtgattg gtcgctgatg caaaatgttt tatgtgaaac cgcctccggg cggttttatc
    15841 gtttatggag cgtgaggaat gggtaaaggc agcagtaagg ggcatacccc gcgcgaagcg
    15901 aaggacaacc tgaagtccac gcagctgctg agtgtgatcg atgccatcag cgaagggccg
    15961 gttgaaggtc cggtggatgg attaaaaagc gtgctgctga acagtacgcc ggtgctggac
    16021 agtgagggga ataccaatat atccggcgtc acggtggtgt tccgggccgg tgagcaggag
    16081 cagacaccgc cggagggatt tgaatcctcc ggctccgaga cggtgctcgg tacagaagtg
    16141 aaatatgaca cgccgatcac ccgcgccatc acgtcggcaa acatcgaccg actgcgcttt
    16201 accttcggtg tgcaggcact ggtggaaacc acctcaaagg gggaccggaa tccgtcggaa
    16261 gttcgcctgc tggttcagat acagcgtaat ggtggctggg tgacggaaaa agacatcacc
    16321 attaagggca aaaccacctc gcagtatctg gcctcggtgg tggtggataa cctgccgccg
    16381 cgcccgttta atatccggat gcgcagaatg acgccggaca gcaccacaga ccagctgcag
    16441 aacaaaacgc tctggtcgtc atacaccgaa atcatcgatg tgaaacaggg ctacccgaac
    16501 acggcactgg tcggcgtaaa ggtggattcg gagcagttcg gcagccagca ggtgagccgt
    16561 aattatcatc ttcgcgggcg tattctgcag gtgccgtcga actataaccc gcagacgcgg
    16621 caatacagcg gtatctggga cggaacgtta aaaccggcat acagcaacaa cccggcctgg
    16681 tgtctgtggg atatgctgac ccacccgcgc tacggcatgg ggaaacgtct tggtgcggcg
    16741 gatgtggata aatgggcgct gtatgtcatc ggccagtact gcgaccagtc ggtgccggac
    16801 ggctttggcg gcacggagcc gcgcattacc tgtaatgcgt acctgaccac acagcgtaag
    16861 gcgtgggatg tgctcagtga tttctgctcg gcgatgcgct gtatgccggt atggaacggg
    16921 cagacgctga cgttcgtgca ggaccgacca tcagataagg tgtggaccta taaccgcagt
    16981 aatgtggtga tgccggatga tggcgcgccg ttccgctaca gcttcagcgc cctgaaggac
    17041 cgccataatg ccgttgaggt gaactggatt gacccggata acggctggga gacggcgaca
    17101 gagcttgtgg aggacacgca ggccattgcc cgttacggtc gtaatgtcac gaagatggat
    17161 gcctttggtt gtaccagtcg ggggcaggca caccgagccg ggctgtggct gattaaaacg
    17221 gaactgctgg aaacgcagac cgtggatttc agcgtgggcg cagaagggct tcgccatgtg
    17281 ccgggcgatg ttattgaaat ctgcgatgat gactatgccg gtatcagcat cggtggtcgc
    17341 gtgctggcgg tgaacagcca gacccggacg ctgacgctcg accgtgaaat cacgctgcca
    17401 tcctccggca ccacgctgat aagcctggtt gacgggcagg gtaatccggt cagcgtggag
    17461 gtccagtccg tcaccgacgg cgtgaaggtg aaagtgagcc gtgttcctga cggcgttgct
    17521 gaatacagcg tatgggggct gaagttgccg acgttgcgcc agcgcctgtt ccgctgcgtg
    17581 agtatccgtg agaacgacga cggcacgtat gccatcaccg ccgtgcagca tgtacccgaa
    17641 aaagaagcca tcgtggataa cggggcgcac tttgacggcg accagagcgg cacggtgaat
    17701 ggcgtcacgc cgcccgcggt gcagcacctg actgccgaag tcaccgcaga cagcggggaa
    17761 tatcaggtgc tggcgcgctg ggacacgccg aaggtggtga agggcgtgag ctttatgctt
    17821 cgcctgaccg tggcagcgga tgacggcagt gagcggctgg tcagcacggc ccggacgacg
    17881 gaaatcacat accgcttcac gcaactggcg ctggggaact acaggctgac agtccgggcg
    17941 gcaaatgcct gggggcagca gggcgatccg gcgtcggtat cgttccggat tgccgcaccg
    18001 gcagcaccgt cgcggattga gctgacgccg ggctattttc agataaccgc aacgccgcat
    18061 cttgccgttt atgatccgac ggtacagttt gagttctggt tctcggaaaa gcggattacc
    18121 gatatcaggc aggttgaaac cacagcccgc taccttggca cggggctgta ctggatagcc
    18181 gccagtatca atatcaaacc gggccatgat tattactttt atatccgcag tgtgaacacc
    18241 gttggcaaat cggcattcgt ggaggccgtc ggtcgggcga gcgatgatgc ggaaggttac
    18301 ctggattttt tcaaaggcaa gataaccgaa tcccatctcg gtaaagagct gctggaaaaa
    18361 gttgatctga cggaggataa cgccagcaga ctggatgagt tttcgaaaga gtggaaggac
    18421 gctaacgata aatggaatgc catgtggggc gtcaaaattg agcagaccaa agacggcaaa
    18481 cattatgtcg cgggtattgg tctcagcatg gaggacacgg aggaaggcaa gctgagccag
    18541 tttctggttg ccgctaaccg tatcgcgttt attgacccgg caaacgggaa tgaaacgccg
    18601 atgtttgtgg cgcagggcaa ccagatattc atgaacgacg tgttcctgaa gcgcctgaca
    18661 gcccccacca ttaccagcgg tggcagccct ccggtatttt ccctgacatc agacggaaag
    18721 ctgaccgcta aaaatgcgga tatcagtggc agtgtgaatg cgaactcagg gacgctcaac
    18781 aacgtcacga ttaatgagaa ctgtcagatt aaggggaaac tgtcagccaa ccagattgaa
    18841 ggtgatattg tcaaaacggt cagcaagtct ttcccccgca cgagca
//
LOCUS       DAZFRN010000091        18521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFRN010000091 DAZFRN010000000
VERSION     DAZFRN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.091"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(18..641)
                     /gene="rsmG"
                     /locus_tag="ACHKUB_003096"
     CDS             complement(18..641)
                     /gene="rsmG"
                     /locus_tag="ACHKUB_003096"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO6656076.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(705..2594)
                     /gene="mnmG"
                     /locus_tag="ACHKUB_003097"
     CDS             complement(705..2594)
                     /gene="mnmG"
                     /locus_tag="ACHKUB_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO6656077.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2973..3416)
                     /gene="mioC"
                     /locus_tag="ACHKUB_003098"
     CDS             complement(2973..3416)
                     /gene="mioC"
                     /locus_tag="ACHKUB_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO6656078.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3506..3964)
                     /gene="asnC"
                     /locus_tag="ACHKUB_003099"
     CDS             complement(3506..3964)
                     /gene="asnC"
                     /locus_tag="ACHKUB_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO6656079.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4116..5108
                     /gene="asnA"
                     /locus_tag="ACHKUB_003100"
     CDS             4116..5108
                     /gene="asnA"
                     /locus_tag="ACHKUB_003100"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO6656080.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(5113..6564)
                     /gene="viaA"
                     /locus_tag="ACHKUB_003101"
     CDS             complement(5113..6564)
                     /gene="viaA"
                     /locus_tag="ACHKUB_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO6656081.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6558..8054)
                     /gene="ravA"
                     /locus_tag="ACHKUB_003102"
     CDS             complement(6558..8054)
                     /gene="ravA"
                     /locus_tag="ACHKUB_003102"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO6656082.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8277..10145
                     /gene="kup"
                     /locus_tag="ACHKUB_003103"
     CDS             8277..10145
                     /gene="kup"
                     /locus_tag="ACHKUB_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO6656083.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10312..10731
                     /gene="rbsD"
                     /locus_tag="ACHKUB_003104"
     CDS             10312..10731
                     /gene="rbsD"
                     /locus_tag="ACHKUB_003104"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO6656084.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10739..12244
                     /gene="rbsA"
                     /locus_tag="ACHKUB_003105"
     CDS             10739..12244
                     /gene="rbsA"
                     /locus_tag="ACHKUB_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO6656085.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12249..13214
                     /gene="rbsC"
                     /locus_tag="ACHKUB_003106"
     CDS             12249..13214
                     /gene="rbsC"
                     /locus_tag="ACHKUB_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO6656086.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13239..14129
                     /gene="rbsB"
                     /locus_tag="ACHKUB_003107"
     CDS             13239..14129
                     /gene="rbsB"
                     /locus_tag="ACHKUB_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO6656087.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14255..15184
                     /gene="rbsK"
                     /locus_tag="ACHKUB_003108"
     CDS             14255..15184
                     /gene="rbsK"
                     /locus_tag="ACHKUB_003108"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO6656088.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15188..16180
                     /gene="rbsR"
                     /locus_tag="ACHKUB_003109"
     CDS             15188..16180
                     /gene="rbsR"
                     /locus_tag="ACHKUB_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO6656089.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16146..17573)
                     /gene="mdtD"
                     /locus_tag="ACHKUB_003110"
     CDS             complement(16146..17573)
                     /gene="mdtD"
                     /locus_tag="ACHKUB_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO6656090.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17596..18288)
                     /gene="yieP"
                     /locus_tag="ACHKUB_003111"
     CDS             complement(17596..18288)
                     /gene="yieP"
                     /locus_tag="ACHKUB_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO6656091.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4459 a         4589 c         4839 g         4634 t
ORIGIN      
        1 gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg acgttcgcca
       61 tccagggctg gaacctgaag tttaaccact gattcgacct gatattcttc gggcaacaaa
      121 gcgatttcat cttccggcat ttgccctttt agcgcgtaga aacggccttg ctcaccagga
      181 agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct aattacgcca
      241 tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggttc aatattctcc
      301 agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag gctatccaac
      361 agagtgaaat gggcttcagg acgcacgata gagagtggaa tgccaggcag tccaggaccg
      421 gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat gctatcgaga
      481 atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta cgctttgttc
      541 catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc ggtaagcgaa
      601 atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac ctgttcttga
      661 tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc gctacgacgc
      721 agcataccct gttttttcag ccacaccagc agaatggaga tggccgcagg cgtgacgcca
      781 gaaatacgcg aagcctggcc gatagaggct ggtttgtgat cgttaagttt ggcgatcact
      841 tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag ggtgttctcg
      901 ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc gtatttaacc
      961 tgaatctcaa cctgttccgc cgcctgttcg tctgtcaatg caggggcaaa cggcgtcagc
     1021 gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc actggcttca
     1081 cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc cgacggggtt
     1141 acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt ctcgttaaag
     1201 cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt caaacgcaga
     1261 tccgcattat cttcgcgtag catcagacga tattctgcgc gcgaagtaaa catacgatac
     1321 ggttctttgg ttcctaaagt gcacaggtca tcaactagta cgccgagata cgcctgagaa
     1381 cgtgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag accggccagc
     1441 aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc agcaaagaac
     1501 agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgaggatc gaagaagtca
     1561 tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg catagagcgg
     1621 acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg ataaatttca
     1681 ttagaggtca gtccttccgg ttcaaggaag atctgatgct gatttctgtc ggcgaagcgc
     1741 atgactttgt cttcgatcga cgggcagtag cgtgggccga caccttcgat cacccctgca
     1801 tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc gttggtatga
     1861 gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa cgagaatacc
     1921 ggcattgggt tatcgccatg ctgttgcgcc agcacactaa agtcgatagt tcgagcatca
     1981 atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc acgcaaacgg
     2041 cgagaaagcg gaatggacgg cggatcacca gcacggccac cgctgtaatt atccagaccg
     2101 atatgaattt taccgtcgag gaacgtccca acggtgagca cgacggcttt ggcacggaac
     2161 ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat aagatcttca
     2221 accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg taccgcctga
     2281 cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc tttgcttgcg
     2341 tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag accgccgagt
     2401 gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg gttgcagctc
     2461 atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc catacgcgcc
     2521 gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac gtcaaaagga
     2581 tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat tgaagcccgg
     2641 gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg ggtattaaaa
     2701 agaagatcta tttatttaga gatctgttct attgtgatct cttattagga tcgcactgcc
     2761 ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc attaactgtg
     2821 aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta tacacaactc
     2881 aaaaactgaa caacggttgt tctttggata actaccggtt gatccaagct tcctgacaga
     2941 gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat ccacgatccc
     3001 agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt
     3061 tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc gatagcccca
     3121 caaaaggtgt catattcacg actgccaata ccgattgcgc caaagcggac cgcagaaaga
     3181 tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg aatatctccg
     3241 gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc ttccaacagc
     3301 ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc caggtgttct
     3361 gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc tgccataaac
     3421 cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat ctacctgtgg
     3481 aaaagtatgg gattaaaaaa gccgattatg gcttgatggt acgcatgatc gggttttgca
     3541 ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg ttgataagta
     3601 catgctggag agcgtcgatc gaacggcaca tcacttttat aaagatgctg tagtggccgg
     3661 ttgtgtaata ggcttcagtg acttcatcaa ggctttccag ctttgccagc gcggaagggt
     3721 agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa ccgagctgct
     3781 tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt ttctctactc
     3841 gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg taagcggtgc
     3901 gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc agataatttt
     3961 ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct tttttaatga
     4021 atcaaaagtg tgttaggctt tttattgaat gattattgca tgtgtgtcgg tttttgttgc
     4081 ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac attgccaaac
     4141 aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa cgtctggggc
     4201 tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag gataacttgt
     4261 cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc cagttcgaag
     4321 tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg
     4381 gcgaaggact gtacacgcac atgaaagccc ttcgccccga tgaagaccgt ctttctccgt
     4441 tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac ggtgagcgtc
     4501 aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa gcaaccgaag
     4561 ctgcggttag cgaagagttt ggcctggcac cgttcctgcc ggatcagatc cacttcgtac
     4621 acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt gagcgggcga
     4681 tagcgaaaga tcttggcgcg gtattccttg tcgggattgg cggcaagctg agcgatggtc
     4741 atcgccacga cgtgcgcgca ccggattatg atgactggag caccccgtca gagctgggtc
     4801 atgcgggtct gaacggcgat attctggtgt ggaaccctgt actggaagat gcgtttgagc
     4861 tttcttccat ggggatccgc gtggatgccg acacgttgaa gcatcagttg gcgctgaccg
     4921 gtgatgaaga tcgtctggag ctggagtggc atcaggcgct gctgcgcggt gaaatgccgc
     4981 agaccatcgg cggcggtatc ggccagtctc gtttgaccat gctgctgctg caactgccgc
     5041 atatcggcca ggttcagtgt ggtgtatggc cagctgctgt tcgcgagaac gtcccttctc
     5101 tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa
     5161 gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg acatcgccac
     5221 ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg tcacgtcgtc
     5281 aggcaaccgc tgagcgataa aatcagaaat caccaccgcg tcggcatcaa accattccct
     5341 gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc cgccacgaaa
     5401 ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa gctcataacg
     5461 gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga gagcaatgcg
     5521 catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca ttgagccgga
     5581 agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt tatgtaccac
     5641 cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg tgagcaactg
     5701 tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta gtgtcgccag
     5761 ttctggcggc aggagacgta aaatatcatc gctttgttgc agaccatcaa cctgctcagg
     5821 aacagttgcc ggttcgcgca ccatggtgcg gaaggcttcc atctgcgcat cgttacgcgg
     5881 tattgatttg gcttcccggg aacgccccag ctgttctgcc aggcgtttca gttccggctg
     5941 ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt taagctgacc
     6001 ggcgctcata tcccacagac gaccagctgc ggtattgtta tctgcgagaa tcggttcaag
     6061 ttgtccgctc agcgtcatgc gttcctgaac ttcacttaac agttgttcgc gttcttcttc
     6121 taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc aacgctggag
     6181 gaaaagcgta tgtaacgctg aagtgatcgt gctgttagca tcaaccaact gtcgggcttg
     6241 ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta gctgcacaat
     6301 aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt cggtgagttc
     6361 cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc ggggaacatc
     6421 atcagtgatt gccgccttca gtcgtgggaa tttttcaaag aagactgcca gctgcggtga
     6481 ggccagcagc gcgatgatca tctcttcgat caatccctct tcgctgacgg ccagcatcac
     6541 attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcgcc gacatcctgt
     6601 aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt ttgctgttcg
     6661 ctgaataaag cgtgttgctt gcgccagtca ctttcaagct cttccagttg ttgttttatt
     6721 tcacctggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc ttgtaaactt
     6781 acatcgcgta caacaagatg ttgggcgcta tccacttcca gattcaattt ctgggcaaag
     6841 ccgataccgt tcagtttgcc gcgaatttca ccacctttac tcagccactg ttccagcgcg
     6901 ttacgctcaa aggagatatg aaccacttcc atatcatgca attttaacgg tttttgcagc
     6961 agcagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg acgacggctg
     7021 aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg ctgctggagt
     7081 tgcaggtgac gttgcacaat cgcgccaagg cgagtcaaca tcccttgctg ttgccaggcg
     7141 tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac
     7201 cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg accgctaaaa
     7261 aaggcgctgg cctgcaataa gcggatcgct tttttccagc gacgatccga gacataaggc
     7321 gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa tacatgatcg
     7381 ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc tgtgacctgc
     7441 aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat ggagcggaaa
     7501 ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc atataacgct
     7561 tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag acgcatcggg
     7621 attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt gagcaaggta
     7681 ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc tgcttccggc
     7741 aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg
     7801 ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa cgcgcgggca
     7861 ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc tggcgggcca
     7921 aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat ggcgtggcta
     7981 cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc cgctaataaa
     8041 tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt
     8101 atagacgttt cttgctggtg gctaaaatag tctcaaaggg ggggtatttt tctttgagcc
     8161 aggttaatgt ggccgcattt aggagtacga ttttgccgtt aatcgtgcat actgtgcgct
     8221 tttttgtggg ccaagggact aagcacacat ttcatatttc aacgaaagac tagtctatga
     8281 gcactgataa taagcaatca ttgcccgcga ttaccctcgc ggcgattgga gttgtctacg
     8341 gcgatattgg taccagcccg ttatatacac ttcgtgaatg tttgtccggc cagtttggtt
     8401 ttggcgttga acgcgatgcc gtgtttggct ttttatcgct gatcttctgg ctgctaatct
     8461 ttgtggtttc cattaaatat ctcaccttcg tgatgcgggc agataacgcc ggtgaggggg
     8521 ggatcctgac gttgatgtcg cttgccgggc gtaatacgtc ggcgcgaacc acatcaatgc
     8581 tggtgattat ggggctaatc ggcggcagct ttttctatgg tgaagtcgtc ataacacccg
     8641 ctatttcggt gatgtcagcc attgaaggtc tggaaatcgt cgccccgcag ctggatacct
     8701 ggatagttcc cctctcaatt atcgttctca ctttattatt tatgattcaa aaacatggca
     8761 cggctatggt cggtaagctg tttgcgccga tcatgctgac ctggtttttg attctggcag
     8821 gactggggtt acgtagcatt attgctaacc cggaagtgct gcatgcgctg aatccaatgt
     8881 gggcggtgca tttcttcctt gaatacaaaa cggtttcttt tattgcatta ggggcagtgg
     8941 tgttgtcgat tacgggggtc gaggcgctgt atgctgatat ggggcacttt ggtaagttcc
     9001 ctattcgcct ggcgtggttc accgtcgtat taccttcctt gacccttaat tacttcggtc
     9061 agggagcgct gttgttaaag aacccggaag cgattaagaa cccgttcttc ctgttggcac
     9121 cggactgggc gctgatcccg ctgctgatca tcgccgcact ggcgacggta attgcctcgc
     9181 aggcggttat ctctggcgtc ttctcattga cgcgtcaggc ggtacgtctg ggatatttgt
     9241 cgccgatgcg cattattcac acctccgaaa tggagtcagg gcaaatctat attccgtttg
     9301 tgaactggat gctctatgtc gcggtcgtga ttgtgattgt cagctttgag cactccagca
     9361 acctggcggc ggcgtacggg attgcggtga ccggaaccat ggtgctgacg tctattctct
     9421 cgactaccgt ggcacgtcag aactggcact ggaataagta ttttgttgcg ctgatcctga
     9481 ttgctttcct ttgtgtcgat attccattgt tcaccgctaa cctcgataaa ctgctctccg
     9541 gcggctggtt gccattgagc ctcggtacgg tgatgtttat cgtgatgacc acctggaaga
     9601 gcgagcgttt ccgcttgctg cggcggatgc atgaacatgg taactctctg gaagcgatga
     9661 ttgcttcgct ggagaaatca ccgcctgttc gcgtgcccgg gaccgcggtg tatatgtcgc
     9721 gtgcaatcaa cgtcattccc tttgcgctga tgcataacct taaacataac aaggtattgc
     9781 atgagcgggt gattctgtta actctgcgta ccgaagacgc gccatatgtc cataatgtcc
     9841 gtcgggtaca gattgaacaa ctgtcgccca ctttctggcg cgtggtggca agttatggtt
     9901 ggcgagaaac gccaaacgtg gaagaagttt tccaccgctg cggtctggaa ggattaagtt
     9961 gccggatgat ggaaacctcc ttctttatgt cgcatgagtc gttgatcctc ggcaaacgcc
    10021 cgtggtattt gcgtctgcgc ggcaagctgt acttgctgct gcaacgtaat gcgctgcgtg
    10081 caccagatca atttgaaatt ccgccaaaca gggttatcga actgggtact caggtcgaaa
    10141 tctaacgcca gacgcctcct ttctccataa gggggcgttt ttgttttcat ggttaatcac
    10201 catgtaaaac gtttcgaggt tgatcacatt tccgtaacgt cacgatggtt ttcccaactc
    10261 agtcaggatt aaactgcggg tcagcgaaac gtttcgctga tggagaaaaa aatgaaaaaa
    10321 ggcaccgttc ttaattctga tatttcatcg gtgatctccc gtctgggaca taccgatacg
    10381 ctggtggtgt gtgatgctgg tttacccatc cccaaaagta caacgcgtat cgatatggca
    10441 ttaacccagg gtgtaccttc ttttatgcag gtgctgggcg tcgtcacaaa tgaaatgcag
    10501 gtcgaggctg ccattatcgc ggaagagatc aaacaacata atccgcaact ccacgaaacg
    10561 ttgctcactc accttgagca gctgcaaaaa caccagggaa ataccattga aattcgttac
    10621 accacgcatg aacaattcaa acaacaaacc gcagaaagtc aggcggtaat tcgcagcgga
    10681 gaatgttctc cgtatgcgaa tatcattctc tgtgctggcg tgacgttctg aggccgtcat
    10741 ggaagcatta cttcagctta aaggcatcga taaagccttc ccgggcgtaa aagccctctc
    10801 gggcgcagcg ttaaatgtct atccgggccg cgtgatggcg ctggtgggcg aaaacggcgc
    10861 gggtaaatcc accatgatga aagtgcttac tggtatctat gctcgcgatg ccggcacgct
    10921 tttatggctg gggaaagaaa cgacatttac cgggccgaaa tcttcccagg aagccgggat
    10981 tgggattatc catcaggaac tgaacctgat cccgcagttg accattgccg aaaacatttt
    11041 cctcggtcgt gagtttgtta atcgctttgg caaaattgac tggaaaacca tgtatgccga
    11101 agcggataaa ttgctggcta aacttaacct gcgctttaaa agcgacaagc tggtgggcga
    11161 tctttccatc ggtgaccagc aaatggttga aatcgccaaa gtgctgagct ttgagtcgaa
    11221 agtcatcatt atggatgaac cgaccgatgc gctgaccgat accgaaaccg aatccctgtt
    11281 ccgcgtcata cgcgagctga aatcgcaagg ccgcggtatt gtctatatct cccaccgcat
    11341 gaaagaaatc ttcgagattt gcgatgacgt taccgttttt cgtgacgggc aatttattgc
    11401 tgagcgcgaa gtggcatcac tgaccgaaga ttcgctgatt gagatgatgg tgggccgcaa
    11461 gctagaagat caatatccac acctggacaa agcgccggga gatatccgcc tgaaagtcga
    11521 taatctctgc ggacctggcg ttaacgatgt ctcttttact ttacgcaaag gcgaaattct
    11581 tggcgtctct ggtttgatgg gcgcaggtcg taccgaactg atgaaagtgc tctacggcgc
    11641 actaccgcgc accagcggtt acgtcaccct ggatgggcat gaagtcgtta cccgttcacc
    11701 gcaggatggc ctggcaagcg gcattgtgta tatctccgaa gaccgtaaac gtgacggttt
    11761 agtgttgggc atgtcagtaa aagagaacat gtcgctgaca gcgctgcgct acttcagccg
    11821 cgctggcggc agtttgaagc atgccgatga acagcaggct gtgagtgatt tcattcgtct
    11881 gtttaatgtg aaaacaccgt cgatggaaca ggcaattggt ctgctttccg gtggcaatca
    11941 gcaaaaagtg gcgattgccc gcggtctgat gacacgcccc aaagtgttga tccttgatga
    12001 acctacccgt ggcgtagatg tcggcgcgaa aaaagagatc tatcaactga ttaaccagtt
    12061 caaagccgat ggcttgagca tcattctggt gtcatcggag atgccagaag tattaggcat
    12121 gagcgatcgc atcatcgtca tgcatgaagg gcatctcagc ggggaattta ctcgtgagca
    12181 ggccacccag gaagtgttaa tggctgccgc tgtgggcaag cttaatcgcg tgaatcagga
    12241 gtaaaaaaat gacaacccag actgtctctg gtcgccgtta tttcacgaaa gcgtggctga
    12301 tggagcagaa atcgcttatc gctctgctgg tgctgatcgc gattgtctcg acgttaagcc
    12361 cgaacttttt caccatcaat aacttattca atattctcca gcaaacctcg gtgaacgcca
    12421 ttatggcggt cgggatgacg ctggtgatcc tgacgtcggg catcgacttg tctgtaggtt
    12481 ctctgttggc gctaaccggc gcagttgctg catctatcgt cggcattgaa gtcaatgcgc
    12541 tggtggctgt cgctgccgct ctcgcgttag gtgccgcaat tggtgcggta accggggtga
    12601 ttgtagcgaa aggtcgcgtt caggcgttta tcgctacgct ggttatgatg cttttactgc
    12661 gcggcgtgac catggtttat accaacggta gcccagtgaa taccggcttt actgagaacg
    12721 ccgatctgtt tggctggttt ggtattggtc gtccgctggg cgtaccgacg ccagtctgga
    12781 tcatggggat tgtcttcctc gcggcctggt acatgctgca tcacacgcgt ctggggcgtt
    12841 acatctacgc gctgggcggc aacgaagcgg caacgcgtct ttctggtatc aacgtcaata
    12901 aaatcaaaat catcgtctat tctctttgtg gtctgctggc atcgctggcc gggatcattg
    12961 aagtggcgcg tctctcctcc gcacaaccca cggcggggac tggctatgag ctggatgcta
    13021 ttgctgcggt ggttctgggc ggtacgagtc tggcgggcgg aaaaggtcgc attgttggga
    13081 cgttgatcgg cgcattaatt cttggcttcc ttaataatgg attgaatttg ttaggtgttt
    13141 cctcctatta ccagatgatc gtcaaagcgg tggtgatttt gctggcggtg ctggtagaca
    13201 acaaaaagca gtaataacga ctacaggaca tcttgaatat gaacatgaaa aaactggcta
    13261 ccctggtttc cgctgttgcg ctaagcgcca ccgtcagtgc gaatgcgatg gcaaaagaca
    13321 ccatcgcgct ggtggtctcc acgcttaaca acccgttctt tgtatcgctg aaagatggcg
    13381 cgcagaaaga ggcggataag cttggctata acctggtggt gctggactcc cagaacaacc
    13441 cggcgaaaga gctggcgaac gtgcaggact taaccgttcg cggcaccaaa attctgctga
    13501 ttaacccgac cgactccgac gcagtaggta atgctgtgaa gatggctaac caggcgaaca
    13561 tcccggttat cactcttgac cgccaggcaa cgaaaggtga agtggtgagc cacattgctt
    13621 ctgataacgt actgggtggc aaaatcgccg gtgattacat cgcgaagaaa gcgggtgaag
    13681 gtgccaaagt tatcgagctg caaggcattg ctggtacatc cgcagcccgt gaacgtggcg
    13741 aaggcttcca gcaggccgtt gctgctcaca agtttaatgt tcttgccagc cagccagcag
    13801 attttgatcg cactaaaggt ttgaacgtaa tgcagaacct gttgaccgct catccggatg
    13861 ttcaggctgt attcgcgcag aatgatgaaa tggcgctggg cgcgctgcgc gcactgcaaa
    13921 ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg tacaccggat ggcgaaaaag
    13981 cggtgaatga tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga
    14041 aaggcgtcga aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg
    14101 ttgatctgaa actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac
    14161 ataaatacgt catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc
    14221 taacacggtg gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt
    14281 tcttggcagc attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga
    14341 aaccgtaacc ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt
    14401 ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc tgtacgggtg atgacagcat
    14461 tggtgagagc gttcgccagc agctcgccac tgataacatt gatattactc cggtcagcgt
    14521 gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt
    14581 catcggtatt catgccggcg ctaatgctgc cctttccccg gcgctggtgg aagcgcaacg
    14641 tgagcgtatt gccaacgcgt cagcattatt aatgcagctg gaatcaccac tcgaaagtgt
    14701 gatggcagcg gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc
    14761 ggctcgcgaa cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac
    14821 ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc
    14881 gcaggtactg catgaaaaag gtatccgtac tgtactgatt actttaggaa gtcgtggtgt
    14941 atgggctagc gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga
    15001 taccattgct gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa
    15061 accattgcca gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa
    15121 aggcgcacaa ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag
    15181 gtgacgcttg gctacaatga aagatgttgc ccgcctggcg ggcgtttcta cctcaacagt
    15241 ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc
    15301 ggcgattaaa gaactcaatt acgcgccatc agccctggcg cgtagcctca aactcaatca
    15361 aacacatacc attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt
    15421 gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga
    15481 aggcgatgaa cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg
    15541 cttgctgtta ctgtgcaccg aaacgcatca accttcgcgt gaaatcatgc aacgttatcc
    15601 gacagtgcct actgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca
    15661 ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac
    15721 ccgtatcgcc tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg
    15781 ttatcgggcg gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg
    15841 tgattttgaa tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg
    15901 tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata
    15961 tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact
    16021 ggcaagcttt atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga
    16081 gctggcgatt gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt
    16141 acaacttact ccgattctga tggaacgcgg ttcggcttag atttacgctg tcttttgatc
    16201 aaattattac catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata
    16261 atgcccatcg tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct
    16321 tcataaacgc gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc
    16381 gtgaccgcca gaacactgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg
    16441 atggtattca tcgcggtaaa ttgcgtcgac atagccatcc ctaatataaa caacggcaag
    16501 atcagcatcc atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca
    16561 ataatcaccg tgatccccac taacgtatgg cgatagccca gacgacgtaa gacttgggta
    16621 accatcgatt ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata
    16681 aacgcctgat aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacaaccg
    16741 gtccccagac gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat
    16801 aaatctaatg aaattaatgg gtttggcgtg tgtcgcgcat ggagaatata gagaagcagt
    16861 aacccgatgc tggtgacaat taccgtcaag gcaatccagc tggcgacaat cctttccccg
    16921 aatagctcta ttccgcttga gaagagaaca aggctgaggc caaacagcaa aaagccagtg
    16981 atatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg
    17041 cccgcaatac ctatggggat attgattaaa aatatccagt gccaggttgc ccaggtgacc
    17101 agcacgccgc caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa
    17161 ttcaatactg gaagaagttc attacgagga taagcgcgca gtaaggccag ccgagcaaca
    17221 ggcatcatca ttgcgccgcc tatcccctga ataacccgga agacaaccag ctgtggtagc
    17281 gaattagaaa gtgcgcaggc cagagaaccc aatgtgaaca gactcacggc aagggtaaaa
    17341 atgcgacgcg taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc
    17401 gtcagcgtat aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga
    17461 gcgattgcgg gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaagaaggcc
    17521 atcgccgcga tccacggcaa acccgccata ctgcgcttct ttttatcgct cattcaatgt
    17581 cctgttatcg ggttgtcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc
    17641 cgcgtcgcca tcgctttgga caatcgcatc gacaatcgcc tggtgcagat ccagctttat
    17701 cactgtgtcg ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc
    17761 gaatgaggtc aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggccatatc
    17821 gacttcgatc cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa
    17881 cgtattgaga tgcgccttct gttccgcggt gccaaccgtt gcagccagta ggcaagcttg
    17941 cggctccaga cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc
    18001 tgtcatccac caggtaagca attcctgatc aagaaaattc cagtttgatt gtggcatgac
    18061 ccgggtacca attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc
    18121 ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccaggcaa
    18181 aatggtgccg ggttcatatt caccttttaa gatgcgttgc gccagcttct cagccagaac
    18241 atacgaaagg tttttctgtg cagctaactg ttgtgcgctt aaaggcatta ctaatcttcc
    18301 tttttctttt tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg
    18361 gcaaattgct tgttttatgg cacattaacg gggcttttgc tgaaaaaatg cgcggtcaga
    18421 aaattatttt aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg
    18481 acacggaaca acggcaaaca cgccgccggg tcagcggggt t
//
LOCUS       DAZFRN010000092        18220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFRN010000092 DAZFRN010000000
VERSION     DAZFRN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.092"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            10..84
                     /gene="tnaC"
                     /locus_tag="ACHKUB_003112"
     CDS             10..84
                     /gene="tnaC"
                     /locus_tag="ACHKUB_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO6656092.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            305..1720
                     /gene="tnaA"
                     /locus_tag="ACHKUB_003113"
     CDS             305..1720
                     /gene="tnaA"
                     /locus_tag="ACHKUB_003113"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO6656093.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1811..3058
                     /gene="tnaB"
                     /locus_tag="ACHKUB_003114"
     CDS             1811..3058
                     /gene="tnaB"
                     /locus_tag="ACHKUB_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO6656094.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWIGNVFNVLPKFG"
     gene            3191..4366
                     /gene="mdtL"
                     /locus_tag="ACHKUB_003115"
     CDS             3191..4366
                     /gene="mdtL"
                     /locus_tag="ACHKUB_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO6656095.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4341..5300
                     /gene="yidZ"
                     /locus_tag="ACHKUB_003116"
     CDS             4341..5300
                     /gene="yidZ"
                     /locus_tag="ACHKUB_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO6656096.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5457..6206
                     /gene="yieE"
                     /locus_tag="ACHKUB_003117"
     CDS             5457..6206
                     /gene="yieE"
                     /locus_tag="ACHKUB_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO6656097.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6228..6794
                     /gene="yieF"
                     /locus_tag="ACHKUB_003118"
                     /gene_synonym="chrR"
     CDS             6228..6794
                     /gene="yieF"
                     /locus_tag="ACHKUB_003118"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO6656098.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6848..8185)
                     /gene="adeP"
                     /locus_tag="ACHKUB_003119"
     CDS             complement(6848..8185)
                     /gene="adeP"
                     /locus_tag="ACHKUB_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO6656099.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            8352..9017
                     /gene="yieH"
                     /locus_tag="ACHKUB_003120"
     CDS             8352..9017
                     /gene="yieH"
                     /locus_tag="ACHKUB_003120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO6656100.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9084..9557
                     /gene="cbrB"
                     /locus_tag="ACHKUB_003121"
     CDS             9084..9557
                     /gene="cbrB"
                     /locus_tag="ACHKUB_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO6656101.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9606..10193
                     /gene="cbrC"
                     /locus_tag="ACHKUB_003122"
     CDS             9606..10193
                     /gene="cbrC"
                     /locus_tag="ACHKUB_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO6656102.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10255..10977)
                     /gene="yieK"
                     /locus_tag="ACHKUB_003123"
     CDS             complement(10255..10977)
                     /gene="yieK"
                     /locus_tag="ACHKUB_003123"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6656103.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVKFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10992..12161)
                     /gene="yieL"
                     /locus_tag="ACHKUB_003124"
     CDS             complement(10992..12161)
                     /gene="yieL"
                     /locus_tag="ACHKUB_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6656104.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12188..13804)
                     /gene="bglH"
                     /locus_tag="ACHKUB_003125"
     CDS             complement(12188..13804)
                     /gene="bglH"
                     /locus_tag="ACHKUB_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO6656105.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNEQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENYMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13890..15284)
                     /gene="bglB"
                     /locus_tag="ACHKUB_003126"
     CDS             complement(13890..15284)
                     /gene="bglB"
                     /locus_tag="ACHKUB_003126"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO6656106.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15303..17180)
                     /gene="bglF"
                     /locus_tag="ACHKUB_003127"
     CDS             complement(15303..17180)
                     /gene="bglF"
                     /locus_tag="ACHKUB_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO6656107.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17312..18148)
                     /gene="bglG"
                     /locus_tag="ACHKUB_003128"
     CDS             complement(17312..18148)
                     /gene="bglG"
                     /locus_tag="ACHKUB_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO6656108.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4603 a         4533 c         4299 g         4785 t
ORIGIN      
        1 aagtgcatta tgaatatctt acatatatgt gtgacctcaa aatggttcaa tatcgacaat
       61 aagattgtcg atcaccgccc ttaatttacc cttctgtagc catcaccaga gccaaaccga
      121 ttagattcaa tgtgaacgat tagtttgcta tatcttaatt ttgccttttg caaaggccat
      181 ctctcgttta tttacttatt ttagtaaatg atggtgcttg tatatataca tggcgaatta
      241 atggatattg cagatgtaat attcacaggg atcactgtaa ttaaaataaa tgaaggatta
      301 tgtaatggaa aactttaaac atctccctga accgttccgc attcgtgtta ttgagccagt
      361 aaaacgtact actcgcgctt atcgtgaaga agcaattatt aaatccggta tgaacccgtt
      421 cctgctggat agcgaagatg tgtttatcga tttactgacc gacagcggca ccggggcggt
      481 aacccaaagt atgcaggcag cgatgatgcg cggcgacgaa gcctacagcg gcagccgcag
      541 ctactatgcg ttagccgagt cagtgaaaaa tatctttggt tatcaataca ccattccgac
      601 tcaccagggc cgtggcgcag agcaaattta tattccggta ctgattaaaa aacgcgagca
      661 ggaaaaaggc ctggatcgca gcaaaatggt ggcattctct aactatttct ttgataccac
      721 gcagggccat agccagatta acggctgtac cgtgcgtaac gtctatatca aagaagcctt
      781 cgatactggc gtgcgttacg actttaaagg taactttgac cttgagggat tagaacgcgg
      841 tattgaagaa gttggcccga ataacgtgcc gtatatcgtt gcaaccatca ccagtaactc
      901 tgcaggtggt cagccggttt cactggcaaa cttaaaagcg atgtacagca tcgcgaagaa
      961 atacgatatt ccggtggtaa tggactccgc acgctttgct gaaaacgcct atttcatcaa
     1021 gcagcgtgaa gcagaataca aagactggac catcgagcag atcacccgcg aaacctacaa
     1081 atatgccgat atgctggcga tgtccgccaa gaaagatgcg atggtaccga tgggcggctt
     1141 gctgtgcatg aaagacgaca gcttctttga tgtgtacacc gagtgcagaa ccctttgcgt
     1201 ggtgcaggaa gggttcccga catatggcgg cctggaaggc ggcgcgatgg agcgtctggc
     1261 ggtaggtctg tatgacggca tgaatctcga ctggctggct tatcgtatcg cgcaggtaca
     1321 gtatctggtc gatggtctgg aagagattgg cgttgtctgc cagcaggcgg gcggtcacgc
     1381 ggcattcgtt gatgccggta aactgctgcc gcatatcccg gcagaccagt tcccggcaca
     1441 ggcgctggcg tgcgagctgt ataaagtcgc cggtatccgt gcggtagaaa ttggctcttt
     1501 cctgttaggc cgcgatccga aaaccggtaa acaactgcca tgcccggctg aactgctgcg
     1561 tttaaccatt ccgcgcgcaa catatactca aacacatatg gacttcatta ttgaagcctt
     1621 taaacatgtg aaagagaacg cggcgaatat taaaggatta acctttacct acgaaccaaa
     1681 agtattgcgt cacttcaccg caaaactgaa agaagtttaa ttaatactac agagtggcta
     1741 taaggatgtt agccactctc ttaccctaca tcctcaataa caaaaatagc cttcctctaa
     1801 aggtggcatc atgactgatc aagctgaaaa aaagcactct gcattttggg gtgttatggt
     1861 tatagcaggt acagtaattg gtggaggtat gtttgcttta cctgttgatc ttgccggtgc
     1921 ctggtttttc tggggtgcct ttatccttat cattgcctgg ttttcaatgc ttcattccgg
     1981 gttattgtta ttagaagcaa atttaaatta tcctgtcggc tccagtttta acaccatcac
     2041 caaagattta atcggtaaca cctggaacat tatcagcggt attaccgttg ccttcgttct
     2101 ctatatcctc acttatgcct atatctctgc taatggtgcg atcattagtg aaacgatatc
     2161 aatgaatttg ggctatcacg ctaatccacg tattgtcggg atctgcacag ccattttcgt
     2221 tgccagcgta ttgtggataa gttcgttagc cgccagtcgc attacctcat tgttcctcgg
     2281 gctgaagatt atctcctttg tgatcgtgtt tggttctttc ttcttccagg tcgattactc
     2341 catcctgcgc gatgccacca gcaccactgc gggaacgtct tacttcccgt atatctttat
     2401 ggctttgccg gtgtgtctgg cgtcatttgg tttccacggc aatattccca gcctgattat
     2461 ttgctatgga aaacgcaaag ataagttaat caaaagcgtg gtatttggtt cgctgctggc
     2521 gctggtgatt tatctcttct ggctctattg cacgatgggg aatattccgc gcgaaagctt
     2581 taaggcgata atctcctcag gcggcaacgt tgattcgctg gtgaaatcgt tcctcggcac
     2641 caaacagcac ggcattatcg agttttgcct gctggtgttc tctaacttag ctgttgctag
     2701 ttcgttcttt ggtgtcacgc tggggttgtt cgattatctg gcggacctgt ttaagattga
     2761 taactcccac ggcgggcgtt tcaaaaccgt gctgttaacc ttcctgccac ctgcgttgtt
     2821 gtatctgatc ttcccgaacg gctttattta cgggatcggc ggtgccgggc tgtgcgccac
     2881 catctgggcg gtcattattc ccgcagtgct tgcaatcaaa gctcgcaaga agtttcccaa
     2941 tcagatgttc acggtctggg gcggcaatct tattccggcg attgtcattc tctttggcat
     3001 aaccgtaatt ttatgctgga tcggtaacgt ctttaacgtg ttacctaaat ttggctgatc
     3061 ccttcaagaa gccagccatt tcgctggctt ctttcctgtc aggaaatctc ttttgtccaa
     3121 atggcaactc gcctgattct ccttcaccac gtatgctttg cgtcacctta ctatcaggac
     3181 gctttagccc atgtcccgct ttttaatttg tagttttgcc ctggttttac tttatcccgc
     3241 cgggattgat atgtacctcg ttggtttacc gcgcatcgcc gccgatctca atgccagcga
     3301 agcgcagttg catattgcgt tctccgtata tctggcgggg atggcagctg cgatgttatt
     3361 tgccggtaaa gtggccgatc gttcagggag aaagccggtc gccatacccg gcgcggcgct
     3421 atttattatt gcctcggtgt tctgttcact ggctgaaacc agcacgttat ttcttgcagg
     3481 ccgatttcta caggggttgg gcgcaggctg ttgttacgta gtggcgttcg ccattttgcg
     3541 cgacacgctg gatgatcgac gtcgggctaa agtgctgtca ttactcaacg gtattacctg
     3601 catcattccg gtgttagcgc cagtgctcgg acatctgatt atgcttaaat tcccgtggca
     3661 gagtctgttc tgggcgatgg caatgatggg catcgcggta ctgatgttgt ctttgtttat
     3721 tttaaaagaa acgcgcccag cgtcccccgc tgcttcggac aaaccacgag aaaatagcga
     3781 gtcgctgctt aatcggtttt tcctcagccg tgttgttatc accaccctca gcgtttcggt
     3841 gatcctcact ttcgtcaata catcgccggt attgctgatg gaaatcatgg gttttgagcg
     3901 cggagaatac gccaccatta tggcgttgac tgctggcgtc agcatgaccg tttcattctc
     3961 cacgccattt gcgctgggaa tttttaagcc acgtacgttg atgatcacct cgcaggtgtt
     4021 attccttgca gcggggatca cccttgccgt ttcaccttcc catgcggttt ctctgtttgg
     4081 tatcacgctg atttgcgccg gtttctcggt aggttttggc gtagcgatga gtcaggcgtt
     4141 aggaccattt tcattacgcg cgggcgtagc cagctcgacc ttaggtattg cgcaggtttg
     4201 cggttcgtca ctgtggattt ggctggcagc ggtggttggt atcggcgcat ggaatatgct
     4261 gatcgggatt ctgattgcct gtagcatagt gagcctgttg ctgattatgt tcgtcgcgcc
     4321 tggacgcccc gttgccgctc atgaagaaat ccatcaccac gcttgatctc aatctgctgc
     4381 tctgtctgca actgctgatg caggagcgca gcgtgaccaa agcggcgaag cggatgaacg
     4441 tgaccccttc agcggtgagt aagtcgctgg caaagttaag agcgtggttt gacgacccgc
     4501 tctttgtgaa ctcaccgctg ggcctgtcgc ccacaccgct aatggtcagc atggagcaaa
     4561 atctggcgga atggatgcaa atgagcaatc tgctgctgga taaaccgcac caccagacac
     4621 cgcgcggcct gaagtttgag ttggcggcgg aatcgccgct gatgatgatc atgcttaatg
     4681 cgctgtcgaa acggatctac caacgttacc cgcaggcgac catcaaacta cgtaactggg
     4741 attacgattc cttagatgcc attactcgtg gtgaagtgga tattggtttt tccggtcgcg
     4801 aaagccatcc tcgctcgcgg gagctgttaa gctcgctacc gttagccatt gattatgaag
     4861 tgctgtttag tgatgtaccc tgcgtctggt tacgcaaaga tcatccggca ctgcatgaaa
     4921 cgtggaatct ggacaccttt ttacgttatc cgcatatcag catttgctgg gaacagagcg
     4981 acacctgggc gctggacaat gtgttacagg agctggggcg cgaacgcacg attgctatga
     5041 gcctgccgga attcgagcag tcactgttta tggcagcgca acccgacaat ctgctactgg
     5101 cgaccgcgcc gcgctactgt cagtactaca atcaactcca tcaactgccg ttggttgctc
     5161 ttcctctccc gtttgacgaa agccagcaaa aaaagctgga agttcctttt accctgctgt
     5221 ggcataaacg gaacagccat aatccgaaga tcgtctggtt acgggaaacc attaaaaacc
     5281 tttacgcgtc gatggcataa ccgaatcgta tgaaacgcga cccaatttca caataaaatg
     5341 taaaaaagtt gtaataagct tgtctgaatc gaacttttag ccgctttagt ctgtccatca
     5401 ttccagtaaa tgattactct tgtattcata atggaccatt aagcatggag cgaaaaatgg
     5461 cgactcactt tgcccgaggg attttaacgg aaggacatct gatttctgtt cgtctcccct
     5521 cccagtgtca tcaagaagcc cgaaacattc cccctcatcg tcaaagccgt tttctggcgt
     5581 ccagaggttt actcgcagaa ctgatgttca tgctgtatgg cattggcgaa ttgccggaaa
     5641 tcgtcaccct gccgaaaggt aaaccggttt tcagtgataa aaatttgcct tcgttttcta
     5701 tttcctatgc cgggaatatg gttggcgtgg cgttaacaac cgaaggtgaa tgtggcctcg
     5761 atatggaact acagcgtgcg acgcgcgggt ttcatagccc acacgcgccc gataaccaca
     5821 ccttttccag caatgaatcg ctatggatca gtaaacaaaa cgatcctaac gaagcgcggg
     5881 cgcagctcat cacgctgcgc cgaagcgtgc taaaactaac cggtgatgtt ttgaatgacg
     5941 atccgcgcga tctgcagctg ctgcccattg ccggacgcct gaaatgtgct catgtaaatc
     6001 atgtagaagc gttatgcgac gcggaggacg tgctagtgtg gtccgtggcg gtcacgcccg
     6061 cgattgaaaa gctcagtgtc tgggagttag atggcaaaca cagctggaaa agcctgccag
     6121 atattcacag ccgcgccaac aatcctacca gccggatgat gcgttttgcc cagctctcta
     6181 ccgtgaagtc tttttcgcca aattgataga caaccacagg agtcattatg tctgaaaaat
     6241 tgcaggtggt tacgttactg gggagcctgc gcaaaggctc atttaatggc atggttgcac
     6301 gtaccctgcc gaaaattgct ccggcaagca tggaagtcaa tgcgttacct tccattgccg
     6361 acattcctct gtatgacgct gacgtacagc aggaagacgg ttttccagca acggttgaag
     6421 ctctggcgga acagatccgt caggctgacg gtgtggtgat cgtcacgccg gaatataact
     6481 actcggtacc gggtgggctg aaaaatgcca tcgactggct ttcccgcctg ccggatcaac
     6541 cgctggcagg taaaccggtg ttgattcaga ccagctcaat gggcgtgatt ggcggcgcgc
     6601 gctgtcagta tcacctgcgc cagattctgg ttttcctcga tgcaatggtg atgaacaagc
     6661 cggaatttat gggcggcgtg attcagaaca aagttgatcc gcaaaccgga gaagtgattg
     6721 atcagggtac gctggaccac ctgaccgggc aattgaccgc atttggtgag tttattcagc
     6781 gagttaagat ctaaataaaa aacccgccag taatcatgca tggcgggttt ttaatgcgct
     6841 atcgatttta atgagcgtcg ataaatacaa tcttcaggat aaacatcagc gcaacgatga
     6901 ttacgcacgg gctaaggtca cgcagacgcc cggtaccaat cttcatcacg cagtaggaga
     6961 taaagcccag cgcaatacct tcggtaatcg aaaagctgaa cggcatcatc acggcggtaa
     7021 taaacgccgg aacagattca gtaagatcct gccagttcac gcgagcaaga cttgaggtca
     7081 tcaacacgcc aacgtagatc aacgcgccag ctgcagcgta gcctggcacc attcccgcca
     7141 gcggcgacag aaagataacc agcaggaaca gcagaccaac aaccactgcc gtcagaccgg
     7201 tacgaccgcc aaccgatacg ccggaagagg actcaatata agccgtaacg gaagaagtac
     7261 cgataaacga accggtcacg gaagagatac tgtcgacata cagcgcctgc ttcatgcgcg
     7321 ggaatttacc cttctcatcc gccaggcctg ctttatcggt cacgccaatc agcgtaccgg
     7381 aggagtcaaa caggttgacc agcatgaaag agaaaatcac ccctgccagc ccgaggttaa
     7441 acgacccggc taaatctaca tggcccacga cagtcattac gctcggcggc gcagaaacga
     7501 tgccattgta gtggacatca cccagcatcc agcccagcag cgttgtcacc acgatggaaa
     7561 ccagcaccgc cgcgtgaatg ttgcgcgagg ccagaatagc gatgatgaag aagccgagga
     7621 tccccagcag cacgctgtga gaagtcaggt taccgatgct gaccagcgtt tccgggttag
     7681 cgacaatcac acctgcattc ttcagtccca tcataccaat gaacagaccg ataccgctgg
     7741 tgatgcccac acgcagactt accggaatgt tagcaatcat ccagtaacga acgcggaaaa
     7801 tcgtcagtaa cagcagacca atcgcgcccc agaagattgc gcccatcccg acttgccacg
     7861 gcaagcccat agcctgtaca acgacaaaag cgaagaatgc attcaggccc atagcgggtg
     7921 ccagtgcaac tggcaggtta gcaaacagtc ccatcataat actgccgaat gcagcaatca
     7981 gacaggtagt aacgaagacg gcgctggtat ccatgccagc aacgccaaga atttgcgggt
     8041 taacaaaaac gatgtaaacc atcgtcagga aggtggtaaa accggcgatc acttcggtcc
     8101 gtgccgtcgt gccatgttcg cgcagtttaa acacgcgttc cagcatcccc tggccagaag
     8161 cctgggttgt gtgttgttga ctcattatct atttccgaac aaggagggaa aatccgtcgc
     8221 tatcgtatac caaaatgaga caatatggac ggctgaagat aacttttttt cattgtattt
     8281 gcttatacgg caacgattgc gtcatgcgaa ttggcatgac gaaaacgtta aactaacgat
     8341 aaaggaaaga catgtcccag atagaagcgg tatttttcga ctgcgacggt acgctggtcg
     8401 acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt ggtattacgc
     8461 tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa attatcgata
     8521 ttgtttccct tgaacatggt gttacgttag cgaaaacaga agctgaacac gtttaccgtg
     8581 cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct ggagcgctcc
     8641 tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc
     8701 attctatggg caagctgaat atgttgcact acttcccgga taaactgttc agcggctacg
     8761 atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg
     8821 taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa tctggtatcg
     8881 acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg atcgttcacc
     8941 cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa gcgcgtggtt
     9001 gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa ttggcgctca
     9061 aaggtaagtt aagggtgttt gatatgtctg tttcacgtcg ggtaatacat cacggacttt
     9121 attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac atattcttcg
     9181 caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt ctcttattgt
     9241 cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg
     9301 aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt ggcgtcgtta
     9361 tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc tcggagttgt
     9421 ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca ttgctggcag
     9481 gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca tgtcctgaaa
     9541 cagactcttt aagttaagcg ggatacttta tctttgggct actcaaaagc agacaggatg
     9601 ttactatgac tcaaaatatc aggccgttac cccaattcaa atatcatccc aagccactgg
     9661 aaacaggcgc atttgaacag gataaaaccg tagagtgcga ttgctgtgaa caacagacgt
     9721 cagtttatta ctcgggtccc ttttattgcg ttgatgaagt tgaacatctc tgtccgtggt
     9781 gtattgcgga cggttctgct gctgaaaagt ttgcaggtag ttttcaggat gatgccagca
     9841 tagaaggtgt tgaatttgag tatgatgaag aggacgaatt tgccggtatt aagaacacat
     9901 atcctgatga aatgctgaaa gagttggttg aacgcacgcc aggttatcat ggatggcaac
     9961 aggaattctg gcttgcgcat tgtggcgatt tctgtgcttt tatcggctat gtgggctgga
    10021 atgatataaa agatcgcctc gatgaatttg ccaaccttga agaagattgt gagaatttcg
    10081 gtattagaaa ttctgatcta gctaaatgcc tgcaaaaggg tggtgattgt cagggttatc
    10141 tcttccgctg tctccactgc ggcaagctga gactgtgggg tgatttttcg tagttattta
    10201 aataatgaga acaggccgga gcgtaattca tacatccggc cttatttctt cagcttaacc
    10261 gagcgctaat tctgcggcag cggctttatc agcgattacc atcagcgaag gatgcagctg
    10321 caacacggaa gctggaacat cttcggtaac cggaccttgc agaacatttt tcagtgcctg
    10381 cgctttacct gcgccactga caattatcag caagtttttc gccgccatga tgcttttcgg
    10441 ccccatcgtc acgtagctgt caggcactaa cgaaaagtcg ccgcccaact caccatgcgc
    10501 cacaatatca accatctctc cctggatcgg gaacttcacg gtttgctcat ggaaatgggt
    10561 cgtattcggc aaattgccac aaaaatgacc atctgcacct aatcccagca ccaccaaatc
    10621 caggccgcct tcacgcgcca gtttctgatc atgctcgcgg tagttatcaa tagtgagctt
    10681 ctggatattc tcttctttga tccccgcagg ggtgaaaaac agattacgca gattggtaat
    10741 cgttacacct tctccctctt tgccgcgaaa gggaatttca tcgaaattat agaaatagca
    10801 gttatcgtac cagggcttac ctttaaccag ggtggtgagg tattcataca tgcctttggg
    10861 cgtgctaccg gcggtaattg ccaggttaac acgacgcatc ttcgacatat aaccaagcag
    10921 atggtgtgcc gcgacacggc tcatttcctg gtaatcttcg gtaatgatta atttcatctt
    10981 agtgccttat tttatttaaa taatttctgt acgaaggctg cataggcagg ccgccagaca
    11041 tccatttcat ggttcaggcc gggatattcc tggtaatcaa acttaatttt tttctgctca
    11101 agctcagttt tcagcccggc gatatccttg ccggttacga catctttatc accaacgacc
    11161 acagtaaaat tacgtagttg ctggttaata gctgccggat cgttcagccg ggccgcgaca
    11221 ccttcatccg gtacggttgt cgtggtaaca ccactgaatg tggccagcca gccaaagctt
    11281 tccagatgat tcattccgga aaccagcgcc tggtacccgc cttgtgaaag ccctgccagc
    11341 gcgcgaccat cggcatcttt acggacatta aaacgcttgc taatcagcgg gataatatcg
    11401 ttcatcagtt cgcgatctgc cgctttagca ttcagcggat aaaagacttt acgtctttcc
    11461 tgaggcacga aatcttcggg aataatgccc ttcgcatcgg tttcggtatc cgggatcacc
    11521 accagcatcg gtttaatttt cccttcagcc agcaggttat ccatgatttg cgggatacgc
    11581 ccctgatcga tagcggaacg tccggtatca ccaaaaccgt gatagaaata gagcactggc
    11641 aaaggctcgc ccatgccggt gtatcctggc ggggtccaga catacatctg acgttcagat
    11701 tgcaatgcgt tggagtggta agttatggcg atcaaatcac catgcgcaac agaacgcgta
    11761 tccagataac tgcctggcac cagaatcata ctggagttaa cctggcgctg aggcttagtc
    11821 attgcggtgc ctgtatcaat gctgcgtaca ccatcaacat tgaaaaaata ctcgtacagg
    11881 ttgcctttca ggataggtgt gcgccacgac cagactcctg cttcgtcttt ggtcatcggg
    11941 tgaatattgt ccggaaccgg aacacccacc actacagaga cattttttgc cccaggggca
    12001 aagtagcgaa aggtcacgct gttatcggca ttgacctgag tcacatactg tttcaccggg
    12061 atagtgggtg ccgggctggc tggcatatcc gctgctatgg cccactgagc agaaataccg
    12121 ctggcaatag ccagcgttaa agcagcaatt tttatattca tcgtaatatt cctttcatta
    12181 agttccatta ccaccagatt tcagcctggg caccgacagc aaactggtcg tctttattgt
    12241 cttcaaaggt gaagccatcc agttcgtttt ccagggcttt gatatacgtg gcgtagaaac
    12301 gaatttccgg acgcgaggtc aacatactgg tattgacttt aaaggtatgg aagagcgtgg
    12361 ttttataacc agactcatta aatttattac tattcgcatc tttgttttgc tgggtgaaat
    12421 agcccagttc aacacctgtc tggttatatt ggtcccaaat ataggccggg cgaacaaccg
    12481 cacgaataga ttcgaaatca gagtgggcgc ctgtttcgta gctatatata tcgttaccga
    12541 aggagtaaac aatcgcgtta gctacaatga aatgatcgcc aatataggct tcgccctgcg
    12601 aagtcagacg taccgctgtt ccgccggtgt gatcaccata ataacgacca ttaaaggtgg
    12661 taaatggact tgcgccagca taacggccaa agttactggc gatagaatta ttcgcgacca
    12721 ggaaggagaa ttcgttgaag ccacctttat caaatttctg cgttaaagat gtgccaaaca
    12781 tccaggtatc tttccagtca taatacccgt tattatcctg attatctttt tccgatgcgc
    12841 tttcgtttgc cgtgacataa cgaccactta ccattaaggt ggctttatcc cataacggga
    12901 tatctttata gcgtaaatca atggtattgg tattaatctg ctgtttgttt tgcaggctgc
    12961 gatcgtaatc atcgacatct tcgcgaacca gcgcgatatc aattttaccc ggaccgactt
    13021 tccagttttc cagacctaca cccgctgcgg catcagtacg ctgcgttttc cagtccagca
    13081 tctggatttc aattttcggc gcaccatgtt tacccaccca gaaatcagcc tctggcgcaa
    13141 agggcaggaa acctttagtg gtaacgtaca tatcggagaa ctgcatatag ttctcgccac
    13201 cggcgttatc gccaaaccag ccggtagagt actgctgacc aacgttacca tccatcatca
    13261 cgacggcatc aacccgtttg ccgttttcgt tgtagactcg ttgttttaac tgcaaatcaa
    13321 accagccgga gtattcgtta ccaaagcggc ccagagaacc aatcgcccat gatttaggtg
    13381 aaccatgaga ggcggtaccc caaccagaac ggtagtagcc gttgtaacta aagccaattt
    13441 catctttcac aaacttactg aaatctttca gagtcatgga agaataaatc gagcctgtct
    13501 cactggcatt tttctcttcg tttttgacca gtacagcatc aggttttgcg gcactactgg
    13561 ttgggaacgg attggcggca tacccttgct cattcgtact tacgctacga tttaccgtcg
    13621 ctggcgtata ctttttcttc tcttcatctt tatatttttt gagttcgctt tgcgtttctc
    13681 ttaacgcttt ttctaataac tcaaggcgtt gttccaccgt taatgattca gccaatgatt
    13741 gtgcagaaaa ggctaacggt gccattaata aaatggcaga ggtaataaga tttcgtctaa
    13801 acataatatc cctttatggt gcaaagaaag aattaacgca tcgcatccag actgttctga
    13861 atgcgatgat aattaaggtg ctttaatggt tattttttta atgacagccc ccgcgtcttg
    13921 atcacttctg cataccatcc gaagcttttc ttgcgcgttc ttgtgaggct tccttcgcca
    13981 ttatcatcac gatccacata aataaagccg tagcgcttag acatttgtga atgagatgca
    14041 ctgactaaat caattggccc ccaactggtg taccccataa tatccacacc atcggcaatc
    14101 gcttcattta cctgtaccag gtgatcgttt aaataggcaa ttcgataatc gtcctgtatc
    14161 gaaccatccg cttcaacgct gtcttttgcg cctaatccgt tctcgacaat aaataacggt
    14221 ttttgataac gatcccaaag cgtatttaac agaacccgta atccaaccgg atcaatttgc
    14281 cacccccact ctgaactttt cagatgcgga ttggggatca tattcagtat gttgccctgc
    14341 gcatttttat taatgctttc gtcgtgggaa acacaaccag tcatgtaata actaaaagag
    14401 atgaaatcga cggtatgttt taaatcttct gcgtcacttt cagtcatctc aatggtgata
    14461 ttgtggtcgc ggaagaaacg ctgcatatag ccgggatact ggccacgcgc ctgaacatca
    14521 ccaaagaaca tccagcgccg gttctcttcc atggcctgca acatatcctg tggctggcag
    14581 gtgagggggt aaaccagccc accgagaagc atattgccga ttttcgcttc ggggagcagg
    14641 ctatgacagg ctttaactgc ccgcgcactg gcaaccagtt gatggtggat agcctgataa
    14701 acttccgcct cgccactctc ttctgccagc cccacgcccg tgaatggctc gtgtaacgac
    14761 atgttgattt cattaaacgt cagccataac gccactttat gttggtagcg agtaaagacc
    14821 gtgcgggcgt aatgctcgaa gtgatcgatg accgctcgat tagcccaacc gccgtagttt
    14881 ttcaccagcc catatggcat ttcgtaatgg gataacgtta ccagcggctt gatccccgcc
    14941 tgcgccattt catcaaacag ccgatcgtaa aacgctaacc ccgcttcatt cggttcgact
    15001 tcgtcgccct gagggaaaat tcgcgcccag gcaatggaaa tacgcagaca ggtgaagccc
    15061 atctcggcaa ataacgcgat atcttccggg taacggtgat aaaaatcgat ggcgacatct
    15121 ttgatattct ctttccccag gatgcgcggt tccatttttc ccattacgcc atgaggctgt
    15181 aaatctgagg tcgagatccc tttgccatct tcctgccagg caccttccac ctgattggca
    15241 gctgttgcgc caccccaaag aaatgtttct ggaaatgctt tcataattaa ctccttttat
    15301 cgttagcgaa tgatggataa cagcggttca cctgcgctta tctgcgccgt gccgtggggt
    15361 aatacgtccg taaaatcatc gctattactg attaataccg gcgtcgtcag atcaaatccg
    15421 gcctcgcgaa tagcagggat atcaaaagaa atcagccgat cgcctgtatt gaccttgtca
    15481 cccacgttga cgtgagcgga aaagaatttg ccgtccagtt ttacggtgtc gataccgaca
    15541 tgaatcagga gctccacacc atcatctgac tcaatgccaa tggcgtgtaa tgtggcgaac
    15601 aacgaagcaa ttcgacccgc aaccggagaa cgcacttcac caaccgaggg cagaatggcg
    15661 atacctttac ccaacaggcc actggcaaac gtggtatcag cgacgtgaat gagcggcaca
    15721 atctctcccg tcatcggtga acagataccg ccctgctcag gtggtgtaat aacctctggt
    15781 gttttctctt gcggggcacc ctgcgctggc tgacgtttag cggtgatgaa atgaagcatc
    15841 accgtaccga caaatgcgca accgatggca atgacaccgc caataacgct ggcccagacg
    15901 gtgaaatcaa ttcccgttga cgggatggtt tgcatgaagg tgaaaatact tggcaaacca
    15961 aaggagtaga ctttcgtttg cgcgtagcca atgatggtgg cccccaaagc cccactgata
    16021 caggcgataa caaaggggta cttacgcggc agattaacgc catataccgc tggttcggtg
    16081 ataccaaaca ggcccgtcaa cgccgctgat cccgccacca cttttttctg cgcatcgcgt
    16141 tcgcagagga agacgccgag cgccgccccg acctgcgcca taatggcggg cattaacagc
    16201 gggatcatgg tgtcgtagcc cagcacggtg aagttattga tacatagcgg caccaggccc
    16261 cagtgcagtc cgaacatgac gaagatttgc cagaagccgc ccattaccgc gcctgcaaat
    16321 gcaggaaccg cctgataaag ccagagataa ccggcggcaa tcagttcgct tatccaggtt
    16381 gatagcggcc ccaccagcag aaaggtgacg ggtgtgataa ccatcagaca tagcaatggt
    16441 gtgaagaaat ttttgattgc cgacggtaac cacgcattaa gtcggcgttc cagaatgctg
    16501 cacaaccagg cagaaaaaat aatgggaata accgatgacg agtaattcaa caatgtgacc
    16561 ggaataccca ggaaatccag ccccagctca tccgcttttt gcccgttctc gaaagcagtc
    16621 agaattaatg gatgcactaa cgctccacca atcaccatgg cagtaaatgg attaccgccg
    16681 aagcgtttcc ccgcggtgta tcccaggatt atcgggaaga accaaaacaa ggcatcactg
    16741 gcgctgaata aaattaaata agtaccactt tgttcggtcg tccactgaaa agtgagcgcc
    16801 agagccagca tacctttcag gatcccggtt gccgccatca aaccgatcag aggcgtaaaa
    16861 atacctgaaa taacataaac aaagcggttt agcagattac ctttctcatc attttccggt
    16921 gcctgttgcg ctttttcgcc aaggcctgcc acgctgttga ccgccaggaa gacatcggcc
    16981 acatggttac ctatgaccac ctgaaactgg ccaccgcttt ccaccaccat aataataccg
    17041 ggggtgtttt tcagtacctc agcttgcgct ttgctttcat cctttaactt aaaacgtaat
    17101 cgcgttgcgc aatgcatcag actaacaatg ttatctgcgc ccccgactcc tgcgactatt
    17161 tttctggcta actccgtcat aacttgccct ctaccgcttt gcggcaaaac tcaaaaaaaa
    17221 acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt caggttttgc ccgcttagtg
    17281 cggtaacaat cctttactca gtaataatat ttcagtgttc tttgcgcacg cgctctatat
    17341 ttatggctaa aaacataatc tctgcgggtg aaattttacg ttgatactgc aaaccaataa
    17401 aaatggcgat ccgttccgca cattgccatg cttgcgggta attttgtttt actgcttgtt
    17461 gtaatgattc atcactatcg ttaattgaag catgttcaag aatacgccag gataaaaact
    17521 tcagatgtgt aaccagtcgc tgataactca agctttcttc ctggtaatta aggctgaact
    17581 gaaattttat taattgcagc atttcgcgca ttaactgcgt gacacctgca acatcctcca
    17641 tatttccgct catttgggca ctgaccagat gcatggcaat aaagcccact tcatctttcg
    17701 gtaactgcac gcccaaccgt ttatcaataa tggttaacgc ttcttcccct agctggaact
    17761 ctttcgggta aagccgctgg atatcccaca gcaacgggtt gggcagcaac acgttttgct
    17821 gaaagcgttt aatcgcaaac tggcaatggt cagttagcga gatataaata ctgtcctgta
    17881 attttcccaa gcgctcctgc gctaaagaga taatacgatc acaggttgcc atcacctcaa
    17941 gaggaatatg acttaagagt tcgcttaatc gcccgttcag ttcatgactg ctcaaggcat
    18001 actctttttc tattccactt gagttaattc tttcgccagc gcgtttttga aagccaattc
    18061 ctcgccccat gacgactttt tcccgctgtt gatcatcaat aaccaccaca acattattgt
    18121 tgagaatttt ggtgatttgc atgttcatag caaggacctt ttttataaac aaaaaaaccc
    18181 gacttcacca gtattctctg gttatgtcag gttttgcctg
//
LOCUS       DAZFRN010000093        18110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFRN010000093 DAZFRN010000000
VERSION     DAZFRN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.093"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(3..761)
                     /gene="aroD"
                     /locus_tag="ACHKUB_003129"
     CDS             complement(3..761)
                     /gene="aroD"
                     /locus_tag="ACHKUB_003129"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO6656109.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(792..1658)
                     /gene="ydiB"
                     /locus_tag="ACHKUB_003130"
     CDS             complement(792..1658)
                     /gene="ydiB"
                     /locus_tag="ACHKUB_003130"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO6656110.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNIDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1670..2935)
                     /locus_tag="ACHKUB_003131"
     CDS             complement(1670..2935)
                     /locus_tag="ACHKUB_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6656111.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3162..4376)
                     /locus_tag="ACHKUB_003132"
     CDS             complement(3162..4376)
                     /locus_tag="ACHKUB_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6656112.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPRTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4476..4832)
                     /locus_tag="ACHKUB_003133"
     CDS             complement(4476..4832)
                     /locus_tag="ACHKUB_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO6656113.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4968..5075)
                     /gene="rprA"
                     /locus_tag="ACHKUB_003134"
     ncRNA           complement(4968..5075)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKUB_003134"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5261..6373)
                     /gene="ydiK"
                     /locus_tag="ACHKUB_003135"
     CDS             complement(5261..6373)
                     /gene="ydiK"
                     /locus_tag="ACHKUB_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO6656114.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6762..9818
                     /locus_tag="ACHKUB_003136"
     CDS             6762..9818
                     /locus_tag="ACHKUB_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO6656115.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9815..10225
                     /gene="menI"
                     /locus_tag="ACHKUB_003137"
     CDS             9815..10225
                     /gene="menI"
                     /locus_tag="ACHKUB_003137"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO6656116.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10325..10513
                     /locus_tag="ACHKUB_003138"
     CDS             10325..10513
                     /locus_tag="ACHKUB_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO6656117.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11061..11429
                     /gene="sufA"
                     /locus_tag="ACHKUB_003139"
     CDS             11061..11429
                     /gene="sufA"
                     /locus_tag="ACHKUB_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO6656118.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11438..12925
                     /gene="sufB"
                     /locus_tag="ACHKUB_003140"
     CDS             11438..12925
                     /gene="sufB"
                     /locus_tag="ACHKUB_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO6656119.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12935..13681
                     /gene="sufC"
                     /locus_tag="ACHKUB_003141"
     CDS             12935..13681
                     /gene="sufC"
                     /locus_tag="ACHKUB_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO6656120.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13656..14927
                     /gene="sufD"
                     /locus_tag="ACHKUB_003142"
     CDS             13656..14927
                     /gene="sufD"
                     /locus_tag="ACHKUB_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO6656121.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAQLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14924..16144
                     /gene="sufS"
                     /locus_tag="ACHKUB_003143"
     CDS             14924..16144
                     /gene="sufS"
                     /locus_tag="ACHKUB_003143"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO6656122.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16157..16573
                     /gene="sufE"
                     /locus_tag="ACHKUB_003144"
     CDS             16157..16573
                     /gene="sufE"
                     /locus_tag="ACHKUB_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO6656123.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16722..17726
                     /gene="ldtE"
                     /locus_tag="ACHKUB_003145"
     CDS             16722..17726
                     /gene="ldtE"
                     /locus_tag="ACHKUB_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO6656124.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17790..18026)
                     /gene="lpp"
                     /locus_tag="ACHKUB_003146"
     CDS             complement(17790..18026)
                     /gene="lpp"
                     /locus_tag="ACHKUB_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO6656125.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4743 a         4392 c         4658 g         4317 t
ORIGIN      
        1 gcttatgcct ggtgtaaaat agttaatacc gtgcgtaaat catttaccga gatttgccct
       61 ggtgcagagg ctttttttac cgcaccaaaa gttgccgccg agccaaatac ttcaccagcc
      121 agacgagaaa ttacgccagt ttttgccatc gacatcgtga tgattggacg atcggcatac
      181 tgctcctgca tctccagggt cgcggtaagc aacgtcagca catcgctggt actttgcggc
      241 atcagcgcaa tcttaggaat atcggcgtcg aaggactgca ttttgcgcag acgggcaatg
      301 atttcttcgg cttccggcgt tttatggaag tcatggttgg acatgacaac tttcacatca
      361 tgcgcgtggg cgtaggcgac ggtttcttta acctgatcat cgccggtaaa taactccaga
      421 tcgatcatat caaccaggcc gctgtcgatg gctgcacgat tgagagcaat ataagcctcg
      481 gtggaaatcg cctgctcgcc gccttctttg gcactgcgga aggtaaacag cagcggtttt
      541 tctggcatgg tttcacggag aatttttgcc gccgccatga cagactccac attggagagg
      601 tcggcaaagt ggtccacacg ccattccaga atatcaaagt ccgcttcacg ataggcgaga
      661 gcttcggatt tcacgcgggc gatatctttc gccatcagcg agacgatgat tttaggtgcg
      721 cccgtaccaa tgacgagatc ttttacagtt acggttttca ttttttaccc tttctgcacg
      781 cggtcagcct gtcaggcacc gaaccccatg acctgtttaa catattccag agggaaatct
      841 ttgccagtcc ataacgtgaa ctgttcagcc ccttgccaca acaacatgcc gtatccatca
      901 atcgttttgc aaccagcttg ttgcgcctgc tgcaataact tcgtcatatg cgggttatac
      961 acgcattcag tgaccagcag tcccggatgt aacagactga tatcattaac taatgattca
     1021 ttctcaaggg gtttcatacc cacttttgtg ccattggtta aaatgtcggc ggaagccagg
     1081 gcttcagcaa aggcttgctg atcggcgaga tcggtaaccg tgacgacaca atcggtgttt
     1141 tcgttaaccc gctgcgcgaa ggcgagggct ttatcgaaga actcatcccg acggttaaag
     1201 agtttaattt cttttaaacc ttcaattgcc ccctgcgcgc caatcgccgt tgaggcacct
     1261 ccggccccta acagcaccat tgttttgcct ttgatatcaa aaccgctctc tttaatggcg
     1321 cgaatatggc ccgtgccgtc gatgttatag ccgcgcaggt agccatcatc attaacgatg
     1381 gtgttgatgg cacccaccag tttggctgct ggcgttaatt catcaacata ttcacacgcc
     1441 agttgtttgt tcggcattga tataccagtt ccgcgcattt tgagggcttt taatccttca
     1501 attgctccgg gaaaactatc gttatccact tcaaaggcca tataggtaaa tggcaatccc
     1561 gctttttcta aggctttatt ctgcatttca ggcgataaac tgtggcggat aggataggcc
     1621 atcagcccaa tcaattcgta ttttgcggta acatccataa ttaactcctt taacctctat
     1681 gcttaattgt gtttaaccgg gtgctaaatt tgcgctcgcc aaaccgcaca tcattctcag
     1741 gaataataaa aacgcggtaa tagcggataa aaacaataat tgcggtaatc agggccagca
     1801 gcgcgaaagt aaaatcgaga acaatgatat attgcaggcc gatgttcgac agataaccgg
     1861 taatcagcgg aataacaaag ttagccagtc cacccatcat catataaata ctggtgactt
     1921 tggctttgct ttttgggaaa aactctgaca tgaccgaaac accgagctgt aaaatgccgc
     1981 cagctgctga gaaaccgata acaaaggcgc cggcattaca caccagtgga gaagggtaca
     2041 ggtagataat ggctgctgtt attgttgcca gtgcagagtt aaatacatta gcccagatgg
     2101 gccggaccat ttttttcagt agtgcggcaa aaataaagac acagaccaac gagcccatac
     2161 tgtaataaga gatggttttt aatgcctcag cttctgacat accagcaaaa gccatcgcat
     2221 atttgggcat ccacaccaca atcacataaa aggtcgagaa tgcggctaca ccgaacagta
     2281 ccgatgaaac accttccagc cagactaacg gtttgctgtt catttgcggt aattcattag
     2341 ctacgctggc gtccaccaac tggctgggga atttgctttt caacagcatc agcgtgatca
     2401 gtacaaacag aatacccgga ataatcagcc catagccgta ccagatatta ttgagcaaca
     2461 tatagctcac cagcattggg tagaacattt gcccaaatga caccatcgct ttaaccagta
     2521 tgaccgccga accagaggct ttcggaaagc attccatgag cgcggggtag ccacccgtat
     2581 ccagcgctga gttagcgata cctacgcaca ctgccagacc gtaggcgaga gttaaattcg
     2641 ggcaagcggg aataccaaag aagaatagca gatacattat tactgccatt aatatcaccg
     2701 cccgacgacc aaacttatcg gagatcacac cgaagaataa aatactgatc aatcgcccca
     2761 aaccaatacc ggaaattaag taggcaatgc ccgcgttgtc agtggaaaac ttttccgcca
     2821 gagatgacat gttttgggca agcgtaataa cactaatgcc gtgcaggaag tagctgaagt
     2881 aaatacaaag aacagccagg ataaatggcg tgctgaaagc cttattttga gacattttca
     2941 cagctccttg ctgaaagata atttcgttac cgtacgtatt gttcttcgac ggtttacata
     3001 attactatac tgaagatgat tttccctggg tacatggcat aaagtttatg tttccaggaa
     3061 tattgcatgt catagtttgt ttttgatcgc gattttcctt agttagcgca gaaagagtta
     3121 tttatccgta agatcttgca tgacctgaaa agaatattgc attacccacc cggagcgaca
     3181 ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc gtaagccgat aaacagtgcc
     3241 agtaaaaaac cgatggcagc gatggcggta tcgaaccaca taatatcggc aatacttctt
     3301 tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc cgcactgtaa
     3361 tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa cgtcaggcca
     3421 atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat tatcaccaca
     3481 taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa ggtgtacagc
     3541 atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat aaatacacaa
     3601 agcagcgaac ccacggtgta gatactgagt agtttaatcg acatagtgta tgacatgcct
     3661 gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt tgccattgag
     3721 atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg ttccgtagag
     3781 ctggtggttt tctttatgac aggtaagcga tgacccggat ggggtgggaa cgtacaacgg
     3841 tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga ccaaccgaac
     3901 cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg tccgctggaa
     3961 acaaatgctt taattaaaat attggctgtc ctaggtgagc gtggaaaagc ttccatcaaa
     4021 ctgggataag tgcctgcatc gagaaaactg tttgccattc ctgccagaaa gccaaaaaca
     4081 taagcgataa tgatgttatt ggtgtgcagg atgccaaaaa agaaggccat atagcagcac
     4141 atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc tgcaaaaagc
     4201 aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc cgcggcatta
     4261 gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac gcccatacca
     4321 tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt tttcataggt
     4381 tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc cattcattat
     4441 tgttgtaaca acgacaattc ctttctgtgg aaaaattagc aaagccgctc cagatcgtgt
     4501 gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt gccatcaggg
     4561 tagactcgtt gaaatgcggt taagtcgggg aaaaaacgca tggtgttgtt gccgatgcgg
     4621 ttatttattt tctctatgat ggcatgaaga tgtttttttc tttgctgacg catagccaac
     4681 agttgctcga ctacacgatc aggaatggca cacttgccat tttcccattg acgccagctt
     4741 tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat ggcaaaaata
     4801 tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga aataaacaat
     4861 aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag tatcttgtgc
     4921 aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta aaaaaaagcc
     4981 catcgtggga gatgggcaaa gactacacac agcaattcgt tgtttcactc aggggatttc
     5041 catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc atacggactt
     5101 tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg cgacaggtac
     5161 taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat atcaattagc
     5221 ggtggttaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc ggtttctcga
     5281 tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggcggcggg acttcttcca
     5341 cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata aacagaccga
     5401 tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc agcggtaaat
     5461 cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca accacaccgc
     5521 tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc cagataatcg
     5581 ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc attaacaccg
     5641 ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca agcactgcct
     5701 gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt gccgccagca
     5761 gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga atgccttgtg
     5821 ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc gcacaatgca
     5881 ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg gtgccaatat
     5941 aagggcggac tttcgccatg atcgccgtcc cccccatatc cagcaagttg tgccagcctg
     6001 catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg ggtaacgtca
     6061 tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata ctgttaacca
     6121 gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc agaacggcga
     6181 gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag gtggcgataa
     6241 ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac agacatgcca
     6301 caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc ctgggctgac
     6361 gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga cgcgacgtga
     6421 acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac aggccgattc
     6481 tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc aggcaacacg
     6541 caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc gttcacccca
     6601 gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg caactcgaaa
     6661 tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg aaagaatagt
     6721 gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca cagatttccc
     6781 aggcacccgg cgtcgttcaa ctggtgctta attttttgca agagctggag caacaaggtt
     6841 ttaccggcga tacggcgaca agttatgccg atcgtctgac aatgtcgacc gacaacagta
     6901 tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat gtggcgctga
     6961 tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc cgcggcggcg
     7021 gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg tcccgccata
     7081 tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag gccggggtga
     7141 taaaagatca actcaatcag tacctgaaac cgttcggcta cttttttgcg ccggaacttt
     7201 cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc ggtcagggat
     7261 cgctggtcta tggcaaaacg tcggatcacg tacttggcgt acgcgcggtg ttgttggggg
     7321 gcgatattct cgatacgcaa cctttacccg tcgaactggc ggaaacgctg ggtaaatcca
     7381 ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag caacgccagt
     7441 taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat ctgcgtcatg
     7501 tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt tcagaaggga
     7561 cgctggcctt tattaccgaa gcgcggctgg atattacgcc cttgcctaaa gtgcgccgtc
     7621 tggtgaacgt caaatatgac tcttttgact ccgcgctgcg taacgcgccg tttatggttg
     7681 aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg gcgcgggaag
     7741 atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa gagatgctcg
     7801 ggctgaacat tgtggagttt gctggtgatg atgaggcgct gattgacgag cgagttaatg
     7861 ctctctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg attggctggc
     7921 aggtgtgccg cgagctggcg ggtgttgaac gtatctatgc gatgcgtaaa aaagccgttg
     7981 gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag gatacctgcg
     8041 taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc gacagccacg
     8101 gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc cgtccagcgc
     8161 tggatatgtg cgatcctcaa caagagattt tgatgaagca aatctctgat gacgtagtgg
     8221 cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt tttcgcgctg
     8281 aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa gtgaaagcgg
     8341 catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa ggtctcgatg
     8401 cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag atccccattg
     8461 cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta tgcttcaact
     8521 ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg attcattcac
     8581 cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgttt gttggcggat cgcggcgttg
     8641 atccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg acgttaattg
     8701 cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca cacgaagtca
     8761 aagaggcgat gtcgggctgt ctggcctgta aagcgtgttc gacccagtgc cccatcaaaa
     8821 ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt tatttacgcc
     8881 cgctgcgcga ccacctcgtc gctacggtcg agagctacgc gccgctgatg gcacgcgcgc
     8941 cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg gaaaaacata
     9001 tcggcatggt tgatttgccg ctgctgtcgg tcccctcgct acaacaacaa atggtggggc
     9061 atcgctcggc aaacatgacg ctggaacagc ttgaatctct caatgcagag cagaaagcgc
     9121 gcacagtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa gtggtggcgg
     9181 attttgtccg tctggtcgaa aaattaggtt tccagcctgt gttactgcca ttttcgccaa
     9241 atggcaaagc ccagcatatt aaaggttttc ttaatcgttt tgcgaagacg gcgaaaaaga
     9301 cggcggattt cctcaaccgt atggcgaagc tgggtatgcc aatggtgggc gtcgatccgg
     9361 cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa cgtggcgagt
     9421 ttaacgtctt actggcgaat gaatggctgg caagcgcact tgagtcacag ccagtggcta
     9481 cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaagttacc gccttgccgg
     9541 gtgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg gaaaatgtca
     9601 gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac cataaaaatt
     9661 cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg cgtaaccgct
     9721 gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc acgggggtac
     9781 gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga aaatcaccct
     9841 ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata ttcgctttga
     9901 acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga caaagcagcc
     9961 tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg gttccgttgc
    10021 cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca atgctaacca
    10081 cgtccgctcg gcacgagaag ggcgggtgcg cggggtatgc aaaccgttgc atctcggttc
    10141 gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt gctgttcgtc
    10201 acgattgacg accgccattt tgtgacgttg tcaccagaaa agtgtgacgc atattgcctt
    10261 tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta aaagcgagga
    10321 tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac gccgtgacca
    10381 gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac ttgaatttgc
    10441 tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg cctggcgtct
    10501 gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg gattaacctc
    10561 cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt gttcaaaaat
    10621 aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta ttcttatcgc
    10681 cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg gtagaaaccc
    10741 ttaagcctgt gttgcacaga cttaagggtt ttcttatttc tgaacgctgc acttttttac
    10801 ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag ccttaatgct
    10861 ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg cctgttaacc
    10921 cacacatcag ggtctatgct tattaaaagc aggcaaaaag catgttttac ggtaaagccc
    10981 ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat acgctgaaag
    11041 cgatgaagtg aggtaaatcg atggatatgc attcaggaac ctttaaccca caagatttcg
    11101 cctggcaagg cttaacgctg acacccgcag cggcgataca catccgtgag ctggtggcaa
    11161 agcagccggg tatggtcggc gtgcgcttag gcgtgaagca aacgggctgc gcgggctttg
    11221 gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt gaacacgacg
    11281 gcgcgaagct gtttgtcccg ctgcaagcga tgccgtttat tgatggcacg gaagtcgatt
    11341 tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc cagaatgaat
    11401 gtggctgtgg cgaaagcttt ggggtatagg cggtactatg tctcgtaata ctgaagcaac
    11461 tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat tcttcaccca
    11521 gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg caatttcggc
    11581 gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc gcgcatggct
    11641 ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt atcaggatta
    11701 cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt ctgaacctgg
    11761 cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg cggcgtttga
    11821 gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca ttttcgactc
    11881 tgtttcggtt gccactactt atcgcgaaaa actggcggag cagggaatta ttttctgttc
    11941 ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg gcaccgtggt
    12001 gccggggaat gacaacttct ttgccgcgct taatgcggcg gtagcctctg acggtacgtt
    12061 tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt ttcgcattaa
    12121 cgcggaaaaa actgggcagt ttgagcgcac cattctggtg gccgacgaag acagctacgt
    12181 cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttac acgcggcagt
    12241 ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac aaaactggtt
    12301 ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg ctttgtgcga
    12361 aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga ttacgtggaa
    12421 atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact cagtggcgct
    12481 gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg gtaaaaacac
    12541 caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca gttatcgcgg
    12601 cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt gcgactcaat
    12661 gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc gcaataacag
    12721 cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac tgttttactg
    12781 cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg gtttctgcaa
    12841 agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac tcctcgccat
    12901 cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag atttacacgt
    12961 cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc ccggcgaagt
    13021 tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa cgcttgccgg
    13081 gcgagaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag atttgcttgc
    13141 gctgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt atccggtgga
    13201 gattcccggc gtcagtaacc agtttttcct gcaaacggca cttaatgcgg tgcgcagcta
    13261 tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag agaaaatcgc
    13321 tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt tttccggcgg
    13381 cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt tatgcattct
    13441 tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg atggcgtgaa
    13501 ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac gcattctcga
    13561 ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga aatccggcga
    13621 tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg aacagcagta
    13681 atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc tcaccgcaag
    13741 cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa catgaaaact
    13801 ggaaatatac gccgctggaa gggctgacca atagccagtt tgtcagcatt gcgggagaga
    13861 tatccccaca gcagcgagat gccttagcgt taacgttaga cgctgtgcgg ctggtatttg
    13921 tcgatggacg ttacgtgtca gcactgagcg atgcgactga aggcagcggg tatgaagtga
    13981 gcattaacga cgaccgtcag ggggtacccg acgctattca ggcggaagtg tttctgcatt
    14041 tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt caacggccgg
    14101 caaagccatt gctgttaatg catattaccc agggcgtggc aggtgaagag gtcaacactg
    14161 cccattaccg acatcatctg gatctggcgg aaggtgccga agcaacggtg atcgaacatt
    14221 ttgtcagcct taatgatgct cgtcacttca ccggcgcacg gttcactatc aatgtcgcag
    14281 cgaacgccca attgcagcat atcaagctgg cgtttgaaaa cccggtcagt caccactttg
    14341 cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt ttcctgctgg
    14401 gtggcgcagt gttacgacac aacaccagta cgcaactcaa tggcgaaaac agcacgctgc
    14461 ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt acctggctgg
    14521 aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc agcgacaaag
    14581 gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa acggatggtc
    14641 agatgaccaa caataatctg ctgatgggca aactggcaga agtggatacg aaaccgcagc
    14701 tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg cgtattgatg
    14761 atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc cagcagatga
    14821 tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg cttaaacagc
    14881 aggtgctggc ccgaatcggt caacgactgc caggaggtgc aagatgactt tttccgtcga
    14941 caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc cgctggctta
    15001 tctcgacagc gccgccagtg cgcagaaacc aagccaggtg attgactccg aagccgagtt
    15061 ttatcgtcac ggctacgcgg cggtgcatcg cggtattcat accttaagcg cccaggcgac
    15121 cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc gttcggcgga
    15181 agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca atagttgggg
    15241 caacagcaac gtgcgggcgg gcgataacat catcatcagt cagatggagc accacgctaa
    15301 cattgttccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg tgatcccgct
    15361 caatcccgac ggtacgctgc aactggagac gctacctaat ctgttcgatg agaaaactcg
    15421 cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatcccc tggcggaaat
    15481 gatcacgctt gcgcatcagc atggcgcaaa agtgctggtg gatggcgctc aggcggtgat
    15541 gcatcatccg gtggacgttc aggcgctgga ttgcgatttt tacgtattct ccgggcataa
    15601 actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt tgcaggagat
    15661 gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg aaggcactac
    15721 ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg gcatcattgg
    15781 tcttggcgcg gcgctggagt atgtttcggc gctggggctt aataacatag ccgagtatga
    15841 acagaatctg atgcactacg cgctatcaca gctggaatct gtaccggatc tcactctcta
    15901 tggcccgcgg aacaggcttg gcgttattgc ttttaatctt ggtaaacacc acgcctatga
    15961 tgttggcagt tttctcgata actacggcat tgctgtgcgt accggacatc actgcgctat
    16021 gccattaatg gcctattaca acgtccctgc tatgtgtcgg gcgtcgctgg ccatgtataa
    16081 cacccatgaa gaagtggatc gtctggtgac cggactgcaa cgtattcacc gtctgctggg
    16141 ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc gtaatttttt
    16201 acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc agcgtctgcc
    16261 agaattacgc gccgaagacc gaagcccaca aaatagcatt cagggctgcc agagtcaggt
    16321 gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg acagcgatgc
    16381 ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga tgacgccgca
    16441 ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca cccaacatct
    16501 caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg ccaaagccgc
    16561 tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg ccggatggtc
    16621 aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa gcgccatccg
    16681 acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc gcgtctttgc
    16741 ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg gttgattatc
    16801 cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg gtgcaagaag
    16861 gggataaaaa ccttcaggct attgcccgac gttttgatac tgcggcaatg ttgatccttg
    16921 aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact attccttcac
    16981 aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca gagctgcgcc
    17041 tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt attggattgc
    17101 aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct aacccaacct
    17161 ggacgcctac ggcaggcatt cgtcaacgtt cgctggagcg tggcattaaa ttaccgccag
    17221 tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc gcgcatggta
    17281 atggcgaata cctcattcat ggtaccagtg cgccggacag cgtcggtttg cgcgtcagtt
    17341 cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc gtgcggacgg
    17401 gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct aacgggatgc
    17461 gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt cagacaatgc
    17521 catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta gatcagaagt
    17581 tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc agtggagcaa
    17641 ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa ccagagcaag
    17701 ggaatatgtt acgcgcgacg cagtagcggt aaacggcaga caaaaaaaat ggcgcacaat
    17761 gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc catgttgtcc
    17821 agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga acgcattgcg
    17881 ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc agaagacagc
    17941 tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag gattaccgcg
    18001 cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt aatctccatg
    18061 tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
//
LOCUS       DAZFRN010000094        18099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFRN010000094 DAZFRN010000000
VERSION     DAZFRN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.094"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(231..1823)
                     /gene="lsrK"
                     /locus_tag="ACHKUB_003147"
     CDS             complement(231..1823)
                     /gene="lsrK"
                     /locus_tag="ACHKUB_003147"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO6656126.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1902..2855)
                     /gene="lsrR"
                     /locus_tag="ACHKUB_003148"
     CDS             complement(1902..2855)
                     /gene="lsrR"
                     /locus_tag="ACHKUB_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO6656127.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3104..4639
                     /gene="lsrA"
                     /locus_tag="ACHKUB_003149"
     CDS             3104..4639
                     /gene="lsrA"
                     /locus_tag="ACHKUB_003149"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO6656128.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTNDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETFYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4633..5661
                     /gene="lsrC"
                     /locus_tag="ACHKUB_003150"
     CDS             4633..5661
                     /gene="lsrC"
                     /locus_tag="ACHKUB_003150"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO6656129.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5661..6653
                     /gene="lsrD"
                     /locus_tag="ACHKUB_003151"
     CDS             5661..6653
                     /gene="lsrD"
                     /locus_tag="ACHKUB_003151"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO6656130.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6665..7687
                     /gene="lsrB"
                     /locus_tag="ACHKUB_003152"
     CDS             6665..7687
                     /gene="lsrB"
                     /locus_tag="ACHKUB_003152"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO6656131.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7714..8589
                     /gene="lsrF"
                     /locus_tag="ACHKUB_003153"
     CDS             7714..8589
                     /gene="lsrF"
                     /locus_tag="ACHKUB_003153"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO6656132.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8613..8903
                     /gene="lsrG"
                     /locus_tag="ACHKUB_003154"
     CDS             8613..8903
                     /gene="lsrG"
                     /locus_tag="ACHKUB_003154"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO6656133.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8960..9718
                     /gene="tam"
                     /locus_tag="ACHKUB_003155"
     CDS             8960..9718
                     /gene="tam"
                     /locus_tag="ACHKUB_003155"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO6656134.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENEQILLAFPRLFIVARRTE"
     gene            complement(9722..10636)
                     /locus_tag="ACHKUB_003156"
     CDS             complement(9722..10636)
                     /locus_tag="ACHKUB_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO6656135.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10843..12297)
                     /gene="uxaB"
                     /locus_tag="ACHKUB_003157"
     CDS             complement(10843..12297)
                     /gene="uxaB"
                     /locus_tag="ACHKUB_003157"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO6656136.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVVLDLPR
                     DELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTF
                     ALAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHW
                     EQDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12524..13942)
                     /locus_tag="ACHKUB_003158"
     CDS             complement(12524..13942)
                     /locus_tag="ACHKUB_003158"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6656137.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14081..14440)
                     /locus_tag="ACHKUB_003159"
     CDS             complement(14081..14440)
                     /locus_tag="ACHKUB_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO6656138.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14440..15366)
                     /gene="glsB"
                     /locus_tag="ACHKUB_003160"
     CDS             complement(14440..15366)
                     /gene="glsB"
                     /locus_tag="ACHKUB_003160"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO6656139.1"
                     /translation="MAVAMDNAILGNILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15430..16818)
                     /gene="sad"
                     /locus_tag="ACHKUB_003161"
     CDS             complement(15430..16818)
                     /gene="sad"
                     /locus_tag="ACHKUB_003161"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO6656140.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16919..17800
                     /locus_tag="ACHKUB_003162"
     CDS             16919..17800
                     /locus_tag="ACHKUB_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6656141.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17878..>18099
                     /gene="yneK"
                     /locus_tag="ACHKUB_003163"
     CDS             17878..>18099
                     /gene="yneK"
                     /locus_tag="ACHKUB_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO6656142.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSF"
BASE COUNT         4537 a         4628 c         4503 g         4431 t
ORIGIN      
        1 attaaacccc ttttgcatcc attagtgtct ttaggaatat tcgttataaa ataagcatta
       61 tgcttaatgc tttaagaaaa atagcaattt ccccttgaat atattactgg tgatggaagg
      121 atgaatctgc aagctggcat ttaacaagcc agttctaaaa gaagggaaat aagaataact
      181 atactcaaaa actaacagcc acggtcatca tgatgtggct gtcaatgaaa ctataaccca
      241 ggcgctttcc ataacgacgt cgtcagtcca tgatcaacca gccccagctg atcctgataa
      301 actgcctgcc acttatcgcg tgaatcctga taaagttcat gcttttccgg gtctggtgtg
      361 tgcgtccgtt cccagcgaac caggcgttct ccggtttctg ccattgatga aaaaattccg
      421 gcaccgacgc cagctgcaat ggcacatcct aatgcagtgg cttctttgac caccggaata
      481 ttgacgggta atcccgagac atcagcgaga atttgactcc ataatttccc ttttgaacct
      541 ccgcctgcaa agactaacga tgaaggatga atattcgaga aatcagcaat ttgctgcaag
      601 ttacacgctg atacaatcgc cgcattttct tccagcgcac ggaacaatgt cgctttgtta
      661 catttatccg ggtcaatgga caagttaata aaggaaggcg cagcgtgata ccaggtttta
      721 aagcgcattc tgtcggagaa tatcggcatt acgccccacg acccaggcgg cacccggctg
      781 gccatctctt ccaacagcgt ataggtgtcg atgcctagac gttcggcaat cagtttttct
      841 tcggcacaga aagcatcgcg gaaccagcgc atggtgagtc cggtaaaaaa gcttatagat
      901 tcagcttgta ccatgccagg aataacatgg ggattaacgc gcacgttcat ttctggatcg
      961 gtcaccggcg cggctaagtt tacaacttgc tgccagaatg tgccgccaag aacagcggtt
     1021 tgtgccggac gcacaacgcc taaccccagg caaccaagct gcacgtcacc tcctccaacg
     1081 accaccggag tgcccgcctt cagaccgcag agttccgccg cttgtgaact caccacaccc
     1141 agcaatgtgc cggtttcttt gacaggagaa agaatatcgg cacgcaggcc agccatatcc
     1201 agcaatgcag gtttccagtc acgggtggtt agatcaagaa gtcccgtggt gccagcgtta
     1261 gagggatcca ccgcaagttc gccgctgagc atataggcca gccagtcgct gatcatggtg
     1321 atggttgatg cctgacggta aatatcggaa cgatggtgcg ccagccaaag taatctgggg
     1381 atggcactta aagccagtgt ttgtccggtc gcgcgataaa cttcgttttc aaaggtattg
     1441 ttgtgcagtt ctttaagttc gctaacttcg cgcgccgctc tggcatccac attggcgcag
     1501 gcccagatcg gtgttccttc attgttatat aaaacaatgc cttcacgcat cgaacatgcc
     1561 gaaacggcag cgatatactc cggggctatg ccggcgttgt gcaacgcctg gcgcatacac
     1621 tcacacgcca gttgccagtt tttgttgaga tcaaattcca tagaaccagg aacgtccggt
     1681 actgccagat gccgccactc cgcctgtccc actgctattt gattgccttc caggtcgaat
     1741 atcacagccc gaatacttcc ggtgcctgca tccagcgcca tcaggtagta ctttgattct
     1801 gaaggggtaa agagtcgagc cattagatta tcctcggctt atgcgttctg tctgttcctc
     1861 tatacgttct ccatcattcc cggtaataag gtcatgcaaa tttaactacg taaaatcgcc
     1921 gctgctgtgt cctgatcggt aaccagtgcg ttgatataac cgcctttcat tgcagcggca
     1981 attgcttcgg ctttattttc tccccctgcc acgccaaccc ggacgggtat ggtcttcagc
     2041 gcgcttaaag gtaagccaat cagttcgtta tgtattttga tatccgtgac aacgtcacct
     2101 ttcgcatcaa aaaagtagcc taaaatgtcg ccaaccgccc cttttcggcc aatcattaac
     2161 tgttcgccct ggctgatata accggagcga atgattgtcg cgtcgtcttg ttgactcacg
     2221 gccccaatgc cgacaatcgc cacatccgct gcttgcgcgg ctaacagaac atctttgacg
     2281 caattttcat tttttagcgt acgagcaatg tcagcggagg atgcccgcaa cggggccgga
     2341 ataatattca cactgcacgc cgcgttaagc tgtccgatac ccgtcatata agaaccgacg
     2401 ccaccggaga gcgtgaccag gcgaatttgc tgtgacgaaa taaaaccact taagcgttgc
     2461 agcgtattca tggttgcctc gccaaaacca atcgccagca tctgttgtgg ttgaagtaaa
     2521 ctcatcaaca tatgcgccgc gcctatccct agtcgcccgc cgacatcagc atccgcaagc
     2581 cctgggatca cccggacatg ttgcagcgaa aactgacgac gtaattgtgt ttcatattcc
     2641 agacagcctt caaagcgaga attaatctgt acgcgaataa tgccggattg atgccctttc
     2701 tccagtaatc gcgacacttt caaacgggtc aggccgagac gatcgctgat ctcactctgg
     2761 gtcagcccgt cgtgatagta aaaccacgcg atccgcgcga cctgttcttc ttcacacatt
     2821 ccctgttctg aaattaccga atcgttgatt gtcataattc attcttcact ttgaacatat
     2881 ttaaatcttt aatgcaattg ttcagttctt gcgcatttat atctgtgatg gcagccacag
     2941 tttgactcta cgagcatgaa caaacgcaac cgtgtaaatc aaaatagcat aaattgtgat
     3001 ctattcgtcg gaaatatgtg caatgtccac ctaaggttat gaacaaatta aaagcagaaa
     3061 tacatttgtt caaaactcac ctgcaaaact gaacggggga aatatgcaaa cgagtgatac
     3121 ccgcgcgtta ccgcttcttt gcgcccgctc ggtttataaa cagtattcag gggtcaatgt
     3181 cctgaaaggc atcgatttta cgttgcatca gggggaggtc cacgccctgc tcggcggcaa
     3241 tggtgccggt aaatcgacgt taatgaagat tattgccggt attacccctg ctgatagcgg
     3301 tacgctggag attgggggca acaactacgc cagattaacg ccagttcatg ctcatcagct
     3361 gggcatttat ctcgttcccc aggaaccgct gcttttccca agcctgtcga taaaagaaaa
     3421 catcctgttt gggatggcaa aaaaacagct ctccatgcaa aaaatgaaga acttgctggc
     3481 ggcgctgggc tgccagtttg atctgcatag tctggcagga tcgctggatg tcgccgatcg
     3541 ccaaatggtg gaaatcctcc gcgggctgat gcgcgactcg cggattctga tcctcgatga
     3601 acctaccgcc tcgcttaccc ctgcggaaac cgaacgcttg tttagtcgct tgcaagagct
     3661 gcttgctact ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa ttcgccagat
     3721 tgccgatcga attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca aaaccagcga
     3781 actgtctacc aacgacatta ttcaggccat caccccagcg gtacgggaaa aatcgctctc
     3841 tgccagccaa aaattatggc tggagttacc tggtaaccgc ccacaacatg ctgccggaac
     3901 gccggtgctg acactggaaa atctgaccgg cgaaggtttc aggaatgtca gcctgacgct
     3961 caatgccgga gaaattctgg gcctggctgg gctggtgggg gccggacgca cagaactggc
     4021 cgagacgttc tatggtctgc gtactttgcg tggcggacgt attatgctga atggtaaaga
     4081 gatcaataaa ttatccactg gagaacgttt actgcgcggt ctggtttatc tgccggaaga
     4141 tcgccagtca tccggactga atctcgatgc ttcgctggcc tggaacgtct gcgcccttac
     4201 tcataacctt cgtggattct gggcgaaaac cgcgaaagat aatgccaccc tggaacgtta
     4261 tcgtcgggcg ctgaatatta aatttaacca accggaacaa gctgcacgga cattatccgg
     4321 tggcaaccag caaaaaatcc tcattgccaa atgcctggaa gcttcgccgc aagtattgat
     4381 tgtcgatgag ccgacgcgcg gcgtggatgt ctcggcccgt aatgatatct accagctgtt
     4441 gcgcagcatc gccgcacaaa atgtggctgt gctgcttatc tcctctgatc tggaagagat
     4501 cgaactgatg gcagatcgcg tgtatgtgat gcatcagggc gaaattgccc actctgcact
     4561 gaccgggcgc gatattaatg tcgagactat tatgcgcgtt gccttcggcg atagtcagcg
     4621 tcaggaggcg tcatgctgaa gtttattcag aacaaccgtg aaatcacggc actgctggcg
     4681 gtggtgctgc tgtttgtatt acccggtttt ctcgaccgcc agtatttaag tgtgcaaacg
     4741 ctgaccatgg tttatagcag cgcgcaaatc ctgatcctgc tggcaatggg cgcgacgctg
     4801 gtaatgctta cgcgcaatat tgatgtttca gtgggttcga ttaccggaat gtgcgcggtg
     4861 ctgttgggga tgttactgaa cgcaggatat tcactacctg ttgcctgtgt cgcgacttta
     4921 ctgcttggtt tgctcgcggg atttttcaac ggtgtcctgg tcgcgtggct aaagatccct
     4981 gccattgttg ccacccttgg cacgttaggg ttgtacagag gcatcatgtt gctgtggact
     5041 ggcggtaaat ggattgaagg gttacccgcc gaactgaaac agctctccgc accgctgctg
     5101 tttggcgttt ccgcgattgg ttggttgacg ataattctgg tggcatttat ggcctggctg
     5161 ctggcaaaga cggcgtttgg acgcagtttt tatgccacgg gcgataattt acagggcgct
     5221 cgtcaactgg gtgttcgtac ggaagccatt cgcattgtgg cattttcgtt gaacggctgc
     5281 atggcggcac tggcgggaat tgtgtttgct tcgcagattg gttttatccc caaccagacc
     5341 ggtaccgggc tggagatgaa agcaattgca gcctgcgtgc tgggcggcat tagtttgctc
     5401 ggcggttccg gtgcgatcat cggtgcggta ctcggcgcat ggttcctgac gcagatcgat
     5461 agcgtactgg tgctgttgcg cattccggca tggtggaacg attttatcgc gggcctcgtg
     5521 ctgctggcgg tgctggtgtt tgatggacgc ctgcgttgtg cgctggaacg taatctacgg
     5581 cggcaaaaat atgcccgctt tatgacgcca ccgccatccg ttaaacccgc ttcgtcaggt
     5641 aaaaaacggg aggccgcata atgcgtattc gctacggttg ggaactggct cttgccgcac
     5701 tgctcgttat tgagattgtc gcatttggtg caattaaccc gcgaatgtta gatctcaata
     5761 tgttgctgtt cagcaccagt gactttatct gcattggcat tgtcgcccta ccgctgacga
     5821 tggtgattgt cagtggcggg atcgatattt cgtttggttc gaccatcggc ctctgcgcca
     5881 ttgcattggg cgtactgttt caaagtggtg tgccgatgcc gctggcgatt ctcctgacct
     5941 tactgctcgg cgcattgtgc gggctgatca acgccggatt aattatctat accaaagtta
     6001 acccgctggt gattacgctt ggcacgctgt atctgtttgc cggaagcgcc ctactgcttt
     6061 ccgggatggc cggagcgacg gggtacgaag gtattggtgg attcccgatg gcgtttacag
     6121 atttcgctaa cctggatgtg ctgggactcc ccgttccgct gattatcttc ctgatatgtc
     6181 tcctcgtttt ctggctctgg ctgcataaaa cccatgccgg acgtaatgtg tttttgattg
     6241 ggcaaagccc gcgcgtggcg ctttatagcg cgattccagt taaccgcacc ttatgtgcgc
     6301 tctatgccat gacggggctg gcgtctgcgg tcgccgctgt gctgctggtg tcgtattttg
     6361 gttcagcacg ttccgatctc ggtgcgtcgt ttctgatgcc cgccatcacc gccgtggtgc
     6421 ttggcggtgc caatatttat ggtggttccg gttccattat cggcaccgcc attgcggttt
     6481 tattagtggg atatttgcaa caaggtttgc aaatggcagg agtgccaaat caggtgtcca
     6541 gcgccctttc cggtgcgcta cttatcgtcg ttgtcgtagg tcgttccgtt agcctgcatc
     6601 gccagcaaat taaagagtgg ctggcgcgtc gggccaataa cccattgcca taaaggatat
     6661 cttcatgaca cttcatcgct ttaagaaaat cgccttactt agcgctcttg gcattgccgc
     6721 aatctctatg aatgtgcagg ccgcagagcg tattgcattt attcccaaac tggttggcgt
     6781 gggatttttt accagcggtg gcaacggcgc acaacaagcg ggtaaagagc tgagcgttga
     6841 tgtgacctac gacgggccga cagaacccag tgtttctggt caggtacagt tgattaataa
     6901 cttcgtcaat caaggttata acgccattat cgtttctgcg gtttcgcctg atggcttgtg
     6961 tccggcactg aaacgcgcca tgcaacgtgg tgtgagagtg ctgacctggg actctgatac
     7021 taaaccggag tgccgctctt actacattaa tcagggaacg cccgcccagt tgggaggtat
     7081 gttggtggat atggcggcgc gtcaggtgaa taaagacaaa gccaaagtcg cgtttttcta
     7141 ctcaagcccc accgttacgg accaaaacca gtgggtgaaa gaagcgaaag cgaaaatcgc
     7201 caaagagcat cctggctggg aaattgtcac tacgcagttt ggctataacg atgccactaa
     7261 atcattacaa accgcagaag gaatattaaa agcgtatagc gatctcgacg ccattatcgc
     7321 ccccgacgcc aacgccctgc ccgctgccgc acaagccgca gaaaacctga aaaatgacaa
     7381 agttgcgatt gtcggattca gtacgccaaa cgtgatgcgt ccatatgtgg aacgcggcac
     7441 ggtgaaagaa tttggcctgt gggatgtggt tcagcaaggc aaaatttcag tgtatgtcgc
     7501 ggatgcatta ttgaaaaaag gatcaatgaa aacgggcgac aagctggata tccagggcgt
     7561 aggtcaggtg gaagtctcgc caaacagcgt tcagggctat gactacgaag cggatggtaa
     7621 tggcatcgta ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg gcaaatacga
     7681 tttctgatgt gcattactta accggagtaa gttatggcag atttagacga tattaaagat
     7741 ggtaaagatt ttcgtaccga tcaaccgcaa caaaatatcc cttttatcct gaaaggttgc
     7801 ggtgcgctgg attggggaat gcagtcacgc ttatcgcgga tatttaatcc gaaaacgggt
     7861 aaaaccgtga tgctggcctt tgaccatggt tattttcagg ggccgactac cggacttgaa
     7921 cgcattgata taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc
     7981 ggcattttgc gcagcgtagt tcccccagcg accaataagc cggtggtact gcgggcgtca
     8041 ggtgcgaact ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat
     8101 gccgtgcgcc tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa
     8161 catcagtcga tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg
     8221 accatggccg tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc
     8281 gcgactcgaa tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa
     8341 ggttttgaac ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa
     8401 ttaccggagc gcgaggcgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt
     8461 gtggatatgg ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta
     8521 caggcggtgg ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa
     8581 aaacagtaac tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg
     8641 ttcatgaaga caagattgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg
     8701 tacaggaaga aggcaatttg cgcttcgatg tcttacagga cccagaagtg aattcgcgct
     8761 tttatatcta cgaagcctat aaagatgaag acgcagtggc attccataaa accacgcccc
     8821 actacaaaac ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc
     8881 tgttcaatgg gttgatgccg tgaggcgaat tcatcagttt tatctacaat tggggtaacg
     8941 cgctgacggg agtaaataaa tgtctgactg gaacccctct ttatatctgc actttgccgc
     9001 tgaacgatcg cgtccggcgg tggagctgct tgccagagta ccgctggaga atatcgaata
     9061 cgttgccgat cttggctgtg gcccaggtaa cagcaccgcc cttctaaacc aacgttggcc
     9121 tgctgccagg ataacaggca tcgactcgtc tccggcgatg attgctgaag cgcgcagtgc
     9181 tttgccagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg aacaggcact
     9241 cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg aattgtttcc
     9301 tcatctggtt tctttactta atccgcaggg cgtgctggca gtacagatgc cagataactg
     9361 gctggagccg acccatgtgc tcatgcgcga agttgcctgg gaacaaaact acccggatcg
     9421 cgggcgtgag ccgttggctg gcgttcatgc ttactacgat attttgagcg aagccggatg
     9481 tgaggtcgat atctggcgaa ccacctacta tcaccagatg ccgtcgcacc aggcgattat
     9541 cgattgggtg accgccactg gattacgtcc gtggttacag gatctgaccg agagcgaaca
     9601 gcagcttttt cttacgcgct accatcagat gctggaagag cagtatccac tgcaagagaa
     9661 cgaacagata ctgctggcat ttccgcgtct gtttattgtt gcccgccgta cggagtaatt
     9721 atcacgtcag ctggtaatga cgatcgggaa gaatttttgc tggaatttct gcttcatcgt
     9781 tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc atccagcggt aaatcattgt
     9841 tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa ataaaagtgt
     9901 aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc agcgcgaacg
     9961 gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag gcaaagggaa
    10021 ttggcgtgta ggcaatgcgc tcacagcctg ccagaaccgc tgaaatatca ttaagccgat
    10081 cgttgaggct aataaacagg atatctgaaa gctgtccatt gcggcgctga accgccaacc
    10141 attctcccat tattaacaag atacggttag ctggagagtt cgaagccagt acacgctgaa
    10201 gatcttcagt cttgagataa tgagccagca cttccgcctg tggctgtttg cgtaatgtca
    10261 tgcgtaaaca gtgggcgaag gcgatttgca gccgggcaaa ctcccttaca cttgccgaat
    10321 ccggcaatgt cgtttttacc tcgcgcagta acgaccgtga ggcaatcatc aactgccccc
    10381 aaagttttcg cgcttcaacg taacgggcgt acccggcatt attacgaaaa ccaagaaaaa
    10441 tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg cccagatgcg
    10501 tgtaccaggg cagcatgaat ataacagcga tagaaaagag aaaattgagt agtaagcgcg
    10561 aggatatctt ggataatact gagccgtgcc agacaaaaat acggcgcagc cagtgttgtt
    10621 gtggacgaac aatcatggtt atcttcaggc gtggaaaagt cgccctatta aacgtgatta
    10681 cgatcacgtt ctcaagacgc tacttacaaa ttacctacaa gcttacaaca ttaacaggcg
    10741 ctgcatgtgg cacctgttaa tgataaaggc acataggatg ttgtaactaa tatagtgaaa
    10801 taagaaaccc ggtcgaaacc gggttcagaa gtaacggtgt tattagcaca acggacgtac
    10861 agcttcgcgc atcccttttt caagaatcgc atccaggtca ttagcaacct gctcgaccag
    10921 accaggcact tgcgtcaggt cctgctccca gtggtctttt tctgccaaaa caatcgctac
    10981 cagttcctgg gtgccgataa cacggtcacg atgctggctc cagagttgct gataacgttc
    11041 cagccagtgt gcatcgtcct gtaccggata tgtttctccg ttacgctcac cgcgatagaa
    11101 ggcaatcaat gccgctaatg cgaaagtaag gcgcgccgga agtgtgcctt ttgccttctg
    11161 ccctgccagc agctgcggca ggatgcgggt gcggaacttg gtcataccgt tgagcgcgat
    11221 agacagcagc tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg cactggcgaa
    11281 agattccagt tcatcacgag gcaaatccag tactaccgga ataatttctt cgtagatggc
    11341 tttttcaacg aatgcgcaaa tttcagcatc gttcatcgct tcacctacgg tatccagccc
    11401 tgcctggaac gccaccggca ccagcgcggt gtgcgcaccg ttgaggattg ccactttgcg
    11461 ttctttatac ggtttaatat cgtcaacaat cagcacgttg agcggatatt tgtccaggcg
    11521 cagctcagtc gctaaggatt tcggcccctg aatcacaaac aggtaaaagt gttcagcggt
    11581 gtcgaggaaa ccatcgtgat aacccagttc ttcttccagt ttagccactt catcgcgcgg
    11641 ataaccggta acgatacggt ccaccagcgt agaacagaag ctgttagcct gatccagcca
    11701 ttgaataaat gcttctggca gtgcccactc ttgcgcatag cgcagcacca gttcacgcaa
    11761 cgcgtcgcca ttgtagtcaa tcaactcaca cggaatgatg atccaacctt tatccagcgc
    11821 accgttgaaa tggctaaagc gttcgaacag cagacgggtc agttttgccg gatagcttac
    11881 cgctggtgcg tcatcgaatt tatcgcccgc atggtagctg atccccgctt cggtggtgtt
    11941 agagaaaaca aagcgcattt ccgggttgtg cgccagtttc aggaattcat cgtattcact
    12001 gtagacgctg atttcacgat taaccgagcg aatcagacgc gcgtcgctga ccgcttcccc
    12061 cttctcattc aggccacgga taatggtggt gtacagacca tcctgcgtgc tcagtgacgg
    12121 cgggaatgaa gtttcaatcg gacgaacaac gaccacgcca gaattcagat cggtgtgctc
    12181 attcaggaga tcgatttgcc agtcaacaaa ggcgcgcagg aagttacctt caccaaactg
    12241 aatgatacgt tctggatact gtgcaccggg aaaatcgcga cgatttagtg ttttcacaat
    12301 gggttccctt ctgattagtc atacaacctg tttgaattgg tacgacaggt tagcaaactt
    12361 taatacgccg aacccctgtt ttgatcaact cctgatgatt aatgagcagt tttatgagaa
    12421 aagtgtggcg cggatcatgg tttaatcgag gaaatacgcc ttttcctgga tcataaagtg
    12481 gtagaacaca ttgcattcaa atcgcgcgga atgaataaag atgtcagaca acttcctcac
    12541 cgtaacgcat agtgctggta cggttgcgcc catctttctt cgagcgatac agacacgtat
    12601 cggcttcgac catcaattta ttaaaatcat cggtcagcgt tcggtacgat gcgcgaccac
    12661 taccgacgcc aatacttacc gtgagataaa gcgttttttg ttgccaggtg aatggttgca
    12721 gttcaacgcc tttacggatt ttttccgcca ttagcagacc atcgacagga ttcaccgacg
    12781 gcactgcaac agcaaattct tcgccgccca tgcgcgccac cagcccctta tcaccgacaa
    12841 tcttctgaat atgccgggca aacacgctta acactttatc gccacattca tgtccatagt
    12901 tatcgttaat gcttttgaaa tagtcgatat caagcagcat gacagtcaga tgttgtgtct
    12961 gtttcagcga tggacttttc agcgcctcat aaagaccgga ccgtgagtaa acctgagtca
    13021 gaaaatcaaa gtcggctcgc agcgcaactt gcttcattag cgaattgatc gctgccacgc
    13081 taaaagaaac cataattggg catatcgcca tcgtggcaat accgagacgt gcggagaaca
    13141 tttgcggaat ggagaacggc gaaccgaccg agatatcaat caccgaattt gccaccagta
    13201 cgatttccac cgcaccggtg acaaaggtca acagacatgt tacctgcggc gtatagcgca
    13261 ctgcacacca gattaatgca ggcagcggga acgccagact ccccgctccg ccaatgacta
    13321 ccgaggcaat aaccgacaca ataagcgcga tggcgggcat catctgttct gctttaaagc
    13381 gcggcagcac tccaggaata gccaacgtca gcatacaagg cacgatcaaa acgcccgttg
    13441 agaattgctc actgaaccag tccgcaagca aaggccagaa atccagacta tcaatactga
    13501 ccgaaccaat cgccccgaca atagcgcata ataatgcggc aatcagacag taattaaata
    13561 gccgtaaggc acttactggc tcatacttat ttttccccag acgcttatcc cgcacgacca
    13621 gtaaggcgac ggtaacaata aacatcatat tggagaaatt gataaccagt gaaaccagtc
    13681 cccattcggt ggttatggca tcataaacca gcatcgccac ataactgatc gcatagtaat
    13741 gcagtcgatt aagccagaca tagcgggcaa acacccccgc cattacgcca ttcagcggcc
    13801 agaaaagaga aagttcgtga accagccgta actctgcacc aataaaatag aacagcgtgg
    13861 ttagcgcaaa aatagcgatg ctattacgta gcagatgacc ttcctggaac aaacggaaag
    13921 ttgaaatggg gtgtacatgc attaattatc tgtccataca attgcgggtg gcaattatct
    13981 caacgtaata agtaaattca cataaaggat agcatgttcg ctaacagcgg cggcaaataa
    14041 aggtaccctg ggaatgctat tttatcgttt tccgcaattc tcatggctga ttcatctgta
    14101 caaccagcca gtgatagatt accgccacga tataacgttg ttgttcttcg cttaacgaaa
    14161 caccctgcgg aatgttatgc cacttagcca gacaaccgcg acagcaggtc gcggtggcgt
    14221 gctgagcgat aaacaccgga tgcccgcgca tgggggtttg cttaccgtca ttagccggta
    14281 atgccggagc aagacgttta gcgacaaaat ccgccgcatg ttgttcgatg actggcgcgc
    14341 ctttctccag gcaatactga cgctctttca tgcccagacg aaagcgagag cgaaattttg
    14401 aacgggataa acgcgcgaag agtggatcga gagactgcat taataaaccg aacgccctaa
    14461 ctgtttcgtc aattgttcaa ggacggcgat acccgcaagc gagttacctg catcatccag
    14521 ttccggactc cagactgcga tggccatttc atgcggaaca atcgccacaa taccgccacc
    14581 aacgccagat ttcgccggta gccccacccg ccaggcaaac tcccccgcgt tctggtacat
    14641 accactggtc gccatcagcg cgttaatttg ccgcgcctgc attggcgtca ccacaggttc
    14701 atcaatatga atagctttcc cctgattagc cagaaagaca aacgtccggg ccagctctac
    14761 acagctcatt ttcagagcgc agtaatgaaa gtagttttgc agaacggttg tcacatcatg
    14821 atggaaattg ccaaacgact tcatcagcca ggcgatagcc gcatttcgcg cggaatgttc
    14881 aaattcggaa cgcgctacca ccgtatcgta tgaaatatca gacacaccgc ttaagccgcg
    14941 cacgacttcc agcatacgtt gccgtggtgc gcttaatcgc ccttgcaaca tatcgcaaac
    15001 caccagcgca ccagcattaa taaacggatt acgcggaata ccctgctcca tttccagttg
    15061 cactaaagaa ttgaacggtg atccagacgg gtctttgccg acgcgttgcc agatttcctc
    15121 ttcggagtaa tgacgcatgg cgacaacgag actcagcact ttggaaatag actgaatgga
    15181 aaaacgttct tgcgcgtctc cggcctgaaa aagctgtccg tcaacggtac agatagcaat
    15241 gcccaatcgg gaaccgtcta ctgtagccag cgccggaata taatccgcga ctttaccctg
    15301 accaatgagc ggccgcactt gccgcaagat gtttcctaaa attgcattat ccatggcgac
    15361 tgccactttc tactcctgga ccgcaggtct gaaaagacct gcgagtatat cagagctgaa
    15421 tatgtcgcgt cagatccggt ctttccacac cgtctggata ttacagaatt cgtgtaagcc
    15481 gaaatgggaa agctcacgac caaagccact ctttttcacg ccaccaaagg ccactcgcgc
    15541 gtcgctggca caataaccat tgataaacac cccaccgcat tccagacgtg ccgccatctg
    15601 tctggcctgt gtttcgtcgg tggtaaaaat ggtcgctgaa aggccgaact cactatcatt
    15661 agccagttcc agcgcatgtt ctgcatcttt cgcaacggtg attgccgcaa cagggccaaa
    15721 catttcttcc cgaaacgcgg tcatttctgg ggtaacattc gccagaaccg ttggcggata
    15781 gtagttacct gccccagcca tcttttcccc gcccagtaac aaacgcgcac cctgcgccag
    15841 ggttttctcc acctgatgat gcagctcatc acgtaaatca aaacgagcca ttggtccgag
    15901 agcgttctct tcatcacggg gatcgcccat tttcaaggct gccgcagctg ccacaaaacg
    15961 ttcggtaaat gcagaagcaa ttccctcttc gataataaag cgttttgccg ctgcacaaac
    16021 ctgtccggta ttctgataac gtccggctac cgccgcttta accgccagtt ccagatcggc
    16081 atcgttaagc acaataaatg gatccgaacc gcccagttcc agtacgcatt ttttcagtgc
    16141 cgctccagcc tgtgcgccaa tagccgctcc cgcacgaaca cttccggtca ccgtgacagc
    16201 agcaatgcgc gaatctttaa tcatttgact gacaccgtcg ttgtcggcat tcagccagcc
    16261 atatacgcct tgcgggattc ccgcatcttt aaacacctgg gcaatgagct gagcacagcc
    16321 catcacattc ggcgcatgtt taagtaagta gccgttacct gcaagaatga tgggaaccgc
    16381 gccacgcatc acctgccata acggaaagtt ccacggcata atcgccagaa tcgtccccaa
    16441 cggtcgatac tcaataactg cctgctgatt ttccaccagc gtaggttccg ccttcagcat
    16501 tgccggacca tgttctgcat accagtcaca caaattcgcc gatttcgcca cttcagcgcg
    16561 cgcctgattg attggtttgc ccatttcacg ggtgatcatt tgcgccattt cttcgctacg
    16621 ggcgcgcaga gccttaccga taccacgcag tttttcagca cgataatcta tatttgtctc
    16681 gcgccagtcg cgaaagcctg ctgccgccag ctgaagtgcg ttttcgatat cgttagcgcc
    16741 agcccaaggc agcacagaaa gttgttcacc cgtggcagga tttattgaaa ttgcatgagt
    16801 tgccggagta atggtcatcg gggtatctcc tttatgagtc atggtatgaa gatacgcaga
    16861 tttactcttg ctttaaaatg aataatatta agccacttat tcacgaatcg agaatgttat
    16921 ggatctgacc caactggaga tgttcaacgc ggttgccgaa gctggcagca ttacccaggc
    16981 tgcagcaaaa gtgcatcgcg taccgtcgaa tctcactacc cgtctacgcc agctggaaac
    17041 agaactgggg gtagatctgt ttattcgcga gaatcagcgt ttacgtctct ctcctgccgg
    17101 gcataacttt ttacgctata gccaacaaat tctcgcgtta gtggatgaag cgcggagcgt
    17161 tgtcgctggc gatgagccgc aaggtttatt ttctcttggt tcgctggaaa gcaccgcagc
    17221 agtgcgcatt ccggccacgc tggcggaatt caaccatcgt tatcccaaaa ttcagttttc
    17281 actttccacc ggcccttccg gcactatgct ggaaggagtg ctggagggaa aactgaatgc
    17341 ggcatttatt gatgggccca ttaaccatac tgccattgac gggataccgg tataccgcga
    17401 ggagctgatg atcgtcacgc cacaaggtca tgcgccagta atccgcgcca gtcaggttaa
    17461 cggcagtaac atttatgcct tccgcgccaa ttgttcgtat cgtcgccact tcgagagctg
    17521 gtttcatgct gacggtgccg ctccgggaac tattcacgag atggagtcgt atcacgggat
    17581 gttggcctgt gtggtcgcag gagcaggcat tgcgcttatt ccacgctcta tgctggaaag
    17641 tatgccgggg catcaccagg ttgaagcgtg gccgttagct gagcaatggc gttggttaac
    17701 aacctggctg gtctggcgtc gcggtgcgaa aacgcgtccg cttgaggcat ttattcaact
    17761 actggatgtg cctgactcgg caaaacaggg ttaccaatga gctatttttg atagttcttg
    17821 cagctcaaat gccctatata gtgatgttcc gatgacttat gactatatgg ggcaaatatg
    17881 gttacgccag taagcatcag taattacata tctcttcctg atgattttcc cgtacgtaat
    17941 attgcacctc aggtaaaaga agttttaaag gactttattg atgcgctaag tacaataata
    18001 tgtgatgaag agtggcgcac gtctttaaac atcaactcag ccacaaaaaa gatatttaat
    18061 aaccttgata acttatcata tattcagagg acttctttt
//
LOCUS       DAZFRN010000095        17940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFRN010000095 DAZFRN010000000
VERSION     DAZFRN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.095"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..502
                     /gene="ybeT"
                     /locus_tag="ACHKUB_003164"
     CDS             <1..502
                     /gene="ybeT"
                     /locus_tag="ACHKUB_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6656143.1"
                     /translation="CISQPKKTTLRDKAMVNYAFDYLRSPGSLPFTTAATELSAIHGH
                     STSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDT
                     RKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLL
                     AGLPAH"
     gene            complement(512..1939)
                     /gene="djlB"
                     /locus_tag="ACHKUB_003165"
     CDS             complement(512..1939)
                     /gene="djlB"
                     /locus_tag="ACHKUB_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO6656144.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1936..2643)
                     /gene="ybeR"
                     /locus_tag="ACHKUB_003166"
     CDS             complement(1936..2643)
                     /gene="ybeR"
                     /locus_tag="ACHKUB_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6656145.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRNYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2807..3784
                     /gene="ybeQ"
                     /locus_tag="ACHKUB_003167"
     CDS             2807..3784
                     /gene="ybeQ"
                     /locus_tag="ACHKUB_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO6656146.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIREIEDLL"
     gene            complement(3854..4336)
                     /gene="ybeL"
                     /locus_tag="ACHKUB_003168"
     CDS             complement(3854..4336)
                     /gene="ybeL"
                     /locus_tag="ACHKUB_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO6656147.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4571..7153
                     /gene="leuS"
                     /locus_tag="ACHKUB_003169"
     CDS             4571..7153
                     /gene="leuS"
                     /locus_tag="ACHKUB_003169"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO6656148.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7168..7749
                     /gene="lptE"
                     /locus_tag="ACHKUB_003170"
     CDS             7168..7749
                     /gene="lptE"
                     /locus_tag="ACHKUB_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO6656149.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7749..8780
                     /gene="holA"
                     /locus_tag="ACHKUB_003171"
     CDS             7749..8780
                     /gene="holA"
                     /locus_tag="ACHKUB_003171"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO6656150.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8782..9423
                     /gene="nadD"
                     /locus_tag="ACHKUB_003172"
     CDS             8782..9423
                     /gene="nadD"
                     /locus_tag="ACHKUB_003172"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO6656151.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9447..10058
                     /gene="cobC"
                     /locus_tag="ACHKUB_003173"
     CDS             9447..10058
                     /gene="cobC"
                     /locus_tag="ACHKUB_003173"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO6656152.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10318..10635
                     /gene="rsfS"
                     /locus_tag="ACHKUB_003174"
     CDS             10318..10635
                     /gene="rsfS"
                     /locus_tag="ACHKUB_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO6656153.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10639..11106
                     /gene="rlmH"
                     /locus_tag="ACHKUB_003175"
     CDS             10639..11106
                     /gene="rlmH"
                     /locus_tag="ACHKUB_003175"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO6656154.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11137..13038
                     /gene="mrdA"
                     /locus_tag="ACHKUB_003176"
     CDS             11137..13038
                     /gene="mrdA"
                     /locus_tag="ACHKUB_003176"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO6656155.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13041..14153
                     /gene="mrdB"
                     /locus_tag="ACHKUB_003177"
                     /gene_synonym="rodA"
     CDS             13041..14153
                     /gene="mrdB"
                     /locus_tag="ACHKUB_003177"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO6656156.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14164..15252
                     /gene="rlpA"
                     /locus_tag="ACHKUB_003178"
     CDS             14164..15252
                     /gene="rlpA"
                     /locus_tag="ACHKUB_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO6656157.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15392..16603
                     /gene="dacA"
                     /locus_tag="ACHKUB_003179"
     CDS             15392..16603
                     /gene="dacA"
                     /locus_tag="ACHKUB_003179"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO6656158.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAMMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16714..16977
                     /gene="ybeD"
                     /locus_tag="ACHKUB_003180"
     CDS             16714..16977
                     /gene="ybeD"
                     /locus_tag="ACHKUB_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO6656159.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17078..17719
                     /gene="lipB"
                     /locus_tag="ACHKUB_003181"
     CDS             17078..17719
                     /gene="lipB"
                     /locus_tag="ACHKUB_003181"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO6656160.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4534 a         4508 c         4572 g         4326 t
ORIGIN      
        1 ctgtattagc caacccaaga aaacgacgtt gcgtgataaa gccatggtga attatgcctt
       61 tgattattta agatcaccgg gcagtcttcc attcaccacg gcagccacgg agctttccgc
      121 gattcatggt cactcaacgt cgcaatatcg ccttggagaa ttttatcttc atggtagcga
      181 cggtaaacca ctggattata cacaggcgag atactggtat gagcaatcag cggaacagga
      241 aaatccacgc gcgcaaagta aactggggtg gatctacctc aaaggtctgg gggtcaaacc
      301 cgacacccgt aaagcaattc tctggtataa ggaagcagct gaacaagggt atgctcatgc
      361 tcaatatact ttaggtttga tctacagaaa tggctcaggt attaatgtta accattatga
      421 atctcaaaaa tggttaaaac tggccgccaa acaacattac aaaaatgcgg aaagattact
      481 tgccgggctt cccgcacatt aacgtcgact gtcacccgaa cagatggagt attttcccta
      541 tcaaaccagc aaagataaaa atatagaaaa taattttaat gatgcccaga cagcctggcc
      601 caccatcgtc aacgggcatt ggctgcggtt gcggctccgg ttcaacagtt tgcttctcgg
      661 taaaattcgc cgcctgaatt aataatcgac gaataattaa tgcttccagt ggacaacgat
      721 cttccgcatc aaggcggctc acaatcggca ttaatgattc ttctgtcggt tggctagcct
      781 gcactaattg tttggcaact aaatataatg gccataaggg atgttctgta tcacgcgtga
      841 gtgagatccg cggcttacgt cctttgtgca aaaccatctc gaccagtgaa cgtcccagcg
      901 cagagagttt ttcatcgctg agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc
      961 cggggacata aagtagcggt tttcccttat cgttaaccag atcacgcgct tcaatcatca
     1021 tcaccactaa tgggtaataa tcgacccgat gctgacggca ccattgcaac attaaagcat
     1081 ccgcctgggt ggagggatac tcgcgccagt agtcacaaag ttctgccagc aagctttcgc
     1141 cttctccaca atagacgcgt tgcgcgtatt cataataacg cgcatcttca ttatcaggtt
     1201 cagcttcccg ccacgaccgg gcgtaatcca gaagttcggg gatatccagg cgacctgcgc
     1261 tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc ggggatgatc actgcgcgat
     1321 gttgcgctaa atacaatgtc agccattctg gatgcgatga ccaaatccga gcgaacattt
     1381 ggtaaaaatc gatggtgtca ttttgcacgg caacgggcag atgcagaatg gtctggaagt
     1441 taaaaacatt ccccttcgca atggcatata aaaaggattc cagttcttct tcgtctattt
     1501 ccccgacgtt ttcctcctgc aaccaccgca atctttctgc taataacacc acgcactcga
     1561 aggataaatg ggcagtgttc atggcgattg tgcacagcga ccagcgtaat tcatcaatat
     1621 cttccatcga gcaataattt aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac
     1681 gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag aatttcatgt tctgctacct
     1741 catattcttc tggttgccaa acagatttag caggcgactg cgcaatccgg agcgcctcct
     1801 catacgcctg acgaagttgt ttaaaaccct gcggatcggt ttctggatga aaggacggta
     1861 acagcgccag ataagcgcgg cggataatat cgacgtcagt cgtttcctct atttcgagaa
     1921 tcttccagca atttttcata ggtcgctcat atccttaagc gtttccgggc atgcagaata
     1981 gtaagccaat ggttgccagg acaaggtagg gtaatacgca tcatcactgg caaataattg
     2041 ataaaacata tcattgccgg aatcgtcgta ttctttacgc gctaagaatt cgcgtaataa
     2101 ctgcagatga tcatcttcgg gagactgcca atattgccga ccaaaggtat aaccggcaaa
     2161 gtattgccgc cagttacgat aataacgtag agcccgtaga tgaatgcgcg actggatcca
     2221 tagactttct tcttcgctca accagttatt aagtacgccc attcggctta aaaaacccat
     2281 ccggacataa tcccaggctt taattcctcc ctcaccacag cattcggcgg tgctggcgac
     2341 aaattgcgca taagcctgac cttgctcatt tagttcagca agatagttac gccattcaca
     2401 tggagaataa cgaaaccagc ggtgataaaa ccctgccaac cgagcggcat gaccgttctc
     2461 gatcattcgg tgaattgtgc gcagcaggtc atcacggtca tcaattcccc atgaggaaga
     2521 aaggtcaaaa aagcatgtac ctaaatacat tttgggtgat gaatgcgaag gtaaacagca
     2581 ttcattgcgt atttccatcg gagtggaaag tgcaaacaat attttttgcg actccatgtc
     2641 catataattt atccttaaat attcattaaa tgaatattag ccgcattgcg aggctcgttt
     2701 atttatactt ttctgacgcg acataatacg cataaaataa ggcttacgca aaaatggaac
     2761 acataaaaat ataatatgtt ttatctgcaa ccataaggaa atgataatga ttttcacgtc
     2821 aagttgctgc gataatttat caatagatga gattatcgaa cgtgctgaaa aaggcgattg
     2881 cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg attctccgga
     2941 cgacgaaaaa gccttttact ggctgaagct ggccgccgag caaggtcatt gtgaagcaca
     3001 atattcctta ggacaaaagt atactgagga taaaagccgt cataaagata atgagcatgc
     3061 catcttttgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca acgcccttgg
     3121 ctggatactg gatcgtggag aagaccccaa ttataaagaa gcggttgtct ggtatcagat
     3181 agccgcggag agcggaatgt cttatgcgca aaataatctt gggtggatgt acagaaatgg
     3241 caacggagtc gcaaaagact atgcgctggc atttttctgg tacaaacaag ctgcattaca
     3301 aggccatagt gacgcgcaaa acaatctggc cgatctttat gaagacggaa aaggcgttgc
     3361 tcaaaacgag acactcgccg cattctggta tttgaaaagc gcacagcagg gtaatcggca
     3421 tgctcagttt caaattgcat gggattataa cgctggcgaa ggggtggacc aggactataa
     3481 gcaagcgatg tactggtatc tgaaggctgc cgctcagggg agcgtcggcg cttacgtcaa
     3541 catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg ctgcctttga
     3601 atggtttacg aaagccgctg aatgcaatga cgccactgct tggtataacc tggccattat
     3661 gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc tgtatcgtaa
     3721 agttcagtca tccggaacca gagatgtcag tcaagaaatt cgtgagattg aagatttact
     3781 gtagagataa aaacaagggc cggggaattt cattcggccc atattctctt tattgctgtg
     3841 cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcatga ccacatttcg
     3901 ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga
     3961 ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga ttgaggtcct
     4021 ggaaaacttc gcgccattca agctgcgttt tatcggtgat gtctgccagc tcctgccaca
     4081 agctttcttt aatcacccgc ataaagacgc tgtcagattc ttctttcagg ctctcttcat
     4141 agctcatggc gaactcttcc aggtcacgtc tgacagctcg cgtcagctca tcgacctcgg
     4201 ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga
     4261 tatcacgttc gccattgcgc aggcgttcgc tcagtgacgc aaccagttca cggtaatatt
     4321 gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat aatagacgtt
     4381 ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta tgaataatcc
     4441 gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg
     4501 ctatgcggat ctgaaaaacc acctcaacgc tacatttgta gccgtattga aaacaggacc
     4561 actggctgcc atgcaagagc aataccgccc ggaagagata gaatccaaag tacagcttca
     4621 ttgggatgag aagcgcacat ttgaagtaac cgaagacgag agcaaagaga agtattactg
     4681 cctgtctatg cttccctatc cttctggtcg actacacatg ggccacgtac gtaactacac
     4741 catcggtgac gtgatcgccc gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat
     4801 cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc
     4861 ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt
     4921 tggttatgac tggagccgcg agctggcaac ctgtacgccg gaatactacc gttgggaaca
     4981 gaaattcttc accgagctgt ataaaaaagg cctggtatat aagaagactt ctgcggtcaa
     5041 ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa gttatcgacg gctgctgctg
     5101 gcgctgcgat accaaagttg aacgtaaaga gatcccgcag tggtttatca aaatcactgc
     5161 ttacgctgac gagctgctca acgatctgga taaactggat cactggccag acaccgttaa
     5221 aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa
     5281 cgactatgac aacacgctga ccgtttacac tacccgcccg gacaccttta tgggttgtac
     5341 ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga
     5401 actggcggcc tttattgacg aatgccgtaa caccaaagtt gccgaagctg aaatggcgac
     5461 gatggagaaa aaaggcgtcg atactggctt taaagctgtt cacccattaa cgggcgaaga
     5521 aattcccgtt tgggcagcaa acttcgtatt gatggagtac ggcacgggcg cagttatggc
     5581 ggttccgggt cacgaccagc gcgactacga gtttgcctct aaatacggcc tgaacatcaa
     5641 accagttatc ctggctgctg acggctctga gccagatctt tctcagcaag ccctgactga
     5701 aaaaggcgtg ctgttcaact ctggcgaatt caacggtctt gaccatgaag cggccttcaa
     5761 cgccatcgcc gataaactga ctgcgatggg cgttggcgag cgtaaagtga actaccgcct
     5821 gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg ccgattccga tggtgacgct
     5881 ggaagacggt accgtaatgc cgaccccgga cgaccagctg ccggtgatcc tgccggaaga
     5941 tgtggtgatg gacggcatta ccagcccgat taaagcagat ccggagtggg caaaaactac
     6001 cgttaacggt atgccagcgc tgcgtgaaac cgacactttc gacaccttta tggagtcctc
     6061 ctggtactat gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg attccgaagc
     6121 ggctaactac tggctgccgg tggatatcta cattggtggt atcgaacacg ccattatgca
     6181 cctgctctac ttccgcttct tccacaaact gatgcgtgat gcaggcatgg tgaactctga
     6241 cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct tctactatgt
     6301 tggcgaaaac ggcgaacgta actgggtttc cccggttgat gctatcgttg aacgtgacga
     6361 gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat gaactggttt ataccggcat
     6421 gagcaaaatg tccaagtcga agaacaacgg tatcgacccg caggtgatgg ttgaacgtta
     6481 cggcgcggac accgttcgtc tgtttatgat gtttgcttct ccggctgata tgactctcga
     6541 atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt
     6601 ttacgagcac acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga
     6661 tcagaaagcg ctgcgtcgcg atgtgcataa aacgatcgct aaagtgaccg atgatatcgg
     6721 ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg gagctgatga acaaactggc
     6781 gaaagcacca accgatggcg agcaggatcg cgctctgatg caggaagcgc tgctggccgt
     6841 tgtccgtatg cttaacccgt tcaccccgca catctgcttc acgctgtggc aggaactgaa
     6901 aggcgaaggc gatatcgaca acgcgccgtg gccggttgct gacgaaaaag cgatggtgga
     6961 agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttcc
     7021 ggtggacgca acggaagaac aggttcgcga acgtgctggc caggaacatc tggtagcaaa
     7081 atatcttgat ggcgttaccg tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct
     7141 ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg caacattgtt gttatctctg
     7201 gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg ataccacgca ggttccttcc
     7261 actatgaagg tcatgatcct ggactcaggc gatccgaacg ggccattaag ccgtgcggtg
     7321 cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac
     7381 gttccatccc tgcgtttggg taaagtgagc atcgcgaaag ataccgcatc ggtattccgt
     7441 aacggtcaaa cagcagagta tcagatgatc atgacggtga atgcgaccgt gttgatcccc
     7501 ggccgtgata tctacccgat tagcgccaaa gtcttccgtt cgttctttga taacccgcaa
     7561 atggcgttag cgaaagataa cgaacaagac atgatcgtaa aagagatgta cgaccgtgct
     7621 gccgaacagc tgattcgtaa gctgccaagc atccgtgctg cggatattcg ttccgacgaa
     7681 gaacagacgt cgaccacaac ggatactccg gcaacgcctg cacgcgtctc caccacgctg
     7741 ggtaactgat gattcggttg tacccggaac aactccgcgc gcagctcaat gaagggctgc
     7801 gcgcggcgta tcttttactt ggtaacgatc ctctgttatt gcaggaaagc caggacgctg
     7861 ttcgtcaggt agctgcggca caaggattcg aagaacacca cactttttcc attgatccca
     7921 acactgactg gaatgcgatc ttttcgttat gccaggctat gagtctgttt gccagtcgac
     7981 aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc
     8041 tcacactcac cggacttctg catgacgacc tgctgttgat cgtccgcggt aataaattaa
     8101 gcaaagcgca agaaaatgcc gcctggttta ctgcgcttgc gaatcgcagc gtgcaggtga
     8161 cctgtcagac accggagcag gctcagcttc cccgctgggt tgctgcgcgc gcaaaacagc
     8221 tcaacttaga actggatgac gctgcaaatc aggtgctctg ctactgttat gaaggtaacc
     8281 tgctggcgct ggctcaggca ctggagcgtt tatcgctgct ctggccagac ggcaaattga
     8341 cattaccgcg cgttgaacag gcggtgaatg atgccgcgca tttcacccct tttcattggg
     8401 ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca tattcttcag caactgcgtc
     8461 tggaaggcag cgagccggtt attttgttgc gcacattaca acgtgaactg ttgttactgg
     8521 ttaacctgaa acgccagtct gcccatacgc cactgcgtgc gttgtttgat aagcatcggg
     8581 tatggcagaa ccgccggggc atgatgggcg aggcgttaaa tcgcttaagc cagccgcagt
     8641 tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa gattacggtc
     8701 agtcagtgtg ggcagagctg gaagggttat ctcttctgtt gtgccataaa cccctggcgg
     8761 acgtatttat cgacggttga tatgaaatct ttacaggctc tgtttggcgg cacctttgat
     8821 ccggtgcact atggtcatct taaacccgtg gaaacgctgg cgaatttgat tggtctgacg
     8881 cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc cccagccgga agcgaacagc
     8941 gtgcagcgta aacacatgct tgaactggcg attgccgaca agccattatt tactcttgat
     9001 gaacgcgagc taaagcgcaa tgccccctct tacactgcgc aaacactgaa agagtggcgg
     9061 caggaacaag ggccggacgt gccgctggcg tttattattg gtcaggattc attgctgacc
     9121 tttccgacct ggtacgaata cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg
     9181 cgtccaggtt acccacttga aatggcgcaa ccgcaatacc agcaatggct ggaagatcat
     9241 ttgacacata acccggaaga tcttcacctt cagcctgccg gtaaaattta tctggctgaa
     9301 acgccgtggt ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg
     9361 tgtgaggatt tattgccgga accggtactg acttacatta accaacaagg cttgtatcgc
     9421 tgatacctgt cgttacggag ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc
     9481 aagcgaatat cgatggtcta tacagcggtc atgcgcccac ccccctgacc gcgcgcggta
     9541 ttgagcaagc gcaaaatctg catacgctgc tacatggtgt ttcctttgat ctggttttat
     9601 gcagtgaact ggaacgggca cagcataccg cgcgactggt tctcagtgac cgccagctcc
     9661 ccgttcatat catccctgaa ctcaacgaaa tgttttttgg cgactgggag atgcgacatc
     9721 atcgcgacct catgcaagaa gatgccgaaa actatagcgc gtggtgcaat gactggcagc
     9781 atgcaatccc cacgaacggt gaaggatttc aggcattttc gcaacgtgtg gaacgcttta
     9841 tcgcaaggct tagtgaattt cagcactatc agaatatttt agtcgtcagc catcagggtg
     9901 tactgagtct gttaatcgcc cgtttaattg gcatgcctgc cgaggctatg tggcattttc
     9961 gcgttgacca ggggtgttgg agcgccattg atatcaacca aaaattcgcg acgctacgcg
    10021 tcctcaatag ccgtgccatc ggggtcgaga atgcatgact tttctgtttt tttacgggta
    10081 agccgcaacg accattgaca gacccgggca ggctgatatt ctccgcagcc agactttttt
    10141 cgccagacac gactttgtag aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg
    10201 ggtgggatga tagcccactt tcgaaagccg attcggcgac aattgtcccg aaatcgcctc
    10261 tggttcaggt atactgacag accattttta tctatttgat tcacccaggg ggaaaacttg
    10321 cagggtaaag cactccagga ttttgttatc gacaaaattg atgacctcaa aggtcaggac
    10381 atcatcgcct tagacgttca aggcaaatcc agcatcaccg actgcatgat catctgtacg
    10441 ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca
    10501 gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat
    10561 ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa
    10621 aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac
    10681 tgggtacaaa ccggttttac cgagtacctg cgtcgttttc cgaaagatat gcccttcgag
    10741 ctgattgaaa ttccggccgg aaaacgcggc aagaatgcgg acatcaagcg catactcgac
    10801 aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt
    10861 ccaggcaagc cctgggatac gccgcagtta gccgctgagc tggaacgctg gaagctggat
    10921 ggtcgcgacg tcagtctact gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg
    10981 gcggcggagc agagctggtc gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg
    11041 ctggtcgcag agagtctgta ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt
    11101 gagtgataag ggagctttga gtagaaaacg cagcggatga aactacagaa ctcttttcgc
    11161 gactatacgg cagagtccgc gctgtttgtg cgccgggcac tggtcgcctt tttggggatt
    11221 ttgctgctga ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt tcgctttacc
    11281 gactaccaga ctcgctctaa tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc
    11341 ggcattatct acgaccgtaa tggtatccct ctggccctca accgcactat ctaccagata
    11401 gaaatgatgc cagagaaagt cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg
    11461 gttgatctga ccgatgacga tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt
    11521 ttcacctcta ttccggtgaa aactaacctg accgaagtac aagtagctcg ctttgccgtc
    11581 aatcagtacc gttttccggg tgtcgaagtt aaaggctata aacgtcgtta ctatccttac
    11641 ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa
    11701 cgcctgaata atgacggcaa actggccaac tatgcggcaa cgcatgatat cggtaagctg
    11761 ggcattgagc gttactatga agacgtgctg cacggtcaga ccggttatga agaggttgaa
    11821 gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag taccaccgca agccggacac
    11881 gatatttacc tgacgctgga tctcaaactc cagcaatata ttgaaacgct gctggcgggt
    11941 agccgcgcag ctgtggttgt caccgatccg cgtacaggtg gggtgctggc gctggtttcc
    12001 acgcctagtt atgacccaaa cctgtttgtt gacggtatct ccagcaaaga ttattccgcc
    12061 ttgttgaacg acccgaatac accgctggtg aaccgcgcca cacagggggt ttatcctccc
    12121 gcgtctacag ttaaacccta tgtggcggtt tcggcattga gcgccggggt gatcacgcgc
    12181 aatacgacgc tgtttgaccc aggctggtgg caactgccag gttcggaaaa acgttatcgt
    12241 gactggaaaa aatggggcca cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg
    12301 gataccttct tctatcaggt ggcctacgat atggggatcg atcgcctctc cgaatggatg
    12361 ggtaaattcg gttatggtca ttacaccggt atcgacctgg cggaagaacg ttccggcaac
    12421 atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca gggtgacacc
    12481 attccggttg gtatcggtca gggttactgg acagcgaccc caatccagat gagtaaggca
    12541 ctgatgatcc tgattaatga cggtatcgtg aaggttcctc atttgctgat gagcaccgcc
    12601 gaagacggca aacaggtgcc atgggtacag ccgcatgaac cgcccgtcgg cgatattcat
    12661 tccggttact gggagctggc gaaagacggt atgtacggtg ttgctaaccg ccctaacggt
    12721 acggcgcata aatactttgc cagcgcaccg tacaaaattg cggcgaaatc cggtaccgct
    12781 caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc cgagcgttta
    12841 cgtgaccaca aactgatgac cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc
    12901 atgattctgg agaacggtgg tgcgggtccg gcggttggta cactgatgcg ccagatcctc
    12961 gaccacatta tgctgggtga taacaacacc gatctgcctg cggaaaatcc agcggttgcc
    13021 gcagcggagg accattaatc atgacggata atccgaataa aaaaacattc tgggataaag
    13081 tccatctcga tcccacaatg ctgctgatct tactggcatt gctggtttac agcgccctgg
    13141 ttatctggag cgccagcggt caggatattg gcatgatgga gcgcaaaatc ggccaaatcg
    13201 cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct
    13261 gggcccccta tctctatatc atctgtatta ttttgctggt ggcggtagat gctttcggtg
    13321 ccatctctaa aggtgctcaa cgctggctgg acctcggtat tgttcgtttt cagccgtcgg
    13381 aaattgccaa aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc
    13441 cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat atttatgccc acgctgctgg
    13501 tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac
    13561 tgttcctctc tggccttagc tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt
    13621 tcattccgat tctgtggttc ttcctgatgc atgattacca gcgccagcgc gtaatgatgc
    13681 tcctggaccc ggaatcagac ccactcggcg cgggctatca cattattcag tctaaaattg
    13741 ctattggctc cggcggatta cgcggcaaag gctggctgca cggcactcag tcacagcttg
    13801 aatttctccc cgaacgccat accgacttta tcttcgcggt actggcggaa gagctgggat
    13861 tagtgggcat tctgattctg ctcgctctct acattctgct gatcatgcgc gggctgtgga
    13921 tagccgccag agcgcaaacc acctttggtc gcgtcatggc tggtggctta atgctgatat
    13981 tattcgttta tgtcttcgta aatattggta tggtaagcgg tattctgccg gttgtagggg
    14041 ttccgctccc actggtcagt tatggaggat cggcgctaat tgtgctgatg gctgggttcg
    14101 ggattgtaat gtcaatccac acccacagga aaatgttgtc gaaaagcgtg taagaggtgc
    14161 gcaatgcgta agcagtggct cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca
    14221 agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc ctgcggtatg taacggcccc
    14281 atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag
    14341 gattaccagc gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg atttagccag
    14401 gcgggactgg cggcaatcta tgatgccgaa ccaggcagta acctgacggc ctctggcgaa
    14461 gctttcgatc cgacacagct gacggcggcc catccaacgc ttccgatccc cagctacgcc
    14521 agaatcacta acctggctaa cgggcgaatg atcgtggtgc gcattaatga tcgcggccct
    14581 tacggcaacg accgcgttat ttcgctttct cgcgcggcag ctgaccgtct taacacgtca
    14641 aacaacacca aagttcgtat cgatccgatt attgtcgccc aggatggttc gctttctggt
    14701 cctggtatgg cttgtaccac agtcgccaaa cagacttacg ccctgcctgc acctcccgat
    14761 ttaagcggtg gcgcgggggc aagttcagtg tctggcccgc agggtgacat tcttccggtc
    14821 agtaactcga cgctaaaaag cgaagatccg accggcgcgc cggtaaccag tagcggtttc
    14881 ctcggcgctc caacgacctt agcgcctggt gtactggaaa gcagcgaacc gacacctgca
    14941 ccacagcccg ttgttacagc tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca
    15001 ccgcaagccg cctcgcaaag cgccagcggc aactttatgg tgcaagtcgg ggccgttagc
    15061 gatcaggctc gtgcgcaaca gtaccaacag caactgggac agaagttcgg cgtccccggt
    15121 cgcgtaactc aaaatggcgc ggtctggcgt atccagcttg gcccattcgc cagtaaagcc
    15181 gaagccagta ccttgcagca acgtttgcaa accgaagccc aattacagtc atttattacc
    15241 accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa
    15301 agtcacgcgg aaagtcagat gcctgccggt agtgcatttg ctatagtagg gcactttttt
    15361 taattccatc acggatgtcg tagttcagac catgaatacc attttttccg ctcgtatcat
    15421 gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg cacatgccga
    15481 tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg agtcctacat
    15541 cctgattgac tataactccg gcaaagtgct cgccgaacag aacgcagatg tccgccgcga
    15601 tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa tgaaagccgg
    15661 taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca ccggtaaccc
    15721 ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc cggtttctca
    15781 gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgcgtcgcca tggccgattt
    15841 tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta acgccctggg
    15901 cctgaaaaac acccacttcc agacggtaca tggtctggat gctgatggtc agtacagctc
    15961 cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga atgaatactc
    16021 gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc gtaacggcct
    16081 gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg acaaagcagg
    16141 ttacaacctt gttgcttctg cgactgaagg ccagatgcgc ttgatttctg cgatgatggg
    16201 cggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct ggggcttccg
    16261 tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg aaccggtttg
    16321 gtttggtgat tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc tgaccattcc
    16381 acgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg aattgcatgc
    16441 gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg gcaaaacgat
    16501 cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct tcggcaaaat
    16561 cattgattac attaaattaa tgttccatca ctggtttggt taaaaattga acacttgaaa
    16621 gtgtaatttc cgtccccata tactaagcat cagtaaaaaa actcccgcct tctggcggga
    16681 gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa cgaactgctt
    16741 gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga gctggttgat
    16801 caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac ggtaaaacca
    16861 agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca tatcgagcag
    16921 gttgaaacac tgtatgaaga actgggcaaa atcgatattg tccgcatggt tctgtaactc
    16981 gcttctccgt tacccggctc ctggtcgggt aactcccctc tcctcgctgt gatatacttt
    17041 tcccccactt ttactcattc tccacggaga tgccgttttg tatcaggata aaattcttgt
    17101 ccgccagctc ggtcttcagc cttacgagcc aatctcccag gctatgcatg aattcaccga
    17161 tacccgcgat gatagtaccc ttgatgaaat ctggctggtc gagcactatc cggtattcac
    17221 ccaaggtcag gcaggaaaag cggagcacat tttaatgccg ggtgatattc cggtgatcca
    17281 gagcgatcgc ggtgggcagg tgacttatca cgggccggga caacaggtga tgtatgtgtt
    17341 gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc ttgagcaaac
    17401 agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg acgcgcctgg
    17461 tgtctatgtc ggggaaaaga aaatttgctc actgggttta cgaattcgac gcggttgttc
    17521 attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac gtattaatcc
    17581 ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg aagcgacgac
    17641 taataatatt gctccacgtt tactggaaaa tattttagcg ctactaaaca atccggactt
    17701 cgaatatatt accgcttaat tccatacctc aatggcccaa tttacactgg gtcattactc
    17761 ataatacctg ctaatgcttt acatctcctt cgcgtttgta tattttcttt atcacttgca
    17821 gaaatgcggt gccacgcctt gctccgtaac tcaccccatt gattaaccta ttgaaatagt
    17881 tgattatggt gagcaaaagc gttccgttcc aattagacat aagattattg taatttttta
//
LOCUS       DAZFRN010000096        17922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFRN010000096 DAZFRN010000000
VERSION     DAZFRN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.096"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            57..560
                     /locus_tag="ACHKUB_003182"
     CDS             57..560
                     /locus_tag="ACHKUB_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO6656161.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(627..2084)
                     /gene="nuoN"
                     /locus_tag="ACHKUB_003183"
     CDS             complement(627..2084)
                     /gene="nuoN"
                     /locus_tag="ACHKUB_003183"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO6656162.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2091..3620)
                     /gene="nuoM"
                     /locus_tag="ACHKUB_003184"
     CDS             complement(2091..3620)
                     /gene="nuoM"
                     /locus_tag="ACHKUB_003184"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO6656163.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3784..5625)
                     /gene="nuoL"
                     /locus_tag="ACHKUB_003185"
     CDS             complement(3784..5625)
                     /gene="nuoL"
                     /locus_tag="ACHKUB_003185"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO6656164.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5622..5924)
                     /gene="nuoK"
                     /locus_tag="ACHKUB_003186"
     CDS             complement(5622..5924)
                     /gene="nuoK"
                     /locus_tag="ACHKUB_003186"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO6656165.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5921..6475)
                     /gene="nuoJ"
                     /locus_tag="ACHKUB_003187"
     CDS             complement(5921..6475)
                     /gene="nuoJ"
                     /locus_tag="ACHKUB_003187"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO6656166.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6487..7029)
                     /gene="nuoI"
                     /locus_tag="ACHKUB_003188"
     CDS             complement(6487..7029)
                     /gene="nuoI"
                     /locus_tag="ACHKUB_003188"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO6656167.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7044..8021)
                     /gene="nuoH"
                     /locus_tag="ACHKUB_003189"
     CDS             complement(7044..8021)
                     /gene="nuoH"
                     /locus_tag="ACHKUB_003189"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO6656168.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8018..10744)
                     /gene="nuoG"
                     /locus_tag="ACHKUB_003190"
     CDS             complement(8018..10744)
                     /gene="nuoG"
                     /locus_tag="ACHKUB_003190"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO6656169.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10797..12134)
                     /gene="nuoF"
                     /locus_tag="ACHKUB_003191"
     CDS             complement(10797..12134)
                     /gene="nuoF"
                     /locus_tag="ACHKUB_003191"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO6656170.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12131..12631)
                     /gene="nuoE"
                     /locus_tag="ACHKUB_003192"
     CDS             complement(12131..12631)
                     /gene="nuoE"
                     /locus_tag="ACHKUB_003192"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO6656171.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12634..14436)
                     /gene="nuoC"
                     /locus_tag="ACHKUB_003193"
     CDS             complement(12634..14436)
                     /gene="nuoC"
                     /locus_tag="ACHKUB_003193"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO6656172.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14530..15192)
                     /gene="nuoB"
                     /locus_tag="ACHKUB_003194"
     CDS             complement(14530..15192)
                     /gene="nuoB"
                     /locus_tag="ACHKUB_003194"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO6656173.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15208..15651)
                     /gene="nuoA"
                     /locus_tag="ACHKUB_003195"
     CDS             complement(15208..15651)
                     /gene="nuoA"
                     /locus_tag="ACHKUB_003195"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO6656174.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16282..17220)
                     /gene="lrhA"
                     /locus_tag="ACHKUB_003196"
     CDS             complement(16282..17220)
                     /gene="lrhA"
                     /locus_tag="ACHKUB_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO6656175.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4483 a         4994 c         4613 g         3832 t
ORIGIN      
        1 attttccctc gtctgtttta agatgcgaaa cataaaattc aatggaggaa aaataaatgg
       61 gaatgattgg ttattttgca gaaattgact cagaaaagat taaccaactg cttgagtcta
      121 ctgaaaaacc tttgatggat aatatacatg acacgctttc aggattacgc cgactggata
      181 ttgataaacg ctgggatttt ttacactttg ggctgacagg cacctctgct tttgatcccg
      241 cgaagaatga tccgctgagt cgtgccgtgc ttggtgaaca cagcctcgaa gatggcatag
      301 atggcttcct gggattgacc tggaatcagg agttagccgc gacaatcgac cgactggaat
      361 cactggatcg aagtgagctg cgtaaacagt tttccattaa gcgactcaat gaaatggaga
      421 tttatccggg agtgaccttt tcagaagagt tagaggggca actctttgct tcgatcatgc
      481 ttgatatgga aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg aatcatgcgc
      541 tgacggttat tgttgggtga tggtgtcagg gcaccaaaaa aaaccgccga acatgtcggc
      601 ggctttctga cttacaaact gatagcttac atcagcggca ttgctaaccg cacaatgcta
      661 atcagcggtt gtggccatac acccagcacc agtaccaaca gtgcagagat cagcaccacg
      721 ataccgcctg cgctgtactg ccagtttgac ggtgcatcgc gacccggttg ttccggggcg
      781 tgcaggtaca ggctcaccgc cacgcgcagg tagtagtaaa ggccgattgc cgaaccgaca
      841 accacggcgc ccaccagcca ccacaagtgt gcctggacac cgaccgccag cacgtagaac
      901 ttaccaataa agcccagcgt catcggaata ccggccagag acagcatcat caccgtcatc
      961 accgccgcga ggatcgggcg atgccagaac agaccgcggt aagagaacag ggaatcagca
     1021 tccgggccac ggtacgggct ggacatcagg ctgaccacgc cgaacgcgcc gaggctgctg
     1081 aacagataac cggccaggta aactcctacc gcttccatcg acatctcgcc cgtttgcagc
     1141 gcaatcagcg ccaccagcag atagccgagg tgagagatag atgagtaacc gagcagacgt
     1201 ttgatattgg tctggctcag cgccatcagg ttaccgaaga tgatagaggc aaaggcgata
     1261 atcgccagca ctacccgaat cgcttcgctg tcacccaccg gtgcgtagag gaacagacgc
     1321 atcaccacac cgaagatagc gattttgctc gccgtcgcca ggaaagtgga aaccggcgca
     1381 ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg gcaccagaga gagtttgaag
     1441 ccgaggccaa caatcatcag tccgaaacct gccagcaaca gcggctcgtt gagcataccg
     1501 tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc cagactgtgc atacaccagc
     1561 gccataccaa acagcaggaa agaagacgct gcggcagaaa ggatggtgta tttgatactg
     1621 gcttccagcg aacgtttctg gcggaaagcg taaccgacca ggccaaacag cggcaaagag
     1681 atcagttcaa taccgaggaa caaagacgcc agatggttgg cattcgccag caggatcccg
     1741 cccagcgcgg caattaacac caacaggtag aactcatcct tgttgtcgtt atagccttca
     1801 agccacgggt aggcgaaagt acaggtggcg aggctcgcca acaataccag cccggtgtaa
     1861 agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc cgcctggccg
     1921 acaaaccaga gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt agcgttgagg
     1981 aaatgattgc gtcgccacgc aatggagagc atcacaacca ccaccgtcaa gccgacgatc
     2041 agcaacggta gcagtgcgat caggttttgt ggagttattg tcatggcgat ttacggcctt
     2101 gtagtagtaa cggaattaac aaaccactgc tggatattgc caatcgcgga gtgcgaggta
     2161 tccagaatcg gctgcggata gaagcccagc agtaccagca gcaccaccag caacaggatc
     2221 ataaacagct cacgcagcga catccctggc agttcctggc tggcaatctg gcttttcgct
     2281 ttaccgaagt aagcgcgatg caacatcgcc agcgaataaa cagatgcaaa gaccagccca
     2341 aaggtagaga taacggtaat caccgggaca acctggaagc tgccgaacag aatcataaat
     2401 tcgccgacga agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac
     2461 agtgctggca gccatttcat cttgctccac agaccgccca tcatgcgcat gtcgcgggta
     2521 tggatacgtt cataaagctg accacaaaga ataaacagac ccgccgccga caagccgtgc
     2581 gcaatcatct ggattaccgc gccctggtag gccaactggc tgccggtgta gatagcaatc
     2641 agcacgaagc ccatgtggga aaccgaggtg taggcgatca gacgtttgat atcggtctgg
     2701 gcgaaagcca tccacgcacc gtagaagatg ccgataaccc ccagccacat agcgattggc
     2761 gcaaactctg ccgatgcgtt cgggaacagc ggcagggaga aacgcagcaa accgtaagcg
     2821 gcagttttca gcaagatccc cgcgaggtca acggaaccgg cggtcggagc ctgagagtgc
     2881 gcatccggta gccagccatg cagcggaacc accggcattt tgactgcgaa ggcgatgaag
     2941 aagcccagca ttaacaggta ttccacacca ctggacattg gcgtattcag cagctcttca
     3001 tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa aaaccagcgc caggatggca
     3061 atcaacatca ccaggccact cgcctgggtg taaatgaaga acttggttgc cgccgtgata
     3121 cgcgttttac cgtcagaggc tttatgcccc cacagtgcga tcaggaagta catcggcacc
     3181 agcatcattt cccagaagaa gaagaacagg aacatgtcga tggcaaggaa cacgccgata
     3241 acgccgccca ggatccacat caggttgagg tggaagaagc cctgatattt ttcgatctct
     3301 ttccacgaac atagtaccgc cagcacaccg agcagaccgg tcagcacgac catcagcagc
     3361 gacagcccgt caatggcgag atgaatagag ataccaaaac gcgggatcca cggcatgtcg
     3421 aattcagact gccactgcgg aattccggcg gattgcgtca gtgaataacc gccctgcaac
     3481 cacagttgca gcgacagcgc cagcgtcaat cccatggtga tcagcgcgat ccagcgtggc
     3541 accttgacgc caaagcgttc ggtctgccag cacaggaagc cgccaataaa gggaattaat
     3601 attagccagg gtagtaacat ggcgatcttt attccttgta aaagtcccgt caggaccgga
     3661 ttttcaacga attctcacga cagaaaattc tcttcggact gggggtagtg tcggatgcac
     3721 cgcgaaggcg gcttatccga cctacgttct tatatgccca ggggcaatcc cacaatcctt
     3781 aactcaacgc agtaccatca acagtgccag caccacgacc gcaccgatgc tcatggatgc
     3841 cacataccag cgcagatagc cgttctcact taacagcaga cctttacctg caaagcggga
     3901 aaggacagcc gggatgttca tcattgagtt cagcggatcg cgtttcagca accaggcaat
     3961 acccaggaac ggcttgacga acactttgtc atacagccag tcaaatcccc aggcgttgta
     4021 ccaccaggtg cccagcagac ggcccggcgc actgttggcg atggaggtca ccagagtacg
     4081 tttacccagc cacagccagg ctgccagcag aatgccgacc accgcgacca cgccagaggt
     4141 aatttccagg gtcaacatgc tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg
     4201 cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg atcagcagca caatcagcgg
     4261 caggctgtga gttacccctt tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac
     4321 gatgaaaatc atacggaagg tgtagagcga ggtcataaac gcaccgacca gacctgccac
     4381 catcagattg atatgaccat tcgccatcgc acccgcgagg atctcatcct tactgaagaa
     4441 gcccgcagtg accagcggta gtgccgacag tgctgcgccg cccaccagga agcagagata
     4501 aaccagcgga atagatttac gcagaccgcc catcttgaag atgttctgtt cgtgatggca
     4561 ggccagaatg acggaaccgg atgccaggaa cagcagcgct ttaaagaacg cgtgagtcat
     4621 caagtggaaa atcgccgcat cccatgcctg cacgccaagc gcgaggaaca tgtagccaat
     4681 ctggctcatg gtagagtaag cgagaacacg tttgatgtcg gtctgtacca gcgcggcaaa
     4741 accggccagc agcagcgtaa ccgccccgac aatacccacc agatgcagaa cttccggcgt
     4801 catcaggaac aggccgtggg tacgggcgat caggtagaca cccgcggtta ccatcgttgc
     4861 ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc gcgtcagcaa gccatgtctg
     4921 caacggcaac tgcgcagatt taccgaccgc accgcccagc agcatcagcg tcgcccacat
     4981 cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt tccaccattt cgcggaagtt
     5041 cagggtgccc agttcgttgt aaagaatgaa cagtgcgaaa gcgaggaaaa cgtcacccac
     5101 acgggtcacg acgaacgctt tcattgccgc tgcgccattc ttcggatcgg tgtaatagaa
     5161 accgatcagc agataggagc acaggcccac gccttcccag ccgaggtaca tcagcagcag
     5221 gttatcggcg agaaccagaa ctaccatgct ggcgatgaac aggttggtgt aggcgaagaa
     5281 gcgagagtag ccctcttcac cgcgcatgta ccaggaggcg tacatgtgaa taaggaaacc
     5341 cacaccagtg accaccgaga gcatggtcag cgacaggccg tccagcacca ggttaaaacc
     5401 gatgttaaag tcgcctaccg acatccacgt ccacagcggc tggctgtatg actgctcgcc
     5461 gttagcgaag aaatcaacgc cgataaaggc ggttaccagc gccgccaagc ccacagagcc
     5521 aacgccgacg atcgccgaaa cgttttcaga ccagcgccca cgggagaacg ccagcaggac
     5581 gaagccaatc aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg catctcactt
     5641 actgaatcga tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg
     5701 atactcgctt ctgccgccgc gaggctgatg gcgagaatgt acatcacctg accgtcggtc
     5761 tgaccccagt agcttccggc gaccacaaag gccagcgcgg aggcgttaat catgatttcc
     5821 agaccaatca gcataaacag cagattgcga cggataacca gaccggttaa gccaagaacg
     5881 aataagattg ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt
     5941 ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc acccgcacgc tcttcacgac
     6001 cgacgtggaa ggccacaacc agacccgcga gcagcagcat agaagccagt tccaccgcca
     6061 gtacgtaagg cccgaacagc gtaataccca ctgctttagc actgattggc gtaccgtcga
     6121 taccctgatc attaacacca aggatggcgt aaacaatcac caccagcatg atggccgaca
     6181 aaattgccgg accaatccac acctgcggtt tcagccactg gcgttcctgt tcgatttctg
     6241 aaccgcccag gttgagcatc atcaccacga acacgaacag caccataatg gcacccgcgt
     6301 agacgataat ttccagcgca ccggcgaagt aagcgcccag tgagaagaac accccggaaa
     6361 tcgccagcag cgaaataatc aggtacagca gtgcgtgtac cggattggta tgggtgatca
     6421 ctcgcaaggt cgcaagtatg gctatcaggc cacagatata aaaagcgaac tccattgccc
     6481 ctctccttac ggtaacaggc tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc
     6541 cttatctttg ccgtcgattg ccatacctgc catccggtag aagttatatt ccgggtattt
     6601 gcccggaccg gagatcagca gatcctcttt ttcgtaaacc agatcctggc gcttgtattc
     6661 ccccatttcg aaatccgggg ttaactgaat cgccgtggtc ggacaggctt cttcgcacag
     6721 accacagaaa atgcagcgtg agaagttgat gcggaaaaat tccgggtacc agcgaccgtc
     6781 tttggtttct gctttttgca gcgagataca gccgaccggg caggctaccg cgcagaggtt
     6841 acaggctacg caacgctctt cgccgtccgg gtcgcgggtc agaacgatac gaccacgata
     6901 acggggcggc agatagaccg gctcttccgg gtacattcgc gtttcgcgtt tggcgaacgc
     6961 gtgcaggccg atcatccaga tactacgaac ctgggtgccg aaacctacta acaattcttt
     7021 taaggtcatg gtcttattgc cccttattgc gcctgccaga gaatgacagc cgccgttacc
     7081 agcaagttga tcagcgtcag cggcaggcag attttccagc cgaaggacat tacctggtca
     7141 taacgcggac gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt
     7201 ttcagcgcga accagatgaa tggcggtaac aacgggcctt gccagccacc gaagaacagc
     7261 gtcaccatca atgcagagat ggtcacaatc ccgatgtact cacccacgaa gaacagaccg
     7321 aacttcatac cggaatattc aatgtggtaa ccatccgcca gttcctgctc ggcttccggc
     7381 tggtcaaacg ggtgacggtg acataccgcc acgcccgcga tggcaaaggt aataaaacca
     7441 aagaattgcg ggataacgtt ccacacatgc gcctggctgt tgacgatgtc ggtcatgttg
     7501 aatgaaccgg cctgcgccac cacgcccatc agggaaagcc cgaggaacac ttcgtagctc
     7561 agggtctgcg cagaagcacg catcgcaccc agcaacgagt atttgttgtt acttgaccag
     7621 cccgcaaaca gcaccgcgta aaccgccaga cctgccatca tcaggaagaa caaaatcccg
     7681 atgttcaggt cggcaaccac ccaacccgga ctgactggca caatcgcaaa ggccagcagc
     7741 agcgaggtaa aggcaatcat cggtgccagg gtaaagatga cgcgatccga gaatttcggg
     7801 atccagtctt ctttaaagaa cattttgatc atgtccgcaa ccagctggag cgaaccgccc
     7861 cagccaacac ggttaggtcc gtaacggttc tggaacagac ccagcaggcg acgttcgcca
     7921 aagctcatga atgccccgca ggtgacaacc accagcagga tcaccaccgc tttgaggatg
     7981 gtcagcagga tctcaatcag ttccggtgat atccaactca ttgttgtgcc tccttgagat
     8041 cctcaagatg cgcgccagcc agcaccggag caataccgga catacccatc ggcaagccca
     8101 cctgccctgc cgtcagccct tcagcgattt caaccggcag cgtgaccgtg ttgccatcgt
     8161 aactaaagga gacgtgtgta cctgcgttca cacctaactt cgcggcatcc gctgggttga
     8221 gtttgatgta cggctgcggc atacggctct ggaagaccgg agcacgctgt gacaattcat
     8281 cgctgccaaa caggtggtaa tacggcgcga tacgccattt cccgtcctgc ggctggaagc
     8341 gtgccggtac gctggtgaag taatccagac cattttcgct ggtttcaaac agacgcacgc
     8401 ccggatcgcc aaagcgcagt ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg
     8461 gggagttcca gcccggcgcc caggcaaacg gcacttgcga acggtgcgcg gtcggctggt
     8521 tgttaccttc catcgagaag gtgaacatgg tgtcaatatc ctgcggctga cgcggctcat
     8581 gaacgctgat attggcgcgc atggcggtac gaccgctgta acggtgcggt tcacgggcca
     8641 gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc atctttgata cctgccagtt
     8701 ccgggatttt cgccacaaca gcgtcaatca catggtcgag ctgcgtccag tccacttcac
     8761 ggctcagcag ggtgctgtgc agcgagtgta accagcgcca gctttccagc atgacagttt
     8821 tgctgtcgta ataagcagga tcgtaaacct ggaagaaacg ttgggcgcgg ccttcgttgt
     8881 tgatcaccgt accgtcgctt tcagcaaagc tggcagcaga aagtaccaga tgggcgtttt
     8941 ccataatcgc tgtgcgttga tgatcaacca ccatcaccag cggcgcttta gccagcgcag
     9001 cattcacgcg ggtagcggaa gcgtgacgat gcagatcgtt ttccagcacc accaccgcgt
     9061 cggcgcgtcc ggtttccagt tcggtcaacg cttcttcaag cgaaccgcca cccataatgc
     9121 ccagccccat gctgttgacg gaacgggcaa tcatggtgat accaacgtca gcgccgcgac
     9181 ctttcagggc tttcgcgacg ttagccgcag cctgaatcac ctctatgcta ccggcgttcg
     9241 tcccggagat aatcaacggt ttcttcgcac ctgccagtgc ctgcacgatg acgtcgattt
     9301 tgctttgcag ctcaggttcg ataccgtcaa ccgctggcgc agagttatcc agcgcatggg
     9361 cgatggcaaa acctaaacgc gcctgatctt caaccggtgc gcggtaagtc cacgccgcga
     9421 tatcatccag acgggtgtca tcaacgttgg taacaaacag cggatgcttc gcacgttgac
     9481 cgatgttgag gattgccgca atctgccagt cagccacttt ctgtgctgcc gccatttcgc
     9541 gcgctttacc tttcacagcc tgacgcactg ccagcgcgac gcgcgcgccg gtctgggtaa
     9601 cgtcttcgcc cagcaccagt accgcatcgt aagattcgat ttcgcgcaga gccggagtat
     9661 aaatgccgcc ttcacgcagc actttcagcg ccagttgcag acgttcctgc tcaccgtgag
     9721 cgataccggt gtagaagttt tcttcgccca ccagttcacg cagcgcaaag ttgctttcca
     9781 cgctggcacg cggagaacca ataccgatca ctttcttcga ctgacgcaga atatctgccg
     9841 cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc atcgccacga cgctgtactg
     9901 gttgacgcgg acgatctttc aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga
     9961 ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat acgacgcagt tcgccgtaac
    10021 gttcaccggg gctgatgtta cagccgatgg aacattgctg gcagatgctc ggcgcaaact
    10081 gcatatccca tttacggttg taacgctcgg agtgcgtttt gtcggtaaat acgccggtcg
    10141 ggcaaatttc gaccaggtta ccggaaaatt cgctttccag cgtgccgtct tccgggcgac
    10201 cgaagtagac gttgtcgtgc gcaccgtaaa cgcccagatc cgtaccgtca gcgtaatctt
    10261 tgtagtaacg cacgcagcgg tagcaggcga tgcagcggtt catttcgtga gagatgaatg
    10321 gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc
    10381 cggtcatcac ggtcatatcc tgaagatggc agttaccgcc ttcttcacat accggacagt
    10441 cgtgcgggtg gttggtcatt aaccactcga ccacactttc acggaactgt ttcgcttctt
    10501 cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt catacaggac atcaccaggc
    10561 gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc acactggcgg caagcaccga
    10621 cgcttcccag cgccggatgc cagcaaaagt aaggaatatc aaggcccaga gacagacaag
    10681 cttccagcag gttgtccgct ccgttgacct cgtattcttt gccgtctaca tgaattgtag
    10741 ccattagcat gcttccagtt ttctcagtca gagactgagc gttaatcgaa attcggttac
    10801 cagcgctctt tcagcaggtt cggctgaatc ccattaatca aatgggtatt gctgaacggc
    10861 tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga tagcgctctg taacggctcc
    10921 actgcaccag gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg acacagttgc
    10981 tcaagtgttt cgatatcgcc cggctgacct tcaccacgct ccagcgcacg cagaattttc
    11041 acgctccacg gcagaccgtc gcggcacggc gtacaccagc cgcaggactc acgggcgaaa
    11101 aactcttcca ggttacgcac cagcgacacc atgttgatct catggtcaac cgccatcgcc
    11161 agcgccgtac ccagacggct tcccgcttta ccgatacttt cgaattccat cggcaggtca
    11221 aggtgcgctt cggtcaggaa gtcggtgccc gcgccgcctg gctgccaggc tttgaatttc
    11281 agaccgtcac gcataccacc ggcgtaatct tcgaggatct cgcgcgcggt agtaccaaac
    11341 ggcagttccc acaggcccgg atttttcacc cgaccggaga agcccatcag cttggtgcca
    11401 gcatctttac ttttcgagat gttctgatac cactccacgc cgttagcgag gatcgccgga
    11461 acgttacaca gggtttcgac gttgttgaca caggtcggtt taccccatgc gccggaggtc
    11521 gccgggaatg gaggcttcga gcgtgggtta gcacgacgtc cttccaggga gttgattaac
    11581 gctgtttctt cgccgcagat gtagcgccct gccccggtat ggacgaacag ttcgaaatcg
    11641 aaacctgttc ccataatgtt tttgccaagc aagcccgctt cggtggcttc ggcaatggca
    11701 cggcgcagat tcactgccgc ttcgatatat tcgccacgca ggaagatgta gccacggtaa
    11761 gctttcagcg caaacgcgga gataagcata ccttccacca gcaggtgcgg cagttgctcc
    11821 atcagcaggc ggtctttata ggtgcccggc tccatttcat cagcgttaca cagcaggtaa
    11881 cggatgttca tggattcgtc tttcggcatc aggctccatt tcaggccagt cgagaagccc
    11941 gcgccgccac gccctttcag accagagtct tttacctgat taacgatttc gtccggagac
    12001 agcccggtca gcgccttacg cgcgccttcg taaccgtttt tgctgcggta ttcgtccagc
    12061 cacaccggct gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg
    12121 ataatgtttt tcatttatac cgctccagca gttcagggat cgcttccggg gtcagatgcg
    12181 cgtgagtatc ctcatcgatc atcatgtttg gccctttatc acagttcccc aggcagcaag
    12241 ttggcagcag cgtaaagcga ccgtcaaatg tcgtttgccc tggtttgatg tttagctttt
    12301 tctcgagcgc cgcctgaata ccctgataac cgttgatatg acagaccacg ctgtcgcaat
    12361 aacggatcac atggcggcca accggctggc ggaagatctg gctgtagaac gttgccacgc
    12421 cttcgacgtc gctcgccgga atacccagca catcggcgat cgcgtggatc gcgccatccg
    12481 gcacccagcc acgctgcttc tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt
    12541 cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc agctcaaaag
    12601 cctcggtttg tggttgttga ttctcgtgca taattagcgg tccacatctg acataacaaa
    12661 atcgatactg cccagataaa caatcaggtc agacaccagg ctgccgcgga tcgccgccgg
    12721 aatttgctgc aaatgcgcat aactcggcgt gcggatacga gtgcgataac tcatggtgct
    12781 accgtcgctg gtcaggtagt aactgttgat ccccttggtc gcctcaatca tctggaaaga
    12841 ttcattggca ggcatcaccg gcccccacga cacttgcagg aagtgggtga tcagggtttc
    12901 gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc agcgggtgat ccgctttgaa
    12961 cgggccttcc ggcatgttgt tgaggcactg ctcaagaatg cgcagactct ggcgcagctc
    13021 ttccactttc agcattacgc gggtgtagca gtcagaaaca ccaccaccta ccgggatttc
    13081 aaagtcgaag ttttcatagc cagaataagg acgcgcttta cgcacgtcga agtcgatccc
    13141 ggtagcacgc aggcccgcgc cagtggtgcc ccactccagc gcctctttcg cgccataggc
    13201 ggcaacgccc tgagaacgac ctttcagaat ggtgttttgc agcgccgctt tctcgtaaga
    13261 cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc aggcgatccc agccgcgcgg
    13321 caggtcgtgc gctacgccgc caatacggaa ccacgccggg tgcatacgga aacccgtgat
    13381 cgcttccacc agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca cgggcgtcat
    13441 tgcgccgacg tcctgaataa aggtcgagat gtacagcagg tggctgttga tacggaacag
    13501 ttcggagagc ataacgcgaa tgacgttaac gcgatccggc acggtgatcc cggccagttt
    13561 ctctaccgcc agcacgtaag gcatttcgtt aacgcagccg ccgaggtatt caatacggtc
    13621 ggtatacgga atgtagctgt gccaggactg gcgctcgccc attttctccg caccacggtg
    13681 gtggtagccg atgtctggta cgcagtcgac aatctcttcg ccgtcgagtt gtaaaacgat
    13741 acggaaagcc ccgtgcgccg acgggtggtt cggaccgagg ttgaggaaca tgaagtcctc
    13801 gttttcggtg ccgcgcttca tcccccactc ttccggtttg aaggtcaggg cttccatctc
    13861 cagatcctgt ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc gcgccgggta
    13921 atctttacgc agcgggtgac ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg
    13981 gtgaccgtcg aaagtaatgc caaacagatc ccaggtttca cgctcatacc agttagcgtt
    14041 cgggaacagt ttggtgaagg tcggtacgtg caggtcgttt tctgccagcg ccaccttcag
    14101 catgatgtcg cggttacgat cgatagaaat cagatggtag aaaacggaaa aatccgcggc
    14161 aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat
    14221 gacgtaaggt ttcggcagtt tctttaagaa atcgccaact tccagtaatt gttcacgctt
    14281 gatccacaca acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa
    14341 acggttgcgc agttcgccaa tcaccggatc atcaagatga tcgcgggtct gccaggcggg
    14401 ttcttgcgcg gttaagtcgg tcatattgtt caccattgca aatggtccgt ggtgactgtc
    14461 ggcgcagtaa gcttcgcgaa atagatttag gaataagcga agaaaaatcc ctttgccgac
    14521 aggcgcaaat taaatctcgt caggtgtacg caggttggtt acggcaatgc gttcaccgcg
    14581 cttgcgttcg cgctctgatt gcatattggc gcgataaacg ccctgatcgc caaccaccca
    14641 ggagagcgga cgacgttctt tgccgataga ttcctgcaac agcatcagtg cctgcatgta
    14701 cgcttcagga cgcggcgggc agcccgggat atacacatca accgggatga atttatcgac
    14761 gccctgcaca acggaataaa tatcgtacat accaccagag ttggcacagg cacccattga
    14821 gataacccat tttggttcca gcatctggtc atacagacgc tgaataaccg gtgccatttt
    14881 ggtaaagcag gttcctgcaa ccaccatcag gtcagcctga cgcggcgaag cacgcaatac
    14941 ttctgcgcca aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca tctcaacgta
    15001 acagcaggaa agaccgaagt tatacggcca aattgagttt ttacgacccc agttaaccat
    15061 gtcattgagc ttgcccataa acacgttttt gttaacttct tgctccagag ggtcggttac
    15121 gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg ttgggatcta tgcgggtgag
    15181 cgtataatcc atcttaatgc ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc
    15241 cgggttcata cgctcgcggc gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac
    15301 cagataaacc agacctgcca gtaacacaaa aataaaaatt gcagcttcca caaagcctac
    15361 ccagccgctt tcgcggatag aggttgacca tgcgaacaga tacagcgctt caacgtcgaa
    15421 gataacgaag aacatggcca ccagataaaa cttggcggac aggcgtaagc gggcggagcc
    15481 gaccgagtcg ataccggatt caaacggcac gtttttcgac ctcgcgcgtg cgcgaccgcc
    15541 taaaaaccaa ccgcctacca gcatcaggca acacaggcca atggcaacga taagaaagat
    15601 agcgaatgcc cagtgatgag cgatgacttc agtggatgtt gacatactca ttgcttactc
    15661 atcaaaagta gcgccagatt cactgctctt cacggcagat ggacgccaca tcgattcatg
    15721 gggaggaata aaaaaaacct tacaatcact gtagaaattc ttttatacag ctaattgatg
    15781 tggtctttta ctcctttcta taaccttttg tcaactttaa caaaagtttc ttcacattag
    15841 tttacataat atcaacacca ttagcattta atgccctttc accccagatc cttgacgact
    15901 ccaggataat tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt
    15961 ctattttgac aggaatttgt gtcgattcct ccccccaaaa gagagtattt tcttgatctg
    16021 tgacacgctt ttgtcattcc ataacaaaaa cgcagcaaca aatttacgta ttttttaaca
    16081 tcattgtagc aggtgatttt tttcaggcga ttatttgtgc gttcgggacg tgaatctctg
    16141 gtgattgaaa aatgaacagt tttgtacgtt ctgcactatg cgaggaagga tttttactaa
    16201 aaaaaagccg ctggggttta aaacaccccc agcggctcgt tttttacact attgtctcag
    16261 gaattatcta tcgtccgtcg attactcgat atccctttca atcaacaatg aatcatcccc
    16321 ttccggagca gacattgggc tgtattgcca cggattatgg tagctctcca tcgcctgata
    16381 gatcacctgg gccagctcat tattactgga cgggtcatag cacagcaaat attcggtatc
    16441 aggtagcggc ggtaagccat ctactccact cagcacacgc aggtccgggc tcatcatctc
    16501 aaccggcctt gccgtcacgc caagaccggc tttcactgcc gcacgaacgg ccggaagcgt
    16561 cgaggcgaca taagccagtc gccatggaat atctgcttta ttaagcgtcg ccagcaccat
    16621 atcgcgaaac ggacttggat catccagcaa tacaagaggg atcggctcgc ctttttgcaa
    16681 tacgtattcc gctgcgcagt accagtgtgt tggcgaggtt cgcaggttca atgccttaaa
    16741 cgctgaagga cgatgggtgg ttaccatcaa atccacttcc tgggattcca gcatttctgc
    16801 catataggca ttacgtttaa cgcggacatc cagcgctaat ttcggataaa ccgaactcac
    16861 gcgatttaac aggaaaggta agatcgtatc ggcagattca tctgaagcac cgatagttaa
    16921 cacgccctga agattactga acattaatga tgagcaggcc tcatcattaa aacgcaggat
    16981 tttcctggcg taaccaagaa gttgaatgcc atgttcagtt aacagtttgt tgcgaccgtg
    17041 acgagcgaac agttctttcc caacgagttg ttccagacgc tgcatttgct gacttacggc
    17101 ggactgagta cgacacacag cggcagctgc ggcagcaaaa gtgttcagat cggcaacagc
    17161 aacaaatgtt ctcagcagat cgaggtcgag gttaattatc ggacgatttg cacttatcat
    17221 atattatcac ttactggcgg ctcatactga gctggttaat gctgtgcaca cacaaacaag
    17281 caattccatt tgtaatgtgc ctccctggca gtttcatccg gaacccggac gaaagtaaaa
    17341 atgcatatga gttgcactaa aaaagcgact cacattgttc cgttataatg cctgaagtag
    17401 atcacagaat atatcttcag ggatcgcata tctattaagt tactcactct tttctattta
    17461 tgacatgcgc gtgtttgtat aaatgtaaat gtgagtcctt gttccactct cgtgcagcat
    17521 cgctggtcat acgcgaacac gtacaaacag caatggtgag gcggcatcaa gagcagggat
    17581 ccgtcattta tgcgagcatt ttaccccaaa aaccttttat ttataagggt cattgcgaat
    17641 tatctgatgc aaagttatgt tatgttaggc aaagtaatct tctattatta ataagcacat
    17701 caaaaccttt ttgaatatta aataataatt aattagcatc atcctcattc attaattccg
    17761 cttaacaata gtttcacacc ttgcccccat tacgacacct cggaaatcat cacgctgtta
    17821 aactctccgt aatgcggaaa tatcatcatc gcgtttcatt agtgaattct taaatgaggc
    17881 attttcacac aattatctta cagataaaaa aaccagactt ac
//
LOCUS       DAZFRN010000097        17804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFRN010000097 DAZFRN010000000
VERSION     DAZFRN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.097"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(17..1360)
                     /gene="gdhA"
                     /locus_tag="ACHKUB_003197"
     CDS             complement(17..1360)
                     /gene="gdhA"
                     /locus_tag="ACHKUB_003197"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO6656176.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRLMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGS
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1596..1868
                     /locus_tag="ACHKUB_003198"
     CDS             1596..1868
                     /locus_tag="ACHKUB_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO6656177.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(1834..2241)
                     /gene="nudG"
                     /locus_tag="ACHKUB_003199"
     CDS             complement(1834..2241)
                     /gene="nudG"
                     /locus_tag="ACHKUB_003199"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO6656178.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            2328..2948
                     /locus_tag="ACHKUB_003200"
     CDS             2328..2948
                     /locus_tag="ACHKUB_003200"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6656179.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            complement(2957..4264)
                     /gene="ynjE"
                     /locus_tag="ACHKUB_003201"
     CDS             complement(2957..4264)
                     /gene="ynjE"
                     /locus_tag="ACHKUB_003201"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO6656180.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4331..4984)
                     /locus_tag="ACHKUB_003202"
     CDS             complement(4331..4984)
                     /locus_tag="ACHKUB_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6656181.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(4984..6519)
                     /locus_tag="ACHKUB_003203"
     CDS             complement(4984..6519)
                     /locus_tag="ACHKUB_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO6656182.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(6492..7658)
                     /locus_tag="ACHKUB_003204"
     CDS             complement(6492..7658)
                     /locus_tag="ACHKUB_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6656183.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(7668..8216)
                     /locus_tag="ACHKUB_003205"
     CDS             complement(7668..8216)
                     /locus_tag="ACHKUB_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO6656184.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8216..8923)
                     /locus_tag="ACHKUB_003206"
     CDS             complement(8216..8923)
                     /locus_tag="ACHKUB_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6656185.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(8938..9615)
                     /gene="ydjY"
                     /locus_tag="ACHKUB_003207"
     CDS             complement(8938..9615)
                     /gene="ydjY"
                     /locus_tag="ACHKUB_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO6656186.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(9620..10330)
                     /locus_tag="ACHKUB_003208"
     CDS             complement(9620..10330)
                     /locus_tag="ACHKUB_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6656187.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(10497..11303)
                     /gene="xthA"
                     /locus_tag="ACHKUB_003209"
     CDS             complement(10497..11303)
                     /gene="xthA"
                     /locus_tag="ACHKUB_003209"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO6656188.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            11749..12969
                     /gene="astC"
                     /locus_tag="ACHKUB_003210"
     CDS             11749..12969
                     /gene="astC"
                     /locus_tag="ACHKUB_003210"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO6656189.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            12966..14000
                     /gene="astA"
                     /locus_tag="ACHKUB_003211"
     CDS             12966..14000
                     /gene="astA"
                     /locus_tag="ACHKUB_003211"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO6656190.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            13997..15475
                     /gene="astD"
                     /locus_tag="ACHKUB_003212"
     CDS             13997..15475
                     /gene="astD"
                     /locus_tag="ACHKUB_003212"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO6656191.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQVLSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            15472..16815
                     /gene="astB"
                     /locus_tag="ACHKUB_003213"
     CDS             15472..16815
                     /gene="astB"
                     /locus_tag="ACHKUB_003213"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO6656192.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LCARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDILSQLLNLGSVYPFQREGGGNG"
     gene            16808..17776
                     /gene="astE"
                     /locus_tag="ACHKUB_003214"
     CDS             16808..17776
                     /gene="astE"
                     /locus_tag="ACHKUB_003214"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO6656193.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         4145 a         4656 c         4858 g         4145 t
ORIGIN      
        1 atcaggcatt tacaacttaa atcacaccct gcgccagcat cgcatcggca accttcacaa
       61 aaccggcaat gttcgcgccc tgcacgtagt tggtttgctc accttcacca ccatgctcaa
      121 cacaggcatg gtggatatcc agcatgatgt gatgcaaacg tgcgtcaact ttctcggctt
      181 tccagcccag gcgcgcagcg ttttgcgcca tttcaaggcc tgacgttgct acaccgccag
      241 cattagccgc tttacccggt gcaaacagta cgcctgcctg ctggaacagt tcagtcgctt
      301 cgatggtggt cggcatattt gccccttcag cgacggcttt aacgccattg gcgataagct
      361 gatgcgcggc gtcaacatcc agttcgttct gggtggcgca aggcagggcg atatcaaccg
      421 gtacagacca cggctgttgg ccttcgagat agaccagacc aaattctttg gcgtaatctg
      481 ccactcgacc atcgcggctg gctttgattt cgataagacg tgccagtttc tctttcgtga
      541 atccgctttc atcaactaca gtgccgctgg agtctgacgc agtgatcaca cgagcaccaa
      601 attccatcgc tttttcgata gcgtactggg cgacgttgcc ggagccagaa acggaaacgc
      661 gcatcccttc aaaacccata ccgtggcgtt ttaacattgc ttctgtgaaa taaaccagac
      721 cgtagccggt agcttccggg cgaataagac taccgccaaa tgaaaggccc ttaccggtga
      781 agacgcaggc ggtattgttg gagagctttt tcatcatccc cgccataaag ccgacttcac
      841 gaccaccaac gccgatatca cctgccggaa cgtcggtatc cgagcccaga tgacgataaa
      901 gctcggtcat cagcgcctgg cagaaacgca tcacttcgcc ttcgctttta cctttcgggt
      961 caaaatcgct accgccttta ccaccgccca tcggcagggt agtcagggca tttttgaagg
     1021 tttgttcgaa gccgaggaat ttgagaatgg agaggttaac tgacggatgg aagcgcatac
     1081 cgcctttgta cgggccgatg gcagagctga actgcacacg ccatgcacgg ttgacctgta
     1141 cctgattgcg atcatcaacc cataccacgc gaaactggat cacgcgctcc ggttcaacca
     1201 gacgctccag taatgacatc aggcgatatt ttggattttg ttcaagaaaa ggccagagtg
     1261 tggtcattac ttcacgaacg gcttgcgcga actcggtttg attcgggtcg cgcttttgga
     1321 catggttgag gaatgactcc agagaatatg tctgatccat agatataaaa cccttatata
     1381 ttaatacgat tgtgcttatg ttgtttatgt gatgtgcttt tgcagttttc gactatacca
     1441 tcaagaatgt gacggtaagc aagaaaaaaa tgacccagga aagcaaatta ataacgagag
     1501 taatctcata accaaatgcg ataactcaga cgctaaagtt ttgccacccg gctaccgtta
     1561 tcagtagcag agacaataac gttaaggagt tccctgtgag tcgagcattg ttcgccgtag
     1621 tattagcatt tccattaatt gcactggcta acccgcatta tcgtccggac gtggaggtaa
     1681 acgtaccgcc agaagtgttc agttcaggcg ggcagtcagc gcagccctgt actcagtgct
     1741 gcgtttatca ggataaaaat tattctgaag gcgcggtaat taaagccgag ggaatactgc
     1801 tgcaatgcca gcgggatgac aaaaccctta gcactaatcc gctggtctgg cggcgcgtaa
     1861 agccataaac gcctctaata atggaatgtc agcaggggcc agcggatatt gcagcgcctc
     1921 ttcaggtgag caccagacca gcgcctgatg ttcatgtgcc tgtaacgtcc cgtggaagtc
     1981 gggtacgtgc caggcatgaa gatggataat ccggcccgaa acttctcgct gatggctggt
     2041 aacatactca cccacagttg cttcgatgcc cagttcttcg cgtaactcac gcaccagcgc
     2101 ctgccgctgg ctttcatccg gctcgacttt accaccggca aactcccata atcccgcctg
     2161 atcgctctgg gcggggcgtt gcgcgagtaa aattttgcca tcacgttcaa tgatggcggc
     2221 aacaacttca atcattttca taaattacac acgggcagat aagcgaaaaa tgccataata
     2281 tccgcactgt tgctcaatca gaaaggcttt atatttaagg agtgcctgtg ctagaccgcc
     2341 atcttcatcc ccggattaaa ccgttgttgc atcagtgcgt gcgggttctt gataaaccgg
     2401 gcattacgcc agatggttta acattagtgg gatttgccat cggcgtgctg gcactgcctt
     2461 ttctggcgct gggctggtat ctggcggcgc ttgtcgtcat tttgttgaac aggctgcttg
     2521 atggtctgga cggtgcgctg gcgcggcgca gagggcttac cgacgcgggc ggctttcttg
     2581 atatttctct cgattttctt ttttacgcgc tggtaccttt tgggtttatc ctcgccgcac
     2641 cgcagcaaaa cgccctggca ggcggctggc tgctgtttgc gtttatcggc acaggcagca
     2701 gttttctcgc ttttgctgcg ctggcggcga aacatcagat tgataatccc ggttatgcgc
     2761 ataaatcgtt ttattatctg ggcgggttaa ccgaaggcac agaaacgatc ttactgtttg
     2821 tgctgggatg tttattccct gcatggtttg cgtggtttgc gtggatcttc ggtgcgttgt
     2881 gctggatgac gacctttacg cgggtgtgga gtgggtatct gacgctgaag tcgcaccagc
     2941 gtcagtaatt gcaatgttat ttgctactgt ccgggccgcg ttcaccggtt gctaccggat
     3001 tttttggatc gctgctccat tcgtaccagc cgccgtcata cacggaaaca ttcttccaac
     3061 ccatggcgcg tgcgtacata aaggtttcgg acgcgcgcca gccggtgccg cagtagaatg
     3121 aaacttgctg ctctggtttg atattccacg ctttccacat agcggtaata tcatcggcgc
     3181 tgcgcatggt gccatccggg ttatgaaaat cttccatatg cgtcgagtcg ctaccagcgt
     3241 gtccccaacg tgctccggct atttcacctt ttggtttaat atagctgtaa ccgctggtcg
     3301 taccgataaa ttctggccac gaacgaatgc tcaccagcga tgcatcctgg cgatgcagca
     3361 gtccacgggc ctgttccata tcgagcatca actgcggttg tgccgggatc ttcacgccga
     3421 aatccggttc cgctttcact ttcggtggcg ttcctcgctc aacaggcagt cccgcgtcgg
     3481 accaggtttg ccagccgcca tccagcagac gcacatcttt cacgcctgcg tagagcataa
     3541 tttgcgccac acgcgctcca gcgtatacgt cacgcccgta cagaatgacc gtggtgtcat
     3601 ggcgaatgcc gtgttttgcc agcatcgctt ttagttgttc atcagaaact ttgttccaca
     3661 gcggttcgct ttccacttcg ttggtatcga tgtagtcagc gccaggaata tggctgataa
     3721 ggtaaagctt aggagcgccc caggccgctt caatgacttt ccagtcaccg gcaggtttcg
     3781 ccgtaacctc tttaccttgt tgcaggtcgt gcagccattg cggataaacc agctgctcaa
     3841 agtgcggcag tttttgtaga cgggaaggtt cgcttagcgc gtcactcaga atggagatat
     3901 gcgtaaaacc tgctttttgc agtcgcgttt tgacggcgtc gacatctttg tcattaccgt
     3961 acagcgccac cggggcatcg gctttcaggt tatgttgctt gatccacgcg ttgagctgtt
     4021 cggtgctcat tttgtcgagc cagctggcag agaggtttaa ggcggcaggt tcatgaccag
     4081 aagggccatt taaggtttgt ggccagccgt tataaaacgc gctggggcga gtatctatcg
     4141 ctttgccatt ttgttgttga agctggtcaa gtgtaagagg cttcgccagt tcagcggccc
     4201 acgaagaagc gcaagccagc cctaaagcca ttgccagcgc ggtcatttga gaaacacgtt
     4261 tcatcgaaaa tcccgacatt gtagttttga gtgcgccatt ctgaactgac gcattgagaa
     4321 aaactttgcg ttatcgcagt ttgttgtaat tttctgacca ctgcgccata tccagaacag
     4381 aactatcagc aggaacatcc tggagatcgt gcgttacctg aacgacgggg atcgccagtt
     4441 cgcgaacttc gctgaacacc cactggcgaa aattatcgcg cagagccaca tcaagacggc
     4501 tgaatggctc atccaggagt aacgcttttg gctgagcgag aagggcgcgc agcagggcca
     4561 cccgcgctcg ctgaccgcct gataatgtcg cagggtcctg gtgataagtt tcagccagtc
     4621 ccgctcgatc aagcgcgtct ttcacggcat tgcgcctggc agtccccttg agcgtcgacg
     4681 gtagcgccag cagtaaattt tgcccgacgc tgaactggtc aaataacagt gcatcctgga
     4741 aaagaatgcc aatctgacgc tgtgcggtgg gtagcatgtc aatccgttgc tcattgagcc
     4801 atagctcacc tgtacaagaa aactgtccag ccagtgcacc aatcatccat gaaaacagag
     4861 tggattttcc acagccagac ggccccatta acgtgacaat gtcaccttta tccaccgtaa
     4921 agttaacgtt tgtcagcaag cggctttctg gtaaacgtag cgaaacattt ttcacgcaga
     4981 gcattagcgg agtccttgtc tgacataacc tacccatttt gcgactaacg cggtcagggc
     5041 aaaaataata agcggtaata gcagttgcca taaagcctgg gcggcgaaaa taccattgct
     5101 gccgccgctg cttaatgcca ccgcctcagt ggtgagcgtc ggaaaacgcc ccgcgcccag
     5161 ccacagcgtc ggcatatact gcgcaatact gactgaaaat cccaccgcga aggcaatcag
     5221 cgtagggcgt agcataagcg gacatttcac gtaaaagaag attttggccc gcgaccagct
     5281 cagtgtttgc gcaatcaaaa ttaaccgtga atcaatgcgc tgccaggcgg gttgcaggat
     5341 aaacagcatc cacggcatta cccacagcag atgcccccag accaccgccg tccagcttcc
     5401 atccaggttc agccatagcg ccagcgtgta ctggcccgcc accagtggca gagcgggcaa
     5461 tagaatgggc aaccagagcc acaactggcg acgctgtggc ccccattcca gccacagcag
     5521 taacaggagc aaagcgatga atgtcgccac cagccccatt gtcaggctgt tgatgagtgc
     5581 ttcactattg atcgtcgact gatccgcgag gatcgccagc agaaccacac acagcacacc
     5641 ggttaagggt aaaaaactcg ccagcgtagt gcctggtaat aaaggtgtgg caggcttgcg
     5701 aatgccatct acgcggggaa tagtgcgccg ccagctgcgc catagcagat agctcagcaa
     5761 aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt tcgtttgttg
     5821 atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg gtggattacc
     5881 tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa tcgccaacat
     5941 cgccattgcc agtgcaggtg cgacggaggg caagagcagc cagttcaggc attgccagcg
     6001 gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt cgctcaatac
     6061 tgccgctaag atccacagca gaaatgcgct ttctttcact gcaagggtga ggcccagccc
     6121 aatacccaat ttgtccatta gcggagtgaa atacgggaaa tagtcataaa gcagccctcc
     6181 gtcagcaaag agcagaaggg cgctggtggc aaaagccaca tggggaatgg cgagcagcca
     6241 cggcagacgg gcacacatac gctgccattt cggccaaggc cacagcgcca caatcaccag
     6301 cagggcaatc aacaacgccc cgaccgccgc gatggttgtc gacaccagcg ttgccagtaa
     6361 tgcctgcggt aactgtggat cggcaaataa cgcctgccag tgtgtcaacg acaaagcagg
     6421 cgatatcagc gtgagagctg ccgggatcag tggtgcataa atcaccgcca ccatcgccca
     6481 cagcaaaaag attaatgcgt accgtaacgg tgtagccatt cttgttccag cgcatttacc
     6541 caacctgcgt gcggttcagc cagtaccggc ggcagatctt gcggcattct tgattgcaat
     6601 gtttcgcgct gcccatcagg cagtttttgc ggatcgagaa cagaaggatc gccccagaca
     6661 gcgggatccg ctttacgcag ttgcgcattg ggtgagagca ggaagttggc aaccaccttc
     6721 gccgcagcac tggcatttgc gttagcagga atggtgacga aatgcacgtt gccaatcatc
     6781 ccctggttaa aaccaaaact gtaactgctt gcaggcaaat cgccgctggc gattttttgc
     6841 tgggcatgag cagggttaaa ggtcagcgac aggcgcaatg tgccggcttt cagcagagca
     6901 tccatccgcg cgggcgaagg cgggaaatcc tttcctttgc gccataaata gggatgtagc
     6961 gcgtcaagat attgccacaa gggcgcagtg acgcgggcga aagtggcttt atccggggca
     7021 acttttaacg cggcgggatc gggcgtcagc ataatcagca actgttcaag aaacgccgtg
     7081 ccggtaaagt ccggtgggcg cggataggta accgtgccgg gattggcttt agcaaactcc
     7141 agtaaggctt gcggcgtttg tggtggctgt ggcgtaacat cgcggcgggc gataaacgtc
     7201 agttgtgcgc cgccccaggg cgattcagcc ccttctgtag gcactgaaaa atcttcccgc
     7261 accggcagct gtgtgtcgac atagcgccag ttgggcagag tctccgccca gcccgtttgt
     7321 aataaattgg cctcttttaa ggtgcggaag ttttcgccgt tcacccagag cagatccacc
     7381 gagccgcccg ttttacgtcc ggcagcagct tcggtctgaa tgcgcttcac cgcgtctgcg
     7441 gcatccgcca gacgaacaat cttcaggttt atagcgtaat gggttttcat ctcgccgcta
     7501 acccagtcga gatagcggtt aattgcggta tcgccgcccc aggcgttaaa ccagacggtt
     7561 tgccctttgg cctcattttt aatttcctgc cagtcactgg catgtgttgc cagagaaaac
     7621 agcgataaca atcccagcaa ccacccacaa tggcgcatct tctctcctta agaacgtttt
     7681 tccggaatac ggcacagccc ctgagcggga atgtccatgg cagaattaaa acgagccgac
     7741 aggttttgca ggccgatcaa tgccgtcagt tcggtgagcg cctgagcgtc aaaatgcgca
     7801 gccagtcggg tgcgcagggc atcatcgacc gttggcggcg ttacgcttgc ggcttcggcg
     7861 tactccagcg ccagccgttc ttcatcgctg aagagcgggc tttgccgcca gtcagccact
     7921 gccagcagtt tatcggtgct gccggtacgc tcggcgactt tcatactggt gatatccacg
     7981 caaaactcac acaggcacat ttgcgcaatg cgcgcgctga caagcgatcg cactaccgga
     8041 tcgagcggtg agcgtttgcg ctccagccac ccaacaaaca ttgacaccaa ataaaagata
     8101 aacgggatcc gcccccacca gcgaatcggg cttagcacct cgccgtaatg gcgacgatgc
     8161 agccacgcct gcggacgtaa aatgaaagga attttgctaa gcggcggtaa acccatcagg
     8221 cattcctctt ctggcgctca agccatattt tcttcgccat aaaaatcacc acggtcaggg
     8281 caaacagaat aaacagtccg gtgacaaacc agaaagtacc gcctgttaac atgctgcccg
     8341 cccaggaata aacaatagtc gccggtaatt gaccaagccc ggtggcgata aaaaacgagc
     8401 gaaaacgtat tgaagtcaga ccggcagcat agctgattgg atcgaaaggg acaaaaggca
     8461 ataaccgaca gaccaggatg gtgtgtttgc cgtagcgagt gaaaaagccg tccatactgt
     8521 caagcacggt tttgccggtt aatttttcca ccacttcgcg gcccatcact ctggcgataa
     8581 aaaagcacag cgccgcgccg gccatcgaac tggtccacga cagcaagccg ccccagaacg
     8641 cgccaaacag cgacgcattg gcaaaggtga tcaaaaacgc aggtagcggt gcagcaatgg
     8701 cctgtaaaat catcaacaag aacgagacaa ctgccgccag tgcgccgtaa gactgaataa
     8761 agcgttctat accctgttgg tccacggcgg caaacgccgc aacgctgcga ttgataaact
     8821 catggacgcc gggaagcagc gcccatgcga gcagcatagc gaacaacagg atgatgaggg
     8881 ttatacggta gtaccagatt ttacgcgatt gtatcttcat catcctttat cctggcttta
     8941 ttcggtgatt ttaaaggtaa ccgttgccag cgtgttatcc gccgggagaa ctgaggcatt
     9001 gcctttgaat ttcacttcgc cgcgtttttc caccgcacca taagtgtatg ttgcattgct
     9061 gacgatgccg accgggcagc tatccagaca caccaggcaa cccgttttct tctcttctgc
     9121 tgccgttaaa ttaccgccaa agcgcatgtc cagtttcttg ccattactgt caacaatcac
     9181 ttcatcgaag gaatacgctt ttgccgcccc ttgccagttg accgaaatat ccagtttgct
     9241 gcctgtgaca tgagtcgttt ctttattatc catcgtcatg ttttcgcccg gcgtaccacc
     9301 tgcctctttc agggcttcat aaaatgcttt cggggtcgca taagccataa acagcgattt
     9361 atgtccgttg gagccatctt taaacacaat accgtgacga gtgtcgtcgg tgaggaaacg
     9421 accattaatt tgcaccagca tggttaccgt atggttattg gcatcgacgc gcagcggttg
     9481 gctgttcgaa agtccgtcgt attccacttt tgccgccgca ttctcttttt gatcgcagcc
     9541 gctaagcccg gcaacagcca gtaagcagag tgcagccagt ccttttttcc atgacactga
     9601 ataatgttgc gacatcgttc tatccttcat tttttgggtg agtaagtggg ctgcggcgcg
     9661 aagcagacag atccacatgt ttgtgtcggg cgtagagttt tgcgagctgg acgagaataa
     9721 acagcgccag gcccgccaga cagagttgta aaataaagcg cagcgtaatg ccttcattgg
     9781 cgagatcgct tgccatcacg gtataaataa caataccggg cagggtcgtg agtgccgaaa
     9841 taagggtata aggccagaag gcgatggtgg ttaatccgta agcgtaattt tgaatattgt
     9901 aaggaaacaa cgggattaag cgggtcagaa taagaaaatc aataccgtta cgcgcaatgc
     9961 ctttttcaat ggcctggaag gtatggctat ggcccacgta tttcagcagt aaatcacgtc
    10021 ccagccagcg cgccagcagg aatgagcacg aggaggccag cgtggcggca attaatgaga
    10081 gtagtgtccc taagagcggg ccaaaaacta ttcctccagc gatcaccagt atgcttcctg
    10141 gtaacagaaa gagagtggca ataatgaata acagaatata gaggctataa ccgaacaatc
    10201 cgctctggcg gatgagtgtc tgtaagtggg gtaaatcggt gagcagatcg aataaaccga
    10261 aagtgtggat agcgtaaatg accagcgcaa aaataagaca ggccaaaaga aatttacgct
    10321 cagcgttcat cgtggagttt cctatatcaa acttgataat agagaagtgg tcagacaact
    10381 aaaaacgcag tgacggtata acaaaggacg gcaggcaaca aatcattact gacagtttgt
    10441 tgatttttct caaggttaat tctcctgacc cagtttgagc caggagagct gctaaattag
    10501 cggcggaagg tcgcccagac gggggcgtga tcggacggtt tttccatgct gcgaatttca
    10561 tagtcgatgc cggtttctac gcaacattct gccagcggtt ggctggcgag cagcaggtcg
    10621 atgcgcagac cacggttatc gtcaaaacct tttgagcggt aatcaaacca tgagaaacga
    10681 tctgctgttt gcggattagc atggcggaag gtatcgacca acccccagct catcagcctg
    10741 tccatccatt cgcgctcttc cggcaggaaa gagcatttac cggtacgcag ccagcgctta
    10801 cggttttctt cgccaatgcc gatatccaga tctgtagggc tgatattcat atcgcccata
    10861 atcagtaccg gattatcacg tttgagttcg gtttccaggt agttttgcag attctgataa
    10921 aactgcgctt ttgccgggaa ttttatcgga tggtcgcggc tttcaccctg cgggaagtaa
    10981 ccgttgatca cggtgacatt acccagcagt gaggggattt ccgccataat aatccgccgc
    11041 tgcgcctctt cgtcgtcacc gggaaagccg cgacgcacgg caatcggcgt ctctttggtc
    11101 agcagcgcca cgccataatg gcctttctgc ccgtgataaa acacgttgta gccgagcttc
    11161 gccacctctt cgagcggaaa catatcgtca tgaacttttg tctcctgcag gccaatcaca
    11221 tccggttggt gcttttcgac gatggcttca agctggtgag gtctggcgcg caggccgttg
    11281 atattaaaag agacaaattt catagtcgct gccatttatt cagataaaga gtgcgtggat
    11341 ggtagcagaa tttcgcgttg cttaccgcct gttgttaagt gatttgatcg atggttgtta
    11401 atgatgtcaa cgatggcgca aaaaatgccc gcttttggtg cgcgctgcgt cagaatggcg
    11461 cagtaatttc cagtaaattt cgtcaatgat cacaattcca gaattatatt ttacttctgc
    11521 ataacattgc gttttttatt ctttttattc acccacatcg caaacgtatt cactttatat
    11581 gcactttaaa tgcatatggt ttggttataa ctccttgatt tccagttagc ctccgccgtt
    11641 tatgcacttt tatcactggc tggcacgaac cctgcaatct acatttacag cgcaaacatt
    11701 acttattgtt aacatataaa taacgaatta tttactgtag aggtcgctat gtctcagcca
    11761 attacgcgtg aaaactttga tgaatggatg atacctgttt acgctccggc accctttata
    11821 ccggtacgtg gcgaaggttc gcgcttgtgg gatcagcagg ggaaagagta tatcgacttc
    11881 gcgggtggca ttgcggtgaa cgcgctgggc catgcgcatc cggaactgcg tgaagcgctg
    11941 aacgaacagg cgagtaagtt ctggcatacc ggcaacggtt acaccaacga gccggtactg
    12001 cgactggcga aaaaattgat cgacgccacg tttgccgatc gcgtcttctt ttgtaactcc
    12061 ggtgcggaag ccaacgaagc ggcgctaaaa ctggcgcgta aattcgctca cgaccgctac
    12121 ggcagccata agagcggcat cgtggcgttc aaaaatgcgt ttcatggtcg cacgctgttt
    12181 actgtcagtg cgggtgggca gccagcctat tcacaggatt ttgcgccact gccggcggat
    12241 attcgtcatg ctgcatataa cgatattaac tctgccagcg cgctgattga cgactctacc
    12301 tgtgcggtga ttgtcgaacc catccagggg gaaggcggtg tggtgccagc cagcaacgcg
    12361 tttttacaag gtctgcgtga attgtgtaac cgccacaatg cgctgttgat ttttgatgaa
    12421 gtacaaaccg gcgtcgggcg caccggggaa ctgtatgcct atatgcacta cggcgtgacg
    12481 cctgatctgt taactaccgc caaagcgctg ggcggcggtt tcccggtcgg tgcgttgttg
    12541 gcaaccgaag agtgcgcccg cgtgatgacc gttggcactc atggcaccac ctatggcggt
    12601 aatccgctgg cctcggcggt ggcaggcaaa gtgctggagc tcatcaacac accagagatg
    12661 cttaatggcg ttaaacagcg tcacgactgg tttgttgagc gtcttaatac tattaatcac
    12721 cgctatggtt tgttcagtga agttcgcggc ttaggtttgc tgattggctg tgtactgaat
    12781 gccgattacg ccgggcaagc gaaacagatc tctcaggaag cggcgaaagc aggcgtgatg
    12841 gtactgattg cgggtggcaa cgtggtgcgt tttgcgcctg cgctcaatgt cagcgaagaa
    12901 gaggtgacga ccggactgga tcgctttgca gctgcttgcg aacactttgt tagccgaggt
    12961 tcatcatgat ggtcatccgt cccgttgagc gatcagatgt ctcggcgctg atgcagcttg
    13021 ccagcaaaac gggcggcggc ctgacgtcgc ttcccgccaa tgaagccacg ctttcggcgc
    13081 gtatcgaaag ggcaatcaaa acctggcaag gcgaactgcc caaaagtgag cagggctatg
    13141 tgttcgtgct ggaagatagc gagacaggca ccgtggcggg gatttgtgcc attgaggtgg
    13201 cggttgggct gaacgatccc tggtacaact atcgcgtcgg cacgttggtt cacgcctcaa
    13261 aagagctgaa tgtctataac gcattgccga cgctgtttct cagtaacgat cacaccggca
    13321 gcagcgagct gtgcacgctg tttctcgacc cggactggcg caaagagggc aacggctatt
    13381 tgctgtcgaa atcgcgcttt atgtttatgg cggcttttcg cgacaagttt aatgacaaag
    13441 tggttgctga aatgcgcggg gtgattgacg aacacggcta ttcaccgttc tggcaaagcc
    13501 tcggtaaacg cttcttttcg atggatttta gccgcgccga ttttctctgc ggcaccgggc
    13561 aaaaggcatt tattgcagaa ctgatgccga aacatccgat ctatacccac tttttatccc
    13621 aggaagccca ggacgtcatc ggtcaggtac atccgcaaac cgcgcctgcc cgcgcggtgc
    13681 tggagaaaga aggttttcgt taccgcaact atatcgacat cttcgacggc gggccaacgc
    13741 ttgagtgtga catcgaccgc gtgcgcgcca ttcgtaaaag tcggctggtg gaagtggcag
    13801 aagggcagcc tgcgcagggc gattttccgg cctgcctagt cgccaatgaa aattatcacc
    13861 atttccgcgt ggtgctggcg cgtaccgatc cggcaaccga gcgtttgatt ttaaccgccg
    13921 cacaactgga tgccctcaaa tgccacgcag gggatcgcgt tcgtctggtg cgcctgtgcg
    13981 cagaggagaa aacagcatga ctttatggat taacggtgac tggataacgg gccagggcgc
    14041 atcgcgtgtg aagcgtaatc cggtatcggg cgaggtgtta tggcaaggca atgatgccga
    14101 tgccgctcag gtcgggcagg cttgtcgcgc agcccgtgcg gcgtttccgc gctgggcgcg
    14161 gctctcattg gctgaacgtc aggtcgttgt cgaacgcttt gccggattgc tggaaaggaa
    14221 taaaggcgaa ttaaccgcga ttattgccag agaaacgggt aagccgcgct gggaagcggc
    14281 aaccgaagtg acggcgatga tcaataaaat cgcgatatca attaaggcgt atcacgttcg
    14341 taccggcgag cagcgtagtg aaatgccgga cggtgcggcg agcctgcgac atcgcccgca
    14401 cggcgtgctg gcggtgtttg ggccgtataa tttccctggt catttgccga acggacatat
    14461 cgttccggca ttgctggcag gtaacaccat tatctttaaa cccagcgaac tgacaccgtg
    14521 gagtggcgaa gcggtaatgc gtttatggca gcaggctggc ttgccgccgg gcgtactgaa
    14581 cctggtgcag ggcgggcgtg aaacgggtca ggtgctgagt gcgctggagg atctcgacgg
    14641 tttgctgttt accggtagcg ccaataccgg ctaccagctg catcgccagc tctccggtca
    14701 gccggagaaa attctcgccc ttgagatggg cggtaataat ccgctaatta tcgatgaggt
    14761 ggcggatatc gacgcggctg tccatctgac cattcagtcg gcgtttgtca cagccgggca
    14821 acgctgcacc tgcgcccgcc gtttattgct caaaagcgga gcgcagggcg atgcgtttct
    14881 tgctcgtctg gttgccgtca gccagcgatt aacgccgggc aactgggatg acgaaccgca
    14941 gccgtttatt ggcgggctga tttctgaaca ggccgcacag caggtggtta ctgcctggca
    15001 gcaactggaa gcgatgggcg gacgaaccct gcttgcgccg cgcttattac aatcagagac
    15061 atcgttgctg acgccgggga tcattgaaat gacaggcgtt gctggcgtac cagatgaaga
    15121 ggtgttcgga ccgttattgc gcgtctggcg ttatgattct ttcgaggaag cgattctaat
    15181 ggcgaataac actcgcttcg gactctcttg cggtctggtt tcccccgagc gggaaaaatt
    15241 cgatcaactg ttgctggagg cgcgggcggg gattgttaac tggaacaaac cgcttactgg
    15301 tgctgccagt accgcgccat tcggcggcat tggtgcatcc ggtaaccatc gccccagcgc
    15361 ctggtatgcc gcagattact gcgcatggcc tatggcgagc ctggagtcgg actcgttaac
    15421 tttgcccgca acgcttaacc ccgggctgga tttttccgat gaggtggtgc gatgaacgcc
    15481 tgggaagtta atttcgacgg gctggtaggg ctgacgcatc attacgctgg cctgtcgttt
    15541 ggtaatgaag cctctacccg tcaccgtttt caggtgtcta acccgcgaca ggcggcaaaa
    15601 cagggcttac tgaaaatgaa agcccttgcc gatgcgggat tcccccaggc cgtgatcccg
    15661 ccgcacgagc gtccgtttat tccggtgctg cgtcagttgg gattcagtgg tagcgatgag
    15721 caggtactgg aaaaagttgc acgccaggca ccgcactggc tttccagcgt cagttccgct
    15781 tcgccaatgt gggtagccaa tgcggcaacg atcgcgccat ctgccgatac gctggatggc
    15841 aaagtgcatc tcaccgttgc caacctgaac aataaatttc accgctcgct ggaagcgcac
    15901 gtcaccgaat cacttttaaa agcgattttt aacgacgaag agaaatttag cgttcattcg
    15961 gcgttgccgc aggtagcgtt gctcggtgat gagggggcgg caaaccacaa tcgtctcggc
    16021 ggtcattacg gtgagccggg tatgcaactt tttgtctacg ggcgagaaga aggcaatgat
    16081 acccggcctt cccgttatcc ggcgcgacag actcgcgaag ccagcgaggc ggtggcaagg
    16141 ctgaatcagg tgaatcccca acaggtgatt ttcgcccagc aaaacccgga cgttatcgac
    16201 cagggcgttt ttcataatga cgtgattgcc gtgagtaacc gccaggtgct gttttgccac
    16261 caacaggcgt tcgctcgcca gtcacagtta ctggcaaacc tgtgtgcgcg ggtgaacggt
    16321 tttatggcga tagaagttcc ggcaactcag gtttccgtgt cggatgcggt gtctacctat
    16381 ctgtttaaca gccaactgtt gagccgcgat gatggttcca tgatgttggt gctgcctcag
    16441 gagtgtcggg aacacgccgg agtatggggt tatctcaatg aactccttgc cgctgacaac
    16501 ccgattagcg aactaaaagt ctttgattta cgtgaaagca tggcgaatgg cggcggcccg
    16561 gcgtgcctgc ggttgcgggt ggtattgaca gaagaagaac gccgggcggt gaatccggcg
    16621 gtgatgatga acgatacgct gtttaatgcg ctcaatgact gggtggatcg ttactaccgc
    16681 gatcgcctta ctgctgccga tctggccgac ccgcaattgc tgcgcggagg gcgggaagca
    16741 ctggatatat tgagccaatt actgaatctc ggttcggttt atccgttcca gcgcgaggga
    16801 gggggcaatg gataattttc ttgctctgac cttaacgggt aaaaaaccgg ttatcaccga
    16861 gcgagaaatc aacggcgttc gctggcgctg gctgggcgat ggtgtgctgg aactgacgcc
    16921 attaacgcca ccgcaaggcg cactggtgat ttcagcggga atacacggta atgagacggc
    16981 acctgtggag atgctggacg cgttgcttgg cgcgatatct cacggcgaga tcccgttacg
    17041 ttggcggttg ctggtgatcc tcgggaatcc tcctgcgctg aagcaaggga aacgttattg
    17101 ccatagcgat atgaatcgaa tgtttggcgg tcgttggcag ctatttgctg aaagcggaga
    17161 aacctgtcgg gcgcgcgaac tggaacagtg cctggaagat ttttatgacc tgggcaaaga
    17221 atctgtgcgc tggcaccttg atctacatac cgcaattcgt ggctccttgc atccgcagtt
    17281 cggtgtatta ccgcaacgcg acattccctg ggacgagaaa tttctgacgt ggctgggtgc
    17341 ggcggggctg gaggcgctgg tgttccatca ggaacctggt ggtacgttta cccatttcag
    17401 cgccagacat tttggcgcgc tggcctgtac gctggaactt ggcaaagcgt tgccctttgg
    17461 gcaaaacgat cttcgccagt ttgcagtaac tgccagcgca attgctgcgc tgctatctgg
    17521 tgagagtgtc ggtatcgtga gaacaccgcc gctccgttat cgggtggttt cgcaaattac
    17581 tcgccactcg ccgtccttcg aaatgcatat ggcaagtgac acgctgaatt ttatgccgtt
    17641 tgagaaagga acattgctgg cgcaggacgg agaggaacgt tttaccgtaa cccatgatgt
    17701 agagtatgtg ttattcccta atccgttggt agcgttggga ttacgcgcgg gattaatgct
    17761 cgaaaaaata agctaatact tacccgcaga aatcattctg cggg
//
LOCUS       DAZFRN010000098        17575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFRN010000098 DAZFRN010000000
VERSION     DAZFRN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.098"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(1..1756)
                     /locus_tag="ACHKUB_003215"
                     /pseudo
     CDS             complement(1..1756)
                     /locus_tag="ACHKUB_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2280..4208
                     /gene="thrS"
                     /locus_tag="ACHKUB_003216"
     CDS             2280..4208
                     /gene="thrS"
                     /locus_tag="ACHKUB_003216"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO6656194.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4212..4754
                     /gene="infC"
                     /locus_tag="ACHKUB_003217"
     CDS             4212..4754
                     /gene="infC"
                     /locus_tag="ACHKUB_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO6656195.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4851..5048
                     /gene="rpmI"
                     /locus_tag="ACHKUB_003218"
     CDS             4851..5048
                     /gene="rpmI"
                     /locus_tag="ACHKUB_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO6656196.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5101..5457
                     /gene="rplT"
                     /locus_tag="ACHKUB_003219"
     CDS             5101..5457
                     /gene="rplT"
                     /locus_tag="ACHKUB_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO6656197.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5574..5698
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5580..5624
                     /gene="pheM"
                     /locus_tag="ACHKUB_003220"
     CDS             5580..5624
                     /gene="pheM"
                     /locus_tag="ACHKUB_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO6656198.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5908..6891
                     /gene="pheS"
                     /locus_tag="ACHKUB_003221"
     CDS             5908..6891
                     /gene="pheS"
                     /locus_tag="ACHKUB_003221"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO6656199.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6906..9293
                     /gene="pheT"
                     /locus_tag="ACHKUB_003222"
     CDS             6906..9293
                     /gene="pheT"
                     /locus_tag="ACHKUB_003222"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO6656200.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLAGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9298..9597
                     /gene="ihfA"
                     /locus_tag="ACHKUB_003223"
     CDS             9298..9597
                     /gene="ihfA"
                     /locus_tag="ACHKUB_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO6656201.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9698..10678
                     /gene="btuC"
                     /locus_tag="ACHKUB_003224"
     CDS             9698..10678
                     /gene="btuC"
                     /locus_tag="ACHKUB_003224"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO6656202.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10741..11292
                     /gene="btuE"
                     /locus_tag="ACHKUB_003225"
     CDS             10741..11292
                     /gene="btuE"
                     /locus_tag="ACHKUB_003225"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO6656203.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11292..12041
                     /gene="btuD"
                     /locus_tag="ACHKUB_003226"
     CDS             11292..12041
                     /gene="btuD"
                     /locus_tag="ACHKUB_003226"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO6656204.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12119..12583
                     /gene="nlpC"
                     /locus_tag="ACHKUB_003227"
     CDS             12119..12583
                     /gene="nlpC"
                     /locus_tag="ACHKUB_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6656205.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12830..13543
                     /gene="ydiV"
                     /locus_tag="ACHKUB_003228"
     CDS             12830..13543
                     /gene="ydiV"
                     /locus_tag="ACHKUB_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO6656206.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13606..15042
                     /gene="selO"
                     /locus_tag="ACHKUB_003229"
     CDS             13606..15042
                     /gene="selO"
                     /locus_tag="ACHKUB_003229"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO6656207.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLHRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(15046..15237)
                     /gene="hemP"
                     /locus_tag="ACHKUB_003230"
     CDS             complement(15046..15237)
                     /gene="hemP"
                     /locus_tag="ACHKUB_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO6656208.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15369..16415)
                     /gene="aroH"
                     /locus_tag="ACHKUB_003231"
     CDS             complement(15369..16415)
                     /gene="aroH"
                     /locus_tag="ACHKUB_003231"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO6656209.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16572..17405)
                     /gene="ppsR"
                     /locus_tag="ACHKUB_003232"
     CDS             complement(16572..17405)
                     /gene="ppsR"
                     /locus_tag="ACHKUB_003232"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO6656210.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         4471 a         4020 c         4586 g         4498 t
ORIGIN      
        1 gaacagctga catgttttca tgatttttag cggccaacag tgtgaataat tgacgttgac
       61 taaaagaata tttttttgca aaagtactca gtgttgatat ataagataac acgacgtctt
      121 tattaccctt gctcatggcg atgtataaag caggggttcc atgtgctagc tttgctcaac
      181 ttgtatttaa aggcgatgcc attaatttta gagaggaacc gtgtgacaca caaagggtga
      241 ttattttcca ttgcggcgta taatcctggc tcaccatcat gtattttcat ggctaaaata
      301 ctcatcgcca gttttttgtt taaaagctct tgtgctattg gagtggttaa tgcattcaga
      361 aagatatcga ttatttcatc gaatccatac aacatggaga tataaagtcc aggtgtttcg
      421 ttagcacttt tagtttgcag taatttaacc agattatctt catgaatgat gtgattatct
      481 accagtgact gaatttcttg cataaatatt ttcacgttgt cagtatgtct gttcatcata
      541 gccaaaaata aagcaggtgt accaacgcta ttctgcgcag ttattatttc aatgatttct
      601 ttttcaggga gtatgggcaa ctgtttgaat aatgtaataa gctgttgagt ctggcctgta
      661 gccattatta acaacattgc cgatggatgt ataggggcaa atggcattcg tgatgcaata
      721 cccgcgaaaa tagcccctgg aactcccaca ggtaataggg gcagaatagc aattgcacag
      781 ttgcgaacat acctatcagc ccaggtctga gcatcgggac ctgaactgat gaacttatca
      841 agcgaaagaa agccccgtgc tgtcctgtgg gtgtctctga cggcaacgtt ggtatcgttc
      901 gggtcgtaaa cggacacaac atagtttaat tgtccataca ctgactgctt tattcgtagc
      961 cttactgaca aagtatgatt gtctatatat aaaatcgcgg acatatgact gacattgttt
     1021 gccatcattc gacaaaagaa aatatctaaa gcgaagccaa aatccttact cgcgataacg
     1081 cgactccctg caggaggatg gcgaagtaat gctctgaact ccgcgtcccg gactaagggg
     1141 acatgctgct gtatggattc aacggaggag taatgtttaa tcaggttgac tttgcctttt
     1201 ccatttctca atgaatcaag ggcgtattgc aatgacagat ggcgacaaaa aatagcgtca
     1261 gaaggagatt gcaaaaaaca tgcactacca ttgagattta ctgttgggtg tatagttttt
     1321 tctctatgga tttttttaga cagtttcata caatggttac gatcttcagc agttaactgt
     1381 tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg cgtggtttcc
     1441 agtaataagg aaatgaaatc tttttatagt gtaatgtaat gagtatattt tctgcatgga
     1501 tgggtgaagt ggcaaagtta atggtaaaaa gggcagtgga ataacaattt tgtgatattt
     1561 ttcgacagtt tgcggtaaat tcctgaataa cacagtatga cagcatcgaa cttaagtcag
     1621 aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct gatgaaggtg
     1681 ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt ctgataatga
     1741 tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg aggatgatga
     1801 ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt
     1861 ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc
     1921 aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa
     1981 acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct ggtgtactca
     2041 ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct cccttttttt
     2101 agtgttaaca tcgctcaacc gggtggaagg tgagaggtta atgtctttca tttgtgtacc
     2161 tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac tgttaataaa
     2221 caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa ggatataaaa
     2281 tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct gtaagcccca
     2341 tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta
     2401 atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg tccatcatta
     2461 ccgccaaaga cgaagacggt ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc
     2521 acgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt attgacaacg
     2581 gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc gaagcactcg
     2641 agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag aaagtcagct
     2701 ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtc tccattcttg
     2761 acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa tatgtcgata
     2821 tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa
     2881 cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt atttacggta
     2941 cggcgtgggc agacaaaaaa gcgcttaacg cttacctgca gcgcctggaa gaagccgcga
     3001 aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc
     3061 cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg gaagtgtttg
     3121 ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc atgatggacc
     3181 gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg ttcaccacgt
     3241 cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac gtacaaattt
     3301 tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag tttggtagct
     3361 gccaccgtaa tgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc
     3421 aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt aacggatgta
     3481 tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc gtcaaactct
     3541 ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct gaggcggacc
     3601 tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt gaaggcgctt
     3661 tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca tggcagtgcg
     3721 gtacagtaca gctggacttc tctttgcctt ctcgtctgag cgcttcttat gtaggcgaag
     3781 acaatgaacg taaagtaccg gtaatgattc accgcgcaat tttggggtcg atggaacgtt
     3841 tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt gcgccagttc
     3901 aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa ttgacgcaaa
     3961 aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag attggcttta
     4021 aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt gataaagagg
     4081 tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga agcatggacg
     4141 taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt aaacaattgg
     4201 aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg
     4261 gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag cttggtattg
     4321 tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta gtcgagatca
     4381 gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc ctctatgaaa
     4441 agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta
     4501 aattccgtcc tggtacagat gaaggcgact atcaggtaaa actccgcagc ctgattcgct
     4561 ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc
     4621 accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag
     4681 tggtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg ctcgctccta
     4741 agaagaaaca gtaaggcctt caagtagcac tgtctgtgga gccttcgggc ttcacaggct
     4801 ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa atgccaaaaa
     4861 ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc
     4921 acaagcacgc taacctgcgt cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc
     4981 tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt
     5041 acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag gagagcacat
     5101 atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa
     5161 gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca ggctgttatc
     5221 aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg
     5281 tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag caaattcatc
     5341 aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga tatcgcagta
     5401 ttcgacaaag tagcgttcac cgctctggtt gaaaaagcga aagcagctct ggcataagcc
     5461 agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa cattgacttt
     5521 tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa
     5581 tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag gaggctagcg
     5641 cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct ttttttgtat
     5701 gcgcgttttg aaaaataaaa agcctgtctc atcaggtgta attgtcgcag ccagtctgga
     5761 cacggccagc gcggagaaac cggagcgtat ttaagtacgt gagaatttcg agcactgccc
     5821 gggaccaaaa tggcaagtaa aatagcctga tgggataggc tctaagtcca acgaaccagt
     5881 gtcaccactg acacaatgag gaaaaccatg tcacatctcg cagaactggt tgccagtgcg
     5941 aaggcggcca ttagccaggc gtcagatgtt gccgcgttag ataatgtgcg cgtcgaatat
     6001 ttgggtaaaa aagggcactt aacccttcag atgacgaccc tgcgtgagct gccgccagaa
     6061 gagcgtccgg cagctggtgc ggttatcaac gaagcgaaag agcaggttca gcaggcgctg
     6121 aatgcgcgta aagcggaact ggaaagcgca gcgctgaatg cgcgtctggc ggcggaaacg
     6181 attgatgtct ccctgccagg tcgtcgcatt gaaaacggcg gtctgcatcc ggttacccgt
     6241 accatcgacc gtatcgaaag tttcttcggt gagcttggct ttaccgtggc aaccgggccg
     6301 gaaatcgaag acgattatca taacttcgat gctctgaaca ttcctggtca ccacccggcg
     6361 cgcgctgacc acgacacttt ctggtttgac actacccgcc tgctgcgtac ccagacctct
     6421 ggcgtacaga tccgcaccat gaaagcccag cagccaccga ttcgtatcat cgcgcctggc
     6481 cgcgtttatc gtaacgacta cgaccagact cacacgccga tgttccatca gatggaaggt
     6541 ctgattgttg ataccaacat cagctttacc aacctgaaag gcactctgca cgatttcctg
     6601 cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc cttcctactt cccgttcacc
     6661 gagccttctg cagaagttga cgtcatgggt aaaaacggta aatggctgga agtgctgggt
     6721 tgcgggatgg tgcatccgaa cgtgctgcgt aacgttggca tcgacccgga agtttactct
     6781 ggtttcgcct tcgggatggg gatggagcgt ctgactatgt tgcgttacgg cgtcaccgac
     6841 ctgcgttcat tcttcgaaaa tgatctgcgt ttcctcaaac agtttaaata aggcaggaat
     6901 agattatgaa attcagtgaa ctgtggttac gcgaatgggt gaacccggcg attgatagcg
     6961 atgcgctggc gaatcaaatc actatggcgg gcctggaagt tgacggtgta gaaccggttg
     7021 ccggtagctt ccacggcgtg gtcgttggtg aagtggttga gtgtgcgcag catccgaacg
     7081 ctgacaaact gcgtgtgaca aaagtgaatg ttggcggcga tcgcctgctg gacatcgtct
     7141 gcggtgcgcc aaactgccgt cagggcctgc gtgtggcggt agctaccatt ggtgctgttc
     7201 tgccgggtga tttcaaaatt aaagcggcga aactgcgtgg tgaaccgtct gaagggatgc
     7261 tgtgctcctt ttctgagctg ggaatttctg acgatcatag cggcattatc gagctgcctg
     7321 cggatgcgcc gattggcacc gacatccgtg aatacctgaa acttgatgac aacaccatcg
     7381 aaatcagcgt gacgccaaac cgtgccgact gtttaggtat cattggtgtt gcgcgtgacg
     7441 ttgccgtgct gaatcagctg ccgctggttg aaccggaaat cgttccggtt ggtgcgacca
     7501 tcgacgacac gctgccgatt acagtcgaag cgccggaagc ctgcccgcgt tatcttggcc
     7561 gtgtggtaaa aggcattaac gttaaagcgc caactccgct gtggatgaaa gaaaaactgc
     7621 gtcgttgcgg gatccgttct atcgatgcag ttgttgacgt caccaactat gtactgctcg
     7681 aactgggcca gccgatgcac gctttcgata aagatcgcat tgaaggcggc attgtggtgc
     7741 ggatggcgaa agagggcgaa acgttggtac tgcttgatgg cactgaagcg aagctgaatg
     7801 ctgacactct ggtcatcgcc gaccacaaca aggcgctggc gatgggcggc atctttggtg
     7861 gcgaacactc tggcgtgaat gacgaaacgc aaaacgtctt gctggaatgc gctttcttca
     7921 gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct gcatactgat gcgtctcacc
     7981 gttatgagcg tggcgttgat ccggcactgc agcacaaagc gatggaacgt gcgacccgtc
     8041 tgctgatcga catctgcggt ggtgaggctg gtccggtaat tgatatcacc aacgaagcaa
     8101 cgctgccgaa gcgtgcaacc atcactttac gtcgtagcaa actggatcgc ctgatcggcc
     8161 atcatattgc ggatgagcag gtaactgaca ttctgcgtcg tctcggctgc gaagtgaccg
     8221 aaggcaaaga cgagtggcag gcagttgcgc cgagctggcg tttcgatatg gagattgaag
     8281 aagatctggt cgaagaagtc gcgcgtgttt acggctacaa caacatcccg gatgagccgg
     8341 tacaggcaag cctgattatg ggtactcacc gtgaagctga cctgtcgctc aagcgcgtga
     8401 aaacgctgct caacgacaaa ggctatcagg aagtgatcac ctacagcttc gttgatccga
     8461 aagtgcagca gatgatccat ccaggcgttg aagccttact gctgccaagc ccgatctctg
     8521 ttgaaatgtc agcaatgcgt ctttctctgt ggaccggcct gctggcaacc gtggtgtaca
     8581 accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag cggtctgcgt ttcgtaccag
     8641 atactcaggc accgttgggc attcgtcagg atctgatgtt agccggtgtg atttgcggta
     8701 accgttacga agagcactgg aacctggcaa aagagaccgt tgatttctat gatttgaaag
     8761 gcgatcttga atccgttctc gacctggccg gtaaactgaa tgaggttgag ttccgtgcag
     8821 aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat ttatctgaaa ggtgaacgta
     8881 ttggttttgt tggggttgtt catcctgaac tggaacgtaa actggatctt aacggtcgca
     8941 ctctggtgtt cgaactggag tggaacaagc tcgcagaccg cgtggtgcct caggcgcgcg
     9001 agatttctcg cttcccggcg aaccgtcgtg acatcgcggt ggtggtcgca gaaaacgttc
     9061 ccgcagcgga tattttatcc gaatgtaaga aagttggcgt aaatcaggta gttggcgtaa
     9121 acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg gtataagagc ctcgccataa
     9181 gcctgatcct gcaagatacc agccgtacac tcgaagaaga ggagattgcc gctaccgtcg
     9241 ccaaatgtgt agaggcatta aaagagcgat tccaggcatc attgagggat tgaacctatg
     9301 gcgcttacaa aagctgaaat gtcagaatat ctgtttgata agcttgggct tagcaagcgg
     9361 gatgccaaag aactggttga actgtttttc gaagagatcc gtcgcgctct ggaaaacggc
     9421 gaacaggtga aactctctgg ttttggtaac ttcgatctgc gtgataagaa tcaacgcccg
     9481 ggacgtaacc cgaaaacggg cgaggatatt cccattacag cacggcgcgt ggtgaccttc
     9541 agacccgggc agaagttaaa aagccgggtc gaaaacgctt cgcccaaaga cgagtaatct
     9601 gatctaacta aaaaggccgc tctgcggcct tttttctttt cactgtcgaa gagtcaccgt
     9661 aaaatcaacg ccatgacact tcagcagaac ggataccatg ctgacacttg cccgccaaca
     9721 acagcgacaa aatattcgct ggttattatg cctgtcagtt ttgatgctgc tggcgcttct
     9781 cttaagcctt tgcgccggtg aacaatggat ctcgccaggt gactggtttt ctcctcgtgg
     9841 cgaactgttc gtctggcaga ttcgcctgcc acgtacgctg gctgtattgc tggttggtgc
     9901 ggcgctggct atatccggcg cagtaatgca ggcgttgttt gaaaatcctc tggcagaacc
     9961 tggactactt ggcgtctcta acggcgcagg cgtagggctt atcgccgcgg tattgcttgg
    10021 gcaagggcaa ctcccaaact gggcactagg gctgtgtgcg attgctggcg cgcttatcat
    10081 cactttaata ctcttacgtt tcgcccgtcg tcatctttcg accagtcggt tattgctggc
    10141 tggcgttgca ttagggatta tctgtagcgc actaatgacg tgggctatct acttttccac
    10201 ctctgttgat ttacgtcagc tgatgtactg gatgatgggc ggttttggcg gcgtagactg
    10261 gcggcaaagc tggctgatgc tggcattgat ccccgtgttg ttgtggatct gttgtcagtc
    10321 caggccgatg aatatgttag cacttggcga gatctcggcg cggcaactgg gtttacccct
    10381 gtggttctgg cgcaatgtgc tggtggcagc gaccggctgg atggttggcg tcagtgtggc
    10441 gctggcgggt gctatcggct ttattggtct ggtgatccca catattcttc ggttgtgtgg
    10501 tttaaccgat catcgcgtat tacttcccgg ctgcgcgctg gcaggggcga gcgcattgct
    10561 gctggccgat gttgtagcgc gcttggcatt agctgccgca gagctgccta ttggcgtggt
    10621 caccgcaacg ttgggtgcgc cggtgtttat ctggttattg ttaaaagcag gacgttagcc
    10681 gcaaaaagac ggtctatgat taaaagctaa gatttttact gaccacaccc aggagaaacg
    10741 atgcaagatt ccattctgac gaccgtagtg aaagatatcg acggtgaagt gaccacgctg
    10801 gagaagtacg ccggtaatgt gctgttgatt gtcaatgtcg cctcaaagtg tggcttaacg
    10861 ccgcaatatg agcagttgga gaatattcag aaagcctggg ccgatcgagg ttttgtggtg
    10921 ctgggattcc cgtgcaacca gtttctggaa caagaaccgg gcagcgatga agagattaaa
    10981 acttactgta ccaccacatg gggggtgacg ttcccgatgt tcagtaagat tgaagttaat
    11041 ggcgaaggac gccatccgct gtatcaaaaa ttgattgccg cagcgccgac cgcagtcgcg
    11101 ccggaagaga gcggattcta tgcccgtatg gtcagcaaag gtcgtgcacc gctgtaccct
    11161 gatgatattt tatggaattt tgagaaattt ttagttggca gggacggatt ggtcatccag
    11221 cgtttttccc cggatatgac gccggaagac cccattgtga tggaaagcat taaactggcg
    11281 ttggcaaaat aatgtctatt gtgatgcagt tacaggatgt tgcggaatct acccgcctgg
    11341 ggccactttc tggcgaggtt cgggctgggg agatcctgca cctggtgggg ccgaatggcg
    11401 cgggtaagag taccttgctt gcacgaatgg ccggaatgac cagcggtaag ggaagcattc
    11461 agttcgcggg gcaacccctg gaagcatggt ccgcaacaaa actcgcgctg catcgcgcct
    11521 atctttcaca acagcagacg ccgccgtttg caatgccggt ctggcactac ctgacactgc
    11581 atcagcacga taaaacgcgt accgaactac tgaatgatgt cgcaggggcg ctggctcttg
    11641 atgacaaact cggacgtagc accaatcaac tttccggcgg tgaatggcaa cgcgtacgtc
    11701 ttgctgcggt ggtgctgcaa atcacaccac aagccaatcc cgcaggtcaa ttgctgcttc
    11761 ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag tgcgttagac aaaattctga
    11821 gcgctctgtg tcagcaagga ctggcgattg tgatgagcag tcacgatctc aaccacacat
    11881 tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa aatgctggcc agtggacgca
    11941 gagaagaggt gctcacgccg ccaaatctgg cgcaggccta tgggatgaat tttcgccgtc
    12001 tggatatcga aggtcacaga atgctgattt cgaccatctg atttcattag tatcttgaaa
    12061 atcgggtaat tacgacgcta aattaatgca agaataaaaa acagaggaac cgtacggaat
    12121 gcgtttctgc cttattttga tcacagcact gtttctggcc gggtgtagcc accataaagc
    12181 accgccgcca aatgccagac tttctgattc gattaccgtt attgccggtt tgaacgacca
    12241 gctacaaagc tggcatggca cgccgtatcg ttatggtggc atgacgcggc gcggtgtgga
    12301 ctgttcggga tttgtggttg taacgatgcg cgatcgtttc gatttgcagc tgccccgaga
    12361 aaccaaagaa caagcctcta tcggcacgca aattgataaa gacgagttgc tgcctggtga
    12421 cctggtcttt ttcaaaacgg gttccggaca aaatggtttg catgtaggta tttacgatac
    12481 caacaaccaa tttatccacg cctctaccag caagggagtg atgcgttcct cacttgataa
    12541 tgtctattgg cagaaaaatt tctggcaggc gagacgaatt tagtatgtcg aaaaatggac
    12601 ggcgatgata catcgccgtc catttactcc tgaatacagt agtattttgc agacaaaaat
    12661 tccgtaaatg gattattcgt ttataatcag gataatctaa aaaaaggacc cctgaaaaag
    12721 tggttaacat ggaacaaatg aaagttactc cttatgtaat tgttatgtct tatggcctca
    12781 tcccttttag ccggatactg aaaaacatcc ttcgagaggg acggttacca tgaagatttt
    12841 tttggagaat ctttatcatt ctgattgcta tttcctgccg ataagagata accagcaaga
    12901 ccttgtcggc gttgagttaa ttacccattt ctccagtgaa gatggtacgg ttcgtatccc
    12961 gacgagtcgg gttatagcgc aacttaccga ggagcaacat tggcaattat tttcagagca
    13021 gttggaatta ctaaaatcat gccaacattt ttttattcaa cataaactct ttgcctggct
    13081 taatttaacg ccacaagtgg ctacattatt attagagagg gataattacg caggcgaact
    13141 attaaaatat ccgtttattg aactgttgat taatgagaat tacccgcatc tcaatgaagg
    13201 aaaagataat cgcgatcttt tatccttatc gcaaatgtat ccccttgtgt taggcaattt
    13261 gggagcaggt aatagcacaa tgaaagcggt ttttgatggt cttttcaccc gcgtcatgct
    13321 ggataaaagt tttattcagc aacaaatcac gcatcgctcc ttcgaaccgt ttattcgtgc
    13381 gatccaggcg caaatttccc cttgttgcaa ttgcatcatc gcggggggca ttgatacgac
    13441 agaaatattg gcacagatta cccccttcga tttccatgct ctccaaggat gcctgtggcc
    13501 tgccgttcct ataaatcaga taacgacgtt ggttcagcga taaccctcct gtttgctggt
    13561 gtttaagacg agagtaaccg tctacactat taaacaggag gagctatgac cctgtctttt
    13621 attacccgct ggcgcgatga attgccagca acctatacag cactttcccc tacgccttta
    13681 aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag tattccatcg
    13741 tcgctgttta aaaatggcgc aggtgtctgg ggcggcgaaa ccctactgcc tggtatgtca
    13801 ccactggcgc aggtttacag tggtcatcag ttcggcgtct gggcgggcca actgggtgat
    13861 gggcgcggta ttttactcgg cgaacaactg cttgctgatg gcactacaat ggactggcat
    13921 ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc ggttttacgt
    13981 tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat tccgacgacc
    14041 cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt ggagccaggc
    14101 gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga acatttttac
    14161 tatcgccgcg agccggaaaa ggttcgtcag ttagctgact ttgccattcg tcattactgg
    14221 tcatatcttg aagatgatga ggacaaatac cgcctctggt ttagcgatgt tgtcgcacgt
    14281 accgcatcgt taattgccca atggcagacg gtcggctttg ctcatggggt gatgaatacc
    14341 gataacatgt cgctgctggg gctgacgctt gattacgggc cgtttggttt tctggatgat
    14401 tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag ctttgataat
    14461 caacctgctg tcgcgttgtg gaatttacat cgtctggcgc agacattgtc accatttgtt
    14521 gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt gacgcattat
    14581 ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga taacgcgcta
    14641 ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg cacattccgc
    14701 atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgatga gtttattgat
    14761 cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca agacgaggtt
    14821 agcgatagtg agcgtcagca gctgatgcaa agcgttaacc cagctctggt gttgcgcaac
    14881 tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgacgga attgcaccgc
    14941 ctgcatgagg cgttgcgaaa tcctttcagc gacagagctg atgactatgt cagtcgtcca
    15001 cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact tggtcaacag
    15061 cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat cgataatcag
    15121 tttgccatcc ggacctaaca gtgtctggct ggaaattcgc cgaataggct gcggggaagc
    15181 ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat aacgcataaa
    15241 aattaataat aattgatata acaatgattc ttattatcaa tattatggct gaccagagca
    15301 agcagtaatg tgctgagaag acaacaagtt gcgcagaaat gcccatccca tcaggaatgg
    15361 gcacgcattc agaagcgggt atctaccgca gaggcaagtt tttcgaccag gcgttcggta
    15421 tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg ctgaccgccg
    15481 acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc aatcgccgta
    15541 gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg acggtgctgc
    15601 ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc aaactcgtgc
    15661 agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt gccgccacgc
    15721 ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg accatttttg
    15781 tctggcgaga gaaacatatg gctggcgcgg gctgcgcgga tagcatccac ggcaatccgc
    15841 gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgccga agccatttca
    15901 cggtggatct gactttcggt agtacgtgcg ccaatcgcgc cccaactgat taaatcagca
    15961 ataaactgac cggtcaccat atcgaggaac tcagtcgcgg tagggacacc cagctcattt
    16021 acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata gctgccgttt
    16081 aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt ttcaaaatag
    16141 gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg cagacgggtg
    16201 gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat gaccaacagt
    16261 cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt gacatgggtg
    16321 gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac caggctctca
    16381 atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa tttgttgctt
    16441 ctacggcagg actaccggga agtgatgcgc atcacgataa accaatcgca gctgatttca
    16501 agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa ataagctaat
    16561 gcactagttc tctagtacat tcggcggcta agccccatga tatcgaggat cttggtggca
    16621 atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt acggtacaac
    16681 gcttctactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg gctgttctcc
    16741 cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc gaacaattta
    16801 tgctgaagcg gtttgagcga cgcgggtagc accagattgt ccatatcgtc ggcaataaag
    16861 gggtagtttg ccgcgcggat accaaactgc attgccagat acagactggt gggggtttta
    16921 ccacagcgag aaacaccgag caggatcacc tgagcctggt ccagattgcg caacgaaatg
    16981 ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata tttattgaga
    17041 ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag tttcatctct
    17101 tgttgtagcg gggcaaccag cgcctgaaca atatcctggc aaaagccttc actttgcaag
    17161 atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg cacacctgtc
    17221 tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc attttcgaca
    17281 aacggcagcg taatgctgct gatagtgacg ggaaattgtg acattactgc gtgtcctaat
    17341 acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc aacagcatta
    17401 tccattttgt tcttcccgtg atgcagacat aattctatga aagcataaat taaaaacgca
    17461 cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcaatttta ttttttatga
    17521 aaacagcatt tcatttttat ggtttcgttt ataccgatgg tttatgtgga aattg
//
LOCUS       DAZFRN010000099        17471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFRN010000099 DAZFRN010000000
VERSION     DAZFRN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.099"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            102..356
                     /gene="bssS"
                     /locus_tag="ACHKUB_003233"
     CDS             102..356
                     /gene="bssS"
                     /locus_tag="ACHKUB_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO6656211.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            471..1589
                     /gene="solA"
                     /locus_tag="ACHKUB_003234"
     CDS             471..1589
                     /gene="solA"
                     /locus_tag="ACHKUB_003234"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO6656212.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1637..1750
                     /locus_tag="ACHKUB_003235"
     CDS             1637..1750
                     /locus_tag="ACHKUB_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO6656213.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2011..2577
                     /gene="yceJ"
                     /locus_tag="ACHKUB_003236"
     CDS             2011..2577
                     /gene="yceJ"
                     /locus_tag="ACHKUB_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO6656214.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2581..3156
                     /gene="yceI"
                     /locus_tag="ACHKUB_003237"
     CDS             2581..3156
                     /gene="yceI"
                     /locus_tag="ACHKUB_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO6656215.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PLATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3198..4250)
                     /gene="trhO"
                     /locus_tag="ACHKUB_003238"
     CDS             complement(3198..4250)
                     /gene="trhO"
                     /locus_tag="ACHKUB_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO6656216.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGIRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4475..5395
                     /gene="lpxL"
                     /locus_tag="ACHKUB_003239"
     CDS             4475..5395
                     /gene="lpxL"
                     /locus_tag="ACHKUB_003239"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO6656217.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5567..6793
                     /gene="mdtG"
                     /locus_tag="ACHKUB_003240"
     CDS             5567..6793
                     /gene="mdtG"
                     /locus_tag="ACHKUB_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO6656218.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6876..7250
                     /gene="msyB"
                     /locus_tag="ACHKUB_003241"
     CDS             6876..7250
                     /gene="msyB"
                     /locus_tag="ACHKUB_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6656219.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7251..7478)
                     /gene="yceK"
                     /locus_tag="ACHKUB_003242"
     CDS             complement(7251..7478)
                     /gene="yceK"
                     /locus_tag="ACHKUB_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6656220.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7651..10194)
                     /gene="mdoH"
                     /locus_tag="ACHKUB_003243"
     CDS             complement(7651..10194)
                     /gene="mdoH"
                     /locus_tag="ACHKUB_003243"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO6656221.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10187..11722)
                     /gene="mdoG"
                     /locus_tag="ACHKUB_003244"
     CDS             complement(10187..11722)
                     /gene="mdoG"
                     /locus_tag="ACHKUB_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO6656222.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12116..13273
                     /gene="mdoC"
                     /locus_tag="ACHKUB_003245"
     CDS             12116..13273
                     /gene="mdoC"
                     /locus_tag="ACHKUB_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO6656223.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13281..14762)
                     /gene="clsC"
                     /locus_tag="ACHKUB_003246"
     CDS             complement(13281..14762)
                     /gene="clsC"
                     /locus_tag="ACHKUB_003246"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO6656224.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14704..15237)
                     /gene="ymdB"
                     /locus_tag="ACHKUB_003247"
     CDS             complement(14704..15237)
                     /gene="ymdB"
                     /locus_tag="ACHKUB_003247"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO6656225.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15332..15643)
                     /gene="ymdA"
                     /locus_tag="ACHKUB_003248"
     CDS             complement(15332..15643)
                     /gene="ymdA"
                     /locus_tag="ACHKUB_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO6656226.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15764..16096)
                     /gene="csgC"
                     /locus_tag="ACHKUB_003249"
     CDS             complement(15764..16096)
                     /gene="csgC"
                     /locus_tag="ACHKUB_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO6656227.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16155..16607)
                     /gene="csgA"
                     /locus_tag="ACHKUB_003250"
     CDS             complement(16155..16607)
                     /gene="csgA"
                     /locus_tag="ACHKUB_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO6656228.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16648..17103)
                     /gene="csgB"
                     /locus_tag="ACHKUB_003251"
     CDS             complement(16648..17103)
                     /gene="csgB"
                     /locus_tag="ACHKUB_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO6656229.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(17232..>17471)
                     /locus_tag="ACHKUB_003252"
     CDS             complement(17232..>17471)
                     /locus_tag="ACHKUB_003252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656230.1"
                     /translation="FSAPPWTIFYCKKTRFVTACAQDISQQSATGKKNDCLVIFDSGK
                     RRFKRKQNIFCVDNSVFTWVLILWYEQKKKNTTRG"
BASE COUNT         4379 a         4434 c         4136 g         4522 t
ORIGIN      
        1 tcggtgaata cccttactta ttggtatact gaagttgctt cagataaaaa cgcattgaac
       61 ctcgaataac gttgtctagt aacacgaatt agggggccat gatggaaaaa aataatgaag
      121 tcattcagac tcatccgctc gtagggtggg acatcagcac cgttgatagc tatgatgcgc
      181 tgatgttgcg tttgcactac cagaccccaa ataagtccga gcaggaaggg actgaagttg
      241 gtcagacgct ctggttaacc actgatgttg ccagacaatt tatttcgata ttagaagcag
      301 gaatcgccaa aattgaatcc ggtgatttcc cggtaaacga gtatcggcgt cattaacatg
      361 acatgaccat ccacccaaaa ggcacctcac cggtgccttt accatttctg ggttgtatat
      421 ctgcattcag ttaattactc tgcttaaacc atgagagcag agagaaaaaa atgaaatacg
      481 atctcatcat tattggcagc ggttccgtag gcgctgccgc cgggtattat gcaacccgcg
      541 ccggtttaaa cgtgctaatg accgacgccc atatgccacc gcatcaacac ggcagccacc
      601 acggcgatac gcgattaatt cgccatgctt atggtgaagg cgaaaagtat gtcccgttgg
      661 tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca caacgaagat gatcccattt
      721 ttgtacgctc tggtgtcatt aacctcggcc cggctgactc cgcatttctc gccaacgtcg
      781 cccacagcgc cgaacagtgg caactcaacg ttgaaaagct cgatgcgcaa gggattatgg
      841 cccgctggcc agaaatacgc gtcccggaca actacatcgg cttatttgag actgattccg
      901 gttttttgcg cagcgaactg gcgattaaaa cctggatcca actggcgaag gaagcgggct
      961 gtgcgcaact gttcaactgc ccggtcaccg caattcgtca tgacgatgat ggcgtaacta
     1021 ttgaaaccgt tgacggtgag tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg
     1081 taaaagacct gctcccggag ctgcctgtcc agcccgtacg caaagtattt gcctggtatc
     1141 aggccgatgg gcgctatagc gtgaagaata aattcccggc gtttaccggt gaacttccca
     1201 atggcgatca atattatggt tttccggcag aaaacgacgc gttgaagatt ggcaaacata
     1261 acggaggcca ggttatccat tcagcggatg aacgtgttcc gtttgcggaa gtggtcagcg
     1321 atggttcgga agccttcccg ttcttgcgca atgtattgcc gggtatcggt tgctgcctgt
     1381 acggcgctgc ctgcacctat gataattcgc ctgacgaaga ttttattatc gataccctac
     1441 ccggccacga taatacactg ctcattaccg gcctgagtgg gcacggtttt aaatttgcgt
     1501 cagttttagg ggaaatagct gccgattttg cgcaagacaa aaaaagcgat tttgatttga
     1561 cgccattcag gctttcccgc ttccaataat catcattacg gcctccagaa tgaggccgtt
     1621 tttacaggag tatttaatgc gtcggctgtt gcactatctc atcaataata ttcgcgagca
     1681 tctgatgctt tatctttttc tctggggatt gttggcaatt atggatctga tctatgtgtt
     1741 ttatttttag ttttccgcac tgacataaac tgacaaatat ttaatctccc tttcatttag
     1801 cgtgaaaaat tgaatgagat aattcctgct tctacagcac tcaccagaca tctctgatgc
     1861 tgaacccact aaacttatga agattctttc attattaatt caaaattatt gaaataaaaa
     1921 taaatatcat tcaatttgtt atttttgtgt tgcaattcat tctccgacac gccattttct
     1981 ttctctcgca aattcgtttt ggatgcactt atgtcattca caaatacccc tgaacgctat
     2041 ggtgttatat ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct
     2101 ttaggtctgt ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa
     2161 ctgcataaaa gcatcggtat tttgttgatg atggggctgg ttattcgcgt tctgtggcgc
     2221 gtcatatctc caccgccggg tccgctgcag agttattcgc ccatgactcg tcttggcgca
     2281 aaggctggac atctcgcatt gtacttattg ctgttcgcca tcggcatcag cggctatctg
     2341 atctcaactg ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc
     2401 ctcgccgacg ctggcgcaca ggctgacttt gccggtgccc tgcatttttg gctcgcgtgg
     2461 agcgtcgtag tactgtccgt tatgcacgga tttatggccc tgaagcatca tttcatcgat
     2521 aaagacgaca ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa
     2581 atgaaaaaaa gcctgcttgg tttaaccttc gcttccctga tgttctctgc cggttcagcg
     2641 gttgccgccg attacaaaat tgacaaagaa ggtcagcacg cctttgttaa cttccgcatc
     2701 cagcatcttg gctatagctg gttatatggc acctttaaag atttcgacgg tacttttacc
     2761 tttgatgaaa aaaatccggc tgccgataaa gtgaatgtga caattaacac caccagcgtc
     2821 gatactaatc acgccgaacg cgataaacat cttcgcagtg cagatttcct caatactgca
     2881 aaatatccac tggcaacatt cacctccacc agcgtgaaga aagacggtga cgaattggat
     2941 attaccggcg atctgacgct gaatggcgta accaaacccg tcacgctgga agcgaaatta
     3001 attggtcaag gcgacgaccc gtggggtggt aaacgtgcag gcttcgaggc cgaaggcaaa
     3061 attaagctca aggacttcaa tatcaaaaca gatttaggtc cagcttctca ggaagtggat
     3121 ctgattattt cagtggaagg cgtacagcag aagtaagcgt gaaatgttgc cggatggcac
     3181 gcatccggca atgatattta ttctgttgga tcaggaatgc ccagtgttgt attcagacgt
     3241 ccacgagact tattaaagat cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt
     3301 tcttcaggcg gtaacgcgct ttcttcgcag caaatctcac tacaacaacc tttgtatttt
     3361 tccgcacata ctggacactg aataaaaagc aggtggcagc catcattttt acagttggta
     3421 tggctgtcgc acggcgcacc gcactggtgg caatgcgcga taatctcatc agagatacgt
     3481 tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa aacgcaccgg caagccctgc
     3541 tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga tatgccagac tttgttgaat
     3601 ccgttatgtt tcatccaggc actggctttt tcacaacgaa tgccgccggt gcagtacatg
     3661 acgatttttt tatctttatg tgcctgcatc atctcgactg cttttggcaa ctgctcacgg
     3721 aaggtatctg ccggaatttc cagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg
     3781 ttacgcatgt cgataaacag tgcatcggga tcgtcaagca tggcgttcac ttccgccgct
     3841 tgcaaatact caccaacatt actggcatcg aagtgaggat catcaatacc gtcggcaaca
     3901 atgcgatcgc gtaccttcat gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg
     3961 atattcaggc gtatgccctc taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt
     4021 tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc cctcatgcgc cagatacact
     4081 cgcccaaaaa cattcagcgc ggtaaacagc tgatataaag cgtcacgggt cgccttagga
     4141 tcggcgatgt ggaaatactt ataaaacgaa atggtggttc gcggttcgct ctcagccaac
     4201 attttggctt ttaacgcatc gttggaaatg cggttgtgta acactggcat ggtgtacggt
     4261 tcctgcgaga tgggaaagta aaaatccgcg gcatgatata gcaattatcg ataattaaca
     4321 tccacacatt ttacgctaca tttgcgcatt aaaaattatt tgttatttac aagcgcggca
     4381 atttcgccca gtcttcagcc acaaattttg gttgcgggcg aaaaaatgcg acaatacata
     4441 caattgcccg aataggttga aaaacaggat tgatatgacg aatctaccca agttctccac
     4501 cgcactgctt catccgcgtt attggttaac ctggttgggt attggcgtac tttggttagt
     4561 cgtgcaattg ccctacccgg ttatctaccg cctcggttgt ggattaggaa aactggcgtt
     4621 acgttttatg aaacgacgcg caaaaattgt gcatcgcaac ctggaactgt gcttcccgga
     4681 aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg
     4741 cctgatggaa accggcatgg cgtggttctg gccggaccgc cgaatcgccc gctggacgga
     4801 agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt
     4861 tggcatccat tttctgacac tggaactggg cgcgcggcag tttggtatgc aggaaccggg
     4921 gattggcgtt taccgcccga acgataatcc actgattgac tggctacaaa cctggggccg
     4981 tttgcgctca aataaatcga tgctcgaccg caaagattta aaaggcatga ttaaagccct
     5041 gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt
     5101 tttcgtcccg ttatttgccg ttgagcaggc tgcgaccacg accggaacct ggatgctggc
     5161 acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc cgtaagccag atggcaaagg
     5221 gtatcaattg attatgctgc cgccagagtg ttctccgcca ctggatgatg ccgaaactac
     5281 cgccgcgtgg atgaacaaag tggtagaaaa atgcatcatg atggcaccag agcagtatat
     5341 gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt ccttcacgct attaaatctc
     5401 ccatgccgga tgcttcagaa gggtatccgg cattaccaca gcaaatcccc ctgatttagc
     5461 gataaaagct ctctggattg cgccccctgg aagtcgggcg cataattagt gtgcttatct
     5521 tttcttctta tgttcaccgc gcctggcgca ccaacagcgg attgctatgt caccctgtga
     5581 aaatgacacc cctataaact ggaaacgaaa cctgatcgtc gcctggctag gctgttttct
     5641 taccggtgcc gccttcagcc tggtaatgcc cttcttaccc ctctacgttg agcagcttgg
     5701 cgttacgggc cactccgcct tgaatatgtg gtccggtatt gtcttcagca ttacattttt
     5761 attttcggcc atcgcctcac cgttttgggg tggactcgcc gaccgtaaag gccgaaaact
     5821 catgctatta cgctctgctc tcggcatggg catcgtgatg gtgttgatgg gactggcaca
     5881 aaatatctgg cagtttttga tcctgcgggc gcttcttggt ttacttggcg gatttgtccc
     5941 caacgctaat gctcttatcg ccacacaagt accgcgtaat aaaagcggct gggcactggg
     6001 tacgctctcc acaggcggcg ttagcggtgc gttgctcggc ccaatggctg gcggcctgct
     6061 cgccgatagc tacggcttac gtccggtatt ctttattacc gccagtgtgc tcatactctg
     6121 ctttttcgtc accctgtttt gcatcagaga aaaattccag ccggtcagca aaaaagagat
     6181 gctgcacatg cgggaagtgg tgacatcact taaaaacccg aaactggtac tcagcctgtt
     6241 tgtcaccacg ttaatcatcc aggtggcgac aggctcaatt gcccccattc tgacgctgta
     6301 tgtccgcgaa ctggcgggta acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc
     6361 ggtgccaggc gtggcggctc tgctaagcgc accacgactc ggcaaacttg gcgatcgaat
     6421 cggacccgaa aagatcctga ttacagcgct gatcttttct gtactgctgt tgatcccaat
     6481 gtcttacgtt cagacgccat tgcaacttgg gattttacgt tttttgctcg gtgccgccga
     6541 tggtgcacta ctccccgccg tacagacact gttggtttac aactcgagca accagatcgc
     6601 cgggcgtatc ttcagctata accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt
     6661 gatgggagca gcgatttcag cgaactacgg tttcagagcg gtatttctcg tcaccgctgg
     6721 cgtagtgtta ttcaacgcag tctattcatg gaacagtcta cgtcgtcgtc gaatacccca
     6781 ggtatcgaac tgatttttcg cctttcatac ttgcaaaagc ggagaatcag ctatcctttt
     6841 ccctgaaacc tcatcaactc aaagggagaa tcgtgatgac catgtacgca acgcttgaag
     6901 aagccattga cgctgcacgc gaagaatttc ttgcagacaa ccccggcatc gacgccgaag
     6961 atgcgaatgt gcaacagttc aatgcccaaa aatacgtttt gcaggacggc gacatcatgt
     7021 ggcaagttga gttttttgcc gacgaagggg aagaaggtga atgtttacct atgcttagcg
     7081 gtgaagccgc gcaaagtgtt tttgatggcg actatgatga gatagagata cgccaggagt
     7141 ggcaggaaga gaatacatta catgaatggg acgaggggga atttcagctt gagccaccgc
     7201 tggataccga ggaaggacgc gcagcagctg atgagtggga tgaacgttaa tcactcatac
     7261 gggccatgat gaatgtcgat cggcagcagt aaagtatcaa aaaccagaga gaatggcaga
     7321 tcaaggatcg tgacataacg ccaggcggag tcacgcacat cccattgcac accgggataa
     7381 tattggttgc catgcccctg ccccggtata gtgcgactaa taatgctgcc acagccactc
     7441 agcaaggtca ccatgatgct caccacaatt aaacgcattt gatacctctt tcgtaaaaaa
     7501 ataccggcat aacgccggta tttttattgg ctcgttgttt ttgccggatg cggcgtgaac
     7561 gccttatccg acccacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg
     7621 ataagcgtag cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa
     7681 tgccagtgga ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg
     7741 ggaattccag acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag
     7801 gcgacgatcg cgattcagct tctctggcgt ctcgttcagc gcctgttcaa cgtggcggtc
     7861 acgggcgatt tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag
     7921 agcgttaaat gacgggttaa acactgcgtg cataaagcca tcatcaaggg agcgttgacg
     7981 attcatctca aggaaccgat cggtatcaac cagcacctgc ggcggcgaat actcttccgg
     8041 gatcaggaac agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac
     8101 cgaaacaaac ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg
     8161 cagatccagc cacgccatcc caacagccca cactaaccct aacagcagct gtgagccgtg
     8221 gcgtttgaac gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac
     8281 cacttcccag ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg
     8341 agccagcagc acggaaaaaa gcacttccag cagcaacgat aatgtaacgc gccagaagcc
     8401 gccgtattct ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa
     8461 caacagcacc atagtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca
     8521 caccgggaac aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgtactac
     8581 ctgcaatgca gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat
     8641 cacgcccgtc aggaacaccg cacggtgaac cgggtgcata cccttcacca ggaacagacg
     8701 gaagttcatc aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt
     8761 aggcggtaat tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc
     8821 acggcgcatc aacgccgctt ctacaaagtc atgtgacagg attgaaccgg caaaggaacc
     8881 ttcgcccggc agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc
     8941 gttatgtccc caatagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag
     9001 tggcccatac acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga
     9061 cgctttcggc gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag
     9121 cccgcataaa caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta
     9181 ctggctgccc cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg
     9241 acggcggcgg cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca
     9301 ggctttttgc tctgcgacgc agatatccgg gttataactg tcactaagaa tgtagacatc
     9361 aaagtgtttg gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa
     9421 aacacggttc acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt
     9481 taatggttca tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag
     9541 ttgcaggaag cccattaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc
     9601 aaagaggatc aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca
     9661 caaatcctga ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat
     9721 ggtcttcata taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta
     9781 acggcggatg gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa
     9841 acgagccaga tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac
     9901 cgggttggta cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt
     9961 cagctgatcg cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca
    10021 ggcttgttcc agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt
    10081 gcggtgttcg gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc
    10141 tttctcgctt gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag
    10201 gtaactggta gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca
    10261 tttcagtggt tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg
    10321 taaccgggtt atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt
    10381 gcgctgtgac cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca
    10441 caaaggcgat agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg
    10501 aacgacgcgt ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc
    10561 tgaaggtgat ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg
    10621 tccagtaagc gacgatgtta tcgttggttt catcgttggt tggaatttct accagctcaa
    10681 cgctgccttt accccactcc cctttcggag tcacccatgc gcttggacga agatcgtaac
    10741 gatcatcgag atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt
    10801 gcgggttttc catagagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc
    10861 agatccattc gccattaccg gcatggatag agagaccgtt agagtcgtgc aactccggac
    10921 gatagttatt tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg
    10981 caacccccag tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg
    11041 tgtcacgccc tggcataact acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca
    11101 atgcataaat ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga
    11161 agcgtggaaa ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa
    11221 ggccataaac ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga
    11281 tttcatcgtt tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa
    11341 ggtcttttac cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt
    11401 atttgattcg tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt
    11461 acataccctg atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt
    11521 tgtcatgatt aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg
    11581 gcaagttgct tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga
    11641 catcatcaat actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac
    11701 tcaaccaacg cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc
    11761 tttgtgtgct tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga
    11821 atcccggtat cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca
    11881 ctttccgtgc ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaattt
    11941 tatttatcaa ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc
    12001 ataaaaacct tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc
    12061 taaaggcata acccgacagg gataagacga aaaatccgag attagttaac catatatgaa
    12121 tccagtaccc gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt
    12181 agggatccct tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc
    12241 cgaaccatca ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt
    12301 atttttcgtt atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa
    12361 atggtggaaa gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact
    12421 gacattaccg caatttatta tgctgcaata tgtcaaagga aaagcggaaa gttggcctgg
    12481 gctgtcattg tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt
    12541 tttactggtg ttagtagtca tgacgacgct gtgcgtatgg atatttaagc gcatcagaaa
    12601 taatttagaa aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat
    12661 ttttttatgc ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc
    12721 gcccattctg agtaatggca tgttcaattt tattgtcatg caaacgctgt tttatttgcc
    12781 gttctttatc ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac
    12841 gccgtctcgt ggctgtaccc ttgcagcagc attggcgttt gtcgcttatt tactcaacca
    12901 gcgctatggc agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct
    12961 cggtctgtgg atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc
    13021 agcgcgggtg acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt
    13081 aacgctgttt ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct
    13141 ctgtggcctg atatttgtag tagggattgc gataattctg tatgaaattc atttgcgcat
    13201 cccgttactg aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc
    13261 accagcccgt taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg
    13321 accataaccc gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt
    13381 gcatgacgat caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca
    13441 tcatactggc tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc
    13501 acgaagccca tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata
    13561 aacaccgttt taccatcgat gctaaaggtt ttagcatgca ggctggctcc ggaattaccg
    13621 gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat
    13681 tccacgccat agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca
    13741 acatcgttag cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc
    13801 cgtaagagtt gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg
    13861 cgttcactgg gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt
    13921 gccttgccct cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc
    13981 ggcaatgttc catcaaccag atgatttata aatggactgg attccatttt gcgtaaataa
    14041 cgatgcgtca tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca
    14101 ccctccggga catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg
    14161 gcgaaatcat cggcaacgtc ctctaccacg ggtcctattg ccatgacatc taaatccgaa
    14221 aaaagtggct cctcccctgc tccaaaatag gcatcaccaa tatttcgtcc tcccaccagg
    14281 gtcaccacgc catcgacagt gaaactttta ttgtgcatac ggcgattaag acgggaaaag
    14341 tcggtgatat aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact
    14401 tcaatgcgtg gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg
    14461 tcgtccagca acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac
    14521 agtaaccgac ccgacatatc gtcctgccag atgtaatact gaacatcgag cgtatgctct
    14581 gccatttcgg ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa
    14641 agaccacact gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc
    14701 aaatcattca tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc
    14761 ataacagaca aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt
    14821 tttaactgcg atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc
    14881 aggaaaagcc actgacgtat agctgtttgc cgccaccagt cgtaggctat tgagataggc
    14941 atcctgcaaa agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg
    15001 cactacggct ttagcgggaa gagcgcctgc aagcgtaata acggcatgcc ccgtagggca
    15061 atcgccctgc tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg
    15121 atgaatggcc ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat
    15181 cacatcaacg gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac
    15241 gcctccttac accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt
    15301 ttctgctcag caacgtgcgt tgttcagaaa cttagttata ttcgatgttc attacagcca
    15361 gctttttctg tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttaacat
    15421 taccgctcat taacgctttg ctggaatagg ttttgcccgg aacagaacca ttctgtgggc
    15481 agttcatttc tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc
    15541 cataaaaatg gatcaccccc ccttgcgcgg tgcttcctgc gatggcaata aaaggaaaaa
    15601 gcaaactgcc gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa
    15661 tgattagtca tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag
    15721 accattctct ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag
    15781 agggcggcca ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt
    15841 tcacccgatc ttccggggaa atatttaaac tcaacttcgt caaagcaata ggttgattag
    15901 caggcaatga gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca
    15961 gggacaatat ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg
    16021 tatacacatc cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga
    16081 gtaataacgc attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta
    16141 atacaaatga tgtattagta ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc
    16201 acgttgacgg aggagttaga tgcagtctgg tcaactgcag caccgttgcc accaccgaac
    16261 tgtttaacag tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg
    16321 aagccacgtt gggtcagatc gattgagctg tcatctgagc cctgacctac atctgcacca
    16381 ttaccaccgc catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca
    16441 agtgcagagt taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta
    16501 ttaccgccac caccgtggtt accgccgccg tactgaggaa caacacctgc cagagcgcta
    16561 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
    16621 cggattgatt taaaagtcga atggaaatta acgttgtgtc acgcgaatag ccatttgcga
    16681 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
    16741 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
    16801 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
    16861 ctttgcccgg ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
    16921 ccgtaactga gcactattat tcgtcccagc ttgaccaatt atggctgcct gattaaatga
    16981 agacttactc aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc
    17041 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt
    17101 catgttgtca ccctggacct ggtcgtacat ttaagaaatt aaatcatttc aacttggttg
    17161 ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata
    17221 tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacca aagtattaaa
    17281 acccacgtaa atacgctgtt atctacgcaa aaaatatttt gttttctttt aaatctccgt
    17341 tttccgctat caaaaatcac cagacagtca tttttcttgc ccgtcgctga ttgctgcgat
    17401 atgtcttgcg cacaagccgt gacaaacctc gtctttttgc agtaaaaaat tgtccacgga
    17461 ggtgcggaga a
//
LOCUS       DAZFRN010000100        17385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFRN010000100 DAZFRN010000000
VERSION     DAZFRN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.100"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(32..1315)
                     /gene="gltA"
                     /locus_tag="ACHKUB_003253"
     CDS             complement(32..1315)
                     /gene="gltA"
                     /locus_tag="ACHKUB_003253"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO6656231.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1521..1628)
                     /locus_tag="ACHKUB_003254"
     CDS             complement(1521..1628)
                     /locus_tag="ACHKUB_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656232.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2009..2413
                     /gene="sdhC"
                     /locus_tag="ACHKUB_003255"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2009..2413
                     /gene="sdhC"
                     /locus_tag="ACHKUB_003255"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6656233.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2407..2754
                     /gene="sdhD"
                     /locus_tag="ACHKUB_003256"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2407..2754
                     /gene="sdhD"
                     /locus_tag="ACHKUB_003256"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO6656234.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2754..4520
                     /gene="sdhA"
                     /locus_tag="ACHKUB_003257"
     CDS             2754..4520
                     /gene="sdhA"
                     /locus_tag="ACHKUB_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO6656235.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4536..5252
                     /gene="sdhB"
                     /locus_tag="ACHKUB_003258"
     CDS             4536..5252
                     /gene="sdhB"
                     /locus_tag="ACHKUB_003258"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO6656236.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5453..8254
                     /gene="sucA"
                     /locus_tag="ACHKUB_003259"
     CDS             5453..8254
                     /gene="sucA"
                     /locus_tag="ACHKUB_003259"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO6656237.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8269..9486
                     /gene="odhB"
                     /locus_tag="ACHKUB_003260"
     CDS             8269..9486
                     /gene="odhB"
                     /locus_tag="ACHKUB_003260"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO6656238.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9580..10746
                     /gene="sucC"
                     /locus_tag="ACHKUB_003261"
     CDS             9580..10746
                     /gene="sucC"
                     /locus_tag="ACHKUB_003261"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO6656239.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10746..11615
                     /gene="sucD"
                     /locus_tag="ACHKUB_003262"
     CDS             10746..11615
                     /gene="sucD"
                     /locus_tag="ACHKUB_003262"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO6656240.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11719..12441)
                     /gene="mngR"
                     /locus_tag="ACHKUB_003263"
     CDS             complement(11719..12441)
                     /gene="mngR"
                     /locus_tag="ACHKUB_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO6656241.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12610..14526
                     /gene="mngA"
                     /locus_tag="ACHKUB_003264"
     CDS             12610..14526
                     /gene="mngA"
                     /locus_tag="ACHKUB_003264"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO6656242.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGDTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVRVAEPIRHAEALMQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14544..17177
                     /gene="mngB"
                     /locus_tag="ACHKUB_003265"
     CDS             14544..17177
                     /gene="mngB"
                     /locus_tag="ACHKUB_003265"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO6656243.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEALRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            17202..>17385
                     /locus_tag="ACHKUB_003266"
     CDS             17202..>17385
                     /locus_tag="ACHKUB_003266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656244.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGF"
BASE COUNT         4203 a         4335 c         4727 g         4120 t
ORIGIN      
        1 gttaaaatat ttacaactta gcaatcaacc attaacgctt gatatcgctt ttaaagtcgc
       61 gtttttcata tcctgtatac agctgacgcg gacgggcaat cttcataccg tcactgtgca
      121 tttcgctcca gtgggcgatc cagccaacgg tacgtgccat tgcgaaaatg acggtgaaca
      181 tggaagacgg aatacccatc gctttcagga tgataccaga gtagaaatcg acgttcgggt
      241 acagtttctt ctcgataaag tacgggtcgt tcagcgcgat gttttccagc tccatagcca
      301 cttccagcag gtcatctttg gtgcccagct ctttcagaac ttcatggcag gtttcacgca
      361 ttacggtggc gcgcgggtcg taatttttgt acacgcggtg accgaagccc atcaggcgga
      421 aagaatcatt tttatctttc gcacgacgaa caaattccgg aatgtgttta acggagctaa
      481 tttcttccag cattttcagc gccgcttcgt tagcaccacc gtgcgcaggt ccccacagtg
      541 aagcaatacc tgctgcgata caggcaaacg ggttcgcacc cgaagagcca gcggtacgca
      601 cggtggaggt agaggcgttc tgttcatggt cagcgtgcag gatcagaata cggtccatag
      661 cacgttccag aatcggatta acttcatacg gttcgcacgg cgtggagaac atcatattca
      721 ggaagttacc ggcataggag agatcgttgc gcggataaac aaatggctga ccaatggaat
      781 acttgtaaca catcgcggcc atggtcggca ttttcgacag caggcggaac gcggcaattt
      841 cacgatgacg aggattgttt acatccagcg agtcgtgata gaacgccgcc agcgcgccgg
      901 taataccaca catgactgcc attggatgtg agtcgcgacg gaaagcgtgg aacagacggg
      961 taatctgctc gtggatcatg gtatgacggg tcaccgtagt tttaaattcg tcatactgtt
     1021 cctgagtcgg tttttcacca ttcagcagga tgtaacaaac ttccaggtag ttagaatcgg
     1081 tcgccagctg atcgatcggg aaaccgcggt gcagcaaaat accttcatca ccatcaataa
     1141 aagtaatttt agattcgcag gatgcggttg aagtgaagcc tgggtcaaag gtgaacacac
     1201 cttttgaacc gagagtacgg atatcaataa catcttgacc cagcgtgcct ttcagcacat
     1261 ccagttcaac agctgtgtcc ccgttgaggg tgagttttgc ttttgtatca gccatttaag
     1321 gtctccttag cgccttatta cgtaagactg ccggaactta aatttgcctt cgcacatcaa
     1381 cctggcttta cccgtttgtt atttggctcg ccgctctgtg aaagagggga aaacctgggt
     1441 acagagctct gggcgcttgc aggtaaagga tccattgatg acgaataaat ggcgaatcaa
     1501 gtacttagca atccgaatta ttaaacttgt ctaccactaa taactgtccc gaatgaattg
     1561 gtcaatactt ccacactgtt acataagtta atcttaggtg aaataccgac ttcataactt
     1621 ttacgcatta tatgcttttc ctggtaatgt ttgtaacaac tttgttgaat gattgtcaaa
     1681 ttagatgatt aaaaattaaa taaatgttgt tatcgtgacc tggatcactg ttcaggataa
     1741 aacccgacaa actatatgta ggttaattgt aatgattttg tgaacagcct atactgccgc
     1801 caggtctccg gaacaccctg caatcccgag ccacccagcg ttgtaacgtg tcgttttcgc
     1861 atctggaagc agtgttttgc atgacgcgca gttatagaaa ggacgctgtc tgacccgcaa
     1921 gcagaccgga ggaaggaaat cccgacgtct ccaggtaaca gaaagttaac ctctgtgccc
     1981 gtagtcccca gggaataata agaacagcat gtgggcgtta ttcatgataa gaaatgtgaa
     2041 aaaacaaaga cctgttaatc tggacctaca gaccatccgg ttccccatca cggcgatagc
     2101 gtccattctc catcgcgttt ccggtgtgat cacctttgtt gcagtgggca tcctgctgtg
     2161 gcttctgggt accagcctct cttcccctga aggtttcgag caagcttccg cgattatggg
     2221 cagcttcttc gtcaaattta tcatgtgggg catccttacc gctctggcgt atcacgtcgt
     2281 cgtaggtatt cgccacatga tgatggattt tggctatctg gaagaaacat tcgaagcggg
     2341 taaacgctcc gccaaaatct cctttgttat tactgtcgtg ctttcacttc tcgcaggagt
     2401 cctcgtatgg taagcaacgc ctccgcatta ggacgcaatg gcgtacatga tttcatcctc
     2461 gttcgcgcta ccgctatcgt cctgacgctc tacatcattt atatggtcgg ttttttcgct
     2521 accagtggcg agctgacata tgaagtctgg atcggtttct tcgcctctgc gttcaccaaa
     2581 gtgttcaccc tgctggcgct gttttctatc ttgatccatg cctggatcgg catgtggcag
     2641 gtgttgaccg actacgttaa accgctggcc ttgcgcctga tgctgcaact ggtgattgtc
     2701 gttgctctgg tggtttacgt gatttatgga ttcgttgtgg tgtggggtgt gtgatgaaat
     2761 tgccagtcag agaatttgat gcagttgtga ttggtgccgg tggcgcaggt atgcgcgcgg
     2821 cgctgcaaat ttcccagagc ggtcaaacct gtgcgctgct ctctaaagtc ttcccgaccc
     2881 gttcccatac cgtttctgcg caaggcggca ttaccgttgc gctgggtaat acccatgaag
     2941 ataactggga atggcatatg tacgacaccg taaaaggttc ggactatatc ggtgaccagg
     3001 acgcgattga atatatgtgt aaaaccgggc cggaagcgat tctggaactc gaacacatgg
     3061 gcctgccgtt ctcgcgtctt gatgatggtc gtatctatca acgtccgttt ggcggtcagt
     3121 cgaaaaactt cggcggcgag caggcggcac gcactgcggc agcagctgac cgtaccggtc
     3181 acgcactgtt gcataccctt tatcagcaga acctgaaaaa ccacaccact attttctccg
     3241 agtggtatgc gctggatctg gtgaaaaacc aggatggcgc ggtggtgggt tgtactgcac
     3301 tgtgcatcga aactggtgaa gtggtttatt tcaaagcccg cgctaccgtg ctggcgactg
     3361 gcggggcagg gcgtatttat cagtccacca ctaacgccca cattaacact ggcgacggcg
     3421 tcggcatggc tatccgcgca ggcgtaccgg tgcaggatat ggaaatgtgg cagttccacc
     3481 cgaccggtat tgccggtgcg ggcgtactgg tcaccgaagg ttgccgtggt gaaggcggtt
     3541 atctgctgaa caaacatggc gaacgcttta tggagcgtta tgcgccgaac gccaaagacc
     3601 tggcgggccg tgatgtggtg gcgcgttcca tcatgatcga aatccgtgaa ggtcgcggct
     3661 gtgatggtcc gtgggggcca cacgcgaaac tgaaactcga ccacctgggt aaagaagttc
     3721 tcgaatcccg cctgccgggt atcctggaac tctcccgtac cttcgctcac gttgatccgg
     3781 tgaaagagcc gattccggtt atcccaacct gtcactacat gatgggcggt attccgacca
     3841 aagtgaccgg tcaggcgctg acggtgaacg agaaaggcga agatgtggtt gttccgggac
     3901 tgtttgccgt tggtgaaatc gcttgtgtat cggtacacgg cgctaaccgt ctgggcggca
     3961 actcgctgct ggacctggtg gtctttggtc gtgcagcggg tctgcatctg caagagtcta
     4021 tcgccgaaca gggcgcattg cgcgatgcca gcgagtctga tgttgaagca tctctggatc
     4081 gtctgaaccg ctggaacaat aatcgtaacg gtgaagatcc ggtggcgatt cgtaaagcgc
     4141 tgcaagaatg tatgcagcat aacttctcgg tcttccgtga aggtgatgcg atggcgaaag
     4201 ggcttgagca gttgaaagtg atccgcgagc gtctgaaaaa tgcccgtctg gatgacactt
     4261 ccagcgagtt caacacccag cgcgttgagt gcctggaact ggataacctg atggaaacgg
     4321 cgtatgcaac ggctgtttct gccaacttcc gtaccgaaag ccgtggcgca catagccgct
     4381 tcgacttccc ggatcgtgat gatgaaaact ggctgtgcca ctccctgtat ctgccagagt
     4441 cggaatccat gacgcgccga agcgtcaaca tggaaccgaa actgcgcccg gcattcccgc
     4501 cgaagattcg tacttactaa tgcggagaca ggaaaatgag actcgagttt tcaatttatc
     4561 gctataaccc ggatgttgat gatgctccgc gtatgcagga ttacaccctg gaagcggaag
     4621 aaggtcgcga catgatgctg ctggatgcgc ttatccagct gaaagagaaa gatcccagcc
     4681 tgtcgttccg ccgctcctgc cgtgaaggtg tgtgcggttc cgacggtctg aacatgaacg
     4741 gcaagaatgg tctggcctgt attaccccga tttcggcact caaccagccg ggcaagaaga
     4801 ttgtgattcg cccgctgcca ggtttaccgg tgatccgcga tttggtggta gacatgggac
     4861 aattctatgc gcaatatgag aaaattaagc cttacctgtt gaataatgga caaaatccgc
     4921 cagctcgcga gcatttacag atgccagagc agcgcgaaaa actcgacggg ctgtatgaat
     4981 gtattctctg cgcatgttgt tcaacctctt gtccgtcttt ctggtggaat cccgataagt
     5041 ttatcggccc ggcaggcttg ttagcggcat atcgtttcct gatcgatagc cgtgataccg
     5101 agactgacag ccgcctcgac ggtttgagcg atgcattcag cgtattccgc tgtcacagca
     5161 tcatgaactg cgtcagtgta tgtccgaagg ggctgaaccc gacgcgcgcc atcggccata
     5221 tcaagtcgat gttgttgcaa cgtaatgcgt aattaaatgt tgatgccggg gaaatccccc
     5281 ggctattcag gaaatcttta aaaactgccc ctgacactaa gacagttttt aaaggttcct
     5341 tcgcgagcca ctacgtagac aagagctcgc aagtgaaccc cggcacgcac ataactgtgc
     5401 gtggtagtat ccacggcgaa gtaagcataa aaaagatgct taagggatca cgatgcagaa
     5461 cagcgctttg aaagcctggt tggactcttc ttacctctct ggcgcaaacc agagctggat
     5521 agaacagctc tatgaagact tcttaaccga tcctgactcg gttgacgcta actggcgttc
     5581 gacgttccag cagttacctg gtacgggagt caaaccggat caattccact ctcaaacgcg
     5641 tgaatatttc cgccgcctgg cgaaagacgc ttcacgttac tcttcaacga tctccgaccc
     5701 tgacaccaat gtaaagcagg ttaaagtcct gcagctcatt aacgcatacc gcttccgtgg
     5761 tcaccagcat gcgaatctcg atccgctggg actgtggcag caagataaag tggccgatct
     5821 ggatccgtct ttccacgatc tgaccgaagc agacttccag gagaccttca acgtcggttc
     5881 atttgccagc ggcaaagaaa ccatgaaact cggcgagctg ctggaagccc tcaagcaaac
     5941 ctactgcggc ccgattggtg ccgagtatat gcacattacc agcaccgaag aaaaacgctg
     6001 gatccaacag cgtatcgagt ctggtcgcgc gactttcaat agcgaagaga aaaaacgctt
     6061 cttaagcgaa ctgaccgccg ctgaaggcct tgaacgttac ctcggcgcaa aattccctgg
     6121 cgcaaaacgc ttctcgctgg aaggcggtga cgcgttaatc ccgatgctta aagagatgat
     6181 ccgccacgct ggcaacagcg gcacccggga agtggttctc gggatggcgc accgtggtcg
     6241 tctgaacgtg ttggtgaacg tgctgggtaa aaaaccgcaa gacttgttcg acgagtttgc
     6301 cggtaaacat aaagaacacc tcggcacggg tgacgtgaaa taccacatgg gcttctcgtc
     6361 tgacttccag accgatggcg gcctggtgca cctggcgctg gcgtttaacc cgtctcacct
     6421 tgagattgta agcccggtcg ttatcggctc tgttcgtgcc cgtctggaca gacttgatga
     6481 gccgagcagc aacaaagtgc tgccaattac catccacggt gacgccgcag tgaccggaca
     6541 gggcgtggtt caggaaaccc tgaacatgtc gaaagcgcgt ggttatgaag ttggcggtac
     6601 ggtacgtatc gttatcaaca accaggttgg tttcaccacc tctaatccgc tggatgcccg
     6661 ttctacgccg tactgtactg atatcggcaa gatggttcag gcaccgattt tccacgttaa
     6721 cgcggacgat ccggaagccg ttgcctttgt gacccgtctg gcgctcgatt tccgtaacac
     6781 ctttaaacgt gatgtcttca tcgacctggt atgctaccgc cgtcacggcc acaacgaagc
     6841 cgacgagccg agcgcaaccc agccgctgat gtatcagaaa atcaaaaaac atccgacacc
     6901 gcgcaaaatc tacgctgaca agctggagca ggaaaaagtg gcgacgctgg aagatgccac
     6961 cgagatggtt aacctgtacc gcgatgcgct ggatgctggc gattgcgtgg tagcagagtg
     7021 gcgtccgatg aacatgcact ctttcacctg gtcgccgtac ctcaaccacg aatgggacga
     7081 agagtacccg aacaaagttg agatgaagcg cctgcaggaa ctggctaaac gcatcagcac
     7141 ggtgccggaa gcggttgaaa tgcagtctcg cgttgccaag atttatggcg atcgccaggc
     7201 gatggctgcc ggtgagaaac tgttcgactg gggcggcgcg gaaaacctcg cttacgccac
     7261 gctggttgac gaaggcattc cggttcgcct gtcgggtgaa gactccggtc gcggtacctt
     7321 cttccaccgc cacgcggtga tccacaacca gtctaacggt tccacttaca cgccgctgca
     7381 acacatccat aacggccagg gcgcgttccg tgtttgggac tccgtactgt ctgaagaagc
     7441 agtgctggcg tttgaatacg gttatgccac cgcagaacca cgcaccctga ccatctggga
     7501 agcgcagttc ggtgacttcg ccaacggtgc gcaggtggtt atcgaccagt tcatctcctc
     7561 tggcgagcag aaatggggcc ggatgtgtgg tctggtgatg ttgctgccgc acggttacga
     7621 agggcagggg ccggagcact cctccgcgcg tctggaacgt tatctgcaac tttgtgctga
     7681 gcaaaacatg caggtttgcg taccgtctac cccggcacag gtttaccaca tgctgcgtcg
     7741 tcaggcgctg cgcgggatgc gtcgtccgct ggtcgtgatg tcgccgaaat ccctgctgcg
     7801 tcatccgctg gcggtttcca gcctcgaaga actggcgaac ggcaccttcc tgccagccat
     7861 cggtgaaatc gacgagcttg atccgaaggg cgtgaagcgc gtagtaatgt gttctggtaa
     7921 ggtttattac gacctgctgg aacagcgtcg taagaacaat caacacgatg tcgccattgt
     7981 gcgtatcgag caactctacc cgttcccgca taaagcgatg caggaagtgt tgcagcagtt
     8041 tgctcacgtc aaggattttg tctggtgcca ggaagagccg ctcaaccagg gcgcatggta
     8101 ctgcagccag catcatttcc gtgaagtgat tccgtttggg gcttctctgc gttatgcagg
     8161 ccgcccggcc tccgcctctc cggcggtagg gtatatgtcc gttcaccaga aacagcaaca
     8221 agatctggtt aatgacgcgc tgaacgtcga ataaataaag gatacacaat gagtagcgta
     8281 gatattctgg tccctgacct gcctgaatcc gtagccgatg ccaccgtcgc aacctggcat
     8341 aaaaaacccg gcgacgcagt cgtacgtgat gaagtgctgg tagaaatcga aactgacaaa
     8401 gtggtactgg aagtaccggc atcagcagac ggcattctgg atgcggttct ggaagatgaa
     8461 ggtacaacgg taacgtctcg tcagatcctt ggtcgcctgc gtgaaggcaa cagcgccggt
     8521 aaagaaacca gcgccaaatc tgaagagaaa gcgtccactc cggcgcaacg ccagcaggcg
     8581 tctctggaag agcaaaacaa cgatgcgtta agcccggcga tccgtcgcct gctggctgaa
     8641 cacaatctcg acgccagcgc cattaaaggc accggtgtgg gtggtcgtct gactcgtgaa
     8701 gatgtggaaa aacatctggc gaaagccccg gcgaaagagt ctgctccggc agcggctgat
     8761 ccggcggcgc aaccggctct ggctgcacgt agtgaaaaac gtgtcccgat gactcgcctg
     8821 cgtaagcgtg tggcagagcg tctgctggaa gcgaaaaact ccaccgccat gctgaccacg
     8881 ttcaacgaag tcaacatgaa gccgattatg gatctgcgta agcagtacgg tgaagcgttt
     8941 gaaaaacgcc acggcatccg tctgggcttt atgtccttct acgtgaaagc ggtggttgaa
     9001 gccctgaaac gttacccgga agtgaacgcg tctatcgacg gcgatgacgt ggtttaccac
     9061 aactatttcg acgtcagcat ggcggtttct acgccgcgcg gcctggtgac accggtactg
     9121 cgtgatgtcg ataccctcgg catggcagac atcgagaaga aaatcaaaga gctggcagtc
     9181 aaaggccgtg acggcaagct gaccgttgaa gatctgaccg gtggtaactt caccatcacc
     9241 aacggtggtg tgttcggttc cctgatgtct acgccgatca tcaacccgcc gcagagcgca
     9301 attctgggta tgcacgctat caaagatcgt ccgatggcgg tgaatggtca ggttgagatc
     9361 ctgccgatga tgtacctggc gctgtcctac gatcaccgtc tgatcgatgg tcgcgaatcc
     9421 gtgggcttcc tggtaacgat caaagagttg ctggaagatc cgacgcgtct gctgctggac
     9481 gtgtagtagt ttaagcttca cctgcactgt agaccgacgt ggatagggtg tcggtctacg
     9541 gtttaaaaga taacgattac tgaaggatgg acagaacaca tgaacttaca tgaatatcag
     9601 gcaaaacaac tttttgcccg ctatggctta ccagcaccgg tgggttatgc ctgtactact
     9661 ccgcgcgaag cagaagaagc cgcttcaaaa atcggtgccg gcccgtgggt agtgaaatgt
     9721 caggttcacg ctggtggccg cggtaaagcg ggcggtgtga aagttgtaaa cagcaaagaa
     9781 gacatccgtg cttttgcaga aaactggctc ggcaagcgtc tggtaacgta tcaaacagat
     9841 gccaatggcc aaccggttaa ccagattctg gttgaagcag cgaccgatat cgctaaagag
     9901 ctgtatctcg gtgccgttgt agaccgtagt tcccgtcgtg tggtctttat ggcctccacc
     9961 gaaggcggcg tggaaatcga aaaagtggcg gaagaaactc cgcacctgat ccacaaagtt
    10021 gcgcttgatc cgctgactgg cccgatgccg tatcagggac gcgagctggc gttcaaactg
    10081 ggtctggaag gtaaactggt tcagcagttc accaaaatct tcatgggcct ggcgaccatt
    10141 ttcctggagc gcgacctggc gttgatcgaa atcaacccgc tggtcatcac caaacagggc
    10201 gatctgattt gcctcgacgg caaactgggc gctgacggca acgcactgtt ccgccagcct
    10261 gatctgcgcg aaatgcgtga ccagtcgcag gaagatccgc gtgaagcaca ggctgcacag
    10321 tgggaactga actacgttgc gctggacggt aacatcggtt gtatggttaa cggcgcaggt
    10381 ctggcgatgg gtacgatgga catcgttaaa ctgcacggcg gcgaaccggc taacttcctt
    10441 gacgttggcg gcggcgcaac caaagaacgt gtaaccgaag cgttcaaaat catcctctct
    10501 gacgacaaag tgaaagccgt tctggttaac atcttcggcg gtatcgttcg ttgcgacctg
    10561 atcgctgacg gtatcatcgg cgcggtagca gaagtgggtg ttaacgtacc ggtcgtggta
    10621 cgtctggaag gtaacaacgc cgaactcggc gcgaagaaac tggctgacag cggcctgaat
    10681 attattgcag caaaaggtct gacggatgca gctcagcagg ttgttgccgc agtggagggg
    10741 aaataatgtc cattttaatt gataaaaaca ccaaggttat ctgccagggc tttactggta
    10801 gccaggggac tttccactca gaacaggcca ttgcatacgg cactaaaatg gttggcggcg
    10861 taaccccagg taaaggcggt actacccacc tcggcctgcc ggtgttcaac accgtgcgtg
    10921 aagctgttgc tgctactggc gctaccgctt ctgttatcta cgtaccagca ccgttctgca
    10981 aagactccat tctggaagct attgatgcag gcatcaaact gattatcacc atcactgaag
    11041 gcatcccgac gctggatatg ctgactgtga aagtgaaact ggacgaagcc ggcgttcgta
    11101 tgatcggccc gaactgcccg ggcgttatca ctccgggtga atgcaaaatc ggtatccagc
    11161 cgggtcacat tcacaaaccg ggtaaagtgg gtatcgtttc ccgttccggt acactgacct
    11221 atgaagcggt taaacagacc acggattacg gtttcggtca gtcgacctgt gttggtatcg
    11281 gcggtgaccc gatccctggc tctaacttta tcgacattct cgaaatgttc gaaaaagatc
    11341 cgcagaccga agcgatcgta atgatcggtg agatcggcgg tagcgctgaa gaagaagcgg
    11401 cagcgtacat caaagagcat gttaccaagc cagttgtggg ttacatcgct ggtgtgactg
    11461 cgccgaaagg caaacgtatg ggccacgcgg gtgccatcat tgccggtggg aaagggactg
    11521 cggatgagaa attcgctgct ctggaagccg caggcgtgaa aaccgttcgc agcctggcgg
    11581 atatcggtga agcactgaaa actgttctga aataaatatc tgtaataaga aatagccctc
    11641 gccgcttccc tctacaggaa tggcgaaggg ctgtcggttt cgacatggtt ggccatcgta
    11701 tgatggcctt ttttgtgctt atcgcgatga ttttcgttgc gctatcaggg taaatttata
    11761 gtcatcggta ttaaaagcgt tgcggctata ttcaaacacc cgaccatcaa ctaaatatcc
    11821 acgcgatact ttttcaagaa tcggctttgt ctggctgata ttaagcagac ggctcatctc
    11881 ttcggttggc atcagaggaa tgatttcctg ttcgctacga tcgataacca ttttcttcac
    11941 ttcttcaata aagtgatatt ttgaattttc catgacctgc caggtgagat ccgggaacaa
    12001 cgcaagcggc atccaggttt cttccagcgc cattggcttt tgcttgcgat agcgcacgcg
    12061 cttcacatgc cacacacgat cctgcgcggt gatttgtagc tgttgctgaa gaaaatcgtc
    12121 agccggaatc acttcaaata tcaggacttc actgtgtgta tcgacgtgac ggtccgacag
    12181 tttttcatca aaactggtta actgaaaaat atcgtaattg acccgctctt ctttgacgta
    12241 agtcccgctg ccctgaatgc tttcgaggat ctgttgctcg actaactggc gcaaagcctg
    12301 acgcaccgta acccggctga cgccaaactc tgtttgtaat gccgattcgg taggtaacgc
    12361 atcgccaggt ttaagctcgc cacgcgcaat ttgttcacga atgcgatcgg caatctgccg
    12421 gtataagggc ttgtgtccca tttttagtat ctcattaata cgaatttagc cattatgccc
    12481 gataaattca tcctgtaaat aatacaaata caatacaaat aatttcaatc aagtgaaatt
    12541 gatcacataa tggtattgtt ttatcgggca cactggcgcg actataaaaa cgatcaagtg
    12601 aggatcatga tgaatctgac gactctgacc caccgcgatg cgttgtgtct gaatgcgcgc
    12661 tttaccagcc gtgaagaggc catccacgcg ttgactcaac gtcttgctgc tctggggaaa
    12721 atttccagta ctgagcaatt tctggaagaa gtgtatcgcc gtgaaagcct tggcccgacc
    12781 gccttaggtg aagggttggc tgtgccgcat ggcaaaactg ctgcggtaaa agaagcggcg
    12841 tttgcggtcg ccacactcag cgagccgctt cagtgggaag gcgttgatgg cccggaagcg
    12901 gttgatttag tggtgctgct ggcgattccc cccaatgaag cgggtactac gcatatgcaa
    12961 ctgctgacag cgctgaccac gcgcctggcg gatgatgaga ttcgggcgcg tatacagtcg
    13021 gcgacgacgc ctgatgagtt gctctcggcg ctggatgaca agggagacac gcaaccttct
    13081 gcctcttttt ccaacgcgcc aactatcgtc tgcgtaacgg cctgtccggc gggtattgct
    13141 cacacctata tggctgcgga atatctggaa aaagccggac gcaaactcgg cgtaaatgtt
    13201 tacgttgaaa aacaaggcgc taacggcatt gaagggcgtt taacggcgga tcaactcaat
    13261 agtgcaaccg cctgtatttt tgcggctgaa gtcgccatca aggagagtga gcgttttaat
    13321 ggcattcccg cgctttcagt gcgtgttgcc gagccgattc gccatgcaga agcgttgatg
    13381 caacaagcgc ttaccctcaa gcgtagcgat gagacgcgta ccgtacagca agatacgcaa
    13441 ccggtgaaaa gtgtcaaaac ggagctgaaa caggcactgt tgagcggaat ctcttttgcc
    13501 gtaccgttga ttgtcgcggg gggcacggtg ctggcggtcg cggtattact gtcgcaaatc
    13561 ttcgggctac aagatctgtt taatgaagaa aactcctggc tgtggatgta ccgcaagctg
    13621 ggcggcgggc tgctcggaat tttgatggta ccggtgctcg cggcctatac cgcctattct
    13681 ctggcagata aaccggcgtt agcgccaggc tttgcggctg gacttgccgc caacatgatc
    13741 ggctccgggt ttctcggcgc ggtcgttggc ggattgatag ccggttactt gatgcgctgg
    13801 gtgaaaaatc acttgcgtct tagcagtaaa ttcaatggat tcctgacttt ttatctctac
    13861 ccggtgctcg gtacgttggg agcgggcagt ctgatgctgt ttgtggtggg agaacctgtc
    13921 gcctggatca ataactcgct taccgcctgg ctgaacggtc tgtcaggaag taacgcgctg
    13981 ttgctgggtg ccattctcgg ttttatgtgt tcctttgacc ttggagggcc agtgaacaaa
    14041 gccgcttatg cattctgcct gggcgcaatg gcgaacggcg tttacggccc gtatgccatt
    14101 ttcgcctccg tcaaaatggt ttcggcattt accgtaaccg cttccacgat gctcgcaccg
    14161 cgcctgttta aagagtttga aattgagacc gggaaatcca cctggctgtt agggctggca
    14221 ggtattaccg aaggggcgat cccgatggcg attgaagatc cgctgcgggt tattggttcg
    14281 tttgtgctgg gctctatggt aacgggcgct attgtcggtg cgatgaatat cggcctttcg
    14341 acacccggtg ccggcatttt ctcgctcttt ttacttcatg ataatggcgc gggcggtgtt
    14401 atggcggcaa ttggctggtt tggtgcggca ttggtggggg ctgcaatctc gactgcaatt
    14461 ctcctgatct ggcggcgtca cgcggttaag catggcaact atctgactga tggcgtaatg
    14521 ccataaacaa aaaggaaacg acgatgaaag cggtatctcg cgttcacatc accccgcata
    14581 tgcattggga tcgggagtgg tatttcacca ccgaagagtc acgcattctg ctggtcaata
    14641 atatggaaga gatcctgtgc cgactggaac aggacaacga atacaaatat tacgtactcg
    14701 acgggcaaac ggcgatcctc gaagattatt tcgcggtgaa accggaaaac aaagaccgcg
    14761 tgaagaaaca ggtagaagcc ggtaagttga ttatcggccc ctggtatacc cagaccgata
    14821 ccacgattgt ttctgcggaa tccatcgtcc gtaatctgat gtacggaatg cgtgactgcc
    14881 tcgcgtttgg cgagccgatg aaaataggtt atttaccgga ttcctttggc atgtccgggc
    14941 aactgccgca tatctacaat ggatttggca ttacccgcac tatgttctgg cgcggatgtt
    15001 cggagcgcca cggtactgat aaaaccgagt ttttatggca aagcagtgac ggtagcgaag
    15061 tgacggcgca ggtgctgccg ttgggttatg ccatcggtaa gtacttacct gccgacgaaa
    15121 acggattacg caaacgcctc gacagttatt ttgacgtgct ggaaaaagcg tctgtaacca
    15181 aagagatttt attgccgaat ggtcatgacc agatgccatt gcagcaaaat atcttcgaag
    15241 tgatggagaa acttcgcgaa atttatcctc aacgaaagtt tgtgatgagc cgctttgaag
    15301 aggtattcga gcagatcgaa gcgctgcgtg aaagtctggc caccctgaaa ggtgaattta
    15361 tcgatggcaa atatatgcgc gtacatcgca ccatcggttc cacgcgcatg gatatcaaaa
    15421 ttgcccacgc acgtattgaa aataagattg ttaatctgct ggaaccgctg gcaacgctgg
    15481 cctggacgtt gggttttgaa taccaccacg gcttgctgga gaaaatgtgg aaagagatct
    15541 taaaaaatca tgcccacgac agtatcggtt gttgttgcag tgacaaagtt catcgcgaaa
    15601 ttgtcgcccg cttcgaactg gctgaagaca tggcggataa tctgctgcgt ttctacatgc
    15661 gcaaaattgc cgataacatg ccacagagtg atgccgacaa actcgtgctg tttaacctga
    15721 tgccctggcc gcgggaagaa gttattaaca ccactgtgcg gctgcgcgcc agccagttta
    15781 atctacgaga cgatcgcggt cagcctgtac cgtattttat tcgccatgcc cgtgagatcg
    15841 atccaggcct aatcgatcgg caaatagttc attacggtaa ttacgatccc tttatggagt
    15901 ttgatataca gatcaaccag attgtccctt ctatgggcta tcgcacgctt tatatcgaag
    15961 cgaatcagcc tggcaacgtg attgcggcaa aaagtgacgc tgaagggata ctggaaaatg
    16021 ctttctggca aattgcgctc aatgaggatg gaacgctaca actggtagat aaagatagcg
    16081 gtgtgcgcta tgaccgggtg ttgcaaattg aagaaagctc tgatgatggt gatgaatatg
    16141 actattcacc cgcaaaagaa gagtgggtaa tgacttctgc aacagcgaaa ccgcaatgcg
    16201 agattactca tgaggcctgg cagagcaggg ctgttatccg ctatgacatg gcggtgccgc
    16261 tcaatttgtt ggaacgaagc gtccggcaat ccacgggcag agtaggggtg gagatggttg
    16321 tcactctgag tcataacagc agacgtattg atgtggatat caaccttgat aaccaggctg
    16381 acgatcatcg ccttcgcgtc ctgatcccta catcttttaa caccgatagt gttctggcag
    16441 atacccagtt tggttcgcta acgcgccccg tgaacgacag tgcaatgaac aactggcagc
    16501 aagaaggctg gaaagaagcg ccggttccgg tatggaatat gctcaactat gcagccttac
    16561 aggaagggcg taacggcatg gctgtcttta gcgaagggct acgtgaattt gaagtcatcg
    16621 gtgaagagaa gaaaaccttt gccattacgt tgctgcgtgg cgtgggctta ctgggcaaag
    16681 aagatctgtt tttaaggcct gggcggcctt cgggaattaa aatgccagtc ccggactcac
    16741 aactacgtgg tctgctttct tgtcgcctaa gtttattgag ttataccggt acgccaaccg
    16801 ccgctggtgt agctcagcag gcgcgagcat ggctgactcc agtacagtgt tacaacaaaa
    16861 tcccatggga tgcgatgaag ctcaacaaag ccggattcaa cgtgccggaa agttatagtt
    16921 tgttgaaaat gcccccagtg ggatgcctga taagcgcact taagaaagct gaagaccgac
    16981 aagaagtgat tttacggctg tttaatccgg ctgaatcagc aacctgtgat gcgactgttg
    17041 ctttcagtcg cgaggtgatt tcttgctcag aaacgatgat ggatgaacac attaccaccg
    17101 aggaaaatca aggttcaaat ctatcggggc cttttttacc cggccagtca cggacgttca
    17161 gttaccggct tgcctgaata gcaatcaaac cgaagccaca tatgcgcggc cagattgttg
    17221 acaaagggcg ctttgttcat gccggatacg gcatgaacgc tttattcggt ctacaaaagc
    17281 aggcaaattc aatatattgc agagatgatg tagacactgg caagcgtagc gcatcaggta
    17341 attttgcgtt tatcttcact ctcaagccac gtatatgtgg cttta
//
LOCUS       DAZFRN010000101        17148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFRN010000101 DAZFRN010000000
VERSION     DAZFRN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.102"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            292..1239
                     /gene="stxA1c"
                     /locus_tag="ACHKUB_003267"
     CDS             292..1239
                     /gene="stxA1c"
                     /locus_tag="ACHKUB_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1c subunit A"
                     /protein_id="HFO6656245.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSVNAILGSVALILNCHHHASRVARIVP
                     NEFPSMCPVDGRVRGITHNKILWDSSTLGAILIRRAISS"
     gene            1250..1519
                     /gene="stxB1c"
                     /locus_tag="ACHKUB_003268"
     CDS             1250..1519
                     /gene="stxB1c"
                     /locus_tag="ACHKUB_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1c subunit B"
                     /protein_id="HFO6656246.1"
                     /translation="MKKILLIAASLSFFSASVLAAPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            2299..4209
                     /locus_tag="ACHKUB_003269"
     CDS             2299..4209
                     /locus_tag="ACHKUB_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6656247.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLAD
                     MAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAIL
                     SCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVD
                     DSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKY
                     AFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHT
                     LAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDT
                     VEVKANKAGAAA"
     gene            4349..4531
                     /locus_tag="ACHKUB_003270"
     CDS             4349..4531
                     /locus_tag="ACHKUB_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6656248.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            4557..4829
                     /locus_tag="ACHKUB_003271"
     CDS             4557..4829
                     /locus_tag="ACHKUB_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6656249.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            4906..5121
                     /locus_tag="ACHKUB_003272"
     CDS             4906..5121
                     /locus_tag="ACHKUB_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6656250.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            5126..5659
                     /locus_tag="ACHKUB_003273"
     CDS             5126..5659
                     /locus_tag="ACHKUB_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6656251.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATQVDGKPVVPGMKLSKEKCDQVNAIERDKVLAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            5950..6081
                     /locus_tag="ACHKUB_003274"
     CDS             5950..6081
                     /locus_tag="ACHKUB_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656252.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            6045..6199
                     /locus_tag="ACHKUB_003275"
                     /pseudo
     CDS             6045..6199
                     /locus_tag="ACHKUB_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6264..6725
                     /locus_tag="ACHKUB_003276"
     CDS             6264..6725
                     /locus_tag="ACHKUB_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6656253.1"
                     /translation="MNRILYVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREAPTTSGVDNATAPRLADTVERDYFTLRERLMLMQKQLEGAQDYIRTQC"
     gene            7025..7831
                     /locus_tag="ACHKUB_003277"
     CDS             7025..7831
                     /locus_tag="ACHKUB_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6656254.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            7812..9518
                     /locus_tag="ACHKUB_003278"
     CDS             7812..9518
                     /locus_tag="ACHKUB_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6656255.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQRKEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            9518..11662
                     /locus_tag="ACHKUB_003279"
     CDS             9518..11662
                     /locus_tag="ACHKUB_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO6656256.1"
                     /translation="MKNEINTTVMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            11820..12827
                     /locus_tag="ACHKUB_003280"
     CDS             11820..12827
                     /locus_tag="ACHKUB_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656257.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGIGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            12851..14065
                     /locus_tag="ACHKUB_003281"
     CDS             12851..14065
                     /locus_tag="ACHKUB_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO6656258.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            14121..14510
                     /locus_tag="ACHKUB_003282"
     CDS             14121..14510
                     /locus_tag="ACHKUB_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656259.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            14560..15021
                     /locus_tag="ACHKUB_003283"
     CDS             14560..15021
                     /locus_tag="ACHKUB_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656260.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            15005..15568
                     /locus_tag="ACHKUB_003284"
     CDS             15005..15568
                     /locus_tag="ACHKUB_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656261.1"
                     /translation="MAELSDFLPYVRRQISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            15568..16218
                     /locus_tag="ACHKUB_003285"
     CDS             15568..16218
                     /locus_tag="ACHKUB_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO6656262.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLSCVPGARQTLPDGAIQLLDVICLSDGSAIKPVSREVLDAQYPDWHMLRG
                     KPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            16215..>17148
                     /locus_tag="ACHKUB_003286"
     CDS             16215..>17148
                     /locus_tag="ACHKUB_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO6656263.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAWNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPQGETGPKGDVGPKGETGPVGPQGPAGPKGER
                     GDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGD"
BASE COUNT         4409 a         3832 c         4823 g         4084 t
ORIGIN      
        1 aagtttgcgt aacagcattt tgctctacga gtttgccagc ctcccccagt ggctggcttt
       61 ttttatgtcc gtaacatcct gtgtatcaat aaatgttgtt gtctacgtac gtcaagtagt
      121 cgcatgagat ctgaccagat atgttaaggt tgcagctctc tttgaatatg attatcattt
      181 tcattacgtt attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt cagggcgtgg
      241 aggatgtcaa gaatatagtt atcgtatggc gctcaaggag tattgtgtaa tatgaaaata
      301 ataattttta gagtgctaac ttttttcttt gttatctttt ctgttaatgt ggttgcgaag
      361 gaatttacct tagatttctc gacagcaaag acgtatgtag attcgctgaa tgtcattcgc
      421 tctgcaatag gtactccatt acagactatt tcatcaggag gtacgtcttt actgatgatt
      481 gatagtggca caggggataa tttgtttgca gttgatgtca gagggataga tccagaggaa
      541 gggcggttta ataatctacg gcttattgtt gaacgaaata atttatatgt gacaggattt
      601 gttaacagga caaataatgt tttttatcgc tttgctgatt tttcacatgt tacctttcct
      661 ggtacaactg cggttacatt gtctggtgac agtagctata ccacgttaca gcgtgttgcg
      721 gggatcagtc gtacggggat gcagataaat cgccattcgt tgactacttc ttatctggat
      781 ttaatgtcgc atagcggaac ctcactgacg cagtctgtgg caagagcgat gttacggttt
      841 gttactgtga cagctgaagc tttacgtttt cggcaaattc agaggggatt tcgtacaaca
      901 cttgatgatc tcagtgggcg ttcttatgta atgactgctg aagatgttga tcttacgttg
      961 aactggggaa ggttgagtag tgtcctgcct gactatcatg gacaagactc tgttcgtgtt
     1021 ggaagaattt cttttggaag tgttaatgca attctgggta gcgtggcatt aatactgaat
     1081 tgtcatcatc atgcatcgcg agttgccaga attgtaccta atgagtttcc ttctatgtgc
     1141 ccggtagatg gaagagtgcg tgggattacg cacaataaaa tattgtggga ctcatccact
     1201 ctgggggcaa ttttgatacg cagggctatt agcagttgag gggggtaaaa tgaaaaaaat
     1261 attattaata gctgcatcac tttcattttt ttcagcaagt gtgctggctg cgccagattg
     1321 tgtaactggg aaggtggagt atacaaaata taatgatgac gataccttta cagttaaagt
     1381 gggagataaa gaattattta ctaacagatg gaatcttcag tctcttcttc tcagtgcaca
     1441 aattactggg atgacggtaa ccattaaaac taatgcctgt cataatggag ggggattcag
     1501 cgaggttatt ttccgttgac tcagaatagc ccggtgaaga gaacaggcgg agatttatca
     1561 atgttaaata catctcaatt cagtcagttg attgttgcct gtctgataat agatgtgcta
     1621 caaatttctg catggtgaat ccccctgagc ggcggggcat atcagcgtca caggtgtttc
     1681 tgttttacct ctatcctttc tgtgcgggtt caggtgctga tactgaactc accgggaggc
     1741 acccggcacc atgcaatgaa cggtacatag cgctactctc cagcccctct ccggaggggc
     1801 tttttaatga ggggaagaca ctgggcagtg aaatgttaaa tcactcacaa ttcaggtagt
     1861 tgacggttgt ctgcttggct gcgaatttgt taaaaaaatc ctgcatggtg aatccccctg
     1921 tgcggagggg cgactggtga atggtatgat ctctttgatg atcgtaagcg agaatacgcg
     1981 ggtttggtgg caccaggccg aactcaccgg gaggcacccg gcaccatgca gtatacagag
     2041 attaggcata tatcagggcc cctcatagca ggggcctttt tacatgcaaa aaaaagccgc
     2101 cccgggaaga gcggttggca agaagaaaag caatatgaac aattaattaa cgctgcgaat
     2161 aataccttac agtaatcacc ctgcgcaact gtgaggagtg tatttctttt ttgcgggtgg
     2221 ttgtcttttt cccttgtgtt tcgggacttc cgctcactcc ttatcttatt cagtacatta
     2281 tcccggccgg gaggattcat ggcatttaaa cactatgacg tggtcagggc ggcgtcgccg
     2341 tcagaccttg cggaacgaat aactcaaaaa ctgaaggaag ggtggcagcc ttatggtagt
     2401 gcgctgattt cgacagctgg ttatggtgcg gagttcatcc agccagttgt gagtgagggg
     2461 agcatctcat caccagagga gccaggcaac cgtccgacga cctcagcgcc ttctgttgcg
     2521 ccagaatatt actatgtgat cgcgcttgct ggtcagtcca atggtatgtc atacggtgag
     2581 ggactgccat taccggatac attcgacagc cctgatccac gtattaaaca gttagcgcgt
     2641 cgcagtacgg tgacaccggg aggtgctgca tgtatctata acgatattat cccggcagat
     2701 cactgtctcc atgatgtgga ggatatgaca gggcttaatc atcccaaagc ggatttatcg
     2761 aaaggtcagt acggctgtgt gggacagggg ctgcatatcg ccaaaaagct tctgccattt
     2821 ataccggcga atgcgggcat tcttcttgtt ccgtgctgcc gtggtggttc agctttcacc
     2881 accggaaccg atggcacata cagtgacact actggcgcct cagagagttc cacccgctgg
     2941 ggggtgggca ggccgctgta taaggacctc attggtcgta caaaagccgc gctggcgaag
     3001 aatccgaaaa atgtgctgct tgccgtggta tggatgcagg gggaatttga ttttaacgga
     3061 acgccagcga atcatgcagc ccgttttaca gaagtggtgg atcaataccg tgcggacctt
     3121 gcagatatgg cggggcagtg tgtgggtggc tcagctgaca gtgttccctg gatttgtgga
     3181 gacacaacat atttctggaa gcagaagaac gaaacggcgt accagacggt gtatggtagt
     3241 tacaaaaata aaacggaaaa gaatatccat ttcgtgccgt tcatgaccga tgagaacggg
     3301 gtgaatgtgc cgacgaacaa accggaagaa gacccggata ttccggatat cgggtattac
     3361 ggttcaaaat ggcgaacgga cagaagcacc tggacatctc aggacagggc cagtcatttc
     3421 agttcatggg ctcgccgtgg gattatttcc gaccgtctgg caacggcgat tctgagctgc
     3481 gcgggtaagt cttctgcgtt tgttaatggt actgccgggg tggttgttcc agacagaccg
     3541 gttaccacct cagagtctgt aattttttac gatgccaaaa aagcttcaga caatcagctg
     3601 aaaccttatg gctgggacgg tatggatggc agacgcacac tggttgatga cagcggcaat
     3661 aaagctctgc gaattgagaa aaataacagc gcgaaatcct ggtcaatgta ctgtgatatt
     3721 gctgcagaca aggcaaaact tttactggaa aaaggcgggg aaattgctgt ccggtttaaa
     3781 atccccgaaa acgtcaatct tgagacaacc agaaacaagt atgcctttgg tttgtactgg
     3841 cgaatagcgg aatggccggg tgagggtggt gaaggctatc tgagttcttt ctttgtccag
     3901 acagataaag ccagtattga tgttgcatac catcatacag ttaatcaaca aaaagaactt
     3961 ggcacgtttg gcgcattcga ccatgactgg catacgcttg catttaaatt taagggcagt
     4021 aacagcatta atgttactcc ggtgcttgat ggtgtggatg gacaggcgtt tgacctggtg
     4081 aaatgggcca atactgctaa tggactcaac aggtttgtca ttacggatat tacaggtagt
     4141 gcagaaacct accctgtact tattgatacg gtggaagtta aagcaaacaa agctggagca
     4201 gccgcataat tgctaaaaaa agccgccagc ggcaggaatg gaagctggcg gaggtaatcc
     4261 caatggagaa tgtaaagaaa agatgctttc gtacattggt tttttaaatg aaaacagttc
     4321 tcattgtcaa ccataacggt aagaaattat gatatttatt catcaggtga tgctgtactt
     4381 ctgtacggcg gtctgtgtgc tgtatcttct ttcgggtggg tacagggctg tgcgcgattt
     4441 ctggtgcagg cagattgata aaagggccgc tgagaaaatc agcgccagtc agtcagccgg
     4501 aagcaaaccc gaagagcccg ttactcctta acaacccctt tcagcgagaa aatcccatgt
     4561 cagaaatcac atccctggtc actgcagagg cagtgaagga agtcctgcgc tctgaagaag
     4621 tccggagcgc actgaaacag aaacttcgcc agaatcttga ggcgcgtctt gatgcagaag
     4681 ttgatgccat tctggatgag ctgcttggtg tacaggcaga gccaccgact gaagcgggag
     4741 ataccaccgc agagagcggt gaagttcagc ctgaatcacc ggtcgccgat gcgactgaac
     4801 ctcaacccga atcggtcatg atgctgtaac ggggagtcag ggccatcagt aaacagctgc
     4861 tggccttttt catgttgtga gcttccggat tgcgggagac ggggtatgta ccagatggaa
     4921 aaaatcacaa caggtgtgtc atacaccacg tcagcggtgg gaacgggcta ctggttcctg
     4981 cagttgctgg acagggtttc cccgtctcag tgggcggcaa taggcgtgct ggggagtctg
     5041 ctgtttgggc tgctgacata tctgacgaac ctgtatttca aaatcagaga ggaccggcgt
     5101 aaggcggcgc ggggagagta agctgatgag caggaaattc cgctatggtt tatcagcggt
     5161 cgttttggcg ctgattgctg caggtgcttc tgcgcctgaa atcctcgacc agtttcttga
     5221 cgaaaaagaa ggtaaccaca ccacggcata ccgtgatggt gcgggtatct ggaccatctg
     5281 ccgtggtgcc acccaggtgg atggtaagcc tgtcgtccct ggcatgaagt tgtcgaagga
     5341 aaaatgcgac caggttaacg ccattgaacg tgataaggtg ctggcatggg tggcgaaaaa
     5401 catcagagtg ccactgaccg aacctcagaa agcggggatc gcgtcattct gtccttacaa
     5461 cattggcccc ggtaagtgtt tcccgtcgac gttttataaa cgaattaatg caggcgatcg
     5521 caggggagcg tgtgaagcga ttcgctggtg gattaaggac ggtggcagag actgccgtat
     5581 ccgctcaaat aattgctacg gtcaggtatc ccgtcgcgac caggagagtg cgctggcatg
     5641 ttggggtatc gacaggtagc agaatatttt gctgaaaaat aacgttggtc aacgcggacg
     5701 gataacacga aatcctgcga actggcaaaa cctaagtgaa taaaagtaaa aaccccgttt
     5761 ttggcagcaa gcggggtttt gtgtttatgg cagtaagcta tgggtggctg ccttgattga
     5821 ttttagcaaa ctgattaggg agttgcgact catgattagt caattaccaa actggaagtt
     5881 tttgctggtc tggagcatcc cttttttatg gggggtatcc cagttaattg tggcaattaa
     5941 ggggtagcta tgtcagacaa actcataacg ctggcgaaga tcctctgtgt aatcgtcggc
     6001 atttcatttt cactaatgct ggttgctatt tttcttttcc tgccctggat gatgttgtct
     6061 tcgtcgggga tgctggggta acagtgactg atgacatcag cagagcgctg gctttgctat
     6121 taagtgggtg gcggttggta tcgctgtgtc tccgatactg tatgggctgg caaaactggt
     6181 cattgcgctg aaatcgtgaa ctttatttaa acagatgagt aatgaaattg ctgtactggt
     6241 ccgggcaatt cgctggtggt gatatgaacc gtattctgta tgtggtgatt attgtactgc
     6301 tggtggcctg tggtgcgctt agtctagggc tgaatcatta ccgcgataac gccatcacct
     6361 acaaagcgca gcgcgataaa aaagccagag agctggagct agcaaacgca accattactg
     6421 acatgcagca gcgccagcgt gatgttgctg cgcttgatgc cagatactca agggaattag
     6481 ccgatgcgag agctgaaaat gaaactctgc gcgctgatgt tgccgctggt cgtaagcgcc
     6541 tgcggatcaa cgccacctgc tccggtaccg tgcgtgaagc ccccaccacc tccggcgtgg
     6601 ataatgcaac cgccccccga ctggcagaca cagttgaacg ggattatttc actctcagag
     6661 agcggttgat gctgatgcag aagcagctgg aaggggcaca ggactatatc cgcactcagt
     6721 gctgaactaa gttttgctga tgcgccgtat cgtcgccgta ttcccgcata aacagagacc
     6781 gcagccacct tatctgcgtg agtgtgcggg ggtaatcaaa aacgatgcac accgggtttt
     6841 ctcatttttc acgagatggg agcgatttcc cgcgaagccg cctgtccggt gcggtggtgg
     6901 aagaaaccgg ataaaacaac ctcattgtac aaatatcgat caagtatggc gctgctgtgt
     6961 gaattctgaa aaatcacagc ggtcattatg cgtcagtttt taacagagga cgtcagaaag
     7021 tgacatggca aagctggact ggaaaaagct ggagcaggca ttccgacgcg aacatgccga
     7081 aacgggaata acattactgg actggtgccg gaagaaaaag attaattaca acaccgccag
     7141 aacccgtata aaaatgggca aaatcgatca tgaaattgat cataaaaccg atcatgaaat
     7201 cgatcatgac atctcagatg aagaacccag caatgacgcg tgttccgacg atgaaaaatg
     7261 cgcaaaaaac tctgaaaaaa actgcgcaaa ttcggcagaa acgaaacgga ttcgtggttc
     7321 ccgactttta cctccttcaa acgctttttc tcagcgaaac acccacgccg taagacaccg
     7381 tggatatgcg aagtatcttg aggcagataa cctcatggat gatgcgtccg acatggtgct
     7441 gttcgatgaa ctggtgttca cccgggcccg cgcactttca gtaactaagg cacttaaagg
     7501 gatgttcgcc gacctggaag aggcaactga cgtggaaacc cgcgttgctc tttacgacaa
     7561 aatactcaaa gctgaacagg cccttgaccg gaatattgcc cgtatcgagt caattgaacg
     7621 ctcattgctg acgctggacg tcctggctga gacagcacca aaacttcgtg ctgaccggga
     7681 aagaatcaac gccgccagag ataaactcag agcggaaacc gatattctga ccagccagcg
     7741 ccggggtgtt attacgccag tcagtgacat cgtgtcatcg ctgcatgaaa tgagtaattc
     7801 ggggagactg gatgacattc cggaagaata aaacgcgatg tgatgaacct gcagaaatga
     7861 ctgagaccga acaacgtctg ttcattatga caaaactgag caatccctgg tggcgcctca
     7921 atcatctcta caaaatacag aacgaaaaag gtgaactggt caccttcaga atgcgaccgg
     7981 cgcaacgtca gttgtttcgt aacatgcata ataaaaacat tatcctgaaa gcgcgccagc
     8041 tgggattttc cacagccatt gatatttatc ttctcgacca ggcattattc attccgcatc
     8101 tcaaatgcgg gatcgtcgct caggataaac aggctgccag tgaaattttc cgcacaaaaa
     8161 ttgctgtacc gtttgatcat ctccctgact ggctgagagc ctcattcacc atcgttgaac
     8221 gtcgtagcgg tgccagcggt ggctatatcc tgtttggtca cggctcgagt attcaggtgg
     8281 caacctcatt tcgctcaggt acggtgcagc gcctgcatat ctcagagcac ggcaaaattt
     8341 gcgcgaaata tccggctaag gcgaaagagc tgcgaaccgg tacgcttaat gccgtctctg
     8401 atgaatgcat tatttttgat gaatccacgg ctgaaggcgt gggtggtgat ttttacgaga
     8461 tgagtaaccg tgcacaggag agcactgcat caggcttatt gctgacggca caggattata
     8521 aattccattt ttacgcatgg tggcaggatc ctaaatacag cgccagagtg cctgaaagcg
     8581 ggctgaagct gtcacgggaa aaaatgacgt atttttctgc ggttgagaag gcaatgaaca
     8641 tcacgcttac cgatgagcag aagcagtggt acatcagtaa ggaaactgaa cagcgtaagg
     8701 aaatgaagca ggagtttccc tcaacgccac aggaggcgtt tctgacgtcc ggacgacgtg
     8761 tgttcagtgc cgaaagtaca ttgcaggcag aatcattctg ttcgccaccg atgattgttt
     8821 atgacattga acctgttaca ggagcgaaga ctaaagctca gtctctgcgt gaaggaaata
     8881 aaaacgagtt gcagcggacg ctgatgaatt atctgctggt atgggaactg ccggatccgg
     8941 atgaagagta tgtttgtggg gcagatactg ccgaagggct ggagcacgga gaccgctcat
     9001 cgctggatgt tgtcaaacgc agtaatggcg agcaggtggc tcactggttc gggcatctcg
     9061 atgctgaact ttttgctcat ctcatttcgc aggtctgtcg tatgtataac aacgcgtttg
     9121 tggggccgga gcgtaataat cacggacatg cagttatcct gaaactccgg gaactctatc
     9181 cgacacgtta tatctacaac gaacagcatc ttgaccaggc atatgacgac gatacgcccc
     9241 gccttggctg gctgacaacc cgtcagagca aacctgttct gaccgaagga atgaaaacgc
     9301 tcctgaataa tggaatatca ggtatccgct ggtcaggcac attatcggaa atgaacacct
     9361 acgtttatga cgcgaaaggc tccatgaatg cacaggaagg ctgctttgat gatcagctca
     9421 tgagctacat gattgcccag gagatgcgcg ccagaatgcc ggtgagggta aaacagaaaa
     9481 cggataaacg cagaaccaca cactggatgg cacactgatg aaaaatgaaa ttaacaccac
     9541 agtgatgaaa aacgatcatg gatccacgcc gcgtttttct cagcgtcagt tactgtctct
     9601 ctgttctgat attgacagtc agcctctctg gcgtgatgca gcaaacaagg cctgtgcgta
     9661 ttatgatggc gaccagctgg caccggaagt tatccaggta ctgaaagatc gcggtcagcc
     9721 catgaccatc cataacctca ttgcccccac ggtagatggt gtactgggaa tggaggcaaa
     9781 aacaagaacg gacctgatag tgatgtcaga cgatccgaac gatgaaacag agaaactggc
     9841 agaggccatt aatgcggagt ttgctgatgc gtgccgtctt ggcaacatga ataaggcccg
     9901 ctccgatgcc tatgcggaac aaatcaaggc ggggctcagt tgggtggagg tcaggcgcaa
     9961 cagcgatccg ttcggacctg aattcagggt atccacagtc agcagaaatg aggttttctg
    10021 ggactggctc agccgggaat ccgacctgag tgactgccgg tggctgatgc gtcgccgctg
    10081 gatggatacc gatgaggcaa aagccacgtt cccgggaatg gctcaggtta tcgattatgc
    10141 tattgatgac tggcgtggtt ttgtcgatac cacgattact gaaggccagc ccagtccgtt
    10201 gatgagtgca tgggaagagt atcagtcatg ggatcgcagg gagaatgaat ggcttcagcg
    10261 tgaacgccgt cgtgtgctgc ttcaggtggt ttattaccgt acattcgagc gtcttccggt
    10321 gattgaactc agtaatggac gggtggtggc ttttgataaa aataatctga tgcaggcggt
    10381 ggctgtggca tccgggcggg ttcaggtgaa agtcgggcgg gtaagtcgta ttcgtgaagc
    10441 ctggtttgtg ggcccacact ttattgtgga tcgcccctgt agtgcaccgc aggggatgtt
    10501 tccgctggtt cctttctggg gataccgaaa ggataaaacc ggggagccat acgggttaat
    10561 ttcccgcgcc attccggcac aggatgaggt gaattttcgt cgtatcaagc tgacctggct
    10621 gcttcaggcc aaacgcgtga ttatggacga ggatgccacc cagttgtcag acagcgacct
    10681 gatggagcag atcgaacgtc cggatggcat tattaaactg aatccggccc gaaaaaatca
    10741 gaaaagtgtc gcagatgttt ttcgggttga gcaggatttt caggttgcca gccagcagtt
    10801 tcaggtcatg caggaatcgg aaaaacttat ccaggatacc atgggagtgt attccgcatt
    10861 tctcgggcag gattcaggtg cgacgtcagg cgtggctatc agtaacctgg tggagcaggg
    10921 ggccacaacc cttgcggaaa tcaacgataa ctaccagttt gcctgccagc aggtgggaag
    10981 actgttgctg gcttatcttc tcgatgacct gaaaaaacgc cgtaatcatg cagtggtgat
    11041 taatcgcgat gatcgccagc gtcgccagac cattgtcctc aatgctgaag gtgataatgg
    11101 tgaactgacc aatgatattt caaggttaaa tacacatatt gcgctggcgc ctgttcagca
    11161 gacaccggcg tttaaggcac agcttgcaca gagaatgtca gaggttattc aggggctgcc
    11221 gcctcaggtg caggctgttg tgctcgacct gtgggttaat cttctggatg tgccgcagaa
    11281 acaggagttt gttgagcgta ttcgtgctgc gctggggacg ccaaaatcac cggatgaaat
    11341 gacgccggaa gaacaggaag tagcggcaca acaacaggca cttcagcaac aacaggcaga
    11401 actccagatg cgcgagatgg ctggcagagt ggcaaaactg gaagctgacg ccgccagggc
    11461 acatgcagct gcacaacggg ataatgccag tgcacagcgg gaagtcgccc tgacacaggg
    11521 gcagcgttat gtggatgcgc ttaaccaggc acatacggca gaaatcatta ccggcgtaca
    11581 gaatatggaa caggagcagg acgttcttca gcaacagatg ctgtatacgt tacaacagcg
    11641 gatgaatgaa atgtcgctct gaaaactctg gcttcaactg aaccccgtca tcgtacgggg
    11701 ttttttgttt ccggaggtaa gcgttccggg agcggtgcgc ttattcgcgg gggcagcgat
    11761 aagccttatt tactcaacca ttcggatctg tccgataaac agaccatgcg gagttattta
    11821 tggattttga atttacgggt gaagaaaccc cggaacaact ggaaaaaatg ctggaaggac
    11881 ttggggatgt ggatattgac agtcacgcac aggacgtcgt gacggaagat accacggaaa
    11941 aacatgcgga tgaggaagca cagactcaga cgggcgataa caatgtggca ccgacgccgg
    12001 atgccagtgt ggagcagacg caggacgtga aggagccgga agcgaagggg gtgctcaccc
    12061 gcgacggtaa acacgtcatt ccctatgaag tccttgaggc tgaacgttcc ggtaagcaac
    12121 gggccgaaca ggaagccgca cttcttcgtg ggcagatagc tgaagaaaaa cgcagggtgg
    12181 aactactgac gtctcagatc caccaggccg gtatgaagcc cacaccgtta ccggaaaacg
    12241 aaaaaatttc tgatgagcag attgcccgta tcagggagat gtatccggaa attggtgacg
    12301 cggtggcttc gctcatccgt aaaaataact atctccagtc ccgtgttcag caatcaacac
    12361 agcaggcaga aggtaatggt ggtgaggatt tatcaccggt tcttgatgcg atgaatgccg
    12421 tgccggtgct gaaaacgtgg caggagtccg atccggatcg cttctcggtt gctgtatcca
    12481 tcgacgggaa gctccagaat gaccccgcat ggaaagacaa aacgctcact gaacgtttcg
    12541 ctgaagtggc ccgtcgtacg caggttgctt tcggtgaagt cagtgagtcg tctgctgaca
    12601 accaggcaga caaaacggat atccggaaaa cggcggaaga gaaagtgaag accgctgaac
    12661 aggagcaggc agtacctgct tccccgtcag atttaggcac cacggcctcc gtcggaatcg
    12721 gtgataattt tgaacggtta cttggtgctt ctcattcaga ggctgaggcg attatgcgtg
    12781 gtatgacgaa tgctgaaata gacgcacttc tggagaagct cgggtaactt actgaaggag
    12841 tactgaagta atgacgactg taacatcagc ccaggcgaat aagctgtatc aggtggcgct
    12901 ttttactgct gccaaccgca accgctcgat ggttaatatc cttactgaac agcaggaagc
    12961 gccaaaagcg gtttcgccgg acaagaaaag cacgaagcag accagcgcag gtgcgccggt
    13021 tgtccgtatc acagacctta acaaacaggc cggtgatgaa gtgaccttca gcatcatgca
    13081 caaactctca aaacgtccga cgatgggaga tgagcgtgtt gaaggtcgtg gtgaggatct
    13141 cagccatgct gacttctccc tgaaaatcaa tcagggacgt cacctggtgg atgcaggcgg
    13201 acgtatgagt cagcagcgca cgaagtttaa cctggcatcc tctgccagaa cgcttctggg
    13261 gacgtacttt aatgacctgc aggaccagtg tgcgatagtg catcttgctg gagctcgtgg
    13321 tgattttgtt gctgacgaca ctattctgcc gacagcggag caccctgaat tcaaaaaaat
    13381 catgatcaac gatgtactgc ctccgacaca tgaccgtcac ttttttggcg gtgatgcgac
    13441 aagctttgag cagattgaag cggcagatat tttttctatt ggcctggtgg acaatctctc
    13501 cctgttcatt gacgaaatgg cgcatccgtt acagccggtt cgtctgtccg gtgatgaact
    13561 tcacggagaa gatccatatt acgtcctgta cgtcacgccg cgtcagtgga atgactggta
    13621 cacctcgacg tccggtaagg actggaacca gatgatggtt cgggccgtga accgtgcaaa
    13681 aggttttaat catccgctgt tcaaaggtga atgtgcgatg tggcgcaata tcttggttcg
    13741 taagtatgcg ggtatgccga tccgtttcta tcaggggtca aaggttctgg tatcagagaa
    13801 taacctgacg gcaaccacga aagaggtcgc tgctgcaacc aatattgacc gcgccatgtt
    13861 actgggggct caggcgctgg caaatgctta cggtcagaag gcgggcggtc acttcaacat
    13921 ggttgagaag aaaacggata tggataaccg tactgagata gcaatcagct ggatcaacgg
    13981 tctgaaaaaa atccgtttcc ccgagaagag cggcaagatg caggatcacg gcgtgattgc
    14041 cgttgataca gcagtgaagc tctgattttt tcctttcccc atgccgggtt ttcgcccggc
    14101 tttttcagga gtcattaatt atggcaaaga ctatccttgc cccgtcactg agtgaacggg
    14161 tctatacggg tacgcacggt aatgagtcgg tggcagaagg cgtatttacg gtgaatgctg
    14221 cggaagcgga cagtgttatt catcttctct cactgccagt gggcatccgt atcaactcac
    14281 tccagctggt ttcaacgggt ggtctgggta ctgcaaccgt cagcattaag tccggtgagc
    14341 atgctctcat cgataacagc gaagctgttt ctgcaaaatt tgccagatat gtgccagtgg
    14401 agccgtacac cacacagcgt gacggggagc tggttactgt caccattaag actgccgctg
    14461 caaccggcac cctgaatgtt ctgctgcgtt ataccgtggt gggatactga ttaaaacctt
    14521 ccggcccgca tcatgcgggc tttttatccg gggaattata tgagtgagaa aattgccgtt
    14581 gtctatatcg gcccaaaacc cgtgaaaaag gacaccatta ccggaagccg cacattgttc
    14641 ccacgtcttg agccggtgca tgttgacagt gcgatggcct ggcaactgct ggggtttccg
    14701 gatgtctggg ttcgtcatga agagcttgat gatgttctga aaaagcaaca acagaatgag
    14761 cagttgcggc aggcacagca ggcgcaggaa agagtgcttg ctgcgcgggc agaagcggag
    14821 aacagttttg ttgtttctgt taacgggcag gaggtggatt taagtaagct cacctcagca
    14881 cggctggcga cgctgtgtga ggcagaagag ctggatattc acaaagatcc gaaagaaacg
    14941 gctgaggcat tccgtatccg ggtgcgtgag gcatttcgcc gtcgtgttgc ggagactgaa
    15001 cagcatggcg gaactgagtg attttttacc gtatgtccgt cgtcagatca gcggtccact
    15061 gaacattatg atgacggatg ctctgtcaat ggctgccgtg gcattcagcc gtcagtcgct
    15121 ggtgtgccgt cgggaggtta ctgttgtacc agtagcagga aaagaaatcg tgcttccgta
    15181 tgacaaagat gatgaggagt gcgttcatat catccgtatc tctgacgata atcatgagct
    15241 ttttgtcggt cgggatgtgg atatcagctc cggacgctcc ttgcgatttg cctgttctcc
    15301 cggtgaggtg agcgtgcttt atgccgtcgc tccgaaagcc ggacgcagcc agataccgga
    15361 tgaactcctc acatggcctg aagaagtggc tgcgggggca cttgagcggt tgttcatgca
    15421 gactggtgtt tcatggtcag atccgttacg cgcacagtat ttttctgtgc agttttctga
    15481 gggtatccgt cgggcatatc gtcatacact ggcgacaagt ccgtactcct cataccgcaa
    15541 tcctgtccgc agacaaaggt tttactgatg acgacgatta ctgaaatcat cggacgagtg
    15601 aacacgcaac tggttgaccc gatgatggtg cgctggcctc tggctgagct gtgtgattat
    15661 tacaatgatg ctgtgcgggc ggtgattctg gcaaggccgg atgcaggcgc aagcctggaa
    15721 acgctcagtt gtgtccctgg cgcacgccag actttacctg atggcgcaat acagcttctt
    15781 gacgtgatat gcctcagcga tggcagtgcg attaaaccag tatcccggga ggtgcttgat
    15841 gcacagtatc ccgactggca catgctgagg ggaaaaccgg aatgctttat cagcaacgac
    15901 ctgtccccgc gcgtattctg gctgtttcct gtccctgaca aagagataag tattgatgca
    15961 gtggtaagcc ggataccgga ggcagtgtat gttctgacgc aggacgatga tacgccagtt
    16021 ccactggaag aggcttatgt taacccactg gtggactgga tgttgtttcg tgctttcagt
    16081 aaggatgctg ccggtggtgc agaatcgggg ctggctgcgc agcattatca gagttttgtt
    16141 gagcaacttg ggatcaaaca gggggcagac agtgcattgt ctgcccgtaa aaaagtgttt
    16201 aacggaggtg gagtgtgagt gttgttgttt cggggacgct gaaatctcct gatggtgagg
    16261 cgatatcagg agcaaatatt accctgacgg cgctgacagt ttcaccggat gcgctcagcg
    16321 gcaccagtgc gtcggcagtg acccgtgaag gtggatatta cggaatgacg atggatccgg
    16381 gggagtatgc ggtttcggtg acggtgaaag ggaagactgc tgtctacgga cgtgtgcgta
    16441 ttgaggggac cgaaagtacg gtgacgctca atatgctgtt acgccgcagt cttgttgagg
    16501 tgagcatacc cggagagctg ctgacagatt tccggcagat acagaataat gtggctgatg
    16561 accttgctac tattcgtcgc ctgaatgaag acacggcgac aaaaaacact caggccacac
    16621 agtcaaaaga aagtgcagca gccagtgcga agagtgcatc tgacagtgca aagacggcaa
    16681 ccagcagggc ggctgaagcc ggacaaaaag cgactgatgc cactgaggcc gcgacccgtg
    16741 caatcacagc agcgtggaat gcagaggaaa gctcgacccg tgccggagag tctgaaaaag
    16801 ccgccggagc tgatgcagaa aaagccagac agcatgctga aaaggccagg ctggcgcagg
    16861 agagcgccgg agagatcctt aagcgggcag aggctgccac tgtcagtgct gaagaggcca
    16921 gacgtatggc tgagaatgca cgggggcctc agggagaaac tggtccgaag ggggatgtcg
    16981 gtcctaaagg cgaaacaggt ccagtgggcc ctcaagggcc cgcagggccg aaaggtgagc
    17041 gtggtgacgt tggtgctcag ggggctgtag ggcccgctgg tccgcgtggt gagaagggcg
    17101 aacaggggga gcgaggaccg cagggaatac caggcctgaa gggggata
//
LOCUS       DAZFRN010000102        17120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFRN010000102 DAZFRN010000000
VERSION     DAZFRN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.103"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            78..305
                     /locus_tag="ACHKUB_003287"
     CDS             78..305
                     /locus_tag="ACHKUB_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656264.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(382..624)
                     /gene="yfdY"
                     /locus_tag="ACHKUB_003288"
     CDS             complement(382..624)
                     /gene="yfdY"
                     /locus_tag="ACHKUB_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO6656265.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            977..1897
                     /gene="lpxP"
                     /locus_tag="ACHKUB_003289"
     CDS             977..1897
                     /gene="lpxP"
                     /locus_tag="ACHKUB_003289"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO6656266.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2253..2324
                     /gene="ypdK"
                     /locus_tag="ACHKUB_003290"
     CDS             2253..2324
                     /gene="ypdK"
                     /locus_tag="ACHKUB_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO6656267.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2389..3627)
                     /gene="alaC"
                     /locus_tag="ACHKUB_003291"
     CDS             complement(2389..3627)
                     /gene="alaC"
                     /locus_tag="ACHKUB_003291"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO6656268.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4004..5701
                     /gene="ypdA"
                     /locus_tag="ACHKUB_003292"
     CDS             4004..5701
                     /gene="ypdA"
                     /locus_tag="ACHKUB_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO6656269.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5716..6450
                     /gene="ypdB"
                     /locus_tag="ACHKUB_003293"
     CDS             5716..6450
                     /gene="ypdB"
                     /locus_tag="ACHKUB_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO6656270.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6463..7320
                     /gene="ypdC"
                     /locus_tag="ACHKUB_003294"
     CDS             6463..7320
                     /gene="ypdC"
                     /locus_tag="ACHKUB_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6656271.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7323..9818)
                     /gene="ptsP"
                     /locus_tag="ACHKUB_003295"
     CDS             complement(7323..9818)
                     /gene="ptsP"
                     /locus_tag="ACHKUB_003295"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6656272.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9843..10880)
                     /gene="ypdE"
                     /locus_tag="ACHKUB_003296"
     CDS             complement(9843..10880)
                     /gene="ypdE"
                     /locus_tag="ACHKUB_003296"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6656273.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYSAANHRQIGNGPMLVLSDKSLIAPPKLTARIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10880..11965)
                     /gene="ypdF"
                     /locus_tag="ACHKUB_003297"
     CDS             complement(10880..11965)
                     /gene="ypdF"
                     /locus_tag="ACHKUB_003297"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6656274.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEMEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11980..13227)
                     /gene="fryC"
                     /locus_tag="ACHKUB_003298"
     CDS             complement(11980..13227)
                     /gene="fryC"
                     /locus_tag="ACHKUB_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO6656275.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13249..13575)
                     /gene="fryB"
                     /locus_tag="ACHKUB_003299"
     CDS             complement(13249..13575)
                     /gene="fryB"
                     /locus_tag="ACHKUB_003299"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO6656276.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(13794..14759)
                     /gene="glk"
                     /locus_tag="ACHKUB_003300"
     CDS             complement(13794..14759)
                     /gene="glk"
                     /locus_tag="ACHKUB_003300"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO6656277.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14963..16219
                     /gene="yfeO"
                     /locus_tag="ACHKUB_003301"
     CDS             14963..16219
                     /gene="yfeO"
                     /locus_tag="ACHKUB_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO6656278.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16334..16660
                     /gene="ypeC"
                     /locus_tag="ACHKUB_003302"
     CDS             16334..16660
                     /gene="ypeC"
                     /locus_tag="ACHKUB_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO6656279.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16801..>17120)
                     /locus_tag="ACHKUB_003303"
     CDS             complement(16801..>17120)
                     /locus_tag="ACHKUB_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO6656280.1"
                     /translation="LAGQVVMQGFIRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVM
                     SQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTA
                     LGL"
BASE COUNT         4133 a         4597 c         4294 g         4096 t
ORIGIN      
        1 tcagtgaacc attttcagga taactttcta tgaaagtaaa cttaatactt ttcagcttat
       61 ttttattggt ctctattatg gcatgcaatg tttttgcatt ttccatttct ggtggtgtaa
      121 gtgagaggag ctataaagag actgaaaaaa catcagcgat gacgaccaca cactctacaa
      181 aacttcagcc atcacaggcg attttgttta agatgagaga agatgcgcca ccattaaacc
      241 tcacagaaga aatcacgcct acttatccga caaaggcgaa ttatcttatt catcctgtgc
      301 gatagcgtct gtcacatggc aggtgaacaa tcatgtctga tgctgatgtg aacacctgcc
      361 caaatgagcc atcctgaccc actatcgcct gccatacccc accataaagc gaatcatcaa
      421 gccacaagcc ggggcaagaa acataaatat ccccgtcact agttcattac cgccagttaa
      481 ccacatggtg aagtaataaa tgagtgcagc gaggatcacc atgccgaaga aagaagagac
      541 cgccgatacc acgttcagta cctgaccgat gtagccgctt acgcagacaa tacacagggc
      601 gagaaacatc cataaataaa tcatagctca acctcctgcc gcgctaaaaa acctgtaact
      661 tcagtcactt taatagtaag acaatcctta acctctggca atttttgcta agcatcaatg
      721 aaaacagcat gttaaatgca agactgttgt gtacggaaaa atatttactt tgcacgatta
      781 ataatcagta gctgaaagca gtcagcgagt gataaagtac agataccgca ttcatgctcc
      841 tcgtggttat gtcctgacta gtctttacac tctttacagg aaccattgtc gtacatgatg
      901 gcccaaccaa ttgagtgtac cgctgtcgac atggaatctt ctgcgataca acaattcgta
      961 tctacagaag gtaactatgt ttccacaatg caaattttcc cgcgagtttc tacatcctcg
     1021 ctactggctc acatggtttg ggcttggtgt actctggctt tgggtacagc ttccttatcc
     1081 tgttctctgc tttctcggca cgcgtattgg cgcaatggcg cgaccattcc tgaaacgtcg
     1141 tgaatctatc gcccgtaaaa acctggaact ttgtttcccg cagcattctg cggaagaacg
     1201 cgagaagatg attgccgaaa actttcgttc actcggcatg gcgctggtag aaaccggcat
     1261 ggcatggttc tggcccgaca gtcgcgtacg taaatggttt gatgttgaag ggttggataa
     1321 ccttaaacgc gcacaaatgc aaaatcgcgg cgtaatggtt gtcggcgtcc attttatgtc
     1381 gctggaactg ggcggccgcg tgatgggact gtgccaacca atgatggcta cctatcgtcc
     1441 acataataat cagctgatgg aatgggtgca gacccgtggg cgcatgcgct ctaacaaagc
     1501 gatgatcggc agaaataatc tgcgcggcat tgtcggtgca ctgaagaaag gtgaagcggt
     1561 atggtttgct cccgatcagg attatggtcg taaaggcagc tccttcgcgc cgttctttgc
     1621 ggtggaaaat gtcgccacaa ccaatggcac ctatgttctc tcccgtctct ctggcgcagc
     1681 catgttgacc gtaacgatgg taagaaaagc ggattacagc ggatatcgtt tgttcatcac
     1741 cccagagatg gaaggctacc cgacagatga aaatcaagcc gctgcctata tgaacaagat
     1801 tatcgagaaa gagatcatgc gcgcaccgga gcagtacctc tggatccacc gtcgctttaa
     1861 aacgcgcccg gtgggagaat cgtcgttgta catttaacaa actgctctta ctctttccct
     1921 cctcaaatcg gccataataa cctcatggtt tttatggctt ttttcttaag gttttttcct
     1981 tcgctgaata tctaattatt accgtcagaa attaaactgt cgccgatatg agacacttgt
     2041 aagttactaa aagtaaataa gaaaatgtct cttgtctcgt ttcagtttta ggcgtacatt
     2101 agcgccgtct cgagcagttt agctcagaat tctggggtga tgcccacggt aaaatacagg
     2161 gcttttaagg tcgctttgcc cggtgtcaac tcactgtatc aggtgtaatg aagtcattca
     2221 ggcgtaacag taattacgcg gagagatgta aagtgaaata tttctttatg ggcatttctt
     2281 ttatggtcat cgtttgggcc ggtacttttg ccctgatgat ctaaaagcaa actgtgcaat
     2341 gaaaaacagg agccatcagg ctcctgtttg catttctgac gacggtgttt attccgcgtt
     2401 ttcgtgaata tgtttgctgc tggcgggtag taaaccgtcg gcacggaaca tcgctttaat
     2461 cccacgaatc gcctgacgaa tacggtcgcg gttttcaatc agtgcaaagc gaacatgcgt
     2521 atcaccatag tcgccaaagc caatccccgg cgagacacaa accttcgctt cgttaaggag
     2581 cttcttggca aattccagcg atcccatggc cgcatatggt tccgggattt tcgcccagac
     2641 atacatcgaa gccttcggca tttcaaccat ccagcccgct tcatgcagcc ctttaaccaa
     2701 tacatcacgg cggcgtttgt actgttcggc aatgtcgcgc acgcactgtt gatcgccctc
     2761 cagcgccgca atcgccgcca cctgcaacgg cgtaaaggtg ccgtaatcgt gatagctttt
     2821 aatacgagcc agggcgctga ccagcgtttt gttgccaacc ataaagccga tacgccagcc
     2881 cgccatgttg tagcttttcg acagcgtaaa gaactcgact gccacatcgc gtgcacccgg
     2941 tacctgcatg attgacggcg ctttccagcc atcgtagacg atatcggcat aggccaggtc
     3001 gtggaccacc agcacatcgt agcgtttcgc cagcgccaca accttttcga agaactccag
     3061 ctccacgcat tgcgcggttg gattcgacgg gaagccgagg atcatcatct tcggtttcgg
     3121 ataactttca cgaatggcgc gctccagttc gttgaagaaa tcgacacctt ccaccagcgg
     3181 cactgagcgt acctgcgccc cggcaatcac cgcgccataa atatggatcg ggtaacttgg
     3241 attcggcacc agcaccgtgt caccatgatc cagcgtcgcc agcatcagat gcgccaggcc
     3301 ctctttcgaa ccaatagtga cgatggcttc tgattccggg tcgatttcaa cgtcgtagcg
     3361 atcctgatac cagcgggaaa tggcgcgacg taaccgcgga atgccgcgtg aagtggagta
     3421 accatgcgtg tccgggcgct gggccacagt acataatttt tcgacgatat gcggcggagt
     3481 cgcaccgtcc gggttaccca tgctgaaatc gataatatct tcgccgcgcc gacgcgcagc
     3541 cattttcagt tcagcggtga tgttaaaaac gtagggcggg agacgatcaa tgcgcgtaaa
     3601 gcgacgttca gggcgagtgt cagccataat ttcctcagat taacgttagc gcccggaccg
     3661 tccgagcgac gctgccacaa tggtggctgt tttgaaaata gcctgattaa tttctgtctg
     3721 tccagcgttt tagaacattt ttatcaatgg ctttaaatga ggaactccac ttcaaaaaca
     3781 ggaaattttt ctacgctaac aagaaaatta ccaccaattt tattaacatt ataatttcaa
     3841 taaatttacc agccagaagt gcattatcaa gagatacatt gctaatacac catttatctt
     3901 tcaatcccct tttaaattta tggtttttcc tcaaaaacgg cctgctggtc atcagccttc
     3961 aggttaccta tcatagaggt ttaatcctta ttcagagtca cccgtgcacg aaatattcaa
     4021 catgctgctg gcggtcttcg atcgggcagc gttaatgctt atctgcctgt tctttctcat
     4081 ccgtatccgc ctgtttcgcg aactgttgca caagtcggcg cactccccaa aagaattgct
     4141 cgccgttacc gccattttct cgctgttcgc cctgttcagc acctggtccg gcgttcccgt
     4201 agaaggctcg ctggtgaacg tacgtattat cgcggtgatg tccggcggga ttctgtttgg
     4261 cccgtgggta ggcatcatta ccggcgtgat tgcgggtatt caccggtatt taattgatat
     4321 cggcggcgtg acggcgatcc cctgctttat caccagcatt ctggcgggtt gtatatcagg
     4381 ctggataaac ctgaaaatcc ccaaagcaca gcgctggcgc gtcggtattc tcggcggcat
     4441 gttgtgtgag acgttgacca tgattctggt aattgtctgg gcaccaacta ccgcgttggg
     4501 gatcgatatc gtctctaaaa tcggcattcc aatgatcctc ggtagcgtct gtatcggctt
     4561 tattgtgctt ctggtgcaaa gcgttgaggg cgaaaaagag gccagcgccg cgcggcaggc
     4621 caagctggcg ctggatatcg ccaacaaaac gctaccgctg tttcgccatg tcaatagcga
     4681 gtcattacgc aaggtctgcg aaattatccg cgatgacatt cacgccgatg cggtggcgat
     4741 tactaatacc gatcatgtgc tggcctatgt tggcgtgggt gaacataact atcagaatgg
     4801 cgatgacttc attagcccga ctacccgtca ggcgatgaat tacggaaaaa tcatcattaa
     4861 aaacaatgat gaagcccacc gcacaccaga gattcattcc atgctggtga tcccattgtg
     4921 ggagaaaggg gtcgtgaccg gaacgctgaa aatttactac tgccacgcgc atcagatcac
     4981 ctcgtcatta caggaaatgg cggtcggtct gtcgcaaatc atctccacgc aactggaggt
     5041 ttcacgcgcc gagcagctac gtgaaatggc aaataaggca gagcttcgcg ccctgcaaag
     5101 caaaattaat ccccattttc tgtttaacgc tctgaacgct atttcatcgt caatccgtct
     5161 gaatccggat accgctcgcc agttgatctt taatctgtcg cgttatctgc gctataacat
     5221 tgaattaaaa gacgatgagc aaatcgatat caaaaaagag ctgtatcaaa ttaaagacta
     5281 tattgccatt gagcaggccc gctttggtga caagctgacg gttatctatg atattgatga
     5341 agaggtgaat tgctgcattc ccagcctgct gatccagccg ttggtggaga acgccattgt
     5401 ccacggtatt cagccttgca aaggtaaagg cgttgtcacc atcagcgttg cagagtgcgg
     5461 aaatcgggta cgcattgcgg tgcgagatac cgggcacggc atcgatccaa aggtgattga
     5521 gcgggtcgaa gcgaatgaaa tgccgggcaa taaaattggc ctgctgaatg tccatcatcg
     5581 cgtgaagtta ttgtatggcg aggggctgca tatccgccgc ctggagccgg ggacggaaat
     5641 tgcgttttac attcctaacc aacgcacccc agtcgcctca caggctacgt tattgctttg
     5701 agccggagtg atattgtgaa agtcatcatt gttgaagacg aattcctggc acaacaggaa
     5761 ctgagctggc taattaaaga gcacagccag atggagattg tcggcacctt tgacgacggt
     5821 ctggacgtgt tgaagttttt gcagcataac cgcgtcgacg ccatttttct ggatatcaat
     5881 attccgtcgc tggatggcgt gttgctggcg caaaacatca gccagttcgc ccataaaccg
     5941 tttattgtgt tcatcaccgc gtggaaagaa catgcggtag aagcgtttga actggaggcg
     6001 tttgactaca ttctcaaacc gtatcaggag tcacgtatta ccgggatgct gcaaaaactg
     6061 gaagcggcct ggcaacaaca gcagaccagc agtacgcctg ccgcgacggt aacgcgtgag
     6121 aatgacacca ttaatctggt gaaagatgag cgaataatcg tcacgccaat taacgatatc
     6181 tattacgccg aagcgcacga gaaaatgacc tttgtctata cgcggcgtga atcctacgta
     6241 atgccgatga acattaccga attttgcagc aaactgccgc cgtcgcattt tttccgctgc
     6301 catcgctcat tttgtgtcaa tctgaacaaa atacgcgaaa tcgaaccgtg gtttaataac
     6361 acctacattc tgcgactgaa agatctggat tttgaagtgc cggtcagtcg cagcaaagtg
     6421 aaagaatttc gccagttaat gcatctttaa tcgggaactt tcatgaaagc accaggcttg
     6481 cccgccgatc aacaattttt cgccgatctg ttcagcggcc tggtgcttaa cccgcaacta
     6541 ctggggcgcg tctggtttgc cagccagcct gcctcgttac cggtgggcag tttatgtatt
     6601 gattttcccc gtctggatat cgtgctgcgc ggcgaatacg gcaacctgct ggaagcaaag
     6661 cagcagcgaa tggtggaagg agaaatgctg tttattccgg cacgcgcggc taatttaccg
     6721 atcaacaaca aaccggtgat gctgttaagc ctggtgttcg caccgacctg gcttgggtta
     6781 tcgttttacg atagccgcac cacgtcgttg ttgcatcctg ctcgccagat ccagcttccc
     6841 agcctgcaac gcggtgaagg tgaagcgatg cttaccgccc tcacccatct tagccgttcg
     6901 ccgctggagc agaatatcat tcagccactg gtgttaagtt tgctgcatct ttgccgcaac
     6961 gtggtgaata tgccgccggg taattcgcaa ccgcgcggcg attttctcta tcacagcatt
     7021 tgtaactggg ttcaggataa ttatgcccag ccgctcaccc gcgagagcgt ggcgcagttt
     7081 tttaatatca cgcccaatca tctgtcaaaa ctgtttgctc agcatggaac gatgcgtttt
     7141 atcgagtatg tgcgttgggt gcgaatggcg aaggcgagga tgattttgca gaaatatcat
     7201 ctgtcaattc atgaagtggc acagcgttgc ggttttccgg atagcgacta tttttgtcgc
     7261 gttttccggc gtcagtttgg tctgacgccg ggagagtaca gcgcccgttt tcagggctaa
     7321 cgtcagaagg ttaattctgt ttccagcagc gtcaggatac tttgtgcatc ttgcgcggca
     7381 aacaacgact ggcggaagtt tttattcacc agtttacgcg ccagctgcga gaagactttc
     7441 acatgattca tcccttcgtt agcacccagc gtcagcatga tcaccagttc gacttcgccc
     7501 atttctgact gccagtcaat cggtttcgcc agccgggcaa tgctgatact ggaatgacgg
     7561 atccactgag atttggtgtg cgggatcgct acgccaaaac caacgccggt agtgacaatc
     7621 tcttcccgct gccagacatc ttcttccagc tcaaacggat gttcagtacg cccgttaacg
     7681 ccgaggttgc cgcacaggaa ctggatcgcc tgctctttat tgctaaaatc ctgatcaaca
     7741 aagatattct ccagcgccag cagtgggcga acgtcttctt ccggcgtaaa ggcggtgagt
     7801 aacgcttcaa tttcctgcgc gctgcgacat tcacacgcct gacgtgccag ttcccgacac
     7861 gcctcgctat ccagttgacg cagctggctt ttcaccgccg gaatgcgcgg gctactcata
     7921 ctcagctcat ccaggcccag cccaagcagt agcggcagat aacgactttc accgcccagt
     7981 tcaccgcaaa tgcctaccca tttgccccgc tgatgcgcag tggtaactat ttgctgcaac
     8041 atgcgcagga acgatggcgt aatcgggtta tacagcggcg atacgcgcgg gttattacga
     8101 tcgaccgcat acagatactg ggtcatatcg ttagaaccga tactgaagaa atcgacctca
     8161 tcgcagaagt ggtcgatgat gtaacacacc gacggcactt ccaccatgat acccagagta
     8221 atcgtctctg catgacgcag gccatcgcgc ttaagctcaa cgatcgcttt ttggatctcg
     8281 cccttcaccc ataagatctg atcgaggctg tgaaccatcg ggatcatcaa ctgggcgttg
     8341 ccaaaactgg cagcgcgcaa aatcgcccgt agttgagtgc ggaacaggcc agcaaattcc
     8401 gggtaaatac gtaccgcgcg atagccgagg aacgggttct cttcctgggg aatattcaga
     8461 taaggaatgc ttttatcgcc tccgatgtcc attgtgcgga agataatcgg cttgtcgccc
     8521 gctgccagta gcacctgctg gtaggcttca aattgctcct gctcgtccgg cgcgctgtca
     8581 cgatccatat acagcatttc ggtacggaac agcccgacgc cttccgcgcc gttggcaaac
     8641 gcgcctggtg cttccagagc ggtgccaata ttcgccgcga tatcaatacg cttgttatca
     8701 cgggaatagg ccagctgcgc ggcagcctgc gcctgttgtt tttggcgttt atccgccagc
     8761 gtctgcgcaa cctgataata accgctcacc gcgtcattcg ggttaatcgc cagcacgccg
     8821 cactgggcgt caagcaccgc cggttgcccg gcataacggg caatcgcatc cagcggcagg
     8881 ccgctcagta ccgggatcgc cgaggcgcgg gccagaatca gggtatgcga ggtgcgcccg
     8941 gttttctcca gaatcatgcc cgcaagattt ttcaaatcaa ggctcaaaaa ctggcttggg
     9001 gttaaatctt cagccaccag aatggtcggt ttttccagca ccagattgtt gcgcggcttc
     9061 agttccggcc aggtgatatg cagcaactgt tcgctaatat cgcgaatgtc gctaacacgt
     9121 tcgcgcagat aatcgctggc agaagcagaa agcttggcgc aaacctgctc catattgctg
     9181 atgatcgccg cccccagtcc ctgatgctgt tctgtcatca ggcgacggat attgcctgca
     9241 aattcatcat cctgaatcag cgacaaatgg gcgctgagga tagttttgct ttcgccgtca
     9301 cgctcacgca attgctggtt cagttgctcg gcaagcgttg ccaggctgtg ctccagccgg
     9361 gtggaatctt gcgcactggc ggggattgcc cgataactgt cgaggctgtc gctttgtaac
     9421 agggtcagcg tacccacgcc gacgccgctt gccagcacat tgccgtacag taaatccggg
     9481 ttaaggcggc ttaatgaacg cggcagcgga tgcgccgtca gttctgcctg cgtaggctgg
     9541 acgctgtcgc tgtcgataaa gcgcacctgg atgtactctt ccagcacgcg ccgcgcctgc
     9601 tcttcatcgc tgccgctaat gttcaggctg caactgtcat taaatagggt gccggtgcca
     9661 atcagcgcca gcgaactttt cgcatcggcc tttgcgttct ggcgatggtt aataaaggtg
     9721 acttcacttt gccactggct gcactgttct ttaagttccc acgccggacg agcatgcaga
     9781 ccgttaggca aaggacagag aaattgaatc gttaacataa ccgctccggg ttaatcagga
     9841 gatcatctga aatccgtcag ttgaacaacc gtctcacggg taagacgttg aataagggca
     9901 gataaaagtt gctgcatctg caaaatgtcg cggcaatcgg caatcgatgc cgcacaatgt
     9961 ccgtggcgcg tcgctggccc catcaccacc gtgggcacgc cggtgccggt taaatgcacc
    10021 gctccgccgt ccgtgccgcc gttgctgaac atatccgcct gcaacggcac gccaatttct
    10081 gccgccacgg tttcgatcct ggcggtaagt tttggcggcg caatcagcga cttgtcgctt
    10141 aacaccagca tcggcccgtt accaatctgg cgatggttag ccgcgctata atcaaagttt
    10201 ttcgcccagc aggcggtatc aagcacaatg gcgacatccg gcgacaccgc gcgggtggca
    10261 gtttgcccgc cgcgtaatcc cacctcttcg ctggaacttg ccaccagcca cacctccgca
    10321 ggcagttcgg cgtcgtgcag ctcacgtagt aacgtcacca gcagatagca accgaggcgg
    10381 tcatcaaagg ctttccccat cacccgctgg tgagggagaa cctgaaaagt tgtatcaaag
    10441 gtgacgcgat cgcctggacg aattcccgcc tgcatcactt cgtcatagga gcgcgcacca
    10501 atgtccacgc gcatggcgct gacgtcattc ccctgccggt cgccgtcaag caggcctgga
    10561 attttgcact cttcacgggt ggtgatgcgc accggctgca gctggcgggc agccatgcgc
    10621 acgttgccaa ccggcagcac gtcaatagcg ccttcgcggg agatgctgcg caccataaat
    10681 cccacttcgt ccatatgggc gcagatcatc accttcggac ctgtcgattc attgaggcgg
    10741 atcagcaccg atcccagacc atcaaatcgc acttcttttt gcaggcgatc cgcttcttcc
    10801 agtaggatct gccgcacttc ctgttccgag gaggcgatcg catctgcctc gctcaacgct
    10861 tttaatagcg ataaatccat tatgcctctc ccgtgagcaa cactgttttc ggcatggcgt
    10921 agagcacttc tgcgccttgc ggggtaacca gcacaacatc ttcgatgcgc acgccccctt
    10981 gccctggcaa ataaatcccc ggctccacgg tcagcaacat gcctggctgt agcgtcgtgg
    11041 tgtcccgcgg tgaaaaacgc ggatcttcat ggacttcaat gccgatagcg tgaccagtgt
    11101 tatgaccgaa atagtcgcca aatccagcct cggtaatgac ccggcgcgcg gcgtcgtcaa
    11161 tctgctggca gcgcacgccg gggcgaattg cagagattgc cgcgagctgt gcctgcagga
    11221 caatctgata gacgtcaaac agcgggtgag attcggcgct caccccttcg ccattcacca
    11281 gcaaggtgcg cgtcatatca gagcagtagc cctgatacag cgcaccaaaa tcgagagtga
    11341 caaactcgcc cgctgcaaca atcttgtcgc tggctttgcc gtgcggcagc gccccgcgcc
    11401 agccgctggc gacaatggtg tcaaaagagg ctttttctgc gccctgctgg cgcataaacc
    11461 actccagttc agcggctatc tcgcgctcgc tcatccccgc ctgaataaag cggcgaatat
    11521 gctccgctcc gcgatcggca atcccacagg cgaggcggat tatctccacc tcctctggcg
    11581 ttttgatttg ccgcagcaca tccggcgtgg cgctaaccag tttcgcattg agttcagact
    11641 gccagcggtg cgcggtttcc caactcacct gctggccctc aaaaccgagc gtttgcaact
    11701 gttcatcggc aatgatttga ttgacgatag tggtaagcgt gttcgttgcg tcaagcaaat
    11761 gcagctggta gccttgcgca cgggcttcca tctcaacgta atagcgcgaa tccaccagaa
    11821 tgtgcgcact ttcacggcta atcaccacat aacctgatcc ggtggagatc cccagatgcg
    11881 gctgtttgtt ctgccgtgag gagagaagca ctgcatccag ttgttgcgcc ttaagccagt
    11941 cgcgcagcga agcgagtaat gtcatctgga ttttccttct tacaggctat cgattaacaa
    12001 tttgccttta cggaacatca tcagacgcag gaacaccacc atcagcgcag ttatgactgc
    12061 ccccagcgcg atccccgcca tatagacgcc caggttagtg accagcggcc atgcccagat
    12121 agcggactcc gggaaccatt gcactgcgcc cagccagaca gcggcggttg agccgacaat
    12181 agcgccgacc atatacgacg gaatggcggt gatggggctt tccagcgcaa acggaatcgc
    12241 accctcactg atccccataa acgccaggaa catggcggtc ttaccctgcg gataaagttg
    12301 agcgttgaac aggcgtttgc cggttaaacg acggtcgata atggtcgcca gccccagacc
    12361 aatcggcgga ataacgatag cgatagaacg cgcggtaacc ggtaatacgt ggtcggtggt
    12421 aaagctgaag gcaacgaaac ctgccgcttt gttgattggg ccaccaaggt cgattgcggt
    12481 cgccgcggca atccccattg catacatcag cgcgcccttc tcacctgcgg cagtcagcac
    12541 ggtacggata ccgccgttga tccagccacc aaatggggtg atgacatagt acatcgccag
    12601 catgacaaaa atggcggaaa gaatcggtag caaaaatgtg gttttgaacg ctaacaggaa
    12661 atccggtaac tggattttct ggttcatcca cttcacaagg taacccgcga cgatagaaat
    12721 aatcagcgca ccaatgaagg ttgacggcac cggcgaagag gtcgcccatt gcatagtgct
    12781 gggatcgaag ttcagcagtt gcgtcggctg ggtagacatc aagccgccga taaaaccagc
    12841 cgggaacgcc agtttgccgc cgatagagtt cgccacaaaa gcggcaaaca tcggaatagc
    12901 aaagccaaac aacacgccgc cgaaggactg cgacagccag gcaaatttca gtaaggagag
    12961 gtcgaaaccg gagaatttcc cgctattaag agcatccatg atgccgatct cagcaggaat
    13021 tttaagccag ctataagcaa tcagctggct gaaagcgagg atcacaccgc ccataattaa
    13081 ggtcggcacc atgcgtgaaa tccctgacat cacatgctgc gggagttcac cccagaagct
    13141 gcttttagag gcctgcggat tcttaactgc cgctgccgca ccggatgcgc caggcacaac
    13201 ggttgcactg cgttttttaa tggccataac aattatccct gtcgaatatt actgttgttc
    13261 agaggcaatc atctcttcga tttctttgat gatgcctaca gcgtttttaa ttgcgtcctg
    13321 caaagtgatt tcataaacgt cgcgtgattc gaaacgttcg ttatcttccg gggtaactgc
    13381 cacggaatgg atgatgatgg tcgcttcggc gatatcctgc gccgtcaggc gattctggat
    13441 gccgtccgcg ccctgggttt caattttcac ttcataaccg gcttctaccg ccgcttcttc
    13501 cagcgcctga gcggccataa aagtgtgagc caggcccatc gggcaggcac ataaggcaat
    13561 cagtttctta ctcatcgtta attcctcgtt cagtgatagt tgacgagtga aattatgaac
    13621 gtgcaaaaca aatcgccgtt accagacaaa taatgcattt actggagaat taatcccgct
    13681 gtatggaagt gtgacgcagg tcggccttgt gaggggacac aaagaaaaat tgaattaact
    13741 gtatgattta aaagattatc gggagagtta cctcccgata taacaggaag gatttacaga
    13801 atgtgaccta aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg
    13861 acgatgagat acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat
    13921 gcggcacgga aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgccagca
    13981 atataaaccc caccaaaggt tcccagcgtt aatgccagat tgccgccaaa acggcccata
    14041 atgacgcaaa acagcgacaa cgcacggcgg caatcggtgc agctgtcagc cagcgcgcgt
    14101 tcggtaatat cttttggctt gagattttct ggcaggcggt tgtcggcttt aacaattgcg
    14161 cgatacaaat tcaccagccc agggccagaa agcacgcgtt ccgccgaaac atggccaatt
    14221 tccgcacgca atatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg
    14281 tgaccgcctt cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc
    14341 ccaagccctg ttccggcacc gtaaaccgca ataggtttac cttctaccgg ttctgcgcca
    14401 ccaaactgaa tcagatgctc ttttttcagc atcgggatcg ccattgatac agcggtaaaa
    14461 tcgttaataa tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac
    14521 gcccaggtat ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata
    14581 cagccgtctt tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg
    14641 ctggggtaat caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca
    14701 cacagagcaa gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt
    14761 cttcaactgc tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca
    14821 taacaacaac gccggaaagg caggctccct gtaaatatcg atctgggtca cacatttact
    14881 ttatcgtttc agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct
    14941 gaccccgcat aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc
    15001 gctccccgcc gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat
    15061 cgcctcggca ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca
    15121 ggattcacct ctctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt
    15181 tatccgtttc agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc
    15241 accagttccg ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg
    15301 cggcgtcagc ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgcggttgc
    15361 cattggcgct cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc
    15421 cggaaccatc ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac
    15481 cttaaatggc agtagtgaag ttccgctatg ggatcgtctt tttgcgccat taatggcggc
    15541 agcagctggt gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc
    15601 gcattacgga cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc
    15661 catcgcagca gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca
    15721 aatgaaaaat ccggtgctcg tgctgggtat tggcggattt attctcggta ttctgggggt
    15781 tattggtgga ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa
    15841 tcaggctttc agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt
    15901 cgttgctgcc gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt
    15961 ggcattaggg ttgatgctgc atgagcacgt acccgccgta ccagcggcaa taaccgtttc
    16021 ttgcgctatt ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat
    16081 ggcggcggtc gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc
    16141 ggcatggctg ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc
    16201 accccacgat aacgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg
    16261 atacggggct ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg
    16321 aaggagaata acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc
    16381 gcttgcagca aacgcaggtg aaatcaccct actgccatca atcaaattac aaattggcga
    16441 tcgcgatcat tacggtaatt actgggacgg tggtcactgg cgcgaccgtg actactggca
    16501 tcgcaattat gagtggcgta aaaaccgctg gtggcgtcat gataatggct atcatcgtgg
    16561 ctgggataag cgtaaagcgt atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga
    16621 tcatcgcgga aaaggccgcg gacatggaca ccgccattaa acacattcgc caatggaaca
    16681 caatgcctga tgcgacgcta gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt
    16741 agggcggata aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa
    16801 ctacaatccc agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac
    16861 aatcacccag cctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct
    16921 gtcactggtg aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac
    16981 ctgactcata accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg
    17041 caacatggtg actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat
    17101 caccacctgc cccgccagtg
//
LOCUS       DAZFRN010000103        16999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFRN010000103 DAZFRN010000000
VERSION     DAZFRN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.104"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            68..1207
                     /gene="arnB"
                     /locus_tag="ACHKUB_003304"
     CDS             68..1207
                     /gene="arnB"
                     /locus_tag="ACHKUB_003304"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO6656281.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1211..2179
                     /gene="arnC"
                     /locus_tag="ACHKUB_003305"
     CDS             1211..2179
                     /gene="arnC"
                     /locus_tag="ACHKUB_003305"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO6656282.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2179..4161
                     /gene="arnA"
                     /locus_tag="ACHKUB_003306"
     CDS             2179..4161
                     /gene="arnA"
                     /locus_tag="ACHKUB_003306"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO6656283.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYATDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4158..5048
                     /gene="arnD"
                     /locus_tag="ACHKUB_003307"
     CDS             4158..5048
                     /gene="arnD"
                     /locus_tag="ACHKUB_003307"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO6656284.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5048..6700
                     /gene="arnT"
                     /locus_tag="ACHKUB_003308"
     CDS             5048..6700
                     /gene="arnT"
                     /locus_tag="ACHKUB_003308"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO6656285.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6697..7032
                     /gene="arnE"
                     /locus_tag="ACHKUB_003309"
     CDS             6697..7032
                     /gene="arnE"
                     /locus_tag="ACHKUB_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO6656286.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7032..7418
                     /gene="arnF"
                     /locus_tag="ACHKUB_003310"
     CDS             7032..7418
                     /gene="arnF"
                     /locus_tag="ACHKUB_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO6656287.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7412..7678)
                     /gene="pmrD"
                     /locus_tag="ACHKUB_003311"
     CDS             complement(7412..7678)
                     /gene="pmrD"
                     /locus_tag="ACHKUB_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO6656288.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7788..9143)
                     /gene="menE"
                     /locus_tag="ACHKUB_003312"
     CDS             complement(7788..9143)
                     /gene="menE"
                     /locus_tag="ACHKUB_003312"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO6656289.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDESATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9140..10102)
                     /gene="menC"
                     /locus_tag="ACHKUB_003313"
     CDS             complement(9140..10102)
                     /gene="menC"
                     /locus_tag="ACHKUB_003313"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO6656290.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFVRETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10102..10959)
                     /gene="menB"
                     /locus_tag="ACHKUB_003314"
     CDS             complement(10102..10959)
                     /gene="menB"
                     /locus_tag="ACHKUB_003314"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO6656291.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10974..11732)
                     /gene="menH"
                     /locus_tag="ACHKUB_003315"
     CDS             complement(10974..11732)
                     /gene="menH"
                     /locus_tag="ACHKUB_003315"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO6656292.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11729..13399)
                     /gene="menD"
                     /locus_tag="ACHKUB_003316"
     CDS             complement(11729..13399)
                     /gene="menD"
                     /locus_tag="ACHKUB_003316"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO6656293.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     VGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13488..14783)
                     /gene="menF"
                     /locus_tag="ACHKUB_003317"
     CDS             complement(13488..14783)
                     /gene="menF"
                     /locus_tag="ACHKUB_003317"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO6656294.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGETAFLGSSPERLWRRRDNALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14862..15167)
                     /gene="elaB"
                     /locus_tag="ACHKUB_003318"
     CDS             complement(14862..15167)
                     /gene="elaB"
                     /locus_tag="ACHKUB_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO6656295.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15222..15683)
                     /gene="elaA"
                     /locus_tag="ACHKUB_003319"
     CDS             complement(15222..15683)
                     /gene="elaA"
                     /locus_tag="ACHKUB_003319"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6656296.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15748..16665
                     /gene="rbn"
                     /locus_tag="ACHKUB_003320"
     CDS             15748..16665
                     /gene="rbn"
                     /locus_tag="ACHKUB_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO6656297.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTLSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGMLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16853..>16999
                     /locus_tag="ACHKUB_003321"
     CDS             16853..>16999
                     /locus_tag="ACHKUB_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO6656298.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIEL"
BASE COUNT         3944 a         4609 c         4342 g         4104 t
ORIGIN      
        1 aaaatataca caacactctt tatagcaaat ataagtggac aggtattcaa tggcggaagg
       61 aaaagcaatg tcagaatttt tgcctttttc gcgaccagca atgggcgtgg aggaactcgc
      121 tgcagttaaa gaggttctcg aatccggttg gatcacaacc ggtccgaaga atcaggcgct
      181 tgagcaagcc ttttgccagt tgacgggaaa tcagcatgcc atcgcggtca gttcagccac
      241 cgccggaatg catatcacgc taatggcgtt ggaaattggc aagggcgatg aagtgattac
      301 gccttccctg acctgggttt caaccctcaa tatgatttcc ttgttgggtg caacgccggt
      361 aatggtggat gtcgaccgcg atacgctgat ggtcacgcct gaagcgatcg agtcagccat
      421 aacgccacgc actaaagcca tcattccggt gcattatgcc ggtgcgccag cagatattga
      481 cgccattcgc gccattggcg aacgttacgg catcgcagtt atcgaagatg ctgcccatgc
      541 cgtcggtacg tattacaaag ggcgacatat tggcgcaaaa ggtaccgcta ttttttcatt
      601 tcatgccatt aaaaatatta cctgtgcgga aggtggcctg attgtaactg ataatgaaaa
      661 ccttgcccgc cagctacgga tgctgaaatt tcacggtctg ggtgtcgatg cctatgacag
      721 acaaacctgg ggccgtgcac cgcaggctga agtcttaaca ccgggctata agtacaatct
      781 gaccgatatt aacgccgcaa ttgccctgac acagttagca aaattagagc acctcaacac
      841 ccgtcggcgc gaaattgccc agcaatatca gcaagcactg gcagctctcc cctttcagcc
      901 attaagcctt cccgcctggc cgcacgttca cgcctggcat ctgtttatta ttcgtgtcga
      961 tgaacaacgt tgtggtatca gtcgcgatgc gttgatggaa gcgttaaaag aaagaggcat
     1021 tggtaccggg ttacatttcc gcgccgctca cacacaaaaa tattatcgcg agcgttttcc
     1081 cacgctgtcg ttaccgaata ccgaatggaa tagcgaacgc atctgttctt tgccgctgtt
     1141 cccggatatg actaccgccg atgccgaccg tgtcatcaca gcccttcagc aactcgcagg
     1201 acaataagcc atgtttgaaa tccaccctgt taagaaagtc tcggtggtta ttcccgttta
     1261 taacgagcag gaaagcttac cggaattaat caggcgcacc accacagcct gtgaatcgtt
     1321 ggggaaagag tatgagatcc tgctgattga tgacggcagt agcgataatt ccgcgcatat
     1381 gctggtcgaa gcctcacaag cggagaacag ccatattgtg tctattttgc ttaaccgcaa
     1441 ttacgggcaa cattcagcga ttatggcggg attcagtcac gttaccggcg acttaattat
     1501 tacccttgat gccgatctcc agaatccgcc agaagaaatc ccccgcctgg tggcaaaagc
     1561 cgatgaaggt tacgacgtgg tagggactgt acgccagaac cgccaggaca gctggtttcg
     1621 taaaacagct tcgaagatga ttaaccggct tattcagcgc accaccggca aagcgatggg
     1681 tgactacggt tgtatgctgc gcgcctatcg ccgtcatatt gtcgatgcga tgttgcactg
     1741 ccatgaacgt agcaccttta tcccgattct ggcgaatatc ttcgcccgcc gtgccattga
     1801 aattccagta catcatgccg agcgtgagtt tggtgaatcc aaatacagtt ttatgcgcct
     1861 gattaatttg atgtacgacc tggtgacctg ccttaccaca acgccgctac gtatgctgag
     1921 tctgctcggc agcattattg cgattggagg ttttagcatt gcggtgctgc tggtgatttt
     1981 acgcctgacc ttcggaccac aatgggcggc agaaggcgtc tttatgctat ttgccgtgct
     2041 gtttactttt attggcgctc agtttatcgg catgggatta ctcggtgaat atatcggcag
     2101 gatctacacc gatgtccgcg cccgcccccg ctattttgtt cagcaagtta tccgtccatc
     2161 cagcaaggaa aatgaataat gaaaaccgtc gtttttgcct accacgatat gggatgcctc
     2221 ggtattgaag ctctgctggc tgccggttac gaaattagcg ccatttttac ccataccgat
     2281 aatcccggtg aaaaagcctt ttatggttcg gtggctcgtc tggcagcgga aagaggcatt
     2341 ccggtttatg cgacggatga cgttaatcat ccgctgtggg tggaacgcat tgcccaactg
     2401 tcgccagatg tgattttctc tttttattat cgccatctta tttgcgacga aattttgcag
     2461 ctcgctcccg caggtgcatt taatctgcat ggttcgctgt taccaaaata tcgtggtcgc
     2521 gcgccgctga actgggtgct ggtcaacggt gaaacggaaa ctggcgttac attgcaccga
     2581 atggtgaaac gtgccgatgc cggggccatt gtggcgcaac tgcgcattgc cattgcgcca
     2641 gacgatatcg ctattacgct gcatcataaa ttgtgccatg ccgcgcgcca gctactggaa
     2701 cagacattac ccgccattaa acacggtaat attctggaaa tcgcccagcg cgaaaacgaa
     2761 gccacctgtt ttggtcgcag aacgccggat gacagtttcc ttgaatggca taaaccggca
     2821 tccgtactgc acaacatggt acgtgccgtt gccgatccgt ggccgggtgc cttcagctat
     2881 gttggcaatc agaaattcac cgtctggtcg tcgcgtgttc atcctcatgc cagcaaagca
     2941 cagccgggga gcgtgatttc tgttgcgcca ctgctgattg cctgtggcga tggcgcgctg
     3001 gaaatcgtca ccggacaggc gggcgacggc attactatgc agggctcgca attagcgcag
     3061 acgctgggcc tggtgcaagg ttcacgcttg aatagccagc ctgcctgcac cgcccgacgc
     3121 cgtacccggg tactcatcct cggggtgaat ggctttattg gcaaccatct gacagaacgc
     3181 ctgctgcgcg aagatcatta tgaagtttac ggtctggata ttggcagcga tgcgataagc
     3241 cgttttctga atcatccgca ttttcacttt gttgaaggcg atatcagtat tcattccgaa
     3301 tggattgagt atcatgtcaa aaaatgtgat gtcgtcttgc cgctggtggc gatagccacg
     3361 ccgattgaat atacccgcaa cccgctgcgc gtatttgaac tcgattttga agagaatctg
     3421 cgcattatcc gctactgcgt gaagtaccgt aagcgaatca tcttcccgtc aacttcagaa
     3481 gtttatggga tgtgtagcga taaatacttc gatgaggacc attctaattt aatcgtcggc
     3541 ccggtgaata aaccacgctg gatttattcg gtatcaaaac aattacttga tcgggtgatc
     3601 tgggcctatg gcgaaaaaga gggtttacag ttcaccctct tccgcccgtt taactggatg
     3661 ggaccgcgac tggataacct taatgcggca cgaatcggca gctcccgcgc tattacgcaa
     3721 ctcattctca atctggtaga aggttcaccg attaagctga ttgatggcgg aaaacaaaaa
     3781 cgctgcttta ctgatattcg cgatggtatc gaggcgttat accgcattat cgaaaatgcg
     3841 ggaaatcgct gcgacggtga aattatcaac attggcaatc ctgagaacga agcgagcatt
     3901 gaggaactgg gcgagatgct gctggcgagc ttcgaaaaac atccgctgcg ccatcatttc
     3961 ccaccgtttg cgggctttcg cgttgtcgaa agtagcagct actacggcaa aggatatcag
     4021 gacgtagagc atcgtaaacc gagcatccgc aatgcccacc gctgcctgga ctgggagccg
     4081 aaaattgata tgcaggaaac catcgacgaa acgctggatt tcttcctgcg caccgttgat
     4141 cttacggata aaccatcatg accaaagtag gcttacgcat tgatgtcgat acctttcgtg
     4201 gcacccgtga aggcgtgccg cgtctgctgg aaatcttgag taagcataat attcaggcca
     4261 gcattttttt cagcgtcggc ccggacaata tgggccgcca tctctggcga ctggtgaagc
     4321 cacagttttt gtggaagatg ctgcgctcaa atgcggcatc gctttatggc tgggatattt
     4381 tactggcagg tacggcctgg ccaggtaaag agattggtca tgccaatgcc gatatcattc
     4441 gtgaagcggc taaacatcac gaagtcggcc tgcacgcctg ggatcaccat gcctggcaag
     4501 cccgtagcgg taactgggat cggcaaacaa tgatcgacga tattgcacgc ggtcttcgca
     4561 ctctggaaga gattatcggt caaccggtaa cctgttctgc cgctgcgggc tggcgtgccg
     4621 accagaaggt gatcgaagca aaagaagcgt tccatttgcg ctacaacagc gattgtcgtg
     4681 gggccatgcc gttccgtcca ttgctcgaat caggaaaccc tggcactgcg caaattccgg
     4741 tgaccttacc cacctgggat gaagtgattg gtcgggatgt gaaagcagaa gattttaacg
     4801 gttggttact caaccgcatc ctgcgagata aaggcacgcc ggtttatacc attcatgcag
     4861 aagttgaagg ctgcgcttat cagcataatt ttgtggatct cctcaaacgc gcagctcagg
     4921 aaggcgtgac attttgccct ttaagcgaac tgttatcaga gacgttgccg ctcggacaag
     4981 ttgttcgcgg aaatattgcc ggacgtgaag gctggctggg ttgccaacaa attgcgggta
     5041 gtcgctgatg aaatcggtac gttaccttat cggcctcttc gcgtttattg cctgctatta
     5101 cctgttaccg atcagcacgc gtctgctctg gcaacccgat gaaacgcgtt atgcggaaat
     5161 cagtcgagaa atgctggcat ccggcgactg gattgtgccc catctgttag ggctacgtta
     5221 tttcgaaaaa cccattgccg gatactggat taacagcatt gggcaatggc tatttggcgc
     5281 gaataacttt ggtgtgcggg caggcgttat ctttgcgacc ctgttaactg ccgcgctggt
     5341 gacctggttt actctgcgct tatggcgcga taaacgtctg gctctactcg ccacagtaat
     5401 ttatctctca ttgtttattg tctatgccat cggcacttat gccgtgctcg atccgtttat
     5461 tgcattctgg ctggtggcgg gaatgtgcag cttctggctg gcaatgcagg cacagacgtg
     5521 gaaaggcaaa agcgcaggat ttttactgct gggaatcacc tgcggcatgg gggtgatgac
     5581 caaaggtttt ctcgcccttg ccgtgccggt attaagcgtg ctgccatggg tagcgacgca
     5641 aaaacgctgg aaagatctct ttatttacgg ctggctggcg gttatcagtt gcgtactgac
     5701 ggttctccct tggggactgg cgatagcgca gcgggagcct gacttctggc actatttttt
     5761 ctgggttgag catattcaac gctttgcact ggatgatgcc caacatagag ctccgttctg
     5821 gtactacgtg ccggtcatca ttgccggtag cctgccgtgg ctgggattac tccccggtgc
     5881 actgtacaca ggctggaaaa accgcaagca ttccgcaacc gtctatttgt tgagctggac
     5941 gataatgccg ctgctgtttt tctccgtcgc taaaggtaaa ttgcccacct atattctttc
     6001 ctgctttgca tctctggcaa tgctgatggc gcattacgct ttgctggcag caaaaaataa
     6061 tcctctggcg ctgcggatta atggctggat taacatcgct tttggcgtca ctggcattat
     6121 tgccacattt gtggtctccc cgtggggacc aatgaacacg ccggtgtggc aaaccttcga
     6181 gagctataaa gtcttttgtg cctggtcgat tttttcgcta tgggcatttt tcggctggta
     6241 caccttaaca aacgtcgaaa agacctggcc ttttgccgcg ctttgcccgc tggggctggc
     6301 gttgctggta ggattttcaa ttcctgacag agttatggaa ggaaaacatc cgcaattttt
     6361 tgtcgagatg acacaagaat cacttcagcc aagccgctat attcttactg atagcgtcgg
     6421 tgttgccgca ggtctggcat ggagcctgca acgcgatgac atcatcatgt atcgccagac
     6481 aggtgagttg aaatacggcc ttaattatcc ggatgcgaaa gggagatttg tcagcggtga
     6541 tgagttcgca aactggctta atcaacatcg tcaggagggg attattactc tcgtgctttc
     6601 ggttgaccgc gatgaagata tcaacagtct cgccattccg cccgcagatg ccatcgatcg
     6661 tcaggagcgt ctggtgctga ttcagtatcg tcccaaatga tctggctaac attagtcttt
     6721 gccagcttgc ttagcgttgc cgggcagttg tgtcagaaac aggcaacctg ctttgtggcg
     6781 ataaacaaac ggcgcaaaca tatcgtgctg tggctgggac tggcgctggc ttgtcttggt
     6841 cttgccatgg tgctctggct gctggtcttg cagaacgtac cggtaggcat tgcctacccg
     6901 atgttaagtc tgaattttgt ctgggtgacg ctggctgcag taaaactgtg gcacgaaccg
     6961 gtatcgccgc gtcactggtg tggggtggcg ttcattattg gtggcattgt gatcctcggg
     7021 agtacggtgt aatgggcctg atatggggat tattcagcgt cattattgcc tcagttgcgc
     7081 aattaagcct gggttttgcg gcaagtcatc tgccgccgat gacgcactta tgggatttta
     7141 tcgcggctct gttagctttt ggcctggatg cccggatact gttactcggg ctgctgggtt
     7201 atctgctttc cgttttttgt tggtataaaa cgctacataa acttgcgcta agcaaagcct
     7261 atgccttgct cagtatgagt tatgtgctgg tatggattgc ttcaatggta cttcccggct
     7321 gggaagggac tttttcgctg aaagcactac tgggagtagc ctgtattatg agcgggttga
     7381 tgctgatttt cctgcccacg acaaaacaac gttactgagt tttccctgcc gctgtacact
     7441 gccgttccca ctcatcgggg ctataactgc ttgccgagag gactttcacc gtatgcagct
     7501 tttcacgatt aatacaataa agcgcattct gcaaaggcga gagtaaatcc cccactttca
     7561 cggcgaagtc actcttcact tcggcgatca tttttatcgc gccgccagca tcgcagagca
     7621 taagtacatg tctgttatct tgtttattgc agcacgattt tttgaccagc cattccattg
     7681 cattatcctg tttgctaaga gttttcacat caatttacag attaacagat aaactcttta
     7741 tcaacggcaa tggaatggca gaaatgagaa gtgaaacgga gggttaatta ttgctgacgt
     7801 tgcacccact cttttagcgc ctgacgtgaa attttaatac cgccgttttt cagctccggc
     7861 ggtaaagtca gccagcgcac cggttgttga aaacgagcca gcttatcttt cacccattcg
     7921 ccaagatcaa cggtttgctg gtcatactcc accaccgcta ccggccgatg accaaactcc
     7981 ttgtcggcaa cgggaacgat aaacacctgc aaaaccgcag gatgtgcagc aataacacgc
     8041 tcgacttctt ccggctgaat accctcaccg ccactgaaga acagattgtc taaacgtccg
     8101 acaatggtca gcttgccatt atgcatctca ccgcgatcgc gcgtagcgta ccagccttcg
     8161 tcattaacca gtgaaaccag ttgcccgtta cgccagtaac cttctgccat actggcagcc
     8221 cgcagccaca cttcattatt aacgattttc acttcccgac ccggcagcgg cgaaccaacg
     8281 tctgccaggc cgtcggcttc tttcgcacac accgtggagg caaactcggt cagaccatag
     8341 ccgcaaaagc aacgaatccc ctgctcgcgc gcctgttccg tcaactcgac cgggatagcc
     8401 gcgccgccaa gtaacaccgc tttcagggaa acggaactgc ggttaaccag caaacgccag
     8461 agttgtgttg gcaccagtga agcgtgagta cagcctgcca gcatttgctc caatggctgt
     8521 ttatcacgca ccgtcatccg cgcaccagcg tataaccagc gccacataat tccctgaccg
     8581 gagacgtgaa acagcggtaa agagagcaac cagtcatcgt gatcgccaaa cggaatcaac
     8641 gataacaccc cttcggcact ggcaagatgg gcctgataag tatggacagc ggctttcggc
     8701 aacccggtag aacccgaggt caacgtcatt gagcacagac gcgtcggctg ccacgtagcg
     8761 gcatgtgcgc cttcaacccg ctgaatgtgc agcgacgtta acgccggaaa cgtgttttcc
     8821 ccatccggca ccagcgcaaa ttgcagcgtc agattgggca gcaattcttc aagcaacggt
     8881 tgcggcagct gagggttcac gggcaacacc cgcgccccgc attgcagtaa cgccagccag
     8941 gcgagcagcg tttgcggcgt attccacgcc cgcaacatca cgccgctgcc ctcaacaacg
     9001 ccctgcgccg caaagcccgt agctgactca tcgacgcgag cacaaagctc gcgccagtta
     9061 agttgctcgt cattaagacg taaggcgatg gcttctcccc gcacttgccg ccagtgacgc
     9121 cacggccagt cagagaagat cataacaacc gctccagtgc atcaacttcc acgacaggca
     9181 gcgtgctacc cggccagcga cgtacctgct gcgcctgcat cagatccagc gtgtccagcc
     9241 ctggaatggt gtccggcgtt aaccaggcgg caatccgcgc cagttgcgtt aagcctaagc
     9301 tcgattcaat ggaagaactg atcaccgccg tcagccccag cgcgtgcgcc gcctgtacct
     9361 gctcgcgtac tttctccaga ctgcccgtga gcgtgggttt gataactacc gcgcgcacgc
     9421 cctcttcagc cacaaaggca aaatccggct cgcgcaagct ttcatcccag gcaatggcaa
     9481 tgccggtttc acggacaaac gctcgcgaat catcgcgggt tttgcacggc tcttcgagaa
     9541 acgcgatgcg gtggcgataa tccgggttaa catatttggc aaactgctgg cctttcagcg
     9601 gtgtccaggc gcgatttgcg tcaagacgca aatgcagatc cggaattgcc tccagcaaca
     9661 gattaaccac catgccgtcg cgcacggctt cgtacaatcc cactttgacc ttcgccactt
     9721 tctcgcctgg catatcagca agtttgagga tcagatcgtc aggatcgcca ttacacagtg
     9781 gtgccgcacg gtaattggcc gcctgcggga gcgtatctgc cagctccgcc aatgcacagc
     9841 ttacgccaaa ggcaacggaa ggcatctgcg gtagctcgca atcgcctgcc agccagttat
     9901 ttacccaggc aagcagcacg ctttgcgcct cttcccaggt ttcctggctg aagcccggca
     9961 gtggggagat ctccccccac ccttcgcgct cgccctcacg caggcaaacg tacagcccgt
    10021 cgcgggtttt taaccgcctg tcgcgcagaa ccacccccgc gtccatgggg atctgccagc
    10081 ggtatacctg cgcgctacgc attacggatt ccgtttaaat ttgctgaaat caggctgacg
    10141 tttctggttg aaagcgttgc gaccttcctg accttcttcc gtcatgtaga acagcatggt
    10201 ggcgttgccc gccagctcct gcagccccgc ctgcccgtca cagtcggcgt tcagcgcagc
    10261 tttcaggcag cgcagcgcca tcgggctgtt ttgcagcatt tcgcggcacc aacggacggt
    10321 ttctttttcc agatccgcca gcggtaccac ggtgttgaca aggcccatat ccagcgcctg
    10381 ttttgcgtcg tactgacggc acaggaacca gatttctcgc gcttttttct gcccgacgat
    10441 acgagccatg taggaagcgc cccagccgcc gtcgaaggaa ccgactttcg ggccagtctg
    10501 accgaagatg gcattatctg ctgcgatagt caggtcgcac atcatgtgca gaacgtgacc
    10561 accgccgatg gagtagccag ccaccatcgc gacaaccggt ttcggacagg tacggatctg
    10621 acgctggaag tccagcacgt tcaagtgatg tacgccggaa tcatctttat agccgccgta
    10681 atcaccacgc actttctggt caccaccgga gcagaacgct ttatcgcctg cgccagtcag
    10741 aatgatcacg ccgatattgt cgtcataacg cgcatctgcc agtgcctgga tcatctcttt
    10801 taccgtcaga ggacggaagg cattgcgcac ctgcggacga ttaatggtga tttttgcgat
    10861 accgtcggtg gatttttcat aacgaatgtc ctcgaaacct tcggagcagt cgtgccattc
    10921 aaccggtgcg taaagcattg cttcatcagg ataaatcata ttgtgtcctt tagtcagaaa
    10981 cgcaagatct gcgccagact tgcgattacg ccagcgggat tttcccgatg cgcgttatgt
    11041 ccggcgcgag gaatgacatg gcagtcggca gccagttccg ccgccagggc gcggaatttg
    11101 ctgtcacgtt caccacataa ataataaaac gcaaatgtgc gggcactaag gttagcacgt
    11161 aaatcaggct ggacggcgag agaagtcgcc tccagcatgg cggcaagggt tgcgccatta
    11221 ttgttgctgc gcagcgccac cagctcccgg cgttgatcgt cattgagcga ggcaaaaaca
    11281 ggctgttgat accagtcggc aaataccgcc gttaacggtt ctgtgcaaaa acgctgcgcc
    11341 cattggcgat cggaacgctg acgttccgca cgttgttcag cattttgcag ccccggatgc
    11401 ccgccttcga caataacccc acaaagcccc gccagcccct ggcaagccgc catcatcgcc
    11461 acccgtccgc caagcgagta ccccaccagc cagaagtcaa ggatgttgta actaaccaag
    11521 gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc cgccgaacca
    11581 ccatgacctg ggagatcaac atacaaccgt gagtagtcgg caaacgcctc gcccacttct
    11641 tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt
    11701 tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc agaagttgct
    11761 ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg
    11821 tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga cgatgatatt
    11881 tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac
    11941 gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca
    12001 ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt
    12061 aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc
    12121 ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg tacaccgggt
    12181 aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg ttaccaacaa
    12241 acaattgccc ctgttcaggc agatagtcgc agatgcgatg cgccagttgc gcttcgccaa
    12301 acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc gggatttcaa
    12361 cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca atattggcaa
    12421 ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc
    12481 agtactcttc tggttcacag cttgcctgcc attgcaggag ccgtttcccc gtcaggctgc
    12541 ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg gcattgccta
    12601 accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg ccaatcagcg
    12661 gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc
    12721 cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt
    12781 ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca cccagacgct
    12841 gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca aacgggcagt
    12901 tgatatggac tccccccaca tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc
    12961 aacgtgcggg gatatcctgg gtcgggcgcg gcagtgaaat actgtgcgtg gggtgagagg
    13021 cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc tccggcgggc
    13081 gatcggcggt taacaggatc agtttttctc cggttaaccc agcttcaatc agtgccggat
    13141 agagatttgc taccgccgtg ccggaggtca caatcaccgc caccggctgc ttgctgactt
    13201 tcgccagccc cagcgccaga tgtcccagtc cacgctcatc gaaatgggtg tggtgaatga
    13261 atgtggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcca ggggcgatac
    13321 agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt
    13381 taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag tataaggagc
    13441 tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta ttccatctgt
    13501 aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg ctcggggtcg
    13561 gaaccacgga caatgcccgc gccagcatat aatcgcacga cattgccgct aatttttgct
    13621 gagcgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg
    13681 taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt
    13741 aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac agatcacatc atccgctttg
    13801 ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg cagtacctgc
    13861 ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat atcttccacc
    13921 accagcatgt tctcgcgctg gtttttatca tccgccatca gccactcacc taactgctgc
    13981 gcctgcttat catcaggatg atttgctacc gttcccgcca gcgcttcagt acgcagcgcg
    14041 ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcagt ttcgccatca
    14101 aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc catcatcgcc
    14161 gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac cactttgtcg
    14221 agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca gcccgttttg
    14281 tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg cttgatactc
    14341 accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga gctttcgctg
    14401 aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag gcggggtaaa
    14461 agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa gtcggcgtgt
    14521 tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg
    14581 cccaggacgg cagcttcttc atcaccatta cgttgttgcc agtagaattg cgggtacgtt
    14641 tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa aggaatatcg
    14701 ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag tagattttcc
    14761 agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca cattatacgg
    14821 ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg tgccagcaac
    14881 agtcctagta ccagcccaac ggccgcgccc acaccaattc cttgccaggg tttttcgtgg
    14941 acgtagtcat cagcacgata aactgcctgc ttcgcccgat agtaataact gtctgaagcc
    15001 tggctaaccc gttttttcac atcatccagc gctttttctg cacgcgcttt cagctcaaca
    15061 tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt ttcactaagc
    15121 agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct cgttctccat
    15181 ttgcgtaaaa cctgtcacta actatagaca agggttgttg attacgcctg aattacctcg
    15241 cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac agggataaaa
    15301 ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca
    15361 ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca gttgctgccc cactttttcg
    15421 ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag atcatcatca
    15481 cttttcagaa tcctcgcata tgccaccagt tcatcatttt tccaaccaag aatatggcga
    15541 ttatcccccg tcaaatcatc tccatcgata tcctgataag ggcaattctg ctcgacaaca
    15601 aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa ttcagagtga
    15661 tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt atactagccg
    15721 gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac ttcggccggt
    15781 gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca tccgaccctg
    15841 agcggacttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact gcataccgcc
    15901 tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga tcatcttttt
    15961 ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc cttaacgatt
    16021 tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag cggctcatgg
    16081 accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg
    16141 cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta tcgtattgaa
    16201 gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg cgtgccgcct
    16261 ggtccactgt ttcaggaatt aaaagcgggc aaaacaatca ccctggaaga tggaaggcag
    16321 attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc tattttcggc
    16381 gataccggcc cctgcgatgc cgcacttgat ctggctaaag gtgtcgatgt catggtgcac
    16441 gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca tagctctaca
    16501 cgccaggctg cgacactggc ccgtgaggct ggagtcggta tgctaatcat tacccacgtc
    16561 agctcgcgct atgatgacaa aggttgtcag cacctgttac gtgaatgcag gtcaattttc
    16621 ccggcgactg aactggcgaa tgatttcacc gtgtttaacg tttaactctg cttattatta
    16681 aaaacagggc gaaacttgcc ctgttatcgc aacccgcgct acatatatcc acccacacgg
    16741 gcatattgag cgatttttga tatttattca tctcctcacg cactatattc agagcaataa
    16801 gttgcctcta tttatcttga ttcagcacaa cctttgcaac taaaaggagc ccatgatggt
    16861 tacagttgtc agcaattatt gtcaattatc tcaaacgcaa ctcagtcaga catttgcaga
    16921 aaaatttact gtgaccgagg aattactgca gtctttaaaa aaaacagcgt tatccggaga
    16981 tgaagaaagc attgagtta
//
LOCUS       DAZFRN010000104        16853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFRN010000104 DAZFRN010000000
VERSION     DAZFRN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.106"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..230
                     /locus_tag="ACHKUB_003322"
     CDS             <1..230
                     /locus_tag="ACHKUB_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656299.1"
                     /translation="YKDSRLVSCGLNGKTPSPYIHAACEVVRSNIEAQQIHELALALE
                     FARENPECDCPECQHEKEKTTNKTANQATFH"
     gene            338..2410
                     /locus_tag="ACHKUB_003323"
     CDS             338..2410
                     /locus_tag="ACHKUB_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO6656300.1"
                     /translation="MSADKQTFALHCEAKNDKVRKRLDIKGGFFWTEARKLSVAVSRC
                     IAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDTEFCNRYEKGGNDGITMMAIPFD
                     DNINGEDATTAGDDNDNLDGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLF
                     LFSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRAAHHELDKLTNHHLSR
                     LAESVGKAFPHSANHRISPAEFDKFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPS
                     GANAGGGNLTDRGEGFTHNQASLARDIATGVLARSIDVDIYNLHPAHAKRVEEIVSEN
                     KPPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLNKVLTETDHSN
                     PDPEIVEIACGRSSAPMPQRGTAEEIHDDEEKQQTSDAMSNEQAAPESVEEIPVKHNA
                     DTQSLENVSSVETKYQELRAELHEARKNISPKNPVDADKLLAASRGEFVEGISAPTDP
                     KWIKGIQARDTEDQNQSKVEQIAPEAGQNSPDTQQNGPEEQQPAPVAQPELEKNCRVC
                     GQTGGGNCPDCSAVMGDSTYTETFGENDAADGEDSAQTEEKIIQENSVDAAQEGETVV
                     QNEPGSDTSGDDANSEPVTLDWKRQLVIAAVYGLCANPACIATAPAIPDIAIMIANRL
                     ENFGGEKS"
     gene            2407..2703
                     /locus_tag="ACHKUB_003324"
     CDS             2407..2703
                     /locus_tag="ACHKUB_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6656301.1"
                     /translation="MNAWLIPDRIEEQSWARHYQQIAREETEAELADDLEKGLPQHLF
                     ESLCIDNLQRHGASKKAISRAFDDDVDFQERMAEHIRYMAETIARHQINIDSEV"
     gene            2709..3494
                     /gene="bet"
                     /locus_tag="ACHKUB_003325"
     CDS             2709..3494
                     /gene="bet"
                     /locus_tag="ACHKUB_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO6656302.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRRAPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTTERQPERDITPVNEETMSEINALLTSMEKTWDD
                     DLLPLCSQIFRRNIRASSELSQAEAEKALGFLKQKATEHKVAA"
     gene            3491..4171
                     /locus_tag="ACHKUB_003326"
     CDS             3491..4171
                     /locus_tag="ACHKUB_003326"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO6656303.1"
                     /translation="MTPEIILQRTGIDVRGVEQGDYAWQKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQF
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIS
                     FVFGEQWR"
     gene            4417..4668
                     /locus_tag="ACHKUB_003327"
     CDS             4417..4668
                     /locus_tag="ACHKUB_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6656304.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERVDHNELHMIKAEIEKEMHIASLRKMRRTSIN"
     gene            4703..4798
                     /locus_tag="ACHKUB_003328"
                     /pseudo
     CDS             4703..4798
                     /locus_tag="ACHKUB_003328"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4930..6093
                     /gene="intD"
                     /locus_tag="ACHKUB_003329"
     CDS             4930..6093
                     /gene="intD"
                     /locus_tag="ACHKUB_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6656305.1"
                     /translation="MSLFRRGEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVDKLGDMPDVTFEEACLRWLEEKADKKSIDSDKSRIAFWIEHFEGIRIKDISEAM
                     IYSVISKAYNRKTKERWKLQVEAALRKGKEPPAYIPKSVSTQTKATHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVKNKRVRWLEKEEARRLIDACSDPLKSVVKFALATGLRRSN
                     IINLEWQQIDMQRRVAWVNPEDSKSNRAIGVALNDTACKVLRDQIGKHHRWVFVYTTA
                     ARRPDGTMTPSIRKMRLDYNTSWLTACRRAGIENFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDILGDDVPNLSHPEVFEDAKKA"
     gene            6134..6685
                     /locus_tag="ACHKUB_003330"
     CDS             6134..6685
                     /locus_tag="ACHKUB_003330"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO6656306.1"
                     /translation="MSNNFKDLGPEVSFRRIYEYAGGDWQEDNGVWHQNVFAYYLSIS
                     CNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNAEKGHMT
                     KCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQKHGTLAAVAPLPRAHFTKPNIV
                     IKPNANSRPTGDTTGYLANPEEV"
     gene            6687..7541
                     /gene="ynfH"
                     /locus_tag="ACHKUB_003331"
     CDS             6687..7541
                     /gene="ynfH"
                     /locus_tag="ACHKUB_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO6656307.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGVGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLFSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            7584..8198
                     /gene="dmsD"
                     /locus_tag="ACHKUB_003332"
     CDS             7584..8198
                     /gene="dmsD"
                     /locus_tag="ACHKUB_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO6656308.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            8357..9649
                     /gene="clcB"
                     /locus_tag="ACHKUB_003333"
     CDS             8357..9649
                     /gene="clcB"
                     /locus_tag="ACHKUB_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO6656309.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(9602..10297)
                     /gene="bioD"
                     /locus_tag="ACHKUB_003334"
     CDS             complement(9602..10297)
                     /gene="bioD"
                     /locus_tag="ACHKUB_003334"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6656310.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(10422..11642)
                     /gene="mlc"
                     /locus_tag="ACHKUB_003335"
                     /gene_synonym="dgsA"
     CDS             complement(10422..11642)
                     /gene="mlc"
                     /locus_tag="ACHKUB_003335"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO6656311.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(11777..12670)
                     /gene="ynfL"
                     /locus_tag="ACHKUB_003336"
     CDS             complement(11777..12670)
                     /gene="ynfL"
                     /locus_tag="ACHKUB_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6656312.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            12777..14030
                     /gene="ynfM"
                     /locus_tag="ACHKUB_003337"
     CDS             12777..14030
                     /gene="ynfM"
                     /locus_tag="ACHKUB_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6656313.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMATALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(14079..14198)
                     /locus_tag="ACHKUB_003338"
     CDS             complement(14079..14198)
                     /locus_tag="ACHKUB_003338"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO6656314.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            14454..14762
                     /gene="asr"
                     /locus_tag="ACHKUB_003339"
     CDS             14454..14762
                     /gene="asr"
                     /locus_tag="ACHKUB_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO6656315.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            14855..14938
                     /locus_tag="ACHKUB_003340"
     CDS             14855..14938
                     /locus_tag="ACHKUB_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656316.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            15038..15859
                     /gene="ydgD"
                     /locus_tag="ACHKUB_003341"
     CDS             15038..15859
                     /gene="ydgD"
                     /locus_tag="ACHKUB_003341"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO6656317.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPSFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(15898..16227)
                     /gene="mdtI"
                     /locus_tag="ACHKUB_003342"
     CDS             complement(15898..16227)
                     /gene="mdtI"
                     /locus_tag="ACHKUB_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO6656318.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(16214..16579)
                     /gene="mdtJ"
                     /locus_tag="ACHKUB_003343"
     CDS             complement(16214..16579)
                     /gene="mdtJ"
                     /locus_tag="ACHKUB_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO6656319.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
BASE COUNT         4309 a         4270 c         4225 g         4049 t
ORIGIN      
        1 aatataaaga cagtcgtttg gtttcatgtg ggttaaatgg caaaacacca agcccatata
       61 ttcatgccgc atgtgaagtc gtcagaagca acattgaagc acaacaaatc catgaactgg
      121 cgctggctct tgagtttgca agagaaaatc ctgagtgcga ctgccccgaa tgccagcacg
      181 aaaaggaaaa aacaactaac aaaacagcaa accaggcaac cttccactga aataaaaatc
      241 cggcagcgca ggctaccgga tttctccctg cgtcaccgta ttcggagaaa tcagacaaag
      301 ggccgctaat tctaatccag ccagaggttt aaatacaatg agtgctgata aacagacttt
      361 tgcactacac tgcgaagcaa aaaacgataa agtcagaaaa cgccttgaca tcaaaggcgg
      421 ttttttctgg accgaggcca gaaaactttc tgtcgcagtt tcccgctgca ttgcagccat
      481 ggacgatgca ggctacgacg aggatgattt caaaaaaccc gttcgcgtaa atttccccgt
      541 cgtgaatgac cttccaccgg aaggcgtgtt tgatactgaa ttctgcaacc gctatgaaaa
      601 aggcggtaac gatggcatca ccatgatggc tatccccttc gatgacaaca tcaacggtga
      661 agatgccaca actgctggcg atgacaacga taacctggac ggaactattc cggatgatgt
      721 ggagaaaagc gaatccccgg acagcgacga tgactgttct gagtgtgaaa ttcccgtcgc
      781 cactctgagc cttactcatc gcttccttca cctcttctta ttcagcaaag atgaagatgg
      841 aaaataccgg catcatgcca caccagaaca acgcaataac gtgatccgta tggagatgga
      901 cacagaggac agttaccttc agagcctgct tactgctgtg cgcgccgcgc atcatgaact
      961 ggataaactg acgaaccatc accttagtcg cctagctgaa tctgtaggga aagcattccc
     1021 ccactctgca aatcatcgca tcagcccggc tgaattcgac aagttcatct ccacctggat
     1081 gaaaacagac tatgtggatc agggattgct ggcaaaagaa tggcagaaag gaaattatgt
     1141 tacaggcatc actcgtacgc cttccggtgc taacgctggc ggcggaaatc ttaccgatcg
     1201 tggcgaaggc tttacccaca atcaggcatc actggcgcga gacattgcca ctggcgttct
     1261 ggcccgttca atagatgtgg atatttataa cctgcaccca gcacacgcaa aacgcgttga
     1321 agaaatcgtg tcagagaata agccgccctt ttctgttttc cgtgacaaat tcatcgccat
     1381 gcccggtggg ctggattatt cccgcgccat tgtggtggct tcagtgaaag aagcaccaat
     1441 cggcattgag gctatcccgg cgcgcgtgac tgaatatctc aacaaagtgt tgaccgaaac
     1501 cgatcactct aacccggatc cggaaatcgt ggaaatcgcc tgcggtcgct catcagcccc
     1561 aatgccgcag cgcggaacag cagaagagat acatgacgat gaagaaaagc agcaaacatc
     1621 ggacgcaatg tctaatgaac aggcagcgcc tgaatcagtg gaagaaattc cagttaaaca
     1681 taatgcggac acgcaatcac tggaaaatgt ctcatctgta gaaacgaaat accaggaact
     1741 gagagcagaa ctccatgaag ccaggaaaaa catttcgccc aaaaatcctg tcgatgcaga
     1801 caaattgctg gctgcctctc gtggggagtt cgttgaaggc atcagcgccc caaccgaccc
     1861 gaagtggatt aaggggatcc aggctcgcga cactgaggac cagaatcagt ccaaagtgga
     1921 acaaattgcc ccagaagcgg gacaaaacag cccggatacg caacaaaacg ggccagaaga
     1981 gcaacagcca gccccagtag cgcaaccgga gctggaaaaa aactgccgcg tctgtggtca
     2041 gactggcggg ggtaactgcc ctgactgtag tgcggtaatg ggcgatagca cttacacaga
     2101 aacttttgga gaaaatgacg ccgctgatgg agaagactca gcacaaactg aggagaagat
     2161 cattcaggaa aactctgttg atgccgctca ggagggcgaa accgttgttc agaacgagcc
     2221 aggcagtgat acgtccggcg atgacgccaa ttctgagcca gtaactctcg actggaaaag
     2281 acagctcgtg attgccgccg tctatggttt gtgcgccaac cccgcatgta tagccacagc
     2341 gccagcaatc cctgatatcg ccatcatgat tgccaacagg cttgaaaatt tcggaggtga
     2401 gaaatcatga atgcctggct tatccccgat cgcattgaag agcagtcatg ggcacgacac
     2461 taccagcaaa ttgcccgtga agaaactgaa gctgagctgg cagacgacct ggaaaaaggt
     2521 ctgccccaac acctgtttga atcgctatgc atcgataatc tgcaacgtca cggggccagc
     2581 aaaaaagcta tttcccgtgc atttgatgac gatgtcgatt ttcaggaacg catggcagaa
     2641 cacatccgct acatggctga aaccatcgcc cgtcaccaaa ttaatattga ttcagaggta
     2701 taaaacggat gagtacagca ctcgcaacgc tggcagggaa gctggctgaa cgtgtcggca
     2761 tggattctgt cgacccacag gaactaatca ccactcttcg ccagacggca tttaaaggtg
     2821 atgccagcga tgcgcaattt atcgcattgt tgatcgtcgc caaccagtac ggccttaatc
     2881 cctggacgaa agaaatttac gccttccctg acaagcagaa cggcatcgtt ccggtggttg
     2941 gcgttgatgg ctggtcccgc attatcaatg aaaaccagca gtttgatggc atggactttg
     3001 agcaggacaa tgagtcctgt acatgccgga tttaccgcaa agatcgcaat cacccgatct
     3061 gcgttaccga gtggatggat gaatgtcgcc gcgcaccatt caaaacccgc gaaggcagag
     3121 aaatcaccgg accgtggcag tcgcatccca aacggatgtt acggcacaaa gccatgattc
     3181 agtgtgctcg cctggccttc ggatttgctg gcatctatga caaggatgaa gccgagcgta
     3241 ttgtcgaaaa taccgcatat actacagaac gtcagccgga acgcgacatc accccggtta
     3301 acgaagaaac catgtcggaa attaacgccc ttcttacttc catggaaaaa acgtgggatg
     3361 acgacctgtt gccgctctgt tcccagattt ttcgccgcaa cattcgagcg tcgtcagaac
     3421 tgtcccaagc cgaagcagag aaggctcttg gattccttaa acagaaagcc acagagcata
     3481 aggtggcagc atgacaccag aaattatcct acaacgtacc gggatcgacg tgagaggtgt
     3541 cgagcaggga gattatgcat ggcaaaaatt acggctcggg gtcatcacag cttcagaagt
     3601 tcacaacgtg atagcaaaac cccgctccgg aaagaaatgg cctgacatga aaatgtccta
     3661 cttccacact ctgctggctg aggtctgcac gggtgtggcc ccggaagtta acgccaaagc
     3721 cctggcatgg ggaaaacagt acgagaacga cgccagagcc ctgtttgagt ttacttccgg
     3781 cgtgaatatt actgaatccc cgatcatcta tcgcgacgaa agtatgcgta ccgcctgctc
     3841 tccggatgga ttatgcagtg acggcaacgg ccttgagctg aaatgtccgt ttacctcccg
     3901 ggatttcatg aaattccggc tcggtggttt cgaggccata aaatcagctt acatggccca
     3961 ggtgcaattc agcatgtggg tgactcgaaa agatgcctgg tacttcgcca actatgaccc
     4021 gcgcatgaag cgtgaaggcc tgcattatgt cgtggtcgag cgggatgaaa aatacatggc
     4081 tggttttgac gagatggtgc cggaattcat cgaaaaaatg gacgaagcac tggctgaaat
     4141 tagttttgta tttggggagc aatggcgata gccagtaaca atgaggttct catatatgaa
     4201 tatttcattt gaaagtcatg gttcggcagc aagtgttgtt atcaccagtt cgctattcga
     4261 atgccgaaag caccagcata tcgttgatgc actgaagttc aaagtaccgg aaatgaccgt
     4321 cacaacccgg ggatgtttct ggatacggac gacgttagcc acaacatacc tcacggcacg
     4381 ccgtgtttat gatatggcac accaggaata taaccagtgt tcgtcctgat ccagcgcggg
     4441 cagtctttcg tcgatgccaa caactatccg gtggaaatct gcaaggtaac tcttactcag
     4501 gtgatctacc gaagactcga cggcagaacc cgagccactt caattggtgc atttaatgaa
     4561 gaatttgagc gagtcgacca caacgaacta cacatgatta aagcggaaat tgagaaggaa
     4621 atgcatattg ccagccttcg aaaaatgcga cgtacatcaa tcaactgaca accgccttcg
     4681 ggcggttttt aatggcaaaa atatggattc acacagtatc accctcaaag aggcctgtca
     4741 gtttctcaag atatcaaggc caacagctgt taactggata cgaacgggcc gactacaggc
     4801 aacacgaaaa aattcttccg gtaaaagatc accttatctc acaacccggc aagcctgcat
     4861 tgcagcactt cattcaccgc tgcatactgt ccaggtgagc gcgggtgatg gcataacaga
     4921 ggaaagaaaa tgtcactctt ccgcagaggt gaaatatggt acgcctcgta ctcgctcccg
     4981 ggcgggaagc gaattaagga gtctcttggc acaaaggaca agcggcaagc tcaggagttg
     5041 cacgacaagc gaaaagcaga actctggcga gtagacaaac tgggggatat gccagatgtc
     5101 actttcgaag aagcctgcct gagatggctt gaggaaaaag ccgacaagaa atccatcgat
     5161 tccgataaat ccagaatcgc attctggatt gagcatttcg agggtataag gattaaggat
     5221 atatcggagg caatgatcta ctcagttatc agcaaagcgt ataaccgaaa aacaaaggag
     5281 agatggaagt tgcaggtgga ggctgcatta agaaaaggga aagaaccacc agcctatata
     5341 cctaaatcgg tgagcacgca aacaaaagca acacacctgg caatgatcaa ggctattctg
     5401 cgcgccgcag agcgagactg gaaatggctt gaaaaagcac ctgtaatcaa aatacctgcc
     5461 gtaaaaaaca aacgcgtgag atggctggaa aaagaagaag ccaggagact cattgatgca
     5521 tgttctgatc ccctgaaatc tgtagttaaa tttgcactgg caactggcct gaggagatca
     5581 aacattatta atctggagtg gcaacaaatc gatatgcagc gacgtgttgc ctgggtaaac
     5641 cctgaagaca gtaagtcaaa ccgcgctatt ggggtcgcac tgaatgacac tgcctgcaag
     5701 gtgttgcgtg atcaaatagg caaacatcac cgctgggtgt ttgtttatac cactgctgcc
     5761 agaaggcctg acgggacaat gacaccaagc atcagaaaga tgcgcctgga ctataacaca
     5821 tcgtggttaa cagcatgtcg tcgggcagga attgaaaatt tccgttttca tgacctccgc
     5881 catacctggg ccagttggtt aattcagtca ggtgtaccgc tgtcagtact tcaggaaatg
     5941 ggcggctggg agtctatcga aatggtgcgt aggtacgcac accttgcacc taatcatttg
     6001 acagagcacg cgaggaaaat tgacgacata ttgggtgacg atgtcccaaa tttgtcccac
     6061 cctgaggttt ttgaggatgc aaagaaagca taagcaattg aattagaatg agttaagctc
     6121 acgctttcaa gtggtgagca acaactttaa agatcttggc ccggaagtca gtttccgccg
     6181 tatttatgaa tacgctggcg gcgactggca ggaagataat ggcgtctggc accagaacgt
     6241 gttcgcttat tatctctcca tctcctgtaa ccattgcgat gaccccgcat gtacaaaagt
     6301 ctgccccagc ggcgcaatgc ataagcgtga agatggcttt gtggtggttg atgaagatgt
     6361 ttgtatcggc tgccgctact gccacatggc ttgtccgtac ggcgcgccac agtacaatgc
     6421 tgaaaaaggg cacatgacga agtgcgatgg ttgttattcg cgcgtcgccg aggggaaaca
     6481 acccatatgt gtcgaatcct gcccgctgcg tgcacttgag ttcggtccaa ttgaagagct
     6541 gcgccagaag cacggcactc tggctgccgt cgcgccactg ccgcgcgcgc atttcacaaa
     6601 acccaatatc gttatcaaac ctaacgccaa cagccgcccg accggcgata ccactggtta
     6661 tctggctaat ccggaggaag tgtaaaatgg gaaatggatg gcatgagtgg ccactggtta
     6721 tctttaccgt attgggtcaa tgcgtagtag gcgcgctgat tgttagcgga attggctggt
     6781 tcgccgccaa aaatgacgca gaccgtcagc gtattgtccg tggcatgttt ttcctctggc
     6841 tgttgatggg cgtcgggttt attgcctctg tcatgcacct gggatcgcca ctgcgggcgt
     6901 ttaactcgct gaatcgaatc ggagcttctg gtctgagtaa cgagattgct gccggttcca
     6961 tattttttgc cgttggcgga ctatggtggc tggtggcggt catcggtaaa atgccgcaag
     7021 cgttggggaa actctggtta cttttcagca tggcgctcgg cgtcattttc gtctggatga
     7081 tgacctgcgt gtatcaaatc gacactgtgc caacctggca taacggttac actacgctgg
     7141 ctttcttcct gacggtattg ttgagcggtc caatacttgc tgccgcaatt ctgcgggcag
     7201 cccgcgttac atttaatacc acaccatttg ccatcattag cgttctggcg ctgatcgctt
     7261 gtgccggggt gattgtgctg caaggtttgt ctctggcgtc tattcactca tccgtgcaac
     7321 aagccagcgc actggtacca gattacgcct ccttacaggt ctggcgtgtg gtattgttat
     7381 gcgcaggtct tggatgctgg ctttgcccgc tcattcgtcg ccgtgaacct cacgttgccg
     7441 gacttattct ggggctaatc ctcatacttg gcggtgagat gatcgggcgt gtgctctttt
     7501 atggcctgca catgacagta ggaatggcta ttgcaggtta acaaattggt gcggggcaac
     7561 ccgcacattt caggatgtta ggaatgaccc atttttcaca gcaagataat ttttctgtcg
     7621 cggcgcgcgt gttgggcgcg ctgttttatt acgctccaga gagtgcggaa gccgcacctc
     7681 tggttgcggt actcaccagt gacggctggg aaactcagtg gcctctacca gaagcgtcat
     7741 tagcgccgct ggtgacggct tttcagacac agtgtgaaga gactcacgcc caggcctggc
     7801 agcgtttgtt tgtcggcccg tgggcactgc cgtctccgcc gtgggggtcg gtctggctgg
     7861 atcgcgaatc tgtgctgttt ggcgattcaa cattggcact tcgtcagtgg atgcgcgaga
     7921 aaggcattca gtttgaaatg aagcaaaacg aaccggaaga tcattttggt tcactgctgt
     7981 tgatggctgc gtggttggcg gaaaatggtc gccagacaga atgcgaagaa ttgctggcat
     8041 ggcacctttt tccgtggtca acacgttttc ttgatgtttt tatcgaaaaa gcagaacacc
     8101 ctttctaccg tgcactgggt gaactggctc gcttaacgct ggcgcaatgg caatcacaac
     8161 tgttaattcc tgtcgcggtt aaaccgctgt ttcgatagga tcaccgtaat attgccggat
     8221 ggtgacgatt acgcgcaatc cggcaataat aggttacagt gtcacgtttt tttatctctt
     8281 aaagcacgca ctgcttttgc ggctggcctc ttttgccgca aaatagtcgc ccgtgtttca
     8341 ttgcccattt ctgctcatgc atcatctaca catctatccg gatctgcgca ctatgttccg
     8401 ccgtctgctt atcgcaacag tcgtcggtat tctcgcggcc tttgccgttg ccgggtttcg
     8461 tcatgcgatg ctgctactgg agtggttgtt cctcaataat gactccggca gtctggtcaa
     8521 tgcagcgaca aacctttccc cctggcgacg gctgctaact ccggcgctcg gcggactggc
     8581 ggcgggtttg ttgctgatgg gctggcagaa atttacccag caacgccctc atgcgccgac
     8641 cgattatatg gaagcgttgc aaaccgatgg tcagtttgat tacgcagcaa gcctggttaa
     8701 atcgcttgcc tctctgctgg tagtaaccag cggcagtgca attggtcgcg aaggtgcgat
     8761 gattctttta gctgcccttg ccgcctcctg ttttgcccaa cgttttacac cacgccagga
     8821 gtggaaatta tggatcgcct gtggggccgc agcgggaatg gctgcggcct atcgcgcccc
     8881 gcttgctggc agtttattta tagccgaagt gctgtttggc actatgatgt tggcctctct
     8941 cggcccggtg attatttccg ccgtcgtggc attgctggtt agcaatctga ttaatcatag
     9001 cgacgcgtta ctctacagcg tacaactctc agtgacggtt caggctcgtg actatgcgct
     9061 gattatcagt acaggtgtgc tggcaggtct gtgcggacca ctgttgttaa cgttaatgaa
     9121 cgcctgtcat cgtggatttg tgagtctcaa acttgcgccg ccctggcaac tggcactagg
     9181 cgggttgatc gtgggtctgc tttccctgtt cacacctgca gtgtggggca acggctatag
     9241 caccgtacaa tcctttttaa ccgccccacc actgttaatg atcattgccg ggatcttcct
     9301 ttgtaaactg tgtgccgtgc tggcgagtag cggttccggc gcacccggtg gtgtctttac
     9361 accgacgcta tttatcggtc ttgccattgg catgttgtat ggtcgtagcc tgggattatg
     9421 gttccctgat ggcgaagaaa ttacgctttt actcggattg accgggatgg cgacactgtt
     9481 ggcggcaacc acgcacgcgc cgattatgtc gacattgatg atatgtgaaa tgaccgggga
     9541 gtatcagcta ctccccggtt tattgattgc ctgcgtaatt gcgtcggtaa tctcgcggac
     9601 gttacaccgt gactctatct accgccagca cactgcgcag catagctaag cggatgtatt
     9661 gccccagttc acgctgttcg gcgcgcggca gataaggcag ttcaccaatg agcggtgccg
     9721 gaagtttttt accgagcaca tcaatgattt ccgcataatg cgccagtcct gggttgattc
     9781 ggttagccac ccagccaatg agcggcagcc cgtcgttggc aatcgcctga gctgttagca
     9841 gtgcatggtt aatgcaacct tcctgaatac cgacaaccat caacaccggc agttgttcct
     9901 gcaccaccca ttcagagagt ggacgcaaat cattcatcag actgcgccag ccgccagtcc
     9961 cttctaccac gacatgatcg actttttcgg tcaggtttgc caggccgttt gaaatgaggg
    10021 tgtaattgat tgggcaactg tgcgccacgc tactttcttc ttcgcttaac gcgataggat
    10081 taactgcttc ataaggcagt tcgatggttg aaacactctg caacaccagg gcatctttat
    10141 tacgcagccc ttcgggtgtc tctttgctcc ccttcgctac gggtttatat cccgcaaccg
    10201 tttttccctg ggaggctaac gcttgtagca atgcgcggga aaccaccgtt ttccctacag
    10261 aagtgtctgt accggtaata aagaaacgct tcagcatcac taactccacc gttatgcttc
    10321 acaaatataa accaggaaaa taattaacct tgaaagtcta agttatgctt tcctggccca
    10381 aattgagata gcgcaaattt tggtagaaca gttaaaaaat gttaaccctg caacagacga
    10441 atcaacaaag aaccgttata catcgcgtct tttaccagtg cagcgcctgc catcgtgccc
    10501 tggttagaaa actgagtact ctcaacgcta atgtgctgac tatacgcagg aagggcctgc
    10561 tgacggatgc tgtctgagat gaccgggaag aggatatctg ccgctttact taacggtgag
    10621 ccaatcagta ttttttgtgg gttaaataaa ttcaccatga tggcaagaat gcgccctaca
    10681 tgcgcgccca ccccggtaat gatgtctttt gccagcagat cgccgcgcaa tgccgcctga
    10741 cacaatgagt ccacggttaa cggttgtcca tgtaacatcg agctcatgga ttgattaaga
    10801 cgcagctgtg ccagctcaag aatactgtcc acactggcga tggtttcgag gcagccgtga
    10861 ttcccgcaat aacagcgttt cccatacggg tcgacctgtg tgtggcctat ttccacgaga
    10921 ctactgctgc ctgcgtgtag cagatgacca tcggtaatga cgcccgcccc cacgttgtga
    10981 tcgataacca cctgaatcac atcgcgcgcc ccgcgtgagg caccaaacaa ggcctctgcc
    11041 atcgtccatg cgctgatatc atgctgaata taaaccggta cgccggtatg ctgctccagc
    11101 gcctcgccga gcggcatctc ttttacatcc tcatagaacg gcatgcgatg tacaatacca
    11161 ttttccgtat caataattcc cggcaaggtt atggcaatcg aagttagacg ctcaagtttt
    11221 ttctggtggc gaataaaaaa ctgatcgata tgggaaatga tacgatccag caatggcgag
    11281 tcatctttta acgccagttc ctgcgactct tccaccacca gtttgctgct cagatcgcgc
    11341 agagcaagga aaatctcccc gcgactaatg cgcagagaaa gatagtgcca ggcttcagtt
    11401 tcaaccacca gccccaccgc cggacggcca cggttccccg cttctttgat ttccagctct
    11461 tgcaccaggt gtgcttcgag catctcacgg acaattttag tgatactggc aggagccagc
    11521 tgcgccagac gggaaagatc gatacgcgag actggaccaa gctgatcaat caggcgataa
    11581 accgcgcccg cgttggtctg ctttatttga tcaatgtgcc caggctggtt ttcagcaacc
    11641 accgcatact ccctatattt tcgcgctccg aaataatctg taggctatgg tgaagcactt
    11701 caatacgtgt cgtcaaattt ttacttaggc atgtgattaa cagcacattt ttcgggcttt
    11761 ttcgctgaaa tttccctcac ctgagagcat tcagcagatg aatacgaaag ttacgcgcag
    11821 ccggactttg ttcatgatgt ttcggccaga ccaaccacat ttcagaaacc gcatcctctt
    11881 cagcaatcgg cacccagcgc atttcgttga gctgaacacg tttaaatgaa gcaggcaaaa
    11941 ttgaaacacc cagaccggcg gaaaccagac cgatgatggt cattgcctcg cccacctcct
    12001 gagtgatgac gggcgtcaaa tggtaacgtc gcatcagccc gagaatatcg tcatacagcc
    12061 ctgtcccgac gtgcggatca aaaaagacga agggttcttt cgccagttca gccagcgtta
    12121 cattcgggtt atttgccagg ggatgatcgt gcgggatcat cgccataagc ggttcatgga
    12181 cgatgactgc gtgttcaagc gtctccggta acgctgtgtt acgcagcaat cccatatcca
    12241 gcgttccttc aatgagcgga gcgatttgct cacgagtgtt catttcgcgg gtttgtaaat
    12301 ggacatcggg ataatcacgg cgaaacagcg ataacgtatc ggacaccgcc cgaataaaag
    12361 gagcggacga agtaaaacca atgcgcaact cccccgcttc cccctgatgc agtctttcag
    12421 cgcgagcggc agcgtcatct accatagaca ggatttgccg actatctgca agaaactgtt
    12481 ttcctgctgc cgtcagcaat acactgcgat tagttcgtgc cagcagtcgg gcaccaattt
    12541 gttgctccag tgcctgaatc tgctgactta gcggtggttg cgaaatattc aggcgggcag
    12601 cggcgcgccc gaaatgcagc tcttccgcaa cagcaacaaa gtaacgcaga tgacgaagtt
    12661 caatattcat atttaaaaca tcttatttga gattattaat atattagaca gaacaattcg
    12721 attttcctaa cctatgtata agcctgatcc acaggcatat ttagcaagga tttcaagtga
    12781 gccgtactac aactgttgat ggcgctccgg caagcgacac tgacaagcaa agcatttctc
    12841 agccaaatca atttattaaa cgcggtacgc cgcaatttat gcgcgtcacc ctggcgctgt
    12901 tctctgccgg actggcaaca tttgcacttc tctattgtgt gcagcctatc cttccggtgc
    12961 tttcgcagga gtttggctta acgcccgcga acagtagtat ttcactgtcc atttccacgg
    13021 cgatgttggc tattggtttg ctgtttactg gccccctatc cgatgccatt ggtcgcaaac
    13081 cagtgatggc cacggcgcta ctgttggcct ccatttgtac gttactttcg acaatgatga
    13141 ccagctggca cggcattttg attatgcgcg ccttgattgg gctttcgtta agtggcgtgg
    13201 cagctgttgg catgacttat cttagcgagg aaatccaccc cagtttcgtg gccttttcga
    13261 tggggttgta tatcagcggc aactcaattg gcggcatgag cgggcgctta attagtggtg
    13321 tcttcacgga ctttttcaac tggcgaattg ctctggcggc aatcggttgt ttcgcgctag
    13381 cctcggcgtt gatgttctgg aaaatcctcc ctgaatcacg ccattttcgc ccgacttcgc
    13441 tgcgacctaa gacgttgttt atcaactttc gtctgcactg gcgtgaccgg ggattaccgt
    13501 tattgttcgc agaaggcttt ttgctgatgg ggtcgttcgt cacgctgttt aattacatcg
    13561 gatatcggtt gatgctctcc ccctggcatg tcagtcaggc cgtggttggc ttattatcgc
    13621 tggcttattt gaccggtaca tggagctcac ccaaagccgg aaccatgacc acccgctacg
    13681 ggcgcggtcc ggtgatgttg ttttcgacgg gggttatgct gtttggttta ctgatgacct
    13741 tattcagctc gctgtggctg atctttgccg gaatgttact cttctcagca ggattcttcg
    13801 cagcccactc agttgccagc agctggattg gcccccgcgc aaaacgcgct aaaggccagg
    13861 cctcctcgct gtatctgttc agttactatc tggggtcgag tattgccggg acgctgggtg
    13921 gtgttttctg gcataactat ggctggaacg gcgtcggcgc atttattgct ctgatgctgg
    13981 tcattgctct gctggtcggg acgcgtttgc atcgtcgtct gcacgcctga aaaataagcc
    14041 cggactgcgg taactacacg tccggactta ttgcttgctc aaaccaacgt taatagccat
    14101 cctaaaatag acgaagcgcc agccaattcc cgcagcgcgt ctagcgtcat caggattata
    14161 agtacccaaa taaacggatt cattttgctg tgtgtcattt attactgatg cgcagttatt
    14221 ctaccgcttt gtaagtagta aaatagttaa cccgatcaag actactatta ttggtagcta
    14281 aatttccctt aagtcacaat acgttattat caacgctgta atttattcag cgtttgtaca
    14341 tatcgttaca cgctgaaacc aaccactcac ggaagtctgc cattcccagg gatatagtta
    14401 tttcaacggc cccgcagtgg ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa
    14461 aagtattagc tctggttgtt gccgctgcta tgggtctgtc ttctgccgcc tttgctgcag
    14521 agactgcgac cacacctgct ccgactgcga cgaccaccaa agcagcgccg gcgaaaacga
    14581 cacatcataa aaaacagcat aaagcagcac ctgcccagaa agcgcaggcg gctaaaaagc
    14641 atcataaaaa tacgaaagct gaacagaaag cccctgaaca aaaagcgcag gcagcgaaga
    14701 aacacgccgg gaaacacagc catcagcaac cggcaaaacc tgctgcacaa cccgcagcgt
    14761 aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg aaaattacgg tgctaagcgg
    14821 gtaacgttta gcaccgcctt tagcggaggg tagtatggtg ggccgttatc gctttgagtt
    14881 cattcttatc atccttattt tatgcgcact gattgccgcc cgtttttatc tttcctgact
    14941 gtagttatct gattttactc ccactttcat cccgtcccgt ctatagtatt tatgagggtt
    15001 tgcttttaat aatcataatt acccaccaga gtgtgatatg cgtacaacca ttgctgtagt
    15061 gttgggtgca attagtttga cgtctgcttt tgtgtttgca gataaaccag acgttgccaa
    15121 atcggcaaac gatgaggtca gcaccctgtt ttttggtcat gatgatcgtg tgccagtgaa
    15181 tgacacgacc caatcaccgt gggatgcggt tgggcaactg gaaacggcca gcggcaattt
    15241 atgtacggcg acgctgattg cacccaatct ggcattaacg gcaggacact gtttattgac
    15301 gccgcctaag ggtaaagcgg ataaggcggt agcgctgcgt tttgtgtcaa ataaaggtct
    15361 ttggcgctat gacatccacg acatagaagg ccgcgttgat ccgacactgg gaaagcggtt
    15421 aaaagcagat ggggatggtt ggattgttcc tcccgcagct gcaccatggg acttcggctt
    15481 gattgtgctg cgtaatcccc cttctggcat tacaccgttg ccgtcatttg agggagataa
    15541 agccgcgctt actgccgcat taaaatcggc tggtcgtaaa gtgactcagg cgggatatcc
    15601 tgaagatcat ctcgatacgt tatacagtca tcaaaactgt gaagtgactg gctgggcgca
    15661 aacgtcggtg atgtcacatc agtgcgatac cttgccgggt gacagcggtt cgcctctgat
    15721 gttgcatacc gatgacggct ggcaattaat tggggtgcaa agttcggctc ctgccgcgaa
    15781 agatcgctgg cgcgccgata accgggccat ttctgttacc ggttttcgcg acaagctgga
    15841 tcaactgtcg caaaaataat gttcaaacgc tgcccgacag tgcgggcagc gtgttcatca
    15901 ggcaagtttc accatgatca ttccagccaa cagcaagacc aggccaatcc agcctttacg
    15961 atttaaccgt tgaccaaaca agatccaacc tgcggctaac gtggcggcaa taccaaaccc
    16021 gccccacaat gcataagcga cagacaagtc gatccctttt acggcctgag aaagcgcact
    16081 aaaggcagcc agcaccgccg ccagggagag caagccaaat attttgcgac gaaagccgtc
    16141 agaaaatttc aaaaagacgt tagcaacgat ttccagcacg attgccaatg ccagccaggc
    16201 ggcgtgaacc cattcaaact gcgccatggt tcacctccag ttcaggttta cgcgctttac
    16261 gggtacctga tttgatcaac acaatcccgg cgaccagggt ggttaacccg gcaattttca
    16321 tcagcgataa actttcgtcg aataacaaaa cgctaaacaa ggtaataaat aaaataccga
    16381 taccttccca cagcgcataa gctacgccta aggcgatctt tttaacggcg aaagagagaa
    16441 atatatacga cagagaaatc atcaccagca ttaaaataaa gccgccattt ccctcactga
    16501 cgctcgccca tttcattgac agcgtaccgg taatttctgt agcaatagcc aggcctaata
    16561 aaatccaata aatatacatt gtccttctcc tgcaagagaa ttattttaat tttcgcttaa
    16621 ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgcata gggcaagctt aggcagaaga
    16681 aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga gcaagatagt ggatgaggta
    16741 cgaagatggg taaatgttga aaataacgtc ctgaacaaat tgtccataat attacaatta
    16801 tccgcagtgt tgcttctcgt catcgcggat gataattgtc ctcggtagtt gaa
//
LOCUS       DAZFRN010000105        16849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFRN010000105 DAZFRN010000000
VERSION     DAZFRN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.107"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1113
                     /gene="ompN"
                     /locus_tag="ACHKUB_003344"
     CDS             <1..1113
                     /gene="ompN"
                     /locus_tag="ACHKUB_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO6656320.1"
                     /translation="LLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYFSDNSAKD
                     GDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGLKFADYGS
                     FDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFFGLVNGLN
                     FAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAYTSSDRTN
                     DQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVANKTQNFEV
                     TAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATYYFNKNMS
                     TYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1254..1688
                     /gene="uspF"
                     /locus_tag="ACHKUB_003345"
     CDS             1254..1688
                     /gene="uspF"
                     /locus_tag="ACHKUB_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO6656321.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1864..2799
                     /gene="ttcA"
                     /locus_tag="ACHKUB_003346"
     CDS             1864..2799
                     /gene="ttcA"
                     /locus_tag="ACHKUB_003346"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO6656322.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2928..4301)
                     /gene="dbpA"
                     /locus_tag="ACHKUB_003347"
     CDS             complement(2928..4301)
                     /gene="dbpA"
                     /locus_tag="ACHKUB_003347"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO6656323.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4331..4504)
                     /gene="ynaL"
                     /locus_tag="ACHKUB_003348"
     CDS             complement(4331..4504)
                     /gene="ynaL"
                     /locus_tag="ACHKUB_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO6656324.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4779..5762)
                     /gene="zntB"
                     /locus_tag="ACHKUB_003349"
     CDS             complement(4779..5762)
                     /gene="zntB"
                     /locus_tag="ACHKUB_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO6656325.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5776..5823)
                     /gene="ydaN"
                     /locus_tag="ACHKUB_003350"
     CDS             complement(5776..5823)
                     /gene="ydaN"
                     /locus_tag="ACHKUB_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO6656326.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6017..7249
                     /gene="dgcM"
                     /locus_tag="ACHKUB_003351"
     CDS             6017..7249
                     /gene="dgcM"
                     /locus_tag="ACHKUB_003351"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO6656327.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7270..7833)
                     /gene="smrA"
                     /locus_tag="ACHKUB_003352"
     CDS             complement(7270..7833)
                     /gene="smrA"
                     /locus_tag="ACHKUB_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO6656328.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8163..9071)
                     /gene="abgR"
                     /locus_tag="ACHKUB_003353"
     CDS             complement(8163..9071)
                     /gene="abgR"
                     /locus_tag="ACHKUB_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6656329.1"
                     /translation="MTFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9247..10557
                     /gene="abgA"
                     /locus_tag="ACHKUB_003354"
     CDS             9247..10557
                     /gene="abgA"
                     /locus_tag="ACHKUB_003354"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO6656330.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10557..12002
                     /gene="abgB"
                     /locus_tag="ACHKUB_003355"
     CDS             10557..12002
                     /gene="abgB"
                     /locus_tag="ACHKUB_003355"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO6656331.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12039..13565
                     /gene="abgT"
                     /locus_tag="ACHKUB_003356"
     CDS             12039..13565
                     /gene="abgT"
                     /locus_tag="ACHKUB_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO6656332.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13576..14091
                     /gene="ogt"
                     /locus_tag="ACHKUB_003357"
     CDS             13576..14091
                     /gene="ogt"
                     /locus_tag="ACHKUB_003357"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO6656333.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14286..15038
                     /gene="fnr"
                     /locus_tag="ACHKUB_003358"
     CDS             14286..15038
                     /gene="fnr"
                     /locus_tag="ACHKUB_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO6656334.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15190..16140
                     /gene="uspE"
                     /locus_tag="ACHKUB_003359"
     CDS             15190..16140
                     /gene="uspE"
                     /locus_tag="ACHKUB_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO6656335.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKEMIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16190..16447)
                     /gene="ynaJ"
                     /locus_tag="ACHKUB_003360"
     CDS             complement(16190..16447)
                     /gene="ynaJ"
                     /locus_tag="ACHKUB_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO6656336.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16691..>16849
                     /locus_tag="ACHKUB_003361"
     CDS             16691..>16849
                     /locus_tag="ACHKUB_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFO6656337.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTII"
BASE COUNT         4285 a         4204 c         4107 g         4253 t
ORIGIN      
        1 cttttaattc ctgccctgct cgccgcaggt gctgcacatg cagccgaagt ttataataaa
       61 gacggcaaca aattagatct gtatggcaaa gttgatggcc tgcattattt ttctgataat
      121 tcagcgaaag atggcgacca gagctatgcg cgtctgggtt ttaaaggcga aacccaaatt
      181 aacgatcaac tcactggcta cggtcaatgg gaatacaata ttcaggcaaa caacactgaa
      241 tcttcaaaaa accagtcatg gacccgtctg gcatttgccg ggctgaaatt tgcagattac
      301 ggttctttcg attacggacg taattatggc gtaatgtacg acatcgaagg ctggaccgat
      361 atgctgcctg aatttggcgg tgactcttat accaatgcag acaactttat gactggtcga
      421 gccaatggcg tcgcgactta tcgtaatact gatttcttcg gtctggtaaa tggtctgaac
      481 ttcgcggtgc agtatcaagg taacaacgaa ggagccagta atggtcagga aggcaccaac
      541 aacggacgtg atgttcgcca tgaaaacggt gacggctggg gtctttccac aacatatgat
      601 ttaggcatgg gctttagcgc tggtgcggca tacacctctt ctgaccgcac caatgaccag
      661 gttaaccata ctgcggcggg tggtgataaa gcagacgcgt ggactgctgg gctaaaatac
      721 gatgctaaca atatttacct ggcaaccatg tattcagaaa cgcgtaatat gaccccgttt
      781 ggcgacagcg attatgctgt tgcaaacaaa acccagaact ttgaagtcac tgcacagtac
      841 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctg
      901 cacgctgccg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
      961 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacttatgt tgactataaa
     1021 atcaacctgt tggatgaaga tgacagtttc tacgctgcca atggcatctc taccgatgat
     1081 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     1141 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     1201 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     1261 gaacgattct tgtccctatc gatatttccg attcagaatt aactcaacgc gtgattagcc
     1321 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     1381 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     1441 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     1501 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaaatgg
     1561 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     1621 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     1681 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     1741 tgacattttc ccctctaatc cgtaccatac acgccactgt ttttatatca gatttcttat
     1801 gctgggcatt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     1861 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     1921 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     1981 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     2041 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     2101 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     2161 tacaagattg ttgaagagaa tacttacggt atcgtgaaag agaagattcc agagggcaaa
     2221 accacttgct cactgtgttc tcgccttcgt cgcggtatcc tttatcgtac cgcaacggaa
     2281 ctgggggcga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     2341 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     2401 ggcaaacata tcgttattcg tccgctggcc tactgccgcg agaaagatat tcagcgattt
     2461 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     2521 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     2581 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     2641 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     2701 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     2761 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     2821 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     2881 atcccgcata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     2941 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     3001 gcaacagcct gacgcaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     3061 tctgcgccat caagcccgat gtctcctgtc agcgcaccta atacatcacc cgggcgcatt
     3121 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cggcaaaatg
     3181 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     3241 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     3301 cgagctgtac ggccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     3361 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     3421 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgatcgcg ttgctccaga
     3481 tcgccgtgta acgacaatgc actttgccct acttcattca gcgcgtcgca gacagcctgg
     3541 caatcttttt tggtattgca aaacaccaca caagaggatg gctgatgcaa acttaataac
     3601 cgttgcaaca gaggaatttt gcctttactg gatgtctcat aaaattgttg ttcaatgggt
     3661 ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac tcgtccgctg
     3721 attgcggcga tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg agatgcaggc
     3781 gcaaaacgga tgacatcatc aatggcatcg ctaaatccca tatccagcat gcggtcggcc
     3841 tcatccatca ccaaagtatt cagcgcatcc agtgataccg tgcctttttg caagtgatcc
     3901 agcaaacgac ccggcgttgc cacgataata tgcggcgcat gttgcaacga atcacgctgc
     3961 ataccgaacg gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc
     4021 agccgacgca attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa
     4081 gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc
     4141 gttttgccgc tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga
     4201 agcgcggcgg cctgcaccgg cgtcatggtt aaataaccca actcattaag gttcgtgagt
     4261 tgggcgggag gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat
     4321 cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa
     4381 tcggttcttc atcaggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca
     4441 ctggaacagg atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg
     4501 tcatatttcc ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg
     4561 caaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat
     4621 tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc
     4681 tgcaagggag atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg
     4741 gcgtttttgt atacagacag ccggaaaaat tgcttttgtt acaaccattt actacggtgc
     4801 aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg
     4861 aattgccacc cgccgccagg gatcccacca aggttgacgc caaataaccc tgtcagaaag
     4921 gtactgggta aaaagaccat tgccatcaat gacattgtat aggtacgacg agctaaattt
     4981 tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg
     5041 atttcgtcaa gaccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca
     5101 tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc
     5161 atatagcgac gcatcacgat taattgtttg cgcagcagag ccaggaatcc acgcggtgga
     5221 atttgctgat caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata
     5281 aattcgctgg aatgatcggt caacgcatcg cacacatcca ccagccaccc tccgcaatcg
     5341 gtcggacccg tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt
     5401 tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg
     5461 gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc ttcaccgaga
     5521 cggctgactc ggggccgcgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc
     5581 agcggtgttg tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag
     5641 cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca
     5701 tccagcatcc aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc
     5761 acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa
     5821 catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa
     5881 aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc
     5941 agaattatct gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa
     6001 aaataacggg atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc
     6061 tgtctggatt gtttcgccct ttgaggaaca gttaatttat gccaatagcg cggcgcgact
     6121 gttgatgcaa gacctcacgt ttagtcagct acgaaccgga ccctattccg tctcctcaca
     6181 aaaagaactg ccgaaatacc tctccgatct gcaaaaccaa cacgatatta tcgaaatcct
     6241 cactgttcag cgtaaagaag aggaaacagc attaagctgt cggcttgttt tgcgagagct
     6301 gacagaaaca gaaccggtga ttattttcga aggtatcgaa gcgccggcaa cgctgggttt
     6361 aaaagccagt cgctcggcaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt
     6421 tctgactaac tctgcaccaa tgttgttgat tgacccctca cgagatggcc aaatcgtcga
     6481 tgctaacctc gccgcgctca atttctatgg ttataaccat gaaacgatgt gccagaaaca
     6541 tacctgggaa attaatatgc tcgggcgtcg cgtcatgcct atcatgcatg aaatttcgca
     6601 tttacccggc ggccataaac ctttgaattt tgttcataaa ctggcggatg gatctactcg
     6661 tcatgtgcag acctatgccg ggccgattga aatttatggc gacaagctca tgttatgtat
     6721 tgtgcatgat attactgagc aaaaacggct ggaggagcag ctggaacatg ctgctcacca
     6781 tgacgcgatg accggattac tgaatcggcg acagttttat cacattaccg agccaggcca
     6841 aatgcagcat ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt
     6901 taaacacatt aacgatctct atgggcattc taaaggtgat gaggtgttat gcgccctcgc
     6961 ccgcaccctc gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga
     7021 gtttgtctta ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat
     7081 ccgcgtaagc gtggcaaaag tgagtatttc gggcttacca cgctttaccg tcagcattgg
     7141 tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc
     7201 tttgtatcgg gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccgcggatgc
     7261 gtctcgagat caacgactgc gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc
     7321 cgttttacgt agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcagtgca
     7381 atatgcctga acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt
     7441 ggcatgcgat ttatcatccc gtcctttgcc atgaataatc agcacgttac gcaaaccatc
     7501 agccatggct tgttgaatga aactgaacat cattttgcgg cactcttcca ccggctggcg
     7561 caaaagattc aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc
     7621 atgttgcaac ccttcccgcc gaaactccag cggctgactt agtgggatga tgtcgagaaa
     7681 tccggtggtg aggaaattat caagctgcag cgtgtcgata cgctgcggcg cacgttggtt
     7741 acgcgttgga tgccagtgga catcggtagc acgtttcagc ggctggacat cttccatggc
     7801 gtcaagaaac agcgatttgt cgtcaaggtt catgttacat cctcccgcaa ttaagagcgc
     7861 gatatgataa ccagaccggg tcggtccaac aacgtattac ccaaaattcc agtaataagt
     7921 tccaaatatt gccgatattt taagcaaaat gcttatgcat gattattcat tcacgatatt
     7981 aataatgtaa cttatatttt cgtgaaatct gtcactgaag aaaattggca actaaaggtt
     8041 aaaaccgtta taacacagtc accggcgcag aggagacaat gccggactta agacgcggat
     8101 gcactgctgt gtgtactgta gagtctggcg gatgtcgaca gactctattt ttttatgcag
     8161 ttttaacttt gcagatagcc gcattctcgc cggaattgcg tgattaatga cgcggtcagt
     8221 ggtgtctggc gactatcacg ccgctgaatc aaataatagg ccgctttcgg taaaatttcg
     8281 ctaaccggca acatcaccag cccctgtccg tgcaagggat cgcagcccat ttcttcaggc
     8341 agtatgctga gaaaatcgct ttttgccacc agactgatac aggctgagaa cgtctcgcag
     8401 actacaccga cctgtggcgt ttgcgcctga tcgtcaagca attcactcaa ctgtttgtag
     8461 taactgccgt gtggcgtcgg cattgtccag ctgtaatcca gtaactgttt gatcgaacgg
     8521 gcaccaatgg cggggtgtcc cgggcggcaa aagatcgcga attgcttttc cagtaatttc
     8581 tcaaaagtaa attcgtggtc gtacggtccc tgataatagg tattgatggt gaaatccaat
     8641 tctccctgac gcaattcatt aatcatcgac accagttgcc cttccataat gcgtactttt
     8701 acctgcggat gctgctgatg aaaacgagat atgacagctg gcatcagact gcgggaaata
     8761 ctggccccca tgccgatatt aatctgccct gccagttgcc cttgtcgttg gcgaatttcc
     8821 tcttgcgctg cgcgcagctc ttcaagaata agactggcgt gctgataaaa actttcaccg
     8881 gcatcagtta acgtcacgcc tttactacgg cgaaaaaaga gttgcgccgc taatccttct
     8941 tccagctcct gaatagattt actcagtgcc ggttgcgaca tattcaacat tcggctcgct
     9001 ccgcgaatgc tgccctgacg agccacttca acaaaagcac gaatttgatg aatttttacc
     9061 tgaaaagtca tgacgccacc gataaccgtt atttatcaga ccaaagaaac tggcatctac
     9121 tttaatgcag atgattgtgt cagggtaatt tatgaacggt taaaactgtg aaaaatcagt
     9181 tagtgataag taaaaactat cgctacgtga accgggtcac actttttact gatgacggga
     9241 aaggttatgg agtctttgaa tcaatttgtt aattcgcttg ccccaaaatt atcgcactgg
     9301 cgacgtgatt ttcatcacta tgcagagtct ggctgggtgg aattccgcac tgccaccctt
     9361 gttgcggaag aattgcagca gctcggctat tcactggcgc tgggccgcga agtagttaat
     9421 gaaagtagcc ggatgggact acctgatgaa ctcactctac aacgcgaatt cgagcgcgct
     9481 cgtcaacagg gtgcgctagc acaatggatt gcggtttttg aaggtggttt cactggtatc
     9541 gtcgccaccc tggataccgg tcgccccggt ccggtgatgg ctttccgtgt cgatatggac
     9601 gcgctggatc tcagtgaaga gcaggatgtc agccatcgcc cctaccgcga cggttttgcg
     9661 tcatgtaacg ccggaatgat gcatgcctgt ggtcatgatg gacataccgc cattgggctt
     9721 gggctggcgc atacccttaa acaattcgag tccggactac atggcgtcat caaactgatt
     9781 tttcagcctg cagaggaagg tacgcgtggc gcgcgggcga tggtcgatgc aggtgtcgta
     9841 gatgatgttg attattttac tgccgtgcac attggcactg gcgtacctgc gggcaccgtg
     9901 gtgtgcggca gtgataattt tatggcaacc accaaatttg acgcgcactt caccgggacc
     9961 gccgctcacg caggcgcaaa accagaagac ggtcacaatg ccttgttggc ggcagcacaa
    10021 gcaactcttg cactgcatgc aatcgccccg cacagcgaag gagcttccag agtaaacgtg
    10081 ggcgttatgc aggcaggaag cggccgtaac gttgttcctg cctcggcgtt gctgaaagtg
    10141 gaaacacgcg gggccagcga cgtcattaat caatatgttt ttgaccgtgc acaacaagcg
    10201 attcagggcg cagcaaccat gtatggtgtc ggcgttgaaa ctcgtctgat gggtgcagct
    10261 accgccagtt ctccttcgcc gcaatgggtc gcatggttgc aaagtcaggc ggctcaggtc
    10321 gcgggggtca atcaggccat tgaacgtgtt gaagcgcctg cgggttccga agatgccaca
    10381 ttaatgatgg cccgcgtgca gcaacatcaa gggcaagcct cctacgtggt gtttggcaca
    10441 cagctggcgg caggtcatca caacgaaaaa ttcgattttg acgagcaggt tctcgctatt
    10501 gccgtcgaaa cgctggcgcg caccgcgctc aattttccct ggacgcgagg tatctgatgc
    10561 aggaaatcta tcgttttatc gacgatgcga ttgaagccga tcgccaacgt tataccgata
    10621 ttgccgatca aatctgggat catccagaaa cacgttttga agagttctgg tcagcggagc
    10681 atctggcttc ggcgctggaa tctgcaggct tcaccgttac ccgcaacgta ggcaatatcc
    10741 caaatgcctt tattgcttcg tttggtcaag gcaaaccggt tatcgccctg ctgggagaat
    10801 atgacgccct ggcaggttta agtcagcaag caggttgcgc gcaacctaca tccgtgacgc
    10861 ccggtgaaaa tggtcacggt tgcggacaca atttgctggg aaccgccgcc tttgccgctg
    10921 caatagccgt caagaaatgg ctggaacaat atgggcaagg cggcacagtg cgcttttatg
    10981 gttgtcctgg cgaagaaggc ggctcgggta aaacgttcat ggtccgcgag ggggtatttg
    11041 atgatgtgga tgcggcactc acctggcacc cggaagcctt tgccggtatg ttcaataccc
    11101 gcacgctggc aaacattcag gcatcatggc gctttaaagg gatcgcagca catgccgcga
    11161 attcccctca tttgggacga agcgcccttg atgccgtaac gttgatgacc actggcacca
    11221 acttcctcaa cgaacatatt attgaaaaag cgcgcgtaca ctatgccatc acagatagcg
    11281 gcgggatctc gcccaacgtg gtccaggcgc aggcagaagt gctttatctt atccgtgccc
    11341 ccgaaatgac cgacgtgcag catatttatg atcgggtcgc caaaatcgcc gaaggtgcgg
    11401 cattgatgac cgaaaccacg gttgaatgcc gcttcgacaa agcctgttcc agttatctcc
    11461 cgaatcgcac cttagaaaat gccatgtacc atgccctatc ccattttggt accccggaat
    11521 ggaactccga agaactggct tttgcgaaac aaattcaggc tacgctcacc cccaacgatc
    11581 ggcaaaacag tctgaataat atcgccgcaa ccggtggcga aaacggcaag gtttttgcac
    11641 tacgtcatcg tgaaacggta ctggcgaatg aggtcgctcc atatgccgcc accgataacg
    11701 tgcttgcggc atcgactgat gtcggcgacg tcagttggaa actgcctgtt gcccagtgtt
    11761 tcagcccctg ttttgccgtc ggtacaccgc tacatacgtg gcaactggtt agccaggggc
    11821 gaacatctat tgctcataaa ggaatgctgc tggcggcgaa aactatggca gcaaccacag
    11881 tcaatctctt cattgattca gggctattgc aagaatgcca acaagagcat cagcaagtta
    11941 cggacacgca accgtatcac tgccctatcc cgaaaaacgt gacaccgtca cctttaaaat
    12001 aacaacaaca acgcaaacac aacaaccgag gaatgcccat gagtatgtca tccataccgt
    12061 cgtcctccca atccgggaag ctctatggct gggtcgaaag aattggtaac aaggttcccc
    12121 atcctttttt gctctttatc tatttgataa tcgtactcat ggtgacgacg gcaattttgt
    12181 cagcctttgg cgtcagtgcg aaaaacccga ccgatggtac gccggtggtg gtgaaaaacc
    12241 tgctcagtgt ggaaggatta cactggtttt tacccaatgt gattaaaaac tttagcggtt
    12301 ttgctccact tggagcgatc ctggcgctgg ttttaggtgc cggtctggcg gagcgcgtcg
    12361 gcttactgcc agcgctaatg gttaaaatgg catcgcatgt taatgcccgc tacgccagtt
    12421 atatggtgct gtttattgct tttttcagcc acatttcttc cgatgcggcg ttagtgatca
    12481 tgccaccgat gggtgcgctg atttttctgg cggtgggcag gcatccagtt gcaggtttac
    12541 tggctgccat tgcaggcgta ggttgcggct ttacggctaa tttactgatt gtcacaaccg
    12601 acgtgttgct gtcggggatc agcacggaag cggcggctgc gttcaatccg caaatgcacg
    12661 tcagtgtaat tgataactgg tattttatgg ccagctccgt agtcgtactg acgattgttg
    12721 gcggcctgat aaccgacaaa atcatcgagc cacgattagg tcaatggcag ggaaacagcg
    12781 atgagaaact gcagacattg accgaaagtc agcgttttgg tttacgcata gcaggtgtcg
    12841 tatcgctact ttttattgct gcgatcgcgc tgatggtgat cccggaaaac gggatattgc
    12901 gcgatccgat taatcacacc gtgatgccat caccctttat taaaggtatc gtgccactga
    12961 tcattctttt tttctttgtt gtctcgctgg cttatggcat cgctacccgc acaattcgac
    13021 gtcaggcgga tttaccgcat ttaatgattg aaccgatgaa agagatggcg ggatttatcg
    13081 tgatggtttt tcccctcgcc caatttgtcg ccatgtttaa ctggagcaac atggggaaat
    13141 tcatcgccgt ggggctgacc gatatactgg aaagttcagg gcttagcggc ataccggcgt
    13201 ttgtcggtct ggcgttgctt tcctctttct tatgcatgtt tattgccagc ggttccgcaa
    13261 tctggtcgat tctggccccc attttcgtac caatgtttat gctacttggc tttcacccgg
    13321 catttgcgca aatcctcttt cgtattgccg actcatccgt attgccttta gcgccggtat
    13381 ctccttttgt cccactgttt cttggattcc tgcaacgcta caaaccagac gcgaaactgg
    13441 gtacttacta ttcgttagtc ttgccctatc cgcttatctt tttggtggta tggctgctga
    13501 tgttgctggc gtggtatctt gttggtctgc cgataggtcc ggggatttac ccacgtttgt
    13561 cttaagagag aacggatgct gagattactt gaagaaaaaa ttgccacgcc actgggtcca
    13621 ctgtgggtga tttgcgatga gcaatttcgc ctgcgggcgg ttgaatggga agagtacagc
    13681 gaacgcatgg tgcagctgct ggacatccat tatcgcaaag aaggctatga gcgcatttct
    13741 gccaccaacc caggcggttt aagcgacaag cttagtgatt attttgccgg taatcttagc
    13801 attattgata cgcttcccac tgccacaggg gggacgccat ttcagcgcga agtctggaaa
    13861 actctacgca ctatcccctg cgggcaggta atgcattacg gccaactggc tgaacaattg
    13921 ggccgccctg gcgcggcgcg tgccgttggt gcggcaaacg gatcgaatcc catcagcatc
    13981 gtcgtacctt gccatcgggt tattggccga aacggcacca tgaccggata tgcaggcgga
    14041 gttcagcgaa aagagtggtt attgcgccac gaaggttatc ttttgctgta aacattaaac
    14101 aatttgtgcc agcttgttca cacttttatg taaagttacc cttaacaact taagggtttt
    14161 caaatagata gacatatatt tacatctaat atcggaattc tctgctgtta aggtttgctt
    14221 agacttactt gctccctaaa aagatgttaa aattgacaaa tatcaattac ggcttgagca
    14281 gacctatgat cccggaaaag cgaattatac ggcgcattca gtctggcggt tgtgctatcc
    14341 attgccagga ttgcagcatc agccagcttt gcatcccgtt cacactcaac gaacatgagc
    14401 ttgatcagct tgataatatc attgagcgga agaagcctat tcagaaaggc cagacgctgt
    14461 ttaaggctgg tgatgaactt aaatcgcttt atgccatccg ctccggtacg attaaaagtt
    14521 ataccatcac tgagcaaggc gacgagcaaa tcactggttt ccatttagca ggcgacctgg
    14581 tgggatttga cgccatcggc agcggccatc acccgagctt cgcgcaggcg ctggaaacct
    14641 cgatggtatg tgaaatcccg ttcgaaacgc tggacgattt gtccggtaaa atgccgaatc
    14701 tgcgtcagca gatgatgcgt ctgatgagcg gtgaaatcaa aggcgatcag gacatgatct
    14761 tgctgttgtc gaagaaaaat gccgaggaac gtctggctgc attcatctac aacctgtccc
    14821 gtcgttttgc ccaacgcggc ttctcccctc gtgaattccg cctgacgatg actcgtggcg
    14881 atatcggtaa ctatctgggc ctgacggtag aaaccatcag ccgtctgctg ggtcgcttcc
    14941 agaaaagcgg tatgctggca gtcaaaggta aatacatcac catcgaaaat aacgatgcgc
    15001 tggcccagct tgctggtcat acgcgtaacg ttgcctgatt tttccgcata actcactatc
    15061 cttctgtcat atcattaaat ttttctgatt tattgatctg gcagaaggtt catcactgtt
    15121 tcattcacca gatatgggtt aatcttttaa ttacaaactg cgttgacagt tgttgtaagg
    15181 agaccctgta tggctatgta tcagaacatg ctcgttgtta tcgatcctaa ccaggacgac
    15241 caaccagcat tgcggcgagc tgtttattta catcaacgga ttggtggcaa aattaaagcc
    15301 tttttgccga tctatgactt ctcatacgaa atgaccaccc tgctctcccc ggacgaacgt
    15361 accgctatgc gtcagggcgt catcagccag cgtacagcct ggatccacga gcaggcaaaa
    15421 tattatctca atgctggcgt tcccattgaa attaaagtgg tctggcataa ccgtcccttc
    15481 gaagccatca ttcaggaagt gatcagcggc gggcacgatt tggtgctaaa aatggcgcac
    15541 caacatgacc gtctggaagc ggtgattttt acgccaacgg actggcatct gttacgcaaa
    15601 tgcccaagcc cggtgtggat ggtgaaagac cagccgtggc cggaaggagg caaggcgctg
    15661 gtggcggtga atctcgccag tgaagagccg tatcataatg cgctcaatga aaaactggtc
    15721 aaagagatga tcgaactggc agaacaagtc aaccataccg aagttcatct ggttggcgct
    15781 tatccggtaa cgccaattaa tatcgcgatt gaactgccgg aatttgatcc gagcgtttat
    15841 aacgatgcca ttcgtgggca acatttgctg gcaatgaaag ctctgcggca gaaattcggc
    15901 attaatgaaa acatgacgca cgtagaaaaa ggtctgccag aagaggtgat tcctgatttg
    15961 gcggagcact tacaggcggg gattgtggtt ctgggcacgg taggacgcac cggtatttca
    16021 gcagcattcc tcggcaacac ggcggaacag gtgattgatc atcttcgctg cgatctgttg
    16081 gtcattaaac ctgaccagta tcagacaccc gttgaactgg atgacgaaga agacgattaa
    16141 cgactcttca ataactgaca aagcccgagt tatcgggcct attttttact tactgctctt
    16201 cgccgccaag gaaataaatt cccaacggga ttgccagcaa cacagtaaag accaggctat
    16261 aaacaaaaat catcgatgac tgaatcacat acattgatgt cgtccactca gacagcggaa
    16321 tattgtactg ttctataacg ccgccgatag tcgcgcgagt cacaaccgac gccgcaataa
    16381 tgaaaaccgc caacaaacca aagagaaatt tcttcccttt cgctgacttc agtttcgcca
    16441 taatcatgat gtaatccttg tgcagacaaa tttcttgtta cctaaacgta acaataccat
    16501 gacatttcac cgttgtctgc tctgaaaacc tcacctctta gctggattta caatcaacgc
    16561 attgattagt tagctaatat tgctacttcc ttccctgact attgatgatc tgaatgaatt
    16621 gtcgcggtaa aatagccccg ccaggggttt ccggcatgac gacaattatc tggaggagcc
    16681 tgataaaaca atgatcgctg aactgtttac aaataatgcg cttaatctgg tcattatttt
    16741 cggtagctgc gcagcattga ttctgatgag cttttggttt cgccgcggaa atcgtaaaag
    16801 aaaaggtttt ttattccatg cggtgcaatt tttaatctac accataatt
//
LOCUS       DAZFRN010000106        16844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFRN010000106 DAZFRN010000000
VERSION     DAZFRN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.108"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..80
                     /locus_tag="ACHKUB_003362"
     CDS             <1..80
                     /locus_tag="ACHKUB_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO6656338.1"
                     /translation="IGFANTTFSDADFGALGILLGIIVR"
     gene            complement(296..580)
                     /locus_tag="ACHKUB_003363"
     CDS             complement(296..580)
                     /locus_tag="ACHKUB_003363"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6656339.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQVVKLALQPRVSVARIAREHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(711..3392)
                     /locus_tag="ACHKUB_003364"
     CDS             complement(711..3392)
                     /locus_tag="ACHKUB_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO6656340.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDGEVMLTCHFRSGKKYTRP
                     LSVCQITPTVILRNVFLERKGNAVTSRAERVIIYGDKYAAIYYRESERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVADERVFYARGNNKNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLMAVERAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQRLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANMIDDVLQQVKKHLNAINQVASLKAEINELSVEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKSIREKQISLNEYEKVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQQSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIRYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSDDSRFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHRELLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLSG"
     gene            complement(4066..4590)
                     /locus_tag="ACHKUB_003365"
     CDS             complement(4066..4590)
                     /locus_tag="ACHKUB_003365"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO6656341.1"
                     /translation="MAQLSRTPSLLNHASEWITLSGQQITRLTELPPAYNLQRSAQLL
                     QQLRGLFPDNPRVQEMVDNWQKSVRSRALPEEAMTGWNEGMTRLQQLAERLNRLDEQR
                     GKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLIN
                     RYQMEHTGNQERQP"
     gene            complement(4642..4791)
                     /locus_tag="ACHKUB_003366"
                     /pseudo
     CDS             complement(4642..4791)
                     /locus_tag="ACHKUB_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5116..5430)
                     /locus_tag="ACHKUB_003367"
                     /pseudo
     CDS             complement(5116..5430)
                     /locus_tag="ACHKUB_003367"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            complement(5485..5790)
                     /locus_tag="ACHKUB_003368"
     CDS             complement(5485..5790)
                     /locus_tag="ACHKUB_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656342.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            complement(5843..6292)
                     /gene="tssE"
                     /locus_tag="ACHKUB_003369"
     CDS             complement(5843..6292)
                     /gene="tssE"
                     /locus_tag="ACHKUB_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO6656343.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            complement(6295..6831)
                     /gene="tssJ"
                     /locus_tag="ACHKUB_003370"
     CDS             complement(6295..6831)
                     /gene="tssJ"
                     /locus_tag="ACHKUB_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO6656344.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEVLAGD
                     IIAQKDVWLQPGGSVAVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSLDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(6812..7912)
                     /gene="tssG"
                     /locus_tag="ACHKUB_003371"
     CDS             complement(6812..7912)
                     /gene="tssG"
                     /locus_tag="ACHKUB_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO6656345.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYPGMGFPASEIKDAVIPEESHLPPTVQVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEPGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYPGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(7867..9630)
                     /gene="tssF"
                     /locus_tag="ACHKUB_003372"
     CDS             complement(7867..9630)
                     /gene="tssF"
                     /locus_tag="ACHKUB_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO6656346.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ASSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENVSLPAGISGFDLE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVTGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEADRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPVSVHNLCKPTL
                     PVYPPTEDCFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(9638..10330)
                     /locus_tag="ACHKUB_003373"
     CDS             complement(9638..10330)
                     /locus_tag="ACHKUB_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO6656347.1"
                     /translation="MSVMHYLQDKTFQDIVKNLVLPLLSGIIIPLVKWFVQHYGYAPN
                     IRKYRFEKIPVSEKESILARIDKLTKEPTTKNTLVRIKYCYEQMGIYLPIWCCNKLIC
                     FISDRNVSSVDNRLHCFLKYSFVGMFSDGKFTVNTRRVHKGYRMIAVFAVFSLCVQFT
                     GGIFTTMPFLSGGNTVLFMLFSLVYFIMIFLTVIFTCNLINEIRLAVQFGRLFEAWLK
                     SERESPEQLALF"
     gene            complement(10332..11930)
                     /gene="tssA"
                     /locus_tag="ACHKUB_003374"
     CDS             complement(10332..11930)
                     /gene="tssA"
                     /locus_tag="ACHKUB_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO6656348.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKDIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLAELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNPGSS"
     gene            complement(11930..15319)
                     /locus_tag="ACHKUB_003375"
     CDS             complement(11930..15319)
                     /locus_tag="ACHKUB_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO6656349.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTPPRYVTVQDI
                     RHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGEPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPLDSPQRGKMAKAAYDQLRLYLMLTQPQHM
                     EPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAASGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(15312..16451)
                     /locus_tag="ACHKUB_003376"
     CDS             complement(15312..16451)
                     /locus_tag="ACHKUB_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO6656350.1"
                     /translation="MGWKRNRISLLISPSAPSLIWWSLSGILVIIVGILLFIIHASGH
                     VKTLNMLSIWWFSLTPPGIWFLLFLLRCWQWNNQIDKYLFLKKENEYAQMQWEVWAER
                     YLVISASSVMLPGGVTAGAILKSLADTLPSGYLLTKRLKNINTPVTSALASLQLSICQ
                     LPAALPVNVTLITDQPDSEIRSAFVSAWEALFPQRVVPDNIEVTPDFSMGWVDERLKQ
                     PVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINKFND
                     EFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWCGIP
                     GPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(16454..16720)
                     /locus_tag="ACHKUB_003377"
     CDS             complement(16454..16720)
                     /locus_tag="ACHKUB_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO6656351.1"
                     /translation="MVKGVVLFGDKTTHGGQVISASSNITVNGKKVALVGDMVSCPVS
                     GHGVNPILEGMPQRTCNGRSVVADGCKCQCGCQVISSAINSTIG"
BASE COUNT         4359 a         4567 c         3787 g         4131 t
ORIGIN      
        1 acattggttt tgctaacacc accttcagtg atgctgactt tggcgcactg ggtattttgt
       61 tagggattat cgttcgctaa tattttgatg acattaatat cctccaggat ttcaggaacg
      121 tggaggatat taattcctct tattttctcc atttttcttt cttccagtca ggactgcatc
      181 tgattaatcg taagcgctcc gggaagtacg tcgcgtaagg attattttac agctgagaag
      241 tttcagtgca gcagttcatg cacacggttc gacggctagt cattgatctt cataattacc
      301 tcgcgtaaaa aacggggtaa ggccgctcat ccagcgtcat ctccacaggt aataattcaa
      361 cgccaatgcc agaagagttc cttaccggaa gacgccgtga tatacgccct ttgatcagcc
      421 agagcctgag ccatttgaac agaagattat cattgatatc atgctcccgg gcaatacggg
      481 caacagaaac ccttggttgt aaagccagtt taaccacttg aagtttaaac tcatttgaaa
      541 atgacctgcg gggttcagct gataatgttt tttgttccat aacaggcatc cactagtttg
      601 aaaaggtggg cacctacgtt accaatactg gcttaatggc tacatacgac ggtcggttta
      661 cgcttaccag aaaactaata gtgctatatg caatttttaa tattcagact ttagcctgac
      721 aggcctgaca aaaacgcgcc caacttccct tctatatcac tgtctatagt acgtaatctt
      781 agcaatggca gaccacattt cttcagtata ctgtttttaa gttcatcacg ttcagcctgg
      841 acactggtca gatgatagcc tccatccact tcaataaccc ctaatggagt cttcccgacc
      901 ttatagtaaa caacaaagtc acaactggcc cgattacgca tgaataacag ttcacggtga
      961 gtaaaatctc cccgctccag caacaccaac tgattcagcg ctatttgtga atgaaacatc
     1021 atgctccgat aactatcctg cgacaggata tcccgcaaca ggcaggccac aatctgttct
     1081 gacttaaagc gtgaatcatc tgaattcaat ctactattca gtcgttccag tgatttgtca
     1141 tactcactgt acagcaaatc aaatgctgat atcaccgggc tctcgaaaat ctctccgtca
     1201 tcagcgtagt atctgatgta acgaatcagt gccgcaatat gtccagcgtg gcgttcaaat
     1261 acatcattac cagtcaccaa cgtaaatttg tttcgggccc ttgaaacagc gacattcacc
     1321 agttcaggat tatcaacaaa cgcgatattc cgactttgct ggctgctgcg tttcttatcc
     1381 agcacagtgg aaaacacaat ttcatcgcac tcccgtccct gaaatttatg caccgtcgat
     1441 ttcacaaaat cagcgggcag cactgtttca gccagattaa cctgtgcatt atatggggcg
     1501 atatatcccc tgctactttc ctcatcccag taatgtcctt ccagagattc aagctccctg
     1561 agatttgaaa aattacgggt atggtttcct tttgccgtga tgatcagaga taatgaggct
     1621 tcacctgaat caagcgttag cggtatcaga gcattatcat aaaactgctt attacaaaat
     1681 tgaatgattt taggatgaca gcgataatgc tctttaagaa gagttactgg caccatattc
     1741 ctgaacaaca tacaaaccga atcaagtaga ctatatttct cacaattata gtattctgcc
     1801 ggtggagatg ggctgttcgg gagtaataca ggaacatgtg gaagttgctt tcgatcccct
     1861 acaacgatta cattacgggc acatcctagt cccagaatgc ccggaacaat atcctgctgt
     1921 gaggcttcat caataatcac ataatcaaga agagccccct taccgataga gttaataata
     1981 gaatgcgtgc tgctaccaat aaccggaaaa cgctttataa aacggttaaa ttcatccctg
     2041 tatgtctctg ccgagaatga atttcttgtt gaaatatttc ttcgcagatg ttgttttaaa
     2101 taaagcacag accatgatgt caggcgctcc agtaaaattt taaaatcaga ttcctttaat
     2161 actttctcat attcattcaa agaaatttgt ttttcccgga tggatttttc ataataagaa
     2221 agctgaagag aagtaaatag tgccagcctt ctctccggaa tcaccaatgg tttgactttc
     2281 agtatcctga agtttaacaa caaatcaatt ctgtttctga atccaatccg cctgtcagaa
     2341 aggtaatgaa tataagccat caggtcagtt gtttttcgtg aggaaagtcg gtatttgtga
     2401 gaaaaaagct cctccggtcg aagattctga ctctgccatt gctgcaggta tttatactca
     2461 acactcagtt cattaatttc tgcttttaac gaagcaacct ggttgatagc attcaggtgt
     2521 tttttcactt gttgaagtac atcatcaatc atatttgcag acggtgagtc aggtaagact
     2581 tcctcagaac gcgaaatcga cgttgaaaaa aactgttgtc tgttctccgt acttcccagt
     2641 cgtgccacca catatcccag tcgttgtttg tccatttttt cataaacatt actgacagct
     2701 gagttattat tggacaaaat tgccactgtt ttattctgaa ttaatatatt ggcaacaata
     2761 ttgagaatag tctgtgtttt acctgtccca ggtggtcctt caataactga aatctgtgag
     2821 gaaaatgccc gttcaaccgc cattaactga ctctcattca ggccaaacgg aaaaatcaac
     2881 ccccatggcg aatcacgttt tttactctgt cctgaacagt atgcatgcaa ggcggtatcc
     2941 ggatgaggaa ctattttctt tatctgtctg aggatattct catcgatatt tttattatta
     3001 cctcgagcat aaaaaacacg ttcgtcagcc accctgcaca agtaattaaa cacggcatgc
     3061 tctgtaaatg ctgaacactg ttgaaagtca agtccggtcg ttttcatgat gtagggtcgt
     3121 tcgctctcac ggtaataaat agcagcatat ttatcaccat agataataac ccgttcagcc
     3181 cgggatgtta cagcgttccc tttcctttcg aggaaaacat ttcgcaaaat tacagttggc
     3241 gttatctgac atacagaaag cggacgagta tattttttcc cgctgcgaaa atgacaggtc
     3301 agcataacct ctccgtcatc atttccagcc cacagagtcc agtcggtaat tttatgagtc
     3361 ttattttcac cgtcaacaca aatcgtgacc atattctcac tcagtaatta tcatttaact
     3421 tacggaaaat tgttatgaaa ctcccgatat ttacccggta aaatacttgt cattgtttga
     3481 acccctccct aaaaaatgac caggcatttg ataacccggt gtatttctcc cgatatgagc
     3541 tccggaggat tttccacaac tccgggtgtg ttaacatatt cccgccggcc atatatacat
     3601 tgagttcagt cataacagtc aataccagga ttacaggaac gcgatgctga tacgctggtt
     3661 attgaatact ggtagcccct aactactaat tcatcacgat actctgtact tactgttttt
     3721 tacaaccatt cccccaggta aaagagtcca tttatcatgc ccctctttaa cgcctgccag
     3781 cataatttaa gaatggatac cgtgggtgga attaacaata cctgctcagg ccaatgccat
     3841 gtaaccgcct cactgaaaaa gccagatcat ctaaccaggt acagaccaac atcgcaaaaa
     3901 ttaccatgta gcacgaactg ctgtcatatt tctgttcagg tatgccgcta tttcgcgggt
     3961 tttcttcacg ccataatgcg tttcaaggta gcgaacatct tcgattgtcc atggtgtgcg
     4021 cgctcttccg ggggtatttt ccatttccat gttttatctc agccattatg gctgcctctc
     4081 ctgattgcct gtgtgttcca tctgataacg attaatcagc tgattcagtg ccatttcagc
     4141 ctgtttttgc tgctgttcac tgccattctg attacgggct tcatcatagc gacgtaactg
     4201 ctcttctgcc gggatatgcc ggttaaaagc ctgcatgatg ccaaacacct ccgttttcag
     4261 ttcactgacc gtcatgtatt ttccccgctg ttcatccaga cggttcaggc gctcagccag
     4321 ctgctgtaag cgggtcatgc cttcgttcca gcccgtcatc gcctcttccg gaagcgctcg
     4381 gctccttacg ctcttctgcc agttatccac catttcctga acacggggat tgtcggggaa
     4441 aagtcccctc agttgctgca gcagctgcgc actgcgttgc aggttgtatg ccggcggtag
     4501 ttctgtcagt cgcgttatct gctgaccgga aagggttatc cactctgacg catgatttaa
     4561 cagtgagggt gttctgctga gttgcgccat ctggtctgct gaaagcacat ccggtagtgg
     4621 tgccagcagc gccctgagtt gctgctccgc cggtgatggc tgtgtcagaa actgccatcc
     4681 cggaatgacc acgctgacag tcagcaatgc cgcacagaca cccgccacaa acggtttcag
     4741 aaacgtgggc ggggatttct gaagggaggg tgtgggttct gcttttgttt cagaaacaac
     4801 atacaccaga cgggagtacg gcaggggctg atgttcctgt acatccggag catcagaccg
     4861 gagtgcctga gccggaaggg ttaccggcga cgatacttca ccctgcacag gctgattctc
     4921 cagacgggtg atatatccct tcaccatggc ttccggtaaa gccactttac tgctctgttt
     4981 aagttcatgc cgggtcagta tgtcactcag cgttttcagg catgtttctg tctgatacag
     5041 caggggcagg ttgtcacgct catccggtgg catggcccgg agcgtttttt gcagacgact
     5101 gaacagaccg gtaataattt ccagccgggc gtgggtattc agcggccaca tcactggcca
     5161 gtgatgccgg gtcagcgcca caatcagagc cagcccttca ttcaggccac ttaaccctgt
     5221 tgtatgcatc cgggcgatgg tgtaccagga tgccgtctgt aattccacgc cattgtgttc
     5281 atacagacgc aggcagagcg tctccacata acgccagtcc acatccggac gtgccgggtg
     5341 agtcagcttg ctgatttcat cgcgcagggc agcataatcc ggtaatgacc gggggtcatt
     5401 acctgttttc agttttactt cagaagtcat cggcagattc agtgttgctg tatcaaatca
     5461 taattaatat tcccggaaag agcgtcagtg tgataccggt acccgggaaa ggtattccac
     5521 cgcacggatg attgccggtg tcacatccca ccgccgcttc agaaccgcca ataactcact
     5581 gagtgttctg atataacgtt cagtgaacag tcgctgcccg tccctggtta aatgaaaacc
     5641 ggactgatta caggtaatgg taatatggca ggcatcatta ccaatatata cggcttcctg
     5701 tggcctgaat ctgacattcc agtgccagga atacggtgca tgattcagat aaaaaaggcg
     5761 catctggatg tcattaaata acgggttcat cattcagccc tcgtttatgt ctgttaagca
     5821 cacggataag gggaaccata aatcaccccc tgaaagtcat cacattatcc acaaactgct
     5881 gttgcttcag atatcgcagt aacaccctgc cctccggcat aaaagttgtt ccgtaacgaa
     5941 ccagcccggc atctttaagt tctgcatcaa tgacataatg caactcacca ggctgagtct
     6001 gctcctgcat cgtcacgttg acacgcttaa tacgtggctc atacttcagc agaacatctg
     6061 aaagaacacg catcagttga tgcgccgttc ccggcattcc ctgcagaacg gttgtcatat
     6121 ccggcagacc ataatccggc agatgtttaa ggcttccggc ccgggtattc agaatacgct
     6181 gcatgttatc gagcaccgaa agaataacct gctcttccgc actgacctgg tttaactcca
     6241 gcccgccagc gaaattgcca tagagaattt cataaaggga aggacgcggc atcactattt
     6301 atccttcacc ggtaacagcg tcagggtatt accggatact tcaataagac ggggcgtgtc
     6361 cggctcaagc tcatccaggc tcagcaccac ccgccaggta tttttcttcc ggtcaggctc
     6421 cagaaacatt gctgccacac cggtaaattt cgccgcatca tccagaggca tatccacggc
     6481 cactgaacca cctggctgca gccatacatc tttctgtgca atgatatcac ctgccagaac
     6541 ttcactgtcc cccgtaaaaa gcgcctgata atctgcactg tcaaaattcc ggttgtcttt
     6601 aagctgataa atccgcacca caaccgacag gggaataccg gcagcactgg tgttaatggc
     6661 ctccctggca cgaatatcaa ggtgaagtgt ttttatctgt cggtaaaaca gggatttcgt
     6721 ggcagatacc gtaccgtctg ccactctttg cgtcaggcca caccctgata acgacaggga
     6781 aaacaacgca ataatcagac cgtaaccagc cttaccagcg ataatcgcca ttttcctgag
     6841 cctccttacg ttgataatgt tcctgaaccc gctgataacg ccccagacgg atggtcatta
     6901 cgcgtgccgc tgaagtcgtt atctttgcct gtgttctcat tacagccgtg cgtcccagtt
     6961 gcggactacc ggctgccggt cggcaactga gccggacatc cggcaacagt gaacgctcaa
     7021 cacaaagttg cagacgtaca tccagacggc ttcccggata aacgtgcaac aacgccagca
     7081 aatcagaata cagctgcccg cccggtaacc agccctgtac ctcgctgccc gtctgtgttg
     7141 aaagctgtaa taacacctgt ccgttcacat ccgtagcatg atcgcccatc accgggcgac
     7201 tcttcagact gaccggatga tgaacacgca tggccagcgg tgttttcagc gggatccgtc
     7261 gtcggtcatg atgccagaca cgggcctgtg ttccgggagc cagcagcgtt accagtgatg
     7321 tcagcccttc agctgttctt ccgggtaaca acatcagggg caataacgcc agaaaacgcg
     7381 ataccggcgt ggcaatattc tgtgcacatc cggggatccc cagtctggcc aggccaagca
     7441 gatactgaga cgttttatcc tgcccacccg gctcaaaggt ggccgggtac gaatacttac
     7501 gccagatacg gtaatactgg gtgatcagac gatgactgaa tatatcgaga aaatccgctg
     7561 cggcctcatg gccttcccgc tgctgtgcaa tatcactgat atagtgtgcc ggcaacggag
     7621 acgtgacccc gtaaagtccc ataaacgtca cctgtacagt gggtggcaga tgggattctt
     7681 caggaattac agcgtctttt atttcactcg caggaaaccc cattcccggg tatggacaaa
     7741 agcggaccgc ttcctgacgg gcctgccagc cactgccaat gggaggctgg tctggctggc
     7801 tttgctccag caactgacag aagcggtaaa aattaatata cggaagctgt ttccggtaac
     7861 gggtaattaa ccgggcaggc gcggattgtg attttctttc caccggatgc attttccttc
     7921 aggctgaaca atcagtgtga gctgattgaa ctgattcata tcggcgtaca gggccaggaa
     7981 acggttgagc atttcgccaa acaggtgaat atctccctct ccggcaaacc cgttgccgtc
     8041 aagagtcacc tccacatcaa gcccccggag cagaaaaccc ttttcaaaac gctgaatacg
     8101 atgatgctgt acagccagaa tggcatcaag ccgacggtga ttaagttcat cgtcccgcca
     8161 gttataaagc gccagcgtgc cacgcagcac ttccgcactg ctcagcatgt taagaaatcc
     8221 tgtccccaga tgactcatca cccgccagtg aaaacaatct tctgtcggcg gataaaccgg
     8281 tagcgtgggt ttacaaagat tatgtacact gaccggggtc tgcagtacct gttcacaccg
     8341 atcaagcagc gtactctgca acgcacgacg gggaagctga ccattggtac ctgtaatacg
     8401 taatgaaagc gtttcccgct ccggcatccg atccgcctcc caccgctgac cgccaaggat
     8461 aagccatgtg tcatacatcc ccgtgacacc ccgtttcacc cgggtatgat aataacgttc
     8521 gggtgcatca tggcgcagca ttcccccacg gtgacggaag ctggtaaaag gcacgtactc
     8581 tgcactgccg gtccgcccgg agcccgtcac cgcatccact gaatatatct ccgtataccc
     8641 atcctgcaga cgcttcgggc gcagcaggta ttcgctctcc agcccattga tgatgagtgg
     8701 atcggcttca agcgtaaaaa ggttaatcac cggcacacaa tgcagacaca gggcatcgtc
     8761 tgtcacaggt aaatctgccg gccaggactg actcagcacc acctcaagat cgaatccgga
     8821 aatacctgcc ggcagagaaa cattctccag tccgttcaga tgaacaaaca taaacttttc
     8881 ccgaaaggtg aaatattcca gcaataactg atacccactg aaagcactgt cacctttcgg
     8941 ccacagccga tcttcttctg caaaaccacc gggtgaaaac catccgtcaa tgcggatccg
     9001 ctcatcattt cccggcagcc tcagatacag cgcagccaga cgctttgtca tcatcagatg
     9061 aagggtgctg ctgaccgggg cttctgctgc cagatacagg ctcagacgat gcaaatcgac
     9121 ctgagaccag tcagccagtt cactgctggc aaaacctatt ttcagcactg agcgcccgtc
     9181 cggctccgta gtcataacga ccttctccac tgccagtgga ttcagtggta tggcccgggt
     9241 ggtacggtaa cgacagactg tatttccggg gccaacaggg cgggaagtca cttccagtcc
     9301 ggctggtacg gtttcagcca ttttcatgac ggataaccgg ggagtcagtg ccaccaccga
     9361 cagggacggg atagtacgca ggtaatgagg ccagagcata ctgaccagac tttcagtaag
     9421 ctccggcaaa tcatcatcga ttttctggcg cagccgtccc atggaaaagg cgaaaccttc
     9481 aaacaggcgt tccacgcagg gatcaggcgt acccgcttta tcgagatcca gcatcgccgc
     9541 ccgatcggga tgagcctgtg caaacgcctt tccggcttca cgcagataac gcatttcagc
     9601 gtcaaaataa cgcagggtta aatcgtccat tatcttctca gaataaagct aactgttccg
     9661 gagactcgcg ctctgacttc agccatgcct caaacaatcg cccgaactga acggcaagcc
     9721 ggatttcatt aatcagatta catgtgaata taactgtcag aaatatcatg ataaaataaa
     9781 cgagcgaaaa taacataaat aaaactgtat taccccccga cagaaatggc atggttgtaa
     9841 atatccctcc cgtaaactga acacataaag aaaacaccgc aaacactgct atcatccggt
     9901 aacctttatg cacacgacga gtattaacag taaatttgcc atctgaaaac ataccgacaa
     9961 aagaatattt aagaaaacaa tgcagtctgt tatccactga actgacattt ctgtcggata
    10021 taaaacaaat cagcttatta caacaccata tcggaagata tatccccatc tgttcataac
    10081 agtattttat tcttaccagt gtatttttcg ttgttggctc ttttgtcagc ttgtcaatac
    10141 gggcaagaat ggactccttt tcagatacag gaattttctc aaatctgtat ttcctgatat
    10201 tcggcgcata gccataatgc tgcacaaacc atttgacaag aggaataata atgcccgaca
    10261 gaagcggtaa caccagattt ttaactatat cctgaaatgt tttgtcctga agataatgca
    10321 tgacagacat ctcatgagct ccccggattg cacaacacag cagctctggc agcatcaatg
    10381 gcgatgagcc cggcaagaag atgttccata tcaggctgca gacgcaccct gtcactttca
    10441 gtttttgcgg acttcatacg taacagtttc aggcggcggg cttttacctc aaataccagt
    10501 tcaggttccc attgtgacag ggtcagccgg gttgcacgtt catccagctc cgcaagcaga
    10561 tgcagggcaa ggtcattttt tccagtctgt tcggcaacac gggccatcaa caggcgcagt
    10621 agccagttgc tgcggtcaga ctgaataccg ggacgtgcct gtaaccagct taaagcggct
    10681 tctgttcctt cattatccgc aatctgcagg gcttccggct caagagataa aatgtcgtta
    10741 tctccggggg ctgccgttat cgttacagac ggttcttccg cccagcgttc accacactcc
    10801 atcacctgct gctgtatcca gcttagcgtc acgtcatcgg caaacggcat gccgtcgtta
    10861 aaagccagtg tctcaagccc cggcaggcgc agaagcaatc ctttcaggtc atactgaata
    10921 atggcagcat aattttcttt tccggtctgc aacagcgcct gatgaatata ccactgcagg
    10981 tccagccaca gatgactggc tccccgggca aacatatcgt cacactgttc aagcagtgac
    11041 aaccagttct gctgcagata caaccgtttc agtgacgcac gccggtccgg ccccggtggg
    11101 gcaatacggg tccgtccatc agcagataac ggcggtaact gatgcagcgt gtcatgccgg
    11161 acgcttttca tcagtcgatg agccgccagc cagccctcag gctggtcacg cagatatttt
    11221 gccagaacac gggcctgtga cagcagttcc tgtccggagg tgatggcaga taatgccatg
    11281 acgctgccgg atgttaccgt cggtgccggc gcggcagcct cctgaggcac cactgcatca
    11341 acaccaccgg ccttcatcag gcgattttca agtgcctgat ataacccgtt cagcaccggg
    11401 gcggaagcct catccgtaaa ggtctgttct gccagccaca gcgccccggc aatgcgcagg
    11461 gtatccgcct tcaccacctc cggatacaga gacagactgt ccagtatgcg ggcgctgcat
    11521 aaccactcca gcgccgcctt cctggcccgt gaccgttgtg gatgcaggtg ctctccaaaa
    11581 cgctgcaaca aaccggtcag cagctccaga ccgtcggcca gccctttttc accatcctgc
    11641 cgcagacggg cccagatata ccaggtcgcc acccggatat ctttggttct ggtggtcagt
    11701 aatttttctg caagctggct gacaattccg gcatcggctc cggagagttt attgacctct
    11761 tcacgcatct gctgaaaatc atcatcgtac cccgcatcat cacctgcggg attatctggc
    11821 gtcacgggag caagccattt atcccacagc gcaatacgca cctgagccgg ttcagccaca
    11881 tcccggggtg tcgtctggca tgcactgagg agcgaatgaa tggaagccat cagtcagtct
    11941 cctccacgga atccccggca tcttcgtcat tgcctgtaaa cgctgacgta ccggagagtt
    12001 cgaaaaccgt ttccggcaga acaaaattgc ggagtttcag caatacaagc ggcccttccc
    12061 ccgcttcagt tcgcagtgtg taattcagca tacgaccgtc ctgcgcctgc cagctcaggc
    12121 tccagccact ggcaacgccc gatgcggctt tccgtcgggc catctcaagc agacgtatca
    12181 gcccccagct tcccggcagg tctgcatact gacgggtacc ggcctgagtg cttacccagc
    12241 ttaaactggc gccaggtgct tccgtatcag ccggccaggt aaatcgcttc cataccggca
    12301 tctgattaac ataaatcagt ttctgattat cggttatcag cgtcgtctgc atcacaccgg
    12361 cagcagttcc cgggcgcagc tcaaaatgca gccccgcgtt acctgtggtg aacgccacat
    12421 ccgctatttc actcagggta ttgatggctt tcagaaacgc agggttgaat gtcagtcccc
    12481 gggtattaat ggtatcaggt acccagcgac tgccttcact gtgcaacaca ccactcaggt
    12541 tattctgcag gaaacgggca atacgtcccg tgtccgtatt caggtatttt gccagcaggg
    12601 gaagcgaagc atcgctgctg acatttttaa acggataacg cccggaaaat gcattattcc
    12661 agccatccac caccgcagta cgccaccggg cattcagact ttctgccgcg ggcgtaagaa
    12721 cctgctgcca cgcctgctcc atcgggcgaa caaagaccgt ctgtccgaag ccataccact
    12781 cctgtccgag tccggcagcg gtcaggctac cgtaatcccg ggtatcggtt aaatcaaccg
    12841 atttcccctg caatactgtc tgtgccagca tctgcatcat ggcctgcggg tcagaagagc
    12901 cggcaatctg ttgcagacgc agacgaacct gtgtcacccg ggtcagatat gtctgcagat
    12961 tcagcatatc cgcactgttg ttctgattat ccatcagtgc aagcaccggc ccgaaagtcg
    13021 tcgccagagg accgtgaagc cggctttccg gtactgccac tggctgtttt tcctgtgaaa
    13081 gcaggtttct ggcagattta accagtgaat cagtaaccgc ttcccggggc tgaccagtac
    13141 gcccctgaac cgccagcgta ttcatcagtg caaccagggg ggactggcgc acatcagcca
    13201 tcagcgtcag ttgctctgtc acatcagaca gcgtctgagc tttacgcaag tgcagactgt
    13261 tcagaaaatt cagccaggca ttaccaaaat cagcaaaata gcgcgttgtc aggcgctgtc
    13321 tgagtgcttc cggcgatacc ggtgacggtg ccttctgcct gccatccgtc agcacccagt
    13381 ccatttcttc acgacgctca ttaatcacgg tgtctatcga cggcaatacg gcctcttccc
    13441 aggcctggcg ggtaaacata ccgggaacca cttcatccgt aaaaaagagg cgggagacat
    13501 cagtatcacc ggtcatatcc gtcagacgca tatccgcaaa ctgatgtgcc acccgtgcca
    13561 gcattttctg atacagcatg gcatcgctgt tctgtgttcc cagatggcga agcagcagag
    13621 ttctgctctg gctgaccagc tcttcatcag ccgtcagctt ccactgagga tgagtaataa
    13681 tcccggaggc ataatacgcc agcaacgtcg ggccattagc ctgccagaaa acggcactca
    13741 caccgtcacg ctgtggccat tcccgcatga gcgtacgact gaaccaggcg ggctccatat
    13801 gttgcggttg cgtcagcatc agatacagtc tgagctggtc atatgcggct tttgccattt
    13861 tcccccgctg cgggctgtcc agaggaagac gaataaatgt cctgagctgc tgctcaagat
    13921 gagcagcagc ggcatcacgc agtaaaagat gtgcattttg tgcataaagg ggcattacga
    13981 ccgcgagcag gtctgcattc tgattcatac cggcccgcag gtaccatggc gccccgtgct
    14041 cctcccggta ctgaaggcgg gccagggatt tctgcagatt cagcaacgcc tgcagacgtt
    14101 ctgcaggaga cagttgctgg ttctgagccg gacgaatctg ttcctgtact tcagcaacca
    14161 gactccggtt tgccagaaag gaaaccacca ttcctgttcc ctgagccatc atcagtatgg
    14221 ccgccataac ggccagactt ctgcgccagt tatgacctgt gcgggaaggc tgtagccgga
    14281 caggtaattg ctgcactgtc tccggcaacg cctcccagcg gttatccatt cgccagcgat
    14341 gtctgacact tcgttcaccg ccgacagttg ccggactgaa aacaataccc gccagcaaca
    14401 actgacggta tggacggagt aatacggata atggctccac cacagcatca atatttcgtc
    14461 tgaatcgctc tgccagcgac aacaaaaaat aataccgagg ggcacagcat atctgctgta
    14521 tcccctgagc aacaagcccg gggagcatgg cctgtagttg tgctttcagt ttgtcagaac
    14581 tacattctgc tggcagaagg catcccacgg gctgagtaat acgccctcgc tcatccggac
    14641 tctcctgcag tgaccagaca tacagcggca gacgccagcc aaggcgctca caacatgaac
    14701 tgaccgcacg ggaaaccctg tcaagggcat cttctgtcag tggtgcggat aatgtttcac
    14761 tcagtccgga agttacccag acaatgccat cagcaggccg gtaacgcaga cggcgtaacg
    14821 cagccagcca gtctgcgttt tccggctgcg agggctctcc tccccagaga agcaatgtcc
    14881 cctgctcttc ctgccagaac tgttctgtca ggcccggggt cagcaactca acctcagagg
    14941 cagtacctgt tatcaacaga atgcgtattt tacgtcccca gaatctgcca tactggcgcc
    15001 gcagagtatg acgaatatcc tgaacagtaa catagcgcgg tggcgtctga gccagtcgct
    15061 cttcatcgtc aggtaacctt tgctccttac gggcccgacg tcgatatgtc agttcctgaa
    15121 catatttttt catcacaggc aggtaacaga caaagagcaa ccccagactc agcaatatca
    15181 gcagtagtat ttgatcccgg cgtgtttctg cccccagaaa tgctccatat cgggttatca
    15241 gccaccagag cgcagccaca ccaataaata caatccccgg tcgtccaaag cgaccagaca
    15301 gacaggccag tttattcatt ctggtttcct gatgtaacag tactgataaa atgttcttct
    15361 ccggatgaga ataaagtcag gagagaatca ttaccaagac agaccagatc ggctgccaga
    15421 gcagtaagca gccacggagc agcaggtccc gggataccgc accatttttc cagtaacata
    15481 cgtccggacg gttcccatcc ggcaccatac tggtttccaa tagtcatcag cggtgcagca
    15541 atcttttccc agtgataaca gtcccccagt acacgagcgg tacgacaggc agctgtctgg
    15601 gtttcaagga aaagtgtgaa ttcatcatta aacttgttga tatccagcga catcgggcgt
    15661 agcagacggg ctggatgcgg caggttatat ttttgcgcaa catcatcgct ggtcaacaaa
    15721 agtgcagcca gtccgtcaga gtatgcattt cccccattaa gctgtattac cagtatcaaa
    15781 tccacagtca gcactggttg tttcagacgc tcatcaaccc accccatgga aaagtcggga
    15841 gtaacctcga tattgtcagg aacgacgcgt tggggaaata acgcttccca tgcagaaaca
    15901 aaagcagatc tgatttcaga atcaggctga tcggttatca gcgtcacatt tacagggaga
    15961 gcggcaggta actgacaaat agatagttgc aagcttgcca gggcagaagt tacaggtgta
    16021 ttaatattct ttaagcgttt tgtcagcaag tacccggaag gtaatgtatc tgccagtgat
    16081 ttcagaattg ctccggctgt cacaccacca ggaagcataa cactgcttgc ggagataacc
    16141 agatacctct ctgcccagac ctcccactgc atctgagcat attcattttc ttttttcaga
    16201 aaaagatatt tatcaatctg attgttccac tgccaacaac gtaaaagaaa taacaaaaac
    16261 cagattccag gtggagttaa tgaaaaccac cagatactta acatattcaa cgttttcaca
    16321 tgcccagaag catgaataat aaacaatagg ataccaacaa taatcactaa tataccgctc
    16381 agtgaccacc atatcaggga aggagcagat ggtgatatta aaagagatat ccgatttctt
    16441 ttccagccca taattaacct atcgtgctat tgatagcact ggaaataacc tgacaaccgc
    16501 actgacactt acagccatca gcaacaacag atcgtccatt gcaggtcctc tgtggcattc
    16561 cttccagtat aggattaacg ccatgtccgg atacaggaca gctaaccatg tcccctacaa
    16621 gggcaacctt cttaccatta acagtaatat ttgatgaagc agaaattacc tgtccgccat
    16681 gtgttgtttt atcaccaaac aatacaaccc ccttaaccat acaccctcct atattttcat
    16741 cgaacaaatt tattacaaaa agcttccgca catctctatt taagacaaac tttttcttgc
    16801 ttaatgtaac taaaaccaca accagcaaca gaaaataata tcaa
//
LOCUS       DAZFRN010000107        16710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFRN010000107 DAZFRN010000000
VERSION     DAZFRN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.109"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1299
                     /locus_tag="ACHKUB_003378"
     CDS             <1..1299
                     /locus_tag="ACHKUB_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6656352.1"
                     /translation="FLFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTY
                     SGSQQNTGASDTTAQPEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDP
                     NDPKWVKGIETRDSVNQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVC
                     TACGQSGGGNCPDCGAVMGDATYLETFNEENQVEVQENDPEEMEGVEYPHKENAGGNQ
                     HHTSDNEAGGTANPLITMNGHHKITSTSRMWHHMMIDLETMGKNPDAPLISIGAIFFD
                     PQTGDMGPEFSKTIDMDTAGGVIDRGTIKWWLKQSREAQSAILTDEIPLDDALLQLRE
                     FIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAMDF
                     DARAAIPFEGERHNALDDARYQAKYVSTIWQKLIPNQADF"
     gene            1364..1612
                     /locus_tag="ACHKUB_003379"
     CDS             1364..1612
                     /locus_tag="ACHKUB_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6656353.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            1590..2720
                     /locus_tag="ACHKUB_003380"
     CDS             1590..2720
                     /locus_tag="ACHKUB_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6656354.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(2766..3404)
                     /gene="ompW"
                     /locus_tag="ACHKUB_003381"
     CDS             complement(2766..3404)
                     /gene="ompW"
                     /locus_tag="ACHKUB_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO6656355.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            3644..3733
                     /locus_tag="ACHKUB_003382"
                     /pseudo
     CDS             3644..3733
                     /locus_tag="ACHKUB_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3761..4504
                     /gene="yciC"
                     /locus_tag="ACHKUB_003383"
     CDS             3761..4504
                     /gene="yciC"
                     /locus_tag="ACHKUB_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO6656356.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            4534..5073
                     /gene="yciB"
                     /locus_tag="ACHKUB_003384"
     CDS             4534..5073
                     /gene="yciB"
                     /locus_tag="ACHKUB_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO6656357.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            5178..5576
                     /gene="yciA"
                     /locus_tag="ACHKUB_003385"
     CDS             5178..5576
                     /gene="yciA"
                     /locus_tag="ACHKUB_003385"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO6656358.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(5616..6335)
                     /gene="tonB"
                     /locus_tag="ACHKUB_003386"
     CDS             complement(5616..6335)
                     /gene="tonB"
                     /locus_tag="ACHKUB_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO6656359.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            6559..6855
                     /gene="yciI"
                     /locus_tag="ACHKUB_003387"
     CDS             6559..6855
                     /gene="yciI"
                     /locus_tag="ACHKUB_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO6656360.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            7154..8407
                     /gene="kch"
                     /locus_tag="ACHKUB_003388"
     CDS             7154..8407
                     /gene="kch"
                     /locus_tag="ACHKUB_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO6656361.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(8462..8635)
                     /gene="yciY"
                     /locus_tag="ACHKUB_003389"
     CDS             complement(8462..8635)
                     /gene="yciY"
                     /locus_tag="ACHKUB_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO6656362.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            8778..10238
                     /gene="cls"
                     /locus_tag="ACHKUB_003390"
     CDS             8778..10238
                     /gene="cls"
                     /locus_tag="ACHKUB_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO6656363.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            10273..10602
                     /gene="yciU"
                     /locus_tag="ACHKUB_003391"
     CDS             10273..10602
                     /gene="yciU"
                     /locus_tag="ACHKUB_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO6656364.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10655..11659)
                     /gene="oppF"
                     /locus_tag="ACHKUB_003392"
     CDS             complement(10655..11659)
                     /gene="oppF"
                     /locus_tag="ACHKUB_003392"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO6656365.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(11656..12669)
                     /gene="oppD"
                     /locus_tag="ACHKUB_003393"
     CDS             complement(11656..12669)
                     /gene="oppD"
                     /locus_tag="ACHKUB_003393"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO6656366.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(12681..13589)
                     /gene="oppC"
                     /locus_tag="ACHKUB_003394"
     CDS             complement(12681..13589)
                     /gene="oppC"
                     /locus_tag="ACHKUB_003394"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO6656367.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(13604..14524)
                     /gene="oppB"
                     /locus_tag="ACHKUB_003395"
     CDS             complement(13604..14524)
                     /gene="oppB"
                     /locus_tag="ACHKUB_003395"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO6656368.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(14610..16241)
                     /gene="oppA"
                     /locus_tag="ACHKUB_003396"
     CDS             complement(14610..16241)
                     /gene="oppA"
                     /locus_tag="ACHKUB_003396"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO6656369.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         4349 a         4065 c         3968 g         4328 t
ORIGIN      
        1 ttcctcttca ccgacaagga tggcgaaaaa tacggtcgcg caaacaaact ttctggtctg
       61 gaaaaagcac tagccttggg agctacggaa atcacaaaag aggaatactt cgcacgtaaa
      121 aacggcacgt actcaggttc acaacaaaat actggtgcat ctgacacgac cgcacaacca
      181 gagccggtaa aagttaccgc tgacgaagta aacaaaatta tgcaggcagc caatatcagc
      241 cagcctgacg ccgataagtt gcttgctgcc tctcgcggag aatttgttgc agggattagc
      301 gacccgaatg atccgaaatg ggtaaagggg attgaaaccc gcgattctgt aaaccagaac
      361 cagcaagaat cggaacagaa cgaccagaaa gcggaacaaa acagcccaaa tacgcaacaa
      421 aacgagccag aaacgaaaca acctgagcca gtagcgcaac aggaaccgga aaaagtctgc
      481 accgcctgcg gtcaaagcgg tggcggcaac tgccctgatt gtggcgcggt gatgggtgac
      541 gcaacatacc tggaaacatt caatgaagag aatcaggttg aagttcagga aaatgatccg
      601 gaggaaatgg aaggtgttga atatccgcac aaagagaacg ctggcggtaa tcagcatcac
      661 accagcgata atgaagctgg cgggacggca aatcccttaa ttaccatgaa cggtcatcat
      721 aaaatcacat ccaccagcag aatgtggcac cacatgatga tcgaccttga aaccatggga
      781 aaaaatcccg atgccccgct tatctcaata ggtgcaatat ttttcgatcc gcaaaccgga
      841 gatatggggc cggaatttag taagaccatc gatatggata ctgctggcgg agtcattgat
      901 cgtggcacca ttaaatggtg gcttaagcaa tcacgggagg cgcaatctgc cattctgacc
      961 gatgaaatcc cgttagatga tgcactgctg caattgcggg aatttatcga cgaaaactcc
     1021 ggcgaatttt ttgttcaggt ctggggaaat ggagccaact tcgacaacac gattttgcgc
     1081 cgttcatacg aacggcaggg gatcccctgc ccgtggcgtt actacaacga tcgcgatgta
     1141 cgcacaatcg ttgagctggg aaaagccatg gacttcgatg ccagggcggc tattccattc
     1201 gaaggtgagc gccacaatgc gctggatgat gcccgttacc aggcaaaata cgtttcaact
     1261 atctggcaaa aactgatccc gaatcaggct gatttttaat gttcaacccc ggtcgttgcc
     1321 caccagctat agtggcggcg accatgatta gcgaacgacg ctcatggcaa gacttattct
     1381 gctcactgag tgggcaaaag aggaattcag tgaaccggtc cctactccga gtacgttaag
     1441 taaatacgct aaagccggaa tgatatttcc tctccccaaa aaagttggaa gacgctggcg
     1501 agtggatccg caagctcgct ttgtcggaat ggtaaacaag ccggaggtga tcgccacaga
     1561 tcaccctgct ttgaagagga tactggaaga tggcgcgccc gcgaaaatat aaaaccgatg
     1621 ttccgggatt atctccgtat tttgacaaaa gaaataacaa agtttactgg cgttacaggc
     1681 atcccataac aggcaaaaat cacggtctcg gcagtattga ccagaaactg gcagaaacta
     1741 ttgcagcaga agcgaacagc cgtcttgccc ggcagcaaat ggaacaaatg ctcagtctgc
     1801 aggagaaaat tattagtgat accggcggtt catcaaccgt taccattttt ctgaataatt
     1861 acagaaaaat tcaacaggaa agatatgaaa acggcgagat caaactcaac acgctgaaac
     1921 agaaagcggc ccctctcagg gtatttgatg aacgttttgg caccagaccg ttagatgcca
     1981 taaccgtaaa agatgtggta tcagtactgg aagagtacaa ggccagagga cataacagaa
     2041 tgggacaaat tttcaggaaa gtactgatcg atgttttccg ggaagctcag caaacgggcg
     2101 atgtcccgcc aggctttaac cctgcagaat cggcaaaaaa accgcaggtg cggatatcaa
     2161 gacagcgact gacttttgat gagtggatga tgatttataa cgcagcggaa aaggatggtt
     2221 actttttaca gcgcggtatg ctgctggcac tgatgacagg ccagcgcctt tcagatattt
     2281 gcaaaatgca attttcggat atccgggatg gttatcttca tgtcgaacag caaaaaacag
     2341 gaacccggat tgccatccct ctggctctgc gttgcgataa attaaatctc accctggatg
     2401 atgtagtgtc atcctgccgc gattgcgttc ttagtccgtg gctattgcac caccatcacg
     2461 cgaaagggac agctaagcgc ggcgggatgg ttaagccagc aacattaacc gttgcattta
     2521 aaaaagcccg ggattctgtg gattacaact ggcgtgctaa tggcacccca ccctctttcc
     2581 atgagcagag atctttatca gagcgattgt tcagagagca gggggttgat accaaaattt
     2641 tgctaggcca ttcgaatcaa aaaatgaccg atatttacaa cgacgcacgc ggtaaggaat
     2701 ggaaaaaact ggtcatttga tgaccagttt tgcagagggg ttttgcagag gttttgcaga
     2761 gaaaattaaa aacgatatcc tgcggagaac ataaacaccc acggatccag tcgtaccgag
     2821 tcttttacgg tagtaacacc agatttgtac ttagccgtgg tatcgatatc catgtaccac
     2881 actgacatgt taaccaacca gtcacggtta atcagataat caacccccac ctgcccggca
     2941 gctccccagg aatctttcag actgagatcg gaaagccccg cctctttgcc atgatcgtta
     3001 aatccattat caaagaaggt ggtgtagtta ataccggccc caacgtaagg acggaatttg
     3061 ctgctggcat caccaaaata ccactgcgcc atcagtgttg gcggcagatg gtgaacggtt
     3121 gcaatatcgc cggtcgcccg ggtgccgatt ttatggcgga acggcgtcgc tgccagtaat
     3181 tccacaccaa tgttgtcggt cgccatataa gtaaacgtca agcccagttg agtgttattg
     3241 gtcacgctga atccacccag acttcctaac gtaccaccag caccttctgt tggacgtacg
     3301 gttgcagaac ctgcacgcat aaaaaattcg cctgcttcat gcgcaaaggc actgccagaa
     3361 agaagagttg ttactgccaa agccgccact gttaactttt tcatatccgc tccgtcgtta
     3421 tggttataaa aacgtgacga atatacctac attggagtaa caaatgatcc tacatagatc
     3481 acattacata tgataattca ccatttattg atctggatta atttagaagt tgcattgaga
     3541 atcctggtta atgtgattta aatcaatttt taaatatttt ctctacgcaa atattaactc
     3601 gatcgccact tgccgcggtt tgcccacgcc tgaaggccag caattacctc cgctttacaa
     3661 agatatgctt ttccatacaa gacctgatgc tgccacccga ggcttaacca gggtacaatt
     3721 gcccgctaat taacacctgc ataaaactca aggagagtgc atgtctatca cggcgcagtc
     3781 cgtataccgt gacaccggaa atttcttccg taatcaattt atgaccattc tgttggtatc
     3841 gttgctatgt gcgtttatca cagtggtgtt agggcatgtt ttctcaccca gtgatgcaca
     3901 gcttgcgcag ctcaatgacg gcgtgcccgt tagcggcagt agtgggttgt tcgacctggt
     3961 tcagaatatg tcaccggaac agcaacaaat tttgctgcag gcttcagcgg cgtccacttt
     4021 ttcaggatta atcggtaacg ccattctcgc cggaggcgta atattaatta tccagctggt
     4081 gtctgcgggt cagagagtca gtgcgctgcg agctattggt gccagtgcgc cgatattgcc
     4141 caagttattt attctgattt ttctgactac ccttttagta cagattggca tcatgctggt
     4201 ggtcgttccg ggaattatca tggccatttt actggccctg gcaccggtga tgctggtaca
     4261 ggacaaaatg ggcatttttg cctcgatgcg tagcagtatg cggctgactt gggcgaatat
     4321 gcgtctggtg gcacccgcag tactgagctg gttgctggca aaaacactgt tgctgctttt
     4381 tgcctcttct tttgccgcat taaccccgga aattggtgcc gtactggcga acaccttgag
     4441 caacctgatt tctgccatat tgctcatcta tctgttccgc ctgtatatgt tgattcgcca
     4501 ataaccttaa gcatttgatt acggaatcgt aaaatgaagc agtttcttga ttttttaccg
     4561 ctggttgtct ttttcgcgtt ttacaagatt tatgacatct atgcggctac tgcggcgctg
     4621 atcgtcgcca cggcgattgt gcttatctat agctgggttc gctttcgtaa ggttgagaag
     4681 atggccctga tcacttttgt tctggtggtc gtcttcggtg gcttgacgct gttcttccac
     4741 aatgatgagt ttattaaatg gaaggttaca gtcatttatg ctctatttgc gggagccctg
     4801 ttagtcagcc aatgggtgat gaaaaagccg ctaattcagc ggatgctggg taaagaactc
     4861 acgctgccgc aaccggtatg gtcgaagctg aatctggcct gggctgtttt ctttatcctt
     4921 tgcggtctgg caaacatcta catcgcattc tggctgccgc aaaatatttg ggtcaacttt
     4981 aaagtctttg gcctgaccgc ccttacctta atcttcacat tgttaagcgg tatctatatc
     5041 taccgccaca tgccgcagga agataaatcc taaccagact gccagtcctt cggggctggc
     5101 gctgtatctc ctctgttctc ctcaggaaaa tcatagtagc atcgcgcctg tgattttcct
     5161 tttaagtcgg ttttaccatg tctacaacac ataacgtccc tcagggcgat cttgttttac
     5221 gtactttagc catgcccgcc gataccaatg ccaatggtga catctttggt ggttggttaa
     5281 tgtcacaaat ggatattggc ggcgctattc tggcgaaaga aattgcccac ggtcgcgtag
     5341 tgactgtgcg ggttgaagga atgactttct tacggccggt tgcggtcggc gatgtggtgt
     5401 gctgctatgc acgctgtgtc cagaaaggga cgacatcggt cagcattaat attgaagtgt
     5461 gggtgaaaaa agtagcgtct gaaccaatcg ggcaacgcta taaagcgaca gaagcattat
     5521 ttaagtatgt cgcggttgat cctgaaggaa aacctcgcgc cttacctgtt gagtaatagt
     5581 caaaagcctc cggtcggagg cttttgactt tctgcttact gaatttcggt ggtgccgtta
     5641 attttaaaca ggatattcac cacaatccca ctgcctggct tacccggctc ataacgccat
     5701 ctgcgcatcg catttttcac ctcacgctca aacatgttcg caggcttggc tgagaggatt
     5761 tgtacgttat ccacgcgacc atccggcgtg acgtcaaatt taactttaac ctgcccttca
     5821 atgcgcaatg cctgtgctcg tgccggatac tgcggctgat tacggcttaa tgcgcgtggt
     5881 cctgaagcca cactggtaac cggcttgctg gttgcagccg ttgccgtact tgatgtcagg
     5941 cgcgcgggtg ccgtattttc aaacggtgat gccggacgcg actctacggg tttgatatcg
     6001 cgttttggct gctcctgtac ctttttcacc ggcttcggtt ttggcttagg tttcggcttc
     6061 ggcttttcaa tgaccaccgg tgcttctttt ggcggttcgg ggatcggctc aggttccggt
     6121 tctggctcta ccaccggctc cggtggcggc tgaacggctt gtggcggttc gagatcagca
     6181 ggcgcaacca tcgtgacaga aattggctgc gcaggcgcag gtagttcaat aacctgatgt
     6241 accgaggtat agagcagacc cgccacaaca gcaccatgaa tgcagaccga aagtaacgtc
     6301 ggccagggga agcggcgagg taaatcaagg gtcattgaag tcataatcat ttcagtagaa
     6361 aaaccaggtc tcgattttaa atgcaaatag caatcatatt caataaggca agacgatcag
     6421 gatcagtgaa aatgtcattt ttggcgtaat tgaccttact ttatgcatgg tcttaacaaa
     6481 gacatcatct ttccgttgca gatcttcgcc ctttcaaata acgtacttta caactttccc
     6541 gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc aagacaaagc tgatagcctc
     6601 gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt tacagttact gcatgatgaa
     6661 ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca gtaacgatcc aggggctgcg
     6721 ggatttactg gctcaaccgt tattgctgaa tttgaatcgc tggaagcagc tcaggcctgg
     6781 gccgatgctg acccgtacgt agcggcaggt gtctatgaac acgtttcagt gaaaccattt
     6841 aagaaagtgt tctgaataga gattcaatat ttaagggcac ggtttttgca accgtgttct
     6901 tgtttggcct ttccttatag ccggtcagtg cattcactac cacaagcaga taataacttt
     6961 atctgtattt ctttatatga tgtgaacata ttcaggaaaa tgccctttcc tgacgcagag
     7021 ttgctcgctc aaaacctttg attactgccg ataaagtcat ctactattgt ttaacacatt
     7081 cacgcgctgc atgtatgttc agaaaatgtg cacgctaaga taattaaaca aaattgtggt
     7141 tacagaggta tcagtgagtc actgggctac attcaaacaa acagcaacaa atttatgggt
     7201 gaccctacgg cacgacattc tcgcgctggc cgtcttttta aatggattgc ttatttttaa
     7261 aacaatctat ggtatgtcgg tcaatttgct tgatattttc catatcaaag cattttcaga
     7321 gttggatctc tccttgctgg caaacgcccc actatttatg ctcggcgtct ttcttgtcct
     7381 gaactccatt ggcttactgt tccgggcaaa gctcgcatgg gcaatcagta tcattttgtt
     7441 gttgatagcg ctaatttaca ccctgcattt ttatccctgg ctgaaattta gtattggatt
     7501 ttgcattttt acgctggtgt ttttgctgat actgcgcaaa gacttctccc acagtagcgc
     7561 cgcagccggg acaatttttg catttattag tttcacgaca ttactgtttt actcaaccta
     7621 cggtgcgctt tatttaagcg aaggttttaa tccgcgaata gaaagtttga tgaccgcgtt
     7681 ctatttttcg atagaaacca tgtcaaccgt cggctatggc gatattgtcc ctgtttctga
     7741 atcagcacga ttgttcacta tttcggtcat tatttccggc attaccgttt ttgccacatc
     7801 tatgacctca atttttggtc cgcttatccg cgggggattc aacaaacttg taaaaggaaa
     7861 caatcataca atgcatcgta aagatcattt tattgtttgc ggacattcga ttctcgccat
     7921 caatacgatc ctgcaactga atcaacgcgg acaaaacgta acggttatca gcaacttgcc
     7981 tgaagatgat atcaagcaac ttgagcaacg cttaggcgat aacgctgatg ttatccccgg
     8041 tgacagtaat gacagttcag tattaaagaa agcgggaatc gatcgttgcc gggccattct
     8101 ggcgctgagt gataacgatg cagataacgc gtttgttgta ctctcggcaa aagatatgag
     8161 cagtgatgtc aaaacagttc tcgccgtcag tgatagcaaa aacctgaata agattaagat
     8221 ggtacatccg gatatcattc tctcaccgca actgtttggc agcgaaattc tggcgcgagt
     8281 tttaaatggt gaagagatta ataatgatat gctcgtttca atgttgttga actccggtca
     8341 tggcattttc agcgataacg atgaacaaga aacgaaagct gacagtaaag aatcagtgca
     8401 aaaatagagt ttaaagacga aagagtaaac ttattgggca ccgcaatggt gcccagtata
     8461 tttagatatt gtagatcgca aacaataacg agttacgctg tttgtttaga atgcacttcc
     8521 tgatgctgtg gatacgcata tttcggcgcg attgcccctc aagccaacgc gatttacgtc
     8581 ggctagcctg acgctgcatg cgccagcgcc ccacttccgt tctactccgc ttcatgttaa
     8641 ctactctatg caataacaga atggtcatta tacccccacc cccgacgctg accagtgctt
     8701 ttcccgcgtt tttatttgcc agatgaatcc atatgcgtaa actcataaca atgcgctttc
     8761 aaaaggattt ctaatctatg acaaccgttt atacgttggt gagttggttg gccattctgg
     8821 gatactggtt gctcattgca ggcgtaactt tacgcattct aatgaaacga cgcgcagttc
     8881 cctccgcgat ggcctggctg ttgattattt acattctgcc gttagtcgga attattgcct
     8941 atcttgccgt tggcgagctc catttaggca aacgccgcgc tgagcgcgcc agagcgatgt
     9001 ggccttccac cgcaaaatgg cttaacgacc ttaaagcctg taagcatatc ttcgccgaag
     9061 aaaatagcag tgtcgctgcg ccattattca agctttgcga gcgtcgtcag gggatcgctg
     9121 gggtcaaagg gaatcagcta caactgatga ccgagtcaga tgatgtgatg caggcgttaa
     9181 tccgcgacat ccagctcgcg cgccataata ttgagatggt gttttatatc tggcagcccg
     9241 gcggcatggc ggaccaggtg gctgaatcat taatggcggc tgcacggcgt ggtattcatt
     9301 gccgattgat gctcgactcc gccgggagtg tggctttttt ccgcagcccg tggcccgagc
     9361 taatgcgtaa tgccggtatt gaagtggtcg aagccttaaa ggtcaatctg atgcgtgtgt
     9421 ttttacgccg tatggacctg cgccaacatc gcaagatgat catgatcgat aattacatcg
     9481 cgtacaccgg cagcatgaat atggtcgatc ctcgatactt caaacaagat gcgggcgtag
     9541 ggcaatggat tgatctgatg gcgcgtatgg aaggccccat cgccaccgcg atggggatta
     9601 tttattcctg cgactgggag attgaaaccg gaaaacgtat tctgccgcca ccaccagatg
     9661 tcaatattat gccgtttgaa caggccagcg gtcacaccat tcacacaatt gcttctggcc
     9721 ccggctttcc ggaagatctc attcaccagg cattattgac tgcggcttat tcggcgcgtg
     9781 aatatttgat catgaccacg ccctactttg tgccaagcga tgatttactt catgcgattt
     9841 gcacggcggc gcagcgcggg gtggatgtca gtattatcct tccgcgaaaa aatgactcga
     9901 tgctggtcgg ctgggccagt cgcgcattct ttacggaact tcttgctgct ggggttaaaa
     9961 tttatcagtt tgaaggcggg ttactgcata ccaagagcgt gctggtcgat ggcgaactaa
    10021 gtctggttgg caccgttaac cttgatatgc gtagtctgtg gctgaatttc gagattaccc
    10081 tggcaatcga cgataaaggt tttggtgcag accttgccgc cgttcaggac gattatattt
    10141 cgcgttcacg tctgctcgat gcccgtttat ggctaaaacg tccattatgg caacgtgtcg
    10201 ccgagcgact gttttacttc ttcagtccgt tgctgtaaaa cgtcgccaac agaggttaaa
    10261 caggtagtga ctatggatat ggatctaaac aatcgcctga ctgaagatga aacgcttgag
    10321 caggcctacg atatttttct cgaactggct gccgacaatc tcgatccagc tgatgttctg
    10381 ctgttcaatc ttcagtttga agagcgcggc ggcgcggagt tattcgatcc ggcggaagac
    10441 tggcaggaac atgttgattt cgatttgaac ccagactttt ttgccgaagt ggtgattggt
    10501 ctggcggaca gtgaagatgg tgaaatcaac gatgttttcg cccgcattct gttatgccgc
    10561 gaaaaagatc acaaactttg ccatatcatc tggcgggaat aaataatcgc ttttagcgtg
    10621 aataagggct gacaactgtc agcccttatt gtgtttaaag cggatcgact ttcaggcaag
    10681 aaacggcgtg tctgaaactc ccctccagaa caggacgtgt tttggcgcac tccggaccgg
    10741 caatcgggca acgggtacgg aaaacacaac cggaaggcgg gttgatcggc gacggtaatt
    10801 ccccttccag taactggatg gttttgttct tctccagatc cggatcaggt atggggactg
    10861 ccgacatcaa tgccctggtg taaggatgta gtggattgtg gtagacctca tcataggtcc
    10921 ccagttctac cgcatggccg agatacatca ccaacacacg atcggaaatg tgttttacca
    10981 cggccaggtc atgagcgata aaaattaatg acaatcccat ctcacgttgc agctgctgga
    11041 gcaggttgac cacctgcgcc tgaattgaca cgtccagcgc cgaaaccggc tcatcgcaga
    11101 taatcagctt cggttcaaga ataagagcac gagcaatccc gatacgctgg cactgcccac
    11161 cggagaactc atgcggatag cggttaatca ggttaggcaa taacccgact ttcagcatca
    11221 tcgccttcac gcgctcgcga acttcctggc gtgacatttt cggatgataa gtacgcagtg
    11281 gttcagcgat gatctcgccg atggtcatac gcgggtttag cgatgccaac ggatcctgga
    11341 aaatcatctg aatatcactg cgaacggcac gccattcatc gggcttcatg cccagcaact
    11401 ctttacctaa ccaggcaaca tgaccgtcgg tcgccttgac caaaccgatg atggcgcgag
    11461 caaaggtgga cttaccgcat cccgattccc ctaccacacc taatgtttcc ccttcataca
    11521 ggcgaagagt gacaccatcg acggctttga gcgttttcgg cggttgccag aaccactgtt
    11581 tgccatcttt gatttcaaag tgcactttaa gatcggcgat ttcaaggagg acttttcttc
    11641 cttcagttac agcattcata acagttcctc caccggttta aagcaagcac gcagacggcc
    11701 aggcgtaaac tcttccagcg gcggtgcgct actacaaatt tccatcgcat gtggacaacg
    11761 tggctggaac gggcaacctt tcggtaatcg cagcaagttt ggcggattac ccgggatggt
    11821 caacattgtt tcaccttccg catcaagacg cggcaccgcg ttgagcaaac cgatagaata
    11881 aggatgaacg ggttgataaa agacatcgcg cgcattgcca tattccatcg tgcgcccggc
    11941 gtacattacc agcactttgt cacagatccc cgccaccacg ccaagatcgt gggtgatcat
    12001 aatgatggcg gtattaaatt cccgcttcag ttcatttaat agcgtcatga tctgcgcctg
    12061 tacggtgacg tccagcgcag tagtgggttc atccgcaatc agtagcttag gtcgacatag
    12121 caatgccatc gcaatcatga ctcgctgacg catgccgcca gaaaattcgt gcgggtacat
    12181 tttcatgcgt ttacgcgctt ccggcatttt taccgcatcg agcatccgca ccgactcttc
    12241 aaacgcctca gctttgctca tgttcttatg cagcatcagc acttccatta actgctcacc
    12301 gacgcgcata tagggattca acgaagtcat tgggtcctga aaaatcattg agatttgttc
    12361 agcgcgcagt ttattgagtt catgctctgg caaattgagg atttcacgcc cattgaaggt
    12421 tgccgatccg ccaatacgtc cgttggcagc caacaggccc atcaacgcaa atgcagtttg
    12481 cgatttaccc gaaccagact cacctacaat acccagcgtt tctccggcac gtagggaaaa
    12541 attcaaatca ttgaccgccg tgacgtcgcc gtccggggta ctaaaggtga cacgcaaatc
    12601 tttcacgttc agcagtgcgt cagcctgttg ttgtgcgagc ggcacagttg cagtttcaat
    12661 tacgctcatg gctgcactcc ttaacgatct ttcgggtcga gggcatcacg caagccatcg
    12721 ccgataaagt tgaaacaaaa cagcgtcacc acgaggaatc ccgctgggaa caacaataac
    12781 catggagaga cttccatcga gttcgcgcca tcactcagca atgcacccca gctgcttaac
    12841 ggctcttgcg tacccaaccc caggaagcta aggaaagatt caaagaggat catgctgggc
    12901 accagaagtg atgcgtagac caccaccaca ccgagtacat tcggcacaat gtggcgaata
    12961 acaatgcccg atgtcgatac accgccaact tgtgccgcct caataaactc tttgcgcttc
    13021 agactcaggg tttgcccacg cacaatacga gccatatcca gccaggaaac catgcctatc
    13081 gccacgaaaa tcagcaggat gttttgaccg aaaaaggtca ccagcaaaat gacgaagaac
    13141 atgaatggga aggagttgag gatttccagc agacgcatca ttaccgaatc cactttaccg
    13201 cccagataac cggaaagcga accataaagt gtcccgacga ccacagccac cagtgccgca
    13261 gcaacaccga ccatgagtga gatacgcccg ccaatcgcaa cgcgcacaag caggtcgcga
    13321 ccggatgagt cagtaccaaa gtagtgaccg gactccatat ccggggcgct ggacatcatc
    13381 gcccagtcag tatcgtcata ggcaaactgg gaaagcatcg gtgccaggat tacaaataac
    13441 gcgatcagca ccagcactat cagactggca accgccgcac ggttatgcat aaaacgtcga
    13501 cgtgcgtcct gccacaagct gcgcccttcg acctccagct tttcactgaa attttccagc
    13561 gtctcgctgt ttttcttact taacatcatc gcgagctcca gtatcagtaa cggattttcg
    13621 ggtcgataac cgcatatagc acatcgacaa tggcattaaa caaaatggtt aaagcaccaa
    13681 ccaggatggt caggcttaac actaaggaat agtcacggtt caatgcgcca ttaacgaaca
    13741 gttgtccaat ccccggcaaa ccataaatgg tttcgataac catagaaccg gtaataatgc
    13801 cgacaaatgc ggggcccata taagagagca cgggtaacag agcaggtttt aatgcgtgac
    13861 gtaaaatgat ccgccgcata ggtaaccctt tcgcccgggc agtacgaata aaattggagt
    13921 gtaatacttc aatcatagag ccacgggtaa tacgcgcaat actggcgata taagcgagtg
    13981 acaacgccac catcggcaat atcatgaatt taagagcccc accattccag ccaccgcccg
    14041 gcagccaatg caaaatgatc gcaaatatca tgactaataa tggcgcaacc acaaaactgg
    14101 ggataacaac cccggtcatt gccagcccca tcacggtata gtcccatttg gtgttttgtt
    14161 ttaatgcggc aataacgcca gcactaacac ccagtattac cgcaaggaaa aatgctgcgg
    14221 ctcccagttt ggcagaaacg ggaaaactgg atgcaaccag gtcattgacc gaataatctt
    14281 tatatttaaa cgatggaccg aaatcaccgt gcgccagttg tttcaggtaa ctgaaatact
    14341 gtgtcatgat cggatcatta agatgatatt tcgcttcgat attggccatc acttccggcg
    14401 gtaaagtacg ttcgccggta aaagggcttc ccggcgcgag gcgcatcata aagaacgaaa
    14461 tagtaataag aataaatagc gtcggaatcg cttccagaca gcgacgtaga ataaatttta
    14521 acattgccct accttctggc ctgtgccttc tgtaatgcga taaaaatcag acaccgtgga
    14581 gcaggacact cctgccccac gtattgccat tagtgcttca caatgtacat attccgggtg
    14641 taggtattat ccagcggatc tttgccggta tagccaccaa cccacggttt caccagacgc
    14701 gcattcacgt agtaataaac aggaacaatg gccgaatcct tatccagctg ttgttctgct
    14761 ttagtgtaca gagctgtgcg ctgcgcctcg tcagtcactt tcagcgtttc cgccataatg
    14821 ctgtcaaagg ccgggctctt ataatgcgcg gtattcatcg agctgttcga aagcatggtg
    14881 ttcaggaagg aagttggttc gttgtagtct gcacaccagc ctgcacgggc cacatcaaaa
    14941 gtgccctggt gacgggtgtc gaggaacgtt ttccactcct ggttaaccag tttgacgttt
    15001 acaccaatgt ttttcttcca caatgaagag gcagcaatcg ccagcttttt atgcagatcg
    15061 gaggtgttat acagcaggtt gatggtcaac ggtttgtctg cggtataacc cgcttcagcc
    15121 agcagttttt tcgcttcttc gttacgtttt tcctggctcc agccaaacca ttctggctga
    15181 gtcaattttg cgccatcagt atacggtgga gtgtaaccat aggcgggcat gtcgccctgc
    15241 gctttcactt tattaacaat gatatcgcgg tccataccta gtttcagtgc ggtacgcaca
    15301 cgcacatcgt tgaatggcgg tttctggttg ttaatttcgt aatagtaagt gcacaggtat
    15361 ggatcaacgt gaacttcgtc cgggatctct tttttcagct tctggaacaa ttcgatcggc
    15421 atgttgttat aagtcatgtc gatttcacca ctacggtagc ggttgacatc ggtaacttca
    15481 gaagcaatag gcaaataggt tacctggtta ataacggttt tcgcgttgtt ccagtaggtc
    15541 gggctgcgtt caagaacgat tcgttcgttt acgacccaat cttttaaggt ataggcaccg
    15601 ttggtgacga tattaccagg ctgggtccat ttttcgccga atttctcgat agcggctttt
    15661 ggcaccggtg aagttgatgg gtgaacaagt aatttataga agtacggaac gggttcactt
    15721 aaggtgactt ctaatgtgtg atcatcaata gctttcacgc cgagatcggt aatcggtttt
    15781 ttcccttcaa gaatttcatc aataccggcg atatgcccat attgcagata actggcatac
    15841 ggagaagcag tgttcggatc aacagaacgt tgccagctat acacaaagtc ttgtgctgtg
    15901 actggcgtac catcagacca tttcgcatct ttacgcaaat ggaaggtcca gactttcgcg
    15961 tctttattat cccaggattc agcgacgcca ggtgctggat gaccgtcaag atcgctgacc
    16021 agtaagcctt caaacaggtc tcggctgata ttagactccg gaacaccttc aattttgtgc
    16081 ggatctaatg actgaacttc tgaaccattg ttacgtacca gtgtttgttt ttccgccagt
    16141 gtgacgcctg cgggtacatc agctgccagc gcgacattcc ctgccattag cgcagccaga
    16201 acgccagctg ctactaaact tctcttggtg atgttggtca ttgttttttg gactccctca
    16261 ttataattac tggtattaac cagcatgtgt aatcccggat tgggaccctg cacaggctcg
    16321 gagactttct gctgtcaggt tactttatcg ttacgtatta ctatcaccga ctttatttat
    16381 tctggtgatt cagacgatca ccttatgtcg attcgtactg tgtctcccct gtcgagacac
    16441 attattcttt cgcgacgctc tttaattgtt aattattctc attaccaata aataatcgtc
    16501 tggcggttgc ccggaaagta ccaaatgcgg ttcttactcg ccaatacatt ttgcaaaatt
    16561 gttaagcaat tctcttttat tgaccgtatc agaatctata gcttgcatcc atgccttaac
    16621 aaacatcttt caattcaatc aaatagagat tggctaagca tgtctatctt ttctgattac
    16681 agttccagta gtgaaatgca caataattta
//
LOCUS       DAZFRN010000108        16622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFRN010000108 DAZFRN010000000
VERSION     DAZFRN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.110"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(146..628)
                     /gene="ybiA"
                     /locus_tag="ACHKUB_003397"
     CDS             complement(146..628)
                     /gene="ybiA"
                     /locus_tag="ACHKUB_003397"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO6656370.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            748..2898
                     /gene="dinG"
                     /locus_tag="ACHKUB_003398"
     CDS             748..2898
                     /gene="dinG"
                     /locus_tag="ACHKUB_003398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO6656371.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2926..3888
                     /gene="ybiB"
                     /locus_tag="ACHKUB_003399"
     CDS             2926..3888
                     /gene="ybiB"
                     /locus_tag="ACHKUB_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO6656372.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4029..5114
                     /gene="hcxB"
                     /locus_tag="ACHKUB_003400"
     CDS             4029..5114
                     /gene="hcxB"
                     /locus_tag="ACHKUB_003400"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO6656373.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5181..5240
                     /gene="ybiE"
                     /locus_tag="ACHKUB_003401"
     CDS             5181..5240
                     /gene="ybiE"
                     /locus_tag="ACHKUB_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO6656374.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5343..5603)
                     /gene="ybiJ"
                     /locus_tag="ACHKUB_003402"
     CDS             complement(5343..5603)
                     /gene="ybiJ"
                     /locus_tag="ACHKUB_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO6656375.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5868..6134)
                     /gene="ybiI"
                     /locus_tag="ACHKUB_003403"
     CDS             complement(5868..6134)
                     /gene="ybiI"
                     /locus_tag="ACHKUB_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO6656376.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(6208..6885)
                     /gene="ybiX"
                     /locus_tag="ACHKUB_003404"
     CDS             complement(6208..6885)
                     /gene="ybiX"
                     /locus_tag="ACHKUB_003404"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO6656377.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6927..9209)
                     /gene="fiu"
                     /locus_tag="ACHKUB_003405"
     CDS             complement(6927..9209)
                     /gene="fiu"
                     /locus_tag="ACHKUB_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO6656378.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9474..9734)
                     /gene="mcbA"
                     /locus_tag="ACHKUB_003406"
     CDS             complement(9474..9734)
                     /gene="mcbA"
                     /locus_tag="ACHKUB_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO6656379.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSSQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYIINSAGGNDQMFGTATIYK"
     gene            10010..10936
                     /gene="rlmF"
                     /locus_tag="ACHKUB_003407"
     CDS             10010..10936
                     /gene="rlmF"
                     /locus_tag="ACHKUB_003407"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO6656380.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTLAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(10933..13158)
                     /gene="ybiO"
                     /locus_tag="ACHKUB_003408"
     CDS             complement(10933..13158)
                     /gene="ybiO"
                     /locus_tag="ACHKUB_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO6656381.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPPPPGEPT
                     L"
     gene            complement(13275..13997)
                     /gene="glnQ"
                     /locus_tag="ACHKUB_003409"
     CDS             complement(13275..13997)
                     /gene="glnQ"
                     /locus_tag="ACHKUB_003409"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO6656382.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(13994..14653)
                     /gene="glnP"
                     /locus_tag="ACHKUB_003410"
     CDS             complement(13994..14653)
                     /gene="glnP"
                     /locus_tag="ACHKUB_003410"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO6656383.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(14792..15538)
                     /gene="glnH"
                     /locus_tag="ACHKUB_003411"
     CDS             complement(14792..15538)
                     /gene="glnH"
                     /locus_tag="ACHKUB_003411"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO6656384.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(15942..16445)
                     /gene="dps"
                     /locus_tag="ACHKUB_003412"
                     /gene_synonym="pexB"
     CDS             complement(15942..16445)
                     /gene="dps"
                     /locus_tag="ACHKUB_003412"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO6656385.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         3993 a         4184 c         4429 g         4016 t
ORIGIN      
        1 ctctgcaata tattgaattt gcatgctttt gtaggccgga taaggcgttt acgccgcatc
       61 cggcatttca caacaagcac ttgtcagcaa tttgagaaca cgggaaaaaa tttatctgtt
      121 ttaccgccca taagccaccg agacgttact tctctatagc caattgttcg cgcaactcca
      181 ttaaaaggta gcccagtcga ttcttgccct taccattacc accgtctccc cagtaagcat
      241 cgttttccgt atgctcaacc agttttgcgg gcgcggttgc cagcaagagc gcacgcagtt
      301 ctggatgctg ttcgaattta gcgcgaagtg cttttcgcat cacctgttct ttgaccgact
      361 cccagttttt acgcagaggc ttagaacgat cgcgccccat gcgtgcggcg accatagggg
      421 aagaaacccg acggatctct tcgcggtatt tttcatcaag gaatttttgt gcctgaaaat
      481 agtgttctga ggtgggccag gttttcccgt caaccttgat gggccaggcg gcaaagttag
      541 aaaaatcacc gtagtcatcg ctggtgctgt agaaatttat gatggtgtct tgcatgacgt
      601 gttggatcct ttgtgctcga acgggcatta aaccgcatta tgttggtggt tattgcgagc
      661 cgctttccag aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca caatattggc
      721 tgtttataca gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa agcgcaaatt
      781 gccgcctggt ataaggcgct tcaggaacag atccccgact ttattccccg tgcgccgcag
      841 cggcagatga ttgcggacgt cgccaaaacg ctggccggag aagaagggcg gcatctggcg
      901 attgaagccc ccaccggcgt tgggaaaacg ctctcctatt tgatccccgg catcgccatt
      961 gcccgcgaag agcaaaaaac gctggtggtg agtaccgcca acgtggcgtt gcaggatcag
     1021 atttacagca aagatttacc gctgctgaaa aagatcattc ccgatcttaa attcactgcc
     1081 gcttttgggc gtgggcgcta cgtttgcccg cgtaatctga cggcgctcgc cagcactgag
     1141 cccacgcaac aggatctgct ggcgtttctt gacgacgaac tgacgccaaa taatcaggaa
     1201 gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca cttataaatg ggatggtctg
     1261 cgcgatcata ctgatatcgc tatagatgac gatctctggc gtcgtttaag taccgacaaa
     1321 gccagctgcc tcaaccgcaa ctgttactac tatcgtgaat gcccgttttt tgttgctcgt
     1381 cgggagattc aggaagcgga agtggtggtg gcaaaccatg cgctggtgat ggcggcgatg
     1441 gaaagcgaag ccgtactacc tgacccgaaa aatctgctgc tggtgctgga cgaaggtcat
     1501 cacctgccag atgtggcccg ggatgcgctg gagatgagtg ccgaaatcac cgcgccctgg
     1561 tatcgactac agctggactt gttcacgaaa ctggtcgcta cctgcatgga gcagtttcgc
     1621 ccgaagacca tcccaccgct ggcgatccct gaacgtttga atgcgcattg tgaagagttg
     1681 tatgagctta tcgcctcgtt aaacaacatt ctcaatctct acatgcctgc cgggcaggag
     1741 gcagagcacc gttttgcgat gggcgaactg cctgatgaag tgctggagat ctgccagcgg
     1801 ctggcaaaac tcaccgagat gctgcgtggt ctggcggagt tatttcttaa cgatttaagt
     1861 gagaaaaccg gcagccacga tattgtgcgt ctgcatcggt tgattttgca gatgaaccgc
     1921 gcgttgggga tgttcgaggc gcaaagcaaa ctctggcggc tggcgtctct ggcgcaatct
     1981 tccggtgcgc cggtgaccaa atgggcgacg cgggaagagc gcgaagggca gctgcatctc
     2041 tggtttcact gcgtggggat acgtgtcagc gatcagctgg aaaggctgct gtggcgcagt
     2101 attccgcaca ttattgtcac ctccgcaacc ttgcgttcgc tgaacagttt ttcgcgtttg
     2161 caggagatga gcgggctgaa agagaaagcg ggcgaccgtt ttgtggcgct ggattccccc
     2221 tttaaccact gcgaacaggg caaaattgtt attccccgga tgcgcgttga gccttccatc
     2281 gacaacgaag agcagcatat tgccgaaatg gcggcctttt tccgtgagca ggtggagagc
     2341 aaaaaacatc tcggtatgtt ggtactgttt gccagcggac gggcgatgca gcgctttctc
     2401 gactatgtga cggatttacg cctgatgctg ctggtgcagg gcgatcagcc gcgttatcgc
     2461 ttagttgaac tgcaccgcaa acgcgttgcc aacggtgagc atagtgtgct ggtgggctta
     2521 cagtcatttg ccgaagggct tgatttgaaa ggtgatctgc tcagccaggt gcatatccac
     2581 aaaatcgctt ttccgcccat cgacagcccg gtggtgatca ccgaagggga atggctgaaa
     2641 agcctcaacc gctatccgtt tgaggtgcaa agcctgccga gcgcctcgtt taacctgatt
     2701 cagcaggttg ggcgactgat tcgaagccac ggttgctggg gcgaagtggt tatctacgac
     2761 aaacgcttgc tgaccaaaaa ttatggcaag cgactactgg atgcattacc ggtatttccg
     2821 atagagcaac cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac gaaatcccca
     2881 cgccgtcggc ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga ctatcgcaaa
     2941 atcattaaag agatcgggcg cgggaaaaac cacgcgcgcg atttagaccg ggatactgcg
     3001 cgcggtctgt atgcccatat gctcaatggt gaagtccctg accttgagtt gggcggcgta
     3061 ttgattgcgc tacgtatcaa aggggaaggg gaagcagaga tgctcggctt ttacgaagcc
     3121 atgcaaaatc acaccatcaa gctgacgccg cctgcaggca agccgatgcc gattgtcatc
     3181 cccagttaca acggcgcgcg taaacaggcc aacctgacgc cgttgttggc gattctcctg
     3241 cacaaactcg gttttcctgt ggtggttcac ggggttagcg aagatccaac ccgcgtgctg
     3301 actgaaacca tttttgaatt gatgggtatt acgccaacgt tgcacggcgg gcaggcgcag
     3361 gcgaagctcg acgaacatca accggtgttt atgccagtcg gcgcgttttg cccgccgctg
     3421 gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca taccctggcg
     3481 aaactggcga cgccatttgc cgaaggtgag gcgctgcgtc tttccagcgt ttcgcatccg
     3541 gaatacattg gacgcgtcgc gaagttcttt agcgatatcg gcgggcgggc gctactaatg
     3601 catggtactg aaggtgaagt gtatgctaat ccgcagcgct gcccgcagat caatctcatt
     3661 gaccgtgaag ggatgcgggt gctgtatgaa aaacaggaca ctgctggtag cgagttactg
     3721 ccacaagcaa aagatccgga aaccacggcg cagtggattg agcgttgcct tgctggcagc
     3781 gaaccgattc ccgaatcgct gaaaatccag atggcttgct gcctggtggc tacgggtgaa
     3841 gcggcaacta tcagcgacgg cctggcgcgc gttaatcagg cattttaatt cttttctcag
     3901 ccggatgagc catgtcatcc ggcttttacc ccaccgcaat atgaaattcc tgcatcttta
     3961 ttgaccttcc cacgcccggc gtgcagcata aaaaaacaac aaacacataa cataaacagg
     4021 agttaaccat ggaaagtggt catcgctttg atgctcagac gctgcacagt tttattcagg
     4081 ctgtatttcg tcagatgggt agcgaggaac aagaagcgaa attagtggcc gatcatttaa
     4141 tcgcggcaaa cctggcaggg catgattctc atggtattgg catgatccca agctatgtgc
     4201 gttcctggag tcaggggcat ctgcaaatta accatcatgc caaaatcgtt aaagaggcgg
     4261 gggcggcagt cacgctcgat ggcgatcgcg catttggtca ggtcgcggca catgaagcga
     4321 tggcgctggg gattgagaaa gcgcatcagc acggtattgc cgccgtggcg ctacataact
     4381 cgcatcatat cggccgtatc ggttactggg cggagcagtg tgcagcggcg gggtttgtct
     4441 ctatccactt tgttagcgtg gtcggtattc caatggtcgc gccgttccac ggtcgcgaca
     4501 gccgctttgg caccaatccg ttctgtgtgg ttttccctcg taaagataat ttcccgctgt
     4561 tgcttgatta cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc tggcataaag
     4621 gcgtccccgt gccgccaggt tgcctgattg acgttaacgg cgtgccgacg accaatccgg
     4681 cggtaatgca ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat aaaggctacg
     4741 cccttgcagc gatgtgtgaa attcttggcg gggcgctttc cggcggtaaa acgacgcatc
     4801 aggaaacgtt acaaaccagt cccgatgcca ttcttaactg catgaccact atcatcatca
     4861 acccggaact cttcggcgcg ccggattgta acgcgcagac cgaagccttt gccgagtggg
     4921 tgaaagcctc gccgcatgat gatgataagc cgattttgct accgggcgag tgggaagtga
     4981 acacgcgtcg cgaacggcag aagcagggga ttccactgga tgcgggaagc tggcaggcca
     5041 tttgtgatgc agcgcggcag attggtatgc cggaagagac gttgcaggct ttctgtcagc
     5101 agttagccag ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg gtaacatagg
     5161 atcaatgagg gttagagcat atgcgtctgt cggcaaacag acagggaaat acttgtgctg
     5221 gacgtagcgt aaacgcctga tccgtccaac ggttcggatt ttgtaggcct gataagacgc
     5281 ggtagcgtcg catcaggcat ctgttgccgg atgcggcgta aacgccttat ccgtcctacg
     5341 aattacttat aaattaccgc agtaccgctt aatttattgt tgttggtggc ggaagtgatg
     5401 ctgtatccac tggcaccggc tgccgcggct ttctcagcca gtttcgcttc cagggcatcg
     5461 agggtggatg cgccatcggc agaaaccacg ccgattttat tcatgttctg tgcctgggat
     5521 gccgttaccg gttccgcggc aaacacgcca aatgacagag ttgaaagagc catagcagca
     5581 acaacagtat tgatagtttt cataattaat ctctcgcagg tgatttttgt ataagtgacg
     5641 ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag agataaaatc gaagagaatt
     5701 gaccgccttg ttcaaataaa ttgattgata tctaacatac tgataaacca tttatttgtt
     5761 gtacttatgg acttgtttta tgccgcgtgg cagtcacagc agcgttaaag gcacggaatg
     5821 ccacgcaaaa tacaaattat actcgctatg catctcgcca gttgtagtta acgtaactgg
     5881 ctgtctttcg aacctctgcg attatatcct gtatacgctg gtttttgtaa atctttctcc
     5941 tgctgacaat gaatgcataa gcgcacgcca ggaatggctt cccgacgggc ttgcgggatg
     6001 ggggcaccgc actcttcaca ttcatccagg ctttcgccac gcggaatttc accccgagcg
     6061 cgggcaatcg catcttcaat tgtactgttg atctgttcgt tgacggcgtc atcgttagcc
     6121 caaccggatg ccatatcgtc ctccccatat caatgcttgt acagttaagt gtagctaatc
     6181 cagggacgaa cttgggcagt tcaagcatca gatctccgac cattcccgca gcagattatg
     6241 ataaagatta agcagcgaca ggatctcttc actttcgccg tagcggcttt tcagcgactg
     6301 aatattgttg tccagttcaa acagcatggc gcgcttttta tcatcacgga tcatcgactg
     6361 gatccacata aatgatgcca ctcgtacgcc gcgggttacg ggtgtcacgc aatgcaggct
     6421 gctggaggga tacaacacga gatcgcctgc cgggagtttt acccgatgtt gtccgaaggt
     6481 gtcattaacg accagttcgc cgccgtcgta gctttgtgga tcgcttaaaa acagcgtggc
     6541 agaaaggtca gtacgcatcc agccgttttg tggatggctg cgtactgcgc catccacatg
     6601 gaaaccatag gtttcattgt tctgatagcg attaaacagc ggcgtggaaa gggtacgcgg
     6661 caaggccgcg gcaaagaata aagcatgttg gttaaccgcg ttcagcacct cattttgcag
     6721 ggcggcgtat aacgtgctgc gggtgtcgac ctgttgattg ttcttaactt gcgcaccttg
     6781 tgcgccggtg gtgacgcgtc catccaccca ttcggcttgt tccagttgtt cgcgaaaccg
     6841 ggcgacgtcc tgtggcgata acacgccggg aatgtggtac atcatacaag ttctccaaaa
     6901 agtggggcct gcgccccaca tctgaatcag aaatgcatat tggctgtgag caagaaggtt
     6961 cttggctcgc ccgggtgata acggtagccg ctcttgttga ttgaggcgac gtaatcggta
     7021 tcaaacaggt tgtaaacgtt tagctggaag tcgagattgc gattaacccg ataccccagt
     7081 ttggcatcgg cgacccagta accttcggta aacgctggcg ttcccaccgc gccgtctgaa
     7141 cctttatgca tactgccgat atagcgtgcg cccgcgccaa cagagatatc atcggttgcc
     7201 tgatactggc tccataaggt gaaggcgtgc tccggggtat acggcagcga tgaggaacca
     7261 tcctgggcaa catctttgcc gtttttgatg gttgcttttt gctgggtata gccgccaatc
     7321 acctgccacg cgggagtgat attcccggcc acggatatct catagccttc gacacgtttc
     7381 ttaccgtatt gcgagtaagt tccgtcatca ttttgctcaa cttcattttc gatatcagta
     7441 cggaatagcg cggcggtgag caacaggcgt ttatccagaa cctgccattt ggtgccaatc
     7501 tcgctggtgt tggctttttg cggtttaaaa tcggtgcggt tggcactgtt accgctgcca
     7561 gactgcgcaa gggcgaagtt gttgccgccc ggaggctgct gggaaacggc atagttaata
     7621 tagacattac cgttttccgt caggtgatac agcgccccgg ctttccagtt caccagattg
     7681 cccgacttgg cggtgtcgac ggtggtgacc ggagaacctt ttgccacacc agttgggcag
     7741 gtgatggcac cgcgtccgct gccgccgcag gcggtggcac tgtcatattc agtatgataa
     7801 ttatccagac ggatcccgcc gttcagctca aaatcacggg tgatttgcag cgtatcgaag
     7861 gcgtaaattg cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg cgtcaggccg
     7921 ccgggatgaa tgctgctgtc aggatgataa atatttaccg cgggtaacgt caccggatta
     7981 acgccgtagt tagtctgcgt ttcacgggta aattccacgc cggtactgac atcatgaccg
     8041 atagaaccgg tatagaacgt cgaggtcagg ttggtctggt tggtgagaat tttattactc
     8101 acatctttgg tattcgccgt gcgtgaccag gtccagctat tcacatcgct ggtgggctga
     8161 gtaatattcg acgccccgcc cataatcgcc gtcatcaggt aatcctgctt tacgcgcgac
     8221 caacgggtag tattgcgaat ggtagtgtta tcgttgatgt cgtgctcaaa acgcatggtg
     8281 gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt atgagtatca
     8341 acttttccgg aatgattcag agccgctgtt cccgcagatg gggcagaata acccggcaaa
     8401 ccgatggtcg gaatgccgcc gtctggcgtg ttgtgctggg tgacatgcag ataattaaga
     8461 tacaaacgat tcgctgtacc aaggccaaaa gcgatagaag gggcgacgcc gtaacgctca
     8521 tttttgactt tgtcgcgtcc ggcatcgtgc gttttttcgc ccatcacatt caggcgcacc
     8581 gcagtggtat caccaatgac ctgattgacg tccagcgtgc cgcggcggaa ccaggcgctg
     8641 ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg gctgcttgct gatcatattg
     8701 atcgagcccg tcggcgcgct gcgcccgtag tcggtgccgg aaggcccttt aatcacttcg
     8761 acctgctcag tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat gccatcaata
     8821 taaatactgt tagaggtatc agcaccgcgc atataaatgg cgtcgccagt ggtggagtta
     8881 ccgttctcac ccgcaaaaaa cgcacccacg ccggggacgt ttttgagcgc gtcggtaagg
     8941 ttggttgcgc cctgatcttt aatcacttgt tcagaaatca ccgtcatcgt gcgggtagta
     9001 tccgctaccg gacgcgagaa tttcggatcg gcagattgtt gtggcgcata aagcgaaggc
     9061 gttgatgctt cgacaaccag cgtgtcatcc gcgttagttt gcccttcggc ggcgagtgcc
     9121 tgagccacag gcgtgatgcc aatacaaaga ccggcaaaga acgtgagcga atgaaattgt
     9181 ctggcaggga aattgcgatt gttttccatt ttgcaggtga ctttttttta tatgttgaaa
     9241 ttttcactgc cacgagaaag cctctctgcg gatgcgatgc cactgttaat ctcgtgatag
     9301 tgttcgtgca agtgataata attttgataa gcattatcac ttggcgcgat tttctatcac
     9361 ccattgcgcg aaaaagaaat acatttattt acattgcgtt catatttgat gttttttgat
     9421 tacaaaacag gcgaaaactg catccagagg cgaatgcagt tacgttggtg gggttatttg
     9481 tagatggttg cagtaccgaa catctggtca tttccgcctg cggaattaat gatataacct
     9541 ttcgcgcctt gttcgcgcgc tttttctgcc aatttatcct ccagatcgct taggtttgat
     9601 gcgccagttg ccgaaacggt accggcgggc cgtagctgac tgctgtccag gttactcatc
     9661 ggttgcgcgg cataagccgt gagagagatt ccgctcagga cggtggcaat cagtagtgtg
     9721 aggcactttt tcataatgac atcctcataa atacaacggg tagtgcctga taagtgtagg
     9781 tcgcctgggc gatgaaaatg acgcaaaaaa ttgacagaga tacgcttatt ttttgaacga
     9841 tatattttta caaaataaga caaatcaatg acatgcataa tgctgatttt tatgatgaat
     9901 tgccataaac catagccatg ttaaggtttt ggggaatgat aaacgcagcc agatatttac
     9961 cctgtcgcgt tatgcgggta tcttacgccg ctgcttaaag gagaatgcta tgtccgccca
    10021 gaaaccgggg ttgcatccgc gcaaccgtca tcacagccgc tacgatctcg ccacgctttg
    10081 tcaggtcaat cctgaactca ggcaattcct cacgcttaca ctcgccgggg agcaaagcgt
    10141 agactttgcc aatccgctgg cggtgaaggc gctcaataag gcgttgctgg cccattttta
    10201 cgccgtagcg aactgggata tccccgacgg ttttctctgc ccaccagtac cgggccgggc
    10261 ggattatatt catcaccttg ccgatttact ggctgaagca agcggaacaa ttccagcgaa
    10321 tgccagcatt ctggatatcg gcgttggtgc gaactgtatt tatccgctga ttggcgtaca
    10381 tgaatatggc tggcgtttta ccggtagtga aaccagcagc caggcgttaa gcagtgcgca
    10441 ggcgattatc agtgctaatc cggggcttaa ccgcgccatt cgtctgcgtc ggcaaaaaga
    10501 gagtggggca atttttaacg gcatcatcca taaaaacgag caatacgacg cgaccttgtg
    10561 taacccgcca ttccacgatt ccgccgctgc agcgcgggca ggtagtgagc gtaaacgccg
    10621 taacctgggg ctgaacaaag acgatgcact gaactttggc ggccagcaac aggagttgtg
    10681 gtgtgaaggt ggtgaagtcg cctttatcaa aaagatgatt gaagagagca aaggcttcgc
    10741 gaagcaggtg atgtggttta cctcgctggt atctcgtggt gaaaacttac cgccgttgta
    10801 tcgtgctctg accgacgtgg gcgcggtgaa ggtggtaaaa aaagagatgg cccaggggca
    10861 aaagcagagt cgctttattg cctggacctt tatgaacgac gagcagcgcc gccgttttgt
    10921 taatcgccaa cgttaaagcg ttggctcccc cggcggcggc ggttcagccg gggccgcgcc
    10981 cggagcaggc agcacctgat aagtctgcac tggcgcgcga acgcccgcca ggtcgaaatg
    11041 ttttttcacc tggctgtcga gggcaaagcg taccgtccac tgtttgagtg gcagcgtggt
    11101 gaacgaaaca cgcagtgtaa acgcggtatt gcttaagccg acaatcccgg caaaattcgg
    11161 ttcaccaata atcagcccgc gaatttcttc gttttccatc aattccgcta ccgcatcttt
    11221 cagtgcctga ttggctttat cagcatcttc atggcgatca acatcgtagt ttgccactac
    11281 cgaaccaata ccgcggacga agttagcaaa ggtggttatc gaagaccacg gaatgatgtg
    11341 atacgccccg gtatcctggc gcacgcccac ggagcgaatc gacatccgtt ccactgtgcc
    11401 ggtcaacggc ccgatagtta ccaaatctcc agtgttcatg ccgttttcaa actgaataaa
    11461 taccccggtg ataatatctt tcaccagcgt ttgcgaacca aacgagatag ccagacctaa
    11521 tgccccggca cctgccagca atggcgcgat attgacgccg atttccgaca acacaatcat
    11581 gatggtgatg gtactgataa tcaccgccag cgcgttacgg aacagcgtca gcagggtacg
    11641 cgtacgggcg ctgggtagcg ggcggccatg aatatccgaa gccagccggt tttcgatcaa
    11701 actggcgagc accgtccagc caaccgccga gaaaaaaaga ataagtgcga tacggatcag
    11761 gatatctacg gttttctgcc ccgcgccgtt ttgcagccag ttccagaaat cgaacaatcc
    11821 ccatgcgctc aacagcaaca ttaccgccac gcagacggtc agaatacgcg ccgttttcag
    11881 cgccgccgaa agccagccat tcagccgttt ttgcaactct ggatagttac gctgagtatg
    11941 tggcgagaga gtgatggttt ttgccagcca gcgggaaaac ataccggaaa caaacgctgc
    12001 aataccaata atcgccaggc tgcgcaccgt tgcacccatc ataaatttca ggctgttgcc
    12061 cggatcgaac aacgaaaaga aaaacagcac gataaaatag gcgcttgcca gccagtgcca
    12121 caccagcgca aaggcgcgga taaacaggct gaaaaaggcc agcgaatgct ccgcaaagtt
    12181 gagcaaatgc tgggtaatct cttttttatt acgaaagatc aggtacaacg cccagacggt
    12241 catgcacagc ataatgatga cgttcgccag cgcacctatc tgtacattca cctgattaga
    12301 gataatcggc acggccacaa tcaggccata acctatcaaa ctgcttaacc agctcaggcg
    12361 acgactccag taacgggcgc tctcgtcctg aatcgtgaac gggcgcagtt cagccacgtt
    12421 tgggcaaaaa atcaggcgta gtacggcttt gaaaaattca atcagggcaa aggcgttgag
    12481 aaacaaactt tgttggaaag cgatggtgcg actgcccgca ttcaggttgt cgcttaatac
    12541 ctggccgaca aacaatgtca gtgccagtaa cagtaggtcg ataataaacg ccccgataat
    12601 catcgccgga agctgcagcc agttgctgcg ttcgcgattt ttttgccgcg cccactggcc
    12661 cattttgcga tacagcggca gtgcgcacag acgaatcagc cagtaaaaac caaacactaa
    12721 taccgctaac attgaaaaat gggtcaacgc attgctgaag gtttgtggat taaacggctt
    12781 atgcggggag ccggtgatat tgcgataaag ttgtccgaaa cgggcggaaa gggcttcacc
    12841 ataatggcgg ctgacttcgg tgaccttttg cagcacggtt tgctcttcaa ccagcgtcgg
    12901 cggcacgatc ttcgggaccg gttccgcagg gggcgtcgcg gcgacagtac gcaactggtc
    12961 gatcaattct ttacgcgagg tgtcgttatc cagcacatcc gccagtgcgc cataggccgc
    13021 ttttttttgt tcgatatccg gttccggggc cggttcagtc gttgagtccg ttgtcgttgt
    13081 ggttgtaacg ccgggtatgg ataccgcgtg ggcaggtgcg cccagcaggc agaagaggat
    13141 gaacaggatc caccgcatga ctcctccagt gagaaaacag agcaaaaaga taagtataga
    13201 tgctggggga ggggcgattt taaatgagag gaatctggtg tgcctccctt tcgggtgaaa
    13261 gggaggcaga gaagttaaga gacgtgctgc aaaaattcct gcaagcgctg gctcggcggg
    13321 ttcttgatca atacctgcgg atcgccatct tccgcaatcc ggcctttgtc gataaagatc
    13381 agccgcgaag ctactttctc ggcaaaaccg atttcgtggg tcacgatcac catcgtcatc
    13441 ccttcttcag ccagatcctg cataaccttc agcacttcat ggcgcagttc cgggtcaaga
    13501 gcggaagtcg gttcatcaaa cagcatcatt ttcggcttca ccgccagcgc gcgggcaatc
    13561 gccacacgct gctgttgacc accagaaagt tcggaagggt agtgatgtgc acgttctgcc
    13621 agaccgactt tcgccagcag ctcacgcgcc agtttttccg cctcttcttt gttcgcgcca
    13681 cgcacgcgta gcggaccaaa catgacgttt tccagcgccg tcagatgcgg gaagaggtaa
    13741 aactgctgga acaccatacc cgcttcctgg cgaatcaggc gctcgtcaac tttcggatcg
    13801 ttaaccttca ggccatcgac aatcagatcg ccggaggtga tttcttccag tttgttgatg
    13861 cagcgcagca gggtcgattt accggaaccg gacggcccga taatcaccac gacttcgccc
    13921 tgagcaatgt tcaaatcgat attgtgcagc acctgggttg gaccaaaatg cttggagacg
    13981 tttttaaatt caatcacagg attttcatcc ttctttccag acgacgcaga ataaagctca
    14041 gcaccagggt aataatcaga tagaacaccg ccacggcgct ccagatctca agggcgcgga
    14101 agttaccggc aataatttct tgcccctgac gggtcagttc cgccacgccg ataacaataa
    14161 acagcgaggt gtctttaatg ctgatgatcc actggttacc cagcggcggc agcatacgac
    14221 gcagtgccag cggtaaaatg acgtagcgaa tggtttccca acgtgaaaga ccgagcgcca
    14281 gtcctgcttc acgaaaacct ttgtgaatag acagcaccgc accacgcgtg atttccgcaa
    14341 tgtacgcgcc ggagttgatc atgatggtga ccaccgccgc agtaaatggg tcgatgcgta
    14401 agtcgttaaa cgccatcggc agggcgaaat agataaacat cacctggacg acgataggtg
    14461 tgccgcggat cacttcaata aagaccagcg cgacgtggtt ggctatccaa ccaccgaagg
    14521 tgcgtgcaaa acctgccagc aatccgatta ccagaccgcc tgccagaccg aggaccgaaa
    14581 tccacagggt cattttggca ccttcaatca gaagcggaat ggcaggccag atggcactcc
    14641 agtcaaactg catatgttgt tcctgttacc gtggtgtaaa taatcaaatt cagggtaatt
    14701 aatggccccg gagcgggttt gcgcccctca ccctaatcct ctccccatag gggagaggga
    14761 actgccagtg cgttttacag gtgtagcgtt attatttcgg ttcagtaccg aaccattttt
    14821 tgtagatttc gttgtaagtt ccgttctcgc gcagggtttt caacgcgccg ttgactttgt
    14881 cacgcagctc gtcgctacct ttcgggaacg caataccgta ttgctgcgct tccagagagt
    14941 cacctaccgc tttgaactga ccgttaccgg cggttttgat gaagtacaga atgtttggcg
    15001 tatcgtgcag aacggcgtct gcgcggttgg tgcccagttc catataggcg ttatcgatgt
    15061 tcgggaactg acgcagatct ttagttttga tgtttgcttt cgcgtaatca acggagccag
    15121 taccgctctt cacagcaacc actttcccgt cgagatcttt cacgcttttc acatcgttat
    15181 tgttagcttt caccatcact aacaggccgc ttttgtagta gccgtcagag aaatcgatcg
    15241 cttttttacg ctcgtcggtg atggtaatgc ccgccagcgc cagatcgacg tttttggttt
    15301 gcagtgccgg aatgatccca ctgaaatcca tcggcttcag ttcgtaatcc agcttcagct
    15361 ctttagcgat ggcagcccac agatcaacgt caaagcccac atatttatcg ccctgtttaa
    15421 attcaaacgg aacgaaggcg gtatccgtcg cgacaactaa ttttttatcc gcggcatgag
    15481 aagaaaccgc aaaagccagg gtcagtgcag ccagtgaaac ttttaataca gacttcatag
    15541 catttccttt tttctatcca cgggacgatc ccctgcgtga cattcacata tatgaaaaaa
    15601 tcgtgccagt tttgcaactc cttgattttt cgagatgcgg caaaaacgtg atgcacgatt
    15661 tatagggcaa taccctgaag atgcaccatt ctggggcacc aatctggtgc gctaaaattg
    15721 tgcactcaac acagtgatta tttagcgtaa aacctgatga aaaaacaatc tttatgtaac
    15781 gattgtgtga tgatgtggat acaaaaaatt tacttaatca gctggagata gcagatggat
    15841 gcactaaata agtgcgttga ggtgggctgc aaaacaaaac ggcctcctgt caggaagccg
    15901 cttttatcgg gtactaaagt tctgcaccat cagcgatgga tttattcgat gttagactcg
    15961 ataaaccaca ggaatttatc caggtcgcga gacgcggcgg tcaggatatc tgcggtgtcg
    16021 tcatctttcg cttcgccaat cgctttgcgt acgtcattag cgacgattgc gtaacggtca
    16081 gccagttctt tcaggtgatc ctgaacgttg tggatgtcca gcgggtaact tttcagcggg
    16141 gttttgctgt tgataacttg agtggtcccc agagctacac cgcccagctg cactgcacgt
    16201 tctgccatgg tatccagatg atcgatcagt gcggtgcgga agccatccag catttcatgt
    16261 acggcaatga agttagcgcc gcgcatgttc cagtgcgctt gtttggtaat caaagaaaga
    16321 tcaataaact ggataacctg gcgattcagc aactctactg ttgctttttt ctcgctgtcg
    16381 gagacatcgt tgcgggtata aagcagattg gtcgcttttg atttaactaa tttagcggta
    16441 ctcataattt catatcctct tgatgttatg tcccagtaat taacgagact aagtatagca
    16501 ccggctatgt gttccgctat tctggctgtt cctatcacac taatagtggt aacaagcgtg
    16561 aaaaacaaaa ctaataaaga ttcaatgagt tagatatatt gataagaaca attcttattt
    16621 at
//
LOCUS       DAZFRN010000109        16573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFRN010000109 DAZFRN010000000
VERSION     DAZFRN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.111"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(150..956)
                     /gene="trpA"
                     /locus_tag="ACHKUB_003413"
     CDS             complement(150..956)
                     /gene="trpA"
                     /locus_tag="ACHKUB_003413"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO6656386.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(956..2149)
                     /gene="trpB"
                     /locus_tag="ACHKUB_003414"
     CDS             complement(956..2149)
                     /gene="trpB"
                     /locus_tag="ACHKUB_003414"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO6656387.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2161..3522)
                     /gene="trpCF"
                     /locus_tag="ACHKUB_003415"
     CDS             complement(2161..3522)
                     /gene="trpCF"
                     /locus_tag="ACHKUB_003415"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO6656388.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="ACHKUB_003416"
     CDS             complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="ACHKUB_003416"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO6656389.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="ACHKUB_003417"
     CDS             complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="ACHKUB_003417"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO6656390.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6702..6795)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="ACHKUB_003418"
     CDS             complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="ACHKUB_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO6656391.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6954..7835
                     /gene="rnm"
                     /locus_tag="ACHKUB_003419"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6954..7835
                     /gene="rnm"
                     /locus_tag="ACHKUB_003419"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO6656392.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7832..8452
                     /gene="yciO"
                     /locus_tag="ACHKUB_003420"
     CDS             7832..8452
                     /gene="yciO"
                     /locus_tag="ACHKUB_003420"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO6656393.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8553..9425
                     /gene="rluB"
                     /locus_tag="ACHKUB_003421"
     CDS             8553..9425
                     /gene="rluB"
                     /locus_tag="ACHKUB_003421"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO6656394.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9465..10055)
                     /gene="cobO"
                     /locus_tag="ACHKUB_003422"
     CDS             complement(9465..10055)
                     /gene="cobO"
                     /locus_tag="ACHKUB_003422"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO6656395.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10052..10810)
                     /gene="yciK"
                     /locus_tag="ACHKUB_003423"
     CDS             complement(10052..10810)
                     /gene="yciK"
                     /locus_tag="ACHKUB_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO6656396.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11030..12079
                     /gene="sohB"
                     /locus_tag="ACHKUB_003424"
     CDS             11030..12079
                     /gene="sohB"
                     /locus_tag="ACHKUB_003424"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO6656397.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12115..12366)
                     /gene="yciN"
                     /locus_tag="ACHKUB_003425"
     CDS             complement(12115..12366)
                     /gene="yciN"
                     /locus_tag="ACHKUB_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO6656398.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12746..15343
                     /gene="topA"
                     /locus_tag="ACHKUB_003426"
     CDS             12746..15343
                     /gene="topA"
                     /locus_tag="ACHKUB_003426"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO6656399.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15553..16527
                     /gene="cysB"
                     /locus_tag="ACHKUB_003427"
     CDS             15553..16527
                     /gene="cysB"
                     /locus_tag="ACHKUB_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO6656400.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4048 a         4193 c         4410 g         3922 t
ORIGIN      
        1 atattactgt tgggcggaaa aatgacgtaa gttgacgttc gaccggggta agcgaaacgg
       61 taaaaagata aatattaaat gaatttagga tttttccggc ttcattaaag aaagttaaaa
      121 tgccgccagc ggaactggcg gctgtgggat taactgcgcg tcgccgcttt catcggttgt
      181 acaaaaactt tcagtgccgc cagcattttc tctggctcat taatatgttg ctcgatgatt
      241 ttaacaatgg ccgaaccaga aatcgcgccc gcagctcctg catcaatcgc tgcttttacc
      301 tgatccgggg cggaaatacc aaacccctgc aatggaggtg cagcgttgta ctctttcagc
      361 ttcgcaacca gatgattgag gggtaacgcg gcgcggtttt ctgcgccggt cacgcccgct
      421 cgtgacagca aataggtgta accacgaccg taagaggcta tctggcgcag caggtcgtca
      481 tcggcatttg gcgggcagat gaagataggt gcgacattat ggcgcaacgc ggcctggcgg
      541 aagggcgcgg actcttcaat tggcacatcg gcaaccagca ccgaatcgac gccgactttc
      601 tcgcactggg cataaaactc atcaatgcct ttgttaaaca ccagattggc atacatcaac
      661 aggccaatgg gaatggtcgg gtgtttctgg cgaatcagtg ccagcatttc aaaacattgt
      721 gccggagtca cacctgccgc aaaggcgcgc agagtggcgt tttgaatcgt cgggccatcc
      781 gccagtgggt cggagaaggg gatacctaac tccagcgcgt cagcaccggc ttcaattagc
      841 gtatcgataa ttttcaatga ctgctcaatg cccggatcac cgagcgtgac gaaaggaacg
      901 aatgcgcctt ctttgcgctc cttcaactgg gcaaacagag attcgtagcg ttccatcaga
      961 tttcccctcg tgctttcaaa atatcgtgaa cggtgaagat gtctttatcg ccgcgaccgg
     1021 aaaggttaac caccagtagc tgctctttat ccgggttttc gcgcatcatt ttcaacgcat
     1081 gggccagggc gtgggaggat tccagcgccg ggatgatccc ttcgtgcagg cacagcgttt
     1141 tgaaggcttc aagggcttca tcatcggtaa tagacacgta atcagcgcgt ccagtgctgt
     1201 taagatacgc gtgttgtggg ccgacagacg ggaaatccag tccggcggag atggagtaag
     1261 attcttcaat ctgcccgtct tcggtttgca tcatcggcgc tttcataccg aaatagatgc
     1321 ccacgcgacc atgttttaac ggtgcgccgt gctcgccagt ttcgataccg tgaccacctg
     1381 gctccacacc aatcaggccg acgttggttt cattgatgaa atcagcaaac atgccgatgg
     1441 cattcgaacc gccgccaaca caggcgataa cggcatccgg caggcgacct tctctttcca
     1501 gaatctgcgc tttggtttct tcgccaatca tccgctgaaa ctcacgcaca atggtcggat
     1561 aaggatgcgg gccagctgcg gtgcccagca tatagtgcgc ggtttcgtaa ctaccggacc
     1621 agtcgcgcag cgcctcgtta caggcatctt tcagcgtcgc ggaaccgcta tgcaccggga
     1681 tcacttccgc acccattaag cgcatacgaa aaacgttagg cgactggcgt tctacgtctt
     1741 tggcacccat ataaatacgg catttcaggc cgagcagggc gctggcaagg gccgacgcca
     1801 cgccatgctg accggcaccg gtttcggcga tgatttcggt tttacccatc cgcttcgcca
     1861 gtaaagcctg accgagcacc tggttagttt tatgcgcgcc gccgtgcagc aaatcttcac
     1921 gcttgagata cagagtggtg ttcgtcccgg ctgtaatgtt ctggcatttg gtcagcgcgg
     1981 ttggacgccc ggcatagttt ttcagcaggt cgttgaactg agcctgaaat tcaggatctt
     2041 tttgcgcact gacaaaagct tcttccagct ggcgcagagc aggcatcagg atttgtggca
     2101 cgtacatgcc gccaaactca ccaaaatagg ggttaagtaa tgttgtcatt gttcctttcc
     2161 ttaatatgcg cgcagcgtct ggaaaaccga ggccaaaaga cgtgcgtctt tgatgcccgg
     2221 ttgcgactct acagcagaat taaaatcaag tccggcgcag ccggtttgtg ccgcttccac
     2281 gcagttatct gcgcctaagc cccccgccag cagaacgttg ccaagcgatt gaccatttaa
     2341 tagtgaccag tcgaaacgtt gcccgcttcc accctggccg ttgtctaaaa catatttatc
     2401 aacgtgctga agctcgcggg cgggcagggt ttcaccgacg cttaatgctt tccagatggc
     2461 aacgtgtgct ggcagagctt cacgcagcgt atcgatatac agctgatctt cattaccatg
     2521 cagttgcact gccgccagcg ataacacctt agctttgtcc accacatcgg caatatcgtg
     2581 attgcggaac acgccaacat actgcaacgg tgccgcagcc atcacttcct gcgcctgttc
     2641 aacgttgacg caacgcggtg atgtcgcaac aaaaatcaac ccaccgtaaa tcgcgcccgc
     2701 gtcataagct gctttagcat cttgcccacg cgtcaggcca catactttat tctcacccag
     2761 caacacccgg cgcacggcgg cgtgcaaatc gtcatgggcc atcaacgccg aaccaatcag
     2821 aaaaccgtta gcgaagtggc ttaactcgcg cacctgagcg taagtattga tgccggattc
     2881 gctgattacc gttacgttgt gccccagttt cggcgcaagc tcgcgggtac ggttgagatc
     2941 aatcgacaaa tcccgcagat cgcggttgtt gatgccaacg acctttgccc ccaatgcaat
     3001 ggcacgctcc agttcctctt cattactgac ttcggtcagc acacccatct ccagactgtg
     3061 ggcgacggcg gcaagctggc gatattgttc gtcatccagt actgaaagca ttaataagca
     3121 ggcatcagcc tggtaatagc gcgccagata gatctggtaa ggatcgataa tgaagtcttt
     3181 acataaaatc ggctgcgggg cgatttggct gacgatgggg aggaaatcaa agctcccctg
     3241 aaaatatttc tcatcagtca gcaccgaaat tgccgaagcg taatgtttat aaatggcggc
     3301 aatgcgtgct ggatcgaaat catcacggat cacgcctttt gacggcgacg ctttcttgca
     3361 ctccagaata aacgccgtgc gcgcaccctg tagcgcatca taaaaatgtc gcgtgctcgg
     3421 ctgaacctca ttctgaaaac tggccagcgg ttgctgctgt ttgcgggttt ctacccaaat
     3481 cgccttgtct gcgacgattt tcgctaaaac ggtttgcatc atttaccctc gtgccgccag
     3541 cgcggtaact ctgtcgtaag cggaaccact gcgcagtacc tcaagaacgg tttgcgcatt
     3601 ggcttgcaga tcttcatggc catgcaggcg cattaacatg gcgacgttcg cagcgacggc
     3661 tgcttcatgg gcggcgtcgc ctttaccttg taacaaacgt gttaaaatgt cacggttttc
     3721 ttccggtgtt ccgcctgcca gttgctcctg gtggtagggt gtcaggccaa aatcttcagc
     3781 ggtcaattga tagctcttaa tttcgccgtc atgcagctcg gcaacgattg tcggcgcgtg
     3841 taatgaaact tcatccatcc cgccgctgtg caccaccgcc gcgcgttgat accccagcac
     3901 gcgcaaggtt tcggcaatcg gcagcaccag ttccggacta taaacaccaa ttaacgccag
     3961 cggcggatgc gccgggttaa tcaatggccc cagcacattg aacagggtgc gggttttcag
     4021 ttgctggcga accggcatcg cgtggcggaa tccggtgtga tacttcggcg caaagaggaa
     4081 acatacacct aactcatcca gcgcctggcg cgatttatcg gcgttcatat caagattaat
     4141 accgaacgcc gccagcagat cggacgaacc agatttactg gagacgctac ggttgccgtg
     4201 tttcgccact ttcagcccac aggccgcggc gacaaacgca ctggcggtag aaatattgat
     4261 actgttgctg ccgtcaccgc cagtaccgac gatatcagca aacagataat ccgggcgcgg
     4321 gaacggcgcg gcgttttcca gtagcgcggt tgctgctccg gcgatctcgt tcgggtgctc
     4381 accgcgaatt ttcatgctca ccagcgccgc cgccagttgt tccggcttca gctcgccacg
     4441 caccaccgct gaaaacagct ggtggctttc ttgttggcta agcgtctgcg cctgatacag
     4501 tttttccaga atcggttgca gcgtgttggc tggctctagt ttctgctgcg cccaggccag
     4561 cgtttgttcc agcaggcgag cgccctgggt ggtgagaatg gattccggat ggaactggaa
     4621 tccacaaacg cgatccgcat cgtgacgtac tgccatcacc atgccattaa aatgggcgtt
     4681 gatggttaaa ccggccggaa tgttactgcc aaccagcgag tgataacgcg ccaccggcag
     4741 cgggtttgtt aatccggcaa acatcgcctg accgtcatgt tcaatgctcg acgctttacc
     4801 gtgaagaatt tcgcccgcct gaccgacata gcccccgtaa gcttcgacaa ttgcctgatg
     4861 tccgaggcaa atgccaataa tgggcagctt gccacgcaag cgggtgagga gttccggcat
     4921 acaaccggct tcgctcggca caccggggcc aggagaaagc atcagtaccg gattgctcat
     4981 cgtcgccagg cgttcaatta aggtctgcgc cggaatatgg ttgcggtaaa tcaccacatt
     5041 atgcccattg ctgcgcaact gatctgccag gttgtacgta aaagagtcga tattatcgag
     5101 cagcagaatg tcagccatca gaaagtctcc tgtgcatgat gcgcggtggc aatagcgcgc
     5161 agtacagcgc gggctttatt acgggtttcg tcggcttccg actgcggaac agaatcaagg
     5221 actacgccag caccggcttg cacggtggcg ataccgtttt ccaccagcgc cgagcggatc
     5281 acaatgcagg tgtcgagatc gccatgcgcg gtgaaataac ctaccgcgcc gccgtagctg
     5341 ccgcggcggc gaccttccgc ctcggcaatt aactgcatag cgcgtacttt cggcgcacca
     5401 cttaacgttc ccatattcat acaggcgcgg taagcgtgca gggcgtcgag atcgtggcgc
     5461 agttcaccga ccacgcggga gaccaggtgc atcacgtaag agtaacggtc aactttggtg
     5521 aggtcggcga cgtagcggct gccgggggtg caaatgcgtg ccagatcatt acgggcgaga
     5581 tccaccagca tcagatgttc agaaagctct ttatgatcgg tacgcatttc cagttcgatg
     5641 cggctgtcga ggtctctgtc cagcgagcca tcggcacgac gaccgcgtgg acgtgttccg
     5701 gcaatcgggt agatctcaat ctggcggctg gtggcgtcat acttgagcga actttccggc
     5761 gacgcgccaa acagggtgaa atcattatcc tgcataaaaa acatgtacgg gctgggatta
     5821 ctctttttca gcacgtaata ggccgccagc ggtgacgggc agggcagaga gaaacggcga
     5881 gatggcacca cctggaaaat ttctccagcg cgaatcgctt tttgcaacaa acgcactacg
     5941 ccaccgaact cttcatcgct ctgattacat tcacaacgca tatgcggcac ggaaaccact
     6001 ggcagcggcg gcgcggcttc ggtcagttgc tgacgtagtt cgttcaggcg agcagtgaga
     6061 cgttgttttt cttcttcatt cggagcaaac aggctggcct gaatgcgggt gctttttttc
     6121 tgatggtcaa tcaccatcag agtttcagcg agataaaaac agaaatcagg gcagttattt
     6181 tccgctgaca gttgcggtaa atcttcaaat cccgccacaa ggtcataaga gaacaggccg
     6241 ccgaagaaca ttgcttctcg ttcttccttc ggtacattca acagattctg caataaacgg
     6301 aaagcgtcaa aaaccgaaag ggagcataag cgagcgtctt catccagcag tggactgaca
     6361 ggggggaagc gcagcacacg gcagtttggt aattgttcat tttccacacc cgcaggcagg
     6421 gcgttatcca gtagtgtcag cagggcttcg ccgttgccgg aaagtgcctg gattgtgaca
     6481 gtgtcaccta aagctgtaat gcgcagcgca ctgtctacca gcagcaggct ttttaaatca
     6541 tctttgctgt caatatctgc ggattccagc agcagcgttg ccggacgatc cccacacaac
     6601 tggtgaaaaa gcgcggtggg attgtcgcga taagcgcctt cgcaggttag cagttcgaga
     6661 gtcggttttt gtgtttgcat tgttattctc taattttgtt caaaaaaaag cccgctcatt
     6721 aggcgggctg ggtatctgat tgctttacgc atggtgaata cactgcccgt ttcaggaagt
     6781 gcgccaccaa cctttcagta cgaaaattgc tttcattgtc gatacccttt ttacgtgaac
     6841 ttgcgtacta gttaactagt tcgatgatta attgtcaaca gctcatttca gaatatttgc
     6901 cagaaccgtt atgatgtcgg cgcaaaaaac attatccaga acgggagtgc gccttgagcg
     6961 acacgaatta tgcagtgatt tacgacctgc acagccatac cacagcttcc gatggctgcc
     7021 tgacgccaga agcattggtg caccgtgcag tcgagatgcg cgtcggcacc ctggcgatca
     7081 ccgaccatga caccacagct gcaatcgcgc cagcaagaga ggaaatttca cgttcgggac
     7141 tggcgttgaa tcttattccc ggcgtggaaa tttccacggt ctgggaaaat catgaaattc
     7201 atattgtcgg gctgaatatt gatattactc atccgctaat gtgtgagttc ctcgcgcagc
     7261 agacagaacg gcgcaatcag cgggcgcagc tgattgccga aagacttgaa aaagcgcaaa
     7321 tacctggggc gctggaaggc gcacaacgac tggcgcaggg cggcgcagtg acgcgcggtc
     7381 attttgcgcg tttccttgtt gagtgcggca aagccagttc aatggcggat gtctttaaaa
     7441 agtatctggc gcgcgggaaa accggatacg ttccgccaca gtggtgtaca atagaacaag
     7501 ctattgatgt cattcatcat tctggcggta aggcggtgct agctcatcct gggcggtaca
     7561 atcttagtgc taaatggctg aaaagattgg ttgcgcattt tgccgaacac cacggtgacg
     7621 cgatggaagt cgcgcagtgc cagcaatcgc ccaatgaacg tacccagctg gcggcccttg
     7681 cgcgtcagca tcatttatgg gcatcacaag gatctgattt tcatcagcca tgcccgtgga
     7741 tcgaactggg tcgtaaactc tggctgcccg caggcgttga aggcgtctgg cagctatggg
     7801 aacagccgca gaacaccaca gagagggaat tatgagccag tttttttata ttcatcctga
     7861 taacccacag caacgtctga tcaaccaggc ggtggagatc gtgcgtaaag gcggggtgat
     7921 tgtttatcca actgattccg gctatgcgct cggctgtaaa attgaagata aaaacgcgat
     7981 ggagcgtatt tgtcgtattc gccagctgcc ggacggtcac aactttaccc tgatgtgtcg
     8041 cgatctttct gaactctcga cctattcatt tgttgataac gttgcgtttc gtttaatgaa
     8101 aaacaacacg ccgggcaact ataccttcat cttgaagggg acgaaggaag tgccacgccg
     8161 tctgttgcag gaaaaacgca aaaccatcgg tatgcgtgtg ccttctaacc cgattgcgca
     8221 ggcgttgctt gaagcactgg gcgaaccgat gctttccact tcgctaatgc tgccaggcag
     8281 cgaatttacc gaatccgatc ccgaagaaat taaagaccgt ctggaaaaac aggtggattt
     8341 gattatccac ggcggctatc tcggacagaa accgacaacg gttattgatc ttaccgatga
     8401 tacgccggta gtggtgcgtg aaggcgtagg tgatgtgaag cctttcttat aagtgcgtaa
     8461 ctctgtatac tacgcggcct taaaatggcg ctatacggcg ccattgattc tcctgttcga
     8521 cgcctgtgaa ggcgacacca aaaggaagct ctatgagcga aaaattacag aaagtgctgg
     8581 cccgcgctgg ccacggctcc cgccgtgaaa tcgaatctat tattgaagcc ggtcgtgtga
     8641 gtgttgatgg caaaattgct aaactcggcg atcgcgttga agttacccct gggctgaaaa
     8701 ttcgtatcga tggtcatctg atttcggtac gtgagtccgc tgaacaaatt tgtcgcgttc
     8761 tggcctatta caaaccggaa ggtgagttgt gtacccgtaa cgatccggaa gggcgtccga
     8821 cggtgtttga ccgtctgcca aagctgcgtg gcgcacgctg gattgccgta ggtcgtctgg
     8881 acgttaacac ctgtggtctg ctgctgttca ccaccgatgg tgaactggca aaccgtttaa
     8941 tgcatccaag ccgtgaagtt gaacgtgaat acgccgtgcg tgtatttggt caggttgacg
     9001 acgcgaaact gcgtgatttg agtcgtggcg tgcaactgga agatggtccg gcagctttta
     9061 aaaccatcaa gttcagcggc ggcgaaggga tcaaccagtg gtacaacgtg actctgaccg
     9121 aaggccgtaa ccgtgaagtt cgtcgtctgt gggaagcggt tggtgtgcag gtgagccgcc
     9181 tgatccgtgt tcgttacggt gatatcccac tgccaaaagg tctgccacgc ggtggctgga
     9241 cggagctgga cctcgcccag actaactatc tgcgcgaact ggtggagcta ccgccagaaa
     9301 ccagctctaa agtcgctgta gaaaaagacc gtcgtcgcat gaaggctaat cagattcgtc
     9361 gtgcggtgaa acgtcacagt caggtgagcg gcggtcgtcg ttctggcggg cgtaataacg
     9421 gttaatcaaa gtatgccgga cgtcatatcc ggcattttta cagattaata atcaatccct
     9481 atttgcgcct taacaccggc atcaaacgca tgtttgatgg ggcgcagctc acttaccgta
     9541 tccgccagtt caagaatatc ccgatgacag cctcgacccg tgataatcac cgtctgttga
     9601 tgtggacgtt catttaacgc ctgcaccact tcttccagtg gcaaatagtc atacgccacc
     9661 atatacgtca gttcatcaag caaaaccata tccagtgagg aatcagcaag catccgcttt
     9721 gcatgttgcc agacttcgcg gcaggcggcg gtatcagact cgcggttttg tgtatcccag
     9781 gtaaagcccg ttgccatcac ctgaaactca acgccatgtg gctccagcag attgcgttcg
     9841 ccattaggcc aggtgccttt aataaactgc acgacgccca ctttttttcc gtgaccaact
     9901 gcgcgtgttg ccgtaccaaa tgccgcggtg gttttgcctt ttccattgcc ggtaaagacg
     9961 atgataatac cgcgttcatc ctgggcctgg gccacacgag catctacttt ttctttcact
    10021 cgctgctgac gctgttggta gcgttcatca ctcattggga aattcctggt ttacggcccg
    10081 gttgggcgtc aaaggtcatg ccggttttac ggcggctgtc atcgcccatc agccagaggt
    10141 agagcggcat gatatcagcg ggtgttttaa gtttctgtgg atcttcggtc gggaaggcgc
    10201 ttgcgcgcat tgcggtgcgc gtaccgcctg ggttaatgca gttgacgcgc aggcgctgct
    10261 gatattcatc ggccagtacc tgcatcatcc cttcggtggc aaatttcgac gctgcatatg
    10321 caccccagtt agctcgtccc tgacgtccaa cgcttgatga agtaaagacc agtgaaccgg
    10381 cgtccgattt gagtaataaa ggaagcagtg cctgggtgag cataaaggtg gcattaacgt
    10441 tgacctgcat gacgtcctgc cagacctgcg gattttgttc gctcattggg caaatatcgc
    10501 cgagcaatcc ggcattatgc aaaacaccat ccagacgcgg ataattaacg acaatgcgct
    10561 gtgccagttg ttggcaatct tcggacgtgc aggtcagcaa atcgagaata aaccactgtg
    10621 gctgacgtcc agtttcttcg tttatgtggc tggctacctg acgtaatttt tcttcattac
    10681 ggcccaacag aatcactgtc gcaccatagc gtgcatacgt catcgcggct tcacggccaa
    10741 tcccatcgct ggctcccgtc accaggataa tgcgatcatt gagtaaatct tgttttggct
    10801 ggtaatgcat ggctactcct caacgacgtt gtctgtcgcg ctgccagtgt actttatgac
    10861 tttggggctt tatgcctgaa acagaagggc atttcaatca gcctggggcg ataacaccgc
    10921 aaaatggata ctttgtcata ctttcgctgc aataacatct ctgcgagacg gcttaacatg
    10981 cctgttgtaa actgtgagcc aaagcgttgt ttaaccaagg tggggactcg tggaattgtt
    11041 gtctgaatat ggtttgtttt tggcgaaaat cgttaccgtt gtgctagcga ttgcggcgat
    11101 tgccgccatt attgtcaatg ttgctcaacg taataaacgc cagcgtggcg agttacgggt
    11161 caacaatctc agcgaacagt ataaggagat gaaagaagaa ctagccgcgg cgctgatgga
    11221 ctcacatcag caaaaacagt ggcacaaagc gcagaagaaa aagcacaagc aagaagcgaa
    11281 agcagcaaaa gcgaaagcca aactgggcga ggtggcaact gacagtaaac ctcgcgtctg
    11341 ggtgctggat tttaaaggca gcatggacgc ccatgaagtg aactcgctac gtgaagagat
    11401 aacggctgta ctcgcagcat tcaaaccgca ggatcaggtt gtgctacgtc tggaaagccc
    11461 tggtggcatg gtgcatggtt acgggttggc ggcttcgcag ctgcagcgtc tgcgtgataa
    11521 aaacattcct ttaactgtta cggtagacaa agtcgctgcc agcggcggtt acatgatggc
    11581 ctgtgtggcg gacaaaattg tttccgcacc gtttgctatt gtgggttcca ttggggtggt
    11641 ggcgcaaatg cccaacttta accgcttcct gaaaagcaaa gatattgata tcgaactgca
    11701 cactgccggg cagtataagc gtactctgac gttgctgggt gaaaataccg aagaagggag
    11761 ggagaaattc cgcgaagagt tgaacgaaac gcatcagttg tttaaagatt ttgtgaagcg
    11821 tatgcgtccg tctctggata ttgaacaggt ggcaacgggt gaacactggt acggacaaca
    11881 ggcggtagag aaaggcctgg ttgatgaaat caacaccagt gatgaagtta ttcttagcct
    11941 gatggaaggc cgtgaagtgg ttaatgtacg ctatatgcag cgtaaacgac tcattgaccg
    12001 attcaccggc agcgcggcag agagcgccga tcgattgttg ctacgctggt ggcagcgggg
    12061 tcaaaagcca ttgatgtaaa agacaaacgc gaggctaaga cctcgcgttt tgctttaatc
    12121 aaccagatga tatttttctg aaagcacatg ggccaggtgt ttgaacatat taaacaccgc
    12181 ggtgcttttg gctgttggca atccttgttc atctaaaaag tagtcgccgg taaataccag
    12241 cacgccatta cgctgcgtga cgccggtggc ttcaatccct gcgagcgtat cctcatgctc
    12301 acgaatgatt ttgttggcct ctttcaacag cgtttcgcgg tcgatgggtt gtgtctcttt
    12361 gttcattatt tactccttaa acaaggacat tagtctacgc caggcatggc ttgcagacaa
    12421 atataccacg ctggtggcaa gagcgcctta ctggtaactt tggattttgc atgctaataa
    12481 agttgcgtat cggattttat caggtacagt gtgacgcttt cgtcaatctg gcaatagatt
    12541 tgcttgacat tcgaccaaaa ttccgtcgtg ctatagcgcc tgtaggccaa gacctgttaa
    12601 ctcagtcacc tgaattttcg tgaacagagt cacgacaagg ggttgatatc cgcagagaac
    12661 gagtccatat cggtaactcg ttgccagtgg aaggtttatc aacgtgcgac gcattcctgg
    12721 aagaatcaaa ttaggtaagg tgaatatggg taaagctctt gtcatcgttg agtccccggc
    12781 aaaagccaaa acgatcaaca agtatctggg tagtgactac gtggtgaaat ccagcgtcgg
    12841 tcacatccgc gatttgccga ccagtggctc agctgccaaa aagagtgccg actctacctc
    12901 caccaagacg gctaaaaagc ctaaaaagga tgaacgtggc gctctcgtca accgtatggg
    12961 ggttgacccg tggcacaatt gggaggcgca ctatgaagtg ttgcctggta aagagaaggt
    13021 cgtctctgaa ctgaaacaac tggctgaaaa agccgaccac atctatctcg caaccgacct
    13081 tgaccgcgaa ggggaagcca ttgcatggca cctgcgggaa gtgattgggg gtgatgatgc
    13141 gcgctatagc cgagtggtgt ttaacgaaat tactaaaaac gcgatccgcc aggcatttaa
    13201 taaaccgggt gagctgaata ttgatcgtgt taatgcccag caggcgcgtc gctttatgga
    13261 ccgcgtggtg gggtatatgg tttcgccgct gctatggaaa aagatcgctc gtggtctgtc
    13321 tgccggtcgt gtgcagtcgg tggcggttcg cctggtggtc gagcgtgagc gtgaaattaa
    13381 agcgttcgtg ccggaagagt tctgggaagt cgatgccagc acgaccacgc catctggtga
    13441 agcgttggcg ttacaggtga ctcatcagaa cgacaaaccg ttccgtccgg tcaacaaaga
    13501 acaaactcag gctgcggtaa gtctgctgga aaaagcgcgc tacagcgtgc tggaacgtga
    13561 agacaaaccg acaaccagta aacctggcgc tccttttatt acctctacgc tgcaacaagc
    13621 tgccagcacc cgtcttggat ttggcgtgaa aaaaaccatg atgatggcgc agcgtttgta
    13681 tgaagcaggc tatatcactt acatgcgtac cgactccact aacctgagtc aggacgcggt
    13741 aaatatggtt cgcggttata tcagcgataa ttttggtaag aaatatctgc cggaaagtcc
    13801 gaatcagtac gccagcaaag aaaactcaca ggaagcgcac gaagcgattc gtccttctga
    13861 cgtcaatgtg atggcggaat cgctgaagga tatggaagca gatgcgcaga aactgtacca
    13921 gttaatctgg cgtcagttcg tagcctgcca gatgacccca gcgaaatatg actccacgac
    13981 gctgaccgtt ggtgcgggcg atttccgcct gaaagcacgc ggtcgtattt tgcgttttga
    14041 tggctggaca aaagtgatgc ctgcgttgcg taaaggcgat gaagatcgca tcttaccagc
    14101 agttaataaa ggcgatgctc tgacgctcgt tgaacttaca ccagcccagc actttaccaa
    14161 gccgccagcc cgtttcagtg aagcatcgct ggttaaagag ctggaaaaac gtggtatcgg
    14221 tcgtccgtct acctatgcgt cgatcatttc gaccattcag gatcgtggct acgtgcgagt
    14281 agaaaatcgt cgtttctatg cggaaaaaat gggcgaaatc gtcaccgatc gccttgaaga
    14341 aaatttccgc gagttaatga actacgactt taccgcgcag atggaaaaca gcctcgacca
    14401 ggtggcaaat cacgaagcag agtggaaagc tgtactggat cacttcttct cggatttcac
    14461 ccagcagtta gataaagctg aaaaagatcc ggaagagggt ggtatgcgcc cgaaccagat
    14521 ggttctgacc agcattgact gcccgacttg tggtcgcaaa atggggattc gcacagcgag
    14581 caccggggta ttccttggct gttctggcta tgcgctgccg ccgaaagagc gttgcaaaac
    14641 caccattaac ctggtgccgg aaaacgaagt gctgaacgtg ctggaaggcg aagatgctga
    14701 aaccaacgcg ctgcgcgcaa aacgtcgttg cccgaaatgc ggcacggcga tggacagcta
    14761 tctcatcgat ccgaaacgta agttgcatgt ctgtggtaat aacccaacct gcgacggtta
    14821 cgagatcgaa gagggcgaat tccgcattaa aggttatgac ggcccgatcg ttgagtgtga
    14881 aaaatgtggc tctgaaatgc acctgaaaat ggggcgtttc ggtaaataca tggcctgcac
    14941 caacgaagag tgtaaaaaca cacgtaagat tttacgtaac ggcgaagtgg caccaccgaa
    15001 agaagatccg gtgccattac ctgagctgcc gtgcgaaaaa tcagatgctt atttcgtgct
    15061 gcgtgacggt gctgccggtg tgttcctggc tgccaacact ttcccgaaat cgcgtgaaac
    15121 gcgtgcgcca ctggtggaag agctttatcg cttccgcgac cgtctgccgg aaaaactgcg
    15181 ttatctggcc gatgcgccac agcaggatcc ggaaggtaat aagaccatgg ttcgctttag
    15241 ccgtaaaacc aaacagcaat atgtctcttc ggaaaaagac ggaaaggcga ctggctggtc
    15301 agcattttat gttgatggca aatgggttga aggaaaaaaa taacctttaa ttctgtcagg
    15361 tttttataaa caaagggtcg cgaaagcggc ccttttttat tgcatattat tttttcttca
    15421 aacctataca ctaaggctat aaatgatata gtggttatag ttagcacctt ttttattatt
    15481 aaatcgtatt agtcacccgc caggtgtgac gaaaaaacga tgttctgatg gcgtctaagt
    15541 ggatggttta acatgaaatt acaacaactt cgctatattg ttgaggtggt caatcataac
    15601 ctgaatgtct catcaacagc ggaaggactt tacacatcac aacccgggat cagtaaacaa
    15661 gtcagaatgc tggaagacga gctaggcatt caaatttttt cccgaagcgg caagcacctg
    15721 acgcaggtaa cgccagcagg acaagaaata attcgtatcg ctcgcgaagt cctgtcgaaa
    15781 gtcgatgcca taaaatcggt tgccggtgag cacacctggc cggataaagg ctcgctgtat
    15841 atcgccacca cgcataccca ggcacgctac gcgttaccaa acgttatcaa aggctttatt
    15901 gagcgttatc cccgcgtttc tttgcatatg caccagggct cgccgacaca aattgctgat
    15961 gccgtctcta aaggcaatgc tgatttcgct atcgccacag aagcgctgca tctgtatgaa
    16021 gatttagtga tgttaccgtg ctaccactgg aatcgggcta ttgtagtcac tccggatcac
    16081 ccgctggcag gcaaaaaagc cattaccatt gaagaactgg cgcaatatcc gttggtgaca
    16141 tataccttcg gctttaccgg acgttcagaa ctggatactg cctttaaccg cgcagggtta
    16201 acgccgcgta tcgtcttcac ggcaacggat gctgacgtca ttaaaactta cgtccggtta
    16261 gggctggggg taggggtcat tgccagcatg gcggtggatc cggtcgccga tcccgacctt
    16321 gtgcgtgttg atgctcacga tatcttcagc cacagtacaa ccaaaattgg ttttcgccgt
    16381 agtactttct tgcgcagtta tatgtatgat ttcattcagc gttttgcacc gcatttaacg
    16441 cgtgatgtcg ttgatgcggc tgtcgcattg cgctctaatg aagaaattga ggtcatgttt
    16501 aaagatataa aactgccgga aaaataattt tttgcgttat tttcggcacc ttttatgtag
    16561 cgaaggtgcc gga
//
LOCUS       DAZFRN010000110        16509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFRN010000110 DAZFRN010000000
VERSION     DAZFRN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.112"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..506
                     /gene="cbi"
                     /locus_tag="ACHKUB_003428"
     CDS             <1..506
                     /gene="cbi"
                     /locus_tag="ACHKUB_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin B immunity protein"
                     /protein_id="HFO6656401.1"
                     /translation="KKANEILYAFSIIGIIPLMAILILRINNPYSQVLYYLYNKVAFL
                     PSITSLHDPVMTTLMSNYNKTAPVMGILVFLCTYKTREIIKPVTRKLVVQSCFWGPVF
                     YAILIYITLFYNLELTTAGGFFKLLSHNVITLFILYCSIYFTVLTMTYAILLMPLLVI
                     KYFKGRQ"
     gene            complement(524..2059)
                     /gene="cba"
                     /locus_tag="ACHKUB_003429"
     CDS             complement(524..2059)
                     /gene="cba"
                     /locus_tag="ACHKUB_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin B"
                     /protein_id="HFO6656402.1"
                     /translation="MSYNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVRSNPEILEDGVTSETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISAVEILDSNSLSQRQEAENNAKDDFRVKKEQENDEKTVLTKTSEVIISVGD
                     KVGEYLGDKYKALSREIAENINNFQGKTIRSYDDAMSSINKLMANPSLKINATDKEAI
                     VNAWKAFNAEDMGNKFAALGKTFKAADYAIKANNIREKSIEGYQTGNWGPLMLEVESW
                     VISGMASAVALSLFSLTLGSALIAFGLSSTVVGFVGVVIAGAIGAFIDDKFVDELNHK
                     IIK"
     gene            2245..2733
                     /locus_tag="ACHKUB_003430"
                     /pseudo
     CDS             2245..2733
                     /locus_tag="ACHKUB_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2871..3146)
                     /locus_tag="ACHKUB_003431"
     CDS             complement(2871..3146)
                     /locus_tag="ACHKUB_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6656403.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(3140..3784)
                     /locus_tag="ACHKUB_003432"
     CDS             complement(3140..3784)
                     /locus_tag="ACHKUB_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6656404.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAAKAEIEHLMNEVFGPW"
     gene            4013..4984
                     /gene="parM"
                     /locus_tag="ACHKUB_003433"
     CDS             4013..4984
                     /gene="parM"
                     /locus_tag="ACHKUB_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFO6656405.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKISKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLVPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLIADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            4989..5381
                     /locus_tag="ACHKUB_003434"
     CDS             4989..5381
                     /locus_tag="ACHKUB_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6656406.1"
                     /translation="MDEERKRKKFTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGALQ
                     SAEKMPPSATDSVQEAMNDVRLKMQKLF"
     gene            complement(5386..6657)
                     /locus_tag="ACHKUB_003435"
     CDS             complement(5386..6657)
                     /locus_tag="ACHKUB_003435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO6656407.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGCRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCQQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGNVWFA
                     GRGTAPEWQMKREMLSPSYTTQWTEIPIASF"
     gene            complement(6657..7094)
                     /gene="umuD"
                     /locus_tag="ACHKUB_003436"
     CDS             complement(6657..7094)
                     /gene="umuD"
                     /locus_tag="ACHKUB_003436"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6656408.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRGEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(7091..7339)
                     /locus_tag="ACHKUB_003437"
     CDS             complement(7091..7339)
                     /locus_tag="ACHKUB_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6656409.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            7757..8659
                     /locus_tag="ACHKUB_003438"
     CDS             7757..8659
                     /locus_tag="ACHKUB_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6656410.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKNMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(8610..8840)
                     /locus_tag="ACHKUB_003439"
     CDS             complement(8610..8840)
                     /locus_tag="ACHKUB_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFO6656411.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRH
                     PLPEKKRAVARPSPSYFIRHNAPPCRLSTQVR"
     gene            8756..8968
                     /locus_tag="ACHKUB_003440"
     CDS             8756..8968
                     /locus_tag="ACHKUB_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656412.1"
                     /translation="MNAACGQCPASGRARDGRFYRSRLASVTVYASPSPFSDERPSSR
                     FRGIALPSERRRLRYSTVGLTRYRTR"
     gene            9045..9728
                     /locus_tag="ACHKUB_003441"
     CDS             9045..9728
                     /locus_tag="ACHKUB_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6656413.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            9729..9950
                     /locus_tag="ACHKUB_003442"
     CDS             9729..9950
                     /locus_tag="ACHKUB_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656414.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            9931..10398
                     /locus_tag="ACHKUB_003443"
     CDS             9931..10398
                     /locus_tag="ACHKUB_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6656415.1"
                     /translation="MRALKIEKETRMYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQ
                     ALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGAAMEIIKYVSENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            10443..11213
                     /locus_tag="ACHKUB_003444"
     CDS             10443..11213
                     /locus_tag="ACHKUB_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656416.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVQ
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVFDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQMRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            11629..12054
                     /locus_tag="ACHKUB_003445"
     CDS             11629..12054
                     /locus_tag="ACHKUB_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6656417.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            12101..12520
                     /locus_tag="ACHKUB_003446"
     CDS             12101..12520
                     /locus_tag="ACHKUB_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6656418.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            12520..12711
                     /locus_tag="ACHKUB_003447"
     CDS             12520..12711
                     /locus_tag="ACHKUB_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656419.1"
                     /translation="MNISKETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            13749..13979
                     /locus_tag="ACHKUB_003448"
     CDS             13749..13979
                     /locus_tag="ACHKUB_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656420.1"
                     /translation="MTVSIVSPSAAAVKPRRHTRFRREDIPAPEIDPVLKAFGRHIAR
                     SFRRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            14031..15392
                     /locus_tag="ACHKUB_003449"
     CDS             14031..15392
                     /locus_tag="ACHKUB_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO6656421.1"
                     /translation="MTLSAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTISQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            15439..16002
                     /locus_tag="ACHKUB_003450"
     CDS             15439..16002
                     /locus_tag="ACHKUB_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO6656422.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELLRGTFAYTDVPTMIIRLRA"
     gene            16002..16244
                     /locus_tag="ACHKUB_003451"
     CDS             16002..16244
                     /locus_tag="ACHKUB_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656423.1"
                     /translation="MTQGWREDKCPSVRAGKKPRAGGAERGRAHAPPLSLRGRSDGGF
                     CLFRPVICLLLMMLLFLLLLLLLLLLLLLLFFFLPL"
     gene            complement(16318..>16509)
                     /locus_tag="ACHKUB_003452"
     CDS             complement(16318..>16509)
                     /locus_tag="ACHKUB_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656424.1"
                     /translation="YIPAAFCRDARSAGHGATEAARPERSLQTRGFHASDRSDSPKPR
                     QRVRRRLAGAPAAACRSRA"
BASE COUNT         3789 a         4251 c         4382 g         4087 t
ORIGIN      
        1 acaagaaagc aaacgaaata ttatatgcat tttccataat cgggattatt ccattaatgg
       61 ctatattaat acttcgaata aataatccat attctcaagt gctgtactac ttatataata
      121 aggtggcatt cctcccttct attacatcat tgcatgatcc cgtcatgaca acacttatgt
      181 caaactacaa caagacagca ccagttatgg gtattctcgt ttttctttgc acatataaga
      241 caagagaaat cataaagcca gtaacaagaa aacttgttgt gcaatcctgt ttctgggggc
      301 ccgtttttta tgccattctg atttatatca cactgttcta taatctggaa ctaacaacag
      361 caggtggttt ttttaaatta ttatctcata atgtcatcac tctgtttatt ttatattgtt
      421 ccatttactt tactgtttta accatgacat atgcgatttt actgatgcca ttgcttgtca
      481 ttaaatattt taaagggagg cagtaacact gccttccttt taattatttt atgatcttgt
      541 gattcaactc atcaacaaat ttatcatcga taaatgcacc aatagcacct gcaataacta
      601 cgccaacaaa accaacaact gtggacgaaa gaccaaaggc tataagggcc gagcctaatg
      661 tcaaagaaaa caaactaaga gctacagcag atgccatccc actgataacc caggactcga
      721 cttccagcat taatggcccc cagttaccag tctggtaacc ctcaatactc ttctccctga
      781 tgttgtttgc ctttattgca taatctgctg ctttgaacgt tttacccaac gcagcaaatt
      841 tattccccat atcctcagca ttaaatgctt tccacgcatt cacaatggct tctttgtccg
      901 ttgcatttat tttaaggctg gggttagcca ttaacttatt aatggaagac attgcatcat
      961 cataactacg aatcgttttt ccctgaaaat tatttatatt ctctgcaatt tcacgggaaa
     1021 gcgccttgta tttatctcca agatattccc cgactttgtc accgacacta atgatgacct
     1081 cgctggtttt tgtcaggacc gtcttctcgt cattttcttg ttctttcttg actctgaagt
     1141 catcctttgc gttattttcg gcttcctggc gctggcttaa actattactg tccagaatct
     1201 ccacggctga aatatatagc ggagaaactc cactgccttc aggaaaacgg acaatgacat
     1261 catgattatt actggacatc gtataaccag cctcaacata attccctgtc tcagaagtaa
     1321 cgccatcttc caggatttcg ggattagacc gaactggagt attcatctga cccacactta
     1381 ataccaggtc cggtagattt ggtgatacag gggcactgta aacatcttta atattggttc
     1441 ccggacgact ccggtccgga accggaacgg catccactac tggcactgtc atcggaatac
     1501 ctccgataat agccatatat tgtgtaccat tctgcataag gtccgtcaga cgatatttta
     1561 ccggtacatt attacccgct gctggtaact ggctggcagg gatatccgtt acctgctcca
     1621 gttgctgtgt aaccataaga cgaataattt tttgctcatt tccggaagca ggctccagac
     1681 gggcaaaaga aacagcagcc gggcgagacg gttctggcaa accaccacgt aaaccatttt
     1741 caagaactgt tcgtgcagcg gaaactttct cgtttcccca ggggctatgc acactgacag
     1801 accatcccga ccagtctccc tcttcaagtt ctttatcaag aataatccgg cgaataattc
     1861 caggaaaaag aacactttgt acaagtgcga taccttgtgg gctgtaatcc ccccaccccg
     1921 gaggcatgga tggagcgaga cctgatgagc ctccgggttt cacatcaccg cccggaattc
     1981 gtcctgttga cggccacaca accattgtgt ccccgtaatc aataccttct gtcggtacac
     2041 taccttcatt ataactcata gaaaacctct ttgactttaa aaacaatcaa ttaaaattaa
     2101 atactgtaca tataaccact ggttttatgt acagataaaa aattacgccg ctgtgttttc
     2161 tctgtcaaga ctgtggattt caatttttgt gaaaacgatc aaaaaacagg cactcacacc
     2221 acgtgctatt ctcgcctgat accacaaaaa actgcacaag cattactggt caggacggca
     2281 ggaagaatgc ccgccggcca gcagactcac cggctggttc cgctctcgga ctcctggtac
     2341 gtcagccagc tgcagacgat agtggccaca ctgaaaatcc ccatggaacg gcgtaataaa
     2401 cgaacaggcg ggacagagaa agcgcggatc tgggaagtga cggacagaac ggtcaggacc
     2461 tggattgggg aggcggttgc cgccgctgcc gctgatggtg tgacgttctc tgtcccggtc
     2521 acgccacata cgttccgcca ttcctatacg atgcacatgc tgtatgccgg tataccgctg
     2581 aaggtcctgc agagcctgat ggggcataag tccatcagct caacggaagt ctacacgaag
     2641 gtgtttgcgc tggatgtggc tgcccggcac cgggtgcagt tttcgatgcc tgagtccgat
     2701 gcggtcacaa tgctgaaaaa cagacacgca taattcataa atcataatta tgaattgtga
     2761 tttattctgt aaaaaaagag accactgcaa tatgtgatct cttgtatgca agggggctta
     2821 aacagtatga attcacaagt gtgatttcat aagtaataac tttttgatta ttatgttagt
     2881 gtttttaagt attgctcaag ggctactctg acgatggcac ttacattttt tggatcttta
     2941 ccattgcgtt tattcctcaa tgccagatct tctattgtga caagcatgct ttcaccaaga
     3001 gagattgtcg tccgacattg tttttctggt tcgggttttt ccggcgcgcc gtatggttta
     3061 tccgcaaggc gctgagccag agcctctgct tgttctaccg taactgcagc tctgtttaat
     3121 gcttgctggc tgggtttctt caccatgggc caaatacctc attcattaaa tgttcaattt
     3181 ctgctttggc tgctgtattg ttcgtttcta ctacaccggt gccgttgctc atgcaatcgc
     3241 gatagacttt tctgaaacaa ataactgaat ccagaacttg aattgttggg aattcttcaa
     3301 gatattcaag gaattctttt ctctcatttc ccctcaaaac cggattggtt gttgccatac
     3361 tttgtaaaca ataaacctta agttccgggt taaggttacg cattgcgtct atctgttgtt
     3421 ctagttctgt aagagtatcc agatctggtt gagaacactg tagtggcgca attatttgat
     3481 gtgcgacaac tccactcgtt atgaattcct ttgagtttct tcctgcgaca tccactatta
     3541 catcatcgaa cttttcattc agagcctgta gtgtttgtgt cagattatca aatttctcaa
     3601 caagagttat tgctggtaaa agtcctgcag cttctcgttc cgcatgatgt tttgcggctg
     3661 ttcgttgtag atctccgttt aacaggcaga cttcccttcc tttatttgcg agagcaatag
     3721 ccaggtttga tgcaatggta gttttccctg aaccaccttt gttcccacca actacagtaa
     3781 tcattgcaac ctcataaatg tgatgtgtga agtatgatga tattttgaca cggtaacctg
     3841 aggtgtcaat atgtgttcat atttatgaag tatgaaacat gaagtgtgtc atatgatgta
     3901 ataaataata cttgtgtttt gtgatttcat aactatgata gtacccaaat tgagtattac
     3961 cttgcaacca gaaacagtga tttactcaga ttgagtattt aaggggctat agatgaacgt
     4021 atactgcgat gatggttcaa caacaatcaa gttggcatgg aatgacaatg ggaaaatcag
     4081 taagtcgttg tcgcaaaact catttcgtca tggatggaag gttgacgggt tagggattcg
     4141 ccagacattt aactatgaac tggatggtaa aaaatacacg tatgatgaag ttagcaacca
     4201 atctattctt acgactcata ttgagtatca atatacggat gttaacctgt tagctgtgca
     4261 tcacgcttta ctgaatagtg gattagtgcc tcagcctgta tcattgactg tgactttgcc
     4321 tatcagtgag ttctacacga aagagtgtca aaaaaatgaa ctcaacattc agcgtaaaat
     4381 tgagaattta atgaggccga ttcgtcttaa taaaggcgat gttttcacca ttgaacacgt
     4441 tgatgtgatg cctgaatcat tgccagctgt gttttctcgt ctggtcatgg ataaagttgg
     4501 tcagttcgaa aaatcgctgg tagttgatat tggcggcact acgcttgatg ttggtgttat
     4561 cgttggtcaa tttgactctg taagtgccat tcatggtaat tctggcattg gtgtgtcttc
     4621 agttacgaaa gctgcaatga gtgcattgcg catggcatcc agtgatacca gtttcctcat
     4681 tgcagatgaa ttgattaaac gtcgtaatga cccggacttt gttcggcagg ttataaatga
     4741 cgaaacaaaa actgatctgg tccttaatac cattgagggg gctattgcca gtctggggga
     4801 gcaagtagtc aatgagctcg gtgattttca tcatgtgaat cgtgtttatg ttgtcggtgg
     4861 tggggccccg ttgatctatg attcgataaa aacggcatgg caccatcttg ggcaaaaagt
     4921 agtgatgatg gagtcgccac agacagcact ggttgaagct atcgcggctt tcaaagagga
     4981 gtaatattgt ggacgaggaa cggaaaagga aaaaattcac actttatctg catccggaaa
     5041 aagctgcaga ctttcaaact ttggaagcga tagagtccgt tccgcgttct gagcgtggtg
     5101 agctgttccg gaatgcattt atttctggaa tggcgttaca ccaacttgat cctcgcctgc
     5161 ctgtattgct gactgccatt ttgagcgagg agttttcagc agatcaggtt gtaaccttac
     5221 tcagtcagac gactggatgg aagccttctc aggccgatat cagagctgtg ttgactgaac
     5281 tcggtgcttt gcaatctgct gaaaaaatgc ctccttctgc tactgattcg gtgcaggagg
     5341 caatgaatga tgtgcgcctt aaaatgcaaa aattattctg atatatcaga atgaagcaat
     5401 ggggatctca gtccattgtg ttgtatacga aggcgagagc atttcccgtt tcatttgcca
     5461 ttcaggggcg gttcctcgcc ccgcaaacca cacattccct tttccagact ggttcagttc
     5521 atcgagtgtt ttcattaact tttcgctgtt tttacggggc tggatctcat caaataatcc
     5581 cggctgcgct atacccgatg gtgtgaaatc agccagcatg actcctgcct tcatatagcg
     5641 gtacccttca cgccagacat ggtttaacgc tctgcatgct gcggcaataa tgtcccggct
     5701 gtcctgtgtg ggcaatggaa gcttttccac agcggcattg ctgtaacagg gttcttttac
     5761 tgcaaagggg gatgtccgta caaatgtcgt cacctgctgg caatactgac gctccccacg
     5821 tagtttctct gcagcccgct ctgcatactg aacaacagcc tggtgcatgg catctttgtc
     5881 tgtgattcgt tcaccaaaac tgcgactaca gacaatctgc tgttttgccg gtggtgcttc
     5941 ttccagggat atgcaggact cgccgttgag ttcgcgtacc gtacgctcaa gaatgacgct
     6001 gaagtttttc cggatgaatg ccgtgttagc ctgcgccagc tgcagtgctg tgttaatacc
     6061 cagcgcattc agcttttccg tcagtctgca tcctactccc cagacctcac caactggctg
     6121 cagccccagt agcttcaaga tccgattacg gttttctgcc gtcagcgcga ccacaccgga
     6181 aaactgtggc cattgctttg ttgcccactg tgcactttta gccagcgttt ttgtaggcgc
     6241 aatgcccacc cccatggtga gtcctgtcca gctctttacc tgttccctga gctgatgacc
     6301 aaaaaactcc ggagagatgc aatgatttat cccccgcaaa tcaataaaca tttcatcaat
     6361 tgagtagggc tcaactgcgg gagaaagcga ctccagaaca gccatgaccc gttggctcat
     6421 gctgtggtac agcgcataat tgctggaaaa tacatgtatt ttcttctcca ggcgcatctg
     6481 tctcacctga aaccagggct gccccattct gatgccaaga gcttttgcct ccgggctgcg
     6541 cgcgatcaca cagccatcgt tattgctgag tacgatgacc ggttcgttgc gaaggtccgg
     6601 gcggaaaact ttttcacatg aggcgtagaa actgttgata tcagccagtg caaacatcag
     6661 cgtaactccc gcgtcctgtg tatcacgtga gtgacaacac caaaaataca gatgttttcc
     6721 ggatacagtg tgcggaattc cgggctgtct gaaaccggct ccagtgccgg gcgtgggcgc
     6781 aacagcagtc gtttgacggt gaactcaccg tcgatctcag cgataacgat gtccccgtgt
     6841 tgtggttttt cgcccctgtc tactaccagc agatcaccat tctgcacgcc agcctggttc
     6901 atcgattcac cgctggcgcg cagaaagaag gtggctgcag gtctgctgat gcaatagctg
     6961 ttcagatcca gttcctgctc agcataatca gtggcaggcg aaggaaaacc ggcctggcaa
     7021 cgatcggcaa acaacggtcg cacgtaactg tcatcgcctg aagggtcagc cggacggtga
     7081 taaacggtac tcatgctgca ggcagccagc tgtcgtcctg ccagatttct tccagcagag
     7141 acaggatacg ttctttatcc ttgtcgttgc gggctcctgt gacagataca gacggggcgc
     7201 tgccctggtc tatacgcagc cagatttccg ggtagtgtgg tttcagacgt tgttcgattt
     7261 cattctgtag cgcctgaagt gtgcctgatt tgaggttttt tgtgctctgg cggtcgaaaa
     7321 gaatttcaat gcggatcatg ttctcatctt ccttttcttt tgctgtatgc atatacagta
     7381 ttctaataac tgaatccgca cacagtcaaa tgtcattgtt ctgattaaaa aacgctcaat
     7441 tctctgaggg tgtggctgta tgcctgctgt gaagtgctgt gtccagctta tccacagcat
     7501 tttgtgcacg gttctgtgga caaaatacct ggttacccag gccgtgccgg cacgttaacc
     7561 gggctgcatc cgatgcaagt gtgtctctgt cgccggcctc ctcacccggt cacgtttcgt
     7621 cgttcctcct ccacgagctg cggcttcggg gccgcacctg cattcgtatg cggtcgcccg
     7681 gttacaggtg cggcacggcc tgatggaggc cgcatgtaag aggagaattc ccatgccaaa
     7741 ctggtgctca aatcgtatgc atttttctgg tgaaccggca cagattgctg agattaaacg
     7801 actggccagc ggtgcagtca caccgcttta tcgccgcgcc acaaatgaag gtattcagct
     7861 gtttctggcc ggaagtgccg gacttctgca gaccactgaa gatgtgtggt ttgaaccgtg
     7921 ccccggactg acggctgccg gacgtggcgt tgtatcgccg gagaatatcg cgttcacccg
     7981 ctggctgaca cacctgcagg acggtgtact gctggatgaa caaaactgcc tgatgctgca
     8041 tgaactctgg ctgcagagcg gtactggcca gcgtcgctgg gaaggattac cggacgatgt
     8101 cagggatacc atcaccgcgc ttttcaccgc aaaaagaggt gactggtgtg gcttctggag
     8161 taacgaggat gtatcggtgt ggtggaaccg tctgtgtgac aacgtactgc cggaaaaaaa
     8221 catgccgttt gacctgctga cggttctgcc cacccgcctt gatgttgaag tgaatggctt
     8281 taacggtggt gttctgaacg gtgttccttc tgcatatcac tggtataccg aacagtatgg
     8341 cgtgaagtgg ccttgtgggt atgacctgaa tatcagcagt cagggagaca acttcattca
     8401 ggttgatttc gacacgccgt ggtgtcagcc ggaaagcgac gttattgcag aattaagccg
     8461 ccgtttcagc tgcacgctgg agcactggta tgccgaacag ggctgtgatt tctgtggctg
     8521 gcagttgtat gagcgcggag agctcgttga tgtgctgtgg ggggagctgg aatggtcttc
     8581 cccgacagat gacgatgagc tgccggaagt caccggacct gcgtggatag tcgacaaggt
     8641 ggcgcattat ggcggatgaa gtatgacgga gacgggcggg ctaccgcccg tttcttttcc
     8701 ggtaaaggat gtcgccgtgc tcctcttttt actgcgccgg ctgacgcggc gcggcataaa
     8761 cgctgcctgt ggtcagtgtc ccgcgtccgg ccgggcacgg gacgggcgct tttaccgctc
     8821 ccggctggcg tcggtgacag tgtacgccag cccgtcgccc ttttctgatg aacgcccttc
     8881 aagccgcttt cgcggcatag ccttgccgtc agaaagacga cggctgcggt attccacggt
     8941 cggcctgacc cgttaccgga cgcggtgaac agcccacagg cagcggggaa cgggcaccac
     9001 aggggtgccc tcccggtgtc ctctgtaaga gaaggagcgt tgttatgtcc cgttttgtcc
     9061 tcggtaactg catcgatgtt atggcccgta ttcctgataa cgccattgat ttcatactga
     9121 ctgacccgcc gtacctcgtc ggttttcgtg accgtttcgg gcgcaccatc gctggcgata
     9181 aaaccgatga atggctgcaa ccggcctgta atgaaatgta ccgcgtactc aaaaaagacg
     9241 cgctgatggt cagcttctac ggctggcacc gtattgaccg ctttatggca gcctggaaac
     9301 gcgccggatt cagcgtggca gggcaccttg tgttcaccaa aaactacaca tccaaatctg
     9361 cgtatgtcgc gtaccgccac gaatgcgctt acatcctggc aaagggcagt ccggcccggc
     9421 cccgtaaccc gctgccggat gtgctgggct ggaaatattc gggcaatcgc catcacccga
     9481 cggaaaagcc cgttaccagc ctgcaaccgc tgattgagag cttcacacac ccgaacgcaa
     9541 ttgtgctgga cccgtttgca ggcagcggct caacctgcgt cgccgccctc cagtccggac
     9601 gccggtatat cggtatcgag ctgcttgagc agtatcaccg tgccgggcag caacgccttg
     9661 ccgccgtgca acgggccatg cagcaggggg ccgcgaatga tgactggttt atgccggagg
     9721 ctgcgtaaat gaactatgcc ggacacgaaa aactgcgcgc cgaagtggcg gaggtggcca
     9781 atgccatgtg cgacctgcgt acaaccatga atgagatgga gcagcggtac agctttaatg
     9841 ccgacaccct gccggaacgt ctggtgcgtc agacgctgtt tcgcgcaaac cgcctcctga
     9901 tggaggcata taccgaaatt ctggaactgg atgcgtgctt taaagattga gaaggagacg
     9961 agaatgtacg gaacatgcga aacgctatgc cgggcgctgg cagcaaagta tccgggagac
    10021 acgccgctga tgctggttat ctggtccccg gaagaaattc aggccctcgc tgacggaatg
    10081 gatatttccc tgtccgatca tgaaatcagg actgtcctgg cgcgcctgga ggacatcccg
    10141 gaagaccagc ggactgaatc cggtatttct tccggtgcgg cgatggagat tatcaaatac
    10201 gtgagcgaaa accgccaggt gaccgtccct gctgaactgc tggcgtccct gattcagacc
    10261 gctgaacagg cactgtggaa acgtgagtgg gccgcccgtg ataacggcct cgccgttccg
    10321 gaatgcgtca cccgccgaca ggcggtggtt aatcaggccc gcgccctgct gaaaaacaac
    10381 acacgcgaaa acgactgatg ttatcgccgc ctgcgggcgg cgattcaggg agaccatgaa
    10441 tcatgaacga aacgctgaat gcactgattt gtcgccacgc ccgtaacctg ctgctggcgc
    10501 agggctggcc ggaagagacg gatgttgacc agcgaaatcc gaactatccg ggctggatca
    10561 gcatttacgt gcagctggat gcgccccggc tggcgacgtt actcgttaac cgccacgatg
    10621 gcgtgctgcc gccgcacctg gcctccgcca ttcagaaact gaccggaacc ggagcggaac
    10681 ttgtactgtc cggcagtcag tggcaggcgc tgccggtact tccggcagac ggaacgcagg
    10741 tgtctttccc gtatgccggt gaatggctga cggaggaaga aatcagggca gtttttgatg
    10801 cggtgcgcaa tgcagtacga agcatctgtt accaggtggc agacgatgcg cggcgtatcc
    10861 gggcggcgct gactaccacc ggtcagacgt tgctgacccg ccagatgcgc cgctttcgtc
    10921 tggttgtgaa ggaaagcgat catccctgct ggcttgatga agatgacgaa aatctgcccg
    10981 tggtgctcga tgccatcctg aaccggggag cacgtttttc ggcggtggaa atgtatctgg
    11041 tcagcgattg tattgagcat atcctgtcca gtgggctggc ctgcgatgtg ctgcgtatac
    11101 cggatgaacc gccccgccgg tggtttgacc gtggtgttct gcgggaggtg gtccgggaag
    11161 cccgggccga aattcgcagc atggcggatg ccctggcaaa aatccggaaa tgatgacgct
    11221 ggcggggaaa aatctccgcc ttttcctgcc ggtccccgga cagagcacaa aggatgtcgc
    11281 cgccggctcc gctttacccg gccatgcggg gcatggcctt gtgggttttc agttatgtgg
    11341 cctcagcgtc gtgtgcgggc tgtgccgtgc ctccatcgta gccgggcttc tgcgggcggg
    11401 ttgcaaggta aggtgaaccg ctgcggtcac ccggtccctc cttcccgtct gccgtgattt
    11461 tccggtcgtt cacccgctca ggtttcgcgg tctcaccctc caactcccgt cagccgtgtg
    11521 cgtcaggctg cggcttccct tgcgcccttg caaccctgca ggcccggcaa agatggaggc
    11581 acggcaccgc ccggtgtcgc ggaatatgtg aacaacggag gaaatcccat gcagtatgcg
    11641 aaacctgtca ctctgaacgt tgaagagtgc gaccgtctgt cttttctgcc ttacctgttt
    11701 ggcaacgatt ttctgtatgc cgaggcgtat gtgtacgcgc tggcgcaaaa aatgatgccg
    11761 gaatatcagg gaggcttctg gcatttcatc cgcctgccgg acggtggcgg ttacatgatg
    11821 ccggatggcg accgttttca tctggtcaac ggtgaaaact ggtttgaccg cactgtgagt
    11881 gctgatgccg caggtatcat cctgacttcc cttgtgatta accgccagtt gtggctgtac
    11941 cacgacagcg gggatgcggg actgacacac ctttaccgga tgcgcgatgc gcagttgtgg
    12001 agccacatcg aatttcaccc cgaatgcaat gcgatttacg cggcactgga ctgattaacg
    12061 gacggggcgg cgatgccgcc cctgtaaagg agacgagaac atgtactgta ctgttaaaga
    12121 aattatccgc gatgtactgg atacagacgt gccggacagt gaatgtgttt ttgccgtggt
    12181 gctgacccgc ggggatgtgc gccacatagc ccgggactgg aacctgtcag acgatgagct
    12241 ggaaaccgtc atgcagcggc tggacgatgc ttttgtatat ggcgcatgtg acagagttgt
    12301 ttccgatatt gtcaatgaac tgatggagga aaagcgcgta aaccgtcttg taacagtccc
    12361 tgcggtgctg ctggaaaaag tgatggtgat ggctggtagt gaaatttatc gcctccatgc
    12421 tgtagggagc gagaacgggg gcgacggtga cgcatttgtc agggaagaac gtgagattat
    12481 gcgggtgatg cgtcaggcgc tggacgggga gaatgggtaa tgaatatcag caaggaaacc
    12541 cgcgaaattc tgcgtaatta cagggccgtg attaatgcgc ggcgtcgtga aatggggcag
    12601 aaaccgctca ccaccgcgca gattgttgat gaaatctgtg attttgtggc gaatcagcag
    12661 gcggttttcc tcggtggtca ctacatcctt cagggcagca ggcacaggtg atacagggca
    12721 ggcggtggag gccgcctgtc agcccgcgcg aaaacccctg gcaggctggc gcctgccgga
    12781 agggatgcgg gccacgctgg cgcgtcgccc gttccccttc ccgtttgtga tgtgtctgac
    12841 tcaggagtgt ttctttctta tgttatggaa ctgttcctga tgttgttgtt gttcttttct
    12901 ctgctgctct gattctgact actgcagtgt tgtcctgtct gccgggcgca ccttcggctc
    12961 ccggctgcct ggcggcatcc ggtcgtcagg cccgactccg acaggcagct gctggcgcac
    13021 ccgccagtct ccgccgaacc cgctggcgcg gtttcgggct gtcgctacgg tccgatgcgt
    13081 gaaaacccct ggcttgcagc gaccggaacg gtcgcgccgc ccttgtcgcg ccgtggcctg
    13141 cgctcctggc atcccggcaa aaagacgccg ggatgtaagg agcaacgccg tttcgcaaac
    13201 cgcgcttcac gtcgttgcag cgggtccctg ctcattgcct gccgggtgcc acacacccgt
    13261 atgagtccct gtcgccgggg gctgatacag ccacaacctg ccgcactgac tgtccagggt
    13321 aaatgaacag cccacaaaat tttttaccca gaacccggta aaaaattttg cgttctgccc
    13381 aggacaggtg cgtcaggccg tggcagtgat gccccttgcg gcgaaggttc tcaaacgggt
    13441 gaagttcata ccacagggaa tacagcagca ggttcccgcg aaacgtcaaa accgcggctc
    13501 ccgaaactca acgggtgatg ttctttaaaa atcaggattt atttcaggcg ccggcaatac
    13561 gcatttatgt gttctgtcag catcctgctg gcaggtaatg agaacgcagg aacagaagga
    13621 ggaatacacc ggagaacgtc agacgcagat atgaaaaaag caggtcaggg acctgcttaa
    13681 tccataccgc agagactgcg cgaacagtct ttacggcttc attaattaac gtcataagag
    13741 gctaaattat gacagtaagt attgtttcac cttccgcagc ggctgtaaag ccccgtcgtc
    13801 atacccgttt cagacgcgaa gacatccccg cgccggaaat tgaccccgtg ctgaaagcct
    13861 tcgggcgaca catagcccgg agttttcgcc gggggcgtgg tgtacacatc cccgccatga
    13921 aaaacacggc cttcgggcag gtgctccgca cactcgaact gaaacgcgct tttaactgat
    13981 gaggtgagcc gccccgcgag gggcggcaac agacggaagg agaacatttt atgacattat
    14041 cagcaatcac catgaccgcc ccggaagccg ccagcccggt gcagatgtac cgcgcgacct
    14101 actcaccgga tgataacaaa cttcgcctgt atgccgtgtc acgcctcgac ccggagacgt
    14161 ataaaaaagt gcatgatgcc ggtttccgct gggcacccaa acaggcgctg tttgtggctc
    14221 cagcctggac accgggccgg gaagacgtgc tcctctcact tgccggagag attgaggatg
    14281 aagacagcac gcttgctgaa cgtcaggaag cacgggcgga gcggtttacc ggatacagcg
    14341 gaaagcgggc cagtgaatcc gcacaggcac ttgatgaagt ggaacgactg gccgcgatga
    14401 tcccgcccgg tcagcccatt cttgtggggc atcacagcga acgccgcgcc cgtcgtgatg
    14461 cgcagcgtat tgaaaacggc atgaaacgcg ccgtgatgct ctttgaacgt gcggaatact
    14521 gggaagaacg ggcgcggtcg gcactgcttc acgcgaagta taaagaacgt ccggacgttc
    14581 gctggcgtcg tatcaaaaaa atcgaagctg atttgcgcaa ggctgaaaag accatctcac
    14641 agtcgcagaa atatctgacg atgtggcggg ctgaatcgct ggatctgaat atggcaaaac
    14701 tcatcagcag tcatgaccat atcagcgcct gtttcccgct ggatacgtat ccgcgcccgg
    14761 cagaaaaaag ccagtatgaa ggaagtcgat cgttatggtc ggccctggat gatgacatca
    14821 tcaccacgga gcaggcccgc gaaattgcga tccgctgtca tgaacggcag atccagcgtc
    14881 agcaacgctg ggttaaccac tatcagaacc gcctgatcta tgagcgtgcc atgctggacg
    14941 aaagcggcgg cgtggttacc cggacacagg attttgagcc gggtggacag gttttcagcc
    15001 ggggcgagtg gctgaccatc atccgcgtga acaaaagcaa cggggcggtg agttcagtca
    15061 caacgccgaa ttacagtttt ctcgggtaca gcggcacgat gaaagtgacg cccgatcgca
    15121 ttacggacta caaagcacca tcggcagaag aggctgccgt cgccagtcag gccgcgaagc
    15181 gtccgccggt agtcaactat ccgggggaag gtttccggga aatgacaaag gcacagtggg
    15241 ccgccctgcc ccgggactgt aaggccgtgc gcagtgtggc agaagcagaa gaccacgggg
    15301 cataccgcta ccgccgcaca atggacaata atttccgcct ggtgaatgtg tatatcaccg
    15361 acatgaaaat cacggaaatc ccgcagaaat aaggtatatc cccccgggaa atccccgggg
    15421 gatgacggag ataatgacat gcacagtcag ttaagagaac gtatccggct gatgcgcgca
    15481 aggctggata acgccgcgcc ggttgctgaa atccgggctg aatctcagct ttttgtgact
    15541 cctgccccgg tctgtgatcg cctggtgacg ctggcggaaa tcagcaaccg tgatcacatt
    15601 ctggaaccct ctgccggcac cggggccata ctgcgggcga ttcgggatac cgcgccggag
    15661 gccatgtgtg atgcggtgga gattaacagc gggctggtcc ggtatctgcg ggaaaatttt
    15721 aacggtgtca gggttcagtg tggcgacttc atggaatggc agccggtgca gtattacagc
    15781 cggatcatta tgaatccgcc gttcagtcac gggcaggata tccggcatat cctgcgggct
    15841 ttttccctgt tgcgtccggg gggcgttctg gttgctgtct gtctcaacgg gccacgccag
    15901 caggagaagc tgttaccgtt ttctgacgtc cgcgaggagc tgctgcgcgg cacgtttgct
    15961 tataccgatg tcccgacgat gattattcgt ttgcgtgcct gatgacacag ggctggcggg
    16021 aggataaatg cccgtctgtc cgcgccggga aaaaaccgcg cgccggtggc gctgaaaggg
    16081 gccgcgctca cgcgccgccc ctttcattac gcggacgttc tgatgggggc ttctgtcttt
    16141 tcagacctgt catttgtctg ttactgatga tgttgttgtt cctgctactg ctactgctac
    16201 tgctactgct actgctactg ctcttctttt tcctgccgct ctgattctga ctactgcagt
    16261 gttgtcctgt ctgccgggcg ccccttcggc tcccggctgc ctggcggcat ccggtcgtca
    16321 ggcccgactc cgacaggcag ctgctggcgc acctgccagt ctccgccgaa cccgctggcg
    16381 cggtttcggg ctgtcgctac ggtccgatgc gtgaaaaccc cgggtttgca gcgaccgttc
    16441 cggtcgcgcc gcctctgtcg cgccgtggcc tgcgctcctg gcatcccggc aaaaggccgc
    16501 cgggatgta
//
LOCUS       DAZFRN010000111        16025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFRN010000111 DAZFRN010000000
VERSION     DAZFRN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.113"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            102..1337
                     /gene="moeA"
                     /locus_tag="ACHKUB_003453"
     CDS             102..1337
                     /gene="moeA"
                     /locus_tag="ACHKUB_003453"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO6656425.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            1337..2086
                     /gene="moeB"
                     /locus_tag="ACHKUB_003454"
     CDS             1337..2086
                     /gene="moeB"
                     /locus_tag="ACHKUB_003454"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO6656426.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2162..2824)
                     /gene="fsa"
                     /locus_tag="ACHKUB_003455"
     CDS             complement(2162..2824)
                     /gene="fsa"
                     /locus_tag="ACHKUB_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6656427.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQAVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            2955..3854
                     /gene="ybiY"
                     /locus_tag="ACHKUB_003456"
     CDS             2955..3854
                     /gene="ybiY"
                     /locus_tag="ACHKUB_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO6656428.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3860..6292
                     /gene="ybiW"
                     /locus_tag="ACHKUB_003457"
     CDS             3860..6292
                     /gene="ybiW"
                     /locus_tag="ACHKUB_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO6656429.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6438..7253
                     /gene="ybiV"
                     /locus_tag="ACHKUB_003458"
     CDS             6438..7253
                     /gene="ybiV"
                     /locus_tag="ACHKUB_003458"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO6656430.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7405..8670
                     /gene="ybiU"
                     /locus_tag="ACHKUB_003459"
     CDS             7405..8670
                     /gene="ybiU"
                     /locus_tag="ACHKUB_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO6656431.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(8907..10499)
                     /gene="ybiT"
                     /locus_tag="ACHKUB_003460"
     CDS             complement(8907..10499)
                     /gene="ybiT"
                     /locus_tag="ACHKUB_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO6656432.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            10718..11638
                     /gene="ldtB"
                     /locus_tag="ACHKUB_003461"
     CDS             10718..11638
                     /gene="ldtB"
                     /locus_tag="ACHKUB_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6656433.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(11697..12815)
                     /gene="ybiR"
                     /locus_tag="ACHKUB_003462"
     CDS             complement(11697..12815)
                     /gene="ybiR"
                     /locus_tag="ACHKUB_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO6656434.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTAEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(12812..13279)
                     /gene="mntR"
                     /locus_tag="ACHKUB_003463"
     CDS             complement(12812..13279)
                     /gene="mntR"
                     /locus_tag="ACHKUB_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO6656435.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            13465..13593
                     /gene="mntS"
                     /locus_tag="ACHKUB_003464"
     CDS             13465..13593
                     /gene="mntS"
                     /locus_tag="ACHKUB_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO6656436.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            13865..15448
                     /gene="opgE"
                     /locus_tag="ACHKUB_003465"
     CDS             13865..15448
                     /gene="opgE"
                     /locus_tag="ACHKUB_003465"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6656437.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(15497..16012)
                     /gene="ompX"
                     /locus_tag="ACHKUB_003466"
     CDS             complement(15497..16012)
                     /gene="ompX"
                     /locus_tag="ACHKUB_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO6656438.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         3917 a         4285 c         4067 g         3756 t
ORIGIN      
        1 gtaaaggcag gagccaacgc tacgcccgag gtggcataca gtctgtggcg tttttctgac
       61 ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat
      121 tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgtcacccca ctgaccgccc
      181 aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc
      241 cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg cgtttagccg
      301 atattgcctc caggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccgtacc
      361 atggtgaatg gcctgcgggt acctgcattc gtattatgac cggtgcgccg gtgccggaag
      421 gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta
      481 ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg
      541 cggttgtttt cccggcggga actcgcctga ctaccgctga actgccagtg attgcttcac
      601 tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg
      661 atgaactcca gttgcccggt cagccactgg gcgatggcca aatctacgat accaaccgtc
      721 tcgccgtaca cctgatgtta gaacagttgg gatgcgaggt aatcaactta gggattatcc
      781 gcgacgatcc acatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtgg
      841 tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg attcttgaag
      901 agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg ttcgcgttcg
      961 gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga
     1021 ccttctatca actggtacag cctttgctgg caaaactaag cggcaacacc gccagcggcc
     1081 tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca ggacgtcttg
     1141 atttccagcg cggcgtgctg caacgcaacg ccgacggtga acttgaagtg acgaccaccg
     1201 gacatcaggg ttcacatata tttagctcct ttagtctcgg caactgcttt atcgtgctgg
     1261 aacgcgatcg cggcaatgtg gacgtgggcg aatgggtgga agtagaaccg tttaacgcgt
     1321 tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct acaaccggca
     1381 aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct
     1441 ggtagtcggc ctcggtggac tcggctgcgc agcttcgcag tatctggcaa gcgccggtgt
     1501 cggcaacctg acgctgctcg acttcgacac ggtttcgctc tcgaatctgc aacgccagac
     1561 gctgcacagt gatgctacgg tcgggcaacc gaaggtagaa tccgcccgtg acgccatgac
     1621 gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgcttgatg acgctgaact
     1681 tgctgcgatg attgcaaaac acgatctggt actcgactgt acggataacg ttgcggtgcg
     1741 taatcaactg aacgctggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat
     1801 tcgtatggaa gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg
     1861 ccttagccgt ttgtttggcg agaacgcatt aacctgcgta gaagcaggcg tgatggcacc
     1921 gttgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaaactgc tggcaggtta
     1981 tggcaaaccg gccagcggga aaatcgtcat gtacgatgcg atgacctgtc agtttcgcga
     2041 aatgaaactg atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc cacctccagg
     2101 ccagataaga cgcggcactc cgccacgtca tcagcaattt atgaggatgc agaacgtccg
     2161 gttaaatcga cgttctgcca aacgctccca gccagtcctg ttcaaacttc gccacagcgg
     2221 catcaaccgc cggatagcta atcatctgtt gtgccacatc cagtggcagc gtaattgatt
     2281 cacatcctgc cagtaagcag tccagcgcct gacgcggggt tttgaaactc gctgccagca
     2341 ctttcgcctg cggcgcatgc attttcaata actggtgtaa gtcggtcaca gcctgaatgc
     2401 cgctaccgcc ctgagcatca atacgattaa cgtaaggcgc aacatattcc gcacctgcca
     2461 gcgccgacag cagcccttgt gctgcgccat ataccgcggt tcccagcgtt ggaatccctt
     2521 ccgcttttaa catcttaata gctgccagcc cctcggcggt caccggaact ttcaccacga
     2581 tatccgcaat aatagaacgc aatttacgtg cgtcattcac catcccttca gcagtggtag
     2641 ccattacctg ggcaaacaga cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca
     2701 caacatccag cggtttttta cccgcggcga taatgcttgg gttagtggtc acacccgcca
     2761 gcggaaaaat acgtgacagc gccttcaccg caacaacgtc tgaagtatcc agatacagtt
     2821 ccataaccat cctcaaaatg tactttttgt ctgaatgtag actatcacgg gaaaactctc
     2881 tgaacagttg acgtacatca atctaacttt cattcgaaag tattttaatg tttgaacgaa
     2941 acagagaggc aactatgatt ttcaatattc agcgctactc gacccatgat ggccccggta
     3001 tccgcacggt cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg
     3061 aaagccgcgc ccgcacgcag gatctgctgt atgacgcacg actgtgtctg gaaggctgcg
     3121 agctgtgcgc taaggccgcg ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc
     3181 atcgggaaaa gttaaccccg gagcatctga cggcgttaac cgactgctgt ccgacacagg
     3241 cattaaccgt gtgtggtgaa gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg
     3301 ataaaccgtt ttacgatcgc agcggcggcg gtttaacgct ttcgggtggt gaacccttta
     3361 tgcagccgga aatggcgatg gcgctactgc aagccagcca cgaggcaggc attcatactg
     3421 cggtagaaac ctgtctgcat gtgccgtgga aatatatcgc cccttctctg ccctatatcg
     3481 atctgtttct tgccgattta aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg
     3541 gtaacgccgc cagagtgctg gataacctga aaaaactcgc cgcagcgggc aaaaaaatca
     3601 ttatccgcgt gccgctgatt cagggcttta atgccgacga aacctctgta aaagccatta
     3661 ccgattttgc cgccgacgag ctacacgttg gcgaaattca tttcctgccc taccacacgc
     3721 tgggcatcaa caaatatcac ttacttaatc tgccctatga cgccccggaa aaaccgcttg
     3781 atgcgccaga actgctcgac tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga
     3841 ccttacgagg ataacaatca tgacaacact gaaactggac acgctcagcg accgcattaa
     3901 agcgcacaaa aatgcgctgg tgcatattgt gaaaccgccg gtttgtaccg agcgcgcgca
     3961 gcactatacc gagatgtatc aacaacatct cgataagccg atccctgtac gtcgcgcgct
     4021 ggcgctggca catcacctgg cgaatcgcac catctggatc aaacacgatg agttgatcat
     4081 tggcaaccag gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg
     4141 gatcgaaaaa gagattgatg atctggcgga tcgtccgggt gctggttttg cggtgagcga
     4201 agagaacaaa cgcgttctgc atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga
     4261 tcgctgctac ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa
     4321 agcggaaggc aatatgacct ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct
     4381 ggaaaaaggt cttgatggtc tgcgtgagaa agtagcggaa cgtcgctcgc gcatcaatct
     4441 gacggtgctg gaagatttac atggcgaaca attcctgaaa gcgattgata tcgtgctggt
     4501 ggcagtcagt gaacacattg aacgtttcgc tgccctggcg cgtgaaatgg ctgctaccga
     4561 aactcgcgaa agccgtcgcg atgaactgct gacgatggca gaaaactgcg atcttatcgc
     4621 ccaccagccg ccgcagactt tctggcaggc gctgcaactg tgttacttca tccaattgat
     4681 tttgcagatc gaatctaacg gtcactcggt atcgtttggt cgtatggacc agtatctcta
     4741 cccgtactat cgccgcgacg ttgaactcaa ccagacgctg gatcgcgaac acgccatcga
     4801 gatgctgcat agctgctggc tgaaactgct ggaagtgaac aagatccgct ccggctcaca
     4861 ctcaaaagcc tctgcgggaa gtccgctgta tcagaacgtt accattggcg gacaaaatct
     4921 ggttgatggt caaccaatgg atgcagtgaa tcccctctct tacgcgatcc tcgaatcctg
     4981 cggtcgcctg cgttccactc agcctaacct cagcgtgcgt taccatgcag gaatgagtaa
     5041 tgatttcctc gacgcctgcg tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa
     5101 caacgacgaa atcgtgatcc cggaatttat taaactcggt attgaaccgc aggacgctta
     5161 cgactacgca gcgattggtt gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg
     5221 taccggcatg agctttatca acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg
     5281 tgatgccacc agcggcaaag tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt
     5341 taacaatttc gatgaagtaa tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa
     5401 atcaatcgaa atcgaatatg tcgtcgacac catgctggaa gagaacgtac acgatattct
     5461 ctgctcggcg ctggtggatg actgtattga gcgagcgaaa agtatcaagc aaggcggcgc
     5521 gaaatatgac tgggtttctg gcctgcaggt tggcattgcc aacctcggca acagcctggc
     5581 ggcagtgaag aaactggtat ttgaacaggg cgcgattggt cagcaacagc tggctgccgc
     5641 actggcggat gacttcgatg gcctgactca tgagcaactg cgtcagcggc tgattaacgg
     5701 tgcgccgaag tacggcaacg acgatgatac cgtcgatacg ctgctggctc gcgcttatca
     5761 gacctatatc gacgaactga aacagtacca taacccgcgc tacggtcgtg gtccggttgg
     5821 cggcaactat tacgcgggta cctcgtctat ctccgctaac gtaccgtttg gcgcgcaaac
     5881 tatggcaacg ccggacgggc gtaaagccca caccccgctg gcagaaggcg ctagtccggc
     5941 ctctggtact gatcatcttg gccctactgc ggtcattggc tcggtgggta aactgcctac
     6001 ggcagcgatt ctcggcggcg tgttgctcaa ccagaaactg aatccagcaa cactggagaa
     6061 cgaatctgac aagcagaaac tgatgatcct gctgcgcacc ttctttgaag tgcataaagg
     6121 ctggcatatt cagtacaaca tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc
     6181 ggatcagtac cgcgatctgg tagtgcgtgt cgcgggctat tccgccttct tcaccgcgct
     6241 ctctccagac gcacaggacg atatcatcgc ccgtactgaa catatgctgt aatctcctcg
     6301 gcccggcggc gtcgtcgccg ggctaaatca ttcacatcat caatttcatc cttactttca
     6361 ttcgaaatat aatttgtgct ctgcgtcaca ttgttattac actgttacgg tttcattttc
     6421 gagccaggag cacacctatg agcgtaaaag ttatcgtcac agacatggac ggtacttttc
     6481 ttaacgacgc caaaacgtac aaccaaccac attttatggc gcaatatcag gaactgaaaa
     6541 agcgcggcat taagttcgtt gtcgccagcg gtaatcagta ttaccagctt atttcgttct
     6601 ttcctgagct aaaggatgag atctcttttg tcgcggaaaa cggcgcactg gtttacgaac
     6661 atggcaagca actgttccac ggcgaactga cccgacatga atcgcggatt gttattggcg
     6721 agttgctaaa agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca
     6781 gcgaaaatgc ccccgaagca tttgtcgcac tgatggcaaa acactaccat cgcctgaaag
     6841 ctgtaaaaga ttatcaggag attgacgacg tactgttcaa gttttcgctc aacctgccag
     6901 atgaacaaat cccgttagtg atcgacaaac tgcacatagc gctcgatggc attatgaaac
     6961 ccgtcaccag tggttttggc tttatcgacc tgattattcc cggtctacat aaagcaaacg
     7021 gtatttcgcg gttactgaaa cgctgggatc tgtcaccgca aaatgtggta gcgattggcg
     7081 acagcggtaa cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg
     7141 ctgcggaaaa cattaaacaa atcgcccgtt acgctaccga tgataataat catgaaggcg
     7201 cgctgaatgt gattcaggcg gtgctggata acacatcccc ttttaacagc tgacctctct
     7261 accggagccg tgcgctccgg ttttcccttc tttttacatc ctgtacatca catcaaatca
     7321 ttaaacttgc attaacttat ttataactta tattgtcaca tgcaaatcag aatggtttcg
     7381 aatgaaagtt aatgagaggt ggttatggct tctactttta ccagcgacac attgcctgcc
     7441 gatcacaaag cagctatccg tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc
     7501 cagcagatct ttaatcagct aagcgatgac attgccacgc gagtggctga aatcaacgca
     7561 ctcaaagcac agggcgatgc cgtctggccg gtgctgtctt atgccgatat caaagcaggt
     7621 catgttactg cagagcagcg cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc
     7681 catttccccc gcgaacaagc gctaggctgg gatcagtcga tgctggacta tctggaccgc
     7741 aaccgctttg acgaggtcta caaaggcccc ggcgataatt tcttcgggac gctcagcgct
     7801 tcacgtcccg agatttaccc catctactgg tcgcaggcgc aaatgcaggc ccgccagagt
     7861 gaagaaatgg cgaatgcgca gtcgtttctc aatcgtctgt ggacatttga aagtgatgga
     7921 aagcaatggt ttaacccgga tgtgagcgtc atctaccctg accgtatccg ccgccgtccg
     7981 cccggaacga cctccaaagg tcttggagcg cataccgact ccggggcgct ggaacgctgg
     8041 ctgcttccag cgtatcagcg cgttttcgcc aacgtcttta atggcaatct ggcgcaatat
     8101 gatccctggc atgcggcaca tcgtacggaa gttgaagagt acacggtgga caacaccacc
     8161 aaatgttccg tgtttcggac attccagggc tggacagcgc tctctgatat gctgcctggt
     8221 caggggttgc tgcacgttgt gcccattcct gaagccatgg cgtacgtact gttacgtccg
     8281 ctgcttgatg atgtgccgga ggatgaactg tgcggcgtag cgcccggaag agtgttgccg
     8341 gtatcagagc aatggcatcc actgttaatt gaggcgttaa ccagcattcc aaaactcgag
     8401 gccggagact ccgtctggtg gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat
     8461 caacagggtt ggggcaacgt gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt
     8521 gcctacgcgc acaaggtgaa ggccgcactg gaaaaaggcg catcgccggg cgacttcccg
     8581 cgcgaggact atgaaacaaa ctgggaagga cgctttacgc ttgccgacct caacattcac
     8641 ggtaagcgag cgttgggcat ggatgtttga gagttaatgg aagtgaattg atgtgggatt
     8701 aagaaaatcg attcgacgtt actcagattg ctgacaagtg cgctttctta atgcctgatg
     8761 cgctacgctt atctggccta caaagtcctg cagattcaat atattacagg tactgcgtag
     8821 atcctgataa gcgtagcgca tcaggctatt ttgcatttgt catcagccgc cgtcaccccg
     8881 acagtatcgg ggtgacggat aatcacttac tcgatccctt tactacgcag gtaatcttcg
     8941 taattaccgc taaagtcgat cacgcgttcc ggggtgattt ccagaatgcg ggtcgccagg
     9001 gagcttacga actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc
     9061 gccatgttca gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc
     9121 agaatgttcg gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg
     9181 gaaagcactt tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg
     9241 ctgcgtaccg cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg
     9301 gtcagatcat tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt
     9361 tcagaccatt ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc
     9421 gttgatttac cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc
     9481 aggttgagat ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc
     9541 agcgcgttac ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg
     9601 gaggctttca cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg
     9661 cgagatttcg aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc
     9721 gctttcttct tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg
     9781 tactcatcgt agttgcccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg
     9841 gtacaaacca tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc
     9901 tcgttcagca cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg
     9961 tcgagcagga gaatatccgg atcagcaaac agcgcctgcg ccagaagcac acgcagcttc
    10021 cagccaggag caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca
    10081 agcaacaatt caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact
    10141 tccagatcgg ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg
    10201 tcacgctcct gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca
    10261 gtgaactctt caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg
    10321 agggaaacgt tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat
    10381 ttaccactac cgttcgcgcc aatcaggccg taacggttgc cgccgccaaa tttgacggaa
    10441 atgttttcaa acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca
    10501 ggcgtatcct gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat
    10561 cctgctcctc gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca
    10621 tctgatttca ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc
    10681 aacccaatgg cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt
    10741 attcgcagcg gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc
    10801 tctgccaacc gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg
    10861 taacactcag ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat
    10921 ggaagcgaac ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc
    10981 gcagcagctg atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat
    11041 gcgtctgtat tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg
    11101 tcagttaggc aaagatacgc ctatcaactg gaccactaaa gttgagcgta agaaagcagg
    11161 cccgacctgg acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct
    11221 tccggctgtc gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg
    11281 tcgcctgtac gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca
    11341 tggttgtgtg cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg
    11401 tacccgcgta cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg
    11461 ttatattgaa gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat
    11521 tgtgccaatt accctgacga agagcgtgca aacagtgacc ggtcagccag atgttgacca
    11581 ggttgttctt gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc
    11641 ttcaaaactt aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag
    11701 ttggccggga gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt
    11761 attgaataga gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc
    11821 aaatttgcca gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt
    11881 aataaagacg gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc
    11941 tgcgataaac cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc
    12001 aacacgcctt gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata
    12061 aacaccagca gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag
    12121 cctgccgcca caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag
    12181 acgatataca gccccagaca actccacacc agccgcggtt tccactccgc tgtttgcacc
    12241 cccgtatggt attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc
    12301 agcatcattg cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca
    12361 gaacgtcccc agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg
    12421 ttgactgcca gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt
    12481 agcgtgatag tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa
    12541 agcagcgccg ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc
    12601 accattttgc gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc
    12661 atcaggccgc ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt
    12721 gcaaagggca cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga
    12781 tcgccttgca gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca
    12841 aacgaaaagc gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac
    12901 gggcgatttc cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct
    12961 cgcggctttc ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga
    13021 tcatttcaat cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg
    13081 aaactcccag acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt
    13141 cagaaatcag ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc
    13201 ggaacccttc aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg
    13261 tacctgcgcg acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat
    13321 ttcgtgcgca cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata
    13381 gctatataat atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac
    13441 gggattagca agtcaggagg tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt
    13501 cattctccct ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac
    13561 aaaccgcagc aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc
    13621 tgctttcatg caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc
    13681 aatccccttc gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc
    13741 ttcttgcagc cctgcgtata ttgcggctcg cggatgcgga cccctttcca ctcttcacgc
    13801 actcttgcag gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg
    13861 gcatatgaat ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg
    13921 gactgcgttc tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag
    13981 tttgctctac accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg
    14041 cgtacaaaaa gttctggtcg gtatcagttc gctggtggcg gcttgttatt tcccttttgc
    14101 tcaggcctac ggcgcgccta acttcaatac attgctggca ttgcactcca ccaatatgga
    14161 agagtcgacc gaaatcctga cgatttttcc gtggtacagc tacatggtcg gcatatttat
    14221 ttttgtgctc ggcgtaatag caatcaggcg aaaaaaagag agtgaaaaag cgcgctggaa
    14281 taccttcgac agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca
    14341 aaacctggcc tggggcggcg tatttaaact gaaagatact ggctatccgg tatttcgttt
    14401 tgctaaggat gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa
    14461 actttccgga atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta
    14521 tgtggtggtg atcggtgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg
    14581 ggacaatacc ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc
    14641 cagtggctcc acgcagaaat cgcttggctt aacgctcaat cgcgtagtcg atggcaaacc
    14701 acagtttcag gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt
    14761 ttccaaccag ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgagc
    14821 agatgaagtg tacttcctga aagaaggtaa ttttgaagca gataaaaata ccaaagacga
    14881 agcgttactg gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat
    14941 tgttctacat ctgatgggct cacatccgca ggcctgcgac aggacacaag gcaaatacga
    15001 aacctttgtg cagtcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga
    15061 tttactgcgc aagctgtacg atcagttacg caacagcggc agcagcttct cgctggttta
    15121 cttttctgac cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca
    15181 tgatgataag tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa
    15241 agcgcatcgg gtgattaaag cccgccgctc agccaatgac ttcttaggtt ttttctccca
    15301 gtggacggga attaaagcga aggaaattaa catcaaatac ccgtttatat ctgagaagaa
    15361 agccgggtcg atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg
    15421 aaccgatatt ttcgacccga agccttaaaa acaaaaatcc gccccgagag gcggattttt
    15481 tatatcacca aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa
    15541 cgctacgaat acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga
    15601 actgcagacc agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt
    15661 attcagtggt ctggaattta ccataaccca cacccactac accgtagatg cttgcccagt
    15721 cgttaatgcg gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac
    15781 cagagcttgc agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc
    15841 tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct
    15901 gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg
    15961 cggtgaaagc cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct
    16021 caaat
//
LOCUS       DAZFRN010000112        16005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFRN010000112 DAZFRN010000000
VERSION     DAZFRN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.114"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(434..913)
                     /locus_tag="ACHKUB_003467"
     CDS             complement(434..913)
                     /locus_tag="ACHKUB_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656439.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMLLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIMNSAASGSRD"
     gene            complement(1025..2269)
                     /locus_tag="ACHKUB_003468"
     CDS             complement(1025..2269)
                     /locus_tag="ACHKUB_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO6656440.1"
                     /translation="MTLPVDSLIAYTLEKMQERIVQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEDRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSDSEPSEKSEGYHRVRKPNHYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLERLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRSE"
     gene            complement(2287..2754)
                     /locus_tag="ACHKUB_003469"
     CDS             complement(2287..2754)
                     /locus_tag="ACHKUB_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656441.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            complement(2758..3249)
                     /locus_tag="ACHKUB_003470"
     CDS             complement(2758..3249)
                     /locus_tag="ACHKUB_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO6656442.1"
                     /translation="MQISPVTLRVAKAFISRHHRHNKPPVVHKFSIGLRNDAGELTGV
                     ATAGRPVARHFDDGLTLEVNRTCTTGERNANSALYGAVWRAAKDMGYRRCITYTRADE
                     SGASLRAAGFVRVKELPSGKSWAESSVALKSTRDPVGSGGVPRVLLEIRRMSTTVIHI
                     KGE"
     gene            complement(3252..3650)
                     /locus_tag="ACHKUB_003471"
     CDS             complement(3252..3650)
                     /locus_tag="ACHKUB_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656443.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VASCIIEDGGMCVDGGGEYVSHSLPGGTFQLYTASPVPVVPEGLVKAVRFYEQVKREN
                     PPVETGAWKDAVEWVLKEACQAVNIRIKGE"
     gene            complement(3654..3941)
                     /locus_tag="ACHKUB_003472"
     CDS             complement(3654..3941)
                     /locus_tag="ACHKUB_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656444.1"
                     /translation="MATLQELIDLTPEQEKAWKRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTARVYMPGIDAPGLTSWADDWHGITLKDGVEVDDD"
     gene            complement(4108..4716)
                     /locus_tag="ACHKUB_003473"
     CDS             complement(4108..4716)
                     /locus_tag="ACHKUB_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO6656445.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            complement(4713..5438)
                     /locus_tag="ACHKUB_003474"
     CDS             complement(4713..5438)
                     /locus_tag="ACHKUB_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO6656446.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            complement(5431..7164)
                     /locus_tag="ACHKUB_003475"
     CDS             complement(5431..7164)
                     /locus_tag="ACHKUB_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO6656447.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGVGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGDPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHYSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVSIFPGTECKQHGITRPLKAVSDADTECTEKCSRPSQEESAGRGDSGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDGAAGAVLASVMTGDTAPGTTDALPASVTH
                     FAVGRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            complement(7164..8531)
                     /gene="dnaB-PI"
                     /locus_tag="ACHKUB_003476"
     CDS             complement(7164..8531)
                     /gene="dnaB-PI"
                     /locus_tag="ACHKUB_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFO6656448.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            complement(8879..9781)
                     /locus_tag="ACHKUB_003477"
     CDS             complement(8879..9781)
                     /locus_tag="ACHKUB_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656449.1"
                     /translation="MSSSVKYSPVPAVLTRPESLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVERQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGSHPDVSVYAERCAQ
                     ETSLSPVHPTRQCPVQSTDSAETE"
     gene            complement(10017..10226)
                     /locus_tag="ACHKUB_003478"
     CDS             complement(10017..10226)
                     /locus_tag="ACHKUB_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656450.1"
                     /translation="MCVGCGYSGVRDGACKNAPVWVKVNPESGLGLCSGCDAFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
     gene            complement(10559..11446)
                     /locus_tag="ACHKUB_003479"
     CDS             complement(10559..11446)
                     /locus_tag="ACHKUB_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO6656451.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTMHSLACELLPEW
                     REFFDALLENGGKSLWAEN"
     gene            11762..12946
                     /locus_tag="ACHKUB_003480"
     CDS             11762..12946
                     /locus_tag="ACHKUB_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6656452.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHV
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            13275..14318
                     /locus_tag="ACHKUB_003481"
     CDS             13275..14318
                     /locus_tag="ACHKUB_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6656453.1"
                     /translation="MNDRLRFEVNDNQGCFVFPDAWFGSLLDEFDELIDAYDTDEISE
                     TSYINKLRRLARQEPDFIDVHAYLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAALLTDKILAYNPEDNQGARWLLGPEL
                     LRTGDHEQARHVLKAHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            14461..14690
                     /locus_tag="ACHKUB_003482"
                     /pseudo
     CDS             14461..14690
                     /locus_tag="ACHKUB_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14677..>16005)
                     /locus_tag="ACHKUB_003483"
     CDS             complement(14677..>16005)
                     /locus_tag="ACHKUB_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6656454.1"
                     /translation="TSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTDIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
BASE COUNT         3870 a         4601 c         3685 g         3849 t
ORIGIN      
        1 tttaatcact gttaccactg gcaacaccgc tcacttttta acctctgctg ccactggcaa
       61 caccgctcac tttttaatca ctgttgccac tggcagcact gctcattttt caatctctgt
      121 tgccactggc agcaccgctc attttttaat ctctgttgcc actggcaacg ccgcttattt
      181 tttaatcact gttgccactg gcaacatcgc tcatttttta acctctgttg ccactggcaa
      241 caccgctcac tttttaatca ctgttgccac tggcagcacc gctcattttt taatctctgt
      301 tgccactggc agcaccgctc attttttaat ctctgttgcc actggcaaca ccgctcactt
      361 tttaacctct gctgccactg gcaacaccgc tcacttttta acctctgctg ccactggcag
      421 cactgctcat tttttaatcc ctgctgccac tggcagcact gttcatgatg actactacct
      481 taacgttcag agacaaaaac agcattaatg tcgttatccc catcacgagc aactgaatgt
      541 cggttatatc cagatgagac aatgaccaga aaatcacaaa aaatgaaaag cattctgcca
      601 gcagactcag gaggagcata aattgcaggc cccctccgac tgtactgcct gtacgcgctt
      661 tataaagact gtatgtgacc cacagccagt tacttaacac caggaaaaac caccctgtgg
      721 cacatacgat ataacacata ctgttatcgt actgttcgtg cattgttttc agcgcagtga
      781 gcagggtctc tgtgcctgtc accagccccc agataaccac cactccggca atcacgcagg
      841 cacaaatttt agcgatcaac aggccacttt ctttcatggc tgcactcaga ctttcctgtt
      901 tgtatttctg catgctattt accaccttgt ttcacctgac cagttcccga ttctgttttc
      961 tttttaacca gtcgctggcg gcactggttg atttttgttc ccgcgaacat agcccgtatc
     1021 gcaatcactc agatctcatc gcagaacatc tgcgcagcga ggcccgtagt ccagccacaa
     1081 tgccactcag acgttctgct gaaatcggtt ccgtacgttt ccccgatgaa actgattgcg
     1141 cagtggctcg ccctctttct cctgccagta cggaggctgg cacaacaaca cgttgtttct
     1201 gcttcggcgg ataaaaattt ccctcccggg ctcgcttcag cactgcaagc aaccagccca
     1261 caggattctg cagcgagtgg ctgcccagca tccctgacag acgttccaga accccctgcg
     1321 ccagttcttc cggcagcgcc tgcaactgac tggtcagcat aatggcctcc tcctccctga
     1381 tttgccctgt cagcgcttca ggcaatacgt acgtgttttt aatcacactg tgtgtgatat
     1441 tacgtacgta atggttcggt ttccgaactc tgtgataacc ttctgatttt tctgatggtt
     1501 cggaatccga actcagtatt ttttgcccct gtttttcact gagttcggaa tccgaactca
     1561 gcattttcaa taccgaactc tgtgtttcta cccccggttc ggtttccgaa cccggctgca
     1621 ttctgtcctc agtcacccgc tgacgggccg ccatctgtcc cggtgtctgg gcactcccta
     1681 tccgggcttc cagctgactg atatggctgt gataatgacg cattcccgga tcatttttta
     1741 tttcgcacag aatatcccgg gccgtctgac ttatggtccg gttccggctc atacaggcat
     1801 ctgccaccat atccagataa cgtggatcca gtatttcagc atcccggcac gtcaccggtt
     1861 catcgtgctg ggcataaata ttgccccgta cccgcccttt ctcatcacgc acccgtttgc
     1921 acagactcag ccagcccgtg atccgcagca tcagcagcac ccggctgaca gtctccctgg
     1981 aggcttttcc tttccccggg gaagccagct gcagttgcag ttcgtcatat gtggggaaaa
     2041 ctgcgccttc attattctgt gcatacagcc gaatcatcat ccacgccatc ttatccagcg
     2101 gagaaagtcg agtatccaga agcagtcgac gcggaatggc atcatgcaca tttccggtaa
     2161 acagcagtcc gctttgtatt tttccgtcct gttgtcggcc tttctgcact atccgctctt
     2221 gcattttttc cagcgtgtac gcaatcaggc tgtctacggg taatgtcatt acacttttcc
     2281 tttttgttat acgtcagaca tccgtctgct ggtaacgggt atatcgccca tattcaccag
     2341 acagttacgc cggagatatg ccagcgcttc atcaggaacg gtacattctg gtctggcttt
     2401 tttcaggtta ctgattaatc gtttcatcag cattactggt tcatgtgcac ggatccaccg
     2461 tccccctgtt tgatcttcca gacagcgaat taacatctca gctggcaaaa gaaccccgtg
     2521 gttctcacac tcctttttct tctctgccgc agttaatttc tgttgcaatt caacaattgc
     2581 tgccagcgca gatttaatcg tttcactcgt cacccgacat ggataagcac gattcctgcc
     2641 ttctttcaga agcactgcat aaccatcaga gcggtagtga taacagtttg ctcccaacca
     2701 gatttcgtaa gtatcgccgc gcgaggagat acccgttctc tctgcaatag ccatatctca
     2761 ttcccctttg atgtgaatga cagtagtgct cattctcctg atttccagga gcacacgagg
     2821 aacaccaccg cttccgaccg gatcgcgtgt actcttcagg gcgacacttg attccgccca
     2881 gctttttcct gaaggaagtt ctttcacacg aacaaaacca gctgcccgaa gagaggcccc
     2941 ggattcatca gcccgggtgt atgtaatgca acggcgatag cccatatctt ttgccgccct
     3001 ccagacagcg ccataaagtg cgctgttagc gttacgttca ccagtagtac aggtacgatt
     3061 gacctccagt gtcagcccat cgtcaaagtg gcgtgcaaca ggtctgccgg ctgtcgccac
     3121 acctgtcaat tcacccgcat catttctcag accaatactg aatttatgca ccaccggagg
     3181 tttattgtgt cggtgatgtc tggatataaa cgccttcgca acacgaagag tgactggtga
     3241 aatctgcacg ctcactctcc tttgatgcga atatttacag cctggcaagc ctctttgagc
     3301 acccactcaa cggcatcttt ccatgcccca gtttcaactg gcggattctc acgctttacc
     3361 tgttcataaa agcgcactgc tttaaccagt ccttcaggca ctaccggcac aggtgaagcg
     3421 gtgtatagct ggaacgttcc accaggcagc gagtgactta catactcacc gcccccgtca
     3481 acacacatgc ccccatcttc aataatgcat gacgccacag gctctgcttc cagcgatgcc
     3541 agcgcgataa gcgccagctc acgaatctca ccgccgtcta tatcgtcaat gtcatcgcgg
     3601 ccagaaatgt tagccagcca ttgcagtcgc tctttggtaa tcgtggtcat gtgttagtca
     3661 tcatccactt caacgccatc tttcagcgtg atgccgtgcc agtcatcagc ccaactggtt
     3721 agccctggcg catcaatgcc aggcatatag actcttgcag tgtggtagcc cttatcgtta
     3781 tcaatgctgg caacgtgctc gccgttgtat gcactcagcg tgtccaggac gctataaaac
     3841 tttcctccgg ctgccctgaa atcctttaca gccttcacaa ggcgcttcca cgctttttcc
     3901 tgttctggcg tcaggtcgat tagttcctgc aaagtagcca tgttcactct cctttgatgc
     3961 gaatgtttac agcctggcaa tcctcctaga gcacccactc aacggcatct ttccacgctc
     4021 cagcctcaac cggctggttc tctcgcttca cctgttcagc ctcccgcagt acctgaaagt
     4081 taatttcggc catgtcattt gtccttgtca ttctgctttt tcccggttca gtttccggcg
     4141 actggccgat acatcattaa accccgcagt tccctgatgc catcccctca ctgcatgcca
     4201 taccgccgtc agcgggatat tcatttgctc agctgccagc atcatgacat ccagtccgtc
     4261 cgcactttcc acatctgtga tgccgctctt ctgccataaa cgccacagct ctgcactttc
     4321 ctcatcactc agtgtgatgc cacgtcccgg gcgaacatca ataccggcaa tgcgacggcg
     4381 cgctgccaca tccacactgg aaagtccaaa gtaaaacgcc atcagttcaa tggagcctcc
     4441 cagcgccagt gcccggtcaa tgcgctgcag acgtttctgc tccgtccggg cctgctgcag
     4501 catacgcagc aggttttcat gattaatgtc cagccggata accgacactt cagagtttga
     4561 caggtaatga atttcctcaa tggtcagtcc ctgcagcatc tgcatttcct cccgggccag
     4621 ccccagagat tcgcagcgac gcaggtagcc gctcttgagg tccatcacca gttgcatcag
     4681 cagaccgttg gttgcctgag acaagctgtt attcatgacg cctcccgtat catcagaccc
     4741 ggcaccacct gtcgggcact ctcccccaca ccatgatgca gtgaggcatt tttcccggcc
     4801 tcatacccct gccagcgggc caggtcaccg ccgcgacagg ctttcatctc acgggagtcc
     4861 accgtcacca tatcctgctg ctccatccag agacgcatca gccggctttc ctcttctgtc
     4921 acactgaatg gcatcaccac ctgacgaatc ccctgaaccc agccatcacg aaactgagcc
     4981 gcccgggcac gacgggtgct cagtttcagt ctccggctct gtgtacgaag atacgcatcc
     5041 gttgcttccc ttagctgacg gcacagaaca tcaaacgcat aagccgccac ctgaggacgt
     5101 tcactgaaac cgtaaaacgt cacacaccgg cgataccggc ctccgggcac cgcccgccag
     5161 tcgtaatacg cccggcaacc aaatgtctgt gtcaccacca tgaccagacg aaacatccac
     5221 gccggaactt tttcagcatc agaaggtgcc ccctgtgatg aagaggtctg caccgaagaa
     5281 agtccggcat cctgctccgt gataccgtgt ttcgccatca gctgttgcgc ccttgcaagc
     5341 gccagcccgg cctcatgggc attgctgtta ttacggctca gctccagcag tttcctgacc
     5401 cgcctgatca gacgttcctg atgcgacata tcactcatca ttctcatccc cctcatcgtc
     5461 gtcgggtaac acactgcgct gcagggctcg cagacggcgc agcacactca gaagacgcag
     5521 cagcttcacg gcatggtcgt catccagcag tggccacgct tccggtgcac tgccccctgt
     5581 aagcaggtct ttcagactca cggggaggac tgtccgcggt gcatcaccgg caagccccgc
     5641 cagaaacgcc actacaggag aacatttctc cactgctgcc ccgaaaccgg cagacaggtc
     5701 gctttccctg tccatggcaa tctcttcagc acagccggct gattcagcca gttcccaggc
     5761 cagacggaac gcttcattct gcagatgttc gatatcatcc cgccccacag caaagtgcgt
     5821 caccgacgcg ggaagagcat cagtggttcc cggggcagta tctcctgtca taactgatgc
     5881 cagtactgct ccggcagcac catcttccat aacatcacca ctgaagactg gcttaccacc
     5941 gtacatatcc ggctgtatct caggacgcag ggcattttca gcagaagacg ccccactgtc
     6001 ccccctgcct gcgctctctt cctgagaagg tcgactgcat ttttcagtgc actctgtgtc
     6061 agcatcagaa acggctttca gtggccgggt aataccatgt tgcttgcact cagtaccggg
     6121 gaatatggag actgtgcttt ccggcatcgc ctctgtatcc gtatccggtt gacactccgg
     6181 tacttcagac cggatcccgg ataccggtgc cccgggttgt accgtactgc tgccacggac
     6241 tgaaggaggc accaccaccg gttccggctc accaaaatga tgacgacggt tccgctcttt
     6301 cggatccagt tccagcaacc accggtcata gtccagggag ggatgtggca gggcctgcaa
     6361 caaatctccg ataagctcat cgcggaacat ctccagtgac cacagttccg gcgaattaaa
     6421 cttccggcag ctttccccga acacgcggtc aaattccgca tccgtatcgg ctgcaacagc
     6481 aaactgcccc catacacgtt ctgcgtcctg acgaagtgcg aggagtgctg taacctgtgg
     6541 acgccccagt cctgattcca tcaggtcggg gatccagggg tacagatact tcagggcatc
     6601 ttccattctg ctgattgtgg agtaatgtat cggcagccct tcatgggtta acaaatcagc
     6661 cagttcacga agagacactg ttctgcccag ttgttcctca tggatttgtc ttgccttgtg
     6721 gaccccctgc gccttctcaa taaaagtcag ttcaccgcga acttcatttt cagccagatg
     6781 accaatcaca cactggagcc gacccggcca gggtttaaac agcacatgaa tacggtaaaa
     6841 acgcggatcc cctgtttctt tccagagttc cgtcagtatc tggtaacggg tgtttccccc
     6901 gtcactgaag atatacacat ccggctcact gtccggcata cgggtgactt tgggcaccgt
     6961 atccagcccg cgggcatgaa tggaattttt gatatcgtca taacgcggat tgcgcgtggt
     7021 gcgcgggtta tccggattcg gacgtaactg gtccagcgtc agaaccatgg gcgtttcccc
     7081 cattgccacc acattgccga caccagtggt ctgacgaccg ggctgcataa tagccgcccc
     7141 gagatccagt ggtttatgtg ccatcacgca tatcctcccg gcccgtcatt ctcagagggc
     7201 gtaaacagtg tgtaacgacc gtcgaacctg acccgtaccg tacccgtggg cccctgacgc
     7261 tgcttgctga caatgatttc tgcctccccc tttgccagtg agtcggggtt atagacctcg
     7321 tcgcggtaaa taaacataat caggtcagca tcctgctcca gcgccccgga atcacgcagg
     7381 tcaccgttat tggggcgttt atcagcacgc tgctccacct gccggttaag ctgggagagg
     7441 gccagcaccg ggcagcccag ctccttcccc agcgctttca gggaacggga aatttcagca
     7501 atttcctgtg tgcggttttc cagctccggc gcacgaatca gttgcagata atccaccata
     7561 atcagggagg gtttaccgta cagacgtgta taacgacggg cgcctgcacg cagtgatgaa
     7621 ggcgtctgct cacagcagtc atcaattatt atgcgctcac ccagagagcc cattcctccg
     7681 tcaccaagcg ccataccgac cgcagatgac accctcagcc agtcctcatc atccatgttg
     7741 ccactgcgca aatgattcag ttccacacgc cccagcatgg aaagcatgcg cagaatcagt
     7801 tgctccttcg gcatctcaat gctgaatacc agaacgctct cttccgttcg ccgcagtgcg
     7861 gcgctgagac acatggataa cgccagagag gttttcccca ttgacggacg ggcagccagc
     7921 agaaccaggt caccaggctg caggccacag gtcatttcat ccagacagtc aaatcccgtg
     7981 ggggtaccgg taacaccacc ggagccgagc acactttcca gttgctggag aaccgtatca
     8041 agcgcggatg aaagagaggc ttccgtgcgc tcccggagcg tcctgttctg cgcaagcgca
     8101 aacagacgcc gctctgcagc atccagcaca tcccgtgatg tggcgttcgg tgccgccaca
     8161 tcagcaatca gggtgtttcc ggtggcctgt aactgacgca gaacgctttt ctccaccaca
     8221 atgcctgcat aggccatgat gttggccgca gaaggtgtgt tcttgctgag ctcggccaga
     8281 taagcaaaac cgcccagtac gtccagcctg ccctgatgtt ccaggtgctc tgacagtgta
     8341 atcaggtcca gtggctggcc attcaccgcc agttcagaca tggcacggaa aatagcccgg
     8401 tgctgagcca cataaaaatc gtcaggactc agcatcagca ccacttcatc ccagcgttca
     8461 ttctccagca tcagaccgcc cagaacggcc tgctctgctt cgtatgcgca gagaacagac
     8521 atttcagcca tggcgtaccc tccctctctg actgccggaa acctggctac gtgaaaaaag
     8581 ggaaaagggt gccggacgta cagttgtcgg gaataaaatc ccggtaactg tctgtacgtt
     8641 ttcagaccgg tgagtttgcc gaccccggcc tgaaggtacg gacagtattc gttcattcat
     8701 aacaagttgt tttaaaggaa aaagtttatg aatacagaaa aacaactgcc gtccggcatt
     8761 gaatctgaag tgaaatacac cctgcagttc tggctgctga gtaacggaaa ggtcatgacg
     8821 gaaaaagacg ggcgctgtaa cccctttccc ccgaactgta ttgaatcggc tcatcctttt
     8881 actccgtttc agcagaatca gtggattgaa ctggacactg tcgggtcgga tggaccggtg
     8941 aaagcgacgt ttcttgtgca cagcgttctg cataaactga aacgtcaggg tggctccctg
     9001 aatgatgccg gccggcctga atacggccgg tgcattgctc actggcacac tgacgtgttt
     9061 gttgagaaaa aagcgctgcc tgactggctg agagcattcc acactgacgt ttcgcactcg
     9121 taaaaacacg ttcagcccga cgggtcatat tttccagcca cccgtcgggg aaaatgtcag
     9181 caccatcacg gggaaacaaa atcgctttcc attcagggta tgagcgcgga atacctccgg
     9241 aagcattccg gctcagacag cgactcacag tgcaggcagc acggaatgca tatgttgcga
     9301 tgctctcatc atatgttgcg atgttctcat tattgccttt ctgccgttcc acccgctctt
     9361 cagcatcaaa tgccagtgca gcaaggcttg cccagcacat gcagttcact gaattccccg
     9421 gaacgctctg cagctcccgg cgcaccatgt tgacctcagc actgaaaaaa gcgttctcat
     9481 cgccttcaag ggcagccaga tgcagcggaa gccagacctg actgaaccat tcgcacatcc
     9541 gttcccggtt actgacggtc atcactgtct ccgggcggga accgtcctga aaaacatgtc
     9601 cggtgcgtgg agaactgtat gatacataac caccgtgcac cgcaatgacc ggacgacctg
     9661 acgccggaag ccccacacga cacccactgg caatccgggt catcagcagg ttcagttcag
     9721 ccagcgattc agggcgggtt aagaccgccg gaaccggaga atatttcact gaggaagaca
     9781 tactttcacc tccatatctc tcagcccgcc aggtgcgtca cttccccgca gataaccgga
     9841 aacctggcta tatgaaaaaa gggaaaaggg tgccggacga acagctgtcg ggaataaaat
     9901 cccggtaact gtctgtacgt tttcagaccg gtaagtttgg cgaccctggt cagaaggtac
     9961 ggacagcatc tttgcgctca taactgtacc gaccgggata tgcattacaa agactgtcat
    10021 accgccccct ccgtctttcg ctgtttgcag gaaagaccac atgaaaaaag ctggtcatgc
    10081 ctctgacgac gctggcgttc ctgcaaaaga aaggcgtcac aaccggaaca cagccccaga
    10141 ccactctcag gattcacctt cacccagacc ggagcattct tacaggcacc atcccgtacc
    10201 ccggaataac cacagcccac acacaccgtc cacttctgca gtacgatgag tgcagtttcg
    10261 agtgatgcgc ggagacgccg ggaggcaata aggagcaacg gctcatggtc ataaaaaacc
    10321 ccgccgttcc cccctccgga ttcaagcagc gggagtcctt caggctcccg gggacctgtt
    10381 gacatttccc gtaattcgtt ttcattccac atattgtgga aagttatccg gttcactgaa
    10441 caacgccatt cctctgccag acgctgatac aaatgctttt tcgcttctgc gcggtcagat
    10501 gcatcaacaa acacatgcac ttcctcgtgt ccgggtgctg aagccacggc acgccatatc
    10561 agttttctgc ccacaggctt ttccctccgt tttccagcag agcatcaaaa aactcacgcc
    10621 actccggcaa cagttcacag gccagactgt gcatggtgac cagcgcagaa tcgctgcaac
    10681 gggcagacac tttttccaga cgatgcaccg gctgaccggc tgcgtgcccc tgtttataaa
    10741 tattcaggtc gtaaatctgt gttgaaagaa gggttaccgc accttcaggc agcactttgc
    10801 tgtagcgacc ggcgttgata atgcctgtca gctctgccag tgtctgtctg gccagcgccg
    10861 tataatccat gcagttaacc agagtgcgga ctttcggcag gggaataccc agttcacggt
    10921 agggcataag gttttccatg acattgaccg tgccgcggat gaactcacgc acatccggca
    10981 gaaccggatt caccatcccc accacagact ccgtggcgga cagcacaatc atttccagca
    11041 tgacagaacg tgcgccctgt gaatccacca caatgacgtc ataggtctga aaaagcggat
    11101 gctgaagcac attacgcaga cgaaggcgac catccggcgc atgaagcatg gccgttttca
    11161 gttgctcatg ggggtcgttg gagacgatga ggtcaagccc cctgacagcc gtacgggaaa
    11221 tgatgttgtc aggatgactg aggtcagccg tgcgcatcag taattcaaaa agaccggccg
    11281 gcgcttcata cgtcagcgga aagatattgc ttgccgtggg ctgtgcatgg tctccgtcta
    11341 tcagcagagt acggaggccg gcatctgcca gaaaaccggc aagattagca gactgggttg
    11401 attttccttc gccaccttta gtggatataa ccggtaaaat tttcatatag cacactcttt
    11461 aaagtatgca accaatcact tacactacgg aaaccagcca gaatattcac ttcactacct
    11521 gaatactcgc aacacgggga ttgaaaatcc ccgtgtcctt ggttcgattc cgagtccggg
    11581 caccactttc acttcctgat tcatctcccg aactccacga aactcaagta taatcacatc
    11641 gttgcaagca atctgaacat tctgaactcc accagactct actgaactct ctctcaatca
    11701 agcgttcatt gggggccccg gtgggggctc attgtgttca gtcagtttca ggagccccca
    11761 tatggcactt acagccagac aggtcgaaac agccagacct aaagaaaaag actataaact
    11821 ctctgacgaa cgtggtttat atctgctggt aaaaaccacg ggagcccgct actggcggct
    11881 taaataccgg atagcaggaa aagagaaaaa actggccctc ggcgtctatc ccgacgtctc
    11941 ccttgctgag gccagaatca aacgcgacga tgcccgaaaa atcatctccg aaggtggtga
    12001 cccgggcgaa aagaagcgaa aggaaaaact cactcagaaa atctctgcca ccaatacgtt
    12061 ccatgtcctc gctacggaat ggcaccagca taaatctttg tcatggtctg aaagttacgc
    12121 cagaagcgta ctggaagcgc tggataaaga tattttcccg tatctgggca aacgaagcgt
    12181 tacggatatc ctcccgctgg aaatgctgga aattctgcgc cgcatagaaa aacgtggctc
    12241 gctggaaaaa ctgcgtaagg tccgtcaata ctgtaatcag atttttcgtt atgccatcgc
    12301 caccggacga gccactgtca atccggcatc tgaactgacc agtacactgg cggcgccaaa
    12361 agcggcgcat ttcccccacc tgagagcaga tgagctccct gtttttctca ggaagctcgc
    12421 tgaatatcat ggcagtccag ttacccgcat ggcgacaaac ctgctgcttc tgactggcct
    12481 cagaaccatt gaactacggt ctgctgaatg gtcagaaatt gattttgata atgccctgtg
    12541 gacaatccct gaaagccgca tgaaaatgcg acgtaaacat gtcgtaccac tgtcacgaca
    12601 ggtcactgac attctgcaac aactcaaaac tttctccgga caataccggc tggttttccc
    12661 gggacgttgt gatatcaaca agccaatgag cgaagccagc atcaatatgg tgctcaaacg
    12721 tatcggttac gatggcagag caaccggtca tggttttcgt cacaccatga gtaccattct
    12781 gcacgaacag gggtttaatt ctgcctggat tgaaatgcag ttagctcatg tggataaaaa
    12841 ttccatcagg ggtacctata atcatgccct gtatattgat ggtcgccgtg aaatgatgca
    12901 gtggtatgct gactacattg attcgctttc tgagcaggta agttaagctg agaacacgtt
    12961 attctgattt tcacttactt cacgattatt cctccggcct gcaggttcgg aggattctta
    13021 tttttatgtt ttattttcag ataaagtttt tgacaaaacg tttattctgc atcagcaaaa
    13081 ttatcacaaa acaacgcaat cacagacaaa ttaacatttt tcagacgggt atattgagtg
    13141 cccacaatgt aagacaggca ggcatccggt gacatggtaa aatattaatc cagggcaaaa
    13201 acaaggcttg atacacaaca tcatggcttt tatgctctca gactatgcca ccacagattt
    13261 tacaggctca gaaaatgaat gacagattac gctttgaagt gaacgataac cagggctgtt
    13321 tcgtgttccc ggacgcctgg tttggctcgt tgctggatga gtttgacgaa ctaattgatg
    13381 catacgacac agatgaaatc agcgaaacaa gttatatcaa caaactcaga cgccttgccc
    13441 gacaggagcc tgactttatt gatgtacacg cttacctggc atacgcattt ctggagcaga
    13501 atgccccgcg taaggcactg aacgctgctc ttaaaggact ggcagcaggt aatcgtctca
    13561 tcccggaaag tttcagtggt gaaatcatct ggatgcatcc cgaaaaccgg ccttatctgc
    13621 gcgcgcttta tgcagcaatt ctcgcaaatg tgcacttaca gcgtcatcag gatgcagctc
    13681 tgctcactga taaaatactg gcatacaatc ctgaagacaa tcagggagca cgctggctac
    13741 tgggaccgga actgttacgt actggcgacc atgaacaggc acgccatgta ctgaaagcgc
    13801 atgcagatga attttcacct tactggtatg aactcggcct tctgcatttt cttaatgggg
    13861 agctggtaaa agctgcaact gcatttcgtc gcggctttgc ggcaaatact tacatcgcag
    13921 aaatactgtg cggaaatctg catccatttc cacttgctgt atggcataac ttttcaggcg
    13981 gtccggatac tgctgaagat tattatgcca cataccaccc gttatggggg caatatccgg
    14041 aagcattgct gtttgtaaac tggctctata atcattcctc tgtgctgcat gaacgggcag
    14101 aaatcattaa atgcgccgaa atgctgatgc aggaagatga tttcgaaata tgcgaaagta
    14161 ttttaagaca gcaggagaag ttgcgtgaaa gaattgatga gacgctttct gagaaaattg
    14221 tacagaaatg cagaaatatg aatggtgaat atgtctggcc ctggatattg ccgttttcag
    14281 cggcaggcat gaaacatact ggcatacagt atcagtagat attgcattag tgtatcctgc
    14341 acacaagtaa taatttatcc accaataata acactgttaa tgtccccttc ccctggttgt
    14401 cagccagggg ttatcttctg aatatttctt ttgaaaagga taacacagta aattattttt
    14461 atgaattatc ccatggactc attaacaccc tttcataatg ctttattgtc aaacacgtta
    14521 tggctgacat caaaaaaacc ggatttcctc tgccagcggg taatcacctc cccggtgttt
    14581 tcggttggtc tggttactcc agtctggtaa ttagcaagat aatcgatata aacagtggaa
    14641 aactcatcgt acataatctg gtgatgaaca ttacgcttat tttcccctga ccggaagaat
    14701 cagaggctgc ggtttcagac tgtctgccgg tacattcctc tccccgttaa aaaccataac
    14761 gggttcatta tcttcgtctg tcagcggatt gaatggcggt atattttcag tacgaatgtc
    14821 ggtcagccac tgaaaaatac ctgcgaaatg acgggcactg attttcctgc tgacggactg
    14881 atgagacgtg atgtcactgg cggtaataat caggggaacg ctgtagcctc cctgcacatg
    14941 accatcatga tgaacaggat tggcactatc gctgaccgac agcccatggt cagaaaagta
    15001 aagcatggca aaatgacggg aatgccggcg aaggatacca tcaagctgac cgagaaagtt
    15061 atccagttta ctgatgctgg cgaggtaaca ggcaatcttt cggggatact gctccaggta
    15121 atgattcggc caggagttaa gtcggtcaca cggattcgga tgagagccaa tcatgtgcag
    15181 gaaaatcacc ttcggagagg atttatccgc cagcgcacgt tctgtttcct gtaacaacaa
    15241 catgtcatcc gttttacggg aagcgaagct gcctttcttg aggaaaacgg tatgctccgc
    15301 atcagaagca ataacagaga tgcgtgtgtc atgctctccc agttttccct gattggatat
    15361 ccaccatgtg ctgtatcctg cttttgctgc cagcgccacc acgttgttgc cggaatcagg
    15421 gttctgctca tagtcataaa tcagtgtccg gctcagggaa ggtacggtac tggctgctgc
    15481 cgatgtatag ccgtcaataa ataaaccggg agccgtattc agccacggtg tggttggtac
    15541 gggataacca tataccgaca tataatccct gcgcacactc tcaccagtga caatcacaat
    15601 cgtgtcatac aacggtgctc ccggcaggat tttccagctg tcagccctgt gctgactcag
    15661 ttgttcataa cgctgcattt cacgcaatgt gtcagttgtc cccacaacgg ttcctttaac
    15721 catccgcaac ggccagctgt ttacagagca taatacgaac agcagcagtg ccagccagtt
    15781 acggtgaccg cggtggtgtg tccgccagaa aataaccatg aacaccagaa tcgcggcact
    15841 gaccagaaaa tgataaacag gaatcatccc ggtaaactcc gcagcctcat ccgccgtggt
    15901 ctgcaacaac gcgacaataa aactgttgtt gattttaccg tacgtcatac ccgcaggcgc
    15961 atacagtgca caacagaaca gaaataacag agctgtaatg gatgt
//
LOCUS       DAZFRN010000113        15759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFRN010000113 DAZFRN010000000
VERSION     DAZFRN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.115"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..677)
                     /locus_tag="ACHKUB_003484"
     CDS             complement(<1..677)
                     /locus_tag="ACHKUB_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6656455.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACCFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRRVKFS"
     gene            685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKUB_003485"
                     /pseudo
     CDS             685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKUB_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1132..1320)
                     /locus_tag="ACHKUB_003486"
     CDS             complement(1132..1320)
                     /locus_tag="ACHKUB_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO6656456.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(1324..1683)
                     /gene="yeaR"
                     /locus_tag="ACHKUB_003487"
     CDS             complement(1324..1683)
                     /gene="yeaR"
                     /locus_tag="ACHKUB_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO6656457.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1856..2494)
                     /gene="leuE"
                     /locus_tag="ACHKUB_003488"
     CDS             complement(1856..2494)
                     /gene="leuE"
                     /locus_tag="ACHKUB_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO6656458.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2621..3544)
                     /gene="dmlR"
                     /locus_tag="ACHKUB_003489"
     CDS             complement(2621..3544)
                     /gene="dmlR"
                     /locus_tag="ACHKUB_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO6656459.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3647..4732
                     /gene="dmlA"
                     /locus_tag="ACHKUB_003490"
     CDS             3647..4732
                     /gene="dmlA"
                     /locus_tag="ACHKUB_003490"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO6656460.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4983..6593
                     /gene="yeaV"
                     /locus_tag="ACHKUB_003491"
     CDS             4983..6593
                     /gene="yeaV"
                     /locus_tag="ACHKUB_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO6656461.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6625..7749
                     /gene="yeaW"
                     /locus_tag="ACHKUB_003492"
     CDS             6625..7749
                     /gene="yeaW"
                     /locus_tag="ACHKUB_003492"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO6656462.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGGGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7805..8770
                     /gene="yeaX"
                     /locus_tag="ACHKUB_003493"
     CDS             7805..8770
                     /gene="yeaX"
                     /locus_tag="ACHKUB_003493"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO6656463.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLKENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8824..9939)
                     /gene="rnd"
                     /locus_tag="ACHKUB_003494"
     CDS             complement(8824..9939)
                     /gene="rnd"
                     /locus_tag="ACHKUB_003494"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO6656464.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10021..11706)
                     /gene="fadD"
                     /locus_tag="ACHKUB_003495"
     CDS             complement(10021..11706)
                     /gene="fadD"
                     /locus_tag="ACHKUB_003495"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO6656465.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11911..12492)
                     /gene="yeaY"
                     /locus_tag="ACHKUB_003496"
     CDS             complement(11911..12492)
                     /gene="yeaY"
                     /locus_tag="ACHKUB_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO6656466.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(12532..13227)
                     /gene="tsaB"
                     /locus_tag="ACHKUB_003497"
     CDS             complement(12532..13227)
                     /gene="tsaB"
                     /locus_tag="ACHKUB_003497"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO6656467.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(13285..15195)
                     /gene="yoaA"
                     /locus_tag="ACHKUB_003498"
     CDS             complement(13285..15195)
                     /gene="yoaA"
                     /locus_tag="ACHKUB_003498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO6656468.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            15327..15671
                     /gene="yoaB"
                     /locus_tag="ACHKUB_003499"
     CDS             15327..15671
                     /gene="yoaB"
                     /locus_tag="ACHKUB_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6656469.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3826 a         4148 c         3888 g         3897 t
ORIGIN      
        1 ttgctgaatt tgaccctgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg cccaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgat cattgatgct gaagggtgaa ccggagttga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgaccgtgac atttttcaca
      241 acacccgtga cctgacggct attccggtag cgcatccagc ccagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccgc ttccggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acaacatgcg
      601 cctgcgaagc gcctcatttc acactcctgt tgaccacctg gtcttaactg gaatttaaat
      661 gcctgtaatc gtttcacagg tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccagatac
      781 cgactccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga acttgttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctagcta ttttgccagt agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cggataagaa cgcacggtat tcaccagatc
     1201 ttttatcact tcagccgcca cttctggcac cagcaaagtc atcggcgtct ctgtttcata
     1261 atcgacagaa acgccattgc tgttattggt gacggtcacg gtatacgttg ctttgcccat
     1321 gattcatttc ccgttatgaa tgactttccg ttgttgcgca ccttccatca ggacttcagg
     1381 agccacgaag aagtcaatgt tgaaataagt atcgtcagtc atggcttcaa tgttgtgcca
     1441 cttttctgga ggaaacaccg caaactgccc cgcttcgata aggatcacct gatcaggctc
     1501 tgcactgtgt tcatcagcgt agccgagata tttgaccgcc ccatgcataa cggaaaggcg
     1561 tgggtaaacc cccgggcgcg ttcctttatc aagatgacgt tcgaatattc cggcaggcgc
     1621 agtttgttta ttccagaaag gcgttgagcg cgtatgaata taattctgtg ggatttgcag
     1681 catcctattc cctccttcgg ggaatgcgct gaaaacggtt tattccagcc gtttcagggt
     1741 acgcctgata atttgcattt taaataccat ttattggtta ctttttagca ccatatcaga
     1801 gaaaaatcag ggaggattac aagtggggag cccgcgacaa cgcgggctga atgtatcagg
     1861 attgcagcgt cgccagtcgg gcggcgaaac ccacgaacat caaaccaatc agtgagttgc
     1921 caactttagc cagtttcttt ttggtacgta tgtactgcgt gacaaaagca ccagaaataa
     1981 tcaggaagct caaatagcag aaactcacca gttccagcgt cgtcgccaga ataaagaatg
     2041 aaattcccgt atgtggggca ttaacatcga taaactgtac gaaaaacgac acatagaaca
     2101 aaatggcttt cggattagtc aggctcaaaa ttaacgcgcg tttaaaaatg gccccgtatt
     2161 ggggctcatc ggatttggcc tcgttatttt tacccttcag ggtcgcgtag agaattttac
     2221 tccccagata gagcaaataa aacgcaccaa gataacgtac aatgttgaat aatatcgggg
     2281 tggtcttaat taatgtcgcc actccagccc atgccagaaa catcaatact gcatcgccaa
     2341 taaatacacc gcaggccgca agataaccgc ctttcatacc gctactgacg ctatttttga
     2401 gtacaaacag ggtatttggc cctggcacca acacaataaa aatggcccca accagatagg
     2461 tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt atctttcgat
     2521 catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa ggagaaaatc
     2581 aggttttaac ccgatatcaa cccgataatt gaatcattaa tcaggcatgc tccagtgaaa
     2641 aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc cgcactttcg
     2701 ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg taatattccg
     2761 gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac caggagcgca
     2821 gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga ttagacgaca
     2881 gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc cagacaccaa
     2941 aaggatgatc gcgctcttta atgaccaggc acggcaatgc agaaagatcg gttaaatgtt
     3001 tcggcgcacc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg taattagtcg
     3061 ccagtttgcg ggcgattaaa ttaggtgcga tgtcatcgcc aatgcgaata tcgagatcaa
     3121 caccttcatt gaccaaatcg accaaccggt cttccacatc aaaacgtaat tccagttgcg
     3181 gatacgcctt cgccagcgct gataacgccg gagccaccac ctgtcgtcca aaaccaaagc
     3241 tgctgataat acgcagcatc ccctggggca cctgacgcac gtcagaaagt tcgtccatca
     3301 tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct ccttcttcgg
     3361 taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt tgctccagca
     3421 aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc acggcggcaa
     3481 aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc ggtaaattat
     3541 tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc agtcaattat
     3601 aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga tgaaaacgat
     3661 gcgtattgct gcgatcccgg gagacgggat tggcaaagaa gtccttcctg aagggattcg
     3721 cgtgttacag gctgccgctg agcgctgggg cttcgacttg agttttgagc aaatggagtg
     3781 ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact ggcatgagca
     3841 acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggata ccgttccgga
     3901 ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc agtacgtcaa
     3961 cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa aacagcctgg
     4021 cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct cgctcggcgg
     4081 tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat ttacgcgtcg
     4141 tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc cacgtaagac
     4201 gctaacttct gccactaaat caaacggttt agccatcagc atgccgtact gggatgagcg
     4261 agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc atattgatat
     4321 tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg cgtccaattt
     4381 atttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg gcattgcccc
     4441 atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagccag tccacggttc
     4501 cgcgccggat atctacggga aaaacattgc taaccctatc gccacgattt gggccggtgc
     4561 aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc ataacggtat
     4621 tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata tgaaaggcaa
     4681 tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt aaggtcaaac
     4741 cagtttattt gaaccgcgtc actgacgcgg tttttttatt cgttctttgc agtaaataac
     4801 ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt atggctttcc gtattcttaa
     4861 ttgtttaatt tatgtaacat gcaaataatc aatttgttaa acacaacata caactcatta
     4921 ccctgcaaat aattacaact ggcaaaagcc agagacgggc ttttatgccc aggagatgac
     4981 tcatgatgag caatgttaag aaaaaagatg tgccgctgat aagcatcagc ctggtggcca
     5041 ttcttttcat cgcagctgca ttaagccttt tcccacaaca atcggccgac gcggccaacg
     5101 caatatacac ttttgttacg cgtacgttag gttccgccgt acaggtattg gttttgctgg
     5161 caatgggact ggtgatttat ttagccacca gtaaatacgg caatattcgt cttggcgaag
     5221 gaaaaccgga atacagcacg ctctcctggc tgtttatgtt tatttgtgcc ggtttaggtt
     5281 cttctacgct ttattggggg gttgctgaat gggcctatta ttatcaaacg cctggattaa
     5341 atatcgcacc gcgttcacaa caggcactcg aatttagcgt cccttactcc ttcttccact
     5401 ggggcatcag cgcctgggca acttatacgc tggcctcatt aatcatggct tatcactttc
     5461 atgtgcgtaa aaacaaaggt ctgagtcttt ccggcattat tgccgcgatt accggcgttc
     5521 gcccgcaagg cccatgggga aaactggttg atttgatgtt cctgatcgcc actgtcggcg
     5581 cactgactat ttcccttgtt gtcaccgcag caacctttac tcgtggactt tccgcgctga
     5641 ccggtttacc cgataacttc accgtgcagg catttgtgat cctgctttcc ggcggcattt
     5701 tttgcctaag ctcgtggatt ggtatcaaca acggtttgca acgtctgagc aaaatggttg
     5761 gctggggcgc gttcctgctg ccattactgg tgctgattgt cggcccaacc gaatttatta
     5821 ccaacagcat catcaatgcc atcggtctga ccacgcaaaa cttcctgcaa atgagcttat
     5881 tcaccgatcc gcttggcgat ggttcattta cccgcaactg gaccgttttc tactggctgt
     5941 ggtggatctc atacacccct ggcgtagcaa tgtttgtcac ccgcgtttcc cgcggtcgta
     6001 agattaaaga agttatctgg ggactgatcc tcggcagcac cgtcggttgc tggttcttct
     6061 tcggcgtaat ggaaagctat gccattcatc agtttatcaa tggcgtaatc aacgtcccac
     6121 aggtgctgga aacactgggc ggcgaaacag ctgtacagca agttctgatg tcgttgccag
     6181 ccggtaaatt gttcctcgcc gcatacctgg gcgtgatgat tattttcctt gcctcgcata
     6241 tggatgcggt ggcctacacc atggctgcga ccagtacgcg taatctccag gaaggtgacg
     6301 atcctgaccg tgggctgcgt cttttctggt gcgtggtgat cactctgatc ccgctttcca
     6361 tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac cgtcgtgctc acagcccttc
     6421 ccttcctcgt cattttactg gtgaaagtcg gcggatttat tcgctggctg aaacaggatt
     6481 acgccgacat tccggctcat caagttgaac attatctccc gcagacaccg gttgaagccc
     6541 tggaaaaaac accagtgctc cctgcgggaa ccgtattcaa aggcgacaac tgagcgccat
     6601 catcctaacg ataaaggtat ccctatgagc aatctgagcc ctgactttgt actacccgaa
     6661 aatttttgcg ctaacccgca agaggcgtgg accattcctg cccgttttta taccgatcag
     6721 aacgcgtttg aacacgaaaa agagaacgtc ttcgccaaaa gctggatttg cgtcgctcac
     6781 agcagcgaac tggcgaatgc caatgattat gtgacgcgtg agatcattgg cgaaagcatc
     6841 gtgctggtac gcggtcgtga taaggttttg cgcgcgttct ataacgtgtg tccgcaccgt
     6901 ggtcatcagt tgttgagcgg tggaggaaaa gcaaaaaatg tgattacctg cccgtatcac
     6961 gcatgggcat tcaaactcga tggcaatctg gcccatgcac gtaactgcga aaacgtcgcc
     7021 aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc tggaagaata tgccggattc
     7081 gtcttcatca acatggaccc caacgccacc agcgtagaag atcagttacc cggtctgggc
     7141 gcgaaagtgc tggaagcctg cccggaagtc cacgatctga aactggcggc ccgctttacc
     7201 acccgcacgc ctgccaactg gaagaacatt gtcgataact atctcgagtg ctatcactgt
     7261 ggtccggcgc atccaggttt ctccgactcc gtacaggttg atcgttactg gcacaccatg
     7321 cacggtaact ggacgctgca atacggtttc gccaaaccgt ccgaacagtc gtttaaattt
     7381 gaagagggta cggatgcggc attccacggt ttctggctgt ggccgtgcac gatgctgaac
     7441 gtcaccccga tcaaagggat gatgacggtc atttatgaat tcccggtgga ttctgaaact
     7501 accctgcaaa actacgatat ttacttcacc aatgaagagt taaccgacga gcaaaaatcg
     7561 ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt tacgtctggt tgaaagcgta
     7621 cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc gcatcatggc cgacagtagc
     7681 ggtagtggca tttccgaaca tggtatcgcc catttccata atctgctggc gcaggtgttt
     7741 aaggactaat gacatcggcg gcggtatttt ccgccgctgg gctgattttt gatggagtac
     7801 agcaatgtca gactatcaaa tgtttgaagt acaggtgagc caggttgaac cccttaccga
     7861 acaggtgaaa cgcttcacgc tggtggcaac cgatggcaaa ccattacctg cgtttactgg
     7921 aggaagtcac gtcattgtgc agatgagcga tggtgataac cagtacagca atgcgtattc
     7981 actactgagt tcgccgcatg acacctcttg ttatcagatt gccgttcggc tgaaggaaaa
     8041 ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa gtgggcaatc ggttaacgat
     8101 ttcaacgcct aataacctgt ttgcgctaat tccctcagcc agaaagcatc tgtttatcgc
     8161 gggcggtatt ggtatcaccc ctttcctgtc gcacatggca gagctgcaac acagcgacgt
     8221 cgactggcag ctacattact gctcgcgaaa tccagaaagt tgcgcatttc gtgatgagct
     8281 agtccagcat ccgcaggctg agaaagttca cttgcatcac tcatcaaccg gaacacgact
     8341 ggaattagcg cgattattgg cggatatcga acctggcaca cacgtttata cctgtggtcc
     8401 cgaggcgcta aatgaagcgg taagaagtga agctgcgcgt ttggacatcg ccgccgatac
     8461 gctgcacttt gagcaatttg ctatcgaaga caaaaccggc gatgccttta ccctggtgct
     8521 tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg actattttgc aggttattga
     8581 aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg gaacctgcga
     8641 aacagcaata ctggaaggtg aagctgacca tcgggatcaa tattttagcg atgaagagcg
     8701 tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac gcctggtgtt
     8761 ggatttgtag tttgccaaag ccggatgtgg cgctgagcgc gcccagtccg gcttcggaag
     8821 attttactgc ggatattcct gcaataaatt gtgtaatgct tccgccatca gctcaccacg
     8881 ccagccggaa atcagctccg gcaaattgtt ctgcggtttc agtttccagt gccagttcag
     8941 cagttggttg atttgccgac gcgatgccag caattcggcg ctgatcttat gcgtttcgct
     9001 cacgtcagta atcagcgact taatcgcttt aaacgcttta cgataaccag gcatgtccat
     9061 caggttaagc atcggctgcg gtaaggcctc ttccggcaat gtctgcgctt tttccaccag
     9121 cgccagcagc gttttaccgt gaaagcggat ttcgctaccg gataaaccca ggctgtccag
     9181 ttcgcctaaa cttcccggca tataacgcgc gaccgaccac aaatgctctt cacgcacgac
     9241 aaagttcacc gccagatcgc gctctcgcgc cttgcgcagt cgccagtcgg ctaacagttg
     9301 cagacaggcc agttggcgtg tgcgtaattg ccaggcattg gtgatatcac gccaggcatc
     9361 ttccggcgca acgacttcct gacgacgcat ttgcatcagg cggcattcat ccagcgccgc
     9421 aggtagccag ccggaggcct ccgtttctac cataagcttg gcggtgatcg gtaacagata
     9481 ccagacatcc gccgctgcgt attcacactg acgttcggtc agcggtctgg ccagccagtc
     9541 ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac tcttccacca tggaagcgaa
     9601 accccatgac atcgggcgtc cgcagaaggc agcaaggatt tgcgtgtcaa tcaagggttg
     9661 tggtaattcg ccaaagacat tgaggaacac ttccagatct tcactgcctg catggagaaa
     9721 ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg tgatcccgag
     9781 aggatcaatt agcgccagat gctcgccatc gaaaagttga atcaacccca gctgcgggta
     9841 ataagtgcgc gtacgaacaa attcagtatc cagggctatc gccggaaagg cacggacggc
     9901 ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt tggtaattca aattgttttc
     9961 tctttagtgg gcgtcaaaaa aaacgccgga ttaaccggcg tctgacgact gacttaacac
    10021 tcaggcttta ttgtccactt tgccgcgcgc ttcgtcacgt aattctcgtc gcaaaatttt
    10081 tccgacgtta gatttcggta actcatcacg aaactccacc agcttcggta ctttgtagcc
    10141 cgtgagctga cggcggcaaa aggtcaccag tgactcttcg gtaagcgatg gatctttttt
    10201 cactacgaag attttcaccg cttcaccact ggagccggaa ggtacgccaa cagccgcgac
    10261 ttcctgtacg ccaggatgct gcatgacgac atcttcaatc tcgttgggat agacgttaaa
    10321 accggaaacc agaatcatgt cttttttacg atcaacaatg cgcaggaatc cttcttcatc
    10381 cattaccgcg atgtcgccgg tgtgtaacca gccatttttg atgatttcat cggtagcatc
    10441 gggacgctgc cagtaaccca gcatcacctg cggtcctttg acacaaagct cacccggttg
    10501 acctggtggt acttcattat catcatcatc caccagtttg gcttccgtcg acggcaccgg
    10561 caaaccgatg ctaccactat gataatcaat atcatatggg ttaacgctga ccagcggcgc
    10621 acactcggta aggccatagc cttccagcag atactgtccg gtcagtttca cccaacgctc
    10681 tgccaccact tgctgcactg gcatccctcc gcctgcggaa agatgcagac tggagaaatc
    10741 cagctgctgg aactctttat tgttcagcaa cgcattgaac aaggtgttaa cgcccgtgat
    10801 agcggtaaac ggatatttcg ctaactcttt taccaaccct ggaatatcgc gcgggttagt
    10861 gataagcagg ttctgcccac ccagttcgat aaacagcagg cagttaatgg tcagggcaaa
    10921 aatgtgatac agcggcagtg ccgttactac cagttcttta cctggatgca gcaacggccc
    10981 ataggtggcg ttgacctgtt ccaggttcgc cagcatattg cggtgagtca gcatcgcgcc
    11041 tttcgccaca cctgtggtgc cgccggtgta ttgcaggaag gctaaatctt ccggcaccag
    11101 ttcgggtttg acgtactgca tccggtagcc gttatgcagt gcgctacgaa atgaaatggc
    11161 atctggcaga tggtatttcg gcaccaaacg cttgatgtat ttaacaacga aattgactac
    11221 cgtgcctttt gccgtagata gctgatcgcc catacgggtc agaattacgt gctgaacggc
    11281 ggttttatca accacttttt ccagtgtgtg agcaaagtta gacacgataa caatcgccga
    11341 tgcgccgcta tcgttaagct gatgctcaag ctcacgcggg gtatacaacg ggttaacgtt
    11401 tacgacgatc atcccggcac gcaaaatgcc aaacagcgcc accggatatt gcaataaatt
    11461 aggcatcatc aacgcaacgc gatcgccttt cttcagcccc aacccttgtt gcaaataagc
    11521 ggcaaacgcg cgactgcgtt cttccagctt gcggaaggtc attacctccc ccatattcac
    11581 aaacgcaggc tgatcggcgt agcgcgcgac cgactgctca aacatatcta ccagagattg
    11641 ataacggtca gggttgatct ccgtcggaac gtccgcggga taacggttaa gccaaacctt
    11701 cttcaattct tcacctctaa aatgcgtgtt cgtcgtcatc gcaaccccaa atgatataca
    11761 tgccgttaac ataatattaa ctcatcatac cagcttgata attacccaac gaaaaggttg
    11821 cgaagcgcgt cactatttat ttttatcttt accgtaagaa tgcagaaaca gcggaccagc
    11881 cgctgtttct ttttctttaa aaacaagcaa ttattcagtt acaactgttt gtactctcgc
    11941 ggggccggga ttataccagc cccatccgcc gtagccatag ggccagccac gaccgccata
    12001 aaaccatgga tcaatcggct gaggcggcat aatcacctgc tgggttaaat gccaacgttt
    12061 gtaacccgtt acttgcatca ccataaattt atagggcgtg ttgccgattt ttccgtcaac
    12121 cgcaccagtg attggcccga ctaccgtaac cagttgtcca cggaaatcca ccgggtccag
    12181 aaaaccgttc acatcggcat aaatgcgacc gcgagaaggt tctcccagcg tcggtctggc
    12241 accgctgtcc agcggtacgg tagcaatttc caggcgggtt ttcccttgct ggttttgtac
    12301 cgcaaccact ttgccaccaa agcgtgcctc ctgaccaacg tacagctgcg gcgcactcat
    12361 cacccgaact aaatcttgtt gcggcgtggg actgctgcct ttaatggcgt ccggcacagt
    12421 gacacaaccg ctcagcatta gcgcaaacgt acctgccagt atgcctttga taacattctt
    12481 ttgaaccgcc atgatgcgac tccttttttt ctcagggcat actcttaaga ttcattcttt
    12541 gcccggaagt ttcttccatg cgacgttgtt acgtaaataa accggttcgg catgttccac
    12601 cgccaccgtt ttaccctcgg caaacatctg acaagcaatc ggcagcatat cttctgcagc
    12661 ggggagtaac acttcgccat cgcgcaaaac cagtccgctc tctttaccga gatccggcca
    12721 ggcttgccag cctgtaccta ccgtcaccca ttccccggaa agctgttgca ttcgttcatg
    12781 aacgagttcg ggtttgagta cggcttcggt ttcttcaccg tgccagatac cgttttcatc
    12841 acgctgatat tcggcccagt aaacttcgcc cattcgcgcg tcaatggctg acagcacgcg
    12901 tgttgcgccg tttttgcgcc acgccccttg cgccatcgtc attagcgtgg agacgccaat
    12961 catcggtaat tccgcgccaa gcgccagccc ttgcgcgatg ccaataccaa tgcgcacgcc
    13021 tgtaaagcta ccggggccgc gcccgtaagc cagagcgtta atatcagtca gggaagttcc
    13081 gctggtggtc aggatatcct gcaccatcgg taagattcgt tgagtatgtt cacgagggca
    13141 aagctcaaaa tgagcgttga cagtaccgtc gttccacagg gcgacagagc aggcctctgt
    13201 cgcggtatcg atagccagaa ttcgcatggg tcttcgtgct tagatcaata aaaaggcgcg
    13261 catcatacca tactccgtaa caaattacct ggaggatggt atcgcaagga aacgaaccgc
    13321 acgggcaatg tcacgggtgc gtggcgcggg cggcagactg gcgagaaacg tcgcgccgta
    13381 aggacgcatc accagccgat tgtcacaaat taccaaaacc ccgcgatcgt cggcgtcgcg
    13441 aatcagtcgc ccgactccct gcttgagagt aatcaccgca tccggcagtt gtacttcatc
    13501 gaacgggtcg ccaccgcgca aacggcaatc ttccatgcgc gcttttaaca gtggatcatc
    13561 aggcgaggta aacggcaatt tgtcgataat caccaatgac aatgtatcgc cacgcacgtc
    13621 caccccctcc cagaaactac tggttgccac aagaagcgcg ttaccggcac tgacaaattg
    13681 ctgcaacagt tgccctttgc tggtttcccc ctgcaataat acgggaagcg tcatggtagc
    13741 gcggaactgc tcggccagat cgcgcatcat ggcgtgcgag gtacaaagca taaaacagcg
    13801 accgttgtta gcttcgatga tcgggcgcag catagccgcc agttgccgcg cggaacccgg
    13861 ttgattggtt tgcggcaggt tgcgcggcac acagagtaac gcctggcggc tgtaatcaaa
    13921 cgggcttggc aatagcaacg actccgcctg ttctatccca agccgcgagg tgaaatgatg
    13981 cagatcgtcg ttcaccgaca gcgttgctga ggtgaagatc cagctaccgg gcttttgcgc
    14041 cattaactct ttgaatttat ccgccacgct gagcggcgtg agagccagag taaaatggcg
    14101 cgaagtgcat tcgtaccagt agctgtagcc cggctgattg atctctttta gccgcttcag
    14161 ccgtgtgcga tacaacgtgg cgcgctcaaa tgccgcatcc agcaaggcgg aacgccccag
    14221 tgacagtttc gccacgtcat aacaaagttc cagggtgtca tcgagcagta aaaatgcccg
    14281 ctgaatttgc ggattagcta acagctcacg caggttaccg cgataacctg gttcaccgag
    14341 ttgcagacga aaatcctgcg cactctgggc aagacgatca gcgcactttt gtaactgctg
    14401 ggtgtctttt aattcggtgc ggtaagcgat ggtgatgtct tttgccaggt cgagcagttg
    14461 tcgactggag agtgactgac caaaatactg gctggcaatg tccggtagct ggtgggcttc
    14521 gtcgaagatc atgacgtccg cttccgggat caactcgcca aatccactct ctttaaccac
    14581 catatccgcc agaaagagat gatggtttac caccaccaca tcggcgtcca tcgctttttt
    14641 acgtgctttg accacaaagc aatctttata catcgggcag tcgctgccaa gacagttgtc
    14701 gttggtgctg gtaaccagcg gccacgcctg tgaatcttcc gccacgctga cgcaggtgct
    14761 gatatcacca tcgactgttt gattagacca ggagcgcagc aggatcacat cgcttaagat
    14821 ttgtaccggc agatcgcccc ccgccagcgc ctgctgttcg agacgttcga ggcagaggta
    14881 gtttgagcgc cctttcagca gcgccacgtt gcccgtatat ttcaatgcct ttgagactgt
    14941 tggcaaatcg cggctgtaga gctgatcctg caacgctttt gagccggtcg agataatgac
    15001 tttctttttc gcccgcagcg caggagccag gtaagcgtag gttttgcccg taccggttcc
    15061 tgcttccacc accagcggct ggcctttttc tatcgcctgg gtgacggcta ccgccatctg
    15121 tcgctgtggt tctcgcggct taaagcctgg tatcgctttc gccagctgac cgtctggtgc
    15181 aaaatcgtcc gtcacactac cccctgttga tttgaacagg gattatgtca ggatgagggc
    15241 gctttcgcca gttgaagtgg tgacggcgac ctcacattgt ggcagtcttt gcagcacgaa
    15301 aatggaagga taatgaggac aaaatgatga ctatcgttcg tatcgatgct gaagcccgct
    15361 ggtctgatgt ggtaatccac aacaacacgc tctactacac tggtgtaccg gaaaacctcg
    15421 acgccgatgc ctttgagcaa accgccaaca cgctggcgca gattgatgcc gtgctggaaa
    15481 aacagggcag caataaatcg agcattctgg atgccaccat tttcctggcc gataaaaacg
    15541 acttcgcggc gatgaataaa gcgtgggatg cctgggttgt cgcgggtcat gcgccggtgc
    15601 gctgcacggt acaagcgggt ttgatgaacc cgaagtataa agttgaaatt aagattgtgg
    15661 ctgcggtgta agctttatcg aagcaaaata agtcagacga taatttatcg ataatactgg
    15721 tcggttttac ataaatcgac cagagattaa gatattacc
//
LOCUS       DAZFRN010000114        15708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFRN010000114 DAZFRN010000000
VERSION     DAZFRN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.116"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            188..730
                     /gene="yfaZ"
                     /locus_tag="ACHKUB_003500"
     CDS             188..730
                     /gene="yfaZ"
                     /locus_tag="ACHKUB_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO6656470.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            830..2032
                     /gene="yfaY"
                     /locus_tag="ACHKUB_003501"
     CDS             830..2032
                     /gene="yfaY"
                     /locus_tag="ACHKUB_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO6656471.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2096..2557
                     /locus_tag="ACHKUB_003502"
                     /pseudo
     CDS             2096..2557
                     /locus_tag="ACHKUB_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2598..3500)
                     /gene="rpnE"
                     /locus_tag="ACHKUB_003503"
     CDS             complement(2598..3500)
                     /gene="rpnE"
                     /locus_tag="ACHKUB_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO6656472.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSPNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3694..4884)
                     /gene="glpC"
                     /locus_tag="ACHKUB_003504"
     CDS             complement(3694..4884)
                     /gene="glpC"
                     /locus_tag="ACHKUB_003504"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO6656473.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4881..6140)
                     /gene="glpB"
                     /locus_tag="ACHKUB_003505"
     CDS             complement(4881..6140)
                     /gene="glpB"
                     /locus_tag="ACHKUB_003505"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO6656474.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGKTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6130..7758)
                     /gene="glpA"
                     /locus_tag="ACHKUB_003506"
     CDS             complement(6130..7758)
                     /gene="glpA"
                     /locus_tag="ACHKUB_003506"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO6656475.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            8031..9389
                     /gene="glpT"
                     /locus_tag="ACHKUB_003507"
     CDS             8031..9389
                     /gene="glpT"
                     /locus_tag="ACHKUB_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO6656476.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9394..10470
                     /gene="glpQ"
                     /locus_tag="ACHKUB_003508"
     CDS             9394..10470
                     /gene="glpQ"
                     /locus_tag="ACHKUB_003508"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6656477.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10512..10751)
                     /locus_tag="ACHKUB_003509"
                     /pseudo
     CDS             complement(10512..10751)
                     /locus_tag="ACHKUB_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10749..10853
                     /locus_tag="ACHKUB_003510"
                     /pseudo
     CDS             10749..10853
                     /locus_tag="ACHKUB_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10933..11583
                     /gene="inaA"
                     /locus_tag="ACHKUB_003511"
     CDS             10933..11583
                     /gene="inaA"
                     /locus_tag="ACHKUB_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO6656478.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11637..11891)
                     /gene="yfaE"
                     /locus_tag="ACHKUB_003512"
     CDS             complement(11637..11891)
                     /gene="yfaE"
                     /locus_tag="ACHKUB_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO6656479.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(11891..13021)
                     /gene="nrdB"
                     /locus_tag="ACHKUB_003513"
     CDS             complement(11891..13021)
                     /gene="nrdB"
                     /locus_tag="ACHKUB_003513"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6656480.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13110..15395)
                     /gene="nrdA"
                     /locus_tag="ACHKUB_003514"
     CDS             complement(13110..15395)
                     /gene="nrdA"
                     /locus_tag="ACHKUB_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6656481.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            15523..15666
                     /gene="ypaB"
                     /locus_tag="ACHKUB_003515"
     CDS             15523..15666
                     /gene="ypaB"
                     /locus_tag="ACHKUB_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO6656482.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         3645 a         3881 c         4282 g         3900 t
ORIGIN      
        1 tgcacttatt attatttttg tgtgtatcgg taaagtgtgt ttattttttc ggcgtttaat
       61 tatttaagaa gtatttagtt acaataagtg actagatccc ttttttcgct cattgaatcc
      121 tctgtgccag gcagtacaat cccgcagctt tgggaatttt gattaattgc ttgtgggaaa
      181 ggggataatg aaaaaaattg cgctagcagg tctggccgga atgctgttgg tttctgcatc
      241 ggtcaatgca atgagcatca gcggccaggc gggtaaagaa tacaccaata ttggtgtcgg
      301 ttttgggact gaatcgacgg gcctggcttt aagcggtaac tggacacata acgacgacga
      361 cggtgacgtc gcgggcgtgg ggctggggtt gaatctgcct ctcgggccgt taatggcgac
      421 cgttggcgga aaaggcgttt acaccaaccc gaattacggc gatgaaggtt atgccgcagc
      481 ggtaggaggt ggtttgcagt ggaaaattgg caacagcttc cgtttgtttg gcgagtatta
      541 ctactctccg gattcgctct ccagcggtat tcaaagttat gaggaagcga atgctggcgc
      601 gcgttacacc attatgcgtc cagtcagtat tgaggcgggt tatcgctacc tgaatctgtc
      661 gggtaaagac ggtaaccgcg acaacgccgt ggctgacggc ccgtatgttg gggttaacgc
      721 cagtttctga tcccttccgg cgcggcattc tgtcgcgcca ttcagtttct tactacgcct
      781 tatctccctt tgcgttatag tgattcgata acaaaagcag gagataacca tgttaaaagt
      841 ggaaatgcta tccaccgggg atgaagtgtt acacgggcaa atcgttgaca ctaacgctgc
      901 ctggctggcc gattttttct ttcatcaggg gctgccatta tctcgccgca atacggtggg
      961 ggataacctt gatgacttag tcaccattct tcgcgaacgt agtcagcacg ccgatgtgct
     1021 gatcgttaac ggcgggctgg gaccgaccag cgatgattta agcgcactcg ccgctgcgac
     1081 agcaaaaggt gaaggcctgg tgctgcatga agcctggctc aaagagatgg aacgctattt
     1141 ccacgaacgt ggacgagtaa tggcaccgag caaccgtaaa caagcggagc tgcctgccag
     1201 tgctgaattt atcaataacc cggtaggcac cgcctgtggt tttgccgtgc agcttaatcg
     1261 ttgcctgatg ttctttactc ccggcgtacc gtcagaattt aaggtgatgg tcgagcacga
     1321 aatcctgccg cgtctgcgcg agcgtttttc tttaccacag ccgccggttt gtctgcgttt
     1381 aactaccttt ggtcgttcgg aaagcgatct ggcacaaagc ctggacactc tacaactgcc
     1441 gccgggcgta acaatgggct atcgctcttc aatgccgatt atcgaactga aactcaccgg
     1501 accggcaagc gagcaacagg cgatggaaaa actgtggctg gacgttaaac gtgttgccgg
     1561 acagagcgtg attttcgaag gcaccgaagg actgcccgcg cagatcagtc gcgaattgca
     1621 aaaccgccag ttcagcctga cgttgagcga gcaattcacc ggtggtttat tggctttgca
     1681 actttctcgc gcaggtgctc cattgctggc gtgtgaagtg gttccttcac aggaagaaac
     1741 cctggcgcaa actgcgcact ggattacaga acggcgggcc aaccattttg ccgggctggc
     1801 actggctgtt tcgggtttcg agaacgaaca tctcaacttt gcgctagcca caccagacgg
     1861 cactttcgct ctgcgtgtgc gtttcagcac tacgcgctac agcctggcta tccgtcagga
     1921 agtgtgcgca atgatggcac tgaatatgct gcgccgttgg ttaaacggcc aggatatcgc
     1981 cagtgagcat ggctggattg aggttattga gtccatgacc ttatctgtct gattccgcca
     2041 ctctgtgccg gtttactgca ccggcacatt ccccctccgg caaaacttgt ccaaccctcc
     2101 ctacggtgag cgtggtgttg gggccagtgt ggcacgggct gcgcgctggg gacgcattga
     2161 gaattacatg gcgcaagtca acgattcgct ttgtctgtta gtgcaggtgg aaagtaaaac
     2221 ggcactggat aacctggacg agatcctcga cgtcgaaggg attgatggcg tgtttattgg
     2281 accagcggat ctttctgcgt cgttgggcta cccggataac gccgggcacc cggaagtgca
     2341 gcgaattatt gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg ctggttttct
     2401 ggctgtggct cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact ttgtcgctgt
     2461 tggcgttgac acgatgctct acagcgatgc cctggatcaa cgactggcga tgtttaaatc
     2521 aggcaaaaat gggccacgca taaaaggtag ttattgatat caaaggccca tggggatcgg
     2581 ctgtgggcct gtgttaatta gtggttattc gctgccagat cggcttcgct tagctgggtg
     2641 gccgcgagca cctggtcacg atcaatgccc tgttccagca tgcgcaaagc aattttaatg
     2701 gcttgttcat gcataccttc ctgccagcca atttgatgcc cttcctgccg tagccgttca
     2761 gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt cggcaatctc ctcaataaaa
     2821 cgggtgaaac gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctgg
     2881 ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc tgtcgaccat gccgattaaa
     2941 tcgcggtcgc gaatatgctt ttgaatcagt tccagcagag ctatacgccg atgttgcatg
     3001 atctcatcgt caggtacgat ggtgatatcc accaacggaa aagcttcggt gtacaactgc
     3061 cgggcaagtt gcggatcgtc aaactcatcc agccagttag gtgagtacgg gtagggcgat
     3121 gtttcgccat gataaaacag caatggcacc accagcggaa ctctgtcata gcctttatcc
     3181 aggtgacgct gcatggcggc agtggcatag cgcattagcc gaaaagccat attcttttct
     3241 gcagagcttt gatgttcaat cacgcagtag atatagccgt caccgtcgct ggtttccacg
     3301 gaccacaaaa catccgagta gtaagcgcgt aaactttttt caataaaact agtgggttcc
     3361 aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt ctggtaaatg tatttcgaga
     3421 aaatcccgtg cggtttcggg tgtgaacata aaggttttaa acaccgcatc atgcggggag
     3481 gatgttgttg attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac
     3541 gtctatcccg ggcctttgac atctgtcagt ttcagccttt gcgagacatt ttcagaggaa
     3601 ttttcaaagc gttgcaacgc atacatggtg cgtggaatcg cgctcgcaaa aatgcccgat
     3661 aagcaatgtg cttaccgggc atcaaaaggt cggttaagcc agcgcctggg ccagtagcgt
     3721 aatcggatgt tcgcagcgaa ggctcgtgga catctcaatc tgccatttgc aggtttcgca
     3781 gtcggtgatg accagatccg cgccgctctc ttctatctgg cggaacagtg gcgcaccgat
     3841 ggcttgtgag gtggggtagt tctctttttt gaaaccgtaa gtacccgcaa taccgcagca
     3901 ctgggaatcc agcaccgtta actcaagccc cgggatttta cgcaacagct ccagggtgta
     3961 gagcgtccag cccatttttt ccatatggca cggagtgtga taaaccactt tcagcggcag
     4021 cggtttcagc ggtaacgttt tgccttcgtc cagcttgcgc cacagccagc gggttgccag
     4081 ttcgatatga tcgcgcaagc ctttgttgtc gacattcagc acttccgggt attcgtcgcg
     4141 cagggcaaat gtacaggttg aggaggtggc aatcaccgga atgcctttta ctcccacagc
     4201 ttcgcggatc gactctacat tcgtaattgc ctgtttgcgt gctttatcgg taaagccgtt
     4261 ggcgattagc ggtacgccgc agcatttttc tttgctgagc agttgtacac cggtacccat
     4321 tgcgttgagc actttaatta aatctttacc taactgcgga tggttgtagt taacgaagca
     4381 gccgtgaaag aaagcgacct ggtctttata ttgtgcttgc tgagccgcta tgctgcgata
     4441 ccagcgacgg aacgtgccga aggagtattt cggtagcgtg cggcgatgat cgatttttaa
     4501 cgccgcatca agcagctgcc gcaccggttt cagcgaggta gcggtgttga cgattggtgc
     4561 gaacggcgtg gaaacgctac ccatcaggtc ggtatgactc aacacaaaat tacgcagcga
     4621 cgggcgcgtg gtgtcatatt ttgcccgcgc gcgctggata atatcgccaa tcttcacatc
     4681 ggacgggcag gcgacttcac aacgtttgca gttgatgcaa tatttcagcg cctcgtcata
     4741 cagtgcgcca tctttcaaac gcagacgctc gccatccggc ccggcttgtt ttggccctgg
     4801 ataaccggga ttcacccggc tcaccgggca ggcggtggtg cagacggtgc acttaatgca
     4861 gttttcgaag ctggtgtcgt tcattgttgg cctcctgcgc gttgggcaat ctgttgagcg
     4921 gcatgtaaag cactgacggc acaaacaccg ccgccgcatc cctgggcgat gggatcaaat
     4981 ccgcccagca ccgaaccgat ggcaaacagg ttctcaatgg ttttccctgc ctgcgacggg
     5041 cgtagcgtct catcagtggt tacaccgaac tgctgccacg gttgcggcgc aaaaaaatcg
     5101 cccttatacc attcaccccg cgtggcggtt tgtagcacat caaggccgag aatcggctcg
     5161 cgaatgccgt tacgttccgc taccagtccg ccactaaaga aactgccgct ggcgagaacc
     5221 gcgaaacgtg gacgtagcgg aatatcggcg tgattgcggg tccagatttc attcactacg
     5281 ccatttttac aggtcacttt tttcacttca tcgcccggca tccacacgcc accctggcgc
     5341 acaaactggc gctgtaactg gttttgcaga cgaatgccca gcacggaagg cggcagcgtt
     5401 ggcaaaagca tcagtgaaca aggtagtttt tcattcaacc aacgccacag tttgtcatcg
     5461 gccaggccga agcaggcggg catcaggatc atttcgcagg tattggcgac aggaataagc
     5521 gcatcaagta acagcggcca gttttcttca ttatcaagga aacgggcgat attcacagcg
     5581 cgaaattcgg tggcgttatt gcgcaggaca tccagttccg gcagctctat ttctgcggtt
     5641 tcaaccgaaa gatccagttc gcgcaacgaa gccgccgcaa gatgcgcctg gaaatccatc
     5701 agcccgctta tccccactac acatatcttc ttcgcgggta gcggccagac gggaacttct
     5761 ggcgaactta accaggttgc acgcaaagtg ccgagcgggg taacgcgctg atgggccagt
     5821 tccacgctgc cctgcaattg cgcaccgctt tcagcgatca atgcctgcgc ctggcaagcg
     5881 agatcgagca cgcgttgtgg ttcgagaaga ctgtaaggat gggctggtgc ctgctgacgc
     5941 aaagattcca gtccactgtg aatgtctgtc accggttgac catcaggcag atggctcagc
     6001 aaatccagcg aaccggatga gaaatgcagc gcgctttgac cacgcgtgac aatggcacag
     6061 cgcaggccgt gtttttgcag ttgcaggcca cagagtaatc cggcgaggcc gccgcccata
     6121 atgacagtat caaagcgcat ctttctgctc cttctccaga ccacacagtc cctgataaac
     6181 ccagcgggta aattcgcttt cgcgcagtgc gtctccccag gcgatgggtt gcacgccttt
     6241 ccagcgttcg ttaaggaagg tggaaagttg ctcgatagat tgcgcggacg tcgtgacgtt
     6301 aaaacgttgc agcagtccgg cagcgcggca ggcgcagagt tcgccctggc aggtacccat
     6361 ccccacacgg gtacgacggc gtaaatccag caggctatta acgtttaaat tttctaccgc
     6421 gtactgaact tcacccgcag ttaccgcttc gcactcacat accaggctac gatgcagacg
     6481 gccttcgctc agccaggcag gcgtgcgatc gccatgacga taaaccgcag aaccgcgcag
     6541 cggggcaggc agggagatga ctttacgcaa ggtaacttca gcgggttctt gtgaaccagg
     6601 cagtgccaga tcggcagtcg tacaggggcg cgtgttgccc agtttgcggc ataccgcgtc
     6661 ggtagcccat tcagccatca gccgataggt catcagtttg ccaccggtga tggtgataaa
     6721 tccgtccaga ccatcgcgtt cagcatggtc gagcagcacg atgccacggc tgacgttacg
     6781 tccgctcggg tcgtcatcgc tggcaaccag cgggcgcacg ccagaatagg cccgcaaaat
     6841 gcgcgttttc gccatcacgg gggccagttt ttccccttca cgcagcagaa tatcaacctc
     6901 ttctgccgtc actcgattat cgtcaatctc gttgtaatca atacgtaaag aggtggtgcc
     6961 aatcagcgaa atggtatcgc caggcaccag aatatcggcg tcggaaggtt tacggcagcg
     7021 gttgatcaca tgctggttaa tgcggtgatc catgatcagc agcgatcctt tcgccgggaa
     7081 catgcgaatg cgcagatcgg catattcggc aatgtgttgc ccccagatcc cagcggcatt
     7141 aaccacgaca ggtgcatgaa gggcctgagt ttcgccggtg agatggttac gtacacgaac
     7201 accgcacacc gtcgcgcctt cacgaatcag ccccgtgact tcatgagcgg taaggataac
     7261 ggcaccgtgt tctttggcat ccagcatgtt tgctgcggtc agacgaaatg gatcaacggt
     7321 gccatccgga actttcaccg cgccaatcag tgccgggtta acggcaggtt cgataatgcg
     7381 cgcttgctgc gggtctatag cttctgcgct gatccctgct tcttcgcagg cgcgaataaa
     7441 agtggcctgg aatgagaggt catcttccgg cagggtgata aacaggccgt tggttggttc
     7501 aacgcagtga cgtgcaatgc gtttcaggat ctggttttca ctaatgcatt cgcgggccga
     7561 ttccgcatcg gttaccgcat agcgcgcacc gctgtgcagc aggccgtggt tacgcccggt
     7621 ggcaccggtt gcgatgtcgt ggcgctcaac caaaatcacg cgcagcccgc gcagggcaca
     7681 gtcgcgggca atcccggctc ccgttgcgcc gccaccaatg ataatcacgt cacttgattg
     7741 cgagtcgcga gttttcattg ttatccctct gaagttcgtt ttttaccatt tagccatagt
     7801 aaaaacatga attgtttgat ttcgcgcata ttcgctcata attcgaaagt gaaacgtgat
     7861 ttcatgcgtc attctgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca
     7921 tttaatttct gaatgttttc ttaacatcgc ggcaactaaa gaaacggcag gttctcccac
     7981 tgaatcagtc tgttaatcat aaataagacc acgggccacg gaggctttca atgttgagta
     8041 tttttaaacc agcgccacac aaagcgcgct tacctgctgc ggagatcgat ccgacttatc
     8101 gtcgattgcg ctggcaaatt ttcctgggga tattctttgg ctatgcggct tactatttgg
     8161 ttcgtaagaa ctttgcgctt gctatgcctt atctggtcga gcagggattc tctcgcggtg
     8221 atttaggttt cgcgctttcg gggatctcga tcgcttatgg attttcgaaa ttcataatgg
     8281 gttcggtatc agatcgctcg aatccgcgcg ttttcctccc cgcaggtttg attctggcgg
     8341 cggcagtgat gttgtttatg ggctttgtgc catgggcgac gtcgagcatt gcggtgatgt
     8401 ttgtactttt gttcctctgc ggttggttcc aggggatggg gtggccgccg tgtggtcgta
     8461 ctatggtgca ctggtggtcg cagaaagaac gtggcggcat tgtgtcagtg tggaactgtg
     8521 cgcacaacgt tggtggtggt attccgccgc tgctgttcct gctggggatg gcctggttca
     8581 atgactggca cgcggcgctc tatatgcccg ctttctgcgc cattctggtg gcattattcg
     8641 cctttgcgat gatgcgcgat accccgcaat cctgtggctt gccgccgatc gaagagtaca
     8701 aaaatgatta tccggacgac tataacgaaa aagcggaaca ggagctgacg gcgaagcaaa
     8761 tcttcatgca gtacgtactg ccgaacaaac tgctgtggta tatcgccatc gccaacgtgt
     8821 tcgtttatct gctgcgttac ggcatcctcg actggtcacc gacttatctg aaagaggtta
     8881 agcatttcgc gctggataaa tcctcctggg cctacttcct ttatgaatat gcaggtattc
     8941 cgggcactct gctgtgcggc tggatgtcgg ataaagtctt ccgtggcaac cgtggggcaa
     9001 ccggcgtttt ctttatgaca ctggtgacca tcgcgactat cgtttactgg atgaacccgg
     9061 caggtaaccc aaccgtcgat atgatttgta tgattgttat cggcttcctg atctacggtc
     9121 ctgtgatgct gatcggtctg catgcgctgg aactggcacc gaaaaaagcg gcaggtacgg
     9181 cagcgggctt taccgggctg tttggttacc tgggcggttc ggtggcggcg agcgcgattg
     9241 ttggctacac cgtggacttc ttcggctggg atggcggctt tatggtaatg attggcggca
     9301 gcattctggc ggttatcttg ttgattgttg tgatgattgg cgaaaaacgt cgccatgaac
     9361 aattactgca aaaacgcaac ggaggctaat ggcatgaaat tgacgctgaa aaaccttagc
     9421 atggcgatca tgatgagcac tatagtcatg ggaagcagtg caatggcggc ggacagcaac
     9481 gaaaaaatag tcatcgccca tcgcggtgcc agtggatatt tgccggagca tacgctgcca
     9541 gcaaaagcga tggcgtatgc gcagggagcg gattatctgg aacaggattt ggtgatgacc
     9601 aaagacgacc atctggttgt tctgcatgac cattatctcg atcgtgttac tgatgttgcc
     9661 gatcgtttcc cggatcgggc gcgcaaagac ggtcgttact acgcgataga tttcacgctg
     9721 gatgaaatta agtcgctgaa atttaccgaa ggtttcgata ttgaaaacgg taaaaaagtg
     9781 cagacttatc cggggcgttt cccaatgggt aagtccgact tccgggtgca cacctttgaa
     9841 gaagagattg aatttgttca ggggttaaat cactctaccg ggaaaaatat cggtatttat
     9901 cctgaaatca aagcgccgtg gttccatcat caggaaggga aggatattgc ggcaaaaacg
     9961 ctggaagtgc tgaagaaata tggttacacc ggtaaggacg acaaagttta tttgcaatgt
    10021 tttgatgctg atgagctgaa gcgtattaag aatgagctgg aacccaaaat gggcatggat
    10081 ctcaatctgg tacagctgat tgcctatacc gactggaatg aaacgcagca gaaacagccg
    10141 gatggaagct gggttaatta caactacgac tggatgttta agccgggtgc tatgaaacag
    10201 gtggcggaat atgccgacgg tatcgggccg gattaccata tgttgattga ggaaacatcg
    10261 cagccgggta atatcaaact cactggcatg gtgcaagatg ctcagcagaa taaactggta
    10321 gtgcatcctt ataccgtgcg gtcagataaa ctgcctgaat acactactga tgtgaatcag
    10381 ttatatgatg ctctgtataa caaagcgggt gtaaatgggc tgtttactga tttccctgat
    10441 aaggcagtaa aattccttaa taaagagtaa tgctactgca ggatggcgtt catgcgccat
    10501 cctgtctcac atcaaatttt tccattcacc acgagttgtt ccgtacacgt ttgtagtaaa
    10561 aacagtggac tgccttctac cggcttttct ttagccaaca acgaaatctg tcgatagaag
    10621 gttggttcgt gaggaacgga acacaattca cctgcaatgc ttttcagcgt taactctggt
    10681 tgtaaggcga tgcctaatcc ctgtcgaata aacttcatcg ctgtatccta ctgattaaat
    10741 tcacatttta tctactgggc gcactattag tggtagtggc tggtggaatc tatgcgctgc
    10801 aaatgaaggg taaaagcggt gtcacgctag tagtggcaaa agaaattcat taatacgaca
    10861 cgtttcatta agattttcct caggtaacca cctatagtca ttgtcgttaa taaaaatgac
    10921 aggcgatagg gtatggcagt ttcagcaaag tacgacgaat ttaatcactg gtgggcaaca
    10981 gagggcgact gggttgaaga gcctaactat cgccgtaatg gtatgagcgg ggtgcaatgc
    11041 gtcgagcgca acggcaaaaa gctgtatgta aagcgcatga cgcatcatct gtttcattct
    11101 gtacgttatc cgttcggccg accaacgatt gtccgtgagg ttgcggtaat taaagaactg
    11161 gaacgggccg gtgtcatcgt gccgaagatc gtttttggtg aagcggtgaa aattgagggt
    11221 gaatggcgag cgttgctggt gactgaagat atggcggggt tcatcagcat tgctgactgg
    11281 tatgcccagc atgcagtatc gccttattct gacgaagtac ggcaagccat gttgaaagca
    11341 gtggcgctgg cgtttaagaa aatgcatagc attaatcgtc agcatggctg ttgttatgtt
    11401 cgccatatct atgtgaaaac agaaggtaag gcagaagctg gttttcttga cctggagaaa
    11461 agtcgtcgcc gcttgcgtcg cgataaagcg attaatcatg acttccgcca gctggaaaaa
    11521 tatctggagc caatccccaa agcggactgg gagcaggtga aagcgtatta ctacgccatg
    11581 taatcgttgt aggccagata agacgcataa gcgttgtatc tggcactaca accaattcac
    11641 atctcgattt caatatcgcc ttttgcccgg caacaacagg gcaaaatttc ccccggctga
    11701 ataaaggcta acggttcggc aatccagtca acttgacctg caaccaggcg tgtgcgacag
    11761 gagccgcagt aaccttcgcg acactgatac tcaaccgcca cattgtggga ttccagcgcc
    11821 gccagaaggg aagggtgttc atcctggcac agcagttgtg tgccagtgat gcgcagggta
    11881 acgcgggcca tcagagttgg aagttactca aatcgtcggt gtccacttcc gcgtcaatct
    11941 gcccgaccag ataagaactg acttccactt cctgcggagc aacctgcacg ttatcagaca
    12001 ccagccaggt gttgatccat gggatcgggt tggagcgcgt ctggaacggc agatccaaac
    12061 cgactgcctg catacggata ttggtgatgt attcaacgta ctggcagaga atatctttat
    12121 tcagaccaat catcgaaccg tcacggaaca gataatccgc ccagtctttc tcctgttgag
    12181 ctgcctgaac aaacaggtca tagcactcct gcttacactc ttcggcaatt tccgccatct
    12241 caggatcgtc cgcgccgctg cgcagcagat tcagcatatg ctgggtgccg gtcaggtgca
    12301 gggcttcgtc gcgggcaatc aggcgaataa ttttggcgtt gccttccatc aattcgcgtt
    12361 ctgcaaaggc aaaggaacaa gcaaagctga cgtagaagcg gatcgcttcc agcgcgttaa
    12421 cgctcatcag gcaaagatac agttttttct tcagctcgcg caggctaacg gttacggttt
    12481 taccgttaac ggtgtgggtg ccttcgccca gaaggtgcca gtagctggtc atttcgatca
    12541 gttcatcgta atagctggag atcccttccg cacgtttctg gatctgctcg ttggtgacga
    12601 tatcgtcaaa cacaacagac ggatcgttaa cgatattacg aatgatatga gtataggaac
    12661 gggaatgaat cgtttctgag aacgcccagg tttcgaccca ggtttccagt tccggaatag
    12721 aaataagcgg caatagcgcc acgttcggac tacgaccctg aatggagtcc agcagcgtct
    12781 gatatttcag gttgctgata aagatgtgtt tttcgtgctc cggcagcgcc tggtaatcga
    12841 tacggtcgcg ggagacgtca acttcttccg gacgccagaa gaaagagagc tgcttttcga
    12901 tcagcttttc gaagatgtca tatttttgct gatcgtagcg agccacgttg accggctgac
    12961 caaaaaacat cggttcttta agctgatcat ttttcgtctg tgaaaaggtg gtatatgcca
    13021 tgagtgtgtc ctgtggtttt attacccctc accctaaccc tctcccgcaa ggggagaggg
    13081 gacggtttgg tgcaaaggct gggggattat cagatcttac atgcgccgct ttcgcagcca
    13141 tcgtcctgga ttgacggcac cagatcgtcc tgcgcgtctt cagcgccgtc acgggtgttc
    13201 tgataataca gcgttttgac cccgaatttg taggcggtga gcaggtcttt cagcaactgc
    13261 tgcatcggca cttttcctga cgggaaacgt gacggatcgt agttggtgtt agcagagatc
    13321 gactgatcga taaatttctg catgatacct accagttgca ggtagccatc gttacccggc
    13381 atttcccaca gcagctcata ggcgtcgtgc aggtgctcgt agtccggcac cacctggcgc
    13441 aaaataccgt ctttcgacgc tttgatgctg acgtaaccgc gcggcggttc aataccgtta
    13501 gtggcgttag agatctgcga agaggtctcg gacggcatca gggcagaaag cgtggagtta
    13561 cgcaggccgt gcgttttgat tgattcacgc aatgcttccc agtcgtaatg cagcggctca
    13621 ttagcgatgg catccagatc tttcttatag gtatcgatcg gcaggatccc tttcgcgtaa
    13681 gtggtttcgt taaaccacgg gcacgcgcct tgctctttag ccagctcatt agacgctttc
    13741 agcaggtaat actgaatcgc ttcgaaggtt ttatgcgtca ggttgttggc gctgccgtcg
    13801 gagtagcgtt taccgtgctt cgccagatag taagcgaagt taatcacacc aatacccagc
    13861 gtacgacgcc ccatcgctcc acgtttggcg gccgggatcg ggtaatcctg ataatccagc
    13921 agcgcgtcaa gtgcacgaac cgccagaatt gccagctctt ccagttcatc caggctatta
    13981 attgcgccca ggttgaaagc agacagcgta cacagcgcga tttcaccgtt ctcgtcgttg
    14041 acgtcgttca gcggtttggt cggcagggct atctccaggc acaggttaga ctgacgcact
    14101 ggcgcgatgg ccggatcaaa cgggctatgg gtattgcagt ggtcaacgtt ctgaatatag
    14161 atacgaccgg tagacgcacg ttcctgcatc atcagcgaga acagctcaac ggctttcaca
    14221 cgctgcttgc ggatgctgtc gtctttctca tatttggtat acaggcgttc aaactcttcc
    14281 tgatcggcga agaatgcgtc gtacagcccc ggtacgtcgg acgggctgaa cagggtgata
    14341 tcttcacctt tcagcaggcg ggtatacatc agtttgttga tctgtacccc gtagtccata
    14401 tggcgcacgc ggttgccttc cacaccacgg ttgtttttca acaccagcag gctttccact
    14461 tccagatgcc acatcgggta gaacagcgtt gccgcaccgc cgcgcacgcc gccctgagag
    14521 caggatttca ccgctgtctg gaaatgtttg tagaacggaa tacagccggt atggaacgct
    14581 tcaccaccgc gaatcgggct acccagcgca cgaatacgcc cggcgttgat gccgatcccg
    14641 gcacgctggg aaacgtattt cacaatcgcg ctggaggtag cgttgatgga atccagactg
    14701 tcaccgcact cgatcagtac gcaggagctg aactgacgag tcggggtacg cacgccggac
    14761 atgattggcg tcggcagcga aattttaaat gtggaaaccg cgtcgtaaaa acgcttcaca
    14821 tattgcaggc gcgtttcacg cgggtagttc gagaacaggc acgcggcaac cagaatataa
    14881 aggaactggg cgctttcgta gatttcgccg gtcacgcggt tctgtaccag atatttgcct
    14941 tccagctgct taacggcagc ataagagaag gtcatatcac ggtcgtgatc gataaacgtg
    15001 tccatctgct tgaactcttc ttccgtgtag tcttccagca gatgattatc gtatttgccc
    15061 atctcgacca ttttcaccac gtggtcgtac agcgcaggcg gctcaaactg gccgtaggct
    15121 tttttacgca ggtggaagat cgccaggcgc gcggcgagat actgataatc cggcgcatca
    15181 cgggagatca ggtctgcggc cgccttgata atggtttcgt ggatgtcaga ggtcttgata
    15241 ccgtcataaa actgaatgtg ggagcgcagc tcgacctggg aaatcgaaac gttatgcagt
    15301 ccttctgccg cccaatccag aacgcgatgg attttgtcga gattgatgcg ctctgtgcta
    15361 ccgtcgcgct ttgtcaccag cagattctga ttcatgtatg tcgtacctgt ttttggaatt
    15421 ctttccgcaa tagtttcatg cagactacct gtagtaaggg aagctgctta gaacactata
    15481 tatagggggt atgtttggga ttcactgcaa gatagtgtga aaatggccct cttgcaagtg
    15541 cataactttg tggataactc aggaaggaaa aagtggcttt cgcgcacctt aggtcagaca
    15601 aggtgtccgg gaaagtcaat gggaagagaa aaatttgtta aaaataactg ttcggcgatt
    15661 tcttgaacgg cagaataggt tggataaatt gatatagatg gtttatta
//
LOCUS       DAZFRN010000115        15691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFRN010000115 DAZFRN010000000
VERSION     DAZFRN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.117"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            68..652
                     /locus_tag="ACHKUB_003516"
     CDS             68..652
                     /locus_tag="ACHKUB_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656483.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            757..1497
                     /locus_tag="ACHKUB_003517"
     CDS             757..1497
                     /locus_tag="ACHKUB_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO6656484.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1532..4132
                     /gene="htrE"
                     /locus_tag="ACHKUB_003518"
     CDS             1532..4132
                     /gene="htrE"
                     /locus_tag="ACHKUB_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO6656485.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4149..4718
                     /locus_tag="ACHKUB_003519"
     CDS             4149..4718
                     /locus_tag="ACHKUB_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656486.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4733..5335
                     /gene="yadL"
                     /locus_tag="ACHKUB_003520"
     CDS             4733..5335
                     /gene="yadL"
                     /locus_tag="ACHKUB_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO6656487.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5362..5958
                     /locus_tag="ACHKUB_003521"
     CDS             5362..5958
                     /locus_tag="ACHKUB_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO6656488.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     TGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNFGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5942..7285
                     /locus_tag="ACHKUB_003522"
     CDS             5942..7285
                     /locus_tag="ACHKUB_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO6656489.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            7398..8192
                     /gene="panB"
                     /locus_tag="ACHKUB_003523"
     CDS             7398..8192
                     /gene="panB"
                     /locus_tag="ACHKUB_003523"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO6656490.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8204..9055
                     /gene="panC"
                     /locus_tag="ACHKUB_003524"
     CDS             8204..9055
                     /gene="panC"
                     /locus_tag="ACHKUB_003524"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO6656491.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9129..10031)
                     /gene="rpnC"
                     /locus_tag="ACHKUB_003525"
     CDS             complement(9129..10031)
                     /gene="rpnC"
                     /locus_tag="ACHKUB_003525"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO6656492.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSRKDVAEMTNLSLAEIDKVINLI"
     gene            10305..10685
                     /gene="panD"
                     /locus_tag="ACHKUB_003526"
     CDS             10305..10685
                     /gene="panD"
                     /locus_tag="ACHKUB_003526"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO6656493.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10689..11918)
                     /locus_tag="ACHKUB_003527"
     CDS             complement(10689..11918)
                     /locus_tag="ACHKUB_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO6656494.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11982..12422)
                     /locus_tag="ACHKUB_003528"
     CDS             complement(11982..12422)
                     /locus_tag="ACHKUB_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6656495.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12527..13297)
                     /locus_tag="ACHKUB_003529"
     CDS             complement(12527..13297)
                     /locus_tag="ACHKUB_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6656496.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13294..14220)
                     /locus_tag="ACHKUB_003530"
     CDS             complement(13294..14220)
                     /locus_tag="ACHKUB_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6656497.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14329..14991
                     /gene="can"
                     /locus_tag="ACHKUB_003531"
     CDS             14329..14991
                     /gene="can"
                     /locus_tag="ACHKUB_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO6656498.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15032..15568)
                     /gene="hpt"
                     /locus_tag="ACHKUB_003532"
     CDS             complement(15032..15568)
                     /gene="hpt"
                     /locus_tag="ACHKUB_003532"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO6656499.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
BASE COUNT         4336 a         3717 c         3581 g         4057 t
ORIGIN      
        1 tttgtgtcat gaatagtcat tcttcaaacc agtagtctga ctaatggaat tttattcaag
       61 gatttttatg tctaaaaaat taggttttgc cctgtctggt cttatgctgg caatggttgc
      121 aggtactgct tctgctgata tggatggtgg tcagttaaat atcagtggtc tggttgttga
      181 taacacctgt gaaacccgcg ttgacggcgg taataaagat ggtctgatcc tgctgcaaac
      241 tgcaactgtt ggtgaaatcg atgctggcgt gctgaatgac accgttggcg ctaaagccaa
      301 acctttcagc atcaccgttg actgcagcaa agcaaatccg aatccaggta gtacagctaa
      361 aatgaccttc ggttctgttt tctttggtaa tagcaaagga acgctgaaca acgacatgtc
      421 tattaacaac ccgtctgacg gcgtgaatat cgctctgcac aatattgatg gttccactat
      481 caaacaggtt caaattaaca accctggcga tgtgtatact aaagccctgg atgcaacgac
      541 aaaatctgct gtttatgatt ttaaagcgtc ttacgttcgt gccgtcgcag accaaacagc
      601 aactgctggt tatgtaaaaa ctaacactgc atacaccatt acttatcagt aagtattcac
      661 caattaaatt gattgaatga atatacaggg aatagtgtta ataatttcta tttttatatt
      721 attccctgtt ttaattaact ctgtcaggga tggtttatgt tttttaacac caaacataca
      781 acagctttat gcttcgtaac ctgtatggct tttagttcat cgtctattgc ggacattgtc
      841 atttcgggta ctcgcgtaat atataaaagc gatcaaaaaa gtgtcaacgt acgtctggaa
      901 aataaaggga ataacccgtt gcttgtccag agttggttag atactggcga tgacaacgct
      961 gagcctggca gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc
     1021 aaacgtgggc aaacaatcaa attaatgtac acagccagca cctcactgcc taaagacaga
     1081 gagagcgtgt tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc
     1141 gcgaatcaaa gtctgctgca actggcattt cgcacacgta taaaactttt ctatcgcccg
     1201 gatggattga agggaaatcc ctctgaagcc ccgttagccc tgaagtggtt ctggtcaggt
     1261 tcagaaggta aggcgtcatt gcgagtgacc aatccaaccc cttactacgt ctcttttagc
     1321 agtggtgatt tagaagctag cggtaaacgc tatccgattg atgtgaaaat gattgcacca
     1381 tttagtgatg aggtcatgaa agtcactggc cttaatggca aagcaagttc tgcaaaagta
     1441 catttttacg ccattaatga ttttggtggc gccattgaag gtaatgccag tctgtaatca
     1501 ggcaaggata taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca
     1561 taatcatctg acccgtatcg ctacgttttg cgcgctgctg tattgcaata gtgctttctg
     1621 tgctgaactt gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga
     1681 tctcagccgg tatagtgaag gtaaccctgc tataccgggt atgtacgacg tcagtgttta
     1741 tgtcaacgat caacctatta ttaaccaaag cattacattt attgaaattg aaggaaaaaa
     1801 aaacgcacaa gcctgtatca cacttaagaa tttattgcag tttcatataa attctcccga
     1861 tataaataac gaaaaagccg ttctgcttgc cagggatgaa acactcggca attgccttaa
     1921 tttgacggaa attatccctc aggcttctgt tcgttatgac gttaacgagc aacgtctgga
     1981 tatagacgtt cctcaggcat gggtaatgaa aaattaccaa aactatgttg atccatcgtt
     2041 atgggaaaat ggcattaatg cggccatgtt gtcatacaac ctcaacggat atcatagtga
     2101 aactcctggt cgaagaaatg acagcattta tgctgctttt aacggtggga tgaatttagg
     2161 agcatggcga ctgcgtgcct caggcaacta caactggatg accgattctg gcagtaatta
     2221 tgattttaag aatcggtata tacagcgtga tatcgcctcg ctgcgttctc aactcattct
     2281 tggtgagtct tatacaacgg gcgaaacctt tgattctgtc agtatccgcg gcattcgttt
     2341 atacagcgac agccgcatgt tgcctccgac attagcgagt tttgcgccta tcattcatgg
     2401 cgttgctaat accaacgcca aagtcactat tacgcaaggt ggctataaga tttatgaaac
     2461 gactgtgccg ccaggcgctt tcgtcattga tgatctgagt ccgtcaggtt acggcagcga
     2521 tcttattgtt actgtcgaag aatccgatgg ctcaaagcgg acattctcgc aacctttttc
     2581 atcggtcgtt caaatgttac gccctggcgt tggacgttgg gatattagcg gcggccaggt
     2641 cttaaaagat gatattcagg atgagcctaa tttatttcag gccagctact actacggtct
     2701 gaataactat ctaacgggtt atactggtat tcagattacc gataataact ataccgctgg
     2761 tttgttaggt cttggtctga atacttcagt cggtgcattt tctttcgatg tgactcactc
     2821 caatgttcgt ataccggatg ataaaacata tcagggacaa agttatcgtg tttcctggaa
     2881 caagttattc gaagaaacaa gtacttcact gaatatcgcg gcctatcgct attcgacaca
     2941 gaattacctt ggtcttaatg atgcactaac tctaattgat gaagtgaaac atcccgaaca
     3001 agatcttgaa ccgaaatcca tgcgtaatta ctcgcgcatg aaaaatcagg ttacggtcag
     3061 tattaaccaa ccgctaaaat ttgagaaaaa agattacggt tcattttatc tttccggaag
     3121 ttggtccgat tactgggctt ccggacaaaa tcgtagcaat tactctatcg gctacagtaa
     3181 cagtgcatcc tggggcagct acagtgtcag tgcccagcgt tcatggaatg aagacggcga
     3241 cactgacgat agcgtttatc tcagtttcac cattcctatt gaaaaattac ttggcactga
     3301 acaacggact tcaggtttcc agagtattga tactcaaatg agcagtgact ttaagggtaa
     3361 taaccaactc aacgtcagta gcagtggcta tagtgataac gctcgcgtca gttatagcgt
     3421 aaatactggc tatacgatga ataaagccag caaagatttg agttatgttg ggggttatgc
     3481 cagctatgaa tcaccgtggg gaaccctggc aggttcagtt tctgcaaata gcgataacag
     3541 tcgtcaagtg tctctcagca ccgacggtgg ttttgtattg catagcggcg gcctgacctt
     3601 cagtaatgac agttttagcg actccgatac actggcggta gttcaggctc caggtgctca
     3661 aggagcgcga ataaattatg gcaacagtac tatcgatcga tggggttatg gtgtcaccag
     3721 cgctctttct ccttatcatg aaaaccgtat cgcgctggat atcaacgatc ttgagaacga
     3781 tgttgaatta aaaagtacca gtgcagtagc tgtaccgcgt cagggttcag tcgtctttgc
     3841 tgattttgaa accgtgcaag ggcaatcagc cattatgaac atcacacgaa gtgatggtaa
     3901 aaatattcca tttgctgcag atatttatga tgagcaaggc aatgtcattg gtaatgttgg
     3961 acagggtgga caagcatttg ttcgtggtat tgagcagcag ggaaatatca gcattaaatg
     4021 gctcgaagaa agtaaacccg taagttgtct tgcgcattat caacaaagtt cagaagcaga
     4081 aaaaatagcg caatctatta ttctgaatgg aattcggtgt cagattcagt aactacaagg
     4141 gacgtcaaat gataaaaata acgccacata aaatagcgat actgatggga atattattat
     4201 caccatcagt atttgcaacg gatattaatg tagagtttac agccactgtc aaagcgacaa
     4261 cctgtaacat cacacttact ggtaataacg tcacgaatga tggcaataat aactacacat
     4321 tgagaatccc taagatgggt ctggataaga tcgcgaataa aacgacagaa tctcaggctg
     4381 attttaaact ggttgctagt gggtgcagca gtggcatcag ttggattgat accactctga
     4441 ccggaaatgc atcatcaagc tcacctaagc ttattatacc gcagtctggt gattcatctt
     4501 cgacgacaag taatatcggt atgggtttca aaaaacggac tactgatgat gccactttcc
     4561 ttaaacctaa cagtgcggaa aagatacgct ggagcacaga cgagatgcag cccgataagg
     4621 gtcttgaaat gaccgttgcg ctacgtgaaa cagatgcagg gcaaggcgta ccggggaatt
     4681 ttcgtgcact ggcaacgttt aatttcatct atcaataaga aggataatta agatgacatt
     4741 taaaaattta cgttatggat tatccagcag tgtcgttttg gctgcctcat tgttcagcgt
     4801 accctcttat gcggcaacag acagcattgg gctgaccgtg attactaccg tagaaatagg
     4861 tacttgtact gctacattag taaatgactc tgatcaggac atttctgttg ttgactttgg
     4921 cgatgtatat atttctgaaa tcaatgccaa gaccaaagtt aaaacattca aactcaaatt
     4981 caaagactgt gcgggtatcc ccaataaaaa agcacaaata aaattaacca agcgagccac
     5041 atgcgaaggt actgctaatg acggtgcggg gttcgcaaat ggatccacag ccgcagataa
     5101 agcaagtgcc gtcgccgttg aagtctggag caccgaaact ccggcaacag ggagcgcaac
     5161 acaatttagt tgtgtgacac cagcatctca agaggtcact atctccaatg cagccaacgc
     5221 ggttgtttat tatccgatga gtgctcgact ggtcgtcgaa aaaaataaaa ccgtaagcaa
     5281 tgtcactgcg ggtaaatttt ctgcacctgc cacatttaca gtaacctata actaacagac
     5341 tgacagatcc aggaactata tatgcatccc actcaacgta agctgatgaa gagaataatt
     5401 ctgtttctgt cactacagtt tcccattacc tatccagcca tcgctgggca ggatattgac
     5461 cttgttgcca atgtaaaaaa cagcacttgc aaaaccggaa tcagtaacca gggtaatatt
     5521 gatcttggcg tcgttggggt gggatatttt tcagataatg ttactcctga aagctatcaa
     5581 ccaggtggaa aagagttcac tgtcacagta tcagactgtg cattacaggg aactggcgat
     5641 gtgcttaatc agttacatat tgattttaga gcccttagcg gtgtcatggc tgcaggttca
     5701 aggcaaatat ttgccaatga agtcgcaacc ggggctaaaa atttcggtgt cgttctcttt
     5761 tccattcagg acccgaccaa tatattcaat gttattagtt cagcaggaaa ttctcgttcc
     5821 gtgtatccag taatgacttc tgccctgcat aattcttcat ggaaatttta tgccagaatg
     5881 caaaaaatcg atcccgcgct ggatgtcata tctggccagg taatgagtca tatattagtt
     5941 gatgtgtact acgaataata gatcaaaaca aataactgcg actgttacag cagtagatgg
     6001 tggagtgata atgaagaaat tcttcagaca ttttcttttt ttaattctgt gcttgagttg
     6061 ttataccgcg agtgctggca ctgatgataa cgtaggatat attgttggga atagttacgg
     6121 cgttggtccg tcagatcaaa aatggagaga aacggggccg aatggcgatg ctactgtcat
     6181 attcagatat gcaacaagta caaataattt agttttctac aaaccgacgc agcttggacc
     6241 tacaggtgta aaattacagt ggagtcagtt agataccgct tctggtggtg gttttcttta
     6301 ttgcaacaga agtgatagca caagtggtag cgcaatgcgt attgaaaatg caatggttga
     6361 ctcaggtaaa atgtatggct cccataaatt atttaataca tcagttcctg gtttgtatta
     6421 cacattatta atttcaaaca tgtggtcagc ttacggtacc gtaactaacg ttagttcacc
     6481 tgggatatac attggtgact ctgcagaaca atatttttcg tggtataatc caagcgaaga
     6541 cgtgttatat tggagttgca ataatgcgaa tagcacccgt aaatactggg ctgtaggtgg
     6601 tatttatcag acccttacaa ttgaattcta tacagataca aactttgatc caacagtaac
     6661 tcaacagatc aaattatcca gctcatcaaa ttatctgtat tcatttaaag cctatggcgc
     6721 aggccaaggt ataaatgagc atagttattt tatcaaaatc gatttcgatc tgctcaatgt
     6781 caagttgact aatcccactt gttttaccgc tatgcttagt ggaacttctg tgactggttc
     6841 tacggtaaaa atgggtgaat atagtgcaga acaaatcaga aacggtgcca caccggttcc
     6901 ttttgatatt tcacttcaaa actgcgttcg tgtgactaat attgagacaa aattagtttc
     6961 aacaaaggtt ggtactgaaa acgggcaact ccttggtaat actctcacag gtaatgacgc
     7021 agcaaaagga gtcggtgtac tcatagaagg tttagcaact agtaaaaatc ctctaatgac
     7081 attgaaacct aatgattcaa attctgttta taaagattac gacccgagag gcaaagacga
     7141 tacaacagga ggagtttacc cggatcaaga tacaggtata acataccctc tccatttcca
     7201 ggccacgcta caacaggatg gaactatacc aatagaagct ggtgagttta aagccaccag
     7261 tactttccag gtaacctacc cttaataagt ccaccgcacc gccatcatct ggcggtgcgg
     7321 taattgataa atcattgccc ccaccaatgt gactgataaa atgccaggca acacgacatc
     7381 atttatcagg acacgttatg aaaccgacca ccattgcttc acttcagaaa tgtaagcagg
     7441 ataaaaagcg tttcgcgacc atcaccgctt acgactacag cttcgcgaaa ctatttgctg
     7501 atgaaggact taacgtcatg ctggtaggcg attcgctggg catgacggtt cagggccacg
     7561 actccaccct tcccgttacc gtcgaggata tcgcctatca taccgccgcc gtacgtcgcg
     7621 gcgcgccaaa ctgcctgctg ttggctgacc tgccgtttat ggcgtatgcc acgccggaac
     7681 aagcctttga aaacgccgca acggttatgc gtgccggtgc caatatggtc aaaattgagg
     7741 gcggtgagtg gctggtcgaa accgtaaaaa tgctgaccga acgtgccgtt cctgtatgtg
     7801 gtcacttagg tttaacccca cagtcagtga atattttcgg tggctacaaa gttcaggggc
     7861 gcggcgatga agcgggcgat caactgctca gcgatgcatt agccttagaa gccgctgggg
     7921 cacagctgct ggtgctggaa tgcgtgccgg ttgaactggc aaaacgtatt accgaagcac
     7981 tggcgatccc ggttattggc attggcgcag gcaacgtcac tgacgggcag atcctcgtga
     8041 tgcacgacgc cttcggcatt accggcggtc acattcctaa attcgctaaa aatttcctcg
     8101 ccgaaacggg cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg
     8161 tttatccggg cgaagaacac agtttccatt aaggagtcac gttgtgttaa ttatcgaaac
     8221 cctgccgctg ctgcgtcagc aaattcgccg cctgcgtatg gaaggcaagc gcgtggcgct
     8281 ggtgcctact atgggtaatc tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc
     8341 ccgcgccgat gtggtcgtcg tcagtatttt cgttaacccg atgcagttcg accgcccgga
     8401 agatctggca cgttatccac ggaccttgca ggaggactgc gagaagctaa acaaacgtaa
     8461 agtggattta gttttcgccc cttcggtaaa agagatctac ccgaacggta ctgaaaccca
     8521 tacttacgtt gacgttcccg ggctttcgac catgctggaa ggcgcgagcc gtccggggca
     8581 ttttcgcggc gtttcgacta tcgtcagcaa gctgtttaac ctggtccagc cggacatcgc
     8641 ctgcttcggt gaaaaggact ttcagcaact ggcgctgatc cgcaaaatgg ttgccgatat
     8701 gggcttcgat attgagattg tcggtgtgcc aattatgcgc gccaaagacg gtctggcgct
     8761 aagttcccgt aacggttatc tgacggcgga acaacgcaaa attgcgcctg gtctgtacaa
     8821 agttttaagt tcgattgctg acaaattgca ggctggggaa cgggatctcg atgaaattat
     8881 taccattgcg gggcaagaac tgaatgaaaa aggcttccgc gccgatgata ttcagattcg
     8941 cgatgccgac acattgctgg aagtttctga aaccagcaaa cgggcagtaa ttctggtagc
     9001 cgcctggctt ggcgatgctc gcctgatcga caacaaaatg gtcgagctgg cgtaatactt
     9061 aactggcgct acggctgatg gcgccagtta ttaatttacc ccacgtcatc tgtcataaca
     9121 caggtaactt aaataaggtt aattacctta tcaatctcag caagagataa atttgtcatc
     9181 tctgcaacgt ctttccgaga cattccttta cttagaagac gcagggcgaa ttcttgcctt
     9241 tcctcctgtc ttccctgctg aatccccttc tcaatccctt tctcttcaaa ccactgcgcc
     9301 agcgtcatca tagactcccc tcccgtttcc ctgtctctca gcacaccata aaacaaatcc
     9361 gcttgttcag tatgaccgcg ttgcagcata tagttttgca tggcaactaa ctgacttccg
     9421 ctagtgtacc cttcgtcaat cagcgtgacc agttgctcca gcaataacat taagtcgcgc
     9481 tggcgaatat gtttttgcag tagttcgaga atcgcaatcc gccgatgttg catgatttcg
     9541 tcatccggtg tgatggtgat atccaccagc gggaaaggac tgttatagac gcgtcgtgcc
     9601 agctccggcg agtaaaatat atcaaaccag cacattgaga gcggataagg tgtggcctcg
     9661 ccctgataaa acagtatcgg caccaccagc ggcagcttat cgtgatccgc ctccagatgc
     9721 cggtgcatgg cagcaataga ataacgcatc atgcgaaagg ccattttctt atccggcttg
     9781 ctttggtgtt caatcacaac atgcagataa ccgggattgc cctgcatttg cacggaatag
     9841 agcacgtccg tgctgtgtcc tttcaggctc tcttcaatga aactccccga ctctaaatga
     9901 agcgtgttga ggtcacaaag ttctcgtaat tccactggca aatgtatctc cagaaagtcg
     9961 cgagccgtct ccgcatgcat taaaaattgt ttaaataccg cgtcatgcgg tgtggtactc
    10021 ggtgcatcca tcttccctgt cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg
    10081 cgcgccgtcg tcagccgcct ctttagtgtt taccgagcag cgttcagaga aatttcaatt
    10141 cgttacaggc gatacatggc acgcttcggc gcggtacgca aactgctgac gcaaatgcca
    10201 tcagatgagt agacactcat caaaaatcgg gcaatactgc gtgagaattt cctcaaagca
    10261 ggcaccgcct gcttcgttaa cgacagggta gaaaggtaga agttatgatt cgcacgatgc
    10321 tgcagggcaa actccaccgc gtgaaagtga ctcatgcgga cctgcactat gaaggttctt
    10381 gcgccattga ccaggatttt cttgacgcag ccggtattct cgaaaacgaa gccatcgata
    10441 tctggaatgt caccaacggc aagcgtttct ccacttatgc catcgcggca gaacgcggtt
    10501 cgagaattat ttctgttaac ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca
    10561 tcatcgccag cttcgttacc atgccagatg aagaagctcg cacctggcga cccaatgtcg
    10621 cctattttga aggcgacaat gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg
    10681 cttgattgtt atccctgcgg ctggttactc accagccgcg acatcgtctc cagcgaatcc
    10741 gttcttaaga tataaagtcg ttttagtaac aacggattat cccccggttt tactttgcct
    10801 ttcatggttg tcaccgccag gtgaaatccg gcatcgtttg ctgccttcac ggctttgtca
    10861 ttaaatccgc caaacggata cgaaagatac cagacatgcg gattaaattg cgccagagcg
    10921 cggcgtgaac gtgcaaaatc aaacagaata ttgtgctcac tacggctcag taatatgggt
    10981 cggcgatagc catccacccg atgcaaaaaa tgggtatgtg actgaaaatc aaaaacatcg
    11041 cgaatttcgt taagctcaga aacgctcata aattgcagcg attttgggtt ccacttctgc
    11101 gggtgacgtt tgatgcgcga ggtaacaaca aacgccgtcg ccttcatgcc atattgtttc
    11161 aatacaggat acgcatagcg gctcaccgac ttgaggccat catcaaaggt aatcaccacc
    11221 gctcgcgcag ggagattgat cttattcttc acgtaacctt ccagctgcgc catgctcagt
    11281 gtcgcgtatc ccctgtcgcg cagccaggcc atttggttat tgaaagcgcg taccgaggtg
    11341 gtcgtcgaag tatggcgaaa acgggtgttt tcttcatcgc gcagaatatg gtgataggtt
    11401 agcaccggca ggccattatc gggttgggca tccagcgcgc tgatataggc gagtcgatcg
    11461 ccaatacgga tctgatacca ggtttgattt aacctgtctt tcagtttatg caaaatcggg
    11521 tagcgcaaat tgtccgccag taccccaaat ggcgcactgc ccgcactcgg cgcgttatag
    11581 accggcgtat ctttccaggt aattaagttc tgattactca gcggcttgtt gaggtcaccc
    11641 aaaccgtctt caactttttg tcgcccctga actggctcga gatgcccttt atcgataaac
    11701 cctttaccaa agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg
    11761 atttgcccgg cccgaatatt ccccacggtc accatcttgt cgccaatttg cgcccagatc
    11821 gcagcatttt cgatggtttg catataacgg gcaggtaacg cggcactgac acttgcggta
    11881 aacagcatca gcaggagaat aacagcttgt ttgtacatga aaaaataacc gagggtgaaa
    11941 caacgctttt attgtagcaa aagcgctata agaatcgtgg gttaatactc atacaaatta
    12001 tgtttgagga cgaaaggcgg atttttttgt tgttggtgcc aaagactact cacctccggc
    12061 ccacccagtt cgacaatacg ctcgcgaaac tctgaactgg tcatggtttc acggcaagcc
    12121 atcatctgtt caattaacgg taacgtgaca ccagaaatca cttcacaacg ggagtgttta
    12181 tggcttaata atgaagccac gcggtacggt ggcgctccgg ctaaatcggt taagaagatg
    12241 acaccctcac cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct gagcatatta
    12301 gagcttaatc cgcgccagaa attcacggcc cggcactgaa gaagtgcccc atgttttttc
    12361 tccagcgcat ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac
    12421 atcgccttcc tccttagccg aaaatcagtc taccctgacc attgttgata aagggtgata
    12481 gccgtcaaaa ctcctctccc ccgaatccag aggagaggaa atagccttag ctacgcaaac
    12541 cacgtccacg ttggatcagc gaccaacaga tcaaataaaa cgccacaata aagaccacca
    12601 gtacgccaaa ggtagtgacc agcggcacat catttatacc gaggaagccg tagcggaaac
    12661 cactgatcat ataaacgatt gggttcaggt gcgacagccc ttgccagaac ggcggcaaca
    12721 aagtcaggga gtaaaacacc ccgcccaaat acgtgagtgg cgttaacaca aaggttggca
    12781 ccaggctgat gtcatcgaac gttttggcaa acacgccgtt cagcaaaccc gcaagggaga
    12841 atagcaccgc cgtgagcacc agggttaagg caacgaatac ccacgaatgc acctgaaacg
    12901 gcacaaaaaa cagtgaaatt gccgtcacca gaatgccaac aaacagacca cgcgccacgc
    12961 caccgccgac atatccggca ataatgacgt gagtcggaac cggcgctacc agcagctctt
    13021 caatattacg ctggaacttg gcaccaaaaa atgatgacgc gacgttagcg taggcattgg
    13081 tgatcaccga catcatgatc agccccggta cgatgaactg catatagctg aagccatgca
    13141 tatcgccaat acgcgaacct atcaggttac cgaagataat aaagtaaagg gtcatggtga
    13201 tgactggcgg caccagcgtc tgcacccaga tacgcataaa gcgatggatc tctttcgccc
    13261 agatgctttt tagcgccacc cagtaaagat gcatcatgcg cgatctcctt gtttttcatt
    13321 aaccagtgaa acaaacagct cttccagacg gttagctttg ttacgcatac ttaatacctg
    13381 aatgccctgc tcacttaact gcgtaaatac gctgttgatc ccctgctcac gcagcacttc
    13441 aacttccagc gtcgcggtat cgaccagtcg atactgatag ccatcgagct tcggtaacgg
    13501 acttttcggt gcgagatcga gaataaaggt ttccgatttc agcttagcca gcagcgcctt
    13561 catcgaggta ttttccacca gctcaccgtg ttgaataatg ccgatattgc ggcacagcat
    13621 ttctgcttct tccaggtagt gtgtggtgag aatgatggtg gtgcctttgt cgtttaaatc
    13681 cttcaaaaag ccccacattg agcggcgaag ttcaatatcc acgcctgcag tcggctcgtc
    13741 gagaatcagt agtttaggtt catgcattaa cgcacgggca atcattaaac ggcgcttcat
    13801 cccgccagat aacatgcgcg cacgttcgtt gcgttttccc cataaatcga gctgcttaag
    13861 atatttttcg ctgcggatgt acgcctcttt gcgctccaca ccgtagtacc ctgcctgatt
    13921 caccacaatt tgctgcacgg tttcaaacgg gttgaagtta aattcctgcg gcaccagtcc
    13981 caactgacgt ttagcgttca cgacatcttt ctcgagatcg taaccaaata cgctgacccg
    14041 cccggaggtt ttatttacca gagagctgat aataccgata gtggtcgatt tcccggcccc
    14101 gttcggcccg agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcta ttccacgaag
    14161 cgcctgaacg ccgcctggat aggttttttt aagctgttga agttccagtg caatggtcat
    14221 aaatttttac ttaccttacg ttcttacact ttatctgtgg tttaaatcgt cccggagttg
    14281 ccctatatta gccaaacgta attatttggt tacaggtcgt taacctccat gaaagacata
    14341 gatacactca tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg
    14401 ttttttgaga aactggcaca agcgcaaaaa ccgcgctttc tatggattgg atgttccgac
    14461 agtcgcgttc ctgcagaacg tttaaccggt cttgagccgg gcgaactctt tgttcaccgt
    14521 aatgttgcta acctggtcat tcacaccgac ctgaactgcc tttccgtggt tcagtatgca
    14581 gtggatgtac tcgaagttga acacattatt atctgtggcc actacggttg cggcggcgta
    14641 caagccgcag ttgaaaaccc ggaactgggg cttatcaaca actggctgct gcatatccgc
    14701 gatatctggt tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat
    14761 accttgtgtg aactgaacgt catggaacag gtttataacc tgggccactc caccattatg
    14821 caatcagcgt ggaaacgcgg gcagaaagtt accattcacg gctgggccta cggcattcac
    14881 gacggcttgc tgcgtgatct ggatgtcaca gcgaccaacc gcgaaaccct tgagcaacgt
    14941 taccgtcacg ggatttccaa cctcaagctg aaacacgcca accacaaata aaaatgccat
    15001 gccggatgca acacatccgg caacttcaca cttactcgtc cagcagaatc actttgccga
    15061 tatacggcag atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat
    15121 ccgggatcga gaaaccgata aattcaaccg ggacgttcac ttcacgacgg gacggtttat
    15181 ccagcagcgt acaaatcgcc aacgacttcg gttcgcgcag gcttaagatc tcacgcactt
    15241 tcgacagtgt attccccgag tcgatgatat cttcaacaat cagcacgtcc ttgccacgga
    15301 tatcttcatc cagatctttg aggattttca catcacgggt ggtggacatg ccgctaccgt
    15361 agctggaggc ggtcataaag tcgacttcat gagatacctg aacttcacgg cacagatccg
    15421 ccataaacat aaatgagcca cgcagcagac ccaccagcac catatcgctg ccgctgtctt
    15481 tgtaacgctc agtaatctga cgacccagtt cggcgatacg cgctttaatc tccgcttcgg
    15541 ggatcattac ttctacagta tgtttcatat ctctaaccat atgatttaaa aacaaatcac
    15601 ttcgcacggg caggttttgg caccgcaaca aagcgcaaga cacgcagtat accagtaaaa
    15661 ctattcattc gcggaatctg ttaataaagc t
//
LOCUS       DAZFRN010000116        15655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFRN010000116 DAZFRN010000000
VERSION     DAZFRN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.118"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            114..1478
                     /locus_tag="ACHKUB_003533"
     CDS             114..1478
                     /locus_tag="ACHKUB_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6656500.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLTVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1606..2097)
                     /gene="yajQ"
                     /locus_tag="ACHKUB_003534"
     CDS             complement(1606..2097)
                     /gene="yajQ"
                     /locus_tag="ACHKUB_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO6656501.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2265..3176
                     /gene="panE"
                     /locus_tag="ACHKUB_003535"
     CDS             2265..3176
                     /gene="panE"
                     /locus_tag="ACHKUB_003535"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO6656502.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3139..3729
                     /gene="yajL"
                     /locus_tag="ACHKUB_003536"
     CDS             3139..3729
                     /gene="yajL"
                     /locus_tag="ACHKUB_003536"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO6656503.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3783..5231)
                     /gene="thiI"
                     /locus_tag="ACHKUB_003537"
     CDS             complement(3783..5231)
                     /gene="thiI"
                     /locus_tag="ACHKUB_003537"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO6656504.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5437..5679
                     /gene="xseB"
                     /locus_tag="ACHKUB_003538"
     CDS             5437..5679
                     /gene="xseB"
                     /locus_tag="ACHKUB_003538"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO6656505.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5679..6578
                     /gene="ispA"
                     /locus_tag="ACHKUB_003539"
     CDS             5679..6578
                     /gene="ispA"
                     /locus_tag="ACHKUB_003539"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO6656506.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6603..8465
                     /gene="dxs"
                     /locus_tag="ACHKUB_003540"
     CDS             6603..8465
                     /gene="dxs"
                     /locus_tag="ACHKUB_003540"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO6656507.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8520..9494
                     /gene="yajO"
                     /locus_tag="ACHKUB_003541"
     CDS             8520..9494
                     /gene="yajO"
                     /locus_tag="ACHKUB_003541"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO6656508.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAGRLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(9548..10066)
                     /gene="pgpA"
                     /locus_tag="ACHKUB_003542"
     CDS             complement(9548..10066)
                     /gene="pgpA"
                     /locus_tag="ACHKUB_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO6656509.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10044..11021)
                     /gene="thiL"
                     /locus_tag="ACHKUB_003543"
     CDS             complement(10044..11021)
                     /gene="thiL"
                     /locus_tag="ACHKUB_003543"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO6656510.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11099..11518)
                     /gene="nusB"
                     /locus_tag="ACHKUB_003544"
     CDS             complement(11099..11518)
                     /gene="nusB"
                     /locus_tag="ACHKUB_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO6656511.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11538..12008)
                     /gene="ribE"
                     /locus_tag="ACHKUB_003545"
     CDS             complement(11538..12008)
                     /gene="ribE"
                     /locus_tag="ACHKUB_003545"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO6656512.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12097..13200)
                     /gene="ribD"
                     /locus_tag="ACHKUB_003546"
     CDS             complement(12097..13200)
                     /gene="ribD"
                     /locus_tag="ACHKUB_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO6656513.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPTLTVRWSELDEQTQAL
                     YPQQNLRLPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13204..13653)
                     /gene="nrdR"
                     /locus_tag="ACHKUB_003547"
     CDS             complement(13204..13653)
                     /gene="nrdR"
                     /locus_tag="ACHKUB_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO6656514.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13804..14343
                     /locus_tag="ACHKUB_003548"
     CDS             13804..14343
                     /locus_tag="ACHKUB_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6656515.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14642..15526
                     /gene="tsx"
                     /locus_tag="ACHKUB_003549"
     CDS             14642..15526
                     /gene="tsx"
                     /locus_tag="ACHKUB_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO6656516.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
BASE COUNT         3776 a         4137 c         4066 g         3676 t
ORIGIN      
        1 aaacatacct gacacacaaa ttactctcct ctgtaatatt ttctcattaa cactctgttt
       61 acccaacatg cgctccgcac tacactatgt gggcattttt ttaaggggtg gtaatgaacg
      121 attataaaat gacgccaggt gagaggcgcg cgacctgggg tttagggacc gtattctcgt
      181 tgcgcatgct gggcatgttt atggttctgc cggttctgac cacgtacggc atggctctgc
      241 aaggtgccag cgaagcatta atcggtattg ccattggtat ttatggtctg actcaggccg
      301 tttttcagat tccgtttggc ctgctttccg accgcattgg tcgcaaacca ttaattgtcg
      361 gtgggctggc ggtgtttgcc gccggtagcg ttatcgctgc gctttccgac tccatctggg
      421 gaattattct gggccgggcg ctacaaggct ctggagcaat tgccgccgcc gttatggcgc
      481 tgctttccga tctcacgcgc gaacaaaacc gcaccaaagc gatggcgttt atcggcgtga
      541 gctttggcat tacctttgct attgcgatgg tgcttggccc gatcatcact cacaaacttg
      601 ggctgcacgc gctgttctgg atgatcgcta ttctggcaac gaccggcatt gcgttgacca
      661 tttgggttgt gcccaacagt agcactcacg tacttaatcg tgagtccgga atggtgaaag
      721 gcagtttcag taaagtgctg gcggaaccgc ggctgctgaa actcaacttt ggcattatgt
      781 gtctgcatat tttgctgatg tcgacgtttg ttgccctgcc cggacaactg gctgatgcag
      841 ggttcccggc ggctgaacac tggaaggtct atctggcgac aatgctaatc gcctttggct
      901 cggtcgtgcc tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa gtctttgtct
      961 tctgcgtcgg gttgatcgtg gttgcggaaa ttgtgttgtg gaacgcgcaa acgcagttct
     1021 ggcaactggt ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg gaagccctcc
     1081 tgccctcact tatcagtaaa gagtcgccag caggttacaa aggtacggcg atgggtgttt
     1141 actccaccag ccagtttctt ggcgtggcga ttggcggttc gctgggcggc tggattgacg
     1201 gcatgtttga cggtcagggg gtatttctcg ctggcgcaat gctggccgca gtgtggctga
     1261 cagtcgccag taccatgaaa gaaccgccgt atgtcagcag tttgcgcatt gaaatcccgg
     1321 cgaacattgc cgcaaacgag gcgttaaaag tgcgtttgct agaaactgaa ggcatcaaag
     1381 aagtgttgat tgcagaagaa gaacattcag cttatgtgaa aatcgacagc aaagtgacga
     1441 atcgctttga gatagaacag gcaattcgtc aggcataaaa aacgcggcga attgcctgct
     1501 gcgctacgct tatcaggcct acaaagtttc tgaattacaa acctttgtag gccgaatcag
     1561 gcgttcatgc cgcatctgac atgaacaaaa cgcacatagt cgcgattaat cgcggaagtt
     1621 tttgaactgg aacggctgac cgagatcgcc accacgtacc atcgccatga cagcctgcaa
     1681 atcatcacga gatttgcccg ttacgcggat ttcatcgccc tgaatttgcg cctgcacttt
     1741 cagtttgctg tctttgatca tcttgacgat tttcttctgg gtcgcgctct caatgccctg
     1801 tttcagtttc gcttccacaa accaggtttt accgctatga acgatatttt ccggtacatc
     1861 cagtgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat ccagcaactg
     1921 attgacctgg aagtcggact cgctcaacac tttgatggtt ttgctggcgt cgttcagctc
     1981 aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcgac tcgcgttatc
     2041 gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag atggcatctt
     2101 ttcctctccc ttcatttttg atgcgaagca taatacctgc aaagttaaat aaccaacagt
     2161 cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac aattaaggga
     2221 tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa attaccgtat
     2281 tgggatgcgg tgccttaggg caattatggc ttacagcact ttgtaaacag ggtcatgaag
     2341 ttcagggctg gctgcgcgta ccgcaacctt attgtagtgt gaatctggtt gagacagatg
     2401 gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttctcgcc accagcgatc
     2461 tgctcctggt gacgctgaaa gcatggcagg tttccgatgc cgtcaaaagc ctcgcatcca
     2521 cactgcctgt agctacgcca atactgttaa ttcacaacgg catgggcacc atcgaagaat
     2581 tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc cgccgcgacg
     2641 gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat cggtccggca cggcaacagg
     2701 atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac gttgcctggc
     2761 ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgtgtg attaatccac
     2821 tgaccgccat ctggaattgc ccgaatggtg aattacgtca tcatccgcaa gaaattatgc
     2881 agatatgcga agaagtcgcg gcagtgatcg agcgcgaagg gcatcatact tcagcagaag
     2941 atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc tcgtcgatgt
     3001 tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt tttctcttac
     3061 gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt gaaatggtaa
     3121 aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa
     3181 gagactgaag ccgtcaccac tatcgatctg ctggttcgcg gcggtatcaa agtcaccact
     3241 gccagcgtcg ccagcgatgg caacctggcc attacctgct cgcgcggcgt gaagctgctg
     3301 gcggatgcgc cgctggtcga ggtggctgat ggcgaatatg acgtgatcgt actgcctggt
     3361 ggcattaaag gcgcagagtg ttttcgcgat agtaccctgc tggttgaaac cgttaaacag
     3421 ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac cgtgctggtg
     3481 ccgcacgata tcttcccgat tggtaatatg accggcttcc cgacgctgaa agacaaaatt
     3541 cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa attgctgacc
     3601 agccaggggc cggggacagc tatcgacttt ggtctgaaaa ttatcgacct gctggttggg
     3661 cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat ttataattat
     3721 tacgagtagt gtcggatgcg gtaagcgtcg catctgacca gatgcgacgt tacaaccaca
     3781 aattacgggc gatatacctt cacattgtta aagccctgct cgcgcagata gagcgcctgc
     3841 agacggctca tcaccccgcg ctcacaccac agtagccagg ttctgttctg gtcgagatcg
     3901 ccaaacttgg tgctcagttt atagaacggc agagaaacta catcaatccc ttcgactttc
     3961 agtggcttat cttcctgttc atcgatagaa cggatatcga ggatcacgtc gttcgggccg
     4021 aagccattga cggtttccac ttccaccact tcctgctcgg tctgctgggc gatttcgcgg
     4081 atatcaacgt tattcgcttc ttcaaccact ttatcgagaa tgctgaagtc gaatttctct
     4141 tcttccgctt caatcttcga cttaaccgct ttcaccgtcg ggcttttgga gataacaccg
     4201 caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc caggttgatg
     4261 atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtatcgga gacgttatca
     4321 atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc gccggtgacc
     4381 agcgcctgta cgccgtaacg ttcagccact ttagacgcgg cacgcaccat catacgtttg
     4441 aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac gaccggttcg
     4501 aaattaatag cgacaaaacg cacgcggtgg gagctgccaa aacggttcca cagataatgc
     4561 gccacctgac gcacgccaat ttcatgcgcc gcgccgccaa ggttaaagaa acagtaatgc
     4621 acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa accaccggaa
     4681 atgagcgaca gcacatcttc ctgggtgccg atcgggaaac cgccaatacc ttcgtagcgg
     4741 cctttaatca gcaggagacg atcgtcttcc acttccagat ggacagtcac ttccggattg
     4801 gtcagcttca cgcgcgcgga ctcaatatgc tgatttaaac cgccgccgac gtaacgttcc
     4861 acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca gaaggttttg
     4921 ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg catgtcggta
     4981 aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt cagggcgtcg
     5041 cgaatagcca gacgctggtt ttcatctttt gcgcgaactt cgatgttatc ccagtggcgg
     5101 acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc ggtaaggatt
     5161 tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa caatttaatg
     5221 ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta tagggaaaaa
     5281 tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta acatcgcttt
     5341 gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt ccgttaccat
     5401 gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa tgaggctccc
     5461 gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct ggaaagtggc
     5521 gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag
     5581 gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc tgacaatgaa
     5641 gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg cagcaactcg
     5701 aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca ctgccctttc
     5761 agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt aagcgcctgc
     5821 gaccttttct ggtttatgcc accggtcata tgttcggcgt tagcacaaac acgctggacg
     5881 cacccgctgc cgccgtagag tgtatccacg cttactcatt aattcatgat gatttaccgg
     5941 cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag tttggcgaag
     6001 caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt ctaagcgatg
     6061 ctgatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa ctggcgagcg
     6121 ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg gaaggcaaac
     6181 acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca ttgattcgcg
     6241 ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct ctgccagtac
     6301 tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac atcctggatg
     6361 tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa cttggtaaaa
     6421 gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg gatctgatcg
     6481 acgatgcccg tcagtcgctg aaacaactgg ctgaacagtc actcgatacc tcggcactgg
     6541 aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt aataggcccc
     6601 tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc acccaggagt
     6661 tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc cgctatttac
     6721 tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg gtcgaactga
     6781 ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg gatgtggggc
     6841 atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc accatccgtc
     6901 agaaaggcgg tctgcatccg ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg
     6961 tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct gccgagaaag
     7021 aaggcaaaaa tcgccgcacc gtctgtgtca ttggcgatgg cgcgattacc gctggcatgg
     7081 cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg gtcctcaacg
     7141 acaatgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa taaccatctg gcacagctgc
     7201 tttccggtaa gctttactct tcgctgcgcg aaggcgggaa aaaagttttc tctggcgttc
     7261 cgccaattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg gtagtgcctg
     7321 gcacgttgtt tgaagagctg ggctttaact acatcggccc ggttgacggt cacgatgtgc
     7381 tggggcttat caccacgctg aagaacatgc gcgacctgaa aggcccgcag ttcctgcata
     7441 tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agaccccatc actttccacg
     7501 ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc ggtttgccga
     7561 gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac aacaagctga
     7621 tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc
     7681 cggatcgtta cttcgacgtg gcaatcgccg agcaacacgc ggtgaccttt gccgccggtc
     7741 tggcgattgg tgggtacaaa cccattgtcg cgatttactc cactttcctg caacgcgcct
     7801 atgatcaggt gctgcatgac gtggcgattc aaaagctccc ggtcctgttc gccatcgacc
     7861 gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg cgcttttgac ctctcttacc
     7921 tgcgctgtat accggaaatg gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga
     7981 tgctctatac cggctatcac tataacgacg gcccgtccgc ggtgcgctac ccgcgcggta
     8041 acgcggttgg cgtggaactg acgccgctgg aaaaactgcc aattggcaaa ggcattgtga
     8101 agcgtcgtgg cgagaaactg gcgatcctta actttggtac gctgatgcca gacgcggcga
     8161 aagtcgctga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa ccgcttgatg
     8221 aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta gaagaaaacg
     8281 ccattatggg cggcgcaggc agcggcgtga acgaagtgct aatggcccat cgtaaaccag
     8341 tacccgtgct gaacattggc ctgcctgact tctttattcc acaaggaact caggaagaaa
     8401 tgcgcgccga actcggcctc gatgccgccg gtatggaagc caaaatcaag gcctggctgg
     8461 cataatccct actccactcc tgctatgctt aagaaattat tcatggcagg agtggcagca
     8521 tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc ctcggctgta
     8581 tgacctttgg cgagccagat cgtggtaatc acgcatggac actgccggaa gagagtagcc
     8641 gtcccatcat taaacgcgcg ctggaaggcg gcataaattt ctttgatacc gccaatagct
     8701 attccgatgg tagcagcgaa gagatcgttg gtcgcgcact gcgggatttc gcccgtcgtg
     8761 aagacgtggt cgttgccacc aaagtgttcc atcgcgttgg tgatttaccg gaaggattat
     8821 cccgtgcaca aattttgcgc tctatcgacg acagcctgcg acgtctcggc atggattatg
     8881 tcgatatcct gcaaattcat cgctgggatt acaacacgcc gatcgaagag acgctggaag
     8941 ccctgaacga cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca tccatgcacg
     9001 cttcgcagtt tgctcaggcc ctgaagctac aaaaacagca cggctgggcg cagtttgtca
     9061 gtatgcagga tcactacaat ctgatttatc gcgaagaaga gcgcgagatg ctgccactgt
     9121 gttatcagga gggcgtggcg gtgattccgt ggagcccgct ggcgcggggg cgactgacgc
     9181 gtccgtgggg agaaactacc gcacgactgg tgtctgatga ggtggggaaa aatctctata
     9241 aagaaagcga tgaaaatgac gcgcagatcg cagggcggtt aacaggcgtc agcgaagaac
     9301 tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt gagtaaaccg ggcattgccg
     9361 caccgattat cggaacttcg cgcgaagaac agcttgatga gctattgaac gcggtggata
     9421 tcactttgaa gccggaacag attgccgaac tggaaacgcc gtataaaccg catccggtcg
     9481 taggatttaa ataagagtcc aggcctgatg agacgcaaca agcgtcacat caggcactgg
     9541 tgcacaacta cgacagaata cccagcggcc agtgatgacc gataaagtac aggatacctg
     9601 cggagatcac cccggcgaca atatcatcga tcatgatccc catgccgcca tgtacattgc
     9661 gatcaaacca gcggatcggc cacggcttcc acatatccag aatacggaaa atgacgaatc
     9721 cggcagtgac ccactgccag tcattggtcg gcaatgccat gagcgtgatc cacatgccga
     9781 taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc catatctttc gccgtttgat
     9841 gacaaagata gacgccgata cagatcccca acatcaccac cagcgagtag agctgccagg
     9901 gtaaaaaggt catcagatac cagaaaggga tcgccgccag cgatcccatc gtgccaggaa
     9961 cgatcgggct taatccactg ccgaatccga cagcaagcag atgccacgga ttactcatct
    10021 tcaggcgact tttcgcgaca tctttatggc gtggcaaaat ggtcatatcc tttccagtca
    10081 aaggtgacag gttcgccgtc acggataaaa caaagccctt cgatatcggc ggtcatctgc
    10141 ccgatacagg taaacggtac gcccaggtgt ccgagagcca catccagcgc gccacggttc
    10201 agttccggca cagtgaaaca caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc
    10261 gcctgttcag gttcaacatg gcgagaaagc gcatcagaaa acggcagcaa tgccaggtca
    10321 atacgtgcgc cgcagtcgct ggctttcacg atatgcccga gatcggaaat cagaccgtca
    10381 gagagatcga tagctgaatt tgccagatcg cgcagtgcct gtccctgtaa aatacgcggc
    10441 gatggacgga gatgacgttt gatcaagtag tccgcatctt tagcatcggc aacctgcaaa
    10501 cggttttgca aaatcgccag cccggcggcg ctatcgcccg gtgtaccggt cacatagatc
    10561 cagtcacccg gtttcgctcc agagcgcgtt aaggctcgtc ccatcggaac aaagccgtgg
    10621 atacccaacg tcattgataa tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg
    10681 taataattga gaagatcaaa caaactgtcg ctgaaggact caagccacgc ttcgtctacg
    10741 tccggtaagg ttaatgccag cgtcagccag gccggatcgg cccccatcgc tgccagatcg
    10801 cttaggttca ccgccagtgc tttatacgcc agatcagcag gatcgatatc atggaggaag
    10861 tggttgcccg ccaccagcgt atcagtgctg atcgccaggg tctgtttctc ggggatattg
    10921 agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa gacgagaact tcttacacgg
    10981 tcaaaataac gggcaatcag ggagaactcg ccacatgcca tacgttatgc ctcagcaggt
    11041 aaaaaagaaa aggccggcga tcgcggaacg gtcttccgtg aatctaccgg cctggatatc
    11101 actttttgtt agggcgaatc acaggtgctg ctttatcgag tacgccgttg acgaacttat
    11161 ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc ttcgttaatg gccactttgt
    11221 atggcacatc gctacgttta gacagctcgt acagcgcaat gcgcagtact gctttttcta
    11281 cctgtcccag ttcttccagc aggcgggaca ggtatggctt catcagtccg tcgaggtatg
    11341 cggtgttagt cgccaccccg gccagcagct cacggaagta caggacgtca acgtctttta
    11401 catcctgttc agccaggaac tggtattcaa catcagcgat gtcgttctgg gacaactgcc
    11461 aggagtagag cgcctggacg gcacactcac gagcgcggcg acgagcagca ggtttcacgg
    11521 atttcccctt actaatttca ggccttgatg gctttcaata cattaatcat ttcaagcgcg
    11581 gtcagtgcag cttctgcacc tttgttgcca gctttggtgc cagcacgttc gatcgcttgt
    11641 tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca
    11701 tgcgccaggc cgttgcttgc accaccagcg acatattcaa agtgggcagt gccaccacga
    11761 ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg ttttagccag tgcacccgcc
    11821 gccagcggca gctcataggc accaggcacc caaacaacgg taatgttttc atcttttacc
    11881 tgaccgatac gtttcagtgc gtcaattgca ccttccagca ggctgtcatt gataaagttg
    11941 ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata
    12001 atgttcattt tcttccttcg ggttcgagta tggccccgca ggggggcgga ttttagcata
    12061 atatttcgtg cgctgcttcc ctttcgagcc gggagatcat gcacccacta aatgcaggca
    12121 aacatccggg cctacatgac gtatctcttt gaatttaaat tggggggcgt cggctaattt
    12181 ctcaagccct ggcagcgtgc ataatccgcg ggcgtcgctg cctaatagtt taggtgcgat
    12241 atagacaatc agctcatcga ctaaacccgc ctgcagcaat gcgccagcga gcgttggccc
    12301 cgcttccacc cagatgctgt taatttgctg tttacccagt tgcatcatca gtacaaccag
    12361 atccagatga cctttatgct ctggaatcag caaggtacgc accgtttccg gccactcact
    12421 agaatcttcc tgcgtacgcg cgaaccaagt ttcgccgggc tgctgcacaa tgcgatgttc
    12481 cggcgtcacg cgattttggc tatcaatcac aatacgtact ggcaggcgga gattttgttg
    12541 cgggtagagc gcctgagttt gttcatccag ttcagaccaa cgcactgtta aggttggatc
    12601 atccgccagc accgtggcgc tgctggttaa aatggcatga ctttgtgcgc gctgtcgttg
    12661 tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt tcgccgctcg ccatcgccgt
    12721 gcggccatca agcgatgcgc caagttttaa ctgaatataa ggaaagccgg tgcgcatccg
    12781 cttgagaaag cctttattca attgctcggc ttcactcatc atcagcccgt ggctgacgtc
    12841 aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt taggatcttg
    12901 catcgaggca accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc acggtggcgt
    12961 acgaccatga tggctacagg gttcgagtgt gacataggcg gtcgcacctt tggctttttc
    13021 acccgccata cgcaacgcgt gtacttcggc atgtggttca cccgcacgtt ggtggtaacc
    13081 ttcaccgaca atttcgccat ctttgacaat gacgcacccg acattcgggt tgggatgcgt
    13141 ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc atgtaatact cgtcctgcac
    13201 ggcttagtcc tccaggcgcg cgatctcttc gccaaattct ttgatatctt cgaaactgcg
    13261 gtagacagag gcaaaacgga tataggcgac tttatcgagc tttttcaatt gctccatcac
    13321 cagattgcca atcatcttgc tcggcacttc gcgctcaccg gtggcgcgca gctgcgattt
    13381 aatatgattg attgccattt cgacgtcatc ggaactcacc ggacgttttt ccagcgcccg
    13441 cagcattccg ctacgcaatt tctcttcatt aaacggttca cgcacgtcgt tgctttttac
    13501 aacacgcggc ataaccagct ccgccacttc aaaggtggtg aaacgttcat tacacaccag
    13561 acactgccga cggcggcgta cggatgaacc ctcgcccacg agacgagagt caattacctt
    13621 agtgtccacg gcgaaacaga atgggcaatg catacggcgt cctgaccagg tggttaacag
    13681 aaatctattt taccctgaac tgacgataca acaaaggcgc aacgcttttg agataaagga
    13741 tcgatgcttt cgctgctttt accctctacc attaggctaa ttgcgacgag aaagggacga
    13801 cctatgaata ctaacgtttt tcgactgctc ctgctgggaa gcctgttcag ccttagcgcc
    13861 tgtgtgcagc agagcgaagt gcgacagatg aaacacagcg tcagcacgct gaaccaggag
    13921 atgacgcaac tcaataaaga aacggtcaaa atcactcagc aaaacaggct gaatgcaaaa
    13981 tccagcagtg gggtttacct tctgcctgga gcgaaaacac cggcaagact ggaaagccag
    14041 atcggtactt tacgtatgtc gctggtgaat attacgcctg atgcagatgg caccaccctg
    14101 acgctacgta ttcagggcga gtcgaatgat cccttgcccg cattcagcgg aactgttgaa
    14161 tatgggcaaa ttcagggaac aatagacaac tttcaggaaa tcaatgtgca gaatcaattg
    14221 attaatgccc ctgccagcgt cctcgccccc agcgatgttg atattccgtt acagttaaag
    14281 ggtatttctg tagaacagtt agacttcgta cgtatccacg atattcaacc tgttatgcag
    14341 taaacgtatt tcggggcgat tttgtgcgtc ccgcaacatc tttccccgtc attttgttac
    14401 tccgcttaca tcaccctgat tgatagtaaa agtttgcaac aagggtgaaa gtcagtacaa
    14461 tccctgcccg aatgtgtgta aacgtgaacg caatcgatta cgtaaatgat agaactgtga
    14521 aacgaaacat atttttgtga gcaatgattt ttataatagg ctcctctgta tacgaaatat
    14581 ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga ttttcaaaca gtggcataca
    14641 tatgaaaaaa acattactgg cagccggtgc ggtactggcg ctctcttcgt cttttactgt
    14701 caacgcagct gaaaacgaca aaccgcagta tctttccgac tggtggcacc agagcgttaa
    14761 cgttgtcgga agctatcaca cccgtttcgg accgcagatc cgcaacgata cctaccttga
    14821 gtacgaagca ttcgctaaaa aagactggtt cgacttctat ggttatgcgg atgcgccggt
    14881 attcttcggc ggtaactccg atgcaaaagg tatctggaac cacggttctc cgctgtttat
    14941 ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat actgacctta gcttcggtcc
    15001 gttcaaagag tggtacttcg cgaacaacta catttacgac atgggtcgta ataaagatgg
    15061 tcgccagagc acctggtaca tgggtctggg taccgatatc gacactggcc tgccgatgag
    15121 cctgtccatg aacgtctatg cgaaatacca gtggcagaac tatggcgcag cgaacgaaaa
    15181 cgagtgggac ggttaccgtt tcaaaattaa atactttgtg ccgattaccg atctgtgggg
    15241 cggtcagctg agctacatcg gcttcaccaa cttcgactgg ggttccgatt taggggatga
    15301 cagcggtaac gcaatcaacg gtattaagac ccgtactaat aactctatcg cttccagcca
    15361 tattctggct ctgaactacg atcactggca ctactctgtc gtagctcgtt actggcacga
    15421 cggtggtcag tggaacgacg atgcagaact gaacttcggc aacggcaact tcaacgttcg
    15481 ctctaccggc tggggtggtt acctggtagt aggttacaac ttctgattat gaaaatgccg
    15541 ggatttattc ccggcatttc tgattgttaa caacgtgtgc tttgttcatg ccggatgcgg
    15601 cgtaaacgct ttgtccggcc cacaaaagca cgtaaattca atatattgct gaaat
//
LOCUS       DAZFRN010000117        15646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFRN010000117 DAZFRN010000000
VERSION     DAZFRN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.119"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..563)
                     /locus_tag="ACHKUB_003550"
     CDS             complement(<1..563)
                     /locus_tag="ACHKUB_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656517.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLAQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSL"
     gene            complement(1143..2156)
                     /locus_tag="ACHKUB_003551"
     CDS             complement(1143..2156)
                     /locus_tag="ACHKUB_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO6656518.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIATGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     ASAKTLPGTTYTPIEVSCCSPTAKAIEKPVVNCCNTKAVASINPIKLATKDALQQFKD
                     VLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLAS
                     VLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQ
                     LLF"
     gene            2263..2559
                     /locus_tag="ACHKUB_003552"
     CDS             2263..2559
                     /locus_tag="ACHKUB_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6656519.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2693..3118)
                     /gene="arsC"
                     /locus_tag="ACHKUB_003553"
     CDS             complement(2693..3118)
                     /gene="arsC"
                     /locus_tag="ACHKUB_003553"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO6656520.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(3131..4420)
                     /gene="arsB"
                     /locus_tag="ACHKUB_003554"
     CDS             complement(3131..4420)
                     /gene="arsB"
                     /locus_tag="ACHKUB_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO6656521.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKAGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(4474..4827)
                     /gene="arsR"
                     /locus_tag="ACHKUB_003555"
     CDS             complement(4474..4827)
                     /gene="arsR"
                     /locus_tag="ACHKUB_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO6656522.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            5567..5650
                     /gene="dinQ"
                     /locus_tag="ACHKUB_003556"
     CDS             5567..5650
                     /gene="dinQ"
                     /locus_tag="ACHKUB_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO6656523.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5704..7056)
                     /gene="gorA"
                     /locus_tag="ACHKUB_003557"
     CDS             complement(5704..7056)
                     /gene="gorA"
                     /locus_tag="ACHKUB_003557"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO6656524.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7128..7970)
                     /gene="rlmJ"
                     /locus_tag="ACHKUB_003558"
     CDS             complement(7128..7970)
                     /gene="rlmJ"
                     /locus_tag="ACHKUB_003558"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO6656525.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            8173..10215
                     /gene="prlC"
                     /locus_tag="ACHKUB_003559"
     CDS             8173..10215
                     /gene="prlC"
                     /locus_tag="ACHKUB_003559"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO6656526.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10221..10973
                     /gene="rsmJ"
                     /locus_tag="ACHKUB_003560"
     CDS             10221..10973
                     /gene="rsmJ"
                     /locus_tag="ACHKUB_003560"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO6656527.1"
                     /translation="MQICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11022..12491)
                     /gene="dtpB"
                     /locus_tag="ACHKUB_003561"
     CDS             complement(11022..12491)
                     /gene="dtpB"
                     /locus_tag="ACHKUB_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO6656528.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            12657..12710
                     /locus_tag="ACHKUB_003562"
     CDS             12657..12710
                     /locus_tag="ACHKUB_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656529.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(12809..13243)
                     /gene="uspA"
                     /locus_tag="ACHKUB_003563"
     CDS             complement(12809..13243)
                     /gene="uspA"
                     /locus_tag="ACHKUB_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO6656530.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13634..13969
                     /gene="uspB"
                     /locus_tag="ACHKUB_003564"
     CDS             13634..13969
                     /gene="uspB"
                     /locus_tag="ACHKUB_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO6656531.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14040..15539)
                     /gene="pitA"
                     /locus_tag="ACHKUB_003565"
     CDS             complement(14040..15539)
                     /gene="pitA"
                     /locus_tag="ACHKUB_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO6656532.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT         4028 a         3902 c         3995 g         3721 t
ORIGIN      
        1 tacaggcttt gaatatcttt tatattagtt ggtaagtcag ctcgttgttc agcaataaaa
       61 ttagtatctg aaaacatggt ttggacactg aatgttatgg gtattccctc ttctacgcta
      121 attttagtta tgattgcctt gtcgggttta ggattagcga caacaatgat tcgacattca
      181 gcacttttgt ttttttcata tctaatgtta ttaatctgat taagtattga ttgtgtctgt
      241 tgcgtaagtt gtgtcgaact tatcgtttca atgttagtaa taagacttac aagttgagcc
      301 acaacttttg agtagtctgc atttgtatat gtagtgttcg tgtggcacgc attaatttgt
      361 tgaagttggt gttgcgctaa atcatattga tcagagatat atccttctcc agagaatgtg
      421 gaattaatgg gttgttgttc ttcccaatta tcgcaaggcc acttcggtgt gtctggcgtt
      481 ttagcattat cggtatttgt tgtgcagtta taaatatcgc cgtgatatgt atttattata
      541 cctggggtaa agtcaattga cataaaattc cttattcatc atttgtttta tgctaagatg
      601 tttaaattgg ataattagaa caggaatgga ttttcttcta cacacaataa atataatgcg
      661 ttatatcttc ctgatataat tacctttaaa ttccctttca tttccacccg gtaatatttg
      721 tgtataaaaa aaggcagtga tagtatcgta aaggaaaacg ttaacaacta tcaaaaagcg
      781 ctcattttaa aaagttattt tgttctccga tagtgtgtga agtaatattt ttgtgatgtt
      841 ttagatgtgt aataatgaac caggttaaag tgtatttaaa aatgtgttgt attagcgact
      901 gaaatctgcc ttttaaaaag gaggagggta ctgagacaaa gagtcattgc ttctccgata
      961 aaatgtcaga gaagcatgat tacgtggtta ttgtctgttg atgacttgtt gaccgagccg
     1021 tcatcattct caatgcatta gttaaatcat ttgtgatttt taaggattat cctgtctatg
     1081 attttctact tagaaggata tggcactaac tatgcatctt atagtttact gctggcatct
     1141 atttaaaata gcaattgagt caaatagccc atcaagatag ccatacctaa tataaccgtc
     1201 aggaatgcag ctatcatcgg cattctgaac attgatttaa gtaatatcac ttccgtcagg
     1261 cttgcgccgg cactgccgat gattaatgcc attaatgctc ccagacccat tccttttgtc
     1321 atcagaacag atgccagagg gataaccgcc tcagcccgga tatacagcgg aataccgaca
     1381 acggcgctta atgggatggc gaagggatta tctgctcctg catgagcggc aatccacgct
     1441 gagggaataa agccataaat aaaagagcct attaataccc ctaacaaaag atatggcagt
     1501 acatctttaa actgttgcaa cgcatctttg gtggctagtt ttatgggatt aatacttgcc
     1561 actgctttgg tattgcaaca gttaacgacg ggtttctcaa tggctttagc cgttggacta
     1621 cagcaactca cttctattgg cgtatatgtc gtccccggca aagtttttgc cgacgctttg
     1681 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
     1741 agagaatcca ggataatact ggcaagaacg gagacgccgg tggcgataat cgcgtacaac
     1801 aaggtgactt tccaaccaaa ggtcacccac attagtccga cgataatggg attaagtaat
     1861 ggtgaaacaa ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca
     1921 cgtagcatgg gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc
     1981 aggagatagc ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc
     2041 ttttgtctta tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg
     2101 gcgagaaaca caaacatctc tgcggcatct tgcagcatcg caagccaact actcatacgt
     2161 aacctcattg gataaatcta tatttccaga ataatcgaaa tgaaattcag atcaagatat
     2221 ttctggtatt atcgaaatat caaccagaca gaggggtgtc atatgcagtt ggaagaagtc
     2281 gctaaagcgc tgaaagagct aggtcacccg acccgtttgt tcatatttaa acatcttgtc
     2341 aaagccgggg aacaagggtt gccggtcggt gagttgcaaa agcagcttgg gatacctggc
     2401 tccacactca gccaccatat atcagcactg gtatcggtag gactggtcaa acaaaaccgt
     2461 gagagtcgga cgctgatgtg tgtttcgcaa tacgcaatgc tggaagcgat aattgaattc
     2521 ttacgcgagg agtgctgcgt aaatagcaaa attgcctgat gcgctacgct ctacatattt
     2581 catgccatat attgaatttg cacggttttg taggtcggat aagacgcgtt agcgtcgcat
     2641 ccgacataaa caaagcgcac tttaccaact atgggcagtg gatatcgccg ctttatttca
     2701 accgcttacc cgcttcatca accactttct cgccatcttc cttggagaat gcgccttttt
     2761 gcgcatctgg cagaatttcc agcaccactt ctgaagggcg gcacaggcga gttcccagcg
     2821 gcgtcaccac aatcgggcga ttaatcagaa tcgggtgctg aagcataaag tcgattaacc
     2881 gatcgtcagt aaatttatct tctgcaaggc ccagttcctc atacggctcg acgtttttac
     2941 gcaacagcgc gcgtacggaa atccccatat cggcaatgag tttgaccagc tcatcgcgcg
     3001 ttggcggtgt ttccagataa tagataatgg tcggttctgt gccgctgttg cggatcatct
     3061 ccagcgtatt acgcgacgtg ccgcaggccg ggttgtgata aatggtaatg ttgctcatat
     3121 cagtatctca ttacaaagtg aaagagagac gtagcgccag cgcagccagc gtcacaaaca
     3181 gcacaggcag ggtcatgata atccctgtac ggaagtaata tccccagctg atagtcatat
     3241 tcttctgcga aagtacgtgc agccagagta gcgtagccag gctaccaatt ggggtaattt
     3301 tcggtcccaa atcgcagcca atcacattgg cataaaccat cgcttctttg ataacgccag
     3361 atgccgtgct gccatcaatg gacaacgcgc caaccagtac cgtcggcata ttgttcataa
     3421 tagaagagag gaaggcggtg aggaatccgg tgccgagcgt cgcggcccac aggccgttat
     3481 ccgccagcac gttgagtacg ccagaaagat attccgttaa tccggcattg cgcaggccat
     3541 aaaccaccag atacatgccg agcgagaaga tgacaatctg ccagggggca ccgcgcagga
     3601 ctttacccgt attaatcgca tgaccgcgtt tagcgacgac aaataatatc agcgcgccca
     3661 cagctgcaat ggcgctcacc ggaatgccga gcggttccag gacgaaaaat cccaccagca
     3721 gaagcagtaa aacaacccag ccagctttga acgtagcagg atctttgatc gcttctgcgg
     3781 gagatttcag cagcgccata tcgtagttct gcggaatatc tttgcgaaaa tagagatgta
     3841 acatcaccag cgtggcaaca atcgcggcga tatccaccgg caccatcacc gaggcgtatt
     3901 cgcgaaagcc gaggccaaag aaatcagcgg aaacgatatt caccaggttg gagacaataa
     3961 gcggcaggct ggcggtatcg gcaatgaatc cggccgccat cacgaacgcc agcgtagtgc
     4021 ctttactgaa ccctaaagcc agcagcatgg cgatgacaat cggtgtcaaa ataagcgccg
     4081 cgccatcatt ggcaaacagg gcggcaacgg cagcaccgag caggacaatc caggtaaaca
     4141 gcaagcgacc acgaccatta ccccagcgtg agacgtgcag cgccgcccat tcaaaaaagc
     4201 cggactcatc cagcagcagg ctgatgataa tgacggcgat aaacgcagcc gtcgcgttcc
     4261 agacgatatt ccacaccacc ggaatatcac ccggatggac cacgcccgta actaacgcca
     4321 gtactgcgcc gagcgttgca ctccagccga tgcctaaacc tttcggctgc cagataacca
     4381 atacgatggt caggacaaag atagcgcctg ccagtaacat aatgcctccc ggataaaaca
     4441 catctgaaaa ttcatatgtg tttagctaaa tttttaactg caaatgttct tactgtcccc
     4501 ggaacagttt tgtcgagcca ggttgcggac aatcgcctga accttttcct gttcacatcg
     4561 ccaggcctca tcaataattt tcgccgccca tgctggaata tgcggtgata agcggtaatg
     4621 aacccactta ccttgcttgc ggtccagcaa tagcccgctt tcacgcagca atgccaggtg
     4681 gcgggagatc ttgggctgcg actggtcgag agcagtgcag agatcgcaga cgcataactc
     4741 tcccagttcg ctgagcagta aaacgatgcc cagacgggtt tcatcagcaa gaattttgaa
     4801 caattggatg ggtaacagaa atgacatatt gcgctcctga ttgttgcagg tagtgtctct
     4861 cttcgaagcg gataagtcaa aaacatatat gacttaacga atgtgtaagt gcagaggaag
     4921 gtaataggtg tgaattttga gttggctatt catttgaaaa gaggtctgaa tcaggacgta
     4981 aaaaaaccga ctttgcgtcg gttttttact ttccagccct gagttggtgg ctctggctgg
     5041 agtgagagag ttcttatcta acagctcaat actattagcc agtggctaac attgcaagaa
     5101 ttagcgattt cttcagctgg cggagggggc acttcggtat atatcgcaat gacgatttat
     5161 atactttcac tgggtcatcg tcatattaag ccttgtccga atccggatgt aaaaaaaccg
     5221 actttgcgtc ggttttttac tttccagccc tgagttggtg gcactggctg gagtgagaat
     5281 gccattatct aacgcatcaa tgctaatacc atactgaaac tattgcaagg acatgctggt
     5341 tttataacct gaatgtactg tatgattatc cagttagctc tgaggcattt tcactctggt
     5401 aatgcgcata aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg
     5461 ctctggccgg agtgagaatg ccattatcta acaatagggc atgcgccgtg acaggcagtg
     5521 gatgagtaag cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc
     5581 aatcatcgtt cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct
     5641 tctgaactga tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa
     5701 catttaacgc attgtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc
     5761 gaagtctttt ttggttgcac ccatcttcag cgccaccgcg aagccctgca acatttcgtc
     5821 cataccaaag ccaatgccgt gaataccgac aatcttctct tccgatccaa cgcacaccag
     5881 cttcatgcgg cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga
     5941 tttatacact ttcacctgat cgtcgccata ctgctcgcgc gcctgcggtt ccgttaaacc
     6001 aacagtacca atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg
     6061 ctcatccggc ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac
     6121 cggtgtcagc tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt
     6181 gttttgatat ttatcgacga cgatatagcc tttttcgtta gttttaacgc cagcggcttc
     6241 caggttgatg ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt
     6301 ttcactgcga ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc
     6361 tttcgggatg gcgttggtgt gcagctgcgg gccttcggcg ttcatcactt cgaccagcgt
     6421 ttcggaaatc atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt
     6481 tttcgcgccg aggccgttaa tcacgcccgc cagctcaacg gcgatgtaac ccgcgccaac
     6541 aaccgccacg cgctctggca aagcaggaag ggcgaagaag ccatcagaat caataccgta
     6601 ttccacgccc ggaatatccg ggtggctcgg acgaccgcct gtggcgatca gaatatgatc
     6661 ggccgtgatg gtttcgccgt ttacctccag cgttttggca tcaacgaagc gggcaaagcc
     6721 tttgattaca tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc
     6781 gatataggcg gtacggctgg cgatcaacgt ttcccagttg aatttattga tagtggtatc
     6841 aaaaccataa tccgggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat
     6901 cacttttttc ggcacacagc caacatttac gcaggtgccg cccagctctt tggcttcaat
     6961 cagcgcacat ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc
     7021 gccgccgatg gcgatgtaat catagtgttt agtcatgaca aagtgtcctt atcgttgatt
     7081 accgcgattg tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg
     7141 atccagctta cggtggcgtg cccggtgcct gccggaacca gtttgctgtg cagccacggc
     7201 agcacgttat tcatctgctg ttccagtttc cacggcgggt taatcacaat catgccggaa
     7261 gcggtcatgc cacggcgatc gctgtctggc agtaccgcca gttcaatttg cagaattttg
     7321 cgaataccgg tcgcttccag atcgtggatc atgcgcttaa tttgctgacg cagcaccacc
     7381 ggataccaca gtgcgtaagt accagtggcg aaacgtttgt aaccttctgc tatcccgctg
     7441 accaccgcct gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg
     7501 cgggaaaccg gcggcagttt ggccttaagc tgctggaaac catcggcttt ttcgacgcgc
     7561 gcacggctgt ctttctgaaa ttcagaacgc agcaacgggt aatcgctcgg gtgcagttcg
     7621 gtcagttgca ggctgtcttg ttcgcgcagt agctggcgag caatcaatgg cgaaccgggg
     7681 tagtaacgca actgaccgct acggttgaag tgttttacca cattgatgta cgcctccagt
     7741 tctgcgggca aatcgtcctg ctgccagata cgggcgatgc cttcgagata ttcgccggta
     7801 cgctcggcat gttcgctgcc taactgataa cgcccggccc ctgcgtgggt gtcgagatag
     7861 agaaacggtt tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta
     7921 aggacgtcgg cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt
     7981 gttccggtaa gtaaaaatcg gcccgtattc ggggcgcaca aaagcgtatc aggacagtat
     8041 accgaaaaga ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta
     8101 accccatgct acacacatta tataaagcgc ctgttgagcg cttccttaac ctctttaacc
     8161 aggactgcgc gaatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa
     8221 attctcccgg aacatgtcgt tccagccgtg actaaggcgc tgaacgactg ccgcgaaaat
     8281 gtggagcgcg tagtagcgca aggagcaccg tacacctggg aaaatctctg ccagccgttg
     8341 gcggaagtgg acgatgtgct ggggcgtatc ttctccccgg tcagccacct gaactcggtg
     8401 aaaaatagcc cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac
     8461 agcacctggg tagggcaaca tgaagggctg tataaagcgt atcgcgacct gcgcgatggc
     8521 gatcattacg ccacgctgaa cacggctcag aaaaaagcgg ttgataacgc actgcgcgac
     8581 ttcgaactct ctggcatcgg tctgccaata gagaaacaac aacgctacgg tgagattgcc
     8641 actcgtcttt ccgagctggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc
     8701 tggaccaaac tcgttaccga cgaagcggag ctggcgggga tgcctgaaag cgcgctggct
     8761 gcggcaaaag cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc
     8821 ccaagctacc tgccggtaat gacctactgc gacaaccagg ccttgcgtga agagatgtat
     8881 cgcgcttaca gcacccgcgc ctccgatcaa ggcccgaacg ccggtaaatg ggataacagc
     8941 aaggtgatgg aagagatcct cgctctgcgt cacgaactgg cgcaactgct gggctttgaa
     9001 aactacgcct ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat
     9061 ttcttaaccg atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggcacaactg
     9121 cgcgcctttg ccaaagccga atttggcgtc gatgagttgc agccgtggga tatcgcgtac
     9181 tacagcgaaa aacaaaaaca gcacctctac agcatcagtg acgaacagct gcgtccgtac
     9241 ttcccggaaa acaaagcggt taacggtttg tttgaagtgg tgaaacgtat ttacggcatc
     9301 accgctaaag agcgtaaaga tgttgatgtc tggcacccgg atgtacgttt cttcgaactg
     9361 tatgacgaga acaacgaact gcgcggtagc ttctacctcg acctgtatgc ccgtgaaaac
     9421 aagcgcggcg gggcgtggat ggatgactgc gtaggccaga tgcgcaaagc tgacggttct
     9481 ctgcaaaaac cggtcgcgta tctgacctgt aactttaacc gcccggtaaa tggtaaaccg
     9541 gcgctgttta cccatgacga agtgatcacc ctgttccacg agttcggtca tggtctgcat
     9601 catatgctga cccgcatcga aaccgccggt gtatccggga tcagtggggt gccgtgggat
     9661 gcggtcgaac tgccgagtca gtttatggaa aactggtgct gggagcctga ggcgctggcg
     9721 tttatctctg gtcactacga aaccggcgaa ccgctgccga aagagttgct ggataaaatg
     9781 ctggcggcga agaactacca ggcggcgctg tttattctgc gccagctgga gttcggcctg
     9841 ttcgatttcc gcctccatgc cgagttccgc ccggatcagg gagcgaaaat cctcgaaact
     9901 ctggcagaaa tcaagaaact ggttgccgta gtaccgtctc catcctgggg ccgtttcccg
     9961 cacgctttca gccatatttt cgccggtggt tatgccgcag gttactacag ctacctgtgg
    10021 gctgacgtac tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt
    10081 gaaaccgggc agtcgttcct cgacaacatt ctgagccgtg gcggttcaga ggagccgatg
    10141 gatctgttca aacgcttccg tggtcgtgag ccgcagctgg atgcgatgct ggagcattac
    10201 gggatcaaag gttagttttt gtgcagattt gcttaattga tgaaacaggc accggagacg
    10261 gtgccttatc tgttctggcg gcccgctggg ggctggagca cgatgaagac aacctgatgg
    10321 cgctggtgtt aacgccggaa catctggaat tgcgcaagcg tgatgagcca aaacttggcg
    10381 gcatctttgt tgattttgtt ggcggagcga tggcgcaccg acgcaaattc ggcggtggtc
    10441 gcggtgaggc ggtggcgaaa gcggtgggca ttaaaggcga ttatttgccg gatgtggtgg
    10501 acgccaccgc agggctgggg cgcgatgcct ttgtgctggc ttcagtcggt tgccgcgtgc
    10561 ggatgctgga gcgtaatcca gtcgttgccg ccttgctcga cgacggcctg gcgcgtggtt
    10621 atgcggatgc ggaaatcggc ggctggttgc aggagcggtt gcagttaatt cacgcctcca
    10681 gcctgacggc gctgactgat attaccccgc gcccgcaggt ggtttatctc gacccgatgt
    10741 tcccgcataa gcagaaaagc gcgctggtga aaaaagagat gcgtgtcttt cagtcgctgg
    10801 tggggccgga tcttgatgcc gatggattgc tggagcctgc gcggctgctg gcgaccaaac
    10861 gcgtggtggt aaagcgcccg gactacgcgc caccgctggc gaatgtcgcc acgccaaacg
    10921 cggtagtcac taaagggcat aggtttgata tttatgccgg aacgccggtg taaaagtaaa
    10981 gcagggtagc gataacgcta ccctgctttc tgcaagaata attaatggct ttccggcgtc
    11041 gcaatcatgc gtttcagcca cggcaccatc aacagcatca ctactgcaac gcccagcgtg
    11101 accagaccaa tcttaccaaa cacgttggta tagacgggca acgtctcaag cggatcggta
    11161 atgttgtccg gcaccgcggt aaatgttgcc acatagccgc ccagcaagaa cgcggcagcc
    11221 tgcgtcagga accacatccc gagaataaag cccatcaaat gctgcggcac cagggcagca
    11281 atcatcgcca gacccagggc gctaataaac aattcaccca ggctctggaa caagtaaacc
    11341 agcacgataa accacggcga tgtcagtcct tgtgcatcag caaaccacat tcccgcagct
    11401 gccgccgtca aaaagcccag tgagcacata aacatgccga gagtaaattt catcggcatc
    11461 gagaggtctt tgcctttgtt acccagatgc gtgtaaatgc ctgccagtat tgggctggcg
    11521 agtaccaccc agaacgggtt aagcgcctgg aagctgaccg ggttgatgga aaagccgaga
    11581 atttcatgat gcacgttgtt gatggcaaag aagttcagcg acgttggcat ctgggcgtag
    11641 agaatgtaaa acaccaccgc ttcgagcatc aggacaaagg cgacaaacat tttattgcgc
    11701 ccggttttat ccagcttgaa tgcctgacga aagaagatga tggtgacgac gatggagaga
    11761 acaatcagca ccagattggc gacttctacg ttgtgcatca gccatgcaca gacgaagatc
    11821 atcaccacgc tgccaagtaa cacgtacaac agtttgctga agctcattgg ccggaagtcg
    11881 ggttcagaac caatgtcttt caccattcca cgacaggcga tgtaaaccag taatgcgata
    11941 attaaccccg ccccgcacag gttgtaggtg actgaataac cgaatctatc agcgatcaca
    12001 ggggccagcg ataacgctat caacgagccg atattgatcg acatatagaa cagggtgaat
    12061 gcgccatcaa gccgcggatc tttcggcgga tagcacttcg aaagcaagct ggctgggtta
    12121 gctttaaaca ggccgttacc gacagcgata gtccccaggg cgatgaaaat caggtcaggc
    12181 ttaagtagcg acatgccggt catgaagtag ccaatcgcca gcacaagtgc tccgagaaca
    12241 atggtgcgtt tggtccccag caggtggtcg ccgacatagc cgccaatgga aatgaggcca
    12301 tagaccagcg cagcaaaagc accaaaagtg acaaaagcct gctcttgcga gaatccaagc
    12361 tgtttaacga agaaaaccgc cagtacgccc tgcacgccgt agtagccgaa tcgctcccat
    12421 aactcgacaa aaaagatcat gaaaaatggg cgaggttgct gcagcatccc catgggtgtt
    12481 gttgtattca tatttattaa ccttccaata ccatcctgaa aagtatgaac gcgtaaggga
    12541 taacgcgcga aaaagtttac atctgtcaga gattcttaat attgatgcgg tgaatagatt
    12601 aatacactat tacaacagaa aataaccagc tgatcacact aatgatggaa aatatgatgg
    12661 tgttaaatgt tgtattggca ggagattttg attgcgtacg cagatcgtag gccagataag
    12721 gggtttacgc tgcatcaggt aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg
    12781 cgttcaggaa cacgtcgtgg ggggatgatt attcttcttc gtcgcgcagc ggaacaatca
    12841 gcatatcaac gtgaacggtg ttgatcagct gacgtgcgga agacatcagt ttgctccaga
    12901 agtcctggtg gtgaccacaa accaccagat ccatatcgta tttcttgatt gcatcgacca
    12961 gaacctggcc caggtcgccg ctgccgctca gggtttcagt gattgggtag cctgcattag
    13021 tggaaagctc ggtcagcgca tgatgtgtct cttcagagat gcgtttctgc atatcaccca
    13081 gattcacatc aataagcccg gtgtataggt cagagtagtt tacatctacg tggatcagag
    13141 aaactttcgc attgtagggg cgagccatag agactgcttt ctctaccaga actttgcttt
    13201 ccggggagag atcgactgcg atgagaatgt gtttataagc catagtgtta ctccttccat
    13261 aaagttgtcg atgactggcc agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc
    13321 gaactacgtg cgcggggcta acccagccgt aaagattcag tgttaagacc atccttacct
    13381 tatagcgacc cggatgatcc gtcaatccgc cttgcttacc cgttagataa acaaattttc
    13441 gacatgaata tattgatagt ggttaacctt ctggaaaaaa aacaacctga tctcctacac
    13501 tatctataga gccgctcgga tgttggtcac cacaaaagcg gtttctccag gacctggtgt
    13561 taactgattg gcagcgtatc gggagcggta gccccggggt ggaactccgc gggcaggtcg
    13621 ccggggagga ggtatgataa gcaccgtcgc attattttgg gctttatgtg tcgtttgcat
    13681 tgttaacatg gcgcgctatt tctcatcact acgcgcgttg ttagtggtac tgcgtaactg
    13741 cgatccattg ctctatcaat atgttgatgg agggggcttt tttacctcac atggccaacc
    13801 caacaaacag gtgcgtctcg tttggtatat ctatgcccaa cgttatcgcg atcatcacga
    13861 tgatgaattt attcgccgct gtgagcgggt gcgtcggcag tttattctga ccagcgcatt
    13921 gtgtggtctg gtggtggtca gcctgattgc attgatgatt tggcattaag cacttcagca
    13981 ataataacgc ataaaaaaag cgggccagta aactgacccg ctcgttttgg tgtgtacgat
    14041 tacaggaact gcaaggagag ccagtacagc ccgccggaaa gcagtaccgc agccggaagg
    14101 gtaaacaccc aggccatcag aatgctggtc acggttttac gctgtaagcc gccaccatct
    14161 accaccatcg tccccgcgac agaagaggag agtacgtgag tagtggaaac cggcatcccg
    14221 gtataactcg ccaggccgat agacactgcc gccgtcatct gggcagacat cccctgagcg
    14281 taggtcatgc ctttcttacc gattttctca ccgatagtcg ttgccacacg gcgccagcca
    14341 atcatcgtac cgatacctaa cgccagcgcg accgccatga tgatccacac cggtgcatac
    14401 tcgatggtgc taagcatgtc agacttcagt ttcttcaaca ggcgctgatc gtcagcactc
    14461 acgccaggca tcttcaccac tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc
    14521 atctggctac gttgatcaag cgacagcttg tcgtagcttt ccacatcggt ggtcaacata
    14581 cctttcaggc ggttgagcgc gttaatggta ttcgacggat ggcagtggaa ctccgcaggt
    14641 tgcgttgcgc cagcttccgg agccggtact aactgatcag caccggtagc ctgtttgagc
    14701 agcgcaggat gctgctcaaa gtaagcttcg acgttgttga tggcatcacg ggtacgggtg
    14761 atttcgtagc cagtggcatt catgttcacc acgaagcctg ctggcgcgac gccaatcaat
    14821 accaacataa ccagaccaat gcctttctga ccatcgttcg cgccgtgcga aaacgccacg
    14881 ccgatagcgg aaaggatcag cgcgatacgc gtccagaacg gcggcttttt cttgccgtct
    14941 ttcttttcac gctccgctgg ggtcaggtgg atacgggcgc gtttcttggt gccgctccag
    15001 tagcgacgca gcaagaaaat cagaccgcca gcaaacacca ggccgacaat aggggaaacg
    15061 atcagagaac cgaaaatact taatactttc gggatattga gtgcatccac cactgacgtc
    15121 ccggtcatca acgcattggt taaaccaatc ccgatgatcg cgccaatcag cgtatgagag
    15181 ctggatgcag gtaaaccaaa gtaccaggta cccaggttcc agataatcgc cgccagcaac
    15241 atagagaaca ccatggcaag gccatgagac gatcccatat taagcagcag atccgtcggc
    15301 agcatatgca caatggcata ggcaacactc agaccaccca gcaaaacacc caaaaagttg
    15361 aacaccgccg ccataaccac ggcgagctga gaacgcatcg cgcgggtata gataacggtt
    15421 gccacggcgt tggctgtgtc atggaaacca ttgatggctt cgtagaacag cacaaaagcc
    15481 agtgcaagca ataataacag cccggtatgc aaatccaggc cagcaaacaa atgtagcata
    15541 ggacgttacg ccattttgag gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg
    15601 ggcaaagtga aatatagact ttttttgatt tgcctcctgt atggat
//
LOCUS       DAZFRN010000118        15565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFRN010000118 DAZFRN010000000
VERSION     DAZFRN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.120"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            121..639
                     /gene="yfiR"
                     /locus_tag="ACHKUB_003566"
     CDS             121..639
                     /gene="yfiR"
                     /locus_tag="ACHKUB_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO6656533.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            629..1855
                     /gene="dgcN"
                     /locus_tag="ACHKUB_003567"
     CDS             629..1855
                     /gene="dgcN"
                     /locus_tag="ACHKUB_003567"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO6656534.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASRHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1871..2353
                     /gene="yfiB"
                     /locus_tag="ACHKUB_003568"
     CDS             1871..2353
                     /gene="yfiB"
                     /locus_tag="ACHKUB_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6656535.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2430..2777)
                     /gene="rplS"
                     /locus_tag="ACHKUB_003569"
     CDS             complement(2430..2777)
                     /gene="rplS"
                     /locus_tag="ACHKUB_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO6656536.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2819..3586)
                     /gene="trmD"
                     /locus_tag="ACHKUB_003570"
     CDS             complement(2819..3586)
                     /gene="trmD"
                     /locus_tag="ACHKUB_003570"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO6656537.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3617..4165)
                     /gene="rimM"
                     /locus_tag="ACHKUB_003571"
     CDS             complement(3617..4165)
                     /gene="rimM"
                     /locus_tag="ACHKUB_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO6656538.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4184..4432)
                     /gene="rpsP"
                     /locus_tag="ACHKUB_003572"
     CDS             complement(4184..4432)
                     /gene="rpsP"
                     /locus_tag="ACHKUB_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO6656539.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4569..5930)
                     /gene="ffh"
                     /locus_tag="ACHKUB_003573"
     CDS             complement(4569..5930)
                     /gene="ffh"
                     /locus_tag="ACHKUB_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO6656540.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6097..6888
                     /gene="ypjD"
                     /locus_tag="ACHKUB_003574"
     CDS             6097..6888
                     /gene="ypjD"
                     /locus_tag="ACHKUB_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO6656541.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6909..8195
                     /gene="yfjD"
                     /locus_tag="ACHKUB_003575"
     CDS             6909..8195
                     /gene="yfjD"
                     /locus_tag="ACHKUB_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO6656542.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVHIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8250..8843)
                     /gene="grpE"
                     /locus_tag="ACHKUB_003576"
     CDS             complement(8250..8843)
                     /gene="grpE"
                     /locus_tag="ACHKUB_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO6656543.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8966..9844
                     /gene="nadK"
                     /locus_tag="ACHKUB_003577"
     CDS             8966..9844
                     /gene="nadK"
                     /locus_tag="ACHKUB_003577"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO6656544.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9930..11591
                     /gene="recN"
                     /locus_tag="ACHKUB_003578"
     CDS             9930..11591
                     /gene="recN"
                     /locus_tag="ACHKUB_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO6656545.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11740..12081
                     /gene="bamE"
                     /locus_tag="ACHKUB_003579"
     CDS             11740..12081
                     /gene="bamE"
                     /locus_tag="ACHKUB_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO6656546.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12143..12433)
                     /gene="yfjF"
                     /locus_tag="ACHKUB_003580"
     CDS             complement(12143..12433)
                     /gene="yfjF"
                     /locus_tag="ACHKUB_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO6656547.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12423..12860)
                     /gene="ratA"
                     /locus_tag="ACHKUB_003581"
     CDS             complement(12423..12860)
                     /gene="ratA"
                     /locus_tag="ACHKUB_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO6656548.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13031..13513
                     /gene="smpB"
                     /locus_tag="ACHKUB_003582"
     CDS             13031..13513
                     /gene="smpB"
                     /locus_tag="ACHKUB_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO6656549.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13728..14090
                     /gene="ssrA"
                     /locus_tag="ACHKUB_003583"
     tmRNA           13728..14090
                     /gene="ssrA"
                     /locus_tag="ACHKUB_003583"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14256..15485
                     /locus_tag="ACHKUB_003584"
     CDS             14256..15485
                     /locus_tag="ACHKUB_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO6656550.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
BASE COUNT         4004 a         4051 c         3660 g         3850 t
ORIGIN      
        1 gattttactc attgtgtttt atttctcaca ttgatgacga tcgcatgaaa aaatgatatt
       61 ttaatgagca ttctgaaaaa atatcgatgc atttcgagcg aagatggtgg ggatccctga
      121 atgcgttttt ctcaccgact gttcttactt ctgattctcc tgctgacggg agcaccaata
      181 ctcgctcagg agccttctga cgttgcgaaa aacgtgcgta tgatggtttc cggtattgtc
      241 agttacaccc gctggcctgc gctatcaggg ccaccaaagt tatgtatctt ttcatcttca
      301 cgttttagta ccgccctaca agagaatgcc gcgacatctt tgccttatct gcctgtcatc
      361 attcacactc aacaagaggc gatgatttca ggctgcaatg gtttttattt tggcaacgag
      421 tcaccgacat tccagatgga attaaccgaa caatatccgt caaaggcgtt attattaatt
      481 gccgaacaaa ataccgagtg cattattggt agcgcctttt gtctgatcat ccacaataat
      541 gacgtcagat ttgccgtaaa cctggatgcc ttatcgcgta gcggtgtaaa ggtcaacccg
      601 gatgtcttaa tgctcgcacg gaagaaaaat gatggataac gataattctc ttaataagcg
      661 ccccacgttt aaaagagcat tacgcaacat cagtatgacc agcatattta tcactatgat
      721 gctgatctgg ttgctgcttt ccgtgacctc ggtgctgacc ctgaaacagt acgcgcaaaa
      781 aaacctggca ctgacagcag caacaatgac ttacagtctg gaagcagctg tcgtttttgc
      841 cgatggccct gcagcaactg aaacactggc agcgctgggc cagcaagggc aattttcaac
      901 tgcagaagta cgtgataagc agcaaaatat tctggcgtcc aggcattaca cccgtaagga
      961 tccaggcgat actttcagca atttcataag ccactggctc ttccccgccc ccatcattca
     1021 gccgattcgt cacaatggtg aaaccattgg cgaagtacgc ttaaccgctc gcgacagttc
     1081 aatcagccat tttatctggt tttcgctcgc cgtactgacc ggttgtattc tgctggcatc
     1141 aggcatcgca attaccctca cccgccattt gcacaatggc ctggtagaag cgctgaaaaa
     1201 tatcaccgat gtcgtacatg atgtgcgttc caaccgcaat ttttcccgac gagtttcgga
     1261 agaacgtatc gctgagtttc accgcttcgc tctcgacttc aacagtctgc tggatgaaat
     1321 ggaagagtgg cagcttcgtt tacaggccaa aaatgcgcag cttctgcgta ccgcgctaca
     1381 tgacccatta accgggctgg ctaaccgcgc agcgtttcgt agcggcatca atacgttgat
     1441 gaacaattcc gatgcccgaa aaacgtcggc gttactattt cttgatggcg ataatttcaa
     1501 atatatcaat gatacctggg gtcatgcgac gggcgataga gtcttgattg aaatcgcaaa
     1561 acggttagct gaatttggcg ggctgcgaca taaagcatac cgcctgggcg gcgatgaatt
     1621 cgctatggtg ctctatgatg tacagtcgga atctgaagtg cagcagatat gctcagcact
     1681 gacacaaatc tttaatctcc cgtttgatct tcataatggc catcagacca ccatgacatt
     1741 aagcattggt tacgcgatga ccattgagca cgcctctgcg gaaaaattac aagagcttgc
     1801 cgatcacaat atgtatcagg ccaaacacca gcgtgccgaa aagctggtga gataacaagg
     1861 atatatcgat atgataaagc acctggtagc acccctgatt ttcacctcac taatactgac
     1921 tggctgccag tcccctcagg gaaagtttac tcctgagcaa gtcgccgcta tgcaatctta
     1981 tggatttact gaatccgccg gcgactggtc gctgggctta tcagatgcca ttctgttcgc
     2041 aaaaaatgac tacaaattgc tcccggaaag ccagcaacag atccaaacca tggcagccaa
     2101 attggcctcg acagggctaa cacatgcccg tatggatgga cacaccgata actatggtga
     2161 agacagttac aacgaaggct tatcattgaa acgggcgaat gtcgtggccg atgcatgggc
     2221 tatgggtgga caaattccac gcagcaatct caccacacag ggtttaggaa aaaaatatcc
     2281 catagccagt aacaagaccg cccagggccg cgccgagaac cgccgcgtcg cagtggtgat
     2341 tacaacccct taaaacacga gcaaacagac aaaaaaaggg ccagccaatt ggccagccct
     2401 tcttaacagg atgtcgctta agcgaaatct tagttaagac gctctttgat acgagcagcc
     2461 ttaccagtac gctcacgcag gtagtacagt ttagctttac gaacagcacc acgacgtttg
     2521 acagaaatgc tgtcaactac cggagagtga gtctggaaga cacgctcaac gccttcgccg
     2581 ttggaaattt tacgaacagt gaatgcagag tgcagaccgc ggttacgaat agcgataacc
     2641 acgccctcga atgcctgcag acgttttttg gaaccttcaa caacccatac tttcacttcc
     2701 acggtatcac ccggacggaa ggaaggtacg tcctgcttca tctgctcttg ttcaagttgc
     2761 ttaataatgt tgctcataat ttaatctctt atcctgggta aactgatatc tcgggggctt
     2821 acgccatccc atcatgttta tgttgctgtt gtgcgtgttc cgttttgaac tccgccagca
     2881 accttgcttg ctcttcagtc agagccaggt tttccagaag ttcaggtctt ctaagccagg
     2941 tacggcccag cgactgtttc aaacgccagc gacgtatctc ggcatggttg cccgacagta
     3001 acactggcgg aacttccatc ccttctaaca cctcaggccg cgtatagtgc gggcaatcca
     3061 gcaatccttc agcaaaggaa tcttccgttg ccgaggcttc atgtcccagt actcccggaa
     3121 taaaccggga aacggagtca atcagcgtca ttgctggtaa ctcaccacca ctgagaacgt
     3181 aatcgccgat tgaccattct tcgtcaattt cggtttggat cacgcgctca tctatacctt
     3241 cgtagcgacc gcacaccaga atcaattttt gattcgttgc cagttcgctg acgcccgctt
     3301 gatcaagctt gcgtccctgt ggtgacagat aaatcacctt tgcgccttca cccgccgcgg
     3361 cttttgctgc atgaatggcg tcccgcaagg gttgcaccat cattaacatc cccggtccgc
     3421 cgccgtaagg acgatcgtcc acggtacggt gccggtcatg cgtgaagtcg cgaggactcc
     3481 aactctggat gctcagcagg ccatttttaa ctgcccggcc agttaccccg taatcggtaa
     3541 ttgcgcggaa catttcagga aacaggctaa ttatgccaat ccacatagcg ccgtctttta
     3601 ccgtttatcc ggtggtttaa aaaccaggat cccaatctac ttcgattgaa cgagtagtga
     3661 gatcgacttt cttgataacc tgcccatcga ggaacggtac gagacgttcc ttgataccaa
     3721 acgcatcttt caggtttgcc ttaatgacga gaacgtcatt agatccggtt tccatcatat
     3781 cgacgacttt accgagatcg tagccttcag tggttactac ctggcagccc atcaggtctt
     3841 tccagtagta gtcaccctct tcaagctgag gcagctgcga tgaatccacg acaatttcac
     3901 aattcgtcag caggttcgcc gcatcacgat cgtcaacgcc tttcagcttg atgatcatgt
     3961 cctgattgtg gtgcttccag ctttccagct ggacttgctg ccactgaccc gccttctgga
     4021 taaaccaggg ctgatagtca aaaatgcttt cggcgtcttc ggtggaagaa aacactctga
     4081 gccacccacg aataccgtaa gacgaaccca tttttcccaa aacgatggga tcaacaggtg
     4141 cttgcgcggt gagttgtttg ctcatcatga ccaccgtgac agattaagct gctttgttta
     4201 cttctttgat cagcgcagca acgcgatcag aaatagttgc gccctggcca acccagtgag
     4261 cgatgcgatc cagatccagg cgagtgcctt cttctttttc gctagcgatt gggttgaaga
     4321 aaccaacgcg ctcgatgaag cgaccgttgc gtgcattacg gctgtcagcg acaacaacct
     4381 ggtagaacgg acgcttttta gcgccgtgac gtgctaaacg aatagttacc ataacatcct
     4441 cttgtgtgaa taaaacaacc ggaccccatc gaggaacgga gtccggtgtc atattaaaag
     4501 cccgaaaatt ttactgattt ctggagaaaa tgcaatcagc agttaataac tctgtcgcat
     4561 aaggtcattt agcgaccagg aaatcccggc ggcatcatcc ccttcatgct tctcatcatc
     4621 ttcgccattc cgcccttctt cattttcttc atcatgcgct gcatgtcgtc gaactgtttc
     4681 agaagacggt taacgtcctg cacctgcata ccgcaaccgg cagcaatacg gcgtttacgc
     4741 gaacctttga tgatttctgg cttagcgcgc tctttcatcg tcatcgagtt gatgatggct
     4801 tccatacgca ccagcacttt atcgtccatc tgcgacttaa cattatccgg gatctgcccc
     4861 atgcccggca gcttgcccat caggctagcc atgccgccca tatttttcat ctggcgcagt
     4921 tgctcaagaa aatcgttgag atcgaagccg tcgccttttt tcagcttgct ggctaatttc
     4981 tctgcctgcg cgcggtcaac tttgctttcg atatcttcga tcagcgacag tacgtcgccc
     5041 atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc ctcagttttc
     5101 tcgccaacac cgagaaactt gatcggtttg ccagtgatgt gacgaataga gagcgccgca
     5161 ccgccgcggg catcgccgtc cactttggtc aacactacgc cggtaagcgg taacgcttca
     5221 ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac cacaaacagg
     5281 gtttcaaccg ggttaatcga cgcatggact tgtttgatct cgtccatcat cgcttcgtca
     5341 acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag tttggcttct
     5401 ttcagcgccg cgttaacgat atctaccggc ttctgaccaa catcagaagg gaagaaatca
     5461 acgcccacct gctctgccag cgtctcaagc tgtttgattg ccgccgggcg ataaacgtcg
     5521 gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca ggaacttacc gagtttacca
     5581 acgctggttg ttttaccggc accttgcagg cccgccatca gcacgaccgc aggcggttgc
     5641 gccgccaggt tcagggtctg gttctcttcg cccatcgccg caaccagttc gttacggacg
     5701 attttgacga actcctgccc cggcgtcagg ctcttattaa cttcatgacc aaccgctttc
     5761 tcttttacgc gattgataaa ctcacgcact accggcagag cgacatccgc ctccagcagc
     5821 gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg tccacggcca
     5881 ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat tgtctctcgc
     5941 ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc cgcagtataa
     6001 catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga cgctatactg
     6061 cttctctttc ttattgctca aactgtcgac atcactatgc ccgtttttgc tctgctcgcg
     6121 cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca aaaaaacggc
     6181 ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca cgcaatcgct
     6241 ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct gctgaacgtt
     6301 ggttcattgg tcagtttgat gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt
     6361 ggctggttgc tgctacccat tgtctatgcc tttgcgctta tcaacctggc gctggcgacc
     6421 ttcatgccca atgaatacat cacccatctg gaagcaacgc ctgggatgct ggtgcacatt
     6481 ggcttatcgc tcttttccta tgccacgcta attatcgccg ccctgtacgc gctgcaactg
     6541 gcgtggattg attaccaact gaagaacaag aagctggcat ttaaccagga aatgccgcca
     6601 ttgatgagta tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt gctgctaacg
     6661 ctcacgcttt gcactggcct gttctacatg cacaacctat ttagcatgga aaatatcgac
     6721 aaggctgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctgtg gggacattac
     6781 catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg ttgcgggcgc ggtaattctg
     6841 acactggcct acttcggcag ccgaattgtc cagcagttaa tcagctaaac ccagaaaagg
     6901 agtttcccct ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg
     6961 tcatttcagc ctatttttcc gggtccgaaa ccggaatgat gaccctcaac cgctatcgtc
     7021 tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta
     7081 agccagaccg cctgataagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct
     7141 ccgcgctcgg cactattgtt ggaatgcgtt tgtacggcga tgcgggcgtg gcaattgcga
     7201 ctggtgtgct gacttttgtc gtactggtat ttgctgaggt attgccgaaa accattgccg
     7261 cgctgtaccc ggaaaaagtc gcttatccga gtagttttct gctggctccg ctgcaaattt
     7321 tgatgatgcc gctggtctgg ttgctgaacg ctatcacccg catgttaatg cgcatgatgg
     7381 gcatcaaaac cgatatcgtg gtcagcggct ctttgagcaa agaagagttg cgcactatcg
     7441 tgcatgaatc gcgctcacaa atttcccgtc gcaatcagga tatgctgctg tcggtgctcg
     7501 atctggaaaa aatgaccgtt gatgacatca tggtgccgcg cagtgaaatt atcggtattg
     7561 atatcaacga tgactggaaa tcgattctgc gccaactctc ccactcacct cacgggcgca
     7621 tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat gctgcgtgtg cgtgaagcct
     7681 ggcggttgat gtcggagaaa aaagagttca ccaaagaaac catgctacgc gccgcggacg
     7741 agatctattt tgtcccggaa ggtacgccgc tcagcacgca gttggtgaaa tttcagcgca
     7801 acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga cattcagggg ctggtgacgg
     7861 ttgaagatat tctggaagag attgtcggcg atttcactac gtcgatgtcg ccaacacttg
     7921 ccgaagaggt cacgccgcaa aacgacggtt cggtgattat tgatggcact gccaacgtgc
     7981 gggaaatcaa caaagccttt aactggcatc tacctgaaga cgatgcccgc acggttaatg
     8041 gcgtcattct tgaagctctg gaagagatcc ctgtcgctgg cacccgcgtg catattggcg
     8101 agtacgatat cgatattctc gacgttcagg acaatatgat taagcaggta aaagtctttc
     8161 ctgtaaaacc gctgcgcgag agtgtggcgg agtaacgaaa acggccctgc attcgcatgc
     8221 agggccgtga attattacga aaacagaaat tacgcttttg ctttcgctac agttaccatc
     8281 gccgcacgaa tcgtacgacc attcagcgta taacccttct gcataatgcc cagtacgtta
     8341 cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct gatgcacatt cgggtccagc
     8401 ggcacgttag tttcggcgat cacttcaaca ccaaacttac gcacaacatc cagcatcgac
     8461 ttcagcgtca gctcaatgcc ttcaaccatc gcagacatat ccgggttagc tttatcagcc
     8521 acttccagcg cacgatccag gctatcaatc accggcagca attcgttgat gaatttctcc
     8581 agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac gtcgacgcag gttttccatt
     8641 tcggctttta cacgcaaaat gccgtcacgt tcacgggtct gggcttcagc cagctgagct
     8701 tcgagattcg caattttttc atcgcgcgga tccacctgct cagcagaagc ttctggctca
     8761 actgcctcaa tctcttcgtg ctgatccatg ataatttctt ccggggcttg cccctcaggc
     8821 gttttctgtt ctttactact catgaatttc tccgcgtttt tttcgcattc atctcgctaa
     8881 cttcgcttat tatggggatc agtttcaggg tttcaaggga agcactcaca ttgtcatcaa
     8941 tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc aagtgtattg gcattgtggg
     9001 acacccacgg caccctactg cactgacaac acatgaaatg ctctaccgct ggctgtgcac
     9061 aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac gaactgcaac tgaagaatgt
     9121 gaaaactggc acgctcgcgg agattgggca actagctgat ctcgcggtag tcgttggtgg
     9181 cgacggtaat atgctgggcg cggcgcgcac actcgcccgt tacgatatta aagttattgg
     9241 aatcaaccgt ggcaacctgg gtttcttgac tgaccttgac cccgataacg cccagcaaca
     9301 gttagccgat gtgctggaag gccactacat cagcgagaaa cgttttttgc tggaagcgca
     9361 agtctgtcag caagattgcc agaaacgcat cagcaccgcg ataaatgaag tggtgcttca
     9421 tccaggcaaa gtggcgcata tgattgagtt cgaagtgtat atcgacgaga tctttgcgtt
     9481 ttctcagcga tctgatggac taattatttc gacgccaaca ggctccaccg cctattccct
     9541 ctctgcaggc ggtcctattc tgaccccctc tctggatgcg attaccctgg tgcccatgtt
     9601 cccgcatacg ttgtcagcac gaccactggt cataaacagc agcagcacga tccgtctgcg
     9661 tttttcgcat cgccgtaacg acctggaaat cagttgcgac agccagatag cactgccgat
     9721 tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac catctgaatc tgattcatcc
     9781 gaaagattac agttatttca acacattaag caccaagctc ggctggtcaa aaaaattatt
     9841 ctaattttac gccagcctct ttactgtata taaaaccagt ttatactgta cacaataaca
     9901 gtaatggttt ttcatacagg aaaacgacta tgttggcaca actgaccatc agcaactttg
     9961 ctatcgttcg tgagcttgag attgattttc atagcggcat gaccgtaata actggcgaga
    10021 ccggcgcggg taaatctatt gcaatagatg ccctcggtct ttgtctcggt ggtcgcgctg
    10081 aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct gtgcgcccgt ttttctctga
    10141 aagatacgcc agcggctctg cgctggctgg aagaaaatca gcttgaagac gggcatgaat
    10201 gtttgcttcg tcgagtgatc agcagcgatg gtcgctcccg tggtttcatc aacggtacag
    10261 ctgttcctct gtcacaactg cgcgaactgg gtcagttgct gattcagatc catggtcagc
    10321 acgctcatca attactcacc aaacctgagc accaaaaatt cctgcttgat ggctatgcca
    10381 atgaaacctc tctactgcag gaaatgaccg cacgttatca gttgtggcat caaagctgcc
    10441 gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc cgcccgtgcg gaactgctgc
    10501 aataccaatt aaaagaactt aacgaattta atccgcagcc cggagagttt gaacaaatcg
    10561 acgaagagta caaacgtctg gcgaacagcg gtcaattgct gaccaccagc cagaatgcat
    10621 tggcattaat ggccgacggt gaagacgcaa acctgcaaag tcagctttac acggctaaac
    10681 aactggtgag cgaattgatt ggcatggaca gcaaactgtc cggcgtactt gatatgctgg
    10741 aagaagctac catccagatt gctgaagcca gcgatgaact gcgccactac tgcgatcgtc
    10801 tggatctcga tcccaaccga ctatttgaac ttgaacagcg catctcaaaa cagatttcgc
    10861 tggcacgtaa acatcacgtc agccctgagg cattgccaca gtattaccag tcgctactgg
    10921 aagaacagca gcaactggac gatcaggccg actcacaaga aacgcttgcg ctggcggtaa
    10981 cgaaacatca tcagcaggca ctggaaatcg cgcgcgcatt acaccaacaa cgccagcaat
    11041 atgcagaaga acttgcacag ctgatcaccg acagtatgca tgcgctctca atgccgcatg
    11101 ggcagtttac gatcgatgtt aaatttgacg agcatcacct gggcgctgac ggtgccgatc
    11161 gtattgagtt tcgggtaacc accaacccag gtcagccaat gcagcctatt gccaaagtcg
    11221 catccggtgg tgaattgtcc cgcatcgcac tggcaatcca ggtcatcacg gcgcgtaaaa
    11281 tggaaacccc ggcactgatt tttgatgaag tggatgtagg tattagcggt ccaacagcgg
    11341 cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac tcaggtgatg tgtgttaccc
    11401 acctgccaca agtcgcggga tgtggtcatc aacactattt tgtcagcaaa gaaaccgatg
    11461 gtgcgatgac agaaacgcat atgcaatccc tgaataaaaa agcgcggtta caagagctgg
    11521 cgcgcctgct tggtggcagt gaagtcacac gtaatacact ggcgaatgcg aaagaactgc
    11581 ttgcagcgta aacttttttc ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac
    11641 cggaaagcaa aaggttttaa agtgatgaaa ggtctattat catcggcata ttacagatga
    11701 gccacgtact gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa aacgctgact
    11761 gctgcagcag cagtactatt gatgttgacc gcaggctgtt ccactctgga gcgagtggtt
    11821 taccgtcctg acatcaacca ggggaactat ctgaccgcta acgacgtatc caaaatacgt
    11881 gttggcatga cgcaacaaca agttgcgtac gcattgggta caccgctgat gtccgatcca
    11941 tttggtacga atacctggtt ctatgtcttc cgccagcaac caggtcatga aggtgtaacg
    12001 cagcaaacgc tgacgctgac ctttaacagt agcggtgtgt tgaccaatat tgataacaaa
    12061 cctgcgctga gtggtaacta ataataaagt tgctctcaaa gacgttaaaa aaggtgctca
    12121 atgagcacct tttttctgtc tgttatttat tcgctgattt ttctgctcgt tgcctgcgaa
    12181 gctctttcgg atcggcaatg agagggcgat aaatctccac ccgatcgcca tcatgcacac
    12241 tatcgcttag ttttgccgga cggctgtaaa tgccgacttt gtttttagtt aaatcgatat
    12301 cggtacgcaa ttccagcaag ccactggcgc gaatagcttc ttcaaccgtc gcgccctcct
    12361 gcagcgtcac gcgctgcagg tactgcttct caggtagcgc ataagccacc tcaacggcaa
    12421 ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa aagcctggac catattagac
    12481 gccagctctt taaacacgcg accaaaggcg agttcaatca acttattggt aaactcaaag
    12541 tcgagatgaa actcgatacg acacgcctcc tggctcagcg gcgtaaactt ccatccacca
    12601 atcaatttct tgaacggccc atccaccaga ttcataagaa tactttggtt actggtcaac
    12661 tggttgcggg tagtaaacgt tttgctgatc ccagccttag agacatctac cgccgcagtc
    12721 atctgcccag gagtggactc cagaatccga cttccggtac aacccggcaa aaactgagga
    12781 taagactgaa cgtcattcac taactgatac atttgctccg cgctgtaggg taccagtgcg
    12841 gtccggctaa tctgaggcat aacaatttcc atcaacaaaa atccaacaaa taatatcatt
    12901 tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa catcgggttc atgctaagat
    12961 agagccttgt cccccgcagg attgatatgg ggtgttttcg atttcagatt accgatgatt
    13021 cacgacgctt atgacgaaga aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa
    13081 caagcgcgcc cgtcacgaat actttatcga agaagagttc gaagcgggac ttgccctgca
    13141 aggctgggag gttaaatccc tgcgcgcagg aaaagccaat atcagcgaca gctacgtcct
    13201 tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc acgccaatgg ccgtggcctc
    13261 cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc agcgcgaact
    13321 ggactcattg tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc tctccctgta
    13381 ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaaa aacagcacga
    13441 taaacgttca gatatcaaag aacgcgaatg gcaggtggat aaagcacgta tcatgaaaaa
    13501 cgcccaccgt taaacctgca ctccaattat tgaccagttc ctcaccgcgc ctccctctcc
    13561 ggcggcgcga atgaacatct tattggctat cacatccgac acaaatgttg ccatcccatt
    13621 gcttaatcga ataaaaatca ggctacatgg gtgctaaatc tttaacgata acgccattga
    13681 ggctggtcat ggcgctcata aatctggtat acttaccttt acacattggg gctgattctg
    13741 gattcgacgg gatttgcgaa acccaaggtg catgccgagg ggcggttggc ctcgtaaaaa
    13801 gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat aacctgctta
    13861 gagccctctc tccctagcct ccgctcttag gacggggatc aagagaggtc aaacccaaaa
    13921 gagatcgcgt ggaagccctg cctggggttg aagcgttaaa acttaatcag gctagtttgt
    13981 tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag actgactaag catgtagtac
    14041 cgaggatgta ggaatttcgg acgcgggttc aactcccgcc agctccacca ctttttagtt
    14101 gtttgaagtt caatgaagtc tactaagccc acacagcaca agctctgcgg gcttttttac
    14161 gtctattgtc gtccagtgag aattgctgag aactacgagt tatggcaccc tgaatgggac
    14221 ccactaagaa gggtccaaaa accgagggtc ccaaaatggc aaaaatcgct aagaagctca
    14281 ctgacactga aatcaaaagc accaagccag ccgataaaga aatcaacttg tttgacggtg
    14341 atggtctgat tctacgaatc gctcctttgg cgaaaggagg caagaaaaat tggtatttca
    14401 ggtatgcagt accagtgagc aagaaaagaa ccaaaatgag ccttgggaca tatcctcacc
    14461 ttacccttgc aagagccaga gccttacgtg atgaatatct ctcctttctg gcaaatggtg
    14521 ttgatcccca aatccataac aacgataagg cgaaggcatt aaagagtgct actgagcaca
    14581 ctctccaagc cgtagcgcgg aaatggttag atgagaaggt aaagacatca ggtatctcac
    14641 aagaccatgc agcagacatc tggcgcagct tagagagaaa tgtctttccc ggtctgggta
    14701 atgtccctat caatgagatc cgacctaagc tcttaaaaca acaccttgat cctattgagc
    14761 aacgaggcgt attggaaact ctacgccgta tcatttcacg tctgaatgaa atcttccggt
    14821 gggcagctac tgaagaactt attgagttca acccggctga caaccttggt caaagattca
    14881 gtaaaccaaa aaagcaaaat atgcctgccc ttcccccaag cgaattgcca aggtttatgg
    14941 aatctttgac gaatgcgtca atccggttgg aaacacgtat gctaattgaa tggcaattgt
    15001 tgacatgggt tcgtccgggt gaagccgttc gcgcaaggtg gtctgatatt gatacaacca
    15061 acagcatttg gaacattcct gctgatttca tgaaaatgaa aaagcttcac aaagttcctt
    15121 tgagtaaaga agctttgcgc atccttgaat taatgaaatc aataagtggg catagagaat
    15181 gggttttccc cagcataaaa gcgcctctta atcatatgca tgaacaaaca gccaacgcag
    15241 ctatcatccg aatggggttc ggaggcgagc ttgtagctca cggtatgcgt tctattgcac
    15301 gaacagcggc agaggagtct ggtaaattca gagctgaagt tcttgaggca gcgcttgccc
    15361 actcgaaaaa agatgaaatt atcgcagcat acaatcgtgc agaatatctg atagagcgac
    15421 agagtttgat gcaatggtgg agtgattacg ttcaagctca aagatcaaat gctctggtag
    15481 cctaagtatc agaatagcta atataatcct gaaggtaaag aaaatggaaa ccctattcaa
    15541 agtttttgaa aaatttagtt ccaga
//
LOCUS       DAZFRN010000119        15460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFRN010000119 DAZFRN010000000
VERSION     DAZFRN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.121"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(542..1003)
                     /locus_tag="ACHKUB_003585"
                     /pseudo
     CDS             complement(542..1003)
                     /locus_tag="ACHKUB_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1311..1424
                     /locus_tag="ACHKUB_003586"
     CDS             1311..1424
                     /locus_tag="ACHKUB_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO6656551.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
     gene            complement(1669..2373)
                     /locus_tag="ACHKUB_003587"
     CDS             complement(1669..2373)
                     /locus_tag="ACHKUB_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HFO6656552.1"
                     /translation="MRRLPLMVGVIVGVMLAVGCHDRRAGVKTLLGDGMDQWGQKLPY
                     DHWQFNFFYPKNLPALVTMVYLEDGDVRESIFRQLDAARPSQSSVDTWSQRVGSFSAS
                     FNIGKALPVRMTVCWDSVIDKKAYETEIWFSRDTWQQMLTAYPDTYKPGKTYYRDNMI
                     IGLAPGGAVRIWLEDNGEPVVLQHPARQFTLTGDDMLICKNVPNQIDFSYIKANGYDP
                     FIRDFIKEKPYPYGHW"
     gene            complement(2384..4075)
                     /locus_tag="ACHKUB_003588"
     CDS             complement(2384..4075)
                     /locus_tag="ACHKUB_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFO6656553.1"
                     /translation="MVEEDEKNVPGDTCYYRYEIIGDSTHRTSIHNSFKTIQCRRVPV
                     RGITLTVGVFFDGTGNNRANADDLRLAYAHCANLVDEERARACEKYEKRARAGLGNAS
                     WQGGHTNISRLFDLYQQSDELNDSETEAQVKAYISGIGTADGESDSILGKALGSSLIR
                     QFEGVVTKTDEALEKISAELTRFIKLNRDKVAIAKVQFDVFGFSRGAAAARHFANRVM
                     NQDAAIAAAIDKGTDLTGHHGKLAGEVRFLGLFDTVAAIGSLLNFYDVNGRSNPGVNL
                     ELRPSVAQKVFQISAMHECRYNFSLNSIAGMWPELALPGAHSDIGGGYNAGEDEISLL
                     TMPDFEVTRDSADEMQTEVYRKAEKMRQVLYSLPALKYLMPHGEVETKMISWPLVNQN
                     KARASIFEKKVGAAVFMTRRDVPNDWEKVSLRVMLDAAQDAGAVFTPILSSNKELALP
                     SELLPLCDKAIAQGRAVRRGAEPEGFTAEDMRTIGRYLHCSANWNIDSDLSLWVSPSS
                     GEVFLRNNYAPTDERSFVWPMAPGKGWVRTVWRMDDQQQWEERARVNADALDGLF"
     gene            complement(6263..6457)
                     /locus_tag="ACHKUB_003589"
     CDS             complement(6263..6457)
                     /locus_tag="ACHKUB_003589"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO6656554.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            complement(6715..7182)
                     /locus_tag="ACHKUB_003590"
     CDS             complement(6715..7182)
                     /locus_tag="ACHKUB_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656555.1"
                     /translation="MRDKTREAMRFFLGGRCYTAENLEKDYLAEVANYSDDRWEAPQR
                     AARLAAAVKRYKTSEMLRFIFATTAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRIYQARIKAS"
     gene            7342..7596
                     /locus_tag="ACHKUB_003591"
     CDS             7342..7596
                     /locus_tag="ACHKUB_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFO6656556.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            7596..8012
                     /locus_tag="ACHKUB_003592"
     CDS             7596..8012
                     /locus_tag="ACHKUB_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO6656557.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCSRLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            8910..9272
                     /locus_tag="ACHKUB_003593"
                     /pseudo
     CDS             8910..9272
                     /locus_tag="ACHKUB_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            9276..9631
                     /locus_tag="ACHKUB_003594"
                     /pseudo
     CDS             9276..9631
                     /locus_tag="ACHKUB_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            9912..11051
                     /locus_tag="ACHKUB_003595"
     CDS             9912..11051
                     /locus_tag="ACHKUB_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFO6656558.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            11171..12439
                     /locus_tag="ACHKUB_003596"
     CDS             11171..12439
                     /locus_tag="ACHKUB_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO6656559.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRTLLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            12447..13592
                     /locus_tag="ACHKUB_003597"
     CDS             12447..13592
                     /locus_tag="ACHKUB_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFO6656560.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            14060..14206
                     /locus_tag="ACHKUB_003598"
     CDS             14060..14206
                     /locus_tag="ACHKUB_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656561.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
     gene            complement(14277..>15460)
                     /locus_tag="ACHKUB_003599"
     CDS             complement(14277..>15460)
                     /locus_tag="ACHKUB_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6656562.1"
                     /translation="QTRVFNEHINELVITRRNGKVITVNDGREMIEFVSCSYLGLETH
                     ENLKHAIANAIERFGVQLSVARTRVKVDLFEQLEKRLNKIMNGAHTLTFNAVTPCHLA
                     VLPLLASGTLPNYKFNKKPYFILEKTAHATLQINRGLLQQFGETIRVDFQHKNDIEEA
                     FKYASINNMVPISISDSVGSMGGVAPVEFIVRMANRFDGYAYIDDAHGMSIYGKNGSG
                     YVMSCLDNKLDDRIIIAGSLSKAFGSHGGFIACNYPDTINFIKTYGTTYAFGGPPSLP
                     GIAACIAAADLHLDGTVSLRQNDLQQVIKYFDDKFNETEVINKGTTMPIRGLLIGNEE
                     KAITICKKMHDRGFATTVAMYPTVEEGKAILRCAISAIHTRENIDSLHKNFIECINEQ
                     G"
BASE COUNT         3844 a         3956 c         3604 g         4056 t
ORIGIN      
        1 tttcacttcg cagcaaaaaa cgaaattcta caatttttct gattttactg acagaaaagc
       61 ctgagagtta aatttaaata acttaaaaga ttatgtggta taatcaggtt catacaggcc
      121 tgattatttt acggggatgt taactctttc aaatcaacag tctacggaga agcaatgtgg
      181 ttatggtcaa taaatgttca ctgatttctt tcacggtaat acccggggca cttcttgtcc
      241 tctggtaaca aaggtaactg tacattcgtc tttgcaactg acagtgcatc acggttttga
      301 agtacctgct gctgttgttt ttttactcag aaagcgcagt tactctttat attttcccag
      361 cctatctctt attttaatat acatccactg tctggcgttt taaggggctg gctcactggc
      421 caagttgaca ttaatcatca ttcctgtctt tgtcagcgga gacttctgct gtcagttttt
      481 tcatccatac agagtgacat taattatccg taagcgcacc gtgaaggacg tggggtaaaa
      541 attagtttac agatagagtg acgttccagg gcaacaactc tttcacgcgg ttggctggcc
      601 aggtgttgat gacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt
      661 tgcagctacc gatcaggctg tacatcactg ccgcactctc gcctcctcca tcagagccga
      721 agaacatgta gttacgccgc cccagtgcaa ctacccggag ggcgttttca cacagattat
      781 tgtcgatctc cacccagcca ttgcggcagt attcgttcag ggcgtcccat tgcttcagca
      841 gataggtgaa cgctttcgcc gtatccgagt gacgcgacag cgtgctcatc tgcccctgga
      901 accactcata caacgactgc attagcggta ccgttctggc ttttctgacc gccagtcgct
      961 cttctgccgg actgccgcgg atctcagact cgatagcgta cagtgcctcg atagggctgc
     1021 gtttgggaaa cgtacaatat tgtacggttg cgctattgcg gtagggtgta ctccaacgct
     1081 atacggtatt tggcagacct ttagggacat gagataacct aaatgcaata agagaaaagc
     1141 aataacttaa aggcgatagc tgatcacgat gaatcaatca gttacttatc cgcatcgatt
     1201 aaaaaatgct cctaacgtac aataattccg tttcccaaac cgacccctct catagctcac
     1261 gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt atgcacgaac
     1321 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1381 taagacacga cttaacgcca ctggcagcag ccattggtaa ctaaacaagg tggattccga
     1441 tagttagagt cttgaagtga tcacccaact acggctacac tgaaaggaca gtattcggta
     1501 tctgcgctcc accaagccag ttaccacagt cgattagaag aggtcgaaga cttctaatcg
     1561 gacggaaaaa gccgccgcaa ggcggctttt tccgtttaca agccgaagat tacgcgcagc
     1621 ttaaaaggat ctcaagaaga tcattttaat tttgctgatc gttgctccct accagtgacc
     1681 atacggatag ggcttttcct tgataaaatc gcggataaat ggatcgtaac cattagcctt
     1741 gatgtaactg aaatcaatct ggttgggaac gtttttgcat atcagcatat catctcccgt
     1801 cagagtgaac tgccgggcag gatgttggag tacaacaggc tcgccattat cctccagcca
     1861 gatccgcacg gcccctcccg gtgccaatcc gataatcata ttgtcccgat aatacgtttt
     1921 tcccggctta taggtatcag gataggcagt caacatctgc tgccacgtat cccggctgaa
     1981 ccagatctcc gtctcatatg cctttttatc gataaccgaa tcccaacaca ccgtcatcct
     2041 gaccggcagt gccttgccaa tgttaaaact ggctgaaaaa ctgcctacgc gctggctcca
     2101 tgtatccaca ctactctgac ttggtctggc tgcatcaagc tgccgaaata tgctttcccg
     2161 tacatcaccg tcctccagat acaccattgt caccagtgct ggcaggtttt tcgggtaaaa
     2221 aaaattaaac tgccagtggt cataaggcag cttctgcccc cattgatcca taccgtcgcc
     2281 caacagcgtc ttcacgccag ccctacggtc atggcagccg acagccagca ttacgcccac
     2341 tatcacgccc accatcagcg gtaatcgtct cattcctgcc tcctcagaac agtccgtcca
     2401 gcgcatccgc gtttacccgc gcccgctctt cccactgctg ctggtcgtcc atccgccaca
     2461 ccgtccgcac ccagcctttg cccggtgcca tcggccacac gaacgagcgt tcgtcagtcg
     2521 gagcatagtt atttcgcaga aaaacttctc ccgaggacgg actcacccac aggctcaagt
     2581 ctgagtcgat gttccagttc gccgagcaat gcaggtaacg cccgatggtc cgcatatcct
     2641 ccgccgtgaa gccctcaggc tccgcgccac gccggacagc acgcccctga gctatggctt
     2701 tgtcacacag gggaagcagt tctgacggca acgccagctc tttgttactg ctgagtatcg
     2761 gagtaaacac agccccggcg tcttgtgccg catccagcat cacccgcaaa ctcaccttct
     2821 cccagtcgtt cggcacatcc ctgcgcgtca taaacactgc cgcgcccact ttcttctcaa
     2881 aaatagacgc acgcgccttg ttctgattga ccagtggcca cgaaatcatt ttcgtctcca
     2941 cctcaccgtg cggcatcagg tacttcagcg ccggcagtga gtacagcacc tgacgcatct
     3001 tctccgcctt acggtacacc tccgtctgca tttcatccgc gctgtcgcgc gtcacctcaa
     3061 aatccggcat cgtcagcagg gagatttcat cctctcctgc gttatagccc ccgccaatgt
     3121 ccgagtgcgc ccccggcagc gccagctccg gccacatccc cgcgatgctg ttcaggctga
     3181 agttataccg acactcatgc atcgcgctaa tctgaaacac cttctgcgcc actgaaggac
     3241 gcagttcgag attcaccccg ggattgctgc gtccgttaac gtcgtagaag ttcaacaggc
     3301 tcccgatagc cgccactgtg tcaaacagcc ctaaaaagcg cacctctccc gccagtttcc
     3361 cgtggtgccc ggtcaggtct gtccctttgt ctattgccgc cgctatcgcc gcatcctggt
     3421 tcatcacccg gttggcaaaa tgccgtgcag ccgccgcgcc gcggctgaat ccgaacacgt
     3481 caaactgcac cttcgcaatc gccactttgt cacgattaag tttgataaag cgggtcagtt
     3541 ctgcgctgat cttctccagt gcttcatcgg tctttgtcac caccccttca aactgtcgga
     3601 taaggctgct gcccagcgct ttacccagta tcgaatcact ttctccgtct gccgtcccga
     3661 tgccgctgat ataggctttg acctgcgcct cagtctcgct gtcgttaagt tcatcgcttt
     3721 gctgataaag atcgaacagc cgggaaatat tagtgtggcc gccctgccag cttgcattac
     3781 ccagcccagc tctggcgcgc ttttcatact tctcacatgc cctagcccgc tcctcgtcaa
     3841 ccagattggc acagtgggca tatgccagcc gtagatcgtc tgcgttcgcc cggttattcc
     3901 ccgtcccgtc aaagaacacc cccaccgtca gcgttatccc cctgaccggc acccgccgac
     3961 actggatggt tttaaaactg ttgtgtatgc tggttcgatg ggtactgtcc ccaataattt
     4021 cataccggta ataacaggta tcgcccggca catttttttc atcttcttcg accataaaaa
     4081 tccttattta tctgaaagat aaaatcagta gcaaaatcct tcgggacttt tttacatctg
     4141 cctgttagac gtatttacca taatccctta attgtacgca ccgcagaaac gcgctcagcg
     4201 cgattctggc gcaaaaaccg caaaaataga tgttttcccc cgggtaaacc ggaaaaatgc
     4261 gtcaggattg ttttcatggc actgcatgac tatgcatgaa actgaatggc gatcggtttg
     4321 ggcgcgcctg atgcccataa ggcgcatttt cggacgtttt cagccctgat gagaagaaat
     4381 cagacagtag ttacagacga gtcgtgagcg attcaccccg ggagtcatca gcgagtcatc
     4441 cagtattttt cctcgcgact ctctggcgac tcgctttttc tgaacaccag agagacagtg
     4501 tgttgagtca tcgataaatc accgacgact cgttgccgaa tcatccagta gtcgccgaca
     4561 actcgctttt gtataaatct gaataaaaaa atatattttt caaaccataa caacatgatt
     4621 taaaaagcaa atcagaaaaa agttggtttt gcgtgggggg tgggcatccg gggcatgaga
     4681 acagactcgc gtttttctgg agaaactgcg gggatttttg attaaacaat actcaccgca
     4741 gagcggaatt ttatgcaacg ccggctgtgc ggcacaggga tttttaatcc ccagcatgtc
     4801 agagggtgcg gatgacggtt cccgtctggc gttttcatgg gcgccggttg tcaaaaaata
     4861 ccagcgtgat cggtcgaagg ataagcgcag attaatcagt gatgattgag cgcgaatctg
     4921 acaacaagta tagcgacaca gcaacaaaaa agacgccaga aacgcgctca gaggctttgt
     4981 aacgcgattt tttacatgag gcaatggttt cgctttgagg cgttcagccg tgttcagggc
     5041 gcgattctgg tacatacctg actgtaatca tccctacgaa ctactctttg caccagaaac
     5101 gcgcaggagt gttttagcgt gtctccggcg tgatgtcgag ttgtgcggta atgagtgagc
     5161 gaaaaaaatc ccctggatta cagggggaac ccatgatcag actttgaagt ttatttgtta
     5221 tttgatggaa tgagcacgaa tcccggagat gacgttcagg tggcaggaaa cgcgctcaga
     5281 agctttctaa cgcacttttt tgcatctggc aatgggtttc atatgttttg gtgggttcag
     5341 gctgtcagag acgttttgag cgcgtttctg gcgcttgctg ctactgccaa accatcccca
     5401 tggccagttg atgccagaaa cgcgcaggaa ttttgtagcg cgtctccggt ccctgatcaa
     5461 tgaattccct gtcattaaca tgcgtttacc cggaaacgcg ccctgatgtt ctatcgtatc
     5521 gcgggaacgg tgtcgagggt gtcggggcaa cgccctgacc aggtggtaat cgtatagccg
     5581 tgcgtgcgcg gttatacgat tacacatcct gtcccggaat caggattgtt tttcgcgcac
     5641 aactgcggca aattcatccc gttgcggttg caacgatggg tatatcaaaa ctacgaaaat
     5701 tattcaggca tttgctaccc cccttgccct ggcctccacc agtcacgggg gattttttcg
     5761 cctgtcctga ttgtcttttg ccgatggtta cggcagattg gaaaagagcg catttgtggt
     5821 gctctctcca aaataatatt tctcattact caaactctga tggcttcccc ctgtaatcca
     5881 gggggatttt ttaatttatc gctatgcttc gtaattcatt cagaatacag acagtatggt
     5941 taatcagttt ttcatcattt tttcggtgtt tgccctgtcc tgcatcagaa cgctaaaaaa
     6001 acaggattgg aatctcttta tgacgaatcc tccatttcag gattttttat gtaacgtcta
     6061 ctacatgcga tccgttggtg aaccgttagt tatatattgg ttatatgtta gttatatctt
     6121 agttatactt ttgctgcgcg tcagttacag aggcgttttg agggtaaatt ctggaggcac
     6181 gtgaccgact gtatacaaaa tgctggcgca ttttgatacg tcgacgtgcc aaaggggacg
     6241 cccctttgaa accccgccag aattaaaagc ggcctgtctt tttacagtat tcagctctgg
     6301 ctatacacca acctatattt tctcctttgg cataatgaag cgcatcattc cattttttcc
     6361 atgctccaat gtaatcacct tcacgctcca gtctgacagc ttcgtttgcg ggtgaaaaat
     6421 atttattttc taaataaatt gctttgtcct gattcatatt cgtaatttat atttttattt
     6481 gaattaaaaa agagcgagcc catcgggtta atacatattg gcatgactgt tgatatatca
     6541 gtccaacagc acgctcgtaa tcgttttaac gtgattttcg caacatggca atccgggttc
     6601 atctgtccgg taatcaataa aaaccgtgcg aaaaacggga caggatgtgg ccattaatgc
     6661 taatgtatgc atacattagc attaatggcc ctgacaaata ccaggcatcc ggtctcagga
     6721 tgccttgatt cttgcctggt agatgcgctt tacccgttca atttctgcca gcgttgcgga
     6781 ccagtgtagt tcggcggcat gacgatatct ggcagccatt ttttccacca tctccggatt
     6841 gctgtccgcg ctgcggtgct gacgcccctt ctccccaaaa acttcaacaa ccagccactg
     6901 gcttcctgtt ctgccgaaca atgccttgca caaacgtctc acagtcagcg gggttaaatc
     6961 tggatcggga tcgtaggcag tggtagcaaa aataaagcgc agcatttctg atgtcttgta
     7021 acgctttacg gcagctgcca gacgtgcggc gcgttggggt gcttcccagc ggtcgtcact
     7081 gtaattagca acttcagcca gataatcctt ttcaaggttt tcagccgtgt aacacctgcc
     7141 gcctaaaaaa aaccgcatgg cctccctggt tttgtctctc acatatcatc cctcattaat
     7201 gctaatgtat gcatacatta gcattaatat ctgacagcaa caagcggcga agactacccc
     7261 taatcaagta catttcttgc attgtactat ttcatgccct acaatgtagt aacgtaccta
     7321 tatacatcaa aaggcaacag catgaagatt acaaccctat ccagtcggga acttaatcaa
     7381 gatgtaactc gagccaaacg cgccacgaat tatggtccag tatttattac tgatcggggt
     7441 aaaaccgcac atgtacttct tagtatcgaa gaataccgaa aattgacgca acaacgacga
     7501 actctcattg acattctggc gatgcccgat gctgatgcag ttgaatttga accgcctcgt
     7561 attacggtta gtttaaaacc tgcggagttt ttatagtgta tattcttgat actaatgtta
     7621 tctctgagct acggaaggct cggacaggac gtatagataa taatgttgca aaatgggcat
     7681 tgcatacaga tgcggcagat ttctttattt ctgttatttc cataatggaa cttgaacagg
     7741 gaatattaca agttgaacgt cgagatatgc gacaagggat tatgctacgt tcttggatgg
     7801 atcagcatgt gttaccagcg ttttctgata agatacttcc tgtagatact gcggttgcat
     7861 tacgttgttc tcgtttacat gttcccgaca ggcgcagtga acgagacgca ttgatcgcag
     7921 caacggcgct ggtacatggg atgacggttg taaccagaaa tgttggtgat tttgaattaa
     7981 ccggagtatc aattattaat ccctgggctt gatatcgctt cccttcttct aggggtccgc
     8041 ttggaaaacg gaaattttcc cacgctaaga gaattgagtt gaacgagtgg ttacatccaa
     8101 gccagctaac tcactgaacg taaaggctgt acgaaatgtt ccgaccgtcc ggattcctga
     8161 cagtcatttg gctaaaaaat agtcttaacg tggtttattt tccgaattcc aggctgaccc
     8221 catcaaagca tccttcagtt cagcgtcgat tcttaaactc atattaacct gtgccattca
     8281 tcacctcgtc attaccacgt atatacacag tatataatta ttcagatatc gtcaccatga
     8341 tatgccgcgc cagcaacatg gaaggcggca ctccatgctg tatatggcag aggagtaaaa
     8401 ccgccgaatc ccggcgtaag ccggtacagt cgtccgaatg tcagtagttc tactcccccc
     8461 tgcgaggcca gtgctgacgc gggacatgga acgggtgctg gagcgaagtg gggaagctcc
     8521 ggtatactgg ccaaacctac acgcctcctt ccgtctccgg gaagatgctg gcttcgtacc
     8581 ctgcgtgcgt ctgatctgta gctggccgtg gcacggctac ggacagattc tgctccgcaa
     8641 cagtatcggc gtgcctgacc tcctgaaagt cccgtaacgc attttctcac ctcccatgcc
     8701 ctgacgctat tgagcgcgtt acagaacaat ttaagtcatt taacgagcct ccaacggctc
     8761 gatcgaaata aatcaaatcg taatcgtcaa cgatatatga gaggagaccc cgctcgccgc
     8821 aaatcgatga ccgaacgtag tgagcgaatc gacgaggaag cggaagagaa cctgtacgaa
     8881 aatggagcaa ttacgcccca ccgtcaagaa atgagctggc tctccacggc gcgccatcat
     8941 agacagatgt ggctctgcaa ctgcgccatg cgatcggaga ccgaccagta attatcttta
     9001 atgcccggtt cgacacccgc attctgaaac agactgctgc cgcacatagc gatccggctg
     9061 actggctgga agaactgaca gtatattgtg cgatggaact ggcagcagga tattatgggg
     9121 ccaccaaccg ctatggcacg atttctctgg cctgtgctgc cagcaggccg gactgacatg
     9181 ggaagggctg gcacactcag ctgatcgccg acacacggat gacgtcaggg gtggtaaacg
     9241 ctattactgc atatcatcag gaattgctgc agcttatgta ctgcgataac cctgaactca
     9301 actatgcaaa cctgaaatcc ctgcaaaaac agctggatga aatgtatacc tgcatccgta
     9361 ataatcagat gatgctccca aactacggaa aaatgcgccg gtatggtgaa ccggtttcaa
     9421 caacgtttgt agagtcgaca atcaacgaag tgatcgccag acgatgaccc aaaagcaaca
     9481 gatgcagtgg ggtaaggaag gtacgcatta tttgttacag accagaaccg ctgtcctgaa
     9541 taattaactg caggacaaat tcgccagttg gtacccgggt tgcacgatag acgctcagag
     9601 tggaggaaag ttatccgcca tggcagcata actacttccc cagttttttc agctctccct
     9661 taccaatatc ctacgacgat cacatcgaaa aaaaacacgg aacaggcgaa cagaaatata
     9721 attaattatt tgagtttaaa ggaaaaaaga ggatacaagc tttttgttgt atttttattt
     9781 gtataaaccg acaaaaaaga ggccatactt cccgctttct ttgtctgttt acacggaaca
     9841 tttcaccact ggatacgcta atagatcgga tatgagcaca tattgcgccc gcctttcatg
     9901 cagagcataa aatgaacaag attcacgcta attattacct gcaagattct accgcacgcc
     9961 agatagttca acgcacaatg gggattattc cgtactcggt aaatgtgatg aacgagcatg
    10021 ggatgatcat cgcttccggg gaagcctcac gtttacatca acgccatgaa ggtgcggtac
    10081 tcgcattgac cgaaaaccgg gtcgtcgaaa ttgattccgc gaccgccaat aagcttaaag
    10141 gcgttaagcc cggaatcaat ttaccgatta attttcgcga ccagctgatt ggtgtactgg
    10201 gcattaccgg cgagcctggt gaagtgaagg catatgcaca gttggtgaag atggcttcgg
    10261 aattaatcat tgaacaaatg gctctcctcg agcaaaaaca gtgggataag cgttaccgcg
    10321 aagagctgat taatcagctg attttacgcg atccgccacc ggactcactg aatgcggtgg
    10381 tctcatggtt gggaattaat ctccagcttc cgcgggtggt ggtgattatg gaactgcaac
    10441 aacctgatcg cgacgcactg cataatctga tggagtattt tgaatatcgc gcccgcgacc
    10501 atttggtcac gttttccgac tttaatgatc tgataatctt aaaacctatc tcgctaaaaa
    10561 acggcgtctg ggataaatcg ttggaattaa gcgatctgca gagacttaaa cattacgccc
    10621 agtcctgtgg tttttcccga ctgattgtcg gcggctattt ctctggagaa aacggcctgc
    10681 atcgctcttt tcagaccgcg aaatccaccc agtccatggc gaaaagactg aagttgaagc
    10741 atcagttcat ttttcatgac gactatgcgc tggcatcatt attagatggg ctttctggta
    10801 cctggcaagc ggaagaatta tccaaaatct ggcgcaatct ggttgccaag gacaccaaag
    10861 gtatgctgca aaaaacgctg cagaattatt ttgatcataa ttgtgatctt tcgcaaacag
    10921 cgactggctt gcatattcat gtcaatacat tacgctatcg actgcagaga gtggaagata
    10981 ttacaggaat acaaatcaat aacttaaagc agttattctg gctgtatatc ggcatggagt
    11041 tacaacgctg aactgtattt ccctccaaat ttttcgccgt ctgacgtcgt atttttagcc
    11101 gatacacaca agtctaacgc ggctgctttc tccatactgc cccctgttat tttcatggag
    11161 aaagcagaag atggtttcaa taacagcaac tggcgccatc atcgcactcg tggtggcgat
    11221 cgttttaatt ttacgcaaag tctcgccggc ttacggcatg attgtcggtg cgctggtagg
    11281 cggtctggtc ggcggcgccg atctggtgca aaccgtgacg ctaatggtca ctggcgcgca
    11341 aggcattacc aatgccgtgc tgcgcattct cgcagcaggt gtactggccg gcgtgttgat
    11401 agaatccggc gcggcaaaca ccatcgctga aactgtggtg aaaaaagtgg gggaaagccg
    11461 gacgctttta gccttagcta ttgcgacgat gattctgacc gccgtcggag tatttatcga
    11521 cgtggcggtt atcactgttg cgccgattgc cctgtctatt gcccgtcgtg ccggtttgtc
    11581 gaaactcgcc attctgctgg cgatgattgg cggtggtaaa gcgggtaatg ttatgtcgcc
    11641 taaccccaat gctatcgccg tttcggacac ctttcatgtt cccctcacct ccgtgatgat
    11701 gaccgggata atccccggtc tgttcggcct gatcgtggct tatttcctgg caaaatgtct
    11761 cagccagaaa ggcctgatgg tgatgcccga cgaaatgatt tcacacgaca accacggcaa
    11821 gcagccggga tttcttgccg ctatcagcgc gccgctcgtc gccattgtgc tgctggcgct
    11881 acgacctgtt gcgggtatcg tcatcgatcc gctgattgcc ctgcccgtcg gtgggttagc
    11941 cggtgcgatc cttatgggca ggtttaagca aagtaacgcc tttatgatgt ccgggcttac
    12001 gcgcatggcg ccggtagcca tcatgctgct cggtaccggt acgctcgccg ggattatcgc
    12061 caactcggaa ctgaaagatg tgctcattca tggactgacc tcttccggtc tgccttctta
    12121 tcttctggcc cctgtttccg gcgcgatcat gtccatggca acagcctcca ccacggcggg
    12181 aaccgctgtt gccgccgggg tattcagcca gacgctgctg gggcttggcg tatcagccct
    12241 tgccggttcc gccatgattc atgctggcgc aaccgtactt gatcacctgc ctcacggcag
    12301 cttcttccac gccacgggcg gcagtgtcgg catgctgatc catgaacggt taaaactgct
    12361 gccttatgaa acccttgtcg gttttgtggt cgccctgatt tccacactga tgtttggcgt
    12421 cttcggactt gccgggtaaa gacacgatga aaatagtcat tgcaccagat tcatttaaag
    12481 aaagtttatc ggccatgcag gtggccaccg ctattgagca gggatttcgt gaaatttatc
    12541 ccgaggccga gtatgtcaaa ttgccgatgg ctgacggcgg tgagggtacc gtcgaatcaa
    12601 tggttgaagc aaccgaaggc cattatctgt tccagaatgt caccggaccg ctggggacgc
    12661 cagtacaggc gcgctggggt atgctgggag atgggaaaac ggcgatcgtt gaaatggcct
    12721 ccgcatccgg actacatcat gttcctccag aatgccgtaa ccctatgctt accaccagtt
    12781 atggcacagg cgaactgatc cttgccgcac tggaccacgg cgttgagcga attattctcg
    12841 ggattggcgg tagcgccacc aatgacggcg gcgcaggtat gctacaggca cttggtgtgg
    12901 cgatgcttga tacaaacggg aaatcgcttc ctttcggcgg cggcgcgcta agcaacctgg
    12961 cgctcatcga tgcctcaggc tgcgacgctc gactgaaaaa cgtttctgtc accgtcgcct
    13021 gcgacgttaa caatccactc tgtggcgcaa caggagcgtc agccatcttc ggcccgcaaa
    13081 aaggagcaac accagaaatg gttgcctcct tagaagctgc gctgaccaac ttcggtatac
    13141 gacttcagga cgcaaccgga cgaaacgtga tgacgacgcc tggcgcaggt gccgctggcg
    13201 gcatgggcgc ggcgctgtta gggattctga atgcggagtt aaagccggga gtggagattg
    13261 ttattgacgc gctgcatctc gatagcctgg tcgcggatgc cagcctggta atcaccggtg
    13321 aaggtcgtct tgatagccag agcatcagtg gtaaaacacc gattggtgtc gcacgctgcg
    13381 ctaagcggta tcagaaaccg gtgatcgcac tggcgggcgg cttaacgacc gaccaccaga
    13441 tagtgcatga gcacgggctg gacgcggtgt tctccgtgct gacgcaaatc gccactctgc
    13501 cggaagcgct acaggatgcg gaatacaacc tccgcgtttc ggcacgcaat attgccagcg
    13561 tctggaaatt atcacagcag cagatgcgct aatcttcagt caggccggca agcagtagct
    13621 ggcctggata ttctttctca cctggcccat gctaaaacag cctgcttcag tcatcccacg
    13681 caaggcacta gcatggcaaa aaaagagggg agtagtggat cccaaaagac ggttaagcgg
    13741 ttaaattgaa tggtcaataa gggggctgtt tgggaaacga atctatggtc actcccgttt
    13801 ttgcaacacc gattttgacg ataagtgggc ttgcttgaat ctatccggcg tctgaatggg
    13861 attttattcc cgcgcctcga tgagttccgc gcctgatgaa cctcaagaaa atatacggct
    13921 tcaatgagcc tttccgtttc acaggttcct caacaggccg gtgggccgtt catatcatca
    13981 ataccgtgat gctgatactc cgtacccacc ggaaccgcac ctgatgcggt tccggcttaa
    14041 gggctgtcag gactcgcttt tggctgccat caacaaaccg gaagagatgc cggattatct
    14101 cttccgtcaa ctcggtttta atcagacctg gcatgagtgg aagcgggacg aacagcacag
    14161 gcaacaacaa cgccggcccg gacactaccg gggcatgagt atgtaatgtc agggacagct
    14221 atacgccata aaaaaatgaa atagatggcc tctctataaa gaggccatga tatttactaa
    14281 ccctgttcgt taatacattc tataaagttt ttatgtaggc tatctatatt ttctcgggta
    14341 tgaatagctg atattgcaca acgtagaatt gcctttccct cttccactgt aggatacata
    14401 gcaacggttg tagcaaaacc acgatcatgc atttttttgc atatcgttat agctttttct
    14461 tcattgccaa ttaacaatcc acgtattggc attgtagtcc ctttgtttat gacttcggtt
    14521 tcattaaatt tgtcatcaaa gtattttata acttgttgta agtcattttg acgcaaggat
    14581 actgtgccat cgaggtgtaa atctgctgct gcgatacatg cagcaattcc tggtaatgat
    14641 ggtggaccac caaaagcata ggtagttcca tacgttttta taaagtttat ggtgtcagga
    14701 taattgcatg caataaatcc accatgagag ccaaacgctt tagataatga accagcgata
    14761 ataattctgt catcaagttt gttatccaga caagacatta catatccgga gccatttttc
    14821 ccatatattg acatgccatg agcatcatca atgtaagcat acccatcaaa tctgttagcc
    14881 attctaacaa taaattcgac aggagccacc ccacccatcg aaccgacact atcagatata
    14941 gatattggta ccatattatt tattgaagca tatttaaacg cttcttctat atcattttta
    15001 tgttgaaagt caaccctgat tgtttcaccg aactgttgta atagtcctct atttatttgc
    15061 aatgttgcat gcgctgtttt ctctaaaata aaatatggtt ttttattaaa cttgtaatta
    15121 ggtagtgttc ctgatgcaag aagtgggagt acagctaaat ggcatggagt aaccgcatta
    15181 aatgttaatg tgtgcgcccc attcattatt ttatttaatc tcttttccaa ttgctcgaat
    15241 aaatctactt ttacgcgcgt tctggcgacc gaaagttgga ctccaaatct ttcaattgca
    15301 ttagcgattg catgttttag attttcatgt gtttccagtc caagataaga gcatgacaca
    15361 aactcaatca tctccctccc atcattaaca gtgattacct taccatttct tctggtgatc
    15421 actagttcat ttatatgttc gttaaatact cttgtttggt
//
LOCUS       DAZFRN010000120        15317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFRN010000120 DAZFRN010000000
VERSION     DAZFRN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.122"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            206..2125
                     /gene="ygfT"
                     /locus_tag="ACHKUB_003600"
     CDS             206..2125
                     /gene="ygfT"
                     /locus_tag="ACHKUB_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO6656563.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            2125..2613
                     /locus_tag="ACHKUB_003601"
     CDS             2125..2613
                     /locus_tag="ACHKUB_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6656564.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(2649..4016)
                     /gene="ghxQ"
                     /locus_tag="ACHKUB_003602"
                     /gene_synonym="ygfQ"
     CDS             complement(2649..4016)
                     /gene="ghxQ"
                     /locus_tag="ACHKUB_003602"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO6656565.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(4052..5368)
                     /gene="guaD"
                     /locus_tag="ACHKUB_003603"
     CDS             complement(4052..5368)
                     /gene="guaD"
                     /locus_tag="ACHKUB_003603"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO6656566.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(5386..6786)
                     /gene="xanQ"
                     /locus_tag="ACHKUB_003604"
     CDS             complement(5386..6786)
                     /gene="xanQ"
                     /locus_tag="ACHKUB_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO6656567.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(6951..9821)
                     /locus_tag="ACHKUB_003605"
     CDS             complement(6951..9821)
                     /locus_tag="ACHKUB_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO6656568.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITYL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(9818..10597)
                     /gene="ygfM"
                     /locus_tag="ACHKUB_003606"
     CDS             complement(9818..10597)
                     /gene="ygfM"
                     /locus_tag="ACHKUB_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO6656569.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(10648..11976)
                     /gene="ssnA"
                     /locus_tag="ACHKUB_003607"
     CDS             complement(10648..11976)
                     /gene="ssnA"
                     /locus_tag="ACHKUB_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO6656570.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(11979..15077)
                     /gene="ygfK"
                     /locus_tag="ACHKUB_003608"
     CDS             complement(11979..15077)
                     /gene="ygfK"
                     /locus_tag="ACHKUB_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO6656571.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PSMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQHNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFDNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         3747 a         3916 c         3982 g         3672 t
ORIGIN      
        1 cgcactctcg catttaatcg tttttatctg gatagcgctc ttttggtcgg caatgttgaa
       61 ataatgaaag cggcatagat tgaggggcaa taatttgaac tcagtcgcaa aattataatt
      121 aaaacggtca tgctacctaa tatatgcccg tttattgcat cgcgaaattt gagaagacat
      181 aactcattaa atgaaaggga tgcaaatgaa taagtttatc gctgctgaag ctgcggaatg
      241 tataggctgt catgcttgtg aaattgcctg tgcggtggca cacaatcaag aaaactggcc
      301 gctgagtcac agtgactttc gaccgcgtat ccacgttgta gggaaaggcc aggctgcgaa
      361 tccggtggcc tgccatcact gcaacaatgc cccttgtgtt acggcttgtc cggttaatgc
      421 tctgactttc cagtccgata gcgtacaact ggacgagcaa aaatgtattg gctgtaaaag
      481 atgcgcaatc gcttgcccct ttggcgtcgt tgagatggtc gatacgattg cgcagaaatg
      541 cgacctttgt aaccagcgca gttccggcac gcaagcctgt attgaagtct gcccaacaca
      601 ggcgttacga ctgatggacg ataaagggtt acagtggata aaggtggctc gccagcgcaa
      661 aacggcagca ggaaaagcgt catcagacgc tcagccatct cgcagtgcag cgttgctccc
      721 cgttaactcg cgtaaaggcg cagataaaat ttcagcgagt gaacggaaaa cccactttgg
      781 cgaaatctat tgcgggctgg atccactgca agcgacttat gagagtgacc gctgtgttta
      841 ttgtgccgaa aaagctaact gcaactggca ttgcccgctg cataacgcta ttccggatta
      901 catccgtctg gtacaggaag gaaagattat tgaagcggca gaactttgcc accagaccag
      961 ttccttacca gaaatctgcg gcagggtatg cccacaggac cgtctttgtg aaggcgcatg
     1021 tactttgaaa gatcactctg gcgcagtctc tatcggtaat ctggaacgct acatcaccga
     1081 taccgcgctg gcgatgggct ggcgtcccga tgtcagcaaa gttgttcccc gtagcgaaaa
     1141 agtggcggtg attggcgctg ggccagcagg gttagggtgt gctgatattc tggcgcgcgc
     1201 gggagttcag gttgatgtct ttgatcgcca tccagaaatt ggcggtatgc tgacttttgg
     1261 cattcctcct ttcaaactcg ataaaacggt attaagccag cggcgagaga tattcaccgc
     1321 aatgggaatc gactttcatc ttaactgtga aattggccgc gatattacct ttagcgattt
     1381 aacttctgaa tatgatgcag ttttcatcgg cgtggggact tacgggatga tgcgagcaga
     1441 tctgccgcat gaagatgcac ccggtgtcat tcaggctcta ccgttcctga ctgcccatac
     1501 ccgccagctc atgggattgc cggagtctga agagtatccg ctgacggacg tggaaggtaa
     1561 gcgagtcgtg gtattgggcg gtggcgatac gacaatggat tgtttgcgga cttccattcg
     1621 cctcaatgct gccagcgtga cctgcgcgta tcgtcgtgat gaagtcagta tgccgggctc
     1681 gcgcaaagag gtggttaatg cgcgcgagga aggtgtcgag tttcagttca atgttcaacc
     1741 gcaatatatc gcttgtgacg aagatgggcg tttaactgcg gtgggcctga ttcgtaccgc
     1801 catgggtgag ccggggccgg atggtcgccg tcgtcctcgt ccggtagcgg gttcagagtt
     1861 tgaattgccc gccgatgttc tgattatggc ctttggtttc caggcgcatg ccatgccgtg
     1921 gttgcagggc agcggaatta aactcgataa atggggcctg attcaaaccg gtgacgtcgg
     1981 gtatttacct acccagacgc atctgaaaaa agtctttgct ggtggtgatg cagttcatgg
     2041 cgcggatctg gttgtcactg caatggccgc aggaaggcag gcggcgcgcg atatgttaac
     2101 tctgtttgat acgaaggcat cgtgatgaaa tcgttaatta tcgttaatcc ggctgactgt
     2161 attggctgcc gtacttgtga agtagcctgt gtggtcgctc acccttcaga acaggagttg
     2221 aatgccgatg tctttttacc ccggctgaag gtacagcgac tggacagcat cagtgcgccg
     2281 gtgatgtgcc atcagtgtga aaacgcccct tgtgttggcg cttgccccgt gggggcgctg
     2341 acgatgggcg agcaggtggt gcagaccaat tctgcccgtt gtattggctg tcagagttgc
     2401 gtcagtgcgt gcccgtttgg aatgatcacc attcagtcgt tgccggggga tacccgacaa
     2461 caaattgtga aatgcgatct ctgtgaacag cgggaagagg ggcctgcctg cgttgaatct
     2521 tgcccaacac aggcgctgca gttgcttact gaaagagaac tcaggcgagt ccgccagcag
     2581 cgtattgttg ccagcggtga gaatcctctg tgaaagaaat ctccgcgctg aaaggcgcgg
     2641 agaatctatt agattgccca accacccgcg taaaatgcga ccagtgcggc agtaatgatc
     2701 accgtaccaa tattcagctt ttgccattcg cgtgcaaaga cgcggcctac gaccagtgtc
     2761 acaaagccca gcataatacc ggtaacgata ttacaagtca gaacgatgaa cacggcacac
     2821 accaggccag ccatagcgtc aataaaatca ttgaaatcca gcttcgagac gttacttaac
     2881 atcagcaaac ctacgtacat cagagcgggt gcagtggcgt aaccagggat caaaaatgac
     2941 agcggtgata agaacagaat cagcaggaat aacgccccca ctacggttgc ggttaaccct
     3001 gttttacccc cggcggcggt tcctgccgct gattcgatat aaaccgctgc gggcgctgcg
     3061 cccaccaggc cggagaatat tgaacttact gagtcactgg tcagggcttt gccgccgttg
     3121 atgatctggt tgtctttatc caacaaattc gcctgaccgg cgacggcacg gatggtgcca
     3181 gtggcgtcga acactgcggt catcaccaat gccagtacac tcggaagtac ggttggctgg
     3241 agtgcgccca taatatcgag gctgaaaatc agagacttac catcttcgcc agtcagactt
     3301 ggcatcgcca ccagaccgtg gtatttcacc gctgggtcaa agattaagcc gataatcgaa
     3361 attgcgataa tcaccaacaa gatcccgccg ggtacgcggc acttctccag accgaagatg
     3421 accgccagcc ccagcaagct catcatcacc gggaaggagg taaacgcacc gagcgccact
     3481 ggcaagcctt caatcgggtt tttgataacc atacccacac cgttagcagc aatcagcagc
     3541 agaaacaggc cgataccgat acctgtaccg tgagcgatac ccatcggtaa attacgtaag
     3601 atccaggtac gcacaccggt tacggaaata gcggtgaaga tgacgcccat cagaaagacc
     3661 gcgcccagtg cgacaggaac gctaatttgt tgcccgagta ccagactgaa tgcggtaaat
     3721 gccgtcaagg aaatcgcaca accaatcgcc attggcaaat tggcccacaa ccccatcagc
     3781 aacgagccga agcccgcgac cagacaggtg gcaacaaaca cagctgcggg aggaaaacct
     3841 gctttgccca gcattcccgg aacgacgata acggaataaa ccatggccag aaaggtcgtt
     3901 aagccagcca gtacttcctg acgaacggta ctgccacgag cggtaatttt aaaataatta
     3961 tccagcgcgc cctgtggctt tggtgcgtcc ggtgtttgta ggatgtctcc agacataatg
     4021 atgtcctcac gaggatgtta aagttttatt attagttgcg ttcgtacacc agacgaccat
     4081 caacgtaggt gcggtagatc gaacggtcat cgcccaacgt catcatcacg aacaatttgt
     4141 cgactaaaga aacagagttg tcatagcgca gctgctgtag cggagtggcg gtgggttcca
     4201 tcaccacgaa atcagcctct ttgccaggta aaaagttgcc aatcaaatcg tcaaggccca
     4261 gagatttcgc tccgccgagc gtggccaggt aaaacgcttc atatgccgag aggcgatagc
     4321 cttgtaattg caatactttg taggcttcgt tcagcgtttg cagcatgttg aaagtggttc
     4381 cggcaccgat atccgttccc atgcccactt taactttctt ctgccatgct tttttcaagt
     4441 tgaataagcc gctgccgagg taaaggttgg aggtcggaca gaaagcaatg ctggatttgg
     4501 tttcgctgag acgatcccac tctttttctt cgagatggac gcagtgagca aagacacagt
     4561 ttttaccggt caggccgtac tgatggtaaa catccagata accatcatgg tcaggataaa
     4621 gcgatttcac ccaggcaatt tcatctttgt tttcacagag atgggtatgt acccacgtat
     4681 ccggatattc ttctttcagg cgttgcgcca tcgccatctg ttcaggagat gaggtcgggg
     4741 cgaagcgtgg cgtaatcgca tatagcagac gaccattttt gtgccagcgt tcaatcagtt
     4801 ctttgctttg gtgatagctg ctttcggcag tgtcgagcag ataatccggt gcgttgcggt
     4861 ccatcatcac cttaccggca atcatacgca tattgatatg actggcggct tcaaacagcg
     4921 catcaacaga ttgcggatga acagtgccaa acaccagcgc cgtggtggtt ccgttacgta
     4981 aaagctgctt gatgaagaat gccgacattt cgcgggcgta ctctaaatcc tcataacgac
     5041 gttcagtagg gaaggtgtgt ttattcaacc actccagcaa ttgttcacca taggccccca
     5101 ccatttcact ttgcggataa tggatatgtg tatcgacaaa gcccggtact atcagtttgc
     5161 cgcgatagtc gcgcacgcga atagtgtcag gaatttgatg ctttccgtct tcccattcgc
     5221 caaaccattc cacttttccc tgtttaatga gtaataaacc atcctcaata aaccgcagcg
     5281 cagaggcaat ctcttccggg ttatcgaccg tacgggtgac atcaataaaa ctgcctcgta
     5341 ccgctttcaa cgtgtgttct cctgacatca ttgactcctt tactgttaat ccatctcttc
     5401 cgctgaggta ataccaggca gaacgttttc ctgtcggtag ccaccaggga ggataatatt
     5461 taataaaatc gcagttaacc cgccagcaca aatagggttt tcaactagta cataaataga
     5521 ggctggcaat attttaaaaa tttcgggatc gtaggagacg ccaagtccta aacctaaaga
     5581 agtggcgaca ataagtgttt ctcggcgctt taaaccgttg gtgatgatta tgcgaatccc
     5641 agcgatggca atcatggaaa acatcaacgt cattgcgcct cccagaactg ccgagggaat
     5701 ggtcgtgaag aagccgccaa tcatcggaaa caaaccgagg ataaccagca ttaccgcgat
     5761 ggttcgcccg acataacgtg aagcgacgcc agtcatctga ataaccccat tattttgcgc
     5821 aaacgtggtt aagggtaatg acccgacagc ggaggcgata acagaaacca gaccgtctgc
     5881 cagcacgccg cctttcagtc gggactgata ctcttctccc tgaatgggtc ggcgggaaac
     5941 cattgccgtg gcggtgatat cgccgacagc ttccagcacg ctaagcagat aaatcgtgcc
     6001 aaccaccagg aactgatgga agctaaaact aaagccgtat ttgaacggat gcgggatggt
     6061 gattaacggc aaattgcgca tactgctgaa atccaccatg cccaggcata acgatgcgat
     6121 atagccgaca catagcccaa tggcgatccc tcccatgcgt agcaacggac tgcgacagca
     6181 gttaaagccg atcaccacaa ttaaaaccaa taaaccaacg ccgagatgtt cgtaattgcc
     6241 gaatgtaccg ctgcttttgg ctgcaaatcc tccaccaaaa tcgataatgc cgactttaat
     6301 caggcttaag ccgatcatca gtaccacaat accgctgacg gtaggcgtaa taacccgacg
     6361 taaatagggc aagataaatg aagatccgac aaccagaaat gcgccaacga aggagacgcc
     6421 gagaagcgac gacatgatta actcttcgtg aaaaccgtcg cttttcatgc tgctgcccag
     6481 cgcaatcatg accgtaacaa atgaaaaatt gactgactga attgagagta ggccagaacc
     6541 gacgatgccg tagcggttta cttgtaacca ggtaccaata ccagaggcga tcatcgccat
     6601 agaaacaaga taggcagttg tttcagcgga aagctgtaag gccgccccca cgattaacgc
     6661 gggggttacc atcggaacga aaattgccaa cagatgggta atggcaccaa cgagagcctg
     6721 atgaaaggga gggcgatcct ccagttcaaa tataaggtca gaacctgcat tgtttatatc
     6781 agacatccct tcactccttt taaagtaaat ttgatcatct gaattagtaa aataaattcc
     6841 cacgaatttg gggcgcagga cgccttgaga acggcttatc tcattttcgc agtcactatt
     6901 tagtcatcgt tcaaacagag aaacaggccg ccgaagcggc catttattac ttatattttt
     6961 tccagcgcgg tgagtatttt ctccggtgtg aaatgccatt cgcgtaacca gatgccgcag
     7021 gcatcgtgaa ttgcggtagc aatcgccgga gctgcgccat ttacaccaat ttccgagatc
     7081 gatttcgccc cgaacgggcc gactttatcg tcgctcggca ccaacacagc gcggaaatca
     7141 cgcggaatgt caccaatttt cggtgcgccg taactgcgta aatcacgcgt taacgggtga
     7201 ccttcggcgt tataaatgat ctcttcgctc atactgtggc cgatagcacg cagggtggca
     7261 ccgtagattt gtcccaacgc taactctgga ttgaccggtg taccgcagtc cagcaaggcg
     7321 tagaatttat ccaggcggat ttcacccgta cgcgtgttga cggcaacttc agcgaagttt
     7381 gcgccatacg ggaaagcgaa atcaggcgtg atataactgc cagttcccac cagtgaacca
     7441 aagccggtgc cggtttcgcc tttatgggca atatccccga aactaacttc gcctttcttg
     7501 ccgcgcacga cgcccggcgt tgctagttga acatctgcca ctggctcacc caacatttgc
     7561 gcgccgtgga acagaatttt ctcccgcaga ttttccgctg ccaaacgcgc cgcgttaccc
     7621 gagaagcaag taccggacga ggcatatgcg cctttatcaa acaacgcatg atcggtatca
     7681 ccggagataa catgcacgtc ctgcggtggg cagtgcagca cttctgctgc cagtttcgtc
     7741 actacagtat ccagaccagt accaatatcc gcaccgccag aatgaacgat aaaggtgccg
     7801 tccgattcca gtttgatcat gcagttagcc tgatcgatat ccgggatacc cgatttctgc
     7861 atgataatgg cgacgccgcg accgatgtgc cagtcaccat tttgcggttt cggtgaagac
     7921 cattggatca tctcgcgacc ctgacgcagg atctcttcca gtgcgcagct ggcggcggaa
     7981 ggaacggagg tcggcgcttt accttcaccg attgcaccga gaattttcag ctcttgccct
     8041 tcgtgtaccc ggttacgttc gataatttcc agttggtcga tctgtaactg ttcagccagt
     8101 tccgctaatg ccatggtgat agcgaagtta cctttcggtg cgccataacc ctgataagca
     8161 ccatttgggc aaatgttgct gtagtaggtg gtgacctgga aatcgacgtt atcgcacgga
     8221 tataacggca gcgacagcgc cggtccgtta cacggtacgg tgagtgagtg gttgccgtaa
     8281 gggccagtgt tggcgcggaa atccatcttc actgccgtca ggcgaccatc ttttttcgct
     8341 cccagtttga cggtgacttt cgcgacgtga cgagaggtgt tagcaataaa ctcttcttca
     8401 cgggtgtagc ggaacagtac cggacgcccg gtcacgcagg ttgcccatgc gcacacttct
     8461 tccagcagga tgtcctgttt ggagccaaaa ccgccgccaa ctcgctcttt aatgacatga
     8521 actttatgct gtttcatgcc tacgaggcgc gcgacctggc ggcgtaagtg ccatggtacc
     8581 tgggtggagg cgtggataac cagacgatcg ccgtccatcc gggtaaagca gatatgtgtt
     8641 tcagtcgggc actgctgtgc ctgcgttgag ttataggttc gctcaatgat cacatcggca
     8701 tcggcaaagc ctttgtccat atcgccaata tgaccatgaa tactggcggc gatatttttg
     8761 cgagggcgag aaccgatcgg gaagttgatg atcatatgct cgccgcgctg ggctgcatgg
     8821 ctgttatcgt cttccagagt atctggcgca ccagcaacat acaccaccgg ttcatcgtgc
     8881 acgacaggcg catcttccgc cattgcttcg tcgatcgaca ttaccggctt aagcacttca
     8941 tattcaacgt cgatgagctt caatgcttcg agcgcaattt cttcactttc tgcgacgacc
     9001 gcagcaacac gatcgccgac gtgacgcatt ttcttgccga acatacggcg gtcaagcggt
     9061 gacggttccg gtgcgctctg accacccggg gtatagtaga tatccgggca attcaggtga
     9121 gtaataacgt gaacgacgcc cggtaaggct tcagctttgc tgacatccag ataagtaatc
     9181 agtgcgtgag cgtgtgggct acgtaacatt ttaatgacgc aggcgtcagc cgttacgcgg
     9241 tcttcaacat agcagggttt tgcctgcacc attttcgcgg catcagtttt aggataatgc
     9301 ttgccaatga cttcaaggtc gtcacggaaa gtcggagcga tatcaatggt ggcctgcgga
     9361 ttatttttac gtgcaaccgc cagttcaatg acctgatagt attgttgcca gcctgcatcg
     9421 cggctgaaca aaccagaaag cgcgtcgtcg atctcttcgc gggtaggtgc ggcgatgcga
     9481 tcgagaagat cggtgataat cagagctgca gctggatcgt tataaccaga ctgcaccacg
     9541 ccaacatcaa ccattgcctg ttgaaccaga cttaactcgt tccatttgcc caaagattct
     9601 gcggtacgaa tatctgcctt ctctaactgt gcggcaataa gcaaggacgc gttaacgata
     9661 ttaccgttaa agattattgc gtcagaaccg gcaaacccga agccatcatc actgttgcgt
     9721 acagagtgca ttcccatgtt aaacaacagc ttttgcacgt tttcgcctgg attaacggtt
     9781 agctcctgag gcgcgccatt taaagtaaag tggatgatca tacggcttcc tccccagcct
     9841 gttggcagtc ggcatacagg tcggctacca gaactcccgt gatatagcgt ttataggcca
     9901 cgctgccccg caaatcttcc tgcgggaaaa tggcgttggc gacagcctgt tcaagtgcat
     9961 tgccttccag attttgtttt tcgacatcat gcagacgcag tgctttactg gcgacgccat
    10021 ccagcgcaat tcgcataccg tcgtggtctg tcattgcaac ggctgccgtc acgacggtta
    10081 aacctgcctg agaacggcta attttgcggg tcgcacaggt gcgatacgga tctttaatga
    10141 taatttcggt tagcaggcga tcgcatgggc aggccaggta gtcctcgatt gacagcgttt
    10201 cgccgttgcc aaaaaccagt tcagcatcca gtgccaacag gacgggaagc agcaccgact
    10261 cttcctggcg ggcggcgatt tcaccaccaa tggtcgactg attacgaaca tggcgtgagt
    10321 aaacaaaacc gagggcttca cgcagcgctg caggaataaa tcgcgcatca cgcagtggct
    10381 gcaagcgaga cattgcgcca atccgcagtg caccgttatc ccagtcaacc cagtccagtt
    10441 ccagatcctg taaggaaatg gcaatctttt tatcggtacg ggttggtgta gcattgagtt
    10501 tgctgccccc ggcgaaccag acggcttcat cctggtagcg gcgcttcagt tccagcgcct
    10561 gttcgacaga gtcgggcctg aaaaattgtt caatcatctt ctttccttat ttaaccctct
    10621 tccctgcggg aagagggcat ctgtcattta tgccagcgca tccatccgac gccacatact
    10681 ggcagcggct tttctggctt gtgcataaat ggaatcgcaa tcgaagttaa actgacggtc
    10741 ttcatagacc atcacaccat tgaccatcac gctgtgaacg ctgcctgagc ccataccgaa
    10801 agcgatatgc ccggcgatat tgtctgccag cagcggcgtc ggtgagttgt aatcgcaaat
    10861 ggttaaatca gctttgtaac cggcttccag aagtccaaat ttcgcgccaa agttgcggct
    10921 catcagttcg ttaccgttag tcagggcttt ggcaaaactg tcaggccaca gcggaccacc
    10981 cgcatcgcga tgtttaaaga aggcaaattt catctcttca aacatgtccg aaccaatgcc
    11041 gtccgttccc aacgccagat tgcggatgtc gctaaggtga tggttgtagc cgacatggtt
    11101 gttcatgttt gaacgggcgt tatgcaccag gaacgcatcg cgctgattga gtagggcgat
    11161 gtcatctttc gacaagtaca gcccatgagc gaccagcgtt ttgctatcga tgagatcgaa
    11221 ttgcgctagt cgtgccagca ggtctttgcc gtaccagtgg tgactgtagg aaacgtcgta
    11281 aaggtcttcc gcagcgtgaa tatgcaaacc acggcctgtg gctttcacgg cttcacgtag
    11341 catctccaga ccggcatctg gcacggtaaa cggcgcgtga gcaccgatat gtgcttccac
    11401 cagatacggc tcggttgctg ctttcttcgc ctcatcaatc tgacgggcga agcggatgtt
    11461 ttcttctaca ccttcctgca actctttgat gccgttgtta cggtcagtag tttcaaaaca
    11521 ggtcatcgcg cgcaggccaa cttttaaaaa tgcgtcgcgc aatgtggaga gcgacccgcc
    11581 gatatacgcc ggagaggcat ggtgatcgat aaccgatgta catccgctct taatcgcttc
    11641 cagggaacaa atcagcccgc tgtaatagag cgactcttca tcaagggcgc gatcgagccg
    11701 ccaccagaga tttttcagcg ttgagatgaa atccgggcag ggggcgatgt ttgccataat
    11761 tccgcgggaa agccccgagt aaaaatggtt gtgcgagcag acaattcccg gcatcacaat
    11821 ccgaccatgc atctctttgt agctggcatc ggggtagcgt tgcgtcaggg catcgccgat
    11881 agcgacaatc acatcatttt cgatggcgat atcaacgcct tcctgcactt tcgccgggtg
    11941 taactgtact gcggtgacat tcttcagaat caacatgatt acacctccac gcggcccagc
    12001 agataatgat gatgctggtg gacatggctg atgatgcggc acatatcgtt cagctccggt
    12061 ggtacgttgt cgaactggcc ttcgctgtcg atgttcagca cccagctttg gttattcaga
    12121 cgtacgcgta cccgacaatc ttccaccagg aagcctgggt tgctgctgtt atcaaagtct
    12181 tgcgacaggc tgaagacggt gattttgtct ttgtacggtt taccgttcca cggacagaac
    12241 tgagcgcagt tgccgcattc gttacagtaa gcgtcgaggt gcagcgtctg gaaacggttc
    12301 tggaagcctg ggaccgcaat ggagacgttg gcgcggttcg ggcagacatc cacacacttg
    12361 ctgcaaacgt agttacattc gaggcagcga gcggcttcct gcgcgacaaa cgcatcacga
    12421 tcgtcactgt tcaccagcgt gatagagata tcgcctttac gttgatagat ttccgccgga
    12481 ttgacgttgt tccagtattt attgttctgg tgggaacgga tattttcccg actaaggatg
    12541 gcatcggtcg cccgacgcgc ggttccgaca gcagcgacaa tggaggatgg tccgcgctgt
    12601 acgtcgccga tcataaagac gtcagtcaga cgagtttcgc cattatggtc gacgtctggc
    12661 cagccgtttt tgtccagcgg tacgcccatc gcattcaggg cttcagtatc ctgctgttca
    12721 ccgatggcgg tgatcaggct gtctacatgc agtgttactg tttcgttggt ttcaaccgga
    12781 cggcgacgac ctttctcatc gggttcgcca agcgacataa cgcgcaaggt taaggtgcca
    12841 tcagcatcga aacattccgg attattcagg aaacggaact ccacgccgtc gtgcaacgct
    12901 tcttcatact cttcgcgcca tgcgggcatc tcttgcagtg aacgacggta aacgatcgtt
    12961 gctttttcta cgcctggaac gcgtaacgcc gcacgagcgc agtccattgc ggtgttaccc
    13021 gccccgacaa cgaccacatg tttgcccagc ttgagcgctg tacccttgtt gtattcacgc
    13081 aggaagggga gtgatttcca gacattttgg ttgtcgcccg ccagtttcac accgctattt
    13141 ttatcagtgc cggtggcaat cagaacatag tggaagccct gattttttaa ctgttcaacg
    13201 gtcaaatcgg gtgagcagcc atactcaaat ttcacgccgt gagcggcaac aaaatcgata
    13261 tcgtgctgaa ttaactctgc aggaatacgg aactgaggaa tgatattttt caccacgccg
    13321 ccagcattgg cttcgcgttc aaacagcgta accggatggc ccgctctggc aaggaagtaa
    13381 cctgctgcca gacccgccgg acctgcacca atcacggcaa ccggatggcg tgaaccagaa
    13441 ccggcgggtt tgtgccagcg ttgcttatat tcatcccaac ctttttccag cgcgactttt
    13501 ttcagttcgc ggatattcag cgcactgtcg taatccaggc gggtacagtt gtattggcac
    13561 tggtgatcgc aaatatgacc ggtaatggcg ggcagagcgt tgtgttggta gatgagttcc
    13621 agcgcgtcgg catagcggtg ttcgccaagc agacggatgt attccggaat atcttgcttg
    13681 atagcgcagg cagtaacaca gggggcaacg tagcaatcgg tcagcggcag gtcttctgcc
    13741 acttcaatac gctcttctgg cttccagtgt ttctgggtgt attccatggt taacgcatct
    13801 gctgccagtc tgttcagtcg ttcgacgtca acatggtcaa gtccccaggc gtcggagcct
    13861 tccagctcgc gcatgcaggc acttaagcgc aaatagccgc caggtttcag caggtcggtt
    13921 gccatagtaa tagggcgaat acctgtatca aaaatatcgc ggatagtcag ctgactggca
    13981 ccaccggaat aagaaatggg cagtttgccg tcaaaggcgc gagagagaac tgctgcaaca
    14041 ttgatggaga gcgggaacag cgcacggcct gacatataca tctcttcacc aggcagtgcg
    14101 cctttattgt tgatggtgcc gagagtgtta gtcagtttta cgccaaagcc gagtgatttt
    14161 tcttttgcca gtgccatcag gcgttccagc atttccagtg cttgcgtcag cttgaggtcg
    14221 tgatcaaatg actcttcttt taagcctatg taaccgaaac cgcagacatc gaggatctca
    14281 cgaacacgcg cgtatcccag taaggtcggg ttaagtttca caaaggtgtt gagccctttt
    14341 tcttccagca tgtagcggca aatggcttcg atttcatgcg gaggacagcc gtgcatggtg
    14401 gagagggtga cgccctgcac catactggtg gggatgcgag cgggtaaggc ttgcaagctt
    14461 tcgcgttttt cctgcaatcc gtggcgggct aaaaatgcgt catcctggag taatttattc
    14521 agcgtatcgc gatattgcgc gaatttcgga tggtcagatg cgtccatcat attgtcgatg
    14581 aactgttgca ttgacggttg cttaatacct tcgaggttgt aaccgacgct catattaaag
    14641 atgaacgatt taccggaatc agaaggctgg aacatcgctt cgagaaggtg cagggcaaac
    14701 caggctttga ggtattcatc ccaggcttta agcagggtaa attcggtaga ccattcggtg
    14761 ttaaagcact cgtcttcggc atcgatacag ggcttttcca gctccaggcg gtcaagaatt
    14821 tggacggttt ttagttcgat gaagcgtccg ccagtcagcc aggaagtgac aatattttgc
    14881 gcgagctgcg tgtgcgggcc agcggcaggg ccgacgggag tggcacaggt ttcaccgaag
    14941 acgctaacag ttttaccttt tacaggtgag taaaactgtt gctcgggaat accaaagatt
    15001 gagcgttgtt gttggtattc atcaaatatg cgcgtcaaaa gttcctcaaa cggaatggga
    15061 cgcataatat cccccataac cctctcctta gcttatacag atatcattga gtgaccggtg
    15121 ttgagaaatc cggtggttta gggggataga gcaacaatcg caccactctt gccaggtttg
    15181 gcgggaaata taaaaaaatg tgaggcggct cgaaacttaa aggctttttg tgaatgaact
    15241 cacaggtgcc aaatgagaat taaaatcact cctgaaaatt attattgtca gcccatttat
    15301 cactttttct cttttct
//
LOCUS       DAZFRN010000121        15019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFRN010000121 DAZFRN010000000
VERSION     DAZFRN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.123"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            116..358
                     /gene="yjbE"
                     /locus_tag="ACHKUB_003609"
     CDS             116..358
                     /gene="yjbE"
                     /locus_tag="ACHKUB_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO6656572.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            472..1110
                     /gene="yjbF"
                     /locus_tag="ACHKUB_003610"
     CDS             472..1110
                     /gene="yjbF"
                     /locus_tag="ACHKUB_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO6656573.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1107..1844
                     /gene="yjbG"
                     /locus_tag="ACHKUB_003611"
     CDS             1107..1844
                     /gene="yjbG"
                     /locus_tag="ACHKUB_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6656574.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1844..3940
                     /gene="yjbH"
                     /locus_tag="ACHKUB_003612"
     CDS             1844..3940
                     /gene="yjbH"
                     /locus_tag="ACHKUB_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6656575.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3987..4265)
                     /locus_tag="ACHKUB_003613"
     CDS             complement(3987..4265)
                     /locus_tag="ACHKUB_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656576.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4281..4409
                     /locus_tag="ACHKUB_003614"
     CDS             4281..4409
                     /locus_tag="ACHKUB_003614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656577.1"
                     /translation="MVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYPAYG"
     gene            4535..4945
                     /gene="psiE"
                     /locus_tag="ACHKUB_003615"
     CDS             4535..4945
                     /gene="psiE"
                     /locus_tag="ACHKUB_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO6656578.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4989..6464)
                     /gene="xylE"
                     /locus_tag="ACHKUB_003616"
     CDS             complement(4989..6464)
                     /gene="xylE"
                     /locus_tag="ACHKUB_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO6656579.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSILQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6836..7726)
                     /gene="malG"
                     /locus_tag="ACHKUB_003617"
     CDS             complement(6836..7726)
                     /gene="malG"
                     /locus_tag="ACHKUB_003617"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO6656580.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7741..9285)
                     /gene="malF"
                     /locus_tag="ACHKUB_003618"
     CDS             complement(7741..9285)
                     /gene="malF"
                     /locus_tag="ACHKUB_003618"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO6656581.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9439..10629)
                     /gene="malE"
                     /locus_tag="ACHKUB_003619"
     CDS             complement(9439..10629)
                     /gene="malE"
                     /locus_tag="ACHKUB_003619"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO6656582.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10994..12109
                     /gene="malK"
                     /locus_tag="ACHKUB_003620"
     CDS             10994..12109
                     /gene="malK"
                     /locus_tag="ACHKUB_003620"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO6656583.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12181..13521
                     /gene="lamB"
                     /locus_tag="ACHKUB_003621"
     CDS             12181..13521
                     /gene="lamB"
                     /locus_tag="ACHKUB_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO6656584.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFYNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13764..14684
                     /gene="malM"
                     /locus_tag="ACHKUB_003622"
     CDS             13764..14684
                     /gene="malM"
                     /locus_tag="ACHKUB_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO6656585.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14913..>15019
                     /locus_tag="ACHKUB_003623"
     CDS             14913..>15019
                     /locus_tag="ACHKUB_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656586.1"
                     /translation="MISHILNSGGNNSLMKHDVVQGNNIVDLDLLRNFNG"
BASE COUNT         3710 a         3866 c         3958 g         3485 t
ORIGIN      
        1 aaaaccccat ctctaattcc ccgcgccgta attcgcatgc tttagttgtg tatactcgat
       61 cccgcccgaa atgtttttgg gtaaatctcc attcattcaa tgaagggaaa ttgttatgaa
      121 aaaagttctg tatggcattt ttgccatatc tgcgcttgcg gcgacttctg cgtgggctgc
      181 acctgtacag gtgggcgaag cggcagggtc ggcagcaacg tcggtttcgg cggggagttc
      241 ctccgcgacc agcgtcagca ccgtaagctc ggcggtgggt gtcgcgctcg cggcaaccgg
      301 tggcggtgat ggttctaata ccgggaccac cacaaccacg accaccagta cccagtaata
      361 aagtatgtat ccccaaaata atttgagtca ttgcatctgt ggcttgaagt atgaagggat
      421 ttaccataac cacactccgg tgtggttatt ctgcccctct ggagaagagt cgtgaagcga
      481 cctgcactca ttcttatctg cctgctatta caggcctgtt cagccacgac taaagagctg
      541 ggcaattcac tgtgggacag tctgttcggc acgccaggcg tacagctgac ggacgatgat
      601 attcaaaata tgccctacgc cagccagtac atgcagctta atggcgggcc gcaattgttt
      661 gtggtgctgg ccttcgctga agacggacaa caaaaatggg tcactcagga tcaagccact
      721 cttgtcatac aacatggtcg tctggtgaag actttgcttg gcggcgacaa cctgattgaa
      781 gtgaataacc ttgccgccga cccgctgatt aaacccgcac aaattgttga tggtgcaaca
      841 tggacgcgca cgatgggctg gaccgagtac cagcaggtac gctacgccac cgcacgctca
      901 gtcttcaaat gggatggcac cgataccgtc aaggtcggca gcgatgaaac cccggttcgc
      961 gtgctggacg aagaagtctc caccgaccag gcgcgctggc ataaccgcta ctggatcgac
     1021 agcgaagggc aaattcgcca gtcggaacag tatctcggcg cggattattt cccggtgaaa
     1081 accactctca tcaaggcggc aaaacaatga ttaaacaaac tattgtcgca ttgattttga
     1141 gtgtgggggc gtcatcggtc ttcgcggcgg gaaccgtcaa ggtattcagc aatggcagca
     1201 gtgaggccaa aacgctgacg ggcgcagagt atttaatcga tctggtaggc cagccgcggc
     1261 tggcaaacag ttggtggcct ggtgcggtga ttagcgaaga gctggcaacg gcggcagcat
     1321 tgcggcagca gcaggcgttg ctgacacggc tggcagaact ggctgcagat tccagcgccg
     1381 acgatgccgc cgcgattaac gccttacgcc agcaaattca ggcgttgaaa gtgacgggca
     1441 ggcaaaaaat caatcttgat cccgatatcg tccgcgttgc cgaacgcggt aacccaccgt
     1501 tgcagggcaa ctacacgctg tgggtcgggc caccaccgtc cacggtcacg ctgttcgggc
     1561 ttatcagccg tcctggcaag cagccattca ctccaggtcg cgacgtggcg agctatctct
     1621 ccgggcagaa cctgcttagc ggtgcggatc gcagctacgc gtgggtggtt tacccggacg
     1681 gacgcacgca aaaagcgccg gtggcttact ggaacaagcg tcacgtagag ccgatgcccg
     1741 gcagcattat ttatgttggc ctcgcggact ccgtctggag tgagacgcct gatgccctta
     1801 acgccgacat tcttcagact ctgacgcagc ggatacccca ataatgaaaa aaagacatct
     1861 gcttagctta ctggcgctgg gcattagcac agcttgctac ggcgaaacat atcctgcgcc
     1921 cattggtccg tcgcagtcgg atttcggtgg cgtaggatta ttacaaactc ccaccgcgcg
     1981 tatggcacgg gaaggggagt tgagtctgaa ctatcgcgat aacgatcagt accgttatta
     2041 ctcagcttca gtgcaactct tcccgtggct ggaaacaacg ctgcgctaca ccgacgtgcg
     2101 cacccggcag tacagcagcg tcgaagcgtt ctctggcgat caaacgtata aagataaagc
     2161 cttcgatctc aaactgcgtt tgtgggaaga gagttactgg ctgccgcaag tggcggttgg
     2221 tgcgcgggat atcggcggta cggggctgtt tgatgcggaa tatcttgttg ccagcaaagc
     2281 ctgggggccg ttcgatttta cgctcggcct gggctgggga tatctgggca ccagcggtaa
     2341 tgtaaaaaat ccgctctgtt cagccagtga taaatattgc tatcgcgata acagctacaa
     2401 acaggcggga tctatcgacg gtagccagat gttccacggt cctgcctcac tgtttggcgg
     2461 cgtggaatac cagacgccct ggcaaccgct gcgcctgaaa ctggagtatg aaggcaataa
     2521 ttatcagcag gattttgccg ggaagctgga gcaaaaaagt aagtttaacg tcggtgcgat
     2581 ttatcgcgtt accgattggg ctgacgttaa ccttagctat gaacgtggca acacctttat
     2641 gtttggcgtc acgctgcgca ccaactttaa cgatctgcgc ccgtcttaca acgataacgc
     2701 ccgcccgcaa tatcaaccgc agccgcagga tgccattttg cagcattcgg tggtggcgaa
     2761 tcagttaacg ctgttgaaat acaatgccgg acttgccgat ccgcagatcc aggcgaaagg
     2821 cgatacgctg tatgtcaccg gcgagcaggt gaaatatcgt gattcgcgcg aagggatcat
     2881 ccgtgctaat cggatcgtga tgaacgatct gccggatggg atcaaaacga tccgcattac
     2941 ggaaaatcgt cttaacatgc cgcaggtgac gacggaaacc gatgtcgcca gcctgaaaaa
     3001 tcatctcgcc ggagagccgt tgggccacga aacgaagctg gcgcaaaaac gcgtcgagcc
     3061 agtggttccg cagtccaccg agcagggctg gtatatcgac aaatcacgct ttgatttcca
     3121 catcgatccg gtgctgaacc agtcggtcgg tggcccggaa aacttttaca tgtatcagct
     3181 gggcgtgatg ggaacggcag atttgtggct gacggaccat ctgctgacca ccggcagcct
     3241 gtttgcaaat cttgccaaca actacgacaa gtttaactac accaatccgc cgcaggactc
     3301 gcacttaccg cgcgttcgta cccatgtgcg cgagtatgtg cagaacgatg tctatgtgaa
     3361 taacctgcaa gccaactact tccagcatct gggcaacggc ttctacggtc aggtctatgg
     3421 aggttatctc gaaaccatgt ttggcggtgc gggggccgaa gtgttgtatc gtccgctgga
     3481 cagtaactgg gcgtttggtc tggatgccaa ctacgttaaa cagcgcgact ggcgtagtgc
     3541 aaaagatatg atgaaattca ccgactacag cgtgaaaacc ggacatctga ccgcctactg
     3601 gacgccatct ttcgcccagg acgtgttggt gaaagccagc gtcgggcagt atctggcagg
     3661 ggataaaggc ggcacgctgg agatcgccaa acgctttgat agcggcgtgg tggttggcgg
     3721 ctatgccacg atcaccaacg tttcgaaaga ggagtacggc gaaggggact tcaccaaagg
     3781 cgtgtatgtc tcggtaccgt tggatctctt ctcgtctggc ccgacacgca gccgtgcggc
     3841 gattggctgg acgccgctga cgcgtgacgg tggtcagcaa cttgggcgta agttccagtt
     3901 gtatgacatg accagcgacc gtagcgtcaa tttccgctaa gtcatgggaa aggtgccagt
     3961 tttcgcaatc actggcaccc tcattcttaa tggcctgttg agccatcagg aacgcagttc
     4021 acccccggtt tgcgatctgg tgtgtaaagg cgaaaacatt ctgtcttcct gtccgtcgat
     4081 atcggttgca gatctggttg cctgagcgac tgggccatca ttgggctgga gacattgctt
     4141 tgcggtaggt cggctttatc agcagtaccg agcggaccag cataagcagg aagaacagag
     4201 actgataaca tcaaagcagc aaaataaggc ttcattttta ccacctttat caggttacgt
     4261 ttcatttgtt ccagaggaac atggtcgatt tttcgcgcat tgctggtggc tgggaatcac
     4321 ctgaatgggt gatttttgaa ttaccggctt tggtgcggtt tgtcttgccg gatgcgccgc
     4381 caggcgcggc ttatccggcc tacgggtagg tatatccggc tttggtggag gcgcgctcca
     4441 aatccaggtt gaacaaaaca tacacaaaaa atatagatct ccgtcacatt tttgcgttat
     4501 acaggaagct cgccactgtg aaggaggtac tgctatgacg tcactctctc gtccgcgcgt
     4561 ggagtttatc tccaccattt tgcagaccgt actcaatctt ggcctgttgt gcctcggcct
     4621 gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg gctgatgtgc tattcgcgcc
     4681 agaacaaacc agcaaatatg agctggtaga aggactggtg gtttactttc tctatttcga
     4741 atttatcgcg ctgattgtga agtactttca gtccggtttt cacttcccgc tacgttactt
     4801 tgtctatatt gggatcaccg ccattgtgcg cttgatcatc gtcgatcata aatcgccact
     4861 ggatgtgttg atctactcgg ctgcgatcct gttactggtg atcaccctgt ggctgtgtaa
     4921 ttcgaaacgg ctgaagcggg agtaaaaaga cagcacgccg aactggcgcg gcgtgctgga
     4981 caggaagatt acagcgtagc agtttgttgt gttttcttcg tttccggttc ccagagggct
     5041 tccagctcct caagggtttt acctttggtt tccgggacaa atttccacat aaatagtgct
     5101 gccagaacgc ccatacaccc gtaaatccag taggagaaac cgttgtggaa atgggccacc
     5161 agccaggagt ttttgtccat catcgggaag gtccaggaga cgaagtagtt cgccagccac
     5221 tgggccgcca ccgcgattgc cagcgcttta ccacgaatag cattcgggaa gatttccgac
     5281 agcagtaccc agcataccgg accccaggac atggcaaagg cggcaacata gaacagcatc
     5341 gacagaagcg ccacaatacc cggtgcctga gtgtaaaacg cggtaccgag gctaaacata
     5401 ccgattgcca ttccgagtgc gccgataatt tgcagtggct tacgaccaaa tttatccacc
     5461 gtcataattg ccagaacggt gaaggtgagg ttgataactc cgacaataat ggtctgcaac
     5521 agcgcgatat ccgtgctggc ccccagcgtt ttgaacactt ccggcgcgta gtacagcacc
     5581 acattgatgc cgacaaattg ctggaggatg gagagcataa cgccgatgac aatcacgcct
     5641 acgccaaaca tcagcagacg accaccggtt ttgcggccat gatccagaga gtgtttaatt
     5701 tcctgtactg cctgagttgc aagggtgttg cccataattt tgcgcaggat accttccgcc
     5761 tgttcttgct tgccgcgtga catcagccag cgaggacttt ctggcacggt atacagcagc
     5821 attaagaaca gcagtgcagg gatacattcc gaggcaaaca tataacgcca gccgtcagta
     5881 ttcagccagc tggcgtcgcc ggaacgggca ataaaatagt ttacgcagta aactaaaagc
     5941 tgcccgaaaa taatcgcaaa ctggttaaaa gagaccagtt tcccgcgaat atgagcagga
     6001 gcaagttccg caatatacat tggtgaaagc attgaggcta aaccaacgcc aataccgcca
     6061 ataatgcgat aaataacaaa ttccgggaca taacctgcca gataaacagg cactgtgttg
     6121 tccgggttta tagaggtaaa accaagttct ggccaggcag aacctacacc agaaataaaa
     6181 aacaggacag cagcaatctt aagtgaatca cgacgaccga agcggctact gcaataacca
     6241 ccgagggcac cgccgatgat gcaaccaatc agagcgctgg ccacgcaaaa ccctaacagg
     6301 gagttggcag cggattcact taagttttgt ggagcaacaa agacggtatt gagtgactca
     6361 acagtaccgg aaataacggc ggtgtcgtag ccaaataata aaccacctaa tgtagcgact
     6421 aaggtaatcg aaaatatata actggaatta tactgggtat tcattcagac ctgccttaga
     6481 ccattctgat cttttatcag tgcattgatg ttggaattgt caggcagtta aggcaattat
     6541 cattttttac aatgcgttta cgtaatgtct tttctgtgat cttaattgtg ataattatca
     6601 aaaattgatg aaaacaatac tattgccgtg actcagagca cgaaagagaa ttatcgtaag
     6661 tgggaaaaca aataacgtaa aaataataag ttctatgatg gaaatattaa ccggcgaacg
     6721 attcagattg cagacgaaag aaaaaaaggc gctccgtgga gcgccgaata acagtcacaa
     6781 gttgggataa cttaagttga gggtgcagcg gcataacatt ggcagaacaa catctttaac
     6841 ctttcacacc acctgccgtc aggccgttga ccagccagcg ttgagccagc aagaagacga
     6901 tggtgatcgg taatgcagac atcacggcag cggcggcaaa gtcaccccac aggtagtttt
     6961 gcgggttgag gtattgctgc atccccacgg ccagggtgta gctgtttacg tcacgcagta
     7021 acagcgacgc gaccggaact tcagtaatgg cagcgataaa cgacaggatg aacaccaccg
     7081 ccagaatcgg tactgacagc ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac
     7141 catccagcgc cgccgcttct tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg
     7201 tccagacgtg cagcgcaatc ccacccagat acgcgaaaat tacgccgccg tgagtattca
     7261 ggccaatgaa tggaatgtac tcacccagac gatcaaacaa cgcatacaac gcgaccagtg
     7321 aaagtactgc cgggaacatc tggaaaatca gcattccttt cagcagcgtc gctttgcctg
     7381 gaaagcgcat acgggcgaaa gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg
     7441 cggaaatccc ggcgaccttt accgagttcc acagccacag cagtaccggg aatggcggtg
     7501 gcgtaatgcg accatcagcc tgttccacgc taaaacctaa tgccagtttc cagtgatccc
     7561 aggagatttg ctccgggatc aggctaccgg tcgcaaagtt cccctggcgc agtgagatgg
     7621 cgacgaccat cagcagcggg aacataatcg ccgcgataaa aagtagcagt agcaggtgag
     7681 taataaacaa acgtgctttt tgcgatttcg gttggaccat tgccattttt gttatctccc
     7741 ttaatcaaac ttcattcgcg tggctttcag gttcactatc gccagcgcac ccaccagcag
     7801 gaagatcagc gtggctattg ctgctgccag accgaagtcc tgacccccgc cgccttcaaa
     7861 agcgatgcgg taggtgtagt taacaagcag gtcggtataa ccggctggcg tggtcgtgcc
     7921 aagacgatcc gggccgccgt tggttaacag ttgaatcagc acgaagttgt taaagttaaa
     7981 ggcgaagctg gcgatcatca gcggcgtcag cggtttaatc agcagcggca gcgtaatctt
     8041 aaagaagttc tggaagggac ctgcaccatc cattgctgag gcttcataca aatcgtccgg
     8101 aatcgctttc agcaagccca tgcagaggat catcatgtac ggataaccca gccaggtatt
     8161 gacgataatc agcatcgttc gggcggtggt cggatcgctg aaccaggcgg gcttcacgcc
     8221 aaacagcgcg ctcaacatca tgttgatctc accgaagctc tggttaaaca accctttgaa
     8281 aatcaagatt gaaatgaatg atggcaccgc gtagggcaga atcagcagga cgcgatagac
     8341 cgctttgccg cgcaacgctt cccactgcac cagacacgcc agaaccatgc cgaccgccac
     8401 cgttaaaaag acagtgatca gcgagaacac cacggtccag acgaaaatgg cgaggaacgg
     8461 tttctgaatg ccttcgtcgg tgaagacgcg ggtaaagttt ttccagccag tagtcacggt
     8521 gtaaccgggg cttagctttt catcgcccca gttgccgtcg gcggtaatgg actggtaaaa
     8581 gccaatttgg ttattcggac gatatttcac gccgctctga ttattcgtca acgtgccgtc
     8641 accgtcgagt gtgtagagcg gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac
     8701 tttgttgcca tccggcagaa tggcggtaat gtcactcagc gcctgacgat tctgggtaat
     8761 cacgcgcaga tttgcgcgtt cgccttcggg ctgggcggtc gtttctttca gttgcagttt
     8821 ttgctcgccg ccaaatttaa aagcgtcgga gaggtaattt ttgccggttt cgccgtcgct
     8881 gagggctagt tgccactcat cgcccgccgg gtaaagacca aaattatagg ttttgcctgc
     8941 ttgccaggag cgatctaaca acacttcctg cgcacgttca aaagtcagct ggttagtgct
     9001 gctgtagttg gtgaaggcaa tggcgatggt gcagaccaga gggaagagga cgaataatcc
     9061 cattccggcc attcccgggt aaacatagcg ccaggcgtag gctttacgat tggcgaaaat
     9121 atacagcccc gctgaactca atatcagcgt ggtaatggca aacaggtatt ccccttgtgc
     9181 gtacattaaa acaacaaggt aacccaccag caggccgagc agacctagca ctgaccattt
     9241 cagcgcgtcg ctttgccacc aatgtttctt tttaatgaca tccatggggt tcttcctcat
     9301 tccaggacgg ataaggcttt cacgccttat ccgacaacaa ctgcctgatg cgacactgat
     9361 gcgtcttatc aggcctacat acgtttcggt tttgtaggcc ggacaaggcg ttcacgccgc
     9421 atccggcatt tcacagcatt acttggtgat acgagtctgc gcgtctttca gggcttcatc
     9481 gacagtctga cgaccgctgg cggcgttgat caccgctgta cgcacggcat accagaaagc
     9541 ggacatctgc gggatgttcg gcatgatttc acctttctgg gcgttttcca tggtggcggc
     9601 aatacgtgga tctttcgcca actcttcctc gtaagacttc agcgctacgg cacccagcgg
     9661 tttgtcttta ttaaccgctt ccagaccttc gtcagtcagc agatagtttt cgaggaactc
     9721 tttcgccagc tctttgttcg gactggcggc gttaataccg gcgctcagca cgccaacgaa
     9781 cggtttagat ggttgaccct tgaaggtcgg cagtaccgtt acaccataat tcactttgct
     9841 ggtgtcgatg ttggaccatg cccacgggcc gttgatggtc atcgctgttt cgcctttatt
     9901 aaaggcagct tctgcgatgg agtaatcggt gtctgcattc atgtgtttgt ttttaataag
     9961 gtcaaccagg aaggtcagac ccgctttcgc gccagcgtta tccacgccca cgtctttaat
    10021 atcgtacttg ccgttttcat acttgaacgc ataacccccg tcagcagcaa tcagcggcca
    10081 ggtgaagtac ggttcttgca ggttgaacat cagcgcgctc ttacctttcg ctttcagttc
    10141 tttatccagc gccgggatct cttcccaggt ttttggtggg ttcggcagca gatctttgtt
    10201 ataaatcagc gataacgctt caacagcgat cgggtaagca atcagcttgc cgttgtaacg
    10261 tacggcatcc caggtaaacg gatacagctt gtcctggaac gctttgtccg gggtgatttc
    10321 agccaacagg ccagattgag cgtagccacc aaaacggtcg tgcgcccaga agataatgtc
    10381 agggccatcg ccagttgccg caacctgagg gaatttctct tccagtttat ccggatgctc
    10441 aacggtgact ttaattccgg tatctttctc gaatttctta ccgacttcag cgagaccgtt
    10501 atagccttta tcgccgttaa tccagattac cagtttacct tcttcgattt tggcgagagc
    10561 cgaggcggaa aacatcatcg tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt
    10621 tattttcata atctatggtc cttgttggtg aagtgctcgt gaaaacacct aaacggactc
    10681 tagtttcttt atacggcaac ctctttccat cctccttgcc cctacgcccc accgttgctt
    10741 tgtgtgatct ctgttacaga attggcggta atgtggagat gcgcacataa aatcgccacg
    10801 atttttgcaa gcaacatcac gaaattcctt acatgacctc ggtttagttc acagaagccg
    10861 tgttctcatc ctcccgcctc ctcccccata aaaaagccag ggggtggagg atttaagcca
    10921 tctcctgatg acgcatagtc agcccatcat gaatgttgct gtcgatgaca ggttgttaca
    10981 aagggagaag ggcatggcga gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt
    11041 cgtggtatcg aaagatatca atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg
    11101 accgtctggc tgcggtaaat cgactttact gcgcatgatt gccgggcttg agacgatcac
    11161 cagcggcgac ctgttcatcg gtgagaaacg gatgaatgac actccgccag cagaacgcgg
    11221 cgttggtatg gtgtttcagt cttacgcgct ctatccccac ctgtcagtag cagaaaacat
    11281 gtcatttggc ctgaaactgg ctggcgcaaa aaaagaggtg attaaccaac gcgttaacca
    11341 ggtggcggaa gtgctgcaac tggcgcattt gctggatcgc aaaccgaaag cgctctctgg
    11401 cggtcagcgt cagcgtgtgg cgattggccg tacgctggtg gccgagccaa gcgtattttt
    11461 gctcgatgaa ccgctctcca acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat
    11521 ctcccgtctg cataaacgcc tgggccgcac aatgatttac gtcacccacg atcaggtcga
    11581 agcgatgacg ctggccgaca aaatcgtggt gctggacgcc ggtcgcgtgg cgcaggttgg
    11641 gaaaccgctg gagctgtacc actatccggc agaccgtttt gtcgccggat ttatcggttc
    11701 gccaaagatg aacttcctgc cggtaaaagt gaccgccacc gcaatcgatc aagtgcaggt
    11761 ggagctgccg atgccaaatc gtcagcaagt ctggctgcca gttgaaagcc gtgatgtcca
    11821 ggttggagcc aatatgtcgc tgggtattcg cccggaacat ctactgccga gtgatatcgc
    11881 tgacgtcatc cttgagggtg aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat
    11941 ccatatccag atcccttcca ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt
    12001 ggtagaagaa ggtgccacat tcgctatcgg cctgccgcca gagcgttgcc atctgttccg
    12061 tgaggatggc actgcatgtc gtcgactgca taaggagccg ggcgtttaag caccccacaa
    12121 aacacacaaa gcctgtcaca ggtgatgtga aaaaagaaaa gcaatgactc aggagataga
    12181 atgatgatta ctctgcgcaa acttcctctg gcggttgccg tcgcagcggg cgtaatgtct
    12241 gctcaggcaa tggctgttga tttccacggc tatgcacgtt ccggtattgg ctggacaggt
    12301 agcggcggtg aacaacagtg tttccagact accggtgctc aaagtaaata ccgtcttggc
    12361 aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat
    12421 aagagcttct atttcgacac taacgtggcc tattccgtcg cgcaacagaa tgactgggaa
    12481 gctaccgatc cggccttccg tgaagcaaac gtgcagggta aaaacctgat cgaatggctg
    12541 ccaggttcca ccatctgggc aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc
    12601 gacttctact actgggatat ttctggtcct ggtgccggtc tggaaaacat cgatgttggc
    12661 ttcggtaaac tctctctggc agcaacccgc tcctctgaag ctggtggttc ttcctctttt
    12721 gccagcaaca atatttatga ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt
    12781 ttagcgcaga tggaaatcaa cccgggcggc acattagaac tgggtgtcga ctacggtcgt
    12841 gccaacctgc gtgataacta tcgtctggtt gatggcgcat cgaaagacgg ctggttattc
    12901 actgctgaac atactcagag tgtcctgaag ggctttaaca agtttgttgt tcagtacgct
    12961 actgactcga tgacctcgca gggtaaaggt ctgtcgcagg gttctggcgt cgcgttttat
    13021 aacgaaaaat ttgcctacaa tatcaacaac aacggtcaca tgctgcgtat cctcgaccac
    13081 ggtgcgatct ccatgggcga caactgggac atgatgtacg tgggtatgta ccaggatatc
    13141 aactgggata acgacaacgg caccaagtgg tggaccgttg gtattcgccc gatgtacaag
    13201 tggacgccaa tcatgagcac cgtgatggaa atcggctacg acaacgtcga atcccagcgc
    13261 accggcgaca agaacaatca gtacaaaatt accctcgcac aacaatggca ggctggcgac
    13321 agcatctggt cacgcccggc tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa
    13381 tggggttatg actacaacgg cgatagcaag gttaacccga actacggcaa agccgttcct
    13441 gctgatttca acggcggcag cttcggtcgt ggcgacagcg acgagtggac cttcggtgcc
    13501 cagatggaaa tctggtggta atagcaaaac ctgggccgga taaggcgttt acgccgcatt
    13561 cgacaaccaa cgcctgatgc gacgcttgcg cgtcttatca ggcctacaac ggctgtcaaa
    13621 tgtaggccgg ataaggcgtt tacgccgcat ccggcataaa aacaggttgt cattatctga
    13681 aaggggcgaa agcccctctg attatcgggt ttagcgcgct attgcctggc taccgctgag
    13741 ctccagattt tgaggtgaaa acaatgaaaa tgaataaaag tctcatcgcc ctctgtttat
    13801 cagcagggtt actggcaagc gcgcctggaa tcagccttgc cgatgttaac tacgtaccgc
    13861 aaaacaccag cgacgcgcca gccattccat ctgctgcgct gcaacaactc acctggacac
    13921 cggtcgatca atctaaaacc cagaccaccc aactggcgac cggcggccaa caactgaacg
    13981 ttcccggcat cagtggtccg gttgctgcgt acagcgtccc ggcaaacatt ggcgaactga
    14041 ccctgacgct gaccagcgaa gtgaacaaac aaaccagcgt ttttgcgccg aacgtgctga
    14101 ttcttgatca gaacatgacc ccatcagcct tcttccccag cagttatttc acctaccagg
    14161 aaccaggcgt gatgagtgca gatcggctgg aaggcgttat gcgcctgaca ccggcgttgg
    14221 ggcagcaaaa actttatgtt ctggtcttta ccacggaaaa agatctccag cagacgaccc
    14281 aactgctcga cccggctaaa gcctatgcca agggcgtcgg taactcgatc ccggatatcc
    14341 ccgatccggt tgctcgtcat accaccgatg gcttactgaa actgaaagtg aaaacgaact
    14401 ccagctccag cgtgttggta ggacctttat ttggttcttc cgctccagct ccggttacgg
    14461 taggtaacac ggcggcacca gctgtggctg cacccgctcc ggcaccggtg aagaaaagcg
    14521 agccgatgct caacgacacg gaaagttatt ttaataccgc gatcaaaaac gctgtcgcga
    14581 aaggtgatgt tgataaggcg ttaaaactgc ttgatgaagc tgaacgcctg ggatcgacat
    14641 ctgcccgttc cacctttatc agcagtgtaa aaggcaaggg gtaattacgc cccacagtgc
    14701 tgattttgca acaactggtg cgtctcctgg cgcacctttt tttatgcttc cttcctggga
    14761 tatgagcgat tttttatagt aactcacttc ttcttcacta agaatatcca ttatctcaat
    14821 gccttatcag agattctttt cctttcgccg gtagtgtctg gacattcagg ctacttttcc
    14881 aggttatttt atttctgtta tgcagaggtt ttatgataag tcatatccta aattctggcg
    14941 gcaataactc tttgatgaaa catgatgtgg tgcaaggaaa taatatagta gatcttgatt
    15001 tactacgtaa tttcaatgg
//
LOCUS       DAZFRN010000122        14952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFRN010000122 DAZFRN010000000
VERSION     DAZFRN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.124"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..525
                     /gene="yehD"
                     /locus_tag="ACHKUB_003624"
     CDS             <1..525
                     /gene="yehD"
                     /locus_tag="ACHKUB_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656587.1"
                     /translation="AAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSV
                     SINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDK
                     AIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            605..1279
                     /gene="yehC"
                     /locus_tag="ACHKUB_003625"
     CDS             605..1279
                     /gene="yehC"
                     /locus_tag="ACHKUB_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6656588.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1295..3775
                     /gene="yehB"
                     /locus_tag="ACHKUB_003626"
     CDS             1295..3775
                     /gene="yehB"
                     /locus_tag="ACHKUB_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6656589.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKHSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3791..4825
                     /gene="yehA"
                     /locus_tag="ACHKUB_003627"
     CDS             3791..4825
                     /gene="yehA"
                     /locus_tag="ACHKUB_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO6656590.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKKEDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(4907..5245)
                     /gene="rcnB"
                     /locus_tag="ACHKUB_003628"
     CDS             complement(4907..5245)
                     /gene="rcnB"
                     /locus_tag="ACHKUB_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO6656591.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5464..6288)
                     /gene="rcnA"
                     /locus_tag="ACHKUB_003629"
     CDS             complement(5464..6288)
                     /gene="rcnA"
                     /locus_tag="ACHKUB_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO6656592.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHDEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6409..6681
                     /gene="rcnR"
                     /locus_tag="ACHKUB_003630"
     CDS             6409..6681
                     /gene="rcnR"
                     /locus_tag="ACHKUB_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO6656593.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6773..6869
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6904..7692
                     /gene="thiM"
                     /locus_tag="ACHKUB_003631"
     CDS             6904..7692
                     /gene="thiM"
                     /locus_tag="ACHKUB_003631"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO6656594.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHEFLSFKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7689..8489
                     /gene="thiD"
                     /locus_tag="ACHKUB_003632"
     CDS             7689..8489
                     /gene="thiD"
                     /locus_tag="ACHKUB_003632"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO6656595.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHYHAWW"
     gene            8554..9372
                     /gene="yegX"
                     /locus_tag="ACHKUB_003633"
     CDS             8554..9372
                     /gene="yegX"
                     /locus_tag="ACHKUB_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO6656596.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            9424..10170
                     /gene="yegW"
                     /locus_tag="ACHKUB_003634"
     CDS             9424..10170
                     /gene="yegW"
                     /locus_tag="ACHKUB_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6656597.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10144..11109)
                     /gene="yegV"
                     /locus_tag="ACHKUB_003635"
     CDS             complement(10144..11109)
                     /gene="yegV"
                     /locus_tag="ACHKUB_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO6656598.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11106..12110)
                     /gene="yegU"
                     /locus_tag="ACHKUB_003636"
     CDS             complement(11106..12110)
                     /gene="yegU"
                     /locus_tag="ACHKUB_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO6656599.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            complement(12107..13384)
                     /gene="yegT"
                     /locus_tag="ACHKUB_003637"
     CDS             complement(12107..13384)
                     /gene="yegT"
                     /locus_tag="ACHKUB_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO6656600.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYAVISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13641..14693
                     /gene="fbaB"
                     /locus_tag="ACHKUB_003638"
     CDS             13641..14693
                     /gene="fbaB"
                     /locus_tag="ACHKUB_003638"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO6656601.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         4014 a         3492 c         3759 g         3687 t
ORIGIN      
        1 gctgccgctg tcttttcttc tttttttatg agcgctggag tatttgctgc agacgttgat
       61 accggaacat taaccattaa aggaaatatt gcagaatctc cgtgtaaatt cgaagcgggt
      121 ggtgattcag taagtattaa tatgccgact gtaccaacca ctgtctttga aggtaaagct
      181 aaatattcta cctatgatga tgcagtcggt gtaaccagca gcatgttaaa aattagctgc
      241 ccgaaagaag ttgctggtgt aaaactctcg ttgattacca acgacaaaat aaccggcaac
      301 gataaggcga tagccagtag caacgatacc gtgggttact atctctattt aggtgataac
      361 agcgatgtcc tggatgtttc tgcacctttt aatattgaga gttataaaac agcggaaggt
      421 caatatgcta ttccgtttaa agcaaaatac ctgaaactaa cagataactc agtgcaatca
      481 ggtgatgtgt tatcttctct ggttatgcgt gtggcgcagg attaataaat aaaattgcaa
      541 gtttctccag gtaataaaca tggccgctat cccatggcgg ccttttaatt taagaggcat
      601 taaaatgaaa ggattattat ctttactcat tttttctatg gtccttcctg cacatgccgg
      661 aattgttatc tacgggacgc gcattattta cccggcagaa aataaagaag ttatggtgca
      721 gttgatgaac cagggaaatc gttcttcgct gctgcaggcg tggattgatg atggcgatac
      781 gtcgttacca ccagaaaaaa ttcaggttcc tttcatgtta acgccaccag ttgcaaaaat
      841 aggggcaaat tccgggcagc aagtaaaaat caaaattatg ccgaataaac tgcccactaa
      901 taaagaaagc attttttatc tgaatgttct ggatattcca ccaaatagtc cagaacaaga
      961 aggtaagaat gcactgaagt ttgcgatgca aaacagaatc aaattgtttt accggccggc
     1021 gggtattgct ccggtaaata aagcgacctt taaaaagtta ctggtaaatc gcagtggcaa
     1081 tggattggtg ataaaaaatg actcagctaa ttgggtgacg atttcggatg tcaaagctaa
     1141 taatgtcaaa gtcaattatg aaactataat gattgccccc ttagaaagtc agagtgttaa
     1201 tgtcaaaagt aataatgcaa ataactggta tctgaccatt attgatgacc acggcaacta
     1261 tattagtgac aaaatttaat tgcaggagct gcctatgttg agaatgaccc cacttgcatc
     1321 agcaatcgta gcgttattgc tcggcattga agcttatgca gctgaagaaa cctttgatac
     1381 ccattttatg ataggtggaa tgaaagacca gcaggttgca aatattcgtc ttgatgataa
     1441 tcaaccctta ccggggcagt atgacattga tatttatgtc aataagcaat ggcgcgggaa
     1501 atatgagatt attgttaaag acaacccgca agaaacatgt ttatcaagag aagttatcaa
     1561 gcggttaggc attaatagcg ataacttcgc cagcggtaag caatgtttaa catttgagca
     1621 acttgttcag ggtgggagct atacctggga tatcggggtt tttcgtctcg atttcagtgt
     1681 cccgcaggcc tgggtggaag aactggaaag tggctatgtt ccaccggaaa actgggagcg
     1741 gggtattaat gcgttttata cctcttatta tgtgagtcag tattacagtg actataaagc
     1801 gtcgggtaat agtaagagta catatgtacg ttttaacagc gggttaaatt tactggggtg
     1861 gcaactgcat tctgatgcca gtttcagtaa aacaaataac aatccagggg tgtggaaaag
     1921 caataccctg tatctggaac gtggatttgc ccaacttctc ggcacgcttc gcgtgggtga
     1981 tatgtacaca tcaagcgata tttttgattc tgttcgcttc agaggtgtgc ggttgtttcg
     2041 tgatatgcag atgttgccta actcgaaaca aaattttacg ccacgggtgc aggggattgc
     2101 tcagagtaac gcgctggtaa ctattgaaca gaatggtttt gtggtttatc agaaagaggt
     2161 tcctcctggc ccgtttgcga ttacagattt gcagttggcc ggtggtggag cagatcttga
     2221 tgtcagcgtg aaagaggcgg acggttcggt aaccacctat ctggtgcctt atgcagcggt
     2281 gccaaatatg ctgcaacccg gcgtgtcgaa atatgatttt gcggcgggtc gtagccatat
     2341 tgaaggggcg agcaaacata gtgattttgt ccaggcgggt tatcagtatg gttttaataa
     2401 cttattgacg ttgtatggtg gctcgatggt tgcgaataat tattacgcgt tcactctggg
     2461 gactggctgg aatacacgca ttggtgccat ttccgtcgat gccacgaagt cgcatagtaa
     2521 acaagacaac ggcgatgtgt ttgacgggca aagttatcaa attgcctaca acaaatttgt
     2581 gagccaaacg tcgacgcgtt ttggtctggc ggcctggcgt tattcgtcgc gtgattaccg
     2641 gacatttaac gatcacgttt gggcaaacaa taaagataat tatcgccgtg atgaaaacga
     2701 tgtctatgac attgccgatt attaccagaa tgattttggt cgcaaaaata gcttttccgc
     2761 caatatgagc cagtcattgc cagaaggttg ggggtctgtg tcattaagta cgttatggcg
     2821 agattactgg gggcgtagcg gcagtagtaa ggattatcag ttgagttatt ccaacaacct
     2881 gcgacggata agctataccc tcgcggcaag ccaggcttat gacgagaatc atcatgaaga
     2941 gaaacgtttt aatattttta tatcgattcc ctttgattgg ggtgatgacg tttcgacgcc
     3001 tcgtcggcaa atatatatgt ctaactcaac gacgtttgat gatcaggggt ttgcctcaaa
     3061 taatacggga ttatcaggaa cagtagggag tcgggatcag ttcaattatg gtgtcaacct
     3121 gagtcatcaa catcagggaa atgaaacgac agctggggcg aatttgacct ggaacgcgcc
     3181 ggtggcaaca gtgaatggca gttatagtca gtcgagtact tatcgacagg ctggagccag
     3241 tgtttcaggg ggcattgtcg cctggtcggg tggcgttaat ctggcgaacc gtctttccga
     3301 aacgtttgct gtgatgaatg cgccaggaat taaagatgct tatgtcaatg ggcaaaaata
     3361 tcgcacaaca aaccgtaatg gagtggtggt atacgacgga atgacacctt atcgggaaaa
     3421 tcacctgatg ctggatgtgt cgcaaagcga tagcgaagca gaattacgtg gcaaccggaa
     3481 aattgccgca ccttatcgcg gcgcggttgt actggttaat tttgataccg atcagcgcaa
     3541 gccctggttt ataaaagcgt taagagcgga tgggcaacca ttaatgtttg gttatgaagt
     3601 caatgatatc catggtcata atattggcgt tgtcggtcag ggaagccagt tatttattcg
     3661 caccaatgaa gtaccgccat cggttaatgt ggcaattgat aagcaacaag gactttcatg
     3721 cacaatcacc ttcggtaaag agattgatga aagtagaaat tatatttgcc agtaatagtc
     3781 aggtggttct atggaaattc gcataatgct atttatatta atgatgatgg ttatgcctgt
     3841 gagctatgcg gcatgttata gtgagttatc tgttcagcac aacttggttg ttcaggggga
     3901 ttttgcactt actcaaacac aaatggcgac atatgagcat aattttaatg attcgtcatg
     3961 cgtaagtaca aatactatca cccctatgag cccgtcggat attattgttg gactttataa
     4021 cgataccata aaattaaatt tacattttga atggaccaat aaaaacaaca tcacgttgtc
     4081 aaataatcag accagtttca ccagtggtta ttcagttacg gtgacacctg cggccagtaa
     4141 tgcaaaagtg aatgtttctg cggggggcgg cggttcagtg atgattaatg gtgttgcgac
     4201 attatccagt gcttcatcat cgacacgcgg gagtgccgca gtacaatttc tactgtgttt
     4261 attaggtggc aagtcatggg atgcatgtgt aaatagctac agaaatgcat tggcacaaaa
     4321 tgcaggtgtc tattccttta atctgacatt gtcatacaac ccgataacca caacctgcaa
     4381 accggatgat ttattaatta ctttagacag tattcccgtt tcacaattac cagccacagg
     4441 taacaaagca acaataaata gtaaaaaaga ggatattatt ctgcgttgta aaaatttatt
     4501 aggtcaacaa aatcaaacat cacggaaaat gcaggtgtat ttatcaagtt ctgacttgtt
     4561 aaccaacagc aacacaatac tgaaaggtgc ggaagataat ggcgtaggat ttattcttga
     4621 aagtaatggt tcgccagtca cacttttaaa tatcactaac agcagtaaag gatatacaaa
     4681 tttaaaggaa gttgcggcga agtcaaaact tacagataca acggtttcaa ttccgataac
     4741 tgccagttac tacgtctacg atacaaataa aattaaatct ggcgcactgg aggcaaccgc
     4801 attaatcaac gtgaaatacg actaataaca agcaagacga gcaagtggct aataataaaa
     4861 aataacaagg tgtctgcatt tccccctcat gatgaggggg ctttttttag cgatgataaa
     4921 aaatctcacc gtcgtaggct ttaatgattt taccgtcggt gtcgctgatc aacacgtacg
     4981 cgccacccat ataggtccag tgcgtcccgg catcaggcgc gggcaggtta cgtaactgcc
     5041 actgcttaat gttgtactca tcggtgcggt acatctctgg gacggtatcg cctggtttga
     5101 aatgcttaaa gtcagcaatg aaactgctta actcatactt agaaattccc gaggtattgg
     5161 tcgaacccgc ggtggctggt gcggcccagg cggcactggt aaccagcaaa agcgcaccca
     5221 gcaacatctt atttttaatg gtcataaact ctccactttg gggatctgta atatcagtcg
     5281 catatctttc ctgccataca atataactga caagcgatat tttgttagtg atcccatgat
     5341 ttcgtaaaaa gtgtaaatga aaggatgccc taaaagcatc cttagcgagc agagagtcag
     5401 cggaaatagc tgatttaaga atcatcctgg gctgaagata acatgcaagc ctaaaggatt
     5461 gagttatcgc attatgccca tgaagccatg tacgcccata tacacaccaa ctaagccaat
     5521 caacagactg gaaaaatagg gggcgcgttt agcgagagtg ttaaatccgc tccagcgttt
     5581 tgcgacctgc tgaacgctga ttgctgcgcc aacgcctacg gtgacaagcg ttaacgccag
     5641 gccaatgctg aaactgacga ccagtgtggc gcccagtgtc agggctttca actgaatgca
     5701 aatcaacagc acggtaattg ctgccgggca ggggataagg ccaccggtta agccaaatag
     5761 caaaatttgc cagttggtga cctctctacc atcaaagcgt cgtttaatgt cattagcatg
     5821 ggctcgtgca tgagcatcct gatactcgtc atgttcgtga tggtgatgat gtccatggtc
     5881 gtggtgatct tcgtgatcgt gatgatgatg ctcatgatca tgctcgtgca tattctccag
     5941 ccagttgcgc tcgccgtgcc aggtacgcca gaacatccag aacgcggtgc taataatgat
     6001 cactgcggaa atcaactgga gccacggttc tgctgattga gcagtaaagc gcttgctgac
     6061 caccatcccg ccaaaggcaa tcaaccagac cactgcggta tgcgaaatag ttgctgccag
     6121 tccgagcatc accgcctgtt taatggtgcc tttgatggcg atgataaacg ccgccatcat
     6181 cgtttttgag tgccctggtt ccaggccatg aagcgcacca agtaagatgg cgctggggat
     6241 gaagaaccag gcgtttcctt gctgaagaag agttgtaaat tcggtcatga taataattct
     6301 tagtattaat tcggcaatct gattctactc ccccccagta cctgatacta ccccccagta
     6361 gattaatagt gctatgattt ttcatgttct tgttaacaag gtgttgccat gtctcataca
     6421 atccgtgata aacagaaact gaaagcgcgt gccagtaaga ttcagggtca ggtcgtggcg
     6481 ctcaagaaaa tgctcgacga gccgcacgaa tgcgctgcag ttttacaaca gattgctgct
     6541 atccgtggcg cggtaaacgg tctgatgcgg gaagtgatta aaggtcatct gacggaacac
     6601 atcgttcacc agggggatga gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg
     6661 ctggattcat atatcaaata atttattaac gcgattgtaa aactgccgtt tttcctcgtt
     6721 tacaacgcgt gcgctggaca ttaccatcct cctctgcgat ttatcatcgc aaccaaacga
     6781 ctcggggtgc ccttctgcgt gaaggctgag aaatacccgt atcacctgat ctggataatg
     6841 ccagcgtagg gaagtcacgg accaccaggt cattgcttct tcacgttatg gcaggagcaa
     6901 actatgcaag tcgacctgct gagttcagcg caatctgcgc acgcgttaca cctttttcac
     6961 caacattccc ctcttgtgca ctgcatgacc aacgatgtgg tgcaaacctt taccgccaat
     7021 accttgctgg cgctcggtgc atcgccagcg atggttatcg aaaccgaaga ggccagtcag
     7081 tttgcggcta tcgccagtgc cttgttgatt aacgttggca cactgacgca gccacgcgca
     7141 caggcgatgc gtgctgccgt tgagcaagca aaaagctctc aaacaccctg gacgcttgat
     7201 ccagtagcgg ttggtgcgct cgattatcgc cgccattttt gtcatgaatt tttatctttt
     7261 aaaccgacag cgatacgtgg taatgcttcg gaaatcatgg cattagctgg cgttgctaat
     7321 ggcggacggg gagtggatac cactgacgcc gcagctaacg cgatacccgc tgcacaaaca
     7381 ctggcacggg aaactggcgc aatcgtcgtg gtcacaggcg aggtggatta tgttaccgat
     7441 gggcatcgta tcgttggcat tcacggtggc gatccgctaa tgaccaaagt ggtaggaact
     7501 ggctgtgcat tatcggcggt tgttgctgcc tgttgtgcct taccaggcga tatgctggaa
     7561 aatgtcgcat ctgcctgtca ctggatgaaa caagccggag agcgcgcagt cgccagaagc
     7621 gaggggccag gcagttttgt tccacatttc cttgatgcgc tctggcaatt gacgcaggag
     7681 gtgcaggcat gaaacgaatt aacgctctga cgattgccgg tactgatccg agtggtggtg
     7741 cggggattca ggccgatctt aaaaccttct cggcacttgg cgcttatggt tgctcagtta
     7801 ttactgcact ggtggcgcaa aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg
     7861 attttgtcgc cgcccagctc gattcggtgt tcagcgatgt gcgaatcgac accactaaaa
     7921 tcggtatgtt ggcggaaacc gatattgttg aagcggtggc agaacggttg caacgttatc
     7981 agatccaaaa cgtggtactc gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt
     8041 caccttcggc ggttgctacg ctgcgcagtc gattattgcc acaggtttca ttaataacgc
     8101 caaacttgcc cgaagctgcc gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa
     8161 tgctggaaca agggcgatcg ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg
     8221 gtcatctgga tgatgagcaa agcccggact ggctgtttac ccgcgagggt gaacaacggt
     8281 ttaccgcacc gcgcattatg accaaaaaca cccacggcac tggttgtacg ctctctgcgg
     8341 cgttggctgc actacgcccg cgccatacaa actgggctga caccgtacag gaggcaaaaa
     8401 gctggctttc atcagcatta gcccaggccg acacgctgga agttggtcac ggtattggtc
     8461 cggttcacca ctaccacgcc tggtggtgag caccgtacta tagctgcttt ggttattgtc
     8521 agcctttggc actgacattc agggatgaaa ccaatgcagc taagaattac cagtcgaaaa
     8581 aaactcacct ctctattatg cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt
     8641 cagacggtga attttttcta ttcgacagca gttcagatta cagactacat ccatttctac
     8701 ggttatcgcc cggttaaatc tttcgctatt cgcattcctg ccagttacac cattcacgga
     8761 atagatgttt ctcgctggca ggagcggatc gactggcagc gggtggcaaa aatgcgcgac
     8821 aacggtatcc gcttacagtt tgcttttatt aaggcgacgg aaggcgaaaa gctggtggac
     8881 ccgtattttt cgcgtaactg gcaactaagc cgcgaaaatg gcctgctgcg cggggcgtat
     8941 cattatttct ccccgtcggt agcagcgccg gttcaggcac gattatttct gcaaacggtg
     9001 gatttctcac aaggcgattt ccctgccgtg ctggacgtag aagaacgggg aaaattatcg
     9061 gcaaaagaat tacgcaagcg ggtaagtcag tggctaaaaa tggtagaaaa acgcacggga
     9121 aaaaagccga ttatttactc aggagccgtt ttttatcaca cgaatctggc gggctatttt
     9181 aatgaatatc cgtggtgggt ggctcactat tatcaacgtc gtccggacaa tgacggaatg
     9241 gcctggcgct tctggcagca ttccgaccgt ggacaggtag atggcatcaa tggtccggtg
     9301 gattttaatg tgtttaatgg cacggtggag gagttgcagg gattcgttga tgggattaaa
     9361 gaaacgcctt aaataagaag cagtgatata ctcccaccag actccctggc aggaaaaaga
     9421 caaatggaac aagcgcatac ccagctgatt gcccaactga acgagcgtat tttagcggcg
     9481 gataacacgc cgctttatat taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg
     9541 gtgctggagc atggcaatat tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt
     9601 tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag aagagggtgt ggtgacgcgt
     9661 tcgcgcggtt acggcacgca gatcaacaac atcttcgaat actcgttgaa agaagcgcga
     9721 ggtttttctc agcaggtggt attgcgcggg aaaaagcccg atacgctatg ggttaacaag
     9781 cgtgtcgtga aatgtcccga agaagtcgcg cagcagctgg cggtcgaagc aggaagtgat
     9841 gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg
     9901 tgggttccgg cgcatttaat tcatgatgtt gatgccatcg ggatttcact ttatgactat
     9961 ttccgcagcc agcacattta cccacaacgt acacgttccc gcgttagcgc ccggatgccg
    10021 gatgccgagt ttcagtcaca tattcagtta gatagcaaaa taccggtgct ggtgatcaag
    10081 caagtggcgc ttgaccaaca gcaacggcct attgagtaca gcattagcca ctgtcgcagc
    10141 gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc
    10201 ccgatgcccg acaacccacg acgccactgc attgcccagt aatacggcat ccgccagtgg
    10261 cagaccagag gccagcccgg caagtacgcc acctgcatga ctgtcgcccg ccccaatggt
    10321 gtctacaact tgcgttggaa atgccggaat gcagccagaa gcgtcgttgc tgaaatacca
    10381 tgcaccctct ttatcgagac gaacgatcaa cggcgcggca aatttctcct gccattgctt
    10441 gccaagtgtt gttatctctg cggataaagc aaaacgttcg gcggcaatct cagcctcttg
    10501 acgattgagc gacactaaag gtcgacaggc catgatccgc gccagtaatg catccgggat
    10561 atcgccaata cgtgggccaa aatcgataaa cggcgtcacg tcttgcagct cttccagcca
    10621 ttccactaac aattcgccgc agggcgaggc cagttgataa ccggaaaaat agagcaggct
    10681 gccaggcgca acggttaatc gcgccagcca ctggcgattc cactgatttt ccacaccgct
    10741 gaatgacata aaagtgcgtt cgccatccgg ctcaaccagc gccagacacc agccgttatc
    10801 accttcggcg ttatcgatca ggctgattaa gccctctttt gccatccggt tgcgaataat
    10861 ctccgcccac acgccctgac cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt
    10921 taacgccacg gcaatattca gtgcgcagcc gccaacgtta acgctctgct gtttcagttc
    10981 gatatcgcac ccacgccagg ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc
    11041 gccgacgacc atcaccggct gacgcgtggt taattcaggc agcagcgtgt gtaatcgagc
    11101 gccgctcata ccacctcccg ttgttgacga tatttcgcca acgctgtggc atagcggttg
    11161 aaatcaagct gatttaccgc atccagttcc gcctttaacg caggatcgat agcgttaacg
    11221 ccatgcaacg cgccgcaaat tgccgtcgcc atagcaccga tggtgtctgt gtcgccgcca
    11281 aggttagcgc acaggacggc gcagcgattc gggtcggttt gtgccagttc aaccagcgca
    11341 atggcgcacg gaacggactc aatagtgctg gtacctgcgc caacgacctg gtaaagctgt
    11401 tcgctggcgg attcggtgcc gtcggcattg cgcacaattt tcagcgcaat ctccagccgc
    11461 gccgccagtg aggcgctgaa cgtggtggtg cgtttttgct gggcatgtcg cgcaattgaa
    11521 ggcagtgaat caacaatcgc tgaccagctt tctccgtcaa tggcacgaga aatcgcccat
    11581 gcgatgacta ccgcgcctgc aaccgccaga tcggatttat gggtcgggct ggacgccagc
    11641 gctacatcat caataaagga atcaacatca cgcgccggaa gcaaacaacc taatggcgag
    11701 acgcgcatcg ctgcgccgtt ggtcacgccg ttgttttcca gttcagcaac gggtttaccg
    11761 tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccta gtacgttttt gttaaaggcg
    11821 tcgaaacgca gcgcccagtc gagaatatta cgcccaatca gatccggatc gatcttgcct
    11881 tcacgttcca gtaacgcatc cgccagacac agcgccatcg aggtatcgtc ggtgaattcg
    11941 gcgcggttaa aataacaggc cgcgttattc tcctttggtc caggaagaaa acggtcaatc
    12001 cagccaaagt gtgctttaac gcggctgcgt ggccaaagct cggagggcat ccccatcgca
    12061 tcccctaacg cctgcccata aagagcaccg agaatacgtt ctgttttcat ttaacttccc
    12121 cttgtgtcaa cgcaatatcg cgatcatcga ccttgatagc cgtaatttcg ttgtcggatt
    12181 cgcggaaaaa aatcatgaac agcacggcga taatggcaat catcaccgcg ccaaaagtcc
    12241 acatcccgga ccagttgaaa gtcagtccgt ttaccggttc ctgataagcg aacatctttt
    12301 ccatcatcac accgccaaga cgatagccga gcaaactgcc gaagccctgg cagcagagcg
    12361 tgatcagccc ctgcgcggcg gtacgcatat gcacgggggc ttttttatcg acatagatgt
    12421 aagcggtaac gtagtaaaaa tcgtaactta cgccgtgaag caaaataccg aggaacagta
    12481 acgcgtaggt gaaatattca tccgcactac cgtaaataaa gaagccatag cggatcgcag
    12541 cggtgaccag accaagcaat aatacctttt tgataccaaa gcgtttagtg aaaaacggta
    12601 atgccagcat aaagaatatt tcagagaact ggccgagcgt catccagccg gtggcatttt
    12661 tcatgccgac ttcggtcaga taaccgttgg caaagatgta atagaacgcc agtggcatcg
    12721 caaacaggaa tgaacagaag aaaaagacga ggaagttttt atcgcgcagc aggatcagcg
    12781 catccaggcc gagcatgact ttaatatcca ttttgccggt gctttttggt ggcgtgtcgg
    12841 gcaggaaaaa cgcaaacaca ccgagcagag cagaacttcc ggcggtaatc agcagcggga
    12901 tgttagtcgg tgagataacg gcatacccca gtatttgcgg caagaaacca catgccagac
    12961 cggaggcaat ccagccgata gtgcccatca cacgaatgcg cgggaaatca cgctccacat
    13021 ccggcacgtt ggcaaaagcg atgctgttag tcagcgcaat ggtcggcata taggttagag
    13081 agtaagccag cagtaacgga aagaacccgg caaaagtggt ctgttgcgca gcgaaataca
    13141 tcagcagcgc gcctgcgaac atcaataccg ccagcacttt ttgcgccgag aaaaagcggt
    13201 cagtgatgga gccaaccaga attggcgaca ggatcgccgc aatggcggta caggcatacg
    13261 accagccaat ttctccggca ctaaaaccgc ttttacttaa ccacaaccac aatggcacaa
    13321 accacgcgcc ccagataaac cattcaacaa acatcatgaa cgacagcttt gctgttgttt
    13381 tcatctttta attccttcat gacaggtaat gtaaggtacg cgatgacaat accacttaat
    13441 aatacctttt aaataccttt gcggtgaaaa cttgaccgcg ctaacatttt ttctgatgaa
    13501 tcgagccaac agaaaacgct gaaaaaacat ccaaaagatg gaaaaactcg cccgtaacga
    13561 ccaggattat gattgcccgc aaaaggcggg ccaggaaaat ccatagccga tatccaatcg
    13621 gaatttacgg gagcatagta atgacagata ttgcgcagtt gcttggcaaa gacgccgaca
    13681 accttttaca gcaccgttgt atgactattc cttctgacca gctttatctc cccggacatg
    13741 actacgtaga ccgcgtgatg attgacaata atcgcccgcc agcggtgtta cgtaatatgc
    13801 agacgttgta caacaccggg cgtctggctg gcacaggata tctttctatt ctgccggttg
    13861 accagggcgt tgagcactct gccggagctt catttgctgc taacccgctc tactttgacc
    13921 cgaaaaacat tgttgaactg gcgatcgaag cgggctgtaa ctgtgtggca tcaacttacg
    13981 gcgtgttggc gtcggtatcg cggcgctatg cgcatcgcat tccatttctc gtcaaactta
    14041 atcacaacga gacgctaagt tacccgaata cctacgatca aacgctgtat gccagcgtgg
    14101 agcaggcgtt caacatgggc gcggttgcgg ttggtgcgac tatctatttt ggctcggaag
    14161 agtcacgtcg ccagattgaa gaaatttctg cggcttttga acgtgcgcac gagctgggta
    14221 tggtgacagt gctgtgggcc tatttgcgta actccgcctt taagaaagat ggcgttgatt
    14281 accatgtttc cgccgacctg accggtcagg caaaccatct ggcggcaacc atcggcgcag
    14341 atatcgtcaa acaaaaaatg gcggaaaata acggcggcta taaagcgatt aattacggtt
    14401 ataccgacga tcgcgtgtac agcaagttaa ccagcgaaaa cccgattgat ctggtgcgtt
    14461 atcagttagc taactgctat atgggccggg ctgggttgat aaactccggc ggtgctgcag
    14521 gcggtgaaac tgacctcagt gatgcagtgc gtactgcggt tatcaacaaa cgcgctggcg
    14581 gaatggggct gattcttgga cgtaaggcgt tcaagaaatc gatggctgac ggcgtgaaac
    14641 tgattaacgc cgtgcaggac gtttatctcg atagcaaaat tactattgcc tgatacgcct
    14701 tatacgaaca gcaccacccc gtaggacgga taaggcgttt acactgcatc cggcaaccta
    14761 tgcctgacgc gacgcggaag cgtcttatca tgcctacata gcactgccac gtatgtttac
    14821 accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg gaggccgttt
    14881 ttttgccttt cactcctcga acaattttca tattgtcgtt tttgtgatcg ttatctcgat
    14941 atttaaaaac aa
//
LOCUS       DAZFRN010000123        14610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFRN010000123 DAZFRN010000000
VERSION     DAZFRN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.125"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(195..371)
                     /locus_tag="ACHKUB_003639"
     CDS             complement(195..371)
                     /locus_tag="ACHKUB_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO6656602.1"
                     /translation="MVINVKIKCAYHLCNKETEQTKSVKSHLHFMKGTIPVTEERQYC
                     SKQCAENDQMAHEL"
     gene            complement(365..586)
                     /gene="kil"
                     /locus_tag="ACHKUB_003640"
     CDS             complement(365..586)
                     /gene="kil"
                     /locus_tag="ACHKUB_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6656603.1"
                     /translation="MIAHHFRTDEIPRQCVTPGDYVLHEGRTYIASANNIEKRKLYIR
                     NFTTKTCITDCMIKVFLGRDGLPVKAESW"
     gene            1047..1256
                     /locus_tag="ACHKUB_003641"
     CDS             1047..1256
                     /locus_tag="ACHKUB_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656604.1"
                     /translation="MTQEEKVMFLMRLAVDTYNTQFREKDIPQKAVPAAVDKGGAIAV
                     FYDAFESFFDEKLDAVSDFGTSSNK"
     gene            complement(1225..1584)
                     /locus_tag="ACHKUB_003642"
     CDS             complement(1225..1584)
                     /locus_tag="ACHKUB_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656605.1"
                     /translation="MNETELKHIIALLLEDAKQVYRLSPNSATLTRIQMAEKALKQDN
                     ENSASEVDANGENEVIEINSNISDSCVAYSHQIIRVSARIMEVMASELEKNNIKPTDC
                     CLRTVMNVIYYSMFRSR"
     gene            complement(1616..2329)
                     /locus_tag="ACHKUB_003643"
     CDS             complement(1616..2329)
                     /locus_tag="ACHKUB_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656606.1"
                     /translation="MTTPSVLPQKLWRPLAEIKNFVEKMPDGVRLTEVTKKVKTFAEL
                     SGKERNQLIDFIDKRESIIVFKVRKEGSGNGVTFFRHKKYGYPKREGNVTIIKDLQSK
                     LCTRCGQTKSVDDFYSDASKRDGRAIYCKKCESAMKRSRRECNKLILQQQEPEMNNLK
                     AVSPSPEILRKQAEELLKAAEIAEKKRQEDDVFNKKLAPLKLEILQAAGKMQLKLDEF
                     IDCMDEMNKAVQKLKELTA"
     gene            complement(2384..2455)
                     /locus_tag="ACHKUB_003644"
     CDS             complement(2384..2455)
                     /locus_tag="ACHKUB_003644"
                     /inference="COORDINATES: protein motif:HMM:NF022510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HFO6656607.1"
                     /translation="MVWAIRTTAMIARELSKQKNKAA"
     gene            complement(2683..3021)
                     /locus_tag="ACHKUB_003645"
     CDS             complement(2683..3021)
                     /locus_tag="ACHKUB_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6656608.1"
                     /translation="MLMVKKTIYVNPNRGQNRKVSDRSLTSRDRRRIARWEKRIAYAL
                     ENGITPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIKDEKYEDIMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(2990..3328)
                     /locus_tag="ACHKUB_003646"
     CDS             complement(2990..3328)
                     /locus_tag="ACHKUB_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO6656609.1"
                     /translation="MVTIIWKESKGTAKSRYKARREELIAERRSSERLTRKIALKISG
                     CVRADKAASLGSLRNKKAEEVERKNRSTYYKDSNPLGNKIHAVQKIKLYSKPPYGLIE
                     YAYGEKDYLC"
     gene            3674..3883
                     /locus_tag="ACHKUB_003647"
     CDS             3674..3883
                     /locus_tag="ACHKUB_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656610.1"
                     /translation="MLIEGEFGVLHQRVVEILGVSLLEVIATGEAISADAIAGMIRVL
                     HHDELDDLAVKLAIDVLLQDMRLCN"
     gene            complement(4036..4743)
                     /locus_tag="ACHKUB_003648"
     CDS             complement(4036..4743)
                     /locus_tag="ACHKUB_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6656611.1"
                     /translation="MKTFAERLNAAMSSAGLSQAQLADMVGVSQPAIQKMSSGKTTGS
                     RKMVELANALRVRPEWLSSGIGSMRPQSAEEPSNARESSLKAIAWDDHQNDNDEFVAL
                     PLLNISLSAGGGSCALEESAEFSLVFRRYYLKKMGVPEKAAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTSIRDGKTYAICQADLLRVKTLIATPTSVIIRSINREEYPDEVMNREEF
                     YKNVKIIGRVFWSSHSW"
     gene            4854..5078
                     /locus_tag="ACHKUB_003649"
     CDS             4854..5078
                     /locus_tag="ACHKUB_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6656612.1"
                     /translation="MNLVIQRALNIVGSQKRLAADCGVSQPAVHKWLRGGKVSPEKVF
                     AIVNATNGQVKAYEIRPDLPHLFPHPNQAE"
     gene            5194..5490
                     /locus_tag="ACHKUB_003650"
     CDS             5194..5490
                     /locus_tag="ACHKUB_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO6656613.1"
                     /translation="MSQTSYSKLSQRDIDRAETDLLINLSVLTQRGLAKMIGCHESKV
                     SRTDWRYIAAILCAFQMASDISPISRAFQHAIRVHANKKRPVGAGRSEQILMNI"
     gene            5505..5723
                     /locus_tag="ACHKUB_003651"
     CDS             5505..5723
                     /locus_tag="ACHKUB_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656614.1"
                     /translation="MEQYTGIILPHLEEFRQQQHQPQQPEADWVNPEIPGPSVKMCSH
                     TNVQSHQPQQPEADWVNPGDTGTVCGME"
     gene            5744..6820
                     /locus_tag="ACHKUB_003652"
     CDS             5744..6820
                     /locus_tag="ACHKUB_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6656615.1"
                     /translation="MRNYATISPQFWLGDTGRKLRKSGPECMVVALYMMTSPHSNMLG
                     LYYLPVLYIAHETGLDPEGASKGLQMACEAGFCSYDHDSEVVWVHEMAAWQVGESLKP
                     GDNRCAGVRNEYSALLENPFLSSFYDRYKDDFHLDVRRESCRKIEAPSEPLSSQEQEQ
                     EQERDKTLLVHGEKIATDPLVDSCPVLTERPGPAGMTPEADSGRCVQQVLLVEPEQQH
                     QPQQPEADSAMSGKPIGLTRAMPGPSAGRVDYPDVFERVWREYPHRAGSNPKKSAFNA
                     WRARLREGVSPDVVLDGVRRYARYLEATGKAGTEFVQQASTFFGPNRNFENPWSLPKA
                     GAVSLRCVNHISEPDTEIPPGFRG"
     gene            6827..7567
                     /locus_tag="ACHKUB_003653"
     CDS             6827..7567
                     /locus_tag="ACHKUB_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6656616.1"
                     /translation="MKNISTGGILERVRRLAPPHVAAPFRTTDEWREWQLAEGRKRSE
                     EVNRQNHQTRVEKILNRSGIQPLHRKCSFGNYRVQNDGQRHALSLAKSIAAELHTGCT
                     NFVFSGKPGTGKNHLAAAIGNWLMAKGRSVIIVTVSDVMSVLHDGYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVILNQIVDRRTASLRSVGMLTNLNHAAMSTLLGERV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            7593..8363
                     /locus_tag="ACHKUB_003654"
     CDS             7593..8363
                     /locus_tag="ACHKUB_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6656617.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESGVTEGQALQPEAPDVVTEEVAPKVSADMMIEFIAQEGAKTCEEI
                     AGKFGVTTRKVASTLAVVTATGRLVRVSIKGKFRYKVPGVENGDLSASSSQDEKSHHE
                     AENSASGGELNRSPLMSEGTAKACTDEFIQDVPSFTEKYAGAVPLPPLRAASRELRRA
                     KKQVQKWERVCAALRELNKHREIVMQITDSSSSIASEK"
     gene            8378..8821
                     /locus_tag="ACHKUB_003655"
     CDS             8378..8821
                     /locus_tag="ACHKUB_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6656618.1"
                     /translation="MAKVFTPEEREKIKEQIVEFVRQSGRETLRALEAKTGASRYYIN
                     TLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKAKKPVVVDPGLIRALPDG
                     EIRRYDRRYNTICRECRNSEAMQRVLAFYRRCAQANNSLHSRVLA"
     gene            8834..9334
                     /locus_tag="ACHKUB_003656"
     CDS             8834..9334
                     /locus_tag="ACHKUB_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656619.1"
                     /translation="MHLNKEHQSMMGKRQTEEAVRDLEKLLGYRETKLLFLVPVIRQK
                     VEEILEENGIPKEYMTDAMEYSLELVRITPDLTWLDEYQQQFEKGAETLLSAADKTEQ
                     KTEPSVFWNTVEAIARSKIFEFNNINHSGRDTHQSSHLEGVSMLLALLREQGECPQST
                     LNDTEQ"
     gene            9637..9894
                     /locus_tag="ACHKUB_003657"
     CDS             9637..9894
                     /locus_tag="ACHKUB_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656620.1"
                     /translation="MNNKINIPLEIKEINEIIEVTDRPEFTLMHRYETGTDEQKYIMV
                     AALAVMAIERERREKDVMVIPARNDSPDLRWQEPEWDVAIR"
     gene            complement(9945..10304)
                     /locus_tag="ACHKUB_003658"
     CDS             complement(9945..10304)
                     /locus_tag="ACHKUB_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HFO6656621.1"
                     /translation="MKTVTIRIESMDAFTADVLSAFKAVAEGMTSKNESIVSFPDWQM
                     MHKVLTPKRMDILMAMTGAGELSIREIAALVGRDVKAVHTDVTALISNGLLEKGENGT
                     SFPYDDIHFDFTLGKAA"
     gene            complement(10306..10593)
                     /locus_tag="ACHKUB_003659"
     CDS             complement(10306..10593)
                     /locus_tag="ACHKUB_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HFO6656622.1"
                     /translation="MPSRLYKEFIRHLGESEYIRIKIWEVAPAVLGSRHNFKYSMAYV
                     VDGVCVMRYDNERGKGDHKHIGAQEISTHFVSIEQLIADFLHDVESIRRGA"
     gene            10942..11607
                     /locus_tag="ACHKUB_003660"
     CDS             10942..11607
                     /locus_tag="ACHKUB_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO6656623.1"
                     /translation="MTTQISVETISPITHNQIPVITTELLAQLYGTETNNIKVNYTRN
                     AERFVCGKHYFKLEGAGLREFKNKVTQSNLVAPRTKHLILWTERGAARHAKMLETDQA
                     WEVFEKLEDCYFSQGKTTQTEQQPQIQPQFTAEEIILLCYMQLWMEKAQDISKHLYPI
                     MKELNSSYTNKLYDIAFETIYMVTKNRDALLREVTRLDMSSSVIQRAMPMLKSLRARQ
                     FEF"
     gene            11864..12508
                     /locus_tag="ACHKUB_003661"
     CDS             11864..12508
                     /locus_tag="ACHKUB_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFO6656624.1"
                     /translation="MSTQLVFKSHVLETIEHDGKQWFTAATLASALEYSRGDKITQIY
                     NRNSDEFTPCMSINLKMRFNGINNSLREKDVRIFSLRGAHLVAMFATTPVAKEFRRWV
                     LDILDREAEKSAIPLQPHIQPQFTAEDIILLCYVQLWMEKAQQLSKQLYPVMRELNSD
                     YYGKLFDLAYETRHITNRTRDTLLREAAKLDPANYMVHRARSMLAQLRARQFEF"
     gene            12563..12973
                     /locus_tag="ACHKUB_003662"
     CDS             12563..12973
                     /locus_tag="ACHKUB_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO6656625.1"
                     /translation="MNQTIFLRSKQQQQFAINAILATTLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMVADISRQVQWCGRWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTSWAVEQGVRFTDRRYE"
     gene            12970..13161
                     /locus_tag="ACHKUB_003663"
     CDS             12970..13161
                     /locus_tag="ACHKUB_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6656626.1"
                     /translation="MRRQRRSITDIVCENCNYLPTKRSRNKPRPIPNESDVKTFNYTS
                     HLWDVRWLRRRARFNSHSG"
     gene            13185..13475
                     /locus_tag="ACHKUB_003664"
     CDS             13185..13475
                     /locus_tag="ACHKUB_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6656627.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGRGMK
                     PDDLLAAWACSDCHNEIDRRTRILDNNDARLYHLEGVIRTQAILLKEGKIKS"
     gene            13472..13834
                     /locus_tag="ACHKUB_003665"
     CDS             13472..13834
                     /locus_tag="ACHKUB_003665"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6656628.1"
                     /translation="MNEYEFVLPWPPTVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LRLDIFTKSRLRITIIAEPPDSRRRDLDNILKGLLDSLIYAGFAEDDEQFDDIRVIRG
                     VKVPGGRVGIKITELENI"
     gene            13834..14028
                     /locus_tag="ACHKUB_003666"
     CDS             13834..14028
                     /locus_tag="ACHKUB_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO6656629.1"
                     /translation="MNAKIQTIPELLICTRGNQTEVARILNCNRATVRKYIDDKDAKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            14021..14455
                     /locus_tag="ACHKUB_003667"
     CDS             14021..14455
                     /locus_tag="ACHKUB_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6656630.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
BASE COUNT         3885 a         3258 c         3536 g         3931 t
ORIGIN      
        1 gtcgattttg tattctttgc atattttact gatgcggttt gcaatttcat taagttcatt
       61 tactgctgtc ttttcggttt cagaaaagat ttggtcagga ttaagttctt tcactttata
      121 tgtccttttt ttaaggccta tggtgctgtt atatctggca tgaaactgaa ttgtctgaat
      181 atcaagttga ggggttagag ttcatgtgcc atctggtcat tttcggcgca ttgcttactg
      241 caatattgcc gctcttcggt tacggggatt gtgcctttca taaaatgaag atgactttta
      301 acggatttgg tttgttcggt ttccttattg cagagatggt aggcacattt tatttttaca
      361 tttatcacca tgactccgct tttacaggta aaccatcacg tccgaggaag actttaatca
      421 tgcaatcggt aatgcatgtt tttgtcgtga agttacgaat atagagtttt ctcttttcaa
      481 tattgtttgc tgaagcgata tatgtccgcc cttcatgaag aacataatcg ccaggtgtca
      541 cgcactgacg tggtatttca tcagttctga agtgatgagc aatcataatt atctccattt
      601 tcacaaatga actttgttga tgcggtgcct ggtgcctcca ggtgacgtta accagttaac
      661 aattaacgcc gggtcagggg acgatgactt tccgtgacat cctgtcggtt taactgttcc
      721 gcgtgcgctg agccgcattc accgcatcac aaaattcact ttaaaaaagg gcggatatca
      781 gccagcaatt aaactgatat ccgccaatga ctacacacag caatgttgtt attcacaacc
      841 ggaagcgcac ggtcgaagaa atctaacgac aagccttcaa agggaaagag tcttcgccgt
      901 gcgctttcgt gttgcaccct ggcttttcag ggtaatatct gttcagtaaa ctgacagtgc
      961 cggatgctca cccgtgtccg gcgcacgaac tccacctgac ccgtggagaa ctcctcaact
     1021 acaaaccccg taaggagagt gaatttatga cacaagaaga aaaagtgatg tttctgatgc
     1081 ggctggctgt cgatacctac aacacacaat tcagggagaa agatatacct caaaaggcag
     1141 ttcctgccgc ggtagataag ggcggtgcta ttgccgtatt ttacgatgca tttgaatcat
     1201 tttttgatga aaaactcgat gctgttagcg acttcggaac atcgagtaat aaataacgtt
     1261 cattacggtt cttaaacaac aatcagtggg cttgatgttg ttcttttcaa gctcacttgc
     1321 catcacttcc attattcttg cgcttacacg aattatttgg tggctgtaag cgacgcaact
     1381 gtcgctgata ttgctgttta tttctattac ttcattttca ccattagcat caacttcgga
     1441 tgcgctgttt tcattatcct gtttcagtgc tttttctgcc atctggatgc gtgtcagcgt
     1501 tgcggaattt gggctaagtc gataaacctg tttggcatct tccagaagca gggcgataat
     1561 gtgcttcagt tctgtttcgt tcatagttaa ctccggtagt tgcaatttat taatatcagg
     1621 cggtcagttc tttaagcttc tgaacagctt tattcatttc atccatacag tcgatgaatt
     1681 cgtccagttt aagctgcatt tttccggcgg cctgaagaat ttcaagtttt aagggcgcaa
     1741 gttttttgtt gaatacatca tcttcctggc gttttttctc cgcaatttcg gcggctttca
     1801 gcaattcttc cgcctgcttt ctgagtattt ctggtgaagg tgaaactgct tttaggttat
     1861 tcatttcagg ttcctgttgt tgcagaatta atttgttgca ttctctgcgt gagcgtttca
     1921 ttgcagattc gcacttcttg caataaatgg ctctcccgtc acgtttgctg gcatctgaat
     1981 aaaaatcatc gactgatttt gtctgcccgc atctggtaca taattttgat tgaaggtcct
     2041 taatgattgt gacgtttcct tcccgcttgg gatatccata ttttttgtgg cggaaaaagg
     2101 ttactccgtt accagaacct tcttttctga ccttaaatac aatgatgctt tcccgtttat
     2161 cgataaaatc tatgagctgg tttctctcct ttcctgacag ttcggcaaat gtcttaactt
     2221 ttttagtaac ttccgtaagg cgaacgccgt caggcatttt ttcaacgaag tttttaatct
     2281 ctgcaagcgg acgccataat ttttgcggca aaactgaagg cgtggtcatc aatcacctcg
     2341 ctttttttta ctgatttgtt tatgccaaaa ataaaggcca ccatcaggca gccttgtttt
     2401 tctgtttgct aagttctctg gcaatcatgg ccgtagttcg tattgcccac accattgatt
     2461 tgtatcaata gtcgtagtca tccggatagt cctggtattg ttccatcaca tcctgtggat
     2521 gctcttcgaa ctcttcaaat tcttcttcca tatctcactt caaataagtg gtttgctgcc
     2581 taattttatt ttctggcgac caacacaagt cacacccgtt tcactgcgtg gcttgcggta
     2641 gtaaattttg ttagtccaaa caataaaatc taccgaagtg ggttatgacc attttttatt
     2701 tggatttcgt tggtgagcgt ggttaacaac tctgtgcatt atatcctcat atttttcatc
     2761 tttaattttt tcaacatctc gaggaaatgg tgttgctaat gctttgtcaa ctttgtccat
     2821 tgggtcttca ttaatcttat attccggacc gtcatctata gcattaaatc caggtgttat
     2881 accgttttct aatgcatatg ctattctctt ttcccatctc gctatcctcc ttctgtctcg
     2941 agatgtaagg cttctatcag atacttttct gttttgtccg cgattaggat taacataaat
     3001 agtctttttc accataagca tactcaataa gtccatacgg tggtttactg tacaatttta
     3061 ttttttgaac tgcatgtatt ttgtttccta atgggtttga atccttgtaa taagtacttc
     3121 tgtttttgcg ctcgacttct tctgccttct tgttgcgaag gctgccgagt gatgctgctt
     3181 tgtctgctct gacgcaacca gagatcttta gcgcaatctt ccgcgtcagt ctttcactac
     3241 tgcgccgctc tgcaataagt tcttccctgc gagctttata gcggcttttt gccgtacctt
     3301 tggattcttt ccagattatg gttaccatga tggtctcctt taagtggctt tggtgtatga
     3361 cgcgtcgagg tgtttttctt ctcgatcgcg gccttgcaac tgaaattcgc gtcatcccca
     3421 aaaccactta gattttggtc tcaacggtta ggttgagagt ccatcaatgt taaagagcct
     3481 gccaatctgt tccgtttggc ttccagcgtc ctgctgttga attgaagata acctaagtta
     3541 tatgattgtg caataactat atttatcatt ttatggaaag cgttataaat ggttgataac
     3601 aaaaatattt tattttttgt ggtatccgcg tgatattttc aagggggaaa ggctgattgt
     3661 tatgggtgat tgcatgttaa tcgaagggga atttggtgtg ttgcaccagc gggtagtcga
     3721 aattctaggg gtgtcgttgc ttgaggttat tgctactggg gaagctattt cagcagatgc
     3781 tattgcggga atgatccgag tgcttcacca tgatgaattg gatgatctcg ctgtgaagtt
     3841 agctatagat gtgttacttc aggatatgcg actgtgtaat taagtaaata aaacccggca
     3901 ctggagccgg gggatttaga aatggcattt tatgaattgg ctacttggcg aatggtttac
     3961 catcggtggt attgagcaca ataaaaggcc gttcttcgtg agtgttctta tagcaaaagt
     4021 tcagttggag tctgattacc agctatgtga tgaccagaac actcgtccta tgatttttac
     4081 gtttttgtag aattcttctc tgttcattac ttcatcagga tactcttcgc ggtttatcga
     4141 tctgataatt accgatgttg gtgtggctat tagcgttttt acccttaaca aatcagcttg
     4201 gcaaattgca tatgttttac catccctgat gctcgtatct tgcgtgttta cccccaccac
     4261 atcaccatca tggagtgttg gctccatact ttgcccaaca accctgacca actttgccgc
     4321 tttttctgga actcccattt ttttcaggta atagcgccta aaaactagag agaattctgc
     4381 ggactcctcc aatgcacaac ttcctccgcc agctgaaagt gaaatattta gaagggggag
     4441 cgcaacaaat tcgtcattat cattttgatg atcatcccaa gcgatagctt ttaaagatga
     4501 ttcccgagca ttagatggct cttctgcgct ctgtggtctc attgacccta taccagagct
     4561 tagccactca gggcgaactc ttaacgcgtt ggctaattcg accattttac gtgatccggt
     4621 tgttttacca gatgacatct tttgtatggc tggttgtgat acccccacca tgtctgcaag
     4681 ttgtgcttgt gacaacccgg ctgaactcat ggcggcattt agtctttctg cgaatgtttt
     4741 catacctgta atctataacc acggttatca aaagtaaaac aacaattgtt attgctctgt
     4801 tgtataaccc atgttatttt tggttatgct ttatttgctg tagaggtatg ctcatgaatt
     4861 tagtgattca gcgagccttg aatattgttg gcagtcaaaa acgacttgca gctgattgcg
     4921 gcgtatcaca gcccgctgtt cataaatggt tgcgaggcgg aaaagtttct cctgaaaaag
     4981 ttttcgctat cgttaatgcc accaatggtc aggttaaggc ttacgaaatt cgcccggact
     5041 taccgcacct gtttcctcat ccgaaccagg ctgaataagt aacaccgctc tttaacattg
     5101 ctggtcgttc acctctaaca gggtgagcaa acatcagtgg caaacccatt gggattgccg
     5161 cttaacccca tatcaatata ggaaaattaa caaatgtcac aaacaagtta cagcaaactg
     5221 tcgcagcgcg atatcgatcg cgctgaaacg gatttactta tcaacctgtc agttctgacg
     5281 caaaggggac tggcgaagat gattggctgc catgaatcga aggtcagtcg taccgactgg
     5341 cgatacatcg cggcgatttt atgtgcgttc cagatggcat ctgatatcag tccgatcagc
     5401 cgggctttcc agcatgccat tcgcgttcat gcaaataaaa aacgtccggt tggggccgga
     5461 cgttctgagc aaatcctgat gaatatctga tattcaggca gggcatggag caatacacgg
     5521 gaataattct gccacatctg gaagaatttc gccagcaaca acaccaaccg cagcagcctg
     5581 aagccgattg ggttaacccg gagataccgg gaccgtctgt gaagatgtgc agtcacacca
     5641 atgtgcagtc acaccaaccg cagcagcctg aagccgattg ggttaacccg ggagataccg
     5701 ggaccgtctg cggtatggag taaatcttgt atgcgagggg actatgcgta attacgcaac
     5761 aatttcacct cagttctggt taggcgatac agggcgaaaa ctaaggaagt ctggtccgga
     5821 atgtatggta gtggcgttgt atatgatgac ctcgcctcat tccaatatgc tgggccttta
     5881 ttacctgcct gttttgtaca ttgctcacga aaccggactt gatcctgaag gggcttctaa
     5941 ggggcttcaa atggcttgcg aggctggttt ttgcagctat gaccatgatt ctgaggttgt
     6001 atgggtgcat gaaatggcag catggcaggt tggtgaatcg ctgaaacctg gcgataaccg
     6061 ttgtgctggg gtaagaaatg aatattccgc gttgctggaa aatccttttt tatcatcatt
     6121 ttatgataga tataaggatg atttccacct ggatgtcaga cgtgaatcat gtcggaaaat
     6181 tgaagcccct tcagagcccc tttcaagcca agaacaggaa caggaacagg aaagagataa
     6241 aacccttctg gtccatggcg aaaaaatcgc cacggatcca ctggtggatt cttgtcccgt
     6301 tctgactgaa cgtccaggac cagctggcat gacaccggaa gcagattccg ggcgttgtgt
     6361 gcagcaggtg ctgctcgtcg aaccggagca acaacaccaa ccgcagcagc ctgaagccga
     6421 ttccgcgatg agcgggaagc cgattgggtt aacccgggcg atgccgggac cgtctgcggg
     6481 acgagttgat tatcctgacg tgttcgaacg ggtctggcgt gaatatccgc atcgggcagg
     6541 gtcaaacccg aagaaatccg cgttcaatgc ctggagggcc agattacgcg aaggggtgtc
     6601 accggatgtc gtgctggatg gcgtgaggcg ttacgcaaga tacctggagg ctaccgggaa
     6661 agcgggaact gaatttgttc agcaggcatc gacgtttttt ggcccgaaca ggaatttcga
     6721 aaatccgtgg tcgctgccga aggctggcgc agtcagcctg cgttgcgtga atcacatttc
     6781 tgaaccggac accgaaattc cgccgggttt cagggggtaa tcagccatga aaaacatttc
     6841 gacaggaggg attcttgaac gggtgcgccg tctggcacca ccgcacgtgg cagcaccgtt
     6901 ccggacgacc gacgaatggc gggaatggca actggctgag ggccggaagc gcagcgagga
     6961 agttaaccgc cagaatcacc agacgcgggt tgaaaaaatc ctgaatcgtt cgggcatcca
     7021 gccgcttcac aggaagtgct cattcgggaa ctaccgggtg cagaacgacg gtcagcgcca
     7081 tgctctgagt ctggcaaaat ccatcgcggc agaactgcat accggctgca cgaatttcgt
     7141 gttcagcggt aaacctggca ccgggaaaaa tcaccttgca gcagcgattg gcaactggct
     7201 gatggcgaag gggagaagcg tgattatcgt caccgtgtcc gatgtcatga gtgtgttgca
     7261 tgacggctac gacaacggca agtctggtga gaaattttta caggaacttt gcggggtaga
     7321 ccttctggtc cttgatgaaa ttggcatgca gcgggatacg cgcaacgagc aggtcatact
     7381 gaaccagatc gtcgaccgca gaacggcatc actgcgcagt gtcgggatgc tgacgaacct
     7441 gaatcacgca gctatgagca cactcctcgg cgagcgggtg atggaccgta tgaccatgaa
     7501 cggtggtcgt tgggtgaatt ttaactggga gagctggcga tcaaacgttg ggcgtcaggg
     7561 tatgtgagaa tttttgacga ggtaaatttt cgatggaaac cgtattgcat gcactgaaag
     7621 cgatgggaaa agccaattct gttgaactgg cggcgcggct tgatatcagc cgtgaagaag
     7681 ttctcaacga actgtgggaa ctcaaaaaaa atggcgttgt tgataaaacg ggtcacacct
     7741 ggtttctggc tgtcgaaggt gaatccgggg taaccgaagg gcaggcacta caacctgaag
     7801 cgccggatgt ggtaaccgaa gaggtcgctc caaaagttag cgctgacatg atgattgagt
     7861 ttatcgctca ggagggggct aaaacctgtg aagaaatagc gggtaagttc ggcgttacca
     7921 ctcgtaaggt tgcttccacg ttggcggtag taacagcaac ggggcgcctg gtgcgcgtgt
     7981 ctatcaaggg aaaattccgt tataaagtgc ctggcgttga aaacggcgat ttgagcgcgt
     8041 catcatcgca ggatgagaaa tcacaccacg aagcagaaaa cagcgcgtca ggcggcgaac
     8101 tgaatcgttc tccgttgatg tcagagggca cggcaaaggc ctgtacagat gagtttatcc
     8161 aggatgttcc gtcgttcacc gaaaaatatg ctggtgcagt gccccttcca cctctacggg
     8221 ccgccagtcg tgaacttcgt cgcgccaaaa aacaggtcca gaagtgggag cgtgtctgtg
     8281 ctgcactacg tgagctgaat aaacaccggg aaattgtcat gcagattacc gattcatcca
     8341 gcagtattgc atcggaaaag tgataactgg agagcttatg gcaaaagtat ttacacccga
     8401 agagcgggaa aaaattaagg agcagatcgt tgaatttgtg cgccagagtg gacgtgagac
     8461 gttacgggct ctggaagcta aaactggtgc atcaaggtat tacataaaca ctctcgccag
     8521 agaactggtc gcaagtggtg atgtttacaa ttcaggttac ggattattcc cgtctgagca
     8581 ggcgcgtaag gactggcaaa atgcccgcaa aaaactctcg agagcaaagg caaagaaacc
     8641 ggttgtcgtt gatcccggtc ttatccgggc attacccgat ggggaaatac ggcgctacga
     8701 cagacgctac aacacaattt gtcgcgagtg ccgtaacagt gaagcaatgc agcgtgtgct
     8761 ggcattctat agacgttgcg cacaggcaaa taacagtctg catagccgtg tattggctta
     8821 acaggagcta aacatgcatt tgaataaaga gcatcaatcc atgatgggca agcgccagac
     8881 agaagaggct gttcgcgatc tggaaaaact acttggctat cgtgaaacaa aactgctttt
     8941 cctggttccg gttattcgcc agaaggttga ggaaattctt gaagaaaatg gcatcccgaa
     9001 agaatacatg acggatgcca tggaatattc actcgaactg gtacgaatta ctcctgatct
     9061 gacctggctt gatgagtatc aacaacaatt tgagaaaggc gcagaaacgt tactttctgc
     9121 tgctgataaa acagagcaga aaacagaacc atcggtattc tggaataccg ttgaggctat
     9181 tgccaggagc aagatatttg agtttaacaa catcaatcat tccgggagag acactcatca
     9241 atcatcacat cttgaggggg tgtccatgct tctagctctg ttgcgagagc aaggcgaatg
     9301 tcctcagtcg acattgaacg acactgagca atagtccatc cgtattttcc ggcaagataa
     9361 agatatattg cgtcaaagcc ataaatagtc cttggatacc cctctttaat agccagagag
     9421 tccccgaagc attcaagaaa gtaatttgag cgggctgttt cgtaatgaaa attccagcga
     9481 gcctgattga gttttttgtc catttatcct ccattgttgc tctgttttga aaaatggaga
     9541 ccttcacgct gcaacgtgag gtcgtgcgcc ggacacgggt aaacatccgg tattcaaaat
     9601 ttaatgttaa cgaattttta atattgaggt tatgacgtga acaacaaaat caatattcct
     9661 ctcgaaatta aagaaattaa cgaaatcatt gaggttactg atcgccccga gtttaccctc
     9721 atgcaccgtt atgaaacggg aaccgacgag caaaaataca tcatggttgc agcattggct
     9781 gtgatggcga tagaaaggga gcggagggaa aaggacgtaa tggttattcc ggcgagaaat
     9841 gattcaccgg atttgagatg gcaggagccg gagtgggatg tggcaatccg gtgattgata
     9901 tttctctggg gtaaagcgcc gccagaatga cggcgcggtg aattttacgc agctttaccc
     9961 agcgtaaaat caaagtggat gtcgtcatac ggaaaagatg ttccattttc tcctttttcc
    10021 aggaggccgt tgctaatcag tgcggttaca tccgtgtgta cggcttttac gtcacgcccg
    10081 accagagcgg caatctcacg gattgacagt tctccggcac cagtcatcgc catgagtatg
    10141 tccatgcgct taggggtaag cactttatgc atcatttgcc agtctgggaa tgaaacgatg
    10201 ctttcgttct ttgacgtcat tccttcggca accgctttaa aggcagacaa cacatctgcc
    10261 gtgaaagcat ccattgattc aatacgtatt gtaactgttt tcatatcagg cacctctgcg
    10321 aatggattct acatcatgga ggaagtcagc aatgagttgc tcgatggaaa caaagtgggt
    10381 actgatttcc tgagcaccaa tgtgtttgtg gtcgccttta cctctttcgt tgtcgtagcg
    10441 catgacgcaa acaccatcaa caacgtaagc catgctgtat ttgaagttat ggcggctacc
    10501 aagaacggct ggagccacct cccagatttt aatccttatg tattcggatt ccccaagatg
    10561 gcgaatgaac tctttataca gtctggatgg cataaaaact ccgtgtattt atgttgttaa
    10621 ggataacaac agtgcatagc gttgtcaatt aatacaacat caatgctgag ttgacgacaa
    10681 cacttatccg ggattatatt cttcgcacgg tcgagttggc cgtcgggatt ggaaccccgg
    10741 atagagaccg cgacaaacac acgccgcgag cgtgtttttt attgtcgtat gcacgcgcac
    10801 atctgaatta tggtggggcg catgggggag ctgaaaagct cgccggtcgg tttcccggta
    10861 gttccaaccc tgtgcgtctc accacccgat gattggaacc ttacggtggt gatagtttag
    10921 aaaccactag agggcgtcat tatgacaact caaatctctg ttgaaaccat ctccccgatt
    10981 acccataacc agattcctgt tattaccacc gagcttttgg cacaacttta tggcaccgaa
    11041 actaataaca tcaaagtgaa ctatacacgc aatgccgagc gttttgtttg tgggaaacac
    11101 tacttcaaat tggaaggggc tggattgcgg gaatttaaga acaaggttac tcaaagtaac
    11161 ttagttgcac cgcgtacaaa gcacctcatc ctctggacag aacgcggggc agcccgtcac
    11221 gcaaaaatgc tcgaaactga tcaggcatgg gaagtgttcg aaaaactgga agactgctat
    11281 ttcagccagg gaaaaacaac acaaaccgaa cagcagccgc agattcagcc acaattcaca
    11341 gccgaggaaa tcatcctcct ttgctacatg cagctctgga tggaaaaagc ccaggacatc
    11401 agcaaacacc tgtaccctat aatgaaagag ctgaactcct catacacgaa caagctgtat
    11461 gacattgcgt ttgagaccat ctacatggtg acgaagaaca gagacgcgct actgagggag
    11521 gtaacacgtc tcgacatgtc aagttccgtt atccagcggg ccatgccaat gctgaaaagc
    11581 ctgcgggcaa gacaattcga gttttaaggc cagatttgac aaatccgcat taacggggat
    11641 atattccgca ccaaggtgct gaacacacct atacaagcgg aagccgcacc cgtcagtcat
    11701 gcggattttt tatgtccatt tttcagatat ggtcgggtag cgcgtatacc gaaaaacagc
    11761 cgcaaggcta aggatacggg ccgttcttgt atgcggtgtt caagtacccg accgccctgc
    11821 tgaacacagg gattctgaac aaaaaataca aggacataaa actatgagca ctcaactcgt
    11881 attcaaatcc cacgttcttg aaacgatcga acacgacggc aaacagtggt tcactgccgc
    11941 gactttggct tcagcactgg agtattcgcg cggcgataaa atcacccaga tatacaaccg
    12001 caattcggat gagtttactc cttgtatgtc aatcaacctc aaaatgaggt tcaacgggat
    12061 caacaatagc ttacgagaga aagatgtgcg tattttctct ctacgcggtg cccacctggt
    12121 agcgatgttc gcaacaactc cagtagccaa agagttccgt cgctgggtat tggatattct
    12181 ggaccgtgaa gctgaaaaaa gcgctatacc gctacaacct catattcagc cgcaattcac
    12241 agcagaagac atcatcctac tctgctacgt tcaactctgg atggaaaaag cacaacaact
    12301 cagtaagcag ctataccccg tcatgagaga actgaactct gattactacg ggaaactgtt
    12361 tgatctggcc tacgagactc gtcatataac caaccggact cgcgacacat tgctccgcga
    12421 agcagcaaaa cttgatcccg ccaactatat ggtgcatagg gccagaagca tgctggcgca
    12481 actacgggca agacaatttg aattctgaaa ctaaaggagc ttcggctcct tttttgttgg
    12541 gaaaatccaa tgagagggaa taatgaacca gactatcttc ctccgaagta agcagcagca
    12601 acaattcgcc attaacgcca tccttgcaac aactctcgat aaagacaaac ccgttacgat
    12661 ccgcatcacc gattacaagc ggaatctcga tcaaaatgcc aaatttcacg cgatggtcgc
    12721 tgatatctcc cgacaggttc agtggtgcgg cagatggcta aaaccagaac agtggaaagt
    12781 tttgttaatc agcggtcatg ccgtggcgac aaaacaggaa gctgatgttt tgccaggtct
    12841 tgaaggtgaa tacgtcaata tccgcgaaag cagtgcgcag atgagcgtga agcgtatggc
    12901 aagcctgatt gagtacacaa cttcctgggc tgtggagcag ggtgtcagat ttaccgacag
    12961 gaggtatgaa tgagacgaca gcgacgaagc attaccgata tagtctgtga aaactgcaat
    13021 taccttccaa cgaaacgctc ccgaaataaa cccaggccaa tccccaacga atctgatgta
    13081 aaaaccttca attatacgtc tcacctgtgg gatgtccggt ggctcagacg tcgtgcaaga
    13141 tttaatagtc actccggata gttcaatgta cgaggaataa gatgatggca aacctacgca
    13201 aagaagcgcg tggcagagaa tgtcaggtac ggatttacgg cgtatgcaat ggcaatcctg
    13261 aaactacagt tttggcgcat taccgaatgg ctggaatttg cggaagggga atgaagccag
    13321 atgacctgtt ggctgcatgg gcctgtagtg actgccataa tgaaatcgat cgccgtactc
    13381 gcattctcga caacaacgac gccagacttt accacctgga aggcgtgatc aggacgcagg
    13441 cgatactgct gaaggagggg aaaattaagt catgaatgaa tatgagtttg tgcttccctg
    13501 gccgccgacg gtgaatacct actggcgaag acggggaagc cagtactaca tcagcgataa
    13561 aggccagaaa taccgaaaag acgtacagca aatcatccgg caactcagat tagacatttt
    13621 cactaaatca cgacttcgca tcacaattat tgctgaacca ccagattccc gtcgtcgcga
    13681 cctcgataac atcctgaaag gtttactcga ctctcttatc tacgccggat ttgcggaaga
    13741 cgacgagcaa ttcgatgaca ttcgcgtaat tcgcggcgtg aaagtgcctg gcggtagagt
    13801 ggggataaaa atcaccgaac tggagaacat ttgatgaatg ctaaaattca aacgatacct
    13861 gaattactga tctgcaccag gggaaatcag acagaagtcg ccagaatact gaactgcaat
    13921 cgtgccacag tcagaaaata cattgatgat aaagatgcga aaaagcacgc cgtcgtcaat
    13981 ggcgtcctta tggttcatcg cggatggggt aaagatactg atgcgtgata tccggcaggt
    14041 tcttgagcgc tggggtgcat gggcggcaaa taactatgag gatgttacat ggtcgcccat
    14101 tgctgccgga tttaagggac tgatccccga aaaagtaaaa tcacgtccac agtgttgtga
    14161 cgatgacgcg atgattatat gcgggtgtat ggctcgcctt aacaggaaca acagtgatct
    14221 gcatgacttg ctggttgatt attacgtgtt gggggagacg ttcatggcgc tggcacggaa
    14281 acatgggtgc tctgacacct gtataggtaa acgccttcac aaagcggagg ggattgttga
    14341 aggcatgctg atgatgctgg gagtgaggct tgagatggat cggtatgttg agcgtgaatt
    14401 gccgggaggg agaacctctg tattttatca gcgaaaaaat agtttacgat cgtaaaaatc
    14461 tgcatatcat gataagagtg gttacattgc tacgctgctt aacccgccga tgcgcgggtt
    14521 tttttgtacc cagaatcctg tgagctatac ggaaagtaca cagaaaggaa ggtgcgaccg
    14581 taattaataa caaaatctta aaaatcgcat
//
LOCUS       DAZFRN010000124        14381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFRN010000124 DAZFRN010000000
VERSION     DAZFRN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.126"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            26..592
                     /locus_tag="ACHKUB_003668"
     CDS             26..592
                     /locus_tag="ACHKUB_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656631.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            643..3102
                     /gene="ybgQ"
                     /locus_tag="ACHKUB_003669"
     CDS             643..3102
                     /gene="ybgQ"
                     /locus_tag="ACHKUB_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6656632.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3117..3848
                     /gene="ybgP"
                     /locus_tag="ACHKUB_003670"
     CDS             3117..3848
                     /gene="ybgP"
                     /locus_tag="ACHKUB_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6656633.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3845..4906
                     /gene="ybgO"
                     /locus_tag="ACHKUB_003671"
     CDS             3845..4906
                     /gene="ybgO"
                     /locus_tag="ACHKUB_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656634.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5058..6104
                     /gene="abrB"
                     /locus_tag="ACHKUB_003672"
     CDS             5058..6104
                     /gene="abrB"
                     /locus_tag="ACHKUB_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO6656635.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHKUB_003673"
     CDS             complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHKUB_003673"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO6656636.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHKUB_003674"
     CDS             complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHKUB_003674"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO6656637.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHKUB_003675"
     CDS             complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHKUB_003675"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO6656638.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHKUB_003676"
     CDS             complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHKUB_003676"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO6656639.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHKUB_003677"
     CDS             complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHKUB_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO6656640.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHKUB_003678"
     CDS             10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHKUB_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO6656641.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11794..13212)
                     /gene="phrB"
                     /locus_tag="ACHKUB_003679"
     CDS             complement(11794..13212)
                     /gene="phrB"
                     /locus_tag="ACHKUB_003679"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO6656642.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13209..13718)
                     /gene="ybgA"
                     /locus_tag="ACHKUB_003680"
     CDS             complement(13209..13718)
                     /gene="ybgA"
                     /locus_tag="ACHKUB_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO6656643.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13869..14381)
                     /locus_tag="ACHKUB_003681"
                     /pseudo
     CDS             complement(13869..14381)
                     /locus_tag="ACHKUB_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3462 a         3847 c         3492 g         3580 t
ORIGIN      
        1 ttcttttctg ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc
       61 cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca
      121 ctttaaaggt gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga
      181 ggttgaactc ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt
      241 tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg
      301 caagatttct aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc
      361 gattctcaac aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa
      421 agatcaaagc aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc
      481 cagcgaacaa acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt
      541 aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact
      601 ttggcggcgc tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt
      661 tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa
      721 ttcaacctta atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag
      781 gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa
      841 atcagtaacg ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc
      901 aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg
      961 ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc
     1021 aatcagcaac taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg
     1081 accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt
     1141 gccagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca
     1201 gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat
     1261 agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta
     1321 ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac
     1381 ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta
     1441 cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt
     1501 caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac
     1561 cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg
     1621 aacaatttcc aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc
     1681 tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc
     1741 ttttttagca atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg
     1801 ctgctttctg gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg
     1861 cttggcgcgc tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac
     1921 gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg
     1981 atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg
     2041 gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg
     2101 ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg
     2161 tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt
     2221 gactggagag atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat
     2281 cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc
     2341 tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg
     2401 gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga
     2461 gcccaggtga gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt
     2521 acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca
     2581 acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc
     2641 agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt
     2701 ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg
     2761 aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa
     2821 ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt
     2881 cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc
     2941 attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat
     3001 caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat
     3061 atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga
     3121 catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac
     3181 tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg
     3241 tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag
     3301 gggagaaaag cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag
     3361 caacaacgca ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg
     3421 aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc
     3481 atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat
     3541 tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc
     3601 aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg
     3661 aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca
     3721 caaacaccgg aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg
     3781 cgttacgcat gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga
     3841 agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa
     3901 gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga
     3961 agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc
     4021 tgacgatatt aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga
     4081 gcatgtttat gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg
     4141 cgtaacttac aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa
     4201 ccagtgtatt gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg
     4261 atggcagaac aaaatttgct cgggtgatcc cgctaatatt cacatgtcac gtacctttct
     4321 cgctcgcatg cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac
     4381 gcttagcgac tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagatcctac
     4441 tgcccaaaac ctgaaatatc atattaccgg tctggaaaac attcgcgtgc tggattgcag
     4501 cgtcaatttt tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct
     4561 ggatattcgt cgccacacaa tgtcgaaaac attcagtatt aagactacaa aatcacaaaa
     4621 tgaccaatgc accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt
     4681 tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa
     4741 cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt
     4801 ggtctacgaa aaaacctata cggctgaact ttcgtccatt gcaggtaccc ccatcgaggc
     4861 tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc
     4921 ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat
     4981 tgctgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa
     5041 taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc
     5101 tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga
     5161 tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc
     5221 tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca
     5281 ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca
     5341 gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct
     5401 ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag
     5461 atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg
     5521 ttctggtcac ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct
     5581 ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta
     5641 cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg
     5701 cagtgctcca gtctggtcag ctcatcacca tcgaactccc ggaatggctg cttgcgatgg
     5761 cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg
     5821 cactacgccc gctaccgcaa atcctgctgt cgatttttgc tctgttggct atttgtgcgg
     5881 gtatggcgtg ggggctgacc cggtttatgc atattgattt tatgactgcc tacctcgcca
     5941 ccagccccgg cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg
     6001 cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg ggacctgcca
     6061 ttgcacggtt tatttcaacc tatgcgccga agcggtcggc ttagtgctgg cagccagggc
     6121 accagtaaaa cgggcgagat gacagcgtgg ttttctcaat gatgctgcca caacgttcgc
     6181 acggttcgcc atctcgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct
     6241 catccacctg cccccgcgta gcgtaagaaa atcgaggaat ctccagtaac gcgtgtgcga
     6301 gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct
     6361 gccagaggat ctccacccgc aaataattgc caagtcctgc cagaaacgcc tgctcgagca
     6421 gtaatcctgc aaactgacgg ttacgaaagc gcggcgacaa tacccgttct ttcaccacct
     6481 ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg
     6541 tcagttgttc cggggtcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt
     6601 cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa
     6661 ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaattcg ttggaaaaat
     6721 gagttaacaa cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg
     6781 attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga
     6841 tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat
     6901 ccttttttca ttctttatta agcggggtta tgctgcgaca acaatcccct ttttggcaaa
     6961 tgtagctcgt aatcggcggg cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac
     7021 cgtttgcgcc gcgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac
     7081 catctccagc gtttgcgcca gcgcctgttg ttcgttttca atcaacgcgc ctggctgact
     7141 tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt
     7201 tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat
     7261 caatgccgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt
     7321 cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc
     7381 acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg
     7441 cggcaactgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc
     7501 aattgcaaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc
     7561 tgcatgaaat ccacaggcaa tattggcaga ggaaaccagc gttaaaagct ctgcgtcgct
     7621 ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacgcta
     7681 attgttcaaa ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact
     7741 ggacaaaatg aatcggctga ccgagcggaa tttgcgcaag atggtacata tcagcctcaa
     7801 tgatgcaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg
     7861 gctgcccgtt atgtggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcgc
     7921 gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg
     7981 agctaagctg ccagggcgaa cgccagaatg catcctgcga ggcacgatcg aactcatgat
     8041 attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg
     8101 cttccataaa atcacgcttc gctttgccaa tcgggagtcg gtcaccatcc ttcagtaaac
     8161 ggccttccag cccgccaatc cccactttga gatcggtgct gcatgacccc attaccggcg
     8221 gaacatcaat accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa
     8281 gcgttaaacg ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat
     8341 ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa
     8401 cagttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagta
     8461 ggttagcaat gcgtaacgcg ggcatatcca gtgcgccgca gtggctgata cccgactggc
     8521 gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca
     8581 gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg
     8641 ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc
     8701 cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg
     8761 aacgagtaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag
     8821 atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa
     8881 ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc
     8941 gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc
     9001 gctctcctcc caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt
     9061 acgcaaaatc accgtaatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc
     9121 caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc
     9181 cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga
     9241 tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg
     9301 tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac
     9361 ctgcagcata aaaatgcaac ccctgctcgc gggctgaatg aatggtctgt tcagaaactt
     9421 cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc
     9481 cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg
     9541 gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg
     9601 tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg
     9661 ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat
     9721 acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga
     9781 ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac
     9841 gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg
     9901 tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca
     9961 gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct
    10021 gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac
    10081 atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc
    10141 taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt
    10201 tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg
    10261 tggaattatt atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct
    10321 gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac
    10381 caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct
    10441 ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat
    10501 ggcggtgatg ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga
    10561 gatccatccg tcattcctct atctgtccct ggcgattatc gtttgtggct atggtctgtt
    10621 taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga
    10681 tggcggtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctatcgc
    10741 ctgtggttac gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg
    10801 tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg
    10861 cgttaataaa aaagtactgc gtgcaacaaa ctttctcctg cctaactggg gatggctgct
    10921 ggttctgctg gtggcaacgc ctgcgctgat taccgtactg ttctggaaag agtggtcggt
    10981 atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa
    11041 agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag
    11101 tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg
    11161 cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa
    11221 tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc
    11281 gggtaatcgt accgtgcgca tctgggggaa atttgctctt ggccttggcc tgatgagcgc
    11341 cggattctgc attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct
    11401 gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt
    11461 tgccatgtcg caaattacgc gcattgaaat tccgggcgtg accggcgtat taaccggcat
    11521 ctacatgctg ctttctggcg cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac
    11581 atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga
    11641 agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg
    11701 gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aataggccag
    11761 caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta
    11821 cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag
    11881 cttcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc
    11941 gcgcagctcc ggtaaccatc ggcggataaa ctcaccttcg cgatcaaatt tctcgccctg
    12001 agttatcgga ttgaaaatac gaaaatacgg cgcagcatca gtacccgtag aagccgccca
    12061 ctgccagcca ccgttattag ctgccaaatc accatcaatc agctgcgaca tgaaatatcg
    12121 ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg
    12181 tagccggtta tgcatccagc cagtgctgtt aagctgacgc atagcggcat caacaatcgg
    12241 gtatcccgtt ttgcctttct gccaggcctg taaatgtgcg ggattgcttt gccactgcac
    12301 acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag
    12361 atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc
    12421 cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc
    12481 cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg
    12541 ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc
    12601 ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg
    12661 cgcgggctct atcgatccgc tactacgaac ttttggcgca gcgacacact ccggcatccc
    12721 ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg
    12781 attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac
    12841 cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg
    12901 atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac
    12961 actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc
    13021 tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg
    13081 cgtcgcccac tggcgtggtg tagcgatata taacgccagc acgcgtgcag acgaattgcg
    13141 gcaggcggca gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag
    13201 atgggtaatc ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa
    13261 taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc
    13321 agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc cgccaactgc
    13381 tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacgttg
    13441 gtgtgatcgc gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg
    13501 ttgtaaaaat catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg
    13561 cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga
    13621 agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata
    13681 cggattaggg tgctgtcatc aaatcgttgc tgattcatta ctgctcctga acggtttaca
    13741 ggagtaagtg tagacgttga gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca
    13801 aatttacgaa agcctgctcc ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc
    13861 cctttctatt caaacgtgaa atcaataagc tcatcaggga catcgcaaac aatatgaaga
    13921 cgggtttctt ctctgccgtg actcttttca ctaattgcgt aactgtcatg cttgggatta
    13981 tttaattctt tcagcggaaa tttttcctcg aaggctttat ttagccgccc ctggtttcct
    14041 tttacagcga ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg
    14101 caacccatcg catcggttgt gatgattttt cctttaatat ccagcatgtt aagaagttct
    14161 gggatagctg tgatctcatt ggatttctta tccgtcttga tctgcccgat gaccagactg
    14221 tgcattgttg agaacgcact aatgacatga atcgctcccc tgcggcgact cttgtcataa
    14281 gagtgccgga gcgtttttcc atcaattgca atgacgtctt tatcatttga tgaatggcaa
    14341 tcacgcatcc agttaataaa gcactcgtga aattttgcag g
//
LOCUS       DAZFRN010000125        14248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFRN010000125 DAZFRN010000000
VERSION     DAZFRN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.127"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            222..1280
                     /gene="citC"
                     /locus_tag="ACHKUB_003682"
     CDS             222..1280
                     /gene="citC"
                     /locus_tag="ACHKUB_003682"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO6656644.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLVPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1295..1591
                     /gene="citD"
                     /locus_tag="ACHKUB_003683"
     CDS             1295..1591
                     /gene="citD"
                     /locus_tag="ACHKUB_003683"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO6656645.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1588..2496
                     /gene="citE"
                     /locus_tag="ACHKUB_003684"
     CDS             1588..2496
                     /gene="citE"
                     /locus_tag="ACHKUB_003684"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO6656646.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPTDALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2507..4039
                     /gene="citF"
                     /locus_tag="ACHKUB_003685"
     CDS             2507..4039
                     /gene="citF"
                     /locus_tag="ACHKUB_003685"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO6656647.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4043..4594
                     /gene="citX"
                     /locus_tag="ACHKUB_003686"
     CDS             4043..4594
                     /gene="citX"
                     /locus_tag="ACHKUB_003686"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO6656648.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4569..5447
                     /gene="citG"
                     /locus_tag="ACHKUB_003687"
     CDS             4569..5447
                     /gene="citG"
                     /locus_tag="ACHKUB_003687"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO6656649.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5498..6961
                     /gene="citT"
                     /locus_tag="ACHKUB_003688"
     CDS             5498..6961
                     /gene="citT"
                     /locus_tag="ACHKUB_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO6656650.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7075..7881
                     /gene="rna"
                     /locus_tag="ACHKUB_003689"
     CDS             7075..7881
                     /gene="rna"
                     /locus_tag="ACHKUB_003689"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO6656651.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8111..8521
                     /gene="rnk"
                     /locus_tag="ACHKUB_003690"
     CDS             8111..8521
                     /gene="rnk"
                     /locus_tag="ACHKUB_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO6656652.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8659..8748
                     /gene="yldA"
                     /locus_tag="ACHKUB_003691"
     CDS             8659..8748
                     /gene="yldA"
                     /locus_tag="ACHKUB_003691"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO6656653.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8752..9990)
                     /gene="ybdR"
                     /locus_tag="ACHKUB_003692"
     CDS             complement(8752..9990)
                     /gene="ybdR"
                     /locus_tag="ACHKUB_003692"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO6656654.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10211..10639
                     /gene="uspG"
                     /locus_tag="ACHKUB_003693"
     CDS             10211..10639
                     /gene="uspG"
                     /locus_tag="ACHKUB_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO6656655.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10761..12326)
                     /gene="ahpF"
                     /locus_tag="ACHKUB_003694"
     CDS             complement(10761..12326)
                     /gene="ahpF"
                     /locus_tag="ACHKUB_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO6656656.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12497..13060)
                     /gene="ahpC"
                     /locus_tag="ACHKUB_003695"
     CDS             complement(12497..13060)
                     /gene="ahpC"
                     /locus_tag="ACHKUB_003695"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO6656657.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13432..14178
                     /gene="dsbG"
                     /locus_tag="ACHKUB_003696"
     CDS             13432..14178
                     /gene="dsbG"
                     /locus_tag="ACHKUB_003696"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO6656658.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3400 a         3763 c         3581 g         3504 t
ORIGIN      
        1 aacttaaaac cattaaataa ataaaaaacc actaactcta tgtgaaataa atcaaaattt
       61 cacgcagaaa tactccttag gatgtatagc gaaaggagaa aaagatatac ctcgatcacc
      121 cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc aacattgaac aacattcgtt
      181 gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc aatgatattt
      241 tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg
      301 aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc cgcgatgaaa
      361 agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgcg atcagtgaat
      421 ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac ctcgcctatg
      481 agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg ctgttccgcc
      541 agtgcggttt ttccacgctg accagcgtac ccggcgtgat ggtgctgatg gaaaacagcg
      601 ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca gggaacaaga
      661 ttggctgcat tgtgatgaac gccaatccct tcacgaatgg tcaccgttat ctgattcaac
      721 aagctgcggc acagtgcgac tggttgcatc tgtttttagt caaagaagat tcttcacgct
      781 tcccctatga agaccggttg gatctggtgt taaaaggcac cgccgatatt ccacgcctga
      841 ctgtgcatcg cggctccgaa tacatcatct cccgcgctac gttcccttgc tacttcatta
      901 aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt ttccgtcagt
      961 acctcgttcc cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg
     1021 ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact atctccgcac
     1081 cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg atatccgctt
     1141 cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg ctggtccctg
     1201 cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac gcggcagctc
     1261 gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc
     1321 aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata cgcaggatat
     1381 cgacctgcaa atcaatagca gcgttgagaa acagtttggc gatgcaattc gcaccaccat
     1441 tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg atgacaaagg
     1501 cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg ccagcggtat
     1561 cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca acgtaaaact
     1621 cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt cagcaactcc
     1681 ttcatctacc cgactgatgc cctgatgttt gacctcgaag actccgtggc attacgtgaa
     1741 aaagacaccg cccgccgcat ggtttatcac gcgctgcaac atccgctgta tcgcgatatt
     1801 gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg gtgttaacga cctggaagcc
     1861 gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt
     1921 ctggatattg aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga acccggcagc
     1981 accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt ggaaatcgct
     2041 cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg
     2101 cgtacagaac gctccccgga aggaactgaa ctgctgttcg cacgctgttc cattttgcag
     2161 gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc taacaacgaa
     2221 gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg caaatcgctg
     2281 atcaacccgc gtcagattga tctgttgcac aacctctacg caccaaccca gaaagaagtg
     2341 gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg
     2401 gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg tctggtgctc
     2461 tctcgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga cgcagaaaat
     2521 tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcgctg aatgcgatct
     2581 tgccgctttc cagaactcgc caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa
     2641 actgtgcgcc aacctggaag aagcgattcg tcgctctggt ttacaggacg gcatgacggt
     2701 ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga tggacgtcat
     2761 cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc tccctgagtg attgccatgc
     2821 gccgttggta gaacacattc gccagggcgt ggttacccgc atttatacct ccggcctgcg
     2881 cggtccactg gcggaagaga tctcccgtgg tctgctggca gaaccggtgc aaatccactc
     2941 tcacggcggt cgcgtgcatc tggtacagag cggcgaactg aatatcgacg tggctttcct
     3001 cggcgtcccg tcctgcgatg aattcggtaa tgccaacggc tacaccggta aagcctgctg
     3061 cggctccctc ggctatgcaa tggttgatgc cgacaacgca aagcaggtcg tgatgcttac
     3121 cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc aggttgattt
     3181 gatcgtcaaa gttgaccgcg ttggcgatgc tgcaaaaatc ggcgctggcg cgacccgtat
     3241 gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga ttgtcaactc
     3301 aggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgcat cgctggcggt
     3361 aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact tcgcccttgg
     3421 cggtattacc gcgacgatgg ttgacctgca cgaaaaaggt ctgatccgca aactgctgga
     3481 tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca atcacatcga
     3541 aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc gcatcggttg atcgtctcga
     3601 cgtggtggta ctgagcgcgc tggaaattga cacccagttc aacgttaacg tgctgaccgg
     3661 ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc gataccgcga ttgcctctgc
     3721 gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt ccgactctgg tggataacgt
     3781 gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc acggtatcgc
     3841 agttaacccg gcacgtccgg aactggcaga acgtctgcag gaagcgggca ttaaagtggt
     3901 ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac agccgattga
     3961 attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt
     4021 gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc accatgcggt
     4081 gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac acgtctggct
     4141 caagcgccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc cgattaaaga
     4201 cagcgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg ccttagccgc
     4261 aaaacagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg ggccggaggg
     4321 catgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg agcttgaaca
     4381 tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag gcgaaattct
     4441 ctcccgccgc gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc aaagcgcagc
     4501 cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca tggaggcact
     4561 gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa
     4621 tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga
     4681 aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg ctggaagatt
     4741 tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa tttggtgcct
     4801 gtagtgcaga aatggcaccg gaagcggtac tccacggatt acgcccaatt ggtatggctt
     4861 gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaaggc agcatttttt
     4921 ctttagggct gctatgtgcg gcaattggcc gtttgcttca gctcaaccag ccggtaacgc
     4981 caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg tctgaccgat cgcgaactgc
     5041 gtaccaataa ttcacaactg acggcaggtc aacggttgta ccagcagctt ggccttaccg
     5101 gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat caaccacgcc ttgccgcatt
     5161 acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat accttgctcc
     5221 tactgatggc gatcaacggc gataccaacg ttgcttcgcg cggtggcgag gggggcctgc
     5281 gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga acccccgccg
     5341 atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc agtccaggcg
     5401 gcagtgccga cctgctgatc cttacctggt ttttagcaca gatttaatta tttaagcact
     5461 tgataaattt gaaaatatta attttcggag aacccgtatg tctttagcaa aagataatat
     5521 atggaaacta ttggccccac tggtggtgat gggtgtcatg tttcttatcc ctgtccccga
     5581 cggtatgcca ccacaggcat ggcattactt cgctgtgttt gtggcaatga ttgtcggcat
     5641 gatcctcgag ccaattccgg caacagcgat cagttttatt gcggttacta tttgcgttat
     5701 tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt ttaatgcgca
     5761 aaaacaggcg ctgaaatggg gtctggctgg tttttccagc accacggtat ggctggtatt
     5821 tggcgcattt atttttgcat tagggtatga agtttccggg ttaggtcgtc gcattgccct
     5881 tttcctggtg aaattcatgg gcaaacgcac gctgacgttg ggttatgcga ttgtcattat
     5941 cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg gtacggtttt
     6001 tccggtcatt aaaaacctgc cgccgttgtt taaatcattc cccaacgatc cgtccgcgcg
     6061 tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga gttcgtccat
     6121 gtttgtcacc ggtgcggcac caaacgtgct gggtctggag ttcgtcagca aaattgccgg
     6181 tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg ttatcttgct
     6241 tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac acagtgaaga
     6301 agtggcaacc tgggcgggtg atgaactaaa aaccatgggt gcgctgacac gcagagagtg
     6361 gacgctgatt ggccttgtat tgctcagctt aggtttgtgg gtatttggca gtgaagtcat
     6421 taatgctact gcggttggtc tgctggcagt ttcgctaatg ctggccctgc atgttgtgcc
     6481 gtggaaagac attacccgct ataacagcgc atggaacacg ctggtcaacc tggcaactct
     6541 ggttgtgatg gctaacggcc tgactcgttc tggttttatt gactggttcg ccggtaccat
     6601 gagtacacac ctggaaggat tctcaccaaa cgcaacggtg attgtactgg ttctggtgtt
     6661 ctactttgca cactacctgt ttgccagcct gtctgcgcac accgcaacca tgctgccggt
     6721 tattctggcc gtcggtaaag gtattccggg cgtaccaatg gaacaactgt gtatcctgct
     6781 ggtgctgtct atcggtatca tgggctgtct gacgccgtat gcaaccggtc ctggggtgat
     6841 tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg caatcttcgg
     6901 ggtgatttat atctctatgt tgctgttggt tggctggccg attctcgcca tgtggaacta
     6961 atacgttcgc caaacgctga ataaatcatc acgcccgcca ggttagccca cctggcgggc
     7021 ttttttatga tttaatagat agtattcagt gtatgtatga gttccacacc cattatgaaa
     7081 gcattctggc gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc ttctgccaac
     7141 gccgtagcgt tgcaggcaaa acagtatggc gactttgatc gctatgtcct ggccctctcc
     7201 tggcaaaccg gattttgcca gagtcaacac gatcgaaatc gtaacgaacg agatgaatgt
     7261 cgcctgcaaa ccgaaacgac caacaaagct gattttctga ccgtacatgg tctgtggcca
     7321 ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat gcgcttcggt
     7381 tgcgctactc gcccaatccc gaatctacca gaagcgcgcg ccagccggat gtgttcatcg
     7441 ccggaaaccg gattatcact ggaaacggcc gctaaactaa gtgaagtcat gccaggagct
     7501 ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg ctttggtttt
     7561 gatccggacg catacttcgg tacgatggta cgcctgaatc aagaaattaa agagagcgaa
     7621 gccggaaaat tccttgcgga taattacggt aaaacagtga gccgccgtga ctttgacgcc
     7681 gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac gtgccagggt
     7741 aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgccat caacgctccg
     7801 ctttctgcaa actcattctt gccacaacca cacccaggca actgtggcaa aacctttgtg
     7861 attgataaag tgggttatta atcatctgat ttacaaaaca cgctaattcg tcttccataa
     7921 accgtgacga ttaagaatta tacataatgc gctgcattga ggaattccct gaatgtgacg
     7981 caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa ccctcgccgc
     8041 ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagt accgacgaca
     8101 tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg aacgcatcga
     8161 tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt taaacgcaga
     8221 gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg tgacaatgaa
     8281 cagccgggtt aaattccgca atcttagcga tggcgaagtg cgtgtgcgca cgctggtgta
     8341 tccggcaaaa atgaccgata gcaatactca gctttccgtt atggctccgg taggtgccgc
     8401 actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg gcgttgcaac
     8461 ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact acctgcttta
     8521 atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca tcctctttac
     8581 gcaaaatcag caatttttcc tggttctcac tgttcacttt tgctgcaaaa ttattccgtt
     8641 tcgtttgtgg gaataaccat ggcagaagca ttttacatcc tgataggatt tctgattatg
     8701 gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc actcctgacg atcatattgt
     8761 tccccccggc atcgcattca ccagacctga aaccgccttc tgcgccgcct ctgcgctttg
     8821 tgcaccgggt accagaatca ccttacggca ctcctcttca cgtttttcga aaatctcata
     8881 tccccgggcg gcctcttcaa acggcatata gtgggtaaca atttcttctg gtttcagtaa
     8941 tcctttctca attaacggta ataattctcc cagccatgcg tgaacgtggg tctgtcccat
     9001 tttaaacgtc aaccctttat caaaggcgtc gccaaacagg aaaccgtgaa taaatccagc
     9061 gtagacgccc ggtacgctaa caatgccgcc acgcctgacc gccgcaatac actggcgcaa
     9121 cgctttaccg ctgctgccct ccagtttcag gttagtcagc accgtttccg tggtgctgcc
     9181 tttcgcttca aaaccgacgg cgtctattac tgcatccacg ccccggtgac ctgccgtttg
     9241 ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa ttaatcggga tcgcgccgta
     9301 gcggtcagcg gcgaaacgca agcggtaggg atgatgatca acaacaaaaa tctgttccgc
     9361 accgagcaac cgtgcacagg cgattgtcaa caatcccact ggaccggcac catagactgc
     9421 aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc tgccatgccg ttggcagaat
     9481 atcagaaagg aaaagggctt tatcatctga aagcaaaggc ggtactttaa acggccccac
     9541 attcccttta gggacgcgga catattccgc ctgtccacca ggaacgccac catacaggtg
     9601 actataacca aacaatgccg cgggagctgg tatctgtttt ttattgagcg cagcgccttt
     9661 acccgcattg gtattttcgc aggcggcata ttgctgcatt cgacagaaaa aacagtcgcc
     9721 acaagcaatg acaaacggaa ttaccacccg gtcgcctttt tgcaaatttt ttacgtcctt
     9781 tccggtttca acgacttccc ccataaattc atgaccaaaa atatcgccat gtttaacctg
     9841 gggtattttg cctcgataaa gatggaggtc agagccacag atcgccgttg ccgtaatacg
     9901 cagaataata tcatctgcct gttcaatgcc cggatcggga acattttcta cctgaacgtg
     9961 atgtgggccg tgataagtca atgctttcat gcaacctcca gagacagttc aaaaaagagt
    10021 cagtctttaa gggtagctat cacagcggga aaacgggcgg taccgcaggt tttgggagaa
    10081 ttcctggtgt tcagtgagaa tatattaatc acagaaaccg ttcgtaagga aaaagaacca
    10141 gacaaatatc agggataaat attataatca tggcgaatga tttgttcatg attaacaggg
    10201 agaaagggtt atgtataaga caatcattat gccagttgat gtatttgaaa tggaattgag
    10261 cgacaaagct gttcgccacg ctgaattcct cgcccaggat gacggagtta ttcatctact
    10321 tcacgtacta cccgggtcag ccagcctgag cctgcaccgt tttgccgctg atgtgcgtcg
    10381 ttttgaagag catctgcaac atgaagcaga agaacgtctg caaacgatgg tcagccactt
    10441 caccatcgat ccttcccgca ttaaacaaca tgtccgtttt ggtagcgtgc gggatgaagt
    10501 caatgagttg gcagaagaac tgggggctga tgttgtagtt attggttctc gcaacccatc
    10561 gatttcgacc catctgttag gttctaacgc ctcgagcgtg atccgccatg ccaatctgcc
    10621 ggtgctggtt gtgcgttaat tgccagaata aatatcccgc cctgcccgga gccatcccgg
    10681 cagggcattt ttaatgccat aaaaaagccg ccaggtttga ccccggcggc taagcaattg
    10741 caggtgaatc ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc
    10801 agagaggctt tggcaccttc gccagtggcg atgatgatct gcttgtacgg aaccgtcgta
    10861 cagtcacccg ctgcgaatac gcctttgacg ttggtttcgc atttcgcatc aatgataatc
    10921 tcacccatgc ggttacgttc gactgcgcct tccagccagt tggtgttcgg cagcagacca
    10981 atctggacga aaataccggc cagttcgatg ttgtgaatat cgccgctgac acgatctcga
    11041 tattccagac caacgacttt gctgccgtcg cctttcactt ccgtggtttg cgcattcaga
    11101 ataatgtcga cgtttttcag gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt
    11161 tctggcgcaa attcgagcag cgttacgtgc tcaacgatac ccgccaggtc aattgccgct
    11221 tccacgccgg agttaccgcc gccgataacc gctacgcgtt tgcctttaaa cagcgggccg
    11281 tcgcagtgcg ggcagtaggt cacgcctttg gtgcgatact gatcttcgcc aggaacgttc
    11341 atgttgcgcc attttgcacc ggtcgccacg ataatgctgc gtgctttcag taccgcgcca
    11401 gaagctgttt caatctgatg cagaccacct tcaactgctg ctgggatcag tttgctggcg
    11461 ctctggctgt cgatcacatc aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc
    11521 agcttctgcc cttcagtctt cggtacagaa atgtagtttt cgatatcaac ggtatcgagg
    11581 atctgaccac caaaacgttc gcccatcaga ccggtacgga tgcctttacg tgcggagtaa
    11641 attgctgcag ctgcacccgc cgggccggaa ccaacgatta atacgtcata agcatcacgc
    11701 ttgttcagct cttctgccgc acgtttttcc gcgcccgtat caattttggc aacgatttca
    11761 gtcaacgtca tgcggccctg accaaactct ttcccgttta cgaacactgc cggaacgccc
    11821 atcacgttgc gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg
    11881 atgcgcgggt tcagtacgct catcaggttc agcgcctgca ccacgtccgg gcagttatgg
    11941 caagagagcg agtaataggt ttcgaattca aaatcaccgt caatatggcg aatctgctcc
    12001 agcagagact gcgcttcttt cgacggatga ccaccggtcc atagcaacgc cagtaccagc
    12061 gaggtgaact cgtggcccag cggggagcct gcaaaacgtg gcccctggtt ggaacctggg
    12121 ttggtgatca ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt aaaggtgact
    12181 ttgtctgaca gttctgcgat ttcagccaac agttccttga tttctgccga tttagcgctg
    12241 tcatccagcg tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta agccttgagt
    12301 tgagttttca tatttgtgtc gagcatgttt atctcctggg ctttaaacat catcatgcaa
    12361 gcggcctctg cggctacctg gatgcagctt gcatcatggt gcggggaaga aaatcgggtg
    12421 ccgcagcacc cgaagaatta ggtgaatttc cgaagtattt cgcgccggag ttggtagcgc
    12481 gaaagacgaa ggaaatttag attttaccaa ccaggtccag agacggagcc agagttgctt
    12541 caccttcttt ccatttagcc gggcaaactt cacctgggtg agaagctacg tactgtgctg
    12601 ctttgatttt acgcagcagg tcagacgcgt cacggccaat gccttcagcg gtaacttcga
    12661 ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc agaccttcat
    12721 cttcacgcat gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg atcatcgcat
    12781 atttgatttt agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg aagtgagtat
    12841 cggtagatac tgcgtatacg tctacgccca gtttctgcag ttcttcgtag tggtcagcaa
    12901 cgtcacccag ttcggtcggg catacgaaag taaagtcagc cgggtagaag aagaagacgc
    12961 tccagcggcc ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt ttgaatgcct
    13021 ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat ctatacttcc tccgtgtttt
    13081 cgatgagatg taaggtaacc aatttttgcc gattcggcta atgcgtttcc attatcaaat
    13141 cgataagttt taccttacaa ccttcgtaag acaactttgt gaactttgca ccgccagcgg
    13201 cgataaaaag taaaatggcc ccctttccag gtgaccgtgt aatctgaact accctttggt
    13261 atcttcagcg cctgcctgca ggctgaggtg ttgcccttac aaatgcaaca acgacatgga
    13321 ttacaacacc ctcataaaca aagggcaatc acctgatcta agctcttacc tatgacagtg
    13381 ataggttatg ccttttactc gacttttgca ctgactgaaa aggacaaatt aatgttaaaa
    13441 aagatacttt tactggctct gcttcctgca atcgccttcg cagaggaact tccttcccca
    13501 gtaaaagcga ttgaaaaaca gggcattaca atcatcaaaa cgttcgatgc ccccggagga
    13561 atgaaaggtt atctcggaaa gtatcaggat atgggcgtca ccatctacct gactcccgat
    13621 ggtaagcacg ctatctctgg ttacatgtac aacgagaaag gtgaaaacct gagtaacaca
    13681 cttatcgaaa aagaaattta cgcaccagcc ggacgcgaaa tgtggcaacg gatggaacaa
    13741 tcccactggc tcctcgacgg taaaaaagat gcgccggtca ttgtctacgt gttcgccgat
    13801 ccgttctgcc catattgtaa acagttctgg cagcaggcgc gcccgtgggt agattctggc
    13861 aaagtgcaat taagaacatt gttggttggg gttatcaagc cagaaagccc ggcgacagca
    13921 gcggcaattc ttgcctccaa agatcccgca aaaacctggc aagaatatga atcctctggt
    13981 ggcaagctta agctaaacgt gcctgcaaac gtaagtacag aacaaatgaa agtgttaagc
    14041 gccaacgaga aactcatgga cgatctgggg gcaaatgtca caccagctat ctattacatg
    14101 agtaaggaaa atacgctgca acaggccgtg gggttgcccg atcagaaaac gcttaatatc
    14161 attatgggga ataaataaaa gataattcat ggatggttga aatacatccc tgcttttcaa
    14221 ccatcctaaa aaagcatgaa ttgctcgc
//
LOCUS       DAZFRN010000126        14213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFRN010000126 DAZFRN010000000
VERSION     DAZFRN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.128"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            254..553
                     /gene="yoaC"
                     /locus_tag="ACHKUB_003697"
     CDS             254..553
                     /gene="yoaC"
                     /locus_tag="ACHKUB_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO6656659.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTENMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(673..852)
                     /gene="yoaH"
                     /locus_tag="ACHKUB_003698"
     CDS             complement(673..852)
                     /gene="yoaH"
                     /locus_tag="ACHKUB_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO6656660.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            926..2287
                     /gene="pabB"
                     /locus_tag="ACHKUB_003699"
     CDS             926..2287
                     /gene="pabB"
                     /locus_tag="ACHKUB_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO6656661.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2291..2869
                     /gene="nudL"
                     /locus_tag="ACHKUB_003700"
     CDS             2291..2869
                     /gene="nudL"
                     /locus_tag="ACHKUB_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO6656662.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3053..4417
                     /gene="sdaA"
                     /locus_tag="ACHKUB_003701"
     CDS             3053..4417
                     /gene="sdaA"
                     /locus_tag="ACHKUB_003701"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6656663.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGNEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4548..6146
                     /gene="pdeD"
                     /locus_tag="ACHKUB_003702"
     CDS             4548..6146
                     /gene="pdeD"
                     /locus_tag="ACHKUB_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6656664.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6150..7706)
                     /gene="yoaE"
                     /locus_tag="ACHKUB_003703"
     CDS             complement(6150..7706)
                     /gene="yoaE"
                     /locus_tag="ACHKUB_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO6656665.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7694..7852)
                     /gene="yoaL"
                     /locus_tag="ACHKUB_003704"
     CDS             complement(7694..7852)
                     /gene="yoaL"
                     /locus_tag="ACHKUB_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO6656666.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8169..9140
                     /gene="manX"
                     /locus_tag="ACHKUB_003705"
     CDS             8169..9140
                     /gene="manX"
                     /locus_tag="ACHKUB_003705"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO6656667.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9203..10003
                     /gene="manY"
                     /locus_tag="ACHKUB_003706"
     CDS             9203..10003
                     /gene="manY"
                     /locus_tag="ACHKUB_003706"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO6656668.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10016..10867
                     /gene="manZ"
                     /locus_tag="ACHKUB_003707"
     CDS             10016..10867
                     /gene="manZ"
                     /locus_tag="ACHKUB_003707"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO6656669.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10922..11380
                     /gene="yobD"
                     /locus_tag="ACHKUB_003708"
     CDS             10922..11380
                     /gene="yobD"
                     /locus_tag="ACHKUB_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO6656670.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11809..12375
                     /gene="mntP"
                     /locus_tag="ACHKUB_003709"
     CDS             11809..12375
                     /gene="mntP"
                     /locus_tag="ACHKUB_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO6656671.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12372..13181)
                     /gene="rlmA"
                     /locus_tag="ACHKUB_003710"
     CDS             complement(12372..13181)
                     /gene="rlmA"
                     /locus_tag="ACHKUB_003710"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO6656672.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13347..13556)
                     /gene="cspE"
                     /locus_tag="ACHKUB_003711"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13347..13556)
                     /gene="cspE"
                     /locus_tag="ACHKUB_003711"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6656673.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13569..13712)
                     /gene="yobF"
                     /locus_tag="ACHKUB_003712"
     CDS             complement(13569..13712)
                     /gene="yobF"
                     /locus_tag="ACHKUB_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO6656674.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3547 a         3531 c         3507 g         3628 t
ORIGIN      
        1 catgccgttg tgttcgccct ctgactagca actctggttt tctttcttag ctgaataatc
       61 tggttatagc gtgaggactg accgttaaat ttctccatct tactggataa cacaccatga
      121 gctgctacct ttaaaacacc tgaatgtcaa caggttaact cgtgccatat cgttataatc
      181 cctttgatat tagatgcaaa ttaaggtcat atatagtctt atcgataaca tggttaattt
      241 taaggataag aatatgcctg ctgtaataga taaagccctg gatttcattg gtgccatgga
      301 tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcaca gcgaagggaa tctttaaata
      361 tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt acggcgcgag ctgaccagga
      421 aggctggaac cctgggttca cggaaaacat ggttggatgg gcaaaaaaaa tggagtctgg
      481 tgaacgcact gtaattaaaa atcctgaata cttttcaaca tatatgcagg aagaactaaa
      541 agcactggtc tgagcttaat ttatatcagc ataaatgggt caggacgctt ttaatcacat
      601 ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg cttctataac gaaatcgata
      661 cggattagtg tgttactcgt cttcatcctc aaaacgcgcc acgattcgct cgccggagtg
      721 gttggcacgc agctcttctg ccaccagcgc gattgcctgg ccgctgctca tcccttgtgc
      781 catcagttcc tggatccgct cgacagcttt ttgctgctgt tcatgggtga gtgaaggtaa
      841 acctgcaaac attgttaact cctgctaaat tgttggcgct aattatttca tgctacccgg
      901 cacatagcca gtagagtcag gactgatgaa gacgttatct cccgctgtga ttactttacc
      961 ctggcgtcag gacgccgctg aattttattt ctcccgctta agccacctgc cgtgggcgat
     1021 gcttttacac tccggctatg ccgatcatcc gtatagccgc tttgatattg tggtcgccga
     1081 tccgatttgc actttaacca ctttcggtaa agaaaccgtt gttagtgaaa gcgaaaaacg
     1141 cacaacgacc actgatgacc cgctacaggt gctccagcag gtgctggatc gcgcagacat
     1201 tcgcccaacg cataacgaag atttgccatt tcagggcggc gcactggggt tgtttggcta
     1261 cgatctgggc cgccgttttg agtcactgcc agaaattgcg gaacaagata tcgttctgcc
     1321 ggatatggca gtgggtatct acgattgggc gctcattgtc gaccaccagc gtcatacagt
     1381 ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc tggctggaaa gccagcaatt
     1441 ctcgccgcag gaagatttca cgctcacttc cgactggcaa tccaatatga cccgcgagca
     1501 gtacggcgaa aaatttcgcc aggtacagga atatctgcac agcggtgatt gctatcaggt
     1561 gaatctcgcc cagcgttttc atgcgaccta ttctggcgat gaatggcagg cattccttca
     1621 gcttaatcag gccaaccgcg cgccatttag cgctttttta cgtcttgaac agggtgcaat
     1681 tttaagcctt tcgccagagc ggtttattct ttgtgataat agtgaaatcc agacccgccc
     1741 gattaaaggc acgctaccac gcctgcccga tcctcaggaa gatagcaaac aagcagaaaa
     1801 actggcgaac tcagcgaaag atcgtgccga aaatctgatg attgtcgatt taatgcgtaa
     1861 tgatatcggt cgtgttgccg tagccggttc ggtaaaagta ccagagctgt tcgtggtgga
     1921 acccttccct gccgtgcatc atctggtcag cactataacg gcgcgactac cagaacagtt
     1981 acacgccagc gatctgctgc gcgcagcttt tcctggtggc tcaataaccg gggctccgaa
     2041 agtacgggct atggaaatta tcgacgaact ggaaccgcag cgacgtaatg cctggtgcgg
     2101 cagcattggc tatttgagct tttgcggcaa catggatacc agcattacta tccgcacgct
     2161 gactgccatt aacggacaaa tatactgctc tgcgggcggt ggaattgtcg ccgatagcca
     2221 ggaagaagcg gaatatcagg aaacttttga taaagttaat aagatattac gccaactgga
     2281 gaagtaagac gtggaatacc gtagcctgac gcttgatgat tttttatctc gctttcaact
     2341 tttgcgcccg caaattaacc gggaaaccct aaatcatcgt caggctgccg tgttaatccc
     2401 catcgtccgt cgaccgcaac cggggttgtt gctgactcag cgttcaattc atctgcgtaa
     2461 acacgctgga caagtggcat tccctggagg tgcagtcgat gacacggacg catcagttat
     2521 cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata ccgccttccg ccgttgaagt
     2581 tatcggcgtg ctgccgcccg tcgatagcgt aactggctat caggtaaccc cagtggtcgg
     2641 cattatcccg cccgatctgc cgtatcgcgc cagtgaagat gaagtctcgg cggtgtttga
     2701 aatgccgctc gcccaggcat tacatctggg tcgttatcac cctttagata tctaccgccg
     2761 tggcgattca catcgggtat ggctgtcctg gtacgaacag tattttgtat ggggaatgac
     2821 cgcaggcata attcgtgagc tggcgctgca aattggtgtg aaaccctgac tatacttatc
     2881 tttacatcta caaaacacta cttgagacaa tcatcgcaat attagttaaa tcgcggtttt
     2941 tgattagttt aattcatgtg aatagttaag ccagtcgccg cgttccctct tacactatgc
     3001 gctgttatta gttcgttact ggaagtccag tcaccttgtc aggagtatta tcgtgattag
     3061 tctattcgac atgtttaagg tggggattgg tccctcatct tcccataccg tagggcctat
     3121 gaaggcgggt aaacagttcg tcgatgatct ggtcgaaaaa ggcttactgg atagcgttac
     3181 tcgcgttgcc gtggacgttt atggttcact gtcgctgacg ggtaaaggcc accacaccga
     3241 tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc accgtggata tcgacagtat
     3301 tcccggtttt attcgcgacg tagaagagcg cgaacgtctg ctgctggcac agggacggca
     3361 tgaagtggat ttcccgcgcg acaacgggat gcgttttcat aacggcaacc tgccgctgca
     3421 tgaaaacggt atgcaaatcc acgcctataa cggcaatgaa gtcgtctaca gcaaaactta
     3481 ttattccatc ggcggcggtt ttatcgtcga tgaagaacac tttggtcagg atgctgccaa
     3541 cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa ctgctcgcgt actgtaatga
     3601 aaccggctat tcgctgtctg gtctcgctat gcagaacgaa ctggcgctgc acagcaagaa
     3661 agagatcgac gagtatttcg cgcatgtctg gcaaaccatg caggcatgta tcgatcgcgg
     3721 gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg ccacgtcgtg cgtctgccct
     3781 gcgccggatg ttagtttcca gcgataaact gtctaacgat ccgatgaatg tcattgactg
     3841 ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc gccggtggtc gtgtggtaac
     3901 tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg ctggcttact atgaccactt
     3961 tattgaatcg gtcagcccgg acatctatac ccgttacttt atggcagcgg gcgcgattgg
     4021 tgcattgtat aaaatgaacg cctctatttc cggtgcggaa gttggttgcc agggcgaagt
     4081 gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa ctgctgggcg gtagcccgga
     4141 acaggtttgc gtggcggcgg aaattggcat ggaacacaac cttggtttaa cctgcgaccc
     4201 ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat gccattgcct ctgtgaaggc
     4261 gattaacgcc gcacggatgg ctctgcgccg caccagtgca ccgcgcgtct cgctggataa
     4321 ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac gccaaatacc gcgaaacctc
     4381 acgcggtggt ctggcaatca aagtccagtg tgactaatac ttcttactcg cccatctgca
     4441 acggatgggc gaatttatac ccgctttctc gtctgctgta atattcccca ctacacttcc
     4501 actgttgcgt caggcgtttg tcgccatacg cttacagggt ggcccgcatg caaaaagcac
     4561 aacggatcat taaaacctat cgccgtaatc gaatgattgt ttgtacgatt tgcgcactcg
     4621 ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca gcgtaactta aatcaacaac
     4681 gggtagtaca attcgccaat cacgctgtag aggaattaga taaagtactg cttcccctac
     4741 aggcaggtag cgaagtcttg cttccgctga ttggtctgcc ctgctctgtc gcccatttgc
     4801 cattacgtaa acaggcggca aaactccaaa ctgtgcgatc cattggcctg gtgcaagacg
     4861 gcacactcta ttgctccagc atttttggtt atcgcaatgt gcccgtcgtg gacattctgg
     4921 ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat cgaccgtgcc ctgattaaag
     4981 gcagtccggt tttgattcaa tggacgcctg cagcgggcag tagcaatgct ggggtcatgg
     5041 agatgattaa catcgactta ctgacggcaa tgctccttga gccacaactg ccgcaaatca
     5101 gtagcgccag cctgacggtg gacaaacggc atttgctcta tggtaatggg ctggtagatt
     5161 cccttccgca acctgaagac aatgaaaact atcaggtttc ttcgcaacgc tttcctttta
     5221 ccattaacgt taatggtccg ggggctacgg cgctggcatg gcactatctt ccaacacaat
     5281 taccgctggc ggtgctgcta agtttactgg tgggctacat cgcctggctg gcgaccgctt
     5341 accggatgag cttttcccgc gaaatcaatc tgggcctggc gcaacatgag ttcgaattgt
     5401 tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat tggtgtagag attttgctgc
     5461 gctggaataa tccgcgtcag ggctggattt caccggatgt gtttattcct atcgcggaag
     5521 aacatcattt aattgtgcca ctgacccgct atgtgatggc agaaaccatt cgtcagcgcc
     5581 atgttttccc gatgagtagt cagtttcatg ttggcattaa cgtcgcaccc agccattttc
     5641 gccgtggtgt gctgataaaa gatctcaatc agtactggtt tagcgctcac ccgattcagc
     5701 aactgatcct cgaaatcacc gaacgcgatg ccttactgga tgttgattat cggattgccc
     5761 gcgagctaca tcgtaaaaac gtcaaactgg cgattgatga cttcggcacc ggcaacagtt
     5821 cattttcctg gcttgaaaca ttacgtcctg acgtgctgaa aattgataag tcatttaccg
     5881 cagctatagg ttctgacgcg gttaactcga cggtgaccga tatcatcatc gcgctggggc
     5941 aaagactgaa tattgaactg gtggcggagg gcgtggaaac gcaagaacag gcgaagtatt
     6001 tgcgccgtca tggcgtgcat attttgcaag ggtatttgta cgcacagccg atgccgctac
     6061 gtgattttcc caaatggctg gcgggcagcc aaccgccgcc cgcccggcat aatggacata
     6121 tcacgcccat tatgccgtta cgttaatgct tactcatctt catcgtgcgc cggttgctct
     6181 ttaacaatgc gaaccagatc aacacgataa tcattggctt caatgatggt gatatgcagt
     6241 ggccctacat caatcacatc gcccacacgg ggaatgtgac catttgccga gatcacgagg
     6301 cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct caacatcaag cgcctgctgc
     6361 aaggcatgca aatctgtacc gccttttacc agccagccgt caccatcagt aatgatttcc
     6421 ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt ccagcacatc cagcggcgtg
     6481 accagacctt gtaccacacc aaactcgttg gtcacgataa caaagctccc gcgagcacga
     6541 cgcagcacgc ccaacaggtt gatcggatcg agggtttccg ggacgataat cgccggagac
     6601 gccgaagcaa tcgccgccac atcaacgccc tcttccagcg ccaccagcag ttctttagca
     6661 cgtacaatac cgatgatttc atccagttca ccgcgacata ccgggaacag actgtgcggt
     6721 gaagagagca gttgctcgcg gatttcatcg accccgagat tagcgtcaac ccagcttatt
     6781 tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg ccagcgtcag tacgccgtta
     6841 atcatgtaac gttcttcttc ggcaaatgca ccttccggga tcggcatcgg catcgggtta
     6901 tcggcatcgt gctgaacatt ggcctgacgt ttcccgccca tcaaacgcag gatggcatcg
     6961 gcagtacgcg ctcgcagcgg caaagtcgac tggtggcgaa taaagttgcg acgcgcaatc
     7021 tggttaaaca cttcgatgat gatcgagaag ccaatcgcgg catacaggta acctttcgga
     7081 atgtggaaac cgaaaccttc tgccaccaga ctcagaccaa tcattaacag gaagctcaga
     7141 cagagcacca ccaccgtggg gtgctggtta acgaatcgcg tcagcggttt ggatgccagc
     7201 aacataaccg ccatcgcaat cactaccgcc gccatcatca ccggcagatg gttaaccatc
     7261 cctactgcag taattaccgc atccaacgag aagacggcgt caaggatgac aatctgtgtg
     7321 acgaccaccc agaaactggc gtagccttta ccgtggccgg aatcatgatc gcggttttcc
     7381 agccgttcat gcagttcggt tgttgctttg aacagcaaga atatcccccc gaacaacata
     7441 atcaggtcgc gtccggagaa ggagaaatcc atgacggtaa atagcggttt ggtcagcgtg
     7501 accatccatg aaatcagcga cagcagcccc agacgcataa tcagcgccag tgataacccc
     7561 agcaaacgcg ctttatcgcg ttgttttggc ggcagtttgt cagcaagaat ggcgatgaag
     7621 accaggttat cgatacccag cacaatttcg agaacaacaa gcgtgagtag ccccgcccaa
     7681 attgaggggt ccattaagaa ttccatgaca agctcctgct taaggaatag ctattcgacg
     7741 ccagaaataa tgcaggcgta acgacaaaat gcaaacgaaa ggtgcggcat agagagccag
     7801 aaaggtaggc gttaaaaggc ctgatgctga aatgacgtcg gtgacgatcc atactgcggg
     7861 ctactgccct atactccatg gttgttaaac gggagttaaa catatcagag acgcctctga
     7921 tttggcaaag atttaccttc ctttgcaaac gaatgtgaca aggatatttt acctttcgaa
     7981 atttctgcta atcgaaagtt aaattacgga tcttcatcac ataaaataat tttttcgata
     8041 tctaaaataa atcgcgaaac gcaggggttt ttggttgtag cccttatctg aatcgattcg
     8101 attgtggacg acgattcaaa aatacatctg gcacgttgag gtgttaacga taataaagga
     8161 ggtagcaagt gaccattgct attgttatag gcacacatgg ttgggctgca gagcagttgc
     8221 ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt cggctggatc gatttcgttc
     8281 caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc tcagttggca aaactcgaca
     8341 ccactaaagg cgtgctgttt ctcgttgata catggggagg cagcccgttc aatgctgcca
     8401 gccgcattgt cgtcgacaaa gagcattatg aagtcattgc aggcgttaac attccaatgc
     8461 tcgtggaaac gttaatggcc cgtgatgatg acccaagctt tgatgaactg gtggcactgg
     8521 cagtagaaac aggccgtgaa ggcgtgaaag ctctgaaagc caaaccggtt gaaaaagccg
     8581 cgccagcacc cgctgccgca gcaccaaaag cggctccaac tccggcaaaa ccaatggggc
     8641 caaacgacta catggttatt ggccttgcgc gtatcgacga ccgtctgatt cacggtcagg
     8701 tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt agtgatgaag
     8761 tggctgcgga taccgttcgt aagacactgc tcacccaggt tgcacctccg ggcgtaacag
     8821 cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa tatgctggcg
     8881 aacgcgtaat gctgttattt accaacccaa cagatgtaga gcgtctcgtt gaaggcggcg
     8941 tgaaaatcac ctctgttaac gtcggtggta tggcattccg tcagggtaaa acccaggtga
     9001 ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt caagaaactg aatgcgcgcg
     9061 gtattgagct ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg atggatctga
     9121 tcagcaaaat cgataagtaa cgtattgtgt tgattatcac tcagttttca cacttaagtc
     9181 ttacgtaaac aggagaagta caatggagat taccactctt caaattgtgc tggtatttat
     9241 cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa tttcagtttc accgtccgct
     9301 aatcgcgtgt accctggtgg gtatcgttct tggggatatg aaaaccggta ttattatcgg
     9361 tggtacgctg gaaatgatcg cgctgggctg gatgaacatc ggtgctgcag ttgcgcctga
     9421 cgccgctctg gcttctatca tttctaccat tctggttatc gcaggtcatc agagcattgg
     9481 tgcaggtatc gcactggcaa tccctctggc cgctgcgggc caggtactga ccatcatcgt
     9541 tcgtactatt accgttgctt tccagcacgc tgcggataag gctgctgata acggcaacct
     9601 gacagcgatt tcctggatcc acgtttcttc tctgttcctg caagcaatgc gtgtggcaat
     9661 tcctgccgtc atcgtggcgc tgtctgttgg taccagcgaa gtacagaaca tgctgaatgc
     9721 gattccggaa gtggtgacca atggtctgaa tatcgccggt ggcatgatcg tggtggttgg
     9781 ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg atgccgttct tctacctcgg
     9841 cttcgtaacc gcagcattca ccaactttaa cctggttgct ctgggtgtga ttggtactgt
     9901 tatggcagtg ctctacatcc aacttagccc gaaatacaac cgcgtagccg gtgcgcctgc
     9961 tcaggcagct ggtaacaacg atctcgataa cgaactggac taacaggtga gcgaaatggt
    10021 tgatacaact caaactacca ccgagaaaaa actcactcaa agtgatattc gtggcgtctt
    10081 cctgcgttct aacctcttcc agggttcatg gaacttcgaa cgtatgcagg cactgggttt
    10141 ctgcttctct atggtaccgg caattcgtcg cctctaccct gagaacaacg aagctcgtaa
    10201 acaagctatt cgccgtcacc tggagttctt taacacccag ccgttcgtgg ctgcgccgat
    10261 tctcggcgta accctggcgc tggaagaaca gcgtgctaat ggcgcagaga tcgatgacgg
    10321 tgctatcaac ggtatcaaag tcggtttgat ggggccgctg gctggtgtag gcgacccgat
    10381 cttctgggga accgtacgtc cggtatttgc agcactgggt gccggtatcg cgatgagcgg
    10441 cagcctgtta ggtccgctgc tgttcttcat cctgtttaac ctggtgcgtc tggcaacccg
    10501 ttactacggc gtagcgtatg gttactccaa aggtatcgat atcgttaaag atatgggtgg
    10561 tggcttcctg caaaaactga cggaaggggc gtctatcctc ggcctgtttg tcatgggggc
    10621 attggttaac aagtggacac atgtcaacat cccgctggtt gtctctcgca ttactgacca
    10681 gacgggcaaa gaacacgtta ctactgtcca gactattctg gaccagttaa tgccaggcct
    10741 ggtaccactg ctgctgacct ttgcttgtat gtggctactg cgcaaaaaag ttaacccgct
    10801 gtggatcatc gttggcttct tcgtcatcgg tatcgctggt tacgcttgcg gcctgctggg
    10861 actgtaagac tgttgtacac taccggggcc ttttggcccc gtttttttat ctggaggatt
    10921 aatgacaatc acggacctgg tactgattct tttcatcgcc gcactcctgg ccttcgcgat
    10981 ctacgatcag ttcatcatgc cccgccgtaa cggccccacc ctgctggcaa ttcctttgct
    11041 ccggcgtggt cgcatcgata gcgttatctt cgtcggattg attgtcattc ttatctataa
    11101 caacgtcacg aatcatggtg cgttaataac gacatggtta ttaagcgcac tggctctgat
    11161 gggtttttat atattctgga tccgcgttcc gaagatcatc tttaaacaaa aaggtttttt
    11221 cttcgccaat gtctggattg aatatagccg aatcaaagcg atgaacttgt cggaagatgg
    11281 cgtgttggtg atgcaattag aacagcgtcg gctgttaatc cgcgttcgaa atatcgacga
    11341 tctggaaaaa atttataagc ttctcgtttc aactcaataa gttatgaatt tagccaaagc
    11401 tatgtttagt gtatttttaa taatcagaca tagcttaggc tatattacct cttcccttat
    11461 ttgttattta ttttaacgtt tcattgatat ataaatctaa atgtaaatcg ttatcaataa
    11521 agcaatgaaa taatatattc caacagttgt tttatattct caaaatatgt taaggttgcg
    11581 ccctcatttg gggagtagcc gatttccaga ttccggaaat gtacgtgtca acatactcgt
    11641 tgcaaaacgt ggcacgtacg gactgaatac tttcagtcag gcgagaccat atgcacatca
    11701 atcgctatgc ctgcatgaac gcgaaaacca tcgcggcttg ttgttcatgt atgcatgttt
    11761 gctgggggcg atgatgtgtt ttatggatac cccggtcagg acgttgtcat gaatatcact
    11821 gctactgttc ttcttgcgtt tggtatgtcg atggatgcat ttgctgcatc aatcggtaaa
    11881 ggtgccaccc tccataaacc gaaattttct gaagcattgc gaaccggcct tatttttggt
    11941 gccgtcgaaa ccctgacgcc gctgatcggc tggggaatgg gcatgttagc cagccggttt
    12001 gtccttgaat ggaatcactg gattgcgttt gtgctgctga tattcctcgg cgggcgaatg
    12061 attattgagg gttttcgtgg cgcagatgat gaagatgaag agccgcgccg tcggcacggt
    12121 ttctggctac tggtaaccac cgcgattgcc accagcctgg atgccatggc tgtgggtgtt
    12181 ggtcttgctt tcctgcaggt caacattatc gcgaccgcat tggccattgg ttgtgcaacc
    12241 ttgattatgt cgacattagg gatgatggtt ggtcgcttta tcggctcaat tattgggaaa
    12301 aaagcggaaa ttctcggcgg gctggtactg atcggcatcg gcgtccagat cctctggacg
    12361 cacttccacg gttaataaga acgctgccag aggtgaatat taaagtccgt ctggcagtcg
    12421 aacacttctt ttgctgccag tgtttgccag acttctggct tcgcacgcca ggcaaacggc
    12481 gtcatctgca ataatgcgac ggcttcatca ccgcgaagac gcatcggata acacaactca
    12541 tcactctgct gtaatgtaaa accttccagt tgttctgcat gaggtgcatg aagatgtact
    12601 tcattgtaaa tcagcccctt cagctccatc aaatgtcgcg gtcccggcgt ggcagtaatg
    12661 acccagccgc cgggcttcac tactcgtgct aattcttctg ctttacacgg cgcgtaaata
    12721 cgtattatgg cgtccatact ggtatcggaa aacggcaaac ggtggctgga agcgacacaa
    12781 agagtgacct gcggatagcg tttcgccgcc gcttttatcg ctaccttcga gacatccagg
    12841 ccaaacgtgg tgatttcggg caacgcatcg gcaaatgcgt gtgtgtaata cccttcacca
    12901 cagccaatat ccagcaccgc cgtggccttt tcatcaagcc gttcccgcag ttgggcgaca
    12961 attgcatcac gcagcggctg ataatgtccg gcatctaaga atgcgcggcg tgcttgcatc
    13021 atttccgcgc tgtcgcccgg atcacgagac cgtttatgct gaacgggcag cagattgaca
    13081 tacccttctt tcgccatatc aaactgatgt cgctggggac agatatagct gtttttttca
    13141 cgcgaaagag gctgatggca aagtggacag gaaaacgaca tgacaactcc ggcaggatac
    13201 taaaggccgc aagtgtaacg cgaatcacgc ggcgggcaaa tatccgaacg ccaggagcga
    13261 agataaagca tagtaaaaag cctcgcattc gacgaggctt tatagcgaga ttgaagcgta
    13321 ttcacacttc agatcagtgg attcgatcag atagctgtta cgttaacagc tgccggacct
    13381 ttctggccgt cctgaatttc gaactcaacg ttctgacctt cagccagagt tttgaagcca
    13441 ttaccctgga tagcggagaa gtgtacgaac acgtctttgc tgccatcagc cggagtaatg
    13501 aagccaaaac ctttagactc gttgaaccac ttaacctgac ctttaatctt tgccatttga
    13561 aaaattcctt agattgtttt cttcgcccgc aggcataaca tagataaaac tgacacatta
    13621 ctgcatgagg caccaatata aggctcggca gagaagcggt attcaacgtc aacgtgttta
    13681 ctcaggactt ctttactgaa aatgccacac ataaacagaa ctgtacctcg tttaacccga
    13741 aatctgttat cacatacaac gttaattatg gcaagccatt tttaaacatg tctcgatcag
    13801 acgcacaaat ctcgatacgt cttccacttt ttttgcacac ttatgcaacg gaatacgcgc
    13861 cgatatatca ttgtgcttaa ccttgccagt tcaggcagat acttaaactg gcgtagtttc
    13921 taacatagtt caataagttc agttgttcca ggccagtggg agaagttatt acatagtgcg
    13981 tgcaatatca cattttctgc tatgcaatga ataaaaagtt atatcacttt ttctcataaa
    14041 acagtcagtt aacggctatc aattacccta aagagaagtc aatccccaaa gggattgtaa
    14101 atttaaaata agaaaaattg atgagtgagc aaaaaaaaat caagagagaa acgtttcgct
    14161 aataaataat accacgctta atcacaaatc cgatgtaaac atcctaataa att
//
LOCUS       DAZFRN010000127        14017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFRN010000127 DAZFRN010000000
VERSION     DAZFRN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.129"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(51..347)
                     /gene="yaaX"
                     /locus_tag="ACHKUB_003713"
     CDS             complement(51..347)
                     /gene="yaaX"
                     /locus_tag="ACHKUB_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO6656675.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(561..1847)
                     /gene="thrC"
                     /locus_tag="ACHKUB_003714"
     CDS             complement(561..1847)
                     /gene="thrC"
                     /locus_tag="ACHKUB_003714"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO6656676.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="ACHKUB_003715"
     CDS             complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="ACHKUB_003715"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO6656677.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="ACHKUB_003716"
     CDS             complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="ACHKUB_003716"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO6656678.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5270..5389)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="ACHKUB_003717"
     CDS             complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="ACHKUB_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO6656679.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5604..6290)
                     /gene="yjtD"
                     /locus_tag="ACHKUB_003718"
     CDS             complement(5604..6290)
                     /gene="yjtD"
                     /locus_tag="ACHKUB_003718"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6656680.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6690..6830)
                     /gene="yjjY"
                     /locus_tag="ACHKUB_003719"
     CDS             complement(6690..6830)
                     /gene="yjjY"
                     /locus_tag="ACHKUB_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO6656681.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6926..7642
                     /gene="arcA"
                     /locus_tag="ACHKUB_003720"
     CDS             6926..7642
                     /gene="arcA"
                     /locus_tag="ACHKUB_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO6656682.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7702..9054)
                     /gene="creD"
                     /locus_tag="ACHKUB_003721"
     CDS             complement(7702..9054)
                     /gene="creD"
                     /locus_tag="ACHKUB_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO6656683.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9112..10536)
                     /gene="creC"
                     /locus_tag="ACHKUB_003722"
     CDS             complement(9112..10536)
                     /gene="creC"
                     /locus_tag="ACHKUB_003722"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO6656684.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10536..11225)
                     /gene="creB"
                     /locus_tag="ACHKUB_003723"
     CDS             complement(10536..11225)
                     /gene="creB"
                     /locus_tag="ACHKUB_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO6656685.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11238..11711)
                     /gene="creA"
                     /locus_tag="ACHKUB_003724"
     CDS             complement(11238..11711)
                     /gene="creA"
                     /locus_tag="ACHKUB_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO6656686.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11922..12791
                     /gene="robA"
                     /locus_tag="ACHKUB_003725"
     CDS             11922..12791
                     /gene="robA"
                     /locus_tag="ACHKUB_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO6656687.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12788..13435)
                     /gene="gpmB"
                     /locus_tag="ACHKUB_003726"
     CDS             complement(12788..13435)
                     /gene="gpmB"
                     /locus_tag="ACHKUB_003726"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO6656688.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13487..13999
                     /gene="yjjX"
                     /locus_tag="ACHKUB_003727"
     CDS             13487..13999
                     /gene="yjjX"
                     /locus_tag="ACHKUB_003727"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO6656689.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
BASE COUNT         3432 a         3822 c         3388 g         3375 t
ORIGIN      
        1 cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
       61 tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg gtggcgcggc
      121 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
      181 cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc acgatcgcct
      241 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc catgggagcg
      301 accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt
      361 tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga cggttgttat
      421 tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct ttttccctgt
      481 cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg
      541 attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagcaaaa
      601 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
      661 agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg
      721 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
      781 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
      841 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
      901 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
      961 gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
     1021 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgagtgactt cgccagcaga
     1081 cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgct
     1141 tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
     1201 atgttaatcg agttagctga gtttaacccc agcgccactt tcagttcttc atcatcaaac
     1261 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
     1321 ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
     1381 aggataacca ctttcacatt cggtaaaccg tagaaagcat gagccactgc cgctccggta
     1441 tcaccggagg tcgcggtcag aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt
     1501 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
     1561 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
     1621 acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa taaacgccga gaggatcttc
     1681 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
     1741 ggcaggtcat gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
     1801 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
     1861 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
     1921 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg
     1981 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
     2041 tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct
     2101 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagccagcc
     2161 agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagccctg
     2221 gcttctgccg tcgagacttt aatccccgga tacgccagca cccacagcca ctcatcaaac
     2281 cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat accaccaaga
     2341 aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga ttcgtccttc cagctcgccc
     2401 atcaaagcca gcaaacgagt gtcattaagc ggcttgccac agtgttcatt catcgccatc
     2461 agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgattgg catattcttt
     2521 tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac
     2581 tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg tccgaggttg
     2641 ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa tgcaccatca
     2701 acaggtgtca ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc
     2761 ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc
     2821 ggcaaagaca ccggcagctg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg
     2881 cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt tcactttgaa
     2941 cagcggatca ttaccatcca cttcggcaat cttcacgcgg caggcaccat cttcatcaat
     3001 attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa
     3061 gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct cagcgttaaa
     3121 ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc
     3181 acgagcgaga atcaatagct tacgcgctac atccatacca gaaagatcat ctcgcggatc
     3241 cggttcggta taacccattt cccgcgccag agtagtcgcc tcggagaaac tcatgccttc
     3301 gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcatcaa
     3361 ttcatcacca gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac
     3421 gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcatgacgca acagatggta
     3481 gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg
     3541 caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg
     3601 gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt
     3661 ggcttgcgcc agttcttcct gccagttttc cagatttagg ccatgcacat tggtgagcag
     3721 tgccttcgag ttggcaacac cgcagacacg taagtcgata tgtttattct tcagccagct
     3781 ttgttgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa
     3841 cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt
     3901 cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatggc
     3961 gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa
     4021 ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccagcgg
     4081 ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagctcg
     4141 cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat
     4201 cagcaccacg gaaatacggg cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac
     4261 cattcctttc atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat
     4321 gcccttgacc ggtaattcgt cttcatcacg actggcacca atgagcgtac ctggtgcttg
     4381 aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt
     4441 gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat
     4501 cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac
     4561 gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta
     4621 gtcggaaccg ttgcgtccaa gtaccaccag ttcgcctttt tcattaccgg cggtgaaacc
     4681 tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc gggtggactc
     4741 agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt cgaccggatc
     4801 gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat
     4861 tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact
     4921 aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag ttttcaattg
     4981 cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg
     5041 ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc
     5101 caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct gcccctgcct
     5161 ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg ccactgatgt
     5221 accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc
     5281 ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta
     5341 cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg
     5401 tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac
     5461 ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctatcag
     5521 acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg
     5581 aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa
     5641 cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgttg taaccagtcg
     5701 accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt
     5761 aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat
     5821 gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc
     5881 gccaccatcg gcacaccagt aagaacgtca gccaacgcta actcttcgtt agtcagcccg
     5941 gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac
     6001 agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg
     6061 gtggcgacag tgaaatcgac atcgtgtaac gattcaacca gtgtcgggaa aactttaata
     6121 ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc cagatgtgcc
     6181 tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc
     6241 ccaatatttt ctgctctggc gggtgcgacc agaataatcg ttatacgcat attgccactc
     6301 ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc ggtgcgaatt
     6361 tacaaattct taacgtaagt cgcagaaaaa gccctttact tagcttaaaa aaggctaaac
     6421 tatttcctga ctgtactaac ggttgaattg ttaaaaaatg ccacatatcc ttctgtttac
     6481 ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt tattagttgt
     6541 atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt agatacgatg
     6601 atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg aagcgtagtt
     6661 ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt taggatgaca
     6721 gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct aatgatgttg
     6781 ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat gttacgccga
     6841 tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc ctgtttcgat
     6901 ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg ttgaagacga
     6961 gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg atgttttcga
     7021 agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca acctggtgat
     7081 catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca
     7141 ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata aaattctcgg
     7201 cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg aactgacgat
     7261 tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg aagaacgtcg
     7321 tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc gttcgttgat
     7381 cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt
     7441 ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg
     7501 tgagctgaaa ccacacgacc gtactgtaga cgtgacgatc cgccgtattc gtaaacattt
     7561 cgaatctacg ccggatacgc cggaaatcat cgccaccatc cacggtgaag gttatcgctt
     7621 ctgtggtgat ctggaagatt aatcggcttt accaccgtca aaaaaaacgg cgctttttag
     7681 cgccgttttt atttttcaac cttatttcca gatacgtaat tgatcgtcca ttgtaacttc
     7741 tttactggct ttcattttcg gcagtgaaaa cgcataccag tcgatattac gagtcacaaa
     7801 catcatgccg gccagcgcca ccaccagcac actggttccc aacaacagcg cgctatcggc
     7861 agagttgagc agtccccaca tcacaccatc cagcaacaac agcgcgaggg taaacaacat
     7921 gctgttgcgc cagcctttca ataccgcctg caaataaata ccgttcatta acgccccaat
     7981 cagactggcg attatccatg ccacggtaaa accgatatgt tcagaaagcg ccagcaagag
     8041 caaataaaac attaccaatg aaagccccac cagcaaatat tgcattgggt gtaaacgttg
     8101 cgcggtgagc gtttcaaaaa caaagaacgc cataaaagtc agtgcaatca gcagaatggc
     8161 gtacttagtc gcccggtccg ttaattggta ttgatcggct ggcgtcgtca ctgcgacgct
     8221 aaacgcgggg aagttttccc agccggtatc attgcctgaa gcaaaacgct caccgagatt
     8281 attagcaaac cagctgcttt gccattgcgc ctgaaaacct gactcgctaa cttcccgttt
     8341 ggctggcaaa aaatcaccta aaaaactggg atgcggccag ttgctggtta aggtcatttc
     8401 gctattacgc ccggcaggca ccacagaaag atcgccggta ccgcttaaat tcagggccat
     8461 attcagcttc aggttctgct tccgccagtc cccttcgggt aaagggatat gcacgccctg
     8521 cccgccttgc tctaacccgg taccgggttc aatggtcagt gccgttccgt taacttcagg
     8581 cgctttcacc acaccaatac cacgcgcatc cccgacgcta atcacaataa agggcttgcc
     8641 taagatgata tttggcgcat tgagctcgct aagacgcgaa acatcgaaat cggcttttag
     8701 cgttaaatca ctgtgccaga cctgaccggt ataaatccct atcttgcgtt cttccacgtt
     8761 ctgattgcca tcaaccatca atgactcagg taaccaaaaa tggataaaac ttcgtttccg
     8821 ctccacggtt ttatcctctt cctgcaccgt ataaagctcg gtcacaggaa tagcgataag
     8881 cggcccaacg agtttttgcg gcccgctggt actttggcga atcgcatctt ccacatcgct
     8941 acggtaatcg gcacgttcga caatcacctg ccgaatcagc attatcggaa tcaacaacag
     9001 caatactgca ccaaacaggg tggtcatttt ccagaacagg ggggatttca acatagtctt
     9061 ctcctttgca atggcagcag gatacgaagg ctatgtggga agaatttgaa gctatgtgaa
     9121 gtgacggtga agtcgaagcg aggccagcac gccaccttcc tgcacgttgc gcagcgtgac
     9181 ttcgccgtta aacaaacggg cgacctcact gacgaacgcc aaccccagac cgctgctttt
     9241 ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc
     9301 aggaatacca ctaccggtat ccagcacctt aagcgtgacg tgttcctgat ccacttcggc
     9361 gcttagcgtt atacgaccgc tctcgggggt aaaatcgatg gcgttatcca gtaaattccc
     9421 cagcgcctga tccagtaacg ccggttcagc agcaacatta acctcggtgg gcgtaacatg
     9481 tagagtgatg tttttttctg ccaactgcac ggtgcgcgct tcgctgacgc ggcggaataa
     9541 tgccgccaca tcaacagcag tcaaaacgac ttcctgacga ttctccagtc ttgcctggcg
     9601 tagtaacgtt tccaccagtg cctgcattcg cgcattttgc gtcagaatgt tgtcggtaaa
     9661 acgagccacc acttccggtg gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc
     9721 cgccagtggg ctttttagct catgggttaa cgcataaaca tactgctcaa tatagttttt
     9781 cccttccagc ttcacgcgca tactttccag cgcctgcgca agcttacgca actcgctact
     9841 gccgagttca ggaagaggaa cgggcttatt gtcagtgacg gaatcggcat agcgagtgag
     9901 ccgggcaatg gagcggttga tccaccaaac catgcctgcg ccaatcacca gtgcaatccc
     9961 cagcaaaatg gcgctggccc ataaaattcg ccgctcgcta cgcttaatga ccggagccat
    10021 cgccgcgttc ggtttgccta cgctcaaaac gccaataagc cgcgagccgt ccataatcgg
    10081 cgcggcaaca tacatcaccg aactttcggg atcggcagga ttttgcaacg tgctgcgcgc
    10141 accatactga ccacgcaacg ttagccagac gtcattccag cgcgaataat cctgcccaac
    10201 ggctttattt gccgaatcga acaatacttt accctgcgaa tcggtcatat agacatgata
    10261 ttcattgcgc actttgttaa tgccaccgat attggcgcga aacgggcgat gttgtagctg
    10321 attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc
    10381 cagctccgcc agcaacgttg cggtatcgat taacgtcccc tccgttgctc ttcgcacgcc
    10441 cggtttaact tctttgacaa aaatagccag tacgaaccag gccgccaccg ccaccagtaa
    10501 aaaatagccc agcagcaacc gcatgccgat acgcattaca ggcccctcag gctatatccc
    10561 atgccgcgat gagtattaat cggtgaaaga tcggggttga tggcgcgcag cttggcacgc
    10621 aacgttttaa tgtgggtatc aacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg
    10681 ctatccatca gttgctggcg ggaccagacg cggcctggcg acttgagtaa cgtcttcagc
    10741 aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg
    10801 ggttcattca attcaaaatg accaatacgg atgacgggag acggcgacga gaacttcttc
    10861 acccgacgca gtaaggtgcg caccctggcg cacacttcgc ggggtgaaaa cggtttagcc
    10921 acatagtcgt cagcgccaat ttccagccca agcaggcgat cgacctcttc acttcgggcc
    10981 gtcaggaaca gtacaggtaa cgccggatgg agcgccagta actggcggca taattcaaag
    11041 ccgctgatat caggcaaacc aacgtcgaga atcatgacgt cgggaaccag ctggcgagct
    11101 ttatccagca ccggcaagcc tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac
    11161 atgtagacca gcgtgtcggc tatcccttgc tcatcttcca ctaaccagac cgtttcccgt
    11221 tgcataaatc gcctctgtta ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt
    11281 ttcggcgaac cttctacaac tttgtcggag taagccagat aagcgagcgc gttgcgttta
    11341 gcatcataaa agcgcacgac ctgtaacgac ttaaagacca gggacgtacg ttttttgaat
    11401 actacctcgc cctgagcttt gccgttttta atacgatccg acagttcaat cggcccgact
    11461 tgctgacaag aaatggccgc atcggaggta tcttccgcca ggcccaatcc ccctttaata
    11521 cccccggttt tcgcccggct cacataacag gtgacatttt tcacatcggg atcgtcaaag
    11581 gcttccacaa caattttgtg atccgggccg atcattttaa atacggtgtc gaccgaacca
    11641 atctcttctg catgagccaa tggccccagc attatcaggc ttaaagacag gatcaaatgc
    11701 ttgtatttca tattgttacc atttttatct attacacgag caattagttc gtcacggtaa
    11761 ataacatatt gattaagaac aaaatctcaa tacttttatt tccgtcaagc cctaaaacat
    11821 actctactaa gaaaaaaaca ctgaatgcta aaacagcaaa aaatgctatt atccaattac
    11881 ctgatgtcag gtgctcgttg ttgaaaggat gaggatattt tatggatcag gccggcatta
    11941 ttcgcgacct tttaatctgg ctggaaggtc atctggatca gcccctgtcg ctcgacaatg
    12001 tagcggcgaa agcaggttat tccaagtggc acttacagag aatgtttaaa gatgtcactg
    12061 gccatgctat tggcgcgtat attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac
    12121 gcctgactgc gcgtccgatt ctggacatcg cgctgcaata ccgcttcgac tctcaacaga
    12181 catttacccg cgcattcaag aagcagtttg cccagactcc tgcactttac cgccgttctc
    12241 ctgaatggag cgcctttggt attcgcccgc cgctacgtct gggtgaattc acaatgccag
    12301 agcacaaatt tgtcaccctg gaagatacgc cgctgattgg tgttacccag agctactcct
    12361 gttcgctgga gcaaatctct gatttccgcc atgaaatgcg ttatcagttc tggcacgatt
    12421 ttctcggcaa cgcgccgacc attccgccag tgctctacgg cctgaatgaa acgcgtccga
    12481 gtcaggataa agacgacgaa caagaggtat tctataccac cgcgttagcc caggatcagg
    12541 cagatggcta tgtactgacg gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt
    12601 ttacctatga aggtctggga accggcgtgc aggagtttat ccttacggta tacggaacgt
    12661 gcatgccaat gctcaatctg acgcgccgta aaggtcagga tattgagcga tactacccgg
    12721 cagaagatgc caaagcggga gatcgcccaa ttaatctgcg ctgtgaactg ctgattccga
    12781 tccgtcgtta acgctgcaac tcatctaatg caggggcgtc cagatgcgag atgtcccctg
    12841 cggtttcaac aacccagcca gacgccagcc acaggctttc ctgataatcc acgcgcgaaa
    12901 tagaacagtt acgcagacgt aagcggcgct ctgcccatgc tggtaatccg agaatcgtac
    12961 tcaccagaca tcccagtgca ataccatggc tcaccagcag cggtcggctt ccctgcggta
    13021 agtcccggca agattccagt gccgcattga cgcgatcgct gagctcctgc attgactcgc
    13081 cttcaggaat acgcccgtca acggtgccat tgaccagctg ccgacgccag ttctcttctt
    13141 cttcggtcag agaatcgata tgtctttttt ccagcacacc catgtttaat tcacgcaggc
    13201 gagaatcaaa gatgatgtca cagccgcagg cctgggcgat gatttccgcc gtacgccggg
    13261 tgcgtcctaa atcgctactg ataatatgcg taatgccaag ctctttggca cgggttgcca
    13321 cctgcatcgc ctgttgctca cctttggcgg tcagcgggct gtcagactgg ccctgaatac
    13381 gtcgctcggc gttccactgc gtttcaccgt ggcggactag gtatacctgt aacatgcttt
    13441 ttttccgtat actgctgtaa cgttaatttt ctgagtaatg ctgattatgc accaagttgt
    13501 ctgtgcgacc accaatcccg ctaaaattca ggccattctg caggcatttc acgagatctt
    13561 cggcgaagga tcctgccata ttgcatccgt tgccgtcgag agcggtgtac cggaacagcc
    13621 ctttggcagt gaggaaacgc gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt
    13681 acttccagag gctgattttt gggtggcgat tgaagctggc atcgatggtg acagcacttt
    13741 cagttgggtg gtgattgaaa acaccagcca gcgcggcgag gcgcgttcgg ctactttgcc
    13801 gttacctgcg gtgattctgc agaaagtgcg tgaaggtgag gcgcttggtc ccgtgatgtc
    13861 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    13921 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    13981 tcataatgcc gtgtattaag cgcctgatgc gacgctg
//
LOCUS       DAZFRN010000128        13869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFRN010000128 DAZFRN010000000
VERSION     DAZFRN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.130"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            94..615
                     /gene="fecI"
                     /locus_tag="ACHKUB_003728"
     CDS             94..615
                     /gene="fecI"
                     /locus_tag="ACHKUB_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO6656690.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGASSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            612..1565
                     /gene="fecR"
                     /locus_tag="ACHKUB_003729"
     CDS             612..1565
                     /gene="fecR"
                     /locus_tag="ACHKUB_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO6656691.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSSERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGDVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TSEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQLDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1652..3976
                     /gene="fecA"
                     /locus_tag="ACHKUB_003730"
     CDS             1652..3976
                     /gene="fecA"
                     /locus_tag="ACHKUB_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO6656692.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDANRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4021..4923
                     /gene="fecB"
                     /locus_tag="ACHKUB_003731"
     CDS             4021..4923
                     /gene="fecB"
                     /locus_tag="ACHKUB_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO6656693.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4920..5918
                     /gene="fecC"
                     /locus_tag="ACHKUB_003732"
     CDS             4920..5918
                     /gene="fecC"
                     /locus_tag="ACHKUB_003732"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO6656694.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5915..6871
                     /gene="fecD"
                     /locus_tag="ACHKUB_003733"
     CDS             5915..6871
                     /gene="fecD"
                     /locus_tag="ACHKUB_003733"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO6656695.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6872..7639
                     /gene="fecE"
                     /locus_tag="ACHKUB_003734"
     CDS             6872..7639
                     /gene="fecE"
                     /locus_tag="ACHKUB_003734"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO6656696.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTQINHLAGRRLTELSGGQRQRVFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8197..8454)
                     /locus_tag="ACHKUB_003735"
     CDS             complement(8197..8454)
                     /locus_tag="ACHKUB_003735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656697.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8535..8684)
                     /locus_tag="ACHKUB_003736"
                     /pseudo
     CDS             complement(8535..8684)
                     /locus_tag="ACHKUB_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            8869..9954
                     /gene="dndB"
                     /locus_tag="ACHKUB_003737"
     CDS             8869..9954
                     /gene="dndB"
                     /locus_tag="ACHKUB_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndB"
                     /protein_id="HFO6656698.1"
                     /translation="MASVDADYCYSFPAIRGIQAGRPFYIATCPMRIIPKIFSFDEND
                     VPPELRAQRTLNKSRIPEMVRYLLENPKDYVFSALTASIAVDVQFDEFPGSNNLGTLC
                     VPMDAQILINDGQHRRKAIEDALDARPELGQDNIPVLFFVDEGLKRSQQMFADLNKYA
                     IRPSPSLASLYDHRDACSNLARYLAMSVEPFVGMTELEKSGISQRSNKLFTLSSIKQA
                     TRALLGKDPKEDNFEECTHIATQYWQAIFSVMPDWQLAAKKEVSPVQLRQDYVHAHGI
                     GLQALGQLGYTLLTDYPEQWPEKIAALKTFNWRRNNPDLIKRAMQHGKLSKTSTAIQL
                     TCNALKTALSIPLTPEEQELEAQMVVS"
     gene            9951..11573
                     /gene="dndC"
                     /locus_tag="ACHKUB_003738"
     CDS             9951..11573
                     /gene="dndC"
                     /locus_tag="ACHKUB_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HFO6656699.1"
                     /translation="MSKLVQAYDLAEYEDFINQEQFAGRPLAEYVAEVQRIYCADKRP
                     WVIGYSGGKDSSAVITLVYLALLGLPPEMRSKDIFVVSSDTLVETPVVVDLIKKTMLQ
                     IEAGAKRNGLPITQHAVTPKTNETFWVNLLGKGYPAPTRSFRWCTERMKINPVSDFIK
                     DKVSQFDEVIVVLGSRSSESASRAQVIAKHKIDGSRLARHTTLANAFIYTPIDTWDVE
                     DVWKLLRGAFRYAPEDIDEWESPWGGNNRPLWTLYMDSSAQGECPLVIDDSTPSCGNS
                     RFGCWTCTVVTKDKAMESLIKNGEEWMSPLLKYRDLLAFTTDPVNKDTFRNYKRRTGK
                     VSYQYAKESDEPGAERKHVPGPYWLKYRQQWLKELLEIERDLNAQGHTITLITKPELH
                     AIRQEWLKDPNEPDWYDTLPGIYREVYQQDLDWVVDDQSRFDASDADLLVQIAQGFDV
                     VPEMVMKLIELEVSMEGLSRRQGIFDKLGTILKQDWGTLEEIEQQQVTLQKRVERDIH
                     QEELTKLETELQVLQRRIVDASESSLLIGEEK"
     gene            11575..13575
                     /gene="dndD"
                     /locus_tag="ACHKUB_003739"
     CDS             11575..13575
                     /gene="dndD"
                     /locus_tag="ACHKUB_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndD"
                     /protein_id="HFO6656700.1"
                     /translation="MLIKQLVLHNFRVFNGTHTIDLAPRKRPHDLNPRPIVLFGGLNG
                     AGKTSILSAIRIALYGRLAFGLATQQQEYIEQLSALIHNGAYYIEQPEEASVELTFTY
                     NKGGHETEFTVTRTWKKGKRDRLSLQQDGQPLSELDYDQCQGFLNELIPHGIADLFFF
                     DGEKIAELAEDESGNILRTAVRRLLGLDLISKLRNDLMIFVKRQQSSQLASSQQQQIA
                     ELEKQSKELACQTEALLESADFAKSRIEFLSKDITHYEGLLNAQGGAFAQTKAQEKQK
                     VETLLKDKEHLEKALRQECDGSLPYALAPTILSHLLQQIADETQIKQAKSFEKELSQF
                     LTQLRNDIAFRSAGTGKIAAEAITDNLKDYMATKPKGDLLFDISEREAGMLQQSIEID
                     SKRALQRFELYRTQLSEIEQQLEQAAANIARAPEDEQLMDLFEKLRDLDRQREAQLQK
                     YRSLLEDAKRKKQQQLDCVRQIQKAHDAARYQHSYSSAFKNAQETINLLDRYSDVLTQ
                     ARVKTLSTNFELAYRKLARKEDLQLSAHINPQTFDVELIDENGSVINRKLLSAGEKQI
                     YAIAILEALAKTSGRDLPVIIDTPLGRLDSQHRDKLINHYFPEASHQVVLLSTDTEVD
                     ERYFVDQLRDDISHAYEIVFNAHTKSSTLKPGYFWELTKEAI"
     gene            13575..>13869
                     /gene="dndE"
                     /locus_tag="ACHKUB_003740"
     CDS             13575..>13869
                     /gene="dndE"
                     /locus_tag="ACHKUB_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndE"
                     /protein_id="HFO6656701.1"
                     /translation="MLPNRMALSRQTEDQLKKLKGYTGITPNIAARLAFFRSVESEFR
                     YSPERDSKKLDGTLVLDKITWLGETLQATELVLKMLYPQLEQKALIKAWAAHVE"
BASE COUNT         3326 a         3567 c         3736 g         3240 t
ORIGIN      
        1 atgtctattg gaaacaattt tatttccaat tgtaatgata accattctca tattaatatg
       61 actacgtgat aattaacttt tgatgcactc cgcatgtctg accgcgccac taccacagct
      121 tccttaacgt tcgagtcgct ttatggcaca catcacggct ggttgaaaag ctggctgacg
      181 cgcaaactcc agtctgcttt tgatgcagat gacattgccc aggacacttt tttgcgggta
      241 atggtcagcg aaacgctctc gacgatccgc gatcctcgct ccttcctctg cactatcgcc
      301 aaacgcgtga tggtggacct gtttcgccga aacgcgctgg aaaaagcgta tctggagatg
      361 ctggcgctta tgccggaggg gggagcgtct tcacctgagg aacgcgaaag ccaactcgag
      421 accctacaac tcctcgacag catgctggac gggctaaacg gcaaaacacg tgaagcgttt
      481 ctgctttcgc aactggatgg tctgacatac agcgagattg cgcacaaact cggtgtttcc
      541 atcagctccg tgaaaaaata cgtggcgaaa gccgtcgagc actgcctgct gttccgtctg
      601 gagtatgggt tatgaatcct ttgttaaccg attcccgccg tcaggcgctg cgttcagctt
      661 cccactggta tgccgtgcta agcagcgagc gcgtcagccc acaacaggaa gcgcgctggc
      721 aacagtggta tgaacaggat caggataacc agtgggcctg gcagcaggtt gaaaacctgc
      781 gcaaccagct tggcgatgtg cctggcgacg ttgccagccg ggcgttgcac gatacccgcc
      841 tcacccgccg tcacgtgatg aaaggattac tgctgttgct cggcgctggc ggaggctggc
      901 agctctggca gtcggaaacc agcgaaggtc tgcgggcaga ttaccgcacc gccaaaggca
      961 cagtcagccg tcagcaactg gaagatggct ccctgctcac gctgaatacc caaagcgcgg
     1021 cggatgtgcg ttttgatgcg catcagcgca ccgtccggct ctggtacggt gaaatcgcca
     1081 ttactaccgc gaaagatgcc ctgcaacgcc ccttccgtgt cctgacccgt cagggccagc
     1141 tcactgcttt agggacagaa tttaccgtcc gccagctgga taatttcacg cagcttgacg
     1201 ttcagcagca cgctgtggaa gtgcttctcg ccagtgcccc cgcgcaaaaa cgcatcgtga
     1261 acgctggtga aagcctgcag ttcagcgcct ctgagtttgg cgcagtgaaa ccgctggatg
     1321 acgagagtac aagctggacg aaggacatcc tgagcttcag cgataaaccg ctgggtgagg
     1381 tgatagccac gctaacccgt taccgcaacg gcgtgctgcg ctgcgatccc gccgttgccg
     1441 ggctgcgcct gagcgggacg ttcccgctga aaaataccga tgcgatcctg aacgttatcg
     1501 cgcaaacgct tcccgttaaa attcagtcta ttacgcggta ctggataaac atttcaccac
     1561 tgtaaggaaa ataattctta tttcgattgt cctttttacc cttctcgttc gactcatagc
     1621 tgaacacaac aaaaatgatg atggggaagg tatgacgccg ttacgcgttt ttcgtaaaac
     1681 aacacctttg gttaacgcca ttcgcctgag cctgctgccg ctggccggtc tctcgttttc
     1741 cgcttttgct gcacaggtta atatcgcacc gggatcgctc gataaagcgc tcaatcagta
     1801 tgccgcacac agcggattta ccctctcggt tgacgccagc ctgacgcgcg gcaagcagag
     1861 caacggcctg cacggcgatt acgacgtcga gagcggcctg caacaactgc tggacggcag
     1921 cggactgcag gtaaaaccgc tgggaaataa cagctggacg ctggagcccg cgcccgcacc
     1981 aaaagaagat gccctgaccg tggtcggcga ctggctgggt gatgcgcgtg aaaacgacgt
     2041 atttgaacat gctggcgcgc gtgacgtgat ccgccgtgag gatttcgcca aaaccggcgc
     2101 aaccaccatg cgtgaggtac ttaaccgcat ccctggcgtc agcgcgccgg aaaacaacgg
     2161 caccggcagc cacgacctgg cgatgaactt tggcatccgg ggcctgaacc cgcgcctcgc
     2221 cagccgctcg accgtcctga tggacggcat ccccgtcccc ttcgcccctt acggtcagcc
     2281 gcagctttca ctggctcccg tttcgctcgg caacatggat gccattgacg tggtacgcgg
     2341 tggtggtgcg gtgcgttacg gaccgcagag cgtgggcggc gtggtgaact ttgttacccg
     2401 tgccattccg caggactttg gtatcgaggc gggcgtggaa ggtcagctca gcccaacctc
     2461 ttcacaaaac aacccgaaag agacgcacaa cctgatggtg ggcggcacag cggacaacgg
     2521 ttttggcacc gcgctgctct actccggcac gcgcggcagt gactggcgcg agcacagcgc
     2581 cacccgcatc gacgacctga tgctgaaaag caaatatgcg ccggatgagg tgcacacctt
     2641 caacagcctg ctgcaatatt acgacggtga agccgacatg cccggtggcc tgtctcgcgc
     2701 ggattacgac gccaatcgct ggcaatccac ccgcccgtat gaccgcttct ggggtcgtcg
     2761 caagctggcg agcctgggct accagttcca gccagacagc cagcataaat tcaacattca
     2821 ggggttctac acccaaaccc tgcgcagcgg ctacctggag caaggcaaac gcatcaccct
     2881 ctcgccgcgt aactactggg tgcgcggtat tgagccacgc tacagccaga tctttatgat
     2941 cggcccttcc gcgcacgaag tgggcgtggg ctatcgctat gtgaatgaat caacgcatga
     3001 aatgcgttac tacaccgcca ccagcagcgg gcagttgccg tccggctcaa gcccttacga
     3061 ccgcgatacg cgttccggca ccgaggcgca cgcctggtat ctggatgaca aaatcgacat
     3121 cggcaactgg accatcacgc cgggtatgcg tttcgaacat atcgagtcat accagaacaa
     3181 cgccatcaca ggcacgcacg aagaagtgag ctataacgca ccgcttccgg cgttgaacgt
     3241 gctctatcac ctgactgaca gctggaatct ttatgcaaac actgaaggct cgttcggcac
     3301 cgtacagtac agccagattg gcaaggctgt gcaaagcggc aatgtggaac cggaaaaagc
     3361 gcgaacctgg gaactcggta cccgctacga cgacggcgcg ctgacggcgg aaatggggct
     3421 gttcctgatt aactttaaca atcagtacga ctccaaccag accaacgaca ccgtcactgc
     3481 acgtggcaaa acgcgccata ccgggctgga aacgcaggca cgttacgatc tgggtacgct
     3541 aacgccaacg cttgataacg tttccatcta cgccagctat gcgtatgtga acgcggaaat
     3601 ccgcgagaaa ggcgacacct acggcaatct ggtaccattc tccccgaaac ataaaggcac
     3661 gctgggcgtg gactacaagc caggaaactg gacgttcaat ctgaacagcg atttccagtc
     3721 cagccagttt gcggataacg ccaatacggt gaaagagagc gccgacggca gtaccggccg
     3781 cattcccggc ttcatgctct ggggcgcacg cgtggcgtat gactttggcc cgcagatggc
     3841 agatctgaac ctggcattcg gtgtgaaaaa catcttcgac caggactact tcatccgctc
     3901 ttatgacgac aacaacaaag gcatctatgc aggccagccg cgcacgctgt atatgcaggg
     3961 gtcgttgaag ttctgatgtg ttaacgcccg gcttgccggg cttttagctg gaatgtgatt
     4021 atgttggcat ttatccgttt tgtttttgca ggcctgctgc tggtgatcag ccacgccttt
     4081 gccgccacgg ttcaggacga acacggcacg tttacactcg aaaaaacgcc acaacggatt
     4141 gtggtgctgg aactctcgtt cgccgatgcg ctggccgccg tggacgtcag cccgatcggt
     4201 attgccgacg ataacgatgc aaaacgcatc ctgcccgaag tgcgtgcgca cctgaaaccg
     4261 tggcagtccg tcggaacgcg cgcgcagccg agcctggaag ccattgccgc tctgaaacca
     4321 gacctgatca ttgccgacag cagtcgccat gcgggggttt acatcgcctt gcagcaaatc
     4381 gcgccggtac tgctgcttaa gtcccgcaac gaaacctacg ctgaaaattt gcaatctgcg
     4441 gctatcatcg gcgaaatggt gggtaaaaag cgagagatgc aggcacgcct ggaacaacat
     4501 aaagagagga tggcgcagtg ggccagccag cttcccaaag ggacacgcgt ggcctttggc
     4561 acatcacgcg aacagcaatt caacctgcat actcaggaga cctggaccgg cagcgtgctg
     4621 gcctctctgg ggctgaacgt tcccgctgcg atggcgggtg cgtccatgcc gtccatcggc
     4681 ctggagcaac tgctggcggt caatcctgcc tggctgctgg ttgcccacta tcgcgaagag
     4741 agcattgtta aacgctggca acaagatccg ctctggcaga tgttaaccgc cgcgcagaag
     4801 cagcaggttg cttcggtcga cagtaacacc tgggcgcgga tgcgcggtat ttttgctgca
     4861 gagcgtattg ccgctgacac ggtaaaaatc ttccaccatc agccgcttac cgttgtgaaa
     4921 tgaccgcgat aaaacacccg gtgctgctgt gggggcttcc cgttgcagca cttattatta
     4981 ttttctggct gagtctgttt tgctactcgg ccattcctgt ttccggagca gatgcaaccc
     5041 gcgccctgct gcctggacac acgccaacgc taccagaagc gctggtgcaa aaccttcgtt
     5101 tgccacgaag cctggtcgcc gttctgatcg gcgcaagcct ggcgctcgcg ggcacgctgc
     5161 tgcaaaccct gacccacaac ccaatggcct ctccttcact gctcggcatt aacagcggcg
     5221 cggcgctggc tatggcgctt accagcgcgc tgagtccgac gccgattgca ggctattctc
     5281 tgtcgttcat cgcggcatgc gggggcggcg tgagctggct gctggtcatg accgcaggag
     5341 gcggatttcg tcatacccat gacagaaaca aactgatcct cgcgggtatc gcgctgtcgg
     5401 ccttttgtat gggccttacc cgcatcaccc tgctgctggc cgaagatcat gcttacggca
     5461 tcttttactg gctggcaggc ggagtgtccc acgcccgctg gcaggatgtc tggcagctct
     5521 tgccggtggt ggtcactgca gtccctgtcg tgttgctgct ggcgaatcaa ctgaacctgc
     5581 tcaacctcag cgacagcacc gcccatacgc tgggagtgaa cctgacgagg ctacgtttgg
     5641 tcatcaatat gttagtgctg cttctggttg gtgcgtgcgt cagcgtggca ggtccggtgg
     5701 cgtttatcgg tctgctggtg ccacatctgg cgcgcttctg ggcaggcttc gatcagcgca
     5761 acgtactgcc agtgagcatg ctgctggggg ccacgctgat gctgctggca gatgtactcg
     5821 cacgcgcgct ggccttcccc ggagatctgc ccgcaggcgc agtgctggcg ctgattggca
     5881 gcccttgctt tgtctggctt gtgaggaggc gaggatgaaa attgcgctgg ttattttcat
     5941 cacccttgcc ctggcgggct gtgcgctgtt atcactccat atgggagtga tccccgtgcc
     6001 gtggcgcgcg ctgctgaccg actggcaggc cggacgcgag cattattatg tattgatgga
     6061 gtaccgactg ccgcgcttgc tgctggcact gtttgtcggt gcagccctcg ccgtggcggg
     6121 cgtgctgata caggggattg tgcgcaaccc tctggcatca ccggatattc tcggcgttaa
     6181 ccatgccgcc agcctggcct ctgtgggggc tctacttctt atgccgtcac tgcccgtgat
     6241 ggtgctgccg ctgctggcct ttgcgggcgg catggcgggg ttgatattgc tgaagatgct
     6301 ggcaaagacc caccagccga tgaagctggc gctcaccggc gtggcgcttt ctgcatgctg
     6361 ggccagcctg acggattatc tgatgctctc gcgcccgcag gatgtgaaca acgccctgct
     6421 gtggctgacc ggcagcttat ggggccgtga ctggagcttt gtgaagattg ccatcccgct
     6481 gatgatttta tttctgccgc tgagcctgag tttttgccgc gatctcgacc tccttgcact
     6541 cggcgatgcg cgcgccacca cgctcggtgt gtcggtgccc catacccgat tctgggcttt
     6601 gttactagct gtcgccatga catctaccgg cgtggccgcc tgcggcccga ttagctttat
     6661 tggtctcgtg gtgccgcata tgatgcgtag catcaccggt ggacgtcacc gcagactgct
     6721 gcctgtttca gccctgacag gtgcgttgct gttggtggtt gccgatctgc tggcgagaat
     6781 tattcatccc ccactggagc tcccggttgg cgtgctgacc gccattatcg gtgcgccgtg
     6841 gtttgtctgg ttgcttgtga gaatgcgata aatgacttta cgaactgaaa atctgacggt
     6901 cagttacggg acagacaagg tacttaacga cgtttcactc tcactgccaa cggggaagat
     6961 caccgccctg atcggtccta acggttgcgg gaaatcgacg ctgttaaact gtttttcgcg
     7021 gcttttaatg ccgcagtctg gcaccgtatt tctcggcgat aatcccataa atatgctctc
     7081 atcgcgccag ttggcccgca ggctttcgct gctgcctcag caccatttaa cgccagaggg
     7141 gatcacagtc caagagctgg tttcgtatgg tcgtaatccc tggctgtcac tctgggggcg
     7201 tctctccgct gaagacaatg cacgagttaa tgtcgccatg aaccagaccc agatcaatca
     7261 tcttgccggt cgtcggttaa ccgagctttc cggcggtcag cgccagcgcg tatttctggc
     7321 gatggtcctg gcccagaata cgcccgttgt attacttgat gagccaacca cctatcttga
     7381 tatcaatcac caggtggacc tgatgcggtt gatgggcgaa ctccggactc aggggaaaac
     7441 ggtggtcgct gtgctgcacg accttaatca ggctagccgg tactgcgatc aactggtggt
     7501 aatggcaaac ggacatgtta tggcgcaagg cacaccagaa gaggtgatga ccccaggatt
     7561 gctgagaaca gtattcagcg tggaagcgga aatacacccc gagccggtat ctggcaggcc
     7621 gatgtgccta atgaggtaga ttgcacaggc cgtaagaacc aaaccacgac tgaatgaaac
     7681 tggactggcg ccagcaagcc tgttcagact ggggctgaac ttttccggac tctgaaagat
     7741 taccaatact catcgtccat ccgcttgctt taggctgaca ggttcataat caacgcaaac
     7801 cagagctgta caggcttggg cgcggctttc aaaccagtcg tgatcacggc aatcaatttt
     7861 gaactctgct taacggacat ttctgtataa cccttacggc aacgaaaaac gcgaagttaa
     7921 aattttagaa acccaaaaac gtgacatgac taagtttaga tttcaggggg ggagatcaaa
     7981 aaatttcgct ctgtgccaga gcggacattc acggagctgg ttcattacca atgaggttgg
     8041 gcttttgagg ataaatcaat gatcagacgc caacgtaaat caaaagcaac cctggaacgg
     8101 aattgctaat ccagtttctg accatcgatt tttctaaaaa gtgtgcgttt gctactactt
     8161 aggtaggtgc agctttctta atcaccggca gccacgttat acaggccagt tgatggatcg
     8221 attgttatca atgatatctt catgagtcgg tgtctcaccc agcttaccga agctggcata
     8281 aagtgaaggc agacgggccc gtccttctcc ctttttcgcc agagggaaag cgcgaagcat
     8341 ggtggcagcc tcccagtcac aacaatagga tggtgtgcac ggctgctgac gccatgattc
     8401 agcgatagag ccggaaaata cggggtcaaa gccggtatca ttaaccagag tcatcgtgac
     8461 ctgtactgcg gctggatgat ctcctgctac tgcaactgct aggcgaccgc ggctcccttc
     8521 aggtagtgaa gtggtcaact taaactggcc accgatttag agtttttcca gtagcggtgt
     8581 tccgattcgt ttgggggcaa cccgccgtta ttgtcatgcg gtcttagcga gctgtaatac
     8641 ccgatgatat agtctgtgat cgcatgagca gcttcgctaa agcttatgcg ttagcgctct
     8701 acagaagtac agagcatgtc tgcacggttg ctctgggtta tagagcaaaa ttgcgggtat
     8761 attgaaaggt ggtacgcaat ttattacgtg aaaaagctgt aatttctagc cagtgttacg
     8821 taaaatataa cgtaacaaaa ttcaccttat gcttttatgg attacagcat ggctagtgtt
     8881 gatgcagatt actgctactc gtttccggct atacgtggca ttcaggctgg tcgccctttc
     8941 tatatcgcca catgcccgat gcgtatcatc ccgaaaatat tcagttttga tgaaaatgat
     9001 gtgccaccgg aattacgcgc acagcgcacg ctaaataaat cgcgcattcc cgagatggta
     9061 cgttatttgt tggaaaaccc taaagattat gtgttttcag ctctgacggc ttccattgcc
     9121 gtagacgttc aatttgatga gtttccaggt tctaacaatc tggggacgtt gtgcgtacca
     9181 atggatgcgc aaattctaat caatgatgga caacatcgcc gtaaagcgat tgaagacgct
     9241 ttagacgctc gtccggagct gggccaggat aatatcccgg tgttgttctt tgtcgatgaa
     9301 ggcttaaagc gtagccagca gatgttcgcc gatctgaata aatacgccat tcgccccagc
     9361 ccatcgctgg cgtcgctata cgatcatcgc gatgcctgtt cgaatctggc gcgttatctg
     9421 gcaatgtcag tagagccgtt tgttgggatg acggaactgg aaaaatctgg cattagccag
     9481 aggtcgaata agctatttac gttgagcagc atcaagcagg ctacccgcgc attgttgggc
     9541 aaagatccca aagaggataa ctttgaggaa tgcacccaca ttgctaccca atattggcag
     9601 gctattttta gtgtaatgcc tgattggcaa ttagcggcta aaaaggaagt ttcaccggtt
     9661 caattgcgac aagattatgt tcacgctcat ggcattggat tgcaggcact tgggcaacta
     9721 ggatataccc tgctgacgga ttatcctgag caatggccgg agaaaatcgc tgcactcaaa
     9781 acatttaact ggcgtagaaa caatccggac ctcatcaagc gagcgatgca gcatgggaag
     9841 ttgagtaaaa ccagtaccgc gatccagtta acctgcaatg cgctaaaaac cgcactctca
     9901 attcccctca ctcctgaaga acaggagctt gaagctcaaa tggttgtctc atgagtaaat
     9961 tagttcaggc ctatgatttg gccgaatacg aagattttat taatcaggaa cagttcgccg
    10021 gacgtcctct ggccgaatat gttgctgagg ttcaacgaat ttattgtgcg gataaacgtc
    10081 cgtgggtgat tggctacagc ggtgggaaag attcttccgc tgtgatcacg ctggtgtatc
    10141 tggccttgct cggcctgccc ccggaaatgc gcagcaaaga tatttttgtg gtgtcgtccg
    10201 atacgctggt cgaaacaccg gtagtcgtgg atctgattaa gaaaacgatg ttgcagattg
    10261 aggcgggagc gaagcgtaac ggcttgccga tcactcaaca cgcggtaacg cctaaaacga
    10321 atgaaacctt ctgggtcaat ttgctgggga aaggttatcc ggctccaact cgtagtttcc
    10381 gttggtgcac tgagcgcatg aaaatcaacc cggtgagtga ctttattaaa gataaggtca
    10441 gccagtttga tgaagtcatc gtcgtacttg gttcgcgcag tagtgaaagt gcttctcgtg
    10501 cgcaggtgat cgcaaagcac aagattgatg gatcacgcct tgcccggcat accacgctgg
    10561 caaatgcatt catctatacc cccattgata cctgggatgt agaagatgtc tggaagctgc
    10621 tgcggggcgc gttccgctat gcccctgaag atatcgacga gtgggaaagc ccgtggggtg
    10681 gaaacaaccg tccactgtgg acgttgtata tggattcttc cgcacagggt gaatgtcctc
    10741 tggtaattga tgatagtacc ccttcctgcg gcaactcgcg ttttggctgt tggacctgta
    10801 ccgttgtcac taaagataag gcgatggaaa gcctgatcaa aaacggtgaa gaatggatgt
    10861 ctccgttgct gaaatatcgt gatctgctgg catttaccac cgatccggtg aataaagaca
    10921 cgtttcgaaa ctataagcgc cgtactggta aggtgagcta tcagtacgct aaagaaagtg
    10981 atgagccggg ggctgagcgt aagcatgttc caggtcctta ctggctaaaa taccgtcagc
    11041 aatggctcaa agaattgctg gagatagagc gcgatttgaa tgctcaaggc cataccatta
    11101 cgttaattac caaaccagag ctgcatgcga ttcgtcagga gtggttgaaa gatcctaatg
    11161 aaccagactg gtatgacacg ctgccgggta tttaccgtga agtgtatcaa caggatttag
    11221 actgggtggt ggacgatcag tcgcgctttg atgccagcga tgcagatctg ttggtgcaga
    11281 tagcgcaggg atttgacgtt gtgcctgaaa tggtgatgaa actcatcgag ctggaagttt
    11341 caatggaagg cttaagccgt cgtcagggca tttttgacaa acttggcacg attttaaagc
    11401 aggactgggg cactctcgaa gaaattgaac aacaacaggt cactctgcaa aaacgagttg
    11461 aacgagatat tcatcaggaa gaacttacaa agctggaaac tgaacttcag gttttacagc
    11521 gcagaattgt tgatgccagt gaatcctcgt tactgattgg ggaggagaaa taacgtgctc
    11581 attaaacagc tggtattaca taattttcgg gtatttaacg gtacacatac cattgatctg
    11641 gcacccagaa agcgtccgca cgatttgaat ccacgaccta ttgtgctgtt tggtggttta
    11701 aacggcgcag ggaaaacgtc tatcctgtcg gcgatccgca tagcgttgta tggacgactg
    11761 gcgtttgggc tagcgactca acagcaagag tatatcgaac aactcagtgc attgattcat
    11821 aacggcgcgt actatattga gcagccggaa gaagcatctg tagaactgac gtttacctat
    11881 aataaaggcg gtcatgagac cgaatttacc gttacgcgaa cctggaaaaa ggggaagagg
    11941 gaccgccttt ctctgcaaca ggatgggcag ccactcagtg agctggatta cgatcagtgc
    12001 cagggatttc taaatgagtt aattccgcat ggcattgctg atttgttctt tttcgatggc
    12061 gagaaaattg cagaacttgc tgaggacgaa tccggtaata ttttacgtac tgccgtacga
    12121 cgcttattgg ggctggatct catctctaaa ctgcgtaacg atttaatgat ttttgttaag
    12181 cgtcagcaat ccagccagct agcaagttct cagcaacaac agattgcaga gcttgaaaaa
    12241 cagagtaaag aattagcctg ccagacagaa gcactgctgg aaagcgccga ttttgccaaa
    12301 tcgcggattg agtttctatc taaagatata acccattatg aaggcttgct aaatgctcag
    12361 gggggcgctt ttgcccagac taaagcgcag gaaaaacaga aagtcgaaac gctgctaaaa
    12421 gataaagaac atctggaaaa agcactgaga caagaatgcg atggctcgct accttatgcg
    12481 cttgcgccca ctattttgtc tcatttgctg caacaaattg ctgatgaaac gcaaattaaa
    12541 caggcaaaaa gctttgaaaa agagctgagc cagtttttaa cgcaactcag aaatgatatt
    12601 gcatttcgtt ctgcggggac tggcaaaata gcagctgaag cgattaccga taatctaaaa
    12661 gattatatgg caacgaaacc aaaaggtgat ttgctctttg atatctctga gcgtgaagca
    12721 ggtatgcttc aacagtctat tgagatagat agtaaaagag cattgcagcg tttcgagctt
    12781 tatcgcacac agttatctga gattgaacaa cagttagaac aagcggctgc taatattgcc
    12841 cgcgctcctg aagatgagca gctgatggat ctctttgaaa aattacgtga cctggatagg
    12901 cagcgcgaag cccaattgca gaaatatcgg tcattgctag aagatgctaa acgtaaaaaa
    12961 cagcagcaac tggattgtgt tcgtcagatt caaaaagcgc atgacgccgc tcgttatcag
    13021 cacagttata gcagtgcgtt taagaatgct caggaaacga taaatttact cgatcgctac
    13081 agcgatgtat taacccaggc tcgggtaaaa acgctgtcga caaacttcga gcttgcttac
    13141 cgtaagttgg ctcgcaaaga ggatttacaa cttagtgcgc atatcaaccc tcaaacgttt
    13201 gatgttgaat taattgatga gaatggctca gtaattaacc gtaagttact ttctgctggt
    13261 gaaaaacaga tttatgcaat tgctattctt gaggcgctgg cgaaaacctc tggccgtgat
    13321 ttaccagtga tcattgatac accattgggt cgtctggact ctcaacatcg agataaattg
    13381 attaatcact acttccctga agccagtcat caggttgtgt tgctttctac cgatactgaa
    13441 gtagatgaac gttattttgt tgatcagttg cgtgatgata tctctcatgc ttatgagatt
    13501 gtgtttaatg cacatactaa gtcatcaacg ctgaagcctg gttatttctg ggaattaact
    13561 aaggaggcta tctgatgctc ccgaatcgaa tggcgcttag ccgtcagact gaagatcagc
    13621 ttaaaaagct taaagggtat actgggatta cacccaatat tgctgcccgg ctagcatttt
    13681 tccgctcggt ggagagtgag tttcgctatt cacctgaacg agatagtaag aagctggatg
    13741 gtacgctggt gctggataaa attacgtggc tgggggagac actacaagct acagaattgg
    13801 tgctgaagat gctttatcca caattagagc aaaaggcgtt gattaaggca tgggctgctc
    13861 atgttgagg
//
LOCUS       DAZFRN010000129        13567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFRN010000129 DAZFRN010000000
VERSION     DAZFRN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.131"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            55..2283
                     /gene="crfC"
                     /locus_tag="ACHKUB_003741"
     CDS             55..2283
                     /gene="crfC"
                     /locus_tag="ACHKUB_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO6656702.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSAVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2280..3158
                     /gene="rdcB"
                     /locus_tag="ACHKUB_003742"
     CDS             2280..3158
                     /gene="rdcB"
                     /locus_tag="ACHKUB_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO6656703.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            3422..4924
                     /gene="proP"
                     /locus_tag="ACHKUB_003743"
     CDS             3422..4924
                     /gene="proP"
                     /locus_tag="ACHKUB_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO6656704.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5036..5125
                     /gene="pmrR"
                     /locus_tag="ACHKUB_003744"
     CDS             5036..5125
                     /gene="pmrR"
                     /locus_tag="ACHKUB_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO6656705.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5101..6201)
                     /gene="pmrB"
                     /locus_tag="ACHKUB_003745"
     CDS             complement(5101..6201)
                     /gene="pmrB"
                     /locus_tag="ACHKUB_003745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO6656706.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6202..6870)
                     /gene="basR"
                     /locus_tag="ACHKUB_003746"
     CDS             complement(6202..6870)
                     /gene="basR"
                     /locus_tag="ACHKUB_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO6656707.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6867..8510)
                     /gene="eptA"
                     /locus_tag="ACHKUB_003747"
     CDS             complement(6867..8510)
                     /gene="eptA"
                     /locus_tag="ACHKUB_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO6656708.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNITALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8614..9951)
                     /gene="adiC"
                     /locus_tag="ACHKUB_003748"
     CDS             complement(8614..9951)
                     /gene="adiC"
                     /locus_tag="ACHKUB_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO6656709.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            complement(10088..10849)
                     /gene="adiY"
                     /locus_tag="ACHKUB_003749"
     CDS             complement(10088..10849)
                     /gene="adiY"
                     /locus_tag="ACHKUB_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO6656710.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11174..13441)
                     /gene="adiA"
                     /locus_tag="ACHKUB_003750"
     CDS             complement(11174..13441)
                     /gene="adiA"
                     /locus_tag="ACHKUB_003750"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO6656711.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3278 a         3326 c         3550 g         3413 t
ORIGIN      
        1 aaaacaatct ggctaaataa aagtaacaaa atttgcttta aggaagaatt ttctatgtac
       61 acacagaccc tgtatgagtt aagtcaggag gctgaacgcc tgttacagct ttctcgccaa
      121 cagttgcagt tactggaaaa aatgcctctc tctgtacccg gagacgatgc gccacaactg
      181 gctttaccct ggagtcagcc taatatcgcc gaacgtcacg cgatgctgaa taatgagttg
      241 cgtaaaattt cccgactgga aatggtgctg gcgattgtcg gtaccatgaa agcagggaaa
      301 tcaaccacca ttaatgccat tgttggtacg gaggttttgc ctaatcgtaa tcgcccaatg
      361 actgcgctgc cgacgcttat tcgccatacg cccgggcaaa aagaaccggt actgcatttt
      421 tcacatgtcg cgccaatcga ccgtttaatt caaaaattac aacagcgcct gcgtgattgc
      481 gatattaagc atctgaccga tgtgctggaa atagataaag atatgcgtgc gcttatgcag
      541 cggatcgaaa atggcgtcgc tttcgaaaaa tattatctgg gtgcccagcc tatttttcat
      601 tgtctgaaaa gtttgaatga tttagtgcga ctggcgaagg cgctggacgt cgattttcct
      661 ttttctgctt acgccgccat tgagcatatt cccgtgattg aagtggagtt tgtccatctg
      721 gcggggctgg aaagttatcc cggtcagttg acgttactgg atacccccgg gccaaatgaa
      781 gccgggcaac cgcatctgca aaaaatgctt aaccagcagc tggcacgcgc ctcggcggta
      841 ctggcggtgc tggattatac gcaactgaaa tcgatctccg atgaagaggt ccgtgaggcg
      901 attttggcgg tggggcaatc ggtgccgctg tatgtgctgg tcaataagtt cgatcaacag
      961 gatcgtaaca gtgacgacgc cgaccaggtg cgggcactga tttccgggac gctgatgaaa
     1021 ggctgtatta cgccacagca gatatttccg gtgtcgtcga tgtggggcta cctggcgaat
     1081 cgggcgcgcc atgagttagc caacaacggt aagttaccac cgccagagca acaacgctgg
     1141 gtggaagatt ttgcccatgc cgcgctcggc aggcgctggc gtcatgccga tctggcggac
     1201 ctcgaacata ttcgtcatgc tgccgatcag ttgtgggaag attcgctgtt cgcccagcca
     1261 attcaggcgt tgcttcatgc cgcttacgct aacgcctcgt tgtatgctct gcgatctgcc
     1321 gcgcataaac tgttgaatta cgcgcagcag gcgcgggaat acctggattt tcgtgcgcac
     1381 gggttaaacg tcgcttgtga acaattgcgg caaaatatcc atcaggtcga agaaagtttg
     1441 cagctattgc aactcaatca ggcgcaggtg agcggcgaga ttaaacatga aatcgagctg
     1501 gccctgacct ccgccaacca ctttctgcgt caacagcaag atgcggtgaa tgcacagtta
     1561 gccgcgttgt ttcaggatga ttcggagccg ttaagcgaga ttcgtacccg ctgtgagaca
     1621 ctgttacaga cggcgcagaa caccatcagt cgcgacttta cgctgcgttt tgccgagctg
     1681 gaatccaccc tttgccgggt gttaaccgat gttattcgcc ccattgagca acaagtcaaa
     1741 atggaattga gcgagtcagg gtttcgtcct gggtttcatt ttcctgtttt tcacggcgca
     1801 gttccccact tcaacactcg ccagctgttc agtgcagtca tttcgcgcca ggaagcaacg
     1861 gacgagcaga gcacgcgttt aggcgttgtg cgtgagactt tttcgcgctg gttgaatcag
     1921 cccgactggg gacggggaaa tgagaaatcc ccgacagaga cggttgatta cagtgtgttg
     1981 caacgagctt taagcgcaga agtcgatctt tattgccaac aaatggctaa agttctggca
     2041 gagcaggtcg atgaatctgt tacggcaggc atgaatactt ttttcgctga gttcgcttca
     2101 tgtttgacgg aattacagac gcgtttacgc gaaagtctgg ctctgcgtca acaaaatgaa
     2161 tcggtggtca ggctgatgca gcagcaattg cagcggactg tgatgactca cggctggatt
     2221 tacaccgacg cccagctgtt acgcgatgat attcaaacac ttttcacggc agaacgatat
     2281 tgaccaagac gttacttgac ggccccggtc gcgtgctgga gtcggtttat ccccgctttt
     2341 tagtggatct ggcgcagggc gatgatgccc gccttccaca agcccatcag cagcagtttc
     2401 gtgaacgact gatgcaggaa cttcttgcgc gcgtgcagct tcagacatgg acgaacggcg
     2461 gcatgttaaa tgcgccgctt agcctgcgtc tgacattggt ggaaaaactg gcgtcgatgc
     2521 tggatcccgg tcatctggca ctgacgcaga tcgcgcagca tctggcgctg ctgcaaaaaa
     2581 tggatcaccg ccagcactct gctttcccgg agctccccca gcaaattgcc gccttgtatg
     2641 agtggttttc agcccgttgc cgctggaagg aaaaggcgtt aacgcaacga ggcctactgg
     2701 tgcaggcagg tgatcagagc gagcaaattt ttacccgctg gcgtgctggg gcgtataacg
     2761 cctggtcgtt gcctgggcgc tgttttatcg ttctcgaaga gttgcgttgg ggggcatttg
     2821 gcgatgcctg tcgtctggga agcccgcaag cggtggcgtt gttgctgggt gatttgcgcg
     2881 tgaaagcgac acaacatctg gcagagagta tcaatgcggc cccgaccacg cgtcactatt
     2941 accatcagtg gtttgcctct tcgaccgttc cgacgggcgg ggatcatgct gattttttaa
     3001 gttggctggg aaagtggacc acggcagata aacaacccgt ttgctggtca gtgacccaac
     3061 gctggcaaac tgtcgcgctg gggatgccac gactctgttc agcgcagcgt ctggcggggg
     3121 caatgctcga ggaaatcttc tctgtaaatt tggcgtaaat aatcagttac atcaatgagt
     3181 cctaaacgaa atccatgtgt gaagttgatc acaaatttaa acactggtag ggtaaaaagg
     3241 tcattaactg cccaattcag gcgtcaactg gtttgattgt acattcctta accggagggt
     3301 gtaagcaaac ccgctacgct tgttacagag attgcatcct gcaattcccg ctcccctttt
     3361 gcggccgtcg cgctgatttt tctggcgttt gcggaaatgg gccaactctg cgaggaaagc
     3421 tatgctgaaa aggaaaaaag taaaaccgat tacccttcgt gatgtcacca ttattgatga
     3481 cggtaaactg cgtaaagcca ttaccgcagc atcactgggt aatgcaatgg aatggtttga
     3541 ttttggtgtt tatggttttg ttgcttacgc attaggtaaa gtttttttcc cgggggctga
     3601 ccccagcgtg cagatggttg ctgcacttgc cacgttctcc gttccctttc tgattcgacc
     3661 gcttggcgga ctcttctttg gtatgttggg cgataaatat ggtcgccaga agatcctcgc
     3721 tatcactatt gtgattatgt cgatcagtac gttctgtatt ggcttaatac cgtcctacga
     3781 cacgattggt atttgggcac cgattctgct gttgatctgt aagatggcac aaggtttctc
     3841 ggtcggcggt gaatataccg gggcgtcgat atttgttgcg gaatactccc ctgaccgtaa
     3901 acgtggcttt atgggcagct ggctggactt tggttctatt gccgggtttg tgctgggtgc
     3961 tggcgtagtg gtgttaattt cgaccattgt cggcgaagcg aacttcctcg actggggctg
     4021 gcgtattccg ttctttattg ctctgccgtt agggattatc gggctttacc tgcgccatgc
     4081 gctggaagaa actccggcgt tccagcagca tgtcgataaa ctggaacagg gcgaccgcga
     4141 aggtttgcag gatggcccga aagtctcgtt taaagagatt gccaccaaac actggcgcag
     4201 cctgttgaca tgtattggtc tggtaattgc caccaacgtg acttactaca tgttgctgac
     4261 ctatatgccg agttatttgt cgcataacct gcattactcc gaagaccacg gggtgctgat
     4321 tattatcgcc attatgatcg gtatgctgtt tgtccagccg gtgatgggct tgctgagtga
     4381 ccgttttggc cgtcgtccgt ttgtgctact tggtagtgtt gccctgtttg tgttggcgat
     4441 cccggcgttt attctgatta acagtaacgt catcggcctg atttttgccg ggttactgat
     4501 gctggcggtg atccttaact gctttacggg cgttatggct tctaccttgc cagcgatgtt
     4561 cccgacgcat atccgttaca gcgcgctggc ggcggcattt aatatttcgg tgctggttgc
     4621 cggtctgacg ccaacactgg cggcctggct ggtcgaaagc tcgcagaatc tgatgatgcc
     4681 tgcctattac ctgatggtag tggcggtgat tggtttaatc accggcgtaa ccatgaaaga
     4741 gacggcaaat cgtccgttga aaggtgcgac accggcggcg tcagatatac aggaagcgaa
     4801 ggaaattctc gtcgagcatt acgataatat cgagcagaaa atcgataata ttgaccacga
     4861 gattgccgat ttgcaggcga aacgtacccg cctggtgcag caacatccgc gaattgatga
     4921 ataagctgaa acggatggcc tgatgtgacg ctgtcttatc aggccaattg aactcttaag
     4981 gttcacttaa tctctgacgc gcatactctc ctccaggtta acggaggaga gtgcaatgaa
     5041 aaaccgtgtt tatgaaagtt taactaccgt gttcagcgtg ctggtggtca gcagctttct
     5101 ttatatctgg tttgccacgt tctgatcttt cttcagccgt acccaggccc gcgtgccgga
     5161 agtctcttgc cggttttgca ggaaaaactg cccgtgatgc aactgtgtaa tgcggctgac
     5221 aatacttaac cccagaccaa tcccgccata acggctgtcc atacgtacaa acgctttact
     5281 caactccccg catttactct catcaatacc tggtccttca tcttcaactg ccatgaccgc
     5341 tccgccatct tcttgcagct taatcataat gttgctgcct tgcgggctgt aacgatgggc
     5401 gttttctacc aggtttcgca ataacatccg cagcagggtt gcatcaccct gaacggtgat
     5461 gtcggcggcg ctctctggca atagcagggt ttgctgtcgc tggtcgagca tggtactgag
     5521 ttcgtcatac gaggggagaa tgacatcttc cagcagtttt acatgttgat aattaccgga
     5581 agaaaatgac tgtccggcac gcgccagttg cagcagctgg gagacgctct ccatcatctg
     5641 atcaagccgt gccactaacg gtgctacatc aatgtgatgc gttttcgcca gcagttccag
     5701 atgcaaacgc acccccgcca gtggcgttcg cagttcgtgc gcgacgtcag cggtaaacaa
     5761 cctttcgtta tccagcgtgc tggtcaggcg actgaccaga tcgttcaacg ccgaaaccac
     5821 cgcttcgatt tcgagggtgg cgctgtgaat ggcaatgggc gttaagttgt cggcggtgcg
     5881 cgcttccagc tctttttgca gctccgccag cgggcgggtg atgcggcgta ccgcctgata
     5941 gcagataaat agcgtcaggc tgaccataaa gacgccgggg acaatcaggc tggcgaccgc
     6001 ctcgcggatc tcacgcatga tgtggcgatc gttgttgcga ttgtcgcgta gcgcctgctc
     6061 aaacagctga atctgctcgg tactttcatg ccatagccag aagacgctga tcagctcaaa
     6121 caccaacaaa atggccccga tggtcaatat cagccgttgg cgcagcgata ttggtcggcg
     6181 cagaaaatgc atcagattca attagttttc ctcattcgcg accagcatat agccaaagcc
     6241 gcgcacggtg cggatacggg ctttgcccac tttgtcgcgc agattgtgga tatgcacttc
     6301 cagggtgttg gtcgagggtt cattgtccca gttatagatg tcgttgtaga gaatttcccg
     6361 atgcaccgga ctgcctgctt tgagcattaa ccgtgacagc agagcatatt ctttgggcgt
     6421 cagaatcaac tcttcaccgc ccatccatac ctgacggcga cccatattca gcgtcagatt
     6481 gccaacaatc agctcacttt cgccctgatt attatggcgt cgtagcaggg cgcggatacg
     6541 ggcgtgtaac tcttccagcg caaaaggctt caccagatag tcatcggcac cgacatccag
     6601 cccggcgatt ttgtcggtca gcgtatcgcg agcggtgagg atcagtaccg gcagggtata
     6661 ttttttctgc cggatacggg cgagaaaatg cagtccatct tcgtcgggta accctaaatc
     6721 cagtaccacc aggctgtaat gaccggcctc aaggctttgt tccgccatcc gcgcggttgt
     6781 cacgccatcg cacgcgtagc cttcagtttg cgccgccaga atcaatccct gcaataacag
     6841 cgtatcgtct tcaacaatca gaattttcat tcactcactc tcctgcaagt ttgcagaata
     6901 tcatccgcag cctggtaata cttcgtctca acgccagtta atcccaatag cgtggagaat
     6961 aaattgtctt gtgaatagtg ttgcgtttgc gcctgttttt gcaggcagtt ctggtcaacc
     7021 tgataccgtt tttgataatc ctccgacagc cacagcagca tcggcacctg tttttggcta
     7081 tccggggcga tggcataagg cagaccgtgc agatagatgc cattttcacc taacgattca
     7141 ccgtggtcag aaagataaac caggctggtg gtaaatttat cctgatgttc tttcagcaga
     7201 ttaatcgctt tatcaacaat atagtcgacg taaaccagcg tgttgtcgta agtgttcacc
     7261 agttgctctt tgctacaggt ctggatttca ttggtgtcgc aggttggggt aaatttcctg
     7321 aactgcggcg gatagcggtt gtaataggtc ggaccgtggc tgccgatggt gtgtaagaca
     7381 atcacgccat caccctgcag gttattgatg tactcttcca gcccgtggaa cagtacttcg
     7441 tcatagcatt cgccgttgat gcactgacca ggcagattca gcgcggtgat gttctggtga
     7501 ggcacgcggt cgcaggcacc tttacagccg ccatcgttgt cattccacag cacgttgatg
     7561 cccgctcgct gaatgatatc cagcacgcct tcctggtgct gtgccagctc ttctttgtaa
     7621 tgctcacgcg gcatatccga gaacatgcac ggtactgaaa ctgccgttgc cgtgccgcaa
     7681 gatgcggtat tagggaaata gaccacgtta tctttcgcca gccgcgggtt agtttcacgc
     7741 gggtagccgt tgagggagaa gttctccgcc cgcgaggttt cgccgacaat caggatggtc
     7801 aaatttttac gtttttcgtt ctgcattaac gggttgcggt gggcgtcttc accaattcgc
     7861 accagcggta gatttgccag tcgctgatgg gagtaccatg accagctggc aacaatgctg
     7921 ttagaggggc ttaaggattt caccagctct ttgttattgc ggaacaacga ggcgtagtct
     7981 ttataaaaca gtgcggcgac cagcaaaatc agtagtacag aaaccagaat attggctcca
     8041 cggaaaagaa cactgcgcag acgcgaggtg gcaggtttga tttttatcca gcaggcaatc
     8101 agcgcagcaa gcacgccgct gaatcccagc gttaataaca tttgcggtgt catcagcgca
     8161 taactttctg ccggagtggt atcaataata ttggcaatca tcgagcggtc gatgacgatg
     8221 ccgtaagtca ttatgaaata ttgtgcagcc gcgccaacca gaataaacag gcaggccagt
     8281 ggtcgattaa gccataagaa agagcttagt gtcaggacaa tattaatcac gctgaaagcg
     8341 acgaccggca tcgacaagaa aaccagtacg ttatgcagcg aatccagcgg cagcgcctgc
     8401 aacacctgtt taaaaaaggc aatattcagg cagatagaga tataaaaagc ggccaacaat
     8461 agccaggcga gtaaattcaa agagggtctt tttagtaggc gcttcaacat cacggtgttt
     8521 ccatcgaaca aagtgcgcat atgctcgcaa agcaaaatta agccaacctt aagttcttaa
     8581 ggttggcttt tatgtttgct ggattaaggg gaattaatct ttgcttattg gtacatctaa
     8641 gggatacggg tttttatgca gccggttgta attcagcgca tacatggcgg tgattaccat
     8701 cagggtgaca aatgaccaca taacctcttt cgccccggac cccaccacgg cccaaatgca
     8761 gtagaggaag gcaatggtag taacagccag atatgccggg cgtgctttac caaagtgacc
     8821 gtgaccgagc agcagtaacg ccgcacaggt gtaaagatat ggcaccagtg taaagatgac
     8881 cgagacggaa gaaaccagac cgaactcttt ggtcgcgttt ggtgaaatgc tgctgagctg
     8941 gaagatggtc atcaaaatac cgacgataat cagccccgcc actggcgtgc ccgctttatt
     9001 tacacgggca aaaatcggtg ggaacagtcc gtcatcggca gcggctttcg ccgtttgacc
     9061 cgccagcaac gtccagccgc ccagtgaacc taagcaaccc gcagctgcgc agaaggaaac
     9121 aatggccccg gcggtgtcac ccaacgccat ccgtgcggca tcaccgaatg gcgaagcaga
     9181 aacgcgcagt gcggcattag ggatcatccc cataatcgcg gtggtagaaa gtacatagca
     9241 aacggcggca atcaataccc caccaatggt ggcgataggg acattgcgtt tcgggttttt
     9301 caccacacct gcggcaacgg aggcactttc cacaccgatg aacgaccaca gcgtaacgtt
     9361 aagggtactt tgaattgcac cgaaggtgcc caggccgctg acgttccatg ccgccatata
     9421 ggtttcacca cggaaccaga accagccaaa tacggcaatc ccgacgatgg ggatcagcgc
     9481 cagcacggtg gcaactgcct gcacacgggt gatcattttc ggaccgacaa tgttcagcag
     9541 gacgaagatc cacagcacca cgacgcaggt gatggttaat accaatggat ctttcagaat
     9601 cgggaagaag taacttaaat atcctacgcc aatgaccacc atggcgatat tgccgatcca
     9661 gcaggccagc cagtagagga cgttggtttg ataaccgaga aacgggccaa agcagcggcg
     9721 ggcgtacgcg taagaaccac caggacttgg atcgaggaac gacattttgg cgtataccat
     9781 cgagagcccc agcgcaccga taatcgtcac caaccatcca taaatggcaa tcccgccagt
     9841 agaggccagg tttgcaggta acagaaaaac acctgacccc ataatattcc ccgacaccat
     9901 cagggtgacg gggattaagc ccactttgtg agcatcagca tccgaagaca taattaaact
     9961 cctgcgaagg cgagcttcgt gacaataaat tacgtcatat catacgcctg cattcgatag
    10021 ttgaattatt atcgaccggg ttatcgctga tgccgttatt aatatagctc ttcgttaagg
    10081 ttaaaaatca ggcgacagtg cgttctctgt gctgactaac ataatgcagc ggcgtcatac
    10141 cgtagaagtc tttaaagaca gaaataaagt acgacgtact gttgtagccg caggactgtg
    10201 atacctgaga gatattttta ccgtccatca ttaattcatt tacggcataa cgcatgcggc
    10261 aggtggtgat tatttggcta taactggtgt tttcgctttt caactttttc tttaacagac
    10321 ttgggctaag acataaacaa ctggctacca tactaagatt ccagtctttg tgaatatcgc
    10381 tttcaataat ttgataaacg ctgtcactta cacaattacg taacatatac atcattaatg
    10441 aaaggaattt tttactgtcg agaaaacggg atagcaccgt gaataataat gcgcgtgttc
    10501 gttccttttc tgactcagtt tctgcgggca tcatgctgtg ttgcgcggca acgcgaaaga
    10561 cgtctggcgt caggcatggc agggtgagta tgggcgtagc gctacgttgc catactggta
    10621 tttgcgagag atctttattc agaaaccgga ggtaatcttt gatgatgtcg tgactaatat
    10681 gagcaaccaa agtgttgtta agagaggaga cgtcgataat attattttca caatttaata
    10741 acgccatatg gttagcttta aggctaatag gttctttccc attcaccctt atccagacat
    10801 ctttttcagt caataaaaca atacaaggtt ggtcgctgca aatcctcata catgtactcc
    10861 tgagtgcaaa taaaagacca gttctcgcta aagcaaagcg ataccgggaa atggttatat
    10921 aattgtatga tgaatataaa catgcataaa aataacgagt tttactaata ttacaaattt
    10981 ttatagtaat ttctcatgta catatttttt tacaggataa ataacaggca aaaaaaagcg
    11041 cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt
    11101 tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc
    11161 agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag
    11221 tcccttcagt ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta
    11281 aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga
    11341 ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg
    11401 ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg
    11461 ccatcggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc
    11521 aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt
    11581 gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga
    11641 aggagcaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca
    11701 ggaacataat ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg
    11761 cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg
    11821 gagcaaggat gctgacttta attgggtcga gcatactcca gttatccgga atatctttga
    11881 agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt
    11941 tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt
    12001 tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatat agccgcgcca
    12061 tcgcctgacg gaaatcaacc gcttcgtcga tcacttcctg cgtcagtgac aggccgctgt
    12121 tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg
    12181 tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt
    12241 cacgtacatg aatataagaa gcctgtgaaa gcgcattcag cagtttgtgg gtggagtggg
    12301 tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg
    12361 catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg
    12421 aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc
    12481 agttggtcac cacgcagtaa gacggttttt gcccggcttt gtctttggtc agcgggcttt
    12541 cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa
    12601 tgccgtagcg gttgcggctt ggcaccatat agaccggttt cgcgcctgtc agcatcaaac
    12661 cttgttcgat ggatttatgg cagttacggt caacgaccac gacatcgtta tcggtcatgc
    12721 aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat
    12781 cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa
    12841 gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat
    12901 agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc
    12961 ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg
    13021 gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt
    13081 cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcaga tcccggtcca
    13141 ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct
    13201 catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat
    13261 agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa
    13321 aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa
    13381 cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca
    13441 tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc
    13501 gggctgtaaa gcgcgtgaaa aacaaacatt tttggaatat ttatttttat aaacaatatg
    13561 ttatgtg
//
LOCUS       DAZFRN010000130        13496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFRN010000130 DAZFRN010000000
VERSION     DAZFRN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.132"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            21..851
                     /locus_tag="ACHKUB_003751"
     CDS             21..851
                     /locus_tag="ACHKUB_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO6656712.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(992..1765)
                     /gene="uxuR"
                     /locus_tag="ACHKUB_003752"
     CDS             complement(992..1765)
                     /gene="uxuR"
                     /locus_tag="ACHKUB_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO6656713.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1980..3440)
                     /locus_tag="ACHKUB_003753"
     CDS             complement(1980..3440)
                     /locus_tag="ACHKUB_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO6656714.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3521..4705)
                     /gene="uxuA"
                     /locus_tag="ACHKUB_003754"
     CDS             complement(3521..4705)
                     /gene="uxuA"
                     /locus_tag="ACHKUB_003754"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO6656715.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5045..6388
                     /gene="gntP"
                     /locus_tag="ACHKUB_003755"
     CDS             5045..6388
                     /gene="gntP"
                     /locus_tag="ACHKUB_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO6656716.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISSYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6631..7533)
                     /gene="fimH"
                     /locus_tag="ACHKUB_003756"
     CDS             complement(6631..7533)
                     /gene="fimH"
                     /locus_tag="ACHKUB_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6656717.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7553..8056)
                     /gene="fimG"
                     /locus_tag="ACHKUB_003757"
     CDS             complement(7553..8056)
                     /gene="fimG"
                     /locus_tag="ACHKUB_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO6656718.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNNGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8069..8599)
                     /gene="fimF"
                     /locus_tag="ACHKUB_003758"
     CDS             complement(8069..8599)
                     /gene="fimF"
                     /locus_tag="ACHKUB_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO6656719.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8609..11245)
                     /gene="fimD"
                     /locus_tag="ACHKUB_003759"
     CDS             complement(8609..11245)
                     /gene="fimD"
                     /locus_tag="ACHKUB_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO6656720.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11312..12037)
                     /gene="fimC"
                     /locus_tag="ACHKUB_003760"
     CDS             complement(11312..12037)
                     /gene="fimC"
                     /locus_tag="ACHKUB_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6656721.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12074..12613)
                     /locus_tag="ACHKUB_003761"
     CDS             complement(12074..12613)
                     /locus_tag="ACHKUB_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656722.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12678..13226)
                     /gene="fimA"
                     /locus_tag="ACHKUB_003762"
     CDS             complement(12678..13226)
                     /gene="fimA"
                     /locus_tag="ACHKUB_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6656723.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3267 a         3485 c         3279 g         3465 t
ORIGIN      
        1 actcctaaaa ggtaactatt atgtctatgc cattaagcaa tgcccttcag agtcaaatta
       61 ttacagataa tcacttcctt catcacccaa aagtcgacaa tgagttaaca cgtaaatatg
      121 aacgagctcg cctggatacg gaaaatatct atcttctgcc tcttgctcgt ggcaataacc
      181 ataattacga tggaaaaagc gttgtcgaaa ttagaaaact tgatattagt aaagagtcct
      241 ggccatttaa ttatgtgaca gaagcatgca gagaatccga tggtattaca acaacaggac
      301 gtatgctcta tcgaaacttg aaaatcacct cagctttaga cgaaatatat ggtggtatat
      361 gtaaaaaggc gcatgctgct acagaattag cagaaggttt acggctaaat ttatttatga
      421 agagcccttt cgatcctgtt gaggactata cagttcatga aataacatta ggccctggtt
      481 gcaatgttcc aggttacgcc ggaaccacga taggatatat ttcaaccctt ccagtttctc
      541 aggcaaaaag atggaccaat gagcagccaa gaattgatat ctatattgat cagataataa
      601 ctgtctcagg ggtagcaaat tcttccggat tcgcccttgc cgcattactt aatgctaata
      661 ttggaatggg aaatgaccca ataattggta tagaagccta tcccggtacc gcggagatcc
      721 atgcaaaaat gggatataac gttatccccg gtgatgaaga tgcgccactc aaacgcatga
      781 ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa tggaattata
      841 ttggtaaata gccactgaga agaatgagta ctaatattca ggttatacag atatttaaac
      901 aataccctgc aatttacaaa gtaatactca ttgcgacttc ttaatttaag aaagcgcaag
      961 gaacgtttac ccttgcgctt attataataa gtcaggcgtc gactttatcc agcggccatg
     1021 aatcaaacag atagccgtca aaataaatat cgtcaacgtt cgagaattcc agcagacgtt
     1081 gcttaacgtt ttccagatgc tgccacattg ccagcttcgc cgctcgggca tcttttttga
     1141 ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc tttgcgatac aggctgtcat
     1201 ccagatggct gtgcaactgg atccacattg ggttgttttc ccgccactgc caggactgac
     1261 ggaacagctc caccagcatg ctgttatgcg ttgcttcggc aatagcgaga tggaactgca
     1321 tgtcaccgct ttcgctgctg cccggcgcac tggaagccag ttcacgctct tccagttgca
     1381 atgcctgacg cattttgacg atatcttcgc gggtagcctg cagggcggca aactcggcga
     1441 tgttactttc cagtaactga cgcgcctgta acagctcaaa aggacccgca tcattgcaga
     1501 cgttggcatc cggactgtct gtgttgtggc tgcctgagct atcaagaaca tagataccgg
     1561 caccccggcg cacttccacc agccctttga tctccagcat gatcagcgct tcacgtacca
     1621 ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc cggcggcagc cgttcgccag
     1681 ggttgtacgg cgtctttatg atcagatcgc ggatcatcgc cccgacttcc tggtaaggtc
     1741 tttgcgcaga ggtggcagat ttcatggtgt tgtgtccgtt aggtgcggtt aatcaaattt
     1801 ttgccggatg cggcgtgaac gccttatccg gcctacaacg ggcaatttta cggcccccgt
     1861 taatctcaaa ctataccatt agcactggaa gttggcacgt acaggtgggc aaaaaaacga
     1921 aaactggtca accaaatatc agtttgattg accagtaagt ctgtagggta atcagttagt
     1981 tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca gctgctgata tgccgccgtc
     2041 actgcgccaa caaagtcggc attctgcggc agatcatcgg caaaaatacc gctcaggccc
     2101 agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt tgatcttctg gaactccgcc
     2161 agcatcgggt cgaccacgtc aatggcatta ccctgctcat ccacgccctg ggtgtaacgc
     2221 atccagccag ccacgcccag cgccaggtga cgccagctgc cgccgttttg caggtgcaga
     2281 cgcaccgggt ccagcagacg ctgcggtaac ttctggctgc cgtccatcgc aatctgccag
     2341 gtacggtgac gcagagacgg gttgctgaaa cgctcgatca gcagcgtcgc ataggcgttc
     2401 aggtctgtac cttccggcat cgacagcgtt ggcgcttgtt cctgcatcat cagggcaaag
     2461 gccgctttgc gataagccgg gttagtcatg gtgtcggcaa tggtttcata gccgccgagg
     2521 taaccgaggt acgccagaaa agagtggctg ccgttcagca tacgcagctt catcatttcg
     2581 aacggcacaa cgtctgcaac gaactgtgcg cccactttat cccagtccgg gcgaccatta
     2641 acgaagttat cttcaatcac ccactgacgg aacggttcgc aggcaatggc gcacgggtcg
     2701 taaacaccca gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc cggaacgatg
     2761 cggtcaacca tggtgcacgg gaaggtgaca ttttcttcaa tccatgccgc cagctgcggg
     2821 tcacgcgcct gagccagccc cagtaccgcg accttcgcca catgtccgtt ttcacgcacg
     2881 ttatcgcagg acatcaccgt aaacgctttc aacccttttt cacgacgcag acgcaaggct
     2941 tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt tttccagatc gtgcttgatc
     3001 agcgggttat tgagatccag ttgaccgctt gccgcatcag cgcagtagcc tttttccgtg
     3061 accgttagag agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat accttcgcag
     3121 ccatcgattt ccggatgcag cgcttctttc atcgaaccga taattttcag ctcggtgctc
     3181 tctgcgcctt tttccgctac ggtgtacagc agttgctgtt ttttcaggtt ttcgatcagc
     3241 acgcggtcgt tgcctggcat caggttaact tcgcagatgc cccagtcgct gtcggtgctt
     3301 tccagcagat gatgggtata cagcgcctgg tgcgcgcggt gaaacgcccc gcaaccgaga
     3361 tgcacaatgc gtgattccag acgagaatga tcccatgacg ggcgggcaac cggcagattg
     3421 ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc ttcagagagt gacggcaagg
     3481 aagaaggaac cggcacggcg cgcagccatg ccggtggata ttaacggcta aagaaagcgc
     3541 gctggatcgc cagttcgaca ccgcgaactt cggccaggcc tttcagacga ccaattgcgg
     3601 agtaacctgg gttggttttc ttcttcaggt cgtccagcat ctgatgaccg tggtccggac
     3661 gcatcgggat caggtcttct ttgccttccg ctttacgacg gtgttcttct tcaacaatcg
     3721 ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg tggaaggttt
     3781 tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc ggaccgaact
     3841 gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg gtgcacatgg
     3901 taaaaccgtt tgccatgctg tttacggtat caaccatcca ctgcatatct tcaatggtgg
     3961 aaacaatgcg cggcaggccg aggatcgggc gcggcggatc gtccgggtga acagccatac
     4021 gcacgccgac ttcttcagca actggaataa tcgctttcag gaagacggca aagttttcgc
     4081 gcagcttcgc tttgtcgata tctttgtaca gctccaggtg tttacggaac tggtcgaggg
     4141 tgtagccttc ttccgcgccc ggaagaccag caatgatgtt acgggtcaga cgcgctttgt
     4201 cttcgtcgct catagtggcg aagcgaacag cagcctgagc aatttcttct tcggtgtaat
     4261 ccgcttccgc gcctggacgt ttcaggatat gcatttcgaa tgcagcgaat tcgatctggt
     4321 cgaagcgcag cgctttggag ccgtctggca gcacgtattc gaggtcagta cgggtccagt
     4381 cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc gcactgcgcc aggttgcgca
     4441 gagtctgctg atagttagcg atccactgct catagttgcc agtgtgggtt ttgatatctt
     4501 cgtgaattgg tacgctttct acgacagacc acaccaggcc tgcgtcttca acgatcgcct
     4561 tgcgtttgag gatctcttct acggaccata cttcgccgtt cgggatatgg tgcagcgcgg
     4621 taaccacgcc agttgcgccc gcctgacgga catcagctaa agaaaccgga tcgtttgggc
     4681 cgtaccagcg ccaggtctgt tccatgattt ttcctctttc acatagtggg ttccacagca
     4741 aagagacgcg gaaccctgga gttgtttagg tatccggatg aacaaccaga ccgcagccgt
     4801 ggcgttgtat cggatgagat ggcgtataag ttctaccttt tggtaagaca tgtcaacccg
     4861 aattctgaaa ttggttaacc acatcacaag aatttcactc tcatggttgt tgctgcaaat
     4921 tgcagcaaag ggtaatcgat ggttattgat cagaggatgt gacattcatc gcaacaatgg
     4981 ttgaccaatt tacataacat gtcgcccaaa ataacaacgg ttcaaccagg taaggtaaaa
     5041 cgttatgcat gtgcttaaca ttctctgggt ggtattcggc attggcctga tgctggtact
     5101 gaatttgaag ttcaaaatca attcaatggt ggctttgttg gtggcggcgc tgtccgtcgg
     5161 gatgctggcg ggcatggatt tgatgtcgct gctgcacact atgaaagcgg gcttcggcaa
     5221 cacgctgggg gaactggcta tcatcgtggt gttcggtgcg gtcatcggta aattgatggt
     5281 cgactccggc gcggctcacc agatagcgca tacgctgctg gcgcgtctcg gtctgcgcta
     5341 tgtacagctg tcggtgatta tcatcggcct gatttttggt ctggcgatgt tctatgaagt
     5401 ggcctttatc atgttagcgc cgctggttat tgttattgcc gccgaagcta aaattccgtt
     5461 cctgaaactg gcgatcccgg cagtagcagc tgccactacc gcacattcac tgttcccacc
     5521 gcagccgggt ccggtggcgc tggtgaatgc ttatggcgcg gatatgggga tggtttatat
     5581 ctatggcgta ctggtgacga tcccaagtgt aatctgcgca ggtctgatcc tgccgaagtt
     5641 cctcggcaat cttgagcgcc caacgccatc attcctgaaa gcagatcaac cggtagatat
     5701 gaataatctg ccctctttcg gcgtttcgat tctggtgccg ctgatcccgg cgatcattat
     5761 gatctctacc accatcgcca atatctggct ggtaaaagat acccctgcct gggaagtggt
     5821 taactttatc ggttcctcgc cgattgcaat gtttattgcg atggtggttg cattcgtact
     5881 ctttggcacc gcacgtggtc atgacatgca gtgggtgatg aacgcttttg aaagcgcggt
     5941 gaagagcatt gcaatggtga ttctgatcat cggtgcgggt ggcgtgctga agcagaccat
     6001 catcgacacc ggcattggcg acaccatcgg catgttgatg tcccacggca atatctcgtc
     6061 ctacatcatg gcatggctga tcactgtgct aattcgtctg gcgacgggtc agggtgtcgt
     6121 ttcggcgatg accgccgccg ggattatcag tgctgcaatc cttgatccag caactggtca
     6181 gctggttggc gtgaatccgg cgctgctggt actggcgacg gctgcgggtt ccaacaccct
     6241 cacccacatt aacgatgcat ctttctggtt gttcaaaggt tactttgacc tgtcagtaaa
     6301 agacacgttg aaaacctggg gtctactgga gctggtcaac tccgtggttg ggcttattat
     6361 tgtgctgatt attagcatgg tagcgtaaaa ggacaccaga gcctgccaat ggcaggctca
     6421 gactgatgac aaagtctaaa aatgtgccca gacagtcccc tcgcccctcc ggggtgaggg
     6481 ttagggtgag gggaaaagac ccgcaaagtg cgctttgttt atgccagatg cgacgctgac
     6541 gcgtcttatc tggcctacaa agggctaacg tgcaggtttt tagcttcagg taatattgcg
     6601 tacctgcatt agcaatgccc tgtgatttct ttattgataa acaaaagtca cgccaataat
     6661 cgattgcaca ttccctgcag tcacctgccc tccggtacga gcgtaatttg ccgttaatcc
     6721 cagacttacc gccgaagtcc ctactgctcc taacgatacc gtgttattcg ctggaataat
     6781 cgtaccgttg cgcgtcaact gtacgccgac gccctgcgct ggtgaaaacg aggcggtatt
     6841 ggtgaaaatc gagttgcccg catctgcggt tgtgccggag aggtaatatc ccaggttttg
     6901 gcttttcgca caataaacgg taagaggaat tggcactgaa ccagggtagt ccggcagagt
     6961 aacggtgaca tcacgagcag aaacatcaca gccgccagta ggcaccacca catcattatt
     7021 ggcgtaaata ttccacacaa actggaaatc atcgctgtta tagttgttgg tctgtcgcaa
     7081 aataagcacg gcaattaatg agccagcttt aatcgccacc ccgcccgcac tgctcacagg
     7141 cgtcaaataa agcgccaccg gccacggctt atccgttctc gaattataaa caacgcgcgg
     7201 cgtttcgctg gtggtcggaa atggatagct actgccacta tattttacgg tcccggaaaa
     7261 attagataac acgccgccat aagccgagcc tcgttgcagt gtgacatagt ctgtaatggt
     7321 ttccggataa tcgttatggc aaaagatttg cgtcgaaaga tctacgacca ggttttgccc
     7381 cacattcacg gcaggcgcaa ggtttacata aacattagcg ctgccaccgc caatagggat
     7441 agcggtacca ttggcggttt tacaggcgaa tgaccaggca tttaccgacc agcccatcag
     7501 cagtacagca aacagggtaa taactcgttt cattacaatc atctcttcgg gttcagctgt
     7561 aggtataggt gatgctaatc actgcctgaa tggttccctg agtggctccg ccatttactg
     7621 tcaatgctct gacctgtaac gggaagtgcg ctgattgtga ggaatcatcc acctgaactg
     7681 ttttggttgc gccattattc aatgtgttgc cactgtcatc ctgtagctct aactggatgt
     7741 tttgcgcggt cccctggttt ttataatatc cggtactgtc ggctgccccg ctgaagctgg
     7801 cagtgaccct cgacgttccc accggacaat tagtcaactc aagcgcaaca tcatgccagg
     7861 ccgatgccgc cccggcagac ataagactga aagaataaag atcgccgaga tcaaccgtgg
     7921 cattggtggt ggaaactgtg cacggtttgg cgacgacctt accgttcacc gtgatggtga
     7981 catcggctgc ctgtatcgtc gcacttgcga gcgccaatat tgccgccaat aaatacccac
     8041 gtttgcgcca tttcatgagc atctccagtt actgatattc aagagtgaag gtagccgtgg
     8101 cattgatatg ccccgcagtg actggcacct gtgtcgccat tagccgggcg taaaaattca
     8161 gcgtatttgg tttacccggc gtcagggtcg tccacgaaat cgcggacgat ggagcattaa
     8221 ggggtatttg attttgctgc tcattcagaa gctgtattcc cagtcccgca gccgctgaca
     8281 ccgtattttc aagtgcaagc aggttggcat tgtggctatc tgcaacgcca gtaaacccaa
     8341 cctttacggc agaaacggcg ttaccacagg gtgacagcaa aatacgaaat ggaacgacag
     8401 gagtcgtcgc gccaatgttg ttaaattgct tcgccgcgtt ttccatcaga tcaacagtaa
     8461 aattggttga ttcagcggcc acactacagc cgttatctct gacatagccg cggatagtaa
     8521 tcgtgctatc cgcagccaaa gcgtgactca ccgccagcca caaaaaagcg cacagaagat
     8581 aaaaaggttt gtttctcatc acgccccctt aacgacattc agctgatagc tgggttaata
     8641 actgctgctg actctctggt ggcagttgat aattggcgac acagtgagca ttttcctctt
     8701 ctccccattt cacctgaact tttcccgcta aaggcattcc gctgaggtaa acctgaccat
     8761 tatccgcaac aatgccgcta ctctggctac tctctgatgt caccatcgcc ccaaacggca
     8821 gcggcttatt attgtgggtt agcgtcatga gcagttttat cccaacgcgc gctttaaact
     8881 ctgctcgcac gatcgcccca cgagtgggaa caacgttagc gaccgcgtta tctaaatcga
     8941 cgttatcagc cagggtattg gtatccagcg ccactctgtt ttcccgatat tcagtggcat
     9001 aaggcagcac ggcataacca cgccagtcgg tacgcacccc cgtctggttt tcgacttttg
     9061 catcttttgc gccaggcgct ttaacaagca ccactgtttc gtttaacggc tgccccagcg
     9121 ttacgccatt ggcatgagcc aataccccac cgctgactcc gtaatagagc tgcttaatat
     9181 catcgctatg gctgtaaccg atattggcat tgccgtaacc accgcgataa ttcagcgtgg
     9241 cgtagcctgt gcttccgcta ttaccatcgc ctcccccggc atagccggtt tgcacgctat
     9301 agctgaggtt gttgtcttcc agcaacgtac cgtatacgcc agccagattg gtcatccgac
     9361 cgttgagatc gtgtgacatg ctgtagctgg cactggcatg tcgccactga gatttactgt
     9421 cagaacgcag ccagtggctg aaaggaatat tgacgttaag cgctaacatc tgatcccgtc
     9481 ctttttgcca ggcatttttc gtcaggctat agctgagcgt ccagttgata tcttcgaatg
     9541 cagtatttaa accagcctgg aattgctcat cgacattact cgttccccaa taagtttgat
     9601 ggctaccact caaatacagt gttgatgtgc gcccgagttg ctgagtaacg gtgagctgta
     9661 atttcccgcg tttgttataa gcgaggttgt aatagtcggt gaatttcggc ttaacctgaa
     9721 taactccgtc ctgtgtttcg atgttgtagc cattcattcg actgtatgtt gtatcagcga
     9781 aattaaaata tccgctggtc gaataacggt aacccactaa ctgaatattc gtgcctgact
     9841 cattgagcga tttgttatag agaaaacgca ccgattgtcc gtcatgctga ctgtcatcgg
     9901 gaagtgtgga attagcctgc gtcatatcca cagacagagc gcccagtgcc cccatgtttt
     9961 tcccgatacc gaaattaaaa gcacgataac gatccgccag ttgcgttcca ccatatattg
    10021 tccagccagc cggaaggccg tggagtaatg tactctggaa aaagcggggt ttttcctgct
    10081 gcgcatttcc actacggtat tctcctgccg taatggaata acgagtatgc ccttcacgtt
    10141 gcaaaagcgg gactgacgaa tagggtacgg taaaaatctg cgtgctgccg tcagcctctt
    10201 tgatcgttac ctgcaagtca ccactattac ctgcggcata gatatcgttg atggtaaaag
    10261 gccccggtgg caccgtacta ttataaatgt catacccatt ttgtttaata gtgacctgtg
    10321 cagtaccacg agcaataccg tggatcaccg gggcaaatcc tctttggcta tcgggtaaca
    10381 tattgtcatc tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa atatcgccct
    10441 gagtataacc atcacccagc gtcagccggg aacgtaacgg tattatgtct cgctcaagcc
    10501 aggtattgat atgctgccat ttatttttgc tacctgatga tctgtcgcta ctgttataac
    10561 tccaggtggt attgtcgcgt aaacgccacg caccaatatt taacccactc tgtaggttta
    10621 aatatgcata atggctgtta cccccaatcc gattctgtac actatttccg ctgaaattat
    10681 aattgagcaa tccggcatta ataccgggat cccataactc aggaggaata taaccacgcg
    10741 cgcgattact cataaatgcc tgagggatcg tcaggttgag tcgctgctga ccaacatcta
    10801 aatgcgcagt agcgtcatgg atcattgagg ttaatggcac gcaggcatca tccgccagca
    10861 gattcatacc ggagacagaa gccgtattca gccccatact ggcaagttgc gcgcgtgtca
    10921 ggcagggaac aatcccttgt tcactgtcgc ccgtattaaa tgtgacatca cgcgttgcca
    10981 tataaccatt attcaaatag atatcgacac gatacgtccc tggcggtaat tcttgcccat
    11041 tttcaaaacg cgataaatcg gccacagcct ggggatcatc cgctaaaaag cgcggattaa
    11101 aatagagttc ggcagatgac aaaggtgcct gtgcggcaaa agcacaggcg acaacgagcc
    11161 ggacaaaaaa accagccaaa cgatgcttac gaatatgcaa gcattgtgtg tttcgctggt
    11221 aaagtcttaa attcagatat gacattattc ctccggttta aaatcacccg gcagtcaatt
    11281 ctttttattc aggcgaaaaa ttccccctgc gttattccat tacgcccgtc attttggggg
    11341 taagtgcgcc ataatcattt attgttcggt aagtaatatt gcttcctgca tcagaaggca
    11401 atttaaccgt gctttcgccc attggaggca ccaatgcatt ttcaagaacc cgggttccgg
    11461 cattcaactc tgttaccgtc aggtaatagg gtgtcgggtt aatcagcgtc agggaattcg
    11521 cgctacgacg aaatcttaat ttttctgcgg cctgatcggg tggcaacgct aatttagccg
    11581 gacgatagta cagtttaatg cggctgataa ttgcgagctg tagcgtattc tcagtcaatt
    11641 ttgatttatc cattgacgga atcgctttaa cattcatcca gaataaactt tcccgatcct
    11701 gtggcaattg gttattagtt gcatcaagaa tacgcaaggt attctctctt tttcccttca
    11761 tcgcaaacag aggaggcgtc acgataaaac gaccatcctt tacaccatcg gcattttcca
    11821 cccatgattg aattaaatag gtactatttt catcattatt tgtcacggca agttgcactt
    11881 gtttttgccc tgccggataa attacgcgag tcgcacctaa ggccactccc gcttcagcgc
    11941 gtccggcaac catcattgcc atgaacatca ggatacctgc cagcaagcag aatgttattt
    12001 cctgcgattt ccttacattg acgtttttat tactcactgt cctgttcctg ttatcacatt
    12061 atctgctgaa caattactga taggttaaag agaaccaggc ctgggcattg gcgatgccgc
    12121 cagtaacccg acgcccggta gcacgatatt tggcgatgaa atgtagcgaa gtagagcctg
    12181 agtaaagccg tttccagttt gctggaggac gattaatcgg tacgaggttt ccttcatcat
    12241 caaacaacgc tacgccaata ttggtggcta tccctggccc ctctcccacg gaaagcacat
    12301 ccggattttt accatccgcg acaccgtgaa acgccacacc cacacgttca ctcaccaccg
    12361 tgctacattc ccgtaaatga ataacaaaag gcaccggtgc gctatcttcc cctaccgcat
    12421 gaaaccggtt actgctgatt tgccccatat tgaccgtcat ttgtttatca ccggcttcaa
    12481 tccggcaagt ttccgcaata atgacgccct gaaattgcat atttccgccg ggcagcgtgg
    12541 tattccattt atttccggcc agagcaaaca ttggcagcaa cgacgctaat agaaataatc
    12601 tcttcctttt catcgttatt tttatcgcac aagggtgggc atccctgccc gtaatgacgt
    12661 ccctgaacct gggtaggtta ttgatactga accttgaagg tcgcatccgc attagcagca
    12721 cccggggttg cggcaccggt tgcaaaataa cgcgcctgga acggaatggt gttggttccg
    12781 ttattcaggg ttgtttctga actaaatgtc gcaccgtcca gcgccagcgc agcacccgtt
    12841 ctgtccagga tctgcacacc aacgtttgtt gcgctacccg cagctgaact ctgcagagcc
    12901 agtacatcag tatgcgtagc atcaattgcc gtacctaaga aggcaacagc ggcttttgtg
    12961 gcaacagtgg tatcgcaatc attcagctga atgttaaaac caacggcaga gctggttgct
    13021 ccagcctgct tcaggctagc ggtacgaacc tggcccaact gaacggtttg atcaacagag
    13081 cctgcatcaa ctgcgcaagc ggcgttaaca acttcccctt taaagtgaac ggtcccacca
    13141 tttaccgtcg tagtatcggc cagagccgct gcggaactga gggacagagc cgacagaaca
    13201 acgattgcca gagttttaat tttcatgctg ctttccttta aaaaactatt tctaaatcga
    13261 catgggcagt cgttctgtac actttgtttt gtcaacgagt ttgactgcca acactgcaca
    13321 gttttccccc aaaagatgaa acatttgggg ccattttgac tcatagagga aagcatcgcg
    13381 cacaaacttt ttcagtttat ttgttggctt aatattctat tgttatcttt atttatagat
    13441 ttttatattg catgaggtgg tttttggaga gaagaatgag gaagttgcgt cgagcc
//
LOCUS       DAZFRN010000131        13352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFRN010000131 DAZFRN010000000
VERSION     DAZFRN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.133"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            104..460
                     /locus_tag="ACHKUB_003763"
     CDS             104..460
                     /locus_tag="ACHKUB_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6656724.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(511..1407)
                     /gene="yhaJ"
                     /locus_tag="ACHKUB_003764"
     CDS             complement(511..1407)
                     /gene="yhaJ"
                     /locus_tag="ACHKUB_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO6656725.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1512..2213
                     /locus_tag="ACHKUB_003765"
     CDS             1512..2213
                     /locus_tag="ACHKUB_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO6656726.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2236..2400
                     /gene="yhaL"
                     /locus_tag="ACHKUB_003766"
     CDS             2236..2400
                     /gene="yhaL"
                     /locus_tag="ACHKUB_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO6656727.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2612..3922)
                     /gene="cyuA"
                     /locus_tag="ACHKUB_003767"
     CDS             complement(2612..3922)
                     /gene="cyuA"
                     /locus_tag="ACHKUB_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO6656728.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3950..5281)
                     /locus_tag="ACHKUB_003768"
     CDS             complement(3950..5281)
                     /locus_tag="ACHKUB_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6656729.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5557..6921)
                     /gene="tdcG"
                     /locus_tag="ACHKUB_003769"
     CDS             complement(5557..6921)
                     /gene="tdcG"
                     /locus_tag="ACHKUB_003769"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6656730.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6993..7382)
                     /locus_tag="ACHKUB_003770"
     CDS             complement(6993..7382)
                     /locus_tag="ACHKUB_003770"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO6656731.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7396..9690)
                     /gene="tdcE"
                     /locus_tag="ACHKUB_003771"
     CDS             complement(7396..9690)
                     /gene="tdcE"
                     /locus_tag="ACHKUB_003771"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO6656732.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREIDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9724..10932)
                     /gene="tdcD"
                     /locus_tag="ACHKUB_003772"
     CDS             complement(9724..10932)
                     /gene="tdcD"
                     /locus_tag="ACHKUB_003772"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO6656733.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10958..12289)
                     /gene="tdcC"
                     /locus_tag="ACHKUB_003773"
     CDS             complement(10958..12289)
                     /gene="tdcC"
                     /locus_tag="ACHKUB_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO6656734.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12311..13300)
                     /gene="tdcB"
                     /locus_tag="ACHKUB_003774"
     CDS             complement(12311..13300)
                     /gene="tdcB"
                     /locus_tag="ACHKUB_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO6656735.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3412 a         3627 c         3166 g         3147 t
ORIGIN      
        1 taaatatcaa aacctttata acatccgaaa aagaaagata cgaatagcat ttacacaata
       61 cccggaatag actttcgtca cttttaatta aagggatgtt tttatgcagt ggtatttagc
      121 cgtattaaaa aattatgttg gtttctctgg tcgcgcacgt cgtaaagagt actggatgtt
      181 tactctgatt aacgccatcg tcggcgctat tatcaatgtc attcaattga ttttaggttt
      241 agagtttcca ttcctttctc tcatttacct ggcagcaaca atcattccgg tcattgctct
      301 gtgtgttcgt cgtctgcacg atactgaccg ttcaggtgcg tgggcgcttc tctatctggt
      361 accgatcatt ggctggttag ttctgtttgt atttgcctgt ttagaaggca attctggcag
      421 caatcgttac ggaaacgatc ctaagtttgg ttcaaattaa tcttagaatt ggggcgacat
      481 ttcgcccctt tttattaata ataacaaata ttattttccg ctaaaaagtt tgggaatttc
      541 ccgcagacac caggatttcg cttcccccat actgtcacga cgccaggcca taataatatc
      601 gatctcgctg gtcgattccg ggctgacgac acgcaaccgc ccttccgcaa tatctttttc
      661 gaccatcgga tacggcatcg tcgccacgcc aagccccgcc agtaatgcct gacgtttatc
      721 ttcaatcgtg cttaccgtta agcgcggctg tttgtccagc agctgtacgg tcaacaccgg
      781 gcgctcacga gcggtatccg ctaccgcaat tccacgatat ttcacgcgcg tcacttcaga
      841 taacggttcc ggctcctgat gaatcgggtg atcaggcgcg gcaacgtaga cgttcattaa
      901 cgtatagagc ttgcgcgagt tgatctccga cgaggaacga aaatgcatat ccggcgcgat
      961 aacgatatcc gcccgcccct gctccagtcg ttcccacgcc cccgccagca cttcggtgat
     1021 gattgccagt tgggtattgg cttttgccgc cagtttgtcg attaacggga aaaaggcagg
     1081 tgtcggtacc agcgcctcgg tcacaatggt gagatgcgtt tcccaaccgc gcgcgagggc
     1141 ttccgcatcg gtagtcagtt tatctgcggc ttccagcaaa acgcgccctc gctccagcaa
     1201 catcctcccg acattggtga atttggtgcg atggcccgag cggtcaaaca gcaccacatc
     1261 cagctcttct tccagttttt gcatggtgta gctaagtgcg gaaggcacgc gtcccagctc
     1321 atccgccgcc gccgcaaaac tgccccggcg atcgatcgca tccataaccc gtagtgcttc
     1381 cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg aacataccgg
     1441 gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga agtaaagaga
     1501 ggtcaagaat tatgattact acccgaactg ccagacagtg tggacaagca gactacggat
     1561 ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa ttgttaggct
     1621 atgcctccct gcgtgtgctt aaccaggaag tgctggcccc aggtgccgcc tttcagccgc
     1681 gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagca gagtatcgcg
     1741 acagcgaagg caatcatgtt caggccagcg ccggtgaggc gttgctgctc tctacccagc
     1801 cgggtgtcag ctacagcgaa cacaatctca gcaaagacaa accgttaaca cgaatgcagc
     1861 tttggctgga cgcctgcccg cagcgagaga atccgctgat tcaaaaactg gcgcttaata
     1921 tgggcaagca gcaattaatc gcctcgccag agggtacgat gggaagcctg caattacgcc
     1981 agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat ttccagttgc
     2041 atgggccacg cgcgtatttg caatcgattc acgggaaatt tcatgcgctt acgcatcatg
     2101 aagagaaagc ggcgctgacc tgcggtgatg gggcgtttat tcgtgacgag gctaacatta
     2161 cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc tagttgtttt
     2221 tacagggaga tgatgatgag taaaaaatcg gccaaaaagc gccagccagt gaagcccgtg
     2281 gtggcgaaag aacctgctcg caccgccaaa aattttggct atgaagagat gttgagcgag
     2341 ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc taccgcgtaa
     2401 agacaggttg ccggatgcgg cgaaaacgcc ttatccggcc tacaaaatcc aacaacatta
     2461 acgaattaaa caacccgtag gccggataag atgcgccagc atcgcatccg gcatcaggta
     2521 caaaacacaa caacatcaac aagttgagca ccccgtaggc cggataaggt gcgccagcat
     2581 cgcatccggc acgataccca aaacctggcg tttatctggc cttgctcgcc ataatctcga
     2641 taatctgccg atccgtttgc tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg
     2701 actgctcaac atcatgcgcc acgatccctt cattgccggt cacggcggta tcatccagcg
     2761 ccattaacac cgctttccac gcagccgaag cactggtcga aaccttcatc gcgcagctgt
     2821 tcgacgcacc atcgcaaatc atgccgctga catcgccgat catactgctg atcgccatcg
     2881 agatggtttc ataacgccca tccaccagcc atgccatccc ggcggcggcc cccattgctg
     2941 cggtcgttgc ggcacacagc gcagacaaac gcggtaactg gttatggatg taaattgcgc
     3001 tcaaatgaga aagcatcagc gcacgcgcca gccgttcatc atccgctccg aagtgttctg
     3061 ctaccaccac cacaggcatt gttgcggtga tcccctggtt acccgagccg gagttactca
     3121 tagccggaag cgtagcgccg cccatacgcg catcggatgc cgcgctggta cgaatcacaa
     3181 tggatgaaga gagatctttc gccagcaaac cgcgctcgca ctgtttttcc agcgtcgcgc
     3241 caatatgcag cccccactta ccgctcaaac cttcctgcga taacgcacaa tttagcttcg
     3301 cggaatcgag aataaagcgg atcgccgcaa acgggacttc attgacgaac ttcaggatct
     3361 cagccagcgt cgttctggaa agcaccgtca gcggagactc ttgctcgccc tctgccacac
     3421 acgcctgctg ggtaaacacc acaccattgt gcgtctcaat atgcacaatg ttggtatgcc
     3481 cgccgacgat ggtgacacac gcccacttct caccgttcca gactttggcg cgtgagaaga
     3541 ggatttcatt gcaaggttcc tggatcttaa cggagacttt ccccgccgcc agcagtgctt
     3601 tggcatcggc aattgcctgc gcagttgcgt ctttcagcac ttccagcccg gcgttggcat
     3661 ttccacctaa cgcccccagc gccgccgcaa tcggcagccc caccattccc gtgccgggaa
     3721 cggtgacgcc cagaccgttc ttcatcagat ttggcgaaac ccaggcttct acacgttcaa
     3781 ccggaccttc cagttctgcc gcagcaaccg ccgccgccag cgccagtgaa atcggttcag
     3841 tacatcccag cgccggtttt acttcctcct gaacggcgag gatgtaacgc tgccataacg
     3901 gatttaaagt cgaatcaaac atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg
     3961 ccaggaacgg agaaacacaa agcaacaaac cagtgacgat aatcaggtac agagacatcc
     4021 ctttgtattt gtgcaatgcc ggtactttgt aaaccagcca cgccgggatc aggcacccta
     4081 ccatgccgaa aatcgggcta cagatagagg tgaagctcaa caccggtgcg ttcagtacga
     4141 tggcgctcca ggccagcaaa atggcgaaaa tcatgatgcc gcgctgaaca agattttcgt
     4201 taatcttctc ggcaggcatc ttgcgacgca ggatgttcat tacgatccct tgcgttgctt
     4261 cgcgaaagcc taaatagacg ccaaagaatg cagtcatgac agcaaagata ttgagaatga
     4321 cgctgaccac tttcacccac gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca
     4381 gcgcggaaat attctgctca taggctttta ccgcttcgtc atgtcccatc gccagcgtga
     4441 acgacacggc gtagaaaaag acggtgacaa acaaaatgcc aaacgcgata ttcattgccc
     4501 gcaatgcttt atgccgcgcc acttcaattg atttttcccg cgagcgataa gagatcacca
     4561 tcgggcttaa cgtctggata aacagaatcg acgtcagggt aaacggcagc gtaataatgg
     4621 cgtttttcac cagcagcccc agcggcggta gcgaaccgac gttatacaga tgccacattc
     4681 ccaccatcga cacgcccagc gccgcgacca ccagcagctt ggtcagcacc atgccggtcg
     4741 aaattttgaa taacaatttc tcgccgcgtg aagagatcgc caccagaatg caaatcagta
     4801 ccagaccata aaagggactg tctgacagca acccttccgt cacgccgaag gtatgcagat
     4861 aggaagcact atcgttggtg atggcggtgg aatagacgaa catccaaatc accagcatta
     4921 cgaaatagag cgcacctaac aggatgcccc agtttttacc taaataaccg ctaatgacac
     4981 tcgggtaatc tttacattct ggtgattctg ccagcgtatt aataaacaac cgctgaaaca
     5041 gatacattgc cgggtaacca atcaccgatg agagcaaaaa tacccacaat cccatcaaac
     5101 cgacctgcac cgggagaaaa acaatccccg cgccaatcgc catcccgata ctcataatca
     5161 cccagccggt gtcagtactg tcgaatttga tcgcttctcg ccactcgctc tcactcattc
     5221 cagcacgacc cgccggggtc gaagcgtctg caatgacgcc tttattcgat gcaatttcca
     5281 taatttctcg ctcaatattt tgtagggctt attatttttt tccgagccgc atcaaggcga
     5341 tatgcggtct acgtttttgc aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc
     5401 agagaaaatc ttcacaatca agactttgaa ataacggaga tggataagaa ttttctacta
     5461 aattaatcgc aagagaatat tcattctcta tttgcggcag atcacaaaaa aaaaggtgca
     5521 catttgtgca cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc
     5581 cagtcctccg cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat
     5641 cgtctcgatc actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg
     5701 cgcggcgttt actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac
     5761 ctgtccggca accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc
     5821 attgcatacc tgcgccggac tgccgcccag tagttcagtt aaccctgccg ccgccattga
     5881 acaggccacg ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat
     5941 tttatacagc gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt
     6001 taccggacga cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc
     6061 gttagtcggt gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc
     6121 gtacatgttg atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac
     6181 cagctgacga cgcagcgcta cggcacgacg cggcacattg agcggaccag gcagcacgcc
     6241 ttcagtgttc atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc
     6301 tgcgtcaatt tccgctttac tgcgcagcgc tagctcgttg tgcatcatca ggccagatat
     6361 agacaggccg ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg
     6421 tacggacgtt tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc
     6481 gccgacagag taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg
     6541 cattccgttc tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg
     6601 aaaatcaaca atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc
     6661 tataaatgca gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat
     6721 gataatggcg acatccgtgg catggccttt gcccgtcagt gacaacgacc catacagatc
     6781 gaccacaata tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact
     6841 ttttccggca ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa
     6901 aatatcgaat gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg
     6961 aacgaccttg cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca
     7021 atttccagct tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag
     7081 gtcgcctgat gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc
     7141 agatcggtga tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc
     7201 acgatcgctt tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc
     7261 tcaccggtct gcgggcaaac cggaatttgc ccggaggtga agaccatgct ccctaaatca
     7321 acgccctgaa cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc
     7381 atacatcctc cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg
     7441 ttcacgggtc agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg
     7501 atatttttcc gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt
     7561 gaggtgttga ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac
     7621 aaggttggtt ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt
     7681 gtacgagatc ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc
     7741 cacggcacct ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc
     7801 ggcgcgacga ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat
     7861 agtcagaatc gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt
     7921 cataaagcgt tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta
     7981 ctgcggatat tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg
     8041 tttcacgcgg gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat
     8101 gccgcatgcc atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta
     8161 gctgtacttg tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag
     8221 ccagtccatg aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat
     8281 cagcggtgct gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat
     8341 tgcgtagagc agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc
     8401 aatcaccatt gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg
     8461 catcagatcg tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata
     8521 ttttttgaag gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc
     8581 cggccccata gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg
     8641 accgtccagc cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag
     8701 cgaatcaaat tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc
     8761 gatcagttcc tgtgcctgct gctcattgag tacgccagct ttaaagtcgc gctcaatgta
     8821 gatatcgagg aacgatgccg tgcggcccag cgacatcgcg ccgccatttt gcgatttcac
     8881 tgccgccaga taagcgaagt agagccactg caccgcttcc tgcgcattct gcgccgggcg
     8941 agagatatca aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg
     9001 ctctgccagc tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg
     9061 agactggaga tcggcaaatt gcagttcgcg ttcacgtacc agataactga tgccatacag
     9121 cgctacgcgg cgatagtcac cgataatgcg cccacggcca tagccatctg gtaaaccggt
     9181 cagcacgcca gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg
     9241 gttatgggtt ttacgcagat cggtaaacag atattcaaat tcactgtcaa tttctcggcc
     9301 ataggcgtgg aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt
     9361 caacggcgca tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc
     9421 atcatgagcg gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc
     9481 gttttcgata cggatacctt ccattacttt ttcccacaat tccgtggtgg caggcgtcgc
     9541 ttcggcgagg aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg
     9601 gacattaatt tcgtttttcc agtccgtacc tttaaagcca agccatgcgt cggcgtacag
     9661 cttatcgctg gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact
     9721 aaattatgca aattctgcgg gcgcgttaac tttgcctaaa tgaatggcat ccaaagcaat
     9781 cattttttct tcgttagtcg gaataacggc acaaatgaca cgcgcatttt cactggaaac
     9841 aattcgctct ccaaaagagt tagatcgatt attcatttct gtatcaatca ctacgcctaa
     9901 tacagccaaa tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc
     9961 ggtgaatata attccatcca ggcgacgtaa tgaagctgcg tgtccggcaa tatgacgggc
    10021 aattcggtga acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc
    10081 tttttccaga acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt
    10141 attcactacg cgttccaggt cacccagact ctggttggtt tggctggcga cccaggacat
    10201 cgcaccaaag tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat
    10261 tcccattgaa gtatcaacac tctgaccgtt gcgaaccgcg cagattgacg cgccattgcc
    10321 aagatgcgcc acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg
    10381 ctgggaaaca tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc
    10441 ataatatttc cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact
    10501 ggtatcaaat accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc
    10561 acttaaattg gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat
    10621 gacttcatcg gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg
    10681 gccaattaag gccacactgt catttaaatt ccgtttttcc agttcaaatg caattgcctt
    10741 caatgcacct tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa
    10801 gaatgcattt tccgagttaa taccgtcggc aatacctgac attaatactt cacagtcgct
    10861 ggcatcgagt acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg
    10921 aaattcattc atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt
    10981 tcaggatggt cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt
    11041 tcgccagaga cggcgcttta cggatggcat acatcggcaa caggcacagc agggatgcga
    11101 taatcggtgc gcccatggct tcaataaggt cgaggatgtt cgggttggcg taggcaacaa
    11161 cccaggtgga gcccatgatg aagatcatgc tgatagtgtt cagtttaccc agcgacactt
    11221 tagttttgtc gcctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc
    11281 ccagatagtg accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat
    11341 attccagtgt aatcgcgaac gttgttttgg taccggtcat ggacgcaaag tggttagcca
    11401 gataagaaag cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagcg
    11461 taaacaggca gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag
    11521 aaatgatttg ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt
    11581 cacgtttaga aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga
    11641 tggaaatccc cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac
    11701 cgaggtcaac ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca
    11761 gcaccaggct ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct
    11821 taccaaacca gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat
    11881 tcagcggtgc aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa
    11941 cgccataaat ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc
    12001 cgccagtttt accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc
    12061 cagaaagaca cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata
    12121 ccaacatcag aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca
    12181 ccccggcgcc gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc
    12241 aggacgattg ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc
    12301 aacgaattaa ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat
    12361 tgccgccgga aataatactg acggtttttc tgttttgaat atattggtct aatttaccgc
    12421 ttaataatgc agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa
    12481 ttaaggcaat catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta
    12541 attcacgaac gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca
    12601 gggtgccggt agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt
    12661 gaacgttttc agactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca
    12721 ccgcaatacc agcaattaaa ccgccaccac caattggcac aatcacgtta tcgacatcat
    12781 agagatcttc cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat
    12841 cgtaaggtgg gataaaaata cggccttcca tttcgacaat ttcgctcact ttagcgatag
    12901 tgtcgttgaa attatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta
    12961 ctttggattt tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc
    13021 aggagagcga gaccccttgc gcatggttgc ccgcagaaca ggccactaca cctttacgtt
    13081 tttccgcatc agtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg
    13141 tacgctgcat attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt
    13201 ttgagcgagg catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa
    13261 tgtcatcaat agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga
    13321 attaaatatg taggtaaccg taaccgacac ct
//
LOCUS       DAZFRN010000132        13209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFRN010000132 DAZFRN010000000
VERSION     DAZFRN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.134"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..228)
                     /locus_tag="ACHKUB_003775"
     CDS             complement(<1..228)
                     /locus_tag="ACHKUB_003775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656736.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKTQ"
     gene            165..1664
                     /gene="pitB"
                     /locus_tag="ACHKUB_003776"
     CDS             165..1664
                     /gene="pitB"
                     /locus_tag="ACHKUB_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO6656737.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1713..2405)
                     /gene="yghT"
                     /locus_tag="ACHKUB_003777"
     CDS             complement(1713..2405)
                     /gene="yghT"
                     /locus_tag="ACHKUB_003777"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6656738.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANRPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2579..3292
                     /gene="yghS"
                     /locus_tag="ACHKUB_003778"
     CDS             2579..3292
                     /gene="yghS"
                     /locus_tag="ACHKUB_003778"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6656739.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3324..4082
                     /locus_tag="ACHKUB_003779"
     CDS             3324..4082
                     /locus_tag="ACHKUB_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656740.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4128..5477
                     /locus_tag="ACHKUB_003780"
     CDS             4128..5477
                     /locus_tag="ACHKUB_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO6656741.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5477..6301
                     /locus_tag="ACHKUB_003781"
     CDS             5477..6301
                     /locus_tag="ACHKUB_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656742.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6313..6873
                     /locus_tag="ACHKUB_003782"
     CDS             6313..6873
                     /locus_tag="ACHKUB_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6656743.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6904..7974
                     /gene="lptF"
                     /locus_tag="ACHKUB_003783"
     CDS             6904..7974
                     /gene="lptF"
                     /locus_tag="ACHKUB_003783"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6656744.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7971..9050
                     /gene="lptG"
                     /locus_tag="ACHKUB_003784"
     CDS             7971..9050
                     /gene="lptG"
                     /locus_tag="ACHKUB_003784"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6656745.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9086..10258)
                     /locus_tag="ACHKUB_003785"
     CDS             complement(9086..10258)
                     /locus_tag="ACHKUB_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6656746.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10258..10506)
                     /locus_tag="ACHKUB_003786"
     CDS             complement(10258..10506)
                     /locus_tag="ACHKUB_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6656747.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10538..11452)
                     /locus_tag="ACHKUB_003787"
     CDS             complement(10538..11452)
                     /locus_tag="ACHKUB_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6656748.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11449..13170)
                     /locus_tag="ACHKUB_003788"
     CDS             complement(11449..13170)
                     /locus_tag="ACHKUB_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO6656749.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKAL
                     PEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRC
                     VSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRSTVSVAPPF
                     GYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCY
                     GLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHG
                     IEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGY
                     LYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISD
                     EERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASL
                     HVQESLA"
BASE COUNT         3163 a         3265 c         3432 g         3349 t
ORIGIN      
        1 ctgtgttttg attatttcta aaggttttga atgaatgctt attgtctgat acacaagaaa
       61 taacactctt tttatcgtta aaaaatgata tttcactttg cccatgccgt taaagtcact
      121 gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta atatatgcta aatttatttg
      181 ttggccttga tatatacaca gggcttttgt tattgcttgc tctggcattt gtgttgttct
      241 acgaagcaat caatggtttt catgacacgg cgaatgcggt ggcaaccgtt atttatactc
      301 gtgccatgca accacaactt gctgtggtga tggcggcatt ttttaacttt tttggcgtgt
      361 tattgggcgg acttagcgtt gcctatgcca ttgtccatat gttaccaacc gatttgttgc
      421 tgaatatggg gtcaacccac ggtctggcga tggtcttttc catgctgctg gcggcgatta
      481 tatggaacct ggggacgtgg ttcttcggtt taccggcctc cagttcgcac accttgattg
      541 gcgcgattat cggcatcggt ttaaccaacg cgctgttaac cggctcatcg gtgatggatg
      601 cgttaaacct gcgtgaagtg accaaaattt tctcctcgct gattgtttcc cctatcgtcg
      661 gcctggtcat tgcgggtggt ctgattttcc tgctgcgccg tttctggagc gggacgaaaa
      721 agcgtgaccg tattcaccgc attccggaag atcgcaaaaa gaaaaaaggc aaacgtaaac
      781 cgccattctg gacgcgtatt gcgctgattg tttccgctgc gggcgtggcg ttttcgcacg
      841 gcgcgaacga cgggcagaaa gggatcggcc tggtaatgct ggtactggtg gggattgccc
      901 ctgctggctt cgtcgtcaat atgaatgcgt ccggctatga aattacccgt acccgcgatg
      961 ccgttaccaa cttcgaacac tacctgcaac agcatcctga actgccgcag aagttgattg
     1021 cgatggaacc tccattgcct gcagcatcga ctgatggcac gcaagtaaca gagtttcact
     1081 gtcatccggc aaataccttt gatgcgattg cgcgcgttaa aacgatgctg ccaggcaata
     1141 tggaaagtta cgagccgtta agcgtgagtc agcgcagcca gctgcgccgc attatgctgt
     1201 gcatctctga tacctccgcg aagctggcga aactgccagg cgtcagtaaa gaagaccaga
     1261 acctgctgaa aaaactgcgc agcgatatgt taagcaccat tgagtacgct ccggtgtgga
     1321 tcatcatggc ggtagcactg gcgctcggca ttggcaccat gattggctgg cgtcgtgtag
     1381 cgatgaccat cggtgagaag attggtaagc gcggcatgac gtatgcgcaa ggcatggcgg
     1441 cacaaatgac ggcggcagtg tctatcggcc ttgccagtta tattgggatg cctgtctcca
     1501 caacacacgt cctctcgtct gcggtagcag ggacgatggt ggtggacggc ggtgggttac
     1561 agcgtaaaac ggtaaccagc atcctgatgg cgtgggtgtt tactttaccg gcggcaattt
     1621 ttctttctgg tgggctgtac tggatagcat tgcagttgat ttaaccttcc tgaaaatgcc
     1681 cggtcctggc gatcgggcat ttccattttt gactagtgat aaccacgcgc ggtcataaaa
     1741 tccgtaatcg ctttttctgc atcaaccaac acctgttcca gtggccgatt ggcatcgata
     1801 tcaaccagtt gtgcaccacc aaaggttaac tgtggcgtta tggcaatctt cctcgccagc
     1861 gattcccgtt tatggtcggg tttacgtgca caggcaactt caaggtcaac attgagtttg
     1921 atgaccagat caggcttatg gctcgccatc cagtgaaacg ctttacgttc ctggcttgcc
     1981 agccatgaga caaaacgacc accttcaacg ttaggtggga acaccgtacc atcgtaagcg
     2041 ccaggaattt ggtcctgagg ataacggtcg gttagaacaa ttaacccgcg acgacgacag
     2101 gcaagcatat gacgaaagcg cagtaagcga cgggcgacaa acgctgtaat taccagcgcc
     2161 ggaactggtc ctggcaattt ttttgctgtt ttcacctgat ttcgttcaat tgttttatgt
     2221 aaggattttc ccatcaacgg taattttgtc actgcacgac cgacatttcc ggcctgtttt
     2281 cctaaatgaa ctctttcggc agcaccatat ttttcgacaa cggtaatgag atgttcacac
     2341 accgttgact tgcctgaacc atcgctacca ataacggcaa ttaatggagg tgttattgac
     2401 tgcatgaata tatccttaaa tttaaatatc catccaaaat atttatttgg ttaatatgtt
     2461 tttatgaaag cgtaattcag gtcaatgtca caattaacca ttgtcacaat aaggttgaac
     2521 ggatattctg agcacggacc acgtaatatt caatacatta tttactgccg ggtttttcgt
     2581 gaatcaacgt aatatgtcaa taattaattc cacgccagtg cgtgttattg ccattgtagg
     2641 atgtgatggt tcaggtaaat cgaccctcac ggcaagcctg gtaaatgaac tggcagcaag
     2701 aatgccaaca gaacgcattt atctcgggca atcgtccggg cgaattggcg aatggatttc
     2761 acagctccct gttattggcg caccttttgg gcgttatctg cgaagtaaag cggcacatgt
     2821 gcacgaaaag ccctcaacac cgcctggcaa tattactgcg ctggttatct atctgctttc
     2881 ctgctggcgg gcgtacaagt ttcgcaaaat gttgtgtaaa agccagcaag gctttctgct
     2941 catcaccgac cgctacccgc aggttgaagt gccggggttt cgctttgatg gcccgcaatt
     3001 ggcaaaaacc acgggcggta acggttggat aaaaatgtta aggcagcgcg agctgaagct
     3061 gtaccaatgg atggcgtctt atttgcccgt attgttgatt cgtcttggca ttgatgaaca
     3121 aaccgcgttt gcgcgtaaac ctgaccacca actggcagcg ttacaggaaa aaatcgccgt
     3181 tacgccgcaa ctgacattca atggcgcaaa gatccttgaa ctggatggtc ggcaaccagc
     3241 cgatgaaatt atgcaagcgt cactacgcgc aattcacgcc gcgctttcct gatcaccaaa
     3301 atggataggg gatgatttaa cgaatggatg cactacaaac acaaactgtt aatagtacaa
     3361 ccgcaccgca gcccaactac attccggggc tgattgcggt ggtcgggtgt gatggcaccg
     3421 gtaaatccac gctgaccacc gacctggtga aatcgctgca acaacactgg caaaccgagc
     3481 gccgctattt ggggctgctc tccggcgaag acggcgacaa aatcaaacga ttgccgttgg
     3541 ttggcgtctg gctggaacgg cgactggcgg ccaaatcctc gaaaacccaa agcatgaaaa
     3601 ccaaatctcc ggcgctatgg gcggcggtga ttatgtactg cttctcgctg cgaagaatgg
     3661 cgaatctgcg caaggttcag cgactggcgc aaagtggcgt tctggtggtc agcgatcgct
     3721 tcccgcaggc tgaaatttcg ggcttttatt atgacggacc ggggattggc gtcgaacgtg
     3781 cgaccgggaa aatcagcatg tttctggcgc agcgcgaacg gcgtttatac caacaaatgg
     3841 cgcaatatcg cccggaatta attattcgcc tgggcattga tattgagact gccatctccc
     3901 gcaagcctga ccatgactat gccgagctgc aggacaaaat cggcgtcatg tcgacgattg
     3961 gctataacgg cacaaaaatt cttgagattg attccagagc gccctacagc gaagtgctgg
     4021 aacaggcaca gaaggcggtt tctctggttg ccatcgtttc tgaccgccga agtttaactt
     4081 aaggctggcg ggatactttt tactatcaac gataaggtca ggctgaattg gcgggtttta
     4141 acatcaaaca ttggtttgca gatggcgcgt ttcgcaccat tattcgcaat agcgcctggt
     4201 taggctccag taatgtcgtg agcgccttgc tggggctgtt ggcgctctcg tgtgccggta
     4261 aagggatgac gcccgccatg tttggcgtac tggtgattgt gcaatcgtac gccaagtcga
     4321 tcagcgattt tattaagttt cagacatggc agctggtggt tcagtacgga acgccagcct
     4381 tgaccaacaa taatccgcag caatttcgca atgtcgtctc attttccttc tcgctggata
     4441 tcgtcagcgg cgcggtggcg attgtcggcg gcattgcctt actgccattc ctttcccatt
     4501 cattaggtct ggatgaccaa agcttttggc tggcggcgct gtattgcacg ctcattcctt
     4561 caatggcttc ctccacgccg accggcattc tgcgtgcggt agatcgcttc gatttaattg
     4621 ctgtacagca ggcgacgaaa ccttttctgc gcgcagcggg gagcgtcgta gcctggtatt
     4681 ttgactttgg ttttgcgggt tttgttattg cctggtacgt gtcgaatctg gttggcggca
     4741 ccatgtactg gtggtttgca gcgcgcgaat tacgccgcag aaatatccat aatgccttca
     4801 aattgaatct gtttgagtct gcccgacaca tcaaaggcgc gtggagtttt gtctggtcaa
     4861 ccaacattgc ccactccatc tggtcggcgc gtaactcgtg cagcacagtg ctggtgggga
     4921 tcgtgttagg acccgctgcc gccgggttat ttaaaatcgc catgacattc ttcgacgccg
     4981 ccggaacgcc agcgggtttg ctgggtaaaa gtttttaccc agaggtgatg cgtttagatc
     5041 cgcgcaccac cagaccgtgg ttgttaggtg tgaagtccgg tttactggcg ggcggaatcg
     5101 gtattctggt ggcgctcgcg gtgttgatcg tcggcaagcc gctcatttcg ctggtgtttg
     5161 gcgttaagta tctcgaagcg tatgacctga ttcaggtgat gctgggcgca attgtgattt
     5221 cgatgctggg cttccctcag gagtcattgc tattgatggc gggaaaacag cgcgcttttc
     5281 tcgtggcgca aaccatcgcc tcaatcggct acatcgtatt gctgttcatg ttctgtcatc
     5341 tgtttggtgt gctgggagca gcgtttgctt acttcggtgg gcaatgtctg gacgtggcgc
     5401 tctcgttaat tccaacctta aaggcatttt ttcagcgcca ttccttgctt tataacgcag
     5461 ccggagagaa atcctgatga tgagaaaata tttcccgctt gaggcgagcg agcgtctatt
     5521 tgttgcgatt gaagaagatg atgtcgttga tgcacaggtt agtttgccgc cgactattgc
     5581 actaagttgt accaccgaaa tcattcatga taattacgcg ttatgcctgc aattctggct
     5641 caatggcgtt aatcgtcagg agttactgcg tttagttcgc aaacaagcca aaggcgatga
     5701 gctgactgct gatgagcgca aacaatttaa atacatgcga gcgcgctaca agcacctgcg
     5761 ttttgcgcaa cggctgtatt taaagaagca tcaggccgga tttttattcg gcaaaacgac
     5821 cgtttttctg gggcgttttc aggacggctt tcgtaacggt aagaaaaata ttgtgtcgta
     5881 ttacggcaac ctgttacgga tttatttaag ctctccggtc tggtcgttgg tgaactattc
     5941 attacgccat agccagttag aaagcgtaag cagttttatt gcctatcggc aaaagcaaat
     6001 gcataccctg aaagagatta ttgccaaacc gcgactaacc ggcagggaat ttcatgatgt
     6061 gcgaaaaatc atcagccaac aggtttctta ttacgacacc ttaagatcgc ttgatcctga
     6121 aaataaagag gcgttacaga tttcccgttt cctcgccgcc attaatggcc tgatgggaga
     6181 taaacacgac gacatggtgg cggatgatat ggaaaaccgc cagtcgtatg atgcaccgtt
     6241 ggcgctggat agcgatattc gtcagcgctt agagctgctg atttcgcgtt ttccgctata
     6301 gccggagcaa tgatgtcatc tcgtttgatt attgcgttga ttatcatgct cttagccccg
     6361 ggtgtgcagg cacacaattt cgtcactggc aaaacggtta cccctgttta tatccaggaa
     6421 ggcggcgagc tgcttctgaa tagcgacgac gaaattcact accaaaagtg gaacagcaca
     6481 caattagcag gaaaagtccg cattattcag tatatcgccg ggcgtaagtc ggcgaagaaa
     6541 aagaactcac tgttaattaa agcggttgaa gcggcaaact ttccgcagga tcgttttcag
     6601 cccaccacca ttgtgaacac cgatgatgcc atctttggta ctggctattt tgttgttggc
     6661 aaaatcgaaa aaaataaacg ccgctatccg tgggcgcaat tcattattga cggcaatgga
     6721 cagggacgcg ttgcctggcg cttgccggag cagagttcaa ccattctggt gctgaataaa
     6781 gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc cggaagaggt agatcatgtc
     6841 attgcgctgg cgcagaaact gatcaatgaa taaaaaattc aaaggattga gggcgttagt
     6901 gagatgaagc tggttgaaca ctacatcatg cgtggtacgc gccgtctggt gctgattatc
     6961 gtcggttttc tgatctttat tttcgccagc tactccgcac agcgttatct gaccgaagcg
     7021 gcaaatggga cattagctct tgatgttgtg ctggatatcg ttttctacaa agtgctgatt
     7081 gcactggaga tgttgttacc tgttggtctg tatgtgtcag ttggcgtgac gctagggcag
     7141 atgtataccg actcggaaat taccgctatc tctgcggcgg ggggcagtcc gggacgcttg
     7201 tacaaagccg ttctttatct ggcgataccg ctaagtattt ttgtcaccct tctgtcgatg
     7261 tatggtcgtc cgtgggctta tacgcagatt tatcaactgg agcagcagtc gcagtcggag
     7321 ctggatgttc gccagttgcg ggcaaagaaa tttaacacta acgataacgg acgaatgatc
     7381 ctttcgcaga cggttgatca ggataataat cgcctgactg acgcgctgat ttatacttct
     7441 actgccaatc gaacccgcat tttccgcgcc cgttcggttg atgtggttga cccatcaccc
     7501 gagaaaccga ccgttatgtt gcacaacggc actgcctatc ttctcgatca tcaggggcgt
     7561 gacgacaacg aacagatcta ccgtaatctg caattacatc tgaatccgct ggatcaaagc
     7621 cctaacgtca aacgcaaagc aaaatcggtc acggagctgg cgcgctccgc ctttcctgcc
     7681 gatcatgccg aactgcaatg gcgacaaagc cgtggcctga ccgcattgtt gatggcgctg
     7741 ctggccattt cattaagtcg ggtaaaaccg cggcaagggc gattttcaac gttattgcca
     7801 ctgacgttgc tgtttattgc cattttttat ggcggcgacg tctgccgtac gctggtggct
     7861 aacggcgcga ttcctctcat tcctggtttg tggttagtgc ccggactcat gctaatgggc
     7921 ctgctgatgc tggtcgcacg cgacttctct ttgctgcaga aattttcccg atgaatgttt
     7981 tcagtcgcta tttaatccgt catctctttc tcggttttgc cgccgccgca gggctattgc
     8041 tgccgctttt taccaccttc aacctgatta acgaactgga tgatgtcagc ccgggcggtt
     8101 atcgctggac tcaggcggtg ctggtggtgc taatgacctt accgcgcaca ctggtcgaac
     8161 tttcgccgtt tatcgcctta ttgggaggga ttgttggcct ggggcagtta tcgaaaaaca
     8221 gtgagcttac cgccattcgc agcacggggt tttctatctt ccgtattgca ctggtggcgc
     8281 tggttgccgg gatattgtgg actgtttcgt taggcgcgat agatgagtgg gtggcgtcgc
     8341 cattgcagca acaggcgctg caaatcaaat cgaccgccac cgcgttgggg gaggacgatg
     8401 acattaccgg caatatgctg tgggccaggc gcggcaatga atttgtgacg gtgaaatcgc
     8461 tgaatgaaca gggccagcct gtgggcgtgg agatttttca ttatcgtgac gatctttcgc
     8521 tcgaatccta catttatgca cgcagtgcca ccattaaaga cgacaaaacg tggatcctgc
     8581 atggtgtgaa tcataaaaaa tggcttaacg gtaaagaaac gctggaaaca tcagataatc
     8641 ttgcctggca atcggccttc accagtatgg atcttgatga gttatcgatg ccggggaata
     8701 ctttttctgt ccgtcagctt aatcattaca tccattattt gcaggaaacc gggcaaccca
     8761 gcagcgaata ccgccttgca ctgtgggaaa aactggggca accgatcctg accctggcga
     8821 tgattttgct ggctgtgccg tttaccttta gcgccccgcg ctcgccaggg atgggtagcc
     8881 gtctcgctgt aggcgtcatc gttggcttac tcacctggat cagctatcaa atcatggtca
     8941 atttgggatt gctatttgcg ttaagcgcac ctgttaccgc gctcggttta ccggtagcgt
     9001 ttgtgttggt ggcgttgagc ctggtgtatt ggtatgacag acaacattaa acccgccgtg
     9061 gcgcaaacac cacggcgggc tcacattact gcttcagagt agcaaatgcc tgaatgatgg
     9121 catcgatttg ctcacgactg tgggcagcat ttacgctaca gcgcagcagc gtgatgcctg
     9181 caggcgcggc aggcggcagg ataagattca catacacgcc cagcgagata agctcgcgcc
     9241 agatgcgtaa accttcctct ttcgaaccga ttattactgg cactaccggg ctaatatgtg
     9301 aacttaactc ataacccagt ttttgcagac cgtcatacag atggttggcg ttatccatca
     9361 gcttttgccg cagctctggt tgtgtggcaa tggttcttaa cgatgagcgc acggtcgcaa
     9421 tgcaggacgg agaaggagag gcggtaaaaa tatacgggcg gctgccgtag cgcagcactt
     9481 ccatcgcttc cgaaccgaca gcaaaaccgc caatcgaggc cagactttta ctgaaagtac
     9541 caacgataat atccacatca tcttcaacgc cgacagcttc agccagtccg cgccccgttg
     9601 cgcctaacac gccaaacgaa tgcgcttcat cgacaatcaa ataaccaccg agacgacgtt
     9661 tgatatcaac aatttccgcc agcggcgcaa cgtcaccgag catgctgtag atgccctcaa
     9721 cgatgataat cgcctctttc gcccgttcac cgaggcgaac catgcgccgt tccagatctt
     9781 tagcatcgtt atggcggaaa cgaataattt ccgcgccacc gagagaacag gcgtcataaa
     9841 tactggcgtg gctgtcggca tccagtaaca ccacggcgtt atggtcggca agtgcgctga
     9901 tgacgcctaa attagcggta taaccggttg aaaaaacgat agcggtaggg cgattgaaaa
     9961 actcggcaat ctctttctct aacgccaggt gcggcgcata actgccgttt gccatacgag
    10021 aaccggtggt gccggtacct tgggccgcta acgcggcctg accttcgctg atggcatcat
    10081 gattaaaggt taaaccgaga taattattgg tcccggccag aatcactttt ttattgccga
    10141 tccggccttc ggtggcggaa tacacttcat caatacaggt gccaaacggt gttaacccgt
    10201 tgtcactaaa ttgcagtcgc tcgccagcaa ggcgcgcata tttatcgtat agccccatta
    10261 tttattctcc agccatggaa gtagagtttc cattaattgc gcaggggttt tgacatccag
    10321 cagaatatta ataggaatgg aaatatcgaa tctgtcttcc agcatcatta agagatccat
    10381 cactttgatc gactccaggc caagatcgtt gacgagatcg ctgtcgggct taatttccac
    10441 accgttttcg accaaatcct gtaaacagga aaggatgtaa tccattacta tttcacgatt
    10501 taccatagaa acgttaacac acctttaaac aagacaatca aaatagcccg ttgcgtaatg
    10561 cgtgttccag gctaatcccg ggaaaccaat taatatcttc agaaatacgg ttattacttg
    10621 ccgaccagtc ggcgtgggtt aattcacgaa ttttcgagcg ggtcagcata ggttctttac
    10681 ctgccaggcg actcaacgcg gtactgatat ccgcaaggca ggtgagtacc ggcagaggaa
    10741 taccaaccat tcgcacggaa ccgcaacgga cgttggcggc aagttgctgt atgcgttgcc
    10801 agtcatagcc gccagcgacg ccatcgcata attcataggt ttgcgtctgt acagtttcgg
    10861 cgcttaacca ctgacccact gcttgcgcaa aatcggtgac gtgcaggaaa gagagctgtg
    10921 tatctggtgc accaagtcgt ggcagcaggc cgcgcagcat ccagtcaaac agcggtttta
    10981 actctttatc gccgggacca tacacggctg tcgggcgaaa aacgcccagc gtaatttcgt
    11041 cagccattgc agtcagccgt tgttcggcga tgtgtttgga atttgcgtac caggagagct
    11101 cgggatggcg cgccgccagc gaagagataa acagaaaacg ttggcaaaag cagctctctt
    11161 ttgctgcttg catcaggcgc aggctgccgt caacgttaca gcgggtgaaa atctcttctt
    11221 tgtgcccgcg cacttgcccg gcgcaatgga ccactgcgct ggctccggca accagcttgc
    11281 taagtgaatg agtatcttcc agcgaaccgc gcacccaggt gagattatca ttgacgtgag
    11341 cgcgggcagt acgcgtcaat gcgcgaacgt gaaagccgcg ggcgagcagg ttatcgataa
    11401 tatatttacc gataaacccg gtagcgcccg tcaccgcgac agtttgattc atgccaggga
    11461 ttcctgcaca tgaaggcttg cagcataagc cttctgataa cgttttttcg cttccgcacg
    11521 ggcaggcttg ccggaggatg ttcggggaat actgtggggc ggcaacagat cgatagccgc
    11581 tgttacgcca aattcgcttt ggatccgtgc tgccagcgcg tggataagct gcccgcgacg
    11641 ttcttcgtcg ctgatccgac actggatctg caaaatgatt ttttcctggg cggtaacaaa
    11701 agcaatcgca tcgccagaat gaatttccgg ttcttgttcc gcaatatatt caatatcctg
    11761 cggccagata ttacggccac gaataataat cagatcttta atacgtccgg tgacatacag
    11821 ataaccgtcc agcagataac cgaggtcgcc ggtgtctaac cagcccgtcg cggcaatctc
    11881 gtcttgcgaa acctggtcgc caaagtaacc gctcatcagg ctggggccgg agatgcaaat
    11941 atggcctacc acacgttccg cgaccggcat acctgcttca ttgcggattt caatgccatg
    12001 ttccggcaac gctttgccgc agttgacgaa agtcgatacg gcgcgtgttt cagcacccgg
    12061 cgcgacggct ttgccctgat attcgaggat gtcgcgatcc acttcgttaa ccacaacccc
    12121 agagccttca tcagagaagc tgacagccag cgcattttct gccagtccgt agcacggcat
    12181 gaacgtttta tcgtcaaagt taacctgacg gaaacattca gcgaattgat ggagttgttc
    12241 tgcggagatc ggctcagcac caataccagc gacgcgccag caggaaagat ccagttcagc
    12301 gagatctttt tcattcacgc ggcgctggca caattcatag ccaaacggcg gcgcaacgga
    12361 aacggtgctg cgatttttac tgatcaattt aagccattgc agaggacgca tggcaaaatc
    12421 ctgagtgcgc aaataatcta ctgaaagttg cgtggcgacg ggggtcagga gaaagccgac
    12481 cagtcccata tcatggtaga aaggcagcca ggagacgcag cggtcgccag ggcgtaattt
    12541 aatgccatcg tggcttatag cacgtagatt agccatcact tcgcgatggg tgataatgac
    12601 gccacgggga aaacgggtgc tgccggaggt gtactggagg taggcgatat cgttcggaac
    12661 tggacgctgg agcgcaacat cggcttccgg taatgcctta aaccaggcgt ggcttaaaac
    12721 atgtaattcg gggttgttat gcgtcgcggc attgaccagt ggcaaccact catcaccagt
    12781 gataatggct gcgggctggc aacttgccag taaaccctgg agtttggcgc tccaggaatc
    12841 ccgctgaccg acgcccattg gaatcgccaa cgggacggcg actaagccag catactgaca
    12901 ggcaaaaaaa gcctctacaa acccgctact cgtttcggca atcagcgcca cgcgttcgcc
    12961 ttttttcagg ttcagcgata acaaccgctt cgcaccagct tcggcacgcg tttttaatgt
    13021 ctgatattcc agttgatctt caagttggca acgtctgtca taaaaattca ttccggcgct
    13081 actcagagcg gcgtagtcca aagcatcaac cagcgttgga aaatcggcat agcgcatagg
    13141 tagggaatgc gtaaagattt tattagacat atacaccacg aaaaaattgg atatcaaaaa
    13201 taaaatgac
//
LOCUS       DAZFRN010000133        13096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFRN010000133 DAZFRN010000000
VERSION     DAZFRN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.135"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(112..324)
                     /gene="cspH"
                     /locus_tag="ACHKUB_003789"
     CDS             complement(112..324)
                     /gene="cspH"
                     /locus_tag="ACHKUB_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO6656750.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            610..822
                     /gene="cspG"
                     /locus_tag="ACHKUB_003790"
     CDS             610..822
                     /gene="cspG"
                     /locus_tag="ACHKUB_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO6656751.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            833..1021
                     /gene="ymcF"
                     /locus_tag="ACHKUB_003791"
     CDS             833..1021
                     /gene="ymcF"
                     /locus_tag="ACHKUB_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO6656752.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            996..1226
                     /gene="ymcE"
                     /locus_tag="ACHKUB_003792"
     CDS             996..1226
                     /gene="ymcE"
                     /locus_tag="ACHKUB_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO6656753.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            1216..1389
                     /gene="gnsA"
                     /locus_tag="ACHKUB_003793"
     CDS             1216..1389
                     /gene="gnsA"
                     /locus_tag="ACHKUB_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO6656754.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1438..2511)
                     /gene="yccM"
                     /locus_tag="ACHKUB_003794"
     CDS             complement(1438..2511)
                     /gene="yccM"
                     /locus_tag="ACHKUB_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6656755.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(2583..5327)
                     /gene="torS"
                     /locus_tag="ACHKUB_003795"
     CDS             complement(2583..5327)
                     /gene="torS"
                     /locus_tag="ACHKUB_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO6656756.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPTLNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPSALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAMRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAASLVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5410..6438
                     /gene="torT"
                     /locus_tag="ACHKUB_003796"
     CDS             5410..6438
                     /gene="torT"
                     /locus_tag="ACHKUB_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO6656757.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6411..7103)
                     /gene="torR"
                     /locus_tag="ACHKUB_003797"
     CDS             complement(6411..7103)
                     /gene="torR"
                     /locus_tag="ACHKUB_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO6656758.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7233..8405
                     /gene="torC"
                     /locus_tag="ACHKUB_003798"
     CDS             7233..8405
                     /gene="torC"
                     /locus_tag="ACHKUB_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO6656759.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8405..10951
                     /gene="torA"
                     /locus_tag="ACHKUB_003799"
     CDS             8405..10951
                     /gene="torA"
                     /locus_tag="ACHKUB_003799"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO6656760.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10948..11547
                     /gene="torD"
                     /locus_tag="ACHKUB_003800"
     CDS             10948..11547
                     /gene="torD"
                     /locus_tag="ACHKUB_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO6656761.1"
                     /translation="MTTLTAQQIACIYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNALENRIAALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVARCHQHDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(11640..11945)
                     /gene="cbpM"
                     /locus_tag="ACHKUB_003801"
     CDS             complement(11640..11945)
                     /gene="cbpM"
                     /locus_tag="ACHKUB_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO6656762.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(11945..12865)
                     /gene="cbpA"
                     /locus_tag="ACHKUB_003802"
     CDS             complement(11945..12865)
                     /gene="cbpA"
                     /locus_tag="ACHKUB_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO6656763.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3183 a         3324 c         3429 g         3160 t
ORIGIN      
        1 cgcttaaatg gattagtttc tttgtggaaa agtaaccgat gttataatgt atgatgttga
       61 ctattgcact attccaataa aaccagtcag ctttaaacaa gcagcgtcat attaagagag
      121 ataaacattt gccgctgttg gtcctcgcag gccatttacg cggcaaaatt ccacacgtaa
      181 tcctggtata agcacttctg cgtcgcgggg agtgaatgcg gaaatatgga cctgaacttc
      241 tttacgaccg tcggagggga taatgaatcc tttgccgctt ttgcgatcaa aggttttgac
      301 aattcctgtc attttacggg acaaacaaat tccttactga aaatactgcg ctgcactata
      361 cggggttaat aaaataaagc cagcgatatt taagaccgcc ggacggctaa aataaaattt
      421 gcttaatctc aattatcatg ccttaatagc tgcgtcggtt tgaaagacag acagcataca
      481 aagtagttta ctaaagcagt tctcattatc aggcattatc cccttctttt gagtctctct
      541 cctgaacact aagtagtttc tgtattaaag ccctgtttgc cgaaaggccc aaaatgaagg
      601 aagtaaaata tgtctaataa aatgactggt ttagtaaaat ggtttaacgc agataaaggt
      661 tttggcttta tcactcctga tgatggcagc aaagacgttt tcgtccattt caccgccatc
      721 cagagcaatg aattccgcac gctgaacgaa aatcagaaag ttgaattttc tattgagcag
      781 gggcaacgtg gccccgcggc agcgaacgtt gttacgctct aaggttgccg ttattactca
      841 acatctccat ttccgctgtc catgttgtca tggttcacag taccgtacat cggcattcga
      901 tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt tgtaaatcga cgatgatcac
      961 ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg ccgctggatt tcacaaaata
     1021 atatcaggct ccctcgtgga gcctttttta tatctgcctt atttttcttc aacgctgtat
     1081 gtatagtaag cgataacctg ttgattattg aatctttcgg ggagatggct tataacattt
     1141 cttacctgac cagggtaccg ggaaccaaca ccttactggc gtgttgctgt ctttcaagac
     1201 cagacgaggt taacagtgaa tattgaagag ttaaaaaaac aagccgaaac ggaaatcgct
     1261 gactttatcg cgcaaaaaat cgccgagctg aacaagaata cagggaaaga agtctctgaa
     1321 attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa
     1381 ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta
     1441 gtgaccaatc attggcgctt gtggaattaa gcgtcggtac aattcctccg gcaccgggct
     1501 ttgccatact cccgcataca ttgcgtaacc aatcaccgcg aacataatcc ccataaccag
     1561 tagcgtcatt aaccagccag acaacgcaaa agctttttta tttgccgcag gtttttgcag
     1621 tgaaaaggtc aatgttgagg ctaccggaca tgactctacg caagtcatac agccggtaca
     1681 ttccactgtt cgtacctgaa ttaatttatc taccgggatc cgtgatgggc aattttttgc
     1741 gcatttgcca cagtcgatac aactttcggc attgcgacga atcttaaacg gcgacaatag
     1801 cgaaaccacg cccatcagcg cgccatatgg gcaaagataa cgacaccagg catggcgaat
     1861 aaacaggctg gcaatcagca aaacggtcac gctgattaat gtcgcggtcc ccatatgacg
     1921 aaagaaatcg agcattttta cgtccatcac cacgctgtag ggcgacaaca taaaatagtg
     1981 aatcgcctga gcgggcatca ataacgcgat atagagaaaa aaactcaaca gcaaatactt
     2041 cacgccgcgc agaggaatat ccagccagcg gggaaggaca cattgccgac caaacagttt
     2101 gttaccgaga tcgccgatta attcagaaag cgtaccaacc gggcataacc atgagcaaaa
     2161 ggcctttttg agtaatagac tgatgacgat aaaagcgacc aataacagca tcgcggcggc
     2221 gtggacggac ggtaactgac ctgttgtaag gctatatttc agattcatca gaccggcaat
     2281 cggtagccag ccttcgatac ctcccggtct ggcgacaaat gtcgtgctac ttgccgtttc
     2341 gtaatagcgc acccaatacc agaacgtgat ggcaatataa atattaattg ccaacagtaa
     2401 taattgcgtc gctttacgcc aggtcgtggc attgcgccag tcattccacg gtaatttgcc
     2461 gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt ttctctgcca tgattttgcc
     2521 agtccgttaa gttgcataca aaatgccact attctagttg ttcttaacag gctgatattg
     2581 attcaaatcg cgttcaggtc tttcttatgc aaccatgctt ccagagcggc aacactgcgt
     2641 gtaatttctt cgtgtggaag gggggcagat aatggctgct gctccagttg tgcgcatagc
     2701 tggctggcga tacgcattcc caaactcgag caactgcttt ttagctgatg cgcggcacgc
     2761 tttattttct cgctatcctg actggcgcgg gcaatgtcga tttcatcgag aagcggcagg
     2821 gcatgttgtg taaataatac cagccattcg tggatcttct ccgtccccat taactgagca
     2881 tcttcattga gttgcgatac atccagcgat tgatcgttat tgacttgcag ttggagatag
     2941 tgcgccagta actgaccgag cacttcacgc ggcaccggtt tagggataat cccgcggaat
     3001 aatgaactgg tacgctggcg cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa
     3061 accagcgacg gatattgctg tgccagttgt cgggcaagcg taatgccgtc gacatccggc
     3121 agatcaaaat ccaccagtga ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc
     3181 tgcgcggcat tgccaacagc aacaacctgc gcaccactgg tgttcagcat ctcaacggta
     3241 attcgctggg ttagcgggtt atcttcaatt aacagcaaac gtaaaccgtc aagacgcatc
     3301 gcctgattga ctgtttttgg cacgggtgcc gtggcaacac gtaacggcaa gcgtaaacaa
     3361 aaacagcttc caacctccgg cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc
     3421 agacggctac tgatagtcag tcccagcccg gtgccgccgc gtttgccgct tacctgcaca
     3481 aatggctgga agatttctgc cagtttcgcg ggatcaatac cgcagccgct gtcttccact
     3541 tcgaccagcc attgctcgcc atcagtgcga ctacgcagga taatgtaccc ttcgtcagta
     3601 aaacgcaggg cgttgctcaa caggttggtt ataacctgac gaatacgtcg cggatcgccc
     3661 attaacgcgc tcggtacatc atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc
     3721 acccgtccgc tcattaattg cagggtactt tccagcagcg ggcgcggttc aaagggctca
     3781 tcgctgaccg aaacattctt gccacctgct tcgatagcgg aataatcgag aatatcgttg
     3841 aggatggtca gcaacgattc accagagtca gtaattgccc gcaaatcatc acgctgggcg
     3901 ttaagtgtgg ggttatctgc cagtagctgt gcggtgccga gaataccgta cagcggtgtg
     3961 cggatctcat ggctcatcgc cgccagaaac gccgattttg cctggctggc tttttctgct
     4021 tccgcccgtg cctgtcggtg ttctatcacc agttcctgca attcagctgt acgcgctttg
     4081 acctgcgccg ccagctgttc gcggtggcga ttcagtgcat gaacattgct gcgaaacgca
     4141 tccatcagcc gcccgatggt atccagctcc cgtacgccag cggtttccgg gaaaggggag
     4201 tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg
     4261 actgagcgat aaaccacgcg ccaaaggatc agaatcagtg cgcaaagtga aaccatcccc
     4321 agcaataaca ggctgtattg cccgcgtgca ctggcttttt ccagatgcgc cagtccgtgc
     4381 tgattacgca gctcaatggt gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac
     4441 tgggcgatgt tattttgtgc gagagtttgt aggtgattgc tgatttcact gtcctgctga
     4501 tacagcgcca gcaaatcgct atattggcta acggtagtta acgttgttgc gacctgcgca
     4561 cgaacacccg gatcttcaat gcgtatttgc cgacgttgca gaattttcac cgcattattg
     4621 agctgctttt ccagcgttgg tgcatttttc tggatctgct ccagccccag attcatcacc
     4681 atttgctgca cccgcagagc gctaaggcgc agttcattca tctggttaac atactcaaga
     4741 tcgatatcaa tcagccgatc gagtgcactt tcagcagcct gacgctgatc ttgttcgatc
     4801 aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg tcgcattatt cgcctgacct
     4861 tgcgccaggc gtgcgatctc atcggcggca gcgactatct gctgactgag ttgccgttgt
     4921 tgctggcgta gttgcagacg ccgccccacc agttcccctt gctgacgtaa tgaacgggag
     4981 atctcctgct cctgttgttc aatagcggtg gtatcaaaac cttgttcccg taacgcttgc
     5041 agcaacgcat taatcttcag gctttgtgcg gtgagcattc gcccctgcgc ctgccacatc
     5101 ttttcgttat cggcactggt caggttctgc gcggcgaaaa gttcccaggc gctggcttcg
     5161 ctcaactggc gcgccatatt catggtagga atcaatgcct gagtgttgtc tttttccacc
     5221 tggctgataa agcgcaggtt gtaccatccc accagggtac tggtcagggt taacagcgcc
     5281 atcagggcaa agcccatcca gagtcttcgg gtcagggtta aattcacggt cggtgcactt
     5341 taggtgaaaa aggttgagtc gcaaagcaga atgcatctag cataaagcct tattattgat
     5401 gaggctatca tgcgcgtact gctattttta cttctttccc ttttcatgtt atcggcattt
     5461 tcggctgata acctgttgcg ctggcatgat gcgcagcatt acacggtgca agcctctacg
     5521 ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt atcccagcct gaaagattca
     5581 tactggttat cgttgaacta tggtatgcag gaggcagctc gccgctatgg tgtggattta
     5641 aaagtgctgg aggcaggcgg ctacagccag ttggctaccc agcaagcaca aatcgaccag
     5701 tgtaaacagt ggggcgcaga ggccatttta ctcggtagta gcacgacctc atttcccgat
     5761 ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg taaatgctat tgatgctccc
     5821 caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg gctatcaacc tgggcgatat
     5881 ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt tgatgcccgg acccgataat
     5941 gccgggggca gtaaagagat ggtcgagggt tttcgcgcag ccattgccgg aagcccggtg
     6001 cgtattgttg atattgcgct tggtgataac gatattgaaa tccagcgtaa cctgttgcag
     6061 gagatgctgg aacgccaccc agaaatcgac gtcgttgccg gaacggccat tgcggcagag
     6121 gcggcaatgg gtgaagggcg caacctgaaa acgccgctta ccgtggtgtc gttttatctt
     6181 tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga ttatggctgc cagcgatcaa
     6241 atggtctggc agggggaact ggcagttgag caggccatca ggcaattaca ggggcaatcg
     6301 gtgtctgata atgtcagccc accgatttta gttctgacgc cgaaaaatgc cgaccgagaa
     6361 catatccgcc gctcgctgtc accgggggga tttcgtccgg tctattttta tcagcacaca
     6421 tcagcggcta agaaataacc ttcaccatgt tgcgtcacca gtaaatccgc gctgagttta
     6481 tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt cagggttttc cacccgacgc
     6541 gcagaaagca tacgtagcag acgttcacgg ctgagaattt cgcccggatt cgtcacaaat
     6601 gccaccaaca tttcatactc tgcgcgggtc agtttaatcg gctcgccatc ccgctccagc
     6661 gtatggcgcg acacattcag gcaataaccg gcaaagcgat agcagttgtc ctgagtgtgc
     6721 ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat ttttcacccg tactaccagt
     6781 tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc ccatttccag cccaacaata
     6841 cggtcaatcc gatcgctgcg tccggtaacc agaataatcc ccaccgttga gcgttctcgc
     6901 agggcgcggg ttaacatcag gccattttca tcgggtaagt tgatatccag cagaattaaa
     6961 tctaccggct gattctgcat aatttcccgc agcccggcac cgctcgctgt aacggaaacg
     7021 gtatacccct cctgagtgaa gtaagattgt aatcgcgcct gggtaaccgg ctcatcttca
     7081 acaataacaa tgtgatgtgg catcagaggg ttttactcat tctgttcata tctgttcata
     7141 ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc attaagatcg
     7201 cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac gcgctacgcc
     7261 gacccagtgc tcgttggtcg gtactggcgc tggtcgcaat tgggattgtg attggcattg
     7321 cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa ttttgtgtca
     7381 gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat ttccagaacg
     7441 cctccggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt ccaggcatgg
     7501 tgaagcgcaa actggaagcg agcaatgata tctaccagac ctttattgct cactccattg
     7561 atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa tgggcgcgaa
     7621 tgaaagaaaa caactcggca acctgccgct cctgccataa ctacgatgcg atggatcatg
     7681 cgaagcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat aatcaatcct
     7741 gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc ggcttccgta
     7801 agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg tactctattg
     7861 atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct ctgctgcctg
     7921 cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa attaccggct
     7981 ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa cgcatctttg
     8041 ttgcctcgat tcgtggtgat gtgcagcagc aggtaaaaac gctggagaaa accaccgttg
     8101 ccgacaccaa taccgagtgg agcaagttgc aggccactgc gtggatgaag aaaggcgaca
     8161 tggtgaacga tatcaaaccg atctgggctt atgcggattc gttgtacaac ggcacctgta
     8221 accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg atcggcacgc
     8281 tcaacggcat gattggcttt accagtctcg ataaacgtga agaacgcacc ttgttgaaat
     8341 atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag aaggaagaaa
     8401 aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg cacaactcgg
     8461 cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc gtgcgactgc
     8521 ggcgcaagcg gcgactgacg ctgtcatctc gaaagagggc attcttaccg ggtcgcactg
     8581 gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact
     8641 ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca acgcggcgcg
     8701 tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca gcgatacctc
     8761 ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg acatgttcta
     8821 tgaagaactg gaacgcgtgc agaaaactca cgggccgagt gccttgctga ccgccagtgg
     8881 ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag ctattgcctt
     8941 gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc aggtgatcct
     9001 gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc cgctggtatt
     9061 gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc agcaagcgaa
     9121 ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag cgaaagtcgc
     9181 cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc atgagtatct
     9241 ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc cgctgcaact
     9301 ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc tcgctaacta
     9361 ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg gtcagccgaa
     9421 agatgccgca tgggctgaaa aactgactgg cattgatgcc gaaaccattc gtgggctggc
     9481 gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc agcgtatgca
     9541 gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg ggcaaattgg
     9601 cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca cgccggggcg
     9661 taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa
     9721 cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg atgcgatcct
     9781 cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc cgctgaaaat
     9841 gtgtattttt gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga
     9901 aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct caacctgccg
     9961 ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta
    10021 cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgcagttcga
    10081 ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc gcgaagaagc
    10141 ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca
    10201 gcaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga
    10261 gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc
    10321 ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc
    10381 cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc
    10441 ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt
    10501 tcgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata cggtagcggg
    10561 taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga
    10621 tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg tttctgaccg
    10681 ctatgcaccc ggtgtggcac gaattcacga aggggcatgg tacgatccag ataaaggcgg
    10741 cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg acatcggtac
    10801 atcgcagctg gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa
    10861 cggaacagtg gagcaggtga cggcgtttaa cggccccgtg gagatggtgg cgcagtgcga
    10921 atatgttccc gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt
    10981 atttacgcct ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa
    11041 atcgccagtg cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctggct
    11101 gcggcggtga atgcgctgga aaaccgtatt gccgctctga cagtacgtga cgatgcccgt
    11161 ctggaactgg ccgctgactt ttgcggcctg tttctgatga ccgacaaaca agcggcgctg
    11221 ccgtatgcat cggcctacaa acaggacgag caagagatta aacgcttgtt agttgaggca
    11281 gggatggaaa ccagcggcaa tttcaacgaa ccggcagatc atctggcgat ctatctcgag
    11341 ttgttgagtc atctgcattt ttcgctggga gaggggaccg ttcctgcgcg aagaatcgac
    11401 agtttgcggc aaaaaacact gacggcgctg tggcaatggt taccagagtt tgttgcgcgt
    11461 tgccatcagc atgacagctt tggtttttac gcggcactaa gccagttatt gctggtgtta
    11521 gtggagtgcg accaccaaaa cagataacgt cgtttgtgcg cctgaaaaga cgcgtttagc
    11581 gtcgcatcag gcattatggg gcagttgccg gatgcgtcat cacatccggc aaacacacat
    11641 cacggatgag ttacaaaccg ggaaagtcgc tggcgcagca ggcggttttc ctgcttcagg
    11701 tgcgcaatat catccattaa cgtcagcgcc accgcgatcc caggccagtc cagagccagt
    11761 tcatgacgca ggcgtactgc gcgttgcacc acgatggcgg catggtcgtc aaatacccag
    11821 gttgtttcct ggatctcacg cggttcaacc acccccaaac cgacaatttc attcaactcc
    11881 tcttcagaga tgccggtatg caggcaaaat tcggtaatag taaaagtcac cgtaacatta
    11941 gccattatgc tttcccccaa tctttacgtg gatcaaaaga cgactgggcg tctgccagtt
    12001 gctgccacag cgcggcagtg ttttcatccg gtttcggcgg catcacgatt ttcagtaccg
    12061 catacagatc gccggtctgt tttttgctca ccagaccttt gcctttaacg cgcaatcgtt
    12121 gcccggcctg gctgcctggc gggatagtca gcaaaatgct ttctttcagt gttggaacgg
    12181 tgactttagc acccagcgcc gcttcccacg ggctaaccgg cactacaatt tccagatcat
    12241 ggccgacaat atcgaacagc ggatgtggcg caatatgaat caccagccac aaatcgccat
    12301 ttggaccgcc gttttcgccc ggcgtcccct ggcctttcag acggatgcgt tgaccattgc
    12361 cgacgcccgc cgggatcttg acattcagcg ttttcggaat ttcctgttcg atcatgccaa
    12421 aggcgttata aaccggcagg ttatagctga tggtacgctt atgctcagta agcgtttctt
    12481 cgaggaatac cgccacttcg atttcaatat cgtggccgcg tgtggcgggg cgttgacggc
    12541 tctggcgggc atgctgaccg aaaattgacg agaagatatc gtcaaaatct tcggcgttaa
    12601 aactctgacc gtcgctatga tggaactgac ggttaaattg cggatcgttg cgatgttgcc
    12661 acatctgatc atactcagcg cgacgttgtt catcacttaa cacttcccag gcttcagcga
    12721 cctctttgaa gcgggcttcg gcatccggtt ctttgctgac atcaggatgg tatttgcggg
    12781 ccagtcgacg ataggcggtc ttgattgtct tgagatcgtc cgtcggtttc acgcccatga
    12841 tggcgtaata atcctttaat tccatagcgt tatctcgcgt aaatcaacac aaattgaagg
    12901 aacccctgta aggtaactcc tataagtgta gggtaatcct caaaatttca tatgccaaca
    12961 cagaatatgt tattgaaatc atcgcggaga ggaggtcgcc tcaaggtggg gtgctgaaca
    13021 tattttaaac aggtgaaaaa gggtgagcga tttttgatag tggaaccagg cactttaagt
    13081 ttaactgggg cgtcat
//
LOCUS       DAZFRN010000134        13012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFRN010000134 DAZFRN010000000
VERSION     DAZFRN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.136"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            292..627
                     /gene="yjdM"
                     /locus_tag="ACHKUB_003803"
     CDS             292..627
                     /gene="yjdM"
                     /locus_tag="ACHKUB_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO6656764.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            787..1230
                     /gene="yjdN"
                     /locus_tag="ACHKUB_003804"
     CDS             787..1230
                     /gene="yjdN"
                     /locus_tag="ACHKUB_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO6656765.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1363..2151
                     /gene="phnC"
                     /locus_tag="ACHKUB_003805"
     CDS             1363..2151
                     /gene="phnC"
                     /locus_tag="ACHKUB_003805"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO6656766.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2176..3192
                     /gene="phnD"
                     /locus_tag="ACHKUB_003806"
     CDS             2176..3192
                     /gene="phnD"
                     /locus_tag="ACHKUB_003806"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6656767.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3254..4033
                     /gene="phnE"
                     /locus_tag="ACHKUB_003807"
     CDS             3254..4033
                     /gene="phnE"
                     /locus_tag="ACHKUB_003807"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO6656768.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4054..4779
                     /gene="phnF"
                     /locus_tag="ACHKUB_003808"
     CDS             4054..4779
                     /gene="phnF"
                     /locus_tag="ACHKUB_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO6656769.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4780..5232
                     /gene="phnG"
                     /locus_tag="ACHKUB_003809"
     CDS             4780..5232
                     /gene="phnG"
                     /locus_tag="ACHKUB_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO6656770.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5229..5813
                     /gene="phnH"
                     /locus_tag="ACHKUB_003810"
     CDS             5229..5813
                     /gene="phnH"
                     /locus_tag="ACHKUB_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO6656771.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5813..6877
                     /gene="phnI"
                     /locus_tag="ACHKUB_003811"
     CDS             5813..6877
                     /gene="phnI"
                     /locus_tag="ACHKUB_003811"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO6656772.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6870..7715
                     /gene="phnJ"
                     /locus_tag="ACHKUB_003812"
     CDS             6870..7715
                     /gene="phnJ"
                     /locus_tag="ACHKUB_003812"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO6656773.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7712..8470
                     /gene="phnK"
                     /locus_tag="ACHKUB_003813"
     CDS             7712..8470
                     /gene="phnK"
                     /locus_tag="ACHKUB_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO6656774.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8581..9261
                     /gene="phnL"
                     /locus_tag="ACHKUB_003814"
     CDS             8581..9261
                     /gene="phnL"
                     /locus_tag="ACHKUB_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO6656775.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9258..10394
                     /gene="phnM"
                     /locus_tag="ACHKUB_003815"
     CDS             9258..10394
                     /gene="phnM"
                     /locus_tag="ACHKUB_003815"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO6656776.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10394..10951
                     /gene="phnN"
                     /locus_tag="ACHKUB_003816"
     CDS             10394..10951
                     /gene="phnN"
                     /locus_tag="ACHKUB_003816"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO6656777.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10938..11372
                     /gene="phnO"
                     /locus_tag="ACHKUB_003817"
     CDS             10938..11372
                     /gene="phnO"
                     /locus_tag="ACHKUB_003817"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO6656778.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHNAHRFYLREGYEQSHFRFTKAL"
     gene            11374..12132
                     /gene="phnP"
                     /locus_tag="ACHKUB_003818"
     CDS             11374..12132
                     /gene="phnP"
                     /locus_tag="ACHKUB_003818"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO6656779.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12279..12608)
                     /gene="yjdP"
                     /locus_tag="ACHKUB_003819"
     CDS             complement(12279..12608)
                     /gene="yjdP"
                     /locus_tag="ACHKUB_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO6656780.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12677..12994)
                     /locus_tag="ACHKUB_003820"
     CDS             complement(12677..12994)
                     /locus_tag="ACHKUB_003820"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO6656781.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITATEQ
                     RRN"
BASE COUNT         2884 a         3910 c         3631 g         2587 t
ORIGIN      
        1 gttgtttttt attagaataa ataacgtctt ccgatacgtt taaaacgtca gaaagataaa
       61 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
      121 agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
      181 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
      241 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
      301 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
      361 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
      421 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
      481 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
      541 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
      601 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
      661 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gctgaccgcg tcttatcagg
      721 cttccctctc atctgtataa atttcgaact acacttaact ggcttctctt aactgaggtc
      781 accatcatgc cgttaagtcc ctacctctct tttgccggta actgtgccga cgcaattgct
      841 tattatcaac gtacgttggg cgctgaactg ctctataaaa tcagcttcgg cgaaatgcca
      901 aaatcagcgc aggacagcgc cgagaactgc ccttccggaa tgcaatttcc cgataccgcc
      961 atcgcccacg ccaacgtgcg cattgccgga agcgacatca tgatgagcga tgctataccg
     1021 tcaggaaaag ccagctactc cggctttacg ctggtgctcg attcgcaaca ggtcgaagaa
     1081 ggaaaacgct ggtttgacaa tcttgccgct aacggaaaaa tcgaaatggc ctggcaggaa
     1141 actttctggg cgcatggctt tggcaaagtc accgataaat ttggcgtacc gtggatgatt
     1201 aatgtcgtca aacaacaacc aacgcaataa cccgccggga ggcccgccct cccgcactgt
     1261 catcgaattc ccgttaactc ttcatctgtt agtcactttt aattaaccaa atcgtcacaa
     1321 taatccgcca cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt
     1381 gtcgagaagc tcgccaaaac cttcaatcag catcaggcgc tgcatgcggt tgatctgaac
     1441 attcatcacg gtgaaatggt ggctctgctt gggccgtcgg gttccggcaa atccaccctt
     1501 ttacgtcact taagcggttt gattaccggc gataaatccg ccggcagcca tatcgagctg
     1561 ctgggccgca cagtccagcg cgaaggccgt ctggcgcgcg atatccgcaa aagccgcgcc
     1621 aacaccggct acatcttcca acaattcaac ctggtgaacc gcctgagcgt actggagaac
     1681 gtgctgattg gcgcgctcgg cagcacgccg ttctggcgca cctgttttag ctggttcacc
     1741 ggcgagcaga aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcattttgcc
     1801 catcagcgcg tttccaccct ctccggcgga cagcagcagc gtgtggcgat tgcccgcgcg
     1861 ctgatgcagc aggcgaaggt gattctggcc gatgaaccca tcgcctcgct ggacccggaa
     1921 tccgcccgca tcgtgatgga caccctgcgc gacatcaatc agaacgacgg catcaccgtg
     1981 gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg
     2041 cgccaggggc acgttttcta cgacggcagc agccaacagt ttgataacga acgttttgac
     2101 catctctacc gcagcattaa tcgcatcgaa gagaacgcga aagctgcctg acatccccat
     2161 cattgaggaa aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc
     2221 agcctcagca ccctgttaag cccggcacac gccgaagagc aggaaaaggc gctgaatttc
     2281 ggcattattt caacggaatc acagcaaaac ctgaaaccgc aatggacgcc attcttacag
     2341 gatatggaga agaagctggg cgtgaaggtg aacgccttct ttgccccaga ctacgcaggc
     2401 attatccagg gaatgcgctt caataaagtg gatatcgcct ggtacggcaa cctgtcggca
     2461 atggaagcgg tggatcgcgc caacggccag gtcttcgccc agacggtcgc ggcggatgga
     2521 tcgccaggtt actggagcgt gttgatcgtc aacaaagata gtccgatcaa caacctgaac
     2581 gatctgctgg cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctccacctct
     2641 ggcttcctcg tccccggtta ctacgtcttt gccaaaaaca atatctccgc cagcgacttt
     2701 aagcgcaccg tcaacgctgg gcatgaaacc aacgcgctgg ccgtcgccaa caagcaggtg
     2761 gatgttgcca ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccagagaag
     2821 ctgaaagaac tgaaggtgat ctggaagtcg ccgctgatcc caggcgatcc gatcgtctgg
     2881 cgcaagaatc tctccgaaac caccaaagac aagatctacg acttctttat gaactacgga
     2941 aaaacgccgg aagagaaagc ggtgctggaa cgcctgggct gggccccgtt ccgcgcctcc
     3001 agcgacctgc aactggtgcc gattcgccag ctcgcactgt ttaaagagat gcagggcgta
     3061 aaaagcaata aaggactgaa tgagcaggac aagctggcga aaaccaccga gattcaggcg
     3121 cagctggatg acctggaccg cctgaacaac gcgttaagcg cgatgagttc ggtgagtaaa
     3181 gcggtgcagt aatgtgctcg atcggtcccc tctcccttga gggagaggga gaaaacatta
     3241 aacggagcca aacatgcaaa ccatcaccat cgccccaccc aagcgtagct ggttctcgct
     3301 tctgagctgg gccgttgttc tcgccgtgct ggtcgtctcg tggcagggcg cggaaatggc
     3361 cccgcttacg ctgattaaag acggcggcaa catggcaacc ttcgctgccg acttcttccc
     3421 gcccgatttc agccagtggc aggattacct caccgaaatg gccgtcacgc tgcaaatcgc
     3481 cgtctggggc accgcgctgg cagtggttct ctccatcccc ttcggcctga tgagcgccga
     3541 aaacctggtg ccgtggtggg tttaccagcc cgttcgccgc ctgatggacg cctgccgcgc
     3601 cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc gtcggcctcg gcccgttcgc
     3661 tggcgtgctg gcgctgttta tccacaccac cggcgtgctc tccaagctgc tttccgaagc
     3721 ggtggaagcg attgaacccg gcccggtaga aggcattcgc gccaccggtg ccaacaagct
     3781 ggaagagatc ctttacggcg tgctgccaca ggtaatgccg ctgctgatct cctactccct
     3841 ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc ggcatggtcg gtgcaggcgg
     3901 gatcggcgtc accctgtggg aagcgattcg cggtttccag ttccagcaaa cctgcgccct
     3961 gatggtgctt atcatcgtca cggtcagcct gctggatttc ctctctcaac ggttgcgtaa
     4021 gcactttatc tgataagcga ggcattgata tctatgcact tgtctacaca tccgaccagc
     4081 tacccaacac gctatcaaga gatagccgca aaacttgagc aggagcttcg tcaacactac
     4141 cgctgcggcg actatcttcc cgccgagcag caactggcgg cgcgctttga ggtgaaccgc
     4201 cacaccctgc gccgcgccat cgaccaactg gtggaaaaag gctgggtaca gcgccgtcag
     4261 ggcgtcggcg tgctggtgct gatgcgcccg ttcgattacc cgctcaacgc ccaggcgcgt
     4321 tttagccaga acctgctgga ccagggtagc catcccacca gcgaaaaact gctgtcggta
     4381 ttgcgccccg cgtccggtca cgtcgctgac gcgctgggga ttaccgaggg cgagaacgtt
     4441 atccatttgc gcaccctgcg tcgcgtcaac ggcgtcgcgc tctgtttaat cgaccactac
     4501 tttgcggacc tcaccctctg gccgacactg caacgtttcg acagcggttc gctgcacgat
     4561 tttctgcgcg agcaaaccgg aattgcgctg cgccgcagcc agacgcggat cagcgcccgc
     4621 cgcgcccagg ccaaagagtg ccagcgcctt gaaattccga atatgtcgcc gctgctgtgc
     4681 gtgcgcaccc ttaaccaccg tgacggtgaa agcagcccgg cggagtactc cgtcagcctg
     4741 acgcgcgccg acatgattga attcactatg gagcactgaa tgcacgcaga taccgcgacc
     4801 cgccagcact ggatgtccgt actggcgcac agccaaccgg ctgaactggc agcccgcctg
     4861 aacgcgctga acatcaccgc cgactatgaa gtgatccgca ctgccgaaac tggcctggtg
     4921 cagattcagg cgcgaatggg cggtaccggc gaacgctttt ttgccggcga cgccacgctg
     4981 acccgcgccg ccgtacgcct gactgacggc accctcggct acagctgggt gctggggcgt
     5041 gataaacagc acgccgaacg ctgcgcgctg attgatgcgc tgatgcagca atctcgccat
     5101 tttcaaaact tatcagaaac ccttattgcc ccgctggacg ctgaccgtat ggcacgcatt
     5161 gccgcacgcc aggccgaagt gaacgccagc cgggtcgact tctttacgat ggttcgcgga
     5221 gacaacgcat gaccctggaa accgctttta tgcttcccgt gcaggatgcc cagcacagtt
     5281 ttcgtcgcct gttaaaggcg atgagcgagc cgggcgtgat tgtcgccctg catcagctca
     5341 aacgcggctg gcagccgctg aatatcgcca ccaccagcgt gctgctgacg ctggccgata
     5401 acgacacgcc ggtgtggctt gccacgccat taagtaacga tatcgtcaat cagagcctgc
     5461 gttttcatac caacgcgccg ctggtcagcc agccggaaca ggcgaccttc gcggtgacgg
     5521 atgaggcgat ttccagcgaa cagctcaacg ccctttccac cggcaccgcc gttgcgccgg
     5581 aagcgggcgc gacgctgatt ttacaggtcg ccagcctgag cggcggacgc atgttgcgtc
     5641 tcaccggcgc gggcattgcc gaagaacgaa tgatcgctcc gcagctgccg gagtgcattc
     5701 tgcacgaact caccgagcgc ccgcacccgt tcccgctcgg catcgacctg atcctgacct
     5761 gcggcgagcg cctgctggct gttccgcgaa ccactcatgt ggaggtgtgc tgatgtacgt
     5821 tgccgtgaaa gggggcgaaa aggcgatcga cgccgcccac gccctgcaag agagccgacg
     5881 ccggggcgat accgatttgc ccgaactgag cgtcgcccag attgaacagc agcttaacct
     5941 cgcggtagat cgcgtgatga cagaaggcgg cattgccgac cgcgaactgg cggcgctggc
     6001 gctgaaacag gccagcggcg ataacgttga agcaattttc ctgctgcgcg cctaccgcac
     6061 cacgctggcg aaactggcgg taagcgagcc gctcgacacc acagagatgc gtctcgaacg
     6121 gcgtatttcg gcggtttata aagacattcc cggcggccag ctgcttggcc caacctacga
     6181 ctacacccat cgcctgctcg atttcaccct gctggcaaac ggtgaagcgc cgacgctgac
     6241 caccgccgac agcgaacaac agccgtcgcc gcacgttttc agcctgctgg cgcgtcaggg
     6301 gctggcgaag tttgaagaag atagcggcgc acagccggat gacatcaccc gcacgccgcc
     6361 ggtttacccc tgctcacgct cctcccgcct gcaacaatta atgcgcgggg acgaaggcta
     6421 tttgctggcg ctggcctact ccacccagcg tggttacgga cgcaatcacc cgttcgcggg
     6481 cgagatccgc agtggttaca tcgacgtgtc gattgtgccg gaagagctgg gatttgcggt
     6541 aaacgtcggc gaactactga tgaccgagtg tgaaatggtc aacggtttta tcgacccgcc
     6601 ggatgagccg ccgcacttca cgcgcggcta cgggctggta ttcggcatga gcgagcgcaa
     6661 agcgatggca atggcgctgg tcgatcgtgc gttgcaggct ccggaatacg gcgagcacgc
     6721 gacaggcccg gcgcaggatg aagagtttgt gctggcacat gccgacaacg tcgaagccgc
     6781 aggctttgtc tcgcacctca aactccccca ctacgtcgat ttccaggccg aactggagct
     6841 actcaaacgt ctgcaacagg agaagaacca tggctaatct gagcggctac aactttgcct
     6901 acctcgacga gcagaccaaa cgcatgatcc gccgcgctat cttaaaagcg gtggcgatcc
     6961 ccggttatca ggtgccgttt ggcgggcgcg agatgccgat gccatacggc tggggaaccg
     7021 gcggcataca gctcaccgcc agcgtgattg gcgaaagcga cgtgctgaag gtgattgacc
     7081 agggtgcgga tgacaccacc aacgccgtgt cgattcgcaa cttctttaag cgcgtgaccg
     7141 gggtaaacac cactgaacgt acggacgatg cgacgcttat ccagacgcgt caccgcatcc
     7201 ccgaaacgcc gctgaccgaa gatcagatca ttatcttcca ggtgccaatc ccggaaccgc
     7261 tgcgctttat cgagccgcgc gaaacggaaa cccgcaccat gcacgcgctg gaagagtacg
     7321 gcgtgatgca ggtgaaactg tatgaagata tcgcccgctt cggtcatatc gccactacct
     7381 acgcctatcc ggtgaaggtg aacgggcgct acgtaatgga cccgtcgccg atcccgaaat
     7441 tcgataaccc aaaaatggac atgatgcccg ccctgcaact gttcggcgcg gggcgcgaga
     7501 agcgcatcta tgcggtgccg ccgtttaccc gcgtggaaag tctcgatttc gacgatcacc
     7561 cgttcaccgt tcagcagtgg gatgagccat gcgccatctg cggatcgacc cacagctatc
     7621 ttgatgaagt ggtgctggat gacgccggaa accgcatgtt tgtctgctcc gataccgatt
     7681 attgccgcca acagagcgag gcaaaaaacc aatgaatcaa ccgttacttt cggtcaataa
     7741 cctgacccac ctttacgcgc cgggcaaagg ctttagcgat gtctcttttg atttatggcc
     7801 gggggaagtg ctgggcattg tcggggagtc cggctccggg aagaccacgc tgctgaaatc
     7861 aatctccgcg cgcctgacgc cgcagcaggg ggagattcac tatgagagcc gttcgctgta
     7921 cgcaatgagc gaggccgacc gccgtcgcct gctgcgcacc gaatggggcg tagtgcatca
     7981 gcatccactc gacggcctgc gccgtcaggt ctcggcaggc ggcaatatcg gcgagcggct
     8041 gatggcgacc ggggcacgtc attacggcga tattcgtgcc accgcgcaga agtggctgga
     8101 agaggtggaa attcccgcca accggatcga cgacctgccg accacctttt ccggcggcat
     8161 gcagcaacgt ttgcagatcg cccgcaacct ggtgacgcat ccgaagctgg tgtttatgga
     8221 cgagccgacc ggcggactgg atgtgtcggt acaggcacgc ctgctcgacc tgctgcgcgg
     8281 cctggtggtg gagctgaacc tcgcggtagt gattgtcacc cacgatttag gcgtcgcccg
     8341 cctgctggcg gaccgtttgc tggtgatgaa gcaggggcaa gtggtggaga gtgggttaac
     8401 cgaccgcgtg ctcgacgacc cgcaccatcc gtatacacaa ctgctggtgt catcggtttt
     8461 gcagaattga gccggtgccg gatgcggcgt aaacgcctta tccggcctac aaatgcgctc
     8521 cccgtaggtc ggataagacg cgtcagcgtc gcatccgaca cccgaaccac gaggcgaaaa
     8581 atgattaacg tacaaaacgt cagtaaaacc ttcatcctgc accagcaaaa cggcgtgcgc
     8641 ctgcccgtct tacaacgtgc ctcgctcacc gtcaacgcgg gcgaatgcgt ggtgctccac
     8701 ggccattccg gcagcggcaa atcaaccttg ctgcgctcgc tgtacgccaa ctatctgccc
     8761 gacgaagggc aaatccagat caaacacggt gacgaatggg tcgatctggt caccgcgcca
     8821 gcgcgcaaag tggtggagat ccgcaaaacc accgtcggct gggtgagcca gtttctgcgc
     8881 gtcatcccgc gtatttcagc actggaagtg gtgatgcagc cgctgctcga taccggcgtt
     8941 ccgcgtgaag cctgcgccgc taaagccgcg cgtctgctca cccgcctgaa cgtgccggaa
     9001 cgcctgtggc acctggcgcc atcaaccttt tccggtggcg aacagcagcg cgtcaacatc
     9061 gcccgcggct ttatcgtcga ctaccccatt ctgctgctcg acgaacctac cgcctcgctg
     9121 gacgccaaaa acagcgccgc cgtagtcgag ctgattcgcg aagccaaaac ccgtggcgcg
     9181 gccatcgtcg gcatcttcca tgacgaagcc gtacgtaatg acgtcgccga ccgcttgcac
     9241 ccaatgggag cctctgcatg attatcaata acgtcaaact ggtgctggaa aacgaagtgg
     9301 tacacggttc gctggaggtg caggatggcg aaatccgcgc ctttgccgaa agccagagcc
     9361 gcctgccaga agcgatggac ggcgaaggtg gctggctact acccggcctg attgagctgc
     9421 ataccgacaa cctcgataaa ttcttcaccc cgcgcccgaa agtcgactgg cccgcccatt
     9481 cggcgatgag cagccacgac gcgctgatgg tggcaagcgg catcaccacc gtgctggacg
     9541 cggtggcgat tggcgacgtg cgcgacggcg gcgatcggct ggagaatctg gagaagatga
     9601 tcaacaccat cgaagagacg cagaaacgcg gcgtcaaccg cgccgagcac cgcctgcacc
     9661 tgcgctgcga actgccgcat cacaccacac tgccgctgtt tgaaaaactg gttcagcgcg
     9721 aaccggtgac gctggtgtcg ctgatggacc actcaccggg ccagcgccag ttcgctaacc
     9781 gcgagaagta tcgcgaatat tatcagggca aatactccct caccgatgcg cagatgcagc
     9841 agtacgaaga agagcaattg gcgctcgccg cacgctggtc gcagccgaat cgcgaatcca
     9901 tcgccgccct gtgccgcgcg cgacaaattg cgctcgccag ccacgatgac gccacacacg
     9961 cccacgttgc cgaatcccac caacttggca gcgtgatcgc cgaatttccc accacgttcg
    10021 aagcggcgga agcctcgcgt aagcatggca tgaacgtgct gatgggtgcg ccaaatatcg
    10081 tgcgtggcgg ctcgcactcc ggcaatgtgg cggccagtga actggcgcag cttggtctgc
    10141 tggatatcct ctcttccgac tactaccccg ccagcctgct ggatgcggcg ttccgcgtcg
    10201 ccgatgacga gagcaaccgc tttacgctac cgcaggcggt gaggctggtg accaaaaatc
    10261 cggcgcaggc gctgaatctt caggatcgcg gggtgattgg cgagggtaaa cgagctgacc
    10321 tggtgctggc gcatcgccag ggcaatcaca ttcatatcga ccacgtctgg cgtcagggta
    10381 aaagggtgtt ctgatgatgg gaaaactgat ttggttaatg gggccgtccg gctccgggaa
    10441 agacagcctg ctggcggaac tccgcctgcg ggaacaaacg cagttactgg tggcgcatcg
    10501 ctacatcacg cgcgacgcca gcgccggaag tgaaaaccat atcgccctga gtgagcggga
    10561 gttttttacc cgcgcggggc aaaacctgct ggccttaagc tggcacgcta acggtctgta
    10621 ttatggcgtc ggcatcgaga ttgacctctg gctgcacgct ggattcgacg tagtcgtcaa
    10681 cggttcacgc gcgcatttgc cgcaggcacg ggcgcgctat caatcggcgc tgctgcccgt
    10741 ctgtttacag gtttcgccgg agatcctgcg ccagcgcctg gaaaaccgtg gccgtgaaaa
    10801 cgccagtgaa attaacgccc gcctggcgcg cgccgcccgc tatactccac aggattgcca
    10861 tacgctcaac aatgacggca gcctgcgcca gtcggtcgac acgctgctga cgctgatcca
    10921 tcagaaggag aaacaccatg cctgcttgtg agcttcgccc ggccacgcag tacgataccg
    10981 acgcggttta cgcgctgatt tgtgagctaa aacaggcgga gtttgaccac catgcgtttc
    11041 gcgtgggctt taacgccaac ctgcgcgacc caaacatgcg ctaccatctg gcgctgctgg
    11101 atggcgaagt tgtcggcatg atcggcctgc atttgcagtt tcatctgcat catgtcaact
    11161 ggatcggcga aattcaagaa ctggtggtga tgccgcaggc gcgcggtctg aacgtcggca
    11221 gtaaattact ggcatgggca gaagaagaag cccgccaggc cggggccgaa atgaccgagc
    11281 tttccaccaa cgtgaagcgc cacaacgcac accgttttta cctgcgtgaa ggctacgagc
    11341 agagccactt ccgcttcacc aaggcgctgt aacatgagcc tgaccctcac gctcaccggc
    11401 actggcggcg cacagggcgt tccggcatgg ggctgcgagt gcgcggcctg cgccagagcg
    11461 cggcgctcgc cgcagtatcg ccgccagcca tgcagcggcg tagtgaagtt taacgacgcg
    11521 atcaccctga tcgacgccgg gcggcacgat ctcaccgatc gctggtcgcc tggatcgttc
    11581 cagcagtttt tgctgacgca ctaccatatg gatcacgtcc aggggctatt tccgctacgc
    11641 tggggcgtgg gcgatgtgat cccggtctac ggcccgccgg atgaacaggg ctgcgacgat
    11701 ctgtttaaac atccgggcct gcttgatttc agccacacag tggaaccgtt tgtggtgttt
    11761 gatttgcagg ggttgcaggt cacgccgctg ccgcttaacc actcaaaact gaccttcggt
    11821 tatctgctgg aaacggcaca tagccgggtg gcgtggctgt ctgacaccgc tggcctgccg
    11881 gaaaaaacgc tgaaattttt actcaataat catccgcagg tgatggtgat tgattgcagt
    11941 cacccgccgc gcgcggatgc accgcgtaat cactgtgatt taaataccgt gcttgcgctg
    12001 aatcaggtta tccgctcgcc acaggtgatt ctgacccata tcagccacca gtttgatgcg
    12061 tggctgatgg aaaacgcact accgtcaggg tttgaggtgg ggtttgatgg gatggagatt
    12121 ggggtggcgt gatgagaggg aatgtgcgcg ctggccccct caccctaacc ctctccccag
    12181 aggggcgagg ggaccgattg tgctcgatat tgaatattgc gctcgttttc tccctctccc
    12241 cattggggtg aggggcgatg cctgctccat acccaacctc atcgcccata ctcatcttcc
    12301 attcttcgct cttcatcctc cagttgccga cgctcctgat caagctggcg ctggcgatcg
    12361 tccagctgcc tgcggcgatc ttcaaactgg cggcggcggt cgtcatattg tctgcgccga
    12421 tcgtcgctca cttcacgctg ccagccgtcg tcgcgcgaat cttcatagtc tcgcccacgg
    12481 tcagggttat aagcgtcatt aatcgcctgc tgaatattac caatagtatc gtcgataata
    12541 tcagcctggg ccggaacggt ggacagcgtg agcagggtga ataaaagaaa tagcggaaag
    12601 cgtttcatta gccaacctca aaaagaaact ctatccacat taatcattac tcatccatgc
    12661 aagtagtgga tgaatctcaa tttctccgct gctctgttgc cgtaatcgcc tccacgcgtt
    12721 gttgatgacg accgccttcg tactgtgcgc ccagccacgc atccacaatc atttttgcca
    12781 gttcgaggcc aaccactcgt gaaccaaaag ccagtgcaac ttcactggca taatgcggat
    12841 aatcagtgcg ctctgacgac ctggtgtctt tatcaatcac ctcactcatt gaccatgaat
    12901 aaaaggctgg cacagcaacg cagaatcctg cggtgccagc gacatgaccg cccattgctc
    12961 cggtacgttt tttaaaaaga aacaccggtt gcattgataa attgtgctca gt
//
LOCUS       DAZFRN010000135        12985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFRN010000135 DAZFRN010000000
VERSION     DAZFRN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.137"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            214..1323
                     /gene="livK"
                     /locus_tag="ACHKUB_003821"
     CDS             214..1323
                     /gene="livK"
                     /locus_tag="ACHKUB_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO6656782.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1371..2297
                     /gene="livH"
                     /locus_tag="ACHKUB_003822"
     CDS             1371..2297
                     /gene="livH"
                     /locus_tag="ACHKUB_003822"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO6656783.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2294..3571
                     /gene="livM"
                     /locus_tag="ACHKUB_003823"
     CDS             2294..3571
                     /gene="livM"
                     /locus_tag="ACHKUB_003823"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO6656784.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQILRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3568..4335
                     /gene="livG"
                     /locus_tag="ACHKUB_003824"
     CDS             3568..4335
                     /gene="livG"
                     /locus_tag="ACHKUB_003824"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO6656785.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4337..5050
                     /gene="livF"
                     /locus_tag="ACHKUB_003825"
     CDS             4337..5050
                     /gene="livF"
                     /locus_tag="ACHKUB_003825"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO6656786.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5446..6762
                     /gene="ugpB"
                     /locus_tag="ACHKUB_003826"
     CDS             5446..6762
                     /gene="ugpB"
                     /locus_tag="ACHKUB_003826"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO6656787.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6860..7747
                     /gene="ugpA"
                     /locus_tag="ACHKUB_003827"
     CDS             6860..7747
                     /gene="ugpA"
                     /locus_tag="ACHKUB_003827"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO6656788.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7744..8589
                     /gene="ugpE"
                     /locus_tag="ACHKUB_003828"
     CDS             7744..8589
                     /gene="ugpE"
                     /locus_tag="ACHKUB_003828"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO6656789.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8591..9661
                     /gene="ugpC"
                     /locus_tag="ACHKUB_003829"
     CDS             8591..9661
                     /gene="ugpC"
                     /locus_tag="ACHKUB_003829"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO6656790.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9658..10401
                     /gene="ugpQ"
                     /locus_tag="ACHKUB_003830"
     CDS             9658..10401
                     /gene="ugpQ"
                     /locus_tag="ACHKUB_003830"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6656791.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10388..10828)
                     /locus_tag="ACHKUB_003831"
     CDS             complement(10388..10828)
                     /locus_tag="ACHKUB_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO6656792.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10948..12690
                     /gene="ggt"
                     /locus_tag="ACHKUB_003832"
     CDS             10948..12690
                     /gene="ggt"
                     /locus_tag="ACHKUB_003832"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO6656793.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWHALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12728..>12985)
                     /locus_tag="ACHKUB_003833"
     CDS             complement(12728..>12985)
                     /locus_tag="ACHKUB_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO6656794.1"
                     /translation="KANHYLDDADLPVVITLHGRFSQGWYFCFEAREFLETGDEAARL
                     AGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2869 a         3331 c         3731 g         3054 t
ORIGIN      
        1 aggcactttt ttctgtttat ctatcaataa attcagaata ttatctgttc ttaatcgact
       61 gaaaaatagg gattttaatc actattatca caaaatactg cgctaaaccc ttaatcagac
      121 aggcaaaaac agtgcagtat aaaaaaagaa cagtctgatt tgttaacaca taaaaacaaa
      181 gcaacacaac atcacgaatg gggatttttg actatgaaac ggaatgcgaa aactatcatc
      241 gcagggatga ttgcactggc aatttcacac accgctatgg ctgacgatat taaagttgcc
      301 gttgtcggcg cgatgtccgg cccgattgcc cagtggggcg atatggaatt taacggcgcg
      361 cgtcaggcga ttaaagacat taatgccaaa gggggaatta aaggcgataa gctggtgggc
      421 gtggaatatg acgacgcctg cgacccgaaa caagccgttg cggtcgccaa caaaatcgtt
      481 aacgacggca ttaaatacgt tattggtcat ctgtgttctt cttctaccca acctgcgtca
      541 gatatctacg aagacgaagg tattctgatg atctcgccgg gagcgaccaa cccggagctg
      601 acccaacgcg gttatcaaca cattatgcgt actgccgggc tggactcttc ccaggggcct
      661 acggcggcaa aatacattct tgagacggtg aagccccagc gcatcgccat cattcacgac
      721 aaacaacagt atggcgaagg gctggcgcgt tcggtgcagg acgggctaaa agcggctaac
      781 gccaacgtcg tcttcttcga cggtattacc gccggggaga aagatttctc cgcgctgatc
      841 gcccgcctga aaaaagaaaa catcgacttc gtttactacg gcggttacta cccggaaatg
      901 gggcagatgc tgcgccaggc ccgttccgtt ggcctgaaaa ctcagtttat ggggccggaa
      961 ggtgtgggta acgcatcatt gtcgaatatt gccggtgatg ctgccgaagg catgttggtc
     1021 actatgccaa aacgctatga ccaggatccg gcaaaccagg gcatcgttga tgcgctgaaa
     1081 gcagacaaga aagatccgtc cgggccttat gtctggatca cctacgcggc ggtgcaatct
     1141 ctggcgactg cccttgagcg tactggcagc gatgagccgc tggcgctggt gaaagattta
     1201 aaagctaacg gtgcaaacac cgtgattggg ccgctgaact gggatgaaaa aggcgatctt
     1261 aagggatttg attttggtgt cttccagtgg cacgccgacg gttcatccac ggcagccaag
     1321 tgatcatccc accgcccgta aaatgcgggc gggtttagaa aggttacctt atgtctgagc
     1381 agtttttgta tttcttgcag cagatgttta acggcgtcac gctgggcagt acctacgcgc
     1441 tgatagccat cggctacacc atggtttacg gcattatcgg catgatcaac ttcgcccacg
     1501 gcgaggttta tatgattggc agctacgtct catttatgat catcgccgcg ctgatgatga
     1561 tgggcattga taccggctgg ctgctggtgg cggcgggatt cgtcggcgca atcgtcattg
     1621 ccagcgccta cggctggagt atcgaacggg tagcctatcg cccggtgcgt aactctaagc
     1681 gcctgattgc gctcatctcg gcaatcggca tgtccatctt cctgcaaaac tacgttagcc
     1741 tgaccgaagg ttcgcgtgac gtggcgctgc ccagtctgtt taacgggcag tgggtggtgg
     1801 gccatagcga aaacttctct gcctctatta ctaccatgca ggcggtgatt tggattgtta
     1861 ccttcctcgc catgctggcg ctgacgattt tcattcgcta ttcccgtatg gggcgcgcct
     1921 gtcgtgcctg cgcggaagat ctgaaaatgg cgagcctgct tggcatcaac accgaccggg
     1981 tgattgcgct gacctttgtg attggcgcgg cgatggcggc ggtggcgggt gtgctgctcg
     2041 gtcagttcta cggtgtgatt aacccctaca tcggctttat ggccgggatg aaagccttta
     2101 ccgcggcggt gctcggcggg attggcagca ttccgggggc gatgattggc gggctgatcc
     2161 tggggattgc ggaggcgctc tcttctgcct atctgagtac ggaatataaa gatgtggtct
     2221 catttgccct gctgatcctg gtactgctgg tgatgccgac cggtattctg gggcgcccgg
     2281 aggtagagaa agtatgaaac cgatgcatat tgcaatggcg ctgctctctg ccgcgatgtt
     2341 ctttatcctc gctggcgtct ttatgggcgt acagctggag ctggacggca ccaaactggt
     2401 ggtcgacacg gcttcggatg tccgttggca gtgggtgttt atcggcacgg cggtggtctt
     2461 tttcttccag attttgcgac ctgctttcca gaaagggttg aaaagcgttt ccgggccgaa
     2521 gtttattctg cccgcgattg atggctccac ggtgaagcag aaactgttcc tcgtggcgct
     2581 gttggtgctt gcggtggcgt ggccgtttat ggtttcacgc gggacggtgg atatcgccac
     2641 cctgaccatg atctacatta tcctcggcct cgggctgaac gtggtggtag ggctttccgg
     2701 cctgctggtg ctggggtacg gcggttttta cgccatcggc gcttacactt ttgcgctgct
     2761 caatcactat tacggcttgg gtttctggac ctgtctgccg attgccgggt taatggcagc
     2821 ggcggcgggc ttcctgctcg gctttccggt gctgcgtctg cgtggtgatt atctggcgat
     2881 cgttaccctc ggcttcggcg aaattgtgcg catattgctg ctcaataaca ccgaaattac
     2941 cggcggcccg aacggaatca gtcagatccc gaaaccgact ttcttcggcc ttgagttcag
     3001 ccgtaccgcc cgtgaaggcg gttgggacac gttcagtaat ttctttggcc tgaaatacga
     3061 tccctccgat cgcgtcatct tcctgtatct ggtggcgttg ctgctggtgg tgctaagcct
     3121 gtttgtcatt aaccgcctgc tgcggatgcc gctggggcgt gcgtgggaag cgttgcgtga
     3181 agatgagatc gcctgccgtt cgctgggctt aagcccgcgt cgtatcaagc tgaccgcttt
     3241 taccatcagt gccgcgtttg ccggttttgc cggaacgctg tttgctgcgc gccagggctt
     3301 tgtcagcccg gaatccttca cctttgctga atcggcgttt gtactggcga tagtggtgct
     3361 cggcggtatg ggatcgcagt ttgcggtgat tctggcggca attttgctgg tggtgtcgcg
     3421 cgagttgatg cgtgatttca acgaatacag catgttaatg ctcggtggtt tgatggtgct
     3481 gatgatgatc tggcgtccgc agggcttgct gcccatgacg cgcccgcaac tgaagctgaa
     3541 aaacggcgca gcgaaaggag agcaggcatg agtcagccat tattatctgt gaacggcctg
     3601 atgatgcgct tcggcggtct gctggcggtg aacaacgtca atcttgaact gtacccgcag
     3661 gagatcgtct cgttaatcgg ccctaacggt gccggaaaaa ccacggtttt taactgcctg
     3721 accggtttct acaaacccac cggcggcacc attttactgc gcgatcagca tcttgaaggt
     3781 ttaccggggc agcaaattgc ccgcatgggc gtggtgcgca ccttccagca tgtgcgtctg
     3841 ttccgtgaaa tgacggtaat tgaaaacctg ctggtggcgc agcatcagca actgaaaacc
     3901 gggctgttct ctggcctgtt gaaaacgcct tctttccgtc gcgcccagag cgaagcgctc
     3961 gaccgcgccg ccacctggct tgagcgcatt ggtttgctgg aacacgccaa ccgtcaggcg
     4021 agtaacctgg cctatggtga ccaacgccgt ctggagattg cccgctgcat ggtgacgcag
     4081 ccggagattt taatgctcga cgaaccggcg gcaggtctta acccaaaaga aaccaaagag
     4141 ctggatgagc tgattgccga actgcgcaat catcacaaca ccactatctt gttgattgaa
     4201 cacgatatga agctggtgat ggggatttca gaccgaattt atgtggtcaa tcaggggacg
     4261 ccactggcaa acggtacgcc ggagcaaatc cgtaataacc cggacgtgat ccgtgcctat
     4321 ttaggtgagg cataagatgg aaaaagtcat gttgtccttt gacaaagtca gcgcccacta
     4381 cggcaaaatc caggcgctgc atgaggtgag cctgcatatc aatcagggcg agattgtcac
     4441 cctcattggc gcgaacgggg cggggaaaac caccttgctc ggcacgttat gcggcgatcc
     4501 gcgcgctacc agcgggcgaa ttgtgtttga tgataaagac attaccgact ggcagacggc
     4561 gaaaatcatg cgcgaagcgg tagcgattgt cccggaaggg cgtcgcgtct tctcgcggat
     4621 gacggtggaa gagaacctgg cgatgggcgg cttttttgcc gaacgcgacc agttccagga
     4681 gcgcataaag tgggtgtatg aactgtttcc acgtctgcat gagcgccgtg ttcagcgggc
     4741 aggcaccatg tccggcggtg agcaacaaat gctggcgatt ggtcgcgcgc tgatgagcaa
     4801 cccgcgtttg ctgctacttg atgagccatc gctcggtctt gcgccgatta tcatccagca
     4861 aattttcgac accatcgagc agctgcgcga gcaggggatg actatcttcc tcgtcgagca
     4921 gaacgccaac caggcgctaa agctggcgga tcgcggctac gtgctggaaa acggccatgt
     4981 ggtgctctcc gataccggcg acgcgctgct ggcgaacgaa gcagtgagaa gtgcgtattt
     5041 aggcgggtaa taacacgttg attgataagg agtcaaaaga ctcctttgag acaggtgaca
     5101 aatgtaaaat tgcctgatgc gctgcgctta tcaggcctac tgggtgagtt gcaatatatt
     5161 gaatttgcac gatcttgtag gccggataag gcgtttacgc cgcatccggc atgaaacgat
     5221 gagtaatctg tagagtttga ttcagacctt ctattttccg cttatccgtg ccccatctcc
     5281 cattttccct cacccacgcc gtcaccgcct tgtcatcttt ctgacacctt actatcttac
     5341 aaatgtaaca aaaaagttat ttttctgtaa ttcgagcatg tcatgttacc ccgcgagcat
     5401 aaaacgcgtg aattcgcgca ttcggtacaa caagagagat aaacgatgaa accgttacat
     5461 tatacagctt cagcactggc gctcggactg gcgttaatgg ggaatgcaca ggcagtgacg
     5521 accattccgt tctggcattc tatggaaggg gaactgggta aagaggtgga ttctctggcc
     5581 caacgtttta acgccgaaaa tccggattac aaaattgtac cgacctataa aggcaactac
     5641 gaacagaatt taagcgcggg gattgccgca tttcgtaccg gcaacgctcc ggctattttg
     5701 caggtttatg aagttggcac cgccaccatg atggcatcga aagccattaa accggtatat
     5761 gacgtgttta aagaggcggg gattcaattc gatgagtcgc agtttgtgcc gacggtttcc
     5821 ggttactact ccgacagcaa aacgggccac ttactctccc agccattcaa cagctcgacc
     5881 cccgttctct attacaacaa agacgccttc aagaaagcag gattagaccc ggaacaaccg
     5941 ccgaaaacct ggcaggatct ggcggactat gccgcgaaac tgaaagcctc cggtatgaag
     6001 tgcggctacg ccagcggctg gcagggctgg atccaactgg aaaactttag cgcctggaac
     6061 ggtctgccgt ttgccagcaa aaacaacggc tttgacggca cggacgcggt gctggagttc
     6121 aacaagccgg agcaggtgaa acacatcgcc atgctcgagg agatgaacaa gaagggcgac
     6181 ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt tctataacgg tgattgcgcg
     6241 atgaccaccg cctcttccgg ttctctcgct aatattcgtg agtacgccaa atttaactat
     6301 ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg caccgcaaaa cgccattatc
     6361 ggcggggcca gcctgtgggt gatgcagggc aaaaataaag aaacgtatac cggcgtggcg
     6421 aagttcctcg acttcctcgc gaagccagaa aacgctgccg agtggcatca gaaaaccggt
     6481 tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg agcagggctt ttacgagaaa
     6541 aacccagggg cggataccgc gacgcgtcag atgctgaata agccgccgtt gccgttcacc
     6601 aaagggctgc gtctgggcaa catgccgcag atccgcgtga ttgtggatga agagctggag
     6661 agcgtgtgga ccggtaagaa gacaccacag caggcactgg ataccgccgt tgagcgtgga
     6721 aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt aatcagtgta atgtcggatg
     6781 cgtttcgctt atctgacctg gcatcgcgtg taggccggat aagcgaagcg catccggcac
     6841 agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg ttccgctcgc gctggctgcc
     6901 ttatctgctg gtcgcgccgc agctcatcat caccgttatc ttttttatct ggcctgcggg
     6961 cgaagcgttg tggtactcgc tacaaagcgt cgatccgttt ggtttctcca gccagtttgt
     7021 cggcctggat aacttcgtca cgctgttcca tgacagctac tatctcgact ccttctggac
     7081 gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg ctggtatcgc tgttctttgc
     7141 ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat caaaccttaa tgttgctgcc
     7201 ttacgccgtg gctcccgccg ttgccgccgt attgtggatc ttcctgttta accccggtcg
     7261 cgggctgatc acccattttc tcgcggagtt cggctacgac tggaaccacg cgcaaaacag
     7321 cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg aagcaaatca gctacaactt
     7381 tctgttcttc tatgccgcgc tgcaatccat tccccgttcg ttgatcgaag ccgcagccat
     7441 cgacggtgca gggccgattc gccgcttctt taagattgcg ctgccgctta tcgccccggt
     7501 gagtttcttc ctgctggtag tgaacctggt gtatgccttc ttcgacacct tcccggtgat
     7561 cgacgccgcc acgtccggcg ggccggttca ggccaccacg acgctgattt ataagatcta
     7621 ccgcgaaggt tttaccggac tggatctggc ttcgtctgcc gcacagtcgg tggtgttgat
     7681 gttcctcgtc atcgtgctga cggtggtgca gttccgctat gttgaaagca aggtgcgtta
     7741 ccaatgattg agaaccgtcc gtggctgacg atattcagcc ataccatgct gatcctcggg
     7801 atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg cggcgacgct ggataaacag
     7861 gccgtctatg ccgcgccgat gacgctcatc cccggcacac atctgctgga aaacatccac
     7921 aacatctggg tgaacggggt aggcacgaat agcgcgccgt tctggcggat gttgcttaac
     7981 agctttgtga tggcgttcag cattacgctc ggcaaaatta ccgtctcgat gctctcggca
     8041 tttgccattg tctggtttcg ttttccgcta cgtaacctct tcttctggat gatttttatc
     8101 accctgatgc tgccggttga agtacgtatc ttcccgacgg tggaagtcat cgccaacctg
     8161 cagatgctcg acagctacgc cggtttaacg ctgccgctga tggcctcggc gaccgctact
     8221 ttcctgttcc gccagttctt tatgacgctg ccggatgagc tggtggaagc cgcgcggatc
     8281 gacggcgcat cgccaatgcg cttcttttgc gacatcgttt ttccgctctc caaaactaat
     8341 ctggcggcgc tgtttgtgat cacctttatc tacggctgga atcagtattt gtggccgttg
     8401 ttgattatta ccgatgtgga tctcggcacc accgtggcag ggatcaaagg gatgatcgct
     8461 acaggcgaag gcaccacgga atggaactca gtgatggcgg cgatgttgtt aacgcttatc
     8521 cctccggtgg tgattgtttt agtgatgcag cgtgccttcg tgcgcggcct ggtcgatagt
     8581 gagaaataag atggcaggac tgaaattaca ggcagtaacc aaaagctggg atggtaaaac
     8641 ccaggtcatt aaaccgctga cccttgatgt ggcggatggc gaatttatcg tgatggtcgg
     8701 gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt gccgggctgg agcgggtgac
     8761 agaaggcgat atctggatca acgaccagcg cgtgactgaa atggagccaa aagatcgcgg
     8821 gattgcgatg gtgttccaga actacgcgct ttatccgcat atgagtgtcg aagaaaacat
     8881 ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa attgccgagc gcgttaaaga
     8941 agcggcgcgc attctggagc tggacggtct gctcaaacgt cgcccgcgcg agctttccgg
     9001 cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg cgcgatccgg cggtgttcct
     9061 gtttgatgag ccgctctcta acctcgatgc caagctgcgc gtgcagatgc gtcttgaact
     9121 gcaacagttg caccgtcgcc tgaaaacgac ttcactctac gttactcacg atcaggttga
     9181 agcgatgacg ctcgcccagc gagtaatggt gatgaacggc ggcgttgccg aacagattgg
     9241 cacaccagtt gaagtctacg aaaagcccgc cagcctgttt gtagcgagtt ttatcggcag
     9301 tccggcgatg aacctgctga caggccgcgt gaataccgaa ggcacgcatt tcgaactgga
     9361 cggcggtatc gtgctaccgc taaacggtgg ctaccgtcag tatgccgggc gtaaaatgac
     9421 tctcggcatt cgcccggaac atattgcgct aagctcgcaa gcagaaggtg gcgtaccgct
     9481 ggtgatggat acgctggaga tcctcggcgc agataacctg gcgcacggac gctggggcga
     9541 gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg acggcaggca gcacgctgtg
     9601 gctgcatctg gcggaaaatc agctgcatct ttttgatggt gaaacaggac aacgagtatg
     9661 agtaactggc cttatccccg catcgtcgct catcgtggcg gcggtaagct ggcccccgaa
     9721 aacaccctgg cggcaatcga cgttggggca aaatacggtc ataagatgat cgaatttgac
     9781 gcgaagttat cgaaagatgg cgagatcttc ctgctccatg acgacaatct cgaacgcacc
     9841 agcaacggct ggggcgtcgc gggtgaactg aactggcagg atttactgcg cgtggatgcg
     9901 ggcagttggt acagcaaagc gtttaaaggt gagccgctgc cgttgctttc gcaggtggcg
     9961 gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa tcaaacccac cactggcact
    10021 ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc aactgtgggc cggtatgacg
    10081 ccgccgctgc tgtcatcgtt tgagattgat gctttagaag ctgcacagct ggcggcaccg
    10141 gaactgccgc gtggtttatt gcttgatgag tggcgcgacg actggcgcga actgaccgcg
    10201 cggctgggct gcgtctctat tcatctcaat cataagttac tcgataaagt gcgagtgatg
    10261 cagttgaaag acgccggact gcggattctg gtttataccg tcaataaacc ccagcgcgcg
    10321 gcagagttgc tgcgctgggg cgtggattgc atctgtaccg atgcgattga cgtgattggt
    10381 ccgaacttta cggcccaata gttttcaacg gaatgtcagg ctgcggcgtg ctgggctgat
    10441 tcagcatgtc gccgtttctc ctctccggca gcatatgctg ctgattcaac gaactatccg
    10501 gattacggtt gctgtttaac atcccgccgt tggtgttggg cagcatttgc tgccgggcgg
    10561 gatttcgctc ccccggctgt gactgcaaca cccgctgaga attattgttt atctggtttt
    10621 ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg ctgattcagc atcccttttt
    10681 gctggatttg ctgagtctgc atctgggttt gcatccgctg ctggctgggt atctgatacc
    10741 ccggctggtt agggttgttc agagtattaa tgggctgtgc aaagccgaca aacggcagga
    10801 gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc tctgaagata tcctttaagt
    10861 ttactcgctt cccgacaaaa cgatgattaa ttcagagtta tataccaggc ttagctgggg
    10921 ttgcccctta atctctggag aataacgatg ataaaaccga cgtttttacg ccgggtggcc
    10981 attgctgctc tgctctcagg aagttgtttt agcgccgccg ccgcgcctcc tgcgccgccc
    11041 gtctcgtatg gtgtggagga agatgtcttc cacccggtac gcgcgaaaca gggaatggta
    11101 gcgtctgtgg acgccactgc cactcaggtg ggggtggata ttctcaagga gggcgggaat
    11161 gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg taacgcatcc gcaggcaggg
    11221 aatctgggcg gtggtggttt tatgttaatc cgctcgaaaa atggcaatac cacggctatc
    11281 gatttccgcg aaatggcacc cgccaaagcg acccgcgata tgttcctcga tgatcagggc
    11341 aacccggaca gcaaaaaatc actcacttcg catctggctt ccggcacacc gggtacggta
    11401 gcaggtttct cgctggcgct ggataaatac ggcaccatgc cgctgaacaa agtcgtgcag
    11461 cccgcgttta aactggcacg cgatggtttt atcgttaacg atgctttggc agacgatctc
    11521 aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca gtaaagctat cttctggaaa
    11581 gagggcgagc cgctgaaaaa gggcgacacg ctggtgcagg cgaacctggc aaagagcctg
    11641 gagatgattg ctgaaaacgg cccggacgaa ttctataaag gcacgattgc ggaacagatc
    11701 gcccaggaga tgcagaaaaa cggtggcttg atcactaaag aagatttagc agcctataaa
    11761 gcggtcgaac gcactccgat aagcggcgat tatcgcgggt atcaggttta ctccatgcca
    11821 ccgccatcct ccggcgggat ccatatcgta caaatcctca atattctgga aaacttcgat
    11881 atgaagaaat acggctttgg cagcgccgat gcgatgcaaa tcatggcaga agcggagaaa
    11941 tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg attttgtcaa agtaccgtgg
    12001 catgcgctga ccaataaagc ctatgccaaa tctattgccg atcaaattga tatcaataaa
    12061 gcgaagccat ccagcgaaat tcgccccggc aagcttgcgc cttatgagag taatcaaact
    12121 acccattact cagtggtgga taaagatggt aacgcggtgg cggtgaccta tacgctgaac
    12181 accaccttcg gtacgggcat tgtcgcgggc gagagcggta ttctgcttaa taaccagatg
    12241 gatgatttct ccgccaaacc gggcgtaccg aacgtttacg ggctggtggg cggtgatgcc
    12301 aacgccgtcg ggccgaacaa acgcccgctg tcgtcgatgt cgccgaccat tgtggtgaaa
    12361 gacggtaaaa cctggctggt taccggtagc ccaggcggta gccggatcat cactacagtg
    12421 ctgcaaatgg tggtgaatag catcgattat ggcatgaacg tcgccgaagc gaccaatgcg
    12481 ccgcgtttcc accatcagtg gttgccggac gagctgcgtg tcgaaaaagg gtttagcccg
    12541 gatacgctca agctgctgga agcaaaaggt cagaaagtgg cgctgaaaga ggcgatgggc
    12601 agtacacaaa gcattatggt tgggccggac ggtgagttgt acggcgcatc cgacccgcgc
    12661 tcggtggatg atttaacggc ggggtactaa ggttagcggc cctcttcgtg ggaagagggc
    12721 tattttgtca gggcaagccg aaggtagcct tttttatttc gtaatcctgt agatattctt
    12781 ccagcggctt tgccgttccc agagaatgaa tttcaccact gtctttatca ataataaaag
    12841 gtgcgttacc agctaagcgc gcggcctcat ctccagtttc gagaaattct cgtgcttcga
    12901 aacagaaata ccagccctgg ctaaagcgtc catgtagagt aatgacgacc gggagatctg
    12961 catcatcaag gtaatggttc gcttt
//
LOCUS       DAZFRN010000136        12861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFRN010000136 DAZFRN010000000
VERSION     DAZFRN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.138"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            170..751
                     /gene="mdaB"
                     /locus_tag="ACHKUB_003834"
     CDS             170..751
                     /gene="mdaB"
                     /locus_tag="ACHKUB_003834"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO6656795.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            782..1096
                     /locus_tag="ACHKUB_003835"
     CDS             782..1096
                     /locus_tag="ACHKUB_003835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO6656796.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1144..3036)
                     /gene="parE"
                     /locus_tag="ACHKUB_003836"
     CDS             complement(1144..3036)
                     /gene="parE"
                     /locus_tag="ACHKUB_003836"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO6656797.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3065..3646)
                     /gene="yqiA"
                     /locus_tag="ACHKUB_003837"
     CDS             complement(3065..3646)
                     /gene="yqiA"
                     /locus_tag="ACHKUB_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO6656798.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3646..4473)
                     /gene="cpdA"
                     /locus_tag="ACHKUB_003838"
     CDS             complement(3646..4473)
                     /gene="cpdA"
                     /locus_tag="ACHKUB_003838"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO6656799.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADKRFQPDTASEGY"
     gene            complement(4498..4920)
                     /locus_tag="ACHKUB_003839"
     CDS             complement(4498..4920)
                     /locus_tag="ACHKUB_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO6656800.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4921..5550)
                     /gene="nudF"
                     /locus_tag="ACHKUB_003840"
     CDS             complement(4921..5550)
                     /gene="nudF"
                     /locus_tag="ACHKUB_003840"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO6656801.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5755..7236
                     /gene="tolC"
                     /locus_tag="ACHKUB_003841"
     CDS             5755..7236
                     /gene="tolC"
                     /locus_tag="ACHKUB_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO6656802.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPIVQQTSARTTTSNGHNPFRN"
     gene            7384..8055
                     /locus_tag="ACHKUB_003842"
     CDS             7384..8055
                     /locus_tag="ACHKUB_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO6656803.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8061..9221
                     /locus_tag="ACHKUB_003843"
     CDS             8061..9221
                     /locus_tag="ACHKUB_003843"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6656804.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9259..10047)
                     /gene="ygiD"
                     /locus_tag="ACHKUB_003844"
     CDS             complement(9259..10047)
                     /gene="ygiD"
                     /locus_tag="ACHKUB_003844"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO6656805.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10190..10963
                     /gene="zupT"
                     /locus_tag="ACHKUB_003845"
     CDS             10190..10963
                     /gene="zupT"
                     /locus_tag="ACHKUB_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO6656806.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11021..11191)
                     /gene="yqiD"
                     /locus_tag="ACHKUB_003846"
     CDS             complement(11021..11191)
                     /gene="yqiD"
                     /locus_tag="ACHKUB_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO6656807.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11452..12105)
                     /gene="ribB"
                     /locus_tag="ACHKUB_003847"
     CDS             complement(11452..12105)
                     /gene="ribB"
                     /locus_tag="ACHKUB_003847"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO6656808.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDLI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12209..12357)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12479..12769
                     /gene="ubiK"
                     /locus_tag="ACHKUB_003848"
     CDS             12479..12769
                     /gene="ubiK"
                     /locus_tag="ACHKUB_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO6656809.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3238 a         3449 c         3151 g         3023 t
ORIGIN      
        1 aatggctatc agtattgtca ttttcagaaa atatttatcc tgcatcggtg agtcagagct
       61 agatcagact tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt tcaccgtacc
      121 gctctgtgac gtactatagt cgacaaacgt ctcaccttga ggttaaaaaa tgagcaacat
      181 cctgattatc aacggcgcga aaaaattcgc ccactccaat ggtcaactga acgacaccct
      241 gaccgaagtc gcggatggca cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc
      301 cgacagcgac tacgatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat
      361 ctggcagatg ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat atattgatga
      421 tgtattcacc gaaggtcatg gaacgctgta tgccagcgat ggccgtaccc gcaaagatcc
      481 gtcaaaaaaa tacggttccg gcggcctggt acagggcaaa aaatatatgc tttctctgac
      541 ctggaacgca ccaatggaag ccttcaccga aaaagatcaa ttcttccacg gcgttggcgt
      601 cgacggtgtg tatctgccgt tccataaagc aaaccaattc cttggcatgg aaccgctgcc
      661 gacatttatc gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata
      721 tcgcaagcat cttgtggaaa tttttggtta actagagctc aggctttaga aggagttaac
      781 catgcttacc gtaatcgcag aaatccgtac tcgtcctggt caacatcacc gtcaggcggt
      841 attggatcag tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta
      901 tgcgccaatg gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat
      961 cgtgatgatt gagcagtggg aaagcatcgc gcatcttgaa gcgcatctgc aaaccccgca
     1021 catgaaggcg tacagcgaag ccgtaaaagg cgacgtgctg gagatgaata tccgcatcct
     1081 gcaacctggg atttaaacct gccttgtttg cccggccatc ctgaccgggc aatgttcttt
     1141 cctttaaacc tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc
     1201 cgagcgtttc ttcgccagca gcatatccat catcgcgtca gtacgctgat cgtcttcatc
     1261 atcgatagtc aactgcacca gacggcgagt gttcggatca agcgtggttt cacgcaattg
     1321 catcgggttc atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg
     1381 ttttaattgc tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt
     1441 cccgagatca atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa
     1501 cgcgcggaag tgttttacga acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga
     1561 gtccgcatcc gcgaggatac agattttgcc ataacgaagc tggctcagat cgtcgctgtc
     1621 aggatcgata ccgatcgcta ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc
     1681 ggaagaaact tcccaggtgt taaggatctt acctttcagt ggcatgatcg cctgatattc
     1741 gcgatcgcgc gcctgcttgg cagatccgcc tgcggagtca ccttccacaa ggaacagttc
     1801 ggtacggtta agatcctgcg cggtacagtc agccagtttg ccaggcagtg ctgggccgct
     1861 ggtcagcttt ttgcgcacca cttttttagc cgcacgcata cggcgctggg cgctggaaat
     1921 cgccatctcc gccagcagct ccgccgcctg aacgttctga ttcagccaca ggataaaggc
     1981 atctttcacc acgcctgaaa cgaatgccgc gcattgacgc gaagagagac gctctttggt
     2041 ctgcccggca aactgcggat cctgcatttt caccgacaga acataggcgc agcgatccca
     2101 gatatcttcc gccgacagct tcacgccgcg cggcagaatg ttgcggtatt cacagaactc
     2161 acgcatcgcg tccaacaggc cctgacgcag accattaaca tgggtaccgc cctgcatcgt
     2221 tgggataagg ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag
     2281 cgcccagtcc acagcttcag tatcgccagc gaaattaccg ataaacggtt tttctggcag
     2341 cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca
     2401 gcgctgttcg gtgttgttga tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac
     2461 tgctttggcc ttcagcacat gcgtcaggcg tgaaacagaa aaacgcgggc tgtcaaagaa
     2521 ggtttcatcc ggccagaagt gcacgctggt accggtattg cgtttaccgc aagtgccgac
     2581 aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc
     2641 gcggcgcacg ttaacttcta cgcgcttcga cagggcgtta accaccgaaa tccccacgcc
     2701 atgcaggccg ccagagaact ggtaattttt gttagagaat ttaccgcctg catgcagacg
     2761 gcaaagaatc agttcaaccg ccggtacacc ctcttccggg tgaatatcca ccggcatccc
     2821 gcgcccatcg tcaataactt ctaacgactg gtcagcatgt aagattacat ccacgcgttt
     2881 tgcgtgaccc gccagtgctt catcgacact gttatcaata acttcttgcc ccaaatggtt
     2941 agggcgagtg gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag
     3001 tacctcaatg gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg
     3061 atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc
     3121 ccgtgaatgc gtggttgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca
     3181 ccgcctggcg gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg
     3241 gcgcttccag cgggtcaatc tgcatgactt tgagatcgta aatatggcgt gactctagca
     3301 catattgctg cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcagttcaa
     3361 acgggcgtac cgccgggttt accaccactg cgggcagcat aaaacattgc gacaaccagg
     3421 tggcgtaata tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca
     3481 ggacaatgga ttccaacagc tctgccgcgt cggaaggata cggcggcaac tgcggaatga
     3541 tcatctcaac gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag
     3601 agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga
     3661 agcggtatca ggttggaaac gtttgtccgc caggcgatgc acctcggtgg tcagcgtgcc
     3721 atcagcatgt aactcgagag tacgccagcc gggcgcgatg gtgtccagcg taaagttgga
     3781 acagtgcggc ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca
     3841 gtcgagatcc agctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt
     3901 cgccagcacg gtatccagtt cgcccgcgtt acgcagactg tgttgatcga gccaactaca
     3961 acccgcaggt agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc
     4021 cagtttacgt tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa
     4081 cacctggcta tccagcaaca ggatttgcca ttgctcacca ataaacacgc gcttcgccgg
     4141 ggagataccc gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg
     4201 cagccagacg cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc
     4261 agaggattga tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctgatg
     4321 tggacgaatc gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaacagggc
     4381 ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt aaaatcctga ctctggcctc
     4441 accagccaga ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta
     4501 gtaaaccgga atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa
     4561 ctggttaatt tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg
     4621 cgctttgaag cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata
     4681 cagacgcacc gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat
     4741 tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc
     4801 cacctgatag cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg
     4861 tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat
     4921 ttatgcccac tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac
     4981 cgacgctgcg ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac
     5041 cacatgaacg cgaatatctt cgttttcatc agccagacca tgaataccgc ttgcggtcgt
     5101 ggcgtccact tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag
     5161 gaaacttaac accggtttgg tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg
     5221 acgggcaaca tcttccacac tttcaccctc ttcaatcatc ccggcaacca tctccagtag
     5281 ccagggggtt tcgctggtgt cgtacgcggc aatccgaatc tgctcaatca gcacaacttc
     5341 atcacgcact gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg
     5401 ccgtacctca tgactcattt gcccgttgaa tagacgatga cgaaatctat aaagatctaa
     5461 tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa
     5521 tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc ctggcactaa tagtgaatta
     5581 aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt catgtgttaa attgaggcac
     5641 attaacgccc tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg
     5701 caataatttt acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag
     5761 aaattgctcc ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc
     5821 gagaacctga tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct
     5881 gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca
     5941 cagctaggtt taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc
     6001 aactctaacg cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa
     6061 tggcgtgcgt taacgctgca ggaaaaagca gcagggattc aggacgtcac atatcagacc
     6121 gatcagcaaa ccttgatcct caacaccgcg accgcttatt ttaacgtgtt gaatgctatt
     6181 gacgttcttt cctatacaca ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc
     6241 acccaacgtt ttaacgtggg cctggtagcg ataaccgacg tgcagaacgc ccgcgcgcag
     6301 tacgataccg tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag
     6361 cagctgcgcc agatcaccgg taactactat ccagaactgg cagcgctgaa tgtcgaaaac
     6421 tttaaaaccg acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac
     6481 ctgtcgctgt tacaggcacg cttgagccag gacctggcgc gcgagcaaat tcgccaggcg
     6541 caggatggtc acttaccgac gctggattta acggcttcta ccgggatttc tgacacctct
     6601 tatagcggtt cgaaaactcg tggtgccgct ggtacccagt atgacgacag caatatgggc
     6661 cagaacaaag tgggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg
     6721 caggtgaaac aggcccagta caactttgtt ggtgccagcg agcaactgga aagcgcgcat
     6781 cgtagcgtcg tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcatc tatcagtagc
     6841 attaacgcct acaaacaagc cgtagtttcc gctcaaagct cattagacgc gatggaagcg
     6901 ggctactcgg tcggtacgcg taccattgtt gatgtgttgg atgcaaccac cacgctgtac
     6961 aacgctaagc aagagctggc aaatgcgcgt tataactacc tgattaatca gctgaatatt
     7021 aagtcagccc tgggtacgtt gaacgagcag gatctgctgg cactgaacaa tgcgctgagc
     7081 aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt
     7141 gctgatggtt atgcgcctga tagcccggca cccatcgttc agcaaacatc cgcacgcact
     7201 accaccagta acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc
     7261 gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc
     7321 ttcaatttcg accagccatc ctctattctg atgggtattt accactggtc ccggaagaca
     7381 aaaatgaaac ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcgcgc
     7441 catctgacac cagtcgctct cgcggttgcc actgttttta tgctggctgg ctgtgaaaag
     7501 agtgatgaaa cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc
     7561 aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg
     7621 ccgaaatacg ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgccagcag
     7681 gcaccagccc aggctggcat ggcaccagaa aaccaggcgc aggcccagca atccagcggg
     7741 agtttctgga tgccgctgat ggccggttac atgatggggc gtctgatggg cggcggcgcg
     7801 ggatttgcac agcagccgct gttctcctcg aaaaacccag ccagcccggc ttacggtaaa
     7861 tataccgacg cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta
     7921 ccgaagacag ccatggcacc aaaaccggcg accaccacta ccgttacccg tggcggtttt
     7981 ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg caactggcac ctcttctcgt
     8041 tcaatgggtg gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg
     8101 agaaagccca cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg
     8161 aagatgctta ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg
     8221 aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga
     8281 ccaaattccg cattccaaaa cacacctgga gttttgtgcg ccagtcatgg ctgacgcacc
     8341 agccatcgct ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa cctaaacttc
     8401 tggaaaataa cgccgatacg ccaacgtcac tatacgaggc ggcgttcttt cagtggatct
     8461 ggctggaaga tcagcttaac gccggtaact tgccggaggg cagcgaccag tttaacagtc
     8521 tgcaagaaaa actgatcgat cgcttcgttg agctgcgtga acagtatggc ttccagttgc
     8581 tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg
     8641 actgcgcaac ggaagctgaa attgctactg agttcctcta catcgatgat atcgggttag
     8701 gtgaaaaagg tcagttcacg gatttacagg atcaggtaat ttccaacctg ttcaagctgt
     8761 atccgtggga atttatgttg cgtgagatgt tttccaccaa gctggaggat gcaggcgtac
     8821 gctggctgga accggcgtgg aagagcatta tctccaacaa ggcacttcta ccgctactgt
     8881 gggagatgtt cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc
     8941 cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga
     9001 tcattgaaaa cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga
     9061 ttgttcagca attccacccg ttaccgaaat tcggcgacag ctatatgctg attggtagct
     9121 ggctggtgaa cgatcaaccc gccggaattg gcattcgtga agaccgtgca ttgatcaccc
     9181 aggatatgtc tcggttttat ccacatattt ttgttgagta atcaatgatg ccggatggca
     9241 ttcgccatcc ggtaattgtt aacctatctg caccgacagc atactcaggc tgcccatttc
     9301 tataccatcg accggaatgg taattggctc ctgcccatcc cacgcaccta agacatacaa
     9361 caacggcaga tagtgctccg gcgttgggtt cgataacgcg ccaccttcat ggtcgaggta
     9421 attcaccaga ggatgctgct ctaccgcccc ttgccacgtc agattcgctt tcacatactc
     9481 attaaacgac atcgcccaag gatatggtga actatcaccg tgccacttca ctgtacgcag
     9541 gttatgcacc acgttgccac tggcgaccaa cattattcct tcatctcgca gcgctgccag
     9601 tttgcgcccc atttcgaaat gccaggcggc aggtttgcta ctgtcgatac tcaactgcac
     9661 catcgggata tcggcgtcag gatacatctt aatcagcacg ccccaggaac cgtggtcaaa
     9721 gccccaggct tctttatcca gcgtcaccgg gaccggcgct aacagctcaa ccagatgctg
     9781 tgccagcaca ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc
     9841 accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa
     9901 ccagtgagcc gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg
     9961 ccagctgcgg gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa
    10021 caatgctggc atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat
    10081 atcctcacaa tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc
    10141 agttattttg acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc
    10201 tctcattctg accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct
    10261 cgggcaaaaa ccctcgaacc gcttactggc gttttcgcta ggatttgcgg cggggatcat
    10321 gttgctcatc tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc
    10381 tgtgttgggt tatggaatgt ttatcttcgg tctgcttggc tattttggcc tggaccgcat
    10441 gttgccacat gcgcatccgc aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc
    10501 gatcaagcgc acagccattc tgctcactct cggcatcagt ctgcataact tcccggaagg
    10561 gattgccacc tttgtcacgg cgagcagcaa cctggagctg ggatttggca tcgcactggc
    10621 cgtcgctttg cacaatatcc ctgaaggtct ggcagtggca ggcccggttt atgcggcaac
    10681 ggggtctaaa cgcaccgcga ttctgtgggc ggggatttct ggactagcag aaattcttgg
    10741 tggtgtgctg gcgtggttaa tcctcggtag catgatttcc ccggtggtca tggcggcaat
    10801 catggcggcg gttgcaggaa ttatggtggc gctctcggtt gatgaattaa tgccgctcgc
    10861 caaagagatt gaccctaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat
    10921 gggattcagt ttagtgctgc tacaaacggc gggaattggt taaaaacaac atcagaagtt
    10981 gtccagcaca tggacaactt ctgccagctt cacgaaagcc ttattctacc ttttcggctg
    11041 tctcttctct cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct
    11101 ggcgaaacgc ccgacaataa cgtttcaaat gcaggaaata acccaccaca accagagcaa
    11161 tcaatacaat ccagtaccag gcgataaaca tccttttacc cttaatattt aacaatacta
    11221 aacatttagc gcataaattt cacatatcct ttttcggata tattcatatg gtcgcataat
    11281 aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt
    11341 ttctcataga aagagaagcg gaattaacat cttgcatagc ggggcaaaat aaaaaccgaa
    11401 gccagctaca acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc
    11461 tttacgctca tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt
    11521 gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt
    11581 cagctcacac agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc
    11641 ttcagtatga ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg
    11701 gcccgggcga ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt
    11761 aatacgatca gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt
    11821 aaaaccggtg ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt
    11881 acggcgatct tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc
    11941 catctgctca acggtcatgg tttctgccgg gaagatcaaa tcgccttcgt tttcacggtc
    12001 ttcatcatca agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac
    12061 acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca
    12121 ctaaaattat ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata
    12181 acgtgagcgg gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc
    12241 ggccccggaa ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc
    12301 tttcaactga gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga
    12361 ttcactataa cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat
    12421 gacttaccct tatcgcacta caatggcact caacacctat cagtacaggg aaactacaat
    12481 gattgacccg aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg
    12541 aatcagggag ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct
    12601 gacgcgcctc gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg
    12661 gactcgtgag aaactggcgc tgcttgaaca aagaatgagc gaactggaaa atcgcagtac
    12721 tgagattaaa aagcagccag atccagagac tctcccccca acgctgtaaa catttgtctt
    12781 taatgcactg taaattatga attaatcctc taatttacag tgctttctat taccttatcg
    12841 cttaattcca ttcagaatat c
//
LOCUS       DAZFRN010000137        12843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFRN010000137 DAZFRN010000000
VERSION     DAZFRN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.139"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(106..825)
                     /gene="dcuR"
                     /locus_tag="ACHKUB_003849"
     CDS             complement(106..825)
                     /gene="dcuR"
                     /locus_tag="ACHKUB_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO6656810.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(822..2453)
                     /locus_tag="ACHKUB_003850"
     CDS             complement(822..2453)
                     /locus_tag="ACHKUB_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO6656811.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2634..2864
                     /gene="yjdI"
                     /locus_tag="ACHKUB_003851"
     CDS             2634..2864
                     /gene="yjdI"
                     /locus_tag="ACHKUB_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO6656812.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2876..3148
                     /locus_tag="ACHKUB_003852"
     CDS             2876..3148
                     /locus_tag="ACHKUB_003852"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6656813.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3375..3671
                     /gene="ghoS"
                     /locus_tag="ACHKUB_003853"
     CDS             3375..3671
                     /gene="ghoS"
                     /locus_tag="ACHKUB_003853"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6656814.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3699..3872
                     /gene="ghoT"
                     /locus_tag="ACHKUB_003854"
     CDS             3699..3872
                     /gene="ghoT"
                     /locus_tag="ACHKUB_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO6656815.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(3991..5508)
                     /gene="lysS"
                     /locus_tag="ACHKUB_003855"
     CDS             complement(3991..5508)
                     /gene="lysS"
                     /locus_tag="ACHKUB_003855"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6656816.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5745..7202)
                     /gene="dtpC"
                     /locus_tag="ACHKUB_003856"
     CDS             complement(5745..7202)
                     /gene="dtpC"
                     /locus_tag="ACHKUB_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO6656817.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7261..9408)
                     /gene="cadA"
                     /locus_tag="ACHKUB_003857"
     CDS             complement(7261..9408)
                     /gene="cadA"
                     /locus_tag="ACHKUB_003857"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO6656818.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9488..10822)
                     /gene="cadB"
                     /locus_tag="ACHKUB_003858"
     CDS             complement(9488..10822)
                     /gene="cadB"
                     /locus_tag="ACHKUB_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO6656819.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11187..12725)
                     /gene="cadC"
                     /locus_tag="ACHKUB_003859"
     CDS             complement(11187..12725)
                     /gene="cadC"
                     /locus_tag="ACHKUB_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO6656820.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGNLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         3531 a         2969 c         2985 g         3358 t
ORIGIN      
        1 attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt
       61 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
      121 tttcagcagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac
      181 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtattttcg
      241 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
      301 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
      361 caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc
      421 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
      481 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag
      541 cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
      601 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
      661 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
      721 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
      781 ctccgcgacc attgcgtcgt catcgataat taatacattg atcatctgtt cgacctctcc
      841 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
      901 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
      961 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
     1021 ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
     1081 atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
     1141 gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagcag gctttcactg
     1201 tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca
     1261 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctg atagttattg
     1321 gctgttttga gaatgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
     1381 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg
     1441 accagaccgt cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa
     1501 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
     1561 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
     1621 tctaccacct gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta
     1681 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc
     1741 acggcaacga cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg
     1801 gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag
     1861 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
     1921 tcattgattt gttgggtcac gcggcttaac tcaaggccga tcgccaccac gccaatttgt
     1981 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga
     2041 ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc
     2101 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc ggtaacgaca
     2161 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc
     2221 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact
     2281 gccagtgcct tgttggccag cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc
     2341 agatgcacca ccaatagcac cgagaacagt accgcgctga ccattaagat cactgtggta
     2401 ctcaatttca tcggacgttt gcgtaacatg cggtagggca atgaatgtct catcagcttc
     2461 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa
     2521 gaatgattgg ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa
     2581 gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc
     2641 aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
     2701 acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
     2761 tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga
     2821 ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
     2881 aatacgcgaa ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga
     2941 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
     3001 aagcctgaaa gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
     3061 tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac
     3121 gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg
     3181 ctctccgcgg agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc
     3241 agagagcgcg tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc
     3301 ctatatgaga atcatcaatc ggggttaata atttttgcgt ccccagagca ttcaatattg
     3361 ataggagtca tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt
     3421 atccatcatc ttattcctca gtgttcttaa ggttaagatc attgatgtat gatatgaatt
     3481 tctcttctat cgtggctgat gaatatggga taccacgaca attgaatgaa aattctttcg
     3541 caataacgac atcgttagcc gcaagtgaaa tcgaagattt aatcaggctt aaatgcttag
     3601 acttaccgga tattgatttt gacctcaaca ttatgacagt tgatgactat ttccgtcagt
     3661 tttacaagta gccaggaagg ataagaagaa cataaaaaat ggcactattc tctaaaatat
     3721 taatttttta tgtgattggt gtgaacatat cctttgtcat tatctggttt atctcacatg
     3781 agaaaacaca tattcgttta cttagtgcat tcctggtcgg aataacctgg ccaatgagtc
     3841 tgcctgtggc attacttttt tctctctttt aggagctgta gttgagtgga cccgctggtt
     3901 atcgtgaaac gaagcgtctc tcctcaaaac tggatttgcg gaacgacgaa cgaagcagtc
     3961 gcctgactgc ttcgttcatt aaagtgaaat ttatttctgt gggcgcatcg ccgggaagag
     4021 aataacgtcg cgaatagtat gactgtttgt aaacagcata atcattcggt cgataccaat
     4081 acccagacca gcggttggcg gcagaccata ttccagcgca gtcacgtaat cttcgtcata
     4141 gaacatggct tcgtcgtcac ctgcagcttt agcgttaacc tgttcctgga aacgttcagc
     4201 ctgatcttct gcgtcgttta attcgctaaa accattaccg atttcacgac caccgatgaa
     4261 gaattcaaaa cggtcggtga tttccgggtt aacatcatta cggcgcgcca gcggtgacac
     4321 ttctgccgga tattccgtaa taaaggttgg ctgaatcaga tgtgcttctg ccacttcatc
     4381 aaagatctcc gtgacaatac gtcccagccc ccagcttttc tctaccgtaa ttccgataga
     4441 ttcagctaat gctttagcag catcaaaatt atccaggtcg gccatatcgg tttctggacg
     4501 atattttttg attgcttcgc gcatggtgag tttttcaaac ggtttgccga aatcaaacac
     4561 atgctcgcca taagtgactt tagtggtacc cagaacctct tgtgccaggg tgcggaacag
     4621 ggactctgtc agttcaatca aatcgtggta atccgcatac gccatgtaga gttccatcat
     4681 tgtgaactca ggattatggc gaacagaaat accttcatta cggaagttac ggttgatttc
     4741 gaatacccgt tcaaaaccgc ctacaaccag acgtttcaga tacagctccg gcgcgatacg
     4801 caggtacata tctaaatcca gagcgttatg atgggtaata aacgggcgag cagatgcccc
     4861 acctggaatt acctgcatca tcggggtttc tacttccata aagccgcgcg cgaccatgaa
     4921 ctggcggata gcggccagaa tttttgaacg gacaacaaac gtttgacggg atttatcgtt
     4981 agcgatgagg tccagataac gttgacgata acggacttcc tgatcctgca gaccatggaa
     5041 tttatctggt aaaggacgta gtgcttttgt cagcaggcgc aactcagtac agtgaatgga
     5101 aagctcaccg gtttgcgtct taaacagcgt accgcgggca ccgataatgt cacccagatc
     5161 ccatttttta aactgatcgt tataaacacc ttctggcagg ctatctcttg caacgtacag
     5221 ttgaatacgg ccaccgacat cctgcaacgt tacaaaggag gctttcccca tgatacgacg
     5281 ggtcatcatt cggccagcaa ccgagacttc aatgtttaag gattccagtt cctggttatt
     5341 cttcgcatca aactcttcgt gcaactggtc agaggtatgg tcgcggcgaa aatcattggg
     5401 aaacgccaca ccttgctgac gtagtgctgc cagtttttcg cggcgatttc tcagttcatc
     5461 gttaaaatca atagcctcat tggctccccg tgtttcttgt tcagacattt tggttcctct
     5521 aaatccagct ttcaatttag cgtagataaa gagacagatc ggtacactgt attatctgcc
     5581 tcgactatta ataactcagt ttatcttatc gaaactaacg cttacctcaa gttgatatca
     5641 ctaataaata accatcaaaa tggtttaaat gacttattaa tctcgattta ctagtgtgat
     5701 taactgaaag ttaatttatt cataaccaat tgtttttacg acagttaatc gttgctctcc
     5761 tgtatcatat tcgtcggcgt gctaaacaga aaacgggtag caaaggccaa tacgacgatg
     5821 atcgcgacac aagccaacgt ccactctccc atctgagaaa agaatcgctg gtaagctgca
     5881 attgccatac cgctaatttg cgactctgtc gtctgctgtg ccacgacgcc tgccagccag
     5941 ttggcgaccg cgcctgttgc cagcatataa ataccggtta atacgccaga cattttcaga
     6001 cgcgtgattt gcgcaatcgc caccgggtca ataaagagtt cggcaaagcc cattagcgcc
     6061 agcccggata tcatcacggc cattgacgct tgaccgccag ccgctgcatg tcgggcatca
     6121 aatgccaaca acataaagcc acaagccatc agcagtaagc caaaggcaaa cttcagccag
     6181 acgcgcaatg ttgagttgcc gcggctttct ggcgacgcca gccaggccag tacaacccca
     6241 gcgagcatca ctgcaattgc attcaccgac tggaatagtg ctgtaggtac ttcaatattg
     6301 aatgcctgac gattcacaaa gcgatcgata aacaagctga tggtactgcc gccctgttgt
     6361 gccagtaccc agaacaacgt cccgacaaac atcaacaata caatttgcca aagagcacgg
     6421 cggtgttcgg ggaatttgat catcatgcgg gcaatgattt gtgcggcaat gaggcaaacg
     6481 atcgccagca aatatccgga ccagtcgttc tccagcagca gggtaaaaaa tactggggct
     6541 aaacagagca tcaccactaa ccagctccat accggtaagg caaatttgac gctagtgagc
     6601 gcttttttat ccatactacg cgtggactgg aaatgacgat gcccgcttaa gaaaatcaac
     6661 aaaccgataa acatgccgcc acccgcaagg gcaaagccaa catgccatcc ataccactga
     6721 gcagccaggc cgcaggcgat tggggcggcg atagaaccga tattgcccgc agcatacagc
     6781 agcgaaaaac cgccatcacg tcgatgatcg ttctcgtcgt aaagctcgcc aagcaaacag
     6841 ctgatgtttg atttgaataa accgtagcca caaataatga ttgccagcgc cagatacagg
     6901 ctaaaggttg aatttgtatc aatgcccagc accacatggc caagggtcat taacagcgcg
     6961 ccggcaatca ctgcagtgcg gttgccgagc aggcggtcgg caagccagcc gccgagaata
     7021 ggggtaacgt aaaccagaga agcatatgcg ctgaacaggc tgatggcatg gttatcatca
     7081 aaaccaagct gatgggtgag atagagaatg agtaaggcac gcatgccgta aaaactgaag
     7141 tactcccaga tttggatcgc cacgatatag tatatcgcgc gcggctgtga gggtgttttc
     7201 atgtgttctc cttatgagca aaaaagggaa gtggcaagcc acttcccttg tacgagctaa
     7261 ttattttttg ctttcttctt tcaatacctt aacggtatag cggccatcag cctgacggta
     7321 tgcaccgtga atatcggttt caaagcccgg atagtgagcg ccgatttcac acagcatctg
     7381 caggaactcc agaaccggac ggctttcttc ggtgatcatt tcacccggca ttaccagagg
     7441 aactcccggc ggatatggaa ggatcatatt ggcgttaata cgacctacca tttcgtcgag
     7501 gtaaacttct tcggtcatac cgtgcagctc tttctggaac gcagcatacg gagtcattac
     7561 catcgtcggc agcacttcaa atgcgcgata catcagatcc ggcagattgt ggtgaacaat
     7621 cagtttgtgg atattctgag ccagttcctg aatacgcatg ttttcataga attcaggatc
     7681 ttcacgatac agagacggca gcatgttttt cacacgcagg ttcaggtcga acgcacgttt
     7741 gaagtcagtc agagcacgca gcaggctcag tgctttggtc ttatcgatac cgatgctgaa
     7801 caggaacagc aggttatacg gaccggtttt ctcaacaacg atgccatgtt cgtcgaggta
     7861 tttcgccacg atgctggccg gaataccaaa gtcgctcatg gtgccgtctt tttccatccc
     7921 cggagtcagc agggtgactt tgatcgggtc aagatacatg tgctcgttat cgatgttttt
     7981 gaagccgtgc caggtgctgt cagaacgcag cggccagcat tcagtcgtat cgatatgatc
     8041 cggctgccat acatcaaaga accagccatc agattccgtt ctcagacgtt tgatctcttt
     8101 acggaatttg atcgcacgtt caatggaacc gttgatcaga cgtttacctg cattgccttt
     8161 catcatcgcc gcagcggttt cagtggacgc cacgataccg tagtgcggag aagtggtggt
     8221 gtgcatcatg taggcttcgt taaaggtttc ttcgtttacg tcacctttaa cgtggatcat
     8281 ggaagcctga gagaacgccg ccagcagttt gtgagtggac tgggtttcgt aaatcacttt
     8341 cccttctaca cggccaccgc tcataccgca tttaccttcg taaatcggtg agaagttggt
     8401 gtaaggcacc cacgcggagt caaagtggat ggatttcaca tccagtgttt tcttgatgaa
     8461 gtcggtgttg tacagcagac catcataggt agagttggta attacagcat gtaccggcca
     8521 ggttgcgttt ggtgtttctt tcacgcgctt agcaatggta gcgtgctgga attcactctg
     8581 tgggatacca ccaagaatac cgtaagcgtt acgggtcggg cggaaataga ttggcgtaac
     8641 atcgctcatc atcatcaggt gggtcagcga tttgtggcag ttacggtcaa tcagaatggt
     8701 gctgcctgcc ggagcagagt acatgccaac aattttgttc gcagtggaag taccgttggt
     8761 caccatgtag ctgcggtctg cgttaaagac gcgagcgata tactgttctg cttctttgtg
     8821 tggaccactg tgatccagca gagaacccag ttcagatact gaaatggaaa tatcagattt
     8881 catggtattc ggaccaaaga aatcatagaa caggctacct accgggcttt tctggaatgc
     8941 agtaccgccc atgtgaccag gagtacagaa agtatattta ccttcacgaa catatttaaa
     9001 cagtgcttta gtcagcggag gcagaatagt gttgatatat tcgtcagtgg tctgcttgat
     9061 cttattagca atatcttcag cagcacccag cgcatattca aagaagctaa tctgtaaacg
     9121 caggtcattc aggcttacat cgagagtgga atacgtatta gcgaacgcgt acaacggcag
     9181 gttctcgttc attttgctaa tttcttcgca cagctcgaga ttatatttat cccagtcaaa
     9241 aataacgccg cacagacgcg cattgttttc gatcagtttt aataagtcgt cacggtcgtt
     9301 cgggtaaaca atctggaagt tcagacgttc aagcgcgcga tgaagttcac ggatgggttc
     9361 ttctttaaaa taaaccccca tgtgattcaa tattgcaata acgttcatag tcatatctcc
     9421 aggtaaaaaa ggcccctccc aacacatggg acaaaatgaa aggaggagcc tcggaaaata
     9481 cttttaatta atgtgcgtta gacgcggtgt ggttatccat tgagtggctc tggcgctcgt
     9541 gcattttgcg agcgtagaac atcaggataa tcaggctgac gatgaaggta cctgccagct
     9601 cgaaggagct tgcgcccatc agcgcgatga agcagaacac acaacccagt acagagcaga
     9661 tcaggctgac aaagttgcgg atgttaacgc cttcaaaacg aatcaggtca acgcaagagt
     9721 agaagtacgg cagcatagtc agcagtactg cgataccggt cagttcaccg aacaggtcag
     9781 atgctttacc accggcagag ttcatcagag tgatgaggat catcagggca gtcattttca
     9841 ctgcagccag cagcagacct tttttcggaa taccgttgct gtcgacttca ccataaactt
     9901 tcgggaagtt accgtcgtta gcggcacgta cacctgcctg gcctaccaac atcatccagg
     9961 agcccagaga agtcaggcac gcaaaggcgg tgaatgcaga aaccagcggc gcagcccagt
    10021 taccgaggat ggttgaagca ctgattgcaa acggagcacc ggaagctgcc attacagaag
    10081 atggatacat accggaaagc acctgagtcg cagcgatgta aacaatacct gctaaaccag
    10141 tacccagcat ggttgccagc ggaacggtac gtttcgggtt tttaaccata ccagtactta
    10201 ctgctgcgga ttcaacaccc acgaaggccc acaggcagag cagaatactt ttaatgatcg
    10261 catgaccatc agtggtatcc gcagtattcc agttagctgc ataagttgcc gcatcaaacc
    10321 aatgccagcc aacaatagcg gtcattacta ccggaataag aaccagcacc agaccaatag
    10381 tggttaaacg gcttacccaa gtaccgccga gcatatttac aaaggtaaat acccagacga
    10441 tagcaataca ggcgataccc gccggaacag gatcatttaa tactgggaag aaggtggaaa
    10501 gataagatac cgcggtaata ccaatcgcca ggttaccaat ccagttagca tggtaataaa
    10561 gaacacctgt ctgaaaacca aatgcagggg aaatttctcc ggcataagca attgggccac
    10621 cttgttgcgg gttttttgtt gccagtcggg catatacata cgccagcgac attgcaccaa
    10681 taatagagat aatccaaccc cagatagcaa taccaccgat acttgctagg ttcgcaggta
    10741 ataatgcaat accgctcccc atcatattac cggcaacaac accggtacag gcaaatagcc
    10801 cgatcttctt ggcagaactc atgctcttct cctaatttca tttttgaatt tggagtccgg
    10861 gtcatgatgt ataactattt cctgaccaga ccaaactggc gctaagaata ctcacgaaaa
    10921 aaggattaat cctaaagatt aggtgaaata acacaaaagt ttctgtaagt gagaacttga
    10981 ggttttttat taacacatca ggatcgcaag ttgatatcat gaaaagataa acattcaatg
    11041 tttacaatgg attgcatgac attctctggt taaatttatg taataaaaat tatgcggcaa
    11101 ataaattgcc gcaacatatt ataccaacgg gaacatacaa aaactcaaca acaaatattc
    11161 cagagcataa atcaacccgg agttacttat tctgaagcga gaaatttgtc gagataaggt
    11221 acaacataag gaacagaagt ctggaatata ccattttcaa tccagtaaag ggtgtttgcc
    11281 cctgggcgta aattaaaggc ggtgagatat gcatcagccg cttcccggtt catccccttc
    11341 atttcataaa ccttgccaag caacacataa tttagccagg acatttcaag atcaatgcca
    11401 gtatttatcg cctggtaaga ctcatctgtt ttacctttta ccagagcact gaccgctttt
    11461 atttgatata taatggacag gttattcaat tccggcagtg taacaatgtt gtctatttct
    11521 gtgttcagtg ctgctaattg tttttcatct aaaggatgtt gagaatggcg cacgatatca
    11581 actaatgctt tttctgctct cgcgtaggta aattctgggg atgattgaac aatctcacct
    11641 aataattcac tggcacgatt caatgattta tcatcgccat gcagtaaata atcatgtgcc
    11701 tgataaaaat tagttaataa cgcaccacga tgaggcaata tttgctggag cgtctcctgc
    11761 attcgttgtg gccacggttg gtttaacgct tttgataaac tctccagtaa atcattttga
    11821 atcgccagct gattaccgtt agtgatgaca taacgtttat ccagcatggt tgaaccatct
    11881 gcattgtcta ccaattttat cgacataaag cattgttgag cacggtattg gcgctgatta
    11941 acaaacgcaa tagataatgt tttaccggaa ctgctcggtt catcaatgtt gtagttgatt
    12001 ttgtcatgca ccataaaggt ggagaaggtg ttaagtgatg tcgccaccaa attacccacg
    12061 cctatcgcgt aagagagctg atacggggaa ctccagctgt tacaactttt atttaccata
    12121 ttaatgtcaa tatcgcgtgg attgagcaaa atacgcgatt tgctcatagg aagacgtgta
    12181 tcaagagttg aaaacgctac cagtgctaca cagataccta acgacaacag gaaaaaaaac
    12241 catacccaaa aggtagtgaa tcgtttgctt ttaactgggg attgttcagg tggcgttgcg
    12301 gtgttttgaa tgttaagact gtgggaggga gaatctgtgg caggaaccgc ctctggtata
    12361 gggggaggcg aagatagcat tatttcctct ccctcttctt cgctgtacca gataaccggc
    12421 accattaatt tatagccgcg ctttggtaca gtagcgatat agacaggact atcttcatca
    12481 ttatctttta atgacttacg tagttctgag atactctgcg tcacaacatg attggtgaca
    12541 atacttctct tccagacatt atcgataagt tcatccctgc taagtacttc gccactgtgt
    12601 tgagcaaaga aaaccagaag atcgattaat ctcggctcaa gggtaagttg acgcccattg
    12661 cggctaattt ggtttatgga cggagtaaca agccattcgc caacgcgaac tacaggttgt
    12721 tgcataatag aaactcattc gaaaagggaa tgatgcaatg ataattgcca caacctattt
    12781 ttaccatcta tagatgggtt tatttacata ttattggtga atgtaagacg ttatttttac
    12841 cag
//
LOCUS       DAZFRN010000138        12666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFRN010000138 DAZFRN010000000
VERSION     DAZFRN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.140"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..8896)
                     /locus_tag="ACHKUB_003860"
     CDS             complement(<1..8896)
                     /locus_tag="ACHKUB_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO6656821.1"
                     /translation="MNRNCYRIIFNKARGMLMVVADIARSGRAGASPSSRTGYPRRQR
                     ICRVTPLAFSLWLASGMVHSVSAAGIVADHGAPGHQQPTIMQTASGIPQVNIQTPSAG
                     GVSHNTYSQFDVGNQGVILNNAHNNVQTQLGGMVAGNPWLAKGEARIILNEVNSRNPS
                     QLNGFVEVAGKKAQVVIANPAGISCDGCGFINANRATLTTGQPQMKNGSLTGFRVEGG
                     EIQVTGKGMDASRTDYTDIIARSVKINAGVWAQDLKVTTGRNNVDITHGQTEKKAADA
                     SSQPQVALDVSSLGGMYAGKIRLVGTETGVGVRNAGHIGAQAGAVTLTADGRIENSGS
                     ISAKTDVHLATTRELHNSGSVYAGQDTQIQSDGAFTHTGSVASRRNTRIQTTRLTGSE
                     RSLLAAGVKDDGRLAEAGNLTVSTTGVLAAHGQVLSGGNMQLKGQGLDLSNSRIQGQH
                     TGLDAGSGNLSTQNAQLSAGTLSARTAGQFSNNDGTINADTLQISAQSLSNRKGSLIQ
                     TGTGDFSLSLPGSVDNREGLLAANGAVRLDALSLDNRRGKVQAAQSGSLQVKTTGAVD
                     NQQGRLLASSDILINTQALNNDNGLISAAAGTGRIKTQQSVSNTEGRMESAGRLDISA
                     GSLNNHQGTVVSDGLSVTLDGALDNTSGRLLSQKTLSVSGSELVSDDGLIQSGSDMTL
                     DVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNNQQGFIAGQKDVTLNTGTLDNRQGAL
                     GSQASLQVSSGTLMNQKGALKAGTDMLLSGDDVSNQEGTLAAGGDLNAHLNVLENQQG
                     TVVSNGNSRLDVTRSDNQGGRLVAQQSLTLSSTDIINDAGGLIQSGASLNLRTDTLSN
                     RNSGDRGGVISQGPVTLNAGTLDSTAGVLLSGDALSLTAGVVNNTSGQIVASGQIVAN
                     GLPGRNSLALNNQSGLIQGKGISINTAGRTLDNRRGTLNSLQELTVSTGAMDNRGGTM
                     GAKTTADLSTTSLDNREGGRLVSEGEIRLHTRGLQNSHGQIQSVGDMLLDSVRGVVDN
                     VSGLIRSGSAITLNALQFINRHTQNTGQGLEAQTIHITTQDLDNQEGSILADRALTVM
                     ADRTLSNNDGVLSSGATLFVSGRQLAFSNRDGVVKAGQSVSVTAGQLGGDGKLLSPGD
                     ITLKSNTAFSNSGQTIANGNLTLSVNGDVTNTGSLLAGSRLELNSIRLENTEKGEISA
                     GQTWLNVTDTLLNRGLIDGKYTRLQANTLTNSGTGRIYGDAVGVSAATFNNLEENGVA
                     ATLAGRERVDLGVQTLNNRTHSLIYSAGDMHIGGTLDANGSATGKAGVLNNHSATVEA
                     TGSLALSAGQINNVNDHFTTERVVVSTEKVTEYQLSGSDKRWSAGEPGVYVDNDSSNS
                     LKKLHTPEGARDKFTQYDYTRTVEETRVKDSDPGKILSGAGMTVVADKLLNDKSLVVA
                     GGLLSIPSGSVENVSVSGERHVTDSGTSTYYYRIRKKGKDKQGEKTSQYTPPTVIQTI
                     TLKPGELTSHGQVQGSHVTLSPLKPQGTDVQTGQAGNVDATVAATDRIPLRPVVSAGE
                     PVILLPGQQFEVSTPQGSIHVAGPDTRLPDSSLFKTNPAVNVPYLVETDPRFTNQKTW
                     LGSDYMQKAFSQNGDNMLKRLGDGFYEQRMIREQVVALTGQRFLDGYGSDEEQFKALM
                     DAGIAFGKQYNLKPGVALTAEQMALLTGDIVWLVNTTVTLPDGSTQTVQVPQVYARVK
                     PGDVNSAGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLSAENIHNQAGSIQGANVSLT
                     ARTDINSTGGLLQATDSLLAMAGRDISLTTTTRTAQRDAGQNHFERTSIDSVAGVYVQ
                     NDQGRLVLQAGRDMNLTAATVVNQGKDSLTQLSAGRDMTLSTVTTSAQDNIIWDKNNR
                     LSQGITQSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKGLALMAGHDVTLTGAQNTRS
                     LDEYHKVTGSSGMLSKTTTTTHDVTDRRTMTGSELNGDTVSISAGHNLNVTGSSVAGD
                     NRVSLAAGNDLSIGTLTESNRETHLKQEKKSGLMSSGGVGFSVGSQSLKVTDTATDST
                     QKGSTVGSVHGDVSLQAGNRLSANSADLIAGRDMALSGKEVSISAVTDRHVQTHTVEQ
                     KTSGLTLALSGTVGSALNTTVETVQAAKSAGNSRLEALQGVKAALSGAQAVQAGRLAD
                     AQGADAGNNNTVGISLSYGSQSSKSEQQSEQTVAKGSTLTAGNHLSIQATGSGVKGVD
                     GDLTIQGSQIKAGNNVLLQANRDVNLVSAENTSKLEGKNTSGGGSVGVGIGVGAGGWG
                     ISVSASANQGKGSEKGNGTTHTETTVDAGKQLTIISGRDTTLTGAQAGGETVKVDAGR
                     HLTLTSEQDSDHYDSKQQNASAGGSFTFGSMSGSASVSLSRDKMHSNHDSVQEQTGIF
                     AGRGGFDVTTGQHTQLNGAVIASTATADKNRLDTGTLGFSDIENRADYKVEHQSVGIS
                     TGGSIGGQFAGNMANNLLVGANHEGHADSTTQSAVSAGNITIRDTKSQKQDVADLNRD
                     AAHANQTLSPIFDREKEQQRLQMAQLTGEIGNQVADIARTEGQIAGEKAKRDPAALNQ
                     ARAELEAAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQAIQAATAAVQGLAGGNLSA
                     ALAGGAAPYIAEIIKQTTPDGAGRVAAHAVVNAALSLAQGKNALAGAAGAATGEVVGM
                     IATQMYGQPVSELSETEKQTVSTLATVAAGLAGGLVGDSGASAVAGAQSGKTTVENNA
                     LSLPSGMVSYGQAVASWNQYADANNLTPEQKQAGLDKLAKGELPEGANISKVIVDGYK
                     DGVLIAGAWYLGPAVSVGKAAVGGVTSAAVNGGFQWYGLSQPGSENQSWDYKGSASSF
                     MTGMLAPGRTVTANTAIALGGSVFSDGANIKSQSGTALGAYAGGYVGEYFPVILRPVL
                     GNSASTVGDIGGGFVSELINY"
     gene            complement(8912..9439)
                     /locus_tag="ACHKUB_003861"
     CDS             complement(8912..9439)
                     /locus_tag="ACHKUB_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO6656822.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKNRQYVVATEQGRPVFFMSQAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            complement(9449..10528)
                     /locus_tag="ACHKUB_003862"
     CDS             complement(9449..10528)
                     /locus_tag="ACHKUB_003862"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO6656823.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSLDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQATLDAGVSWQRGTRWFGAQPAPEEIFGEATALSKILQMNA
                     SFNLPFTLAEQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQELYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRGQLFSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFRDF"
     gene            complement(10526..11101)
                     /locus_tag="ACHKUB_003863"
                     /pseudo
     CDS             complement(10526..11101)
                     /locus_tag="ACHKUB_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
     gene            11814..12386
                     /locus_tag="ACHKUB_003864"
     CDS             11814..12386
                     /locus_tag="ACHKUB_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656824.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWTAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASPAPGHLKDKFIEPLVKRVKDVAEGRVR"
BASE COUNT         2671 a         3745 c         3091 g         3159 t
ORIGIN      
        1 cgtagtttat taactcagaa acaaagccac caccaatatc accaacagta cttgcagagt
       61 tacctaacac tggcctcaat atcacaggga aatattctcc cacatatcca cctgcgtatg
      121 ccccgagagc agttccagat tgggacttaa tattagcacc atcgctaaat acagatcccc
      181 ccagcgctat agctgtatta gctgtaactg tacgacctgg agcaagcatc cctgtcatga
      241 atgaagatgc gctaccttta taatcccatg actgattttc actaccaggt tggctcaatc
      301 cataccattg aaaaccaccg ttcacagcag cggaagtaac tccaccgacc gcagctttac
      361 ccactgacac cgcaggcccc agataccagg cacctgcaat caatacgcca tctttataac
      421 catcaactat aactttgctt atattggctc cttcaggtaa ctcacctttg gcaagtttat
      481 ccagtcctgc ctgcttctgt tccggggtga gattattggc atcagcatac tgattccagg
      541 aggcaaccgc ctgaccataa ctcaccattc ctgatggcag actgagcgca ttattctcaa
      601 cagtcgtctt acctgactgt gcgccggcta ccgctgaagc tcccgaatct cccaccagac
      661 caccggccag gccggcagca accgttgcca gtgtcgagac cgtctgcttc tctgtctcac
      721 tcagttcact caccggctga ccgtacatct gtgtggctat cattcccacc acctcgccgg
      781 tggctgcgcc tgcggcaccg gcaagagcat tcttaccctg tgccagagac agggctgcat
      841 tcactactgc atgagctgcc acacgtcctg caccatccgg ggtggtctgt ttgataattt
      901 cagcaatata cggcgcagca ccacctgcca gggctgcact cagatttccg ccggccagtc
      961 cctgtactgc tgccgttgca gcctgtatcg cctgctggta tttgcctccc gttccaaaac
     1021 ctgaggccgc cataccgtta ttgtaggccc gctgcgccac atcctgttcc gtgaacggtt
     1081 ttcctgctgc ctcaagctct gcgcgggcct gattcagtgc tgccgggtcc cgcttcgctt
     1141 tctcaccggc tatctgtcct tccgttctgg caatatctgc cacctggtta ccaatctcac
     1201 cagtgagctg cgccatctgc aaccgctgct gctctttttc cctgtcaaag atgggggaca
     1261 gcgtctgatt tgcatgtgct gcgtcacggt tcaggtctgc cacatcctgt ttctggctct
     1321 tcgtatcccg gatggtgata ttcccggcag acacagccga ctgagtggta ctgtccgcat
     1381 ggccttcatg atttgcacca accagcagat tgttggccat atttcccgcg aactggccac
     1441 cgatgctgcc gccagtgctg atacccacgc tctggtgttc aactttgtaa tcagcacgat
     1501 tttcgatatc actgaagccc agtgtgccgg tatccagccg gtttttatct gccgttgccg
     1561 tggacgcaat cactgcgccg ttcagttgtg tatgctgtcc ggtggtaaca tcaaatccgc
     1621 ccctgccggc aaaaatcccc gtctgctcct gtacactgtc gtggttactg tgcattttat
     1681 cccggctgag gctcacactc gccgaaccgc tcatggaccc gaaagtgaag ctgcctccgg
     1741 cactggcatt ctgctgttta ctgtcataat ggtcactgtc ctgctcactg gtcagtgtca
     1801 gatgacgacc ggcatccact ttcaccgttt caccgccagc ctgcgcgccg gtaagcgtgg
     1861 tgtcccgccc gctgataatg gtcagctgtt ttcccgcatc cactgtggtt tctgtatggg
     1921 tggtaccgtt gcctttttca ctgcccttac cctggtttgc gctggctgaa acactgatac
     1981 cccagcctcc ggcccccaca ccgataccga cacccacact gccgccaccg gatgtgttct
     2041 ttccttccag ctttgacgtg ttttctgctg ataccagatt cacgtcacgg tttgcctgca
     2101 gcagaacgtt gttcccggcc tttatctgac tgccctgtat cgtcaggtca ccatccacgc
     2161 ccttcacgcc ggagccggtg gcctgaatac tgaggtgatt acctgcggtc agtgtactgc
     2221 ccttcgccac cgtctgctct gactgctgtt ctgatttcga agactggctg ccgtaagaga
     2281 ggctgatgcc caccgtgtta ttatttccgg catcggctcc ctgcgcgtct gccagtcgtc
     2341 cggcctgcac ggcctgagcc cccgagagcg cggccttcac cccctgcagg gcttcaagcc
     2401 ggctgtttcc ggcagatttc gccgcctgta cggtctccac cgtcgtgttc agggcgctgc
     2461 ccactgtgcc ggacagggca agcgtcagac cggaggtttt ttgctccact gtatgcgtct
     2521 gcacatgccg gtctgtgacg gcgctaatgc tgacctcctt accactgagt gccatgtccc
     2581 tgccggcaat caggtcagca ctgttggcgc tcagccggtt acctgcctga agggaaacat
     2641 caccgtgaac actccccacc gtgctgcctt tctgtgtgct gtctgttgcg gtgtccgtga
     2701 ctttcagact ctggctgccc acgctgaatc ccacacctcc ggagctcatc agcccggatt
     2761 ttttctcctg tttcaggtgt gtttcacggt tgctttctgt cagcgtgccg atactcaggt
     2821 catttccggc agccagggag acacggttat ccccggccac actgctgccc gtgacgttca
     2881 gattatgtcc ggcactgata ctgaccgtat ccccgttcag ttcactgccc gtcatggtcc
     2941 ggcggtcagt gacatcatgc gtggttgtgg tggttttcga taacatcccg ctgctgccgg
     3001 tcactttatg gtattcatcc agcgagcggg tattctgtgc accggtcagc gtcacgtcat
     3061 gacctgccat cagggccagg cctttctgag ctgacagcga tgccgcctgt gacgtcatat
     3121 cccgtccggc ggtcagcgtg acatcaccgt taccggcaag cgtgctgccc gtactctgtg
     3181 tgatgccctg cgacagacga ttatttttat cccagataat attgtcctgc gcggaagtgg
     3241 tcactgtgga aagcgtcata tccctgcccg cactgagttg cgtcaggctg tccttaccct
     3301 gattcaccac cgttgcagcc gtcagattca tatcccgtcc ggcctgcagc accagtcttc
     3361 cctggtcgtt ctgcacatac acgccggcca cactgtcgat actggtacgt tcaaaatggt
     3421 tctgtccggc gtcgcgctgt gccgtacggg tggtggtggt cagactgata tcccgtccgg
     3481 ccatcgccag caggctgtcc gtggcctgca gcagcccgcc ggtactgtta atatccgtcc
     3541 gggccgtcag gctgacattt gctccctgaa tggagccggc ctggttgtga atgttttctg
     3601 cactgagctg cacggtctgc ttcccggcca gtttaccgct gttaaacagg tcaccatcca
     3661 gcttcatcac catgtccctg ccggcaatca gcgcaccggc actgttcaca tcaccgggtt
     3721 ttacacgggc atacacctga ggtacctgaa ctgtctgcgt gctgccgtcc ggcagtgtca
     3781 ccgtggtgtt aaccagccag acaatatccc cggtcagtaa cgccatctgc tcagcagtca
     3841 gcgccacacc cggtttcagg ttgtattgct taccaaatgc aatacccgca tccattaatg
     3901 ccttaaactg ctcttcatca ctgccgtatc cgtccaggaa ccgttgcccg gtcagggcga
     3961 caacctgctc acgaatcata cgctgttcgt aaaaaccgtc tcccaggcgt ttgagcatgt
     4021 tatccccgtt ctgagaaaac gccttctgca tgtaatcact gcccagccac gtcttctggt
     4081 tcgtaaaacg cgggtccgtt tcaaccagat atggcacatt cactgccgga tttgttttaa
     4141 acagactgct gtccggcaga cgggtatccg gcccggcaac atggatactg ccctgaggtg
     4201 tgctgacttc aaactgctgc cccggcaaca gaatgaccgg ttcaccggct gacaccaccg
     4261 ggcgaagcgg tatccggtca gtagcggcta cagtcgcatc cacatttccg gcctgtcctg
     4321 tctgtacatc cgttccctgt ggcttcaggg gagaaagcgt gacatggctg ccctgaacct
     4381 gtccgtggct ggtgagctct cccggcttca gcgtgatggt ctgaattaca gtgggcggag
     4441 tgtactgaga ggttttctcc ccctgcttat cctttccttt tttcctgatg cggtaataat
     4501 acgtggatgt tcctgagtcc gtaacatgcc gctcgccact gacactgaca ttttccacgc
     4561 ttcctgacgg aatgctcagc aggccgcctg caaccaccag gctcttgtca ttaagcagtt
     4621 tgtctgccac aaccgtcatg ccggcaccgg acagaatttt tcccgggtcg gagtctttta
     4681 cccgggtttc ttccacggta cgggtgtaat cgtactgcgt gaatttatct ctggcgcctt
     4741 ccggtgtatg cagttttttc agtgagttac tggaatcgtt atccacataa acgcccggtt
     4801 caccggcgct ccagcgttta tctgagccgc tgagctgata ctcagtcacc ttttccgtgg
     4861 aaaccaccac gcgctccgtg gtgaaatggt cattcacgtt attgatttgt ccggcgctca
     4921 gtgccaggga accggtggct tcaacagtgg cactgtgatt attcagtaca ccggctttac
     4981 ctgtggcgct gccattcgca tccagcgtgc caccgatatg catatcgcct gcactgtaaa
     5041 tcaggctgtg cgtccggtta ttcagggtct gtacccctaa gtccacacgc tcacgcccgg
     5101 ccagtgtcgc cgccacgcca ttttcttcca gattattaaa tgtggcagca ctgacaccaa
     5161 cagcatcgcc gtagattcgc ccggtaccgg aattcgtcag ggtgttcgcc tgaagacgcg
     5221 tatatttccc gtcaatcagc ccacggttca gcagtgtgtc cgtcacgtta agccaggtct
     5281 gcccggcact gatttcacct ttttctgtgt tttcaaggcg gatgctgttc agctcaagcc
     5341 ggctgcccgc cagcaggcta ccggtattag tgacatcgcc attaacggaa agcgtaaggt
     5401 ttccgttcgc aatagtctga ccactgttgc tgaacgctgt gttacttttc agcgttatat
     5461 cgcccggcga aagcagtttc ccgtcaccac caagctgccc ggctgtcacg gatactgact
     5521 gcccggcctt aaccacgccg tcacggttcg aaaacgccag ctgacggcca ctgacaaaca
     5581 atgtcgcgcc ggaagagagc acgccgtcat tattactgag cgtcctgtct gccataaccg
     5641 taagcgccct gtctgccaga atactgccct cctggttatc aaggtcctgc gtcgtgatgt
     5701 gtattgtctg cgcttccagc ccctggccgg tattctgcgt gtggcgattg ataaactgca
     5761 gtgcattcag cgtaatggcg gaaccactgc gaatcagacc agagacgttg tccacaacgc
     5821 cccgtacact gtcgagcagc atgtcgccca cagactgtat ctgtccgtgg ctgttctgca
     5881 gacctctcgt atgcagacgg atttcacctt cactgaccag ccggccaccc tcacggttat
     5941 ccagcgaggt ggtgctcagg tcagctgtcg ttttcgcccc catggtcccg ccccggttat
     6001 ccattgctcc ggtactgacg gtcagttcct gcaggctgtt cagcgtaccg cgtctgttat
     6061 ccagcgtccg gcctgcggta ttaatgctga tacctttccc ctgaataaga ccggactgat
     6121 tgttcagcgc cagactgttc cggccaggca gaccgttcgc cacaatctga ccactcgcta
     6181 caatctgacc ggatgtattg ttaaccacgc ctgcagtcag ggacagcgca tcacccgaca
     6241 aaagcactcc ggcggtgctg tccagtgtgc cggcattcag tgtcaccggg ccctgactga
     6301 taaccccgcc ccggtcacca ctgttacggt tgctcagtgt gtcagtacgg agattcagcg
     6361 atgcaccgct ctgtatcagg ccgccggcat cgttgatgat atccgtcgag gaaagggtca
     6421 gtgactgctg tgccaccagt ctgccgccct gattatcaga gcgggtgaca tccagtctgc
     6481 tgttaccgtt gctgaccacc gtcccctgct ggttttccag cacattcagg tgcgcattca
     6541 ggtctccgcc ggcggcaagc gtcccttcct gattactgac atcgtcacca ctcagcaaca
     6601 tatccgtccc ggccttcagt gcgcctttct ggttcatcag cgtacctgac gatacctgta
     6661 gtgacgcctg gctccccagc gcgccctgac ggttatcgag cgttccagtg ttcagggtca
     6721 catccttctg acccgcaata aatccctgct ggttattcag cataccggcc cgtactgtca
     6781 gggtaccggc gctgctgata ccaccccgtg ttttcgtgtt ccggttactg agtaccccgt
     6841 cctgtacatc cagcgtcatg tcactgccgg actgaatcag accatcatca gagaccagtt
     6901 cgctgcctga cacagacagc gttttctgtg aaagcagtct gccggacgta ttatccagcg
     6961 caccatccag cgtcacggac agcccgtcag acaccaccgt accctggtgg ttattcagac
     7021 tccctgccga aatatccagc ctgccggcgg actccattcg tccctccgta ttgctgacag
     7081 actgctgtgt ttttatcctg cccgttccgg cagcagctga aatcagtccg ttgtcgttat
     7141 tcagcgcctg tgtattaatc aggatgtcac tgctggcaag cagacgtccc tgctggttat
     7201 ccacggcacc ggttgtcttc acctgcagac tgcctgactg cgccgcctgc actttccccc
     7261 tgcgattatc aaggctcagt gcatccaggc gcaccgcgcc atttgccgca agcagccctt
     7321 cccggttatc cacgcttccc ggcagactca gcgaaaaatc ccctgttccc gtctgaatca
     7381 gactgccttt acgatttgac aggctttgtg ccgatatctg cagtgtgtcg gcatttatcg
     7441 tgccgtcatt attgctgaac tgcccggcag tccgggcgga aagcgtaccg gcgctcagct
     7501 gcgcattctg tgtggacaga tttccgctgc ctgcatccag cccggtatgt tgtccctgta
     7561 tccggctgtt gcttaaatcc agcccctgtc ccttcagttg catgttccca ccggacagga
     7621 cctgtccgtg cgccgccagt acgccggtgg tactgacggt caggttgccc gcttccgcca
     7681 gacgtccgtc atctttcacc ccggcggcca gcagactccg ctcactgccg gtcagacgcg
     7741 ttgtctgaat ccgggtgttg cgacgtgatg ccacagagcc agtatgggtg aatgccccat
     7801 cactctgtat ctgcgtatcc tgccccgcat aaacagaacc actgttatgc agctcccggg
     7861 ttgtcgccag atgcacatcc gttttcgcac tgatactgcc actgttttca atacgtccgt
     7921 cagccgtcag ggtaaccgcc ccggcctgcg caccaatatg accggcgtta cgcacgccca
     7981 cacctgtttc cgtaccgaca aggcgaattt ttccggcata cattccacca agactggaca
     8041 catccagcgc cacctgtggc tgcgaagagg catcagcggc ttttttctca gtctgcccgt
     8101 gcgtaatatc cacattgtta cgtccggtgg tcactttcag gtcctgcgcc cagacacctg
     8161 cattaatctt cactgaccgg gcaatgatat cggtataatc cgtgcgcgag gcatccatcc
     8221 ccttgcccgt gacctgaatc tcaccacctt caacacggaa tccggtcaga ctgccgtttt
     8281 tcatctgcgg ctgccccgtg gtcagcgtgg cccggttcgc attaataaag ccgcacccgt
     8341 cgcaactgat accggccggg tttgcaatca ccacctgcgc ttttttcccg gcaacctcaa
     8401 caaaaccgtt caactggctg ggattgcgac tgttcacttc attaagaatg attctggcct
     8461 ctcccttagc cagccacggg ttcccggcca ccatgccacc caactgagtc tgcacattgt
     8521 tatgggcatt attcagaatg acaccctggt taccgacgtc gaactggctg tatgtgttat
     8581 gtgacacccc gccggcagaa ggcgtctgaa tattcacctg cggaatgccg ctggccgtct
     8641 gcataatcgt gggctgctga tgccccggtg cgccatggtc tgcgacaatg cctgcagcgc
     8701 tgacagaatg caccatcccc gaggccagcc acagggagaa tgcaagcggc gtgacacggc
     8761 agatacgttg cctgcgtgga tatcctgttc gggaggaggg tgatgcaccg gcgcgaccgg
     8821 aacgggcaat atctgccacc accatcagca ttccccgggc tttattaaat atgatgcggt
     8881 aacaatttct gttcattctg ataaacctga cttatctctc cggcacatca acggcaagcg
     8941 ggtgctgcac acgccactgt cgcgcctctt cacgactgac ctgcgcaccc cggaaattca
     9001 tcacccgcaa cccggtctgc tgcccacggt ggtacagcga acgccagcac agacggggaa
     9061 aaatatcctg ccggacaaga cgactcatat ccggagtgtg tccaaaaggg gcaacccagt
     9121 cacagaccca catccggtcg ccgctgttcc agtccgactg aggcatgaga attgccggct
     9181 gcgtaagata acgcgcctct gcctccgggc tgagccaggc ctggctcatg aagaaaacag
     9241 gtcgcccctg ctctgttgcc acaacatact gccggttctt aatcaccggc agcagcaggt
     9301 ccggcagcgt atgcagtggt gcatcacgat gcagggggga gtgcatccac aaccagacgg
     9361 ccgcccccag gacttccgcc tcattgaccg gctcaccgga atacagggga ctcagaacgt
     9421 catatttccc gttacgcatg aaactttctc agaaatcccg gaacagatta aaaccggctg
     9481 tcacatcact cgtcataaaa cccgccggtt ttgaaaacgg acgaccgatg aaaacgtcat
     9541 aatctgtact gaacagttgc cccctcagcc ccacaacacc accggccaga tgacgtccca
     9601 ccaggtattc actaccggct ccccccacct cgccgtaatc cattcccaga tataactcct
     9661 gctccggcag tggcgtacgc caggccaggt cgttgcggat ataccagccg ttactggcat
     9721 tcagcgttcg ctccccgtca aaaccacgaa ccgtccagcg gttaccgatg gcaaactgtt
     9781 cctgtggggt cagcggggta ttgctcagct ggcgctgata ccggaaggaa taactgaagg
     9841 gctgctctgc cagtgtaaag ggcagattaa aggacgcatt catctgcagg atcttactga
     9901 gcgccgttgc ctcaccgaag atttcctccg gtgccggctg tgcaccaaac cagcgggttc
     9961 cccgttgcca gctgacacca gcatccagtg tggcctgaga aatataatga cggtgctgca
    10021 gcccaagtcg ccaggccgct gtctgccggc gctgcacctg gatttccgta tcattaatgt
    10081 agttacgcga cctgcgggcc tgaacatcgt aagtcagcac cgttttctgc gtcccgttac
    10141 ggtgcagtac ccggctcagc tgcatgccca gattctgact ctcgccactg tactggtaat
    10201 cctcaaccgc accagctacc gtctgatggt aatcgtaatc actggcggtg acggaaaact
    10261 gccagtaacc gaaaggcaca gagtaatggg ccgtgtagtt tttactgccc ttacccccgt
    10321 aatgtggaaa attatgggtg gcagaaacat acagcaggtc actcagggaa aaaggattat
    10381 ccagagaaaa tgttatacca ccgagataac gccctgtgct gcgggtacct gaatcatcaa
    10441 gcgaaaaccc cagtcgccag tatttgtcct gtttgcgggt aataagaatg tcactttccc
    10501 ctggctgctc accgggtaca atttccatat cagcctgcac acccggcagt cgttgcatat
    10561 tctccagccc ctgctcaatg tcccgtaaat ccagcagaga gccttcgtgt gccgggaatg
    10621 aggaatacaa ctgaatatag tcaccactgt ccggtgtcag acgcacatgt cgcacaacac
    10681 cgggaataat aaccagccgg aggataccgc tttttaaatc ctgcgaaggt gccagaacac
    10741 gggtggtgac ataaccatga tcgaccagac ggttctgcaa tgtactcatc agcagattaa
    10801 ttcctttcgc ccccatgcaa tgcccgaccg ccccgttggc gattttttgt aaaggcaacc
    10861 agtgtggtaa cgcatcagcc ccctccagtt ccgtctgttt aatctgaaaa cagggcgttt
    10921 caacaggaaa attaatctta cgggcaaaag agccaggtgc agaaaggcga acatcagggg
    10981 ctgaaggcgt cagttgttgc tcaagggctt cctgacgctg ctgctgaatg ataaatgggt
    11041 catttccatt atgccgggta tcagcagcct gaagatatga aggcaataat aaagcaggta
    11101 tcagtgtcag tgataaaatg aactgacagc gccggaatac gcgttgctct tcagatacca
    11161 cgcctctgtg acataatttt ccagccaaac gaaaaacctt ttatgcatgc tgtgtcaaca
    11221 atcacctgtc agcctcctga cgatgtctgc cgccattttg gcaataacac actaccgccc
    11281 ttccggaatt tcccgtatca accggtacca gaatgctgag taaccagtca gccaaatcag
    11341 gctggtttaa ttttgctcat tatagaataa tgatctgaaa aatatcaata taacaggtgt
    11401 atcgatcgta attgcagatg cttgcagatc tttattccag tatatgttgt taaactggaa
    11461 tatttagaaa tatatatcca tgactaatgt gaaaatacag aaaattattc cagtgaatgc
    11521 tttttatacg gtctcctggc acagaaaatg gtgatttttg atttgaatca ttgaaatcag
    11581 ttctgctaat aagaagacat gctgaaaagc cgattttcag ggggggattg aagggattgt
    11641 gagtttttcc ttcggctttt aacgtaactt cctgttttcc tgatattcca cggcaagatt
    11701 aatttttagc ggagcagaat gaataaaatc ttcatctggt gtttttgttg cgcgtaaatg
    11761 caggtgtaac tgtggatgtt atccactgaa gagctgctga tgaggatgaa gacatgctga
    11821 ctaaaaaaat gttactcgtt tgtgcttcgt gtgttgcgct gagtgcctgt tctgcacagg
    11881 aaattgcaga gctgaacagg cagataagtg acggtgcatt tgctgtgaca aaagtactga
    11941 acgtaggtgt aaaccaggca gatgatgatc tcaaatatct gtctcaggca ccccgggcaa
    12001 aaagagagaa acgtgatcat gtcatagaag tccctgttga tgtggataca gctgcgggaa
    12061 gactgaaacg gtactataag ttttattcaa atgaagaagt cgatgcgatt cgtaataacg
    12121 gacatgcgaa cgggaagtgg accgcagcag caataagtca gaacggacat atgtgggatg
    12181 ccatgcccgg tagttattat aaaatgggta gtgactggga tggtggcgaa tttgatgatc
    12241 atctgacgct tgaacttgaa aaaaatggca aaggaagtcg gatatatata acgtatgcat
    12301 ctccggcacc ggggcatctg aaagataaat ttattgagcc attagttaaa cgtgtgaagg
    12361 atgtggctga aggccgggtc aggtaacgtg tactatataa tatatcccct ggcatacata
    12421 ctgccagggg atgttgcttt tctgtatgtc attttatgat gatacctttg atattcttat
    12481 agatgtttag gtgaactgtt aatgataaat attgtccgat attatacttt cttgtaagta
    12541 tattttgggg aggttatttt tattaaggtt ttaaatgttt tattttgttc gctttttctt
    12601 tctctgtttg ttgtttcttg ctattacaac tatcttgtga ttgtttatat gctaaacata
    12661 attctg
//
LOCUS       DAZFRN010000139        12547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFRN010000139 DAZFRN010000000
VERSION     DAZFRN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.141"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..286
                     /locus_tag="ACHKUB_003865"
     CDS             <1..286
                     /locus_tag="ACHKUB_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6656825.1"
                     /translation="RQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFR
                     CQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(326..1540)
                     /gene="ydcO"
                     /locus_tag="ACHKUB_003866"
     CDS             complement(326..1540)
                     /gene="ydcO"
                     /locus_tag="ACHKUB_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO6656826.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPMLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1593..2129
                     /gene="sutR"
                     /locus_tag="ACHKUB_003867"
     CDS             1593..2129
                     /gene="sutR"
                     /locus_tag="ACHKUB_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO6656827.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2202..4163
                     /gene="rlhA"
                     /locus_tag="ACHKUB_003868"
     CDS             2202..4163
                     /gene="rlhA"
                     /locus_tag="ACHKUB_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO6656828.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4255..4485)
                     /gene="yncJ"
                     /locus_tag="ACHKUB_003869"
     CDS             complement(4255..4485)
                     /gene="yncJ"
                     /locus_tag="ACHKUB_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO6656829.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4707..4861
                     /locus_tag="ACHKUB_003870"
                     /pseudo
     CDS             4707..4861
                     /locus_tag="ACHKUB_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4907..5323
                     /gene="hicB"
                     /locus_tag="ACHKUB_003871"
     CDS             4907..5323
                     /gene="hicB"
                     /locus_tag="ACHKUB_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO6656830.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5402..6808
                     /gene="ydcR"
                     /locus_tag="ACHKUB_003872"
     CDS             5402..6808
                     /gene="ydcR"
                     /locus_tag="ACHKUB_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6656831.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7053..8198
                     /gene="ydcS"
                     /locus_tag="ACHKUB_003873"
     CDS             7053..8198
                     /gene="ydcS"
                     /locus_tag="ACHKUB_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO6656832.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8216..9229
                     /gene="ydcT"
                     /locus_tag="ACHKUB_003874"
     CDS             8216..9229
                     /gene="ydcT"
                     /locus_tag="ACHKUB_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6656833.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9230..10171
                     /gene="ydcU"
                     /locus_tag="ACHKUB_003875"
     CDS             9230..10171
                     /gene="ydcU"
                     /locus_tag="ACHKUB_003875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6656834.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10161..10955
                     /gene="ydcV"
                     /locus_tag="ACHKUB_003876"
     CDS             10161..10955
                     /gene="ydcV"
                     /locus_tag="ACHKUB_003876"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6656835.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10977..12401
                     /gene="patD"
                     /locus_tag="ACHKUB_003877"
     CDS             10977..12401
                     /gene="patD"
                     /locus_tag="ACHKUB_003877"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO6656836.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3025 a         3086 c         3373 g         3063 t
ORIGIN      
        1 ccgccagctt gagtacaagc agctctggcg tggcggtcag gtgcttgctg ttccgccagc
       61 gtacacaagc cagcgttgcg cgtgctgtgg tcatacagcg aaagaaaatc gcctgtcaca
      121 aagtaaattc agatgccagg tatgtggata tacagcgaac gccgatgtaa atggcgctcg
      181 taacatttta gcggcggggc acgccgttct tgcctgtgga gagatggtgc agtcaggccg
      241 cccgttgaag caggaaccca ccgaaatgat tcaggcgaca gcctgaacgt agcagggatc
      301 ctcgtccttc agggcgtgga ggatgtcaat atcggtttct gtcagcgatt aaattcaaca
      361 ccacgtaaca aacgcctccg gcaattaatc cccagaacgc agaaccaatc ccgaccagcg
      421 tcaatccact tgccgttacc agaaatgcca ccaccgccgc gtcgcgctca cgctcattat
      481 gcagcgcctg atacaaactg ccgctaatgg tacttaacag cgccagacct gccagcatct
      541 ggatccaact tacgggcagg gcagccatca tcccggtaat agcactacca aacagacctg
      601 cgatcaaata aaaaatgcct gcaacggcag ctgccagcca acgttgatct ttatccggat
      661 gcgcttccgg gctttggcaa atagccgcgg tgattgccgc aataccgacg gaataaacgc
      721 cgaaagggga aaaaaccagt gccagcaatc cagtaaatac aattaatggc gaaacaggag
      781 ccgaataccc ggctgctttc attgctgcga tacccggtgc gttttgcgat gccatcgtca
      841 ccagaaaaag ggggagtgca acgctcaggc tgtgagcaaa cgaaaaatca ggggtaatat
      901 aagtggggag aacgggttta aagacaacat cagttgtgac aacgtcacct tgcgcgataa
      961 cgatcacgat cccaataatc atcgcggcaa ttaccgcata gcgcggcgca acggccttgg
     1021 ttgccagcca taccagcaac atacttccac acaacgtaaa ttgaccgtcc agactggcaa
     1081 acgcctgtaa accaaagcgt aataaaatcc cggcaagcat tgccgccgca agcgagtgcg
     1141 gaataatgcg catcagacga gcaaagagtc ccgttatgcc gcagaggact attaacgcgt
     1201 tggtgacaat aaaaacgccg atggcttcgt taagtgttag tccctgcaat ccggtgacca
     1261 acaaagccgc gccaggcgtt gaccatgcgg tgagaacagg tacgcgatac cataatgtca
     1321 gagtcagcgt actgacgccc attgccagcc ccagcgccgt catccagcca gagatttgtg
     1381 cagtggtggc tccggcgaca atcgctgctt gccagattat tgccgctgaa ctggcgtagc
     1441 caattaatac cgccagaaac cccgccagta gcatgggtgg aggaatagaa aacagacgca
     1501 tagtcacctc gtgcgttata gcgtacaggc accgtagcat ttgtccgtta taacgcacaa
     1561 gtgataaact tccgttttgc cggaggagtc gcatggaaaa tctcgctcgc tttttatcca
     1621 ccacacttaa acaactacgc cagcagcgcg gctggagtct ttcgcgactg gcagaggcga
     1681 cgggcgtttc taaagcaatg ttagggcaga ttgagcgtaa tgagtccagt ccgacggtcg
     1741 cgacattatg gaaaattgcc accgggttga acgtaccatt ttcaacattt atttctccgc
     1801 cgcagtccgc tacgccttca gtttatgatc cacaacagca ggcgatggtg atcacttcgc
     1861 tgtttcctta tgatccacaa ctctgcttcg aacacttctc gattcagatg gcatctggtg
     1921 caataagcga atcgacgccc catgagaagg gggtaatcga acatgtcgtc gtcattgatg
     1981 gacaacttga tctgtgcgtt gatggggaat ggcagaccct taattgtggg gaaggcgttc
     2041 gatttgctgc agacgtcacg catatctatc gtaatggtgg ggagcaaacc gtacattttc
     2101 attccctcat ccattacccg cgcagttaag cgggaaaact atttcgcaac gtcgcgcttg
     2161 tggctaaaat agccgccatt tttcagctac tggataagaa tgtgaccgta tcttctcatc
     2221 gacttgaact gttaagcccg gcacgcgatg ccgccattgc ccgcgaagct attttgcacg
     2281 gtgccgatgc tgtttatatc ggcggccctg gttttggtgc ccgtcataat gccagtaata
     2341 gcctgaaaga tattgccgag ctggtgccgt ttgcccatcg ttatggtgca aaaattttcg
     2401 tcacgcttaa caccattttg catgatgatg agctggaacc cgcgcaacgg ctgattaccg
     2461 acctctacca gaccggtgtc gatgcgctga ttgttcagga tatggggatt ctggaacttg
     2521 atattccgcc gattgaactg cacgccagta cgcagtgcga cattcgtaca gtagaaaaag
     2581 cgaagttcct ctctgatgtt ggcttcacgc agattgtgct ggcgcgagag ctgaatcttg
     2641 agcagatccg cgcgattcac caggccacgg acgcgaccat tgaattcttt attcatgggg
     2701 cactgtgcgt ggcctattcg ggtcagtgct acatttctca tgcgcaaaca gggcgtagcg
     2761 ccaaccgtgg tgattgctcg caggcgtgcc gtttgccata cacattgaaa gacgatcagg
     2821 ggcgggtggt ttcctatgaa aaacatctgc tgtcgatgaa agataacgat cagactgcca
     2881 acctcggcgc gctgattgat gctggcgtac gctccttcaa gattgaaggg cgttacaaag
     2941 atatgagcta cgtgaagaat atcaccgccc attatcgcca gatgcttgat gccattattg
     3001 aagaacgtgg cgatctggcg cgcgcttcat caggtcgtac tgaacatttc tttgttccat
     3061 cgacggaaaa gactttccac cgtggtagca cagattattt tgtgaatgcc cgtaaaggcg
     3121 atattggcgc gttcgattcg ccgaaattta tcggcctgcc ggtaggcgaa gtattgaaag
     3181 tggcgaaaga tcatctcgat gttgccgtca ccgagccact ggcaaatggt gatggcctga
     3241 acgtgatgat taaacgtgaa gtcgtcggtt ttcgtgccaa tacggtcgag aaaaccggag
     3301 aaaatcagta ccgcgtctgg cccaatgaaa tgccagcaga tttgcacaaa attcgtccac
     3361 atcacccact aaaccgtaat cttgatcata actggcagca ggcactgaca aaaacctcca
     3421 gcgaacgtcg ggtggcggta gacattgaac tgggcggctg gcaggaacaa ctgattctga
     3481 ccctcaccag tgaagagggt gtcagcatca cgcatacgct ggacgggcag ttcgacgaag
     3541 ccaataacgc cgaaaaagca atgaacaatc tgaaggatgg tctggcaaaa ctggggcaaa
     3601 ccctctatta cgcccgcgat gtgcaaatta atttgccggg ggcgctgttt gtaccaaaca
     3661 gtctgttaaa ccagttccgc cgtgaagctg ctgacatgct ggatgctgcg cgtcttgcca
     3721 gttaccagcg cggcagccgt aaaccggttg ctgatcctgc gccggtttat ccgcaaacgc
     3781 atctgagttt cctcgcgaac gtatacaacc agaaagcgcg tgaattttat catcgctatg
     3841 gtgtgcagct gattgacgcg gcgtatgaag cacatgaaga gaagggcgaa gtcccggtga
     3901 tgatcaccaa gcattgtctg cgctttgcct ttaatctgtg cccgaaacag gcgaaaggca
     3961 atatcaaaag ctggaaggcg acgccaatgc aactggttaa cggcgatgaa gtattaacgc
     4021 taaagtttga ttgccgccca tgcgagatgc acgtcattgg caaaatcaaa aatcacatac
     4081 tgaaaatgcc gttaccggga agcgtagtgg catccgtaag tccggatgag ctgctgaaaa
     4141 cattgcctaa gcgaaaaggg taaaacgcca gttttctggt tactcacaac tcattgaatc
     4201 tgcatgatat tgcctgccgg gtaaggcgtc tcgccgcatc cggcaacaaa tgactcaacg
     4261 ttgtcccgct tctggtttgc gcgatttttg ccagtaaaaa tgctcgcgca aaccttccgc
     4321 cgactcttcc gccacagcac gcaattcatc gctgtccgct tcatgacgca gctgatgatc
     4381 cacattcttt acccacacaa attcatgtcc tttgtgccct gccatgagtt gtcctgagaa
     4441 cagcgcacac gttaataaga caaccgataa cgccttcgta aacatcctgc cacctttttc
     4501 ttaccttttg ccgctatgat gccgatcgtt tcttgaggtt attattcagt tttgcaaatt
     4561 agcgcaaaga aattctggaa tcttccttcc tgattttgca tggcattctg ccgttgcggc
     4621 gatttagtgc tattttcgag caaattacac acggaggtaa acaataatgt ttactttgtt
     4681 gatatactca gcggcaggga ggcgatgtga aacaaagcga gttcagacgt tggctcgaat
     4741 ctcagggcgt cgatgtagcg aatggccgca accatttgaa actcaggttt catgggaggc
     4801 gcagtgtcat gccgcgtcac tcctgcgtaa agcaatcctg aaacaactcg gtttgagtta
     4861 atcgccaatt aaaaaggtta atgacatgcg agagacagtc gaaattatgc gttatcccgt
     4921 cactcttaca cccgcgccgg aaggcggtta tatggtttct tttgtggata tccctgaagc
     4981 gttgacgcag ggcgaaactg tcgctgaagc gatggaagcg gcaaaagatg ctttactgac
     5041 cgcatttgat ttttattttg aagataacga gcttatcccg ttaccttcgc cattaaatag
     5101 tcatgatcac tttattgaag tacctttgag cgtcgcctct aaggtattgc tgttaaatgc
     5161 ttttttacag tcagaaatca ctcagcaaga gttagccagg cgaattggca aacctaaaca
     5221 ggagattact cgcctattta acttgcacca tgcgacaaaa atcgacgccg tccagcttgc
     5281 ggcaaaggcg cttggcaaag agttatcgct ggtgatggtt taattacagt taacgaaaag
     5341 ttgtcatttt taacaactga tatagactgc cgaatcatct gcacataatt acgattcgat
     5401 aatgaaaaaa taccagcagc ttgcagaaca attacgcgag cagattgcgt cgggtatctg
     5461 gcaacccggc gatcgtttgc cttcgttgcg tgaccaggtg gcgctttcag gcatgagctt
     5521 tatgactgtc agccatgcct atcagttgct cgaaagtcag ggatatatta tcgcacgacc
     5581 gcagtcgggt tattacgttg cgccacaggc aataaaaatg ccgaaagcgc cagtcattcc
     5641 agtcactcga gatgaagcag tcgatatcaa cacttatatt tttgatatgt tgcaggccag
     5701 tcgcgatccg tcggtcgttc cgtttgcctc ggcctttccc gacccgcgac ttttccccct
     5761 ccaacaacta aaccgctcgc tggcgcaggt aagcaaaacc gccacggcga tgagcgtgat
     5821 tgaaaactta ccgccaggaa acgcagaact gcgtcaggct attgctcgtc gctatgcctt
     5881 acagggcatc accatttctc ctgatgaaat tgtcattact gccggggcgt tagaggcatt
     5941 aaacctcagt ttgcaagcgg taactgaacc gggcgattgg gtgatagtag agaatccttg
     6001 tttctacggt gcgttgcagg cgctggagcg gctacggctg aaggcgttat cggtggcgac
     6061 ggatgttaaa gaagggatcg atcttcaggc gctggaactg gcgttgcagg attatccggt
     6121 gaaagcgtgc tggctgatga ctaatagcca gaacccactc ggatttacct taacgccgca
     6181 aaaaaaagca caactggtgg cgttactcaa tcagtacaac gtaacgctga ttgaagatga
     6241 cgtttacagc gaactttatt ttggacggga aaaaccgctg cctgcgaaag cgtgggatcg
     6301 ccacgatggc gttttgcatt gctcttcgtt ttcgaaatgt ctggtgcctg gttttcgtat
     6361 tggttgggtc gccgccggaa aacatgcacg taaaattcaa cgcttgcagt tgatgagtac
     6421 gctttccacc agctcaccga tgcaacttgc gctggtggat tacctttcca cgcgccgata
     6481 cgacgcccat cttcgtcgcc tgcgtcgcca gcttgcggaa cgtaaacaac gtgcctggca
     6541 ggcactgctg cgttatctgc ctgcggaagt taaaattcat catagtgaca gcggttattt
     6601 tctctggctg gagctccccg agccgttaga tgccggcgaa ttaagcctgg cggcactgac
     6661 gcatcatatc agtattgcgc cgggtaaaat gttttctacc ggtgaaaact ggtcacgttt
     6721 ttttcgtttt aataccgcgt ggcagtgggg agaacgtgaa gaacaggcgg taaaacaatt
     6781 aggcaaactt attcaagaac ggctgtaata gcgtttaatt taattcctct tagattgggt
     6841 aatatgaatt tcgaatagca gtcatatttc ctaacccctt gtctatactc cagaagataa
     6901 ccttacagac ggcataatgc gcggtagctc acaacctgat taaattttct caggggcgaa
     6961 ggtgtgcctg caagccgccg tctatggtta aacaaggaga tatttttacg gcacggcggc
     7021 tgaacaatta attacgacag gagtaagacc ttatgagcaa gacatttgcc cgcagcagcc
     7081 tgtgtgcgct caccatgaca ataatgaccg ctcacgccgc cgaaccacct accaatttag
     7141 ataaaccgga agggcgactg gatattatcg cctggccggg atacatcgaa cgcggacaaa
     7201 ctgataaaca atacgactgg gtaacgcaat ttgaaaaaga gacaggctgc gcggtgaatg
     7261 tgaaaaccgc cgcgacttcc gatgaaatgg tcagtctgat gaccaaaggg ggttacgatc
     7321 tggttacggc atccggcgat gcctcgctgc gtttgattat gggcaaacgc gtgcagccga
     7381 ttaataccgc cttgattccc aactggaaaa cgctcgatcc gcgcgtggtt aaaggcgact
     7441 ggtttaatgt tggcggcaaa gtttacggca caccttacca atgggggccg aacctgctga
     7501 tgtacaacac taaaaccttc ccgacgccgc cggatagctg gcaagtggtt tttgttgagc
     7561 aaaatctgcc ggacggcaag agcaataaag gccgcgttca ggcttatgat ggccctatct
     7621 acattgcgga cgctgcgttg ttcgttaaag ccactcagcc gcagttgggc atcagcgatc
     7681 cgtatcaact aaccgaagaa cagtaccagg cggtgctgaa agtgctgcgc gctcaacaca
     7741 gtttgatcca tcgctactgg catgacacta ccgtgcaaat gagcgatttc aaaaacgagg
     7801 gtgtggttgc ttccagtgcc tggccctatc aggccaacgc cctgaaagcc gaaggccagc
     7861 ctgttgctac cgttttcccg aaggagggtg ttaccggttg ggctgatacc accatgctgc
     7921 atagcgaagc gaaacatccg gtttgcgcct acaaatggat gaactggtca ttaacgccaa
     7981 aagtgcaggg cgatgtggcg gcctggtttg gctcgttacc ggtagtgccg gaagggtgta
     8041 aagccagtcc gttattaggc gaaaaaggtt gtgaaaccaa cggttttaac tatttcgaca
     8101 aaatcgcctt ctggaaaacg cctatagcag aagggggcaa gtttgttccc tacagtcgct
     8161 ggacgcagga ttacattgcc attatgggcg gtcgctaact tcgctggggt gctttatgac
     8221 gtacgcagtg gagtttgaca acgtctcgcg gttgtacggt gacgtgcgcg cagtagatgg
     8281 cgtcagtatt gcgataaaag atggtgagtt cttctctatg ctggggccgt ccggctccgg
     8341 caaaaccacc tgcctgcgcc tgattgctgg cttcgaacag ctttccggcg gggctatctc
     8401 tatctttggt aaacccgcca gcaatctgcc accgtgggag cgggacgtga atactgtctt
     8461 tcaggactac gcgctatttc cgcatatgtc gattcttgac aatgtcgcct atgggctgat
     8521 ggtcaaaggc gtgaataaaa agcagcggca cgcaatggcg caagaggcgc tggagaaagt
     8581 ggcgttgggg tttgtacatc aacgtaaacc gtcacaactt tctggtggtc agcgccagcg
     8641 ggttgctatc gccagagcat tggtgaatga accgcgcgta ttgctgttgg atgaaccgct
     8701 cggcgcactg gatctcaaat tgcgtgagca gatgcagctg gaactgaaaa aactgcaaca
     8761 gtctctcggt atcactttta tcttcgttac ccacgatcag ggcgaagcgt tatcgatgtc
     8821 cgatcgtgtg gcggttttca ataatggacg cattgagcag gtcgattccc cgcgcgatct
     8881 ctatatgcgc ccgcgcacgc cgtttgttgc cggattcgtc gggacatcca acgtttttga
     8941 tggactgatg gcagagaaac tttgtggcat gacgggaagc ttcgccctgc gaccggaaca
     9001 tatccgcctc aacacccctg gtgaactgca ggccaatggc acgatccagg cggtgcaata
     9061 tcagggcgcg gcaactcgtt ttgaactgaa attgaacggc ggtgaaaaac tgcttgtgag
     9121 tcaggccaat atgacaggcg aagaactgcc tgccacgctc acgcccggac aacaggtgat
     9181 ggtttcctgg tcgcgtgatg tgatggtgcc gctggttgag gagaggtgaa tggcgatgaa
     9241 tgtattgcaa tcaccttcac gtccaggtct gggtaaggtg tccggtttct tctggcgtaa
     9301 tccggggctg gggctgtttt tactgctgct tggcccgcta atgtggtttg gcattgtcta
     9361 tttcggctcg ctgctgacac tgttatggca gggattttat acttttgacg atttcaccat
     9421 gtcggtaacg ccggaactga cgctggcgaa tatccgtgcg ctgtttaatc cggcgaatta
     9481 cgacatcatt ctccgcacgc tgactatggc tgtggcagtc actatcgcca gcgccattct
     9541 ggcttttcca atggcgtggt atatggcgcg ctataccagc gggaaaatga aagctttttt
     9601 ttatattgcg gtaatgttgc cgatgtgggc gagctacatt gttaaagcct atgcctggac
     9661 gttattgctg gcaaaagatg gcgtggctca gtggttttta caacatcttg ggctggaacc
     9721 actgctgact gcgttcctta cattacctgc ggtgggcgga aatacgctgt caacttccgg
     9781 gctggggcgc tttctggtgt ttctctatat ctggttgccg ttcatgatcc tgcccgttca
     9841 ggcggcgctt gagcgtttgc cgccgtcatt gttgcaggcg tcggctgatc tcggcgcacg
     9901 tccacgacaa acctttcgct atgtggtgct gccgctggca atcccgggta ttgccgctgg
     9961 ctctatcttt accttctcac tcacactggg cgattttatc gtcccgcagc tggttggtcc
    10021 tccaggatat tttatcggca atatggttta ttcccagcag ggggcgattg gcaatatgcc
    10081 gatggcggcg gcattcaccc tggtgccgat tgttctcatc gcactgtacc tggcgttcgt
    10141 gaaacgtctg ggagcgttcg atgcactctg aacgcgcacc gtttttcctc aaactggcgg
    10201 cctggggcgg cgtggttttc ctacattttc ccatcctgat tatcgccgct tatgcgttta
    10261 acactgaaga tgcggcgttt agttttccac cgcagggcct gacgctgcgc tggtttagcg
    10321 tggcagcaca gcgtagtgat attcttgatg ccgtgacact gtcacttaaa gtggcggcgc
    10381 tggcgacatt aatggcgctg gtgttaggga cgctggcagc tgccgcgctg tggcgacgag
    10441 actttttcgg caaaaacgcc atttcgctgt tactgctgct gcccattgcg ctgccgggca
    10501 ttgtcactgg tctggcgcta ttaaccgctt ttaaaaccat caatctggag ccgggatttt
    10561 tcaccatcgt ggtcggtcat gcgacttttt gcgtagtggt ggtgtttaac aatgtcatcg
    10621 cccgttttcg ccgcacctcg tggagtctgg ttgaggcgtc aatggatctt ggggccaatg
    10681 gctggcaaac cttccgctac gtagtgttgc cgaatctcag ttcggcgcta ctggcaggag
    10741 gaatgctggc gtttgccttg tcgttcgatg aaatcatcgt tacgactttt acggcaggtc
    10801 atgaacgaac gttaccgttg tggttgctca atcagcttgg gcgaccgcgt gatgtaccgg
    10861 taactaacgt ggtggcactg ctggttatgt tggtaacaac cttgccgatc ctgggggcct
    10921 ggtggctaac ccgcgaaggc gacaatggtc aataaccact gatacaggaa tatgctatgc
    10981 aacataagtt actgattaac ggagaactgg ttagcggcga aggggaaaaa cagcctgtct
    11041 ataatccggc aacgggggac gttttactgg agattgccga ggcatccgca gagcaggtcg
    11101 atgctgctgt gcgcgcggca gatgcagcat ttgccgaatg ggggcaaacc acgccgaaag
    11161 tgcgtgcgga atgtctgctg aaactggctg atgttatcga agaaaatggt caggtttttg
    11221 ccgaactgga gtcccgtaat tgtggcaaac cgctgcatag tgcgttcaat gatgaaatcc
    11281 cggcgattgt cgatgttttt cgctttttcg cgggtgcggc gcgctgtctg aatggtctgg
    11341 cggcaggtga atatcttgaa ggtcatactt cgatgatccg tcgcgatccg ttgggggtcg
    11401 tggcttctat cgcaccgtgg aattatccgc tgatgatggc agcgtggaaa ctggctccgg
    11461 cgctggcggc agggaactgc gtagtgctta aaccatcaga aattaccccg ctgaccgcgt
    11521 tgaagttggc agagctggcg aaagatatct tcccagcagg cgtgattaac gtactgtttg
    11581 gcagaggcaa aacggtgggt gatccgctga ccggtcatcc caaagtgcgg atggtgtcgc
    11641 tgacgggctc tatcgccacc ggcgagcaca tcatcagcca taccgcgtcg tccattaagc
    11701 gtactcatat ggaacttggt ggcaaagcgc cagtgattgt ttttgatgat gcggatattg
    11761 aagcagtggt cgaaggtgta cgtacatttg gctattacaa tgctggacag gattgtactg
    11821 cggcttgtcg gatctacgcg caaaaaggca tttacgatac gctggtggaa aaactgggtg
    11881 ctgcggtggc aacgttaaaa tctggtgcgc cggatgacga gtctacggag cttggacctt
    11941 taagctcgct ggcgcatctc gaacgcgtca gcaaggcagt agaagaggcg aaagcgacag
    12001 ggcacatcaa agtgatcact ggcggtgaaa agcgcaaggg taatggctat tactatgcgc
    12061 cgacgctgct ggctggcgca ttacaggacg atgccatcgt gcaaaaagag gtatttggtc
    12121 cagtagtgag tgttacgccc ttcgacaacg aagaacaggt ggtgaactgg gcgaatgaca
    12181 gccagtacgg acttgcatct tcggtatgga cgaaagatgt gggcagggcg catcgcgtca
    12241 gcgcacggct gcaatatggt tgtacctggg tcaataccca tttcatgctg gtaagtgaaa
    12301 tgccgcacgg tgggcagaaa ctttctggtt acggcaagga tatgtcactt tatgggctgg
    12361 aggattacac cgtcgtccgc cacgtcatgg ttaaacatta agttagatgt tagtggctgt
    12421 tataggacgg ccactatcgt tttaggttct gagtttaatg gcggttaagt attagaatga
    12481 ttacattaat aactagcgta aaaattaggc taaatgtttg atggcgattt ttcatcctgt
    12541 cacagtt
//
LOCUS       DAZFRN010000140        12280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFRN010000140 DAZFRN010000000
VERSION     DAZFRN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.142"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(37..189)
                     /locus_tag="ACHKUB_003878"
     CDS             complement(37..189)
                     /locus_tag="ACHKUB_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6656837.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHKUB_003879"
     CDS             complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHKUB_003879"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6656838.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHKUB_003880"
     CDS             complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHKUB_003880"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO6656839.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHKUB_003881"
     CDS             complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHKUB_003881"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO6656840.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5092..5454
                     /gene="queD"
                     /locus_tag="ACHKUB_003882"
     CDS             5092..5454
                     /gene="queD"
                     /locus_tag="ACHKUB_003882"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO6656841.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHKUB_003883"
     CDS             5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHKUB_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6656842.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDILQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHKUB_003884"
     CDS             6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHKUB_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6656843.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHKUB_003885"
     CDS             7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHKUB_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO6656844.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHKUB_003886"
     CDS             complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHKUB_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6656845.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHKUB_003887"
     CDS             complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHKUB_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO6656846.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHKUB_003888"
     CDS             complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHKUB_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6656847.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10839..>12280)
                     /gene="ygcU"
                     /locus_tag="ACHKUB_003889"
     CDS             complement(10839..>12280)
                     /gene="ygcU"
                     /locus_tag="ACHKUB_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO6656848.1"
                     /translation="AAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHGIYTLP
                     IPAAVVKLGSTERVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDGSAMNQ
                     IINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRSIGQFS
                     TLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITEVTVKI
                     FKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHFADGKC
                     VLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQ
                     ILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNM
                     YFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHG
                     SAWALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         2791 a         3393 c         3155 g         2941 t
ORIGIN      
        1 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcgcctac
      121 catcagcgtg aatcccagta ctgtaaaaca cagcactatg attgccacaa gggcatattt
      181 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      241 acgtaagcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      301 aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc
      361 agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt
      421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct
      481 ttttaagcca aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt
      541 atgggtatca ccgaccgata aatatccttc atcccataat gggtgatatt tcaggccatg
      601 tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa
      661 tacgccacgc tgaattgcca gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg
      721 caggccagca aaccacgttt gcgcattcag ttctttcagc gcccggttca tcggttcgac
      781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta
      841 gcgtgcttcc tgccaggcgg cgctttcggt agcgcggtac actttcaggt tgagcttgag
      901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt
      961 gaggatcacc ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg
     1021 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac
     1081 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
     1141 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1201 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
     1261 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
     1321 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
     1381 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
     1441 tcccaatgcg gttgccgcca agatgcagat tgtagcgacc cggtgcttta cccaccaggc
     1501 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
     1561 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
     1621 gcaggaaacg ctccgcttcc gccatcgcca gcgggcaagt cgggaatgac acgcaggcca
     1681 tcgagttttc acgctgcggc gtgacggcat tcattaaacc gctctctttg gcgatcttct
     1741 cgatcttcgc tttctcgctt tccggtacac cggcgatgat cagattctgg ttcgccgtaa
     1801 tgcggaaatc gcctttgtgg atcttcgcga tctccagcag gccggttttc agcggacgcc
     1861 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
     1921 tgcccttaac ccagccaata cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt
     1981 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
     2041 ccagcgtgta tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
     2101 ttgtcacgac ggcttcggcc accgccagcg tatgctccag cggcagatag ccaaactcgc
     2161 tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat agaaagccca ccgcccacca
     2221 gcaggttgaa gcctaccagc ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg
     2281 catgcagatc gatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
     2341 ggtaggtctg accaaggatc ggttcttcat cagtagtggc gactttttcc tggtcgagcc
     2401 agatctccgc atacgcgcgg gtacgaggca acagatgctc agaaatcttc ttcgcccact
     2461 cgtacgcttc cgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
     2521 tcatgtcgtt agctgtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
     2581 gtttgacgtt ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa
     2641 tgctgccata gatggtgttt tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg
     2701 taatcacccc acccggcaga cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct
     2761 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
     2821 tcagcaggaa gttgtcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
     2881 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
     2941 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
     3001 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
     3061 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
     3121 gacataaatg tgggcaccat cattgatcca gcgccacagc tccgcgccct gttcgcgcag
     3181 tttgtcttgt acgtaaactt tttctttttg gtcgcgcgac caggcaagat cgatacgtgt
     3241 cagcacgcca tctttgacgt agcgctgcca ctccacctga tacaggaagt cttccgtaaa
     3301 gtgcggatta ccaaagaaca gccagttttt acctggcgct tcgtcggcgg cgcgttgctg
     3361 cataaaggcg cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc
     3421 cgggttagcg ggcaggcgaa agttatcgtt atgttcgata aatacgcgga cttcgccctc
     3481 ttcttccacg cgatccgcga ggaagctgga ggcaccaccg gcacgggctc ggccttccac
     3541 gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga
     3601 ggcgatggaa tacaggcgcg gggtcagcgg gcgcagcaga ttaatcagcg cttcggcatc
     3661 cagttgcgcc ggagagaaac gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg
     3721 taacttcgct ttatcgccca ccagcggcag cagtgtttcg ctgcgggtaa gcgtggcgta
     3781 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
     3841 cagaggcaac gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc
     3901 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg
     3961 ctggtaacgc aggcccgagt cacctaagtc aatttcgata tggcgaacgt ctttttcaga
     4021 gttacgcccg gtaattttct ggttaacaga gaggctagcc accagcggcg cgtctttgct
     4081 gtacgggctg gtgtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc
     4141 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
     4201 ctggtattca acatcggcat cgacacggtc gagcaggcgt tcaccaccca gttccgccag
     4261 cttgctgtcg aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
     4321 aaacacggca aacgcggtgt tttccagctt tggcgctttt ttggagaaca ggaacttatg
     4381 cagcgcgacg gcttcctccg gcggttcccc ttccccttgc gttgacgtca ctacgatgag
     4441 cagtttttcg ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt
     4501 cagttttgct gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt
     4561 ttgcgaggcg gagataatag ttatacccgg catttctgcg gctggcgctg gcgtcgctgc
     4621 aagcgcagca ggctgctgat tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
     4681 ggtgggagtt aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg
     4741 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
     4801 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
     4861 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
     4921 caatagtctt taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc
     4981 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
     5041 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
     5101 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacacgt cccggaaggg
     5161 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
     5221 gtcgatccgc atacgggctg gattatcgat ttcgctgaac tgaaagcggc gtttaagcca
     5281 acttatgagc gcctcgatca ccattatctc aatgatattc caggtctgga aaacccaacc
     5341 agcgaagttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
     5401 gcggtgatgg taaaagaaac ctgtaccgca ggttgtatct atcgcggcga atgataagag
     5461 tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tctggatatc
     5521 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
     5581 ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg
     5641 aaatccccgg cagcaaaaat ctttccggcg ggcggttata tacccatgca ctcgcggaac
     5701 tcctcccgca atttcacctg accgcgcctc ttgaacgacg catcactcac gaaagccttt
     5761 ccctgttaac gccggatggt gcaacgacgt tttccagctt acagcccggc ggtgaatcct
     5821 ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa aaagaaggtg
     5881 tcgaatgcat ccccggtgcg acggtggatg cgctgtatga agaaaacggc agggtgtgtg
     5941 gtgtcatttg tggtgacgat attctccgcg cccgttatgt ggtgctggca gaaggtgcca
     6001 acagcgtcct ggctgaacgt cacgggttag tgactcgtcc tgctggcgaa gcgatggcgt
     6061 tggggatcaa agaagtgctg tcgctggaaa catccgctat tgaagaacgt tttcatctgg
     6121 agaataacga aggcgcagcg ttgctgttca gcggcgggat ctgtgatgac ttacccggcg
     6181 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccactctctt
     6241 cccttacgca aagtcgtgtt ccggcaagcg agctgctggc tcgctttaaa acgcatccgg
     6301 cagtgcgccc gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
     6361 aaggtggctt gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
     6421 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga
     6481 ctggcgcaca ggcggcggca caaacgctga taagcgcctg ccagcaccgc gagccgcaaa
     6541 atctgtttgc gctttatcat cacaacgtcg agcgcagcct gctatgggat attctacagc
     6601 gttatcagca tgttccggcg cttttgcaac gccctggctg gtatcgggcg tggcctgcgt
     6661 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
     6721 gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta
     6781 tcaggagtct gcgatgtctg tagcccataa tctctggcgc gttgctgatg cgccgcacat
     6841 tgttccggct gactccgttg agcgtcagat ggtacaacgg ttgattaacg cctgtccggc
     6901 aggtcttttt tcgctcacac cggaaggtga cttacgcgtt gattatcacg gctgcctgga
     6961 gtgtggcacc tgccgtttgc tgtgcgacga atccacacta caacagtggc gctatccgcc
     7021 ttccggattc ggcatcacct accgctttgg ataaataata aggataattt atgcccctct
     7081 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
     7141 tggctatcga ttctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca
     7201 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
     7261 tggaaggcgc gtcaaataaa gaagtcgtta ttcagttttt gaaactggtg accgaggcgg
     7321 acggcattat cagtaccaaa gcctcaatgt tgaaagcggc aagagcggaa ggttttttct
     7381 gtattcatcg cctgtttatt gttgattcaa tttcgtttca caacattgat aagcaagttg
     7441 cgcaatcgaa tccggattgt attgagatcc tgccaggctg tatgcccaaa gtgctgggct
     7501 gggtgacaga gaaaatccgc caaccgctga ttgccggtgg gctggtgtgc gatgaagaag
     7561 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggggtct
     7621 ggacgttagc gaaaaaatta ctttgacggg ataatcgtaa ccaattgaat ttggtttgat
     7681 ttgtaggccg cacgccacat ccgacattca gcgcctgatg cgacgcttga cgcgtcttat
     7741 caggcctaca agtccccagg ccagaaccgt aggcaggatc atccggcaac acgttattgg
     7801 caatctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
     7861 ccaacatctg cctgtgaaaa taccgcagcg ctggcgtcat gattaatcgc caccacaaac
     7921 ttgctattgc gtacgcccgc cattaacgct gctgcgccag aagcgccaac aacaatacac
     7981 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
     8041 ttcatcactc gcgcccgact gtagcccact tcggctccca gcttttctgc cagcatagcg
     8101 atttcctggt tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct
     8161 aatgggtcgc gagtaacatt tttcagatcc tctgtgctaa ccagccagtc gggaagcgca
     8221 cccgggacaa tgttcagttg ttgcattcca gagggcaacg tggcgttttt cgctgctccc
     8281 gcctgacgcg ccagggaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc
     8341 gcatttcccc agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg
     8401 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
     8461 cccggcggaa agagaacaac gtccgcaggc gttcgctgcc actgttccac caacgcatcc
     8521 agcacctgtt ccgccactac aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg
     8581 ctgaaatcct gcgccgccag ccagcttgct attgcggcat tctcctgatt tattgtcaca
     8641 attgcgatat tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgagctt
     8701 tttctgctac cgtttgccca tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca
     8761 gttgcagaca ctgcatcgct ggcatttcgg cagcaacagt tttacggata atttctgctt
     8821 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
     8881 gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg caaaccatgt tcagtacgct
     8941 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc
     9001 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
     9061 agccggtaat tatcagatcc agcggattct gatgctgcca ttcggcaata tggcgagcga
     9121 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
     9181 caagagccat gagatagcgc agccagtgca atgcccgttc atcccccatg ctcaacgcgg
     9241 ttaaagacat cggcgtaccg ttttttctct gcgccaacag cagcgcggca gcggcctgtt
     9301 catcagcacc gagtaaactt cgcagtagcg aaatatccgg tccgctttta ccctgagccg
     9361 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
     9421 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct
     9481 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag
     9541 gtgacttgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
     9601 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
     9661 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
     9721 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt
     9781 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
     9841 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
     9901 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
     9961 gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    10021 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    10081 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt
    10141 agccagcgtg gtgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    10201 gctgccgatg ccagtagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    10261 gcaatacttg ccagcacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata
    10321 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga ataatcgcct
    10381 cccagaccaa tgccaatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa
    10441 aattgtaaaa acgaagcaag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    10501 ccaatatggt cggagatcca cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc
    10561 gagccgccga tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagctgaata
    10621 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    10681 gcagcaatgc ggcagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg
    10741 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    10801 aggcaacgcc ccgtcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata
    10861 gtacccgtat tcataatgcc attaggatcg aactgctttt tcagcccttc cagcaacgcc
    10921 cacgcgctgc cgtgttccag tttgctccag tgaacgcgat gtttaccgat accgtggtgg
    10981 tgcaccatcg aaccgccgag gcgaatggtt tcttcacaga tgatcttgtt gagcggattg
    11041 tggtacttgt cgatttcctc ttccggctta cagtcaacga cgttgtaatc gtagacgaag
    11101 tacatgttgg tgccgttctg atagctatga gaggaatgac cgcccagcat ggtgatgtcg
    11161 tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    11221 cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt gaggatctgc
    11281 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc
    11341 agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg
    11401 cccgtcgcct tcgcaatgcg aggattgcct tcagccataa agatcagcac acatttaccg
    11461 tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    11521 ggacgatacc cttccaccat gacttcacgc aggatgttga agccggtttt catgtcttcc
    11581 aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact
    11641 tcagtgatat agcaaagtgc accttcgtta ccgatgatga tgtggcgaat gtccgggcca
    11701 gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catcagccaa tactgcttcc
    11761 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    11821 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    11881 taaccttttt cacgcaacgc attttccagc acctccagcg gaacgccaca ttgcgccgtc
    11941 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggagcc gtcgagcacc
    12001 accgagtttt ccacaacggt ttccagccca ccttcagtgg cggaagcgcc ggtacgcggc
    12061 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggagactcg ttctgtggaa
    12121 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa taccatgaat atccggaaat
    12181 ttacgaaaac ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct
    12241 gcaccaacaa tttcctttag ttggtcgaca atcgctgcgc
//
LOCUS       DAZFRN010000141        12112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFRN010000141 DAZFRN010000000
VERSION     DAZFRN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.143"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(89..316)
                     /locus_tag="ACHKUB_003890"
     CDS             complement(89..316)
                     /locus_tag="ACHKUB_003890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656849.1"
                     /translation="MTNNTLSPIQDTQTQEDEIIRQRQSEACARLEEELTRTKIPPPA
                     PRLVPPEKFALEDFVDKYPRRLKSGKNRPPG"
     gene            complement(374..571)
                     /locus_tag="ACHKUB_003891"
     CDS             complement(374..571)
                     /locus_tag="ACHKUB_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6656850.1"
                     /translation="MSSGNTNNKSAKKNIRFPHELIDGIDASVEQEKLTNPSANFSAW
                     VLDACGRKLKYEQRRKAKEQE"
     gene            777..977
                     /locus_tag="ACHKUB_003892"
     CDS             777..977
                     /locus_tag="ACHKUB_003892"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6656851.1"
                     /translation="MRNNTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQGVGL"
     gene            1196..1456
                     /locus_tag="ACHKUB_003893"
     CDS             1196..1456
                     /locus_tag="ACHKUB_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656852.1"
                     /translation="MCNNIRPDATAAALTTLMLALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGLCVMIVLIP"
     gene            1429..1662
                     /locus_tag="ACHKUB_003894"
     CDS             1429..1662
                     /locus_tag="ACHKUB_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656853.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTKEYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            1655..1888
                     /locus_tag="ACHKUB_003895"
     CDS             1655..1888
                     /locus_tag="ACHKUB_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656854.1"
                     /translation="MRDIYIETIDRAFSALAYAEGMYEILQMWLETFGDNESDVKKAR
                     IIKSLITLLEPVITELQEIDLLHDRYNEQHTGE"
     gene            1894..2193
                     /locus_tag="ACHKUB_003896"
     CDS             1894..2193
                     /locus_tag="ACHKUB_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6656855.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQAVPRNVPTSREKARANIQKMKNMINGFRGKK"
     gene            2190..3590
                     /locus_tag="ACHKUB_003897"
     CDS             2190..3590
                     /locus_tag="ACHKUB_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO6656856.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     LPPIVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAAGVTDANY
                     YPEGIHSQKRENWREYLERERGKKPGEEHHQRKTTLPMSVGSAGYDTQLDYVVKGIIP
                     ASSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHGEQVKNLYLVNRPIFPAVPLDVDELVIAASQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            3791..4042
                     /locus_tag="ACHKUB_003898"
     CDS             3791..4042
                     /locus_tag="ACHKUB_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656857.1"
                     /translation="MTQKRRDRTEPKYKALDMAELALKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            4039..4344
                     /locus_tag="ACHKUB_003899"
     CDS             4039..4344
                     /locus_tag="ACHKUB_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656858.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            4354..4515
                     /locus_tag="ACHKUB_003900"
     CDS             4354..4515
                     /locus_tag="ACHKUB_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6656859.1"
                     /translation="MTAQIAAYGRLVDDPQVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            4820..5977
                     /locus_tag="ACHKUB_003901"
     CDS             4820..5977
                     /locus_tag="ACHKUB_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6656860.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            6033..6590
                     /locus_tag="ACHKUB_003902"
     CDS             6033..6590
                     /locus_tag="ACHKUB_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO6656861.1"
                     /translation="MKSMEIRSSEITTSASNTLTGYVVRWDNLSELLWGEFYEKFQRG
                     AFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTGRDVIE
                     LVKRGDISGMSFGFRSRKDVWDTTTEPCVRTVLVAELYEITVTSVPAYPDSGVELARR
                     SLYEQHPEKMPRADNRRWWVDLAGV"
     gene            6592..7803
                     /locus_tag="ACHKUB_003903"
     CDS             6592..7803
                     /locus_tag="ACHKUB_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6656862.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVVSPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            7800..8138
                     /locus_tag="ACHKUB_003904"
     CDS             7800..8138
                     /locus_tag="ACHKUB_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6656863.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            8135..8431
                     /locus_tag="ACHKUB_003905"
     CDS             8135..8431
                     /locus_tag="ACHKUB_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6656864.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTHVPMTIGALWTAYRDVGVY"
     gene            8431..8871
                     /locus_tag="ACHKUB_003906"
     CDS             8431..8871
                     /locus_tag="ACHKUB_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO6656865.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRSARQQQDSRRGSSRER
                     GYTRQWEKYRAMYLSKNPLCAHCLEKGIYTPAVVVDHIIPIDGGNDVLFWPEWNHQPL
                     CQACHNQKTKWLDPSTKNKRAAGGFREEEERAANRNNWMYGADE"
     gene            8855..9037
                     /locus_tag="ACHKUB_003907"
     CDS             8855..9037
                     /locus_tag="ACHKUB_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656866.1"
                     /translation="MALMNEREQNRLIRGLIRQRDAWKAQETGHKDKASGRAERITAT
                     RLNDRDREVMECFRNR"
     gene            9160..9516
                     /locus_tag="ACHKUB_003908"
     CDS             9160..9516
                     /locus_tag="ACHKUB_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO6656867.1"
                     /translation="MSRPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            9500..11161
                     /locus_tag="ACHKUB_003909"
     CDS             9500..11161
                     /locus_tag="ACHKUB_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6656868.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLESPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVMHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            11175..11456
                     /locus_tag="ACHKUB_003910"
     CDS             11175..11456
                     /locus_tag="ACHKUB_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656869.1"
                     /translation="MTEAEILGLIRRVSGISQQHDEQGTQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            11693..11845
                     /locus_tag="ACHKUB_003911"
     CDS             11693..11845
                     /locus_tag="ACHKUB_003911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656870.1"
                     /translation="MGGVADLQLDHEITETTRSLGSKSNTQILGLTRDTQIWGIGGNH
                     NDFRYG"
BASE COUNT         3238 a         2858 c         3395 g         2621 t
ORIGIN      
        1 tggctataaa agagcatagc ttactcttta tacacacaca aacgccccgg aaacggaata
       61 atccgtttca tagttcggtt ttgtgcactc atcctggcgg tcggtttttg ccggatttaa
      121 gccgccgtgg gtatttatcg acaaaatctt caagggcaaa tttttctggt ggcactaagc
      181 gcggcgctgg tggtggtatt tttgttctgg ttagttcttc ctcaagtctg gcgcaggctt
      241 ctgactgcct ttgccgaatg atttcatctt cttgcgtctg cgtgtcttgt attggtgata
      301 gtgtgttgtt ggtcatggta ctgccctgta aagcaatgcg ccgtagtacc tcacgccacg
      361 gcgctgatgg tggttattcc tgctctttgg ccttgcggcg ctgttcatat tttaatttgc
      421 gtccacaggc atctaagacc catgcggaaa aattagcact aggattagtt aatttttctt
      481 gttcaacgct ggcatcaatc ccatctataa gctcatgtgg gaatcgaata tttttctttg
      541 ctgatttgtt gtttgtgtta ccactagaca ttgatgcacc tcacttaaat ggactttatg
      601 ggcgcacaca atacagcaaa aaaaatgaga tgttaagtat tgacatgtgc gcacacatga
      661 ttttaatctg tgcgcacagt ttgagttttg tcactcaaat taagcaacgc cccgcagtgc
      721 tcgcaacaca tgcagggcgt ctaaccacca acgatagcga aagtatcgag gtagctatga
      781 gaaataatac cacacacccg caagggcggg actcgcacaa cctgaataaa tacatctggc
      841 gttttatcgc cctgagcacg gcacaaccgc gcgtgattac cattgaggcc accagcgaac
      901 aggaagcacg ccagcaatcc ccggctggct gcgtgatggt attcgctgcc cgtattcgcc
      961 agggggtagg cttatgagcc aggaaatcac actacaacag gcagcagaac gcgcccacca
     1021 aatcgaagtt atttgcgcac tggcagagga ttaccctggc atgatgaccg acagcgaatc
     1081 aggggcaatc atcggcttac ttaaacgcct tagcggtgag gtctgcgtat tcctgagcga
     1141 tgaacaggaa agaagaacgc ttatttctaa cgaaaaaaat gcggaggggt acaccatgtg
     1201 caataacatc cgtccggacg caaccgccgc cgcgctcact acgctgatgc tcgcgcttat
     1261 tgatatttct gttattgctg acagggcaca taagcacgcc accagtgaaa cagaatatgc
     1321 cggggctttc gttcctcatt cgctggcggt tatgcaactt agtgctgata tggcgctgaa
     1381 tgaggccaaa gctatcctga ttgctgattg tgaaaatggg ggggggttat gcgtgatgat
     1441 cgttttaatt ccctgaaaca agaattttcc ggcgttcctg atgatgcggc tgatgcgctt
     1501 tcgtcaatgc cagaacttat tagagcggct tttttcttac tttccacgaa agaatataaa
     1561 tcaacgggac ttgatgtact gaatatcgcc gccgattatg cggaatatgt ggcagaggcg
     1621 cgttacagaa gaaaatttcc tgaggatgta agccatgcgt gatatttaca ttgaaacaat
     1681 agaccgcgca tttagtgccc ttgcttacgc tgaaggtatg tacgaaatat tgcaaatgtg
     1741 gctggaaaca tttggcgata atgagagtga tgtaaagaag gcgcggatta ttaaatcact
     1801 gataactctc cttgaacctg taataacaga gttgcaggaa atagacttat tgcacgacag
     1861 atataacgaa cagcacaccg gagaataaaa ataatgaaac ttaaatattc tggcttaact
     1921 gccagtggca acactcaccc taaatttacg cgcggtgata tttaccgcga ccagtacggc
     1981 ggcacggtaa tgattaaggg cgtggcggga cggtgcgtaa cttaccgccg tgaaggttac
     2041 gaatatgatt gcgtgatgcc tgtttatcag ttccggcgtg atttttctct ggtacaggcc
     2101 gtaccgcgta acgtgcccac cagcagggag aaagcacgcg ccaatattca gaagatgaaa
     2161 aacatgatta acggattcag gggcaagaaa tgaaaagcgc accgaactta aaaaaacagc
     2221 cttacgacaa gatgaccgaa gtcatcattt ttgcgggtag tgatgcgtgg gcacatgcga
     2281 aacagtggca ggaacaggac gggcgactgg ctggcgataa tttgcctccc attgtgctgg
     2341 ctgatgatca actggatgaa ctggcagacc tgagaatcat cgacgagggg cgctattgtg
     2401 tccggctgta caaggcaggc cacatcaggc catcaaatat taatgccatt gcgcacaagc
     2461 tggcggcggc ggcgggcgta actgatgcga attattaccc cgaagggata cacagccaga
     2521 agcgggagaa ctggcgcgaa tatctggaac gggagcgggg gaaaaagcca ggggaagaac
     2581 accaccagcg aaaaaccacg ctaccgatga gcgttggatc tgccggatac gacacgcaat
     2641 tagattacgt ggtaaagggg attattccgg catcgtcgct atgcagcata tacggggcaa
     2701 gcggttccta taaatcattc cttgctggtt cgtgggcgtg tcacgtttcc acgggccgcc
     2761 agtggggagg ccgcagggtg gcgcatggcg ctgttctcta tgtggttggt gaaggcggta
     2821 tcggcgttcc gcgtcgtgta aaagcctggg aggttgtgca cggtgagcag gtgaaaaatc
     2881 tgtatctggt aaaccgtccc atctttccgg ctgtcccgct tgatgttgat gaactggtta
     2941 ttgctgccag tcaggtggag agggaaacgg gtaaacctgt tcgcatgatt attctggaca
     3001 cgctggcgcg ctgctttggc ggcaatgatg aaaatgactc ccgcgacatg ggggcgttta
     3061 tccgtgggtg tgacgaactg aaacgacgca caggggccac ggtgctggtg gttcaccatt
     3121 ccggcaagga tgagacgaaa ggcgcgcgcg gttccagtgc atttcgtgca tcactggatg
     3181 ctgaataccg gatacgcagg gaggacgcag gaagcgaagc gctggttatc tcatgcacca
     3241 aaatgaagga cgcggaggaa ctcaaagagg ctgcctatga tttgcgggtg gtggagcttt
     3301 ttaccgacgc tgatggggag ttaatcacgt cgctggtggt ggtggataag ccgcgccctc
     3361 ccgttgaact ggagcgcatc gaggaggcag ggaacaagac ggaaaaccat accgcgctat
     3421 gggggtgcat ccgttcacgc acacagaacg gcgacaagtg cacgatcccg ctgttacgtg
     3481 atgacatgaa aaaactgggg tatgaaatga aaaacttccg gcgctggctg tacaagctgg
     3541 aaaaagatgg ggttattcgt atcgatgggg atgatgtagc gccgctataa aagtgaggag
     3601 caaaagcgag gggggtagaa agagggccaa aattagcctg ctctccctca cttttcgacc
     3661 tgtatacatc ctcaaaagtg aggggtaaaa aaatacttat gaaacacacg catagaaaaa
     3721 ccaaaaatcc caactgcgac gaagtgagac gcttgaaaaa gtgaggcgaa aaagtgagag
     3781 gttgcgagaa atgacccaaa aacgcagaga cagaacagag ccgaaatata aggcgttaga
     3841 tatggctgaa ctggctttaa aggtggcaat cagaacgata gaccgccacg cgggggaagg
     3901 atacgcgaag gagcatcccg aactgataag cgcattcatg acaacagcgg cggcaaactt
     3961 tgccacgctg acagaacggg agattgccga agcggaacag gtaacaacca tcaacgttaa
     4021 aaccggagag gtggaatcat gagcgaacca aaatttggtg aaaaatttta caagcacaac
     4081 ggacggatca cgattctgca aataagcgca gctacaccag gctggtgggt tgaaacagat
     4141 gaaggatcct cacccgtggc atcatgggcg ctttgtgtgc ttagttaccc tgaccgcgat
     4201 gtgtatcagg atattctgcc tgtgatatcc actgacaaag gcatgaaacc cgtagacatc
     4261 aaaaacatgg ggtttcagtg cgtaatgctc actgaaaaaa tgatggaaga gatgaaaaag
     4321 aacaatgccg gatcgcttca ctgaggtggc aatatgacag cacaaattgc agcttacggg
     4381 cggctggtgg acgacccgca ggtaaaacag agcagcaagg gcacaccgga gccgccaacg
     4441 gagaactacg cagacctgag aaatgcagta aatcgcactt acgcagacat ggggctgagc
     4501 aaattagcag agtaggaata tcagaaccac accatacagc cggagcaatc cggctttttg
     4561 ttattttttg taaattattt gttcgtggtt gttccacgtt gttcactgag cggatcggca
     4621 tattttaccc gaactgaatc atgattattc tcgcccgtgg tgccaggacg ctggggccac
     4681 tttcccgcct gttaatgtgc ccgccaatat tcattaccag gcgggaaaac gatcggtgcg
     4741 attgctgatt tccttatgaa aaacggttga gtgtttgccg cgtcctggag ttccttactt
     4801 aaccccagga ctttttttta tgccgagaat aatcgaatta cgccagcaga aaaccgccat
     4861 taaaaatcag atgcgcgaca tgctggagaa cgctgaaaaa gaaaaccgca gtcttaacga
     4921 tgctgagggc gcaaaatttg acgaattacg cgctaaagct gaatccctcg ataaagacat
     4981 ttcccgcctt gaagccattg cagacgaaga gcgcagcaag ccaggtaaaa gcagtcagac
     5041 cactgacccc gcagaactac gaaactacat tctgacgggt gaaacccgcg cattaagtac
     5101 aggcgttccc gctgatggtg gttataccgt tatccccgaa ctgaacaccg aaatcatgcg
     5161 aatgctggcg gatgaatcca ccatgcgccg catctgtacc gtgaagaaaa tcagcagcaa
     5221 cgagtttaag cagcttgttt ccgctggcgg tgcgaccgtt aaccacggtg aagagggtaa
     5281 ggcacgcgaa cagaccagca ccccgcagat taacgaggtg agcattaagc tgtatccggt
     5341 ctatgcgtac ccgcgcacca cacaggaaat cgtggatttt tccgatgtgg acatcctttc
     5401 atggctgacg ggcgagattg gcgacacctt cacggaaacc gaagaaagcg atctggttgt
     5461 gggcgacggt gacaaaaaag caaaaggctt tttatccgta ccccgtgcag agaagaacga
     5521 caaagagcgt gattttggta cgttgcaggt aattaaacct tccgaatctc tggcgtggac
     5581 atctgcggac ccgctgatcg acctgaaatt tgcattacgt aaaaaatacc gcaagaacgc
     5641 ggtatgggtg gttaactcca cgacggcggc aaaactccag aaggtgaaga atgcgaacgg
     5701 ggattatata tggcgtgacc gtttacaggc gggtgatcct gatacgttgc tgggccttcc
     5761 ggtcgaatat ctggagttta tgccggataa cgttattgcc ctgggcgact ttaaacgcgg
     5821 ttactacatc gttgatcacg aaacaggtgt tcgcaccaga ccggacaacc tgacagaacc
     5881 aggattcatc aaaattttca cgcaaaaata tttaggcggt ggcgtggtgg attcgaacgc
     5941 gatcaagatt ctggaactgc cacaggacga cgattaacag catacagaag gggctaaaaa
     6001 gcccctttag tgttttatgg gtgaaaaaaa ttatgaagag tatggaaatc cggtcatcgg
     6061 aaatcaccac cagcgccagc aacacgctta caggctacgt tgttcgctgg gataaccttt
     6121 cggaactgtt atggggggag ttttacgaaa aattccagcg gggggcgttt actgagtggc
     6181 ttgcggcggg taatgacgtt cgcggcctgt atgagcatga ccacagcatg ttactggggc
     6241 gtacccgttc cggcacgctg aaactggaag aggacgaaac agggttacgc tttgaactga
     6301 ccccaccgga taccagcaca gggcgggacg ttatcgaact ggttaaacgt ggtgatatat
     6361 ccggcatgag ctttggcttt cgttcccgta aggatgtatg ggataccaca acagaaccat
     6421 gcgtgcgcac cgtgctggtg gcggaactgt acgaaattac cgttacatcc gttccggctt
     6481 accctgattc cggcgtggag ctggcccgcc gttccctgta tgagcagcac cccgaaaaaa
     6541 tgccgcgtgc ggataatcgc cgctggtggg tggatttagc gggggtgtga tatgtggcct
     6601 ttcagaagaa aaaaagagca gcgcagcatg acgcttgatg aatttatggc gctggctggc
     6661 acatcgaaca cgggggcggg tgagtacgta tcatcgggga cagcggaatc actgcccgcc
     6721 gtcatgaacg ccgtcacggt catctctgag gcggtggcta ccatgccgtg ttacctgtac
     6781 ctggtacgca atgagaaggg gaaggaggcc cgcgagtggc ttgattctca tccggtcgat
     6841 cacatcctca acgagcgccc gaacgcatgg caaaccccct accagtttaa gcgaatgatg
     6901 atccgccact gcctgttaaa cggtaatgct tatgcggtga ttcagtgggg gcgtgatggt
     6961 tttccggcgg ctttacatcc ttacccgccg cagtcggtga acgtggagca gacaggagaa
     7021 cacaactggc gctattgcat cactgacgcc tacaccggaa acacccgcaa ctatttaccg
     7081 tgggaagtac ttcaccttcg ttactccacg gatgacggct ttatggggcg ctcacctgta
     7141 accatctgcc gcgaatcgct ggggcttggg ctggcccaac aacgccacgg cgcgagcgtg
     7201 atgcgtgatg gcatgatggc ggcaggggtt atcacgtcag gcgaatggct ggacggcgtg
     7261 aaaggcaaac aggcattagc cgcactggaa cgctacaaag gggccagaaa cgccggaaaa
     7321 acgcccatcc ttgaaggggg catgagttac cagcagctgg gcatgagtaa ccaggacgcc
     7381 gaatggctgg cctcccgtcg cttcaccatt gaagacatcg cccgaatgtt caacgtctcg
     7441 ccgatttttc tgcaggaata cagcaacagc acctacagca atttcagcga ggcaagccgc
     7501 gcatttctca ccatgacgat gcgcccgtgg ctggcgaact ttgagcagca gataaaaaac
     7561 gccttgctgg tggtctcgcc tgtacctggt atccggtatc aggtggagtt tgacagcgcg
     7621 gacctgttac gggccacacc tggcgaacgc tttgccacct atgagcgagg catcaaatcc
     7681 ggtgttatgt gcccgaacga agcccgcgaa cgagaagggt tgtctccgcg tgatggtggt
     7741 gatgagttca gccaggcatg gaaacaggaa gtaaaaatca gcgagggaga aaaaccggaa
     7801 tgaacatagg gcgactgcgt gacagggtaa cgattcagac cctgaaacag accagagcca
     7861 tgacgggcga aatactcgaa acgtgggagg acggtcacac actctgggca agcgtgaaca
     7921 tggtcagcag caaggaggcc atttcatcgg gtgcagagct ggcgattggt accgtaagga
     7981 tctggatacg gtaccggaag gacatcaacg ccaccagccg gataaaggtc aatacggggc
     8041 cgctggcggg gcgtgtactg aatatcatcg ggcagccgct gccggatgcc gccaggacac
     8101 gccttgaaat tctttgtcgt gagggcgcgg aaaaatgaca gaagaactta tcaccctgga
     8161 agaagtgaaa ctccattgcc gcatcgatgg cgacgaggaa gaccagttaa tcagcggata
     8221 cattgccgca tcgcttgagg cgtgccagat acacataggc aggcgctttg atgacgggct
     8281 ggagttcacg ccagccataa agattggctg catgatgttt atcgctcacc tgtatgagaa
     8341 tcgccagatt gtcgcggata acgcaaaaac acacgtaccc atgacgattg gcgcactctg
     8401 gacggcttac cgtgatgtgg gggtgtactg atgccgtggc aaccattaag acgatgtaca
     8461 gaaccaggct gcaacaggcg cgtgaagtcc ggcaagtgtg aggagcacag acgcagcgca
     8521 cgccagcagc aggacagccg gagaggcagc agcagggagc gagggtatac gaggcagtgg
     8581 gagaaatacc gcgccatgta tctgagtaaa aacccgttat gtgcgcattg cctggaaaaa
     8641 ggaatataca cgcccgccgt ggtggtggat cacattatcc caatcgatgg cggtaatgat
     8701 gttctcttct ggcctgagtg gaatcatcag ccattgtgcc aggcgtgtca taaccagaaa
     8761 acgaaatggc ttgatccgtc cacaaaaaac aagcgtgctg cgggtggatt ccgtgaggag
     8821 gaagaacggg ccgctaaccg caataactgg atgtatggcg ctgatgaatg agcgggaaca
     8881 aaaccgcctt atccgtggac ttataaggca gcgtgacgca tggaaggcac aggaaacagg
     8941 gcataaagat aaagcatcag gacgcgcaga acgcatcaca gcgacgcgat taaacgaccg
     9001 tgaccgcgag gttatggaat gtttccgcaa tcgctgatga ggcagccgga cggggtgggg
     9061 ggagttttca ggacgaaccc gaccccgccc agaaccgaac gcctcctcaa atttttatgc
     9121 acgggaattt tttgaaaaat aatctggcga aaaataagca tgtcaagacc accgaaagcc
     9181 cccgcctacc tggatgatat cgccgtgaag cagtggcggg aaaaatctag gcagcttgcg
     9241 gaacggggag acctgacccc cgccgactgg agcaatctgg aactgtattg tgtcaactac
     9301 tccatttacc ggaaagccgt tgcagacctt gcggcgcgcg ggttcagcat tgttaacagt
     9361 cagggcggcg agagcagaaa ccccgcatta agcgcaaaat ccgacgctga aagagtgatg
     9421 ataaaaatgg cctccttgct cggttttgac ccgataagcc gccgtaaaaa tccaccggaa
     9481 acagaagaag aggacgagct tgaccgcctg gaataagtac gcagaagacg taaaaacggg
     9541 caaaattccg gcctgtaaac ggctgaaaca ggccgttaaa cggtactttt cggaccttga
     9601 aagccccctt tacacgttcg atcgtgaggt tgtggagcgg tttattgcct tttccagggt
     9661 gtgcccgcac gtaaaagggc cgatgcgtgg cagacccatt gagctggagc cgtggcagca
     9721 gttcgccttt gcgtgcatcc tcggctttaa ggttaaggcc accggacggc gcaaatacac
     9781 gagcgccttt atcgaagtgc cgcgcaaaaa tgcgaaatcc acgaccgccg cgattctggc
     9841 taactggttt ctgattatgg agaacgggca gcaggatatt tacaccgcag cggtgagccg
     9901 tgaccaggcg cggatcgttt ttgatgatgc gcgtcagatg tgccttttat cccgaccgtt
     9961 acgcaggcgg gtgaatattc aggcccacaa ggtgatgcac ccgaaaagca acagcctgtt
    10021 aaagccgctg gcagcaaaag cggcaaccat cgagggcaca aaccccagcc tttccatcgt
    10081 ggacgaatac cacctacacc ccgataacgg cgtttattcc gcgcttgagc tggggatggg
    10141 cgcacgtccc gaagggctgt tatttgccat taccacatcg ggcagtaacg tcgtttcagc
    10201 ctgtaagcag cactatgatt attgctgcca gatcctggac ggcgaagagg tcaacgattc
    10261 aatttttgta ctgatttacg agctggacga cgaaaacgag gttgatgatc cggcgatgtg
    10321 gataaaggct aaccctaaca tcgatgtttc cgtggatcgt gaaaaactgg cctcaaccat
    10381 ccagaaagcg cggggtattc cgtcgcaatg ggtggagatg atgacaaagc gatttaatat
    10441 ctggtgccag ggggctaccc cgtggatggg taacggcgca tgggctgagt gtgccggaac
    10501 gttcacggag gaagatttac acgggcagga gtgttacgcg gggctggact tatcatcaac
    10561 cagcgatatt tccagcgtgt gctatgcctt tccggtcggt aaaaatatca tgctggtttc
    10621 ccgtcattat ctgccggagt tccagctaca gaaccccgcc aataaaaacc gcgctgtcta
    10681 tcgccagtgg gctaaagcgg gctggatacg cacaacgcca ggtgactgca ttgattacga
    10741 ccggatcaga gatgacatca tgcaggatgc ggagaaattt aatatcaggc tggtgggctt
    10801 tgatacgtgg aacgccacgc atctcaggac gcaattacag ggggcaggct ttgaggtgga
    10861 gccgttcccg caaacctacc tcagattcag tccggcggcg aaatcgttcg aagtttttgt
    10921 taaccgcagg gtgattgtgc atcgtggcga tccggtgttg tcctggtcga tgagtaacgt
    10981 cgtgatgcag agtgacgcga acgccaatat caagccgaac aagaaaaaat caccgaacaa
    11041 gatagacccg agcgtagctg cgctgatggc gtttggtaca ttccaggcag agcatgagga
    11101 ttttgctttt gatataagcg acagccaccg ccagaaactg gaagaattta gcggggtgta
    11161 atgaggtcag cagcatgaca gaagccgaaa tactgggatt aatccgccgc gtctctggaa
    11221 tcagccagca gcatgacgaa cagggcacgc aaccggacag cgtgaccgct gaaaattatg
    11281 cgcgtgttgt tgctgaggtg atgcgccgtg atggtattga gcttaacggc gtggatatgc
    11341 gcaacatacg aaccagagtc cttgagttgc tggcataccg tcgccgcgtg gagatgtatc
    11401 gggagaagga gaaaatcacg taccactgga agaagccgga gcggttacgg cggtaactca
    11461 ctgagattaa agcaaagcgc gaaattgcgc tggctgaatg atcattatga ttacttatgt
    11521 ttacgcagat gattaaggcc taaaccgttg attattcaca atcccccatt gggggaagct
    11581 ggcggtaact ggttgaactt aaagcaaagc gcaaaattgc gctggctgaa taatcattat
    11641 gattacgcag atgattaagg aatgaccgaa ggcggaaatt cgcctgtggt tgatgggggg
    11701 agttgcagat ctgcaactcg accatgaaat tacggaaact acccgtagtt tgggtagtaa
    11761 gagcaacacc cagattttgg ggcttactcg tgatacccaa atttggggta tcggcggcaa
    11821 ccataacgac tttcgttacg gttgatgctt ttaccccatt gagggaaagg tattacgata
    11881 atcataacac ctaccgaaca ggtaaagccc accagcctga ttaactgtta accggaaaaa
    11941 aattccggta gggttcccga tatgggaatc cccatatcga cagcaacgcc cccttaaaat
    12001 tacccgcctt tatatttctt gtgcctattt gttccacgtt gtttcataca gtgcgccgaa
    12061 cggtgtagtc actggtgtag ttattttgag tttttttgca cttcgggctt aa
//
LOCUS       DAZFRN010000142        12073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFRN010000142 DAZFRN010000000
VERSION     DAZFRN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.144"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            210..980
                     /gene="yjjP"
                     /locus_tag="ACHKUB_003912"
     CDS             210..980
                     /gene="yjjP"
                     /locus_tag="ACHKUB_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO6656871.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            971..1444
                     /gene="yjjB"
                     /locus_tag="ACHKUB_003913"
     CDS             971..1444
                     /gene="yjjB"
                     /locus_tag="ACHKUB_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO6656872.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSESLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1552..2091
                     /gene="dnaT"
                     /locus_tag="ACHKUB_003914"
     CDS             1552..2091
                     /gene="dnaT"
                     /locus_tag="ACHKUB_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO6656873.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2094..2831
                     /gene="dnaC"
                     /locus_tag="ACHKUB_003915"
     CDS             2094..2831
                     /gene="dnaC"
                     /locus_tag="ACHKUB_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO6656874.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2880..3374
                     /gene="yjjA"
                     /locus_tag="ACHKUB_003916"
     CDS             2880..3374
                     /gene="yjjA"
                     /locus_tag="ACHKUB_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO6656875.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3628..5919
                     /gene="opgB"
                     /locus_tag="ACHKUB_003917"
     CDS             3628..5919
                     /gene="opgB"
                     /locus_tag="ACHKUB_003917"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO6656876.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6059..7081)
                     /gene="lgoD"
                     /locus_tag="ACHKUB_003918"
     CDS             complement(6059..7081)
                     /gene="lgoD"
                     /locus_tag="ACHKUB_003918"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO6656877.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7220..8134
                     /gene="lgoR"
                     /locus_tag="ACHKUB_003919"
     CDS             7220..8134
                     /gene="lgoR"
                     /locus_tag="ACHKUB_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO6656878.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8349..9710
                     /gene="lgoT"
                     /locus_tag="ACHKUB_003920"
     CDS             8349..9710
                     /gene="lgoT"
                     /locus_tag="ACHKUB_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6656879.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9759..11423)
                     /gene="tsr"
                     /locus_tag="ACHKUB_003921"
     CDS             complement(9759..11423)
                     /gene="tsr"
                     /locus_tag="ACHKUB_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO6656880.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11542..11988)
                     /gene="hpaR"
                     /locus_tag="ACHKUB_003922"
     CDS             complement(11542..11988)
                     /gene="hpaR"
                     /locus_tag="ACHKUB_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO6656881.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2928 a         3190 c         2937 g         3018 t
ORIGIN      
        1 acaataaatt cattatgatg ataatattca tgtgatagtt tgtttgcggc gagagataat
       61 tcgcttttta tcaccgagct tctcccgata accaggatta atcagcgcaa ttttgatctt
      121 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      181 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacgc
      241 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      301 atgagctttc ctcacgactg ggtcgggcgc tgggaatgga cagcgttgaa agttctatct
      361 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      421 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      481 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
      541 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
      601 gtaaactcaa taacggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
      661 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccacagatc aacttttgcc
      721 ttaccgcttt cgccgccacc accatttccg gattgctttt gcaactcccc actttcagca
      781 atacccccac cattgcgatg gccgccagcg ttctgctgct ggtgccgggc tttccgttga
      841 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
      901 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
      961 ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata tgatcctcgc
     1021 cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cattacgctg
     1081 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1141 gaatattgag tggtcaacct ttatggcttc tatgctggtc ggcaccattg gtattcaatg
     1201 gtcgcgctgg tatctggcac atccgaaagt gtttaccgtg gcggctgtta tccctatgtt
     1261 ccctggcata tcggcttata ccgcaatgat ttcggccgta aaaatcagcc agttaggtta
     1321 cagcgagtca ttgatgatta ccctgttaac caattttctt acagcttcat cgattgttgg
     1381 tgcgttatcc atcggtcttt ccatccctgg attatggttg taccgcaagc gccctcgcgt
     1441 ataaaattgc ctcgcttaaa gcacgcggat gagagacagc cacctctcct ccgtgtgtta
     1501 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     1561 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     1621 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     1681 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     1741 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagctgc tcccgttgct
     1801 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ctttatccgc
     1861 ctggcggcgc tatggggcgt ggcactaaga gagccggtga ccaccgaaga actgacctca
     1921 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtacagtg gcaacaaaaa
     1981 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2041 aatacggtca gtgaacctga cagccaaatt ccaccaggat tcagagggta acgatgaaaa
     2101 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacccgcat
     2161 tcaaaacggg tgaagaacta ctggcctggc aaaaagaaca aggggcgatc cgctccgccg
     2221 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2281 gtccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2341 atgcgttaag caaagcgcgc cagtatgtcg aagagtttga cggcaacatc gccagcttta
     2401 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2461 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     2521 aagatacctt caggaatagc ggtaccagtg aagaacaact gcttaacgat ctgagcaacg
     2581 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     2641 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     2701 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     2761 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta
     2821 aagagtatta atccccgttt ctggcggtat actccgacac aattccacct tgagtaatga
     2881 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     2941 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3001 accaaaacag tacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3061 gcagcggaaa ccaggcctta agcgcagata acatgaacaa tgccgcaggc attctgcaat
     3121 actgcgcgaa gcaaaagctg gcttcggtaa ccgatgccga aaacatcaag aaccagatgc
     3181 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggacggca
     3241 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3301 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3361 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3421 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3481 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     3541 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     3601 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     3661 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     3721 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     3781 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     3841 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     3901 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     3961 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4021 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4081 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4141 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4201 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4261 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4321 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4381 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4441 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4501 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     4561 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     4621 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     4681 gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc
     4741 gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     4801 tcttctgacc atttagcgat gaacaacacg gcgtggaaat accttaataa gcaggatcgc
     4861 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     4921 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     4981 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5041 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5101 aaagagttca ctatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5161 ttgccgctgc tgttgcgggt ttcagacaag cgcgtagaac cgctgccgga aagcgaatac
     5221 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5281 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg
     5341 tgtgtctcgc aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca
     5401 tggcagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat
     5461 gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc
     5521 aacgtcgccg gtgcgcctga agaggtgaaa cagtttagtg ggatttcccg tccagagtcg
     5581 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     5641 ctgccgaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     5701 cctattccgg tacgcgtagg caatgaagaa caaactcttg tgctgggcaa tgaagtgacc
     5761 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     5821 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     5881 ggcatggtgg aaattaaagt tgtggaacgt gaagggtaat tcctgactga tgtgtaggtc
     5941 gcataaggtg cgacctacac accttctccg gcctacaaaa catcattgag aaacagaaaa
     6001 aaaacagcgc ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc
     6061 agaaagtaat tacaccctta atcaactcac gattgttaat cacatcgcgc tcgtaggttt
     6121 ctgccagcgt ggcgaacgga tagcgatggg ttaacatcat gtcggcagtg atttttcctt
     6181 ccgccatcag tcgaccgact ttagcaaaat cttccggcgt ggcgttgcgg ctgcccatca
     6241 tcgtcgtttc ttttttatgg aattccggat cggagaactg caactcacct ttaaacaggc
     6301 cgacaaatac caccgtgccg ccgtgacgaa tcaaattcac ggtgttattc atcgcatgtt
     6361 gattacctgt cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgtagct
     6421 gcgcgtcaaa atcttcggct gacgggtcca gtaaaggtaa ttccagacgc gttgccacat
     6481 gttcacggcg cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga
     6541 ttgccgccgc gcccagaccg attggccccg ccccgaccac cagcacctgc tcgccgggag
     6601 caatggctgc gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgcggccg
     6661 cctgcgggtc aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac
     6721 taaaaccgcc atcctgatgc acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc
     6781 cgcttttaca cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac
     6841 catttttaag atcagcaata tttttaccca gcccaacaat ctccccacat atttcatggc
     6901 ctaaaacacg tggataacta aaaaatggtt gatttccacc ccaggcatga atatcggtac
     6961 cgcaaatccc gacagactta atttttatta atgcttcatt gtcacccgga ataggtatct
     7021 cgcgttgttt ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca
     7081 tgattcggct ctccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg
     7141 aacggaaaat ctgttttgtg aaaacacacg cataaaaatg ttttaaatcg ggtttcaatg
     7201 gactcacaag gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacca
     7261 ggtcattgat gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct
     7321 ggcggaaatg tacaacatca gccgcacaac agtgcgtcat attctcagcc acttacgcga
     7381 atgcggcgtc ttaacgcagg ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga
     7441 tgacggtttc gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc
     7501 ttttttcacg atgataaacc agcgtcagtt acgccctggg gaaacttttt ctgaactgca
     7561 actggctcgg gccgcgggtg tcagcccggt ggttgtaaga gaatatcttt taaaattcgg
     7621 ccgttataat cttattcaaa gcgaaaaacg cggccagtgg agcatgaagc aattcgatca
     7681 atcctacgcc gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca
     7741 ctttctcaac ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctcgaacg
     7801 tcatcggcta ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag
     7861 agattttcac tccttactgc tttcagctgc cgacaatatc ttttttgatc aatcgcttga
     7921 gatcatctca gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg
     7981 caatatcatt gccgtcgatg agcatatgac cattctcagc gctctgattt gccgcagtga
     8041 ccttgatgcc acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg
     8101 atccattaat gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt
     8161 agcgggtgca cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca
     8221 caatcagtcg actaaaaatt tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc
     8281 cgttaatgtc aaccttgcgt aattcacaat tacgtaaaca attaaaatta taaatacagg
     8341 agtccggcgt ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt
     8401 cgtcggcaga tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac
     8461 gcattcaaac caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc
     8521 gcagttcgct gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg
     8581 aaatcggcgc tttgctctcc gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg
     8641 gcccactatt ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt
     8701 cactgttcca ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta
     8761 tcggtatggg gattggtgaa gcgccgatga acccatgtgg cgtaaaagtc attaacgact
     8821 ggttcaacat caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg
     8881 gcgttgccgt aagcccaccg attctggcgg cgatgatgct ggtgatgggc tggcgtggga
     8941 tgtttattac cattggtgtg ctggggattt ttctcgccat tggctggtat atgctctatc
     9001 gcaaccgcga gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg
     9061 tcaatgcccg ccgagatccg ctcagttttg ccgaatggcg cagcctgttc cgtaaccgca
     9121 caatgtgggg aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg
     9181 cctggcttcc tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga
     9241 tggcggctat ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg
     9301 actggctggt caaaggggga atggctccga ttaaaagccg taagatctgc attattgccg
     9361 ggatgttctg ttctgccgcc tttacgctgg tcgtaccgca agcgacaaca tccatgacag
     9421 cggttctgct gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg
     9481 gcttgatcca cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact
     9541 ttgccagctt catctgcgcc tcttttgcgc cgatcattac tggttttatt gttgatacca
     9601 cccactcatt ccgtctggca ctaatcatct gcggttgcgt caccgcagcg ggggcactgg
     9661 cgtacatctt cctggttcgt cagccgatca acgacccacg taaagattaa tctccctatg
     9721 cccgataaca ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc
     9781 ccgctatctg ccacggccat tttacgcggc gtagctggcg tcacggtttt taccacagcc
     9841 gatgtttcac gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc
     9901 aggcgactgg cctgctcttc cagcgcggcg gcggcggcgg cagattcttc caccagcgcg
     9961 gcgttctgtt gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca
    10021 cggctctgct catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca
    10081 gcactgacaa tctccgccat tgtttccccg gcgctttcga ccagtgtaga gccaacatcc
    10141 actttcccca ccgagtcttc aataaggctt ttaatttcac gagccgcctg agcgctacgc
    10201 tgggccagat tacgcacttc tcccgcaacc accgcaaaac cgcgcccttg ctcacccgca
    10261 cgcgctgctt caaccgccgc gttcaaagcc agaatattgg tctggaaggc aatgccgtca
    10321 attacgctga taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc
    10381 accacgttat ctaccacttt gccgccccgt tgcgccgttt cagaagcact taatgccaga
    10441 tggctggcct ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg
    10501 ctggctgccg tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta
    10561 ttgccggtag cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca
    10621 ccgacggtac gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc
    10681 tcattagagc catccacctc aatcggtttc accagatcgc cgcctgcaat atgacggatg
    10741 ctgtcaatca ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg
    10801 aagatgaccg ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag
    10861 gaggcattgt tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc
    10921 acatactgct tctcgaaacc gtcttgatat ccctgggtcg gctgatcaaa gaactcgttg
    10981 attttgcctg cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa
    11041 ttacgtttga tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg
    11101 taatccgccc agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca
    11161 gcaacggttg aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg
    11221 ttgagggtgt tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc
    11281 tggcgaatgg tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaagaac
    11341 agaccgcctg atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc
    11401 acgatcttga tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg
    11461 cctggaaagc aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc
    11521 tggttatcag atactaaaaa gttattcatt atcgcccggg atggcttctt gtcgggaatt
    11581 gcccagagca ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa
    11641 ctgagcttca atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc
    11701 ctgcccctct ttggttagcg agatatagag cttccgctga tcattgatcg gctttaatcg
    11761 caacactaaa ccgtcgcgtt ccatccgcgt aaggatcccg gtcaggcttg ggcgcaaaat
    11821 gcaggcgcga tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac
    11881 gatgcgccac tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat
    11941 cgccgcttct cgcgcctgga gcaacgcaat ggttagagag tcgtgcatta tctttcccct
    12001 gaaaaattca aaatcattaa tatagaaaca gttaatcatc acgttaccgg gactgcgctg
    12061 cccctgcaat tct
//
LOCUS       DAZFRN010000143        12013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFRN010000143 DAZFRN010000000
VERSION     DAZFRN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.145"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            157..720
                     /locus_tag="ACHKUB_003923"
     CDS             157..720
                     /locus_tag="ACHKUB_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656882.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            802..3444
                     /gene="yfcU"
                     /locus_tag="ACHKUB_003924"
     CDS             802..3444
                     /gene="yfcU"
                     /locus_tag="ACHKUB_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO6656883.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3464..4216
                     /locus_tag="ACHKUB_003925"
     CDS             3464..4216
                     /locus_tag="ACHKUB_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6656884.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4191..4739
                     /locus_tag="ACHKUB_003926"
     CDS             4191..4739
                     /locus_tag="ACHKUB_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656885.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLNSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4736..5206
                     /locus_tag="ACHKUB_003927"
     CDS             4736..5206
                     /locus_tag="ACHKUB_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656886.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5203..5727
                     /locus_tag="ACHKUB_003928"
     CDS             5203..5727
                     /locus_tag="ACHKUB_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6656887.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTSFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSAIVTATFEYF"
     gene            5714..6586
                     /locus_tag="ACHKUB_003929"
     CDS             5714..6586
                     /locus_tag="ACHKUB_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO6656888.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYNSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6708..7259)
                     /gene="smrB"
                     /locus_tag="ACHKUB_003930"
     CDS             complement(6708..7259)
                     /gene="smrB"
                     /locus_tag="ACHKUB_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO6656889.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7425..8357
                     /gene="prmB"
                     /locus_tag="ACHKUB_003931"
     CDS             7425..8357
                     /gene="prmB"
                     /locus_tag="ACHKUB_003931"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6656890.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8392..9477
                     /gene="aroC"
                     /locus_tag="ACHKUB_003932"
     CDS             8392..9477
                     /gene="aroC"
                     /locus_tag="ACHKUB_003932"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO6656891.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9481..10305
                     /gene="mepA"
                     /locus_tag="ACHKUB_003933"
     CDS             9481..10305
                     /gene="mepA"
                     /locus_tag="ACHKUB_003933"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO6656892.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10305..11114
                     /locus_tag="ACHKUB_003934"
     CDS             10305..11114
                     /locus_tag="ACHKUB_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO6656893.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11114..11662
                     /gene="epmC"
                     /locus_tag="ACHKUB_003935"
     CDS             11114..11662
                     /gene="epmC"
                     /locus_tag="ACHKUB_003935"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO6656894.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11696..11974
                     /locus_tag="ACHKUB_003936"
     CDS             11696..11974
                     /locus_tag="ACHKUB_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO6656895.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
BASE COUNT         2986 a         2914 c         3120 g         2993 t
ORIGIN      
        1 actatgcggc cccacaccaa cgaatacaat tttctacgac ctgttctttt tggatcgcat
       61 gccgtattca atggctgggt ataaacattg gctattgact gaatcaatgt attgcaatgt
      121 ttatattttt aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct
      181 attgctgcgg caatggtgat gggcgtgttc acctctacgg caacaatcgc tgcgggtaac
      241 aacggtactg cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac
      301 gatcacaaac tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc
      361 accaccacgc cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tactcaggaa
      421 acaatgacca ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc
      481 attttcaata ccgataccgg tgcggcattt aacaatgtta gcctggcgat cggtgactct
      541 ctgggtacct cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct
      601 accaataaag gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct
      661 gatgcgccag atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa
      721 ggcatacccc catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa
      781 taaaaacgga tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg
      841 tggtgcattg cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt
      901 cagtttgatt cccgttttct ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt
      961 tccagccagg gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag
     1021 ccattggcgg aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc
     1081 tatgcttgtc tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaaa
     1141 aatctgcaat ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa
     1201 attaaggctg atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa
     1261 tatacttatc ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc
     1321 gcggactaca gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac
     1381 gagatcagcg gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac
     1441 tggcagacta actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa
     1501 actcaaaaaa aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa
     1561 gccaaactgg cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat
     1621 gttggtggca gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg
     1681 ccagacattt ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt
     1741 gtgatttacg aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct
     1801 gtctccggta cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac
     1861 atcagcaccg cctcgatgcc atacctcact cgtccaggtc aggttcgcta taagatcatg
     1921 atgggccgtc cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa
     1981 gcttcctggg ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac
     2041 tatcagtctg cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt
     2101 gatgttactc actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg
     2161 gacggtaact ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt
     2221 acttttgctg gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat
     2281 gccagtgaca gcggaatggt acgcacgggc aacgacaaag agatgtacac cgccacttat
     2341 aaccagaact tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac
     2401 tgggatcgcg aggagcagac aaactacaac atcatgctct cgcactattt caatatgggc
     2461 agtattcgta atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc
     2521 gacaaaggca tgtacatttc gctcagtatg ccgtggggcg acaacagtac cgttagctat
     2581 aacggtaact atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat
     2641 gacgcgactc actatcagtt gaacgtcggc accagtgaca aacacaccag cgttgacggc
     2701 tattacagcc atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg
     2761 caatacacct ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc
     2821 gcacttcacc gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt
     2881 gccgatgttc cggtagaagg taacggtgct gctgtttata ccaatatgtt tggtaaagcc
     2941 gtcgtttctg acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacaaactg
     3001 ccggaaaacg ccgaagcaac ccagtcggtg gtacaagcca cgctaactga aggggcgatt
     3061 ggctaccgca aatttgccgt catcagtggt caaaaagcga tggcggtgct gcgtttacaa
     3121 gacggcagcc atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc
     3181 cttgtcgatg atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt
     3241 gtgttctgga gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat
     3301 ctgtttaacg gcttgttact gccatgccag cataaaggca acgtcgcgcc ggttgttcct
     3361 gatgacataa agcctgtcat tcaggagcag acgcaacagg tgacacccac caatccccct
     3421 gtttctgttt cagctaacca ataacgtgat taaggaatga tccatgtctg atttactttg
     3481 ctctgccaaa ttgggtgcta cgacgctagc gttactgctt agcgctgcca gtttgtctgc
     3541 ccaggcttct gtaacgccag atcgtacccg tttgatattt aatgaaagcg acaaatcaat
     3601 cagcgtgacg ttgcgcaata acgatccgaa actgccttat ctggcccaaa gctggatcga
     3661 agatgaaaag ggtaacaaga tcagttcacc gttaactgtt ctgccaccgg tgcaacgcat
     3721 cgattcaatg atgaacgggc aggtaaaagt gcagggaatg ccggatatca acaaattgcc
     3781 tgccgatcgt gaaagcctgt tctacttcaa cgtgcgtgag atcccgccaa aatcaaacaa
     3841 agccaacacg ctgcaaatag cactacaaac gcgtattaag ttgttctggc gaccgaaggc
     3901 gctggagaac gtcagcatga aaaacccctg gcaatataag gttactttaa cccgcaacgg
     3961 tcaggaattt accgtcaata acccgacgcc gtattacgtc attttcagta atgccagcac
     4021 acagaaaaat ggcaatccgg cagccggttt ctcgccaata gtgatgtcgc cgaaaacctc
     4081 tgcaccgctg aatgtgaaga tgggtagcgt gccggtactg acctatgtca acgactatgg
     4141 cgctcggatg ccgctctttt tcacttgcaa cggtaacagt tgccaggtcg atgaagagca
     4201 gagccgcaaa ggctgaggcc gggagagcca gtatgaaagg atcttgttct gtctgggcgg
     4261 taactgggtt actgatatgt tgtgttggta gcggctttgc agttgcagcg agtaagtcag
     4321 taagtatgaa tttacgggtg cttgtcgatg cccctcctcc ttgtacggta aatggtgcgg
     4381 cagttgagtt tggtaatgta ttcattaata agatcaatgg ggttgattat aaacgtccca
     4441 ttgactactc gcttgtttgt aacaatctgg caatggatga cctgcgtcta cagatgcagg
     4501 cgacaacggt cgtgattaac ggtgaaacag ttatcagcac gggtattcct gggtttggta
     4561 ttcgggttca gaaaagtagt gatcacacaa tactcgactt aaactctggc tcatggttgc
     4621 catttaattt ttcctcggga gttcccgttc tggaagctgt gccggtgaaa cagagtggta
     4681 caacgttagc ggcagctgaa tttaacgcca gtgccacaat tgtggtggat taccaatgaa
     4741 acggatctcg cttattttgc tgtggggctt ttgctcgatg gcactctcaa acgtctcttt
     4801 tcacggttat ttagtgcagc cgcctaactg cacgattagc aacgctcaaa ccattgaaat
     4861 aacttttcag gatgtgctta ttgatgacat taatggcagc aattatgagc aaaccgtgcc
     4921 gtattccatt acctgcgata cggcggttcg cgacccatta atggaaatga ccttgagctg
     4981 gagtggcaca ccaagcgatt ttgataatgc tgcagtaagc agcaatatca ctggtttagg
     5041 cattcagctc aaacaggcag ggcagagttt tacgatcaat acgccacttg tggtgaatga
     5101 aaccgattta ccggtgttaa cggctgtgcc agtaaaaaag agtggtgtcg ttttgccaga
     5161 ggcggacttt gaggcctggg ccacgttaca ggtagattac caatgaagaa aaagcgtacg
     5221 ttgtttttta ttagtagcct gatgttgctg ggcagtggaa ccacaatagc aggagataat
     5281 ttgcacttta ccggcaattt aatcagtaaa tcgtgtacgc cagtgattaa tggtagccag
     5341 ttggcagaag tacattttcc tgccattgcc gccagcgatt taatgaacct tgggcagtca
     5401 gagcgagtgc cattggtgtt tcaattgaaa gattgtcata gttctacatc gtttaatgtc
     5461 aaagtaacgt tgacaggaac agaagatagt gctttacccg gctttctggc ctttgactcc
     5521 tcgtcatcag catccggcgc tgggattggt attgaaactg cggcaggcac atcggtgcca
     5581 atcaacaata caacaggcgt cacgctccca ctgaatcagg gaaataacag cctaaatttt
     5641 aatacctggt tacaggcaaa gagcgggcga gatgtaacgt ccggagactt tagtgccata
     5701 gtgacggcaa cctttgagta tttctgaaat gaaaaaatgg accataattt tcgcgagcct
     5761 aatgctgctc gttctgagtg ttgtcggcgc atctaaaagt tatgctggag ataaacttat
     5821 gagtgcgagt tttgacagtg tgagagttta ttatgccatg gacaaggtaa ccggtgctgt
     5881 agcgagcagt gttttcaatg ttacggtgat aacgccaaag gaggttgcat atgggaaata
     5941 taattctttt gcgtataaag gcgatacgtt gcgggttatt tcctggtctg ggtcgggttc
     6001 agctccaact ttggtactga ctgactttga tacaattaat aactcaaatt gccctggcat
     6061 agatacgaaa atttttcgtt gtgcatatat gacgtttaaa attaccgttg catctgatga
     6121 ttatggttgc ccttggatcg catcctttta ttcctataca gatttacccg gatttggaag
     6181 ctatacggcc cctacagtgc ataacacaat atgcccaacg ataccggtcg cttcatacga
     6241 tatctcctgg agtgaaaatt atgtcagcca taataaggcg ttacgaattc agtccacggg
     6301 gtcaacagtg accaccacgc tttcgaccta cctgatggaa ggcggcagac tctgtgatgg
     6361 cagtaatttt tctgataatg atggcagagg agcatactgc cgcgcggtat cagaattgtt
     6421 aacctttacc tcttacggtt gtgataaatc aacggtaacg gttacgccaa ctcgtcatcc
     6481 ggtaacggat aaagtacttc acgatattgt ggtgaatgtg aataccagta gtgggcaacc
     6541 gattgattcg acgtgtcgtt ttcagtatgt attgaatgaa ctgtagttcg tagtattgct
     6601 ctgatgcgtc ttatcaggcc tacgaagtga attgcaattt attgaattat cacatatttg
     6661 taggccggat aaggcgttta cgctgctccg gcatttgctg ccagcattca gggcaactcc
     6721 ggcggcaacc actcttccac ttcaatcaac accaacaaag ccgcatcacc gccgtactct
     6781 ttcggtgcct ggtgaaatgc catcacatgc ggatgttgtg ccagccacag cggtgtttgc
     6841 tgcttcaata tgtgcttccc gtgaccatgc atcacgcagg cacaaaacac atgttcgcgg
     6901 cggcaggcag caatcaatgc gcctaattcc tgcttggctt gtagctgcgt cagaccgtgc
     6961 aaatccaaaa acaactccgg tgaataatcg ccgcggcgca gtttcttcgc ctcaaaatgg
     7021 ctgacatccg ggcgaacata tttcaccgga ccttcggtat tcaataacgg ctgaaactca
     7081 tcggaaaaat aatggctggc atcggcctgc tcctgaatca agcgtttcac cggcacttcg
     7141 ctaattttct tacgctgcgg tcggtggaca atcgtgtcct gcttaatctt gcgagtcccc
     7201 gccatcaact ggcgaaacag agcctggtcc tcctcgctaa gtgttgtttt ctttttcatt
     7261 tatctgtctc aatttctaat tttcctacag tttacccgac tcgtagcgca atcgatcgtt
     7321 ttaaacgtat ttttcctcac tcaccgcgcg tgaatgctga tttatcgtca tcttcatggc
     7381 aaactagccg ccgaaattat tgcgatcatg ccctggagga atacgtggat aaaattttcg
     7441 ttgatgaagc agtaaatgag ctgcaaacca ttcaggacat gttgcgctgg tcggtaagcc
     7501 gcttcagcgc ggcaaatatc tggtacggtc acggcaccga taacccgtgg gatgaagccg
     7561 tacagctggt gttaccatcg ctctacctgc cgctggatat cccggaagat atgcgcaccg
     7621 cgcgtctgac ctccagcgaa aaacaccgta ttgttgaacg cgtgatccgc cgcgtcaacg
     7681 agcgcattcc ggtggcttac ctgaccaaca aagcctggtt ctgcggtcat gaattttacg
     7741 tcgatgaacg cgtgctggtg ccgcgctcac cgattggtga actgatcaac aataaatttg
     7801 ccggactcat cagcaagcaa ccgcagcata ttttagatat gtgtaccggt agcggctgca
     7861 tcgccattgc ctgtgcttat gccttcccgg aagcggaagt cgacgcggta gatatctctc
     7921 ccgacgcgct ggcggttgcc gaacagaaca tcgaagaaca cggtctgatc cacaacgtca
     7981 ttccgattcg ttccgatctg ttccgtgatt tgccgaaagt gcaatacgat ctgattgtca
     8041 ctaacccgcc gtatgtcgac gcggaagata tgtccgacct gccaaacgaa taccgtcacg
     8101 agccggaact gggcctggca tctggcaccg acggcctgaa actgacgcgt cgcattctcg
     8161 gtaacgcggc agattacctt gcggatgatg gcgtgttgat ttgtgaagtg ggcaacagca
     8221 tggtacatct tatggaacaa tatcccgatg ttccgttcac ctggctggag tttgataacg
     8281 gcggcgatgg agtgtttatg ctcaccaaag agcagcttat tgccgcacga gaacatttcg
     8341 cgatttataa agattaagta aacacgcaaa cacaacaata acggagccgt gatggctgga
     8401 aacacaattg gacaactctt tcgcgtaacc accttcggcg aatcgcacgg gctggcgctc
     8461 ggctgcatcg tcgatggtgt tccgccaggc attccgctga cggaagcgga cctgcaacat
     8521 gaccttgatc gtcgtcgccc tgggacatcg cgctatacca cccagcgccg cgagccggat
     8581 caggtcaaaa tactctccgg tgtttttgaa ggcgttacta ctggcaccag cattggcttg
     8641 ttgatcgaaa ataccgacca gcgttctcag gattacagcg caattaaaga cgtcttccgt
     8701 ccaggccatg ctgattacac ctacgaacaa aaatacggtc tgcgcgatta tcgcggcggt
     8761 ggacgttctt ccgcccgcga aaccgccatg cgcgtggcgg caggggcgat tgccaaaaaa
     8821 tatctcgccg agaaatttgg tattgaaatc cgcggctgcc tgacccagat gggcgacatt
     8881 ccgctggaaa tcaaagactg gtcgctggtt gaacaaaatc cgttcttctg cccggacccc
     8941 gacaaaatcg acgcgttaga tgaactgatg cgcgcgctga aaaaagaggg cgactccatc
     9001 ggcgcgaaag tcaccgttgt tgccagtggt gtccccgccg gacttggcga gccggtcttt
     9061 gaccgcctgg atgccgacat cgcccatgcg ctgatgagca tcaacgcggt gaaaggcgtg
     9121 gaaattggcg acggttttga cgtagtggcg ctgcgcggca gccagaaccg cgacgaaatc
     9181 accaaagacg gtttccagag caaccatgcg ggcggcattc tcggcggtat cagcagcggg
     9241 cagcaaatca ttgcccatat ggcgctgaaa ccgacctcca gcattaccgt gccggggcgc
     9301 actattaacc gctttggcga agaagttgag atgatcacca aaggccgtca cgatccctgt
     9361 gtcgggatcc gtgcagtgcc gatcgcagaa gcgatgctgg cgatcgtttt aatggatcac
     9421 ctgttacggc agcgggcgca aaatgccgat gtgaagactg atattccacg ctggtaaaaa
     9481 atgaacaaaa ccgcgattgc gctgctggct ctgcttgcca gtagcgctag cctggcagcg
     9541 acgccgtggc aaaaaataac ccaacctgtg ccgggtagcg cacaatcgat aggcagtttt
     9601 tctaatggct gtattgtcgg cgctgacacg ttgccgatac agtccgaaca ttatcaggtc
     9661 atgcgtaccg atcagcgtcg ctatttcggt cacccggatc tggtgatgtt tatccagcgt
     9721 ctgagtagcc aggtgagcaa tctgggcatg ggtacggtgc tgattggcga tatggggatg
     9781 cccgctggtg ggcgtttcaa cggcggtcat gccagccacc agaccggact ggatgtcgat
     9841 atctttctgc aactgccgaa aactcgctgg acctccgcgc agctcttgcg cccgcaagca
     9901 ctggacttag tttcccgcga cggtaaacac gttgttccga cgctgtggaa gccagaaatt
     9961 ttcagcttga tcaaactcgc cgcccaggac aaagacgtca cacgcatttt tgttaatccg
    10021 gcgattaaac aacaactttg ccttgatgcg ggcaccgatc gcgactggtt gcgcaaagtg
    10081 cgaccctggt tccagcatcg cgcgcatatg catgtacgat tacgttgtcc tgccgatagt
    10141 ctggagtgtg aagatcaacc tttaccgcca ccaggcgatg gttgcggggc agaactgcaa
    10201 agctggtttg cacctccaaa accgggaaca acaaagcctg agaagaagac accgcctccg
    10261 ttgccgcctt cctgccaggc gctactggat gagcacgtga tctaatggaa acgtttaata
    10321 gcctgtttat ggtttccccg ctgttgctgg gagttctctt ttttgtcgcc atgctggcgg
    10381 gatttatcga ctcgattgcc ggtggcggtg ggttactcac cattccggca ttgatggcag
    10441 cgggaatgtc tcccgctaac gcgttggcaa ccaataaact gcaagcctgc ggcggctcta
    10501 tttccgccac tatctacttt attcgccgca aagtggttag tttaagcgat cagaaactca
    10561 atattgccat gacctttgtc ggctcaatga gcggcgcact gttggtacag tacgttcagg
    10621 ctgatgtctt acggcagatt ttgcccattc tggtgatttg tatcggcctc tatttcttgc
    10681 tgatgccaaa gctgggtgaa gaagatcgcc agcgccggat gtacggttta cccttcgcgt
    10741 taattgccgg tggctgcgtc ggtttttatg acggattctt tggcccggct gccggatcgt
    10801 tttacgcgct ggccttcgtt acgctgtgcg gattcaacct cgccaaagcc acggctcacg
    10861 ccaaattact caacgcaacg tcaaacattg gcggtctgct gctgtttatt ctcggcggca
    10921 aagtgatttg ggcgacgggc tttgtgatgc tggtggggca gttcctcggc gcgcgcatgg
    10981 gttcacggct ggtgttgagc aaaggacaaa agctgatccg cccgatgatt gttatcgtct
    11041 cggcggtgat gagtgccaaa ttactttacg atagccacgg acaggagatc ctccactggt
    11101 tggggatgaa ctaatgaaca gtacacacca ctatgagcag ttgattgaaa tttttaatag
    11161 ctgctttgcc gatgaattta atacccgtct gattaaaggc gacgacgaac cgatctatct
    11221 tcctgctgat gcggaagtac cgtataaccg aattgtcttt gcccacggct tttatgccag
    11281 cgcaattcat gagatttcgc actggtgtat tgccggaaaa gcgcgccgtg agctggttga
    11341 tttcggctac tggtattgcc cggatgggcg tgatgcccaa acgcaaagcc agtttgaaga
    11401 tgttgaagtg aagccgcagg cactcgactg gttgttctgt gtggcggcgg gatatccgtt
    11461 taacgtcagt tgcgacaatc tggaaggcga cttcgaacca gatcgtgtgg tgtttcaacg
    11521 gcgggtacat gcgcaggtga tggattacct agcaaacggc attcctgaac gcccggcacg
    11581 cttcattaaa gcactacaaa attattatca cacgccggaa cttacggcgg aacagttccc
    11641 gtggccggaa gcgctcaact aaagccagcg ccacgtttta caatgaggaa agaagatgat
    11701 cgcggagttt gaatcacgca ttctggcatt aatcgacggt atggttgacc atgccagtga
    11761 tgatgagttg tttgccagtg ggtatttgcg tggccacctg acattagcca tcgcagaact
    11821 ggaaagtggt gatgaccact ccgctcaggc ggtgcatacg accgttagcc agagtctgga
    11881 aaaagccatt ggtgccggtg aattgtcgcc gcgtgaccag gcgctggtga ccgatatgtg
    11941 ggaaaacctg tttcagcagg cgtcacagca gtaagttgcg gcgtctggag caccatgcct
    12001 gatgcgacgc tta
//
LOCUS       DAZFRN010000144        11914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFRN010000144 DAZFRN010000000
VERSION     DAZFRN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.146"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(517..1443)
                     /locus_tag="ACHKUB_003937"
     CDS             complement(517..1443)
                     /locus_tag="ACHKUB_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6656896.1"
                     /translation="MRHVPRGSAQPPLSLSALDSNGKSELQRAREHYAEPEQAKRFEF
                     KAYSSDDVKWRLNTLFHFKCAYCETFFSASSPVDVEHYRPKSRVSEDAAHPGYWWLAM
                     DWENLLPSCIDCNRKRRQHIVNQSSNLISLLENSRRAATSGGKKDSFPLANRGVRMQP
                     ESRDHFEEQALLLNPCEHQPESFLHFVSIGAPSLSLVVPTGDRESPHGATSIHVYGLN
                     RLGLVQDRTRYLRRLEFLGDMMISLGELIDQVDRMALEEVQRDEIIRPLRLLLDKTGK
                     EMTQMARPDQPYSVMAETWIAQFFQQIANRGV"
     gene            complement(1440..2237)
                     /locus_tag="ACHKUB_003938"
     CDS             complement(1440..2237)
                     /locus_tag="ACHKUB_003938"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6656897.1"
                     /translation="MSIEGEFDVIKRSPERKVCSIITAINGIETHTPLNVASSGFRTV
                     LAMVCDIMQGLMDERVAPQFEGFENARGIVLIDEVEAHLHPRWKMQIMGGLRRALPGV
                     TFIVTTHDPLCLRGMYNGEVRVMQRILHNDLRSESRASQRVETLTELPDITKLRVDQL
                     LTSDFFQLDSTDEPRLDRLIAQVADLLAKREQGEDLTAEESEVIGTFECDIASAMPVG
                     TSEAHRLVQEAVAEYLQERRQATATKIQALQDGTRSRILNILREVMQ"
     gene            complement(2438..3730)
                     /locus_tag="ACHKUB_003939"
     CDS             complement(2438..3730)
                     /locus_tag="ACHKUB_003939"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6656898.1"
                     /translation="MQYVDRSRVLPPPSLVTDNIVKENLYDIGAFLALESQRRAQTRP
                     PRLASLSKVEGLKDSLYELFNGRCAFCECNHHSLSIQRFRPSSNATPMGKNADAPLYY
                     LWLAWAWQNFYLVCAQCNELMMRNFLVTGKRMPVPSSQEYQDYLRSESSLWPQWPPKE
                     ESLLLDPCQIKSFASHFDVRPQGEFNYLSRRGMATINILNLNDADLISRRAQRYSEYM
                     KRLSESIEARSIDLLSSVLNFSRQEFGGSWYLLLRRLASHIDGEKGKALSMGKIKSFF
                     QLMFQNPEQGSDHLYQAFEDLYALPRDERIEEMPSPPKGDLRRVQFQNFKSLEAVELE
                     LPAMPELENGKKPEARALLLLGENAAGKSTILEGIALALVSAEGRKRLNLRLRDFILS
                     PALLAGDPRLAPHMAEVVLTQDNDASLTLTLKKVGVSS"
     gene            complement(4154..5104)
                     /gene="cbl"
                     /locus_tag="ACHKUB_003940"
     CDS             complement(4154..5104)
                     /gene="cbl"
                     /locus_tag="ACHKUB_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO6656899.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(5206..6123)
                     /gene="nac"
                     /locus_tag="ACHKUB_003941"
     CDS             complement(5206..6123)
                     /gene="nac"
                     /locus_tag="ACHKUB_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO6656900.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMALLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            6450..6525
                     /locus_tag="ACHKUB_003942"
     tRNA            6450..6525
                     /locus_tag="ACHKUB_003942"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6483..6485,aa:Asn,seq:gtt)
     gene            complement(6581..7516)
                     /gene="ldtA"
                     /locus_tag="ACHKUB_003943"
     CDS             complement(6581..7516)
                     /gene="ldtA"
                     /locus_tag="ACHKUB_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6656901.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(7578..8657)
                     /gene="cobT"
                     /locus_tag="ACHKUB_003944"
     CDS             complement(7578..8657)
                     /gene="cobT"
                     /locus_tag="ACHKUB_003944"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO6656902.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKAGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(8669..9412)
                     /gene="cobS"
                     /locus_tag="ACHKUB_003945"
     CDS             complement(8669..9412)
                     /gene="cobS"
                     /locus_tag="ACHKUB_003945"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO6656903.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFQHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(9409..9954)
                     /gene="cobU"
                     /locus_tag="ACHKUB_003946"
     CDS             complement(9409..9954)
                     /gene="cobU"
                     /locus_tag="ACHKUB_003946"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO6656904.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(10088..10525)
                     /locus_tag="ACHKUB_003947"
     CDS             complement(10088..10525)
                     /locus_tag="ACHKUB_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO6656905.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCQTLTRGSLAIPHSHLLS"
     regulatory      complement(10743..10918)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(11007..>11914)
                     /gene="pocR"
                     /locus_tag="ACHKUB_003948"
     CDS             complement(11007..>11914)
                     /gene="pocR"
                     /locus_tag="ACHKUB_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFO6656906.1"
                     /translation="AATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFTPFC
                     QMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGFVLC
                     GQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVENCL
                     KKQLNFVVIKENKSRSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASHVYL
                     SPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYFCKV
                     FRQTYQVTPQVYRQQVSENAAMETV"
BASE COUNT         3221 a         3094 c         2548 g         3051 t
ORIGIN      
        1 gggtttcata acaaatggat taataaaatc ctacagccaa agtactgaag atcctgaatc
       61 atacatcata gatttccctg aggaacttct tgattaatgc taatctttaa caataagtca
      121 tagatacact tctttaataa aggaaaacca ctggcatctc aattgctggc ggtttttctt
      181 ctcctcaaca tttcactgat gacataatca cctcacataa cggtagagat ggcaaaattg
      241 gtggtcatta tgacggtatt ggcaaggcta tgttttttga tttagctgta atttacaatc
      301 agttaatgac aaaggcgatc ccaatcagac ggatcacttc ctttatacgg tattcaagaa
      361 tatattgttg ttactcatgt gacttatgaa aatattacta acatcgtggt gtgttaatca
      421 acggggagca ggttatcttc tggctaccca tatatttacg acaagtttga acggagatag
      481 ccagatttga tgtatatagt ttacgcggga actccttcat actccccggt tggctatttg
      541 ttgaaagaat tgggctatcc acgtttcagc catcactgaa tatggctgat ccggtctagc
      601 catctgagtc atttctttac cggttttatc caataataat ctcaatgggc gtatgatttc
      661 gtccctctga acttcttcta acgccattct atccacttga tcaataagct caccgagcga
      721 tatcatcata tcacccaaaa attcgagtcg acgtaagtaa cgcgtgcggt cctgaactaa
      781 accgagacga ttgagcccgt agacatgaat agacgttgcg ccatgtggac tttctctatc
      841 tcctgtaggc actaccagac taagagaagg tgcgcctatg ctgacaaaat gcaggaatga
      901 ttccggttgg tgttcgcagg gattaagtaa caaagcctgt tcttcaaagt gatctctgct
      961 ttcaggctgc atacgtaccc ccctattggc tagaggaaag ctgtcttttt tccctccaga
     1021 agtggctgct ctccggctat tctccaaaag agaaataaga ttactggact gattaacaat
     1081 gtgctgccgc cttttacgat tacagtcaat gcaactgggt aaaaggttct cccagtccat
     1141 cgctagccac cagtatcccg gatgcgcagc atcttcactc actctacttt ttgggcggta
     1201 gtgttcgacg tctaccggtg aagaagcaga gaaaaaagtc tcacaatagg cacacttaaa
     1261 atggaatagt gtgtttaatc gccattttac atcgtcggaa gaataggctt taaattcaaa
     1321 tcgctttgcc tgttctggct cagcataatg ttcccgcgct cgctgtaatt cactttttcc
     1381 attagaatcc aacgccgata atgataacgg cggttgggct gacccacgag ggacgtgtct
     1441 cattgcatca cctcacgcag aatgtttaaa atgcgtgagc gcgtcccatc ctgtaatgcc
     1501 tgaatttttg tagcggtagc ctgacgccgt tcctgtaaat attccgctac agcctcctga
     1561 accaagcgat gtgcctcaga agtccctaca ggcatagcac tggcaatgtc acattcaaat
     1621 gttccgataa cctcactctc ttctgcggta agatcctcac cttgttcgcg ttttgccagc
     1681 agatcggcaa cctgagcaat aaggcgatcg agcctgggtt catcagtgga gtcgagctga
     1741 aaaaaatcag aggtcagtag ttgatcgact cgtaatttag tgatgtctgg caattcagtc
     1801 aacgtttcaa ccctctggga agcccttgat tctgagcgta aatcattatg caatatacgt
     1861 tgcataaccc ggacttctcc attatacatg ccacgcagac atagcgggtc atgcgttgtt
     1921 acgataaagg tcacacccgg cagtgcccgt cgcaaaccac ccatgatctg cattttccag
     1981 cgtgggtgaa gatgtgcttc aacttcatca attagtacaa ttccccgagc attctcaaag
     2041 ccctcaaatt gcggggcaac acgctcatcc attaaaccct gcataatatc gcagaccatt
     2101 gccagcacag tgcggaagcc tgaagacgca acattcaagg gagtatgagt ttcgatacca
     2161 ttaatagcgg tgataataga acatactttt ctttcaggag aacgtttaat cacgtcgaac
     2221 tcgccttcaa tggacaacac ctccctaagc gctgcaacta ctcggtcaaa atctacgtta
     2281 ctgaggctca aaagccaact ttgtggattc ccgagtagac ttccatcgaa gagattgcgc
     2341 actgttttgt cagtagtata taaccgcctt ccttgtctat actgacgaaa agctccgtaa
     2401 gcaaatactg gcccaacatt aaactgccca ttcaaagtta cgacgatact cccacctttt
     2461 ttaacgtcag tgtcagcgag gcatcattat cctgagtaag tacaacctca gccatatgag
     2521 gcgccaaacg gggatcaccg gccagtaatg caggagacaa aataaaatcc cgtaaccgca
     2581 aattgagcct ctttcttcct tccgccgaga caagagctaa cgcaatacct tcgagaatgg
     2641 tacttttccc ggccgcattt tccccaagga gcagtagagc tctagcctct ggttttttgc
     2701 cattctccaa ctcaggcatt gcgggtagct caagttcaac cgcttccagc gatttgaaat
     2761 tctgaaattg tacgcgacgt aagtcacctt ttgggggaga tggcatttcc tcaatccgct
     2821 catccctggg aagcgcgtaa aggtcttcaa atgcctgata cagatgatcg cttccttgct
     2881 caggattctg aaacatgagc tggaagaaag attttatttt tcccattgac aacgccttgc
     2941 ctttctcacc atcgatatgt gacgcaagtc gtcttaaaag cagataccaa ctgccgccaa
     3001 actcctgtct actgaaattg agtacgctgg aaagcaaatc gatagagcga gcctcaatag
     3061 actctgatag gcgcttcata tactcactat agcgctgcgc acggcgtgat atcagatcag
     3121 cgtcattaag attcaaaata ttaatcgttg ccattcccct acggcttaag taattaaatt
     3181 ctccctgagg tctaacatca aaatgtgaag caaaggattt tatctggcat ggatctaaca
     3241 gcaggctttc ctcctttggc ggccactgag gccacaaact actttcacta cgcagatagt
     3301 cctgatattc ctgcgaggaa ggtactggca tacgtttccc tgttacaaga aaattgcgca
     3361 tcattaactc attacactga gcgcagacga gataaaagtt ttgccatgcc caagcaagcc
     3421 aaaggtaata aagcggtgca tctgcatttt ttcccatagg ggtagcatta ctgctaggac
     3481 gaaaacgttg aatacttaat gaatgatggt tgcactcaca aaaggcacac cgcccgttaa
     3541 agagttcata gagtgagtct ttaagcccct caactttgga taagctagcc agacgcggtg
     3601 ggcgagtctg agctctccgc tgagattcca acgccagaaa tgccccaata tcatagaggt
     3661 tttcctttac aatattgtca gtgactaaac ttggtggtgg gagcacacgg ctcctgtcaa
     3721 cgtattgcat ccgttcatgt ctccgtataa attactatct ctaactcaac agcgaaagtg
     3781 gggaaactga atcatactaa agggtatggc agacacacac tatttttccc tcgggtattc
     3841 cgggctaggg aagataaatg acatgaatca cagctcctga tgttgcgggt actaatttat
     3901 ttaatcatat actgactcat cacctcttcc gcccatctgg agcttgcgca tcgcccgcaa
     3961 accctgcctt gatctacttt ataaaagcgc catgcatatc ttgtatagaa tcataaaaat
     4021 tggtggtcat tttggtggtc ttgacaaaac cgcacttctg atttagctta cttattagcc
     4081 agttaatggc tacggcgatc ccagtcagag gagccaaatt tctgaaaagc ccgcttttat
     4141 agcgggcttt ttgctatatc tgataatcaa tttcctcttc actgttttcc atcacctgcc
     4201 gcttgatatc ctcaactgac agtcctgcat tacaaagttc cagaaagcgc cagacatagt
     4261 tacgctgaag ttgtcctcgc ttcagtccca accagacagt attagcatcg aaaagatgcc
     4321 gcgtatccag acggattaaa ttctcttcct cttgttcgcc gctggattgt tcggcaacta
     4381 atccgatccc aagcccaaga gcaacatagg ttttaatgac atcagaatcc tgcgcactta
     4441 atacaatatc tgccagcaaa cctttgcggg caaatgcgtc atcaatacgt gagcgccccg
     4501 taatcccccg tcggtaagtg attaacggcc acttcgctat tgattccagc gtcaatggtg
     4561 taatttgcgt taagggatga tcgagtggaa caagcaaact atggtgccaa cgaaaccacg
     4621 ggaaggcgac gagctgcggg tcattactca aacgctcgct ggcgatacca atatcagctt
     4681 cgccattttg caacaacgtc gcaatttcct gtggcgtccc ctggattagc tcgagccgaa
     4741 cctccgggaa aagttcgcga aaagctttaa tgacctctgg caagctataa cgtgcctgag
     4801 tatgcgtcgt tgcaatagtg agaacgccag acgtatcgtt ggtaaacagg tctgcaagcc
     4861 gacgaacatt actggcttca ttcagaatac gttctgcaat gaccagtaat gctttgcccg
     4921 gttcagtcat gcccagcagt cgcttacccc gtcgaacaaa tatttcgatg ccaagttcat
     4981 cctccagttc ccgaatatga cggctgacgc ctgactgtga ggtaaaaagc atattcgcaa
     5041 cctctgtcag gttgtaatcc tgacgtgcag cctcgcggat tatctttagt tgttggaaat
     5101 tcacggtaaa ctccgggcag ttcagatttc ccgttattgt taaagtctaa tgcccggcat
     5161 aacaaataat aaaaacccgc atcttattcc atcccgatat aacacttagc tcaccaattg
     5221 ccactgcctt ttttccatca ctggagaact aatcactgac attaacaact ctttcactgc
     5281 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
     5341 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
     5401 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
     5461 agactcaatt tcaccaataa cttttgccgt gagtcgccgt agggaaaaag cctcatcaac
     5521 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
     5581 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
     5641 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
     5701 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
     5761 gatctccgga aattcagcgc gaaccgcctg taataaggcc atggtgatgg atgacgcagc
     5821 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
     5881 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
     5941 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
     6001 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
     6061 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
     6121 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
     6181 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     6241 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     6301 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     6361 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     6421 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     6481 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     6541 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     6601 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     6661 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     6721 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     6781 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     6841 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     6901 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     6961 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     7021 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     7081 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     7141 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     7201 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     7261 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     7321 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     7381 gatcaggaac agttacagta aacgactgcc ccactaaacg gctaccctct ggaggtaatg
     7441 gataagttac cgccaggcta gtatggctgg caaaaagtag agcaaatgag caaaaaatat
     7501 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     7561 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     7621 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     7681 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     7741 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     7801 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     7861 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     7921 ccgaccaaat caaatccacc cgcttttgcc aggacatcaa cgccatcctg cggatttggt
     7981 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     8041 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     8101 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtaacaccg
     8161 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgcctgatgg
     8221 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     8281 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     8341 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     8401 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     8461 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcaaccccgg cattcctgcc
     8521 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtca
     8581 atatgccgtt gtgcacgcga catcgcggca ggatcgatgg cagggatcgt attcagtaaa
     8641 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     8701 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     8761 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     8821 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     8881 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgggcataa cgatgacgat
     8941 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     9001 tcggttcgcc acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa
     9061 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     9121 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     9181 ccccggtcat cagcgccagc acaagcacgc taaacagtgc cgccagtggt acgccacacc
     9241 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     9301 gaaaagtaat aataccgcga gaataatgct ggaaatccag tccctgggac cagcgacgcg
     9361 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     9421 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     9481 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     9541 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     9601 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     9661 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     9721 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccaacgctc
     9781 caccgtgcgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     9841 atcatcaagg atttgtgagg tggcgatata cagaacctgt gaagagtccc caataagcgc
     9901 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     9961 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
    10021 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
    10081 caatccatta cgaaagaaga tggctatgtg gaatagccag acttcctcgt gttaacgtct
    10141 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
    10201 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
    10261 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
    10321 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
    10381 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
    10441 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
    10501 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
    10561 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
    10621 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
    10681 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
    10741 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
    10801 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
    10861 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
    10921 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
    10981 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
    11041 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
    11101 ctggtttgcg aaaaacctaa attacgcgca atgctggcga tgctccagtc actatggcac
    11161 agtaactcac gagcgctggc catccgttgt tggtttaccc acgcattaaa accgattcca
    11221 tggtatttct tgaataactt actgaaataa taaggactta gataaacgtg cgaggcgaca
    11281 tcttccagac gcaaatcgtc tgataaatgc gcatcaatat agcgtaaagc ttttttcatt
    11341 ttattgtcgt ggggattagg cgcacgagtc tggcgcaccg aatctgaccg agatttgttc
    11401 tctttaataa ccacaaaatt gagttgtttt ttgaggcaat tttccacaat caacttcaac
    11461 aaatcagcag aggccataac tcttgagtaa tccatctcag gaactttgcg gaattcatta
    11521 agtaattcgg gatctgcctg ccagcaatca tcgacattca gaatatcgac caaatcgaca
    11581 tcatcattac gcagacgaac ttgcccgcac aatacaaaac cgactaaatg tccggcgatt
    11641 accagtggaa tagagaaatc ggttaatccg gcatgacagc gataaataca aggctgatcg
    11701 gatttagagg cttccaggcc cccacaacga tcgctcatgc gacatcgagt gcttagctga
    11761 gggtgttgcc gcatcatctg acaaaatgga gtgaagttaa acagctcgga aatttcatcg
    11821 ccgtgaatat tgaccacgac aaccgcaagg ctggtcgcct gcgcaaaatc ctgcgcgatt
    11881 ttattaatga gttctgaatt aagtgtcgcc gcag
//
LOCUS       DAZFRN010000145        11825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFRN010000145 DAZFRN010000000
VERSION     DAZFRN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.147"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            237..1625
                     /gene="narU"
                     /locus_tag="ACHKUB_003949"
     CDS             237..1625
                     /gene="narU"
                     /locus_tag="ACHKUB_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO6656907.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1707..5447
                     /gene="narZ"
                     /locus_tag="ACHKUB_003950"
     CDS             1707..5447
                     /gene="narZ"
                     /locus_tag="ACHKUB_003950"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO6656908.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5444..6988
                     /gene="narH"
                     /locus_tag="ACHKUB_003951"
     CDS             5444..6988
                     /gene="narH"
                     /locus_tag="ACHKUB_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6656909.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6988..7683
                     /gene="narW"
                     /locus_tag="ACHKUB_003952"
     CDS             6988..7683
                     /gene="narW"
                     /locus_tag="ACHKUB_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6656910.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7680..8360
                     /gene="narI"
                     /locus_tag="ACHKUB_003953"
     CDS             7680..8360
                     /gene="narI"
                     /locus_tag="ACHKUB_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6656911.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8440..9333
                     /gene="yddE"
                     /locus_tag="ACHKUB_003954"
     CDS             8440..9333
                     /gene="yddE"
                     /locus_tag="ACHKUB_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO6656912.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGVWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9429..10274)
                     /gene="nhoA"
                     /locus_tag="ACHKUB_003955"
     CDS             complement(9429..10274)
                     /gene="nhoA"
                     /locus_tag="ACHKUB_003955"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO6656913.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10447..11016
                     /gene="yddH"
                     /locus_tag="ACHKUB_003956"
     CDS             10447..11016
                     /gene="yddH"
                     /locus_tag="ACHKUB_003956"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO6656914.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11020..11247)
                     /gene="pptA"
                     /locus_tag="ACHKUB_003957"
     CDS             complement(11020..11247)
                     /gene="pptA"
                     /locus_tag="ACHKUB_003957"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO6656915.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11298..11825)
                     /locus_tag="ACHKUB_003958"
                     /pseudo
     CDS             complement(11298..11825)
                     /locus_tag="ACHKUB_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2795 a         3071 c         3077 g         2882 t
ORIGIN      
        1 ttgaaacatg acatacgttc aaagattaac ccccgtttga aggttagctt gttagttaca
       61 tttagtaaca catatctggc tacttaatcc ccattctgcc cccgcggtta cgctttctta
      121 tctattcttt caatcagcac ttcacccaat tcatccttaa ttctaataac tggctattgc
      181 gccttacctc ctgttttgtc ttttttcatt accaatgtgt gcatgtgagg aacaatatgg
      241 cactgcaaaa tgagaaaaat agtcgttatc ttttacgcga ctggaaacca gaaaatccgg
      301 ccttctggga aaataaagga aaacatattg ctcgaagaaa cctctggata tcagtcagtt
      361 gtctacttct tgccttctgt gtctggatgc tatttagcgc agttaccgtt aatctcaata
      421 aaatcggttt taattttact accgatcaac tctttttatt aaccgcatta ccctccgttt
      481 ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc tatattcggt ggacgccgat
      541 ggacggtttt tagtactgca atcctgatta ttccttgcgt ctggctcgga attgccgtgc
      601 aaaatccgaa tactcctttt gggatattta tcgttatcgc tttgctatgc ggttttgcag
      661 gtgcaaactt tgcttcgagc atgggcaata tcagtttctt ctttccaaaa gccaaacaag
      721 ggagcgctct tgggattaat ggcggattag gaaacttagg tgtaagtgta atgcagctgg
      781 ttgcaccgct ggtcattttt gtacctgtat ttgcctttct cggcgtcaat ggcgtaccgc
      841 aggccgacgg ttcggtgatg tcgctggcga atgccgcatg gatttgggta ccgctactgg
      901 cgattgccac gatcgccgca tggtcaggga tgaatgatat cgccagttca cgcgcctcaa
      961 ttgccgacca gctccctgtc ttacaacgcc tgcatctctg gctgctgagc ctgctttacc
     1021 ttgccacctt cggttcgttt atcggttttt ctgcgggttt tgccatgctg gcaaaaaccc
     1081 agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg cccatttatc ggtgccatcg
     1141 cgcggtcggt tggtggtgct atttccgata agttcggcgg cgtgcgggtg acgttgatca
     1201 actttatttt tatggcgatt ttcagtgccc tgctgttcct taccttaccg ggcacaggct
     1261 ccggtaattt catcgccttt tacgccgtat ttatggggct gtttctgact gcgggtctgg
     1321 gaagtggttc tactttccag atgatcgccg tcatctttcg ccagataacc atttatcggg
     1381 taaagatgaa aggcggtagt gatgagcaag ctcagaaaga agccgtcacc gaaacggcgg
     1441 cggctctggg ctttatctca gccattggcg cagtgggcgg cttttttatt ccgcaggcgt
     1501 ttggcatgtc gctcaatatg accggctctc cggtcggcgc gatgaaagtg tttttaatct
     1561 tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg tcggcggaag ttcagccaaa
     1621 aataagagat cgatgcctga tgtacaaccg tgtcgcatcc gacaaggcat cagatcccaa
     1681 ttcattcctg gagcaggagt catgtcatga gtaaactttt ggatcgcttt cgctacttca
     1741 aacaaaaggg cgaaaccttt gccgatggtc acggacaggt gatgcatagc aaccgcgact
     1801 gggaggacag ctatcgccag cgttggcagt tcgacaaaat cgtgcgttcc acccacggtg
     1861 ttaactgtac aggctcctgt agctggaaaa tctacgttaa aaatggtctg gtgacctggg
     1921 aaatccaaca gaccgactac ccgcgcactc gccctgacct gcccaatcat gaacctcgcg
     1981 gctgcccgcg tggcgcaagt tactcctggt atctttacag cgctaaccgc ctgaaatacc
     2041 cgctcattcg taaacgactg attgaactgt ggcgcgaagc cctcaagcaa cacagcgatc
     2101 cggtactggc gtgggcatcg attatgaacg atccgcaaaa gtgcctgagc tacaaacaag
     2161 tgcgtgggcg cggcgggttt atccgctcca actggcagga actaaaccag ctgattgccg
     2221 ccgctaacgt ctggaccatc aaaacctacg gcccggatcg cgttgccggt ttctcgccga
     2281 tcccggcgat gtcgatggtt tcttacgccg ccggaacgcg ttatctgtca ctgcttggcg
     2341 gcacctgttt aagcttctac gactggtatt gcgacctgcc gcccgcctcg ccgatgacct
     2401 ggggcgagca aaccgacgta ccggaatctg ccgactggta taactccagc tacatcatcg
     2461 cctgggggtc taacgtaccg cagacacgta cgccggacgc ccacttcttt accgaagtac
     2521 gctacaaagg cactaaaacc atcgccatta cccctgacta ctctgaagtg gccaaattgt
     2581 gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc cctggcgatg gcaatgggcc
     2641 atgtgatttt aaaagagttt catctcgata atcccagcga ctactttatc aactactgcc
     2701 gccgctacag cgacatgccg atgctggtaa tgctggagcc tcgcgacgat ggtagctacg
     2761 ttcccgggcg catgatccgc gcatctgacc tggtggatgg actgggcgaa agcaacaatc
     2821 cgcagtggaa aaccgtagca gttaataccg caggtgaatt ggtagtgccg aacggttcga
     2881 ttggtttccg ctggggagaa aaaggcaaat ggaatctgga atccattgcc gccggtacgg
     2941 aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc tgttgcaggc gtggccttcc
     3001 cctactttgg cggcattgaa aatccgcatt ttcgcagcgt aaaacacaat ccggtgctgg
     3061 tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg caacacctgt ccggtggtca
     3121 gcgtttatga tttggtactg gcgaattacg gcctcgatcg cgggctggaa gatgaaaaca
     3181 gtgcgaaaga ttacgctgaa atcaaaccgt acaccccagc ctggggtgag caaattaccg
     3241 gcgtgccgcg ccagtatatt gaaaccatcg cccgtgaatt tgccgatacc gcccataaaa
     3301 cgcatgggcg ctcgatgatt atcctcggcg caggtgttaa ccactggtat cacatggaca
     3361 tgaactaccg cgggatgatc aatatgctga tcttctgcgg ttgtgtcggg caaagcggtg
     3421 gcggctgggc acactatgtc ggccaggaaa aactgcgccc acaaaccggc tggttgccac
     3481 tggcctttgc gctcgactgg aaccgaccac cgcgccaaat gaacagcacc tcgtttttct
     3541 acaatcattc cagccaatgg cgctatgaaa aagtctctgc tcaggagtta ctttcaccgc
     3601 tcgccgatgc cagtaagtac agcggtcatc tgattgattt caacgttcgc gccgaacgta
     3661 tgggctggct accttctgcg ccgcagctgg ggcgtaaccc gctcgggatt aaagctgaag
     3721 ccgacaaggc cggattatcc cccacagaat ttaccgccca ggcgctgaaa tcgggcgatt
     3781 tacgtatggc ctgcgaacaa ccagatagca gcagcaatca tccgcgtaat ttgtttgtct
     3841 ggcgctctaa cctgcttggc tcctccggca aaggccacga gtatatgcag aagtatctgc
     3901 tggggaccga aagcgggatt cagggcgagg aactcggtgc cagcgacggg atcaaaccgg
     3961 aagaagtcga gtggcaaact gcagcgattg aaggcaagct cgacctgctg gtgacgctcg
     4021 acttccgcat gtccagtacc tgcctgttct ccgatatcgt tctgcccacc gccacctggt
     4081 acgaaaaaga cgatatgaac acctcggata tgcatccgtt tattcatccg ctttctgcgg
     4141 cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat ctacaaaggt atcgccaaag
     4201 cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac cgacgtggta ttacaacccc
     4261 tactgcatga ctctccggca gagctctcac agccgtgtga agtgctcgac tggcgcaaag
     4321 gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat tgtggcggtg gagcgcgact
     4381 accctgctac gtatgaacgc tttacctcgc tcgggccatt gatggacaaa cttggcaatg
     4441 gcggtaaagg gatttcgtgg aatacccagg atgaaatcga tttcctcggc aaactcaatt
     4501 acaccaagcg tgatggcccg gcacaggggc gtccgctgat tgacaccgcc attgacgctt
     4561 cagaagtgat tctggcactg gcaccggaaa ctaacggtca tgttgcagtc aaagcgtggc
     4621 aggcgctggg cgagatcacc gggcgcgaac atacccatct ggcgctgcac aaagaggacg
     4681 agaagattcg ctttcgcgat attcaggcgc agccgcgtaa aattatctcc agccccacct
     4741 ggtccggtct ggaaagcgat cacgtatcct ataacgcggg atacaccaac gttcatgagt
     4801 taattccgtg gcgcacgctg tcgggacgcc agcagctcta tcaggatcat ccgtggatgc
     4861 gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat cgacacccgt agcgtcagtg
     4921 agatgcgcca gataccgcca aacggcttcc cggaaaaagc acttaacttc ctgacgccgc
     4981 accagaaatg gggcattcac tcaacctaca gtgaaaacct gctaatgctg acgctctctc
     5041 gcggtggacc gattgtctgg atcagcgaaa cagatgcccg agaactgacc attgtcgata
     5101 acgactgggt ggaagtattc aacgccaacg gcgcgctgac tgcccgcgcg gtggtcagcc
     5161 aacgtgtacc gccgggcatg accatgatgt atcacgccca ggaacgcatt atgaatattc
     5221 ctggttcgga agtaactggc atgcgcggcg gcattcataa ctcggtcacc cgcgtttgcc
     5281 cgaaaccaac gcatatgatt ggcggttacg cgcagctggc ctggggcttt aactactacg
     5341 gcaccgtcgg atcgaaccgc gatgagttca tcatgatccg caagatgaag aacgttaact
     5401 ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa aaaatgaaaa tccgttcaca
     5461 agtcggcatg gtgcttaacc tcgacaaatg tatcggctgc catacctgtt cggtgacctg
     5521 taaaaacgtc tggaccgggc gcgaaggcat ggagtacgca tggtttaaca acgtcgaaac
     5581 caaaccgggc attggttatc cgaaaaactg ggaagatcag gaagagtggc aaggcggctg
     5641 ggtccgcgat gtgaatggca agatccgtcc gcgtctgggc agcaagatgg gcgtaataac
     5701 caaaatcttc gccaacccgg tggtgccgca gattgatgat tactacgaac ctttcacctt
     5761 cgactacgaa catttgcata gcgcaccgga aggcaaacat attcctactg ctcgcccgcg
     5821 ttcactgatt gacggcaaac ggatggacaa agtgatctgg ggcccaaact gggaagaact
     5881 gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac ttcgaggcca tgcaaaagga
     5941 gatgtacggg cagtttgaaa acaccttcat gatgtacctg ccgcgcctgt gcgaacactg
     6001 cctcaatccc agttgcgtgg cgacctgccc aagcggcgct atctacaaac gcgaagaaga
     6061 cggcattgtg ctgattgatc aggataaatg ccgtggctgg cgtttgtgca ttagcggttg
     6121 tccgtacaaa aaaatctact tcaactggaa aagcggcaag tcagaaaaat gtatcttctg
     6181 ttatccgcga attgagtcag gccaacccac cgtgtgctca gaaacctgcg tgggtcgcat
     6241 ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt gaggaagcgg cgagcaccga
     6301 gcgcgaagtt gacctctatg aacgtcagtg cgaagtattc ctcgatccac acgatccctc
     6361 agtgatcgag gaagccctga aacaaggtat tccacaaaac gtgattgaag ctgcccagcg
     6421 ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg ctaccgctgc accctgaata
     6481 tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg ccgattcagt cctacgcaga
     6541 tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc atcgaaagcc tgcgtattcc
     6601 ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc ggtccggtac tgcgggcgct
     6661 gaaacggatg atggcgatgc gccactatat gcgttcacaa accgtggaag gcgttactga
     6721 tactcgtgcc atcgacgaag taggcctgag cgtcgcccag gtcgaagaga tgtatcgtta
     6781 cctcgccatt gccaactatg aagatcgttt tgtcatcccg acgagccatc gggaaatggc
     6841 gggcgatgcc ttcgcagaac gcaacggctg cggttttacc tttggcgacg gttgccacgg
     6901 ttcggacagt aaattcaacc tgttcaacag tagccgtatc gatgccatca acatcaccga
     6961 agtgcgcgac aaagcggagg gcgaataatg cagatcctca aagtgatcgg cctgttgatg
     7021 gagtatccgg atgagctgtt gtgggaatgc aaggaggacg cgctggcgtt gatccgccgc
     7081 gacgcgccga tgcttacgga tttcacccac aacctgctta acgcgccgct gctggataaa
     7141 caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca cgtcgctgct gctgttcgaa
     7201 catgttcatg ccgagtcccg cgatcgcggc caggcaatgg tggacctgct ggcggagtat
     7261 gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg attatctacc gctgtatctg
     7321 gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag gattgctcaa tgttgcgccg
     7381 atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg caccgtggta cgcgttgttt
     7441 gatgctctgc tgcaactggc gggaagtata ctttcaagtg acagtgtcac aaaacaagta
     7501 aacagcgaag agcgcgatga cacccgccag gcgcttgatg cagtgtggga agaggaacag
     7561 gtgaagttta ttgaagataa cgccacggcg tgtgacagct cgccgcttaa tcaatatcag
     7621 cgacgcttta gccaggatgt cgcgccgcag tatgtcgaca tcagtgcggg aggtgggaaa
     7681 tgattcagta tctgaacgtc tttttttacg atatctaccc gtacatttgt gcgacggtgt
     7741 ttttcctcgg cagctggctg cgctacgact acgggcagta cacctggcgc gcctcctcaa
     7801 gtcaaatgct cgataaacgc gggatggtga tatggtcgaa tctgttccat atcggcattt
     7861 tggggatttt cttcgggcac ttgttcggca tgttaacgcc gcactggatg tacgcgtggt
     7921 ttttgccagt ggcagcgaaa caactcatgg cgatggtgct cggcggtatt tgcggtgtgt
     7981 tgacgttgat tggcggcgca ggactgctgt ggcgcaggct gacaaatcag cgcgtgcgtg
     8041 caacttccac tacgccggat attatcatca tgagcatttt gctgatccag tgtctgctgg
     8101 ggctaagtac cataccgttt tcagcacagt atcctgatgg tagcgaaatg atgaagctgg
     8161 tgggttgggc gcaaagcatt gtcactttcc gtggtggctc gtcagaaatg cttaacggcg
     8221 tagcgttcgt tttccgcctg catctggtat tgggaatgac catcttcctg ctcttcccgt
     8281 tcaccagatt ggtgcatgtg tggagcgcgc cgtttgagta ctttactcgt cggtatcaga
     8341 ttgtgcgttc gcggcgataa ttttcaccct ggccggatag cccttcgctc tccggccact
     8401 tgcaggctat gatagttctc cagccacagg agaacgttaa tgaaaccgca ggtgtaccac
     8461 gtcgatgctt ttacctcaca accgtttcgc ggcaattctg ccggtgttgt tttccccgcc
     8521 gataatctta gcgaagcgca aatgcagctt atcgcccgcg agttaggcca ttcggaaact
     8581 gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc gctactttac gccaacggtt
     8641 gaagtgccga tttgcggtca cgcgacagta gctgcgcact atgtacgtgc gaaggtgtta
     8701 ggtttaggaa attgcacgat ctggcaaaca tcgctggcag gaaaacatcg cgtgactatc
     8761 gaaaagcaca acgatgatta tcgtatttcg ctggaacaag gtacgccggg ctttgagcca
     8821 ccgctggaag gtgaaacacg tgaggcgatt atcaacgcgc ttcatctcac tgaagacgat
     8881 attttgccgg gcttgccaat tcaggtcgca acaacaggac actcaaaagt gatgatcccg
     8941 ttgaaaccgg aagtggatat cgacgccctt tcgcctgatc ttgctgcgct gagcgctatc
     9001 agcaaacaga ttggctgcaa tggtttcttc ccgttccaga tccgcccagg caaaaacgaa
     9061 accgatggtc gtatgttctc gcctgcgatt ggtattgtgg aagatccggt gactggcaat
     9121 gccaatggcc cgatgggtgt atggttggta catcacaacg tattgcccca cgatggcaac
     9181 gtgttatgtg ttaaaggcca tcaggggcgc gcattggggc gtgacggcat gattgaagtg
     9241 acggtgacaa ttcgtgataa ccaaccggaa aaagtcacca tttcaggcac agcggtgatt
     9301 ttgtttcatg ccgaatgggc aattgaactc tgaaaccgtt gcctgatgcg cttcgcctgt
     9361 caggtctaca accgagctaa tgtatgccgg ataaggcgtt tacgccgcat ccggcaacct
     9421 gacataaatt attttcccgc ctccggatgc gtatcaaacg ccgccatcac cagcgctaac
     9481 tcatccacag taaaaccatg tttcgcatca tccacgccca gaccaaactg ttcctgcatc
     9541 acggcatata acgacaccac atccggtaga tttcgctgct ccaccgcgtg cccattttca
     9601 taatgggtaa aatgaaaatt ggtcagcgtt agcttgccac cgtccggcaa atggcggcac
     9661 atcagcaaat gatggcgaaa atgcgactgc ggccagtgcg ccgaccagaa attgcccatc
     9721 acataatcgc tttgttgctg ctcgcagaga tcaaaacggt acatcgactg ccagtgctga
     9781 tgatgattaa actgcaacac ccaatcatca ccctcctgca acaaccgata atctccgtgt
     9841 ggcgtggtct gcacgagatc ggaaactaaa cgaatcggcg cggttagcgt ctgcccaccg
     9901 aaaccgacat cagcaatcca tttttcctct tccagttcca ccaacagcaa acgatgggtg
     9961 cgcggcggta atgctggcgg atttgataac actacgcgcc ctaacaagct gcgaacgtta
    10021 aactccagct cgcgtaacac ccgctcaaac acgccattct gctcaaaaca gtaaccgcca
    10081 cgacgggcta tcaccagttt ctcttccagc gattgatcat caagctgtat ttccctcggc
    10141 agcaaaacgt cgaggttttc aaacggaata gtgcaattgt gtttcaggtg caatgcacga
    10201 agcgtatcaa tattgaccgc agcagctccc gaccagttaa tacgggcaaa atagtgattc
    10261 agaatgggcg tcatcaggcg tttcccttag tggttttctc aacttataac ctggaataga
    10321 cgccgcaccg gctattttgt gcgatcgcgg acatgcatca cgtttgcaaa tcgccaatcc
    10381 cgttaatttc gttacttatt gcgttacgga ctacgcttaa aaaaaccttc ttcgcgaggc
    10441 gataccatga gccgattcat ccccatagaa ttacaccatg ccagccgtct gttaaatcat
    10501 ggcccaactg tcatgatcac cagtttcgat gagcaatccc agcggcgtaa cattatggca
    10561 gcagcctggt caatgccggt ggagtttgaa ccgccacgcg tggcgattgt ggtagataaa
    10621 tcgacatgga ccagggagtt gattgaacgt aacggtaaat ttggcatcgt tatcccgggc
    10681 gttgcagcaa ctaactggac gtgggcagtg ggaagcgtgt cgggtcgtga agaagataaa
    10741 tttaattgct atggcattcc ggtggtgaaa ggtccggtgc ttggtttgcc acttgttgaa
    10801 gagaaatgcc tggcgtggat ggagtgccgc ctgctacctg cgacttctgc gcaacaaaaa
    10861 tacgacacgc tgtttggcga ggtggtatca gcagcggcag acgcacgggt atttgtcgaa
    10921 ggccgctggc agtttgatga tgataagctc aatacgttgc atcatttagg tgctggcacg
    10981 tttgttacca gcggcaagcg agttacggcg ggttaagcat tacatgctgt aaccgggttt
    11041 ctttatcaaa gcctccattt ggggcgcgat ttcggcatcc cagatagctt gccaagattc
    11101 tggttgaatc tgctgtagag caatgcttat cgagctatct ttactgttca gatggcgaat
    11161 aataacgtcg gtaatatctg cagcaagtgc tgctttttgt tgttcgtcca gttcacgcgg
    11221 aaaacattta atgtcgatgt gcggcatgat tgtgctcctt ttgaaagatg tcagtaaaat
    11281 taacacacat tgtgaatttt cagtcctttc cattcaaacg tgaaatcaat aagttcatca
    11341 gggacatcgc aaacaatatg aatacggatt tcttctctgc cgtgactctt ttcacaaatt
    11401 gcgtaactgt catgctctgg attatttaat tctttcagcg gaaatttttc ctcaaaggct
    11461 ttatttagcc acccctggtt tcctttcacc gcgaataaat aatcacctcc ctgtttttgt
    11521 atcttctctg ctatatcttt ctggcaaccc atcgcatcag ttgtgatgat ttttccttta
    11581 atatccagca tgttaagaag ttctgggata gctgtaatct cattagattt atcatccgtc
    11641 ttgatctgtc cgatgaccag actgtgcatt gttgagaacg cactaatgac atgaatcgct
    11701 cccctgcggc gactcttatc ataagaatgc cggagcgttt ttccatcaat tgcaatgacg
    11761 tctttatcat ctgaagaatg acaatcacgc atccagttaa taaagcactc atgaaatttt
    11821 gtagg
//
LOCUS       DAZFRN010000146        11813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFRN010000146 DAZFRN010000000
VERSION     DAZFRN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.148"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            223..873
                     /gene="csgD"
                     /locus_tag="ACHKUB_003959"
     CDS             223..873
                     /gene="csgD"
                     /locus_tag="ACHKUB_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO6656916.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            878..1267
                     /gene="csgE"
                     /locus_tag="ACHKUB_003960"
     CDS             878..1267
                     /gene="csgE"
                     /locus_tag="ACHKUB_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO6656917.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1292..1708
                     /gene="csgF"
                     /locus_tag="ACHKUB_003961"
     CDS             1292..1708
                     /gene="csgF"
                     /locus_tag="ACHKUB_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO6656918.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1735..2568
                     /gene="csgG"
                     /locus_tag="ACHKUB_003962"
     CDS             1735..2568
                     /gene="csgG"
                     /locus_tag="ACHKUB_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO6656919.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2632..3123)
                     /gene="ycdZ"
                     /locus_tag="ACHKUB_003963"
     CDS             complement(2632..3123)
                     /gene="ycdZ"
                     /locus_tag="ACHKUB_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6656920.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3225..3779)
                     /gene="ycdY"
                     /locus_tag="ACHKUB_003964"
     CDS             complement(3225..3779)
                     /gene="ycdY"
                     /locus_tag="ACHKUB_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO6656921.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3803..4540)
                     /gene="ycdX"
                     /locus_tag="ACHKUB_003965"
     CDS             complement(3803..4540)
                     /gene="ycdX"
                     /locus_tag="ACHKUB_003965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO6656922.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4595..5533)
                     /gene="ghrA"
                     /locus_tag="ACHKUB_003966"
     CDS             complement(4595..5533)
                     /gene="ghrA"
                     /locus_tag="ACHKUB_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO6656923.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5767..5854
                     /locus_tag="ACHKUB_003967"
     tRNA            5767..5854
                     /locus_tag="ACHKUB_003967"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5801..5803,aa:Ser,seq:gga)
     gene            complement(6004..6846)
                     /locus_tag="ACHKUB_003968"
     CDS             complement(6004..6846)
                     /locus_tag="ACHKUB_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6656924.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQCS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEENILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDQWGLHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSLQGKECYEDEMFIIKYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6931..7128)
                     /locus_tag="ACHKUB_003969"
     CDS             complement(6931..7128)
                     /locus_tag="ACHKUB_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6656925.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(7140..7631)
                     /locus_tag="ACHKUB_003970"
     CDS             complement(7140..7631)
                     /locus_tag="ACHKUB_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6656926.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINVVCDNGY
                     SLRVVDKSNQQSTSTPPPFTALAGIRCSTAHITAKDNAWLYSLSHQTSDTGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPT
                     FDW"
     gene            complement(7628..8002)
                     /locus_tag="ACHKUB_003971"
     CDS             complement(7628..8002)
                     /locus_tag="ACHKUB_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6656927.1"
                     /translation="MKTLPDTHVREASRCPSPVIIWQILLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYLEAK"
     gene            complement(8092..8445)
                     /locus_tag="ACHKUB_003972"
     CDS             complement(8092..8445)
                     /locus_tag="ACHKUB_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6656928.1"
                     /translation="MSDTLPDGNHDRPWWGLPCTVTPCFGTRLVQEGNRLHYLADRAG
                     IRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDADTLGSC
                     GYVYLAVYPTPETKK"
     gene            complement(8521..8742)
                     /locus_tag="ACHKUB_003973"
     CDS             complement(8521..8742)
                     /locus_tag="ACHKUB_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6656929.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8811..9287)
                     /gene="yeeS"
                     /locus_tag="ACHKUB_003974"
     CDS             complement(8811..9287)
                     /gene="yeeS"
                     /locus_tag="ACHKUB_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6656930.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(9303..9788)
                     /locus_tag="ACHKUB_003975"
     CDS             complement(9303..9788)
                     /locus_tag="ACHKUB_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6656931.1"
                     /translation="MTTFSHNSTTPSVSVTTASGNNQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNV
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9880..10698)
                     /locus_tag="ACHKUB_003976"
     CDS             complement(9880..10698)
                     /locus_tag="ACHKUB_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6656932.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTTDILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11025..>11813)
                     /locus_tag="ACHKUB_003977"
     CDS             complement(11025..>11813)
                     /locus_tag="ACHKUB_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6656933.1"
                     /translation="LEGDLMRTEVAGMSLTTGVYGAAGHSSVDVKDDDSSRAGTVRDD
                     AGSLGGYLNLIHNASGLWADIVAQGTRHSMKASSDNHDFRARGRGWLGSLETGLPFSI
                     TDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTS
                     SRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSL
                     DLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT         2938 a         2986 c         2974 g         2915 t
ORIGIN      
        1 attttaatat aacgagttac atttagttac atgtttaaca cttgatttaa gatttgtaat
       61 ggctagattg aaatcagatg taatccatta gttttatatt ttacccattt agggctgatt
      121 tattactaca cacagcagtg caacatctgt cagtacttct ggtgcttcta ttttagaggc
      181 agctgtcagg tgtgcgatca ataaaaaaag cggggtttca tcatgtttaa tgaagtccat
      241 agtattcatg gtcatacatt attgttgatc actaaaccgt ctttgcaagc gacagctctc
      301 ttgcagcacc ttaaacaatc gctggcaatt acaggaaaat tacataatat tcaacgttct
      361 ctggacgata tatcttcagg ctctattatt cttctggata tgatggaagc agataaaaag
      421 cttatccatt attggcagga tactttgagc aggaaaaaca acaatatcaa aatattgttg
      481 ctaaatacgc ctgaagatta cccgtaccgc gacattgaaa actggcctca tatcaacggc
      541 gttttttatg ccatggagga tcaagaacgt gttgtcaatg ggttgcaagg cgtcctgcgc
      601 ggcgaatgct actttacgca aaagcttgcc agttacctga ttacacattc tggtaactat
      661 cgttataaca gcacggaatc agccctcctt actcatcggg aaaaagagat cctgaataag
      721 ctgcgtatcg gcgcgtctaa taacgagatc gctcgttcgt tgttcatcag cgaaaatacg
      781 gttaaaacgc atctttataa tcttttcaag aagatagccg taaaaaaccg gacacaagcg
      841 gtttcctggg caaacgataa cctcaggcga taaagccatg aaacgttatt tacgctggat
      901 tgtggcggca gaatttctgt tcgccgcagg gaatcttcac gccgttgagg tagaagtccc
      961 gggattgtta actgaccata ctgtttcatc tattggccat gatttttacc gagcctttag
     1021 tgataaatgg gaaagtgact atacgggtaa cttaacgatt aatgaaaggc ccagtgcacg
     1081 atggggaagc tggatcacta taacggtcaa tcaggacgtt attttccaga cttttttatt
     1141 tccgttgaaa agagacttcg agaaaactgt cgtctttgca ctgattcaaa ctgaagaagc
     1201 actaaatcgt cgccagataa atcaggcgtt attaagtacg ggcgatttgg cgcatgatga
     1261 attctaaata aaaaattgtt cggaggctgc aatgcgtgtc aaacatgcag tagttctact
     1321 catgcttatt tcgccattaa gttgggctgg aaccatgact ttccagttcc gtaatccaaa
     1381 ctttggtggt aacccaaata atggcgcttt tttattaaat agcgctcagg cccaaaactc
     1441 ttataaagat ccgagctata acgatgactt tggtattgaa acaccctcag cgttagataa
     1501 ctttactcag gccatccagt cacaaatttt aggtgggcta ctgtcgaata ttaataccgg
     1561 taaaccgggc cgcatggtga ccaacgatta tattgttgat attgctaacc gcgatggtca
     1621 attgcagttg aacgtgacag atcgtaaaac cggacaaacc tcgaccatcc aggtttcggg
     1681 tttacaaaat aactcaaccg atttttaagc cccagcttca taaggaaaat aatcatgcag
     1741 cgcttatttc ttttggttgc cgtcatgtta ctgagcggat gcttaaccgc cccgcctaaa
     1801 gaagccgcca gaccgacatt aatgcctcga gctcagagct acaaagattt gacccatctg
     1861 ccagcgccga cgggtaagat ctttgtttcg gtatacaaca ttcaggacga aaccgggcaa
     1921 tttaaaccct acccggcaag taacttctcc actgctgttc cgcaaagcgc cacggcaatg
     1981 ctggtcacgg cattgaaaga ttctcgctgg tttataccgc tggagcgcca gggcttacaa
     2041 aacctgctta acgagcgcaa gattattcgt gcggcacaag aaaacggcac ggttgccatt
     2101 aataaccgaa tcccgctgca atctttaacg gcggcaaata ttatggttga aggttcgatt
     2161 atcggttatg aaagcaacgt taaatcgggc ggtgttgggg caagatattt tgggatcggt
     2221 gcagacacgc aataccagct cgatcagatt gccgtgaacc tgcgcgtcgt caatgtgagt
     2281 accggtgaga tcctctcctc ggtgaacacc agtaagacga tactttccta tgaagttcag
     2341 gccggggttt tccgctttat tgactaccag cgcttgctgg aaggggaagt gggttacacc
     2401 tcaaacgaac ccgttatgct gtgtctgatg tcggctatcg aaacaggggt cattttcctg
     2461 attaatgatg gtatcgaccg tggtctgtgg gatttgcaga acaaagcaga acggcagaac
     2521 gacattctgg tgaaataccg ccatatgtcg gttccaccgg aatcctgaca gataaaaaag
     2581 ccggaattat catccggcat tttactgttt aagcgaaagc gtgagtgccc ctcacgcttt
     2641 atttttgatt tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca gccacaggcc
     2701 gctgtttttc attgcgtaac caaataccag cccaagcgcc agcgaaggta acaccagttt
     2761 ccagtcaccc tgcccggcaa acgtcgcgca tgcgcctata aacgtccccg gaacaaatga
     2821 aagcagcagc tgtttggcct gaatacacat cagaaacgcg acaataccgg ttatgacata
     2881 accgagaatt tccagatgtg gtgccagtgc actaccgtaa ataatgacca tcgcccacac
     2941 cacgccacta agcagggttg cagcggagat cgccagccct ttcagcccac cttgcgggca
     3001 ggcaaagtag gccgtacagc ccaggaagcc cgcccagctt agtaagccca gggaaacagc
     3061 cacccatccc cagataccgg agagaatgcc tgttgtgatt gcaatagaga gaagtatgtt
     3121 catgccgcgc atgatagcag aaaacgcggc catgaatgag atcgagcaca cattttatgc
     3181 aacaacaaat tatctgttgc agttatgagt gacgatgatc acatttactc ttcagaatct
     3241 tcttccagct cgtcccacat tgcactaatg gcatcacggg ttagcggtgc catggtgcgc
     3301 cagaaaggcg tggttgcatg ggcctccact ttgccgagga acgcaccaca ccagggtaac
     3361 agatactcac tgaacagtgt ttccagtgct tcgctctcat cttccgttga ctgatcttcc
     3421 agccaggaag ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc cgccagtggc
     3481 atccctcgtt cggaaagaaa agcgcgcact tccgcttccg ttgcgtcctc aacccatgcg
     3541 ctacgatatg gcggcacggc acattcatcg ccgataaaca acgcattgta atcggcagag
     3601 acttgcgcca tatcacagct tttctgtaaa cgcgtcagta actcatcctg ctccagtggc
     3661 cagttcgcag ccagtttccc ctcacgaatc agggtaaaca gcggcaccag taaaggatct
     3721 tgcggttggc ggtaatagag cgaacccagc acacgacaga ggatagaaaa ctcgttcatt
     3781 aatatatttc cattactagt aattaaaggt ctgcaaattc cgcaatcggt gccataccgc
     3841 gagattcaag gaagttcagt aagcggcgcg gcgaaacatt caaaatgcgc tctggcggaa
     3901 aatctaccgc gtcgaggatt ttaagacact cttcaaattc ccccatggta aacgcggtgt
     3961 gagaatccga gcctaatgcc acccaacctc cagcatcacg taccgctgcg gctaccgcac
     4021 gacagttgtc ttcactgccc ttacgtgagt gtaaaaatga agaattattg atttccagcg
     4081 ccacctgatg tttcgcggct gcttcagcaa cggctttcac atctatttca tatttgggat
     4141 ttccgggatg gcttattata tgcacattgc cgctggcgat agtggcgatc atcgcttgtg
     4201 tattggtcgc tttgtcatgt ggtgcaaaaa ccggctcatg aaaaccggca ataatcagat
     4261 ccagcgagtc aaacatttta ccgctgcaat caatttcacc atcaacattt ttaatgttag
     4321 cttcgatgcc gcgcaggatc cctaccccat caaccactcg cggccagata cgcatgttaa
     4381 tgaagtgcca gtgatgcgga gcatcttcca tatccgggcc atgatcggtg atcgcaaaaa
     4441 gtttaatgcc cttttgtttg gcctgggcaa tgtaatcact caatgtgcta tatgcatgtg
     4501 tgctggcaac ggtatgcata tgaaggtcga cgggatacat aactctctcc tgtattcaat
     4561 tcccgccaag gatagcagga atcctgatgc tttattagta gccgcgtgcg cggtcgactt
     4621 gcccgcagac cctctcccct ttttcgagct gggcaatggt gcgagaaatg tactccacag
     4681 cttcagcggg acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt ggatgttgcc
     4741 agagcggact ttcaggcggt aagggttcac gattaaaaac atccaacatt gcgcctttaa
     4801 ctttgccgct atccagcgcc gcgagcaggt catcttccac aacatgaaca ccacgcgcca
     4861 ggttgaggag atacgcgcca tccggtaatt tttcgagtaa ttgttgatta ataatgccga
     4921 cggtttcagg ggtattcggt aacaaattaa tcaatacccg acattggctc agaaatgcag
     4981 acagttcttc ccgtccggca aagctttgca cgccaggcca cgatttacgg gttcgactcc
     5041 agcaacgcag cggaaagcgc caggtttgca gactctgagc aactttactg cccagtacgc
     5101 ctgcgcccaa aatgccgatg gtaaaatctt cccgatgata ttcaggcagc ggttgccaat
     5161 gcgaactatt ttgctggatg cgataatcgt caaaacgtcg aaaccaatgc agcacctgac
     5221 tgacagcata ttcctgcatt tgctcgccca taccggtatc ttccaggcga aaaagtggaa
     5281 cagaagggtt cagcatttca gggtgtgcct gtagcttgct caaaatagaa tcaacaccgg
     5341 ccccgagtgc gaacaccgct ttaagatcgc gccctgccag catttcaaca ggaggatgcc
     5401 agactaaagc ataatcagca gagtcattat ctccgctttt ccacgctctg actcttgcct
     5461 gaggaatagc tttgcgcagt gcctcaatcc accattgggt atcgaacgtt gggtgataaa
     5521 agatgatatc catactgact cccgaaaagc gttgtgcgaa atttattcgc acttatcgtt
     5581 atgatctaca aaggccacca gcataacaaa tccgtggtcg gtggcaaaaa aagcagattt
     5641 cgcttattaa aaccacacat tgattgaagt ttgaataaac gcgcgatttt ttcaaaaagt
     5701 ttgttgacct caggtcatga tttccctaaa ttagcgcccg ttccagcaag acaggaacga
     5761 caatttggtg aggtgtccga gtggctgaag gagcacgcct ggaaagtgtg tatacggcaa
     5821 cgtatcgggg gttcgaatcc ccccctcacc gccagattac aagcgcatgt tattgtttaa
     5881 catgcgcttt tttatttatc cttccagaca gtatgccacc gggtatgcca tctgaaaccg
     5941 gtttatatgc cccacgatca tatcttatcg gacaatttcc gatctttgag catactggca
     6001 aagctatact gccagcatgt caggatagtg ttttgcgata atatcattca gtctgtcaac
     6061 cagttctggc ttcctgaacg tgatgtgcgc agagcccttc ttaaagtatt taataatgaa
     6121 catctcatct tcatagcatt cttttccctg aagagaccgg atgtgatcgt ccagatgaat
     6181 agttatgttt tccctgttgt cggggatcgg tttgccgctg aacagatgca gcattctttc
     6241 caggtcggca agtctatctg cctgttgccc ggtgataaga tgcaatcccc actggtccca
     6301 cctcaccaga ttgttgacga taattttatt gccaaattta caggggcaat tggttttgta
     6361 attccagctc aaccctctga agaggttgat cactcctcgc tcaaacactt catccttatt
     6421 ctggtgtagc tgctcaaacg tactcagaat attctcttca ctgatttccg gaaaattatc
     6481 gtactccagt gaccggtacc atgtatcacg ggcctgtgca tccattaagg aaagcatccc
     6541 ggagcattgc atcaggtcac gccagatttc tcgatcgaga ttgcgggtga tggctttcat
     6601 tgccgtttca ggtttttcca tcagccagca accgcagcga aaatcctgtt tcattgccca
     6661 gtccagtgcg gttttaccgc caatactttc tgtcattgct gatatttcag ccagttgccg
     6721 gataagtgtg tcgatttgca gcagtgtact ctttcgtaca gccagaatgt ggcggatact
     6781 ggttgagcag ataacgtccg tatgcccggt gaccagttcc ggacgggaga ttgtgatgtc
     6841 tgacattatc ggtttccctt tgggtttata aacgaaaacg ccagccctgt tcaggctgac
     6901 gtcagtggaa tgaagtctgg tgagtgagat tcaccggttc tggtgcagaa gctgcagttg
     6961 gcgcagggta cggatatcct ccctggcctg ttcaatacag ggcaacagtg ctgccgaatc
     7021 cgttttatct tcatcgttgt caaagataat tcccgattcg cagtcgatat tgtcctgcag
     7081 ccatgcaatc agaatatcca gcgctgtttc cgtggttaat gatttcatgt tgtttctggt
     7141 taccagtcga aagtggggaa acccggcaga catccggcac tggcatccag atgaatgaga
     7201 ctgacgccat aacgtcggat gagtgtgaca accagacggc ggaacgtttt tgacagcccc
     7261 aggcgtttaa gccgcagaac cgggtatgac cacgcatcgg tacgtaacag atatccgcta
     7321 cctgtgaaat gaatccattc tgattcgccg gtgtcactgg tctgatgtga cagcgagtac
     7381 agccaggcgt tgtcctttgc cgtgatatgc gcggtactgc agcgtatgcc agcaagggcc
     7441 gtaaacggtg gtggtgtact ggttgactgt tggttggatt tatcgaccac gcggagtgaa
     7501 taaccgttat cgcagaccac gttaatcagt tcagcgagat taacaccatc aatgtcaacg
     7561 acaatgcgcc ccatgttcag tgcctgtacg ttaacgctgt cggcttccag tgtcagggcc
     7621 agtttcatca tttcgcctcc agatacttac ccagggtaat gttatttacc gttctgtaat
     7681 tgtcgcgggt catcagacct gttgcccggc gtgcccggag aatatcaatg ctgttgatta
     7741 actgcgacga agttcctgcg ctgaatcccg gctggtcggt acgtaccagt gcgtattttt
     7801 caacgagaaa attcactgca tcacacagtg aaatgcctgc ctcaatatgc tgctcaatca
     7861 cacgttcatc ggcaaacggt gtgtcattca gcgtaaggcc gtaatgctgg tccagcagtc
     7921 gggtgagcag aatctgccag atgatgacgg gagacgggca gcgcgatgcc tcccgtacgt
     7981 gtgtatcggg taatgttttc atactgaaga ttttcctgat atgcaaagat gaaaaatggg
     8041 aaagtggtgt ggtgaaagca ccagaccgga gcagacgatt attcctgtgg tttacttttt
     8101 cgtttcgggg gtcggataaa cagccagata aacgtaacca caggagccga gggtgtcggc
     8161 atcgcaggtc agccctttag catatagcgt gacggtatgc tgatggcggg gattcagttc
     8221 accgctggtg agcatgagtt ccagttgttt catcagcagc ggaaaggcct ggtccaggtg
     8281 gtatgcatct gcgtcgctga agcggcctct gataccggcg cggtcggcaa ggtaatgcaa
     8341 ccggttaccc tcctgcacca gacgggtgcc gaaacagggc gtcacggtgc agggcagccc
     8401 ccaccagggg cggtcgtgat tgccgtcggg aagtgtgtct gacacgataa aatcctcaca
     8461 gaaaatcggt taagaatggt ccggtggtgg cgataattct gctcatcaga atgccctgac
     8521 tcagttcagg gtgacgctca tcagccggac atacgggcca aaactgtcct tacggcgttc
     8581 agcaaacacg gccagcacac cgggaatatc ctgtacttca cgaccggtat acgccccggc
     8641 actgccgtgc cagcggtact taccggtgca gaacggaaaa agacgggagg ccggatgctg
     8701 ttggtgaata cgcatggctt caccacgggt gatgattttc atgatgtgat acctctgaag
     8761 acagaagata aaagtgaaaa caggtatgat gtggttgtga cggtgacggg ttaaagcaga
     8821 ccgtgttcgg caaaggagaa aacctggctg ccaccgacta tcagatggtc cggcacccgg
     8881 atatccacca ggcccagtgc ctgtaccaga cgttccgtaa taagccggtc tgccttactg
     8941 ggtgtgactt caccggacgg gtgattgtgt gccagtacca cggcagcggc attgtgatac
     9001 agggcgcgtt taatcacttc ccggggatgg acttccgtgc ggttgatggt accggtgaag
     9061 agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg gaactcttca
     9121 cgctccagtc ccgccatgtt cagaatcagc cattcccgtg ctgcacgggt ggaggtgaag
     9181 gccacgccgg gttcatgaag atggcggtcc agggttttca gggcccgttg aatgaggctg
     9241 cgctcgccgg gcgtcatctc tccgggcaga aaggaaagct gctgcattgt ccctgcctcc
     9301 attcagtcga tgatgcgcat aatggcgctg cattccggat gctgcagggc ataatcccgc
     9361 agccggtaat aatgaaccgt catggcataa ttctctgtac gacaggcgtg atggctgtac
     9421 gccatcagac aggcggcaat gccggcggct tccgggctca tttcagcgcg gttaccgttc
     9481 atgacgttaa acagcaccca tgtttcgtca tcatcgtcat ccggttcggg tgccataaat
     9541 gcgccaccgt tgttcagggt gtacagattc cagatgcccc cgcagtagtc ttcacacaga
     9601 cggtccatcc agccgaagac acggggctcc agggtcaccc actgtggaat gaggccaaag
     9661 tgctgcggcc agaagctgat gcgctgctca tcagggacgg gagtggcaac cagttgagac
     9721 tggttattcc ctgatgcagt agttacggaa acagaaggtg tggtggagtt atgcgaaaag
     9781 gttgtcatga gatgattcct tataaaaagt aaatgaatgg aagaatgcct ggggactggt
     9841 acagacgtga gtcagggctg cactttcagg gaaacagcat cagcgcatgc tctccagcag
     9901 cgtttctgcc atcacccaca acgcccggtt gagcttaatg tcggtatcga tgctgtgaat
     9961 ggcccgggta tggatacgtt ttcctctggc actgcgaccg gaaatcccgc ctttcagcat
    10021 attttcctgg atggtctgat aagcactcca caggtcctta ccgtaatcct cccggcgtcg
    10081 tggcgtcaga atgtcggtag tggtgacggg ctgatgttca tcaccataac ggtaagtcag
    10141 cgccgcctgt gccagtgcct gacgtgccgg tggcggcaga accagagact gcatggcatc
    10201 acgcttttcc tcaatccggt caaaaacgcc cactacctcg taagcccctt caatgactct
    10261 gtccaccaca tttccccggt ggggcacacg gacttccccc agagactgac cgcagacgca
    10321 gccgttctgg cagacgaatc tgaagtaacc cggcagcatc tggtagctgg aggtaccgtc
    10381 atgggagttg agcagaataa tttcaggcac atgctgaccg gttatctgtc cggcccgccg
    10441 cagacgcagc atgtgtttgg tatattcccg gcggcccggg tcacgtacgc gggtctggca
    10501 ggcgaagaat ggctgaaagc cttcccgctg caggctttcc agcacggtga tggtgggaat
    10561 ccaggtatag ttctcgcttc tggatgtgtg tttctcttcc ccgaatacgc tggggacaac
    10621 ctgcatcagt tcttcacgag ttaaggggcg gtcacggcgt atctggttcg cataaccaaa
    10681 acgactggct aatcgcatga tttattcctt atgaatgagt tagcggtata aatgaaaaag
    10741 ccgcgtctcc cggagaagac gcggcctgat gggggaaatg aatgacgttt attgtctgag
    10801 aagcccttca ctggcgagct gcgtattttt atggaaggag atcacccaat aaccccatcg
    10861 ggatcgggta ataaccggcc agactgtgat cgattcagat gccatttggc agcaaaagag
    10921 ccttcggcaa taaacgtgct gtacaacccg gcatatctgt tgctccctga aagtaaaaag
    10981 ccccggtcat gatgaccggg gcctgaaggg gagtgacctg attatcagaa agtcacattc
    11041 agcgtggcct gaccgttata gccttcagcg ctgctgccgc tgacgctgtg ggcataaccg
    11101 gcctgaacgc ccagggtgat attttcccgg acacgggctt ccagtccggc ctgcaggtcc
    11161 agtgaagtgc cattctgtga gggagagaac gtcatcccgc tgcctgcggt ggaggtcccc
    11221 acacgcatat caccccggga gctgaaggtg cggataacgg aaggctgtac ccaccagttc
    11281 accggcagtt cacgcacact gtgtttggca ctgccgcgca gggtgtcacg ggatgaggtg
    11341 cctttaccaa agttcatatc gttgtggctg cccagacgaa atccggcacg cacatgttgt
    11401 gcactgccat gcccgaactt cacataaccg gcgttgtcct taccgtcatc cagggaaagt
    11461 ccctgccagg tgtactgcag ttgtggctcc agcatcaggt tgtcagtgat actgaagggc
    11521 agaccggttt ccagtgagcc cagccagccc cggccccgtg cgcggaagtc gtgattgtcc
    11581 gatgaggctt tcatgctgtg gcgggttccc tgggccacaa tgtcagccca cagtccggag
    11641 gcgttgtgta tcagattcag gtatccgccc aggctgccgg catcatcccg gaccgtgccg
    11701 gcacgggagc tgtcatcatc cttaacatca acggaagaat ggcctgcagc accatacacc
    11761 cctgtcgtca gagacatacc ggcaacctct gttctcatca ggtcaccctc cag
//
LOCUS       DAZFRN010000147        11757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFRN010000147 DAZFRN010000000
VERSION     DAZFRN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.149"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..281)
                     /gene="yehE"
                     /locus_tag="ACHKUB_003978"
     CDS             complement(<1..281)
                     /gene="yehE"
                     /locus_tag="ACHKUB_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO6656934.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(544..1653)
                     /gene="apbC"
                     /locus_tag="ACHKUB_003979"
     CDS             complement(544..1653)
                     /gene="apbC"
                     /locus_tag="ACHKUB_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO6656935.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1785..3818
                     /gene="metG"
                     /locus_tag="ACHKUB_003980"
     CDS             1785..3818
                     /gene="metG"
                     /locus_tag="ACHKUB_003980"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO6656936.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3959..7753
                     /locus_tag="ACHKUB_003981"
     CDS             3959..7753
                     /locus_tag="ACHKUB_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO6656937.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAVAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLFTIIQEHPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCTQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHACADLLPAIV
                     VSPPWLSKKKKATIPVLELAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTA
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARSWLLKYPEHAITGLLPAALGKADEAQDNARAALRMLSEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     RESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDASGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSTENQLLTCFRVAEDNSYST
                     ADDDLFTLPEGDISVGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGTPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7763..11395
                     /gene="yehI"
                     /locus_tag="ACHKUB_003982"
     CDS             7763..11395
                     /gene="yehI"
                     /locus_tag="ACHKUB_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO6656938.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAVLACIAQ
                     KDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGIPKIRRPFIALLLPEKPEIANELASL
                     ESSRSSLHSKEWLKVIATDNTAVKKLERCWGLDVFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRFIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11456..>11757
                     /gene="yehK"
                     /locus_tag="ACHKUB_003983"
     CDS             11456..>11757
                     /gene="yehK"
                     /locus_tag="ACHKUB_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO6656939.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIF"
BASE COUNT         3066 a         3009 c         2907 g         2775 t
ORIGIN      
        1 taatcgtagc gatttaacac gattcgacgt ttgctatcgc tacctgcaac aaccacttct
       61 gtcgtaacac ctttgacggg cgaagaaacc tgatgacggg tgtcaacatt atacgttaac
      121 tgaccacaat gctgttgtgg ttgactatta accatagcca tactgcatgt tgggggactg
      181 atcctgccat taatcgttaa tgtggcagtc tctgaagaga atgcgggaga agccaatcca
      241 taggcaagaa aaataattcc tgacagccaa tacttattca ttgaacgtta tccctgtagt
      301 aaaggttatg cctggataga atgagtgcat aacaaactat agctgtacat ccactacaca
      361 gccacgaagg atgataatga agcattgcct gtatgatcaa tcgactttgt agagtttcgg
      421 acgaaggtcc gcagaatatt cgcagtatta aataagtgtt cattaatgcg gcaaaaacta
      481 atacaccgca tcaatgtaac atctctgcgg tgtatgaaga ataaccagaa tggttaatag
      541 gcgttagacc gcgcggaagg aaatctcgcc tggaatgact tcaccctgcc agtagagctg
      601 agctgcaacg cggtcagcca gctggcggta gatagcggta aattcgctct ccggacggct
      661 gataaccgtt ggcgtgcctt tatcgagatc ctcacgtaag gaaatgtgta acggcatctg
      721 ccccagcagc tgtgtgtggt atttttcagc cagtttctct gcaccaccgg taccgaaaat
      781 cggttcatgg tgaccacagt tactgcaaat atgcacactc atgttctcga caataccaag
      841 caccggaacc tcgactttct cgaacatcac aatgcctttt ttcgcatcga tcaacgcgat
      901 gtcctgcggg gtggtgacca cgactgcacc agttactgga atgttttgcg ccagcgtcaa
      961 ttgaatgtca ccggtgcctg gcggcatatc aagcaccaga tagtcgagat ccggccacaa
     1021 ggtttcctgc aacatctgca ttaacgcctt gctcgccatc ggtccacgcc acaccatcgc
     1081 attgtcgtca gtgaccagat aaccaattga attggttgcc aggccatgag acataatcgg
     1141 tgccatatgg gtaccgtccg gcgaagttgg acgctgattt tctgcgccca gcatggtggg
     1201 aattgatggg ccgtagatat cggcgtccag aatgccgact ttcgcccctt ccgccgccag
     1261 tgccagcgcc aggtttaccg ccgtggagga tttacccacg ccgcctttac cggaactgac
     1321 cgcaatgatg ttcttcacac cattaatacc cggctgattc ttcacgcgtt ttaaggtggc
     1381 gatattgtgc gacagtttcc agtcgatagc tttcgccccg gtgatacgca gcagttcggc
     1441 gctacattgc tcttttaact cttcaaacgc gctatgccag acgaatggca taaccagttc
     1501 cacatgcaac gtgtcgtcca tccaggcgac atggtgcaac gctttcagcg tggtgaggtt
     1561 atgcttcagg gttgggtgct gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc
     1621 ttctggtgat ttggcctggg attgttcgtt catcccgact ccttttttgt atagataaac
     1681 catcagctga tagtttacct gaagaatata gagaagtact tacttaacat tttcccattt
     1741 ggtactatct aacccctttt cactattaag aagtaatgcc tactatgact caagtcgcga
     1801 agaaaattct ggtgacgtgc gcactgccgt acgctaacgg ctcaatccac ctcggccata
     1861 tgctggagca catccaggct gatgtctggg tccgttacca gcgaatgcgc ggccacgagg
     1921 tcaacttcat ctgcgccgac gatgcccacg gtacaccgat catgctgaaa gctcagcagc
     1981 ttggtatcac cccggagcag atgattggcg aaatgagtca ggagcatcag actgatttcg
     2041 caggctttaa catcagctat gacaactatc actcgacgca cagcgaagag aaccgccagt
     2101 tgtcagaact tatctactct cgcctgaaag agaacggttt tattaaaaac cgcaccatct
     2161 ctcagctgta cgatccggaa aaaggcatgt tcctgccgga ccgttttgtg aaaggcacct
     2221 gcccgaaatg taaatccccg gatcaatacg gcgataactg cgaagtctgc ggcgcgacct
     2281 acagcccgac tgaattgatc gagccgaaat cggtggtttc tggcgctacg ccggtaatgc
     2341 gtgattctga acacttcttc tttgatctgc cctctttcag cgaaatgttg caggcatgga
     2401 cccgcagcgg tgcgttgcag gagcaggtgg caaataaaat gcaggagtgg tttgaatccg
     2461 gcctgcaaca gtgggatatc tcccgcgacg cgccatactt tggttttgaa attccgaacg
     2521 cgccgggcaa atatttctac gtctggctgg acgcaccgat tggctacatg ggttctttca
     2581 agaatctgtg cgacaagcgc ggcgacagcg taagcttcga tgaatactgg aagaaagact
     2641 ccaccgccga gctgtaccac ttcatcggta aagatattgt ttacttccac agcctgttct
     2701 ggcctgccat gctggaaggc agcaacttcc gcaagccgac caacctgttt gttcatggct
     2761 atgtgacggt gaacggcgca aagatgtcca agtctcgcgg cacctttatt aaagccagca
     2821 cctggctgaa tcattttgac gctgacagcc tgcgttacta ctacactgcg aaactctctt
     2881 cgcgcattga tgatatcgat ctcaacctgg aggatttcgt tcagcgtgtg aatgccgata
     2941 tcgtgaacaa agtggttaac ctggcctctc gtaatgcggg ctttatcaac aagcgttttg
     3001 acggcgtgct ggcaagcgaa ttggctgacc cgcagttgta caaaaccttc actgatgccg
     3061 ctgaagtgat tggtgaagcg tgggaaagcc gcgaatttgg taaagcagtg cgcgaaatca
     3121 tggcgctggc tgatctggct aaccgctatg tcgacgagca ggctccgtgg gtggtggcaa
     3181 aacaggaagg ccgcgatgcc gacctgcagg caatttgctc aatgggcata aacctgttcc
     3241 gcgtgctgat gacttacctg aagccggtac tgccgaaact gaccgagcgc gcagaagcat
     3301 tcctcaatac agaactgacc tgggatggta tccagcaacc gctgctgggc cacaaagtga
     3361 atccgttcaa agcgctgtat aaccgcatcg atatgaagca ggttgaagca ctggtggaag
     3421 cgtctaaaga agaagtgaaa gcagctgccg cgccagttac tggcccactg gcagatgatc
     3481 cgattcagga aaccatcacc tttgacgact tcgcaaaagt tgacctgcgc gtggcgctga
     3541 tcgaaaacgc agagtttgtt gaaggttctg acaaactgct gcgcctgaca ctggatctcg
     3601 gcggtgaaaa acgcaatgtc ttctccggta ttcgttctgc ctacccggat ccgcaggcac
     3661 tgatcgggcg tcacaccatt atggtggcca acctggcacc gcgtaaaatg cgctttggta
     3721 tctccgaagg catggtaatg gctgctggtc ctggcgggaa agatatcttc ctgttaagcc
     3781 cggatgctgg tgctaaacca ggccatcagg tgaaataatc cccctttaag gcgctgcatc
     3841 gacagcgcct tttctttata aattcctaaa gttgttttct tgcgattttg tctctctcta
     3901 acccgcataa atactggtag catctgcatt caactggata aaattacagg gatgcagaat
     3961 gagacacttt atctatcagg acgaaaaatc acataaattc tgggcagttg agcaacaggg
     4021 aaacgagttg catatcagtt ggggaaaagt tggcaccaaa gggcaaagcc agataaaaag
     4081 tttttcagat gctgcggcag tggcaaaagc ggagcttaag ctgattgcgg agaaggtgaa
     4141 gaaggggtat gtggagcaag cgaaggataa ttctttgcaa ccttcccaaa cggtaacggg
     4201 ctctctcaag gtagcggact tattcaccat tattcaggaa cacccctctt tcgtagcaga
     4261 aacccgtgcg cctgacaaaa atacagatgc tgttttaccg tggctggcaa aagatattgc
     4321 tgtcgttttt ccgcccgaag ttgtacacac cacgttaagt catcgccgct ttcccggagt
     4381 tcctgttcag caagcagaca aattgaccca attacgtcgc ttagcctgta gtgtgtcgca
     4441 acgggataat aaaacagcca catttgactt cagcgcctgt tctttagaat ggcaaaacac
     4501 cgtcgcccag gcgatcagtc agatcgacgg cctgaaaaca acacagctac catcaccagt
     4561 aatggctgta ctcacggcac ttgaaatgaa atgcacaaga tataaagtgc gtgaggatgt
     4621 tatggatcag atcgtccagg agggcggtct ggaatatgct actgatgtaa taatacacct
     4681 tcaacagatt gatattaaat gggattatgc gaataatgtc attattattc tgccgtctgg
     4741 cattgcacct gactacttgg agcaatattc cagatttgaa ttacgcctac gtaaacattt
     4801 atcactggcg gaagagtctc tctggcaaaa atgtacacaa aaacttattg ccgcaattcc
     4861 acatattcca gaatggcggc aaccattaat tgctttgtta ttacccgaaa aaccagaaat
     4921 tgcacatgaa attgcccagc gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt
     4981 aaaaatagtg gcgactgatg agcacattct tgcctcatta gaaaaatatc acgagccata
     5041 tgccattttt gatgattact attgtggtgc gatatggtca gccaccgtat tacaggagca
     5101 aggtgttgca gccctgcccc gatttgctcc ctatgccgca agtgactact gcgccgatgt
     5161 gttgcgtcat atcaatcatc cgttcgcatt gacactgctt atacgtgtag ccgggcaaac
     5221 taaacgctgt cacgatcgga tgacgaaagc cattgctgcg ttcccacatg cagcaatggc
     5281 ggcactgacg gaacttcttg ggcaaaaaga agagaacagt tggcgcatta tgctaatgac
     5341 aatgcttatc tcacaaccag cactggcaga acaggtcatt ccctggctct cgacacccgc
     5401 agttgccgta ctgaaatcat gccagcaaca actgacacag ccctcaaacc atgcctgcgc
     5461 cgatctactg cctgccatag tagtctcccc accatggctt tcgaaaaaga aaaaagcgac
     5521 gattccggtg ctggagttag cgccattagg cattgagcca atctgttatc tgacagaaga
     5581 aatcagtaat caacttttgg cgaaatatat ctggtattca aaacacatca cggttagcca
     5641 tgaagaaagt actgccaacc tgttggcaag aatgggtttt caacgacgga tcgctggtac
     5701 atatattaaa gctcccgaag cggtagttga ggcatggcta aatgaagatt attcaacctt
     5761 actaagtgaa tttaaggtgt ttcattcacc taccgggcat tattggcagt tggggatttt
     5821 gacaacattg ccgctggaga aagcagtaaa agcatggaat gcccttaccc tatctccaca
     5881 taccgatacc gaatacgcca tgttacattt tggactcaaa gggttacctg ggttagtaaa
     5941 ctcacttgca cgctatccac aggaagcctt gcccatcacg aattacttcg cagcgagtga
     6001 gctggcacct gccgtcgccc gcgccttcaa caaactcaaa actctacgac aagacgcccg
     6061 tagctggctg ctgaaatacc cggaacacgc cattaccggc ctgctgcctg ctgcgctcgg
     6121 caaagccgat gaagctcagg ataacgcccg cgctgccttg cgtatgctta gcgaaaacgg
     6181 tcatcagcca ttactgcaag aaatcgcccg gcgttataac cagccggaag taaccgacgc
     6241 ggtgaacgct ctgcttgcgc tcgatccctt agataatcac ccgacaaaaa tccccactct
     6301 tccggccttt tatcagccat cgctctggac gcgcccggta ttaaaagcaa acgcccaatc
     6361 actgccagat agcgccctcc tccacctcgg cgaaatgctc cgcttccctc aggaagaggc
     6421 tctgtatccg ggattattgc aggtgaaaga tgcctgtacc gccgactcac tggctgaatt
     6481 tgcctgggat ctgtttaccg cctggcagac cgctggcgcg ccgtcgagag agagttgggc
     6541 gttcactgcg ttaggcgttc tcggtaacga tgacaccgcc cgcaaactga cgccattaat
     6601 ccgcgcctgg cctggcgaat ctcagcataa acgcgccacc gttgggttgg atattctcgc
     6661 cgctatcggt agtgatatag ccctgatgca gcttaacggc attgcccaga aactgaaatt
     6721 caaagcatta caggagcggg cgaaagagaa aattgccgac attgccgaaa gtcgcgaact
     6781 gacagtggcg gagcttgaag atcggttagc accagatctc ggtctggatg ataacggttc
     6841 gttgctgctg gatttcggcc cacgccagtt caccgtcagc tttgatgaaa ccttaaaacc
     6901 gtttgtgcgt gacgcttccg gcagccgtct gaaagacctg cccaaaccaa acaaaagcga
     6961 tgatgaatca caggcgaacg atgctgttaa ccgctacaaa ctgctgaaaa aagatgcgcg
     7021 taccgtcgcc gcccagcagg tagcaaggct ggaatccgcc atgtgcctgc gtcgccgctg
     7081 gtcgccagaa aactttcagc tcttcctggt tgaacatcca ctggtacgcc acttaacccg
     7141 ccgtctgatt tggggcgttt atagcaccga aaaccagcta ctgacgtgct tccgcgtggc
     7201 ggaagataac agctacagca ccgctgacga tgatcttttc accctgccgg aaggcgatat
     7261 ctctgttggc attcctcacg ttctggaaat atcaccgacg gatgctgccg cctttggtca
     7321 gctttttgcc gactacgaac tgctaccacc gttccgtcag cttgaccgta acagttacgc
     7381 cctgacagaa gccgagcgca acgccagcga actgacccgc tgggcaggca gaaaatgccc
     7441 gagcggtcgg gtcatggggc tggcgaataa aggctggata aagggcaccc cgcaggatgg
     7501 aggctggatc ggctggatga tcaaacctct cggtcgctgg tcgttaatca tggaaatcga
     7561 cgaaggtttt gcggtaggca tgtcgccagc cgaactgagt gccgagcagc tcttaagcaa
     7621 gctgtggcta tgggaaggca aagcagaaag ctatggctgg gggagtaatt caacacagga
     7681 agcgcagttc tccgtactcg atgccatcac cgccagcgag ctaattaacg atattgaagc
     7741 cctgtttgaa taaggaaaga gcatggacaa ggaattaccg tggctggcgg ataacgctca
     7801 actggaactg aaatataaaa aaggcaaaac accactcagc catcgcaatt ggccgggcga
     7861 accggtgcct gttatcactg aaagcatcat tcagacactg ggtgatgaat tgctacaaaa
     7921 agctgagaag aaaaaaaaca ttgtctggcg ttatgaaaat ttttcactgg agtggcagtc
     7981 cgccattacg caggccatca acttgatcgg cgaacacaaa ccctcagtcc cggcccgcac
     8041 aatggcggtg ctagcctgta tcgcgcaaaa ggacagccaa cagttgctcg acgaaatcgt
     8101 ccaacaagag gggctggaat atgcgactga ggtggtgatt gcacgccagt ttattacacg
     8161 atgttatgag agtgatcctc tgctagtgac attgcagtat caggacgagg attatggcta
     8221 tggttatcgc tcagaaacct ataacgaatt cgatctccga ctgcgtaagc atctctctct
     8281 ggcagaggaa agcagctggc agcgttgcgc cgacaaactc attgccgcac tgccaggaat
     8341 acccaaaatt cgccgtcctt ttattgcact actcctcccg gaaaaacctg aaatagccaa
     8401 tgaactcgcc agtcttgaaa gctcccggtc aagtctccat tcaaaggagt ggttaaaggt
     8461 cattgctact gataatacgg cggtaaaaaa actcgaacga tgctggggcc tggacgtatt
     8521 tagcgatcga gaagccagct acatgtcgca tgaaaaccac ttcggctacg cggcctgcgc
     8581 cgcccttttg cgcgaacaag gactggcagc cgttccgcgc ctcgcgatat atgcccataa
     8641 agaagattgc ggcagtctgc tggtacaaat taaccatccg caagtcatcc gcaccttgct
     8701 gttagttgct gataaaaaca aacccagcct gcaacgtgtg gctaaataca gtaaaaactt
     8761 cccccatgcg acactcgccg cactggcaga actgctggcg ttaaaagaac caccagcccg
     8821 cccaggttat ccaatcatcg aagacaaaaa gctgcctgca cagcaaaaag cacgcgatga
     8881 atactggcgt acgctgttac aaacgctgat agcatcgcag ccacaactag cagaaagggt
     8941 gatgccgtgg ttaagtactc aagccagggc agtgctgaat agttatttat cggcaccgcc
     9001 aaaaccggtt attgatagca ccgataacag cagtctgcct gaaatgctcg tttcaccacc
     9061 gtggcgtagt aagaaaaaaa tgacagctcc acgtcttgat ttggcaccgc tcgaattaac
     9121 tccgcaaatt tactggcaac caggcgaaca agagaggctt gccgccactg agtctgcccg
     9181 ttatttcagc acggaatctc ttgcggaacg catggaacaa aaaagtggac gagttgtatt
     9241 acaggaactg ggttttgggg atgatgtatg gctgtttctg aattatatac tccccggaaa
     9301 actggatgct gcacgcaatt cactcattgt tcagtggcat tactaccagg ggcgggttga
     9361 agagatcctg aatggctgga actccccgca agcacaatta gcagaacagg cgctccgcag
     9421 cggtcacata gaagcgttaa ttaacatatg ggaaaatgac aacttctcac gttatcgtcc
     9481 ggaaaagagt gtctggaacc tgtatttatt ggcacagttg ccgcgtgaga tggctttgac
     9541 cttctggctg cgtatcattg agaaaaagca tctgttcgcg ggtgaggact attttctcag
     9601 tatcctcgga ttggatgcgc taccaggtct gctgttggct ttttcacatc gtccaaaaga
     9661 aacatttccg ttaattttaa attttggcgc aacagaactg gcgctgcctg ttgcccgcgt
     9721 ctggcgacgt ttcgcggcgc agcgtgatct ggctcgccag tggattttac aatggccgga
     9781 acatacggct agtgcactta tccctcttgt ctttaccaaa cccagcgata atagcgaagc
     9841 cgcattactt gccctgcgtt tactgtacga acagggacat ggcgaattgc tacaaaccgt
     9901 ggcaaaccgc tggcagcgta cagatgtatg gtctgccctg gagcagttgc ttaaacaggg
     9961 tccaatggac atttacccgg cacgcattcc aaaagcccct gatttctggc atccggcaat
    10021 gtggtccagg cctcgactta tcactaataa tcagcctgtt accggtgacg ctctggaaat
    10081 tatcggcgaa atgctgcgct ttacccaggg gggacgtttt tatagcgggc tggaacaact
    10141 gaaaacgttc tgccagccac aaacgctggc agcttttgcc tgggatctct tcactgcgtg
    10201 gcaacaagct ggtgcccccg caaaagacaa ctgggcattt ctggcgttaa gtctctttgg
    10261 tgacgaaagc acggcacggg atctgacgac acagatcctc gcctggccac aagaaggcaa
    10321 atctgcccgt gctgtcagcg gcctgaacat ccttaccctg atgaataatg atatggcgct
    10381 gatacagctg catcatatat cgcaacgggc taaatcccgc cccttacgtg ataacgcggc
    10441 ggaatttctt caggtggtcg cagaaaatcg cgggctaagc caggaagagc tagcggacag
    10501 attagtccca accctgggcc ttgatgatcc gcaggcgttg agttttgatt ttggtccccg
    10561 gcagtttacc gttcgcttcg atgaaaacct caacccggtt atctttgatc agcaaaacgt
    10621 tcgccagaaa agcgttcccc ggttgcgcgc cgatgacgat caactgaaag cgcccgaggc
    10681 actggcccga ctaaaagggc taaaaaaaga tgctactcag gtgagcaaaa acctgctccc
    10741 gcgtcttgaa gctgccctac gtaccacccg acgctggtcg ctggcagatt ttcatactct
    10801 gtttgttaat catcccttta cccgcctggt tacccagcga ttcatatggg gcgtgtatcc
    10861 ggcaaatgaa ccgcgtcgtt tactcaacgc ctttcgtgtg gccgcagagg gggagttctg
    10921 caatgcgcag gatgagccaa ttgacctgcc tgcggatgct ctgattggca ttgcccaccc
    10981 gttagaaatg acagcagaaa tgcgcagtga atttgcccag ctttttgccg attacgaaat
    11041 tataccgcca cttcgccagt tgacacggcg cacggtgctg ctcacgcctg acgagtcagc
    11101 cagtaatagt ttgaatcgct gggaaggtaa atctgctacc gttgggcaac ttatgggaat
    11161 gcgatacaaa ggctgggagt caggctatga ggacgcattt gtctatgacc tgggtgagta
    11221 ccggctggtc cttaagtttt cacccggttt taaccactac aatgttgata gcaaagcgct
    11281 aatgagcttc cgttctcttc gagtgtacag tgacaataaa tccgtcactt ttgccgaact
    11341 tgatgtgttt gatttgagtg aggcgttaag cgcacctgac gtcattttcc attaaaacac
    11401 agcgggcagt gatgcaactg cccgttatca acacgactta ccagcgggga aagcgatgat
    11461 cgtgcaaaaa gagctggtcg ctatttacga ttatgaggtc cctgtacctg aagatccgtt
    11521 ttccttcaga cttgagatcc ataaatgctc tgaattattt acaggttccg tctatcgact
    11581 ggagcgattc cggctacgtc caacatttca tcaacgtgat cgagaagatg ctgacccgct
    11641 aataaatgat gcgttgattt atataagaga tgagtgtatt gatgagcgga aattacgagg
    11701 tgaatcacct gaaactgtaa tagcaatttt taatcgtgaa ctacagaata tattcaa
//
LOCUS       DAZFRN010000148        11583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFRN010000148 DAZFRN010000000
VERSION     DAZFRN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.150"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(145..897)
                     /gene="tcyN"
                     /locus_tag="ACHKUB_003984"
     CDS             complement(145..897)
                     /gene="tcyN"
                     /locus_tag="ACHKUB_003984"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO6656940.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MHPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(894..1562)
                     /gene="tcyL"
                     /locus_tag="ACHKUB_003985"
     CDS             complement(894..1562)
                     /gene="tcyL"
                     /locus_tag="ACHKUB_003985"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO6656941.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1577..2563)
                     /gene="dcyD"
                     /locus_tag="ACHKUB_003986"
     CDS             complement(1577..2563)
                     /gene="dcyD"
                     /locus_tag="ACHKUB_003986"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO6656942.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2668..3468)
                     /gene="tcyJ"
                     /locus_tag="ACHKUB_003987"
     CDS             complement(2668..3468)
                     /gene="tcyJ"
                     /locus_tag="ACHKUB_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6656943.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3556..4107)
                     /gene="fliZ"
                     /locus_tag="ACHKUB_003988"
     CDS             complement(3556..4107)
                     /gene="fliZ"
                     /locus_tag="ACHKUB_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO6656944.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4153..4872)
                     /gene="fliA"
                     /locus_tag="ACHKUB_003989"
     CDS             complement(4153..4872)
                     /gene="fliA"
                     /locus_tag="ACHKUB_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO6656945.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5192..7024)
                     /locus_tag="ACHKUB_003990"
     CDS             complement(5192..7024)
                     /locus_tag="ACHKUB_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO6656946.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSTSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSDTLNLAGFNVNGKGSVANTAATTDNLTLAGFTAGTKAADGTVTYSKNVQFAAA
                     TASNVLAAAKDGDEITFAGNNGTGIAATGGTYTYHKDSNSYSFSATAASKDSLLSTLA
                     PNAGDTFTAKVTIGSKSQEVNVSKDGTITSSDGKALYLDEKGNLTQTGSGTTKAATWD
                     NLMANTDTTGKDAYGNSAAAAVGTVIEAKGMTITSAGGNAQVLKDAAYNAAYATSITT
                     GTPGDAGAAATAGNAAVGALGATAVDNTTADVADISISASQMASILQDKDFTLSDGSD
                     TYNVTSNAVTINGKAANIDDSGAITDQTSKVVNYFAHTNGSVTNDTGSTIYATEDGSL
                     TTDAATKAETTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQS
                     RIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7290..8696
                     /gene="fliD"
                     /locus_tag="ACHKUB_003991"
     CDS             7290..8696
                     /gene="fliD"
                     /locus_tag="ACHKUB_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6656947.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNSIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8721..9131
                     /gene="fliS"
                     /locus_tag="ACHKUB_003992"
     CDS             8721..9131
                     /gene="fliS"
                     /locus_tag="ACHKUB_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6656948.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            9131..9496
                     /gene="fliT"
                     /locus_tag="ACHKUB_003993"
     CDS             9131..9496
                     /gene="fliT"
                     /locus_tag="ACHKUB_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO6656949.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            9574..11061
                     /gene="amyA"
                     /locus_tag="ACHKUB_003994"
     CDS             9574..11061
                     /gene="amyA"
                     /locus_tag="ACHKUB_003994"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6656950.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WVRWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(11095..11508)
                     /gene="yedD"
                     /locus_tag="ACHKUB_003995"
     CDS             complement(11095..11508)
                     /gene="yedD"
                     /locus_tag="ACHKUB_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO6656951.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         2839 a         3024 c         2869 g         2851 t
ORIGIN      
        1 attattcagg caatgaatta cttttgcaag ccatcgcatt ctcttatgtt attaatgagt
       61 tatgctgatt tgttaagcag ttttatcacg cttgaaatgg cgtcccgccc cgacaggtga
      121 atcgtcgggg ctgatttttt cttattattg cagcagaaac ttctcgagga actggcgggt
      181 gcgaggctgc tggggatcag caaataacgc ttttgctgcc ccctgctcga ctatccgccc
      241 ctggtccata aaaatcgccc ggtccgcaac atcccgggca aagctcattt cgtgcgtcac
      301 aatcaccatc gtgcgctttt cctgcgccag ctggcggatt gtgttcagga cttcgcccac
      361 cagctctgga tccagcgctg acgttggctc gtcaaacaaa atcacctcag gatgcattgc
      421 cagcgcacgc gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac gtggatagct
      481 ggtttcttta ccggccagcc caactttcgc cagcagctcg cgagcgcgcg ccgtggcctc
      541 ttctttcggt tcgcctttga cgatcaccgg cccttcaata atgttttcca gcaccgtacg
      601 atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca actggcgaat
      661 cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc caacggtgat
      721 cgttcccgct tccggttgtt ccagcagatt tatgctgcgt agcaacgtgg ttttgccgga
      781 accactcgga ccgataattg ccaccacttc gccaggcttt acctcgaggt cgataccgtg
      841 cagcaccgtc tgaccgtgaa attttttcac caggttctta acttcaatgg cactcatttt
      901 ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt cgataatact
      961 gtcgccatga tccagtagat gagcgaagcc gccaggtaca tggtgaacac ctccagcgtg
     1021 cgcgaggtaa tcaactgcgc ctgacggaac agctccggca cctggattgt cgcggccagc
     1081 gacgtatctt ttaccagact gataaagctg ttcgacagcg gcggcaacgc cacccgcgcc
     1141 gcctgtggca aaatcgcgcg acgcatggtc tgccacggcg tcataccaat actggcagcg
     1201 gcttcccact gacctttatc aatagaagaa atggcggcgc gcagcgtttc ggcggcatag
     1261 gcggcagtgt tcagcgacag accaatcatt gctgacggaa tgggatctaa ttcgatacca
     1321 aactgcggca ggccgtaata gatcataaac agttgtgcga tgagcggcgt accacgaaaa
     1381 atagagatat aaaaacgcgc cagccagcgc acgggccaga ttggcgacag gcgcatcagc
     1441 gcgagaataa acccgagcag taagccaaaa aacatgccgc cgatactgag ttgcagcgta
     1501 tacccagccc ctttcaacag gaacggcaag gaatcaataa ccagttgtat actttcttgc
     1561 atgagcgttc ccggagctaa acgtggggat gataggcgaa cagcgcaggc gcaccgccgg
     1621 tatgaataaa cagaatcggc ccttcatctt tgaagcgttt ctgactgata ccgccaatca
     1681 gccccgccat cgcttttccg gtatacacag gatcaagcag aatgccttcc agccgcgcca
     1741 gcaatttcac tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt
     1801 catcccagag taaaatttcc gctgatgcgg tcagctccag ttctttcgca atcgcctgtt
     1861 gtaggttaac cactttcggc aactgatcgg caacggaacg cgacacggtc acgccaatca
     1921 gttcgctttc aggcatcagg tgttccagcc caacagccag tccggcgtga gttccggcac
     1981 tgcccgatgc gactaccacc gacgaaatat taaccgcccc ttcacactgt tgcgcgattt
     2041 ccagcgcact ctccacataa cctagcgcgc caagagcatt agaaccgcca accggaatga
     2101 catacgggcg aaagccttgt gcttcgactc gcgtcgccag ctcttccagt tgggcattgg
     2161 gatcggtcag tgcatcgcac atttcaatct gggtattgaa cagatccagc aacaaacgat
     2221 tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc agcgccacgc
     2281 agtgcagacc gagtttcgcc gcgactgcgg cagtctggcg cacatggtta gactggatcg
     2341 cgccggcagt aatcagcgta tcggcacctt cgcgcagggc atctgccgcg agaaattcca
     2401 gcttacgtaa tttattgccg cccattgcca tgggtgtgac gtcatcccgt ttgatgaaaa
     2461 tttcccgtcc aagataatca gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga
     2521 taaactccaa ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc tccgatgtgt
     2581 gttgttgtga ttttcttatt atgcacgctg aaaacgcgta aataaaaaag gcgctagtga
     2641 aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa accatttttc
     2701 ggaaagagct tgcagagtgc cgtctttttg catttccgca attgcatcat tcaccgcttt
     2761 cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg agaatgcttc
     2821 accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg ccagacgatc
     2881 aacgaggatc gcatcgatac ggcctacgcg cagatcctga tatttggtcg ggtcatcatc
     2941 ataggtacgc acatcgacgc cctgaacatt ctgccgcagc cactcttcat agttggtgcc
     3001 cagaccgaca cccacttttt tgcctttcag atcagcagcc gttttaatgg tgccttcgtt
     3061 accttttttc accagcgcct gaataccgga aatggtgtaa ggggtagaga aatcgtattt
     3121 tttcttgcgc tcgtcagaaa tggtgacctg attaatcacc acatcaatgc gtttagagtc
     3181 cagcgacgcc agcataccgt cccatttggt cggttttagt gacgcctcaa cgccaagatg
     3241 ttttgccagc tgttgggcaa attccacttc aaaaccggtt aatttgccgt catccccctg
     3301 aaaactgaac ggcggataag ttccttccag ccctaccagc agcgtgccgc gctctttgac
     3361 tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa ccagcgccac
     3421 ggccatcaca cccatcaatg cctgacgtcc cagatgtgct aatttcatat tcaccccgaa
     3481 tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa caactccgct
     3541 acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt tctgcacaag
     3601 tccggtacac gtttgacgct gatagtgttg atacttccgt aatgcgatgc ggtaattgtt
     3661 ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt cctgcagctg
     3721 atcgagggaa atattttgct cgtgtagatg attccccaaa cggcgcagac gaacgacata
     3781 ttcacgcacc gtacctgggc tcatttcagt ttgctcaaaa agaaattgct taaagccgat
     3841 aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc ggcacaatgc
     3901 cgcccatgat tgttgttcca cttgccagcc attttcatca agcagcgtgt ccaggcggga
     3961 aatctcgatt ttattcacta ttttgccgtc gcgaactaag gtaatgcgat cgagtaattt
     4021 acggcaatgc gcgcaatggg tctggctgtg tttaaagtct ttaagatagc ggcttaatgg
     4081 ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaaat taaagtgcgg
     4141 catttactga cgttataact tacccagttt agtgcgtaac cgtttaatgg cctggctgtg
     4201 taactgactg acccgcgatt ccccgacctc cagcaccgcg ccaatctctt tgagattcag
     4261 ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg tttcgatagc
     4321 ttccatcact cgctggcgca gattactgtc cagcagttgt tgtagcgggt tttctcgctg
     4381 atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcgcgccact catcgtagga
     4441 gaagagctgg ctgttattgg tgtcgagcaa catttggcga taatcggcaa tatcgatccc
     4501 taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct ccagttgccc
     4561 tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc agtcacggct
     4621 gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg taaatgccgt
     4681 tccttgtagg gcgtcatagc gttcgacggc attaagtaac ccaatgccgc ccgcctgtag
     4741 cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgacg
     4801 caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt cagcggtata
     4861 gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat tatccgtttc
     4921 tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg gggttaccgg
     4981 taaattccat gcagaaaaaa acccgccggt ggcgggggag cacgttgctg acaaattgcg
     5041 ctttatgttg ccggatgcgg cgtaaacgcc ttatccggcc tacaaaaatg tgcaaattca
     5101 ataaattgca attccccttg taggcctgat aagcgcagcg catcaggcaa tttggcgttg
     5161 ccgtcagtct cagttaatca ggttacgacg attaaccctg cagcagagac agaacctgct
     5221 gcggtacctg gttagctttt gccagcacgg agttaccggc ctgctggatg atctgcgctt
     5281 tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg aatacgggac tgcgcttcgg
     5341 acaggttggt agtggtgttg ttcaggttgg tgaccgcgga atccagacgg ttttgcaccg
     5401 caccgaggga ggagcggaat ttgtcgatgg agctgatggc ttcgtccaga gctttcaggg
     5461 gatcggcggt ggtttcggct ttggttgctg catcggtggt caggctacca tcttctgtcg
     5521 cataaatagt ggagcctgta tcgttagtca cgctaccgtt agtatgagcg aaataattga
     5581 caactttact ggtttggtct gtgattgcgc cgctgtcatc aatgtttgct gctttgccat
     5641 tgatagtgac agcattgctg gtcacgttgt aagtatcact accatcactt aaggtgaaat
     5701 ctttatcctg aaggatgctc gccatttgcg aagctgagat agagatatcg gcaacatctg
     5761 ccgtggtatt atcaactgcc gttgcgccca gcgctcccac cgcggcatta cccgcagttg
     5821 cagcggctcc cgcatcaccc ggagtaccag tagtaattga ggtcgcatat gcggcattat
     5881 aagccgcgtc ttttaacacc tgagcattac caccagcaga agtgatggtc attccttttg
     5941 cttcgattac tgtcccaaca gctgctgccg cagagttacc ataggcatct ttgcctgtag
     6001 tatctgtatt ggccatcagg ttatcccagg ttgcagcttt ggttgtgcca ctacctgttt
     6061 gggtcaggtt gcccttctca tctaaataca gcgccttacc atcgctggat gtaatcgtac
     6121 catctttgct aacgttaact tcttgcgatt tagaaccaat agtcacttta gcggtaaatg
     6181 tatcgccagc gtttggtgcc agtgtgctca acagagaatc tttagatgca gccgttgcgc
     6241 taaagctgta tgagttagag tccttatgat aagtataagt ccccccagtt gcagctatac
     6301 ctgtgccgtt attaccagcg aacgtaattt cgtcgccatc tttagcagca gccagtacat
     6361 tgcttgcagt cgcggcggca aactggacat ttttgctata agttacggtg ccatcagcag
     6421 ctttagtacc cgctgtaaaa ccagccaatg tcagattatc tgtagtcgca gcggtattcg
     6481 ctacagaacc tttaccgtta acgttaaaac cagccagatt cagcgtatca gagtcaattt
     6541 tcttcagatc aatagtaatc gtttcgccat cgttcgcgcc gacctgaatt ttcatcgagc
     6601 catctttaga cagcacgttc acgccgttga actgggtctg accagatacg cggtcaattt
     6661 cgtcaagacg ggatttgatt tcgtcctgga tggagtccag gtcagaggtg gagttagttc
     6721 cggtcgtcgc ctgaaccgtc agttcacgaa tacgctgtaa gttgttgtta atttcggaca
     6781 gcgcgccttc agtggtctgc gcaacagaaa taccgtcgtt ggcgttacgt gcagcctgag
     6841 tcaggccttt aatgttagaa gtaaaacggt tagcaatcgc ctgacctgcg gcgtcatcct
     6901 tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat agaactcgac agcgcagact
     6961 ggttcttgtt gatattattt tgagtgatca gcgagaggct gttggtatta atgacttgtg
     7021 ccatgattcg ttatcctata ttgcaagtcg ttgattacgt attgggtttc cacccgtcgg
     7081 ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca acctttagaa tttttttcac
     7141 aaacagccat tttttgttag tcgccgaaat actcttttct ctgcccctta ttcccgctat
     7201 taaaaaaaac aattaaacgt aaactttgcg caattcaggc cgataacccc ggtattcgtt
     7261 ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat ttcatcgctg ggagtcgggt
     7321 caggtctgga tttaagttca atccttgata gcctcaccgc cgcgcaaaaa gcgacgctaa
     7381 cccctatttc aaatcagcaa tcgtcgttta ccgctaaact tagcgcctac ggtacgctga
     7441 aaagcgcgct gacgactttc cagaccgcca atactgcatt gtctaaagcc gatctttttt
     7501 ccgccaccag cactaccagc agcaccaccg cgttcagtgc caccaccgcg ggtaactcca
     7561 tcgccgggaa atacaccatc agcgtcaccc atctggcgca ggcgcaaacc ctgaccacgc
     7621 gcaccaccag agacgatacg aaaacggcga tcgccaccag cgacagtaaa ctcaccattc
     7681 aacaaggcgg cgacaaagat ccgattacca ttgatatcag cgcggctaac tcgtcgttaa
     7741 gcgggatccg tgatgccatc aacaatgcaa aagcaggcgt aagcgcaagc atcattaacg
     7801 tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga caccggcctt gataatgcga
     7861 tgacactctc ggtcagcggt gatgatgcgc tacaaagttt tatgggctat gacgccagtg
     7921 ccagcagcaa cggtatggaa gtctcggtcg ccgcccagaa tgcgcagctg acggtcaaca
     7981 acgtcgccat tgagaacagc agcaacacca tcagcgacgc gctggaaaac atcaccctga
     8041 acctgaacga tgtcaccacg ggcaaccaga cgctaaccat cactcaggac acctccaaag
     8101 cgcaaacggc gattaaagac tgggtgaatg cctacaactc gctaatagat accttcagca
     8161 gcctgaccaa atacaccgcc gtagatgcgg gagctgatag ccagagttct agcaatggcg
     8221 cactgctcgg cgactccacg ctgcggacga ttcagacgca gttgaagtcg atgctgagta
     8281 ataccgttag ttcttccaac tataaaacgt tggcgcagat tggtatcacg accgatccca
     8341 gcgatggcaa actggaactg gatgccgaca aactcaccgc tgcactgaaa aaagatgcca
     8401 gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac cggcatcacg accaccatcg
     8461 gcagcaacct gaccagttgg ctttcgacaa cgggcattat taaagccgct accgatggcg
     8521 ttagtaagac cctgaataaa ttaactaaag actacaacgc cgccagcgat cgcattgatg
     8581 cgcaggtcgc gcgctataaa gaacaattta cccaactgga cgttttaatg acctcgttga
     8641 acagcaccag cagctactta acgcagcagt tcgaaaacaa cagtaattcc aagtaatcaa
     8701 tattcatcgg gagacaggtc atgtacgcgg caaaaggcac ccaggcctat gcacaaattg
     8761 gcgtcgaaag cgccgtaatg agcgccagcc agcagcagct ggtcaccatg ctatttgatg
     8821 gagtgctgag cgcactggtt agagcgcgcc tgtttatgca ggacaacaat cagcaaggca
     8881 aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa cggactgcgg gtgagtcttg
     8941 atgaagagag caaagacgaa ttaacccaaa acttgattgc tctttatagc tatatggtca
     9001 ggcgcttgct gcaagccaat ttacgcaacg atgtctccgc agtcgaagaa gtggaagcat
     9061 taatgcgtaa tattgccgat gcctggaaag agtcgttact ctccccttct ttgattcagg
     9121 acccagtctg atgaaccatg caccgcattt gtatttcgcc tggcaacaac tcgtcgaaaa
     9181 aagccagctc atgttacgcc tggcaacgga agaacaatgg gacgaactca tcgccagcga
     9241 aatggcgtat gtgaatgcgg tgcaggagat agcacacctg actgaagagg ttgccccgtc
     9301 caccacgatg caggagcagc tccgcccgat gctgcgcctg attctcgaca acgaaagcaa
     9361 ggtaaagcag ttattacaga ttcggatgga tgaactggcg aaactggtcg gtcagtcatc
     9421 ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc ggctttgtgc tggctccgca
     9481 agataacttc tcgtgaatct taacaagcta atggctcgcg aataatctga ttacggctac
     9541 gcttctaatg ttccccttga atggagtcga agaatgcgta atcccacgct gttacaatgt
     9601 tttcactggt attacccgga aggcggtaag ctctggcctg aactggccga gcgcgccgac
     9661 ggttttaatg atattggtat caatatggtc tggttgccgc ccgcctataa aggcgcatcg
     9721 ggcgggtatt cggtcggcta cgactcctat gatttatttg atttaggtga gtttgatcag
     9781 aaaggcagca tccctactaa atatggcgat aaagcacaac tgctggccgc cattgacgcc
     9841 ctgaaacgta atgacattgc ggtgctgttg gacgtggtag tcaaccacaa aatgggcgcg
     9901 gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg atgaccgtac gcaaattgac
     9961 gaagaaatca ttgagtgtga aggctggacg cgttacacct tccccgctcg tgccgggcaa
    10021 tactcgcagt ttatctggga tttcaaatgt tttagcggta tcgaccatat cgaaaaccct
    10081 gatgaagatg gcatatttaa aattgttaac gactacaccg gcgaaggctg gaacgatcag
    10141 gttgatgatg aattaggtaa tttcgattat ctgatgggcg agaatatcga tttccgcaat
    10201 catgccgtga cggaagagat taaatactgg gtgcgctggg tgatggaaca aacgcaatgc
    10261 gacggttttc gtcttgatgc ggtcaaacat attccggcgt ggttttataa agagtggatc
    10321 gaacacgtac aggaagttgc gccaaagccg ctgtttattg tggcggagta ctggtcgcat
    10381 gaagtcgata aactgcaaac gtatattgat caggtggaag gcaaaaccat gctgttcgat
    10441 gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg ggcgcgacta cgacatgacg
    10501 cagattttca cgggtacatt agtggaagcc gatcctttcc acgccgtgac gctcgttgcc
    10561 aatcacgaca cccaaccgtt gcaagccctc gaagcgccgg tcgaaccgtg gtttaaaccg
    10621 ctggcgtatg ctctaatttt gttgcgggaa aatggcgttc cttcggtgtt ctatccggac
    10681 ctctacggtg cgcattacga agatgtcggc ggtgacgggc aaacctatcc aatagatatg
    10741 ccaataatcg aacagcttga tgagttaatt ctcgcccgtc agcgtttcgc ccacggtgta
    10801 cagacgttat ttttcgacca tccgaactgc attgccttta gccgcagtgg caccgacgaa
    10861 tatcccggct gcgtggtggt catgtcgaac ggggatgatg gcgaaaaaac cattcatctg
    10921 ggagagaatt acggcaataa aacctggcgt gattttctgg ggaaccggca agagagcgta
    10981 gtaaccgacg aaaacggcga agcgaccttc ttttgcaacg gcggcagcgt cagcgtgtgg
    11041 gttatcgaag aggtgattta aattcatccc cggcggcaag ccggggagat ttcattacgg
    11101 cagtggcgtc ggtaacggcg ctttatccag cgcagcggca cactctgccg tcgggcgatc
    11161 cacacgctcc atcgtcatac catcgtactc aatggtgtta ccgtcacgct caacctcgta
    11221 cagctcacgt tttacggtaa cgttagtgag atcatctgac atcaatgtca acttgcccgg
    11281 cacggcaatc acccgctgcc actgacggca atccagggta tcgccctctt tagtgacaat
    11341 caggctgcct atcgcttccg gactgaccag cgcacgttgc ggccctttgg tctgccagta
    11401 gcccgccagc cagtccggcg cgggggtttt cacgacattg ttatagtttt cgacttcggc
    11461 acagcccgcc agcaacatga gcgcgcctgc aattgcgagt tttttcatca ttcgtcctgc
    11521 atgagaagaa aagaggattg tggcattaaa gccctgatgt cgccagcctt tccaggttca
    11581 gag
//
LOCUS       DAZFRN010000149        11530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFRN010000149 DAZFRN010000000
VERSION     DAZFRN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.151"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(38..1402)
                     /gene="rhlE"
                     /locus_tag="ACHKUB_003996"
     CDS             complement(38..1402)
                     /gene="rhlE"
                     /locus_tag="ACHKUB_003996"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO6656952.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            1631..2302
                     /gene="cecR"
                     /locus_tag="ACHKUB_003997"
     CDS             1631..2302
                     /gene="cecR"
                     /locus_tag="ACHKUB_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO6656953.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2305..3300
                     /gene="hlyD"
                     /locus_tag="ACHKUB_003998"
     CDS             2305..3300
                     /gene="hlyD"
                     /locus_tag="ACHKUB_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO6656954.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            3293..5029
                     /locus_tag="ACHKUB_003999"
     CDS             3293..5029
                     /locus_tag="ACHKUB_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6656955.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5022..6155
                     /locus_tag="ACHKUB_004000"
     CDS             5022..6155
                     /locus_tag="ACHKUB_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6656956.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6166..7272
                     /locus_tag="ACHKUB_004001"
     CDS             6166..7272
                     /locus_tag="ACHKUB_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6656957.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7234..7644)
                     /locus_tag="ACHKUB_004002"
     CDS             complement(7234..7644)
                     /locus_tag="ACHKUB_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO6656958.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7777..8538
                     /locus_tag="ACHKUB_004003"
     CDS             7777..8538
                     /locus_tag="ACHKUB_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6656959.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8535..9776
                     /gene="clsB"
                     /locus_tag="ACHKUB_004004"
     CDS             8535..9776
                     /gene="clsB"
                     /locus_tag="ACHKUB_004004"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO6656960.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9776..10732
                     /locus_tag="ACHKUB_004005"
     CDS             9776..10732
                     /locus_tag="ACHKUB_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO6656961.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10768..11481)
                     /locus_tag="ACHKUB_004006"
     CDS             complement(10768..11481)
                     /locus_tag="ACHKUB_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO6656962.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         2668 a         3061 c         3117 g         2684 t
ORIGIN      
        1 catcagcctg atgccgggca tagcccggca taaaagatta ctgcgcagcg gtaggtttac
       61 gcggacggcg gcgacgttgt tgttcgcctg ccggtttggc atcgccgagg cggcgagacg
      121 gtttttctgc aggtttcgcg cttgcagatt ttgcgccacc ttccccacgg cgtggttgct
      181 gttgaccgcg accaccacct tgcccacgac cgccgccgcc acgttgctgg cgaccgttct
      241 ggatcggttc ggctttgatt gacgggtccg gctcatagcc cggaatcgca atgcgcggga
      301 tctctttttt cagcagtttt tcgatatcac gcagcagttt gtgttcatca acacacacca
      361 gcgacaacgc ttcaccggta gcagccgcac gaccggtacg cccgatacgg tggacataat
      421 cttccggtac gtttggcagt tcatagttga cgacgtgcgg caactcttcg atatccagac
      481 cgcgcgcagc gatatcggtc gccaccagta cgcgaatgtc acccgattta aaatcagcca
      541 gcgcacgggt acgcgcacct tgcgatttat tcccgtggat cgccgcacta cggatgccat
      601 ctttattgag ctgctcagcc agatggttag cgccgtgttt ggtacgggta aacaccagca
      661 cctgctgcca gttcccttta ccaatcatgt gcgacagcaa ttcgcgtttg cgtttcttat
      721 cgacaaagtg aacgtgctgg gtcacctgat cagacgcggt attgcggcgt gccacttcga
      781 tttccagcgg gttgtgcaac agtttttccg ccagggcttt aatatcgtca gagaaggtcg
      841 cagagaacag caggttctgg cgtttcgctg gcagttttgt taacacgcga cggatatcgt
      901 ggataaagcc catgtcgagc atgcggtcag cttcatcgag gacgaggatt tcaacctgat
      961 ccagcttcac tgcattttga tgttccaggt ccagcaaacg tcccggtgtt gccaccagaa
     1021 catcaacgcc gccgcgcagc ttcatcattt gcgggttaat gctgacaccg ccaaacacca
     1081 ccagcgaacg aatgttcaaa tatttgctgt aatcacggac gttttcgcca atctgcgcag
     1141 ccagttcacg ggtcggggta agaatgagcg cacgtaccgg acgacgccct ttggcgtgcg
     1201 gctggcgagt gatcaggtgt tgcaacagcg gcagcgtaaa gcccgctgtt ttaccggtac
     1261 cggtctgggc gctagccatc aggtcgcggc cttccagcac cgcagggatc gcctgctgct
     1321 gaataggggt gggttcacgg taaccctgct cggcaacggc gcgcaggata tcagggctta
     1381 aacccaaaga atcgaaagac ataactactc cgaaccgccc cgaccgttac cggtgtagtt
     1441 ttcagggaga tactgaaaag gaaaatgaca aaaaccacaa tgtcatgagg gagcagagtg
     1501 tagcagtttt tgtgacgcag cgcataaatt atccctgtga ctggcgtagc taaaattctt
     1561 agccatactg gacaactccc ctatggagtc ataatcttaa tcaatcattt gattaagaat
     1621 ggactgtgcg atgaataatc ctgccatgac aatcaagggt gaacaggcga aaaaacagct
     1681 gattgctgcc gcactggcgc agtttggtga atatggaatg aacgccacca cccgcgagat
     1741 agccgcccag gccgggcaga atatcgctgc catcacctac tacttcggtt cgaaagaaga
     1801 tttgtacctc gcctgcgccc agtggattgc cgattttatt ggcgagcagt tccgtccgca
     1861 cgccgaagag gccgaacgct tgttcgcaca accacagcct gatcgcgcag ccatccgtga
     1921 actgatcctt cgcgcctgca ggaacatgat taagctgctc acccaggatg acaccgtcaa
     1981 cctcagcaag tttatctccc gtgagcagct ctctcccacg gcagcctatc acctggtgca
     2041 cgaacaggtg attagcccgc tacacagcca cctgacacgc ctgattgccg cctggaccgg
     2101 ctgcgacgcc aacgataccc gcatgatcct ccatactcat gcgttgattg gtgagattct
     2161 ggcgttccgt cttggcaaag aaacaattct gttacgtacc ggctggaccg cgttcgatga
     2221 agaaaagacc gaactgatca accagacggt gacctgtcat atcgacctga ttttgcaagg
     2281 attatcgcaa aggagtttgt agtgatgaaa aaacctgtcg tgatcggatt ggcggtagtg
     2341 gtacttgccg ccgtggttgc cggaggctac tggtggtatc aaagccgcca ggataacggc
     2401 ctgacgctgt atggcaacgt ggatattcgt acggtaaatc ttagtttccg tgttgggggg
     2461 cgcgttgaat cgctggcggt ggacgaaggt gatgctatca aagcgggcca ggtgctgggc
     2521 gaactggatc acaagccgta tgagattgcc ctgatgcagg cgaaagcggg cgtctcggtg
     2581 gcacaggcgc agtatgatct gatgcttgcc gggtatcgcg atgaagaaat cgctcaggcc
     2641 gccgcagcgg tgaaacaggc tcaagccgcc tatgactatg cgcagaactt ctataaccgc
     2701 cagcaaggac tgtggaaaag ccgcaccatt tcggcaaatg acctggaaaa tgcccgctcc
     2761 tcgcgcgacc aggcacaggc aacgctgaaa tcagcacagg ataaattgcg tcagtaccgt
     2821 tccggtaacc gtgaacagga catcgctcag gcgaaagcca gcctcgaaca ggcgcaggcg
     2881 caactggcgc aggcggagct gaatttacag gactcaacgt tgatagcacc gtctgatggc
     2941 acgctgttaa cgcgcgcggt ggagccaggc acggttctca atgaaggtgg cacggtgttt
     3001 accgtttcac taacgcgtcc ggtgtgggta cgtgcctatg ttgatgaacg gaatcttgac
     3061 caggcccagc cggggcgcaa agtgctgctt tataccgatg gtcgcccgga caagccctat
     3121 cacggacaga ttggtttcgt ttcgccgact gctgaattta ccccgaaaac cgtcgaaaca
     3181 ccggatctgc gtaccgacct cgtctatcgc ctgcgtattg tggtgaccga cgccgatgat
     3241 gcgttacgcc agggaatgcc ggtgacggtg caattcggta acgaggcagg acatgaatga
     3301 tgccgttatc acgctgaacg gcctggaaaa acgctttccg ggcatggaca agcccgccgt
     3361 cgcgccgctc gattgtacca ttcacgccgg ttatgtgacg gggctggtgg ggccggacgg
     3421 tgcaggtaaa accacgctaa tgcggatgct ggcgggatta ctgaaacccg acagcggcaa
     3481 tgccacggtg attggctttg atccaatcaa aaacgacggc gcgctgcacg ccgtgctcgg
     3541 ctatatgccg cagaaatttg gtctgtatga agatctcacg gtgatggaga acctcaatct
     3601 gtacgcggat ttgcgcagcg tcaccggcga ggcacgtaag caaacttttg ctcgcctgct
     3661 ggagtttacg tctcttgggc cgtttaccgg acgcctggcg ggcaagctct ccggtgggat
     3721 gaaacaaaaa ctcggtctgg cctgtaccct ggtgggcgaa ccgaaagtgt tgctgctcga
     3781 tgaacccggc gtcggcgttg accctatctc acggcgcgaa ctgtggcaga tggtgcatga
     3841 gctggcgggc gaagggatgt taatcctctg gagtacctcg tatctcgacg aagccgagca
     3901 gtgccgtgac gtgttactga tgaacgaagg cgagttgctg tatcagggag aaccaaaagc
     3961 cctgacacaa accatggccg gacgcagctt tctgatgacc agtccacacg agggcaaccg
     4021 caaactgttg caacgcgcct tgaaactgcc gcaggtcagc gacggcatga ttcaggggaa
     4081 atcggtacgt ctgatcctca aaaaagaggc cacaccagac gatattcgcc atgccgaagg
     4141 gatgccggaa atcaacatca acgaaactac gccgcgtttt gaagatgcgt ttattgattt
     4201 gctgggcggt gccggaacct cggaatcgcc gctgggtgca atattgcata cggtagaagg
     4261 cacacccggc gagacggtga tcgaagcgaa agaactaacc aagaaatttg gtgattttgc
     4321 cgccaccgat cacgtcaact ttgccgttaa acgaggggag atttttggtc tgctcgggcc
     4381 aaacggcgcg gggaaatcga ccacctttaa gatgatgtgc ggcttgctgg tgccgacctc
     4441 cggtcaggcg ctggtgctgg ggatggatct gaaagagagt tccggtaaag cgcgccagca
     4501 tctcggctat atggcgcaaa agttttcgct ctatggcaac ctgacggtcg aacagaattt
     4561 acgctttttc tctggtgtgt atggcttacg cggtcgggcg cagaacgaaa aaatctcccg
     4621 tatgagcgag gcgttcggcc tgaaaagtat tgcctcacac gccaccgatg aactgccatt
     4681 aggttttaaa cagcggctgg cgctggcctg ttcgctgatg catgaaccgg atattctgtt
     4741 tctcgacgaa ccgacttccg gcgttgaccc cctcacccgc cgtgaatttt ggctgcacat
     4801 caacagcatg gtagagaaag gcgtcacggt gatggtcacc acccacttta tggatgaagc
     4861 ggaatattgc gaccgcatcg gcctggtgta ccgcgggaaa ttaatcgcca gcggcacgcc
     4921 ggacgatttg aaagcacagt cggctaacga tgagcaaccc gatcccacca tggagcaagc
     4981 ctttattcag ttgatccacg actgggataa ggagcatagc aatgagtaac ccgatccttt
     5041 cctggcgtcg cgtgcgggcg ctgtgcgtca aagagacgcg gcagatcgtt cgcgatccga
     5101 gtagctggct gattgcggta gtgatcccgc tgttgctgct gtttattttt ggttacggca
     5161 ttaacctcga ctccagcaag ctgcgggtcg ggattttact ggaacagcgt agcgaagcgg
     5221 cgctggattt cacccacacc atgaccggtt cgccctacat cgacgccacc atcagcgata
     5281 accgtcagga actgatcgcc aaaatgcagg cggggaaaat tcgcggtctg gtggttattc
     5341 cggtggattt tgcggaacag atggagcgcg ccaacgccac cgcaccgatt caggtgatca
     5401 ccgacggcag tgagccgaat accgctaact ttgtacaggg gtatgtcgaa gggatctggc
     5461 agatctggca aatgcagcga gcggaggaca acgggcagac ttttgaaccg cttattgatg
     5521 tacaaacccg ctactggttt aacccggcgg cgattagcca gcacttcatt atccccggtg
     5581 cggtgaccat tatcatgacg gtcatcggcg cgattctcac ctcgctggtg gtggcgcgag
     5641 aatgggaacg cggcaccatg gaggctctgc tctctacgga gattacccgc acggaactgc
     5701 tgctgtgtaa gctgatccct tattactttc tcgggatgct ggcgatgttg ctgtgtatgc
     5761 tggtgtcagt gtttattctc ggcgtgccgt atcgcgggtc gctgctgatt ctgtttttta
     5821 tctccagcct gtttttactc agtaccctgg ggatggggct gctgatttcc acgattaccc
     5881 gcaaccagtt caatgccgct caggtcgccc tgaacgccgc ttttctgccg tcgattatgc
     5941 tttccggctt tatttttcag atcgacagta tgcccgcggt gatccgcgcg gtgacgtaca
     6001 ttattcccgc tcgttatttc gtcagcaccc tgcaaagcct gttcctcgcc gggaatattc
     6061 cagtggtgct ggtggtaaac gtgctgtttt tgatcgcttc ggcggtgatg tttatcggcc
     6121 tgacgtggct gaaaaccaaa cgtcggctgg attagggaga agagcatgtt tcatcgctta
     6181 tggacgttaa tccgcaaaga gttgcagtcg ctgctgcgcg aaccgcaaac ccgcgcgatt
     6241 ctgattttac ctgtgcttat tcaggtgatt ctgttcccgt tcgccgccac gctggaagtg
     6301 actaacgcca ccatcgccat ctacgatgaa gataacggcg agcattcggt ggagctgacc
     6361 caacgttttg cccgcgccag cgcctttact catgtgctgc tgctgaaaag cccacaggag
     6421 atccgcccaa ccatcgacac acaaaaggcg ttactactgg tgcgtttccc ggctgacttc
     6481 tcgcgcaaac tggatacctt ccagaccgcg ccgttgcagt tgatcctcga cgggcgtaac
     6541 tccaacagtg cgcaaattgc cgccaactac ctgcaacaga tcgtcaaaaa ttatcagcag
     6601 gagctgctgg aaggaaaacc gaaacctaac aacagcgagc tggtggtacg caactggtat
     6661 aacccgaatc ttgactacaa atggtttgtg gtgccgtcgc tgatcgccat gatcaccact
     6721 atcggcgtaa tgatcgtcac ttcactttcc gtcgcccgcg aacgtgaaca aggtacgctc
     6781 gatcagctac tggtttcgcc gctcaccacc tggcagatct tcatcggcaa agccgtaccg
     6841 gcgttaattg tcgccacctt ccaggccacc attgtgctgg cgattggtat ctgggcgtat
     6901 caaatcccct tcgccggatc gctggcgctg ttctacttta cgatggtgat ttacggttta
     6961 tcgctggtgg gattcggtct gttgatttca tcactctgct cgacacaaca gcaggcgttt
     7021 atcggcgtgt ttgtttttat gatgcccgcc attctccttt ccggttacgt ttctccggtg
     7081 gaaaacatgc cagtatggct gcaaaacctg acgtggatta accctattcg ccactttacg
     7141 gacattacca agcagattta tttgaaggat gcgagtctgg atattgtgtg gaatagtttg
     7201 tggccgctac tggtgataac ggccacgaca gggtcagcgg cgtacgcgat gtttagacgt
     7261 aaggtgatgt aacttcttat ctttcgccag caaagacact accgccgggc cggcaaggat
     7321 tgccagccct gcaattgcca gcaaaaagtt gttttccagt acacgggaca acagccacag
     7381 cacaatcagc gctgcgccaa aataccaggt cgtggtcagc tcttccagca cgtctgccac
     7441 ctcacgccag cgttgctcgg ggtggcgctg aaacaccaac atcatcacta ccgtgacaca
     7501 atacagtgcg cataatccca cgccgacgtg aaccagagtc ttactcatcc agcccaccac
     7561 cagcagcgcc actaccagta aatgcaacat aatctgccag caacttagac cagttgcgac
     7621 acgaacacgt tgttgccact tcatggcttc tctcctgaag aatttatctc tacttactca
     7681 gagtaccgaa ctttacggaa aattccgtaa gaccgcacct ttttgtgacg ccgactacac
     7741 tatttcaatg agataagagg caaagacagg aaaattatgc ccgatcaaac acaacaattt
     7801 tcgttcaagg tgctcaccat caacattcac aaaggtttta ccgcgtttaa ccgacgcttc
     7861 attttgccgg aacttcgcga cgcggtgcgt accgtcagcg ccgatattgt ttgcctgcag
     7921 gaagtgatgg gcgcgcacga agttcatccg ctgcatgtgg aaaactggcc cgatacctcg
     7981 cactacgagt ttctcgccga cacgatgtgg agcgattttg cctacggtcg caatgccgta
     8041 tacccggaag ggcatcacgg caacgccgta ctgtcgcgtt atcccattga acattatgag
     8101 aatcgcgatg tttcggtcga tggtgcggaa aagcgcggcg tgctctactg ccgcattgtg
     8161 ccaccgatga ccggaaaagc gattcatgtg atgtgcgtac atctgggcct gcgtgaggcg
     8221 caccgtcagg cgcagcttgc gatgctcgcc gaatgggtga atgagctacc ggacggcgaa
     8281 ccggtattgg tggcgggtga tttcaatgac tggcggcaaa aagctaatca tccgttaaaa
     8341 gtgcaggccg gactggatga gatttttacc cgcgcccacg gacgcccggc gcgcacgttt
     8401 ccggtgcaat ttcctctact acgactggac aggatctacg tcaaaaatgc cagcgccagc
     8461 gcgccaaccg cgttgccgct gcggacatgg cgacaccttt ctgatcatgc ccctttaagt
     8521 gcggagattc atttatgaaa tgtagctggc gcgaaggcaa taagatccag ttgctggaaa
     8581 acggcgagca atattatccc gcggtgttta aggcgattgg cgaggcacaa gaacgcatca
     8641 ttcttgaaac gtttatctgg tttgaggatg acgtcggcaa acagctgcat gcggcactac
     8701 tggcagcagc gcaacgcggg gttaaagcgg aagtcttgct ggatggctac ggttcgccgg
     8761 atctcagcga tgagtttgtc aatgaactga cggcagctgg cgtggtattt cgctactacg
     8821 atccccgccc tcgccttttt ggtatgcgca ccaatgtgtt tcgccggatg catcgcaaaa
     8881 ttgtggtgat cgacgcgcgt atcgccttta ttggcgggct gaattactcc gccgagcata
     8941 tgtccagcta cggtccagag gctaaacagg attacgcggt acgccttgaa gggccgattg
     9001 tcgaagatat tctccagttt gagctggaaa acctgcctgg acagagcgcg gcacgacgct
     9061 ggtggcgacg tcatcacaaa gcggaagaga accgccagcc gggagaagcg caggtattgc
     9121 tggtctggcg cgataacgaa gaacatcgcg atgatattga acgtcactat ctgaaaatgc
     9181 tcactcaggc gcggcgagaa gtcattatcg ccaacgccta cttcttcccc ggctatcgat
     9241 ttttacacgc cttgcgtaaa gcggcgcggc gcggggtgcg gatcaaactg atcattcagg
     9301 gcgaaccgga tatgccgatt gtcagagtcg gtgcgcgctt gctgtataac tatctggtta
     9361 aaggcggcgt tcaggtattt gagtaccgcc gccgcccgct ccacggcaaa gtggcattga
     9421 tggacgatca ctgggcgaca gtagggtcca gtaatctcga tccgctcagt ttgtcactga
     9481 atctcgaagc aaatgtcatc atccatgatc gtcattttaa ccagacgctg cgcgataatc
     9541 tgaacggcat tattgccgca gattgtcagc aggtcgatga gaccatgctg ccgaaacgca
     9601 cctggtggaa cctgaccaaa agcgtgctgg cgttccactt tttacgccac ttcccggcgc
     9661 tggttggctg gcttccggca cacacaccac gtctggcgca ggttgatccg cccgcacaac
     9721 cgacaatgga aacgcaggat cgggtagaaa ctgaaaacac gggggtaaaa ccctgatgag
     9781 taaatcacac ccgcgctggc gcttagcaaa gaagatcctc acctggctgt tttttatcgc
     9841 ggtgattgtg ttgctggtgg tctacgccaa aaaagtggac tgggaagagg tctggaaggt
     9901 catccgcgac tacaatcgcg ttgcgctgct tagtgcggtc gggctggtgg tcgtcagcta
     9961 tctgatttac ggctgctatg acctgctcgc ccgcttttac tgcggtcaca aactggcgaa
    10021 gcgccaggtg atgctggtgt cgtttatctg ctacgccttc aacctgacgc tcagtacctg
    10081 ggtcggcggc attggtatgc gctatcgttt gtactcccga ctagggttac cgggcagcac
    10141 tattacgcgg attttctcgc tcagtattac caccaactgg ctgggctata ttttactggc
    10201 agggattatc tttaccgcag gcgtggtgga gttgccggat cactggtatg tcgatcaaac
    10261 tacgctgcgt attctcggca ttggcttact gatgattatc gcggtttatt tgtggttttg
    10321 cgctttcgcg aagcaccgcc atatgaccat caaagggcaa aagctggtgc tgccttcatg
    10381 gaaattcgcc ctcgctcaga tgctgatttc cagcgttaac tggatggtaa tgggggcgat
    10441 tatctggctg ttacttggtc aaagcgtgaa ctatttcttt gtactgggcg tgttactggt
    10501 tagtagtatt gctggcgtca tcgtgcatat tccggcgggg atcggtgtgc tggaagcggt
    10561 gtttatcgcg ctactggctg gggagcatac atccaaaggc acaattatcg ccgccctact
    10621 cgcttatcgc gtcctgtatt actttatccc gctgctgctg gcgctgatct gttacctgct
    10681 actggaaagc caggcgaaga agctacgggc gaaaaatgaa gcggcgatgt gaagtttagt
    10741 taagttcttt agtatctgca tttacgacta atgaaaaaaa cgcgtatgcc tttgccagac
    10801 aagcgttata gctatcgcgg caagtatccc aaagaagccg ataaaatagt aatagagtgt
    10861 taatgcacct gtaacagcgc aacgaaccgg actccacaac tcttcactat ggcatttccc
    10921 cgccaattca aggagcttat ttcgtccatg cgagataatg cctgaccata acacaatcat
    10981 taagcaactt attacccaaa cgggtcgttc actcattaat gcggcattca tgatgatcac
    11041 cagggccaaa cctgtcaacg tcatcataat gataaaacgg tgagaaccag gatcaacatt
    11101 aaataataaa ccggccagca tactgattaa aaacatcgcg cccgcagtgc tgaacacgac
    11161 gataatggca ggtacaggag ctaaacttgc aacgacaatc gccaccaaag agagtaaaaa
    11221 cagatatatt acgcctgcga tgacggcatt tatcttgttg atatccttac ttaagccata
    11281 catacaaact aataataacc ctatggcgac atacattgtt ggtgaatcat atttggttgc
    11341 cgtaatttcg agatacttcg ctacccaggc cagtcctgcg gttgtcacca aacctaaagc
    11401 ggtaatgagc catatgctgt ttaatatcct ggcttcgtgc tgaaaatcat tagttatgtt
    11461 atttgctgag cgcgaattca ttatttttat atccttaagt taaatatcat tatctattcc
    11521 aattaacgca
//
LOCUS       DAZFRN010000150        11475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFRN010000150 DAZFRN010000000
VERSION     DAZFRN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.152"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            242..1108
                     /gene="folD"
                     /locus_tag="ACHKUB_004007"
     CDS             242..1108
                     /gene="folD"
                     /locus_tag="ACHKUB_004007"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO6656963.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1110..1322
                     /gene="ybcJ"
                     /locus_tag="ACHKUB_004008"
     CDS             1110..1322
                     /gene="ybcJ"
                     /locus_tag="ACHKUB_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO6656964.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1430..1951
                     /locus_tag="ACHKUB_004009"
     CDS             1430..1951
                     /locus_tag="ACHKUB_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6656965.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1987..3372)
                     /gene="cysS"
                     /locus_tag="ACHKUB_004010"
     CDS             complement(1987..3372)
                     /gene="cysS"
                     /locus_tag="ACHKUB_004010"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO6656966.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3546..4040
                     /gene="ppiB"
                     /locus_tag="ACHKUB_004011"
     CDS             3546..4040
                     /gene="ppiB"
                     /locus_tag="ACHKUB_004011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO6656967.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4043..4765
                     /gene="lpxH"
                     /locus_tag="ACHKUB_004012"
     CDS             4043..4765
                     /gene="lpxH"
                     /locus_tag="ACHKUB_004012"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO6656968.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4883..5392
                     /gene="purE"
                     /locus_tag="ACHKUB_004013"
     CDS             4883..5392
                     /gene="purE"
                     /locus_tag="ACHKUB_004013"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO6656969.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5389..6456
                     /gene="purK"
                     /locus_tag="ACHKUB_004014"
     CDS             5389..6456
                     /gene="purK"
                     /locus_tag="ACHKUB_004014"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO6656970.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQN
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(6661..7554)
                     /locus_tag="ACHKUB_004015"
     CDS             complement(6661..7554)
                     /locus_tag="ACHKUB_004015"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6656971.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7551..8366)
                     /locus_tag="ACHKUB_004016"
     CDS             complement(7551..8366)
                     /locus_tag="ACHKUB_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6656972.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8377..9636)
                     /locus_tag="ACHKUB_004017"
     CDS             complement(8377..9636)
                     /locus_tag="ACHKUB_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6656973.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNQGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMEHGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9646..11313)
                     /locus_tag="ACHKUB_004018"
     CDS             complement(9646..11313)
                     /locus_tag="ACHKUB_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6656974.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTLAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2561 a         3275 c         3030 g         2609 t
ORIGIN      
        1 caggtatttc ttaaattatc ttaatcctta aacaaggaaa taaatcagtt ccagatttac
       61 aacgccatca tggacgaaaa atgaagcttt caatctcagc gacctcagcg acggtgcgcc
      121 taaccttcgc aagaggtcgc ttcacgcgat aaatctgaaa cgaaacctga cagcgcgccc
      181 cgcttctgac aaaataggcg catccccttc gacctacgta acagatggaa tcctctctct
      241 gatggcagca aagattattg acggtaaaac gattgcgcag caggtgcgct ctgaagttgc
      301 tcaaaaagtt caggcgcgta ttgcagccgg actgcgggca ccaggactgg ccgttgtgct
      361 ggtgggtagt aaccctgcat cgcaaattta tgtcgcaagc aaacgcaagg cttgtgaaga
      421 agtcgggttc gtctcccgct cttatgacct cccggaaacc accagcgaag cggagctgct
      481 ggagcttatc gatacgctga atgccgacaa caccatcgat ggcattctgg ttcaactgcc
      541 gttaccggcg ggtattgata acgtcaaagt gctggaacgt attcatccgg acaaagacgt
      601 ggacggtttc catccttaca acgtcggtcg tctgtgccag cgcgcgccgc gtctgcgtcc
      661 ctgcaccccg cgcggtatcg tcacgctgct tgagcgttac aacattgata ccttcggcct
      721 caacgccgtg gtgattggcg catcgaatat cgtcggtcgc ccaatgagca tggaactgtt
      781 gctcgcaggt tgcaccacta ccgtgactca ccgcttcact aaaaatctgc gtcatcacgt
      841 agaaaatgcc gatctgttga tcgttgccgt tggcaagcca ggctttattc ccggtgactg
      901 gatcaaagaa ggcgcaattg tgattgatgt cggcatcaac cgtctggaaa atggcaaagt
      961 tgtgggcgac gtcgtgtttg aagacgcggc taaacgcgcc tcatacatta cgcctgttcc
     1021 cggcggcgtt ggcccgatga cggttgccac gctgattgaa aacacgctac aggcgtgcgt
     1081 tgaatatcat gatccacagg atgagtaaca tggcgacatt ttctttaggt aaacatccgc
     1141 acgttgagct gtgcgacttg ctgaaactgg aaggctggag cgaaagcggc gcgcaggcga
     1201 aaatcgcgat tgccgaaggc caggtgaaag tcgacggtgc ggttgaaacg cgcaaacgct
     1261 gcaaaatcgt cgccggtcag acagtgagtt ttgcaggtca cagcgtacag gttgttgcct
     1321 gatccctttc ccggcctgta tcaacaggcc ggttctcttt tcagactttt gccctctacg
     1381 ccccgttcat ccatgctgat agcatcgaca ttttgccgaa gcgaccatca tgccaaccgt
     1441 tattacgcac gccgccgttc ccctatgcat tggtttaggg ttgggttcga aagtcatccc
     1501 tccgcgtctg ttatttgccg gaatcatcct ggcgatgctg cccgacgccg acgtattgtc
     1561 gtttaaattt ggcgttgctt acggcaatgt ttttggtcat cgcgggttta cccattcgct
     1621 ggtgtttgcg tttgttgtcc cgctgttatg tgtgctgatt ggacgccgat ggttcagggc
     1681 tgggctgatt cgctgctggc tgtttttaac cgtctcgttg ctatcgcaca gcttgctgga
     1741 ttcggtaacc actggcggta agggcgttgg ctggctgtgg ccgtggtcag atgaacgctt
     1801 tttcgctccc tggcaggtga ttaaagtcgc gccgtttgca ttgtctcgtt acaccacgcc
     1861 gtacgggcat caggtgataa tttctgaatt gatgtgggtg tggttgccgg ggatggtgct
     1921 tatgggaatg ttgtggtggc gcaggcgata accggatgcg aaaactcgca tccggcaaca
     1981 gcgcaattac ttacgacgcc aggtggtccc ttgcgggcca tcttccagca cgatccccat
     2041 ctcgttaaga cggtcacgcg ccgcatctgc cgccgcccag tctttcgctt tacgggcatc
     2101 cagacgctgt tgaattaacg cttcaatctc agccacttcg ctgtcgtctg cctgcgcgcc
     2161 gctttgcagg aacgcttccg gttcttgctc cagcaggccc aatacagcgg aaagtttacg
     2221 caggtgagat gccattgcat tcgccgctgc catatcttct gctttcagac ggtttacttc
     2281 acgcgccata tcaaacagta cggaataggc ttccggggtg ttgaaatcgt cgtccatcgc
     2341 ttcaataaag cgcgcttcaa acgcttcgcc accggcaggc gcaacggttt tatctgtgcc
     2401 gcgcagcgca gtgtagagac gctccagcgc cgcacgcgcc tgcttcaggt tctcttcgct
     2461 gtagttcaac tggctgcgat agtggcccga catcaggaag taacgcacgg tttccgcgtc
     2521 gtagtatttc agcacatcgc gcacggtaaa gaagttaccc agcgatttgg acatcttctc
     2581 gcggtcaacc atcaccatcc ccgagtgcat ccagtagttc acatactggc catcatgggc
     2641 acaggtggac tgcgcgattt cgttctcgtg gtgcgggaac atcaggtctg aaccgccgcc
     2701 gtggatatca aagtggttac ccagctgctt gcagttcatt gccgaacatt caatgtgcca
     2761 gcctggacgg cccgcgcccc acggagacgg ccagctcggt tcgccctctt tcgacatctt
     2821 ccacagaacg aagtccatcg ggttgcgttt gtcatcaacc acgtcaacgc gcgcgccagc
     2881 ctgcagctgg tcgagatcct gacgcgacag cacgccataa gttggatcgg tcgggacgtc
     2941 gaacatcacg tcgccgttgt ccgccacata agcgtgacct ttggcgatca gttgttcagt
     3001 gagttcaata atttctgcga tatggtgcgt cgcgcgcggc tccatatccg ggcgcagaat
     3061 gttcaaagca tcaaaatctt tgtgcatttc ggcgatcatg cgatccacca gcgccacaaa
     3121 gctttcgcca ttttcattgg cgcgtttgat gattttgtcg tcgatatcgg taatgttgcg
     3181 cacatacttc agtttatagc cgaggaaacg cagatagcgc gcaaccacgt caaaagcaac
     3241 aaaggtacgc ccgtgaccga tatgacagag atcgtaaacg gtgattccac acacgtacat
     3301 gccgacttcc ccggcgtgaa taggcttaaa ttcctctttt tggcgtgtca gagtattgaa
     3361 gatttttagc atcgaagatt ccgtttagac atgtgtgggt aattgagttg cgtataatac
     3421 ccatatttcc cgcgcgaatc agcatacatt gcgtgatgat ccgatcgtgc ggttatgcta
     3481 taacaccacc ctatatatga cccgaactgg gttgaagcac caatcaaacg gaacaggatg
     3541 caaaaatggt tactttccac accaatcacg gcgatattgt catcaaaact tttgacgata
     3601 aagcacctga aacagttaaa aacttcctgg actactgccg cgaaggtttt tacaacaaca
     3661 ccattttcca ccgtgttatc aacggcttta tgattcaggg cggcggtttt gaaccgggca
     3721 tgaaacaaaa agccaccaaa gaaccgatca aaaacgaagc caacaacggc ctgaaaaata
     3781 cccgtggtac gctggcaatg gcacgtactc aggctccgca ctctgcaact gcacagttct
     3841 tcatcaacgt ggttgataac gacttcctga acttctctgg cgaaagcctg caaggttggg
     3901 gctactgcgt gtttgctgaa gtggttgacg gcatggacgt ggtagacaaa atcaaaggtg
     3961 ttgcaaccgg tcgtagcggt atgcaccagg acgtgccaaa agaagacgtt atcattgaaa
     4021 gcgtgaccgt tagcgagtaa tcgtggcgac actctttatt gcagatcttc atctctgcgt
     4081 ggaagaaccg gcgatcaccg ccggttttct gcgtttttta gcgggggaag cccgcaaggc
     4141 cgacgcgctg tatattcttg gcgatctgtt tgaagcatgg attggcgacg acgatcccaa
     4201 cccactccat cgccagatgg cggcagcgat caaagcggtg tccgattccg gcgttccctg
     4261 ttatttcatt catggcaacc gtgattttct gctcggcaaa cgctttgccc gtgaaagcgg
     4321 catgacatta ttgccggaag aaaaggtgct cgaactttat ggtcgccggg tgttgattat
     4381 gcatggcgac acgctgtgca ccgatgacgc gggttatcag gcttttcgcg ccaaagtcca
     4441 caaaccctgg ctgcaaacgc tattcctcgc cctgccgttg tttgtgcgca aacgcattgc
     4501 cgcgcgaatg cgagcgaaca gtaaagaagc caacagcagt aaatcgctgg cgatcatgga
     4561 cgttaaccaa aacgcggtag tcagtgcgat ggaaaaacat caggtgcaat ggctgatcca
     4621 cgggcatacc catcgcccgg cggtacatga acttatcgcc aaccagcaac ctgcttttcg
     4681 cgtggtactc ggtgcctggc atacagaagg ttcgatggtg aaagtcacgg cggatgacgt
     4741 tgagctgatt cattttccgt tttaaaaaac ccgcaacttt gctgatttca cagccacgca
     4801 accgttttcc ttgctctctt tccgtgctat tctctgtgcc ctctaaagcc gagagttgtg
     4861 caccacagga gttttaagac gcatgtcttc ccgcaataat ccggcgcgtg tcgccatcgt
     4921 gatggggtcc aaaagcgact gggctaccat gcagttcgcc gccgaaatct tcgaaatcct
     4981 gaatgtcccg caccacgttg aagtggtttc tgctcaccgc acccccgata aactgttcag
     5041 cttcgccgaa agcgccgaag agaacggtta tcaggtgatt attgcgggcg caggcggcgc
     5101 agcgcacctg ccaggcatga ttgccgccaa aacgctggtg ccggtgctgg gcgtgccagt
     5161 acagagcgcc gcactgagcg gtgtcgatag cctctactcc atcgtacaaa tgccgcgcgg
     5221 cattccggtg ggtacgctgg cgattggtaa agctggcgcg gcaaacgcgg cattactggc
     5281 agcacaaatt cttgcgactc atgataaaga actgcaccag cgtctgaatg actggcgcaa
     5341 agcccagacc gacgaagtgc tggaaaaccc ggacccgcga ggtgcggcat gaaacaggtt
     5401 tgcgtcctcg gtaacgggca gttaggccgt atgctgcgtc aggcaggtga accgttaggc
     5461 attgctgtct ggccggtcgg gctggacgct gaaccggcgg cggtgccttt tcaacaaaac
     5521 gtgattaccg ctgagatcga acgctggccg gaaaccgcat taacccgcga gctggcgcgt
     5581 catccggcct ttgtgaaccg cgatgtgttc ccgattattg ccgaccgtct gactcagaag
     5641 cagcttttcg ataagctcca cctgccgacc gcaccgtggc agttacttgc cgatcgcagc
     5701 gagtggcctg cggtgtttga gcgtttaggt gaactggcga ttgttaagcg tcgcactggt
     5761 ggctatgacg gtcgcggtca atggcgttta cgtgccaatg aaaccgaaca gttaccggca
     5821 gagtgttacg gcgaatgtat tgtcgagcag ggcattaact tctctggtga agtgtcgctg
     5881 gttggcgcac gcggatttga tggcagcacc gtgttttatc cgctgacgca taacctgcat
     5941 caggacggta ttttgcgcac cagcgtcgct tttccgcagg ccaatgcgca gcagcaagcg
     6001 caagccgaag agatgctgtc ggcgattatg caggagctgg gctatgtggg cgtgatggcg
     6061 atggagtgtt ttgtcacccc gcaaggtctg ctgatcaacg aactggcacc gcgtgtgcat
     6121 aacagcggtc actggacaca aaacggtgcc agcatcagcc agtttgagct gcatctgcgg
     6181 gcgattaccg atctgccgtt accgcaaccg gtagtgaata gtccgtcggt gatgatcaac
     6241 ctgattggta gcgatgtgaa ttatgactgg ctgaaactgc cgctggtgca tctgcactgg
     6301 tacgacaaag aagtccgtcc ggggcgtaaa gtggggcatc tgaatttgac cgacagcgac
     6361 acatcgcgtc tgactgcgac gctggaagcc ttaatcccgc tgctgccgcc ggaatatgcc
     6421 agcggcgtga tttgggcgca gagtaagttc ggttaactgg tgctctattc ttgccggatg
     6481 cggcgtaaac gccttatccg gcctaccgat ccggtaccca ttgtaggcct gataagatgc
     6541 gtcaagcatc gcatcaggca ttgtgcacca attgccggat gcggcaccgg ttgtaggcct
     6601 gataagacgc gtcaagcgtc gcatcaggca tggtgcaccg attgccggat gctctcatca
     6661 ctacagcgaa atacaggtcc ccgcttcgcc cgccagcgtc tcttcaattc gcgataacgc
     6721 cccaatccac gcgggtttac cacggcttct gacataacca cttaccgccg ttaccttcgg
     6781 ccccatcgaa ccatcggctt tggcaaatgg cgctaactca tccggtgtgg catggcgaat
     6841 ggcgcgttgc tgcggcgttc cccagttttc atataccgca tcagcatcgg tgaggatcac
     6901 cagcccatcg gcgttaatct gctcggcaag caaggcagcg gcgagatctt tatcaatcac
     6961 cgcttcactc cctgccccat cttccgtcac aggcacaccg ccgccgccac tgcaaatcac
     7021 cacatgcccc tctttgagca acaactcgat ggcttcgctg tcgagaattt tacgcggttg
     7081 cggagacgcc accacccggc gcaaatattt accatcacgt ttcatctgcc agccgtaagc
     7141 cgcttccagt gcctcttgtt cttctggctg ataaaccgga ccaataaatt tctctggctg
     7201 caaaaacgcc ggatcatcag gcgaaacctc aatgcgcgtc agcaccgtcg tcacgggcgg
     7261 catctgcggc tgtgcgctca aactctgcgc cagcatatag ccaatcatcc cctgactttc
     7321 tgccaccagc acatccagcg gatacggttc tacctctttc cacgccagat tctgcaatgc
     7381 cagcagcccc acctgcggcc cgttgccgtg aacaatcgcc agccgataag aacgggccag
     7441 gcgtgccagc gcgggtacag cactggcgat attgcgatat tgattttccg ccgtcagcgc
     7501 ctcaccgcgc tggagtaagg cgttgccccc aagagctaca accagtgttt tcatggtttt
     7561 ccttgtaata attgttgacc aagccagaac cccagcagtg tatctgcccc tgacgtatgc
     7621 cccagcgcca gcagcgaatc aatcgcaaca gcggtacgtt tcgggcaact cagagcatga
     7681 acaaagtgca ggagtggcga ggcgaaatat ccttgcgcgg cataacgtaa ataactgacg
     7741 ctcaccgctg tggtaacgag ttgcagattg tcggaacagg caaaaaactg gcgaccggag
     7801 cgcgcatcta aagcgccata ataccaggcc gccagcagca ttccgctcag cgtgtcatca
     7861 tgactcggcg ttaatccggg gcctttaccc agccagtgcc gccagtcggt cttaacgcca
     7921 ttgagcgcgg cctgaaaaca gtgacgaaac tggcgtaact cagcaggcag cggatcgctt
     7981 gccgccaacg ccagtggccc gaaaagcccg gtttcctccg cgcgttgcat ccatgcagct
     8041 gcaagcggtt gaggatgcgc aggcggcgta atacgcagca aacaataacg ctgtggctgt
     8101 ttaaccgtga aacgcccgag gcgaatccct tgagccacaa cctgtggtcg ttcattgccg
     8161 cataatccac cgcataacgc atcgaattgc gcgcggcgaa gcacccatcc tccggggccg
     8221 aaaccactac cctgacgatg cagcgttaac agttcgccgc tttccgtcat caggttaatc
     8281 gcccgtcgcc acactcccgc taaccgccag gactggcgat aattcggtgc actacggctg
     8341 gcaagcagag gatggatgat cgtcattgcg cgttcctcaa ccaatcccca tgctttcagc
     8401 cagtgcttcc agcgcctgtt caaaacacgc cagcggtgcc cgcacagtgc ctgcgccaat
     8461 ctgcccgatc cccgcctctt tatgggcgat accggtattg atgagtggcg taatgccggt
     8521 ttctaccacg cgacgaatgt ccagtcccag gcacgcgccc tgaaaatccc agcctgggat
     8581 ctgcaactgc atattgcgct caaggtaaat ttccgccatc tcttcagata ccgctctggc
     8641 cgcttccatg ccacccgcac cgacaaagcg cgttacgcca ggcgctgcga tcattgccgc
     8701 acctccgata ccaaaggttt cggtaatcgc gctatcgccc atatccgggt tcgcctgctc
     8761 ctgcgagaag ccggtgaaaa acagaccttg cggagtgttt acaggcgcag taaaccagcg
     8821 ttcgcccagc ccgcttaccc gaatcccgaa catattgccg ttgcgggtca ttgccgtgac
     8881 gatgctgcct gcgcggatca tcgcgccagc atccatcgcc gccttgcagt aagccatcgc
     8941 gaggttgagg aagaactgat cggtcacgct gaggaaatcc atcacttcgg cgatgtgctg
     9001 tttatcatga tcgaggcgag caatttgtgg ggccagcgca cgcattaaca gtgctgagga
     9061 agcaatattg cgttgatgga actcatcgcc catggtaatg ccctgcgcca tcatcgccgt
     9121 gagatcgata ccgtgctcca tgcgccccag cgccgcgctt aatactggca ttaacacatc
     9181 gcgcatccag cgatggcgag tcaggacatc ttcgccgtaa gcgccaaaac gcatcacttt
     9241 gccgatacct tcgttgaggt tgcagtacgc ccggttgccg tcggtcacgt tctcgaccac
     9301 cagcatcggc atactggcag aagtaatgcc gcccattggc ccgacggcat tcacatggtg
     9361 acaaggaatg aagttcactt ccccctgctc cagcattgcc agtgcctgcg cttcatcttt
     9421 cgcccagcct tcaaacagac atgcacccac acatgcccct ttcatgggtc cggtcatctc
     9481 ctgccagcgc attggcggcc cggcgtgaag cagtgttttg ccctggttta gttcgctgat
     9541 aagtgaagaa gccggttgca catccagcca gtgtggacga gcgcgacgaa tttgttcgat
     9601 aaccgcagca ttggcttgcg ccactgatgt aaacatggga accccttatt gcaaacgttc
     9661 taataaacga gccagttttt tattgccgcc ggcgactggc gaccattggt aatgcacaac
     9721 cggtttgctg gcgctttgta gctccagcgc aaagctgcgt aatccgatgt taatcacggc
     9781 gacgttttcc agtaatgacg gtgtgtgttg ctgtgtggca gacgagagcg gatgaattaa
     9841 cgctgccgcc agcaaggtgg cttccggtag cgaactcact accgcgatcc ccgcatcttc
     9901 cagcgtggcg atttgctgcg agcggcattg cgggtcacgt tcggtacctg tcaccgtggc
     9961 aatggcgtac agcggttggc tgtctgaacg cgcggcacag gctttttgcc aggcgctcac
    10021 cagcgaggca gcaggatcgg cggtcgcacc gaagccaatc acgacatcaa gcagcaacac
    10081 gcgcacttgc ggtttagcgc cgagatcggc aattaactgg ttgcgtaagg ctgggtcgat
    10141 catcggatgg ggacgcccga cggtgtagaa atcatcgccc agatcaatga tctggtgacc
    10201 atcggcgtcc agcatcatgc cgtgatgatg ggtatcgtcg gcttccacgc caaggtgccc
    10261 ggcaagtaat cccgccgctt cggcagccag cgtaccgccg gtatacaaac cgcaaataaa
    10321 tccgctgctg gcaggcgtta tcgcgttacg tcgcgccgta acgcgtgaaa gcaggcaagc
    10381 caggcgtgcg gcctcatcca gcgaggaggc aaaccagaca ttctcgtcgc gggccaccgc
    10441 cagggtataa cctaaaaaca gcgccaccgt cggtttgccg gttgctttca tggcattaac
    10501 aattttcaga cgcacagctt cggcaggcgg ttttgaaaca aatgccagca cttcgctttt
    10561 ctcgtctgca ctgagcattt ccagcgccgt cagcgcactg atgccgccca cttcacggct
    10621 gaggtcgcgc ccgccaaggc caatcgcgtg agtaattccc tcccctgcca gcgcaatctg
    10681 tgagcacagc tcctgaatcc cggtaccgga agcgccaatg acgccaatgt tgccttccgg
    10741 catcacgtta gcaaaagcca gcggcgtggc ggcaatcatc gacgtaccgc agtccggccc
    10801 catcaccagc aagccttttt ctcgcgcgcg ggttttaagt tggatttcat cttccagcgt
    10861 gacgttatcg gagaacatca tcacgttgag gttgcgatcc agcgcctgat tcgccagctc
    10921 cgccgcatac tcgccagcca ctgaaatcag cgccagattg gcatcggata atttctgaca
    10981 ggcactgtcc cagcgacgca cctgcgtcaa ggactggctg ctgcctgacc cctgcgccag
    11041 ttgttttagc gcctcttcaa gctgctgcat aatcgcctgc gcgatccccg catccgccgc
    11101 ttcgctacga attgccacgc aaatatcgtt cggcgtggcg ttattaaaat cgtcatgcca
    11161 gaaacctgtg gtatctaata acgctttatt ggcgggcgta cccatcatta cggaaacatc
    11221 atcaacattt tctgattcgc tgagttttcg tgaaataatc attaaactga ccgaatcctg
    11281 aaaacaccct tttttaataa aggcgtggat cataacaact ccttaaaaat tgcacccatg
    11341 gcaattgcac gagcagtgtt aatgtggcga ttacggtatc gatgacaacg gataaataag
    11401 gtgatgaata tcacacaggg attattaaca aaagaattca aataaccgcc atgctattac
    11461 gtgttatttt ttaat
//
LOCUS       DAZFRN010000151        11419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFRN010000151 DAZFRN010000000
VERSION     DAZFRN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.153"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..519)
                     /locus_tag="ACHKUB_004019"
     CDS             complement(<1..519)
                     /locus_tag="ACHKUB_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656975.1"
                     /translation="MSYFLRRKWMVNLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FIFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGLKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYD"
     gene            762..1106
                     /locus_tag="ACHKUB_004020"
     CDS             762..1106
                     /locus_tag="ACHKUB_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6656976.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            1229..1429
                     /gene="torI"
                     /locus_tag="ACHKUB_004021"
     CDS             1229..1429
                     /gene="torI"
                     /locus_tag="ACHKUB_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO6656977.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(1959..3206)
                     /locus_tag="ACHKUB_004022"
     CDS             complement(1959..3206)
                     /locus_tag="ACHKUB_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO6656978.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3278..4192)
                     /locus_tag="ACHKUB_004023"
     CDS             complement(3278..4192)
                     /locus_tag="ACHKUB_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO6656979.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4408..5841
                     /gene="cscA"
                     /locus_tag="ACHKUB_004024"
     CDS             4408..5841
                     /gene="cscA"
                     /locus_tag="ACHKUB_004024"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO6656980.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(5849..6844)
                     /locus_tag="ACHKUB_004025"
     CDS             complement(5849..6844)
                     /locus_tag="ACHKUB_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6656981.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLGAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            7075..7293
                     /locus_tag="ACHKUB_004026"
                     /pseudo
     CDS             7075..7293
                     /locus_tag="ACHKUB_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7311..8639
                     /gene="dsdA"
                     /locus_tag="ACHKUB_004027"
     CDS             7311..8639
                     /gene="dsdA"
                     /locus_tag="ACHKUB_004027"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO6656982.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(8747..10285)
                     /gene="emrY"
                     /locus_tag="ACHKUB_004028"
     CDS             complement(8747..10285)
                     /gene="emrY"
                     /locus_tag="ACHKUB_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO6656983.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(10285..>11419)
                     /gene="emrK"
                     /locus_tag="ACHKUB_004029"
     CDS             complement(10285..>11419)
                     /gene="emrK"
                     /locus_tag="ACHKUB_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO6656984.1"
                     /translation="SNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNA
                     DPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLY
                     LQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAA
                     IQAYKANKALIMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGE
                     TVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGIN
                     MGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNED
                     IAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3269 a         2758 c         2529 g         2863 t
ORIGIN      
        1 atcgtactca taattgccat acggatagca aatgcgaata ttctcatgta gatcggggtc
       61 atccacctca gctccagaac aactttttga actaccggaa gtataccgat acggtgcaac
      121 ataagacgat gtctctccag gcaaaaaata agttagtgtt gtaaggggta taatcagaaa
      181 aaatccagca aatatgcaca ttcctgcata aaccttaagg tatgctgaca gactcttcaa
      241 gccgctttgt tttactatcc ccttcttaac ccaaaacaga gataacagaa aagctattcc
      301 catgctaaac agaatgtaat agtgggatat actctgatta agaaacgtga ccctgtaaat
      361 atctgcccgc caccagaaga aaatgaaaat aaagatcagg cctgaaactg tcatgcaaat
      421 caaataagga tacgaatctt ttttcatgtt tagcgcccat aaaatttttc ctgccccgga
      481 caaatttacc atccattttc tgcgcagaaa atagctcatt acttactgca caataataca
      541 caaaattgcg taaatttttt gcatggattt tagctctttc agccgacgtt taaggggtaa
      601 atagcatttc ctaaaagcaa ctgcaccaac ccaacagaat gagctaccgc ttacgttgag
      661 agcaaaaaag tgtatagcag caatgaacag catcctcgca ctgacgggga tttcttttat
      721 ctgagctcgc tacggcgagt tttgttttat ggagacaaga aatgtcagat ttggctatga
      781 aggttttgaa atggcaatca actggcgatg tcggcatcag tagcgcaact cttgcctcaa
      841 tcgcatgtgg cctgaaaaag aatatctatg gtcatcactt cggcgctccc catgacgcag
      901 cagactttcg gcgatgcgtt gcacttgttg agcagattcc agaaatcaga gattcattcg
      961 acaaggttgc aaagcgcgtt ccggcattca aaggaatcct caacgaatgg gattcactcg
     1021 ttgctctgtt gaagtctgaa atgaagacgt acgggaacaa agcaccagag acttacagaa
     1081 gaatcagcga gctacgcaag gactaacccg cctcacactc gatgaggcct gttcatttct
     1141 caagatatcc agacctacca ttgctgcatc aatgcggctt tttattgcct gatttgcagg
     1201 ttcgattcca tattcggaga tagcactcat gcaacacgaa ctacaacctg attcactggt
     1261 tgatttgaaa ttcatcatgg ctgatactgg cttcggtaag accttcatct atgaccggat
     1321 taagtccggc gacctgccta aagccaaagt tatccacgga cgagcaagat ggttatatcg
     1381 tgaccattgt gaattcaaaa ataagctctt aagccgcgcc aatgggtaaa atagcgggta
     1441 aaatattttt cacatctaaa aaacaccatt ccaatcaatc ccctgccgcc tcaagtagat
     1501 gtctgcaggg gacaccagat acccttcaaa cgaaatctac cttcaccccg taaaagatgg
     1561 gtttggcagc acacttgcct tatatctact catttttact gcaacaggtt gaaatctcag
     1621 cactgtcaga aagcgctgat gactaaacag ccctgggccg ggcgatgtaa ccatcacaca
     1681 gaatcctgat agcgaaatat ggcgtgactc gatacttcac tccgcaatgc attccttgat
     1741 gaattcgcag gaccgtgata cacgggacag gtcactgaat gacgacaatg tcctggaaat
     1801 cagcgaaccg cgcatctgaa gtacatttga gcgactgtac cagaacatga atgaggcgtt
     1861 tggattaggc gattattagc agggctaagc attttactat tattattttc cggttgaggg
     1921 atatagagct atcgacaaca accggaaaaa gtttacgtct atattgctga aggtacaggc
     1981 gtttccataa ctatttgctc gcgtttttta ctcaagaaga aaatgccaaa tagcaacatc
     2041 aggcagacaa tacccgaaat tgcgaagaaa actgtctggt agcctgcgtg gtcaaagagt
     2101 atcccagtcg gcgttgaaag cagcacaatc ccaagcgaac tggcaatttg aaaaccaatc
     2161 agaaagatcg tcgacgacag gcgcttatca aagtttgcca cgctgtattt gaagacggat
     2221 atgacacaaa gtggaacctc aatggcatgt aacaacttca ctaatgaaat aatccagggg
     2281 ttaacgaaca gcgcgcagga aaggatacgc aacgccataa tcacaactcc gataagtaat
     2341 gcattttttg gccctacccg attcacaaag aaaggaataa tcgccatgca cagcgcttcg
     2401 agtaccacct ggaatgagtt gagataacca tacaggcgcg ttcctacatc gtgtgattcg
     2461 aataaacctg cataaaagac aggaaaaagt tgttgatcaa aaatgttata gaaagaccac
     2521 gtccccacaa taaatatgac gaaaacccag aagtttcgat ccttgaaaac tgcgataaaa
     2581 tcctcttttt ttacccctcc cgcatctgcc gctacgcact ggtgatcctt atctttaaaa
     2641 cgcatgttga tcatcataaa tacagcgcca aatagcgaga ccaaccagaa gttgatatgg
     2701 ggactgatac taaaaaatat gccggcaaag aacgcgccaa tagcatagcc aaaagatccc
     2761 caggcgcgcg ctgttccata ttcgaaatga aaatttcgcg ccattttttc ggtgaagcta
     2821 tcaagcaaac cgcatcccgc cagatacccc aagccaaaaa atagcgcccc cagaattaga
     2881 cctacagaaa aattgctttg cagtaacggt tcataaacgt aaatcataaa cggtccggtc
     2941 aagaccagga tgaaactcat acaccagatg agcggtttct tcagaccgag tttatcctga
     3001 acgatgccgt agaacatcat aaatagaatg ctggtaaact ggttgaccga ataaagtgta
     3061 cctaattccg tccctgtcaa ccctagatgt cctttcagcc aaatagcgta taacgaccac
     3121 cacagcgacc aggaaataaa aaagagaaat gagtaactgg atgcaaaacg atagtacgca
     3181 tttctgaatg gaatattcag tgccataatt acctgcctgt cgttaaaaaa ttcacgtcct
     3241 atttagagat aagagcgact tcgccgttta cttctcacta ttccagttct tgtcgacatg
     3301 gcagcgctgt cattgcccct ttcgccgtta ctgcaagcgc tccgcaacgt tgagcgagat
     3361 cgataattcg tcgcatttct ctctcatctg tagataatcc cgtagaggac agacctgtga
     3421 gtaacccggc aacgaacgca tctcccgccc ccgtgctatc gacacaattc acagacattc
     3481 cagcaaaatg gtgaacttgt cctcgataac agaccaccac accttctgca cctttagtca
     3541 ccaacagcat ggcgatctca tactcttttg ccagggcgca tatatcccga tcgttctgtg
     3601 tttttccact gataagtcgc cattcttctt ccgagagctt gacgacatcc gccagttgta
     3661 gcgcctgccg caaacacaag cggagcaaat gctcgtcttg ccatagatct tcacgaatat
     3721 taggatcgaa gctgacaaaa cctccggcat gccggatcgc cgtcatcgca gtaaatgcgc
     3781 tggtacgcga aggctcggca gacaacgcaa ttgaacagag atgtaaccat tcgccatgtc
     3841 gccagcaggg caagtctgtc gtctctaaaa aaagatcggc actggggcgg accataaacg
     3901 taaatgaacg ttccccttga tcgttcagat cgacaagcac cgtggatgtc cggtgccatt
     3961 catcttgctt cagatacgtg atatcgactc cctcagttag cagcgttctt tgcattaacg
     4021 caccaaaagg atcatccccc acccgaccta taaacccact tgttccgcct aatctggcga
     4081 ttcccaccgc aacgttagct ggcgcgccgc caggacaagg cagtaggcgc ccgtctgatt
     4141 ctggcaagag atctacgacc gcatccccta aaacccatac tttggctgac atttttttcc
     4201 cttaaattca tctgagttac gcatagtgat aaacctcttt ttcgcaaaat cgtcatggat
     4261 ttactaaaac atgcatattc gatcacaaaa cgtcatagtt aacgttaaca tttgtgatat
     4321 tcatcgcatt tatgaaagta agggacttta tttttataaa agttaacgtt aacaattcac
     4381 caaatttgct taaccaggat gattaaaatg acgcaatctc gattgcatgc ggcgcaaaac
     4441 gccctagcaa aacttcatga gcaccggggt aacactttct atccccattt tcacctcgcg
     4501 cctcctgccg ggtggatgaa cgatccaaac ggcctgatct ggtttaacga tcgttatcac
     4561 gcgttttatc aacatcatcc gatgagcgaa cactgggggc caatgcactg gggacatgcc
     4621 accagcgacg atatgatcca ctggcagcat gagcctattg cgttagcgcc aggagacgat
     4681 aatgacaaag acgggtgttt ttcaggtagt gctgtcgatg acaatggtgt cctctcactt
     4741 atctacaccg gacacgtctg gctcgatggt gcaggtaatg acgatgcaat tcgcgaagta
     4801 caatgtctgg ctaccagtcg ggatggtatt catttcgaga aacagggtgt gatcctcact
     4861 ccaccagaag gaatcatgca cttccgcgat cctaaagtgt ggcgtgaagc cgacacatgg
     4921 tggatggtag tcggggcgaa agatccaggc aacacggggc agatcctgct ttatcgcggc
     4981 agttcgttgc gtgaatggac cttcgatcgc gtactggccc acgctgatgc gggtgaaagc
     5041 tatatgtggg aatgtccgga ctttttcagc cttggcgatc agcattatct gatgttttcc
     5101 ccgcagggaa tgaatgccga gggatacagt taccgaaatc gctttcaaag tggcgtaata
     5161 cccggaatgt ggtcgccagg acgacttttt gcacaatccg ggcattttac tgaacttgat
     5221 aacgggcatg acttttatgc accacaaagc tttttagcga aggatggtcg gcgtattgtt
     5281 atcggctgga tggatatgtg ggaatcgcca atgccctcaa aacgtgaagg atgggcaggc
     5341 tgcatgacgc tggcgcgcga gctatcagag agcaatggca aacttctaca acgcccggtt
     5401 cacgaagctg agtcgttacg ccagcagcat caatctgtct ctccccgcac aatcagcaat
     5461 aaatatgttt tgcaggaaaa cgcgcaagca gttgagattc agttgcagtg ggcgctgaag
     5521 aacagtgatg ccgaacatta cggattacag ctcggcactg gaatgcggct gtatattgat
     5581 aaccaatctg agcgacttgt tttgtggcgg tattacccac acgagaattt agacggctac
     5641 cgtagtattc ccctcccgca gcgtgacacg ctcgccctaa ggatatttat cgatacatca
     5701 tccgtggaag tatttattaa cgacggggaa gcggtgatga gtagtcgaat ctatccgcag
     5761 ccagaagaac gggaactgtc gctttatgcc tcccacggag tggctgtgct gcaacatgga
     5821 gcactctggc tactgggtta acataatatc aggtggaaca acggatcaac agcgggcaag
     5881 ggatccgcgt cactcttccc ccttcacgac cttcaataat atgcaatgca gcttcccgcc
     5941 cgataatgtc atgtggaagc tgaattgtgg tcagcggcgg taaaaacaga tgcccgacgc
     6001 caaccagatt atcaaagccc attacggcga catcctgcgg gattcgtacc cccttcgcca
     6061 gaagaacctg ataagccaca aaggctgcgc gatcgttacc acatatcaga acatcaaaat
     6121 ctggtttgcc cgatttgaag tggtcattga gtaaacttgc gagatcggtg tagtgatcat
     6181 cacctgttgc catgtgaaat tgtttcacct cagccagatc tcgtccagca tcacgccagg
     6241 cctgctcaaa tccctgccga cgataccctg ttgccaacgc actttccggt agccagaagc
     6301 ataacggttg acgatagccc gccccgagca aatgctgtgt tgattcatat tgtgcagtgt
     6361 aatcatcagg gatataactg ggtaacgctg ggtcatccgc cacacagttc gccaatacaa
     6421 tattttcacc atacagagac tcaggcagcg tgatatgtcg cagccccatt gtagtataga
     6481 taatgccatc cggacggtgg gcaagcagct gacgtgccgc gcgggcagcg tcatcttcag
     6541 aaaaaatatt gattaaaaaa ctattccagc cgaactcgct ggcggtttgc tcaatggcaa
     6601 gcagaatatc aacagagaaa ggagtggtag ccgtgtcctg cgccagcacg gcgagagtcg
     6661 acggcttacg tccttgagcg cgcatcttac gggcggaaag atcaggaaca taattcaggg
     6721 tctggattgc ctgcaatacg cggtcacgcg ttgcaggacg cacagattct gcattatgca
     6781 tcacccggga gactgtcatc atcgacactc ccgccaggcg tgcgacatcc tttaatgaag
     6841 ccatacccaa gccgtttgcc gtaaaacggg cactgtagca gaaacagacg tcactggcga
     6901 gatccaacgc cctatcacct gacacagcaa tacaataaaa aataacaata attcccggac
     6961 aattgtcccc aattccgcct ctgttctcgc caacgagtaa ccatataggc aagtaagtaa
     7021 aacatgccaa ctggcatcgc caacctggag aagaaaatat aaatatggat cattgtgctg
     7081 ccgctgtatc ccgacatcag cccggaaatt attgcgattg ctatcggttc aggtgcaatt
     7141 ggctgcacga tcgttacgga ctcgcttttc tggctagtga agcaatattg cggcgctacg
     7201 ctcaatgaaa catttaaata ctatacgaca gcgacattta tcgcttcagt cgtcgctctg
     7261 gcgggcacat tcctgctgtc atttatcatc taagcgcaaa gagacgtact atggaaaacg
     7321 ctaaaatgaa ctcgctcatc gcccagtatc cgttggtaaa ggatctggtt gctcttaaag
     7381 aaaccacctg gtttaatcct agcacgacct cattggctga aggtttacct tatgttggcc
     7441 tgaccgaaca ggatgttcag gacgcccatg cgcgcttatc ccgttttgca ccctatctgg
     7501 caaaagcatt tcctgaaact gctgccactg gggggattat tgaatcagaa ctggttgcca
     7561 ttccagctat gcaaaaacgg ctggaaaaag aatatcagca accgatcagc gggcaactgt
     7621 tactgaaaaa agatagccat ttgcccattt ccggctccat aaaagcacgc ggcgggattt
     7681 atgaagtcct ggcacacgca gaaaaactgg ctctggaagc ggggttgctg acgcttgatg
     7741 atgactacag caaactgctt tctccggagt ttaaacagtt ctttagccaa tacagcattg
     7801 ctgtgggctc aaccggaaat ctggggttat caatcggcat tatgagcgcc cgcattggct
     7861 ttaaggtgac agttcatatg tctgctgatg cccgggcatg gaaaaaagcg aaactgcgca
     7921 gccatggcgt tacggtcgtg gaatatgagc aagattatgg tgttgccgtc gaggaaggac
     7981 gtaaagcagc gcagtctgac ccgaactgtt tctttattga tgacgaaaat tcccgcacgt
     8041 tgttccttgg gtattccgtc gctggccagc gtcttaaagc gcaatttgcc cagcaaggcc
     8101 gtatcgtcga tgctgataac cctctgtttg tctatctgcc gtgtggtgtt ggcggtggtc
     8161 ctggtggcgt cgcattcggg cttaaactgg cgtttggcga tcatgttcac tgcttttttg
     8221 ccgaaccaac gcactcccct tgtatgttgt taggcgtcca tacaggatta cacgatcaga
     8281 tttctgttca ggatattggt atcgacaacc ttaccgcagc ggatggcctt gcagttggtc
     8341 gcgcatcagg ctttgtcggg cgggcaatgg agcgtctgct ggatggcttc tataccctta
     8401 gcgatcaaac catgtatgac atgcttggct ggctggcgca ggaagaaggt attcgtcttg
     8461 aaccttcggc actggcgggt atggccggac ctcagcgcgt gtgtgcatca gtaagttacc
     8521 aacagatgca cggtttcagc gcagaacaat tgcgtaatgc cactcatctg gtgtgggcga
     8581 cgggaggtgg aatggtgccg gaagaagaga tgaatcaata tctggcaaaa ggccgttaat
     8641 aacgtttcaa cgcagcatcg caatcctttc cctgggtgag cgatgctgcc gatggcgcag
     8701 acttaagatc cccggtctta cccgctataa ccccctcctt ttatcatcac ccaatgcctt
     8761 tcgctgtaaa cggcggtttc gcaaaccaaa ccaaaaccgt aagtaagata aaagcaatag
     8821 ccgccatacg gaaaatctca tttgcagaaa tagaaagtga ctgctgggtt atttcattat
     8881 taatttcatt aagaactcct gaaagcgaac cataatattt atccataatt tgtgacgatg
     8941 aattaaacac ggggttaaat tgatcgatgg ttgctgtcaa ctgactatgg tgtaacgatt
     9001 cgcgtcgtcc ccacagcgtc attgtcaacg acgtaccaac tgatcctgac aaggtacgaa
     9061 aaaaattact catactcgag gcattggcaa atttattatc tggcaagcct gaaaacgaaa
     9121 tcgttgttaa gggtaaaaag aaacaggcaa cggcgaatcc ctgaaaaaac tgcggcaaaa
     9181 tgatgcctgt aaaatcaatc gttggcataa atgtcacaga acgccagtaa tagcaaaccg
     9241 catacatcaa aaaactaaat gtcactaaca accgcatgtc tattttgttg ccataacgtc
     9301 ctatcaaagg tgaaattaat agtggcatga tgccgatggg cgcataagca agcccggccc
     9361 atatcgcatt ataccccatc gtttcctgga gtaactgcgg cataaggacg atcgctccag
     9421 agtaaaataa atacgcgcag gtgatactca caataccaat ggtgaaatta cgagacttaa
     9481 acaaactgag atcaagaatc ggattctctg aggtcgactc ccaaatgact aaagagatca
     9541 gagaaataac tgatactact gttaatatta ttattgtact cgagttgaac caatccagat
     9601 cgcgcccttt atcaagcata atttgcaagc caccaacacc gagcactaac agggtcagtc
     9661 ctggtagatt catttttacc ggtgaagttt cagtttctct tcctttaagt aaggttaagc
     9721 ataatgtcag gacgataatc cccataggga cattgattaa aaatatccaa ccccagctaa
     9781 agttatcgca aatataaccg cccaatatcg gcccacatat cggagcgata atcacggtca
     9841 ttgaccataa tgccagagca aatgttcttt tttctggtgg ataattcctc aataataaac
     9901 tctgtgacag tggaattaac ggccccgcca ttaatccctg aacgactcta aaaaatatca
     9961 gcacatcaag attggtcgat aaactacaca ttaaggaaga cagcgaaaaa aaagtgactg
    10021 aaagtaaaaa taaccttaat tcgcctattc tttgtgccag cctgccggta acagggatcg
    10081 caatggcatt tgctacgcca aacgaggtga taacccaggt gccttcgtct gttgatgctc
    10141 ccagaaagcc agatattgtc ggtattgcga cgttagaaat agtggaatcc aacatttgca
    10201 taaatgtcgc taatgacaat gcaatagtga cgcaccataa cgtcccaccg gttaatggtg
    10261 ccggagttga tttagtgatt gccattaaag ttgtccatta tgcgaaataa tgttgctaat
    10321 ttctttttct atcggactgg tatcgataac taaagcctta ctggtataag ccggcatgga
    10381 ggtcacggtt gaagccagct caggcatctc ggcaatgtct tcgttcttcg tatcaatagt
    10441 tgctgtcatc gataaaccaa tacgcaaggg atgttccatg agttcttttg gatcaagaga
    10501 aacttcaacc ggtacacgct gaacgatttt gatccagttc cctgtcgcat tttgtgcagg
    10561 taataaggag aacgcattgc cggttcccat attgatccct gtcacccgac catgaaacac
    10621 aacattttca ccataaagat cgctgataat attgaccgat tgaccaatcc gtacatccgt
    10681 aagttgtgtt tctttaaagt tggcattaac ccacatttga cgtgccggta cgacagccat
    10741 taacgattgt ccggggctca ctgtttcgcc gacctgaaca cttctctggg caatatagcc
    10801 ggtaaccgga ctcttaatat ccgtacgttt aagcgccaac caggcttctt tagttgcatc
    10861 cgccgcttca acgacttgtg gctgacggtt taatggtgtg ttcattatta aagctttatt
    10921 cgctttataa gcctggatag cggcattcaa tgccgctttg ctacttatta acgtatcttt
    10981 ggtatgctcc agcgtttctt ttgaaataac cccctgcttc gctaacggca ctcgacggtt
    11041 ataatcttct aaagattgtt gatactgaat acgtgctgaa gcgacttcgg cactgtattg
    11101 tttatcctgt aagtatagtt tattcgtttg ccgaacaata tttgccagat tatttttagc
    11161 tttattgagt gcgatagtgg catcagtttt atccagtgaa actaaaatgt caccttgtcg
    11221 aacgtagttc gtatctttat gattaacgac agtgacacta cctgagactt gtgcagaaat
    11281 tggatctgca ttccccgtga cataggcgtc atctgtacta atcatgtctt ctaattccat
    11341 tgaccaatag gcataggcac ctgaaaacgc aataaataaa actaccgcca ataaagaaaa
    11401 gtattttctt ctgttagaa
//
LOCUS       DAZFRN010000152        11315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFRN010000152 DAZFRN010000000
VERSION     DAZFRN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.154"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..897)
                     /gene="yihR"
                     /locus_tag="ACHKUB_004030"
     CDS             complement(<1..897)
                     /gene="yihR"
                     /locus_tag="ACHKUB_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO6656985.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQR"
     gene            complement(1035..2276)
                     /gene="yihS"
                     /locus_tag="ACHKUB_004031"
     CDS             complement(1035..2276)
                     /gene="yihS"
                     /locus_tag="ACHKUB_004031"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO6656986.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2293..3171)
                     /gene="yihT"
                     /locus_tag="ACHKUB_004032"
     CDS             complement(2293..3171)
                     /gene="yihT"
                     /locus_tag="ACHKUB_004032"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO6656987.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3195..4091)
                     /gene="yihU"
                     /locus_tag="ACHKUB_004033"
     CDS             complement(3195..4091)
                     /gene="yihU"
                     /locus_tag="ACHKUB_004033"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO6656988.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4259..5155
                     /gene="yihV"
                     /locus_tag="ACHKUB_004034"
     CDS             4259..5155
                     /gene="yihV"
                     /locus_tag="ACHKUB_004034"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO6656989.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5189..5974
                     /gene="csqR"
                     /locus_tag="ACHKUB_004035"
     CDS             5189..5974
                     /gene="csqR"
                     /locus_tag="ACHKUB_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6656990.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6073..6672
                     /gene="yihX"
                     /locus_tag="ACHKUB_004036"
     CDS             6073..6672
                     /gene="yihX"
                     /locus_tag="ACHKUB_004036"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO6656991.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6666..7538
                     /gene="yihY"
                     /locus_tag="ACHKUB_004037"
     CDS             6666..7538
                     /gene="yihY"
                     /locus_tag="ACHKUB_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO6656992.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7535..7972
                     /gene="dtd"
                     /locus_tag="ACHKUB_004038"
     CDS             7535..7972
                     /gene="dtd"
                     /locus_tag="ACHKUB_004038"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO6656993.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8017..8958
                     /gene="fabY"
                     /locus_tag="ACHKUB_004039"
                     /gene_synonym="yiiD"
     CDS             8017..8958
                     /gene="fabY"
                     /locus_tag="ACHKUB_004039"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO6656994.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(9022..9930)
                     /locus_tag="ACHKUB_004040"
     CDS             complement(9022..9930)
                     /locus_tag="ACHKUB_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6656995.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            10159..10470
                     /locus_tag="ACHKUB_004041"
     CDS             10159..10470
                     /locus_tag="ACHKUB_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6656996.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            10471..10761
                     /gene="nadS"
                     /locus_tag="ACHKUB_004042"
     CDS             10471..10761
                     /gene="nadS"
                     /locus_tag="ACHKUB_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO6656997.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
BASE COUNT         2718 a         2808 c         2994 g         2795 t
ORIGIN      
        1 tctctgtccg taaatgttaa aaaacaggcg gtgcggtttt cctggttcca gtaataataa
       61 gtcgatacct gaattaaagg cgttaggcgg gcaactcata ggttcaactg ccagtccctg
      121 acgctgtaat ttttcaccgc tataaatttg tacccagggt tgatcgcaac aaaggctaac
      181 cgaaagcgcc tgttgtggat gggtaatggt tatttcccac agatcattag cggttttaaa
      241 ggtgtggtcg atttttgtcg cggctattgt tttcgcctgc gtgaaattta aatccagctc
      301 atcaacgtgg tgaagcgttg tcggatttac gtgttcatca acggcataaa cctggtttgc
      361 cggaagctgg aacagatatt catcaacaga tgttaagttg caggttaaat aagtatgaat
      421 acccacgcca taaggcgctg cgacagtgcc gatattctgg cttgcgatct caacggaaag
      481 acccgtgtgt gcgttgagcg aataaacaac ttgtgaagcc agcataaacg gatagccgta
      541 actgggcggc agaaaagctg tcagggtaac gctggtggcg gttaattcac taatttgcca
      601 gtctcgccag gcaagcagcc cgtgaatagc ggcttttgaa ctatgttcat taatgggtaa
      661 ctgatattcc tgcccctgat agcggtaaca gccgttggcg atccggttcg gccacggaat
      721 aagcacttta cccagatgcg ccagcggcat ctcttcgggt ttgtgcggga tgactaaatg
      781 acaaccctga aaggttaatt cagccagacc agcaccgacg gtcacaatgg tcgcgtgata
      841 atcccccgcg gcaagtgaaa cggtctgtcc actgcaatgc atattggtga tttgcataca
      901 aggcacctta attaacgaca tacagcctcc cgtgaggctg atgtcaaacc caaaaactgc
      961 accggacggt cccctcgccc ctttggggag agggttaggg tgaggggaaa acccgcaccc
     1021 tattttcaga tcagttattt cgcattaata tccagcagac ccgctgcaac cgctggagcc
     1081 atgccgggcg ctaacgggat acgcgggatc accaggcaat gcagcaggtg ataaatatcc
     1141 tgtttgccgt cccagacttt ggtggtgacc ttattgtccg catccagctc ctgccaccag
     1201 gaaccatttt catagtccat caggtactta atgcagtact cccaccatgt ttgataccag
     1261 gtttcatact ggcgatcgcc agtgacggtg tagagcgcgt aggccgtacc cattgcttcg
     1321 acgataggcc aacgtacacg ttcgcggacc accggttttc cttcccagtc aacggtataa
     1381 acaatcccgt ccgcgccatc gggtgcccag gcatcgcgca cggtggcgtt aaacagacct
     1441 ttggcatctt ctagcagcca tgctggtggt tgttcgcaac gggcttccag ggcagcatgg
     1501 atgtgcagca ttaaacggcc ccattcgatc cagtggcctg gtgtacctcc gaacgcgcgg
     1561 aagcgatgcg ccgggttatc tttgttgtaa tccggcagcg gattccactg ggtatcgaag
     1621 tgttcgttaa cgcgataatg attatttctg gcgacgtcgt ggataatcac ggaagccacg
     1681 cgaatcgcgc gatccagcca ttttttgtcg tgagtgacgt cataaacaat caagaaagct
     1741 tccaccgcgt gcatattggc attgccgccg cggtactctt cggttttgct gaaggcttcg
     1801 tcccaggatt ccaggcacat ctgctcttct tcgctccaga aatatttctc gataatttca
     1861 atggtgtaat cgagcagctt gcgcgcttcc gggtgacccg ttgtgacggc gctggcggca
     1921 cccagcagag caaagaagtg ttgatagccc tgtttggagg catccaccac gccctcgtca
     1981 ttcacgcagg cataccagcc gccatatttt ttatcacgca gtgcgccgtt catggctttg
     2041 ataccgtgat caaccaacga gtaagcgcca ggtcgaccca tcgccgcagc aacggaataa
     2101 acgtgtaaca tacgagcggt gatccacaga tgggtgccca tctcttcttt gatttgccct
     2161 ttattgccta accagccaaa accagtcggc actactgaat ttttaccaaa atcaaagatg
     2221 cggtcggttt cctgttccag ccaacggttg tggcttaggg tgttaaacca tttcattcct
     2281 tttatcctca tcttagcggc gtttagccat catttcgtcg acgatatcgc caagttgttg
     2341 taatttcggt gcgcaaacgt cacgcagcat cagctcgttg tctggtaaac cgacgaccga
     2401 tgcccagact gcacggcctg ccaggaatcc cgatgcgccc gccgtcattg ccacgcgtac
     2461 ggcacgcggg aacagttttt cgtcgacacc ggaagagagg atcacccatg gcatattgat
     2521 atggtcattc agacgttgtg aagcacagag aagctcttgt tgcggacctt tgccataaag
     2581 cggcatttca actttgtaga ggtcagcgcc actgtcgccc agctctttgg cggcatcgat
     2641 gatcgcttgt tcgcgatcga atttatcgcc acgacgcggt gggcggacga ctggctcaat
     2701 gatgcttacc agaccgtgtg agtggcacag ttcgttgaac tctttcacca tatccagacg
     2761 ttgctgcgca tcttcatcac tacgccacag caccagcagt tttaaggctt tgccaccgtc
     2821 ttgtttgatc tgtagcggat tgattttgcg gtcaataacc acgctatcga ccggaatacc
     2881 gttgccagga atgaactcat cggcggcgac aatcatggcg caacttttgg caatcgcgtt
     2941 ttgctcaacc acctggcggt agcagaattg ttgatctacc agaatcgccg aggcataagg
     3001 cgagagggcc tttgcagcgt taactttgaa atcagttaaa acgctatcgg ctaccggagc
     3061 gggtgcccca gccgcggcaa acatcatgcg catggcttcg cgctgatcga ccgccagcat
     3121 ggcaaaaccg cccgatgcgc gcgtaatgtc gttgatggtg tacttattca ttcatttatt
     3181 cctttatcca gtcgttacat ttttactttg gcagtcatcc cggcactgac acggacctgt
     3241 tccagaatgg cggaccagtc ctggcgaccg cgacccgctg cgcgcgcctg gctataaacc
     3301 tcccgtgagg cggcccccag cggcattggc acatgcagct ggttggcgac atcaagggcg
     3361 atgccaagat ccttatgggc aagatcgatc atgaaggcgg gagaaagatc tccgctgagg
     3421 actttgtttg gccaggaagt ggtgaagtga cctttaccgg cggcggtacc gctcatcact
     3481 ttgacggcaa catcgaaggg aagattcagg gcttcgcaca aaacggcggc ttctgccgaa
     3541 agcgcattga gcgcgatgct catgtagttg ttgatgagct taacgcggat ccccatgccc
     3601 ggaccgccag agttgatcaa ctcactgccc atcgccatca ggatcggtgt ggcacgttca
     3661 acttgttcag cggtgccgcc agccagcagt aacagagtac cggtaatggc atttgcagaa
     3721 gtacggccta ccggaacatc catcatgctg aagcctttgg cttgcatatc ggcaatcaat
     3781 ttatcggttt gcagcggatg gatggtggac atatcaatga ccagcgcatc ggtagataag
     3841 ccttcgcaaa cgccgttttc accgaacaac acgttgcgca ccagatcgcc attcggcaac
     3901 atggtaatga taaattcggc atctttagcg gcctgcgccg ggttggcggc gggagtcgca
     3961 cctttgtcta ccagatgccg cacagcttcg gcgttcacat caaagacgcg aagttgatgc
     4021 ccttgctgca ataaattgct cgccattggc gaacccattt gtcctaaacc gataaacgcg
     4081 attgctgcca taaccctctc ctgaatacag tgatgtcact ttttgtcatt tatgacatgc
     4141 tttgcttgtc tgtttttgat cgtatttgta atttatcgtc aaaaaattga cagccgtcac
     4201 tttttaaaca attggtgaaa ttaaaatgaa cgcatcccaa aatgtttaag gaatgaccat
     4261 gattcgtgtt gcttgtgtag gtataaccgt gatggatcgc atctattacg tggaagggtt
     4321 accgacggag agcggtaaat acgtggcgag aaattatacg gaagttggtg gcgggccagc
     4381 ggcgacggca gcggttgcgg cggcaaggct gggggcgcag gtcgatttta ttggtcgcgt
     4441 aggtgatgac gacacgggca acagcctgct ggcagagctg gaatcctggg gcgttaacac
     4501 ccgttacacc aaacggtata accaggcgaa atcttcgcaa tccgccatca tggtggatac
     4561 caaaggcgag cggataatca ttaactaccc cagcccggat ctgctgcctg acgcagagtg
     4621 gttggaggaa attgatttct ctcagtggga tgttgtgctg gcagatgtac gctggcacga
     4681 cggcgctaaa aaagccttca ccctggcccg tcaggcgggt gtgatgaccg ttctggacgg
     4741 ggacattacg ccgcaggata tcagtgagct ggtggcatta agcgatcacg cggccttttc
     4801 agaaccgggt ctggcgcgct taacgggcgt gaaagagatg gccagtgcgc taaaacaggc
     4861 acaaacgctc acaaatggtc atgtctatgt gacccagggt agcgcaggct gcgactggct
     4921 ggaaaatggt gggcgtcagc atcaaccggc cttcaaagtt gatgtggtag ataccacagg
     4981 tgcgggtgat gtttttcacg gcgctttggc ggtggcgctg gcaacaagtg gggatttagc
     5041 ggagtcagtc cgcttcgcca gcggtgtagc ggcgttaaaa tgcacacgtc ccggtggacg
     5101 agccgggatc cctgactgtg atcaaacccg atcttttttg tcactttttg tataaaatgc
     5161 cagggtgatg gtttttcgag gaattttcat gagccttacc gaactaaccg gtaacccgcg
     5221 gcacgatcaa ctcctcatgc tgatcgccga gcgtgggtat atgaatattg atgagctggc
     5281 aaatctgctg gatgtctcca cgcagacggt ccgccgggat attcgtaaat taagcgagca
     5341 aggcctgatt acgcgccatc acggtggcgc gggtcgggct tccagcgtcg ttaatacggc
     5401 gttcgagcag cgtgaggttt cgcaaaccga ggaaaaaaaa gcgattgccg aagcggtggc
     5461 agactatatt cctgatggtt caacaatatt tatcaccatt ggtacgactg ttgagcatgt
     5521 tgcccgggcg ttacttaacc ataatcattt gcggataatc accaacagcc tgcgtgtggc
     5581 gcatattctt taccacaacc cgcgctttga agtgatggtg cccggcggta cgttgcgctc
     5641 tcataatagc gggatcattg gcccttcagc ggcgtccttt gtggctgatt ttcgtgctga
     5701 ttatctggta acaagcgttg gggcgattga gagcgatggc gcgttgatgg agtttgatgt
     5761 aaacgaagct aacgtggtga aaacgatgat ggcgcacgcg agaaatattc tgctggtcgc
     5821 cgatcacact aagtatcatg cttcggcggc ggttgaaatt ggtaacgtgg cacaggtcac
     5881 tgcgctcttt accgacgagc tgccgcccgc tgcgctaaaa tcacgcttac aagacagcca
     5941 aattgaaatc attcttcccc aggaagacgc gtagattttt gtgaccgtta acctggcttc
     6001 ataccttgcc acatagccaa acccatcctt tcccgctaca gttaatttct tgtggcgcga
     6061 aaggaggcaa aaatgctcta tatctttgat ttaggtaatg tgattgtcga tatcgacttt
     6121 aaccgtgtgc tgggagcctg gagcgattta acgcgtattc cgctggcatc gcttaagaag
     6181 agttttcata tgggggaggc gtttcatcag catgagcgtg gggaaattag cgacgaagcg
     6241 ttcgcagagg cgctgtgtca tgagatggct ctaccgctaa gctacgagca gttctctcac
     6301 ggctggcagg cggtgtttgt tgcgctgcgc ccggaagtga tcgccatcat gcataaactg
     6361 cgtgagcagg ggcatcgcgt ggtggtgctt tccaatacca accgcctgca taccaccttc
     6421 tggccggaag aatacccgga aattcgtgat gctgctgacc atatctatct gtcgcaagat
     6481 ctggggatgc gcaaacctga agcacgaatt taccagcatg ttttgcaggc ggaaggtttt
     6541 tcacccagcg atacggtctt tttcgacgat aacgccgata atatagaagg agccaatcag
     6601 ctgggcatta ccagtattct ggtgaaagat aaaaccacca tcccggacta tttcgcgaag
     6661 gtgttatgct aaaaaccatt caggacaaag ccaggcaccg tacccgtcca ctatgggcct
     6721 ggctaaaact actctggcaa cgcattgatg aggacaacat gacaaccctg gcaggtaacc
     6781 ttgcctatgt gtcgttgctc tcattagtgc cgctggttgc cgttgttttt gcgcttttcg
     6841 ccgcttttcc catgttttcc gacgtcagca ttcagttacg tcactttatt tttgccaact
     6901 ttctgcctgc tactggcgat gttattcagc ggtatatcga acaatttgtt gccaattcca
     6961 acaagatgac cgccgttggg gcgtgcgggc tgatcgtcac cgcgttattg ctgatgtact
     7021 ccatcgatag cgcgttgaat accatctggc gcagtaaacg agcgcgacca aaaatttatt
     7081 cgttcgccgt gtactggatg attttaacgc tggggccgct gctggcaggg gccagtctgg
     7141 cgatcagttc ctatttgctc tccctgcgct gggcgagcga tctcaatact gtcatcgata
     7201 acgtgctgcg tatttttccg ctgctgttgt cgtggatctc cttctggttg ctgtacagca
     7261 ttgttcctac catccgcgta cctaaccgcg acgcgattgt cggcgcgttt gtcgccgcgc
     7321 tcctgttcga agcaggaaag aaaggtttcg cgctttatat caccatgttc ccctcatatc
     7381 agctcattta cggcgtgctg gcggtgatcc ccattctctt tgtttgggtc tactggacat
     7441 ggtgtatcgt cttgcttggc gcggaaatta ctgtcactct cggggaatac cgcaaactaa
     7501 aacaagcagc tgaacaagaa gaagacgacg aaccatgatt gcattaattc aacgcgtaac
     7561 ccgtgccagc gtcaccgtgg agggagaagt gacgggcgaa attggcgcgg gacttttggt
     7621 gttattgggt gtcgaaaagg atgacgacga acagaaagca aaccgtctgt gcgagcgtgt
     7681 gctcggctac cgcatcttta gcgatgccga aggcaagatg aacctcaacg tgcaacaggc
     7741 gggcggcagt gtgctggtgg tttcccagtt taccctcgcc gcagataccg aacgggggat
     7801 gcgcccaagt ttctccaaag gtgcatcacc ggatcgcgca gaggcgttat atgactattt
     7861 cgtcgaacgc tgccgtcagc aagagatgaa cacgcaaaca ggacgcttcg ctgcggatat
     7921 gcaggtatcg ctggtcaatg atggccccgt gacattctgg ttgcaggtat gagccagctt
     7981 ccagggttgt cacgggaaac aagagagagt atcgctatgt atcaccttcg ggttccacaa
     8041 acagaagaag aattagagcg ttactatcag tttcgctggg aaatgttgcg taagcccctg
     8101 catcaaccaa aaggttcgga acgcgacgcg tgggatgcga tggcgcatca ccagatggtc
     8161 gtcgacgagc agggtaatct ggtggcggta ggccgattgt atattaatgc cgacaatgaa
     8221 gcgtccattc gctttatggc cgttcacccg gacgtgcagg acaaagggtt aggcacgctg
     8281 atggcgatga ccctggagtc ggtggcgcgt caggaaggcg ttaagcgcgt gacctgtagc
     8341 gcccgtgaag acgcggtgga gtttttcgcc aagctgggat tcattaatca gggggagatc
     8401 accaccccaa ctaccacgcc gattcgccat tttttgatga ttaaacccgt tgccactctg
     8461 gatgatattt tgcatcgcgg cgactggtgc gcgcagctgc aacaggcgtg gtacgaacat
     8521 atcccgctta gtgaaaaaat gggcgtgcgt attcaacaat ataccgggca aaaatttatc
     8581 accaccatgc cggaaaccgg taatcagaat ccgcaccata cgctgtttgc cgggagttta
     8641 ttctcactgg caacgctcac cggttggggg cttatctggc tgatgctgcg cgaacgccat
     8701 ctcggcggaa cgattattct ggcggatgcg catatccgct acagcaagcc gattagcggt
     8761 aaacctcatg cggtagccga ccttggtgcc ttaagcggcg atctcgaccg tctggcgcgc
     8821 ggacgaaaag cacgggtgca gatgcaagtt gaaatctttg gcgacgagac gccgggtgca
     8881 gtgtttgaag gcacgtatat cgttctgccc gcgaagccat ttggcccgta tgaagagggc
     8941 gggaacgaag aagagtaggg gcgttttact tgactgaaaa ggttttcaat ctgaaaacct
     9001 tttcagggcc caggtccgaa gttaccttcc cgctctccag gcaacatccc gtagtgccga
     9061 tcgcgcggcc tcgctgatac cacctaactg aaaaaatccg tggataacgc caaggtagcg
     9121 ttggcaggtg cattgcactc cttgccctgt catgcggtga tacaacgctt caccttcatc
     9181 gcaaagtggg tcgtattcag cggtaatgat atgtacaggt ggcaagccat tgaaatcatc
     9241 gcgccaaaga gggctggctt caggatgcct gcgttcaatg cctggcagat acatctcaaa
     9301 gccgctcagt aaagtgtcgc gtgtgatgat gtaatcgtga ccattgcgta tgtagctttc
     9361 gaaatgcgca gtcgcatcaa gcataggata aattaagatg agttgtgccg gttgccactc
     9421 gccagcacgc tttaatcgca aagcagttac cagcgccaga tgtccgcccg cactgtcacc
     9481 ggcaagagtt atacgttgtt tatcaacgcc gagtcgttcg gcgtgttgcc agactatttc
     9541 tgcacctcgt tgcgcatcat catgggcggc ggggaaagta tgttccggtg ccagccgata
     9601 ctggacggca ataacccgac attgcccgta ataggctaac tggcgcagct ggttgtcgtg
     9661 agttgcgaac ccgccgctaa caaaacagcc accgtagtaa taaattatcg tcgggagtaa
     9721 tgtcggggca ttcagcggcg acactattcg taaggtcatc ccttcaagtt cgatagtctg
     9781 aatatctacc cgcgtttctg tctcacccgc aaggacagcg ctggcgatat agccctccct
     9841 gcgctgcgca atactttgtt gacgcgatga ggggcgaccc gcagcgataa acttttcgac
     9901 taactctgca attccttttt ccagtgccat agtagaactt attttgctgt atgaatgtac
     9961 agtcttatac acccgttttt gctggatgga tacagggaaa ttcacgggcg tgaaaaatct
    10021 ggaaagcgcc tcgcaaatct aaagacaaac ccggcagaga cgtggttttg ctggagcact
    10081 atcagtaagc tagtggtttc aagggtacgt caatgacgta caaaaaggat tgttactcta
    10141 agccaatggc gtataattat gctcttcatc gaaacggaaa tttttactga agatgttcaa
    10201 aaactgctga ccgacgatga attcagtcgt tttcagtttt tcctggcctt gaatcctgac
    10261 tatggtgagg taatacctga aactggtggg ttaagaaagg ttcgatgggt ttcaggagga
    10321 aaggggaaac gtgctggtgt cagggttatt tactttcatc aggttaaaca ttatgagatc
    10381 agattactgc ttatttatcg taagggtatt aaagatgatc tgtctcccca ggaaaaagca
    10441 atgctccggt tgttaaatac gaggtggtaa atggataagg cactttttga gcggttgact
    10501 cacagcatgg cccagatgaa tgaaattatt gaaggaacgc gacagccttc gcggactttt
    10561 gaagttgatg ctatgaaaat caaagaaata cgccgcgctt cagggttgtc acaatctaaa
    10621 tttgccgatc ttatttctgt aagcgtagac acgttgcgta actgggagca ggggcgacgt
    10681 tcaccgaccg ggcctgcaaa ggctttgctg cgagcgattg ccaatgatcc gcaacatgtg
    10741 ctgcaagcac ttaaccgtta gaggcatggt tatgccatac agaaagcact agcattcaga
    10801 actcctgtga aagcggcata gtggtcgcct gcccaggagt tttgctatga cccgtaatcg
    10861 tgctgaacgt cgccacccta tgatgcgatt aaaaaaactc atcgtcgcgc tgatgattga
    10921 cgtcaatgct atcggatgat accggggggt tccggaagga attccttgtt tcccgaactg
    10981 ttaccaacaa aattataacg atgaacaagt aaagaccggt tggtggcagt actgaccggt
    11041 ctgtgatgga tgattaattt caccttaaat ccgggtgctg ttaggatgct gacatttttc
    11101 gggtacacca ttcagattat gcaaataaag atccaatcaa aaaataaaca tccctgaata
    11161 taatatattc atagcatcca taactcatcc tgggtgataa ctaaaccatt tttgagtaat
    11221 aaatgaaaca actgcaacac tcttattatt tctggaaaac acctcgcaaa aacaaaacaa
    11281 catgcaaaaa tccgcttttt ttaaatgatt ttccg
//
LOCUS       DAZFRN010000153        11273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFRN010000153 DAZFRN010000000
VERSION     DAZFRN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.155"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            184..1755
                     /gene="garD"
                     /locus_tag="ACHKUB_004043"
     CDS             184..1755
                     /gene="garD"
                     /locus_tag="ACHKUB_004043"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO6656998.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1904..2239
                     /gene="prlF"
                     /locus_tag="ACHKUB_004044"
     CDS             1904..2239
                     /gene="prlF"
                     /locus_tag="ACHKUB_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO6656999.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2239..2703
                     /gene="yhaV"
                     /locus_tag="ACHKUB_004045"
     CDS             2239..2703
                     /gene="yhaV"
                     /locus_tag="ACHKUB_004045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO6657000.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2758..3567)
                     /gene="agaR"
                     /locus_tag="ACHKUB_004046"
     CDS             complement(2758..3567)
                     /gene="agaR"
                     /locus_tag="ACHKUB_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO6657001.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3816..5096
                     /gene="kbaZ"
                     /locus_tag="ACHKUB_004047"
     CDS             3816..5096
                     /gene="kbaZ"
                     /locus_tag="ACHKUB_004047"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO6657002.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            5119..5592
                     /gene="agaV"
                     /locus_tag="ACHKUB_004048"
     CDS             5119..5592
                     /gene="agaV"
                     /locus_tag="ACHKUB_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO6657003.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5603..6382
                     /gene="agaW"
                     /locus_tag="ACHKUB_004049"
     CDS             5603..6382
                     /gene="agaW"
                     /locus_tag="ACHKUB_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO6657004.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSMMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6372..7250
                     /gene="agaE"
                     /locus_tag="ACHKUB_004050"
     CDS             6372..7250
                     /gene="agaE"
                     /locus_tag="ACHKUB_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO6657005.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVIHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7268..7702
                     /gene="agaF"
                     /locus_tag="ACHKUB_004051"
     CDS             7268..7702
                     /gene="agaF"
                     /locus_tag="ACHKUB_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO6657006.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7699..8832
                     /gene="nagA"
                     /locus_tag="ACHKUB_004052"
     CDS             7699..8832
                     /gene="nagA"
                     /locus_tag="ACHKUB_004052"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6657007.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9183..10337
                     /locus_tag="ACHKUB_004053"
     CDS             9183..10337
                     /locus_tag="ACHKUB_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO6657008.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10350..11210
                     /gene="kbaY"
                     /locus_tag="ACHKUB_004054"
     CDS             10350..11210
                     /gene="kbaY"
                     /locus_tag="ACHKUB_004054"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO6657009.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2698 a         2924 c         2980 g         2671 t
ORIGIN      
        1 cgcgctaaag cacagatttg cgctttacct tgccgggcgg cactgcaatc cctgaaatga
       61 ttgacattga tcacatttct gcgtttaaac tcctgacatt cttatttcac ccaatgaagt
      121 catttatttt taaatgagac caggtcctca ttttaataac ccctggctgg agaatatagt
      181 acaatggcca acatcgaaat cagacaagaa acgccaactg cgttttatat aaaagttcac
      241 gacacagata atgtggcaat tattgttaat gataatggcc tgaaagcagg aacgcgtttt
      301 ccggatggac tggaattaat tgaacatatt ccccaggggc ataaagtcgc attgctggac
      361 attccggcta atggtgaaat tattcgttat ggcgaagtga ttggttacgc cgtgcgtgca
      421 atcccacgcg gaagctggat cgacgaatca atggttgtac taccggaagc gccgccgtta
      481 cacacgctgc cactggcaac caaagtcccg gcccccttac cgccgctgga aggatacacc
      541 tttgagggct atcgcaatgc cgatggcagc gtgggcacca aaaacctgct cggcatcacc
      601 accagcgtcc actgtgtggc aggcgtggtg gactatgtag taaaaatcat tgaacgcgat
      661 ctgctaccga aatacccgaa cgtcgatggc gtggtggggc tgaatcattt gtacggctgt
      721 ggcgtggcga ttaacgcacc ggcggcagtt gtgcctattc gtaccatcca caatatttcg
      781 ctgaacccta actttggcgg cgaagtaatg gtgattggtc tgggttgtga aaagttgcag
      841 cctgagcgcc tgctggtcgg cacggatgat gtgcaagcta ttccagtaga aagcgccagc
      901 attgtcagtt tgcaggatga aaagcatgtc ggttttcagt ccatggtcga ggatattttg
      961 caggtcgccg aacgccatct acaaaaactg aatcaacgtc agcgagaaac ctgtccggct
     1021 tcagaactgg ttgtcggcat gcagtgcggt ggcagcgatg ccttttctgg cgtaacggca
     1081 aacccggcgg ttggctatgc gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttc
     1141 tcagaagtca cggaagtgcg tgacgcgatc catctgctga caccacgcgc agtgaacgaa
     1201 gaggtcggca aacggctgct ggaggagatg gagtggtacg ataactatct caatatggga
     1261 aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct ggcaaacgtg
     1321 gtagagaagg cactcggctc cattgctaaa tcgggtaaaa gcgcaattgt tgaagtgctg
     1381 tcgcccggtc aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc agccagcgat
     1441 tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg tgcaagtgtt tacgaccggt
     1501 cgtggtacgc cgtacggcct gatggcggta cccgtcatta aaatggcgac ccgcaccgag
     1561 ctggcgaacc gctggtttga tttaatggat attaacgcgg gcactatcgc caccggcgaa
     1621 gaaaccattg aagaggtggg ctggaagttg ttccacttta ttctcgacgt cgccagcggg
     1681 aagaagaaaa ccttctcgga tcaatggggg ctgcataacc agctggcagt gtttaacccg
     1741 gcaccggtga cctgatttct tttggaacgt ccctcgcaaa acatggcctt agtgccatgt
     1801 ttttattgtt taaagccccc acgtccatta ataatgcatt tgcattacct tagttcaagc
     1861 ttataatttg agcagaaaac aggctgtaaa aggacagtga atcatgcccg ctaatgctcg
     1921 ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa caactatccc
     1981 cgcgccagtg cgtgaggcct taaaactgaa gccaggccag gacagcattc attacgaaat
     2041 tctgcctggt gggcaagtct ttatgtgccg actgggagat gaacaggagg atcatactat
     2101 gaatgcattt ttgcgttttc tggatgcaga tatccagaac aacccgcaaa aaactcgtcc
     2161 attcaacatt caacaaggaa agaaacttgt cgctggcatg gacgtcaaca ttgatgatga
     2221 gattggcgac gacgaataat ggattttcca caaagggtta atggttgggc gctatatgct
     2281 catccctgtt ttcaggaaac ctacgacgct ttagttgccg aagtcgagac attaaaggga
     2341 aaagatcctg aaaattatca gagaaaagcc gccacaaagt tattggcggt agtccataaa
     2401 gtgattgagg agcatatcac ggtcaatcca tcatcaccgg cattccgtca tggcaagtcg
     2461 ttaggctctg ggaaaaataa agactggtca cgggtaaaat ttggtgctgg tcgttatcgt
     2521 ctcttctttc gttatagtga aaaagagaaa gtcatcattc tgggatggat gaacgatgaa
     2581 aacactctgc gcacctacgg taaaaaaaca gatgcctata ccgtattcag caaaatgtta
     2641 aaaagaggac atcctcctgc cgactgggaa agcctcaccc aagagacaga agaaaaccat
     2701 tgatggtgtt cacaatgccc gccgaacgta ttcgtgttga ccagcgggca ccctttctca
     2761 ctccccgacc agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg
     2821 aatgccttca tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact
     2881 ggaacgattg aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat
     2941 ccgacggttt aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat
     3001 cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg
     3061 atcgccgtaa aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac
     3121 gccttccgct tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc
     3181 agtgtgctta cgcatcagac gagcaatctc aaaagtggta gtcccggaat cgaggatcac
     3241 ccgatgacca ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt
     3301 gttcagcgcg cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat
     3361 caacgcgcca ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt
     3421 gcggatcgtc acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc
     3481 ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg ctggtgccag tcactcgctt
     3541 ctcacctgaa gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt
     3601 cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttgc ccattgacgc
     3661 aagctactct cgtttcagtg actttcatta tgtttctttt gtgaatcaga tcagaaaact
     3721 attatctttc gttttatttt tatctcacca tgacgcagta tcaactgaaa caaaacgaaa
     3781 gattaatatc gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga
     3841 gacagcacaa agcgggcaaa acaaatggaa tttatgccgt ttgttccgca catccgctgg
     3901 tgctggaggc tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa
     3961 cctccaatca ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg
     4021 gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgttg attctgggtg
     4081 gtgaccatct ggggccaaac cgctggcaaa acctgccggc cgctcaggca atggccaatg
     4141 ccgatgattt gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca
     4201 gcatgtcctg tcaggacgat ccgattccct taactgatga catcgtggct gaacgcgccg
     4261 cccgtctggc gaaagtggcg gaagaaacct gtcttgaaca ctttggcgaa gccgatctgg
     4321 agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc
     4381 tggcggtcac cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg
     4441 aaaagcaagg tttgaatgcc atctggccac gcatcattgc cctggtggtt caacccggcg
     4501 tcgaattcga tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc
     4561 agatggtcga aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc
     4621 cgcaatcgct gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc
     4681 tgaccttcgc cctgcgtgaa gctctgttct ctctggcggc gattgaagaa gaactggtgc
     4741 cagcgaaagc ctgttctggt ctgcgtcagg tgctggaaga cgtgatgctc gaccgcccgg
     4801 aatactggca aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttata
     4861 gctactcgga tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc
     4921 atctggttcg taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc
     4981 cgctgcaata cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta
     5041 tcaaccatat tcaggacatc ctggcgcagt actacacagc ctgtgaaggc caataagcaa
     5101 aacaaagagg aacacgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt
     5161 cacggtcagg tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc
     5221 aacgatgagg ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa
     5281 gggatcgccg tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc
     5341 gatcgacaga aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa
     5401 ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa
     5461 caaatcgcca aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa
     5521 gccgctgggg tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt
     5581 aaattacttt gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat
     5641 tatcgccttt atcgctggcc tggatatgtt taacggccta acacatatgc accgcccggt
     5701 ggtcctcggc ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg
     5761 cggtacgctg gaactggtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa
     5821 cgtgattatc ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga
     5881 tgtcgcggtc ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct
     5941 gttctcgatg atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac
     6001 tcgcggcatt gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct
     6061 ctgcgctttc ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct
     6121 gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt
     6181 tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca tcctgggctt
     6241 tgttgccgca gcctggctca agttaccggt gctggctatt gctgccgctg cgctggcgat
     6301 ggcgctgatc gacctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga
     6361 ggaatttgaa gatggcatct aatcaaacca ccctgccgaa cgtcagtgaa aacgaagaaa
     6421 cattgctgac tggcgtcaat gaaaacgtgt atgaagatca gagcattggc gcggagctga
     6481 cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact
     6541 acgaacgtat gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc
     6601 acactaataa acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc
     6661 atccgtttct ggtgacattt gttatcggca ttatccttgc gatggagcgt tctaagcagg
     6721 acgttaacag tattcagagc accaaaattg ccgtcggtgc gccgctcggc gggattggcg
     6781 atgcaatgtt ctggctaacg ctactgccga tttgtggcgg gataggcgca agccttgcgc
     6841 tacaaggctc tattcttggc gcagtcgtat ttattgtgct gttcaacgtg atacacctgg
     6901 ggctgcgttt tggtctggcg cattatgctt accgcatggg cgtggcggcg attccactca
     6961 ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtctat cgttgggatg acggtaatcg
     7021 gcgcgctggt ggcaacctat gttcgtttaa gcaccacgct ggaaatcaca gcgggcgacg
     7081 cagtggttaa gttacagact gatgttattg acaaactgat gcctgccttc ttaccgctgg
     7141 tctacacctt gaccatgttc tggctggtac gccgcggctg gagtccgctg cgcctgattg
     7201 cagttaccgt ggttctcggc atcgtcggta aattctgcca tttcctttaa atacaatgag
     7261 gtattagatg ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa
     7321 agcaatgaag caaatcctcg gcgagcaatc gcagtttatt gccatcgatt ttcccgaaac
     7381 ctcaagcacc gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga
     7441 agacggcatt gtttttctga ctgatttgct gggcggcacg ccgtttcgcg tagcttcaac
     7501 gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc accaatttgc aactgctact
     7561 ggagatggtg ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga
     7621 gtgtggacac cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg
     7681 tccggtcgag gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag
     7741 ggatggctcg atgaccatca gttacgtatt gctgacggtg tcatcgcagc aatcgaaccg
     7801 attccagtgg gcgtgactga acgcgatgcg gaactgctct gccccgctta catcgacacc
     7861 catgtacacg gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag
     7921 ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg
     7981 ccgcttaata ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga
     8041 cctggtgcgc aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa
     8101 ggcgcgcatc cgccggaatt gtttcgcgag cttgaaattg ccgagctgga tcaattgatt
     8161 gccgtttctc agcacacctt acgcgtggta gcgctggcac cggaaaaaga gggggcattg
     8221 caggccatcc gccatcttaa acagcaaaat gtacgagtga tgctggggca tagcgcggcg
     8281 acctggcaac aaactcgcgc cgcgtttgat gctggtgccg acggcctggt gcattgctat
     8341 aacgggatga caggtttaca tcaccgcgaa ccgggaatgg ttggcgcggg attaacggac
     8401 aagcgcgcct ggctggaact gatagccgat ggtcatcatg tgcatccggc ggcaatgtcg
     8461 ctgtgttgtt gctgtgcgaa agagagaatc gtactgatca ccgacgcgat gcaggcagct
     8521 gggatgccgg atggtcgcta tacgttatgt ggtgaagaag tgcagatgca cggtggcgtt
     8581 gtccgtaccg cgtctggtgg gctggcgggc agtacgctgt ctgttgatgc ggcagtgcgc
     8641 aatatggtcg agttgacggg cgtaacgcct gcggaagcca tccatatggc gtcgctgcat
     8701 ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc ttaaaccggg caaacgcgcc
     8761 agcgtcgttg cgctggatag cgggctacat gtgcaacaaa tctggattca gggtcaatta
     8821 gcttcgtttt gatggtttgc tccattattg ggccttcact tcccccgtaa ggcctttctt
     8881 tttctttctt tttgatctgt gcagcggtgt cggatgcgac gctaacgcgt cttatgcgac
     8941 ctacggtacg ccgcatccgg caatcggtga acatagcgac gtcattacgc aattctttaa
     9001 tcttcccttg tcttttcttt caacgatcac agatttcgtt ttatttcctt tttcttcatt
     9061 gaactttcag tttcttttct atagatttta atcaacgaaa gacatcacca agtgaaatga
     9121 aacgaaaggc aagtgaaagc gacaacgccc gacgtcaagt tcatcagact aaggattgag
     9181 ttatgccaga aaattacacc cctgctgccg ccgcaaccgg tacatggact gaagaagaga
     9241 tccgccatca gcctcgcgca tggatccgtt cactcaccaa catcgacgcg ctacgttccg
     9301 cgctcaataa cttccttgaa ccgttactgc gcaaagagaa tctgcggatc atcctgaccg
     9361 gagccggaac gtcggcattt atcggtgaca tcatcgcgcc gtggctcgcc agccataccg
     9421 gtaaaaactt cagcgccgta ccgaccaccg atctggtcac caatccgatg gactacctga
     9481 acccagccca tccgctgctg ttgatctcct tcggtcgatc cggcaacagc ccggaaagcg
     9541 tcgctgccgt ggaactggca aatcaatttg taccagaatg ctatcacctg ccgatcacct
     9601 gcaacgaagc gggcgctctt taccaaaacg cgatcaacag cgataacgcg tttgccctgc
     9661 tgatgcccgc agaaacgcac gatcgcggct ttgcgatgac cagcagcatt accaccatga
     9721 tggccagctg cctcgcggtt ttcgcacctg agacgatcaa cagccaaacc ttccgcgacg
     9781 tggcggatcg ttgccaggcg atcctgacct cactgggcga tttcagcgaa ggtgtgtttg
     9841 gttacgcacc gtggaaacgg atcgtttatc tcggcagcgg tggcttacag ggcgcagcac
     9901 gcgagtcggc gctgaaagtg ctggaactga ctgcgggtaa actggcggcc ttctatgatt
     9961 ccccgaccgg attccgtcat ggcccgaaat cactggtcga taacgaaacg ctggtggtgg
    10021 tatttgtctc aagccaccct tacacccgtc agtatgatct tgatctgctg gctgaactcc
    10081 gccgtgacaa ccaggcaatg cgcgtaatcg ccatcgccgc ggaaagcacc gacatcgtcg
    10141 ctgccggtcc acatattatc ctgccgccgt cacgtcactt tatcgacgtt gagcaggcat
    10201 tttgcttcct gatgtacgcc cagacgtttg cactgatgca gtcgctgcac atgggcaata
    10261 cgccggatac cccatcagcc agcggcaccg ttaaccgcgt ggtgcaaggc gtaatcattc
    10321 atccgtggca ggcataagag gatcgcatta tgagcattat ctccactaaa tatctgttac
    10381 aggacgccca ggccaatggc tacgcggtgc ctgcttttaa cattcataac gccgagacga
    10441 tccaggcgat cctcgaagtg tgcagtgaaa tgcgatcgcc ggtgatcctc gccggaacgc
    10501 cggggacctt taaacatatc gcgctggaag agatctacgc cctgtgcagc gcctattcca
    10561 caacctacaa catgccactg gcgctgcatc tcgaccacca cgaatcgctg gatgatattc
    10621 gccgtaaagt ccacgcaggt gtgcgcagtg caatgatcga cggcagccac ttcccgtttg
    10681 ccgagaacgt gaagctggtg aaatcggttg ttgacttctg ccactcacag gattgcagcg
    10741 tggaagcaga actgggccgc ctgggcggtg ttgaagatga catgagcgtt gacgccgaaa
    10801 gtgcattcct gaccgatcca caagaagcta aacgctttgt cgaactgact ggcgtcgaca
    10861 gcctggcggt agcgattggt acggcgcacg gcttatacag caaaacgccg aagattgatt
    10921 tccagcggct ggcggaaatt cgtgaagtgg tggatgttcc tctggtgcta catggtgcca
    10981 gcgatgttcc ggatgaattt gtccgtcgca ctattgaact tggcgtcaca aaagtgaacg
    11041 ttgccacaga attaaaaata gccttcgctg gcgcggttaa agcctggttt gcggaaaatc
    11101 cgcagggtaa tgatcctcgt tattatatgc gcgtcggaat ggatgcgatg aaagaagttg
    11161 tcagaaataa aattaatgtc tgtggttcag cgaatcgaat ttcagcataa tcattagttt
    11221 tttatgattt atcccaatgt acttcccgat taatatcggg gagtgcctta atg
//
LOCUS       DAZFRN010000154        11141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFRN010000154 DAZFRN010000000
VERSION     DAZFRN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.156"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(36..1865)
                     /gene="glmS"
                     /locus_tag="ACHKUB_004055"
     CDS             complement(36..1865)
                     /gene="glmS"
                     /locus_tag="ACHKUB_004055"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO6657010.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2027..3397)
                     /gene="glmU"
                     /locus_tag="ACHKUB_004056"
     CDS             complement(2027..3397)
                     /gene="glmU"
                     /locus_tag="ACHKUB_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO6657011.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3748..4167)
                     /locus_tag="ACHKUB_004057"
     CDS             complement(3748..4167)
                     /locus_tag="ACHKUB_004057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO6657012.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4188..5570)
                     /gene="atpD"
                     /locus_tag="ACHKUB_004058"
     CDS             complement(4188..5570)
                     /gene="atpD"
                     /locus_tag="ACHKUB_004058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO6657013.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5597..6460)
                     /gene="atpG"
                     /locus_tag="ACHKUB_004059"
     CDS             complement(5597..6460)
                     /gene="atpG"
                     /locus_tag="ACHKUB_004059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO6657014.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6511..8052)
                     /gene="atpA"
                     /locus_tag="ACHKUB_004060"
     CDS             complement(6511..8052)
                     /gene="atpA"
                     /locus_tag="ACHKUB_004060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO6657015.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8065..8598)
                     /gene="atpH"
                     /locus_tag="ACHKUB_004061"
     CDS             complement(8065..8598)
                     /gene="atpH"
                     /locus_tag="ACHKUB_004061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO6657016.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8613..9083)
                     /gene="atpF"
                     /locus_tag="ACHKUB_004062"
     CDS             complement(8613..9083)
                     /gene="atpF"
                     /locus_tag="ACHKUB_004062"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO6657017.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9145..9384)
                     /gene="atpE"
                     /locus_tag="ACHKUB_004063"
     CDS             complement(9145..9384)
                     /gene="atpE"
                     /locus_tag="ACHKUB_004063"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO6657018.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9431..10246)
                     /gene="atpB"
                     /locus_tag="ACHKUB_004064"
     CDS             complement(9431..10246)
                     /gene="atpB"
                     /locus_tag="ACHKUB_004064"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO6657019.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10255..10635)
                     /gene="atpI"
                     /locus_tag="ACHKUB_004065"
     CDS             complement(10255..10635)
                     /gene="atpI"
                     /locus_tag="ACHKUB_004065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO6657020.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2664 a         3037 c         2746 g         2694 t
ORIGIN      
        1 accggtcggc agcccttttt tatctatgat agtgattact caaccgtaac cgattttgcc
       61 aggttacgcg gctggtcaac gtcggtgcct ttgatcagcg cgacatggta agccagcagc
      121 tgcagcggaa cggtgtagaa gatcggtgca atcacctctt ccacatgcgg catctcgatg
      181 atgtgcatgt tatcgctact tacaaaaccc gcatcctgat cggcgaagac atacaactga
      241 ccgccacgcg cgcgaacttc ttcaatgttg gatttcagtt tttccaacag ttcgttgttc
      301 ggtgcaacga cgataaccgg catatccgcg tcaatcagcg ccagcgggcc gtgtttcagc
      361 tcgcctgcag cgtaggcttc agcgtgaatg taagagatct ctttcagctt caatgcgcct
      421 tccagcgcga ttgggtactg atcgccacgg cccaggaaca gcgcgtgatg tttgtcagag
      481 aaatcttctg ccagagcttc aatgcgtttg tcctgagaca gcatctgctc aatacggctc
      541 ggcagcgcct gcagaccatg cacaatgtca tgttcaatgg aggcatccag acctttcagg
      601 cgagacagct tcgccaccag catcaacagc acagttaact gagtggtgaa tgctttagtg
      661 gatgccacgc cgatttccgt acccgcgttg gtcatcagcg ccagatcgga ttcgcgcacc
      721 agagaagaac ccggaacgtt acagattgcc agcgaaccaa ggtaacccag ctctttcgac
      781 agacgcaggc ccgccagggt atccgcggtt tcgccagact gtgacaaggt gatcatcagg
      841 ctgttacgac gcacggcaga tttgcgatag cggaattcag aggcgatttc gacgtcgcac
      901 ggaatacctg ccagcgattc aaaccagtaa cgggaaacca taccggagtt ataagaagta
      961 ccacaggcga ggatctgaat atgctcaacc ttcgacagca gttcgtcggc gttcggtccc
     1021 agctcgctta aatcaacctg accgtggctg atgcgcccgg taagggtgtt tttgatcgcg
     1081 ttcggctgtt cgtagatctc tttctgcatg tagtgacggt aaataccttt atcgcccgcg
     1141 tcatattgca gattggattc gatatcctga cgttttactt ccgcgccagt tttatcgaag
     1201 atgtttaccg aacggcgagt aatttccgca atatcgccct cttcaaggaa gataaagcga
     1261 cgggtcaccg gcaacagcgc cagctggtca gaagcgataa agttttcgcc catacccagg
     1321 ccaatcacca gcggactacc agaacgtgcc gccagcaggg tatccgggtg acgggagtcc
     1381 atgatcactg taccgtacgc accacgcagt tgcgggatag cacgcagaac agcctcacgc
     1441 agagtaccgc cctgtttcag ctcccagttc accagatggg caattacttc ggtgtctgtt
     1501 tcagaaacga aggtatagcc acgcgctttt aactcttcgc gcagcggttc atggttttcg
     1561 atgatgccgt tatgcaccac cacaatgtgt tcagaaacat gcggatgcgc attcacttct
     1621 gaaggttcac cgtgggtcgc ccagcgagta tgagcaatac ccgtaccgcc atgcagagga
     1681 tgttcttccg ctgcctgagc cagcatctgg actttaccga ggcgacgcag gcgggtcata
     1741 tgaccttccg catcaacaac ggccagaccg gcagagtcat atccgcggta ttccagacga
     1801 cgtaaacctt caagaaggat ttctgctaca tcacgttgcg cgatcgcgcc aacaattcca
     1861 cacatagttt ttgattccga tttatatcgt tgtcggtcaa cctgtatgcc cgttttttcg
     1921 ggcgccccga gccttgtaga gagtggggtt atttttatag ttactgcttg tgggagggag
     1981 attatgttat ctcctcatcc catgtgaccg gattacccgg ccagaatcac tttttcttta
     2041 ccggacgacg ccagccttct ttctgagtct gcggcacacg gctgatagct aatgcatttt
     2101 cgccgacatt acgcgtcaca gttgtacccg cagcaatggt cgcgcctttg cctactgtta
     2161 ccggggccac cagctgagtg tcggaaccga caaacacatc gtcgccgata atggtcttaa
     2221 atttattcgc accatcgtag ttgcaggtaa tggttcccgc gccgatgtta acgttatcgc
     2281 caatttccgc atcgcccagg taagtcagat gaccagcttt cgagccttta cccagacgcg
     2341 cttttttcat ctcaacaaag ttaccgacgt gtgcaccttc cagcaactca gcaccaggac
     2401 gcagtcgggc aaacgggcca atagtacagg ccgctgccag attcgcgtct tccacaacgg
     2461 tatacggact gatttcgcaa tcatcaccaa tcacgctgtt tttaatcacg caaccggtgc
     2521 cgattttcac gcgatgaccg agagtcacgt tgccctcgat gataacgtta gtatcaattt
     2581 caacatcgcg cccgtgagta agcgtaccgc gcaaatcaaa acgcgccgga tcgcgcagca
     2641 taacgcctgc taacagcagt ttttcagcct gttcggactg gtaaacgcgc tccagacggg
     2701 agagttgcag gcggttattc acaccttcta cttcgcttaa acgttgcgga tgaacggcga
     2761 cgatttcacg cccttcctga tacgccagcg caataatgtc ggtgatgtag tattcgccct
     2821 gagcattatt gttggtcagc ttcgccagcc agcgtttcat atctgcgccg ttagcgatca
     2881 gaatgccggt gttgatctcc tgaatctgac gctgctcgtc ggtggcatct ttgtgctcaa
     2941 caatgccggt aactttgcca ttttcacggg tgatacgtcc ataaccggtc ggatcatcca
     3001 gtttcaccgt cagcagacca atgccaccct gcggtttagc atcacgcagg cgctggagtg
     3061 tttcgacaga gatcagcggc acgtcgccgt agagcattaa aatgtcttca tcatcggcaa
     3121 agaaaggtgc ggcctgctgc attgcatgac ccgtacccag ctgctctgcc tgaagcaccc
     3181 agttaaggtt gtcgtctttc agcgcctgtt taagcagatc gccgccgtga ccgtacacca
     3241 ggtgaacgtg cgctgcgcct aattcattcg cagcatcaat gacatgctga accatcgctt
     3301 tcccggcaag ggtatgcagc actttcggaa gatcggaata catgcgcgtg cctttgcctg
     3361 cggcaaggat cactacgctc atagcattat tcaacatacg cgtcctgact gtaatttgag
     3421 aacgaattta aaccgcttca ccttgaaaaa actacatttt tttcatcgtg aaatggacag
     3481 aggataaatt gttcaataac ggattatccc cgctgacgaa agtgccattt tcgaccatcg
     3541 tcacgccttt tgtctgcaga aaattaaggc agtaccttca tttaagcgtc aggtggatgt
     3601 ttttgctctt atttcgatca atgaataaac gggaaaacag gggtgtttta tatcagcagg
     3661 atctatgtga acgctcttca ggacggatca cgcgtaaaaa aaagccagcc tgtttccaga
     3721 ctggcttttg tgcttttcaa gccggtgtta catcgctttt ttggtcaact cgataacgcg
     3781 cagctgcgcg atcgctttgg ccagttccgc agacgcctga gcgtaatcta cgtcgccgtg
     3841 agagctgcta atgtgctctt cagccttacg tttcgcttcc atggctcgcg cttcgtcgag
     3901 atcctggccg cgaattgcgg tgtcggccag aacggtcacg ttgccaggct gcacttcaag
     3961 aatgccgcca gacagataga taaactcttc gtgaccgtgc tgtttcacga tgcgaatcat
     4021 accaggctta atggcggtga gcagcggtgc gtggccaggg tagatcccca gttcaccttc
     4081 gctacccgtt acctggattt tctcgaccag accagagaac atttgttgct ctgcgctgac
     4141 gacgtccagg tggtaagtca ttgccatatc accctccgat taaggcgtta aagttttttg
     4201 gctttttcca cagcttcttc gatggaaccg accatgtaga acgcctgctc cggcaggtga
     4261 tcgtattcgc cttccatgat gcctttaaag ccacggatgg tgtctttcag ggagacgtat
     4321 ttacccggag aaccggtgaa tacttctgcc acgaagaacg gctgggacag gaagcgctgg
     4381 atcttacgag cacgcgctac caccagtttg tcttcttcag acagttcatc catacccagg
     4441 atggcgatga tgtctttcag ttcctgataa cgttgcagga tggactgaac gccacgcgcg
     4501 gtgtcgtagt gttcctgacc aaccaccagc gggtccagct gacggctggt ggagtccagc
     4561 gggtcaacgg ccgggtagat acccagagac gcgatctgac ggctcagtac cacggttgcg
     4621 tcaaggtgcg caaaggtggt tgccggagac gggtcagtca agtcatccgc aggtacgtat
     4681 accgcctgta cggaggtgat agaaccagtt ttggtggagg tgatacgttc ctgcagaacg
     4741 cccatctctt ccgccagggt cggctgataa cctaccgctg aaggcatacg gcccagcagt
     4801 gcggatactt ccgtaccggc cagggtgtaa cgatagatgt tgtcaacgaa cagcagaacg
     4861 tcacgacctt cgtcacggaa tttctcagcc atggtcagac cggtcagagc aacgcgcaga
     4921 cggtttcccg gcggctcgtt catctggcca tacaccaggg atactttgtc gataacgttg
     4981 gagtcggtca tttcgtggta gaagtcgtta ccctcacgag tacgttcacc tacgcccgca
     5041 aacacagagt aaccggagtg ctcgatcgcg atgttacgaa tgagctccat catgtttacg
     5101 gttttaccta cacccgcacc accgaacaga ccaactttac cgcccttagc gaacggacac
     5161 atcaggtcga taactttgat accggtttcc agcagttcct gagagtttga cagctcttcg
     5221 taggaaggtg ctgcgcggtg aatcgcccaa cgctcttctt caccgatctc gcctttcatg
     5281 tcgaccggtt cacccagtac gttcatgata cggcccagag tcgctttacc taccgggact
     5341 tcgatcgggt gttcgaggtc ttttacatcc agaccgcgac gcagaccgtc ggaggaaccc
     5401 attgcgatgg tacgcacgat accgccgccg agctgctgct gaacttccag caccagacgc
     5461 tcattaccat tttgcacctc aagagcatcg tacacgcgcg gtacggcatc ctgagggaat
     5521 tcgacgtcaa ctacggcgcc gattacctgg acaatctttc cagtagccat cttaaatcct
     5581 ctacgaaata acctgtttaa accgcggcgg cccccgagac gatctcggtg agttcctgag
     5641 taatgctggc ctgacgagct ttgttgtata ccaactgcag ctctttaatc aggctgccgc
     5701 cattgtcggt cgcggctttc atcgccacca tacgggcggc ctgctcgctg gccaggtttt
     5761 caaccacgcc ctgataaacc tgagattcga cataacgacg cagcagggta tccagcaacg
     5821 ccttcggatc gggttcgtac aggtaatccc aggatttatg tttcagatca tcatcatctg
     5881 atgccggtaa cggcagcagc tggctgatgg tcggaacctg agacatggtg ttaataaatt
     5941 tgttgctgac aatgtaaagc ttgtccagac ggccttcgtc gtaggcctgc aacatcactt
     6001 ttaccggacc gatcagttcg gacagggaag ggttatcccc catgccggtg acctgggcaa
     6061 caacattgcc gcccacggag ttgaagaacg acacgccttt cgagccgatc attgcgaggt
     6121 cgcattgaac gcctttgtcg gtccaggtct tcatttccgc cagcagtttt ttgaacaggt
     6181 taatgttcaa accaccgcac aaaccacggt cggtcgacac caccaggtag cccacgcgtt
     6241 taacgtcgcg gtcttccagg taagggtgct tatattccag attaccgtgt gcaaggtgac
     6301 caatcacttt gcgcatggtt tctgcataag gacggctggc cgccatgcga tcctgcgatt
     6361 tacgcatttt ggaagcggcg accatctcca tcgctttagt gatcttttgc gtgttctgga
     6421 cgcttgcgat cttactacgt atctcttttg cgccggccat gagcttctcc tcaatgcctt
     6481 gcggcctgcc ctaaggcaag ccgccagacg ttaccaggat tgggttgctt tgaaggaatc
     6541 gaggatgcct ttcagcttgc cttcgatttc gtcgttgtag ccaccggtct ggttgatctc
     6601 ttgcatcaac ggagcgtgat cacggtcgac gtaagccagc agagcggctt cgaagctgcc
     6661 aattttcgac agttcaacat ccgccaggta accacgttct gctgcgaaca gaaccagaga
     6721 ctgctgcgca acggacatcg gcgcatactg tttctgtttc agcagttcgg tcactttctg
     6781 accgtggtca agctgcttac gtgttgcatc gtcaaggtcg gatgcaaact gagagaacgc
     6841 tgccagttca cgatactgtg ccagagcggt acggatacca ccggacagtt ttttcatgat
     6901 cttggtctgt gctgcaccac caacacggga tacggaaata cccgggttaa ccgcaggacg
     6961 aataccggcg ttgaacaggt tggtttccag gaagatctga ccatcggtaa tggagattac
     7021 gttggtcgga acgaacgcag aaacgtcacc cgcctgagtt tcgataatcg gcagtgcggt
     7081 cagagaaccg gttttccctt tcacttcacc tttggtgaag gcttcaacgt attcggcgtt
     7141 aacacgtgca gcacgctcca gcagacgaga gtggaggtag aaaacgtcgc ccgggaatgc
     7201 ttcacgtcct ggcggacgac ggagcagcag ggagatctga cggtaagcaa cagcctgttt
     7261 agacaggtca tcgtaaatga tcagcgcatc ttcaccgcgg tcacggaagt attcgcccat
     7321 tgcgcaaccg gcatacggtg ccaggtattg cagtgcagcg gattcagacg cggttgctac
     7381 cacaacgatg gtgttagcca gtgcgccgtg ctcttccagt ttacgtacca cgttagaaat
     7441 ggtggacgct ttctggccga tagcgacata gatacatttg ataccggaat cgcgctggtt
     7501 gatgatggca tcgatagcca gtgcggtttt acctgtctga cggtcaccga tgatcaattc
     7561 acgctgacca cgaccgattg ggatcatgga gtcaacggct ttataaccgg tctgtaccgg
     7621 ctgatctacg gactgacgtt cgataacgcc cggagcgatt gcttctacag cagagaagcc
     7681 gtcgtgatcc agcggacctt taccgtcgat tggtgcaccc agagtgttaa ccacacggcc
     7741 cagcaggcca cggccaaccg gaacttccag gatacggcca gtacacttaa ctttcatgcc
     7801 ttcggcaagg tcagcgtacg gacccataac aaccgcacct acagagtcgc gctcgaggtt
     7861 cagtgcgata gcgtaacggt tacccggcag ggagatcatt tcaccctgca tacaatcggc
     7921 caggccgtga atgcggataa caccgtcact tacagaaaca atagtacctt cgttgtgagc
     7981 ttcactcaca acattgaact gagcaatgcg ctgcttgatc agttcgctga tttcggtgga
     8041 attcagttgc atgctccagt ccccttaaga ctgcaagacg tctgcaaggc gctcaagacg
     8101 accgcgtacg ctgccatcaa tgaccatatc acccgctcgg atgataacgc ctgccattac
     8161 agacttatcg attttgcaat tcagcttaac tttgcgtgac agacgttttt ccatcgcagc
     8221 agaaattttc gcgagctgtt gttcactcag tgcggcagcg gaaatgacgt ctacctcagc
     8281 ggtagcctca ctcacggcac gaaggtgaat aaactgctcc agaacatccg ggagcgcgtt
     8341 aagacgacca ttttcagcca ttacccgaat caggttctga ccgttttcgt ccagttgctc
     8401 accacaaact gcgataaacg actcggcgag cgtttctggc gcaagcgcgc cagagagaag
     8461 ctctgccatt tgttcgtttt tggttacctc ggcggcaaac gccagcatgt cctgccagcg
     8521 ttctacactt tggtgttcga cggcaaagtc aaaagctgct ttggcgtagg ggcgagctac
     8581 cgtaataaat tcagacatca gcccctccct ccttacagtt cagcgacaag tttatccacg
     8641 atgtcgctgt tagcagcttc atccacggaa cgttcgatga tcttctcggc gccagcaaca
     8701 gccaggatag caacttgctt acgcagctct tcacgggcac gtttacgctc ggcttcaatt
     8761 tctgcctgcg cctgggccac gattttagta cgttcctgtt ctgcctcagc tttcgcttcg
     8821 tccagaatct gcgagcggcg tttgttcgcc tgctcgatga ttacctgggc ttccgctttc
     8881 gcttttttca gctggtcggt cgcgctggcc tttgcaaggt caaggtcctt atgtgctcgt
     8941 tctgcggaag caaggccgtc agcaatttct ttttgacgtt tttcgatggc tgccattaat
     9001 ggcggccata cgtacttcat gcagaacaga acgaacagga caaacgcgat ggcctggccg
     9061 aggattgttg cgttaagatt cacagcacaa tgcctctatt tagttaacgt tctgatattg
     9121 ctctttaaat aaaagcaacg cttactacgc gacagcgaac atcacgtaca gacccagacc
     9181 tacagcgatc atcgggatag catccaccag acccataacg ataaagaact gagtacgcag
     9241 cagaggaatc agatcaggtt gacgcgctgc gccttccagg aatttacccc cgaggatgcc
     9301 gataccgatc gcagcaccga ttgccgccag acccatcatc acagcggcag ccatgtacag
     9361 cagatccata ttcaggtttt ccatgacagt ctccagtttg tttcagttaa aacgtagtag
     9421 tgttggtaaa ttaatgttct tcagacgcca tcgacagata gacgatcgtc agaaccatga
     9481 agatgaaggc ttgcagcgta atgatcagga tgtggaaaat ggcccacggc acattcagga
     9541 tccactgtga ccaccacggc aacagaccag caatcagaat gaaaatcagc tcaccggcat
     9601 acatgttacc gaacagtcgc aaaccaagtg aaactggttt ggacagcagg cttacccctt
     9661 caaggattaa gttgacagga atgaacgccc agtgattgaa cggctgcagc gtcaactctt
     9721 tcgtgaagcc gccgatgcct ttcattttga tgctgtagaa cagaatcagg ataaatacgc
     9781 ccaatgccat agacagcgtt acgttcacgt ccgcagacgg aaccacacgc agtgcaggca
     9841 gacccagtac atgttcagca atgtacggca gcaggtcgat aggcagtaaa tccatcaggt
     9901 tcatcaggaa tacccagacg aagatcgtca gggccagcgg agcaatcagc ttgcttttgc
     9961 catggtacat gtctttcacg ctaccattaa caaagccgat caccagctca atcgcggtct
    10021 gaaacttacc tggcacaccg ctggtcgcct ttttggctac gctacggaat aaaaccagga
    10081 acaacagacc cagcaccacc gagaagaaca tggagtcaat attgattgtc cagaaggtgg
    10141 ctggggggtt ttgtggatcc accagcgaga atgtacgcag gtccagctga aggttattca
    10201 ggtggtgtcc tatgtaatcc tgcggcgtca tattttctga agccatgatg ccttttaccc
    10261 tttgttgtta attacagccg gtgccagtat ctgaaccacc agcaccaaaa cccacgtaac
    10321 gatcagcggc aagaataccg cctttaaaac cgccaacgcc accaccagta acaccaacat
    10381 cgccagaact ttgaaagctt cgccaaatgc gaatgtccag gccacccggc ctttcgctgg
    10441 tgtatgcgcc tggtgacgcc aggcaaatat cataaacaaa acgttaggca gaaagactgc
    10501 caggcccccg cttattgcag agacgcccca gaaggggtct ttgaggctga acagcaatcc
    10561 acttgctatc accaccagta actgaacgag cagaagcttc cgagcaacgt ttcgactcac
    10621 gagcgacaca gacatcacgt ttttcactcc tgctcccttc gaggtatgcc gcgtgtcgta
    10681 taaaactttc tttaaggctt agagtcaagc atcaaaaagc ggtcaaatta tacggtgcgc
    10741 ccccgtgatt tcaaacaata agtagccaaa aggtgaataa atgtttaaat atttttccag
    10801 tgcatacaat tgcgactttt ctgctaaccc tgttcgatca tgaaaaactg taaataacgc
    10861 gtaaacactg gtgataaagc gtgcttcaga tcacatattg cgcatgttcg cgcacagcat
    10921 atttatttac ttggcaaatg atgcctttgc aagtttatga tatttcagtc taaaaacaga
    10981 tactgtttta ataaatgaca tttacacaac aaaaaccacc cattgacatt tttaataatg
    11041 ttttaacagc caatgatggt tcttagcgcc gatttttagc agactgatat tttcactaat
    11101 gacttatttt ctgcttacca aaaaaagcca cgttatcttg t
//
LOCUS       DAZFRN010000155        11128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFRN010000155 DAZFRN010000000
VERSION     DAZFRN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.157"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..108
                     /locus_tag="ACHKUB_004066"
     CDS             <1..108
                     /locus_tag="ACHKUB_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6657021.1"
                     /translation="FLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            87..191
                     /gene="speFL"
                     /locus_tag="ACHKUB_004067"
     CDS             87..191
                     /gene="speFL"
                     /locus_tag="ACHKUB_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO6657022.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            502..2700
                     /gene="speF"
                     /locus_tag="ACHKUB_004068"
     CDS             502..2700
                     /gene="speF"
                     /locus_tag="ACHKUB_004068"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO6657023.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2697..4016
                     /gene="potE"
                     /locus_tag="ACHKUB_004069"
     CDS             2697..4016
                     /gene="potE"
                     /locus_tag="ACHKUB_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO6657024.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4072..5712)
                     /gene="pgm"
                     /locus_tag="ACHKUB_004070"
     CDS             complement(4072..5712)
                     /gene="pgm"
                     /locus_tag="ACHKUB_004070"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO6657025.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5738..6283)
                     /gene="seqA"
                     /locus_tag="ACHKUB_004071"
     CDS             complement(5738..6283)
                     /gene="seqA"
                     /locus_tag="ACHKUB_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO6657026.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6468..7232
                     /gene="ybfF"
                     /locus_tag="ACHKUB_004072"
     CDS             6468..7232
                     /gene="ybfF"
                     /locus_tag="ACHKUB_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6657027.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7372..7665
                     /gene="ybfE"
                     /locus_tag="ACHKUB_004073"
     CDS             7372..7665
                     /gene="ybfE"
                     /locus_tag="ACHKUB_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO6657028.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7805..8335
                     /gene="fldA"
                     /locus_tag="ACHKUB_004074"
     CDS             7805..8335
                     /gene="fldA"
                     /locus_tag="ACHKUB_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO6657029.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8545..8631
                     /gene="uof"
                     /locus_tag="ACHKUB_004075"
     CDS             8545..8631
                     /gene="uof"
                     /locus_tag="ACHKUB_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO6657030.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8624..9070
                     /gene="fur"
                     /locus_tag="ACHKUB_004076"
     CDS             8624..9070
                     /gene="fur"
                     /locus_tag="ACHKUB_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO6657031.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9154..9480)
                     /gene="chiQ"
                     /locus_tag="ACHKUB_004077"
     CDS             complement(9154..9480)
                     /gene="chiQ"
                     /locus_tag="ACHKUB_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO6657032.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9530..10936)
                     /gene="chiP"
                     /locus_tag="ACHKUB_004078"
     CDS             complement(9530..10936)
                     /gene="chiP"
                     /locus_tag="ACHKUB_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO6657033.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2685 a         2748 c         2777 g         2918 t
ORIGIN      
        1 tttttgttga tcttcttcac agattatagc catttcatgg atagaataac tctaccttca
       61 actgacacag caagaggtaa aggtaaatgg aaaataataa ccgcttaatg cctcatataa
      121 ggcggacaac ccatatcatg aagtttgccc atcgtaatag ttttgacttt catttcttta
      181 atgcccgcta gtcttctgac taaagggcac cccaacgtac aggtctccct gactttaagc
      241 attacaggtt aatacctgta ttcctcggtg ctcatatact gctaaccctt ttaaactcta
      301 aataattcga gtcgcagcac ttgcaacttg aggtatgacg agtatagcca gttaccgggc
      361 tggtctgggt tattgcatct gcaaaaagca aactactgat ttatttatca gcggtggagc
      421 tttgcttttt ttccggcgtg atcgatttct cctttgagaa attgaggacc tgctattacc
      481 tgaaataaag agatgaacaa aatgtcagaa ttaaaaattg ccgttagtcg ttcttgcccg
      541 gattgttttt ccactcatcg tgcatgcgtg aatatagacg aaagtaatta tattgacgtt
      601 gccgccatta ttttatcagt cagtgatgtt gaacgtggaa aactcgatga aatagacgct
      661 actggctatg acattcctgt ttttattgca acggaaaatg aagaacgtgt acccgcagaa
      721 tatctgccac gtatttctgg tgtcttcgaa cattgcgaat cacgtaaaga gttttatgga
      781 cgtcagttag aaaccgctgc cagccattat gaaacgcaac tgcgcccgcc cttcttccgc
      841 gcactggtcg attatgttaa tcagggcaat agtgcttttg actgccctgg tcatcagggg
      901 ggtgagtttt tccgtcgcca tccggcaggc aatcagttcg tggaatactt tggcgaaatg
      961 ctcttccgct ctgacttgtg taatgctgac gtagcaatgg gcgatctgct gattcatgaa
     1021 ggtgcgccat gtattgcaca acagcatgcg gctaaagtct ttaatgccga taaaacctat
     1081 ttcgttttaa acggtacttc atcttctaat aaagtggtct taaacgcatt gctgacgccg
     1141 ggcgatctgg tgttgtttga ccgcaataac cataaatcta accaccacgg tgcgttgcta
     1201 caggctggcg cgacaccggt ttatctggaa accgcacgta acccgtatgg ttttatcggt
     1261 ggtattgacg ctcactgttt tgaagaaagt tacctgcgtg agctgattac agaagttgca
     1321 ccgcagcggg caaaagaggc tcgtcctttc cgcctcgccg ttattcagct aggcacttac
     1381 gacggtacga tttataacgc ccgtcaggtg gtggataaaa ttggtcatct gtgtgactac
     1441 attctgtttg actccgcatg ggttggctat gagcaattta tcccgatgat ggctgactgt
     1501 tcgccgctgt tgctggatct taatgagaac gatccgggta ttctggttac gcaatccgta
     1561 cataaacagc aggctggttt ttctcaaact tcacaaatcc ataaaaaaga cagccacatc
     1621 aaagggcaac agcgttatgt accgcacaaa cgcatgaaca acgcctttat gatgcacgcc
     1681 tccaccagcc cgttctatcc gctgtttgcc gcactggata tcaacgccaa aatgcatgaa
     1741 ggtgtcagcg gtcgtaatat gtggatggat tgtgtggtaa atggcattaa tgcccgcaaa
     1801 ctgatcctcg ataactgtca gcatattcgt ccgttcgtac ctgaactggt ggatggtaaa
     1861 ccctggcagt cgtatgaaac agcgcaaatt gcggttgatc tgcgcttctt ccagtttgta
     1921 ccaggggaac actggcattc ttttgaaggc tatgcagaga atcaatactt tgtcgatcct
     1981 tgcaaactgt tgctgacaac cccaggtatt gatgcacgta acggcgaata tgaagcgttc
     2041 ggtgtacccg cgacgattct tgctaacttc ctgcgcgaaa atggcgtagt gccggaaaaa
     2101 tgcgatctta actccatcct cttcctgctg actccggcag aagatatggc caagcttcag
     2161 caacttgttg ccctgctggt acgcttcgaa aaactgcttg agtccgacgc gccattagca
     2221 gaagtgctgc cttccatcta caaacagcat gaagagcgct acgccggtta taccctgcgt
     2281 cagttgtgtc aagaaatgca tgatttgtat gcccgtcaca acgtgaaaca actgcaaaaa
     2341 gagatgttcc gtaaggaaca cttcccacgc gtaagcatga atccgcaaga agccaactac
     2401 gcctatttac gcggtgaagt ggagctggtt cgtctgccgg atgcagaagg ccgtatcgct
     2461 gccgaaggtg cgcttcctta tcctccgggt gtgctgtgcg ttgttccggg tgaaatctgg
     2521 ggtggtgctg ttctgcgtta cttcagcgct ctggaagaag ggatcaacct gttgccaggt
     2581 tttgcaccgg agcttcaggg tgtctatatc gaagaacatg atggtcgtaa gcaagtttgg
     2641 tgctatgtca tcaagcctcg tgatgcgcaa agtaccctgt tgaaagggga aaaattatga
     2701 gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac catactgacg atggtcaaca
     2761 tgatgggctc cggtatcatc atgctgccga caaagcttgc cgaagttggg acaatttcaa
     2821 ttatctcctg gctggtgaca gccgtaggct caatggcact ggcatgggca ttcgcaaaat
     2881 gcggtatgtt cagccgtaaa tcaggtggta tgggcggtta tgccgagtat gctttcggta
     2941 agtccggcaa ttttatggcg aactacacct acggtgtctc gctgctgatt gctaacgtcg
     3001 cgattgctat ttcggcggtt ggttacggca ccgaactgct cggcgcgagt ttgtcgccag
     3061 tgcagattgg tcttgcgacc atcggagtgc tgtggatttg taccgtggct aactttggtg
     3121 gtgcgcgcat taccgggcaa atcagtagca ttactgtgtg gggggtcatt attccggtgg
     3181 taggcctgtg cattatcggc tggttctggt ttagcccgac gctttacgtt gattcctgga
     3241 atccgcatca tgcaccgttc ttcagtgcag taggttcttc catcgctatg acgctgtggg
     3301 cttttctcgg tctggagtct gcgtgtgcga atactgatgt agtggaaaac ccggaacgta
     3361 atgtgccaat cgcggtactc ggcggtacgt taggtgcggc ggtgatttat atcgtctcca
     3421 ccaacgtgat tgcagggatt gtgcctaata tggagctggc aaattcaacg gcaccatttg
     3481 gcctggcctt cgcacagatg ttcacaccag aagtgggtaa agtcattatg gcgctgatgg
     3541 tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac cattgcccag gtgtttaaat
     3601 cttcatctga tgaaggctac ttccctaaaa ttttctcccg tgtaaccaaa gtggatgcac
     3661 cggtgcaggg aatgttgacc attgtgatta tccagagcgg gttggcgctg atgacgatta
     3721 gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct ggccgtggtg accaatatca
     3781 ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct aatgtgcccc
     3841 cctcaaaagc gaaagttgca aactttgttg cttttgttgg cgcgatgtat agcttttatg
     3901 cgctgtactc atccggggaa gaagccatgc tgtacggttc catcgtgacc ttccttggtt
     3961 ggacactgta tggtctggta tcaccacgct ttgaactgaa aaataaacac ggttaaaaaa
     4021 gcaaagtaaa aaagggcgat cttgcgaccg cccttttttt attaaatgtg tttacgcgtt
     4081 tttcagaact tcgctaacaa tctcaaccgc ttctttctca atctgcttgc gatgttcttc
     4141 accgaggaag ctttcgcagt agatcttata tgcgtcttcc gtgcctgacg gacgcgcggc
     4201 gaaccagccg ttgtcggtca tcactttcag accgccgata gaagcaccat tgcccggtgc
     4261 cgccgtcagg cgcgcggtga tcgggtcgcc tgcaagggtg ctggcgctca ccatttccgg
     4321 agacagctta gacagcgccg ctttttgtgc ggaagtcgcg gctgcctgca aacggttgta
     4381 gctcggcgca ccaaagcgtt ttgccagttc gttgtagtgt tcctgcgggt tcttaccggt
     4441 gacagcggtg atttccgccg ccagcagaca cataatgatg ccgtctttgt cggtggacca
     4501 cggcgtgccg tcgaaacgca ggaaggaagc ccctgcgctc tcttcgccgc caaagccgaa
     4561 gctgccgtcg aacagaccat caacaaacca tttgaaacct accgggactt ctaccagctt
     4621 acggcccaag tcattgacca cacggtcgat catcgcagaa gaaaccagcg ttttaccgac
     4681 agcaacatct ttgccccact gcggacgatg ctggaacagg tagttgatcg ccaccgccag
     4741 gtagtggttc ggattcatca aacctgccgg agtgacgata ccgtgacggt cataatccgg
     4801 gtcgttagca aacgccagat cgaacttatc acgcagtgcc agcaggcccg ccatcgcaca
     4861 ctcggaggag cagtccatac ggatcgcgcc gtctttatca aggtgcataa agcggaaggt
     4921 ttgatcgacc tgatcgttaa cgatggtcag gttgaggttg taatactcgc caatacgctt
     4981 ccagtattcg ataccggaac cgcccagcgg atcaacgccc agcgtcaggc ccgctttctg
     5041 aattgccgcc atatcaacga tatcggccag accttccaca aacggctgca ccagatcctg
     5101 ctcttttaca tgaccggatg ccatcgcttc gtcgagggag atacgcttca cgcctttcag
     5161 gccatcggcc atcagtgcgt tggccctgtc ttccaccact ttggtgacgt tggtatcagc
     5221 cgggccacca tttggcgggt tgtacttgat accaccatct tccggcgggt tatgggacgg
     5281 tgtaatcacg ataccgtctg ccagcgggcc acctttttta ttgtgaacca ggatggcatt
     5341 ggaaatggca ggtgtcggag tgaagccatt gttttcctgc acaatgacat caacgccgtt
     5401 cgctgccagc acttccagta cggaaatgaa cgcaggctcg gacagggcgt gagtatcttt
     5461 acccacatag caagggccag tgatgccgtt tttcgcacgt tcttcagcaa ttgcctgagc
     5521 gatcgccaga atgtgcggct cgttaaagct gtggcgcgct gcactgccac ggtgaccgga
     5581 agtaccgaat ttcaccgcgt gctccgcatt ccctgcttct ggtttcagta cataatattg
     5641 cgccgtcagt tgggcgacgt taatcaaatc actctgttgt gcaggttggc ctgcacgatt
     5701 gtggattgcc attgctttgt cctttgtctg caacgtttta gatagttccg caaaccttct
     5761 caatcaattc cgccgggaat tgcatcgact gcatgatgtg ttcgatcatg ctgcatttac
     5821 ggccggtgtt ggtgttggtg atcacccaat acggcgtgcc tggcacatgt ttcggcttgg
     5881 tctgattacc atttttcagc agcgtttgtt catctgccgc aaagtaaacg cgtgtacgac
     5941 cgtgcaacga ttccgttgct tcggcaaacg cctgggcgtc aagagaatat agtgtagaca
     6001 acagcagcat aaagcgattg accgctcgct tttgctctgc gtattcatcc gaaagcagaa
     6061 gttcacgcat tgcgcgaacc ttgtctttaa tcgttttgac cggcttcgct tcgacgatag
     6121 caggtgacgc aacgcgaacc tctttcgtca ccggagcagc aggctgtgat gcggcggaaa
     6181 atttcaacat acgccgtaaa atgtcggatg cgctctcgcc gatatgctta gtgtggctgg
     6241 caatatagct gtagagttca tcatcaactt caatcgtttt catcttaatc cagtgcagtg
     6301 tcttagctga atacaagtcg ccaggagtat atgaataaat cccggcagcg gatagcgtca
     6361 agcctggctt gcagaaaaac tcggaataaa ccttatttgt tgcagccctg tggcacaatg
     6421 gtcaacataa taccctaacc cgacaacgcg aaaaaaagaa ctttgccatg aaattgaata
     6481 tccgcgcgca aactgcacaa aaccagcaca ataattctcc catcgttctt gtccatggtc
     6541 tgtttggtag cctcgacaac cttggcgtac tggctcgcga tctggtaaac gatcacaata
     6601 tcatccaggt tgatatgcgt aaccacggtc tttcaccgag agatccggta atgaattacc
     6661 cggcgatggc gcaggatctt gttgatactt tggatgcaca gcagatcgac aaagcaacat
     6721 ttatcggtca ctccatgggc ggtaaagcgg taatggcact tactgcactg gcccccgatc
     6781 gcatcgataa actggtggcg atcgatatcg caccggtcga ctatcacgta cgccgtcatg
     6841 atgagatttt cgcggctatc aacgcggtca gtgaatcgga cgcacaaact cgccagcaag
     6901 cagcagcaat aatgcgccag catcttaatg aagaaggggt gattcagttt ctgttgaaat
     6961 cttttgttga cggggagtgg cgctttaacg tgccggtatt gtgggatcag tatccgcata
     7021 ttgtgggttg ggagaaaatc ccggcatggg atcaccctgc cctgtttatc cctggcggca
     7081 attctccgta tgttagcgag cagtaccgtg atgatttact ggcacaattt ccacaggcac
     7141 gggcgcatgt gattgcaggc gcgggtcact gggtccatgc tgaaaaaccg gatgcggtat
     7201 tacgcgctat ccgtcgctat ctcaatgatt aacgcaactg attaaaaacc cagcgtccgc
     7261 gtcgctttcc gcgcgtgggc gttggcgtga ctcatcgcgc tgatgtatta tggcgcgcta
     7321 tccatccggg cagaggcctg gctgattgtt tcccccgaag tcaccaagat catggccaaa
     7381 gaacaaacgg accgtacgac attagatctg ttcgcgcacg agcgtcgacc gggacgaccg
     7441 aaaactaatc cgctttcgcg cgatgaacag ctgcgtatta ataaacgcaa ccagctaaaa
     7501 cgcgacaaag tacgtggcct taagcgtgtc gaactgaagc tgaacgcgga agctgtcgag
     7561 gcgctgaacg agctggcgga gtcgcgcaat atgagccgta gcgaactgat cgaagagatg
     7621 ttaatgcagc aactggcggc gctgcgtagt cagggcatcg tttaaatttc cactttcatg
     7681 tagcacagtg tgcagtcctg ctcgtttgct gattccgcaa gactgcctgt tctgctatga
     7741 ttgcctttat ccgtgggcaa ttttccaccc ccatttcaat aagtttcaag aggttatttc
     7801 actcatggct atcactggca tctttttcgg cagcgacacc ggtaataccg aaaatatcgc
     7861 aaaaatgatt caaaaacagc ttggtaaaga cgttgccgat gtccatgaca ttgcaaaaag
     7921 cagcaaagaa gatctggaag cttatgacat tctgctgctg ggcatcccaa cctggtatta
     7981 cggcgaagcg cagtgtgact gggatgactt cttcccgact ctcgaagaga ttgatttcaa
     8041 cggcaaactg gttgcgctgt ttggttgtgg tgaccaggaa gattacgccg aatatttctg
     8101 cgacgcattg ggcaccatcc gcgacatcat tgaaccgcgc ggtgcaacca tcgttggtca
     8161 ctggccaact gcgggctatc atttcgaagc atcaaaaggt ctggcagatg acgaccactt
     8221 tgtcggtctg gctatcgacg aagaccgtca gccggaactg accgctgaac gtgtagaaaa
     8281 atgggttaaa cagatttctg aagagttgca tctcgacgaa attcttaatg cctgatgtga
     8341 tgcggcgtag actcatgtct acgccgtatt aatagataat gccaatcaaa ataattgcta
     8401 caaatttgta acttttgctg ttgtacctgt acaatgtccc ggtgttcaag tggccttgcc
     8461 gttgtaaatg taagctgtgc cacgttttta ttaacaatat ttgccaggga cttgtggttt
     8521 tcatttaggc gtggcaattc tataatgata cgcattatct caagagcaaa ttctgtcact
     8581 tcttctaatg aagtgaaccg cttagtaaca ggacagattc cgcatgactg ataacaatac
     8641 cgccctaaag aaagctggcc tgaaagtaac gcttccacgt ttaaaaatcc tggaagttct
     8701 tcaggagccg gacaaccatc acgtcagtgc ggaagattta tacaaacgtc tgatcgatat
     8761 gggtgaagaa attggtctgg ctacggtata tcgcgtactg aaccagtttg acgacgctgg
     8821 tatcgtcacc cgccacaatt ttgaaggcgg taaatccgta tttgaactga cacagcaaca
     8881 tcaccacgat cacctgattt gcctcgactg cggcaaggtt atcgaattta gtgatgattc
     8941 catcgaagcg cgtcagcgtg aaattgccgc aaaacatggc attcgcctga ctaaccacag
     9001 tctctatctt tacggtcact gtgccgaagg cgattgccgc gaagatgagc acgcgcacga
     9061 aggcaaataa gccagcctga acgagaaaag ccaacctgcg ggttggcttt tttatacaag
     9121 ggaaataaga aaagcgagag ttacagctct cacttatttg ttattactgc gtatttcctg
     9181 ccagacctta tcacaatcgg ctttcaccgc ctgatcattt ccggtttgcg ttgcacgcag
     9241 acactgctga taatccagta cgcgtacact ttcctggtcg gcaaactgct gatgttgttt
     9301 ctctttcttc agcacgttta acacgctctg gcaggcttca attttttctg gcgaaccttg
     9361 tgcggtgttg atacaggcgc tgtatgcctc tttcagacga ctgtcttcct gtggcgcggt
     9421 tgattgcgca caagctacca gccccgatgc cattatggcg atgagaatca gttttttcat
     9481 agcaaattcc tcaaacgtgc ggcaggcaca aaacctgccg cgtcgggcat cagaagatgg
     9541 tgaatggtgc gattaccata aatttcacgt cacgctcatc ctggaagatg ttaccgtaac
     9601 cgccgcccca gcttgggatg tcagagtggt tgtcgtattc ggtgaagtgc agtttgaaca
     9661 tcgtgccttt ggcgcgaccg tcctgaatgg tgtagaccgc atccaggctg taggcagact
     9721 cttcaatagt ccggtttttg tcgtagtacg catccggatt gctctgccag gtcgcaggtt
     9781 tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa gcctggaaga ttccagtttt
     9841 tcaggtcata catcgcaccg aagaagaccg ctttttcgcc gttggcgttg aagtcggaac
     9901 ggttatccca ccagatatcc aggcggccgt tggaggaagc gtaggttgga gtcatacgtt
     9961 gcaggaagta tccctgctga ccgtcagcct taacccaggt gccttcgagg cgcaaatcca
    10021 ctacgtcagc cgcccggtaa ccaaaggtca acgcttgcag ccaggcggtg ccgtcataaa
    10081 ggtcgttgac gctgcgatcg tcaactttat cgcgcgtacc atagaactgg tagctggtgg
    10141 ttaacgggct accggcgata tcaaatttgt agctggcttt ggcaaaatat tgatcgatat
    10201 acccttccgc ctgaccaaat gccgcttcca gtacgaagtt atttttgaag tcgtatttcg
    10261 ccccaaggga gtgcagataa tcaactttgg tggttttatc gttctgataa aactcatcca
    10321 tttccagatg ccacggcgct ttgtattcgt tggtccacat gtaggagaaa ctcaaggcac
    10381 cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc ctgataagta cctggcataa
    10441 agctccagtg cggcgctaac agcgtttgac cagttggctg aatgtaacct gccctcgccc
    10501 aaaccggacc gtatttaaat ttggccgcag ctttatacag gcttataccg cttttgtcgc
    10561 cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg ttcgggtggg
    10621 agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc agcaatatca aggccgaaca
    10681 tatcagcagc ataaccggac tgaaaatcga ggttggcatt ccaggtggag tgagaaaggt
    10741 tggttttgta tttgtcgccg tcggtaacat ctttacggtc gcgttcacgc tgccagtaat
    10801 atataccgcc ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc tccggcattg
    10861 ccataaagcc cgacattgct gtaacaccgg cgatagccag cgccagcgta ctacgtttgc
    10921 cactaaacgt acgcatgggt taatcctctt tgacgtataa attgctgcac caaaggtgaa
    10981 cagcgaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga ctacaggaac
    11041 agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg taagaacatg
    11101 agcaaccgca catattttga tgattttt
//
LOCUS       DAZFRN010000156        11094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFRN010000156 DAZFRN010000000
VERSION     DAZFRN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.158"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(33..386)
                     /gene="yjeI"
                     /locus_tag="ACHKUB_004079"
     CDS             complement(33..386)
                     /gene="yjeI"
                     /locus_tag="ACHKUB_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO6657034.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(524..2170)
                     /gene="groL"
                     /locus_tag="ACHKUB_004080"
     CDS             complement(524..2170)
                     /gene="groL"
                     /locus_tag="ACHKUB_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO6657035.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2214..2507)
                     /gene="groS"
                     /locus_tag="ACHKUB_004081"
     CDS             complement(2214..2507)
                     /gene="groS"
                     /locus_tag="ACHKUB_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO6657036.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2783..4039
                     /gene="yjeH"
                     /locus_tag="ACHKUB_004082"
     CDS             2783..4039
                     /gene="yjeH"
                     /locus_tag="ACHKUB_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO6657037.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4055..4531)
                     /gene="fxsA"
                     /locus_tag="ACHKUB_004083"
     CDS             complement(4055..4531)
                     /gene="fxsA"
                     /locus_tag="ACHKUB_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO6657038.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            4868..6304
                     /gene="aspA"
                     /locus_tag="ACHKUB_004084"
     CDS             4868..6304
                     /gene="aspA"
                     /locus_tag="ACHKUB_004084"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO6657039.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6422..7723
                     /gene="dcuA"
                     /locus_tag="ACHKUB_004085"
     CDS             6422..7723
                     /gene="dcuA"
                     /locus_tag="ACHKUB_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO6657040.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7839..8177
                     /gene="cutA"
                     /locus_tag="ACHKUB_004086"
     CDS             7839..8177
                     /gene="cutA"
                     /locus_tag="ACHKUB_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO6657041.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8153..9850
                     /gene="dsbD"
                     /locus_tag="ACHKUB_004087"
     CDS             8153..9850
                     /gene="dsbD"
                     /locus_tag="ACHKUB_004087"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO6657042.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9887..10462
                     /gene="yjdC"
                     /locus_tag="ACHKUB_004088"
     CDS             9887..10462
                     /gene="yjdC"
                     /locus_tag="ACHKUB_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6657043.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            10569..10644
                     /locus_tag="ACHKUB_004089"
     tRNA            10569..10644
                     /locus_tag="ACHKUB_004089"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10602..10604,aa:Phe,seq:gaa)
     gene            10887..11001
                     /locus_tag="ACHKUB_004090"
                     /pseudo
     CDS             10887..11001
                     /locus_tag="ACHKUB_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2607 a         2899 c         2752 g         2836 t
ORIGIN      
        1 tgatacgttt gtcacccagc ctctggatca catcagttcg ggcacttata aacctgaccg
       61 ataatctggc tatccgtcgg gacaaaactg gacaacattc cctgcgacgg gctgctgatg
      121 ccataaatca cgttaccgcc cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg
      181 cgcatagaac cgccctcttc tccgtgttgc ccggaaagcc agttgctttg cttacctgtc
      241 gcagtaccaa tcagctggca ctctgcgcct ggctgctcgt ccacaatgcg tacactctga
      301 ccggcagcac tcaattcgtt gctggagcta caacccgcca tcagtagcgc ggcaccgaca
      361 atccctgcta agtattttac gtgcatgtta ttccccataa tcaatgagct ggacgccact
      421 cgcgtcgtcc gtgtctgaat cttatactag aaagatgacc aaaagaaaaa cccccagaca
      481 tttctgcccg ggggtttgtt tatttctgcg aggtgcaggg caattacatc atgccgccca
      541 tgccacccat gccgcccata ccgccagcag cgcctaagtc agctgcatca tttttcggca
      601 ggtcggtaac catgcactcg gtggtgatca tcaggccagc cacagaagcc gcgtactgca
      661 gagcagaacg ggttactttg gttgggtcca ggatacccat gtcgatcatg ttgccgtatt
      721 cttcggttgc tgcgttgtaa ccgtagttgc cgtcgccgcc tttaacggtg ttagcaacaa
      781 cagacggttc ttcgccgcag ttcaggacga tctgacgcag cggagcttcc attgcacgca
      841 gtgcaacttt gatacccacg ttctggtctt cgttctgacc acgcaggtca gccagtttag
      901 acgcaacgcg gatcagcgca acaccaccac cagcaaccac gccttcttct accgcagcac
      961 gggtcgcgtg cagggcgtct tcaacgcgtg cttttttctc tttcatttca acttcggtag
     1021 cagcacctac tttgataact gcaacgccgc ctgccagttt cgctacgcgt tcctgcagtt
     1081 tttcacggtc gtagtcagaa gttgcttctt caatctgctg acggatctga gcaacacggc
     1141 cctggattgc agcttcttca cccacgccat cgatgatagt ggtggtgtct ttgttgatca
     1201 caacacgttt agcctgaccc aggtcttcca gggttgcttt ttccagctcc ataccgatct
     1261 cttcagagat tacggtaccg ccagtcaggg ttgcgatatc ctgcagcata gctttacgac
     1321 gatcgccgaa gcccggagct ttaaccgcag caactttcac gatgccacgc atggtgttaa
     1381 caaccagagt tgccagcgct tcgccttcaa catcttcagc gatgatcagc agcggtttgc
     1441 ctgctttggc aacagcttcc agaaccggca gcatttcgcg gatgttagag attttcttgt
     1501 cagccagcag gatgaacggg ctttccagtt ctactgcgcc agtttccggc ttgttgatga
     1561 agtaaggaga caggtagcca cggtcgaact gcataccttc aaccacgtcc agttcgtcct
     1621 gcagaccggt accgtcttca acggtgataa cgccttcttt accgactttg tccatcgctt
     1681 cagcgatcag tttacctacg gtttcgtcgg agttagcgga gatggtacca acctgagcaa
     1741 tcgctttaga gtcagagcac ggtacggaca gcgctttcag ttcttcaact gcagcggtaa
     1801 cggctttgtc gataccacgt ttcaggtcca tcgggttcat gcccgcagca acagctttca
     1861 gaccctcagt gatgatagcc tgagccagta cagttgcagt ggtggtaccg tcgcctgcag
     1921 cgtcgttcgc tttagaggca acttctttca ccatctgcgc acccatattt tcgaacttgt
     1981 cttccagttc gatttcacga gcaacggaaa caccatcttt ggtgatggtc ggtgcaccga
     2041 aagatttatc cagaactacg ttacggcctt tcggaccgag ggtaactttc actgcatctg
     2101 ccagtacgtt tacgccgcgc agcattttca cacgagcgtc gttaccgaat tttacgtctt
     2161 tagctgccat tatctttatt ccttaaattc gtatgttcag tgtcgtgcga ggattacgct
     2221 tcaacaattg ccagaatgtc gctttcggac atgatcaaca cttcttcatt gtcgatcttc
     2281 tcagatttca caccgtagcc atcgttgaaa ataacgatgt cgccaacttt cacatccagc
     2341 ggcttcactt cgccattttc aaggatacgg ccattgccga cagccagcac ttcgccgcgg
     2401 gtggatttag ccgctgcaga gccggtcaga acgatgccgc cagcagattt agtttcaact
     2461 tctttacgct tgacgatcac gcgatcatgc aatggacgaa tattcattga taactctcct
     2521 ttgagaaagt ccgtatctgt tatgggtgac gccggagctt acgtggtttc ccggctggtg
     2581 accagagaaa tggggatgag gattcgcccc ttcaaggggg aaaaagaaaa aaaattctga
     2641 tcagcacaaa atcgggtgaa aaccctgatt cacctcacat ttcatcgcaa tttcttcatc
     2701 gccgtcataa gcgaatctga ttgtgctacc atcgaaaatc tacgcagttg cccaaaattt
     2761 gggcgcaatc ggatatcaac ccatgagtgg actcaaacaa gaactggggc tggcccaagg
     2821 cattggcctg ctatcgacgt cattattagg cactggcgtg tttgccgttc ctgcgttagc
     2881 tgcgctggta gcgggcaata acagcctgtg ggcgtggccc gttttgatta tcttagtgtt
     2941 cccgattgcg attgtgtttg cgattctggg tcgccactat cccagcgcag gcggtgtcgc
     3001 gcacttcgtc ggtatggcgt ttggttcgcg acttgagcga gtcaccggct ggttgttttt
     3061 atcggtcatt cccgtgggtt tgcctgccgc gctacaaatt gccgccgggt tcggccaggc
     3121 aatgtttggc tggcatagct ggcaactgtt gttggcagaa ctcggtacgc tggcgctggt
     3181 gtggtatatc ggtactcgcg gtgccagttc cagtgctaat ctacaaaccg ttattgccgg
     3241 acttatcgtc gcgctgattg tcgctatctg gtgggcgggc gatatcaaac ctgcgaatat
     3301 ccccttcccg gcacctggta atatcgaact taccgggtta tttgctgcgt tatcagtgat
     3361 gttctggtgt tttgtcggtc tggaagcatt tgcccatctt gcctcggaat ttaaaaatcc
     3421 agagcgtgat tttcctcgcg cattgatgat tggtctgctg ctggcaggat tagtctactg
     3481 gggctgtacg gtagtcgtct tacacttcga cgcctatggt gaaaaaatgg cggcggcagc
     3541 atcgcttcca aaaattgtag tgcagttgtt cggtgtagga gcgttatgga ttgcctgcgt
     3601 gattggctat ctggcctgct ttgccagtct caacatttat atacagagct tcgcccgcct
     3661 ggtctggtcg caggcgcaac ataatcctga ccactacctg gcacgcctct cttctcgcca
     3721 tatcccgaat aatgccctca atgcggtgct cggctgctgt gtggtgagca cgttggtgat
     3781 tcatgcttta gagatcaatc tggacgctct tattatttat gccaatggca tctttattat
     3841 gatttatctg ttatgcatgc tggcaggctg taaattattg caaggacgtt atcgactact
     3901 ggcggtggtt ggcgggctgt tatgcgttct gttactggca atggtcggct ggaaaagtct
     3961 ctatgcgctg atcatgctgg cggggttatg gctgtttctg ccaaaacgaa aaacgccgga
     4021 aaatggcata accacataat ccggcgtttc gacattaatc ctggcgatcg tctttatgat
     4081 caaggcggtc gcgctcatca tcctttcgct ggtactcacc atcaaaagta ttaccgccac
     4141 cggtcccggc gctaaaaccg ccgcctggca tacgagaaaa gcgcaaatgc ggcatcaact
     4201 tcactgtcag atgcttttgc accggcggta ataaaagcag aaggccgagg aagtcggtga
     4261 aaaagcccgg taataaaagc agcaaaccgg caatgatcag cgaaacactt ttaatcatct
     4321 ccgccgctgg gttttcaccc gccgccattt tttgctgcat cagcacgaaa ttcttaaagc
     4381 cctggttacg taccagtgac atcccgataa ctgacgtgaa tataaccagc acgagggtca
     4441 gcaatacccc caatacatgg gcaacctgaa taaagattga aatctcaata tagacataaa
     4501 ggaaaatggc aataaaaggt aaccagcgca aaggtttctc ctgtaatagc agccggtaaa
     4561 acccggctac ctgaatgggt tgcgaatcgc gttcagctta tattgtggtc attagcaaaa
     4621 tttcaagatg tttgcgcaac tatttttggt agtaatccca aagcggtgat ctatttcaca
     4681 aattaataat taaggggtaa aaaccgacac ttaaagtgat ccagattacg gtagaaatcc
     4741 tcaagcagca tatgatctcg ggtattcggt cgatgcaggg gataatcgtc ggtcgaaaaa
     4801 cattcgaaac cacatatatt ctgtgtgttt aaagcaaatc attggcagct tgaaaaagaa
     4861 ggttcacatg tcaaacaaca ttcgtatcga agaagatctg ttgggtacca gggaagttcc
     4921 agctgatgcc tactatggtg ttcacactct gagagcgatt gaaaacttct atatcagcaa
     4981 caacaaaatc agtgatattc ctgaatttgt tcgcggtatg gtaatggtta aaaaagccgc
     5041 agctatggca aacaaagagc tgcaaaccat tcctaaaagt gtagcgaatg ccatcattgc
     5101 cgcatgtgat gaagtcctga acaacggaaa atgcatggat cagttcccgg tagacgtcta
     5161 ccagggcggc gcaggtactt ccgtaaacat gaacaccaac gaagtgctgg ccaatatcgg
     5221 tctggaactg atgggtcacc agaaaggtga atatcagtac ctgaacccga acgaccatgt
     5281 taacaaatgt cagtccacta acgacgccta cccgaccggt ttccgtatcg cagtttactc
     5341 ttccctgatt aagctggtag atgcgattaa ccaactgcgt gaaggctttg aacgtaaagc
     5401 tgtcgaattc caggacatcc tgaaaatggg tcgtacccag ctgcaggacg cagtaccgat
     5461 gaccctcggt caggaattcc gcgctttcag catcctgctg aaagaagaag tgaaaaatat
     5521 ccaacgtacc gctgaactgc tgctggaagt taaccttggc gcaacagcaa tcggtactgg
     5581 tctgaacacg ccgaaagagt actctccgct ggcagtgaaa aaactggctg aagtcactgg
     5641 cttcccatgc gtaccggctg aagacctgat cgaagcgacc tctgactgcg gcgcttatgt
     5701 tatggttcac ggcgcgctga aacgcctggc tgtgaagatg tccaaaatct gtaacgacct
     5761 gcgcttgctc tcttctggtc cacgtgccgg cctgaacgag atcaacctgc cggaactgca
     5821 ggcgggctct tccatcatgc cagctaaagt aaacccggtt gttccggaag tggttaacca
     5881 ggtatgcttc aaagtcatcg gtaacgacac cactgttacc atggcagcag aagcaggtca
     5941 gctgcagttg aacgttatgg agccggtcat tggccaggcc atgttcgaat ccgttcacat
     6001 tctgaccaac gcttgctaca acctgctgga aaaatgcatt aacggcatca ctgctaacaa
     6061 agaagtgtgc gaaggttacg tttacaactc tatcggtatc gttacttacc tgaacccgtt
     6121 catcggtcac cacaacggtg acatcgtggg taaaatctgt gccgaaaccg gtaagagtgt
     6181 acgtgaagtc gttctggaac gcggtctgtt gactgaagcg gaacttgacg atattttctc
     6241 cgtacagaat ctgatgcacc cggcttacaa agcaaaacgc tatactgatg aaagcgaaca
     6301 gtaatcgtac agggtagtac aaataaagaa ggcacgtcag atgacgtgcc ttttttcttg
     6361 tgagcagtaa cttaaaaata acaacctaat atcaacttgt taaaaaacaa ggaaggctaa
     6421 tatgctagtt gtagaactca tcatagtttt gctggcgatc ttcttgggcg ccagattggg
     6481 gggaataggt attggttttg caggcggatt gggggtgctg gttcttgccg ctattggcgt
     6541 taaacccggt aacatcccgt tcgatgtcat ttccattatc atggcggtta tcgccgctat
     6601 ttctgccatg caggttgctg gcggtctgga ctatctggtt catcagacag aaaagctgct
     6661 gcgccgtaac ccgaaataca tcacgatcct cgcaccgatc gtgacctatt tcctgactat
     6721 ctttgctggt actggcaaca tatctctggc gacactgcca gttatcgctg aagttgcgaa
     6781 ggaacaaggc gtcaaacctt gccgtccgct gtctactgca gtggtatccg cgcagattgc
     6841 gatcaccgca tcgccaatct cagcggcagt ggtttacatg tcttccgtga tggaaggtca
     6901 tggcatcagc tacctccatc tgctctccgt ggtcatcccg tccaccctgc tggcggttct
     6961 ggtgatgtcc tttctggtca ctatgctgtt caactccaaa ctctctgacg atccgattta
     7021 tcgcaagcgt ctggaagagg gcctggttga actgcgcggt gaaaagcaga ttgaaatcaa
     7081 atccggtgca aaaacgtccg tctggctgtt cctgctgggc gtagttggcg tggttatcta
     7141 tgcaatcatc aacagcccaa gcatgggtct ggttgaaaaa ccgctgatga acaccaccaa
     7201 cgcaatcctg atcatcatgc tcagcgttgc aactctgacc accgttatct gtaaagtcga
     7261 taccgacaac atcctcaact ccagcacctt caaagcaggt atgagcgcct gtatttgtat
     7321 cctgggtgtt gcgtggctgg gcgatacttt cgtttccaac aacatcgact ggatcaaaga
     7381 taccgctggt gaagtgattc agggtcatcc gtggctgctg gccgtcatct tcttctttgc
     7441 ttctgctctg ctgtactctc aggctgcaac cgcaaaagca ctaatgccga tggctctggc
     7501 actgaacgtt tcaccgctga ccgctgttgc ttctttcgct gcggtgtctg gtctgttcat
     7561 tctgccgacc tacccgacgc tggttgctgc ggtacagatg gatgacacgg gtactacccg
     7621 tatcggtaaa ttcgtcttca accatccgtt cttcatcccg ggtactctgg gtgttgccct
     7681 ggccgtttgc ttcggcttcg tgctgggtag cttcatgctg taatgatcaa tcgcggggcg
     7741 ttcacgcccc gctttctttc ccgccgacta acatccttcc cccgtacgtt gtatagtgac
     7801 ctctctcttg cggttccatc tgttcttgcg aggtgtttat gcttgatgaa aaaagttcga
     7861 ataccgcgtc tgtcgtggtg ctatgtaccg caccagatga agcgacagcc caggatttag
     7921 ctgccaaagt gctggcggaa aaactggcgg cctgcgcgac cttgatcccc ggcgctacct
     7981 ctctctatta ctgggaaggt aagctggagc aagaatacga agtgcagatg attttaaaaa
     8041 ctaccgtatc tcaccagcag gcactgctgg aatgcctgaa gtctcatcat ccatatcaaa
     8101 ccccggaact tctggtttta cctgttacac acggagacac agattacctc tcatggctca
     8161 acgcatcttt acgctgatcc tgctactttg cagcacttcc gtttttgccg gattattcga
     8221 cgcgccggga cgttcacatt ttgtccccgc ggatcaagcc tttgcttttg attttcagca
     8281 aaaccaacat gacctgaatc tgacctggca gatcaaagac ggttactacc tctaccgtaa
     8341 acagatccgc attacgccgg aacacgcgaa aattgccgac gtgcagctgc cgcaaggcgt
     8401 ctggcatgaa gatgagtttt acggcaaaag cgagatttac cgcgatcggc tgacgcttcc
     8461 cgtaaccatc aaccaggcga gtgcgggagc gacgttaact gtcacctacc agggctgtgc
     8521 tgatgccggt ttctgttatc cgccagaaac caaaaccgtt ccgttaagcg aagtggtcgc
     8581 caacaacgca gcgtcacagc ctgtgtctgt ttcgcagcaa gagcagcaca ccgcgcaatt
     8641 gcccttttcc gcgctctggg cgttgttgat cggtattggt atcgccttta cgccatgcgt
     8701 gctgccaatg tacccactga tttctggcat cgtgctgggt ggtaaacagc ggctctccac
     8761 tgccagagca ttgttgctga cctttattta tgtgcagggg atggcactga cttacacggc
     8821 gctgggtctg gtagttgccg ccgcagggtt acagttccag gcggcgctac agcacccata
     8881 cgtgctcatt ggcctcgcca tcgtctttac cttgctggcg atgtcaatgt ttggcttgtt
     8941 tactctacaa ctcccctctt cgctgcaaac acgtctcacg ttgatgagca atcgccaaca
     9001 gggcggctca cctggcggtg tgtttgttat gggggcgatt gccggactga tctgttcacc
     9061 atgcaccacc gcaccgctta gcgcgattct gctgtatatc gcccaaagcg ggaacatgtg
     9121 gctgggcggc ggcacgcttt atctctatgc gttgggcatg ggcctgccgc tgatgctaat
     9181 taccgtcttt ggtaaccgct tgctgccgaa aagcggcccg tggatggaac aagtcaaaac
     9241 cgcgtttggt tttgtgatcc tcgcactgcc ggtcttcctg ctggagcgag tgattggtga
     9301 tgtatgggga ttacgcttgt ggtcggcgct gggtgtcgca ttctttggct gggcctttat
     9361 caccagccta caggctaaac gcggctggat gcgcgtggtg caaataatcc tgctggcagc
     9421 ggcattggtt agcgtgcgcc cacttcagga ttgggcattt ggtgcgacgc ataccgcgca
     9481 aactcagacg catctcaact ttacacaaat caaaacggta gatgagttaa atcaggcgct
     9541 cgttgaagcc aaaggcaaac cggtgatgtt agatctttat gccgactggt gcgtcgcctg
     9601 taaagagttt gagaaataca ccttcagcga cccgcaggtg caaaaagcgt tagcagacac
     9661 ggtcttactt caggccaacg tcacggccaa cgacgcacaa gatgtggcgc tgttaaagca
     9721 tcttaatgtc cttggcctac cgacaattct cttttttgac ggacaaggcc aggagcatcc
     9781 acaagcacgc gtcacgggct ttatggatgc tgaaaccttc agcgcacatt tgcgcgatcg
     9841 ccaaccgtga acgacacttg cagtgggaaa gacggaggaa aatagcgtgc aacgtgaaga
     9901 tgtactggga gaagccctga aattattaga attacaaggg attgccaaca ccacgctgga
     9961 gatggttgct gaacgtgtgg attatccact ggacgagcta cgccgcttct ggccagacaa
    10021 agaggcaatc ctctacgatg cgctgcgtta tcttagccaa cagatagatg tctggcgtcg
    10081 tcagctgatg ctggacgaaa cgcaaaccgc cgaacaaaag ctgctggcac gttatcaggc
    10141 gttatcggag tgcgttaaaa acaaccgcta tccgggctgt ctatttatcg ctgcctgtac
    10201 gttttatccc gatcctggcc accctattca tcaactggcc gatcagcaaa aaagcgcggc
    10261 ctacgatttc acccacgaac tgttaaccac actggaagtt gacgatccgg cgatggtagc
    10321 aaagcagatg gaactggtgc tggaaggctg tttaagccga atgctggtga atcgtagcca
    10381 ggcggatgtc gacactgcac atcggctggc ggaagatatc ctgcgcttcg cccgctgccg
    10441 ccagggtggt gcattgacct gacagaaaca cagaaaagaa gcgatttgcc gcaatcttaa
    10501 gcagttgaat cgcttttact gaaattaggt tgacgagatg tgcagattac ggtttaatgc
    10561 gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatccc cgtgtccttg
    10621 gttcgattcc gagtccgggc accaaattca tataaacgga cctccacgga ggtccgtttt
    10681 tcgtttcagg acgccacgat ttaagcgttc tgcctccaaa tcaattctcc cgaactcaac
    10741 cagattctcc cctcatcacc agcaatttgc gggcatatcc caattcggga aaatttgttc
    10801 ctgagcgata gcactaactg acgtgaaatg tcgtgcggcc ccgtgatgtt gttgaacgtc
    10861 aaatgacgtc atcaggagcg taacgcatcc ataaagcaca acaccggtct gaacgccgac
    10921 tgatgagatt ttctgaatga gaacaaagag aaatgtatca gtccgcttgc tcatgcaaag
    10981 actaacaatc cattaaaata gtaagcgctc cggacaattt tccatggatt attttctgaa
    11041 catttttctt tggcaaagat gatgaatttt gatggtaagg aatattactt ctgg
//
LOCUS       DAZFRN010000157        11047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFRN010000157 DAZFRN010000000
VERSION     DAZFRN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.159"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..414
                     /gene="ecpR"
                     /locus_tag="ACHKUB_004091"
                     /gene_synonym="matA"
     CDS             <1..414
                     /gene="ecpR"
                     /locus_tag="ACHKUB_004091"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO6657044.1"
                     /translation="DFVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALAN
                     YWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQ
                     PKSIARIENCSVKTVYTHRRNAEAKLYSKIYKLVQ"
     gene            489..1076
                     /gene="ecpA"
                     /locus_tag="ACHKUB_004092"
                     /gene_synonym="matB"
     CDS             489..1076
                     /gene="ecpA"
                     /locus_tag="ACHKUB_004092"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO6657045.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1134..1802
                     /gene="ecpB"
                     /locus_tag="ACHKUB_004093"
                     /gene_synonym="yagY"
     CDS             1134..1802
                     /gene="ecpB"
                     /locus_tag="ACHKUB_004093"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO6657046.1"
                     /translation="MKKHLLPLALLFSGIPPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1828..4353
                     /gene="ecpC"
                     /locus_tag="ACHKUB_004094"
                     /gene_synonym="yagX"
     CDS             1828..4353
                     /gene="ecpC"
                     /locus_tag="ACHKUB_004094"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO6657047.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4343..5986
                     /gene="ecpD"
                     /locus_tag="ACHKUB_004095"
     CDS             4343..5986
                     /gene="ecpD"
                     /locus_tag="ACHKUB_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO6657048.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5955..6665
                     /gene="ecpE"
                     /locus_tag="ACHKUB_004096"
     CDS             5955..6665
                     /gene="ecpE"
                     /locus_tag="ACHKUB_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO6657049.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6978..7307
                     /locus_tag="ACHKUB_004097"
     CDS             6978..7307
                     /locus_tag="ACHKUB_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6657050.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7555..8169)
                     /locus_tag="ACHKUB_004098"
     CDS             complement(7555..8169)
                     /locus_tag="ACHKUB_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO6657051.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8587..9276
                     /gene="paoA"
                     /locus_tag="ACHKUB_004099"
     CDS             8587..9276
                     /gene="paoA"
                     /locus_tag="ACHKUB_004099"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO6657052.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9273..10229
                     /gene="paoB"
                     /locus_tag="ACHKUB_004100"
     CDS             9273..10229
                     /gene="paoB"
                     /locus_tag="ACHKUB_004100"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO6657053.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10226..>11047
                     /gene="paoC"
                     /locus_tag="ACHKUB_004101"
     CDS             10226..>11047
                     /gene="paoC"
                     /locus_tag="ACHKUB_004101"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO6657054.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPS"
BASE COUNT         2814 a         2940 c         2876 g         2417 t
ORIGIN      
        1 gatttcgtgt ttatcaatct caatacggat tctttatctg aatttataaa ccgtgataat
       61 gaatggttat ccgcggtaaa gggaaaacag gtcgtattga ttgcggccag aaagtcagaa
      121 gccttagcaa attattggta ttacaatagc aatattaggg gcgtggtata cgctggactg
      181 agtcgtgata ttagaaaaga actggcctat gtgattaatg gcaggttcct gagaaaagat
      241 attaagaaag ataaaatcac ggaccgggaa atggaaatta tccgcatgac ggcccaggga
      301 atgcaaccta aatcgattgc cagaattgaa aattgtagtg tgaagacagt gtatacccat
      361 cggcgtaatg ctgaggccaa gctgtactca aaaatatata agttggttca gtaaactcca
      421 ggcaagttag ttttaaaaaa tgactcactg ggacatcacg tcctcaattc aactcgggaa
      481 gaaatgcaat gaaaaaaaag gttctggcaa tagctctggt aacggtgttt accggcatgg
      541 gtgtggcgca ggctgctgac gtaacagctc aggctgtagc gacctggtcg gcaacagcca
      601 aaaaagacac caccagtaag ctggttgtga cgccactcgg tagcctggcg ttccagtatg
      661 ccgaaggcat taaaggtttt aactcacaga aaggtctatt tgacgtggct atcgagggtg
      721 actcaacggc taccgccttt aaactgacct cacgtcttat caccaacaca ttaacccagt
      781 tggatacctc aggttccaca ctgaatgtgg gcgtggatta taacggcgcg gcagtcgaaa
      841 aaactggcga taccgtgatg atcgataccg ccaacggcgt actgggcggc aaccttagcc
      901 cgctggctaa cggttacaat gccagcaatc gtaccaccgc acaggatggt ttcaccttca
      961 ccatcatcag cggcaccacc aatggtacca ccgcagtaac cgattacagc actctaccgg
     1021 aaggcatctg gagcggcgac gttagcgtac agttcgacgc gacctggacc agttaatctc
     1081 tctgatgtac cagcaggggt agcccccctg ctttattcct ggacggaatt gttatgaaaa
     1141 agcaccttct gcctctcgct ctgctgtttt ccggaatacc tccggcccag gcgctggatg
     1201 tcggcgatat atcatcgttt atgaacagtg acagcagcac gctgagcaaa acgatcaaaa
     1261 acagtaccga cagtggtcgc cttatcaata tccgtctcga acggctctct tcaccgcttg
     1321 acgacgggca ggttatctca atggacaagc cggatgagtt gctactcact cccgccagct
     1381 tgctgctacc cgcccaagcc agcgaagtga tccgcttctt ctataaggga cccgcagatg
     1441 aaaaagagcg ctactaccgc attgtctggt ttgatcaggc cctcagtgat gcgcagcgcg
     1501 ataatgccaa ccgcagcgct gtggccactg cttccgcccg catcggcacc attctggtcg
     1561 tcgcccctcg tcaggcgaac taccactttc agtacgccaa cggctccctg acaaatacag
     1621 gaaatgcgac gctgcggatc ctcgcttacg gcccctgcct gaaagccgcc aatggtaagg
     1681 agtgtaaaga gaattactac ctgatgccag gcaagtcgcg tcgttttacc cgcgtggaca
     1741 cggcggataa caaaggacgg gttgcacttt ggcagggtga taagttcatt cccgtgaaat
     1801 agatagctgt gcagatggat aacgacaatg cctttacgac ggttctcccc aggactgaaa
     1861 gcccagtttg ccttcggcat ggtctttttg ttcgttcaac ccgatgccag cgctgctgac
     1921 ataagtgcgc agcaaatagg tggggtgatt attccgcagg ccttcagtca ggcgcttcag
     1981 gacggcatga gcgtcccgct ctatattcat ctcgccggta gccagggtcg ccaggacgat
     2041 cagcgaatcg gcagcgcttt tatctggctg gatgatggac agctacgcat ccggaaaata
     2101 cagctggaag agagtgaaga taacgccagt gtcagcgaac aaactcgaca gcagctgatg
     2161 gctctggcca acgccccgtt caatgaggcc cttaccatcc ccctgactga caacgcgcag
     2221 ctggatctca gcttgcgcca actgctgctg cagctggtgg tcaagcgcga agcgctgggc
     2281 accgtactac gctcacgtag cgaagacatc gggcagtcca gtgttaacac cctcagcagt
     2341 aatctgagct ataacttggg cgtctataac aaccagttgc gtaacggcgg gagcaacaca
     2401 tccagctatc tgtcgctgaa taacgttact gcgctgcgcg aacatcatgt ggtgctcgac
     2461 ggctcgctgt acgggatcgg tagcggtcaa caggacagtg aattatataa agcgatgtat
     2521 gaacgcgatt ttgccggtca ccgatttgcc ggtggaatgc tcgacacctg gaacttgcag
     2581 tccttagggc cgatgaccgc catttcagca gggaagattt acggcctttc ctggggaaac
     2641 caggccagct ccaccatctt cgacagcagc cagtcagcca cgccagtgat cgccttttta
     2701 ccggcggcgg gcgaagtaca tctcacccgt gatgggcggc tactaagcgt tcagaacttc
     2761 actatgggca atcatgaagt ggatacccgg ggtctaccgt acgggattta cgatgtggaa
     2821 gttgaggtga tcgttaacgg tcgcgtgatc agcaaacgcc cccagcgggt caataagctg
     2881 tttagccggg ggcgcggcgt cggtgcacca ctggcgtggc aggtatgggg cggtagcttt
     2941 catatggatc gctggtcgga aaacgggaaa aagacgcgac cagctaaaga gagttggcta
     3001 gcaggtgcct cgacctccgg ctcattgagt acgcttagct gggcggcaac gggatatgga
     3061 tacgataatc aggcggtggg tgaaacccgt ctgacgctgc cgcttggggg ggcgatcaac
     3121 gttaacctgc aaaatatgct ggccagtgac agctcatgga gcagcatcgg cagcatcagc
     3181 gccactctac cgggaggctt tagttcgctg tgggttaacc aggaaaaaac ccgcattggc
     3241 aatcaattgc gacgtagcga tgccgacaac cgtgcaatcg gcggcacact caacctgaac
     3301 tcactgtggt cgaagctggg cacattcagc atcagctaca atgatgaccg ccgttacaac
     3361 agccattatt acacggcaga ttactatcaa aatgtctaca gcggtccctt tggttcgctt
     3421 ggcctgcggg ccggtattca gcgctataac aacggcgaca gcaacgccaa tacagggaaa
     3481 tatatcgctc tcgatctctc gctaccactg ggcaactggt ttagcgcagg gatgacccat
     3541 caaaacggct acaccatggc aaacctgtca gcacgcaaac agtttgatga aggaaccatt
     3601 cgcactgttg gtgccaatct gtcacgtgcc atctccggcg ataccggtga tgacaaaacc
     3661 ctcagcggtg gggcgtatgc acagttcgac gctcgttacg ccagcggaac gctgaacgtc
     3721 aatagcgcgg cggacggcta catcaatacc aacttgaccg ccaatggcag cgtcggctgg
     3781 cagggtaaaa acatcgctgc cagcgggcgg actgatggca acgctggggt gatattcaac
     3841 accgggctgg aggacgacgg tcagatcagc gccaaaatca acgggcggat tttcccgctt
     3901 aacggcaagc gtaactatct cccgctctct ccctatggaa gatatgaggt ggagttacag
     3961 aacagcaaaa actcactcga cagctacgat atcgtcagcg gccgcaaaag ccatctgact
     4021 ctctatccag gcaatgtcgc tgtcattgag ccagaggtga agcagatggt taccgtctcc
     4081 ggtcgtatcc gtgcggaaga cggcacactg ctggctaacg cacggattaa caaccatatc
     4141 ggccgaaccc gaaccgatga aaacggcgag tttgtcatgg acgtggataa gaaatatccc
     4201 actatcgatt ttcgctacag tggcaataaa acctgcgaag tggcactgga actcaaccag
     4261 gcgcgcggtg ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc gtgggcggcg
     4321 gtgacgcaga caggagaaga gaatgagagt taacctacta ataacgatga taattttcgc
     4381 gctaatctgg ccagtaactg agctcagagc ggcagtgagc aaaacaacct gggcggatgc
     4441 accggcacgc gagtttgtgt ttgtcgaaaa caactcagac gacaactttt tcgtcactcc
     4501 tggcggggcg ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt taaaatacac
     4561 tggttcagga accatctatc agcaaagcct cggctacatt gataacggtt acaacaccgg
     4621 cctttatacc aactggaagt ttgatatgtg gctggaaaat tcaccagttt catctccttt
     4681 aactggcttg cgctgcatca actggtacgc tgggtgtaat atgaccacca gtcttatcct
     4741 gccgcaaacc accgacgcca gtggatttta tggcgcgacg gtgaccagcg gcggcgcgaa
     4801 gtggatgcac ggcatgttgt cagacgcgtt ttaccagtat ctgcaacaaa tgcccgtcgg
     4861 cagcagcttt acaatgacca tcaatgcctg ccagacctct gtgaactatg acgccagcag
     4921 cggcgcacgc tgtaaggatc aggcctccgg caactggtat gttcgcaacg tcacccatac
     4981 gaaagcagca aatctgcggt taataaatac ccactcgctg gcggaggtat ttatcaacag
     5041 cgacggagta ccgactctgg gcgaagggaa cgccgactgc cggacacaga ccatcggcag
     5101 cctttcagga ttaagttgta agatggtcaa ctataccctg caaacaaacg gactcagcaa
     5161 cacctcaatc catatattcc cggcgatcgc caactcgtcg ttagcctcgg ccgtcggggc
     5221 gtacgatatg cagttcagtc tgaatggcag ttcatggaaa ccggtgagca ataccgccta
     5281 ttactacacc ttcaacgaga tgaagagcgc agactcgatc tatgttttct tctcgagcaa
     5341 cttctttaag cagatggtga acctcgggat cagcgatatc aacaccaaag atctattcaa
     5401 ctttcgcttt cagaacacca catcaccgga gtctggctgg tatgaatttt ctacctccaa
     5461 cacgctgatt atcaaacccc gtgatttcag catcagtatt atctccgatg aatatactca
     5521 gacaccgtcg cgggagggat atgttggcag cggcgagtcg gcactcgatt tcggctatat
     5581 cgtaaccacc agcggtaaaa cagctgccga cgaagtgctg atcaaggtga ccggacccgc
     5641 gcaggtgatt ggcgggcgct cctattgtgt cttcagctcc gatgacggta aggcgaaagt
     5701 accgttcccg gcgacacttt cctttattac ccgcaacgga gctacaaaaa cctacgatgc
     5761 cgggtgcgat gatagctggc gggatatgac cgatgcgctg tggttgacca caccgtggac
     5821 tgatatctct ggcgaagtgg ggcagatgga taagaccaca gtcaaatttt cgattccaat
     5881 ggataacgcc atttcgctgc gtacggtaga tgataacggc tggtttggcg aagtcagcgc
     5941 ttcaggagag attcatgttc aggcgacgtg gcgtaacatt aactaaggcc ctgctgacag
     6001 cggtctgtat gctggcggca cctttgacac aggcgatttc ggtcggcaat ctgacatttt
     6061 cgctgccgtc cgagactgac tttgtcagca aacgtgtagt gaataacaac aaaagcgcac
     6121 ggatataccg tattgccatc agtgctattg atagcccggg cagcagtgaa ttgcgcaccc
     6181 gaccggtgga tggtgaactg cttttcgccc cccgccaact ggcgttgcag gctggtgaga
     6241 gcgagtattt taaattttac tatcatggtc cacgggataa ccgcgagcgc tactaccggg
     6301 tctcatttcg cgaagtcccc actcgtaacc agacaagacg tagcccaacc ggcggcgagg
     6361 tcagcacgga gccggtggtg gtgatggata ccattctggt agtacgacca cgtcaggttc
     6421 agtttaaatg gtccttcgac aaggtgacag gaacggtaag taacactggc aacacatggt
     6481 ttaagctact gattaagcca ggatgtgatt cgaccgaaga ggaaggcgat gcctggtatc
     6541 tacgtccggg agacgtggtt catcagcctg agttacgtca gccggggaat cattatctgg
     6601 tctataacga caaattcatt aagattagcg attcctgtcc ggctaagccc ccttcggcgg
     6661 actaagactt atccatcggt cgagggaaga attccacctc agagccccaa aaatcgttta
     6721 aatgatgtgg aagcgatcgt caatatcacc tgtaacgatg ggctttctca tatgcacacc
     6781 atagtgttca ggagacacgg aaaggtatca ggcagcctgc taacgagtgg tttaatcacg
     6841 aattagtacg taaaatcggt aacggctgga aatcattcaa tactcacact atcgaaagtt
     6901 ccccagccaa ccgcggtacg ttcttacata cgatgtaccg ctgttctctt tacgattttt
     6961 agctgtactg gtgaattatg agcaatctga atccatgcat gacgtgtggt gcctgttgtg
     7021 catttttccg cgtctctttt tactgggccg aagccgacga tgctggcggt actattcccg
     7081 ccaggctcac tgaacaaata tccccttttc accgatgcat gagcggtacc aatcagaaaa
     7141 acccccgatg tattgccctt gcaggaaccc cgggcaaaaa tgcctgctgc acgatatata
     7201 aaaatcgatc gtccacatgc agagaattcg ccatgtctgg tgagaacgga gtcgtcaatg
     7261 aggcttgcaa tcgtgcaagg gctaaatacg ggctgacacc gttataaaca tacaataatt
     7321 aattgcaatg ccccgccagc gataatagcg gggcttcgtt tttcaggggt aacaaaaccc
     7381 gatacttctt ctatttgcca gcaacaatgc ctctcttctg tagcgttctt cggatctaca
     7441 tcatcctgaa gtagcgcgat atactcactg actatctgca tcagaatata aaaagcaatg
     7501 tttttaacct ataaaaatgg cgctgtattt gcgccatttt tatcattcaa tgcattatct
     7561 gtttgagcct aaagggatct cagggtctgg ctcatgagta attctgtttc gtaaatctct
     7621 gcgaataatt tcaatagacc agaaccagac taaatgtcca aaaatttcag aaacattctc
     7681 ataccacggg agatcaaaca gaggtggcgt cagtcccatg agagggaatg aaatcatatg
     7741 aacaaaaagt tgggctaaag cacctgccag taagccctgc cagagtttaa tttttggaaa
     7801 tacttcagcg accacacaat aaccgacagc aaacactatc gaaaagataa tgtgcgtaac
     7861 accaacccag ttaaagacat gcccggcaaa ggtataaaca gccgcattgg gatctgtcag
     7921 ccccaaccag tctcgaagaa aaatatacgg tggattgaga aaattacgcg agcaatcaat
     7981 ttggcctgca gccctgatta atgattccgg gccacacgct gcattaaaca tatccaccgg
     8041 gctacgtggc ggcaatggaa cttcagcccc ccacttcacg aatgcggaaa caacgccagc
     8101 aatcagccca atgaatgcag caagaccata acgtctgcgg ttcggtggag tttgttcaaa
     8161 tatattcata tctaccctgc ttgtaccatt atgttataca cctcttcagg agtattcata
     8221 aaacaaggca aatgtaaaga actgtattgt tttgtataac aagatggttt cctaatcgcc
     8281 aatgaatata agctccatca tttctcccta tttttatatt agaagtgaca gagatttgca
     8341 gggtgatgca gagctgaaaa tcacaggttt ccttattggt ttttgcatcg tacaactaaa
     8401 gcaataaacc agctcccatc atcatcaaca tcacggcgaa catgaattta ttagccgggc
     8461 tcatagctgc attcgcgttt taagaataat cctcctgctg tcgccgacta tgcttaacat
     8521 ttaaaaaagc atcagcactc tcgcaacgca ctcttatttt cccctttaga ataccggagg
     8581 cctggtatga gcaaccaagg cgaatacccc gaagataatc gggttgggaa gcacgagccg
     8641 cacgatttaa gtttgacccg tcgcgatctg attaaagtga gcgccgcaac agcggcgacc
     8701 gccgtggttt atcctcattc tacgctggcg gcaagcgttc cggcagctac acccgcgcca
     8761 gagataatgc ccctgacact gaaggtgaac ggcaaaaccg agcagcttga ggtggatacc
     8821 cgaaccacgc tactggacgc tttgcgtgaa aatctgcatt tgatcggtac caagaaaggt
     8881 tgcgatcacg gacagtgcgg agcctgtacc gtgctggtca atggtcgcag gcttaatgcc
     8941 tgcctgacgc ttgcagtcat gcatcagggg gccgagatca ccaccattga aggcctgggt
     9001 tcgccagata atcttcaccc catgcaggcg gcctttatca agcatgatgg cttccagtgc
     9061 ggctactgca cctccgggca aatttgctca tcagtagcgg tgctaaaaga gattcaggac
     9121 ggcattccca gtcacgtcac ggtcgatttg gtttccgctc cagaaacaac tgccgatgag
     9181 atccgtgaac gtatgagcgg caacatctgt cgctgtggtg catacgctaa catccttgcc
     9241 gccattgaag atgctgcggg ggagataaaa tcatgaaggc gtttacctat gaacgagtga
     9301 acaccccagc cgaggcggca cttagcgctc agcgcgtacc cggcgcaaaa tttatcgcgg
     9361 gcgggaccaa tctgctggac ctgatgaagc tggaaattga aacgcccacc caccttatcg
     9421 atgtgaacgg cctcgggctc gataagattg aagtgaccga cgcgggtggg ctgcgcatcg
     9481 gcgcactggt acggaacacc gacctggcgg ctcacgagcg cgtgcgtcgt gattacgcgg
     9541 tactctcccg cgccctgctc gctggcgcat ctggtcagtt acgtaatcag gcaaccaccg
     9601 caggtaatct gctccagcgc acgcgctgcc cctattttta cgacaccaat cagccctgca
     9661 ataagcgcct gcccgggagc ggctgcgcgg cgcttgaagg ctttagccgt cagcacgcgg
     9721 tggtaggcgt aagtgaagcc tgcatagcca cccatccgag cgatatggcg gtcgcaatgc
     9781 ggttgctgga tgcggtggtg gaaaccatca cgccggaggg aaagactcgc agtatcacac
     9841 tggctgattt ttatcaccca ccggggaaaa cgccgcacat tgaaaccgcc ctgcttcccg
     9901 gtgagcttat cgttgcggtg acgttacctc caccgctcgg cggaaaacat atctaccgta
     9961 aggtgcgcga tcgcgcctcc tacgcctttg ccctggtatc ggtcgcggcg attattcagc
    10021 ctgacggcag cgggcgcgtc gcgctgggcg gagtagcaca taagccctgg cgcattgagg
    10081 ctgcggatgc tcagctatcc cagggggcgc aggccgtata tgacacgctg ttcgccagcg
    10141 cccatcccac cgctgaaaac acctttaaac tcctgttggc gaagcgaacg cttgcctccg
    10201 tactggctga agcgagggca caggcatgaa atttgataaa cccgcagggg aaaacccgat
    10261 cgatcagctg aaggttgtcg gtcgtcccca tgaccgcatc gacggaccgc tgaaaactac
    10321 cggcacggca cgctacgcct acgaatggca tgaagaggcc cccaacgccg cctatggcta
    10381 tatcgtcggt tccgccattg ccaaaggacg cctcaccgcc cttgatacgg acgccgcgca
    10441 aaaagcgccg ggcgtactgg ctgtcattac cgccagtaac gccggggcac tcggcaaagg
    10501 cgacaaaaac accgccagac tgttaggcgg ccccactatt gagcactatc atcaggccat
    10561 tgcgctggta gtggccgaga ccttcgaaca ggcgcgagcg gcggcctcgc tggtgcaggc
    10621 acactatcgc cgtaataaag gagcttactc cctggcggac gaaaaacagg ccgtcaatca
    10681 gccgccggaa gacacgcccg acaaaaacgt cggtgacttt gacggggctt tcacctccgc
    10741 tgcggtgaag attgatgcta cctacacgac cccggaccag agccatatgg cgatggagcc
    10801 gcatgcctcg atggccgtct gggatggaaa taagcttacc ctctggacct caaatcagat
    10861 gattgactgg tgccgcaccg atctggcaaa aacgctgaaa gttcccgtgg agaatgtgcg
    10921 tattatctcc ccgtatatcg gcggagggtt tggcggcaag ctgttcctga gaagcgatgc
    10981 gctgctggcg gccctcgccg cccgagcggt gaaacgtccg gttaaagtga tgctcccccg
    11041 cccctct
//
LOCUS       DAZFRN010000158        10892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFRN010000158 DAZFRN010000000
VERSION     DAZFRN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.160"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..940
                     /locus_tag="ACHKUB_004102"
     CDS             <1..940
                     /locus_tag="ACHKUB_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO6657055.1"
                     /translation="GDAMAIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLT
                     LVTMTMQDHATLKEIKDLAKNITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQV
                     VGLTMKCYSPLCKNASRMDQNLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEV
                     REEMRSLGSYIALNLEGSSQERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARV
                     LVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTR
                     WPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
     gene            complement(1202..9184)
                     /locus_tag="ACHKUB_004103"
     CDS             complement(1202..9184)
                     /locus_tag="ACHKUB_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFO6657056.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQFAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQVLTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDRPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTRGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVELQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLKALNTNENGIAEVTLKGTVLGVHTAEAILLNGKSDTKIVNIVPDTSNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLPVTFSTNPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVTFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNVFPVKGVTVNFTSRTNSAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLRVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTAS
                     MTGGKSEQLVVNFIADTLSAQVNLNVTEDNFIANNVGMTTLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTATLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTISST
                     SVETDDQGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVSMT
                     PERNGSYMVKASLANGASLEKQLEAIDEKLTLTASSPLIGVYAPTGTTLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTTVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGMEFVQSGTNVPYMKISAIDYSQ
                     NINGDYKATITGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNWER
                     ITATPKSGGPSYVYEIRVKSWWVNSGDAFMIYSLAENFCSSNGYTLPRADHLNHSRSR
                     GIGSLYSEWGDMGHYTTDAGFQSNMYWSSSPANSSEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(9498..9573)
                     /locus_tag="ACHKUB_004104"
     tRNA            complement(9498..9573)
                     /locus_tag="ACHKUB_004104"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9538..9540),aa:Asn,seq:gtt)
     gene            complement(9674..10471)
                     /gene="mtfA"
                     /locus_tag="ACHKUB_004105"
     CDS             complement(9674..10471)
                     /gene="mtfA"
                     /locus_tag="ACHKUB_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO6657057.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLRAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            10565..10654
                     /locus_tag="ACHKUB_004106"
     tRNA            10565..10654
                     /locus_tag="ACHKUB_004106"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10599..10601,aa:Ser,seq:cga)
BASE COUNT         2602 a         2606 c         2630 g         3054 t
ORIGIN      
        1 tggcgatgct atggccatct ttcctgttat tcgggcgctt gaattgcata aaattaagca
       61 tcttttaatt gttacgtcga caattaattt agaagtgttt aatgcactta aacttgaaaa
      121 gactaaatta acattagtca caatgactat gcaggatcac gcaacattaa aagaaataaa
      181 agatttagca aagaacataa cacagcaata cggtacgccg gatctttgta tagaggcgat
      241 gcgtaaaaag aacctgaaaa cgatgatatt tatcagtcag ttgaaagcaa aaacgaattt
      301 tcaggttgtt ggtttaacca tgaagtgcta ctcccctttg tgcaagaacg cgtcccggat
      361 ggatcagaat ctccgggctc ccgtacccat gacatgggca tttatgatgc gtgaggcggg
      421 ttttccagca gtcaggccaa tatatgaatt gccactaagt gaggatgtac tcgatgaggt
      481 gcgcgaggaa atgcgatcgt taggatctta cattgcgctt aatttagaag gtagctcgca
      541 ggaacgtaca ttttcattat cgattgcaga aaatctaata gcaaaaattc aaagtgaaac
      601 agatatacca atagtgatcg ttcatggacc aaaaggggaa gataaagcca gggtattagt
      661 ggattgttat aataatgtct accgtttatc cttatcaccc tcgattaaac gttcagcagc
      721 aatcataaaa gatgcttata tcgcaataac tcctgacacc tcaatattac atatggcaag
      781 tgcctataat actcccgttg ttgcaattta tgctgattac aaaacgcgat ggcccgcaat
      841 ggccgatgtt tcggagtcag tcgtcgttgg gcaaaaaatt gacaatataa gtctggatga
      901 attcgcaaag gcattaaaaa gtgttttggc gagaatatga gacagtactt atgcatagta
      961 atgccgatct gttatgaacg gtggtgtcat caatactctg gggctggtca atcatcacag
     1021 aaaaccagcc tttggatcaa agctgtactc accggatacg atcatagtca catcgaacgc
     1081 gattcttgcg gtgcagcagt tttatttatt cattttctgt gagagaaggg caagctctat
     1141 agagggaatg taaaaaagag tgctccgaat ggagcactct ttaagaaatt ataacggaaa
     1201 atcacaggtt tttataacat gtcgcataag caaacccaag cttttcaaat acgctttgat
     1261 cacctgttgc cagggaaact acgtattgtt cgcttgagtt tgcgggacta gatgaccaat
     1321 acatatttga ttgaaaacca gcgtcagtcg tgtaatgccc catatctccc cattcactgt
     1381 acagtgaccc gatgcctcgg gaacgactat ggtttaaatg atctgcacgg ggaagtgtgt
     1441 agccattgct actgcaaaaa ttttcagcaa ggctgtatat catgaaagca tcgccggagt
     1501 tcacccacca actcttcaca cggatttcgt atacatagct agggcctcct gattttggcg
     1561 tcgccgtaat cctttcccaa ttgctgccga catttttaaa tgtcacttta ccggtagcat
     1621 caacatcgac ccaggaggca gagcttgaaa actcataatc agccgccgtt tttcctgggg
     1681 caaagttgtc attattcaac tgataatacg ccccggtgaa cccctgcgaa gggaatgtag
     1741 ttgtcggtag gtcagtacca ttgactgata ctgtaccgct cattattttg tcttctgcgc
     1801 gagtgaattg tattgtggta ctcagaccag cttgatgaac accattcaat acagggatca
     1861 gcgttgcgat accctctccg ccgcctgtaa tggtggcttt gtaatcaccg ttgatatttt
     1921 gactgtaatc aattgcgcta attttcatat agggcacgtt agtacctgat tgcacaaatt
     1981 ccatcccttc agaaactttg atcggattgc ctgatatatc gtgcaaaaca agatgtagct
     2041 cagcactatc ggtaaagtcc cctttcagtg aggaccgatt gttcttaagg acggactgcg
     2101 aggcgtctgc cgggcctgcc accagcgtta tatctactgt ttgcattcca ccagcggtcg
     2161 tgactgcgct taccgtgacg ctacctgtca tcgctccttt cacgcttgtt gtcgcgattc
     2221 cgttttgatc ggtcaccgct gttaatgacg ttaatgtggc agagccgctt tttaaggcga
     2281 agtacacagt gagaccttcg atcaggttac cactgccatc ctcaaccgtt gcctttaagg
     2341 tacttaaatc actgttggtg gcggcgatag tcgatggatc agcgataaag ctagcaacat
     2401 gtgcggtgct tgagttgcca gtgactttca cggttgtctg tgtctgtatt gttacgccgt
     2461 tatggaacga tgcagtcacc gtatatgtac cgactttatt gctggtcaga acgaccggag
     2521 cctgacctga agagttagtt ctcacttttc cgccacttaa cgtcgcccct tctggcgtta
     2581 cgctaaagtt gatgacctga ccctccactg gagtgccatt tgcagaggtt agcgttgccg
     2641 tcagagtagt gcctgtaggg gcatagacac cgataagcgg actggacgcc gtgagtgtca
     2701 gtttttcatc aatagcctcc agttgtttct caagtgaggc tccattcgcc agggatgctt
     2761 tcaccatata cgaaccgttt ctttcgggcg tcatggagac ctcggctata ccatgcgtat
     2821 cagtagagac aatattttgg ctgatggtca tgtgctctgg tggctctgca ctgaatgtta
     2881 cagattcatt aagaatcggg ttgccaaact ggtcgttgac gtgagcttta actgctacgt
     2941 cttgtgcgac catgacctga ccttcaggta tctccagact ggtaatcgtt gcagaattaa
     3001 tatctgcttt tgcattcagt aatcgcgata tgacttcagt aggtttatct gccagagagg
     3061 ctgaaactgt tttcagtccg acttcggtgc ttgtcacaag aatttcagcg aaaccctgat
     3121 catccgtttc aacgctggtg ctgcttatcg tgacggaggt tccgcggaaa ttaactttta
     3181 tgccttctac cgggttgccg ttagcgtcag tgacgcttgc agtcatggtc gcgccctcgg
     3241 tcgtgctgac gacgaatgag tatacagagg ttaaggaggc tagtgttgcg gtaccagcat
     3301 cggcaatcaa agtcacctgt ttcgtatcac tgacaccata attgttcacg ctaactgtca
     3361 cggggtaggt accggatttt gtaccgctca gtgtaacttc agccttgccg ttgatatcag
     3421 taatggcgga accgccttgt ccgagagtga agcttgcgct cacgtctgcc ggtagcgtga
     3481 atgtcaccgc ctcattggct aacgggttgc cgtttccatc agtcactgtt gcctgaagtg
     3541 tggtcatccc aacgttattg gcgataaagt tgtcctcggt aacgttaaga ttaacctgcg
     3601 cactgagcgt atccgcaata aagttcacca ccaactgctc actcttaccg ccagtcatcg
     3661 atgctgtaac agtatgagcg cctgcttttg tacctttcag cgtgactttc gctttgcctt
     3721 cagcatcagt aatcgcttta ccgccatcgc tcagcgtgaa gttcgccttc acatcttccg
     3781 gcagagtaaa tgttacttca gtgttggcta tcgcattgcc ctctgtatca gcgactgttg
     3841 ctgttagtgt cgtgagatcg ttattgtcgg caatcaccgg atccttcgag gctgccagcg
     3901 taatttcggc attcgcgacg ttcggcacat aggtcactgt ttgttgcatc gaatcgccat
     3961 tttcgagggt tgccgtcact tgataaaccc cggcttttgt agcggtaaag cttgcgtaaa
     4021 aattgccgtc gtggttggta gtatatatag cgttattact gggggtcacg ccttcacttg
     4081 gtgatactct gagggttacc tcctgctggg gtacaccgtt gccgtaatta tccttcacct
     4141 caatatacag attggtagtg tcgcctgcgg agactgtatc aaaaagtgcc tgtagcaagg
     4201 tgagatgtgc cgtagacgca tcagcgttga cattaattga tgtgctaagc gtggagctac
     4261 cattttccag actggcctca acggtatccg gtctcgctcc tgattctatc gaggagcggg
     4321 tattggtata agtgacggta gccttaccct gttcgttcgt tacggcttgg ccgccattcg
     4381 tcatttcggc agagtttgtt ctgctggtga agttcacagt cacacctttc accggaaaga
     4441 cattattatc aacgactgtg gcggtgatga cgctgccgga gctgttctgc ggggtaccgg
     4501 cgattatgct gtctgggaca ggcgtcaact cgataatttt tgccgctgct gtgtcgccaa
     4561 taaaatgcac agttttgttg tcgctggcac cattattagc cagtgatgca gtgaccgtct
     4621 gctcaccaaa ggcaacgcct gcgagagtgg cctgcgcgat gccagactca ttggtattac
     4681 ttactcccgg ggtcagggtg agtcctgaag aggctgagct gaatgttacc ggaagattat
     4741 tcacctcatt gtcgaactga tctttaaccg ttgcagttag cgttgcgtta tcgacgccat
     4801 tacctgtgat ctcatctttt gacatctgca ggacaacctg agccgaggtt ttgtccgcca
     4861 caaacgttac cggctgcgaa tcactggtat tgttattacc cagcgttgcg gtaaccgtat
     4921 gcgtacccgc ttttttacct ttcagcgtga catgcgcttc cccattggct tgggtgatgg
     4981 caatgccgtt attttcgagg gtaaagtttg ccgcaacgtc ctgtggcatg gtgaaggtca
     5041 ccgttattcc cgccaccgga tgattcgacg gatctttcac cgtcgccgtc agctgcacgc
     5101 tgtcgctgtt attcgtcacc acctgttgag cagggatgtt cagggtgacc tgcgcgttgc
     5161 tggcatcggg cgcaatattg actgtcgtcg tatagccatt gccgttaagc agtgtggctt
     5221 cgactgtatg aacacccaaa accgtgccct taagggtaac ttcagcaaca ccactgtcgt
     5281 tagtattgct cgtgctctga ctaagttgag tatctgccgg gttggtactg aaggtgaccg
     5341 gtaggtcttt tactgcgtta tcaaaaggat ctttcactgt tgccgtcaga gtcgtctcat
     5401 ccacgccatt cccaatgatt tccgctttcg atgtttgcag aacgacaact gcgctgtcct
     5461 tatctgccac gaaggtgacc ggttgcgcat cgctggcatt attgttaccc agcgttgcgg
     5521 taaccgtatg cgtgcccgct tttttacctt tgagggtgac atgcgcttcg ccattggcct
     5581 gagtgatggc aataccgtta ttttcaaggg taaagtttgc cgccacgtcc tgaggcatgg
     5641 tgaagttcac cgttattccc gccaccggat gattcgacgg atctttcacc gtcgccgtca
     5701 gctgcacgct gtcgctgtta ttcgtcacca cctgttgagc cgggatgttc agggtgacct
     5761 gcgcgttgct ggtatcgggc acaatattga cgattttcgt atcgctttta ccgttaagca
     5821 gtatggcttc ggctgtatga acacccaaaa ccgtgccttt aagggtaact tcagcaatac
     5881 cgttctcatt agtattaagc gccttcagac tcagttgcgt gtctgcgggg gaggtgcgga
     5941 agactactga cagattttta accacgttat caaaaggatc tttcactgtt gctgtcagag
     6001 ttgtctcatc cacgccattc ccaatgattt ccgctttcga tgtttgcaga acgacaaccg
     6061 ctctgtcttt atccgccaca aacgtcattg gctgtgtatc gctgacattg ctgttagcca
     6121 gacgagccgt gatcatatgc gtgcccgcta acgtgccggt cagggaggcg attgcagtcc
     6181 cgttgctatc cgtcatgcct tttccgctgt tgctaatcga gaaccgactt gcgacgtcgt
     6241 ttggcacaga gaaggtgatt tctttgtctt ttaatgggtt gccattttta tcctgcaagg
     6301 ttgcagtcat atgtagcgga gctgtgttgg tgacggtgat atcacctgaa ggcacactga
     6361 gggtcagggc agcagtactt tgatcaccga taaaattcac ctgttgatta gcctgggaac
     6421 cagagctcac agaggccgta accctataat caccattttt caaactggtc agcgtagctg
     6481 tggcgatccc gtcgtggcta ttcacttctg tttggctcag tttcgcctct gctgaattaa
     6541 cattgaaagt gaccttgacg tcattgagca ggttgccttt tgcatcccgg actgtcgcgg
     6601 tcattgtggc gctatcattg ccgtcagcga ccacttcatt tttcgacttc tgcagctcaa
     6661 cctgcgcggt actcgagtcg ccgacaaaac tgacgattaa cgtttgtttc acgccatttt
     6721 caagggtgac ttcaaccgtg ttgtcttcct gcttactact tttcagatca aaagtcgcca
     6781 gaccattaac atccgttttt gcggtgtttt gattgttgaa ggaagttgcc gatccgctta
     6841 ataccgcaaa cgtgacggta tgatcattga ttgggttgct tccttcatca gcgacattga
     6901 ccgagacggt gtttgctgca ttctcattgg cgagcacacc attattgctg gcagataatg
     6961 tcgcaatttt cgctgactgc gggttggcgt cgatgataaa tccagcggta tgtaaatctt
     7021 cattccagtt ttgcattaat agcttcgcag taagcccact gcctctggta taggcggtat
     7081 aggtcgcctt atagacgcca tctgcggttt ctttccagtc tgtagtgact cctggtttca
     7141 cgttgtcgat gctgactgcg gtattcagtt gctgtttttg ctccttaaca ggtctgtcat
     7201 tttcatctct cagttctacc gtcacctcga taggattgcc ggagagataa cggtccttat
     7261 caatcttaat tgacgagtga gttcgggatg acgaaacaga aatgatattc accacggcgg
     7321 gggctttagc cgcatccaca ccgttcagct gtggcataag cgtcagcgtg ccagacatcg
     7381 ctcctgtggt caggacttgg gtatagcttc cgtcaccatt atctttccag tccgacaggg
     7441 tgatgtcctg aacaccttcg tgacgcgtcg aaagcaccag cccgataaca ggattacctg
     7501 ctgcatcatg cgcaataaaa gttagtgtgg cggttgaatg ggaatccgcg ttcaatgttt
     7561 gggtacttaa tgatactgag gaatctttct ggcttagcgt aggtgcctga acgaccacca
     7621 tgctctgttc acgattcgac aaattgcctt tgacatcttc agcggtgact tcaatcggcc
     7681 aggtgttatc ggtttctggc gtactggtga accggtacgc cggcagggta accagaatat
     7741 ctttacccgt tgtaaccact ttaccaccgg ccgcttccag agcggtggct tcgacgttat
     7801 agcctttcag ggcatatttg gtttgtagcg acgaaaccag tgatttcact tctcctgact
     7861 tccctgtcac ggggtctgtc agggtcaggc gaaccagttc ttttttgcga tattccagaa
     7921 cgatattgtt gttgcgatcc accagatcat aacggctgcc tgcaaggcta cgccgtgcag
     7981 cgacttcgtt cgggtcaagc tgtttctgca ttgcgctgcc aggttgccag gtaaaatcga
     8041 cggcaaaacg ggtgtcattt tcgccctgtt taccctggcg ttgctccgcg ctgaaggtca
     8101 tcagcgggaa gggggtatag ttaagtccag cggttatggc atgaggatta ctttgccgat
     8161 cgtctttatc gaacagggcc acttcatcgc cataatactg ttcatagacc agtttaccgc
     8221 caaggtgcgg ccaggcgggt agccagcctt ctgcgcgtac atcccagcca ttggccgggc
     8281 gggcttcata atcgttgtcc agctcaggtg cgctgcgcca gttggtcagt cgcaaatagc
     8341 cgttactgct taattttaga tagtcgcgcc agtactccgc gccaatgccg gcgcgggagt
     8401 ggtaacggct aagatcgtgg tcgaaaaaga agttgatgcc cgacatccat gtgggagtga
     8461 aatgacgcca acctaagccg ttgttaatct gcgtacgctc gtcagtacga tggagagtat
     8521 gctgactgaa aaagagatta tcaggcgttt cataccacgg atggagaaaa tcgaactggg
     8581 agttcttcag gctaaaatct tcatccacgc ccagcgtgat tcttgcggta ccgaagcggc
     8641 ttaaccagtc tgtcattgcg cctgaagcct gagaagaggc ccatccacgc gccatatttg
     8701 ccgcttgctc gctgttcata tcttcggcga gcagagaccc gatttgctgt gaagtactgg
     8761 ctatctgttg ctcaaggttg ccactgctat tacccggtgg cggggttaaa ttattttcac
     8821 taacttgtgc cgggacatcc agttcatcac cctggcggac attatcaaaa cctcgagcaa
     8881 acgtacgaaa ctggttgagt ttgcgtaact cagccaccga aataccgaag cgttcggcaa
     8941 cgctttgggc cgattccagc gctccaaggg tatagggtac cgtattggca tttgcaatgg
     9001 cgaattgtgc aggaactggt tgttgggttg cggcgtttac cacaccttgt gctgccgcag
     9061 ccatagggaa cgcaagttga gttatcaggc agatacccgc agttaagcgg cgtagtttaa
     9121 ttcccatccc gcataatatt tgtcggtcat ttatttcttc tccacttctc ttcttcgtag
     9181 ccatgcttac cttccctgaa cgagctaaca taaatgcgcc ctttctagca cagcgaaaaa
     9241 atgtaaatcc tgatagcaat atgattgaaa attaataact tttcgttagg cagttttgag
     9301 tgtgagttgc aagaggggag actactgaat aactcaagtt ttataatcga ggggaaaatg
     9361 gtgatggcgt tcatagcaaa acgccctcaa ccataaaggt cgagggcgct taagatgtta
     9421 aaaacccgct atccgttaaa aaacaatgtt caactaaggt cagtgacatt gcgctaaaaa
     9481 agcgaatttt tagaatttgg ctcctctgac tggactcgaa ccagtgacat acggattaac
     9541 agtccgccgt tctaccgact gaactacaga ggaatcgtgt gaacggggcg catattactt
     9601 agcggtacct tgtctgtcaa cactaaattt cataagtcat ttcaattggt taattaatct
     9661 gcaaagttgt taattaatga acattcgtcg ccgaaaacga gtctgtatca ttagtgcgat
     9721 gcagtctctg caaaggatct tgttgataaa attggcagaa acgttgccac aatgaaggga
     9781 aacgaggagc aaaaagttct ggggcgctaa agaaatattc agaaagtacg gcaaaacatt
     9841 cagcaggatc actggcagca taagcatcaa tgctcgccgc attctcgcca actaattcga
     9901 tttcttcctg aatgttgttc attgcagcac gaagatcgtg ttcccagcca gcaacctcac
     9961 gcaacggaat aaagggaact ccgctggcgc gatcgccgtt acgggtgtcc agcttatgag
    10021 cgacttcatg aataatcagg ttaaaaccag aagcatcaaa agaatcttgt atatccaacc
    10081 agttcaaaac gataggccct tgctgccagc tctgacctga ctgaacaata cgttggttat
    10141 gcaccagacc gatatcgtct tcccattcat catcgaccac aaatggcgca ggataaatta
    10201 agacttcatg aaaaccatcc agccattcca gtcctaactc cagaacgggt aggcaaaata
    10261 gaagtgctat ccggcagctt cttaatgaat ccagttcaaa gccctgtaaa ggaacaagcc
    10321 gcttttgctg taaaaaacgt tcggcaagag tgactaattt gctttgttcc tgttccgtca
    10381 gacacgttaa aagggggatc gatagtgctt cctgccaggg aagggcagtt tgatgtgctg
    10441 attcttgtac tttccagggc cacttaatca tcgttttgct cgcaaactcg tcacttgaac
    10501 aaaattgcac ggacagggac tgttaaaatg ccaaatttcc tggcatcatg gcaaccatct
    10561 gaacggagag atgccggagc ggctgaacgg accggtctcg aaaaccggag taggggcaac
    10621 tctaccgggg gttcaaatcc ccctctctcc gccactttat caaagactta tctcccaact
    10681 tcccgccttg cttttcctaa acagaacaat cgtagaatat tcttgaaggg ttagatcgtc
    10741 actgttttct gttcgatact gtgacattca gcacttgatt cgctatggat ctgacaggaa
    10801 ggttttgagc gaaaatctgc agttattcag tcgttttctt atcggtcacc attattcttt
    10861 tagacattga tcctacaaag ctgccgcaaa gt
//
LOCUS       DAZFRN010000159        10819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFRN010000159 DAZFRN010000000
VERSION     DAZFRN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.161"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            1..825
                     /gene="hlyE"
                     /locus_tag="ACHKUB_004107"
                     /pseudo
     CDS             1..825
                     /gene="hlyE"
                     /locus_tag="ACHKUB_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(1032..1478)
                     /gene="ycgN"
                     /locus_tag="ACHKUB_004108"
     CDS             complement(1032..1478)
                     /gene="ycgN"
                     /locus_tag="ACHKUB_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO6657058.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLVEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1570..2229)
                     /gene="ycgM"
                     /locus_tag="ACHKUB_004109"
     CDS             complement(1570..2229)
                     /gene="ycgM"
                     /locus_tag="ACHKUB_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO6657059.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2301..2594)
                     /locus_tag="ACHKUB_004110"
     CDS             complement(2301..2594)
                     /locus_tag="ACHKUB_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO6657060.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2606..2764)
                     /locus_tag="ACHKUB_004111"
     CDS             complement(2606..2764)
                     /locus_tag="ACHKUB_004111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657061.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            2835..3236
                     /gene="pliG"
                     /locus_tag="ACHKUB_004112"
     CDS             2835..3236
                     /gene="pliG"
                     /locus_tag="ACHKUB_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO6657062.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3356..3724)
                     /gene="ycgJ"
                     /locus_tag="ACHKUB_004113"
     CDS             complement(3356..3724)
                     /gene="ycgJ"
                     /locus_tag="ACHKUB_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO6657063.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4244..4939
                     /gene="minC"
                     /locus_tag="ACHKUB_004114"
     CDS             4244..4939
                     /gene="minC"
                     /locus_tag="ACHKUB_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO6657064.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4963..5775
                     /gene="minD"
                     /locus_tag="ACHKUB_004115"
     CDS             4963..5775
                     /gene="minD"
                     /locus_tag="ACHKUB_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO6657065.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5779..6045
                     /gene="minE"
                     /locus_tag="ACHKUB_004116"
     CDS             5779..6045
                     /gene="minE"
                     /locus_tag="ACHKUB_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO6657066.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6417..6761)
                     /locus_tag="ACHKUB_004117"
                     /pseudo
     CDS             complement(6417..6761)
                     /locus_tag="ACHKUB_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6796..6915)
                     /locus_tag="ACHKUB_004118"
     CDS             complement(6796..6915)
                     /locus_tag="ACHKUB_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO6657067.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6876..7061)
                     /gene="ymgJ"
                     /locus_tag="ACHKUB_004119"
     CDS             complement(6876..7061)
                     /gene="ymgJ"
                     /locus_tag="ACHKUB_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO6657068.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7162..7335
                     /gene="ymgI"
                     /locus_tag="ACHKUB_004120"
     CDS             7162..7335
                     /gene="ymgI"
                     /locus_tag="ACHKUB_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO6657069.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7337..7681
                     /gene="ymgG"
                     /locus_tag="ACHKUB_004121"
     CDS             7337..7681
                     /gene="ymgG"
                     /locus_tag="ACHKUB_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO6657070.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7685..8020
                     /gene="ymgD"
                     /locus_tag="ACHKUB_004122"
     CDS             7685..8020
                     /gene="ymgD"
                     /locus_tag="ACHKUB_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO6657071.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8077..10398)
                     /locus_tag="ACHKUB_004123"
     CDS             complement(8077..10398)
                     /locus_tag="ACHKUB_004123"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6657072.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFLATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10515..10724)
                     /locus_tag="ACHKUB_004124"
     CDS             complement(10515..10724)
                     /locus_tag="ACHKUB_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657073.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAMAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT         3005 a         2589 c         2367 g         2858 t
ORIGIN      
        1 atatctcgat caggtcatcc cctggcagac ctttgatgaa accataaaag agttaagtcg
       61 ctttaaacag gagtattcac aggcagcctc cgttttagtc ggcgatatta aaaccttact
      121 tatggatagc caggataagt attttgaagc aacccaaaca gtgtatgaat ggtgtggtgt
      181 tgcgacgcaa ttgctcgcag cgtatatttt tctatttgat gagtacaatg agaagaaagc
      241 atccgcccag aaagacattc tcattaaggt actggatgac ggcatcacga agctgaatga
      301 agcgcaaaaa tccctgctgg taagctcaca aagtttcaac aacgcttccg ggaaactgct
      361 ggcgttagat agccagttaa ccaatgattt ttcagaaaaa agcagctttt ccagtcacag
      421 gtagataaaa tcaggaagga agcgtatgcc ggtgccgcag ccggtgtcgt cgccggtcca
      481 tttggattaa tcatttccta ttctattgct gcgggcgtag ttgaagggaa actgattcca
      541 gaattgaaga acaagttaaa atctgtgcag aatttcttta ccaccctgtc taacacggtt
      601 aaacaagcga ataaagatat cgatgccgcc aaattgaaat taaccaccga aatagccgcc
      661 atcggggaga taaaaacgga aactgaaaca accagattct atgttgatta tgatgattta
      721 atgctttctt tgctaaaaga agcggcaaaa aaaatgatta acacctgtaa tgagtatcag
      781 aaaagacacg gtaaaaagac actctttgag gtacctgaag tctgataagc gattattctc
      841 tccatgtact caaggtataa ggtttatcac atttaaaaac acagacgttt tttaaagatt
      901 gagagttacc tgttgataat aaggtatcgg gtaactctca ttatgacaga gcgctttcaa
      961 aaacagggct acgaaatggc gagcatccag acgtatatta agaaagcccc catcaacatc
     1021 tattaaattt atcactgcgc ccagtcaggt ttatttaaga tatgatcctg ccagtcaatc
     1081 acttctgatt ctttcactgc gatatgacgc acagagatac gttcaccatg cattgccgct
     1141 ttcgaacccg taagtagcgg atgccacgca ggtaaatctt taccttccac cagcaaccga
     1201 taagcgcagg tcattggcag ccattcgaat gttggcagat tttcacgggt taatttaatg
     1261 cagtcgggtt caaactcgaa acgacgttcg tagttccgac attgacaggt tttaatattg
     1321 agctggcgac aggcgacgtt agtgaagtag atttcgtcgg tgtcttcatc catcagttta
     1381 tgcaggcaac actgaccgca accatcacac aacgactccc attccgcatc gctcatttcg
     1441 tccagggttt tactttgcca gaaaggtaca tcgctcatca ggtgttccgc cattacgtta
     1501 aaacgcacct tataaccagt ctggcacagc gatgcaagtt ttgccgccgc tttcaggcgg
     1561 caaaaagtat tacaaaacgc gagttgccaa agaatgccca tcgaaagtga ctgtcagctc
     1621 atcaccgctt tgcaacgggc cgacgccatc aggcgtgcct gtcagcacaa cgtcaccggc
     1681 cttgagggta aaaaacttgc tcatataagc gatcagcgga acaattttat ggatcatgtc
     1741 cgcagtcgta ccttgctgac gttgctcgcc gtttacgctc aggcccagcg ttgtattttg
     1801 cgggtcgccg gtaaattccg ccgcgggaat aaacccggaa agcggacaag agttatcaaa
     1861 cgctttagcc ttttcccacg gctgcccggc tttcttcatt tttccctgaa catcacgcaa
     1921 tgtcagatcg agcgccacgc cataaccggc aatggctttg cggacatgct cttccgtagc
     1981 ctgacgcagc gtcgcgccaa tcaacaccgc cagttcgact tcatgatgaa ctgaaccgaa
     2041 atcggatggg atcgccaatg gctgccgcag atcgcacagt gccgtttctg gtttaataaa
     2101 cagcactggc tcttcgggca ctgcgctgcc catctcttta atatgtttgg catagttact
     2161 gccaacacag actactttac tcaccggata atccagcagc gcaccttgcc agttgtgatg
     2221 ttgatacata acgttcccct caccttatcg tggatatgaa aacccgcgcc gacaaagata
     2281 atgccaccgc cggatatcgg ttatttgtta gtgtcgtctg ttttctgccc catgacggaa
     2341 agatgttgct tcagcaaatc ttcgggtggt ggcggtaact gcaaatagta accttgctcg
     2401 gttaatgcca gtttaacctt ttcaatatca gcattgacca gtttcttccg cccatccaac
     2461 ggcagaatca tcgctaactg aggctgacca aaacctttca tcagttcctc aggaacacgc
     2521 gaaaaatcgt cttttttttc gacatataaa taggtctggt cacgcttgct gcttcgataa
     2581 atcacacaaa acatactttt actctttaag atgccaggtt ttggcataag ctaacatcaa
     2641 tgaaacccgc gccacaagcc agccagctgt agatttctac ggtttgttga gtccatgccc
     2701 ctttataagt aatccccccg gcaaaaatgc cgcgccggga ttttaccgaa cgcaggaaag
     2761 ccactggaat tctcccgtta gggattgtta tattataaca gttcatcgta ctcattctga
     2821 acaggagact accaatgaaa atcaagagca tcagtaaggc tgtattgctg tttgcactgc
     2881 taacctccac gagctttgct gcgggtaaga atgtcaatgt tgagttcaga aaaggccata
     2941 gcagcgctca gtactccggc gaaataaagg gatacgatta cgatacatat accttctatg
     3001 ccaaaaaagg tcagaaagta catgtgagta tctctaatga aggcgctgat acttacctct
     3061 tcgggccagg aatcgatgat tctgttgatt tatccagata ttccccagaa ctcgatagtc
     3121 acggacaata ctcactccct gcttctggca aatatgagct gcgagtgctg caaacccgaa
     3181 acgacgcccg taaaaacaaa actaaaaaat ataacgtcga tattcaaatt aagtaaatgt
     3241 caaccgacgg ggactctccc cgtcataaac gttaacgccc cactcctctc tgtcgctcaa
     3301 gaggttaatg acgaatagca gacaatgccg aatagaacat aatgtaaaca aggcatcact
     3361 cacgacacat aaataacatc tttgtggtga tttgattgat ttttccactc cgcttaccat
     3421 ccacaccaaa ataacgatca tcacgacata atttttcttt aacatcgcaa aatacaccgt
     3481 tagcgaatgt aaattcgctg ggatcgtagc cttgtaatga ttgtaagttt tcagcggctt
     3541 tttcgccgag atatttcctt gttaactctg gggaaatgcc atctgagttc acacaaatat
     3601 acgggtcgca aacaacgccg cacgccggag attgaagtcc gccagcgtca tgtccagacg
     3661 ccattgatga gaacatcatc cccacacctg acaatccgat ataaaaaata cgcatcatat
     3721 tcatagcaca tcctgataat ccaaccgtac aacacttatg ctcatttcat catcaatccg
     3781 taatcaaaac cgctgacctg gacgagttac gatgacctga cagaagtatc agacgtcttc
     3841 tgcggtttct atgatagagc aattctgtgg cggatattaa gataacgggt atctgttctt
     3901 taatgatgcc gcaaaaaatg gaaagttaag aaagcctggc gtggtgaaaa aatacacttg
     3961 cctcaatact ggatatttaa acaggtctgg agatgctgtc acgattaggg tgcggaaaaa
     4021 ggaagcgtaa ataagaacaa tcaaaagtac gacggcaatg ggttgattga caagggtatt
     4081 ttttaagcta tgaatcagcg ccatttatca cagaatagac ttttactctg aataaatggg
     4141 agggtgactt gcctcaatat aatccagact ataacatgcc ttatagtctt cggaacatca
     4201 tcgcgcgctg gcgatgatta atagctaatt gagtaaggcc aggatgtcaa acacgccaat
     4261 cgagcttaaa ggcagtagct tcactttatc tgtggttcat ctgcatgagg cagaacctaa
     4321 ggttatccat caggcgctgg aagacaaaat cgctcaggcc cccgcatttt taaaacatgc
     4381 ccccgttgta ctcaacgtca gtgcactgga agacccggta aactggtcag cgatgcataa
     4441 ggcggtttcg gcaaccggtt tgcgggttat tggcgtaagt ggctgcaaag atgcgcaact
     4501 taaagccgaa attgaaaaga tggggctgcc tatcctgacg gaaggaaagg aaaaagcgcc
     4561 gcgtccagct cccgcaccac aggctccagc gcaaaataca acgccggtca caaaaacgcg
     4621 tttaatagat accccggtgc gttccggtca gcgtatttat gctccacaat gtgatctgat
     4681 tgttacaagc cacgttagcg ctggggccga attgattgcc gatgggaaca ttcatgtcta
     4741 tggcatgatg cgcggtcgtg cgcttgcagg ggcaagtggt gaccgggaaa cgcaaatatt
     4801 ttgtacgaac ctgatggcgg aactggtgtc catcgcaggt gaatactggc tgagtgatca
     4861 aatcccagca gaattttatg gcaaagcggc gcgactgcag ttagtcgaaa acgctttgac
     4921 cgttcaaccg ttaaattgat ccctttttaa caaggaattt ctatggcacg cattattgtt
     4981 gttacttcgg gcaaaggggg tgttggtaag acaacctcca gcgcggccat cgccactggt
     5041 ttggcccaga agggaaagaa aactgtcgtg atagattttg atatcggcct gcgtaatctc
     5101 gacctgatta tgggttgcga acgccgggtc gtttacgatt tcgtcaacgt cattcagggc
     5161 gatgcaacgc taaatcaggc gttaattaaa gataagcgta ctgaaaatct ctatattctt
     5221 ccggcatcgc aaacacgcga taaagatgcc ctcacccgtg aaggggtcgc caaagttctt
     5281 gatgatctga aagcgatgga ttttgaattt atcgtttgtg actccccggc agggattgaa
     5341 accggtgcgt taatggcact ctattttgca gacgaagcca ttattaccac caacccggaa
     5401 gtctcctcag tacgcgactc tgaccgtatt ttaggcattc tggcgtcgaa atcacgccgc
     5461 gcagaaaatg gcgaagagcc tattaaagag cacctgctgt taacgcgcta taacccaggc
     5521 cgcgtaagca gaggtgacat gctgagcatg gaagatgtgc tggagatcct gcgcatcaaa
     5581 ctcgtcggcg tgatcccaga ggatcaatca gtattgcgcg cctctaacca gggtgaaccg
     5641 gtcattctcg acattaacgc cgatgcgggt aaagcctacg cagataccgt agaacgtctg
     5701 ttgggagaag aacgtccttt ccgcttcatt gaagaagaga agaaaggctt cctcaaacgc
     5761 ttgttcggag gataagttat ggcattactc gatttctttc tctcgcggaa gaaaaacaca
     5821 gccaacattg caaaagaacg gctgcagatt attgttgctg aacgccgtcg cagcgatgca
     5881 gaaccgcatt atctgccgca gttgcgtaaa gatattcttg aggtcatttg taaatacgta
     5941 caaattgatc ctgagatggt aaccgtacag cttgagcaaa aagatggcga tatttctatt
     6001 cttgagctga acgtgacctt accggaagca gaagagctga aataagcccg ctgtaaaaac
     6061 gcatttatct tcaaggcaga gtcatttctg ccttgagttt ttcatccctc tcatccacgt
     6121 tgtggtaaag cggcgagtat tcttgctgat actcctcatt gctatttcca cctccccctt
     6181 tacaccttaa ggctgttccc acgcacagaa caaaatgttt atgcctggtt gagtaaataa
     6241 ccctgttgtt agtatggata tactgggaga tgataacccc gcaatttcat tattcattgt
     6301 tcccccatag gatgaggttt cccgccgtaa ctggcgggct ttttttgccc cagaattttc
     6361 tctttcaaca tcctgtaaac gaaaactgcg ccgaagcgca gtttaatcgt tgatggtcag
     6421 aacgaccagt tcacaccagc caccgcgttc cacggggact ccacaccggc accatggcta
     6481 taccccaccc cgaaatgccc gcttaacgta ctgctgaatg aggctttaat acctgcctgg
     6541 tatattccac gtctgcccga caaatcattg acaaaattac cgtcactatt cactttcacc
     6601 cgattatcat cgacaaattc tttgcgcaca gccgccttca ggcagggttc aatttccata
     6661 ccgttcccca gacgcatgtt gtaactcagc gttgcaccca gttcacgata tatactgcgg
     6721 gtatcgactg atttcgattc catgccattg gataaatgat agtagcgtct aagtgaccta
     6781 agctctgctg caacatcagc ccgcaggcac cagaccaggg gattcatcct gaagagacag
     6841 cgcaggtgta ttgtgttcac cgctcaccaa agacatcatg atgaaatgat gatatcccgc
     6901 ataagaaaga ggcatttttt acacgcagtg cgctgaagtg tagttggata aaaaatcact
     6961 cttttcagat aatactgtcg tacgcttatc tttcgacaat gaggccgtcg gcaaaataaa
     7021 atgactaatg taatcgttgc tgatgaatat cttctgctca cataaaaatc acacaataat
     7081 attgataaat cagattatct tacctttaca acattgcgtt aacagtcaat gacacttctt
     7141 gaataaaaat attgtcaatc aatgagttat tcctcattta agattattct cattcacgta
     7201 aaaaacataa ttcctatcat cacagccacg ctcattttga attatctgaa taatagcgag
     7261 cgttctttag ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc aacttactta
     7321 ataccggaca tctaagttga agaaaaaaat acttgccttc gggcttatct cagcattgtt
     7381 ctgctccacg ccagcgatgg cggatatgaa ccgcaccacg aaaggtgcat tgctaggcgc
     7441 aggcgttggt ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg
     7501 cgctggtgtt ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcgc gtaaaggtgc
     7561 aaaagtaggt gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg
     7621 ggcgattaaa ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata
     7681 aggaatgatg atgaaaaaat ttgctttact ggcgggcctg tttgttttcg caccaatgac
     7741 ctgggcgcag gactacaata ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc
     7801 agaagccaac gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtaat
     7861 gggtaatgcc agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc
     7921 caaagtggta gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac
     7981 ggcaattgat gacaccatgc gtgctatcgg caaaaaataa tatttcatat gtaccgcgtc
     8041 gatgaatacg acgcggtttt ttagctaacg gcgggatcag aacttatatt tcaggcccac
     8101 catcactgcg gtgtcattgt agccattatc acccagctgc acgcccacat tcccccagac
     8161 gctggccgct ggattcaggt tgccatttac gccaagttta atttccccca aattactcgc
     8221 cccatcttgt tcgactttca caccgttcat tgagacagca aaatctttac tgttatggat
     8281 ccagttcatt tcaacaaaag gttcaaagtc gtcaccgtgc ggaccgctgt ttttctcctg
     8341 agtacgaata aaggtgcgga aaccaagtcg ggtctggatg ttgttatttg cgtcattctc
     8401 aatacgtgag ccattggctt cggtgtgcgc attatgatcg acgcccatcc agatagcctg
     8461 tgcctgtggc tggaatatcc acgtgtattt cgccgcttcc agaccaaagg atttattcag
     8521 ggcaaagccg taacccgctt ccagcgagac ggttgcgcct ttcgcactat aggattcata
     8581 ggctaattca tcccctttca ccgagttttt aaaccagcta tattgcgccc aggcgtcaat
     8641 gtatgcgcct tttttactga tgtcatcggc aaaccaagtg gcatacagcc ctgcgcaata
     8701 gcctctgaca ctccctttcg aacgataatc cgacacgctg gaatgagttg agttgtagtc
     8761 gcgggcataa ccagccatca ctcccaaacg ccaactgtca ctgtcggtaa aaccaccggt
     8821 taacagctcg gcccctaact gcgagacgta gcgatgcgag gtcgttctca actgtccgtt
     8881 gctgtcacgc caggcattat gcccgccaat ttgacgtagc caaagtcggc ttgaacgctc
     8941 ctgttcagtt acaggatcga tgtagcgcat ttcacccgca cggtcgttca gacgcataac
     9001 aaaaagagtg ttagcggctg ccaggttggc gacgtaactt ccagcttcga ggcgcaggac
     9061 gggggtaggc gcaacagtag gcgctgaact tcctccggag tttgtttcac cgccgctcgt
     9121 tgccaggaag ttagtcagat accagttttt attggtcgct tcggtattac ctcgggttaa
     9181 tgaatattcg taggcaccgg cgaaaatcct cgaatcctta ataaattccc catttgattc
     9241 cccctcaacg ctgataatct ctatcccatt taacgtgtaa gctccgctgc ctcctgcatt
     9301 ggtaacttta actcgggttg ttccagaggt atttccatta ataacgactt tatccgtagc
     9361 tgaattatca tcacccaatt cagttctgag atgcaatacg ccattattac caacgtaatt
     9421 ttcagttatt gttaatcttg ttggttcgac atcccgtcca tctgcccggg aaatatagac
     9481 cgtactatta tcaaccgtga ggttgctcac cgttgagtca gctttaatat cccaggtact
     9541 attatctgaa agcgacagat aggtatgagt gttatcatct gttgaaatat tagctgaacc
     9601 cgttattttt gaattagttg catcgacggt caaatgacct ttaatattat tgataaaaac
     9661 atctgcagcc gatgatacct cactatccgt aatggtcacg ttactcgtct catttttatg
     9721 gtagaggatt tcatttgctg accgtagtga gctaccagat aaaatatcta ccgttgcttc
     9781 ctgaccattt atggaagact ccagcacaat tccagacccc tgatgcgcga ctgcagatgt
     9841 atttttcagc gtcaccgtgc cgccattata aattttagca atcgcggcgt caggacccgt
     9901 tgtataaatt tctccctggt taaatgaaac tgtcccctgg cgggcagcga tggcatgagc
     9961 attgtcccca tgcgtggtga tatcaacatc ggttaatgta acgctggcat tggtataatc
    10021 aagcgtgtca ccagccttga cgacagtgcc attagcgtac gcggcataac tttctgttcc
    10081 gtgggttgtg atctcccctt ttgccatatc cagttttgct atggcattat tttcattact
    10141 tcctgtatcg gtagtacttt cttcatcaag agaagataca cacgcacgcg cgcttccggg
    10201 agatgcacac gcaagaacgc cgactgcgtg attacccgca gtaacaataa atgtatcatc
    10261 tccgtcagaa aaagaggggg taaccgaacc gtcagagttg actcttatta tggtgtcagt
    10321 attcaggttt ccactcgttt gacttagcaa accaattgag taatcccccg ttgtgacaat
    10381 cgtgttttta ccctgcaaaa taacagcacc gccaaattca cttacgattg cgctagctgt
    10441 taatgctgtg cttagaggac tattgtcaga aggatataaa ccgcccgttg ttacaatgac
    10501 gccattgtta atggttaact tgctaccctc tcctgcaacg gatacccgta acttccatta
    10561 cccgttgtta taactagcgc attatcaata tcttttactt ttccatcttt tattgccata
    10621 gcatgtttat attcatcgtg ctgaataaac tgttcagcat atgcattacc caccgccaga
    10681 gcaataagtc ccaaactaaa cccggagagt tttttcagtt tcatagatta accatccatt
    10741 cattggatta acaaacattc ctggtcaaat aacctatgcg ttcagattac caaaacggag
    10801 ggtaaagaca aaaacaaaa
//
LOCUS       DAZFRN010000160        10790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFRN010000160 DAZFRN010000000
VERSION     DAZFRN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.162"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(62..808)
                     /gene="tia"
                     /locus_tag="ACHKUB_004125"
     CDS             complement(62..808)
                     /gene="tia"
                     /locus_tag="ACHKUB_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion outer membrane protein Tia"
                     /protein_id="HFO6657074.1"
                     /translation="MKKVIVVSALAMAGVFSAQALADRGKTGFYVTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSHY
                     TLDTWHSPMGDGGREDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARIHHKAT
                     YTDTSWNKSGEVSDISALHYSGYDNNFAWSIGAGVRYDITPDIALDLSYRYLDAGKSS
                     LSYKDAEGDKYKSEADVKSHDIMFGVTYNF"
     gene            1031..1216
                     /locus_tag="ACHKUB_004126"
                     /pseudo
     CDS             1031..1216
                     /locus_tag="ACHKUB_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1410..1703
                     /locus_tag="ACHKUB_004127"
                     /pseudo
     CDS             1410..1703
                     /locus_tag="ACHKUB_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_141174869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2405..3361
                     /locus_tag="ACHKUB_004128"
     CDS             2405..3361
                     /locus_tag="ACHKUB_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HFO6657075.1"
                     /translation="MAVSIAEYLGQRTDVDQQIVPVAKGTQIQCPFMDRTCDKASKVK
                     NPTPPVCSVRKPDGTVWIVCEHRLCSTRQKKTVIVNGRKKQIENILVEHQRDILRKVA
                     KLIYQDPELQDSEIGVRREVNIPLPDSDNSYHADYVMRNFSGRGRVDEVLLEMQGGGE
                     TSSTGEITRHIAAWADLEFPTNEILRQPVAANTIETNAWRRQQEQFLVKGNVVDQTGG
                     KIVFAVGSLLYDYLHRRFRNANLRDLKAHNWTLCILAFKEDTSDEPRPGPIPLIIDDS
                     KTLFTNYSTFVRFLTDQGAPRPELFEGSFLRLDNSSVTIMPR"
     gene            complement(3342..4271)
                     /locus_tag="ACHKUB_004129"
     CDS             complement(3342..4271)
                     /locus_tag="ACHKUB_004129"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO6657076.1"
                     /translation="MKKKPLKQYKVSSFFAGIGGFDLGLEKAGMEVVFQCEINKFCQK
                     VLRKNWSKVPLHTDITRLNADEIPESNVWCGGFPCQDVSSANQGKRKGLEGARSGLFY
                     TYAKLIEERKPEWLIIENVPGLLNSHNGQDFKVVIDTLVEFGYGVSWRVLDAKYFGTP
                     QRRRRVYIVASLGDMRSARVLFEPGATRIVDKQGQGERANASGASATGLSKADIYSIQ
                     HASIGRKATAGPQAKGYRNDGETYTLDSRGSSDAVCAPDAAFRVRASTGISRELDSNR
                     FRAVGNAVAVPIVEWLGKRIVLVDQQVLNEALS"
     gene            4725..5099
                     /locus_tag="ACHKUB_004130"
                     /pseudo
     CDS             4725..5099
                     /locus_tag="ACHKUB_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611478.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            5261..6000
                     /locus_tag="ACHKUB_004131"
                     /pseudo
     CDS             5261..6000
                     /locus_tag="ACHKUB_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    5542..5658
                     /locus_tag="ACHKUB_004131"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            6673..8181
                     /gene="ltrA"
                     /locus_tag="ACHKUB_004132"
     CDS             6673..8181
                     /gene="ltrA"
                     /locus_tag="ACHKUB_004132"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO6657077.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            8262..8750
                     /locus_tag="ACHKUB_004133"
                     /pseudo
     CDS             8262..8750
                     /locus_tag="ACHKUB_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8833..9216)
                     /locus_tag="ACHKUB_004134"
     CDS             complement(8833..9216)
                     /locus_tag="ACHKUB_004134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657078.1"
                     /translation="MDIEITNPVNIKLDVIEFDEHVPSLKFSVSISVEKFGYKAEVNT
                     HFWIECQCFDKFVSCMNCGELAILKDMNDSFELILNPVAGVLNWSCMKEDINGGISLS
                     KGSEKLDDAAKQSILRAFNDYPKWW"
     gene            complement(9217..9723)
                     /locus_tag="ACHKUB_004135"
     CDS             complement(9217..9723)
                     /locus_tag="ACHKUB_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004712764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO6657079.1"
                     /translation="MDKATAESYVETYDGMKTVAASMTPLLGAAAVSKIDSIVANYSK
                     ANQLSTGTVFDSIKATQPVHPGSVIPQSFEMSLPNGQKIWVHGNATEYMAEYAASKAV
                     THTPEAVRLASQVELSSFQAAVNTATKTNMPYGARITVDGWQLEIKPPRVDGELPTII
                     HARYLGSH"
     gene            complement(9716..9889)
                     /locus_tag="ACHKUB_004136"
     CDS             complement(9716..9889)
                     /locus_tag="ACHKUB_004136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657080.1"
                     /translation="MNATLAVVQENSALAGAAMGELAVRAIAGMLYPGVKQSDLSEEQ
                     KQTISTLATVRCG"
     gene            complement(9927..10448)
                     /locus_tag="ACHKUB_004137"
     CDS             complement(9927..10448)
                     /locus_tag="ACHKUB_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO6657081.1"
                     /translation="MSHDAEKKIRLCIQCENEALKDSDFCAVCEEREFKKIRGWLYLP
                     AIGLVLTILANMISINFTIRFLMENIAQIGHAQKGILFFELFAFIGMFSYGIFVSSLF
                     FRKKRQLPHHYIALIGIGTIFVAVDLLLGHIYLDVPYVFDTVKPLVRNVFSACIWIPY
                     FIVSERVKRTFVK"
     gene            complement(10463..10714)
                     /locus_tag="ACHKUB_004138"
     CDS             complement(10463..10714)
                     /locus_tag="ACHKUB_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin domain protein"
                     /protein_id="HFO6657082.1"
                     /translation="MLNMIATETAANTAAAIAGATGNASKDTGNDNSLDTGGGCEGSR
                     EQCAVRDPSRDGTRGPGHMLNGDNTDENKQHDLQLFPYK"
BASE COUNT         2969 a         2566 c         2549 g         2706 t
ORIGIN      
        1 agaagatata taaatatctt aattactgaa tatgtgtaat tatcgctcag gagcagtgtc
       61 gtcagaagtt ataagtcaca ccaaacatga tgtcgtgact tttaacgtca gcctctgatt
      121 tatatttatc cccttctgca tccttgtaag acagggagga tttaccagca tccagatagc
      181 gatagctgag gtcaagagcg atatccgggg ttatgtcata gcgaacaccg gccccaatgc
      241 tccatgcgaa attgttatca tatcccgagt aatgcaacgc agaaatatcg ctcacctcac
      301 cagatttatt ccaggaggta tcagtatatg ttgccttatg atgtatccgt gcataaccca
      361 gtccaacaga cacccatgga gtgaatgcac tgctgtttct gaagtcataa tacgtattca
      421 ccatcagggt attcacactg agcctgttct gtgtgtcttc ccgaccacca tcccccatcg
      481 gagaatgcca tgtatccagt gtataatggg agtctgcagc tccgcggcca tagaattcca
      541 cttccgtgcg cacaggaaca ttgtaatgtt gataaaaatc atagcccacc gcaaggcctg
      601 caccaaatac agtatcgttt ttatcaccgc ccttatactt ataaacatca tccccaaagt
      661 cctgacggaa gcgctggtca gtctgagtca caaccgaagc accagcttta ccggtcacat
      721 aaaagcctgt tttaccccta tcagccagag cctgggctga aaatacgccc gccatagcaa
      781 gcgctgaaac aacaataacc tttttcattt caatcatctc cgttggttct tacccgcacg
      841 ataaagaagg ttgcttattt taaaaccatg caaaaaacag ggtaaaaaga gaaaattcga
      901 tcgaatcgat cgacaaaacc gatcatgcat gatgaagatt tcttatcgaa gacatgaaaa
      961 tggtaaggaa tggtttaaga ggtaattgca acggcatgga agtattgaga ccagaacagc
     1021 acgagataaa gcggctgaac gtcagatgag ccacatggac gtaactcagt gcaggccgtg
     1081 tataatccat aaagcggaat atctggataa gcgtaagctg atgttgcagt ggtgggtcga
     1141 ttttctggat gccaatcggg aaagagcgat tacgccgttt gactatgcga agatcaatcg
     1201 gggtaatggc gagtgagttg aatgccgtta ctccttggct gatgtttaaa tttaaaagac
     1261 caaccagacc gcttaggact ggttggtcgc tgtatttcat ctgccaggta cagagccgtc
     1321 aggcaaaaag gatgtctgca ataatgcctt gaatatacat atagttgcga gcatcatcgc
     1381 aaacatatgc tcatgaattc ttcaacgcaa tgacctctga caaaatccta aagcaagcga
     1441 tatcaaatat caccatctgg cgcaaaggtg aacaacgtgc gccacataag ccattattgc
     1501 tgctgtacgt tctctcacac tatcggcagg gtcatggtcg cctgtttaac tatgcttctg
     1561 aaatctacga gcccctgttg gatctcctgg aacgttatgg gccgcagcgc cgtgaccagc
     1621 gcccggacat gccgttctgg cgtctgaagg gcgatggctt ctgggaacta cattacgcgg
     1681 aactgtgctc aaccagtggt aacactgagc acgcagttca tccgtcaggc gtggtgcacc
     1741 gtaacgctgt tttgaccggg taaaagccgc gaggacaacg ctgtgcagga aatcgtccgc
     1801 atccccataa cggtcaaaca tcgtggcgtt gacattgtgc atctgtgcca gtaaaccgca
     1861 atttaactcc tctttcccga tctccataca ttcgctgcca gccaagctac tgcggaacgc
     1921 cactgttatt gcctgattca gcagctattt ggtatccgcg cgtagtccca gtgtcgtaag
     1981 cggctcgcca gaactgagtg gtcagctcaa cttttcaggg taacaggtcg cgcatcctga
     2041 cgggagcaac ccgcaaaatg ccgccatacc atctctgata cgctcatcag ggggagtatt
     2101 gttctgacag cggcaggtcc acagggacaa aactgtaaat cacttcttca taggaatgcc
     2161 cttacgttgc tgatgaaggc atggctaaca ccacggtgta ttaatcaacg gggagcaagt
     2221 cagattggct tcactatctt gggaaataat gagtgcatcg agagatgagc agcgtcaaga
     2281 ctcggacttc cttcattttc atttaaaact tacacctaag atatagaagt tctatgaatt
     2341 aattgacata aattaccact cactatgatc atatcgtata agattcttga ctgaggaatg
     2401 agcaatggca gtgagtatcg cggaatattt aggtcaaaga acagatgtag accaacagat
     2461 tgttcccgtt gcaaaaggta cgcaaataca atgccctttt atggatcgaa cctgtgacaa
     2521 agcaagtaag gtcaaaaatc ctacacctcc tgtatgcagt gtccgcaaac cagatggaac
     2581 tgtttggata gtttgcgaac atcggctatg ttctactcgc cagaaaaaga cagtcattgt
     2641 aaatggtcgt aaaaaacaaa ttgaaaatat tttggttgaa catcaaagag atatattacg
     2701 gaaagtagca aaactcattt atcaggatcc tgaattacag gattcagaaa ttggtgtaag
     2761 aagagaagtc aatattcctt taccagattc tgataatagc taccatgcag actatgtaat
     2821 gagaaatttt tcgggtaggg gaagagttga tgaggtcctg cttgagatgc aaggtggtgg
     2881 agaaacaagt agcactggag aaattactcg acatatcgct gcatgggctg atcttgagtt
     2941 tcctactaat gaaattctaa gacaacctgt agcagcaaat actatcgaaa caaacgcatg
     3001 gagacgacag caagaacaat ttcttgtcaa agggaatgtt gtcgatcaaa ccggagggaa
     3061 aatcgttttt gctgtcggta gcttactcta tgactattta cacagacgtt ttaggaatgc
     3121 aaatctcaga gatctgaaag cccataactg gacattatgt attttagctt tcaaggaaga
     3181 tacatcggat gaaccacgtc cagggccgat cccgttaatc attgatgact caaaaacact
     3241 attcaccaat tattcaacat ttgtaagatt tctaacagat cagggcgcac cacgccctga
     3301 actattcgaa ggatcatttt tacgtttaga taatagttct gttacgataa tgcctcgttg
     3361 agcacctgct gatctacaag gacaattctt tttcctaacc attcaacaat agggaccgcg
     3421 acagcgttcc ctactgctct gaatcgatta gagtccagtt ctcgggaaat ccctgtagac
     3481 gctcgtactc ggaaggcagc atccggcgca catacagcat ctgaacttcc tcttgagtca
     3541 agagtgtagg tttctccatc gttcctgtat ccttttgctt gaggtcctgc ggttgctttt
     3601 cgtccaatac ttgcatgctg gatagaatag atatctgctt ttgataggcc tgtagcagag
     3661 gctccggaag catttgccct ctctccttgg ccctgtttat caacgattct agttgctccg
     3721 ggttcaaaaa ggactcgagc ggagcgcatg tctccaagac ttgcgacaat gtacactctt
     3781 cttctacgct ggggtgttcc aaagtatttt gcatccaata ctctccacga aacgccatac
     3841 ccgaattcga ccaacgtatc gatgacaact ttgaagtctt gtccattgtg gctgttgagc
     3901 agtccgggaa cattttcaat aataagccat tcaggctttc tttcttcgat gagttttgca
     3961 tatgtataaa ataagccgct tcgggcccct tctagacctt tccgctttcc ttggttagct
     4021 gacgacacat cctggcaagg aaaaccacca caccaaacgt tgctctctgg gatctcatca
     4081 gcattaagtc gtgtaatatc agtatgtagt ggaacttttg accaattctt tctaagtact
     4141 ttctggcaaa atttgttgat ttcgcattgg aaaacaactt ccatccccgc tttctcaaga
     4201 ccaagatcga atccaccaat ccctgcgaaa aacgaggata ccttgtactg tttcagtggt
     4261 ttctttttca tatcggcttt ttcatcactt tttgaaccaa ggatcctaac ggaaaccagc
     4321 cccgatggcc accttttttc taattaaagc cactttcttg gtatccaggc attgattatg
     4381 aaataaacta actgtaatta tatacagtat attttaaatg ccaataaaag tgtacttctt
     4441 tgcgaaaagc cttctaattt gactagactt tcgcatagtc aaacgggcta atccctttct
     4501 cgcgacttac atccagaaaa tccactcacc attgcaacat cagtcgctgc atctttcaaa
     4561 tgttccactt tatggatata agcagcccgt acttttttac gttcctgatg actcatctga
     4621 cgtccaacca ctgacgtcca accactcgcg ggactgacct gctccctgtt gattaataca
     4681 ccgcgatgtt agtaatgtct tcataagcta catgaggaca tcccatgaag aagcgttttt
     4741 ccgacgaaca gatcatcagt atcctcctcg aagctaaagc cggggtttct gcccgtgagc
     4801 tctgccgcgc attggatcaa tgagcctttg agcatagtgt cgagttgcgt ttaatccagc
     4861 cgggcaagcc aacgcagaac ggatttattg agagctttaa cggacgattt cgcgatgaat
     4921 gcctgaatga gcactggttc agcgatatcg ttcacgctag gaaagtgatt aatgactggc
     4981 ggcaggatta taacgagtgt cggccacatt cagcactgaa ttatcagacg ccatcagagc
     5041 ttgcagcact atggcgaaat ggaaaatgtg aaggtaacca aaccgacatt actaactgat
     5101 aattgtatct aatactgggg gcaggtcagg attgtcaacg acggatgaaa agtggttctt
     5161 ccgcaacttg ggtggaagaa aatggatgaa actttctggt gaacagtgaa ccgccccgga
     5221 aatcctggag actaaacttc ctgagaaaga ggtaaacagg atgactaaaa aaacacgttt
     5281 ttcccccgaa gtccgtcaac gggcagttcg tatggttctg gaaagtcagg gcgaatatga
     5341 ctcacaatgg gcggcaattt gttccattgc tccaaagact ggctgtacgc cggagactct
     5401 gcgtgtctgg gttcgccagt atgagcggga taccggaggc ggtgatggtg ggcttaccac
     5461 cgctgaacgt cagcgtctga aagagctgga acgtgaaaat cgtgaactgc gccgcagtaa
     5521 caatatcctt cgccaggctt ccgcttattt tgcgaaggcg gagttcgacc gcctctggaa
     5581 aaagtgatgg cactgctgga taagctgcgt gagcagtacg gggtcagacc ggtatgcagt
     5641 gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca tcatcctgat
     5701 aaacgcagtg cacatgcaca gcgcgatgac tggctgaaga gagagataca gcgcgtatac
     5761 gatgaaaatc atcaggtgta cggtgtgcgt aaagtctggc gtcagttgtt acgggaagga
     5821 atcagggtgg ccagatgtac agtggcgcgc ctcatggcgg ttatgggact tgccggtgtt
     5881 ctccggggta aaaaggtccg cactaccgtc agccggaaaa ccgttgccgc aggtgaccgc
     5941 gtaaaccgtc agtgcgtggc agaacgtcct gaccagctgt gggtggctga ttttacttac
     6001 ggtgtgcccg gagttcaggg cgggcatgga tgcttaaatg aaccgcgagt ctgtctggaa
     6061 tattgaaccg gtaactcacg atgagaaacc caacaatccc accgggtgtg acggtggaga
     6121 acctgagcgg cagtgacctg cggcatgccc gcagggtgat gtaacccgct gacaacgggg
     6181 attgaggcga gatcactaag ccgagatgat cctcaaggtt aagtactgaa aggctgaaga
     6241 acatgaaccc gttaatccgc ctctgtgggt tgaaaacgtc accacggcct acgtgatctg
     6301 acaggccgtg caggaggaac tggcagtgat acgtaagcac tgccggtcga aggtgttttg
     6361 acatgtatgc gaaacaccgg ggcagcagcg tctatcacgc ttgcgttgct gacttctgcc
     6421 aacttgcggc aagcaaggat aaagagtgcg acgggcagcc tcctcagtat gcctgagtcc
     6481 aggcaggtaa accggggaag gtcagcgacg gatgttaagg gggcatggct ccgatgacgc
     6541 gctggctggc ggagcttccg tagtagtccg cgatggggaa agcccattac atggcgaagg
     6601 gaagcagttt gaatgtgttt gcgacgtgaa ttaactgacc taacgaggtg aagacctttg
     6661 ataatcagcg aaatgcaacg caagcttgcc acatgggcag ccaccgatcc gtccctacgg
     6721 attcaacggc tgctgcgtct gataacacaa ccagaatggc tggctgaagc ggcgcggatc
     6781 acgctttcat caaagggggc ccataccccc ggcgttgatg gcgtgaacaa aacaatgcta
     6841 caggccagac tggctgttga gctgcaaatc ctcagggatg aattactctc aggccactac
     6901 cagcccttgc ccgccagacg ggtttacatc cctaaaagca acggcaaact gcgaccactg
     6961 ggtatccccg cgttgcggga tcgtattgtt cagcgggcca tgctgatggc gatggagccg
     7021 atatgggaga gtgattttca tacgctctca tatggcttcc ggcctgagcg cagtgtccac
     7081 cacgcgatcc gcacggtgaa attacagctc acagactgtg gtgaaacccg gggacgctgg
     7141 gtgattgaag gcgacctgtc cagttacttc gacaccgtac atcatcgact gctgatgaag
     7201 gctgtacgcc gcaggatcag tgacgcacgt ttcatgactc tgctgtggaa aaccatcaag
     7261 gcgggacata tcgatgtcgg tctctttcgg gcggccagtg aaggtgtgcc acagggcggt
     7321 gttatatcgc cgctattatc gaacatcatg ctgaatgagt tcgatcaata cctgcatgag
     7381 cgctacctga gcgggaaagc cagaaaagat cggtggtact ggaataacag tatccaacgg
     7441 ggccgaagta cggcggtcag agaaaactgg cagtggaaac ccgcggtggc gtactgccgc
     7501 tatgccgatg attttgtcct catcgtcaaa ggcaccaaag cacaggcgga agccatcagg
     7561 gaggagtgtc ggggtgtgct cgaaggcagt ctgaaactca ggctgaacat ggataagact
     7621 aaaatcaccc atgttaatga cggctttatc tttctggggc acaggatcat tcgcaaacgc
     7681 agtcgttatg gcgagatgcg agtggtctca acgatcccgc aggagaaagc cagaaacttc
     7741 gccgcatcgc tgacagcact gttatcaggc aactacagtg aaagcaaagt cgatatggct
     7801 gaacaactca accgaaaact gaaaggctgg gccatgttct atcagttcgt tgattttaag
     7861 gccaaagtct tcagttatat cgaccgtgtc gtgttctgga agctggctca ctggctggcc
     7921 cgcaaatacc gtacaggtat cgcttccctg atgaggtggt ggtgtaaatc accgaaaccg
     7981 ggtcagagca aaacgtgggt tttatttggt aaaaccaatc acggcaagct cagcggcgaa
     8041 atactgtacc ggttggtggg gcaaggcaag aagctgttcc gctggcggct acccgaaggt
     8101 aatccctatc tgaggacgga gaccagaaac acgtatacat cgcgctttac agaagtggca
     8161 atggcgttcg ccagcattta aatggagagc cggatgcgct gaaaggtgca cgtccggttc
     8221 ggggaggaga ggcagggaaa tagtccgact acgccctgcc tcttactcta ctctggatgg
     8281 gtaagcgctg ggcgtacctc gccgttgttc tcgacctgtt cgcaagaaaa ccagtgggct
     8341 gggccatgtc gttctcgccg gacagcaggc tcaccatgaa agcgctggaa atggcgtggg
     8401 aaacccgtgg taagcccggc ggggtgatgt tccacagcga tcagggcagt cattatacga
     8461 gcaggcagtt ccggcagtta ttgtggcgat accagatcag gcagagtatg agccggcgcg
     8521 gaaactgctg ggataacagc cgaatggaac gcttcttcag gagtctgaag aacgaatgga
     8581 tgccggtggt gggttacgta agcttcagcg aggcagctca cgccataacg gactatatcg
     8641 ttggatatta cagcgcacta agaccgcacg aatataacgg tggattaccc ccaaacgaat
     8701 cggaaaatca atactggaaa aactctaact cggtggccag tttttgttga ccacttcata
     8761 cggcggtccg tttacgctta cggtaaagat aagcgatagg ctcccagcgc taggaccggg
     8821 agctctgcat agctaccacc atttaggata gtcgttaaaa gcacgtaata ttgattgctt
     8881 ggcagcatca tcaagctttt cacttccttt tgaaagggag ataccaccat tgatatcttc
     8941 cttcatacat gaccaattca gcacaccagc aactggattt aaaattaact caaaactatc
     9001 gttcatatcc ttgaggatgg caagttcgcc acaattcata cagctaacaa acttgtcaaa
     9061 gcactggcat tctatccaga agtgagtatt aacctcagcc ttataaccaa acttctcaac
     9121 tgaaattgag acgctaaact ttaaggaagg aacatgctca tcaaactcaa taacgtccaa
     9181 ctttatattt acaggattag taatttctat atccatttaa tgagatccta aataccgtgc
     9241 atgaattatt gtcggcaatt ctccatcaac acgaggcggc ttaatttcta attgccagcc
     9301 atcaaccgta attcgcgctc catatggcat attagtttta gttgctgtgt tcacagcagc
     9361 ctgaaaacta ctcaattcca cttggctagc aagacgcaca gcttcaggcg tatgtgtcac
     9421 tgcttttgat gccgcatatt cagccatata ttctgtagca ttaccatgta cccatatttt
     9481 ttgtccattt ggtaaagaca tttcaaatga ttgaggaatt acagatcctg gatgcacagg
     9541 ctgtgttgcc ttgattgagt cgaaaacggt tcctgtcgat aactgattgg cttttgaata
     9601 attagcaaca atagaatcga tcttactgac ggcggcggcc cccaacaaag gcgtcattga
     9661 tgccgctaca gtcttcatcc cgtcataggt ttcaacgtag ctttcagccg tcgctttatc
     9721 cacaccgaac ggttgccagc gtacttatcg tctgcttctg ctcttcactg aggtctgact
     9781 gtttcacgcc cggatacagc attccggcaa tcgctctgac tgccagttct cccattgctg
     9841 caccggcaag ggcgctattt tcctgtacca ccgctagcgt agcattcacc actgcatgag
     9901 ttcatgtgag acatctgaca gacaaattat ttcacaaatg tccgcttaac gcgctcagat
     9961 acaatgaaat aggggatcca aatacaggca ctaaacacgt tacgtaccaa tggttttacc
    10021 gtatcaaaaa cataaggcac gtctaaatag atgtggccca gcagtaaatc gacagcaaca
    10081 aatatcgttc ctatcccaat aagtgcaata tagtgatgcg gcaattgacg ctttttacga
    10141 aaaaacaagc tactaacgaa aattccataa gaaaacatac caatgaaagc aaagagttca
    10201 aaaaagagga tccctttttg tgcgtgaccg atctgcgcaa tattttccat taagaatcgt
    10261 atagtgaaat taatagagat catattggcg agaattgtta aaaccagtcc tattgctggc
    10321 aagtatagcc aaccacgaat ttttttaaat tcgcgttctt cacaaacagc gcaaaagtca
    10381 ctgtccttca gtgcttcatt ctcacattga atacaaagtc ttattttctt ctctgcatca
    10441 tggctcatct gataatcacc tattatttat acgggaaaag ttgaagatca tgctgtttat
    10501 tttcgtctgt attgtcacca ttcagcatat gacccggtcc ccgggtacca tcgcgactcg
    10561 gatcacgtac agcacactgt tcccgtgaac cttcacaacc accaccggta tccaagctat
    10621 tatcattccc ggtatcctta ctggcattac ctgtagcacc ggctattgct gctgccgtat
    10681 tagctgctgt ttcagtggct atcatattca gcatattgtt tccagcaact atcttaagtt
    10741 ccaattaatc atttctttag caagttggca atccataaca ttaaacctgt
//
LOCUS       DAZFRN010000161        10709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFRN010000161 DAZFRN010000000
VERSION     DAZFRN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.163"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1115
                     /gene="yghO"
                     /locus_tag="ACHKUB_004139"
     CDS             <1..1115
                     /gene="yghO"
                     /locus_tag="ACHKUB_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO6657083.1"
                     /translation="EKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSAKNPG
                     TDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDDPQVFAALFGA
                     AEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQLGYHK
                     GIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNSGWQH
                     NWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADLNGSL
                     FPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFARRKID
                     ALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1122..1886)
                     /gene="glcC"
                     /locus_tag="ACHKUB_004140"
     CDS             complement(1122..1886)
                     /gene="glcC"
                     /locus_tag="ACHKUB_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO6657084.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2131..3630
                     /gene="glcD"
                     /locus_tag="ACHKUB_004141"
     CDS             2131..3630
                     /gene="glcD"
                     /locus_tag="ACHKUB_004141"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO6657085.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3630..4682
                     /gene="glcE"
                     /locus_tag="ACHKUB_004142"
     CDS             3630..4682
                     /gene="glcE"
                     /locus_tag="ACHKUB_004142"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO6657086.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4693..5916
                     /gene="glcF"
                     /locus_tag="ACHKUB_004143"
     CDS             4693..5916
                     /gene="glcF"
                     /locus_tag="ACHKUB_004143"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO6657087.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTLVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLERARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5921..6325
                     /locus_tag="ACHKUB_004144"
     CDS             5921..6325
                     /locus_tag="ACHKUB_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO6657088.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6347..8518
                     /gene="glcB"
                     /locus_tag="ACHKUB_004145"
     CDS             6347..8518
                     /gene="glcB"
                     /locus_tag="ACHKUB_004145"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO6657089.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPVQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8874..10556
                     /gene="glcA"
                     /locus_tag="ACHKUB_004146"
     CDS             8874..10556
                     /gene="glcA"
                     /locus_tag="ACHKUB_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO6657090.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2487 a         2704 c         3015 g         2503 t
ORIGIN      
        1 ttgaaaaagt aattaataaa aatgacctta aggcatttat cgcttttccg tcatcacttt
       61 atcccgacga tccaaactgg ataccccctt tattcataga acgcaacgag catttgtctg
      121 cgaaaaatcc ggggacggac catatcatct ggcaggcgtg ggtggcaaaa aaagcggggc
      181 agatagttgg gcgtattacc gcgcaaatag ataccttgca ccgtgagcgt tacggtgaag
      241 ataccggtca tttcggcatg attgacgcca ttgatgatcc gcaggttttt gccgcgttgt
      301 ttggcgcagc ggaagcgtgg ttgaagtcac aaggtgcaag taagatcagc ggtcctttca
      361 gcctgaatat caatcaggaa agcggattac tgattgaagg ttttgacaca ccaccctgtg
      421 cgatgatgcc acacggcaaa ccgtggtatg ccgcgcatat tgaacaactg ggttatcaca
      481 aaggcattga tttactggcg tggtggatgc agcgaaccga tctcactttc tctccggcgc
      541 taaaaaaact gatggatcag gtgcgcaaaa aggtgaccat tcgctgcatc aatcgtcagc
      601 ggtttgccga agaaatgcag atcctgcgtg agattttcaa ctctggctgg cagcacaact
      661 ggggatttgt gccgtttacc gaacatgaat tcgcgaccat gggcgatcaa cttaaatatc
      721 tggtgccgga cgatatgatc tatatcgctg agattgattc tgcaccctgc gcgtttattg
      781 tcggcttgcc gaacatcaac gaggcgattg ccgatctgaa cggatcgctc tttcccttcg
      841 gctgggcaaa attgctgtgg cgtttgaaag tcagcggtgt gcgaactgcg cgagtaccgc
      901 tgatgggcgt gcgcgacgag tatcagttca gccgcatcgg cccggtgatt gcgctgttat
      961 tgattgaagc cttacgcgat ccgtttgccc gccggaagat cgacgcgctg gagatgtcat
     1021 ggatcctcga aaccaataca ggcatgaata acatgctgga acgcattggg gcggagccgt
     1081 acaagcgtta ccgattgtat gaaaagcaga tttaatacca gctaactcag gttcatctcc
     1141 agcggcaccg ccgagcgaat caaatggtgg ccttccagct cgatatcgtg gagattcttt
     1201 ttcacggtcc gcacatgatc gcgtgctgcg cgctgggcga cgtgcggcag ccgctgcaac
     1261 accgcgttgt agatcctcgc atgctggcga tcgatctgct ttttttgctg tggtcgatgg
     1321 tagagattat ttacgctggc aaacactgaa ttaaacatca gatcggtcaa tgattgcagc
     1381 gtaaacacca gcacctgatt gtgagaagcc tgacaaatgg cgagatggaa agcgtgatcc
     1441 aactgcgcat gttcgatcag cgaaatctct ttgttgttct cactggcggc gagcattttt
     1501 tcataacagc gggttatcac aacaaaatca gcctgcgttc ccagcgttgc cgccagcctt
     1561 gccgattcgc cctccagtaa tgcgcgaacg tcgagcagat cgtacagcgt tcgcggctgc
     1621 gtactgaaca gatggatcag cgggctggtg tcctgcaccc gattaagccg tgcgacacga
     1681 gaatcgcgac cctgcgccgt ctcaataatc ccgcgcccgc gcagcacggt cagcccttca
     1741 cgcagtgcgg agcgtgagaa gccgagcttt tcacacagtc gacgttccga gggaagtggc
     1801 tgaccgacct tcagtacgcc gtcgataatt aaccgttcga tactctctgc aaccacttcg
     1861 caaatagggc gacgttcatc tttcattccc ggacctcgtg cacaggtagg accaattttt
     1921 ctgttcttaa ctcgcaaaac acgcacatca cgtaagtgat tatgttacat caatttaaca
     1981 ttgagttaac caaggcaagg tcacagagct ggaaaaaaaa tggtctgacc ggtagctaaa
     2041 gagatagacg aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa aaatcagctg
     2101 ccacacaaca caacaaagcg aagcctactc atgagcatct tgtacgaaga gcgtcttgat
     2161 ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga gcatgtccct
     2221 ggacttgaga tcctgcatac cgatgaggag atcattcctt acgagtgtga cgggttgagc
     2281 gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc aaatggaaca ggtgacagcg
     2341 attcttgctg tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc aggcaccggg
     2401 ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc gcgctttaaa
     2461 gagatcctcg acattaaccc cgttggtcgc cgcgcgcgcg tgcagccagg cgtgcgtaac
     2521 ctggcgatct cccaggccgt tgcaccgcat aatctctact acgcaccgga cccttcctca
     2581 caaatcgcct gttccattgg cggcaacgtg gcagaaaatg ccggcggcgt ccactgcctg
     2641 aaatatggtc tgaccgtaca taacctgctg aaaattgaag tgcaaacgct ggacggcgag
     2701 gcgctgacgc tgggatcgga cgcgctggat tcacctggtt ttgacctgct ggcgctgttc
     2761 accggatcgg aaggtatgct cggcgtgacc accgaagtga cggtaaaact gctgccgaag
     2821 ccgcccgtgg cgcgggttct gttagccagc tttgactcgg tagaaaaagc cggacttgca
     2881 gttggtgaca tcatcgccaa tggcattatc cccggcgggc tggagatgat ggataacctg
     2941 tcgatccgcg cggcggaaga ttttattcat gccggttatc ccgtcgacgc cgaagcaatt
     3001 ttgttatgcg agctggacgg cgtggagtct gacgtacagg aagactgcga gcgggttaac
     3061 gacatcttgt tgaaagcggg cgcgactgac gtccgtctgg cacaggacga agcagagcgc
     3121 gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg taggacgtat ctccccggat
     3181 tactactgca tggatggcac catcccgcgt cgcgccctgc ctggcgtact ggaaggcatt
     3241 gcccgtttat cgcagcaata tgatttacgt gttgccaacg tctttcatgc cggagatggc
     3301 aacatgcacc cgttaatcct tttcgatgcc aacgaacccg gtgaatttgc ccgcgcggaa
     3361 gagctgggtg ggaagatcct cgaactctgc gttgaagttg gcggcagcat cagtggggaa
     3421 catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag cgatgaaatc
     3481 acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa ccctgggaaa
     3541 aacattccca cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca tcacggtcat
     3601 ttacctttcc ctgaactgga gcgtttctga tgctacgcga gtgtgattac agccaggcgc
     3661 tgctggagca ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt cagggcagca
     3721 atagcaaagc ctttttaggt cgccctgtca ccgggcaaac gctggatgtg cgttgtcatc
     3781 gcggcattgt taattacgac ccgaccgagc tggtgataac cgcgcgtgcc ggaacgccgc
     3841 tggtggcgat tgaagcggcg ctggagagcg ccgggcaaat gcttccctgc gagccgccgc
     3901 attatggtga agaagccacc tggggtggga tggtcgcctg cgggctggcg gggccgcgtc
     3961 gcccgtggag cggttcggtc cgcgattttg tcctcggcac gcgcatcatt accggcgctg
     4021 gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac gatctctcac
     4081 ggttaatggc cggaagctac ggttgtcttg gcgtgctcac tgaaatctcg atgaaagtgt
     4141 taccgcgacc gcgcgcctcc ctgagcctgc gtcgggaaat cagcctgcaa gaagccatga
     4201 acgaaatcgc ccagtggcaa ctccagccgc tacccattag tggcttatgt tacttcgaca
     4261 atgcgttgtg gatccgcctt gagggcggcg aaggatcggt aaaagcggcg cgtgaactgc
     4321 tggggggcga agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa caactgccgt
     4381 tcttctcgtt accaggtacc ttatggcgca tttcattacc cagcgatgcg ccgatgatgg
     4441 atttacccgg cgagcaactg atcgactggg gcggagcgtt acgctggctg aaatcgacag
     4501 ccgaggacaa tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg acccgcttta
     4561 gtgccggaga tggtggcttt gccccgctat cggctccttt attccgctat caccagcagc
     4621 ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac gcggaacttt
     4681 gaggagcagg ctatgcaaac ccaattaact gaagagatgc ggcagaacgc gcgcgcgctg
     4741 gaagccgaca gcatcctgcg cgcctgtgtt cactgcggat tttgtaccgc aacctgccca
     4801 acctatcagc ttctgggcga tgaactggac gggccgcgcg ggcgcatcta tctgattaaa
     4861 caggtgctgg aaggcaacga agtcacgctt aaaacacagg agcatctcga tcgctgcctc
     4921 acttgccgca attgtgaaac cacctgtcct tctggtgtgc gctatcacaa tttgctggat
     4981 atcgggcgtg atattgtcga gcagaaagtg aaacgcccac tgccggagcg aatgctgcgc
     5041 gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac gctggtaggg
     5101 ctggtgctgc gaccgttttt accggaacag gtcagagcaa aactgcctgc tgaaacggtg
     5161 aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct ggaaggctgc
     5221 gcccagccta cgctttcgcc caacaccaac gcggcaactg cgcgagtgct ggatcgtctg
     5281 gggatcagcg tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga ctatcatctt
     5341 aatgcgcagg agaaagggct ggaacgggcg cgcaataata ttgatgcctg gtggcccgcg
     5401 attgaagcag gtgccgaggc aattttgcaa accgccagcg gttgcggcgc gtttgtcaaa
     5461 gagtacgggc agatgctgaa aaacgatgcg ttatatgccg ataaagcgcg tcaagtcagt
     5521 gaactggcgg tcgatttagt cgagcttctg cgcgaggaac cgctggaaaa actggcagtt
     5581 cgcggcgata aaaaactggc cttccactgc ccgtgtaccc tacaacatgc gcaaaagctg
     5641 aacggcgaag tggaaaaagt gttgcttcgt cttggattta ccttaacgga cgttcccgac
     5701 agccatctgt gctgcggttc agcgggaaca tatgcgttaa cgcatcccga tctggcacgc
     5761 cagctgcggg ataacaaaat gaatgcgctg gaaagcggca aaccggaaat gatcgtcacc
     5821 gccaacattg gttgccagac gcatctggcg agcgccggtc gtacctctgt gcgtcactgg
     5881 attgaaattg tagaacaagc ccttgaaaag gaataaaaaa atgaaaacta aagtcattct
     5941 tagccagcaa atggcgagtg caattattgc cgcaggtcag gaagaggcgc agaaaaataa
     6001 ctggtctgtt tccattgctg ttgccgatga cggcggtcat ctgctggcgt taagtcgcat
     6061 ggacgattgc gcgccgattg cggcttacat ctcccaggag aaagcgcgta ccgccgcgct
     6121 ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac aacggacgta ccgcgttcgt
     6181 gactgcgccg ttactgacgt cgctggaagg tggcgtaccg gttgttgttg atgggcaaat
     6241 tattggtgcc gtgggcgttt ctggtttaac cggagcacag gatgcgcagg tcgcgaaagc
     6301 ggcagcagcg gtgttggcga aataagcgaa aacgaggaga taaacaatga gtcaaaccat
     6361 aacccagggc cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg aagaagtttt
     6421 accgggaaca gggctggacg ctgcggcgtt ctggcgcaat tttgatgaaa tcgttcacga
     6481 tctggcacca gaaaatcgtc agttgctggc agaacgcgat cgcattcagg cggcgcttga
     6541 tgagtggcat cgcagcaatc cggggccggt aaaagataaa gcggcctata aatctttcct
     6601 gcgtgaactg ggctacctgg tgccgcaacc ggagcgcgtg acggtggaaa ccacgggcat
     6661 tgacagcgaa atcaccagtc aggcggggcc acagctggtg gttccggcaa tgaacgcccg
     6721 ctacgcgctg aacgcggcga acgctcgctg gggctcactg tacgatgcgt tatacggcag
     6781 cgacatcatc ccgcaggaag gggcgatggt cagcggctac gatccgcaac gcggtgagca
     6841 ggttatcgcc tgggttcggc gtttcctcga tgaatctcta ccgctggaaa atggcagcta
     6901 tcaggatgtg gtggcgttta aggtggtcga taaacaatta cgcatccagt tgaaaaatgg
     6961 taaagaaacc acgttacgta ccccggtgca gtttgtcggt taccgtggcg atgccgctgc
     7021 gctgacctgc attttgctga aaaataacgg cctgcatatt gaactgcaaa ttgatgccaa
     7081 cgggcggatt ggcaaagacg atccggcgca catcaacgat gttatcgtcg aagcggctat
     7141 cagtaccatt ctcgactgcg aagattcggt cgcggcggtt gatgcggaag ataaaatcct
     7201 gctgtaccgc aatctgctgg gcctgatgca ggggactctg caagagaaaa tggagaaaaa
     7261 cggtcgacaa atcgtgcgta aactgaatga cgatcgtcat tacaccgccg ccgatggctc
     7321 tgaaatttct ctgcacggac gctcgctgct gtttatccgc aacgtgggtc atttgatgac
     7381 cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg gaaggcattc ttgatggcgt
     7441 catgactggc gcgatcgccc tctatgattt aaaagtgcag aaaaactcgc gcactggcag
     7501 cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa gtggcgttcg ccaacaaact
     7561 gtttacccgc attgagacaa tgctcggtat ggcaccgaat accctgaaaa tgggcattat
     7621 ggatgaagaa cgtcggacct cgctgaactt gcgtagctgt atcgctcagg cgcgcaatcg
     7681 cgtggcgttc atcaataccg gtttcctcga ccgtaccggc gatgaaatgc attcggtgat
     7741 ggaagctggc ccgatgctgc gtaaaaatca gatgaaatcg acgccgtgga tcaaagccta
     7801 cgagcgtaat aacgtgcttt ccggtctgtt ctgtgggctg cgcggtaaag cgcaaattgg
     7861 taaaggcatg tgggcaatgc cggacctgat ggcagacatg tacagccaga agggcgacca
     7921 actgcgtgcc ggggcaaaca cagcctgggt tccgtcacca accgctgcta cgctccatgc
     7981 gctgcactac caccaaacca acgtacagag cgtgcaagcc aacattgccc agaccgagtt
     8041 caatgctgaa tttgaaccgc tgctggacga tctgctgact attccggtgg ctgaaaacgc
     8101 taactggtcg gtggaagaga tccaacaaga gctggataac aacgtgcagg ggattctggg
     8161 gtacgtggtg cgctgggtgg agcaggggat tggttgttca aaagttccgg atattcacaa
     8221 tgtggcgctg atggaagacc gcgcaacgct gcgtatctcc agccagcata tcgccaactg
     8281 gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga atatggcgaa
     8341 agtggttgat cagcaaaacg ctgacgatcc ggcttatcgt ccgatggcgg ggaatttcgc
     8401 taactcgtgt gcttttaaag ctgccagcga tttaatcttc ctcggcgtga aacagccaaa
     8461 cggctatacc gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa gtcattaata
     8521 gcgtcaaaaa aagccccgac aagttttatt gtcggggcta tgatagtaac tgcggtagta
     8581 catttatccc ggaaataacc ggagcgatta tcagggttta acaatattaa atacccgtag
     8641 cgcattacta gcgggcctat aacaacaata tggacatctt aacgatactc tgcgcctgcg
     8701 tgtgcagaac agaaaccctt acccgatata cttgttatgc cagaggtagc aattgcgtta
     8761 gcggcgtact gttgctgtaa agtataacgc ctatatttta cagttcagtc ctcttttgct
     8821 tattacaacc ttaagtatat caagcatata aagataataa gagactgaac aatatggtta
     8881 cctggaccca aatgtatatg ccgatgggag gactggggct atccgctctg gtcgccctga
     8941 tcccgataat attcttcttc gttgcactcg cggtattacg tctgaaagga catgtcgctg
     9001 gagcaataac ccttatatta tctatcctga ttgcaatatt cgcctttaaa atgccgattg
     9061 atatggcttt tgctgctgcg ggctatggct ttatttatgg attatggcct attgcgtgga
     9121 ttattgtcgc ggcggtgttc ctctataaat taaccgttgc cagcggtcag ttcgatatta
     9181 tccgcagctc ggttatctcc atcaccgacg atcagcgttt gcaggtgtta ctgattggtt
     9241 tctcctttgg tgcgttactg gaaggagcgg ctggctttgg tgcgccggtg gcgattaccg
     9301 gtgcgctgct ggtgggcctg ggcttcaaac cgttatacgc ggcggggctg tgtctgattg
     9361 ccaacactgc gccagtggcg tttggtgcgt tgggcgtgcc gattctggtc gccggtcagg
     9421 taacgggaat cgatccgttc cacattggcg caatggcggg acgtcagtta ccgttcctgt
     9481 cggttcttgt gccgttctgg ctggtagcaa tgatggacgg ctggaaaggg gtgaaagaga
     9541 cgtggccagc ggcgctggtt gctgggggaa gctttgctgt cactcagttc tttacctcta
     9601 actatattgg tccggaactg ccggatatta cttcggcgct ggtgagtatc gtctcactcg
     9661 ctttattcct taaagtctgg cggccgaaaa ataccgaaac ggcaatcagc atggggcaat
     9721 ccgcaggtgc gatggtggta aataagccat cttctggcgg tcccgtgcct tcagaatata
     9781 gtctggggca aatcattcgt gcgtggtcac cgtttttaat cttaacggtg ctggtcacca
     9841 tctggaccat gaagccgttt aaagcgttat ttgctccggg cggcgcgttt tattcactgg
     9901 tgattaattt ccagatccct catttgcatc aacaagtgtt gaaagcggca cccattgtcg
     9961 cccaaccaac gccaatggat gcggtgttta aattcgaccc tctctcggct ggcggcaccg
    10021 ctatttttat tgcggcgatt atctctatct tcatcctcgg cgtgggcatc aagaaaggga
    10081 ttggcgtctt tgccgaaacg ctaattagtc tgaagtggcc gatattgtcg attggcatgg
    10141 tgctggcgtt cgccttcgtc accaactatt ccggcatgtc caccacgttg gcgctggtac
    10201 tggcaggtac aggcgtgatg ttcccgtttt tctcaccgtt cctcggctgg ctgggggtat
    10261 tccttaccgg ctcggacact tcttcaaacg ccctgtttgg ttcactgcaa tcgaccacgg
    10321 cgcagcaaat caacgtctct gacactctgc tggtggcagc aaataccagc ggcggcgtaa
    10381 ctggcaagat gatctccccg caatctatcg ccgtggcctg cgccgcgacg ggcatggtgg
    10441 gccgagaatc tgaactattc cgctacaccg tgaagcacag tctgattttt gccagcgtta
    10501 tcggcgttat cacgttgttg caggcgtatg tatttactgg aatgttggtc tcgtaaatca
    10561 ataatgccgg atgatcgtta atatcatccg gcttcattta cattattctg tcttattaat
    10621 tgaaaattat ttctcgcttt attatccatg cggcaataat gttttgtgtt cagataacct
    10681 gctaaataat taagagcgtg agaacatct
//
LOCUS       DAZFRN010000162        10694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFRN010000162 DAZFRN010000000
VERSION     DAZFRN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.164"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            511..726
                     /locus_tag="ACHKUB_004147"
     CDS             511..726
                     /locus_tag="ACHKUB_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6657091.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAAREE"
     gene            731..1264
                     /locus_tag="ACHKUB_004148"
     CDS             731..1264
                     /locus_tag="ACHKUB_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6657092.1"
                     /translation="MSRKFRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPSMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFSSTFYRKLNAGDRKGACAEIRRWIYDGGQDCRIRSNNCYGQ
                     VSRRDQESALACWEIDR"
     gene            1535..2105
                     /locus_tag="ACHKUB_004149"
                     /pseudo
     CDS             1535..2105
                     /locus_tag="ACHKUB_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
     gene            2105..2251
                     /locus_tag="ACHKUB_004150"
     CDS             2105..2251
                     /locus_tag="ACHKUB_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657093.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFSLMLVALFLSMAWMALTSA
                     GLVG"
     gene            2474..2680
                     /locus_tag="ACHKUB_004151"
     CDS             2474..2680
                     /locus_tag="ACHKUB_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6657094.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            2745..2969
                     /locus_tag="ACHKUB_004152"
     CDS             2745..2969
                     /locus_tag="ACHKUB_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657095.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVEYTMNAAKCRMVSAALQPVC"
     gene            3315..3641
                     /locus_tag="ACHKUB_004153"
     CDS             3315..3641
                     /locus_tag="ACHKUB_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO6657096.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(3773..3973)
                     /locus_tag="ACHKUB_004154"
     CDS             complement(3773..3973)
                     /locus_tag="ACHKUB_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6657097.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            4015..4380
                     /locus_tag="ACHKUB_004155"
     CDS             4015..4380
                     /locus_tag="ACHKUB_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6657098.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            4670..5233
                     /locus_tag="ACHKUB_004156"
     CDS             4670..5233
                     /locus_tag="ACHKUB_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6657099.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            5230..6891
                     /locus_tag="ACHKUB_004157"
     CDS             5230..6891
                     /locus_tag="ACHKUB_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6657100.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQNGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLVEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            6955..8892
                     /locus_tag="ACHKUB_004158"
     CDS             6955..8892
                     /locus_tag="ACHKUB_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6657101.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            8937..9158
                     /locus_tag="ACHKUB_004159"
     CDS             8937..9158
                     /locus_tag="ACHKUB_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657102.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            9104..10694
                     /locus_tag="ACHKUB_004160"
                     /pseudo
     CDS             9104..10694
                     /locus_tag="ACHKUB_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT         2497 a         2514 c         3247 g         2436 t
ORIGIN      
        1 ccgttcacac tggtgaaatc tccggcatca ggggcggcgg acaaactgca actgacaggc
       61 atatcaaagg cggcgacata tacgctgctg attgacagtg tgaaggtgga agtgaacaac
      121 gcggacggcg cggcatgata aaaaaaccgc cagtcgcggg aatatcgctg gcggtaagta
      181 cccatgtacc tttgtaatga atggatgttt ttacatcggt tattttttaa tgaattgcgt
      241 ttctgttgtc aacataccag gcgggaagtt ttcaggacaa aaaatccacc agtaagcaag
      301 ggaggtgcag ttgcgtgctt ctggtggaaa tggtacggct atgaggtttt attgttatgc
      361 atgccataat ttttctacac aaaagcaatt ctgaatgtca acacgtttta taaggaattg
      421 ttgtgcacac agagtaaatg cacggggctg tcagtgtaac actggcggcc tttttcatgt
      481 tgtgagcttc cggattgcgg gagacggggt atgtaccaga tggaaaaaat cacaacaggt
      541 gtgtcataca ccacgtcagc ggtgggaacg ggctactggt tcctgcagtt gctggacagg
      601 gtttccccgt ctcagtgggc ggcaataggc gtgctgggga gtctgctgtt tggtctgctg
      661 acatatctga ctaacctgta tttcaaaatt aaagaagacc ggcgtaaggc ggcccgggaa
      721 gagtaagctg atgagcagaa aattccgcta tggtttatcg gctgccgttc tggcgctgat
      781 tgccgcaggg gcttctgcgc ctgaaatcct cgaccagttt ctggatgaaa aggaaggcaa
      841 ccacaccacg gcataccgtg atggtgcggg tatctggacc atctgccgag gtgccatcat
      901 ggtggatggt aagcctgtga ttcctagcat gaagctgtcg aaggaaaaat gcgaccaggt
      961 taacgccatt gagcgtgata aggcgctggc atgggtggag aaaaacatca aagtgccact
     1021 gactgaaccc cagaaagcgg gtattgcgtc attctgtcct tacaacattg gccccggtaa
     1081 gtgtttcagc tcaacgtttt accggaaact gaacgccgga gaccgtaaag gcgcatgtgc
     1141 tgaaattcgt cgctggatat atgacggtgg tcaggactgc cgtattcgtt caaacaactg
     1201 ttacggtcag gtatcccgtc gtgaccagga gagcgcgctg gcgtgctggg aaatcgacag
     1261 ataagcagaa tattttgctg aaaaatgagg tttgctcaca tggacgtata acacgaaatc
     1321 ctgcaaattg gcaaaatgta agtgaataaa gtcaaaacag ttgtttaaca ctcaggcacc
     1381 gtaatggtgc ctttgtcatt tctgcgcatc tcacgcgcat ctcacaacac agaacctttc
     1441 aggatgaccc ttgaggatac cggtttggct gtcggtgcct ttctgtgggc tggattcctg
     1501 tgagacaagg ttcatcacta aaaggaaata accgatgaat atgatgaccg tgccgtttca
     1561 cggcaactct ctttatgtag ttaaccataa tggcgagcca tacgttccca tgaaacctgt
     1621 cgttgcgggg atggggctgg cctggcaatc acagttggct aagttaagac agcgttttgc
     1681 gtcaactata acggaaatcg ttatggttgc tgaggatggg aaacaacgca atatggtgtc
     1741 catgccactt cgaaaacttg ccggctggct acaaactatt aatcccaaca aagtaaaacc
     1801 cgaaatccgc gataaggtca tccggtatca ggaagagtgc gacgatgttc tttacgagta
     1861 ctggacgaag ggttttgtcg ttaatccccg taaaatgagc gtgatggaag aactcaacca
     1921 ggcttgtgct gacatgaaac gggataaaaa acattgccag tgtgtttgct accgggctga
     1981 atgagtggaa acaggttaaa gccgcgcatg tatcaaaaat ccgtacgctg gtaaatgaag
     2041 cgaatatgct gattgatttt gtcctggctg atacaggcaa agggaaaata acaaaggcgg
     2101 attgatgggg tggctaatga tatcagataa actcataacg ctggtgaaga gcctctgtgt
     2161 acttgtcggc atttcatttt cactaatgct ggttgctctt tttctttcca tggcctggat
     2221 ggcgttgact tcggcagggc tggtggggtg agcataaacc gaatgctttc cgcgtttacc
     2281 gttattctgc tggtggtctg tggtgcgctt agtctggggc tgaatcatta ccgcgataac
     2341 gccataacct acaaagcgca gcgcgataaa aaagtcagtg agctggagct ggcaaatgca
     2401 accattactg atatgcagca gcgccagcgt gatgttgctg cacttgatgc cagatactcg
     2461 agggaattag ccgatgcgag agctgaaaat gaaactctgc gtgctgatgt tgccgctggt
     2521 cgtaagcgcc tgcggatcaa cgccacctgc cccggtaccg tgcgtgaagc caccggcacc
     2581 tccggcgtgg gcaatgatgc cgccgtcgaa ctctctccgg ttgctggacg aaacgttctc
     2641 ggtatcagag acggaatcat cagcgaccag gcagcattga gaatgcttca ggaatatatc
     2701 cgcactcagt gtattaacta gtatttttgt tattcggaga atgcatgaag aaattactgg
     2761 taaccgtaaa gccttttcag ggaacaattc cgttccgtat tttgcagcgt ggtcgtgttc
     2821 ttgttgaagg ttcgttcagt ggtaaatgta cgcaattaca ctcccggacc tttcaggtga
     2881 atgccacgaa tgaagagcta accgttgagt atacgatgaa tgccgctaaa tgccgcatgg
     2941 tatccgctgc attacagcca gtgtgttgag cgaccttatt atccatggcg cggtattgtc
     3001 gccgtattcc cgtattaaca gagaccgcag cccgacaggg agactcctct gcgcgagtgt
     3061 gcggggataa tcaaaaacga tacacaccgg ggtttaccgc gttaacggag cgcggcgttg
     3121 tcccctcatg gtcgctggtc cggtgcgatg gtggaagaaa ccggacgatg tgttacctcg
     3181 caagctctgt tatgtcatgt gtctgatttg tgatttaagt cggataattg tcgttgccat
     3241 taagcagagg attgatgacc gacagggtgg cattgttaga ataagactta ttcttatctg
     3301 tgccgggaat gaaaatgaaa agaaatcttc cgttaattat tttgttgtct tctctggtta
     3361 tgggctgtac gcaacataaa acagatatgc cccgacagtt ggttaaggca ttaccacaat
     3421 atccggccta tgcagcggca aattatataa agggacgggt tgatgtgaag tttgatattg
     3481 gtgctgatgg tactgtcacc cgaattgagt ttatccgttc agagccgcac catctgtttg
     3541 atgagcaggt tgtaaaggcg atggcaaaat ggcgatttga gaaggacagg ccgcgtaaag
     3601 gcgtgaagaa aacgtttatc tttagtcctt ctgcaccctg attatttcat cagaaattaa
     3661 ttatcactct gttgttattc tgtacatccc ggcagggtaa gtcttgttcc gtcggatatg
     3721 aagatgaaat attgttggag gacagtgggt agctgctcct gtaaccgaac gttcatttct
     3781 cgttatttgt catgctggcc gggcgcaggt gcgttgcatc tgttgccagc cttctccggc
     3841 aggcttcaat aacccacgct gaaaagttac cggacccttt atgctcaagg gcgatgttga
     3901 tctgttcaat catgtgattg gggaaacgga tattgcgggt tgtggttctg cgggtccggt
     3961 ttttcgatga catatttatt tcctttactg attgccatat gacggggatt ttacatggct
     4021 gagcttcgta cactccagag cagaatcaaa acactgaata cccgacgggt gaatattctg
     4081 aagggtgaac agcgtcgtgt cagtggcagt gcacgtgttt ccctcaagcg tcatatctgg
     4141 ctcagggatg ccgggcagtg ctgtctctgt ggtcgtgtgg ttgacctctg tgacagtgaa
     4201 ctcgatcacc gaattgcact tcagttcggt ggtggtaatg aggagacgaa tctctggacg
     4261 ctctgtaccg aatgccatcg acaaaagtct gctcgtgaag cggcgggtgg tatgccggac
     4321 ccgacgctgc cggaggtgtc cggaggtagt ggcagagcgg acgacatcat cggactgtaa
     4381 cccgaccggg gggggtatca ttcggcgtaa aaaacgatcg ctccggacac cgcgccccct
     4441 ctcacgcaga gaaaaaattc ccgtttcagg gcagttaaca tgttaactgg ctgtccgggc
     4501 atttttgcgg tttttatctt tattattcag tttgttgcgc ggaaaaaatg ttaactggct
     4561 ttttcagcaa atgttaacca ggcagcagtt aacatttgcg gcatgagacg ccgggaaaaa
     4621 tgggctgaac catacccggc tgagtgcgtt ctggacccgg gaggaggctg tgctgacaac
     4681 gcaaaaacga aaatttgcgc tggcgctcat gtccgggaaa aacaaaacag cgtcagccat
     4741 tgccgccggt tattcggcga agacagccag ggttaaaggc tcgcagctgg caaaagatcc
     4801 ggaggtgctt gcgtttatag cccgtaaaca gtgcgagacg gtggaggtgg atgaggttcc
     4861 tgtttaccgg cagaaaaaat cagagcagga ggataaaccc cgtcgccgtg aggcggctgc
     4921 aataccacag ccggacgaaa caaatccgga gatgccaccg cccgtggtga tatctcctgg
     4981 tattgagtat atggaggacg gtcttcccga tccggtgaaa gcgatggggc gtcttctggt
     5041 ggagaacatt aataccgacc ccaggctggc gctggatgcg gcttataagc tggcgcagtt
     5101 cacgcaccac aaaaaagggg atgccggtaa aaaatcggca aaaggtgacg cggcgaaaaa
     5161 agcagctaac cgttttgcgg tgccaccacc accccgactg gtggtgaata atgataatga
     5221 gggcaacgga tgatacctgt gtggagcacg gcctgcccgg actgggcaga gcgcctgaaa
     5281 aaggggctgt cgattattcc ggctccgatt tatccggagc aggccgcaca tgccctggcg
     5341 atttttaaac aactgcggat tgtggatgca ccgggcagcc cgacgttcgg ggagtcctgt
     5401 gcaccgtggg tgtttgacct ggtggcggcc ctgtttggct cctacgatgc gcagaacggt
     5461 gtacgccata tcaaggaagt ttttatcctt atcccgaaaa aaaacagcaa gtccacgctg
     5521 gctgccggga tcatgatgac ggcgctgtta ctgaactggc ggcaggcggc gggctacacc
     5581 attctggccc cgaccgtgga ggtggcggct aacgccttta atcctgcgcg ggatatggtc
     5641 agacgtgacg atgatctgga tgacctctgc caggtacaga cacatatccg gaccatcacc
     5701 cacagggtga cggacaccac ccttaaggtg gtggcagccg atccgaatac ggtgtccggt
     5761 atcaagtctg tggggacact gattgatgaa ctgtggctgt ttggcaagca gtacaaagcg
     5821 gaggacatgt tacgtgaagc catcggcggg cttgcctccc gcccggaagg gtttgtggtg
     5881 tatacgacca cccagtcgaa tgaaccgccc gccggggtgt tcagacagaa actgcagtac
     5941 gcccgggatg tgcgcgacgg caaaattcat gatccgcact ttctgccggt gatatttgag
     6001 catccgccgg agatggtgga gcgcggtgag cacctgctga tggaaaacct cgccatggtt
     6061 aacccgaatc tcggttattc ggtggatgag gcttttctgt accgggagta ccgtaaagcc
     6121 cgggaggctg gtgaggaagc atttcgtggc ttcatgtcaa aacacgccaa tgtggaaatt
     6181 ggtcttgccc tgcgttctga ccgctgggcg ggtgcggatt tctgggagca gcagggcagg
     6241 cgcgtcagcc tggacgatat cctgcagcgc gctgatgtgg tgacggtggg gattgacggc
     6301 gggggcctgg atgatctgct gggaatgtac gtgattggtc gtgacaggga aacccgcgaa
     6361 tggctgggct ggggccatgc ctgggcgcat gaaaccgcgg tggtcagacg gaagagtgag
     6421 gcatcccgtt ttcaggattt tgtggcctgt ggagacatga cgattgtccg tcgggtcggg
     6481 gatgacacgg cggaagtggc ggagtatgtg cgtcgtattc atgaggctga gttactggat
     6541 catatcggta ttgacccgtc aggtgtgggg cagattctgg attcactggt ggaagccggg
     6601 atccccgacg gaattgttgt ggggataagc cagggctgga agctgggcgg ggccataaaa
     6661 accaccgagc gcaaactggc tgagggagta ctggtgcatg gtggtcagcc actgatggcc
     6721 tggtgcgttg gcaatgcccg ggtggagccg aaaggtaacg ccatccttat taccaaacag
     6781 gccagcgggc gggggaaaat tgacccgctg atggcgctct tcaatgcggt atccctgatg
     6841 tccctgaatc cggagccgaa aaagaaagaa tatgcggttt ttttcatata accctgttca
     6901 ccctgtaacc atcatggacc gctgcggcgg tttttttatt ttcaggaggc tgatgtgact
     6961 cttaaacggg cctgctccct gctgacggtg aaatccttca gtgaggatga gcgggtgatc
     7021 accgggattg cgtcaacgcc ttctccggat cgggatggtg acatcctgga gccggagggc
     7081 gcggagtttg gcagtgcgat cccgtttctc tggcagcatg accattcccg cccggtgggg
     7141 cagtgtacgg tgcgccgggt cagcgaaggg ctggaaatca cggcaacact ggcgaagccc
     7201 gtgccggata tgccatcgca actggctgcc aggctggatg aggcctgggc ggccattaag
     7261 accgggctgg tcagggggct gtccgtgggc ttccgtcctc atgaatacac ctttctggac
     7321 ggaggcggac tgcattttct gcgctgggag ctgatggagg tgtctgccgt caccgtgccc
     7381 gcgaatgcgg aatgcaccat ccggaccatt aaatcttacg accgccagtt ttctgccgcg
     7441 tccggcaacc ggaaaccggt ggtgaaaatc gcatcttctg ccggcgctgc ggcacagtca
     7501 acaaccgttt ttcataagga aaagaccata atgaatattg gcgaacagat taaaagtttt
     7561 gaaaacaagc gtgcagcact ggcagcctcc cttgaggagg tcatgaccaa agccgcagag
     7621 gaagggcgca cgctggatgt ggaggaggaa gagcattacg acaacaccgc agcggaaata
     7681 cgtcaggtgg atgcgcacct gaagcgcctg cgtgaactgg aagccggtaa ggccgccacg
     7741 gcgcagccgg tgaaacaggc cggtaacggg aatgtggccg cggtggcttc tgcgccggtg
     7801 atccgtgtgg agcagaaact ggataagggg attggtttcg cacgttttgc caaatcgctg
     7861 gctgcggcta aaggtgtccg ctctgaagcc ctggaagtgg cccgtcgtca gtatccggat
     7921 gacagtcgtc tgcatcatgt cctgaaatcg gcagtgggcg cggggaccac cacggatccg
     7981 cagtgggcag gcagcctgtc tgaatatcag gaatacgcac aggactttat tgattacctg
     8041 cgtcctcaga ccattatcgg gcgatttggt cagggcggga tccctgcact tcgtcaggtg
     8101 ccgttcaata tccgtgtgca cgcccaggtg tcgggcggtg ctgccggctg ggtgggggag
     8161 ggtaaggcaa aacccctgac gaagtttgat tttgaatcca tcaccttcag tcatgcgaag
     8221 gtgtcggcca ttgcggtact gacggaagaa ttgatccgtt tttccagtcc ggctgctgat
     8281 gcactggtcc gtaatgcgct ggcggaagcg gtggtggcgc gtctggatac agactttgtg
     8341 gacccgaaaa aagcggcggt ggcagatgtc tccccggcgt ccatcaccca tgatgtgaag
     8401 ggcacggcat caagcggtaa cccggatgcg gatgcagagg cggcgtttgg acagtttgtg
     8461 gcagcaaacc tgcagcccac cggtgcggtc tggctgatgt ccagcaccaa tgctctggcg
     8521 ctgtccatgc gtaaaaatgc gctgggtcag aaagaatacc cggacatgac cctgctgggt
     8581 ggctccttcc aggggctgcc ggtgattgtc tcccagtacg tgggtgatca gctggtgctg
     8641 gtgaatgccc cggatattta tctggcggat gacggcggcg tggcagtgga tatgtcccgc
     8701 gaggcatcac tggaaatgca gtctgagccg accggcgaca gtaccacgcc gtcgccggtg
     8761 gagctggttt ccatgttcca gacaggcagc gtggccatcc gtgcggagcg ctggatcaac
     8821 tggcgtcgtc gccgtaccgc ggcggtggcg gtgatcaccg gagtgaacta tggcagtgcg
     8881 tccggcggct gagtctgata aggaggacgg gaggcgtgtg cctcccgtaa caagttatgg
     8941 caaagatccg atatctgcag ggcacgcatg atgcccgggc cggggatatc cgtgatgtgg
     9001 cacagccgtg tgcggaggtg ctggttcgcc tgggaaaggc ggagtacatc acggtgcgac
     9061 gtccggcagg tcagaaaaag aaacgtgatg cggagcatgg cgaatgtgga accttttacg
     9121 gcgaacccga aaaaaccaga aatcaggacg tgacgtaaga gaggcgggct ggaccagcct
     9181 gtttcaggcg gtggctgagc ccttttccgg cgcctggcag cagggcgtga aagccgatcc
     9241 tgaagccgtc ctctcctttc atgcggtgtt tgcatgtatt tcgctgatat cccaggatat
     9301 cgccaaaatg cggctgcgtc ttatgcagac ggatgcgcag gggatacgca gggaaacgcg
     9361 ccggggggat attgcccgcc tctgtcgtcg tcccaacgcc cagcagaacc gcatccagtt
     9421 ttttgaactg tggctgaacg ccaaactgcg tcacggcaat acggtggtgc tgaaaatccg
     9481 taatgcccgg gggcagatca aagaactgcg tattctggac tggaaccggg ttgaacctct
     9541 ggtggcggat gacggcgagg tgttctaccg catcacgccg gaccggaact gcgggatcac
     9601 tgaggcggtg acggtgcctg cccgggaagt gatccacgac cggtttaact gtttttttca
     9661 tccgcttata gggttgccgc cggtgtatgc cgccgggctg gcggccacgc aggggcatca
     9721 tattcaggaa aattcgacgt cttttttcag aaatggcggc aggccgtccg gggtgattga
     9781 gatccccggc agtattacgg aagaaaatgc gaaaaaactg aagagcaact gggacagcgg
     9841 gtatacaggc gaaaatgcgg ggaaaacggc cattctgagc aacggggcaa aatacaaccc
     9901 cacgacgttt tcaccggtgg attcgcagac ggtggaacaa ctgaagatga ccgctgaaat
     9961 tgtctgttcg gtgttccgtg tcccggccta caagattggc gtgggacaac cgccttccag
    10021 tgacaacgtg gaggcgctgg agcagcagta ttattctcag tgcctgcaga cgctgattga
    10081 gtccattgaa ctgttactgg atgaggcgct ggaaacgggg gaaaacgaga gtacggaatt
    10141 tgatgtcacc acgctgctga gaatggacag tgagcggcgc atgaaaacgc tgggggatgc
    10201 ggtgaaaaat acgcttctca cgcccaatga ggcccgtaaa cgggagaacc tgccgcccct
    10261 ggccggcggt gatgcactgt atcttcagca gcagaactat agtctggaag cgttgtcccg
    10321 ccgtgatgcc cgtgaagatc cgttcgcgtc gtccggtaaa acagcttccg tgcctcaggc
    10381 ggtcgttgcc tctgacggta ataaggcaat cactgaaaca gagcatgatg cggtgaaggc
    10441 gatgttcagg gggattctga aaaaatgaat gaacgtgaac tgtccattat ccgtgcgctg
    10501 ggtgaagaat tttccgcagt gctggcggat ttaaagcgca catttgaggg gaaaatagcc
    10561 gcgcaggcac aaacgtttga agaaaaactg gcttccctgt ctgtggtatt acagaagtgc
    10621 gtgacgggcg atgatgtgcg tccgatgctt gagcagatgg tgaaggaggc ggtaagccat
    10681 atccctgttc cgcg
//
LOCUS       DAZFRN010000163        10446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFRN010000163 DAZFRN010000000
VERSION     DAZFRN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.165"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            126..701
                     /gene="ssuE"
                     /locus_tag="ACHKUB_004161"
     CDS             126..701
                     /gene="ssuE"
                     /locus_tag="ACHKUB_004161"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO6657103.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            694..1653
                     /gene="ssuA"
                     /locus_tag="ACHKUB_004162"
     CDS             694..1653
                     /gene="ssuA"
                     /locus_tag="ACHKUB_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO6657104.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1650..2795
                     /gene="ssuD"
                     /locus_tag="ACHKUB_004163"
     CDS             1650..2795
                     /gene="ssuD"
                     /locus_tag="ACHKUB_004163"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO6657105.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2806..3597
                     /gene="ssuC"
                     /locus_tag="ACHKUB_004164"
     CDS             2806..3597
                     /gene="ssuC"
                     /locus_tag="ACHKUB_004164"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO6657106.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEIARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3594..4361
                     /gene="ssuB"
                     /locus_tag="ACHKUB_004165"
     CDS             3594..4361
                     /gene="ssuB"
                     /locus_tag="ACHKUB_004165"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6657107.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4568..7180)
                     /gene="pepN"
                     /locus_tag="ACHKUB_004166"
     CDS             complement(4568..7180)
                     /gene="pepN"
                     /locus_tag="ACHKUB_004166"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO6657108.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7446..8648
                     /gene="pncB"
                     /locus_tag="ACHKUB_004167"
     CDS             7446..8648
                     /gene="pncB"
                     /locus_tag="ACHKUB_004167"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO6657109.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8817..10217
                     /gene="asnS"
                     /locus_tag="ACHKUB_004168"
     CDS             8817..10217
                     /gene="asnS"
                     /locus_tag="ACHKUB_004168"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO6657110.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2367 a         2699 c         2800 g         2580 t
ORIGIN      
        1 ctttagttta ttttcagaaa aagatacaca aaatttatat ttggcatttt cttgtctctc
       61 cgacagactg accgaattgt tatcaatgtt aacaaaaaaa gaacaattgg ttataaggag
      121 agagtatgcg tgtcatcacc ctggcgggta gtcctcgctt tccttctcgc tccagctcct
      181 tgctggaata tgcgcgggaa aaactaaatg gcctcgatgt agaggtttat cactggaatc
      241 tgcaaaactt cgccccggaa gatctgcttt atgctcgttt cgatagtccg gcactcaaga
      301 ccttcaccga acagctgcaa caggctgatg gactgatagt cgctacacct gtgtataaag
      361 ccgcctattc cggcgcgctg aaaaccctgc tcgacctgct gccagagcgc gctttgcaag
      421 gcaaagtggt gctaccgctg gcgacgggcg gtaccgtggc ccatctgctg gcggtcgatt
      481 atgcccttaa accagtttta agcgcactga aagctcagga gatcctgcac ggcgtgtttg
      541 ccgatgactc acaagtaatt gattaccatc acagacccca gttcacgcca aatctgcaaa
      601 cccgtcttga taccgcgcta gaaactttct ggcaggcatt gcaccgccgc gatgttcagg
      661 ttcctgacct tctgtctctg cgaggtaatg cccatgcgta acatcattaa actggcgctg
      721 gtgggattgc ttagcgtctc tacgattgcg gttgctgcag aatcctcgcc tgaagcgtta
      781 cgtataggct atcagaaagg cagtattggt atggtactgg caaaaagcca ccagttactg
      841 gaaaaacgct atccgcaatc aaaaatctcc tgggtggagt tccccgcggg tccgcaaatg
      901 ttggaagcgt taaacgttgg cagtattgat ctcggcagta ccggggatat tccgccaatc
      961 tttgcccagg ctgccggggc tgatttggta tatgtgggcg tcgagccacc gaagcccaaa
     1021 gccgaagtga ttctggtggc agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggt
     1081 cacaaagttg cctttcagaa aggttccagt tcacacaacc ttttactgcg tgcactgcgt
     1141 caggccggac ttaagtttac cgatatccaa cccacttacc tgacgcccgc tgatgcccgc
     1201 gccgcgttcc agcaaggtaa cgttgacgcc tgggctatct gggatcccta ctactctgct
     1261 gcattattac agggcggcgt gcgggtgctg aaagacggca ccgatctcaa tcaaactgga
     1321 tcgttttatc tggcagctcg tccctatgca gaaaaaaacg gcgcttttat tcagggcgta
     1381 ctcgcaacct ttagtgaggc cgatgcgtta acccgcagcc agcgcgagca aagcatcgct
     1441 ttactggcaa aaacgatggg cttaccggca ccggtgattg cctcttactt agatcatcgc
     1501 cctcctacca ccatcaaacc ggttaacgcc gaggttgccg ccttacagca gcaaacggca
     1561 gatctgtttt atgaaaatcg tctggtgccg aaaaaagtcg atattcgcca gcgcatctgg
     1621 cagcccactc aactggaagg aaaacaatta tgagtctgaa tatgttctgg tttttaccga
     1681 cccatggtga cgggcattat cttggaacgg aagaaggttc acgcccggtt gatcacggct
     1741 atctgcaaca aattgcgcaa gcggcggatc gtctgggcta taccggtgtg ctgatcccaa
     1801 cggggcgatc gtgtgaagat gcgtggctgg tggcggcgtc gatgatccca gtgacgcagc
     1861 ggctgaagtt tcttgtcgcc ctgcgcccca gcgtaacctc acctaccgtt gccgctcgcc
     1921 aggccgccac gcttgaccgt ctctcaaatg gtcgtgcgtt gtttaatctg gtcacaggca
     1981 gcgatccaca agagctggca ggcgacggcg tgttccttga tcatagcgag cgctacgaag
     2041 cctcggcgga atttacccag gtctggcggc gcttattgct gggggaaacc gtcaatttca
     2101 acggtaaaca tattcatgtg cgcggagcaa aactgctctt cccgccgatt caacagcctt
     2161 atcctccgct ttactttggc ggatcgtcag acgtcgccca ggagctggcg gcagaacaag
     2221 ttgatctcta cctcacctgg ggcgaaccgc cagaactagt aaaagagaag atcgaacaag
     2281 tgcgggcgaa agctgccgcg catggacgca aaattcgttt cggtattcgt ctgcatgtga
     2341 ttgttcgtga aactaacgac gaagcgtggc aggccgccga gcggttaatc tcgcatcttg
     2401 atgatgaaac tatcgccaaa gcacaggccg cattcgcccg gacggattcc gtaggacaac
     2461 agcgaatggc ggcgttacat aacggcaagc gcgacaatct ggagatcagc cccaatttat
     2521 gggcgggcgt tggcttagtg cgcggcggtg ccggaacggc gctggtgggc gatggtccta
     2581 cggtcgctgc gcgaatcaac gaatacgccg cgcttggcat cgacagcttt gtgctttcgg
     2641 gctatccgca tctggaagaa gcgtatcggg tcggcgagtt gctgttccca catctggatg
     2701 tcgccattcc ggaaattccc cagccgcagc cgctgaatcc gcaaggcgaa gcggtggcga
     2761 atgattttat cccccgtaaa gtcgcgcaaa gctaaggaag aaataatggc aacgccagtg
     2821 aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt ggcggtgtgg
     2881 caactggcct cctcagttgg ctggctgtcg acgcgtattt tgccctcacc ggaaggggtg
     2941 gtgacggcgt tctggacgct ctccgccagc ggcgaactgt ggcagcatct ggcgatcagt
     3001 tcctggcggg cgctgattgg cttttcaatt ggcggatcgc tggggttgat tttgggactg
     3061 attagcgggc tatctcgctg gggagaacgg ctgctggata cctcaattca gatgttgcgc
     3121 aacgtaccgc atctggcgct gatcccgctg gtgattttgt ggtttggcat tgatgaatcc
     3181 gcaaaaatct ttctggtggc gctcggcacg ctattcccca tttatatcaa cacctggcat
     3241 ggcatccgta atatcgatcg cggtctggtg gaaatagcgc gtagctatgg attatccggc
     3301 ataccgctgt ttatccatgt gatcctgcct ggtgccctgc cctcaattat ggtcggcgtg
     3361 cgttttgcgt tgggcctgat gtggctgacg ctgattgttg ccgaaaccat ttctgccaat
     3421 tcaggcattg gttatctggc gatgaatgcg cgggagtttt tgcaaacgga cgtggtggta
     3481 gtcgctatta ttctttacgc cctgctcggc aaacttgctg acgtcagcgc gcagctgtta
     3541 gagcgcctct ggttgcgctg gaacccggct tatcatttga aggaggccac ggtatgaata
     3601 ctgctcgtct gaaccagggc acaccattgt tgctcaatgc agtaagcaaa cattacgcgg
     3661 aaaatatcgt cctgaaccaa ctggatttac atattccggc aggtcagttt gtggcggtgg
     3721 tgggccgcag cggtggtggc aaaagtaccc tgctgcgcct gctggccggg ctggaaacgc
     3781 caaccgcagg cgatgtgttg gcaggcacca caccgctggc tgaaattcag gaagacacgc
     3841 gaatgatgtt tcaggatgcg cgtctgctac cgtggaaatc ggtgattgat aacgttgggt
     3901 taggccttaa aggccagtgg cgcgatgccg cgcgtcgggc gttggctgcg gtaggactgg
     3961 agaatcgcgc cggggaatgg cctgccgcac tttcaggcgg gcagaaacag cgagtggcgc
     4021 tggcaagagc gttgattcat cgaccgggtt tattgttgct tgatgaaccg ctcggggcac
     4081 tggacgcctt aacgcgactc gagatgcagg atttgattgt gtcactttgg caggagcacg
     4141 gctttaccgt actgttggtg acgcatgatg tgagcgaagc ggtagcaatg gctgaccggg
     4201 tgttgttaat tgaagaggga aaaattggtc tggatttgac agtggatatc ccccgtccgc
     4261 gtcgattagg gtcggtgcga ttagcggagc tggaagcgga agtattgcaa cgggtaatgc
     4321 ggcgtgggca ttctgaacaa ctgattcggc gtcatggtta atatgccgga tgtggcgcac
     4381 aaaaccagca ttttcaacgg actagtaggc ctgataagac acgtcaagca tcgcatcagg
     4441 cagttgggca cggctgccgg atgcggcgtg aacgccttat ccagcctaca aaaccagcaa
     4501 tttcaacgga gttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc attttggctc
     4561 aaaagtatca agccagtgct ttggtgatct tctcgtacag atcgccagag agattttcca
     4621 gccctttcaa ctgttccagc gccgcgcgca ttttctcctg acgtttggca tcataacgtt
     4681 tcaggcgaat cagcggttca atcagacgtg aagctacctg cgggttacgg ctgttgaggt
     4741 cggtaagcat ttccaccagg aactggtaac cgctgccatc ttcggcatgg aacgctgccg
     4801 ggttgctgcc cgcaaacgca ccaatcaacg aacgaatacg gttcgggttg ctcatggtaa
     4861 atgagcgatg ctgcaacaga ccacgcaccg tctccagcac attcgccgcc gggctggtgg
     4921 cttgcaggat aaaccattta tccatcacca gaccgtcctg atgccactta tcgtcgtact
     4981 cctgcatcag cgcgtcacgg caaggcagct gtgcagcaac cgccgcagaa agcgctgcca
     5041 gcgcatcggt catattgttt gcttcatggt attgcttgct caccagcacg tcagccagat
     5101 gcgtttcacc aaaagcaagg aagcgcaggc aggcattacg cagagtgcgt tttgcaatat
     5161 cttcatgctc aacacggtac tcgctctggt aattcgcgtt gtaaatagcc agcagctcat
     5221 ccgccagttc agtcgccaga gtacgagtga gtgcttcgcg tacttcggca atagcaatcg
     5281 ggtcgatgat atcgaacagt tcagccattt cattgacaga aggcagcgtc aggatttctg
     5341 ccgccagcgc cggatcaatc ttctcatcga gcagcaccgc gcggaaagcg tcagccacat
     5401 gtaccggcag agacagcggc tgcccttgct gatgacgggc gacgttcagc ttgatgtagg
     5461 ttgccagcaa actttgcgcc gcatcccaac gggagaaatc attacgcgca tgacgcatca
     5521 ggaaggtcag ttgctgatcg ctccacttat attccagttt cactggcgca gagaattcgc
     5581 acagcagcgc aggcaccggc tggaagtaga cattatcaaa aacaaaggtc tgttccgcct
     5641 gggtgacgtt cagcacggaa ttcaccggat gaccgccttt ctgcaacggg atcactttgc
     5701 cttcgttgtc atacaattcg atggcaaatg gaatatgcag cggctgtttt tctgcctgat
     5761 ccggcgtggc tggcgtgcgc tggctgatgg tcagggtgta ctgctcggtt tccggattgt
     5821 agtcgtcttt gacggtcaca atcggcgtgc cggactggct gtaccaacgg cggaaatggg
     5881 agagatcgac attcgacgca tcttccatcg cctgcacaaa gtcgtcgcag gttgccgcgc
     5941 tgccatcatg gcgctcgaaa tagagttgca tccctttctg gaagttttct tcgcccagca
     6001 gggtgtggat catgcgaatc acttccgcgc ccttctcgta aacggtcagg gtgtagaagt
     6061 tgttcatctc aatgaccata tccgggcgga tcgggtgcgc catcgggctg gcgtcttccg
     6121 caaactgcaa tccgcgcatg gtgcgcacat tattgatacg gtttaccgcg cgggaaccaa
     6181 ggtcagagct gaattcctga tcgcggaaga cggttaaacc ttctttcagg ctgagctgga
     6241 accagtcgcg gcaggtcact cggttaccgg tccagttatg gaaatattca tggccgataa
     6301 cgcgttcaat atcgagataa tctttgtcgg tggcggtgtc ggtgcgggcc aacacatatt
     6361 tggagttaaa gatattcagc cccttattct ccattgcgcc catattgaag aaatccaccg
     6421 cgacgatcat atagatgtcg aggtcatact ccaggccaaa gcgttcttca tcccatttca
     6481 tggagttttt cagcgaggtc atcgcccacg gcgcgcgatc aaggttgccg cgatcgacgt
     6541 acagctccag tgctacttcg cgaccagaac gcgtggtaaa ggtatcgcgc agtacatcaa
     6601 agtcgcctgc caccagcgca aacaggtagc acggtttcgg gaacgggtcc tgccactgta
     6661 cccaatggcg tccgttttcg agttcgcctt gcgcaacgcg gttaccattg gaaagcagga
     6721 agggatattt gattttatcg gcaataattt tggtggtaaa acgcgccagc acgtccgggc
     6781 ggtcgagata atacgtaata tggcggaaac cttcggcttc acactgggtg caaagcgcat
     6841 cgcctgactg ataaagccct tccagcgccg tattcgccgc cgggcttatt tcattaatga
     6901 tcttaagcgt aaaacgctcc ggcaaattac tgatgaccag tgcgccctct tcttctttcc
     6961 aggcggtcca cggctcatca ttaatatgaa cagaaaccag tttgaggtct tcgccgttga
     7021 gacgaagcgg agcatctgat gcaccatgac ggacagcctg gctgaccgcg gtaacgaccg
     7081 tcttttgcgc gtcgaggtca aaggtcaagt caatatcagt aatctggtaa tccggcgcac
     7141 gataatcgtg acggtatttg gcttgtggct gttgagtcat aaataacctt tagcatcttt
     7201 tatagagtct ggtgttcagt ctattcctgt tgcgtaaatc gcgctatgca gaatcttcat
     7261 cttttcaggt acaaacgcct ttattgctac atttttataa catacaccgc gtaatgccat
     7321 cgaccagaaa ggtggcatat ggtgtgatcg gggttcaata aattgcgaaa caaggtatac
     7381 tccagcagtt cctgaagatg tttattgtac taaacgctcc tgtacgagga cgctactgcg
     7441 cacctatgac acaattcgct tctcctgttc tgcactcgtt gctggataca gatgcttata
     7501 agttgcatat gcagcaagcc gtgtttcatc actattacga tgtgcatgtc gcggcggagt
     7561 ttcgttgccg aggtgacgat ctgctgggta tttatgccga tgctattcgt gaacagattc
     7621 aggcgatgca gcacctgcgc ctgcaggatg atgaatatca gtggctctct gctctgcctt
     7681 tctttaaggc cgactatctt aactggttac gcgagttccg ctttaacccg gaacaagtca
     7741 ccgtatccaa cgataatggc aagctggata ttcgtttaag cggcccgtgg cgtgaagtca
     7801 tcctctggga agttcctttg ctggcggtta tcagtgaaat ggtacatcgc tatcgctcac
     7861 cgcaggccga cgttgcgcaa gccctcgaca cgctggaaag caaattagtc gacttctcgg
     7921 cgttaaccgc cggccttgat atgtcgcgct tccatcttat ggattttggc acccgtcgcc
     7981 gtttttctcg cgaagtacaa gaaaccatcg ttaagcgtct gcaacaggaa tcctggttcg
     8041 tgggcaccag caactacgat ctggcgcgtc ggctttccct cacgccgatg ggaacacagg
     8101 cacacgaatg gttccaggca catcagcaaa tcagcccgga tctagccaac agccagcgag
     8161 ctgcacttgc tgcctggctg gaagaatatc ccgaccaact tggcattgca ttaaccgact
     8221 gcatcactat ggatgctttc ctgcgtgatt tcggtgtcga gttcgccagt cgctatcagg
     8281 gcctgcgtca tgactctggc gacccggttg aatggggtga aaaagccatt gcacattatg
     8341 aaaagctggg aattgatcca cagagtaaaa ctctggtttt ctctgacaat ctggatttac
     8401 gcaaagcggt tgagctatac cgccacttct cttcccgcgt gcaattaagt tttggtattg
     8461 ggactcgcct gacctgcgat atcccccagg taaaacccct gaatattgtg attaagttgg
     8521 ttgagtgtaa cggtaaaccg gtggcgaaac tttctgacag ccctggcaaa actatctgcc
     8581 acgataaagc gtttgttcgg gcgctgcgca aagcgttcga ccttccgcat attaaaaaag
     8641 ccagttaata tcatcaggga gctaatcggc tccctttttt tacctttaat tccgaaatct
     8701 ttcgctgcat ttgcgaattc tgcttgtctg attgcagatg ccaggtaaca taggtatccc
     8761 cccatttacg ggtggacaag tgtttatttt ttccgactat taacagagag aatattatga
     8821 gcgttgtgcc tgtagccgac gtactccagg gccgcgtagc cgttgacagc gaagtcaccg
     8881 tacgcggatg ggtacgtacc cgccgagatt caaaagctgg catctccttc ctcgccgttt
     8941 atgacggttc ctgctttgat cctgtacagg ctgtcatcaa taattctctg cccaattaca
     9001 atgaagacgt cctgcgtctg accaccggct gctcggtcat tgtgacgggt aaagtcgtgg
     9061 cgtcgccggg ccaggggcaa caatttgaaa ttcaggccag caaagttgaa gttgctggtt
     9121 gggttgaaga tccagacact tacccgatgg cggcaaaacg ccacagcatt gagtatctgc
     9181 gtgaagtcgc tcacctgcgt ccgcgcacaa acctgattgg tgccgtcgcg cgcgttcgcc
     9241 atacgctggc gcaggcgctg catcgcttct ttaacgagca gggattcttc tgggtttcaa
     9301 cgccactgat taccgcatct gataccgaag gtgcaggcga aatgttccgc gtttctacgc
     9361 tggatctgga aaacctgccg cgtaacgatc agggcaaagt ggatttcgac aaagacttct
     9421 ttggtaaaga gtctttcctg accgtatctg gccagttgaa cggcgaaacc tacgcttgcg
     9481 cattatccaa aatttatacc ttcggcccga ctttccgtgc tgaaaactcc aacaccagcc
     9541 gtcacctggc ggaattctgg atgctggagc cggaagtggc gtttgctaac ctgaacgata
     9601 tcgcgggtct ggctgaagcc atgctgaaat atgtcttcaa agcggttctc gaagaacgcg
     9661 cagacgatat gaaattcttc gctgaacgcg tagataaaga tgccgtttca cgtctggaac
     9721 gcttcattga ggccgatttc gcgcaggtgg attacaccga cgcagtgacc attctcgaaa
     9781 actgcggcag gaagtttgaa aacccggttt actggggcgt cgatctctct tctgagcatg
     9841 agcgttatct ggcggaagaa cactttaaag caccggtagt ggttaaaaac tatccgaaag
     9901 atattaaagc gttctatatg cgcctcaacg aagacggtaa aaccgttgcg gctatggacg
     9961 ttctggctcc gggcatcggt gagatcattg gtggctccca gcgtgaagaa cgtctggacg
    10021 ttctggacga gcgtatgctg gaaatgggcc tgaataaaga agattactgg tggtatcgcg
    10081 atctgcgtcg ctacggtact gttccgcatt caggtttcgg tcttggtttt gaacgcctga
    10141 ttgcttacgt aactggcgtg caaaacgtgc gtgatgtgat tccgttccca cgtactccgc
    10201 gcaacgccag cttctgattt tcttcctcaa agagccagcg cctgctggct cttttttatc
    10261 ctttctgtca agttatctgt ttgttaagtc aagcaatcta tttgcaaccc cgccataacg
    10321 atcatcctgt tacggaatat tacattgcaa catttacgca caaaaaataa cccgcattct
    10381 tattgcggat tagttttttc ttagctgata gcacaatttt catactattt tttggcattc
    10441 tggatg
//
LOCUS       DAZFRN010000164        10253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFRN010000164 DAZFRN010000000
VERSION     DAZFRN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.166"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            109..1791
                     /gene="ydeN"
                     /locus_tag="ACHKUB_004169"
     CDS             109..1791
                     /gene="ydeN"
                     /locus_tag="ACHKUB_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6657111.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1843..3000
                     /gene="ydeM"
                     /locus_tag="ACHKUB_004170"
     CDS             1843..3000
                     /gene="ydeM"
                     /locus_tag="ACHKUB_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6657112.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2966..3088)
                     /locus_tag="ACHKUB_004171"
     CDS             complement(2966..3088)
                     /locus_tag="ACHKUB_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657113.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3291..4976
                     /gene="yddA"
                     /locus_tag="ACHKUB_004172"
     CDS             3291..4976
                     /gene="yddA"
                     /locus_tag="ACHKUB_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO6657114.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHEHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDICTVL"
     gene            5014..7386
                     /gene="yddB"
                     /locus_tag="ACHKUB_004173"
     CDS             5014..7386
                     /gene="yddB"
                     /locus_tag="ACHKUB_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO6657115.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTSGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7443..10226
                     /gene="pqqL"
                     /locus_tag="ACHKUB_004174"
     CDS             7443..10226
                     /gene="pqqL"
                     /locus_tag="ACHKUB_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6657116.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQEAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3007 a         2271 c         2247 g         2728 t
ORIGIN      
        1 tttttagcta aatctgctat ctctttatgt gactaacttc acttacatcc acttatttct
       61 cttcgtaaaa ttacttcgga attaagtaca ataagaagag gaacatttat gaagtctgca
      121 ttaaagaaaa gtgtcgtaag tacctcgata tctttgatac tggcatctgg tatggctgca
      181 tttgctgctc atgcggcaga tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc
      241 tcagacttta cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc
      301 atggatgatc ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca
      361 atggaaaatc gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct
      421 gcacaaaaat caacgccgac gctcctttca ttaatggatg aaggcgtacg gtttactaac
      481 ggctatgtgg cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct
      541 cccgcccgct ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa
      601 actttcttgc ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg
      661 cacttgtcaa aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat
      721 gacaacttca ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac
      781 tttatgggat tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat
      841 cgtgaacgtg tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc
      901 gttgttgatc gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct
      961 ccgcacctgc caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt
     1021 agtcaaacag cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc
     1081 attctcgaac aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc
     1141 gataatggtg cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag
     1201 agtcagacct atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt
     1261 caacccggta attatgacaa gctgatttcc gcaatggatt tctacccgac agctcttgat
     1321 gcagccgata tcagcattcc aaaagacctt aagctggatg gcgtttcctt gctgccctgg
     1381 ttgcaagata agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct
     1441 cactggtttg acgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat
     1501 cagtcagacg attacccgca taaccccaac actgaggact taagccaatt ctcttatacg
     1561 gtgagaaata acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac
     1621 aaactgacgg atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa
     1681 gagatgcaag gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaagta
     1741 aatcaggaga agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt
     1801 catgcggcgg aattttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag
     1861 ccctccagtt ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg
     1921 cagtttactc atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat
     1981 atcgcagcgt ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct
     2041 ggcctggatt ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt
     2101 atttttaatg cattacaaac gaatggcatt ttattgaata atgaatggtg tgcttttctc
     2161 aaagaacatg agtttctggt tggtatctcg atcgatggcc cccaggagct acatgaccgt
     2221 tacagacgca gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt
     2281 ctgaaatcat atcaagttga gtttaatacg ttaaccgtca ttaataacgt taatgtccat
     2341 taccctcttg aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc
     2401 gaattgctcg aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc
     2461 cgtatcattg atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt
     2521 atgcaatggg ttaaaaacga tgtgggtgaa attttcatcc gtcagtttga aagctttgtc
     2581 agccgttttt tagggaatgg gcataccagt tgtattttcc aggagtcctg caaggataat
     2641 ctggttgttg aaagtaatgg agatatttat gaatgcgacc attttgtcta tccacagtac
     2701 aaaattggaa acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg
     2761 caaaaaaaac ggatttcagc gaaatgtctg aaatgtgcat ataaacctat ctgcaatggc
     2821 ggttgtccta agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa
     2881 ggttataaaa tccttttttc aaccatggta ccttatatga acgccatggt ggagttagct
     2941 aagaacagag taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa
     3001 taacgctctc ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga
     3061 cataaattcc tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac
     3121 gtggtatgca tcagggcatt agcaatatcc ttgattatca atagcataac cataaccact
     3181 gaaaatgaaa tattccttta tatttcatat tgttaaatga tacttaaata aatttcatca
     3241 gtaaatcatt gccatagata atcattggat ggcacaatca attttcgcta atgataacca
     3301 ttcccattac gttttgtatg ttgatagcaa agtatctctg tctgttgaag cccttttggc
     3361 tgcgtaagaa taataaaact tcagtgctgt taatcattat tatcctcgcc atgatccttg
     3421 gcgtggttaa aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa
     3481 gccagaaaga aaccgataag ctttggcagc ttgtcctttg gttccctgcc ctgctgggga
     3541 tctttgtatt aatatccgtg aacaaaacat ggttaattaa actgctgact attcgctggc
     3601 gcgaatggtt aacagattac taccttaatc gctggtttgc agataaaaac tactacttca
     3661 cgcagatcta cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca
     3721 ttctcttact aatcagcaaa acgctcagtc tttcatttgg cttcatccag tcacttagca
     3781 tgctgatcac ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg
     3841 gtggaacaga atggaatatc caggggtata tggtctatac cgttgtgctc attgtgatcg
     3901 gtggaacttt atttacccat aaggttggta agcgtattcg tccgcttaat gtggaaaaac
     3961 aacgtagcga agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga
     4021 ttgcgctaag caatgctgag tctctacaac gccaggagtt gagcgacaat tttcatacca
     4081 ttaaagagaa ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata
     4141 tttattcgcg ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg
     4201 ggcagatcaa tctgggcgga ctaatgaaat cgcgccaggc atttatgcta gtatcgaaca
     4261 atttaagctg gtttatttat aaatatgacg aacttgctga gctggctgcg gttatcgatc
     4321 gcttgtatga gttccatcaa ctcactgaac agcgccctac gaataagcct aaaaattgcc
     4381 aacatgcggt acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag
     4441 agaacttgaa ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg
     4501 cgggaaaaac cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata
     4561 tttcttctcc tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac
     4621 tgaaagagat tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag
     4681 tactgcatca ggttggtctg gggaaattgg ctgcgcgtat tcatgagcac gatcgctggg
     4741 gagatattct ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac
     4801 gtccgaaatg gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac
     4861 gcttactgcg tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc
     4921 aacccggtgt ctggaacctg gctgatgata tttgtgacat ttgcacggtt ttataacaac
     4981 aataaaagcc ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc
     5041 attttatgtg gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgcatttt
     5101 tttgaagaga tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac
     5161 accagtgaaa gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga
     5221 accaaccctg cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt
     5281 cgccctgaga aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac
     5341 ggtattagtg caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc
     5401 aatattagcg ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg
     5461 ctttatgaca gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca
     5521 aagatcaaac gcttcaacgc tgatgatagc aaggttaaat tgggttatcg cactacgcgt
     5581 tcggactggt taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggctct
     5641 tcaggaagta cttattactc cccagatttt aaaaagaact tttatacctt gtcgtttaat
     5701 caggaactcg ctgataactt cggcgttacc gccggtttat cgcgccgcca gtctgatatc
     5761 acccgcgcgg attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac
     5821 gttatcgata ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat
     5881 ttaaccttaa aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct
     5941 gatcgcgaaa tgggtaataa atcctacggt ctggcatggg atatggatac gcagctcgca
     6001 tgggccaaac tgcgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat
     6061 catgacatct ggtacaccga actttcatgt acatatggtg atattacagg gcgttgcacc
     6121 agtggcggat taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg
     6181 gactggcaaa aattcgccgt gggtaatgtt tcgcatcaac cctacttcgg cgcggaatac
     6241 atctattccg atgcgtggac tgaacgccat aaccagtctg aatcctatgt gattaatgct
     6301 gccggaaaga aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac
     6361 aactacacgc tgtatatggc ggatcgcatt agctggcgta atgtgtcatt aatgcccggc
     6421 gtgcggtatg actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg
     6481 gaatgggata tttttgctga tcaaacctca atgattacag caggttataa ccgttactat
     6541 ggcgggaata ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta
     6601 tcaggcaata aaaccctgac gcgttatcag gatttgaaaa cgccttataa cgatgaactg
     6661 gcaatgggat tgcagcagaa aatcggtaag aacgttattg cgcgcgcaaa ctatgtttac
     6721 cgtgaagcgc atgatcaaat cagcaaaagc agtcgtaccg acagcgcgac taaaaccacc
     6781 attactgaat ataacaacga cggcaaaaca aaaacgcatt cgttcaacct cagttttgaa
     6841 ctggccgaac ccctgcatat cagccaggta gatattaacc cgcaaattgt ctttagctat
     6901 atcaagagca agggcaactt gtcgttaaac aatggttatg aggagagcaa taccggtgat
     6961 aaccaggtgg tttataacgg taatctggtt tcttacgata gcgttccagt ggcagatttt
     7021 aataacccat taaagatctc cttaaacatg gatttcacgc atcaaccgag cgggttagtg
     7081 tgggcgaata cgctggcctg gcaagaagcg cgtaaagctc gcattatcct gggtaagaca
     7141 aatgcgcaat acatcagcga atattcagat tacaagcagt atgttgacga aaaactggat
     7201 agcagcctga cctgggacac ccgcttgtcc tggacgccac aatttctgaa acaacaaaac
     7261 ctgacgatca gtgccgatat tctcaatgta ttggatagca aaaccgctgt tgatacaacg
     7321 aataccggtg tggcgaccta cgccagtggc cgtactttct ggcttgatgt cagcatgaaa
     7381 ttttaatagt tcggatttta cttaaccggg taaattcgcc cggttttcgc atggagataa
     7441 ttatgagaaa cctctgtttc ttactgacgt tagtggcaac tctgttgctc cccgggcggc
     7501 tgattgccgc cgccttaccg caggatgaaa agttaattac cgggcaactg ggcaatggct
     7561 tgcgatatat gatttatccg catgctcatc caaaggatca ggtaaattta tggctgcaaa
     7621 ttcataccgg ttcattgcag gaagaagaca atgagcgcgg cgtggctcat tttgtagaac
     7681 atatgatgtt taacggcaca aaaacatggc cgggtaataa agtcatcgaa acatttgagt
     7741 caatgggcct gcgttttggt cgcgatgtta atgcctatac cagctatgac gaaacggtgt
     7801 atcaggtgag tttgccgact acgcagaaac aaaatctgca acaagtgatg gcaatcttca
     7861 gtgaatggag taatgccgca acctttgaaa aactcgaagt agacgctgaa cgtggcgtaa
     7921 ttactgagga atggcgtgcc catcaggatg cgaaatggcg cacctctcag gcgcgccgcc
     7981 ctttcctgct ggctaatacc cgtaatttag accgtgaacc tatcggcctg atggatactg
     8041 tcgccacggt cacaccggca caattgcgcc aattttatca acgctggtat caaccaaata
     8101 atatgacctt tatcgtggtc ggcgatatcg acagtaaaga agcgctggcg ctgataaagg
     8161 ataatttaag taagcttccg gctaacaaag cagctgaaaa tcgcgtctgg ccgacaaaag
     8221 ccgaaaacca cctgcgcttt aatatcatta atgataaaga aaaccgggtg aacggcatcg
     8281 cactctatta tcgcctgcca atggtacaag tgaacgatga gcaaagcttt atcgaacaag
     8341 ctgaatggag catgttagtt cagctgttca atcaacgtct gcaggaacgc atacagtcgg
     8401 gcgagttgaa gactatttct ggcggcactg cgcgcagcgt taaaattgca cccgattatc
     8461 agtcgctgtt tttccgtgta aatgcacgag acgataatat gcaggaagct gcgaatgcat
     8521 taatggcaga gttggcaacc attgatcagc atggtttttc tgctgaagaa ctcgatgatg
     8581 tcaaatctac ccgcctcacc tggctgaaaa atgcggttga tcagcaagct gaacgtgatt
     8641 tacgtatgct gaccagtcgc ctggcatcca gctcattaaa taatacgccg ttcttgtcgc
     8701 cggaagagac atatcaactt tcgaaacgtc tgtggcagca aattaccgtg caaagtctgg
     8761 cggaaaaatg gcagcagtta agaaagaacc aggacgcatt ttgggagcaa atggtaaaca
     8821 atgagcttgc cgccaaaaaa gcattgtctc ctgcggctat cctggcgctg gaaaaagagt
     8881 acgccaacaa aaagctggcg gcttacatct tcccaggcag aaatttatcg ttaacagtag
     8941 acgctgaccc acaggcggaa attagcagca aagaaacgct ggctgagaat ctgacatcat
     9001 taacactttc caatggtgcc agggttattc tggcaaaatc cgcgggtgaa gagcaaaagc
     9061 tacaaattac tgccgtatcg aataaaggcg atttaagttt ccctgcgcag caaaaatcac
     9121 ttatcgcgct ggcaaataaa gcagtaagcg gaagcggcgt tggtgagctc tcctcttcca
     9181 gcctgaaacg ctggagtgcg gaaaattcgg taaccatgag cagtaaagtc agtggcatga
     9241 atacgttgct ctccgttagc gcgcggacta ataaccctga acctggtttt cagttgatta
     9301 accagcgaat cacccacagc acgattaacg acaatatttg ggcatcgcta caaaatgctc
     9361 aaattcaggc gttgaaaacg ctcgaccagc gtccagcgga gaaattcgcc cagcagatgt
     9421 atgagacgcg ctatgctgat gaccgcacga aattactgca agaaaatcag attgtacagt
     9481 ttactgccgc agatgcgctg gctgccgatc gccagttgtt ttcatctcca gcggatatca
     9541 cgtttgtcat tgtcggtaat gtctcagaag acaaactcgt ggcgttaatt acgcgttact
     9601 taggatcaat caaacactct gattcgccat tagccgcagg taaaccatta actcgcgcga
     9661 cggacaacgc atcggttact gtaaaagaac aaaatgaacc tgtggcacag gtttcacagt
     9721 ggaagcgtta tgattcccgg acacctgtta atctggcgac gcgtatggcg ctcgatgctt
     9781 ttaacgtcgc actggcaaaa gatctacgtg ttaatattcg tgaacaggca tctggagcat
     9841 acagcgtttc ttctcgcctc tcggttgatc ctcaggccaa agatatcagt catttgctgg
     9901 cttttacttg tcaaccagaa cgacatgatg aactgttaac gttagcgaat gaagtgatgg
     9961 ttaagcgtct ggctaaaggg atcagtgagc aagaactgaa tgaataccag caaaacgttc
    10021 agcgcagcct cgatatccaa cagcgtagcg ttcaacaatt agcgaacact attgtaaata
    10081 gtcttattca atatgacgat cctgcagcat ggactgagca ggagcaattg ttgaaacaaa
    10141 tgacggtaga gaatgttaac actgccgtta aacaatatct ttctcatccg gtaaatactt
    10201 ataccggagt attattgcca aaataataac agccccgtca acacatcgtt ggc
//
LOCUS       DAZFRN010000165        10192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFRN010000165 DAZFRN010000000
VERSION     DAZFRN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.167"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            68..1462
                     /gene="galP"
                     /locus_tag="ACHKUB_004175"
     CDS             68..1462
                     /gene="galP"
                     /locus_tag="ACHKUB_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO6657117.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1539..2036
                     /locus_tag="ACHKUB_004176"
     CDS             1539..2036
                     /locus_tag="ACHKUB_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO6657118.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2131..2838
                     /gene="endA"
                     /locus_tag="ACHKUB_004177"
     CDS             2131..2838
                     /gene="endA"
                     /locus_tag="ACHKUB_004177"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO6657119.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2918..3649
                     /gene="rsmE"
                     /locus_tag="ACHKUB_004178"
     CDS             2918..3649
                     /gene="rsmE"
                     /locus_tag="ACHKUB_004178"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO6657120.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3662..4612
                     /gene="gshB"
                     /locus_tag="ACHKUB_004179"
     CDS             3662..4612
                     /gene="gshB"
                     /locus_tag="ACHKUB_004179"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO6657121.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4721..5284
                     /locus_tag="ACHKUB_004180"
     CDS             4721..5284
                     /locus_tag="ACHKUB_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO6657122.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5284..5700
                     /gene="ruvX"
                     /locus_tag="ACHKUB_004181"
     CDS             5284..5700
                     /gene="ruvX"
                     /locus_tag="ACHKUB_004181"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO6657123.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5876..6856)
                     /locus_tag="ACHKUB_004182"
     CDS             complement(5876..6856)
                     /locus_tag="ACHKUB_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO6657124.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6874..7578
                     /gene="yggS"
                     /locus_tag="ACHKUB_004183"
     CDS             6874..7578
                     /gene="yggS"
                     /locus_tag="ACHKUB_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO6657125.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7596..8162
                     /gene="yggT"
                     /locus_tag="ACHKUB_004184"
     CDS             7596..8162
                     /gene="yggT"
                     /locus_tag="ACHKUB_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO6657126.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8159..8449
                     /gene="yggU"
                     /locus_tag="ACHKUB_004185"
     CDS             8159..8449
                     /gene="yggU"
                     /locus_tag="ACHKUB_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO6657127.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8457..9050
                     /locus_tag="ACHKUB_004186"
     CDS             8457..9050
                     /locus_tag="ACHKUB_004186"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO6657128.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDNQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9043..10179
                     /gene="hemW"
                     /locus_tag="ACHKUB_004187"
     CDS             9043..10179
                     /gene="hemW"
                     /locus_tag="ACHKUB_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO6657129.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2395 a         2694 c         2700 g         2403 t
ORIGIN      
        1 cacatctttt tacgtcgtac tcacctatct taattcacaa taaaaaataa ccatattgga
       61 gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga cgtttttcgt
      121 ctgcttcctt gccgctctgg cgggattact ctttggcctg gatatcggtg taattgctgg
      181 cgcactgccg tttattgcag atgaattcca gattacttcg cacacgcaag aatgggtcgt
      241 aagctccatg atgttcggtg cggcagtcgg tgcggtgggc agcggctggc tctcctttaa
      301 actcgggcgc aaaaagagcc tgatgatcgg cgcaattttg tttgttgccg gttcgctgtt
      361 ctctgcggct gcgccaaacg ttgaagtact gattctttcc cgcgttctac tggggctggc
      421 ggtgggtgtg gcctcttata ccgcaccgct gtacctctct gaaattgcgc cggaaaaaat
      481 tcgtggcagt atgatctcga tgtatcagtt gatgatcact atcgggatcc tcggtgctta
      541 tctttctgat accgccttca gctacaccgg tgcatggcgc tggatgctgg gtgtgattat
      601 catcccggca attttgctgc tgattggtgt cttcttcctg ccagacagcc cacgttggtt
      661 tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg ctacgcctgc gtgacaccag
      721 cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt ttgcaggtta aacagagtgg
      781 ctgggcgctg tttaaagaga acagcaactt ccgccgcgcg gtgttccttg gcgtactgtt
      841 gcaggtaatg cagcaattca ccgggatgaa cgtcatcatg tattacgcgc cgaaaatctt
      901 cgaactggcg ggttatacca acactaccga gcaaatgtgg gggaccgtga ttgtcggcct
      961 gaccaacgta cttgccacct ttatcgcaat cggccttgtt gaccgctggg gacgtaaacc
     1021 aacgctaacg ctgggcttcc tggtgatggc tgctggcatg ggcgtactcg gtacaatgat
     1081 gcatatcggt attcactctc cgtcagcgca gtatttcgcc atcgccatgc tgctgatgtt
     1141 tattgtcggt tttgccatga gtgccggtcc gctgatttgg gtactgtgct ccgaaattca
     1201 gccgctgaaa ggccgcgatt ttggcatcac ctgctccacc gccaccaact ggattgccaa
     1261 catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg ctgggtaacg ccaacacctt
     1321 ctgggtgtat gcggctctga acgtactgtt tatcctgctg acattgtggc tagtaccgga
     1381 aaccaaacac gtttcgctgg aacatattga acgtaatctg atgaaaggtc gtaaactgcg
     1441 cgaaatcggc gctcacgatt aatctcctca ggcttcctcc cgtcgcggag gaagccacct
     1501 cttgcagtca tcttttcttc gctctatcct ctgccgctat gaaaacatcc cgtctcccta
     1561 tcgccatcca acaggccgtt atgcgccgcc tgcgggaaaa actcgcccag gccaacctga
     1621 agctagggcg taactacccg gagccaaaac tctcttacac ccagcgcgga acctccgccg
     1681 gaacggcctg gctggaaagc tatgaaattc gcctcaatcc cgttttgctg ttggaaaaca
     1741 gtgaagcttt tattgaagaa gtggtaccgc acgaactggc acatttgctg gtatggaaac
     1801 atttcggccg cgtagcgcca catggcaaag agtggaagtg gatgatggaa agcgtgctgg
     1861 gtgttcccgc ccgtcgtacg catcagttcg aactgcaatc cgtgcgtcgc aacaccttcc
     1921 cctaccgctg caagtgccag gagcatcagc ttaccgtacg ccgccataat cgcgtagttc
     1981 gtggcgaggc cgtctatcgc tgtgttcact gcggtgaaca gctggttgcg aaataaccat
     2041 ctgaactatc aggaactttc ctgatctggc tgattgcata ccaaaacaac tttcgctacg
     2101 ttgctggctc gttttaacac ggagtaagtg atgtaccgtt atttgtctat tgctgcggtg
     2161 gtactgagcg cagcattttc cggcccggcg ttggccgaag gtatcaatag tttttctcag
     2221 gcgaaagccg cggcggtaaa agtccacgct gacgcgcccg gtacgtttta ttgcggatgt
     2281 aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc aatcgtgcgg ctatcaggtg
     2341 cgcaaaaatg aaaaccgagc cagccgcgta gagtgggaac acgtcgttcc cgcctggcag
     2401 ttcggtcacc agcgccagtg ctggcaggac ggtggacgta aaaactgcgc taaagatccg
     2461 gtctatcgca agatggaaag cgatatgcat aacctgcagc cgtcagtcgg tgaggtgaat
     2521 ggcgatcgcg gcaactttat gtacagccag tggaatggcg gcgaaggcca gtacggtcaa
     2581 tgcgccatga aggtcgattt caaagaaaaa gctgccgaac caccagcgcg tgcacgcggt
     2641 gccattgcac gcacctactt ctatatgcgc gaccaataca acctgacact ctctcgccag
     2701 caaacgcagc tgttcaacgc atggaacaag atgtatccgg ttaccgactg ggagtgcgag
     2761 cgcgatgaac gcatcgcgaa ggtgcaaggc aatcataatc cgtatgtgca acgcgcttgc
     2821 caggcgcgaa agagctaacc tacactagcg ggactctttt tgttaacccc taccccacgc
     2881 gtacaaccgc gtggggagac gacgcggatt tttaactatg cgtatccccc gcatttatca
     2941 tcctgaacca ctgaccagcc attctcacat cgcgctttgc gaagatgccg ccaaccatat
     3001 cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa ttgtttgacg gtagcaacca
     3061 ggtctttgac gccgaaatta ccagcgccag caaaaaaagc gtggaagtga aggtgctgga
     3121 aggcaagatc gacgatcgcg aatctccgct gcatattcac ctcggtcagg tgatgtcgcg
     3181 tggtgaaaaa atggaattta cgatccagaa atcgatcgaa ctcggtgtaa gcctcattac
     3241 gccacttttt tctgagcgct gcggcgttaa actggatagt gaacgtctga acaagaagct
     3301 ccagcagtgg cagaagattg caattgctgc ctgtgagcag tgtggtcgta accgggtgcc
     3361 ggaaatccgc ccagcgatgg atctggaagc ctggtgtgca gagcaggatg aaggactgaa
     3421 actgaatctt cacccgcgcg ccagtaacag catcaatacg ttgccgttac cggttgaacg
     3481 cgtccgcctg ctgattggcc cggaaggcgg tttatcggca gatgaaattg ccatgactgc
     3541 ccgctatcaa tttactgata tcctgttggg acctcgcgtt ttgcgtacag agacaactgc
     3601 gctcaccgcc attaccgcgc tacaagtacg atttggcgat ttgggctaac ggagaagaat
     3661 aatgatcaag ctcggcatcg tgatggaccc catcgcaaac atcaacatca agaaagattc
     3721 cagtttcgct atgttgctgg aagcacagcg tcgtggttac gaacttcact atatggagat
     3781 gggcgatctg tatctgatca atggcgaagc ccgcgcccat acccgcacgc tgaacgtgaa
     3841 gcagaactac gaagagtggt tttcattcgt cggtgaacag gatctgccgc tggccgatct
     3901 cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc gagtttatct acgcgaccta
     3961 tattctggaa cgtgccgaag agaaagggac gctgatcgtt aacaagccgc agagcctgcg
     4021 cgactgtaac gagaaactgt ttaccgcctg gttctctgac ttaacgccag aaacgctggt
     4081 tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa cacagcgaca tcattcttaa
     4141 gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg aaagaaggcg atccaaacct
     4201 cggcgtgatt gccgaaaccc tgactgagca tggcactcgc tactgcatgg cgcaaaatta
     4261 cctgccagcc attaaagatg gcgacaaacg cgtgctggtg gtggatggcg agccggtacc
     4321 gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt ggcaatctgg ctgccggtgg
     4381 tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa atcgcccgtc agatcgggcc
     4441 gacgctgaaa gaaaaagggc tgatttttgt tggtctggat atcatcggcg accgtctgac
     4501 tgaaattaac gtcaccagcc caacctgtat tcgtgagatt gaagcagagt ttccggtgtc
     4561 gatcaccgga atgttaatgg atgccatcga agcacgttta cagcagcagt aacccacctt
     4621 agcgagaagg atctcgttga gactctgagt gacagcgccc ttctttccac gcatactggg
     4681 cgctgttgct tttttgaacc aggaaacaga acctctgaca atgaatttac agcatcactt
     4741 tcttattgcc atgcctgctc tccaggatcc gattttccgt cgttccgtgg tctacatttg
     4801 cgaacataat accaatggtg caatggggat catcgtcaac aagccgctgg aaaatctcaa
     4861 aattgaaggg attctggaaa agctgaagat cacgccggag ccgcgtgatg aatcaatccg
     4921 tctggataaa ccggttatgc tcggcggtcc gctggctgaa gatcgcgggt ttattttgca
     4981 tactccgccc tccaattttg cttccagcat tcgcatttca gacaacacag tgatgaccac
     5041 ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa ccgtctgacg tattggtggc
     5101 tctgggttat gcctcctggg aaaaaggtca actggaacaa gaaattctcg ataacgcgtg
     5161 gctaacggcc ccggcggatc tgaatattct gttcaaaacg ccgattgccg accgctggcg
     5221 cgaggcggca aaactgattg gtgtggatat tctcaccatg cctggtgtgg cagggcacgc
     5281 ctgatgagtg gaaccttact cgccttcgac ttcggcacca aaagcattgg cgtagcggtc
     5341 ggccaacgca ttaccggcac tgctcgccct ttgcctgcaa ttaaagcaca ggacggtacg
     5401 ccggactgga acctcatcga acgtttactg aaagagtggc agccggacga aatcatcgtc
     5461 ggtttgccgc tgaatatgga cggcaccgag caaccattga ctgccagagc gcgtaaattt
     5521 gccaaccgta ttcatggtcg tttcggtgtt gaagtaaagc tccatgacga acgtcttagc
     5581 accgtggaag cccgttccgg tctgtttgaa cagggcggct atcgggcgct caacaaaggc
     5641 aaagtcgact ctgcctctgc ggttattatt ctcgaaagct atttcgagca gggatattaa
     5701 ggcgatctaa aagcctggcg gatagtcgtt accgccatct tgccggatgc ggcgtgaacg
     5761 ccttatccag tacacatctg gcagcggctg taggtctgat aagacgcgtt agcgtcgcat
     5821 cagacattta ttgcctttat aggccctata aacttgcgca tcgggcatgg caacgtcaca
     5881 aacgcccttc ccccacccgc tgctgataac tctgctgaaa cgttaacatc cccacctgct
     5941 gcccggtttg aataacatgc ggtaactggt gggttttccc ttcgcgaatc aaattcccca
     6001 ccgcgggagt gttaatcaat aattcaaaca gcgccacgcg tccttcctgt ttatccactt
     6061 ccagcttttg tgacaacacg gcccgtaaac tacctgccag ttgattacgt acgggatctt
     6121 tttcctgcgc cggaaatgaa tccaccagtc gctcaactgc ctgcgcggca ccacgcgtat
     6181 gtaatgttgc cagcaccaaa tgcccggttt ctgccgccgt cagtgccaga cggattgtct
     6241 cgctgtcacg cagctctccg agcaaaatca catcaggatc ttcccgcaat gcggcccgca
     6301 atcccgatgc gaacgtcata cagtgcaaac caatttcccg ctgctggatc aaacatcgct
     6361 ggctggcata gagatattcc acaggatctt ccagcgtcag aatatgcgca tcggcatgtt
     6421 gattaagata gccaaccatc gccgccagcg tggtagattt gccactcccc gtcgcccccg
     6481 tcaccagaat caggccattc tcgctcttga gtaattccgg caataccggt ggcgcaccaa
     6541 gctgttcgag ctgtggacag tgcgaaggta acaaccgtaa tgccagagaa ataccttgcc
     6601 gttgcgcgaa cgcactgcca cgcaaccgct ggttttccgc cagcgacacg gcaaaatcca
     6661 gctggccatt ttccagcaat attgcccgct gatcgtcatc cagccactcc cgcagtagct
     6721 cttcgacgtc cggcgcatca aacggcgcag cttccattag cccgcgaatg cgccatcgtg
     6781 cgggccaggc gctgcacagg tgtagatccg agacattatg ctttacacta agggccacaa
     6841 tttcttccat attcatacta agatcctcgg aaaatgaacg atattgcgca taacctggca
     6901 caggtccggg acaaaatctc agctgctgca acgcgttgcg gccgttctcc agaagaaatt
     6961 acgctgcttg cagtcagtaa aacaaaacct gcgagcgcca tcgcagaagc cattgatgcc
     7021 gggcagcgtc aatttggtga aaactacgtt caggaagggg tagataaaat tcgccacttt
     7081 caggaactgg gcgtaacagg attagaatgg cattttattg gcccgttgca gtctaataaa
     7141 agccgcctgg tggcagagca tttcgactgg tgtcatacca tcgaccgttt gcgcattgct
     7201 acccgcctca acgatcagcg cccggcagaa cttccccctc ttaacgtact gattcaaatt
     7261 aatattagtg atgaaaacag taagtccggg attcaactgg cagaactgga cgagctggca
     7321 gcggcggtcg ctgaactacc gcgtttacgt ctgcgcgggc tgatggcaat ccctgcgcct
     7381 gagtcagaat atgtaaggca gtttgaagtt gcacgccaaa tggctgtagc atttgccgga
     7441 ctgaaaacgc gctacccgca tatcgacacg ctctctctgg gaatgtcgga cgatatggaa
     7501 gccgccattg cggcaggtag cacgatggtt cgtatcggca ctgcaatttt tggtgcgcgt
     7561 gattactcta aaaaataagg aattaaagga acgccatgaa tacgttgact ttcctgcttt
     7621 caacggtcat tgagctgtat accatggtgc tgttattacg catctggatg cagtgggctc
     7681 attgtgattt ttacaacccc ttctcacagt ttgtagtgaa ggtaacgcag ccaattatcg
     7741 ggccactgcg ccgcgttatt ccggcaatgg gtcccattga tagcgcctcg ctgctggttg
     7801 cgtacattct cagttttatc aaagccatcg tgctgtttaa agtggtgacc ttcctgccaa
     7861 tcatctggat tgccggttta ctgattctgc tgaaaaccat cggcctgctg attttctggg
     7921 tcctgctggt gatggcgatt atgagctggg taagccaggg tcgtagtccg attgaatacg
     7981 tgctgattca gctggccgat ccgctgctgc gcccgattcg ccgcctgcta ccggcaatgg
     8041 gtgggattga tttctcgccg atgatcctcg ttctgctgct gtatgtcatc aatatgggtg
     8101 tcgcagaagt attacaggca accggaaaca tgctgctgcc ggggctgtgg atggcgttat
     8161 gagtgccgta acagttaatg atgacggtct ggttttacgg ctttatattc agccgaaagc
     8221 cagccgtgat tctattgtcg gtttacatgg cgacgaagtt aaagtcgcca ttaccgcgcc
     8281 gccggttgac ggtcaggcca acagccatct ggtgaagttt ctcggtaagc aattccgggt
     8341 tgccaaaagc caggtggtga ttgaaaaagg cgaacttggc cgccacaaac aaattaaaat
     8401 cattaatccg caacaaatcc cgccagaaat cgcggcgtta attaattagg tatcctatgc
     8461 aaaaagttgt cctcgcaacc ggcaatgccg gtaaagtgcg tgagctggcg tcgctgctta
     8521 gcgacttcgg tcttgatatc gtggcccaaa cagacctcgg cgtagattcc gctgaagaaa
     8581 ccggcctgac ctttatcgaa aacgcgattc tgaaagcgcg ccatgcggca aaagtgaccg
     8641 gtttaccggc aattgccgac gactctggtc tggcggtaga tgtacttggc ggcgcgccgg
     8701 gcatttactc cgcgcgttat tccggtgaag acgcgaccga tcaaaagaat ctgcaaaaac
     8761 tgctggaaac actaaaagac gtaccggaca accaacgtca ggcgcgtttc cactgcgtac
     8821 tggtatatct gcgtcacgcg gaagatccca ctccgctggt gtgccacggc agctggccgg
     8881 gcatgattac tcgtgaacca gcgggtactg gcggctttgg ttatgatcca atcttcttcg
     8941 taccttccga agggaaaacc gctgccgaac tgacccgcga agaaaagagc gccatttccc
     9001 accgtggtca ggcattgaaa ctgctgctgg acgctttacg taatggttaa attaccgccg
     9061 ctaagtctct acattcacat cccgtggtgc gtgcagaaat gcccgtactg cgatttcaac
     9121 tctcacgcgt tgaaaggaga agtgccgcac gacgattatg ttcagcatct gcttaacgac
     9181 ctggacaacg atgtggctta cgctcagggc cgtgaagtaa agacaatctt tattggcggt
     9241 ggtacgccga gcctgctttc cggcccggcg atgcaaacgc tgctggacgg cgtgcgtgcg
     9301 cgtttgccgc tggcagcgga tgcagaaatt actatggaag cgaacccagg cacggtagaa
     9361 gcggatcgct ttgtcgatta tcagcgtgct ggtgtgaacc gcatctctat tggtgtgcag
     9421 agttttagcg aagaaaagct gaaacgactt gggcgtattc atggcccgca agaagcgaaa
     9481 cgagcggcaa agctggcgag cggtttaggg ttacgtagct ttaaccttga tttgatgcat
     9541 ggactaccgg atcaatcact ggaagaggcg cttggcgatc tacgccaggc cattgaactg
     9601 aatccgccgc atctttcctg gtatcaactg accatcgaac ccaatacgct gtttggttcg
     9661 cgaccaccgg tgctgccgga cgacgatgcg ttgtgggata ttttcgaaca ggggcatcag
     9721 ttattaaccg cagcgggtta tcagcaatat gaaacttccg cttacgccaa acccggttat
     9781 cagtgccagc acaatctcaa ctactggcgc tttggcgact acatcggtat tggctgcggc
     9841 gcgcacggga aagtgacctt cccggatggg cgcattctgc gtaccaccaa aacgcgtcat
     9901 ccgcgtggtt ttatgcaagg aaggtatctg gaaagccagc gtgatgtcga agccgcagat
     9961 aagccgtttg agttctttat gaatcgcttc cgtctgctgg aggccgcgcc gcgcgtggag
    10021 tttattgcgt ataccgggct ttgcgaagat gtgattcgcc cacagttaga cgaggcgatt
    10081 gcccagggtt atctcacaga atgtgcggat tactggcaga taacggaaca tggtaaatta
    10141 ttcttaaact cacttcttga attattcctc gctgagtaat cattaatgcg tc
//
LOCUS       DAZFRN010000166        10143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFRN010000166 DAZFRN010000000
VERSION     DAZFRN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.168"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(81..857)
                     /gene="yaaA"
                     /locus_tag="ACHKUB_004188"
     CDS             complement(81..857)
                     /gene="yaaA"
                     /locus_tag="ACHKUB_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO6657130.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(927..2357)
                     /locus_tag="ACHKUB_004189"
     CDS             complement(927..2357)
                     /locus_tag="ACHKUB_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO6657131.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2636..3589
                     /gene="tal"
                     /locus_tag="ACHKUB_004190"
     CDS             2636..3589
                     /gene="tal"
                     /locus_tag="ACHKUB_004190"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6657132.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3704..4291
                     /gene="mog"
                     /locus_tag="ACHKUB_004191"
     CDS             3704..4291
                     /gene="mog"
                     /locus_tag="ACHKUB_004191"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO6657133.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4326..4892)
                     /gene="satP"
                     /locus_tag="ACHKUB_004192"
     CDS             complement(4326..4892)
                     /gene="satP"
                     /locus_tag="ACHKUB_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO6657134.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5041..5754)
                     /gene="msyB"
                     /locus_tag="ACHKUB_004193"
     CDS             complement(5041..5754)
                     /gene="msyB"
                     /locus_tag="ACHKUB_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6657135.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5780..6184)
                     /locus_tag="ACHKUB_004194"
     CDS             complement(5780..6184)
                     /locus_tag="ACHKUB_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO6657136.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6561..8477
                     /gene="dnaK"
                     /locus_tag="ACHKUB_004195"
     CDS             6561..8477
                     /gene="dnaK"
                     /locus_tag="ACHKUB_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO6657137.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8566..9696
                     /gene="dnaJ"
                     /locus_tag="ACHKUB_004196"
     CDS             8566..9696
                     /gene="dnaJ"
                     /locus_tag="ACHKUB_004196"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO6657138.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9800..9952)
                     /gene="mokC"
                     /locus_tag="ACHKUB_004197"
     CDS             complement(9800..9952)
                     /gene="mokC"
                     /locus_tag="ACHKUB_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO6657139.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2658 a         2546 c         2630 g         2309 t
ORIGIN      
        1 agtacctgta atatattgaa tctgcaggac tttgtaggcc ggataaggcg ttcacgccgc
       61 atccggcatt gactacaaac ttaacgctgc tcgtagcgtt taaacaccag ttcgccattg
      121 ctggaggaag cttcatcaaa gaagtaacct tcgctattaa aaccagtcag ttgctctggt
      181 ttggtcagcc gattttcaat aataaaacga ctcatcagac cgcgtgcttt cttagcgtag
      241 aagctgatta tcttaaattt gccgttcttc tcatcgagga acaccggctt gataatctcg
      301 gcattcaatt tcttcggctt caccgattta aaatactcat ctgacgccag attaatcacc
      361 acattatcgc cttgtgctgc gagcgcctcg ttcagcttgt tggtgatgat atctccccag
      421 aattgataca gatctttccc tcgggcattc tcaagacgga tccccatttc cagacgataa
      481 ggctgcatta aatcgagcgg gcgcagtacg ccatacaagc cggaaagcat tcgcaaatgc
      541 tgttgggcaa aatcgaaatc gtcttcgctg aaggtttcgg cctgcaagcc ggtgtagaca
      601 tcacctttaa acgccagaat cgcctggcgg gcattctccg gcgtgaaatc tggctgccag
      661 tcatgaaagc gagcggcgtt gatacccgcc agtttgtcgc tgatgcgcat cagcgtgcta
      721 atctgcggag gcgtcagttt ccgcgcttca tggatcaact gctgggaatt gtctaacagc
      781 tccggcagcg tatagcgcgt ggtggtcaac gggctttggt aatcaagcgt tttcgcaggt
      841 gaaataagaa tcagcatatc cagtccttgc aggaaattta tgccgacttt agcaaaaaaa
      901 gagaatgagt tgatcgatag ttgtgattac tcctgcgaaa catcatccca cgcgtccgga
      961 gaaagctggc gaccgatatc cggataacgc aacggatcaa acaccgggcg cacgccgagt
     1021 ttacgctggc gtagataatc actggcaatg gtatgaacca caggcgagag cagtaaaatg
     1081 gcggtcaaat tggtaatagc catgcaggcc attatgatat ctgccagttg ccacatcagc
     1141 ggaaggctta gcaaggtgcc gccgatgacc gttgcgaagg tgcagatccg caaacaccag
     1201 atcgctttag ggttgttcag gcgtaaaaag aagagattgt tttcggcata aatgtagttg
     1261 gcaacgatgg agctgaaggc aaacagaata accacgaggg taacaaactc agcaccccag
     1321 gaacccatta gcacccgcat cgccttctgg ataagctgaa taccttccag cggcatgtag
     1381 gttgtgtcgt tacccgccag taatatcagc atggcgcttg ccgtacagat gaccagggtg
     1441 tcgataaaaa tgccaatcat ctggacaatc ccttgcgctg ccggatgcgg aggccaggac
     1501 gccgctgccg ctgccgcgtt tggcgtcgaa cccattcccg cctcattgga aaacatactg
     1561 cgctgaaaac cgttagtaat cgcctggctt aaggtatatc ccgccgcgcc gcctgccgct
     1621 tcctgccagc caaaagcact ctcaaaaata gaccaaatga cgtggggaag ttgcccgata
     1681 tttattacgc aaatcaccat gctggtcagc acccagatta tcgccatcaa cgggacaaac
     1741 ccctgcatga gccgggcgac gccatgaaga ccgcgagtga ttgccagcag agcaaagaca
     1801 gcgagaataa tgcctgtcac cagcggagga aaatcaaaag aaaaacttag ggcgcgggca
     1861 acggcgttcg cctgaactcc gctgaaaatt atgccatagg cgatgagcaa aaagacggcg
     1921 aacagaacgc ccatccaacg catccccagc ccgcgcgcca tataccatgc cggtccgcca
     1981 cgaaactgtc cattgacgtc acgttcttta taaagttgtg ccagagaaca ttcggcaaac
     2041 gaggtcgcca tgccgataaa cgcggcaacc cacatccaaa agacggctcc aggtccaccg
     2101 gcggtaatag ccagcgcaac gccggccagg ttgccgctac ccacgcgcgc cgcaagactg
     2161 gtacacaatg actgaaatga ggttaaaccg cctggctgtg gatgaatgct atttttaaga
     2221 cttttgccaa actggcggat gtagcgaaac tgcacaaatc cggtgcgaaa agtgaaccaa
     2281 caacctgcgc cgaagagcag gtaaatcatt accgatcccc aaaggacgct gttaataaag
     2341 gagaaaaaat ctggcatgca tatccctctt attgccggtc gcgatgactt tcctgtgtaa
     2401 acgttaccaa ttgtttaaga agtatatacg ctacgaggta cttgataact tctgcgtagc
     2461 atacatgagg ttttgtataa aaatggcggg cgatatcaac gcagtgtcag aaatccgaaa
     2521 cagtctcgcc tggcgataac cgtcttgtcg gcggttgcgc tgacgttgcg tcgtgatatc
     2581 atcagggcag accggttaca tccccctaac aagctgttta aagagaaata ctatcatgac
     2641 ggacaaattg acctcccttc gtcagtacac caccgtagtg gccgacactg gggacatcgc
     2701 ggcaatgaag ctgtatcaac cgcaggatgc cacaaccaac ccttctctca ttcttaacgc
     2761 agcgcagatt ccggaatacc gtaagttgat tgatgatgct gtcgcctggg cgaaacagca
     2821 gagcaacgat cgcgcgcagc agatcgtgga cgcgaccgac aaactggcag taaatattgg
     2881 tctggaaatc ctgaaactgg ttccgggccg tatctcaact gaagttgatg cgcgtctttc
     2941 ctatgacact gaagcgtcaa ttgcgaaagc aaaacgcctg atcaaactct acaacgatgc
     3001 tggtattagc aacgatcgta ttctgatcaa actggcttct acctggcagg gtatccgtgc
     3061 tgcagaacag ctggaaaaag aaggcatcaa ctgtaacctg accctgctgt tctccttcgc
     3121 tcaggctcgt gcttgtgcgg aagcaggcgt gttcctgatc tcgccatttg ttggccgtat
     3181 tcttgactgg tacaaagcga atactgataa gaaagagtac gctccggcag aagatccggg
     3241 cgtggtttct gtatctgaaa tctaccagta ctacaaagag cacggttatg aaaccgtggt
     3301 tatgggcgca agcttccgta acatcggcga aattctggaa ctggcaggct gcgaccgtct
     3361 taccatcgca cctacactgc tgaaagagct ggcggagagc gaaggggcta tcgaacgtaa
     3421 actgtcttac accggcgaag tgaaagcgcg tccggcgcgt atcactgagt ccgagttcct
     3481 gtggcagcac aaccaggatc caatggcagt agataaactg gcggaaggta tccgtaagtt
     3541 tgctattgac caggaaaaac tggaaaaaat gatcggcgat ctgctgtaat cattcttagc
     3601 gtgaccggga agtcggtcac gctacctctt ctgaagcctg tctgtcactc ccttcgcagt
     3661 gtatcattct gtttaacgag actgtttaaa cggaaaaatc ttgatgaata ctttacgtat
     3721 tggcttagtt tccatctctg atcgcgcatc cagcggcgtt tatcaggata aaggcatccc
     3781 tgcgctggaa gaatggctga catcggcgct aaccacgccg tttgaactgg aaacccgctt
     3841 aatccccgat gagcaggcga tcatcgagca aacgttgtgt gagctagtgg atgaaatgag
     3901 ttgccatctg gtgctcacca cgggcggaac tggcccggcg cgtcgtgacg taacgcccga
     3961 tgcgacgctg gcagtagcgg accgcgagat gcctggcttt ggtgaacaga tgcgccagat
     4021 aagcctgcat tttgtaccaa ctgcgatcct ttcgcgtcag gttggtgtga ttcgcaaaca
     4081 ggcgctgatc cttaacttac ccggtcagcc gaagtctatt aaagagacgc tggaaggtgt
     4141 gaaggacgct gcgggtaacg ttgtggtaca cggtattttt gccagcgtac cgtactgcat
     4201 tcagttgctg gaagggccat acgttgaaac ggcaccggaa gtggttgcag cattcagacc
     4261 gaagagtgca agacgcgacg ttagcgaata aaaaaatccc cccgagcggg gggatctcaa
     4321 aacaattagt gggattcacc aatcggcaga acggtgcgac caaactgctc gttcagtact
     4381 tcacccatcg ccagatagat tgcgctggca ccgcagatca gcccaatcca gccggcaaag
     4441 tggatgattg cggcgttacc ggcaatgtta ccgatcgcca gcagggcaaa cagcacggtc
     4501 aggctaaaga aaacgaattg cagaacgcgt gcgcctttca gcgtgccgaa gaacataaac
     4561 agcgtaaata cgccccacag acccaggtag acaccaagga actgtgcatt tggcgcatcg
     4621 gtcagaccca gtttcggcat cagcagaatc gcaaccagcg tcagccagaa agaaccgtaa
     4681 gaggtgaatg cggttaaacc gaaagtgttg ccttttttgt actccagcag accagcaaaa
     4741 atttgcgcga tgccgccgta gaaaatgccc atggcaagaa taataccgtc cagagcgaaa
     4801 taacccacgt tgtgcaggtt aagcagaatg gtggtcatgc cgaagcccat caggcccagc
     4861 ggtgccggat tagccaactt agtgttgccc ataattcctc aaaaatcatc atcgaatgaa
     4921 tggtgaaata atttccctga ataactgtag tgttttcagg gcgcggcata ataatcagcc
     4981 agtggggcag tgtctacgat cttttgaggg gaaaatgaaa attttccccg gtttccggta
     5041 tcagacctga gtggcgctaa ccatccggcg caggcaggcg atttgcagta cggctggaat
     5101 cgtcacgcga taggcgctgc cgctgaccgc tttaacccca tttagtgccg cacctacagg
     5161 gcctcccagc cccgcgccgc gcagcaaacc atgcccaagt acgctcattg ctgcgtgggt
     5221 gcgtaaaatg cgggtcagtt ggctggaaag caaatgcgac acgccttttg ccaataattt
     5281 gtctttcatc agcagcggca gcagctcttc cagctcattc accctggcat cgatcgcgtg
     5341 cagaaactcc tgcttatgtt cctcgtccat attcttccag gtgttacgca gaaattgttc
     5401 cagtaactgt tgctcaatct caaacgtaga catctctttg tcggctttca gcttcaatcg
     5461 ttttgaaaca tcgagcaaaa tggcccgata caatttaccg tgtccgcgca gtttgttggc
     5521 gatactatcg ccaccaaaat gctgtaattc tccagcaatc agctgccagt tgcggcgatg
     5581 ttgctcggga tgcccttcca tcgatttaaa cagttcgttg cgcatcagta cgctggagag
     5641 gcgagttttg cctttttcat tatgggtgag caatcgggcg aaatttgcca actgttcctc
     5701 actgcaatgc tggagaaaat ccagatctga atcattcagg taattaacat tcattttttg
     5761 tggcttctat attctggcgt tagtcgtcgc cgataatttt cagcgtggcc atatccgatg
     5821 agttcaccgt atgaccggaa aaggtgattt ttgagacgca gcgtttattg tcgttatcgc
     5881 tgttaatgtt gatccagtca gtggtttgcc cttcttttat ttctgaagga atattcaggc
     5941 tctgactggc gctacgggcg gctttgaaat aaaccgatgc accgcttaac tgtaaatcgc
     6001 catggtcggc agagagttgt atgcgtttaa caattcggca aacaggaagt ttcagcgcca
     6061 gatcgttggt ttcgttacgt ggcatcgcaa tgacgccgag gattttatgg tcgtttgcct
     6121 gcgccgtgca gcacagcatc agactaatcg ccaggctggc ggaaatcgta aaaacggatt
     6181 tcataaggat tctcttagtg gggagaggta gggggatgga tacccactag tttactgctg
     6241 ataaagagaa gattcaggtg cgtaatgttt tctttttatt acaatttttt gatgaatgtc
     6301 ttggctgcga ttcattcttt atatgaataa aattgctgtc agtttcacgt cttgtcctgc
     6361 tatatcgcga aatttctgcg caaaagcaca aaaaattttt gcatctcccc cttgatgacg
     6421 tgggttacga ccccatttag tagtcaaccg cagtgagtga gtctgcaaaa aaatgaaatt
     6481 gggcagttga aaccagacgt ttcgccccta ttacagactc acaaccacat gatgaccgaa
     6541 tatatagtgg agacgtttag atgggtaaaa taattggtat cgacctgggt actaccaact
     6601 cttgtgtagc gattatggat ggcaccactc ctcgcgtgct ggagaacgcc gaaggcgatc
     6661 gcaccacgcc ttctattatt gcctataccc aggatggtga aactctggtt ggtcagccgg
     6721 ctaaacgtca ggcagtgacg aacccgcaaa acaccctgtt tgcgattaaa cgcctgattg
     6781 gccgccgctt ccaggacgaa gaagtacagc gtgatgtttc catcatgccg ttcaaaatta
     6841 ttgctgctga taacggcgac gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc
     6901 agatttctgc tgaagtgctg aaaaaaatga agaaaaccgc tgaagattac ttgggtgaac
     6961 cggtaactga agctgttatc accgtaccgg catactttaa cgatgctcag cgtcaggcaa
     7021 ccaaagacgc aggccgtatc gctggtctgg aagtaaaacg tatcatcaac gaaccgaccg
     7081 cagctgcgct ggcttacggt ctggacaaag gtactggcaa ccgtactatc gcggtttatg
     7141 acctgggtgg tggtactttc gatatttcta ttatcgaaat cgacgaagtt gacggcgaaa
     7201 aaaccttcga agttctggct accaacggtg atacccacct gggtggtgaa gacttcgaca
     7261 gccgtctgat caactacctg gttgaagaat tcaagaaaga tcagggcatt gacctgcgca
     7321 acgatccgct ggcaatgcag cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt
     7381 cttccgctca gcagaccgac gttaacctgc cgtacatcac tgcagatgcg accggtccga
     7441 aacacatgaa catcaaagtg actcgtgcga aactggaaag cctggttgaa gatctggtaa
     7501 accgttccat tgagccgctg aaagttgcac tgcaggacgc tggcctgtcc gtatctgata
     7561 tcgacgacgt tatcctcgtt ggtggtcaga ctcgtatgcc aatggttcag aagaaagttg
     7621 ctgagttctt tggtaaagag ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg
     7681 gtgctgctgt tcagggtggt gttctgactg gtgacgtaaa agacgtactg ctgctggacg
     7741 ttaccccgct gtcactgggt atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga
     7801 aaaacaccac tatcccgacc aagcacagcc aggtgttctc taccgctgaa gacaaccagt
     7861 ctgcggtaac catccatgtg ctgcagggtg aacgtaaacg tgcggctgat aacaaatctc
     7921 tgggtcagtt caacctggat ggtatcaacc cggcaccgcg cggcatgccg cagatcgaag
     7981 ttaccttcga tatcgatgct gacggtatcc tgcacgtttc cgcgaaagat aaaaacagcg
     8041 gtaaagagca gaagatcacc atcaaggctt cttctggtct gaacgaagat gaaatccaga
     8101 aaatggtacg cgacgcagaa gctaacgccg aagctgaccg taagtttgaa gagctggtac
     8161 agactcgcaa ccagggcgac catctgctgc acagcacccg taagcaggtt gaagaagcag
     8221 gcgacaaact gccggctgac gacaaaactg ctatcgagtc tgcactgact gcactggaaa
     8281 ctgctctgaa aggtgaagac aaagccgcta tcgaagcgaa aatgcaggag ctggcacagg
     8341 tttcccagaa actgatggaa atcgcccagc agcaacatgc ccagcagcag actgccggtg
     8401 ctgatgcttc tgcaaacaac gcgaaagatg acgatgttgt cgacgctgaa tttgaagaag
     8461 tcaaagacaa aaaataatcg ccctataaac gggtaattat actgacacgg gcgaagggga
     8521 atttcctctc cgcccgtgca ttcatctagg ggcaatttaa aaaagatggc taagcaagat
     8581 tattacgaga ttttaggcgt ttccaaaaca gcggaagagc gtgaaatcaa aaaggcctac
     8641 aaacgcctgg ccatgaaata ccacccggac cgtaaccagg gtgacaaaga ggccgaggcg
     8701 aaatttaaag agatcaagga agcttatgaa gttctgaccg actcgcaaaa acgtgcggca
     8761 tacgatcagt atggtcatgc tgcgtttgag caaggtggta tgggcggcgg cggttttggc
     8821 ggcggcgcag acttcagcga tatttttggt gacgttttcg gcgatatttt tggcggcgga
     8881 cgtggtcgtc aacgtgcggc gcgcggtgct gatttacgct ataacatgga gctcaccctc
     8941 gaagaagctg tacgtggcgt gaccaaagag atccgcattc cgactctgga agagtgtgac
     9001 gtttgccacg gtagcggtgc aaaaccaggt acacagccgc agacctgtcc gacctgtcat
     9061 ggttctggtc aggtgcagat gcgtcaggga ttcttcgctg tacagcagac ctgtccacac
     9121 tgtcagggcc gcggtacgct gatcaaagat ccgtgcaaca aatgtcatgg tcatggtcgt
     9181 gttgagcgca gcaaaacgct gtccgttaaa atcccggcag gggtggacac tggagaccgc
     9241 atccgtcttg cgggcgaagg tgaagcgggc gagcatggcg caccggcagg cgatctgtac
     9301 gttcaggttc aggttaaaca gcacccgatt ttcgagcgtg aaggcaacaa cctctattgc
     9361 gaagtcccga tcaacttcgc tatggcggcg ctgggtggcg aaatcgaagt accgaccctt
     9421 gatggtcgcg tcaaactgaa agtgcctggc gaaacccaga ccggtaagct tttccgtatg
     9481 cgcggtaaag gcgtcaagtc tgtccgcggt ggcgcacagg gtgatttgct gtgccgcgtt
     9541 gtcgtcgaaa caccggtagg cctgaacgag aagcagaaac agctgctgca agagctgcaa
     9601 gaaagcttcg gtggcccaac cggtgagcac aacagcccgc gctcaaagag cttctttgat
     9661 ggtgtgaaga agttttttga cgacctgacc cgctaacctc cccaaaagcc tgcccgtggg
     9721 caggcctggg taaaaatagg gtgcgttgaa gatatgcgag cacctgtaaa gtggcgggga
     9781 tcactccccg ccgttgctct tactcggatt cgtaagccgt gaaaacagca acctccgtct
     9841 ggccagttcg gatgtgaacc tcacagaggt cttttctcgt taccagcgcc gccactacgg
     9901 cggtgataca gatgacgatc agggcgacaa tcatcgcctt atgctgcttc attgctctct
     9961 tctccttgac cttacggtca gtaagaggca ctctacatgt gttcagcata tagggggcct
    10021 cgggttgatg gtaaaatatc actcggggct tttctctatc tgccgttcag ctaatgcctg
    10081 agacagacag cctcaagcac ccgccgctat tatatcgctc tctttaaccc attctgtttt
    10141 atc
//
LOCUS       DAZFRN010000167        10050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFRN010000167 DAZFRN010000000
VERSION     DAZFRN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.169"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            61..176
                     /gene="rrf"
                     /locus_tag="ACHKUB_004198"
     rRNA            61..176
                     /gene="rrf"
                     /locus_tag="ACHKUB_004198"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            191..266
                     /locus_tag="ACHKUB_004199"
     tRNA            191..266
                     /locus_tag="ACHKUB_004199"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:224..226,aa:Thr,seq:ggt)
     gene            306..421
                     /gene="rrf"
                     /locus_tag="ACHKUB_004200"
     rRNA            306..421
                     /gene="rrf"
                     /locus_tag="ACHKUB_004200"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(484..1242)
                     /gene="yhdZ"
                     /locus_tag="ACHKUB_004201"
     CDS             complement(484..1242)
                     /gene="yhdZ"
                     /locus_tag="ACHKUB_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO6657140.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIAVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1250..2353)
                     /gene="yhdY"
                     /locus_tag="ACHKUB_004202"
     CDS             complement(1250..2353)
                     /gene="yhdY"
                     /locus_tag="ACHKUB_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6657141.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2363..3544)
                     /gene="yhdX"
                     /locus_tag="ACHKUB_004203"
     CDS             complement(2363..3544)
                     /gene="yhdX"
                     /locus_tag="ACHKUB_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6657142.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3612..4637)
                     /locus_tag="ACHKUB_004204"
     CDS             complement(3612..4637)
                     /locus_tag="ACHKUB_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6657143.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5068..5289)
                     /locus_tag="ACHKUB_004205"
     CDS             complement(5068..5289)
                     /locus_tag="ACHKUB_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657144.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5542..8646)
                     /gene="acrF"
                     /locus_tag="ACHKUB_004206"
     CDS             complement(5542..8646)
                     /gene="acrF"
                     /locus_tag="ACHKUB_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO6657145.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8658..9815)
                     /gene="acrE"
                     /locus_tag="ACHKUB_004207"
     CDS             complement(8658..9815)
                     /gene="acrE"
                     /locus_tag="ACHKUB_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO6657146.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2673 a         2598 c         2419 g         2360 t
ORIGIN      
        1 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
       61 cctggcggcc ttagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      121 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
      181 aatttagcgt gctgatatgg ctcagttggt agagcgcacc cttggtaagg gtgaggtccc
      241 cagttcgact ctgggtatca gcaccacttt ttaggttaaa gttcggcaga ttagaaaaga
      301 atttgtctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
      361 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccaga
      421 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
      481 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      541 aaattcatca ggtgcagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      601 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      661 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      721 tggctcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      781 ttgctgctga ccaccagaaa tttgtccggg aaacttatgc gcatgttcgg caattctcac
      841 ccgctcaagg taatgcatcg ccagagcttc agcctctttc tttggcatct tgcgtaccca
      901 aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg
      961 aaagaccatt cccacttcct gccggacgcg ctcaatattg cggatatctt cattaagttc
     1021 gatgccatct accgcgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
     1081 ggttgtcgat ttaccggaac ctgaagggcc acacagaacg atccgttctc ccggttgcac
     1141 ggttaaattt atatttttca aaacatggaa ttgtccatac catttattga cgttttccag
     1201 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
     1261 tacgcccggt gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga
     1321 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggtgg
     1381 gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca
     1441 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1501 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1561 ccgcttcata ctgcccttta ggcagtgcct gtaatccgcc tcgcacgact tccgcaacat
     1621 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1681 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1741 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1801 ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1861 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctcc agaccaaaaa
     1921 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1981 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgacaca agcccaatca
     2041 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     2101 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg
     2161 agccaaccca gttagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     2221 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     2281 gcacccacac catcgcgcgg cttgagttat ggctcgctgg gcgcgagggc tgagacagca
     2341 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     2401 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2461 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2521 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2581 acggcggaat aatcactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2641 tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2701 cgcggataat ctcggcgata aatgcagatg tataaaccga aagtgccagc gttaaggctg
     2761 ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat
     2821 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2881 ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2941 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     3001 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     3061 caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     3121 agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt gtggaaagct
     3181 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag
     3241 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
     3301 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     3361 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt actgagattc gttacagtgt
     3421 tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
     3481 aggcacgaac cgtagggttg gcaaaggaga gtgagccttt aacggttgag cggcgatgag
     3541 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgctcgttac
     3601 ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta
     3661 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cattacgctc gaaaatttcc
     3721 gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccaa cttcagatcc
     3781 ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc
     3841 gctttttcat cgacgttctg ggaattgatg cccatctctt cagcattcag catggcgaaa
     3901 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc
     3961 ggttctttag agataacctc cggtaagaca atccattcag ctgggttgct taatttgatg
     4021 cgcagggcat acagttgcga ctgatccgag gccagcgtat cgcagcgacc agattccagt
     4081 gccttcgctg attcgtcaga gcgatcgaaa gtcaccggtg tgtacttcat attgtttgcc
     4141 ttgaaatagt cggcaacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     4201 ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg
     4261 tcgtaataag tgacgccagt aaatgccatt cccatcccgg cgtcgcgaga tgaagtccag
     4321 gtcgtattac gggagagcaa atccacttcc cctgactgta aagccgtgaa gcgttctttt
     4381 gctgtgagtg gggtatattt cactttcgtg tcgtcaccaa atacagcagc ggcaacacca
     4441 cgacaaacat caacatcaat acctgaaaac ttaccgtctg catcggcata agagaaccca
     4501 ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgaacggc atcaagcgtc
     4561 gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tagcggcagc cagtgtggct
     4621 atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg
     4681 gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg ccagcttttg
     4741 cgcccgattt cgcgtcgcaa taagctgagt gtagacagga aataataaaa acagcgcccc
     4801 gcaatggtgc gaaaatgaaa catgcgccac attttggcgc aaaatattta cgcgtgtttt
     4861 actaagcaga ataatgattg ttagcgtgag tatatttctg gtgggaagtg tgaaagttaa
     4921 attgatgcgt tatttcgaaa gcagcgaaat aacgcgatat aacatgctaa atcagcagag
     4981 aattagccag cagaatatag caatatttta tcttatgtaa agatactgta tcgttaaaca
     5041 gagcgcgaaa ctccgcgccc tgttcagtta tttcgacaga gcgatgatcc ccatcgtcag
     5101 gccagcaaca gccgctgccc cgccggcaat agccccagcc gtttcccagt tatcctgagt
     5161 cgtgcctttc atacaggtat tggtgtgaac cagacgaccc tgatcgtcat aaaccggaac
     5221 gcagggcgaa tcgtgagcac agcccgccag caatgcggtg agcaatgcca caggaattaa
     5281 tcttttcatg gtgttacctc agtaataaac gctaacgctt aataagttaa ctctcttatt
     5341 aacgagctga atgccatttt atcaccggag gttataaata ttgcggaatg aataatgaca
     5401 aattatgtga attgtaaaaa tagtgaaggg agcgggaact atagaaagtt tttaaagagt
     5461 gagtggaaga atatcttatt aatttttatg ggtttcactg aaaataataa aggcacccga
     5521 aagtgccttt atgtttctga tttatccttt aaagcaacgg cggatcacca caaagaacac
     5581 cggtacgaag aagattgcca gcaacgttgc agagaccatt ccgcccatta ccccgatacc
     5641 cactgcgttc tgcgcgccac tgccggcacc gttactgata gctagcggta atacgccgag
     5701 aataaaggcg agagaggtca tcaggatagg acgcagacgc atacgtactg ccattagtgt
     5761 cgcttcaaca acacctttac cctctttctc catgagatct ttagcgaact caacaatcaa
     5821 aatagcgttt ttggccgaca agccaattgt cgttagcaag cccaccataa agtagacgtc
     5881 atttttttga ttaaagagtg tcgccgccag cagcacgccg acaatcccta acggcacgac
     5941 caacataacc gagacaggaa ttgaccagct ttcatagagt gcagcaaggc acaggaaaac
     6001 aaccacaaag gaaattgcta ccagagcggg agcctggttt cccgataagc gttcctgata
     6061 cgacataccc gtccagtcat aaccaatgcc agcaggtaat tttgacgcaa ggttttccat
     6121 caacgccatg gcatcgccgg aactggttcc tggcgcggct tccccctgaa tctccattga
     6181 cggcagaccg ttgtagcgtt ccagtcgcgg agagccatac acccaatgtg aagtggtaaa
     6241 ggccgagaat ggcaccattt cgccgttggc gctgcggaca taaagtttat cgacatcttc
     6301 tggcagcata cggaatttgg catccgcctg aacatacacc tttttcacgc gaccacggtc
     6361 gatgaagtcg ttaacgtaag tcccacccaa cgccgttgaa atggtctgat tgatgtcaga
     6421 aagtgaaaca cctaatgcct gcgccttttc ctggtcaact tccagtttaa actgcgcggt
     6481 gtcttccagg ccattaggac gcacgctgac taagctggca ggatgttgcg ccgccatacc
     6541 aagcaactgg ttacgggcct gggttagggc atcgtgaccc agcccagcct gatcaattaa
     6601 ctcaaagtcg aaacccgttg ccgtgcccag ttcaacaatg gctggcatat tgaatggaat
     6661 gacaaaaccg tcgcggatct tgccaaattc cattttggca cgatggatta ccgcttccgc
     6721 actgttttca tcaccattac gctcttccca cggtttcaga ctgacgaagg ccataccggc
     6781 gttttgtgcc tggccgctga agctaaagcc gttaaccgta aagacacttt caacgttcgc
     6841 tttctcgttc ttcagatagt aatccgtcac ttgatccaac actttttgcg tccgctcttg
     6901 cgtcgcgcca gcgggtaact gaatcatggt cagaaagaca ccctgatcct cttcaggtaa
     6961 gaaggaagac ggaagacgta aaaacaacac caccattcct gcaacaatca gcgcatagat
     7021 cagtaaatat cgtcctgtgg aaccgaggat tttgccgacg ctgttggtgt agtggttaac
     7081 gctatgatcg aaggtagtat taaaccagcc gaagaaaccg cccttatttt cgtgatgctc
     7141 agcagagacg ggtttaagca gcgttgcaca taacgcaggg gtaagaatca atgccaccag
     7201 aacagaaagc gccattgccg aaacgatggt gatagagaac tggcgataaa ttgccccagt
     7261 agaaccgccg aagaatgcca tcggaataaa taccgctgac agcaccatcg cgatacccac
     7321 cagtgcgccc tggatttgcg acattgattt ttccgtcgct tcttttggcg ggagcttatc
     7381 ctccatcatc acgcgctcga cgttctccac caccactatc gcatcatcga cgagcagccc
     7441 gatggcaagc accatcccga acatcgttag tgtgttgatg gagtaaccaa aagcggcgag
     7501 gatggcaaac gtccctaaca acaccacggg taccgcaatg gtggggatca gcgttgctcg
     7561 catattctgc aagaacagat acatcaccag gaacaccagc ataatggctt cgaacagcgt
     7621 ttttaccact tcgtgaatag aaagctggac gaatggcgtg gtgtcataag ggtagagaac
     7681 cttcattccc tgcgggaaga atggctgtaa ttccgccagt tttgccttaa tggctttcgc
     7741 ggtatcgaga gcattcgcgc cggttgccag cttaatcccc aggcccgccg ccggttttcc
     7801 gttgatacga gcgataacgt tatagttttc accgccaagt tcaacccgtg cgacatcttt
     7861 caggcgtacc accgagccgt cactgtttac gcgcagggtc actttgccga attcttccgg
     7921 attttttaac cgcgtttgag caataatcga ggcattcaac tgttgccctg gtaacgctgg
     7981 cgttccgccc aactgtccgg cagcgatctg atcgttctgt accttcaact ggttaatcac
     8041 atcaaccggt gtcagtttat atttgtttag cagatcggcg tccagccaga tacgcatcgc
     8101 atactgtgcg ccgaaaagct gtacgtcacc gacgccattc agacggctaa gcgtatcttt
     8161 aacgttagag gccacatagt ccgagatatc gtcctgtgtg gtgtctgggt tatcagagac
     8221 aaagcccgcc accatcaaat agctgctact ggacttttca acactgatcc cctgctgctg
     8281 aacctcctgc ggcagcaacg gcgtggcgag ctggagtttg ttctgcacct gcacttgcgc
     8341 gatatcagga tcggtcccgg actggaaggt aagggtaatt gtcacgctac cggcggaatc
     8401 gctggtggag gacatataca tcaggttatc gataccattc atattctgtt cgataacctg
     8461 cgtcaccgta tcctgcacgg tctgcgcatc agcacctgga tagtttgccg aaacagaaac
     8521 cgcaggcggt gcaatggttg gatactgagc gacgggcaat tgtaggatcg ccagtgcgcc
     8581 cgccatcatc agaataatgg ccagcaccca tgcaaatatc ggtcgtcgaa taaaaaagtt
     8641 tgccatgtca gattacctta cttcgatgca gtatctgcgg gggtatcggt agtggcttta
     8701 acctgctctc ccggacgcgc tttttgcagg ccgctgacaa tgacttgatc gccagatttc
     8761 agaccttcgc taatcaacca tttatcgcca atcgcctgac tggcaacgac agggcgcgct
     8821 tccacctgac ttttatcgtt aacaatcagc acggttgcat caccacgcgg tgtgcggcta
     8881 acgccttgtt gcgggataag aatggcgtca ggttggacgc cttcatcaat ccgtgcgcgc
     8941 acaaacatac ccggcaaaag cgtatgttgc gggttaggga agacggcacg tagggttatg
     9001 gagccagtgc tttcatcaac ggtcacatca gagaattgca gcgtaccttt caggggatag
     9061 gtttgaccgt tttccatgac caactctacg ttgctggtgg cgttttcctt atgcaaattt
     9121 ccttgctcta cggattgctt cagcctcata aaatcgttgc tggattgggt cacatcaacg
     9181 tagataggat cgagctgctg gacagtcgcc agttcagtcg tttgcccatt agtgacaaga
     9241 gcgccttcgg tcacggtcga tttgccgata cgtccgctaa ttggcgcagt cactttggta
     9301 taagcgagat tgatgcgagc gctttcgact gtggcttttg cggcaatcac ggcggcatcg
     9361 gcctggcgag catcagcaat ggcctggtcg tactcctgct ggctgatgta tttcgtaccc
     9421 acgagcggaa cgtaacgttt taccgtcaaa tgcgcgatgg cggcggcggc ttcacttttc
     9481 gccagttcgc ctttcgcgct gtcataattt gcctgatagg tcgcgggatc gatctggtac
     9541 agggactggc ctgcttgcac atcgctgcct tcagtgaaat tgcgattcag tacgatcccg
     9601 ctaacctgtg ggcgaacttc ggctatacgg taagcattgg tgcggcctgg taattcagtc
     9661 ttaacttcta acggggccgt ttttacaata tgaacggtaa cctgcggttc accgacgtga
     9721 gctttctctt ctcccttatc gttacaaccg gcgattaaag ccgcggagat cagaataaag
     9781 gagggcagga ggaaaaacct ggcatgtttc gtcattacta ttcctcaaaa aaccaaaagc
     9841 gcgttattta cccaaaaagg caacgcgtta actcgcagaa agaaaaatac agttcgctat
     9901 cctacaaatt atcattcgtc gatgtaagga atagttatga atacaggcgt ctcaaggcac
     9961 ataaacacaa aaaaagatta atattctact gttttatttt gacgcggatt gaaagaggca
    10021 gaattaaaac atcgtaaatt gaaatatata
//
LOCUS       DAZFRN010000168         9979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFRN010000168 DAZFRN010000000
VERSION     DAZFRN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.170"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(24..641)
                     /gene="yncG"
                     /locus_tag="ACHKUB_004208"
     CDS             complement(24..641)
                     /gene="yncG"
                     /locus_tag="ACHKUB_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO6657147.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPRHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            908..2407
                     /gene="ansP"
                     /locus_tag="ACHKUB_004209"
     CDS             908..2407
                     /gene="ansP"
                     /locus_tag="ACHKUB_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO6657148.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVIFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2522..3583)
                     /gene="yncE"
                     /locus_tag="ACHKUB_004210"
     CDS             complement(2522..3583)
                     /gene="yncE"
                     /locus_tag="ACHKUB_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO6657149.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3825..5927
                     /gene="pqqU"
                     /locus_tag="ACHKUB_004211"
     CDS             3825..5927
                     /gene="pqqU"
                     /locus_tag="ACHKUB_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO6657150.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            complement(5963..6628)
                     /gene="mcbR"
                     /locus_tag="ACHKUB_004212"
     CDS             complement(5963..6628)
                     /gene="mcbR"
                     /locus_tag="ACHKUB_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO6657151.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6826..7863)
                     /gene="curA"
                     /locus_tag="ACHKUB_004213"
     CDS             complement(6826..7863)
                     /gene="curA"
                     /locus_tag="ACHKUB_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO6657152.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8041..8559
                     /gene="mddA"
                     /locus_tag="ACHKUB_004214"
     CDS             8041..8559
                     /gene="mddA"
                     /locus_tag="ACHKUB_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO6657153.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDSVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVITAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8556..9005
                     /gene="ydcZ"
                     /locus_tag="ACHKUB_004215"
     CDS             8556..9005
                     /gene="ydcZ"
                     /locus_tag="ACHKUB_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6657154.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9006..9239)
                     /gene="ydcY"
                     /locus_tag="ACHKUB_004216"
     CDS             complement(9006..9239)
                     /gene="ydcY"
                     /locus_tag="ACHKUB_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO6657155.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9325..9498)
                     /gene="ortT"
                     /locus_tag="ACHKUB_004217"
     CDS             complement(9325..9498)
                     /gene="ortT"
                     /locus_tag="ACHKUB_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO6657156.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9693..9788
                     /gene="yncL"
                     /locus_tag="ACHKUB_004218"
     CDS             9693..9788
                     /gene="yncL"
                     /locus_tag="ACHKUB_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO6657157.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2402 a         2447 c         2440 g         2690 t
ORIGIN      
        1 aacataatcc tctggtatcc cactcaaata atttcattcc tttttaatac ttcttgtaat
       61 ttcggtaact gacatacagc atcagcaatc gcactgatat tcgtggcatt atcctgaaac
      121 cattcatgcc taggccccca tgtgcgcata gtgcaaagat aacagtccac cagcgttagt
      181 tgctcaccaa acgcataagg ttcagcggtc aattgtgaat tcagccagat ataaagcgat
      241 ttacgatatt caattacatt cttctttaac tgttcaggtg cgtcaggagc ccaacgctca
      301 gggtaatcgg cgaaagtgaa tgttggatag acattagcaa ccagccagac caatagccgt
      361 tgaaactgct gacgttcggc gcgcccaacc ggtggcgcaa ggtccgggcg acgatcgaga
      421 accatcaatg caatcgccgc tgtctccgtc ataatctcgt cattttccag cgccagtgtc
      481 ggaatctgac acaacgggtt cagggttttc aataactcgc gtgaagttcc ctcgtggtca
      541 aaaccactga catcaacgaa ctgataagga atatcagcca gtgttagcat cagctcactg
      601 attgtagagc cccatccggg tacgccatac accttaatca tatgcccctc acaatctttt
      661 aaagattaag tgtaggacag tacacaggtt aaccagcaaa aggatttgtc attttttcgt
      721 cagttgtatt aatccccttc aggattgtaa ggggatgttt tgcatcgttt atcgataacg
      781 ggtaggatgc tgcgccgata gttttatacc cataaagaat aatggtgata actatcatcg
      841 ccaggatgaa taaacattgt tcatggcaac ttatatgact ttttcattaa agcaatcagg
      901 gagagcaatg agtaaacacg acaccgacac ttcagatcaa cacgccgcga aacgccgctg
      961 gcttaatgcc cacgaagagg ggtatcacaa agcgatgggc aatcgccagg tacagatgat
     1021 cgccattggc ggcgcgattg gcaccggctt gtttttaggt gcaggagccc gactgcaaat
     1081 ggcgggtcca gcactggcac tggtttattt aatttgtggc ttgttttcgt tttttattct
     1141 gcgtgcattg ggtgagctgg tgctacaccg cccttccagt ggcagttttg tctcttatgc
     1201 ccgtgagttt ttgggtgaga aagccgctta tgttgctggc tggatgtact tcatcaactg
     1261 ggcgatgacg gggattgttg atattaccgc cgtcgctctg tatatgcatt actggggtgc
     1321 gtttggcggc gtgccgcagt gggtctttgc gctcgctgca cttaccatcg ttggcaccat
     1381 gaatatgatc ggtgtgaaat ggtttgcgga gatggagttc tggtttgcgc ttattaaagt
     1441 gctcgccatt gtgatctttt tggtcgtggg tacagtgttc ctcggtagtg gtcagccgct
     1501 ggatggcaac accactggct ttcatttaat caccgataat ggcggcttct tcccccacgg
     1561 tttgctgcct gcgctggtgt tgattcaggg cgtagtgttt gcttttgcct ccattgaaat
     1621 ggtgggtaca gctgccggag aatgtaaaga tccgcagacc atggtgccta aagccattaa
     1681 cagtgtgatt tggcgtattg gcctgtttta cgtcggctcc gtggtgttgc tggttatgct
     1741 attgccgtgg agcgcgtatc aggcggggca aagtccgttc gtgacgtttt tctctaaact
     1801 gggtgtgcca tatatcggca gcattatgaa cattgtggtg ctgaccgctg ccctctccag
     1861 cctgaactca ggtctgtact gcaccggacg tattctgcgc tcaatggcga tgggcggttc
     1921 cgcaccgagt tttatggcga aaatgagtcg tcagcatgtg ccgtatgccg ggattctagc
     1981 gacactggtt gtgtatgtcg tcggcgtatt cctcaactat ctggtgccgt cgcgcgtatt
     2041 tgagattgtg ttgaacttcg cgtcgctggg aatcatcgct tcatgggcgt ttatcatcgt
     2101 gtgccagatg cgcctgcgta aagcgattaa agaaggcaaa gcagcggatg tcagttttaa
     2161 actgcctggc gcgcccttca cttcctggct gacattactg tttttactga gtgtccttgt
     2221 gctgatggcg ttcgattacc cgaacgggac ttatactatc gcggcgctgc cgattatcgg
     2281 tattctgctg gttataggct ggtttggtgt gcgcaaacgc gttgctgaaa ttcacagcac
     2341 tgcgccagtc gtcgaggaag atgaagaaaa acaggaaatt gtgtttaagc ctgaaacggc
     2401 gagctaattg caaaattgcc cggctacgtg ccgggcaaat ttagattact gacgaaggtg
     2461 tgccgcatgc ggcgtgataa aagtcaggca agtctctccg ttttcaccct cgccacatct
     2521 attacagcgc aatacgaatc acatcgtccg gctgggtagc ttcctgctgt ttagtggatt
     2581 tttgtttcac actgacatac agcgttttgc catcggcaga cagcgccagg ctgtttggat
     2641 gagtcggcgt atcgaacgtt ttcaccactt tatagctttt cgcgtcaatc acactgactt
     2701 tacctgcctg acgatgcgtt acgtaggctt cattacgcgc tgggttaaac agcacagcca
     2761 gtgattctgg tgccgcaacc ttcgccagaa tattgccatt acgggtatcg accactaaca
     2821 cttctgcggc tttagaatcg gtgataaatg cacgctgcct ggcggtatca aggctaatgt
     2881 tgataaagaa gtgctctttg ccgtcatcca gcagcttttt acggctgagg attttattgt
     2941 cggcggtgtc gatggtaatc aattcgccgt cagcgttagt ggtgtaaaga cgtttgcctt
     3001 tgctatccag cgccagaccg gtactcattt taccggtgtt ctggatggcg gttttcagtt
     3061 tgatattctc gccatcaacg acccaaatca cgctctcttt accaatacca ctgatgtaaa
     3121 cggtgttcgt ggcatcatca gctaccagct cacgcggttg cagcgggcgc acctcttccg
     3181 tgcgcttacg atcatccagc accagacggc ctttcacctc gcccgttttg gcatctatcg
     3241 ccgtgaccgc gctgtttacg gtgttaccaa accacaacgt ctgagtcgtg ttattgatgg
     3301 tggcaccaaa cggcttgaga tcgttatgga tcgcctgcgt cacttccaga gtgaccggat
     3361 caagacgata aaccacgcca cctttatcca gtttgcggct ttgcgaagtg gcgagccaca
     3421 gtgcgttttc ttgctggcta taagccattt cgtaggcacc tttacctacc gctttacgca
     3481 gcatttcttc tgcggcctgc gtactgaatg atgaagcaac aagcaatgaa cctaacagta
     3541 atgaaccacg caggcgcgat gaaaacagat gacgtaaatg catgacgact ccctttgatg
     3601 aaccgatatt tttgctgctt cacaatgctt cgctaagcgt aatcatcgtt acgctcttgt
     3661 tataaatggg aatggtaatc attattttct caaatgtgga gcatttcttg ccgttacgac
     3721 tacaattttc tcaattactt aacgtatcgt gccgtttcgt tttccataaa tttacaattt
     3781 cattaccata acggcgtttt cccattctta acctggcttt tttcatgaag attttttccg
     3841 tccgacagac cgttttgccc gcactgcttg tcctttcccc cgttgttttt gccgctgatg
     3901 aacagactat gattgtcagt gccgcaccgc aggtggtttc agaactggat accccagcag
     3961 cagtaagcgt ggtggatggc gaggagatgc gcctggcaac accgcgcatt aacttgtccg
     4021 aatcactgac cggcgtgcct ggtttgcagg tacaaaaccg gcagaactat gcgcaagatt
     4081 tacagctgtc gattcgcgga tttggctccc gctccactta cggtattcgc ggtattcgcc
     4141 tgtatgtgga cggtattccc gccaccatgc ccgacgggca agggcaaaca tccaacatcg
     4201 atttaagcag tgtgcaaaat gtggaagtgc tgcgtggccc cttctctgcc ctgtatggca
     4261 acgcgtctgg tggggtaatg aatgtcacca cccagaccgg acaacagcca ccaaccattg
     4321 aagccagtag ttactacggc agttttggca gctggcgcta tgggctgaaa gcaacgggcg
     4381 caacgggaga cggcacacag cctggcgatg tcgattacac cgtctcaacc acgcgtttta
     4441 cgacccacgg ctatcgtgac catagtggcg cacagaaaaa tttagctaat gccaaactgg
     4501 gcgtacgcat tgatgaagcc agcaaattaa gtctgatttt caatagtgtg gatatcaaag
     4561 cagatgaccc aggtgggcta accaaagcag aatggaaggc taatccacaa caagcgcctc
     4621 gtgcagaaca gtacgacacg cgaaaaacca tcaagcaaac tcaggctggg ttgcgctatg
     4681 agcgtagcct gagcgcgcaa gatgatatga gtgtgatgat gtatgccgga gagcgagaaa
     4741 cgacccagta ccagtcaatc ccgatggccc cccaacttaa cccgtcacat gcgggcggcg
     4801 tgattaccct gcaacgccat taccagggga tagacagccg ctggacacac cgtggtgaac
     4861 tgggcgttcc ggtcacgttc actaccggcc tgaactacga aaacatgagt gaaaaccgca
     4921 agggctacaa taacttccgc ctgaatagcg gcatgccgga gtacgggcaa aaaggtgagt
     4981 tgcgtcgcga cgaacgcaat ctgatgtgga acatcgatcc ctatttacag acgcagtggc
     5041 agctgagcga aaaactgtcg ctggatgctg gcgtgcgcta cagctccgtg tggtttgatt
     5101 ccaacgacca ttacgttact ccgggtaacg gcgatgacag cggtgatgcc agttatcata
     5161 aatggctacc tgccggttcg ttaaaatatg caatgaccga tgcctggaat atctatctgg
     5221 cagccgggcg aggttttgaa acgccgacga ttaatgagct gtcttatcgt gctgatgggc
     5281 aaagcggtat gaactttggt ttaaaaccat ccaccaacga tacaattgag atcggcagta
     5341 aaacgcgtat tggtgatggg ctgcttagtc tcgcattgtt tcagaccgac actgatgatg
     5401 aaattgttgt cgatagcagt agcggtgggc gtacgactta caaaaatgcc ggaaagaccc
     5461 gtcgtcaagg cgctgaactg gcatgggatc aacgtttcgc aggagatttt cgcgtaaacg
     5521 cgtcctggac ctggcttgat gcgacctatc gcagcaatgt ttgcaatgaa caggattgta
     5581 acggtaatcg gatgccaggg atcgcccgta atatgggctt tgcgtcgata ggttatgtac
     5641 cggaagatgg ttggtatgca ggcacggaag cgcgttatat gggcgatatt atggcagatg
     5701 atgaaaatac ggcaaaagcg ccgtcttata ctctcgtcgg cttattcacc gggtataaat
     5761 acaattacca caatttaact gtggatttat ttggtcgtgt cgataattta ttcgataaag
     5821 aatacgttgg ttctgtcatt gtcaatgagt caaacgggcg atattacgaa cctgcgcccg
     5881 gacgaaatta tggtgtcggc atgaatattg cgtggagatt tgagtaatta ccttgatgcc
     5941 cggtatttgc cgggcattta ctttaacgat tgtattgctg gtataaaata gcaatatttt
     6001 gttgcataat ttcagcaaga caatgtctgc atccctcttt gtcttttgct tttaacgcag
     6061 cgagtaacag atgatagttt tctatatgct cccgtaattc cgctggatta atcgcttcat
     6121 agagataatg taaaccaggc cccatcctga cccacagttg ctcaatcatc tcacacagga
     6181 tgggcatatt tgagcgatga taaatcgcta agcgaaatag cctgtttaca ttaatgattt
     6241 gttccatgtc gcccttttct tgcgcctgct gtaatttctc gagcaattcc tggagttccg
     6301 ccaggtcttg cggggtgagg ttttcaacag ccagagcaac tgccattaat tccagttcgt
     6361 agcggatccg attgatttcg tctaattgac gtttccccac ttccggaact gtaaatgctt
     6421 gtgcaggtgc gacagaaagc gcattcaccg aaaccagacg taataatgct tcacgcacag
     6481 gtgtaatact catacctaat tgctccgcca gatttttagt aatcaggcgt gcgccaggtt
     6541 ttaatgcgcc aatactaagc tgatgtttca ggtcgttctc aacctgcaga gtcaaactga
     6601 catgtttcat ttttcccatt ccaggcattt taattccttc gcgtgttttc tgtccaaccc
     6661 tgcctgcacg gcagaaggag ttcacctggt gtattaaagt gaataatcat aaaccaccca
     6721 cctttgttgt tattagcagc agaacaatga cgtggttagt taattaaagc tgaaaataag
     6781 ctttttgcct ggatttttat ggcggcttac gccgccgttg aaactttaat catcacccgc
     6841 gacgcggatc accactttgc cgaagttttt acccttcagc aggccgataa acgtctgtgg
     6901 cgcattctct aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca
     6961 ttgccccatc tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa
     7021 cccttgcaag cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg
     7081 accgggtggt agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc
     7141 agatgtatta agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata
     7201 gatatcaata ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg
     7261 atcaagacaa acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc
     7321 ggcaaccccc accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac
     7381 aggacctgtc gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag
     7441 cagccccata taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt
     7501 ttgcggatga tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca
     7561 tccactgtag cccagcaccc agtcgccagg ctgataatca ggatgattcg actccacaac
     7621 acggctcacc gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg
     7681 ctcatcgctc atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa
     7741 cacctgtcct tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac
     7801 cggtgcgcca tgtggacgcg aggccagaac ccaacggcgg ttacgctgct tttgttgccc
     7861 cataatgctc tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cgcaggattg
     7921 gttttgtttg cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg
     7981 ttgcttcggg ctgttttcga tagtattgat aacatcatca ttattttatt gaggccagac
     8041 atgtccatcc gttttgcccg caaagccgac tgtgctgcca ttgcggaaat ttataaccac
     8101 gccgtgttgt atacggcggc tatctggaat gaccaaacgg tggatgcaga taatcgcatt
     8161 gcctggtttg aagcgcggac catagcaggt tatccggtgc tggtaagtga agaagatagc
     8221 gtagtgacgg gatatgcctc gtttggcgac tggcgtagtt tcgatggttt tcgccatacc
     8281 gtggaacatt cggtttatgt ccatcccgat catcagggca aaggtctggg gcgtaaattg
     8341 ttaagccgat tgattgatga agcgcgggat tgcgggaagc atgtcatggt cgccgggatc
     8401 gaatcgcaaa atcaggcctc gctgcatctg caccagtcgc tgggatttgt catcaccgca
     8461 caaatgccgc aggtgggcac taaatttggg cgctggctgg atctgacatt tatgcagttg
     8521 caactcgacg aacgcactga accggacgcg attggatgaa tcagtcgctc acccttgctt
     8581 ttttgattgc cgccggaatt ggtctggtag tccagaacac gctgatggtg cgcatcaccc
     8641 agacctcctc taccattctc atcgccatgt tgctgaactc actggtgggg attgtgctct
     8701 ttgtttctat tttatggttt aaacaaggca tggcggggtt tggcgaactg gtgtccagcg
     8761 tgcgctggtg gacacttata cccggcttgc tgggatcgtt ttttgtcttc gccagtatca
     8821 gcggatatca gaatgtggga gcggcgacaa ctattgcagt actggtcgcc agccagctaa
     8881 ttggcggtct gatgctggat atcttccgca gtcatggcgt accactacgg gcgttgttcg
     8941 gcccaatttg tggcgcaatt ttacttgtgg ttggcgcctg gttggtagcc agacgctcat
     9001 tttaatcaga ggatggtgcc gcctttggtt aactgttcgt ggcgcgcttc catatcttct
     9061 ttatgcttgc gaccgtgatg agcaatagcg gtgcgcaaac gctgctgctg ggtgtagcga
     9121 tcttcccgac ttaactccgc gtcatcgctc agggcgatta ataattcgtt catatgggca
     9181 atgacgctct cttcaatcgc ggcatccacg cgcgcgatga cttcatccag atgcgacata
     9241 ctttctcctt aaccacttgt gaaatttatc gattcatcca aatgatataa gtctgcgtgg
     9301 tgccgtcaag ccagtgagta agatttaaaa cagtgaaaac aacagcgcca ccgggaaact
     9361 catcggccat gttgccccca ccagaaaggc gcttaagaaa cggatgcgtt tcttatcgtg
     9421 agaaagaaac caggtgataa gaaatgcgat tgctgccata accgcataaa aaacaagcat
     9481 atgttgatag agagacatgt acgggcgtcc agccagatga ataaaacgcg cgcaaatatg
     9541 cgtcattttt tgacgtgcat caagtttttt caccagtttg agaaaaaatc atcaaacagc
     9601 aacttgccct tatccatctt cagaatgagt attattgcgg attttcttaa cccgtactat
     9661 acacataggg gcaatgataa aaggtggcaa aaatgaatgt ttccagtaga actgtagtac
     9721 tgataaatgt ctttgctgct gttggtttgt ttactcttat ctctatgaga tttggctggt
     9781 ttatttgatg tagtgatttt gcacgcagca cgtactgtca gagaaatcaa ctctctgaca
     9841 gtacgcaaga tcttctcata ctctccaggt ttacaacatt atccttaata tttacttctt
     9901 tttgctggca tctacattga aatgagtaga aaacatcccc ctactgtcgt ctatacaaac
     9961 agcttactga ttcaaacgg
//
LOCUS       DAZFRN010000169         9878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFRN010000169 DAZFRN010000000
VERSION     DAZFRN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.171"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            258..1235
                     /gene="glaH"
                     /locus_tag="ACHKUB_004219"
     CDS             258..1235
                     /gene="glaH"
                     /locus_tag="ACHKUB_004219"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO6657158.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1255..2523
                     /gene="lhgO"
                     /locus_tag="ACHKUB_004220"
     CDS             1255..2523
                     /gene="lhgO"
                     /locus_tag="ACHKUB_004220"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO6657159.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2546..3994
                     /gene="gabD"
                     /locus_tag="ACHKUB_004221"
     CDS             2546..3994
                     /gene="gabD"
                     /locus_tag="ACHKUB_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6657160.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPACQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILMDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4008..5288
                     /gene="gabT"
                     /locus_tag="ACHKUB_004222"
     CDS             4008..5288
                     /gene="gabT"
                     /locus_tag="ACHKUB_004222"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO6657161.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAGIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5599..6999
                     /gene="gabP"
                     /locus_tag="ACHKUB_004223"
     CDS             5599..6999
                     /gene="gabP"
                     /locus_tag="ACHKUB_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO6657162.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7020..7682
                     /gene="csiR"
                     /locus_tag="ACHKUB_004224"
     CDS             7020..7682
                     /gene="csiR"
                     /locus_tag="ACHKUB_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO6657163.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7683..8132)
                     /gene="kbp"
                     /locus_tag="ACHKUB_004225"
     CDS             complement(7683..8132)
                     /gene="kbp"
                     /locus_tag="ACHKUB_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO6657164.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8216..8374)
                     /gene="yqaE"
                     /locus_tag="ACHKUB_004226"
     CDS             complement(8216..8374)
                     /gene="yqaE"
                     /locus_tag="ACHKUB_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO6657165.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8557..8856
                     /gene="ygaV"
                     /locus_tag="ACHKUB_004227"
     CDS             8557..8856
                     /gene="ygaV"
                     /locus_tag="ACHKUB_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6657166.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8866..9390
                     /gene="ygaP"
                     /locus_tag="ACHKUB_004228"
     CDS             8866..9390
                     /gene="ygaP"
                     /locus_tag="ACHKUB_004228"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO6657167.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(9437..9841)
                     /gene="stpA"
                     /locus_tag="ACHKUB_004229"
     CDS             complement(9437..9841)
                     /gene="stpA"
                     /locus_tag="ACHKUB_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO6657168.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2268 a         2546 c         2697 g         2367 t
ORIGIN      
        1 tttactgttt tttatcgacc agataatctg ttctctaatg ttaactcccc ctaacctgtt
       61 gctttagtta ttcatttcct gtctcacttt gccttaatac cctacgttaa atgttactaa
      121 tttgttgctt ttgatcacaa taagaaaaca atatgtcgct tttgtgcgca tttttcagaa
      181 atgtagatat ttttagatta tggctacgaa atgagcatcg ccatgtcacc ctacatctca
      241 taagaggatc gcttctgatg aatgcactga ccgccgtaca caataacgct gtcgattcag
      301 gccaggacta tagcggattc actctcatcc cgtcggcgca atccccgcgt ctgctggaac
      361 tcaccttcac cgaacagacg accaaacagt ttctcgaaca ggttgccgaa tggcccgtgc
      421 aggcgctgga gtacaaatcg tttctgcgtt ttcgggtagg caaaattctt gacgatctgt
      481 gtgcgaatca gctgcaacca ttgctgttga agaccctgct aaaccgtgct gaaggtgcgc
      541 tgttgatcaa tgcggtgggt atcgatgatg tggcgcaggc agatgagatg gtgaagctgg
      601 caacggcggt agcgcatctg attggtcgct caaatttcga tgcgatgagc ggtcagtatt
      661 acgcgcgttt cgtggtgaaa aatgtcgata actcagacag ctatctgcgt cagccgcacc
      721 gcgtgatgga actgcacaac gacggcacgt atgtcgaaga gatcaccgat tacgtgctga
      781 tgatgaaaat cgatgagcaa aacatgcagg gtggaaattc gttgctgctg catctcgatg
      841 actgggaaca tctggaccac tatttccgcc atccactggc gcgtcgcccg atgcgctttg
      901 ccgcgccgcc gagcaaaaac gtcagcaaag atgttttcca tccagtgttc gacgttgatc
      961 aacagggtcg cccggtgatg cgctatatcg accagttcgt ccagccaaaa gattttgaag
     1021 aaggcgtgtg gttgagcgag ctttccgatg ccattgaaac cagcaaaggc attctttctg
     1081 tacccgttcc cgttggcaaa ttcctgttga taaacaacct gttctggctg cacggtcgcg
     1141 accgctttac tccgcacccg gatctgcgcc gtgaactgat gcgtcagcgt ggctatttcg
     1201 cttacgccac tcaccactac cagacgcatc agtaagcgca aaggaattga gcggatgtat
     1261 gattttgtga ttattggcgg cggcatcatc ggcatgtcga ccgccatgca actgattgat
     1321 gtttatccgg atgcccgcat tgcgttgctg gaaaaagagt ccggcccggc ctgtcaccag
     1381 acgggccaca acagcggcgt gatccatgcc ggggtctatt acacgcccgg cagcctgaag
     1441 gcgcagtttt gcctggcggg aaaccgcgcc accaaagcct tttgcgatca aaacgacatt
     1501 cgctatgaca actgcggcaa gatgctggtc gccacgtccg atctcgaaat ggaacggatg
     1561 cgcgcgttgt gggaacgcac ggcggcgaac ggtatcgagc gcgagtggtt aaacgccgag
     1621 gaactgcgcg agcgcgaacc gaatatcacc gggctcggcg gcatttttgt gccgtccagc
     1681 ggcattgtca gctatcgcga agtcacagcg gcaatggcaa aaatcttcca ggccagaggc
     1741 ggtgagatta tttataacgc cgaagtcagc gccctcagtg agcataaaaa cggcgtggtc
     1801 gtccgaaccc gtcagggcgg cgaatatgaa gcatcgacgc tgattagctg ttccgggctg
     1861 atggctgacc ggctggtgaa aatgctcggc ctcgaaccgg gttttattat ctgcccgttc
     1921 cgtggcgagt atttccgcct tgcgccggag cataaccaga ttgttaacca tctgatttac
     1981 cccattcccg acccggcaat gccatttttg ggcgtgcatc tcacccgaat gatcgatggc
     2041 agcgtcaccg tcgggccaaa cgcggtgctg gctttcaaac gcgaaggcta tcgcaagcgc
     2101 gacttctcat ttagcgacac gctggagatt ttgggctcgt cggggattcg ccgggtgctg
     2161 caaaaccatc tacgctcagg actgggcgag atgaaaaact cgctgtgcaa aagcggctat
     2221 ctgcgcctgg tgcaaaagta ttgtccccgg ctttcgttaa gcgatctcca gccctggccc
     2281 gccggtgtgc gggcgcaggc agtatcgccg gacggcaagc tgattgacga ttttctgttt
     2341 gtcaccaccc cacgcacgat ccacacctgc aatgcgccct ccccggcagc gacatcagca
     2401 attcctattg gtgcgcatat tgtcagcaag gtacaaacgc tgttggcaag ccagagtaac
     2461 cccggacgca cgctgcgagc ggcacgtagt gtggatgcct tacacgccgc atttaatcaa
     2521 taacctttga aaacaggacg taacaatgaa acttaacgac agtaacttat tccgccagca
     2581 ggcgttgatt aacggggaat ggctggacgc caacaatggt gaagtcatcg acgtcaccaa
     2641 tccggcgaac ggcgacaagc tgggtagcgt gccgaaaatg ggcgctgatg aaacccgcgc
     2701 cgctatcgac gccgccaacc gcgccctgcc cgcctggcgt gcgctcaccg ccaaagaacg
     2761 cgccaacatt ctgcgcaact ggttcaattt gatgatggag catcaggacg atttagcgcg
     2821 cctgatgacc ctcgaacagg gcaaaccact ggccgaagcg aaaggtgaaa tcagctacgc
     2881 cgcctctttt attgagtggt ttgccgaaga aggcaaacgc atttatggcg acaccattcc
     2941 cggtcatcag gccgataaac gcctgattgt tatcaagcag ccgattggcg ttaccgcagc
     3001 tatcacgccg tggaacttcc cggcggcgat gattacccgt aaagccggtc cggcgctggc
     3061 ggcaggctgc accatggtgc tgaagcccgc ctgtcagaca ccgttctctg cgctggcgct
     3121 ggcggagctg gcgattcgcg cgggcattcc ggctggcgtg tttaacgtgg tcaccggttc
     3181 ggcaggcgcg gtcggtaatg aactgaccag caacccgatg gtacgcaaac tgtcgtttac
     3241 cggttcgacc gaaattggcc gccagttaat ggaacagtgc gcgaaagaca tcaaaaaagt
     3301 gtcgctggag ctgggcggca acgcgccgtt tatcgtcttt gacgatgccg atctcgataa
     3361 agccgtggaa ggcgcgctgg cctcgaaatt ccgcaacgcc gggcaaacct gcgtctgcgc
     3421 caaccgttta tacgtgcagg acggcgtgta tgaccgcttt gccgaaaaat tgcagcaggc
     3481 ggtgagcaaa ctgcacatcg gcgacgggct ggataaaggc gtcaccatcg ggccgctgat
     3541 cgatgaaaaa gcggtagcaa aagtggaaga gcatattgcc gatgcgctgg agaaaggtgc
     3601 gcgtgtggtt tgcggcggta aagcggatga acgcggcggc aacttcttcc agccgaccat
     3661 tctgatggac gtgccggcca acgccaaagt gtcgaaagaa gagacgttcg gccccctcgc
     3721 cccgctgttc cgctttaaag atgaagccga tgtgattgcg caagccaatg acaccgagtt
     3781 tggccttgcc gcctatttct acgcccgtga tttaagccgc gtcttccgcg tgggcgaagc
     3841 gctggagtac ggcatcgtcg gcatcaatac cggcattatt tccaatgaag tggccccgtt
     3901 cggcggcatc aaagcctcgg gtctgggtcg tgaaggttcg aagtatggca tcgaagatta
     3961 cttagaaatc aaatatatgt gcatcggtct ttaactggag aatgcgaatg aacagcaata
     4021 aagagttaat gcagcgccgc agtcaggcga ttccccgtgg cgttgggcaa attcacccga
     4081 ttttcgctga ccgcgcggaa aactgccggg tgtgggacgt tgaaggccgt gagtatcttg
     4141 atttcgcggg cgggattgcg gtgctcaata ccgggcacct gcatccgaag gtggtggccg
     4201 cggtggaagc gcagttgaaa aaactgtcgc acacctgctt ccaggtgctg gcttacgagc
     4261 cgtatctgga gctgtgcgag attatgaatc agaaggtgcc gggcgatttc gccaagaaaa
     4321 cgctgctggt tacgaccggt tccgaagcgg tggaaaacgc ggtaaaaatc gcccgcgccg
     4381 ccaccaaacg tagcggcacc atcgctttta gcggcgcgta tcacgggcgc acgcattaca
     4441 cgctggcgct gaccggcaag gtgaatccgt actctgcggg catggggctg atgccgggtc
     4501 atgtttatcg cgcgctttat ccttgcccgc tgcacggcat aagcgaggat gacgctatcg
     4561 ccagcatcca ccggatcttc aaaaatgatg ccgcgccgga agatatcgcc gccatcgtga
     4621 ttgagccggt tcagggtgaa ggcggtttct acgccgcgtc gccagccttt atgcagcgtt
     4681 tacgcgcgct gtgtgacgag cacgggatca tgctgattgc cgatgaagtg cagagcggcg
     4741 cggggcgtac cggcacgctg tttgcgatgg agcagatggg cgttgcgccg gatctcacta
     4801 cctttgcgaa atcgatcgcg ggcggcttcc cgctggcggg cgtcaccggg cgcgcggaag
     4861 tgatggatgc cgtcgctcca ggcggtctgg gcggcaccta tgccggtaac ccgattgcct
     4921 gcgtggcggc gctggaagtg ttgaaggtgt ttgagcagga aaatctgctg caaaaagcca
     4981 acgatctggg gcagaagcta aaagacggat tgttggcgat agccgaaaaa cacccggaga
     5041 tcggcgacgt acgcgggctg ggagcgatga ttgccatcga gctgtttgaa gatggcgatc
     5101 acagcaaacc ggacgcaaaa ctcaccgccg ggatcgtggc acgcgcccgc gataagggcc
     5161 tgattcttct ctcctgcggc ccgtattaca acgtgctgcg catccttgta ccgctcacca
     5221 ttgaagacgc tcagatccgt cagggtctgg agatcatcag ccagtgtttt gctgaggcga
     5281 agcagtagct atgtccgttt gtaagagtta aacgttgcta agggaatgat aacgatgcag
     5341 taaaaaataa taacgatgag gcgatcgaca aagttaaaaa cgactgcgga ctgtcccctc
     5401 gctcctctgg ggagagggtt agggtgaggg gtaatcacgc ggctacaccc tcaccccggc
     5461 cctctcccta agggagaggg agaaaactct cccgctttcg tttttaagat tttgttgacc
     5521 gtctcaatta aacgcgtgac ccgacgcggg aaatgtcggg gcgctctccc aagtgacaca
     5581 ctttcgagag gattcaggat ggggcaatca tcgcaaccac atgagttagg cggcgggctg
     5641 aagtcacgcc acgtcaccat gttgtctatt gccggtgtta tcggcgcaag tctgtttgtc
     5701 ggttccagcg tcgccatcgc cgaagcgggc ccggcggtat tactggccta tctgttcgcc
     5761 ggattactgg tggttatgat tatgcgaatg ctggcggaaa tggcggttgc cacgcccgat
     5821 accggttcgt tttccaccta tgccgataaa gccattggcc gctgggcggg ttataccatc
     5881 ggctggctgt actggtggtt ttgggtactg gttatcccgc tggaagccaa catcgccgct
     5941 atgatcctgc actcgtgggt tccaggcatt cccatctggt tattttccct cgtcattacc
     6001 ctcgccttaa ctggcagtaa tttattaagc gttaaaaact acggcgaatt tgagttctgg
     6061 ctggcgctgt gcaaagtcat cgctatcctg gcctttattt tccttggtgc agtcgcaatt
     6121 agcggttttt acccttatgc cgaagtgagc gggatctcaa gattgtggga tagcggcggc
     6181 tttatgccca acggcttcgg tgcggtatta agcgcgatgt tgatcaccat gttctcgttt
     6241 atgggcgcag aaattgtcac cattgccgcc gcggaatccg acacgccgga aaaacatatt
     6301 gtccgcgcta ccaactcggt tatctggcgt atttctatct tctatttgtg ctctattttt
     6361 gtcgtagtgg cgttaatacc gtggaatatg ccggggctga aagccgttgg ttcttatcgc
     6421 tcggttctgg aattgctcaa tattccccat gcgaaattaa tcatggactg cgtgatatta
     6481 ctttccgtaa ccagctgtct gaactcggcg ctgtataccg cgtcaaggat gctctactcc
     6541 ttaagccgtc gcggtgatgc gcccgcgcta atgggcaaaa tcaaccgcag taaaaccccg
     6601 tatgtggcgg tgttactctc caccggagcg gcatttttaa cggtggtggt gaactattac
     6661 gcacctgcga aagtgtttaa attcctgata gacagctccg gcgctatcgc cctgctggtt
     6721 tatttagtca tcgccgtttc acagttgcgg atgcgcaaaa ttctgcgagc agaaggaagc
     6781 gaaattcgct tgcgcatgtg gctttatccc tggctcacct ggctggtcat cggctttatt
     6841 acctttgtgt tggtagtgat gctattccgt ccggcgcaac agttagaagt gatctccacc
     6901 ggcttattag cgatagggat tatctgtacc gtgccaatta tggctcgctg gaaaaagctg
     6961 gtattgtggc aaaaaacacc cgttcataat acgcgctgat aatgatcagg agtcacacca
     7021 tgaccattac gtctctggat ggctatcgct ggctgaagaa cgatattatt cgcggtaatt
     7081 ttcaaccgga tgaaaaatta cggatgagtt tgctgacatc gcgttatgca cttggcgttg
     7141 ggccgttgcg ggaagcactt tctcaactgg tggcggaacg gctggtcacg gtggtgaatc
     7201 aaaaagggta tcgggtggcg tctatgtcag agcaggagct gctcgatatt ttcgacgccc
     7261 gcgccaatat ggaagcgatg ctggtcagtc tggcgattgc ccgcggtggc gatgaatggg
     7321 aggcagacgt tctcgcaaaa gcgcatctgc tcagtaagct ggaagcctgt gacgccagcg
     7381 agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt tcatacagca attgtggcgg
     7441 gctgtggttc tcactatttg ctgcaaatgc gtgaacggtt gtttgatctg gcggcgcgct
     7501 atcgatttat ctggctgcgg cgaacggtgc tttcggtaga aatgctggag gataaacacg
     7561 atcagcacca gaccctgact gcggcggtac tggcgcgaga taccgcgcgc gccagtgagt
     7621 taatgcgcca gcatttactg acgccaattc ccattatcca gcaggcgatg gctggcaatt
     7681 aactactctt ccggaatacg caacacttgc cccggataaa ttttatccgg gctttttagc
     7741 atcggtttat tcgcttcgaa gattttattg tacagattag cgttaccgta gacctgtttg
     7801 gaaatggcac tcagagtgtc gccagactta acggtataaa actggctggc agtggctggt
     7861 gtcgccgttt tcacctgatc atcgacactg gcaataccgg aaatattccc aaccgcaaca
     7921 aggattttct ccttcgcctc ctgactcagg ccgtcaccag tgaccgtcgc tttgccgtcg
     7981 gcaatttgaa tgttcacttt atcggcatca ggtataccgg ttttgctcag atgctcctgc
     8041 accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa ccgcgtccca gagtttttct
     8101 ccggcatctt tcacaaaatt gaacagaccc atagctacct cattcgttaa cggaaacact
     8161 cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg ggggtgagca caggtttaat
     8221 cgcgggtttg cacccagaac gcgtgaatca aaccggggat atacccaagc agtgtcagca
     8281 gaatattaat aatgaacgcc caaccgaacc ctttaccgag cagcacgccg agcggcggca
     8341 gaatgatggt gatgacgatt ctccagaaac ccatatgtac tccctataag aaaattactc
     8401 attgtttaaa aagagatttt atctcttaag cgtagtaatt ttagcggagc ctgccagttt
     8461 cagcttacgc ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt
     8521 tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag
     8581 gccagtgccg aacaggcagc ggccttattg aaagcaatga gccatcctaa acgattgctg
     8641 attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc
     8701 ggactgagtg cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc
     8761 gacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc
     8821 attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc
     8881 atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt
     8941 gacgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg
     9001 ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag
     9061 gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa
     9121 atatttttac tcgaagatgg gattgatggc tggaaaagag ctggattgcc agtagcggta
     9181 aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata
     9241 ttaatcggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt
     9301 gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta
     9361 gataagatgc cgtggaacca acgaacttga gaagcgacgc cggacgcgcc ctagcagcga
     9421 catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct
     9481 gagcaattgg cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat
     9541 cggtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcgcgt ggtgcagcag
     9601 cagagctatt acctaataac tcttccgggt taattccgtc agccttcatc agctccagcc
     9661 aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac
     9721 gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga
     9781 attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca
     9841 taataaagaa aaccttttaa cgccaaaaca aaaaagta
//
LOCUS       DAZFRN010000170         9819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFRN010000170 DAZFRN010000000
VERSION     DAZFRN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.172"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..150)
                     /locus_tag="ACHKUB_004230"
     CDS             complement(<1..150)
                     /locus_tag="ACHKUB_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657169.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLN"
     gene            228..1322
                     /gene="ddlA"
                     /locus_tag="ACHKUB_004231"
     CDS             228..1322
                     /gene="ddlA"
                     /locus_tag="ACHKUB_004231"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6657170.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1346..1558)
                     /gene="yaiZ"
                     /locus_tag="ACHKUB_004232"
     CDS             complement(1346..1558)
                     /gene="yaiZ"
                     /locus_tag="ACHKUB_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO6657171.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1818..2126
                     /gene="yaiY"
                     /locus_tag="ACHKUB_004233"
     CDS             1818..2126
                     /gene="yaiY"
                     /locus_tag="ACHKUB_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO6657172.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2185..3279)
                     /gene="yaiW"
                     /locus_tag="ACHKUB_004234"
     CDS             complement(2185..3279)
                     /gene="yaiW"
                     /locus_tag="ACHKUB_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO6657173.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3292..4499)
                     /gene="sbmA"
                     /locus_tag="ACHKUB_004235"
                     /pseudo
     CDS             complement(3292..4499)
                     /gene="sbmA"
                     /locus_tag="ACHKUB_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            4851..6008
                     /gene="ampH"
                     /locus_tag="ACHKUB_004236"
     CDS             4851..6008
                     /gene="ampH"
                     /locus_tag="ACHKUB_004236"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO6657174.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6009..6632)
                     /gene="iprA"
                     /locus_tag="ACHKUB_004237"
     CDS             complement(6009..6632)
                     /gene="iprA"
                     /locus_tag="ACHKUB_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO6657175.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6720..9698)
                     /locus_tag="ACHKUB_004238"
     CDS             complement(6720..9698)
                     /locus_tag="ACHKUB_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6657176.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNHDFTDGTTN
                     QLNISNSLIHGSITSMLPMGESSDGHYVYQFSEYGTDRVIDDNWHDGDVFTLNIANST
                     IDDDYEGLYFTDSYLDGDVTKYTNETFHTPAGEGEEYAGLFANGGVGLGLAVNLDVES
                     NINISNNSRVAGISLTQGNTVNNTYTTESHTWDNNISVTDSTVTSGSVTTLEDSGFYG
                     NSAEPSDYSGNGEANDVALYFSDSAASNYSMKNNVYFSNSTLLGDVVFASTFNANFYP
                     QGHDSNADGVLDTNGGWADDSLNVDELNITLDNGSKWVGSATTSANVDDESTASTDWY
                     DVTGNSLYPGVVTEANAWGRTIDDQVFQSGVFNVTLNNGSEWNTVNASNIDTLAVNNG
                     SQVNVSESSLISDSIALTNGSSLNIGDEGYVGTDHLTVDSYSTVALSSDDNSSIYNGY
                     YTPSLLANTITVTNGGLLDINVDQFDDNPFQTDTLELTSGNIADHNGNVVSGVFDIHS
                     SDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDMNNGDELDNSSVDNVV
                     AATGNYKVRIDNATGAGAIADYKDKEIIYVNDVNTNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
BASE COUNT         2571 a         2425 c         2303 g         2520 t
ORIGIN      
        1 attaagagaa aaaaactcac cgcatggcca tttaagccag gaataaatta gcttcccgtt
       61 catcattttt gtgatcttgc gcacatcttt gagataacgc actttcttca ccagtacgtt
      121 gcacgctttg tcagcagaag attgcaacaa tctttcagaa ggccgcgaat cccccgtttt
      181 gtcgtggtaa tgtatgcact ttgtgttaga cgggataggt ttttaagatg gaaaaactgc
      241 gggtaggaat cgtttttggt ggtaaatcag cggaacatga agtgtctctg caatcggcaa
      301 aaaacattgt cgatgccatt gataaaagtc gcttcgacgt tgtgctgctg ggcattgata
      361 aacaagggca atggcacgtc agcgatgcca gcaattatct gctaaatgca gacgatcctg
      421 cccatattgc gttgcgccct tcggcgacca gccttgcgca ggtgccaggt aaacatgagc
      481 atcagcttat cgacgcgcaa aacggtcagc cgttgccgac ggtggatgtc attttcccga
      541 ttgtccacgg tacgctgggc gaagatggtt ccttgcaggg aatgctgcgg gtcgccaatt
      601 taccgtttgt aggttctgat gttctggctt cagcagcctg tatggataaa gatgtcacca
      661 aacgtctgct acgcgatgcc gggctgaaca ttgcgccatt tattaccctg acgcgcgcta
      721 atcgtcacaa catcagtttt gccgaagtgg agtctaaact ggggttacca ctgtttgtaa
      781 aaccggctaa tcagggctct tctgttggtg tcagcaaagt aaccagtgaa gaacagtacg
      841 caattgccgt cgatctggcg ttcgagttcg atcataaagt gatcgttgag caagggatca
      901 aaggtcgtga gatcgaatgc gcagttctgg gcaacgacaa tccgcaagcc agcacctgtg
      961 gcgagatcgt actcaccagc gatttctatg cctacgacac caagtacatt gacgaagatg
     1021 gcgcgaaagt ggtagttccg gcagccattg caccagagat caacgataag atccgggcga
     1081 ttgccgttca ggcttatcag acgttgggat gcgcaggcat ggcgcgtgta gacgtgtttt
     1141 taaccccaga gaacgaagtg gtgatcaacg agatcaacac actgcctggc ttcaccaaca
     1201 tcagtatgta tccgaagctg tggcaagcca gcggtctggg ttacaccgat ctgatcacac
     1261 gtttgattga gctggcgctg gagcgtcacg ctgcggataa cgcattgaaa accacaatgt
     1321 aatattttaa cgcccgataa tattcttatt gggcgttttc agtttcttca tcgcgacgac
     1381 gaataatcaa ccccgccagc cagaaactaa tcacccacgt caccagaccg acggcatagg
     1441 tctgccaacc ttttgcttca aatcccagta accccaccac gccattaaga atgaatataa
     1501 ggccaatggc gaacgcatag tagtgccagt cacggcggat ttttacaggc agattcatgg
     1561 cagctccgtt aacgaaaaac aacatccttg catattctgt aacaaccgtc tgtggtgtag
     1621 acaacatttt cgtaattgtt aataaattta acttaaggtt gccattttgt gactcacaac
     1681 gaagattcac gcttcgggaa tattcccaac atcaatttac catgattctg atattgacaa
     1741 tctaaatgac ctgtcagact tgccataagc cgctggtatc acaccagaag agaagcagat
     1801 gacttccgga ggtttttatg gctgatttca ccctgtcaaa atcgctgttt agcggaaaat
     1861 atcgcaatgc ctcttcaacg cctggcaaca ttgcctatgc gttgtttgtg ctgttttgct
     1921 tttgggctgg ggcgcaattg ctgaacctgt tagtgcatgc gcccggcgtc tatgagcgtt
     1981 taatgcaggt ccaggaaaca ggtcgcccac gggtggaaat tggtttaggt gtcggcacca
     2041 ttttcgggct gatcccgttt ttagtaggct gcctcatttt tgcagtggtg gcgctatggc
     2101 tgcactggcg acatcgccgc cagtaagatt tacgttgcca gatacggcct aaaacgcctt
     2161 atctggcatg acattcctgt ttgatcactg tttcatacaa cgcgcccgcc gttcgtctac
     2221 gcgcttcgca aaccaggccg tagtcaggtt gcgggtgatt ttcgggcttt ccagttgaat
     2281 gccaggcaac atttcgcggg gtagtgattt acccgttttc gcttcggcaa gttgataaac
     2341 tttcttgtac agcgccgtct cctcaaaaga gaagctgtca cctttctcta actgacggcg
     2401 aatttcgctg tcgctcatcc ccagttttcc cgccagtttg cgcgttgcca gttcggtttt
     2461 cccgggttct ttactgtcgt aacgaattaa atcaccgtcc agtgccagct tcacgccgct
     2521 ggccttactg actgcgttct gaaacgcggc attacggctg gcgtaccagc cagcgttaaa
     2581 atcagcaaag cggtatatcg gtgcgctata gctggcggga taattcagta aatggtaagt
     2641 accaaaccac aacccgccgc ggcgactgaa gacttcctga cgaactgtac cgtccatttt
     2701 ccacggatac cctttggtat gctgttcggc aaaagcaatg ctgacctgca tcggcccacc
     2761 ggtgcgcaca ggattaagcg aaccaaacag cgtctgcccc attggtacca tgctgatcag
     2821 atcgtcaaaa atcgcgctca attgcttttc tgtacgcacc gaatctaacc gttcgctata
     2881 acttttgccg tttggcgatt tgatttttag cgcggtatga accagaaaag caggaatgtg
     2941 catccgttca gcacgacggt caatctcttg ccaggcgatc ttgcttaagc ccggaacggc
     3001 cggatctgcc tgataatttg actcctgctg cgccaccgcc agcaccgagc agacgttttc
     3061 cagcgtcggt gccagcccct gactttcaaa ggtggtggcg agatctttcg cccaggcatc
     3121 gcggtctttc acgctcgcag gcatcttctg ccgcacgacg ctcgccacat ctatcgcttt
     3181 ttcgcccttt ttcaacggtt gcggagcctg actactacac cccgctaaaa ccagaaccgc
     3241 caacaatgac agcgaagata gaggattgac gcgcgacatt acttctcctt tttagctcaa
     3301 ggtatgggtt acttcctgaa ttttgtcacc atccagttca tgttcaaagc tgcgcagacg
     3361 tttgtagata gacatcaggt ccatgagtta atcaggtact ggaaagcacc gcgaacctga
     3421 ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc aacaattgac
     3481 ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat gcgggcgatg
     3541 ttgaaataca tatagtgaaa atagaggcgg aaatagtttt tccgcacggc gctaaacagc
     3601 tcgcgtaccg taggcggcgt cgcacgcgtg gcatcgtctt caccataaac cagctcttta
     3661 cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt gatccctacc
     3721 actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac cagaccatac
     3781 ggaatgtgcc cgataatcgg cagctccggc acatgcgtgg agagcgttac cagcaccggc
     3841 aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt ctccagcgtt
     3901 gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg ccccttcgat atgacgcagt
     3961 tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa cacgtagtga
     4021 ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat ccccagaaag
     4081 acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact tagcgcggtt
     4141 tgaatcagat catagaacgg tgcataccag gcgttgacgg cgaccccgac ttccaccaaa
     4201 aaccaggtga cgaagatgat cagtgcagta ccgagtattg accagtattg ccaacggtgc
     4261 gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta gtaagcgtaa
     4321 aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg cccggaagcg
     4381 ccggtgatac gcgccaccca gtcaccccca ccggcttgcc agaagataac ggcaatcaat
     4441 gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga cttaaacata
     4501 tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg catgaccgct
     4561 acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc ttagttcgcc
     4621 agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt tacataagcg
     4681 gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag atcgtgttta
     4741 cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa tccatcaatg
     4801 agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg ttgaaacgta
     4861 gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat gcggcgcagc
     4921 cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat catatttttt
     4981 acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag cgcgtctttc
     5041 gtagttatgg cgaaacgcga cctggtaata acgttcgccc gcagctggat tcggtcgtgc
     5101 gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg ctcgaccagg
     5161 ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca cgcgtgccga
     5221 cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc gccctgcccc
     5281 gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg cgcgaacaac
     5341 gctggaaata cctttctacg gcgaagctaa aagccgcgcc aggcagtcag gcggcgtact
     5401 ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc aagccttata
     5461 cccagttgtt tgaagagcag attacccgtc cattggggat gaaagacacc acctacaccc
     5521 cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt ccgtgcaata
     5581 acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat atgatgcgct
     5641 ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca gatcgcatgc
     5701 agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat gtccccggca
     5761 aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt cgtccgggga
     5821 ttattcagaa gacaggaggt ggtggcggtt tcattaccta tatggcgatg atcccgcaga
     5881 agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt aaaaatatga
     5941 gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg gttatccccg
     6001 catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc tttattcatt
     6061 tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga acgcgatatg
     6121 cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc ttcattccag
     6181 tcaatcattg ataataatgt tgcgcggatt tgttcgtagg aattatgccc aatgagctgc
     6241 acgtcgcgta attccagaat tctattttgc caggctaacc agtaaaaagc gtctcgccag
     6301 agttgatttt gttcaataag cgtaatggtt tgtttggctg gtagatgata tcccgtacaa
     6361 tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc agccagcccc
     6421 ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg cagagagata
     6481 acgccctcta aaataacgaa agtatcttcg ctattgacat cactggaaaa tataacttgc
     6541 ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc gagcttacca
     6601 aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt aatgcccgtc
     6661 aaatccttga cgggcattat ttagattaaa ttaccagaat ttcttcggag tgaagaatat
     6721 taccaggtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc accaccaccg
     6781 aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg agtacccaga
     6841 ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt cacatcgtta
     6901 tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag agcctgatct
     6961 tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat gctgtcgtaa
     7021 gactgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga ctggaacaga
     7081 ccagaaacgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa gtcgtaaccg
     7141 gctttcaaac cgaagcccca cgcgtcagag ttggtgctac cgtcaacgta agtaccgttg
     7201 ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaagctacc atcaacaaag
     7261 acgttgttcg cgaagtgagc agaagagtag atgtaggcag tctggctgtc ttgatccacc
     7321 tgaccagaac ggtcattcat gtcaccttta gcgaagcctg cagccgcacc gacgatccac
     7381 ttagcgttgt taccgtcaat tttggtatca acaccgacca tgatgccgtt aacatcctga
     7441 tcatagttga tggtgccgtt gtcgccgttg aagttaccac cgaagtagct tacccatgcg
     7501 ccgccgttat cagccaggcc atgacgagag ttggtcagac gagtaccaac ggtgtcttgt
     7561 tccaggttcc agatattggt gttcgcagac gggatgctca gcgccatgtt agcgtagtcg
     7621 gtcagctcca tctgttgcag aacaacggtg ttaccgcgct gttcagcctg ataggtgtat
     7681 gcacccaggt cagctttgtt agcagcagag aaggtcgcgt tggtgttgac gtcgtttacg
     7741 tagataattt ctttatcttt gtaatcagcg atagcgccag cgcctgttgc gttgtcgata
     7801 cgaactttgt agttaccggt tgcagcaaca acgttgtcaa cagagctgtt atccagttcg
     7861 tcgccgttgt tcatgtcacc gttaccgttg atagtcagat gaccatcaga gttcatagca
     7921 acaataccgt agccgtagtt agacttggaa gtatcccagg tacggtcgtt caccaaatca
     7981 gcgttcagaa cataatcgct gctgtggata tcgaatacac cagacactac gttgccgtta
     8041 tggtcagcga tgttgccgct ggtcagttcc aaagtatcag tctggaacgg attatcatcg
     8101 aactggtcta cattaatatc cagtaaacca ccgttagtca cagttattgt atttgctaac
     8161 agcgacggag tgtaataacc attatagata cttgaattgt catcactaga cagcgctaca
     8221 gtactgtagc tatcaactgt cagatgatca gttccaacat aaccttcatc tccaatgttc
     8281 aaagaagaac cattggtcag agcgatgcta tcagaaatca agctagattc agacacatta
     8341 acctgagagc cattattcac agctaaagta tcaatgtttg aagcgttaac ggtattccat
     8401 tcagaaccat tattcagagt gacgttaaac acaccgctct ggaatacttg atcatcaatc
     8461 gtacgacccc aagcatttgc ttctgttaca acgcccggat acaggctatt acctgtaaca
     8521 tcataccagt cagtcgaagc agtagattca tcatcaacat ttgctgatgt agtagctgag
     8581 cctacccact tagaaccatt atccagagtg atattcagtt catcaacatt aagtgagtca
     8641 tcagcccagc caccattggt gtccagtacg ccatcggcat tactgtcatg accttgagga
     8701 tagaagtttg cgttgaaagt acttgcgaaa acaacatcac ccagcagagt agaattgctg
     8761 aaataaacat tgtttttcat ggagtaattt gaagccgcac tatcactgaa atacagagct
     8821 acatcattag cttcgccgtt accagagtaa tcactaggtt ccgcagagtt accatagaaa
     8881 ccactgtctt ccagagtagt tacagaacct gaagtcactg tggaatcggt aactgagata
     8941 ttgttatccc aagtatgaga ttcagtagtg taagtattgt ttacggtatt accctgagtc
     9001 aggctgatac cagcaacgcg ggagttgtta gaaatattga tgttactttc gacatcaaga
     9061 ttcactgcca gcccaaggcc aacaccaccg tttgcaaaca aaccggcgta ttcttcacct
     9121 tcaccagctg gggtatggaa ggtttcgtta gtatatttgg taacgtcacc gtccagatat
     9181 gaatcagtga agtataaacc ttcataatcg tcatcaattg tagaattagc aatatttaaa
     9241 gtgaatacat caccatcatg ccaattatca tcaattacac gatcagtacc gtattcagag
     9301 aactgataga cataatgacc atcactgctt tcacccatag gcagcattga agtgattgaa
     9361 ccatgaatca gggagttgct gatgttcaac tggtttgttg ttccatcagt aaagtcatga
     9421 ttcaggtaat atgtagagat agtcccgtta acagtgtcac cattaaaggt ggtatcgaaa
     9481 tcaccgtttg tgttgttata gtagacaaag ccactataat tcacatagtt accatctaca
     9541 gtacccggtg catccatatt gctccagtca agagcggtag ttggattgtc gtaataagtc
     9601 caggtgtcat aagtagttgc attagcctga gaggtgatag ccagagtgca agccaatgct
     9661 aattgtgata ctacaagttt ctttttccag gagtgcattt gtcatccctc ctcagggacg
     9721 taataagttg atccatcaat tatcaatgca tagaaaacat attatgtttc gcgctaacga
     9781 ttatgctgcg ttacagacga atggttcaat tattctttc
//
LOCUS       DAZFRN010000171         9791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFRN010000171 DAZFRN010000000
VERSION     DAZFRN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.173"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHKUB_004239"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHKUB_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO6657177.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHKUB_004240"
     CDS             complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHKUB_004240"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO6657178.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHKUB_004241"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHKUB_004241"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO6657179.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="ACHKUB_004242"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="ACHKUB_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO6657180.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="ACHKUB_004243"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="ACHKUB_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO6657181.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="ACHKUB_004244"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="ACHKUB_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO6657182.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6259..7419
                     /gene="pheA"
                     /locus_tag="ACHKUB_004245"
     CDS             6259..7419
                     /gene="pheA"
                     /locus_tag="ACHKUB_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO6657183.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHKUB_004246"
     CDS             complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHKUB_004246"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO6657184.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHKUB_004247"
     CDS             complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHKUB_004247"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO6657185.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
BASE COUNT         2494 a         2610 c         2299 g         2388 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctggatagc acgtttcaaa
      241 ggacgtgcac catagactgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcaata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcaaat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgacca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc agacgagaca ccgagtgttt ctccataaac
      781 tcggacatat cgatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct cagttttccc cacacccgtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccaaccc cgcacggcta cggcgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaatgac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacg
     1981 cggcggcctt tcaatccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag cggatgttaa taaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gataaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgaccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgctcaac acccacgttc
     2941 gccagacgct gccgggcaag ctgataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgcac ttgctttagc gtctactgcc ataaacgcct cgcgaacctc cacccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaaccgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc gggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagaag agctacaggc cgcaacacct tttggtagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gctcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgaa gcgcctggcg gtcaaactta cgcagcatgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgccggttt gcccatcgga tgcaccgcca tgtgagtacg tttggtcggg tggcggctga
     3961 tgggttcatc tactgtaccg ccagcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgtggta
     4141 catcggcaat gggcggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gacgatgata tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cgttgatggc aacctgctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgtttaccag cacgcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcgcaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgttg accgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccaaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggaa tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa tacaaaacgg cagcccttga gctgccgttt ttttattctg ttagttgtga
     5461 aactgaagcg atttagtcac tatcgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattccca tcacacattt tgacatcagg agcggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     6241 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6301 agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaatt ggccgtcgag
     6361 gtaggaaaag ccaaactgct ctcgcatcgc ccggtacgag atattgatcg tgaacgcgat
     6421 ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6481 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccagcaa
     6541 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     6601 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     6661 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6721 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     6781 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     6841 tccggcacga ctgatttatc caccattaat acggtctaca gccatccgca gccattccag
     6901 caatgcagca aattccttaa tcgttatcct cactggaaga ttgaatatac cgaaagtacg
     6961 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc attgggaagc
     7021 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcaa
     7081 caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtgtc tgaccaggtt
     7141 ccggcgaaaa cgacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7201 ttgctggtgc tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac
     7261 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa
     7321 atgcaaaaag cattgaaaga gttaggggaa attacccgtt caatgaaggt attgggctgt
     7381 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     7441 gaatcatccg gcactggatt attactggcg gttgtcattc gcctgacgca ataacacgcg
     7501 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7561 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7621 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt cggcataaag
     7681 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7741 agagagcgcc agaagttgct caagctgaac attttcttct gccagatgca gcccataagc
     7801 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     7861 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     7921 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     7981 gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     8041 tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8101 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8161 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8221 cgagagggtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8281 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8341 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8401 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8461 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tagctaataa
     8521 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8581 cataataaac ctcttaagcc acgcgagccg tcagcagccc gttcagatcc tgatgaattt
     8641 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8701 atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga
     8761 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8821 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     8881 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     8941 agttcggcgc tttaccaccg cgcaggatga catgcccgtc cggattcccc tgagtttgta
     9001 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9061 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9121 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9181 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9241 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9301 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9361 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9421 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9481 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttt
     9541 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
     9601 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
     9661 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9721 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg
     9781 caatttagtc g
//
LOCUS       DAZFRN010000172         9640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFRN010000172 DAZFRN010000000
VERSION     DAZFRN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.174"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(37..615)
                     /gene="mocA"
                     /locus_tag="ACHKUB_004248"
     CDS             complement(37..615)
                     /gene="mocA"
                     /locus_tag="ACHKUB_004248"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO6657186.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMV
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            718..1488
                     /gene="yqeC"
                     /locus_tag="ACHKUB_004249"
     CDS             718..1488
                     /gene="yqeC"
                     /locus_tag="ACHKUB_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO6657187.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1536..3161
                     /gene="yqeB"
                     /locus_tag="ACHKUB_004250"
     CDS             1536..3161
                     /gene="yqeB"
                     /locus_tag="ACHKUB_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO6657188.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3202..4134)
                     /gene="arcC"
                     /locus_tag="ACHKUB_004251"
     CDS             complement(3202..4134)
                     /gene="arcC"
                     /locus_tag="ACHKUB_004251"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6657189.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTKQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4182..5567)
                     /gene="hyuA"
                     /locus_tag="ACHKUB_004252"
     CDS             complement(4182..5567)
                     /gene="hyuA"
                     /locus_tag="ACHKUB_004252"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO6657190.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEIYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5620..6831)
                     /gene="ygeY"
                     /locus_tag="ACHKUB_004253"
     CDS             complement(5620..6831)
                     /gene="ygeY"
                     /locus_tag="ACHKUB_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO6657191.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6889..8085)
                     /gene="dpaL"
                     /locus_tag="ACHKUB_004254"
     CDS             complement(6889..8085)
                     /gene="dpaL"
                     /locus_tag="ACHKUB_004254"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO6657192.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8143..9330)
                     /gene="ygeW"
                     /locus_tag="ACHKUB_004255"
     CDS             complement(8143..9330)
                     /gene="ygeW"
                     /locus_tag="ACHKUB_004255"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO6657193.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2248 a         2343 c         2522 g         2527 t
ORIGIN      
        1 attaaatatt tgaacgcaat cgtttaacta cattttctaa attttagtat accttttttg
       61 cgctgtaata aagtcgtccg gagtatcaat atctaaaatt atttctgcat tttctatctc
      121 gacggaataa tgagctacca taaggagtgc ctgacgcata ttggtgacat tgggtcgttg
      181 gattgcctgc atcaagcatg gttttgacac taaaatcgga tggccgggga tgccattatg
      241 gagtggcagt attgcgccat catttcgtaa cgaccagatt tttctaaaaa tatcgatggt
      301 gagggttggc atgtcaccgt gggtgagaaa acaatgttct gtttgcactg cgggtactgc
      361 ggcctttact gatgttagta aaccctgcgc ataatctggg ttgtggataa tagtgatatt
      421 gctctggttc gcataacgtt cgtgcagttc attaccacga tagccggtga ctaaaataat
      481 tcggctacaa aactgcaacg catttttgat acttgtatca agaattgtcc cctgttgcca
      541 gggtaacatc attttccatt gccccattct tgatgataat ccggcagcgg taattataca
      601 gtcgatggct gacatagtgg ttccattatt tataggggct aataacgcct gataattgcg
      661 ttacttcctg atggattaag ttggggataa tttatcccag agaggtcata aagactcgtg
      721 aaaagtatag ttgacccatc ggcattattc attgatttag gtgcgcagaa acgtcccact
      781 gtgatttctg ttgttggtgc tggggggaaa accagcttgc ttttttggct ggcagaactg
      841 ctccaggcta gcggcaggcg tgtgttaatc acgacgacaa cacatatgtt tatgccaacg
      901 tctcactggc ccgtggtttt ctgtcgtgat cccgccatgc ttcctcatgc gtctcttaca
      961 tcccccattt cattttgttt tcacagctgg aaagcgaacc agggaaaagt gcagggattt
     1021 acgccagaag cgattgatgc actggtgcaa cgaccagagt gtgacgtaat tctcattgaa
     1081 gcagatggct cgcgtggaat gccgttaaaa gcgcctgatg agcacgaacc ttgcataccc
     1141 aaaagcagtt gctgcgtgat tgctgtgatg ggaggacata ctttgggcgc gaaagtgagc
     1201 acagaaaatg tccatcgctg gtcgcagttt gctgatatta ctgggttaac acctgatgca
     1261 accttgcaac tgagcgatct cgttgcgctg gttcgccacc ctcagggggc gtttaaaaac
     1321 gtaccgcaag gttgtcggcg agtctggttc attaaccgtt tttctcaatg tgagaatgcg
     1381 attgcacaaa gcgagctcct tcaaccgctg caacaacacg acgtagaggc aatctggctg
     1441 ggcgatatac aagaacatcc tgcaatcgcg cgcagatttg tgaattagcg gcccgggtat
     1501 tccgggttac tgaaaggata tccattgagg ttggtatgaa tattttcaca gaggctgcaa
     1561 aactcgaaga gcaaaattgt ccgtttgcta tggcgcaaat tgttgatagc cgaggctcga
     1621 ctccccgcca ttctgcacaa atgttagtgc gcgccgatgg ctctatcgtc ggtacaattg
     1681 gtggcggaat ggttgagcgg aaggtgattg aagagtcgct tcaggcattg caggaacgta
     1741 agccgcgatt attccatgga cgtatggctc gtaacggtgc ggatgctgtc gggtcagatt
     1801 gtggaggtgc gatgtcagtg tttatcagcg tccatggtat gcgtccacgt ctggtgttga
     1861 ttggcgcggg gcatgtcaac cgggcgatag cccagagtgc ggcgctatta ggatttgata
     1921 ttgccgttgc cgatatttat cgcgaaagcc tcaatcctga actattccct ccatcaacca
     1981 cgcttctcca tgctgagtcg tttggtgcag cagtggaagc actggatatt cgccctgata
     2041 attttgtcct gattgccacg aataatcagg atcgtgaagc cctcgacaaa ctcattgaac
     2101 agcccattgc atggttgggg ttgctggcaa gtcgccgcaa ggttcagctt ttcctgcgtc
     2161 aattgcgtga gaaaggcgtg gctgaagaac atattgcccg tttacatgcg cccgttggtt
     2221 acaacatagg tgcggaaacg ccgcaggaga tcgccatcag cgtgctggca gaaatattac
     2281 aggtgaaaaa taacgcgccg ggtgggctga tgatgaaacc ttctcatcct tccggacacc
     2341 agctggtggt gattcgcggt gcgggtgata tcgccagtgg tgtggcgcta cgtctgtatc
     2401 atgcgggttt taaagtgatc atgctggaag tggaaaaacc gacagtgatt cgttgtaccg
     2461 tggcgtttgc ccaggccgtg ttcgatggcg aaatgacggt cgaaggcgtc actgctcgcc
     2521 tggcaaccag ctctgcggaa gcgatgaaac ttaccgaacg cggattcatc cctgtgatgg
     2581 tagatcccgc ctgttcattg cttgatgaac tgaaaccgct ttgcgtggtg gacgctattc
     2641 tggcgaaaca gaatttggga acgcgggcag atatggcacc agtaacaatc gcgcttgggc
     2701 cgggctttac tgcagggaag gattgtcatg cggtaattga aacaaatcgc gggcactggc
     2761 tcggtcaggt gatttactct ggttgtgcgc aggagaatac cggtgttcct ggcaatatta
     2821 tggggcatac cacccgacgg gtgatccgtg ctcctgctgc aggcattatg cgatccaacg
     2881 tgaaattagg cgatctggtg aaagagggcg atgtgattgc ctggattggt gagcatgaaa
     2941 ttaaagcacc gttgacgggg atggtgcgtg gcttgttgaa cgacggcctg gcagtggtcg
     3001 gtggttttaa aattggtgat atcgatcctc gtggtgaaac ggctgatttc accagcgttt
     3061 ctgataaagc ccgggcgatt ggcggcggcg tacttgaggc gttaatgatg ttgatgcatc
     3121 agggcgtgaa agcgacaaaa gaagtgctgg aagtggctta aatgaaaatg gccggatggt
     3181 attacccacc cggccttaaa attactgaca aatcaccgtt ccggttttgc cttcaatccc
     3241 ctctttcgct ttgctcaata cggtaatcag tgcttcacga cctgcgcgag agcgagcaaa
     3301 cgaggcggca gcttccactt taggcagcat agaacctttc gcaaaatgac cttcttcaat
     3361 aaagcgttca gcatcactta atgacaggcg atcgagccac tgttcattct ctttaccgaa
     3421 gttaatggcc actttttcta ccgcagtcag aatgatcagc atatcggcat caatcatttc
     3481 tgctaaacgg gcgctggccc agtctttatc gataaccgcg ctggcaccac gcagatggtt
     3541 accttcacga ataacaggga taccaccacc gccaacggtg atcaccacct ggcctgcatc
     3601 taccagagct ttaaccgttt ctttttcaat gatatcaacc ggttttggcg aggcaacaac
     3661 acggcgatag ccgcgaccag catcttcttt cagagtgtaa ccctgttttg tcagttgctc
     3721 tgcttcttgc ttggtaaaga acgaaccgat cggcttggtc gggttgagga atgccggatc
     3781 gttagcgtcg acttcgacct gagtaaccag cgttgctacc ggtttattaa tgccccgaga
     3841 aagcagttct tctcttaagg cgttttgtag atcgtaacca atataaccct ggcttaaggc
     3901 aacgcagaca gacatcggca gcattggcga gtgcgcttca gtttttgcag cagcttcaaa
     3961 ggcctggtta atcatgccca cctgcggccc gttaccatga gtgacgataa cttcatgtcc
     4021 ttgggcaatt aaatcaacaa ttgcctgaga agtaattttt accgctttca tttgtccggc
     4081 caggtcatcg cccagcgcat tcccgcccag ggcgagaaca attttcttac tcatttttcc
     4141 tcactcatca ttcttgattg tgtatagatc cctgacagtg gttagagcac gggagggaca
     4201 aacggtttgc gtcgcaggaa acgacctcgc ccggctttgc ctgtaaaggt gccgtcacag
     4261 aaaatcgttt caccacggga taaggttctg acaatcgcgc cctgacaggt aaaaccctcc
     4321 catggcgagt agtcggcgtt gtcgtggaga tggcgatgct gaatttgttg gctctgacgt
     4381 gggtcgataa tcaccacgtc gccatcggaa ccgggcgcta ataatccttt ttgcggccac
     4441 aggccaaaca gcctggcggg cattgcgctg gttaattcaa caaagcgttc cggtgttata
     4501 cgtcccgtca tcacgccact ggaaaacagt aactgcatgc ggttttccac accgggtaag
     4561 ccatttgggc agcgactgaa atcgccttta gaaatttgca ggcgttgagc cattgagaag
     4621 gtgcagtgat cggtcgccac cacgtcaatc gcaccatcgc tgatgccaca ccacagtttg
     4681 tcctgctcgc gtacgttacg cagcggtggg ctaagaatga acttcatgcc gtcttctgta
     4741 tcgtaactgc gttcgtccaa caggagatat tgtgggcagg tttcaaccca gactggctgg
     4801 tgattcgcac gggcaagacg cagataatcc agacctaagc cgttagacag gtgcacgata
     4861 tagagcgggg cgttaccggc aatttgtgcc aggttaatca tgcgggcgat ggcttccgct
     4921 tcgcattcca gagggcgact caaggcatga tagcgcggcg cggttaaccc tgcggcgata
     4981 aattccgccc gcttgctggc gatagccgca tcattttccg ggtgcacggt ggtcagcgcg
     5041 ccggattcat gcagacggcg taatgcctgc aaaacctcgt catcgttgag tttgtattga
     5101 taggttaaat agagtttaaa actgctcagt ccttcctcga ccatcatcgg gatttcgtcg
     5161 aggattgcgt gattaatgtg ctggatcaca ccgtgaaagc tgtaatcgat gaccgcttta
     5221 tgggcggcat aaccacgata aatctccagt tgatggcgta accgacagcc gtttgggcca
     5281 aatcccatat ggtcaataat ggttgttgta ccgccacacg cagctgcgcg ggtaccggta
     5341 aaaaaatcat cacaactgcg cgcgatgccg acatcaatat tgaaatgcgt atggacatcc
     5401 acgccgccag ggaaaacgta acagccagtg gcatcaattt cttcatacgg gagctgcggc
     5461 gaaatattgt tgcccaactg gcgaacaatc ccgctttcaa tcagcaaatc ctgtttggct
     5521 tgtccatctg cgttaacgac agtgccgttt ttgatcaata cgcgcatagc aaactccaaa
     5581 aaccctccgg cgaaccggag gggaataagt gaagagtaat tactctgttg ccagccagct
     5641 taacgggatt gcagcgtaca tcgcggcaca ggtcaccagg tgagatttcc aggttttttc
     5701 gttaggtgca tgcgcttcag gttctttacc cgggccaaag ccgatcaccg gaatgccgtg
     5761 acggcccatg atagatacgc cgttagttga gaaggtccac ttatcaacaa ccggcgcttt
     5821 gccaaacagc ccttcgtagg cattcaccag tgctttaacg gtgaagtgat cttcttccac
     5881 tttccaggtc gggaagtagc attcggttgg gtaaaccagg ccagtccagg acggacggtc
     5941 gtagttgtac atagaaacaa ccgcgttagc tttctgtact gcaggcaggg cgcggatttc
     6001 gtccagcgcg ccttcccagg tttcgcccca ggtcagacgg cggtcaatag agacggcgca
     6061 gctatctgct acagcgcaac ggcttgggga tgtgaagaag atttcagaaa cggtgagggt
     6121 gcctttgccg aggaattcgt cataacccag acgttgggag agttcttgta attcgccaag
     6181 aatcggaccc attttgaaaa tggcgttgtc accgcgttct ggcgcagaac cgtggcagct
     6241 aacaccctga acatcaatac gaatttccat acgaccgcgc tgaccacggt atatctggca
     6301 gtcggttggt tcggtactga ccacaaattc cgggcgaatg ccggattgtt caataatgta
     6361 ctgccagcac agaccgtcgc agtcttcttc ctgcacagta ccggtaacca gcagggtata
     6421 ttcatcttcc agaccgaggt ctttaatgat tttaccggca taaaccatag atgccatgcc
     6481 gccttcctgg tcggaagtac cgcgaccacc gattagctca tcagtttcca tgccttcgta
     6541 cggatcgaag tcccagtttt tgatgttgcc aatgccgacg gtatcgatat gagcgtccat
     6601 tgccaccaga cgcgggccgt ggccgatata accgagaacg ttgcccatcg ggtcgatttc
     6661 aactttatcg aagccgactt tttccatctc ttctttaata cgatgtacta cgcgtttttc
     6721 gtcgcagctt tcactgggaa tagcaaccat gtcgcgcagg aagcgagtca tgtccgcctg
     6781 gtaatctttt gctttttcaa gaatcagttt gaatggaata ttcttagcca ttatttttct
     6841 ccagattttt tactccccgt tttgcaggga gtagttccaa tagggtgatt aaggtgctac
     6901 agcgtgtttg ccttcccaga caacttcgcg gtagtgcttc acgtcggtgt cgccttcagt
     6961 gctgataacc agcaccacgg catctttgtt cagcgccagt ttttccatca ggctttgacg
     7021 ttgcgggtga taatgaaccg ccgcgagaac gcccaaaccg acagcgccgg attcaccgga
     7081 gatgatgcgc gggtcgttgc cgtacggatt acccagcacg cgcataccta atgcggcaac
     7141 gctgtcctgg caggagatga attgggtggc gcagttacgt aggatttccc agcccagcgg
     7201 gttaggttcg ccacaggcca ggcccgccat gatggtggcc atatcaccgc caacgttgac
     7261 gatgtcgcct ttgacgccgg agcgataaat acagtcagct ttgtcaggtt caacaataat
     7321 gctgtgcaga ttttgcgggc tatagacgtc gaccagataa cccagcacac caccggccat
     7381 tgctccgaca ccggcttgca gcagaacgtg cgtcggggtt acgcccattt cacgcatttg
     7441 ctcgacggct tcatctgcca gggttgcgta gccttgcatg atccaggttg ggattttggt
     7501 gtaaccttcc catgccgtgt cctgtaccac ttcccagccg tgctgctgcg cgtgttgcat
     7561 ggtcaggcga acggtatcgt catagttcat atccgtgacg atgcactcgg caccgaggtt
     7621 cagaatggcg tcaacgcgtt cctgagcaga acctttcggc atgtaaatca ccgcattctg
     7681 tccgagttgc tgcgctgccc acgccacacc gcgcccgtgg ttgccgtcgg tggtggtcgc
     7741 gaaagtcatt ttttcgccga tggcattttt caggtgctca aatgacagcg tttcgatatc
     7801 aagatgatat ttttcacaca ataattgagc gatggcgtac gcaccgccga gcattttgaa
     7861 ggcgttcaga ccgaatcgtt ttgattcgtc tttgacaaga attttcttca caccaaaaag
     7921 gtttgcgaga tcgtccagcg cacaaagcgg tgttgggcga taaccagcta ttttctggtg
     7981 gaactggcgc gccagtttgg cctggctttg cgaaaagagc ggtgatgttt cgccgttgaa
     8041 aaatttgtta tcggcgatat caatcttcaa tgagaaaacg gacatatcct atccttttgg
     8101 cctctggaaa tagagggccg accagctgat cggcccgagg ggttatttca cgcgttcttg
     8161 cgcttctttc agcagttgct cgagcagtgc acctggtttg gcgtatttac gggacaggat
     8221 catcgcggcg atgatataag gcttccagct ggcttctttg taggttgcga tacggtattt
     8281 ttcgaatacg ccttcagtca cttcaccttc tttacaggat acgccgctga tatcagctgg
     8341 caggcagtgc atgtacaggg cttcgccatc acgggtcagt tccatcatct cttcagtaca
     8401 atgccagtct ttgtgttgcg cgttctgtgc cagacactgt ttttccagtg cttttaagcc
     8461 ttcgtgatcg ttagcgcgca gcaattcggt acgctgttcc atcactttgt aaggtgccca
     8521 tgacttcgga taaacgatgt ctgcatcttt gaacgcttct tccatgctgg tgacctgacg
     8581 gaagctaccg ccagatgctt tagcattgtt tttcgccact tcaaccacat ccgggatcag
     8641 gtcgtagcct tccggatggg ccagggtgac atccataccg aagcgagtca tcagaccgat
     8701 gatgccttgt ggtacagaga gcggtttgcc atagcttgga gagtaggccc aggtcatggc
     8761 gattttttta cctttcaggt tttccagtga accaaagtgt tcacgtaacc acgcgaggtc
     8821 agccattgac tgagtcgggt ggtcaatatc gcattgcagg ttcactaaag ccggacgctg
     8881 tggcagtaca ccctgcttgt aaccgtcatc aagtgcagcg ccaacttcac gcatataggc
     8941 gttgcctgcg cccagataca tatcgtcgcg aataccaata gcgtcggcgc agaaggagat
     9001 catattggcg gtttcacgca cggtttcgcc gtgagcgatt tgtgatttgc cttcgtcgag
     9061 atcttgttgt gcaaggccga gcaggttaag cgcggaagca taagagaagc gggtacgggt
     9121 ggagttgtcg cggaatacgg aaatacctaa tccactatta aagactttgg ttgagatgtt
     9181 ttcagcacgc agtgctttta atgctgcggc aacgtccagt acttgtttca gttcatctgg
     9241 cgtctgttcc cacgttaaca aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat
     9301 atccttaatc agctcattaa cagttttcat cataaagtcc cggtagtgga atgacaggca
     9361 aagtggttta tttcactgtg acttgccaaa gtgattggtg ttttcacagc tatacctggg
     9421 actaacaata agtgtgccag aattatccac ggggctgcaa aagactaatt tgacggataa
     9481 tgtgtggggt tgatcacaaa ttgatgccag cttccggttg tttgtaaaat acgaaaacgt
     9541 ttggcttttt ttatcgcgtt atttatttgc tgctaagtat cagaaataag ataaagggac
     9601 aggctatctc tcttcagttt gataaactca ccgccgtaat
//
LOCUS       DAZFRN010000173         9577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFRN010000173 DAZFRN010000000
VERSION     DAZFRN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.175"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(89..847)
                     /gene="chbG"
                     /locus_tag="ACHKUB_004256"
     CDS             complement(89..847)
                     /gene="chbG"
                     /locus_tag="ACHKUB_004256"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO6657194.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(860..2212)
                     /gene="celF"
                     /locus_tag="ACHKUB_004257"
                     /gene_synonym="chbF"
     CDS             complement(860..2212)
                     /gene="celF"
                     /locus_tag="ACHKUB_004257"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6657195.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(2317..3159)
                     /gene="chbR"
                     /locus_tag="ACHKUB_004258"
     CDS             complement(2317..3159)
                     /gene="chbR"
                     /locus_tag="ACHKUB_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO6657196.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(3167..3517)
                     /gene="chbA"
                     /locus_tag="ACHKUB_004259"
     CDS             complement(3167..3517)
                     /gene="chbA"
                     /locus_tag="ACHKUB_004259"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO6657197.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(3568..4926)
                     /gene="chbC"
                     /locus_tag="ACHKUB_004260"
     CDS             complement(3568..4926)
                     /gene="chbC"
                     /locus_tag="ACHKUB_004260"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO6657198.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(5011..5331)
                     /gene="chbB"
                     /locus_tag="ACHKUB_004261"
     CDS             complement(5011..5331)
                     /gene="chbB"
                     /locus_tag="ACHKUB_004261"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO6657199.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(5630..5968)
                     /gene="osmE"
                     /locus_tag="ACHKUB_004262"
     CDS             complement(5630..5968)
                     /gene="osmE"
                     /locus_tag="ACHKUB_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO6657200.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            6170..6997
                     /gene="nadE"
                     /locus_tag="ACHKUB_004263"
     CDS             6170..6997
                     /gene="nadE"
                     /locus_tag="ACHKUB_004263"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO6657201.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            7227..8114
                     /gene="cho"
                     /locus_tag="ACHKUB_004264"
     CDS             7227..8114
                     /gene="cho"
                     /locus_tag="ACHKUB_004264"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO6657202.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(8074..8649)
                     /gene="ves"
                     /locus_tag="ACHKUB_004265"
     CDS             complement(8074..8649)
                     /gene="ves"
                     /locus_tag="ACHKUB_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO6657203.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(8852..9337)
                     /gene="spy"
                     /locus_tag="ACHKUB_004266"
     CDS             complement(8852..9337)
                     /gene="spy"
                     /locus_tag="ACHKUB_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO6657204.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         2528 a         2417 c         2153 g         2479 t
ORIGIN      
        1 agcagtctca agcggcgcag ttacgccgcc tttgtaggaa ttaatcgccg gatgcaaggt
       61 tcacgccgca tctggcaaac atcctcactt acacatcccg ataactcccc aaccgataac
      121 cacgctgagc gatagcacct ttcaacgacg ctgatgtcaa cacatccagc tccgttaacc
      181 gtgggaaaca gtaagcactc tgacggatag tattatcgat aaacgccgga tgacacatca
      241 cctccagcga acgatcaccc cgatggcttg cgtcatccag cacttgcagg aacagtgcct
      301 cgctaatctc ttcaccataa aacgcgctac tgaatccctg agaactgcgc agattagccg
      361 gtaaatcacc agcgttagat agcggctgac ggtcgatgcg caacgcaatc ccctcttcag
      421 ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat atgcacatga tgatggctat
      481 caagatgcgt aggtttgcgt ccaaatagct caatgaaacg caaatactga ctggcaagct
      541 cctgagtaat ttcttccagc ggtaaagcat cttcttctgc caactgccag atccattttc
      601 ccagcacacc atcgcgggtt aaccccggca tagctgtcag tggcttgccc atagtaagga
      661 caaagtgcat ccctatggcc agactcggtt catcacgact caactgcacc gcatggtcaa
      721 tagcctgccc attcaccagc gccgtcgttg atgtgacaat cccattgcga caggcctcga
      781 taatgccgta gttctgacct ttgcttaagc caaaatcatc ggcattaaca atcagtaagc
      841 gttccataat cagcctcggt taatgtgctt ttttaagctc tgcgatgcag tcggcaaaat
      901 ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc cagcagctca gcatcgcgat
      961 cggaatgcac caacggacta aggtttagcg ccagtaacac atcgttaaat tctccgctaa
     1021 gtgcggcgtt gctggcagca atctcgaagc ctttaatggt gtgaatcagc cccatcactt
     1081 tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc agcttacagg
     1141 tcatttctac cgcccagtct gccggaatat tatcaatatg cccatgatgc gggatattaa
     1201 cgtaatgttc agcttgcttg tcgttgtaga tagcgttgat cacttcgcac gctgcatcag
     1261 agtaataagc cccaccgcgc tgttccagtt ctttcggctt aactttcaac tccggatttt
     1321 tatacagctc aaaaagttgt ttctcgactt tctgtactac ctgtgctcgt gcgccgcctt
     1381 tgtagtattc gcccatttca atagccagca tctctttctg cttgaagtaa tacagcagat
     1441 aagaacatgg cagcagattt aacgaacgaa ttaagccctc actaaatggc agatcgaaaa
     1501 tattttttac agaggacgct tttaactgcc ctgacgccac accatcaagc aattcggcaa
     1561 agcgcgactt gccatttacc agcacatcct taatgaacac catatggttg aggccgaaca
     1621 gatcgataga taaatcatcg ctgtctttca gcatcagaac atcgcgaata aacatcttca
     1681 tgccgatcgg aatattacac acgccgataa agcgtttaaa tccggtatga cgataaacgg
     1741 cttcagtgac cattcccgcc gggttagtga agttaatcac ccatgcattc ggacaaagtt
     1801 cttcgacatc ttttacgatg tcaaaaatca ccggaatggt acgcagacct ttaaacagac
     1861 cgcccgcgcc gttggtttcc tgaccaagat aaccatgact taatggaata cgttcatcca
     1921 gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt aacgaaatca gcatctttca
     1981 atgcttcgcg gcgatccagc gttttataaa gcttcatcgg gacgccagcg ttatcaatca
     2041 tccgttggca gagttcaaaa atgatatcca gtttctcttt accatcttcg acatccacca
     2101 gccataattc gctgaccggc aattcgtgat aacgcttaat aaatccttcc agtaactccg
     2161 gggtatagct gctcccgcca ccaatagtga cgacttttaa tttctggctc ataatttctc
     2221 ccttcagtac agaatactga tatctggcat atctgccccc ccggacataa ataatccagc
     2281 aacaggacag atatgtgaat tgtcaggtat aacgacttac tgattaaatt cagtcaattt
     2341 cttacgatag ctcttaggcg taaaggacgt taatttctta aacgttttaa taaacaaact
     2401 cgggctacta taaccggcct caaacgcaat atccgtcacg gaatagttgg tcatttccag
     2461 ttgttttttg gcaaaattaa tacggatctc attaataatc tgcattggtg ttttgccata
     2521 atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg gctgacaacg tcaccatatt
     2581 ctccagcgcc gattcactaa actgctcttt atcatgcatc ttttctaccg tacttttcag
     2641 ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac ggttaatgac
     2701 ataaaaagta accatctcaa caaactcttc cagccctgtt tcgcggaaat tcaatgaaga
     2761 aatcactgtt tccacatagg tgagaaaagc gttattggtc cggtatacct gcgaagcgac
     2821 aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa aagcgtttac tgatcccaac
     2881 gttcaatata cgcgtggcac caaactcata aaaactttga tggtgcgaac ctaacggaat
     2941 aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct ggaaataacg
     3001 cccggttaat accagagtaa attcataata gtcgtgctga tgcaacccgc tgatactctc
     3061 agttttgtta tagataaaca catggaagtt tttgccatta aacaactgct gttctcgggc
     3121 agtggcaatt tccggcgcgt taatcactgg ctgcatcatc gactccttat gccttcagtt
     3181 tttcatgaag ctcaattaat tcagtaatca gttcacgcgc aagcatggac gtcattaaat
     3241 gatcctgagc gtggaccagc accagactca ctttcatctt accttcgccc gcatcgcctt
     3301 caatcagttt cgtctgtacc agatgcgctt cattcaatgc catccgtgac tgatccatca
     3361 tggcttttgc tgcagcaaaa tcgccctgct tcgcctgttt cagtgccgca tacgccaggc
     3421 tgcgcgcttg tccggagttg atgataagcc ccatcaccac ttcttccagc tcttcagctt
     3481 ccgtttgcgt atcgggaatg ttatcgagat ccatcataca tcgttcctct ctattcttac
     3541 cggcacgatt acccgtaccg gcatcgatta aaatttcagg gcgttagcga tatcttcttc
     3601 gctctcttct ttatcaatcg cgttttgtgc tttgttagcc accacaacaa agggcagata
     3661 aattaacgtt gcgataccaa ggttgaagag tgcgaccagc aatgcggcga cgctaccgtt
     3721 ggtgttaaag aaggctccca gaccggttgg catggtccac ggtgcaatat tggtcaccgg
     3781 aggaatgatg cccatgtagt acgctgcgag ggtgattgcc gccagaatcg gttgtaccag
     3841 tacaaacggg ataaacatca ccgggttcat gataattggc agaccaaaca gaatcggttc
     3901 gttaatctgg aagatgccgg acggcagcgc cagttttgcc acctgacgat aatcagcacg
     3961 gcgagaggcg ataaagatag ccaggatcag gcctaaagtc gcaccactgc ccccaaggaa
     4021 aataaaggag tccagcatcg gcttggccca gatatggaag gtcttaccgg ctgccagcgc
     4081 cgcttcgacg gaaccatatt gctgataggt cgcgatattt tccagcgccc acggcgtcat
     4141 aatgccgttg tccagtgcgg tcagcgccag cgcgccatga ataccgaaga accagagcag
     4201 tggaacaaag atcacatagg cccagcccac cacgctaccc aacgatgcca gtggggttga
     4261 gatggtatcc ataatgatct gatggaagtt ggtgccccag gtattcaacg cccaggcaat
     4321 aatccccatc acggaaagaa taataaagcc gggaattaat gccgagaagg aacgcgatac
     4381 tgaagcaggt acgctatcgg gcaatttaat gacccaattg cggcggacaa taaaggtaaa
     4441 catttctgcc accaccaggc caataataat cccggagatg atattcgctc cacctaacca
     4501 gtttgcgcca accgcatagg cctcaccgac actatatggg gtgacggtca taaatgctgc
     4561 aacggataac aacccagccg ccagcgcatc gactttacgc tcttctgcca gcgccatgcc
     4621 aataaagaac ggtgccatta aagacattat ccctaatgtt ccgttatata cgttgccgcc
     4681 aatacctttc aggccattaa gtgtttcaat ggttgaggca tctaggcgaa tacctaagga
     4741 ataaaaaaat gacccctccc caaagcttag aaaaacgttg ttaattaata caaacatggc
     4801 ccccgcaagg gttaacggca ttaagcgaat aaagccattt ttgattgcat taacgtgtgg
     4861 ctgctttcct attttaactg caaaagggag gagtaccttt tcaagcgatg caataacatt
     4921 actcatagaa aaataccctt aaaaaccgca atttaaatat tgcggtattg atttatgaaa
     4981 taactctttg acgggaaaat ttaaaataaa ttaatttgct gcggcttttt taatcgctgc
     5041 aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg agtcaattac
     5101 ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag caatctgcgg
     5161 ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt ccggaaatgc
     5221 ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg ataccagtaa
     5281 agaggtagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca taaaactgcc
     5341 ctcatcgacg attatctgtc agccagacac tccgcaagcc ttaacctgct tccatgctct
     5401 gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata cggtatagac
     5461 ctgagcaata gtatttcttt gaccttataa attgtttctc cttgacctgt ggttatgacc
     5521 ctcttcacat ttcgggcaaa taattcgcga agataattaa gaactcaggc caaaaaaaac
     5581 cggcgcaatg gccggtttcc gttgttactc aagctttcag acgaattgat tacttcgcag
     5641 cctgtggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg acatgtccgg
     5701 tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc aggatgtagg
     5761 tctggcaagt accgcgagca tggatcatgc tcacttcaga cgaaggttta cccgcaattt
     5821 gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc tgtacaaact
     5881 ggtctttggt acgatcataa gccgtacaac ccgccagcat ggttaatacc gccgctgcac
     5941 tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag cgtgttagat
     6001 aagcctggaa tacattgggc gctttttcaa gcccgtgaac gaaacggctc cgctttcaga
     6061 ggattcctgt atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc
     6121 aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa tgacattgca
     6181 acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag aggaaattcg
     6241 tcgtagtgtc gattttctga aaagctacct gcaaacttat ccgttcatta aatcactggt
     6301 gctcgggatc agcggcggtc aggactccac gcttgccgga aagctgtgcc agatggcgat
     6361 taatgagctg cgccaggaaa ctggcaacga atcactgcaa tttattgccg tacgcctgcc
     6421 ctatggtgtt caggccgacg aacaagattg ccaggatgcc attgccttta ttcaaccgga
     6481 tcgcgtatta accgttaata tcaagggcgc ggtattggct agcgagcagg cattgcggga
     6541 agcaggcatt gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa
     6601 agcacaatat agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg atcatgcagc
     6661 agaagccatt accggattct tcactaaata tggtgacggc ggtacagaca ttaatccgct
     6721 gtatcgtctc aacaaacgtc agggtaaaca gttactggcg gcattaggtt gcccggaaca
     6781 cctttataag aaagcgccaa cggccgatct ggaagatgat cgcccttctc tgccagatga
     6841 agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga aaaacgtacc
     6901 tgaacaggtc gccagaacaa tagagaactg gtatctgaaa accgaacata aacgccgtcc
     6961 gccaattacc gttttcgatg atttctggaa aaagtaataa tttgcaccta ttggattgtg
     7021 cgaaaaagag taatttgttc acgccggatg cggcgtgaac gccttattcg acctacaaaa
     7081 ctatgcaaat tcaatacatt gcaggagtcg aataggcctg acaggcgtag cacgtcagac
     7141 ggtgtaacct ttgtcatcaa cccgcctctt ttttaatcgc ctcccgcctg ttacactgga
     7201 tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg cggctcgaat
     7261 ttgaagctgc ggcaatttat gaatatcccg aacatttacg ttcattcctt aatgacttac
     7321 ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg ctctatatcg
     7381 gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg gatgaagccg
     7441 ccatgctacg gcaatcccgg cgaattagct ggatctgcac cgcaggcgaa atcggcgctc
     7501 tgctccttga agcgcgatta atcaaagaac aacagccgct gtttaataaa cggttgcgcc
     7561 gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg gtgtatgcca
     7621 aagaggtgga tttttcacga gcccccaacc tgtttggcct gtttgccaat aggcgcgcag
     7681 ctttgcaagc attgcagagc atcgctgatg aacaaaaact ttgttatggc ctgttgggac
     7741 tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt tgcgccggag
     7801 catgctgcgg taaagagagc catgaggaac atgcgctacg cttgcgccag tctctggagc
     7861 gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc actgaaagaa cagcacccgg
     7921 aaatgactca atatcatatt attcaaaact ggctgtggct gggggcggtt aattcgctgg
     7981 aagaagcgac aacgttaatt cggacacccg ccgggtttga tcacgacggt tataaaattc
     8041 tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg gcgaatgacc
     8101 agcgagccag ttgatttcac tgaacaacag tttaccttgc ggttgtaaaa gccgcagggt
     8161 gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg agcaatttat cgcccagttg
     8221 ccacgccccg ttaatcacaa acaccacccc gccgcgtgag ccaaaggtag taaacgtacg
     8281 ttcggcaatt ctgactttcg ccttacagac atccaggcgt gtcatgatgt tgaaatccat
     8341 cgacatctgc cctgccgtca gtttcgcttt taccacctgg tccgccgcaa aggcaaaagg
     8401 ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt tcaaggaaca tctcgccgcc
     8461 ttccagcaac gtcactatcc tttccatgcc gggaaacaaa gaaaactccc cattcgccgc
     8521 gatggatgca atgctggcac gccagtaaaa atcacgtttt gccggtggga aagtgcaaat
     8581 ttcgcgcgtt tcaccggcag cgtttcgcca cagattcacc gacattttac gcatatcaaa
     8641 gtattccata ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt aaccgtggat
     8701 ggttatcggc aactcgggtg atttacgtta gtggtgatca ggcttttttt acaactgttt
     8761 gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag
     8821 accggcggtc ttaagttttt tggctgaaag attattcagc agttgcaggc attttacctt
     8881 ttgccgctgg acgttctgtc agacgcttct caaaattagc attaaattgc tttttctgtt
     8941 ccggcgtcag gatgttgtaa attttgttct gggtttccat gtgcgccagc atgttagctt
     9001 tgcgctgttc ttccattttt gcgatctgcg cttcagcttt tgctttatcg aaggtatcgc
     9061 tggcaatgat gtcatgcatt gcgcggcgtt cttccagcgg cggacgtttc atctggtcac
     9121 gctggccttt catgatttcg cggatctgct gtttctgcgc gtcggtcagg ttcaggtctt
     9181 tgaacatcat gtcctgatgc ggaccgaact tgcctttgtg gtgcatcatc ggcttcgcgt
     9241 cagccggtgc tgcggtagtg gtgtctgcgg catgggccag gttagccgcg ccaagagcca
     9301 gggtagaggc aacaaacagt gcagttaatt tacgcatatt ctatatcctt cctttcagtt
     9361 atttattacg gctttcttaa gtagcgtgcc gtgttgacga gattaacttt actgacttta
     9421 gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa
     9481 ttatggagaa aaaaagaaaa tttgatggag agtgatgaga gaatattaca acacgatgat
     9541 tttgcagaga ttatgaagaa ctataccgga tgactgg
//
LOCUS       DAZFRN010000174         9417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFRN010000174 DAZFRN010000000
VERSION     DAZFRN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.176"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            219..359
                     /gene="ymiA"
                     /locus_tag="ACHKUB_004267"
     CDS             219..359
                     /gene="ymiA"
                     /locus_tag="ACHKUB_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO6657205.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            362..529
                     /gene="yciX"
                     /locus_tag="ACHKUB_004268"
     CDS             362..529
                     /gene="yciX"
                     /locus_tag="ACHKUB_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO6657206.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            643..738
                     /gene="ymiC"
                     /locus_tag="ACHKUB_004269"
     CDS             643..738
                     /gene="ymiC"
                     /locus_tag="ACHKUB_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO6657207.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            902..3577
                     /gene="acnA"
                     /locus_tag="ACHKUB_004270"
     CDS             902..3577
                     /gene="acnA"
                     /locus_tag="ACHKUB_004270"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO6657208.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3641..4231)
                     /gene="ribA"
                     /locus_tag="ACHKUB_004271"
     CDS             complement(3641..4231)
                     /gene="ribA"
                     /locus_tag="ACHKUB_004271"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO6657209.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4401..5165
                     /gene="pgpB"
                     /locus_tag="ACHKUB_004272"
     CDS             4401..5165
                     /gene="pgpB"
                     /locus_tag="ACHKUB_004272"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO6657210.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5314..5622
                     /gene="lapA"
                     /locus_tag="ACHKUB_004273"
     CDS             5314..5622
                     /gene="lapA"
                     /locus_tag="ACHKUB_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO6657211.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5629..6798
                     /gene="lapB"
                     /locus_tag="ACHKUB_004274"
     CDS             5629..6798
                     /gene="lapB"
                     /locus_tag="ACHKUB_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO6657212.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6991..7728
                     /gene="pyrF"
                     /locus_tag="ACHKUB_004275"
     CDS             6991..7728
                     /gene="pyrF"
                     /locus_tag="ACHKUB_004275"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO6657213.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7728..8054
                     /gene="yciH"
                     /locus_tag="ACHKUB_004276"
     CDS             7728..8054
                     /gene="yciH"
                     /locus_tag="ACHKUB_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO6657214.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8180..8398)
                     /gene="osmB"
                     /locus_tag="ACHKUB_004277"
     CDS             complement(8180..8398)
                     /gene="osmB"
                     /locus_tag="ACHKUB_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO6657215.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8667..9416)
                     /gene="yciT"
                     /locus_tag="ACHKUB_004278"
     CDS             complement(8667..9416)
                     /gene="yciT"
                     /locus_tag="ACHKUB_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO6657216.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         2347 a         2203 c         2452 g         2415 t
ORIGIN      
        1 catatttata gggtaaatta cctgtaaagt agtgtctaat tacccgcctc gcaaactcca
       61 gttttcaaat atctatttca ggtcaaaaga ttgaattatt cactgagacg attcgtaaat
      121 tcgctggatt tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc
      181 aatcgttcaa cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg
      241 gaaatcagga accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc
      301 ttggttctgc acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac
      361 tatggtcggt caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac
      421 agaaacgaaa cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca
      481 gcaagccttt atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt
      541 cttattaccc atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg
      601 agttatctca acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg
      661 aaatattggt cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc
      721 tggatcatta ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac
      781 aaggtttctc ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt
      841 atctttaata ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc
      901 tatgtcgtca accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta
      961 ccactactac agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa
     1021 gtcactcaaa gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga
     1081 agaggatatc cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc
     1141 ctaccgcccg gcaagggtgc tgatgcagga ctttaccggc gtacctgccg ttgttgatct
     1201 ggcggcaatg cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct
     1261 ctcaccggtc gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga
     1321 ggcatttgaa gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct
     1381 gaaatgggga aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg
     1441 ccatcaggtt aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga
     1501 atggattgct tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg
     1561 ccttggcgtg ctggggtggg gcgtgggtgg gatcgaagca gaagccgcaa tgttaggcca
     1621 gccggtttcc atgcttatcc cggacgtagt gggctttaaa cttaccggta aattacgtga
     1681 aggtattacc gccacagacc tggttctcac tgttacccaa atgctgcgca aacatggcgt
     1741 ggtggggaaa ttcgtcgaat tttatggtga tggtctggat tcactaccgc tggcggatcg
     1801 cgccaccatt gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga
     1861 tgctgtaacc ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg ttgagttggt
     1921 cgaaaaatat gccaaagcgc agggcatgtg gcgtaatccg ggcgatgaac cgatttttac
     1981 cagtacgtta gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgccc
     2041 acaggatcgc gttgcactgc ccgatgtacc aaaagcattt gccgccagta acgaactgga
     2101 agtgaatgcc acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta
     2161 tcagttacct gatggcgctg tggtcattgc tgcgataacc tcgtgcacca acacctctaa
     2221 cccaagtgtg ctgatggctg caggcttgct ggcgaaaaaa gccgtaactc ttggcctcaa
     2281 gcggcaaccg tgggtcaaag cgtcgctggc accaggctcg aaagttgttt ctgattatct
     2341 ggcaaaagcg aaattgacac cgtatctcga cgaactgggg tttaaccttg tgggatacgg
     2401 ttgtaccacc tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa
     2461 aaaaggcgat ttaaccgtcg gtgcagtgct gtccggcaac cgtaactttg aaggccgtat
     2521 ccatccgctg gttaaaacca actggctggc ctcgccgccg ctggtggttg cctatgcgct
     2581 ggcgggaaat atgaatatca acctggcttc tgagcctatc ggccatgatc gtaaaggcga
     2641 tccggtatat ctgaaagata tctggccatc ggcacaagaa attgcccgtg cggtagaaca
     2701 agtctccaca gaaatgttcc gcaaagagta cgcagaagtt tttgaaggca cagcagagtg
     2761 gaaggaaatt aacgtcacac gatccgatac ctacggttgg caggaggact caacctatat
     2821 tcgcttatcg cctttctttg atgaaatgca ggcaacacca gcaccagtgg aagatattca
     2881 cggtgcgcgg atcctcgcaa tgctggggga ttcagtcacc actgaccata tctctccggc
     2941 gggcagtatt aagcccgaca gcccagcggg tcgatatcta caaggtcggg gtgttgagcg
     3001 aaaagacttt aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac
     3061 cttcgccaat attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg
     3121 gcatttacct gacagcgacg tagtctctat ttatgatgct gcgatgcgct ataagcagga
     3181 acaaacgccg ctggcggtga ttgccgggaa agagtatgga tcaggctcaa gccgtgactg
     3241 ggcggcaaaa ggtcctcgtc tgcttggaat tcgtgtggtg attgccgaat cgtttgaacg
     3301 aattcaccgt tcgaatttaa ttggcatggg catcctgccg ctggaatttc cgcaaggcgt
     3361 aacgcgtaaa acgttaggac taaccgggga agagaagatt gatattggcg atctgcaaaa
     3421 tctacaaccc ggtgcgacgg ttccggtgac gcttacgcgc gcggatggta gccaggaggt
     3481 cgtaccctgc cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg
     3541 cattttgcat tatgtcattc gtaatatgtt gaagtaacaa gtatttgctt gccggttatt
     3601 ttgcttccgg caagcaaact aattacacaa tgcaagaggg ttatttgttc agcaaatggc
     3661 ccattttctc ggctttggta tcgagataat gttcgttatt ggggttacga cctacaatca
     3721 atggtacgcg ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt
     3781 tattggttaa caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag
     3841 tgaagtcgcg ctcatcagcg gcgaagccta actggtggtt agcctctacg gtatcgtaac
     3901 cttgatcctg cagtgcgtaa gcgcggattt tattcagcag accaatgtta cgaccttcct
     3961 gacggtgata cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat gccgcttcga
     4021 gctggaagcc acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat
     4081 ggacgcgcgc aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat
     4141 cgtgtccggt tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca
     4201 gtttggcttc tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta
     4261 ttggcttatt ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac
     4321 ggaatggcac aaatctgtca atttttcctg gaactggcgt tttcagttat gattgtggga
     4381 cttatcaaaa aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac
     4441 tattgcttgt catgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac
     4501 aaagttggct actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg
     4561 tcattacaca tttgatttta ttcggctggt ttctctggtg tctgcgtttt cgcattaagg
     4621 ctgcctttgt attatttgcc attctggcgg ccgcaatcct tgtgggacaa ggcgttaaat
     4681 cctggatcaa agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaacac
     4741 atcatattcc ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga
     4801 aagaacagtt ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcagaaag
     4861 agacggggtt tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gcactgctgg
     4921 ccgttggttt gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg ctggtctggg
     4981 caacgggagt catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtag
     5041 tagctacgtt gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt
     5101 gtgggccatt aacaccacct gcggaagaaa atcgcgaaat agcgcaacga gaacaagaaa
     5161 gttaacgctg gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc
     5221 catttcgcag acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa
     5281 tttattcgct ctaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg
     5341 gtgttagcga tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca acaggtgacg
     5401 tttaattatc tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt
     5461 gctgcggggt ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg agttcgtgtt
     5521 tccctggcgc gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact
     5581 gacgtggctg tagtgccgca ctcgtcagcg gcgaaggaat aactttctat gctggagttg
     5641 ttgtttctgc ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg
     5701 caacaaaaca agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac
     5761 ttcctgctta gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag
     5821 gatacaggca ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa
     5881 gttgatcgcg ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct gacctatgaa
     5941 cagcgtctgt tggcgattca acaactaggg cgtgattaca tggccgccgg gttatacgac
     6001 cgcgcggaag acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgctctg
     6061 caacagttgc tacaaatcta ccaggctacc agtgagtggc agaaagcaat tgatgttgcc
     6121 gaacgcctgg tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt
     6181 gagttagccc tgcagcatat ggccagcgac gatctcgatc gtgccatgac cttgctaaaa
     6241 aaaggggcgg cggcagataa aaacagcgcc cgcgtatcca tcatgatggg acgcgtgttt
     6301 atggcgaaag gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat atcccaggac
     6361 agagaactgg tcagcgaaac gctggaaatg ttgcaaacct gctatcagca gttgggtaaa
     6421 actgccgaat gggcagaatt cctgcaacgc gcggtggaag agaacaccgg tgccgatgct
     6481 gaattgatgc ttgctgatat catcgaagcg cgcgacggta gtgaggccgc acaggtctat
     6541 attacgcgcc agcttcagcg tcatccgacc atgcgtgtgt tccataagtt aatggattac
     6601 cacttaaatg aagcggaaga agggcgtgcc aaagagagcc tgatggtgct gcgtgacatg
     6661 gttggcgaaa aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca
     6721 tacaccctct actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc
     6781 ggtcttgatg gcctgtaatt tttaaaaaaa tcctacttta gttacaacat actagttatt
     6841 gatgttccat tgtgctccgg caacgacggc gcagaaaaag cctgccaggg gaaaaatcgc
     6901 aactgttaat tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact
     6961 tccggtgccc atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc
     7021 gctgttacga attctcctgt ggttgttgcc cttgattatc ataatcgtga tgacgcgctg
     7081 tcctttgtcg acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt
     7141 acattgtttg ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt
     7201 gacctgaaat tccacgatat tcctaacact gcagcgcacg ctgtcgctgc tgcagctgac
     7261 ttaggcgtgt ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg
     7321 cgtgaggcac tggttccgtt tggcaaagat gcaccgcttt tgattgctgt gacagtgttg
     7381 accagcatgg aagccagcga cctggtcgat cttggcatga cactgtcacc tgcagattat
     7441 gcagaacgtc tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct
     7501 caggaagctg tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacgccgggc
     7561 attcgtccgc aggggagtga agctggtgac cagcgccgca ttatgacgcc agaacaggcg
     7621 ttgtcggctg gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca
     7681 gcgcagacgc tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca
     7741 acagccgtct ggtctactca acggaaaccg gacgtattga tgagcccaaa gcggcccctg
     7801 tacgccctaa aggcgacggt gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta
     7861 agggcgtttg cctgattacc ggtgtcgatc tcgatgatgc cgaactgaca aaacttgcag
     7921 cggaactgaa gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt attgaaatcc
     7981 agggcgataa gcgtgattta ttaaaatcac tacttgaagc gaaagggatg aaggtaaaac
     8041 tcgcaggcgg ttaacataaa aagccacgga tatatccgtg gctttcgaat attttactgt
     8101 gcgtattatt cagttatttc gtactgaaca gaatcaagcg tattataatt gcgagtcgat
     8161 aatcgacatt tatttacgat tatttaccga cctggtgacc aataacacca ccgacagctg
     8221 caccacctaa tgtacccaac gtactgccat cggtcagtac tgcaccgcct aatgcccctg
     8281 cacccgcgcc gattgcggtg ttgcggtccc gtttagacca gttagaacag gcactcagag
     8341 acattgccaa agtaattgcc agaacagccg cggtcatttt tttgctcgtt acaaacataa
     8401 tactctctcc tgaatttatg attcacggaa gtaagctctc tataactata atagctaaga
     8461 ataagcctgg tgaaattatt ccgtgaaatc tgctcgcgcg caggattata tcacgcaggt
     8521 gataatgact tcctgttata tcgctgataa taattttata tcttaagcgt gttaataaca
     8581 ggtaaatagt cttaattatc aaccaggaat catcttagag cggatgattt gccaaactgc
     8641 aaatcatccg tagagaaggg aaatggttaa atgtcaacga cgtgaacggt gagtttactg
     8701 tgctcaagat gcatcagatc gctggcgcgt attttcgaat cggtaatcac gcgattgaat
     8761 cgctcaacgg gaccgatgga gtatgaatgt acagcaccaa atttcgagct gtcagtcagg
     8821 actatcgctt cgcactcttt ttccagcacg gcattgacca catcggtacg catcatatcg
     8881 cgaccggtga atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa
     8941 tgtacctgct ggatgcactg gcgtgtcaaa gggccaacca tactttcgct ttttttctgg
     9001 tacacgccac cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcaatgtag
     9061 ctgctgactg tgatgatagt gacatttttc ttctgttcgc ccagagtccg ggcgagcagg
     9121 gcattgctgc taccattttc gataaagatg gtttcgcccg gttgaaccag agacgcggca
     9181 aactcggcga gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca
     9241 ctatcaagcg aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg
     9301 ttgagatcct ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg
     9361 ctaacctgac cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatc
//
LOCUS       DAZFRN010000175         9035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFRN010000175 DAZFRN010000000
VERSION     DAZFRN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.177"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..876
                     /locus_tag="ACHKUB_004279"
     CDS             <1..876
                     /locus_tag="ACHKUB_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO6657217.1"
                     /translation="ECFINWMRDCHSSNDKDVIAIDGKTLRHSYDKSRRRGAIHVISA
                     FSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGG
                     DYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDEL
                     IDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHV
                     ENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAM
                     DRNYLASVLAGSGLS"
     gene            1145..3382
                     /gene="nrfB"
                     /locus_tag="ACHKUB_004280"
     CDS             1145..3382
                     /gene="nrfB"
                     /locus_tag="ACHKUB_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO6657218.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3369..6341
                     /gene="nfrA"
                     /locus_tag="ACHKUB_004281"
     CDS             3369..6341
                     /gene="nfrA"
                     /locus_tag="ACHKUB_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO6657219.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQKRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6342..7232
                     /locus_tag="ACHKUB_004282"
     CDS             6342..7232
                     /locus_tag="ACHKUB_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO6657220.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAAHQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7415..8176
                     /gene="envY"
                     /locus_tag="ACHKUB_004283"
     CDS             7415..8176
                     /gene="envY"
                     /locus_tag="ACHKUB_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO6657221.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(8222..8298)
                     /locus_tag="ACHKUB_004284"
     tRNA            complement(8222..8298)
                     /locus_tag="ACHKUB_004284"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8262..8264),aa:Arg,seq:tct)
     gene            8541..>9035
                     /gene="fimZ"
                     /locus_tag="ACHKUB_004285"
     CDS             8541..>9035
                     /gene="fimZ"
                     /locus_tag="ACHKUB_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO6657222.1"
                     /translation="MKPTSVIIMDTHPITRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGL"
BASE COUNT         2324 a         2270 c         2291 g         2150 t
ORIGIN      
        1 gagtgcttta ttaactggat gcgtgactgc cattcatcaa atgataaaga cgtcattgca
       61 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      121 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      181 aaatctaatg agattacagc tatcccagaa cttcttaaca tgctggatat taaaggaaaa
      241 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      301 ggaggtgatt atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      361 gaaaaatttc cgctgaaaga attaaataat ccagagcatg acagttacgc aattagtgaa
      421 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      481 attgatttca cgtttgaatg gaaagggctg aagaaattat gcgtggcagt ctcctttcgg
      541 tccataatag cagaacaaaa gaaggagcca gaaatgacgg tcagatatta tatcagttct
      601 gctgatttaa ccgcagagaa gttcgccaca gcaatccgaa accactggca cgtggagaat
      661 aagctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga
      721 aatgcagcag aattattttc agggatacgg cacatcgcta ttaatatttt gacgaatgat
      781 aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga cagaaactat
      841 ctcgcgtcag tccttgcggg gagcgggctt tcgtaatctt gccctgcctg ctcacctcct
      901 ccaggcactt ccactacatt atgacctgct cggtgccacc gctccttttc actatgacgt
      961 taacctcccc tccttgacac catgcttttg tctacattct acgcaaacgg caacttttat
     1021 aaccagtgta aaaataacgt gccgcaatag tcatacctat taatggtaaa aagctgtcac
     1081 aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa tgtaaggttt
     1141 ttgtgtggac tggcttcttg atgtttttgc aacctggctc tacggcttaa aagtaatcgc
     1201 gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta ttgatgtcgt
     1261 ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc cgcgaatgag
     1321 ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc cggcgtggaa
     1381 tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg actacgaaaa
     1441 ctatcatatc tttgttggca cctaccccaa cgaccccgat actcaacgtg atgttgacga
     1501 agtgtgcgct cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg gtcccaccag
     1561 caaagccgac tgtctgaaca acgtgctgga cgccatcacc cagtttgaac gtagcgccaa
     1621 tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac cgatggaatt
     1681 gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccgg tgtatccgtt
     1741 cgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt cagagctgca
     1801 tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtgcccagcg caggcgtcgg
     1861 cacctgtttc agccgccgcg ccgtgaccgc tctgttagct gacggtgacg gtattgcttt
     1921 cgacgtgcag agtctgactg aagattacga cattggtttc cgcctgaaag agaaaggtat
     1981 gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg agcagcgtaa
     2041 atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt tccccgatac
     2101 cttttcgact gcagttcgac aaaaatctcg ctggatcatc ggcattgttt tccagggctt
     2161 taaaacccac aaatggacct ccagcctgac gctgaactac tttctctggc gcgaccgcaa
     2221 aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgctcc agcttttgct
     2281 gttgctggcg tatgaaagtt tgtggcccaa tgcctggcat ttcctttcta tttttagcgg
     2341 cagcgcatgg ttaatgaccc tgctgtggct aaactttggc ttgatggtta accgcatcgt
     2401 gcagcgggtg attttcgtca ctggctacta cggtctgacg caggggctgc tatctgtcct
     2461 gcgtcttttc tggggcaacc tgattaactt catggccaac tggcgcgcgc taaaacaggt
     2521 acttcaacac ggcgatccac gtcgcgtcgc gtgggataaa acaacgcatg acttccccag
     2581 cgtgactggc gatacccgct cgttgcgccc gttaggccaa attctgctgg aaaatcaggt
     2641 catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc tacgcctggg
     2701 cggttcaatg ctgatgcagg ggctgattag cgccgagcag ctggcacagg cgctggcaga
     2761 gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccgtcct cgctgattgc
     2821 cgaaatgcca gcctccgtag cgctgcatta tgcagtactg ccgctgcgtc tggaaaatga
     2881 tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg ccctgacgcg
     2941 taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg tcacggggtt
     3001 acgtcactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt acaatgcggt
     3061 tcagcatcag tggctcacgg aacagcagac cggtgaaatc tggcggcaat atgtgccgca
     3121 tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt cagcaattaa
     3181 cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt tggtcaccga
     3241 aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg aattacaagt
     3301 ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg cgcaactgga
     3361 gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt ttactgctga
     3421 cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca gaagagctgg
     3481 ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg ctgaaggcac
     3541 agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac cagcaggtgc
     3601 cggataatat tccgctgact ttataccttg cggaagccta tcgccatttt ggtcatgatg
     3661 accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat gcccgacttg
     3721 agcgcagtct ggcggctatt ccggttgaag tgaaaagcgt tacgactgtt gaagaactgc
     3781 ttgcccagca aaaagcgtgc gacgctgcgc cgaccctgcg ttgtcgcagt gaagtcgggc
     3841 agaatgccct gcggctggca caattacctg tcgccagagc gcaactgaac gatgcgacgt
     3901 ttgctgcatc gccagaagga aaaacgctgc gaaccgatct gctgcaacgg gcaatctacc
     3961 tgaaacaatg gtcccaggca gatacgctat acaatgaagc acgccagcag aacacattaa
     4021 gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagctg gacgatcgga
     4081 tcctggcact gcaatcacag gggatcttca ccgatcctca gtcatatatt acttacgcga
     4141 ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcaat gaaaataagc
     4201 cgctgtttac cacggacgca caagagaaaa gttggctcta tctgttatct aaatacagcg
     4261 ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac cgccagtatg
     4321 ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca gcgcaaaaac
     4381 tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc agcgtggcga
     4441 cccgtaacaa ggctgaatct ctgcgtctgg cacgattgct gtatcagcaa gaaccggcaa
     4501 atcttacccg cctggatcaa ctgacctggc aactgatgca gaacgagcag tcacgcgaag
     4561 ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtgtc agccagactt
     4621 taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctggcaacg ccggcgaagg
     4681 tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa agtcagttgc
     4741 cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg tcgccttcct
     4801 acgatgctgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta cccggtgtgg
     4861 cgctgtatgc atggcttcag gccgaacaac gacaaccgaa cgcctggcaa catcgtgcgg
     4921 tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc tggcagaaaa
     4981 tcagtcttca cgacatgagc aatgaggatc tgcttgctgc tgccaatacc gcccaggcgg
     5041 caggaaatgg tgcagctcgc gatcgctggc tacaacaggc agaacaacgt ggactgggaa
     5101 acaatgccct ctactggtgg ctgcatgcgc aacgttacat tcctggtcag ccggaactcg
     5161 cactgagcga tctcacgcgc tcaatcaata ttgcgccttc tgccaacgct tacgttgcgc
     5221 gggcgacaat ttatcgccaa cgtcataatg tcccggcggc ggtgagtgat ttgcgcgccg
     5281 cgctggaact ggaaccgaat aatagcaaca tccaggcagc gcttggttac gccttgtggg
     5341 atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa gggcttccgg
     5401 acgatccggc actgatccga caactggcct atgtgaacca gcgtctggat gacatgcctg
     5461 cgacgcagca ttacgcccgg ctggtgattg atgacattga taatcaggcg ctgataaccc
     5521 cactgacccc agagcaaaat cagaaacgct tcaatttccg ccgtttgcat gaggaggtcg
     5581 gtcgccgctg gacgttcagt ttcgattctt ctatcggctt gcgttccggg gcaatgagca
     5641 ccgctaacaa taatgtcggc ggcgcagcgc cagggaaaag ctatcgtagc tacgggcaac
     5701 tggaagccga gtaccgcatc ggacgcaata tgctgctgga aggcgacctg ctctcagttt
     5761 atagccgcgt ctttgccgat accggagaaa acggggtgat gatgccggtg aaaaatccga
     5821 tgtccggcac cggtctgcgc tggaagccgc tgcgcgatca gatctttttc ctcgccgtcg
     5881 aacagcagtt gccgctgaac gggcaaaatg gcgcatccga taccatgctg cgcgccagcg
     5941 cctcattctt taatggcggc aaatacagcg acgaatggca cccgaacggt tcaggctggt
     6001 ttgcccaaaa cctgtacctc gatgcggcgc aatatatccg ccaggatatt caggcgtgga
     6061 cggcagatta tcgcgtcagc tggcatcaga aggtagctaa cggacagact attgagcctt
     6121 acgctcacgt tcaggacaac ggctatcgtg ataaaggcac tcagggcgcg cagcttggcg
     6181 gagtcggggt ccgctggaat atctggaccg gcgagacgca ctacgacgcc tggccgcaca
     6241 aagtcagtct cggcgtcgag tatcaacata cctttaaggc gattaatcaa cgtaacggag
     6301 agcgcaacaa cgcgtttctc accattggag tgcactggta aatgcgtaag ttcattttcg
     6361 tattgctgac actgcttttg gtcagccctt tttcctttgc gatgaaaggt attatctggc
     6421 aaccacaaaa ccgagatagt caggttaccg atacccagtg gcaggggctg atgagtcagt
     6481 tacgtttgca aggcttcgat accctggttt tgcaatggac ccgttacggc gatgcattta
     6541 cccagccaga acagcgcacg ttattgttta agcgtgccgc agctgcgcaa caggctggcc
     6601 tgaagctgat tgtcgggctg aacgccgatc cggaattttt tatgcaccag aaacagtcgt
     6661 ccgcagcgct ggaaagctat cttaatcgcc tgctggctgc cgatctccag caagccagat
     6721 tatggagcgc cgcgcctggc ataacgccgg atggctggta catcagcgcg gaaattgacg
     6781 acctgaactg gcgcagcgaa gctgcccatc agcctttgct aacatggtta aacaacgcgc
     6841 agcggctgat tagcgatgtt tcagcaaaac cggtttatat cagtagtttt ttcgccggaa
     6901 acatgtcgcc cgatggctat cgccaactgc tggaacacgt taaagcaacc ggcgttaatg
     6961 tttgggtaca ggatggcagc ggcgtggata aactgaccgc tgaacagcgt gaacgttatc
     7021 tacaagccag cgccgattgc caaagttccg ccccagcaag cggcattgtt tatgaacttt
     7081 ttgtcgccgg caaaggcaaa acctttacag cgaaaccgaa accggacgca gaaattgcct
     7141 cgctgttagc gaaacgttcc tcttgcggta aagacactct ctatttctct ctgcgctatt
     7201 tgcccgtcgc gcacggcatt ctcgagtatt aaatctcctc caggtaagtc gggtacgacc
     7261 tggcttacct ctttcgctct cgtcagaatt ccatcaagat taattgctaa aaagcgtgct
     7321 aaagaaagaa gatcgttatc ggcgaacggg ccgatcactc atgcaggaag catagatcct
     7381 ttgagcggag tttctgatat ctgaggagtg cgaaatgcaa ttgagcagca gtgaaccttg
     7441 cgtggtgatc ctgaccgaaa aagaggtaga ggtaagcgtc aataaccatg ctacgtttac
     7501 ccttccgaaa aactacctgg ccgccttcgc gtgcaacaat aacgtcattg aactctcaac
     7561 gttaaatcac gtattaatca cccacatcaa ccgtaacatc atcaacgatt atctgttgtt
     7621 tttaaataag aacttaacct gtgtaaagcc ctggtcgcgg ctggcaaccc cggttattgc
     7681 ttgtcatagc cgtacaccgg aagtgttccg gctagccgcc aaccacagca agcagcaacc
     7741 cagcaaaccc tgcgaagcgg agttgacgcg cgtattgctt tttaccgtat tgtctaactt
     7801 tcttgagcaa tcgcggttta ttgccctact gatgtatatc ttacgcagca gcgtccgcga
     7861 cagcgtctgc cgcattattc aaagcgatat tcagcattac tggaatctgc gaattgtcgc
     7921 cagttcgcta tgtttaagcc ccagcctgct caaaaagaaa ttaaaaaacg aaaataccag
     7981 ctatagccag attgtcacag agtgtcgtat gcgttacgcc gtacagatgt tattgatgga
     8041 taacaaaaat atcactcagg tggcgcaatt atgtggctat agcagcacgt catactttat
     8101 ctctgttttt aaggcgtttt acggcctgac accgttgaat tatctcgcca aacagcgaca
     8161 aaaagtgatg tggtgaaggg caaagcggaa acggataaga cgggcataaa tgaagaagaa
     8221 atggcgcgcc ctgcaggatt cgaacctgcg gcccacgact tagaaggtcg ttgctctatc
     8281 caactgagct aagggcgcgt tgataccgca atgcggtgta atcgcgtgaa ttatacggtc
     8341 aacccttgct gagtcaatgg cttttgatct ggttgctgaa caagtgaacg accgcgtctg
     8401 attttctgat ttatttcgct atagcggcaa acaaacacac accgctgcgc gtctgaatca
     8461 agaaaacccg tattttcatg tatcaaagta caatttaccg ccctaacgga aaattgtccg
     8521 ctcctatgag actggtaact atgaaaccaa cgtcggtgat cattatggat actcatccta
     8581 tcaccagaat gtctattgaa gttctgttgc aaaaaaacag tgaattgcag attgtcctga
     8641 aaacggatga ttaccgcata accatcgatt atctccgaac ccgtcctgtt gatttaatca
     8701 ttatggatat agacttgccc ggaacagacg gttttacctt cctgaaaagg atcaaacaaa
     8761 tccagagcac agtgaaagtg ttatttttat catcgaaatc agaatgcttt tatgctggca
     8821 gagcgataca agctggtgct aacggttttg tcagtaaatg caatgatcag aatgatattt
     8881 ttcatgccgt tcagatgatc ctctccggat acacgttttt tcccagcgaa acgcttaact
     8941 atataaaaag caataaatgt agtacgaata gttcaacggt cactgtgcta tctaatcgtg
     9001 aagtgaccat attacgttat ctggttagcg gatta
//
LOCUS       DAZFRN010000176         8980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFRN010000176 DAZFRN010000000
VERSION     DAZFRN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.178"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            17..1339
                     /locus_tag="ACHKUB_004286"
     CDS             17..1339
                     /locus_tag="ACHKUB_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO6657223.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFISMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1367..1678
                     /locus_tag="ACHKUB_004287"
     CDS             1367..1678
                     /locus_tag="ACHKUB_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO6657224.1"
                     /translation="MFADEELVMELLVNPGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1734..3407
                     /locus_tag="ACHKUB_004288"
     CDS             1734..3407
                     /locus_tag="ACHKUB_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6657225.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3432..4871
                     /locus_tag="ACHKUB_004289"
     CDS             3432..4871
                     /locus_tag="ACHKUB_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6657226.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4928..5146)
                     /gene="marB"
                     /locus_tag="ACHKUB_004290"
     CDS             complement(4928..5146)
                     /gene="marB"
                     /locus_tag="ACHKUB_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO6657227.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(5178..5561)
                     /gene="marA"
                     /locus_tag="ACHKUB_004291"
     CDS             complement(5178..5561)
                     /gene="marA"
                     /locus_tag="ACHKUB_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO6657228.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5581..6015)
                     /gene="marR"
                     /locus_tag="ACHKUB_004292"
     CDS             complement(5581..6015)
                     /gene="marR"
                     /locus_tag="ACHKUB_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO6657229.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6227..6892
                     /gene="marC"
                     /locus_tag="ACHKUB_004293"
     CDS             6227..6892
                     /gene="marC"
                     /locus_tag="ACHKUB_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO6657230.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6917..8107)
                     /gene="ydeA"
                     /locus_tag="ACHKUB_004294"
     CDS             complement(6917..8107)
                     /gene="ydeA"
                     /locus_tag="ACHKUB_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO6657231.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8257..>8980)
                     /gene="yneK"
                     /locus_tag="ACHKUB_004295"
     CDS             complement(8257..>8980)
                     /gene="yneK"
                     /locus_tag="ACHKUB_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO6657232.1"
                     /translation="PKPVTMGRVFQTGVERVLFLFLNDFIEQFPMINLGVPIKRAHTP
                     HIEPLPPDRHTAADYLRQFDLLVLNFISRGNFVILPRLWSNSEVHRWFVNKDPNLITA
                     ILDITDGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDFFLFFANML
                     LHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNR
                     PRSPVRPPIPATSKTPDLPERH"
BASE COUNT         2462 a         1920 c         2149 g         2449 t
ORIGIN      
        1 caacaggtat aaaaaaatgg gattaatggc ctcattcgaa cgtggaatgg aacgttttct
       61 tgttccagtt gctatcaagt taaactcaca aaaacatgtt gcagcggtga gagatggatt
      121 cgtttttacg tttccaatta tcatggcaag ttcattaatt atattaatta actttgccat
      181 attatcgcca gacggtttta ttgccggatt actgcatctg aatagcattt tccccaacct
      241 tgaaaaagca caagctattt ttactccggt aatgaatggt tctgtaaata tcatgtcaat
      301 tatgattgct ttcctggtcg ccaggaatat ggcgattagc tatgagcaag atgatctttt
      361 atgcggatta acggcaatag gagcattttt tattgtttat acgccatatc agatgataga
      421 tgggcaagca ttcctgacga ccaaatatct cggcgcgcag gggttgtttg ttgctgttat
      481 cgttgcattg atcaccagtg aaatattttg tcgcttagct cgaaacccaa aaatcaccat
      541 aacgatgccg gcagctgtac cgcctgcggt agcgcgttca tttaaagttt tattgccaat
      601 attttttgtc atggtgttct tttccgcact taattattgc ctgacactga tatccccggc
      661 aggactaaac gatcttattt acacattaat ccagacgccg ctcaaacata tgggaacgaa
      721 tatctttgcg gtaattatcc tgggggctgt gggtaatttc ctgtgggtgc tggggatcca
      781 cggacctaat accacatcgg caattcgaga aaccgttttt tctgaggcta atctggagaa
      841 tctctcctgg gccgctcaac atggcactac ctggggcgcg ccatatccga ttacctggac
      901 ttctattaat gatgcatttg ccaactgcgg cggttcaggt atgacgttgg ggttattgtt
      961 ggctattttt atcgcttcta agcgtgcgga ataccgtgat ctggcaaaaa tgtcatttat
     1021 ccccggtatt ttcaatatca atgaaccgat aatgtttggc cttcctattg tacttaaccc
     1081 catcatgatg gtgccgttta ttatggttcc cattgttaac tgtgccattg gttacttctt
     1141 tatttcgatg gaaattattc caccggttgc ttatgccgtg ccctggacta cgcccggacc
     1201 tttaattgct ttcctcggaa ccggggggaa ctggctggct ttactggttg gttttttatg
     1261 tttaggtgtg gcgacaatga tctatttacc ttttgttatt gccgccaaca aagtcaataa
     1321 catgacaact aacggataat tccatttacc caggaaggag attgtgatgt ttgcagatga
     1381 agaacttgtt atggagttac tggtcaaccc ggggcaggca cgttctgacg cgatggaagc
     1441 catacgttgt gccgggcaaa aagactggca gggcgcaact cagctaatgg ccagttctga
     1501 atctgcctgt cttcaggcac ataagattca gaccgcatta attagtcagg atgaaggctg
     1561 tggcaaaata aaagttaatt taattctgat tcatgcacag gatcatttga tgaatgcaat
     1621 attatgccag gatttagcca gggaaattat ttcattaaga aaagaactcc atgcctgatt
     1681 ttttcctcca gtgcgatagc tggaggaaat tattatactc ttaaggataa gtcatgaata
     1741 taaagacgct taacgtcagc cttttgtctt tttctattat tacagcattg tttccattga
     1801 acgcgatggc aacaaaatta accatagagc agcgccttga attgcttgaa aatgaattgt
     1861 cgcaaaataa acaagagctg aaagcaacac agaatgaact gggagtatat aaatcccgac
     1921 tttcgacctt acaaaaaagc atcacggaaa acaaatataa atcagcctcg cttgctgaaa
     1981 tatcagcgac atctcccgtc gctgataaca tcaaaaatga aaacggtgaa cagaactcgt
     2041 ctgccgcagc gcatactata aatggatcgc agcaagttgc cgttattgaa agtaaaggcg
     2101 acaaaaccac tatcgaaagc gtgaccctga aagatatcag taaatatata aaggatgata
     2161 ttgggttcag ctatcagggg tacttccgct caggctgggg tactggtaat cacggctcac
     2221 cacaaactta tgcagcgggt tctctgggac gttttggtaa tgagatgagc ggttggtttg
     2281 acctgacctt aaatcagcgt gtttataatc aggacggtaa aacggcaaat gcggtcgtta
     2341 cctatgatgg caacgtgggt gagcagtata acgatgcctg gtttggtgac agtgccaatg
     2401 aaaatatcat gcagttcagc gatatttacc tgacaacgcg aggtttttta cccttcgcac
     2461 cagaggcaga cttctgggtc ggcaaacata aactcccgca atatgagatc cagatgctgg
     2521 actggaaaac cttaaccacg gatgtcgccg cgggtgtggg gattgaaaac tgggcacttg
     2581 gtgtagggct gtttgatatg tccttaagcc gtgatgatgt cgatgtttac tctcgtgatt
     2641 tttcgcgtac cagccagatg aataccaatt ctgtggatgt tcgttatcgc aatatcccgt
     2701 tatgggatga tgcgacatta tcattaatgg ctaaatattc cgcacctaat aaaacggatc
     2761 aacaacaaga taacgaaaat gacaacagtt attttgaaat gaaagatagc tggatgctga
     2821 cttctgtttt acggcaaaac ctgcaacgcg atacgtttaa tgaatttacg ttacaggttg
     2881 ccaataattc ctatgccagc agttttgcca gtttctcaga tgccagtaat acgatggcgc
     2941 atggtcgcta ttactatggt gatcatacca atgggatcgc ctggcgttta atctctcagg
     3001 gcgagatgta tcttactgac aatattatta tggctaacgc gcttgtctat tctcacggcg
     3061 aagatgttta tagttatgaa agtggtgctc atagtgattt tgacagtatt cgcactgtaa
     3121 taagaccggc ctggatctgg aatacatgga atcagacggg gcttgaatta ggctggttta
     3181 agcaacagaa caaaactcag cagggtgtaa cactaaatga atcggcttat aaaacgacac
     3241 tctggcatgc attgaaagtg ggtgaaagca ttttaggttc acgaccagaa attcgcttct
     3301 atggaacgta tatcaatatt ctggataacg aattatcgaa ttttaagttt aatgagaaca
     3361 gcaaagacga gtttatggcc ggcatccagg cggaagtctg gtggtaatct attttagtta
     3421 aggagttgtg tatgtcagga tttaaaaaag gttttttatg gggcggtgcg gtagccgcgc
     3481 atcagttgga aggtggctgg aatgaaggag gaaaaggcat cagcatcgct gatgtgatga
     3541 ctgctggcgc tcacggggtg ccgcgtgaag tgacagaagg cgttatcgac gggcttaatt
     3601 atcccaatca tgaagcaatt gatttttatc atcgctataa aacagatatt cagttatttg
     3661 ccgagatggg attcaaatgc tttcgaactt ccattgcctg gacacgaatc tttccgcaag
     3721 gtgacgaaca ggagccgaat gaagagggtt tacaatttta tgatgatctg ttcgatgaat
     3781 gccttaagca gggaatggaa cctgtggtga cgctttcaca ttttgagatg ccttatcatc
     3841 tggtgacaaa atatggtggc tggcgaaacc gtaaactgat cgactttttt atccgcttcg
     3901 catcaacggt cttcacgcgc tataaagaaa aagtaaagta ctggatgacg tttaacgaaa
     3961 tcaataatca ggtgaatttc agcgaaagcc tgtgtccatt tactaattcc ggtatcttgt
     4021 attcgccaga ggaagatatc aatgagcgcg aacaaataat gtaccaggcg gtacattacg
     4081 agttagttgc cagtgccctg gcggtacaga ctggaaaatc gatcaatcct gaatttaaca
     4141 ttggctgtat gatcgccatg tgtcccatct atcctctgac gtgtgcaccc aacgatatga
     4201 tgatggccac gaaagcaatg catcgtcgtt actggtttac tgatgttcat gctcgtggat
     4261 attatccgca acatatgctg aattactttg ccaggaaagg attcaacctc gatatcacac
     4321 cagaagataa cgcgattctt gccagtggtt gtgtcgactt tatcggcttt agctactaca
     4381 tgtcttttac gacacaattt tcgcccgata acccgcaact ggattatgtt gaaccacgag
     4441 atttggtcag caacccttat atcgatacat ccgaatgggg atggcaaatt gatccggcag
     4501 ggctacgtta ttcactcaac tggttctggg atcatttcca gttgccgctg tttattgtcg
     4561 aaaatggatt tggtgcggtt gaccagagac aagctgacgg cacggtgaac gatcactatc
     4621 gcattgatta ctttgcttcc catattcggg aaatgaaaaa agccgtagtt gaagatggtg
     4681 ttgacttaat tggctacacc ccgtggggct gcattgacct ggtttctgcc ggaacagggg
     4741 aaatgaaaaa gcgctacgga atgatttatg tcgataaaga caacgaaggg aagggaacgc
     4801 tggaacggat acgtaaagcg tcgttttact ggtatcggga tctcatcgcc aacaatggcg
     4861 aaaatatttg agacttgagg aagatattac ggagatgtca ggctctgtga tcagagcctg
     4921 acaataacta catagcgtgt tgattataat agggcacgcc gagcgcatcc gacttatcac
     4981 tgccagtacc catgtgattt aaatcaaacg gtggttgttc ctgcgatggg ggaacaacca
     5041 cgacattggc acaagaagta acaactggct gcgtggtttg ttccgcaacg ccctgcgcgg
     5101 aaaagagaat aagcgcagct gctattgcgg atgaaagtgg tttcatgatt gcctcagtga
     5161 cgttgtcacg ttatcaacta gctgttgtaa tgatttaatg gatgtaaaaa gcgcgattcg
     5221 ccctgcatat tggtcatccg gtatttatgc ggcggaacat caaagtaatt tttgaaggtt
     5281 cgggtcagag tttgttgcga ctcgaagcca tatcgttctg ccagatagag tatcggctcg
     5341 ttactttcct tcagcttttg cgcgatttcc gtcatcttac ggctgcggat gtattggcct
     5401 aatgaatgac cggtttcttt tttaaacatc cgttgcaggt gccatttaga gtaacctgaa
     5461 cgctctgaca ctttctccag tgacagtggc gattccaggt tgtcctcgat ccagtccaaa
     5521 atgctatgaa tggtaatagc gtcagtattg cgtctggaca tcgtcatacc tcttttttgt
     5581 ttacggcagg actttcttaa gcaaatgctc aagtgttgcc acttcgtccg ccgtcaggtt
     5641 ttttgttaat tcttgatgca ggtcctggcc aactaattga tggcattgtt cgcatattgc
     5701 cgcgccgctg gtggtaagtt ttaccagtac gccgcgctta tcattcgggt tcggcaacct
     5761 ttcaacccag cctttacaga ccaggcgatc cagcatacgg gtcagtgctc caaggtcgac
     5821 agacaacact tttttcagtt caaccggagt aatacacgcc gcgcagcgga tagagcagag
     5881 caccttaaac tgtgccgcgg taatatccag cggagacaga tactcgttaa gcagacgatc
     5941 tttcttctga ttaaccatat ggattaagcg acccaatgga ataatttcat tgaacagatc
     6001 gctggtactt ttcacattag ttgccctggc aagtaattag ttgcagggga taatattgcc
     6061 caggcaagta taagtcaaat aaatgaattg accgatgcca cgttttgcta aatcggttca
     6121 agttttggtc aatgcgatgc aaggttagct aaagagtgac actttatcct tgtttacccc
     6181 ttatactttt cgctgataac ccagatacac aggataacaa ccactaatgt tagatttgtt
     6241 taaagcaatt ggcctggggc tggtggtgtt actgccgtta gctaacccat taacaactgt
     6301 cgcgttattt cttggcctgg caggcaacat gaacagtgcc gaacgtaatc gtcagtcgtt
     6361 gatggcctcg gtgtacgtat ttgccatcat gatggtggcg tattacgccg ggcaactggt
     6421 gatggataca tttggcattt cgattcccgg tctgcgaatt gcaggcggct taatcgtcgc
     6481 gtttatcggt tttcggatgc tctttccgca acagaaagcg attgattcac cggaggcgaa
     6541 aagcaagtcg gaagagctgg aagatgaacc cagtgccaat attgcctttg tgccgctagc
     6601 aatgccaagt accgctggtc cgggaaccat agcgatgatt attagctcgg cgtccacagt
     6661 gcgtcagagt tcaaccttcg ctgattgggt actgatggtt gcgccgccgc tgatcttttt
     6721 cctggtggcg gttattttgt ggggaagcct acgcagctcc ggcgcaatta tgcggctggt
     6781 gggcaagggt ggcattgaag cgatctcccg tctgatggga ttcctgctgg tatgtatggg
     6841 tgtgcagttt attattaacg gcatcctgga aatcattaaa acgtaccatt aaaaaaggcc
     6901 cgaatgggcc tttcaactat tgcgtctgtt cttcgagtgt cactggccag cggcgaaata
     6961 taatgattga ccaaattaac gcggcaaaag caggcaccgc gcccacataa ccaatcatcg
     7021 acattgacca gtgcaaactc acctgattac ctaccaacgc acccgccccg attccaatat
     7081 taaatatccc ggagaatagc gccatcgcga cgtcggtagc atctggtgcc agcgccagca
     7141 ctttaacctg cataccaagc ccgatgatca tcatcgcgat cccccagaaa atactcagca
     7201 ccccgaggtg tatttcactg ttcgccgcag gtaacagcaa tgccaggcac accagcaaca
     7261 gcgcaatcgc cgtactcacc aacgcagacg catactgatt acccagttta ccgaaaatca
     7321 cgctgccaat aatgcccgca ccaccgagta ataacagtaa tgccgtggca aagttggcgc
     7381 tgaatcccgc aatgttttgt acaaaaggct cgatatagct gtatgccgtg taatgggcgg
     7441 tgacaaccac cacagttaac aaataaatgc tcatcaatgc cgggcggcgg aacaatagcg
     7501 ggaggctttt cagtgaaccg gaatgctcac tgggcagtaa gggaagtaac ttaatcaggc
     7561 acaaaagggt gataagcgcc ccaataccaa tcgcgaagaa ggtcattcgc caaccgaaat
     7621 actggcccac aatgcgcccg agaggtaaac ctaagaccat cgccagtgct gtaccggtgg
     7681 caattaaact caatgcctgt gctcgcttcc cggccggagc catacggatc gccagagacg
     7741 ccgtaatcga ccagaaaatc gcatgtgcaa aagccacacc aatgcgactg atcaccagaa
     7801 cggtaaagct ccacgacaaa aacgacagta cgtggctggc aataaacacc acaaacaggc
     7861 agatcagtaa tttgcgccgt tcaacctgac tggtcattaa cataaaaggc aatgacatta
     7921 gcgctactac ccatgcgtaa atggtcaaca tgatgccgac ctgagcggtt tgcatgtgaa
     7981 aactttgcgc aatgtcagag agcaggccaa cagggacaaa ttcggtggtg ttgaagatga
     8041 aggcggcgac tgccagcgta acgacccgta gccacgccac tttgcgggaa acagtgtttg
     8101 ttgtcataac agattatcgg gtgagttgcg aaaaagtgat gaaatgatct taaaacggtt
     8161 aatgatgtga atacaatgca tttgtgatct ggatcgcgtt ttccgtttct ctgacgctga
     8221 ttctttcacg cgtcagagag tcggtaacat cacggtttaa tgacgttcag ggaggtctgg
     8281 cgttttggag gttgcgggta taggtggtct gacaggggag cgaggcctgt ttttcaccag
     8341 gtcttcgtga gtaacttttg gcggcgtttc attttttaca aacagtttga gggtcctgcg
     8401 cgccatatgt ttgataattt tatcatcact taatgatgct ggcatcaaca cgtcattcag
     8461 atcactttca ttgggattca taaattggtg atagtgcaat aacatattgg cgaaaaataa
     8521 aaaaaagtcc tgaaaatgag gagattcttg ttctgctaaa agggataaaa agcagatgca
     8581 gacttcgggt agtacatgtt tgttagaacc taatgaatcc atcaggcttt gcaataaatc
     8641 ctcttttaat tcgccgtctg ttatgtcgag aatggcggtg atcaagttag gatctttatt
     8701 gacgaaccat ctgtgaacct cagagttact ccataatcgg gggagtatga caaaattacc
     8761 gcgagagata aaattcagga caagcaaatc aaactggcgt aagtaatctg cagcggtatg
     8821 gcgatccgga ggcaggggtt caatatgtgg tgtatgcgct cttttgatgg gaacgccaag
     8881 gttgatcatt ggaaattgtt caataaaatc attcagaaac agaaatagca cacgctcgac
     8941 gccggtctga aatacgcgcc ccatagtgac aggctttggg
//
LOCUS       DAZFRN010000177         8957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFRN010000177 DAZFRN010000000
VERSION     DAZFRN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.179"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            60..332
                     /locus_tag="ACHKUB_004296"
     CDS             60..332
                     /locus_tag="ACHKUB_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO6657233.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(333..1205)
                     /gene="rluF"
                     /locus_tag="ACHKUB_004297"
     CDS             complement(333..1205)
                     /gene="rluF"
                     /locus_tag="ACHKUB_004297"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO6657234.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1417..2106
                     /gene="pepE"
                     /locus_tag="ACHKUB_004298"
     CDS             1417..2106
                     /gene="pepE"
                     /locus_tag="ACHKUB_004298"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO6657235.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2197..3828)
                     /locus_tag="ACHKUB_004299"
     CDS             complement(2197..3828)
                     /locus_tag="ACHKUB_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO6657236.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4048..7731)
                     /gene="metH"
                     /locus_tag="ACHKUB_004300"
     CDS             complement(4048..7731)
                     /gene="metH"
                     /locus_tag="ACHKUB_004300"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO6657237.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            7931..8755
                     /gene="iclR"
                     /locus_tag="ACHKUB_004301"
     CDS             7931..8755
                     /gene="iclR"
                     /locus_tag="ACHKUB_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO6657238.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2067 a         2478 c         2303 g         2109 t
ORIGIN      
        1 gttcagctcg caaacgaatt cccgtacact gaactctctg gctcactgag ggcaccatca
       61 tggcactccc ccgtattacc caaaaagaga tgactgaacg cgagcagcgt gaacttaaaa
      121 cactgctgga tcgggcgcgg attgcgcatg gtcgcgtact gaccaactcc gaaaccaaca
      181 gcattaagaa agagtacatc gataaattaa tggtcgagcg tgaggctgaa gcgaaaaaag
      241 cccgccagtt gaagaaaaag caggcttata aaccggatcc tgaagcgtca tttagctggt
      301 cagcaaatac gtcgacgcgc ggcaggcgct gattagcgcc ccttcttttt acgccccgga
      361 gaggtaaaac gcttaccgtt ggacgccggg cgaccgcctt tttccgccgt tttttccatc
      421 ttaacgactg gacgtttgat gcccgctgtt ttcggtttgg ccttcacttt aggttttgcc
      481 tcggaagagg aattttcgat gagcttaaag aggtcgatta actcatcatc agttaaatcg
      541 cgccactcac ccagcggaat gccgcttaag ctgacgttca taatgcgcgt acgttccagc
      601 tttttcactt catagccgaa atgctcgcac atgcgccgga tctgacggtt cagcccctgc
      661 accagggtaa tgcggaagac aaacggcgct tcttttttga ctttgcactt tttcgtcacc
      721 gtcccgagga tcggcacccc cgcccccata ccacgaataa attcatcggt aatcggttta
      781 tcgaccgtca ccagatactc tttctcatga tcgttacccg cacggaggat cttattcacc
      841 agatcgccgt gattggtgag gaaaatcagc ccctgggagt ctttatccag gcggccaatc
      901 gggaacacgc gtttgctgtg gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg
      961 gtggtgctga caataccgac gggcttgttc agggcgataa gtaccaaatc ttcggcttcc
     1021 cgaggctcaa tcaactgacc gtttactttc acaacgtcgc cgggtttcac ctgatcgcca
     1081 atggtggctc gcttgccatt aaggaacaca ttgccttgct cgatatagcg atccgcttcg
     1141 cggcgtgagc aaattccgct ttcgctgatg tatttattta aacggactga tgagtcgggc
     1201 agcataaatt ctcctgtaaa aaacggaata taccgtacct ttgggttgat aaaaaatagt
     1261 catgagcgaa ctatttgtta tttccattgg cttgaattcg ttacctgctc aaatcacttt
     1321 ccagaagcaa tgtgcgacat cgtgagccat gtcagtaagt ggttaaattc gcagtggtag
     1381 catgcctccc gtgatttctt actaaccgga gaagtaatgg aactgctttt attgagtaac
     1441 tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc tgaacagttg
     1501 cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac ctgggatgat
     1561 tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac cggtattcat
     1621 agcgttgtcg atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt cggcggcggg
     1681 aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggcacc aattactgac
     1741 gtggttaaac gtggcgctct gtatattggc tggagcgcag gcgctaacct tgcttgccca
     1801 accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga tgcgctaaat
     1861 ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg ccataaaggt
     1921 gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact gacgattatt
     1981 ggtctaccgg aaggtaactg gatcacagtg agtaaaggtc acgctacgct gggtggcccg
     2041 aacaccactt atgtgtttaa ggctggtgaa gaagcggtgc cgctagaagc tggtcaccgt
     2101 ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct gatgcgacgc
     2161 ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc gtccttcatc
     2221 ttcatccggc tgttccagca cactgtaagc caccgaacaa aacagcgagt tcaggcgctg
     2281 catatctccc agtaagccta aatgtagcga actggtttca atgctctgca cgttttgctg
     2341 atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa aacgatgttt
     2401 gctgcgacgc aaacgacgag cgctggtgac atcgccagag aagaacaccg acattgccag
     2461 ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc catcaagcga
     2521 aaatgcccgc cgtgctgcca gcgatttatc ggcaatttcg ctgcccatgc gctcgacgat
     2581 atcggaagcc tgttcaaggt tgagcgacat ttcaataatc tccgcccagc gacgcgactc
     2641 ttcttctgcc agttcctctt ttggcatccg cgccagatac agcttaatgg cggtatagag
     2701 aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct cgccgtgcat
     2761 cactttattc agcccttcca tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg
     2821 cgcggcgttc gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca gatgtttagg
     2881 ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa accgtgccat
     2941 cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga cgtggaaata
     3001 gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca gcaaatggac
     3061 aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca gcgcgacacg
     3121 gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt
     3181 agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg cggtcagagt
     3241 cgcagtcagc agtacagcag caaggctgga gtagctgata atggcgaaca tcgcgccaat
     3301 cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc
     3361 ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagtaaaa tcagcccaag
     3421 accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat
     3481 cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga gaatacgcgc
     3541 cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg cgagggctac
     3601 cagatcctga gcgacaaacg aggtcaccag catggtggtg gcattactgc tctgcaccag
     3661 tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag
     3721 gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa tatgagtccc
     3781 ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga gttcaggttc
     3841 tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa acacgctgac
     3901 tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt ccagcctgtt
     3961 tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg ccctgtttgt
     4021 tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat tcggtgccag
     4081 ccagcgctca acatcggaga cgctcatacc tttacggcgg gcatagtctt caacctgatc
     4141 gcgctgaatt tgcgccaccg cgtagtactt gctgtccggg tggctgaaat accaacccga
     4201 aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg tatgtttttc
     4261 cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg ccgggtagcc
     4321 cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc tgaggttctc
     4381 gttcggcgca tagccccagt agactttacg cacacgctca tggagatact ccgcaaaggc
     4441 ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat cgtgctgcgc
     4501 ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat
     4561 gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt agttagcgaa
     4621 tcccgttttt tcggtctgtt gacgcagatg gtggctgacg ttgatcacat gggtgcgcgt
     4681 ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca ggcccaccac
     4741 gccacgcgga ttcagcgttt tctcggcgct taatttatcc agcatgtcgt tggcgtcttt
     4801 aaacagccgc tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg gatacttccc
     4861 ggccagcgac caggtcataa agaacggtgt ccagtcgatg taattacgca gagtttcgat
     4921 gctggcttca acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt aagcctgcca
     4981 gtcaaaagcg aaatcgttat cgcgcgccgc ttccagcgtg accggtggtg tgcgcggttt
     5041 cttacgcccg tgctgaatac gtacggtttc gtactccttg cgggtacgag cgacaaaatc
     5101 atcacgctga gtatcggaaa gcagcgcagc caccacacca acggtgcgcg atgcgttctg
     5161 tacatacacc gtcgggccgc tgtagttctg ctcaattttc accgccgtgt gcgcttttga
     5221 ggtcgtcgcg ccgccaatca atagcgggat ggtgaagccc tgacgctcca tctctttcgc
     5281 cacgttaacc atctcgtcca gcgacggcgt gataagcccc gaaagtccaa tcagatcagc
     5341 attcacttct ttagcggtac ggagaatttt ttccgcaggc accataacgc cgagatcgac
     5401 aatttcgtag ttgttacatt gcagcaccac accaacgata tttttaccga tgtcgtggac
     5461 gtcgcccttc acggtggcga tcaccatctt gccgttggtt ttgccctgct ctttgctggc
     5521 ttcaataaac ggttcgagat acgccaccgc ctgtttcatg acgcgcgccg atttgaccac
     5581 ctgtggcagg aacattttcc cttcgccaaa cagatcgccg accacgttca tgccgtccat
     5641 caacgggcct tcaatcactt caatcgggcg cgtagcctgc tggcgggctt cttcggtatc
     5701 ctgctcgata aactcggtaa tgcctttgac cagcgagtat tccagacgtt tattcacttc
     5761 ccacgagcgc cactccgcct gctgggcgtt agcggtgtcg tcggttttgc tgccgcgata
     5821 tttctcggca agctccagta aacgctcagt gccatcatcg cgacgattaa gaatcacatc
     5881 ttccaccgcg tcgcgcagtt cagcgggcag gtcgtcgtaa atcgccagct gcccggcatt
     5941 gacgatcccc atatccatgc cattgcgaat agcgtagtag aggaacactg cgtgaatggc
     6001 ttcgcgcacc ggatcgttgc cacggaacga gaaagaaacg ttagatacgc cgccggaaat
     6061 tagcgcgtgc ggcagttcgc gtttgatgtc ttcacacgcg ccgataaagt cctgcgcgta
     6121 gttgttgtgc tcttcaatgc cggtggcaac cgcgaagatg ttcgggtcga agatgatatc
     6181 ttctggcggg aagccaacct cttcggtgag gattttgtac gcccgacggc aaatctcgat
     6241 tttccgtgcg cgggtatcgg cctgtccctg ttcgtcaaag gccattacca ccactgccgc
     6301 accgtaacga cgcaagagct tcgcgtgatg gataaaggcg tcgacgccct ctttcatcga
     6361 gatagagtta acaatgcctt tgccctggat acacttcaga cctttttcaa tgacatccca
     6421 ttttgaggag tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg caatcagatt
     6481 gagaaaacgc accatcgccg cttcggcgtc gagcatccct tcatccatgt tgatatcgat
     6541 aatctgcgca ccgttttcca cctgctggcg ggccacatcc agtgcctcgc tgtatttctc
     6601 ttctttgatc aggcgcttga acttagcgga accggtgacg ttggtgcgtt cacccacgtt
     6661 cacaaacagg ctatcttcgc caatgttcag cggctccagg ccggacaaac ggcaggctac
     6721 ggggatttcc ggcagtttgc gcggcgctaa tccttctact gcacgactca tcgctgcaat
     6781 atgttgtggc gtggtgccac agcagccgcc gacgatattg agaaaacccg cttgcgccca
     6841 ttcacgtatc tgttttgcca tcgtgtcggc gtcgagatcg tactcaccaa aggcgttggg
     6901 tagcccggcg ttcgggtgcg cggtgacgta gcattccgca atccgtgaca gctcctgcac
     6961 gtactggcgc agttcatctg gccccagcgc acagttcagg ccaaaggtca gagcttcggc
     7021 gtggcgcaat gagttgtaaa atgcttcggt ggtctgcccg gagagcgtgc gcccggaggc
     7081 gtcggtgatg gtgccggaga tcataatcgg cagctcaacg cccagcgctt caaactccgt
     7141 tttcaccgca aagaccgccg ctttggcgtt aagggtgtcg aaaacggttt caatcaggat
     7201 cagatccgca ccaccttcca ccagcgcttt ggtggactct cgataagccg ccaccagccc
     7261 gtcaaaagtg atattacgaa atgccggatc gttgacgtcc ggagaaatag acgccgtgcg
     7321 gttggttggg ccgagaacac cggcaacgta gcgcggtttc tccggggtgc gcgcggtcca
     7381 ctcatcagca caggcgcgcg ccagtttcgc cgccgcaaag ttgatttccg ccgacaggga
     7441 ttccatctgg taatccgcca tcgcaatggt cgtggagttg aaggtgttgg tttcgatgat
     7501 atccgcgccc gcttcaaagt aggcgttgtg gatagcggcg atcacttccg gtttactgag
     7561 taccagcagg tcgttgttgc ctttgaggtc gcatggccag tcggcaaagc gttcaccacg
     7621 aaaatcggct tcgttcagtc gatagctctg gatcatggtg cccataccgc cgtccagcac
     7681 cagaatacgt tcatttaact gcgcacgcag ttgttccact ttgctgctca cacttgctcc
     7741 cgacacgctc aacccagatt taataaaaat tcaacaaacc atactggcat aaacgcatct
     7801 gtggtaaaag cgaccaccac gcaacatgag atttgttcaa cattaactca tcggatcagt
     7861 tcagcaacta ttgcattagc taacaataaa aatgaaaatg atttccacga tacagaaaaa
     7921 ggagactgtc atggtcgcac ccattcccgc gaaacgcggc agaaaacccg ccgttgccac
     7981 cgcaccagcg actggacagg ttcagtcttt aacgcgtggc ctgaaattac tggagtggat
     8041 tgccgaatcc aatggcagtg tggcactcac ggaactggcg caacaagccg ggttacccaa
     8101 ttccacgacc caccgcctgc taaccacgat gcaacagcag ggtttcgtgc gtcaggttgg
     8161 cgaactggga cactgggcaa tcggcgcaca tgcctttatg gtcggcagca gctttctcca
     8221 gagccgtaat ttgttagcaa ttgttcaccc tatcctgcgc aatctaatgg aagagtctgg
     8281 cgaaacggtc aatatggcgg tgcttgatca aagcgatcac gaagcgatta ttatcgacca
     8341 ggtacagtgt acccacctga tgcgaatgtc cgcgcctatc ggcggtaaat tgccgatgca
     8401 cgcttccggt gcgggtaaag cctttttagc ccaactgagc gaagaacagg tgacgaagct
     8461 gctgcaccgc aaagggttac atgcctatac ccacgcaacg ctggtgtctc ctgtgcattt
     8521 aaaagaagat ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg aggaacatgc
     8581 gctggggcta cgttgccttg cagcgtgtat tttcgatgag caccgcgaac cgtttgccgc
     8641 aatttcaatt tccggaccga tttcacgtat taccgatgac cgcgtgaccg agtttggcgc
     8701 aatggtgatt aaagcggcga aggaagtgac gctggcgtac ggtggaatgc gctgactttt
     8761 ttctggcggg cagaggcaat attctgcccg tcatatctga gtggcaatag aataagggtg
     8821 tctgttaatc gcattgacgc caaaataact tattgtcata cacttcgcac acttttatta
     8881 actcagcatt atttttaaac atcaaaccac ttaattatta acaaaatcgc aagatctcat
     8941 tacgccgaaa cttttac
//
LOCUS       DAZFRN010000178         8915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFRN010000178 DAZFRN010000000
VERSION     DAZFRN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.180"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..2150)
                     /gene="traC"
                     /locus_tag="ACHKUB_004302"
     CDS             complement(<1..2150)
                     /gene="traC"
                     /locus_tag="ACHKUB_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO6657239.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEILGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTLGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAETQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLALVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKG"
     gene            complement(2277..2624)
                     /locus_tag="ACHKUB_004303"
     CDS             complement(2277..2624)
                     /locus_tag="ACHKUB_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657240.1"
                     /translation="MKPIYLKYFLSFVFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTIGKANKIASECRKRNEMAEARRVRENERNKIREKQ
                     LQALNDAHSVKER"
     gene            complement(2809..3027)
                     /locus_tag="ACHKUB_004304"
     CDS             complement(2809..3027)
                     /locus_tag="ACHKUB_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657241.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVQTNEPLNNVMETLNGGGQ"
     gene            complement(3107..3583)
                     /locus_tag="ACHKUB_004305"
     CDS             complement(3107..3583)
                     /locus_tag="ACHKUB_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657242.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRRVLSRQELLWLMSLTEDCPDM
                     HQELLNLLLSGKKLTGLDKREIRSLWWEKMDAMQESATRQREQDTIRQFSEESKSE"
     gene            complement(3576..3797)
                     /gene="traR"
                     /locus_tag="ACHKUB_004306"
     CDS             complement(3576..3797)
                     /gene="traR"
                     /locus_tag="ACHKUB_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO6657243.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(3932..4447)
                     /gene="traV"
                     /locus_tag="ACHKUB_004307"
     CDS             complement(3932..4447)
                     /gene="traV"
                     /locus_tag="ACHKUB_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO6657244.1"
                     /translation="MKKISLFVPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKMEQPADAKPVAASLPRLADGNFRTTSVQAVAATTPSRSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVVPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(4444..4716)
                     /locus_tag="ACHKUB_004308"
     CDS             complement(4444..4716)
                     /locus_tag="ACHKUB_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO6657245.1"
                     /translation="MFISLMTEQSEITEIKMNMRNLNIIKSSPQSLPHNFNIAAVGLM
                     KKGMSMSLFSSPGHIMVVGQTCAGKANLTAELISAVVGNNKKGEGQ"
     gene            complement(4655..5215)
                     /gene="traP"
                     /locus_tag="ACHKUB_004309"
     CDS             complement(4655..5215)
                     /gene="traP"
                     /locus_tag="ACHKUB_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO6657246.1"
                     /translation="MVGDALIFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVQEIDFVRQTAPAGQFPVRECLPSLHDLPGGAEKICRYRAADAADYVRETD
                     RSLMQLTTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVAAADERLKGVYTEDASLP
                     EKIRKCPVYFPDDRAIRNNGDKNEHA"
     gene            complement(5205..6659)
                     /gene="traB"
                     /locus_tag="ACHKUB_004310"
     CDS             complement(5205..6659)
                     /gene="traB"
                     /locus_tag="ACHKUB_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO6657247.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGTGAVPPPTAFYPGNGTTPPPQVTYQSVPVPNRIQRKTFSYDADRKKGPSLPYIPSG
                     SFAKSVLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGVTAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQSQSQAEEGNAESPVPNPPTAESHLNGFNTD
                     QMLKQLGNLNPQQFMSGDRNQQGGDAGKEGGNGG"
     gene            complement(6659..7387)
                     /gene="traK"
                     /locus_tag="ACHKUB_004311"
     CDS             complement(6659..7387)
                     /gene="traK"
                     /locus_tag="ACHKUB_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO6657248.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTLSALNIRESDFWQSGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(7374..7940)
                     /gene="traE"
                     /locus_tag="ACHKUB_004312"
     CDS             complement(7374..7940)
                     /gene="traE"
                     /locus_tag="ACHKUB_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO6657249.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLAVNVIQGINNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(7962..8273)
                     /gene="traL"
                     /locus_tag="ACHKUB_004313"
     CDS             complement(7962..8273)
                     /gene="traL"
                     /locus_tag="ACHKUB_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO6657250.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAGICIGWGMMT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(8288..8653)
                     /gene="traA"
                     /locus_tag="ACHKUB_004314"
     CDS             complement(8288..8653)
                     /gene="traA"
                     /locus_tag="ACHKUB_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO6657251.1"
                     /translation="MNAILSVQGASAPVKKKSFFPKFTRLNMLRLVRAVIPAAVLMMF
                     FPQLALAAQGAGQDLMAGGNATVKATFGKDSSAVKWVILAEVLVGAIMYMMTKNVKFL
                     AGFAIISVFITVGMAVVGL"
     gene            complement(8686..8913)
                     /gene="traY"
                     /locus_tag="ACHKUB_004315"
     CDS             complement(8686..8913)
                     /gene="traY"
                     /locus_tag="ACHKUB_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO6657252.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLSRFPDFYNLDAIKEGAGETDSIIKDL"
BASE COUNT         2151 a         2454 c         2066 g         2244 t
ORIGIN      
        1 cctttctcaa taaactcacc gaccttgtga tttttgaagt ccagcagacg ccagccttca
       61 tcaataacgt tcagtttctt gaggttacgc ggcgtgcggt acatccggtt ctcgatgtaa
      121 atgataaggc tgaacatcac cgcaaccagc agggacggac ggtcttccag tccacccagc
      181 tccagcacca ccattttcgc atcatcccgc agggacggtt catcagagtt aaaatactgg
      241 ccgtaagtac cgttggcagt gtactggtcc agcagcacta tcatttcatc cagacggctg
      301 cggatggtcg gggactcggc atactggtcg ttatcccggg cgtttttcag gaaatccacc
      361 acatcatcaa tacgcgcccg gttctgtctg gtaagccagg aggccctgac cgcctgcagc
      421 agcagacctt catgcacttc atccaggtta ccgttggggc tggccatcac cgacaactgg
      481 tcacggacac gctccgctga ctggtcaata tcggtgatgt tcgcaaacgg attaaagcgc
      541 agggtttcac cgtccagata tacgcccccc atgttctcac acagggactt gtagccatcc
      601 cccatgtcga acaccacggc aaagcccccg gagtccagca cgctgcggat aagtggctgt
      661 atcagcccgg ttttaccggc cccggaggtg ccacagaccg ccatgttgta gttggtgtta
      721 ttcatccccc ggaagaaaat atcgataaac gccagctggt tacggtaggt gggtgccagc
      781 agacctgccg gtgtcagggg gttatccgcc actaacggca tcaggttggc cacattaaag
      841 ctctctgcgc gctgaaccac accggcctct ttcagctgtt taaacagccc tttcccggcc
      901 ataaagggca ggcaggtcag aaaattgcgc atgtggttaa agcgcggtga aatcagctca
      961 aaaccgtttt tacggaagct gttcaggatg tcctgctcca cttccagcgc cgtttcattg
     1021 ttgtccttgc agaacgctgt gatgttgagg aagtaggaca ccacggagga ctgacctgag
     1081 cccagccgct ggcgcagttc cccccactcc ttcgcttctt tctccacgga cggaaaccat
     1141 ttggcatagg aggtcttcga cttcttctcc aggtccatgt acttcaggtt ggcttcgctg
     1201 tgggttttca cctggtcttc caccaccagg gccagcgtca ggatgaacgg gcaggaaatg
     1261 gacatttccg ggttcagcag gttgctgtag ttgtcggcca tgttccacag gaaggcgatt
     1321 tccgggttac gggccagatg gaaattcagg atgcgggccg tgctgttcct gccgttctca
     1381 cgcaggccca gcgtcaggta atcagcccgg actttcagat caaaactgtc ctccacacac
     1441 tgataattca aatcagaata cgggtccagc tgacgtcttt tcgggtacag ggagtccggg
     1501 ttatggttaa tcatctcccc gacaatatcg atgaaagcct gtgcatccac cgtctgcgtg
     1561 gtgatatatg ctccctgcag agacgcccgg atgattttca ccaggttttc catttccaga
     1621 atgtcggccc ggcttttttt cttcgaggga gaacagtacg agataaacac ccggtaatgg
     1681 cgcagggtca ggggcagatt cattccctcc ggcagcggaa actgtgtttc tgcggctttc
     1741 atataatagg cacgggtgat tgcgttaaac cgctcagcct gctcaccgga ccaggagaac
     1801 tcccgcaggc cgtattcaat cctgtcacca accagctgac tggacatcag atggatacac
     1861 aacggaatac cgcgcggcag tttggtacgc agcatgtggt cgagcgcctc cacaatggac
     1921 tcattcgccc cattaatggg aatggcttcc agcataaagc ccagcgtggt gtcattcatg
     1981 aacagaccag attcctggtt gtaatcccgg tacggcagca gacggctgaa ctgcgggaag
     2041 ctcatttcac ccagtatttc attggccttt gcggattcat caggcagttt cagtgcggtg
     2101 acaagggagt tcaccgcctg agtgacggcc tcaagtgggt tattactcat acgagtattc
     2161 cttagtacgt ttaaaaaagg aaaatatcag tctgtggccg tggatatatc acgcagtgcc
     2221 attcttaaaa ccacagcaca aaataagaaa tggaggcttt taacaggaaa ccaggattaa
     2281 cgttccttta ctgaatgagc atcatttaat gcctgaagtt gtttttcgcg tattttattg
     2341 cgctcatttt ctcttacccg tcgcgcctct gccatttcat ttctttttct gcattctgat
     2401 gcgattttat ttgccttacc aatggttaaa ggttcagact gataattaag tgtctcctga
     2461 aatgcttctg ctgcacaggg agtttcctgt aaaatctctg tcagttgttc tggtgttgca
     2521 ggttgttgcg caaggtaaac gatgtaaaaa ggaatacata caatcatagc cataacacca
     2581 acaaatacaa aagacagaaa atattttaaa tatatcggct tcatttaatc ttagccttat
     2641 atagtccgat aagtacgcta acaatcaatg tgttacctct gttgactcgc agactgaatt
     2701 tttcagggat aacaaacata gccagtgcgt taacaacaca gataaccaca agaaagaggc
     2761 acaggcatat aaaccagtca atattatttc cttcgaagca gtaagcactc attgcccacc
     2821 accattcagt gtttccatca cattgttcaa aggttcattt gtttgaactt caatacattc
     2881 tcctgtattt ccaaagcccc tgcctccatg cgcttcgatt gtccgaagaa cctgcacgca
     2941 tacatccgta tttggcatgg gtgtaacatt ccagcttccg ccaccgttaa tattttttat
     3001 cagcacaaga aaaataaagg tatgcattct ttattacctc cagtgaaata ttaatcactg
     3061 aatattcctt cagttaactt atgaggttat aaaacacagc agattttcat tcggatttac
     3121 tctcttcaga aaactgccgg atggtatcct gttcacgctg acgagtggca gactcctgca
     3181 ttgcatccat cttttcccac cagagactcc tgatttccct cttgtccaga ccggtcagtt
     3241 ttttaccgga cagcaggagg ttaagcagtt cctggtgcat atccggacag tcttcagtca
     3301 gactcataag ccagagcaat tcctgacgac tgagtaccct gcgtgattcc tccggtacgc
     3361 tgtaataatt aaaaatacga ctgaataccc cacaacagca ggcaacggac aggcacaggg
     3421 atttcagata caggccagaa acacagaatg tggcaacacc acaaagaata gctataacta
     3481 aaaagccctt acccagtgcc atacacaaag aaccctgttt tctgaatatc cgctcatgct
     3541 catccagaat aaaatctatt ttttcatttt ctgatttatg catagtgttt cctctgcctt
     3601 tcctgatacg cctgacattc aacacataac gtcacaccgg gaaatatttt ccgccgggct
     3661 tccggaatag gatttccgca tgcttcacag agataaacag gaataccatg agcatttatt
     3721 ttctggcgta tccggtttat tcctgtcatg gtcagttgtt ctgtcactga atatgcttca
     3781 tcggcttcat cactcaagga atattcctcc cgacatgttc tgacacgcta ccgcccatcc
     3841 cttttcacgc acagggcact cgttttacag gcagattttg aatacagaaa gcccgccggc
     3901 attgccggct gatatacaac ttcagggcct gttaattaat acgtggtttt ccccacgcag
     3961 acggtttaat aacaaaaaac acgctggatg gctgatgata aacatcctga ttatcaatat
     4021 acggcgctat ccataatgcc gccgtctgtt cacccgttct tagcggacgt gggggtgtta
     4081 ccggcacaac tgaactgacc ggaacaaccg ttttcacttc ccgggcagcg gtaaacagcg
     4141 ggcgtggtgc cagcagtttc tgctccggaa gtgctgtcac agcaggcctg cttcgggacg
     4201 gcgttgtcgc cgcaacggcc tgcaccgacg tagtcctgaa gttcccgtca gccaggcgcg
     4261 gcagtgatgc cgcaaccggc tttgcatctg caggctgttc catctttttg gctttttcgt
     4321 tggcctgttc cattgtcatg caggtatctg acgtggtggc atcacactca aaatcagagt
     4381 tcgttccggc gcagccggaa agtaataagg ttcccagcag aggaacaaat aaagaaatct
     4441 ttttcattgc ccttctcctt ttttattatt ccccacaaca gcagaaatta actctgcagt
     4501 cagatttgct ttccccgcac aggtttgtcc gacaaccata atatgccccg gagaagaaaa
     4561 taaactcatc gacatcccct tcttcatcaa accgacagcg gctatattaa aattgtgcgg
     4621 cagtgattga ggactgcttt taataatatt aagattacgc atgttcattt ttatctccgt
     4681 tatttctgat tgctctgtca tcagggaaat aaacagggca tttccttatt ttttccggca
     4741 gggaagcgtc ttccgtataa actcccttca gacgttcatc tgcggctgcc acccggacac
     4801 atttcatttt tccgggccac atcggatatt tcccggtgcc tgccgcaaac gacatataca
     4861 tcagtgccag tgttgcccag aatgcggtgg tcagttgcat cagtgagcgg tcagtctccc
     4921 tgacataatc tgcagcatca gcagcacggt aacggcagat tttctctgct ccccccggaa
     4981 ggtcatgcag agaaggcaga cattctctta caggaaactg cccggcaggg gctgtttgtc
     5041 ttacgaagtc aatttcctga accagtaatt ttcctggtgt ggtgttgtcc ttccataaaa
     5101 caaataacgc catcagggcc attgtcgcca gcggccagat aaccacaaac ctgacgaccc
     5161 acagcagcca gcgaacgaca aacgccacac ggaatatcag tgcatcaccc accatttccg
     5221 ccctccttac cggcatcccc gccctgctga ttcctgtctc cggacataaa ctgctgcgga
     5281 ttcaggttgc ccagctgttt cagcatctgg tcggtgttaa aaccgttcag gtggctttct
     5341 gccgtgggcg gattcggcac cgggctttca gcgttcccct cctccgcctg gctctgtgac
     5401 tgctccagcg ccatctcctc cacggttttc agctggaagc catcctggaa caccaccgtc
     5461 acttcattgc ccgcaccaat cggtatcacc gggtgatact gctcagcgcg tttgatgtag
     5521 tagtcactga gtgtctgtgc cgcttttgat gcaccgccac cgatacccat tttcaggaca
     5581 tcgccggctc cgtaagcggc agtgactccc agcccgacag caggctgtga agctcgctcc
     5641 attccctgac cgataccgtc aacaaatccc gcgccccatg cccagccgag gattttgccg
     5701 ttacgcatca ccacttcgcc cttgataccg tttttacccc ggaagctgac atgcccctta
     5761 atcggcatat caatagtttt gccgtccttc aggcagctga tattgcgggt gcgtacaatg
     5821 gcacgctcac tggacacatc cccccaggct tccagaccca caaaacatcc cgtggcgtca
     5881 tacgttttgc tgttcggcat ttccaccagg ccggtgatac gcagctgcat cggcacagtg
     5941 gattcattac cggtgacaga cgcattggca tctgccccct caatcaggac ggattttgca
     6001 aagctcccgg acgggatata cggcagggag gggcccttct ttctgtcagc gtcatagctg
     6061 aacgttttac gctgaatacg gttcggtacc ggcacagact ggtatgtcac ctgcggaggc
     6121 ggtgtcgtgc cgttccccgg ataaaacgcc gtcggcggtg gaacagcacc ggtgccctgc
     6181 ggagggaaag atacgggtgt gtttcctggc tgtggttcgc cttccggccc gggcggtgag
     6241 gctggcgtct gcggtacagg ctcaccggtc gcggtgacgg gatttgcacc cagggctttt
     6301 acctgctctg ccagggcggc attgtcctgt cccagctttt caatacgacg ctggtcatca
     6361 ccacgctgtt tgttcaggac atccagttca cgacggattt cctcatactg cttctgcatc
     6421 tgcgctgccg tcacctgcat ctctgtggtg gcatgctgac gcaccttgtc atcaaaggtc
     6481 gtatccacca caccggtcat atccggcaca ggctcctgtt cagccacggt ttcaccgtta
     6541 ccggacatgt ccacatcaga cagatacagt gccccaccaa tcgcggaggc tgtaccgaca
     6601 accacaatcc ccagccacag gtactgcttg cgtttcacaa tggtattgat actggccatc
     6661 agttgccctc cccgtcacgg atgacataca catccatgcg cgcacctgcc agtaactgac
     6721 tggcaggctg gctgaacatc acggcacggg ttcccgactg ccagaaatca ctttcccgga
     6781 tattcagggc agacagcgtt ttattttcca cgacaaagcg gaccatcttc aggtgattcc
     6841 ccgtccatac ggaatctgcc actgaagaca gccccgccgg ggcctgcagg gtttcctttg
     6901 tcacaggaac ctgataccag ccggcgggta attttccgcc acggacagcc tggctgatgg
     6961 tcacaagcag ggattcgtaa ggcgtggacg tttcccacgc gccggcttct tctccggttc
     7021 cgcgcaggtc actgaccagc tgaatggtac gccctgcgcc ttcacgggga acggcctgaa
     7081 tggaaaggtt aagaccacgt tctgtttcca gaatgaacgt aaaaggcttt ttgttgacgg
     7141 tggccaccac cacaccgccg gaggcggtct gctcattatt ggtcagggca ccatccagac
     7201 tgttcacggc gataatccgg tcaccaggaa cggtaaacag attcgggctg gtattgctga
     7261 tggcaatact ggcctgacca ccgttcacca ttggcaccac ggtaggggcc agcgtgccgt
     7321 ttgcggccat cacgctgcag gaaaaaaaca ggccgccgaa tattattgct gtattatttt
     7381 ttctcatcgt ctgtttcccc gaaattatcc agccaggtca ccccgttttc ccgcttcaga
     7441 ataaggatgt aatgtttgat atcagtaaaa ggttcggagt taccaatcca tgttttccga
     7501 attccacgga tttccacacg accatattgt ggccagacac ggacactggt ctggaaaaaa
     7561 gcagaattca cgttatcttt tttaatacgc ttcgcttctt cagccagaat aactttcatc
     7621 tgattctgtg cgcccggacg gatatactgc agaagcgcct gatgtgaggc atcgacgatt
     7681 tccggtgaaa cattcagacg aagggcaata aatgccagtg ccatctgctg taaataagag
     7741 gcatcggcac tgttctgtga cacggcaaag ggtacattaa atcccatcgg ggtcacggca
     7801 gtgcgttgct cattctgaag acggtagtta tttatcccct gaatgacgtt aacggcgagg
     7861 ctgaggacaa taagcaccga cataaatata aaggcgatgg ccattacacg actggtactt
     7921 aaacgggcac cgtgttccat agaataatcc tggtatcagt tctatttaat ccactgccgg
     7981 aaacacgaat cgggaacatt atgaaaaata ccgcgcaaca ggactgttgg catataccag
     8041 taaatcaggt cacgtaacca gaagctgccc cgcccttttt tcagtttttt aatcccgaaa
     8101 taaaccagaa ccgctgcacc aataccaaac agatattttg atgtcatcat tccccagcca
     8161 atacaaattc cggcaggaat aagttcatcc agcggcaggc caaaccagcg gctctggttg
     8221 gtcagtgttt ccgggaaacg atattttttg agtttattct cgtctcccga catgacgtta
     8281 tttcctgtca gagaccgacg actgccatac ccacggtaat aaacacggag ataatggcaa
     8341 aaccggcgag gaacttaacg tttttggtca tcatgtacat gatggccccg acgagaactt
     8401 ccgccagaat cacccatttc accgcactgg agtctttacc gaaggtggcc ttgacggtgg
     8461 cgttaccccc ggccatcagg tcctgacctg ccccctgggc ggccagtgcc agttgcggga
     8521 agaacatcat cagaacagca gccgggatca ctgcacgaac caggcgaagc atattcagac
     8581 gcgtgaattt aggaaaaaac gacttctttt tgacgggcgc agaagcaccc tgaacactca
     8641 aaatagcatt cataagttac ctctattgtg gatagattaa taaacttaaa gatctttaat
     8701 tatcgaatca gtttcccctg ctccctcctt tattgcatcc agattataaa agtcaggaaa
     8761 acggctcaga tgatcacgta acctgactag cacctcatta gtttttgttc tcccgctacg
     8821 ctcccgggca cctaagagct tgtgattagt agcatcgtcc aataccagta aaactttatt
     8881 gcccggagca ggccttatta tatttctgct caagt
//
LOCUS       DAZFRN010000179         8909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFRN010000179 DAZFRN010000000
VERSION     DAZFRN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.181"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1031
                     /locus_tag="ACHKUB_004316"
     CDS             <1..1031
                     /locus_tag="ACHKUB_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6657253.1"
                     /translation="TLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKK
                     TGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAF
                     LLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFV
                     VSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQ
                     FKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIA
                     ERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGG
                     NKVIIHHI"
     gene            1212..2702
                     /gene="dgcJ"
                     /locus_tag="ACHKUB_004317"
     CDS             1212..2702
                     /gene="dgcJ"
                     /locus_tag="ACHKUB_004317"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO6657254.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2745..3248
                     /gene="yeaK"
                     /locus_tag="ACHKUB_004318"
     CDS             2745..3248
                     /gene="yeaK"
                     /locus_tag="ACHKUB_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO6657255.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3249..3353)
                     /gene="yoaI"
                     /locus_tag="ACHKUB_004319"
     CDS             complement(3249..3353)
                     /gene="yoaI"
                     /locus_tag="ACHKUB_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO6657256.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3523..3969
                     /gene="yeaL"
                     /locus_tag="ACHKUB_004320"
     CDS             3523..3969
                     /gene="yeaL"
                     /locus_tag="ACHKUB_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO6657257.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3926..4747)
                     /gene="nimR"
                     /locus_tag="ACHKUB_004321"
     CDS             complement(3926..4747)
                     /gene="nimR"
                     /locus_tag="ACHKUB_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO6657258.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKQDPRQ"
     gene            4844..6025
                     /gene="nimT"
                     /locus_tag="ACHKUB_004322"
     CDS             4844..6025
                     /gene="nimT"
                     /locus_tag="ACHKUB_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO6657259.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6080..6427
                     /gene="yeaO"
                     /locus_tag="ACHKUB_004323"
     CDS             6080..6427
                     /gene="yeaO"
                     /locus_tag="ACHKUB_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO6657260.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6449..6703)
                     /gene="yoaF"
                     /locus_tag="ACHKUB_004324"
     CDS             complement(6449..6703)
                     /gene="yoaF"
                     /locus_tag="ACHKUB_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO6657261.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6886..7911
                     /gene="dgcP"
                     /locus_tag="ACHKUB_004325"
     CDS             6886..7911
                     /gene="dgcP"
                     /locus_tag="ACHKUB_004325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO6657262.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGEHLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            7944..8042
                     /gene="yoaK"
                     /locus_tag="ACHKUB_004326"
     CDS             7944..8042
                     /gene="yoaK"
                     /locus_tag="ACHKUB_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO6657263.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8045..8119
                     /gene="yoaJ"
                     /locus_tag="ACHKUB_004327"
     CDS             8045..8119
                     /gene="yoaJ"
                     /locus_tag="ACHKUB_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO6657264.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8178..8426)
                     /gene="yeaQ"
                     /locus_tag="ACHKUB_004328"
     CDS             complement(8178..8426)
                     /gene="yeaQ"
                     /locus_tag="ACHKUB_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6657265.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8653..>8909)
                     /locus_tag="ACHKUB_004329"
     CDS             complement(8653..>8909)
                     /locus_tag="ACHKUB_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6657266.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         2196 a         2153 c         2109 g         2451 t
ORIGIN      
        1 gtacactgat ccagacaaag tcgaatgatg tttctatttt ctatattttc cgccaactca
       61 gttttatttg tttaacctcg ctggcactct tttgttatgg aaaagacaac atccttgaca
      121 acaataagaa aaaaacggga atcctgttgc tggcgctgat tcctttttta gtttttcccc
      181 ttctggcaca caatctgagc agttataacg ctgactattc tttgtatgtc gtcgactact
      241 gtccggacaa ccatactgcg acctggggaa tcaactatac aaaaatattg gtttgtctgt
      301 gggcattttt actgttcttt attatcatgc gtacacgatt agccagcgaa ctatggccgt
      361 taatagcatt attatgtctg gcatcgctat gctgcaactt acttctactg actctggatg
      421 agtataatta caccatctgg tatatcagtc gcgggattga agtttccagt aaactgtttg
      481 ttgtgtcttt tctgatttat aacatttttc aggaactgca actctccagc aaactggcag
      541 ttcatgatgt gctgaccaat atttataatc ggcgctactt tttcaacagc gtagagtcat
      601 tattgtcgcg acctgttgtt aaggacttct gtgtcatgct ggttgatatt aatcagttca
      661 aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa agtgctggtt tcaattgtcg
      721 atattatcca gcaaagcatc cgccctgatg atattttagc gcgactggag ggtgaggtgt
      781 ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa aatcattgcg gaacgtatgc
      841 gtaaaaatgt cgaactcctg accggcttta gtaacagata tgatgttcct gaacaaatga
      901 ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg taatatctcg cttgtcatga
      961 cggaagcaga taaagcctta cgcgaagcga aaagcgaggg gggcaacaaa gtgattattc
     1021 atcatattta agtgcaaaaa tattcagagc catgcttttt gcgtggcttt tgcatacaat
     1081 ttattactat tacccctaaa tttcccctcc ccagcggcgc agagatgagt ataattagcg
     1141 cccctgtgcc aggccgcaat cgaactttat ctggttttct cgtttcacta accgaaggag
     1201 tgccatttat catgaaattg caccatagaa tgctccggca ttttatcgcc gcaagtgtca
     1261 ttgtgctgac atcttccttc cttatttttg aacttgtcgc cagcgacaga gcaatgagtg
     1321 cctatctgcg ctatatcgtg cagaaagcag actcctcctt tctttatgat aagtatcaga
     1381 atcagagtat tgccgcgcat gtgatgcgcg ctctcgctgc tgagcagtcg gaagtgtcgc
     1441 cagaacagcg gcgcgccatc tgcgaggctt ttgagtctgc caataatacc catggcttaa
     1501 acctgactgc ccataaatac ccgggcttac gcggcacact acaaaccgca tccactgact
     1561 gcgacacaat tgtggaagct gcagcactat tacccgcttt tgatcaggca gtggaaggca
     1621 accgccacca ggatgattac ggttcaggtc ttgggatggc cgaagagaaa tttcactatt
     1681 atctcgatct caatgaccgc tatgtctatt tttatgagcc ggttaatgtt gaatactttg
     1741 cgatgaataa ctggtccttc ctgcagtcag gaagtattgg tatcgatcgc aaagatattg
     1801 aaaaggtatt taccgggcgt accgtattgt cgagcattta ccaggatcag cgtactaaac
     1861 agaacgtgat gagtttgctg acgccggtgt atgttgcagg gcagctaaaa gggattgtgc
     1921 tgctggatat taacaaaaac aatttgcgga atatctttta cactcatgac cgccctctcc
     1981 tctggcgttt tctcaatgtc acgctaaccg ataccgattc ggggcgcgac attatcatca
     2041 accagagcga agataatctg ttccagtatg tcagttacgt ccatgactta ccgggcggca
     2101 ttcgtgtctc gttatccatt gatattcttt actttatcac gtcttcgtgg aaaagcgttc
     2161 tgttctggat tttgacggcg ttaattttgc tgaatatggt ccggatgcac ttccgtttat
     2221 accaaaatgt gtcgcgagaa aatattagtg atgcgatgac tggactgtat aatcgcaaaa
     2281 ttttaacccc tgaactggag cagcggttgc agaaactggt gcaatccggt tcttcggtga
     2341 tgtttattgc aattgacatg gacaagttaa agcaaataaa tgacaccctc ggtcatcagg
     2401 agggggattt agcgattacg ttattagctc aggcgattaa acaatcgatt cgtaaaagtg
     2461 attatgccat ccgactcggt ggcgatgaat tctgcatcat tcttgtcgat tcgacgccgc
     2521 aaattgcagc acaactgcct gaacgtatcg aaaaacgtct gcaacatatc gcgccgcaga
     2581 aagagatcgg cttctcttcc ggtatttacg cgatgaaaga aaacgatacg ttacatgatg
     2641 cgtataaagc ttccgatgag cgtttatatg tcaataagca gaacaaaaac agccgttcat
     2701 gataaccttc tgtggttgtt tgcttgtaat ctcaggagcg tgaaatgact gaaatggcta
     2761 aaggaagcgt gacgcatcag cggttaatcg ctttattatc acaagaaggt gctaacttcc
     2821 gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt gtcagaaatt cgtggcaccg
     2881 ctttaggcca gggagctaag gcactggtat gtaaagtcaa aggcaatggc gtaaatcaac
     2941 atgttctggc gatcctcgct gccgatcaac aggccgatct gagccaactc gccagccata
     3001 taggcggatt acgcgcttcg ttggccagcc cggcggaagt cgatgaactc acaggctgtg
     3061 tcttcggcgc gatcccccct ttcagcttcc atccaaaact caaactggtt gccgaccctc
     3121 tactcttcga acgatttgat gaaatcgcct tcaatgcagg gatgctggat aaatccgtta
     3181 ttttgaaaac cacggattat ttgcgcattg cgcaaccaga actgctcaat ttccgccgca
     3241 ctgcgtaact agcctgtccg ttcaataagc agaacggaca aaaccagcac aacagcaatg
     3301 gcaaaaaacg atgacgtgat aatcagtgtc tcgacgaaca tttgatcgtt catagcatgc
     3361 ccccgggaga tagctgtttt cacgttattg ttagcgtgca caaatggcag tttgatgaca
     3421 gttcgcctga ttttttatgc aaaaaacgta aagattttct acttccttcc tgcagcaagc
     3481 gtaaagtaag caggcttatt attttttggc aaggaaacca cgatgtttga tgtcactttg
     3541 ctgatcctgc tcgggttagc tgcgctgggc tttatcagtc ataacaccac tgtcgccgtt
     3601 tcaattctgg tgttgatcat tgtccgcgtc acaccgttaa gcaccttttt cccgtggatt
     3661 gaaaaacagg ggctaagtat cgggataatc attctgacga ttggcgttat ggcccccatc
     3721 gccagcggga cgctaccgcc ctcaaccttg attcactcgt ttctgaactg gaaatcgctg
     3781 gtggcaattg cagtaggcgt tattgtctcc tggctgggtg ggcgcggcgt gaccttaatg
     3841 ggcagccagc cgcaactggt cgccgggctg ctggtaggca ctgttttagg cgtggcgctg
     3901 tttcgcggcg taccggtcgg cccgcttatt gccgcgggtc ttgtttcgct gattgtgggg
     3961 aaacagtagt taatcccgca atatatcggc ctggcgtttg ccccaggcct ttcttaaaca
     4021 tagtaataaa ggccgttgtt gaatcatacc ccagcgtatg agcaactttc tgtacagtat
     4081 cgcctttcac taatccctga agtgccataa tcagctgtaa ctgctgacgc cactggcgaa
     4141 aactcaaccc cgtctctttt actatcagtc gcgcaagatt gcgttcactc atcgcaaaaa
     4201 agccagccca ttgccctaat gacccccact cgacgggccc tttcgccatc atctccacca
     4261 tcgtgcggat tttaggatga gaagaaacgg gcaggtgcag ctgttgttgt ggctgctgag
     4321 ggagttcatc aaacaggacc tgaattaatc tgcgggtcat cggctcagca cgctgagtcg
     4381 tcgtcctgtt tgccagggtt aaaatcaatt cccggcataa gggcgatatt ttcaatgtgc
     4441 aacatgttgt cggcattgtc actgcgctgg gttcgataaa cagaaaacag agttcagcgt
     4501 tagctgtcac ttgattgcta tgttccaccc cgcccggtat ccaaaccgca tattgcggcg
     4561 gcaccatcca caaagcattt tccaccgtac aggtaattgc accatgtaat gctagtatca
     4621 gctgtccttt gcggtgctga tgtacaggac tggttaactc atccgttccg gcgtgaatac
     4681 aaaatgcgac cgcggcttca tggtgtcggt cgggttcata gccattgaga ttcaacctgt
     4741 gcatcatttt gtccgaattt agcgataatt tgtcatttta gcttgattca acataacaat
     4801 aaaaacggta aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc
     4861 attaagcggc aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt
     4921 acgcgtcacc tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct
     4981 gacgacagcg caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc
     5041 acctttggct gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt
     5101 acttttgatc tgtgcaggta tcgcaattcg ttctctccct tcgccttact tattatttgg
     5161 cggtacagcg gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat
     5221 taaacgcgat ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg
     5281 agctgcagcg gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg
     5341 gcaaggcgcg ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc
     5401 acagtggcga agccaacaac atgcaaattt gagtacctcg cgcgccttac atactcgggg
     5461 tatctggcgt tcgccgcttg cctggcaggt cacattgttt cttgggatca actcactggt
     5521 ctattacgtg attattggct ggcttccggc gatcctcatc agtcgcggct atagcgaagc
     5581 acaggcgggt tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat
     5641 ccctcttttc ttacatcatg tgaaagatca gcgtggtatt gcagcgttcg ttgccttgat
     5701 gtgcgcagtg ggcgctgttg ggctctgctt tatgccagcg cacgcgatca cctggactct
     5761 gcttttcggt tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg
     5821 ggcaagttct gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt
     5881 gctggcagcc tgtgggccgc cgctgatggg taaaatacac gatgctaacg gtaactggtc
     5941 cgtaccactg atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc
     6001 tgggagagac aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcatgcgt
     6061 gaaagggaga acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca
     6121 gagcgatggt tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga
     6181 tttagccctt gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt
     6241 tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa tatcttgcag aactggcgca
     6301 acacgagcaa gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct
     6361 ctacgcagca aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag
     6421 cttgtgattt tagtacagca tccggcggtt atttttcacc agccggatgg tcccgtcgcc
     6481 acaatgccca ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt
     6541 gcgctgtcga cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat
     6601 ttgccatacc aatctgcggc ggtttaggtg cttctggctg agaagaggtt gaacaaccag
     6661 ccaggaccag caagcaaggc aaagcaaaac tgataatttt catttattga tctcacatat
     6721 ttatccagac ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt
     6781 cactcaaaac atattacgtc ttgtttcatc tttgttggtg atgttttatc atgcctgcaa
     6841 agattaaata atcagcattt acccgccgta tcctggagtt gtttcgtgtc agatcagatt
     6901 atcgcccgcg tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag
     6961 cttctggaga tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat
     7021 gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg
     7081 gagaatttta ccgtctcgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc
     7141 tttttcagcg atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc
     7201 atcaccacat ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt
     7261 tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta
     7321 ttcgctggac tgattgcaca atatattcaa aaagaggcgc tggttgaaca gctgcgcgaa
     7381 gccaatgctg cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg
     7441 cgggcgattt ttgaaaatct gacgacgctg ttttctctcg cccggcatct taaccataag
     7501 ataatgatcg cgtttatcga tctggataac ttcaaattaa tcaatgaccg ttttggtcat
     7561 aatagtggcg atctgtttct cattcaggtt ggcgagcacc ttaatacgct ccagcaaaat
     7621 ggcgaagtta ttggtcgtct cggcggtgat gagtttttgg ttgtttcact aaacaacgag
     7681 aatgcggata tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac
     7741 ttaggtgatg ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct
     7801 gaaacaaccg atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa
     7861 aaacacaaac agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc
     7921 acatcctttt gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttta
     7981 ttacagcggt cgtattttta gcatggtttt ttattggcgg ctatgctgcc ccgggagcat
     8041 aaagatgaaa aaaacaacga ttattatgat gggtgtggcg attattgtcg ttctcggcac
     8101 tgagctggga tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc
     8161 tttgtgcaat ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca
     8221 ccaataacgg caaccacgaa gctgccaaaa ttgaagccat cgactttacc aaagccaaac
     8281 agtgtgctga tccatccgcc gactacggca ccgactatcc ccagcagaat agtcataaag
     8341 aatccaccac catctttacc tggcatgatc cacttcgcca gaataccggc aataagccca
     8401 aaaataatcc atgacagaat gcccattgtt tcctcactta tctgttttgc attagcgggt
     8461 tagtcgctga taaaaagcat agcacaacat cgggagggca agatttgtga cgagcatcac
     8521 ggaggatttt tttgcgatgg cgcagaaatt gcgccatcaa cgatcggtga taattaccaa
     8581 ccacaaacat catgttcgtt ttccgtgtct tgacatcctc cacgccctga atgacgagga
     8641 tccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct tcaacgagcg
     8701 gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg
     8761 agcgccgttt acatcggcgt tcgctgtata tccacatacc tggcatctga attgactttg
     8821 tgacaggcga ttttctttcg ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc
     8881 tggcggaaca gcaagcacct gaccgccac
//
LOCUS       DAZFRN010000180         8813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFRN010000180 DAZFRN010000000
VERSION     DAZFRN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.182"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            105..2168
                     /locus_tag="ACHKUB_004330"
     CDS             105..2168
                     /locus_tag="ACHKUB_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO6657267.1"
                     /translation="MSNILWGDLETYCEIPITNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIAAPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDVELARAAIDAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNILRDVIHEAKSWYDESVKRKSTYGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEKGNITYMGVNSYSRKWQRLKTYGGKLVENITQAAARDILAGN
                     MPLIEDAGYSIVLTVHDEVITEAPDSPDYSAEHLSQLLATVPDWATNIPLNAGGFESY
                     RYRKD"
     gene            2408..3175
                     /locus_tag="ACHKUB_004331"
     CDS             2408..3175
                     /locus_tag="ACHKUB_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO6657268.1"
                     /translation="MTSQIIPVFNGTIANETTLLCNARDLHAFLGVGKMFAHWIKDRL
                     GEYGFIENQDFVIACQNWQAKGRGGHNRKDYHLTLDTAKELAMVERNEKGRQIRRYFI
                     ECERKLHQQSSTILAPHRECLPKMVYHHRSKYNPYRAYAWDGEKSVYVGCYPTVDEAV
                     AAQERFYRNGSTKRIQKAQTAVSDAEKEMFINNLRAVCHNFRRINEIWRAQLMPALEK
                     MDSKLVYQLHDRFSDSMCILPTIEDRIGRYIPPTLPR"
     gene            3228..3443
                     /locus_tag="ACHKUB_004332"
     CDS             3228..3443
                     /locus_tag="ACHKUB_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO6657269.1"
                     /translation="MSFKYRDSPLYYRAAREALRLEQSGEYDRAAKVWAKANRQSRNE
                     LNQDWSERRSDFCLMQNMREKRKAVEE"
     gene            3443..3682
                     /locus_tag="ACHKUB_004333"
     CDS             3443..3682
                     /locus_tag="ACHKUB_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657270.1"
                     /translation="MRNTAKLQLGFSPLSKKIMLAKMRDVDGGRLRVGNDPGRDVTNE
                     AAQLVWQLVMAEGGEISWGLDDGSQMVLRAEKQVK"
     gene            3683..3976
                     /locus_tag="ACHKUB_004334"
     CDS             3683..3976
                     /locus_tag="ACHKUB_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO6657271.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIVFVECKAPSKPPRPDQLREHERLRALGFTVVVLDSKNLEGILCGEPITTK"
     gene            3949..4977
                     /locus_tag="ACHKUB_004335"
     CDS             3949..4977
                     /locus_tag="ACHKUB_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO6657272.1"
                     /translation="MRGAYYNEIDPNAAQWLRNLIAAGHIAPGEVDERSIEDVAPDDL
                     RGFTQCHFFAGIGVWSYALRLAGWPDNKPVWTGSCPCQPFSSAGKGAGFDDERHLWPA
                     LFHLISERRPKLVFGEQVAGVNAWFDLVQTDVEAVDYAFGLVPFPAAGVGAPHIRDRA
                     YWVADASGERSQRWRKRLNSIEEDVWRSSTVRQHATQLPAEFCSPSGVVHPNLQHKPT
                     AGDKTRSRNLSGTIQISGMADSDDDRQQPSSCRGRSGQSYRTGNDPRRRCENLRPNTI
                     NGCWDGADWLLCRDGRWRPVESGTCPLANGITSRVGRLRAYGNAIVAPAAATFIRSFM
                     ECAGYDLI"
     gene            complement(4974..5294)
                     /locus_tag="ACHKUB_004336"
     CDS             complement(4974..5294)
                     /locus_tag="ACHKUB_004336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657273.1"
                     /translation="MATLLTSGLTVPEYYKNGGVLDFELDALEVGGNSTDFENYPSLV
                     NILSKGFELPATSMVSDPKFLAPILVYGDFWTKLHAYTYAMGGSVVYKQLPSGRYHAR
                     CEWH"
     gene            5329..6723
                     /locus_tag="ACHKUB_004337"
     CDS             5329..6723
                     /locus_tag="ACHKUB_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFO6657274.1"
                     /translation="MPKVFTPRPYQDLIINHEIDIHRCNIWAGMGMGKTVATLTTLED
                     LFMAGSETQPALVLAPLRVAASTWPDEALKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVQ
                     RWINLTGTPAPNGLVDLWGQLWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSHAEIQRALADVTLSLDAADWFDIKDLIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            complement(6917..8314)
                     /locus_tag="ACHKUB_004338"
     CDS             complement(6917..8314)
                     /locus_tag="ACHKUB_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6657275.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADCGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            8529..8789
                     /gene="yfhL"
                     /locus_tag="ACHKUB_004339"
     CDS             8529..8789
                     /gene="yfhL"
                     /locus_tag="ACHKUB_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO6657276.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT         2281 a         2276 c         2313 g         1943 t
ORIGIN      
        1 atccagctat tttctccaga tgctctgtat ggtcaatcaa gtattggatc atatgcataa
       61 ttaatctttt aatcagttaa tagaataaaa accaaggtaa acacatgtcc aatatactat
      121 ggggcgacct ggaaacctat tgcgaaatcc ctattacgaa cggtacccat gcgtatgccg
      181 aaggcgttga agtgatgctt ttcgcatggg ctatcaacga cgggccagta aacgtgtggg
      241 atatcactgc cggtggtggt attccacacg gcttatacga agcaatcgca gcccctgaaa
      301 ccctgcttta tttccataac tctcacttcg accgcaccgt tctgcgttat gcaatgccgc
      361 gcctggcacc gccagtcgaa cgttggcgcg acacaatggt gcaggcgctg gcgcacggtc
      421 tcccggggtc tctgggggaa ctctgcgaag tactaggcgt cccgcaagac aaagcgaagg
      481 acaaagaagg taaagcgctg atccagctgt tctgtaagcc acgcccgaaa aacagcaaac
      541 tgcgccgggc caccagcaaa acccacccgg aagaatggcg gcgctttgtt gcttacgccg
      601 gactggatat cgaggcaatg cgcgaagtct ataaacgtct gccgaagtgg aattatcagg
      661 ggacagaact ggcgctctgg catcgtgatc agcagatcaa tgaccggggc gtctgcatgg
      721 acgtggaact cgcacgcgct gcgatcgacg cggtagacca ggaacaaaag cgcctggcaa
      781 agcgtacaca ggaaatgact gatggcgaag tgcaggcagc cacacaacga gacgcgttga
      841 ttaagcacat tgttgaatcc tacggtgtgg agctaccaga catgcaacgc agtactctgg
      901 aacgtcgtat tgccgacccc gatttaccat ctgccgtgaa agaactgctg gctatccgcc
      961 tgcaggccag tactaccagc accagtaaat acaaggcact gatgaaaggc gtaagccacg
     1021 acgggcgctt acgcggtacg ctacagttct gcggggcgtc acgtaccggt cgttgggccg
     1081 gacggctatt ccagccccag aaccttcccc gcccttcact aaaacaggaa caaatagacg
     1141 aaggcatcga agcactgaaa gccggatgtg cagacctgct gtttgacaat atcatggaac
     1201 taaccagttc agcgttacgt ggctgcatta tcgcgccaac aggcaaaaag ctggtggtaa
     1261 gtgacttgtc gaacattgaa ggccgtatgc tggcatggct ggcgggagaa gaatggaaac
     1321 tgaatgcatt cagagagtac gacgccggaa cgggtccgga cttatataaa ctggcgtatg
     1381 caaaagcttt cgatattgca ccagatgatg ttgataaaca catgcgtcag atcggtaaag
     1441 tcatggaact cggtctgggt tatggtggtg gtgtgtcggc cttcatcact tttgcactgg
     1501 tttacggtct cgatctcgac gagctggcga acgccgcact accaaacatt ctccgcgatg
     1561 ttatccacga ggcgaaaagc tggtacgacg aatcggttaa acgcaagtca acctacgggc
     1621 tttctgaacg ggtatttatc gcctgcgact cacttaaacg tctctggcgc cgggcgcatc
     1681 ccgcgacctg tgatttctgg tacgaactgg agcgcaccgt ccgcactgca atcgccacac
     1741 cgcaaaaaac attgtattgc ggttatctta aaatccgccg cgatggcgcg tggctgcgca
     1801 tacagctacc atcaggacgc gctgtatgct acccgtctcc ggttatcgaa aaagggaata
     1861 tcacctacat gggggttaac tcttattcgc gtaaatggca acgactcaaa acctacggcg
     1921 gaaagctggt ggagaacatc acccaggcgg ccgcccgcga cattctggcc ggaaacatgc
     1981 cgcttatcga agatgccggt tacagcattg tgctgacggt acacgacgag gtgatcaccg
     2041 aagcacctga ctcacccgat tactcagcag aacatctcag ccagttactg gcaaccgttc
     2101 ctgactgggc cacgaacatt ccactaaacg ccggaggatt cgaatcttac agataccgga
     2161 aagattgaca tccgcgatga ccaataggta tattccgctt catggtgctc aaaacacctt
     2221 gcaaagcgga aaccgcaccc gacagtcatg cggatttttt atgtccacga tattgagaat
     2281 tcaatggtcg ggcgtgcggc taatacaata cccgtaaggg aaatacgccc gccgactttg
     2341 cacggttttg agcgcctgac caccctctca aaaggggtga attcaaaata ttgcaaagga
     2401 cataaaaatg actagtcaaa tcatccccgt attcaacggc actattgcca acgaaaccac
     2461 cctactctgt aatgcccgtg atctgcacgc ttttcttggt gtgggtaaaa tgtttgctca
     2521 ctggatcaag gacaggctgg gtgaatatgg attcattgaa aatcaagatt ttgtgattgc
     2581 ttgccaaaat tggcaagcaa aaggtagagg cggtcacaac cgcaaggact accacctcac
     2641 cctcgacacc gctaaagaac tggcaatggt cgaacgtaac gaaaaaggcc gccagatccg
     2701 ccgatacttc atcgagtgcg aacgcaaact gcaccagcaa tcctccacga tactggcgcc
     2761 acacagggag tgtctgccga agatggttta ccatcaccgg agcaaataca acccgtacag
     2821 agcttacgca tgggacggag agaaaagcgt ttacgtcggg tgctatccga ctgtggatga
     2881 ggctgtcgct gctcaggaac gcttctaccg gaacggcagc acgaaacgca ttcagaaggc
     2941 gcagacagca gtcagtgacg cagagaagga gatgttcatc aacaatctcc gcgccgtctg
     3001 tcataacttc cggcgcatca atgaaatctg gcgggcgcag ttaatgcctg ctctcgaaaa
     3061 aatggattcg aaactggttt accagttgca tgaccgcttc agcgacagca tgtgcattct
     3121 gccgaccata gaagaccgca tcggcagata tatcccaccc actttacctc gttaatcctt
     3181 cagttcgccc ctgctcagcc aggggctttt tacaggagtc gaaagatatg tcatttaaat
     3241 accgggacag tccgctttat taccgggctg cgagggaggc cttgcgcctt gagcagtccg
     3301 gcgaatatga ccgggcagcg aaggtctggg ccaaggctaa ccgccagtca cgtaacgaac
     3361 tgaatcagga ctggagcgaa cgccggtctg atttttgtct gatgcagaac atgcgcgaaa
     3421 agcgtaaggc ggtcgaagaa tgatgcgtaa tacagcgaaa ctacaactgg ggttctcccc
     3481 gctatccaaa aagatcatgt tggcaaaaat gcgcgatgtt gacggcggtc gcttgcgagt
     3541 tggcaatgac cctgggcgtg acgtaacgaa cgaagccgcg cagttagttt ggcaactggt
     3601 aatggcagaa ggcggcgaaa ttagctgggg tcttgatgat ggtagccaga tggtactgag
     3661 ggctgaaaag caggtgaagt aaatggccta cgaacgtgaa agccttatcg aaaagcacct
     3721 cgtcgccgaa gtgaaaaagg ctggcggggt cgcctttaag ttcgtgtcac ccggtcgccg
     3781 ctcggtaccg gatcgcattg tcctgctacc cggcggtcgc atcgtctttg tcgaatgcaa
     3841 agcgccaagc aaaccaccac ggcctgacca gttacgcgag cacgaacggc tgcgcgcgtt
     3901 gggctttacc gtggtggtat tagatagcaa aaatctggag gggatattgt gcggggagcc
     3961 tattacaacg aaatagaccc taacgcagca caatggctgc gtaaccttat tgctgcaggt
     4021 catatcgccc cgggtgaagt tgatgaaagg agtatcgaag atgtcgcacc agacgacttg
     4081 cgcggattca cacagtgcca tttcttcgcc gggatcggcg tctggtctta tgctctgcgt
     4141 ctggccggat ggccggataa caaaccggtc tggacaggaa gttgcccgtg ccagcctttc
     4201 agctcggctg gtaaaggcgc tgggtttgat gacgagcggc acctatggcc agcactattc
     4261 cacctcatca gcgagcgacg ccctaagctg gtatttggcg aacaggttgc gggagtcaat
     4321 gcctggttcg atcttgtaca aactgacgtg gaagcagtgg actacgcctt tgggcttgtc
     4381 ccgtttccgg ctgcgggcgt cggtgccccg catatccgag atagagcgta ttgggtggcc
     4441 gacgccagcg gcgagcgatc acagcggtgg cgaaagcgcc tcaatagcat tgaggaagat
     4501 gtctggagat cgtcgaccgt ccggcaacac gctacacagc tccctgcgga attttgctca
     4561 cctagcgggg tggtacaccc caacctgcaa cacaaaccca cagccggaga caaaacgcgg
     4621 tctagaaacc ttagcgggac tatccagatt agcgggatgg ccgacagtga cgacgatcga
     4681 caacaaccaa gttcgtgccg agggcgcagc ggccaatcat accgaacggg gaacgaccct
     4741 cggcggcgct gcgagaatct gcggcccaat acgattaacg gctgctggga tggtgctgac
     4801 tggctcctct gccgggatgg tcgctggcgg ccagttgaat ccggcacatg cccgttggct
     4861 aatgggatta cctccagagt gggacgactg cgcgcctacg gcaacgcgat agtcgcacca
     4921 gctgccgcaa cattcatccg ctcgtttatg gaatgcgcag gatacgacct tatttaatgc
     4981 cactcacatc tggcatgata ccgtccagaa ggtaattgct tatatacaac ggagccgccc
     5041 atggcataag tataagcatg aagttttgtc cagaagtccc cataaactag tattggggct
     5101 aaaaattttg gatcgctaac catagatgta gcaggaagct cgaaaccttt agaaagaatg
     5161 tttaccagtg aagggtaatt ttcaaaatca gtgctattcc cgcccacctc taaagcatcc
     5221 agttcaaagt cgagcactcc accatttttg taatactccg gaacagttaa ccctgatgtt
     5281 aacaaagtag ccattaaaca atcctcctga attttaaagg gttacattat gccaaaagtt
     5341 ttcacgcctc gcccctacca agatctaatt atcaaccacg aaatcgacat tcaccgctgc
     5401 aacatctggg cagggatggg gatgggtaaa accgtagcga cactcaccac gctggaagat
     5461 cttttcatgg cgggctcaga gacacagccc gcgctggtcc tcgcgcccct acgcgttgca
     5521 gcaagcacat ggccggatga agcgctgaaa tgggggcatc tgcgcaatat cgaggtgcag
     5581 ccgattgttg gtaacgccaa agcacgctct gcagcgctgg cgaacagcaa cgcgagcgtg
     5641 ttcaccatca actacgataa ccttgtctgg ctggttgagg aattgggaga acgatggccg
     5701 ttcggtactg tcattccaga tgaaagcacc cggctaaaat ccttccggct gcgaggtggt
     5761 ggtaagcgcg cggcggcgct gggcaaagtg gcgcataagt atgtccagcg ctggataaat
     5821 ctcaccggta cgccagcacc gaacggcctg gtagatttgt ggggacaatt gtggtttgtg
     5881 gaccaggggc aacgtctcgg gcgcacttac ggcgcgttta cctcacgctg gttcaactcg
     5941 atacagtttc cggggcagag ctggaccaaa ctggagccgt ttgctcactc acatgctgaa
     6001 atacagcgag cgttagccga tgtgaccctc tcgctggatg cggccgactg gttcgatatc
     6061 aaagacctca tccataacgt aatccgcgtg gatatgccgc cgaaggcccg tcagcagtat
     6121 cgtgaaatgg aaaaggaaat gttcctcgag ctgaacggcg aaggcatcga agcaccaaac
     6181 gccgcggcaa agacactgaa gtgtctgcaa atcgccagtg gcgcagtata cacagacgac
     6241 accggaagtt ggtcagaact gcatgacacc aaactacaag cgctggacag catactgacc
     6301 gaagcagctg gcgcacctgt gctggttgct tatcactgga aacacgatct tgaacgcttg
     6361 cttaaagcat tccctcgcgg tcgtcacctc gaccaagatc cacagacact gcgcgactgg
     6421 aattccggaa agattcctgt tctctttgca cacccagcca gcgcgggcca cggtctgaac
     6481 atgcaggacg gcggaaacat actggtattt ttctcacact ggtgggacct ggagcagtat
     6541 cagcaaatta ttgaacgtat cggccccacc aggcagatac aggccggaca caatcgtccg
     6601 gtatttattc accacattat tgcggccgac actatggacg aaatggtgat ggagcggcgc
     6661 aactcaaaac gaacagtgca ggacatcctg ctcgatgcca tgaaaaagag aggtatagca
     6721 tgagcgagaa acccgacgat ttactcaccc cagatgaagt atgccaaaag ttaggtatta
     6781 cacagaaaac gctatgtgag tggaatatta agcatcgtca tcgggctatc ctggcaccaa
     6841 ttcgtttcag tgcaaaagta gttcgttatg agcgccgaaa tgtcgacgct tttattcaaa
     6901 agtgtcgcag ccagtattaa cctcgccgcc gtagcaatgc aacctgcgca agtatgctcc
     6961 gctcgtgagc ctcgaaagct tcgcgcttta acgcaatctc ttcctgtaaa atctcatcag
     7021 aaaagtcgta atgttctgcc atcgggtcat ctgacttgct ggagtggtga aggcaaagga
     7081 ggctgatttc ccttcggtct gatcgggaat agcctctttc cttcatcaag gcaataacat
     7141 tgctcttaag gaatttacgg cacatcgtat taaatgcacc gtctttccct ttaacagtcc
     7201 catcatgttt tattcctttt acagccccgt ccgggctgta tgttttcacc agcttatcca
     7261 gtgatcgttt tgaaaatggc tgcattggat cacgtggctg caaaaataca taatccctgt
     7321 tgcactcagg aactgaatca cgccaggctt tctgctcgtc gataatccgc cggatctcag
     7381 gggttatcgg caggcggaaa gccttttgtg ttttcatagc ccctcgcatg ccgataaccc
     7441 cttccggata aacaatttca ccagcctcct cgtgaacgta gtcccagcgc aggttatgga
     7501 cattaatcgg acgaacgcca gtgatgatca tgaagcgaac agcattcttc tggtgtacag
     7561 aggtgcaggc agcaacattg agccagagtc gggcgattga ttcaatatcg gtaaaaagcc
     7621 gtgtgggggt aggtttctgt acgcgggagg aaacataatc atctggcaga ctggcggcaa
     7681 cattgcggcc gttgcaaaga gtaggtgcgc agaacttcca gaaccgacgg agctcggcaa
     7741 acaactccag ggcgttattg ttcgagcgag tggcgatcca ctcgtccagc acttccacca
     7801 gccgattgta tgttacgtcg ctgaacacct cacgctcgcc gaacgttgct ttaatccggt
     7861 cgatacgcac cccgtaggtt gtgaaactgt ccgggctcag cttctgccgg gcgactttgg
     7921 ctttgaggtc atcccggtac atttccagcg ctgcatgtac tgactctgcc cgcaagccac
     7981 agtcagccat acctagcgct ttttcgcgcg ccagctggat agcgagctcc ggccactcgc
     8041 cgagcttttt acccttgagg cccatctttt ttggaaactc ggcgtaaaat gtaaccttac
     8101 cggctttgct gaaatcgata cggagatagt tctctttttc gtatttggaa cggcgagcca
     8161 cgccggaagc agcgaggatg attttggcag cagcaacaca gattttcatg tgtgcgctgg
     8221 tatagggggg tttacaggcg tcccattttt cagatgcggc taaaacatcg tcattattgg
     8281 ggctatccgg attatgtgtt acagtgcgcg gcattctcaa tccttatctg cgtaggcgca
     8341 gaaaacaagc tcacacatac aagtcttttc tacgggacaa aatgcaatgt gttgcggttt
     8401 tgtgttgctg gactgagttt atcaaggtta aatgcactgg atcaacatac agtaagttaa
     8461 tgacagtaaa gcatacaaac tcgatacaac ttactgattt taaaatgatt taacggtaat
     8521 tcattgaaat gtctttacta attactaaac gctgtattaa ttgtgatatg tgtgaacccg
     8581 aatgcccgaa tgaggcgatt tcaatgggag atcatatcta cgagattaac agcgataagt
     8641 gtaccgaatg cgtagggcac tacgagacac caacctgcca gaaggtgtgc ccgatcccca
     8701 atactattgt gaaagatccg gcgcatgtcg agacagaaga acagttgtgg gataaatttg
     8761 tgctgatgca ccacgcggat aaaatttaat attctactct ggaagtagag tat
//
LOCUS       DAZFRN010000181         8766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFRN010000181 DAZFRN010000000
VERSION     DAZFRN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.183"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            104..1153
                     /gene="allD"
                     /locus_tag="ACHKUB_004340"
     CDS             104..1153
                     /gene="allD"
                     /locus_tag="ACHKUB_004340"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO6657277.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1175..2410
                     /gene="allC"
                     /locus_tag="ACHKUB_004341"
     CDS             1175..2410
                     /gene="allC"
                     /locus_tag="ACHKUB_004341"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO6657278.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2421..3206
                     /gene="allE"
                     /locus_tag="ACHKUB_004342"
     CDS             2421..3206
                     /gene="allE"
                     /locus_tag="ACHKUB_004342"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO6657279.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPRGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3335..4480)
                     /gene="glxK"
                     /locus_tag="ACHKUB_004343"
     CDS             complement(3335..4480)
                     /gene="glxK"
                     /locus_tag="ACHKUB_004343"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO6657280.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEIEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4502..5802)
                     /locus_tag="ACHKUB_004344"
                     /pseudo
     CDS             complement(4502..5802)
                     /locus_tag="ACHKUB_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(5859..7220)
                     /gene="allB"
                     /locus_tag="ACHKUB_004345"
     CDS             complement(5859..7220)
                     /gene="allB"
                     /locus_tag="ACHKUB_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO6657281.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7280..8734)
                     /locus_tag="ACHKUB_004346"
     CDS             complement(7280..8734)
                     /locus_tag="ACHKUB_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO6657282.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNANGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT         2344 a         2300 c         2113 g         2009 t
ORIGIN      
        1 attctaacta tatttttttg cctgtctgga tcacataatc cagatatttt ccctgtatgt
       61 taattgcagt catgcttcac accgtcgtta aaaaggaaga cagatgaaaa tcagtcggga
      121 aacactccac cagctaattg agaataaact ctgccaggct gggttaaaac gtgagcacgc
      181 tgcaaccgtg gctgaagtat tggtttacgc cgatgccaga gggatccact ctcatggcgc
      241 ggtgcgcgtg gaatactacg cggaacgcat ttcaaaaggc ggcaccaacc gcgaaccgga
      301 gtttcgtctt gaggaaaccg ggccgtgctc ggcaatttta catgccgaca atgccgccgg
      361 acaggtcgcg gcgaaaatgg gtatggaaca tgccatcaaa accgcccagc aaaatggcgt
      421 tgcggtggtc ggtatcagcc ggatgggtca cagcggcgca atctcttatt ttgtgcagca
      481 ggcagcccgc gccggattaa ttggtatttc gatgtgccag tccgatccaa tggtggtgcc
      541 gtttggcggc gcggaaattt actacggtac taacccactg gcctttgccg cgccgggaga
      601 aggcgacgag atccttacct ttgatatggc gactaccgta caggcatggg gaaaagtgct
      661 cgacgcccgc tcgcgtaata tgtctatccc ggatacctgg gcggtcgata aaaacggtgc
      721 accaacaacc gatccgttcg cggtacatgc tctgctcccc gccgccgggc caaaagggta
      781 tggcctgatg atgatgattg acgtcctctc aggcgtctta ctcggcttac cgttcgggcg
      841 acaggttagt tcgatgtatg acgatttaca cgcagggcgt aatttggggc aattacatat
      901 agttattaat ccgaactttt tctcctccag cgaattattc cgtcaacatc ttagccagac
      961 catgcgcgaa ttaaatgcca ttacccccgc gcccggtttt aaccaagttt attatcccgg
     1021 acaggatcag gatattaaac aacgcaaagc cgccgtcgaa ggcatcgaaa ttgttgatga
     1081 tatttaccag tatttaattt ccgacgcgct ttataacacg tcatacgaaa cgaaaaatcc
     1141 ctttgcgcaa taattattga gacaggactt tcttatgatt acacattttc gtcaagccat
     1201 agaagaaacg ctgccctggc tttcctcttt tggcgctgac ccaacgggtg ggatgacccg
     1261 tttactttat tcgccggaat ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc
     1321 aagtgggctg gaaacacgtt tcgatgaagt agggaattta tacggtcgcc tgaatggtac
     1381 cgaatatcca caggaagtga ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg
     1441 taacctcgac gggcaattcg gcgcgctggc ggcgtggctg gcaattgact ggctgaaaac
     1501 gcaatacggc gcgccgctac gtacggtcga agtggtggcg atggcagaag aagaaggcag
     1561 ccgcttcccg tatgtcttct ggggcagtaa aaatatcttt gggctggcga atcctgacga
     1621 cgtgcggaat atctgtgatg ccaaaggaaa tagttttgtc gatgcgatga aggcttgcgg
     1681 atttactctt ccgaacgccc cactaactcc gcgtcaggat attaaagcct ttgttgaact
     1741 gcatattgaa cagggctgtg tgctggaaag taatgggcaa tcaattggcg tggtgaatgc
     1801 aattgtcggg cagcgtcgtt atacggtaac gctgaacggc gaatcaaacc atgcaggcac
     1861 cacgccgatg ggttatcgtc gtgatacagt ttacgctttc agtcgcattt gccatcagtc
     1921 ggtcgaaaaa gcgaaaagga tgggcgatcc gctggttctg acctttggca aagtagagcc
     1981 gcgcccgaat acggtaaatg tggtgccggg taaaaccacg ttcaccattg attgtcgtca
     2041 taccgacgct gccgtgctgc gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat
     2101 ttgcgatgaa atggacattg gtattgatat cgatttatgg atggacgaag aacccgtgcc
     2161 gatgaataag gagctggtcg ccaccctgac agaattgtgt gaaagtgaaa aactgaatta
     2221 ccgggtgatg cacagtggtg ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac
     2281 ctgcatgatt ttcattccca gcatcaacgg gatcagccat aacccggcgg aacgcaccaa
     2341 tattaccgac cttgccgaag gggtcaaaac gttggcactc atgctttatc aacttgcctg
     2401 gcagaaataa ggagtcataa atgggatatt taaataacgt caccggttac cgcgatgatt
     2461 tactggctaa ccgtgcgatt gttaaacacg gtaatttcgc actgttaacc ccggatggtc
     2521 tggtaaaaaa tattattccg ggctttgaaa attgtgacgc gacaatcctc tccacgccaa
     2581 agctgggtgc ctcttttgtt gattatctgg tcacactgca tcaaaacggt ggcaaccaac
     2641 agggcttcgg tggcgaaggc attgaaacgt tcctgtatgt gatctctgga aatatcactg
     2701 ccaaagccga aggcaaaaca tttgccttaa gcgaaggtgg ctatctttat tgcccacgag
     2761 gctccttaat gacgtttgtt aacgcccagg ccgaagacag ccaaatcttt ttatataagc
     2821 gccgctatgt tccggtagaa ggccatgcac cgtggctggt ttctggcaat gccagcgaac
     2881 tggaacgcat tcactatgaa ggtatggatg atgtgatcct gctggatttt ctgcccaaag
     2941 agttaggttt tgatatgaac atgcatatcc tctcttttgc accaggtgcc agccacggtt
     3001 atatcgaaac acatgttcag gaacacggtg cctatattct ttccggtcag ggggtttata
     3061 acctcgacaa taactggatc ccggtgaaaa aaggcgatta catctttatg ggcgcttatt
     3121 ctttacaggc tggttatggc gtagggcgtg gtgaagcgtt cagctatatt tactcgaaag
     3181 attgtaaccg cgacgtcgag atttaagtca taccactcgc ctgattattt ttagctatgt
     3241 tggattttgc cacggtttat accgtggctt tttttgtagt aaattcaggt aaaaaaagat
     3301 gcgatataaa tcgcatccgc tttaaaggta agggtcagtt tttaattccc tgacctattt
     3361 taatggcgca ggcaatattt cgtgcgctgt tgaagagatt ggtttcaccg ctggcgagca
     3421 cttcggctaa aggtgccaga cgtggcaaaa tgctgaatac cgcatcaatg ccgtactggt
     3481 gcaccacttc cacgccatca cccaatacgc cagcaatccc aatcaccggg acattaaact
     3541 gcttcgccac cgacgccaca cccagcggcg ctttaccgcc tgccgtttgc gagtcgatgc
     3601 gcccttcccc ggtaatcacc agtgctgcgc cctgcactgc ctgcgcaaga ttgaccgcat
     3661 tcaacacaat ctcaatacct ggtttaatat ccgcattgag aaataccgcc gccgcaatac
     3721 ccataccgcc cgcagcgccg ccgcccgcca tctggcagac gttgatttca gtttgctgtt
     3781 gtaaaacacg ggcgtaattt tgcagcccct gttccagctc ttcgaccatc tccggcgttg
     3841 cccctttttg cgggccaaat accgccgccg cgccgcgtgc ccctaccagc gggttatcaa
     3901 cgtcacatgc cacttcaata tggctgttcg ccagacgcgg atcgcattcc tctatctcaa
     3961 tgcttgccac gcgcgctaaa ttgccgccgt tagccgccag cacctggccg tcggcatcaa
     4021 ggaaacgcac accaagcgcc tgcgccatgc ccataccgcc gtcgaccgtc gcactgccgc
     4081 caatgccgag aataatatgg cgaatgccgt tatccagcgc atgacgaatt aactcccccg
     4141 taccaaaact ggaggccagc aacggattac gcttttcagg cgcgaccagc atcaggccac
     4201 ttgctgccgc catctcaatc accgccgttt tcccgtcgcc ggtaaggcca taaaaagcat
     4261 tcactttttc gcccatcggc ccgcagactt caagcgtcac gatgttgccg cccgtcgcgg
     4321 cgaccatcgc atccaccgtt ccttcgccgc catccgctat cggcaaacag atatagttcg
     4381 catcgggaaa gagggtcgaa aacccggctt taattgcctg acaacatttt tctgcactta
     4441 agctctcttt aaaagagtct ggcgcaatga caatcttcat tatcaatcct ttttcaacaa
     4501 attattcgat acgttcccac ggaataaggt tatccataag aacagccagt aaaatcccga
     4561 ccaataagcc gttactgagc agaggacgaa gcgttaatgg aaggtcttgc agatacacag
     4621 gcggtaatgc cattaaaaat atgccgacaa ataacggcaa tgcgagtcga taaatattgc
     4681 gagcggtaaa ggttatttgc tggctaaaca ccagcgcgga aaagagtaaa ggcagataag
     4741 aaaccagcat gaccgcgcta ctcacgggta aagggatact gcaaaacagt cgtgtgagtg
     4801 ccggaaccag cgccaccagc aggcaaataa cgctgccata aataaacgaa cgccgcgtgt
     4861 aatcgcctgt ctgggttaat aaaccaatgg atgaaacaaa cggtgaaaat ggaattaccg
     4921 ccagcggtac ggttatcagc gtcataaatc cggtcgccac aaagctgcga cgataacgcg
     4981 tatttcccgc gccctgctgc ggataaaaaa catccgtgcc ccgaatcgca ccgtaggtat
     5041 tgctgatatt caccagacct gtaatcaccg ccgtcagaat aattcccgga gacaaagcac
     5101 ctccactgcc gagcgggaac cactgccagt gcaactcacc ggagagcgag tgcgaagaag
     5161 gaaagcaaaa gtaccacaac aaccagccgg ttatggtgcc gaccagcagg ccataacggg
     5221 caaaacgttg cggcaggaag ataatcatcc ccagtaccag gcacatcacc gccaccgaga
     5281 gcgcgaacgg cggtaactga attttaaaat ttgggtcggc tatgccaaac ggcagccgag
     5341 catacctttg aaaaagatag tggtcagctg cgcgcccagc atcaacataa acaagaccat
     5401 caccgacggc gtaaataacc gcgccaggcg atggcctaat ccgctaaaac caatcaacac
     5461 cgtcagcacg ccggagagtg caatccccac tgccaggctg gtggcgatat cgttgatcga
     5521 tgtcccgcgc gatgcttcgc caagggtgat agtaaggatg gttccccacc acaggccacc
     5581 tggcccttcc ataatagcgc gacgatgacc gcagaacgcc tgagcgaagc aggccagtgc
     5641 ggtagcaaga aaagcatatt gcgtgagtgt aagcaggcta ctttgcggca actgaaaagc
     5701 agaaagtagc gtaggaggaa ccacaaccgt gttgcaaaaa ataaagaaaa accactgaaa
     5761 tcctgataac aggctttcgc ggctcactgc aaaattgaac ataaacacac cttaaccgga
     5821 gaatgcccgc cgcaaggacg ggcattgcag gggccagatt actgctgatg tttaaggata
     5881 aattgacctt tcggcgcaac agggaagccc tgttcaatgt cgtaaatcac atcaccacgt
     5941 aagatggttt tcgtgatacg cgcgccaatg gtacggccaa catacgggct gactttgtgg
     6001 cgatattcca gatcgtcatt ggtaagaaca tagctgctat tcggctgaat gaagacgaag
     6061 tcggcatctt ttcctggggc gatacggcct ttttgctgca gaccgaaaat atctgctgcg
     6121 ttagtcgcca ttaatttgcc gaacattggc agagacattc cgcgtttctg taccgcttca
     6181 tcgaacatca cgtccataca gttttgcaga ccggcaatac cgccccatgc ttccatgatg
     6241 ttgccggctt tcatttccgg agggcatggt gagtggtcgg aaaccaggca gtcgatttca
     6301 ccgttaaaca gtttttccca catgcctttc tggttttcca gatcgcggat cggcggtgaa
     6361 cacttcgcca gagtgccgat ttcttcgaac tgatcggtat ccagtacaaa gtaatgcggg
     6421 caggattcac aagtaacgtc ctgaccttcc tgacgtgcac gagtcacttc ttcaacgcct
     6481 tccgggctgc tgatatggca aacgtgcaga cggcaaccgg caactttcgc cagatacagc
     6541 acgcggcgaa tcgcttccac ttcggtaaat accggacgcg aagccacata gtcatgggca
     6601 gttacgcgac cttcacgttt cgcttcttcg cccagtgcgt cacagatcag cgcgttttcg
     6661 cagtgcacca gcaccggctg ccccagttcg cccagcttct gcgcaccttt gaagaactgc
     6721 cagtcgttta catcacggaa gtcgttgtcg ataccgcgat cgccacaggt cgcaacgaag
     6781 catttgaagc cgacaacgcc cacttcatcc agctcatgca gacggtcgat gttgtaagac
     6841 accaggccac cgagttgtgc cgcatcgata gtcagcttgc ctttagcggc atcgaacttc
     6901 agttcaattg aagcgcggtc aaccgttgca ggcagctggt tgagcggcat ttcgatcatg
     6961 gtggtgatac caccttttgc cgctgcgcga gtaccggttt cataaccttc ccagtggcta
     7021 cgacccggtt cagaaatatg ggtgtgcgca tcaaccatgc ccggcgaaac caccagacca
     7081 gacgcatcca taacttcttt tgcatcgccc agatcctgac cgatagcagc aattttcccg
     7141 cctttaacgg cgatatctac aacgcgagct tcgttttcta aaataacggt gccgttttta
     7201 atgattaaat caaaagacat aacaaactcc ttgtaaaata cagattggtt tttaactcaa
     7261 tgttcaatat cgggattaat taacctatgg ttttttgctc tcctgttttt tctgctgttg
     7321 tacgtttctt taataaggcg taggccgcaa aggcgacgat gacgccgaca aaccatgaaa
     7381 cacgcgataa cggttccata aagggaataa acttaccgcc aagagataaa ataacggcca
     7441 ccagagttac tgaaaacgcg gtgaggttaa aaccgttatc gtaatattta taatcgccag
     7501 gtgcggtata cagttcatca agattaattt tcccacgcat caccacaaaa taatgcgcca
     7561 tcatgacacc aattaccgga ccaagcattc cgccgataat atcgaggaac agataaatac
     7621 tgtcctgatt ttccattaat ttccacgggc agatcagcag gctgataata ctggcaatca
     7681 gtacgccgtt tttataggtc agtttcgtcg gtgcaatagc ggcaatctga taaccggcag
     7741 gaataatatt accgttggcg ttagtggaga tagtcgtcat cagaataacc agtaccgcaa
     7801 agaacgaggc gaacaagctg tcccaacgct gaacaatatc cagcacgttc caggtatcag
     7861 cgccgtaatg aatactggct ccggcaataa tacatacccc cgcgaccgca aacagaatat
     7921 aggccacaac taaacccagc gtttgcccca gcgcttgctc acgaaacgag tgggcgtttt
     7981 gcgtaaagtc ggatgcgctc accgccggtg ccgcccagac cgcaactacc gcgtttatca
     8041 ccaccaggaa caggaagcca ctgttttctg ctttctgaat accgctcgga atgtagtcaa
     8101 agattggacc gagcccgacc agcgaaatcg cccaaatcgc cataccgccg aaaacgatat
     8161 agatgcacgg gttaagaatg gcagtgaatt tatttaaaac tttgccaccg ccaaaaccta
     8221 taccaacgtt gaccagccag aagagtaaga aagtaattaa gcccggtaga gaaagcccta
     8281 acagcgtgaa atcaccaccg agagttaaaa atcccggcca gattttgcca atcagaatca
     8341 agcaggccag tgaccccgcg taacattgca ggccaaacca cataatggca gcaataccgc
     8401 ctcttaataa tccgggaaac agtgcgccac gtacgccgta agaagcacgc aggatcatgg
     8461 caaacggtac gccgtattta ctgcccgcag cgccgtttaa taccattacc gcggcaatga
     8521 aaaaggcgct gaggataatt gccagcataa tactaaaggt agacaagccg agaataaaaa
     8581 agccgccgac catcacataa ttgggaacgt tatgaaccga acccatccat aaggtaaaat
     8641 agttaaatgt tttccaggtc cgctggcttt gcgttttcgg caatagatct tcgctatagc
     8701 cgcgttgctg gaatagtttt ctctgatgtt ccataaatcc ctcttaattt cacgtatcga
     8761 ataacg
//
LOCUS       DAZFRN010000182         8642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFRN010000182 DAZFRN010000000
VERSION     DAZFRN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.184"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(296..559)
                     /gene="rpsT"
                     /locus_tag="ACHKUB_004347"
     CDS             complement(296..559)
                     /gene="rpsT"
                     /locus_tag="ACHKUB_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO6657283.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            662..880
                     /locus_tag="ACHKUB_004348"
     CDS             662..880
                     /locus_tag="ACHKUB_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO6657284.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            888..1829
                     /gene="ribF"
                     /locus_tag="ACHKUB_004349"
     CDS             888..1829
                     /gene="ribF"
                     /locus_tag="ACHKUB_004349"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO6657285.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1872..4688
                     /gene="ileS"
                     /locus_tag="ACHKUB_004350"
     CDS             1872..4688
                     /gene="ileS"
                     /locus_tag="ACHKUB_004350"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO6657286.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4688..5182
                     /gene="lspA"
                     /locus_tag="ACHKUB_004351"
     CDS             4688..5182
                     /gene="lspA"
                     /locus_tag="ACHKUB_004351"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO6657287.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5270..5719
                     /gene="fkpB"
                     /locus_tag="ACHKUB_004352"
     CDS             5270..5719
                     /gene="fkpB"
                     /locus_tag="ACHKUB_004352"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6657288.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5721..6671
                     /gene="ispH"
                     /locus_tag="ACHKUB_004353"
                     /gene_synonym="lytB"
     CDS             5721..6671
                     /gene="ispH"
                     /locus_tag="ACHKUB_004353"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO6657289.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6737..7651
                     /gene="rihC"
                     /locus_tag="ACHKUB_004354"
     CDS             6737..7651
                     /gene="rihC"
                     /locus_tag="ACHKUB_004354"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO6657290.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7818..8639
                     /gene="dapB"
                     /locus_tag="ACHKUB_004355"
     CDS             7818..8639
                     /gene="dapB"
                     /locus_tag="ACHKUB_004355"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO6657291.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1970 a         2151 c         2463 g         2058 t
ORIGIN      
        1 aattcaaaaa tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa tatccacatt
       61 ctttgtaggg gagtctggag attgagtaga tattcttgtt cagaatgtat cagccgatgg
      121 ttctacgatt cttaagccac gaagagttca gaaagtgtaa cggcatgtct gcaaaagcca
      181 gcatcttttg actatctggc aaccggtagt gtgttctctc acgcatcata aaagcagcag
      241 gcataaaaaa acccgcttgc gcgggctttt tcacaaagct tcagcaaatt ggcgattaag
      301 ccagtttgtt gatctgtgca gtcaggttag ccttatgacg tgcagctttg tttttgtgga
      361 tcagaccttt agcagcctga cggtccacga tcggttgcat ttcgttaaat gctttctgtg
      421 cagcagcttt gtcgccagct tcgatagctg cgtatacttt cttgatgaaa gtacgcatca
      481 tagagcgacg gcttgcgttg tgcttacgag ccttttcaga ctgaatggcg cgcttcttag
      541 ctgatttgat attagccaag gtccaactcc caaatgtgtt ctatatggac aattcaaagg
      601 ccgaggaata tgccctttta gccttctttt gtcaatggat ttgtgcaaat aagcgccgtt
      661 aatgtgccgg cactcgttac gtagtgatgg cgcaggattc taccagcttg cggggtgtga
      721 atacagcttt tccgcgataa aaattgcagc aggcggtcac tttcttcccg tgatttgcgc
      781 catggcaatg aaaagccact tctttctgat ttcggtactc aatcgccggt taaccttgac
      841 cgctgtacaa ggtatactcg gacgattttc actgttttga gccagacatg aagctgatac
      901 gcggcataca taatctcagc caggccccgc aagaagggtg tgtgctgact attggtaatt
      961 tcgacggcgt gcatcgcggt catcgcgcgc tgttacaggg cttgcaggaa gaagggcgca
     1021 agcgcaactt accggtgatg gtgatgcttt ttgaacctca accactggaa ctgtttgcta
     1081 ccgataaagc cccggcacgg ctgacccggc tgcgggaaaa actgcgttac cttgcagagt
     1141 gtggcgttga ttacgtgctg tgcgtgcgtt tcgacaggcg tttcgcggcg ttaaccgcgc
     1201 aaaatttcat cagcgatctt ctggtgaagc atttgcgcgt aaaatttctt gccgtaggtg
     1261 atgatttccg ctttggcgct ggtcgtgaag gcgatttctt gttattacag aaagctggca
     1321 tggaatacgg cttcgatatc accagtacgc aaactttttg cgaaggtggc gtgcgtatca
     1381 gcagcaccgc tgtgcgtcag gcgctggcgg atgacaatct ggctctggca gaaagtttac
     1441 tggggcaccc gtttgccatc tccgggcgtg tagtccacgg tgatgaatta gggcgcacta
     1501 taggtttccc gacggcgaat gtaccgctac gccgtcaggt ttccccggtg aaaggggttt
     1561 atgcggtaga agtgctgggc ctcggtgaaa agccgttacc cggcgtggca aacattggaa
     1621 cacgcccaac ggttgccggt attcgccagc agctggaagt gcatttgtta gatgttgcaa
     1681 tggaccttta cggtcgccat atacaagtag tgctgcgtaa aaaaatacgc aatgagcagc
     1741 gatttgcgtc gctggacgaa ctgaaagcgc agattgcgcg tgatgaatta accgcccgcg
     1801 aattttttgg gctaacaaaa ccggcttaag cctgttatgt aatcaaaccg aaacacggaa
     1861 ccgagaatct gatgagtgac tataaatcaa ccctgaattt gccggaaaca gggttcccga
     1921 tgcgtggcga tctcgccaag cgcgaaccgg gaatgctggc gcgttggact gatgatgatc
     1981 tgtacggcat catccgtgcg gctaaaaaag gcaaaaaaac cttcattctg catgatggcc
     2041 ctccttatgc gaatggcagc attcatattg gtcactcggt taacaagatt ctgaaagaca
     2101 ttatcgtgaa gtccaaaggg ctttccggtt atgactcgcc gtatgtgcct ggctgggact
     2161 gccacggtct gccgatcgag ctgaaagtag agcaagaata cggtaagccg ggtgagaaat
     2221 tcaccgccgc cgagttccgc gccaagtgcc gcgaatacgc ggcgacccag gttgacggtc
     2281 aacgcaaaga ctttatccgt ctgggcgtgc tgggcgactg gtcgcacccg tacctgacca
     2341 tggacttcaa aactgaagcc aacatcatcc gcgcgctggg caaaatcatc ggcaacggtc
     2401 acctgcacaa aggcgcgaag ccagttcact ggtgcgttga ctgccgttct gcgctggcgg
     2461 aagcggaagt tgagtattac gacaaaactt ctccgtccat cgacgttgct tttcaggcgg
     2521 tcgatcagga tgcactgaaa gcaaaatttg ccgtaagcaa cgttaacggc ccaatctcgc
     2581 tggtgatctg gaccactacg ccgtggactc tgcctgcgaa ccgcgcaatc tctattgcac
     2641 ctgatttcga ctatgcgctg gtgcagatcg acggtcaggc cgtgattctg gcgaaagatc
     2701 tggttgaaag cgtaatgcag cgtatcggcg tgaccgatta caccattctc ggcacggtaa
     2761 aaggtgcgga gcttgagttg ctgcgcttta cccatccgtt tatgggcttc gacgttccgg
     2821 caatcctcgg cgatcacgtt accctggatg ccggtaccgg tgccgttcac accgcgcctg
     2881 gccacggccc ggacgactat gtgatcggtc agaaatacgg cctggaaacc gctaacccgg
     2941 ttggcccgga cggcacttat ctgccgggca cttatccgac gctggatggc gtgaacgtct
     3001 tcaaagcgaa cgacatcgtc gttgcgctgc tgcaggaaaa aggcgcgctg ctgcacgttg
     3061 agaaaatgca gcacagctat ccgtgctgct ggcgtcacaa aacgccgatc atcttccgcg
     3121 cgacgccgca gtggttcgtc agcatggatc agaaaggtct gcgtgcgcag tcactgaaag
     3181 agatcaaagg cgtgcagtgg atcccggact ggggccaggc gcgtatcgag tcgatggttg
     3241 ctaaccgtcc tgactggtgt atctcccgtc agcgcacctg gggcgtaccg atgtcactgt
     3301 tcgtgcacaa agacacggaa gagctgcatc cgcgtactct cgaactaatg gaagaagtgg
     3361 caaaacgcgt tgaagttgac ggcatccagg cgtggtggga tcttgatgcg aaagagatcc
     3421 tcggcgatga agctgatcag tacgtgaaag tgccggacac attggatgta tggtttgact
     3481 ccggatctac ccactcttct gttgttgacg tgcgtccgga atttgccggt cacgcagcgg
     3541 acatgtatct ggaaggttct gaccagcacc gtggttggtt catgtcttcc ctgatgatct
     3601 ccaccgcgat gaaaggcaaa gcgccgtatc gtcaggtact gacccacggc tttaccgtgg
     3661 atggtcaggg tcgcaagatg tctaaatcca tcggcaacac cgtttcgccg caggatgtga
     3721 tgaacaaact gggcgcggat attctgcgtc tgtgggtggc atcaactgac tacaccggtg
     3781 aaatggccgt ttctgatgag atcctgaaac gtgctgccga cagctatcgt cgtatccgta
     3841 acaccgcgcg cttcctgctg gcaaacctga acggttttga tccggcaaaa gatatggtga
     3901 aaccggaaga gatggtggta ctggatcgct gggccgtagg ttgtgcgaaa gcggcacagg
     3961 aagacatcct caaggcgtac gaagcatacg atttccacga agtggtacag cgtctgatgc
     4021 gcttctgctc cgttgagatg ggttccttct acctcgacat catcaaagac cgtcagtaca
     4081 ccgccaaagc ggacagtgtg gcgcgtcgta gctgccagac tgcgctgtat cacatcgcag
     4141 aagcgctggt gcgctggatg gcaccaatcc tctccttcac cgctgatgaa gtgtggggct
     4201 acctgccggg cgaacgtgaa aaatacgtct ttaccggtga gtggtacgaa ggcctgtttg
     4261 gtctggcaga cagtgaagcg atgaacgatg cgttctggga cgagctgttg aaagtgcgtg
     4321 gcgaagtgaa caaagtcatt gagcaagcgc gtgccgacaa gaaagtgggt ggctcgctgg
     4381 aagcggcagt aaccttgtat gcagaaccgg aactgtcggc gaaactgacc gcgctgggcg
     4441 atgaattacg atttgtcctg ttgacctccg gcgctaccgt tgcagactat aacgacgcac
     4501 ctgctgatgc tcagcagagc gaagtactca aagggctgaa agtcgcgttg agtaaagccg
     4561 aaggtgagaa gtgcccacgc tgctggcact acacccagga tgtcggcaag gtggcggaac
     4621 acgcagaaat ctgcggccgc tgtgtcagca acgtcgccgg tgacggtgaa aaacgtaagt
     4681 ttgcctgatg agtcaatcga tctgttcaac agggctacgc tggctgtggc tggtggtagt
     4741 cgtgctgatt atcgatctgg gcagcaaata cctgatcctc cagaactttg ctctggggga
     4801 tacggtcccg ctgttcccgt cgcttaatct gcattatgcg cgtaactatg gcgcggcgtt
     4861 tagtttcctt gccgatagcg gcggctggca gcgttggttc tttgccggta ttgcgattgg
     4921 tattagcgtg atcctggcag tgatgatgta tcgctcgaag gccacgcaga agctaaacaa
     4981 tatcgcttat gcgctgatta ttggcggcgc gctgggcaac ctgttcgacc gcctgtggca
     5041 cggcttcgtt gtcgatatga tcgacttcta cgtcggcgac tggcacttcg ccaccttcaa
     5101 ccttgccgat actgccattt gtgtcggtgc ggcactgatt gtgctggaag gttttttgcc
     5161 ttctaaagcg aaaaaacaat aataaaccct gccggataag gcctacagat tgctgcgaaa
     5221 tcgtaggccg catccggcaa aaatcctcaa aatataagag caaacctgca tgtctgaatc
     5281 tgtacagagc aatagcgccg tcctggtgca cttcacgcta aaactcgacg atggcaccac
     5341 cgccgagtct acccgcaaca acggtaaacc ggcgctgttc cgcctgggtg atgcttctct
     5401 ttctgaaggg ctggagcaac acctgttggg gctgaaagtg ggcgataaaa ccaccttctc
     5461 gttggagcca gatgcggcgt ttggcgtgcc gtcaccggac ctgattcagt acttctcccg
     5521 ccgtgaattt atggatgcag gcgagccaga aattggcgca atcatgcttt ttaccgcaat
     5581 ggatggcagt gagatgcctg gcgtgatccg cgaaattaac ggcgactcca ttaccgttga
     5641 tttcaaccat ccgctggccg ggcagaccgt tcattttgat attgaagtgc tggaaatcga
     5701 tccggcactg gaggcgtaac atgcagatcc tgttggccaa cccgcgtggt ttttgtgccg
     5761 gggtagaccg cgctatcagc attgttgaaa acgcgctggc catttacggc gcaccgatat
     5821 atgtccgtca cgaagtggta cataaccgct atgtggtcga tagcttgcgt gagcgtgggg
     5881 ctatctttat tgagcagatt agcgaagtac cggacggcgc gatcctgatt ttctccgcac
     5941 acggtgtttc tcaggcggta cgtaacgaag caaaaagtcg cgatttgacg gtatttgacg
     6001 cgacctgtcc gctggtgacc aaagtgcata tggaagtcgc ccgcgctagt cgccgtggcg
     6061 aagaatctat tctcatcggt cacgccgggc acccggaagt ggaaggcacg atgggtcagt
     6121 acagcaaccc ggaaggggga atgtatctgg ttgaatcacc agacgatgtg tggaaactga
     6181 cggtcaaaaa cgaagagaaa ctctccttta tgacccagac cacgctgtcg gtggatgaca
     6241 cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa aattgtcggt ccgcgcaaag
     6301 atgacatctg ctacgccacg actaaccgac aggaagcggt acgtgccctg gccgaacagg
     6361 cggaagttgt gctggtggtc ggttcgaaaa actcctctaa ctccaaccgt ctggcggagc
     6421 tggcccagcg tatgggcaaa agcgcgtttt tgattgatga tgcgaaagat atccaggaag
     6481 agtgggtgaa agaggttaaa tgcgtcggcg tgactgcggg cgcatcggct ccggatattc
     6541 tggtgcagaa tgtggtggca cgtttgcaac agctgggcgg tggtgaagcc attccgctgg
     6601 aaggacgtga agaaaacatt gttttcgaag tgccgaaaga gctgcgtgtc gatattcgtg
     6661 aagtcgatta agtcattagc agcctaagtt atgcgaaaat gccggtcttg ttaccggcat
     6721 tttttatgga gaaaacatgc gtttacctat cttcctcgat actgaccccg gcattgacga
     6781 tgccgtcgcc attgccgccg cgatttttgc acccgaactc gacctgcaac tgatgaccac
     6841 cgtcgcgggt aatgtctcgg ttgagaaaac tacccgcaat gccctgcaac tgctgcattt
     6901 ctggaatgcg gagattccgc tcgcccaagg ggccgctgtg ccgctggtac gcgcaccgcg
     6961 tgatgcggca tctgtgcacg gcgaatcggg aatggctggc tacgactttg ttgagcacaa
     7021 ccgaaagccg ctcgggatac cggcgtttct ggcgattcgg gatgccctga tgcgtgcacc
     7081 agagcctgtt accctggtgg ccattggtcc gttaaccaat attgcgctgt tactttcaca
     7141 atgcccggaa tgcaagccgt atattcgccg tctggtgatc atgggtggtt ctgccggacg
     7201 cggcaactgt acgccaaacg ccgagtttaa tatcgctgcc gatccagaag cggctgcctg
     7261 tgtcttccgt agcggaatcg aaatcgtcat gtgcggtctg gatgtcacca atcaggcaat
     7321 attaacccct gactatctcg ctactttgcc ggagttgaat cgcaccggaa aaatgcttca
     7381 tgcactgttt agccactacc gtagcggcag tatgcaaagc ggtctgcgaa tgcacgatct
     7441 ctgcgccatc gcctggctgg tgcgcccgga cttattcacc ctcaagccgt gttttgtcgc
     7501 ggtagaaact caaggcgaat ttacctcagg cacgacggtg gttgatatcg acggttgcct
     7561 gggcaaacca gccaatgtaa aggtggcatt ggatctggat gtgaaaggct tccagcagtg
     7621 ggtggctgag gtgctggctc tggcgtcgta acctgtcaca tgttatcggc atgcagtcat
     7681 tcatcgactc atgcctttca ctgatatccc tccctgttta tcattaattt ctaattatca
     7741 gcgtttttgg ctggcggcgt agcgatgcgc tggttactct gaaaacggtc tatgcaaatt
     7801 aacaaaagag aatagctatg catgatgcaa acatccgcgt tgccatcgcg ggagccgggg
     7861 ggcgtatggg ccgccagttg attcaggcgg cgctggcatt agaaggcgtg cagctgggcg
     7921 cggcgctgga gcgcgaagga tcttctttac tgggcagcga cgccggtgag ctggctggtg
     7981 cagggaaaac aggcgttacc gtgcaaagca gcctcgatgc ggtaaaagat gattttgatg
     8041 tgtttatcga ttttacccgt ccggaaggta cgctgaacca tctcgctttt tgtcgccagc
     8101 atggcaaagg gatggtgatc ggcactacgg ggtttgacga agccggtaaa caggcgattc
     8161 gtgacgccgc cgccgatatt gcgattgtct ttgccgccaa ttttagcgtt ggcgttaacg
     8221 tcatgcttaa gctgctggag aaagcagcca aagtgatggg tgactacacc gatatcgaaa
     8281 ttattgaagc acatcataga cataaagttg atgcgccgtc aggcaccgca ctggcaatgg
     8341 gagaggcgat cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc tacagtcgtg
     8401 aaggccacac cggtgaacgt gtgcctggca ccattggttt tgccaccgtg cgtgcaggtg
     8461 acatcgttgg cgaacatacc gcgatgtttg ccgatattgg cgagcgtctg gagatcaccc
     8521 ataaggcgtc cagccgcatg acatttgcta acggcgcggt aagatcggct ttgtggttga
     8581 gtggtaagga aagtggtctt tttgatatgc gagatgtgct tgatctcaat agtttgtaac
     8641 ca
//
LOCUS       DAZFRN010000183         8632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFRN010000183 DAZFRN010000000
VERSION     DAZFRN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.185"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(73..951)
                     /gene="glxR"
                     /locus_tag="ACHKUB_004356"
     CDS             complement(73..951)
                     /gene="glxR"
                     /locus_tag="ACHKUB_004356"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6657292.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1051..1827)
                     /gene="hyi"
                     /locus_tag="ACHKUB_004357"
     CDS             complement(1051..1827)
                     /gene="hyi"
                     /locus_tag="ACHKUB_004357"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO6657293.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1840..3621)
                     /gene="gcl"
                     /locus_tag="ACHKUB_004358"
     CDS             complement(1840..3621)
                     /gene="gcl"
                     /locus_tag="ACHKUB_004358"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO6657294.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3711..4526)
                     /gene="allR"
                     /locus_tag="ACHKUB_004359"
     CDS             complement(3711..4526)
                     /gene="allR"
                     /locus_tag="ACHKUB_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO6657295.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4604..5086)
                     /gene="allA"
                     /locus_tag="ACHKUB_004360"
     CDS             complement(4604..5086)
                     /gene="allA"
                     /locus_tag="ACHKUB_004360"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO6657296.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5316..6242
                     /gene="allS"
                     /locus_tag="ACHKUB_004361"
     CDS             5316..6242
                     /gene="allS"
                     /locus_tag="ACHKUB_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO6657297.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6311..7405
                     /gene="mnmH"
                     /locus_tag="ACHKUB_004362"
     CDS             6311..7405
                     /gene="mnmH"
                     /locus_tag="ACHKUB_004362"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO6657298.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQAIIELSQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            7521..7886
                     /gene="ylbG"
                     /locus_tag="ACHKUB_004363"
                     /pseudo
     CDS             7521..7886
                     /gene="ylbG"
                     /locus_tag="ACHKUB_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(8407..>8632)
                     /locus_tag="ACHKUB_004364"
     CDS             complement(8407..>8632)
                     /locus_tag="ACHKUB_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657299.1"
                     /translation="DDGGRFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGD
                     GVNLFFVGEINVTHYFITNIGAGLPDACAE"
BASE COUNT         2195 a         2255 c         2029 g         2153 t
ORIGIN      
        1 tctttggctg ctttcttttt taatattgct ggccgattca ttttctgata tcggccattt
       61 ttattgcggg tatcaggcca gtttatggtt agccattaat tccagcgcct gcactaacgc
      121 agagtgatcc aactggctgc caccgtttgc cgcacaggta ttaaataact cctggcaggt
      181 cgcagtgttt ggcaggttca gcgcaagtgc tttcgcactt tgcagtgcca ggttgagatc
      241 tttctggtgc agagcgattt tgaagcccgg attaaaggtg cgtttaatca tacgctcgcc
      301 atgaacttcc agaatacgtg aggaagcaaa gccgcccatc agcgcctggc gcacacgtac
      361 cgggtccgca ccggcttttg aagcaaatag cagggcttca gaaaccgctt caatattgag
      421 cgccacgata atctgatttg ccactttgca ggtttgaccg tcgccgttac cgcccacgag
      481 ggtgatattt ttaccgagca gttcaaacag cggtttaaca cgttcaaata ccgcttcatc
      541 accgccaacc ataatcgaca acgtcccttc acgcgcaccg atttcaccgc cggagactgg
      601 cgcatcgaga taatcgccgc ccagttcatt cacctgacga gcgaaacgct tagtttcaat
      661 cggggaaatg gagctcatgt caacaatggt tttgcccttc agcgaggctt tggtacaacc
      721 attttcaccg aacagaactt cttcaacctg tggtgtatct ggcaccataa taaaaatgat
      781 gtccgatgct tccgttacct ggcgcgcagt ttcaacactg acggcaccca gtgacagtaa
      841 ttcatcagca accggtccaa tggttgtgac atgcagttga tgaccggcac gcgccagatt
      901 aatggccatc ggtgtaccca taatgcctaa gccaataaat cccagtttca taattaacct
      961 cttttaaatt tcgcttttcc tgaattcaga caacacgatc ccctgccaac ttagcggcct
     1021 aaaaaagcat tgcctgaaca gcgttacgtt ttaacggtac ggatccatcc agcgtaaacc
     1081 ggcttccgtg gtggtttggg gtttatattc acacccaacc caaccgttgt agtcagaatt
     1141 ttcgattacc ttaaagagat aatcataatt aatttctccg gtccccggtt cgccgcgatg
     1201 cggattatca gcaatttgca ggtggccaat tttatcggcc cactgagtca tggtgttggt
     1261 taattcacct tccatccgct gcatgtgata aatgtcatac tgaattttca aattgcagca
     1321 accaacatca tcaatcaatt tcagcgcctg ccgagttccg gtgagatgga aaccaggaat
     1381 atcaaaatgg ttaataggtt caatcagtaa taaaatatct tctttcatca gcatattcgc
     1441 ggcataacgc aggttttcta caagcgttgc gtgaatctgt tcactgctga aaccagccgg
     1501 cgttttaccg accagacagt taattttttt attacccagc gcacgggcat aacgaatcgc
     1561 tgctgctacg ccatcccgaa actcttcttc acggccagga atacaggcaa taccgcgctc
     1621 ccccgccgcc cagtcacccg ccggtaaatt gtgcagcgta tgttcgagtt tattactcgc
     1681 cagcacctgt tttaattctt caatgtcgta gtcataagga aacataaatt caacgccgcg
     1741 aaaaccacac tgcgcagctt tctcaaaacg ggcgagaaaa tcatattctc caaataacat
     1801 cgataaatta gcagagaaac gtaacattat ttatctccct tattcatagt gcatgaagca
     1861 ggtttcagtc ggtgcgtccg ctgcgttatc ggcgatatct tcaaattcca tgacgttatc
     1921 cagttcgctg cccatcgaaa tattggtcac acgctcgaga ataacttcca cgactaccgg
     1981 tacccgatat tgcgccatta aggctttcgc ctgttcaaag gctggcgcaa tatcttccgg
     2041 tttgaagacg cgaatcgctt tacaacctaa accttccgct acttttacgt ggtcaacacc
     2101 gtagccattc acttcactgg agttgatatt ctcgaaagcg agttgcacgc agtagtccat
     2161 atcaaacgcg cgctgcgact ggcgaatcag ccccagataa gcgttattga ccagtacatg
     2221 gatgtacgga atgttgaact gtgcgccaac agctaactct tcaatcagga actggaagtc
     2281 aaagtcgcca gaaatcgcca ccacattgcg tttcggatca gcggcacaaa cccctagcgc
     2341 agccggaatc gtccagccta acggaccagc ctgaccacag ttgatccagt ggcggtcttt
     2401 aaagacatgc agcatttgtg ccgcagcgat ttgtgacaga ccaatggtgg tgacatagca
     2461 aacatcgcga ccaaaggctt tgttcatctc ttcatacacg cgctgcggtt tcaccggcac
     2521 gttgtcgaaa tgggttttgc gcagcaaagt gcgtttgcgc tgctggcagt cggcgaccca
     2581 ttctttacga cacggcagac gacccgcttt ttgcatctcc tgcgccactt caaccagcag
     2641 tgtcagcgcc gctttagcat cagagacaat gccgagatcc ggacacagca cgcgaccaat
     2701 ttgcgtcggc tcaatatcga tatgaacgat tttgcgccct tcggtgtatt tctctaccga
     2761 accggtatga cggttagcaa aacggttacc gataccaaac accatgtcgg acgccagcaa
     2821 cgttgcgtta ccgtaacgat gcgcggtttg cagacccacc atcccggcca tcagttcatg
     2881 atcgtccggg atacagcccc agcccatcag cgttgggatc accggaacgc tggtcagttc
     2941 agcaaactgt tgtaacagtg cagctgcgtc agcattaatt accccgcccc cggcaacaat
     3001 caccggacgt tcggcctgga ttaacatttc tacagctttt tcgatctgca tacggctggc
     3061 agcaggtttg tagaccggca gcggttcgta catgtcagga tcaaactcga tttccgcaac
     3121 ctgaacgtcg aacggtaaat ccaccagtac cggacccgga cgaccagaac gcatcaggtg
     3181 aaatgcctgt tgcagcacgc gaggcaccag cgccgcttca cgaactgtaa ccgccatttt
     3241 gctgaccggt ttagcaattg cttcaatatc tacggcctga aaatcttctt tatgcagacg
     3301 ggcgcgcggt gcctggccgg taatgcacag aataggaatg gaatcagcag aagcggaata
     3361 gagcgcggtg atcatgtccg tgcccgcagg accggaagtc cccagacata cgccgatatt
     3421 tcctgccgtt gcgcgggtat aaccttccgc catgtgcgaa gcaccttcca catgacgcgc
     3481 cagaatgtga cgaataccgc cgtgcttacg catcgctgag tagaacggat tgattgcagc
     3541 tcccggaaca ccgaaggcgg tggtgatacc ttctttctcc agcacataca ttgccgcgtc
     3601 aacggctctc atttttgcca ttttattcct acctctattt attggaaaat ttttccaact
     3661 ttcaaatttt gtcaaaaaaa cccgctccgc agagcgggat gcgacagaca ttatggatgt
     3721 gctttcagtc ccaacgccgt gctgatatcg cgggcggtgt ctctgaccag ctcaccctga
     3781 ctgacaaaac gatcttctgt cagtcttgat gaaggcccgg agatggagat agcggcaaca
     3841 acgctaccga catcatcgta aattgctgaa gctatgcaat tcagacctac aacatgctct
     3901 tctttatcta cggtatagcc cagttcacgc gcttgttcca ggtccttcag caaggtgggc
     3961 atatccacaa gcgtagttgg cgtaaactgc tgcaaaccgg tttgcagaat gatgctcatc
     4021 aactcctctt ccgccagcgg ataaagcagc gctttgcccg caccggaagc atgcagcggc
     4081 agacgactgc ccagtggcgc acacatcctg accattgatt tacactctaa ctgaccaatt
     4141 aatactgctt cattgccgtt acggatcgcg acattgaccg tttcgccgga aagtaacatc
     4201 aggcggcgca taaacggccc ggcgacggag aggacatcgc ggttatggat gtacgccgca
     4261 ccgacgttaa agacacctaa tcctatatgc caccagccta attgactgtc ctgatagaca
     4321 aaatccgctg cctgtaaaac cttcagcaag cgaaaggtcg tggagagcgg caaatccaga
     4381 ttgagagaaa tatcgctaac cgacgaactt cccccacttt tttccagata ttgcagaatc
     4441 gcaattcccc gctctaacgc ctgtgcgccc ttctgtgcca caggctccgc ctgtcctggc
     4501 ctgccgcgcc gtctaacttc cgtcatacct acgttaactc cgacctacaa gtgagcataa
     4561 acaatgaaca attagtttat cttatgcaag tcggttgcag gcgtcacgca aaacagagtt
     4621 cctgttcagg aatactttca acatcacagt tgtcactgcc gccgcgatcg atggtcagaa
     4681 aatcggtgac gcgctgccag gcgaaaagtg ggtgatgcca gacgttacga tggtaattca
     4741 ctccctgttc gccgttggtg ataaacgccc gcagcgttga caggtctggt ttgtcgtcgc
     4801 ctaacgccac gaccaccaca aacacttcac ctttcatcgg gataaaggcc tgagtgccca
     4861 gcggatgacg ttcgagttcg taaatggtca gcggcagatt cgccggttgc gcgcggttaa
     4921 tgctgataag cgtacggtct tgctcaagaa tctcaaccag cgccaaatcg tggtaacgct
     4981 ccaccaggcc attgttaata tggaaaaaat cccgttgctg agtttcgatt acgtcgccat
     5041 aagcactaaa ggcttcctga cttaacggta atacctgaag tttcatcttt ttgtccttgt
     5101 aacataattc cgttcaacca tcgccgaccg gtcagcttgg tttcaataac aggcaatcta
     5161 aaaccttaag agaacaacat caatacaaaa gttggaatat ttttccaaaa ctagatttag
     5221 tccacaaatt gaacagggtt atcatgtaaa aatactgata aatcagagga taagaaaatc
     5281 acgcaacttg cggatttaaa aggggatacc tgaagatgtt cgatccagaa accttgcgga
     5341 ctttcattgc ggttgctgaa acaggaagtt tttcaaaagc ggcagaacga ttatgtaaaa
     5401 ccacggcgac gatcagttat cgcattaaac ttctggaaga gaataccgga gtagcgctgt
     5461 ttttccgtac gactcgtagc gtaacgttga cagcggctgg cgagcatcta ctttcccagg
     5521 ccagagactg gctgagctgg ctggaaagta tgccaagcga gctgcaacag gtgaatgatg
     5581 gcgtggaacg ccaggtgaat attgtcatca acaacctgct ctacaacccc caggccgtcg
     5641 cccagttgct ggcgtggctg aatgaacgtt acccctttac ccagtttcac atctcccgac
     5701 aaatctatat gggcgtctgg gattcgctat tgtacgaagg tttttcactg gctatcggcg
     5761 tcacgggaac cgaggcgctg gcaaatacct ttagtcttga tcccttagga tcggtgcaat
     5821 ggcgctttgt catggcggcg gatcatccgc tggcgaacgt tgaagagccg ctaacagaag
     5881 cgcagttgcg gcgctttccg gcggtcaata ttgaagacag cgcccgcacc ttaaccaaac
     5941 gcgtcgcctg gcgattgcca gggcaaaaag agattattgt tcctgatatg gaaacgaaaa
     6001 tcgccgccca tcttgcgggc gttggcattg gttttttgcc aaaatcgctt tgccagtcaa
     6061 tgatcgataa tcaacaactg gttagccggg taatcccaac gatgcgccct ccttcgccat
     6121 tgagtctggc atggcgcaaa tttggcagcg gcaaagcggt agaagatatt gtgaccttgt
     6181 ttacccagcg caggccggaa atcagcggat ttttagaaat tttcggcaac ccacgcagtt
     6241 aaaaatcaag tttccttgcg cgttggcgcg taaaatagcg cgcaagctga tttgaggttc
     6301 acttttaact atgcaagaga gacacacgga acaggactat cgtgccctgc tgattgctga
     6361 tacgcccatt attgatgttc gcgcccctat cgagtttgag caaggcgcaa tgcccgccgc
     6421 tatcaatctg ccgttaatga ataacgatga acgcgccgcc gttggcacct gctataaaca
     6481 gcaaggctca gacgcagcgc tggcgctggg gcataaactg gtggcgggtg aaattcgtca
     6541 gcagcgcatg gacgcctggc gggcagcgtg cctgcaaaat ccgcaaggta ttctctgctg
     6601 cgcccgtggc ggtcaacgtt cacatattgt gcaacgctgg ctgcatgaag cggggattga
     6661 ttatccgctg gtggaaggcg gttataaggc actgcgccag accgcgattc aggcgatcat
     6721 tgaactgtca caaaaaccga tagtgctgat tggcggttgt accggcagcg gtaaaacgct
     6781 attagtgcaa caacagccaa acggtgttga tctggaaggg ctggcgcgtc atcgcggttc
     6841 ggcgtttggt cgcacgttac aaccacaact tagccaggcg agttttgaaa acctgctggc
     6901 tgccgaaatg ctaaaaaccg acgcccgtca ggatttgcgc ctgtgggtgc tggaagatga
     6961 aagccggatg atcggttcga atcacctgcc ggaatgtctg cgcgagcgaa tgactcaggc
     7021 ggcgattgcg gtggtagaag atccgtttga gatccgtctt gaacgcctga acgaagagta
     7081 tttcttgcgt atgcatcatg attttaccca cgcttatggc gacgaacagg gctggcagga
     7141 atattgcgaa tacctgcatc acggactttc ggcgattaag cgtcggctgg ggttacagcg
     7201 ctataacgaa ctcgctgccc agctggatac ggcgctgaca acgcaactca ccaccggcag
     7261 caccgacggt catctggcct ggctggtgcc gttacttaaa gaatattacg acccaatgta
     7321 tcgctatcag cttgagaaaa aagcggaaaa agttgtcttt cgcggtgagt gggcggaagt
     7381 ggcggaatgg gttaaggcgc ggtaatgagg gacgtatttg tattagtcgc gacttgatct
     7441 gacacatggc cttgaaaggt tgagagttac cggttttgat atgagtgtcg aatccttatc
     7501 caaaacaaga ggtaactctc atgcttcata ctgccaatcc cgtcatcaaa cacaaagccg
     7561 gtttgctcaa tctggctgaa gaactcagca acgtatcaaa agcctgtaaa atcatgggcg
     7621 tttcgcgtga tacgttttat cgttaccgcg aactggtcgc tgaaggcggt gtggatgcgc
     7681 agattaatcg tagtcgccac gctcctaacc ttaagaaccg taccgatgag gcaactgaac
     7741 aggctgttgt tgattacgcc gtcgctttcc cgacacatgg tcagcaccgg gccagcaacg
     7801 agctgcgtaa acagggggtt tttatctccg acagtggtgt ccgttctgtc tggttactcc
     7861 ataaccttga gaatctcaaa agacgatact gaacaaattt tatcaggtga cgttccgtaa
     7921 aaagttgtat ggagttctcg atgcagtcgg cgcgactatc agtacccccg ctgaaccctg
     7981 gactatcggg ccgccgtact ggggcatgtc accctggcgt gccgctggtt ttccgctaat
     8041 ggtttatttc ctctttcttt cccggcaaga atttatttta atccggaagg attatttatt
     8101 ttcagatagg gactcacatt aaacacgtaa cattttaatt aatgcatggc tgaatatgta
     8161 ttacacaatg tcagatatcg taaaagacca gtgcaattct atgtaaactc tctgactgtt
     8221 cattttattt gttgtttcag ggtcggaact caccctcaat aaacaactga atacgagtag
     8281 atattcatcg tctgagctat atggctttac acaatagccg acatgtcttt tctcagtcag
     8341 gaattttact tttcctcact acgaagttga cgacttcgat atgggataga ctcttaattc
     8401 aagcaattac tctgcacaag catcaggcaa tccagctcca atatttgtta taaaataatg
     8461 cgtaacatta atttcaccaa caaaaaaaag gtttacacca tccccttgcc ctggtatctt
     8521 accatattta caccacactt tttttgtatc tggatcattc tttgatacga ttttcgccac
     8581 ttgttccctg gccctcactg tcaatgtttt cttaaaccta cccccatcat ca
//
LOCUS       DAZFRN010000184         8568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFRN010000184 DAZFRN010000000
VERSION     DAZFRN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.186"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            3..1235
                     /locus_tag="ACHKUB_004365"
     CDS             3..1235
                     /locus_tag="ACHKUB_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO6657300.1"
                     /translation="MTKTLLSSSLVVGLLSVSSFTRANEYSYSALDVLGKHGSTYTIV
                     YNSDSDTAYVKWKKVPSGVEGIKDGDIVQGLSKINKDGTVEVTTKDGTSTYRVYDREF
                     HNLMNSLVKQDKENTLGKKEGTQTDLVSDADIRNLFGRIAAISEMKTNVGLDEYGRVA
                     TSAESIKERVSLQTYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIAKDVE
                     EQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGATKTI
                     AVNDGLVALSGRTDRIDAAVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIREN
                     HEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAG
                     VAASDGDVSYNVGVNFEF"
     gene            1322..1795
                     /locus_tag="ACHKUB_004366"
     CDS             1322..1795
                     /locus_tag="ACHKUB_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657301.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSAKHLVGRA"
     gene            2167..6159
                     /locus_tag="ACHKUB_004367"
     CDS             2167..6159
                     /locus_tag="ACHKUB_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6657302.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSQGSGE
                     GTLSQSGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRPE
                     GNQTWVGGGIIVNDGHRVDWMVNGLAGDALHKIGKGILVVAGSGENPGALNTGDGTVI
                     LAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDMAFN
                     RLAAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDG
                     GADMSGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESR
                     TFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGT
                     SAVGGASGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSS
                     ALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSG
                     EYDFMTGAAGLLRGYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFH
                     TLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLV
                     TAPAGTSADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVVTRQDA
                     ETPNPVEAVSPLPSPVSAPSPAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVN
                     SSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSD
                     NYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSGTFSGKTKSVGAGLYASALFDSG
                     AYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELV
                     YGAVSGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFD
                     LLANGETVLRDASGEKRIKGGKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDN
                     AVNASFRYSF"
     gene            complement(6582..6740)
                     /locus_tag="ACHKUB_004368"
                     /pseudo
     CDS             complement(6582..6740)
                     /locus_tag="ACHKUB_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            6888..7013
                     /locus_tag="ACHKUB_004369"
                     /pseudo
     CDS             6888..7013
                     /locus_tag="ACHKUB_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(7073..7579)
                     /gene="yciE"
                     /locus_tag="ACHKUB_004370"
     CDS             complement(7073..7579)
                     /gene="yciE"
                     /locus_tag="ACHKUB_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6657303.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(7625..8125)
                     /gene="yciF"
                     /locus_tag="ACHKUB_004371"
     CDS             complement(7625..8125)
                     /gene="yciF"
                     /locus_tag="ACHKUB_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6657304.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(8211..8390)
                     /gene="yciG"
                     /locus_tag="ACHKUB_004372"
     CDS             complement(8211..8390)
                     /gene="yciG"
                     /locus_tag="ACHKUB_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6657305.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         2160 a         1771 c         2422 g         2215 t
ORIGIN      
        1 aaatcacaaa gacacttctg tcatcttctc tggttgtagg tctgttgagt gttagttctt
       61 ttacacgtgc aaatgaatat agctacagcg ccttggatgt gttaggtaaa cacggaagta
      121 cttacaccat tgtttataat agtgattctg ataccgctta tgttaaatgg aaaaaagtac
      181 ctagcggtgt tgaggggatt aaagatggag atattgtaca aggattatca aaaattaata
      241 aagatgggac tgtagaagtt actaccaagg atggcactag tacatatcgt gtgtatgacc
      301 gggagtttca taacctaatg aattcactgg tcaagcagga taaagaaaac actcttggaa
      361 agaaagaggg tacccaaaca gaccttgtat ctgatgctga tattcggaat ctttttggca
      421 gaattgctgc gatctctgaa atgaaaacta atgttggtct ggatgaatat gggcgagtgg
      481 caactagtgc agagagtatt aaagaacgcg tgtctttgca gacttatctg gctacagagg
      541 ctgcaaatag cgcaattggc ctagataaaa acggtaagct aactctgaat gagacagata
      601 ttgtaacgcc gttacgtatc aatgacggcg ttgtcactat agcgaaagat gtggaagaac
      661 agaaagaagg gctggcgatc cagaagaaag gactgtctga gctttcatcc cgcacagata
      721 tcattgatgc agcaacggct gcgaatacga ataaaattga ttcccgaacc accgttggtg
      781 tgaatgcgga cggaaccctg accagcgctg atggcgctac aaaaacgatt gctgttaatg
      841 acggtctcgt tgctctgtcc ggcagaacag accgtattga tgctgcagtt ggtagcgttg
      901 accgccgggt tactaaaaat acacaggcta ttcagtccaa tactcgccag ttgcaggaac
      961 ataatgcacg cctgaacagc cagcagcgtc aaattcgtga gaatcacgaa gagatgaagc
     1021 gtgcagcagc acagagtgca gcgctggcgg ggcttttcca gccgtacagt gtggggaaat
     1081 tcaacgccac tgctgccctg ggggggtaca gtgataaaca ggctgttgcc gtgggtgttg
     1141 gttaccgttt caatgagcag acagcagcga aagcaggtgt ggcagcgagt gatggcgatg
     1201 tgtcctacaa cgtaggtgtg aactttgagt tttaattgct gacagtgaca tgaagcatga
     1261 cggcaggcgt ggagttttgc ctgccgtttt ttctcatgtg agcatgaaac aggtgacagc
     1321 gatgaaagtg attctggcga cccgcaaccg ttatctggag tatggcctgc agcagatgct
     1381 tgaggggtac aggataatac tggcgaggga gttttttaca ccagagaaca ggaagagtgt
     1441 accggcacat gatgagtcat gggtgataat ctgtgatgcc ctccttggca ggctgatgtg
     1501 ctgcatgttt cagggacgcc gttatcttca gattgatgcg gaagatgtta cggggcgcct
     1561 ggagacgtac aggaaaatcc ggaatggtga gtgggtacat aatacgtatg cgcgacctct
     1621 aacgatgtcc gagatggtgg tgatgtttgg atacgtgtac cgggagtcaa agccctgcca
     1681 tcttgcccgt gaaatgggga taaacacgaa gacggtgaac acctttctgt atatgggact
     1741 tggtaaaaac ggtcttaagt acaggagtgc gaagcacctg gttggacgtg cgtagccgga
     1801 atgcatattc gtgagtcggg taatgagagt tgaacggtgg cagtgagaat ggaacagaaa
     1861 aaaatcagat gcggaaggcg gaaatacaca gcgggtgcga tgtgcttcat gctgtttgta
     1921 tggctgggtg gtgtggtgac attctgtgtg ctggccgctc tgataatgag gtgaaaacgg
     1981 aagagagttt acttccttaa ttcgaacttt ctgataactg agaacttgtt aaatgctcgt
     2041 gggtttatat atttaaatca attggttaat gctgacttca tctttatcag tcaaagtttg
     2101 ttttagtatg atgattgtgc ccactgttta ttgttgtccg ggagaaaata cagggaattc
     2161 cagggaatga acaaaatata ctcactcaga gttaatgaca gaaaagaact ggttgctgta
     2221 ccggaaacgg tggggggcag gaaaaagtcg tccggaattc ccggtgcggg gatgctttcc
     2281 cgtcttcttc tggcctcagg agccgttgcc ggcgttctgt tttcttctcc tgcgctggca
     2341 tccgttgtcg ggaatactct gccatggcag acctacaggg attttgctga aaacaaaggg
     2401 gcgtttcatg ccggggccac aaatattccg ctttatggta agaatggggc tgtcagtggg
     2461 cggctggaca aggctccgat gatggatttc agtgttgtgg accagatact gggggtggca
     2521 acactgattt ctccgcagta tgtggcgggg gttaagcata acggcagcta caatacggtg
     2581 cgttttggtt atgcggacga tacgacatat cgccttgttg accgtaatga gcactggcgt
     2641 gattttcata ctccccggct gaacaaactg gtgacagaag ttgccccggt gagcgtcacg
     2701 gatgccggaa ccggcaaggg ggtataccag aaccgaagtc gttatccggt gttttatcgt
     2761 atggggagtg gaactcagta caccggagct gcttccggtg cgctgaccag aatagcggat
     2821 gcttacgtct ggaggactgg cgggacggtg ggaagtccgg tcatttctga ctggagtctg
     2881 gtgtcaaacc cgggaaatct ttaccagggt gttaatggtc cgctggcgtc ttacgggaca
     2941 tcaggagaca gtggctcacc gctttttgcc tgggatgcgg tgaagaagca gtgggtattt
     3001 gttgctgtac ttcatggtta tgccggtgag aacgggaaga cgaactggtt tacggtgatt
     3061 cctgcagggg atgtgaataa cacgataaag caggacaatt caggcacagt tgtgccggca
     3121 gttgccggtg gtgacattgt gtggaagtac agtcagggaa gcggagaggg aaccctttct
     3181 cagagcgggc aggtatggaa gatgaacggc ttcaggggag ggagtctgaa tgacgggaaa
     3241 gatatcacct ttgggggtaa gggcacggtt gttctgaagg atgatgttgt ccagggggct
     3301 ggttcgctca cgtttaatgg tgattatacg gtcagaccgg aagggaatca gacctgggtg
     3361 ggcgggggaa tcatcgtgaa cgacgggcac cgtgttgact ggatggtgaa cggacttgct
     3421 ggagacgcac ttcataaaat tggcaaggga atactggtgg tggctggcag tggtgaaaat
     3481 cccggcgccc tgaatacggg ggacggaacg gtcattctgg cacagaaggc tgatgctgca
     3541 ggcagggtcc gggcattcag tgaagtcagt attgtcagcg gacgaccagt tgtggtgctg
     3601 caggacagtc atcagattga aggggaccgc attcgctggg gataccgggg aggcacgctt
     3661 gatatcaacg gaaatgacat ggccttcaac cgcctggctg cggctgatga aggggcagta
     3721 ctcacaagcc gggcgcgtcc tgccacagtc cgtctggatt tcagccagtc agggcagaag
     3781 gccgtgatgt ggcatggtca ttttaccggt aacctcagcg tcctgaacaa cacgtcatct
     3841 gcggtggatt ttatcatgga tggcggagcg gatatgtccg gcagttttac acagcagggc
     3901 gggggactgt atatccaggg gcatccggtt gttcatgctg tcagcagtga ggcggttgcg
     3961 actgcattgc gaaagcaggg tgataattct gttctgacgc agccggtatc atttgctcag
     4021 aaggactggg agagcagaac gttcagcatc ggtcaactga aactgaaggg ggcggcattc
     4081 agcctttccc gcaatgcgac actgacggga gatattgatg ctgataatgc caccatggtg
     4141 ctgggaagtg actcgctata tctggatatg aaggacggca cggggagcag cagtgcgccg
     4201 gttaagggga cgtctgccgt gggcggtgca tccggtagca gtacatttcg cgggaatgtg
     4261 aatatgcgtc attctgccct gacggtccgc gaccatttta ctggcagtat aacggcatcg
     4321 gacagccgga tagctgtgag ttcagagaat gtgcgtctgg aagggaattc ccgcctgacc
     4381 tcttctgcac ttactgtcag tgacggcggg cgtttacatg tgaagggggg actggagaca
     4441 gatggtggtg tgacactgga cagaggaacc cttttggtgg acgggggaag tgtcaggaat
     4501 gatgtatatg aacgtctgct ggcctggtct gaggagcgtg gcgggctgaa cggaagcggt
     4561 gagtatgatt ttatgacggg ggcagctggt ctcctgcggg gttatgtcag agggagtgca
     4621 ggtaatgtga atctgcagaa tgcggcctgg atgatgacgg gaaactcatc cgtgaagcat
     4681 cttgagtcat cgggaagtgc tctgtatttc agcaggccgg gcggggagtt ccataccctg
     4741 acggcaggca gtatggatat cagcgacagt gttctggtga tgcggacgga ccttaaccac
     4801 tcagaccagc ttcgggtgac agaatcgctg cggggtaaga ataacctgtt gttggttgat
     4861 tttactgagc gttctgacgg gcagaaggcg ctgaatatcc ctctggtgac ggcgccggcc
     4921 ggtaccagtg cggatgtgtt cagtgtgaaa acgcgtgata ccggcttcag tcatatcaca
     4981 cctgttgtca gggctgaaca ggggacgggc ggcacggcct ggcagctgaa tgttgtgcag
     5041 ccggaaactg ccgcagagcc tgtggtgacg cggcaggatg cagagacgcc gaatcctgtg
     5101 gaagctgtat caccgctccc ttcaccggtt tctgctccgt caccggctcc ggaggcgtca
     5161 gtgacggatg tactgaccgg agagaatgcg ggggaatcag gtgagtaccg tgatgagacc
     5221 cggtggctgc tgacaggata ccggagtacg gttaatagct ccgcggtcag ggatgcgggg
     5281 atgctgatgt caatggggca ccgtaacttc attaatgagg tcaacaacct gaacaaacgt
     5341 atgggtgacc tgcgggacat caacggcgag gccggtgcat gggcccgtat catgagcgga
     5401 accggctctg ccggcggtgg cttcagtgac aactacacac acgttcaggt gggtgcggat
     5461 aaaaaacacg aactggacgg tctggacctc ttcaccgggg tgaccatgac atataccgac
     5521 agcaacgccg gcagcggcac cttcagtggt aaaacgaagt cagtgggtgc gggtctgtat
     5581 gcttcagccc tgtttgattc cggggcctat atcgacctga ttggtaagta tgttcaccat
     5641 gataatgagt atacggcgtc ctttgccgga ctcggcacaa aagactacag ctctcattcc
     5701 tggtatgccg gtgctgaagt gggttaccgc tatcatgtca cagaggatgc ctggattgag
     5761 ccacaggcgg aactggtcta tggtgctgta tccggtaaac agttcagctg gaaggaccag
     5821 ggaatgagcc tgtctatgaa ggacaaggac tacaatccgc tgattggccg tactggtgtg
     5881 gatgtgggta aatcgttctc cggtaaggac tggaaagtga cggcacgtgc tggtcttggc
     5941 tatcagtttg acctgctggc gaacggagaa acggtactgc gtgatgcatc cggagagaag
     6001 cgtattaagg gcgggaaaga cggtcgcatg ctgatgtctg tgggtctgaa cgcagaggtt
     6061 cgtgacaaca tccgttttgg tctggagttt gagaaatcag cctttggtaa gtacaacgtt
     6121 gataatgctg tcaacgccag cttccgttat tcattctgac ggttgttact gaaggggatt
     6181 atggcctgac ggctccctgt aaataaagac aggcacagaa gattgtgagt tacttagtgt
     6241 tccaccaaaa agtaaggtcg caactctgtg cctgcttcgt aagtgtacag tgagggccat
     6301 atggtcataa ctgaagcatc cgacgatgtg gtgtctaccc aaaaagcgac ctgtgaatat
     6361 taggctggtt aagtgcgtat tttcatggaa ggagatcacc caatagcccc gatctccttt
     6421 atgttaatac gcattaagcg ggtaaaacag gaagattaag aaagggagag tttttcgact
     6481 ccccaaccaa gagaagcagc ttataactac cagatagttg ttatgtcata cgtgacggat
     6541 aatgtcataa aaaatatgtc attgccgtga ttataatcgc gttattggat aaagcagtct
     6601 tcagcggttt cccaggcttc ctgcagcgtt tcctgtacaa attcgtgcgc ggattcttta
     6661 tttacaactc ttaaaatggt gagtccatca ttgctggtgg ttctgacaat cacgtccaca
     6721 tggtcatatc cgttatttat ggaatgacta taagggggga taagcctctg caaaacaaaa
     6781 aagactttaa caaaatcaat cagttaaaaa cacagaaaaa cggtatgaaa aatcaccata
     6841 aaagtttgtt tttcctttta taaatagctg cttatcacat tttttataat ctaccagtgt
     6901 ggtacatgga tatcgatacc accgccaatt ataagctagg cggtgcacag caacacgata
     6961 gcgtacgcct cgatccgtgg gtgtttatgt tctcagcagg atatcgtttt taattccgca
     7021 caaaaacgac cccgtaatat acggggtcaa taaggacatg gtataaaggg tattatttct
     7081 tcgcttctac gccatcagtt tcagaacgaa ttaaaaattt ctcagttgtt tgcggaatat
     7141 gctgaatcag ccagtcggcc atttgctttt cctcatttaa aatcgcttcg atgatcggaa
     7201 ttgagactgt atcaccggca ttttttgctg ctgctaatag tgaggtgtaa caggcgattt
     7261 caaattgctc gaagacatat ccgctaatag agcctttaac tatttcatca gaagggaata
     7321 taccaccgat tgactgccca agcgcagcca ttttactcat ggaatcttta atgactgaac
     7381 gtgaaatgtc attacgatca agaatagttt ccagttgaac aatctggttt ttggtttcac
     7441 taagatgttg ttcaatacga gcgcgtagtt caggataatt atctatacga ctggccatgg
     7501 attcaagcat agattcggct tgcttttcca ttgcgtgggc gtcacgtaac cagtcatgat
     7561 aatgttcaat acgattcatg aaaatgtcct ctttataaaa tgactaaaag ttaaaattca
     7621 tatttcaggc tttattttcg gctttcttat ttacgttatt aagggccaga tcagtcagtt
     7681 tgatgtcggt ggccttttct tcttccaggg tttctttcaa aagcttcgct gctttacggt
     7741 aacctaattg ttcagccagc gtcgctaatg tcccgtaact ggcaatctca taatgctcga
     7801 ctttctgtgc tgcggcaatc agtgcggcat cacgcacttc gtttttctcg gtactttcga
     7861 tgacctcatt agcttcttca ataagacctt ccattgccac acatttcatg cgcttaattt
     7921 tcaggttcga ttcggactcc acaacttgat caatacgttc aatctgtcca tgagtttcct
     7981 cgaggtgcgc atgaaaagcc tgacttaatt tttcatttga tgttgctctt gcgagttttg
     8041 ccagtgcccg ggttaattgt ttttctgcgc tgtaggtatc tgaaagcagg tgaataaata
     8101 catcttcaat ggtcttcata ttcatatttt tctccagtga aatcactgcg agccatacgg
     8161 cccgcagatg caattatctt tatcagctga tattaataat taacggtgaa tcaggattta
     8221 cctgatttat taccaccgct ttgttgaccg ccttttttac ccgcttcaga tgcgcgttgc
     8281 ggatcatttt taaaattacc gccgctatgc tgaccgcctt tacggcctgc gtcggatgcc
     8341 ttctcacggt cttcggcgaa atttcctgaa ccaccacgat gttcggccat gttatttctc
     8401 ccgttgcgtt gcattgtttc attaatatga gtgttgtgtg tcgacactca ttaaaattag
     8461 tcgctaatga gaattagtca aattaagcgc aacgagaaga tagagggaaa atatattttg
     8521 aggaacattc tggatatatt aacaattacc tgaggaataa gtgactta
//
LOCUS       DAZFRN010000185         8419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFRN010000185 DAZFRN010000000
VERSION     DAZFRN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.187"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            108..2486
                     /gene="ppsA"
                     /locus_tag="ACHKUB_004373"
     CDS             108..2486
                     /gene="ppsA"
                     /locus_tag="ACHKUB_004373"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO6657306.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2543..4189)
                     /gene="fadK"
                     /locus_tag="ACHKUB_004374"
     CDS             complement(2543..4189)
                     /gene="fadK"
                     /locus_tag="ACHKUB_004374"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO6657307.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4246..4539)
                     /locus_tag="ACHKUB_004375"
     CDS             complement(4246..4539)
                     /locus_tag="ACHKUB_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6657308.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4536..5825)
                     /locus_tag="ACHKUB_004376"
     CDS             complement(4536..5825)
                     /locus_tag="ACHKUB_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6657309.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5881..6819)
                     /locus_tag="ACHKUB_004377"
     CDS             complement(5881..6819)
                     /locus_tag="ACHKUB_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO6657310.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VTINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6839..7603)
                     /locus_tag="ACHKUB_004378"
     CDS             complement(6839..7603)
                     /locus_tag="ACHKUB_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO6657311.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7919..>8419
                     /locus_tag="ACHKUB_004379"
     CDS             7919..>8419
                     /locus_tag="ACHKUB_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO6657312.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         2006 a         2178 c         2178 g         2057 t
ORIGIN      
        1 tatttgcttg aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc
       61 agaaatgtgt ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg
      121 gctcgtcacc gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg
      181 ggggcaaaaa tgcctccctg ggtgaaatga ttactaacct ttccggaatg ggtgtttccg
      241 ttccgaatgg tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg
      301 taaaccagcg catttatgaa ctgctggata aaacggatat tgacgatgtt acccagcttg
      361 cgaaagcggg cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg
      421 aaaacgccat ccgcgaagct tatgcacagc tttccgccga tgacgaaaac gcctcttttg
      481 cggtgcgctc ctccgctact gcagaagata tgccggacgc ttcctttgcc ggtcagcagg
      541 aaaccttcct caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa catgtatttg
      601 cttctctgtt taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg
      661 gcgtggcgct ctccgccggt gttcaacgga tggtgcgctc tgacctcgca tcttctggcg
      721 tgatgttctc cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat
      781 ggggccttgg tgaaatggtc gtgcagggcg cggttaaccc ggatgagttt tacgtccata
      841 aaccgacact tgcagcgaat cgcccggcta tcgtgcgtcg caccatgggg tcgaaaaaaa
      901 tccgcatggt ttacgcgccg acccaggagc acggcaagca ggttaaaatc gaagacgtac
      961 cgcaggaaca gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac
     1021 aggccgtaca aattgagaaa cactacggtc gcccgatgga tattgagtgg gcgaaagatg
     1081 gccacaccgg caaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc
     1141 aggtcatgga gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta
     1201 tcggtcatcg catcggcgcg ggtccggtga aagtcatcca tgacatcagc gaaatgaacc
     1261 gcatcgaacc tggcgacgtg ctggtcactg acatgaccga cccggactgg gaaccgatca
     1321 tgaagaaagc atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca
     1381 tcgctcgtga actgggcatt ccggcggtag tgggctgtgg tgatgcaaca gaacggatga
     1441 aagacggtga gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg
     1501 agttgctgga atttagcgtg aaaagctcca gcgtagaaac gatgccggat ctgccgttga
     1561 aagtgatgat gaacgtcggt aacccggacc gtgctttcga cttcgcctgc ctgccgaacg
     1621 aaggcgtggg ccttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac
     1681 gcgcactgct tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga
     1741 tgaaaggttt tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga
     1801 cgctgggtgc cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga
     1861 acgaatatgc caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc
     1921 tcggcttccg tggcgcgggc cgctatgttt ccgacagctt ccgcgactgc ttcgcgctgg
     1981 agtgtgaagc agtgaaacga gtgcgcaacg acatggggct gaccaacgtt gagatcatga
     2041 tcccgttcgt gcgtaccgta gatcaggcga aagcggtggt tgaagaactg gcgcgtcagg
     2101 ggctgaaacg tggcgagaac gggctgaaaa tcatcatgat gtgtgaaatt ccgtccaacg
     2161 ccttgctggc agagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgata
     2221 tgacacagct ggcgctcggt ctggatcgtg actccggcgt ggtgtctgaa ttgttcgatg
     2281 agcgcaacga tgcggtgaaa gcattgctgt cgatggctat ccgtgccgcg aagaaacagg
     2341 gcaaatatgt cgggatttgc ggtcagggtc cgtccgacca tgaagacttt gccgcatggt
     2401 tgatggaaga ggggatcgat agcctgtctc tgaacccgga caccgtggtg caaacctggt
     2461 taagcctggc tgaactgaag aaataaaata aatccccggc ggcgttcagt cgccgggttt
     2521 atgtgatccc cgaagataaa acttattcaa tctcttcaca gacatcctgc gttaatcgcc
     2581 gcataatatc ttttcttaac aaaaactttt gtattttacc tgaggcagtg cgcggtagtt
     2641 tttcgattac cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa
     2701 aagccaccac ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc
     2761 atgatcgttc acctaaacgt tcatcgggca ttgcaaccac acaggcatcg tgaattttag
     2821 gatgctgcaa taaaatatct tccacttcac ggctgctaat attttcgccg ccgcggacaa
     2881 taatatcttt tttgcgtccg gtaattttta tatagccagc ctcatccata cggcagagat
     2941 cgccgctgta ataccagcct tcttcatcca gcgcacgggc ggttaattca ggttcatcaa
     3001 aatatcccat aaacacattg gggccacgcg aggcttcttc accttcgcaa cctggcggta
     3061 aggtcttgcg tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg
     3121 tgtgcataaa gcgcgacaaa ggatcatcga gattcaccac cgcatgcggc gaactttctg
     3181 tggaaccata aacacttaat aatttaatgc cgcgctgctg gcattcacgt gccacttttt
     3241 tggggattgt ggtaccgccg caaagaaaga aacgcagcgc tgaaaggtcc gcgggttgtt
     3301 tctctagtac attcaaaaga tcatagacaa acggcgttgc gcctagcata caggtgcaac
     3361 gctgctgctc aagcagcgcg agacacgcat caggagtgaa aatatctaac aacacgctgc
     3421 gagcgccaat taagaatggt gccgttacgc catgcagaaa gcccgttgcg tgaccaagtg
     3481 gcgcaggcat cataaagaca tcctgccagg tcagattcag tcgcgcgcaa taagcccgct
     3541 cactggcgag aatattgtta tgcgttagca tcacgccctt tggcagaccc tcggttccgg
     3601 aggtaaacag caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg gtcagtgagg
     3661 tattgtcggc gataatctga cttaatgaga gggaagaggt ggcgggagcc agtttgtcca
     3721 caccgacaat ttgttgtagt tgtggaagct gattttgcag cggcaggatt aaatctaccg
     3781 gacgcgtttg tttaaacaac gtcggtgcaa agaacatttt tgcctgacac ttattgagca
     3841 cccacaccag ttctgcttcc cgccaggaag gcaacagcgg cacggaaacc gcaccggttt
     3901 tcaggcaggc aagatagata acggtaaatt cacaccagcc tggcagttga aatgcgatgc
     3961 gatcgcctga ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat
     4021 cgagcgcgct ataggtgtac gatgctccat gattatcgac cacggcaatt ttgtctggca
     4081 tcgcacgagc ggtctgctgc cagtaatcgg ccagcgaagc atcgccccat agcccttgct
     4141 gacgatacgc cgcacgacgt tgttcgttga acgttaatgt cactttcatg ttggactctc
     4201 gaaacagaac atcaggcccg agatgcgggc ctgtgggatg catgattagc cgtagcgaaa
     4261 atcaataccg aaggtgcctg cgggatattg ccactgttcg agaatagtgt taccgcacag
     4321 cacccgacag gtgccgcact ccagacaacc ggcagaatca aaatgaatgt tgccggcgtc
     4381 atcctgctta taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc
     4441 gggatttgcc gccagaatga tatgcgggtg gccctcatca acatggaatt tattgacgcc
     4501 taatttgatg tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg
     4561 ccatctttca gcaagttgat cagcccaatt ttcttcgcat gtcccatgat cattttgcgt
     4621 accggctggt ttggtttgcc gtcaatggtg aacatctcgt tcatgatgtc ggcgaccatt
     4681 cgtgggtatt ggctaaacag gcgcgggttt tccatcagcg ccgggatctt gcggaaatgc
     4741 tgcatatcgc gcatgacgca gctttgttcc agctcacgtt tgtattgcgc cagactgctg
     4801 gcggagaaat cctcgcgttc tttggcggcg atcaccgttg tggcggcagc ctgagccgat
     4861 gcaatggcta aatccatgcc acgaactgta aaacccaaat tcaggcagaa gcctgcggcg
     4921 tcaccaacga tcatcacgcc atcgttaacc agctgcggca ccattgccag accgccttct
     4981 ggcaccatat gcgcggaata ttcaagcagt ttgccgccgc taattagtgg gcgaatggcg
     5041 gggtgttgtt taaaatcttc cagcatttgc ggcacgcttt tttgcgcatg ggcgatatca
     5101 cccaatccac aaaccagccc caaggatatg gaatccttgt tggtatagag gaatccgcca
     5161 cccatcaggc cgtcagaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta
     5221 atattaaagc gatcgttgat ctgttctggc gtgaggccaa taacctcttt aacaccaaca
     5281 gcgtaatgat gcggatcgga agcgggaacc attcccagcg agcggccaag catcgagttt
     5341 acgccatcag ctagaaccac cacattcgct tggagaatat catccccggc ctgcacgcca
     5401 gtgaccttgt ttccttcacg aaccaacgca tcgacgcgaa cgcccgggat aaactgtgcg
     5461 cccgcctgct cggcttgttc catcaaccac gggtccagac gattacgcaa tacggtataa
     5521 gatgcgtgtt gcggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctttct
     5581 tcggttaaga aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatactgca
     5641 aagcctggaa tgattgcttc aagtgtgtgg gcataaagac gcccgccggt catgttttta
     5701 catccggcac tgtcgccgcg ttctatcacc aggacatcca gcccggctcg cgccatgacc
     5761 agtgcggcaa cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa tttgtcttcc
     5821 gacatgcgtt atgctccctt cccgctgcgt ttacagcggg agatgtggtt gaaaggggaa
     5881 ttagcggctc aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc
     5941 gtagtcggca taattgaaga ttggcgcatt tttatcttta ttgatggtga caatcacttt
     6001 tgcgccgttg ccgccaacca tatgctggat ctgcccggag atccccagcg tcaggtagag
     6061 atcggatttc agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc
     6121 gccttcggca attggacgtg aacagccgac ttcagcattc agcaccgccg ccagttcgtg
     6181 gaccattttc aggtcatcct gcgccgccag accacgaccg acgccaacca cacgttttgc
     6241 tttgctcagg tccacgctgc ttgcggcttt cgcacggcgt tcgcgacaga gaatttcatg
     6301 acgcggagca acataaggta ccgtttctgt cgggcactga tgagaggtat cactggtgca
     6361 cggttcctga acaccgggtg caagggtaat gatcgccagc gggctgttga tcttttcctg
     6421 agcgaacgct aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc
     6481 caccgccgtg gcatcgttca ccagcgccgc attcagttgc acacttaacc gtgctgccag
     6541 agctttacca cgtttcgtcg cggccaacag caacatagcg gggtgtttat ctttcagcag
     6601 ggcagcaatg ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt gctcaagaac
     6661 ataaatacat tttggaccat aaggcataac tgcctgcgcc tggtcggtat tttgtacaat
     6721 ggcatacacc tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg
     6781 attatcgcta aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc
     6841 agttcagggc tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat
     6901 cgataatgat gtgcttacgt tctgtttgcg gcggtacgcg aatgccgacc agttcagcaa
     6961 gtggcgcgct ctggctccag tcaatatcac ttgcctgcca ctgatttacc ggttttttac
     7021 ccgcgccgag aatggctttc atcgaaggaa tgcgtggcac gttaatatcg gaggtgacgc
     7081 agagcacggc tggaacagag agttcaataa cttcaacatc atcttcaagc gtgcgttcaa
     7141 tcaccagtgt attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca
     7201 gaatttctcc gaccagcaag ccaacctgct gggcataaag gtcgccggaa ccttcaccaa
     7261 agatcagtaa atcgaagccg atcttttcaa ttgctgccgc cagcgctttt gcggtatcga
     7321 gaggcagtgc atgttcaagt tgcgcatcct gcaccaaata caggctgtgc ggcccgcggg
     7381 atagcacgtc tttgcgcact ttcgagttct gcaacaatga gccgccaacg gtcagcgcgg
     7441 ctatctcatc gtcatctgtt gcgagctggc ttgcagcttc aatggcattg agatcgaact
     7501 ggctgatttt ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgt
     7561 cctgttcttc aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc
     7621 gcatacggaa ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg
     7681 tgcggggttt tcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg
     7741 atcgctaaaa caaaagtgca atttgcggca atatagttat tccatgaccg tacaatcttg
     7801 cattcagtgt aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca
     7861 cgccgtattt tatccggcat atattgtttt tgcttgctca tggaataggg aggttgtcat
     7921 gtatcaacgc tgttttgata acgccagtga aacgctgttt gtcgccggta aaacgccacg
     7981 gctttcacgt tttgcattta gcgatgatcc aaaatgggag tccggacatc acgttcatga
     8041 caatgaaacc gagttgattt acgtcaagaa aggggttgca aggtttacca tcgattcttc
     8101 gttatatgtc gcacatgcgg atgacattgt ggtgatagaa cgcggcaggc tacatgcggt
     8161 ggcctctgac gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt
     8221 tcagggggct gaggaaaatc agctactgca accgcattct tgtccggtaa ttgccgcggg
     8281 gcagggaaaa gaagtcataa aaaccttatt taatgagcta agtgtgattt tgccgcaaag
     8341 taaaaatagc caaacatctt cgttatggga tgcatttgcc tatacgttag ccattcttta
     8401 ctacgaaaat tttaaaaat
//
LOCUS       DAZFRN010000186         8387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFRN010000186 DAZFRN010000000
VERSION     DAZFRN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.188"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            333..1673
                     /gene="dcuB"
                     /locus_tag="ACHKUB_004380"
     CDS             333..1673
                     /gene="dcuB"
                     /locus_tag="ACHKUB_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO6657313.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1751..3397
                     /gene="fumB"
                     /locus_tag="ACHKUB_004381"
     CDS             1751..3397
                     /gene="fumB"
                     /locus_tag="ACHKUB_004381"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6657314.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3520..4149
                     /locus_tag="ACHKUB_004382"
     CDS             3520..4149
                     /locus_tag="ACHKUB_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO6657315.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4288..5709)
                     /gene="melB"
                     /locus_tag="ACHKUB_004383"
     CDS             complement(4288..5709)
                     /gene="melB"
                     /locus_tag="ACHKUB_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO6657316.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5812..7167)
                     /gene="melA"
                     /locus_tag="ACHKUB_004384"
     CDS             complement(5812..7167)
                     /gene="melA"
                     /locus_tag="ACHKUB_004384"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO6657317.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7450..8358
                     /gene="melR"
                     /locus_tag="ACHKUB_004385"
     CDS             7450..8358
                     /gene="melR"
                     /locus_tag="ACHKUB_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO6657318.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2153 a         2216 c         2049 g         1969 t
ORIGIN      
        1 agattaatct ttaaacctaa tgatatctgc cctgagaaca aaaaatagac cgataaatat
       61 caataagata acagcaaaca aaacattaac atctgcgcag tacaaactat aaacccatcg
      121 ccagagagtt tttctctctg aaaaagccgc ttatcacagt gcataaattt gccgctgctt
      181 taatcagcca atatttactg tgaggtattt gctaaagccg gtaacgacca aacggatatt
      241 tagtcaggct ctgaaaacag ttcatacaaa acagaacgtg actgtgatct attcagcaaa
      301 aatttaaata ggattatcgc gagggttcac acatgttatt tactatccaa cttatcataa
      361 tactgatatg tctgttttat ggtgccagaa agggtggtat cgcgctgggt ttattaggcg
      421 gtatcggtct ggtcattctg gtcttcgtct tccaccttca gccaggtaaa ccaccagttg
      481 atgtcatgct ggttatcatt gcggtggtgg cggcatcggc gaccttgcaa gcttcgggcg
      541 gtcttgatgt catgctgcaa attgccgaga agttgctgcg ccgcaacccg aaatatgtct
      601 caattgtcgc gccgtttgtg acctgtacac tgaccattct ttgcggtacg ggtcatgtgg
      661 tttacaccat tctgccgatc atctacgacg tcgccattaa gaacaacatc cgtccggaac
      721 gtccgatggc ggcaagttct atcggtgcac agatggggat tatcgccagt ccggtgtcgg
      781 ttgcggtcgt gtctctggtt gcaatgctgg gtaatgtcac ctttgatggt cgccatcttg
      841 agttcctcga cctgctggca atcaccattc catcgacgtt aatcggtatc ctggcgatcg
      901 gtatcttcag ctggttccgc ggtaaagatc tggataaaga cgaagagttc cagaaattca
      961 tctccgtacc ggaaaaccgt gagtatgttt acggtgatac cgcgacgctg ctcgataaaa
     1021 aactgccgaa aagcaactgg ctggcaatgt ggattttcct cggtgcaatc gctgtagtcg
     1081 cccttcttgg tgctgattcg gacctgcgtc catccttcgg cggcaaaccg ctgtcgatgg
     1141 tactggttat tcagatgttt atgctgctga ccggggcgct gattattatc ctgaccaaaa
     1201 ccaatcccgc gtctatctca aaaaacgaag tcttccgttc cggtatgatc gccatcgtgg
     1261 cggtgtacgg tatcgcatgg atggcagaaa ccatgttcgg tgcgcatatg tctgaaattc
     1321 agggcgtact gggtgaaatg gtgaaagagt atccgtgggc ctatgccatt gttctgctgc
     1381 tggtttccaa gtttgtaaac tctcaggctg cggcgctggc ggcgattgtt ccggtcgcgc
     1441 tggcgatcgg cgttgatccg gcatacatcg tggcttcagc accggcttgc tacggttatt
     1501 acatcctgcc gacttatccg agcgatctgg cagcgattca gtttgaccgt tccggcacca
     1561 cccacatcgg tcgcttcgtc atcaaccaca gctttattct gccggggttg attggtgtga
     1621 gcgtatcgtg cgtcttcggc tggatcttcg ccgcgatgta cgggttctta taaatgcact
     1681 ttgcgtgccg cccgtgacta cgcggcacgc cattttcgaa taacaaatac agagttacag
     1741 gctggaagct atgtcaaaca aaccctttat ctaccaggca cctttcccga tggggaaaga
     1801 caataccgaa tactatctac tcacttccga ttacgttagc gttgccgact tcgacggcga
     1861 aaccatcctg aaagtggaac cagaagccct gaccctgctg gcgcagcaag cctttcacga
     1921 cgcttctttt atgctccgcc cggcacacca gaaacaggtt gcggctattc ttcacgatcc
     1981 agaagccagc gaaaacgaca agtacgtggc gctgcaattc ttaagaaact ccgaaatcgc
     2041 cgccaaaggc gtgctgccga cctgccagga taccggcacc gcgatcatcg tcggtaaaaa
     2101 aggccagcgc gtgtggaccg gcggcggtga tgaagaagcg ctgtcgaaag gcgtctataa
     2161 cacctatatc gaagataacc tgcgctattc acagaatgcg gcgctggaca tgtacaaaga
     2221 ggtcaacacc ggcactaacc tgcctgcgca aatcgacctg tacgcggtag atggcgatga
     2281 gtacaaattc ctttgcgttg cgaaaggcgg cggctctgcc aacaaaacgt atctctacca
     2341 ggaaaccaaa gccctgctga ctcccggcaa actgaaaaac ttcctcgtcg agaaaatgcg
     2401 taccctcggt actgcagcct gcccgccgta ccatatcgcg tttgtgattg gcggtacgtc
     2461 tgcggaaacc aacctgaaaa ccgtcaagtt agcaagcgct cactattacg atgaactgcc
     2521 gacggaaggg aacgaacatg gtcaggcgtt ccgcgatgtc cagctggaac aggaactgct
     2581 ggaagaggcc cagaaactcg gtcttggcgc gcagtttggc ggtaaatact tcgcgcacga
     2641 cattcgcgtt atccgtctgc cacgtcacgg cgcatcctgc ccggtcggca tgggcgtctc
     2701 ctgctccgct gaccgtaaca ttaaagcgaa aatcaaccgc gaaggtatct ggatcgaaaa
     2761 actggaacac aacccaggcc agtacattcc acaagaactg cgccaggccg gtgaaggcga
     2821 agcggtgaaa gttgacctta accgcccgat gaaagagatc ctcgcccagc tttcgcaata
     2881 cccggtatcc actcgtttgt cgctcaccgg caccattatc gtgggccgag atattgcaca
     2941 cgccaagctg aaagagctga ttgacgccgg taaagaactt ccgcagtaca tcaaagatca
     3001 cccgatctac tacgcgggtc cggcgaaaac ccctgccggt tatccatcag gttcacttgg
     3061 cccaaccacc gcaggccgta tggactccta cgtggatctg ctgcaatccc acggcggcag
     3121 catgatcatg ctggcgaaag gtaaccgcag tcagcaggtt accgacgcgt gtcataaaca
     3181 cggcggcttc tacctcggta gcatcggcgg tccggcggcg gtactggcgc agcagagcat
     3241 caagcatctg gaatgcgtcg cttatccgga gctgggtatg gaagctatct ggaaaatcga
     3301 agtagaagat ttcccggcgt ttatcctggt cgatgacaaa ggtaacgact tcttccagca
     3361 aatcgtcaac aaacagtgcg cgaactgcac taagtaacct cttcggccca gcgcctggca
     3421 gcatgctgcc aggtgatccc cctgggccac ctcttttgcg attgtaattt cacgcttgct
     3481 ggtgaatagt cagtattttc cctgacttgc gaactcacca tgacccgcac actcaagccg
     3541 ttaattctta acaccggcgc actggcgcta acattaatcc tgatttatac cggcatttcg
     3601 gcccatgaca aactcacctg gctgatggaa gtgacaccgg tgattattgt cgtgccgcta
     3661 ctgcttgcca ccgccagacg ttatccgtta acgccgctgc tctatacgct cattttcttt
     3721 cacgccatca tcctgatggt cggcgggcaa tacacctacg cgaaagtccc cgttggtttt
     3781 gaggtgcagg aatggctggg gttgagtcgt aatccgtatg acaagctggg gcactttttc
     3841 caggggctgg tacctgcact ggtggcacga gaaattctcg ttcgcgggat gtacgtgcgc
     3901 ggacgtaaaa tggtggcgtt cctggtctgc tgcgtggcgc tggcgataag cgcgatgtat
     3961 gaattaatcg agtggtgggc agcgctggca atgggtcagg gagcggatga ttttctcggc
     4021 acccagggcg accagtggga cacgcaatcc gatatgttct gcgcgctgct tggcgcatta
     4081 acgacggtga tattcctcgc tcgctttcat tgccgccagc tacggcgctt tggcttgata
     4141 accggataac aacttgcgct ttgttgatgc cggatgcggc gtgaattcct tatccggtct
     4201 acaaaatttg caaatccaaa gcattacatt aagacgtggc ctgataaaca tatcgcatca
     4261 ggccattttc gttagctatt gtccacgtca ggctttcaca tcactcactg caccgaccgg
     4321 aatatcagca tgaacaggct cgggcggtac tttgcgatat ttatccagca gatgaatctg
     4381 gatcctgcgc agcgtgtcgc cattgaggcg atagaaacgg aagtagagaa tcagcgttac
     4441 catgaaaaac agagttggta gagcaatcat aataaactgc atacctaata gggcttgcgt
     4501 agactgttca acattcggta catagccaat catccctaac acaaccgcaa tgaaaaaagc
     4561 cgcaaaggct gaaccgccct tcaccaccat agtctgcacg gagtaagcga tactttcaca
     4621 gcgtacgtgc agtttatatt caccgtaatc aacggtatct gccaccatga tgacctgtaa
     4681 tacccagaaa agcgccgttc ccacattcag caaaataccc gcaatcacaa tgaggacgac
     4741 gttgtgatag ctcattaatg ccattaacag gagaacacca cagcttaaca ccggaagaat
     4801 agatgctccg gcccataaaa tgcgtcggga taatgattta actaagcgtg ggaagaatac
     4861 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
     4921 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
     4981 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
     5041 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
     5101 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
     5161 agtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
     5221 cgtcacacct gccgtaacaa agcctgccag actggcaaaa aaacgcggat aaggaaccag
     5281 ttgttcgcgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
     5341 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
     5401 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
     5461 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
     5521 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
     5581 caaacccaca gacagcccga cgacatcggt gtagtaatac atgaggtaca tatacacaat
     5641 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
     5701 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
     5761 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
     5821 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     5881 ttcatcaatg cccagcacgg cagcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     5941 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacgttga tgttggtttg
     6001 catcagggcg gccagatgcg aaggtaacgt accgactttg gtcggctgaa tgccattagc
     6061 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     6121 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     6181 gctggcatat tcccgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     6241 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     6301 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggtg tgtactcagc
     6361 aaaatgttct gacgattccg tgacgaagta gcccagcttt ttgaacattt cgtagcgcac
     6421 aatattctgg cagcgagtat tgccatgaat attcggcttc ggtgcctgcc ctgcttcata
     6481 agccgccagc agttccgggt agagattcac ataactgccg tcggcggttt tgcgctccag
     6541 ctccaggtaa aacgccatat ggttgatacc tgcgcaacgg taacgcagcg tagctgggtc
     6601 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagcccgac
     6661 ctgtttgata tgcggatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     6721 atagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     6781 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     6841 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     6901 gccaatctga aatgccacca cgacaaaatc ggcatcctct aaggcttctt tctgttgggt
     6961 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     7021 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     7081 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     7141 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     7201 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     7261 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     7321 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     7381 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     7441 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     7501 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     7561 atgcccacca gccactggca tggtcaggtc gaagtgaatg tgcctttcga tggcgatgtg
     7621 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtcatatcac gctgttctgg
     7681 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     7741 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     7801 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     7861 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     7921 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     7981 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     8041 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     8101 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     8161 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     8221 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     8281 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     8341 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaata
//
LOCUS       DAZFRN010000187         8374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFRN010000187 DAZFRN010000000
VERSION     DAZFRN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.189"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(1..720)
                     /locus_tag="ACHKUB_004386"
                     /pseudo
     CDS             complement(1..720)
                     /locus_tag="ACHKUB_004386"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
     gene            complement(753..1670)
                     /locus_tag="ACHKUB_004387"
     CDS             complement(753..1670)
                     /locus_tag="ACHKUB_004387"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO6657319.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLAQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKKMKII"
     gene            1788..2340
                     /locus_tag="ACHKUB_004388"
                     /pseudo
     CDS             1788..2340
                     /locus_tag="ACHKUB_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2580..3266)
                     /locus_tag="ACHKUB_004389"
     CDS             complement(2580..3266)
                     /locus_tag="ACHKUB_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO6657320.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPRSVASMMAQMQLGNVKVLFESKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
     gene            complement(3378..4325)
                     /locus_tag="ACHKUB_004390"
     CDS             complement(3378..4325)
                     /locus_tag="ACHKUB_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6657321.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLVGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAKWPGRETLPQPRLYLVEECTEEQTKASPLPVPLVSAQ"
     gene            complement(4439..5416)
                     /locus_tag="ACHKUB_004391"
     CDS             complement(4439..5416)
                     /locus_tag="ACHKUB_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO6657322.1"
                     /translation="MKTKKHTSRTAIKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSADALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRKSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            complement(5509..5892)
                     /locus_tag="ACHKUB_004392"
     CDS             complement(5509..5892)
                     /locus_tag="ACHKUB_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657323.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            complement(5995..6636)
                     /locus_tag="ACHKUB_004393"
     CDS             complement(5995..6636)
                     /locus_tag="ACHKUB_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657324.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTLVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRLLPVGGNRTP"
     gene            complement(6718..7095)
                     /locus_tag="ACHKUB_004394"
     CDS             complement(6718..7095)
                     /locus_tag="ACHKUB_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657325.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            complement(7291..7770)
                     /locus_tag="ACHKUB_004395"
     CDS             complement(7291..7770)
                     /locus_tag="ACHKUB_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657326.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(7853..8245)
                     /locus_tag="ACHKUB_004396"
     CDS             complement(7853..8245)
                     /locus_tag="ACHKUB_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657327.1"
                     /translation="MSTFILYPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
BASE COUNT         2253 a         2328 c         1791 g         2002 t
ORIGIN      
        1 tccgacaaca acaacctgac aatcaagcag taattttaaa cttactatta attcagcaat
       61 agtactggca gaacgctctg tcaaaaattc ggctttctta tatccctgtc tcgctaattc
      121 aaaaatatat ttggtgctat atcctgcaag ttcatttttt gctgccccca tgatggcccg
      181 accagatgaa accgcttcaa cgcatccccg acgaccacat ccacattctg tatccgttac
      241 tccggacaat atatgcccaa catgtccggc taatccagaa acacctgtta ataattttct
      301 gttaactaca attccccctc caacccccgt agaaacggtt ataaaaacca tatcacaaat
      361 ttcttttggc aaaactgtat attctgccca tgcggctgcc taggcatcat ttatcacact
      421 tccatttaaa ccagttatat cttccattat atttttcaga ggatactctt tcagccctcc
      481 aagattgtct ggattcaaag ctgtcaatac tccattatta ataatccctg ttgatgctac
      541 tgcaaatcgc tccgctgttc ccttcatttc agaaaccagt agacgtaaag cccctgtcat
      601 ttcgtcagga catctttcat gaggtgttga tatctgtttt ttcagcaaaa atgaaccgtc
      661 atcagatatc aacgccgctg aaattttcgt tcctcctata tcaacagcca gtgttatcat
      721 tcattttacc tatataacaa tcttgcaatt attcagatta ttttcatttt tttatttctg
      781 tcatgttctt cgagcagtcg ctctgcaagt tttgataata catccagatt cttttccttt
      841 accggagaaa aaggacttct gcatacagga gtatttataa tccccatata gtaaagaaga
      901 gtttttatac ctgccaggac accactctga ataagagtat caataacctg gttacaggcc
      961 acctgcattt ccttcgcctt aatcacatca ttatttttga ctgcttcaaa tatttcaaga
     1021 taacgccacc ccattatatt atatgtacta ccgattcccc catctgcacc tgcaattaac
     1081 cctgatgcaa atatttcatc gtaaccatta tataacacaa gctctggatg attccttttg
     1141 atttgttcca tctggaataa atcaccggat gtctgtttta atgcacaaac cccgggtatt
     1201 gtgacaagct catttatctg agctaaagaa aaacgcactc cgctaagagc cggtatatta
     1261 taaactacca tcggaagtcc atctgcagaa tcaataatct tcctgtaatg gatacaatgc
     1321 tcttcaaatg aaaaaggata ataaaatgga gtaacagcag atatagcatg atatccgtat
     1381 ttttttgcag cttttgcaag tacctcgctt tctgccgtac tgattccgcc cacatgcgct
     1441 ataagtgtta gtcttccatc tgattcatcg gctactgttt caagaatttt ttctcgctct
     1501 gcaacattct gaagaaatgc ttctccagta gagcctccaa catacaatcc cttaatattt
     1561 tgttcgatat taaaacggat cagttttcga agactctcca tatcgatttg ctgggattgg
     1621 tcataaggag tgggtaatgc tgatatgacc cctttaaatt cacattgcat ttttaatcac
     1681 cttcacttaa ctgcataatg ggatacctag tacaccatca agtacaaaga ttatccaaca
     1741 gaaaagaagc acaatgtgaa gattatcaaa aaaaagccat cagaaagctg tcatgccgct
     1801 cagttaagga tcggttgatc gatccagtgt ccagttacta ttctctgcgt aaccctctgc
     1861 gactatcttg ccctggaact gattacccac agccctgaag agtccgacac tgtcacgcta
     1921 cacaaacggc gtcttcctct ggaaatgatg gtctggtgaa ttatcagtat ggcccatgag
     1981 cgtaaggaac cttcatcaga ttgtgaaccg gctggacatc atgctgccag gcagtagcgc
     2041 cgtgctccat gcccgccagc ggctaggaag cggagccata cgctgcgtgt tcactcaaac
     2101 agcgcagctc tagcataaca ccacgccata tccgctctgg tgcgatctga atctgctgac
     2161 cattgatggc gtgttctgac gaacacagga cacgcaagaa cacgataaag cttcactcgc
     2221 caaatacatg gtggaaacca gacactttat cctcaggtaa aaatggtctg tctgatagaa
     2281 ctgaccagcc atctactgac ggcagcggcc ttcgatacaa tgaatctggc actaaataag
     2341 actggcaata tagccgataa tgcgttgaat cagtggctat atcgtataca taaaacagac
     2401 atcattattc tgtcttgttg tccaacacat aaaaactaat aaaaacatac cattccctgt
     2461 cgtcagagaa tttattgata aacaggcttt attgaacaaa tccaaatcaa acattcaggg
     2521 aactggcagt tgaacaaaac gccagtccga agactggcgc agtgataaaa cattctgcat
     2581 catgccacat tctgctcctg aagatgacgc agacgatggt cccagtttcc caggtaatgc
     2641 ccgggagtca gcagtacgcg gcgtcgctca ttacagagcg agttaccaat caccacttct
     2701 ccggccacac cacacagcga cagttgtata tacgccatgc cggctgccag cgggtcaata
     2761 tccgttgccg acacccacat cctgtggtat gccggatacc ccgaacggtt caggatgtca
     2821 gccattgcca gaatcatact gccggcacca caggcaggtt cactgagcgt gataaagggt
     2881 ttactctcaa acaacacctt cacatttccc agttgcatct gtgccatcat gctggcaaca
     2941 ctcctgggcg tgaaaaactg actccggtat ttatccccca gttccagttg catgaacaca
     3001 ctacccagaa aatcacagaa tccctgctcc agtccctcaa tgacatgagc aagcaaatgc
     3061 gcaatgtgga taacatcctg cttttcataa ccactgacga ttcgcagata cttctgttcc
     3121 cgggcctcac tgaactgaag ccggttttcc agggcaatca cactacagct gacaaagtct
     3181 tcaaacacct tatgacgttt gtgataacag gctgtccggt taaacaacga aatgaattcc
     3241 ttctgatggt cagtcatggt ggccatgttt tctccgtaaa aacagggaac acatggcccg
     3301 gacaggggaa caggtgttcc ccttcgggtt gaaatgttga taaaacgggc atcacggaca
     3361 tgatacccgg aggttaatta ctgggctgag accagaggaa ccggtaaagg tgaggctttc
     3421 gtctgttctt cagtgcactc ctccaccaga tacagtcggg gctgtggaag tgtttccctg
     3481 cccggccatt tggcctgcag atcactgtcc gtatgctctc cgccatcgta acagttgtaa
     3541 ccctgtatac cgtgtaccgg ctcgctgtac cagtgtcgga cctcgcagtc aaacaccttc
     3601 gagatctcac caaccagttc gcctgaaggc ggataccagg gcgtgtcgat cagcagcacc
     3661 agactgctca tctcatcacg cgcatggcga acattatttc ctgacggcca ctccacaccg
     3721 tatatccggc tgtaaaggct ggtcgttgtg gaaataccct gcagcagtcc acctgagcca
     3781 ttcagctctg tagccagacg ggagggtatg accatcagca tgtcacaggg ctgcgcctgt
     3841 tccgggtaat ccgacaacca gctccagcag gcttctgcat cgatgtgttc actcagcccg
     3901 accagaccaa accagtcaga atactgacgg ctcatcagcg tggtgatgat ctccctggca
     3961 ttatccggca gattacccca gcgaacagcg ccgatacatg actggtgata aagacgctca
     4021 atggtcttta tcgtttcacc atccagaacg gcatttttta ccagcaaccc cagccactgc
     4081 tcaaacgcct ggttagccgg agatgacaat cccgttccgt gacgaaccag cgccggatat
     4141 ggcggatact gcgtcgtccg tgtgggtttt accagccctg cacaaccggc cagaaacagc
     4201 tgtatactct gctgtatggc atgacggtag cgcggggtct cacgcccttc aatccactgt
     4261 gtaagtacat caagacagac tgatttaccg gtaatttcaa aacggttttt gcaccattca
     4321 gacattgttt acgctcctct tttttataca gatgtaaaaa gagggcattg tcccgtgagg
     4381 gacaatgccc tcacgggata aatggcggtc aggttataac cgccaccggt agtaaaaatt
     4441 atgctgcgca ctcctgcgaa gttcgccgcg tcagtaaata ctctgatgct tcacgtgcct
     4501 gccggcaggc gcgaaacagc gcttttttgt cagacttcag aaccttaagc catccgtcaa
     4561 tataactttc gtgctgtacg tcaccggcaa tcccgagatg tgcacacaga aaagcactgc
     4621 ccatctcggc aatcagctct tcaaacgcat attccgggct tttgcgaccg gtaaaggtac
     4681 ttatccccac ccgccgcagg cgcttttcat gaccggtact gtgtaccagc tcatgcagca
     4741 gggtggtcca gtaatcagcc acctggtcaa actgcgacga cagtggcatc acgatacggt
     4801 caagatcagg ccggtagaat gccctgtcct gtggaagtga cgagacgctg acgccgctgc
     4861 cattcagtac ctgcagcacc cgctcactga aggtatctgc cggcagtgca tccgcgctgc
     4921 cttccgcctt caccatcaga tgttcaggta aaccgtcaca ctgccccaca ttaaacagag
     4981 gattcgattt cagtaccggt acgatttcca tgagaggctc cccctcactg tccgtcagcc
     5041 ggttaccgtg ttcatccaca gcctgttttt tccagtcttt gtaaatcacc gccagcgtgg
     5101 ccacctcccc tttacggacg tggccaccca gttgttgcgc ctgacggtac gtcagccagt
     5161 cattgctgac atacccggct tcatccgcgg ccagccacag caacaggacg ttcacccctg
     5221 tataaggctt cccggtcagg gcatttgccg gcatcatctc cggaagcccc acgccattac
     5281 gcgcagagcg ccagggacgt ttccagggtg gcacaccatt ttcaatggcc gtaacaaccc
     5341 tgtcggtcac aagctgatac aggtcacgtt ccggagtttt aatcgcggta cgtgaagtat
     5401 gttttttcgt tttcataaga gcatgccccc tgaaaaagat gcacgctccc tgtacggtga
     5461 gagtgcaccg tacagttaaa ttaataacga aaatcagact ggccagaatt accacaacgc
     5521 cagcatcacc agtgaacgac gctcaaaatc aaacgtcttc agcgtattgc tgacccacgc
     5581 ttttacgtca tccacgtcat accagtaatc accggcataa tcttcccccc gtaaacacga
     5641 gcctgtgaca taaggaaaca gcctgtggca gttccccggt gtcagcatac tgaggtcgcg
     5701 caataatgca gccagtgaac tgcgggttac agggaaagca tgctgctcat ctgaagcacc
     5761 ggtatgttca cgaaaccatg tcagaagcgg tttcacctta cggaaagccc ccacctcctg
     5821 atgttcctgt gttcccacaa cggcaccact gtgatggtta tcacgagcca gaaagtaaat
     5881 atcgagtccc ataacaattc tcctgaataa agccgggact ctccccacag ggatatgtcc
     5941 ctgccgggtg atataatgac ggaatacgtg atgacgttat tccggataaa gaaactacgg
     6001 cgttctgtta ccgcccaccg gcaagagccg cagccagtaa agctgatatt cggcctcttc
     6061 cagtgctgcc cagtcatccg gggataactg tcgcccctga ccggacaaaa actgcagcag
     6121 taagctgtca aacgcatctc cccttaccag ggtggttaat acagactccg gagcagaaaa
     6181 attctcatgc cccaggtacc gttttatcca tttcagcggc agcgaccagc tggcaccacc
     6241 gacagtggaa tgaatacgaa aacagtccgg acacagattg tgcaacagca caaagactgt
     6301 acgggcaaag aaatctcccg gctgacgtcc gaaattcagc tcattaagag ccaggatatt
     6361 ccggctgtgc tcccgctcaa tagtgacatt caccgaccac agcccgtcgg cagaccggca
     6421 gtaaaacgcc agagtgtccg ttaccggaaa caactctgac gcccgggtca gggagccgaa
     6481 atacgcattc tgcggccgta acgcctccgg caactgccag aaggtttcag tgacagccag
     6541 aacaaaagca tgccactgct tttccggtat tgtggaaacc tgctcaaggg tatataccac
     6601 cggctcacgg gccggacgtg aagataactc agacatggaa cattcctcat ggttaaaacg
     6661 aaacaacggg gaatgtctcc cggacggaga actcccccgt cagggtcagg tcatgaatta
     6721 catcatgtcg tgccgggcaa aatattcatc cgctccggcc accatctttc cggtactgat
     6781 actgtcagcc cagatttcat gattaatttc acgccccaga gccatcagac tgtcgagtgc
     6841 ctgacgggcc tgaattcccg gtgcggaaat ctcctcctgc tccaggtcat acaacagcca
     6901 gcccagcgcc tcgccggtat cccacagata cacgtatacc agtgaaggct ccgtctcatc
     6961 atccggctcc ggggtaatac ccagccgttg ccaggcacgt aaccgcatca gctcgccttc
     7021 catctgactg atatgagcca gcagagcaga ctgcgtctga agcgctgaat aatgagcaat
     7081 taaaggtgaa tacatgatga cctccgtctc ttaaaggaag tcctgtccct gacggaacaa
     7141 aactcccgtc agggaaaaca ccggcgcaaa aacacggtgc agtaagtaaa agaaaaaccg
     7201 ttgcgggagc aaaacagcca tcattgcaaa atggcagttg catcgctcac gatttgacat
     7261 ccttcctgtg actgactggc atcctgcccg tcagtctgcg tcggaaaatt caatacaggc
     7321 ctgcttcagg agcccggcta tacgttgatt cagacattcc gtccagccat cgttccatcc
     7381 acgctcccat ggattgcaga catcctccca tggattgcag acatcgttac agccattctt
     7441 ccatgtcttc catgcctcca tctccatgac agcccccagc ctgtggcctt tcacacgtat
     7501 atgccacaga aacggccgga aaagtcctga tttcagttgg tcaccaccgc ttttgtagcc
     7561 cagatgacgt gcaatacttt cagtatattc aaatgcatca ttctgcattc cagccgcctt
     7621 agccagcgag ctgtacgcat cacgaagtat cgtcatacct tccagttgat actcacacca
     7681 cgcacggaca gccgacactg gtgcgattac ccgaagaatg gtgcaggcac gccatacaac
     7741 aacgtaaagg agtattaaca gactatgcat gtgttcacct cagatacaaa aaataacggg
     7801 aacacacctg ccctgcggga agcgtgttcc cgacagggta ataaatgata aatcagcagg
     7861 gctgtgcgac gattccatca ccctgcagac caaaccggta acgcagggtg gtcagttgct
     7921 ccgccatacg gcaggcagca tgccatgtcc tgcgacggga tatccgtccg gtaaaccggt
     7981 tcagataaat gacctcacgc ggtaacggct gataccatcc tgtgtactcg ttgcgataca
     8041 ttccctgccg gtaatacagg cagggtgtcc agacaccgcc acgacggaac atacagaccc
     8101 gcacgctcag attgccggcc ggattagtgt accagcgact gtaatatgac aggttattgc
     8161 gggaaaaaaa cacccgcaac tgagccgtca ttcgccggaa aaaagaagag atgcccggtt
     8221 cagggtacaa aataaacgtt gacataaaga ctcctgtaaa aaaacgggag ccttcgcccc
     8281 gacggacaaa aactcccgtc agggttaaaa caccaccagt acagcatggt gttgctgtca
     8341 gaaaaagccc gttaataaag ggcgcagtta taaa
//
LOCUS       DAZFRN010000188         8353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFRN010000188 DAZFRN010000000
VERSION     DAZFRN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.190"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(82..288)
                     /locus_tag="ACHKUB_004397"
     CDS             complement(82..288)
                     /locus_tag="ACHKUB_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO6657328.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            601..690
                     /gene="kdpF"
                     /locus_tag="ACHKUB_004398"
     CDS             601..690
                     /gene="kdpF"
                     /locus_tag="ACHKUB_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO6657329.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            690..2363
                     /gene="kdpA"
                     /locus_tag="ACHKUB_004399"
     CDS             690..2363
                     /gene="kdpA"
                     /locus_tag="ACHKUB_004399"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO6657330.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2386..4434
                     /gene="kdpB"
                     /locus_tag="ACHKUB_004400"
     CDS             2386..4434
                     /gene="kdpB"
                     /locus_tag="ACHKUB_004400"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO6657331.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLRESKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRQMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4443..5015
                     /gene="kdpC"
                     /locus_tag="ACHKUB_004401"
     CDS             4443..5015
                     /gene="kdpC"
                     /locus_tag="ACHKUB_004401"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO6657332.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVKLNLALDKLDE"
     gene            5008..7692
                     /gene="kdpD"
                     /locus_tag="ACHKUB_004402"
     CDS             5008..7692
                     /gene="kdpD"
                     /locus_tag="ACHKUB_004402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO6657333.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7689..>8353
                     /gene="kdpE"
                     /locus_tag="ACHKUB_004403"
     CDS             7689..>8353
                     /gene="kdpE"
                     /locus_tag="ACHKUB_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO6657334.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYR"
BASE COUNT         1731 a         2180 c         2441 g         2001 t
ORIGIN      
        1 atattcctct gaacatcttc ctgatacaaa aaaccgccag cgtttccgct ggcggttttc
       61 aatgtattca aaactcagca atcaataaaa atcaccagtt gccttttcgg cctgatccat
      121 ccacaccggt ttatcgctgg ttttcgacca gacgcgatgc aggtagctgt aaaagcgggc
      181 gcggtctttc cagaaaagca tgaaaggcag cgccagaacg cccgctgcaa ccgcataagt
      241 acggcgtaaa aagataagcc atgcaggata ttctctgtag agttccatac ttttctcccc
      301 cttttgtatc tacccggtga atggcaccgg aaaaatgaat ttgtttatct gatgaaaata
      361 gtaccgcctt ttgtgtaatt ttactactca tccgaccact tatttttgct tattgatggt
      421 ttatttacat tcatcctgta attaagttac acaaaagtta aattaatact aaacattagt
      481 taaatcatgg cttttgccat ttttatactt tttttacacc ccgcccgcag atttttgcga
      541 aatctttgca gccagaattc tacccttccg gtatcacttt taggccactg gaggtgcact
      601 gtgagtgcag gcgtgataac cggcgtattg ctggtgtttt tattactggg ttatctggtt
      661 tatgccctga tcaatgcgga ggcgttctga tggctgcgca agggttctta ctgatcgcca
      721 cgtttttact ggtgttaatg gtgctggcgc gtcctttagg cagcgggctg gcgcggctga
      781 ttaatgacat tcctcttcca ggtacagcgg gcgttgaacg tatactcttt cgtttacctg
      841 gcgtctctga ccatgagatg aactggaagc aatatctttg tgccattctc ggcctgaaca
      901 tgctggggct ggcggtgctg ttttttatgt tgctcggtca gcactatctg ccgcttaatc
      961 cacagcagtt gccggggctg tcgtgggatc tggcgctgaa taccgccgtc agctttgtca
     1021 cgaataccaa ctggcaatct tacagcggtg aaaccacgtt gagctatttc agccagatgg
     1081 ctggcttaac ggtgcaaaac tttctttctg ccgccagcgg gattgcggtg atttttgccc
     1141 tcatccgtgc gtttacccgc cagagcatga gcacactcgg gaatgcctgg gtcgatctgc
     1201 tacgcatcac gttatgggtg ctcgtccctg tggcgttgtt gatcgcactg ttttttattc
     1261 aacaaggtgc gctgcaaaac tttcagcctt atcaggctgt gaataccgtt gaaggagcgc
     1321 aacagctgtt acccatgggg cctgtagctt ctcaggaagc gatcaagatg ctcggtacta
     1381 acggcggtgg cttctttaat gccaactcgt cgcatccgtt tgaaaaccca accgcactga
     1441 ccaacttcgt gcagatgctg gcgatcttct tgatcccaac ggcgctgtgc tttgcctttg
     1501 gtgaagtgac gggcgatcgc cgccaggggc gcatgttgct gtgggcgatg tcagtgattt
     1561 ttgtcatctg cgtaggcgtg gtgatgtggg cagaagttca gggtaatcct catctgctgg
     1621 cgctcggcgc agacagcagc atcaatatgg aaggtaaaga gagccgtttc ggcgtgctgg
     1681 tcagtagcct gtttgcggtc gtgactacgg cggcttcctg tggcgcggtg attgcgatgc
     1741 atgattcgtt taccgctctc ggtggcatgg tgccgatgtg gctgatgcaa attggtgaag
     1801 tggtgttcgg cggtgtcggt tctggtcttt acggcatgat gctgtttgtc ctgctggcgg
     1861 tgtttattgc cgggctgatg attggtcgta caccggaata tctgggtaaa aaaatcgacg
     1921 tacgcgagat gaaactgact gcactggcga ttctggttac cccgacgctg gtgctgatgg
     1981 gcgcggcgct ggcaatgatg accgacgccg gacgtagcgc catgctcaac cctggcccgc
     2041 atggttttag cgaagtgctg tacgccgtgt catccgccgc taacaacaac ggcagcgcct
     2101 ttgccggatt aagcgccaac tccccgttct ggaactgttt actggcgttc tgcatgtttg
     2161 tcggtcgctt cggggtgatt atcccggtga tggcaattgc cggttcgctg gtgagtaaaa
     2221 agagccaacc cgccagctcc ggcacgctgc caacgcacgg cccgctgttt gttggcctgt
     2281 taatcggcac cgtgttgctg gttggcgcac tgacctttat ccctgccctg gcgcttggtc
     2341 cggtggcgga atatctctcc tgatgatatt gagtgagcac tgaatatgag tcgtaaacaa
     2401 ctggcgctat tcgaaccaac acttgtcgtt caggcgctga aagaagcggt gaaaaaatta
     2461 aacccgcagg cgcaatggcg caatccggtg atgtttatcg tctggatcgg cagtctgctg
     2521 accacctgta ttagcatcgc gatggcaagc ggtgcgatgc ctggcaatgc gctgtttagc
     2581 gcggcaatta gcggctggct gtgggtcacc gtactgttcg ctaatttcgc cgaggcgctg
     2641 gcagaaggcc gcagtaaagc gcaggccaac agtcttaaag gggtgaaaaa aactgccttt
     2701 gcccgcaagc tgcgtgagtc gaaatatggc gctgcggcgg acaaagttcc tgccgatcaa
     2761 ctgcgtaaag gtgacatcgt gctggtagaa gctggcgata ttatcccctg cgatggtgaa
     2821 gtcattgaag gcggcgcatc ggtcgatgaa agcgccatca ccggggaatc ggcaccggtg
     2881 atccgtgaat ccggcggcga ttttgcctcc gtcaccggcg gcacgcgtat tctttctgac
     2941 tggctggtga ttgagtgtag cgttaacccc ggtgagacat ttctggatcg gatgatcgcg
     3001 atggtggaag gcgcacagcg acgcaaaaca ccgaacgaga ttgccctgac tatcctgctg
     3061 attgctctga ccatcgtctt tttactggca accgccacgc tgtggccgtt ttccgcgtgg
     3121 ggcggtaatg ccgtgagtgt aacggtactg gtggcgctgc tggtctgtct gatcccaacc
     3181 actattggcg gcctgttgtc agcgatcggc gtcgccggga tgagccggat gctaggcgca
     3241 aatgtgattg ccaccagcgg acgtgcagtt gaagcggcag gtgacgttga cgttctgcta
     3301 ctggataaaa ccggcaccat cacactcggt aaccgtcagg cgtcggagtt tatccccgcg
     3361 cagggcgtgg atgaaaaaac gctggctgac gccgcacaac tggcttcgct ggctgatgaa
     3421 acgccggaag gccgcagtat tgtgatcctc gccaagcagc gttttaacct gcgcgagcgc
     3481 gatgtgcagt cgctccatgc cacctttgta ccgtttactg cgcaaagccg gatgagcggg
     3541 atcaacatcg acaaccgcat gatccgtaaa ggttctgtcg atgccattcg tcgccatgtt
     3601 gaggctaacg gtggtcactt ccctaccgat gttgatcaaa aagtcgatca ggttgcgcgt
     3661 cagggagcca cgccgctggt ggtggtggaa ggttctcgtg tgctgggcgt tattgcgctg
     3721 aaagatatcg tcaaaggcgg tattaaagag cgcttcgcgc aactgcgcca aatgggcatt
     3781 aaaacggtga tgattaccgg cgataaccgt ctgactgccg ccgccattgc tgcggaagcg
     3841 ggcgtcgatg attttctcgc cgaagcgaca ccggaggcca agctggcatt gattcgtcag
     3901 tatcaggcgg aaggccgttt ggtggcgatg accggcgacg gcaccaacga tgctccggcg
     3961 ctggcgcagg cagatgtcgc ggtggcgatg aactccggta ctcaggcggc gaaagaagcg
     4021 ggcaatatgg tcgatctcga ctctaacccg accaagttga tcgaggtggt acatattggc
     4081 aaacagatgc tgatgacccg tggctcgctg accaccttca gcattgccaa cgatgtggcg
     4141 aaatacttcg ccattattcc ggcggcgttc gcggcaacgt atccgcagtt aaatgcgctg
     4201 aacatcatgc gccttcattc gcccgactcc gccattctca gtgcggtcat tttcaacgcc
     4261 ttgattatcg tctttttgat tcctctggcg ttaaaaggcg tgagttataa accgcttacc
     4321 gcttctgcca tgttgcgccg taacttatgg atttacggtc tgggtgggct gctggtgccg
     4381 tttattggta tcaaagtcat tgatttactg ctgaccgttt gcggtctggt gtgaggttta
     4441 ccatgcgtgg attacgcccg gcattatcga catttatctt tctgttattg attactggcg
     4501 gcgtttaccc gctgctgacc accgtgctgg ggcaatggtg gtttccctgg caggccaacg
     4561 gttcgttgat ccgtgaaggt gatacagtgc ggggttcggc attaatcggg cagaatttta
     4621 ccggcaacgg ctattttcat ggtcgcccgt cggcaacggc agaaatgccc tataatccac
     4681 aggcttctgg cgggagcaat ctggcggtca gtaaccctga gctggataaa ctaatagccg
     4741 cacgcgttgc tgcattacgg gccgctaacc cggatgccag cgcgagcatt ccggttgaac
     4801 tggtgacggc atcggcaagc gggctggaca ataatatcac cccgcaagcg gcggcctggc
     4861 agatcccacg catcgcgaaa gcgcgtaatc tcagcgttga acagctcacg caactgatcg
     4921 caaaatacag ccaacaacca ctggtgaaat atatcggcca gccggtcgtc aacattgtca
     4981 aactcaatct ggcgctggat aaacttgatg aataacgaac ccttacgtcc cgaccccgat
     5041 cgtctgctgg aacaaactgc cgcgccgcat cgggggaagc tgaaagtttt cttcggtgcc
     5101 tgcgcaggcg tcgggaagac ctgggcgatg ctggcagaag cccagcgact gcgggcgcaa
     5161 gggctggata ttgtggttgg cgtggtagaa acccacgggc ggaaagatac cgccgccatg
     5221 ctggaagggc tggctgttct gccgccaaaa cgcctggcgt accgtgggcg gcatatcagc
     5281 gagtttgatc tcgatgccgc cctcgcccgc cgtccggcgc tgatcttaat ggacgaactg
     5341 gcgcacagta atgcgcctgg ttctcgtcat cccaaacgct ggcaggatat tgaagagctg
     5401 ctggaagcag gcattgatgt ttttactacc gtcaacgttc aacatctgga aagcctgaat
     5461 gatgtggtca gcggcgtcac cggaattcag gtacgggaaa ccgtgcccga tccttttttc
     5521 gatgccgccg acgacgtagt gctggtggac ttgcccccgg acgatctgcg ccagcggctg
     5581 aaagaaggca aagtctatat tgccgggcag gcggagcgcg ccattgaaca ttttttccgc
     5641 aaaggcaatc tgatcgccct gcgtgaactg gcactgcgcc gtactgccga tcgcgtagat
     5701 gagcaaatgc gcgcctggcg ggggcatcct ggtgaagaga aagtgtggca cacgcgcgac
     5761 gcgatccttt tatgcatcgg gcataacacc ggcagtgaga aactggtccg cgcggcggcg
     5821 cggttggcct cgcgcctggg tagcgtctgg cacgcggtgt atgttgaaac ccctgccctg
     5881 catcgcttac cggaaaaaaa acgtcgggca attctcagcg ccttacgtct ggcgcaggag
     5941 ctgggcgcgg agacggctac actttctgat ccagcggaag aaaaagcagt agtgcgttat
     6001 gcccgtgaac ataatctcgg caagattatt ctcggtcgcc cggcctcgcg ccgctggtgg
     6061 cgtcgggaaa cgtttgctga ccgactggcg cgcatcgccc ccgatctcga tcaggtgctg
     6121 gtcgcgcttg atgaaccacc cgcccgcacg attaacaacg cgccggatag ccgctctttt
     6181 aaagacaagt ggcgggtaca gattcaggga tgcgtggttg ccgccgcgtt atgcgccgtt
     6241 atcactttaa ttgccatgca gtggctgatg gcgtttgatg ccgccaacct ggtgatgctg
     6301 tatctgcttg gcgtggtggt ggtggcgcta ttttatggac gctggccttc ggtggttgct
     6361 accgtcatta atgtggtgag tttcgatctc ttttttatcg ccccacgcgg cacgctcgct
     6421 gtctctgatg tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg gctggtgatc
     6481 gggaacctta ctgcgggtgt gcgttatcag gcgcgggtag cccgttaccg cgagcaacgc
     6541 acacggcact tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc gcaggacatc
     6601 gccgccacca gcgaacaatt tattgcctcc acgtttcatg cccgcagtca ggtgttgttg
     6661 cccgatgaca acggtaaatt gcagccgtta acacatccgc aaggaatgac gccatgggac
     6721 gatgccatcg cgcagtggag ttttgataaa ggcctgcctg cgggtgcggg caccgacacg
     6781 ttacccggcg tgccgtacca gattttgccg ctaaaaagcg gcgagaaaac ctacggactg
     6841 gtggtggtgg aaccggggaa tctgcgtcag ttgatgatcc cggagcagca gcgcctgcta
     6901 gagacgttta cgctgttagt cgccaatgcg tttgagcggc tgacgctgac cgccagtgaa
     6961 gaacaggcgc ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcggcgctt
     7021 tcgcatgatt tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat cttaacgctc
     7081 gatctggcaa gcgaaggatc gccccacgcc cgccaggcca gcgagattcg tcagcatgtg
     7141 ctgaacacta cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc
     7201 tttaatttga agaaagagtg gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg
     7261 ctggaaccgg gtttatcgtc gcccatcaat ctttctctgc cagaaccgct gaccttaatc
     7321 cacgttgacg ggccactctt tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa
     7381 tatgcgggtg cgcaggccga aattggtatc gatgcccacg ttgagggcga aaatctacaa
     7441 ctggatgtct gggataacgg ccccggtctt ccgccaggcc aggagcagac gatatttgat
     7501 aagtttgctc gcgggaataa agagtcggca gtaccggggg tagggcttgg actggcaatt
     7561 tgtcgggcga tagtggatgt acacgggggc actattaccg cgttcaaccg accggaaggt
     7621 ggtgcctgtt ttcgtgttac acttccccag caaaccgctc ctgaacttga agactttcat
     7681 gaggatatgt gacaaacgtt ctgattgttg aagatgaaca ggctattcgt cgctttctgc
     7741 gcacggcgct ggagggcgac gggatgcgcg tctatgaggc cgaaacgctg caacgcggct
     7801 tgctggaagc ggctacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg
     7861 gtgatgggat tgagtttatc cgcgacctgc gccagtggag cgcggtgccg gtgattgtgc
     7921 tttccgcacg cagcgaagag agcgacaaga tcgccgcgct ggatgccgga gcggatgatt
     7981 atctgagtaa gccgtttggc attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc
     8041 gccactccgc caccgccgcg cccgatccgc tggtgaaatt ttccgatgtt accgtcgatt
     8101 tagccgcccg cgtgattcac cggggtgagg aagaggtgca tctcacgccg attgagttcc
     8161 gcctgctggc ggtgctgctg aataatgccg ggaaagtgct cacccagcgc cagttactca
     8221 atcaggtgtg ggggccaaac gcggtcgaac acagtcacta tttgcgtatt tatatgggac
     8281 atctgcgaca aaaactggaa caggatcccg ctcgcccccg ccatttcatt actgaaaccg
     8341 gtattgggta tcg
//
LOCUS       DAZFRN010000189         8317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFRN010000189 DAZFRN010000000
VERSION     DAZFRN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.191"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            16..339
                     /gene="yiaW"
                     /locus_tag="ACHKUB_004404"
     CDS             16..339
                     /gene="yiaW"
                     /locus_tag="ACHKUB_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6657335.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            345..1481
                     /gene="yiaV"
                     /locus_tag="ACHKUB_004405"
     CDS             345..1481
                     /gene="yiaV"
                     /locus_tag="ACHKUB_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6657336.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1478..2452)
                     /gene="yiaU"
                     /locus_tag="ACHKUB_004406"
     CDS             complement(1478..2452)
                     /gene="yiaU"
                     /locus_tag="ACHKUB_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6657337.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            2576..3316
                     /gene="yiaT"
                     /locus_tag="ACHKUB_004407"
     CDS             2576..3316
                     /gene="yiaT"
                     /locus_tag="ACHKUB_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO6657338.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSIFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(3263..3526)
                     /locus_tag="ACHKUB_004408"
                     /pseudo
     CDS             complement(3263..3526)
                     /locus_tag="ACHKUB_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3530..3631
                     /locus_tag="ACHKUB_004409"
                     /pseudo
     CDS             3530..3631
                     /locus_tag="ACHKUB_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(3663..4358)
                     /gene="araD"
                     /locus_tag="ACHKUB_004410"
     CDS             complement(3663..4358)
                     /gene="araD"
                     /locus_tag="ACHKUB_004410"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6657339.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHTT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(4352..5212)
                     /gene="sgbU"
                     /locus_tag="ACHKUB_004411"
     CDS             complement(4352..5212)
                     /gene="sgbU"
                     /locus_tag="ACHKUB_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO6657340.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(5205..5867)
                     /gene="ulaD"
                     /locus_tag="ACHKUB_004412"
     CDS             complement(5205..5867)
                     /gene="ulaD"
                     /locus_tag="ACHKUB_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6657341.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(5864..7361)
                     /gene="lyxK"
                     /locus_tag="ACHKUB_004413"
                     /pseudo
     CDS             complement(5864..7361)
                     /gene="lyxK"
                     /locus_tag="ACHKUB_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
     gene            complement(7365..>8317)
                     /gene="yiaO"
                     /locus_tag="ACHKUB_004414"
     CDS             complement(7365..>8317)
                     /gene="yiaO"
                     /locus_tag="ACHKUB_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO6657342.1"
                     /translation="AGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNDLLQERTK
                     GELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTA
                     HAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLKGLKIRTNN
                     SPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQKYLSLTH
                     HAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQKIIDGMKEAGVE
                     VITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
BASE COUNT         1857 a         2186 c         2224 g         2050 t
ORIGIN      
        1 ttctttgagg gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt
       61 ctggtgatct tctatgggat cattatttta catgatattc cctacctgat tgccaaaaaa
      121 cgtaatcatc ctcatgccga cgccattcat gttgctggtt gggtgagtct ttttacgtta
      181 catgttatct ggccgttttt gtggatttgg gccacgcttt accgcccgga gcggggatgg
      241 ggaatgcaaa gccatgattc atccgttatg caactgcaac agcgcattgc cgggctggag
      301 aaacagctcg ccgacatcaa atcctcttct gccgagtaat acttatggat ttactgatta
      361 ttttgaccta tgtggctttt gcatgggcaa tgtttaagat cttcaaaatt cccgtaaata
      421 aatggaccat tcccacagcg gccctgggtg gaatatttat tgtcagcggt ctaattctgt
      481 taatgaacta taaccatccg tataccttca aagcgcaaaa agcggttatt tctattcctg
      541 ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat acgctgatta
      601 aaaaaggtga ggtgctattt cgactggacc cgacgcgtta tcaggcgcgg gtggatcggc
      661 tgatggcgga tatcgttacc gcagaacata aacagcgggc gttgggcgca gagttagatg
      721 agatggcggc gaatactcag caggcaaagg ccacgcggga taaattcgct aaagagtatc
      781 agcgttacgc acgcggcagc caggcgaaag taaacccgtt ttcagaacgc gatattgatg
      841 tggcgcggca aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa
      901 aacagatcca gagccagctg gatagcctgg tgttgggtga acattctcaa atcgccagcc
      961 tgaaagcgca gctcgcggaa gcaaaatata accttgagca gacgatagtg cgtgcgccga
     1021 gcgatggtta tgtgacccag gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc
     1081 tacgtccggt gatggtgttt atacccgatc agaaacgaca aattgtggcg cagttccgtc
     1141 agaactcctt gctgcgactg gcccctggcg acgatgcgga agtggtgttt aacgctctgc
     1201 caggcaaagt gttcagcggt aagctggcag ccattagtcc agctgttccc ggcggagctt
     1261 atcagtcgac cggcacctta cagacgttaa acacagcgcc gggttcagat ggcgttatcg
     1321 cgaccattga actggatgag cacactgatt tgagcgcttt accagacggt atttacgccc
     1381 aggtggcggt ctactctgat catttcagcc atgtctcggt gatgcgcaaa gtgctgttac
     1441 gcatgaccag ttgggtgcat tacctttatc tcgatcatta atcttcgctt tccgccgctt
     1501 gttcaggccg cagttgctgc gactgagcaa gctggtggcg aatttcacgc agcatttcat
     1561 ccagcacata ctggacccgc cagggctggt attcccgttt acggaagata gccaccagat
     1621 ccagccacca ttcatacggg tgctgaaagc agggcaccag cgtgccgtct tgcagatcgc
     1681 tctgcacact tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc agctccagcg
     1741 ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc acctctttac
     1801 tcccggcgac gttgaagcgc cagatattcg cgtcatcaat catcatcgag tcgatcaaaa
     1861 tacaggagtg atgttcgagt tcgtcaggac ggctaatagg atgtttctca agatagcgct
     1921 ggctggcata agcggttacg gcatattggg taataaaact ggcaaccagc gattcatctt
     1981 ttggtggagc gtagctgatt aaaacatcac aatcatcagg aaattcgacg ccttcataaa
     2041 aggcattacg ctcaagattg caggttttta gcgataaggt gatatcaccg atatctttaa
     2101 ttttatcgat tacatgtttg gataaatagg taattatgcc ggttggggcg tagatggtga
     2161 cccgaccacg tttctcatgc ttataatctg caataaaatt attaagctgc tcgtttctgt
     2221 ccagcatgtc gttgatgtac ggtaacagcg cggtaccaaa aggtgtgagc atcagctgcc
     2281 gggtggttcg gtcaaagact tttaaaccca cttttgattc aaaatcagca agatatttgc
     2341 tgacgttggc ctgtgcgatg ccaagtacag tcgcggcatg gctgatattt tcactggcag
     2401 cgattaccga gataattttt aactcccggt acttaagttg taattttgtc atcgcaacag
     2461 cccaattata tataatttta tatattacta tataaatatg aatcttgcac cggagaaatt
     2521 gtaagcatta ctatgccgac tcttttttat cttattttaa aatggaatac cgaacatgtt
     2581 aattaaccgc aatattgtgg cgttatttgc gttgcctttt atggcaagcg caactgcttc
     2641 tgaattatcc attggtgccg gtgcggctta taatgaatcg ccttatcgcg gttataatga
     2701 aaatacaaag gcaattcccc tgattagtta tgaaggtgat tctttttatg ttcgtcagac
     2761 cacgttaggt tttattctgt cgcaaagtga aaaaaatgaa cttagcctga ccgcatcctg
     2821 gatgccgctg gaatttgacc ctgccgataa tgacgattat gccatgcaac agcttgataa
     2881 gcgtgatagt acggctatgg cgggggttgc ctggtatcac cacgagcgtt ggggaactgt
     2941 gaaagcctct gcggctgcgg acgttctgga taacagcaac ggctgggtgg gggagctatc
     3001 gattttccac aaaatgcaga taggtcgcct gtcgctgaca cctgcgctgg gcgtgctcta
     3061 ttatgacgag aatttcagtg actattacta tggcatttca gagagtgagt cccgtcgtag
     3121 cggtctggca agttattccg cgcaggatgc ctgggtgccc tatgtcagcc tgacggcaaa
     3181 atacccgata ggagagcacg tcgtattgat ggcgagtgca ggatacagcg agctgccgga
     3241 agagattacc gacagcccga tgattgatcg taatgagagt tttacctttg tcaccggcgt
     3301 gagctggcgt ttttaattca ccggtggatg tcggtgcggc tggtgctccg tcgtaatgcc
     3361 acaaaggttg ttgctctgga ttgctctgtt cataccggga acctggcgtc tgaatcagga
     3421 tcttatggct gaacaatccc ttgccttcaa atgtcgtaaa tggcgcatcg gcgggcagca
     3481 acaggttatg cagtgactcg acgttgtcgg cctgctccat gcaatacggt ttaatgacgt
     3541 tggctatttc cggcagattt ttcgcaagca caccggttta acgccagcag cgtggaagcg
     3601 gcgttacagc aaggaacata tcaattcgta gtgccggggc gatgaagccc cggcgtgagg
     3661 gattactgcc cgtaataggc attggcccca tgcttacgca ggtagtgctt atccagcagt
     3721 tcgttttgca tcgcagggag ctgcggcgca agctggcgcg agaataggcc catataggcg
     3781 cattcttcga gtactacggc gttatgcacg gcatcggcgg cgtttttacc ccatgcgaac
     3841 gggccatgag aatgcaccag caccgccggg atttgtgccg gactcctgcc acgttcttcg
     3901 aaggtttcaa tgatcacttc gccggtctga tattcatatt cgccgttaat ctcctctgcg
     3961 gtcatctgtc gcgtgcaggg gatggcacca tagaaataat cagcgtgcgt ggtgccccag
     4021 gcggggagat ccagcccggc ctgcgaccag atggtggtgt ggcgtgagtg ggtatgcaca
     4081 atgccaccga tttcggcgta gcgacggtaa agcgccagat gcgttggcgt gtcggacgag
     4141 ggctttttgc taccttccac caccttgccg ctggctatct caaccaccac catatcgtcg
     4201 gcggtcatca cgtcgtactc gacgccggaa ggtttgatta ccatccattg ccgcgtgtcg
     4261 tctaccgcgc tgacattgcc ccaggtgaac gtcaccagat ggtgagcggg aagcgccaga
     4321 ttcgccgcca gcacgtcggc tttcagttgc tctaacatat aaatccagcc tcctgcatac
     4381 gcgcttcaat ccagcgccgc gcctgaatga tctccagcac cggctctttg gctttttcgg
     4441 tccacatctc aatcaggaaa gaaccgcgat agttcagttc atgcagcgtt ttgaagatgc
     4501 caacgaaatc gacgcagcct tcgccaaacg gcacatcacg gaactgtccg gggctttgcc
     4561 cggtgactgg ctgggtgtct ttcaggtgga tcgcggcgat acggtcaatg cccagtttca
     4621 gttcggcggg aacatcattg ccccaggcgc tgaggttgcc gacgtccggg tagacggtga
     4681 accacggcga ggcgagcatc tcgtcccatt ttttccattt gctgatggag ttcataaacg
     4741 ctgtatccat aatctccacc gccagcatta cttgtgatgc cgccgcctgt tcgactgccc
     4801 acgccagccc ttcagcaaaa cgttgccggg tgccttcgtc gtggtcttcg taatagacgt
     4861 cataacctgc cagctggatg gtgcgaatgc cgagatcgcg cgccaggcga atcgctttac
     4921 tcatgatttc ccgcgcccgt tcgcgcactg cctcgtcacg gctaccaaag ggaaagcgac
     4981 gatgtgcgga caggcacatc gacgggatcc caacacctgt ttcgatcatc gcggcaacca
     5041 gcgaagtcct ttgtgcggcg ctccagtcaa gacgtgagag ccgttcgtcg gtttcatcca
     5101 ccgacatttc gacaaaatca aaaccgcagc tttttgccag caccagccgc tccggccagg
     5161 agagatcttt cgccagcgct ttttcataaa tccctaacgg atgattacgc acgcgcacct
     5221 ccccagatag cgtcgatttg cgcatggaaa tcgccagcca cttgcgccgg atttgccgcg
     5281 cctgccagtg cccgcccggc aataaacgct ttcacgcgga tatctttaaa cagcggcagg
     5341 tcagcagggg taatcccgcc agtaatggaa agctcaaggc cgatatctga aagcgccttc
     5401 atgcgtgcca gatcggcttc gccccactgt tgcccgctgg cctgtgcatc acgaccgcga
     5461 tgataaatgg cctgccgcac gccaatacga tgccagtcgc gggcgtcgtc cagcgtccag
     5521 ttaccgaaca gctctatctg aatttcaccc ccgcagcgtt gtgccattgc gtggcctttt
     5581 tctaccgtcg cgagcggcgc ggcgcagatg atggtcatcc agttagcgcc tgcgccaaac
     5641 gcctgttgcg cgagcgtttc accagcgtcg gcgaccttcc agtcagcaac gatgattttg
     5701 tccgggcact gttcgcgcaa ggctttcacc gcgccaagcc cttcgtttaa acagagaatg
     5761 gtgcccgctt cgacaatatc gacgctgtct tttaactgcg tcacgtcgcg ctgtgcggct
     5821 tcaagtgatg agtggtcgag tgccagttgc agaagtggtc ggctcataat atgtgctcct
     5881 tgatgcgggc gtgaaagccc tgaagtgcgg caatgagatg ctgatagcgt tggtattttt
     5941 gttggtaaag ctgatgggcg gtcatatccg gcagcagggt gcgtaccggg tgctgtaagt
     6001 cacgttgagc ttcgctgaaa tcgcgataaa ctccggtgcc gacacgggcg gcaagtgccg
     6061 cgccaaaaca gccggtttct tccacctgcg gcagctcgat acgcagaccg ctgacgtccg
     6121 ccagcatttg catccagaca tcggagtgcg ccgggccgcc agtgacgcgc agggtgtgca
     6181 catcagtaaa acgttcgcgc atccggttga ggtgggtcat atggctgaac accaccccct
     6241 tcataaatgg cctgcaacag gtgtgcgcgg gtgtgaatgg cctgcatccc gtagaaaccg
     6301 ctggtcatct cgagtccggc gttgctgccg tacaggaacg gcaggaaaaa gagatcgcca
     6361 ccggccttcg gcaagctggc aacggcctgg ttgatctcat caaacgagat ttctccccac
     6421 tgtgcggtaa accattcgag gttgccggaa gaggtggggc tggcttcgtg aacgataaat
     6481 tcaccatcgt taacgtagcg accatagaca tacggatgcg cttcaccgtc acgtaaaccg
     6541 cgggttatgc cgctggtcac cgcccaggtg cccatcaccg cattaagggt aaattcgtct
     6601 tcgatcccgg cgcagagtgc ggtggaaacc acatcaaaca ggccgccgac aacgggcgta
     6661 cccgctttca gaccggtcag tacggctgtc tgagcggtga tctccccgca gatttcggca
     6721 gatccgacaa caggcggcag ggcgtgattg atttcagcaa tccccagcca gtcggtgagg
     6781 cacgggtcat attccccaag actcatgttg tagaggttgg actcggaaat attgctctct
     6841 tcacagcctt tgacgccggt taaacaccag cgcaggtagt cgtgcgtcat catcacgcag
     6901 ccaatttgcg cgtagcgttc cggttcgtgc tctttcagcc agcgtaacag cgacaccgga
     6961 tgcccggtcc acaaggtttg tcgggtcaac gggtagagtt tttccgggat gccatcttcc
     7021 tgccagcgac gaacgatttc catcgcccgg cgatccgagg acaaaatggc gttaccgagc
     7081 ggtttgttgt ttttatccag caaaaacaag ccttttcctt gtgcggagat accgataccg
     7141 acaatttgtt ccccgctaac gccggaatgg gtaagcagtt cgcgaatgac agccatgcag
     7201 cattgccaca gttctgccat atcgcgctct gcccaacctg gctgcgggct taatgcgcac
     7261 agcggcaggc gctgcacgcc tgcctcccgg ccttcgcggt catacagccc ggctttcagc
     7321 cagctaccgc cacaatctaa ccccagccag tattgcgtca tggtttattg cacctcatcc
     7381 acggctttca gtagatcagc accctgcgga acatctttta caaacatgtc gcggacctga
     7441 gtacccagtg cgtcgctaaa ggctttgcgg tcgagatcgg tgatgacttc cacgcccgct
     7501 tctttcatgc cgtcgatgat tttttgctga tcttcggcaa ccagtttgcg ctggtagtta
     7561 cccgcttcct gcgcagatga aattagcgct tgctggaact ccgggcttaa gccatcaaac
     7621 ttcgctttgt tgatcaccac cagaagcggg gaataggcgt ggtgcgtcag agaaaggtac
     7681 ttctgcactt cgaaaaattt tgctgaccag acgacgttga tcgggtgttc ctgagcgtcg
     7741 atagtgcggg tttccagccc ggtatagact tcggcaaacg gcatcgggat cgggttagcg
     7801 ccaaagactt tgaatgcggc gatattcatc gggctattgt tggtgcgaat tttcagccct
     7861 ttcaggtcgg cgggggtttt aaccggtgcg cgcgagttgg tgacatcgcg ccagccgttt
     7921 tcccagtagg ccagtacttt tagtccttta ccttcaagtg aggctttcag atcatcaccg
     7981 actttgccgt cgagcgtttt atgcgcgtga gcggtatcgc ggaacaggaa agggacatca
     8041 agcaagttca tcactggtga taacccggca aagttattcg agccggacat ttccatatcg
     8101 atggtgccgc cacgtacgcc gctgatcatc gcctgcgcgt taccgagggt gctatccgga
     8161 aacaatttta atttcagctc gcctttggtt ctctcctgca ataaatcatt gaattttttc
     8221 gccgcaatat gttgcgagtc ggtttgtgag gtttcataac cgaaacgtaa agattgcgcc
     8281 gccagagaag atgtgctgaa tgcagccagg ccagcaa
//
LOCUS       DAZFRN010000190         8315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFRN010000190 DAZFRN010000000
VERSION     DAZFRN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.192"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..286
                     /locus_tag="ACHKUB_004415"
     CDS             <1..286
                     /locus_tag="ACHKUB_004415"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6657343.1"
                     /translation="IMTLKEFIKSLKTGEAKKFATNLGVSPSFLSQMASGTSPVSPVR
                     ALMIEAETGGHVTRRELRPNDWELIWPEYTGITSPSRHKYELNTKKADGG"
     gene            290..715
                     /locus_tag="ACHKUB_004416"
     CDS             290..715
                     /locus_tag="ACHKUB_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6657344.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEIARIYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTSDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAVAIAWGTLTNSGGQPGNAVVVH"
     gene            784..1815
                     /locus_tag="ACHKUB_004417"
     CDS             784..1815
                     /locus_tag="ACHKUB_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO6657345.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDTE
                     LNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVW
                     GMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVWAQVKPVSKGVSHA
                     ASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEA
                     LDCPDWCDD"
     gene            1808..2269
                     /locus_tag="ACHKUB_004418"
     CDS             1808..2269
                     /locus_tag="ACHKUB_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6657346.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            2303..3019
                     /locus_tag="ACHKUB_004419"
     CDS             2303..3019
                     /locus_tag="ACHKUB_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6657347.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVQETT
                     TQEEMKTEIVEDIVKLQPSVTETKADDLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            3052..3333
                     /locus_tag="ACHKUB_004420"
     CDS             3052..3333
                     /locus_tag="ACHKUB_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6657348.1"
                     /translation="MILRQCAGTMKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     QSAKYSQRDIEQARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVQA"
     gene            3330..3635
                     /locus_tag="ACHKUB_004421"
     CDS             3330..3635
                     /locus_tag="ACHKUB_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6657349.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKREGHTVLNPSVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEWD"
     gene            3622..3966
                     /locus_tag="ACHKUB_004422"
     CDS             3622..3966
                     /locus_tag="ACHKUB_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6657350.1"
                     /translation="MSGINYQALREAAQNYQSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            3968..4321
                     /locus_tag="ACHKUB_004423"
     CDS             3968..4321
                     /locus_tag="ACHKUB_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657351.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAKP
                     VASCIIEDGGMCVDGFGEYVGHSLPDGTHQLYAAPPVPSKLPREYRNGWPLAYSDYAE
                     GWNDCREAMLQGDKS"
     gene            4318..4671
                     /locus_tag="ACHKUB_004424"
     CDS             4318..4671
                     /locus_tag="ACHKUB_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6657352.1"
                     /translation="MINRIKLEHILEYARQQKHTGQHCKIPPRDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPPVGVDVLLFVSSTGEQFTGFNLDNTGDFQYAQCFGAPIVCEA
                     THWMPLPEPPKEVRQ"
     gene            complement(4874..5173)
                     /locus_tag="ACHKUB_004425"
     CDS             complement(4874..5173)
                     /locus_tag="ACHKUB_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6657353.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNIIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(5179..5436)
                     /locus_tag="ACHKUB_004426"
     CDS             complement(5179..5436)
                     /locus_tag="ACHKUB_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO6657354.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            5572..5850
                     /locus_tag="ACHKUB_004427"
     CDS             5572..5850
                     /locus_tag="ACHKUB_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657355.1"
                     /translation="MAQDTKLHNSDNSAAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            5852..6901
                     /locus_tag="ACHKUB_004428"
     CDS             5852..6901
                     /locus_tag="ACHKUB_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6657356.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAVWMIDVIRHAINGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPVEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVASIAVDPESPESFMKRPKHRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            6915..7289
                     /locus_tag="ACHKUB_004429"
     CDS             6915..7289
                     /locus_tag="ACHKUB_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6657357.1"
                     /translation="MCIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVAFIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            7286..8107
                     /locus_tag="ACHKUB_004430"
     CDS             7286..8107
                     /locus_tag="ACHKUB_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6657358.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGRMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT         2175 a         1872 c         2385 g         1883 t
ORIGIN      
        1 aattatgacg ctaaaggaat tcatcaaatc attaaaaact ggagaagcaa aaaaatttgc
       61 gaccaatctt ggtgtgtctc cgtctttctt gtcccaaatg gcatctggaa cttcacctgt
      121 gtctcctgtt cgtgcgttga tgatagaagc agaaactgga ggacatgtta caagacgaga
      181 attgagacct aatgactggg agcttatatg gcccgaatac actggaatca cgtccccttc
      241 acgtcacaaa tatgaactta acaccaaaaa ggctgatgga ggttgattca tgaaaatcaa
      301 gcatgaacac atccgcatgg cgatgaatgc ctgggcacat cctgatggtg agaaagttcc
      361 cgcagctgaa atagcccgga tttatttcga gctggggatg acgttcccgg aactgtacga
      421 cgacagccat ccggaagccc tggctcgtaa tacccagaaa attttccgct gggtggagaa
      481 ggacacgtct gatgctgttg aaaaaattca ggcgctgtta ccggcgatcg aaaaggcgat
      541 gccgcctttg ctggtggccc gtatgcgcag tcacagttct gaatattacc gggagatcgt
      601 cgaacgacgg gatcggctgg tgaaagatgt ggatgatttt gtcgctgtag cgatcgcctg
      661 gggcaccctg actaacagtg gtggtcagcc tggtaatgca gttgttgtgc attgaccaac
      721 aatatttata ccggatttct tccggaagtt cgtgggtaaa gttcggtatc agaagaggtg
      781 agtatggcta atgcctggct cagattgtgg catgacatgc caaatgaccc caagtggcga
      841 acgattgcca gggtatcagg acagccaatc gcaacagtga tggcagtgta tatccacctt
      901 ctggtgagcg cgtcacgaaa tgtcacgaca tgtcacggcg tgtcactacg tggtcacatt
      961 gatgtcacga cggaagattt agcaagtgcg cttgatgtga cggaagacgt aattgattca
     1021 attttgcatg caatgcaggg gcgggttctg gatggtgacc ttatttccgg atgggaaaaa
     1081 cgtcaggtgc tgaaagagga caatggtaac gtttcgcaaa cggcaaaatc cccggcagag
     1141 cgtaagagag cgcagcggga gcgggaaaag ctgcggaaac ataatgctga ttgtcacgat
     1201 gagtcacgac gtgtcacgca tctgtcacga caagtcacga cagataaaga tacagataca
     1261 gaattaaacc ccacacataa cgcgcgcgag agtattccga ccagtgagtc gaatggtgcg
     1321 ccgttgcaga cagccgaacc tgaatacctg gacggcctga gcgaacccat cgggaaattt
     1381 tcgatgacta ctgtctggca gccgtcgccg gattttcgac aacgggcagc agtgtggggt
     1441 atggctctgc ctgagccgga atttacacct gctgagcttg ccgcattccg ggattactgg
     1501 atggcagagg ggaaggtttt cacgcaggtt cagtgggagc agaaatttgc ccgtcacgtg
     1561 cagcacgtct gggcacaggt aaaaccagtc agcaaggggg taagccatgc agcatcaggt
     1621 ggcacggcat cacgggcagt tcaggaaatc cgggcagcac gcgaacagtg ggaacgtgac
     1681 aacggattta tcagcaacgg aaacggcctg gaagctgtgg gagcttatgg gggaggtgta
     1741 ttcgaaccgc tggactcaga agaacggggc cgcaccttcg aagctctgga ttgcccagat
     1801 tggtgcgatg actgaacagc aaatccgtct ggtctgccgt cagtgcatgg accgctgccg
     1861 ggcgggtgaa acgtggcccc cggacctggc tgagtttgtt gcactgattt cggagagtgg
     1921 ggcaaatcca tttggtctta cggtggatgc cgtgatggaa gagtaccggc gctggcgcaa
     1981 tgaatcctgg cgatacgacg ggagtgataa atacccgtgg ccacagcctg tgctgtacca
     2041 catctgcctc gaaatgcgta ccagagggat tgagcgccag atgacgcagg gtgagttaaa
     2101 acgacttgcg gaacggcaac tgacgaaatg ggcaaagcat gttggtaacg ggatgagtgt
     2161 tccgccagtg cgacgacaac tggaaggggc gaaacacccg caagggccaa cgccaattga
     2221 acggctgaaa caggaatacg aacgccggaa ggctgctggt tttatttgaa tctgggaaac
     2281 gattttgtcg gaggaaattt taatggaaac cgtatttgac gcactgaaag cactgaaaag
     2341 agcctcttca caggtagtgg cggcccgcct tggaatcagc cgtgaagatg cggtcaacga
     2401 actgtggaaa ctgaagcgcc gtggtgaagc ggataacaag gggtcgatgt ggtggctgac
     2461 tcaggcaggt gaaagtgaac cagtgtcacc ggtaccgaaa gtgacagcgc aaatgctgac
     2521 tgaggcgatt gaacaacatg gcccacaaac ggcggatgag ctggcactta tgttcgggat
     2581 tacctcccgc cgggcgaatt catcgctggc catggcaatc agcaaagggc gtctgattcg
     2641 cgtaaatcag ggcggtaaat ttcgttactg cataccgggc gctgatttac cggcagagcc
     2701 ggaagctgca tccgtagcgg aaaccgatgg taaagccttt cctcagccag caggtgttgc
     2761 gttaccagtc caggaaacga cgacacagga agaaatgaaa acagaaatcg tggaagacat
     2821 tgtgaagtta cagccatcgg tcaccgaaac gaaagcagat gacctgattc taccatcgct
     2881 gcatgtggct aaccgcgagc tgcgccgggc aaaaggtcag gttcagaagt gggagcgagt
     2941 ctgcgccgcg ctgcgggagc tgaacaagtg ccgggatatt ctccgggata ttaccgccac
     3001 cagagaacag cagcggtgag tgggtggaag acgtggtgcc gggctgaaat tctgatactc
     3061 cggcagtgcg cgggaacaat gaaggtaaaa agcattggcg cacttatcgg acgaactgaa
     3121 gcggcagtga gaacgaaggc acgggagttg ggcatcagca tgatgttacg tggtgatttt
     3181 caccagtcag caaaatattc tcagcgtgat attgagcagg cgcggcaact gcatcagaga
     3241 ggcatgcaaa gaagggaaat tgccagaaaa ttaggcatgc cgctgcgcat agtgaataac
     3301 tacgtttatt tcgacaggag ggttcaggcg tgagggtgag aatttatatc gccggtccaa
     3361 tgacgggata tgaaaatttc aaccgcgagg catttcacaa ggcggaagaa gcactgaaac
     3421 gggaagggca taccgtttta aacccgtcag tacttccgga cgggctgact cagccacatt
     3481 acatggatat ttgcatggca atgattcgtt gtgtggatgc ggtttatatg ctgaaaggct
     3541 ggcagcggtc ggcaggcgct aaggcagaac tggcactggc ggagaaactg gggcatgcag
     3601 ttattttcca ggaggcaacc agtgagtggg attaattatc aggcgctgcg tgaggcggca
     3661 caaaactatc agtcgatgct ggcgtggtat caggagaaac cagacagtcc aaacgctgag
     3721 caggattgtg atgcagcttt ggctgcgttt aagcgtgaaa tccgtcatcg tgaagtggat
     3781 attatcgctg atttgctgga tgaactggag gaagcaaaac agcgtatcga cgagcaggag
     3841 tcccgcatag tgaagttacc agaaccattc aagctggcta aatcatcaag tggattaacg
     3901 tactactacg ctgatgaggt caatgccgca ctgactgctg ctggtattcg catagaagga
     3961 gagtgagatg accactatta ccaaagagcg actgcaatgg ctggctaaca tttctggccg
     4021 cgatgacatt gacgatatag acggcggtga aattcgtgag ctggcgctta tcgcgctggc
     4081 atcgctggaa gcgaagcctg tagcttcatg tattattgaa gatgggggca tgtgtgttga
     4141 cgggttcggt gagtatgttg gtcactcgct gcctgatgga acgcaccagc tttacgccgc
     4201 cccgccagtg cctagtaagt tgccgcgtga atacagaaac ggttggcctc ttgcatatag
     4261 tgattatgct gaaggctgga acgactgccg cgaagccatg cttcagggag ataaatcatg
     4321 attaatcgta tcaagctgga gcacatcctc gaatatgcca ggcagcagaa gcatactggt
     4381 cagcattgta aaattccacc aagggatatg gttgaaatca tggagattgc catgcgcaag
     4441 gctggcaatt ctccggtaac tccggatggt tggataagct gtagtgagcg aatgccgcca
     4501 gttggtgttg atgtactact gtttgtgtcc agcacgggtg aacagtttac aggttttaac
     4561 ctggataata cgggtgactt ccagtatgcg cagtgtttcg gtgcgcctat cgtgtgtgaa
     4621 gccacacact ggatgccgct accagaaccg ccgaaggagg tgcgccaatg agctggcctg
     4681 aggccttctc aaatgttggt attgcaatgg cggtggcgct ggtggtgtat tcgatttgcc
     4741 gctggggata aatcgccgaa aaaagatccc gccacaaaca tgagccggga tctttgattt
     4801 atatagccta cgaatccgca gtaagagagg ggcagacggt ttattctaac accggaatga
     4861 tgtgggtaaa agtttataag aaatcggttt cataactttg cccaccatga tagataccga
     4921 caataaagac ttttctgcca tcaacggcaa aagcaataat cgttctgtgg cggaaatgag
     4981 ttacccgcat cccctggcga atatcatcgc gtttattgcc ccgatgcggg aatgtagaaa
     5041 acccatcaag ataatcaaga agcgcattgg cataattgtc agcaatgatg ttccctgctt
     5101 tctccgttat atatctgtgc aggttgatta tttgttgttc ggcctcagga gtaatgatga
     5161 cttcatatgt catgcagatt acttcccgga tcgaatcgcg gcgcgaacct gtgaaatgga
     5221 gcgtccgttg tttgggtttt cgcggataga atcaagagag ggggcggctg aatgcgttaa
     5281 ccacgcttcg attgctttat cgcgctcatt cagtgcgcga agcccttcac gaatgacctc
     5341 gctttctgaa gcataggcac cggaagccac acgggcgcgc accatgtcag ccatctcgtt
     5401 agttaatgta atgctgaatt gttgggttgt acgcatggta aacctcacgg agtaggatag
     5461 aacactattc gatgatagca cgctgcctgt tgacgacaac agaaatcaga gacaatattg
     5521 ccgcacgcca gcctgaacaa ctggcacctg ctgcgccagc agagacaacc gatggcgcaa
     5581 gataccaaat tacacaattc ggataactcc gccgcccctg ccagcaggca cgggcggcgt
     5641 tctcatgcat tcaaatctga ctggttccag catgacccat gcactgaaga acaggccgaa
     5701 tggctgatcc agtgctaccg cagacgtggt tacgagtttc agaaagatct cagcttcgat
     5761 cgtcgtcact ggataatctc cgtcaggctc ccttattccg aacgcccacc gcgtccgtcc
     5821 cgcacattcc agcagcgtat ctggaggtaa cgtgcgggta ttacttcgac ctgttctggt
     5881 accggaactc gggatggtgc tccttaagcc gggccgtgaa tcaatgtcag cattccataa
     5941 cggcagaata ttggtggagc cggaaccgaa aaacatgcgc ggtctgccgt ccggagtcgt
     6001 tcctgccgtt cgccagccgc tggcagagga taaaacatta ctgccatttt tcagcgatga
     6061 gcgggttatt cgtgcagcag gtggtgcagg tgcactgtct gactggttat tacgtcacgt
     6121 gaaatcctgc cagtggccac acggcgatta tcatcacagc gaaaccgtca ttcaccgtta
     6181 cggtaccggc gcgatggtgt tgtgctggca ctgtgacaac cagctgcgcg accagacatc
     6241 agaatcactc gatcaacttg ctcagcagaa tctggcagtc tggatgattg atgttatacg
     6301 tcacgcaata aacggtacgc aggagaggga gttatcgctg gccgaattat cctggtgggc
     6361 ggcctgcaat caggtggtgg atgcactacc tgaggcagta gcgcgtcgtt cgctgggatt
     6421 accggtggaa aaaatccgct ccgtataccg tgagagtgac atcgtaccgg gagaacagac
     6481 agccatcagc atactgaagc agcgcacaaa aaatattgcg ctgccacttc acgtccacca
     6541 gcaacaaaat ccaccacaga aaaaaacggt tgccagtatc gccgttgatc cggagtctcc
     6601 tgaatcgttt atgaagcggc ctaaacatcg ccgttgggtt aatgagaaat acacgcgctg
     6661 ggtaaagaca cagccgtgtg cgtgttgtgg taagccagct gacgatccgc atcacctgat
     6721 tggtcatggt caggggggaa tgggaacaaa agcccacgat attttcacgc taccgctgtg
     6781 tcgggagcat cataacgagc ttcatgcgga tccgctggcg ttcgaagaaa agcatggttc
     6841 tcaggttgat ttaatttttc gttttcttga tcacgccttt gcaaccggtg tgcttgggta
     6901 aaaggggggt attgatgtgt atagagtttg ttttgcctta cccgccgacg gtgaatactt
     6961 actggcgacg tcgtggcagc acatattttg tatcaaaagc cggggagcgt tatcgccggg
     7021 cagtggcgtt tattgttcgc cagcagcgac tgaaattaag cctgtccgga cggctggcaa
     7081 taaaaattat tgcagagcca ccggataagc gtcgtcgtga cctggacaac attctgaaag
     7141 caccgctgga tgcgctgacg catgcgggac tgcttatcga cgatgaacag tttgatgaaa
     7201 tcaatattgt acgtggtcag ccagtatctg gtggacgtct gggggtgaag atttacccca
     7261 taatgcttga agggcaggtc aaaaaatgaa actggaagat ttaccgaaat actactcccc
     7321 aaaatccccc ggcctgactg atgcatcggc ctcaacgtca aaagatgcgc tgagtatcac
     7381 tgatgtgatg gccgcgcagg gcatgacaca gaatcgggct gagatggggt tttctgcgtt
     7441 ccttgggaga atgggcatta gtatgaatga cagagagcgg gcaacagaat tgctgacaga
     7501 atatgcactc agtcggtgtg atcgcgtggc ggcgttaaga aaactcccgg cagaaataaa
     7561 accggcagtg atgcgtatta tggcttcgta tgcgtttgaa gattatgccc gtagcgcggc
     7621 gagcaaaaaa cagtgcccct gctgtcacgg aaaaaaattt attgaaagcg aggtttttac
     7681 aaacaagatc cagtatccgg atggtaagcc gccagtgtgg gcaaagtgca caaaaggcgt
     7741 gtatccgtct tactgggagg aatggaaaaa agtcagggag gtggtaaaag ttgcctgtcc
     7801 ggagtgtggc ggaaaggggg aggtttccac cgcctgtaaa gattgtcgtg ggcgcggtgt
     7861 tgccattcat cgtgaagagt cggaaaaaca gggtgtgccg gttttcagaa actgccagcg
     7921 ttgtggtggg cgtggctatg aaagattacc ttcaacggag gcatttaatg ccatatgtaa
     7981 tgtaaccgat gccatatctc ttgatacatg gaaaaaaaca gttaaacgtt tttacgatac
     8041 gctggtggtg cagtttgata ttgaagaagc atgggcagaa caacaactga aaaaggtgac
     8101 cagatagctt tgttgatttt tcccgaatct gtggtaaatt tgccctaacg atgggcgttt
     8161 tatgcctgac gttagaagat ttttacaccc gtcgccaggc gggttttttt atgactgaaa
     8221 tcacgccagt acataaaatg tgcaggtggt tattaatacc ggtctttcag cttgctggct
     8281 tttttgacaa gagttattgg tgtgtcacgt taacc
//
LOCUS       DAZFRN010000191         8312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFRN010000191 DAZFRN010000000
VERSION     DAZFRN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.193"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            128..1906
                     /locus_tag="ACHKUB_004431"
     CDS             128..1906
                     /locus_tag="ACHKUB_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO6657359.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1946..2425)
                     /gene="xdhC"
                     /locus_tag="ACHKUB_004432"
     CDS             complement(1946..2425)
                     /gene="xdhC"
                     /locus_tag="ACHKUB_004432"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO6657360.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2422..3300)
                     /gene="xdhB"
                     /locus_tag="ACHKUB_004433"
     CDS             complement(2422..3300)
                     /gene="xdhB"
                     /locus_tag="ACHKUB_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO6657361.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3311..5608)
                     /gene="xdhA"
                     /locus_tag="ACHKUB_004434"
     CDS             complement(3311..5608)
                     /gene="xdhA"
                     /locus_tag="ACHKUB_004434"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO6657362.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVIINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6023..6778
                     /gene="actS"
                     /locus_tag="ACHKUB_004435"
     CDS             6023..6778
                     /gene="actS"
                     /locus_tag="ACHKUB_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO6657363.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6857..6930
                     /locus_tag="ACHKUB_004436"
     tRNA            6857..6930
                     /locus_tag="ACHKUB_004436"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6889..6891,aa:Gly,seq:ccc)
     gene            7152..>8312
                     /locus_tag="ACHKUB_004437"
     CDS             7152..>8312
                     /locus_tag="ACHKUB_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO6657364.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STPDQIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIV"
BASE COUNT         2175 a         1938 c         2071 g         2128 t
ORIGIN      
        1 cattagaaaa tgataaaaat cttatgtttt tatcaaagcc aatttcgcct gagttaatat
       61 catcatgatc tgtgaaccat caacgtcttc aatgatcgac ttttaagtaa gtctggaagg
      121 taaagctatg gagcttgcta ctacgcagtc agtattgatg caaattcaac cgacaattca
      181 gcgttttgcc agaatgcttg ccagcgtttt gcagcttgag gttgagatcg ttgatgaaaa
      241 cttgtgtcgc gtcgccggaa cgggcgcgta tgggaagttt cttggtcgcc agttgagcgg
      301 caactcacgc ctgctccgcc acgtcctgga aacgaaaact gaaaaagttg tgacacagtc
      361 tcgcttcgat cccctttgcg aaggttgcga tagtaaagaa aattgccgcg aaaaagcatt
      421 tctgggtacg cctgtcattt tacaggatcg ttgtgttggg gtgataagtt tgattgccgt
      481 tacccacgag caacaagagc atatcagtga taatttacgc gaattttctg attatgttcg
      541 ccatatatcc accatttttg tttcgaaact tctggaggat caggggccag gagataacat
      601 cagtaaaata ttcgcgacca tgatcgataa tatggatcag ggcgtattag ttgttgatga
      661 tgaaagtcgg gttcagtttg ttaatcagac tgccttaaaa acacttggtg ttgtacaaaa
      721 taatattatt gggaaaccta tccgtttcag accattaaca tttgagagta attttactca
      781 tggacatatg cagcatattg tttcgtggga cgataaaagt gaattaatca ttggtcaatt
      841 gcataacatt cagggccgac aattattttt aatggcattt caccaatcgc ataccagttt
      901 ttctgtagca aatgcacctg atgaaccaca tattgaacaa ttggttggcg agtgccgtgt
      961 tatgcggcaa ttaaaacgac tcattagccg tattgcaccc agcccatcca gcgttatggt
     1021 ggttggtgaa agcggcacgg gtaaagaagt cgtcgcccga gcaatccata agttgagcgg
     1081 aagacggaat aaacccttta ttgctatcaa ctgtgccgcg attccggagc agcttctgga
     1141 aagcgaactg ttcggttatg ttaaaggcgc atttactggc gcttctgcca acggtaaaac
     1201 agggttgatt caggcggcga atacgggcac gctgtttctc gatgaaatag gtgatatgcc
     1261 attaatgttg caggctaaat tactgcgcgc tattgaggcg cgtgaaattc tgccgattgg
     1321 tgccagtagc ccaatacaag tcgacattcg catcatttct gcaactaatc agaatttggc
     1381 ccagttcatt gccgaaggta aattccgcga agatctcttc taccgactta atgttatccc
     1441 gataactctg ccaccgctgc gtgaacgtca ggaagatatt gaactattgg tgcattactt
     1501 tttacatctg catacccgtc gtctgggatc ggtttatcct ggcattgctc ccgatgtcgt
     1561 cgaaatattg cgtaagcatc gttggcccgg aaacctgcgc gagttaagca atttgatgga
     1621 atatctggtt aacgtagttc cttcaggtga agttatcgac agcacgctat tgccgccaaa
     1681 tctgctgaat aatggcacaa cggagcaaag tgatgtaaca gaggtcagtg aggcgcacct
     1741 gtcactcgat gatgcgggcg gcacggcgct ggaggagatg gaaaagcaaa tgatccgcga
     1801 ggcgctttca cgtcataaca gcaagaagca agttgctgat gaactgggca tcggcattgc
     1861 tacgctctat cgcaagatta agaaatatga gttgttaaac acataacctc agtgaatccg
     1921 gcctgaattc aggccggata tccttttact tcgttttctc gcaatccaga actgtattta
     1981 caatcatctg ataccccgtg cagcgacaaa gatttcccgc cagtccgcga cgaatttccg
     2041 taatggttaa tggcttctcg cgtggtttcg ccagcattgc cgtggtagcc ataatcaggc
     2101 caggcgtaca aaacccgcac tgcactgcgc cggatttcgc ataagcctgc tgaacatgag
     2161 aaagttttcc gcctttcgct tcaccttcca gcgtgcggat ctcttttcct tcagcccagg
     2221 cggcaaggta taagcaactg tctattgctg tgccgtcgac caacaccgta caggcaccac
     2281 attcacccac gcagcaccct tgtttgacac ttagcagtcc ttgttcgcgg agtaattccg
     2341 agagcggcgt gcctggtgcg gcgtgaagct gaaaaggcat cccgttaatg gtgcattcga
     2401 tggtaattgt ttcgctgtga ttcattgcaa ttttcccccc gccgcggcga cggcttcgct
     2461 aatcactttt ttggtcatcg tctggatgag atgcagacga aactctttac tggcccgcca
     2521 tgaagaacgc ggggcgacat cttgcaggac agattcgctg atagcttcca gcgtttgcag
     2581 gtttaatggc gcattttgtg cagtctgttc ggcatgttgg cagcgaatcg gcgttggcgc
     2641 ggcaacacca aatgccaggc gtaattcgct gaaattgccg ttatccagtc ggcaatgtgc
     2701 ggcgcagcca atcgttgaaa tatccattgc gtcgcgcatg gcatatttaa aatgcgcgct
     2761 gcccgcgtgt tctttcggct gtggcggaaa atgaaaggcg acgaggattt cgtcatgctc
     2821 aagagacact ttgcccggcc cggtgtgaaa gccattaatc gggacgaaac gaacaccgcg
     2881 cggggagtgg atctccagtt tcgcgtcata aattagcgtt ggcgtggcag aatctgcgct
     2941 ggtggcaccg ttgcaaatat ttccaccgta ggtagcgacg ttacggatct gcggtccagc
     3001 aatggacgtg gccgcagcac ataacgccgg gagatgacgt tgagttatag gatcttctat
     3061 tagctgggta aatgtcgttg cagagccgat acgtagcgag ccatcttccg ccagcgtaat
     3121 tccccgcagc tccgccagat tatgaatatc aacaatatgg cgataacggt cattgtgatg
     3181 gtggagctga atcagtacgt cagtgccacc ggcgagcagt ttggcctgcg ggttgtcagc
     3241 cagcaggttg atggcatcgg caagggttgc tgcgcgatgg taagaagcaa aatcaaacat
     3301 gatgttatcc tcaaatcaat cctgccagat ggaactcttc atataaccgt tttggcgtca
     3361 gcggcagtgt attgattgca acaccggtag ccatcttcac cgcgttacga atagcagcgg
     3421 caacaggaat tattggtggc tcacccagtg acttatgtcc gtatgcggat tgcggctcat
     3481 tgatttcgac gaacgcgctt tccagttgtg gcagatccgg catggtcggc attttgtaat
     3541 ccagcagatt ggggttacgg accacgccgc ttttagcatc gatgatcatc tcttcaaata
     3601 gcgcccagcc aatgcccatt cccattccgc cgtgtacctg accttctgcc agcagtggat
     3661 taagaatatg ccctgaatca tgaacgttga ggatgcggtt gatgatgact ttgcacagcg
     3721 caatatcgac cgtcagatca acaaaggtac agccaaacgc cggtgggtta gtggtggttt
     3781 tgatggagct ttcagcagag agctgcccgc cgcgttcagg gtggtagaaa gcgtccatcg
     3841 ccaaatcttt taacgacatt aacggctctt ccggtcgttc aaccagcacg atatggcctt
     3901 ttatcagggt cagattcatc gctgactgat gtagcatgac tgcggcgtga gcgatgattt
     3961 tctcttttaa taatagtgcc gcactgcgca gcgcaggcgc ggcaacatag ctctggcgtg
     4021 aggcaaatgc gccgggatcg aacggcgtaa cgtcggtatc ttgagttgaa ataacgcgaa
     4081 cgtcgctgac cggaaccccc acggtttctg ccaccatttg cgagaagacg gtgtcggcac
     4141 cctgaccgat ttccgtcgcg ccgctttgca cgttgatggt tccatcctga ttcatcagaa
     4201 ggcgcgcgcc tgctatttct acgccgacag gccaggtgtt agaggtgtag ctaaaacagg
     4261 cgacgccaac gccgcggcgc aaattgcctt gctggttctg gcattctgca cggcgttttt
     4321 cccattcaaa gattttccgg cctttttcaa gacactccgg caaccctgcg ctgtaaatac
     4381 gtttgcccgt gagcggatta gcatctcctt cgcgggcggc gttgcgtaaa cgaatttcaa
     4441 caggatcaat acctaacgct gtcgcggcgt catcaagcat agactcaacg gcaaatacga
     4501 cttgtggcgc gccataacca cgcatcgcac cagccgaggg gaggttggta tagcaggtct
     4561 ttgaactgta agcgtaggca caacgaggat aaaggtaagc gactttattc ccccccgcag
     4621 aagcgatgga gtgcccgtga gatgcataag cgccggtgtt agacagaaca tccagactat
     4681 aacctttcaa tgttccgtcg cggttcacgc ccatttgccc gtcaatggta aaagcgtggc
     4741 gggtacgggt tgcgaggaaa cactcttcac ggctaaggga aactttcacc ggaatgccgc
     4801 caagcttgct ggtcaggaat gccgccattg gctcttccag tacatcctgt ttattaccaa
     4861 aaccgccacc gacaaacggt ttgatgactc gtacgcatga ccagggaata tccagcgcct
     4921 gaccaaccac gcggcgaaca atgtgcggga tctgggtgct ggaaacgatg gtaattcgcg
     4981 agtcatcctc catccacgcc agcgatgtta cgctttccat gtgacaatgt tgaataacgg
     5041 gggtctgata gtgcccctgt acctggtagt cggcggcatc gattgtttgt tggacattac
     5101 ccgtcgacat cgtgctttgt ttcagtaaat tgccaccgtt atggattggt gcagcgtctt
     5161 ctgccagcgc cgcttctggc gtggtgataa cgggtaattc ttgccactca atgctgacca
     5221 attgtgccgc tttttctgcc gtgagttcat cgcgggccac gacgatggca acggcgtcac
     5281 catgatgacg aacatggcga gttagcagtg cgcgatcggc ggtatcgcgc ttgttttcgt
     5341 caagtgtcca ggcatgccca gcagtagcaa atggaatatc aggcacatct tcccaggtaa
     5401 aaatcgccag tacgcccggc aaacttctgg cttgttcatc attaatactt acggcataac
     5461 catgtgcgat agggctacgt acatatttcg cataacacat gcccgccata acataatcgt
     5521 cagtatatcg tgcccgcccg gtgaccttag caatggcatc gacgcgcatg catgattcac
     5581 ccgtagcggt tgcttcccgc gcttccatag aaatcccctc gatttgaaca tttggttctt
     5641 atggcaaaga tttagtctaa gcaggcaaga tttacgccag aaacgacgat aagcgttagg
     5701 ggaggtagga agtgtgatga cgttaaaaaa acacagatat gatacgaaac aggactctga
     5761 ataggggcag tgatacgtat caatatgagt ggcgataaaa tcaatgtgat aaattgggat
     5821 tattacgcat caggatggga aatagggtaa aaagtcagga agttcgatac ctctacagcg
     5881 aatattttgc tgatgatact tccctttgcc aaatgaaatt aacctctttc tctattacca
     5941 cgtttttcca gaagcaagag attgggtatc aagaggctgg ctgctatgat aaggcgtctt
     6001 gttttttaag cgaggaaaga ttttgagtgc gggacgcctg aataaaaaat ctctgggtat
     6061 cgtgatgttg ttatcggttg gactgctttt ggcgggctgt tcgggtagca aatcatccga
     6121 tacaggaacg tattccggct ccgtttacac cgtgaaacgg ggggatacgc tatatcgtat
     6181 ttcgcgcacc acgggaacca gcgtaaaaga actggcgcga ctgaacggca tttccccccc
     6241 ttacaccatt gaagttggtc agaaactaaa actaggtggg gcgaaaagta gcagtagtac
     6301 acgtaaatca accgccaaat caacgaccaa aaccgcatcg gttacaccgt catcagcggt
     6361 accgaaatct tcctggccgc cagtagggca acgttgttgg ttatggccaa cgacagggaa
     6421 agttatcatg ccgtattcga cagcagatgg cggcaataaa gggattgata tctcagctcc
     6481 acggggtaca cctatttacg ccgcgggtgc aggaaaggtg gtgtatgtgg gcaaccagct
     6541 gcgtggctac ggtaatctca tcatgattaa acacagtgaa gattacatta cggcttacgc
     6601 ccataatgac acgatgctgg taaataatgg gcaaagcgtg aaggctgggc aaaaaatcgc
     6661 cactatgggg agcacggatg cggcatctgt tcgcctgcat ttccagattc gttaccgtgc
     6721 aacggcaatt gatccgctac gttacttgcc gccgcagggc agcaagccaa aatgctgatg
     6781 gcgaattaat cagcagtcag cagcgcggca cttgctaagg agagcgtaag gtttataatg
     6841 ccttacgcat ctcgaagcgg gcgtagttca atggtagaac gagagcttcc caagctctat
     6901 acgagggttc gattcccttc gcccgctcca atttatcttc acctgatcaa tctgccattt
     6961 ttgctgtatc tgattttcta tattatctta attcaatcgc ttcagacttt tcgcaatcgc
     7021 cgtaatactt ccccattttg ttgtaaataa aaagcgttta cgcaatgcaa tccttttgta
     7081 acaacccagg taaaacacct tattttcaag ttgttaaata agtggttatc tacgctttta
     7141 acaaacgatt gatggaatat tttatgaaag ggaaatctgc attaactctt ctgctcgctg
     7201 gtatattttc gtgcggcaca tgccaggcga caggggcaga agtgacgagt gaatctgtat
     7261 ttaacattct taattccacg ggggcagcaa cagataaaag ttatttatcg ttaaatccag
     7321 ataagtatcc taattatcgt cttttgattc actcagccaa attacaaaat gaaataaaga
     7381 gccactatac caaagacgaa attcaaggat tgctaacatt aacagaaaac acacgaaagt
     7441 taacgttgac ggagaaaccc tggggaacgt ttattcttgc ttctactttc gaagatgaca
     7501 aaacagcggc agaaacccac tatgatgctg tctggctgag ggatagttta tggggctata
     7561 tggcattagt atcagatcag ggaaatagtg tagccgcaaa aaaggttctg ctcaccctgt
     7621 gggattatat gtctacgccc gaccagatta agcggatgca ggacgtcatt agcaacccca
     7681 aacgactgga tggagttccc gggcaaatga atgttgtgca tatccgattt gacagcaact
     7741 cccccgtgat ggcagatgtg caggaagaag gcaagccgca gctgtggaac cataaacaaa
     7801 atgatgcgtt aggtctttat cttgatctcc ttatacaagc aatcgacact gatacaatta
     7861 acgctgaaga ttggcaaaaa ggtgaccgat taaaatcggt tgcgctgctg attgcttatc
     7921 tggataaagc caatttctat gttatggaag attccggagc atgggaagaa gatgcccgat
     7981 taaacacatc ttccgttgca ctggtgacct ctggactgga acgtttgtcg aacttactct
     8041 cgaagaaaga ctctgttttt gtctctgatt tactacgcga agctaaagcc aatgaactgg
     8101 atgaacctct ttcaacaacg aggttgaacc acctcataga taaaggttat gaaagaataa
     8161 cgttacagct tgatttaggg ggagaatccc caggttatct ggaaaaagat aaacattaca
     8221 gagaagccga tgcggcttta cttaatgtta tttatcctgc aaatcttgca aagataaaca
     8281 ccagaagaaa agagcaggtt ctaaaaatag tt
//
LOCUS       DAZFRN010000192         8253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFRN010000192 DAZFRN010000000
VERSION     DAZFRN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.194"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..497
                     /locus_tag="ACHKUB_004438"
     CDS             <1..497
                     /locus_tag="ACHKUB_004438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657365.1"
                     /translation="FRGVVITKSFQRRRGHRIWREKMINITTKDGTQVSGEVRDLVIL
                     LETDGKTVSDYLQPIPPKKNTHWSMIFIPVTIYILLNLVYIIAHPLFPEKYLKTSTSI
                     FLFISFILSAIVAYFVFHKHKSATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLD
                     SLSK"
     gene            complement(576..1289)
                     /locus_tag="ACHKUB_004439"
     CDS             complement(576..1289)
                     /locus_tag="ACHKUB_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6657366.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVMAFRENGITTMEQVNAGMRVARRQERPFLPS
                     PGQFVAWCKQSGGALGITVDQVITEYWDWRNRSFEFTSSEHFPWSQPVMYHICVELRH
                     RSTERQLTHGELAREAGDLLDMWERRVTEGKPVPPVRRAIAAPAAEHGPTPIQLLQAK
                     YNRNKSNGMVRSGKEDNKE"
     gene            complement(1286..2305)
                     /locus_tag="ACHKUB_004440"
     CDS             complement(1286..2305)
                     /locus_tag="ACHKUB_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6657367.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKDVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENALSVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVEL"
     gene            complement(2302..2841)
                     /locus_tag="ACHKUB_004441"
     CDS             complement(2302..2841)
                     /locus_tag="ACHKUB_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO6657368.1"
                     /translation="MQPLTYQQTSGFIPTAVINRSQTKQAPGHEKIRDAVRAWSAVDN
                     QDVVAALIVNEYREQGDGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(2911..3141)
                     /locus_tag="ACHKUB_004442"
     CDS             complement(2911..3141)
                     /locus_tag="ACHKUB_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6657369.1"
                     /translation="MNPAIKTAINIVGSQKKLGDACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3246..3935
                     /locus_tag="ACHKUB_004443"
     CDS             3246..3935
                     /locus_tag="ACHKUB_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6657370.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4165..4545
                     /locus_tag="ACHKUB_004444"
     CDS             4165..4545
                     /locus_tag="ACHKUB_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO6657371.1"
                     /translation="MRKILIAAMMASVLAGCASSGNQQLKNETEISVQSKLQEGKTTK
                     NEVKSYFGSPDAVSYTDSGNEIWKYAFAKVKVNGTTFIPFYGLFHNGTNGTKKELTIL
                     FNDDTIKKYTMSETQINSKSGWAD"
     gene            5026..5232
                     /locus_tag="ACHKUB_004445"
     CDS             5026..5232
                     /locus_tag="ACHKUB_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO6657372.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            5308..5604
                     /locus_tag="ACHKUB_004446"
     CDS             5308..5604
                     /locus_tag="ACHKUB_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6657373.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            5610..6395
                     /gene="bet"
                     /locus_tag="ACHKUB_004447"
     CDS             5610..6395
                     /gene="bet"
                     /locus_tag="ACHKUB_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO6657374.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            6392..7072
                     /locus_tag="ACHKUB_004448"
     CDS             6392..7072
                     /locus_tag="ACHKUB_004448"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO6657375.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQWQ"
     gene            7069..7251
                     /locus_tag="ACHKUB_004449"
     CDS             7069..7251
                     /locus_tag="ACHKUB_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO6657376.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            7224..7421
                     /locus_tag="ACHKUB_004450"
     CDS             7224..7421
                     /locus_tag="ACHKUB_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO6657377.1"
                     /translation="MHKASPIELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEAEADERDQV"
     gene            7498..7713
                     /locus_tag="ACHKUB_004451"
     CDS             7498..7713
                     /locus_tag="ACHKUB_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6657378.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            7812..8033
                     /locus_tag="ACHKUB_004452"
     CDS             7812..8033
                     /locus_tag="ACHKUB_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6657379.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELISKQRGSK"
BASE COUNT         2232 a         1946 c         1938 g         2137 t
ORIGIN      
        1 gattcagagg agtggtcatc acaaaaagct tccaaagacg tagagggcat cgtatttgga
       61 gggaaaaaat gattaacatt actactaagg atggcactca agtcagtggt gaagtaagag
      121 atctagtaat tcttcttgaa actgatggaa aaacggtgtc tgattatttg cagccaatcc
      181 ctcccaaaaa aaatacacac tggagcatga ttttcattcc ggtaacaatt tatatactac
      241 ttaatcttgt atacattatt gctcacccat tatttcctga aaaatatcta aaaacatcaa
      301 catctatatt tctattcatt tccttcatat taagtgcaat agtagcatat tttgttttcc
      361 ataagcataa atctgccact ctatccatat gcagcttagt gttcttagtg ttaattgtag
      421 gtgctaacgt agggtttatc agttacagcg acctaacaca aaaagcgacg caaaaattag
      481 atagtttgtc taaataatta tgttgccatg cgaagtatgc atggctgcat gtctgccttc
      541 catttaaaat gggctaagac ctataaccct aaatattatt ctttattatc ttctttacca
      601 cttcgcacca tcccgttcga cttgttgcgg ttgtacttcg cctgaagcag ctggattggc
      661 gtcgggccat gctcggcagc cggtgctgca atcgcccggc gcacaggcgg tactggtttg
      721 ccctcggtga cgcgcctctc ccacatgtcc agcagatcgc cggcttcacg cgccagctca
      781 ccatgcgtta actggcgctc tgtgctgcgg tggcgcagtt cgacgcagat gtggtacatg
      841 acaggctgtg accacgggaa atgctcgctg gaagtgaatt cgaaggaacg attacgccaa
      901 tcccagtatt cggttatgac ttggtcaaca gtgataccta acgccccgcc actctgctta
      961 caccacgcta cgaactggcc gggcgacggc aggaatggtc gctcctgtcg gcgggccacg
     1021 cgcattccgg cgttaacctg ctccatagtg gtaattccat tttcacggaa agccataacc
     1081 cactgacggc ggatttcgtt cagctcgttc tggtcgcggt tcgcgaggct tgccggaaat
     1141 gtcgcaagca gctggctgaa cacaccgttg atgatctgcg ccacctgctg cacctgcggc
     1201 ttttcgtcgt attgttccgg catgttgttg gcgattcgac gcatctgctc acggtcaaag
     1261 ttaatcatct gcgcggcaat atttttcata gctccacccc gtaaatccag tcagtgtttg
     1321 tcaggtcgag ttttggtttg ctggcagtca caccagcctg ttgcttgttg cggttaattt
     1381 cgagctgagt ccacttgtcg cggagttttg ctggactcag cacgttaccg gaccagaagt
     1441 tgtcctggca ggcccagcgg aacagaacgc acatgtcgcg atggttacgc ccgtcacgct
     1501 cacgcatcag gcggatgtcg ttggcccacc ctgcaaagtt tggttttctg gctgaagggg
     1561 cgatagtttt caccatgtca aacatccact ctgcggcggt caggtcttct gctgtccccc
     1621 acttgctgcc gctctgaatt gcagcatccg gtttcaccac agaaagggca ttttctggct
     1681 ggtcagagga ttcgccagaa ttctctgacg aaaaaggttt tatattgtct tttgttagtt
     1741 tgtcttttgt gtttacctga ttcgggtaaa cgtctttacc tgatttgggt aaactttttt
     1801 tacctgattc aggtaaattt acctctttca ggtaaacttt atttttctta cctgattcgg
     1861 gtaatgttga ccattcactg accacattat taatgccggt attccgcccg ctctgaataa
     1921 gaatcccacg ctttaccaga acactttttg cagcagaaca tttgtgcggc aatatcccgg
     1981 tcaattcgga cagttgctcg ttgctcaccc aatccagttt tttattaaag ccatatgttt
     2041 tgcgcatgac agctaggaag accagaagct ggtgctgtgt taatccggcc agcatcacag
     2101 cttccagcaa ctcatttgca atgcgcgtat aaccatcatc gagatctgcc acgcgcggct
     2161 ccttttgtgc cgcatccggc actggaaaat tgaatatctc agcagtgttt gccataattc
     2221 ctcccgcaat gagtgtgtta cgatttgcac ctgaaagtcg gttctgttcc agcagaccgg
     2281 ctttcgccat ttctgaacct gtcatatcgc ccccagcatg gtagtaacca tcgccatcaa
     2341 tggaccagcc agatctgggt ccacacgaaa catcgacaca ataccttcac taatttcctt
     2401 cagtttctgg tggcgtggtg cgttgagaat gacagcctgt tttgcctcac tgagttcctt
     2461 ttccatttca gccaacctag ccatgaagct atcctgctca accaggtaac cgcgatattc
     2521 cagcggtagt accgccagaa ttgccggggt cagttcacgc acgttatttc ggtatttttc
     2581 agaatcgaat ttgttatcga ggaagcggaa cagcttctgg cgtgcacggc tgacatcatc
     2641 agggaaatcg atggtgccgt cgccctgctc ccgatactca ttcacaatga gtgcggcaac
     2701 gacatcctga ttatctacag ccgaccaggc gcggacggca tcacggattt tttcgtggcc
     2761 tggagcttgt tttgtttgag aacgatttat caccgcagtc ggaataaatc cgctagtctg
     2821 ttggtatgta agtggttgca taattgactc ctttagtttg aattgactgt taagttgatt
     2881 gcttattgtt aaagagcgtg aaatggaaat ttaagctgcg ttcttttcgg tgtgtggaaa
     2941 caacttcgga agatccgggc gaatctggta tgccttcaca actccaccag tagccgtaac
     3001 aatgctgccg acatgttcag gggatacctt tgctttgttg tgaagccact tatagacggc
     3061 ctgctgtgaa acttcgcagg catcgcctag tttcttttgt gaaccaacga tattgatcgc
     3121 tgttttgatt gctgggttca taacaacctc cgtggttaat ccgaatcaag attaaaacta
     3181 tggttgtttt tagtcaacaa ccattttcgt ttgatgaaat aaaaccttgg ttgtacattt
     3241 gatctatgaa aacaacactc tcagaaagac ttaaagaagc cagattagcg cgaggcctta
     3301 cacaaaaggc gcttggggat ttggtcgggg ttagccaagc tgctattcag aaaatcgaaa
     3361 cagggaaagc taaccaaaca actaaaatcg tggagatcgc gaacgctttg ggtgtgcgcg
     3421 cagaatggtt atcttctggc gttggaaata tgtcagacag tacagtgcaa ccaatacaat
     3481 caactgtcag ccattccaaa tacttcaaaa ttgacgttct tgatatagaa gtcagtgccg
     3541 ggccaggtgt aatcaaccgt gagtttgtag aagttctacg ctcggttgag tactcgtttg
     3601 acgatgctcg tcacatgttc gatggcagga aggcggaaaa tatccgcatc attaacgtgc
     3661 gaggtgacag catgtcaggg acgatcgaac caggtgacct gctgttcgtt gatatcactg
     3721 ttaaatcttt cgacggtgat ggcatctatg cgtttctgta cgacgacact gcccatgtaa
     3781 aacgtcttca aatgatgaag gataagctgc tggttatctc tgataacaag agctactcgc
     3841 cgtgggaccc gatcgagaaa gacgagatga accgggtgtt catattcggt aaggtcattg
     3901 gaagcatgcc gcagacgtac aggaagcatg gttaatttat ctacgactta agggagcgaa
     3961 ggttaaggtt tataccactc ggattagata ttgcactaaa ttctctataa gaacgccaaa
     4021 tctgtttgca tatttcagta aatattctcg ttgttagctg agatttgttg ctactgtcag
     4081 caaaatgtcc ccctatctcg tagcggtttt tatttcgaat cattatgtta agatgtttct
     4141 gattataatg aatggaaaca taaaatgaga aaaatcctaa tcgctgccat gatggcatct
     4201 gtattggctg ggtgtgcttc ttcaggcaac cagcaactca aaaatgaaac tgaaattagt
     4261 gtccagtcta aacttcagga aggtaaaaca accaagaatg aggttaaatc ttactttggt
     4321 tctcctgatg ctgtttcata tactgacagt ggaaacgaga tctggaagta cgcctttgca
     4381 aaagtaaaag ttaatggcac cacttttatt ccattctatg gattattcca taatggaacg
     4441 aacggtacga aaaaagaact tactattctt tttaacgatg acacgattaa gaaatacaca
     4501 atgtcagaaa cccaaataaa ctcgaaatca ggttgggctg actgataatc atacccggca
     4561 accgcgccgg gttttctttt cctccccctc ataactcata ccgtccaaaa aaccaccaca
     4621 cctcacttca gttatcgcta tgcgatgcaa gtcacaaaat aaatccatcc taaatacaac
     4681 cagttatatt taaaacaacc gacaaaacaa cttttgttgt tgacgataaa acaactatag
     4741 ttttaaataa attcatcgca acgacacaac gatacggtaa ccacctgttt caccgtggcg
     4801 atgaccgctt agatccgcag tttgaatttc agcaggcttc ggggagtgcg aggggtgaaa
     4861 cggacgcgtg aacgtcggtg tgaccagctg aaatcaacac aacactttat acctcagtcg
     4921 cttcaacgag gcggcttagt tatgacaacc ggcggccatc caccgcctga atacgcgcag
     4981 aagtctctat atgttcagca gcccagctta cgggcaggag tttttatggt tcatcaacat
     5041 tacggaacgc agaccgttaa tcgcggtgcg gtcatgccag gaatgctggt caaacacaaa
     5101 gatggtacct ggactgcatc agctaattta cgcggacggc tttatctgca tcgcggcatc
     5161 gagcgcactt atacccgtga tttgctcgtg gaagtttttc tcgacggacg cggtaacggc
     5221 ctcaatcact aatccccttt cctgttttcc taatcagcct ggcatttcgc gggcgatatt
     5281 ttcacagcca ttttcaggag ttcagccatg aacgcttatt acattcagga tcgtcttgag
     5341 gctcagagct gggcgcgtca ctaccagcag atcgcccgtg aagagaaaga ggcagaactg
     5401 gcagacgaca tggaaaaagg cctgccccag cacctgtttg aatcgctatg catcgatcat
     5461 ttgcaacgcc acggggccag caaaaaagcc attacccgtg cgtttgatga cgatgttgag
     5521 tttcaggagc gcatggcaga acacatccgg tacatggttg aaaccattgc tcaccaccag
     5581 gttgatattg attcagaggt ataaaacgga tgagtacagc actcgcaacg ctggcaggga
     5641 agctggctga acgtgtcggc atggattctg tcgacccaca ggaactgatc accactcttc
     5701 gccagacggc atttaaaggt gatgccagcg atgcgcagtt catcgcattg ttgatcgtcg
     5761 ccaaccagta cggccttaat ccgtggacga aagaaattta cgccttccct gataagcaga
     5821 acggcatcgt tccggtggtg ggcgttgatg gctggtcccg catcatcaat gaaaaccagc
     5881 agtttgatgg tatggacttt gagcaggaca atgaatcatg tacatgccgg atttaccgca
     5941 aggaccgtaa tcatccgatc tgcgttaccg agtggatgga tgaatgccgc cgcgaaccat
     6001 tcaaaacccg cgaaggcaga gaaatcaccg gaccgtggca gtcgcatccc aaacggatgt
     6061 tacgtcataa agccatgatt cagtgtgccc gtctggcctt cggatttgct ggtatctatg
     6121 acaaggatga agccgagcgc attgtcgaaa ataccgcata cactgcagaa cgtcagccgg
     6181 aacgcgacat cactccggtt aacgatgaaa ccatgcagga gattaacact ctgctgatcg
     6241 ccctggataa aacatgggat gacgacttat tgccgctctg ttcccagata tttcgccgcg
     6301 acattcgcgc atcgtcagaa ctgacacagg ccgaagcagt gaaagctctt ggattcctga
     6361 aacagaaagc cactgagcag aaggtggcag catgacaccg gacattatcc tgcagcgtac
     6421 cgggatcgat gtgagagctg tcgaacaggg ggatgatgca tggcacaaat tacggctcgg
     6481 cgtcatcacc gcttcagaag ttcacaacgt gatagcaaag ccccgctcag gaaagaagtg
     6541 gcctgacatg aaaatgtcct acttccacac cctgcttgct gaggtttgca ccggtgtggc
     6601 tccggaagtt aacgctaagg ctctggcctg gggaaaacag tacgagaacg acgccagaac
     6661 cctctttgag ttcacttccg gcgttaatgt tactgaatcc ccgatcatct atcgcgacga
     6721 aagtatgcgc accgcctgct ctcccgatgg tttatgcagt gacggtaacg gccttgagct
     6781 gaaatgcccg tttacctccc gggatttcat gaagttccgg ctcggtggtt tcgaggccat
     6841 aaaatcggct tacatggccc aggtgcagta cagcatgtgg gtgacacgaa aagatgcctg
     6901 gtactttgcc aactatgacc cgcgcatgaa gcgtgaaggc ctgcattatg tcgtgattga
     6961 gcggaatgaa aagtacatgg cgagttttga cgagatggtg ccggagttca tcgaaaaaat
     7021 ggacgaggca ctggctgaaa ttggttttgt atatggggag caatggcaat gaagcatcct
     7081 cacgataata tccgggtagg cacgatcact ttcgtctact ccgttacaaa gcgaggctgg
     7141 gtatttcccg gcctttctgt tatccgaaat ccactgaaag cacagcggct ggctgaagag
     7201 ataaataata aacgaggggc tgtatgcaca aagcatctcc tattgaatta agaacgagta
     7261 tcgggatggc acatagcctc gctcaaattg gagtcaggtt tgtgccaata ccagtagaaa
     7321 cagacgaaga atttcatacg ttagcaacat ccctttcaca aaagctggaa atgatggtgg
     7381 cgaaagcaga agcagaagca gatgagagag accaggtatg acaaccactg aatgcatttt
     7441 tctggcagcg agcttcatat tctgtgtgct tatgcttgcc gacatgggac ttgttcaatg
     7501 acacctcagc aagaaaacgc ccttcgcagc attgcccgtc aggctaactc tgaaatcaaa
     7561 aaagccagac agcagtttcc ggataaaaac gtcgatgaca tttgccgtag cgtactgaag
     7621 aagcaccgcg aaacggtaac gctgatggga ttcacaccga ctcatttaag tctggcgatc
     7681 ggcatgttaa acggcgtctt taaggagcga tgaacatgaa aagcaaaatc atcagggagc
     7741 tacaggctcc ttttttatta ttcgcattca ccctcaagcg tattaaccaa caattcaggg
     7801 attaatgaaa gatggcagac ataattgatt cagcatcaga aattgaagaa ttacagcgca
     7861 acacagcaat aaaaatgcgc cgcctgaacc accaggctat atctgccact cattgttgtg
     7921 agtgtggcga tccgatagat gaacgaagac gactggccgt tcagggttgt cggacttgtg
     7981 ccagttgcca gcaagatctg gagcttatca gtaaacagag aggttcgaag tgagcgaaat
     8041 taactagaag ccaaagataa aatcatcgct gagcaggaga aaatcgctaa cggagaaaag
     8101 acagtaagtc agtatatgaa aaccgcatga tatcatcaga taaaaatcaa tcgtaaagcg
     8161 aaatattaat accagaataa acgagtcgag gtaaattata ttacctcgat aaattaacta
     8221 aaacttgccc gctatatact atctcattca gta
//
LOCUS       DAZFRN010000193         8216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFRN010000193 DAZFRN010000000
VERSION     DAZFRN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.195"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            113..631
                     /locus_tag="ACHKUB_004453"
     CDS             113..631
                     /locus_tag="ACHKUB_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO6657380.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
     gene            complement(676..2721)
                     /gene="dcp"
                     /locus_tag="ACHKUB_004454"
     CDS             complement(676..2721)
                     /gene="dcp"
                     /locus_tag="ACHKUB_004454"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO6657381.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2858..3604
                     /gene="ydfG"
                     /locus_tag="ACHKUB_004455"
     CDS             2858..3604
                     /gene="ydfG"
                     /locus_tag="ACHKUB_004455"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO6657382.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3693..4379
                     /gene="rspR"
                     /locus_tag="ACHKUB_004456"
                     /gene_synonym="ydfH"
     CDS             3693..4379
                     /gene="rspR"
                     /locus_tag="ACHKUB_004456"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO6657383.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4557..4760
                     /gene="ydfZ"
                     /locus_tag="ACHKUB_004457"
     CDS             4557..4760
                     /gene="ydfZ"
                     /locus_tag="ACHKUB_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO6657384.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4796..6256)
                     /locus_tag="ACHKUB_004458"
     CDS             complement(4796..6256)
                     /locus_tag="ACHKUB_004458"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6657385.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6345..7628)
                     /gene="ydfJ"
                     /locus_tag="ACHKUB_004459"
                     /pseudo
     CDS             complement(6345..7628)
                     /gene="ydfJ"
                     /locus_tag="ACHKUB_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT         2189 a         2084 c         1866 g         2077 t
ORIGIN      
        1 tcttaaacat attttcagaa taatcggatt tatatgtttg aaaattatta tatttgacga
       61 gcatacagaa aaagcaaatc acctttacat ataatagcgt ggacaaaaaa cagtgaacat
      121 taataaagat aaaattgtac aactcgcaga taccgacact attgaaaacc tgacatccgc
      181 gttgagtcaa agacttatcg cggatcaatt acgcttaact accgccgaat catgcaccgg
      241 cggtaagttg gctagcgccc tgtgtgcagc tgaagataca cccaaatttt acggtgcagg
      301 ctttgttact ttcaccgatc aggcaaagat gaaaatcctc agcgtaagcc agcaatctct
      361 tgaacgatat tctgcggtga gtgagaaagt ggcagcagaa atggcaacag gtgccataga
      421 gcgtgcggat gctgatgtca gtattgccat taccggctac ggcggaccgg agggcggtga
      481 agatggtacg ccagcgggta ccgtctggtt cgcgtggcat attaaaggcc agacctacac
      541 tgcggttatg cattttgctg gcgactgcga aacggtatta gctttagcgg tgaggtttgc
      601 cctcgtccag ctgctgcaat tactgctata accaggctgg cctggcgata tctccaggac
      661 agccattggt ggtgtttata tgttcaagcc acgatgttgc agcatcggca taatctgagg
      721 agccttaccg cgccattgtc gatacaggcg ttccagatct tcgctgttac ctctggaaag
      781 gatcgcctcg cgaaaacgca gcccattttc acgcgttaat ccgccctgct caacaaacca
      841 ctgataacca tcatcggcca acatttgtgt ccacagataa gcgtaataac ctgcggcata
      901 tccgccacca aaaatatggg cgaaataact gctgcgatag cgtggcggta tagcaggaag
      961 atccatattt tccgccacca gcgcccgcaa ttcaaaatca tcgacatcct gcattgcttc
     1021 gttttcttcc aggcaatgcc agcgcatatc gagaagtgcg gcgctaagca gttcgctcat
     1081 ctcataccct ttgttgaaca ggctggcatt acgcattttc tgttgcagtt cgtcaggcat
     1141 tgctgccccg ctctgataat gccgggcgta gcgagcgaat acctgcggat gcgttgccca
     1201 gtgttcgttg atttgcgacg gaaattcgac aaaatcacgc ggcgtgttgg tgccggaaag
     1261 cgtggcataa cgctggcggg caaaaaggcc gtgcagcgta tgaccaaatt catggaataa
     1321 ggttatgaca tcatcccaga gtaacaacgc aggctcaccg gcagcgggtt tctgataatt
     1381 gcaaacgtta taaattaccg gatgcgtttc attaagcgtt gattgctcaa caaaattgcc
     1441 catccatgca ccgccgcttt ttgaatcacg ggcgaagaaa tcaccgtaaa ataacgccag
     1501 tcccacgcca ttatgatcaa aaatttccca cacacgaacg tcaggatggt agacaggaat
     1561 atcaaaacgt tcgacaaact taataccgaa gagctgattc gcggtccaga atacaccttc
     1621 attcaacacc gtgtttaatt caaaatatgg cttgagctgc gcctcatcaa gatcaaattt
     1681 ctcccgccgt acctgttcgg cataaaatgc ccagtcccac ggctgcgcgc taaacccgcc
     1741 ctgctgctta tcaataaccg cctgtatgga ggctaattca tcgctcgcac gttgacgcgc
     1801 cgctggaaca atttcccgca taaagttgag cgctgcttca ggtgtttttg ccatctgatc
     1861 ggcgattttc catgcggcat aatgaggaaa accaagtaat gtcgcctgtt gtgcacggat
     1921 ctccaccaga cgttgaatga tagcgcgggt atcattgcca tcattttttt ccgctcgcgt
     1981 ccagcccgca ataaacagtt tttcacgcgt cgcacgatcg cgcatttcgg caagcgccgg
     2041 ttgttgggtg gtattcagca gcggaatcag ccatttgtta tccagacctt tctcgcgagc
     2101 cgcctctgcc gccagcgcaa tctcttgctc actcattcct gccagctgcg cgatatcgtt
     2161 cacaaccaga ccgccggatt tatttgctgc cagtaaccgc tggttaaact gactggtcag
     2221 tgtcgcagct tctgtattca gtacttttaa ttttgcttta tcagcttgcg caagtttggc
     2281 tccggcaagg acaaaacgtt gatgaatcac ctccaccagg cggatggatt cactatcaag
     2341 ccccagggat tcacggcgct gccagacagc atctacccgc gcgaataatt caccgttcag
     2401 atagatatca ttagccagtt ccgccagttc agcggaaaac tgttcatcaa gacgctgtaa
     2461 ttcatcattg gtatgcgccg cagtcatcgc aaaaaagacg ctggtaacgc gggtaagtaa
     2521 ttctccgctt tgttccagtg ccagaatagt attgttgaaa tcaggcgttt gcgggttaag
     2581 cgcgatggca gcaatttctg cccgcttttg ctgcattccc tcatcgaatg ccgggcgata
     2641 gtgatgattg gcaatttgat caaaatgagg agccagatac ggcagtgtgc tttgcacaag
     2701 gaaaggattc attgttgtca ttttcttctc ctgaacgcga ggtgttccat agcgtaggct
     2761 tactgataac ggagtgcaat cttgcaaacc agtattaccc gctcttaagc atcacgtgct
     2821 atgttattga cacacaaaag cgttgaggaa cagtgagatg atcgttttag taactggagc
     2881 aacggcaggt tttggtgaat gcattactcg tcgttttatt caacaagggc ataaagttat
     2941 cgccactggc cgtcgccagg agcggttgca ggagttaaaa gacgaactgg gagataatct
     3001 gtatatcgcc caactggacg ttcgcaaccg cgccgctatt gaagagatgc tggcatcgct
     3061 ccctgccgag tggtgcaata ttgatatcct ggtaaataat gccggcctgg cgttgggcat
     3121 ggagcctgcg cataaagcca gcgttgaaga ctgggaaacg atgattgata ccaacaacaa
     3181 aggcctggta tatatgacgc gcgccgtctt accgggtatg gttgaacgta atcatggtca
     3241 tattattaac attggctcaa cggcaggtag ctggccttat gccggtggta acgtttacgg
     3301 tgcgacgaaa gcgtttgttc gtcagtttag cctgaatctg cgtacggacc tgcatggtac
     3361 ggcggtgcgc gtcaccgaca tcgaaccggg tctggtgggt ggcaccgagt tttccaatgt
     3421 ccgctttaaa ggcgatgacg gtaaagcgga aaaaacctat caaaataccg ttgcattgac
     3481 gccagaagat gtcagcgaag ccgtctggtg ggtgtcaacg ctgcctgctc acgtcaatat
     3541 caataccctg gaaatgatgc cggttaccca aagctatgcc ggactgaatg tccaccgtca
     3601 gtaattttta tacccggcgt aactgccggg ttattgcttg tcacaaaaaa gtggtagact
     3661 catgcagtta actcactcac aagcaagaac gaatgaccgt cgaaacgcaa cttaatccca
     3721 cacagcctgt caatcagcag atttatcgta ttcttcgtcg cgacattgtc cattgcctga
     3781 ttgcaccagg cacaccgttg tcggaaaaag aagtttctgt tcgtttcaat gtgtcacgcc
     3841 agccggttcg tgaagccttt attaaactgg cggaaaacgg cctgattcaa attcggccgc
     3901 agcgtggcag ctacgtcaac aaaatttcca tggcccaggt gcgaaacggc agttttatcc
     3961 gtcaggccat tgagtgcgcg gtggcgcgtc gggcggcgag catgattacc gaaagccagt
     4021 gctaccaact ggaacaaaat cttcaccagc aacgcattgc cattgagcgc aagcaactgg
     4081 atgatttttt tgaacttgat gacaacttcc atcaactcct gacgcagata gccgactgtc
     4141 aactggcgtg ggataccatt gagaacctga aagcgaccgt tgatcgcgtg cgctatatga
     4201 gtttcgacca tgtttctcca ccagaaatgc tgttacgcca gcatcttgat attttctctg
     4261 ccctgcaaaa acgtgatggc gatgcggtag aacgtgcaat gacgcaacat ttgcaggaaa
     4321 tcagcgaatc cgtgcgccag atccgccagg aaaacagcga ctggtttagc gaagagtaat
     4381 tcatttactc tcatcccatc cgggatgaga ggcttttccc cccgccttac ggctcatgca
     4441 tgcatcaaaa aagatgtgag cttgatcaaa aacaaaaaat atttcactcg acaggagtat
     4501 ttatattgcg cccgttacgt gggcttcgac tgtaaatcag aaaggagaaa acacctatga
     4561 cgacctacga tcgtaaccgt aacgcaatca ccactggcag ccgtgttatg gttagcggca
     4621 ccggtcacac tggcaagatc ctgtcgattg atactgaagg tctgaccgct gagcaaatcc
     4681 gccgcggaaa aaccgtagtt gttgaaggtt gtgaagagaa actggcacct ctggacctga
     4741 ttcgtctcgg catgaactaa gtgtgtgaat gccgccgatg gcggcattgc tttttttact
     4801 tcacggaata tttcgccacg gtcgctttcg cgccatgcgc taataaagac aagtacgctt
     4861 ccgtcacttt tgcagtaaac aagctattgt ctggcaaatc atcaccaaag attgccttaa
     4921 tcgccagcaa tgactggacg cgcgctttcc cttcggcact actttgtaca gccttctgaa
     4981 taacaggtaa cagtgggtca ctgatttcta tcggatttcc ctgttcatca acaccaccga
     5041 cataacgcat ccaacccgcg acgcccagcg ccagcagatc gaacttgctg tcatgcgcca
     5101 gatgccagcg aacagaatcc aacatccgct gtggcaattt ctggctacca tccatcgcaa
     5161 tctgccaggt tcgatgacgt aacgccgggt tgctatagcg tgcaattaat cggttagcgt
     5221 aatcttgcaa atcaacgccc tgcactttca gcgtcggcgc ttgttcctgc aacatcaagc
     5281 catacgccgc ataacgataa tgttcatctt ccatgcagtc attaatgtgc tgatatccag
     5341 caagataacc caaatacgcc aggaatgaat gactgccgtt gagcatgcgc aacttcatct
     5401 cttcataagg cagcacatca ctaaccagtt cggctcccgc tttttcccat tctggacgtc
     5461 cggcaacaaa gttatcttct attacccact ggcggaaagg ttcacaggca acgcccgcag
     5521 gatcgcccac accggtaagt tgttcgattt tcgccagcgt atcctctgtc actgcgggca
     5581 caatacggtc caccattgtt gatgggaaag tcacgttatc ttcgatccat tgtgccagtt
     5641 tttcatcaac ggcttgcgcg taggaagtga caacgtcacg cataacatga ccgttttctg
     5701 gcatgttgtc acatgacatg acggtaaatg cgggaagtcc tgccgcttta cggcgagcca
     5761 gcgcctcaac aatcactcct gttgctgttt tcggctggtg gggattttgc acgtcggcag
     5821 cgaccatcgg gtgatcgagc attaactgtc cggtcgccgg agagtggaaa tacccttttt
     5881 cggtgattgt cagagagaca atcgcgattt gcggttcaca catcgcagcc aacacggttt
     5941 ctaagccatc catctgtacg tgcaaggctt ttttaacgac gccaacgacg cgagccgtcc
     6001 acgcatcggc cgacatttcc gcaacggtat aaagattatc ttgctgttgt aaatcggcaa
     6061 tttgctgttc gccgccgatt aagttgacct cataatatcc ccagtcactg aaatgttccg
     6121 tagcaagaat atcggcatac acgccctgat gcgcacggtg aaatgcacca aagcctaaat
     6181 gaacaattct tggggccagg ttattacgat cataaacagg gagtgtcgct tttgctgata
     6241 acaaattatt tcccataaca attccttaaa tataaatatg gcaagctaca tgttttgtta
     6301 tatgaataaa aatcccctct ccggtaagag aagggattga ggatttacag acttctggaa
     6361 ggttgcgcag ctcttacaac acgcggttga tcttccgcgg cgtcttccag cgcacttaaa
     6421 tcacggtctt tcacttctgg cattttcagc gcagagatta aaccaatcac tgaatatgcc
     6481 atgatcataa tggcgatcgg ataccaggat tccgtcatgg tgcagaaaat acccgccagg
     6541 ataggaccaa aaccggaagc gataagacca ccaatttctt tagaaatagc catccgggta
     6601 aagcggtttt tacagccgaa catttctgcc atggtaatgt tttccagagc aaataatccc
     6661 agcaccgcac agttatgaat cacaatcagt gcaaccataa tggtgctcgg ggcatagctt
     6721 ttatctacga tgatagaaag cattggccat gccagcacaa tcgcggaggt attcataata
     6781 atatacggga tccggcgacc aattttatcg gataaccaac caaggaacgg aatggtcata
     6841 aagccgagaa tcgaactgat catcaatgca tctgttggaa ttgctttgtt aaacaataac
     6901 gtctgcacta aatagcctgc aaggaaagtc tgaattaaac ctgagttacc cgcctgacca
     6961 aaacgcagcc ctgttgccag ccagaaggat ttgctctgga acatgctacc agcaggtgca
     7021 ggttttgctg tcggttggtt gctgtcatta accttctcaa agaccgggct ttctttcaga
     7081 ttcatacgca accagatagc aaagaccatc acgacaacgc tcgccaggaa cggtatacgc
     7141 catccccacg ccagcagttc ctctttactg agaatgaaga acataaaggc ccagattgcc
     7201 gttgcgctca aggttccgca gttagttccc atagctacaa atgaggagat aattccgcgc
     7261 ttaccttttg gcgcatattc cgccagcatc gtaccggcac cggaaatttc cgcacctgca
     7321 cccaacccct gaataatacg cagcgtcacc agcaagatgg gggcaaaaac accaatctgt
     7381 gcataggtcg gtaacacacc aattaaggtg gtacagatcc ccatcatggt gatggtaata
     7441 aagagcactt ttttacgccc tattctgtcg cccattttgc cgaaaataaa tgctccgaca
     7501 atacgcgcca cataacctgc accgtaggtt cccattgcca aaattaacgc cattgccgtt
     7561 gatgattcag gaaaaaatat ttcatgaaac actaacgctg cgccgagcga atataactgg
     7621 aaatccataa ttcacaggtg ttttttccca tcctgtggtt tccttggcgt tttctaggtt
     7681 ttttcagata gttgcatttt ttaaaaagca tcctaagttc gatctcagtg tctatctggg
     7741 gcctatttct gtcccatata tgccccaaaa aaactcccca acagataagt agttttttca
     7801 tggatttatg cgtaaaatca agaacggctg gaaatcattc aatactcaca ctatcgaaaa
     7861 atttaccagc caaccgcagc acgttcttgc ataaggcgtg tctgcggttt ttcaactatt
     7921 cagatacatc actcccatca cattcattcc tccgcatcaa aggcatatag gctatatcac
     7981 cttgatattt ttcttcttca gataaaaact gttatctatg tatactttta aacccaatcc
     8041 gtgtagagtc tctgcataag atagtttgca gttgccgctt cagcttgcgc cataaaccgc
     8101 ctgatttttg ctgccacctg ttagcattcc tgtatacctg aaacgacaat gtttatctac
     8161 gaactttaag aacacccaag ataaaaattg tcaactatat catatataac acatta
//
LOCUS       DAZFRN010000194         8199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFRN010000194 DAZFRN010000000
VERSION     DAZFRN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.196"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            141..5561
                     /locus_tag="ACHKUB_004460"
     CDS             141..5561
                     /locus_tag="ACHKUB_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO6657386.1"
                     /translation="MNRIYRVIWNCTLQVFQVCSELTRRVGKKSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGGNENSKGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGAGEATIREGGLITAENTIIGGNATGF
                     GTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKEYSLVGVQDGSHGVINVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMDISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNNGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGFITNATKVEFGLGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTADDMNVMPGG
                     ALRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5770..6036
                     /gene="hipB"
                     /locus_tag="ACHKUB_004461"
     CDS             5770..6036
                     /gene="hipB"
                     /locus_tag="ACHKUB_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO6657387.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6036..7358
                     /gene="hipA"
                     /locus_tag="ACHKUB_004462"
     CDS             6036..7358
                     /gene="hipA"
                     /locus_tag="ACHKUB_004462"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO6657388.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7670..7878
                     /locus_tag="ACHKUB_004463"
                     /pseudo
     CDS             7670..7878
                     /locus_tag="ACHKUB_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2240 a         1750 c         2232 g         1977 t
ORIGIN      
        1 ttagcaaatt agccaaattg acaccttccc acctccaact aacactcaat acagacctct
       61 gacaatttaa taagtttcat ttttaatgtc tggcgactca tctctttcgt ggctgtattg
      121 tttgtatatg gagcgtttat atgaatagaa tctatcgcgt gatatggaat tgcaccctac
      181 aggtatttca ggtctgctcg gaattaactc gcagggtagg taaaaaatcg acggttaatt
      241 tgcgtaaatc ctctggactg acaacgaaat tcagtagatt gacgctgggt gttttgctgg
      301 cactaagcgg ttcagcgtct ggtgcgagtc tggaagttga taatgatcag attaccaata
      361 ttgatactga tgttgcttat gatgcctacc tggttggctg gtatggcact ggagtgctta
      421 atattttggc tggcggtaat gcctccttaa ccacgattac taccagcgtc attggtggta
      481 atgagaactc gaagggcacc gttaatgttt tgggtggcac ctggcgattg tatgatagcg
      541 gaaataatgc aaggccttta aatgtgggtc aatccggaac ggggacgctg aatattaaac
      601 agaaaggtca cgttgatgga ggatatttaa ggctaggttc ctcgacagga ggcgtcggga
      661 cggtcaatgt tgagggagag gactctgttc tgacgaccga attattcgaa atagggagct
      721 atggcacagg ctcattaaat attacggata agggttacgt cacgagttca atagtcgcca
      781 ttttaggcta tcaggcgggc agtaatggcc aagttgtcgt tgaaaagggc ggtgagtggc
      841 taataaaaaa taacgattcc tcaattgaat ttcaaattgg taatcaagga gcgggggaag
      901 cgactattcg cgagggtggg ctgattacgg ctgaaaacac gattatcggt ggcaatgcca
      961 ccggtttcgg aaccctgaat gtgcaggatc aagactctgt catcacggta cgcagactct
     1021 ataatggata tttcggaaat ggcacagtca atatttccaa taatggactg attaataaca
     1081 aagaatattc attggtgggc gttcaggacg gttcccacgg tgtcatcaac gtgaccgata
     1141 aagggcattg gaatttcctc ggaacaggcg aagctttccg ctatatctat atcggtgatg
     1201 ctggcgacgg tgaacttaat gtctcgcgtg aaggtaaagt agattcggga attatcactg
     1261 cggggatgaa agaaacaggc acaggcaaca ttactgttaa ggataagaac tccgttatca
     1321 ctaatctcgg aactaatctt ggttatgacg gccacggcga aatggatatc agtaatgagg
     1381 ggcttgttgt cagcaacgga ggaagctcac tcggttatgg agaaaccggc gtcgggaatg
     1441 tcagcatcac cacggggggg atgtgggagg tcaataagaa tgtctatacc accattggtg
     1501 ttgcgggcgt cggaaacctc aatattagcg atggcggtaa gttcgtatcg caaaatatta
     1561 cttttttggg cgataaagca agcggtatcg gcacactgaa cctgatggat gcgacatcat
     1621 cgttcgatac tgtgggtatc aatgtcggta attttggtag cggtatcgta aatgtcagta
     1681 atggtgccac ccttaattca acgggctatg gatttatcgg aggaaatgcc tccggtaagg
     1741 ggatagttaa tatttcaacg gacagtctct ggaatttaaa gacatcatct actaacgcac
     1801 aattgctaca ggtcggtgta ttaggcacgg gtgaactgaa tattaccacc ggaggtatag
     1861 ttaaagcgcg tgatacacag atagctctca atgacaaaag taagggcgac gtgagggtgg
     1921 atgggcagaa ctctcttctt gaaacattca atatgtacgt agggacatct ggtacgggta
     1981 cgttaaccct gacgaataac ggtacgctga atgtcgaagg tggagaagtt tacttaggtg
     2041 tttttgagcc tgctgtagga acgctaaaca ttggtgctgc tcacggtgag gcggcggcag
     2101 atgccggatt tatcaccaat gcgacgaaag tggagtttgg tcttggcgaa ggcgtttttg
     2161 tctttaatca taccaataac agtgatgccg gctaccaggt cgatatgctg attacgggtg
     2221 acgataaaga cggaaaagtg atccatgatg caggccatac ggtgttcaat gcagggaata
     2281 cttatagcgg taaaacgctg gtcaatgacg gcctcctgac cattgcgtct catacggcag
     2341 atggggtaac gggcatgggt tcgagtgaag tcaccattgc aagccccggt acgctcgaca
     2401 ttctcgcatc aacgaacagt gcaggagatt acacgctgac caatgcgctc aaaggcgatg
     2461 gtttgatgcg agtgcagctg tcatcctccg acaagatgtt tggctttacc catgcaacag
     2521 ggactgaatt cgccggtgtt gcccaactga aagacagtac cttcactctg gaacgcgaca
     2581 acaccgctgc gcttactcac gcgatgttgc agtctgacag tgaaaatacc acatcggtaa
     2641 aagtgggaga gcaatccatt ggtggactgg ccatgaatgg cggtacgctc attttcgata
     2701 cggatattcc tgctgcgacg cttgcagagg gatatatcag cgtcgatacg ctggttgtcg
     2761 gcgcgggtga ctacacctgg aaaggccgta actatcaggt aaacgggacg ggcgacgtgc
     2821 ttatcgacgt gcctaaaccg tggaatgatc ccatggcgaa taaccctctg acgacgctca
     2881 atttgctgga acacgacgat agccatgtcg gcgttcaact ggtgaaggcg caaacggtta
     2941 ttgggtcggg tggctcatta acgttacgtg atttacaggg cgacgaggtg gaagcggaca
     3001 aaacgttaca cattgcgcaa aacggaacgg tggtcgccga gggtgattat ggattccgcc
     3061 tcacgaccgc accaggtgat ggtttgtacg ttaactatgg gctgaaagcg ctgaacatcc
     3121 atggtgggca aaagctgacg ttagccgaac atggcggagc ctatggcgca acggccgata
     3181 tgtcggcaaa aatcggtggt gaaggggatc tggcaatcaa tacggtgcga caggtttcgc
     3241 tttccaacgg tcagaacgac tatcaggggg caacctacgt tcagatgggg acattacgta
     3301 ccgatgcgga tggtgcactt ggcaacaccc gggaactgaa catcagcaac gcggccatcg
     3361 tcgatcttaa tggatcgacg cagacggtag agacattcac cgggcagatg ggttcgactg
     3421 ttttgttcaa agagggggcg ctgacggtaa ataaaggtgg gatcagtcag ggtgaactga
     3481 caggtggcgg aaacctgaat gttacagggg gaacgctggc tatcgagggg cttaatgcac
     3541 gctacaatgc gttaaccagc attagcccaa atgcggaagt cagcctcgat aatactcagg
     3601 ggttaggcag aggaaatatt gccaatgacg gtctgttaac gctaaaaaac gtgactggcg
     3661 aactgcgcaa tagcataagc gggaagggta tcgtgagcgc aaccgccagg acagatgtag
     3721 agctggatgg cgataatagc cgctttgtgg ggcaattcaa cattgataca ggcagcgcgc
     3781 tcagcgtcaa cgagcagaaa aacctgggtg atgcttccgt tatcaataat ggcctgctca
     3841 ccatctccac tgagcgtagc tgggcgatga cgcacagtat cagcggtagc ggtgatgtga
     3901 caaaactggg taccgggatc ctgactctta acaacgattc cgcggcgtat cagggtacga
     3961 cggatatcgt ggggggggaa attgctttcg gttccgactc tgccattaat atggcaagtc
     4021 aacacattaa tatccataac agcggtgtga tgtcgggaaa tgtcaccact gcagatgata
     4081 tgaacgttat gcctgggggg gcactgcgtg tcgctaaaac cactatcggc ggcaacctgg
     4141 agaatggcgg cacggttcaa atgaatagcg aaggggggaa accggggaat gtactgaccg
     4201 ttaacggcaa ctataccgga aacaatggcc tgatgacgtt caacgcgacg ctgggcggcg
     4261 ataattcgcc caccgataag atgaacgtga aaggcgatac ccaagggaac actcgcgttc
     4321 gggttgataa cattggcggc gtcggtgcac aaacggtcaa cggtattgaa ctcattgagg
     4381 ttggcggtaa ttctgcaggt aacttcgcgc tgaccaccgg aactgtcgaa gctggggctt
     4441 acgtctacac gctggctaaa gggaagggga atgacgagaa aaactggtat ctgaccagta
     4501 aatgggacgg cgtaacgcca gcggatacac ccgatcccat caataatccc cctgttgtgg
     4561 atccggaagg cccatcagtt tatcgcccgg aggccggaag ctatatcagc aacattgccg
     4621 cagccaactc gctgtttagc catcgtttac acgaccgtct gggtgagccg cagtatacag
     4681 attcactgca ttctcagggg tcggcaagca gtatgtggat gcgtcatgtc ggagggcacg
     4741 aacgttcaag ggccggtgac ggtcagctaa atactcaggc taaccgctat gtattgcagc
     4801 taggcggcga tttggcgcag tggagtagca acgcgcagga tcgctggcat cttggcgtga
     4861 tggcaggcta cgccaatcag cacagtaata ctcagagtaa tcgtgtgggt tataaatcgg
     4921 atgggcgcat cagcggttac agcgctgggc tgtacgcgac ctggtatcag aacgatgcga
     4981 ataagaccgg cgcttatgtt gacagctggg cgctgtataa ctggtttgat aacagcgtca
     5041 gttccgataa ccgttctgct gacgactatg attctcgcgg tgtgacggcc tctgttgagg
     5101 gtgggtatac ctttgaagcg ggaacattta gcggcagcga agggacgctg aatacctggt
     5161 acgtccagcc acaggcgcaa atcacctgga tgggtgtgaa agattccgac catacccgga
     5221 aagacggaac gcgcattgaa acggaaggcg acggaaatgt gcaaacgcga cttggggtga
     5281 aaacctacct gaatagccat caccagcgtg acgatggtaa acagcgtgag ttccagcctt
     5341 acattgaagc gaactggatc aacaatagca aagtctacgc cgtgaagatg aatggtcaaa
     5401 ccgtaggccg tgaaggtgcg cgtaatctcg gtgaagtacg taccggggtt gaggcgaaag
     5461 taaataacaa ccttagcctg tgggggaatg tcggtgtgca actaggtgat aaaggctata
     5521 gcgatactca gggcatgctg ggagtgaaat atagctggta aaccgtataa gccgcatgtc
     5581 gagatggcat gcggcttaat attgccgact tcaaaggctt taaagcctcc cttttatccg
     5641 cgatcgcgga tatcgcagcg tttatcccgt acagcggata agatgtgttt ccagattgac
     5701 tttatcctca ctaaaggata aaactgataa tatcccctta agcggataaa tttgctgtgg
     5761 acgtatgaca tgatgagctt tcagaagatc tatagcccaa cgcaattagc gaatgcaatg
     5821 aaactggttc gccagcaaaa tggctggacg cagagcgagc tggcgaaaaa aattggcatt
     5881 aagcaggcga caatttccaa tttcgaaaac aaccctgaca ataccacgct cacgacattt
     5941 tttaagattt tacagtcgct tgaactctca atgacactat gcgacacgaa aaatgcctcg
     6001 ccagaatcaa cagaacagca agatctggag tggtaatgcc taaacttgtc acttggatga
     6061 acaaccagcg ggtaggcgag ttaacgaagt tagccaacgg cgcgcacacc tttaagtatg
     6121 caccggagtg gttagccagc cgttatgcca gaccgttgtc actttcgctg ccattgcaga
     6181 gggggaatat cacctcagat gccgtattta acttcttcga taacctgtta cccgatagcc
     6241 cgattgtacg tgaccggatc gttaaacgtt atcatgctaa atccagacaa ccgtttgatt
     6301 tattgtcaga aatagggcga gacagcgttg gtgccgtaac gttattaccc gaaaatgaaa
     6361 ccataacgcg cccgataatg gcatgggaaa agcttactga agccaggctt gaagaagtat
     6421 taacggctta taaagcagat atccctctgg gcatgattag agaagaaaat gactttcgca
     6481 tctcggttgc tggcgcgcag gagaagacag cgctgcttag aataggcaat gactggtgca
     6541 ttccgaaagg aataacgccg acgacgcaca tcattaaatt accgattggc gaaatcaggc
     6601 agcccaatgc gacgctcgat ctcagccaaa gcgttgataa tgagtattac tgtctgctgc
     6661 tggcgaaaga acttgggttg aatgttccgg acgcagaaat cattaaagcg ggaaatgtgc
     6721 gcgcgttagc ggtcgaacgt tttgacaggc gttggaatgc tgagcgaacg gttttacttc
     6781 gcttgccaca ggaggatatg tgtcagacat tcggtttacc ttcatcggtg aaatatgaat
     6841 cagatggagg cccaggcatc gcgcggatca tggctttttt gatggggtcc agcgaggcgc
     6901 tgaaagatcg ctatgatttt atgaaattcc aggtcttcca gtggttgatt ggcgcaacgg
     6961 acggtcatgc aaaaaacttc tccgtattta ttcaggctgg cggcagttat cgactcacgc
     7021 cattttacga catcatttca gcatttccgg tccttggcgg tacgggaata cacatcagcg
     7081 atctcaaact ggcaatgggg cttaacgcat ccaaaggcaa aaaaacggca atcgataaaa
     7141 tttatccgcg acattttttg gcgacagcaa aggtgctgag attcccggaa gtgcagatgc
     7201 atgaaatcct aagtgacttt gccagaatga ttccggcagc actggataac gtgaagactt
     7261 cattaccgac agattttccg gagaacgtgg tgacggcagt tgaaaccaat gtgttgaggt
     7321 tgcatggacg gttaagccga gaatacggta gtaagtgaga tatggggttt ggtcgttgtt
     7381 aatgagcacg acaatcatga ccgccaacga ctggattctt ccttggttta tactggtgcg
     7441 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     7501 tattcgttca gggttcaaga acttaagcct ctcctctttc ataattggcc tgtgcatgga
     7561 gaaatagcct ggttattccc tgccccatgc taagagcgca gtgtctgtcg catagacact
     7621 gcatacgaaa acaccagagg gtgctgttac ttatttaagg acggatcaag cgggaatgag
     7681 tccactccgg tatcagaaat ggctacgtct caatgaagtc aggcgaccga tgctgaatga
     7741 acattacgat gtcaccacag cagcctatgc tgtcggttac gaaagctatc ccatttcagt
     7801 cgggaatatt cgcggatgtt tggagagtca cccaagagag atattaccag gttaagaaag
     7861 tctgtcggtc agctttaata aaccctgcac ttatctgttt agagcaattc ggtgttagtt
     7921 tcagcaagca aacattaacc atagctaatg atttatagcc atattaacca ttggtagccc
     7981 ttgacctttc ctgacacttc ccttgttcgc aataatattc tgtttagtca cggtttataa
     8041 ataacatgca taatgaaatc gtcatgttat ttagcgacat aaaatgtttt tgcaaatact
     8101 ctcttcaaca agtgcagcaa taaacttgtt gtgattaggt gtccttgtga tttaaagata
     8161 ttatcgttct tcgtgaaatg taataaccct ggaaactat
//
LOCUS       DAZFRN010000195         8175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFRN010000195 DAZFRN010000000
VERSION     DAZFRN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.197"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..2894
                     /gene="evgS"
                     /locus_tag="ACHKUB_004464"
     CDS             <1..2894
                     /gene="evgS"
                     /locus_tag="ACHKUB_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO6657389.1"
                     /translation="NSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWLDT
                     GNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNIIT
                     LQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVFVM
                     QKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELDAL
                     VATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPPSE
                     VLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQ
                     GDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENSDS
                     PFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYICG
                     WQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGL
                     SKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFG
                     AIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLVHI
                     DDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQG
                     DLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRLLL
                     KRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSSLP
                     IWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEA
                     LKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANILNL
                     QKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(2950..4095)
                     /gene="yfdE"
                     /locus_tag="ACHKUB_004465"
     CDS             complement(2950..4095)
                     /gene="yfdE"
                     /locus_tag="ACHKUB_004465"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO6657390.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4169..5113)
                     /gene="yfdV"
                     /locus_tag="ACHKUB_004466"
     CDS             complement(4169..5113)
                     /gene="yfdV"
                     /locus_tag="ACHKUB_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO6657391.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5183..6877)
                     /gene="oxc"
                     /locus_tag="ACHKUB_004467"
     CDS             complement(5183..6877)
                     /gene="oxc"
                     /locus_tag="ACHKUB_004467"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO6657392.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6931..>8175)
                     /gene="frc"
                     /locus_tag="ACHKUB_004468"
     CDS             complement(6931..>8175)
                     /gene="frc"
                     /locus_tag="ACHKUB_004468"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO6657393.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2392 a         1898 c         1710 g         2175 t
ORIGIN      
        1 ataactcgcc gcgtcaatat aattttttct tgaccagaaa agaatctgtc attcttaatg
       61 aagtactcaa tagatttgtt gatgctttaa caaatgaagt tcgctatgaa gtatcacaaa
      121 attggcttga tacaggaaac ctggcctttc tgaacaaacc attagaactc actgaacatg
      181 aaaaacagtg gattaagcag catcccaatt taaaggtgct ggaaaatcct tactcgcccc
      241 cctattctat gacggatgaa aatggctcgg ttcggggcgt tatgggggac attcttaata
      301 ttattacctt gcaaacaggt ttaaattttt ctccgatcac cgtttcacac aatatccatg
      361 ctggaacaca gcttagcccc ggaggatggg atataatacc tggcgctatt tatagtgaag
      421 atcgagaaaa taatgtttta tttgctgaag ccttcataac aacgccttac gtttttgtca
      481 tgcaaaaagc gcctgacagt gaacaaacat taaaaaaagg aatgaaagtt gccattccat
      541 attattatga gctgcattcg caattaaaag agatgtatcc ggaggttgaa tggatacagg
      601 tcgataatgc cagcgctgca tttcacaagg ttaaggaagg tgaacttgat gctctggtcg
      661 cgacacagtt aaattcgcgt tacatgatcg atcattacta tcctaatgaa ctttatcatt
      721 ttcttattcc tggcgttccg aatgcatcgc tttcgttcgc ttttcctcgc ggagaaccgg
      781 aacttaagga tattattaat aaagcactga atgcaattcc cccaagcgaa gttctgcgcc
      841 tgacggaaaa atggattaaa atgcccaatg tgaccattga cacatgggac ctatatagcg
      901 agcaatttta tattgttacg acattatccg ttttattagt tggcagtagc cttttatggg
      961 gattctacct gttacgctca gttcgtcgtc gtaaagtcat tcagggtgat ttagaaaacc
     1021 aaatatcatt ccgaaaagca ctctcggatt ccttaccgaa tccaacttat gttgtaaact
     1081 ggcaaggtaa tgtcattagt cataatagtg cttttgaaca ttatttcact gcggattact
     1141 acaaaaatgc aatgttacca ttagaaaaca gtgactcacc ctttaaagat gttttttcta
     1201 atgcgcatga agtcacagca gaaacgaaag aaaatcgaac aatatacaca caggtatttg
     1261 aaattgataa tggcatcgag aaaagatgca ttaatcactg gcatacatta tgcaatcttc
     1321 ctgcaagtga caatgcagta tatatttgtg gttggcaaga tattactgaa acgcgtgatc
     1381 taattaatgc actcgaggta gaaaaaaata aagcgataaa ggctaccgta gcaaaaagtc
     1441 agtttctggc aacgatgagt cacgaaataa gaacaccaat aagctctatt atgggcttcc
     1501 tggaacttct gtcgggttct ggtcttagca aggagcaacg ggtggaggcg atttcacttg
     1561 cctacgccac cggacaatca ctcctcggct taattggtga aatccttgat gtcgacaaaa
     1621 ttgaatcggg taactatcaa cttcaaccac aatgggtcga tatccctact ttagtccaga
     1681 acacttgtca ctctttcggt gcgattgctg caagcaaatc catcgcatta agttgcagca
     1741 gtacatttcc tgatcattac ctggttaaga tcgatcctca ggcgtttaag caggtcttat
     1801 caaatttact gagcaatgct ctcaaattta ccaccgaggg ggcagtaaaa attacgacct
     1861 ccctggttca cattgatgac aaccacgctg ttatcaaaat gacgattatg gattctggaa
     1921 gtggattatc gcaggaagaa caacaacaac tctttaaacg ctacagccaa acaagtgcag
     1981 gtcgccagca aacaggatct ggcttaggct taatgatctg caaagaatta attaaaaata
     2041 tgcagggcga tttgtcatta gaaagtcatc caggcatagg aacaacattt acgatcacaa
     2101 tcccggtaga aattagccag caagtggcga ctgtcgaggc aaaagcagaa caccccatca
     2161 cactacctga aaagttgagc atattaatcg cggatgatca tccgaccaac aggctattac
     2221 tcaaacgcca gctaaatcta ttaggatatg atgttgatga agccactgat ggtgtgcaag
     2281 cgctacacaa agtcagtatg caacattatg atctgcttat tactgacgtt aatatgccga
     2341 atatggatgg ttttgagttg actcgcaaac tccgtgagca aaattcttcc ttacccatct
     2401 gggggcttac agccaacgca caggctaacg aacgtgaaaa agggttaagt tgcggcatga
     2461 acttatgttt gttcaaaccg ttgaccctgg atgtactgaa aacacattta agtcagttac
     2521 accaggttgc gcatattgca cctcagtatc gccaccttga tatcgaagcc ctgaaaaata
     2581 atacggcgaa cgatctacaa ctgatgcagg agattctcat gactttccag catgaaacgc
     2641 ataaagatct acccgctgcg tttcaagcac tagaagctgg cgataacaga actttccatc
     2701 agtgtattca tcgcatccac ggtgcggcta acatcctgaa tttgcaaaag ttgattaata
     2761 ttagccatca gttagaaata acacctgttt cagatgacag taagcctgaa attcttcagt
     2821 tgctgaactc tgtaaaagaa cacattgcag agctggacca ggagattgct gttttctgtc
     2881 agaaaaatga ctaaatagcg gctcccacaa tgttcaaatg tgggagctat ttaccagcac
     2941 atcttacatt tatgatgaga actcctggcg aatttgttcc ccatgctggt cgagcgttgc
     3001 cgctcccggc ataacatgcg ggtccgcgca gccgctgatt tttatcggat tacccggcat
     3061 cattattccc ccggcttcaa tcaacatatt tctcgcctga gtttgtggca atttaatggc
     3121 ctcagccaca cttaataacg gcgcgacggg tacaccaact tcatgtattc tggctaacca
     3181 aacttcagct gcctgcgttt ttaacgtccg ctcaatatat tgtttaagaa tagcctggtt
     3241 ttgtacgcgt aaaatattgc tgctaaatcg gggatcatta accagttccg taagctccag
     3301 tgcctggcat aacgcagaaa aaagcttgtc attaccacaa caaatcgtaa tcggcttatc
     3361 ctgagtattg aaaacatcaa aaggtgccat gtagggatgg cgatttccca gacgttgtgg
     3421 tgacttccct gtcgcgatat atgccatcag accatgctcc agaaaactca gcgtggcatc
     3481 aaacatcgct atatcgacat gcgcccctct ctggctcttt tcgcggccat aaagtgcact
     3541 cactattccg ctgaataaat agacaccgcc gcatagatcc gcaagagatg taccaacgcg
     3601 cactggcgga gcatcaggat atcctgtttc catcattatc ccgctcattg cctgaatgat
     3661 ggtatcgtag gcaggagcat cttttagcgg accggtatgt ccgaaacctg acgatgaagc
     3721 atatatgagg cgcgggttga tttcttgaag cgtttcccat gaaaacccca gtttttccat
     3781 tgtacctggg cgaaaattct cagctaatac atcagcttgt ttgagcatat ttataaatat
     3841 acttttatcg tgatcattct ttaaatcaag aaccacactc tctttgccat gattaataaa
     3901 actgtaatag agtgactgtc catccacata gggaccaaat gtgcgggtat catcaccatg
     3961 acccggcggc tcaactttaa ttacccttgc gcccatatta caaagaagtt gagttccgaa
     4021 aggtccatta aggacatgtg tcatatcgat aactaataag ccttcaaacg gccctttgct
     4081 ttcattattt gtcatttgct tatgccttga aaaaagccga aggtgaaaag acacttcggc
     4141 atttatgtaa aatcaataac atgattaatt atgaaaccag tcgactgaca taaatccaca
     4201 agggagccgt gacgacaaaa cccagtacgc tcacagccaa tgacgctgta ccagtgcggg
     4261 tgtagacatt aaaccggctg gcaataatga ttccggagaa tgccggcggt aatgcgcctg
     4321 ccagtaccat catctgcaaa tgttcgctgt tcaaatgaca tgccatacca acgagaagca
     4381 gcgccagtgg catcagaatc agcttcagga aggtgttata agcaatttcg gcactgaact
     4441 cgaatttatg tgcagccaga gtcaaccccg cagcgaatac cgctacccct gagttagcct
     4501 tcgcaatcag attaaaggtt gggtcccatg ctgccggaat ttttacccca accaacacca
     4561 ggatcgttgc cagaacaggt gcccatacaa ctggctcctt tgccgcagaa attaatgcgc
     4621 tcagattact attcttctta ccatccgctc ctgaggaagg attcagcaaa tacagaccaa
     4681 taggaatagt aattgcgtta acaataatag aaataattgc taccactaaa cctgttgata
     4741 cggaatcacc ataaatagga tcgagaactg caaaccccag gaatccaatg gtaggtgaac
     4801 ccgcaattaa tgcacataca gctgcttctg catgggtacg tttaaaaaat ttgtagcaac
     4861 caaaccagga gaaaaagaaa catccgacaa taaccacaag tgatacaagg gtcagacgag
     4921 tgtccgcaaa aatcatttcc ctgtttgccc gggtaataga aacaaataga gccgcaggaa
     4981 gagcgtagtt taataccagt ttattaaatg cgcgggcttg atcttctgaa aaagtttctc
     5041 gtctgccgct aaaataaccc aacagcatga ttacgataat aggcaataaa tcgccaataa
     5101 aaaatgttag cataatagta tttccgataa gggtcccctc accatcgcat aatgagttgg
     5161 caagaggaga cgatgtcagt atttaattac cagcgacttg ttttgggtta agtttggtaa
     5221 tatggccact ttcagttcct gctgcagggt cgatgaccac attaataatg gtcggtttgc
     5281 gcgactgaat accggtggtt aaagcatgac gaagttcatc tgtcgtggtg acgttatagc
     5341 caacgccacg aaacgcatcc attaatttgt cataccttgc atggtgcaac agatccgttg
     5401 gtgatggtgc accagcgcca ctgagatcaa caccgtctcc tctgtagatg ccgccattat
     5461 taaaaataac gatcgtcacc ggcaggttat atcgacaaat cgtttcaatt tccatcccac
     5521 tgaaaccaaa agcactatca ccttcaatgg cgacaaccgg agaaccagaa gtcacgctag
     5581 caccgatggc atagcccata ccgatgccca tgacacccca ggtaccacaa tccagacgac
     5641 gacgtggttt atacatatca ataatatttc gtgcattatc cagggtattt gcaccttcat
     5701 taactaaata aatatcctgg ttctcgcgca atacgtcgcg cacagcactt aatgcattaa
     5761 agtaatttaa tggttgagta tctgtactta atttttcatg cattttttgt gcattttgct
     5821 gcttgtggat atttaaaata tcgcgccata ccagtggagt cgtaaatgtg ttttgtttca
     5881 gttctgccag cataccttgc atactggatg caatatcacc aacgactggc acagcaatgg
     5941 ggcggttgct gtcaatttcc tgcggttcaa tatccagttg aataaactgt gtatctgccg
     6001 cccatccttt tttaccgtgt gccaataacc aattcagtcg tgcaccaaca agcatgacaa
     6061 cgtcagcatt tgccagggca aacgaacgcg cagctgccgc agaaagtgga tgcgtatctt
     6121 caaggatccc tttcgccata gacattggca ggaatggaat ctgagtactt tcaataaatt
     6181 cacgaagctg ttcatcagct tgtgaatacg ccgcgccttt gccaaggata attaatggcc
     6241 gttcagcttt tgctaaaagc gaaattgcgc tagtgactga cttcgggcat ggcaataatg
     6301 ctggcgacgg attttcaact ttaacaatcg tggttaacgc ttcgtctttt tccatcgtcg
     6361 cggccaggac atttgctggc aaatcaagat aaactccgcc agggcgaccc gatacagaga
     6421 cccggatagc gcgtgccaat gcaatgccaa gatcctgcgg ctgattaacg cgaaatgctg
     6481 ctttggcata cggttttgcc gcattcattt ggtccagctc ttcataatca ccttgctgta
     6541 ggtcgacgat cgcgcggtcg ctggagccgc taatcatgat catcggaaaa ccatttaccg
     6601 ttgcgttggc caatgcggtc aaaccattga ggaatcctgg cgcagaaact gtcaggcaga
     6661 tccccggttt ttgggtaaga aaaccgcttg ccgcagcggc atagcctgcc gactgctcat
     6721 gacgaaaacc aatataacga atgccttccg cctgggcatg gcgtgccata tccgtcacag
     6781 gaatacctac aacaccataa atagtgtcaa tattattctg ttttaatgct tcaacgatga
     6841 tatgcatacc atctgtcatt tgaagttgat ctgacatgat taataacctc gaaattttta
     6901 taaattggga gccagaagcc cccgttaata tcagatggcg tggttttgct tcattgcagc
     6961 aatttcatcg tcgctataac ctagctcctg caatacagca gcggtatgtt cacctaatag
     7021 cggcgcagct ttaatatccg gcgtaaaggc agagaatttc attggacaac caacggtcag
     7081 atattttcca cgcaacggtt gttccacttc aacaacactg ccactttggc gcaaagaggg
     7141 atcaagtgaa atttctttca tacttaaaac cggtgcacaa ggaatatcaa actgagtcaa
     7201 ataggccacc gcttcatgtt tatcaatagt gacagtgtat ttttcgattt cagcaaaaat
     7261 atcgaaaata tgtggctgtc gtgcatgggc tgtactgtat gccggatcgg taatccattc
     7321 tggtttgccg atggctttac aggtgttttc ccagttttgc tcctgaatag tgaaataaat
     7381 ataggcgtta ggatcggttt cccagccttt acatttcagg atccagccag gctgaccgcc
     7441 accacctgca ttaccaccgc ggggaactgc atcaccaaat gtaccattcg gatactgcgg
     7501 gtattcttcc agataaccca atttatcgag acgctgctgg tcacgtaatt tcacgcggca
     7561 aaggttcaat acggcatcct gcattgacat ggtgactcgt tgcccacgcc ccgttttttc
     7621 gcgatgcagc aaagcagcaa gtaaaccgat cagcaaatgc attccggtgt tgctgtcacc
     7681 caacgctgca gcgcttacca gcggcggacc atcccaaaaa cccgtagtgg atgccgcgcc
     7741 acccgctgcc tgagcaacgt tttcataggc ttttacattc acataaggcg aacactcatc
     7801 aaaccctttg atcgaaccaa aaatcagacg tggattgatt tcttgaatat gctcccaggt
     7861 gaagcccatg tgatcaatgg cccctggatg aaagttctcg actaagatat cagcttcgcg
     7921 gatcagcttt tccattacct ctttgccttc cgctgttttg gtatttaact caatagaacg
     7981 tttgttactg ttaagcatgg tgaagtaaag cgcatcgata tcaggaatat ctcgcagctg
     8041 gtgacgcgtt acgtcaccaa cgccgggacg ttcaatttta ataacgtcag cgccaaacca
     8101 ggccagcatt tgagtacaag atgggccaga ttgcacaccg gtgaaatcga gaactttaat
     8161 tccttgaagt ggagt
//
LOCUS       DAZFRN010000196         8085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFRN010000196 DAZFRN010000000
VERSION     DAZFRN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.198"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..234
                     /locus_tag="ACHKUB_004469"
     CDS             <1..234
                     /locus_tag="ACHKUB_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657394.1"
                     /translation="VDDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLT
                     QETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            457..954
                     /gene="ubiC"
                     /locus_tag="ACHKUB_004470"
     CDS             457..954
                     /gene="ubiC"
                     /locus_tag="ACHKUB_004470"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO6657395.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            967..1839
                     /gene="ubiA"
                     /locus_tag="ACHKUB_004471"
     CDS             967..1839
                     /gene="ubiA"
                     /locus_tag="ACHKUB_004471"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO6657396.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1994..4417)
                     /gene="plsB"
                     /locus_tag="ACHKUB_004472"
     CDS             complement(1994..4417)
                     /gene="plsB"
                     /locus_tag="ACHKUB_004472"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO6657397.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4588..4956
                     /gene="dgkA"
                     /locus_tag="ACHKUB_004473"
     CDS             4588..4956
                     /gene="dgkA"
                     /locus_tag="ACHKUB_004473"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO6657398.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5066..5674
                     /gene="lexA"
                     /locus_tag="ACHKUB_004474"
     CDS             5066..5674
                     /gene="lexA"
                     /locus_tag="ACHKUB_004474"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO6657399.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5747..7072
                     /gene="dinF"
                     /locus_tag="ACHKUB_004475"
     CDS             5747..7072
                     /gene="dinF"
                     /locus_tag="ACHKUB_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO6657400.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFATT"
     gene            7188..7397
                     /gene="yjbJ"
                     /locus_tag="ACHKUB_004476"
     CDS             7188..7397
                     /gene="yjbJ"
                     /locus_tag="ACHKUB_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO6657401.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7439..7954)
                     /gene="zur"
                     /locus_tag="ACHKUB_004477"
     CDS             complement(7439..7954)
                     /gene="zur"
                     /locus_tag="ACHKUB_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO6657402.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1809 a         1985 c         2231 g         2060 t
ORIGIN      
        1 gtcgatgatg ggccagttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct
       61 acaaaagata ccaattggaa ttccacgtgt atctataaat ccagacataa gatgttgcct
      121 attaatctta ctcaggaaac actttttagc tccaaatctc atggtaaata tgcgcttttc
      181 ccaattttta ctgcgagttg gcgagctact cgtataaaga atataggtat ttaagtaaag
      241 gaaaacatca ccgttcctgg catcatgggc ggtcatgccc ctacggttgc cctcgccagc
      301 acgggcatcg gcaaagcgta aggttcaaca gcggtttacc acttcatgcg attgttgcgt
      361 ttttgttgcg cattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga
      421 tacaatgcct ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa
      481 ctgcgtgcgc tgcgctattt taaagagatc cctgccctgg atccgcaact gctcgactgg
      541 ctgttgctgg aggattccat gacaaaacgt tttgaacagc agggaaaaac ggtaagcgtg
      601 acgatgatcc gcgaagggtt tgtcgagcag aatgaaatcc ccgaagaact gccgctgctg
      661 ccgaaagagt ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg
      721 cttgccggtc gtaccgtcgt tcctgtgtca acgttaagcg ggccggagct ggcgttacaa
      781 aaattgggta aaacgccgtt aggacgctat ctgttcacat catcgacatt aacccgggac
      841 tttattgaga taggccgtga tgccgggctg tgggggcgac gttcccgcct gcgattaagc
      901 ggtaaaccgc tgttgttaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa
      961 aaaaatatgg agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt
     1021 acggataagc caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg
     1081 gcgacaccgg gcgttcctca gctctggatc ctggcggtat ttgtcgcggg tgtctggctg
     1141 atgcgcgctg ccggatgtgt ggtgaatgat tatgctgacc gaaagtttga tggtcatgtt
     1201 aagcgcacgg cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg
     1261 ctgtttgtag tgctggtact gatttcgttt ttactggtac tgacgctgaa tacgatgacc
     1321 attctgttgt cgattgccgc tctggcgttg gcgtgggtgt acccgtttat gaagcggtat
     1381 acccatctcc cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt
     1441 gccgctgtga gtgagtcggt gccattgagt tgctggttaa tgttcctcgc caatattctc
     1501 tgggcggtgg cttacgacac gcagtatgcg atggttgacc gcgatgatga tgtgaagatt
     1561 ggcattaaat ccacggcaat cctgttcggc caatacgata aattgattat tggtattttg
     1621 cagattggcg tactggcgct gatggcgatc atcggtgagt taaatggctt aggctgggga
     1681 tattactggt caattctggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc
     1741 aaccgcgagc gtgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta
     1801 ctgtttttag ggctggcaat gagttactgg catttctgat gatgtaaaaa agccggatga
     1861 tcatccggct ttcttctggg gtgcctgatg cgctgcgctt ctcaggccta cacaacagat
     1921 cgcaatttat tgaatttgca gattatggta agccggataa ggcgtttgcg ccgcatccgg
     1981 caattctcac tgattaccct tcgccctgcg tcgcactctc aatcgtcaaa cgcacgtctg
     2041 atgtaatcaa ctccgccagc aactgataaa ccttcatcgt ttctgccggt tcggcatcgc
     2101 cgctatcgct gatataccct tcatcacgca gcgtcagcac cagagaactg aacaccgcct
     2161 tgtcgaagaa ctccggcgcg ttgataccgt gcagcacgga gagacgttgc gcgacggtgc
     2221 ggctctcttt ctccagcgta ccgcggttga tcgacgggtt ggcgctcaac agccagaagg
     2281 tgatggcata acgttgcagc gtttcgcgcg cgcctgcggc gagcagctgt agcgtgcgag
     2341 aatgcgccgg gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca
     2401 tctcatttgc cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca
     2461 gctccgcttt cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga
     2521 tgtggcgatg ctgggtgaca attgccgcca tcagcgaagg cagcaccaac atatgcgcaa
     2581 tgttgttgcg atagtaggtc atcaacaccg cttgctcgcg cggcagaatg atgatgtcgc
     2641 cgattgtgtc tttctcgact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc
     2701 tggcgctggc tgaaggaacg gtagagtccg tggagtaagg tacgttgcgc atcagatcca
     2761 ggtagcagtt gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgacgcca
     2821 gaagcgcagt acagcacagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca
     2881 tcagatcggc agcaatatta ttgaccgtcg gcgttaacca ggccggacgc accgcttcga
     2941 tgggatcgat agattcacgc cagtctggta cgtgctggtt aaggtaggtc atcaacggca
     3001 ttggttcacc gaagttgacg taaccctgac cgagattacg cagcttgctt aaaccgcgca
     3061 gcatctgcgg caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac
     3121 ccacttccat gacgtgctca taaccgatat agatcggaat cagcgtaatc ggacgcgtgc
     3181 cgccacgcag catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac
     3241 gccccgtgcg ggaacgaccg ccttccacga agtactcgac ggaataacca cggctgaaca
     3301 gttcgccgag atactcacgg aaaacggtgg aataaagttt attgccttta aaagtacggc
     3361 gaataaagaa cgcccccaga cggcggaaaa tcggcccggc cggccagaag ttcaggttga
     3421 tcccggcggc gatatgcggc ggcaccagcc cctggtggta cagcacgtaa gaaagtagca
     3481 ggtagtccat gtgactgcgg tggcaaggca catacaccag ctcatgaccg tcgtgggcca
     3541 gctggcgaac gcgctcggcg ttatggacgt tgatgccctg ataaagtcgg ttccaggtga
     3601 agcccagaat gcggtcagtc aggcggatca tctcgtaaga gaaattcgcc gcaatctctt
     3661 ccatcagcgc aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt
     3721 catcttctac cgctttggca atggcgcggg aggcgagcag cttattaaac agatcctgac
     3781 gagcaggcag acgtgggccc acagcagcca gacgttgacg ggcaaagtgc atacgcgcca
     3841 cgcgcgccag tttctgtgcg atagttttat ccgtgccgtg ttcatccgcc atacggcgca
     3901 gcgaaactga cggcgagaaa cgcacaaaac tgtcgcgacc gagccacagt acggcgaaaa
     3961 atttctgtac gccgttaagc atacgcagcg gcgggtttac ttcgcctttt tcacgccccg
     4021 gcgcgcgacc aaacatcacc gatactggca ccatctgcac atccagattt gggttgctac
     4081 ggtgcaaatc gagatagtcg tggaacagct taatagactc ttctttcggc gtgtaatagg
     4141 tgaatacacg cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt
     4201 ccagcggctc taacgggtca ggcaagtcat gagccagaca ctgggcgcgt aacgtcagca
     4261 aatctgcttt cgagttgtac ggtaaaacgt acataattgg acgagaagta tccagcccca
     4321 gttccggggc aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat
     4381 tcagtaattt gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta
     4441 ataatgcaaa tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc
     4501 atgtttcgcg cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga
     4561 cgctcataat gtaaaaaggt tctttcaatg gccaataata ccactggatt cacccgaatt
     4621 attaaagctg ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca
     4681 ttccgtcagg aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg
     4741 gacgcaatta cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc
     4801 ctcaatagcg ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc
     4861 ggacgcgcaa aagatatggg atccgccgcg gtgctgattg ccattatcgt cgccgtgatt
     4921 acctggtgca ttctgttatg gtcgcatttt ggataaccct tccagatttc gataaatctc
     4981 tggtttattg tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagcata
     5041 actgtatata cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt
     5101 tgatctcatc cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc
     5161 gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg
     5221 caaaggcgtt attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga
     5281 ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcaaca
     5341 gcatattgaa ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct
     5401 gctgcgcgtc agcgggatgt cgatgaaaga tatcggcatt atggatggcg acttgctggc
     5461 agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga
     5521 agttaccgtt aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag
     5581 cgagtttaaa ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc
     5641 ggttggggtt attcgcaacg gcgactggct gtaacatatc tctgagaccg cgatgccgcc
     5701 aggcgtcgcg gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac
     5761 ttcatctgat aaagcactct ggcatctcgc cttacccatg attttctcca atatcaccgt
     5821 tccgttgctg ggactggtcg atacggcggt aattggtcat cttgatagcc cggtttattt
     5881 gggcggcgtg gcggttggtg caacggcgac cagctttctc tttatgctgt tgctgttttt
     5941 acgcatgagc accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt
     6001 agcccgtgcg ctggtgcaac cgttgctgtt ggcgttgggg gctggggcgt taattgcgct
     6061 gctgcgtaca ccgattatcg atctggcgct gcatattgtt ggcggtagtg aagcggtcct
     6121 ggaacaggcg cggcgctttc ttgaaatccg ctggttaagc gctccggcgt cgctggcgaa
     6181 tctggtatta ctcggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt
     6241 agtggtcggc aatatcctca acattgtgct ggatgtctgg ctggtgatgg ggctgcatat
     6301 gaacgtgcag ggcgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgattgg
     6361 tctgctaatg gtgcgtaaaa tcctcaaact acgcggaatt tccggcgaaa tgctgaaaac
     6421 tgcctggcga ggaaacttcc gtcgcttgct ggcgcttaac cgcgatatca tgctgcgttc
     6481 gctgttgttg caactctgtt tcggcgcgat caccgtactt ggtgcgcgac tggggagtga
     6541 cattatcgct gttaacgcgg ttctgatgac gctactcacc tttaccgcct atgcgcttga
     6601 tggttttgcc tacgcggttg aagcgcactc cggtcaggca tacggtgcgc gcgacggtag
     6661 ccagttgctg gatgtatggc gggcagcgtg ccgccagtcg gggattgtag cgttactgtt
     6721 ttcggtggtt tatttgctgg caggggaaca catcattgcg ttgctgacgt cgttaaccca
     6781 gattcagcag ctggctgacc gctatcttat ctggcaggtg attttgccgt tggttggcgt
     6841 ctggtgttat ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa
     6901 cagtatggcg gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg
     6961 taatcatggt ttgtggctgg cattaaccgt ctttctggcg ttgcgcgggc tttctctggc
     7021 ggctatctgg cggcgtcact ggcgcaacgg tacctggttt gccacaacgt gacggttaaa
     7081 aattctgaat aaattatcct aagccaaatt gctgactaca cttaatctca cgttcagaag
     7141 aaaagtgaac gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg
     7201 aagccggcgg taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga
     7261 ccgatgatga tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg
     7321 aacgttatgg ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca
     7381 atgaatatcg ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt
     7441 aacgcggttt ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac
     7501 acgcttccac ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca
     7561 gggcaaaccc catttttgcc gccagcgtat gcataatgtc ttccacgcct tctgcacact
     7621 cttctttcac cgcgccgcag cgatcgcaaa taaacatggc tgacgtatgc gtgggctggt
     7681 cgaacagatg acagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct
     7741 caagcagaaa atccagcgcg cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc
     7801 gcagcaaatc aagcagatca taagcgctga tagcgccatc ctgcagactc atcaggcgca
     7861 acacttccag gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttttcagcct
     7921 gcgctaataa ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga
     7981 atgtccttac tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgccgtaatg
     8041 ttgattttct gttttgtacg taaggtgttg tgttgcttgg tcgta
//
LOCUS       DAZFRN010000197         8026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFRN010000197 DAZFRN010000000
VERSION     DAZFRN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.199"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(93..380)
                     /gene="yebO"
                     /locus_tag="ACHKUB_004478"
     CDS             complement(93..380)
                     /gene="yebO"
                     /locus_tag="ACHKUB_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO6657403.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(455..598)
                     /gene="mgrB"
                     /locus_tag="ACHKUB_004479"
     CDS             complement(455..598)
                     /gene="mgrB"
                     /locus_tag="ACHKUB_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO6657404.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            757..996
                     /gene="yobH"
                     /locus_tag="ACHKUB_004480"
     CDS             757..996
                     /gene="yobH"
                     /locus_tag="ACHKUB_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO6657405.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="ACHKUB_004481"
     CDS             complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="ACHKUB_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO6657406.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2107..3480
                     /gene="yebQ"
                     /locus_tag="ACHKUB_004482"
     CDS             2107..3480
                     /gene="yebQ"
                     /locus_tag="ACHKUB_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6657407.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSTPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3526..4407)
                     /gene="htpX"
                     /locus_tag="ACHKUB_004483"
     CDS             complement(3526..4407)
                     /gene="htpX"
                     /locus_tag="ACHKUB_004483"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO6657408.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4599..6647)
                     /gene="prc"
                     /locus_tag="ACHKUB_004484"
     CDS             complement(4599..6647)
                     /gene="prc"
                     /locus_tag="ACHKUB_004484"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO6657409.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6667..7365)
                     /gene="proQ"
                     /locus_tag="ACHKUB_004485"
     CDS             complement(6667..7365)
                     /gene="proQ"
                     /locus_tag="ACHKUB_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO6657410.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7462..7959)
                     /gene="msrC"
                     /locus_tag="ACHKUB_004486"
     CDS             complement(7462..7959)
                     /gene="msrC"
                     /locus_tag="ACHKUB_004486"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO6657411.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
BASE COUNT         1906 a         2126 c         1830 g         2164 t
ORIGIN      
        1 ttaatttgaa gcagttgcac catggcagtg cgtataatca ggatgaattg agtaaccctt
       61 caacgacggg gttactcaag attgaggaag gattatcgtt cggcgaccag acgaataata
      121 tcttcgtctt caaccgattt ttgactctca acggtctttt tatctgcttt ctcaggttcg
      181 tttgcttcgc acaaacgacg taacagtgca ttttgacgtt tttgctgatc cagcaacgcc
      241 tcaagcagtt caatctgttc gttagtccga gaactggcac gattgataaa aaaccacaag
      301 atgagcccga taagaagaac caccaccgat acaaccaaag acgcaatgtt catcacgcct
      361 gaatttacaa cttcgttcat ttcaccacct ctgagtagag gcgctattct accactgctg
      421 gagaggaaga aaatctagtg ctgaaaaaat gatatcacca cgggataaac tggttaatgg
      481 cacaaattcc gctgaaaaat tgtacatcct gatcgcacat catgttgaat acctgcgccc
      541 aaagcagcaa gcaagccaac accacgacaa ccagaacgac ccatcgaaac tttttcactc
      601 caatctccgc ttcacctacc ttatgtcatt aaactagcat gcgttagtta aacagcgcct
      661 aactatgtcg atattcgtgc tttttcggaa tgagtagctt gcttcagcgc atattgccgc
      721 tacgattaag cgaacataaa aagagaagag gttgtaatgc gattcatcat tcgcacagtt
      781 atgctgatag cactggtatg gataggatta ttactcagcg gatatggcgt tttgattgga
      841 agcaaagaga atgccgcagg attaggtttg caatgtacct atctgactgc cagaggcacc
      901 agcacggtac aatacttgca cactaaaagt ggtttcttgg ggataacaga ttgcccactg
      961 ttacgtaaaa gcaatatcgt ggtcgataat ggttaagggt aataaacaac cagattctca
     1021 ctcatgagta aaatattctt atagcaagcg cgtttaatat aacgcacctt caactttatt
     1081 tttctcatgc taaataaaga acctgtctgt ttgatattcc gacaggttct tactctcatc
     1141 agaacggata gtcgtgataa cccatttggg cagaaatttt gcgcgctgcg gtatgcaaca
     1201 ttgcgacata ttcctgtaaa cgctcttcag agaaacgcaa cgtcgggaag gagatgctca
     1261 aaccggcaat gaccacgcca aagcgatcaa ataccggtac cgcaatgcat cgcagccctt
     1321 cttcctgctc ttcgttatct tcgccatacc cctgctcgcg cacctggtcc agaacgggta
     1381 ataacgcttc tgtactggtg atggtccgct cggtactgcg tttatactcc acgccctcaa
     1441 gaatttgctt cacttcatcg agatcgcgcc atgccagcag taccttacca atcgcggtgc
     1501 tgtacagcgg attacgacgc ccaatccgtg aatacatgcg caaattgtac atagagtcaa
     1561 ttttgtgaat gtaaacaata ctgtcttcgt ccagtgcgcc gaggtggata gtttctttgg
     1621 tcaggcggga gatctcacgc atctggatat ctgcgctacg aattaaatcg acgttttgta
     1681 acgcgcgagc gcccagttca aacaatttca gggtcaggga atatttctcc gactcccctt
     1741 cctgcgccac ataacctaag gttttcatgg tctgtaaaaa gcgataaacg gtgctttttg
     1801 acatcatgac gcgctgcgac agctcggtta tccctatttc gcgctcttca cccagcgcct
     1861 gcaaaatgcc aaaaactttt agcacggaag atacagaatc aggctgttta tccagatctg
     1921 cgttagccat ttatcacctc attgcgagtg ttttataaaa atcagaactg ttttttatta
     1981 taatttcgca ccagggtggt cgcaatccat cttttgccgg ttagttacaa ttctgcgaca
     2041 tccaccgtga atatcagtgc tagaatcata cccctgttga ttattcacca aagatataaa
     2101 attcctatgc caaaagttca ggccgacggc ctgccattgc cccagcgata cggtgcgata
     2161 ttaaccattg tgattggtat ttcgatggct gtccttgacg gcgcaatcgc caacgtcgcc
     2221 ctgccaacaa tcgccacgga ccttcatgcc acgccagcca gttccatctg ggtagtgaac
     2281 gcctatcaaa tcgccattgt catctccctg ctctcgtttt cgtttctggg cgatatgttt
     2341 ggctatcgac gtatttataa atgcggtctg gtcgtttttc tgttgtcttc actgttctgc
     2401 gccctttctg attcgctgca aatgctcacc cttgcgcgtg tcatacaagg tttcggcggt
     2461 gcagcgttga tgagcgttaa taccgcactt atccgcctga tctatccaca acgttttctg
     2521 gggagaggga tgggcataaa ctcgtttatt gttgccgtct cttctgctgc cgggccgaca
     2581 attgctgcag caatcctctc catcgcatcc tggaaatggt tatttttaat caacgtaccg
     2641 ttaggtatta tcgccctgct tctggcgatg cgttttctgc cacccaatgg ttctcgcgcc
     2701 agtaaacccc gtttcgacct gcccagcgcc gtgatgaacg cgttaacctt cggcctgctt
     2761 atcactgcgt tgagtggttt cgctcagggg caatcgctga cgttaattgc tgcggaactg
     2821 gtggtaatgg ttgttgttgg tattttcttt attcgccgcc agctttctct tcccgtaccg
     2881 ctgctaccgg tggatttact gcgtatcccg ctgttttcac tttctatttg cacatctgtt
     2941 tgctctttct gcgcacaaat gctggcaatg gtttccctgc ccttttacct gcaaaccgtg
     3001 ctcgggcgta gtgaagtcga aacaggttta cttctgacac cgtggccgtt agcaacgatg
     3061 gtgatggctc cgttggcagg ctatttgatt gaacgcgtac atgcaggatt gctgggtgct
     3121 ctagggttgt tcatcatggc tgcggggctt ttttccctgg ttctgctgcc cgcgtcacct
     3181 gcggatatca atattatctg gccgatgatc ttatgtggcg ctggatttgg cttgttccag
     3241 tcacccaata accacaccat tattacctcc actcctcgcg aacgtagcgg tggagccagt
     3301 ggcatgttag gaacggctcg tctactgggt cagagtagcg gcgcggcact ggtggcgctg
     3361 atgctaaatc agttcggtga taatggtacg cacgtttcgc tgatggctgc ggctattctg
     3421 gcagtgattg ctgcctgtgt cagtggttta cgtatcactc agccacgatc cagggcataa
     3481 aaaaagcgcg tcgatcagga cgcgcttttt agtatttact tcatattact tcaggtattc
     3541 acccgtacgc agagcttcaa ttcgtttatc cagcggcggg tgagtcatga acaactcact
     3601 gagcgatttc gacttaccgt taatgcagaa agccatcatg ctggttgctt cttgcggttc
     3661 atagctggtt ttcaggcgct gcagcgcggc aatcattttc tcgcgaccaa ccagttttgc
     3721 cgaaccggca tcagcatgga attcacgatg acgcgagaac cacatggtga taatgctcgc
     3781 cagaatacca aacaccagtt ccagaaccgt tgcaaccgca aagtagatca gcgggttgcc
     3841 gttgctctct tcaccttcat cacgatttcc gcccataaaa cccgcggcaa gctgcgccag
     3901 aatacgggaa ataaagataa cgaaggtgtt caccacgccc tgaatcagcg tcatggtgac
     3961 catatcacca ttggcgatgt ggctgatttc gtgagcaatt accgcctcgg cttcatccgg
     4021 gctcatgttc tgcagcaaac cggtgctgac agcaaccaga gaggcatcac ggcgcgcacc
     4081 ggttgcaaaa gcgttgatgt ccggcgcatg gtagatagcc acttgcggca tagcgatccc
     4141 tgcctgacga gcctgggttg ctacagtatt aaccagccaa cgttcccttt cgttacgcgg
     4201 ttgctcgatc acttccccgc caacagatcg taatgccatc catttggaca tcagaagcga
     4261 aacgaaggaa ccaccaaaac cgaacagcaa ggccatgatc atcagcccct gaacgctgct
     4321 cgactgtatc cctgtcaggc tcagtaccag cccgaaaacg accattacgg ccaggttcgt
     4381 tagcaggaag agcgcgattc gcatcataat tttcttttta cctcagttta acaaaacgca
     4441 atatgcgata cccacatcgt atgggttacg cgactatttt caagtctgga tagtgcgtaa
     4501 gtcaccagaa agacacaact ttacattttg tagcatctga tttacggcat cttgccgctg
     4561 ttaaaaaatc aggcacaatt tcttgtgcct gattgatatt acttgacggg agcgggttgt
     4621 tccgcgggtc tggctttttc aagcttcgcc agatcgagtg cgatattcac cgtctcatcc
     4681 agataaggat ccggctcctg gtaatctttc ggtagatcat ccagcttctt caactccggt
     4741 ttaccttcgc gtttaaagcg ttcgttcaaa cgcgccagac gcgtcgcatc atcttcatta
     4801 ttctctttct cacgcacagc gtagttcaga gaaacgatat tgcgcttgtc cttcatagcg
     4861 ttgaagcgcg cgatatcctt catgatgttc tggaactcag gatctttcgc gatacgcgca
     4921 ttatgttcct tcagcagctc cggcccaaag gccgttaaat cgcctgattt cacataagtc
     4981 gcggcatcaa tgctatccca cggcagcgcg ttatcttcga atttctcacc cgtttccgtt
     5041 tcttcattac ccgtcggcat gatgatgtct ggcgttacgc ctttacgttg cgtactgccg
     5101 ccgttaacgc gatagaattt ctggatcgtg tactgcacag aacccagcgc tggccattca
     5161 ggacgtaaca tctgatcgta aatacggttc aatgaacggt attgctgaac ggtgccttta
     5221 ccaaacgtcg gttcacccac aaccagcgca cgaccgtaat cctgcattgc cgcggcaaag
     5281 atttctgaag ccgaagcact gaaacggtca accagtacca ccagcgggcc tttatagaac
     5341 acctgcccgt cggtatcgct gtcttcacga accttgccgt tgttatcgcg gacctgaaca
     5401 atgggacccg caggaataaa cagacctgag agcgataccg cttcggtcag cgccccaccg
     5461 ccattgctac gcaggtcgat gatcacactg ctgacattct gtttttccag tttctgcagt
     5521 tgaactttga catcgtctgt caaacccaca tagaagcccg gaatatccag cacgccgact
     5581 ttctctttac cgacggtctt caccgacatt ttaaccgcgc ggtcttcgag acgaatacgt
     5641 tcacgggtca atgttacagt gcgggtcttg gtccctttac cagcaggtaa aatttccaga
     5701 cgaactttac tgcccttcgg ccctttaatt aaggcaacca catcatcaag acgccagcca
     5761 atcacgtcaa ccatcggctt gcctgtttga ccaacaccga caattttgtc accaacgctg
     5821 atagctttac tcttcgctgc cggaccacct gccaccatcg aattgataac ggtgtagtca
     5881 tcatccattt gcagcactgc gccaatacct tccagcgaca aactcatttc agtgttgaac
     5941 tgttcggtat tacgcgggga aagatagttg gtatgcgggt cgatttcacg cgcaaacgcc
     6001 gtcattgcca gcgagaaaac atcttcgctg ttggtttgcg ccagacgacg aatggcaaat
     6061 ttgtagcggc gtgtcagggt ttcacgaatt tctttatccg tttttcctgc cagcttcagg
     6121 cttaactcgt cgaatttgac tttactgtcc cacagcgcgt tcaactcagc ctcgtttttc
     6181 ggccagggcg ctttgctgcg gtcaaggtta taagtgtcgt tgccggtgaa atccatcggc
     6241 ttttccagta ccgacaaagc gtactggtaa cgctcaaaac ggcgcttttg cgccagattg
     6301 tagagatcgt agaaaacgtc gagtttgcct gaacgcagtt catcgcctaa ctcggttttc
     6361 tttttcgcga actgttcaac atcgcttgcc agcagcacgt tgtggctgta atcgagcaga
     6421 ttcaggtagc ggtcaaagat tttggccgaa aatgcctgat cgaggtcgaa ctggcgataa
     6481 tgagaacggg tgaagcgcga cgttacgcgc tcacttaccg tcgcatgctg cgtctcttcc
     6541 tttaataccg gaatttgatc agcacgcgtg atatcttcta cagcgaaggt ctggcctgct
     6601 attgcaagca ggccagctaa cgcggtaagc ctaaaaaaca tgttcatgcc tggcccggcc
     6661 tccgtttcag aacaccaggt gttctgcgcg cacaatcaaa gacatacccg aattcagctg
     6721 gacgcggacg ccgtctttgg tgatttctaa tacggtggca tccatcgcgt tttgacccgc
     6781 tttcaccttc agggcttgtc cgacagtcag agctgaaatg tcagaaaccg gggtgtgctg
     6841 ttcttcgcga ggtgctttta ctgtttttgg cgctttctct accggctttt gcgcacgagg
     6901 tttacgttca gcgccttctt tgcggcgtgg cgtggtcgga cgtggcttgc gttcgcggcg
     6961 cggtgcgtct tctttctcac cagcagctgc ggcagcttcg cgttttttcg cttgctgttc
     7021 agcacgctgt gcctgaacac gcgctttcgc ttcttcaagc tgcttgcgag catgctctac
     7081 atgttgctcg tccagctcac cgcatgggtt gccgtcaaga tcgacacgcg ttgcgccggg
     7141 tttaacaccg taaagataac gccagctcga agtgtagaga cgtaaagcgg atcgtaattg
     7201 cgttttgctc aggttcattt ccccggcaac acgatcgacc aaatcctgaa aaataccgat
     7261 tttcagcgga cgcgcttcac cttccgcact gaaacagtgg ggaaaacgtt cggccagaaa
     7321 cgcgattact tctttactgc tattcaactt aggttgattt tccatgaaat ttcctgatta
     7381 caacggacgt agccaacaag cgcaggcatg aacaggcgtc attataatga cgctaccagt
     7441 aaatgctacg ttatccgttg attatcctgc gacgctcgca aagaattttt tgtaatccgt
     7501 cgttgcaagc actttttcaa gctgtgccac aagctgacgt aagccttgct cgtcctcgtc
     7561 tgtaaagcga ccgaagacgg tactatcgat gtcaagaaca ccaataatct gatttttcac
     7621 caccagcggc agaacaattt cagaattact cgccgcatca caggcaatat gcccgtcaaa
     7681 cgcgtgaaca tcctcgatac gctgcacttg attgcgggca accgcagtgc cgcacacccc
     7741 gcgcccgacg ggtatccgga cacaggcaat tttgccctga aatggtccta gtaccagtgt
     7801 atcgtcctca agcaaataaa aacctgccca gtttacgtca gtgagacgct cgtataacaa
     7861 cgcactggtg ttcgcaagcg ttgccagaaa actggtttct cccgccatca gcgcgttaaa
     7921 gtcgcgattt aaatccgcgt aaaattctgt tttgttcatt atataatcac ttggttgtct
     7981 tacctggaac tgccagccta ttaaaataag cattaaatgc gttaat
//
LOCUS       DAZFRN010000198         7877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFRN010000198 DAZFRN010000000
VERSION     DAZFRN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.200"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            524..679
                     /gene="hokD"
                     /locus_tag="ACHKUB_004487"
     CDS             524..679
                     /gene="hokD"
                     /locus_tag="ACHKUB_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6657412.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            896..1147
                     /gene="rem"
                     /locus_tag="ACHKUB_004488"
     CDS             896..1147
                     /gene="rem"
                     /locus_tag="ACHKUB_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO6657413.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            1214..1492
                     /locus_tag="ACHKUB_004489"
     CDS             1214..1492
                     /locus_tag="ACHKUB_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657414.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSDRPPRASRTFQQRIWR"
     gene            1494..2543
                     /gene="ydfU"
                     /locus_tag="ACHKUB_004490"
     CDS             1494..2543
                     /gene="ydfU"
                     /locus_tag="ACHKUB_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6657415.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSRAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            2556..2930
                     /locus_tag="ACHKUB_004491"
     CDS             2556..2930
                     /locus_tag="ACHKUB_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6657416.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRMGVKIYPIMHEEQVKK"
     gene            2927..3748
                     /locus_tag="ACHKUB_004492"
     CDS             2927..3748
                     /locus_tag="ACHKUB_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6657417.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALRRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            3892..3967
                     /locus_tag="ACHKUB_004493"
     tRNA            3892..3967
                     /locus_tag="ACHKUB_004493"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3925..3927,aa:Ile,seq:cat)
     gene            4627..4758
                     /locus_tag="ACHKUB_004494"
     CDS             4627..4758
                     /locus_tag="ACHKUB_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6657418.1"
                     /translation="MIAYCFCEWHCSSRIISVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(4852..4983)
                     /locus_tag="ACHKUB_004495"
                     /pseudo
     CDS             complement(4852..4983)
                     /locus_tag="ACHKUB_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5293..5553
                     /gene="tolA"
                     /locus_tag="ACHKUB_004496"
                     /pseudo
     CDS             5293..5553
                     /gene="tolA"
                     /locus_tag="ACHKUB_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
     gene            5725..6099
                     /locus_tag="ACHKUB_004497"
     CDS             5725..6099
                     /locus_tag="ACHKUB_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO6657419.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
     gene            6351..6566
                     /gene="essD"
                     /locus_tag="ACHKUB_004498"
     CDS             6351..6566
                     /gene="essD"
                     /locus_tag="ACHKUB_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO6657420.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            6571..6882
                     /gene="ydfR"
                     /locus_tag="ACHKUB_004499"
     CDS             6571..6882
                     /gene="ydfR"
                     /locus_tag="ACHKUB_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO6657421.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFGLDVAMDR
                     VEGAALEFYEVAARMSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            6879..7412
                     /gene="rrrQ"
                     /locus_tag="ACHKUB_004500"
     CDS             6879..7412
                     /gene="rrrQ"
                     /locus_tag="ACHKUB_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6657422.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKNVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            7409..>7877
                     /locus_tag="ACHKUB_004501"
     CDS             7409..>7877
                     /locus_tag="ACHKUB_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO6657423.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRSISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQRIYE"
BASE COUNT         2095 a         1644 c         2081 g         2057 t
ORIGIN      
        1 gagaatattg atcaagactt cataaataat agtgttgtgc ttgagcttgg ggaggacgat
       61 aagtttttta ggctttaaga tttgtaattt gatagatatg tagatatcat ttttctcgct
      121 caaaactgac agtcagattt gagcgaattg cttatgtaag tatttcgcag aatcatgcca
      181 tttatattta ttagaacata cgtactttaa atgtgggagg ctgtcatgtt ggttcttaaa
      241 tgttcgctga ctattgcggt ggtgcgggta atttattgtt ctgttattct tatggatcgc
      301 ctttctgact gatttcatat tggcgagata acgggagtta agtagaatgc ctgcgggtgc
      361 ttgaggctat ctgcctcggg catgaatacc aacggcagat agagaaaagc cccagttaac
      421 attacgcgtc ctgcaagacg cttaacatta atctgaggcc aatttcatgc tagacacatg
      481 taggttagcc tcttacgtgc cgaaaggcaa ggagaagcag gctatgaagc agcaaaaggc
      541 gatgttaatc gccctgatcg tcatctgttt aaccgtcata gtgacggcac tggtaacgag
      601 gaaagacctc tgcgaggtac gaatccgaac cggccagacg gaggtcgctg tcttcacagc
      661 ttacgaacct gaggagtaag agacccggcg agggagaaat ccctcgccac ctctgatgtg
      721 gcaggcatcc tcaacgcacc cgcacttaac ccgcttcggc gggtttttgt ttttattttc
      781 aacgcgtttg aagttctgga cggtgccgga atagaatcaa aaatacttaa gtagcgcgca
      841 gggataagag ggatggtccc ttaaagggga gagctaatta tccggaagga ttctgatgat
      901 gaacatcgaa gaactgcgta aaattttttg tgaagatggc ctctatgctg tgtgcgttga
      961 aaatggaaat cttgttagtc attaccgcat tatgtgtttg cgaaagaatg gggctgcgtt
     1021 aattaatttt gtggatgctc gggtcacgga cggatttatc ttgcgcgaag gtgagtttgt
     1081 cacttcatta caggcattga aagagatcgg aataaaagct ggcttttctg ctttttcagg
     1141 agaataaact catctacaat cttgcgcggg gctgaactcc cgctgagtaa caccgtgcca
     1201 ccggagaaaa ccgatggcac gcaacgcaaa atattacaat tctgataatt cgcccgttct
     1261 tgcctgcacg cacgggcggt attctcacgc attcaagtct gaatggttcc agcaccctcc
     1321 atgcactgca gaacaggccg aatggctgat tcattcttac cgcaggcgcg ggttcgaggt
     1381 taagaaagct ctcagtctcg actatcggca ctggataatc tctgtcaggc tgccttattc
     1441 cgaccgccca ccacgtgcgt cccgcacttt ccagcaacgg atctggaggt aacgtgcggg
     1501 tattacttag acctgttctg gtgcctgagc ttgggctggt ggtccttaag ccgggccgtg
     1561 aatccataca gatatttcat aatcctcgag tgctggtgga accggaacca aaaagcatgc
     1621 gtaatctgcc atccggagtc gttcctgccg ttcgccagcc gctggcggaa gacaaaacat
     1681 tgctgccgtt ttttagtaac gaacgggtga ttcgtgctgc tggcggcgtt ggcgcattgt
     1741 ccgactggct attacgtcat gttacatcct gccagtggcc taatggcgat taccatcaca
     1801 ctgaaacagt cattcaccgt tatggtaccg gcgcaatggt gttgtgctgg cactgcgaca
     1861 accaactgcg tgaccagaca tcggaatcac tggagctgct tgctcaacaa aatctgacag
     1921 catgggtgat tgacgtcatc cgtcacgcaa taagcggtac gcaggagcgt gaattatctc
     1981 gggctgaatt atcctggtgg gcggtccgca atcaggtggc ggacgcgcta ccggaagcgg
     2041 tattacgtcg ttcgctgggg ttgcgtgcgg aaaaaatccg ctcaatgtac cgtgaaagcg
     2101 acatcgtacc gggagagcag accgccacca gcatactgaa acagcgcaca aaaaatcttg
     2161 cgccgctgcc tcacgcccac cagcaacaga acccaccaca ggaaaagacg gtggtcagca
     2221 ttgccgttga tcctgagtct ccggaatctt ttatgaaacg acctaaacgt cgccgctggg
     2281 ttaacgagaa atacacacgc tgggtgaaga cacagccgtg tgcgtgttgt ggtaagccag
     2341 ccgacgatcc ccatcacctg attggtcatg gtcagggcgg aatggggaca aaatctcacg
     2401 atattttcac gctaccgctg tgtcgggagc atcacaacga gcttcatgcg gatccgctgg
     2461 cgttcgaaga aaagcatggt tctcaggttg atttaatttt tcgttttctt gatcacgcct
     2521 ttgcaactgg cgtacttggg taaaagaggt gactgatgct catagatttg gttttacctt
     2581 acccgccgac ggtgaacact tactggcgac gccgtggcag cacatatttt atctcggagg
     2641 agggaaagcg ttatcgccgg gctgtggcgc ttattgttcg ccagcagcgg ctgaaattaa
     2701 gcctgtccgg aaggctggcg ataaaggtga ttgcagagcc accggataag cgtcgtcgcg
     2761 acctggacaa tatcctgaaa gcaccgctgg atgcgctgac gcatgcggga gtgttaatgg
     2821 acgatgagca gtttgatgaa atcaatatcg ttcgtggtca gccagtatct ggtggacgta
     2881 tgggggtgaa gatttacccc ataatgcatg aagagcaggt caaaaaatga aactggaaga
     2941 tttaccgaaa tactactccc caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc
     3001 aaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc
     3061 tgagatgggt ttttctgcgt tcctggggaa aatgggcatc agtatgaatg acagggcgcg
     3121 ggcaacagaa ttactggcag attatgcact ccgtcggtgc gatcgtgtgg cggcgttgag
     3181 aaaacttccg gcagaaataa aaccggtagt gatgcgcatt atggcttcgt acgcttttga
     3241 ggattatgcc cgcagcgcag cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt
     3301 tattgaaagc atagttttta caaacaaggt ccagtatccg gatggtaagc cgccggtatg
     3361 ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga
     3421 ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa
     3481 ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc
     3541 tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac catcaacgga
     3601 ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat gggaaaaaac
     3661 agttaagaaa ttctatgatg cgctggtgac ccggtttgat attgaagaag catgggctga
     3721 gcggcagtta aaaaaggtaa ctaggtaaca aggttgattt ttccggaatc tgtggtaaat
     3781 tcgtcataat gatgggcttt ttatgcctga cgttagaaga gtttctacaa cccgccgccg
     3841 agcgggtttt ttattgcgga attaattacg gaccgttatt attctgctcc cggcccttta
     3901 gctcagtggt gagagcgagc gactcataat cgccaggtcg ctggttcaaa tccagcaagg
     3961 gccaccatca caaaccgcca ttagcttatc aggaagagca gacgacacga taacagggtt
     4021 gttggtgcgg gggcgggtcc ccgatggcgg tcgttgttag ctcagccgga cagagcaatt
     4081 gccttctaag caatcggtca ctggttcgaa tccagtacag cgcgccatat tcattcttcc
     4141 agattccttc cggcagagcc ttatactgga atatacctgg ctcaggatat tgttgaaaac
     4201 ataatatgtt tgtcaaaaat aaaagttctg ttaagtattg attgagtgtt tgttatacgg
     4261 tctaatggtt ttttcagcat taaatattta tcattcatat ggtgtgggta gagtgaatat
     4321 tgatgaggcg tcggggtgtt tcatccttag gcagcgtatt gatatagtca atgcagcacg
     4381 agcaaaggcc ttcagccgtt tgacagtttt gttctgtgct cctgatcgtc tttcgggaag
     4441 agacgttatt attctgaata gtgatgctat acagagggtt tgcgatgagt tcatggttgc
     4501 taattcagaa ttatttgctc ttgttcagga gtacaacaga atagccagga cctgtggtat
     4561 ggatgaactt cggattactc atctggggta gatacatatc tggattatca cctgttacgg
     4621 taaaaagtga ttgcttactg tttttgtgaa tggcattgca gcagccggat aatatcagtg
     4681 ctggctgacg gtgtgctggt ggcgggtgtg gtggttgttg ctttcccgtt gctgaaaaag
     4741 aaaacgccag actgttagcc gggtatcagt tagcgggaga aatttttaaa tacttcacaa
     4801 ttcaggcggt tgactgttct ctggtttgcg gggagtttgt taaaagaaac tggcatggtg
     4861 aatccccctg tgcggagggg caatcagcga gtaggtatat gggataatcg cggattcagg
     4921 tgctggtact gaattcaccg ggaggcaccc ggcaccatgc acttcaatag attctctcca
     4981 cattatggat attctttcag aatatcccac gcagactttg tgtaaatgtt aacaaatgtg
     5041 cgttttattt gatctgattc gctgtttgag cgtccaaaac aacggtatat ataatccttt
     5101 actatatgac atatgtgagg aaaaatgggt tttcgtagcg catcaattct tacgttgatt
     5161 attagtggga ttattatcgg gtgcactgat gctgtatcga caaattatca tgaccgtaca
     5221 tcatattact ccgataaagc aatagagaca cagtatgtga gttcatctga acgtacttct
     5281 gatgttagtg aggatatccg tctgtatgcc catcaaatca agagcgccat cgaaaaacag
     5341 ttcggggatg cgagtaagta ttcaggaaaa gagtgtacac tgagaatgca tatggccccg
     5401 aatggccttc tactggaggt taaaagagaa agtggagacc tcgatttatg tcgtgaagcg
     5461 atgaatgcga taaagaatgc tgatatacct gccccccctt cgccggaagt atataaagta
     5521 tttcaaaatg gggtgctgga ttttaaaccc tgatatttat tgttttgtaa taaacggttt
     5581 cggcttaggt ttgttctgac acaactacgg cactgagcta aatttagcgg atagtcagct
     5641 ctgagccagt ggcggacgta acaactacta ttgctgagat tttaatggat tgaggagcaa
     5701 gaagtgggat taaagaaaat cgttatgttg actttttggg tcggttttgt tgcgggatgc
     5761 acacctttac acccttcaga ttgccacaaa actactgcta caggtagttg cagttcagga
     5821 cgctgggatg atcaggatga atggggggcg caagcgcggg gaatcagagc tgcaattaat
     5881 gccaaacttg atgagccgca taactggaaa gggaaaaaat gcaggttgca tatggaattc
     5941 tctcaggatg gcacggcgtt aaaaatatct accagtaacg gtgataaagc ctattgcgaa
     6001 gcgataaagt ccgcagctca taaagccaaa tttccggcct tcaacaatcc ggaagtctac
     6061 agagattttc agaaatctgg ctttgacatg cgaggttagc tcttcaatta ctatatctca
     6121 ttcatagcaa actgacagat ttgatgatgt tctatatacg aaacctgtga tgtcaagtct
     6181 gagctaatac aaataaacat aatatcagag aaatacattt tattagctcg ctacggcgag
     6241 ctttttatat tgcatcgtct ccagcatata tatcaattaa ggctctgatt gatgtgtctg
     6301 gaagcctaca cataataact atgccatccg ttccgtgcgg aggtgaggct atgaaatcca
     6361 tggacaaaat ttcaacaggc attgcctacg gcacctccgc aggcagtgct ggctactggt
     6421 ttttacagct gctcgataaa gtcacgccct cacagtgggc agcaataggt gtgctgggta
     6481 gcctggtatt tggcctgctg acgtacctga caaatcttta tttcaagatt aaagaagaca
     6541 ggcgtaaggc tgcgagagga gagtaatcca atgactcaag actatgaact ggttgtgaaa
     6601 ggagtccgta attttgagaa taaagttacg gtaactgtag ccttacagga caaagaacgc
     6661 tttgacggtg aaatttttgg cctggatgtc gccatggacc gtgttgaagg agctgcgctg
     6721 gagttttatg aggtagcagc cagaatgagc gtccggcaag tcttcctgga agtagcagaa
     6781 aaattgtcag aaaaagttga gtcttatctg cagcatcagt actcctttaa gattgaaaat
     6841 cctgccaata agcacgagcg tcctcatcat aaatatctat gaacacaaaa atcagatacg
     6901 gcctgtcggc tgccgttctg gcgctgattg gtgctggcgc atctgctcct cagatacttg
     6961 accagtttct ggacgaaaaa gaaggtaacc acacaatggc ataccgcgat ggttctggca
     7021 tatggaccat ctgtcggggt gccacagtgg tggatggaaa aaacgttttt cccaatatga
     7081 aactgtcgaa ggaaaaatgc gaccaggtca acgccattga gcgtgataag gcgctggcat
     7141 gggtggagcg caatataaaa gttccactga ccgagccaca gaaagccggt atcgcgtcat
     7201 tctgtcccta taacattggc cctggtaagt gttttccgtc gacgttttat aagcggctta
     7261 atgccggtga tcgtaaaggc gcatgcgagg cgattcgctg gtggataaag gacggtggac
     7321 gtgattgccg cattcgttca aataattgtt acggtcaggt tattcgtcgt gaccaggaga
     7381 gtgcattaac ctgctggggg atagaacagt gaatcagata ttcacggtga ttttgctcgt
     7441 gttggtagga tttgtcgtag gtaatgtctg gagcgacaga ggatggcaaa aaaaatgggc
     7501 ggagcgtgat gctgccgaat tatctcaaga ggtaaatgtc caatttgctg ctcgaataat
     7561 tgaacagggg cgaagtatat cccgtgatga ggctgttaaa gatgcacaac agaaagccgc
     7621 tgaaatttct gccagggctg ctgatctgtc tgatagtgtt aaccagctgc gtgccgaagc
     7681 aacaaaatat gccatacgcc ttgacgcagc gcagcatacc gcaaatcttg ccgctgccgt
     7741 cagaggcaaa acaaccaaag ccgccgaagg aatgctcacc aacatgctcg gagatattgc
     7801 agcagaagct cagctttatg ctgaaattgc tgacaaacgc tacatcgcag gagtgacttg
     7861 tcaacggatt tatgaat
//
LOCUS       DAZFRN010000199         7845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFRN010000199 DAZFRN010000000
VERSION     DAZFRN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.201"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..97
                     /locus_tag="ACHKUB_004502"
     CDS             <1..97
                     /locus_tag="ACHKUB_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6657424.1"
                     /translation="GYRFNEQAAAKAGTAFSDGDVSWNVGVNWEF"
     gene            225..431
                     /locus_tag="ACHKUB_004503"
     CDS             225..431
                     /locus_tag="ACHKUB_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657425.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            494..970
                     /locus_tag="ACHKUB_004504"
     CDS             494..970
                     /locus_tag="ACHKUB_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO6657426.1"
                     /translation="MQIIIMTRDRYLEYGLMRMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVIFHEYGCTFLADQLGISMKTVCAHLYNAMEKSGLRGVSVKYLCSTADR"
     gene            1094..1609
                     /locus_tag="ACHKUB_004505"
     CDS             1094..1609
                     /locus_tag="ACHKUB_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO6657427.1"
                     /translation="MRFICDNSYFLEGVKEYINPGKFIISGMQTDNVVIISSSRISEI
                     VGFLYKNNISDANTLYFSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMS
                     VEKLTLSGRQVNVLMMLLYGSNGTEGARYLGMSEKTFSTHKQNLIKRLRVHNEVELLY
                     KGMLLVRKGRL"
     gene            1684..1872
                     /locus_tag="ACHKUB_004506"
     CDS             1684..1872
                     /locus_tag="ACHKUB_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO6657428.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            1968..2864
                     /gene="mscS"
                     /locus_tag="ACHKUB_004507"
     CDS             1968..2864
                     /gene="mscS"
                     /locus_tag="ACHKUB_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO6657429.1"
                     /translation="MEKLDVMDASGEAVSWIVRNQDILLGYAVNLVAAVVIIIIGGIA
                     ARMISGVLNNLMLGRNIDATVAQFLSVLIRHAIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDG
                     KIVVIPNGKIIAGEIVNFSREPERRNEFIISVSYDADIDRVKQVLTDIVMSEERVLKN
                     REITVRLNELGESSMNFVVRVWSRRDDLQSVYWDVLERIKRQFDSEGISFPYPQMDIH
                     LVRSGKQAESVTHNSRSVSSRQ"
     gene            2990..4210
                     /locus_tag="ACHKUB_004508"
     CDS             2990..4210
                     /locus_tag="ACHKUB_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFO6657430.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWSDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            4439..6184
                     /locus_tag="ACHKUB_004509"
     CDS             4439..6184
                     /locus_tag="ACHKUB_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO6657431.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLVQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            6285..7208
                     /locus_tag="ACHKUB_004510"
                     /pseudo
     CDS             6285..7208
                     /locus_tag="ACHKUB_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7338..>7845)
                     /locus_tag="ACHKUB_004511"
     CDS             complement(7338..>7845)
                     /locus_tag="ACHKUB_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO6657432.1"
                     /translation="TEPVDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQ
                     PVVTLAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIR
                     HNRARGKHQIDALSDIVRDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEEN
                     FSPAWTIK"
BASE COUNT         1966 a         1574 c         2099 g         2206 t
ORIGIN      
        1 gggatatcgc tttaacgagc aggctgcggc aaaggcaggt actgcgttca gtgacggtga
       61 tgtttcctgg aatgtgggtg tcaactggga gttctgaacc cggggctgag tcaccggggc
      121 gggcagcgtt ctgtatgcgg tcatgattcg ctgcctgctt ttctccccgg tggcatgact
      181 gacggttccg gggtattcct gaggagctga gagggagagt gtatatgctg aactatcgca
      241 taagttcagt gcaggtattt actgtaaacg gtcgggctga cagggtgcag gttcgtctgg
      301 aatgcgacga tgcagctgtt tttgaatgtt gtcttcttgc tgaaggagaa ggggaactga
      361 aagagctgag tctgtcagag ctggaagagc gggcactgat gtatgcggca gacagtttcc
      421 gttatgaatg atgagtcagt tataccggta atggtgaacg gagccggtat ccgggagata
      481 agggtctgag agtatgcaga ttattattat gacccgtgac agatatctgg aatatggcct
      541 gatgcgtatg ctgaacggat acaggctcac gacaggccgt gaactgtttg atgccggaaa
      601 gcgccgtctg ccacttcccg aagacagtta tgtgattctc tgtgaccgta atctggaaag
      661 gcttacatac tgtatgttct gtgggcgtcg ttttcttgtc attcctgttt cctctgtgag
      721 atgcctgaca gatatcaggc aggccatccg tcgtggtgcg tggctgttcg gacatacggc
      781 aagaccactg acccgtaccg agatggtggt ggtcttcggg gttattttcc atgagtacgg
      841 ctgtaccttt ctggcagacc agctggggat aagcatgaag acggtatgtg cgcatcttta
      901 caatgcgatg gagaaaagcg gtctgcgagg tgtcagtgtt aaatatctct gcagcaccgc
      961 agaccggtaa aaagccggtc tgatgaaggc tgttgcgacg ggaatttctg tgcatgctgt
     1021 gtcacgggca tcccggtgct ccggataatt aatgttatgt ggtcaggcgt gataaatttc
     1081 atgtggaacg ggtatgcgtt ttatttgtga taacagttat tttctggagg gagtgaagga
     1141 gtatattaat cccggtaaat tcattatcag cggcatgcag acagataatg tggtgataat
     1201 atcttcttcc cggatatctg aaattgtcgg ctttctgtat aagaataata taagtgatgc
     1261 gaacaccctt tatttttctt ctccggaagc gctccgtttt ctctggggag tggttgacag
     1321 tccggtttat gatatcagac atcttactga gcgatgttca gtaaacgata tcagtcatct
     1381 gtaccgcgca ttcatgtcgg tggagaagct gaccctgtca ggccggcagg taaatgtact
     1441 gatgatgctg ctttatggca gtaacggaac agagggggca cgatatcttg gcatgtcaga
     1501 gaaaacattc tccactcata agcagaatct gataaagaga ttacgggtac ataatgaagt
     1561 tgagttgttg tacaagggga tgcttctggt cagaaaaggg aggttgtgaa gcaggacagc
     1621 ttatttccgg taatgttaaa cgcagcctct gattcgttaa tttcataatc ataaggagtt
     1681 tttatgcgtc tgttgattgc ttttcttctt ccctggctga cttttttcac cattggccgg
     1741 cctctgacgg ggattttttg tctggtgtta cagataacgc tgctgggctg gataccggca
     1801 acaatatggg ctgtgtatgc gctttcgcag tataagacgg aacagaaaat ccgcgaagcc
     1861 acggggaaat gatgactgca gaagagcagg ttgtcatggc tgtgtatatg atgcgggata
     1921 aaggcccgga gattcctgaa aataaaatgg ttcgtggaat ataaaatatg gaaaaacttg
     1981 atgtgatgga cgcttctggc gaggcggttt catggattgt ccgtaatcag gatatattac
     2041 tcggttacgc cgtgaatctt gtggcagcag tggttattat tattatcggg ggtatcgctg
     2101 cacggatgat ttctggtgtg ctgaataatc tgatgctggg caggaatatt gatgccacag
     2161 tggcgcagtt cctttccgtg ctgatacgtc atgccattat agcctttacg ctgattgctg
     2221 ccctgggacg tgtgggggtt cagacagctt ccgtaattgc agtcctgggg gctgcaggtc
     2281 ttgctgttgg acttgccctg caggggtcat tgtcaaacct tgcagctggt attcttctcg
     2341 tgatgttccg tcctttcagg gctggtgaat atgctgacct gggtggtgtt gccgggactg
     2401 tgcagaatgt acatatattc tcaaccacga tgcgtacact ggatggtaaa attgttgtca
     2461 ttccgaacgg gaagattatt gccggagaga ttgttaattt ctccagggag cctgaacgac
     2521 gaaatgagtt tatcatcagt gtttcatatg atgcagatat tgaccgtgtg aaacaagtcc
     2581 tgacagatat tgtgatgtca gaagagaggg ttctgaagaa cagggaaatc actgttcgcc
     2641 tgaatgagct tggggagtca tccatgaatt ttgtagtacg ggtatggagc aggcgtgatg
     2701 accttcagtc tgtatactgg gatgtactgg agagaattaa gcggcagttt gattcagaag
     2761 ggataagttt tccatatcca caaatggata tccatcttgt tcgctccggg aagcaggcag
     2821 agtcggtaac ccataacagt cgctcagtca gttccagaca gtaaaatgaa aagcgcattt
     2881 tatttgtgaa agatgaacag cctgaatctg tcaggctgtt ttcattgtgt ttttatggtc
     2941 atttttggta tatgtactgt atatgatgtc atctgtataa ggatataaaa tgccaaagaa
     3001 aataatgaca gggattgatg tttttgacgc tgccatggag cgtattgaat gggtgtttga
     3061 tacgttttca tcagtttgtc tttctttttc cggcggaaaa gactccactg tcctgtttca
     3121 cctgatggcg aatattgccc gccgaaaaaa acgccgtttt tctgtactgt ttgttgactg
     3181 ggaggctcag tatcagtgta ccattgagca tgtcaccagg ttgagggaaa tgtacgatga
     3241 tatcacagat acttttttct gggtggcgct gcctctgacg accgttaatg gagtgtctca
     3301 ggtacagcct gagtgggtat gttgggagga cggggctgaa tgggttcgcc agccaccaga
     3361 agatgccata accagagcgt ctttttttcc tttttatcag aatgcgatga catttgaaga
     3421 gtttattccg gcatttaatc attggtttgc ccgggaaaaa gggatggttg ctgcgattct
     3481 tactggtgtt cgcgctgatg agtcactgaa tcgttttatg ggactggtat ccggacgtaa
     3541 gctccgttat gcggaggata aaccctggac cacggcatcg gctgagggat tttactacac
     3601 catgtatcct ttgtacgact ggaaaacaag agatgtatgg atttaccatg cgagaacacg
     3661 tgctgcctat aactctcttt atgacctgat gtacagggct ggagttcctc ttcgcaatat
     3721 gcgggtgtgt gagccttttg gaccggagca acgtaagggg ttgtggcttt atcatgtact
     3781 ggagccggat acatggggca gaatgtgtgc tcgcgtatca ggtgctgtag ggggagcgct
     3841 ctatgcaaat gaaagtggtg cttattttgc tttgaggaaa cagataacaa aaccagttca
     3901 ccatacatgg cagagttatg caatgtttct gctggagtca atgcctgaga aaacggcgga
     3961 gcattacaag aataagattg ctgtttatct tcggtggtgg cagtctcatg gatggtcgga
     4021 tggcatccct gatgagcagg ataatgacca gggatgtaaa gatattccct cctggcgtcg
     4081 catctgtaag acgttactga aaaatgattt ctggtgtcgg atgctggcgt ttagtccgaa
     4141 taagccccga cattatgagc gctacctggc cagaatgcgt gaaaaaagga aagagtgggg
     4201 aatattgtaa attatattta aacatccgaa atatgacggt taatccagga tagtagctaa
     4261 aaaataagat ttcttgctga tttatccctg tttgatatgt aaaatctgag gggcttataa
     4321 gcaacggata tactagtgct acaatatatg ataagtttta tatattaaat gttcatcatc
     4381 tgtcaggggc tgttttatgg ctaaactgat aaatatatca ggtgttaccg aggagcggat
     4441 gactgccggg gaaaagcggg ttgcatcccg tcttgaggca tttatgagta atgagtgtct
     4501 ggtctggtac gatattccgg tgggacggaa aaaccgacat cctgattttg tcattattga
     4561 cccggagaat ggtctggtat ttcttgaagt caaggactgg accgttcaca cgttgcatca
     4621 ggtaaatcac gaacaggtca ttcttgaaac taacggtatt cttaaatcag aaatcaatcc
     4681 gctggtacag gttcgtcgtt atgcctgtga taccgtaaat gctttacctg cagactcacg
     4741 tttgcgacag aatgatggtc agtataaggg gcgtctgaat ctggcctggg cgtatggtgt
     4801 tgtgtttacc cgtattaccc gccagcaact gaaggctctg gctgggaatg atgaaaatgc
     4861 tgtggaaagg attttccctt cagcgcagac aatctgtcag gatgatatga ctcagtctgt
     4921 tttacctgat atattccgcc aaaaaattgc cgggatgttt acgatgggat tcagaacccg
     4981 ggtgacacca caggttagag atattctgcg tgctcatctg tttcctgaaa tgaccgtcag
     5041 acagcactca caaatcaaaa taatggatat tcagcaggag attctggcca gaaatattgg
     5101 tgatgggcat cgtgttattc atggggtagc cggctctgga aaaacaatga ttcttttgtt
     5161 ccgttgtctt tacttggctg aaactacccc tggtaagatt ctggtgctat gttacaacat
     5221 tactctggcc agctacctgc gggaatgtat tgagtccagg ggactgaagt cacgggttac
     5281 ggtattccat tttcatagtt ggtgcgcctc aatgataaag cgccatggaa ttcaggtgac
     5341 tgctggagga aaggattacc cggagaaatg cttcagtgcg ctggaagatg ccgtgaacag
     5401 cggaacaatt actgatactg gatacgatgc cgtacttgtg gatgaggggc atgattttga
     5461 gagtcgctgg ctggctctga tagcccgcct gtttgataac gcatcccgtt cactgctcct
     5521 gatgtacgat gacgcacagt ccatttacag acgggaacgg gctctgaact tttcgcttgc
     5581 cagtgtgggg attcaggcgc agggacgtac ttccgtcctg ccggtaaatt accgtaatac
     5641 gaaacgcatt ctgcactttg cttatgcatt ctcccgtgag tattttgaga agcatcagaa
     5701 ccgtgaaata ccgtttgttc agccacaggc gggcggtgaa gagggcacag agccggaaat
     5761 tctgcgttgt gcttctgaga gtgatgaggc agtgcaggtt gtgggctggc tggagaaacg
     5821 ctatacactg tgcggtcact ggagtgatat ggctgttttg tgtcctgcag agttttcagt
     5881 gaaacatctg aaagaggtca tgacacaacg cgggatacct tatgccacct gctttgacag
     5941 tgaaggtaag aagaaatatt cgcgacgcaa ggatgttgtg catttactga cctaccagag
     6001 cagtaaggga ctggagtttc cgtatgttgc ggttatcaat gcttctttcg ttcactcagg
     6061 tgctgcggat gagtctgaag ttattccggc gctttatgtt gcgttcaccc gggctacacg
     6121 ggaattactg gtgacctgtt atcgtgaaaa cagtatcagt cgtcatcttg aggattttgt
     6181 ataagttatg ttttattatt gctgatatgt aatcattcgt tttttgataa tctaagatgg
     6241 gtatttagtg tagtggcaca ctaaatttgg ccacctgatg aaaggtgata ttctcaccac
     6301 aacacaaaac aggtgactta atgaacaaga aaaccaaacg aaccttcacc cctgagttca
     6361 ggctggaatg tgcacagctg attgttgata agggctactc atatcgacag gccagtgaag
     6421 cgatgaatgt cggttcaacc acgcttgaga gctgggtacg ccagctcagg cgagagcgcc
     6481 agggtattac gccctctgcc acacccatta ctccagacca gcaacgtatc cgcgagctgg
     6541 aaaagcaagt tcgccgtctg gaggaacaaa atacgatatt aaaaaaggct accgcgctct
     6601 taatgtccga ctcgctgaac ggttcacgat agccgccaga ctaagtgaca gccacacggt
     6661 tgtcagcctg tgttctgctc tggaaataca ccgcagcagt taccggtact ggcgaaaacg
     6721 acgcgatacg gttaatccgg cacgagtcag gttgtgcagc gaaatacgcc gggcgtggaa
     6781 ccaaagtcgg ggctctgcgg gcgcgcgcac tctggctgaa atgctgactc aaaacggcgt
     6841 cccgatgagc cgttaccgtg ccggacgtct gatgaaatat ctgaacctga gcagttgtca
     6901 gcccggaaaa catcagtata aaaatgcccg tcaggagcat accagcctgc cgaatctgct
     6961 tgagcgtcag tcggcgggga aactgctggg ataacagccc catggaacgc ttcttccgca
     7021 gtctgaaaac agaatgggtg ccaacgaatg gttacgcagg caaggacgag gcccggcagc
     7081 aaattaatga ttacatattg aactactaca acagcgtcag acctcaccat tataacggtg
     7141 ggctgacgcc ggaagagtca gagaacagat accattttta ctgtaaaacc gtggccaata
     7201 ttacttgacc actacattag cgagtattca gagttgaatt aatatccccc atgtttttga
     7261 tctgtcaata gctgtccatt acatggggtg atcatatatc agtcggtaat tactggataa
     7321 agtcaccccc taaatgatta ctttattgtc catgccggac taaagttttc ctcctcaaat
     7381 agttctgtta acccacttat ttgttttaat ctcagtacct catctgtatc catgcccagt
     7441 tcactaccta ttcggagatc atcccatccc attcgggaaa gatctcggac gatatcggag
     7501 agcgcgtcta tctggtgctt tcctctggcc ctgttatgcc tgatggtcga ggcgatagag
     7561 taagctttaa agttattatc atgcttaatg catgtgacag gtaaatatcc ttttagtttt
     7621 ttctctagtt ttgttgtgct tttcccaagc atttgtctgt gaaatccgtc aataacggtg
     7681 tatttcccct tttgagctaa ggtgacaaca ggttgagtga atccatcaac caacagggag
     7741 tgtgttaata accttttttc ggagggagcc atgacgttag gattatagtc gtttgcttca
     7801 attagattgt tttttatcca tataacacaa tcaacaggtt ctgtg
//
LOCUS       DAZFRN010000200         7810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFRN010000200 DAZFRN010000000
VERSION     DAZFRN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.202"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..936
                     /locus_tag="ACHKUB_004512"
     CDS             <1..936
                     /locus_tag="ACHKUB_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO6657433.1"
                     /translation="YLITSVRQTLEEQGAALQNLSKQLDSGQYQCVLNLIMNCKGHVI
                     LSGMGKSGHVGRKMSATLASTGIPSFFIHPAEAFHGDLGMITPCDLLILISASGETDE
                     ILKLVPSLKNFGNRIIAITNNGHSTLAKNADAVLELHMANETCPNNLAPTTSTTLTMA
                     IGDALAIAMIHKRKFMPNDFARYHPGGSLGRRLLTRVADVMQHDVPAVQLDASFKTVI
                     QRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSATAAQMMTREPLTLPED
                     TMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1008..2156
                     /locus_tag="ACHKUB_004513"
     CDS             1008..2156
                     /locus_tag="ACHKUB_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFO6657434.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLSDIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2180..3856
                     /locus_tag="ACHKUB_004514"
     CDS             2180..3856
                     /locus_tag="ACHKUB_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFO6657435.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDISADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            3866..4606
                     /gene="kdsB"
                     /locus_tag="ACHKUB_004515"
     CDS             3866..4606
                     /gene="kdsB"
                     /locus_tag="ACHKUB_004515"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO6657436.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4603..6630
                     /locus_tag="ACHKUB_004516"
     CDS             4603..6630
                     /locus_tag="ACHKUB_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFO6657437.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLSVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISKAREAMHTIVTGDLSKYNLAPAFVADESECSDIVLVVDQTFND
                     MSVTYGNAGQNEFSAMLEAAMAENPQATIWVKVHPDVLKGKKTGYFADLRATQRVRLI
                     TENISPQSLLRHISRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSVTLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLLRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWQVEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPSLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKGKKVILVPGQVEDDSSIKTGTISIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTFDEHHCGRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTSRKPMFITRKKAGWVIRYYRKLIMFCKVRFG"
     gene            6665..>7810
                     /locus_tag="ACHKUB_004517"
     CDS             6665..>7810
                     /locus_tag="ACHKUB_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFO6657438.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRSYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPGLLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYILMKT"
BASE COUNT         1994 a         1870 c         2037 g         1909 t
ORIGIN      
        1 tatttaatta cttctgttcg tcagaccttg gaagaacaag gtgcagcatt acaaaacttg
       61 tctaaacaac tggattccgg gcagtaccag tgtgtcctta atttgataat gaactgtaaa
      121 gggcacgtta ttctttcggg aatgggtaaa tcagggcatg tcggtcgtaa aatgtcagcg
      181 acgctggcct ctaccggtat cccgagtttc tttattcatc ctgcagaagc gttccatggc
      241 gatctgggta tgattacgcc ctgcgatctt ctgatcctta tttctgccag cggtgaaacg
      301 gatgaaatcc tcaagctagt tccttcactg aaaaatttcg gcaaccgaat tatcgccatt
      361 accaataatg gacattccac gctggcgaaa aatgctgatg ccgtgctgga gctccatatg
      421 gcgaatgaaa cctgcccgaa taatcttgca ccaacaacgt ctaccaccct gaccatggcg
      481 atcggcgatg cattggctat cgccatgatc cacaaacgca aatttatgcc gaatgatttt
      541 gcgcgctatc acccgggcgg ttcattaggt cgtcgcctgc tgacccgcgt tgctgatgtc
      601 atgcagcatg atgttcctgc ggtacagctg gatgcgtcat ttaaaaccgt gattcaacgt
      661 atcaccagcg gatgccaggg aatggtgatg gtagaagacg cagaaggtgg gctggcgggc
      721 attataaccg acggtgacct gcgtcgcttt atggaaaaag aggattctct gacatccgct
      781 acggctgcgc agatgatgac acgtgaaccg ctgacgctac cggaagacac catgatcatt
      841 gaagcggaag aaaaaatgca aaagcaccgc gtctcaacgt tattggtgac caacaaggca
      901 aataaagtca ctggccttgt gcgcattttc gactaattaa gcaacggggt ttgagaggtt
      961 agtctctcat tgagtttgcg ccccactgga actttgataa tcgttacatg ttgataaaag
     1021 tgaagtctgc cgtatcctgg atgcgtgctc atctgtctgc catctcactg tcagatatcc
     1081 aaaaacacct ggtgaaaatc atcattctgg caccgatggc ggtgctgctg atctatctgg
     1141 ctatcttcag ccagcctcgc tatatgagcg agtcgaaagt cgccattaaa cgctcggatg
     1201 atttaaacag cggcagcctg aattttggtc tgcttctggg tgcctctaac cccagttccg
     1261 cagaagatgc gttgtatctg aaagagtaca tcaactcgcc ggatatgctg gcggcgctgg
     1321 ataaacaact aaattttcgt gaagcgttca gccacagcgg gctcgatttt cttaatcatc
     1381 ttagcaagga tgaaaccgca gaaggcttcc tgaagtacta caaggaccgt atcaacgtct
     1441 cgtatgacga taaaaccgga ttactgaata ttcagacgca gggctttagc ccggagtttg
     1501 cgcttaagtt taaccagacc gtgctgaaag agtcagagcg ctttatcaat gagatgtcac
     1561 atcgcatcgc gcgtgaccag cttgcctttg cagaaacgga gatggaaaag gcacgccagc
     1621 gtctggacgc cagcaaagcg gaattgctct cttatcagga caacaacaac gttctggatc
     1681 cacaggcaca ggcacaggcg gcgagcacgt tagtcaatac gctgatgggc cagaagatcc
     1741 agatggaagc ggatctgcgt aacttgctga cgtatctgcg tgaggacgcc ccgcaagttg
     1801 tgagtgcgcg taatgcgatt cagtcattgc aggcacaaat tgacgaagaa aaaagcaaaa
     1861 tcactgcgcc acagggtgac aagctaaacc gtatggcggt ggattttgaa gaaatcaaat
     1921 caaaagtaga gttcaacacc gagctgtaca aactgaccct gacctccatt gaaaagaccc
     1981 gtgtagaagc ggctcgtaag ctcaaggtgc tgtcagtgat cagttcgcca cagttgccgc
     2041 aggaatcgtc ttttccaaat atcccttatt tgatcgcctg ctggttactg gtgtgctgcc
     2101 tgctgttcgg caccctgaaa ctgttgctgg ctgttattga agatcaccga gactaacgct
     2161 gtcgctgaat gagtttgtga tgaaattatt taaatcaatt ttactgattg ccgcctgtca
     2221 cgcggcgcag gccagcgcgg ccattgatat tagcgctgac ccaaacctga caggagccgc
     2281 gccgcttacc ggtattctga acggacagaa gtcggatacg caaaacatga gcggcttcga
     2341 caatacccca ccgcccgcac cgccggtggt aatgagccgt atgtttggtg ctcaactttt
     2401 caacggcacc agcgcggata gcggtgcgac ggtaggattc aaccctgact atattctgaa
     2461 tccgggtgat agcattcagg ttcgcttgtg gggtgcgttc acctttgatg gtgcgttaca
     2521 ggttgatccc aaaggtaata ttttcctgcc gaacgttggt ccggtgaaag ttgctggcgt
     2581 cagtaatagt cagctaaatg ccctggtcac atccaaagtg aaggaagtat accagtccaa
     2641 cgtcaacgtc tacgcctcct tattacaggc gcagccagta aaagtgtacg tgaccggatt
     2701 tgtgcgtaat cctggtctgt atggcggtgt gacgtctgat tcgttactca attatctgat
     2761 caaggctggc ggcgttgatc cagagcgcgg aagttacgtt gatattgtgg tcaagcgcgg
     2821 taaccgcgtg cgctccaacg tcaacctgta cgacttcctg ctgaacggca aactggggct
     2881 ttcgcagttc gccgatggtg acaccatcat cgtcgggccg cgtcagcata ctttcagcgt
     2941 tcagggcgat gtctttaaca gctacgactt tgagttccgc gaaagcagca ttcccgtaac
     3001 ggaagcttta agctgggcgc gccctaagcc tggcgcgact cacattacga ttatgcgtaa
     3061 acaggggctg caaaaacgca gcgaatacta tccgatcagt tctgcgccag gccgtatgtt
     3121 gcaaaatggc gataccttaa tcgtgagcac tgaccgctat gccggtacca ttcaggttcg
     3181 ggttgaaggc gcgcactccg gtgaacatgc catggtactg ccttatggtt ccactatgcg
     3241 tgcggttctg gaaaaagtcc gcccgaacag catgtcgcag atgaacgcgg ttcagcttta
     3301 tcgcccatca gtggctcagc gtcagaaaga gatgctgaat ctctcgctgc aaaaactgga
     3361 ggaagcatca ctttctgccc agtcctccac caaagaagaa gccagcctgc gaatgcagga
     3421 agcacaactg atcagccgct ttgtggcgaa agcacgcacc gtggttccga aaggtgaagt
     3481 gatcctcaac gaatccaata ttgattctgt tctgcttgaa gatggcgacg tcatcaatat
     3541 tccggagaaa acatcgctgg ttatggttca tggcgaagtg ctgttcccga acgcggtgag
     3601 ctggcagaag ggtatgacca ccgaggatta catcgagaaa tgtggtggcc tgacgcaaaa
     3661 atcgggtaac gccagaatta tcgtcattcg tcagaacggt gctgccgtca acgcggaaga
     3721 cgtggattca ctcaagccgg gcgatgagat tatggttctg ccgaaatatg aatcgaaaaa
     3781 tattgaagtt acccgtggta tttccacaat cctctatcag ctggcggtgg gtgcaaaagt
     3841 gattctgtct ttgtaaggag ttgagatgag caaagcagtt attgtcatcc cggctcgcta
     3901 tggctcctcg cgcctgccgg gtaagccact gctcgatatt gttggtaaac cgatgatcca
     3961 gcatgtttac gagcgtgcgt tacaggtggc gggcgtcgca gaagtttggg tggcaacaga
     4021 cgatcagcgt gttgaaaagg ccgtgcaggc atttggcggg aaagccatca tgacgcgcaa
     4081 cgatcatgaa tccggcaccg atcggctggt cgaggtgatg cataaagtcg aggcagatat
     4141 ctacattaac ctgcagggcg acgaaccaat gattcgcccg cgggatgtag aaacgctgtt
     4201 acaaggaatg cgtgatgacc ccgcgttgcc agtggcaacg ctatgccacg cgatttctgc
     4261 cgaagaagcg gctgagccaa gcacggtaaa agtggtggta aatacccacc aggatgcgct
     4321 ttatttcagc cgttcgccga ttccgtatcc gcgtaatgct gaaaaagcgc gctacctgaa
     4381 acacgttggt atttacgctt atcgtcgcga tgtgctgcaa aactacagcc agttaccgga
     4441 gtccatgcca gagcaggcag aatcactgga gcagctgcgg ttaatgagcg cggggatcaa
     4501 catccgcaca tttgaggttg ccgcaaccgg tccgggcgtc gacaccccag catgcctgga
     4561 aaaagtgcgc gccctgatgg cacaggaact ggctgaaaac gcatgattgg cattttctcg
     4621 tccggtatct ggcgtattcc gcatctggag aaatttctgg cgcaaccgtg ccagaaactt
     4681 tctctgctgc gccctgttcc gcaagaagtt gatgctatcg ccgtgtgggg acatcgtccc
     4741 agcgcggcaa aaccggtcgc catcgccaaa gcagcgggaa aacccgtcat ccgtctggaa
     4801 gatggatttg tgcgttcgct ggatcttagc gtcaatggtg cgccgccgct ttctctcgtg
     4861 gtggatgatt gtggcattta ctacgatgcc agcaagcctt cagcactgga gaaactggta
     4921 caggataaag ccggaaatac agctctgata agtaaggcca gagaggcgat gcacaccatc
     4981 gtgaccgggg atttgtcgaa atataacctg gcgcctgcgt ttgtggctga tgagtcagaa
     5041 tgttcagaca tcgttctggt tgtcgatcag acatttaatg atatgtcagt gacgtatggt
     5101 aatgctggtc agaatgagtt ttctgccatg ctggaggccg cgatggcgga aaatcctcaa
     5161 gcgacaattt gggtgaaggt gcacccagat gtactgaaag gaaagaaaac aggttatttc
     5221 gccgatctgc gcgccacgca acgagtacgt ttgattacgg agaatatcag tccgcagtcg
     5281 ctgttgcgac atatttccag agtttacgtc gtgacctccc aatacggctt tgaggcctta
     5341 ctggcaggaa aaccagtgac atgcttcggt caaccctggt atgcaggctg gggcttaacg
     5401 gacgatcgtc atccgcagtc ggctttgtta tctgcccgac gcggttctgt cacgctggaa
     5461 gaactttttg ccgcagcata cctgcgttac tgtcgctata tcgacccact aacgggggcc
     5521 gcaagtgacc tgtttaccgt actgcaatgg ctgcaattac tgcgcaacca tcagcaacag
     5581 cgtaatggct atttatgggc gccaggctta acgctgtgga agtcagcaat cttgaaacct
     5641 ttcttgcgta ccgcgacaaa caggctgagt tattcacgtc gctgtactgc tgccaccgcc
     5701 tgcgtggtat ggggtgtaaa aggagaacag caatggcagg tcgaagcgca gcgaaaatca
     5761 ctgccattat ggcgaatgga agatggtttt ctgcgttcat ccggacttgg ctcggacctg
     5821 ctgccatcgc tatcgctggt gctggataaa cgcggaattt actatgacgc cacgcgcccc
     5881 agcgacctgg aagtgctgct taatcacagc cagttaacgc tggcgcagca gatgcgagct
     5941 gagaagttgc ggcaacgact ggttgaaagc aaactgagta agtacaacct gggagccgat
     6001 ttctctctgc ctgccgaagc caaaggtaaa aaagttatcc tggtgccggg tcaggtagag
     6061 gacgattcct ctattaaaac aggcactatc tcaattaaga gcaaccttga gttattacgc
     6121 acagtacgcg agcgtaatcc gcacgcctac attatttata aaccgcaccc ggatgtactg
     6181 gtggggaatc gcaagggcga tattccggca gaactgattg ctgaacttgc tgattatcag
     6241 gcactggacg cagatattat tcaatgcatt cagcacgcag atgaagtgca taccatgacg
     6301 tcgctgtcgg ggtttgaagc gttattacat ggtaagcacg tacattgtta cggcctgccc
     6361 ttctatgctg gttggggttt aaccttcgat gaacatcact gcgggcgtcg tgagcgagaa
     6421 ttaacgttag cggatttgct atatcaggcg ctgattgttt atccaactta tatccaccca
     6481 acacggctac aacctattac tgttgaagag gcagcggaat atttgatcca gacgtcgcgc
     6541 aagccgatgt ttattacccg aaaaaaagcg ggatgggtaa tacgctatta ccgcaaatta
     6601 attatgttct gcaaggttag atttggctaa acaatttcat agttgactat tacgcaaatt
     6661 atttatgcaa ggtaatgcac taaccgtttt attatccggt aaaaaatatc tgctattgca
     6721 gggaccgatg ggaccctttt tcagtgatgt agccgagtgg ctggagtcat taggccgtaa
     6781 cgctgtgaat gttgtattca atggagggga ttgtttttac tgtcgccacc gacattatca
     6841 ggcttactac cagacaccga aagagtttcc cggttggtta cgggatctcc accggcaata
     6901 tgactttgat accatcctct gctttggtga ctgccgccca ttacacaagg aagcaaaata
     6961 ttgggcaaag tcgaaaggta tccgctttct ggcatttgag gaagggtatt tacgcccgca
     7021 gtttattacc gttgaagaag gcggagttaa cgcatattca tcgctaccgc gcgatccgga
     7081 tttttatcgt aagttaccag atatgcctgc gccgcacgtt gagaacttaa aaccttcaac
     7141 gatgaaacgt ataggtcatg cgatgtggta ttacctgatg ggctggcatt accgccatga
     7201 gttccctcgc taccgccacc ataaatcgtt ttccccctgg tatgaagcac gttgctgggt
     7261 tcgttcatac tggcgcaagc aactttacaa ggtaacacag cgtaaggtat tgccgaggtt
     7321 aatgaacgag ttggaccaac tttattatct tgccgttttg caggtatata acgatagcca
     7381 gattcgtaac cacagtaatt ataacgatgt gcgtgactat attaatgaag tcatgtactc
     7441 attttcacgt aaagcgccga aagaaagtta tttggtgatc aaacatcatc cgatggatcg
     7501 tggtcacaga ctctatcgac cattaattaa gcggttgagt aaggaatatg gcttaggtgg
     7561 gcgagtcatt tatgtgcacg atctcccgat gccgggacta ttacgccatg caaaagcggt
     7621 ggtgacaatt aacagtacgg tgggaatctc tgcactgatt cataacaaac cacttaaagt
     7681 gatgggcaat gccctctacg acatcaaggg cttgacatat caagggcatt tacaccagtt
     7741 ctggcaggct gattttaaac cagatatgaa gctgtttaag aagtttcgtg gatatatatt
     7801 gatgaagaca
//
LOCUS       DAZFRN010000201         7780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFRN010000201 DAZFRN010000000
VERSION     DAZFRN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.203"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..261
                     /locus_tag="ACHKUB_004518"
     CDS             <1..261
                     /locus_tag="ACHKUB_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657439.1"
                     /translation="FINVDFSGFLFIISRLFSFLCFVLFTLFSIICIISIGKNEGLLN
                     YLVYMVMIIVLETVGFWMYDTFPSKKKICELNKELRKIKIPE"
     gene            complement(314..862)
                     /locus_tag="ACHKUB_004519"
     CDS             complement(314..862)
                     /locus_tag="ACHKUB_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFO6657440.1"
                     /translation="MKLKLNNVRLAFPSLFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWEPIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(878..2179)
                     /locus_tag="ACHKUB_004520"
     CDS             complement(878..2179)
                     /locus_tag="ACHKUB_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFO6657441.1"
                     /translation="MPDVHARLSPSSAHRWLRCAGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERAQEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDFKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLTADLI"
     gene            complement(2172..2690)
                     /locus_tag="ACHKUB_004521"
     CDS             complement(2172..2690)
                     /locus_tag="ACHKUB_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFO6657442.1"
                     /translation="MPEAWKDISGFEGIYQISDAGNLRSLDRTVLNKGSGCTYPIKGK
                     SLKPRYDADGYLITDLWKAGKKVTVKIHRLVAAAFIPGSAPEVDHINGRRDDNRAVNL
                     RWASLSQNRANSHTRKNKSGVVGVRFSEGKKNPWQAYGRLNGKFKSLGHFPTKQLAAA
                     ARRKHIQEIMNA"
     gene            complement(2693..3595)
                     /locus_tag="ACHKUB_004522"
     CDS             complement(2693..3595)
                     /locus_tag="ACHKUB_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657443.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNNAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            complement(3592..3741)
                     /locus_tag="ACHKUB_004523"
     CDS             complement(3592..3741)
                     /locus_tag="ACHKUB_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657444.1"
                     /translation="MKPEHIHRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            complement(4467..5096)
                     /locus_tag="ACHKUB_004524"
     CDS             complement(4467..5096)
                     /locus_tag="ACHKUB_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO6657445.1"
                     /translation="MNLANRAKKRRTELNLTQVEVAKRAGISQQSIEAIENGKTLKPR
                     NLLALASALECDPKWLLLGGDVVTDFNYGARRVPVLSYVQAGAFTSAELLREEGDFEY
                     ILTTAELSENSFALRIRGDSMEPEFKEGDIVIIDTDVYPTPGEFVAACNGSHEATFKK
                     YRPVGIGAKGEEDFELVPLNSDYPICRSWEKPIKIIGTMVEHRIFRRKR"
     gene            5204..5428
                     /locus_tag="ACHKUB_004525"
     CDS             5204..5428
                     /locus_tag="ACHKUB_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6657446.1"
                     /translation="MTSSTLASRIKERRKALGITQTSLAESVGMRQQSVQYLESGRAT
                     RTGFILELAKVLKCDPDWLLNGENSEQQKV"
     gene            5432..7693
                     /locus_tag="ACHKUB_004526"
     CDS             5432..7693
                     /locus_tag="ACHKUB_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFO6657447.1"
                     /translation="MPDNKPWGATPDEWFHFDLVLGMTDRLLPVVSNPCAELSPNSKL
                     SHNFGKTPSRYNGQRKVVGIPDWANYVVTENDFARWSNEPDYGICVRTGDGVVALDCD
                     INDAGMQEIVRNIILSCLGELPPRRWRADSHKCLYLIAVDGDYRKRGHRLEGENKQIE
                     LLAKGQQFVACGTHPAGERIQWDCGLPGEPLKITSEQLESLWQRLADNLPVKDSYTAG
                     AGRQRDLSCVDPSATDDVADWLDANGWTLSVSSDGSRNLKPFRYESEYSNGCSETSIK
                     YFPKGTGGFEQGHFKSMHNTDAGLTDADWLEGYGYTQSLFEDLTVAEDGDKPGFTDIN
                     TDMTAHFLERFIYVIEGDQVCDLSRPPYQCMMDMKSFKNLMAPYQFPPEGKGQPIPAT
                     KRWIEHRHKKIAETTGYKPGAGRIIERFDGRFEINEFYMPEHPRTADTSKVSTFLNHM
                     AYLVPDAWQREFFIARLGWMVQRPERRCPISILHVATAHGTGRGWVSQLMERALGPWN
                     CARTRMKILCDNQFHDYLYNTLLCTIDEVRENDKRYEVNDKIRDVLTEPRFEVNRKYG
                     SKKTMDIYTGFLFYTNHFDALALPEEDRRIAVLGGPDFAATEEHYASLYGALSDSDFI
                     AQVYWYLMGVDLSRFNWQRAPETKERLLMIESNKSDVEAALIEILDNPPVPAMTYQQI
                     VNAILAEAGMDVEINQKHITKLLREKTKREAVKLKVDGMTVRMWLLVKNTDFSNEELR
                     EIYKNCEILQSGL"
BASE COUNT         1880 a         1794 c         1924 g         2182 t
ORIGIN      
        1 tttattaatg ttgatttttc aggtttttta tttattatta gtcgtctgtt ttcctttctg
       61 tgttttgtgc tgtttaccct tttttctatt atctgcatta tttccatagg gaaaaatgaa
      121 ggccttttaa attatcttgt gtatatggtt atgataatag ttttagagac tgttggtttt
      181 tggatgtatg atactttccc gtctaagaaa aagatctgtg aactaaataa agaattgaga
      241 aagattaaga ttcctgagtg attatacgcc taatcagtag cccggtgttt aaccgggcgt
      301 ctatgaagta gggttaaatt agtgcttcag catcagcacc ttcgctgata tcgtcgaaat
      361 cgtcagcgct tgccactccg ccgccagcga atgcatcgcc gtctcgcagg aactggactc
      421 cgccgagtga ggcattaatg cgtttaccga aattattgtc ctgtgcccag atatcgataa
      481 cggcgtttac atagcaccct gcatagggac gtccgtcagc ctgaataagt ggcgaacgat
      541 cgcgatcaag aacagctggg cgcgctttgt tagcagcatt caggaagaaa ttaccgggga
      601 agccttcata ctctgctttt tcatcaccat catgcaggca cagattgagt tttttctcca
      661 gctggttata aatgggctcc cacttctctc cccatttttc cttcgctacc tgcttcatag
      721 ctttacggat ttcttccagt tgtgggtgtt tgggtgacat taaaaatact gcggagaaac
      781 gtggatcgcc ttcgccgttt acagttttag cttcaaacag agacgggaag gccagacgaa
      841 cattgttcag cttcagtttc atgggtattt ccttaaatca gatgaggtct gcggttagcg
      901 tatcgtcgga tacgtcgtcg aaatcattta cagggttgat attgagtgcg gggcgtgggt
      961 ctgactcggg aacgacggtt ggtttaccat cagctcgtgt tatcagtgcc tcgacttttg
     1021 accaacggcg cggactggcc tttttgataa gtttttcggc ttttgtggga ctaataagtt
     1081 taaagtcgaa tacttcttca gttttgtacc tgaactggtc cttcagaagt gcgcgagctg
     1141 cctcttcatc actccaggcc cggttacctt gttttcctgt taccagttta aaccccggta
     1201 ccggatgtcc ggcattgagt tcattgtgaa cccggtcccg tactgccttt agccaggatt
     1261 caataaagtc ggcctgacta tagatctccg caagctgctc aatggttaac agaggtacac
     1321 gtgcgctggc attggtgatt atttcgctga caggctttgt cagatcttca aaatcgctgg
     1381 ccgctgtttg taaatgctgc attttctggg ctgtgcaaat agcttttgct ttacagaagc
     1441 ggcactgttt ttctccaggt atgaagtttt ctagcggtag tgtctcaatg ccttcgcatt
     1501 cagcaatatt gagaacaagg atcgcactgg ttgcggcctc ctgtgctcgt tcaccgaaag
     1561 actgaagctc ctgtacggtt aaagaccatt ctgaaacgtg gttgagcctt ggctggtgaa
     1621 taaataatct tacagtctca aagtcataca gcatgctgaa ttgttcgaga gcacccagag
     1681 catacagttg tagttgctca ttttgttctg catcaatgcg gacgcctttg ccatatttca
     1741 ggtcgtggat ttgtaattcg ttaccagcga tgattatgcc gtcggcagtt ccgaaagatt
     1801 cttccacccc cgttatatgt gagaaatcaa cacgttgttc aaccaatagt tcattattct
     1861 gtgcaagagt ccagaccgta tcaacatacc ggccaacggc ttcgaccatt tcatcatcca
     1921 cctgtgggcc agatgtatca tcaggatttt cgcgaagggg gtatgagccg agaaacatag
     1981 aaacattgca tccggcgtag tgttccgggt ggctttgcct gtttcgtaga actttttcag
     2041 caagcgcgtg cgctgcagtg ccctcgattg caaaagttgt ttctttatcc ggttgtgtgg
     2101 cctccagcgc cagactaccc gcacacctta gccaacgatg tgcagatgat ggtgataatc
     2161 gtgcatgaac atcaggcatt catgatctcc tgaatgtgtt ttcgccgcgc agctgctgcg
     2221 agttgtttgg tggggaaatg accgagagat ttgaatttgc cgtttaatcg tccgtaggcc
     2281 tgccacgggt ttttcttacc ttcgctgaac cgaacgccaa ccactccaga tttatttttt
     2341 ctggtgtgag agttagccct gttttgtgaa agagaggccc agcgaagatt taccgcccgg
     2401 ttgtcatctc gacgaccgtt aatgtggtca acttcagggg cagatccggg aataaatgca
     2461 gcggcaacga gacggtgtat tttgacggtt acctttttac ccgctttcca taagtcggtg
     2521 atgagatatc cgtctgcgtc atagcgtggt tttagtgact tacctttaat gggataggtg
     2581 cagccagaac ctttgtttaa tacagttctg tcgaggcttc tcaggttgcc agcgtctgaa
     2641 atctggtaaa ttccttcaaa gcctgaaata tccttccagg cttccggcat gattaaccct
     2701 ccagtgcttt ttcagccagg gtgattactt cagcgagatt ttcatctgtt acttcaccaa
     2761 gtttcctggc tccctgtttt tccagaattg caatagcttc tgcccggtaa cccccttttg
     2821 ctaactggag gatcaaccct tcagcttgtt tgcgtagtgc cgcgaaatca attgtatggt
     2881 catctttggc gttattattc tggctggaat ttgctgcgtc tctgcgtgca aattcttcct
     2941 gcagctgaag gtactcaaca cggttgatct cgatatggcc ttttttcagc atctcgttca
     3001 acttgcgtaa ggtgtggagt tcactggctg ctgtgccgga tacatttttg acgtaaaacg
     3061 gccccgtgcg ttctccatct ttgttactgg cctttttcgg cttaacttca tcacgcccat
     3121 ctgcaggtgc atcaagtagc tgctcggcaa aagcacgtcg ctcgccgatg gttggcaggt
     3181 cgtcccagaa cttaagaatg ttacgggaca ggtccaggag agcaggttta agcagcgccc
     3241 tggctcgttt gacgccctgt aatgcgctgt cgagagcatc aatctgaact actcgtttat
     3301 cgccttcagc atcacggtag gcaacagcac gttgcagcat gtcttctgtg ataggggtgg
     3361 ctaccgggta gaaaccagcc agtgcgataa cgtcgctgaa ctccagatca tccagtgtca
     3421 ttgccgctga catgttttca gcttcagttg ctgtatcccg acattcctgc actcgtgaaa
     3481 tcgtgtcagg atgcataaca atacctgatg ccattgtgcg gataagacgt tcaagcagcg
     3541 cattatgttg tgccagaagt tgattattaa gttcgagact ggtttctaaa ctcatactgt
     3601 ggtcctcgct acaaggagaa tgaaagtgat gatcagaccg agcgcagtgg caacggccag
     3661 accggtcatc aaatcgaagt ttttacggcg ataacggaga acatcgcgcc ccgtcagtcg
     3721 atggatgtgt tcaggtttca tcggttgtat tccttttttc atatcgggga gcgcgctgtt
     3781 gcgagtgcgc tttcagacat aaaaaagccc gtcacttgag gcgggcaaag gctacacaca
     3841 gcaattacat agatgattca gttggtattg tgcgtacgag gtaccacaag gttgaagcgg
     3901 acacgccagt acgttgcaat ggcgtacagg aaagcaccac attcagggca gtgctcataa
     3961 cattcgttga tttggtatcg ttcaggcttt ggtgcctgaa gttgtgcttg gcaagcaggg
     4021 cattcacctt tgagcggttt atatgttttc acaccgtttc ctcattcagt tctctttggt
     4081 ggttcggctg ctgcggtctg agcgttttac ttgtgcatga tgttttcctc cattggttca
     4141 gacgccccat catcggggcg tttcaatttg cgtgacttat cagctcgtcg cggtgttgtc
     4201 ctctacgctt accgtacgca tacggactcg gcgcttacct cgatcccatc gggtgctatt
     4261 tcgttttgcc aggagtactg cggcttacct gtcacgcggt tcagtttgtt aaagagcgat
     4321 tacttattgc gattaaaaat acaagcaaac ctgtaaacgc gtcaacaggt gttttggtaa
     4381 aaaatgcatt attaacctaa ttacttgttt ttacaggtaa taaaaaagag ggcctaagcc
     4441 ctctttgttt agtagtcagg atgcgattat ctttttctgc ggaatatgcg atgttctacc
     4501 attgtcccta tgatcttaat tggtttttcc caggagcgac aaatagggta atcactattc
     4561 aatggaacaa gttcaaagtc ttcctcaccc ttagcgccaa tccctaccgg gcggtatttt
     4621 ttgaacgttg cttcgtggct accgttacat gccgcaacga attcaccagg agtagggtat
     4681 acgtcagtgt caattataac gatgtccccc tctttaaatt caggttccat tgaatcccca
     4741 cgtatacgta gcgcaaatga attttctgat agctcagccg tggtgagaat gtattcgaaa
     4801 tcaccttctt cacgaagtag ctcagcgctt gtgaaagccc ctgcttgtac gtagcttaga
     4861 acaggcaccc ttcgggctcc gtagttgaaa tctgtaacaa catcgccccc aagaagtagc
     4921 catttaggat cgcattcaag agctgacgct aaagcaagaa gattgcgtgg tttaagtgtt
     4981 ttcccgttct ctatagcttc aatcgactgc tggctaatgc ccgctcgttt cgcaacttcg
     5041 acctgagtta agtttagttc tgttctgcgt tttttagctc ggttggcaag attcatgttg
     5101 actccttttc tgtcattgat gattacagat acttctgtat ttgacaaaca aggttgcctg
     5161 tgaggtaatt acaagtaaac ctgtaataat gagggcttac tcaatgacca gttctaccct
     5221 tgcttcacgt ataaaggagc gccgcaaagc tttaggtata acccaaactt cattggctga
     5281 aagtgttggc atgcgccagc agtctgttca ataccttgag tccggacgcg ccacacgtac
     5341 cggtttcatc cttgaattgg caaaagttct gaaatgcgat cctgactggc tattaaacgg
     5401 cgaaaattca gagcagcaaa aggtgtaacc catgcctgat aacaaaccct ggggagctac
     5461 gcccgatgag tggttccact tcgatctggt attggggatg acagacaggc ttttacctgt
     5521 ggtgtccaat ccctgtgcag aactgtcgcc taatagcaag ctgtctcata atttcggcaa
     5581 aactcccagt cgctacaatg gccaacgtaa ggttgttggt atccccgact gggctaacta
     5641 tgttgttacg gaaaacgatt ttgcccggtg gtcgaacgaa cccgattacg gtatttgcgt
     5701 tcgtactggc gacggggtgg tggctctgga ctgcgatatc aatgacgccg ggatgcagga
     5761 gatcgtacgc aatattatcc tgtcatgcct gggggaactt ccgccacgtc gctggcgggc
     5821 tgacagtcat aaatgcctgt acctgattgc tgttgatggc gactaccgta aacgtggtca
     5881 ccgcttggaa ggcgaaaaca aacaaataga gctgctcgct aagggccagc agtttgtggc
     5941 ttgtggtacc catccggcag gcgaacgtat tcagtgggac tgcggtttgc ccggtgaacc
     6001 tctgaaaatt acatctgaac agctcgaaag cctgtggcag cgtctggcgg ataacttacc
     6061 cgtgaaagat agctataccg caggtgcggg acgtcagcgt gacctttcct gtgttgatcc
     6121 ttccgcgaca gatgatgtcg ccgactggct tgacgcgaac gggtggactc tgtcggtcag
     6181 ctctgatggt tcccgcaatc tgaaaccgtt tcggtatgag tcagaataca gcaacggttg
     6241 cagcgaaaca tccattaagt atttcccaaa agggacgggg ggatttgaac agggacattt
     6301 taaaagcatg cacaacaccg atgcggggct tactgatgcc gactggctgg aaggctacgg
     6361 ctatacgcaa tccctgttcg aagatctcac ggtggctgaa gatggcgata agcctggatt
     6421 taccgatatt aatacagata tgacagctca ttttctggaa cgctttatct acgtcatcga
     6481 gggtgaccag gtatgtgatc ttagccgacc gccatatcag tgcatgatgg atatgaagtc
     6541 gttcaaaaac ctgatggcgc cttaccagtt cccgccagaa gggaaagggc aaccaattcc
     6601 cgcaaccaaa cggtggatag agcatcggca taagaagatt gctgagacga caggctataa
     6661 gcccggtgcg ggacgtatta tcgagcgttt cgacgggcgg tttgagataa acgaatttta
     6721 tatgcctgag catccacgta cagcggatac aagtaaggtg tccacgttcc ttaatcatat
     6781 ggcgtatctg gtacctgatg cctggcagcg cgagtttttc atcgcccgtc tgggatggat
     6841 ggttcagcgt ccggagcgtc gatgcccgat ttctattctg catgtggcta ccgcacatgg
     6901 tacgggcaga ggatgggtca gccagttaat ggagcgcgca cttggtccgt ggaactgtgc
     6961 tcgtactcgc atgaagatcc tgtgcgataa ccagttccat gactatctgt acaatacgct
     7021 tctatgcacc attgacgagg tgcgcgaaaa cgataagcgg tatgaggtga acgataagat
     7081 ccgcgatgta ctgacagaac cacggtttga agtgaaccgc aaatatggca gcaaaaaaac
     7141 gatggatata tacactggct ttctgttcta caccaaccac tttgatgcgc tagcgctgcc
     7201 agaggaagac agacgtatcg ctgtgcttgg tggccctgac ttcgccgcaa ctgaagagca
     7261 ttacgccagc ctgtacggtg cgctaagcga cagcgacttt atcgcccagg tgtactggta
     7321 tctgatgggc gttgacctgt ctcgctttaa ctggcagcgc gcccccgaga cgaaagaacg
     7381 ccttctgatg attgaaagca ataagagcga tgtggaggct gctcttattg aaatcctgga
     7441 taatccgcct gtgccagcaa tgacttatca acaaattgtt aacgcaatac tggcagaagc
     7501 aggaatggat gtagaaatta accagaagca cataacaaag ctattgcgag aaaaaacaaa
     7561 acgtgaagca gtgaaactaa aagtggacgg catgactgtt cgcatgtggt tacttgtaaa
     7621 aaataccgat ttcagcaacg aagaattgcg tgaaatatat aaaaattgcg aaattttgca
     7681 atctggattg taaaaaggtg gcagtaaggt ggcagataaa cgactaactg ccaccaggta
     7741 aagatcaatc aaatcaattg gaagcggtga aaaggtggca
//
LOCUS       DAZFRN010000202         7768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFRN010000202 DAZFRN010000000
VERSION     DAZFRN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.204"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(29..832)
                     /locus_tag="ACHKUB_004527"
     CDS             complement(29..832)
                     /locus_tag="ACHKUB_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6657448.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSPQLPVVIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVVAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNR"
     gene            complement(833..1138)
                     /gene="ccdB"
                     /locus_tag="ACHKUB_004528"
     CDS             complement(833..1138)
                     /gene="ccdB"
                     /locus_tag="ACHKUB_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO6657449.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1140..1358)
                     /gene="ccdA"
                     /locus_tag="ACHKUB_004529"
     CDS             complement(1140..1358)
                     /gene="ccdA"
                     /locus_tag="ACHKUB_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO6657450.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2205..3443
                     /locus_tag="ACHKUB_004530"
     CDS             2205..3443
                     /locus_tag="ACHKUB_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFO6657451.1"
                     /translation="MEVFMKDVSILNEMIFSASTENELQEKFKDIYHRINNESEYDDS
                     GSVEFLPLPRLNSRSERSEVQDIISVVIESGFFTSGPYIGKVEETLRLFYGAHTCIAT
                     SSGTDALKIALKSVGVEPGDEVIVPLNSFAATENAVMAVGAVPVFANIDSSFNMLPDE
                     VDRLRTSKTKAVLPVCLYGSCMYIDAIHRIARKADIPVIVDAAQCFGIRSLINHCDLL
                     ALSFNPFKNIGSLGKSGAVLTLSPELARLARQYSYHGFAEGKKNIKAQNWGLNSRMDN
                     LQAATLSVKLHHFENNAKKRCLLAARYHLLLSDLSHNIILPTETHQNTWHLFPILLCK
                     GERDSLFAFAREKGVELDIYYPVLSHCGEHPLATGYSRREQFSDSEKIHSALLHLPLH
                     NHMSLQEQNIVIEVLHDYFK"
     gene            3427..4257
                     /locus_tag="ACHKUB_004531"
     CDS             3427..4257
                     /locus_tag="ACHKUB_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HFO6657452.1"
                     /translation="MTILNKDWPVEFPFPTVIRTVVCSDLDETYIPSDSDKKKLSGIV
                     QLESYITSHAEKKGILAGWITGTNLDSAWRKSRGYISRSPHFICCSLGTEFYWVKNGR
                     LIPSETWAERISRSGYSRQNVNCLVKILMEKEIPLLKQPEDYQGPFKVSYYYPESPSI
                     ASDFATIQELADERRIRVVFTRCNPAAGDPSGCYDVEFIPLCCGKDQAVSFLMEETGL
                     PKEAIIAFGDSANDFAMFAQAGSGYLVGNADPFAVSQYGNRLNSAYCHGILSVLKELQ
                     "
     gene            4257..5273
                     /locus_tag="ACHKUB_004532"
     CDS             4257..5273
                     /locus_tag="ACHKUB_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6657453.1"
                     /translation="MIIGIVGCGGIARAHVNALCLNPLVTGLALYDVNPDKVRQLSEI
                     SSLPVTNCQDLEQLAGVSQGFIICTPNHLHVSVAEEVLRYKQLPFVCEKPLSTDLNSA
                     RYLQEIAPKGSIVSFNYRYNPVIDMIMRLRNERKLGELHFFSAEFNKNSALTRHHLTW
                     RDSAQQSKSSGALGDLSCHLLDLFCFIGESPVVVHGIKTVKGTRVGTKSDGQVEVDDN
                     GYVMGSSEKGAYFRVHASKSETDQNLGLHIQLVFENGEIRYSTHHENRLLLILFNDTN
                     TETIGFDALKRLPDPPRELPFWSDSFIHLHDDWCALIKYGHSSPKLADLSSGLHIQEI
                     IEAF"
     gene            5275..5544
                     /locus_tag="ACHKUB_004533"
     CDS             5275..5544
                     /locus_tag="ACHKUB_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HFO6657454.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            5541..7073
                     /locus_tag="ACHKUB_004534"
     CDS             5541..7073
                     /locus_tag="ACHKUB_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6657455.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWTPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFPGVEQVFRYLSAVAGEDALRESTRLNTEVV
                     SLRKDAGHWVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYRASGLLPDMANNNAVHVNEKIIPNVAA
                     GLIKVKPLAERFTGEGAIEFVDASQEKYDVIITCTGYEMPDYSFIQGFDRTQLYEHFF
                     WTEDPSLAVINPPVDTAGFGAAFPYFDIISQWVMNVFSGKTSLPEKEAMRKWCAEHMA
                     SLHVKRFYDSWLETIRIGLLSGLLPDPARDFSRYWNIISSMVKPAYLATPPAFPEHGM
                     MDSLFDFRIARIRILSGLGNDALGYLLKKGDITDAEYRAALEIDPRQSISVHLPYSQT
                     YL"
     gene            complement(7106..7558)
                     /locus_tag="ACHKUB_004535"
     CDS             complement(7106..7558)
                     /locus_tag="ACHKUB_004535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6657456.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISIILGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPVENKGIYAGNPARLIRIL"
BASE COUNT         2042 a         1907 c         1792 g         2027 t
ORIGIN      
        1 aatcacaatt cataattatg atttatgcct acctgttttt cagcattgtg acagcatcgg
       61 actcaggcat cgaaaactgc acccggtgcc gtgcagccac atccagtgca aacaccttcg
      121 tgtagacctc cgttgagctg atggacttat gccccatcag gctctgcagg accttcagcg
      181 gtataccggc atacagcatg tgcatcgcat aggaatggcg gaacgtatgt ggcgtgaccg
      241 ggacagagaa cgtcacaccg tcagcggcag cggcgacaac cgcttcccca atccaggtcc
      301 tgaccgttct gtccgtcact tcccagatcc gcgctttctc tgtcctgcct gttcgtttat
      361 tacgccgttc catggggatt ttcagtgtgg ccaccatcgt ctgcagctgg ctgacgtacc
      421 aggagtcgga gagcggaacc agccggtgag tctgctgacc ggcgggcatt cttcctgccg
      481 tcctggcggc tttttcggtc cgttgtttca gggtcgcaag ctgcacaaac ggatacggag
      541 gcgcgagcga aaaatccccc cgcgtcagcg ccagtgcttc attaatgcgc gcaccggtgt
      601 tccacagtgt ggccaacagc atcttgcggc gcagatccgg gacgtaatgg agcagggcac
      661 tcacttccgg agccagcaga tatttcggca gttcatcatg aaccatcgac atctggcgaa
      721 gtgccagagc tgccggatag tcaatgacaa caggaagttg tggagactgt ccggcagaat
      781 acactgccgg taccatggct gcagactggc tgtgtataac ggagcctgac atttatattc
      841 cccagaacat caggttaatg gcatttttga tgtcattttc ccggtggctg agatcagcca
      901 cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc
      961 tttcatcccc gacatgcacc accgggtaaa gttcacggga gactttatct gacagcaggc
     1021 gggcactggc cagggggatc accatccgtc gcccgggcgt gtcaataatg tcactctgga
     1081 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt
     1141 caccagtccc tgttctcatc ggcaaaagag ccattcattt caataaacct ggcgacctct
     1201 gccatccctt cctgattttc agcctgccag cgttcagcac gcagtcgccg ggcttcgttc
     1261 tgcatggtcg tactgaccag accggagata ttcacgtcat acgccttgag cagctgatag
     1321 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag cacacctctt tttaacatac
     1381 ttctggtatg catatcagta tatattctta tgccgcaaaa atcagcgcgt taatacacat
     1441 actgttatct tgcttttagt aagccttatt tattttacct tgcattatgt taaccaataa
     1501 aaattaaaat cagacttata aaaacaaagc gtaattatcg aaggaccatt tccggcagat
     1561 ccgatttttc tgttattgac gggtttccgg aagtgtacca cgctgaggaa atcatcatat
     1621 cgctgttttc cgcccctgcg tggcgcatca caggtcaggg aaataacaca acatatagtg
     1681 tcatgagcag ccgaatgaag cactacatgt tgtacttaca ggaggtggcg ttgtgtgggg
     1741 gagaggaaga tccgtgacca ggggttttcg gatctctgga gcagaaaaga aaattattgc
     1801 cagataatca caaaaaatgg tggattattg tgatgaagtg attattgcaa tcagaacaat
     1861 aactggaatt gtaaagatca tattcatcaa taaatagcag agtcacagat ctgctgaaag
     1921 ccaaaccatg aagatactac ccgagtccgc ttctggaaga tccatttcaa atatccggat
     1981 acacaggcaa ctgtggcgac gattgtaatg acattaacca tatcggcttc cctgaagcat
     2041 tggttatggc aaccacgtta tcaatatctt tccatcattt tcagtaaata tcactaacag
     2101 accgatatta ccggacctgt aagttatttg tgactgatat caccaatttg taaatcaaat
     2161 gtgaaaatat ggtttatctg tgctttattt ttccccgaca taaaatggag gtctttatga
     2221 aagatgtcag tattttgaat gaaatgatct tcagcgcatc aacagaaaat gaactacagg
     2281 aaaaatttaa agatatatat caccgtatca acaacgagag tgaatatgat gattctggtt
     2341 cagtcgaatt tctgccactt ccaagactga attcccgttc tgaacgctca gaagtacagg
     2401 acatcatttc agttgtaatt gaaagcggat tttttaccag tggcccctat attggaaaag
     2461 tagaagagac attaagatta ttttatggtg ctcatacctg tattgccacc agtagcggca
     2521 ccgatgcgtt aaaaatagct cttaaatctg ttggagtgga acccggagat gaggtcatcg
     2581 ttccgctgaa ctcctttgca gctacggaga atgcagtgat ggccgtggga gccgttccgg
     2641 tcttcgctaa tattgacagt tctttcaata tgcttccgga tgaagtggat cgcctcagga
     2701 cttcaaagac aaaagccgtg ttgccggtct gcctttatgg ctcatgcatg tacattgatg
     2761 ccattcaccg gattgcccgc aaagccgaca ttccagttat cgtggatgca gcacagtgct
     2821 ttggtatcag gtcactcatc aaccactgtg atctgctggc gttgagcttt aatccattca
     2881 agaatattgg ttctcttggt aaatccggtg cagttctaac cctttctcct gaactggcac
     2941 gcctggcaag gcaatattca tatcacggat ttgctgaagg taaaaagaat attaaggccc
     3001 agaactgggg attaaacagc cgtatggata atctgcaggc tgccacgctt agtgtcaaac
     3061 ttcaccattt tgaaaataat gcaaaaaaac gctgccttct tgcagcccgt taccatcttt
     3121 tactgtctga cctgtcacat aacataatac ttccgacaga gacccatcag aatacatggc
     3181 accttttccc tattctgctc tgcaaaggcg aacgtgattc tctgttcgcc tttgctcgtg
     3241 aaaagggagt ggagttagat atttactatc ccgtcctttc acattgcgga gagcatccat
     3301 tggcaaccgg atattcccgt cgagagcagt tcagtgattc tgaaaaaata cattctgcat
     3361 tacttcatct gccgctgcat aaccatatgt cgctccagga gcaaaacatc gttatcgagg
     3421 tactgcatga ctattttaaa taaagactgg cccgtcgaat tcccgttccc gacagtaatc
     3481 cgcactgtgg tgtgcagcga tcttgatgaa acttatattc cttcggacag cgataaaaaa
     3541 aaactgagtg ggatcgttca gctggaatcc tatattacat cccatgccga aaaaaagggg
     3601 attctcgctg gctggatcac aggaacaaat ctggattcag cctggcgaaa atccagggga
     3661 tatatatcgc gaagccctca ctttatatgt tgcagtctcg gcaccgaatt ttactgggtg
     3721 aaaaatggtc gtctcatccc ctctgaaacc tgggcagaaa gaataagccg ttcaggctac
     3781 agtcgtcaga atgtcaactg tctggttaag attctgatgg aaaaagagat cccccttctg
     3841 aaacaaccag aggattatca gggccccttt aaagtgagct attactaccc tgagagccca
     3901 tctattgcca gcgattttgc caccatccag gaactggctg acgagcgacg aattcgtgtt
     3961 gtgtttaccc gctgtaaccc ggcggctggc gatccctctg gctgttatga cgttgaattt
     4021 atccccctct gttgtggcaa ggatcaggcc gtgtcttttc tgatggagga aaccgggctg
     4081 ccgaaagaag ccattattgc ctttggtgac agtgcgaatg attttgccat gtttgcacag
     4141 gccggaagtg gctatctggt gggtaatgcc gacccgtttg cagtcagcca gtacggaaac
     4201 agactgaaca gtgcttactg ccacgggata ctgagcgttc ttaaggagct acagtgatga
     4261 ttatcggaat tgtcggctgt ggggggattg cccgtgccca tgtcaacgca ctttgtctca
     4321 atccgctagt aactggtctg gccctgtacg atgtgaatcc agacaaagtc cgacagctca
     4381 gcgaaataag ctccctgccg gtaacaaact gccaggatct ggagcagctt gccggagtca
     4441 gtcaagggtt cattatctgc acaccaaacc atctccacgt tagcgtggca gaagaagtgc
     4501 ttcgctacaa acagctcccg tttgtgtgtg aaaaacccct ctcaacagat ctcaactctg
     4561 cacgatatct acaggagatt gctccaaaag gtagtatcgt gtcttttaac taccgatata
     4621 accccgtcat cgacatgata atgcgtctga gaaatgagag gaaattaggt gaactgcatt
     4681 tcttctcagc agaatttaat aaaaattctg cactgacccg ccatcacctg acctggcgag
     4741 actccgcaca acagagcaaa agcagcggtg ctctgggcga tctatcctgt catctgcttg
     4801 atttattttg ttttataggg gaaagcccgg tagtggttca tgggatcaaa acggtaaagg
     4861 ggacacgggt gggtacaaaa tcagatggtc aggttgaagt ggatgataac ggctatgtaa
     4921 tgggcagttc agaaaaggga gcttatttca gagttcatgc cagtaaatcg gaaacagatc
     4981 agaatttggg actgcatata cagcttgtat ttgaaaatgg tgaaatcaga tattcaaccc
     5041 accatgaaaa tcgcctgctc ctgattctat ttaatgatac gaatacggaa acaattggct
     5101 tcgacgctct gaaacgcttg cctgatccac cacgggagct tccattctgg tctgattcgt
     5161 ttattcatct tcatgacgac tggtgtgctc ttattaaata tggtcactcg tctcctaagc
     5221 tggcggacct gtcatccggc cttcatatac aggaaataat tgaggcattc tgatatggat
     5281 acaattcttt taaccgggct gtttgctgca tttttcacaa cgtttgcgtt cgctccccag
     5341 agcataaaaa ctatcaggac acgcaatacc gaaggtatct ccgttgttat gtatatcatg
     5401 ttcctgacag gtgtaatctc atggatagcg tatggcatta tgcgatctga ttttgccgta
     5461 ctgatcgcca atattgtcac actgtttctg gctgcaccag tgcttgtaat aacacttatc
     5521 aaccgcagga aaaaacacgt atgaatattg caatcatcgg cgcgggtcct gccggcatca
     5581 tttctgcccg taatgcgatt aaagcaggtc actccgtggt tctgtttgag aagaataccc
     5641 gaattggggg gatctggacc ccctggagtg gtggcgctta ccgtaatgcc tgtatgcaga
     5701 actcccgtta tacatttcat tatactggct ttccccctgg cgatatcgat gaatttccag
     5761 gagtggaaca ggtattcaga tatctttcgg ccgtagccgg agaggatgcc ctccgtgagt
     5821 cgacccgact gaatactgaa gttgtttcac tcagaaaaga cgccggacac tgggtgatcc
     5881 gctgcgcttc tgaaggaaaa gacacggaag acatttttga ccgggtcatc attgcaacag
     5941 gtgaactctg gcaaccccgc cggccccccc tgccaggtga ggaaaacttc tccggaacgt
     6001 tgatcacgtc gagagattat caggagccag aggcatttaa aggaaaaaat atcctcatca
     6061 ttggcggcgg tgtcagcggt gcggatattg cctcagacct tgttcccttt gccagaagcg
     6121 taagtctgtc cgtcaaaaag atgggacttt atctgccgag acaattcccg actggcccga
     6181 atgacatgat gcactcctat ctgggcaggt gtctgctgag ccaaatgaat tatgaagatt
     6241 ttatcggtta tctcgacacc atgatgcctg actacatgca ggcctaccga gcatccggtc
     6301 tgttgcccga catggcgaac aataacgcgg tacatgttaa cgaaaaaatc ataccgaatg
     6361 tagccgccgg tctgataaaa gttaaacccc tggcagagcg tttcaccgga gaaggcgcta
     6421 ttgagttcgt cgacgcatct caggaaaaat atgatgtaat cataacctgt acaggctatg
     6481 aaatgcctga ttactcgttc attcagggat ttgacagaac acagctctat gagcactttt
     6541 tctggacaga ggacccttct ctggccgtca ttaatccccc ggtggatacc gccggtttcg
     6601 gggctgcgtt tccctatttt gatatcatca gccagtgggt aatgaacgta ttctccggga
     6661 agacttccct tccggaaaaa gaagctatgc gcaaatggtg tgctgagcac atggcttcac
     6721 ttcatgttaa gcgattttat gacagctggc tggaaaccat ccggattggg ctgctgtcag
     6781 gtcttctccc ggacccggcc cgcgactttt cccgatactg gaacatcatc agcagtatgg
     6841 tcaaacctgc ctatctggcc actcctcccg catttccgga gcatggtatg atggacagcc
     6901 tgtttgattt ccggatcgcc agaatacgca tactgtcagg gctgggaaac gatgctctcg
     6961 gttatttact gaaaaaaggt gatatcactg acgcagaata ccgggcggcc ctggaaatcg
     7021 atccccggca gtcgatttct gtacacctgc cgtattcaca gacctacctc tgataaaaag
     7081 ggccagtaac ggcccttttc ttttttcata atattctgat cagtcttgcc gggtttcctg
     7141 cataaatgcc tttgttctca accggtttta ctacaactgc ccccgcaccg ataacagctc
     7201 cgctgcatat atacggagac agaataatgg ctccactgcc aacagtaacc gaatccccca
     7261 ggataatgct gatccagtta tccggtgacg gatctggtgc tcctgaacga aacagatcgt
     7321 tggcgaacgt cacgttatga ccaatgaagc agttctcccc cagtgttacg ttctcacaaa
     7381 taaacgtgtg cgactgtatc cgacttccac taccaatgac acagcctttc tgtatctcaa
     7441 cgaaggggcc gacaaacaca ttgtccctga gttcacattc ataaatgttg accggtttga
     7501 caatacggac attggtccca taaatcacat cccggacagc acattccata aatttcatac
     7561 tcacacgtcc tttaaataca gatgacactt gccggaatta tacagcccag attcagttgc
     7621 catgtttcag aatagcttat aagccgcact tttaccgata ctcagcaaca aatcaggtct
     7681 ggatccactt cagccggaaa ccggatctgc gcggcgggaa acgggcaggg gatatgataa
     7741 ttaagcaaca cacataaatt catatcag
//
LOCUS       DAZFRN010000203         7668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFRN010000203 DAZFRN010000000
VERSION     DAZFRN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.205"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            248..462
                     /locus_tag="ACHKUB_004536"
                     /pseudo
     CDS             248..462
                     /locus_tag="ACHKUB_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388349.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(987..1244)
                     /locus_tag="ACHKUB_004537"
     CDS             complement(987..1244)
                     /locus_tag="ACHKUB_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657457.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(1298..2065)
                     /locus_tag="ACHKUB_004538"
     CDS             complement(1298..2065)
                     /locus_tag="ACHKUB_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6657458.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPALLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            complement(2062..3120)
                     /locus_tag="ACHKUB_004539"
     CDS             complement(2062..3120)
                     /locus_tag="ACHKUB_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6657459.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            complement(3139..4137)
                     /locus_tag="ACHKUB_004540"
     CDS             complement(3139..4137)
                     /locus_tag="ACHKUB_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6657460.1"
                     /translation="MKLSRLFPLLALLALLLAASLHAENTHKEVRIASPWPAQNTIIA
                     MLGYGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVP
                     QNMVVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRN
                     VALVTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQ
                     NRSGEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWC
                     RETSEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            complement(4148..6313)
                     /locus_tag="ACHKUB_004541"
     CDS             complement(4148..6313)
                     /locus_tag="ACHKUB_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6657461.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVDDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSIGHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            6796..7605
                     /locus_tag="ACHKUB_004542"
     CDS             6796..7605
                     /locus_tag="ACHKUB_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657462.1"
                     /translation="MSLPVPFGAIVTVVKKCWSTLTQIMLIKTYQLSHERWAVRHRLG
                     ASWHSLGNYLEYSLRFAQLTDKEPRCSRIAFRSKEERLQQVELVFEAIGSGLRYQETL
                     CIHDVNNSPIILTLSNIPTLDVLILDDGFAFTVEQYQLKYCTIKLMTGESISIDNSPK
                     YSLMQNWCLNDTWKRRWGIIWNCNAIECARRRIAEYWKWGFCLPLVGRPPLYSPSRKG
                     IHLFEAKPLRWFMTRPWCLNIQFWTAIWSGLFILSDENVLQWRWKEPPQQD"
BASE COUNT         1909 a         2013 c         1934 g         1812 t
ORIGIN      
        1 tcagtcaggc ttaaaaagac aataaataca gtgagttaaa tacacagaga aaacgaaaac
       61 gtagtatcag agggtacatc ttacaacaga gactgaattt ccggtttttc ataaccggca
      121 cattccggaa agagcaggct aaccggaggc tttctgacac cagtccctgg cagtttaatc
      181 tgtacatcat gtacggatgt tttgttcgca tttttaaaat cggcaaaaat gcgagaagag
      241 cagctatatt gtggatgaac tgaaaagtgc ccgggagcaa caaaaaaggc gtgatgaacg
      301 tgctgatggt tccgttccgc tgaaactgtc cggaaaaatc cacaaacagc ctgaaagtat
      361 tgcccgaagt cgtggtgccc cccaaaaaaa ctgttgaatg aaatgattga gctggcgtac
      421 caggattttg tggcaaatga gcagcacaag actcggagtt aaagactgca gggccccggt
      481 taccaattct gcagcataca caacgaatgt atccggctct tacctgtgag gggcacgaaa
      541 attatacatt gagcatgagt ggataatccc gggtgagagt ctcccgtttt acactattca
      601 ctaaaggtga ggcatatcat tcagtctctc tgaattcttc atttcagtaa cttcagctac
      661 cttcgtttca tctcccccac gaaccggggc cgcagcttgt tgtatcaata ccaaattgtt
      721 ggtcattact gtcctgtatc actgggctgg caagtgaact gaaatccgca ccttaatatt
      781 ctgactggct gcgtacattc atggcatgaa gcggccggaa cggctgagct gaaggtccgt
      841 tgtgagcgaa cagcggacat tcactacaca tggttcaaac atgaaccgga gctttattgt
      901 ctagctgtca gtgtactgcg atttacccgc tcggttgtcc agaaaatcgg tctgaaatcc
      961 agtcattaaa gggccttagc ctgacattaa gcaacttggt aggcggatag ataatagaaa
     1021 taggaatgcg caggtctgtc atgttgccta gaacggtgac aagctgtccg gtgcgaaatc
     1081 attccggcag cgacaggctc atgtgtcagc ccgacgccca gaccgtcaac gagcatgtgg
     1141 atctgcgagg cgttatcgtt cgcagcgatg ttaaaacgaa caatctgctc atcttctaga
     1201 agcacaagtg ctttaatgac attaaggcgg tctatatgtt gcattacaga cttcctattg
     1261 atctttacct gaggctgaag caaccacgtt aatccgacta tttttcgatt gcaccatcca
     1321 ctatcaccat cgatcttccc tgagcacaac gttcaactct cccgcgtacg ccgtatacct
     1381 gcgcaaggct ctctgcatgg atcaccgttt gcgggtcgcc gctgtcgata agtttcccct
     1441 ctttcagcat gatgacctgg gcggcatgcc gcagtgcgat attgatatcg tgtaagacga
     1501 ccagcgtaac catattgcgt cttcgcgttt cgcgcgatac aacatccatc acatggaact
     1561 gataattgag atccagcgcg ctcagtggtt catccagcaa taataatgcg gggcggcgaa
     1621 taagcgactg tgcaagcccc accagctgct tctgaccgcc ggacaggcta tcgaggtagt
     1681 tcattgccag atgtgcgatg cctagctctt cgagcagcgc tatagcctgc gcctgatttt
     1741 gcccggcgcc ggaggcgcgc tgggcgacaa ccaccgactc cagcacctgt aaatgcacgc
     1801 cctgcggcag ggactgcggc aggaacacaa ctttttcagc ccgacgagca aacggcagcg
     1861 ataagagatt ctcttcattc agccaggctt cgccactggc gcgatttagc ccggccaggg
     1921 cgcgcagtag cgttgattta ccgcagccat taggccccag cagtacagtg acttcgccgc
     1981 gcggcagcgt agaaatcgac agatgctcaa taatccgccg tttgccgtaa ccggcgcaca
     2041 gcgcattaat cgtaagcccg ctcatgacat actccctcga tgacgcatta caatgctcag
     2101 aaagaacgga acgcctacca gcgaggtaac aattcctacc ggtaaaatga caccgggaat
     2161 gatgttcttc gacgctaccg aggccagcga tagcaccagc ccaccgataa ggatgcttcc
     2221 gggcaaataa aagcgatgat cttctcccag caacaggcgg gaaatatgcg gtgcaaccag
     2281 gccaataaag ccaattggcc ccacaaacgc aaccgaaagg gccgccagca ggctgatgcg
     2341 cagcagggaa cccaggcgca aacggcgaac atcaatgcca aaactcatcg cccgctcttc
     2401 gccgaggcgt agcgcggtaa gctgccaggc acgacgcagc gaccagggta aaacaatcgc
     2461 catcgccgcc gccagcaccg ccagtttctc ccatgatgcg cgagccaggc tgcccatcgt
     2521 ccagaacacc aggccctgca aagtatcttc atcggcgaca aactgcataa tggacaccag
     2581 cgcattgaag gtaaacacca gcgcaatccc aaacagcaca acgcccgacg tcggcacgcg
     2641 cgtccagcgg gtcagactat cgagcagcag ggcggacagg agcgcaaaaa gaaaagcgtt
     2701 tgccggtata aacccgcttt cgggaacgcc gggaataccg atgcccagaa caatcgccag
     2761 cgctgcgcca aaggccgccg ccgacgaaac gccaagcgta aacgggctgg cgagggagtt
     2821 attcagaata gtttgcatct ctgccccggc caaacccagc gccattccta ccaggagcgc
     2881 catcagcgca tagggcaaac ggatgtccca aacgataacc cgcatgccgt tagtcgcccc
     2941 ggacggatgc atcaaagtct ttatcagcga atgtaaaggg agccctgagg ggccgagggt
     3001 gaaatcgagc agcagcgaag caatgatagc gataaaaata accgccagta ttaccagacg
     3061 ctggcgaaaa agctggcgat agcggttctc gaccgacagc gtactttcca atacagtcat
     3121 ggtttttcac agcctccgtt agcgcggcgg attgagcaca tcgctcatct gcgcatcgct
     3181 taacgtcagg ccaaagaact gccgataaaa ctcacgcgtc tctttgcgta tatccacatc
     3241 agcaaatcgc gcaggataaa gcgttttcgc cagccacaaa aattgcagcg cctcttcgct
     3301 ggtttcacgg caccaccaga acattccttt cggattaacg taaacgcgat gatgaatgac
     3361 cgcgtccacg cttgcccact gcggagaact cagaatggca tccgcgtcgc gcttgttcat
     3421 cgcgatgata accgccggat tggcagtgac aattttctcc agcgcgactt cacccgaacg
     3481 attttgttga tgctcgccaa accaattttc agcgatattt ttgccgccgg ccaggtcaat
     3541 ccagtcctgg tttagcgagg gtctaccggt ggtggtcagc ggattgccca tactgtgata
     3601 gagcgacacg cgctgtgacg caggaagatc tttcaggcgc ccggtgacca gggccacgtt
     3661 gcgatcgaag tagcgttgat aggcgagcgc tttttgctgc gcatcgggcc ccagcaccgc
     3721 tgccgtttgc tgtacgcgct gcgttaacgc ccgcagtgaa ttcgcctcaa acgccagcac
     3781 ctgcacccca gcctgtttca gcaacgcctg ttgaggcact accatattct gcggtacaaa
     3841 aagcatatcg acccccagcg cgataatctg ttcgggattg atttcatgcc cgctgtttac
     3901 gctaactgct gcgactttct ctatgcgcgg caggctctgg cgaaacaggg ggatcctttt
     3961 tgcaatcatc gaggttccga caatgttatc gccgtacccc agcatggcaa taatggtatt
     4021 ttgcgccggc cacggtgacg cgatacgcac ctccttgtgc gtattttctg cgtgcaggct
     4081 ggccgccagc agcagcgcca gcagcgccag cagcggaaat aaacgggaga gtttcatcat
     4141 ttatccatca gaaatcgaag gtgacggatg cacgcacttc gcggccgccg ccgataaacg
     4201 cacttgggga accgttatcg ccatcggtac ccgatgggaa gatagaagcc cagtaatgtt
     4261 tatcaaacac gttgtttacc accacgcgga aagtggttgg gacgttgctc actttcgtgg
     4321 tgtaacgcgt tcccaaatcc catgtggtat agctgctggc gtaagaggtg ttggtatcgt
     4381 tcgccgcgcg tttgcccgta taatggacgt tagcgctgta aacccattcc ggtatggacg
     4441 gcaaactgta ttccgccaac agattggcct gcactttcgg cacgccgaca acctgtttat
     4501 tgctggttga ggcattcgcc gtatctttca gtttcgggtc gaggaaggtc acgccgctgt
     4561 aaatattcag cccctgccac acattcccgg cagcggttaa ctcaaggccg ttgttaacct
     4621 ggttaccctg ctctttatac acgttatccg tatcaagata ggcaaacgga cgttccagtc
     4681 ggaacagcgc ggcgcctaga ttcatctcgc cgatgtccga ttttagcccc acttcatact
     4741 gcttgctgcg atacgggttt agcgtttgac cggcattttt tacgctctca tccgtcggtg
     4801 cggtaccgcc ctgctccagc gaatcggcat agctaacgta ggccattgtg ttaggggtaa
     4861 ttttatacat cagcgcggcg ttcggactta aaccattttc atcaacctgg ttcgtttgat
     4921 taccgtgctt atcgtagttt ttagtctgca gccagctctg gctgagatag aacattgccg
     4981 accactgcgg cgtaaatgtc accgtatcgc cgagcgtaac gctctgctga gtatttacgc
     5041 tggagcggta tcgcggcccg ccggtgcgga ttttgccatc gccctgctca tctatcgcat
     5101 ccgggtgata catatttgtt gtaccccagc tatagctgga acctgttcct ttggcactat
     5161 aaagcgacca gacatatccc gtggtagaaa gtgacagatc gtggccgata gagccggtat
     5221 caatatgacc attcagcccg gcggtgttgc tcaggacgcg aaaacgtccg gcagcggtgg
     5281 aatccttcat cgaacgagag atatcgccct gattgttgag tattttactg gaaacactac
     5341 gcatcgcgcg gtcagcctgc tgccagccca cgccagcgtt catggaccag tcgtcgttaa
     5401 agtagtggat caggcgagtg ctgatggtat cggtagtgag gtcgttgccc gcagtgctga
     5461 gcgccagatt tttgtccttc ggattcggcg cagacggcag tttgacgttc ggcccatagt
     5521 taaagctacc gacatagcct ttctggataa attcgtaatg gctggcgtcg agctgtagct
     5581 gagtgcccgg ctgaatattc cagtcgagcg caacggaaac gagtttgcga cgcagcgtgc
     5641 tgtcatccac attgccttcc ccttcctgat caagcaggtt cacgcgatag ccaaaccgtt
     5701 tgttttcatc aaaatggcca cccagatcgg catggccggt aaacgcactg cggctttgat
     5761 agcccagcgt cactttacgc agcgtctctt cggttgggcg cttcgccacg aaattaaact
     5821 gccctgctgg gctcgccggg ccgtacagcg cgccggtcaa actgttaagc acatccatgc
     5881 gctcaagcat ttccaccgga aacgcggttg ttgaaacgat attcagcccg tccagacggc
     5941 tgttggccac cacgctgccc tgcatccccc gactttgcgg acgcccgaca tccattccac
     6001 cgcgcgcctg catttgcgta ctgggagagt atttcagcat ctcgctgacg ctttgcgcct
     6061 gctgattttc aatcatctct ttagttacag tggtgctgga atatggtgtt tcgatccagg
     6121 atttcgttcc cagcgggccg acatctatat cgttcgtttt gaaaccggca ccggcggcag
     6181 aatcggtggt gtcggcggcg gtgttgcccg tgacaatcat ttcatcttca gaagtagacg
     6241 aggcatgagc atgaagcggt atgaaagacg ctaatgctgg caaaagcgca gcgaaagtta
     6301 tttgagtttt catgaatcac tgatcgttat gtaaatattt atattacgat cggtgcatag
     6361 gcaaaattat gatccagatc acacgtgaat gtaagttatt tacaaattac gctaaccttc
     6421 tgtctccgct ctttgcagca taaaagtatg gcggcgcgtt gttgaaagca ggagcggccg
     6481 ggttgtcaga aagctggttt ggacgcaagt aatatataac aactctgatt accgcatact
     6541 gtcaggacag tatctacttg agcgtccgcc tttggcacag agcggacgat atcactgagc
     6601 tgaaggtccg ctgagagcga ggagcggaag taagcatgta tctgctaatg cgaaaaatca
     6661 ctaacgaaac attggatccc cattgttgcc gatctttagt ggcgttcaag attagcccca
     6721 atcctgatta tcatttgatt acactgactg aaatgatata aaacctaatt atctgatatt
     6781 tgtaggggtt tagtaatgtc acttcctgtt ccttttggtg caattgttac agtagtgaag
     6841 aaatgctggt ctactcttac ccagataatg ctgataaaaa cttatcaact ctcacatgag
     6901 cgctgggcag tgagacacag gctaggggcg agctggcatt ccttagggaa ttatttggag
     6961 tacagtctac gatttgctca gttgacagat aaagaacctc gttgttcacg tattgctttc
     7021 agaagcaaag aggaacgact gcaacaagtt gaacttgttt ttgaagccat tggcagtggc
     7081 ttacgctacc aagaaacact atgcattcat gatgtaaata actcaccgat aatattaacg
     7141 ctttcaaata ttcctacact tgatgtactt attctagatg atggattcgc atttacggtt
     7201 gaacagtacc agttaaaata ttgcacgata aaattaatga ctggtgaaag tatatctatt
     7261 gacaattcgc ccaaatattc gctaatgcaa aactggtgtc tcaatgatac atggaaacgg
     7321 cgttggggta tcatctggaa ctgtaatgca attgaatgtg cccgaaggcg tattgctgaa
     7381 tattggaaat ggggcttttg tctgccgtta gtgggacgcc ccccgttata ttcaccatcg
     7441 cgaaaaggta ttcatctgtt tgaagcaaaa ccgttacgct ggtttatgac acgcccatgg
     7501 tgtctgaata tccagttttg gacggcaatt tggtcaggac tgtttattct cagcgatgag
     7561 aatgtcttgc aatggcgctg gaaagagcca ccacaacaag actaactttc acgaaggccg
     7621 tccgctctga gcgaggagcg gacctcagtg ctggttagat taactagt
//
LOCUS       DAZFRN010000204         7622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFRN010000204 DAZFRN010000000
VERSION     DAZFRN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.206"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..519
                     /gene="ykgH"
                     /locus_tag="ACHKUB_004543"
     CDS             <1..519
                     /gene="ykgH"
                     /locus_tag="ACHKUB_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO6657463.1"
                     /translation="SYSLKINQTTPGILVNCDSNNNFAGQTTMTEDVIQRITTFFHTS
                     PDVKNREIKLEWSGDKRDLPTAETEISRVQASIIKWYASEYHNGRQVLDEIQTPSAIN
                     SELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLV
                     KKMVDEYQQNSG"
     gene            complement(765..1460)
                     /locus_tag="ACHKUB_004544"
     CDS             complement(765..1460)
                     /locus_tag="ACHKUB_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO6657464.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLYNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1453..2880)
                     /locus_tag="ACHKUB_004545"
     CDS             complement(1453..2880)
                     /locus_tag="ACHKUB_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO6657465.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2891..3610)
                     /locus_tag="ACHKUB_004546"
     CDS             complement(2891..3610)
                     /locus_tag="ACHKUB_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO6657466.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4137..4991)
                     /gene="rclR"
                     /locus_tag="ACHKUB_004547"
     CDS             complement(4137..4991)
                     /gene="rclR"
                     /locus_tag="ACHKUB_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO6657467.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPINHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5217..6542
                     /gene="rclA"
                     /locus_tag="ACHKUB_004548"
     CDS             5217..6542
                     /gene="rclA"
                     /locus_tag="ACHKUB_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO6657468.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6651..6887
                     /gene="rclB"
                     /locus_tag="ACHKUB_004549"
     CDS             6651..6887
                     /gene="rclB"
                     /locus_tag="ACHKUB_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO6657469.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6899..7492
                     /gene="rclC"
                     /locus_tag="ACHKUB_004550"
     CDS             6899..7492
                     /gene="rclC"
                     /locus_tag="ACHKUB_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO6657470.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
BASE COUNT         2088 a         1850 c         1657 g         2027 t
ORIGIN      
        1 agttacagcc taaaaataaa ccagacaaca ccaggcatac ttgttaactg tgatagcaat
       61 aataactttg ccggtcagac cacaatgact gaagatgtta tacagcgaat tactacattt
      121 tttcacacca gcccagatgt caagaacaga gaaataaagc tggaatggtc aggagataag
      181 agagatttac caactgctga aacggaaata tctcgcgtgc aggcgtctat tatcaaatgg
      241 tatgcgtcag aataccataa tggcaggcaa gttctcgatg agatacaaac accttcagca
      301 attaacagtg agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg
      361 aacggtgatg gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc
      421 atttgcctgg ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc
      481 aaaaaaatgg tagatgaata ccaacaaaac tctgggtagt aataaacagc attctgatag
      541 agagtgcctg tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg
      601 gatgcctgtc tttttcactg taaatatcct ggcattacta atgccaggat atttacagaa
      661 gagcagcata accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac
      721 ctcgtatgcc ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata
      781 atcagataca ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc
      841 gccgttgaac tagggccgct aatgatgtta atgcaggacg gcattcgttc accggcctgc
      901 gctttctgat gcaatttttc tgcgagttgc gctacacgcg gcaagatagt gcttttacgc
      961 aggataaaaa gagaagattc cgggagcagg ctcaatgaac gcccgcgccc ggcggcggaa
     1021 aaaagaacca cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta
     1081 gcctgctcgg cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg
     1141 cattcctgct gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc
     1201 acgaccgact gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc
     1261 tcgctggtca gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca
     1321 cagcgctgct gttggttaag ttgggtaagc cgctcgttag catagttgta aagcggcgca
     1381 tcttctgctt gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa
     1441 aattcgcctc gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg
     1501 gaaactctct ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc
     1561 aaatttgagt ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat
     1621 catcccgact ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg
     1681 ttgctctgct tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag
     1741 ttttgacagc ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc
     1801 gtagggtaaa tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat
     1861 tggccctgga taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt
     1921 attcatacaa gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac
     1981 ctcagaacgc ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc
     2041 cgcttcgcgc ggtcctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact
     2101 gcgcgccagc atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat
     2161 tcccatcact gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt
     2221 ggtcaccagg cataccgaac ctgtctctgc caccgcgaaa ttacagccgg taatacctat
     2281 ttcagcactg aggaaatctt cgcggatttt ttgccggatg aataaggtca tcgcttcagg
     2341 cgtttccggc ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc
     2401 tttatgaatt gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata
     2461 ttcacccaga tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc
     2521 aatctcttcg gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc
     2581 aacctgtaaa atgtagcggg tagcgtcttc tttggttttt gcaaaccaga cgtgaccgcc
     2641 gttttgcgtc actttttctg agagctggta cagataagcg tcgagattac tcagaacatg
     2701 atcacgtatc tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat
     2761 tttttgccga tttgccccaa tacgctgctg cgcgtttgcc accgctttgc gcatgatcgg
     2821 atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat
     2881 ctttatatcc tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc
     2941 cttcccgttg taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat
     3001 actcagggcg gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt
     3061 cggccatttt gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg
     3121 tcaacagctc cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca
     3181 gcttacgggc caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta
     3241 catcgactac ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa
     3301 ccttttcggc acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta
     3361 cggcataggt gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg
     3421 cgatcagatt tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct
     3481 gaccgcagca tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca
     3541 gcacggagtc tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat
     3601 tgacattcac aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc
     3661 accgggggat caatcatcaa taaatatggc gttctttttt aattggctag caacatacca
     3721 cccctcattt gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt
     3781 ttatttcttt cggcatcgct cacattttat ggggaaatcg acgttcattt ttggcataag
     3841 aaatccattt aaaaaggcta catcccttat acctattcag agctacttat tcaggggtta
     3901 ttgagatata cactgatggt gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat
     3961 agtcggggtt ttatttaatt gaagcaattt agtttgcatt tttatttaaa aactgagcta
     4021 gagtccatca gaaaaaaata tataaagccc aaaccaccgt tggaaataga aatagttgaa
     4081 aggctcacaa ctcccggaat gcggattatc tttgccctga tttctggcgg ttttattcag
     4141 ggtgcaagct gtctgaccct ttcccgatat tctcccggag tacaaccaaa ctcgcggaca
     4201 aacgccttgt gaaaagatga ttcactggca tagcctactg actcagcgat caccacaaca
     4261 gggagcattt cccgggaaaa catctgggcc gctatttgta gacgcaactt tgttaataca
     4321 gccagcggcg tggttccgga aacatcacgg aaaagctggg caaaacttgc ccgagacatg
     4381 tgggcgatgc tggccagcga ttcgacggtc caggcgtgtc ctggcatttc cagcatttgc
     4441 tgtattaccg caccaagacg tggatgcaga agcaaactga gaatgttttt ctctgtatta
     4501 acctgtgcaa tccactcacg caccgcgagg gtaaagaatg tagcgcagat ctggctacac
     4561 agtgcatcca ctcccggcat cgccgatctg gattcctgtt gtaaaaacgg gatcgcctcc
     4621 ctcagccagt tatattccac gctgtggtta atcggtgcta aaaacagcgt ttccggcaaa
     4681 gacgttaaaa aataacgcgc cgaatgttgc aaccgaagag tgccgcagac aatacaggtc
     4741 gattcattat cgacatgact caggcgatga gctgaatttt gtggtagcag gaccacattt
     4801 cccgggcgta atgtaaaaat ctctcccgtc ggcatttcca gcttcgccgc tccttgcgtt
     4861 aacgcatgcc aacgaataac cgataattcc ccggcaccat gtggaagctg ccagtcactt
     4921 cctaacacgc aattcttatc gatcgttcct tgtggagcgt taagcatcaa caaacggcta
     4981 agggcatcca tatcagactc ctgagtggtt tttgattaca aacaccaaca atcttagtaa
     5041 ataactaatc taataccatc aaatggttaa tcatgatttt tgttttgtga ttctgattaa
     5101 gaaaaagaaa ataccagacg ataagacaaa atatcaagcg ttgtgaagaa atggtatttg
     5161 ctcttttgcg tctatagtca tgatgtcaaa tgaacgcgtt tcgacaggaa atcatcatga
     5221 ataaatatca ggcagtgatt attggttttg gcaaggctgg aaaaacatta gccgtcacgc
     5281 tggcaaaagc aggttggcga gtggctctca tcgaacaatc aaatgcaatg tatggcggga
     5341 cctgtattaa tatcggctgc atcccaacca aaacattggt tcatgacgca cagcagcaca
     5401 cagattttgt ccgtgccata cagcgtaaaa atgaagtggt taatttttta cgtaataaga
     5461 attttcataa tcttgcggat atgcccaata tcgacgtgat cgacggccag gcggagttta
     5521 tcaataatca tagcctgcgt gttcatcggc ctgaggcaaa tctggaaatt catggcgaga
     5581 aaatttttat taataccggt gcacaaaccg tggttccgcc aattcctgga attaccacca
     5641 cgccaggagt atatgacagc accggattac ttaatctaaa agaattgcct gggcatttag
     5701 gtattttggg cggcggatat attggcgttg agttcgcctc tatgttcgct aattttggca
     5761 gcaaggtaac cattttagaa gcagcttcgc tgtttttgcc tcgggaagat cgggatattg
     5821 ctgataatat cgcgacgatt ttacgcgatc agggcgtcga tattatcctc aatgcccatg
     5881 tggagcgaat cagtcaccat gaaaatcaag tgcaagtgca tagcgagcac gcccaactgg
     5941 cggtggatgc actgttaata gcttccggtc gtcaaccggc taccgcttcg ttacatccag
     6001 aaaatgccgg tatcgcagta aacgagcgcg gggcaattgt cgttgacaag caattacata
     6061 ccaccgcaga caatatttgg gcgatgggag atgttaccgg cgggctgcaa tttacttata
     6121 tttcactgga tgattaccgc attgtgcgtg atgagttgct gggagaaggc agacgtagta
     6181 ctgatgatcg gaaaaatgtg ccttattccg tattcatgac accgccactg tccagggttg
     6241 gtatgacaga agaacaggcc agagagagtg gtgctgatat tcaggtggtg acattgcctg
     6301 tagctgcaat tccgcgtgcc agagtgatga atgatactcg tggggtatta aaagcgattg
     6361 ttgataataa aacccaacgt atattagggg catcactgct gtgtgttgac tcccacgaga
     6421 tgatcaatat agtgaaaatg gtgatggatg ccgggctgcc ttatagcata ttacgcgatc
     6481 agatatttac tcatccgtcg atgagcgaat cactcaatga tctattttca ttagtcaaat
     6541 aaactcaaaa tcagacgcca gaacaaatat tctggcgtct cagagaaaag aatcttatta
     6601 attccctgtt actctataac ttcttaatca cttcattgat ggtattttat atgtttaaaa
     6661 aatctgtttt atttgcaaca ctattatctg gcgttatggc attttccacc aatgcagatg
     6721 ataaaataat tctgaaacat atcagcgtct cgtcagtatc agcatcaccg acagttctgg
     6781 aggatgccat tgctgatata gccagaaaat ataatgcttc atcctggaaa gtcacatcga
     6841 tgcgaattga taataattca accgcaacag cagtattgta taaataagga tgttcacaat
     6901 ggaaaaatac ctgcacctgt taagtcgggg agataaaatt ggcctgacat tgattcgtct
     6961 gagtattgca attgttttta tgtggattgg gttattaaag tttgtccctt acgaggcaga
     7021 cagcattaca ccattcgtcg caaacagtcc actaatgtcg ttcttttatg aacacccgga
     7081 agactataaa cagtatctga ctcacgaagg cgaatacaaa ccagaagcaa gggcatggca
     7141 aacggccaat aatacctatg gtttttccaa cggtcttggc gtcgtggagg tgattattgc
     7201 tctgctggtt ttggctaatc ctgtcaatcg ctggttaggt ttattgggag gactgatggc
     7261 atttaccacg ccgctggtaa cactctcatt tttaatcacc accccggagg catgggtacc
     7321 cgcattgggt gacgctcatc atggtttccc ttatttatcc ggtgctggtc gcctggtatt
     7381 gaaagatact ctgatgctgg caggtgctgt catgataatg gcagattcgg cgcgggaaat
     7441 tcttaaacaa cgcagtaatg aatccagttc aacgttaaaa actgaatatt gataatccga
     7501 acactgtctg ttgttcactt tttcaggcgt caggccccct ttctgatgat gaagcctgac
     7561 gccatattac cggagatatg acagagtgaa gatcaatatc actcccctgt taaaagattc
     7621 tt
//
LOCUS       DAZFRN010000205         7581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFRN010000205 DAZFRN010000000
VERSION     DAZFRN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.207"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..218
                     /locus_tag="ACHKUB_004551"
     CDS             <1..218
                     /locus_tag="ACHKUB_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6657471.1"
                     /translation="AMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQQ
                     FPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            437..679
                     /gene="yiiF"
                     /locus_tag="ACHKUB_004552"
     CDS             437..679
                     /gene="yiiF"
                     /locus_tag="ACHKUB_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO6657472.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1009..1938)
                     /gene="fdhE"
                     /locus_tag="ACHKUB_004553"
     CDS             complement(1009..1938)
                     /gene="fdhE"
                     /locus_tag="ACHKUB_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO6657473.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSAINPFLFPGEGE"
     gene            complement(1935..2570)
                     /gene="fdoI"
                     /locus_tag="ACHKUB_004554"
     CDS             complement(1935..2570)
                     /gene="fdoI"
                     /locus_tag="ACHKUB_004554"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6657474.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2567..3469)
                     /gene="fdoH"
                     /locus_tag="ACHKUB_004555"
     CDS             complement(2567..3469)
                     /gene="fdoH"
                     /locus_tag="ACHKUB_004555"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO6657475.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3482..6532)
                     /gene="fdnG"
                     /locus_tag="ACHKUB_004556"
     CDS             complement(3482..6532)
                     /gene="fdnG"
                     /locus_tag="ACHKUB_004556"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5945..5947),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6657476.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVLEDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6726..7559
                     /gene="fdhD"
                     /locus_tag="ACHKUB_004557"
     CDS             6726..7559
                     /gene="fdhD"
                     /locus_tag="ACHKUB_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO6657477.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1731 a         1974 c         2042 g         1834 t
ORIGIN      
        1 tggcgatgaa tacggttttt cttcatttat cagaagaggc aattaaacgg ctgaacaaac
       61 ttcggggctg gcgtaaggtt tcacgatctg caattttacg cgaagcggtt gagcaatatc
      121 tggagcggca gcaatttccg gtgcgtaaag caaagggagg caggcaaaag ggggaggtag
      181 ttggtgttga tgatcagtgt aaggagcata aggaatgaaa aaaaattttc attcttgaat
      241 ataaaaaaac agatgccctt attctggtat taatacaagg ctgttttact tgaacttata
      301 ataactgcaa ctgttacatc gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt
      361 aaatgagctt tctttaaaga ttcggcaagg gtacttttct ctccatagat tcaaaaatga
      421 tattggcgag atatttatga actctctggc aggtatagat atgggcagaa ttttactcga
      481 tttatcgaat gaggtgatta agcaacttga tgatctcgag gtgcagcgta atcttcctcg
      541 tgcggaccta ttaagggaag cggtagatca atacctgata aatcaatcgc aaacagcaag
      601 aaccagtgtt cctggcatct ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa
      661 gctgcgcgag gaatggtaat gggaaaaaat gatgtcaatc agattgctga caacgtgcgc
      721 gttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaagtca tgcaaattca
      781 atatattgaa gtttattggt agggctgata agcgcagcgc atcaggcaat catacgcttg
      841 tcatcagtga catacccata accttatcga tgtgttggag ggaagaacga ggcaaacaga
      901 caattgcggc atgatcttga atgggtaacg cggaatacga gagatggtgt cgtcacaccc
      961 tatgagctgt aatccgggcg accagcgcca cccggaaaac gtcagagatt actccccttc
     1021 acccggaaac aggaacgggt tgatggcact gcgggcatag ccttcttgct ccattcgcgc
     1081 gtccagtacc agagaagcga ggtcatctgc cacggcttca actttcggtt ctttttcctg
     1141 ataaagaatc ttcaggtaag tgccacagtc atcgcagctt tcggctttaa tcgcggcctg
     1201 ttcgtcatcc agcgaccagt aatgcagttt gccgctctgc tcgcagttgc tgcattttac
     1261 gcgcactacg tgccattcgg tttcacacag gttgcagtgc aggtaacgca gaccctgagt
     1321 ggtgccaatt tgcaccatgc tggacaccgg catagaacca caaaccgggc aatattgacg
     1381 ttgctcgccg tattcagcgc gggctttgcc ggggatcaga ttggccatct gcgcccagta
     1441 gagcgacagt gcagcccaga taaacggcgc tttatcgctg ctgacggatg agaaatcaga
     1501 ggcaaacagt gcgctggcca tatcttccag ctcctgagtt gatgccttct ccagattctc
     1561 aatcactgcc agcgccgggc cgctcatttc aggtttcagc tcagcaatca gcgccatcag
     1621 cagcttttgc cagtgcttat cacgcggcag aacgtgaata tccagcgggg gcttgccctg
     1681 tgcgctggct tctttaatgc gcgcggtcag atccatctcc agcggatggt cgtacagcac
     1741 cacttcctgg gcgtgggcga taagcgcagc aaagcgcagg taatcaccca gcggattatt
     1801 ttctgccagc tcgcgcagac gctcggcgcg gcggttgtat aaattcttga gccgagggaa
     1861 caataacggc ggaatcatat ccgccgtacg tttctcgctc gaacccagct catcttgcgg
     1921 gattatgcga atactcattc agctttcttt tccgttgtct tgcggacctc acggtaccag
     1981 cgcgggtgat gtttcttcgc ccaggcgctg gtaacccatc cttccaccat ggcggtaatc
     2041 gtgcctttca cccaaagggc ggcgtagata tgcaccatga taaccacaat taacgccact
     2101 gcggcaaatg aatgcagcat taacgcgaat cggatcaccg ggattgagaa agcaggcgca
     2161 aaataaggac gccagataat cacgccgctc accagcagca gaaccaggaa aataatcgcc
     2221 gcccagaaaa cgcatttctg accgaagtta taacgcccgg tgtcacctac ttcctcgttg
     2281 acgacgatct tacgaatatt cttcgcccaa aagatatcat cccgattgat taggttgtga
     2341 tgccagtaac ggaaaaacat gatgatgaac gaggcaaaca taaccacgcc gacaaacggg
     2401 tgcagaattc gcgccagctg cggtgtgccc atgatttgca tcaaccagtt gaaggacggg
     2461 aacaaaaagc ccagcccgct cactgccgcc aggatgaagc agaaggcggt gatccagtgg
     2521 ttgatacgtt ccggcgcggt gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct
     2581 cgtctttctc ttcgtgcaga ttattctctt cctcatccgc acggttcgga ccgacaccca
     2641 cgtagtggaa gatactggct gcgaaggtag ccgcaaagcc aacagctgcg agcggtttcc
     2701 agatgccttt ccagaatttc acggtttcgc tgatttccgg gttctccggc aagccatgat
     2761 acagatttgg cttgtcagca tggtgcagca cgtacataac gtgtgtacca ccgacgcctg
     2821 ccggatcgta caggcccgca ttgtcgtaac cgcgggtttt cagctccgcc acgcgctcgc
     2881 tcgccagcgt tttcatcgac tctttcgtac cgaagtgaat cgcgcccgtt gggcaggtct
     2941 tcacgcaggc tggttcttgc ccaaccacca cgcggtcaac gcacagcgta catttgtaga
     3001 cgcggttgtc ttccgggttg aggcgcggaa tgtcgaacgg acagcccgca atgcaataac
     3061 cgcagccaat gcactgctcg gactggaagt cgacgatacc gttggcatac tgaataattg
     3121 caccttccgc cgggcacgct ttcaggcagc ctggatcgga acagtgcata cagccgtctt
     3181 tgcggatcag ccattccagt ttgtcgttct gctccacttc cgagaagcgc attaccgtcc
     3241 acgatttggc gcttaaatca ttggggttgt cgtacacccc aatgttattg ccgacggtat
     3301 cgcgaatgtc gttccactct gaacacgcca cctgacaggc tttacagccg atacaggtgg
     3361 tgacgtcgat gagtttcgcc acttcttcct ggaagtcccg cgcctgaggc gcgggggtca
     3421 gaccgttagt cgcggaacga cgaatgatat cttgagattg ataagccata tgtcgtctcc
     3481 gttacacctt ttccacattc acaaggaagg acttaaactc cggcgtctgc gtgttcgcat
     3541 caccaacgaa tggcgttaac gtattggcaa taaagccttt tttagctacg ccttcatagc
     3601 cccagtgaat cggaataccg atggtatcga tatctttgcc gttcgctttc agtgtacgaa
     3661 tacgtttggt caccaccgct ttggccttga tatagccacg gttggaggag actttcacgg
     3721 tatcgccctg ggcaatgcca agtttattcg ccagcgactc cccgatttcc acaaactgct
     3781 ctggttgcaa aatcgcgttc aacagcgcgt gtttggtcca gtagtggaaa tgctcggtca
     3841 gacgataggt ggttccgaca tacgggaact tatcggcttt acccaatgct tcggcgtcgt
     3901 ctttaaagat acgtgcagcc gggttcgaga taacgtttgg atgcagcggg ttagttccca
     3961 gcggcgtttc aaacggctcg tagtgttccg ggaacggacc ttccgccatc ttatcgaggg
     4021 caaacagacg ccccatgcct tcctgctgca tgataaacgg cccgacgccg ctacccggag
     4081 gcgctgcgct gtagtccgga atatcccagc cggtccactt agtgccgtcc catttcagca
     4141 actgacgctt cggatcccac gggttaccct gcggatctgc ggaggcgcgg ttatacagaa
     4201 tgcggcggtt aagcggccat gcccatgccc agcccagcgt gttaccgagg ccagacggat
     4261 cggcgttatc acgacgcgcc atctggttgc cttccggcgt ccagctaccg gcgaaaatcc
     4321 agcagccaca ggaggttgta ccgtcatcgc gcaattgggc gaacgagcta agttgttggc
     4381 ctttcttgac gataaccgca ccggttgccg gatcggtaat atcggccagc gccttaccgt
     4441 tgctctccat cgctacttct tccgacttcg gctcatgcgg aatggcgtag ttccaggtca
     4501 tgttcagcac ctggtccggg ttcgcgccac cctgttcggc atacatcttg cgcaagcgca
     4561 ggaagatacc ggagaggatc tcgccatcag tcagcgcaat ccccggggcg tccgcacctt
     4621 tccagtgcca ctgcaaccag cgacctgagt taacgattga accgttctct tctgcgaagc
     4681 aggtcgatgg cagacggaac acttcggtct ggatcttcga cgagtcaact tcgttcagct
     4741 caccgtggtt ctgccagaag ttagaggttt cagtgttcag tgggtcgatg gtgacgagga
     4801 acttcagttt cgacagacaa ccgatcactt tgtttttgtt cgggaatgag gcaacagggt
     4861 taaagccctg gcagatatag ccattgacct tgccctcttt catcatctcg aagtactgca
     4921 ggacgtcgta gcctttatcc cacttcggca accagtcaaa gccccagcta ttttccgccg
     4981 tcgctttatc accaaagaag gccttcatca tagagacgaa gaatttcggg tagttgcccc
     5041 agtagtttac ctggccttcc agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca
     5101 gatcggtctg cttctcgctt ggcagcgtca tgtaacctgg cagactctgc gacagtagcc
     5161 ccaggtccgt cagcccctga atattggagt gaccgcgcag ggcgttaacg ccgccgccgg
     5221 ccatccccat attgccgagc agcagctgga tcatcgccat cgtacgaatg ttttgcgcac
     5281 caacggagtg ttgcgtccag ccgagggcat acaggaacga ggcagtttta tcgtgagcac
     5341 tggtttctgc gatgtattcg cagactttca ggaacgcgtc ttttggcgta ccacagatgt
     5401 tttcaaccac atctggcgtg tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc
     5461 gcggatgttg cagcgtggta tcgcgtttgg cgaagccgtt ttcgtccagt tcgtaagtcc
     5521 aggaggattt atcgtacttg cgtttttccg cgtcatagcc ggtgaacagg ccatcttcaa
     5581 agccgtaatc ctcaagcacg atcaggctgg cgttggtata ggcttcggtg tattcgcggt
     5641 tgaatttttc attgttcagc aggtacagca atacgcctga caggaaagca atgtcagtac
     5701 cggaacgaat aggggcatag tagtcagcca ccgccgccgt acgcgtaaag cgaggatcga
     5761 tcacaatcag cttcgcgcca ttgtgaattt tggcttccat cgcccagcgg aacccgaccg
     5821 ggtgagcttc agcggcgtta ccgcccatca ccacgacgag gttggcgttc ttgatgtcga
     5881 cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc aagacttgct accgttggtc
     5941 cgtgtcagac acgcgcctgg ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt
     6001 gcgttaaata gccggtttcg ttactcgacg cggaagcaca cagcatcccg gtggagagcc
     6061 agcggttaac agtcacgcct tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt
     6121 tcatcagttt ggcgatgcga tcaaacgcct cttcccaact gatttgctgc catttatcag
     6181 aacctggcgc acggtattcc ggaaacttca gacggctttc ggagtggatg aaatccacca
     6241 ggccagcgcc tttcggacaa agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga
     6301 tatggaagat agatgctttg gcgttttttg ctccgtcacc gaggctgtac atcaacagcc
     6361 cacagcctac ggaacaatag gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat
     6421 actgccgggt ttccgcgagc gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc
     6481 ctgccatacc gccagcgcag atcttaaaga actgccttct gctgacctgc atggattgct
     6541 ccttgtttcg acattgtcac gtcccattta cattcgcttg ctgcgtgtgc agggagtggg
     6601 agttattttt ctttgcggaa ggggccgcaa aggtccagaa ttggctcaat ttccctccat
     6661 ccaggaaggg tttgtaacag aataccataa tgttggtgtg tgtgttctta tctggttaag
     6721 agaaagtgaa aaaaacacag caaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc
     6781 gtcaaattga gttatggcgg cgtgatgatt tacaacaccc acggttagat gaggtcgcgg
     6841 aagaagttcc cgttgcgctg gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc
     6901 ccaaagacct tgagtacttt gcgctcggtt tttcgctttc cgaggggata atagaaagtc
     6961 cgcgcgatat cttcggcatg gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg
     7021 agctttccag ccgccgcttt atggggttga aggagcgccg ccgggcgctg gcgggacgta
     7081 cgggctgtgg cgtatgcggc gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc
     7141 taccgttcac ccagacgttt gatctcaaca aactggatga tgcattacgt catctcaacg
     7201 attttcagcc agtggggcaa ctgactggtt gtactcacgc cgctgcctgg atgttgccat
     7261 ctggcgaact ggtcggcggg catgaagacg tgggtcgcca tgtggcgctg gacaaactgt
     7321 taggccgccg gtcacaagaa ggggaaagct ggcagcaagg tgcggtactg gtttccagtc
     7381 gtgccagtta tgaaatggtg caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg
     7441 tgtctgccgc gaccacgctt gctgtagaag tggctgagcg ttgtaatctg acgctggtgg
     7501 gtttttgtaa gccgggtcgg gcaacggttt atacccatcc gcagcgtttg agcaattaat
     7561 taaaataagt cctgcgatat a
//
LOCUS       DAZFRN010000206         7525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFRN010000206 DAZFRN010000000
VERSION     DAZFRN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.208"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            99..422
                     /gene="yecR"
                     /locus_tag="ACHKUB_004558"
     CDS             99..422
                     /gene="yecR"
                     /locus_tag="ACHKUB_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO6657478.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            593..1090
                     /gene="ftnA"
                     /locus_tag="ACHKUB_004559"
     CDS             593..1090
                     /gene="ftnA"
                     /locus_tag="ACHKUB_004559"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO6657479.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(1128..1367)
                     /gene="yecH"
                     /locus_tag="ACHKUB_004560"
     CDS             complement(1128..1367)
                     /gene="yecH"
                     /locus_tag="ACHKUB_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO6657480.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1558..2769
                     /gene="tyrP"
                     /locus_tag="ACHKUB_004561"
     CDS             1558..2769
                     /gene="tyrP"
                     /locus_tag="ACHKUB_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO6657481.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     SELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLAAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2831..3496)
                     /gene="yecA"
                     /locus_tag="ACHKUB_004562"
     CDS             complement(2831..3496)
                     /gene="yecA"
                     /locus_tag="ACHKUB_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO6657482.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3692..3778)
                     /locus_tag="ACHKUB_004563"
     tRNA            complement(3692..3778)
                     /locus_tag="ACHKUB_004563"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3742..3744),aa:Leu,seq:taa)
     gene            complement(3791..3864)
                     /locus_tag="ACHKUB_004564"
     tRNA            complement(3791..3864)
                     /locus_tag="ACHKUB_004564"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3830..3832),aa:Cys,seq:gca)
     gene            complement(3919..3994)
                     /locus_tag="ACHKUB_004565"
     tRNA            complement(3919..3994)
                     /locus_tag="ACHKUB_004565"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3959..3961),aa:Gly,seq:gcc)
     gene            complement(4146..4694)
                     /gene="pgsA"
                     /locus_tag="ACHKUB_004566"
     CDS             complement(4146..4694)
                     /gene="pgsA"
                     /locus_tag="ACHKUB_004566"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO6657483.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4751..6583)
                     /gene="uvrC"
                     /locus_tag="ACHKUB_004567"
     CDS             complement(4751..6583)
                     /gene="uvrC"
                     /locus_tag="ACHKUB_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO6657484.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6580..7236)
                     /gene="uvrY"
                     /locus_tag="ACHKUB_004568"
     CDS             complement(6580..7236)
                     /gene="uvrY"
                     /locus_tag="ACHKUB_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO6657485.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7378..7449
                     /locus_tag="ACHKUB_004569"
                     /pseudo
     CDS             7378..7449
                     /locus_tag="ACHKUB_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         1813 a         1935 c         1780 g         1997 t
ORIGIN      
        1 gatggcaaga aatagcgcct gccaggcgtc ttttccggcc attgtcgcag cgctgtaacg
       61 cgtaaaatag tgctttctct tactcttctg gctggaccat gagacttctg attctgactc
      121 tttcattaat aacgctggcg gggtgtacgg ttactcgcca ggcccatgtg agcgaagttg
      181 acgcggcaac aggtattgta cggttggttt atgaccaggc ttttttgcag catgcccata
      241 ccgatcgtta tgtcagtcgc gggatcgccg atcgcgcatg ccagcaggaa ggctataccc
      301 acgcggtccc ctttggtcag ccagtaggca actgtagcct ttttgcgggt tccttatgtt
      361 tgaatacgga attcactttg tcatatcagt gccatcattc cgccttccct gtctttctct
      421 aaatatttta atcagcgagg ggatcttcgc tgattaaaga aatagcggta atgcgtttta
      481 ttcccattcg tatttttaat aattgaagtt tatattttac cttttgcaaa taataaaata
      541 acaaattata gtgacgccac agcaacaaat ataacctttg tggagcacta tcatgctgaa
      601 accagaaatg attgaaaaac ttaatgagca gatgaacctg gaactgtact cttcactgct
      661 ttatcagcaa atgagcgcct ggtgcagcta tcataccttc gaaggtgctg ccgcgttcct
      721 gcgccgtcac gcccaggaag agatgacgca tatgcagcgt ctgtttgatt acctgactga
      781 taccggcaat ttaccgcgta ttaataccgt tgaatctccg tttgctgaat attcctcact
      841 tgatgaatta ttccaggaaa cctataaaca cgaacaatta atcacccaga aaattaacga
      901 actggctcat gctgcaatga ccaatcagga ctacccaaca tttaatttcc tgcaatggta
      961 tgtttctgag cagcatgaag aagagaaact gttcaaatcg attattgata aattaagcct
     1021 ggcaggcaaa agcggcgaag gtctgtattt tatcgacaaa gaactctcta ccctcgacac
     1081 acaaaactaa tgctctccgg cggcaggttt actgccgccg tttcaaatca gtgacggcaa
     1141 atcttactct gatcggtcga aaaaccgtct ttcgaaggta taaatttgcc ttttgctgcc
     1201 agaaacgcta ccagctctcc cgctgtcatc ccttctgccg agcaggtgtg aaaacgtgcc
     1261 tgttcaccaa aacgcgcttt aatcgcagct tccagactgg catgcgtata ttgctcgcct
     1321 gattcaatca tcatatttaa cacttcatga ccgtgaatag agtccatcgt ccctcctcaa
     1381 aaaaagccta gcgtagcgat tgccgcttat gaagactttg cgccagcgca ggactgaatg
     1441 ctttttattg tacatttata tttacaccat atgtaacgtc ggtttgacga agcagccgtt
     1501 atgccttaac ctgcgccgca gatatcactc ataaagatcg tcaggacaga agaaagcgtg
     1561 aaaaacagaa ccctgggaag tgtttttatc gtggcgggaa ccacaattgg cgcaggcatg
     1621 ctggcaatgc cgctggctgc ggccggtgtt ggttttagcg ttacgttaat cttgttgatt
     1681 gggctttggg cgttgatgtg ctacacggcg ctattactgc tggaggtgta ccagcatgtt
     1741 ccggcagata ccggtctggg cacgctggca aaacgctatc tgggacgcta cggtcaatgg
     1801 ctgacgggct tcagtatgat gttcttaatg tatgctctga ctgcggcata catcagcggt
     1861 gccagtgaat tgttggcctc cagcatcagc gactggacag gtatttctat gtcggcaacc
     1921 gctggcgtgc tgttgttcac ttttgttgcc ggtggcgtgg tttgtgttgg aacttcactg
     1981 gtcgatttat ttaaccgttt tctgttcagc gccaaaatta tttttctggt ggtaatgctg
     2041 gtactactgc tgccgcatat tcacaaagtg aatcttttaa ccctgccgtt gcaacagggg
     2101 ctggctctgt ctgcaatccc ggtgattttt acgtcgtttg gttttcacgg tagcgtgccg
     2161 agtattgtca gctatatgga tggcaacatt cgtaagctac gctgggtgtt tataatcggt
     2221 agtgcgatcc ccctggcggc atatattttc tggcaggtgg cgacgcttgg cagcattgat
     2281 tcaacaacct ttatgggatt gctggctaat catgctggat taaacgggct gttacaggcg
     2341 ttacgcgaaa tggtggcctc tccgcatgtt gagctggcag tgcatttatt tgctgattta
     2401 gccctcgcca cgtcatttct cggcgttgcg ttaggcttat ttgattatct ggctgattta
     2461 tttcagcgtt caaataccgt tggtggacgg ttgcaaactg gtgcaattac gtttctgccg
     2521 ccgttggcgt ttgcactgtt ttatccacga ggatttgtga tggcgctggg ttacgccggt
     2581 gtggcgctgg cggtactggc attgattatc ccttcgctgt tgacctggca aagcagaaag
     2641 cacaatcctc aggcgggtta ccgggtcaaa ggtggtcgtc cggcgctggt ggtggtgttt
     2701 ctctgtggta ttgctgtgat tggcgtgcaa tttttgattg cggcagggtt gttaccagaa
     2761 gtggggtgat cagatagcct caaattcctt attgggtgcc agaattaacg ctgacaccca
     2821 atttggcctc ttaatgcagg cagcactgct taaatttctt accactaccg cacgggcaag
     2881 gatcgttacg ccccggtttc tcttctgctt tgatcggttg ctgaacagct ttttcctgcg
     2941 gatgcgccat ccagtacgca tgtagatcaa gcgccgccag tcgaatggca tctacgctct
     3001 cttcaaacgc ttctggcgac atcttttcta cccgctcgaa gttttcctca gtaccgtgca
     3061 gcgcaatcgc ctccagcgct ggttttaacg aatcgggcaa cgttgaccag tcagaaagtg
     3121 ccacgccccg catatagcca aagcaccact cctcaacaat cgtcagctcg ctgccatcaa
     3181 cttctcgcaa gccgaataac ggctcaaact gctccgggaa ttcgttcaga cgctctgcgg
     3241 tatcggccat atgttgaaaa gccagattca taaagcgcgt catctctttc tctgacgccc
     3301 agcgcggcac atagtcagcc ccaccccaca cggcaaccag ccactgttcc ggttcaatct
     3361 cttgcggaga actcaacacc gccgtcaata aaccgtccag ctccgccaca tcaaggatgg
     3421 cgtggtcagt gttgtatttg gtcagaatat cgtccagcca ttccaactca ctttcgttta
     3481 acggtcccgt tttcatacgc ttttccttgt ggatctcaac tcgccagcac ctatcttaca
     3541 tgccggtccg tatcagagat actttttgag tggctttgct ggtgattaaa aattaaggag
     3601 ggtgtaacga caagttgcag gcacaaaaaa accacccgaa ggtggtttca cgacactgct
     3661 tattgctttg attttattct tatctttccc atggtacccg gagcgggact tgaacccgca
     3721 cagcgcgaac gccgagggat tttaaatccc ttgtgtctac cgattccacc atccgggctc
     3781 gggaagaaag tggaggcgcg ttccggagtc gaaccggact agacggattt gcaatccgct
     3841 acataaccgc tttgttaacg cgccaaattc ttcaggcctt tcagccagac atccgcttga
     3901 cgccgatgtc ttttaaactg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg
     3961 caaggtcgtg ctctaccaac tgagctattc ccgcattcat caagcaatca gttaatcact
     4021 tgattttatt atcgtctggc aatcagtgcc gccgttcgat gcgttgcatt ctacttacct
     4081 ggcgcgatga gtcaacgata tttttcacca cttttgatcg tttgctgaaa attacgccga
     4141 aacgatcact gatcaagcaa atctgcacgc gcagcgctca aatattgcaa cattgaccac
     4201 agagtcagta ccgcagccac aaagaaaagt gcaataccgg cgtactcaac ccaaatgttc
     4261 ggacgccaca gcagccatgc caacgccacc atctgggcag tggttttcac tttcccaatc
     4321 caggagacgg ccacgctact gcgtttaccc aactccgcca tccattcgcg tagcgcagaa
     4381 ataataattt cacgggcgat catcgttgcc gccggtaatg tcacccacca gctgtgataa
     4441 tgctcggtta ccagcaccat ggcgatagcc acgagaactt tatctgccac agggtcaagg
     4501 aaagcaccaa accgggtact ctggttccag cggcgtgcca gaaaaccatc gaaccagtca
     4561 gtcaccgccg cgacgcagaa aatgagcgcg gcggcaaacg gcgaccaggt gacaggcaga
     4621 taaaagacca atacaaagaa tgggataagg atgacacgga acagtgtaag caacgtaggg
     4681 atattaaatt gcataatgac gggtaactat ctgttgtcag taagattacc cctatgttgc
     4741 tacagagaca tcaatgtttc aacgaccaga agatcttttc tgccagacct tgcgaaatac
     4801 ccggcacttt tgcaatttcc tcgacgctgg cgttacgtaa accttgcaaa ccgcccatat
     4861 atttcaacaa catttgccga cgttttggcc cgacgccttc aatggtttcc agggaactgg
     4921 tatttttgac cttcgcccgt tttttacggt gcccgccaat cgcgtgatca tgtgattcat
     4981 cgcgaatatg ctggataaca tgcagcgcgg gtgaatctgg cggcaaacta aatccctcac
     5041 cttccggctc aaagaacagc gtttccagtc cagccttacg atctgctcct ttggcaacgc
     5101 caagtagcag cggatgattt ttatcccatg agacatccag ttcggcgaag acatttttcg
     5161 cctgcgcaag ctggcctttg ccgccgtcga taaggatcac atccgggatc ttactgtcgt
     5221 caatggcttt accataacgc cgacgcagca cctgattcat cgccgcataa tcatcgcccg
     5281 gcgtgatgcc tgtaatgtta tagcgccgat actccgcacg cagcgggccg ttagcatcaa
     5341 acaccacaca ggaagcgacg gtttgttcgc ccatggtatg gctgatgtca aagcactcca
     5401 tccgcttcac ttccggcaat ttcaacacgc tggcaagcgc ggtcagtcgc tggtgaacgg
     5461 tagattgctg cgaaagtttg ctggttaagg ccgtcgccgc attggtgcgc gcgagtttca
     5521 gataacgcgc cctatcgccg cgaggtttgg tttgaacatt aatcttgcgt cccgccagtt
     5581 ctgaaaggga atcggcgagc agcgttttat cgctaagatt aaaatcgagc aggatctcac
     5641 ccggtaaggt gcgcatctgg ctgccttgta aatagaactg gcctacgaag gtttctacca
     5701 cctcgctcag ttccgtaccg ccaggcactt tcgggaaata gctgcggctg ccgagcactt
     5761 tgccctgacg aatgaacaat acgtggacac aagccatgcc cgcatcgaac gccacaccaa
     5821 taacgtcgag gtcgtcgccg gtattggaaa cgaattgttt ttcggtgacg cgtcgcaccg
     5881 cctgaatttg gtcgcgaata cgtgcagctt cttcaaactc cagattctgg ctggcagttt
     5941 ccatacgact aatgagttgc gtaagcacct gatcatcttt gccagacaaa aacaggcgca
     6001 catactcgac ctgctgagcg tattcttctt cactcaccag tccttcaacg cacggtccca
     6061 gacagcgccc tatctggtat tgcagacacg gacgcgagcg attgcgataa acactatttt
     6121 cgcactggcg aatggggaaa atcttttgca gtagcgccag tgtttcacgt acggcatagc
     6181 cattcgggaa cgggccgaaa tattcacctt tggcatgctt cgcaccacga tgcatcgcca
     6241 gacgcgggtg ggtatcacca ctcaggaaga taaaaggata tgatttatca tcgcgtagca
     6301 aaacgttgta acgcggctga tagagtttga tgtagttgtg ttccagcaac agcgcttcgg
     6361 tttctgtgtg agtaaccgtt acatcaattt gctggatctg ggcgaccagc gcttcggttt
     6421 tgcgcgaagc gaggttgcta cggaaatagc tggaaagccg ttttttcagg tctttcgctt
     6481 tgccgacata gataaccgta ccaccagcat cgtacatgcg ataaacgcct ggctggctgg
     6541 ttacggtttt taaaaacgct tttgcgtcaa actgatcact cactgacttg ataatgtctc
     6601 cgcattacac agaccatggc gaattgccag gtgagtcagc tcaacatcgc catgaatgtt
     6661 tagtttactg aacatacgat agcggtagct gttcaccgtt ttcggactga gattgagctg
     6721 ttctgagatc tcattgacct tctggccctt ggtgatcatc agcataatct gcaattcacg
     6781 ttcagacaaa ctggcaaatg ggctttctgt tttttctggt tcgatctggc ttaacgccat
     6841 ttgttgagcg atgtcagaag caatgtaacg ctgccctgaa tagacagaac gaatcgcact
     6901 cacaacttcc tgcggagccg cgcctttgct gaggtagccc gcagcaccgg cctgcatgac
     6961 tttcgctggt aaagggtttt ctgtatggac ggtaagcatg atgattttga catcagctgt
     7021 ggaacgcgcg attttacgcg tcgcctcaag accgccaatg cccggcatac tcatgtccat
     7081 tagcaccacg tcaacggcat ttgtccggca ccacttaacg gcgtcttcac cgcacgatgc
     7141 ctcaccgacg acttttatac cctttatatc ttccagaatg cgtcgtatcc ctgcgcgcac
     7201 cagttcgtgg tcatcaacaa gtagaacgtt gatcaaagga atatctccag aaatagggat
     7261 aacgctactg atagttagtc attcgtatat tagcggtttt tattgcaact ttgaaacgtt
     7321 aaaaatggtg ggtattcgat ttttctctcg tttttgtcaa ttcgattgtc cacagttatg
     7381 gtcacgcccg tcaccaacgg cttaccggat cgtttcacag taagccctac gatgaaatgt
     7441 gacaaaaatt gactttattc agcaaaaatg aaaatcagcc cgctgaatat gtaacattaa
     7501 ttaacccgaa caatacgggc aaaaa
//
LOCUS       DAZFRN010000207         7523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFRN010000207 DAZFRN010000000
VERSION     DAZFRN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.209"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            2..850
                     /gene="gatY"
                     /locus_tag="ACHKUB_004570"
     CDS             2..850
                     /gene="gatY"
                     /locus_tag="ACHKUB_004570"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO6657486.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALEFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
     gene            879..2141
                     /gene="gatZ"
                     /locus_tag="ACHKUB_004571"
     CDS             879..2141
                     /gene="gatZ"
                     /locus_tag="ACHKUB_004571"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO6657487.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIEHTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2151..2603
                     /gene="gatA"
                     /locus_tag="ACHKUB_004572"
     CDS             2151..2603
                     /gene="gatA"
                     /locus_tag="ACHKUB_004572"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO6657488.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2634..2918
                     /gene="gatB"
                     /locus_tag="ACHKUB_004573"
     CDS             2634..2918
                     /gene="gatB"
                     /locus_tag="ACHKUB_004573"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO6657489.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            2922..4277
                     /locus_tag="ACHKUB_004574"
     CDS             2922..4277
                     /locus_tag="ACHKUB_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO6657490.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4325..5365
                     /gene="gatD"
                     /locus_tag="ACHKUB_004575"
     CDS             4325..5365
                     /gene="gatD"
                     /locus_tag="ACHKUB_004575"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6657491.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPDDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLVLVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            5471..6244
                     /locus_tag="ACHKUB_004576"
     CDS             5471..6244
                     /locus_tag="ACHKUB_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6657492.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHKUB_004577"
     CDS             complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHKUB_004577"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO6657493.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1891 a         1694 c         1955 g         1983 t
ORIGIN      
        1 cgtggtatcg acaaagcaaa tgctgaacaa cgcacagcgc ggcggttatg cggttccggc
       61 attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg ccaacctgca
      121 tgcgccggtc atcatcgccg gaacgcctgg cacatttact catgctggta cagaaaatct
      181 gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa ttcatctcga
      241 ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc gctcagtcat
      301 gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag aggtggtgga
      361 tttttgccat cgctttgatg tcagcgtcga agcggagctg gggcaacttg gcggccagga
      421 agatgatgtg caagtcaatg aagccgatgc tttttatacc aaccccgctc aggcgcgtga
      481 atttgccgag gcaaccggaa ttgattccct ggcggtcgcc atcggcacgg ctcatgggat
      541 gtatgccagc gcaccggcgc ttgaattttc tcgactggag aacattcgcc agtgggtgaa
      601 cttaccgctg gtgctgcatg gcgcgtcagg gttatcgact aaggatattc agcaaaccat
      661 caaactgggg atatgcaaaa tcaacgttgc aacggagctg aaaaatgcct tctcgcaggc
      721 gttaaaaaat tacctgaccg agcaccctga agcgaccgat ccccgggatt atttgcagtc
      781 ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg gctgcgaggg
      841 cagggcataa cgcacctgcc atttaacaag gaaaaaacat gaaaacgtta attgcccggc
      901 ataaagctgg tgaacatatc ggcatatgtt cagtctgttc tgcccatccg ttggttatcg
      961 aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa
     1021 accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt cgcgaatttg
     1081 tttttacgat tgccgataaa gttgggtttg cacgcgaacg cattattctc ggcggcgatc
     1141 atctggggcc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg
     1201 agctggtaaa ggaatatgtt cgtgccggct tcagtaaaat tcatcttgat gcgtcaatgt
     1261 cctgcgcggg ggatcccata ccgttagcac cagaaacggt tgcggaacga gctgctgtgc
     1321 tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg agctatgtca
     1381 ttggcaccga agttccggtt ccgggcggtg aggccagcgc cattcagtca gtacacatca
     1441 cccatgttga agatgccgcc aatactttac gtacgcatca aaaggccttt attgcccgtg
     1501 ggctgacaga ggcgttaaca cgtgtgattg ccatcgtggt gcagccgggt gtggaatttg
     1561 atcacagcaa tattatccat tatcagccgc aggaagcgca ggcgctggcg caatggatag
     1621 aacacacccg aatggtttat gaagcacatt ctaccgatta ccagacccgg acggcttatt
     1681 gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg tcccgcatta acctttgctt
     1741 tacgcgaggc gatatttgca ctggcacaaa ttgagcagga acttatcgcc cccgaaaatc
     1801 gcagcggttg cctggcggta attgaagaag tgatgctcga cgaaccgcaa tactggaaaa
     1861 aatattatcg tacgggtttt aacgattcat tactggatat tcgttacagc ctgtcggatc
     1921 gtattcgtta ttactggccg catagtcgga ttaaaaatag cgtcgaaacg atgatggtga
     1981 atctgcaagg cgtggacatc ccactgggca tgattagtca gtatcttccc aaacaatttg
     2041 aacgcattca gtccggggaa ttatcagcaa taccgcatca gctgattatg gataaaattt
     2101 atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat atgactaacc
     2161 tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc catatcggta
     2221 atgagatgct cgccaaaggt gtggttcatg atacctggcc acaggcatta attgccagag
     2281 aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgcaata ccgcattgtg
     2341 aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat aaagttcatt
     2401 ttcagcaagc ggatgatgat aacgacgtgg aggtatcgtt ggttattgcg ttgattgtgg
     2461 aaaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta caacagcccg
     2521 atatcgtcga gacactaatc actcttcctg aaacccagtt aaaggaatac ttcacaaagt
     2581 atgttttaga ttcagacgaa taaatctctc tgtaacaata aaaaggacta tttatgaaac
     2641 gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg gcggaagaaa
     2701 ttaaagagtt gtgtcagaat cataatattc ctgttgaatt aatccagtgt cgggttaatg
     2761 aaatagaaac ctatatggat ggtgtgcatt tgatatgcac cactgccaaa gtggatcgta
     2821 gttttggcga tattccgtta gttcacggca tgccttttat ttctggtgtc ggtatcgaag
     2881 cattacaaaa taaaattctg actatcttac aggggtgacc tatgttttca gaagtcatgc
     2941 gttatattct cgacctcggc cctacggtga tgcttccgat tgtcatcatt attttttcta
     3001 aaatattagg catgaaggca ggcgattgct ttaaagcggg tctgcatatc gggattggct
     3061 ttgttggcat tggccttgtg attggcttaa tgctggattc cattggtccg gcggcgaaag
     3121 cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg ccgggctctt
     3181 caccaatgac ctgggcttcg caaattgcgc tggtggcgat tccgattgcg attctggtta
     3241 acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc tggaatatct
     3301 ggcatatgac cttcaccggc gcgttgctgc atctggcaac cggttcatgg atgataggga
     3361 tggcaggtgt cgtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
     3421 atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg tcggcgtata
     3481 tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgaa
     3541 ttaaatttag cgccgacgat attcagcgca aatttggtcc atttggcgag cctgtcaccg
     3601 tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc aaaggtgtat
     3661 tgcagctggc ggtaaaaacg gcggcagtga tgctgctaat gccacgggtg attaaaccca
     3721 tcatggatgg tttaacgccc atcgctaagc aggctcgtag tcgtttacag gcgaagttcg
     3781 gcggtcagga gttcctgatt ggccttgatc cggcgttgct gctgggacat acggcggtgg
     3841 tatcggcaag cctgattttt atcccactca ccattttaat tgctgtttgt gtgccgggta
     3901 atcaggtgct gccgtttggc gatcttgcca ccatcggctt cttcgtggcg atggcggtcg
     3961 ccgtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatt atgagcatca
     4021 ccctgtggat cgcgacgcaa actattggtt tgcacaccca actggcggct aatgctgggg
     4081 cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg cggttctccc attacctggt
     4141 tactgattca ggttttctcc ccgcaaaata ttcccggttt cattattatc ggtgcaattt
     4201 atctgaccgg tattttcatg acctggcgta gagcgcgtgg ctttattaaa caagagaaag
     4261 tcgttctcgc agaataattt ttacctgagg gggaggttat cccccttaac aatcaggagt
     4321 ttttatgaaa tcagtggtga atgatactga tggtatcgtg cgcgttgcag aaagcgtcat
     4381 tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg gcttatgtgg
     4441 ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa cgttaggcca
     4501 tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac accctgacga
     4561 tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtc tgaaagggtt
     4621 ttattcccag tgcgcaaaat atgattttat tggctcgcgg cgtgatggtg gatttgctga
     4681 atatattgtc gttaagcgaa aaaatgtctt tgctctaccc acggatatgc ctattgagga
     4741 tggggctttt attgagccga ttaccgttgg tctgcatgct tttcatttag cgcaaggttg
     4801 tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg ctattcagtg
     4861 cgctgtcgcg ctgggagcaa agagtgtgac ggctatcgac attagctcag aaaaactggc
     4921 actggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga gcgcgccgca
     4981 aatgcagagc gttttacgcg aactgcgttt taatcagctt atcctcgaga cggctggcgt
     5041 accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac tggtgctggt
     5101 gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa tattgcgtaa
     5161 agagctgacg gttatcggca gttggatgaa ctattccagc ccttggccgg ggcaggagtg
     5221 ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat taatcgctca
     5281 ccgtggaagc tttgaaagct tcacccaggc ggtgcgtgac atcgctcgta atgccatgcc
     5341 gggcaaagtg ttgctcattc catgaaaccg cgggccagcg tgatgctggc ccggtattgt
     5401 gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa ttgaatatgg
     5461 gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag tgaatgaaca
     5521 gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag cgacaatccg
     5581 tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg gcggtgcggc
     5641 gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta aagagcgatt
     5701 tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa tgatccacga
     5761 agggatgacc gttattctcg acagcggaag tacgacgatg cttatcgccg aaggattaat
     5821 gaccgccaaa aatatcacgg tgattaccaa cagtctcccg gcagcgtttg ccctttccga
     5881 aaataaagac attactctgg tcgtctgcgg tggcacggtc cgccataaaa cgcgctcgat
     5941 gcatggttcc attgccgagc gttcattgca ggatattaat gccgatttaa tgtttgtcgg
     6001 tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt attcgattag
     6061 cggggcgatg gtcaccgccg ctaataaagt gattgccgtt ctcgattcat cgaaatttaa
     6121 ccgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattatta ttaccgatga
     6181 tgctgtgtct gaggtggata aactggcatt gcagaagaca cgggtaaaat taattacggt
     6241 gtagtattgt tctgtatgcc ggataaggta tttacactgc atccggcatt tcggattctc
     6301 cctggttcaa gagagaatag tttgattaac gcaataatgg acaatctggt ggtaatcgac
     6361 aacgcaacgc cgccggaagt atttcaatat gaaagttttg tccgctcaac ggttcgccat
     6421 caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa gcgccttcga
     6481 taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga agaatttcat
     6541 cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgcgttc ggacacaatt
     6601 gctgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg tcaccttgcc
     6661 agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc atgcgcatta
     6721 agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc ggcgtttctg
     6781 tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt tgtttgttga
     6841 cctgcgccat atctatcgca atggcgttac cggcaattgc cagtttcagc gccttatcca
     6901 gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat ggcaaaattc
     6961 ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact tcattaatgg
     7021 tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc tcctctacat
     7081 atcgtgcggc atcgcctttc tcccaggtga cccgcacatg gatcgtcatt ccttcctcac
     7141 gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttgcca ttaagaatca
     7201 gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt ctgtagatag
     7261 tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg ataaaaagtc
     7321 agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg acagcgaata
     7381 ctaacggctg ccaccgataa atttcaaaaa agagcatatg cctaatattc aaccaaacag
     7441 tggcatctta aatatactat tccaaatata ttaaagcccc catggagtta ccctgaaggg
     7501 cctcaatgtc cgtaattcct act
//
LOCUS       DAZFRN010000208         7435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFRN010000208 DAZFRN010000000
VERSION     DAZFRN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.210"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..1640)
                     /locus_tag="ACHKUB_004578"
     CDS             complement(<1..1640)
                     /locus_tag="ACHKUB_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO6657494.1"
                     /translation="MAGNFADLTAVLTLDSTRFSEEAARVKKELGETSDLADLMAGRV
                     SQSFKKQASAVEQGLSRQALAAQKAGMSVGQYKAAMRMLPMQFTDVATQLAGGQNPWL
                     ILLQQGGQVKDSFGGLIPMFRGLAGAVSLPAVGITSLVAATGALAYAWYQGDSTLSAF
                     NKNLVLSGNQSGLTADRMLALSRAGQAAGLTFNQAGESLAALVSAGVRGGEQFDAINQ
                     SVARFASASGVEVDRVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDE
                     AGALQAANDIATKGFDDQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSAD
                     MLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSD
                     MEKAGELAARDRAEREASQLKYTGEAQKAYERLLSPLDKYTARQKELNQALKDGKILQ
                     ADYNTLMASAKKDYESTQKKPKSSGVRVSAGERQEDRAHAALLALETELRTLEKHSGA
                     NEKISQQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLA"
     gene            complement(1630..2034)
                     /locus_tag="ACHKUB_004579"
     CDS             complement(1630..2034)
                     /locus_tag="ACHKUB_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6657495.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRRDDEQTEMPDEDDMLMRKAAGLSGGVRFGADGKEI
                     VTVSDDMRSSTEDEAMLMMVSEGIPGGVRYGG"
     gene            complement(2061..2492)
                     /locus_tag="ACHKUB_004580"
     CDS             complement(2061..2492)
                     /locus_tag="ACHKUB_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6657496.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(2512..3261)
                     /locus_tag="ACHKUB_004581"
     CDS             complement(2512..3261)
                     /locus_tag="ACHKUB_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6657497.1"
                     /translation="MATPNPLEPVKGSGTTLWVYTGTGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SESRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDQGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            complement(3269..3667)
                     /gene="gpU"
                     /locus_tag="ACHKUB_004582"
     CDS             complement(3269..3667)
                     /gene="gpU"
                     /locus_tag="ACHKUB_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO6657498.1"
                     /translation="MRHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            complement(3680..4303)
                     /locus_tag="ACHKUB_004583"
     CDS             complement(3680..4303)
                     /locus_tag="ACHKUB_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6657499.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARELAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVLLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(4306..4587)
                     /locus_tag="ACHKUB_004584"
     CDS             complement(4306..4587)
                     /locus_tag="ACHKUB_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO6657500.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(4580..4906)
                     /locus_tag="ACHKUB_004585"
     CDS             complement(4580..4906)
                     /locus_tag="ACHKUB_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO6657501.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSAAV
                     AVKINV"
     gene            complement(4994..6973)
                     /locus_tag="ACHKUB_004586"
     CDS             complement(4994..6973)
                     /locus_tag="ACHKUB_004586"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO6657502.1"
                     /translation="MKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFVSELNALGD
                     ITHINLHINSPGGDVFEGIAIFNSLKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKSLFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(6963..>7435)
                     /locus_tag="ACHKUB_004587"
     CDS             complement(6963..>7435)
                     /locus_tag="ACHKUB_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6657503.1"
                     /translation="ARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNM
                     VVAAGLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAG
                     GRNPDEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
BASE COUNT         1553 a         2344 c         1802 g         1736 t
ORIGIN      
        1 gccagcaggg atttttcctg cccggataac tgtcgtttcg tggcggcctc tttcaggacc
       61 gcatactgac tttccgcttt ccataaatca cgacgctgct ggctgatttt ctcattcgca
      121 ccgctgtgct tctccagcgt ccggagctca gtttcaagcg ccagcagggc tgcatgcgcc
      181 cggtcttcct gacgctcccc ggctgacact ctgacacctg acgacttcgg ttttttctgc
      241 gttgattcat aatccttttt cgccgacgcc atcagcgtgt tgtaatctgc ctgcaggatt
      301 ttcccgtctt tcagggcctg attcagctct ttctgccggg cggtatattt atccagcggc
      361 gacaacaggc gttcatacgc tttctgcgcc tctccggtat acttcagctg tgacgcctca
      421 cgctcagccc tgtcccttgc cgccagttca ccggcttttt ccatatccga ctgcagcgtt
      481 gccgccgcca gccccagacg ggcattttcc cggtcatccc atgcaccctg aaggttggcc
      541 cggaaagagg aggttttacc gcggcgctgg ctccggctct ggtaccactg ccatttttta
      601 tccgcctcat caaatgcctt ctgcgcactg gcgagcatat ccgctgagga ttcaggacga
      661 ccgatatcca gaatggcatc ccacatcgat ttgaacgcct tccctgtttt atctgcccag
      721 gtttccagcg tccccatgtt ctctttcagg cggcgggtct ggtcatcaaa gcctttcgtg
      781 gcaatatcgt tcgccgcctg taaggcaccg gcctcgtctc cggaacgctg cagctgtgca
      841 acatacgcaa tctgctctgc cgtcacgtta cggaactggc gcgccatcgc catcagtccc
      901 gacgtcgggt cggtggtcag ctttccgaaa gcctctgcaa ccctgtccac ttccacaccg
      961 gaggcagacg caaaacgcgc cacactctgg ttaatggcat caaactgctc accaccacgc
     1021 acaccggcac tgaccagggc tgccagtgac tctcccgcct ggttaaacgt cagccctgct
     1081 gcctgtccgg ctctggagag cgccagcatg cgatcggcag tcagtccgga ctgattaccg
     1141 gaaagaacca ggtttttatt aaacgctgaa agcgtggaat ccccctggta ccaggcatac
     1201 gccagcgcac ctgtcgcggc gaccagtgat gtgatcccga cggcaggtag cgagacggct
     1261 cccgccagtc cccggaacat cgggataaga ccaccgaacg agtctttcac ctgaccgccc
     1321 tgttgcagca ggatgagcca gggattctga ccaccggcaa gctgcgtggc aacgtcggtg
     1381 aactgcatgg gtagcatacg catggcggct ttatactgcc cgacggacat cccggctttt
     1441 tgtgcagcca gcgcctggcg gctcagcccc tgctcaacgg cagaggcctg tttcttaaaa
     1501 gactggctga cacgcccggc catcaaatcc gcaaggtcac tggtttcacc cagttctttc
     1561 tttacccgtg cagcctcttc agaaaaacgg gttgaatcca gtgtaagaac agctgtcaga
     1621 tcggcaaaat tacccgccat agcgtacacc tcctggaatt ccctcagaca ccatcatcag
     1681 catggcttca tcctctgtac tgctccgcat gtcatcactg accgtaacga tttccttccc
     1741 gtcagcccca aagcggacac caccagaaag tcctgccgct ttccgcatca gcatatcgtc
     1801 ctcatccggc atctccgtct gctcatcatc acgtcggggt gccagcagac tgaaatcaga
     1861 gggatgcata tccggatcgc aaaaaaacag gctgagtaca gcgtacgtca gcccggaaaa
     1921 atgcatatcc agctgggtat catgaaaata atgcgtgcgg taaaaacgtc gccagtcggc
     1981 atattcggtg gatgtcatcc cggcaagcat ggcgcgccag tcgggtctcc ccatctcacg
     2041 cgccagtctg agggcaaagt tcagctcgcc gtcgaagact ttcccgcaga aaaatcatca
     2101 tcagtcagcg cgttattttt cgccacttca gtaatatcag tatccggacg aacagcttcg
     2161 atcataccgg acaggcacaa caccacgtct tccgcccggg caatggcatc ggcaggccag
     2221 gtggtgagca cttcctgctc tatcttcatc acggcctcat tcattgacgg tgactgcgtt
     2281 ttctgtggat ggttatgcca cagggacatc gccaccagaa acgcgccggt tctgacaaga
     2341 tcttccacac tcacctgcag gttgccgctg gcttcagcct cttctgcccg ccgtttcagg
     2401 agggcaagat gctcaatacg ctgcagcgca gacagctcag aaagcgtgac agacaccccg
     2461 ttatattcaa attgttctgt tttcagaaac atgctttatc actccctcag cttaacccgc
     2521 tgcaccatcc gtgacggtaa tttccgccac tgccgcaaac tcaccattgc cggtaaccac
     2581 agggatctgc gctttacctg ccgcaacacc tttcacggtg atcgtgttcc ctttcacggt
     2641 aatggtggcg aaattctgat tcgccgacgt ggcacggaag gttttatccg tcgccccttc
     2701 cggctgaaca gccacggtca gggtgatatt ctgacctttt gccacattgc ccgttggtgg
     2761 cgtcacggta ataccggtga ccggtgtgat gtccccctga tcttccgcca gcgacggacg
     2821 accgatattg gtgattttta ccgtacgggt gatcacctct ttggcggtca ccgctttacc
     2881 aatggcgctc acccagccac ggaacacatc caccgtaccg ttcgggaagc gaattttgta
     2941 ggcccggctc tcactgctgt caaaccaggc aatcaaatcg cgctgccctt tctcacccgg
     3001 cttccaggcc agcgtaaaac tggtgtcacc ggcagatttc tgcccctgcc cggtggatac
     3061 ccagtcagca tcctcatcat ccagatagtt atcatcgtag gattctgccg tcatctcgcc
     3121 gggggtcaga tccttcacct tcgccagtcg ctgccagtca tcgtctgaca acgggtttgc
     3181 ataagcatca ccagtgccgg tgtacaccca cagtgtggta ccggaacctt tcaccggctc
     3241 aagaggattt ggtgttgcca tatcgtcctc acatctcgta ggtaattttc cacaggagat
     3301 ctgccgatcc ccacatcata aactcatcat cccggcggta gtcatacccc tgaagattca
     3361 ttttcagcag taatgcactg agcccgggaa ctgcctccag cgcaggaaga attttttctt
     3421 ccatccacat atccagtgcc gagtccggtt cttttgccct gagaaaaacc tcaatatgca
     3481 gtgtcgcctc ccaggttccc tcatcaacga actcgtcagc agcagacgca tctgtcaggt
     3541 aaacagcaac agcaggcagt tcctgttcat caataaaaac cgggcgaccg tcaaaccagc
     3601 tcaccctctc agaaatattt tctttcaggg cagacagaac tgccgcccgt atttcacggt
     3661 gtctcataca ccctcccagt cattttcttt tcagcaccag gcgtaactga tgcgtcatgg
     3721 ctttcatcat ctgcgccggt aatttttccc ggtacatccg gtcccgttca cgttcaaagg
     3781 tttctgccag cggtccggca gtcggaatct tcaccacttc gatcggcaga cggtggcgtt
     3841 tcggcctccc tttgctgtca gcgccggtgg acgatgatgc ccacggcata cgctgcatca
     3901 catgccagcg tccgttagcc agccgggtga taaaggcatc cgggatccgt cttttcccca
     3961 caatcagcac gctgccaccg cctttcgtga ccgaacgctc gcctttcttt cgtcgctttc
     4021 tgcgggaaag cagcacgctg gccgtcccca gttttatggc gggcagatta ccggtgttga
     4081 tgatgacctt cgcataaacc ctgtctgcgc tggcccgttg caggcggata cgctcacgga
     4141 tgagacggcg cggaaccgcc agctccctgg caaccgaaga ggccgttttc gcgatgatgg
     4201 actccgccac acggttaagt gtcgtggcgg cagcccgggg aacggcacgg cggtcaattg
     4261 catccagatt tttcatggcc tgcgccagac cttttattgc catactcatt cctgttcgac
     4321 aaaaatccgg ggtttaccgt tgtacgtgtc ataacgggtc accgtcagtg tgcgccccgc
     4381 aaacacaaca acatcatgac gggccggacg gtaccgggct gaaaacacca ccagtgacaa
     4441 ctggctgccc gaaagcgccc ccatctccgc ggactcttcc tccggcatca cgtcgtacac
     4501 gacgccgtta atctccgcct gtttgcccat catccgaacg gtcgccacgt ccatccggca
     4561 acacattcgc gtaaacagat cagacattga tttttaccgc cacagcggcg ctgtttgcag
     4621 gagcattttc ccaggctact cccgctgcca ccgcaccgtc tgcagccagc tgcacaaccc
     4681 cgtccttcag ataaaccacc gcgccggact gaatatcgtc agcagactgt ttgggcagca
     4741 ggaacacgcc ttcggcaaaa ccgtcaccgg cctcaccggc aggaatatcg gtaatggcca
     4801 cggccaccat actgccgacc accaccgcag caccgctcag gatggtctga tctccggcat
     4861 tcaccagttc aatggtggta ccgtcctgta caaaattttt cgccataatg ctgtttctcc
     4921 ggacagcccc tgtggggctg tttttcaggc ataaaaaaag ccctttcggg cagtgattgt
     4981 gataacgcgg ttatcaggcc accgacgaac gcaccagccc gcgccagtca agtggtgcca
     5041 ctccggcatc aatacggatt tttgtggcaa taccgtcagt ggtgaaacct tcctgctgat
     5101 caatgtatgg cgtgtccaca ccatccagcc aggccacttc aatggtgtca gtgccctgtg
     5161 ccgccgccag ataccaggtt ttcggatctg ccgcatcaag acgcgcttct gcaatcacct
     5221 cagcaaagtt ctggatgggg ttaatgacac cggcgtttgc atccgcccct ttcacactgg
     5281 ccgatttgat ggtctggttc gccaccgtct ccagtgccac cggtaccagc ataaaggccg
     5341 gacggatatt cagggcgcga tcgccttctt tctgcaggcg catcatctga cgggccgcat
     5401 ccagtccgga aacagagatc cccccggtgg caatattttt gtgatcggca tggaacagcg
     5461 acttaccgtc ggacagtttc gggttatccg tcagcacctt gtaaaccaag tcaccaatcg
     5521 ttgccttcgc cgcacgcccc atcttcatcg gcacgtccac cagcatattc agatcatcat
     5581 tgataatggc ctggcgggtg atggagaaaa tctccccgta ggtggccaga gcaatggtct
     5641 ccttgcgatc tgaggtggta atgtatttat actccgcccc ctcacgaacc tgacgcagag
     5701 aaccaaaacc gcccatcccc acgcgatacg ctgttttgaa gtctgacagg cgtcccttac
     5761 gggtccactt ctggaaggtt tcttctgatt cctcccagcc ctggatcagc cccttgttcg
     5821 acacatccag caggatattg ccaaaatcag aggtgctgtg cgtaagcgcc agcccgacca
     5881 tctgcatggg gttataactg gccaccccaa taccgcgctc cgtcagtgac atacgagccc
     5941 attcacgcag ggtcatcccg ttataggcgt tatccttctc gacattttca aatccggcac
     6001 gggccagcat cgcctggcgg atcccgtctc ccacaaaatt gccgtttccg gcataaatat
     6061 gggccggtgt gtttttgttg gtcggcgagg actccttgcc catttcattc agcagacgtt
     6121 cacgggccat ttccagcgaa cagtcaggat cagccacgca ctgtgcctga agcgtctgat
     6181 agcgaccgcc gaacatggca aacagatcgt taatgcctga catgcgggct ttctgctcag
     6241 ccataacgcg ggcgcgaatg gtcgcctcat cagacactgc cggtaccggt gatggttctg
     6301 ttaccgccgg tgcagggatt gtcactgtgg tatcacgcgg ggcactgttg cgtggtggag
     6361 taatcatgtt tcggatggat tccggcatct ttttaaattc ctctgtacgt tttgactgaa
     6421 tacatgccat tgcctcaaca gcgggtgtca cctggtcagc aaatccgtgt gccagacatt
     6481 cggcaccgga catccaggtt tcatccgcca gcatggcggc aatttcatcg gtggtttttc
     6541 cggttttctg cgcataggct ggcaacagta ccgattcgac tttatccagc aaatcagcat
     6601 aactgcgcat atcctcagca tccccgccac tgaatcccca tggcttatgg atcatcatga
     6661 aggcattttc cggcataatg accgtatcac cggccatcgc aatcacagat gccatcgagg
     6721 cggcaacgcc atccacatac acggtaatgg tcgccccctg atttttcagg gaattaaaaa
     6781 tggcgatgcc ttcaaagaca tcgccacccg gtgagttgat gtggagatta atgtgggtga
     6841 tatcacccag agcattcagt tcgctgacaa actgcttcgc ggtaactccc cagaaaccaa
     6901 tctcgtcata aatataaata tccgcgtcac ccggcccccc agcctgcatc ctgaaccagg
     6961 atttattctt catgctggct ttcggtgtca cgctgatact gtcgttcagt tccggcactg
     7021 ttgcctcctt tgtcgttgac ggggtcagta tcaaagacca gccccagtct gctgttttca
     7081 tcaatttcag ccttgcggcg acgtttgacc tcatccggat tgcgcccgcc ggcacgcacc
     7141 cagtcagatt ctgtcgctgc accaccccgg atctgaattc tccaggcttc agcttcctta
     7201 accgggtcga tccacggcat caccggaccg gaatacgtcg cgttatatag cgttttcatc
     7261 tccacatccg ccggaatttt cagcagacct gccgcaacca ccatattcag ccatgtccgg
     7321 tacaccgggc gggttaccgc gccaataaaa cagtcctgca ggatcaggta accatccgtg
     7381 gactcgacca gctcctgccg ctgggcgctg taggtgccgt tatagttacg cgccg
//
LOCUS       DAZFRN010000209         7273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFRN010000209 DAZFRN010000000
VERSION     DAZFRN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.211"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(334..1470)
                     /locus_tag="ACHKUB_004588"
     CDS             complement(334..1470)
                     /locus_tag="ACHKUB_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6657504.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1473..1835)
                     /locus_tag="ACHKUB_004589"
     CDS             complement(1473..1835)
                     /locus_tag="ACHKUB_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6657505.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2372..4285
                     /gene="mtlA"
                     /locus_tag="ACHKUB_004590"
     CDS             2372..4285
                     /gene="mtlA"
                     /locus_tag="ACHKUB_004590"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO6657506.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4515..5663
                     /gene="mtlD"
                     /locus_tag="ACHKUB_004591"
     CDS             4515..5663
                     /gene="mtlD"
                     /locus_tag="ACHKUB_004591"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6657507.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     QVKRKEQGNESPLNIIACENMVRGTTQLKSHVINALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5663..6250
                     /gene="mtlR"
                     /locus_tag="ACHKUB_004592"
     CDS             5663..6250
                     /gene="mtlR"
                     /locus_tag="ACHKUB_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO6657508.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6262..6471)
                     /gene="yibT"
                     /locus_tag="ACHKUB_004593"
     CDS             complement(6262..6471)
                     /gene="yibT"
                     /locus_tag="ACHKUB_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO6657509.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6756..7118
                     /locus_tag="ACHKUB_004594"
     CDS             6756..7118
                     /locus_tag="ACHKUB_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO6657510.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT         1851 a         1826 c         1838 g         1758 t
ORIGIN      
        1 ctacattgtg atctatagat taaatatttt ttaaatgtga ctgtcatcct tcagataata
       61 aagccatcat cgcaatagat ggaaaagcac tcctgctctt ttttgattag agccgtcgca
      121 ggaggaacaa ctcatatcat cattgcgatc tcgacgatgt atagtctcat cctgcggcag
      181 aacaagacgg ataaaaaatc caatgagagc acactcatcc agaacttatt aacataatga
      241 atattaaaga aaaatcatcg cagctaatga agtaatatgc aaaaataatt agtccttaca
      301 aggagcaaag ttccataatc tcccccctcc ccctcaatga tccaaataaa gataatgcat
      361 ccagctggtc attcttagca gcactttccg catcaccgaa acatggctga aatggtcgga
      421 gtaaaccgcc acctgggcgt agatgccgtc gggtaaggca tcgatatcat cgttagggtc
      481 cagttcaatg gttcccagca caccgtccgt gccgggcacg accgttaatg attgcaatac
      541 cccctgcgcc tgataagaac cgcctggcac gacaggtaaa atactagtca gtttgccgtg
      601 aaacacctgc ccaggtagcg cgttaaacac cacttccgca tcatcgccgg gtttcagacg
      661 taacagcgag ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaagac
      721 catcaccgga cgcagcggca aggcagctgc gtatgtacct gggcggatca gtacctgagt
      781 aacgtagcca ttgctcggcg cgcgaatgac agtctgctca aggttatatt ttgcttcagt
      841 aagttgcgct cttaagctca caatctgcga ttgctcgccg ttaaccatac tgtcgagctg
      901 gctctggatc tgcgcctgct ccgccaccga gcctttcacc agcgcatcct gcgcgaggaa
      961 attttgccgc gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc
     1021 tttcaggtaa cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt
     1081 gttggcctgc gcttcagtga gctgcgcgcg cagcgtcttt atattatgcg tcgccgtcat
     1141 caggtcagcc tgaagtctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag
     1201 cacctcgccc ttttgaataa gctgattatt cttgtcagtg acttcagtaa caattcccgt
     1261 cacctgtggc gtgataggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg
     1321 gttgtagttc atcaacaaaa tcaaaccact caccagaaac acgcctccca gcgccgccgt
     1381 cgccagcgtc cactgattta ccggaatgcg gaagatttta aagaccgccc acgccagcgc
     1441 cacgtaagtt aaaacaatca atagatccat aattagatct ccgggaacgt ggaatagtca
     1501 gtctttttct cggcggcgag ttggtgctcc agccgggaaa ttcggtcaga aagtgcggcg
     1561 atttccgggt cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc ccagccacgc
     1621 tccggttgat agagcgtcgc ccagatccac agaaacggcc agataacatg cagagtaaac
     1681 aggctcaccc agcccgccgt atgaatagcg tcggcatggg ggtgattgcg ctttttggca
     1741 atcagataag ggatatcatg tatcgcgatg attccataaa aaatcaccag aaagacgaag
     1801 atcagcactc ccaacgcgaa atagtttaga aacatatctg cctcggattc acgtttatca
     1861 gtgttgtttt ttggctggca gccagaaggg agtcaggctg atattttgac aataatccgg
     1921 gttcgcgatt ctcgccataa caccaaagaa taatttttag aggtgatgag ttgcttagtt
     1981 acataacgat tttatgacga aggcataaca tgctgtagat cacatcaggt gaagggcgta
     2041 agaaaatatc ttgtgattca gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga
     2101 cattactcac atttaaatgc catttttagc gaaaatcgcc gccttgttgc ttttttacac
     2161 aagcgttttg tgatgaacat cacgtcaatt acctctctac cccctatatt tatgtgactg
     2221 atatcacaca aaaggccgtc gactggacag ttaaccgatt cagtgccaga tttcgcagta
     2281 tctacaaggt ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg
     2341 cgcgacagca aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca
     2401 aagctttggt cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg
     2461 gggtatcatc accgcgttat ttattccaac agggtggtta ccgaacgaaa cgctggcgaa
     2521 gctggtcggg ccgatgatca cttatctcct gccgctgctg atcggttata ccggtggtaa
     2581 gctggtaggc ggcgaacgtg gcggcgtagt cggtgccatc accaccatgg gcgttatcgt
     2641 cggcgcagac atgccgatgt tcctcggttc tatgattgca ggtccgctgg gcggctggtg
     2701 cattaagcac ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt
     2761 gaataacttc tccgcaggca tcatcgggat gatcctcgcc attctggcat tcctcggcat
     2821 tggcccgatt gttgaagccc tgtccaaaat gctggctgcg ggcgttaact tcatggttgt
     2881 ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa
     2941 caacgctatt aaccacggta tcttctcgcc gctgggtatt cagcagtccc atgaactggg
     3001 taaatcaatc ttcttcctga ttgaagctaa cccaggtcca ggtatggggg tgctgctggc
     3061 gtacatgttc tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg caatcatcca
     3121 cttcttgggt ggtatccacg aaatctactt cccgtatgtg ctgatgaatc cgcgtctgat
     3181 cctggccgtt atcctcggcg gtatgactgg cgtgttcacg ctgactatcc tgggcggtgg
     3241 tctggtttct ccggcatctc cgggttctat ccttgctgta ctggcgatga caccaaaagg
     3301 tgcttatttc gctaacatcg cgggtgtgtg tgcggcgatg gctgtctcct tcgttgtctc
     3361 tgctattttg ctgaaaacca gcaaagtgaa agaagaagat gatattgaag cagcaactcg
     3421 tcgtatgcag gacatgaaag ctgagtctaa aggcgcatct ccgctgtctg cgggcgatgt
     3481 gactaacgac ctgagccacg tacgtaaaat catcgttgcc tgtgacgccg gtatgggttc
     3541 cagtgcgatg ggcgcaggcg tgctacgtaa gaaaattcag gatgcaggtc tgtcgcagat
     3601 ttctgttact aacagcgcga tcaacaacct gccgccagat gtggacctgg tcatcactca
     3661 ccgtgacctg accgaacgcg ctatgcgcca ggttccgcaa gcacagcata tttcgctgac
     3721 taacttcctc gacagcggcc tgtacaccag cctgaccgaa cgtctggttg ctgcccagcg
     3781 ccatactgaa aacgaagtga aagtgaaaga cagtctgaaa gacagctttg acgattccag
     3841 tgctaacctg ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag cagcaaccaa
     3901 agaagaagcg attcgttttg ctggcgagca gttggtgaaa ggtggttacg ttgagccgga
     3961 gtacgttcag gcgatgttgg atcgtgaaaa actgaccccg acttaccttg gtgagtccat
     4021 cgcggtaccg cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg gcgtggtgtt
     4081 ctgccagtac ccagaaggcg tgcgcttcgg tgaagaagaa gatgacattg cccgtctggt
     4141 gattggtatt gctgcccgta acaacgagca cattcaggtt atcaccagcc tgaccaatgc
     4201 actggatgat gagtccgtca tcgagcgtct ggcacacacc accagcgtgg atgaagtgct
     4261 ggaactgctg gcaggtcgta agtaatccaa tcccaccctc tccacatgga gacggtgggg
     4321 ctgattgcct gatgcgcttc gcttatcagg cctacaggat gcatcacaat ttgttgaatt
     4381 tgcacgttct tgtaggccgg ataaggcgtt tacgccgcat ccggcgctgc acctctcctc
     4441 atggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta aaaacattga
     4501 tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt cgtggcttta
     4561 tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc aatcaggtgg
     4621 tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggttggt gaaaccgagc
     4681 aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat gtcgttgatc
     4741 tcattgctca ggttgattta gtcactaccg ccgttggccc ggttgtgctg gaacgtattg
     4801 ctccggcaat cgccaaaggg caggtgaaac gtaaagaaca aggtaatgaa tccccgctga
     4861 acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaagt catgtgatca
     4921 acgccctgcc ggaagacgcc aaagcgtggg tagaagaaca cgttggcttt gtcgattccg
     4981 ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg gaagtgacgg
     5041 tagaaacctt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca ctgccgaaca
     5101 tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa ctcttcaccc
     5161 tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat cagaccattc
     5221 gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagcg
     5281 gcgcggtact gatcaagcgt tacggctttg acgcagacaa acatgcggcg tacatccaga
     5341 aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc gtaggccgtc
     5401 agccactgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc ggtacgctgg
     5461 aatacggcct tccgcacaag aatctgattc aggggattgc tggcgcaatg cacttccgca
     5521 gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa ggtccgcagg
     5581 cagcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc gaggcggtaa
     5641 ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc gcccgaataa
     5701 cagattgtca gatatgcagg caacaatgga acaaacccag gcctttgaaa accgtgtgct
     5761 tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg tcgagctcct
     5821 gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt acgcggtgaa
     5881 gtatgctgta gaaccgttac tcgacggcga tggtccgctg ggcgatcttt ctgtgcgttt
     5941 aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg cggaactgct
     6001 gatggcattg cgtgaagaac taaatcacga cggcaacgag tacgccttta ccgacgacga
     6061 aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc cgccacagtt
     6121 tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg
     6181 atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa aaaaagcctt
     6241 tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat aaagccgacg
     6301 ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc cggacggaat
     6361 cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg atgccatgaa
     6421 atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca tgatgatatc
     6481 tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt tcccggtaaa
     6541 gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt ttattatctt
     6601 ttttctatag atttcttaat taatcaataa attgcgtagt ttttaagcat gttaatgatt
     6661 acgttacaga actattgcaa tgtcatcagt tgtgaacaaa gcacctggtc gcgcatacta
     6721 ggggctataa atttatcttt atcagaagcc atcacatgaa agaagtcgaa aaaaacgaaa
     6781 tcaaacgtct cagcgatcgt ctggacgcca tccgccacca gcaggccgat ctgtcgctgg
     6841 ttgaagccgc agacaaatat gccgagctgg aaaaagagaa agccacgctg gaagcagaaa
     6901 ttgctcgcct gcgtgaggtt catagccaaa aactgagtaa agaagcgcaa aaactgatga
     6961 agatgccgtt tcagcgcgca attaccaaaa aagagcaggc tgatatgggc aagctgaaga
     7021 aaagtgttcg cggactggtg gttgtgcacc caatgaccgc actgggccgt gaaatgggcc
     7081 tggaagagat gaccgggttt tcaaagaccg cgttttaagc ccctcagacg actggtacga
     7141 tcgtatcaac ccgcatctga cttgcgttag ctggtcaatg cggggactct cctgtctact
     7201 cacctaatta ccaaacaatt cactcccctt acgtcgattt accgctggtt gatattctga
     7261 aaatgcatct tta
//
LOCUS       DAZFRN010000210         7197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFRN010000210 DAZFRN010000000
VERSION     DAZFRN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.212"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            6..368
                     /locus_tag="ACHKUB_004595"
                     /pseudo
     CDS             6..368
                     /locus_tag="ACHKUB_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(337..2073)
                     /gene="aceK"
                     /locus_tag="ACHKUB_004596"
     CDS             complement(337..2073)
                     /gene="aceK"
                     /locus_tag="ACHKUB_004596"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO6657511.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2354..3658)
                     /gene="aceA"
                     /locus_tag="ACHKUB_004597"
     CDS             complement(2354..3658)
                     /gene="aceA"
                     /locus_tag="ACHKUB_004597"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO6657512.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3688..5289)
                     /gene="aceB"
                     /locus_tag="ACHKUB_004598"
     CDS             complement(3688..5289)
                     /gene="aceB"
                     /locus_tag="ACHKUB_004598"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO6657513.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(5558..6487)
                     /gene="metA"
                     /locus_tag="ACHKUB_004599"
     CDS             complement(5558..6487)
                     /gene="metA"
                     /locus_tag="ACHKUB_004599"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO6657514.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6644..7087
                     /gene="yjaB"
                     /locus_tag="ACHKUB_004600"
     CDS             6644..7087
                     /gene="yjaB"
                     /locus_tag="ACHKUB_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6657515.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         1739 a         1847 c         1859 g         1752 t
ORIGIN      
        1 gataaaaaaa tatgaagcac ctcctcctgg caaataattc taatgaatat cccggtttgt
       61 tttcagcgat acagcataaa caacaaaacg ttgtagagac ggtttatctt gctttatctg
      121 accatgcacg cctgtttgga tttaccgctg aagatattat ggatttttgg caacacaaag
      181 ccccacaaaa atactctgcc tttgagttgg cttttgaatt gggtcaccgg gttattgctg
      241 aattaatcct taatacatta aataagatgg ctgaaagctt tggctttacg gataaccctc
      301 gatacattgc ggagaaaaat tatatggaag ctttactcaa aaaagcatct ccccataccg
      361 tacgctaaat ctttgcctgc gccgatacgc ataaacatct tccacatgcc cttcacgtat
      421 gcggttttgt agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa
      481 cagcggacca atacgcgggt cggcgcatag ccagtggcga aactcttccg ggaaaacatc
      541 gcccggcgag acgctgtacc acggttcgct ggcaagttcg tcttccggat agcgcggcgg
      601 cgggatgtcg cggaaattca cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac
      661 cacacgcccg tgacgggtga caccgaagtt tttaaacagc atgtcgccag gaaaaatgtt
      721 agcagcggca agctggcgaa tagcgttacc gtattcttca atggcgtcgc gcaactgctg
      781 accttccact tgttccagcc agatattgag cggcaccatc cgccgctcga tatagagatg
      841 gcgaattaca atctgctcac caagatcggt gattttttcc gctgcttcct ggagcagtaa
      901 ttccattaat gccggggaaa tatgccgctt ctccagcaca aagttttcaa actcctgggt
      961 gtccgccatt cggcccacac ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg
     1021 agcggcagac atctctttct gcggcgcgaa cttgtctttg atgactttga atacccgatc
     1081 aaagcccggc agcgtaaata ccaacatcac cattccacga atacccggcg cttcaatgaa
     1141 ctgctcatta cagccctgta gataaacgag atattcgcgg tagctttcgg ttttggcgtg
     1201 cttctggcag ccgatagcca tatacaattc agcagtggtt ttacctggca gaatttcccg
     1261 tagccactcg accagcgctg cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa
     1321 gccaaaaaca atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc
     1381 gtcgtccgtc tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt
     1441 gcccaccagc caggcggctt tattgcggta aaacagttcg ttcgccacct gtaaatgact
     1501 ttccgcgagg ttgtctgtcc ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc
     1561 acggctttta ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag
     1621 agattcccag ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa
     1681 gcggcgctct ggctgagagc taaagataaa aagccgctcg ggagtaagcg agcggtggtc
     1741 aaataaccga cagtacacgg agttaaaaaa gctctccgca atctcgaagc gcgggtaatc
     1801 cggcaacagc cgcgtgtaat gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg
     1861 gccgttagta atgcagcgca gttgctccac gaccagacca acgtgatgat cgtaaagatg
     1921 gatacggttt ttcatcgcct gctggacagc atgccagtcg gcctgttcga aacgctgctg
     1981 cgccccggaa gtcacttcga ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt
     2041 ttgagcaatc agtaattcca ggccacgcgg catttagcgc cctcatcagg agcagagaat
     2101 tgccggatgc ggcgtaaacg ccttatccag cctacgttcg gcaacggttg taggcatgat
     2161 aagacgcgca agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc
     2221 ttatccggcc tacgttcggc aacggctgta ggcatgataa gacgcgcaag cgtcgcatca
     2281 ggcatcgtgc accgattgcc ggatgcggcg tgaacgcctt atccggccta cagtcagcaa
     2341 cggttgttgt tgcttagaac tgcgattctt cagtggagcc ggtcagcgcg gtgactgaag
     2401 acgtgccgcc ctgaataata gtcgtcactt tatcgaagta acctgtaccc acttcctgct
     2461 ggtgagatac gaaggtatag ccatctttcg cggcggcaaa ttccggctgc tgcactttct
     2521 caacgtagtg cttcataccc tcgccctggg cataggcgtt tgccaggtca aacatgttga
     2581 accacatgct gtggatacct gccagggtga tgaactggaa cttgtagccc atatccgaca
     2641 gctgctgctg gaagctggca atagttttgt cgtcgaggtt tttctgccag ttgaacgacg
     2701 gcgagcagtt ataagccagc agtttgcccg gatatttcgc gtggatagct tgtgcaaagc
     2761 gacgcgccag ttccagatcc ggcgtggagg tttcacacca gaccaggtca gcatatggcg
     2821 catacgccag gccacggctg atcgcttgct caatgcccgc atgagtacgg aagaagcctt
     2881 cactggtacg ctcgccagta ataaattcac tgtcatacgg gtcgcaatcg gaggtaatca
     2941 gatccgccgc atcggcatcg gtacgagcaa ccagcagggt tggaacgccc gtcacgtcag
     3001 ctgcaagacg cgccgcgacc agtttctgaa tagcttcctg agttggcacc aaaactttgc
     3061 cgcccatgtg accgcatttc ttcactgacg ccagctgatc ttcgaagtga actgccgctg
     3121 caccggcttc aatcatcgct ttcatcagtt caaaggcatt caggacaccg ccaaaaccgg
     3181 cttccgcatc ggcaacgatc ggcaggaagt aatcgacata gcgcggatcg cccggctcaa
     3241 tgcctgcgga ccattggatc tgatcggcac gacggaaggt gttgttgatc cgctccacca
     3301 cagctggcac cgagtttgcc ggatagagcg actgatccgg atacatgctg gccgccaggt
     3361 tagcgtccgc cgctacctgc catcccgaca gatagactgc ttcaataccc gctttcgcct
     3421 gttgcagcgc ctgaccgcca gtcagtgcac cgaggctgtt gatgtagcct tttttcgact
     3481 cgccatgcag cagacgccac attttcgccg cgcccagttg cgccagggtg cattcaggat
     3541 tgactgaacc gcgtaatttc accacatctt ccgcgctgta tgggcgagta atgccttccc
     3601 aacgcggttg agtccactct ttctgtaatt cttcaatttg ttgtgtacgg gttttcatgt
     3661 gcagatgctc catagttatg tggtggttta cgctaacagg cggtagcctg gcagggtcag
     3721 gaaatcaatt aactcatcgg aagtggtgat ctgttccatc aagcgtgcgg catcgtcaaa
     3781 acgcccctgg gagaaacgtt cttcgcccag ttcgctggca atgactttca tctcttcacc
     3841 cagcatctgg cggaacaagg ctttggtcac cggtttgcca ttgctcaacg ttttttgatg
     3901 atggatccac tgccagattg aggtacggga aatttcagcc gtcgccgcat cttccatcag
     3961 gccataaatc ggcacgcagc cgttgccgga gatccatgct tcgatgtact gcactgccac
     4021 gcgaatgttg gcgcgcatac cttcttcggt gcgttcacca tcacaaggtg ccagcagctg
     4081 atcggcagta atcggcacgt cttgttcgcg catcacttca agctgatttt tacgggagcc
     4141 gagaatgtcg ttgaataccg ccattgccgt atccgcaagg cctgggtgag caatccatgt
     4201 gccatcgtga ccgttattgg cttccagcgc tttatccgct tttactttgt tgagcacctg
     4261 gttattgcgc tcttcatctt tgctcggaat aaacgctgcc atgccgccca tcgcaaaagc
     4321 accgcgttta tggcaggttt taatcaacag gcgtgagtaa gcattcagga atggtttatc
     4381 catcgtcact gcctgtctgt ctggcaggac gcgatcggga tagtttttca acgttttgat
     4441 atagctgaag atgtaatccc aacgaccgca gttcagacca acaatatggt cacgcagcgc
     4501 gtgaaggatt tcatccatct ggaacacggc gggcagcgtt tcaatcagca acgtcgcctt
     4561 gatggtgccg cgcggcagat taaagcgatc ttctgcatag ctgaagactt cgctccacca
     4621 ggccgcttcc tgccaggact gggttttcgg cagatagaaa tagggaccgc tgccctttgc
     4681 caacagtgcc tgatagttgt ggaagaaata gagcgcaaaa tcaaacaggc taccggggat
     4741 tgcctcacca cgccaggtga catgtttttc cggcaagtgc agaccgcgta cccgacaaat
     4801 caaaaccgct ggattgggct tgagctgata aattttgcct gcttcattgg tatagctgat
     4861 ggtgccgtta accgcatcgc gcaggttaat ttgcccgtcg atcactttgt tccagtctgg
     4921 tgccagtgaa tcttcgaaat cggccataaa gactttcaca ttggcgttca gtgcgttgat
     4981 caccatcttg cgctcaaccg ggccagttat ctcgactcga cgatcttgta agtccgcggg
     5041 aataccacga attttccagt ccgtatcgcg aatggaagct gtttccgaaa taaaatcagg
     5101 caaagttccg ttatcaatat cttgctgctg ctgaatgcgc gctgccagaa gtttattgcg
     5161 ttgtggcgta aaatgcgtca ccagctcagt cagaaattct accgcttcgg cagtaagaat
     5221 ttgcttctcc tgctcgccat acggccttat gaaagccagt tcatcggttg ttgttgcctg
     5281 ttcagtcatc gtgcagctcc tcgtcatgga tccgaaaact tccccactgt gaacgaagga
     5341 tcgttgtgcg cttttcgttc agcacaacta agactactca tttaaaatgc aaaatcaaaa
     5401 acaatttcca ttttaattaa aaatacttga taacttattg ataacaatat gaataaaatt
     5461 tattcacctc ctgaggtgcg tttcggaaat aaaaaaggca cccgaaggtg cctgaggtaa
     5521 ggtgctgaat cgcttaacga tcgactatca cagaagatta atccagcgtt ggattcatgt
     5581 gccgtagatc gtatggcgtg atctgataga cgtaatagtt gagccagttg gtaaacagca
     5641 aattaccgtg actacgccag ctcgctcgcg gtgtattttg cggatcattg tgcgggaaat
     5701 agttatacgg tacttccggg cctagtccgg cttccacatc gcggaaatat tcctgcgcca
     5761 gcgtttgcgc atcatattcg ggatggcccg tcacaaaggc aatgcgctta tctttgctgg
     5821 caaacagata tgcatcccct tcttccgtct ctgccagaat ttccagatcg gtgtaatcac
     5881 gaatcaacgc tgccggaaag tcagcatagc gcgaatgtgg tgccaggaat gaatcatcaa
     5941 agccacgcgt cagaagcgca tgaggatgga gaatatgatg ctcgtaaacg ccagagagtt
     6001 tgtcggtgcg agtttgctta ggaatgccgt agaggatatt gagcgcggcc tgtaccgccc
     6061 agcagacaaa cagcgtcgag gtgacgtgat ctttcgacca ctccagcacc tgtttgatct
     6121 gcggccagta agcgacatca ttaaactcca ccaggcccag cggcgcaccg gttacaatca
     6181 gaccgtcaaa gttctgttcc tgaatatctt caaagttaca gtagaagttg ttcagatgct
     6241 ctgcgggcgt gttgcgcgat tcacgggaat cgatgcgcaa cagctgaata tcgacctgca
     6301 aaggtgagtt tgaaagcagg cgcagaaact gattttcagt ttcaatcttc ttcggcatca
     6361 ggttaaggat cagaacctta agtggacgaa tttcctgacc agacgcacga gaagttgtca
     6421 tcacaaagac gttttcttca cgcaagaaat tgacggcggg tagctcgtcc ggcacacgaa
     6481 taggcataac ctgattacct cactacatac gcttatacgt ttagacatcc agatagctga
     6541 agataaccag aaaatttgcc aatgtcgagc ctgcatgttg aaggtgagaa agtttcaagg
     6601 aaaaggctac gttagaatat aagaatgacg aaaaggagag aaaatggtta ttagtattcg
     6661 ccgctcacag catgaggaag gggaggaact cgttgcgatt tggtgtcgat ctgtcgatgc
     6721 cacccacgat tttttatcag cagagtatcg ggccgagctg gaagagctgg ttcgttcctt
     6781 tctgccggaa gcaccgttgt gggtcgcggt taatgaacgg gatcagccgg ttgggtttat
     6841 gttgttaagt gggcagcata tggatgcgct gtttatcgat cctgatgtgc gcggctgcgg
     6901 cgtgggtcgg atgctggtgg agcatgcgct ctcgatggca ccggaactga caaccaacgt
     6961 taatgagcaa aatgagcagg cggttgggtt ctataagaag gtgggtttta aggttacggg
     7021 acgctctgag gtggacgatt tggggaaacc gtatccgttg ctgaatctgg cgtatgtggg
     7081 ggaataagcc gcccctgccg tgcctgccgt gcctgcccag gctggagaag acgcgccagc
     7141 gtcgcacccg gcattttttt caccaacgca aaaaggccat cgtgactgat ggccttt
//
LOCUS       DAZFRN010000211         7154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFRN010000211 DAZFRN010000000
VERSION     DAZFRN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.213"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(81..716)
                     /gene="msrQ"
                     /locus_tag="ACHKUB_004601"
     CDS             complement(81..716)
                     /gene="msrQ"
                     /locus_tag="ACHKUB_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO6657516.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(717..1721)
                     /gene="msrP"
                     /locus_tag="ACHKUB_004602"
     CDS             complement(717..1721)
                     /gene="msrP"
                     /locus_tag="ACHKUB_004602"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO6657517.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1830..2243)
                     /gene="hiuH"
                     /locus_tag="ACHKUB_004603"
     CDS             complement(1830..2243)
                     /gene="hiuH"
                     /locus_tag="ACHKUB_004603"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO6657518.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYYKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2376..3047
                     /gene="hprR"
                     /locus_tag="ACHKUB_004604"
     CDS             2376..3047
                     /gene="hprR"
                     /locus_tag="ACHKUB_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO6657519.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3047..4405
                     /gene="hprS"
                     /locus_tag="ACHKUB_004605"
     CDS             3047..4405
                     /gene="hprS"
                     /locus_tag="ACHKUB_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO6657520.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4513..5364)
                     /gene="hchA"
                     /locus_tag="ACHKUB_004606"
     CDS             complement(4513..5364)
                     /gene="hchA"
                     /locus_tag="ACHKUB_004606"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO6657521.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMKIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(5956..7071)
                     /locus_tag="ACHKUB_004607"
     CDS             complement(5956..7071)
                     /locus_tag="ACHKUB_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO6657522.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNYD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADREGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
BASE COUNT         1958 a         1539 c         1678 g         1979 t
ORIGIN      
        1 tgatgtgatc tgcgtaggcc ggatagggcg ctcgcgccgc atccggcagg gataacgcgt
       61 actttgtcgg caatatgaag ttatacagat aatttgttgt gaacctgctt gcgtaaccgg
      121 ttaaaaaggg aacgcaactt cttataccgt aaggctaaaa gcagtacagc cagcccagcg
      181 tagatgagcg gctgcggtga aataatcttc actgaccaca gataatgtat tggggccagg
      241 atcgcgacaa gatagacgaa attatgcaac tgttgccagt gcttgccgag ttttcgctgc
      301 atcgcctggg ttgaagtaaa cgctaaagca agcaaaataa cccagctgat aattcctaac
      361 gttaaataag gccgggtaat taactccttg cctaataacg ccagattatt cacgcccagc
      421 tccagcaatg cgtaactggt taaatgcagt gtcgcccagg caaagcacca taagcctaac
      481 aggcggcgag tgcgtatcaa taacggctgt tttgcgtagc gtgccagagg tgtgattaac
      541 aaggtcgcca gcaaaaattt cagtgcagtg cgaccggtaa aatgctgaat atctttcacc
      601 ggatcggcac ccagtccacc gtgattgatc gcccagacca gccagagaaa cggcaacaat
      661 ccggcaagat gcaggctaac tttcagccat gtcacctgtt ttgctgtcag acgcattcag
      721 aaattctccc gcaaatccag gccacgatac agcgatgcca cctggtcggc gtaaccatta
      781 aacagtagcg ttggctggcg ctgaacatcg aggatgccgc ctgaaccaat aaatcgttcg
      841 gtagcctgtg accagcgcgg gtgatcaaca tgcggattaa cgttggcgta aaaaccgtat
      901 tcgtcaggcg ctgccagatt ccaggtggtt ggcggacgct cgcgggtcag cttaatactg
      961 actatcgatt taatcccttt aaagccatat ttccacggca caatcagtcg taccggcgcg
     1021 ccattttgtg gcggtaacgc cttgccataa acacccacgg tcatcagtgt gagcggatgc
     1081 attgcttcgt cgagacgcaa tccttcgaca taaggatatt tcagcccgcc gccgataaag
     1141 cggtcctgct ggcctggcat ttgttccggt gcataaattg tttcgaaagc gacatacttc
     1201 gcattgctgg tgggttcggc aagcgccagc aatttgtgca gcggaaaacc aatccacggc
     1261 accaccatcg accatgcctc cacgcagcgc atacgataaa tacgctcttc cagcgggaag
     1321 cgacgggtta aatcatcgtg atcgagggtc aatggttttg ccacttcgcc gctgattttc
     1381 agtgtccatg gatcggtttt caggctacca gcattagcgg cgggatcggc tttatccagc
     1441 ccgaattcat agaagttgtt ataaccggag actttatcta ctggcgtcag tggcaggtta
     1501 ttttgccagg cggcaggctt gctgaactcc agcggttttc cggcgggggc gggcgggcga
     1561 tcgttccctt taaaccagct aagcagatcg gcatgcgcag cgtgaggcaa agaaagtgca
     1621 gctgcgctga tgcccagtgc ttttaacacc tgccgacgct tcataaagaa taccgactcg
     1681 gccgtaacat ctgattcttt taaaaattga ttctttttca taagccatca cacttttttg
     1741 tcgttagcca gcaagagccg tttactgtta atctgtcagg gagtgtcctc cctaacagta
     1801 gcagagatct gcttttacat atttatgctt taactgccac gataggttga atacccatat
     1861 tggctaagta ataaaggtac atgataatgc tcgttcactt tattaatatg aaattcaacg
     1921 gggatctcag ggaagaaact ttcaagattt tgtttcttgt aatagtcccc ggttttaaat
     1981 acgacacggt aatcgccagt agtcgcagtt tgctcgggcc acagtgcctt aattcgtcca
     2041 tccttatctg ttttggcggt attaagttgt aaccagccgt tatccgcctt cttttcaaga
     2101 gtgactgtca cgtcagcagc gggttttccg gtttgctggt tcaaaatgtg cacgctaaga
     2161 atgttttgtt gtgcggcata aaccaaagaa ggtaatgaaa atgctgccgt tgctaccgag
     2221 agtactaaat aacgctttaa catgtttata tccttgtcat gtgaatgagt tttgaggata
     2281 gcgaaaaagc ggccagggta accttacagc agcattacaa ttttgtaatg aaccaggctg
     2341 tttctataac atatgattta tggcatatta ttttcatgaa gattttactt attgaagata
     2401 atcaaaggac ccaggagtgg gtaacgcagg ggctttccga agcgggttat gtcattgatg
     2461 ccgtttctga tggcagagat gggctttatc ttgcgctgaa ggatgattat gcattgatca
     2521 ttctggatat tatgcttccg ggtatggatg gctggcagat cttacaaacg ttaagaacag
     2581 caaagcaaac ccctgttatt tgccttactg caagggattc tgtcgatgac agagtcagag
     2641 ggctggacag tggagcaaat gattatctgg taaaaccttt ttcattttct gagttgctgg
     2701 caagggttcg ggcacaatta aggcaacatc acgctttgaa ttcaacatta gaaatcagtg
     2761 gcttaagaat ggactctgta agtcaaagtg tgagcaggga caatatcagt attacactga
     2821 cgcgcaagga gtttcagtta ctttggctac tggcctccag agctggcgaa attataccca
     2881 gaacggttat tgcgagtgaa atttggggaa tcaactttga tagtgatacc aatacggtgg
     2941 atgtcgccat tcgcaggctc cgcgcaaaag ttgatgatcc ttttcctgaa aagctaatcg
     3001 ccacaatccg ggggatgggc tattcattcg tagcggtaaa aaaataatga aaagattatc
     3061 tataaccgtc cgtttaacct tgctttttat attgctgctg tctgttgctg gcgctggaat
     3121 tgtctggact ctctataatg gcctggcaag tgagttgaaa tggcgcgatg atacaacact
     3181 cattaaccgg acagcacaga tcaagcagtt gttaattgat ggggtaaatc cagatacgtt
     3241 acctgtatac tttaaccgga tgatggatgt tagtcaggat atcttgatca ttcatggtga
     3301 tagcatcaat aaaattgtta accggacaaa tgtcagtgat gacatgttaa ataacatacc
     3361 tgctagtgag acaatcagcg cagctggcat ttacagaagc attattaatg atactgagat
     3421 tgatgcttta cgaattaata ttgatgaagt ttcgccatca ttaacggtta ctgtggctaa
     3481 attggcttca gccagacata acatgcttga acagtataaa attaatagca ttataatttg
     3541 cattgtcgcc attgtacttt gctcagtatt aagtccgctg ttaatcagaa cgggattacg
     3601 agagatcaaa aagttgagtg gtgtaacgga agcgctgaat tataacgata gccgggagcc
     3661 tgttgaggtt agcgcattac cgagagaact aaaacctctt gggcaggcgt tgaataaaat
     3721 gcatcaagcc ttagtcaaag attttgaacg cctaagtcaa tttgctgacg atctcgctca
     3781 tgaacttaga acgcccatta atgcattact gggtcagaat caggttacgc tcagtcaaac
     3841 cagaagtatc gctgaatatc aaaaaacaat tgccggtaac attgaagagc tggaaaatat
     3901 ttcgcggtta acagagaaca tactgtttct tgcccgggca gataaaaaca atgttttggt
     3961 gaaactggac tcgctttctc tcaataagga agtcgaaaat ttgttggatt atcttgaata
     4021 cctttcagac gagaaagaga tttgctttaa ggtcgagtgc aatcagcaaa tctttgcgga
     4081 taaaatttta ctgcaacgaa tgttatcgaa tcttattgtt aatgccatta gatattcacc
     4141 agaaaaatcg cgtattcata taaccagttt tcttgatacc aacggctatc ttaatattga
     4201 tgtcgccagt cctggaacga aaattcatga gcctgaaaaa ctcttccgta gattttggcg
     4261 gggagataat tcgcgtcatt ccgtaggtca gggacttggt ctttctttag tcaaagcgat
     4321 tgccgaatta catgggggaa gtgctacgta tcactatctc aataagcata atgtgttccg
     4381 gattacgtta ccgcaaagaa attaacatag ggcttcagta cgccataaat caaccatgaa
     4441 aaaacgcagc cacgtcgtga gtattaacgt ggctgcgatt gattatgcgc ttacattcat
     4501 acgtaacagg gattaacccg cataagctgc cagcatttcc tgcgccgcca gtttacccaa
     4561 cgcattcgct gcgaaaggac tgtcgccggt gagaacttta cggtccttat gtactcgccc
     4621 ggtgatgtcg tcattaatga ttttcatgcc cattttcttc agctcttcgc cgaagtacca
     4681 ggtgagatga cccggcatat agcctatctc tggcgtttgt ttgtctgcgg cgtctgggaa
     4741 tgcgcaaatg gaataaccat tcagtgggtt atcgccgtgg cgaagcgcca gaaaagccgc
     4801 cgggccgtgg caaagggaga taacaaaacg gtcatttttg attgcccact gcaaagcagc
     4861 agctacgtcc tggctttctg gtaaaccaat aagtgcgcca tgaccaccag gaacaaagat
     4921 tgctgcatat tcgctatcag cgttgaggct ggcgacaaca tccgcgagtt tcttcggatt
     4981 gcggaacaac gatttatgct gctcaaagaa tggcattact ttttcatctt tgtgcggcat
     5041 agcccagtat tcaaacttgg tcatcagacc ggaaatggtc gccacttcga attcgaaacc
     5101 tgcagcatgg agatgataca acggcagcaa cgtttcaatc ggatggttac cggtcgagaa
     5161 cagctttccg ttatcggtcg gcaaataacg ttcgtccgcg gcgatcacca gaattttatg
     5221 tttaccgcga tacggttttg gatagtccac gccatcaaga tcagagacgg gactggtata
     5281 ttggctaagc gaatattctg aagggaagaa tgcattatct tcagcaatat cgacctgcgg
     5341 atttttactt gtttgaacag tcatagtgta ttccttattg ttgcttagtt agggtagtca
     5401 ctatatttgc gactgagctg gtcgctattt ttaaccaggc tggaaaattg agtcaatata
     5461 tcgaaagcgg tagtcggcgg gtgtaagtat gtaatgatga tgctgcactg gtgttgaata
     5521 ttattcacgc taaattttcc ttattgagca acgtcgtcgc aagtggcctc aatgatttta
     5581 gaccattatc agaggtacag ggtggcgggg agagatttag tgatgtggaa tattgtggtg
     5641 ctgatgaata tttatatccc ggattggtag agaaaaagca tatttcatgc actatttttg
     5701 caaatatttc tatatgggaa gcgtgtttgt tacggattaa tacataaaga aatgcatttg
     5761 ggtgttggct tgaagattag ctggtagaag atattgtgcc aggaggggca gggtaagggg
     5821 ctgtatcgat atgctttggc aattgcgtgt gccgcagatg gagatgaaat gccaataaaa
     5881 aagcctgagt tcgatgaggg acgaactcag gctagtgtta agttttacag taacacgccg
     5941 cggcgcgtcg gctgattaga actggtagac gataccaact gcaaactggt catcagctgc
     6001 tgcgccagta atggcggcgt catctttatc cagcatgttg aatttgtatg cagtataaac
     6061 gttcatgttc ttattaaagt agtaccaggt accaacttca atgtagttca cacggtcagc
     6121 gtcaacatat ctatgtccag gtaccccttt gatatcttta cctttagagt acacataacc
     6181 taaggacgga cgcagaccaa agtcgaattg atactgaaca accgcttcaa agttctgggt
     6241 ttcattgata agttgatcac gcattattgt attgccttct cgatcggcaa cacctgtatc
     6301 aatagaaccc ggagtcatat tacgagtttc gccgtacatt acagctgcgt agacattgtt
     6361 cgcgtcatac tttgcagcca acgaccacag ttcagcattt gaaccgtttc catcggcaac
     6421 ttgatcgttg gtgcgatcag atttgctata tgcaccaacg ataccaaaac cattgatgtt
     6481 atagtctaca gacaacgcat agccgtcacc attttgtttc ttagtgctac ggttgctgtt
     6541 tttgccttgg tactgaatcg caaaatcaag accatcaacc aggccaaaga aatcatagtt
     6601 acggtaggtt gcaacaccgt tggtacgacc attcatgaag ttgtcagcgg aagcccagga
     6661 atcgccaccc cattcaacta acatatcggt ataagctgcg gcatcataag caacaccgta
     6721 gttacggcca tagtcaaatg atcctaactc gttatatttc aaaccggcga aggttaaacg
     6781 ggttttttcc tggttttcac cttcgggctt gctggcgtcg aggttgtatt caaactgacc
     6841 aaaaccagtc agttcgctgg tgatttgggt ttcaccttta acaccgaaac gagcatagga
     6901 ggtatcttcg ttttcgctgt tattatcatc agtatttgac cagatgcgtt cgccaaccat
     6961 cttgccgtag aaatcaactt tattgccatc tttattataa atttcagccg catttgctgc
     7021 gccagcaact aataacgccg ggaccagcat tgccagaact tttcttttca ttatgtattc
     7081 ccttgtgatt ataatcttca tgaatatatc aataagtgcc gttatccaaa aaaaagcaca
     7141 tttggatact attc
//
LOCUS       DAZFRN010000212         7150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFRN010000212 DAZFRN010000000
VERSION     DAZFRN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.214"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(77..418)
                     /locus_tag="ACHKUB_004608"
     CDS             complement(77..418)
                     /locus_tag="ACHKUB_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6657523.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVMTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
     gene            complement(411..3653)
                     /locus_tag="ACHKUB_004609"
     CDS             complement(411..3653)
                     /locus_tag="ACHKUB_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6657524.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQCRALAELNSQLTEIRASAVGMTSAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFALSQKVLMDISQRTGTAFSDNATLFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEELAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLVMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQSALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSGAEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREETLKNDNADARASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(3701..3910)
                     /locus_tag="ACHKUB_004610"
     CDS             complement(3701..3910)
                     /locus_tag="ACHKUB_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO6657525.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(4006..4380)
                     /locus_tag="ACHKUB_004611"
     CDS             complement(4006..4380)
                     /locus_tag="ACHKUB_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6657526.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESVRKK"
     gene            complement(4395..5111)
                     /locus_tag="ACHKUB_004612"
     CDS             complement(4395..5111)
                     /locus_tag="ACHKUB_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO6657527.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(5177..5521)
                     /locus_tag="ACHKUB_004613"
     CDS             complement(5177..5521)
                     /locus_tag="ACHKUB_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO6657528.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(5518..5964)
                     /locus_tag="ACHKUB_004614"
     CDS             complement(5518..5964)
                     /locus_tag="ACHKUB_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO6657529.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGTENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKVDNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(5961..6311)
                     /locus_tag="ACHKUB_004615"
     CDS             complement(5961..6311)
                     /locus_tag="ACHKUB_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6657530.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(6321..6647)
                     /locus_tag="ACHKUB_004616"
     CDS             complement(6321..6647)
                     /locus_tag="ACHKUB_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6657531.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(6644..>7150)
                     /locus_tag="ACHKUB_004617"
     CDS             complement(6644..>7150)
                     /locus_tag="ACHKUB_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6657532.1"
                     /translation="DGRDYDPEVLQKAVLDAVSALPAPQDGRDATALEIHPAIDDQKS
                     FPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRT
                     EKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTGDKPGEAHSLAWTLAAKR
                     GRDAGGGK"
BASE COUNT         1460 a         2381 c         1684 g         1625 t
ORIGIN      
        1 gatttcgcac tttctgacaa gacagattaa aaataattaa caactaaaca ctaaagaatg
       61 atttatagga taattctcat ctaactacct gctctatcgt gcaactgaaa tcactgtacc
      121 gggcgttatc cgtaatactc cactcccggc acaccaccct gaccgtccgg ttatgtttcg
      181 ggggcttcca caaaaaagcc cggtaaccac catgccatga cagaaacgcg gacagtgcct
      241 cccgctcagc atccgttgtc acccgaaaaa ccacctgaaa ggtcttcagt tgcgcattca
      301 gtcctgccgg acgacgctgc tgataaccgt cgccaaactt caccgtcatc accgacggtt
      361 tctccgtcac ctgcatccct tcccggggac accagtgaag agtcttaatc tcatccactc
      421 agcattcctc cgtcacgacg catggataac atcaccgcct gtacccgctg gtcaatcagt
      481 tgcacaaggg tgcccgccgc ttccggccct atctgcccgt tagcgccgtc attctgaatg
      541 gcgatgtggt acaccgggga atacaccaga cccgcactgc cgttcatact gcccaccgca
      601 cgcacgccga gtgaaccatc cgctgcccgg gtcaggggca tgatggcttc aggtccggcc
      661 tcccccatca gccctgcccc ttttgcaaag gcaaagtacg tgggcgtgtc cacaatactg
      721 ttactgtatg cactcaggtt tgccgaggta taaacgccgc cttttgcatt tgccaccgcg
      781 ccacccagcc agttgcctat actgccgaaa aatcctcctg caccggacat actgtttgcc
      841 gccatcttaa tgccgttgac aatggccgca ttcataagaa cttttgatat ttcctgcagg
      901 attgatgcag cccagctgcg ccattccact ttatttccgt tcagcatctc cgtgatgtta
      961 ttcaccatcc ctgagatacc gtctgtcgca agctgtgctg cctgtgaagc gtaatctgat
     1021 gcgctgtcca cccagttact tagcccttcc tgcagccctt tctgccagtc agcacgctgc
     1081 gcatccgatt cggcataaaa agcctcctgg tctttaaggc gttcactcag atactgcgca
     1141 ttctgcgcca gcgcctgtct gtaaaaatcc tcactgatat cccctgtctg atactgagac
     1201 tgcaggtccg catccttctg gcggtagccg tcacgaatct gctgcagctc ccgcatccgt
     1261 tcacggaccc gctcaccctg cccgtacccc agcagctcag cgtcatttga tgcccttgcg
     1321 tctgcattat catttttcag ggtctcctcc cgggcccgta actgctcccg gattttcttc
     1381 tggtcaatca gggcagcatt gcgcagcagc tcctgcttct gtatctccgt cagggttttc
     1441 agctcgccct gcgaggtctg gtatttcagt tttgccagtt ctgtattctt cccggccagt
     1501 gccagttgtt ccttctgctg cttcagcaga cgggaaaaac tgtcttccgc tttttccgcc
     1561 cctgattttc cggcccggga tttgggttta ttcgcctcat tattgcgcca ggcttccatc
     1621 gaattacgga tgtaacgctg cctcgcctcc tgatacgtat cccccaccag accaaggtca
     1681 tccgccgcat accccagccg ggcacgctct ttttcttccc ctttcagcct ggacagggcc
     1741 agttgacgct ctgtgttatt cagggcgctc tgctgtttat catccagagt ggcctgtggc
     1801 agtcgcagcg gtacactcgc cagtccctga cgctgttgca gcagttcatt acccagcccc
     1861 agcaggcggt tgaattccgt atgctgaccg ttcatgacca gcagtgactg atacgcttta
     1921 ttctgctctg ctgcctgctg acgaattaac acaacacggc gctcttccag tccggcaagc
     1981 acatcctgaa tggactgcgc tttttcctgc atctgtgcca gacgggactg ctcaacagca
     2041 aactgctctg ttgcctgagc aagctcttcc gttacagttt tcaccgatgt cagatggttt
     2101 atcatgaagc cgttaccggt cgtccagccc ggattagcca gcacatactg atacccggcg
     2161 attttttcct gcagggattt cacccggctg gcctgctcat caatcagccg gttctgctct
     2221 gtcagcgcct cccgtgttcg cccttcatta tctgaggctt caggcagaga cattgatggc
     2281 gttttatgcg cgatttcatc aatcgtcagt gcatactggc gcgcagactc cctggcctgc
     2341 tcctgattct ggtacagcgt gtaccatgcg gcagctccca gcatcaccag tccgggtacg
     2401 cctccaacca gtcccagcgc accgctcatc agacgtgagc ccaccgccgt tgtactgttc
     2461 agcgcattct gggcggcggt tctggcagca atatttctgt tcaggcgttc ctgtgtgacc
     2521 gccagacggg cctctgccgc aatctgcatc tcagtcccgc gggctgccgc cacagcctgc
     2581 tgagcccggt acacggctgc ccttgcccgc gccgtggcaa tctgcgttcc ccttaactgg
     2641 gcctcagcca gtgccacttc attacgtgca gcggtcacaa gtcctgccgt ggcagacatc
     2701 gctccggaag ccagattacc aaagtaccgg gccaccccga cagcaaccag cacacccgca
     2761 gctgttgcca cggtatcaat atttccggcc acaccgttca gtgcgccgga gagcgttttc
     2821 gtcgctccgc tggcctcatt cgcgccgccc acccaggcca taaaggcgtt ttccaccttc
     2881 gtgatcccgt cagacaccgt ttccggcatg gctgcatatt catcacgcaa tatccccagc
     2941 tggctgatta acgcgggaac gactttatcc gccgtcagtt gaccatcgtc cgccatcgcc
     3001 ttcagatcct tacgggccac gcccatgcct gcagccagtg cgcgaatgat ccggtcccca
     3061 ctttcattga ccgaattaaa ttcctcgccg cgcaacaccc cctgtgccag cgcctggctg
     3121 aactgggtga tcaccgaacc cgcctctgcc gcactggcac cggaaatttt cagccctgtc
     3181 gaaatggcct ccgtcacctt cagcacatca tcagcactgt aaccatattc acgcatcgag
     3241 gcagccgaac gggcaaacag ggtcgcatta tccgaaaatg cggtgcctgt ccgctggctg
     3301 atgtccatca gcactttctg tgataacgca aattcatccg atgactgcga cgcctgtttc
     3361 agacgggcat tcacggagct ccattcatct gccagcgaaa tcaggtgtcc ggtggcaaag
     3421 gcaccggcaa acgcactggt cattccgaca gccgaagcgc ggatttccgt caactggctg
     3481 ttcagctcag ccagagcccg gcactgctcc ctggctgccg cagcagcctg acgtccgcca
     3541 ttctgcaggg tccggtaata ttcactgccc atacgggacg cccgctggat ctccgactgg
     3601 aatgactgtg aatttgccga aattttgata atcagttcac gtaacgtcgc cattcacctt
     3661 tctcctggcg taaaaaaacc gcctcagcgg ttctcatcat tcatgactgt gctgcaaggc
     3721 tcagcgcgtc ttccagcgcc gcaaacggat ccacctccgg cttatcctca tcctcgcccc
     3781 agcagagcat ggcgtccttc agtgcaacat tcatcccctg tgccccgaaa accgctttca
     3841 cgatctgtgc attacggata tccccgcgct catcacccag cggggatatc ctgtcgaact
     3901 ccatccacat catcgcctcg ctcgcactca ggctgtgccg cagttcggat aaggtgcgcc
     3961 ccagacggag cgcaagtcgc atcagaaagc gaatttccgg gcgggctact tttttctgac
     4021 cgactctgca tcagcgatca gttccagtgc ctgacgcagc aagcgggcat gtaccggacc
     4081 atagacggcc agcacctgct cacggtcgtc cggagtgaac acccgttgca gatccgtatc
     4141 acacaggaca tcgcagaaca gcgtcacatc cgcttccagg ttacggcggg ttttcgccac
     4201 caccgacaga gtatcgtcat cctctccatc accattgagc acgtcctgcc acagatacca
     4261 ggcctctgcc gaaggctccc gcagcaccac gctgacatta ccccattccg gcaccttcac
     4321 cgttttatga cgaaaccctg acagtctggc cagcgccagc gttttcagat cctttttcat
     4381 gatgacccat ccccttatcc ggcggctgcg ctcactgtca cggtgcattc aacagacgtc
     4441 acactctgtg ctttctctgc cgaatcggtc accacgcagg tatatttccc cgcatcggcg
     4501 gactgcgcac ctggcttact gaaggtgtct gtcgtctgcc cgtcaaccgg ctgaccatcc
     4561 ttcttccagg cgtatttata cggcggcgtt cccccgttgg cactgactga cattgtcagc
     4621 agcgcaccgg tattcacggt aagtgtctta tccagatttt tcacaaacgc cagcggtacc
     4681 acaaaggaca ccggtttgcc tttcagacgc agtgagaacg ttgcagccac cacgccgttg
     4741 gtaccggatg accaggtgtg ctggcgcact tccgccagga atttaaagcc cttaccggac
     4801 ggaaacagca ccttaaacgc atacaacgcg tcattgtcat aggcatcacg cagggcgttc
     4861 tgggcctgat tcagataaaa attacccgac atggaaatct cagacgacgc ccccagaccg
     4921 ttgatattct cctgctctgt ggagcagagc gtggtcacat caatatcctg tttctgaccg
     4981 gcggtgaact ggacttcctt gatggtgcag tccaggcgca gatattccgc cttatccata
     5041 gtttcagcag tcgccggggc agatgaaatc atcacctgcg tcagctgtga gcgttcatac
     5101 aaagcagaca ttctgcctcc tgataataaa aaacccgcac gcggcggggt atgggttttg
     5161 tagaaaaaag aaaaagtcac accgtgacct gaaactccag ggttgcacgg taacagcggt
     5221 tttccggaat atagtcctgc atttcactga cggatcccgg ggccagcagc attatggctt
     5281 cacgggcgtc ctgacgtatc tgacgcgcct gcgtcacagt cccggcataa acgtctatct
     5341 gcaccgccac tgaggactcc gcctgcccgc ccatcacgtc cgcagacacc gatgaaatca
     5401 ggctgaaaac cacccacgga agcgctaccg acggcctgcc atccagcagg gggaccacat
     5461 acgggtacac ctgcccgccg gcaagatgcg ccagatgagg atacaaatcc gcctccgtca
     5521 tcgtctcagt acctcatcaa tggcccggtt catccgagca atcgccacct gcgctgcctg
     5581 ttcactgcgc acatcaaacg ccgggcgcac aaacgggtgc ggtggcatat tcacggtccc
     5641 catttccaca aaccgccagt agaaagcatt gcgcgggtta tccaccttca tggtgttatc
     5701 gctgttaccg gtgtccggat taacaccacg gatatggaca ccggattcca tcccgccatc
     5761 gcgggagcgc cgggaaagga ccaccacatt gcggcgcagt tttcccctgc gcaccggtgc
     5821 ccgtgacacc acttcttctt tcagcacatt cgcacccgca cgggttgcct cacgcagcac
     5881 ccggttgttt tccgtaccac tcagaagctg caaatcgcgg ctgatgtcct ccagccccga
     5941 aaaatccagc agggtttcga tcatttttcc cctcccagcc gacagagaat ttccagacgt
     6001 ccgccggtcg catccggcac gggcagcccg acaacgttca ggatccggtc acgccatgga
     6061 ccactcagca catgaagtcg tgacgctgcc gtgatttccc gaccggactg accgcgcacc
     6121 cagatgcgga tttccgcctg cgccatttcc gcaccggact gcatccgctc ccggctgctc
     6181 ctgccacgga tatccgcatg aattttcccg catgacaccc attcttccgt catttctccg
     6241 gcagcattac gggttaacac cgggttcaga acacttatca tctgtgtcag acgacctgca
     6301 gatattgcca ttccccctcc tcataacacc gtcggacaac gcaaatcgta aatcagcacg
     6361 gaaacagaaa acggcagctc cccctgaagc agttcttccc gctccgcaag atccggattc
     6421 cggtacagca tcccggtcag tcgcatggca gcccccttca tccgggttaa tgcctcgccc
     6481 gggatcagtt caccgtcctc acgaatcacc ttatcccggc tgccctgaat gtaggccagc
     6541 agcacggcgg tagcctgacg aaccttgtcc atcagcatgt catcatccgc gtcatggtcg
     6601 acacgcagat gtgccttgat ctcttccagt gtcagtaatg ccgtcatttt ccgcctccag
     6661 catcccgccc acgttttgca gccagggtcc aggctaatga atgagcttct ccgggtttat
     6721 caccggtcat actgttgcag tgccacagcg agccccccca tgtcaccgta tcgccggggt
     6781 ggtaggtttc accggctctg aacacaccgc ggtagagcat caccggcagg gaaaatgttt
     6841 tttccgtacg ctggccactg ctctgccgga ccaccacaga gaacaaccgt tcacccgtca
     6901 tgctgacgtc aatatccgcc accccgtcaa ccaggcattc ccatccccgc atcccgtgcg
     6961 ttttttcata cgcccgccag agtccgccct ggtgtgtggc atacgtgccc cggggaaagg
     7021 atttttgatc gtcaatggcg gggtgtattt ccagtgccgt ggcatcacgc ccgtcctgcg
     7081 gagccggcag ggcactcacc gcatccagaa ccgccttctg cagtacttcc ggatcgtagt
     7141 cacgaccatc
//
LOCUS       DAZFRN010000213         7122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFRN010000213 DAZFRN010000000
VERSION     DAZFRN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.215"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..673)
                     /locus_tag="ACHKUB_004618"
     CDS             complement(<1..673)
                     /locus_tag="ACHKUB_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6657533.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQH"
     gene            complement(769..957)
                     /locus_tag="ACHKUB_004619"
     CDS             complement(769..957)
                     /locus_tag="ACHKUB_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6657534.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKFSGE
                     CLPLKKVSLYLNNETPAG"
     gene            complement(954..1142)
                     /gene="dicB"
                     /locus_tag="ACHKUB_004620"
     CDS             complement(954..1142)
                     /gene="dicB"
                     /locus_tag="ACHKUB_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6657535.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            complement(1675..2049)
                     /locus_tag="ACHKUB_004621"
     CDS             complement(1675..2049)
                     /locus_tag="ACHKUB_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657536.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(2072..2290)
                     /gene="ydfC"
                     /locus_tag="ACHKUB_004622"
     CDS             complement(2072..2290)
                     /gene="ydfC"
                     /locus_tag="ACHKUB_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO6657537.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(2320..2448)
                     /gene="ydfB"
                     /locus_tag="ACHKUB_004623"
     CDS             complement(2320..2448)
                     /gene="ydfB"
                     /locus_tag="ACHKUB_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO6657538.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(2450..2605)
                     /locus_tag="ACHKUB_004624"
     CDS             complement(2450..2605)
                     /locus_tag="ACHKUB_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6657539.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2878..3594)
                     /locus_tag="ACHKUB_004625"
     CDS             complement(2878..3594)
                     /locus_tag="ACHKUB_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6657540.1"
                     /translation="MSTIATSLGGNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            3644..3859
                     /locus_tag="ACHKUB_004626"
     CDS             3644..3859
                     /locus_tag="ACHKUB_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6657541.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            3856..4281
                     /locus_tag="ACHKUB_004627"
     CDS             3856..4281
                     /locus_tag="ACHKUB_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6657542.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            4304..5266
                     /locus_tag="ACHKUB_004628"
     CDS             4304..5266
                     /locus_tag="ACHKUB_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6657543.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGPMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            5307..5729
                     /locus_tag="ACHKUB_004629"
     CDS             5307..5729
                     /locus_tag="ACHKUB_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6657544.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            5726..5980
                     /locus_tag="ACHKUB_004630"
     CDS             5726..5980
                     /locus_tag="ACHKUB_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657545.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            5973..6284
                     /locus_tag="ACHKUB_004631"
     CDS             5973..6284
                     /locus_tag="ACHKUB_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657546.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGNGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            6417..6938
                     /locus_tag="ACHKUB_004632"
     CDS             6417..6938
                     /locus_tag="ACHKUB_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6657547.1"
                     /translation="MGITKERLLEIANLSDWALSDERIVSPHAYESVTSIEITTMARM
                     LLGYFKIENKKQMDSNVDICDILDDWGAWVVAGNSSIDWQEIADKYKNVVPHGKKSRR
                     QCSNDEGRIIDISILMLERYKQQEYELIVAHFVIGLSLRAIAKQQGCSDGTIRKRLQK
                     ALGFLTGYIAITS"
     gene            complement(6935..>7122)
                     /locus_tag="ACHKUB_004633"
     CDS             complement(6935..>7122)
                     /locus_tag="ACHKUB_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657548.1"
                     /translation="VKFNEWKEFFSTTVSDAEIEAEGSPLRFKSITKHPATALPYHVR
                     HMGVSLYHYTGKKTADS"
BASE COUNT         1916 a         1508 c         1737 g         1961 t
ORIGIN      
        1 tgtgctgttg attggttctg gcgcgggcga gatcaacaac agacgtgtat tttccggttt
       61 ccttgcgttc accttcgcga cgttttttcc agatgcgcat ctctgcctga atttcgggcc
      121 atttggcacc aggcttacat ttatgcttaa cccacccgat ggcatgcagc ttaagctccg
      181 gatacatggc gttaacttct ggcattttca tcaacgcttc aacgatatgt ccgtcgaatg
      241 ttgccatgtc ttcctgcaac aattcctgtg cgctaataac catatcaacg gtgatgtttt
      301 cacatgtgtc gaacttaacc atgacagcgt tctgtacttc aggggccagc ttgtcaaaag
      361 tgacgttcat cggatcggat tcagtctcaa ccgggacaaa ggaagcagac gcctcatccc
      421 agcggttttc ctgcatatat tcagcatccc aggaatcgag ggcagggcgg ggtatgccgg
      481 gtttatcctc acagacaata aatttataag cgcagtcctg agctgcaggg aattgctcca
      541 gaaattgcca atgaaatttt gcgcgtgcgc gacgctcatc accagcttca atggcagtgg
      601 ccaccgcaac agcgctatct tcttttatgg cctgttcatc aggaatagca gcgcaaataa
      661 agaccttact cattttgttt taacctcatt acagatttca gggtgaacga atccctgcca
      721 ttgctggcat ttttaatccg ttggtatggt gttaatatgg ctggagggtt atccagccgg
      781 tgtttcgtta ttcaggtaca gcgatacttt ttttagcggg aggcattcac cagaaaattt
      841 ttgctcgtct cttgcctgga ggcaggattc tttacttgca taaattccgg taatcacatt
      901 ctgtgattca cccgttataa gaaaaaccgt catcatcagt gcaaatgctg aagtcattga
      961 cgttctccga aaataccaag ttcaagaaga gcaattcggg aaagtatgga attatcattg
     1021 agcagataag gctcatattt cctcatatta atggcatctt cagtaaactc ccggttactg
     1081 agcagaacac caatatcaaa acaaccttca gacgtattaa cgtttggtaa taacgtttcc
     1141 attatcgcgt cctcaacaat gaattttgtg atgcagtgcc tggtgcctcc aggtgacgtt
     1201 aaccagttaa caattaacgc cggataaaga gaatccaccc ataacactgt ttttggtttt
     1261 aactgttccg cgtgcgctca gccgcattca ccacatcaca aaattcactt taaaaagggc
     1321 ggcagagcag tcacggagta aaactgatac cgccaaaagt caccagaaaa ttgataacag
     1381 agggcgttgc agcggagttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc
     1441 aacgggggaa ggaataaccc cgccatactt accgccgcgc catttcgcgg attgccacaa
     1501 ccggaagcgc acgatcgaat taaatttaac gacgacctat acagagagac taacttctcc
     1561 gggcgctttc gtgttatgcc ctgacttttc agggatatat ccttttcagt aaactgtcag
     1621 tgccggattc ttatccgtgt ccggcgcacg cactctacct cacctgtgaa taaattaatg
     1681 attaattgat attttgttgt ttgattcaac tttcccatcg gatgtgtgat gctttaaatc
     1741 acaggaatta atactgcttg ctgtaaaatg attttcaagg ggagctattc gaatcccttt
     1801 ctttttcatt aacaagccaa atcctttatt aatgatgtcc attaattcca gaaagtattt
     1861 ttcatgtaaa tcctggttat cagagagctg cttctcttcg tacagcccga taaaggctcg
     1921 gcgtacgtta ccagatatat tgtcgatggt ttctttttct acggtactca ggtcaagagt
     1981 cgccagttga gagcgaacca cattcgctgc catttcctgg aatggcattg gtaaatcttt
     2041 aaattccatt attagcctcg ttggttagct attaacgcgg gtatgtaatc attctggcaa
     2101 tgcttaatgc cgctgctttt tccagcctgg tgatatcctg ctccagagcg gacagatttt
     2161 cagcctgctt agccctggct tcattggccc atttcaggtc ctgcgcagcc ttaattttct
     2221 ggtgcatcca ctcataaagt tcatcatcgg tatagtctgg cgcgatgatg acgggttctc
     2281 gtttctgcat actgattcct cgcggtgctg tttcgcttat cagccgttag attttgccga
     2341 actggaaagc gcctgtttaa actcactgaa gctgagggct tcttcgcctt cggcaaggcc
     2401 ttcgaagtat tcttcgtaag ccttttccat gattgtgttg aaatccatat cactcacctg
     2461 agtttctttc cagccagcga cgggcaccat tttcggtttt aaacgttttg cttttggtat
     2521 acgtcatcgc ggtgaacgta ccgtcctggt tggggaacac gccacatacc agagattcgc
     2581 tgttgccaag atcgatagta tccatgctga cctcatttcc ccttaacgcc ggggtagcgg
     2641 aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc ttacgcctcg
     2701 ggctggctac ttaacccctg accactgccg ggtaactcga agtatttccc tgcgttctgt
     2761 ggggcggggt gggttggtat gttgttaagg taacaagagt tacctttcaa gtcaatacaa
     2821 tgttgcaaaa ggtacatttg agggcataaa aaacccgcaa tgaatgcggg ttctgactca
     2881 gtctaagtat tgatgtattt gtgaaacttt acctttaatg gtgtaaccac cattcagttc
     2941 gatgggtttg taaagcggat tcagtgacaa cagatagatg tttggtccgt caatcgcaac
     3001 tttttttagt gttacgtttg gcgttccttc caattggatt aagattattt ttcccaccag
     3061 ttctctaatg ttacttgagc atggtgtgat cagcacggta gatccgtcgg ggatggttgg
     3121 gaggccgtta gagtttgtca tcgcatctcc ctcaacatgc aataaaaaag agttttcagc
     3181 ggtttttgtc atgacatcaa cccagttctt aataccagga atcttggtta ctggacaact
     3241 catatcccaa taaccagcct gttcccacgt taaaacgggc aaccgggcga tgttgtcact
     3301 aatgtaaggg tactgattca gacgcagatc atcggtttta tcgtgaccgt cctttccata
     3361 aagaatccat tcaggagatt tggaaagcaa ttttgacagt agatacaaat tctcaccgtc
     3421 aggttttgaa gagccatttt cccattttgt tacggataca cgagatatgc cgattgcttt
     3481 cgcaacctgc tgttgggtta atccaacgtc ttttcgacga ttccgaatac gttcgctgat
     3541 agtgtttttc atgtaaccaa tgttaccacc aagtgatgtt gctatggttg acattgttgt
     3601 gtaactattg ttaccattct gctcgaaata acaggagagt tttatgttca aagatgatgt
     3661 tctgcgctat ttcaaaaaaa agcgactagt agctgaggct cttggaattt cacatgtggc
     3721 tgttgtgcgg tggaaagcag ttattcccaa acttcgcgca atggaactgg atgaaattac
     3781 taacggtgaa ttgaaataca acccagaact ttacaagaag caggatagca cctcgaacga
     3841 aggaaagaat gattcatgaa aatcaagcat gaacacatcc gcatggcgat gaatgtttgg
     3901 gcgcatccgg acggcgaaaa agtgccggct gcgaaaatta ccaaagcgta tttcgagctg
     3961 ggaatgacgt tcccggaact gtatgacgac agccatccgg aagccctggc ccgtaatacc
     4021 cagaaaattt tccgttggct ggataaagac acccctgatg ctgttgaaaa aatgcaggct
     4081 ctgttaccgg cgatcgaaaa ggcgatgccg cctttgctgg tggcccgtat gcgcagccac
     4141 agttctgaat attaccgtga gatcgtcgaa cggagggatc ggctggtgaa ggatgtcgat
     4201 gattttgttg cgtcagcggt tgttttgtat gaccagatga atcgcggcgg cccggcaggg
     4261 aatgctgtgg tgatgcacta aaagcacggt gttcgggggt tttatgagca gcaagcttca
     4321 tggtcttgtc tgggaagggt gcgccttcac cggcatgatc ttatccaggg tggcggttat
     4381 ggcccgtctt gcagactaca gcaatgacga gggcgtgtca tggcctgcca ttgaaactat
     4441 ccggcgtcag atcggtgcaa gaagtgaatc cacagtgaaa tcggctattg cagaactggc
     4501 gaaagagggc tggctgacga aggaagagcg taaggtcggt gggcgtaatg taagcaatat
     4561 ctatcggctt aatgtggaaa aactcgaagc agctgcggcg gcggcgcgtg agtcatataa
     4621 accgaaaaga aaaattagcc cggcaaaaaa tgacccgtta acagttgacc cgtcaaatat
     4681 tgacccctca acggttgacc cgtcaaattt tgatggatca actgttgata aaaaactgcc
     4741 gattaggggg ccgatgattg accccgatcc gtcagtatta aaacctgatc cgtcagataa
     4801 aagatcttct tgtccggacg cttcgcaacc ggacccgcag acggctgaac aggatttttt
     4861 aacccgacac cctgacgcgg ttgtgttcag tgcgaaaaaa cgccagtggg gaagtcagga
     4921 agatttggtg tgcgcacagt ggatctgggg acgaatcgtg agtctttacg agcaggcggc
     4981 cagctatgat ggcgagatca ctagaccgaa agaacccaac tggacagcat gggccaatga
     5041 cgttcgcaca atgcggatgc tggatggcag aactcacaga caaatttgtg aaatgtttgg
     5101 gcgtctccag cgggattcgt tctgggtaaa aaacatcatg agtccggcaa aactccggga
     5161 aaaatgggat gaactggtta tccgcctggg gcgttcgcct gcgcagcgtt gcgtgaatca
     5221 catttctgaa ccggacactg aaattccgcc gggcttcagg gggtaagtgt taatttctgg
     5281 tcatgaggta attttcagga gggcttgtgg caaaagtttt tacacaagaa gagcgggaaa
     5341 aaattaaagg gcaggttgtt gaactcgtac gccagagtgg gcgcgagacg ttacgacaac
     5401 tggaaactaa aactggggca acaagatatc tgatgagcgt tctggccaga gagctggttg
     5461 ccagtggcga tgtatacaac tctggttacg ggttattccc gtctgaacag gcgcgtaagg
     5521 actggcaaaa tgcccgtaaa aagctctcaa gggcaaagcc gaagaaacca tctgcggttg
     5581 atccggacct tatctggtca ttaccagatg gcgaaatacg ccgctacgac aggcgtctga
     5641 acataatctg tctcgagtgc cggaagagcg aagttatgca gcgcgtactg gcgttttatc
     5701 aggggaattt tgaggaggtg gtgcggtgag tgaatcaaaa tgccaggtta atggcaacaa
     5761 gatagaacca tgtgcagcac tggcaaagtc ccttgagcat gatgctgaat acacgatgcg
     5821 aaaaggtctg ctgatataca aaatctggaa tgagagttta actcgcggtc ctgattttgt
     5881 gatgttgcgt tccggtgaat tttctaaatt accagttcgg gtttcatttt gtccgttctg
     5941 tggtgaaagt ctgaaaacgt gggagaacag aaatgaatga aatcaaagaa ataccagtag
     6001 tacgtgatga atatggctgc tggacgcatc ctgaatatga aaaattctgt gacggtcggg
     6061 aatatatttc aacggaagag tttaacgcct ggatggagga aaataatctt caatacgtcc
     6121 tctgcttcag agatgaagga tgtgctgacc ttgatgcgtg tgatgctgat atttctgcat
     6181 gggaaccgga acgaccagag ggcaatggat ggtttattgg ttcaatacat gacaccgaag
     6241 atggcccggt ttgtgtatgg ctgagaaata aggccgaagc ataaaggcta taaaccgact
     6301 aacaactaaa tactgaagat ttaaatcaga aacgattttt attaaatcct taaccggagg
     6361 gattcctgca ccctcaaatc atcaggaggc cgcccgaaag ggcggtggag ataataatgg
     6421 gaataactaa agaacgattg ttggaaatag caaaccttag tgattgggca ttaagtgatg
     6481 agagaattgt ttctcctcat gcttatgagt cagttacaag tatagaaata acaacaatgg
     6541 ctagaatgct gcttggttat ttcaaaatag aaaataaaaa acagatggat agtaatgttg
     6601 atatatgtga tattttagac gattgggggg cttgggttgt ggctggtaat agttctattg
     6661 attggcagga aatagctgat aaatataaaa atgttgttcc tcatggtaaa aaatcacgtc
     6721 gtcagtgcag caatgatgaa gggcgaatta ttgacattag tatccttatg ttagaaagat
     6781 ataagcaaca agaatatgag ttaattgttg ctcatttcgt gattggttta tctcttcgtg
     6841 ctattgcaaa gcaacaagga tgttcagatg gaacaattcg taaaagattg caaaaagcct
     6901 taggtttttt gactggatat atagcaatta ccagttaaga gtcagcggtt tttttaccag
     6961 tgtaatggta taaggatact cccatgtgtc ttacatgata tggaagcgct gttgctggat
     7021 gctttgtgat actcttaaag cggaggggag agccttccgc ttcaatttct gcgtccgaaa
     7081 cggtcgtaga gaaaaattct ttccattcat tgaattttac tg
//
LOCUS       DAZFRN010000214         7025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFRN010000214 DAZFRN010000000
VERSION     DAZFRN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.216"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            556..1545
                     /gene="araF"
                     /locus_tag="ACHKUB_004634"
     CDS             556..1545
                     /gene="araF"
                     /locus_tag="ACHKUB_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO6657549.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1615..3117
                     /gene="araG"
                     /locus_tag="ACHKUB_004635"
     CDS             1615..3117
                     /gene="araG"
                     /locus_tag="ACHKUB_004635"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO6657550.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVS"
     gene            3144..4130
                     /gene="araH"
                     /locus_tag="ACHKUB_004636"
     CDS             3144..4130
                     /gene="araH"
                     /locus_tag="ACHKUB_004636"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO6657551.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4297..5097
                     /gene="otsB"
                     /locus_tag="ACHKUB_004637"
     CDS             4297..5097
                     /gene="otsB"
                     /locus_tag="ACHKUB_004637"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO6657552.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5072..6496
                     /gene="otsA"
                     /locus_tag="ACHKUB_004638"
     CDS             5072..6496
                     /gene="otsA"
                     /locus_tag="ACHKUB_004638"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO6657553.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6503..6931)
                     /gene="uspC"
                     /locus_tag="ACHKUB_004639"
     CDS             complement(6503..6931)
                     /gene="uspC"
                     /locus_tag="ACHKUB_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO6657554.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1774 a         1630 c         1824 g         1797 t
ORIGIN      
        1 gttttttgta tcttgttgat attaaacgat gaatttgaat tcatgatgaa gtgatgattt
       61 tataaaacgt tttttcattt ttgcgagatg gctctcatta tacgtgttct gttaattaat
      121 gagcacggtg ataattaatg gcgaaatgaa tgacgcgcat tttccacatc tttgagttgt
      181 ggttattaca ccatttcaaa aactcaacgc caggtaatgc ggcctattga ctggttaaaa
      241 agaagacatc ccgcatgggt accaaagaca acaaggattt ccaggctaat cttatggatt
      301 aatctgctgt gcattcgaca atttgtctga caaattggct ttcccttatg tcttttcccg
      361 ctaaatttat gcacgttctc actgtaattc tgcgatgtga tattgctctc ctatggagaa
      421 ttaatttctc gctaaaacta tgtcaacaca gtcacttatc ttttagttaa aaggtaatgc
      481 tttgttttcc gattaattta acgaatgtca ttcgtttttg ccctacacaa aacgacacta
      541 aagctggaga gaaccatgca caaatttact aaagccctag cagccattgg tctggcagcc
      601 gttatgtcac aatccgctat ggcggagaac ctgaagctcg gtttcctggt gaagcaaccg
      661 gaagagccgt ggttccagac cgaatggaag tttgccgata aagccgggaa ggatttaggg
      721 tttgaggtta ttaagattgc cgtgccggat ggcgaaaaaa cattgaacgc gatcgacagc
      781 ctggctgcca gtggcgcaaa aggtttcgtt atttgtactc cggaccccaa actcggctct
      841 gccatcgtcg cgaaagcgcg tggctacgat atgaaagtca ttgccgtgga tgaccagttt
      901 gttaacgcca aaggtaagcc aatggatacc gttccgctgg tgatgatggc ggcgactaaa
      961 attggcgaac gtcagggtca ggaactgtat aaagagatgc agaaacgtgg ctgggatgtc
     1021 aaagaaagcg cggtgatggc gattaccgcc aacgaactgg atacggcacg tcgccgtaca
     1081 acgggttcta tggatgcgct gaaagcggcc ggattcccgg aaaaacaaat ttatcaggta
     1141 cctaccaaat ctaacgacat cccgggggca tttgacgctg ccaactcaat gctggttcaa
     1201 catccggaag ttaaacattg gctgatcgtc ggtatgaacg acagcaccgt gctgggcggc
     1261 gtacgcgcga cggaaggtca gggctttaaa gctgccgata tcatcggcat tggcattaac
     1321 ggtgtggatg cggtgagcga actgtctaaa gcacaggcaa ccggcttcta cggttccctg
     1381 ctgccaagcc cggacgtaca tggctataaa tccagcgaaa tgctttacaa ctgggtagca
     1441 aaagacgttg aaccgccaaa atttaccgaa gttaccgacg tggtgctgat cacgcgtgac
     1501 aactttaaag aagaactgga gaaaaaaggt ttaggcggta agtaatttgc cggaaaaatt
     1561 cccctctgca tgatgcagag ggggtgtgaa cgaccagtga ttcacggaga cgttatgcaa
     1621 cagtctaccc cgtatctctc atttcgcggc atcggtaaaa cgtttcccgg cgttaaggcg
     1681 ctgacggata ttagttttga ctgctatgcc ggtcaggttc atgcgttgat gggtgaaaat
     1741 ggcgcaggaa aatcaactct cttaaaaatc ctcagcggca actatgcgcc aaccacgggt
     1801 tctgtagtga ttaatgggca ggaaatgtcc ttttccgaca cgaccgcagc acttaatgcg
     1861 ggtgtggcga ttatttacca ggaactgcat ctcgtgccgg aaatgaccgt cgcggaaaac
     1921 atctatctcg gccagctgcc gcataaaggc ggcattgtga atcgctcatt gctgaattat
     1981 gaggcgggtt tacaacttaa acatcttggt atggatattg acccggacac gccgctgaaa
     2041 tatctctcca ttggtcagtg gcagatggtt gaaatcgcca aggcgctggc gcgtaacgct
     2101 aaaattatcg cctttgatga gccaaccagc tccctttctg cccgcgaaat cgacaatctt
     2161 ttccgcgtta ttcgtgaact gcgaaaagag gggcgggtga tcttatacgt ttctcaccgt
     2221 atggaagaaa tatttgccct cagcgatgcc atcactgtct ttaaagatgg acgttatgtc
     2281 aaaaccttta ccgatatgca gcaggttgac cacgacgcgc tggtgcaggc gatggtcggg
     2341 cgcgacattg gcgatatcta cggctggcaa cctcgtagtt atggcgagga gcgcctgcgt
     2401 cttgatgctg tgaaagcacc aggcgtgcgt acgccaataa gtctggcggt tcgcagtggt
     2461 gaaattgttg ggctgtttgg tctggtagga gcggggcgta gcgaattaat gaaaggcttg
     2521 tttggcggga cgcaaatcac cgccggtcag gtttatatcg accaacagcc gatcgatatt
     2581 cgtaaaccga gccacgccat tgccgcaggc atgatgctct gcccggaaga tcgcaaagcg
     2641 gaaggcatta ttcccgtgca ctccgttcgc gacaatatca acatcagtgc cagacgtaaa
     2701 catgtgctcg gcggttgtgt aatcaacaac ggttgggaag aaaacaatgc cgatcaccac
     2761 attcgttcgc tcaacatcaa aacgccgggc gctgagcaac tgatcatgaa tctctcaggc
     2821 ggaaatcagc aaaaagccat tctgggccgc tggttatcgg aagagatgaa ggtcattttg
     2881 ctggatgaac ctacgcgcgg cattgatgtt ggtgctaagc atgaaattta caacgtgatt
     2941 tatgcgctgg cggcgcaggg tgtggcggtg ctgtttgcct ccagcgactt acctgaagtc
     3001 ctcggcgttg ccgaccggat tgtggtgatg cgggaaggtg aaatcgccgg tgaattgtta
     3061 cacgagcagg cagatgagcg tcaggcactg agccttgcga tgcctaaagt cagctaggca
     3121 gttgcctgag taaggagagt atgatgtctt ctgtttctac atcggggtct ggcgcaccta
     3181 agtcgtcatt cagcttcggg cgtatctggg atcagtacgg catgctggtg gtgtttgcgg
     3241 tgctctttat cgcctgtgcc atttttgtcc caaattttgc caccttcatt aatatgaaag
     3301 ggttgggcct ggcaatttcc atgtcgggga tggtggcgtg tggcatgttg ttctgccttg
     3361 cttccggtga ctttgacctt tctgtcgcct ccgtaattgc ctgtgcgggt gtcaccacgg
     3421 cggtggttat caacctgact gaaagcctgt ggattggcgt ggcagcgggg ttgctgcttg
     3481 gcattctctg tggcctggtc aatggctttg ttatcgccaa actgaaaata aatgctctga
     3541 tcacaacact ggcaacgatg cagattgttc gaggtctggc gtacatcatt tcagacggta
     3601 aagcggtcgg tatcgaagat gaaagcttct ttgcccttgg ttacgctaac tggttcggtc
     3661 tgcctgcgcc aatctggctc accgtcgcgt gtctgattat ctttggtttg ttgctgaata
     3721 aaaccacctt tggtcgtaac accctggcga ttggcgggaa cgaagaggct gcgcgtctgg
     3781 cgggtgtacc ggttgttcgc accaaaatta ttatctttgt tctctctggc ctggtatctg
     3841 cgatagccgg aattattctg gcttcacgta tgactagtgg gcagccaatg acgtcgattg
     3901 gttatgagct tattgttatc tccgcctgcg ttttaggtgg cgtttctctg aaaggtggca
     3961 tcggaaaaat ctcatatgtg gtggcgggta tcttaatttt aggcaccgtg gaaaacgcca
     4021 tgaacctgct taatatttct cctttcgcgc agtacgtggt tcgcggctta atcctgctgg
     4081 cagcggtgat cttcgaccgt tacaagcaaa aagcgaaacg cactgtctga tgcttttttc
     4141 cacaacaatt taacgttttt tcccaccaca gccagccgcc acaacggttg gctgttcttc
     4201 attgcaaatg gcgacccccg tcacactgtc tatacttaca tgtctgtaaa gcgcgttctg
     4261 cgcaaaacaa taagaaaaga gaaggaggag agccgggtga cagaaccgtt aaccgaaacc
     4321 cctgaactat ccgcgaaata tgcctggttt tttgatcttg atggaacgct ggcggaaatc
     4381 aaaccgcatc ccgatcaggt cgtcgtgcct gacaatattc tgcaaggact acagctactg
     4441 gcaaccgcaa gtgatggtgc attggcattg atatcagggc gctcaatggt ggagcttgac
     4501 gcactggcaa aaccttatcg cttcccgtta gcgggcgtgc atggggcgga gcgccgtgac
     4561 atcaatggta aaacacatat cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg
     4621 caactgcata cagtcatcgc tcagtatccc ggcgcggagc tggaggcgaa agggatggct
     4681 tttgcgctgc attatcgtca ggctccgcag catgaagacg cattaatgac attagcgcaa
     4741 cgtattactc agatctggcc acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc
     4801 aaaccgagag gtaccagtaa aggtgaggca attgcagctt ttatgcagga agctcccttt
     4861 atcgggcgaa cgcccgtatt tctgggcgat gatttaaccg atgaatctgg cttcgcagtc
     4921 gttaaccgac tgggcggaat gtcagtaaaa attggcacag gtgcaactca ggcatcatgg
     4981 cgactggcgg gtgtgccgga tgtctggagc tggcttgaaa tgataaccac cgcattacaa
     5041 caaaaaagag aaaataacag gagtgatgac tatgagtcgt ttagtcgtag tatctaaccg
     5101 gattgcacca ccagacgagc acgccgccag tgccggtggc cttgccgttg gcatactggg
     5161 ggcactgaaa gccgcaggcg gactgtggtt tggctggagt ggtgaaacag ggaatgagga
     5221 tcagccgcta aaaaaggtga aaaaaggtaa cattacgtgg gcctctttta acctcagcga
     5281 acaggacctt gacgaatact acaaccaatt ctccaatgcc gttctctggc ccgcttttca
     5341 ttatcggctc gatctggtgc aatttcagcg tcctgcctgg gacggctatc tacgcgtaaa
     5401 tgcgttgctg gcagataaat tactgccgct gttgcaagac gatgacatta tctggatcca
     5461 cgattatcac ctgttgccat ttgcgcatga attacgcaaa cggggagtga ataatcgcat
     5521 tggtttcttt ctgcatattc ctttcccgac accggaaatc ttcaacgcgc tgccgacata
     5581 tgacaccttg cttgaacagc tttgtgatta tgatttgctg ggtttccaga cagaaaacga
     5641 tcgtctggcg ttcctggatt gtctttctaa cctgacccgc gtcacgacac gtagcgcaaa
     5701 aagccataca gcctggggca aagcatttcg aacagaagtc tacccgatcg gcattgaacc
     5761 gaaagaaata gccaaacagg ctgccgggcc actgccgcca aaactggcgc aacttaaagc
     5821 ggagctgaaa aacgtacaaa atatcttttc tgtcgaacgg ctggattatt ccaaaggttt
     5881 gccagagcgt tttctcgcct atgaagcgtt gctggaaaaa tatccgcagc atcatggtaa
     5941 aattcgttat acccagattg caccaacgtc gcgtggtgat gtgcaagcct atcaggatat
     6001 tcgtcatcag cttgaaaatg aagctggacg aattaatggt aaatacgggc aattaggctg
     6061 gacgccgctt tattatttga atcagcattt tgaccgtaaa ttactgatga aaatattccg
     6121 ctactctgac gtgggcttag tgacgccact gcgtgacggg atgaacctgg tagcaaaaga
     6181 gtatgttgct gctcaggacc cagccaaccc gggcgttctt gttctttcgc aatttgcggg
     6241 agcggcaaac gagttaacgt cggcgttaat tgttaacccc tacgatcgtg acgaagttgc
     6301 agctgcgctg gatcgtgcat tgactatgtc gctggcggaa cgtatttccc gtcatgcaga
     6361 aatgctggac gttatcgtga aaaacgatat taaccactgg caggagtgct tcattagcga
     6421 cctaaagcag atagttccgc gaagcgcgga aagccagcag cgcgataaag ttgctacctt
     6481 tccaaagctt gcgtaggagc ggttaatcac ccgtaagtgg aactaacagc acatcgacct
     6541 cacttgaggc aatcactctt ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt
     6601 gattaccgca aatcaccaaa tcgaaatgat gtttgtgaca tacttccaga atatgttcgc
     6661 ttaactcacc gtaggcaata aacgtcttgt caacgggata ccccgcatcc tgaattaact
     6721 tatcaagaaa gctttgcgtt tcttcctgca tcacgctgcg taaatcttca agcatcggcg
     6781 cagctaattg attgtacatt tccgggtcgg aagcgagagt aattaaactg atgtgtccct
     6841 ttactggcct ggcgatagat accgcttttg ccagcagttg ctgactttcc ggtgtaaccg
     6901 caacagcgac aagaatattg ctatagctca taacctgttc cttattctgt gaacttcagg
     6961 tgacattaaa gctttactcc taatggagaa acgacgcaat cccaactcag tcaaacaggt
     7021 aaata
//
LOCUS       DAZFRN010000215         6939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFRN010000215 DAZFRN010000000
VERSION     DAZFRN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.217"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(91..1740)
                     /gene="pgi"
                     /locus_tag="ACHKUB_004640"
     CDS             complement(91..1740)
                     /gene="pgi"
                     /locus_tag="ACHKUB_004640"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO6657555.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      1977..2173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2265..3614
                     /gene="lysC"
                     /locus_tag="ACHKUB_004641"
     CDS             2265..3614
                     /gene="lysC"
                     /locus_tag="ACHKUB_004641"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO6657556.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3759..4685
                     /locus_tag="ACHKUB_004642"
     CDS             3759..4685
                     /locus_tag="ACHKUB_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO6657557.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNHIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLADKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4721..5512)
                     /locus_tag="ACHKUB_004643"
     CDS             complement(4721..5512)
                     /locus_tag="ACHKUB_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO6657558.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5518..6645)
                     /locus_tag="ACHKUB_004644"
     CDS             complement(5518..6645)
                     /locus_tag="ACHKUB_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO6657559.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1711 a         1809 c         1660 g         1759 t
ORIGIN      
        1 cggtatgatt tccgttaaat cacagacaag gggatttatc tgataaaaca atgccgacat
       61 aacgtcggca ttgttattaa ggtttttaaa ttaaccgcgc cacgctttat agcggttaat
      121 cagaccattg gtcgagctat cgtggctgct gatttcttta tcatctttca gctctggcag
      181 aatacggttt gccagttgtt tacccagctc cacgccccac tggtcgaagg tgaagatgtt
      241 caggatcacg ccctgagtaa agattttgtg ctcatacagc gcaatcaacg cacccaggct
      301 gaacggagtg atttcacgca gcaggatgga gttggtcggg cggttacctt cgaacacttt
      361 gaacggcacc acgtagtcaa gcgttgccgg atctttaccc tgatcacgat attcctgctc
      421 aaccacttcg cgggatttac caaacgccag cgcttcggtc tgggcgaaga agttagacag
      481 cagtttctgg tgatgatcag agagcgggtt atgggtgata gccggagcga tgaaatcgca
      541 cggtaccatt ttggttccct ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc
      601 tggttcaccc cagataatcg ggccagtctg gtaatccaca acgttaccgt tacggtcaac
      661 atacttaccg ttggactcca tattgccctg ctggaagtac gccgcgaaac ggtgcatata
      721 ctggtcatac ggcagaatcg cctcagtttc cgcaccaaag aaattgttgt accagatgcc
      781 aatcagcgcc agcagtacag gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc
      841 catcgcgtgt gcgccagaaa gcagttcaac gaagttatca aagccgatgg agagaacaat
      901 cgacaggcca atcgctgacc acaaagagta acggccgccg acccagtccc agaactcgaa
      961 catgttggca gtatcaatac caaactcgcc aacggctttg gcattggttg agagcgccgc
     1021 aaagtgtttc gcaacgtgtt tttcatcacc tgccgctttc aggaaccagt cacgcgcgct
     1081 atgggcgttg gtcatggttt cctgagtggt gaaggtttta gatgctacca ggaacagcgt
     1141 ggtttccggg tttacttttt tcagcacttc cgcgatgtga gtcccatcga cgttagaaac
     1201 aaagtgcatg ttcaggtgat ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc
     1261 gaggtcagaa ccgccgatcc cgatgttcac tacgtcagtg attgctttgc cggtataacc
     1321 cttccactca ccggagataa tcgcttccga gaaggttttc atcttctcca gcaccgcgtt
     1381 gacttccggc attacgtctt tgccatcaac caaaatcggg gtattgctac ggttacgcag
     1441 cgctacgtgc agcacagcgc ggttttcagt gcggttgatc ttctcgccag agaacatcga
     1501 cttaatcgcg cccgccagat cgcactcttt cgccagatct tgtaatttcg ccagcgtctc
     1561 ttcagtgatg cggtttttgg agtaatccac cagcatctga tcgccgaagg ttgcggagaa
     1621 cttagaaaaa cgatcgccgt ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc
     1681 atcgaagtgt ttctgtagtg cctgccaggc agcggtctgc gttggattga tgtttttcat
     1741 tagcaatact cttctgattt tgagaattgt gactttggaa gattgtagcg ccagtcacag
     1801 aaaaatgtga tggttttagt gccgttagcg taatgttgag tgtaaaccct tagcgcagtg
     1861 aagcatttat tagctgaact actgaccgcc aggagtggat gaaaaatccg catgaccccg
     1921 tcgttgacaa ccgccccgct caccctttat ttataaatgt actacctgcg ctagcgcagg
     1981 ccagaagagg cgcgttgccc aagtaacggt gttggaggag ccagtcctgt gataacacct
     2041 gagggggtgc atcgccgagg tgattgaacg gctggccacg ttcatcatcg gctacagggg
     2101 ctgaatcccc tgggttgtca ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag
     2161 cacttctggg tgaaaatagt agcgaagtat cgctctgcgc ccacccgtct tccgctcttc
     2221 ccttgtgcca aggctgaaaa tggatcccct gacacgaggt agttatgtct gaaattgttg
     2281 tctccaaatt tggcggtacc agcgtagctg attttgacgc catgaaccgc agcgctgata
     2341 ttgtgctttc tgatgccaac gtgcgtttag tagtcctctc ggcttctgct ggtatcacta
     2401 atctgctggt cgctttagcg gaagggctgg aacctggcga gcgattcgaa aaactcgacg
     2461 ctattcgcaa catccagttt gccattctgg aacgtctgcg ttacccgaac gttatccgtg
     2521 aagagattga acgtctgctg gagaacatta ctgttctggc agaagcggcg gcgctggcaa
     2581 cgtctccggc gctgacagat gagctggtca gccacggcga gctgatgtcg accctgctgt
     2641 ttgtcgaaat cctgcgcgaa cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga
     2701 tgcgtaccaa cgaccgattt ggtcgtgcag agccagatgt agccgcgctg gcggaactgg
     2761 ccgcgctgca gctgctccca cgcctcaatg acggcttagt gatcacccag ggatttatcg
     2821 gtagcgaaaa taaaggtcgt acaacgacgc ttggccgtgg aggcagcgat tatacggcag
     2881 ccttgctggc ggaggcttta cacgcatctc gtgttgatat ctggaccgac gtcccgggca
     2941 tctacaccac cgatccacgc gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg
     3001 ccgaagcggc agagatggca acttttggtg caaaagtact gcatccggca acgttgctac
     3061 ccgcagtacg cagcgatatc ccggtctttg tcggctccag caaagaccca cgcgcaggtg
     3121 gtacgctggt gtgcaataaa actgaaaatc cgccgctgtt ccgcgcgctg gcgcttcgtc
     3181 gcaatcagac tctgctcact ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg
     3241 cggaagtttt cggcatcctc gcgcggcata atatttcggt agacttaatc accacgtcag
     3301 aagtgagcgt ggcattaacc cttgatacca ccggttcaac ctccactggc gatacgttgc
     3361 tgacgcaatc tctgctgatg gagctttccg cactgtgccg ggtggaggtg gaagaaggtc
     3421 tggcgctggt cgcgttgatt ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg
     3481 tattcggcgt actggaaccg ttcaacattc gcatgatttg ttacggcgca tccagccata
     3541 acctgtgctt cctggtgccc ggcgaagatg ccgagcaggt ggtgcagaag ctgcatttta
     3601 atttgtttga gtaatatttc cctactggcg tataacgctc accgattgtt atacgccatc
     3661 ctcactgaaa acatagatta aataatatct gaaaagcaaa atcactcctg tagaacagta
     3721 ttataatcac cgaaaaatat catttaacag gagtgttaat ggctacttta atggaaaaag
     3781 atgcactttt aaacggagcc agtcagtgca tcgcgttttt atcaaatatc attgataatt
     3841 gctcagtttc gtcgcatcag gattcaggcg atgctctcaa gcgattagtc agctatcgcg
     3901 attatcttta ttctaccccc gctgaacttg ttgattttac acaaggtaag attctccttc
     3961 agcaggtaag aactcaatat cagcatgaat tcaacaatac tacgcattca gaaaataaag
     4021 catcttttga ttctatttgg cagcgtctga caaatcatga agtcacaccg caacagcatc
     4081 ccattggttt tgttttaggt gggcaacccg gagcaggtaa atcatctctg attgaattag
     4141 ccaaaagaga gacaaaagat aatataatga ttattaacgg tgatgatttt cgatttctac
     4201 accctgattt taatcatatc taccaaaatt atggggatga ttttgttacc catacagcga
     4261 aattttccgg cgaaacggtc gaacgtgcaa ttgagagagc gatagtcagt aaactcaaca
     4321 ttgttgtcga aggaacattt cgtaatgccg ctacgccgtt acaaacatta aaaaaactca
     4381 aagatgcagg ctaccaaaca gaagtcatga taaaaacaac ctcagcggca ttaagctggg
     4441 aaagcaccaa tgaacggtat aataaggaca aagaggcggg aaatattgcc cgaaaagtgg
     4501 ataaaaacca ccatgatatc gtcaccggtt tgctggcaga aaatgcccgc aaggtgtttg
     4561 cttcaaacct ggcagataag tttgcggtgt attcgcgtga gaagatgata tttagttcac
     4621 aagcggcaac gaatgacgat attgcaacgc ttatacagaa tgaaatcagc gggaatacac
     4681 aataataata agagctgatg gcgtgaaata acgccgggca tcacgcccgg cgcattaccg
     4741 tctggtacat taagctatcc agttccttaa cgtccccttc tctgctatcc ggtaacagcg
     4801 gtggaatttc cccacgcgcc agttccgcac gaataaacgc atgtaacaat ggacggcgcg
     4861 gaccatattc cgcttctcct gcgcgctgtt tacgttccag taattcgtct atttctgcac
     4921 gtaacggcgc atcaagttcg cttcccgcca gtagctcagc aaagcgcatt ggcggtacgc
     4981 cttttcccgc ttcaacccaa cgcacggcca gcagcggacg cagcacgtaa aagtattttt
     5041 tcagccgcac ttcgtcgcct tgtaaataac cgcgaaagtt tttccgcgcc atcgaataat
     5101 aatgccaacg cgcacgcagc ggagaaaacc acttcggcac cattgcctta agcgccgtta
     5161 tcgtttcttc atcttgttga tagaccacag gagaatccag ccattctatc agcgtcggat
     5221 tcgcaccttt caataatccc agcgccttgc gccactccca accggaaaca tctaactcgt
     5281 cgtcgatggg cagctcaata acatcgcgcg gtgactctac tcgcagatac cattccagcg
     5341 ggtgaacata caaaaaccgt acatcgtagt cgctgtcggg cgaggcaaat ccccagccgc
     5401 ggctccctga ttcacaggcg tacagtactt tcacatcata ccgccgttca atttctttta
     5461 actgctgcga taccctttcg cgcatcgcag cactgactcc atttaatggc atgttactca
     5521 ccctttgata ttaaccaacc catgcagaac ctgatccaga ccgccaaata cagagatgtt
     5581 atcgatccgc tcagccactc tctccagcgt ttctagctct tttaaacgca acgctaccgg
     5641 attgttctcc atgactttgg cggtgttcag taacgagcgc gtcgctgccg tttcttcacg
     5701 tcggcgaatc acattcgcct gcgcagattt ctctgcctca accagctgcg cgaggatatt
     5761 tttcatatca ccaggcagga cgatatcttt cacccctaat gaggcaatct ccataccaaa
     5821 tggcaacatg cgcgatttca cctgttcgct gacaacgtca tcaatgacct gtttatcttc
     5881 cagcagttca tccagcgtac gagtacctac cgcttcacgc agggcaaatt gcaattcccg
     5941 atacaaatga tcaatcggct tcgttaactg actaaatgcc agcaacacgt cgctgtagcg
     6001 ccagttagcg gcgaggttaa tccgcagatt gaccttatct ttcgttaaga tttcttgccc
     6061 gctgacttcc agtacctgca agcgggtgtc aaccacttca gcgtcaacca gatggttaat
     6121 tttccagtat gcggtcagcc ccggcggtaa cagcgcctgc gtttcaccat cgattttcag
     6181 cacgcctaca tgccaggccg gaacctgcac cgttagtatt gcatcgcggc ctttaactgc
     6241 ccctttgcgc cgtggttgca ataccgcatt catcacctcc gtttgtacct ggacttgctg
     6301 cgtattcatc cgcaccagtg tcaaatcatc ctctacacgc cagtacaaac ggcgcgttga
     6361 tggcggcagg atctcctgca aaataccgtc catatacaac gccccggctt caatctccga
     6421 caagtctgcc accaggcaat atttctccac ccaatcaggc tgaaagcggc gcaaataatc
     6481 cgccagcacg tcgggaactt cgctgccatc gagcgtaatc agcaagactt ctggcgtatt
     6541 taaccagggt aaaagatgct cgcctgcatg tagcactttg tagtaatcgc cgtttctgga
     6601 gagtaatgcc agctgatctt ttcttacgct tattttttta atcattatct attccttgct
     6661 taacagccgt cgcggaagca gacacgaagc gacaggggaa ttcccttttg cctgttaccc
     6721 ggcatcttta tcggcgacgt tatcggggat cgctgactgc gcaatccctc actttttatt
     6781 taataagtta aaaccagtat tcatttcagg aatgaatgtg ggaatcgaac ccataattgg
     6841 cgtcaaaggc tgacggaaca cgcccggaaa acagcctggc cgttcttggt gtgccgacgg
     6901 ggtttgaccc cctttgtaat tcagtttcaa cgcgtacga
//
LOCUS       DAZFRN010000216         6937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFRN010000216 DAZFRN010000000
VERSION     DAZFRN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.218"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1296
                     /gene="pdeC"
                     /locus_tag="ACHKUB_004645"
     CDS             <1..1296
                     /gene="pdeC"
                     /locus_tag="ACHKUB_004645"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO6657560.1"
                     /translation="YARDNHFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFS
                     GYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPL
                     IHLKDLTVQRNGYLYATVYSTKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVL
                     LLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQI
                     MNPAEFIPLAEKEGMIEQVTDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLI
                     ARINQKTEQYAVRPQQIKFEVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNL
                     HNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNW
                     LRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPARELTRGQ"
     gene            complement(1299..1622)
                     /gene="soxS"
                     /locus_tag="ACHKUB_004646"
     CDS             complement(1299..1622)
                     /gene="soxS"
                     /locus_tag="ACHKUB_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO6657561.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1708..2172
                     /gene="soxR"
                     /locus_tag="ACHKUB_004647"
     CDS             1708..2172
                     /gene="soxR"
                     /locus_tag="ACHKUB_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO6657562.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2718..4067
                     /gene="ghxP"
                     /locus_tag="ACHKUB_004648"
                     /gene_synonym="yjcD"
     CDS             2718..4067
                     /gene="ghxP"
                     /locus_tag="ACHKUB_004648"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO6657563.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4219..5868
                     /locus_tag="ACHKUB_004649"
     CDS             4219..5868
                     /locus_tag="ACHKUB_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO6657564.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6022..>6937)
                     /locus_tag="ACHKUB_004650"
     CDS             complement(6022..>6937)
                     /locus_tag="ACHKUB_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO6657565.1"
                     /translation="INNVAVHNNTIEIPQTVNGGYDFSHLSLKGIVIKDEDLSNSNFA
                     GCRLQNAIFQDCNMYKTNFYYAIMEKILFDDCILDDSNFAQIKMADGTLNACSAMHVQ
                     FYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEH
                     ADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCHF
                     NCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTRQKFFEEDCT
                     SHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1595 a         1581 c         1816 g         1945 t
ORIGIN      
        1 tatgcccgtg ataaccattt tttatgctca tcgctgatag cgcctgtaaa cggctatacg
       61 attgcaccgg ccgattataa gcgtgaacct aacgtttcta tctattatta ccgcgatacg
      121 ccttttttct ctggctataa aatgacctat atgcagcggg gaaattatgt ggcggttatc
      181 aaccctctct tctggagtga agtgatgtct gatgacccga cattgcaatg gggtgtgtat
      241 gatacggtga cgaaaacctt tttctcgtta agcaaagagg cctcggcagc aacgttttct
      301 ccgctgattc atttgaagga tttaaccgta caaagaaatg gctatttata tgcgacagtt
      361 tattcgacaa aacgcccaat tgcagccatt gttgcgactt catatcaacg tcttataacc
      421 catttttata atcatcttat ttttgcgttg cccgccggta ttttggggag tcttgttctg
      481 ctattactct ggctacgtat tcgacaaaac tatttatctc ccaaacgtaa attgcaacgc
      541 gccctcgaaa aacatcaact ttgtctttat taccagccaa taatcgatat caaaacagaa
      601 aaatgtatcg gcgctgaagc gttgttacgt tggcttggtg agcaggggca aataatgaat
      661 ccggcagagt ttattccgct ggcagaaaag gaggggatga tagaacaggt aactgattat
      721 gttattgata atgtcttccg cgatctgggc gattacctgg caacacatgc agatcgctat
      781 gtttctatta acctgtcggc ctccgatttt catacgtcac ggttgatagc gcgaatcaat
      841 cagaaaacag agcaatacgc ggtgcgtccg cagcaaatta aatttgaagt gactgaacat
      901 gcatttcttg atgttgacaa aatgacgccg attattctgg ctttccgcca ggcaggttac
      961 gaagtggcaa ttgatgattt tggtattggc tactctaact tgcataacct taaatcattg
     1021 aatgtcgata ttttgaaaat cgacaaatcg tttgttgaaa cgctgaccac ccacaaaacc
     1081 agtcatttga ttgcggaaca catcatcgag ctggcgcaca gcctggggtt aaaaacgatc
     1141 gctgaaggcg tcgaaactga ggagcaggtt aactggctgc gcaaacgcgg cgtgcgctat
     1201 tgccagggat ggttctttgc gaaggcgatg ccgccgcagg tgtttatgca atggatggag
     1261 caattacccg cgcgggagtt aacgcgcggg caataaaatt acaggcggtg gcgataatcg
     1321 ctgggagtgc gatcaaactg ccgacggaaa acgcgggaga aggtctgctg cgagacataa
     1381 cccaggtcca ttgcgatatc aaaaatcgga cgctcggtgg tgcgcaactc aacggcggcc
     1441 agtaacaggc ggcgttggcg aatgtaatcg ccaagcgtct gatgcgtcac cgtgcggaac
     1501 attcgttgca agtaccactt tgaatagcct gatttttttg cgactacatc aatgttaagc
     1561 ggctggtcaa tatgctcgtc aatccatgcg ataagatcct gaataatttt ctgatgggac
     1621 ataaatctgc ctcttttcag tgttcagttc gttaattcat ctgttgggga gtataattcc
     1681 tcaagttaac ttgaggtaaa gcgatttatg gaaaagaaat taccccgcat taaagcgctg
     1741 ctaacccccg gcgaagtggc gaaacgcagc ggtgtggcgg tatcggcgct gcatttctat
     1801 gaaagtaaag ggttgattac cagtatccgt aacagcggca atcagcggcg atataaacgt
     1861 gatgtgttgc gatatgttgc aattatcaaa attgctcagc gtattggcat tccgctggcg
     1921 accattggtg aagcgtttgg cgtgttgccc gaagggcata cgttaagtgc gaaagagtgg
     1981 aaacagcttt cgtcccaatg gcgagaagag ttggatcggc gcattcatac cttagtggcg
     2041 ctgcgtgacg aactggacgg atgtattggt tgtggctgcc tttcgcgcag tgattgcccg
     2101 ttgcgtaacc cgggcgaccg cttaggagaa gaaggtaccg gcgcacgctt gctggaagat
     2161 gaacaaaact aaagcgccac aagggcgctt tagtttgttt tccggtcttt gtctttctct
     2221 ctatcccgct ggtacacagg agggtttccc ccgacgtcaa cacacctcat tcgagcacgt
     2281 ggtggaggtt ccggttggtg ttgatgcttt aattgtatgt caccgacgtt tcttcgccag
     2341 tgtaaaagta tactttttaa ccgcaatatt tttgtcatct cagacgattt tttatcgcaa
     2401 tcctgaacgg tatacggctc gataacgctg caatcttgcg caccgacgat aacgtttgcg
     2461 catcaattgc ctggtttttc atcgtcaaga caataaaaga gaaaaaagca gcaaacttcg
     2521 gttgaaaaag ccgctatgat cgccggataa tcgtttgctt tttttaccac ccgttttgta
     2581 tgcgcggagc taaacgtttg cttttttgcg acgcagtaaa ttgtcgcaaa cctggagcag
     2641 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2701 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2761 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2821 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2881 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     2941 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3001 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3061 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3121 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3181 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3241 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3301 tcggcctgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3361 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3421 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3481 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3541 ctatccgtgc cgtcgccggc caggcgaacc tgctggataa agatgggcag atcatcgacg
     3601 gtgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgctg
     3661 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccggtt
     3721 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctctctt
     3781 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3841 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3901 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     3961 tggtgattgg tcgtctggtt tccggcgaat ggcgcaagtt gaacatcggt acggtcgtta
     4021 tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat
     4081 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4141 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaac
     4201 atcgcaaaca gggaacgcat ggaaatcttc ttcaccatac tgataatgac cctcgtggtc
     4261 tcgctgtccg gggtggtcac tcgtgtcatg ccctttcaga tcccgcttcc gcttatgcaa
     4321 atcgccatcg gtgcgctact ggcgtggccg acgtttggtt tgcatgtgga gtttgatcct
     4381 gaactctttt tagtcttgtt tatcccgccg ttgctgttcg ctgatggctg gaaaacgccg
     4441 acccgtgaat ttctcgaaca tggtcgagag attttcggcc tcgcgctggc gctggtggtg
     4501 gtcaccgtgg tcggcattgg cttccttatt tactgggtgg tgccgggcat tccgctgatc
     4561 cccgcctttg cgctggcggc ggtactttct ccgaccgatg ctgtggcgct ctccgggatt
     4621 gttggcgaag ggcgcatccc gaaaaaaatc atgggcattt tgcagggcga agcgttgatg
     4681 aatgacgcct ccggcctggt gtcgttgaag tttgccgtgg cagtggcgat ggggacgatg
     4741 atcttcaccg tcggcggtgc gacggtcgaa tttatgaaag tagccattgg tggtattctt
     4801 gccggttttg tggtgagctg gctgtacggt cgctcgctgc gattcctcag ccgctggggc
     4861 ggtgatgaac ccgcgacgca gatcgttctg ctgttcttgc tgccattcgc ttcttatctg
     4921 attgccgaac atattggcgt ttccggcatc ctcgctgcgg ttgccgccgg gatgaccatc
     4981 acccgctccg gtgtgatgcg ccgtgcgccg ctggcaatgc gcctgcgcgc aaacagcacc
     5041 tgggcgatgc tggaatttgt ctttaacggc atggtgttcc tgctgttagg tctgcagctg
     5101 ccgggtattc tggagacgtc gctgatggcg gcagaaatcg accctaacgt cgaaatctgg
     5161 atgctgttta ccgatattat tctgatatat gcggcgctga tgctggtccg tttcggctgg
     5221 ctgtggacga tgaaaaagtt cagcaaccgc ttcctgaaga agaagccgat ggagtttggt
     5281 tcgtggacca cacgagaaat cctgatcgcg tctttcgctg gggtgcgtgg ggcgatcact
     5341 ctggccggtg tgctctctat cccgctgctc ttgccggatg gtaacgtctt cccggcgcgc
     5401 tatgagctgg tgttcctggc ggctggcgtc attctcttct cgctgtttgt cggcgtggtg
     5461 atgttgccta ttctgctaca acacattgaa gtcgcggatc attcgcaaca attgaaagag
     5521 gaacgtattg cgcgagcggc aacggcagaa gtggcgattg tggcgatcca gaaaatggag
     5581 gagcgtctgg cggcggatac cgaagagaat atcgataacc agctgcttac ggaggtcagt
     5641 tctcgcgtca ttggtaacct gcgtcgtcgc gccgatggac gtaacgacgt tgaaagttcc
     5701 gtgcaggaag agaaccttga gcgtcgcttc cgtctggcgg cattgcgttc tgaacgtgct
     5761 gaactgtacc acctgcgcgc cacgcgggag atcagcaacg aaacgctgca aaaattactg
     5821 cacgatctcg atttgcttga agcgttgcta attgaggaaa atcagtaagc cgaaagcgac
     5881 gtaaccaggc ccgaggtaat ctgaaaaagg tgcaatcttt tgcaccttta acaggttagc
     5941 tcgggctaac attcatgatt ctaaaacaaa taagttatct ctcttgtaaa gtcaattcat
     6001 tggctgttct tcgatttaac ctcaatctgc gtgttgcata atatctgcag taatcttcat
     6061 agcgactgca ttaatattat cctcagtttg tgacatgcca gtatgagagg tacaatcttc
     6121 ttcaaagaat ttctgtcgtg ttaccagggt atcactatca atggggatat tttcctcccc
     6181 acggagtatg gaaatgccca ttccctggac gctagcctca tcaaaattaa cccgatagag
     6241 acgggtatta aacatacagg cttttgttaa aatagaacag ttgaaatggc agttgcttaa
     6301 attggcatta ttaaaattaa cattagccat attagagtcg gtacatataa ccatttcgag
     6361 gaatgtattt gttagccacg tacaaaagag tttgctatca attaagttta tattctggag
     6421 gattgctttg ttcagattga cgcgagacag atcagcatgt tcaaagttaa ttaaatcgag
     6481 tttagagaaa ctaaggtcgg ctttaaataa attaacatat ggcgctatta ctttataaag
     6541 atttacctca gccatgtacg ccatataaaa atttgaatag tcaagaaagg tatttttaat
     6601 attggctcta ttcattgctg cattgtagaa ttgaacatgc atagcggagc atgcatttag
     6661 agttccgtcg gccattttta tctgagcgaa atttgagtca tcgagaatac aatcatcaaa
     6721 aagtattttt tccattatgg cgtaataaaa attcgtttta tacatattac agtcctggaa
     6781 aatagcgttt tgtagtctgc aacctgcaaa attcgaattg gataaatctt catctttaat
     6841 cacgatacct ttcaggctaa ggtgtgaaaa atcgtaacca ccatttactg tctgtggaat
     6901 ttcgatagta ttattatgga cggcgacatt atttata
//
LOCUS       DAZFRN010000217         6929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFRN010000217 DAZFRN010000000
VERSION     DAZFRN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.219"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..1622)
                     /locus_tag="ACHKUB_004651"
     CDS             complement(<1..1622)
                     /locus_tag="ACHKUB_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO6657566.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAVEQGLSRQALAAQKAGMSVGQYKAAMRMLPMQFTDVATQLAGGQNPWL
                     ILLQQGGQVKDSFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAF
                     NKTLVLSGNQSGLTADRMLTLSRAGQAAGLTFNQAGESLAALVNAGVRGGEQFDAINQ
                     SVARFASASGVEVDKVAEAFGKLTTDPTLGLMAMARQFRNVTAEQIAYVAQLQRSGDE
                     AGALQAANEAATKGFDDQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPDTAQE
                     MLIKAETAFKKADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQD
                     KNAQQQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQAELNKALRDGKILQADYNTL
                     MASAKKDYESTQKKPKSSGVSVSAGERQEDRAHAALLALETELRTLEKHSGANEKISQ
                     QRRDLWKAESQYAVLKEAATKRQLSGQEKSLLA"
     gene            complement(1672..1974)
                     /locus_tag="ACHKUB_004652"
                     /pseudo
     CDS             complement(1672..1974)
                     /locus_tag="ACHKUB_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(2001..2423)
                     /locus_tag="ACHKUB_004653"
     CDS             complement(2001..2423)
                     /locus_tag="ACHKUB_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6657567.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKAS"
     gene            complement(2439..3188)
                     /locus_tag="ACHKUB_004654"
     CDS             complement(2439..3188)
                     /locus_tag="ACHKUB_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6657568.1"
                     /translation="MTLPNPLAPVKGSGTTFWVYTGNGDAWANPLSDDDWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEINVTDGAAG"
     gene            complement(3196..3594)
                     /gene="gpU"
                     /locus_tag="ACHKUB_004655"
     CDS             complement(3196..3594)
                     /gene="gpU"
                     /locus_tag="ACHKUB_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO6657569.1"
                     /translation="MKHREIRAAILSALKGNIADRVTWFDGRPVFIDEQELPAVAVYL
                     TDASATEEFVDEGTWEAVLHIEVFLRAKETDSVLDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEYMMWGSADLQWNITYQM"
     gene            complement(3604..4230)
                     /locus_tag="ACHKUB_004656"
     CDS             complement(3604..4230)
                     /locus_tag="ACHKUB_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6657570.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMTAMTHQLRLVLKRKS"
     gene            complement(4233..4514)
                     /locus_tag="ACHKUB_004657"
     CDS             complement(4233..4514)
                     /locus_tag="ACHKUB_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO6657571.1"
                     /translation="MSDLFTRMCCRMDGATVRVMGKQAEINGVVYDVMPEEESADMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(4507..4833)
                     /locus_tag="ACHKUB_004658"
     CDS             complement(4507..4833)
                     /locus_tag="ACHKUB_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO6657572.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     DAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(4921..6900)
                     /locus_tag="ACHKUB_004659"
     CDS             complement(4921..6900)
                     /locus_tag="ACHKUB_004659"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO6657573.1"
                     /translation="MKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFVSELNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVI
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
BASE COUNT         1429 a         2169 c         1688 g         1643 t
ORIGIN      
        1 gccagcaggg atttttcctg cccggataac tgccgtttcg ttgcggcctc tttcaggacc
       61 gcatactgac tttccgcttt ccataaatca cggcgctgct ggctgatttt ctcattcgca
      121 ccgctgtgct tctccagcgt ccggagctca gtttcaagcg ccagcagggc tgcatgcgcc
      181 cggtcttcct gacgctcccc ggctgacaca ctgacacctg acgacttcgg ttttttctgc
      241 gttgattcat aatccttttt cgccgacgcc atcagcgtgt tgtaatccgc ctgcaggatt
      301 ttcccgtctc tcagagcctt gttcagttct gcctgacggg cggtatattt ctccagcggt
      361 gtctgcaggc gttcgtaagc cttctgcgcc tcttcggtat atttcagccg tgacgcttcg
      421 gtatcgctct gctgctgcgc atttttgtcc tgttgactct gctgttcagc cttctttcgg
      481 gcggcttcaa gcgcaaggcg ggccttttca cgatcatccc agtaacgcgc ccgcgcttca
      541 tcgttaacaa aataatcatc cttgcgcaga ttccagatgt cgtctgcttt cttaaacgca
      601 gtctctgcct taatcagcat ctcctgcgcg gtatcaggac gaccaatatc cagaatggca
      661 tcccacatcg atttgaacgc cttccctgtt ttatccgccc aggtttccag cgtccccatg
      721 ttctctttca ggcggcgggt ctggtcatca aaccctttcg ttgcggcctc gttcgccgcc
      781 tgtaaggccc cggcctcatc accggaacgc tgcagttgtg caacatacgc aatctgctct
      841 gccgtcacgt tacggaactg gcgcgccatc gccatcagtc ccaacgtcgg gtcagtggtc
      901 agcttcccga aggcttcagc gactttatcc acctccacac cggatgcaga cgcaaaacgc
      961 gcgacactct ggttgatggc atcaaactgt tcaccaccac gcacaccggc attcaccagg
     1021 gctgccagtg actctcccgc ctggttaaac gtcagccctg ctgcctgccc ggctcttgag
     1081 agagtcagca tacgatcggc agtcagtccg gactgattac cggaaagaac cagggtttta
     1141 ttaaacgctg aaagcgtgga atctccctgg taccaggcgt acaccagcgc acctgtcgcc
     1201 accgccagcg aggtgacccc gaccatcggc agggtgatcg caccggcaag ccccctgaac
     1261 atggggatca tcccgccgaa ggagtccttc acctgaccgc cctgttgcag caggatgagc
     1321 cagggattct gaccaccggc aagctgcgtg gccacgtcgg tgaactgcat gggtagcata
     1381 cgcatggcgg ctttatactg cccgacggac atcccggctt tttgtgcagc cagcgcctgg
     1441 cggctcagcc cctgctcaac agcatcagcc tgttttctga aagactgact gactttcccg
     1501 gacatcaaat cagcaagcgc actggtttca cccagttctt tttttacccg cgctgcctct
     1561 tcagaaaaac gggcagaatc cagtgtcagc acggctgtca gatcggcaaa attacctgcc
     1621 atcgtggtca cctcctgaaa tgtcctctga tgccatcaat aactgtcaca acctccttcc
     1681 ctcctccccg aaacggactc caccagcgag gcccgccgcc ttctgcatca gtacatcatt
     1741 ttcgtccggc gtatccgcct gcccttcctc tgcctccgga gcgaacaggc tgaaatccgc
     1801 cggatgcata tccggatcgc aaaaaaacag gctgagtacg gcgtacgtca gcccggaaaa
     1861 atgcatatcc agctgggtat cctgaaaata atgcgtgcag taaaaacgtc gccagtcggc
     1921 atattcggtg gatgtcatcc cggcaagcat ggcgcgccag tcggctctcc ccatctcacg
     1981 cgccagtttc aggacaaagt tcagctcgcc ttcgaatgct tttttgatgt taccggctca
     2041 gtcgcttctg ctttcccggt tggttcagga tcggcatcgt gccggttatc cagcatacct
     2101 gaaagataaa gcacccggtt cgttgcctga ttcagtgcat cagcaggcca tcccagcatc
     2161 acttcacggc ggatctgctg catctctgtc tccggagagg ccagagtgcc tttgagggaa
     2221 tgggaatgcc atagcgacat cgccacaagc agggatgccg tttccagata tcgctggtta
     2281 atgtgcacgg catcatgctc cgttgtctcc tgttgtgctg cgtctgaaac aaacttcata
     2341 taatcaaacc gctgcagcgc ggacagctcc gacagcgtga cggacacacc gttatattca
     2401 aattgttctg ttttcagaaa catgctttat ctcccccctc agcccgcagc gccatccgtg
     2461 acgttgatct ccgccaccgc cgcaaactca ccattgccgg tgacaacagg gatctgcgct
     2521 ttacctgccg caacaccttt cacggtgatc gtgttcccct tcacggtaat ggtggcgaaa
     2581 ttctgattcg ctgaggttgc gcggaacgtt ttatccgttg cgccttccgg ctgaacggcc
     2641 acggtcaggg tgatattctg accttttgcc acattccccg ttagtggcgt cacggtaata
     2701 ccggtgaccg gtgtgatttc tccccgatcc tcagccagcg acggacgacc aatattggtg
     2761 attttcacag tgcgggtgat cacttctttg gcggtcacgg atttaccaat ggcgctcacc
     2821 cagccacgga acacatccac cgtgccgttc gggaagcgga ttttgtaggc ccgggtctcg
     2881 ctgctgtcaa accaggcaat caaatcacgc tgccctttct cgccgggctt ccaggccagc
     2941 gtaaaactgg tgtcaccggc ggatttctgc ccctgcccgg tggataccca gtcagcatcc
     3001 tcatcatcca gatagttatc gtcgtaggat tccgccgtca tctcgccggg ggtcagatcc
     3061 tttatttttg ccaggcgcgt ccagtcatca tctgacagcg ggtttgccca ggcatcgcca
     3121 tttccggtat aaacccagaa cgtggtgccg gaacctttta ccggcgcaag gggattaggg
     3181 agcgtcataa cgtcctcaca tctggtaagt aatattccac tggagatcag ccgatcccca
     3241 catcatatat tcgtcatccc ggcgatagtc atacccctga agattcatct tcagcagtaa
     3301 tgcactgagc ccgggaactg cctccagcgc cggaagtatt ttttcttcca tccacatatc
     3361 cagcacggag tccgtctctt tcgccctgag aaacacctct atatgaagca cggcctccca
     3421 ggtcccctcg tcaacaaact cctccgtcgc agaagcatct gtcagataaa ccgccaccgc
     3481 aggcagttcc tgttcatcaa taaataccgg acgaccatca aaccaggtca cccggtcagc
     3541 gatatttccc ttcagggcag acaatattgc cgcccgaatt tctctgtgtt tcacattccc
     3601 tccttacgat tttcgtttca gcaccaggcg taactgatgc gtcatggccg tcatcatctg
     3661 cgccggtaat ttttcccggt acatccggtc ccgttcacgt tcaaaggttt ctgccagcgg
     3721 tccggcagtc ggaatcttca ccacttcgat cggcagacgg tggcgtttcg gcctcccttt
     3781 gctgtcagcg ccggtggacg atgatgccca cggcatacgc tgcatcacat gccagcgtcc
     3841 gttagccagc cgggtgataa aggcgtccgg gatccgtctt ttccccacaa tcagcacact
     3901 gccgccccct ttcagggccg cacgctgtcc tttctttctc cgttttctgc gggaaagtcg
     3961 aacgcgggcc tcccccagtt tgatggcggg caggttgccg gtattgatgt aaacctttgc
     4021 gtaaacctta tccggtcgtg ccggacttaa ccggatgcgg gcacggataa gacgacgggg
     4081 aacggccagc tccctggcaa ctgaagaggc cgttttcgca ataatggccc ccgccacgcg
     4141 gttcagtgtc gtggcagagg cccggggaac ggcacggcga tcaattgcat ccagattttt
     4201 catggcctgc gccagacctt ttattgccat actcattcct gttcaacaaa aatccggggt
     4261 ttaccgttgt acgtgtcata acgggtcacc gtcagtgtgc gccccgcaaa cacaacaaca
     4321 tcatgacggg ccggacggta ccgggctgaa aacaccacca gtgacaactg gctgcccgaa
     4381 agcgccccca tatccgcgga ctcttcctcc ggcatcacgt catacactac gccgttaatc
     4441 tccgcctgtt tgcccatcac acgaacggtc gccccgtcca tccggcaaca cattcgcgta
     4501 aacagatcag acattgattt ttaccgccac agtggcgctg tttgcagggg cattttccca
     4561 ggctaccccc gcagccaccg caccgtctgc agccagctgc acaaccccgt ccttcagata
     4621 aaccaccgcg ccggactgaa tatcgtcagc agactgtttg ggcagcagga acacgccttc
     4681 ggcaaaaccg tcaccggcat caccggcagg aatatcggta atggccactg ccaccatact
     4741 gccgaccacc accgcagcac cgctcaggat ggtctgatct ccggcattca ccagttcaat
     4801 ggtggtgccg tcctgtacaa aatttttcgc cataatgctg tttctccgga cagcccctgt
     4861 ggggctgttt ttcaggcata aaaaaagccc tttcgggcag tgattgtgat aacgcggtta
     4921 tcaggccacc gacgaacgca ccagaccgcg ccagtcaagt ggtgccacac cggcatcaat
     4981 acgaattttt gtggcaatgc cgtcagtggt gaaaccttcc tgctgatcaa tgtatggcgt
     5041 gtccacacca tccagccagg ccacttcaat ggtgtcagtg ccctgtgccg ccgccagata
     5101 ccaggttttc ggatctgccg catcaagacg cgcttccgca atcacctcag caaagttctg
     5161 gatagggtta atgacaccgg cgtttgcatc cgcccctttc acactggccg atttgatggt
     5221 ctggttcgcc accgtctcca gtgccaccgg taccagcata aaggccggac ggatattcag
     5281 ggcgcgatcg ccttctttct gcaggcgcat catctgacgg gccgcatcca gtccggaaac
     5341 ggagatcccc ccggtggcaa tatttttgtg atcggcatgg aacagcgcct taccgtcgga
     5401 cagtttcggg ttatccgtca gcaccttgta aaccaggtca ccaatcgttg ccttcgccgc
     5461 acgccccatc ttcatcggca cgtccaccag catattcaga tcatcattga taatggcctg
     5521 gcgggtgatg gagaaaatct ccccgtaggt ggccagagca atggtctcct tgcgatctga
     5581 ggtggtgatg tatttatact ccgccccctc acgaacctgg cgcagagaac caaaaccgcc
     5641 catccccacg cgatacgctg ttttgaagtc tgacaggcgt cccttacggg tccacttctg
     5701 gaaggtttct tctgattcct cccagccctg gatcagcccc ttgttcgaca catccagcag
     5761 gatattgcca aaatcagagg tgctgtgcgt cagcgccagc ccgaccatct gcatggggtt
     5821 ataactggcc accccaatac cgcgctccgt cagtgacatg cgagcccatt cacgcagggt
     5881 catcccgtta taggcgttat ccttctcgac attttcaaat ccggcacggg ccagcatcgc
     5941 ctggcggatc ccgtctccca caaaattgcc gtttccggca taaatatgag ccggtgtgtt
     6001 tttgttggtc ggcgaggact ccttgcccat ttcattcagc agacgttcac gggccatttc
     6061 cagcgaacag tcaggatcag ccacgcactg tgcctgaagc gtctgatagc gaccgccgaa
     6121 catggcaaac agatcgttaa tgcctgacat gcgggctttc tgttctgcca taacgcgggc
     6181 gcgaatggtc gcctcatcag acactgccgg taccggtgat ggttctgtta ccgccggtgc
     6241 agggattatc actgtggtat cacgcggggc actgttgcgt ggcggagtaa tcatgtttcg
     6301 gatggattcc ggcatctttt taaattcctc tgtacgtttt gactgaatac atgccattgc
     6361 ctcaacagcg ggtgtcacct ggtcagcaaa tccgtgtgcc agacattcgg caccggacat
     6421 ccaggtttca tccgccagca tggcggcaat ttcatcggtg gtttttccgg ttttctgcgc
     6481 ataggctggc aacagtaccg attcgacttt atccagcaaa tcggcataac tgcgcatatc
     6541 ctcagcatcc ccgccactga atccccatgg cttatggatc atcatgaagg cattttccgg
     6601 cataatgacc gtatcaccgg ccatcgcaat cacagatgcc atcgaggcgg caacgccatc
     6661 cacatacacg gtaatggtcg ccccctgatt tttcagggca ttaaaaatgg cgatgccttc
     6721 aaagacatcg ccacccggtg aattgatatg gagattaatg tgggtgatat cacccagtgc
     6781 attcagttcg ctgacaaact gcttcgcggt aactccccag aaaccaatct cgtcataaat
     6841 ataaatatcc gcgtcacccg gccccccagc ctgcatcctg aaccaggatt tattcttcat
     6901 gctggctgtc ggtggcctgc tgacgctgt
//
LOCUS       DAZFRN010000218         6710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFRN010000218 DAZFRN010000000
VERSION     DAZFRN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.220"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(97..582)
                     /gene="sixA"
                     /locus_tag="ACHKUB_004660"
     CDS             complement(97..582)
                     /gene="sixA"
                     /locus_tag="ACHKUB_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO6657574.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(785..2929)
                     /gene="fadJ"
                     /locus_tag="ACHKUB_004661"
     CDS             complement(785..2929)
                     /gene="fadJ"
                     /locus_tag="ACHKUB_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO6657575.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2929..4239)
                     /gene="fadI"
                     /locus_tag="ACHKUB_004662"
     CDS             complement(2929..4239)
                     /gene="fadI"
                     /locus_tag="ACHKUB_004662"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO6657576.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4419..4703)
                     /locus_tag="ACHKUB_004663"
     CDS             complement(4419..4703)
                     /locus_tag="ACHKUB_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO6657577.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5075..6415
                     /gene="fadL"
                     /locus_tag="ACHKUB_004664"
     CDS             5075..6415
                     /gene="fadL"
                     /locus_tag="ACHKUB_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO6657578.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1658 a         1822 c         1601 g         1629 t
ORIGIN      
        1 gagagtcttc gatagtagtg aggttgacag atatccgctg cctgatgagc aattatgccc
       61 aatgaatcta cctcattggg cataatttgg aacacatcag atagcttttg ccatcttcag
      121 attacacggg ctcatctgcc agttgaatgt accgtttccg ctttcatcaa gcgttacgct
      181 ggcaatggcc gaggtggtaa acataggcgg cgtttcgccc ggacataact cggcaaccag
      241 atacccgact aacggtaagt gagagatcac cagtaccgaa gcaacacctt cattggtcaa
      301 cgcctgcaaa taggcactga ccagaccaac atcgccgcag ggcgttaact ccggcagaac
      361 ttctgcactg gaaggcaggt tcagacaatc ccctacttct tccagtgttt gctcggctcg
      421 caggaacggg ctcaccagaa cgcgttcgat ttccactttt tgacctttca gccagttcgc
      481 catcaggcga gattcgtcac aaccattagt ggtcagagga cgaaccgaat cactggcggc
      541 atcgagggct gcgtcgccgt gacgcatgat aaaaacttgc atattgcacc gcttttgtta
      601 accagattcg cccgttttct ttcatctgaa accagagaaa gaaaacggtg gccggcattg
      661 tgccttatcc attcaccgaa tgaaacgctg ttttttacct caatggcgta agtatagtca
      721 atatgcgttt acattttgac caacactccg ccattcagcg cggattcata tagctttgac
      781 cttcttattg caggtcagtt gcagttgttt tccaaaaact ttccccacgc gcgcccatct
      841 cgaccaaacg ctcgcaaggg gtaaaacggg aaccatactg cgtggcaagt cgttgcatta
      901 ttgcaaccac ttcgcccgcg ccgagagaat cgatatagcg gaacggtcca ccgagaaatg
      961 gcggaaaacc aatgccaaat accgcgccaa tatccccgtc acgcacgcta cggataacct
     1021 gctcatcaac acaacgtact gcttcattca gcatcaacat cacacaccgt tcagcaacct
     1081 gcggtgcgga gagtcgcccc tgcccttgtg cgccaatgag cgggtaaatg gcaggatcga
     1141 cctgtttttt gcttttacgc cctttctgac cataaagata gaaaccccga ccattttttc
     1201 tgcctttgcg atcgtcgttc aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt
     1261 ctccataagc ggcttccagt acaggaataa ttttagtccc ggtgtcgatt cctacctcat
     1321 ccaaaagttg gattggacct accggaaaac caaatttcac taacgcggca tcaatgtgct
     1381 ctacccgttc accttcggtc agcatgcgga tagcttcatt aatgtaaggc gctaagatgc
     1441 gattgacgta aaacccggct ttgtcacgca cgacaatggg cgttttgccc tgttttttcg
     1501 ccagttttac cgtagtggcg atggtttgcg ccgatgtacc cgcatgagga ataatctcta
     1561 ccagcggcat tttttccacc ggactgaaga aatgcaggcc aataacttgc tcaggtcgcg
     1621 tggcgtgagc tgcgatatca ccaatcggta aagatgacgt attcgaagca aagatggtat
     1681 gagcggcgct attttgctca acttccgcca ccatctgttg tttcaattcg agattttcaa
     1741 acaccgcttc aataatcaga tcgcgatggg caaagccgcg atagtccgtc gttccggaga
     1801 ttaatgccag ctgtttgtca cgttcgctgg ctttgagatg acgacggcga actttgccct
     1861 ccagctgatc ccaactgtac ttcagcgcat gatttatgcc ctgcgggttg atatctttaa
     1921 ttctgaccgg aagccccgct ttacaagcag tgacataagc aataccgccg cccatcaagc
     1981 caccacctaa aattcccacg ctgtttaatg gcgcaggcgg tgcatcacta ccgggatctt
     2041 tcttcacgtc cgtactggca aaaaagatac tacgcagcgc ctgcgattgt ggcgtcatcg
     2101 ccagttcgcc aaacgcccga gcttcggcgt cataaccgct gctggtgccc tgcgctaatc
     2161 ccgtttcaac aacctccagg atgcgttctg tcgccggata attgccttga gttttgtgtt
     2221 ctgttttctt gccgaccatt ttgaacagca gcgcacgacc taacggcccc gccagaatac
     2281 gctcgcgtac aggtagaggg cgggaagatg ggcgatcctt cttcgccagc tcaacagcga
     2341 cttccagcaa aatggagtgc ggaacaacgt catccaccag ccccagcttt aatgcctgtt
     2401 tcgcccgaag ttgttttccg gtgaggatca tctctaatgc tgtgctgacg cctatcagac
     2461 gcggtaaacg ctgggtgccg cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac
     2521 cgagcaccgt tttaggatcg tcagtacaaa cgcgaccgtg gcacgccagc gccaactcca
     2581 gcccaccacc caggcaagcg ccatgaatag ccgcgataac cggaatgggc aaagcatgaa
     2641 tctccgccat caactgttgg ccctgccgcg ccagtgcttc cgcttcttgc gccgttttgc
     2701 agttgccgat catgttgatg tctgcgccag caatgaagtt gtccggttta gcggagacaa
     2761 acaccacgcc tcgcaactct ttgttttcac ggagttgctt aataatggcg cgcacctgcg
     2821 aggcaaactc cgccttcagg gtattcattt tctcacccgg tacgtcgatg gtgataacgg
     2881 caatgttgtc cagacgaaca ttaagggtaa acgctgatgt catttccatt attccgcctc
     2941 cagaaccatt gccgcgccaa gcccaccggc agcacaggcg gtaactaaac caaatccacc
     3001 gccgcgacgg cgaagttcat gcaatgtctg ggtaatcatc cgtgcgccgg tcgccgcgaa
     3061 gggatgtccg taagcaatcg aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc
     3121 agtggcatgt gcacgcccca gtacttcacg agcaaaacgt tcactaccca gcaactgaat
     3181 attcgccagt gtctgagctg caaaggcttc gtgcatatcg atcaatgtca gatcgctcat
     3241 tgtcaaaccg gcacgttcca gcgccagcgg tgttgaccag gccggaccga gcaacatgtc
     3301 ctgccagaca tcaatcgcag taaatgcgta gctgcgcaga taccccagcg gcaccagccc
     3361 taattctttc gcccgggatt cagtcatcag gatcaccgct gccgcgccat cggtcagcgg
     3421 cgtactgttt gccgccgtta ccgttccgtg tttgcgatca aacgccgggc gcagctttgc
     3481 gtaatcggca agcgaggaat taccgcgaat attgttgtct tcgacaagcg gttgtttata
     3541 aggagggata aaggcagtca tcacctcttc tttgagtttt ccgtctgacc atgcctgagc
     3601 ggcacgctga tgcgaacggt gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt
     3661 tttcgccatt tgttcggcgg tgtcgcccat ccgcaagcca gtagaatatt ctgctaccgc
     3721 aggcggtacg ggcattaagt cgcgcaaacg caggcgagag aagagtttca gtcgctggct
     3781 catggttcga gctttgttga catcaaccag cacgcgcgcc agttttttac tgacgccaat
     3841 tggcaatacg gaagaggaat ctgccccacc ggcaatcccc gctcggatag ttcccgccat
     3901 caggctttct gcgacgtttg caaccgcctg gaaactggta gcgcaagcgc ggctgacgct
     3961 gtaagcatcg gtatgcacat tcattccggt accgagaaca atttcacgcg caatgttggg
     4021 ggcttcaggc atttgtacga cctgaccaaa gaccagctgt tcaatcactt cagcggggat
     4081 ctcgctgcgt gccagcagtt cgcctaccac catcttccct aaatcaaccg cgggaatgcc
     4141 atgaaaagcc gtcgcctggc gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg
     4201 atcgccctgg cgggtaacca gcggtaaaac ctgacccata acacacccct gtaaaaaaat
     4261 aaataaagtg gtctgacctg atcatagtct taaccatttt tttacattta gccaagcgga
     4321 gaaaaaggaa agtgggagct atgacacagt gaagacagga gaaagaaaag aaaacgcccc
     4381 tgccagcaac tgacaggggc gcacgtaccc gagaggaatt aacgcagacc cagctggaag
     4441 atcagcattt cagcttcgca ggcaaaagtg aaatcgatat ccaggcgcac accgtcagac
     4501 tcttcagtga aagtcgggga gattttgcac ggttcggatt ccacgctacg ggctttttca
     4561 gtcagcgccg ccagcgtttg ttctgcttct gcgcggtttg caaacacgcg gctgtaagac
     4621 gcggtgcagt cggagttgtc cataatggtg ccaacatcca tacagcagca aaccggggtt
     4681 tcatcagcac tacatttact catcgttgat ttcctctgta tgtgcaccca aggtgccaga
     4741 taaacgttgt ggatatttta cgcttccgga aagtgctgct ccagttgtta attctgcaaa
     4801 atcggataag tgaccgaaat cacacttaaa aatgatctaa aacaaaattc acccgaatcc
     4861 gcgagtgcgc aacctccaaa ttttgccagc tggatcgcgt ttcttagatc atatttgaaa
     4921 aaagatggaa acatacttgc aacattccag ctggtccgac ctatactctc gccactggtc
     4981 tgatttctaa gatgtacctc agaccctaca cttcgcgctc ctgttacagc acgtaacata
     5041 gtttgtataa aaataaatca ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa
     5101 gtctgctctc gcagtcgcag tggcacttat ctccacccag gcctggtcgg caggctttca
     5161 gttaaacgaa ttttcttcct ctggcctggg ccgggcttat tcaggggaag gcgcaattgc
     5221 cgatgatgca ggtaacgtta gccgtaaccc cgcattgatt accatgtttg accgcccgac
     5281 attttctgcg ggtgcggttt atattgaccc ggatgtgaat atcagcggaa cgtctccgtc
     5341 tggtcgtagc ctgaaagccg ataacatcgc gcctacggca tgggttccga acatgcactt
     5401 tgttgcaccg attaacgacc aatttggttg gggcgcttct attacctcta actatggtct
     5461 ggcaacagag tttaatgata cttatgcagg cggctctgtc gggggtacaa ctgaccttga
     5521 aaccatgaac ctgaacttaa gcggtgcgta tcgcttaaat aatgcatgga gctttggtct
     5581 tggtttcaac gccgtctacg ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca
     5641 gttggttgct ggtcagatta tgcaatctcc tgccgggcaa actcctcaag ggcaagcatt
     5701 ggcagctacc gccaacggta tcgacagtaa taccaaaatc gctcatctga acggtaacca
     5761 gtggggcttt ggctggaacg ccggaatcct gtatgaactg gataaaaata accgctatgc
     5821 actgacctac cgttctgaag tgaaaattga cttcaaaggt aactacagca gcgatcttaa
     5881 tcgtgcgttt aataactacg gtttgccaat tcctaccgcg acaggtggcg caacgcaatc
     5941 gggttatctg acgctgaacc tgcctgaaat gtgggaagtg tcaggttata accgtgttga
     6001 tccacagtgg gcgattcact atagcctggc ttacaccagc tggagtcagt tccagcagct
     6061 gaaagcgacc tcaaccagtg gcgacacgct gttccagaaa catgaaggct ttaaagatgc
     6121 ttaccgcatc gcgttgggta ccacttatta ctacgatgat aactggacct tccgtaccgg
     6181 tatcgccttt gatgacagcc cagttccggc tcagaatcgt tctatctcca ttccggacca
     6241 ggaccgtttc tggctgagtg caggtacgac ttacgcattt aataaagatg cttcagtcga
     6301 cgttggtgtt tcttatatgc acggtcagag cgtgaaaatt aacgaaggcc cataccagtt
     6361 cgagtctgaa ggtaaagcct ggctgttcgg tactaacttt aactacgcgt tctgataacg
     6421 cgttcgcctg gataaagtca cctgcatagc aggtgacttt aactccccca cttcaccgaa
     6481 agtagtgtcc tcattgctta cctcctgagt tttgcaaaca gcctgttggc agcttgccct
     6541 ttcaaatcaa taagcggtat ccacagacaa acctgaaggg aaaggcatat tttcaggcgt
     6601 tctgctcgtc cttctcaaag agtttacttt tctgcatttc caggatactc aacccccctg
     6661 gctattgtgc gctcatacac tcaaattaaa gataggttct aaataaatga
//
LOCUS       DAZFRN010000219         6698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFRN010000219 DAZFRN010000000
VERSION     DAZFRN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.221"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(154..390)
                     /locus_tag="ACHKUB_004665"
     CDS             complement(154..390)
                     /locus_tag="ACHKUB_004665"
                     /inference="COORDINATES: protein motif:HMM:NF042477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 6"
                     /protein_id="HFO6657579.1"
                     /translation="MIISDVTKPSQTLYYLGARLLECMSSNAYSYVDIIDLYNEFKSK
                     NNNSSFTQYIYTLNWLYLLGLVDANENGDLILCF"
     gene            complement(387..983)
                     /locus_tag="ACHKUB_004666"
     CDS             complement(387..983)
                     /locus_tag="ACHKUB_004666"
                     /inference="COORDINATES: protein motif:HMM:NF042705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFO6657580.1"
                     /translation="MVGVTQRSGNYSRNVQVVVDSNSVDIIKSPSIFMDLVGILVDSN
                     VIDPRTNNDYEPYDIENKIDFNDVIKHRERIEGFYLYSSMIDKAYETLNESMPTAKET
                     ALKSINSRYLDCQGDLLLKHREAYKSCKDAQERNNLKIQLVREFADWIIDSVIEHIIK
                     KCLNSLMAMNYTSEEIECHASFIVYHAFVECKILEKPI"
     gene            complement(986..1225)
                     /locus_tag="ACHKUB_004667"
     CDS             complement(986..1225)
                     /locus_tag="ACHKUB_004667"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657581.1"
                     /translation="MIRIMIRIFSLTFVACAGFAAYHFNSVPLMFTSGSFLVTFLGTF
                     IVNKKSSGHTIIQKSGSHSKNNQTVVFGNNENKRG"
     gene            complement(1549..1980)
                     /locus_tag="ACHKUB_004668"
     CDS             complement(1549..1980)
                     /locus_tag="ACHKUB_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6657582.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKTEVMQRILAFYQGNARYLLKRRD"
     gene            complement(2021..2986)
                     /locus_tag="ACHKUB_004669"
     CDS             complement(2021..2986)
                     /locus_tag="ACHKUB_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657583.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLNESIGSKCAAPSGQNDTMEEVKMKRSIGSKRSNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPNPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(2967..3488)
                     /locus_tag="ACHKUB_004670"
     CDS             complement(2967..3488)
                     /locus_tag="ACHKUB_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6657584.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTYEGGAVDQRALSVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYESSFC"
     gene            complement(3472..3699)
                     /locus_tag="ACHKUB_004671"
     CDS             complement(3472..3699)
                     /locus_tag="ACHKUB_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6657585.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVRLASVAAWGILVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            3780..4187
                     /locus_tag="ACHKUB_004672"
     CDS             3780..4187
                     /locus_tag="ACHKUB_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6657586.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPNHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
     gene            4355..4507
                     /locus_tag="ACHKUB_004673"
     CDS             4355..4507
                     /locus_tag="ACHKUB_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6657587.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            4519..4884
                     /locus_tag="ACHKUB_004674"
     CDS             4519..4884
                     /locus_tag="ACHKUB_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657588.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPGVLSGEICTEISSELLPVIREAICRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
     gene            complement(4853..5053)
                     /locus_tag="ACHKUB_004675"
     CDS             complement(4853..5053)
                     /locus_tag="ACHKUB_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657589.1"
                     /translation="MTKEEFVSYIFDKTVEMYAATYGSCNPLNKPEGKDDFDKIYRFL
                     EDRYIKRLEDAGIKSPVKSPLS"
     gene            5556..5744
                     /gene="dicB"
                     /locus_tag="ACHKUB_004676"
                     /gene_synonym="ftsT"
     CDS             5556..5744
                     /gene="dicB"
                     /locus_tag="ACHKUB_004676"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6657590.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            5741..5932
                     /gene="ydfD"
                     /locus_tag="ACHKUB_004677"
     CDS             5741..5932
                     /gene="ydfD"
                     /locus_tag="ACHKUB_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO6657591.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            6026..>6698
                     /locus_tag="ACHKUB_004678"
     CDS             6026..>6698
                     /locus_tag="ACHKUB_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6657592.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQY"
BASE COUNT         1851 a         1579 c         1370 g         1898 t
ORIGIN      
        1 aaaaatctaa agctcttaat acagtagttt ttcccacacc atttccagtt tgctctttgc
       61 tggaggtgtt tcggtcaata atcaaattaa cgccaatgtg aaattcgata tcacgaatca
      121 ctccaagcct gaaagaactg actattagcc gtttcaaaaa cataatatca aatctccatt
      181 ttcgttagca tcaaccaggc ctaataaata tagccaattt aaagtgtaaa tatattgcgt
      241 gaatgaagag ttgttatttt tagatttgaa ttcattatac aagtctataa tatcaacata
      301 cgaataagca tttgaactca tgcattctaa taaccgagcc cccaggtaat acaaggtttg
      361 gcttggtttt gttacatcag agataatcat attggttttt ccagaatctt acactctacg
      421 aatgcatgat aaacgataaa agaagcatgg cattcgattt cttcgcttgt gtaattcatt
      481 gccataagag aatttaagca tttcttaatg atgtgctcta ttacactgtc tattatccaa
      541 tctgcaaact ctcttaccaa ttgaatcttt aaattattcc gttcctgagc atccttgcag
      601 ctcttatacg cttctctatg tttaagtaat aaatctcctt gacaatcaag atatcgtgaa
      661 ttgatgcttt tcagtgccgt ttccttagca gtaggcatac tttcatttaa tgtttcatac
      721 gctttatcta tcatcgaaga atatagatag aacccttcaa tacgttctct atgttttata
      781 acatcattga agtctatttt attctcaata tcatatggct cgtagtcatt gtttgtacga
      841 ggatcaataa cattcgaatc aactaaaata ccaacaagat ccataaaaat cgatggagac
      901 tttataatat caacagagtt agaatcgaca accacctgca cattacgtga gtaattccct
      961 gacctttgag taacaccaac catgatcagc ctcgcttgtt ttcattattt ccaaaaacga
     1021 ccgtctgatt attttttgaa tggctaccgc ttttttgaat tattgtatgc cctgaacttt
     1081 ttttattaac aataaatgta cctaaaaatg ttacaagaaa gctaccagaa gtgaacatca
     1141 atggcacact attgaagtga tatgcagcaa agccagcgca agcaacgaat gtcaaagaaa
     1201 atattctaat cataattcgg atcatagcca catcctgtaa gtactgttgt catcttttag
     1261 tgattcttga atcccatact atacaaaagc ttatccatct tttgtacaag cttccgcttt
     1321 gaaagtgaca ttataaagta actacaactt agagtgcatc cccctaaata tctccgatgg
     1381 tgatagttgc tggctacaac ttactttcat aaccaactaa ctggcttgct ctgcgttaat
     1441 tagtgcatag gaatgtcagc attatccgct cttggcacaa accaaacgac cacgctagcg
     1501 ctaatcttgt gccacgaaaa tgtcaattca catctgaact aatgctcttt aatctcgtcg
     1561 cttcaataga tatcgagcat ttccctgata gaatgcgagt atgcgctgca taacttcagt
     1621 cttccggcac tcgcgacaga ttatgtttag gcgactgtcg tagcggcgta tttctccgtc
     1681 aggtaatgac cagataaggt ccggatcaac cacagcaggt ttcttcacct ttgccctcga
     1741 gagttttttg cgggcgtttt gccagtcctt acgcgcctgt tcagacggga ataacccgta
     1801 gccggagttg tatacatcgc cactggctac cagctctctg gcaagaacgc tcatcagata
     1861 tctagtcgca cctgttttag cttccagttg ccgtaacgtc tcacgaccgc tctggcgcac
     1921 gagttccacc acctgccctt taattttttc ccgctcttct tgtgtaaaaa cttttgccac
     1981 aagtccccct taaaattacc tcatgacctg aaatcaacac ttatcctctg aaaccaggcg
     2041 gaatttctgt atccggttca gaaatatgat taacacaacg ctgtacaggt gaacgtccca
     2101 ggcggatgac cagttcgtcc catttttcgc ggagctttga cgggctcatg atgtttttta
     2161 cccagaatgg atctcgctga acccgaccaa acatttcgca aatttgtctg tggcttctgc
     2221 catccagcat ccgcattgtg cgcacgtcat tggcccaggc agtccagtta ggctcttttg
     2281 gtcgcatgat ctcgccatca tcactggctg cctgttcgta gagacccacg atccgccccc
     2341 agatccactg cgcacacgcc aaatcctccc tgctacccca ctggcgtttt ttcgcactaa
     2401 acacaaccgc gtcggagttc cgggttaaaa aatcctgttc agtcgtctgc gggttcggtt
     2461 gcgaagcttc cggacgaaaa gtgtttttat tctctgtagt aatctctgtt gtattctctg
     2521 taagatcatc aggccatttt gacccgatga cattggatcg ttttgaacca atggagcgtt
     2581 tcattttgac ctcttccatc gtgtcatttt gacctgatgg agcggcgcat tttgaaccga
     2641 tggattcgtt caatttgcca ccatctaaaa gctcgctccc atagttgatc gtgtagaaat
     2701 tggtcatatc gcgctttgat ttattgagct tttcacaacg caaaagcccc agcgttttca
     2761 gacttgcaaa cgcgcgcttt aacgttgact ctgaccagaa tgggaactgt tccagccatt
     2821 gttccgttgt gttataaatc cagcgaacac catcacattc cataccggag ttggtatctc
     2881 tcaaccagta gtgcagttgt tgcaaaacaa tggcttcgtt taagccgatt ttcattgcca
     2941 gctgcgtgtt tataaccagt gggcgttcag caaaaagaag actcataatt ccatccagct
     3001 ttttgttggt attgctgtcg atacgcaagc ttgaaagcaa ttgctttttc tataagttcg
     3061 tcagtttcac gatctacaac ggcaggatct gcaaaaagca gtccggattc caccacatcg
     3121 ccatattctt tatttaaccc ggcgatcatg tacgtaatac tttttccatc actgatctca
     3181 cgatacaacc tgaaatcact aatccggata gcctccataa ttgcaggcac tagcgctgtg
     3241 aacttttcac gcttatccct ggtgtcgata gccttccagc gttcgaatat cttcactcga
     3301 ttaacgctaa gcgctcgctg atcaaccgcg ccaccttcat atgtgacacg ctgaacatcg
     3361 atgttcgggc gctctttcaa agcccagaat gcttcagtga ttaatatcgt cgcctgctcc
     3421 tgtgtcattc ctggtcgaca tatccaggca tccagagcct cacgagcctg ttcaggagtg
     3481 attttcattg ttcaaccgcc ccgcccgctt cgtcttacga tattcgtcat aaactttggg
     3541 atcatactga agctccccgc cagatgcctc ctgtagacgc atcgcgcgac cttcaggaac
     3601 cagtatcccc caagcagcaa cacttgccag tctcactcct gcggcattgg caagctttgt
     3661 tttgctgcca aaaaaagtaa ttgcgtcaac tttaagcatc aaagccccct cttgttagac
     3721 ttttctaaca ttattgtgcg cgggatacct aagtcaagaa aaattagaat tacctaacta
     3781 tggatacaag aaccctaggc cagcgagttc tggcgcgacg aaaagaatta cgcttaacac
     3841 aacgagaagc tgcgcgcctc gctggagttg ctcacgtcac aatttcacaa tgggaaagag
     3901 acgaaaccca gccagtcgga aaacgattgt ttgctttagc ggatgctctg aagtgctcac
     3961 ctacatggct aatgtttggt gacgaagaca aggcaccagt gcctgcacaa gaacttcatg
     4021 tggaaacaga gctaactccc aaccacaaag aattgatcga attattcgat gctcttccat
     4081 cttccgagca ggaagccttg ctgtctgaaa tgcgcgcaag agtagaaaac ttcaacaaac
     4141 tcttcgaaga aatgcttaaa gcgcgtaaaa ataaatcaat aaaataacat tcttttcaag
     4201 tgattagttg cgcccactct ttttgttaga taaatctaac aaaaacactt gcctctcatg
     4261 ttaggttatt ctaaattact ttccatcaag acaccgcacg gtgttctcag caaacagttc
     4321 cgcgaccccg gcgttaaggg gaaatgaggt cagcatggat actatcgatc ttggcaacag
     4381 cgaatctctg gtatgtggcg tgttccccaa ccaggacggt acgttcaccg cgatgacata
     4441 taccaaaagc aaaacgttta aaaccgaatc tggcgcgcgt tgctggttag ccagaaacac
     4501 tgactgatga ggctgacgat ggaatttaaa gatttaccgt ctgatgtaca gaaaacagca
     4561 gctcatacat tgcattctgt gctgcgagaa atcgggaaag atattgcaag cgagccagca
     4621 aaagatctgg cccggaaaat caagaccgct ttcgttgagc tttataatgt tggcactgac
     4681 tctgaaactg tcgagaccaa gaccgtaagt tcaccaatat tctcacttga gccaggcgta
     4741 ttatcaggtg aaatatgcac cgagatttcc agcgaactgc ttccggtaat acgagaagca
     4801 atttgccgtc gcggtttgga tggaagttac gatcatgacg tcctgcaagt tctcaggaca
     4861 atggtgactt cactggggat ttgatccctg cgtcctctaa ccttttgata tagcggtcct
     4921 ccaagaagcg gtaaattttg tcgaaatcat ctttcccctc tggtttattc agaggattac
     4981 aggacccgta agtagcggca tacatttcaa ccgttttatc aaaaatataa gagacaaatt
     5041 cttctttagt catatagatt tccttcttgg ttattcggaa taagaaggat accacctcgc
     5101 ctgacgtggt taaaagcagg cacacaacac gaaagcgcac ggcgaagttc gtctcactgt
     5161 acggtgtcgt taaatttaat tcgaccgtgc gcttccggtt gtggcaaccc gcgaaatggc
     5221 gcggcggtaa gtatggcggg gttattcctt ccccgttgag gacaccgagt tgtcaggttg
     5281 accatacgct taagtgacaa ccccgctgca acgccctctg ttatcaattt tctggtgacg
     5341 tttggcggta tcagttttac tccgtgactg ctctgccgcc gtttttaaag tgaattttgt
     5401 gatgcggtga atgcggctaa gcgcacgcgg aacagttaaa accaaaaaca gtgttatggg
     5461 tggattctct gtatccggcg ttaattgtta actggttaac gtcacctgga ggcaccaggc
     5521 actgcatcac aaaattcatt gttgaggacg cgataatgaa aacgttatta ccaaacgtta
     5581 atacgtctga aggttgtttt gaaattggtg tcactatcag taaccctgta tttactgaag
     5641 atgccattaa caagagaaaa caagaacggg agctattaaa taaaatatgc attgtttcaa
     5701 tgctggctcg tttacgtctg atgccaaaag gatgtgcaca atgaattcag catttgcgct
     5761 tgttctgaca gtttttcttg tttccggaga gccagttgat attgcagtca gtgttcacag
     5821 gacaatgcag gagtgtatga ctgcagcaac cgaacagaaa attcccggta actgttaccc
     5881 ggtcgataaa gttattcacc aggataatat cgaaatcccg gcaggtcttt aaaacagttc
     5941 cgtaataaat atccggtttc attcttatat gccagcaatg gcagggattt gttcatcctt
     6001 aaatctgtca tgaggttaaa acaaaatgag taaagtcttt atttgtgccg ctattcctga
     6061 cgaactggca acaagggaag aaggcgctgt ggctgtagcc acagccattg aagctggcga
     6121 cgaacgccgt gctcgagcaa aatttcactg gcagttcctg gaacattatc cggctgctca
     6181 ggactgcgct tataaattta ttgtctgcga ggataaacct ggcatacccc gccctgccct
     6241 cgattcatgg gatgctgaat atatgcagga aaaccgctgg gatgaggagt ctgcttcttt
     6301 tgtcccggtt gagacggaat ccgatccgat gaacgtcact tttgacaagc tggcccctga
     6361 agtacagaac gctgtcatgg ttaagttcga cacatgtgaa aacatcaccg ttgatatggt
     6421 tattagcgca caggaattgt tgcaggaaga catggcaaca ttcgacggac atatcgttga
     6481 agcgttgatg aaaatgccag aagttaacgc catgtatccg gagcttaagt tgcacgccat
     6541 tgggtgggtt aagcataaat gtattcctgg tgctaaatgg cccgaaattc aggcagagat
     6601 gcgcatctgg aaaaaacgtc gcgaaggtga acgcaaggaa accggaaaat acacgtctgt
     6661 tgttgatctc gcccgcgcca gagccaatca acagtaca
//
LOCUS       DAZFRN010000220         6692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFRN010000220 DAZFRN010000000
VERSION     DAZFRN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.222"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            237..497
                     /locus_tag="ACHKUB_004679"
     CDS             237..497
                     /locus_tag="ACHKUB_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO6657593.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            722..796
                     /gene="tap"
                     /locus_tag="ACHKUB_004680"
     CDS             722..796
                     /gene="tap"
                     /locus_tag="ACHKUB_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO6657594.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            789..1646
                     /gene="repA"
                     /locus_tag="ACHKUB_004681"
     CDS             789..1646
                     /gene="repA"
                     /locus_tag="ACHKUB_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO6657595.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            2347..2540
                     /locus_tag="ACHKUB_004682"
                     /pseudo
     CDS             2347..2540
                     /locus_tag="ACHKUB_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2582..2911
                     /locus_tag="ACHKUB_004683"
     CDS             2582..2911
                     /locus_tag="ACHKUB_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6657596.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            2892..3173
                     /locus_tag="ACHKUB_004684"
     CDS             2892..3173
                     /locus_tag="ACHKUB_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6657597.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            3356..3640
                     /locus_tag="ACHKUB_004685"
     CDS             3356..3640
                     /locus_tag="ACHKUB_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO6657598.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            3640..3915
                     /locus_tag="ACHKUB_004686"
     CDS             3640..3915
                     /locus_tag="ACHKUB_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6657599.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(4181..4677)
                     /locus_tag="ACHKUB_004687"
                     /pseudo
     CDS             complement(4181..4677)
                     /locus_tag="ACHKUB_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4954..5829
                     /locus_tag="ACHKUB_004688"
     CDS             4954..5829
                     /locus_tag="ACHKUB_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFO6657600.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLAAGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            5837..6613
                     /locus_tag="ACHKUB_004689"
     CDS             5837..6613
                     /locus_tag="ACHKUB_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFO6657601.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSYATIEIT
                     RKKKSSDGEKRKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVSFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT         1787 a         1680 c         1609 g         1616 t
ORIGIN      
        1 tttcaggggc agacacccga catgccagaa accgccggtc ccgcccgggg ccggcacaca
       61 ggttaaggca tttcctgttt ttcagccatt tcgcaacaaa aatcacatta aacaacagta
      121 atcaccgttg atttgtgcgc caacacagat cttcgtcaca attctcaagt cgctgatttc
      181 aaaaaactgt agtatcctct gcgaaacgat ccctgtttga gaattgagga ggcgagatgt
      241 cgcagacaga aaatgcagtg acttcctcat tgagtcaaaa gcggtttgtg cgcagaggta
      301 agcctatgac tgactctgag aaacaaatgg ccgctgttgc aagaaaacgt cttacacaca
      361 aagagataaa agtttttgtc aaaaatcctc tgaaagatct catggttgag tactgcgaga
      421 gagaggggat aacacaggct cagttcgttg agaaaatcat caaagatgaa ctgcagagac
      481 tggacatact aaagtaaaga ctttactttg tggcgtagca tgctagatta ctgatcgttt
      541 aaggaatttt atggcgggcc acgccgtaag gtggcaggga actggttctg atgtggatgt
      601 acaggagcca gaaaagcaaa aaccccgata atcttcttta actttggcga gtcagaaaga
      661 ttaccggggc catctaaaaa ccgtatagcc aacaattcag ctatgcgggg agtatagtta
      721 tatgcccgga aaagttcaag acttctttct gtgctcgctc cttctgcgca ttgtaagtgc
      781 aggatggtgt gactgatctt caacaaacgt attaccgcca ggtaaagaac ccgaatccgg
      841 tgtttacacc ccgtaaaggt gccggaacgc tgaagttctg cgaaaaactg atggaaaagg
      901 cggtgggctt cacctcccgt tttgatttcg ccattcatgt ggcgcatgcc cgttcgcgtg
      961 gtctgcgtcg acgcatgcca ccggtgctgc gtcgacgggc tattgatgcg ctgctgcagg
     1021 ggctgtgttt ccattatgac ccgctggcca accgcgtcca gtgttccatt accacactgg
     1081 ccattgagtg cggactggcg acagagtccg gtgcaggaaa actctccatc acccgggcca
     1141 cccgggccct gacgttcctg tcagagctgg gactgattac ctaccagacg gaatatgacc
     1201 cgcttatcgg gtgctacatt ccgaccgata tcacgttcac accggcgctg tttgccgccc
     1261 ttgatgtgtc tgaggatgca gtggcagctg cgcgccgcag ccgtgtggta tgggaaaaca
     1321 gacagcgcaa aaagcagggg ctggataccc tgggtatgga tgagctgata gcgaaagcct
     1381 ggcgttttgt ccgtgagcgt ttccgcagtt accagacaga gcttaagtcc cacggcatga
     1441 aacgtgcccg tgcgcgccgc gatgcaggca gaacccgcca ggacattgtc accctggtga
     1501 aacggcagct gacgcgtgaa atcgcggaag ggcgtttccg gggaagcctg gaggccgtga
     1561 agcgcgaaat cgaccgtcgt gtgaaggaac gcatgataat gtcgcgtaac aacaactaca
     1621 cacggctggc cactgcctcc ccctgagttt tacctcatct gaataatccg gcctgcgccg
     1681 gaggcatctg cacgcctgaa gcctgtcagc gaacaaaaaa acagcaccac atacaaaaaa
     1741 caatctcatc atcccgcttc tggtgcatcc ggcccccctg ttttcgatac aaaacatgcc
     1801 tcactgacgg ggaattttgc ttatccacat ttaactgcaa gggacttccc cataaagtta
     1861 caaccgttca tgtcataaag cgccagccgc cagccttaca gggtgcaatg tatcttttaa
     1921 acacctgttt atatctcctt taaaccactt aactacattc atttaaaaag aaaacctatt
     1981 cactgcctgt cctgtggaca gacagatatg cacctcccac cgcaagcggc gggccccgac
     2041 cggagccact ttagttacaa cactcagata caaccaccag aaaaacccaa gtcccgcgca
     2101 gaactgaaac cacaaagccc ctcctcataa ctgaaaagcg gccccgcccc ggcccaaagg
     2161 gccggaacag agtcgctttt aattatgaat gctgtaacta atcatcttca tcgctgtcag
     2221 tcttctggct ggaagtcctc agtacacgct cgtaagcggc cctgacggcc cgctaacgcg
     2281 gagatacgcc ccgacttcgg gtaaaccctc gtcgggacca ctccgaccgc gcacataagc
     2341 tctatcatag ctgacagcgg gtatggcttt gcagggtgga gatgggtaag gtgaaatcta
     2401 tcaatcagta ccggcttacg ccgggcttcg gcggttttac tccggtatca catgtaacca
     2461 cagcgtgccg ccttacatgc gctgacgcgc ataaacaaca agttgctaca ttagcttaca
     2521 ggttaattca ccttgcataa gattagcata taggttaatg tttatcgaaa tgggaggtca
     2581 tatgtttagc gttgtttatc acccggaagc cagggaagaa gccacagcat tacccataaa
     2641 aatcagggtg aagtttgacc gtctcatcag caaactggaa tatgacgccc gattactgcg
     2701 ggaaccggat acaaagcccc tgggggatgg actttttgag atccgcacga tggggacgga
     2761 catagccaga ggtatctggg tataccacaa aggcaatacc atcatcatgc tccgggtttt
     2821 catcaagaag tctcagaaaa cgccagcaaa agaaatcgat ctcgccaaaa agcggttagc
     2881 ggaggtactt aatgaaactg gtaagccata aagaactgca taacgagtgg atgcaggacg
     2941 acgaatatcg cgctgcatgg caggaagaag aacgtaagga gaaactcagg gagttgcttg
     3001 cagaatggcg taagcatgac aatttgacaa aagcgcaagt tgctgaacgc atgggggtta
     3061 ctcctcctgt tatttcccgc ctggaaaaca acatcactaa agccagcatc gacaccctga
     3121 ctcgctatgc ccatgcgtgc ggtatcaaaa aacctgttat tacgctgtac tgaactctgc
     3181 tctatggctg ccagttctgg agaaatcagt aactaaattt taattagcta cgttatggta
     3241 aatctgcctg atgtgtcgat ttctctgaaa gtaattcggc ttctgtgggc tgcgtttgcc
     3301 ttgtgccacc tgaatgtaat cactatgatt aaatatgatt aacaactaat cggatataca
     3361 aatgaaaaac aataccgcac aggcaacaaa agtaattacc gcgcatgtgc cattacctat
     3421 ggctgataaa gtcgaccaga tggccgccag actggaacgc tcccggggct gggttatcaa
     3481 acaggcactt tctgcatggc ttgcccagga ggaggagcgt aatcgcctga cgctggaagc
     3541 cctggacgat gtgatatccg gacagattat cgaccatcag gctgtacagg cctgggcaga
     3601 cagcctcagt actgacaatc cgttaccggt tccacgctga tggaactgaa gtggaccagt
     3661 aaggcgcttt ctgatttggc gcggttatat gattttctgg tgctggccag taaacctgcc
     3721 gccgccagaa tggtacagtc cctgatacag gcaccggtca ttctgttaac tcatccacgt
     3781 atgggggaac agttgtttca gtttgaaccc agggaggtca gacggatttt tgctggcgag
     3841 tacgaaatcc gttacgaact taatggccag actatttatg tattgcgcct gtggcacaca
     3901 cgagaaaaca ggtagattgc gtgtggggac aacttaacat agcctgcaga tattaattca
     3961 ggaacattat tatcaggcca gagattttgc attccattaa ataatcttga cgtataggaa
     4021 attgaaaaac tgagcgatac ccctgtccgc gccttatgcc acgtgagctt ttctgctttt
     4081 cccaccactc cggtgttttc tctctatgta tggtcggttt ttatacctta atgggccgtt
     4141 ttttatactt ttcccggatt gcaggcgcac ctctcctgca tcagccaggg atatacctca
     4201 gcaggcttat atcccgcgcg tttttcttca gccctgatac attcagcccc gcaatggccc
     4261 ggcggtatac catccggcag ccacacagca cgtattcgaa cgggtcacga ctcaggaact
     4321 gcgcttcacc atttgtgcat agcacacttt cgccactgct tccggtttat ccatccccag
     4381 agcacggtac acctgcggca gcttctctcc acacacccgg ttggcaagga acccgaagta
     4441 cctcaccatc ttaaaaaact tctccgggat gtgctgtttc agcctcgcga ccagctcacg
     4501 ctgtgtcagc gtttccgtcg ccgtttctcc cgttttgtgg tccaggtaac ggaagctcag
     4561 gctcgcccct ccgttgtaat gtgccagccg ggaggctgct atcggcggct tcttcagata
     4621 acgacccagg tggcgcgccg tattcctccc gtcggccgtt ttcttcgaca tgtacatcag
     4681 gcgcggaact catcggggcg cgggaatgaa atcccattca gactccggat agattcaagc
     4741 aagcccactt atcgtcaaaa tcggtgttgc aaaaacggga gtgaccatag atccgttttc
     4801 caagcggacc ccataatatc ggctggtaaa catcactcgc tagtgcattc tttttgttgg
     4861 taatgataat tatttacatt tattctttga tgttttatca taattaatgt tttgtcaata
     4921 aatatatgga aacatattgt ttcagatggg aatatgagaa agttcatcct tatttctatg
     4981 ataacgttag ctagtgttag tctgggagcc tgcagacaga atgtcaccat aaagcaggat
     5041 gcgccagggc agaaagcttt cctgactgac gggcagattt acgatttaca cagtggcaaa
     5101 attatttcta gcagcgagct tttggcggat cttgctaccg ctcgacacct tattattggt
     5161 gaaaaacacg ataatgccga gcatcatcag attgagcttt ggcttattca aaatctgctc
     5221 atccagcgac cacaggggag tgtgttgctt gagatgttga cgtcagagca acagccccga
     5281 gttaaccaag ttaaatgttg gctgaaagat aatcctgtgg ttcgagatag ccgtgttcag
     5341 gaactactta actggcaaaa agggtggtcc tgggagatgt atggcgacat cgtcatgcag
     5401 ctattacgtg ggccttaccc gctgctcaat gccaacattg gccgggagca gatgttggca
     5461 ctgtacaaaa aaaacgaatt tcctaaagga aaaaaatcca ctgcacctgt ggtacaagaa
     5521 gctctgcgtg aaaccatcat agctatgcat gaaggcaacc ttgaaagcca gcaactgacg
     5581 tcaatgctct ctgttcagca gcagcgggat cgctacatgg cccgccagct tctgagtgcc
     5641 ccggtaccgt cgttgttgat tgcaggtggt tatcacgcgt caaaaagcat gggcgtaccg
     5701 ttacacatgg aagatcttgc cgccggcacc catccagtgg tgttgatgct tgcagaaaaa
     5761 ggaatgaaca tcacagtaga tcatgctgac tacgtgtggt ttgtagcgcc agatacaaca
     5821 aaaaggtaaa ccatggatga tgaatattct ccacttccca cagtcggtaa aatggagcag
     5881 ctggtttatc tgttctctac tgcttcatgg actgattttc cttgcgttta tctggcgttt
     5941 ttccgaagtt cagcccgcta tgtctcctgc accggcgatc atgctgcaat gggcagaaat
     6001 tatagaggct ccttcttctc cactttcgct tccggtggga atcgctcaac aggagtccgc
     6061 agtgacagaa gaaaagcaac aaactgagga cagacaacaa cgtcctgtca ctgaagacag
     6121 ttatgcaacc attgaaataa ccaggaagaa aaagtcatcc gatggagaaa agagaaagac
     6181 acgtccacct cgtaagataa aggctcaaac cagcgacagc aaccccacag ccgtttccag
     6241 taatgctgca cctcaggctc tggtcgaatc gtcccgtatt gctgcgcctt ttaacagtga
     6301 ctcaacaaag cgagataatt ctgaggcatc ttgggaaagc agagtaaaag gccaccttaa
     6361 ccgttataaa cgttaccctg gcgatgccag aaaacgtgca cgaaccggca cagccgtcgt
     6421 gagttttacc gtaaacacgg aaggcactat tgtcagtagt ttcttggaaa tctcttcagg
     6481 aaccctttct cttgaccgcg aagctattgc tgttttagaa cgcgctcaac cacttcccaa
     6541 accaccgcct gaaatattgg aggggggatt atttaaagta aaaatgccga ttacctttaa
     6601 gttgaaggag taaagggata attggccgtt caagttcatt taactgaaat ttaattttca
     6661 tttttatgct gaacatttta tcaaactttt cc
//
LOCUS       DAZFRN010000221         6667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFRN010000221 DAZFRN010000000
VERSION     DAZFRN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.223"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            129..857
                     /gene="gadW"
                     /locus_tag="ACHKUB_004690"
     CDS             129..857
                     /gene="gadW"
                     /locus_tag="ACHKUB_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO6657602.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1220..4333)
                     /gene="mdtF"
                     /locus_tag="ACHKUB_004691"
     CDS             complement(1220..4333)
                     /gene="mdtF"
                     /locus_tag="ACHKUB_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO6657603.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4358..5467)
                     /gene="mdtE"
                     /locus_tag="ACHKUB_004692"
     CDS             complement(4358..5467)
                     /gene="mdtE"
                     /locus_tag="ACHKUB_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO6657604.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5575..5853
                     /locus_tag="ACHKUB_004693"
     CDS             5575..5853
                     /locus_tag="ACHKUB_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657605.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNVKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5854..6381)
                     /gene="gadE"
                     /locus_tag="ACHKUB_004694"
     CDS             complement(5854..6381)
                     /gene="gadE"
                     /locus_tag="ACHKUB_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO6657606.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1680 a         1615 c         1617 g         1755 t
ORIGIN      
        1 aaaactactt tgtggataaa ttttggtcct accaaatctg gcagtttttg cgctaagaaa
       61 cagtctggca acatttcatt agtatactga aattgaaata atcgcagtat gaaatataag
      121 ggataatcat gactcatgtc tgctcggtga tcctcattcg tcgttcattc gatatttatc
      181 atgaacagca taaaatatcg ctgcataacg agagtatcgt gctgctggag aaaaatttgg
      241 cagacgattt tgcgttttgt tcaccggata cgcgacgact ggatatcgat gagctgacag
      301 tttgccatta cttacaaaat attcgtcagc taccacgcaa tttagggtta catagcaaag
      361 accgtctatt aattaaccag tcacccccca tgccgctggt gacggcgatt tttgatagct
      421 tcaatgaatc cggggtaaat tcaccgatac tgagcaatat gctctacctt tcctgtttat
      481 cgatgttttc tcataagaaa gaactgatcc ccttactttt caatagcatc agcactgttt
      541 caggaaaagt tgaacgcctt attagctttg atatcgccaa acgttggtat ctgcgcgata
      601 tcgcggaaag aatgtatacc agcgagagtc tcatcaaaaa aaagttgcag gatgaaaata
      661 cctgtttcag taaaatatta ctcgcttcca ggatgtcgat ggccagacga ttactcgagt
      721 tacgtcaaat tcctctgcat actattgcag aaaaatgtgg ctatagcagt acgtcgtact
      781 ttataaacac atttcgacaa tattatggtg taacgccaca tcagtttgcg caacattcgc
      841 caggtacctt ttcctgacat attttgcatt tgaatattgg tcaggatctc acacctgctt
      901 catgtgaaac tcttccctga tgatttctgc cgggctaccg gctagttctc tttcgcagtt
      961 tgccaggcgg tataggtatt ttcaggagga aaagactcgg catgtttggg attattaagc
     1021 tgacaattca tacgattacg ggaatgtggg tcagtattgt gctgttcaaa ttgatgacga
     1081 atggctggag tggtttctac tttcagtgct gtgtactaag cctggtattc ctgacggtta
     1141 gctggttgtt gagtggtgaa tggttagcag gaaagagtaa ggctgaacct tcacgttcaa
     1201 ccttactctc atttacacgt tacgcttttt taaagcgggc aaagagatgt tccactacaa
     1261 cgaaaaagac cggaacgaag taaattgcca gcactgttgc ggcaaacatc ccgcccatca
     1321 cgccggtacc taccgcgttt tgcgcgccag aaccggcacc atgactgata accagcggca
     1381 gcacgccgag aataaaggcc agagaggtca tcaggattgg gcgtaaacgc atccgcgccg
     1441 cttcgatgat tgcctctatc ggcgttttcc cttctttctg catcatctca acggcaaatt
     1501 cgacaatcag gatggcgttc ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga
     1561 agtagacgtc attacttaag ccgcgcagat cggtggccag taatgcgcca acgacgccta
     1621 acggaacaac caacatcacc gagaacggaa ttgaccagct ctcatagagt gcggcgaggg
     1681 cgaggaacac cacgaccagt gaaatggcat acagtgcagg agcctggttt gaggataacg
     1741 cttcctgata cgacagtccg gtccatgagt agccgacgcc tgccggaagt ttagcgacca
     1801 ggtctgccat aaatttcata gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa
     1861 tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg tgaaccatag gtccattcgg
     1921 tagacgagta ggcagaaagc ggtgccatcg tgccagaggc gttgcgtaca taccattggt
     1981 tgatgttatc cggcaacata cggaacggcg tgcctgcctg gacatacact tttttcaccc
     2041 gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt
     2101 tgatatcaga cagcgccaca cccattgcct cagctttcgc ggcgttgacg ttcactttga
     2161 acatcggcgt atcttccagg ccgttcgggc gtaccccggt gacctggtca ggcgattgcg
     2221 ctgccagtga taacagctcg tttcgtgcct gggttagttt ttcgtgcccc aggttaccgt
     2281 tgtccagcag ttccatatca aaacctgacg cggtacccag ttcagccacc gcgggtaagt
     2341 tgaacgggaa gacgacggct ttattgatac tgcttaacgc aatcattgcc cgctgaatga
     2401 tcgcggtaac cgagttttcc tcaccgacac gttcagacca cggcttgaga ctgataaacg
     2461 ccaggccgtt gttttgcccc tggccgctga agccaaagcc gccaacggta aacaccgact
     2521 gaacattatc tttctctttt gtaagataat aatccgtcac ctgttgcagc actttcgtgg
     2581 tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt cataaatacc ccctgatcct
     2641 cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac cgccatcccg gcgcaaatca
     2701 gcaggtagac caccatgtag cgaccggtac aacgcaacag cgagcgggtg ctatcggtat
     2761 agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt
     2821 gaccgccttc cggcgcggct ttcagaatgg tggcgcacag ggcaggggtc aggctcattg
     2881 ccacaaatac tgaaagcagc atggaggaga tcagcgtgat ggagaactgg cggtagatct
     2941 ccccggttgc gccgctcata aaggccatcg gcataaacac tgcggaaaga acaacagcaa
     3001 taccgaccag cgcacgttgg atctgcccca tcgatttatg cgtcgcttcc ttcggtggta
     3061 gcttatcttc cgcaatgaca cgctcgacgt tctccaccac cacgatggcg tcatccacca
     3121 gtaaccctat cgccagcacc atcccgaaca tagttaacgt gttgatggtg aaaccgaccg
     3181 ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac ggcaatcgtc gggatgattg
     3241 tggcacggaa attctgcaaa aacagataca tgaccaggaa gacgaggatg atagcctcaa
     3301 ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat
     3361 aaaccgtctt cagacttgcg gggaaatagg ctgataagcg gttcagctcc tctttgactg
     3421 cccgcgaggt atccagggcg tttgctccgg tagccagttt gatggcgatc ccggcagcag
     3481 gtttgccgtt atagcgcgcc acggtggaat aatcttccgc tccaagttcg acgcgggcga
     3541 catcacgcag cagcacttgc gaaccatctt gctgaacttt caacaggatt ttgccaaatt
     3601 cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg
     3661 cctgtggcat gccacccagt tgaccaccgg aaatctggtt gttttgcacc ttaatctggg
     3721 aaataacatc ggaaggtacc agattgtatt tattgagttt ttgcgggtcc agccagatac
     3781 gcatggcgta ctcggaacca aagagctgta cgctaccaac gcccgcggta cggcttagcg
     3841 ggtctttgat attagacgct acatagtccg cgatatcgta ctggttgagg ctgccgttat
     3901 cagaaataaa cgccgctacc atcagaatat tactgctcga cttatcaacg ctaatcccct
     3961 gctgctgcac cgcttcaggt aatgaaggca tagcgagttg cagtttattt tgcacctgaa
     4021 cctgtgcgat atcaggagat gtcccagtct cgaaggtcag agtgatagag gcattacccg
     4081 ccgcatcact ggttgaagac atgtacatca ggccatcaag cccattcata ttttgctcaa
     4141 tcacctgagt gaccgagtct tctaccgttt gcgcatcggc acctggatag gtagcgctga
     4201 cggtaatggt cggtggcgca atctgcggat actgcgcaac cggtaagttc atgatcgcca
     4261 gaccacctgc aagcatcata ataatggcaa gtacccaggc aaaaaccggg cgatcaataa
     4321 aatagttagc catgaaagtc cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg
     4381 cgttttcctg gctggaggaa attgctcgtg ctttgatacc cggacgaatg cgttgcaaac
     4441 cggaaacgat cacccgatcg ccagcctgca agccagaggt gacaacccac tggtcgccga
     4501 tggctttgct ggcttcaatt tcgcgtagct gcacaacatc gtctttatcc agaatgagcg
     4561 ccgttgcttt accctgggcg ttgtgggtga cgccttcctg cggcaccagt aatacattct
     4621 ggcggctacc ttcatccact aatgccgtga cgtacatgcc aggcagcaag tcaccatttg
     4681 ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt ttcatccacc gtcgggtcgg
     4741 agaatttcag cgtgccggtc tggctgtagc gtttaccatt ttccagattg agctgtactg
     4801 gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac ctcttctttc atgcgtaaga
     4861 aatcttgcac cgactgcgtg agatcgacat aaatcgggtc cagacgttgt acggtaacca
     4921 gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt caccgacgat ttcccgctga
     4981 cgcccgtaat cggcgaggtg acattcgcgt attgcagatt gatcgtcgcc tgttcaacag
     5041 ccgctttggc gacggtgaca ttggcttctg cttcattcaa ctgggtgcgc gcggtgtcgt
     5101 aatcctgacg gctaacgtag ttggtcttca gcaacgatgc ctggcggtta aaggtgatgc
     5161 gggcattgct ggcggtagag agcgctttcg ccagcgagcc tttggcggag tttagctcgg
     5221 cctgtaaagg tgcaggatca atctgataca gcgaatcgcc ctggttcact ttatcgcctt
     5281 cgataaagtt gcgtttaatg ataataccgc ccacctgggg acgtatctcg gcaacttcat
     5341 aaggaacggt tctaccgggc aattcgctca acacattgac cgaaccgggg gagagtgtga
     5401 cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc cgatttgtca tcgcaggcgg
     5461 tgagcatcgc gccgcagaat aacaacggta ttaacagctt tcttcttctg ttcattttag
     5521 tccctgaaaa ttcttgagaa ataacggttc tttaaattgc gcgcgaggag agatatgagg
     5581 gatttacaga ccagcggcat agtggggttg tcagccagta aggtgggata caggtatagt
     5641 gatcgacatg gtgaggtcaa cgtcaaaaaa aatgttgccg ttctgccaac agttcctgcc
     5701 agcattcggg caacgaaggg ttctactggt ggatacacat accaggggaa taaagtcgtt
     5761 ttagcctctc ctttagtaat caccgggggt aatgaaatca gcatctgcat ccctcgtcat
     5821 gccagccatc aatttcagtt gcttatgtcc tgactaaaaa taagatgtga tacccaaggt
     5881 gacgatgtcg ctcatacgtc ttagtttcag cttgtacata atgttgaatt tctgaatctt
     5941 taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg ctttcatttt tatttttcag
     6001 aatcagttgc ataattttga actgattatg tgaaaaacat aattgtgaat tcttatgggg
     6061 caagtgttta ccataagtaa tctcaagaag atttttgata ctttctttgc ggctaacaaa
     6121 ataaataggc gctttagctt ttagtttact gatgtgataa ggtgccaaaa caataattct
     6181 ctcggcatct aatttctcca gaaatttaat cgcttcttca tcaaggatat gattgtgata
     6241 ggtatcaatg ataaccttga agggtttatt gcctactttt ttgatctctg acagggaatt
     6301 gatttttatc atttcgtgat tatctttcaa ctgccaaaag ccctgtaaaa gaaaagaatc
     6361 tttcgtcatg agaaaaatca taacttgctc cttagccgtt atcgtttcga acatgtcatc
     6421 cttttcatta tttacatcct tgtccgaatc gttgttcaat atagtaaacg cctataagaa
     6481 ctttgccctt cgtgaactaa tacgtctatt aacactaatc aatacttcag atttatccat
     6541 cacgcactcc atatattgat cctaagtaag attcctggtt gttatcagtt tgtaaacagt
     6601 aaacaaaaag ttaacgtttg cggattactg cccaagaata agttatcaat aaccagtttg
     6661 tgatctc
//
LOCUS       DAZFRN010000222         6565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFRN010000222 DAZFRN010000000
VERSION     DAZFRN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.224"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..80
                     /locus_tag="ACHKUB_004695"
     CDS             <1..80
                     /locus_tag="ACHKUB_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO6657607.1"
                     /translation="IGFANTTFSDADFGALGILLGIIVR"
     gene            complement(296..580)
                     /locus_tag="ACHKUB_004696"
     CDS             complement(296..580)
                     /locus_tag="ACHKUB_004696"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6657608.1"
                     /translation="MEQKTLSAEPRRSFSNDFKLQMVKLALQPRDSVACIAREHDIND
                     NLLFKWLRLWLIKGRISRRLPVTNSSGIGVELLPVEVTLDERPYPVFYAR"
     gene            complement(1001..2014)
                     /locus_tag="ACHKUB_004697"
     CDS             complement(1001..2014)
                     /locus_tag="ACHKUB_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO6657609.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLTGRYEYIMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(2026..3342)
                     /gene="fucP"
                     /locus_tag="ACHKUB_004698"
     CDS             complement(2026..3342)
                     /gene="fucP"
                     /locus_tag="ACHKUB_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO6657610.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(3370..4290)
                     /gene="rbsK"
                     /locus_tag="ACHKUB_004699"
     CDS             complement(3370..4290)
                     /gene="rbsK"
                     /locus_tag="ACHKUB_004699"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO6657611.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQNGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            4596..5378
                     /locus_tag="ACHKUB_004700"
     CDS             4596..5378
                     /locus_tag="ACHKUB_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO6657612.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISDRPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(5380..5606)
                     /locus_tag="ACHKUB_004701"
                     /pseudo
     CDS             complement(5380..5606)
                     /locus_tag="ACHKUB_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT         1922 a         1511 c         1377 g         1755 t
ORIGIN      
        1 atattggttt tgctaacacc accttcagtg atgctgactt tggcgcactg ggtattttgt
       61 tagggattat cgttcgctaa tgttttgatg acattaatat cctccaggat ttcaggaacg
      121 tggaggatat taattcctct tattttctcc atttttcttt cttccagtca ggactgcatc
      181 cgattaatcg taagcgctcc gagaagtacg tagcgtaagg attattttac agctgagaag
      241 tttcagtgca gcagttcatg cacacggttc gacggctagt cattgatctt cataattacc
      301 tcgcgtaaaa aacggggtaa ggccgctcat ccagcgtcac ctccacaggt aataattcaa
      361 cgccaatgcc agaagagttc gttaccggaa gacgccgtga tatacgccct ttgatcagcc
      421 agagcctgag ccatttgaac agaagattat cattgatatc gtgctcccgg gcaatacagg
      481 caacagaatc ccttggttgt aaagccagtt taaccatttg aagtttaaaa tcatttgaaa
      541 atgacctgcg gggttcagct gataatgttt tttgttccat aacaggtgtc cactagttga
      601 aaggtgggta cctatgttac caatactggc ttaatggcta catacagcaa tcggtttacg
      661 catacacttt tatagtgcac tccggcctca tgaatataac ggtggattac cgccgaatga
      721 atcggaaaat agatactgga aaaaaactga aattgtgacc agtttttgtt gaccactaca
      781 aataaataaa agagttgcag tgcttgctcg gtaagaaaac aatggaagtt gagatcctca
      841 aagaagctgt ggagtacacc tctctacagt agttgcgcaa atttggagtg gacgataatg
      901 tgatcgtcta taagggcaac gctatcatag tcttgtcctg gcgggtaaaa aaacgcgctt
      961 accttaacga taagcgcgcc gctgttcagg acttgagtgg ttattcaatt cctgtggtga
     1021 ctgtaaaagt gcgcgtttgc tgcggtgcaa cctgaatcag cgtgccatta cgttgcgcgg
     1081 caagataccc ctcaggccga caggttgcag gtaatgcaaa ggcggctacc tgttgctctc
     1141 cgttataaag gatccagcga gtcacataat ttagttcagc actgtagaaa cgagtaacaa
     1201 acgtagtgcc atcgggagag atcatgcgaa actctggctg atctgtataa gcgtccagtt
     1261 tgtctgcaaa gaagacaatt tctggatcat aaaattccgg ttgactcagc gtcgacagag
     1321 aggcttctcc ctgcataatc cgttgattaa acgccagcca ctgagcggtg ggattaacat
     1381 gcgaaggcac tgattcacgc aatcttaata tttcgtccgg gatattctgg ctgaatgtag
     1441 catttggtat atatgcataa ttcatgtggc acatatattg tagtggcata tctacagaag
     1501 ccagattggt tacggccatc ttaatatcga acagtgtaga ggatttgtga aggaccactg
     1561 ttggttgagc cagataatga tgaccgaaac ccattatata ctcgtaacgt ccggtaaggc
     1621 gtaacatatc tccgtctaat tccagccatg cttcatccat cgcggcacag gccatttcac
     1681 cgtgtagcag atgagtatct tccgcagatg ggcagccatt agccagcaaa cctgaatgaa
     1741 aagcaaaaca gccataggtc tctatcacct ctgtcgccgg tttaggctgg cgaaacatat
     1801 tgcacatggt gaggccgtgt ccatcaaatt gcgcatccca aatcatctgc cccatccagg
     1861 gaagaataat caaatgtcca cgactgtttg caattttaag cccctcgaca ccgctgtcat
     1921 agcgaaaaga cgtgacagta aaatcactat tttccagcaa gatacgaggt ttctcgccaa
     1981 aaagcgcccg ccacaaatta atacgcgtac tcataacggt tctcctcagg acgctgtgac
     2041 ttcagccagt gcggtacgta ctttgctttc acgccagaag tagactccga catagacaaa
     2101 acagagcata gaaaccagga atgaaagctg tagtgagtgg aacatatctg caatatatcc
     2161 ctgaattgcc ggaaccaccg cggcaccgac aatagccata acaatgactg ctcctgccat
     2221 ttctgtatgt tcgttatcaa ctgtatccag tgttcctgca tagatcgtcg cccagcaagg
     2281 gccaaacaaa acacttacca ggacggcgac atagaccgcg ctgaaacttg gagccagtgc
     2341 aacatatgcc aggaacagcg cccctataac ggaatagaga atcaatactt tttccggatt
     2401 aaaacgcgtc ataaggatgt tggctataaa cttgccaata aagaagcagg caaagctgta
     2461 gaccatgaag tttgaagcat cacgttcgtt gatatcgccc aactccagcg ccagacggat
     2521 ggtaaatgac catactgcga cctgcatacc cacataaagg aactgcgcca caataccgcg
     2581 acgaaagcgc ggatttctag ccagatagcg cagcgtatcc attgctgacg ggcgtttatg
     2641 gtgacttgtc tgtgccactt tacaggttgg gaagcgggtt aaaaggaaca acaccatgac
     2701 cacaaccaga atcataatca tatacttata cggttcaagg gtgttctcta acatcagcac
     2761 cttaaagttg tgaatttgcc cggcgttcat tccggacatc tgcttctcaa ggctttcccc
     2821 ctcggagaaa accagatatt tgcccaataa aataccagac gcagcaccaa tcggataaaa
     2881 ggtctggctg atattgagcc gcaatgtggc ataggctttt ggaccgatca ttgaactgta
     2941 tgtgttcgct gcagtttcaa ggaaactcag gccaatcgca atcgcaaaaa tagctgcaag
     3001 gaacatagtg taggttgcca tatgcgaggc agggaaaaaa agtgtacaac caccaatata
     3061 cagcgtcagg ccaattaaaa ttgccacctt ataactggtc tttttaatca caagggatgc
     3121 tggtattgca attaaaaaat aaccgccata aaatgcgctc tgcaccaatg ctgaagcaaa
     3181 gttacttagc gaaaatacac ttttgaattg agtgattaat atgtcattta atgcagctgc
     3241 gcatccccat agcgggaata aacacgataa caaaataaac tggaacaagg gagtcttatt
     3301 cagataccca tcaggcatct gaatgatgtt tttatcgttc atagtgctac ctttaactgt
     3361 gcaggatgat tattcgttta aggttaaaaa ttcattaaat tgttcaatac tcggataaga
     3421 tgattgcgta cctttccctg tgacgctgaa agcggcaaag agagcggctt ttttcaaagc
     3481 ggcttcaaca tcaccgtttt gaacataata atgggaaaag caaccaataa atgcgtcacc
     3541 agcgccacta gtatcaacag catttacttt gaatgcagga acatggactt cctgatcgcg
     3601 ggtcatccat aatgcgcctt tttcgctcat ggtaacaata atattgttca gccctttatc
     3661 aactaacgaa cgtgcggcca aacgaatatg atcataagta tcaaccgaca taccggttaa
     3721 tatttccagt tctgtttcat tcgggataaa gaaatcacat ttgcaggcat aagacatatc
     3781 taactcacgc aatgccggag ccggatttaa taacacttca ataccatttt tcttaccaaa
     3841 ctcaatcgcg tggtaaactg tttccagttg aacttccagt tgtaaaacga tcaatttgca
     3901 ttttttcaga tcttctgccg ctcgatcgat atcttccggg gaaagaaatt tattcgcccc
     3961 cttaattatt aaaatactat tgctcgagtt ggcattaaca aagatcggtg caacaccact
     4021 gctggtacag gggaccttct caacataagt ggtattaatt ccccatgatt cgagattacg
     4081 aatagtatta tccgcaaaaa tatcatcacc tactttagtc agcatcagga cttttgaatt
     4141 caatttagcc gccgccaccg cttgattagc acctttccca ccacatccga ttttgaaggc
     4201 aggtgcttcc agagtttctc cttctttagg catctgatta gtgtaagtaa tgagatccac
     4261 catattggaa ccaataactg caatgtccat ttcactacct cttataaact ttcgcataac
     4321 aatggtattt aaataacatt agcatgttac ttttgcatca tttgtgactg agatcgcgat
     4381 tagcacatca acccgatgtt tatttaatag acttccagtc ttatcactca ggccaacact
     4441 atctaatcat aagcaaccta acaggattaa taccgaaaat tcagcagtct ataccctttt
     4501 catttcaaag ggtcggtcgt atagtatggt aactaaaaca atgtttacta atgccataat
     4561 gttattttta taacattttt acggagagag agttgatgga aacgaagcaa aaagagcgta
     4621 tccgacgttt gattgaaata cttaagaaaa ccgacagaat ccatttgaaa gacgcggcac
     4681 gaatgctgga agtttctgta atgactattc gtcgcgatct ccatcaggaa gatgaacctc
     4741 tgccactgac cctactgggt ggctatattg taatggtgca taaacccgca ccatcgatgc
     4801 cagtaatcca ggacgttccg agaaatcatc gtgatgactt acctattgca attctggccg
     4861 ccggaatggt taatgaaaat gatctgatct tctttgataa tggccaggag ataccgctcg
     4921 ttataagcat gatcccggat gcaatcacct tcactggcat ctgttactca catcgtgtct
     4981 ttgttgcgtt gaatgaaaaa cctaatgtga cagcaatact ttgtggtggt acgtatcgtg
     5041 ccagaagtga tgctttttac gatgccagta actcttcgcc attagactct ctcaatccgc
     5101 gaaaaatatt tatttccgcc agcggtgtac atgatcactt tggcgtcagc tggtttaatc
     5161 ccgaagatct tgccactaag cgtaaagcga tggcccgtgg actacgaaaa attttgctcg
     5221 cccgccacgc cttgttcgat gaagtagcct ctgccagcct cgcaccgctc tcagcatttg
     5281 atgttctgat tagcgatcgt ccgttaccgg cagattatgt tacgcactgc cggaatgctt
     5341 ctgtaaagat cattacacct gattcagaag acgaatgact tactgaaaaa acaccgtact
     5401 cttgttaaac atcgtcggat tggactgatt acgttgcact ttcatcacat attccagctt
     5461 atcaatttgg cttatcatct actccagacg ctggtcatca ttgaccaata gtctgatatg
     5521 gcttttgtcg ctgtcctgaa tcggcaggca gaaaatgcct tcaacgttaa aagcgcgacg
     5581 ggaaaaagcc cacaaaggtg agtcattacg cccggataat aaacaggctc ctccctgtta
     5641 tggaagccgt tgtattgtcc ataatgtctg ggtttttaat gcacactgga taacaaccgg
     5701 acgaatcttc aaatatcact ggcgctgaca gccggtttga attcatctca caaccctgca
     5761 tagagcgaat ctcctgcctg cacgtcactc cactccatgg tataaacttc acactcttta
     5821 tctgcggcta gtttcagtca ccagataagc atcgactatg aaaggtgagc catgacaaca
     5881 taacgttggt aacgctctga cgccttaacg gaagatgcct gccaccacag ggaatgtaaa
     5941 cgactgaagt gtggccttta atgccgtgaa cggctcatgg tctcctggca cggttgccgc
     6001 cccaacctgt aacaacattc cacagtacaa tgtctgtcag agccggagcc tcccatgcct
     6061 gttgtagtaa ctctaccagt tacgaacatc cttcttcaaa agtgttgtcg tatctcgcat
     6121 ggaagaaaag atcctgacta agcagcaaca cacaacgtat tgcggaacct gcatgttttt
     6181 cctgtaacca gtgtattacc acatatggta atagctacct gtgtggtttc gctggataac
     6241 aaggggattt attcgcaagt aaaatgcctg ataaaataca cgaatctagt aatcatcaat
     6301 atttactctg gtcgaatgac gcgtgaagtg gacggccaac agacgcagtc agtgatctac
     6361 agcctgttga ataaaacgct ggatgtctcc cggctctgaa attaatgctt cggttatttg
     6421 cacgaaatgt tccttttcaa accctgttga attttgttaa gccggtgcag acacagtaaa
     6481 gcgcgcatca acacctgaaa ggtacgatac aaacacaagt tacaaacaat aataacggaa
     6541 ggacaattaa tcagtgagga ataat
//
LOCUS       DAZFRN010000223         6548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFRN010000223 DAZFRN010000000
VERSION     DAZFRN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.225"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            212..4465
                     /gene="fdeC"
                     /locus_tag="ACHKUB_004702"
     CDS             212..4465
                     /gene="fdeC"
                     /locus_tag="ACHKUB_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO6657613.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNLVTLTVVDTYGNPL
                     QGQEVTLNLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVGTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            complement(4586..5443)
                     /locus_tag="ACHKUB_004703"
     CDS             complement(4586..5443)
                     /locus_tag="ACHKUB_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6657614.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            5692..>6548
                     /locus_tag="ACHKUB_004704"
     CDS             5692..>6548
                     /locus_tag="ACHKUB_004704"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6657615.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAV"
BASE COUNT         1774 a         1469 c         1727 g         1578 t
ORIGIN      
        1 cacccataaa taaaaatata atcccgcgcc caaccacctg atgagtggct acagacactg
       61 gatatattag gtggcggtgc actttcttac ataaaggtat ttcattttct gcggaaaagg
      121 aaatcgggaa atccccggtt tttctgacaa gcagacgcca ttatttgtgt ctgcctatgt
      181 tcgttaattc gttcatcagg aaattatctc aatgtcgcgt tataaaacag gtcataaaca
      241 accacgattt cgttattcag ttctggcccg ctgcgtggcg tgggcaaata tctctgttca
      301 ggttcttttt ccactcgctg tcacctttac cccagtaatg gcggcacgtg cgcagcatgc
      361 ggttcagcca cggttgagca tgggaaatac tacggtaact gctgataata acgtggagaa
      421 aaatgtcgcg tcgtttgccg caaatgccgg gacattttta agcagtcagc cagatagcga
      481 tgcgacacgt aactttatta ccggaatggc cacagctaaa gctaaccagg aaatacagga
      541 gtggctcggg aaatatggta ctgcgcgcgt caaactgaat gtcgataaag atttctcgct
      601 gaaggattct tcgctggaaa tgctttatcc gatttatgat acgccaacaa atatgttgtt
      661 cactcaggga gcaatacatc gtaccgacga tcgtactcag tcaaatattg gttttggctg
      721 gcgtcatttt tcaggaaatg actggatggc gggggtgaac acctttatcg accatgattt
      781 atcccgtagt catacccgca ttggtgttgg tgcggaatac tggcgcgatt atctgaaact
      841 gagcgccaat ggttatattc gggcttctgg ctggaaaaaa tcgccggata ttgaggatta
      901 tcaggaacgc ccggcgaatg gttgggatat ccgcgcagag ggctatttac ctgcctggcc
      961 gcagcttggc gcaagcctga tgtatgaaca gtattatggc gatgaagtcg ggctgtttgg
     1021 taaagataag cgccagaaag acccgcatgc tatttctgcc gaggtgacct atacgccagt
     1081 gcctcttctg acactgagcg ccgggcataa gcagggcaag agcggtgaga atgacactcg
     1141 ctttggcctg gaagttaatt atcggattgg cgaacctctg gagaaacaac tcgatacaga
     1201 cagcattcgc gagcgtcgca tgctggcagg cagccgctat gacctggttg agcgtaataa
     1261 caacatcgtt cttgagtacc gcaaatctga agtgatccgt attgctctgc ctgagcgtat
     1321 tgaaggtaag ggcggtcaga cactttccct ggggcttgtg gtcagcaaag caactcacgg
     1381 actgaaaaat gtgcagtggg aagcgccgtc attactggct gaaggtggca aaattaccgg
     1441 tcagggtagt cagtggcaag taacgctccc ggcttatcgt ccaggcaaag acaattatta
     1501 tgcgatttct gcggttgcct acgataacaa aggcaatgcc tcaaaacgcg tgcagacaga
     1561 ggtggtcatt accggagcag gtatgagtgc cgatcgcacg gcgttaacgc ttgacggtca
     1621 gagccgtatt caaatgcttg ctaacggtaa tgagcaaaga ccgctggtgc tgtctctgcg
     1681 cgacgccgag ggccagccag tcacgggcat gaaagatcag atcaagactg aactaacttt
     1741 caaaccggct ggaaatattg tgactcgtac cctgaaggcc actaaatcac aggcaaagcc
     1801 aacactgggt gagttcaccg aaactgaagc aggggtgtat cagtctgtct ttactaccgg
     1861 aacgcagtca ggtgaggcaa cgattactgt tagtgttgat ggcatgagca aaaccgtcac
     1921 tgcagaactg cgggccacga tgatggatgt ggcaaactcc accctgagcg ctaacgagcc
     1981 gtcaggtgac gtggttgctg atggtcagca agcctacacg ctgacgctga ctgcggtgga
     2041 tactgatggt aacccggtga cgggagaggc cagccgcttg cgatttgttc cgcaagacac
     2101 taatggtgtc accattggta caatttcgga gataaaacca ggcgtttaca gcgctacggt
     2161 ttcttcgacc cgtgccggaa acgttgttgt gcgtgctttc agcgagcagt atcagctggg
     2221 cacattacaa caaacgctga agtttgttgc cgggccgctt gatgcagcac attcgtccat
     2281 caccctgaat cctgataaac cggtggttgg gggtacagtt acggcaatct ggacggtaaa
     2341 agatgcctat gacaaccctg tgaccagcct cacgccggaa gcgccgtcat tagcgggtgc
     2401 cgctgctgta ggttctacgg catcgggctg gacaaataat ggtgatggga cgtggactgc
     2461 gcagattact ctcggctcta cggcgggtga attagaagtt atgccgaagc taaatggaca
     2521 ggatgcggca gcaaatgcgg caaaagtaac cgtggtggct gatgcgttat cttcaaacca
     2581 gtcgaaagtc tctgtcgcag aagatcacgt aaaagccggc gaaagtacaa ccgtgacgct
     2641 ggtggcgaaa gatgcgcatg gcaacgctat cagtggtctt tcgttgtcgg caagtttgac
     2701 ggggaccgcc tctgaagggg cgaccgtttc cagttggacc gaaaaaggtg acggttccta
     2761 tgttgctacg ttaactacag gcggaaagac gggcgagctt cgtgtcatgc cgctattcaa
     2821 cggccagcca gcagccaccg aagccgcgca gttgacggtc atcgccggag agatgtcatc
     2881 agcgaactct acgcttgttg cggacaataa ggctccgacc gtcaaaacga cgacggaact
     2941 caccttcacc gtgaaggatg cgtacgggaa cccggtcacc gggatgaagc cagatgcacc
     3001 agtgtttagc ggtgccgcca gcacggggac agagcgacct tcaacaggag actggacaga
     3061 aacaagtaat ggggtctacg tggcgacctt gactctggga tctgccgcgg gccagttgtc
     3121 tgtgatgccg cgagtgaacg gccaaaatgc cgttgctcag ccactggtgc tgaatgttgc
     3181 tggtgacgca tctaaggctg agattcgtga tatgacggtg aaggttgata accagctggc
     3241 taatggacaa tcgactaacc tggtaaccct gaccgttgtg gacacctatg gtaacccgtt
     3301 gcaggggcaa gaagttacgc tgaatttacc gcagggtgtg accagcaaga cggggaatac
     3361 agtaacaacc aatgcggcag gtaaagcgga tattgagttg atatcaaccg ttgcagggga
     3421 acttgaaatc gcggccgcgg tgaaaaactc tcagaagacg gtcacggtga aattcaacgc
     3481 ggatgccagc accggtcagg caaacctgca ggtaggcacc gccgttcaaa aagtagcaaa
     3541 cggcaaagat gcctttacgc tgacggcgac cgttgaggat aaaaatggta accctgttcc
     3601 agggagcctg gtgaccttta atctaccccg gggtgtcaag ccgcttacag gcgataatgt
     3661 ctgggtgaaa gccaacgatg aggggaaagc cgagttgcag gtggtttcag tgactgccgg
     3721 aacgtatgag atcacggcat cggcggggaa tagccagcct tcgaatacgc agactataac
     3781 gtttgtagcc gataaggcta ccgcaaccgt ctccggtatt gaggtgatgg gcaactatgc
     3841 gctggcggac ggcaaagcca aacaaacgta taaagttacg gtgactgatg ccaataacaa
     3901 tttggtgaaa gatagcgaag tgacgctgac tgccagcccg gcttcgttaa acctggaacc
     3961 gaatggcact gcgacaacga atgagcaagg gcaggctatt ttcaccgcta ccactactgt
     4021 tgcggcgaca tacacactca aggcgcaagt gagtcagacc aacggtcagg tatcaacgaa
     4081 aactgccgaa tctaaattcg ttgcggatga taaaaacgcg gtactcaccg catcatctga
     4141 tatgcaatct ctggtggcgg atgggaaatc gactgcgaag ctggaggtga cactgatgtc
     4201 ggcaaacaac cccgttggcg ggaatatgtg ggtcgacatt cagacgccgg aaggggtgac
     4261 ggagaaggat tatcagttcc tgccgtcgaa aaatgaccat ttcgtgagcg gaaaaatcac
     4321 gcgtaaattt agtaccagca agcctggtgt ctatacgttc acatttaacg ccctgaccta
     4381 tggcgggtac gaaatgaagc cagtgacggt gaccattacc gcggtggatg ccgatacggc
     4441 aaaggacgag gaggcgatga aataatttta agaatatgaa agtaacattc taataaacaa
     4501 taatggcgtt gacatcttca acgccattat ttattgaatt tcctgacagt agtctcgtta
     4561 taaattcatt tagatatagc gaaaactaaa ccggacgaac ggggataaaa atttcgcaaa
     4621 caacatcatt gctatccttt tctacgaaat cttcattgta ataaaaccgc tcaatatcac
     4681 tgccgttcac tctggctaac cccctggcgg gtaattccat cagatagagg tttcgcgccc
     4741 attcagggta gtgctcccac tcaccttcat agcggaaaga ggcatacagt cctcggttaa
     4801 ttgtccactc tttcggcatc tctgaatcgg tttgtttgca taccgtaccg gcaaaagttt
     4861 caaccgtgag caaatcgacc gattttgttg acggttcaaa cgtggagacg aaatagatat
     4921 ccgtcttaaa attattcata catctggcta aatgatgacg tcgcagtttg acttcttcat
     4981 ccggtgattt attcgacact tcaatgtcat atttaaatga gttgccaaaa ataggtgtct
     5041 caggaaaatt gactaatcta cactccgtct tttgctgttg acgtattaaa aaagaaggga
     5101 agatatttgc gagatcccaa taatcacggt ggcggtattc acggggagag cagccgaata
     5161 actttttaaa ttcgcggctg aatgactgct gtgaatcaaa atgcaaagag agtgcaatat
     5221 caagcataga tttcgcggtg agccggacaa gaatagcggc acggcaaagc cttcgtttgc
     5281 gaatgtattc tcccaaaggc acatgcatga aatttcggaa cagaagctgg atgttgcgtc
     5341 tgctgtagcc agatttctgt gcgatatctt cgattgaaat agggtgctca agattgcact
     5401 caatccactc caggagctgc tgtagaatct tctgcctgat catcacattc tccgttaatt
     5461 ctttgtcata tcgcacattt tcgttatgcc ctgggcccta agtgtatcaa aaacagcaag
     5521 ggcgaaggca aaagcactgg tagcgtctgc ttcgttgtcc ccaaaaatag agaggtttca
     5581 gtcctacatt attaatgaat tttttgcata agtgatatca aaatccacgt actaatttga
     5641 ggttacgttt taacgtagac tcattgttca tgcctaatgg agggactgac agtggaattt
     5701 tccgtattaa gtaacaatct gaaaatgccg atgatgggat ttggtgtttt tcaggttacc
     5761 gataaaaacg tgtgcaaaca gtcagtgttg aacgctatcc gcacaggtta tcgactcatc
     5821 gacactgcag cggtatacgg taatgaagat gccgtcggtg aagccgttcg tgaagctata
     5881 tctgaaggtt tatgtacccg agatgagtta tttataacgt ctaaattgtg ggtgcaggat
     5941 atgttgaatc aggatacggc agcagcaggt attgaagcat cattaaaaaa atcgggacta
     6001 gagtacttcg acctctattt attgcaccag gctatgcgcg attatttcag tgcgtggcgt
     6061 gcactcgaag atgcctatga agagggcaaa ttaaaagcaa ttggggtttc caatttctat
     6121 cctcatgttc tggcgaactt ttgtgaaacg gtaagagtta aaccgatggt caaccaggtc
     6181 gagttgcatc cctattttgc ccaaccagag gcgctggcaa ccatgaagta ttataacgtg
     6241 cagcctgaag catgggctcc gttaggtggt ggacgacata aaccctttga aaataatctg
     6301 cttcagagta ttgcagatgc ccatcaaaaa tcgatttctc aagtcattct gcgttggaat
     6361 attcaacggg gagtggtcgt tattccgaaa tcgacacatc aacagcgtat cgaagaaaat
     6421 tttgctatct gggatttctc actgacagag aaagaaatgg cacaaattag ttcgcttgat
     6481 ttgggttatg ttggggagtc ggtaaaacat tttaatcctg aatttgttcg tggttgtctt
     6541 gctgtaaa
//
LOCUS       DAZFRN010000224         6439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFRN010000224 DAZFRN010000000
VERSION     DAZFRN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.226"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..335
                     /gene="ydjO"
                     /locus_tag="ACHKUB_004705"
     CDS             <1..335
                     /gene="ydjO"
                     /locus_tag="ACHKUB_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO6657616.1"
                     /translation="FKKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPD
                     SSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAP
                     IQNGPLPA"
     gene            complement(339..1730)
                     /gene="tcyP"
                     /locus_tag="ACHKUB_004706"
     CDS             complement(339..1730)
                     /gene="tcyP"
                     /locus_tag="ACHKUB_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO6657617.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1863..2453)
                     /gene="ydjM"
                     /locus_tag="ACHKUB_004707"
     CDS             complement(1863..2453)
                     /gene="ydjM"
                     /locus_tag="ACHKUB_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6657618.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2616..3284)
                     /gene="hxpB"
                     /locus_tag="ACHKUB_004708"
     CDS             complement(2616..3284)
                     /gene="hxpB"
                     /locus_tag="ACHKUB_004708"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO6657619.1"
                     /translation="MSTPRQILAAIFDMDGLLINSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3431..3967
                     /gene="yniB"
                     /locus_tag="ACHKUB_004709"
     CDS             3431..3967
                     /gene="yniB"
                     /locus_tag="ACHKUB_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO6657620.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4008..4868)
                     /gene="yniA"
                     /locus_tag="ACHKUB_004710"
     CDS             complement(4008..4868)
                     /gene="yniA"
                     /locus_tag="ACHKUB_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO6657621.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4974..5264)
                     /gene="ghoS"
                     /locus_tag="ACHKUB_004711"
     CDS             complement(4974..5264)
                     /gene="ghoS"
                     /locus_tag="ACHKUB_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6657622.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5365..6294)
                     /gene="pfkB"
                     /locus_tag="ACHKUB_004712"
     CDS             complement(5365..6294)
                     /gene="pfkB"
                     /locus_tag="ACHKUB_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO6657623.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1676 a         1597 c         1569 g         1597 t
ORIGIN      
        1 gattcaaaaa aaatgagagt ctcctaaagt ttccttcgat aatatcaatg cttaatatca
       61 atcttctttt taatccacta aatcttccgg gaatgggaag tggtgtgctt gaagatatta
      121 tgagtattcc agatagttca cttagaaaac gtctcgggta tgaggtttta agcttctcac
      181 tacaggcaca ttcattgagc caggaatgta ttgataagtt ggacattttt ttcgctgacg
      241 atctgtttaa atatgagtca gtatgcatcg ccgcgatgga acatttaaaa agtaaagcaa
      301 cggcccctat acaaaacgga ccgttgccag cataagaatt aacggtgtgc cagttcggcg
      361 tcgtcttcgc tatccagaat ggctttatcg gtttgcttca gccactggct ggtcagcgtg
      421 ccagctgtca tcgagccact aacgtttagc gccgtacggc ccatgtcgat aagcggttca
      481 acggagatta acagcgccac cagggttact ggcaggccca tcgcaggcag cacaatcagt
      541 gcggcgaaag ttgcaccacc accgacaccg gcaacgcctg cggaactaac ggtaacaata
      601 ccgaccagcg tcgcaatcca catcgggtcc agcgggttaa tgccaaccgt aggcgcaacc
      661 atcaccgcca gcattgccgg atacaaaccg gcgcagccgt tctgaccaat ggtcgcaccg
      721 aaagaggcgg cgaaactggc gatggattca ggaacgccca gacgacgcgt ttgtgcttcc
      781 acattcagtg ggatagacgc agcactggaa cggctggtaa aggcaaacgt cagcacaggc
      841 cataccttac ggaagtactt cagcggactc acgccattaa tgcccagcag aatgccatgc
      901 actgcaaaca taatcagcag accgaggtag gacgcgacaa cgaaacttcc cagtttgatg
      961 atgtcttgca ggttagaacc tgcaaccact ttggtcatta gtgccagaac gccgtaaggg
     1021 gtcaactgca tgaccaggcg aaccagtttc atcacccagc tttgcagggt atcgatagcg
     1081 gttaagacgc gttcaccttt cggcgcatca tccttcagca gtttcagcgc agctacaccg
     1141 aggaatgcgg caaaaattac cacgctgata attgacgtcg gattggctcc ggtcagatcg
     1201 gcaaacgggt ttttcgggat aaaggacaag accagctgcg gaacgctcag gtcagagact
     1261 ttaccaacat agttactttc gatggcgttc agacgtgcag tttctgcacc accctgaacc
     1321 agaccttcag ccgtcaaacc aaacaggttg gtgaccagca caccgaccag cgccgcaatc
     1381 agtgtggtaa acaaaagcgt accgatggtc agaaaactga ttttgcctaa ctgagatgcg
     1441 ttatgcagac gggcaaccgc gctcagaata gaggcgaaga ctaacggcat aacgatcatt
     1501 tgcagcagtt gaacatagcc gttaccaacg atgttaaacc actgtacaga atctttaagt
     1561 acctggctgt cagaaccata aatggtatgc agggcaaggc caaaaaccac acccatcacc
     1621 agacccacca gcactttttt cgccagactc cactgtttgt ggcgggtctg agccagcgca
     1681 aacagcagta caacgaacac cacgatgttc gcaattaatg gaaagttcat ccccgttctc
     1741 ctgatcttat tatttctatc actgcgtagc agtcatagtg ttggcgcaag attaacagaa
     1801 ctgtgagctg gctcttatat tcaaatggaa ttgcttattc cagaaaggtg atttttgcct
     1861 ttttagtatt caacctgatg cttaataagc cgatgaaact ggatctgcaa ggtattgatc
     1921 atttgcgatg accaacgaac cgcgctgttt tcgggtaatg aatggggcat ccataccgac
     1981 cagacaaata atgccatgca gataaaacgt tccagttggt tgcccttttg gggaaccagg
     2041 ataggcaagc ggaaacgcca gcgacatggc cagagcaggg gaacaccggc gggtgtcagc
     2101 atatcggcaa gtatgtggct taaataaccc agcaccattc cttgtagcgc atcagccgga
     2161 atgaaccagc tttcaggaac cttaaggtaa aaggttgcca gcagcgcaaa taccgccagc
     2221 agactgtggg taaaaccacg atgcccaaaa gcgcgggcga tcggttttga tatccacttt
     2281 aatcgctgcc caagaaacga ctttgggtga tcgatgtccg gtaacaaaca cgtcaggatt
     2341 gcggaaggga caatatgcca ccagtcaccc tgtgccagca cgggcgtcag ctcggcattt
     2401 ttggcaaata ccgcacaagc aatagaaaag agaaggtgac cttccgccgt catgataaaa
     2461 ctcactaaac tgtcgatacg tacagtatag gatttttata cagtgcacgg aagtggtgac
     2521 ggtgtaactc tttctcttaa attgacgatt aatccgtcag ttgaatgcaa attcagataa
     2581 agaaccaggg gcagcgagat cactgcccct ggtcattaac cgagaaggtc ttttgcggtg
     2641 agttctgtca gcgatgagag tttgacgttt gctaatacaa aacgtggatc attttgcgct
     2701 tctggcgcag gaacgacaat ggaacgcatg cgggctgctt tagaggcgat catgccattt
     2761 actgaatctt ccagcgctac gcaggtcagt gggtcaacgc ccagttttgc tgcgcagtcg
     2821 agatatactt gcggatgcgg cttgctgtaa ggcagttttt cagccgaggc gagggcatcg
     2881 aaactgtcgc gtaagtcaaa catggtcaac actttttcca gcatatgtag tggtgacgcg
     2941 gaggccagtc ccaccaataa accttgttct ttgcataacg caacggcttc gcgcacgcct
     3001 ggtaataatg gacgtgtctc ttcaaccagt gaaatggcac gggcaataac ccgttctact
     3061 acttcctgac ggcttggccc attccacggt tgccgggcgt accaaagatc gaccaccata
     3121 tcgatgcgta aacctaaggt gtccggcagc tcgttacgac gggagatatc cacccccagg
     3181 cttgccatca catccagttc ggctcgatcc cataaaggtt ctgagttgat aagtaatcca
     3241 tccatatcaa aaattgcagc aagaatctga cgcggggttg acataacaac ctctccttta
     3301 gagcattatg tgtaaggtat taagccgtgc ttatgattaa gcgcctatca tatcgccttt
     3361 aaagaatggg cgaaaacaga tatctacagg taaactaagt gaaacgacgc gtcttaatca
     3421 aggagaactc atgacgtatc aacaagctgg acgcattgct gttttgaaaa ggattttggg
     3481 ttgggtgatt tttatacctg cattgatctc tacattgatt tctttgctga aatttatgaa
     3541 tacccggcag gaaaaccagg aaggcattaa tgcggtcatg ctcgacttta ctcatgtcat
     3601 gatcgatatg atgcaggcga atacgccttt tttgaatctg ttctggtata actccccgac
     3661 gcccaatttt aatggtggcg tgaacgttat gttctgggtg attttcatcc tgatttttgt
     3721 cggactggcg ttgcaggatt ccggtgcccg gatgagtcgc caggcgcgct tcttgcggga
     3781 aggtgttgaa gatcaactca ttctggaaaa agccaaaggg gaagaggggc taacgcgtga
     3841 acaaattgag tcccgtattg ttgttccgca ccatactatt ttcctgcagt ttttctcact
     3901 atatatcctg ccggttattt gtattgctgc aggttatgtg ttcttttctc tgcttgggtt
     3961 tatttaatca aaaggccgca cggagcggcc attatcctca acccatatca tgctgctaat
     4021 aatctatcca atgactgctg agcaataacc aaatgctgac cgccaaataa tcttgcacga
     4081 tttaaaagcg tgtagagttg gtaaaccggt tgacgttcaa ggaaatcggc aggtagcggg
     4141 gatactgact gatagccgtc atagatttgt ggcggctgtt cagtatgcag cggtaacatc
     4201 gccaggtcgc actctcggtc accccagtag caggcagggt cgaaaatgta cgggccatcc
     4261 ggacccagcg cacagttgcc ggaccataaa tcgccgtgca acagagaagg ctgcggttga
     4321 tgcgaggcca gacgctgctg aatatgctcg acgatggcgt cgatattgcc gaaagcgatc
     4381 cctttctctg ctgccagttc caactgccag ccaatccgtt gttcagcaaa aaacgttgac
     4441 cagcgacgtt gccaggtgtt gggctgtgga gttgtggaga gcgagttatc aaaatcgagg
     4501 ccaaattgtg gttggtcact ccattgatgt aaacgcgcaa tttgctgacc aagaataaat
     4561 gcgctatgcg catccagcgg acgaggtggg agataatcca tcaccagaaa actgtagtca
     4621 cggtcagcgc ctactgccca aaccttaggc acggtgacgg ttttactacg cgacagtaac
     4681 tccagttggt cggcttcggc ggtaaaaccg ggaagcagtt ccctttcatc acatttgacg
     4741 aaaaagtcat gtcctgcata gcgcaaatgc catgcggcat ggacttctcc gccaggcagt
     4801 tcattacgca gttcgatttc gccttcacct aactgctcgc tcaaaagacg actgattgcc
     4861 tgccacatga tgtctctccc atgttgtctg ccagatcata aggttagcgc aagaatgcgg
     4921 tgtaaaaaca cgagctaacg cacaccacag actgattatt gcccaaaaac catttatttt
     4981 ctgttgctat tccattcctc ccaggtcgtg atggtgattt caggatcttt gccggttgca
     5041 ctttgggtaa gccccgaaac cagttctcta atttcttctt cactttgggt actgataagt
     5101 ccaaaagtgt tagtccccag ctcatggata tttccctcat catccgtcat ggtgagcaaa
     5161 aaaccgtcgc gagtcaggta attattcaac tcgttaattt cagtcaatgt atcctcatgc
     5221 aacatgacgg ttatgacata acggacaaga tcgccactgg ccatagttca cccctttgtt
     5281 atcacatgac ttttgagcat agtcggagaa acgcgttgcc gacaggttgg tggtgattcc
     5341 cccaatgctg ggggaatgtt tttgttagcg ggaaaggtaa gcgtaaattt tttgcgtatc
     5401 gtcatgggag cacagacgtg ttccctgatt gagtgtggct gcactccccg cagctacgcc
     5461 aaaacggacc atctcttcaa gagaggcatt ttctgccagt ttcagtgtca tcgcgccgac
     5521 catgctgtca ccagcgccaa cggtactctg gcttttcacc ggtggtggca ccacctgaat
     5581 gcagttttca ctatcaacac ccagcgctcc ttgtggacca agggaaacga caacccgttt
     5641 ggccttgccg ctattaacga tttcctgcgc ggctttgcgg acatcgtctg gctgggtgag
     5701 ttcgcgattc accagcgcac tgagttcttt ttggttaggc ttaaccaact cgatgttacc
     5761 aatcgccagt gctgcactta acgcttcgcc agaactgtcg atgatgcagc ggatcccttg
     5821 gttttgcgca gcggaaatca gttgggttaa tttttccagc ttcacacctg gcggcaggct
     5881 tccgcttatg accaggatgg ccccggattc aatttccaga acttgctctt caagctggcg
     5941 aaactcatct tcatttaatg ccgcgcctgg cataacaaaa cgatactgct caccgcttgc
     6001 ttccacatgt acgtgtaaat tctgccgggt ccagtctttg gcttctacag tagcgacggg
     6061 gacattttca tccgccaaca gagaaaccag gtgttcgccg gtcgcgccac ccgccgggaa
     6121 gatcgctgtg gcactgcctc caagatgggc aatggcgcgg gcgacgttga tgccgccgcc
     6181 cccgggttcg aacaccggtg cggtacagcg cagttttcct tcgggataaa tttgcggggt
     6241 aattgttgcg ctatcgagag agggcgcaag tgtcaacgta tagatacgta ccatcatttc
     6301 ctcctatagg ctgatttcag tctggcaccg aatcagccga aagtgaagaa attaacaata
     6361 tgatttatta gagctttatt taaaattttg cagataaata tatataaata aaaatctgtt
     6421 ttcgccacat gcttattca
//
LOCUS       DAZFRN010000225         6430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFRN010000225 DAZFRN010000000
VERSION     DAZFRN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.227"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            34..2295
                     /locus_tag="ACHKUB_004713"
     CDS             34..2295
                     /locus_tag="ACHKUB_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6657624.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSVNMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGHKPACDSRVICVPQGSYDIDD
                     KVGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLVVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            2364..3539
                     /gene="senB"
                     /locus_tag="ACHKUB_004714"
     CDS             2364..3539
                     /gene="senB"
                     /locus_tag="ACHKUB_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFO6657625.1"
                     /translation="MNIFTLSKTPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSGDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(4169..4627)
                     /locus_tag="ACHKUB_004715"
                     /pseudo
     CDS             complement(4169..4627)
                     /locus_tag="ACHKUB_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612075.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(5067..5453)
                     /locus_tag="ACHKUB_004716"
     CDS             complement(5067..5453)
                     /locus_tag="ACHKUB_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO6657626.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGIHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(5450..5674)
                     /locus_tag="ACHKUB_004717"
     CDS             complement(5450..5674)
                     /locus_tag="ACHKUB_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO6657627.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            5740..6095
                     /locus_tag="ACHKUB_004718"
                     /pseudo
     CDS             5740..6095
                     /locus_tag="ACHKUB_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6328..6381)
                     /locus_tag="ACHKUB_004719"
                     /pseudo
     CDS             complement(6328..6381)
                     /locus_tag="ACHKUB_004719"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
BASE COUNT         1819 a         1506 c         1481 g         1624 t
ORIGIN      
        1 ttatatttat taatattata tctaaggaaa tttatgaacg ttataaaact ggctatcggc
       61 tcaggcatat tattgctcag ctgcggtgct tactcacaat ccatcagtga aaaaactaat
      121 tccgacaaaa aaggagcggc agaattcagt ccgctcagcg tttctgtcgg gaagacgacc
      181 agtgagcagg aagctctcga gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac
      241 ctgcaatcac ttgacgcaac agtgcgtagt atgcccggta cttatactca aatagatcct
      301 ggtcagggag caatcagtgt gaatattcga ggcatgagcg gatttggtcg tgtaaacact
      361 atggtcgatg gtattaccca gagtttttac ggaacctcta cctccggaac aacgacgcat
      421 ggttcaacta acaatatggc tggcgtactt atagatccta acttactggt agcagttgat
      481 gttacacgcg gtgacagcag tggctctgaa gggatcaacg cccttgccgg tagtgtaaat
      541 atgcgtacta ttggcgttga cgatgtaata tttaacggta atacatatgg ccttcgttca
      601 cgtttctctg tcggtagtaa tgggctggga cgcagcggaa tgatcgccct tggtggaaaa
      661 agcgacgctt ttacggatac gggaagcatt ggcgttatgg ctgctgtgag cggcagttct
      721 gtgtactcta atttctcaaa tggttctgga attaacagca aagagtttgg ttatgataaa
      781 tatatgaagc agaaccccaa atcccaactg tataaaatgg atatcagacc agacgaattt
      841 aacagcttcg aactttccgc tcgaacctat gaaaataaat ttacacgtcg tgatataacc
      901 agtgacgact attacattaa atatcattac accccttttt ctgaattaat tgactttaac
      961 gtaacggcca gtaccagtcg cggtaatcaa aagtatcgtg atggctcgct gtatactttc
     1021 tacaaaacct cagcgcaaaa tcgttctgac gcgctggata tcaacaatac cagccggttc
     1081 actgtcgcgg acaatgacct ggagtttatg ctgggcagca aactgatgcg tacccgctat
     1141 gaccggacca ttcactcagc ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac
     1201 aatccgttcg caccctccgg ccagcaggat atttcagcgc tgtataccgg gctgaaggtt
     1261 acgcgcggca tctgggaggc agatttcaat ctcaactaca cacgtaacag gatcacaggg
     1321 cacaagcccg cctgcgattc acgcgttatc tgcgtgccac agggtagcta cgatattgac
     1381 gataaagtgg gtggcttcaa cccttcagtt cagctttctg ctcaggtaac accatggctt
     1441 cagccgttca ttggctacag caaatccatg cgcgccccga acatccagga gatgttcttc
     1501 tctaattcag gaggcgcatc catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg
     1561 caggcgggtt ttaacattga taccagagat ttagtggtcg aacaggatgc cctgcgcttt
     1621 aaggctctgg cgtaccgcag caggatccag aactacatct acagcgagtc ttatctggtt
     1681 tgttctggag gtcgtaaatg cagtatggct gaggtgattg gcaatgactg ggagggcatt
     1741 agcgatgaat acagcgacaa tatgtacatc tacgttaact cggcaagcga cgttatcgca
     1801 aagggcttcg aactggagat ggattatgat gcaggttttg cttttggccg actctctttc
     1861 agccagcagc aaacagacca gccaacctcc atcgccagca cctactttgg cgcaggggat
     1921 atgaccgaac tgcccagaaa atacatgacg ctggatactg gtgttcgctt cttcgataac
     1981 gcgttgaccc tgggcactat cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat
     2041 tttgagcagg acgaacatac cggcgcaata atcaaacagg atttgccgca gatcccaacg
     2101 attatcgatc tctatggtac ttacgagtac aaccgcaacc tgacactgaa actttcggta
     2161 caaaacctga tgaacagaga ttattcggag gcgctgaata agctcaacat gatgccaggt
     2221 cttggtgacg agacccaccc agccaattcc gcgcgtggca gaacatggat atttggcggg
     2281 gacattcgtt tctgatgaca atttccgcta ggttcagaca atacgttttc tctcttatgt
     2341 caattttatt gcaggaacga aaaatgaata ttttcacttt atccaaaaca ccgctatacc
     2401 tgttaatttc actattttta cccacgatgg ccatggctat cgatccacct gaacgcgaac
     2461 tttcgcgatt tgccctgaaa acgaattacc ttcagtcccc tgatgaaggc gtctatgaac
     2521 tggcgtttga taatgccagt aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg
     2581 aagccaataa aggctatctg tattcgttta attcagattc gctgaaagtc gaaaataaat
     2641 acacgatgcc ataccgggca ttttcgctgg cgataaatca ggataaacat cagctctata
     2701 tcggacacac ccagtcagcg tccctgcgta tcagtatgtt tgacacccca accggcaaac
     2761 tggtaagaac cagcgacagg ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg
     2821 agcatttgcg ccatatggtt tacagccagg attccgatac cctgtttgtg agttatagca
     2881 atatgctgaa aacggccgag ggcatgaagc ctctgcataa gctgttaatg ctcgacggga
     2941 cgacgcttgc cttaaaaggc gaggttaagg atgcttacaa aggtacagcg tatggtctga
     3001 cgatggatga aaaaacacag aaaatctacg ttggcggaag agattacatc aacgaaattg
     3061 atgcgaaaaa tcagacgctg ctgcgtacca tcccgttgaa agatccgaga ccacaaatca
     3121 caagtgtgca gaatctggcg gtggactccg cttctgaccg tgcctttgtg gtggtattcg
     3181 accatgacga tcgttccggt acaaaagatg gactctatat ttttgactta cgcgacggta
     3241 aacagcttgg ctatgtgcac acaggagccg gagctaacgc ggtgaaatac aatccgaaat
     3301 ataacgaact gtatgtcacc aacttcacta gcggcaccat cagcgtagtg gatgccacca
     3361 aatacagcat cacccgtgaa tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg
     3421 gtgatatgga taccctttac attggcatca aagaaggctt taaccgcgat tgggatcctg
     3481 atgtgtttgt ggaaggagct aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat
     3541 cgggggaaaa catgggataa aaactccgtt attccactaa actggccgga ttaggaaaag
     3601 caaacatctc tgtcctgccg gtacgcctgt tacgccgctc catgaggctg aggaatctcc
     3661 agaaaacgag acaggcacag cattgtacga tcaactgatt tgtgccaata attcaatgaa
     3721 atcacagcta tgcctgttca tggggttcta ggccggaacc ccagattttt ccgattgttt
     3781 gttttctaac tgattattca gtagttttag agggctgacg ctcagtggaa cgaaaactca
     3841 cgttaagagg ttttgactat gataaaagtc aaagtagggg tgagtttgga tatggaaatt
     3901 attgtactat aagggcttcc ccgaacagtc aacgtcggta tttatggttt gtctgcacta
     3961 cagttaaaaa taaaggggag agactgcggt actataaacg gtcataataa atcgttatcg
     4021 attcggaccc tgatgaaaga cgcgcgtgag gctcttgttc gttaagcgga tacgtatatc
     4081 ctgtacaata atcagatata cctcacgggg tctaaccctt tatcatcaac gagttgcagg
     4141 tcagatagtc atacggttgc tgcgtgcttt agctggctga atacagtctg aaatcatcac
     4201 cgtaatgcaa tgatgcccag cacagccttg catttttggc ggcgatggcc acaacagcgc
     4261 gccagtatcc tctgcgttcg accagtgaac agacccaacg actgaatgag tcagtccttt
     4321 tctcagcgcc aatcagtact gaacgagcac cctgaaccag aagcgttcgc agatacgaat
     4381 caccggcttt tgttatcctg cctagcttcg actttccgcc gctgctgtat tgtgatggag
     4441 tcagtcccag ccaggccgcc agttgccgtc catttttaaa atcatgtgca ttaccgatac
     4501 aggcaaccag cgcacaggca gttgtgggac caattccttt tagctccatc agccgctgac
     4561 tgcggtgatc tgttcttgcc atacgggaca aaattttgtc atattcagtg atgttagctt
     4621 caatgcgggg tcagtttgga gatcgtaaaa ttttgttcgt taagcacatt atttaaccaa
     4681 tagatatgcg taacggattg attttgcgtc aacggctatc gcttttaagt tatcgccatt
     4741 gaggtattgc atttaggtta tccgatagag gtcaaatcct ctgaaaacgc cgtatagcac
     4801 gggagtacac cctaccgcaa tagcgcaacc gtacaatatt gtacgtttcc caaacgcagc
     4861 ctaggaagca cgtcgtcagt attggagaaa acttacaaac gctcgccgca gccccagcga
     4921 ccgaacgtta gtgagcgagt gggcgaggaa gcggaaaagt gtcaggtctg aattgagcac
     4981 atacgccaga aattatatag caaaaagata tatgattttt acattgctca gtagattgta
     5041 aaatgctgct gattggatat ctatatctaa tttaaaataa tggctttaag atggtcagaa
     5101 accatcttcc agttatattc tcctgttgca gcattcaaag cgataatttg catttcatca
     5161 aacgaaatac gcgaactaat aatatgtata ccattccgtt ttaaaaaaac ctgtgcaaca
     5221 aacaaggctg ttcgcttatt tgcatcgttg aaaatgtgac cacgagcaat agcaactaaa
     5281 tacacggcag ccagatccat aatatctgtc acaccttcat aatgatacat gttcaaaaca
     5341 cgatgaatga tcgcgtcagc gcgaccgggt tctggcatac ccgccacgcc accatcacgc
     5401 gaaatcaagc ggtcatgaaa ctcaatcatt tcctgtgaac taacgaattt catcgcttag
     5461 aaagctccgt ataggccgtc ccatgacttt gcatgatctc agcaaactca gcatcaagag
     5521 ccgctttacg gtatcgttca aaagcgtctt tatcaaccag tacagccgcg ccacctttac
     5581 ggcgctgaat ctcaacaggc tgattttcat caaccgccag ctcgagcaac gaggccaggt
     5641 tatcgcgagc atggctatag ttacatgtaa acatagtgct tcctctttga gttgtacaat
     5701 taaattgtac aacagttatc ttcaatatga aatcaccgtt accgctggcg catcctgacc
     5761 caacttaacc ggggagaaag tcgccatgcc gttgccagag ctatctgtca cggtcagaaa
     5821 ggtgagataa gaaagcgata taccgatggt caagaagatc aactgggcgc actggggctg
     5881 gtgactaacg ccgtcgtatt atggaacact atttatatgc aggctgccct ggaccatttc
     5941 agggcacagg gggaaatcgt gaatgatgaa gatatcgcac gcctctcacc actttgccac
     6001 ggacacatca atatgctcgg ccatattcct tcacgctggc agaactggtg actaaagggc
     6061 aaaaagaggc atcggaggaa gaaaacattg cttaacgtga gttttcgttc cactgagcgc
     6121 cagcccccta attctctata tccaaactga ccccaatagg tgacgggtta tgtgacgaaa
     6181 tctgatgtag aaatcgttgt ttcagtgaca gtcactcaat cggtcattta tgtgacaccc
     6241 cacgccgtta ctggtcgcgg aaaaatccag cggaaaaatc caggtttccg gcttgggacc
     6301 ccatctcaag agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagaaga
     6361 cgaagatgac gcaacggttc ctcattgtac atcacgaata tagtttgctt gacatcctga
     6421 caagaataat
//
LOCUS       DAZFRN010000226         6426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFRN010000226 DAZFRN010000000
VERSION     DAZFRN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.228"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            18..983
                     /gene="iaaA"
                     /locus_tag="ACHKUB_004720"
     CDS             18..983
                     /gene="iaaA"
                     /locus_tag="ACHKUB_004720"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6657628.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            970..2841
                     /gene="gsiA"
                     /locus_tag="ACHKUB_004721"
     CDS             970..2841
                     /gene="gsiA"
                     /locus_tag="ACHKUB_004721"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO6657629.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            2861..4399
                     /gene="gsiB"
                     /locus_tag="ACHKUB_004722"
     CDS             2861..4399
                     /gene="gsiB"
                     /locus_tag="ACHKUB_004722"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO6657630.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            4417..5337
                     /gene="gsiC"
                     /locus_tag="ACHKUB_004723"
     CDS             4417..5337
                     /gene="gsiC"
                     /locus_tag="ACHKUB_004723"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO6657631.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            5340..6251
                     /gene="gsiD"
                     /locus_tag="ACHKUB_004724"
     CDS             5340..6251
                     /gene="gsiD"
                     /locus_tag="ACHKUB_004724"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO6657632.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         1416 a         1542 c         1886 g         1582 t
ORIGIN      
        1 cttaatggac gaatactatg ggcaaagcag tcattgcaat tcatggtggc gcaggtgcaa
       61 ttagccgcgc gcagatgagt ctgcaacagg aattacgcta cattgaggcg ttgtctgcca
      121 ttgttgaaac cgggcagaaa atgctggaag cgggcgaaag tgcgctggat gtggtgacgg
      181 aagcggtgcg tctgctggaa gagtgtccac tgtttaacgc cggaattggc gctgtcttta
      241 cgcgtgatga aacccatgaa ctggacgcct gtgtgatgga tggtaacacc ctgaaagccg
      301 gtgcggtggc gggcgttagt catctgcgta atccggttct tgccgcccgg ctggtgatgg
      361 agcaaagccc gcatgtgatg atgattggcg aaggggcaga aaattttgcg tttgctcgtg
      421 gcatggagcg cgtctcgccg gagattttct ccacgccttt gcgttatgaa caactactgg
      481 cagcgcgcaa ggaaggggca accgtcctcg accatagcgg tgcgccactg gatgaaaaac
      541 agaaaatggg caccgtgggg gccgtggcgt tggatttaga cggcaatttg gcggcagcca
      601 cgtccacagg cggaatgacc aataaattac ccggacgagt tggcgatagt cccttagtgg
      661 gtgccggatg ctacgccaat aacgccagtg tggcggtttc ttgtaccggc acgggcgaag
      721 tcttcatccg cgcgctggcg gcatatgaca tcgccgcgtt aatggattac ggcggattaa
      781 gtctcgcgga agcctgcgag cgggtagtaa tggaaaaact ccctgcgctt ggcggtagcg
      841 gtggcttaat cgctatcgac catgaaggga atgtcgcgct accgtttaac accgaaggaa
      901 tgtatcgcgc ctggggctac gcaggcgata cgccaaccac cggtatctac cgtgaaaaag
      961 gggacaccgt tgccacacag tgatgaactt gatgccggta atgtgctggc ggttgaaaat
     1021 ctgaatattg cctttatgca ggaccagcag aaaatagctg cggtccgcaa tctctctttt
     1081 agtctgcaac gcggtgagac gctggcaatt gttggcgaat ccggctccgg taagtcagtg
     1141 actgcgttgg cattgatgcg cctgttggaa caggcgggcg gtttagtaca gtgcgataaa
     1201 atgctgttgc agcggcgcag tcgcgaagtg attgaactta gcgagcagag cgctgcacaa
     1261 atgcgccatg ttcgcggtgc ggatatggcg atgatatttc aggagccgat gacatcgctg
     1321 aacccggtat ttactgtggg tgaacagatt gccgaatcaa ttcgtctgca tcagaacgcc
     1381 agtcgtgaag aagcgatggt cgaggcgaag cggatgctgg atcaggtacg cattcctgag
     1441 gcacaaacca ttctttcacg ttatccgcat caactctctg gcgggatgcg ccagcgagtg
     1501 atgattgcga tggcgctgtc atgccgcccg gcggtgctga ttgccgatga gccaaccacc
     1561 gcgctggatg tcactattca ggcgcagatc ctgcaattaa tcaaagtatt gcaaaaagag
     1621 atgtcgatgg gcgttatctt tatcactcac gatatgggcg tggtggcaga gattgccgat
     1681 cgggtactgg tgatgtatca gggcgaggcg gtggaaacgg gtaccgtcga acagattttt
     1741 catgcaccgc aacatcctta cacccgtgcg ctgttagctg ctgttccgca acttggtgcg
     1801 atgaaagggt tagattatcc ccgacgtttc ccgttgatat cgcttgaaca tccagcgaaa
     1861 caggcccccc ccatcgagca gaaaacggtg gtggatggcg aacctgtttt acgagtgcgt
     1921 aatcttgtca cccgtttccc tttgcgcagc ggtttgttga atcgcgtaac gcgggaagtg
     1981 catgccgttg agaaagtcag ttttgatctc tggcctggcg aaacgctatc gctggtgggc
     2041 gagtctggca gcggtaaatc cactaccggg cgggcgttgc tgcgcctggt cgaatcgcag
     2101 ggcggcgaaa ttatctttaa cggtcagaga atcgatacct tgtcacccgg caaacttcag
     2161 gcattgcgcc gggatattca gtttattttt caggaccctt acgcttcgct ggacccacgt
     2221 cagaccatcg gtgattcgat tatcgaaccg ctgcgtgtac acggtttatt gccaggtaaa
     2281 gacgcggctg cacgcgttgc gtggttgctg gagcgcgtgg gcctgttacc tgaacatgcc
     2341 tggcgttacc cgcatgagtt ttccggcggt cagcgccagc gcatctgcat tgctcgcgcg
     2401 ttggcattga atccaaaagt gatcattgcc gacgaagccg tttcggcgct ggatgtttct
     2461 attcgcgggc agattatcaa cttgttgctc gatctccagc gtgatttcgg cattgcgtat
     2521 ctgtttatct cccacgatat ggccgtggta gagcggatta gtcatcgtgt ggcggtgatg
     2581 tatctcgggc aaattgttga aattggtcca cggcgcgcgg tcttcgaaaa cccgcagcat
     2641 ccttatacgc gtaaattact ggcggcagtt ccggtcgctg aaccgtcccg acaacgaccg
     2701 cagcgtgtac tgctgtcgga cgatcttccc agcaatattc atctgcgtgg cgaagaggtg
     2761 gcagccgtct cgttgcaatg cgtcgggccg gggcattacg tcgcacaacc acaatcagaa
     2821 tacgcattca tgcgtagata acattcaggc ggagaataaa atggcaagag ctgtacaccg
     2881 tagtgggtta gtggcgctgg gcattgcgac agcgttgatg gcatcttgtg cattcgctgc
     2941 caaagatgtg gtggtggcgg taggatcgaa tttcaccacg ctcgatccgt atgacgcgaa
     3001 tgacacgtta tctcaggccg tagcgaaatc gttttaccag gggctgttcg gtctggataa
     3061 agagatgaaa ctgaaaaacg tgctggcgga gagttatacc gtttccgatg acggcattac
     3121 ttacaccgtg aaattgcggg aaggcattaa attccaggat ggcaccgatt tcaacgccgc
     3181 ggcggtgaaa gcgaatctgg accgggccag cgatccggcg aatcatctta aacgctataa
     3241 cctgtataag aatattgcca aaacggaggc gatcgatcca acaacagtaa agattaccct
     3301 caaacagccg ttctcagcgt ttattaatat tcttgcccat ccggcgaccg cgatgatttc
     3361 accggcagcg ctggaaaaat atggcaagga gattggtttt catccggtgg gaaccggacc
     3421 gtatgaactg gatacctgga atcagaccga ttttgtgaag gtgaaaaaat tcgcgggtta
     3481 ctggcagcca ggattgccca aactggacag cataacctgg cgtccggtgg cggataacaa
     3541 cacccgcgcg gcaatgctgc aaaccggtga agcgcagttt gctttcccca ttccctacga
     3601 gcaggccaca ctgctggaga aaaacaaaaa tatcgagttg atggccagtc cgtcaattat
     3661 gcagcgttat atcagtatga acgtgacgca aaagccgttc gataacccga aggtccgtga
     3721 ggcgctgaat tacgccatta accgtccggc gctggtgaaa gttgcctttg cgggctatgc
     3781 aacgccagct actggtgtgg taccgccaag tatcgcctac gcgcaaagtt ataaaccgtg
     3841 gccttacgat ccagtgaaag cgcgcgaatt actgaaagag gcgggatatc ccaacggttt
     3901 cagtaccacg ctgtggtcgt cacataacca cagcaccgcg cagaaagtgc tgcaatttac
     3961 ccagcagcag ttagcgcagg tcgggattaa agcccaggtg actgcgatgg atgccggaca
     4021 gcgggcggca gaagttgaag gtaaagggca aaaagagagc ggcgtgcgga tgttctacac
     4081 tggctggtcg gcttcaaccg gcgaagccga ctgggcacta tcgccgttgt ttgcctcgca
     4141 aaactggcca ccgacgctgt ttaataccgc gttttacagc aataaacagg tggatgactt
     4201 cctggctcag gcactgaaaa ctaatgatcc ggcggaaaag acccgcttgt ataaggcggc
     4261 gcaggatatc atctggcaag agtcgccgtg gatcccgctg gtggtagaaa aactggtgtc
     4321 ggcacacagt aaaaacctga ccggtttttg gatcatgcca gacaccggct tcagctttga
     4381 agacgcggat ttgcaataag caacgcaggg agtggaatgc ttaattacgt tatcaaacga
     4441 ttactggggt tgattccgac gctgtttatc gtctcggtgc tggtgttttt atttgtgcat
     4501 atgctgcccg gcgatccggc gcgattgatt gccgggcccg aagctgatgc gcaggttata
     4561 gaactggtgc gtcagcagct ggggttggat cagccgctgt atcaccagtt ctggcactat
     4621 atcagcaatg ctgtgcaggg ggattttggc ctgtcgatgg tgtcgcgtcg tccggttgcc
     4681 gatgagattg ccagccgctt tatgccaacg ctgtggctga ccataaccag tatggtctgg
     4741 gcggtgatat ttggtatggc ggcgggaatt atcgccgccg tctggcgtaa tcgttggccg
     4801 gatcgattga gtatgaccat tgcggtgtcg gggatctcgt ttccggcatt tgcgctgggg
     4861 atgcttttaa ttcaggtatt ctccgttgaa ctgggctggc tgcctaccgt gggagcagac
     4921 agttggcagc actacatttt accctccctg acgctcggcg cggcagtggc agccgtgatg
     4981 gcgcgcttta cccgcgcgtc gtttgtcgat gttttaagcg aagattatat gcgtaccgcg
     5041 agggcgaaag gggtgagcga aacctgggtt gtcctcaaac acgggctacg taacgcgatg
     5101 atcccggtag tgaccatgat gggcttacag tttggctttt tgctcggtgg ttctatcgtg
     5161 gtggagaaag ttttcaactg gcccggactt ggacgcttac tcgttgactc cgtagaaatg
     5221 cgcgattacc cggtgattca ggcggaaatt ctgcttttct cgctggaatt tattcttatc
     5281 aacttagtgg tggatgtgct ttacgccgcc attaacccgg ctatcaggta caagtaagga
     5341 tgcgactatt taactggcga cgtcaggcgg tgttaaacgc catgccactg gtcaaacctg
     5401 accaggtgcg tacaccgtgg catgaattct ggcgacgatt tcgccgtcag catatggcga
     5461 tgaccgccgc attattcgtt attttattga ttgtggtggc catttttgca cgctggatcg
     5521 ctccctatga cgccgaaaat tattttgatt atgacaatct gaataacgga ccttctttgc
     5581 agcactggtt tggcgtcgat tcactggggc gcgacatttt cagccgtgtc ctggttggtg
     5641 cgcaaatctc gctggcggcg ggcgtgtttg ccgtgtttat cggtgcggcg atagggacgt
     5701 tgctgggctt gctggctgga tattatgaag gctggtggga tcggctgatc atgcgcattt
     5761 gcgatgtgct gtttgccttc ccgggtattt tactggcgat cgctgttgtt gcggtgttgg
     5821 gaagcggcat tgctaacgtg attatcgcag tcgccatttt ttccatcccc gcgtttgccc
     5881 gcctggtgcg cggcaacacg ctggtgttga aacagcaaac ctttattgag tcagcacgca
     5941 gtattggtgc cagcgatatg accattttat tgcgtcatat cttgcctggg accgtctcgt
     6001 ctatcgtggt gtttttcacc atgcgcattg gcacctcgat tatctctgcc gccagcctgt
     6061 catttctcgg cctcggtgcg cagccgccga caccagagtg gggagcaatg ctcaatgagg
     6121 cacgagcgga tatggttatc gcgccgcatg tcgctgtttt tccggccctg gctatttttc
     6181 tgaccgtact ggcgttcaat ttgttgggcg atggtttacg cgatgcgctg gatccgaaaa
     6241 ttaaaggata gttacgtttg aatattgctt gaaagggtaa tcacctcaca ggaaattatt
     6301 gccctaagca agtgttgtaa ctttctgctg attttgtaga atcgggtaat ttggttaaaa
     6361 agccgcagca agggactatt tttgcagcgg cacagcgttc agatagttat tttgttaaat
     6421 gtatta
//
LOCUS       DAZFRN010000227         6419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFRN010000227 DAZFRN010000000
VERSION     DAZFRN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.229"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..251)
                     /locus_tag="ACHKUB_004725"
     CDS             complement(<1..251)
                     /locus_tag="ACHKUB_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFO6657633.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRM"
     gene            681..962
                     /gene="yjcB"
                     /locus_tag="ACHKUB_004726"
     CDS             681..962
                     /gene="yjcB"
                     /locus_tag="ACHKUB_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO6657634.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(1061..1597)
                     /gene="ssb1"
                     /locus_tag="ACHKUB_004727"
     CDS             complement(1061..1597)
                     /gene="ssb1"
                     /locus_tag="ACHKUB_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO6657635.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1851..4673
                     /gene="uvrA"
                     /locus_tag="ACHKUB_004728"
     CDS             1851..4673
                     /gene="uvrA"
                     /locus_tag="ACHKUB_004728"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO6657636.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4708..5064)
                     /gene="yjbR"
                     /locus_tag="ACHKUB_004729"
     CDS             complement(4708..5064)
                     /gene="yjbR"
                     /locus_tag="ACHKUB_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6657637.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(5068..5484)
                     /gene="yjbQ"
                     /locus_tag="ACHKUB_004730"
     CDS             complement(5068..5484)
                     /gene="yjbQ"
                     /locus_tag="ACHKUB_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO6657638.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5595..6308)
                     /gene="aphA"
                     /locus_tag="ACHKUB_004731"
     CDS             complement(5595..6308)
                     /gene="aphA"
                     /locus_tag="ACHKUB_004731"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO6657639.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1528 a         1673 c         1671 g         1547 t
ORIGIN      
        1 aacattcgct tttgatgggc tggagtgcaa acttgcccct gatagcgttc agctgcatcg
       61 ctcactaaat ctgcctggcg aatgaccagc tcggatttat ccagtgccag ttgagcaaag
      121 gttcggagct gattattcac ttctgatttt gcccaaagga aggcaatcca tagcgaaaga
      181 atgatgggaa agagaactaa aaagataatg cccggcaacg ccagtaattg gtgtcgtgca
      241 cgatgactca ttgctatcaa tttcctgcct gtttataccc ggccattgca aggtgaaaac
      301 cctgggtacc gacgtcttta gataaatctt acggtataaa tagcactttt gcctgatttg
      361 aaattgatac ctttctaatc gtcacctgga tgaaaataat tcattgatat tcaaaattaa
      421 tttcctacag gaatgcagag gcggcgggaa gatatgccct taatgccact gaaaaaccac
      481 ataatctgta cagcaataaa agtcacggcc taatatggct aacaaacaat caattgtgat
      541 atagttcaca aaattaatga aacaaacaga gtgtttcatt tttgtgttct gcaagccccg
      601 cgatagccag agtgtaccac gcctcccgtg aacaacgccg cgctgtccag ggcatcggct
      661 cttttcagag gaattgtttg atggctaccc tcaccactgg cgtggttctt cttcgctggc
      721 aacttcttag tgccgtaatg atgtttctgg ccagcacact caacatccgt tttcgtcggt
      781 ctgattatgt cgggcttgcg gtgatcagca gcggtctggg cgttgtttcc gcctgttggt
      841 tcgcaatggg gttgcttggc atcacaatgg cggacatcac cgctatctgg cacaacatcg
      901 agtcggtgat gatagaagag atgaatcaga caccgccaca atggccaatg attttgacct
      961 gatacgtaga agcctccata acggaggctt cttttttatg gctggcagat gctttaatca
     1021 tccaccttaa aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat
     1081 caaagtccat cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact
     1141 gcgcgccgcc gctgaactga ttgccaccct gcggctgctg aggctgaccc caaccgccct
     1201 gcggctgacc accaccgata ttgccacctg ccggagcgcc accaccctga cgaccaccca
     1261 gcatctgcat ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg
     1321 attgatcggt ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac
     1381 gcagatattc gctggccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag
     1441 tctgttcttt catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa
     1501 tgttggcaac tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac
     1561 caacgagaat aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc
     1621 gttctgaata agtgtaaacg cgcgattgta ccattaccaa tagcgctttt actatgttgt
     1681 gacctcggtt ccgggaaaca aacctggcca gacattgtta cacaacactc cgggtaatgc
     1741 attccaatac tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc
     1801 cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga
     1861 tcgaagttcg gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg
     1921 acaagctcat tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca
     1981 ccttatatgc cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt
     2041 ttctgtcact gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct
     2101 caattgagca gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa
     2161 tccacgacta tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg
     2221 acgtcccgct ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg
     2281 aaggcaagcg tctgatgctg ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca
     2341 aaacgctgga gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct
     2401 gcgatctttc cgatccgccg aaactggaac tgcaaaagaa acacactatt gaagtggtgg
     2461 ttgatcgctt caaggtgcgt gacgatctta cccaacgtct tgcggagtcg tttgaaaccg
     2521 cgctggagct ttccggtggt acagcggtag tggcggatat ggacgacccg aaagcggaag
     2581 agctgctgtt ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg
     2641 agccgcgact gttttcgttt aacaacccgg caggtgcctg cccgacctgt gacggccttg
     2701 gcgtacagca atatttcgat cctgaccgcg tgatccaaaa ccccgagctg tcactggctg
     2761 gcggtgcgat ccgtggctgg gatcgccgca acttctatta tttccagatg ctgaaatcgc
     2821 tggcagatca ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc
     2881 ataaagtggt gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc
     2941 gtggcgatac ctccatccgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc
     3001 gttataaaga gacagaatcc agtgcggtac gtgaagaatt agccaagttt atcagcaatc
     3061 gcccatgcgc cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg
     3121 agaatacgcc gctgcctgct atctccgaca tgagcatcgg tcatgcgatg gaattcttca
     3181 acaatctcaa actcgctggt caacgggcga agattgcgga aaaaatcctt aaagagatcg
     3241 gcgatcgttt gaaattcctc gttaacgtcg gcctgaatta cctgacgctt tcccgctcgg
     3301 cagaaacgct ttccggcggt gaagcccagc gtatccgtct ggcgagccag attggtgcgg
     3361 gcctggttgg cgttatgtac gtgctggacg agccatctat cggcctgcac cagcgcgata
     3421 acgagcgcct gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg
     3481 tggagcacga cgaagacgca attcgcgccg ctgaccatgt gatcgacatt ggcccgggcg
     3541 caggtgttca cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatt atggcggtgc
     3601 cggagtcgtt gaccgggcag tacatgagcg gcaaacgcaa gattgaagtg ccgaagaaac
     3661 gcgttccggc gaatccggaa aaagtgctga agctgacagg cgcacgcggc aacaacctga
     3721 aggacgtgac gctgacgctg ccggtgggtc tgtttacctg catcaccggg gtttcaggtt
     3781 ccggtaaatc gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg
     3841 gggcgaccat cgccgaacca gcaccgtatc gcgatattca ggggctggag catttcgata
     3901 aagtgatcga tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct
     3961 ataccggcgt gtttacgcct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc
     4021 gtggttatac gccaggacgt ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc
     4081 agggcgacgg tgtgatcaaa gtagagatgc acttcctgcc ggatatctac gtgccgtgcg
     4141 atcagtgtaa aggtaaacgc tataaccgtg aaacgctgga aattaagtac aaaggcaaaa
     4201 ccatccacga agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgccgtac
     4261 ctgcactggc gcgtaagctg caaacgttga tggacgttgg cctgacgtac attcgcctgg
     4321 ggcagtccgc aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc
     4381 tgtcaaaacg cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc
     4441 acttcgccga tattcagcaa ctgctcgacg tactgcataa actgcgcgat cagggcaaca
     4501 ccattgtggt gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc
     4561 tgggaccaga aggcggcagt ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg
     4621 tcgcggagtg cgaagcttcg catacggcac gcttcctcaa gccgatgctg taatcgttaa
     4681 ggccgctttc tgagcggcct tttcctttca gagttgtacc agcaatttac gcttttcttc
     4741 cggtagtaaa ttcactgcct gctgataaga cgcatccacc agataataga tttgcgaatc
     4801 cggcagcgaa ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggctagg
     4861 acgcacatcg ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga
     4921 aacagctggg cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat
     4981 ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg cctggttttg ccatgcaata
     5041 ttgtagcaac tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg
     5101 cgatccgccg tggatgcgat gttcccccag ccagatgcct tgccaggtgc cggtctgaat
     5161 acgcccttta tgtaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga
     5221 aggcatatcg tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg
     5281 gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag
     5341 agaggcggag gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc
     5401 agccagctga ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag
     5461 cgtgagcgtc ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg
     5521 cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagcgtcac
     5581 gctctcccgc tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct
     5641 tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg
     5701 acatcgcgtg cggcggtaat atcgttatcg gaatcgccat aaaaaattcg gatattttta
     5761 tcctgcagcc attgcgattt tgtattttgc cctggtttat cgcccgcaaa gatcaccgga
     5821 ttcatgttgg tgacaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt
     5881 ttcgtcgggc tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca
     5941 atcagctggc gagcgacctc ttttggaatg ctgaattcat cccagccatt gttcattttt
     6001 tcccagaaca caggattttt cagataatct tcgctttctg gtgagaaggt ttttttgccg
     6061 cgccagaagc ccgggctgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt
     6121 ggcggacgcc ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aattggtgcc
     6181 tgttcagcaa gcctggcaac gttagtccca gggttaagcg gtgaaggaga tgaggccagg
     6241 gcaacagcgg aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc
     6301 ttgcgcatat ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg
     6361 caaaaaccat ctgaccataa cgccagctgc cggggaaagg aaggattatc tgcggtttt
//
LOCUS       DAZFRN010000228         6416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFRN010000228 DAZFRN010000000
VERSION     DAZFRN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.230"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            53..310
                     /locus_tag="ACHKUB_004732"
     CDS             53..310
                     /locus_tag="ACHKUB_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657640.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDW"
     gene            310..900
                     /locus_tag="ACHKUB_004733"
     CDS             310..900
                     /locus_tag="ACHKUB_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657641.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNREG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(918..1265)
                     /locus_tag="ACHKUB_004734"
     CDS             complement(918..1265)
                     /locus_tag="ACHKUB_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO6657642.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLLPLPF
                     MPFWQVTVLMGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            1394..1729
                     /locus_tag="ACHKUB_004735"
     CDS             1394..1729
                     /locus_tag="ACHKUB_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO6657643.1"
                     /translation="MNRKYYFNNMWWGWFMGGYTLYMSWDYEFKYRLLFWCISLCGMV
                     LYPVARWCIENTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLTLIYILS
                     VIIKRLSVR"
     gene            complement(1751..2853)
                     /locus_tag="ACHKUB_004736"
                     /pseudo
     CDS             complement(1751..2853)
                     /locus_tag="ACHKUB_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
     gene            2882..3157
                     /locus_tag="ACHKUB_004737"
                     /pseudo
     CDS             2882..3157
                     /locus_tag="ACHKUB_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3930..4907
                     /locus_tag="ACHKUB_004738"
     CDS             3930..4907
                     /locus_tag="ACHKUB_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO6657644.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5193..5933)
                     /locus_tag="ACHKUB_004739"
     CDS             complement(5193..5933)
                     /locus_tag="ACHKUB_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6657645.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGLRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            6152..>6416
                     /locus_tag="ACHKUB_004740"
     CDS             6152..>6416
                     /locus_tag="ACHKUB_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6657646.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAE"
BASE COUNT         1565 a         1623 c         1577 g         1651 t
ORIGIN      
        1 atatagaatt tgatttttac ctgtcagaac attatcagat ggagcgcgca ggatggatga
       61 agaaagggtg ttttcactca gttatgaaca actgacccgg tttgcggaaa aacgcatccg
      121 ggaatgcaat ctggacagcc agggcgcgat ttatctctgc gagtcagcca aagctggagc
      181 cgtgctgatc ttctggcatg aactggccat aaatggctat gcctcaatga atgccataaa
      241 gagacaggag ctcattgacg ccgactttca gcgcctcaga aacctcatct ggccggagga
      301 tgactggtaa tgacccagag ccgccgaccg tcgccactgc agcggagagt gctgattgtg
      361 ctggctgccc ttgatgaaaa acggccaggc cccgtgctga cgcgggacat agaacgggtg
      421 ctggagcgaa gcggggaagc cccggtctac gggccaaacc tgcgcgcctc ctgccgtcgt
      481 ctggaggatg ccggctggct gcgtaccctg cgcgctccga acctgcagct ggcagtggag
      541 ctgacggacg ccggccgggc cgtggcacag cctctgctgc tggcagaaca ggaccgcctc
      601 cgggcagaac agcgtgcggc agaagtcgtc gtactgccgc tgatcccggc agcaggtctg
      661 cccgcagacg gcacttctgc cactgatctg gcggtgcagc tgaacggcat aacgtatcag
      721 gcctgccgtg gggattttgt ggtacgtctc gacggcagca cctgtctgca gttatggaac
      781 agagaaggca gggtggtacg ccgggagggc gatccgctgg aagtggcgca gtggctgcag
      841 gcctgtcacg atgcaggaat ggaggttcgt gtgcaggtta atgaaagcat caccccgtaa
      901 aaacatgcct gcacggttta cacatcatcc catggtggca gccggttaac tttcctgcgc
      961 caccagtgga aagaggccgt gagcagtccg aacaaaaaac cacccgtgat acttataata
     1021 atggcctcac ctgccaccat tcctgacggc ccccagtaga taaaccacat tgcacatccc
     1081 caggaaatgc cccataagcc ccccatcagc accgtgacct gccagaacgg cataaagggt
     1141 aatggcagaa gccggatacc cagccgccag agaatacgca gaagaggagg ggcataattg
     1201 ctccgccaca tctttttgct gtccatcagg gcaatggccc gggctttttt ctgctcaaag
     1261 gtcacaggag cacctccgtt ccatgatgta aacgtgacac aacttattgt tattgattat
     1321 tattatcatg ctgtggttat gttgtcatgc atcagtacat tgacaaaagt gcaaaagaaa
     1381 ggaaaacagg cagatgaaca gaaaatatta ttttaataat atgtggtggg gatggtttat
     1441 gggtgggtat actctgtata tgtcatggga ttatgagttt aaatacagat tgctgttctg
     1501 gtgtatttct ctctgcggaa tggttttgta tccggttgcc agatggtgta ttgaaaatac
     1561 tgccctgaaa tttacccggc ctgatttctg gaacagcggt ttttttgctg atactcccgg
     1621 aaaaatggga ttacttgcgg tttatacggg tactgttttc atattatctc ttccgttaac
     1681 tctgatatat attctttctg ttatcataaa aaggctgtct gtaagataga cagcctgtct
     1741 tatatattat ttaaatgccc cagaacttat tcgctttttc cacaagcgtt tcatcaatca
     1801 gtgcaccggt gacagccatc agtaaaccat acccgataat gcctaaagcg cttccggtaa
     1861 gaatactgaa gaccagagca acaagagccg ttgcggcatt acctgctatg atggtttctg
     1921 ttttaacaaa aaaggacgcc agttatctgt ccgggcagcc ttaccaaact cagtgatcca
     1981 gtcagcaaaa tttgtaattt ttcctgcata tcccagtccg cgactgaacc tgttcagatt
     2041 agacgatata tcagacagct tcacagactc aagggctgcg gcaatcgctg cacgatcttt
     2101 tgcattaatt tttttgttaa tgtcagtccg gtacttttca tacgttttta atgcctcttc
     2161 aacattacgt attttcttcc ctttagcctg cccggccatc tctctggcta actgctcagc
     2221 ttttgcacca tatttttctg aaactgattt caggaactct gttgtgaaat taattgcgtc
     2281 tttcgttgcc ttcagttcat cctgttttct tttctcttcc gctatcttct gattgatgcc
     2341 ggcaagctgg ttacgggtat tctctttttc cttcaacagg gcattaagag cgtcatttgc
     2401 atgcttttgc tcatttgttc tggcactgag gttatttctc accgaattta ccgcagattc
     2461 agcagaggtg attttatttc tggcatcaag caatctttgc cgtaacttat cccattcagt
     2521 aacttcagca ttataaattt tcttgtcacc ttccacatcc tctgtcacca ccggattccg
     2581 gtcattcaga atattgcgtt tatagtccag accggaatga gtcagaagat aattcaggtt
     2641 tgcacggtta tcgatgactg cgctggttgt gatttttatc gtatcggcaa atctcggatc
     2701 accgctcaca acaatagaat gatttgttga tgaacgcccc gggaattttt cagaggaaac
     2761 tatcgttttg ccgtcaggcg tgtttttctg ttgcgtcagc tgttgttcag ctctggttaa
     2821 tgcattacgg gccgtgttaa gcgattccgt caccgaactg agtttattcc gggcctctga
     2881 aatggccctg tcattccgtg tctgctgctc gagcagacgg gcctgttgtg catcaagaac
     2941 ggcaagggct gccccgccct ttttttccag ctctgacaac cgcgtattca gaataccttt
     3001 tctgcgctcg tattcagcaa gcatatcagc ggccttcttg tctgcgttct gcgcatcctt
     3061 ctgccgtttt tcagcctgtt caattaacga cctggcctcg cggggagata tacgaagaga
     3121 atcagcatca gcaaacagca tccggctctc tgtatgtgtc aacgccacga tgtttgaccg
     3181 tcttatatca ctggcgctga caacacgaca gagttatcag gcatggtgtc aatcagttca
     3241 caatttttcc atcggagcag cctaccaccg gtgataacct gactttgcct gtagtttgta
     3301 tacacaatcc ctggcattga aacacgcata tgaggtgtac ttcggaatat gaccgtgcgc
     3361 tcaggaagta cggcagattc cgtcaaaaat tacctgtaaa gacagacttc agaaaaaaca
     3421 gtgctcgccg cagaccggtg accgggcgaa gcccgcgaac cggcgaggaa gcgcaatata
     3481 tctgtagtta caatgagcag gtacgccctg aagccttcat aatcggtagt gtatgttgtt
     3541 tttctggaat gatttatcca catatctgga ggtccgatcc agtaaacaga tccatgaatg
     3601 atcaacaaag gatccattaa agatccccat atcgctgcaa accttgtcac tcatgggccg
     3661 ggaccacgat cacataagct gtggcatgtt actgataaac tgtaacatgc taatgataag
     3721 ctgtattcag taatccatat actgaagtaa gttaatgaca taaactatgg tcagtacgtc
     3781 agactcagtt gttaaataca ggctgcaggt ttttattcag tcagttagcg gagttctgac
     3841 acacgatttt ctgtttattc ttttactgtc cacaggctgg aggctttctg gaaaacgaaa
     3901 attcagacat caaaaaactg ttcggcgagg tggataagtc gtccggtgag ctggtgacac
     3961 tgacaccaaa caataacaac accgtacaac ctgtggcgct gatgcgtctg ggcgtctttg
     4021 taccgaccct taaatcactg aagaacagta aaaaaaatac actgtcacgt actgatgcca
     4081 cggaagagct gacacgtctt tccctggccc gtgcagaggg attcgataag gttgagatca
     4141 ccggcccccg cctggatatg gataacgatt tcaagacctg ggtggggatc attcattcct
     4201 ttgcccgcca taacgtaatt ggtgacaaag ttgaactgcc ttttgtcgag tttgcaaaac
     4261 tgtgtggtat accttcaagc cagtcatccc gcaggctgcg tgagcgcatc agtccttccc
     4321 tgaagcgcat tgccggtacc gtgatttcgt tttcccgtac cgatgagaag cacacccggg
     4381 aatatatcac ccatctggta cagtcagcct actacgatac tgagcgggat attgtgcagt
     4441 tacaggccga tccccgcctg tttgaactgt accagtttga cagaaaagtc cttctccagc
     4501 ttaaggcgat taatgctctg aagcgacggg agtccgccca ggcactctac acctttatag
     4561 agagcctgcc ccgggatccg gcaccgatat cgctggcgcg gctgcgtgca cgcctcaatc
     4621 tgaagtctcc tgtattttcc cagaaccaga cggtcagacg ggcaatggag cagttgcgcg
     4681 agattggata tcttgattac acggagatcc agcgggggcg gacaaaattc ttctgtattc
     4741 actaccggcg tccccggtta aaagcgccga atgatgagag taaggaaaat ccgttgccac
     4801 cttcacctgc ggaaaaagtc agtccggaga tggcggagaa gcttgccctg cttgagaaac
     4861 tgggcatcac gctggatgac ctggaaaaac tcttcaaatc ccgctgaata taaactgtag
     4921 tcagtgaaga gcgctccttt actgactaca gcttatatta actggtgcag tgagtggtct
     4981 gctcactgca gtttatattc ttgttccctg cagtgctgtt tgtagctgag ctgccatctg
     5041 cctgtccctt acgtaagtga tcccgtaacc tgatgctgag gcattgctcc cttcataaaa
     5101 catgacttac tcactacagc ttatatacat gctccagctt atgttatgtc tgttctgctg
     5161 accacagctt atataaggaa gcgcgtatgc cgtcacctca ggggaggcag tgtacgcagg
     5221 atctccgcag catcccgccc gtcaccggtg aaaggcactg ccagcgtggc agccatatcc
     5281 agcgcaaaca ctctggtata aacctccatc gaacgtggat ccctgtgacc gaccagtgcc
     5341 tggatgactt tccggggctg gcggtgatag agcatgtgca tgatatagct gtgccggaag
     5401 gtgtgtggtg tgaccggaat cgaaaagtga accccgtctg cttcggcccg tctgacagcc
     5461 tgcttcagcc agttgcgcat ggtttcgtcg gtcacggccc ataatggttc acgacgccgg
     5521 ggccgggtgg tgatcatcca gctttccatc tgcctgacat agcttatatc tgtcagcgga
     5581 accaggcgga cttcatcttt tggcgggcgt ccgcgtcgcg cacgcacttt ttcggacagg
     5641 attcgcacaa acggtcgcag accatccaga tcaaatgatt ccggcgtcag catccgggct
     5701 tcgccaatac gcattccggt attccagagg gtggcaaaca gcatatggtg acgctgatcc
     5761 ggcatataga agagaagggc actgacttcc ggagccagca ggtaggccgg tgtggccccc
     5821 gtggaagtgg ccattcttct cagggagagc gctgttgcaa aatccacccc cggcgcaatg
     5881 ggtaacaggg agacgcgttc tggtgaattc tgcaggggaa tgacattgtt catgagttat
     5941 ttttcacgta gcacacgcac tgtttatgga tacaggtatt ctgacctgaa cgcccggatc
     6001 cttccacgac aataaatcgc agcgcgcaga tccttttgtt tgcttatccc acattatcgg
     6061 taagcgtaca gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac
     6121 caaataagta gtgggaacca aagtatcaga tatgcagaaa aatgtgactc ccggcaggcg
     6181 aaaaggctgc cctaattatc ctcccgaatt taaacagcag ctcgttgctg cctcctgtga
     6241 acccgggata tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt
     6301 caaatggcga caacaatggc gcgagggaaa actgctatta ccttcttcag agagccccca
     6361 gctacttcct gtgactctcg atgccgctgc cgaacagcca gaatcgctcg cagagg
//
LOCUS       DAZFRN010000229         6366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFRN010000229 DAZFRN010000000
VERSION     DAZFRN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.231"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..63)
                     /locus_tag="ACHKUB_004741"
     CDS             complement(<1..63)
                     /locus_tag="ACHKUB_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657647.1"
                     /translation="MREQIKQDIDLIEILFYLKKK"
     gene            complement(277..1947)
                     /gene="betA"
                     /locus_tag="ACHKUB_004742"
     CDS             complement(277..1947)
                     /gene="betA"
                     /locus_tag="ACHKUB_004742"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO6657648.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWQEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1961..3433)
                     /gene="betB"
                     /locus_tag="ACHKUB_004743"
     CDS             complement(1961..3433)
                     /gene="betB"
                     /locus_tag="ACHKUB_004743"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO6657649.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEEEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3447..4034)
                     /gene="betI"
                     /locus_tag="ACHKUB_004744"
     CDS             complement(3447..4034)
                     /gene="betI"
                     /locus_tag="ACHKUB_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO6657650.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4163..6196
                     /gene="betT"
                     /locus_tag="ACHKUB_004745"
     CDS             4163..6196
                     /gene="betT"
                     /locus_tag="ACHKUB_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO6657651.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1315 a         1761 c         1747 g         1543 t
ORIGIN      
        1 ctttttcttc agataaaata aaatctcaat cagatcgata tcctgtttga tttgttcacg
       61 cataatatat ccagaaaata aaatctgtcg cagataaggt tgtattaata gtctgtatca
      121 ggaatgttcg ggttaaatat cagcaaaaag ccagcatcat gaatactgga tatgaagcat
      181 gagagttacc cctatgtttt atataaggat tcggtccccc tctctggaac ggtaactctc
      241 aatctgatcg gttcctgcgt tagttcacat cacgactcat tttttcgctc tcaccggcat
      301 cccatttgcc acaaaatatc ccgccgtgct cctcggcagc gcttcctgtc cacgaatcat
      361 atccgctatt ttctcgccaa tcataattgt cgtggcgttc aaattcccgg tgataatctg
      421 cggcataatc gacgcatcca ccacgcgcag gccttccagc ccgtgtacgc ggccttcgcc
      481 gtcaaccacg gacatctcgt cgtaacccat tttgcaggta ccgcacggat ggaaggcggt
      541 ttcggcgtga ttacgcacga actcatcgag ctgctcatcc gtctgacatt ccgtgccggg
      601 gctgatttcg cggccacgat actgatccag cgccggttga tgcatgatct cccgggtgat
      661 gcgaattgcg tcgcggaact cctgccagtc ctgctcgtgt gacatgtagt taaacagaat
      721 tgccggatgc tggtgcgggt cgcgggattt aatccgcaca tgcccacggc ttggcgagcg
      781 cattgagccg acgtggcact ggaaaccgtg ctccttcacc gcattagagc cgttatagtt
      841 aatcgctact ggcaggaaat ggtactgaat attcggccac gcaaattcct ctcggctgcg
      901 aataaatccg cctgcttcaa agtggttgct ggcaccaacg ccagtgccgc caaacagcca
      961 ctccgcaccg attttcggct ggttccacca ctgcagggca gggtagaggg aaaccggttc
     1021 tttgcactca tattgcaggt acatctccag gtgatcctga agattttcgc caacgccggg
     1081 taaatcatgc accagcggaa tatcaaactc cgccagcagt tcagcgctac ccacgccgga
     1141 acgttgcagg atctgcggag aggcgatcgc acctgcacat aacagcacct ctttattcgc
     1201 tgtcgcgcgg gttggaatgg tgctgtcgcc ttcctgccac tcgacgccca ccgcgcgttt
     1261 gccgtcaaaa atgatgtgat cggtcatagc gtgagtacga atggtcaggt tagggcgcga
     1321 tttggcctga tcgagataac cgcgcgcggt actggcgcga cggccctgcg gcgtgacggt
     1381 gcgatccatc ggaccaaaac cttcctgctg ataaccgttg agatcgtccg tgcgcgggta
     1441 gcccgcctgc acgcccgctt caatcatcgc ttcaaacagc ggattgacgc cgggtttgga
     1501 ggtagtgacg ctcaccggac catcgccacc gtggtagtcg ttctcgccca catcgcgagt
     1561 ctcggccttg cggtagtagg gcaggcagtc gagataactc caattctcca gaccgggttc
     1621 ttgcgcccag ttatcgagat ccagcgcatt gccacggatg tagcacatgc cgttgatcag
     1681 cgacgatcca cccagacctt taccgcgtcc gcactccatg cggcggttat tcataaacgg
     1741 ttcaggttcc gtttcatagg cccagttgta gcgtttaccc tgtagcggga atgccagggc
     1801 agcgggcatc tgggtgcgga agtcaaagcg atagtccggg ccgcccgctt caagcagcag
     1861 cacggaggta ttcggatctt cagtcagacg ggtagcgaga acgttgccgg ctgagccggc
     1921 accaataatg atgtagtcaa attgcaaata aacctcctgg ttagaatatg gactggaatt
     1981 tagccatctc aacctggatg gacttcacct gggtgtaact ctggagcgtc atcacgccgt
     2041 tctcgcggcc aatgccggag tgtttgtagc cgccaacggg catctctgcc ggggattcgc
     2101 cccaggtgtt gatccagcaa atacccgctt ccagctgatg aatgacgcga tgcgcgcggt
     2161 tcaggtccgc cgtcacgatt cccgccgcca ggccgtagtc ggtatcgtta gcgcggcgaa
     2221 tgacttcctc ttccgactcg taggtcagaa tggacatcac cggcccgaag atctcttcgc
     2281 gcacaatggt catttcgtcg ctgcaatcgg tgaacacagt cggcgcgacc catgcgccgt
     2341 tatcgaggcc atcgcctttc agtacatcgc cgccgcacag tacgcgcgcg ccttcctctt
     2401 tgcctttggc gatatagcgc agcacgttat cgcgatgcgg gaagctgacc agcgggccga
     2461 agttagtttg cggatcgaaa acgtcgcccg cgcgaatgcg ctcaacgcgc gccagaattt
     2521 tctgctcaaa tgcggctttg catttcgccg gaacgaagac gcgggtgcca ttggtacaca
     2581 cctgaccgga gctgaagaag ttcgccatca tggcgatatc ggcggcgaga tcgagatccg
     2641 catcgtcgaa aacgatcagc ggcgatttac cgcccagttc catggtcact tctttcaagg
     2701 aagaggccgc cgagttagcc atcacttttt tgccgctggc gacaccgccg gtaaatgaca
     2761 ctttggcaat gcccggatgc tcggtcagat attgcccggt ctccgcgccc acgcccggca
     2821 acacgttaaa tacgccgtcc ggcaggcccg cttcgctgta aatttcagcc agctttaacg
     2881 cggtaagcgg ggtgacttcg ctcggtttga aaatcattgc gttgcctgcc gccagcgccg
     2941 gggcggattt ccacagggca atctggatcg ggtaattcca cgcgccaatc cccgccacca
     3001 cgcccagcgg ttcgcggcgg gtataaacaa atgacgtctc acgcaacggg atctggctgc
     3061 cttccagcgc cgggatcagc ccggcgtagt actccagcac gtccgcaccg gtaacgatat
     3121 cgacggttga ggtttccgaa tatgcttttc cggtgtcgag ggtttccagt tttgcgagtt
     3181 cgtcattgcg ttcacgcaga atatcaacgg cccgacgcag aatacgcgag cgttccatag
     3241 cggtcatcgc cgcccagatt ttttgccctt gctgggcgct tttcacggcg cgatcgacat
     3301 cctcacgccc ggcggcctgc acggtcgcca gcacgttacc gttggccggg ttaatggtct
     3361 cgaaggtgcg accgctggtg gcggaggtat aaccaccatg tatataaagc tgctgttctg
     3421 ccattcggga catcacgtct cctcggttaa tcggtgggta aatgctgagt aataaagtga
     3481 cgggtcagcg agtgtgcgag aggtttatcc agcggtttgc cgctcagtgc cgcgcgcagc
     3541 cataatccgt caatcagcgc ggcaaggccg tagcccgctt cctgtgcctg ttggcgcggc
     3601 aattcgcggc gaaactcgct caccagattc gacagcaagc ggcggctgct gacctgctgt
     3661 aaacgataga gcatcggctg atgcatactg ctggcccaga acgccagcca ggctttcatc
     3721 gccgcactgc tcacctgcgt ttcatcgaag tttccgccaa caatcgcctg taatcgctgc
     3781 tctgcactgc cctgcggaag tgcatgtaat cgattcaaaa ccgcgtcacg cagctgactg
     3841 gtgatatcgc gcatggttgc ttccagcaga ccatttttgt ccctgaaata gtggctgatg
     3901 atccccgtag aaacgcctgc acggcgggcg atctgcgcga tcgttgcatc gtgcatgccc
     3961 acttcattta ttgcttccag tgtggcgtcg atcagttgtc tgcgccggat cgactgcatc
     4021 cccaatttgg gcattttcgc cactccattc atcagcggtg tttatctatt aaagcggtta
     4081 ttgattggac gttcaatata aaatgtgtct taattgttac gaatttgatt ttaaatagta
     4141 acaataacag tggggatact ggatgacaga cctttcacac agcagggaaa aggacaaaat
     4201 caatccggtg gtgttttaca cctccgccgg actgattttg ttgttttccc tgacaacgat
     4261 cctgtttcgc gacttctcgg ccctgtggat tggccgcacg ctggactggg tttctaaaac
     4321 cttcggttgg tactatctgc tggcggcaac gctctatatt gtctttgtgg tctgtatcgc
     4381 ttgttcgcgt tttggttcgg tgaagctcgg gccagaacaa tccaaaccgg aattcagcct
     4441 gctgagttgg gcggcgatgc tgtttgctgc cgggatcggt atcgacctga tgttcttctc
     4501 cgtagccgaa ccggtaacgc agtatatgca gccgccggaa ggcgcgggac agacgattga
     4561 ggccgcgcgt caggcgatgg tctggacgct gtttcactac ggcttaaccg gctggtcgat
     4621 gtatgcgctg atgggcatgg cgctcggata ctttagctat cgttataatt tgccgctcac
     4681 catccgctcg gcgctgtacc cgatcttcgg taaacggatt aacgggccga taggtcactc
     4741 agtggatatt gcagcggtga tcggcactat cttcggtatt gccactacgc tcggtatcgg
     4801 tgtggtgcag cttaactatg gcttgagcgt actgtttgat attcccgatt cgatggcggc
     4861 aaaagcggca ctgatcgcct tgtcggtgat catcgccact atctcggtga catccggtgt
     4921 cgataagggc attcgcgtgt tatcggagct taatgtcgcg ctggcgctgg gattgatcct
     4981 gttcgtgttg tttatgggcg acacttcgtt cctgcttaat gcactggtgc tgaatgttgg
     5041 cgactatgtg aatcgcttta tgggcatgac gctcaacagt tttgccttcg accgtccagt
     5101 tgagtggatg aataactgga cgctcttctt ctgggcatgg tgggtggcat ggtcgccgtt
     5161 tgtcggcttg ttcctggcgc gtatctcgcg tgggcgtacc attcgccagt tcgtgctggg
     5221 cacgttgatt attccgttta ccttcacgct gttatggctc tcggtgttcg gcaatagcgc
     5281 gctgtatgaa atcatccacg gcggagcggc atttgccgag gaagcgatgg tccatccgga
     5341 gcgcggcttc tacagcctgc tggcgcagta tccggcgttt acctttagcg cctccgtcgc
     5401 caccattact ggcctgctgt tttatgtgac ctcggcggac tccggggcgc tggtgctggg
     5461 gaatttcacc tcgcagctta aagatatcaa cagcgacgcc cccggctggc tgcgcgtctt
     5521 ctggtcggtg gcgattggcc tgctgacgct cggcatgctg atgaccaacg ggatatccgc
     5581 gctgcaaaac accacggtga ttatggggct gccgttcagc tttgtgatct tcttcgtgat
     5641 ggcggggttg tataaatctc tgaaggtaga agattaccgc cgtgaaagtg ccaaccgcga
     5701 taccgcaccg cgaccgctgg ggcttcagga tcgcctgagc tggaaaaaac gtctctcgcg
     5761 cctgatgaat tatccgggca cgcgttacac taaacagatg atggagacgg tctgttaccc
     5821 ggcaatggaa gaagtggcgc aggagctgcg gttgcgcggc gcatacgtgg agctaaaaag
     5881 cctgccgccg gaagagggac aacagttggg gcatctggat ttgttggtgc atatgggcga
     5941 agaacaaaac tttgtctatc agatttggcc gcagcaatac tcggtgccgg gctttaccta
     6001 ccgcgcacgt agcggtaaat cgacctacta ccggctggaa accttcctgt tagaaggcag
     6061 ccagggcaac gacctgatgg actacagcaa agagcaggtg atcaccgata ttcttgacca
     6121 gtacgagcgg caccttaact ttattcatct ccatcgtgaa gcgccgggcc atagcgtgat
     6181 gttcccggac gcgtgattgt tattgcataa aaccgcgcca tgtctgcata tggcgcggtt
     6241 tctcttacct cttgatacgt tatatctgta cggttaagcc cttagtatcg attgatgatt
     6301 gccaggcaac agataataaa aaagaaaaga actattgcag cccaaaacct acgtttggac
     6361 tgttgt
//
LOCUS       DAZFRN010000230         6308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFRN010000230 DAZFRN010000000
VERSION     DAZFRN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.232"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..6214
                     /locus_tag="ACHKUB_004746"
     CDS             <1..6214
                     /locus_tag="ACHKUB_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO6657652.1"
                     /translation="TGRLYSAGNLLLDAQDFSGQGQVVATGDVTLKLIAALTNHGTLA
                     AGKTLSVTSQNAITNGGVMQGDAMVLGAGEAFTNNGTLTAGKGNSVFSAQRLFLNAPG
                     SLQAGGDVSLNSRSDITISGFTGTAGSLTMNVAGTLLNSALIYAGNNLKLFTDRLHNQ
                     HGDILAGNSLWVQKDSSGTANSEIINRSGNIETTRGDITMNTAHLLNSWDAISASHEV
                     IPGSSHGVISPVPENNRWWGVVRHDGVEYLAVYWGKGATVPDEYRIRTGDTDTVTVSA
                     SGHAARISGGADMHIRAGRLDNEASFILAGGGMTLSGDTLNNQGWQEGTTGKETVWRL
                     ASGSLPKAWFTEPWYKVYRQVSPDATEASGTSPAGQYRAVISAAGDVSASFATDTGNT
                     TVMPRAGGAGNTITVPSLNSLTPPTVSQGVSGEALLNESGTGITGPVWNDALPDTLKD
                     IPGALSLSGASVSSYPLPSGNNGYFVPSTDPDSPYLITVNPKLDGLGKVDSSLFAGLY
                     DLLRMQPGQAPRETDPAYTDEKQFLGSSYILDRLGLKPEKDYRFLGDAAFDTRYVSNV
                     ILNQTGSRYINGTGSDLAQMKYLMDSAAAQQKALGLTFGVSLTAGQVAQLTRSILWWE
                     SVTINGQTVMVPKLYLSPEDITLHNGSVISGNNVQLAGGNITNSGGSINAQNDLLLDS
                     TGSIDNLNAGLINAGGALNLKAIGDIGNISSVISGKTVSLESATGNISNLTRTEQWAM
                     NNGYNHFSGTDTGPLAAVRATDSLFMGAAGDISITGAAVSAGDSVLLSAGNDLNMNAI
                     QAGERRRYGGSGWYETHAVAPTVTAGNSLMLSAGRDVNSQAAGITAENSMAIRAGRDV
                     NMAAESTGAGDHDSTFSMKTVHDSVRQQGTDMTSGGDITVTAGRDITSVATAVTAKGD
                     IRVNAGHDIVLGTATESDYHYSESGETRNRLLSHQTTRTITEDSVTREKGSLLSGNRV
                     TVNAGDNLTVEGSDVVADRDVSLAAGNHVDVLAATSTDTSWRFKETKKSGLMGTGGIG
                     FTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSITAGKQAHISGSDVIANRDISITG
                     DSVVVDPGHDRRTVDEKFEQKKSGLTVALSGTVGSAINNAVTSAQETKESSDSRLAAL
                     KGTQAVLSGVQAGVNHGLQQQSADPNNGIGVSISLNHQQSKSETKYQHDIVSGSTLSA
                     GNNVSVTATGKNKNHNNSGDILITGSQIKSGNDTSLNAQNDILLAAAADTRQTTGKNS
                     SKGGGVGVSFGGGTNGGGLSIFAGINGSEGREKGNGTTWTETTVDAGKNVSLTSGHDT
                     TLSGAQVSGEKVTADVGNNLTISSLQDSDRYDSRQNSVAAGGSFTFGSMSGSGYASIS
                     QDKIKSNYDSVREQSGIYAGKDGFDVTVGNHTQLNGAVIASTATDDKNSLSTGTLGWS
                     AIHNQADYKASHTGISLSGGSGMSASQMVASNAIAGAANALTGMSGSSGHAEGTTSSA
                     ISGGNLIIRDQESQKQDIAGLSRDPENANGSIAPIFDREKEQKRLQEAQVISQVSGQM
                     SNIVMTYGETEAMKAARKEHPGMSDAQLRETPEYREVMKGYGTGSTPQMVVQAITGVL
                     GGLNAGNPGQVLAGGLNPAVAQLIKQATGDNREANLMAHAVWGALAAQLGGNNAASGA
                     AGAFSGELAARYIIDNYYGGRTDNLSEQERQQISMLATIASGIAGGLAGNSTSAAGTG
                     AQAGRNSVENNYLSVSEKTELEIAKQKLKNSKDPAEREKAQQKYDALLEKDISSDKAV
                     ITACSNGQAASAACAGERLKVIAAKGGYETRHYNNQVSDMYPDAYGQIVNLLNITSVD
                     AQNQQQVKDAMVNYAMVQFGVDRATAQAYVETYDGMKVVAASMAPVIGAAAASKIEVL
                     AGKQRLSNSFEVSSLPDANGKNHITAVKGDAKIPVDKIELYMRGKASGDLDSLQAEYN
                     SLKDARISSQKEFAKDPNNAKRMEVLEKQIHNIERSQDMARVLEQAGIVNTASNNSMI
                     MDKLLDSAQGATSANRKTSVVVSGPNGNVRIYATWTILPDGTKRLSTVNTGTFK"
BASE COUNT         1656 a         1538 c         1848 g         1266 t
ORIGIN      
        1 tacagggcgg ctgtacagtg caggcaacct gctgcttgac gctcaggact tcagtggtca
       61 gggtcaggtg gtggccaccg gtgatgtcac actgaaactg attgctgccc tcacgaatca
      121 tggtaccctg gccgcaggga aaaccctttc cgtcacgtcg caaaatgcca tcaccaacgg
      181 cggtgtgatg cagggtgatg ccatggtgct cggtgccgga gaggcattca ccaacaatgg
      241 aacgctgact gccggtaaag gcaacagtgt tttcagcgca cagcgtcttt tccttaacgc
      301 accgggttca cttcaggccg gtggcgatgt gagtctgaac agccggagtg atatcaccat
      361 cagtggtttt accggcacgg caggcagtct gacaatgaat gtggccggta ccctgctgaa
      421 cagtgcgctg atttatgcgg ggaataacct gaagctgttt acagaccgtc tgcataacca
      481 gcatggtgat atcctggccg gcaacagtct gtgggtacag aaggacagca gcggtacggc
      541 aaacagcgaa atcattaacc gttccggtaa tattgaaacc acacgtggcg atatcaccat
      601 gaatacggcg catctgctga attcctggga tgccatttcg gccagccatg aggtcattcc
      661 tggaagcagt cacggggtga tttcaccggt tccggaaaat aaccggtggt ggggtgttgt
      721 ccggcatgat ggtgtggaat atctggccgt gtactggggt aagggtgcca cggtacctga
      781 tgaataccgt atccgtacag gcgatacgga tacggtgaca gtcagtgcct ccggtcatgc
      841 agcccggata tccggcggcg ctgatatgca tatccgtgca ggacgactgg ataatgaggc
      901 atcgtttatt ctcgccggtg gcggtatgac cctgagtggc gacacactga acaatcaggg
      961 ctggcaggag gggaccaccg gaaaggaaac ggtctggcgg cttgcctcag gctcactccc
     1021 caaagcgtgg tttactgagc catggtataa ggtctatcgc caggtctcgc cggatgcgac
     1081 agaagccagt ggcacctctc cggcaggtca gtaccgtgcg gttatcagcg ctgccggtga
     1141 tgtgtctgcc agctttgcca ctgacaccgg taataccact gtcatgcccc gggcaggtgg
     1201 tgcaggtaac acgataacgg tgccttcgct gaactccctg acaccaccga cggtcagtca
     1261 gggggtgagc ggtgaagccc tgctgaatga aagcggtacc gggataaccg gccctgtatg
     1321 gaatgacgcg ctgccggata cgctgaagga tatcccgggt gcgctgtcgc tgtccggggc
     1381 aagtgtaagc agctatcccc tgccatccgg taacaatggt tattttgtcc cgtccacgga
     1441 cccggacagt ccgtatctga ttacggtgaa cccgaaactg gatggtctcg gaaaggtgga
     1501 cagcagtctg tttgccggac tgtatgacct gctcaggatg caacccggac aggcaccacg
     1561 agaaacagac ccggcatata ccgatgaaaa acagtttctg ggctcgtcgt acattcttga
     1621 ccgtcttggc ctgaaaccgg aaaaagatta ccgctttctg ggggatgcgg cttttgatac
     1681 ccggtatgtc agtaacgtca tactgaacca gacgggctca cgttatatca acggcacagg
     1741 ctctgacctg gcgcagatga aatacctgat ggacagcgcg gcagcacaac agaaagcgct
     1801 cggactgacc tttggtgtgt cgctgaccgc cgggcaggtt gcacaactga ccagaagcat
     1861 actgtggtgg gagtccgtca ccatcaacgg acagacagtc atggttccga aactgtatct
     1921 gtctccggaa gatatcaccc ttcataatgg cagcgttatc agcgggaaca acgtgcagct
     1981 tgcgggtggc aatattacca acagcggcgg cagcatcaac gcacagaatg acctcctgct
     2041 cgacagcaca ggcagtatcg acaacctgaa tgccggactg ataaacgccg gtggtgcact
     2101 gaacctgaag gccatcgggg atatcggcaa tatcagctca gtcatcagcg gtaaaaccgt
     2161 atccctggaa agcgccaccg gaaacatcag taacctcacc cggacagaac agtgggcgat
     2221 gaacaacgga tataaccatt tcagcggaac ggataccggt ccgctggctg ctgtcagggc
     2281 aacggactca ctgtttatgg gcgcagccgg ggatatcagt atcaccggtg ccgctgtttc
     2341 tgccggtgac agcgtgttac tgtccgccgg taatgacctg aacatgaacg cgatacaggc
     2401 cggcgaacgt cgtcgctatg gcgggtccgg gtggtatgaa actcatgccg tggcccccac
     2461 ggtcactgcc gggaacagtc tgatgctttc cgccggtcgt gatgtgaaca gccaggctgc
     2521 cggtattacg gctgaaaaca gtatggctat ccgcgccgga cgggatgtga acatggctgc
     2581 ggagtccaca ggcgcaggtg accacgacag cactttcagc atgaagacgg ttcatgattc
     2641 ggtccggcag cagggaacag acatgaccag tggcggtgac attacggtta ctgccggacg
     2701 ggatatcacc tcagttgcga ccgctgtaac ggcaaagggc gatatccgtg tgaatgccgg
     2761 tcatgacatc gtactgggta ctgccacgga aagtgattat cactacagtg aatcagggga
     2821 aacccgtaac cgtctcctca gccatcagac cacccggacc atcacggaag acagcgtcac
     2881 ccgtgagaag ggctccctgc tgtcggggaa ccgcgtgacc gttaacgccg gtgataacct
     2941 gacggtagag ggttcggatg tggtggctga ccgggatgtg tcactggcgg cgggtaacca
     3001 tgtcgatgtt cttgctgcca ccagtacaga tacgtcctgg cgctttaagg aaacgaagaa
     3061 atccggtctg atgggtaccg gcggtattgg ttttaccatt ggcagcagta agacaacgca
     3121 cgaccgccgc gaggccggga caacgcagag tcagagtgcc agtaccatcg gctccactgc
     3181 cggtaatgtc agtattaccg cgggcaaaca ggctcatatc agcggttcgg atgtgattgc
     3241 gaaccgggat atcagcatta ccggtgacag tgtggtggtt gacccggggc atgaccgtcg
     3301 tactgtggac gaaaaatttg agcagaagaa aagcgggctg acggttgccc tttccggcac
     3361 ggtgggcagt gccatcaata atgcggttac cagtgcacag gagacgaagg agagcagtga
     3421 cagccgtctg gcggcactga aaggaacaca ggcggttctg tccggtgtac aggccggtgt
     3481 taaccacgga ctgcagcaac agagtgctga cccgaataat ggtattggcg tgagcatttc
     3541 gctcaaccat cagcagtcga agtctgagac gaaatatcag cacgatatcg tctccggctc
     3601 aaccctcagc gccggtaaca atgtctccgt cactgccacg gggaaaaata agaaccataa
     3661 caacagcggc gatattctca ttaccgggag ccagattaag tccggcaatg acaccagcct
     3721 gaatgcacag aacgatattc tgctggctgc cgccgctgac acccgacaga ccaccgggaa
     3781 gaacagcagc aaaggtggcg gcgtgggcgt cagctttggt ggtggaacca atggcggagg
     3841 cctcagtatc ttcgccggta tcaatggttc agagggcagg gagaaaggca acggtaccac
     3901 ctggaccgaa accacggtgg atgcgggtaa aaatgtctcc ctcacaagcg gacatgatac
     3961 caccctgtcg ggggctcagg tcagtggcga gaaagtcact gcagatgtcg gcaacaacct
     4021 gaccatttcg agccttcagg acagtgaccg ttacgacagc agacagaaca gcgtggccgc
     4081 aggtggaagc ttcacgtttg gctcaatgtc cggcagcggc tatgccagca tcagtcagga
     4141 caaaattaaa agcaattatg attctgtccg ggaacagagt ggtatctacg ccggaaaaga
     4201 cgggtttgat gtcactgtgg ggaatcacac tcagctcaat ggtgcggtaa ttgcgtccac
     4261 ggcaacggat gacaaaaaca gcctcagtac cggcacgctg ggctggtcgg ctatccataa
     4321 ccaggcagac tataaagcca gccataccgg aatcagttta tcgggtggct cagggatgtc
     4381 agccagccag atggtggcct caaatgcaat agccggtgcg gcgaacgcac ttaccggtat
     4441 gtccggcagc agtggacatg ctgaaggtac cacgtcatcc gccatcagcg gcggaaatct
     4501 cattatccgt gatcaggaga gtcagaagca ggatattgcc gggctcagcc gggacccgga
     4561 aaatgccaac ggcagcattg caccgatatt tgacagggag aaagagcaga agcgtctgca
     4621 ggaagcgcag gttatcagcc aggttagcgg tcagatgagt aacatcgtca tgacgtatgg
     4681 cgaaactgaa gcgatgaaag cagccaggaa agagcatcct ggcatgagcg atgcacagtt
     4741 gcgggagact ccggaataca gagaggtcat gaaaggctac ggtacgggca gtacgccgca
     4801 gatggtggta caggccatca cgggcgtgct gggcggactg aatgcaggga atccggggca
     4861 ggtactggct ggcggtctga atccggcagt ggcacagctt ataaaacagg ccaccggcga
     4921 taaccgggaa gcaaacctga tggctcacgc agtctgggga gcacttgccg cacaactggg
     4981 cggaaacaat gcggcttccg gtgctgcggg ggcattcagc ggtgaacttg cagcccggta
     5041 tatcattgat aactactacg gtggtcgtac cgataacctg agtgaacagg aacgtcagca
     5101 aatcagcatg ctggccacca ttgcttcagg tattgcgggg ggacttgcag gcaacagtac
     5161 aagtgctgcc ggtacggggg cacaggcagg caggaactcg gttgagaata attatcttag
     5221 cgtgtctgaa aagacagagc ttgagatagc aaaacagaag cttaaaaaca gtaaagatcc
     5281 ggcagagcgt gaaaaggcac agcagaaata tgatgcattg cttgaaaaag atatcagcag
     5341 tgataaggca gtaatcactg cgtgcagtaa cggacaggca gccagcgcag cttgtgccgg
     5401 tgaaagactg aaggttattg cagctaaagg aggttatgaa accagacact acaataacca
     5461 agtcagcgat atgtatccgg atgcttacgg gcagatagta aacctgctga atatcaccag
     5521 tgttgatgca caaaatcaac aacaggtgaa ggatgcgatg gtgaactatg ccatggtgca
     5581 gtttggggtc gatagggcga cggcacaggc atacgttgaa acttatgatg gaatgaaagt
     5641 tgtagctgct tcgatggcac cagtaatagg tgctgcggcg gccagtaaga tagaagttct
     5701 ggctggtaaa caacgcttgt ctaatagttt cgaggttagc tcacttccag atgcaaatgg
     5761 gaaaaatcac atcacggctg taaaaggtga tgctaaaata cctgtcgata agattgaact
     5821 atatatgcgt ggtaaagctt ctggtgacct tgattctttg caagcagaat ataattcttt
     5881 aaaagatgca aggattagca gccaaaaaga atttgcaaaa gatccaaaca atgctaaaag
     5941 gatggaagta ttagaaaaac aaatccataa tattgagcgt tctcaagata tggctcgtgt
     6001 attagaacaa gcaggtatag tgaatactgc cagtaataac agcatgataa tggataagtt
     6061 attggactca gctcaggggg ctacatctgc aaatagaaaa acatctgttg ttgtatcagg
     6121 gcctaacggt aacgtaagga tttatgctac atggacaata ttacctgatg gcacgaaaag
     6181 actttcaact gttaatacgg ggacattcaa atgataaatg tgaatagtac cgcaaaggat
     6241 attgaaggtt tagaaagtta cctggctaat ggctatgtag aggctaactc ttttaacgac
     6301 ccagagga
//
LOCUS       DAZFRN010000231         6231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFRN010000231 DAZFRN010000000
VERSION     DAZFRN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.233"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(22..390)
                     /locus_tag="ACHKUB_004747"
     CDS             complement(22..390)
                     /locus_tag="ACHKUB_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6657653.1"
                     /translation="MSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYVKRLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            complement(470..691)
                     /locus_tag="ACHKUB_004748"
     CDS             complement(470..691)
                     /locus_tag="ACHKUB_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6657654.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(778..1254)
                     /locus_tag="ACHKUB_004749"
     CDS             complement(778..1254)
                     /locus_tag="ACHKUB_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6657655.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIIGGSQVFSFAEHGLL"
     gene            complement(1269..1748)
                     /locus_tag="ACHKUB_004750"
     CDS             complement(1269..1748)
                     /locus_tag="ACHKUB_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6657656.1"
                     /translation="MKIVSQNTTALSSATALGNNQPQLVATPVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDNDETWVLFNVMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(2014..2832)
                     /locus_tag="ACHKUB_004751"
     CDS             complement(2014..2832)
                     /locus_tag="ACHKUB_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6657657.1"
                     /translation="MRLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYCKDLWS
                     TYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(2922..3155)
                     /locus_tag="ACHKUB_004752"
     CDS             complement(2922..3155)
                     /locus_tag="ACHKUB_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6657658.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDDQGTNFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            complement(3161..3838)
                     /locus_tag="ACHKUB_004753"
     CDS             complement(3161..3838)
                     /locus_tag="ACHKUB_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657659.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNSWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGTDGNATGA
                     SVEFIGK"
     gene            complement(3986..4666)
                     /locus_tag="ACHKUB_004754"
     CDS             complement(3986..4666)
                     /locus_tag="ACHKUB_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6657660.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
     gene            complement(4869..5753)
                     /locus_tag="ACHKUB_004755"
     CDS             complement(4869..5753)
                     /locus_tag="ACHKUB_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6657661.1"
                     /translation="MPFSNSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRHQELSLATVTVRVATLFPSSFPVLPVAAPAGWNLPALVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDDAVARFSFPAWMLQLLR
                     KARDRIIHLLITLWERLF"
     gene            5854..>6231
                     /locus_tag="ACHKUB_004756"
     CDS             5854..>6231
                     /locus_tag="ACHKUB_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657662.1"
                     /translation="MAKNKGICRNKKCSNPVSEFDEANALRLCDDHYKLWKRNKENRR
                     VRNICRIISKYDKSLDREIFYGVRDAAKNKTGKRIIEDAKILHQCRASKQVRKSFHKG
                     VLRSREWLNTQELLSRKHISVTGE"
BASE COUNT         1537 a         1501 c         1698 g         1495 t
ORIGIN      
        1 gcagaaggtt attctggaga gttacttttt cgtttcgggc gtcggataaa cagccatata
       61 aacgtagcca caactgccga gggtgtcggc ttcgcaggtc agccttttta catacagcgt
      121 gacggtatgc tgatggcggg gattcagttc accgctggtg agcatgagtt ccagttgttt
      181 catcagcagc ggaaaggcct ggtccaggtg gtacgaatcc gcgtcgctga accgccctct
      241 gataccggcg cggtcggcaa ggtaatgcaa ccggttgcct tcctgtacca gacgtgcccc
      301 aaaacagggc gtcacggtgc agggcagtcc ccaccaggtg cggtcgttat tgtcgtctgg
      361 aagcgttgtc ccggggagtg tgtctgacac gataaaatcc tcacagaaaa ttggttaaga
      421 atggtccggt ggtggcgata attctgctct tctgaatgcc cttccagact cagttcaggg
      481 tgacgctcat cagccggaca tacggaccaa aactgtcctt ccggcgttct gcaaacacag
      541 ccagcacacc gggaatatcc tgtacttcac ggccggtata tgtatccgtg ctaccgtgcc
      601 agcggtattt accggtacag aacggaaaaa gacgggatgc aggatgctga cggtgaatac
      661 gcatggcttc accacgggtg atgattttca tgagaatagt cctcctgaat gtgaaagtaa
      721 gaaacagctg agaaagagaa actgaagtga tatgaaggtg attgtgacgg tgacgggtta
      781 aagcagaccg tgttccgcaa aggagaaaac ctggctgcca ccgattatca gatggtccgg
      841 tacccggata tccaccaggg ccagtgcctg taccagacgc tcagtgataa ggcggtctgc
      901 cttgctgggg gtgacttcac cggacgggtg attgtgcgcc agcaccacgg cagcggcatt
      961 gtggtacagg gcgcgtttaa tcacctcccg gggatggact tctgtgcggt tgatggtgcc
     1021 agtgaagagg gtttcaccgg caatcagctg attctggttg ttcagataca gcacccggaa
     1081 ctcttcccgc tccagtcccg ccatgttcag tatcagccat tcccgtgccg catgcgtgga
     1141 ggtgaaggcc acgccgggtt catgaagatg gcggtccagg gttttcaggg cccgctgaat
     1201 gaggctgcgc tcaccgggcg tcatttctcc gggcagaaag gaaatctgtt gcattgttcc
     1261 ggcccctttc agtcaatgat gcgcataatg gcgctgcatt ctggatgctg cagggcgtaa
     1321 tcccgcagcc ggtaataatg gaccgtcatg gcataacatt ccgtacgaca ggcatgatgg
     1381 ctgtacgtca tcagacaggc ggcaataccg gcggcttccg ggctcatttc agcgcggtta
     1441 ccgttcatga cattgaacag tacccatgtt tcgtcattat cgtcatccgg ttcaggtgcc
     1501 ataaatgcgc caccgttgtt cagggtgtac agattccaga tgcccccgca gtagtcttcg
     1561 cacagacggt ccatccagcc gaagacacgg ggctccaggg tcacccactg tggaatgagg
     1621 ccaaaatgct gcggccagaa gctgatacgt tgttcatcag ggacgggagt agcaaccagt
     1681 tggggctggt tattccctag tgctgtggct gaagaaagag cagttgtgtt ctgagaaacg
     1741 attttcatga gtgaaatcct tacaatgtgt ataaatgaga ggaaataaag gccgggggct
     1801 gacaacatca cgttcatcac tgaccgtgag acccggcaat atcagggatg aatgatattc
     1861 ggctgaaatt ccggacagcc gtacgcgtaa ccgttccggt cacagacgga tggccagaag
     1921 atgccattgc ctgtactcag tctgcacaga ctgagttctc cggggcgtcg ggtgattctg
     1981 gtgcctgaat cacgccttca gggacagatt gcatcagcgc aggctctcca gtagcgtttc
     2041 tgccatcacc cacaatgcgc gattgagctt aatgtcggtg tcgatgctgt gaatggcacg
     2101 ggtgtggata cgttttcctc tggcactgcg accggaaatt ccgcctttca gcatattctc
     2161 ctggatggtc tgataagtac tccacaggtc cttacagtaa tcctcccggc gtcgtggcgt
     2221 cagaatgtcg gcggtggtga cgggctgatg ttcgtcacca taacggtaag tcagtgccgc
     2281 ctgtgccagc gcctggcgtg ccggtggcgg caggaccagc gactgcatgg catcacgctt
     2341 ctcctctatc cggtcaaaca cgcccaccac ctcgtaagcc ccttcaataa ctttctccac
     2401 cacatttccc cggtgtggaa cacgcaattc tcccagagac tgaccgcaga ccagtccatt
     2461 ggtacaaatg gcacgaaaat atccgggtaa catctggtag ctggatgaac cgtcatggga
     2521 gttgagcaga ataatttcag gcacatgctg accggttatc tgtccggccc gccgcagacg
     2581 cagcatatgt ttggtatatt cccggcggct ctggtcgcgc acacgggtct ggcaggcgaa
     2641 gaacggctga aagccttccc gctgcagatt ttccaggacg gtgatggtgg gaatgtacgc
     2701 ataccgttca ctgcgggagg tgtgccggtc ttctccaaaa atactcggga cgtggtacat
     2761 cagttcttcg cgtgtcagtg ggcgctcccg acggatctga tttacatgac caaaacgact
     2821 ggctaatcgc atactttgct ccttatgaat gaattagcgg tataaacgaa aaggccgcgt
     2881 ctcccggaga agacgcggcc tgatggatga aatgaagacg tttattgcct gagaattccc
     2941 tcactggcga gatacgaatt tagctgtttt ccggcatcag gttcaaaatt ccagcaccgc
     3001 cagcgtagct gcccttcggc attgcggata accagccgga aattcgttcc ctggtcatct
     3061 tcaataagca cattgcggta agctgttgtg acagccatcg cctgttcgcg tgagaaaggg
     3121 ccttcgggca ataaaacagg gtgacagtcc gacataccgg ctattttccg ataaattcaa
     3181 cgctggcgcc ggtggcatta ccgtcagtac caaccgtgac ggtcactttg tcgtacgcac
     3241 ggtcttcagg attgaacact tcaaactcaa ccttattgcc ttttacttcg taagagccaa
     3301 aatcagctgt ttctgttgtt tcctcgtcat attcccgggc gtatgcctgc cgggcagcct
     3361 gcaggacgga gtctttatta atagtggtcg tctgtttcgg ggaaaaaccg gcaagtgatg
     3421 tgacaaattt cggacagacc ttcagcatat caccgcgggt gaattccatt cgtacaccaa
     3481 atgcctgtaa aacggacaca tcacgctctg ctatggagtg aacggctttt ttgaccattt
     3541 ccggcgcctg tccgatttcc acctcaacag ccctgaatcc cgtctgctga ttacacattt
     3601 gtgatgtggg gatcaggtta ccgttaatac tgatacggct gcccaggagc gtctgtacgc
     3661 cattcgggca tccctgcatc aggccggtaa taccaatcgt gccatttttc agcattacca
     3721 gcgcctgacc atcttccgtc atggtctgac accagctgtt tttgacagcc cagttgccgg
     3781 cggaggcgga gaatgatgcc acagtgaaca gcaggggaaa aagagctctg gtattcatga
     3841 ctgacctctg aaatgaacgt acggtgttat tgataatgat ggaaggtatg cagtgactgt
     3901 tcagggaatg acgtagccgt atatttagtt ttctgttcaa catattgtct ccatacctgt
     3961 cgggacggct ttggcaggcc ggcgatcaca ggttaaaggt gttgataagt ttatggatga
     4021 aagagaaatg tggcagggcg gagataaaac gctcttcagc gaccagactg tggatgtggc
     4081 ctctccgttc aaaccgttct gatgtcaggc tgactgattt gatatctgcc agaggaaaga
     4141 cctgcagttt tcccttctgt aacgcgatca gataccagct accgctgtaa taaatgagcc
     4201 ggcagggctc cagtgacgga taccatcgct cagtcatcag agagatgtgg atacactgtc
     4261 ttattgccag gatgagttgc gagaaacact cggggaacgt tgcagtgata tccggcgccg
     4321 gtagccagat aaggcaggga gagggctcct ggttgtcggt aagacaggtt atcagtcgtc
     4381 cgttccggag cggaagtatc cgtgcgatcc cggtattctg tataaaagaa agcatttcag
     4441 ggatcgcacc cgggctgctc tgtcgtctga ggctgtacct gccatttctg tactctaaat
     4501 ccaggtgaac cagacgctga tgaaaatcgc gctgtaaagt acgttctgta acgccaaatt
     4561 catctgacag tgtttttagt gacagagatt ctccggccat cagtcggctg ataatgagtg
     4621 ataagcgaac cgccaggcga tcatgccggg aacgattatc agacatagat gtacactccg
     4681 taagaaaaca tcttttattt catgatgtta ttatgcatca tgatgttgaa aggatgtgtc
     4741 gcatccggat aatcaggaat aaaatgcagc agaaaaaata ttcttttaat tatctgcttt
     4801 ttaagcatga tttcataaaa agaagaatgc tcgttaatcc agcgacacat cctgtcggcg
     4861 tgagtgtgtc agaacagacg ctcccacagt gtgatcagca ggtggataat gcggtcccgg
     4921 gctttacgca gaagctgtaa catccaggcc ggaaaactga accgggcaac ggcgtcgtca
     4981 aaacttttcc cgatggcatc accaaaagtc tgttgtgcgt gtttccgggc ctgttcagag
     5041 cggttttccc ccctgatatg tgaataaact gcgctggttg cctgtggtgg cagcgcgtgg
     5101 atcatcagtg acaccagcgc tggaaggttc cagcctgcag gcgcggctac agggagtacc
     5161 ggaaatgatg aagggaacag ggtggccacc cggactgtaa cagttgccag tgagagttcc
     5221 tggtgacggg acgggcattt ttctgtggca ttccattctt cagcaggaaa cacgcgatcg
     5281 gcatggctga gaacaaacag aaaacgcgag ggatctgcac cttcattcag taaaaactga
     5341 tgcatggcaa tatcggcagc atacgcacgt tcatcagccc gcaggatcca gataatcaga
     5401 tccagttccg gcagtaactg gcggtaaagc tcccggtatt cctgatcatg ctgcggtgtt
     5461 tcaccaatgc cgggcagatc gaccagcgtc attctgcgtt caccaatctg cagggtgagg
     5521 cgatgagcct ggcgggtgca gccgttcagg ggatgcgtgg cgcagatacg ggactgaaaa
     5581 atggcattac acagactgct tttgccggtg ccggatttac ccataatgcc aatcacgggc
     5641 tcgtaatgaa taacaccgtg gagatgttca aggattttct ggcgtatttc ttcaggaaga
     5701 aacgtcagat atgatttcac ttgcgcggag agggaagaat tgctgaatgg cataccggta
     5761 tcctgttgat ttcactgaac cggatcacac taatccggtt tatcattcag gtaatatggg
     5821 tgtgaaaatt tttccattga cgggaagaag agtatggcta aaaacaaagg tatttgtcgg
     5881 aataaaaaat gcagtaatcc tgtttcagaa tttgatgagg caaatgcctt aaggttatgc
     5941 gatgatcatt ataagctatg gaagcgaaat aaagagaatc gtcgggtcag gaatatttgt
     6001 cgtataatca gtaaatatga caaaagtctg gaccgggaga tattttatgg agtaagagat
     6061 gctgcaaaaa ataaaacggg aaagagaatt attgaggacg caaaaatatt acatcaatgc
     6121 cgtgcctcaa agcaagtaag aaaaagtttt cacaagggtg tgcttcggtc acgggaatgg
     6181 cttaatacac aggagttact gagcagaaaa catatatctg ttacaggtga a
//
LOCUS       DAZFRN010000232         6205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFRN010000232 DAZFRN010000000
VERSION     DAZFRN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.234"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            199..414
                     /locus_tag="ACHKUB_004757"
     CDS             199..414
                     /locus_tag="ACHKUB_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6657663.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            588..845
                     /locus_tag="ACHKUB_004758"
     CDS             588..845
                     /locus_tag="ACHKUB_004758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657664.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            827..1018
                     /locus_tag="ACHKUB_004759"
     CDS             827..1018
                     /locus_tag="ACHKUB_004759"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6657665.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1160..2179
                     /locus_tag="ACHKUB_004760"
     CDS             1160..2179
                     /locus_tag="ACHKUB_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO6657666.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2900..3115
                     /locus_tag="ACHKUB_004761"
                     /pseudo
     CDS             2900..3115
                     /locus_tag="ACHKUB_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3290..5260
                     /locus_tag="ACHKUB_004762"
     CDS             3290..5260
                     /locus_tag="ACHKUB_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFO6657667.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5279..6070
                     /locus_tag="ACHKUB_004763"
     CDS             5279..6070
                     /locus_tag="ACHKUB_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO6657668.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1842 a         1375 c         1499 g         1489 t
ORIGIN      
        1 gtctgtcttc tttttaatat taacttatta attggtgcta ttcatataat tctttaaaca
       61 gcgaacttat agccaggatt caatagatat agtaacagga acatcagtaa gcgatgagat
      121 ggtcttcccc ctccttcacg acaaccttta attacgtcat gatggataaa ctttaaccac
      181 aaaagtaaga gggataccat gtctcaacag aaaatcaccg gcatcgatct ggctaaaacg
      241 aacttttatc ttttcagtat caatgctcat ggcaaacccg caggaaggat caaactttct
      301 cgcaatcaac tgcttaactg gctggtgcag cagcctaaaa tgactgttgc gatggaggct
      361 cgcgggcatc ccatcactgg gccagggaaa tcaggaaact cgaccatgat gtaatatttc
      421 ttccggcgca acatgttaaa gcttatcagc gacgccagaa aaatgattat gacgacgctc
      481 aggcaatagc tgaggcctgc caacatggaa ctatacgccc cgtaccaata aaaactctgg
      541 agtaacagga cgtccagact tttttgaaaa tgcgccggtt ggtttcgatg gaaaggacac
      601 aactgataaa tcacgttcgt ggacttcttg ctgaatatgg cattgtgttc agtaaaggcg
      661 caacagaatt acgccaaaaa ttaccggcat tacttgagga tgcggaaaac gaactcactg
      721 ataccatgaa gacgttattg catcggcagt atatacggtt gattacacta gataatgaac
      781 tggagtggta tgattctgaa ctgaaaaata tgtccgccaa gatcctatgt gcaaacgctt
      841 gttagccatt cctagattca gccctctggt tagtcaggca ataaaaagct ggatggggga
      901 tggaaaacag tttagacgag gaagagatgc atctgcgact ctgggactgg ttccccgaca
      961 gttcagcaca ggtgggaagc aggttcttct gggtataaca aaatttggag aaatgtaatg
     1021 aacacatccc accccattaa gtcaggtgct ctgtgatgga tgaagggaaa tactggaagg
     1081 tgtggagcct cgcagatttc cggcatcata gtgcccaata caggatgcat aaagctccct
     1141 cagactggag actccacaaa tgaaatatac accggttggc gttgatatcg caaaacatct
     1201 gattcaggtt catttcatcg acgagcacac aggtgaagtg attgataagc aactacgtcg
     1261 tcaggatttt ctgatgttct tctgtaatcg ggaaccctgc ctgattggta tggaggcctg
     1321 tagaggctct cagcactggg ggcgtgaact gacaaagctg gggcataaag tccggttgtt
     1381 acaggcccac tttgttaagg cattcgtcat gggaaataag aatgatgtga tggatgcccg
     1441 ggctatctgg atggctgttc agcagccagg taaggacatc gcggttaaaa cagaagaaca
     1501 acagtcagtg ctggttttgc accgttcccg cagacaattg gttaagttcc gcaccatgca
     1561 gattaattct ctgcacggca cgctcctaga atttggtaaa accattcaca aaggtcgggc
     1621 agcgatggag cgagagctcc ctgaagcact ggagcggatg aaagagagac tgccgccgta
     1681 tctcattact gtgctggaaa accagtacaa ccggctgaat gagctggact cgctgataga
     1741 ggatattgaa aaaaagctta ccagcgtggc gagacagaat gaaacctgta agcggttgct
     1801 ggatattccc ggcgttgggc cacttattgc aacggcagcg gtggccacca tgagggaagc
     1861 atcagcgttt aaatcagggc gagagttcgc ggcttatgtt ggtctggtgc ccaaacagac
     1921 tggctcagga gggaaaatcc gcctgctgga gataagcaaa cgtggtaata catatctgcg
     1981 agccttgttt attcatagcg caagggcggc agcattactg cccaaagaac ctggtccgtg
     2041 gataaccgaa ctgaaaaaac gtcgtccaac cagcgtggca atcgtcgcca tggcaaacaa
     2101 actagcacga acagtatggg cgatagcagc tcatggaagt aaatacgaca agaatcatgt
     2161 cagtatcaga tcatattaat cgctgatacc gataaatatt aacttttaat gaaaggatga
     2221 atactgaaag gtaactctgg cggccatagt gatgacaacg acaggtaaga ccgggactca
     2281 ctaaacctga atattgtgtt gagctaaaag ctcgctggag aaatgaggtg tgagtcagcg
     2341 aattacatag gggctcgcag cttcatggct gcaaaaaagc cggatataaa gctgcaacct
     2401 acccgtcact gtcaaggcaa tggatgcctt gcaacgggat gtgttcatat aatcaatatg
     2461 tcagatctat gctgatacag ggtgccagag cggtattgtt cagagcctca gagaaaactg
     2521 attcacttcg tgtgtgggtt aattgtattc gggaaaagcg tggcttcaac caggcagttg
     2581 ttgctcttgc aaataaactc cttcggatcg cctgggcaat tatcgcccgc aatgagatgt
     2641 atttgcccgc agcaaaacaa aatcaataat ctacgtaaaa tcagtaggag taaagcttac
     2701 cctgaaattg caaagaatgc agaatgatga aaaacaggtc agaccgacgt tacgaaaacc
     2761 tgcaagatga gccaggccag taaggccgag taagcgataa ggtcggagcg cgcgaatgtc
     2821 tcatcaaggc cagaagcaat aacttcatga acaaaccgga tatacgaaag taaacctgtc
     2881 cctatcatag ttgctgtact cttgcaatac gggggaagac catctcatcg cttacgaaca
     2941 attcgtgaaa acgatgaaga aagactacat cgcgtgcatg ccgaaatcta atgtaagaac
     3001 ggcattgcat aatcttgcag tggagatcgg gtattacaat gaaaaccatc cgcatagtgc
     3061 gttaggttat ctcccaccaa gggaatatca atgtcagcga ataacgtcac cttagatgca
     3121 gtagttgtca ggaaatagtg actcaagggc aggcttacca tatacactct tagtggttcg
     3181 actgggtatt agaatagcta ttaacagatt agcactgaac actgcgtaat agtggttgca
     3241 caaacaaaat gttatgttat aacattgctg tattgacata tattttcgaa tgtatatgaa
     3301 cgtaatcaga actgtcattt gtacattaat catacttccg gtggggttac aggcagcgac
     3361 aagtcattct tctatggcta aagatacaat caccgttgtc gcgacaggaa atcagaacac
     3421 ggtatttgaa acgccgtcga tggtcagtgt catcacgaat gacacgccat ggagtcagaa
     3481 tgcggtcaca tcagccggta tgctgaaagg tgttgcgggt ctcagccaga ctggtgcagg
     3541 acggaccaat gggcagacct ttaatctacg cggctatgac aaaagcgggg tacttgttct
     3601 tgttgacggc gtccgtcaac tcagtgacat ggcaaaaagc agtggcactt atctggatcc
     3661 ggcactcatc aaacgcatcg aagttgtccg cggaccaaac tccagtctgt acggcagtgg
     3721 cgggctgggg ggggtggtgg acttcagaac tgccgatgct gcagattttc ttccccccgg
     3781 agagacaaat ggtgtaagtc tgtggggaaa tatcgccagt ggtgaccaca gcacaggctc
     3841 ggggctcacc tggttcggta aaaccggaaa aacagatgcg cttctttctg tcattatgcg
     3901 taaaagagga aatatctatc agagtgatgg tgagcgtgca cctaacaagg aaaaacctgc
     3961 agccctgttt gcgaaaggct ctgtcagtat aacagacagt aacaaagcag gtgccagctt
     4021 gcgtctctac cagaataaca ccactgaacc gggcaatccc actctgacac atggtgacaa
     4081 cggcctgcgt gacagaaaaa cagcacaaaa tgacgtgcag ttctggtacc agtactcccc
     4141 tgcggataac agcctcatca atgtaaagtc aacgttatat ctcagtgata tcactatcaa
     4201 gacaaacggt cacaacaaaa cagcagaatg gcgaaacaac agaacctccg gtgttaatgt
     4261 tgtcaacagg agccatactc tgatttttcc gggagcccat cagttaagtt atggcgccga
     4321 atattaccgt cagcagcaga agccggaagg ctctgccaca ctatatccgg aaggaaacat
     4381 tgactttaca tcgttgtatt tccaggatga aatgacaatg aaaagcaacc cggttaacat
     4441 tatcgtcggt tcccgttatg accggtacaa gagcttcaat ccccgagccg gaaaactgaa
     4501 agccgaacgc ctgtccccca gggcggcgat ttcagtctca ccaacagact ggctgatgat
     4561 gtacggctcc atatcctctg cattccgtgc ccccacaatg gcagaaatgt acagggatga
     4621 tgtacatttt taccgcaagg gtaaacccaa ttactgggta cctaacctta atctgaaacc
     4681 agaaaataac attacccgtg agattggcgc aggtattcaa ctggacggcc tgcttacaga
     4741 caatgaccgg ctgcagttga aaggcggata tttcggaacg gatgccagaa actatattgc
     4801 cacgcgcgtg gatatgaaac ggatgcgttc ttattcttat aatgtatccc gggcccgtat
     4861 ctggggatgg gatatgcagg gtaattacca gtctgattat tttgactgga tgctttctta
     4921 taaccgcacg gaaagtatgg atgccagtag cagggaatgg ctgggctccg gcaatcctga
     4981 cacactcatc agtgacatca gtatacctgt tggtcatgga ggagtttctg ccggatggcg
     5041 tgctgaactt tcggcagcag ccacgcatgt gaaaaaaggc gatccccatc aggctggcta
     5101 tgccgtacac tccttttcac tgtcttataa gcctgtaagt gttaaaggct ttgaggcatc
     5161 agtgactctg gataatgcct tcaacaagct tgccatgaat ggcaaaggtg tgccgctttc
     5221 aggcagaact gtcagtcttt atacccgtta tcagtggtaa cctcgcagga aataaatcat
     5281 gatgaaaaat acaggctata tcttagctct ttgtctgaca gcatcggagc atgccctggc
     5341 ccacgatgtc tggattaccg gtaaacaggc agagaacaac gttaccgcag agattggtta
     5401 tggtcataat ttcccctcaa agggcacaat tcctgacaga aggaatttct ttgaaaatcc
     5461 ccggctttat aacgggaaag agataataac gctgaagcca acgtccacgg attatgtcta
     5521 taaaactgag tctgcaggca acggtaatag ctacgttctg tcaacgaaaa tgaaaccggg
     5581 atactggtcg agaacctcgt caggatggaa gcctgtcagt cgggagggca gaaatgatgt
     5641 agcatactgt gagtttgtga ctaaatatgc aaaatctttt attcctggtg aacagcagat
     5701 gccagcacaa ctctatcagt ctccaacagg ggatgagctt gaaatcattc cattatccga
     5761 tatcagtcgt ttcggtgaag atgtgaagct gaaagttctg tataaaacat ccccgcttgc
     5821 cggagctact ctggagcttg actctgtcag ttatctgaaa tcatcccgtc atacccatgc
     5881 agctgagcat aaacattcgg ctcataaagc agaacttacc tttgtaagta atgaggacgg
     5941 tatcatcact gtaccttccc ttcatgtcgg gcaatggctg gctaaagtca aaaataagaa
     6001 agtatttccg gacaagagtc tgtgtgatga aactgtcgat gtggcaacct taagcttctc
     6061 ccgaaattaa taatactatt cctgccctga tttatgggca gggtattatc aatacgagaa
     6121 tagcctaccg acatccgtgt aaaaaaccac ataatgattt ttcatatcca tttccagata
     6181 tacttatgta aagatcacct ccaaa
//
LOCUS       DAZFRN010000233         6174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFRN010000233 DAZFRN010000000
VERSION     DAZFRN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.235"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            63..839
                     /gene="exuR"
                     /locus_tag="ACHKUB_004764"
     CDS             63..839
                     /gene="exuR"
                     /locus_tag="ACHKUB_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO6657669.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1079..1162
                     /gene="yqiM"
                     /locus_tag="ACHKUB_004765"
     CDS             1079..1162
                     /gene="yqiM"
                     /locus_tag="ACHKUB_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO6657670.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1184..1846
                     /gene="yqjA"
                     /locus_tag="ACHKUB_004766"
     CDS             1184..1846
                     /gene="yqjA"
                     /locus_tag="ACHKUB_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO6657671.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1850..2233
                     /gene="mzrA"
                     /locus_tag="ACHKUB_004767"
     CDS             1850..2233
                     /gene="mzrA"
                     /locus_tag="ACHKUB_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO6657672.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2380..2748
                     /gene="yqjC"
                     /locus_tag="ACHKUB_004768"
     CDS             2380..2748
                     /gene="yqjC"
                     /locus_tag="ACHKUB_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO6657673.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2786..3091
                     /gene="yqjD"
                     /locus_tag="ACHKUB_004769"
     CDS             2786..3091
                     /gene="yqjD"
                     /locus_tag="ACHKUB_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO6657674.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3094..3498
                     /gene="yqjE"
                     /locus_tag="ACHKUB_004770"
     CDS             3094..3498
                     /gene="yqjE"
                     /locus_tag="ACHKUB_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6657675.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3488..3787
                     /gene="yqjK"
                     /locus_tag="ACHKUB_004771"
     CDS             3488..3787
                     /gene="yqjK"
                     /locus_tag="ACHKUB_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO6657676.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3973..4365
                     /gene="yqjF"
                     /locus_tag="ACHKUB_004772"
     CDS             3973..4365
                     /gene="yqjF"
                     /locus_tag="ACHKUB_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6657677.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4435..5421
                     /gene="yqjG"
                     /locus_tag="ACHKUB_004773"
     CDS             4435..5421
                     /gene="yqjG"
                     /locus_tag="ACHKUB_004773"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO6657678.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5714..6079
                     /gene="yhaH"
                     /locus_tag="ACHKUB_004774"
                     /pseudo
     CDS             5714..6079
                     /gene="yhaH"
                     /locus_tag="ACHKUB_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
BASE COUNT         1543 a         1520 c         1554 g         1557 t
ORIGIN      
        1 tggcataccc atgaataaag tggtatgata acttcgtccg cattccactc cgggagacgc
       61 atatggaaat cactgaacca cgccgtttgt atcaacaact tgccgctgac ctgaaagagc
      121 gcatcgaaca gggcgtctat ctggtaggtg ataaactgcc tgcagaacgc tttattgccg
      181 atgaaaagaa cgtcagccgt acggtagttc gtgaagccat catcatgctg gaagttgaag
      241 gctatgtcga agtgcgtaaa ggttcaggta ttcatgtggt ttccaaccag ccgcgccatc
      301 agcaggcggc tgacaataat atggaattcg ccaattacgg tccgtttgag ttgcttcagg
      361 ctcgccagct catcgaaagt aatattgccg agttcgcggc aactcaggta acgaaacaag
      421 acatcatgaa actgatggcc attcaggaac aggcgcgcgg cgaacaatgc ttccgtgatt
      481 ccgagtggga tttacagttc cacattcagg tagccctggc gacgcagaac tccgccctgg
      541 cggccatcgt tgaaaaaatg tggacccaac gtagccataa cccgtactgg aaaaaactgc
      601 acgaacacat tgattcccgt accgtcgata actggtgtga tgaccacgat caaatcctca
      661 aggcgctgat tcgcaaagat cctcatgctg ctaagctggc aatgtggcag cacctggaaa
      721 acaccaagat catgttattc aacgaaacca gcgacgactt cgagttcaat gccgaccgct
      781 atctgttcgc cgaaaacccg gtagtgcatc tcgatacggc caccagtggc agtaaatgaa
      841 actcctgtcc gacaggcgct gcgacgcgcc tgtttgtctc gtcggtaagc gaaaagtata
      901 aagtgtcagc ctgtgtaaat cctctcgcca ccctcccctg cattcagcaa aatcagactc
      961 cacggacatg taattttgat aacgaacaac gttgaccttt gttacaatta gattcaattt
     1021 gaatttatgt ttttgaatgc tttcttatct cacgatttaa cagggaatag ttcaggctgt
     1081 gttgatgtat caaacccgca gaacatacca aaacagcaat aacattgcgg tagtgcatct
     1141 tttaaaacca gcgtggcgtt aaccgattca ccaggaataa tgaatggaac ttttgaccca
     1201 attgctgcaa gccctgtggg cgcaggattt tgaaaccctg gccaatccat cgatgattgg
     1261 catgttgtat tttgtcttgt ttgtaatttt gttccttgaa aacggcttgc ttccagcggc
     1321 ctttttaccg ggcgacagtt tactggtatt ggtcggcgtg ttgattgcga aaggcgcgat
     1381 gggttacccg caaacgattc tgctgctgac cgttgccgcc agcctcggct gctgggtcag
     1441 ctatattcag gggcgatggc tgggcaatac ccgcaccgta caaaactggc tatctcattt
     1501 acccgcgcat tatcatcaac gcgcacacca tctttttcat aaacacggtt tatcggcgct
     1561 gttaattggt cgctttattg cgtttgtcag aacactgctg ccgacgattg ccgggttatc
     1621 agggctgaat aacgcgcgct ttcagttttt caactggatg agcggtctgc tgtgggtatt
     1681 gatcctgaca actctgggtt acatgctcgg caaaacgccg gtatttttaa agtacgagga
     1741 ccagctgatg tcatgcctga tgctgctccc ggtggtgctg ctggtgtttg gcctggcagg
     1801 ttctctggtc gtgttatgga aaaagaaata tggaaatcgg gggtaaggga tgcaaatacc
     1861 tcgcatgtcg cttcgccagc tagcctggtc cggcactgtt ttacttctgg tcggcacgct
     1921 gttgctggcc tggtccgcgg ttcgccagca agagtctacg ctggcgattc gtgccgttca
     1981 tcaaggcaca acgatgccag acggtttttc gatctggcat caccttgacg ctcatggcat
     2041 tcctttcaaa agtatcaccc ccaaaaacga cactctgtta attacatttg actccagcga
     2101 ccagagcgcc gccgcaaaag cggtcctcga cagaacattg ccccatggct acatcattgc
     2161 gcagcaggac aataacagtc aggctatgca gtggctgacc cggttacggg ataactctca
     2221 tcgcttcgga taacttcctg ttatccgaaa catatcactc actttggtga tttcaccgta
     2281 actgtctatg attaatgagg cggtggttaa cactaccgct tcgttgtctg gacaatcgtt
     2341 cctttgtaat aggtccgaat cacaatggaa ggttcaagaa tgaaataccg catcgcttta
     2401 gctgtttctc tctttgctct tagtgccggt agttatgcca ctaccctgtg tcaggaaaag
     2461 gagcaaaata tccttaagga gatcagctat gccgaaaaac accaaaacca gaatcgtatt
     2521 gacggtctga ataaagccct gagtgaagtc cgggccaact gttcagatag ccagctgcgt
     2581 gccgatcatc acaagaaaat cgcaaagcag aaagatgagg tggcggaacg ccagcaagat
     2641 ttagccgagg cgaagcaaaa aggcgatgcc gataagattg ccaaacgcga acggaaactg
     2701 gcagaagcgc aggaagagct gaaaaagctg gaagcgcgcg actactaact cacaatagtc
     2761 actacttact cacctggaga aaactatgtc gaaagaacac actacggaac atctgcgtgc
     2821 tgagttgaaa tccctttccg atacgctgga agaggtgctt agctcatctg gcgagaagtc
     2881 gaaagaagag ttgagtaaga ttcgtagcaa agcggagcag gcactgaaac agagccgtta
     2941 tcgcctgggt gaaaccggtg atgccatcgc caaacaaacc cgtgtcgcgg cggcgcgtgc
     3001 tgatgagtat gtgcgtgaaa atccgtggac cggcgtgggc attggcgctg caatcggtgt
     3061 agtgctcggc gttctgctgt cgcgtcgtta attatggcgg acactcatca cgcgcaaggg
     3121 cccggtaaaa gcgttctggg catcgggcag cgaattgttt ccatcatggt tgaaatggtg
     3181 gaaacacgtc tgcggctggc ggtggtggag ctggaagagg aaaaagctaa tctctttcaa
     3241 cttttactga tgctgggcct gacgatgctt ttcgctgcat ttggtcttat gagcctgatg
     3301 gtgctaatta tttgggcggt tgatccgcaa tatcgcctga atgcgatgat tgccaccacc
     3361 gtggtgttgc tgctactggc actgattggc ggtatctgga cgttacgtaa atcgcgtaaa
     3421 tccaccttgc tgcgccatac acgccatgag ctggcaaacg atcggcaact gctcgaggag
     3481 gagtcccgtg agcagtaaag tcgaacgtga acgacgtaag gcgcaactgc ttagccagat
     3541 ccagcaacaa cggctggatc tttccgccag tcgtcgtgaa tggctggagg caacaggcgc
     3601 ttacgatcgt cgctggaata tgctgctaag tctgcgctcc tgggcgctgg ttggcagtag
     3661 cgtgatggcg atctggacga ttcgccatcc taatatgctg gtccgctggg ccagacgcgg
     3721 ttttggcgta tggagcgcct ggcgtctggt taaaacgacc ctcaagcagc aacagcttcg
     3781 cggttaactt ccctctggcc ggagcaattc cggccttatc cctcaaattt tttgaagatt
     3841 tttgacagtt ttccttgcta acaatcatca ttcaccacgt ttatgattct ctccatcgac
     3901 agcaacgacg ctaataccgc gcccttgcac aaaaaaacaa tcagcagcct gagtggcgca
     3961 gtggagagta tgatgaaaaa attagaagat gttggtgtac tggtagcgcg cattttaatg
     4021 ccgattctgt ttattaccgc aggctgggga aaaatcaccg cttactctgg tacccaacaa
     4081 tatatggaag caatgggtgt cccgggtttt atgctgccgc tggtgattct gcttgagttc
     4141 ggtggtggtc tggcaatcct gttcggtttc ctgactcgca ccacagccct gtttactgcg
     4201 ggctttacgc tgctgacggc atttttattt cacagcaact ttgctgaagg cgtcaactcg
     4261 ctgatgttca tgaaaaacct gacaatttct ggcggattcc tgctgctggc aattaccggt
     4321 ccgggcgcgt atagcatcga ccgcctgctg aataaaaagt ggtaagcacg cgctatactt
     4381 aacgataaaa aacgaggagg aagctcctcg tttttgctat tggaggagag aaaaatgggt
     4441 caactgattg acggcgtctg gcatgacacc tggtacgata ccaaatctac cggcggtaaa
     4501 tttcaacgtt cagcttccgc atttcgtaac tggctcactg ccgatggcgc acctggcccc
     4561 actggcacag gcggttttat cgcagagaaa gatcgttatc atctctatat ttcactcgcc
     4621 tgcccgtggg cgcaccgcac gctgatcatg cgcaaactca aaggactgga accgtttatt
     4681 tccgtttccg tagtgaaccc gctgatgctg gaaaacggct ggacctttga tgacagtttt
     4741 ccgggagcaa ccggagatac gctctatcaa catgaatttc tgtatcagct ttatctccac
     4801 gccgatccac actacagcgg acgagttact gttcccgtgc tgtgggacaa aaagaaccac
     4861 accatcgtca gcaacgaatc agcagaaatc atccgcatgt tcaataccgc gtttgatgcg
     4921 ctgggcgcaa aagcgggtga ttactaccca ccagccctgc aaacgaaaat tgacgaactt
     4981 aacggctgga tttatgacac cgttaacaac ggcgtgtata aagctggttt tgccaccagc
     5041 cagcaagcct acgacgaggc ggtggcgaaa gtgtttgaat cgctggcgcg actggagcag
     5101 attttaggcc agcatcgtta cctgaccggc aaccagctaa ccgaagccga tattcgcctg
     5161 tggactacgc tggtgcgttt tgatccagtg tatgtgaccc acttcaagtg tgataagcac
     5221 cgcatcagcg attacctgaa tctgtatggc ttcctgcgcg atatctacca gatgccgggg
     5281 atcgccgaaa ctgtcaattt cgatcacatc cgtaatcatt acttccgcag ccataagacc
     5341 atcaacccta cggggattat ttcaattggt ccgtggcagg atctcgatga accgcatgga
     5401 cgagatgttc gcttcggtta aaaaaaggca ccttacgagg tgcctctgat aattagctaa
     5461 gtgcatgttg ttcatgtcgg atgcggcgtg aacgccttat caaacctaca aaacatttat
     5521 attcaatatg ttgcaatgac cgcgcaggcc agataagcgt agcgcatctg gcgattttgc
     5581 gcttgtcatc agtcttagcg cgccttcaca gcactttatt aattccttga acgaatattt
     5641 actgccattt cattcacgtc tattcttaat ttgctgcttt aaaaacaagt gattgagcaa
     5701 aattgaggca aaaatggact ggtatctgaa agtactaaaa aattatgtcg gtttccgggg
     5761 tcgtgcgcgg cgcaaagagt actggatgtt tattctggtc aacatcatct ttacgttcgt
     5821 gctggggctg ctggataaaa tgttaggctg gcaacgcgct ggtggcgaag gcatcctgac
     5881 gacaatctac ggtattctgg tgtttttacc gtggtgggca gttcagttcc gccgcctgca
     5941 cgacaccgac cgctcggcgt ggtaggcact gttgttctta atcccgttta tcggctggct
     6001 tattatcatc gtctttaact gccaggcggg tacgccaggc gaaaaccgct ttggaccaga
     6061 tccgaaactg gaaccttaaa acaaatctgc tcgccggatg tctgactcat ccggctttta
     6121 tcatcatgtt gatgaatgaa taatttacta atgtttattt aaaatatttc aatg
//
LOCUS       DAZFRN010000234         6058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFRN010000234 DAZFRN010000000
VERSION     DAZFRN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.236"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..725
                     /locus_tag="ACHKUB_004775"
     CDS             <1..725
                     /locus_tag="ACHKUB_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6657679.1"
                     /translation="LLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEK
                     TNIPTINVVGSPFSPAQITIETAFFEASHKLTEHMLEQGYKNIGFIGAHMDNRLQRQQ
                     LNGWHKAMLSYYQNSDLVITMPGAASLQLGRYALNEILLRQPELDAVICSHEEIALGI
                     MFECQRRLLKIPGNIAVACLDGSDSCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNN
                     HDESEESRIVQFNYQIELRQST"
     gene            928..1608
                     /locus_tag="ACHKUB_004776"
                     /pseudo
     CDS             928..1608
                     /locus_tag="ACHKUB_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1625..1933
                     /locus_tag="ACHKUB_004777"
                     /pseudo
     CDS             1625..1933
                     /locus_tag="ACHKUB_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2036..3142)
                     /locus_tag="ACHKUB_004778"
                     /pseudo
     CDS             complement(2036..3142)
                     /locus_tag="ACHKUB_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3908..5956
                     /gene="ireA"
                     /locus_tag="ACHKUB_004779"
     CDS             3908..5956
                     /gene="ireA"
                     /locus_tag="ACHKUB_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO6657680.1"
                     /translation="MKNKYIIAPGIAVICSTVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
BASE COUNT         1724 a         1333 c         1402 g         1599 t
ORIGIN      
        1 tactgcttgg gtgtcatgag tataatcagc tcaaagaagc tgaaatcctg atgacgcttc
       61 ttcaaggtaa cccggcggcg ttagttattt ttggttctca gttggctgat aaaactcacc
      121 agatactcga gaaaacaaat atacctacca ttaacgttgt cggttcccct ttcagtccgg
      181 ctcaaattac catcgaaaca gcattttttg aagcatcaca taaactgacc gaacatatgc
      241 tggaacaggg ttataagaat atcggattta tcggtgcgca tatggacaac cgcctgcaac
      301 gtcagcaact gaacggctgg cataaagcaa tgctgagcta ttaccagaac tctgatcttg
      361 ttatcaccat gccgggtgcc gcaagtttgc aattggggcg ttacgcatta aacgagatat
      421 tactgcgaca accagagctg gatgctgtga tttgcagcca cgaagagatt gcgctgggta
      481 tcatgtttga gtgccagcgg cgtttgttaa aaatccctgg aaatatcgct gtagcttgtc
      541 ttgacggttc agacagttgc gatcaaacac acccaacctt gacatccatc cgtattgact
      601 acaaaaaaat ggggacagag acgggtaagc tgctgattgg actattgaat aataatcatg
      661 atgaatcaga agagtcgcgt atcgtgcaat tcaactacca gatagaatta cgtcagagta
      721 catgagctca aaaggggatt attgcaaagt tagctattgt ggtagggata gccgtttccg
      781 gctattcccc gcgcagagcc ggatgtgtgc tgctaacgca tcctgtcaac gacggatgaa
      841 aagttatcca cttatatctc caccaacggc ccaatattgt accgttcgtg acacatccat
      901 ttctacttca ggtatttggc gtagtagcgg gtgctaaaaa tttgccgcaa aaccgcaata
      961 tcagcccgca gaggcgctct ccagattatc cattcctata ttatctctgc accggatgaa
     1021 ttactcgaac aactcagaaa tatgacacat atgcaactta tcaggactct gggatcctgg
     1081 aggcctgatg ccagtgaata cagtaatgtt accaacgttt atcgcattac attgaaatcc
     1141 cttgttcgac gctatttcga gtttcatgat gaaatcacca acttggatgt catgattgcg
     1201 gcaattgttg atgaactggc acctgaactg attaaacgta atgctgttgg atacgaaagc
     1261 gctttgcagt tgttgatctc tgccggagac aatccctaac ggttaagaac cgaatcaggt
     1321 tttgcggcgt tgtgcggtgt cagccagttt cccctatctt ctggaaaaac gaaccattac
     1381 cgacttaatc ggggtgggga gcgtgttgca aatagtgcac tttacatcat cactatagga
     1441 tgtttacaaa ctgacgataa aacaaaggaa tatgtctcca ggcgagtagc cgaaggacat
     1501 acaaaaatgg aagcaatacg ctgtctgaag cgctatatct cacgcgaagt ttatacatta
     1561 ctacgaaatc aaaacaggca tgttaactgc atcccgataa cgtcttgact cttagaaggg
     1621 cgcccaaggc agtcattatt cgagcaggca gttccggcag ttattgtgtc gataccagat
     1681 caggcagagt atgagccggc gcggaaaatg ctgggataac agctcaatgg aacgcttctt
     1741 caggagtctg aagaacgaat ggatgccggt ggtgggttac gtaagcttca gcgaggcagc
     1801 tcactccata acggacgata tcgttggata ttacagcgca ctaagaccgc acgaatataa
     1861 cagtgggtta tccccaaacg aatcggaaaa tcgatactgg aaaaactcta actcggtggc
     1921 cagtttttgt tgaccacttc agccatcgcc agacgctaac atgagttact cttgttctga
     1981 gctacgtcac cttcagggaa ctggttatcc tcattcttta tttgtgacat tgtggttatt
     2041 gtgcgacaaa cgtggctatc ccgccccata tcccagataa aaccacctgc tcacgtgcga
     2101 cagcgacaat tgtaatattg acattctttc ctttggcctg aagttttcga gacctcttgc
     2161 aaagcctgag ttgagcatcc catgctctgt caatgacggg gcggggtaaa ttttcctgat
     2221 tttttgtatt gccggactga tgcgtgtcgg atgccggtaa ctccacgctg cctcaaccaa
     2281 tagctttctg gcatagctgt ttccggcttt ggtaatgctg ccctgatgtc tgcttccacc
     2341 cgatgaatat tctgatggcg tgataccaaa ccatgacatc agttgtcgtg gatgttcaaa
     2401 acgggtcaga tcacccagtt cagaaattaa tccaaccgca gtaataaact gaatccctcg
     2461 catggcttgc tgcgcctcta ctaccggata cagcctccat tcggtaacgg cctccttcgg
     2521 gcggattcca gccgttcaca ttgtgcctgc cggtcttcta ttgtacaacg gtgttcgtca
     2581 aaagctaatt gtcgccaggg actttcgaat gagtatttgc tgagccagtg tcggtgtgca
     2641 gggccccaat ctgctcgccc gacataatgg accccatgaa ccagaagaaa cgatttcaga
     2701 cgttgccttg catgccgtaa atcatcgcgg gcagatgccc atgcctgggc caaatcccgg
     2761 aatgcctcat cttcaacgcc ggatacgtag acagcagaaa gatctcctgc acgcagtgaa
     2821 cgcacaagtc tgatggcatc acggtgtcgg ttttaccccg ctcccccggt tttttcggga
     2881 taagtgaggg ggcgcagacc atgcagtcaa aaccggattt caccagccga cgataaagtc
     2941 cataaccaca gggtcccgct ttataaacga tactgacctg cgatgacttt gacctgatac
     3001 gttgataaag attatgaatg tcagtaggtg atgtgctaat tttacccatc agttcaacag
     3061 gttctgaatt gatagcatag gagacagtaa tcgattcttt gtgaacatct aattcgacgt
     3121 acaacatgtt attgttgttc atgctggcct ccgtggtgga aattccaggg gggtagcaac
     3181 agctcaccca tgtggctctg acaacgatgt taacccgcat taaatatccc acgttggaca
     3241 gcacctgtct cacggaaagt catactgcct tacgtgcata tttcacctta cgccttcact
     3301 gtctgtcatc ggaagtactg atttatattg gggctttgca cttactgtca ttcacgggaa
     3361 aagatggtgg tgaacagtac gatactgtct tggctgttgc cgagggaaga ttcaggagtg
     3421 gtaagggggt tagtggtgta agatgagtct ggagctctga ctggtcagtg acgcgaaact
     3481 caatctgcca ggataaactc ccccttgctt ttgcgcagaa gcagtgggcc ttggttcgct
     3541 tatgtcctgc atctacaagc gaatgactga ccggagactc ccatatctgg tcagtcgccc
     3601 atttttattt tccaccacat cggacaagca taccgtaata aacagcacca gtctcctgtt
     3661 cactcttgct tgatttactg gaatgctgtt tatggagata tccggttgaa agattttacc
     3721 tcttcgacag gactataaat tatacagtaa ggagtccctc gtagaaaggg gctgattctc
     3781 atcaagtcgg acaggttcaa agtgttacct gaatgagcta aatgttattt accttccttt
     3841 ctgttcattg taaatttttc ttgctaacgc aaatcattat cattacgatt ttagaaaagg
     3901 gaaatttatg aagaacaaat atatcattgc tccgggcatt gccgtgatat gttctacagt
     3961 catatcatca ggttatgcca gttctgataa aaaagaagat acgcttgttg ttactgcctc
     4021 cggcttcact cagcaactca gaaatgcacc ggccagtgtt tcagtcatta cttcagagca
     4081 actgcaaaaa aaaccggttt cagacctggt cgatgcggta aaagatgttg aagggattag
     4141 tatcaccggt gggaatgaaa aaccggatat cagtatacgt gggttaagtg gcgattatac
     4201 attgattctg gtcgatggac gacgtcagag tggtcgggaa tccagaccaa acggcagcgg
     4261 cggttttgaa gccggattta tccctcctgt ggaagcaatt gagcgtattg aagttatacg
     4321 tggccctatg tcttccctgt atggttccga tgctatcgga ggagttatta atatcataac
     4381 caaacctgtt aataaccaga catgggatgg cgtacttggg cttgggggaa ttattcagga
     4441 acatgggaaa tttggtaact caaccacgaa tgacttctac ctgtcaggcc cattggttaa
     4501 ggataaactt ggtcttcagt tatatggagg aatgaactat cgcaaggaag atagtatctc
     4561 tcagggaaca ccagcaaaag ataataagaa tataacggca acactccagt ttactccgac
     4621 tgaaagccag aagtttgttt ttgaatacgg aaaaaataac caggaacata cattaacacc
     4681 tggtgattct cttgatgcct ggactatgcg gggaaatctt aaacaaccaa acagtaaaag
     4741 agaaacacat aactcacgta gtcactgggt agcagcatgg aatgcccagg gcgaaatact
     4801 gcatcctgaa attgctgttt atcaggagaa agttattcgt gaggttaaat ctggtaaaaa
     4861 agataaatat aatcattggg atcttaatta cgagtcaaga aaaccggaaa taaccaacac
     4921 gatcatagat gcaaaagtga cggcatttct gccggaaaat gtgctgacca tcggaggtca
     4981 atttcagcat gcagaactcc gtgatgactc agccacaggt aaaaaaacga cagaaacaca
     5041 gtctgtttca gttaaacaga aagctgtttt tatagaaaat gaatatgctg caacggattc
     5101 tctcgccctg actggagggc tgcgcctcga taaccatgaa atctatggca gttactggaa
     5161 tccaagattg tatgctgttt ataacctgac cgataatctc actctcaaag gagggatcgc
     5221 aaaagcattt cgggcccctt cgattcgtga ggtcagtcct ggatttggaa cactgacgca
     5281 gggtggtgcc tctattatgt atggaaacag ggacctgaaa ccggagacca gtgtaaccga
     5341 agagatcggt attatttata ataatgacag tggtttttca gcaagcgcga ctctgtttaa
     5401 tacagatttt aaaaataagc tgaccagtta cgatataggc acaaaagatc cagtcactgg
     5461 gctaaacact tttatttatg ataatgtggg tgaggcaaat atcagaggtg tggagcttgc
     5521 aactcagatt cctgtgtatg ataaatggcg tgtatctgca aactatacat tcactgactc
     5581 ccgtcgagaa agcgatgacg aaagtctcaa tggcaagtcg ctgaaagggg aacctctgga
     5641 aagaactccc agacatgcag ccaatgcaaa actggaatgg gattacactc aggatattac
     5701 attttattca tctctgaatt atacaggaaa acaaatctgg gcagcacaaa gaaatggtgc
     5761 caaggttccc cgtgttcgta atggattcac ttctatggat attggtatga attaccagtt
     5821 cctgccaggc acgctgatta attttgccgt acttaacgtc acagacagaa agagcgaaga
     5881 tatcgatacc attgatggta actggcaggt cgatgaagga cgtcgttact gggctaatgt
     5941 aagaatatcc ttctgagaga atatagataa cctgaggccg tgcgcctcag gtaccagtat
     6001 agtaatcagt aacgtcagct tccaggttgt aatgctttta attttgataa aaataaac
//
LOCUS       DAZFRN010000235         6053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFRN010000235 DAZFRN010000000
VERSION     DAZFRN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.237"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(301..1260)
                     /gene="nrdF"
                     /locus_tag="ACHKUB_004780"
     CDS             complement(301..1260)
                     /gene="nrdF"
                     /locus_tag="ACHKUB_004780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6657681.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1270..3414)
                     /gene="nrdE"
                     /locus_tag="ACHKUB_004781"
     CDS             complement(1270..3414)
                     /gene="nrdE"
                     /locus_tag="ACHKUB_004781"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6657682.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASSEYFSQYLQGNWQPKTTKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3387..3797)
                     /gene="nrdI"
                     /locus_tag="ACHKUB_004782"
     CDS             complement(3387..3797)
                     /gene="nrdI"
                     /locus_tag="ACHKUB_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO6657683.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3794..4039)
                     /gene="nrdH"
                     /locus_tag="ACHKUB_004783"
     CDS             complement(3794..4039)
                     /gene="nrdH"
                     /locus_tag="ACHKUB_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO6657684.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4287..4616)
                     /locus_tag="ACHKUB_004784"
     CDS             complement(4287..4616)
                     /locus_tag="ACHKUB_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO6657685.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4768..5112
                     /locus_tag="ACHKUB_004785"
     CDS             4768..5112
                     /locus_tag="ACHKUB_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO6657686.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5149..5598)
                     /gene="alaE"
                     /locus_tag="ACHKUB_004786"
     CDS             complement(5149..5598)
                     /gene="alaE"
                     /locus_tag="ACHKUB_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO6657687.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1557 a         1456 c         1489 g         1551 t
ORIGIN      
        1 gtcacttttt tctaccctaa catactgaga atctgaggca acccctgagg gcaaatgtgg
       61 catgaatatt gatgcgagca aatcgccgga atttcccgtg atataagggc tgagagcaaa
      121 tcgagaaaag ttgatatttt ttgtcataga tagcgaaaaa acgcctgaat attgtagttt
      181 caggcgaata atgcaacaga gtatcagtgt agatcactac agaatatttg cgtgataaaa
      241 agctatttgg cgggaattat ttccctgctg cgggtagtga tatttttgaa aataacaccc
      301 tcagaaattc cagtcttcat cttctgtttc aaccgctttc cccatcacat aagaggagcc
      361 tgaaccggaa aagaaatcgt gattttcatc ggcattcggc gaaagcgcgg cgaggattgc
      421 cggattcact tccgccattt ctgcgggaaa taacggttcg tagcccagat tcatcaaagc
      481 cttattggcg ttgtaacaga gaaacgcttt cacatcgtca gcccacgggg tttcggcgta
      541 cagctcatcg gtgtattgca actcgttgtc gtagagttcc agcaacaaat cgaaggcgaa
      601 actcttcaac tcttcacgtt gtcccagaga tatcttttcc atgtttttct gatatttata
      661 gcctatgtag taaccgtgga ctgcttcatc gcggataatc agacggatca gatctgcggt
      721 attggtcagc tttccgcggc tggaaaaata catcggcagc cagaaaccgg aatagaacaa
      781 aaaagattca agaaacacac tggcgatttt ctttttcagc ggatcatcac cgcgataatg
      841 ttgctgaata atctgagctt ttcgctgcaa cggtgcgttt tcttcactcc aggcgtaggc
      901 ggcatcgaca tctttggtct gacacagcgt cgagaaaatc gaactgtaag agcgggcatg
      961 aaccgcttcc ataaagctga tattcgataa taccgcttct tcatgaggcg tgagtgcatc
     1021 gggcatcagc gaaggcgcgc cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt
     1081 aaaaacgcgc atcgtcagtt gttgttctac gacagttaat gtttgccagg caggaatatc
     1141 gttcgacagc ggcacctttt ctggtagcca gaaattgctg gtcaggcgat tccacacctc
     1201 cagatcttta tcgtcagata tcttgttcca gttgatggcg ctgatacgtg agagtttcat
     1261 agatattcct taaagtgcac aggagacgca gccttcaatt tcagtgcctt ccagcgccat
     1321 ctgacgcagg cgaatgtaat agagcgtttt gatacctttg cgccaggcgt aaatctgcgc
     1381 tttgttgata tcgcgagtgg tggcggtatc ggggaaaaac agcgtcagcg acaaaccctg
     1441 atcgacatgg cgagtcgctt ccgcgtaggt gtcgatgatc ttttctgcgc cgatttcgta
     1501 agcatcctga tacagcgcca gattctcgtt agtcataaac ggggcagggt agtaaacgcg
     1561 tcctgttttg ccctctttgc gtatctctac tttcgccaca atcggatgaa tactcgacgt
     1621 agcatggttg atataagaga tagaaccggt tggcggcacc gcctgaagat tctggttata
     1681 aatgccgtag cgcatcacgt cgtcgcgcag ttgcgcccac atctcacggg taggtaacgt
     1741 aataccgcta cgggcaaaca gttcgccaac tttcgtcgtt ttcggctgcc agttcccttg
     1801 cagatattgg ctgaaatatt cactactggc atagcgtgac tgtttgaacc cggcgaaggt
     1861 ttcaccgcgt tcgcgtgcca gcaacatcga ggtacgcagt gcgtgccagg tgatggtata
     1921 gaaatagaga ttggtgaaat ccagtgcttc cggcgaacca taagcgatgc cttctcgcgc
     1981 cagataaccg tgtaaattca tctgccccag cccgatggcg tgtgaggcgg catttccggc
     2041 ttcgatggac ggcacgctgc ggatatgact catatctgac accgccgtta aaccgcgtac
     2101 ggcagtctct accgtgcggg caaagttggg ggaatccatg gtgtgggcaa tattcaacga
     2161 acctaaattg caggaaatat catggcctgt acgggcatag tcgagattct cgtcatactc
     2221 tgaggcgctg ttaacctgca aaatttctga gcatagatta ctcatattta tgcgcccggc
     2281 gatagggtta gcacggttta ccgtgtcttc atacatgatg tagggatagc cggactcaaa
     2341 ctggatttct gccagtcgct ggaagaaatc acgggcgttg aggtattttt tgcggatgcg
     2401 ttcatcggca accagttcgt catagtgttc gctaatggcg atatcggcaa acggcttgcc
     2461 ataaactcgc tcaacgtcat agggcgaaaa cagcgccatc tgcgcattct ctttggccag
     2521 atggaaagtg atatccggga tcaccacgcc aagcgacagt gttttaatgc ggattttttc
     2581 gtcggcattt tcccgtttcg tgtcgagaaa acgcagaata tcgggatgat gagcatgtaa
     2641 atagactgca ccagccccct gacgagcgcc gagttggttg gcataggaaa atgcgtcttc
     2701 cagcattttc atcaccggaa ttacgccaga agattgattt tcaatacgtt taattggcgc
     2761 gcccgcttcc cgcagattcg acagcaaaaa tgctacgccg ccgccgcgtt tcgacagttg
     2821 cagtgcggaa tttaccgccc gaccaatcga ctccatattg tcttcaatac gcagcaaaaa
     2881 acaggaaacc agttcgccgc gctgctgctt accgcagttg aggaatgtcg gcgtggctgg
     2941 ctgaaagcgt cctgacagca tttcatcggt cagttgcaac gccagcgtcg catcgccttg
     3001 tgccagcgtc agcgccacca tcgtgactcg atcggcaaaa tcttccagat aacgtttacc
     3061 gtcgaatgtc ttcaacgtat agctggtgta aaacttccat gccccgagga atgtctggaa
     3121 acgaaaaccg ctggtgtgtg cgtgggcaaa cagcgtaatg acaaagtcgc gagagtagcg
     3181 attaagaacg ctttcatcat agtaaccttc gttgaccagc cagttcaggc gctgctgctg
     3241 gctactgaag gtcacactgt tcggacgcac atgcgtcgca ataaaggcgt caacggcctg
     3301 gcggtcttta tcgaactgaa tgcgacctgc gctatcgtaa agattaagca tcgcattcag
     3361 cgcgtggtaa tccatcgttt cctgcgtcag gcattctgcg gttgtcgttg ccaaaattcg
     3421 gttactcctt tacgaacgtt ttcgatatcg ctttgcgtac ccatgagttc aaaacggtac
     3481 agccacggca cgccgcattt ctgagcaatc acatctccgg cacggccata cgcctcacca
     3541 aagttgcgat tacccgaagc aataacgccg cgaagcaacg cccggttgtg ctcgtcgttt
     3601 aaaaagcgaa ttacctgtcg tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc
     3661 acgatcagga tgtaaggctc gtctacctga atccgttccc gctcattgag cgggatgcgc
     3721 accgcgggca gacctaaacg ttcgataaaa cgctgcgtat tttcggagct gctggagaag
     3781 tagacgagct ggctcatgca ctggccgcgt gtggcgctgg atgcagacgg ttaatcatgt
     3841 ccggacggaa accagaccag ctaagatcgc cagcaatcac taccggcaac tgacgaaagc
     3901 cctgagcacg caacgcttcc gctgcttcag gaacgcgatc gacattaatc atttcaaaat
     3961 caaagccccg gttttccatc gcccgtttgg tggcgtggca ctgaacgcaa tcgttacgag
     4021 tgtaaatagt aatgcgcatg attcgtattt ccgtttaaaa tgaagatatg gcgcgatgat
     4081 acgcgtcggg ttgtctctct gttgatacag agatactaga tgtagttgaa aaaagattca
     4141 accacacaat atatagcaaa tagtggtcga aattaccctg gatatgagcg tgacggggta
     4201 gggggatttt tgtgattcac caggcaaaaa gaaaccccga agacaggctt cggggtcaaa
     4261 gacgcgtatt tattatcatt tttgcactac gatttgcgca tgcttaacag tgcgccgata
     4321 aaaataccta ccgcagctgc tgtacccacg ctacaccagg gtctttcacg aacaaaagaa
     4381 tccgcgcagc caacggcatc gcgcgcggct tgctggacgc gagtacgacc atgcattcgc
     4441 gccctggttt ctttcagcaa cgcctgagct ttactgcgtg cggcttcagc ttcccctttg
     4501 gcgtcgctgc cccaggactt caatacagat tcgaggctgt cagctaattg attgacatca
     4561 ttctggatat cctgcacgcc atcatcaacg tcgttgcggt tcggtcggtt aaacatatga
     4621 tcccccatta gaattcgatg taattttcag tgtagaccat aattttctta actgaggttc
     4681 atcgcggctt tatggacttt tctgaaagcg ttgtgaatgc gcaatcaggt aaggtaggcc
     4741 cgcggttgat aataacgagg atcaaatatg tatttacgac cagacgaggt ggcgcgcgta
     4801 cttgaaaaag tcggttttac tgtcgatgtg gtaacccaga aagcgtatgg ttaccgccgt
     4861 ggggaaaatt atgtctatgt taatcgcgaa gcgcgaatgg gacgtaccgc tctggtaatt
     4921 catccaacat taaaagaacg cagttcaaca ctggctgaac ccgcttccga tattaaaact
     4981 tgcgatcatt accagcaatt tccgctctat ttagcaggcg agcgacacga gcattacggt
     5041 atcccgcatg gcttcagttc gcgtgttgcg cttgaacgtt atttgaatgg tttatttggc
     5101 gaagccagtt aaagacgcga ctggcgatgc cagtcgcgaa aagaagagtc aggcttttac
     5161 ctgctggtaa cggctgactt taaacagtcg gcggcaataa tcgaggaagt agccataaac
     5221 cgcccccatc aacatcgaaa caacgatgtt tgaactgacc gccgccataa tctgatgcca
     5281 gtctgcgccc accactaaca agatcgccac atacaccggt gactggaacg tcacataagc
     5341 caggatatca gccagatttt ttatccagcc cgacgggcta actttgcgtg ccgcgcgcat
     5401 aaacagatcg cggtacatac cgtatggcca tgcaattaag atgttcaccg gaatcgctac
     5461 caatctggaa taaaaagact gttcgaagct cattccggag aggaaaactt caatacacat
     5521 gttcacgaca gaacagtaaa caaccatcgc gaacgtatct gcaactgcat gacgcaagcg
     5581 tgactgcggt gagaacatgc taatgctcct taataaaagc gtaatgggat gttaatggag
     5641 atgaaagcag tggatctcat tgtcatctgc attttcgcat agcgtatatc gctggctatg
     5701 actattcaat tagtgaaaaa tttttattta gtcagttttt gtagataaaa tattccgtaa
     5761 cggctcgttt tttgtacatt gtggattttt acatgccgat atgatttatt ttcaattaaa
     5821 tcaattagat atgaccttgc ctttaatttc actaatgaaa gtttgggggt gaggtaacgc
     5881 tataagcgta gagaattcgg tgtatgtcgt gaccgtgtca tttacagcat tttttatgcc
     5941 agaaaatgta cattaacagc agaaagcacc agtgaggggt gttttctttt attctgttat
     6001 attgcatttt tattcacttc ggttttgtca ggcttgcgga attagcgagc aga
//
LOCUS       DAZFRN010000236         5973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFRN010000236 DAZFRN010000000
VERSION     DAZFRN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.238"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..651)
                     /locus_tag="ACHKUB_004787"
     CDS             complement(<1..651)
                     /locus_tag="ACHKUB_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657688.1"
                     /translation="MLEQTVKNINSRFGWRNTRKLLGSSLGVTAQGLPLFIERVNSVV
                     QHNPDLKDRIDDFWKGLIFSGNRLLSIYRITDEDVAKLQTIFTNQKKDNSPFSEKYPT
                     PLSREELLVADTELHFAELRQDIIRDKQIDTAVFLSKAYYTEVIELDPTHLSDAGMEL
                     RANGGEIKCKTRQVTQCFNTIMLMPAEKILILTIDLSILPRSESQPQQYLVAKFIKK"
     gene            998..1153
                     /gene="hokD"
                     /locus_tag="ACHKUB_004788"
     CDS             998..1153
                     /gene="hokD"
                     /locus_tag="ACHKUB_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6657689.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1346..2458)
                     /locus_tag="ACHKUB_004789"
     CDS             complement(1346..2458)
                     /locus_tag="ACHKUB_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HFO6657690.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
     gene            2668..2946
                     /locus_tag="ACHKUB_004790"
     CDS             2668..2946
                     /locus_tag="ACHKUB_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657691.1"
                     /translation="MAHDTKLHNSDNSAVFASRRRRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2948..3997
                     /locus_tag="ACHKUB_004791"
     CDS             2948..3997
                     /locus_tag="ACHKUB_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6657692.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQVEL
                     IFRFLDHAFATGVLG"
     gene            4010..4372
                     /locus_tag="ACHKUB_004792"
     CDS             4010..4372
                     /locus_tag="ACHKUB_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6657693.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLEIRITEVGVHE"
     gene            4365..5054
                     /locus_tag="ACHKUB_004793"
     CDS             4365..5054
                     /locus_tag="ACHKUB_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO6657694.1"
                     /translation="MNNQYLQFVREQLMIATADLSVSIKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIALVSPVEFSTSSWRRAVLELEAHQK
                     AWLLWCYGGNICWEYQIAITQWAWNEFNTQSGTRKIAGKTLERLKKLIWLAAQAVKAE
                     LFGGEGYEYRDLASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQNIAKLD"
     gene            5287..5649
                     /locus_tag="ACHKUB_004794"
     CDS             5287..5649
                     /locus_tag="ACHKUB_004794"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO6657695.1"
                     /translation="MCDFRGMVVPEIVKVRPVVVVSRNRHNNQLVTVVPISTTEPIPR
                     RDCHHELSENPIPGNEHITCWVKCDMLMTVSLSRLDRIKTRTWEGRNYIVPMIAEDEF
                     ENIKRAVLHGIGMAYLYR"
     gene            5775..>5973
                     /locus_tag="ACHKUB_004795"
     CDS             5775..>5973
                     /locus_tag="ACHKUB_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657696.1"
                     /translation="MMTSLLPLCKRHLNGAFVVFIKRSPPLAGFFMPEKRHRALNALV
                     VANTGLSACWLFRQELLVCHVN"
BASE COUNT         1555 a         1378 c         1500 g         1540 t
ORIGIN      
        1 tttttttata aatttagcca caaggtattg ttgaggctgt gattcacttc ttggaaggat
       61 agataaatca atcgtaagaa ttaatatttt ttctgcaggc attagcatga tggtattaaa
      121 gcattgcgtg acttggcgtg ttttgcattt tatttctcca ccattagcac gtaattccat
      181 acccgcatcg ctaagatgtg ttgggtcaag ctctatcact tcagtgtaat acgctttgga
      241 taaaaaaaca gcagtgtcaa tttgtttatc acgaatgata tcttgacgta gttcagcaaa
      301 atgcagctca gtatcagcaa ctaacagctc ctctctggat aaaggagttg ggtatttttc
      361 tgagaagggg ctattgtcct ttttctgatt tgtgaaaatt gtttgtaatt ttgctacgtc
      421 ttcatcggtg attctataaa tagaaagtaa tcgattcccg ctaaaaatta aacctttcca
      481 gaaatcgtcg attctgtctt ttaggtcggg attatgctga actacactat ttacacgttc
      541 aataaaaagg ggtaatcctt gggccgttac acccagtgat gaaccaagaa gcttacgggt
      601 gttgcgccag ccaaagcgtg aattgatgtt ttttactgtt tgctcaagca ttaacacttc
      661 cttaactttc ccttaacaat ttagggtgct aaactttttt gcgcagttta cataaaaaaa
      721 tagtgcgtac gcaaaaacta tctaacatga tgagctttaa ccgggaaatt tgtatatgtt
      781 gtgagcataa agttggctga gaaatctaaa acgggtagaa tgactgcggg tgcttgaggc
      841 tatctgtctc aggcatgaac accaaaaggc agatagagaa aagccccagt taacattacg
      901 cgtccggcaa gacgcttaac attaatctga ggccatatct atgctctaca cacgtaggtt
      961 agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa aggcgatgct
     1021 aatcgccctg atcgtcatct gtttaaccgt catagtgacg gcactggtaa cgaggaaaga
     1081 cctctgcgag gtacgaatcc gaaccggcca gacggaggtc gctgtcttca cagcttacga
     1141 acctgaggag taagagacca ggcgggggag aatcccataa gcgctaactt aagggttgaa
     1201 ccatctgaag aatgcgacgc ctcggtgcct cgttaagacg atgcctcgcg ttcttcaatt
     1261 gcgttttgta ggctgtcagg gatactgtcc cacgaatggc cactgtaagc tccagatgac
     1321 catttttgtt attctccaca acgagttagt tcttcttttc ggatccggca cttctggggg
     1381 ggaaatccag cgatggctgg attatgtcgt caattaaaaa tgcggcgagt agattagcaa
     1441 atatccacgc tttcgcgagt tcaggttcct ttgcacgcaa agcatccagg tgcagcaaac
     1501 ttttgagccg cttaaaagcc agttcaattt gccatcgcag acggtaacaa tcagccactt
     1561 gctctgctga atattcatct tccggtaatg atgttagcaa tagcacatgg cccgctgctt
     1621 ccagcgtttc cgcctgaact actcgtcctt ttcgacgatt ctcgctgagc agtcgggttt
     1681 tactgattaa tgctttttcg ggaggaagtg atacggcaat gagacgtgcc ggaaagggag
     1741 ctccggcttt tttattacct gaattgccta tcattacagt ggtttcaccg ttcttaccgc
     1801 aatccagccc gcgcagaaaa cccatcatgt caaagcgcat tccttctgca gttaaccagc
     1861 gcaatcctcg ccagtgaacc cggacgatat aatcagcttc tccaaaagca agtgagcgga
     1921 tacattcggg acgcgaaccg aatccccggt cagcaatgcg tatctcgtct gccgtttgcg
     1981 caaatcggtc cagccgttca gcgtctctgc tgtcggttag ctcaaaatca gtgaactgac
     2041 aggtatgagg atcatatccc atatgtagtc gccattcagc gctgccgccc ccgggcgcac
     2101 tgattgctgt tccatcgaca agacgcaatc tctttccgct tgtacaaccc gtaactgcgg
     2161 cgcgtacagc aagtgtttgt gcggcaagta tgccaaacca gtcggcggca ttccgcagcc
     2221 gcttcaggag agccacgtca gataatgttg caacgtcatg gagctgagcc catgcagtga
     2281 cttcacgtaa tgacatcccc ccggggccgt aagccagccc cagacgtagc agagttgcag
     2341 catcacgaat ttcgcggcgg cgggttagag ccccggcatt acgtgccgaa gtatccagtt
     2401 cttcgggctt accaatatgg gccagaattg ctgaccagtt atcgtgagag taattcatcg
     2461 gcacgttaaa tcatatcagg cgtaatacca caacccttaa gttagcgctt atggggagaa
     2521 tccctcgcca cctctgatgt gtcaggcatc ctcaacgcac ccgcacttaa cccgcttcgg
     2581 cgggttttgt tttttcctag cattctggtt tacaattcgc acaccagcct gaacaactgg
     2641 cacctgctgc gccagcagag acaaccgatg gcgcacgata ccaaattaca caattctgat
     2701 aattcagccg tctttgccag caggcgcaga cggtgttttc acgcattcaa atctgactgg
     2761 taccagcatc ccccatgcac tgaagaacag gccgaatggc tcattcagtg ttaccgcagg
     2821 cgcggatacg aggttaaaaa agcccttagc ctcgactacc gtcactggat aatctccgtc
     2881 aggcttcctt actctgaacg cccaccgcgt ccgtcccgca cattccagca acgcatctgg
     2941 aggtaacgtg cgggtattac ttcgacctgt tctggtaccg gaactcgggc tggtgatcgt
     3001 taagccgggc cgtgaatcca tgccggtatt ccacaatacc cgggtactgg tggagccgga
     3061 accgaaaagc atgcgtaatc tgccgtccgg ggtcgttcct gccgttcgcc agccgctggc
     3121 ggaggataaa tcattactgc catttttcag cgacgaacga gtgattcgtg ctgctggtgg
     3181 cgctggcgca ttgtctgact ggttactgcg ccatgttaaa tcctgccagt ggccacacgg
     3241 cgattatcac cacagtgaaa ccgtcattca ccgttatggt accggcgcaa tggtgttgtg
     3301 ctggcactgc gacaaccagt tgcgtgacca gacctccgaa tcacttgagc aacttgctca
     3361 ccaaaacctg tcagcatgga tgattgacgt cattcgtcac gcaatgaatg gcacacagga
     3421 gcgtgaatta tcgctggccg aattatcctg gtgggcggcc tgcaatcagg tggtggatgc
     3481 attacctgag gcagtagcgc gtcgttcgct gggattacca gcggaaaaaa tccgctccgt
     3541 ataccgtgag agtgacatcg taccgggaga acagacagcc atcagcatac tgaagcagcg
     3601 cacaaaaaat attgcgctgc cacttcacgt ccaccagcaa caaaatccac cacagaaaaa
     3661 aacggttgtc agtatcgctg ttgatccgga gtctcctgaa tcgttcatga agcggcctaa
     3721 acgtcgccgt tgggttaatg agaaatacac gcgctgggta aagacacagc cgtgtgcgtg
     3781 ttgtggtaag cctgctgacg atccgcatca cctgattggt catggtcagg ggggaatggg
     3841 gacaaaggcc cacgatattt tcacgctacc gttgtgccgg gaacatcaca acgaacttca
     3901 tgcagacccg ctggagtttg agaaaaagta cggctctcag gttgagttaa tttttcgttt
     3961 tcttgatcac gcctttgcaa ccggcgtgct tgggtaaaag aggttactga tgcgtataga
     4021 gtttgttttg ccttatccac cgacggtgaa tacttactgg cgacgtcgtg gcagcacata
     4081 ttttatatct gaggagggaa agcgctatcg ccgggcagtg gcgcttattg ttcgccagca
     4141 gcggctgaaa ttaagcctgt ccggaaggct ggcgataaag gtgattgcag agccaccgga
     4201 taagcgccgc cgtgacctgg ataatatcct gaaagcgccg ctggatgcgc tgacgcatgc
     4261 agggttgtta atggacgatg agcagtttga tgaaatcaat attgtacgtg gccagccagt
     4321 atctggtgga cggctggaga taagaattac agaggtgggt gtgcatgaat aaccagtatt
     4381 tacagtttgt tcgtgagcaa ctcatgattg ccactgcgga tctcagtgtg tcgataaaag
     4441 gccaactgga agcctggcag gaaaatgcac tgttcgatac agggcgttac agacgcaaaa
     4501 aaattcgtta ccgcgatgag gtaaccggaa aaatgatcac gcgggataac ccaccgatcc
     4561 cgggtaaaca atcactggcg aaagggacgt caattgctct ggtcagtccg gttgagtttt
     4621 cgacatcatc atggcgacgt gcagttctgg aactggaagc gcatcagaag gcgtggttgt
     4681 tgtggtgtta cggcggaaac atttgctggg agtatcagat tgcgataacg cagtgggcgt
     4741 ggaatgaatt taatactcaa tccggtacca gaaaaatagc tgggaaaacg ctggaacgtc
     4801 tgaagaagtt gatctggctg gcggcgcaag cggtaaaagc tgagcttttt ggtggggaag
     4861 gttatgaata ccgggatctg gcatcactgg tgggagtgac atcaaaaaac tggtctgaga
     4921 catttactga acgctgggtt gcaatgaagc acatttttct acagcttgat agccaagctt
     4981 tattgctttt aacgaaaaca cgttcaaaac aaaagaccac attttcacag caaaatattg
     5041 caaaactgga ttaaaaagca tatatttcat gtaaatctga tattttgccg attttgtacg
     5101 cgatggcaaa gtaagcaaac cccgccgccg agcgggtttt tttgttggct taacaggtta
     5161 taatgtacag tgcgaaactg cctgttcagc agaatcattt tgtttgtagt ggagttcaca
     5221 agtcgtcatt atcaggcaaa gagatttatg gtgttaaagt atcagccgtc tgttcgttct
     5281 gttttaatgt gtgattttcg gggaatggtt gttccggaaa tagtcaaggt caggccagtg
     5341 gtagtcgtgt ccagaaacag acacaacaat caattggtaa cagtggtacc aataagcact
     5401 actgaaccga ttcctcgcag agattgccat catgagttat cagaaaaccc catccctggt
     5461 aatgagcata ttacttgttg ggtaaaatgt gacatgttga tgacggtttc attgagccga
     5521 ctggatcgca taaaaactag aacctgggag gggcgaaatt atattgttcc tatgattgcg
     5581 gaagatgagt ttgagaatat taaacgggcg gtattgcacg ggatagggat ggcttatctg
     5641 tatcgataat cgaaaacgat tatcgaattc gatatgaatt tatattgaca cacatagtgt
     5701 gttgactgat actgtcgctg taccctgatg ggacttgtga gacttccgaa ctggaagcca
     5761 ggagtagcaa taaggtgatg acaagcctgc tgcccctgtg taaaaggcac cttaatggtg
     5821 cctttgtcgt ttttatcaaa agatccccgc cactggcggg tttttttatg cccgaaaaac
     5881 gacacagggc gttaaacgcg ctggtggttg cgaatacggg tctttcagct tgctggcttt
     5941 ttcgacaaga gttattggta tgtcacgtta acc
//
LOCUS       DAZFRN010000237         5905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFRN010000237 DAZFRN010000000
VERSION     DAZFRN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.239"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            378..941
                     /locus_tag="ACHKUB_004796"
     CDS             378..941
                     /locus_tag="ACHKUB_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6657697.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQSMLAGYYWVMEYTQNKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGDGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            1096..3456
                     /locus_tag="ACHKUB_004797"
     CDS             1096..3456
                     /locus_tag="ACHKUB_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO6657698.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQLGICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTAGLYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRASGGSVFVDMNLQPYPDQWAFLVSVIPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEGKKSSGNRLNLAVAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVTGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIARRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRHGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTASRPKESPHNLEVLTRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDDIASVMNTLKLSPFVLHSRLSKKKRTM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIVADGEGLSF"
     gene            complement(3474..4100)
                     /locus_tag="ACHKUB_004798"
     CDS             complement(3474..4100)
                     /locus_tag="ACHKUB_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657699.1"
                     /translation="MTLRLTLIDELIRKQHHYLEDGDLCYCFGEYTARQGAAYSETNQ
                     LIINLKKGYERRAFPDYHYKKVAIEYVAQMLISNIGNLEEYTFVPVPPSKCRTDAAYD
                     DRMTEILRIAQTYEPWLDYRELVVQNVSTIASHTATNRPSPEAIMANYQLDEQLLEGC
                     RPWIVIFDDVLTAGSHFKAMKSLILQHIPEACILGLFVARTTRGAQII"
     gene            complement(4097..4990)
                     /locus_tag="ACHKUB_004799"
     CDS             complement(4097..4990)
                     /locus_tag="ACHKUB_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO6657700.1"
                     /translation="MSQFDFTSPDPFARAISPFEEMAAYEALWSEKGATFKTIADKFR
                     AAPTGSLPSDFVSDSIIAAFKNRLKEIIARYDIDDLGIRVHGAGEYPEKLRDARNPIE
                     LLYYQGWWDLINTRSVAVVGSRRVSEEGAKRTRKVVKFLVEDGFTIVSGLAEGVDTNA
                     HQTALALGGKTIAVIGTPLSHHYPRQNSNLQKNIRDNHLLISQVPFHRYLEQDYRSNR
                     IFFPERNVTMSALTEATIIIEASETSGTLTQARAALAQGRKLFILESCFRNPSITWPA
                     RFEAQGAIRVRNYEDIRRHLR"
     gene            5585..>5905
                     /locus_tag="ACHKUB_004800"
     CDS             5585..>5905
                     /locus_tag="ACHKUB_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO6657701.1"
                     /translation="MQHRQDNLLANRTLLPGMASGQYVFRLCTFSPVVRYFSLLPCLC
                     ILSFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPEMSAGTEGP
                     CFTVS"
BASE COUNT         1492 a         1291 c         1463 g         1659 t
ORIGIN      
        1 ggctctctct gcagagtctc tgtttttact taatggtacg gcaatactaa tagcgatgtt
       61 gcctgtggct attcgatagc tgacttttgt gaagatcaga ttacttcctg taagtgattg
      121 aatggtactt tcttaaactt aattctatga ggtgtgtttt taccattgta attcgtactg
      181 gtcctcagtt taagagagtc tcactgtatt ctgaagtatt actcccatta ctgtaatgag
      241 gtaatacgaa acagttttat caggttgtct gtgctgctgg ttacctcgtt tattaatgcc
      301 gcggtacggc gtttctgtac tctcagtatt tatgtaattc cactttcctg tcagttttat
      361 aaagtaagga ctataaaatg tataatgcta atcccaatta cgaaatggat ttcatgattc
      421 tgaaagacgt gaatgaacac atggaaggtc tgtttcaacg tttttctaaa ctgttacctt
      481 tcagaattga ttttgcctac aggaaagaca cgcccagttt cgggcattcc tgtaagcaca
      541 gtatgtgtat ggaaatatac cgcttactta gcgaaaccca atcgatgttg gcgggatatt
      601 actgggtaat ggaatataca caaaacaagg gacttcatat tcattttatc ggttacctgg
      661 atgggcaacg tcacaagaac tcttatcaga tatccaggca actgggagat atctggaggc
      721 ggatcacgga aggggatggt tattttcacc tgtgccgggc taaagataaa tacccggtgc
      781 gtatcgatca cgtgattcac tatagtgata aatctgcggt agacgactta cgttacgccc
      841 ttagctatct ggcgaaacag gatcagaaag aacacggtat tattctgggc cgtagcaggc
      901 ttcctgaaaa aagtaaccgc ggccgaccac gacacaactg aattacagct gtattaggta
      961 ttagaattaa gtttctgtaa gttgttggga ggtgcttgag ttaataggca gaagagggcg
     1021 gggcgctttg tcatcagtgt aggggacccg aatttctgcc gtggtgcctc agtggatcgg
     1081 atacaggtaa cgaatatggt tcataaatct gacagtgatg aattagctgc attgagggca
     1141 gaaaatgccc gtctggtctc attacttgaa gctcatggga ttgaatggcg acgtaaaccg
     1201 cagactcctg tgcagcgcgt ttctgtatta tccaccgacg agaaggttgc attatttcgt
     1261 cggttgtttc gtgggcgtga tgatgtatgg gcacttagat gggaaagtaa aaccagcggc
     1321 aaatcagggt actcgccggc ctgcgctaac gaatggcagt tgggaatatg cggtaagccc
     1381 cggataaaat gtggggactg tgctcaccgt cagttgattc ctgtatccga tctcgtcatc
     1441 taccatcatc tggccggtac ccatactgcc gggttatatc cgttgctgga agatgattcc
     1501 tgttattttc tggcagttga ttttgatgaa gctgaatggc agaaggatgc atccgcattc
     1561 atgcgatcct gtgatgagct gggtgtacct gctgcgctgg aaatatcccg ttcacgtcag
     1621 ggggcgcatg tctggatatt ttttgcctca cgagtttcgg cccgcgaagc tcgccgtctg
     1681 gggactgcta ttattagcta tacgtgtagt cggacccgac agctgcgatt ggggtcttat
     1741 gaccgattat ttcctaatca ggatactatg ccaaaagggg gatttggtaa tctcatagcg
     1801 ttacctctgc aaaaaagacc gcgtgcatcg gggggaagcg tttttgttga tatgaatctc
     1861 cagccttatc ctgatcagtg ggcttttctt gtatcggtga tcccgatgaa tgtgcaggat
     1921 attgaaccga cgatattacg ggctacaggg agtatccatc ctctggatgt gaattttatc
     1981 aacgaagaag acctgggtac gccgtgggaa gggaaaaaat catcaggaaa cagactgaat
     2041 cttgctgttg ctgaaccgct gaaaatcacg ctggcaaatc agatctattt cgaaaaagcg
     2101 caattacctc aggtgctgat taaccgactt attcggctgg cagcatttcc gaaccctgag
     2161 ttttataagg ctcaggcaat gcgtatgtca gtctggaata agccccgtgt tacaggttgt
     2221 gcggagaatt acccgcaaca cattgcgttg ccccggggat gtctggacag cgtattatct
     2281 ttccttaggg acaacaatat tgctgcagaa ttaatcgata aacgatttgc cgggacggaa
     2341 tgtaatgccg tttttatggg aaacctcaga gcggagcagg aagaggccgt ttcggcatta
     2401 ctccgttatg acactggtgt gctttgtgcg ccaacggctt ttggtaagac agttaccgca
     2461 gcggcagtga ttgccagacg gaaagtgaat acactgatac tggtacaccg gactgaattg
     2521 ctgaagcagt ggcaggagcg tctcgcggtg tttctgcagg ccggtgacag tattggtatt
     2581 atcgggggag gtaaacataa accctgtggc aatattgata ttgcggtggt gcagtccata
     2641 tccagacacg gagaagttga acctctggtc aggaattatg ggcaaatcat tgtggatgag
     2701 tgccatcata ttggcgcggt ttcattttct gcgattctga aggaaacgaa tgctagatat
     2761 ctgcttggcc tgacggcaac accaatccga cgggatggtc tgcaccccat tatttttatg
     2821 tactgtggtg ccattcgcca tacagcgtcc cgcccgaagg aaagcccaca taatctggag
     2881 gtgctgaccc gttcccgttt tacatctggt catttaccat cggatgcgag aatccaggat
     2941 attttcagag aaattgctct ggatcatgac agaacggtgg cgatagctga agaagccatg
     3001 aaagctttcg ggcaggggcg taaagttctg gtactgactg aacgtacaga tcatctggat
     3061 gatatagcct cagtgatgaa cacactgaaa ttgtcgcctt ttgttctcca tagtcgatta
     3121 tcgaaaaaaa agcgtactat gctgatttcc gggctgaatg ctcttcctcc tgattctccc
     3181 cgtattttgt tgtcaacagg cagacttatt ggtgagggat ttgaccaccc tccgctggat
     3241 acgctgattc ttgccatgcc tgtgtcatgg aaagggacgt tacagcagta tgcgggacgt
     3301 ctacacagag agcataccgg taaaagcgat gtcaggatca ttgattttgt ggataccgcg
     3361 tatccagtgc tgctcagaat gtgggataaa cgccagcggg gttataaggc gatggggtac
     3421 aggattgtgg ctgatggtga agggctttca ttctaaggag tactttaccc tttttaaata
     3481 atttgagctc cccgggttgt tcgggcaaca aagagtccca gtatacaggc ttcaggaata
     3541 tgttgtagta tcaggctttt catcgcttta aaatggctac cggcagtaag aacgtcatca
     3601 aaaataacaa tccagggcct gcatccctcc agtaattgtt catccagttg gtagtttgcc
     3661 ataatcgctt ctggtgaggg ccgatttgtt gctgtatgag aggcaattgt tgatacgttt
     3721 tgcacaacca gttcccggta atccagccag ggttcgtagg tttgcgcaat tcgcagaatt
     3781 tctgtcatac ggtcatcgta agcggcatct gtacggcatt tggatggtgg tacgggaaca
     3841 aatgtgtatt cctcaagatt accgatattt gatatgagca tctgagcaac atactcaata
     3901 gcaacctttt tatagtggta atcagggaat gcccgacgtt catacccctt tttaagattg
     3961 atgattaact ggttagtttc gctgtaagca gcgccttgtc gggctgtgta ttcgccaaag
     4021 caataacaca gatcaccatc ctccagataa tgatgttgtt tacgtatcag ttcatcaatc
     4081 agagtcagac gaagtgtcaa cggagatgcc tcctgatgtc ttcgtaattc ctgacccgaa
     4141 tggccccttg cgcttcaaaa cgggcaggcc atgtaattga tgggttacga aagcaggatt
     4201 ccagaataaa caattttcgg ccctgggcaa gtgccgcacg tgcctgagtc agagtacctg
     4261 atgtttctga tgcttcaata ataatcgttg cttctgtcag cgcagacatc gtgacatttc
     4321 gctccggaaa aaatattcgg ttgctccgat agtcctgctc caggtatcga tgaaacggaa
     4381 cctgactgat aagcaggtgg ttgtcgcgaa tgtttttctg cagattactg ttttgtctgg
     4441 gataatgatg tgaaagtggt gtcccgataa ctgcaatcgt tttaccaccc agagccagtg
     4501 ccgtctgatg tgcgttggta tccacgcctt cagccagccc tgaaacgatg gtaaaaccat
     4561 cctcaaccag aaatttgacg actttgcggg ttcgttttgc tccttcttca gagactctac
     4621 gggaaccaac aacagctacc gagcgagtgt taatcaggtc ccaccacccc tgatagtaaa
     4681 gaagttctat gggattccgc gcatcccgca gtttttctgg atattctccg gcaccatgga
     4741 cacgtatacc cagatcgtca atatcgtaac gtgcgataat ttctttcagc cggtttttaa
     4801 aagcagcaat aatgctgtct gagacaaagt cagaaggaag cgagccggtt ggtgctgcac
     4861 gaaatttatc cgcaatagtt ttgaatgtcg cgcctttttc tgaccataac gcctcataag
     4921 ccgccatttc ctcaaaagga gaaatggctc gggcaaaggg atccggactg gtaaaatcga
     4981 actgcgacat aagcattcag ggataataaa caggatgaac gggtagttta cctgttcagg
     5041 ctccggtgta aaccccggag gtggatgctg ctgataatgc cacaatcgac agaaactgtg
     5101 aaattgtcgt aatttttatt cagactgaat tttattgcaa tgcctggtca gggatggtgg
     5161 gttacatcaa agatgagaaa cgtgcaaaat gccccctgaa tcgatcacag ttcggcatac
     5221 gcaaaacgcg ttatcagtag tgactggaat catcgttatt gcgcattttc catttataag
     5281 aacacactga ggagtttcag gtcctcagga gagttatgac tgttttttta ctcaaatcca
     5341 caacacctga actggaactt gtgttgcacc cctgattgtt actctgcagg aaacatcttt
     5401 tttatcatca aaggatgact gttttccttt ctcccctccg taaaacacaa ctccggtcac
     5461 atttctgata tttttccagg atcttacata gcaggactgt ttctgtatgt tttttatctg
     5521 gtgtaaattt cagcactgac actccgctta cgttaattta cactggatac cccacgagga
     5581 gaatatgcag caccggcagg ataacttact ggcaaacaga acattgttgc ccggtatggc
     5641 ttccggtcag tacgtattca ggctctgcac tttctctccg gtggtacgct atttttccct
     5701 cctcccctgc ctttgcattc tttcgttttc gtctccggca gccatgctgt ctccgggtga
     5761 ccgcagtgca attcagcagc aacagcagca gttgctggat gaaaaccagc gtcagcgtga
     5821 tgcgctggag cgcagtgcgc cgctgaccat cacgccgtct ccggaaatgt ctgccggtac
     5881 tgaaggtccc tgctttacgg tgtca
//
LOCUS       DAZFRN010000238         5846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFRN010000238 DAZFRN010000000
VERSION     DAZFRN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.240"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..267
                     /locus_tag="ACHKUB_004801"
     CDS             <1..267
                     /locus_tag="ACHKUB_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO6657702.1"
                     /translation="QRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILS
                     KMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            630..1667
                     /gene="dusA"
                     /locus_tag="ACHKUB_004802"
     CDS             630..1667
                     /gene="dusA"
                     /locus_tag="ACHKUB_004802"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO6657703.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1801..2043
                     /gene="pspG"
                     /locus_tag="ACHKUB_004803"
     CDS             1801..2043
                     /gene="pspG"
                     /locus_tag="ACHKUB_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO6657704.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2209..3192)
                     /locus_tag="ACHKUB_004804"
     CDS             complement(2209..3192)
                     /locus_tag="ACHKUB_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO6657705.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAATGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3275..4690
                     /gene="dnaB"
                     /locus_tag="ACHKUB_004805"
     CDS             3275..4690
                     /gene="dnaB"
                     /locus_tag="ACHKUB_004805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6657706.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4743..5822
                     /gene="alr"
                     /locus_tag="ACHKUB_004806"
     CDS             4743..5822
                     /gene="alr"
                     /locus_tag="ACHKUB_004806"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO6657707.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
BASE COUNT         1412 a         1413 c         1553 g         1468 t
ORIGIN      
        1 caacgtgata tgaagaagta cctgggagag atgaagcgtc tggctgatgg gggagaaatg
       61 aattttgatg gctatctgga taagatgtca catctggtga atgaaggaac gctcgatcct
      121 gatattttaa gcaaaatgcg agatgctgca ccacaattaa ttagcttcgc gaagtcgttt
      181 gacccaacct caaaggaaga gattaaaata cttacagaca cttctaaatt aatttatgat
      241 ttgttcgggg ttaaatcgga gaaataatat gtgaagttct tcgatagtat ggaaggcatt
      301 atataaaagg acccaatatt tattgggttc ttttttctct atcaatgcta ttagcaggga
      361 gatatatcac cagagtttaa tgtgtgattt tttatttatc gtcgaacctg gattgtttat
      421 cattggcctt aacaaagtta acggctaata agattatttc catcacttcg tgagagcttc
      481 atgccttgag aggatctcaa ttttcttttg caatgagaca ggcgcttcct gttgttatgg
      541 tatagtaccc cgctattgag cctcctgaat agtgatgctg aataacataa ccccatgata
      601 tatcgataaa ataatctcta catttgaaaa tgcacggtaa ttctgaaatg caaaaaatca
      661 accaaaccag cgcaatgcct gaaaaaactg acgttcactg gagtggtcgg tttagcgttg
      721 caccaatgct cgactggacg gacagacatt gccgctattt cttgcgtctg ctttcccgca
      781 atacgttgct gtataccgaa atggtgacca caggggcgat tattcacggt aaaggtgatt
      841 acctggcgta cagtgaagaa gaacatccgg tagcgttgca actcggcggt agcgatccgg
      901 cggcgctggc acagtgtgcg aagctggcag aagcgcgtgg atatgatgag atcaacctga
      961 atgtcggctg cccgtctgac cgggtgcaga acggcatgtt tggtgcgtgt ctgatgggta
     1021 atgcgcagct ggttgccgac tgcgtgaaag cgatgcgcga tgtggtgtcg attccagtga
     1081 cggtgaaaac gcgtattggc atcgatgacc aggacagcta tgaatttctc tgcgatttca
     1141 tcaacaccgt ttccggcaaa ggcgagtgtg agatgtttat catccacgca cgtaaagcct
     1201 ggctttcggg gttaagcccg aaagaaaacc gtgaaatccc gccgctcgat tatccgcgtg
     1261 tgtatcaact gaagcgtgac tttccgcatc tgacaatgtc gattaacggt ggtatcaagt
     1321 cgctggaaga ggccaaagca cacctgcaac atatggatgg cgtgatggtc gggcgcgagg
     1381 cgtatcagaa tccgggtatt ctggcggcgg tagaccggga gatctttggt tcctcggata
     1441 ccgatgccga tccagtggcg gtagtgcgcg ccatgtatcc gtacattgag cgtgaactca
     1501 gccaggggac gtatctcggc catattaccc ggcatatgtt gggcttgttc cagggtattc
     1561 ctggcgcgcg gcagtggcgg cgttatttaa gtgaaaatgc ccataaagcg ggtgcagaca
     1621 ttaatgtgct ggaacacgcg ctcaaactgg tggcggataa gcgttaactt ttcaccaaaa
     1681 agtagtcaaa ttcaccacgc cctgcgcacc gtcgcggggc attttgctgt taaatcaata
     1741 gattattttt ggcatgattc ttgtaatgcc agcaagagat ttcatatttg ggagagcatc
     1801 atgctggaac tactttttgt gtttggcttt tttgtcatgc tgatggtcac cggcgtttcg
     1861 ttgctgggca ttatcgccgc gctggttgtg gcgacggcca ttatgttcct cggcggtatg
     1921 ctggcattga tgattaagtt gctgccgtgg ttactactgg cgattgcggt ggtgtgggtt
     1981 attaaggcga ttaaagcacc aaaagtgccg aaatatcagc gttatgaccg ctggcgttac
     2041 taagggattg tgcggatgat cacaacctaa ggttttatcc ttagaacaaa ataggaattg
     2101 ataatcaaat ctgtcactat tgcgcctcta acagattcat cgtgctgtac cctacataca
     2161 gccgaactat aaaaagaaag ggcttcccag gtggaagccc tatttctttt atggaatcag
     2221 caggctggaa ccttgcgtcg cccggctttc cagaatctca tgcgcacgct gcgcatcctt
     2281 cagcggatat ttctgctgct cggcgacatc gaccttaatc acaccgctgg caatcaaaga
     2341 gaacagttca ttactggcct cggttaattc ctcccgcgtg gtgatatagc cttgcaggga
     2401 agggcgtgtc acatacaacg agcctttttg attgagaatg cctaagttca caccggtaac
     2461 cgcacctgat gagttgccaa aactgaccat taagccgcgg cgttgcaggc aatccagcga
     2521 ccgttcccag gtgtctctgc ccacggaatc gtacactacg cgcactttct taccgccggt
     2581 gatctctttc aatcgctcga ccagattctc ttcacgatag ttaataacct gccacgcgcc
     2641 ggcttttagc gcgctctgcg ctttttgcgc ggttcctacg gtgccgataa gtttcgcgcc
     2701 cagggctttt gcccactggc aggcaattaa gccaacgccg ccagtcgctg cgtggaacag
     2761 gaactgttca tcgggtttaa tttcataggt tttgcgcagc agataataaa ccgttaagcc
     2821 tttcaggaaa gatgctgcag cttgttcaaa agaaattgcc gcaggcagaa tcgccgcttt
     2881 atccgcatta atgttatgca cagagctgta agcgcctaac gccgactgcg catagactac
     2941 acgatcgcct gccttaatat gctttacacc actgccgact ttactcacga tgcctgccgc
     3001 ttcggtacct aatccgctgg gtagtgatgg cggcgggtaa aggccgctgc ggatatacgt
     3061 gtcgataaaa ttgatgccga tggctttatt ttcgacctgg atttcattct ctgccggatc
     3121 ggcaggagtg aactctacgg cttgaagtac ttccgggcca ccgtgcttgt gaaattcaat
     3181 tcgtgttgcc atgtgtcctc cagaacgtat cgtcagggtc tgcttcatat ggtaaagttt
     3241 cgacccattc tttatctcgg taactccatt cactatggca ggaaataaac ccttcaacaa
     3301 acagcaggct gaaccccgcg aacgcgatcc acaagttgcc gggctgaaag tgcctccgca
     3361 ctcgatcgaa gcggagcagt cggtgttggg cggtttaatg ctggataacg aacgctggga
     3421 cgatgtagcc gagcgtgtgg tggcagacga tttttacacc cgcccacacc gccatatctt
     3481 tactgaaatg gcgcgtttgc aggaaagcgg tagtcctatc gatctgatta cccttgcgga
     3541 atcgctggaa cgccaggggc aactcgatag cgtcggtggt tttgcttatc tggcagagct
     3601 gtcaaaaaat acgccaagtg cggcgaacat cagtgcctat gcggacatcg tgcgtgaacg
     3661 tgccgttgtc cgtgagatga tttcggttgc gaatgagatt gctgaagccg gttttgatcc
     3721 gcaggggcgc accagcgaag atctgctgga ccttgctgaa tcccgcgtct ttaaaattgc
     3781 tgaaagtcgt gcaaacaaag acgaagggcc gaagaacatc gccgatgtgc tcgacgcaac
     3841 cgtggcgcgt attgagcagt tgtttcagca gccacacgat ggcgttaccg gagtaaacac
     3901 cggttatgac gatctcaaca aaaaaaccgc tggcttgcag ccgtcggatt tgatcatcgt
     3961 cgccgcgcgt ccgtcgatgg gtaaaacaac atttgcgatg aacctcgtcg aaaacgcggc
     4021 gatgttgcag gataaaccag tacttatctt ctcgctggag atgccttcag aacagattat
     4081 gatgcgttct ctggcgtcgc tgtcgcgcgt tgaccagact aaaatccgta ccgggcagct
     4141 cgatgatgaa gactgggcgc gcatttccgg caccatgggt attttgctcg aaaaacgcaa
     4201 tatctatatc gatgactcct ccggcctgac gccaacggaa gtgcgttccc gcgcacgccg
     4261 tattgcccgt gaacacggcg gcatcgggct tatcatgatc gactacctgc aactgatgcg
     4321 cgtaccggcg ctttccgata accgtacgct ggaaattgca gaaatctctc gctcgctgaa
     4381 agcactggcg aaagaactga acgtgccggt ggtggcgctg tcccagttga accgttctct
     4441 ggaacaacgt gccgacaaac gcccggtcaa ctccgacctg cgtgaatctg gctctatcga
     4501 gcaggatgcg gacttgatca tgtttatcta tcgtgatgag gtgtatcacg aaaacagtga
     4561 tttaaaaggc atcgcggaaa ttattatcgg taaacaacgt aacggcccaa tcgggacggt
     4621 acgcctgacc tttaacggtc aatggtcgcg cttcgacaac tatgcggggc cgcagtacga
     4681 cgacgaataa taattatttt atgaattagg taattaaagc aaacacttat caaggaacac
     4741 aaatgcaagc ggcaactgtt gtgattaacc gccgcgctct gcgacacaac ctgcaacgtc
     4801 ttcgtgaact ggcccctgcc agtaaaatgg ttgcggtggt gaaagcgaac gcttatggtc
     4861 acggtcttct tgagaccgcg cgaacgctcc ccgatgctga cgcctttggc gtagcccgtc
     4921 tcgaagaagc tctgcgactg cgtgcggggg gaatcaccaa acctgtactg ttactcgaag
     4981 gcttttttga tgccagagat ctgccgacga tttctgcgca acattttcat accgccgtgc
     5041 ataacgaaga acagctggct gcgctggaag aggctagcct ggacgagccg gttaccgtct
     5101 ggatgaaact cgataccggt atgcaccgtc tgggcgtaag gccggaacag gctgaggcgt
     5161 tttatcatcg cctgacccag tgcaaaaacg ttcgtcagcc ggtgaatatc gtcagccatt
     5221 ttgcgcgcgc ggatgaacca aaatgtggcg caaccgagaa acaactcgct atctttaata
     5281 ccttttgcga aggcaaacct ggtcaacgtt ccattgccgc atcgggtggc attctgctgt
     5341 ggccacagtc gcattttgac tgggtgcgcc cgggcatcat tctttatggc gtctcgccgc
     5401 tggaagatcg ctccatcggt gccgattttg gctgtcagcc agtgatgtca ctaacctcca
     5461 gcctgattgc cgtgcgtgag cataaagccg gagaacctgt tggttatggt ggaacctggg
     5521 taagcgaacg tgatacccgt cttggcgtag tcgcgatggg ctatggcgat ggttatccgc
     5581 gcgccgcgcc gtccggtacg ccagtgctgg tgaacggtcg cgaagtaccg attgtcgggc
     5641 gcgtggcgat ggatatgatc tgcgtagact taggtccaca agcgcaggac aaagccgggg
     5701 atccggtcat tttatggggc gaaggtttgc ccgtagaacg tatcgctgaa atgacgaaag
     5761 taagcgctta cgaacttatt acgcgcctga cttcaagggt cgcgatgaaa tacgtggatt
     5821 aatcgttctg taatatttga ttgtct
//
LOCUS       DAZFRN010000239         5832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFRN010000239 DAZFRN010000000
VERSION     DAZFRN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.241"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            265..975
                     /locus_tag="ACHKUB_004807"
     CDS             265..975
                     /locus_tag="ACHKUB_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6657708.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1017..3668
                     /locus_tag="ACHKUB_004808"
     CDS             1017..3668
                     /locus_tag="ACHKUB_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6657709.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSELLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPELWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSNSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3682..4212
                     /locus_tag="ACHKUB_004809"
     CDS             3682..4212
                     /locus_tag="ACHKUB_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6657710.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4225..4728
                     /locus_tag="ACHKUB_004810"
     CDS             4225..4728
                     /locus_tag="ACHKUB_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6657711.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4787..5701
                     /locus_tag="ACHKUB_004811"
     CDS             4787..5701
                     /locus_tag="ACHKUB_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6657712.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
BASE COUNT         1696 a         1252 c         1285 g         1599 t
ORIGIN      
        1 ttttaatgag aaatggtttg tatttggagc gctatgaaaa atcacataat atctaaacta
       61 tcaataaaca acagtagtta aacttactgt tatttaaaat atttactttg tagcaaggtt
      121 ttcctattaa aagagaacca acatttatta caaacgcatt aagtctggaa acccggcgac
      181 tcgccaatgt attagcaaca ttaatcgatt gtctgatgta acggttttcc cgcttatcct
      241 cttaacacat ggagaatgca tcagatgcaa actacgcgta ccccctactc tatttccttt
      301 atggcgacag tgttactttt attgctcttt gcttgccata gcaccgttgc caacgcagct
      361 gtcgcactgg gtgcgacgcg ggtaatttat ccggcaaacc agaaacaggt tttattgcct
      421 gtcacgaata atgatcctgc cagtgtctat ctcattcagt catggataga aaatgcaggt
      481 gaccaaaaag acacccagtt tgtcattacc ccaccgcttt tttccatgca aggtaaaaaa
      541 gagaatacgc tgcggataat caacgctact aatcatcagt taccaggcga ccgtgaaagc
      601 ctgttctggg tgaatgttaa agctattcct gctatggaaa aagaccagaa gaatgagaat
      661 acattacagc tggcaattat tagccgcata aaaatgtttt atcgccccac gaacctggca
      721 atggcacctg aagaagcgcc tgcaatgctt cggtttcgtc gcagcggtag caaactcacg
      781 cttattaatc caacgcccta tttcattacc gtaacaaata tgaaagcagg aaatagcaat
      841 ctcccaaaca ccatggtgcc gccaaaaggt gaagtatcgg tggatatcac gcatgctgca
      901 accggggata tcagttttca gacgatcaat gattacggtg cattaactcc gcgtattaag
      961 gcaaccatgc agtaagccag tgacctaatt aataataaac tcaatcggaa ttaataatga
     1021 cagccattcg tgccgcattt aaggcatatc gtatgcatca ggtgttaata atgcctcgct
     1081 ttgcacggct taccattgcc cttggcttag caactgcggt atttcccgtt gacgcagagt
     1141 attattttaa tccacggttt ttaagcaatg atcttgctga atctgtcgat ttatccgcct
     1201 ttactaaggg acgcgaggca ccacctggta catatcgggt cgacatttat ttgaacgatg
     1261 aatttatgac tagtcgggat attactttta ttgcagatga taataatgca gaactcatcc
     1321 catgcctgag tacagatctt cttgtcagtc ttggtataaa aaaatcagag ttattagaca
     1381 ataaagaaca ttcggcagaa aaacatgtgc cggacaacag cgcatgcaca ccacttcagg
     1441 atcgtttggc cgacgcatca actgagtttg atgttggtca gcaacatttg tcactctccg
     1501 ttccgcaaat ttatgttggc agaatggctc gcggctatgt ttctcctgaa ctgtgggaag
     1561 aaggaataaa tgctgggctt ctaaattata gttttaatgg taattccatt aataatcgta
     1621 gtaaccataa tgcaggaaaa tccaactatg catatttgaa tttacagagt ggcatcaaca
     1681 ttggtagttg gcgactgcgc gataactcaa cgtggagtta taacagtggg agtagcaatt
     1741 catctaacag caataaatgg cagcatatca atacgtcggc tgaacgtgac attattccct
     1801 tacgctcacg gttaacggta ggtgatagtt ataccgatgg cgatattttt gatagtgtga
     1861 actttcgtgg cctcaaaata aattcaacag aagcgatgtt gcccgatagc caacatggtt
     1921 tcgctccggt gattcatggt attgcccgtg gcaccgcaca agtgagtgta aaacagaacg
     1981 gatacgatgt ttatcagact actgttccac ccggcccttt tactattgat gatatcaact
     2041 ctgccgccaa tggtggtgat ctgcaagtaa ccataaaaga ggcagacggc agtattcaga
     2101 cattatatgt tccttattcg tctgttccgg ttctccaacg tgctggatat acgcgttatg
     2161 cgcttgccat gggggaatat cgtagtggaa ataacctgca aagctccccc aagttcatac
     2221 aaggtagctt gatgcatgga ctggaaggaa actggacacc ttatggcgga atgcaaattg
     2281 cagaagatta tcaggccttc aaccttggta ttggtaaaga tttaggactt tttggtgcct
     2341 tttctttcga tatcacgcag gccaatacga cacttgcaga tggcacccgt cacagcgggc
     2401 aatcggttaa atccgtctac agcaaatcct tctaccagac aggaaccaat atccaggtcg
     2461 caggatatcg ctattctacg caaggttttt ataacttatc cgacagtgcc tacagtcgaa
     2521 tgagtggtta caccgtcaag cctcctaccg gagacaccaa tgagcagaca caatttattg
     2581 attattttaa tctgttctac agtaagcgtg gtcaggaaca aataagcatc tctcagcagc
     2641 ttggaaatta cggtacgaca tttttcagtg ccagtcgcca aagttactgg aacacgtcac
     2701 gcagcgacca gcaaatatca tttggattaa atgtgccgtt tggtgatatt acgacttcgc
     2761 tgaattacag ctattccaat aatatatggc aaaacgatcg ggatcattta ctcgctttta
     2821 cgcttaatgt tcccttcagt cattggatgc gtacagacag tcagtcggca tttcgtaatt
     2881 caaacgccag ttacagtatg tcaaacgatt tgaaaggcgg catgaccaat ctatcggggg
     2941 tttatggcac tctgctgccg gataataacc tgaattatag cgttcaggtc ggtaacaccc
     3001 acggaggtaa tacatcgtct ggcaccagtg gttacagttc tcttaattat cgtggagctt
     3061 acggtaatac gaatgtcggt tacagtcgga atggtgacag cagccagatt tattacggaa
     3121 tgagtggtgg gattattgct catgctgatg gcatcacctt tggacagccg ctgggcgaca
     3181 caatggttct ggttaaggct cctggcgctg ataatgtcaa aatagagaac cagaccggaa
     3241 ttcataccga ctggcgtggc tatgcaatat taccatttgc gacagaatat agagaaaatc
     3301 gtgtcgctct taacgcgaat tcccttgcag ataatgttga actggatgaa accgtagtca
     3361 ctgtcatccc aactcacggt gctattgcca gagcaacatt taatgcacaa atcggcggga
     3421 aagtattaat gacgttgaag tacggtaata agagcgttcc attcggtgca attgtcacac
     3481 acggagagaa taaaaatggc agcattgtcg cagaaaacgg tcaggtttat ctgactggac
     3541 ttccacagtc agggaaatta caggtttcat ggggcaaaga taaaaactca aactgtattg
     3601 tcgagtacaa gcttcctgaa gtttctcctg gtaccttact gaaccagcag acagcaatct
     3661 gtcgctaagg aacaatagat tatgaaatac aataacatta ttttcctcgg tttatgtctg
     3721 gggttaacca cctattctgc tttatccgca gatagcgtta ttaaaattag cgggcgcgtc
     3781 ctcgattatg gctgcacagt ctcatcggat tcgcttaatt ttaccgtaga tctccaaaaa
     3841 aacagtgcca gacaatttcc aacgaccggt agcacaagtc cagtcgtccc ttttcagatt
     3901 acgttaagtg aatgcagcaa agggacaacg ggggttcggg ttgcatttaa cggtattgag
     3961 gatgcagaaa ataatacttt gttgaaactg gatgaaggaa gcaatacggc ctccggtttg
     4021 ggtatagaaa tattggacgc aaatatgcgt ccggtgaaac tgaatgacct tcatgccggg
     4081 atgcagtgga tcccactggt accagaacag aacaatattt tgccttactc cgctcgtctg
     4141 aagtcaactc agaagtccgt caatccggga ctggtgaggg cttcggcaac ctttaccctt
     4201 gaatttcaat aatcagagtc agatatgaaa agacttcaca agaggttcct gttagctacg
     4261 ttttgcgcgt tattcacagc aactctccag gccgccgatg tcactatcac tgttaatggt
     4321 cgggtagtcg ctaaaccctg cactattcaa accaaagaag ctaacgttaa tctcggggat
     4381 ctttatacgc gcaatctgca acaacctggt tctgcatctg gctggcacaa tattactttg
     4441 tcattaaccg attgtccggt tgaaacaagt gcagtgacgg caatcgtgac aggttcaact
     4501 gacaatacgg gttattacaa aaatgaaggt actgccgaaa atattcagat agagctgagg
     4561 gatgaccagg atgctgcgtt aaaaaatggc gatagcaaaa cggttattgt tgatgagatc
     4621 actcgtaatg cacagtttcc acttaaggca agagctatca cggtgaatgg aaacgcaagc
     4681 cagggaacga tcgaggcgct aatcaatgtg atctacacct ggcaataacg tcgattcgat
     4741 tttcttagcc ttctttttaa atcaattttt aaccttagga tatatcatgg gtaaaacaat
     4801 cagtataaag gtcctgttcg gtatttatct tctattaatg gcaggcaaag tttttgcctt
     4861 cagttgtaat gtcgatggcg ggtcaagtat tggagcaggt acaacctctg tttatgttaa
     4921 tctcgaccct gtaatacagc cgggccagaa tctggttgta gacttgtctc agcatataag
     4981 ttgctggaat gattacggcg gctggtacga cactgatcat ataaacctgg tacaaggctc
     5041 agctttcgca ggctccctac agtcatataa aggatcgctg tactggaata atgtgaccta
     5101 ccccttcccg ttgacgacaa ataccaacgt attggatatt ggtgataaga caccgatgcc
     5161 attaccgtta aaactctata ttaccccagt tggggcggcc ggtggagttg tcataaaagc
     5221 gggggaagtc attgctcgta ttcatatgta taaaattgca acattaggaa gcggaaatcc
     5281 gcgtaatttt acgtggaata ttatttcaaa taacaatgtt gttatgccaa caggcggctg
     5341 caccgttgat agtcgtaatg tcactgtgga tctcccggac tttcctggaa gcgcagaaat
     5401 accccttggc gtatattgct caagcgaaca aaagctctca ttttatcttt cgggtgccac
     5461 cactgacagt agccgacaag tgtttgctaa cacagcacct gatgcaacaa aagcatcggg
     5521 agtgggtgtc tctctgatgc gtaatggaaa aatactggct acgggagaaa atgtctccct
     5581 ggggacagta aataaaagca aagtacctct gggtttaagc gcaacctatg gacaaacagg
     5641 aaacaaggtt gcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata
     5701 attgtgattt tgatcttagt ctatgcgctc cattcacgaa aattgcgcgg ataattattt
     5761 tgtgaaggct attatcctac acctgtaaga aaatccgcgc acaattgaag ccagagtctt
     5821 attttttata tt
//
LOCUS       DAZFRN010000240         5806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFRN010000240 DAZFRN010000000
VERSION     DAZFRN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.242"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>5806
                     /locus_tag="ACHKUB_004812"
     CDS             <1..>5806
                     /locus_tag="ACHKUB_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO6657713.1"
                     /translation="TGRLYSAGNLLLDAQDFSGQGQVVATGDVTLKLIAALTNHGTLA
                     AGKTLSVTSQNAITNGGVMQGDAMVLGAGEAFTNNGTLTAGKGNSVFSAQRLFLNAPG
                     SLQGGGDVSLNSRSDITISGFTGTAGSLTMNVAGTLLNSALIYAGNNLKLFTDRLHNQ
                     HGDILAGNSLWVQKDASGGANTEIINNSGNIETHQGDIVVRTGHLLNQREGFSATTTT
                     RTNPSSIQGMGNALVDIPLSLLPDGSYGYFTREVENQHGTPCNGHGACNITMDTLYYY
                     APFADSATQRFLSSQNITTVTGADNPAGRIASGRNLSAEAERLENRASFILANGDIAL
                     SGRELSNQSWQTGTENEYLVYRYDPKTFYGSYATGSLDKLPLLSPEFENNTIRFSLDG
                     REKDYTPGKTYYSVIQAGGDVKTRFTSSINNGTTTAHAGSVSPVVSAPVLNTLSQQTG
                     GDSLTQTALQQYEPVVVGSPQWHDELAGALKNIAGGSPLTGQTGISDDWPLPSGNNGY
                     LVPSTDPDSPYLITVNPKLDGLGQVDSHLFAGLYELLGAKPGQAPRETAPSYTDEKQF
                     LGSSYFLDRLGLKPEKDYRFLGDAVFDTRYVSNAVLSRTGSRYLNGLGSDTEQMRYLM
                     DNAARQQKGLGLEFGVALTAEQIAQLDGSILWWESVTINGQTVMVPKLYLSPEDITLH
                     NGSVISGNNVQLAGGNITNSGGSINAQNDLSLDSSGYIDNLNAGLISAGGSLDLSAIG
                     DISNISSVISGKTVQLESVSGNISNITRRQQWNAGSDSQYGGVHLSGTDTGPVATIKG
                     TDSLSLDAGKNIDITGATVSSGGDLGMSAGNDINIAANLISGSKSQSGFWHTDDNSSS
                     STTSQGSSISAGGNLAMAAGHNLDVTASSVSAGHSALLSAGNDLSLNAVRESKNSRNG
                     RSESHESHAAVSTVTAGDNFLLVAGRDIASQAAGMAAENNVVIRGGRDVNLVAESAGA
                     GDSYTSKKKKEINETVRQQGTEIASGGDTTVNAGRDITAVASSVTATGNISVNAGRDV
                     ALTTATESDYHYLETKKKSGGFLSKKTTRTISEDSATREAGSLLSGNRVTVNAGDNLT
                     VEGSDVVADRDVSLAAGNHVDVLAATSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTT
                     HDRREAGTTQSQSASTIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGH
                     DRRTVDEKFEQKKSGLTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSG
                     VQAGQAATMASATGDPNAMGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATG
                     KNRGDNRGDIVIAGSQLKAGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIG
                     AGGNGAGISVFAGVNAAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNR
                     IIADVGHDLLISSQQDTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSV
                     AEQTGMFAGDGGFDITVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVS
                     HSGISLSGGGSFGDKFQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQN
                     LANLSRDPAHANDSISPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAK
                     EATGETLPANATEKQRQEYLAKLRDTQAYRNEMAKYGTGSEIQRGIQAATAALQGLAG
                     GNLAGALAGASAPELAHLLKSTEKDPAVNAIAHAILGGAVAAMQGNNVAAGAAGAATG
                     ELAARAITGMLYPGVKQSDLSEEQKQTISTLATVSAGLACGLTGNSTASAAVGAQSGK
                     NAVENNSLSDGWNNILPSGTQDYGQAVASWNQYAQDNNLTPEQVQEGMNRIAIGEGPS
                     WGTTYKVHPVVQAGGDVSFIRGYTLSGTIDDNHIS"
BASE COUNT         1494 a         1448 c         1719 g         1145 t
ORIGIN      
        1 tacagggcgg ctgtacagtg caggcaacct gctgcttgac gctcaggact tcagtggtca
       61 ggggcaggtg gtggccaccg gtgatgtcac actgaaactg attgctgccc tcacgaatca
      121 tggtaccctg gccgcaggga aaaccctttc cgtcacgtcg caaaatgcca tcaccaacgg
      181 cggtgtcatg cagggtgatg ccatggtgct cggtgccgga gaggcattca ccaacaatgg
      241 aacgctgact gccggtaaag gcaacagtgt tttcagcgca cagcgtcttt tccttaacgc
      301 accgggttca cttcagggcg gtggcgatgt gagtctgaac agccggagtg atatcaccat
      361 cagtggtttt accggcacgg caggcagtct gacaatgaat gtggccggta ccctgctgaa
      421 cagtgcgctg atttatgcgg ggaataacct gaagctgttt acagaccgtc tgcataacca
      481 gcatggtgat atcctggccg gcaacagtct gtgggtacag aaggatgctt ccggcggtgc
      541 aaacacagag attatcaata attccgggaa tattgagacg catcagggcg atattgttgt
      601 aagaaccggg catcttctga accagcggga gggattttct gccacaacaa caacccggac
      661 taacccctca tccattcagg gaatgggaaa tgctctggtt gatattcccc tttcccttct
      721 tcctgacggc agctatggct atttcacccg tgaagttgaa aatcagcacg gtacgccctg
      781 caacgggcac ggggcatgca atatcacaat ggatacgctt tattattacg cgccgtttgc
      841 tgacagtgcc acacagcgct ttctcagcag ccagaacatc acaacagtaa ccggtgctga
      901 taatccggca ggccgcattg cgtcagggcg taatctttct gctgaggctg aacgactgga
      961 aaaccgggcg tcatttatcc tggcgaatgg ggatatcgca ctctcgggca gagagttaag
     1021 caatcagagc tggcagacgg ggacagagaa tgaatatctg gtataccgct acgacccgaa
     1081 aacgttttac ggtagctatg caacaggctc tctggataaa ctgcccctgc tgtcaccgga
     1141 atttgaaaac aataccatca gattttcact ggatggccgg gaaaaagatt acacgcccgg
     1201 taagacgtat tattccgtta ttcaggcggg cggggatgtt aagacccgtt ttaccagcag
     1261 tatcaataac ggaacaacca ctgcacatgc aggtagtgtc agtccggtgg tctctgcacc
     1321 tgtactgaat acgttaagtc agcagaccgg cggagacagt ctgacacaga cagcgctgca
     1381 gcagtatgag ccggtggtgg ttggctctcc gcaatggcac gatgaactgg caggtgccct
     1441 gaaaaatatt gccggaggtt cgccactgac cggtcagacc ggtatcagtg atgactggcc
     1501 actgccttcc ggcaacaatg gatacctggt tccgtccacg gacccggaca gtccgtatct
     1561 gattacggtg aacccgaaac tggatggtct cggacaggtg gacagccatt tgtttgccgg
     1621 actgtatgag cttcttggag cgaagccggg tcaggcgcca cgtgaaacgg ctccgtcgta
     1681 taccgatgaa aaacagtttc tgggctcatc gtattttctt gaccgcctcg ggctgaaacc
     1741 ggaaaaagat tatcgtttcc tgggggatgc ggtctttgat acccggtatg tcagtaacgc
     1801 ggtgctgagc cggacgggtt cacgttatct caacggactg ggttcagaca cggaacagat
     1861 gcggtatctg atggataacg cggccagaca acagaaagga ctgggattag agtttggtgt
     1921 ggcgctgaca gctgaacaga ttgctcagct tgacggcagc atactgtggt gggagtcagt
     1981 caccatcaac ggacaaacag tcatggtccc gaaactgtat ctgtcgccgg aagatatcac
     2041 cctgcataac ggcagcgtta tcagcgggaa caacgtgcaa cttgcgggcg gcaatatcac
     2101 caacagcggc ggcagcatca acgcacagaa cgacctctcg ctcgacagtt ccggctatat
     2161 cgacaacctg aatgcggggc tgataagcgc gggcggtagc ctggacctga gcgccatcgg
     2221 ggatatcagc aatatcagct cagtcatcag cggtaaaacc gtacaactgg aaagcgtgag
     2281 tggcaacatc agcaatatca cccggcgtca gcaatggaat gcgggcagtg acagccaata
     2341 tggtggtgtg catctcagcg gtacggacac cggtccggtt gcgaccatta aaggcactga
     2401 ttcactttcg ctggatgcag ggaaaaacat tgatattacc ggggcaacgg tctcgtccgg
     2461 tggagacctt ggaatgtctg cgggtaatga tatcaacatt gccgcaaacc tgataagtgg
     2521 gagcaaaagt cagtccggtt tctggcacac tgatgacaac agttcatcat ccaccacctc
     2581 acagggcagc agcatcagcg ccggcggtaa cctggcgatg gctgcaggcc ataatctgga
     2641 tgtcacggca tcctctgttt ctgccgggca cagcgccctg ctttctgcag gtaacgacct
     2701 gagtctgaat gcagtcaggg aaagcaaaaa cagtcgcaac ggcaggtcag aaagtcatga
     2761 aagccacgca gctgtgtcca cggtgacagc gggcgataac ttcctccttg ttgccggtcg
     2821 tgatattgcc agtcaggctg ccggtatggc tgcggaaaat aacgtggtca tccggggcgg
     2881 acgtgatgtg aacctggtgg cagagtctgc cggcgcaggc gacagctata cgtcgaagaa
     2941 aaagaaagag attaacgaga cagtccgtca gcagggaacg gaaatcgcca gcggtggtga
     3001 caccaccgtc aacgcaggac gggatatcac cgctgttgcg tcatccgtta ccgcaaccgg
     3061 caatatcagc gtgaatgccg gtcgtgatgt tgccctgacc acggcgacag aaagtgacta
     3121 tcactatctg gaaacgaaga aaaaaagcgg aggttttctc agtaagaaaa ccacccgcac
     3181 catcagtgag gacagtgcca cccgtgaagc aggctccctg ctgtcgggga accgcgtgac
     3241 cgttaacgcc ggtgataacc tgacggtaga gggttcggat gtggtggctg accgggatgt
     3301 gtcactggcg gcgggtaacc atgttgatgt tcttgctgcc accagtacag atacgtcctg
     3361 gcgctttaag gaaacgaaga aatccggtct gatgggtacc ggcggtattg gtttcaccat
     3421 tggcagcagt aagacaacgc acgaccggcg cgaggccggg acaacgcaga gtcagagtgc
     3481 cagcaccatc ggctctactg ccggtaatgt cagtattacc gcgggcaaac aggctcatat
     3541 cagcggttcg gatgtgattg cgaaccggga tatcagcatt accggtgaca gtgtggtggt
     3601 tgacccgggg catgaccgtc gtactgtgga cgaaaaattt gagcagaaga aaagcgggct
     3661 gacggttgcc ctttccggca cggtgggcag tgccatcaat aatgcggtta ccagtgcaca
     3721 ggagacgaag gagagcagtg acagccgtct gaaagccctg caggccacaa agacagcgct
     3781 gtctggtgtg caggccggac aggctgcgac aatggcctcc gcaaccggtg acccgaatgc
     3841 gatgggagtc agcctgtcgc tcaccaccca gaaatcgaaa tcacaacaac attctgaaag
     3901 tgacacagta tccggcagta cgctgaatgc cgggaataat ctgtctgttg tcgcaaccgg
     3961 taaaaacagg ggcgataacc gcggagatat tgtgattgcc ggaagccagc ttaaggccgg
     4021 tggtaacaca agcctggatg ccgcgaatga tattctgttg agtggcgccg caaacacaca
     4081 aaaaacaacg ggcaggaaca gcagcagtgg cggtggcgtg ggtgtcagta tcggtgccgg
     4141 tggtaacggt gccggtatca gcgtctttgc cggcgttaat gcggcaaaag gcagcgagaa
     4201 aggtaacggt actgagtgga ctgaaaccac aacagacagc ggtaaaaccg tcaccatcaa
     4261 cagtggtcgg gatacggtac tgaacggtgc tcaggtcaac ggcaacagga ttatcgccga
     4321 tgtgggccac gacctgctga taagcagcca gcaggacacc agtaagtacg acagtaaaca
     4381 gaccagcgtg gctgccggcg gcagttttac ctttggctcc atgaccggct caggttacat
     4441 cgctgcctcc cgggataaga tgaagagccg ctttgactcc gttgctgaac aaaccggaat
     4501 gtttgccggt gatggtggct tcgacattac agtgggcaga catacccaac tggatggtgc
     4561 ggtgattgcc tccactgcca caccggataa aaaccacctg gataccggaa cgctggggtt
     4621 cagtgacctt cacaacgaag cggattataa agtcagtcac agtggaatca gtctgagcgg
     4681 tggtggcagc ttcggggata aatttcaggg taacatgccg ggtggcatga tatccgccgg
     4741 aggtcacagc ggacatgcgg aaggaacgac tcaggccgca gtggcagaag gcactatcac
     4801 catccgggac agggacaatc agaagcagaa tctggcgaac ctgagccgtg accctgcgca
     4861 cgctaatgac agtatcagcc cgatatttga caaggagaaa gagcagaggc gtctgcagac
     4921 agtggggctt atcagtgaca taggcagtca ggtggcggat attgcgcgta cacagggaga
     4981 actgaatgcg ttgaaggccg cgaaggaagc aaccggcgaa acacttcctg ctaatgcaac
     5041 agagaaacaa cgtcaggaat atcttgcgaa actgcgtgac acgcaggctt acaggaatga
     5101 aatggcgaag tatggtaccg gcagtgaaat tcagcggggt atccaggccg cgacagcggc
     5161 gcttcagggg ctggcggggg gtaatttagc aggtgctctg gccggagctt cagcaccgga
     5221 actggcacat ctgctgaaat caacagaaaa agacccggca gtaaacgcca ttgctcacgc
     5281 cattctgggc ggcgcagtgg cggcaatgca gggtaataat gtcgctgccg gtgcagcagg
     5341 cgcagcaacg ggagagctgg cagccagagc gattaccgga atgctgtatc ctggcgtgaa
     5401 gcagtcagac ctcagtgaag agcagaagca gacgataagt acgctggcaa ctgtgtcagc
     5461 agggctggcc tgcggtctta ccggaaacag caccgcatca gcagcagtgg gagcacaaag
     5521 cgggaagaat gctgttgaga ataactcact cagtgatggc tggaacaata tcctgccatc
     5581 aggaacacag gattacggac aggccgtagc ctcgtggaat cagtatgctc aggacaataa
     5641 tctgacaccg gagcaggttc aagaagggat gaatcgtata gctattggtg aagggccttc
     5701 ttgggggaca acatataaag tacacccggt agttcaggct ggcggtgatg ttagctttat
     5761 ccggggttat actttaagcg gaactataga tgacaatcat atttca
//
LOCUS       DAZFRN010000241         5747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFRN010000241 DAZFRN010000000
VERSION     DAZFRN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.243"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            364..1236
                     /locus_tag="ACHKUB_004813"
     CDS             364..1236
                     /locus_tag="ACHKUB_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6657714.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1608..4727
                     /gene="agn43"
                     /locus_tag="ACHKUB_004814"
                     /gene_synonym="flu"
     CDS             1608..4727
                     /gene="agn43"
                     /locus_tag="ACHKUB_004814"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO6657715.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASSD
                     NNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHGS
                     AQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGYN
                     GQATLNVTF"
     gene            4848..>5747
                     /locus_tag="ACHKUB_004815"
     CDS             4848..>5747
                     /locus_tag="ACHKUB_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657716.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEAND"
BASE COUNT         1371 a         1543 c         1615 g         1218 t
ORIGIN      
        1 gcactcttat aaaaaacaca cagaagaagt aagataatta taaacgtaaa tttaatatct
       61 cccactgaca tctcccccac aatacactaa aaaatactca cagcagtaaa cacagacaca
      121 cctgataatc atacagccga taaatatttt cccctttacg gagaaatcag cctgctgttt
      181 aacaataacg gcgtattttc atggaaggag atcacccagt aacggataag tcctgatctc
      241 cttcatgtta atacgcaaca atcccatcga ttcaaaacaa tttcagacag atattatctc
      301 cgtgaatccc ccgccacctt tccggtgcgc ggggttttgt cttttttcac cgggaataca
      361 tgtatgaatc cgtctgatgc cattgaggca attgaaaaac cgctctcctc cctgccttac
      421 tcactttccc gtcacatcct ggaacatctg cgcaaactca ccagtcacga acccgtgatt
      481 ggcattatgg gtaaaagcgg ggccggtaaa tcctcactct gtaatgcact gtttcagggg
      541 gaggtcaccc cggtcagtga tgttcacgcc ggcacccggg aagtgcggcg cttccgtctg
      601 agtggccacg gtcacagcat ggttatcact gacctgcccg gggtgggcga gagccgggac
      661 agggatgcag agtatgaagc cctgtaccgt gacattctgc ccgaactgga cctggtgctg
      721 tggctgatta aagccgatga ccgcgccctg tctgtggatg agtatttctg gcgacacatc
      781 ctgcaccggg gacatcagca ggtgctgttt gtggtgacgc aggccgacaa aacggagccc
      841 tgccatgaat gggatatggc cggcattcag ccttctcccg cacaggcaca gaacattcgg
      901 gagaaaacgg aggcggtatt ccgtctgttc cggcccgtac atccggttgt tgccgtatcg
      961 gcccgcaccg gctgggaact ggatacgctg gtcagtgcgc taatgacagc acttcccgac
     1021 catgccgcca gtcccctgat gacccgcctg caggacgagc tgcgcacgga atctgtccgc
     1081 gctcaggccc gtgaacagtt taccggtgcg gtggaccgga tatttgacac ggcggagagc
     1141 gtctgtgttg cctctgttgc ccgtacggtt ctgcgtgccg tccgtgacac ggtggtctct
     1201 gttgcccgcg ctgtatggaa ctggattttc ttctgaacag tctgccaggg aaatgcctga
     1261 taacattacg ttaaccaact gaaaacatga cacaaacccc tcatactcct cctgtttctc
     1321 ttgcgtgacc gccttgtcgt taatagaata aaacgatcga taaaacaggt atcgatcgtt
     1381 gctatcgatc gtttatagtg gttaatggtc tgcgaaaagg cagctgaatc tcttcatcat
     1441 gcagaacgga attgcacaca acggactgat acttctctgg cggatgagag aggggaagca
     1501 tttatgtccg ggaaaaacca ctttatctga gcatgctgtt ttgtacctgc cggtatccac
     1561 atttgtgggt accggctttt ttattcaccc tctgtaagga aaagctgatg aaacgacatc
     1621 tgaacaccag ctacaggctg gtatggaatc acattacggg caccctggtg gtggcctccg
     1681 aactggcccg ctcacgggga aaacgcgctg gtgtggcggt tgcgctgtct cttgctgctg
     1741 tcacatcagt cccggcactg gctgctgaca aggttgtaca ggcgggagaa accgtgaacg
     1801 acggaacact gacaaatcat gacaaccaga ttgtcctcgg tacggccaac ggaatgacca
     1861 tcagtaccgg tctggagtat gggccggata acgaggccaa taccggcgga caatggatac
     1921 aaaatggcgg tatcgccaac aacactactg tcaccggtgg tggtcttcag agagtgaatg
     1981 ccggaggaag cgtttcagac acggttatca gtgccggagg cggacagagc cttcaggggc
     2041 aggcagtgaa caccactctg aacggcggtg agcagtgggt acatgaaggc gggattgcaa
     2101 cgggtaccgt cattaatgag aagggctggc aggccatcaa atccggcgca gtggcaaccg
     2161 acacggttgt gaataccggc gcggaagggg gaccggatgc ggaaaatggt gataccgggc
     2221 agaccgtcta cggagatgcc gtacgcacca ccatcaataa aaatggtcgt cagattgtgg
     2281 ctgctgaagg aacggcaaat accactgtgg tttatgccgg cggcgaccag actgtacatg
     2341 gtcacgcact ggataccacg ctgaatgggg ggtaccagta tgtgcacaac ggcggtacgg
     2401 cgtctggcac tgttgtaaac agtgacggct ggcagattgt caaaaacggg ggtgtggccg
     2461 ggaataccac cgttaatcag aagggcagac tgcaggtgga cgccggtggt acagccacga
     2521 atgtcaccct gaagcagggc ggcgcactgg ttaccagtac ggctgcaacc gttaccggca
     2581 taaaccgcct gggagcattc tctgttgtgg agggtaaagc tgataatgtc gtactggaaa
     2641 atggcggacg cctggatgtg ctgaccggac acacagccac taatacccgc gtggatgatg
     2701 gcggaacgct ggatgtccgc aacggtggca ccgccaccac cgtatccatg ggaaatggcg
     2761 gtgtactgct ggccgattcc ggtgccgctg tcagtggtac ccggagcgac ggaaaggcat
     2821 tcagtatcgg aggtggtcag gcggatgccc tgatgctgga aaaaggcagt tcattcacgc
     2881 tgaacgccgg tgatacggcc acggatacca cggtaaatgg cggactgttc accgccaggg
     2941 gcggcacact ggcgggcacc accacgctga ataacggcgc catacttacc ctttccggga
     3001 agacggtgaa caacgatacc ctgaccatcc gtgaaggcga tgcactcctg cagggaggct
     3061 ctctcaccgg taacggcagc gtggaaaaat caggaagtgg cacactcact gtcagcaaca
     3121 ccacactcac ccagaaagcc gtcaacctga atgaaggcac gctgacgctg aacgacagta
     3181 ccgtcaccac ggatgtcatt gctcagcgcg gtacagccct gaagctgacc ggcagcactg
     3241 tgctgaacgg tgccattgac cccacgaatg tcactctcgc ctctggtgcc acctggaata
     3301 ttcccgataa cgccacggtg cagtcggtgg tggatgacct cagccatgcc ggacagattc
     3361 atttcacctc cacccgcaca gggaagttcg taccggcaac cctgaaagtg aaaaacctga
     3421 acggacagaa tggcaccatc agcctgcgtg tacgcccgga tatggcacag aacaatgctg
     3481 acagactggt cattgacggt ggcagggcaa ccggaaaaac catcctgaac ctggtgaacg
     3541 ccggcaacag tgcgtcgggg ctggcgacca gcggtaaggg tattcaggtg gtggaagcca
     3601 ttaacggtgc caccacggag gaaggggcct ttatccaggg gaataagctg caggccggtg
     3661 cctttaacta ctccctcaac cgggacagtg atgagagctg gtatctgcgc agtgaaaatg
     3721 cttatcgtgc agaagtcccc ctgtatgcct ccatgctgac acaggcaatg gactatgacc
     3781 ggattctggc aggctcccgc agccatcaga ccggtgtaag cggtgaaaat aacagcgtcc
     3841 gtctcagcat tcagggcggt catctcggtc acgataacaa cggcggtatt gcccgtggag
     3901 ccacgccgga aagcagcggc agctatggct tcgtccgtct ggagggtgac ctgctcagaa
     3961 cagaggttgc cggtatgtct gtgaccgcgg gggtatatgg tgctgctggc cattcttccg
     4021 ttgatgttaa ggatgatgac ggctcccgtg ccggcacggt ccgggatgat gccggcagcc
     4081 tgggcggata cctgaatctg atacacaacg cctccggcct gtgggctgac attgtggccc
     4141 agggaacccg ccacagcatg aaagcctcat cggacaataa cgacttccgc gtccggggct
     4201 ggggctggct gggttcgctg gaaaccggtc tgcccttcag tatcactgac aatctgatgc
     4261 tggagccgca actgcagtac acctggcagg gactctccct ggatgacggc caggataacg
     4321 ccagttatgt gaagttcggg catggcagtg cacaacatgt gcgtgccggc ttccgtctgg
     4381 gcagccacca cgatatgaac tttggtaaag gcacctcatc ccgtgacacc ctgcgcggca
     4441 gtgccaaaca cagtgtgcgt gaactgccgg tgaactggtg ggtacagcct tctgttatcc
     4501 gcaccttcag ctcccggggg gacatgagca tgggtacagc cgcagccggc agtaacatga
     4561 cgttctcacc gtcacagaat ggtacgtcac tggacctgca ggccggactg gaagcccgtg
     4621 tccgggaaaa tatcaccctg ggcgttcagg caggttatgt ccacagcgtc agcggcagca
     4681 gcgctgaagg ctataacggt caggccacgc tgaatgtgac cttctgacag aaccatcgcc
     4741 tctctgtggt cccggtcatc atgatcggga cccggaccgg cgcaacggat attcaacgcc
     4801 acattcgctg gcattaacaa taacatgata ttcatcacgg agtgactatg ttacagatag
     4861 tcggcgcgct gatcctgctg atcgcaggat ttgccattct tcgccttttg ttcagagcat
     4921 taatcagcac ggcttctgcg ctggcagggc tcatattgct gtgtctgttc ggcccggcct
     4981 tactggctgg ctatatcacc gaacgcataa cccggttgtt ccatattcgc tggctggcag
     5041 gcgtatttct gacgattgcc ggaatgatca tcagcttcat gtggggactt gatggtaaac
     5101 atatcgcgct ggaggctcac acctttgact ctgtgaaatt tattctgacc accgctctcg
     5161 ccggtggtct gctggctgtt cccctgcaga tcaaaaacat tcagcagaac gggatcacac
     5221 cagaagatat cagcaaggaa attaacgggt attactgctg tttttatact gcctttttcc
     5281 ttatggcgtg ttctgcatgc gcaccattga tcgcgttaca gtacgatatt tcaccgtcac
     5341 tgatgtggtg gggcgggttg ttgtactggc tggctgcatt agtgacgctg ctatgggcgg
     5401 ccagccagat ccaggcgctg aaaaaactga cctgtgccat cagccagaca ctggaagaac
     5461 aaccggtgct caacagtaaa tcgtggctga ccagtttgca aaacgattac agccttcctg
     5521 actcactgac ggagcgcatc tggctgacgc tcatttctca acggatttcc cggggagagc
     5581 tgagggaatt tgaactggca gacggaaact ggttactgaa caatgcctgg tatgaaagaa
     5641 acatggcagg gtttaacgaa cagttgaaag agaacctgtc attcacacct gatgaactga
     5701 aaacgctctt ccggaaccgc ctgaatttat caccggaagc gaatgac
//
LOCUS       DAZFRN010000242         5712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFRN010000242 DAZFRN010000000
VERSION     DAZFRN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.244"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(1040..1570)
                     /locus_tag="ACHKUB_004816"
     CDS             complement(1040..1570)
                     /locus_tag="ACHKUB_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6657717.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAVMAITALK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            2240..2971
                     /locus_tag="ACHKUB_004817"
     CDS             2240..2971
                     /locus_tag="ACHKUB_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6657718.1"
                     /translation="MNQPIHNDYWLSRFESILNSALVQHRAVSLIRVDLRFPEYMPAT
                     IMDPDPDSAVISRFFESLKAKIQAYQRKKRCANQRVHATSLGYFWCREFGKMYGRKHY
                     HVILLLNKDTWCSLGDFTVSSSLATLIQEAWCSALRIEPWLGDGLVHFSRWTPSRKPT
                     SPAAPPSSDDTPLPSGCSDTRKASDKKSGGSAVLWVTRGDKEAVQKALERARYLVKNE
                     TKLHNGSGQRNYGCSRGPGRLLDGR"
     gene            3354..3482
                     /locus_tag="ACHKUB_004818"
                     /pseudo
     CDS             3354..3482
                     /locus_tag="ACHKUB_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4036..5388)
                     /locus_tag="ACHKUB_004819"
     CDS             complement(4036..5388)
                     /locus_tag="ACHKUB_004819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657719.1"
                     /translation="MDSGFVDLDILLTRIRHAQSKTYFLDAVKAYKAGALRGALTSAW
                     VALVYDLIAKYRELSAMGDAAATAFLNTWDNATKSGDIPKLLQLEGGILEDATANAQV
                     VNRLARTHLDRLREDRHLCAHPAFSAEADLFEPSHELVRLHLVNAVEFVLSQEPLQGK
                     AIFELYDVDVQSPGFPTDSKRILDYVEQRYLTRVRAQNISNFGTILAKSLLKGVPEQW
                     ETLYHQIITSLVAVRDRASIAWPNVSLSIVRLIDNLEPRHRPRAIAFIAAFNEFWMQL
                     QEPTRTVLLETIENANPENITDYRILTGVTFVPFQASLQRLITGLTQQQLASAIIFNP
                     LAELWDKAIEYYAESGSWRGSEGNFISLVIPFSGRLSQQQFDQLLDAIISNTQNWDAA
                     ETPKLLLGMLRNSLPANLPTIDARARFYHHLDRHHCINRYGDVLTILQTDGWLPLPQK
                     "
BASE COUNT         1562 a         1192 c         1268 g         1690 t
ORIGIN      
        1 taatggtgct gatgatccgc cagtttagta tggttatatg atttgatggt taatgcttac
       61 agataatcac actttcttat gaatagtata aatgagcatg ccatagtcgt ttgttcagag
      121 ataaaattta ctgaatgagc ttatgttgtg gttatctgta cttaccggat acgacgactt
      181 atatccctca cattatatgg cttaactttt ctggggtaaa gcgatggagt tacagacgct
      241 gattacgatg gtttactgtc tcgttatcac gggatttctg ctgtactgaa tacgtgaaca
      301 ggaaaataac tccagagtaa atctgctctg tttttaggac agaaaactcc atgctggctg
      361 taaaagtgaa aaattaacac ccagtatgta tggaagtctg aagatgttga gaatgtcggt
      421 atgatactat atacacgtat tttctactaa gtgttttacc agacatacac gtattttcat
      481 aaacacttct acaagtgttt tcgttaagca ttgctataca catccagttt agtaaatctt
      541 tacgttaact aaatcagttt aatgctgctg taaaaccttt ctgtttaagc aatcaatctg
      601 ctattcgatt ttactgatgt tacgtttaac ctttccttag atttcgagtt tcgacgttag
      661 cttgagaaaa gtatctttac gtaaagccca gtgaattgac tcaaatccga ttgaggtaac
      721 ctgatgcgat ttactgtgag tttgcgataa cactgttaaa tggcttatgg atgattttgt
      781 aacggcgaga ttgataaggt gattaactgg ccctggtgtt ttttctcagc gtaaggtgat
      841 tatcttcatc ataaggctct tttcagaaaa agtgtgatga ttggttgtgg tgttttgatt
      901 cagatgagga tctgccaatg attatgatgt attgtaagaa ataacgcctt cagaatgttg
      961 gagcatgcac ttacacaaac tgtttttcta aaattaaggg aacagtttaa agagtataaa
     1021 ctgttcatct tcttcaggat taagctacat tgttgttatg actgacagga ttgttttctt
     1081 cccctgactc tgggcttaac agtccctggc gttgtagata ttcactgact gacattccag
     1141 aactacggat cctgtcagag attttacgaa attgatcctt ccattctgca ggtaatttct
     1201 taatttgtct gactttacag gctacactgt acggggttcg ccctaatttc tctgccagcc
     1261 agaaaaggtg tttaccaatt tcatcgtccg gtacatcctc cagaacatct cgcaataaac
     1321 tctcgtctgc ctcattccat gccacaccag catttgggaa acgaggatcg atctctcttt
     1381 ccttcttctc cttcttactt tgctcttttc gggatgatac agatacgaca gaacgttctt
     1441 ttagcgctgt aattgccata actgcattga gcagagatct gcgctccaca tcgttcagca
     1501 tttttccttc acagatcaaa tcattcagga tgtcaatgac tagattcaga ctttcttctg
     1561 ttagcttcat atttcagacc ttgaagtatg tagataatca gcacaattac taatgtgata
     1621 aatatcagaa gataatttac aggtaaaccg gaaaatacat ctgaagaata aaggcctcag
     1681 cttaacgttt cagccagttt gtgagctgat tgaggtacgg cgatgacatt aacgggaatt
     1741 actcccctat agctctgagc ttatttttca ccctggcaac atatggtggc tactgcgcat
     1801 ggttttggag tagatatctt actactcgta gaattgtgct tactggtcag gccagcgcac
     1861 aggcattccg tgcaatcaat agaacactgg ttttttagtc ttccgttacc catcaggatg
     1921 ttagtgcaga ttccggtgta ttcgatcagt tgttcggcga atcagcgatc gatcacgatg
     1981 cgatttcgta tgttagggat gctggtatga ttactcgctg aaaaataatg tgaaaaggca
     2041 gtttttcttt agacatttag ctcattcatg ctgttgtttt acgttttgct gtcgtgtgca
     2101 ggattatctt ttcgttacgg gacgattcat tccgttttaa tcaggagcta ttggcgttgc
     2161 tcattggtgg gatgccgtaa agttttaccg cggcgattaa tgatgtgaag tcaatccaaa
     2221 tcaacggaga tctctcatca tgaatcaacc aatacacaat gattactggt tatcccgttt
     2281 tgaaagtatt ctcaacagtg ccctggtgca acaccgtgcc gtctcgttaa tccgggtgga
     2341 tttacgtttt cctgagtata tgcctgccac catcatggat cccgatccgg attcagcggt
     2401 gatttctcgt tttttcgaat ccctgaaagc caaaattcag gcttaccagc ggaaaaaaag
     2461 atgtgccaat cagcgtgtgc atgcaaccag cctgggttat ttctggtgcc gggagttcgg
     2521 caagatgtat ggcaggaaac attatcacgt gatattactg ctcaacaaag atacctggtg
     2581 ctcgctcggg gatttcaccg tttcttcttc gctggcgacg ctgatccagg aggcatggtg
     2641 cagcgccctg cgtattgagc cctggctggg tgatgggctg gtccattttt ctaggtggac
     2701 tccttcccgt aaaccgacat cacctgctgc tcccccttct tccgatgaca cgcctttacc
     2761 aagtgggtgt tctgataccc ggaaggcttc tgacaaaaag tctggtggat ccgctgtgct
     2821 ctgggtcacg cgtggtgaca aggaagcggt gcagaaagcc ctggagagag cccgttatct
     2881 cgtgaaaaat gaaacgaagt tgcataacgg ttccgggcaa cgtaattatg gttgcagtcg
     2941 tgggccgggg cgtctactgg atggcaggtg aacccggtaa aacggcatcc ggtgcctgag
     3001 catacgtcac gtaattaact gtcttgacca gcaaaattat ggagtaacct gatggcagga
     3061 ttcattacat ttctttgttc tttctgagca tcgcattcag gatggcacta aagagtttga
     3121 gcaacttgca atgaaaaggg aattttcctc gcatgaaacg gccagcattg aactgtgcag
     3181 cactctcttt tgcttgcttg actattgggg aattgatcga gtgatgtttt cttccggcat
     3241 caataacaat ctaaatagct gctcggaata gtagatcact caaggggaac tcagtccggt
     3301 ttatgcgatc tgatcaatcg ctgaatatcc caaatcacca gccggactga gtaatgccga
     3361 tcatagtacc aatacccaga gccgaacggc acctgatgca gaaaactatc cataaaacaa
     3421 atgacaaaaa ccatgcccgg cggctcaccg caatgctgat gctacatcgt ggtgacaccg
     3481 tcagatttag tctacgtcat gagcaactag aggttacaga aacacagttt atagagtttg
     3541 cagatgcgat tgcagctttc tggaatgata tcctaacaca ctgtatgcga tcagctattc
     3601 gggaagaacc tgaatggata aatggcgaat ataaaaacat gagaagaaag ttaaaatgct
     3661 tcaggactaa aattcttgat aatagcaagt gtatcttaca tgtagacatt tgcctgccgt
     3721 gttttttagc taccttggtt gcgagaacaa ttgcgaactt ctgcacctcg ctcacagcgg
     3781 acctactgct aagttagtct gtccgcttct tgccaagaac ggccgtttct ctcaataaag
     3841 caatataatt cagtagggta taaatcaccg tggccagata gaatttatta agagattcca
     3901 acgtaccaag aatgatggaa gttgagcgag tcgtcgcaca tggtattcgg atcaagataa
     3961 ctatggcatg tcttttctga tattatcaac atgccggtat ttcatcatta atttcgttta
     4021 gaccgcctta gtagattatt tctgcggtag aggaagccag ccgtcggttt gtagaattgt
     4081 gagtacatca ccatacctat taatacagtg atggcgatct aggtgatgat aaaaacgagc
     4141 acgtgcatct attgttggta ggttggcggg taaagaattg cggagcattc ctaggagaag
     4201 cttaggcgtc tcagcggcat cccaattctg cgtattggaa atgatagcat ctagaagttg
     4261 gtcgaactgt tgctgactaa ggcggccaga aaacggtatg acgagagaaa taaagttccc
     4321 ctcagatcca cgccagctac ctgattctgc atagtattct attgccttat cccaaagttc
     4381 agcaagtggg ttaaagatga tcgctgaggc caactgctgt tgagttagtc ccgttattag
     4441 cctttgcagc gaggcttgaa acggtacgaa agttacacca gtcaaaatac ggtaatcagt
     4501 gatattttct ggattggcat tttcaatagt ctctagcaag actgttcgcg ttggctcctg
     4561 aagctgcatc caaaactcat taaatgcggc aataaatgcg atcgctctag gccgatgtcg
     4621 tggttctaga ttatcaataa gcctaacaat tgacagtgaa acgtttggcc aagcaatgct
     4681 ggcgcggtct cgtacggcga ctaatgatgt aataatctgg tgataaagcg tttcccattg
     4741 ttctggcaca cctttgagaa gtgatttggc gagaatcgtg ccgaagttac tgatattctg
     4801 agcgcgtacg cgagtaagat aacgttgttc tacataatca agaatgcgtt tactgtccgt
     4861 aggaaaaccg ggcgattgaa catcaacgtc atataactcg aatattgctt ttccctgtaa
     4921 aggttcttgc gataggacga attccacagc atttactaaa tgaagtcgca ctaactcatg
     4981 agaaggttcg aatagatctg cttctgctga gaatgctgga tgcgcgcaaa gatgcctatc
     5041 ctcgcgaaga cggtctaagt gagttcgggc taaccggttg actacctgag cattggccgt
     5101 tgcgtcttca agaatgcccc cctcaagttg gagaagttta gggatgtcac ctgatttagt
     5161 cgcgttgtcc caagtattaa gaaatgcagt cgctgctgca tcgcccatgg cactaagctc
     5221 acgatatttg gctatcagat cgtagacgag agcaacccaa gcggaagtta gtgcccccct
     5281 caacgcgccc gctttgtagg ccttaacggc atcaagaaag tatgttttcg actgggcatg
     5341 cctaattcgt gtaagaagta tatcgagatc tacgaatcca ctgtccataa tttaaattca
     5401 atgagttaaa gtgatcgagg atcatatcac aacattctgt aaccaacctc taagattatc
     5461 gtgcaataat gctcaaaatt cagttttttc atcagctagc ctagagggac aatcaaactt
     5521 tcgctcctca ctcatagcag acttttacat agattctact cctaaaatgg agcgacctct
     5581 gtttcaaaat tagtttgtaa gtaaagagaa tacctggagg ccaataatcc gggcattggc
     5641 aacaacgata aatatctata taaccaatac aatactatcg catcactaaa ttgatgtgtc
     5701 tcggtaggtg at
//
LOCUS       DAZFRN010000243         5696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFRN010000243 DAZFRN010000000
VERSION     DAZFRN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.245"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            96..812
                     /gene="nanC"
                     /locus_tag="ACHKUB_004820"
     CDS             96..812
                     /gene="nanC"
                     /locus_tag="ACHKUB_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO6657720.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            832..1938
                     /gene="nanM"
                     /locus_tag="ACHKUB_004821"
     CDS             832..1938
                     /gene="nanM"
                     /locus_tag="ACHKUB_004821"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO6657721.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2003..2983
                     /gene="nanS"
                     /locus_tag="ACHKUB_004822"
     CDS             2003..2983
                     /gene="nanS"
                     /locus_tag="ACHKUB_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6657722.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(2991..3641)
                     /locus_tag="ACHKUB_004823"
     CDS             complement(2991..3641)
                     /locus_tag="ACHKUB_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6657723.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            3778..3921
                     /locus_tag="ACHKUB_004824"
                     /pseudo
     CDS             3778..3921
                     /locus_tag="ACHKUB_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4585..4713)
                     /locus_tag="ACHKUB_004825"
                     /pseudo
     CDS             complement(4585..4713)
                     /locus_tag="ACHKUB_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4689..4838)
                     /locus_tag="ACHKUB_004826"
                     /pseudo
     CDS             complement(4689..4838)
                     /locus_tag="ACHKUB_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(4923..5120)
                     /locus_tag="ACHKUB_004827"
     CDS             complement(4923..5120)
                     /locus_tag="ACHKUB_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6657724.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(5132..5620)
                     /locus_tag="ACHKUB_004828"
     CDS             complement(5132..5620)
                     /locus_tag="ACHKUB_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6657725.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(5617..>5696)
                     /locus_tag="ACHKUB_004829"
     CDS             complement(5617..>5696)
                     /locus_tag="ACHKUB_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFO6657726.1"
                     /translation="ATGLMTRDNYRTVNNITLGKHPEKR"
BASE COUNT         1604 a         1258 c         1344 g         1490 t
ORIGIN      
        1 ctataagcct ggcaaaaaaa tattacacaa cataaatgct aattgtttat gcgggctttg
       61 tattgctttc tgtatcctac aaatgagtga aatttatgaa aaaggctaaa atactttctg
      121 gcgtattatt actgtgcttt tcgtccccat taatttctca ggctgcgaca ctggacgtac
      181 gtggtggata tcgtagtgga agccacgcct atgagactcg actcaaagtc agtgagggat
      241 ggcaaaatgg atggtgggca agcatggaaa gtaatacctg gaataccatt catgataata
      301 aaaaggaaaa tgccgcactc aatgatgttc aggttgaagt taattacgcg attaaacttg
      361 atgatcaatg gacggtgcgc ccgggaatgt taacgcattt tagcagcaac ggcacacgct
      421 acggacccta cgtaaaactg tcctgggacg cgacaaaaga tcttaatttt ggcattcgct
      481 atcgttacga ctggaaagct taccgacaac aagacttatc cggtgatatg tctcgtgata
      541 acgttcatcg ttgggatgga tatgtcactt accatattaa tagtgatttc accttcgcat
      601 ggcaaacgac gctatacagc aaacagaacg attatcgcta tgcaaaccat aagaaatggg
      661 cgacggaaaa tgcatttgtt ctacaatacc atatgacgcc cgatattacg ccatacatag
      721 aatatgacta cctggaccgt cagggtgttt acaacggcag agataattta tcggaaaaca
      781 gttatcgcat tggtgtgtca tttaaactgt agtagacagg agacagtcac aatgaataaa
      841 acaataacgg cgcttgctat catgatggct tcatttgccg caaacgcgtc tgtattacct
      901 gaaactcctg tgccatttaa aagtggtacc ggagcaattg ataacgacac tgtctacatt
      961 ggtttaggta gcgcaggtac ggcatggtac aagctggata cacaggccaa agataaaaaa
     1021 tggacagcgt tagctgcatt ccctggtgga ccaagagatc aagcaacctc ggcatttatt
     1081 gatggcaatc tgtatgtgtt tggcggcatt ggcaaaaata gcaagggctt gactcaggta
     1141 tttaatgacg tacacaaata caaccccaaa accaatagtt gggttaaatt gatgtcgcat
     1201 gcaccgatgg gcatggcggg tcatgtgact tttgtacaca acggcaaggc ttatgttact
     1261 ggcggtgtta accagaatat cttcaatggc tattttgaag atctcaacga ggctggaaaa
     1321 gattcagcaa ctatagacaa gatcaatgcc cattattttg acaaaaaagc agaagattat
     1381 ttcttcaata agtttctgtt gtcttttgat ccctcaacac agcaatggag ttacgctggc
     1441 gaatctccct ggtacggaac ggctggtgcg gcggttgtga ataaaggtga taaaacctgg
     1501 cttattaatg gcgaagccaa accaggattg cgaacggatg ccgtatttga acttgatttc
     1561 accggtaata atttaaaatg gaataagctt gctcccgtct catcaccaga tggcgtcgct
     1621 ggcggttttg cggggataag caatgattct cttatatttg ccggaggggc cggattcaaa
     1681 ggttcacgag aaaattacca gaacggaaag aactatgcgc atgaaggcct gaaaaaatca
     1741 tatagcactg atattcatct ttggcataac gggaaatggg ataaatcggg tgaattatcg
     1801 caaggtcggg cctacggagt atcattgccc tggaataata gtctattgat tattggcggt
     1861 gaaactgcag gcggcaaagc ggtgacggat tcagttttga tctctgtgaa ggataataaa
     1921 gtcacagtac aaaattaacg cttcagggcc ccggtaaagg ggccctgcta tcaatttgtc
     1981 atttcaatta cgcgaaattt atatgaacgc aataatatcg cccgattatt actatgttct
     2041 taccgttgct ggtcagtcta atgccatggc gtatggcgaa ggactgccat taccggacag
     2101 ggaagatgcg cctcatccca gaattaaaca attagcgaga tttgcgcata cgcatcccgg
     2161 aggcccgtca tgtcacttta acgacattat tccactgact cactgcccac acgatgttca
     2221 ggatatgcag ggttatcacc atcctctggc aacgaatcat caaacacagt acggcaccgt
     2281 tggccaggca ctgcatattg cacggaaatt actacccttt attcctgata atgcaggggt
     2341 tctcatcgtt ccatgttgcc gtggcggatc ggcttttacc gcgggcagcg aagggacata
     2401 ttcagaacgg cacggagcca gccatgatgc ttgtcgttgg ggaacggata ctccgctgta
     2461 ccaggattta gtcagcagaa cgcgagccgc actggtaaaa aatccgcaga acaaattcct
     2521 cggcgtatgc tggatgcaag gcgaatttga cttaatgacc agtgactacg cgtcacaccc
     2581 tcaacacttt aatcatatgg ttgaagcctt tcgtagggat ctaaaacaat accattctca
     2641 gcttaataat attactgacg caccgtggtt ttgcggcgat accacctggt actggaaaga
     2701 aaatttccct catgcgtatg aagttattta tggcaattat caaaataatg ttttagccaa
     2761 tattattttc gtcgacttcc agcaacaagg tgaaagagga ctgacgaacg cgcctgatga
     2821 agacccggac gatttaagca cgggatatta cggttcagcg taccggtcac cggagaactg
     2881 gacgacggca ctgcgaagca gtcatttcag cacggcagcc cgtcggggga ttatttctga
     2941 caagtttgta gaagcaattt tgcagttttg gcgcgaaagg tgagcgttca ttaatttgat
     3001 ttattcgcat ccatcagcgc ttttaactgc tccggcgtaa acggtggttt gcgtcggtgc
     3061 aacatcgcat ggcaattagg gtacacaggc agcaaatcat taactggatc cagctggtaa
     3121 tcctctttaa tggctgacag cggcaccagg tgatgcacat ggataaagcc ttcagcgata
     3181 tcaccataga tcttcaccag atcaacaccg catacgttgc actggcatcc atgatgttcg
     3241 accgctttat ctcgggcctt tttatcgcgt tcatagcggt ttactgtcac ctttatcgcc
     3301 gcaccttcaa ggtatgtctc ctgcgacggc aactcatccg ggaacagaac gtcggctatg
     3361 ggagccgcag cggctttatc cagatgaata tgaaatccct gctctatgag tagcttgaca
     3421 catttagatt ttattccgcc ggtgaaatcc gcaggggtaa attgcgtccc cgtcatcaat
     3481 gtcgctgcga tgccagcaat cgccttcgag gggtaacgtc ggccatgata ttcaatctca
     3541 tacaaccgcg caggctggaa ctgatgagtt tctcccgcat cccataaatg gatagcttcg
     3601 atgatatacc gccgcggaag atgatcaggg agtttattca tggagaatgt ccttatacgt
     3661 tctcagaaca ggcagcaaaa aaacaaatga gatcaaggta atatatcttc acttgcaggc
     3721 ttgaacagca gaaaatttgc gagcatgatc gcaattattt cactgccagg gatatcaata
     3781 gcttccagta aatcgctaca gcaggcaatt gccaatataa aaatctggca caagggtgaa
     3841 cagcgcgcgc cgcataagcc attgttattg ctatacgtat tagcgggata cctgaatgga
     3901 catccgcgcc ttttcgatta ttgtagtgga acagtctacg aggatgcagt tcgaagtgag
     3961 taagtaaagg ttgctggaaa cgatcaatgc cgccatacaa tgatttttta ggatcaacaa
     4021 tgagtaataa aaactacgaa agtcaccgca aagcgattgt tagtaaaggt ataccacccg
     4081 ctctattaaa taggctcacc aattcggatg ttcaggtgat caataccttc ttaacacgag
     4141 tgagcaaact ggaactatct caacaagaga aagactggat cataaaaatc atctctatgg
     4201 tttagacaat ttcagccttg tagcctttca agggttttgt gtgggtacaa acattatcga
     4261 atcggggtat agccgcagct gtagctgtat ccacatcagt aagttgatgt aggttgactt
     4321 aggttgagcg aaaaatagcg aaaagccttg tgtggcgcgg atttcagtca taaaaaaacg
     4381 tccgttgatg tctattgatg ttccgatggt gcgaaggtgc gaaggtgcga aggccggact
     4441 caaacatgtg acttaacctc atgatttaaa attgttaata ataaacaata tcatcttata
     4501 ccaacacaga taccaacacg aaaaatcact ggttttcggc atcgaaccag gacaatcagc
     4561 tcctgattga gataccgagc tctgttactc gtcatcttcc tcgacaggct tcattgcatc
     4621 aagtagtact cgcaccatcg cgatagaacc aatccggata aaagcatctt ctccatctat
     4681 atctgttaac tgatatcgct cacttgatgc cggataattg tgtcgatttg cagcagtgaa
     4741 ctctttcgta cagccagaat gcggcggatg ctggttgagc agataacgtc cgtattctcg
     4801 ttgaccactt ccggacggta ggctgtgata tcagacataa tcgatattcc tttttgcaga
     4861 taaatgaaaa tgccagccct gttcaggctg gcgtcagtgg aatgaagtct ggtgagtaag
     4921 actcaccggt tctggtgctg aagctgcagt tggcgcaggg tacggatatc ctctctggcc
     4981 tgttcgatac agggcaacag tgctgctgaa tccgttttat cctcattgtt gtcaaagata
     5041 attcccgatt cgcagtcgat attgtcctgc agccacgcaa tcagaatatc cagtgcggtt
     5101 tccgtggtta atgatttcat gttgttcctg gttaccagtt gaaagtgggt aaacccggca
     5161 ggcattcagc gctggcatcc agatgaatga gactgacgcc ataacgtcgg gtaagtgtga
     5221 taaccagacg acggaacgtt tttgacagcc ccaggcgttt aagccgcaga accggatatg
     5281 accacgcatc ggtacgtaac agatatccgc tacctgtgaa atgaatccat tctgattcac
     5341 caacgtcact ggtctgatgc gacagcgagt acagccaggc gttatccttt tccgtgatat
     5401 gtgcggtact gcagcgtatg ccggcaaggg ttgcaaacgg tggtagtgtg caggttgact
     5461 gttggtcgga ttcctccacc acgcggagtg aataaccgtt ttcagcgacc ttgttaatca
     5521 gttcagtgag attaacacca tcgacgtcaa cgacaattcg ccccatgttc agtgcctgca
     5581 cattaattct gtcggcttcc agcatcaggg aaagtttcat cgtttctccg gatgcttacc
     5641 cagggtaatg ttatttaccg ttctgtaatt gtcgcgggtc atcaggccgg tcgccc
//
LOCUS       DAZFRN010000244         5627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFRN010000244 DAZFRN010000000
VERSION     DAZFRN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.246"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(58..444)
                     /gene="ridA"
                     /locus_tag="ACHKUB_004830"
     CDS             complement(58..444)
                     /gene="ridA"
                     /locus_tag="ACHKUB_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO6657727.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(517..978)
                     /gene="pyrI"
                     /locus_tag="ACHKUB_004831"
     CDS             complement(517..978)
                     /gene="pyrI"
                     /locus_tag="ACHKUB_004831"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO6657728.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(991..1926)
                     /gene="pyrB"
                     /locus_tag="ACHKUB_004832"
     CDS             complement(991..1926)
                     /gene="pyrB"
                     /locus_tag="ACHKUB_004832"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO6657729.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1930..2064)
                     /gene="pyrL"
                     /locus_tag="ACHKUB_004833"
     CDS             complement(1930..2064)
                     /gene="pyrL"
                     /locus_tag="ACHKUB_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO6657730.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2345..2740)
                     /locus_tag="ACHKUB_004834"
     CDS             complement(2345..2740)
                     /locus_tag="ACHKUB_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6657731.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(2871..3584)
                     /gene="bdcA"
                     /locus_tag="ACHKUB_004835"
     CDS             complement(2871..3584)
                     /gene="bdcA"
                     /locus_tag="ACHKUB_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6657732.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMSRGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3655..4248
                     /locus_tag="ACHKUB_004836"
     CDS             3655..4248
                     /locus_tag="ACHKUB_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6657733.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            4393..4845
                     /gene="tabA"
                     /locus_tag="ACHKUB_004837"
     CDS             4393..4845
                     /gene="tabA"
                     /locus_tag="ACHKUB_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO6657734.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4968..>5627
                     /locus_tag="ACHKUB_004838"
     CDS             4968..>5627
                     /locus_tag="ACHKUB_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6657735.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KP"
BASE COUNT         1440 a         1302 c         1400 g         1485 t
ORIGIN      
        1 attttgcgtt tgtcatcaga cttaatccgg gcatgatagc ccggatttcc atcaagatta
       61 gcgacgaaca gcgatcgctt cgatctcaat ttttacgtct ttcggcagac gggcaacttc
      121 aacgcaagaa cgtgccggga aggtggcgtt gtgttcggtg aagaaggctt cgtaagtggc
      181 gtttacggtt gcgaagtcgt tcagatcttt cacaaacacg gtggttttaa cgatgtcgcc
      241 cactttcagg cctgcggctt cgacgatcgc ttttacgtta tccagcgact gacgtgcctg
      301 tgcagcgacg tctgccggta cttcgcccgt tttcggattt accgggattt gaccggaggt
      361 gatgatcata ttgcccagat caacgccctg tacgtaagga ccgatagctg ccggtgcatt
      421 ttccgtcgcg atagttttgc tcatgatttc tcctttatta cagcggtaaa aagtctggct
      481 cattataggg agccagactt ttattaccaa ccgcaattaa ttggccagca ccacattatg
      541 ggaaaactct ttttcacagt atttgcattt gagcgcgatg tcattggcgc gttttcgcac
      601 ggcaaagctg gaagaaaccg gttcggcatg gctgatacag ttgctgttcg ggcagaccag
      661 cacattgtcg atgcgctctg gcagactggg gcgcgattta cccaccactt catagttgtc
      721 gatacggtta accgtggctt gcggcgcata caatgccagt tgatctacct gatcttcact
      781 caaaaaggta ttttcgattt tgatcagatc tttgcggccc atctcgccag aaggcaagtt
      841 cagaccgatg gtgatgcgct gatccgtttc ggtcagcttg aacagactca acagcttaaa
      901 accgatctgg gcggggatat ggtcaattac cgtgccgcgt ttaatagctt caacctgcaa
      961 tttattatcg tgtgtcatct ctatttcccc ttacagtacc agatcgcgat tcagaaccag
     1021 tgccagtaac gcctggcgag cgaaaatccc gttgcctgcc tgctggaagt accaggcgtg
     1081 tggcgtttta tcaacatccg tcgcaatctc atcaacacgc ggcagcggat gcagcacttt
     1141 catattggct ttggcgttgt ggagatcgct ggcgcgaaga acaaactgcg ctttcacgtt
     1201 ggcgtactcg gacgggtcca gacgctcttt ttgcacgcgg gtcatgtaca ggatgtctac
     1261 ttccgccatc acttcttcaa tagagctgtg cagactccat gcgatccctt tttcatcgag
     1321 catatccaga atgtattgcg gcattgccag cgcgtccggc gcgatgaagt aaaaacggtt
     1381 gccgtcgaac ttcgctaacg cctgagtcag ggagtgaacg gtgcggccat atttcaggtc
     1441 accaaccatt gcgacgtgga gattgtccag acgcccctgg gtttcctgaa tagtgaataa
     1501 gtccagcaag gtttgcgtcg gatgttggtt ggagccatca ccggcattca gtaccggtac
     1561 attgccggaa aactcggtgg ccaggcgcgc cgcaccttcc tgcggatgac gcatcactat
     1621 cgcatcgacg taagtgctga taaccgaaat ggtatcggcc agcgtttcgc cctttttacc
     1681 cagtgatgta ttggcgctgt cggagaagcc caccacgctg gcacccaggc ggtgcataga
     1741 tgtttcgaaa gagaggcggg tacgggtaga ggcttcgaag aaacagctgg caatgacttt
     1801 gtgcttcaac agctctggtt gcgggtttgc tttcagtttc gccgctgtcg ccagcaccag
     1861 attaaggtca tcgcgactaa ggtcgtttat ggaaatgata tgtttctgat atagcggatt
     1921 agccatcttt tatctcctga cgcctgggca aaaaaaagcc cctcgattga ggggctggga
     1981 atgggtgatc aacgggaaga aaaacggcag gccagcgtct tttttcagac gcggtaagac
     2041 aaaatgtcga acacactgaa ccatacatcc tcccggcaaa ttgtccggca ttatactcat
     2101 cgtcagaagc ggcgcaagca tttgatgcaa tattttgtca gcgcaaacgg tttatttgaa
     2161 ttaaaagtca aggtatatgc atttttattt gatgtgattc tgcaggggaa ctgtcgttcg
     2221 gtatcaataa ttgcagacat tccctgcttt tttctttttc actttcacgc aatcagatat
     2281 gcattttatt ccattcatcc gacttatagg gcggagtgtg aaagcgaacg gctaacacta
     2341 ttgcttactg ctcagggatg cgcgctatca ctttaatttc aaaatcaaag cctgccagcc
     2401 atgtaacacc caccgccgtc cagtttggat aaggtggggc gctaaatatt tcatttttca
     2461 ccgtcatgat gtcttcaaat tggttttctg gatcggtatg gaagctcgta acatcaatga
     2521 tatcgtcaaa agtgcatccc gcagctgcca gggtcgcatg caaattatca aatgccagtc
     2581 tgacttgttg ctgaaaatcg ggttctggtg ttccgtcctc tcgacttcct acttgcccgg
     2641 aaacaaacag caaatcgccg gaacgaatag ccgcagaata acgatgctca gcatatagtg
     2701 aatgtcggcc agcagggaaa acagcggttc tttctaccat ttggttatcc tcaagattta
     2761 cgacatgaac agaagatttc tctttaccgg gagccgcttt tagcggacga cgtgagtaaa
     2821 caaaacccag acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca
     2881 aacgcgccat caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac
     2941 catgcgacca taccagcgac ctcttccggt tgcccatgtc ttttgatagc catcaaacta
     3001 tgcaacatat cgcgcattgg cccgttggcg ggattagcgt cggtatcaat tggccctggc
     3061 tggacgacgt tgatggtgat cccacgcggt ccaaaatcac gggccagccc gcgcgacatg
     3121 ccttgtaggg cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg
     3181 ccattcacgg agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca
     3241 acagaggcat ggtaaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca
     3301 tttaattcca gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat
     3361 gcgccgctct tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact
     3421 gctgttgctc cagtctcttg tgccaggcgt tcagcggcat ctttcgaccc cgcataggtg
     3481 aatcgtacat tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacgg
     3541 ctaccaccga ggatgagaac tgtcttacct gtaaaagcgc ccataaggac tccttgattt
     3601 attatgtaac atgcattaca aaactgtttt aactttctgt caacaagttt tgtaatggtc
     3661 actaaaaaac aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc
     3721 tctgcggcaa aagtgctttt tcaccaaaaa ggtttcgatg ctgtcagtgt tgctgaagtt
     3781 actgattatc ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg
     3841 ttatttagcc gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgtcgatatt
     3901 cttcgtgatg atcgtccggt aggggagtgc ctggctgagg tattaaaaga agcggcgcgc
     3961 agatatagcc aaaacggcgg ctgcgctggc tgtatggttc ttgaaggcat tcatagtcat
     4021 gatccacaag cgcgtgatat tgccgttcaa tattatcacg ccgcagaaac ggccatttat
     4081 gactatatcg ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc
     4141 gtgatgtcag ggctttctgc gaaggcacga gaggggcact ccctcgaaca actctgtgca
     4201 acagctgcac tggcgggggg agcgataaaa actattctca aggagtgatg cgggccttga
     4261 cccgaaaggc gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa
     4321 ggctagcgga attgatcatc ttttcgtata acgatagaaa tgaaacgttg ttttaattaa
     4381 ggagtggaaa agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag
     4441 ttacgccagg cgattgagca tatcaaagca cacgttacgg tagaaacgcc aaagggcaag
     4501 cacgatatcg aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac
     4561 gaagctcgcc gtgcggagta tcatgcccgc tatctcgaca ttcagattgt gttaaaaggt
     4621 caggaaggca tgaccttcag cacgcaacct gcaggcacgc cggataccga ctggttagct
     4681 gataaagaca tcgcattttt gccggaaggc gttgatgaga aaacagttat ccttaatgaa
     4741 ggtgattttg ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg
     4801 ccagcccagg ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta
     4861 aataactcca ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag
     4921 tagaatttgt aatgcttaga cgttcttatt aactcaagga gtccgtcatg agcaaaatat
     4981 caggttggaa tttttctcaa aacattacat cagccgacaa ttgtaaacaa aaaaatgaag
     5041 acttagatac ctggtatgtg ggaatgaatg attttgcccg aattgccgga gggcagaata
     5101 gcagaagcaa tattctttct cccagagcat ttttggagtt tttggctaag atatttaccc
     5161 tgggttatgt ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata
     5221 ttaagtcctc atcttatatc aaaaataatg atggtagtga gataatgaag tttgttatga
     5281 ataatcctga aggggagcga gcggatttat ctaaggtgga aatagaaatt acacttgcct
     5341 cagcttttaa taatgggatt cgtgaaggac atacagtcat tatatttaca caacctgatg
     5401 gttcgactaa tcgttatgaa gggaagtcct ttgaaagaaa agatgagagt tcattacacc
     5461 tgattactaa caagattctg gcgtgttacc aacgcgaagc taacaaggaa atagctcgtc
     5521 tattaaatat tccgcaggag ttaaataatt ctcaggactt gaataactcg caggtgagtt
     5581 gtaaagattc agttgattct acgattacgg atttattaga aaaacca
//
LOCUS       DAZFRN010000245         5622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFRN010000245 DAZFRN010000000
VERSION     DAZFRN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.247"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(29..340)
                     /locus_tag="ACHKUB_004839"
     CDS             complement(29..340)
                     /locus_tag="ACHKUB_004839"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6657736.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            539..1237
                     /locus_tag="ACHKUB_004840"
     CDS             539..1237
                     /locus_tag="ACHKUB_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6657737.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1282..2181)
                     /gene="eamA"
                     /locus_tag="ACHKUB_004841"
     CDS             complement(1282..2181)
                     /gene="eamA"
                     /locus_tag="ACHKUB_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO6657738.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2376..3563
                     /gene="ydeE"
                     /locus_tag="ACHKUB_004842"
     CDS             2376..3563
                     /gene="ydeE"
                     /locus_tag="ACHKUB_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO6657739.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWIIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTNLPPSSLFIILTLVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3690..3785
                     /gene="mgtS"
                     /locus_tag="ACHKUB_004843"
     CDS             3690..3785
                     /gene="mgtS"
                     /locus_tag="ACHKUB_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO6657740.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3785..3889
                     /gene="mgtT"
                     /locus_tag="ACHKUB_004844"
     CDS             3785..3889
                     /gene="mgtT"
                     /locus_tag="ACHKUB_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO6657741.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(4004..4894)
                     /gene="dgcZ"
                     /locus_tag="ACHKUB_004845"
     CDS             complement(4004..4894)
                     /gene="dgcZ"
                     /locus_tag="ACHKUB_004845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO6657742.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5149..5541)
                     /gene="ydeI"
                     /locus_tag="ACHKUB_004846"
     CDS             complement(5149..5541)
                     /gene="ydeI"
                     /locus_tag="ACHKUB_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO6657743.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1543 a         1341 c         1168 g         1570 t
ORIGIN      
        1 caggtaatag tttaataata ttagattctt actcttgcag tagtttcatc gcaaattcaa
       61 gaactgcctg tccgttcatg gtgccgtagt ctttcatctc gataacggct acaggcattt
      121 tcccctgtgc aacggcttca atctctggtt tctgaaagcg aacttgcggg ccaagtaaaa
      181 caacgtcaac ttcgttattt ttaattttcc ctttagcctc tgcaatcgcc aatgcggaaa
      241 tattaacttc aagtgaaata gcgttagcat gatcaatcat acgtttaacc agcatactgg
      301 ttgacatgcc cgcagcgcaa actaacaata tttttttcat attttccctt actggtatat
      361 aacagattac attgtttaaa tatcatatcc cgcagtagct atacgtcctg gtacattgat
      421 cacagtaata cagtgataaa gttgttcaac atcagggtga ttattaaacg actcggccat
      481 acgataaacc aatcaggact caactccttg ttttcaatgg ctcttaaaag gtgcaaaagt
      541 gatttttcaa aagattgccc gcttacttaa atccgaaatc aatggcaatt catggcatgt
      601 tggtgatttg ttgccgtcag aagcggaact cgctgttcgt tataatgttt cgcgtaatac
      661 cctgcgtaag gcattatcct tgctggaagg cgaaggaatt attcacagaa agcatggttc
      721 aggaacatac attcaaaaaa agaattttgt tgcacacatt gatcacatga acagtttcag
      781 tgaaattgcg cataaaagcg gcaaagaggc agggagtcag attatgaaat ttgaagtgca
      841 ggatgcttca cccactattg cgactgagct gaatttagtg actggtgagc aggtttatta
      901 cataaagaga ctgcgattca ttgaggataa tgcagcacaa ttagaagaaa cgtggatgtc
      961 agtagcacgt tttcctgatt taaccgtgtc acatatgcaa aaatccaaat tttcatatat
     1021 tgagaatgaa tgcgggatca aaatcattgg cacctttgaa actttctccc ctacttttcc
     1081 taccccagaa atcgccagta ttttacggat cagcccacgg gatcccatac ttaaaattca
     1141 gacccaggct gtggacagta actctattcc gctggattat tcgttacttt acagcaatat
     1201 tttcgagttc caggtaaagt actttttccc gcgataaaag cagacgcaca gagtgctgac
     1261 atcacattct gtgcgtatgg tttagcctct aacctttacc gctttacgcc accgcaagcc
     1321 aaatacattg atatacagcc cggtcataat gagcaccgca cctaaaaatt gcagacccgt
     1381 taagcgttca tccaacaata gtgccgcact tgccagtccg actacgggca ccagtaacga
     1441 taacggtgca acccgccagg tttcatagcg tcccagtaac gtcccccaga tcccataacc
     1501 aacaattgtc gccacaaacg ccagatacat cagcgacaag atggtggtca tatcaatagt
     1561 aaccagactg tgaatcatgg atgcggaacc atcgagaatc agcgaggcaa caaagaaggg
     1621 aatgattggg attaaagcgc tccagattac cagcgacatc accgccggac gcgttgagtg
     1681 cgacatgatc tttttattga agatgttgcc acacgcccaa ctaaatgctg cagccagggt
     1741 caacataaag ccgagcatcg ccacatgctg accgttcaga ctatcttcga ttaacaccag
     1801 tacgccaaaa atcgctaagg cgatccccgc caattgtttg ccatgcaatc gctccccgaa
     1861 agtaaacgcg ccaagcacga tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag
     1921 tccagcaggc ataccgaagt taatggcaca aaaaagaaaa gcaaactgcg caaaactgat
     1981 ggttaatcca taccccagca gcaaattcag tggtactttc ggtcgtgcga caaaaaagat
     2041 agccggaaaa gcgaccagca taaagcgcaa accggccagc atcagcggtg gcatgttatg
     2101 aagccccact ttgatgacca caaaatttag cccccatacg accactacca gtagcgccaa
     2161 caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta agcaatcaac
     2221 ttagctgaat ttacttttct ttaacagttg attcgttagt cgccggttac gacggcatta
     2281 atgcgcaaat aagtcgctat acttcggatt tttgccatgc tatttcttta catctctaaa
     2341 acaaaacata acgaaacgca ctgccggaca gacaaatgaa cttatcccta cgacgctcta
     2401 ccagcgccct tcttgcctcg tcgttgttat taaccatcgg acgcggcgct acgctgccat
     2461 ttatgaccat ttacttgagt cgccagtaca gcctgagtgt cgatctaatc ggttatgcga
     2521 tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata
     2581 agttcgacaa gaaacgctat atgttactgg caattaccgc cttcgccagc ggttttattg
     2641 ccattccttt agtgaataac gtgacgctgg tggtgctctt ttttgccctt attaactgcg
     2701 cctattccgt ttttgctacc gtgctgaaag cctggtttgc cgacaatctt tcgtccacca
     2761 gcaaaacgaa aatcttctca atcaactaca ccatgctcaa cattggctgg atcatcggtc
     2821 cgccgctcgg cacgctgttg gtgatgcaga gcatcaatct gcccttttgg ctggcggcta
     2881 tctgttccgc gtttcccatg cttttcattc aaatttgggt aaagcgcagc gagaaaatca
     2941 tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat aaggcactgt
     3001 tgtggtttac ctgctctggc tttctggctt cttttgttag cggtgccttt gcctcgtgca
     3061 tttcacaata tgtgatggtg attgctgatg gggattttgc cgaaaaggtg gtcgcggttg
     3121 ttcttccggt taatgctgcc atggtagtta cgttgcaata ttccgtgggc cgccgcctta
     3181 acccggctaa catccgcgcg ctgatgacag caggcaccct ctgtttcgtc atcggtctgg
     3241 tcggttttat tttttccggc aacagcctgc tattgtgggg tatgtcagct gcggtattta
     3301 ctgtcggtga aatcatttat gcgccgggcg agtatatgtt gattgaccat attgcgccgc
     3361 caggaatgaa agccagctat ttttctgccc agtctttagg ctggcttggt gctgcaatta
     3421 acccattagt gagtggcgta gtgctaacca acctgccgcc ttcctcgctg tttatcatct
     3481 taacgttggt gattattgct gcgtgggtgc tgatgttaaa aggaattcgt gcaagaccgt
     3541 gggggcagcc cgcgctttgt tgatttaagt cgaacacaat aaagatttaa ttcagccttc
     3601 gtttaggtta cctctgctaa tatctttctc attgagataa aaattaaggt aagcgaggaa
     3661 acacaccaca ccataaacgg aggcaaataa tgctgggtaa tatgaatgtt tttatggccg
     3721 tactgggaat aattttattt tctggttttc tggccgcgta tttcagccac aaatgggatg
     3781 actaatgaac ggagataatc cctcacctaa ccggcccctt gttacagttg tgtacaaggg
     3841 gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac cggcggtgaa tgcttgcatg
     3901 gatagatttg tgttttgctt ttacgctaac aggcattttc ctgcactgat aacggatcgt
     3961 tgacacagta gcatcagttt tctcaatgaa tgttaaacgg agcttaaact cggttaatca
     4021 cattttgttc gtcaataaac atgcagcgat ttcttccggt ttgcttaccc tcatacattg
     4081 cccggtctgc tcttccaatg accacatcca gaggctcttc aggaaatgcg cgactcacac
     4141 ctgctgtcac ggtaatgttg atatgccctt cagaatgtgt gatggcatgg ttatcgacta
     4201 actggcaaat tctgacacct gcacgacatg cttcttcatc attagccgct ttgacaataa
     4261 tgataaattc ttcgcccccg tagcgataaa ccgtttcgta atcacgcgtc caactggcta
     4321 agtaagttgc cagggtgcgt aatactacat cgccgattaa atgcccgtag gtatcattaa
     4381 ccaatttaaa tcggtcaata tccaacaaca ttaaataaag attcagaggc tcagtgttgc
     4441 gtaactgatg atcaaaggat tcatcaagaa cccgacgccc cggcaatccc gtcaaaacat
     4501 ccatattgct acggatcgtc agcaaataaa ttttgtaatc ggttaatgcc gcagtaaaag
     4561 aaagcaaccc ctcctgaaag gcgtcgaaat gcgcgtcctg ccagtgattt tcaacaatag
     4621 ccagcattaa ttcccgacca cagttatgca tatgttgatg ggcagaatcc attagccgaa
     4681 cgtaaggtaa ttcatcgtta tcgagtggcc ccagatgatc aatccaccga ccaaactggc
     4741 acagtccata agaatggtta tccgttattt ctggcttact ggcatctctc gcgaccacgc
     4801 tgtgaaacat actcaccagc cactggtagt gggcatcgat agccttattg agatttaaca
     4861 agatggcatc aatttccgtt gtcttcttga tcattgccac tcctttttca cagttccttg
     4921 tgcgcgctat tctaacgaga gaaaagcaaa attacgtcaa tattttcata gaaatccgaa
     4981 gttatgagtc atctctgaga taacattgtg atttaaaaca aaatcagcgg ataaaaaagt
     5041 gtttaattct gtaaatcacc tctgcattat cgtaaataaa aggatgacaa atagcataat
     5101 ccaataccct aatggcccag tagttcaggc catcaggcta atttattttt atttctgcaa
     5161 atgagtgacc cgaacgacgg ccggcgcgct tttcttatcc agactgccgc taatgttgat
     5221 catctggtcc ggctgaactt ctcgtccatc aaagacggcc gcaggaataa cgacattaat
     5281 ttcaccgctc ttatcgcgaa aaacgtaacg gtcctctcct ttgtgagaaa tcaaattacc
     5341 gcgtagtgaa accgaagcgc catcgtgcat gttttttgcg aaatcaacgg tcattttttt
     5401 tgcatcatcg gttccgcgat agccatcttc tattgcatga ggcggcggtg gcgctgcatc
     5461 ctgttttaaa ccgccctggt catctgccaa cgcataaggc atgacaagaa aacttgctaa
     5521 tacaatggcc tgaaatttca tactaactcc ttaattgcgt ttggtttgac ttattaagtc
     5581 tggttgctat ttttataatt gccaaataag aatactgcca at
//
LOCUS       DAZFRN010000246         5600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFRN010000246 DAZFRN010000000
VERSION     DAZFRN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.248"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..244
                     /locus_tag="ACHKUB_004847"
     CDS             <1..244
                     /locus_tag="ACHKUB_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657744.1"
                     /translation="LENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGFALGYY
                     CHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(298..1803)
                     /gene="glpD"
                     /locus_tag="ACHKUB_004848"
     CDS             complement(298..1803)
                     /gene="glpD"
                     /locus_tag="ACHKUB_004848"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO6657745.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            1993..2319
                     /gene="glpE"
                     /locus_tag="ACHKUB_004849"
     CDS             1993..2319
                     /gene="glpE"
                     /locus_tag="ACHKUB_004849"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO6657746.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2364..3194
                     /gene="glpG"
                     /locus_tag="ACHKUB_004850"
     CDS             2364..3194
                     /gene="glpG"
                     /locus_tag="ACHKUB_004850"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO6657747.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3211..3969
                     /locus_tag="ACHKUB_004851"
     CDS             3211..3969
                     /locus_tag="ACHKUB_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO6657748.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(3951..5549)
                     /gene="rtcR"
                     /locus_tag="ACHKUB_004852"
     CDS             complement(3951..5549)
                     /gene="rtcR"
                     /locus_tag="ACHKUB_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO6657749.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGTEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1258 a         1482 c         1510 g         1350 t
ORIGIN      
        1 gctggaaaat atccgaaatt tctttctgac gtttgtctgg cttcccctga atttttcccc
       61 tttttggccg cagccttgga ctgattttgt cggcccgatt agtgcacagc ttggttttgc
      121 attgggatat tattgccatt ggtgcagaaa aaatagaaac cagaggaaga agtggggcga
      181 ttgggtaacg tgcttaagtt tggcgatttt agctcagggg ccgttattca attatttaca
      241 atgaatagtc taaggaatcc aaatagcccg gttgcacttc acaaccgggc atcatcctta
      301 cgacgccagc gacaacttct gctgcgtata ctccaccagc cactgactca cacgagattg
      361 ttgatccgca tttagccaca tgccttgttt tgtgcgacgc cacagggcgt cgtcggcgcg
      421 gcggacccat tcgtgatcca ccaggtattt cagctccgct tcgtagaact catgaccgaa
      481 atcttccccg agatcgctta ccgttcccgc attgccgagc agcagctcgc tgttgctgcc
      541 gtaagtgcga gcgtaatgac gcgccagcga ttcagtcagg aatggatagc gacggcgcag
      601 acgagctgcg taatcgtcgc ggtcgccttc aatggcacca cccggcagca cgctctcttt
      661 cgtccacgcc gggccaatac cctgataata cggcgtcagt ttttccagcg catgttctgc
      721 cagttttcgg taggtggtca gcttaccgcc gaataccgac agcagcggtg ctttgccgtt
      781 ttcatcatgg atatcaaggg tgtaatcacg ggtaatagcc tgcggcgagt cggactcatc
      841 atcacacagc ggacgcacac cggagtaggt ccagacgata tcgtcacggc taagctgttt
      901 tttaaagtgc gcgttataca ctttcagcag atagttgatt tcactctctt caatcttcac
      961 cgctttcgga tcgcctttat actctacgtc ggtggtgccg atgatggaaa attcgtccat
     1021 ccacgggatc acgaacacaa tacgtttatc ttcgttttgc agaatgtagg cctgcttctg
     1081 ggtatgcaca cgcggcacca caatatggct gcctttgatc aggcgaatgc cataaggcga
     1141 aggcagatgc atcccctcgt cgaagaactg tttcacccac gggccggtgg cgttaaccag
     1201 gccgcgcgct tgccagctat attttttgcc ggtatcgata tcttccgctt ccacaatcca
     1261 caggccgttt tcgcggcgag cagaggtggc gcgagtccga gtaagcactt cgccgccttt
     1321 acgcaccacc atctgggcgt tggcgagtac cagacgggcg tcgtctaccc aacagtcaga
     1381 atattcgaat ccgcgcttaa tttccggttt taacactgaa tttgcgccaa aacgcaaacc
     1441 agttgatccc ggcaggctgg tgcgtttacc cagatgatcg tacataaaca gaccaatgcg
     1501 aatcatccac gccgggcgca gatgcggacg atgtggcagg cgaaaacgca tcgggaaggc
     1561 gatatgcgga gccattttca gcagcacttc acgttcagcc agcgcctcgc tgaccaggcg
     1621 gaattcatag tgctcaaggt agcgcaggcc accgtgaatg agttttgaac tggcggaaga
     1681 ggtcgcgcaa gcgagatcct gcgcctccag catcagcacg gataaaccgc gtccagcggc
     1741 gtctgccgcg ataccagcac cattgatgcc gccccctatc acaatcagat ctttggtttc
     1801 catgctgccc tcattcactt tcgttaaagc tcataaatgt tcgttatcga acatattagc
     1861 aaagaatcgc gctttaggta acattgaaaa aacattttag agtgatatgt ataacattat
     1921 ggcgtttatc tgccgcttcg acgtaaactg tgcggtaaat ttgcccactt gtttgtaaag
     1981 aaagagagac gcatggatca gttcgaatgt attaacgttg ccgtcgcgca ccagaagttg
     2041 caggaaaaag aggcggtgct ggtcgatatt cgcgatccac agagtttcgc aatggggcat
     2101 gcggtgcagg ctttccattt aaccaacgac acgctgggcg cgtttatgcg tgataacgac
     2161 tttgacactc cggtgatggt gatgtgttat cacggcaata gcagcaaagg cgcggcgcag
     2221 tatctgctgc aacagggcta cgatgtggtc tatagcattg acggcggttt tgaagcctgg
     2281 cagcgtcagt ttcccgcaga ggtggcgtac ggcgcgtaac gctttatact gtcccctttt
     2341 gtgtggaata agcgacagca acgatgttga tgattacctc ttttgctaac ccccgcgtgg
     2401 cgcaggcgtt tgttgattac atggcgacgc agggtgttat cctcacgatt caacaacata
     2461 accaaagcga tgtctggctg gcggatgagt cccaggccga gcgcgtacgg gcggagctgg
     2521 cgcgttttct cgaaaacccg gcagatccgc gttatctggc ggccagctgg caggcaggcc
     2581 ataccggcag tggtctgcat tatcgccgtt atcctttctt tgctgccttg cgtgaacgcg
     2641 caggtccggt aacctgggtg gtgatgatcg cctgcgtggt ggtgtttatc gccatgcaaa
     2701 ttctcggcga tcaggaagtg atgttatggc tggcctggcc attcgatcca acactgaaat
     2761 ttgagttctg gcgttacttc acccacgcgt taatgcactt ctcgctgatg catatcctct
     2821 ttaacctgct ctggtggtgg tatctcggcg gtgcggtgga aaaacgcctc ggtagcggta
     2881 agctaattgt tattactctc attagcgccc tgttaagcgg ctatgtgcag caaaaattca
     2941 gcgggccgtg gtttggcggg ctttctggcg tggtgtatgc gctgatgggc tacgtctggc
     3001 tacgtggcga acgcgatccg caaagtggca tttacctgca acgtgggtta attatctttg
     3061 cgctgatctg gattgtcgcc ggatggtttg atttgtttgg gatgtcgatg gcgaacggag
     3121 cacacatcgc cgggttagcc gtgggtttag cgatggcttt tgttgattcg ctcaatgcgc
     3181 gaaaacgaaa ataattccag ggatttataa atgaaacaaa cacaacgtca caacggtatt
     3241 atcgaactgg ttaaacagca gggttatgtc agtactgaag agctggtaga gcatttctcc
     3301 gtcagcccgc agactattcg ccgcgacctc aatgagctgg cggagcaaaa cctgatcctg
     3361 cgtcaccatg gcggtgcggc gctgccttcc agttcggtta acacgccgtg gcacgatcgc
     3421 aaagccaccc agaccgaaga aaaagagagg attgcccgca aagtggcgga gcaaatcccc
     3481 aacggctcga cactgtttat cgatatcggc accacgccgg aagcggtggc gcacgcgctg
     3541 cttaatcaca gcaatttgcg tattgtcacc aacaatctca atgttgctaa cacgttgatg
     3601 gtaaaagaag atttccgcat tattctcgcc ggtggcgaat tacgcagccg cgatggcggg
     3661 atcattggcg aagcgacgct cgattttatc tcccagttcc gccttgattt cggcattctg
     3721 gggataagcg gcatcgatag cgacggctcg ctgctggagt tcgattacca cgaagttcgc
     3781 accaagcgcg ccattattga gaactcgcgc cacgttatgc tggttgtcga tcactcgaaa
     3841 tttggccgta acgcgatggt caatatgggc agcatcagca tggtagatgc cgtctacacc
     3901 gacgccccgc cgccagtaag cgtaatgcag gtgctgacgg accatcatat tcaactggag
     3961 ctgtgctgat cctgcacggc ttcccacgtc agaccaaaac gcgccaggta tttgcgtagc
     4021 cgatccgcgt cattgacgct ggctttgccc tggcgcgaaa catcaaaaag caggcgtccg
     4081 gcagcggaaa gcgactttgc ctggcgacag agagctatca cgtgttccag ttgcagcctg
     4141 tcaaaaaggt cgatgttttc tgcctctgtg cccagcaacg ccgtaagcgc ggaggggcgg
     4201 ctctcctgcc agttatagcg cagacggttt atctcatctt caaccacgtc cagagtgatg
     4261 cgtccgctag tggcaaaggt ggccatccgc gtgacgctgg cagaaagttc gcgaaagtta
     4321 ccgcgccatg ttgcctgggg agaggtcgca aaagccaacc aggcgcgccg cgcttcggtg
     4381 ttaaaacgca cgctgtcgcc agtgagtgtg gcgtggcgct ccacttcata atcaaggttc
     4441 ggttcaatat cttcctggcg ctggcgtaga cccggcaggg tgaaggtcca gagattgatc
     4501 cgcgcgtaca gatcttcgcg aaatttgcct tcggcaacca gctggcgcaa atcgcgcacc
     4561 gttccggcga taagctgaaa atcactgctc acctggcgat cgctgccaaa cgggtaaaag
     4621 gttttctctt caatggcttt tagcagcatt gcttgttcgt ctgcgcccag ttcgccaatc
     4681 tcatcaagaa acaacattcc gccgttggcg ctgcgtaata aaccttcacg agattcccgc
     4741 gccccggtaa acgcgccttt tacatgacca aacagcgtcg acatggcggt atcgccgcgc
     4801 agggtggcgc agttcacttc cacaaacgcg ccgctaaact gatgccgcgc ctgttttaac
     4861 tcgaagatgc gtcgcgccag aaatgacttg cccgcgccgg ttggaccgtt aagcagaatc
     4921 ggcgcgcggg atttgatcgc cactttttcg atctgctcaa tcatgcggtt gaagtggggg
     4981 ttacgcgtgg caatgccgga cttaagaaaa tcaagcgttt gctggcgttc ctcggcaaag
     5041 cggctggcga tggcgttata acggcttaaa tcgagatcga taatcgtcac ttcacctgcg
     5101 ccgcgcggct gttctttttt gcgcggaggt gaagattgta tcagccgggc gggcaggtaa
     5161 cgcgcttccg ccagcagaaa ccagcaaatc tgcgcgacgt gggtgccggt ggtgatgtga
     5221 attaaatagt cttctttttc tggctgaaac tcgtaaccac gggcgaaatc atgcaggcag
     5281 gcgtaaacct cttcgaaatc ccacgggtta tgcagttcaa tctcaacgct caccacttct
     5341 gtttctggcg aaacgctggc gatatcgcgt ttaagcgttt cgaatagcga gcgcgagcgg
     5401 gtgtcgtgca acaattccag ccgatcgatg actaacgatt cttgctggca taaacaaagc
     5461 gtcggacgcc atttcgacca gcgctgactg ccgcgtccgg cataatccag cacggtaccg
     5521 acaaagccaa aagccactgt tttacgcatc ttagatatcc ttataaaaga cgatatttca
     5581 ttatcggtga attggttttg
//
LOCUS       DAZFRN010000247         5592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFRN010000247 DAZFRN010000000
VERSION     DAZFRN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.249"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..737
                     /gene="lpfD-O113"
                     /locus_tag="ACHKUB_004853"
     CDS             <1..737
                     /gene="lpfD-O113"
                     /locus_tag="ACHKUB_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO6657750.1"
                     /translation="DVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSG
                     AKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSC
                     EINNLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYA
                     TEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAA
                     PASLTGARPAPGQFTATATITVEIVR"
     gene            985..2025
                     /gene="pstS"
                     /locus_tag="ACHKUB_004854"
     CDS             985..2025
                     /gene="pstS"
                     /locus_tag="ACHKUB_004854"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO6657751.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2112..3071
                     /gene="pstC"
                     /locus_tag="ACHKUB_004855"
     CDS             2112..3071
                     /gene="pstC"
                     /locus_tag="ACHKUB_004855"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO6657752.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3071..3961
                     /gene="pstA"
                     /locus_tag="ACHKUB_004856"
     CDS             3071..3961
                     /gene="pstA"
                     /locus_tag="ACHKUB_004856"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO6657753.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4052..4825
                     /gene="pstB"
                     /locus_tag="ACHKUB_004857"
     CDS             4052..4825
                     /gene="pstB"
                     /locus_tag="ACHKUB_004857"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO6657754.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4840..5565
                     /gene="phoU"
                     /locus_tag="ACHKUB_004858"
     CDS             4840..5565
                     /gene="phoU"
                     /locus_tag="ACHKUB_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO6657755.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1364 a         1348 c         1485 g         1395 t
ORIGIN      
        1 ttgatgttgg gattgctata gatattcttg gggttgggta tgtgaatgca ccttttgaat
       61 atcatgctaa caacccgtct ggtaatacaa agtacaattg caaccgcatt gaacctttaa
      121 gtatatctag tggggctaaa gcgatagtat atttttatat caagaagaca tttgcaggaa
      181 aattgattat tcctgaaaca aagatagtga cattgtatgg aacaattagc cgtgacaccc
      241 cggtggatta ctcacagccg atggctgatg tttatattcg gggcgatatt acagctccgc
      301 aaagttgcga aataaataat ttacagccag tttattttga ttttaaagaa ataccagctg
      361 cagatttttc atctgttgtt ggaagtgcgg taacaacaca taaaattact aaaacggtca
      421 ctattgagtg tgaaaacctg gggatactga ataccgatga tatcagtacg tctttttatg
      481 ctaccgaacc caatacggac aattcaatgg tcgtaacttc aaactctaac gtggggataa
      541 aaatttacga caaaaataat aaggaaatca aagtgaacgg tggtgagttg ccaacagaca
      601 tgggtaaatc aacagtgtat ggtgaaaaat caggtagtgt aactttttca gctgctcctg
      661 cgagccttac tggagctcgc ccagccccgg ggcaatttac tgcaacagcg acgataactg
      721 ttgagattgt gcgctaatag gtctgacaac gattgttcat cacatcgttg tcatctcacc
      781 catttcttta tcacttttaa atgcatcatg acaaaaagat gacatgtggg attttcatct
      841 tcacatttaa aatcagtaag ttatttggta attcctttct gtcataaaaa tgtcatattc
      901 cttacatata actgtcacct gtttgtccta ttttgcttct cgtagccaac aaacaatgct
      961 ttatgaatcc tcccaggaga cattatgaaa gttatgcgta ccaccgtcgc aactgttgtc
     1021 gccgcgacct tatcgatgag cgctttctct gtgtttgcag aagcaagcct gacaggtgca
     1081 ggtgcaacct tccctgcgcc ggtgtatgcc aaatgggctg acacttacca gaaagaaacc
     1141 ggtaataaag ttaactacca gggtatcggt tcttccggtg gcgtaaaaca gattatcgct
     1201 aataccgttg attttggtgc ctctgacgcg ccgctgtctg acgaaaaact ggctcaggaa
     1261 ggtctgttcc agttcccgac cgtgattggc ggcgtggtgc tggcggttaa cattccaggg
     1321 ctgaagtctg gcgaactggt gctggatggt aaaaccctcg gcgacatcta cctgggcaaa
     1381 atcaagaagt gggatgatga agccatcgcc aaactgaatc cgggtctgaa actgccttca
     1441 caaaacattg ctgtagtacg ccgcgcagat ggctccggga cttccttcgt cttcaccagc
     1501 tacctggcga aagtgaacga agagtggaaa aacaacgttg gtactggctc taccgtaaaa
     1561 tggccgatcg gtctgggcgg taaaggtaac gacggtatcg ccgcgttcgt tcagcgtctg
     1621 ccgggtgcaa ttggttatgt tgaatatgct tacgcgaagc agaacaacct ggcgtacacc
     1681 aaactgatct ccgctgatgg taaaccggtt agtccgaccg aagaaaactt cgctaatgca
     1741 gcaaaaggtg cagactggag caaaaccttc gctcaggatc tgaccaacca gaaaggcgaa
     1801 gatgcatggc ctattacctc taccacgttc attctgatcc acaaagatca gaagaaacca
     1861 gaacaaggca cagaagtgct gaaattcttc gactgggcgt acaaaaccgg ggctaaacag
     1921 gcgaacgacc tggattacgc cagcctgccg gatagtgtag ttgaacaggt tcgcgctgcg
     1981 tggaagacca atattaaaga cagtagcggt aagccgctgt actaataaaa ctccaggccg
     2041 ggtacggtgt tttacgccgc atccggcatt acaaaatgac tttgtaaacg cgtttaactg
     2101 aagagtaact tatggctgca accaagcctg cttttaaccc accgggtaaa aagggcgaca
     2161 taattttcag cgtgctggta aaactggcgg cgctgattgt gctattgatg ttgggtggca
     2221 ttattgtctc tctgatcatc tcctcctggc cgagcattca gaaatttggt ctggctttcc
     2281 tatggaccaa agagtgggat gcaccgaacg atatctacgg ggcgctggtg ccgatctacg
     2341 gtacgttggt gacttcgttt atcgcgctgc tgatcgccgt cccggtgagt ttcggtatcg
     2401 ccctgttcct gactgagctt gcgcctggct ggctgaaacg cccgctgggt atcgccattg
     2461 agctgctggc agccattcca agtatcgttt acggcatgtg gggcctgttt atctttgcgc
     2521 cgctgttcgc cgtttacttt caggagccgg tcggcaatat catgtcgaat atcccgattg
     2581 ttggcgcgct gttctctggc cccgcatttg gtatcggtat cctcgcggca ggcgtgatcc
     2641 tcgccatcat gattattccg tacattgcgg cggtaatgcg tgatgtgttc gaacaaaccc
     2701 cggtgatgat gaaagagtcg gcctacggta ttggctgcac cacctgggaa gttatctggc
     2761 gtatcgttct tccgttcacc aaaaatggtg ttatcggcgg catcatgctg gggctgggcc
     2821 gcgcgctcgg tgaaaccatg gcggtgacct ttatcatcgg taacacctac cagctcgaca
     2881 gcgcctcgct gtatatgccg ggcaacagta tcacctctgc gctggcgaac gaatttgcgg
     2941 aagcggaatc cggtctgcac gttgccgcac tgatggaact gggcctgatc ctgtttgtga
     3001 ttaccttcat cgtcctcgcc gcatcgaagt ttatgattat gcgcctggct aagaatgagg
     3061 gggcacgcta atggctatgg ttgaaatgca aaccactgcg gcgctggctg aatctcgccg
     3121 caaaatgcag gcgcgtcgcc gcctcaaaaa ccgtattgcg ctgacgctct cgatggcgac
     3181 gatggccttc ggcctgttct ggctgatctg gattttaatg tccaccatca ctcgcggtat
     3241 cgacggtatg tcgctggcgc tgttcactga aatgacgccg ccgcccaata cggaaggtgg
     3301 tggtctggcg aacgccctgg cgggtagcgg gctgttaatt ttgtgggcca cggtattcgg
     3361 tacgccgctg ggcattatgg cggggattta tctggcggaa tatggtcgta aatcctggct
     3421 ggcagaagtg attcgcttca ttaacgacat tctgctctcc gcgccgtcga ttgtggttgg
     3481 tctgtttgtt tacaccatcg tggtggcgca gatggagcac ttctctggct gggcgggtgt
     3541 aattgcgctg gcgctgttgc aggtgccgat tgttatccgc accaccgaga acatgctgaa
     3601 actggtgccg gacagcctgc gtgaagcggc ttatgcgctg ggtacaccga agtggaaaat
     3661 gatctccgcg attacgctga aagcgtcggt gtccgggatc atgaccggta tcctgctggc
     3721 gattgcccgt attgccggtg aaaccgcgcc gctgctgttt accgcgctct ccaaccagtt
     3781 ctggagcacg gacatgatgc agccgatcgc caacctgccg gtgacgatct ttaagtttgc
     3841 gatgagcccg tttgcagaat ggcagcaact ggcctgggcc ggggtgttga ttattaccct
     3901 gtgcgtactg ctgctgaaca ttctggcgcg cgttgttttt gcgaagaata aacacggtta
     3961 acactttgcc ggatgcggcg tgaacgcctt atccggccta cggtaagcct gattcgcgaa
     4021 gtgcatcagg cacgatgagg aaaagattgc aatgagtatg gttgaaactg ccccgagtaa
     4081 aattcaggtt cgtaatttga acttctacta cggcaaattc catgccctga aaaacatcaa
     4141 cctggatatc gctaaaaacc aggttacggc gtttatcggg ccgtccggct gcggtaaatc
     4201 gacgctgctg cgtaccttca acaaaatgtt tgaactgtac ccggagcagc gtgcggaagg
     4261 tgaaattctg cttgatggcg acaacatcct gaccaactct caggatatcg ccctgctgcg
     4321 tgcgaaagtg ggcatggtgt tccagaaacc gacgccgttc ccgatgtcca tctacgacaa
     4381 catcgccttt ggcgttcgtc tgtttgagaa gctctcccgt gccgacatgg atgagcgcgt
     4441 gcagtgggca ttgaccaaag ccgcattgtg gaacgaaacc aaagataaat tgcaccagag
     4501 cggttactct ctctctggtg gtcagcaaca gcgtctgtgt attgcgcgtg gtatcgccat
     4561 tcgcccggaa gtgctgctgc tcgacgaacc gtgttcggcg ctcgacccca tctctaccgg
     4621 gcgtattgaa gagctgatca ccgaactgaa gcaggattac accgtggtga tcgtcaccca
     4681 caacatgcag caggctgcgc gttgttccga ccacacggcg tttatgtacc tgggcgaatt
     4741 gattgagttc agcaacacgg acgatctgtt caccaagcca gcgaagaaac aaacagaaga
     4801 ctacatcacc ggtcgttacg gttgattcag gagtgcgtta tggacagtct caatcttaat
     4861 aaacatattt ccggccagtt caacgccgaa ctggaaagta tccgcacgca ggtgatgacc
     4921 atgggcggca tggtggagca gcagctttct gatgcaatca ccgcgatgca taaccaggac
     4981 agcgatctgg cgaagcgcgt gatcgaaggc gacaagaacg tcaacatgat ggaagtggcg
     5041 atcgatgaag cctgcgtgcg cattattgcc aaacgtcagc cgacggcgag cgacctgcgt
     5101 ctggttatgg tgatcagtaa aaccattgcc gagctggagc gtattggtga cgtggcggac
     5161 aaaatctgcc gtactgcgct ggagaaattc tcccagcagc atcagccgtt gctggtaagt
     5221 ctggagtcgc tgggccgtca taccatccag atgctgcacg acgtgctgga cgcgttcgcg
     5281 cggatggaca ttgacgaagc ggtacgtatt tatcgtgaag ataaaaaagt cgatcaggaa
     5341 tacgaaggta ttgttcgtca actgatgacc tacatgatgg aagattcgcg taccattccg
     5401 agcgtactta ctgcgctgtt ctgcgcgcgt tctatcgaac gtattggcga ccgctgccag
     5461 aatatttgtg agtttatctt ctactacgtg aaggggcagg atttccgtca tgtaggcggt
     5521 gatgagctgg ataaactgct ggcgggaaaa gatagcgaca aataattcac cagacaaatc
     5581 ccaataactt aa
//
LOCUS       DAZFRN010000248         5555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFRN010000248 DAZFRN010000000
VERSION     DAZFRN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.250"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>5555
                     /gene="cdiA"
                     /locus_tag="ACHKUB_004859"
     CDS             <1..>5555
                     /gene="cdiA"
                     /locus_tag="ACHKUB_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFO6657756.1"
                     /translation="APELTSSGVLVASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQ
                     QNGTLYSAADLTLDIPDIRNSGLITGDNGLTLNTASLSNPGKITADTLNVRATTLDGD
                     GLLQGAGALALEGDTLSQGCNGRWLTAGDLTLRGKTLNTAGTTQGQNLTVQADNWANS
                     GSVLATGNLTASATGQLTSTGDIMSQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNS
                     GTVQGNHVTLHHSNTDNSGTVTGLSGLTLHSADGLTNSGALLSQNSLTLSAGDVTNSG
                     RIQGRNITLDASSLTSSGAVQSALDLALTLSGDVIAATGSKITALGDARLSGKVLGNQ
                     GLISAKTLEVNGDSLSNGGEISGVNDLNVTLSGNLQQHGKMLTDGALNVNARDISNSG
                     QLQGADNRITASSLTNSGRVQGDSGLTLTLLNALTNQASGVLLSQNVSALSAPVLTND
                     GTIQGNGKTTLSAATQAHNSGKILSGGELTFTTPDYSGSGWLQATDLLLNVAKLAGNG
                     TVMAANQATLTGSSLTNRGLFQAAQLNVNTQIITNSGTLLGNQGLTIKGNSLNNAGGK
                     VFSGGDMLADMVSLSGAGQLVALGNLTLKLTRGLIAQGVIAANKELSVSSQGDITNGA
                     TLQGNGITLNAAGRLTNNGQLIAGNGTTALSGSGIAMNASGSLQAGGEVSLTSRGDIT
                     LDAFTGTTGSLMLTAAGSVINTSLLYAGNNLSLFASTIRNHHGDMLAGDSLVMQKDVS
                     GAANAEVINTSGNIETTRGDITIRTGHLLNQREGINETKSYIPVENVAVPDGANSVSV
                     RVGDLGEDGWGYYVKSWSGTAGGGFEAWAVPTEKGATRKFLTGTTRVDVGATGGDARI
                     SAGNNLLIDADKLDNTGSHLLASEFVSLSGSQLNNQSFFGYTQDEYNVYRYYGKLAMI
                     PNDGHLQYGDASADDRVTFTLSGAPEYVTRDTGQALRAVIQAGKNVTAVFSSDISNTS
                     TTSNAGRITNTLAAPEINTPAEKNISPGMAQLAPDGTEALTVTAPDWTDTITRLTIGS
                     GTDLASGIVEGNYPLPSGNNGYFVPSADPDSPYLITVNPKLDGLGKVDSSLFAGLYDL
                     LRMHPGQAPRETDPAYTDEKQFPGSSYFLDRLGLKPEKDYRFLGDAAFDTRYVSNYML
                     NQIGGRYINGVGSDTDQMRYLMDNAARAQKALGLKFGVALTADQVAALDQSILWYKAV
                     TIKGQTVMVPEVYLSPKDVTLQNGSIISGQNVHLAGGNVTNSGSTLMAQNDLTIDSAD
                     SLGNLESGLINAGGALGLKAMGDINNISATITGKTVRLESLAGNVNNLTRYSHWQLDA
                     PEDSLALKHTYTGSIASVSAMDSLDIRADKNISVTGAEISAGGRAALIAGNDLSLNAI
                     DRVSSSRHANSESHQRSAELTTITAGDSVMLSAGRDVSSQGAGIAAEENITVRAGRDV
                     NLLAEESVTGSSSYSKKKTVIDETVRQQGTEIASGGDTTVNAGRDITAVASSVTATGN
                     ISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKTTHTISEDSASREAGSLLSGNRV
                     TVNAGDNLTVEGSDVVADRDVSLAAGNHVDVLAAINTDTSWRFKETKKSGLMGTGGIG
                     FTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSITAGKQAHISGSDVIANRDISITG
                     DSVVVDPGHDRRTVDEKFEQKKSGLTVALSGTVGSAINNAVTSAQETKESSDSRLKAL
                     QATKTALSGVQAGQAAAMASATGDPNATGVSLSLTTQKSESQQHSESDTVSGSTLNAG
                     NNLSVVATGKNRGDNRGDIVIAGSQLKAGGNTSLDAANDILLSGAANTQKTTGRNSSS
                     GGGVGVSIG"
BASE COUNT         1369 a         1476 c         1642 g         1068 t
ORIGIN      
        1 tgcgccggaa ctgaccagca gcggcgtact tgttgcttcc gccctgaata cacagtcaca
       61 gaccctgacc aacagcggtc tgttgcaggg ggaggcctca ctcaccgtta acacacagag
      121 gcttgataat cagcagaacg gcacgctgta cagtgctgca gacctgacgc tggatatacc
      181 ggacatccgc aacagcgggc ttatcaccgg tgataatggt ttaacgttaa acactgcctc
      241 cctcagcaat ccgggtaaaa tcaccgctga cacgctgaac gtcagggcga ccacgctgga
      301 tggtgatggt ctgttgcagg gcgccggtgc actggcgctt gaaggcgaca ccctctcaca
      361 gggctgtaac ggacgctggc tgacggcggg cgacctcacc ctccggggta aaacactgaa
      421 taccgcaggg accacgcagg gacagaatct caccgtgcag gcggacaact gggcgaacag
      481 tggttccgtg ctggcaaccg gtaaccttac tgcttcggca accggtcagt tgaccagtac
      541 cggcgatatc atgagccagg gggacaccac gctgaacgca gccaccacgg acaaccgggg
      601 cagtctgctt tcggccggca cgctctccct tgatggaaac tcactggata acagcggcac
      661 tgtccagggt aaccatgtca cgcttcatca cagcaacact gacaacagcg gtacggtcac
      721 ggggctgagc ggtctgaccc tgcatagcgc ggacgggctt accaacagtg gcgcgctgct
      781 gagccagaac tcgctgacgc tgagcgcagg agatgtgacc aacagcggcc ggattcaggg
      841 gcggaacatc actctggatg ccagcagcct gaccagcagc ggtgctgttc agagtgcgct
      901 ggatttggct ctgaccctga gtggcgatgt tattgccgcc accggcagca aaattaccgc
      961 cctgggcgat gcccgtctga gcggcaaagt gctgggtaac cagggattaa tcagcgcgaa
     1021 gacgctggaa gtgaatggcg attcgctcag caacggtggc gaaatcagtg gggttaacga
     1081 cctgaacgtg acactcagtg gtaatctgca acagcacggt aaaatgctga cggacggtgc
     1141 attaaacgtc aacgccagag acatcagcaa tagtgggcag cttcaggggg cggataaccg
     1201 aatcaccgcc agttcactga ccaactcagg tcgcgtgcag ggcgacagcg ggctgactct
     1261 tacattgctg aatgccctga ccaaccaggc cagtggtgtg cttctcagcc agaacgtatc
     1321 agcgctttcc gcacccgtgc tgaccaatga cggcaccatt cagggcaacg gtaaaaccac
     1381 gctcagcgcg gcaacgcagg cccacaacag tggcaagatt ctttccggcg gtgagctgac
     1441 gttcaccacg ccggactaca gtggcagtgg ctggctacag gccactgacc tgctgctgaa
     1501 cgtggcgaag cttgccggta atggcactgt gatggcggcg aatcaggcaa cgctcacagg
     1561 tagcagtctg accaaccgtg gcctgttcca ggccgcacaa ctgaacgtca acacgcagat
     1621 catcaccaac agcggcacgc tgctgggtaa tcagggcctg acaataaaag ggaacagcct
     1681 gaacaacgcc ggcgggaagg tattcagcgg cggtgatatg cttgctgata tggtctcgct
     1741 cagcggcgcg ggccagctgg ttgcactcgg taacctgacg ctgaagctca cacgtggttt
     1801 gatcgctcag ggcgttatcg ccgcgaataa agaactctcc gtcagcagcc agggtgatat
     1861 taccaatggc gccacattgc agggcaacgg tatcacgctg aatgctgcag gcaggctgac
     1921 caacaatggc cagttgatcg ccggcaacgg caccacggca ctgagcggca gcgggattgc
     1981 gatgaatgcc agcggcagcc tgcaggctgg cggggaagtc agcctgacca gcaggggaga
     2041 tatcactctc gacgctttta ccggcacgac gggtagtctg atgttaacgg ccgccgggtc
     2101 agtcattaac acctcactgt tgtatgcggg caataacctc tcgctgtttg ccagcaccat
     2161 ccggaaccat cacggcgata tgctggcggg tgacagcctg gtgatgcaga aagatgtcag
     2221 tggtgccgcg aatgctgaag tgattaatac ctccgggaat atcgaaacca cccgcggtga
     2281 tatcaccatc agaaccggac atttactgaa tcagcgggag ggtataaatg agacaaaatc
     2341 atacattccg gttgagaatg tcgcagtacc tgatggtgca aattctgttt cagtcagagt
     2401 gggcgattta ggtgaagatg gatggggata ttatgtgaag tcctggagcg gtactgccgg
     2461 tggtggtttt gaggcatggg ccgttccgac tgaaaaggga gccacccgga aatttctgac
     2521 tggcacaaca cgcgtcgatg ttggtgcaac agggggggat gccagaattt cggccgggaa
     2581 taatctgctg attgacgcgg acaaactgga taatacggga agtcatcttc ttgccagcga
     2641 gtttgtcagc ctgtcgggtt cacaactgaa taatcagtcc ttctttggct atacacagga
     2701 tgaatacaat gtgtatcgct attacggcaa actggccatg atccccaacg acgggcatct
     2761 gcagtatgga gatgcttctg ccgatgatcg cgttaccttt actctctccg gagctccgga
     2821 atatgtcacc agagacaccg gtcaggctct gagagccgtg attcaggcag gcaaaaatgt
     2881 cactgccgtt ttttccagtg atatcagtaa tacatccacc acctcaaatg caggacggat
     2941 cacaaatacc cttgcggcac ctgaaattaa cacgccggca gagaaaaata tcagtccggg
     3001 aatggctcaa ctggcgccgg atggtacaga ggcgctgacc gtcacagccc cggactggac
     3061 tgacaccatt acccgtctga cgatcggcag tggtacggac ctggcttccg gcatcgtgga
     3121 ggggaactat cccctgcctt ccggtaacaa tggttatttt gtcccgtcag cagacccgga
     3181 cagtccgtat ctgattacgg tgaatccgaa actggatggt ctcggaaagg tggacagcag
     3241 tctgtttgcc ggactgtatg acctgctcag gatgcacccc gggcaggcac cacgtgaaac
     3301 agacccggcg tatactgatg aaaaacaatt cccgggctca tcgtattttc ttgaccggct
     3361 cgggctgaaa ccggaaaaag attatcgctt cctgggggat gccgcttttg atacccgcta
     3421 tgtatcgaac tatatgctga accagatcgg ggggcgctat atcaacgggg ttggttcaga
     3481 caccgaccag atgcgctacc tgatggataa cgctgcccgt gcacagaagg cgctcggtct
     3541 gaagtttggt gtggcactga cagcagacca ggtggcggct ctcgaccaaa gtattctgtg
     3601 gtataaagcc gtcacgataa agggtcagac ggtgatggtg ccggaagtgt atctgtcacc
     3661 gaaagatgtg acgctgcaga acggcagtat tatcagcggt cagaatgtgc acctggcagg
     3721 agggaatgtc actaacagtg gcagtaccct gatggcgcag aatgatctca ccatcgacag
     3781 cgctgacagt ctgggtaacc ttgagtccgg gcttataaat gccggtggcg ctctcggact
     3841 gaaagcgatg ggcgatatca acaatatcag tgccacgatt accggtaaga ctgtccggct
     3901 ggagagtctc gccggcaatg tcaataatct cacccggtac tcacactggc agcttgatgc
     3961 accggaagac agcctcgccc tgaaacacac ttatacggga agcattgcct ctgtcagtgc
     4021 catggatagt ctggacatcc gggcagataa aaacatttcg gtcacagggg cagaaatcag
     4081 cgccggaggt cgcgcagcac ttattgccgg caatgatctc agtctgaatg ccattgacag
     4141 ggtaagcagc agtcgccatg caaacagtga atctcaccag cgcagtgcag aacttacgac
     4201 cattactgcc ggcgacagtg tgatgctgag tgcaggccgg gatgtgagca gtcagggtgc
     4261 aggtatcgca gcggaagaga acatcactgt ccgggccggg cgcgacgtta atctgctggc
     4321 ggaagagtcc gtgaccggca gcagttcata ctcgaaaaag aaaacggtca ttgatgaaac
     4381 cgttcgccag cagggaacgg aaatcgccag cggtggtgac accaccgtca acgcaggacg
     4441 ggatatcacc gctgttgcgt catccgttac cgcaaccggc aatatcagcg tgaatgccgg
     4501 tcgtgatgtt gccctgacca cggcgacaga aagtgactat cactatctgg aaacgaagaa
     4561 aaaaagcgga ggttttctca gtaagaaaac cacccacacc atcagtgagg acagtgcctc
     4621 ccgtgaagca ggctccctgc tgtcggggaa ccgcgtgacc gttaacgccg gtgataacct
     4681 gacggtagag ggttcggatg tggtggctga ccgggatgtg tcactggcgg cgggtaacca
     4741 tgttgatgtt cttgctgcca tcaatacaga tacgtcctgg cgctttaagg aaacgaagaa
     4801 atccggtctg atgggtaccg gcggtattgg tttcaccatt ggcagcagta agacaacgca
     4861 cgaccgccgc gaggccggga caacgcagag tcagagtgcc agcaccatcg gctccactgc
     4921 cggtaatgtc agtattaccg cgggcaaaca ggctcatatc agcggttcgg atgtgattgc
     4981 gaaccgggat atcagcatta ccggtgacag tgtagtggtt gacccggggc atgaccgtcg
     5041 tactgtggac gaaaaatttg agcagaagaa aagcgggctg acggttgccc tttccggcac
     5101 ggtgggcagt gccatcaata atgcggtcac cagtgcacag gagacgaagg agagcagtga
     5161 cagtcgtctg aaagccctgc aggccacaaa gacagcgctg tctggtgtgc aggccggaca
     5221 ggctgcggca atggcctccg caaccggtga cccgaatgcg acgggagtca gcctgtcgct
     5281 caccacccag aaatcggaat cacaacaaca ttctgaaagt gacacagtat ccggcagtac
     5341 gctgaatgcc gggaataatc tgtctgttgt cgcaaccggc aaaaacaggg gcgataaccg
     5401 cggagatatt gtgattgccg gaagccagct taaggccggt ggtaacacaa gcctggatgc
     5461 cgcgaatgat attctgttga gtggcgccgc aaacacacaa aaaacaacgg gcaggaacag
     5521 cagcagtggc ggtggcgtgg gtgtcagtat cggtg
//
LOCUS       DAZFRN010000249         5513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFRN010000249 DAZFRN010000000
VERSION     DAZFRN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.251"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..1936)
                     /gene="ehxB"
                     /locus_tag="ACHKUB_004860"
                     /gene_synonym="hlyB"
     CDS             complement(<1..1936)
                     /gene="ehxB"
                     /locus_tag="ACHKUB_004860"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO6657757.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIM"
     gene            complement(1986..4982)
                     /gene="ehxA"
                     /locus_tag="ACHKUB_004861"
     CDS             complement(1986..4982)
                     /gene="ehxA"
                     /locus_tag="ACHKUB_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO6657758.1"
                     /translation="MTVNKIKNIFNNATSTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADDLGIEIHRTERDDTAIANQFFGTAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PNLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSNLIQFVEKDNKHYQARIISELGDKDDIVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDRDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKADKIPHNNNERSGYIKASNIASEKNMVNITSVADDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            complement(4984..5499)
                     /gene="ehxC"
                     /locus_tag="ACHKUB_004862"
                     /gene_synonym="hlyC"
     CDS             complement(4984..5499)
                     /gene="ehxC"
                     /locus_tag="ACHKUB_004862"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO6657759.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWLSGERKWFIDWIAPFGHNIEL
                     YKHMRKKYPYELFRAIRLDEYSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
BASE COUNT         1645 a         1252 c          875 g         1741 t
ORIGIN      
        1 tcattattat attttccgac tcataatcaa gagcactggt tgcttcatca aaaataagaa
       61 ttcgaggatt tgtaacaaga gcccgggcta ttgcgatccg ttggcgctgc cctcctgaaa
      121 gaccaacgcc ctgttctcca acaatagtat tgtacccttc ttttagttct gtaataaaat
      181 catgggcacc tgcaagtctg gctgcctcaa tagcctgttc catagataca gccggagaag
      241 ctaatgtaat attatcgata atactacgat ttaatagtat attttcctgt aatacaacac
      301 caatctggcg tcgtaaccat tctggatctg caagtgataa atcatgccca tcaattaaaa
      361 tctgtccggt ctctggtata taaaagcgct gaagcagttt tgttaatgta ctttttcctg
      421 aaccagaacg gccaactata ccgataacat ccccctttga tatgtataaa ttaatattat
      481 tcaaaataac attaccgtca gaagaatacc gaaatctgac atttttaaat tcgatatccc
      541 cctgaatttc cggcagtata tttctgcctg actttttctc taccggtgta tttaatacat
      601 ccccaaggcg ctcaacagaa ataccaactt gctgaaaatc ctgccaaaga tgagccagtc
      661 tgataacagg agcaatgacc tgacctgcaa gcatattaaa cgcgattaac tgcccgatgc
      721 ttatctctcc cgatatgaca agatgtgctc ccatccatag actaattacc attacacttt
      781 tctgtatcag ctgtatcccc tgctgccctg tcattgcaac cagatttacc ctgaaactgg
      841 aagcaacata cccggccagt tgtttatccc atgtagcaat catctgtggc gatactgcca
      901 tggatttaat tgtatttatt gctgttaccg tttcgacaag aaaagcttga ttttctgcat
      961 tcctgagaaa cttatcatca agacgtcgac gtaataaggg tgatataaac aatgaccata
     1021 taacataaca aggtagtgac aataatataa ccagtgttaa ttgaggactg taataccaca
     1081 tgacacaaaa aaatattaca gaaaaaaaca gatcaagaac tgatgtcaac gcttgtccgg
     1141 ttaaaaaatt acgaatttgc tccagttccc ttactctggc aacggtctct ccgaccctgc
     1201 gattttcaaa atatgaaaca ggcaatgcaa gcaaatgtct gaataactta gcacccagtt
     1261 cgacgtcaat acgacttgta gtatgagaga aaatataagt tctggctccg gttaatatca
     1321 cttcaaaaag tatcactata ataaatgcta ttgtgataat atttaacgtt gaaaaccccc
     1381 ggtgaactaa aaccttatcc attacaacct gaaaaaaaag aggtgttatt aacgcaagaa
     1441 actgaataaa agcagaaaca gttaacacct caagtaaaat cctcctgtat ttcacaacag
     1501 aggggataaa ccaggaaaaa tcaaatttag ctaactctcc gactactgtt gctcttgacg
     1561 taaccagaat gacttttccc tgatacagtt tttcgaactc atcccgacta aaagttagtg
     1621 actgatgctg ttctggatca taaacaagat agcgtgatga atctttagta acacgagata
     1681 atatataatg cttaccgtca tcccgccata tcaacgcagg taaagaaatt attgacaatc
     1741 tggaaaaatg tttttctaca tattttgctt ttaagccaat ggatttcgct gccagtaacc
     1801 attcagtcac cccaaaatct tgtgtgtggg tattatactg atgccttata gtttcagcat
     1861 tgacagttat attatgatat tgtgcaagca atatcagtgc atacagacta ttatgagaac
     1921 tacatttact cattatataa cgatgaccat tcctcctgga atggccatca cctccgcttt
     1981 tagtctcaga cagttgtcgt taaagttgtt gaatgtgtgt tgttttgatg taaggataaa
     2041 ttatataaag atgctaatcg ttcatcacct gaatcgaacc ctgaaactga agaaataatc
     2101 ttgttaatat catcggcaac actggtgata ttaaccatgt ttttttcaga tgctatatta
     2161 ctggctttta tataacctga ccgttcatta ttattatgag gtatcttatc agcttttatc
     2221 tcacgaccat ctttatctgt tataacctca acaagatgat tatctgctcc tgaggcatct
     2281 ttcgcaaacc agtttttaaa tgttatccca ttgacatcat ttgactcatt aaatgaaagt
     2341 acaccattaa tggctttatt catgataaga tcatttccaa ctctcttaaa tgcgatatca
     2401 tcaaagctaa tatcagataa gtgcagacga tctcctttac ctccttcatc tgcaattgta
     2461 tgatgaccat agttttgcct ataaacataa atatcattac caaaaccacc attcagatag
     2521 tcattacctt cccctccatc cagaaggtca ataccaccac caccataaag tttatcatca
     2581 ccagtaccac ctgacagaat attataagct ccgtgtgcct gaagctcatc attaccgctt
     2641 cctcctgtca gataattatt accatcccct ccactcaatt tatcgtttcc actaccacca
     2701 aataactggt cgtctccctg tcctccggat atataatcat ccctatcatc gccgtatagt
     2761 cgatcattac cataattacc ttcgatataa tcgttcccat ctgcgccatg gaatatatca
     2821 ttaaacttac cgcctttgaa ttcatcatca tgttttccgc caatgagttc ttcaacagaa
     2881 tgaagattat ctatcacatc ataaggaatt ccaccggttc tgaattcaaa atcacgatat
     2941 tgtattttct ctgttcgctt ccctactgac acctcctgtt ccttaacgac ttcctgcaat
     3001 accttcacgt caccatacat attttttgaa actatatatt caccaggttt tgatgctcct
     3061 gttgcatcaa ttgttagttt accaacatcc gtcttattat aagatacggt atcatgacct
     3121 tcaccagcaa atacttctga tgagcctgcc ccagaataaa ctatatcgtc tttatctccg
     3181 agctcagaaa ttattctcgc ctgatagtgt ttgttatcct tttcaacaaa ctgaatcaaa
     3241 tttgaataat catatacacc tttatgattt tttatgcctt ttacagacca tgtatcctta
     3301 ccatttacaa taagagatgt catatattca tatttaccac tctgttttct ttctctgact
     3361 tcttctcctg gggtaaatgt tggtgttaca aatgtcaaaa cacttgatac attacctgtt
     3421 gaaaggtcta tagtcccttt ttgaggatca aaaacttgtt gtgtaaactc cttcggttga
     3481 gcctctaaaa gccctccatt ctccagataa ttaatatatg ccttaccact ctgactgcga
     3541 tcagcattac gggttatacc tgcaagttca ccgatcttct catcccaatg ttgctgggtg
     3601 attgcgacag ctctttctac tgcatgctga cgagaaaaat cagcaagcaa agacagagaa
     3661 tcttctaaaa acgcagcatg tcttgcgtca tagccattct caaaataatt tttgccatgc
     3721 tccttttccc attcattgat ccgggcagcg aatttatctg caacgtgctc aaacatagcc
     3781 tgttttgatg cttccagaat gccagatatc gtaccggtta atgcactcac cagcatgctt
     3841 atcggggccc ctatgaggga tgcactggag gcggcactaa ctcccgcaga tacagaactc
     3901 aggacagtat ttattgttgt cagggctgca tctatagctc cggtttcttt atgaaaggct
     3961 gcgagtaaag catccccttc ataattcaat tttttaaatc gttcagaata tgattcaagc
     4021 tgcttagctc gctcaaattt atctgcaata gccaggaaag aaagaggact gatagccagc
     4081 ataacagccg atgtgatcag acccgcactt gcagctgttg tcgataatcc ctgtgccatt
     4141 ctctgagcca gaatatattg cgaaacagct ttaccaacat ttccaagaac ctgagttgtc
     4201 agttcgatac ccgctgcagc ttttgttcct gtatgtgcgt cactgtttcc cagaataaag
     4261 cttgctgata cagcagaaag aattccggag acaacatcca gcccatcccc cagggggccc
     4321 aggttcggta aattttgtaa ttttccacca acagaactta agcgaggttt actggataaa
     4381 aatgagccaa gctggttaag ctgctcagaa aatgaatcaa cggtactgtt tatacttgat
     4441 actgtatcta caagctgatt aataagttca atactacttt tggcaatatc attctgactt
     4501 atatcctctc cttcccgttg ttttctcagc aattcatcaa gagccatgcc agataaagca
     4561 atccccgtaa aattctgtag tgctgagaga actgttccgg cttttcccag attattacct
     4621 acattttcag cggttcctcc tattttactc ccaactttct gatacttctg cagaagtttg
     4681 tcaagttgtg gtgcgaatat tgcaacacca cgttcagtta atcctacaac tttttctgct
     4741 gtaccaaaaa actggtttgc aatcgctgta tcatctcgtt cagtacggtg tatttctatt
     4801 ccaagatcat cagcagcttt gaccaactca ttaataccca cgccctgagc ttcataatta
     4861 tcaggtatta ataatatgag tttttttcca gcggaacgta cgcttgatga tgctgtattg
     4921 aatgctgatt ttgtagtcga tgtcgcattg ttgaaaatgt tctttatttt atttactgtc
     4981 atattagttt tctttctcaa tattaaaatt aaaatcttgt ctgtttttta cttcagacat
     5041 aagttcatga tggtattgcc tgaatatttt tgatgccagt tttttatcta tcccccctcc
     5101 atgaaattca gctattttcc ctgttttact gtactcatct aaccttattg ctctgaataa
     5161 ctcataagga tactttttcc tcatgtgttt atataactca atgttgtgtc caaatggagc
     5221 tatccagtca ataaaccact ttctttctcc cgacaaccag tcttttgcat acaaagatgt
     5281 tacatcattt atatatttta cctcacactc cagatctaag ctggcccagc tacaaaaagc
     5341 tacaggaagc tcatccttta ttaagagagc aaattgattt gtctgaatag caggtatgac
     5401 attaatagca aaaacagaca atggccattt tttatgcaga ggagaacagg cccatagcca
     5461 tgcaaccttc cccatgacgt caaaagcatt agatttcata atgtttaaat aaa
//
LOCUS       DAZFRN010000250         5443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFRN010000250 DAZFRN010000000
VERSION     DAZFRN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.252"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            14..4106
                     /locus_tag="ACHKUB_004863"
                     /pseudo
     CDS             14..4106
                     /locus_tag="ACHKUB_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
     gene            4598..4963
                     /locus_tag="ACHKUB_004864"
                     /pseudo
     CDS             4598..4963
                     /locus_tag="ACHKUB_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4973..5164
                     /locus_tag="ACHKUB_004865"
     CDS             4973..5164
                     /locus_tag="ACHKUB_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657760.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKEYVGEFALRGVCVPGGE"
     gene            5198..>5443
                     /locus_tag="ACHKUB_004866"
     CDS             5198..>5443
                     /locus_tag="ACHKUB_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO6657761.1"
                     /translation="MAYHSPDVTLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMHEQMSRIIRK"
BASE COUNT         1567 a         1177 c         1338 g         1361 t
ORIGIN      
        1 taacaggaat tatatgaata aaatatacgc gcttaaatat agctccctta ctggtgggct
       61 tatagctgtg tcagaattaa gtaagaaggt cacaggaaaa accgatagaa gattaatgac
      121 ggtttccctg gtattatcag tgactctttc tgctttaccg ggtaaagcat caacggtcag
      181 tgcagaaata ccatatcaga cttttcgtga ctttgctgaa aataaaggtg tgtttacccc
      241 cggagtgaca ggaattgaaa taaaagataa taatggaaat gcagttggta cgcttgatgt
      301 gccaatgatt gacttcagca gcgtgtcacg gcggggctca ctgaccctgt tgtcgcaggg
      361 atatggtgta tcagcaaaac acggagacct tggtgatgta aacaatgcca gctttggcta
      421 tgataaaaat aattatactg tagtaaagaa caacaaacat tcgggccttg atttttctct
      481 gcatcggttc agcaaactca tcgcagaggc tacacctgct gatataaata tatctggaca
      541 acttagtgat tcgtcacagt atactgcttt ttacagagca ggcgctggca cacagtatat
      601 taaagaacgc tcaggtaaac agactcatat cccgggaaca tttttaactg gtggaacagt
      661 aggcactcca tggtattcag gaaataattt aatatcaagc tctccaggtg acacctataa
      721 taaatcacag ggaccattag ccagttatgg gcagatgggt gacagcgggt cgccattatt
      781 tgcttatgat tctctcagtg aaaaaatggt ctctggctgg agtcaccctg cataactatg
      841 gggtgaacgg ttacagaaat aactggttgt tattacctga ggattatatc agaaatatta
      901 ttgtagccga tttcgatccc attatttcat ttaataaaaa cagtaaagaa catatgtcat
      961 ggacatatga tgcagccaaa ggcgtgggcc gtattcagca ggatgaccaa cagtttgtga
     1021 tgcatggtaa cctgaatggt aatctgaatg caggtaaaaa tctttatttt acaggcgaaa
     1081 atggcattat tgatttaaaa gataatgtta atcagggggc cggatatctt cagtttgctg
     1141 atgattatac agtaacaacg agcaatgaca gttcatggtc cggaggaggc attattgtca
     1201 attatggtac aactgtaaaa tggggtataa acggggtttc cggagatgac cttcataaag
     1261 ttggggacgg aacgcttatt attaatggaa ccgggaaaaa tgaaggggga ctaaagattg
     1321 gggctggtac tgtaatcctt gaacaaaaag cgaaaaacaa cgattcaacg gctttttctt
     1381 ccattaacat cagtggggga aactcgcgcg ttaaactttc aggagataat cagattatac
     1441 ctgataatgt ttcctgggga ttccgggggg gatatttaga tataaacgga aaagacactg
     1501 agttctcccg gttacaggct gttgattatg gggctgcaat tattaacagc agcacagaca
     1561 agtcattatt gacgttaaat ctgtcaccat tgaaaaaaga tgaaatagct gtaagcgtga
     1621 aggcgttaga tatgaacgcc atctttcagg ggggacacgg gacagccgga gatttgtata
     1681 aaacaacttt ttacggtccg acacagtatt atctgctgaa aaagcccaaa tttggcagtg
     1741 tgctgatggg gtcactgaaa aacaccagcg aatggcaatt tgcaggaaca gacctgaatc
     1801 aggccgttga tatggcaaag aataataaac tgacatcaag tgcacaggct tcatatcttt
     1861 atcacggtaa gctcctgggg aatatggata ttgtcattcc ggagcttaca ggcaatgata
     1921 ttttaacgct tgatggctct gtcagtatat ccggggatat gtcaaaacag gacggtgctc
     1981 ttatcttcca gggacacccg gttattcatg cagggcaaac tgtttctgca tcgcagagtg
     2041 actgggagaa cagggagttc tcactcaaca atctgaatct taataatgcg gacttcagtc
     2101 tgtcccgtaa tgcatttatg aacgggaata tcagggccgt taaccagagc actgttatta
     2161 tcggcggaga tacagtcttt actgataaaa atgacggaac aggtaatgat gtcatcagtg
     2221 ttgaagggaa atctgctgcc gcaggaacat cctcctatac agggcatatc actctggagc
     2281 aaaaatcagc actggatatc cgcgataatt ttcgtggcgg ggttacgtct gaagacagtc
     2341 atatcaatgt ttcttcatct tcagtcctgt tctcagatgc atcgtcattt ataaacagct
     2401 ccctgaatat tcataaagga ggtgcgctga ccgctcaggg agggctgttt acaagtggaa
     2461 gcattgatat tggtgacgct tcccttctgc ttaccggtac accagtgaat tcagatgatg
     2521 ctgctttttt accgaccatc aatatggctg atggcggatt taaactgatg tctgattcat
     2581 cagtactgaa agccagagac caggcatctg ttgttggtga tattatttct gataaacagg
     2641 ccacaatcag cttcggaact gaatcaggta aagagggcat attgtctgag aaggcatccc
     2701 ggggactcgc ggtaggatta ctgagtggtt ttaatacggc ataccgcggt gcaattcatg
     2761 ccccgtcagc atctgccact atgaacaaca cctggtggca actgacagga gactcctcac
     2821 ttcgctcgtt aaaaaatacc ggaagcatgc catattttac aggaagtgca gcgaataaag
     2881 cattccatac actgacggtt gatgagctga cgacgaatgg cactgcgtat gccatgcgta
     2941 cggacctgaa aaatgcggat aagctggtag taaaccaaaa gctgtcaggt aaggacaata
     3001 ttctgctggt tgattttctg aacaaaccca ccggagaaaa actggatatt gaactggtga
     3061 gtgcaccggg gaacagcagt aaggatgttt ttaaaggaag tgaacaggca ataggtttca
     3121 gcaatgtcac acctgttatt acagctaaag acgccggaga taaaacaaca tggaacctga
     3181 ccgggtacag gatggcagaa aatcccgccg caacccaaag tgcctcaggc cttgcatctg
     3241 tggggtacaa atcatttttg agtgaggtca acaacctgaa taaacgtatg ggtgacctgc
     3301 gtgacatcaa tggtgaagct ggcgcatggg cacgtatcat gagcggaacc ggctctgccg
     3361 gtggtggttt cagtgacaac cacacacatg ttcaggtcgg tgtcgacaaa aaacatgagc
     3421 tggacggact ggatttgttt accggcttca ctgtcacaca cactgacagc agtgcctctg
     3481 ctgatgcttt caaaggtaaa acaaaatctg tgggggccgg actctatgct tccgccatgt
     3541 ttgattccgg tgcctatatc gacctgattg gtaagtatgt tcatcatgat aatgagtaca
     3601 ccgcaacctt tgccggactc ggaatccgtg attacagtac gcattcatgg tatgccggtg
     3661 ctgaagcagg ctaccgctgt catgtcactg aggatacctg gattgagcca caggcagaac
     3721 tggtttacgg tgctgtatcc ggtaaacagt ttgcatggaa ggaccagggg atgcatctgt
     3781 ccatgaagga cagggactac aatccgctga ttggtcgtac cggtgtggat gtgggtaaat
     3841 ccttctcagg taaggactgg aaagtgacag cccgtgccgg tctgggctac cagttcgacc
     3901 tgctggctaa cggcgagaca gtattgcggg acgcatcagg tgaaaaacgt atcaaaggtg
     3961 aaaaggacag ccgtatgctg atgtccgttg gcctgaatgc agaaatcagg gataacgtcc
     4021 gctttggact ggagtttgag aaatccgcct ttggtaagta caacgttgat aatgctgtca
     4081 acgctaactt ccgttactcg ttctgatact ccgtattcaa aagggcctga gggcccttta
     4141 tgctcaggac ctgccgcccg ggtatacatt tccggagtat cagtcgattt gtttacttaa
     4201 ccgccatgac caaagccggc agtaatgtcg gctttttacg tccatatatt ccactcatcc
     4261 ggaactgtga cacagaacct gaatacagga ttgatcaccg gtgcccataa atggcacatt
     4321 ttcagtaaga ttctgaatgt tacattcccc cttcatgtgt gacacagctg tcacagagaa
     4381 cgaaacggaa agcacatgaa aaacataaaa actcacaccg gactccttat ccacaaagaa
     4441 caaacacggc gcgttcgtct tcatgaaaca ccggccgcct ggtgccacac acgccgggaa
     4501 tgctacagca aaacgaccgg tcgtcgctgc ggcagccctg acagtctcag caggctgatt
     4561 ctgagcagta ttcagcccat agaatgagat aacccgtatg aatgatgaca gacagacgtc
     4621 tacggtaccc gatgtatgga acatttgctt tctggctaac ggcatgcccg acacatggtt
     4681 ttacaccgat gacgcctccg tcacaaaaga gtgggggcgg gaatattgtg acaatgtgga
     4741 agtgttccct ctgtgctata tgcatgacgg tcccgttttc cgggggcgct atattgatga
     4801 gctcagcgcc gcacggaaca gaatgctcca tatgcaccac atactgccgg aatgaacccg
     4861 gtacagagaa aattttcatg acgcagaatg tatccgtgaa attcacgaag cgcccctgca
     4921 ggctcccggc attctgcaaa aatacaaacg cacaaaatca taaggctgtt ctatgtggat
     4981 actgattttc tggctctcat ccccctttaa tgtctcctcc ggcgatgccc ggcaacccac
     5041 actggccgtt cacagtcagg agttccgtac tgaaaaagcc tgtcgggatg ccttcagcgc
     5101 gctgaaaaaa gaatacgtgg gagaattcgc tctccggggc gtctgcgtcc ccggcggaga
     5161 ataaaccgga gatgatgatt ctccacagga gtattcaatg gcataccatt ccccggatgt
     5221 cacactgcat tatccgtatc tgcgccggat gtcctgggcg cagataacgg cagcagctga
     5281 agatgcggaa atacatcatg attatgcccg ggcattaata ttgtggcagc atgcatatca
     5341 tgctgccacg ctgactctca acaaaaacct ggcagttgct aaaatcgact tctgtattaa
     5401 acgtatccgg atgcatgaac aaatgagcag gataatccgg aag
//
LOCUS       DAZFRN010000251         5370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFRN010000251 DAZFRN010000000
VERSION     DAZFRN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.253"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..92
                     /locus_tag="ACHKUB_004867"
     CDS             <1..92
                     /locus_tag="ACHKUB_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6657762.1"
                     /translation="VMVNTGGNKEQVPAKRQNRGSRNSTDDGG"
     gene            116..637
                     /locus_tag="ACHKUB_004868"
     CDS             116..637
                     /locus_tag="ACHKUB_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6657763.1"
                     /translation="MAVRGINKVILVGRLGKDPDVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            695..928
                     /locus_tag="ACHKUB_004869"
     CDS             695..928
                     /locus_tag="ACHKUB_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO6657764.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNRYIRDFGILK"
     gene            989..2947
                     /locus_tag="ACHKUB_004870"
     CDS             989..2947
                     /locus_tag="ACHKUB_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6657765.1"
                     /translation="MSVTKSEPKSTRKASRKLAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVCVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMESVGFCREDAGTYRCLPEPESVLTEAEE
                     ERLNELMARYDALENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDPAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPVGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMGSPRPQTDATERADNLADAA"
     gene            3002..3436
                     /gene="psiB"
                     /locus_tag="ACHKUB_004871"
     CDS             3002..3436
                     /gene="psiB"
                     /locus_tag="ACHKUB_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO6657766.1"
                     /translation="MKTELTLDVLQTMSAQEYEDIRAAGSGERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3433..4152
                     /locus_tag="ACHKUB_004872"
     CDS             3433..4152
                     /locus_tag="ACHKUB_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6657767.1"
                     /translation="MSVRSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRWISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERRRHKSALVMQKYSRQQAREAEQKTRAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4149..4463
                     /locus_tag="ACHKUB_004873"
     CDS             4149..4463
                     /locus_tag="ACHKUB_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFO6657768.1"
                     /translation="MKWRYSLRWKLPYPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            4613..4765
                     /locus_tag="ACHKUB_004874"
                     /pseudo
     CDS             4613..4765
                     /locus_tag="ACHKUB_004874"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            4710..4832
                     /locus_tag="ACHKUB_004875"
     CDS             4710..4832
                     /locus_tag="ACHKUB_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6657769.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
BASE COUNT         1166 a         1427 c         1701 g         1076 t
ORIGIN      
        1 aggtgatggt gaataccgga gggaacaaag agcaggtccc cgcaaaacgt caaaaccgcg
       61 gctcccgaaa ctcaacagat gatgggggct gaaaacatca acagaaggag acatcatggc
      121 agttcgtggc attaacaagg tcattctcgt gggacgtctg ggaaaagatc cggatgtccg
      181 ttacatcccc aacgggggcg cagtggcaaa tctgcaggtg gccacgtcag aaagctggcg
      241 tgacaaacag acgggggaga tgcgggagca gacagaatgg caccgcgtgg tgctgttcgg
      301 caagctcgcg gaagtggcag gtgaatatct gcgtaagggc gcgcaggtct acatcgaggg
      361 gcaactccgt acccgtagct gggaagataa cggtattacc cgttacgtca ctgaaatcct
      421 tgtgaaaacc acgggcacca tgcagatgct ggggagtgca ccacagcaga acgctcaggc
      481 gcaaccggag cctcagcaga atgggcagcc acagagtgct gacgcgacga aaaaaggtgg
      541 cgcgaaaacg aaaggccgtg gacgtaaggc cgcgcagccg gagcctcagc agccgtctga
      601 acccgcgtat gacttcgatg acgatattcc gttctgaacg ggctgactgt gataaccgcc
      661 ccgtcctgcg cggggcatca ccggagaaat gaggatgagc gaatatttca gaatacttca
      721 gggactgccg gacggctcct ttacccgcga acaggcggaa gccgttgccg cacagtaccg
      781 gaacgtcttt atcgaggatg atcagggaac gcattttcgc ctggttgtcc gtcaggatgg
      841 cacgttgatc tggcgctcct ggaattttga ggactgtgcc gggtactgga tgaaccggta
      901 catcagggat ttcgggatcc ttaagtaaga gaggtgccgg acggggatca cgtccggcag
      961 acattaacta cacaataagg ggatagtcat gtcagtaaca aagtctgaac caaaatctac
     1021 ccgtaaagca agccgtaaac ttgcaaaaac gcaggaaact gtcctgtctg ccctgctggc
     1081 gcagacggag gaagtgagcg tgccgctggc ctcactgatt aaatcaccgc tgaatgtgcg
     1141 cacggtgccg tattctgcgg agtccgtcag tgaactggct gattccatta agggagtcgg
     1201 cctgctgcag aatctggtcg tgcataccct gcctggtgac cgttacggtg tcgccgcagg
     1261 tggtcgccga ctggctgcac tcaacatgct ggcagagcgt ggcatcattc cggctgactg
     1321 gcctgtctgt gtgaaggtca ttccgcagga actggcgacg gcggcatcaa tgactgaaaa
     1381 cggtcatcgt cgggatatgc accctgctga acagattgcc ggattccgcg caatggcgca
     1441 ggaaggcaaa acacctgcac aaatcggtga tttgctgggc tattcacccc gccacgttca
     1501 gcgaatgctg aaactggcag accttgcacc agtcatactg gatgcgctgg cagaagaccg
     1561 catcaccacc gaacactgtc aggcgctggc actggagaac gacaccgcgc gtcaggtgca
     1621 ggtgtttgaa gccgcctgtc agtcgggatg gggcggtaaa ccggaagtac agaccattcg
     1681 tcgtctggtg accgaaagtg aagtggcggt ggcaggcaac agtaaattcc gcttcgtggg
     1741 ggctgatgct ttctcacctg acgaactgcg caccgatttg ttcagcgacg acgagggtgg
     1801 ctatgtggac tgtgttgcgc tcgatgctgc cctgctggaa aaactccagg ctgtcgctga
     1861 acaccttcgg gaagctgaag gctgggaatg gtgcgccgga cgtatggagt ctgtcggttt
     1921 ctgccgtgag gatgccggaa cataccgttg tctgccggag ccggaatcgg tgctgaccga
     1981 ggcggaagaa gaacgcctga acgaactgat ggcacgttac gacgcactgg aaaaccagtg
     2041 tgaggaatct gacctgctgg cagcagaaat gaagctgatt gactgcatgg cgaaggtcag
     2101 ggcgtggacg ccggagatgc gtgccggaag tggcgtggtg gtgtcctggc gttatggcaa
     2161 cgtatgtgtc cagcgtggtg tgcagttgcg cagcgaggat gacccggctg ccgacgctga
     2221 ccgcacggaa caggtgcagg agaaagcgtc agtggaggaa atcagtctgc cactgctgac
     2281 gaaaatgtct tcagagcgca cgctggcagt ccaggcggcg ctgatgcaac agccggacaa
     2341 atccctggca ctgctggcat ggacgctctg cctgaatgtg tttggcagcg gggcgtacag
     2401 taaaccagca caaatcagcc tggaatgtga acattattcg ctgaccagcg atgcgccatc
     2461 ggggaaggaa ggtgccgcat tcatggcgct gatggcagaa aaagcccgtc ttgctgccct
     2521 gttacctgtg ggatggtcac gggacatgac gacattcctg tcactcagtc aggaggtgct
     2581 gttatccctg ctcagtttct gcaccgcatg cagccttaac ggtgtccaga cccgtgagtg
     2641 tggtcacacg tcacgcagtc cgcttgactc gctggagagc gccatcggct ttcacatgcg
     2701 tgactggtgg cagccgacga aagcaaactt cttcggacac ctgaaaaagc cgcagattat
     2761 cgcagccctg aatgatgccg gactgtccgg tgccgcacgg gacgcggaga agatgaagaa
     2821 aggcgatgcc gctgaacatg cagagttcca catgaaagac aaccgctggg tgcctggctg
     2881 gatgggttca ccacgtccac agacggatgc cactgaacgc gccgataacc tggctgatgc
     2941 cgcctgatga acaaccacac cgccccgccg gagacggggc ggcagcaagg gagataccgt
     3001 gatgaaaact gaactgaccc tggatgtatt acagaccatg agcgcacagg aatatgaaga
     3061 tatccgggct gccggaagtg gtgaacgtcg tgagctgaca cacgccgtga tgcgggagct
     3121 ggatgcgccg gataactgga cgatgaacgg cgagtacggc agcgagtttg gcggcttttt
     3181 ccccgtccag gtccgtttca cgcctgccca cgaacgtttt cacctggcgt tatgttcacc
     3241 gggtgacgtc tctcaggtct gggtgctggt tctggtgaat gccggcggtg agccgtttgc
     3301 ggtggttcag gtgcagcggc ggtttgtacc ggaagccgtc agccattcac tggcactggc
     3361 ggcgtcactg gatgcgcagg ggtacagcgt cagtgacatc atccatatcc tgatggcgga
     3421 aggaggtcag gcatgagcgt acgttcacag gcactggttc ccctcagcgc agagcaacag
     3481 gccgcatggc gggcggtggc ggagacggaa aaacgccgcc atcagggaaa cacgctggcg
     3541 gaatatccgt atgcgggggc gtttttccgc tgtctgaacg gaagccgctg gatttcgctg
     3601 tctgacctgc gatttttcat gccgtccctg accgcagaag aactgcacgg caaccgtctt
     3661 cagtggctgt atgccatcga tgtgctgatt gaaacacagg gcgaggtctg cctcctcccc
     3721 cttcccggcg atgcagcaga acgcttgttc ccgtcggtgc ggttccgtgt cagggaacgc
     3781 cgtcgccata aatctgcgct ggtcatgcag aagtacagcc gccagcaggc gagggaggca
     3841 gagcagaaaa cccgtgcgta ccaggcgctg gttgctcagg cagaaatcga actggcgttc
     3901 cattcaccgg aaacagtcgg aagctggcat gcacgctgga gtgaccgggt ggcagagcat
     3961 gacctggaaa ccctgttctg gcagtggggc gagcgttttc cgtcactggc cggaatggaa
     4021 cgctggcagt ggcaggatat gccgttctgg caggtcatcg cagaggcagg tatggcggca
     4081 agggaggccg gtcatgccgt ccgtgagatg gagcgatgga tggtgccgaa caaactccgg
     4141 gaggcggcgt gaagtggcgt tactcactgc gctggaaact gccttatccc tgtcctggcg
     4201 agcatgaact ggtttcagaa gtggtggagg cggggcagcc tgcaccggca tccgttatgt
     4261 cacgctgggt tgccggagca gggtatgcgg tctgtctgga ttttatttct gaccgcccgg
     4321 tgcgccgctg gagcgaagaa cgtaaggctg cggtacggcg gcggaatctg gagaaacgta
     4381 tcaacaggca tgcaccactg tttgcggatg aactgattgc ccgtgaactg gctgaacgac
     4441 cggattattt tcagggaaag tgagtgtggt cagtacgcag gtatatgggc tatgatgtgc
     4501 ccggcgcttg aggctttctg cctcatgacg tgaaggtggt ttgttatcct gtcgtgtggc
     4561 agaaggacaa aagccccgta gttaattttt cattaaccca cgaggcctct gcatgtctag
     4621 tccacgtcag tatggcctct taccgtgcaa attgcaagga ggagtaaacc atgaaactac
     4681 cgggaaacgc ccttatctgg tgcgtattaa tcgtgtgctg cacgttgttg atattcacac
     4741 tcctgacccg gaatcgcctg tgcgaagtcc ggctgaagga cggatacagg gaagttacgg
     4801 caacaatggc ttacgaatcc ggcggtaagt agcaacctgg aggcgggcgc aggcccgcct
     4861 tttcagggct gatgctggtc tgactgcact gaagcgcctt tataaagggg ctgctggttc
     4921 gccggtggcc cctttctcct tgctggcatt gtagcagcat gaacaaccag actgtcagga
     4981 aggccacttc cctccgggaa cgggaatgaa cgtgcgcctg cggcgctcgt tcttttcccc
     5041 cgccttctct gtttatggcc agttgtcaga aatgacatta cctttcccgt gattattctc
     5101 tttttcttcc tgctctgatt ctgactactg cagtgttgtc ctgtctgccg ggcgcacctt
     5161 cggctcccgg ctgcctggcg gcatccggtc gtcaggcccg actccgacag gcagctgctg
     5221 gcgcgcctgc cagtctccgc cgaacccgct ggcgcggttt cgggctgccg ctacggtccg
     5281 atgcgtgaaa accccgggct tgcagcgacc gttccggtcg cgccgcctct gtcgcgccgt
     5341 ggcctgcgct cccggcatcc cggcaaaaag
//
LOCUS       DAZFRN010000252         5342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFRN010000252 DAZFRN010000000
VERSION     DAZFRN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.254"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..227
                     /locus_tag="ACHKUB_004876"
     CDS             <1..227
                     /locus_tag="ACHKUB_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657770.1"
                     /translation="RVRKNYILQCGPGRFRNRKKLNALLNILESQFRLSVVPEGKTMY
                     VLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(549..1151)
                     /locus_tag="ACHKUB_004877"
     CDS             complement(549..1151)
                     /locus_tag="ACHKUB_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657771.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFIRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLSYRTTVDDKRT"
     gene            complement(1246..1452)
                     /locus_tag="ACHKUB_004878"
     CDS             complement(1246..1452)
                     /locus_tag="ACHKUB_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6657772.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            2195..2332
                     /locus_tag="ACHKUB_004879"
     CDS             2195..2332
                     /locus_tag="ACHKUB_004879"
                     /inference="COORDINATES: protein motif:HMM:NF017162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulating family protein"
                     /protein_id="HFO6657773.1"
                     /translation="MIFHTRYRLTPVDLEARNSAVAHRLTELTANFTIVFLLPSGHLN
                     F"
     gene            complement(2671..2871)
                     /locus_tag="ACHKUB_004880"
     CDS             complement(2671..2871)
                     /locus_tag="ACHKUB_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657774.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            3071..3640
                     /locus_tag="ACHKUB_004881"
     CDS             3071..3640
                     /locus_tag="ACHKUB_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6657775.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEYTKAHCTGERNFGCSRS"
     gene            3900..4301
                     /locus_tag="ACHKUB_004882"
     CDS             3900..4301
                     /locus_tag="ACHKUB_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6657776.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            4289..4723
                     /locus_tag="ACHKUB_004883"
     CDS             4289..4723
                     /locus_tag="ACHKUB_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO6657777.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            5078..>5342
                     /locus_tag="ACHKUB_004884"
     CDS             5078..>5342
                     /locus_tag="ACHKUB_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6657778.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAE"
BASE COUNT         1327 a         1319 c         1438 g         1258 t
ORIGIN      
        1 cccgtgtcag gaaaaattat attctccagt gtggcccggg ccggttcaga aacaggaaaa
       61 aactgaacgc attgctgaac attcttgaat ctcagttcag gctttctgtc gtgccggaag
      121 gaaaaaccat gtatgttctg cttccccaga tagccagttt aaagttgtct gatgtctctg
      181 gtatttttac ctcgggttat cactacaata agctgagagc gaagtaaccg gtgagcagtt
      241 tttctccatt atgcttcaga cttagatgat tgattattat ggggatttca tcaccgatat
      301 tcagatgtat gaatatcggt gatgaactaa agctcaggag ctgcgtggtg gtgatgaatc
      361 aacaggcata taactgatac ccgccggtca gaagttccag cctgcgactt tcctgtttgc
      421 gtgcccgcag tccggcgttc aggtgcagcg tgtgacagaa cgcacggaga gcgaaacggc
      481 aggcagacag gccgttcagt cagccctggc accctgcccg gataccagct tccctgaaac
      541 aactggcatc aggtgcgctt atcgtcaacc gttgtgcggt atgacaggag cacgtactga
      601 tccatcagat ccgtcagtgc tgcgctctgc ttctgacggt tgatgatcca ttcctccagc
      661 tctttttctg atttccgttt cagattctcc gtccagtcgt caaggcagtt tccggtcatt
      721 aaatcaagcc agcaaagcca gactagtttg aggcgcaact ccgtgccacc aaactgacgg
      781 aagaggctga tcagtttatt tgccagagaa cgctgcctca gacgttcaca ttcagacagg
      841 agaatgcctt ccagcatcgg atgatgaacc agcagatgtt cactggtgca taaattcact
      901 atcagatcat catccagggg cggtgtggcc cgtttgtcag ggccataaat cagccggtgc
      961 aggctctggg gtacgatggc aatctccggt ttttcaccat tgaatgacac ccaaccgggc
     1021 gtcagattat ggaagtagta gatattcgga tgacacacgc cccaggatac ggcgtgccgg
     1081 atgaagccgg tcagccattt ctggtagctt tcgtggagcc attcgtagcg ggtctgatac
     1141 tgttgtttca ttttcatatt cctcacgggg atataacagg cagcagaatg ccgccggcag
     1201 gccggagatt acgtctgctg cgggtgaacg gataacggtc agaaattacg gacgggactg
     1261 tgcaatacgc gcctgcagcc aggcttccac ttcacttttc agccagcggg agctgcgctc
     1321 cattttgatg ggggcaggaa agcccccatc tttgatgagt ttgtaaaacc acttatcggt
     1381 taagccggta agttgagtga taaacgccat gtcgaccatc tggtcatcca tcagcgaaac
     1441 aggggtagcc atgagaatgt tcctccggat gttgttaaac cagccggtgt ggtcatgaaa
     1501 acagcgcgcc accttcactg gcataacaac cggggagaaa cgtaaaaaat aatcgggaat
     1561 gtggctgtcc ggagataaac ggggggattg cgatggcaga gccgtagcgg aatcccgggt
     1621 ggttgttgcg aaaagatgct tccgcacgca cgcgcgataa aggagggagg actttcgtga
     1681 gacaagggga aaactagcgt tctgataatt gcagagttgt tttcggaggt ggttacagtg
     1741 agtcagcccg ttcctggcga ggaggagact gatatcaccg atgggataag gctgtctgat
     1801 tggtattata aatataagaa gtcatcagag agtaacgtaa aggatttgcc agagtaagct
     1861 aagagagtaa atctctccgg taacagagcg ctgtgaagca ttctgtatca gagtgcaaat
     1921 acatgaacgc tgatgcgtaa aatcagcagt gacacgccag ctccccctgt gcccattcct
     1981 gtataagaca tgaactgagc gtattaatgc tcctgctctg agactgagcc ggtgagtgag
     2041 gggctgagaa atccctcttg acacagctct aaaccagaaa tacttccccg gttgttgcgg
     2101 tgcgtccgga tccccgataa cggaacacta aggctgtatt gcgggacacc gggtaagtgg
     2161 cagcctgtaa tgtgttctgt caacgccaga aaaaatgatt tttcacacgc gttatagact
     2221 cactcctgtg gacctggaag cccgtaactc tgcggttgcc caccgactga cggaactgac
     2281 cgcaaacttt acgatcgtgt tcctgcttcc ttctggtcac ctgaactttt agccagcgct
     2341 acgataagtc cggtgttatc tgcaccggag gaccgggaac attaagcggt atagcgacaa
     2401 caaagaattc gaaatcaaaa gggaatggtg ccggaggcgt gggcggtaat aggctgattt
     2461 tcaggtatta ccggcttaca acgtgtggtt gatattgagg ggctgtgagt ttcgcgtatt
     2521 tgtcagaaac ccagatgagt tttcctttat gggacgaaat aaggcagcgg caatacctgg
     2581 aaaaatttaa tatggtatga aggagaacct gtcggttacg cttaatatac acggtacact
     2641 gtcagctcag gggatacatg aaccgttatc tcaccagcta cacagtatat acacactgac
     2701 gctgacagta gtcagcagga ccacaataat cagcgccagc agtatggtaa cgatacggtc
     2761 tgtattccgg tagtagccga aggggtcaac agaaccacaa tacgggcaac acactgtatt
     2821 atgagtaatc gcgtttccac agtccctgca cttctttttg cgtatcacca tcactccctc
     2881 ctcacagcct tacccgtaac cgatttttta cattaaaaaa acaacgctga aaattcaacc
     2941 gtcagtccgg agacgaccgt tcgggttatc acagagtgcc tgagacagtg tcctgccgga
     3001 ggtcacttca gggactctgc gcatttttta cgacgtggct attccgtatg aaccatacgg
     3061 agatttaacc atgacctaca aatacaaccc cttctggcag caacgtattc gtgagacggt
     3121 gcggcacgca ctgaatgttc atccccgcct gacggcattg cgggttgacc tgcgtctccc
     3181 ggatgtaccg gcagcaacgg acgcagctgt gatatcccgc ttcatcaatg ccctgaaagc
     3241 ccgaatcgac gcttaccaga aacgtaagca tcgggaaggt aaacgcgtgc atcccacaac
     3301 cctgcattac gtctgggccc gggagtttgg ggagtgcaaa ggtaaaaaac actatcacct
     3361 gatgctgctg gtcaaccggg atacctggtg tcgtgccggt gattaccgcg ctccggagtc
     3421 tctggccggg atgattaaac aggcgtggtg cagtgccctg ggagtggatg tcgggtgcca
     3481 tgccacgctg gtgcattttc cggcctggcc ggcggtgtgg ctggcgcgta atgatgacac
     3541 cggctttcag caagtgctgg aacgtgctga ctatctggcg aaggagtata ccaaagctca
     3601 ctgcaccggt gagcgcaatt ttggctgtag ccggagttga gcacgactgg cgcgccaata
     3661 cccggcaaac caaaagtatg ggttgttgtc tggcggcaac gatgtatcca ctcgcccact
     3721 gactgttttt tacgtcagtg ttccattgtg tgacaaccac agtgctgtaa cagcaaccag
     3781 cctgctttac ccaaactcca gtccagattt taactgaacc gccattcaca ccctgaccac
     3841 gctgcccgta ccgggacggc tgttgcctgc gtgtcgtctg gcggctgagg aatatgacta
     3901 tgtacgcaaa atcctttatc gctcttgatg gcaacggacg tctgacgggc gcccgtactg
     3961 cacaggccgc accttatgct aactacacct gccacttgtg tggcagtgca ctcagatacc
     4021 atccgcaata cgacactgaa cttccctggt ttgaacacac tgacgacagg ctgacagagc
     4081 acggtcaaca gtgcccttat gtcaggccgg agcgcagaga aatacagttg attaaacgtc
     4141 tgcagcaatt cgtaccggat gccttacccg tggtgcgtaa agccagctgg cactgcagac
     4201 aatgtcacca cgattattat ggggagcagt actgcacaca ctgccagacc ggaggattca
     4261 gtattccccg gacaactcag gaggaaatat gcgaattctg aactgttata tggcgaatga
     4321 cagcaaaggc cattttgtta cggcgaaaga agctgcgaag cacaaccgac aggacgtttt
     4381 gtgctgtgtg tcctgtggat gcccgttaac acttcagcgg ggcaatgacg gacaaccacc
     4441 gtggtttgaa catgaccaga tgactgtcgc tgaaaaaatc ctgctgcgat gtacctggct
     4501 tgacccggcg gagaaagagg cccgtcgttt gcatctgcag ggcatgacgg ttccggatta
     4561 tacggtgaag gtgagaaagt ggttttgtgt gatgtgtgac gaagattatg agggggaaaa
     4621 gtgctgccca cgctgcggta ccggggtata cagcagggcg tgggggcggc aggaggtgcc
     4681 gtcggaagat gccagggctg ataatccgtt acagaggctg taatggttgc ctccggagca
     4741 gtttgcggtg atgcctttcc cgggaatgga ttgccagtgc ggggactgtg gagcttaatt
     4801 ccggtgttgg tggcctttcc gttgatttta tgccaacagc cccctgtgac tgacaggctg
     4861 cctcgtcatt ccattcgtat ctccagtaac agggggttgt attgtatttt attgtgccgg
     4921 ttcaggtgtg cgtttcccgg cgcgtctgca ccggcttaac caaattcaac agggataaaa
     4981 tagaggtaag cgtacagcct gaaccgtctg gtcagaatct gacgaattag acaaagtggt
     5041 gtccaccaaa taagtagtgg gaaccaaagt gtcagatatg cagaaaaatg tgactcccgg
     5101 caggcgaaaa ggctgcccta attatcctcc cgaatttaaa cagctgctcg ttgctgcctc
     5161 ctgtgaaccc gggatatcca tctcaaaact tgctcttgaa aatggcatta acgccaatct
     5221 gttgttcaaa tggcgacaac aatggcgcga gggaaagctg ctattacctt cttcagagag
     5281 cccccagcta cttcctgtga ctctcgatgc agctgccgaa cagccagaat cgctcgcaga
     5341 gg
//
LOCUS       DAZFRN010000253         5284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFRN010000253 DAZFRN010000000
VERSION     DAZFRN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.255"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            261..2966
                     /gene="malT"
                     /locus_tag="ACHKUB_004885"
     CDS             261..2966
                     /gene="malT"
                     /locus_tag="ACHKUB_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO6657779.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3009..4025)
                     /gene="rtcA"
                     /locus_tag="ACHKUB_004886"
     CDS             complement(3009..4025)
                     /gene="rtcA"
                     /locus_tag="ACHKUB_004886"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO6657780.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4029..5255)
                     /gene="rtcB"
                     /locus_tag="ACHKUB_004887"
     CDS             complement(4029..5255)
                     /gene="rtcB"
                     /locus_tag="ACHKUB_004887"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO6657781.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT         1241 a         1487 c         1333 g         1223 t
ORIGIN      
        1 ggcactttca tcaggaatga agaagaaacc cttgcttaaa tgaatctgat gaacataagg
       61 gaaaccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aagatttgga
      121 attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt
      181 ttctggccga ccttataacc attaattacg aagcgcaaaa aaataatatt tcctcatttt
      241 ccacagtgaa gtgattaact atgctgattc cgtcaaaact aagtcgtccg gttcgactcg
      301 accataccgt ggttcgtgag cgcctgctgg ctaaactttc cggcgcgaac aacttccggc
      361 tggcgctgat cacgagtcct gcgggctacg gaaagaccac cctcatttcc cagtgggcgg
      421 caggcaaaaa cgatatcggc tggtactcgc tggatgaagg tgataaccag caagagcgtt
      481 tcgccagcta tctcattgcc gccgtgcagc aggcaaccaa cggtcactgt gcgatatgtg
      541 agacgatggc gcaaaaacgg caatatgcca gcctgacgtc actcttcgcc cagcttttca
      601 ttgagctggc ggaatggcat agcccacttt atctggtcat cgatgactat catctgatca
      661 ctaatccagt gatccacgag tcaatgcgct tctttattcg ccatcaacca gaaaatctca
      721 ccctggtggt gttgtcacgc aaccttccgc aactgggcat tgccaatctg cgtgttcgtg
      781 atcaactgct ggaaattggc agtcagcaac tggcatttac ccatcaggaa gcgaagcagt
      841 tttttgattg ccgtctgtca tcgccgattg aagccgcaga aagcagtcgg atttgcgatg
      901 acgtttccgg ttgggcgacg gcactacagc taatcgccct ctccgcccgg cagaataccc
      961 actcagccca taagtcggca cgccgcctgg cgggaatcaa tgccagccat ctttcggatt
     1021 atctggtcga tgaggttttg gataacgtcg atctcgcaac gcgccatttt ctgttgaaaa
     1081 gcgccatttt gcgctcaatg aacgatgcac tcatcacccg tgtgaccggc gaagaaaacg
     1141 ggcaaatgcg cctcgaagag attgagcgtc aggggctgtt tttacagcgg atggatgata
     1201 ccggcgagtg gttctgctat cacccgctgt ttggcaactt cctgcgccag cgctgccagt
     1261 gggaactggc ggcggagctg ccggaaatcc accgtgccgc cgcagaaagc tggatggccc
     1321 agggatttcc cagcgaagcg attcatcatg cgctggcggc aggcgatgcg ctgatgctgc
     1381 gcgatattct gcttaatcac gcctggagtc tgttcaacca tagcgaactg tcgctgctgg
     1441 aagagtcgct taaggccctg ccgtgggaca gcttgctgga aaatccgcag ttggtgttat
     1501 tgcaggcgtg gctgatgcaa agccaacatc gctacggcga agttaacacc ctgctagccc
     1561 gtgctgaaca tgaaatcaag gacatcagag aagacaccat gcacgcagaa tttaacgctc
     1621 tgcgcgccca ggtggcgatt aacgatggta atccggatga agcggaacgg ctggcaaaac
     1681 tggcactgga agagctgccg ccgggctggt tctatagccg cattgtggca acctcggtgc
     1741 tgggtgaagt gctgcactgc aaaggcgaat tgacccgctc actggcgcta atgcagcaaa
     1801 ccgaacagat ggcacgccag cacgatgtct ggcactacgc tttgtggagt ttaatccagc
     1861 aaagtgaaat tctgtttgcc caagggttcc tgcaaaccgc gtgggaaacg caggaaaaag
     1921 cattccagct gatcaacgag cagcatctgg aacagctgcc aatgcatgag tttctggtgc
     1981 gcattcgtgc gcagctgtta tgggcctggg cgcggctgga tgaagccgaa gcgtcggcgc
     2041 gtagcgggat tgaagtcttg tcgtcttatc agccacagca acagcttcag tgcctggcaa
     2101 tgttgattca atgctcgctg gcccgtggtg atttagataa cgcccgtagc cagctgaacc
     2161 gtctggaaaa cctgctgggg aatggcaaat atcacagcga ctggatctct aacgccaaca
     2221 aagtccgggt gatttactgg caaatgaccg gcgataaagc cgccgctgcc aactggttgc
     2281 gtcatacggc taaaccagag tttgcgaaca accacttcct gcaaggtcaa tggcgcaaca
     2341 ttgcccgtgc acaaatcttg ctgggcgagt ttgaaccggc agaaattgtt ctcgaagaac
     2401 tcaatgaaaa tgcccggagt ctgcggttga tgagcgatct caaccgtaac ctgttgctgc
     2461 ttaatcaact gtactggcag gccggacgta aaagtgacgc ccagcgcgtg ttgctggacg
     2521 cattaaaact ggcgaatcgc accggattta tcagtcattt tgtcatcgaa ggcgaagcga
     2581 tggcgcaaca actgcgccag ctgattcaac tcaatacgct gccggaactg gaacagcatc
     2641 gcgcacagcg tattctgcga gaaatcaatc aacatcatcg gcataaattc gcccatttcg
     2701 atgagaattt cgttgaacgt ctgctaaatc atccggaagt gccagaactg atccgcacca
     2761 gcccattgac ccaacgtgaa tggcaggtcc tggggctgat ttactccggt tacagcaatg
     2821 aacaaattgc gggcgaactg gaagtggcgg caaccaccat caagacgcat atccgcaatc
     2881 tgtaccaaaa actcggcgtc gcccaccgtc aggctgccgt gcagcacgcg cagaaactgc
     2941 tgaagatgat gggttatggg gtgtgagttt agccggataa tgcattggat ccggtttaca
     3001 tctttacatc attcaatact cacccgcgtt acgccatctg tttctatcaa accaaaccgc
     3061 accggcaaga aacgctccac caccgcgata ttggtcagca gatggcatga aggatgggcg
     3121 accttaaatt ctcccgcgcc cgccagcgcc atcggcagta ccagttggtc ggcgagatat
     3181 tcccccaccg ccgctgggct tgccaggtag cgtttcacct ctttcactaa ctgcgcggct
     3241 accacctcgg cactgacgcg cttttcaccg acgacgaaaa agcgttcagt gatattttca
     3301 ctttcgactt ccagcgagac ggtattaccc ggcccctggt cgcgcggcag gttatgaata
     3361 ttctgttcat gcagggaaaa actccccgcc agtgtagcga tttcacgctc agcaacatgg
     3421 cgcggcacac cagctaatag aacttctcca cgcatctgca caatgttccc gcgctcgcca
     3481 agttgcaagg tgttaaacga tgccacgggc gagacttccg ttgccaccac gccgcctccg
     3541 gcaggataaa aaccgtgacg taacagcgtg gtttgctgat gaattcctat tttcgccagc
     3601 agtggctcca gcacccggcg gataaaatcc gcaggcgggg ccgacgggtt atcggtgcct
     3661 ccgctcactt caacacgcga aggtccatcg gcaaaccaca gcgcgggcag caccgtttgc
     3721 agcaccagcg tacagcttcc ggcactaccg atagcaaagc ggtactcgcc gccgcgcacg
     3781 gtgccaggcc ggaagaccag acgctgcgac cccagctccg cgccttccac cgttgcccta
     3841 caaatttccg cagccgcttt taccgcggtc agatgctggc gcaacagccc cggtttcgcc
     3901 cgcccggcac gaatgctggt gatggtaaat ggctggccgg ttatcatcga caggctcagc
     3961 gccgagcgca ggatctgccc gccaccttcg ccctgtgcgc catccagcgc aatcatcctt
     4021 ttcatcattt atccttttac gcacaccacc tgacgcaggg tatagataac ttccaccaga
     4081 tcgctttgtg ccgccatcac cgcatcaata tctttatacg ccatcgggat ttcgtcgatc
     4141 acttcggcat ctttacggca ttccacatgc gcggtggcac gaatttgatc ttccacgctg
     4201 aacagttttt tcgctttagt acggctcatt acccgcccgg caccgtggct gcacgaacag
     4261 aacgactctt catttcccag cccacggacg ataaagcttt ttgctcccat cgaaccggga
     4321 ataattccat attgaccagc acgcgcagac accgcgcctt tacgcgtcac gtagatctct
     4381 tcaccaaagt gctgttcttt ttgcacatag ttgtggtgac agttgatctc ttccatcgcc
     4441 agcgtttgtg gctgtctgac cgttttctgc gtaatgctct gcaacgccgt taccacgttt
     4501 tccatcatcg catcgcggtt aaggctggcg aaaagctgcg cccaggccac ggctcttagg
     4561 taatcatcaa agtattccgt accttccata aagtacgcca ggtcgcgcga tggcaacgtt
     4621 tcaagctggt cctgcatctc tttttgtgcc aaatcgataa agtaagtccc gatggcgtta
     4681 ccaattccgc gtgaaccgga gtgcagcata atccacacct ggtctgactc atcaaggcag
     4741 atttcaataa agtggttacc cgttcccagc gttcccaggt gtttatagtt gttagtgtta
     4801 aggaaacgcg gatatttttg cgttaaccac tgataaccgg cttcaagctc agcccatttc
     4861 gcatcgacgt taacaggcgg gttttcccat gcgcctttat cacgtttaca acggccagtg
     4921 gtacgcccgt gcggcacggc catttcaatc gcctgacgca gctctgccag gttttcaggc
     4981 aggtcttccg ccgttaacgc ggtacgcagc gcgttcattc cacagccaat atccacgccc
     5041 accgccgccg gaataatcgc ccctttggtc gggatcacgc taccaatggt ggaaccttta
     5101 cccaggtgta catcaggcat taccgcaata tgtttgaaaa taaacggcat cttcgccgta
     5161 ttaataagtt gctgacgcgc atcggcttct accggcacgc ctttggtcca catttttacc
     5221 ggggcatttt cagtggtcag taattcgtaa ttcattttgt ttttctcttt tcgttgtttg
     5281 ctgt
//
LOCUS       DAZFRN010000254         5266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFRN010000254 DAZFRN010000000
VERSION     DAZFRN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.256"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..578
                     /locus_tag="ACHKUB_004888"
     CDS             <1..578
                     /locus_tag="ACHKUB_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6657782.1"
                     /translation="LKLNIHVFLRASELQFARWSEFDFKMRIWTIPAEREAIPGVRFS
                     ERGSKMKEEHLVPLSDQVMELLTCIKHISKESEFVLPGIRDKHKPMSENTINKALRTL
                     GYNTKLDVCAHGFRTMACSALNESEKWSKDAIERQMSHKDRNTVRAAYMHKAEFLEAR
                     IEMMQWWSDYLSLNQKEYIAPYIYARRSDVA"
     gene            1041..4634
                     /locus_tag="ACHKUB_004889"
     CDS             1041..4634
                     /locus_tag="ACHKUB_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO6657783.1"
                     /translation="MKNASKDILSAWQNYLKFSGAEKSKIPASQLHEHHQLMLGKDNC
                     EENQSGIVLHIAPEMHENWRRRFVKYGEKGEITHVEPVNLLFPVLRCIENERGKSSVK
                     YLPLFSFPLPKSFFEQNRASLLVPVKDGQQVSAFPFAFREIFGIQLEELGENRHMMSV
                     VSALTGCKYDTFLEAFEALQVWVIQHSNRKLETAFNALVAPLHNEDYNLKRDVEDFTW
                     LCGNAVGDFPLLEQYLNHIHSDETAPDIYEVVTGGLFETQYPLGHGQMAAIQAINKDE
                     RLIAVQGAPGTGKTTLFKSLIAQQLVSRALSIADGKDQNFGMLVTSTAIKAVENIIDD
                     LRNDPATQGLDWLWFHSGSNEQVQNELTRLEQLISRWHQESYNEQSQQDFLTVLLKHR
                     DELNSCYQTYLNEKATAIQSVVDCGLDTTNADELPARFMAKIPPVAAHAQRLGIDVSL
                     HHPDDLDNLTSLLEQRLQELRGIKAQRLQASRIYQTLQATWPQQHNLPQILRWMDSPL
                     RPALELHYRDYPRKGIKRHLARLFEGKYSARLQQMNLAAPEDFQHFSLSSLPHRQLAA
                     LADAGEILSHQTDNLALLELLMHDEPDGQQEENLVSLCADIATLRQQWQQIIRAQQVM
                     AQYQQHYPEGNWCDILRKRFIRQHREMFEAAIGYLWQEQLKRKIELEEVLTHWRALMS
                     NRRSAGYYRWLDKLDEFYRALSLVYPVMATTLVSAWKVAGYRKLDELKGHKPWHLALC
                     DEAGMISLESLVPLLSRSEKAMIVGDPLQIEPIRNLSENLQTQLRERHFADNNTLYER
                     VSPASVTGWHRAAGTLSGRVDDTGNGIILDEHRRCQKPIANLFIRLAGYHGVSVETTS
                     PSDRIASAFEKSGGHHLMFYSVEGQKGDMVNTNLDEVDAIEQLLNKLEEAGYDLTADV
                     GIVTPYSNQKSLLIKRLAQRMNHWQKNCIGTVHQFQGVGFEVIIYSPVIFHPLDNPNF
                     QNDTPNMLNVVVSRAKQQFIVVGNHHRLRQAGGYLKVLADSVAEDFLLEQGSQHPSFD
                     SLSQTPRLQRYYRDCEHIAAFSALASQCEQELVIITPWIRRGGKQYQRPELAQLVATQ
                     RRGVSVKVYYGYYHQRLDRAEDNDETLISEYREQLGKDNVIRLTEGTHEKVLMIDGRY
                     IVLGSWNWLSHGYHDGCERSEQFSNAIRHEISAELDDVSLVRELRERLRLE"
     gene            4707..>5266
                     /locus_tag="ACHKUB_004890"
     CDS             4707..>5266
                     /locus_tag="ACHKUB_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657784.1"
                     /translation="MATSSEQFRRFLESIKTAYIAFNKSLKKSHEENSNTFNFQPLTP
                     INDVNLDIYEDAISFVFSRPDIKNVAISGPYSAGKSSLIESYKTQYPSRKFSHISLSH
                     FISSTEQSDPNTKRLALEGKILNQIIHQISPEKIPRTKFKIKKKVFRRNVLTNTIFYL
                     SAGVSFTHIRLFDRWQSYVNTLNSGH"
BASE COUNT         1512 a         1195 c         1236 g         1323 t
ORIGIN      
        1 cactaaaact gaatatccat gttttccttc gggcaagcga gttacagttt gccagatgga
       61 gtgaatttga ctttaaaatg cgtatatgga ctattccagc cgaacgagaa gccatacctg
      121 gagtcagatt ttcggagcgc ggctcaaaaa tgaaagaaga gcatctggtt ccactttccg
      181 atcaggtgat ggagctactt acgtgcataa aacatataag taaagaatca gagtttgtat
      241 tacccggtat cagggacaaa cataagccca tgagcgaaaa tacaatcaac aaggcactcc
      301 gaaccctcgg atacaatacc aagcttgatg tttgtgcaca tggtttcagg acaatggcat
      361 gtagcgccct taacgaatcg gaaaaatggt caaaagatgc catagagcga caaatgagcc
      421 ataaagatcg caatacggtc agagcagctt atatgcataa ggccgaattt ttggaggccc
      481 gcatcgaaat gatgcagtgg tggtcagatt atcttagtct gaaccagaag gaatatattg
      541 ctccttacat ctatgcccgg cggtcagatg ttgcctgaaa aaaagcaaat taatcatccc
      601 gtaattttct gacaaaatcc cttccgaacg aacaagaaaa agtcaacaat cctacaagct
      661 atggggagtg taagaaacag agggcactgt ttacagtgcc ctcactcagt caatacataa
      721 acgctgttca gcatggtttg taagtttgtc agtaccataa tgttattctc aagccgttgg
      781 ccatttacag tcctcatact accgaatctg ccatgataca tatgaggccg ctttgtttat
      841 ctgttggttg cacattttat ctgtcagaca tgccagaaaa cgacacctgt acatgattga
      901 acaaccaagt tgaatgttgt aactaaaaac atgttttcat acagacaagg acagatggcc
      961 caggttaaaa ctttcacacc tgtggtattc tgttgtcatt aatttcaggg agttgaattg
     1021 atcaaggcac aatccgaata atgaaaaatg cgtctaaaga catcctctct gcgtggcaga
     1081 attacttgaa attcagcggt gcagaaaaga gtaaaatacc ggccagccaa ctgcatgagc
     1141 accatcagct catgcttggc aaagataact gtgaagaaaa ccagagcggt atagtgctcc
     1201 acattgcgcc ggaaatgcac gaaaactggc gcaggcgttt cgttaagtat ggtgaaaagg
     1261 gtgagattac ccatgttgag cccgtcaatc tgctttttcc ggtgctgcgt tgtatcgaaa
     1321 atgaacgtgg caaatccagt gtaaaatact tgccactgtt ctcttttcca ctgccaaagt
     1381 cattctttga acagaatcgc gcttctctgc tagttcccgt taaagacggg caacaggtca
     1441 gtgcattccc cttcgctttt cgcgaaatat tcggtatcca gctggaagag ttgggtgaga
     1501 accgtcatat gatgagtgtc gtcagcgctc ttaccggttg taaatatgac accttccttg
     1561 aggcatttga ggctcttcag gtatgggtta ttcagcattc aaatcgtaag ctggaaactg
     1621 cgtttaacgc cctggtagct ccactgcata atgaagatta caacctcaag cgtgatgtgg
     1681 aagattttac ctggttgtgc ggtaacgcag tcggtgactt ccctctgcta gagcagtatc
     1741 tcaaccatat tcacagtgac gagactgccc ctgacatata cgaagtggtg accggcggtt
     1801 tgtttgaaac acaataccca ctgggccatg gacagatggc ggcaatacag gccattaata
     1861 aggatgagcg cctgattgcc gttcagggcg caccgggtac tggcaaaacg acgttattta
     1921 agtccctcat cgcacaacag ttagtttcgc gagccctgtc gattgctgat ggcaaggatc
     1981 aaaacttcgg tatgctggtg acctcaacag caataaaagc ggtggaaaac attattgatg
     2041 atctgcgcaa tgatcccgca actcaaggtc ttgactggct ctggtttcat agcggttcca
     2101 atgagcaggt acaaaatgag cttacccgac ttgagcaact tatcagtcgc tggcaccagg
     2161 aaagttacaa cgagcaaagc cagcaagatt ttttaactgt tttactcaaa catcgcgacg
     2221 agttgaactc ctgttaccag acgtatctga atgaaaaggc aaccgcgata cagtctgttg
     2281 tggattgtgg tctggacact acaaacgcag atgagcttcc ggcacgtttt atggcaaaaa
     2341 tcccgccggt agccgctcat gcacaacgac tggggattga cgtttcactg catcatcctg
     2401 acgacctgga caacttaacc agcctgctgg aacaacgcct gcaggaactt cgcgggataa
     2461 aagcacagcg tctacaggcc agcagaattt atcagacatt gcaggcaaca tggccacagc
     2521 aacataatct gccgcagata ctgcgctgga tggattcacc actacgccct gctctggaac
     2581 tgcattaccg cgactatccg cgtaaaggga taaagcgtca tctggcccgc ctgttcgagg
     2641 gaaaatattc ggcacggctg caacaaatga atcttgctgc accggaggac tttcagcatt
     2701 tcagtctttc ttccctgccc catcgccagt tagcggctct ggctgatgcg ggagaaatac
     2761 tgtcacatca gacagacaac ctggccctgc tggaactgct catgcatgac gaacccgatg
     2821 ggcagcagga agaaaatctg gtgagcctgt gtgctgatat cgcaacgctc cggcaacaat
     2881 ggcagcagat catccgggca cagcaggtta tggctcagta tcaacagcat tatcctgaag
     2941 gtaactggtg tgacattttg cgtaaacgct tcatcaggca acaccgggaa atgtttgaag
     3001 ctgctatcgg ttatctctgg caggaacaac tgaagcgaaa aattgagctg gaagaggtgc
     3061 tgactcactg gcgggctctg atgagtaatc gccgcagtgc gggctactat cgctggctgg
     3121 ataagctgga tgaattttac cgcgctttgt cactggtgta tccggtaatg gcaaccacgt
     3181 tggtctccgc gtggaaagta gcgggctatc gtaagcttga tgaactcaag ggtcacaaac
     3241 cctggcacct ggcactgtgc gatgaagcgg gtatgatttc gcttgaatcg ctggtaccgc
     3301 tattgagtcg cagcgagaaa gcgatgattg tcggcgatcc cctgcaaatc gaacctatca
     3361 ggaatctgtc agaaaatctg caaacgcaac tgcgtgaacg tcatttcgct gacaataata
     3421 cattatatga gcgggtttca cctgcaagcg ttacaggctg gcaccgcgcg gctggtacgc
     3481 tttctggacg agtagatgat accggcaacg gtattattct tgatgagcat cgccggtgcc
     3541 agaaacccat tgccaatctc tttatccggc tagcgggcta tcatggcgtc agcgtggaaa
     3601 cgacctcccc ttccgaccgc attgccagcg cctttgagaa gtctggtggg catcatttga
     3661 tgttctacag cgtcgagggt cagaaaggcg atatggttaa cactaatctg gatgaagtcg
     3721 atgctattga gcaactgctg aataagctgg aagaagctgg ttacgatctg actgccgatg
     3781 tgggcatcgt cactccctac agcaatcaga aatcattact gatcaagcgc cttgcccagc
     3841 gtatgaatca ctggcagaaa aactgcattg gtacagtgca tcagttccag ggcgtgggat
     3901 ttgaggtcat catttacagc ccggtaattt ttcatcctct tgataatcca aattttcaga
     3961 acgatacgcc taacatgctg aatgtcgtgg tttcacgcgc aaaacagcag tttatagtgg
     4021 taggtaacca ccaccgattg cggcaggcag gtggatacct gaaagttctt gcggacagcg
     4081 ttgctgaaga tttccttctt gaacagggta gccagcatcc aagtttcgac agcctgagcc
     4141 agacaccgcg tctacaacgt tattaccgcg actgtgaaca tatcgccgca ttcagtgcgc
     4201 tggcgagcca gtgtgaacag gagctggtta ttatcactcc gtggatccgt cggggtggta
     4261 aacaatatca aagaccggaa ctggcgcaac tggtagcaac acagcggcgt ggtgtcagcg
     4321 ttaaagtcta ctacggttac taccatcaac gtctggaccg tgcagaggat aatgatgaaa
     4381 ctctgatttc tgagtaccgc gaacagcttg gaaaagacaa tgtcatccgc ctgaccgaag
     4441 gcactcatga gaaggtgctg atgatcgatg gccgttacat cgttctcgga agctggaact
     4501 ggctatctca tggttatcat gatggttgtg agcgtagcga gcaattcagt aatgctattc
     4561 gtcatgagat tagcgcggag ttggatgatg tgtcgctggt gagggaactt agggagaggt
     4621 tgcggctgga gtaacgccag aatcattttt agcattcatt tagttgttgt taaatatttt
     4681 atataattaa gttaataggt cagaaaatgg caacaagctc agaacaattc agacggtttt
     4741 tagaatcgat aaaaaccgca tacattgctt ttaataaaag cctaaaaaaa tcacatgagg
     4801 aaaatagcaa tacttttaac ttccaacctc ttacaccaat caatgacgtg aatcttgata
     4861 tttatgaaga tgctatttca ttcgtttttt cacgtccaga cattaagaat gttgccatat
     4921 caggcccata cagtgcaggc aaaagtagcc ttatcgaatc ctataagaca caatatccat
     4981 ccagaaaatt ctctcatatt tctttatctc attttatttc atcaaccgaa caaagcgatc
     5041 ctaatactaa gcgattagct cttgaaggaa aaatattaaa tcaaataatc caccagatct
     5101 caccagaaaa aatacccaga acaaaattca aaataaaaaa gaaagtattt cggagaaatg
     5161 tattaacaaa tacaattttt tacttatcag ctggcgtttc atttactcat atacgcttat
     5221 ttgataggtg gcaaagctat gtaaatacac taaatagcgg ccacat
//
LOCUS       DAZFRN010000255         5265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFRN010000255 DAZFRN010000000
VERSION     DAZFRN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.257"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            99..1433
                     /gene="garP"
                     /locus_tag="ACHKUB_004891"
     CDS             99..1433
                     /gene="garP"
                     /locus_tag="ACHKUB_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO6657785.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1449..2219
                     /gene="garL"
                     /locus_tag="ACHKUB_004892"
     CDS             1449..2219
                     /gene="garL"
                     /locus_tag="ACHKUB_004892"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO6657786.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2249..3139
                     /gene="garR"
                     /locus_tag="ACHKUB_004893"
     CDS             2249..3139
                     /gene="garR"
                     /locus_tag="ACHKUB_004893"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6657787.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3236..4381
                     /gene="garK"
                     /locus_tag="ACHKUB_004894"
     CDS             3236..4381
                     /gene="garK"
                     /locus_tag="ACHKUB_004894"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO6657788.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4414..4790
                     /gene="rnpB"
                     /locus_tag="ACHKUB_004895"
     ncRNA           4414..4790
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKUB_004895"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5066..>5265)
                     /locus_tag="ACHKUB_004896"
     CDS             complement(5066..>5265)
                     /locus_tag="ACHKUB_004896"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657789.1"
                     /translation="INTVDIYSSYPVHQKQKLTDAGLKLMATAKMPDALRLSGLRHLC
                     NILNLRAFVGRIRRSRRIRHE"
BASE COUNT         1240 a         1259 c         1500 g         1266 t
ORIGIN      
        1 aattcccgag ttcattgccg ttacggcata aatgaaactc ataaataaca ccttccccat
       61 aataataaaa atcagcataa gtacccgagg taaataaaat gattctggac accgttgacg
      121 aaaaaaagaa aggcgtgcat acccgctatt taatattact gattattttt attgttaccg
      181 ccgttaacta cgccgatcgt gcaacgctgt ctattgctgg taccgaagtg gcaaaagagt
      241 tgcagttaag tgcggtttcg atgggttaca tcttttctgc ttttggctgg gcctacttgc
      301 tgatgcaaat ccccggcggc tggctgcttg ataagtttgg ctcgaaaaaa gtttatacct
      361 atagcctctt tttctggtcg ctattcactt tcctgcaagg ctttgttgat atgttcccgc
      421 tggcctgggc agggatctcc atgttcttta tgcgctttat gctcggcttc tcggaagcgc
      481 catcattccc ggcgaacgcc cgtattgtcg ccgcctggtt cccgacgaaa gaacgtggta
      541 ctgcctccgc catctttaac tcggcgcaat atttctcgct ggcgctcttt tcgcctctgc
      601 ttggctggct gactttcgcc tggggctggg agcacgtctt taccgttatg ggggtgattg
      661 gttttgtgct gacggcgctg tggatcaaat tgattcataa cccgacagat cacccacgta
      721 tgtctgcgga agagctgaag tttatctctg aaaatggcgc ggtggtcgat atggaccaca
      781 aaaagccggg cagcgcggca gcaagcggtc ccaaactgca ttacatcaag caattgctct
      841 ctaaccgcat gatgttgggc gtatttttcg gacaatattt tatcaacacc atcacctggt
      901 tcttcctcac ctggttcccg atttatctgg tgcaggaaaa aggcatgtcg attctgaaag
      961 tgggtctggt cgcctcgatt ccggcactgt gtggttttgc gggcggcgtg ctgggaggtg
     1021 tcttctcgga ttatctgatc aaacgcggtt tatccctgac cctggcacgt aagctaccga
     1081 ttgtgctggg aatgttgctg gcttccacca tcatcttatg taactacacc aacaacacca
     1141 cgctggtggt catgctgatg gcgctggctt tttttggcaa aggatttggt gctctgggct
     1201 ggccggtgat ttctgacacc gcgccgaaag agattgttgg cctctgtggc ggcgtcttta
     1261 acgtctttgg caatgttgcc tccattgtca ctccactggt gattggctac ctggtaagtg
     1321 aactgcactc cttcaatgca gcactggttt tcgtgggatg ttcagcgctg atggcgatgg
     1381 tctgctacct cttcgtagtt ggcgacatta aacgtatgga attgcagaaa taagcaaagg
     1441 tataagcgat gaataacgat gttttcccga ataaattcaa agccgcactg gctgcgaaac
     1501 aggtacaaat tggttgctgg tcagcactct ctaacccgat tagcactgaa gttcttggtt
     1561 tggctgggtt tgactggctg gtgctggatg gcgaacatgc gccaaacgat atctccacgt
     1621 ttattccgca gttaatggcc ctgaaaggca gcgccagcgc gccagtagtg cgagtgccga
     1681 ccaacgagcc ggtaattatt aagcgtcttc tggatatcgg tttctataac ttcctgattc
     1741 cttttgtaga aacaaaagag gaagcagagc aggcggtggc atcaacccgt tacccaccgg
     1801 aaggcattcg cggcgtctcc gtttctcacc gcgccaatat gtttggcacc gtggcggatt
     1861 atttcgctca gtcgaacaag aacatcacca ttctggtcca gatagaaagt cagcagggcg
     1921 tagataacgt cgatgccatt gccgctaccg aaggtgtaga cggtatcttc gtcggcccca
     1981 gcgatctggc tgcggcatta ggccatctcg gcaatgcctc gcacccggat gtacaaaaag
     2041 caattcagca catttttaac cgtgccagtg cgcacggcaa acccagcggt attctcgcgc
     2101 cggtcgaagc cgatgcgcgt cgttatctgg aatggggcgc gacgtttgtg gctgtcggca
     2161 gcgatcttgg cgtcttccgc tctgccactc agaaactggc tgataccttt aaaaaataac
     2221 caccaccgca acaagagaga tgattgatat gactatgaaa gttggtttta ttggcctggg
     2281 gattatgggt aaaccaatga gtaaaaacct tctgaaagca ggttactcgc tggtggttgc
     2341 tgaccgtaac ccagaagcta ttgctgacgt gattgctgca ggtgcagaaa cagcgtctac
     2401 ggctaaagcg atcgctgaac agtgcgacgt catcataacc atgctgccaa actcccctca
     2461 tgtgaaagag gtggcgctgg gtgagaatgg catcattgaa ggcgcgaagc caggtacggt
     2521 attgatcgat atgagttcta tcgcaccgct ggcaagccgt gaaatcagcg aagcgctgaa
     2581 agcgaaaggc attgatatgc tggatgctcc ggtgagcggc ggtgaaccga aagccatcga
     2641 cggtacgctg tcagtgatgg tgggcggcga caaggctatt ttcgacaaat actatgattt
     2701 gatgaaagcg atggcgggtt ccgtggtgca taccggggaa atcggtgcag gtaacgtcac
     2761 caaactggca aatcaggtca ttgtggcgct gaatattgcc gcgatgtcag aagcgctaac
     2821 gctggcaacc aaagcgggcg ttaacccgga cctggtgtat caggcaattc gcggtggact
     2881 ggcgggcagt accgtgctgg atgccaaagc gccgatggtg atggaccgca acttcaagcc
     2941 gggcttccgt attgatctgc atattaagga tctggcaaat gcgctggata cttcccacgg
     3001 cgtcggcgcg caactgccgc tcacagctgc ggttatggag atgatgcagg cactgcgagc
     3061 agatggttta ggaacggcgg atcatagcgc cctggcgtgc tactacgaaa aactggcgaa
     3121 agtcgaagtt actcgttaat gacgtcgtgc ccggatggcg tattgcaatc cgggcctgga
     3181 atccaggccg aataagagaa acgccctccg gcgtcatgtg gtaacaggca taggtatgaa
     3241 aatcgtaatc gccccagact cttataaaga aagtttatct gccagcgagg ttgcgcaggc
     3301 gatagaaaaa ggatttcggg aaatttttcc tgatgcacag tacgtttcta ttccggttgc
     3361 cgacggtggc gaaggaacgg tggaagcgat gattgcagcc acccagggtt ctgaacgtca
     3421 cgcctgggtt acagggccgc tgggcgagaa agtgaatgcc agttggggga tctccggcga
     3481 tggcaaaacc gcgtttattg aaatggcggc ggccagtggg ctggagctgg tacctgcgga
     3541 aaaacgcgat ccactcgtga ccacttcacg cggcacaggc gagttaatcc tgcaggcgct
     3601 ggagagcggc gcgacaaaca ttattatcgg cattggcggt agcgctacaa atgatggcgg
     3661 cgcaggcatg gtacaggcgc tgggggcgaa attatgcgac gccaacggca atgaaattgg
     3721 ttttggcggc ggtagtctta atactctgaa tgatattgat atttccggcc tcgatccgcg
     3781 cttaaaagat tgcgtcattc gcgtcgcttg tgatgtcacc aatccgctgg tgggcgataa
     3841 cggcgcatcg cgcatctttg gcccacaaaa gggagccagt gaagcgatga ttgttgagct
     3901 ggacaataac ctctctcact atgccgatgt cattaaaaaa gcgctgcatg ttgatgtgaa
     3961 agatgtcccc ggcgcaggag ctgcgggcgg tatgggtgcg gcgctgatgg cgtttcttgg
     4021 tgcggaactg aaaagtggta ttgaaatcgt cactacggcg ctgaatctgg aggaacatat
     4081 tcacgattgt acgctggtga tcaccggtga agggcgtatt gacagccaga gtattcacgg
     4141 caaggtaccg attggcgttg caaacgtggc gaagaagtac cataaaccgg tgattggcat
     4201 tgcgggtagc ctgaccgatg atgttggcgt tgtacatcag catggcatcg atgcggtctt
     4261 cagcgtattg accagcatag gtacgttgga cgaagcattc cgcggggctt atgacaatat
     4321 ctgccgtgct tcacgtaata tcgccgcgac actggcgatt ggaatgcgca acgcggggtg
     4381 acaagggcgc gcaaaccctc tatactgcgc gccgaagctg accagacagt cgccgcttcg
     4441 tcgtcgtcct cttcggggga gacgggcgga ggggaggaaa gtccgggctc catagggcag
     4501 ggtgccaggt aacgcctggg ggggaaaccc acgaccagtg caacagagag caaaccgccg
     4561 atggcccgcg caagcgggat caggtaaggg tgaaagggtg cggtaagagc gcaccgcgcg
     4621 gctggtaaca gtccgtggca cggtaaactc cacccggagc aaggccaaat aggggttcat
     4681 aaggtacggc ccgtactgaa cccgggtagg ctgcttgagc cagtgagcga ttgctggcct
     4741 agatgaatga ctgtccacga cagaacccgg cttatcggtc agtttcacct gatttacgta
     4801 aaaacccgct tcggcgggtt tttgcttttg gaggagcgga aagatgaatg actgtccacg
     4861 acgctatacc caaaagaaag cggcttatcg gtcagtttca cctggtttac gtaaaaaacc
     4921 gcttcggcgg gtttttgctt ttggaggggc agaaagatga atgactgtcc acgacgctat
     4981 acccaaaaga aagcggctta tcggtcagtt tcacctgatg tacgtaataa accgttccgg
     5041 cgggtttcag attgttgagt gcgctttatt catgccggat gcggcgtgaa cgccttatcc
     5101 tgcctacaaa agcacgcaaa ttcaatatat tgcagagatg gcgtaggcct gataagcgta
     5161 gcgcatcagg catttttgcg gttgccatca gtttcaaacc cgcgtcagtg agtttttgct
     5221 tttggtgcac agggtaagat gaataaatat ctacggtatt aataa
//
LOCUS       DAZFRN010000256         5021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFRN010000256 DAZFRN010000000
VERSION     DAZFRN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.258"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..919
                     /locus_tag="ACHKUB_004897"
     CDS             <1..919
                     /locus_tag="ACHKUB_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO6657790.1"
                     /translation="RKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLT
                     PSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYITQGLESILGALDIEGSYD
                     KQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNM
                     FCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQR
                     VQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEE
                     QIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1128..2093
                     /gene="lipA"
                     /locus_tag="ACHKUB_004898"
     CDS             1128..2093
                     /gene="lipA"
                     /locus_tag="ACHKUB_004898"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO6657791.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2194..2397)
                     /gene="tatE"
                     /locus_tag="ACHKUB_004899"
     CDS             complement(2194..2397)
                     /gene="tatE"
                     /locus_tag="ACHKUB_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO6657792.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2526..3314)
                     /locus_tag="ACHKUB_004900"
     CDS             complement(2526..3314)
                     /locus_tag="ACHKUB_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO6657793.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3407..3790
                     /gene="crcB"
                     /locus_tag="ACHKUB_004901"
     CDS             3407..3790
                     /gene="crcB"
                     /locus_tag="ACHKUB_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO6657794.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3844..4053)
                     /gene="cspE"
                     /locus_tag="ACHKUB_004902"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3844..4053)
                     /gene="cspE"
                     /locus_tag="ACHKUB_004902"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6657795.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4228..4788)
                     /gene="pagP"
                     /locus_tag="ACHKUB_004903"
     CDS             complement(4228..4788)
                     /gene="pagP"
                     /locus_tag="ACHKUB_004903"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO6657796.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1349 a         1261 c         1117 g         1294 t
ORIGIN      
        1 cagaaagtcg tcagaaggta aaccacaaat atttacaacc ttacgaaata ttgatcttaa
       61 ccttctgact atttttgaag ctgtatatgt acataaaggg atcgttaatg cagcgaaagt
      121 gcttaatctg accccctcgg caatcagtca gtctattcag aaactgcgcg ttatattccc
      181 tgacccattg tttattcgca aaggccaggg tgtcactcct accgcatttg cgatgcatct
      241 acatgagtat atcactcagg gccttgagtc cattcttggc gcgctggata tcgaaggaag
      301 ctatgataag caacgaacga taactattgc gaccactccc tcggtcggag cactggtcct
      361 tcctgtcatc tatcgggcga ttaaaactca ctatccgcag cttttactgc gcaacccgcc
      421 catcagcgac gcggaaaacc aactcagtca gtttcaaacc gatctcatca tcgataacat
      481 gttttgcacc aatcgtacgg tgcaacatca tgttctgttc accgacaata tggtgttaat
      541 ttgccgcgag ggaaatccac tactctcttt agaagatgac agagagacta tcgacaacgc
      601 tgcgcatgta cttctgttac cggaagagca aaatttcagc ggtctgcggc agagagttca
      661 agaaatgttt ccggaccggc aaatcaattt caccagctac aacattttga caatcgctgc
      721 actggttgcc aacagtgaca tgttagcgat tattcccagc cgtttttata acctgtttag
      781 ccgctgctgg ccgctggaaa aactgccttt tccgtcctta aatgaggagc aaatagattt
      841 ctctatccac tacaacaaat tcagcctgcg tgatcccatc ctgcacggag taatagatgt
      901 tatccgtaat gcattttgaa aaagtattgt tacagccaga tcacagcgca gaacgataaa
      961 atggcacaat ccaacaacaa ttttacattt tcgcgaccgc tttggctgct ttcaggtccg
     1021 tttcaatgat atactgccag tcgttaattc aaaaatagtt gataattaca acaatctatt
     1081 gaattgaaac gctttccttc gtaattcgca actggaacac gcacgctatg agtaaaccca
     1141 ttgtgatgga acgcggtgtt aaataccgcg atgccgataa gatggccctt atcccggtta
     1201 aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc ggaatggatg aaaatcaagc
     1261 ttccagcgga ctctacacgt atccagggca tcaaagccgc aatgcgcaaa aatggcctgc
     1321 attctgtctg cgaggaagcc tcctgcccta acctggcgga atgcttcaac cacggcacag
     1381 caacgtttat gatcctcggc gctatttgta cccgccgttg tccgttctgt gatgttgccc
     1441 acggtcgccc ggtagctcct gatgccaatg aaccagtgaa actggcgcag accattgccg
     1501 atatggcgct gcgttatgtg gttatcacct ccgttgaccg tgatgacctg cgcgatggcg
     1561 gtgcccagca ctttgcggat tgcattactg ccattcggga aaaaagcccg caaatcaaaa
     1621 ttgaaactct ggtgccggat ttccgtggtc gtatggatcg tgctctggat attctgactg
     1681 caacgccacc agatgtgttc aaccataacc tggaaaacgt accgcgtatt taccgtcagg
     1741 tacggcctgg tgcagattac aactggtcgc tgaagctgct ggaacgcttt aaagaagcgc
     1801 atccggaaat cccgaccaag tctggtctga tggtgggact gggtgaaacc aatgaagaaa
     1861 ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac gatgttaacg ctggggcaat
     1921 atttgcagcc aagccgccat cacctgccgg ttcaacgtta cgttagcccg gatgagttcg
     1981 acgaaatgaa agccgaagcg ctggcgatgg gctttaccca tgctgcatgc ggtccgtttg
     2041 tccgctcttc ttaccacgcc gatttgcagg cgaaagggat ggaagttaag taatcctgta
     2101 ataattactt acatctgtct gataaaaaac ccgctttttg aagcgggttt tttgtatcga
     2161 acaagatatt gagggagcgt cctgctcgcc acgtcactct ttatgagaga gcttttcagc
     2221 ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg tcatcattca tcgccttctt
     2281 gaacccttta atggccgctc caaggtctcc gcccagcgta cgtaacttct tagtcccaaa
     2341 cagcagaacg accagcgccg caactaccag cagtttggta atactaatct cacccataga
     2401 taccttcttg acatataacc ggcaacagaa cgcgccttaa aaaccagaga atattaacgg
     2461 tcattcgacg cgcgcacaca atagcaatct gtaacaaggt gaatcaagcg ccgagtagaa
     2521 aaacatcata ataattgcgg cggcgcaaag cgacggttgt ttaagacggg cagttgcgcg
     2581 cgcacctgac gcacacgttc gggcgtcact tccgccataa tgagtgcagg catttctgac
     2641 gctgccgcaa tggtgacgcc aaagggatct ataatccggc tttgaccgat atttttgttc
     2701 ccgcactccc ccgccgccac catataacag gtggtatcca gcgcacgagc ggcaagcaac
     2761 gttgaccagt gatgctcttt gagcggcccg cgaacccagg cggcaggaag taccaggatt
     2821 tcagctccct gtaatgcctg tgccagcgcc agctctggaa agcgtaagtc ataacaggtc
     2881 atcagaccga ccttcatccc ctccacctcg agtaacggag cgatttcatt accagcatca
     2941 acacggcgtg attcctgaat ggcaaatgca tcatagagat gcaatttggc ataacgggcg
     3001 acgatgtttc ctgcctgtag cgccaccagc atattccatg cgcgccccgg cgttgaagga
     3061 acatgaatcg tcagaattgt cgtcatcatg ttacgtttac tttctcgccg taaacgtccg
     3121 aggaattcgc cttccagcag ctgtgctgat ttaaccgata gatctgcatc atgatcatcg
     3181 cgcgccagca atgcttccgg caggacgaac agcgatacgt cgttttccgc cgcctgcgcc
     3241 atcaacgagg cacaaatctc agcgttcttt tcccacacag atgtaacagc aaactgtcct
     3301 gcggcaacca gcattgccac ctcctgtcag agtcgtaaaa ttcgccaacg acgattattc
     3361 ggcgttacac ttatcactca tacaaatcaa atagcaggat tttgcagtgt tacaacttct
     3421 tttagcagtt tttattggcg gtggtacggg aagcgtggcg agatggctgt taagtatgcg
     3481 atttaacccg ctgcatcagg cgattccgtt ggggacgctg gcagcaaacc tgattggggc
     3541 attcatcata ggaatgggat tcgcctggtt cagcaggatg acgaacattg atccagtgtg
     3601 gaaagtatta atcaccaccg gattttgtgg cggtctaaca accttctcaa cattttcggc
     3661 agaagtggtg tttttgttac aagagggccg ctttggctgg gcgttactga acgttttcgt
     3721 caaccttctg gggtcttttg ccatgacagc actggctttc tggctgtttt cggcctcaac
     3781 cgcacactaa agaccaaaaa aaacccgctg attaagcggg ttttgaattc ttgctgacgt
     3841 atcttacaga gcgattacgt ttgcagcaga agggcctttg gcaccgttag tgatttcgaa
     3901 ctctacgcgc tgaccttcag caagagtttt aaaaccatta gtctggattg cagagaagtg
     3961 tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa ccgaatcctt tggactcatt
     4021 aaaccactta acgttacctt taatcttaga catcaaaatt acctttacat gaaaaataga
     4081 cacaaatgct gtgtcggtta ccagtacacc aattgtggta cgctttgtcc agtgcaactt
     4141 tactaaaaag tgataaaagt cgcgttattt ttgatgttac ttattttagc tattgatttt
     4201 aaagaagtta ctaaaacttc atttgtctca aaactgaaag cgcatccagg caaagtacac
     4261 attgccattg ttgtaggtac ccggaatgta ggtcatctga aaagtcactg ggccataacc
     4321 cacggaggcc aatggcagta gaaccgggag agggatgtaa ttccagttat cgcgtgccgt
     4381 tacgccagcg gtgaatccca gacctaaatg aaaattttca tccgccagcg gtcgccaggt
     4441 actttcccat ccgtatccgg caatcggttc ccatttgttc cacgagtcct taaatgccat
     4501 ggcatacagg ccatgccagt ttcctttttc atcccaacgc gacaggccaa aaccgccacc
     4561 ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg taagcgaaac gtgcatgcca
     4621 ggtgatggca ggaatatata aatcataatg ttcaggctgt tgccaggttt gtgcaatatt
     4681 ttctctaaac gttgtcatcc actcatctgc gttagcaaaa actttaccaa cgctgattaa
     4741 ctgaataaaa acaaaggaaa agatagcgac atatttactc acgttcattt gtgaccataa
     4801 aacatttatc aaaaatctac tactagcata gcaaagctac aattaacata accttaatag
     4861 acccaacata aagaataatc tgaatagctg ctctatttat ttccgcttgt tcaagttaaa
     4921 tatttccgcg catcgattaa agatgatgcg tcgttttctg cgatgggaat agtcaaaaaa
     4981 gaaaaaccaa gtcttttttg atgacaaaat gcaatcaagg a
//
LOCUS       DAZFRN010000257         4936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFRN010000257 DAZFRN010000000
VERSION     DAZFRN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.259"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..668
                     /gene="fimC"
                     /locus_tag="ACHKUB_004904"
     CDS             <1..668
                     /gene="fimC"
                     /locus_tag="ACHKUB_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6657797.1"
                     /translation="LIIFYLIISASAHAAGGIALGATRIIYPADAKQTAVWIRNSHTN
                     ERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNV
                     KTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVHN
                     PSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDYGSVTPVREV
                     NLN"
     gene            699..3308
                     /gene="sfmD"
                     /locus_tag="ACHKUB_004905"
                     /pseudo
     CDS             699..3308
                     /gene="sfmD"
                     /locus_tag="ACHKUB_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3321..4328
                     /gene="fimH"
                     /locus_tag="ACHKUB_004906"
     CDS             3321..4328
                     /gene="fimH"
                     /locus_tag="ACHKUB_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6657798.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRARPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            4339..4854
                     /gene="sfmF"
                     /locus_tag="ACHKUB_004907"
     CDS             4339..4854
                     /gene="sfmF"
                     /locus_tag="ACHKUB_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO6657799.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(4857..>4936)
                     /locus_tag="ACHKUB_004908"
     CDS             complement(4857..>4936)
                     /locus_tag="ACHKUB_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="activates the production of the major fimbrae
                     protein FimA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbriae biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO6657800.1"
                     /translation="SNIYGKLGLHSIVELIDYAKLYELI"
BASE COUNT         1279 a         1083 c         1209 g         1365 t
ORIGIN      
        1 tgctcattat attttattta atcatttcgg ccagcgccca tgctgccgga gggatcgcat
       61 taggtgccac gcgtattatt tatcccgctg atgctaaaca gactgcggta tggattagaa
      121 atagccatac caatgagcgc tttctggtca attcgtggat tgaaaacagc agcggtgtaa
      181 aagaaaagtc attcatcatt acaccgccac tgtttgttag tgaacccaaa agcgaaaata
      241 ccttgcgtat tatttacacc ggtccaccgc tggcggcaga tcgtgagtct ctgttctgga
      301 tgaatgttaa gacgatccct tcggtagata aaaatgcatt gaacggcagg aatgttttgc
      361 aactggcgat tttatcgcgc atgaaattat ttctccgtcc aattcaatta caagaattac
      421 ccgcagaagc gccggacaca ctcaagtttt cgcgatccgg taactatatc aatgttcata
      481 atccatcacc tttttatgtc accctcgtta acctgcaagt gggcagccaa aagttgggga
      541 atgctatggc ggcacccaga gttagttcac aaattccctt accctcagga gtgcagggaa
      601 agctgaaatt tcagaccgtt aatgattatg gttcagtaac cccggtcaga gaagtgaact
      661 taaactaacc gaatcatctg acaatatcag agctaattat gaaaataccc actactacgg
      721 atattccgca gaggtatacc tggtgtctgg ccggaatttg ttattcatct cttgccattt
      781 taccctcctt tttaagctat gcggaaagtt atttcaaccc ggcattttta ttagagaatg
      841 gcacatccgt tgctgattta tcgcgctttg agagaggtaa tcatcaacct gcgcgcgtgt
      901 atcgggtgga tctctggcgt aatgatgagt tcattggtta acaggatatc gtatttgaat
      961 cgacaacaga aaatacaggt gataaatcag gtgggttaat gccctgtttt aaccaggtac
     1021 tccttgaacg aattggcctt aatagcagtg catttcccga gttagcccag cagcaaaaca
     1081 ataaatgcat caatttactg aaagctgtac ctgatgccac aattaacttt gattttgcag
     1141 cgatgcgcct gaacatcact attcctcaga tagcgttgtt gagtagcgct cacggttaca
     1201 ttccgcctga agagtgggat gaaggtattc ctgctttact cctgaattat aatttcaccg
     1261 gtaacagagg taatggtaac gatagctatt tttttagtga gttcagcggg attaatattg
     1321 gcccgtggcg tttacgcaac aatggttcct ggaactattt tcgcggaaat ggatatcatt
     1381 cagaacagtg gaataatatt ggcacctggg tacagcgcgc cattattccg ctgaaaagtg
     1441 aactggtaat gggagacggc aatacaggaa gtgatatttt cgatggtgtt ggatttcgtg
     1501 gtatacggct ttactcttcc gataatatgt atcctgatag ccagcaaggg tttgccccaa
     1561 cagtacgtgg gattgcccgt acggcggccc agctaacgat tcggcaaaat ggttttatta
     1621 tctatcaaag ctatgtttcc cccggcgctt ttgaaattac agatttgcac ccgacatctt
     1681 caaatggcga tctggacgtc accatcgacg agcgcgatgg taatcagcag aattacacaa
     1741 ttccgtattc aacagtgcca attttacaac gcgaagggcg tttcaaattt gacctgacgg
     1801 cgggcgattt tcgtagcggt aatagtcagc aatcatcgcc tttctttttt cagggtacgg
     1861 cactcggcgg tttaccacag gaatttactg cctacggcgg gacgcaatta tctgccaatt
     1921 acaccgcctt tttattaggg ctggggcgca acctcgggaa ctggggcaca gtgtcgctgg
     1981 atgtgaccca tgcgcgcagt cagttagccg acgacagtcg tcatgagggg gattccattc
     2041 gcttcctcta tgcgaaatcg atgaacacct tcggcaccaa ttttcagtta atgggttacc
     2101 gctattcgac acaaggtttt tatacccttg atgatgttgc gtatcgtcga atggaggggt
     2161 acgaatatga ttacgattac gacggtgagc atcgggatga accgataatc gtgaattacc
     2221 acaatttacg ctttagccgt aaagaccgtt tgcagttaaa tatttcacaa tcacttaatg
     2281 actttggctc gctttatatc tctggtaccc atcaaaaata ctggaatact tcggattcag
     2341 atacctggta tcaggtgggg tataccagca gctgggttgg catcagttat tcactctcat
     2401 tttcgtggaa tgaatctgta gggatccccg ataacgaacg tattgtcgga cttaatgttt
     2461 cagtgccttt caatgttctg accaaacgtc gctacacccg ggaaaatgcg ctcgaccgcg
     2521 cttatgcctc ctttaacgcc aaccgtaaca gcaacgggca aaatagctgg ctggcaggtg
     2581 taggtgggac cttactggaa ggccacaacc tgagttatca cgtaagccag ggtgatacct
     2641 cgaataatgg gtatacgggc agcgccacgg caaactggca ggccacttac ggtacgctgg
     2701 gggtcgggta taactacgac cgcgatcaat atgacgttaa ctggcagctg tctggcggtg
     2761 tggtcgggca cgaaaatggc ataacgctga gccagccttt aggggatacc aatgttttga
     2821 ttaaagcgcc tggcgcaggc ggtgtacgca ttgaaaatca aactggcatt ttaaccgact
     2881 ggcgcggcta tgcggtgatg ccgtatgcca cggtttatcg gtataaccgt atcgcgcttg
     2941 ataccaatac gatggggaat tccatcgatg ttgaaaaaaa tattagcagc gttgtgccga
     3001 cgcaaggcgc gttggttcgt gccaattttg atacccgcat aggcgtgcgg gcgctcatta
     3061 ccgttaccca gggcggaaaa ccggtgccgt ttggatcact ggtacgggaa aacagtaccg
     3121 gaataactag tatggtgggt gatgacgggc aagtttattt aagtggtgcg ccattgtctg
     3181 gtgaattact ggttcagtgg ggagacggcg cgaactcacg ctgcattgcg cactatgtat
     3241 tgccgaagca aagcttacag caagccgtca ctgttatttc ggcagtttgc acacatcctg
     3301 gctcataaag gaaattatca atgaagataa tctgcagatt attattggcg atggcatgtt
     3361 tgtgtctggc aaacatatcc tgggctactg tttgtgcaaa tagtactggc gtagcagaag
     3421 atgaacacta tgatctctca aatatcttta atagcaccaa taaccagcca gggcagattg
     3481 ttgttttacc ggaaaaatcc ggctgggtag gtgtctcagc aatttgtcca cccggtacgc
     3541 tggtgaatta tacataccgt agttatgtca ccaactttat tgttcaggaa actatcgata
     3601 attataaata tatgcaatta aatgattatc tattaggtgc gatgagtctg gttgatagtg
     3661 tgatggatat tcagttcccc ccgcaaaatt atattctgat gggaacagat cctaacgttt
     3721 cgcaaaacct tccattcggg gtgatggatt ctcgtttaat atttcgttta aaggttattc
     3781 gtccctttat taacatggtg gagatcccca gacaggtgat gtttaccgtg tatgtgacat
     3841 caacgcctta cgatccgttg gttacacctg tttataccat tagttttggt ggccgggttg
     3901 aagtaccgca aaactgcgaa ttaaatgccg ggcagattgt tgaatttgat tttggtgata
     3961 tcggcgcatc gttatttagt gcggcagggc cgggtaatcg acctgctggt gtcatgccgc
     4021 aaaccaagag cattgcggtc aaatgtacga atgttgctgc gcaggcttat ttaacaatgc
     4081 gtctggaagc cagtgccgtt tctggtcagg cgatggtgtc ggacaatcag gatttaggtt
     4141 ttattgtcgc cgatcagaac gatacgccga tcacgcctaa cgatctcaat agcgttattc
     4201 ctttccgtct ggatgcagct gcggcagcca atgtcacact tcgcgccagg cctatcagta
     4261 ttaccggtca aaaaccgacc gaagggccgt ttagcgcgct ggggtattta cgcgtcgatt
     4321 atcaatgagg tacggagaat gagaagagca ctctttagct gtttctgcgg gttactgtgg
     4381 atttccagtg gatgggcagc tgaccctttg ggaacgatta atatcaattt gcacggtaac
     4441 gttgttgatt tctcctgtac cgtaaacact gcggatattg ataagacggt agatttaggc
     4501 agatggccta cgacacaatt actgaacgct ggcgatacca cggcactcgt cccttttagc
     4561 ctgcggctgg agggatgtcc tccgggttca gttgcgattt tatttacggg aacgccggca
     4621 tccgatacca acctgctggc tctggatgat cccgcaatgg cgcaaaccat cgccatcgaa
     4681 ttacgtaata gcgatcgctc ccggctcgca ctgggagcgg cgagcccgac tgaggaagta
     4741 gatgcaaatg gcaatgtcac actaaacttt tttgccaatt atcgagcgtt agccagcggt
     4801 gttcggccag gtgtggcgaa agcggatgcg atatttatga tcaattataa ttaatattat
     4861 attaattcgt ataatttggc gtagtcgata agctctacaa ttgaatgcaa acctagcttg
     4921 ccataaatat tagatt
//
LOCUS       DAZFRN010000258         4928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFRN010000258 DAZFRN010000000
VERSION     DAZFRN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.260"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            67..666
                     /locus_tag="ACHKUB_004909"
     CDS             67..666
                     /locus_tag="ACHKUB_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFO6657801.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            731..3646
                     /locus_tag="ACHKUB_004910"
     CDS             731..3646
                     /locus_tag="ACHKUB_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6657802.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFEQMVEEVARNASVVAQNTAAAKKSASDAGTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEAEKSAAAAESSKSAAATSAAAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEASRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLATAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHS
                     VSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            3646..4221
                     /locus_tag="ACHKUB_004911"
     CDS             3646..4221
                     /locus_tag="ACHKUB_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6657803.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSSY
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENITWLSP
                     EGEFQKWNGTAWVKDTEAAKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(4319..4909)
                     /locus_tag="ACHKUB_004912"
     CDS             complement(4319..4909)
                     /locus_tag="ACHKUB_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6657804.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT         1277 a         1240 c         1347 g         1064 t
ORIGIN      
        1 attttataac ccagaacggg cgtcagaaat gacgcctttt ttattgcaga aaagcgagag
       61 gtaattatgc gtaaacttta tgccgccatt ttgtccgcag ccatttgtct ggccgtatcc
      121 ggtgcgcctg catgggcgtc tgaacatcag tccacgctga gcgcggggta tcttcatgcc
      181 cggacgaacg ttcccggcag tgatgatctg aacgggatta acgtgaaata ccgttatgag
      241 tttacggaca cactggggct ggtgacgtca ttcagctatg caggagacaa gaatcgccag
      301 cttacccgtt acagcgatac ccgctggcat gaagattccg tgcgtaaccg ctggttcagc
      361 gtgatggtgg ggccgtctgt gcgcgtgaat gaatggttca gcgcgtatgc gatggcgggt
      421 atggcttaca gccgtgtgtc gactttctcc ggggattatc tccgcgtaac tgacaacaag
      481 gggaaaacgc acgacgtgct gaccggaagt gatgacggtc gccacagcaa cacgtctctg
      541 gcgtggggag ctggcgtgca gtttaacccg accgaatccg tggccattga tattgcttat
      601 gaaggctccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt cggttataag
      661 ttctgattag ccaggtaaca cagtgttatg acagcccgcc ggttcaggcg ggcttttttg
      721 tggggtgaat atggcagtaa agatttcagg tgtactgaaa gacggcacag gaaaaccggt
      781 acagaactgc acaatccagc tgaaagcaaa acgtaacagc accacggtgg tggtgaacac
      841 gctggcctca gaaaatccgg atgaagccgg gcgttacagc atggacgttg agtacgggca
      901 gtacagcgtt attctgttgg tggaaggatt cccgccgtca catgccggga ccatcaccgt
      961 gtatgaagat tctcaacccg gtacgctgaa tgattttctc ggtgccatga cggaggatga
     1021 tgcccgtccg gaggcactgc gccgttttga acagatggtg gaagaggtgg cgcgtaacgc
     1081 gtccgtggtg gcacagaaca cggcagccgc gaagaagtca gccagtgatg ccggcacatc
     1141 tgcccgtgag gcggcaaccc atgcgactga tgctgcagat tcagcacgtg cagccagcac
     1201 gtcagccgga caggccgctt cgtcggctca gtcagcgtct tccagcgcag gaacggcatc
     1261 aacaaaggcc actgaagcgg aaaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc
     1321 agctaccagt gccgctgccg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc
     1381 agccgccact tctgcatcca ccgcgactac gaaagcgtca gaagctgcca cctcagcccg
     1441 ggatgcgtcg gcttcaaaag aggcggcaaa atcatcagaa acgaacgcat cctcgagcgc
     1501 cagtagcgca gcttcctcgg caacggcggc agcaaattcc gcgaaggcgg caaaaacgtc
     1561 cgagacgaac gccaggtctt ctgagacggc agcgggacag agcgcctcag ctgcggcagg
     1621 ctcaaaaaca gcggctgcgt cgtctgccag tgccgcgtca acaagtgccg ggcaggcctc
     1681 agccagtgcc accgctgccg gaaaatcggc agaaagcgcc gcatcgtccg cttcaacagc
     1741 cacaacgaag gctggcgaag ccactgaaca ggccagcgca gcagcgaggt ctgcttccgc
     1801 agcgaagaca tccgagacga acgcgaaagc gtcggaaacc agcgcagaat cctcaaaaac
     1861 ggctgccgca tcgtccgcca gttcggcggc gtcatcggca tcatctgcgt ctgcttcaaa
     1921 agatgaggcg agcagacagg cgtcagcagc aaagggcagc gccacgacgg catccacgaa
     1981 ggcgacagag gcagctggca gtgcgacggc ggcagctcag agcaaaagta cggcggaatc
     2041 cgcggcaacg cgcgccgaga cagcggcaaa acgggcagag gatattgcat ccgccgtggc
     2101 gcttgaggat gcgagcacga cgaaaaaggg gatagtacag ctcagcagtg cgaccaacag
     2161 cacttccgag tcactggcgg caacgccaaa agccgttaag gccgcgtatg agctggctaa
     2221 cgggaaatac accgcacagg atgcaacgac agcacagaaa gggatagttc agctcagcag
     2281 cgcgaccaac agcacatctg aaatgctggc ggcaacgcca aagtcggtaa aggcagccta
     2341 tgaccttgct aacgggaaat atactgctca ggacgctacg acagcacaaa aaggaattgt
     2401 ccagctcagt agtgcaacca acagcgcatc tgaaacgctt gccgcgacac cgaaagcagt
     2461 gaaagcagct aatgataatg cgaatggtcg ggtaccttct gcccgtaagg tgaatggtaa
     2521 ggcgctttca gcggatataa cactgacgcc gaaagatatt ggtacgctta actcaacaac
     2581 aatgtcattc agcggtggtg ctggttggtt caaattagca acggtaacca tgccacaggc
     2641 gagttctgtt gtttcaatta cgttgattgg tggcgcggga tttaacgtgg ggtcacctca
     2701 acaggcaggt atatctgaac ttgttttgcg tgcaggtaat ggtaatccga aggggattac
     2761 tggtgcttta tggcagcgca catcgacagg gtttacaaat tttgcctggg tcaatacatc
     2821 tggtgatact tacgatattt acgttgcaat cggaaattat gcgactggtg taaatattca
     2881 atgggattat accagtaatg ccagcgtgac tattcatacg tcaccagcat attctgctaa
     2941 taagccggaa gggttaacgg acggtacagt ttattcactc tatacgccat cagagcagtt
     3001 ttatccgcct ggcgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
     3061 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcaaccg cttatccgtc
     3121 aggcgtgatc cctgatatgc gtggctggac gattaagggc aaacctgcca gtggtcgggc
     3181 cgtattgtct caggaacagg acggcattaa atcgcacacc cacagcgcca gcgcatccag
     3241 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
     3301 caccggggcg catacgcaca gtgtgagcgg tacagccgca agtgccggaa accatactca
     3361 tagtgtcaca ggcgcatcag cagtcagcca gtggtcacaa aatgggtcag tacataaggt
     3421 agtgtctgcg gccagtgtga atacaagtgc tgcaggagcg cacactcata gtgtcagcgg
     3481 cacagctgca tctgcaggtg ctcacgcaca tactgtcggt attggtgctc atacgcactc
     3541 tgttgcgatt ggctcacatg gacacaccat caccgttaac gctgctggta acgcggaaaa
     3601 cactgtcaaa aacatcgcat ttaactacat tgtgaggctt gcataatggc attcagaatg
     3661 agtgaacaac cacggaccat aaaaatttat aatctgctgg caggaactaa tgaatttatt
     3721 ggtgaaggtg acgcatatat tccgcctcat acaggtctgc cagcaaacag ttcctatatt
     3781 gcaccgccag atattccggc tggcttcgtg gctgttttca acagtgatga ggcatcgtgg
     3841 catctcgttg aagatcatcg gggtaaaacc gtctatgacg tggcttccgg cgacgcgtta
     3901 tttatttctg aacttggccc attaccggaa aatatcacct ggttatcccc ggaaggggag
     3961 tttcagaagt ggaacggcac agcctgggtg aaggatacgg aagcagcaaa actgttccgg
     4021 atccgggagg cggaagaaac aaaaaacaac ctgatgcagg tagccagtga gcatattgcg
     4081 ccgcttcagg atgctgcaga tctggaaatt gcaacggagg aagaaatctc gttgctggaa
     4141 gcatggaaaa agtatcgggt attgctgaac cgtgttgata cgtcaactgc acaggatatt
     4201 gaatggccag cactgccgta gggtaaaaca tataaattct ataattagat gtatctttcc
     4261 atttacggca aggaaggggg cttggaagac gtaaagcatc tcacaccgag attatttttt
     4321 atatgtcagg tgtctgaagt tttgctttgg ctcttaaaat ggtttgccgc gaggttttga
     4381 attcccgggc aatggcactt atacttacac ctgacttaat tcgttcgaat accacctgtt
     4441 tctgttcttc atttaacaca ggtggtcgac caaaacgttt ccctgcgccg cgggctctta
     4501 ctatcccgga atgagtgcgt tcaagtaaaa ggtctcgttc aaattcagcg actgctgaaa
     4561 ttacttgcat catcattttt cctgttggac tggtcaggtc aatgcccccc aatgctaagc
     4621 aatgcactct gatacctgtt tcggtcagtt gttccactgt tttcctgata tccattgcat
     4681 tacaaccaag gcgatccagt tttgtcacaa tcaattgatc accacatttc aggcgagcaa
     4741 gcaaccggtt aaaaccagga cgctcactgg ttgctgctga gccgctaatg tgttcttcga
     4801 ttatttgctg aggtttgatt ttaaaacctg cactttcgat ttcccggcgt tgattttcgg
     4861 tggtctgatc cagcgttgat atccgacagt aagcaaaaat tcgagacata gtgagattct
     4921 atacgaaa
//
LOCUS       DAZFRN010000259         4905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFRN010000259 DAZFRN010000000
VERSION     DAZFRN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.261"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            174..1118
                     /gene="leuO"
                     /locus_tag="ACHKUB_004913"
     CDS             174..1118
                     /gene="leuO"
                     /locus_tag="ACHKUB_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO6657805.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLER
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1273..1317)
                     /locus_tag="ACHKUB_004914"
     CDS             complement(1273..1317)
                     /locus_tag="ACHKUB_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657806.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1436..3160
                     /gene="ilvI"
                     /locus_tag="ACHKUB_004915"
     CDS             1436..3160
                     /gene="ilvI"
                     /locus_tag="ACHKUB_004915"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO6657807.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMAFPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3163..3654
                     /gene="ilvN"
                     /locus_tag="ACHKUB_004916"
     CDS             3163..3654
                     /gene="ilvN"
                     /locus_tag="ACHKUB_004916"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6657808.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3666..3752
                     /locus_tag="ACHKUB_004917"
     CDS             3666..3752
                     /locus_tag="ACHKUB_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO6657809.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3834..4838
                     /gene="cra"
                     /locus_tag="ACHKUB_004918"
     CDS             3834..4838
                     /gene="cra"
                     /locus_tag="ACHKUB_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO6657810.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1185 a         1126 c         1300 g         1294 t
ORIGIN      
        1 ctcattccac ggcaatggat tctgttttta tcagaacccg tatctttatg ttttccgaat
       61 tttactcatt ttgctttttc ttattttata tgcatgataa atcatattct tcaggattat
      121 ttctctgcat tccaataagg gaaagggagt taagtgtgac agtggagtta agtatgccag
      181 aggtacaaac agatcattca gagacggcgg agttaagcaa gccacagcta cgcatggtcg
      241 atctcaactt attaaccgtt ttcgatgccg tgatgcagga gcaaaacatt acccgtgccg
      301 ctcatgttct gggtatgtcg caacctgcgg tcagtaacgc tgttgcacgc ctgaaggtga
      361 tgtttaatga cgagcttttt gttcgttatg gccgtggtat tcaaccgact gctcgcgcat
      421 ttcaactttt tggctcagtt cgtcaggcat tgcaactagt acaaaatgaa ttgcctggtt
      481 caggttttga acccgcgagc agtgaacgtg tatttcatct ttgtgtttgc agcccgttag
      541 acagcattct gacctcgcag atttataatc acattgagca gattgcgcca aatatacatg
      601 ttatgttcaa gtcttcatta aatcagaaca ctgaacatca gctgcgttat caggaaacgg
      661 agtttgtgat tagttatgaa gacttccatc gtcctgaatt taccagcgtg ccattattta
      721 aagatgaaat ggtgctggta gccagcaaaa atcatccaac aattaagggc ccgttactga
      781 aacatgatgt ttataacgaa caacatgcgg cggtttcgct cgaacgtttc gcgtcattta
      841 gtcaaccttg gtatgacacg gtagataagc aagccagtat cgcgtatcag ggcatggcaa
      901 tgatgagcgt acttagcgtg gtgtcgcaaa cgcatttggt cgctattgcg ccgcgttggc
      961 tggctgaaga gttcgctgaa tccttagaat tacaggtatt accgctgccg ttaaaacaaa
     1021 acagcagaac ctgttatctc tcctggcatg aagctgccgg gcgcgataaa ggccatcagt
     1081 ggatggaaga acaattagtc tcaatttgca aacgctaact gaatgcagaa taggccagac
     1141 atgaatgtct ggtttattct gtatttttta ttgaatgtag aattttattc tgaatgtgtg
     1201 tgctctctat tttaggatta attaaaaaaa tagagaaatt gctgtaagtt gtgggattca
     1261 gccgatttat tgtcaattta atccttcgta atggaggatt ttatcgtttc ttttcacctt
     1321 tcctcctgtt tattcttatt accctgcgtt tatgtctctg gctgccaatt gcttaagcaa
     1381 gatcggacgg ttaatgtgtt ttacacattt tttccgtcaa acagtgaggc aggccatgga
     1441 gatgttgtct ggagccgaga tggtcgtccg atcgcttatc gatcagggcg ttaaacaagt
     1501 attcggttat cccggaggcg cagtccttga tatttacgat gcattgcata ccgtgggtgg
     1561 tattgatcat gtattagttc gtcatgagca ggcggcggtg catatggccg atggcctggc
     1621 gcgtgcgacc ggggaagtcg gcgtcgtgct ggtaacgtcg ggtccagggg cgaccaatgc
     1681 gattaccggc atcgccaccg cttatatgga ttccattcca ttagttgtcc tttccgggca
     1741 ggtagcgacc tcgttgatag gttacgatgc ctttcaggag tgcgacatgg tggggatttc
     1801 gcgaccggtg gttaaacaca gttttctggt taagcaaacg gaagacattc cgcaggtgct
     1861 gaaaaaggct ttctggctgg cggcaagtgg tcgtcctgga ccagtagtcg ttgatttacc
     1921 gaaagatatt cttaatccgg cgaacaaatt accctatgtc tggccggagt cggttagtat
     1981 gcgttcttac aatcccacta ctaccggaca taaagggcaa attaagcgtg ctctgcaaac
     2041 gctggtggcg gcaaaaaaac cggttgtcta cgtaggcggt ggggcaatca cggcgggctg
     2101 ccatcagcag ttgaaagaaa cggtggaggc gttgaatctg cccgttgttt cctcattgat
     2161 gggcctgggg gcatttccgg caacgcatcg tcaggcgctg ggcatgctgg gaatgcacgg
     2221 cacctacgaa gccaatatga cgatgcataa cgcggatgtg attttcgccg tcggggtaag
     2281 atttgatgac cgaacgacga acaatctggc aaagtactgc ccaaatgcca ctgttctgca
     2341 tatcgatatt gatcctactt ccatttctaa aaccgtgact gctgatatcc cgattgtggg
     2401 ggatgctcgc caggtcctcg aacaaatgct tgaactcctg tcgcaagaat ccgcccatca
     2461 accgctggat gagatccgcg actggtggca gcagattgaa cagtggcgcg ctcgtcagtg
     2521 cctgaaatat gacactcaca gtgaaaagat taaaccgcag gcggtgatcg agactctttg
     2581 gcggttgacg aagggagacg cttacgtgac gtccgatgtc gggcagcacc agatgtttgc
     2641 tgcactttat tatccattcg acaaaccgcg tcgctggatc aattccggtg gcctcggcac
     2701 gatgggtttt ggtttacctg cggcactggg cgtcaaaatg gcgtttccag aagaaaccgt
     2761 ggtttgcgtc actggcgacg gcagtattca gatgaacatc caggaactgt ctaccgcgtt
     2821 gcaatacgag ttgccggtac tggtggtgaa tctcaataac cgctatctgg ggatggtgaa
     2881 acagtggcag gacatgatct attccggccg tcattcacaa tcttatatgc aatcactacc
     2941 cgatttcgtc cgtctggcgg aagcctatgg tcacgtcggg atccagatct ctcatccgca
     3001 tgagctggaa agcaaactta gcgaggcgct ggaacaggtg cgcaataatc gcctggtgtt
     3061 tgttgatgtt accgtcgatg gcagtgagca cgtctacccg atgcagattc gcgggggcgg
     3121 aatggatgaa atgtggttaa gcaaaacgga gagaacctga ttatgcgccg gatattatca
     3181 gtcttactcg aaaatgaatc aggcgcgtta tcccgcgtga ttggcctttt ttcccagcgt
     3241 ggctacaaca ttgaaagcct gaccgttgcg ccaaccgacg atccgacatt atcgcgtatg
     3301 accatccaga ccgtgggcga tgaaaaagtg cttgagcaga tcgaaaagca attacacaag
     3361 ctggtcgatg tcttgcgcgt gagtgagttg gggcagggcg cgcatgttga gcgggaaatc
     3421 atgctggtga aaattcaggc cagcggttac gggcgtgacg aagtgaaacg taatacggaa
     3481 atattccgtg ggcaaattat cgatgtcacc ccctcgcttt ataccgttca attagcaggc
     3541 accagcgata agcttgatgc atttttagca tcgattcgcg atgtggcgaa aattgtggaa
     3601 gttgctcgct ctggtgtggt cggactttcg cgcggcgata aaataatgcg ttgagaatga
     3661 tctcagtgcg caatttgcag cccaacatgt cacgttgggc tttttttgcg aaatcagtgg
     3721 gaacctggaa taaaagcagt tgccgcagtt aattttctgc gcttagatgt taatgaattt
     3781 aacccatacc agtacaatgg ctatggtttt tacattttac gcaaggggca attgtgaaac
     3841 tggatgaaat cgctcggctg gcgggagtgt cgcggaccac tgcaagctat gttattaacg
     3901 gcaaagcgaa gcaataccgt gtgagcgaca aaaccgttga aaaagtcatg gctgtggtgc
     3961 gtgagcacaa ttaccacccg aacgccgtgg cagctgggct tcgtgctgga cgcacacgtt
     4021 ctattggtct tgtgatcccc gatctggaga acaccagcta tacccgcatc gctaactatc
     4081 ttgaacgcca ggcgcggcaa cggggttatc aactgctaat tgcctgctca gaagatcagc
     4141 cagacaacga aatgcgctgt attgagcacc ttttacagcg tcaggtcgat gccattattg
     4201 tttcgacgtc gttgcctcct gagcatcctt tttatcaacg ctgggctaac gacccgttcc
     4261 cgattgtcgc gctggaccgc gccctcgatc gtgaacactt caccagcgtg gttggtgccg
     4321 atcaggatga tgccgaaatg ctggcggaag agttacgtaa gtttcccgcc gagacggtgc
     4381 tttatcttgg cgcgctgccg gagctttctg tcagcttcct gcgtgaacaa ggtttccgta
     4441 ctgcctggaa agatgatccg cgcgaagtgc atttcctgta tgccaacagc tatgagcggg
     4501 aggcggctgc ccagttattc gaaaaatggc tggaaacgca tccgatgccg caggcgctgt
     4561 tcacaacgtc gtttgcgttg ttgcaagggg tgatggatgt cacgctacgt cgcgatggta
     4621 aactgccttc tgacctggca attgccacct ttggcgataa cgaactgctc gacttcttac
     4681 agtgcccggt gctggcagtg gctcaacgtc accgcgatgt cgcagagcgt gtgctggaga
     4741 ttgtcctggc aagcctggac gaaccgcgta agccaaaacc tggtttaacg cgcattaaac
     4801 gtaatctcta tcgccgcggt gtgctcagcc gtagctaagc cgcgaacaaa aatacgcgcc
     4861 aggtgaattt ccctctggcg cgtagagtac gggaatggac atcaa
//
LOCUS       DAZFRN010000260         4905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFRN010000260 DAZFRN010000000
VERSION     DAZFRN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.262"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(90..851)
                     /gene="kduD"
                     /locus_tag="ACHKUB_004919"
     CDS             complement(90..851)
                     /gene="kduD"
                     /locus_tag="ACHKUB_004919"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO6657811.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(881..1717)
                     /gene="kduI"
                     /locus_tag="ACHKUB_004920"
     CDS             complement(881..1717)
                     /gene="kduI"
                     /locus_tag="ACHKUB_004920"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO6657812.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSHFLERRELGVINIGGAGTITVDDQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2004..3185)
                     /gene="yqeF"
                     /locus_tag="ACHKUB_004921"
     CDS             complement(2004..3185)
                     /gene="yqeF"
                     /locus_tag="ACHKUB_004921"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO6657813.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3440..4669
                     /gene="yqeG"
                     /locus_tag="ACHKUB_004922"
     CDS             3440..4669
                     /gene="yqeG"
                     /locus_tag="ACHKUB_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6657814.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1288 a         1248 c         1064 g         1305 t
ORIGIN      
        1 ttatttcata accatgatat ggattatgat gatctacagg tataaaaaac cctgccatgc
       61 ggcagggtca taaaagtaag aagaatgaat taacgcgcca gccaaccgcc atccacggca
      121 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
      181 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
      241 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
      301 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac acccatcacg
      361 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
      421 gcgatattga taatcttgcc gccattgcct tgcgcgataa agtgtttcgc cgctgcctga
      481 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc cttttcgctg
      541 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
      601 ccaaactccg ctaccgcgcg atccagcagt ccaggaatgc catcaatctt tcgcagatcg
      661 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gctcgatggt ttcagtcggt
      721 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
      781 tggcccagcc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
      841 cttaaaatca taacaatacc ttattcgttt atgcccacaa ctagcgcaaa tctttaacgg
      901 ccacatggtc catatcatca aagacctggt tttcaccgac catgccccag ataaaggtat
      961 aagctttggt tccgacgccg gaatgaatcg accagctcgg ggagatcacc gcctgctcgt
     1021 tatgcatcac aatgtgacgc gtttcttgcg gctgccccat catgtggaaa acgcaggcgt
     1081 catcatccat attgaaatag aaataaactt ccatccggcg ctcgtgagtg tgacacggca
     1141 tggtgttcca caagttacct ggagccagct ccgtcagccc catactcaat tggcaggttt
     1201 ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg gttactggtg aggttatcgc
     1261 ctaacgtgac tggagatact tcgtccggtg tgactttttt ggtgggatac gtcgtatgcg
     1321 cgggtgcaca attgtaataa aactttgccg gagtggcggt atcaatactg gcaaagacaa
     1381 cttcttttgc acctttaccc acatacaggg cgtcgcggtg accgatttca tagcattggt
     1441 catcgacagt aatcgtaccg gcaccgccaa tattgataac acctaactcg cgacgttcaa
     1501 ggaaatggct tacgccgagt tgtttaccaa cttccccgcc aacggaaacc gttttagtta
     1561 tcggcataat gccgccaaca ataattcggt caatgtggct gtaaaccatg gtgtactcat
     1621 cggcgacaaa tacctcttca accaaaaatt cattgcgcag cccttgggta tccagcgttt
     1681 ttgcgtgcgc actgtggatg ctctgtctta cgtccacatc aacctccgat aatcagtgaa
     1741 gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt tctctataaa gggatgatat
     1801 gcccatgggc tagggttttc aattaaaaat aaaacaatgt ttcattttta agttaaggat
     1861 taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg agggagccca
     1921 gaaaatgtat gtcgacttgc cagccatcat tattcaacag attaatgacg gcagatattt
     1981 atcggtaagg aggaaatgca gagctactcg tcacgttcaa tggtcaatgc cacgccctgg
     2041 cccccgccga tacaaagcgt tgccagccct ttgcgggcat tacgtttcac catttcatga
     2101 accagagaaa ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg
     2161 ccaccattga cattgacccg acgctcatcc cactcaagca tcttgccaac cgaaagcgcc
     2221 tgtgcagcaa acgcttcatt agcctcgata agatcgacat cagccaactg ccagcctaca
     2281 cgctccaggc aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc cggatctaca
     2341 ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta aattcaacgc tcgtgctttg
     2401 gcttcgctca tcatcattac cgcagctgcg ccatcgttta tggatgatgc attccccgcg
     2461 gtcacagaac cgagactatc aaatgaagga tttaaacggg ctaaaccttc tgcactggcg
     2521 tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct gaccgttact ttggatcatt
     2581 accgggacga tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc
     2641 gagctaagtg cgtaagcatc ctgcaactga cggctgatgc catattcgcg agccagattt
     2701 tcggcggtga caccaatatg ataatcattg aaggcatccc acaacccatc atgcaccaga
     2761 ctgtcaacca actggctatt gccaagctgt gcaccggtgc ggctatcagt cagaacatgt
     2821 ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa tatcagcctc gccacactgt
     2881 atcgcctgag tagccagatg cagtgcttta agcccagaac cgcaaacgtc attaatagtg
     2941 attgcagaaa cgctattagg aagaccacct ttaatagccg attgccttgc cggattctgc
     3001 cctgcccctg cagtcaacac ctgaccaaga attacttcat ccaccgcata tgcaggaacg
     3061 ccggtacgtt ctattaacgc tttcacgacc agactaccaa gttccacggc ggaatgaccc
     3121 gctaacgcac cgcgaaagca gccgataggt gtccgtaacg ccccgacaat cacaacgtct
     3181 ttcatcacaa cctcgcacca attaacagtg caatagtaaa tgattgttaa ttacagttat
     3241 cttaattgtt taaaaaaagt gatttttatc acaaaggaaa tatgcctgag cagtagtcag
     3301 agtcataact ggcacgtaag gtttgcaacc actaacccac caatagaggg gtagataggg
     3361 cgttaatctc ccatacttaa cctggtttat ggtaaattgc cctccatttt gtttaatttg
     3421 tagatgatac gttcagataa tgtctaatat ttggtctaaa gaagaaactc tgtggagttt
     3481 cgcgctctac ggcacagccg ttggtgcagg cacgctcttc cttcctattc agttaggttc
     3541 ggcaggggct gtggtcctgt ttattactgc tctggtcgcc tggcctttaa catattggcc
     3601 acataaagcc ttatgccagt tcatcctctc atcgaaaaca tcagcaggtg aagggataac
     3661 gggcgcagta acacactact atggcaagaa gattggtaat ctgattacca cgctgtactt
     3721 catcgccttt tttgtcgtcg tgttgatata tgcagtggca attaccaact cacttacgga
     3781 acagctggca aagcatatgg ttattgatct tcgcatccgt atgttggtga gtctgggtgt
     3841 tgtattaatc ctgaatctca tttttctgat gggacgccat gcaaccattc gggtaatggg
     3901 ttttttggta ttcccattga ttgcctattt cttatttctt tccatttacc ttgtcggtag
     3961 ttggcaacct gatctattaa cgacccaggt agagttcaat cagaataccc ttcaccagat
     4021 atggatatcg attcccgtga tggttttcgc ctttagccat acgcccatta tttctacgtt
     4081 tgccatagac agacgtgaaa aatatggcga acacgctatg gataaatgca aaaaaattat
     4141 gaaagtcgct tatctcatca tctgcataag tgtactgttc tttgtcttta gctgcctgct
     4201 ttctattcca ccttcgtata ttgaagcggc taaagaagaa ggggtcacca ttttatcggc
     4261 gctttctatg ctgccgaacg ccccagcatg gctgtcaatt tccgggatta ttgtcgcagt
     4321 agttgcgatg tcgaaatcat tcctgggtac gtactttggc gttattgaag gtgccacgga
     4381 ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa agtcgtgcat ttaaccgcgc
     4441 actatcaatt atgttggtat cgctgatcac cttcattgtt tgttgcatta acccgaacgc
     4501 gatttcgatg atttacgcga tcagcggccc gctcattgcc atgatacttt tcatcatgcc
     4561 tacgttgtca acgtatctga tcccggcgct taaaccctgg cgttccatcg gaaatctgat
     4621 tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc tttagctgat atccattgtg
     4681 aatggttcaa aattgctaca attttgaacc attccagttt tcataatgtt cattcgttat
     4741 tattccgcac tatcacttcc ctcatcatat cctagtatta tcagttagtt aaacacacaa
     4801 agagatctga acatcttaaa taagactatt taagatgcat aacttagatt cgcaagatat
     4861 cttccggtca ttcaaaaaca atttgcgata tagatattct catac
//
LOCUS       DAZFRN010000261         4874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFRN010000261 DAZFRN010000000
VERSION     DAZFRN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.263"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHKUB_004923"
     CDS             complement(<1..617)
                     /locus_tag="ACHKUB_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657815.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINASVYD"
     gene            complement(623..901)
                     /locus_tag="ACHKUB_004924"
     CDS             complement(623..901)
                     /locus_tag="ACHKUB_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657816.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(916..2184)
                     /locus_tag="ACHKUB_004925"
     CDS             complement(916..2184)
                     /locus_tag="ACHKUB_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6657817.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(2181..3806)
                     /locus_tag="ACHKUB_004926"
     CDS             complement(2181..3806)
                     /locus_tag="ACHKUB_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657818.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(4344..4862)
                     /locus_tag="ACHKUB_004927"
     CDS             complement(4344..4862)
                     /locus_tag="ACHKUB_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO6657819.1"
                     /translation="MTGISRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKIT
                     DLLTAEKARVMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGK
                     LPEAFFWTDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKDLESLSSAD
                     EILAYRVGWAQE"
BASE COUNT         1209 a         1387 c         1012 g         1266 t
ORIGIN      
        1 taatcatata ctgatgcatt aataaccagg accttatcta tatacggctt tcgaatatcc
       61 catgttgcac caccggaata atgctcacag gaatatattt tatttcctgt agcaccagtg
      121 gatgtggtat aaatcggtcg atcataagga gttcttttcc agttataata cccgaccagt
      181 gcaggcatga ttgcgcatgg atatccaagg tccttttcaa atttgatatc aacaggtatt
      241 aacttcgcgt caagcagcat catttcgcca tgataaatca tctcaccgtc cgggttatac
      301 atggcgatac catactcaga aggtggagta accatattcg cgaaagcata aaccgtcgta
      361 acaccagggt tcgttcccct gacaattaca tgaagcctta gtgcatgata tccatcaatc
      421 tgttcatgcg tgtacatgac atcggccttc ttctctgttc tgataaagaa aaaacaactt
      481 ttgcctgacg ggattgatgt tttaaaaaaa gacttttcag tcgccggtat ggtgcctttg
      541 ttgatcagac actgcggcgt aaaacctgga cttatccata cgctgccatc cggcttcata
      601 atgctcaaac catacatgac acttatcccc agaatgtata aatataagat cccataccct
      661 gctcaagatt cgaccacgtc accgtattgc cattaatggt tatcttcggt actttccgat
      721 ccgcaaatac gttattccag ggaaataaac aacatacagc ctgcaatgat ttcccgtcgg
      781 gtttattcgt gtacgtcttt gagccagact ccgctgtaaa tctgtccagg aaaaataccg
      841 gagtcagcac gcccgtaaca ttaacgttat ttctgttata aatggcaaaa ccgtattcca
      901 atactcacct cctgatcagc gtaatctgcc tatgcgaaca gccagtcgtc cattctgatc
      961 ataaacctca attttatcat tgcggatcac cagtcctaca ttctgattag agtaacgaat
     1021 tgtcagttgc ccttgtgacg taacactgaa aaggcctcca atattcaggt taccctgaga
     1081 atcaacctga aagtttccgt tctgaataac ggcactccgg ataactggcg aagtgatact
     1141 taccccggct tttacctcat ccgccacaac cttccgcgac accagtgttt caatcaccgc
     1201 gtcataaatc atcgctttcg ggatcacaac cttgccacct gataccgcaa acggataggc
     1261 ggtgttatcc gggttgttcg ggtcaaagac aaacaactgc gacgcagaaa ttgcaacctg
     1321 acttacaggc ctgccttcac tgtcttttcc ggcgacaatc ccgatccccg cagtgatacc
     1381 atcaactccc gctttttttg accacattgc cagaaacgcc tcaccgcctt ctttatccag
     1441 tttagtgatg cgcttgtcga cctcattaag cgattcactg gttgatgaat ccagtgtgct
     1501 aatgcgggtt tcaataccac caatcgttct tgtcgtttct tccctgagag tccccacaac
     1561 ttcggttgtc ttaattgcag catcctttac agcctgcccc tgcgcgtttt ttatttcttt
     1621 acgcagctcg gacacaaccg gcgactttgc agcctcatcg cggatctggt caatgatggc
     1681 cttcacgccg atctgtgttt gtgcctgagt gcctttttca gcattccatg gacctttcac
     1741 tcctgccgcg ttaacaaaac gtatccagta aaatcccgac cagccagggt caaccggatc
     1801 gccgtaaacc tgccccggcg tcgtggcaac cagcactgca tcagcaaggt catcctccgt
     1861 accccgccag atttcagtca gtgaatgtcc gcgataatta ggcatatccc attcaagaag
     1921 aaccgagcca aatcctcctg tcgccttaaa attcagcggt tttgtgggaa aatcaacagt
     1981 cattaaagta ctgtcaatct caatacccgg attcagtgca tatgaggcac cacccgatgt
     2041 tcgacgccgg gcgagtttaa gaccaaccag ttcctcacgg gtcacaaatg cgtggcgtcc
     2101 gtcaccacgc tgcccggtgc caatttccat gttctccaca actgtggata aatccttccc
     2161 cgcacgccac ggttttctgg tcataccggc atctccgaca tcgatgtact cagggttatt
     2221 cgttccacct gcccgaatcc ggataccatc acctgccagt tttgcccggt tgctgccgga
     2281 agtctcacca cacttccctt aaacgtaccc ggcgcaaaat gaatcacagg aacatcatca
     2341 gccataatgg tgatccccac ccgctcaggc gccggagatt tcaccctgat acaggaaaaa
     2401 gaggttcttt caggtaatga aaaaattttt gaatgccacc ttatcgtgga gggcagagag
     2461 cccccggcaa gcactgacat tttgtctcct gtcaccacgc gcatcatatc tttcgcgaga
     2521 tcaacccatg cgcagtcaaa cggtgtactg agataacgga tatccatgtt caccggactg
     2581 aatacaaaca catcctgctt accatccggt ttcgtgtaac aggcaatgta ctcaccacgc
     2641 caggaataag ccacaatgga cgccgggtta aactgactct gccactgttc aggtgaaata
     2701 atcttttccg ttgccagtgc tgtattaccg tttacatcaa cagataccag accgtttgtc
     2761 ccagcataga gtacgaatcc ctccatcgcc accatacttc ttcggctcag gcatgcctgc
     2821 atggaaggaa ttctggagcc agaaattgtg gaaggcgata caccactgaa cagataaggc
     2881 tccccctttg tcgccaccac cagtgacgtt cccagcggac agatagccac aatatcttct
     2941 gccgtcgtgt gacgattcac ttccggccat gcatacggca gatacgcttc cgaaaacatc
     3001 acttcattac cggcaaaccc ggcggcaata ccattagcca tcaggcaaag gcctgtcata
     3061 ttctctggcg gcggcaggta atcccatgtc gccagagaag gtccaaggtt tttccccggt
     3121 attttgtccg tgtaactgag cacggatgca tccagttcag ccacaagtaa aaaatccgct
     3181 tctcctccac ctgatgcaga gcgataaatc cggcggcgtt taatactggc attctgcaat
     3241 ggcaccggag acagcgtcag ctgtaccgca gtccccggag tacggagtgt tacctccaga
     3301 gacgccggac ctggcggacc ttcttcacca taatctgaga caaaggtttc cgtataaaac
     3361 cgggtttcgt catcattcgg gttatcgtca gaaacatcac cgccctgctg aacagtacag
     3421 acaggtgctg tcgtcggcgc ggggatcccc agacgatacg atgatgtcgg gtgattcccg
     3481 tccccttttg tggcaatggt cgcatccgtc actttaggaa aacgcccgtc agtgtagtaa
     3541 atacgcccgt gggagtcctg agcgaccgga ctgcggatca catccaccac atccgtccat
     3601 gcaaaccaga aatcgtcacg gtaatgaaaa atggttttcg gcttaatcgc gaatgttttc
     3661 tcagcctctg acatctggtg ttcaggcgtg atcactccat agcgaaaatg acagttttct
     3721 gccagtacag cagaatgatc tggcagcata gatgcaataa cgcctggcat catcccgcgc
     3781 atagttgtta tatcgatata aggcatggtt gtctggtatc cgaaaatttg aatggcaggt
     3841 gagtcactga attgaagttt taaaggcggc atacaattgc gcctgtgcag aatacagatt
     3901 tccagaaatc tgaaaaatta ctcttatcag agaagagcgt cagaaacacg atcggcatca
     3961 ctgtttctgc aaacttccct gtggcagtct ttattcgcca tataacgtag cactaaaggt
     4021 cttcattaat ttttccattg tagttactcc ttccttgtac cgggggttgg tcatgccccc
     4081 gttttttatt ccctctgctc ctttaatcgc actgccaagc gccacaacac acatcaactg
     4141 tggctggtat ggaaacacca aaaactctca cttccggaaa tctgaaaaat tatccatatc
     4201 aacaggatgc gccggaaaca tgatttccat cacatccagg cctgcacttt ccgtgacagt
     4261 atttattcgc atagtaaata cttcacaagt gtataattga ctcagttcat gggggaggtg
     4321 atggtccccc atctttttgt ctgttattcc tgtgcccagc caaccctgta tgccaggatc
     4381 tcatccgcac tgctcagcga ttccaggtcc ttcttcatgg tgcgctgccg gacgtggatt
     4441 tccatccctt tagtgaacat cgcctgctct gccgcttcac tcagcgcaat aagctcttct
     4501 gctgtcaccg gcacatcatt gttttccgca tccgtccaga aaaacgcctc cggcagtttc
     4561 cccgctttcg ccgccgccac cgacggctca agacgcgtct gcgttgactt cccgtagtcc
     4621 catttacgcc cattgtgctc aaacgtgtag ttcgccgctt ccatcgcatt acgccaggcg
     4681 ttgatttcat ctcccttcat cacgcgcgct ttctccgcgg tcagcaggtc cgtgattttc
     4741 tccccgtcaa aaccccagcg ccccgagatg tcagctttgc ggttttcttc cgtatccgcc
     4801 acctccacca cactctggcc cactggccac agaactgatg ccaccctgct gatgccggta
     4861 atgaccctgg cact
//
LOCUS       DAZFRN010000262         4870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFRN010000262 DAZFRN010000000
VERSION     DAZFRN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.264"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            67..666
                     /locus_tag="ACHKUB_004928"
     CDS             67..666
                     /locus_tag="ACHKUB_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6657820.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVSG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            731..4081
                     /locus_tag="ACHKUB_004929"
     CDS             731..4081
                     /locus_tag="ACHKUB_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6657821.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSASEAATRATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKASEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSA
                     ATSASAATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRRVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEGWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAVAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGAHTHSMTFVSG
                     GSSGAPGSGSPDYSKYSVNTSSAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSH
                     GHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            4081..4662
                     /locus_tag="ACHKUB_004930"
     CDS             4081..4662
                     /locus_tag="ACHKUB_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6657822.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENATWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQAANEHIAPLQDAVDLEIATEEE
                     NSLLEAWKKYRVLLNRVNTTTAPDIEWPVAPIG"
BASE COUNT         1273 a         1173 c         1349 g         1075 t
ORIGIN      
        1 attttataac ccataacggg cgtcagaaat gacgcctttt ttattgcaga aaagcgagag
       61 gtaattatgc gtaaacttta tgccgccatt ttgtccgcag ccatctgtct ggccgtatcc
      121 ggtgcgcctg catgggcgtc tgaacatcag tccacgctga gcgcgggcta tcttcatgcc
      181 tcgacgaacg tttccggcag cgatgatctg aacgggatta acgtgaaata ccgttatgag
      241 tttacggaca ctctgggaat ggtgacgtca ttcagctatg caggagacaa gaatcgccag
      301 ctgacccgtt acagcgatac ccgctggcat gaagattccg ttcgtaaccg ctggttcagc
      361 gtaatggcgg ggccgtctgt gcgcgtgaat gaatggttca gcgcgtatgc gatggcgggt
      421 gtagcttaca gccgtgtgtc gactttctcc ggggattatc ttcgcgtaac tgacaacaag
      481 gggaaaacgc acgacgtgct gaccggaagt gatgacggtc gccacagcaa cacgtctctg
      541 gcgtggggag ctggcgtgca gtttaacccg accgaatccg tggccattga tattgcttat
      601 gaaggctccg gcagtggtga ctggcgcact gacggtttca tcgtgggtgt cggttataaa
      661 ttctgattag ccaggtaaca cagtgttatg acagcccgcc ggttcaggcg ggcttttttg
      721 tggggtgaat atggcagtaa agatttcagg tgtactgaaa gacggcacag gaaaaccggt
      781 acagaactgc acaatccagc tgaaagcaaa acgtaacagt accactgtgg tggtgaacac
      841 gctggcctca gaaaatccgg atgaagccgg gcgttacagc atggacgttg agtacggtca
      901 gtacagcgtt attctgttgg tggaaggatt cccgccgtca catgccggga ccatcaccgt
      961 gtatgaagat tctcaacccg gtacgctgaa tgattttctc ggtgccatga cggaggatga
     1021 tgcccgtccg gaggcactgc gtcgttttga actgatggtg gaagaggtgg cgcgtaacgc
     1081 gtccgtggtg gcacagaaca cggcagccgc aaagaagtca gccagtgatg ccagcacatc
     1141 tgccagtgag gcggcaaccc gtgcgactga tgctgcaggc tcagcacgcg cagccagcac
     1201 gtcagccgga caggccgcgt cgtcggctca gtcagcatct tccagcgcag gaacggcatc
     1261 aacaaaggcc agtgaagcct caaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc
     1321 agctaccagt gccgctgcgg cgaaaacgtc agaaacgaat gcggcagcgt cacaacaatc
     1381 agcagccact tctgcatccg ccgcgaccac gaaggcgtca gaagcagcca cctcagcccg
     1441 ggatgcggcg gcctcaaaag aggcagcgaa atcatcagaa acgaacgcat catcaagcgc
     1501 cagtagtgcc gcttcctcgg caacggcggc aggcaattcc gcgaaggcgg caaagacgtc
     1561 tgagacaaac gccaggtctt ctgaaacggc agcgggacag agcgcctcgg ctgcggcagg
     1621 ctcaaaaaca gcggctgcgt cgtctgccag tgccgcgtca acaagtgccg ggcaggcctc
     1681 agccagtgcc accgccgccg gaaaatcggc agaaagtgcc gcatcgtctg cttcaacagc
     1741 cacaacgaag gctggcgaag ccactgaaca ggccagcgca gcagcgaggt ctgcttccgc
     1801 agcgaagaca tccgagacga acgcgaaagc gtcggaaacc agcgcagaat cctcaaaaac
     1861 ggctgccgca tcgtccgcca gttcggcggc gtcatcggca tcatcggcgt ctgcttcaaa
     1921 agatgaggcg accagacaag cgtccgcagc gaagggcagc gccacgacag catccacgaa
     1981 ggcgacagag gcagctggca gtgcgacggc ggcagctcag agcaaaagta cggcggaatc
     2041 cgcggcaacg cgcgctgaga cagcggcaaa acgggcagag gatattgcat ccgccgtggc
     2101 gcttgaggat gcgagcacga cgaaaaaggg gatagtacag ctcagcagtg cgaccaacag
     2161 tacgtctgaa acgctggcgg caacgccaaa ggcagtaaaa tcagcctatg acaatgcaga
     2221 gaaacgtctg cagaaagacc agaacggcgc tgatataccc gataagggac gcttcctgaa
     2281 caacattaac gcggtcagta aaacagactt tgctgataag cgtggtatgc gttatgtgcg
     2341 ggttaacgct cctgcaggtg caacatctgg aaaatattac cctgttgttg ttatgcgttc
     2401 tgctggctca gtaagcgaac tggcatcaag ggtcattatc accacggcaa cgcgaaccgc
     2461 aggcgatccg atgaataact gcgagtttaa tggatttgtt atgcctggtg gctggactga
     2521 cagggggcgt tatgcttatg gcatgttctg gcaatatcaa aacaatgaac gagccatcca
     2581 ctcaataatg atgagtaata agggcgatga tttgcgctct gtgttctatg ttgatggcgc
     2641 tgctttccct gtttttgcgt ttatcgaaga tggcctgtca atatccgcac ctggtgctga
     2701 tctcgttgtt aatgatacga cctataagtt tggggcaaca aatccggcga ctgaatgtat
     2761 cgcggcggac gttatccttg attttaagag tgggcgtggt ttttatgagt ctcattcgtt
     2821 aatcgttaac gataacttgt cgtgcaaaaa actttttgcc acagacgaaa ttgtagcgcg
     2881 tggtggtaat cagattcgaa tgataggtgg ggagtatggg gcattatggc gtaatgatgg
     2941 cgctaaaact tacctgctgc ttaccaatca aggtgatgtt tatggtggct ggaatacatt
     3001 aagaccgttt gctattgata acgcaactgg cgaactggtt attggaacca aactgtccgc
     3061 aagtctgaac ggtaatgcat taacagcaac aaagctgcaa acgccaagac gggtttctgg
     3121 tgttgagttt gatggttcca aagatattac tttaaccgcc gcgcatgtgg ctgcttttgc
     3181 cagaagggca acggatacat atgccgatgc ggatggtggc gttccctgga atgccgaatc
     3241 aggcgcttat aatgtcaccc gctctggcga cagctatatt ctggttaact tctataccgg
     3301 agtcggaagt tgccggaccc tgcagatgaa ggcgcattac agaaatggag gtctgttcta
     3361 ccgttcctca agagatggct atggttttga ggaaggctgg gcagaagttt atacctcgaa
     3421 aaatcttcca ccagaaagct acccagttgg cgcaccaatc ccgtggccat cagataccgt
     3481 tccgtctggt tatgccctga tgcaggggca gacttttgac aaatctgctt acccgaaact
     3541 tgcagttgct tatccgtcag gcgttattcc tgatatgcgt ggctggacga ttaagggcaa
     3601 gcccgccagt ggtcgtgccg tattgtctca ggaacaggac ggcattaaat cgcacaccca
     3661 cagcgccagc gcatccagta cggatttggg gacgaaaacc acatcgtcgt ttgattacgg
     3721 cactaaatcc acgaataaca ctggtgcgca tacccatagt gttagcggta cggctgcttc
     3781 agccggtgca catacccatt cgatgacatt tgtttcaggt ggttccagtg gtgctccggg
     3841 aagtggatca cctgattatt ctaaatacag tgttaacact tcttctgcag gcgctcatac
     3901 gcactctgta tcgggtactg ctgcaagcgc aggtgcacac gcacatactg tcggtattgg
     3961 tgctcatacg cactccgttg cgattggttc acatggacat accatcaccg ttaacgctgc
     4021 tggtaacgcg gaaaacaccg tcaaaaacat cgcatttaac tatattgtga ggcttgcata
     4081 atggcattca gaatgagtga acaaccacgg accataaaaa tttataatct gctggccgga
     4141 actaatgaat ttattggtga aggtgacgca tatattccgc ctcatacagg tctgccagca
     4201 aacagtaccg atattgcacc gccagatatt ccggcaggct tcgtggctgt tttcaacagt
     4261 gatgaggcat cgtggcatct cgttgaagat catcggggta aaacggttta tgacgtggct
     4321 tccggcgacg cgttatttat ttctgaactc ggtccgttac cggaaaatgc cacttggtta
     4381 tccccggaag gggagtttca gaagtggaac ggcacagcct gggtgaaaga tgcagaagca
     4441 gaaaaactat tccggatccg ggaggcggaa gaaacaaaaa acagcctgat gcaggcagcg
     4501 aatgagcata ttgcgccact tcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
     4561 aactcgttgc tggaagcctg gaaaaagtat cgggtgttgc tgaaccgtgt taatacaaca
     4621 actgcaccgg atattgaatg gccagtagca cctatagggt aaattcgtaa tgattaccta
     4681 aatatgttat tcttttgttc aaaaagtgat tttgctatag gtagggagta tgttcttgga
     4741 ttaagaataa aaaatcgcca tgaaagagct tcatggcgag tacataaacg tacaaaatgt
     4801 gatgaaagta gagtggcgta agccactata ttaagatatg acaatagcga acaaaattaa
     4861 ataacatttt
//
LOCUS       DAZFRN010000263         4808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFRN010000263 DAZFRN010000000
VERSION     DAZFRN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.265"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            483..1049
                     /locus_tag="ACHKUB_004931"
     CDS             483..1049
                     /locus_tag="ACHKUB_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6657823.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMLARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHVVFWIDRQRVKKIY
                     PFAERIAECWQAITYNCGSAHRCTYQPHYAYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            1295..1867
                     /locus_tag="ACHKUB_004932"
     CDS             1295..1867
                     /locus_tag="ACHKUB_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657824.1"
                     /translation="MNAIPYFDYSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPWEYDNAFSSRKIWLGMDFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKQYQKQITRNVVLDSRSPDCEERLKELIWAF
                     SDFARDRRGVHDPEARYLAGNPWYPVAGQL"
     gene            1936..2172
                     /locus_tag="ACHKUB_004933"
     CDS             1936..2172
                     /locus_tag="ACHKUB_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6657825.1"
                     /translation="MLTSITGHDSVLLRVDDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2436..2948
                     /locus_tag="ACHKUB_004934"
     CDS             2436..2948
                     /locus_tag="ACHKUB_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657826.1"
                     /translation="MQISEALISEPGKIQRFVQQAVDHWPHLLAVHFTLDSAEGNIDE
                     GQIHAFCTAFYRQVQERITERNHMASPASPLVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASVTVDEQCSQVVDLLQHSWQVISAGGQCRVERCFRVARGDTSGQYVALKTVALSL
                     GLPVVTTISW"
     gene            3037..3660
                     /locus_tag="ACHKUB_004935"
     CDS             3037..3660
                     /locus_tag="ACHKUB_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6657827.1"
                     /translation="MRTRIDYLADKYSFTERNESPRLRRQWQDVLEECRLTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGLSQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPFQQIAREVKAPRERLKYALETRLQVTALD
                     GLFWFGSQRIAADILRLRKSGMPVVTTSVEASDNLTGTTRKIPAYHL"
     gene            3757..3990
                     /locus_tag="ACHKUB_004936"
     CDS             3757..3990
                     /locus_tag="ACHKUB_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657828.1"
                     /translation="MARSDYDFINLSLGHELNEWLAERGYAGQADNRNRLAEVVTRKL
                     RDSFYVDVSWDALNVAYSEHPEWFSELASGDED"
     gene            4354..>4808
                     /locus_tag="ACHKUB_004937"
     CDS             4354..>4808
                     /locus_tag="ACHKUB_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFO6657829.1"
                     /translation="MQHRQDNLLANRNFLPGMVSGQCAFRLRTFSPVARHFSFLPCLC
                     ILSFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPEMSAGTEGP
                     CFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTTVTDAVTDGYIR"
BASE COUNT         1132 a         1153 c         1225 g         1298 t
ORIGIN      
        1 aatggtatgt ttcattctgg taccgtgtac ccgttaacgt tcatatcttg aacatgagtt
       61 gtttttaacc gattaaatga gtgcgtgtaa gtttattggg ttacaggtat gtgttggcct
      121 tgtacacggt ttttacaacc actgagagca agtttgtagt ttatgatgtg attggtcgca
      181 atatgtttct taacctccag gtagtggggt tttagctcgt attttacagt atttcgtgat
      241 gtttattgat actgtatttt attcgctcct cgtttatctc atctctttag ctaataccat
      301 cagataatcc atttctttct gcataatgct gcgtatcgtt aataacccgt cgtatccatt
      361 ctgctacagc atgcctgata aataccatct gtaagttatt accgttttag atctgattat
      421 gagcgaaagc attaattcgt tcacagagct taaaacatca ttaactttca ggagtcatca
      481 acatgcctaa atcttacaca ccaaactggt tttttaccgc tttacttgat aatcacatca
      541 atcaaatgct ggcacgctat tcctgcctgc gggccttacg gatggatttc ttctacagga
      601 aagatacgcc cgatttctta caacctgatc atcgctggct tgaattgcag ttgcgtatgc
      661 tgctggagca ggtggaacaa tttgaaaata tcgttggttt cttctgggtg attgaatgga
      721 cggctgatca tggttttcat gcgcatgtcg ttttctggat cgatcgtcag agggttaaaa
      781 aaatatatcc ctttgcggag cggatagcgg aatgctggca ggcaattacg tataactgtg
      841 gttcggcgca ccgctgtaca tatcagccgc attatgcata caacatcaac attcctgtgc
      901 gccacaacga tcctgaaagc atcgataata ttcgcggtgc cctgcattat ctggcgaaag
      961 aagagcaaaa agacgggctg tgtgcttacg gctgcaatga agttcctgaa cgtcctgctg
     1021 cagggcgtcc tcgtaagcct cacttctgaa gcttaaggcc tgagccttcg ctcctggaaa
     1081 cactccgtcg gtaaaaactt accgccttga ttaatgatgt gaaccgaagt caacggagat
     1141 cattcaccct gaaccacatc cggtgttttg ttccttgtct tcccgttctg ctccggttct
     1201 tcacttattc catcaatctc atccgcaagc cataacacgt cagctcattc acgggcagga
     1261 cgcattgtgg gctgcgcata acggaacata tcttatgaat gctattcctt atttcgacta
     1321 tagcctggcc cccttctggc cgtcttatca gagcaaagtt atcggcgtcc ttgagcgtgc
     1381 gctgcgtgag cagtccggct cacggatacg gcggatcctg cttcgtctgc cgtgggaata
     1441 tgacaacgcc ttcagcagca gaaagatctg gttaggcatg gattttatcg aaaccgtcag
     1501 tgcgctgatg aatgcaaccc ccggacgtga tctctgctgg ctcctgaccc gtcatcctga
     1561 aaaacctgaa taccacgtgg tgctgtgcgt cagacaggag tatttcgatg gccctgaact
     1621 ggaccggttg atactggatg cctggagtaa cgtactgggc ttcgcgtcac cgggtgaagc
     1681 aaagcagtat cagaagcaga tcacccggaa tgtggtgctg gacagtcgtt caccggactg
     1741 cgaagagaga ctcaaagagt tgatatgggc gttcagtgat ttcgcccgcg atcgtcgtgg
     1801 tgtgcacgat ccggaagccc gttacctcgc cggtaatccc tggtatcccg tagcaggcca
     1861 gctctgaaag ctcagttcag cagattcccc cgcattcgcc taaccttcat aaatctcaca
     1921 gaggatattc tgaccatgtt gacctcaata acaggccacg acagcgtatt gctgcgtgtc
     1981 gacgatcccc tgatcgacat gaactacatc accagtttca ccggtatgac agataaatgg
     2041 ttttacaagc tgatcagtga aggacatttt cctaaaccca tcaagctggg ccgcagcagt
     2101 cgctggtaca aaagcgaagt ggagcagtgg atgcaacagc gaatcgagga gtcacgagga
     2161 gcagcagcat gaaacgtgtt gtaatgccag tacgctggca atgtgcgaaa tgccggcgct
     2221 ggtattgtgg aacccggccc tgcccctggt gctggcgata ttcccgttca tcttcctgct
     2281 ggtaccttcc ggtcagcagc gggttgttat cccttcctga ctgattcgtc attctattct
     2341 tattgattat aactggcatt acaccggtgc cggcgtgctt ttctgtagat ctgcaccggt
     2401 ttaacaaaat tagacaaggt ttgaaggaat attctgtgca aataagcgag gccctaattt
     2461 cagaaccggg gaaaatccag cgttttgttc agcaggcggt ggaccactgg ccacatctgc
     2521 tggcagtcca cttcacgctt gattcggctg aagggaatat tgacgagggg cagattcatg
     2581 cattctgtac tgccttttac cgacaggtgc aagaacgtat tactgagcgt aatcacatgg
     2641 ccagtccggc atcgccgctg gtattacgct ggttgcggga acaacatgga ggagcaacaa
     2701 ttcgatgcct gttgctgctc agccagacga gtatttgtca cccgcgagcc agtgtcacag
     2761 ttgatgaaca atgttcgcaa gtggtggatt tactgcaaca tagctggcag gtgataagtg
     2821 ctggcggaca atgccgggtg gaaaggtgtt ttcgggttgc ccggggtgat acatccggtc
     2881 agtatgttgc gttaaaaaca gtcgcattgt ctctggggtt accggttgtg acaacaatca
     2941 gttggtaatg gacgtgtgaa ccatctgcgc ttccgttgat ttttatggac tgataaagtt
     3001 ttgccagctg aatctttata cggaatgctc ttcagtatgc gtacacgaat tgactatctg
     3061 gctgataaat actcttttac cgaacggaat gaatctccac gccttcgccg gcagtggcag
     3121 gatgttctgg aggagtgtcg gctgacagag gccggaccag aagaacggct gcgtattgcc
     3181 ctgctgaatg tggattacgt caccagtttt gaactgcctt ttcggctgct tttaacccgc
     3241 acgccgcaac tgattgccgc gcttcgggaa gaatggggcc tcagccagaa aaatgtggtg
     3301 ttcaacgata aacggtttgg ctgcgtgtac agcctgaagg ccagcctttc cggtgtgccg
     3361 gatacatacc ggtatcatct gtctcatcgt attcgccggg tggttgggaa tgaaaacaca
     3421 tcattgccat ttcagcagat tgcccgggaa gtgaaagcgc cccgtgaacg actgaagtat
     3481 gcgctggaga cgagattgca ggtgaccgca ctggacggcc tgttctggtt cggcagccag
     3541 cgcattgcgg ctgatatcct gagactgaga aagagcggaa tgccggtggt gacaacgtcc
     3601 gtggaagcga gcgataacct gacgggaaca acccgcaaaa taccggcata ccatctctga
     3661 cattgcgatg aagggcagat ttcaccttga caggggcaga gtgccgcttt ttatacttta
     3721 ttcccgtgtc tgaaaaaatg tgcaaaggaa acgggaatgg caaggtccga ttacgatttt
     3781 atcaatctgt ctctgggaca tgaactgaat gagtggctgg cagagagagg ttatgccgga
     3841 caggcggata accggaaccg actggcagag gtggttaccc gcaaattgcg ggacagtttt
     3901 tatgtggacg tctcctggga tgcgctgaat gtggcataca gtgaacaccc tgagtggttt
     3961 tcagagcttg cctccgggga tgaggattaa caggcaaatt atgctgctat cgggcagagt
     4021 gattacctgc agggatttcc atttataaga atacgccgct tcgggaaagc tccggttctc
     4081 cggagagtta cgattatttt tactcaaatt cacaacacct gaactggaac ttgcgttgtg
     4141 tcccggattg ttactccgca ggaagcatcc tttttaccat acggatgttt gttttccatt
     4201 tcccctccga aaaatacaac tccgatcaca tttctgatat tttccccgga ttttacataa
     4261 caggattgtt tctgtatgtt ttttatctgg tgtaaatttc agcactgaca ttccacttac
     4321 gttaatttac actggatacc ccacgaggag aatatgcagc accggcagga taacttactg
     4381 gcgaacagaa attttttgcc tggtatggtt tccggtcagt gcgcattcag gctccgcact
     4441 ttctctccgg tggcacgcca tttttccttc ctcccctgcc tttgtattct ttcgttttct
     4501 tctccggcag ccatgctgtc tccgggtgac cgcagtgcaa ttcagcagca acagcagcag
     4561 ttgctggatg aaaatcagcg tcagcgtgat gcgctggagc gcagtgcgcc gctgaccatc
     4621 acgccgtctc cggaaatgtc tgccggtact gaaggtccct gctttacggt gtcacgcatt
     4681 gttgtcagtg gggccacccg actgacgtct gcagaaaccg acagactggt ggcaccgtgg
     4741 gtgaatcagt gtctgaatat cacggggctg accacggtca cggatgctgt gacggacggc
     4801 tatatacg
//
LOCUS       DAZFRN010000264         4798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFRN010000264 DAZFRN010000000
VERSION     DAZFRN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.266"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            157..1131
                     /gene="hemB"
                     /locus_tag="ACHKUB_004938"
     CDS             157..1131
                     /gene="hemB"
                     /locus_tag="ACHKUB_004938"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO6657830.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1237..2088)
                     /gene="tauD"
                     /locus_tag="ACHKUB_004939"
     CDS             complement(1237..2088)
                     /gene="tauD"
                     /locus_tag="ACHKUB_004939"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO6657831.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2085..2912)
                     /gene="tauC"
                     /locus_tag="ACHKUB_004940"
     CDS             complement(2085..2912)
                     /gene="tauC"
                     /locus_tag="ACHKUB_004940"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO6657832.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2909..3676)
                     /gene="tauB"
                     /locus_tag="ACHKUB_004941"
     CDS             complement(2909..3676)
                     /gene="tauB"
                     /locus_tag="ACHKUB_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO6657833.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3689..4651)
                     /gene="tauA"
                     /locus_tag="ACHKUB_004942"
     CDS             complement(3689..4651)
                     /gene="tauA"
                     /locus_tag="ACHKUB_004942"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6657834.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1092 a         1391 c         1241 g         1074 t
ORIGIN      
        1 tttcagtgaa tatgaaatta ttgctatttc tattacaaag atgtcctggg ctctctttca
       61 ttctcttctt gtcaacatcg cgacaacttt cgtaaaacac tcctgccccg cttcaggtat
      121 actgtgcacc ttaattccac aaacatcagg cagaccatga cagacttaat ccaacgccct
      181 cgtcgcctgc gcaaatctcc tgcgctgcgc gctatgtttg aagagacaac acttagcctt
      241 aacgacctgg tgttgccgat ctttgttgaa gaagaaattg acgactacaa agccgttgaa
      301 gccatgccag gcgtgatgcg cattccagag aaacatctgg cacgcgaaat tgaacgcatc
      361 gccaacgccg gtattcgttc cgtgatgact tttggcatct ctcaccatac cgatgaaacc
      421 ggcagcgatg cctggcggga agatggactg gtggcgcgta tgtcgcgcat ctgcaagcag
      481 accgtgccag aaatgatcgt catgtcagac acctgcttct gtgaatacac ttctcacggt
      541 cactgcggtg tgctgtgcga gcatggcgtc gacaacgacg cgactctgga aaatttaggc
      601 aagcaagccg tggttgcagc tgctgcaggc gcagacttca tcgcaccttc cgccgcgatg
      661 gatggccagg tacaggctat tcgccaggcg ctggacgctg caggctttaa agatacggcg
      721 attatgtcgt attcgaccaa gttcgcctct tccttttatg gtccgttccg tgaagctgcc
      781 ggaagcgcat taaaaggcga ccgcaaaagc tatcagatga acccaatgaa ccgtcgcgag
      841 gcgattcgtg aatcactgct ggatgaagcc cagggcgcag actgcctgat ggttaaacct
      901 gctggagcat acctcgacat cgtgcgtgag ctgcgtgaac gtactgaatt gccgattggc
      961 gcgtatcagg tgagcggtga gtacgcgatg attaagttcg ccgcgctggc gggtgctata
     1021 gatgaagaga aagtcgtgct cgaaagctta ggttcgatta agcgtgcggg tgcggatctg
     1081 attttcagct actttgcgct ggatttggct gagaagaaga ttctgcgtta atttttttcc
     1141 tctcagaaag ggggagttaa cctgttgaaa gtacggtgaa cgccccctca ccctaaccct
     1201 ctccccaagg gggcgaggga accgtccgca ctcgtatcac cccgcccgat aaaacggttt
     1261 atccccaagg atcgtcgccc gatgcataat ccgccgctgt ggcaggtaat cggcattggc
     1321 atagtgctga gtcacgcggt tatcccaaat cgcaatatca ttcggttgcc agcgccaacg
     1381 cacctgaaac tccggtttag tgatatgggc gaataaaaag cctaacaagg cttcgctctc
     1441 tttctcgctg acatcgacaa ttcgcgtagt aaagccttca ttcacaaaca gcgcctgttt
     1501 accgctcacc ggatgcgttc gcaccaccgg atgtagcaac ggaggatttt tcgcgaccgc
     1561 ctcgcgccag cgttgatgtt cctcctcggt tttgcggtat ttgtattccg ggaacgattt
     1621 acggaaatca tgctccgcac gcagcccgct cagcagctgg cggaagggaa cagagagcgc
     1681 ctcataggcc gcaataccgc tggtccagag cgtatcaccg ccggtcgaag gtaactcttt
     1741 agctgccaga atcgcccctg cgggtggcgt ttcaataaat gtcacatcgg tatgccagtt
     1801 gtcgttatcc ggcggattat cgttatgggt atccagcacg atgatctcgt caaccccttc
     1861 ggcatgaggg taaacagggt gaatatgcag ttcgccaaaa cgctgggcca gcgcgcgttg
     1921 ctgctgcggc gtaatagttt gatcgcgcag aaacaccacc tgatggcgca gcaccgcatg
     1981 gtaaagctgt tcaaactgat tatcgcttaa cgggcgcgtc aggtcggcac ccgaaatttg
     2041 tgcgccgata tacggcccca gcggggtaat actcagacgt tcactcattg tacttctcca
     2101 tgccagggcg tcagacggcg ctgtaacgcg cgcagaccca gttctaaaag aaaggcgata
     2161 atcgcaatca ccgcgatccc cgccagtacc acgtcagtcg cgagaaattc acccgctgac
     2221 tgaaccataa atcctaaacc gcgtgtcgcg gcaatcagct ccgccgctac cagcgtagac
     2281 cagcccacac ccagaccaat gcgtaatccg gtgaggattt ccggcagcgc gccgggcaaa
     2341 atgacaaacc acagcacctg cgcacggctg gcacccagcg actgggcggc acgaatgcga
     2401 acctgctgcg cgcttttcac ccccgccagc gccgacatcg ccaccggtgc aaaaatcgct
     2461 aaatagatca gtaagatctt cgaggtttca ccaataccaa accagatcac catcagcggc
     2521 aaataagcca gcggcggcac cggacgataa agttcgatta ccggatccag aatgccgcgc
     2581 accgtcgggc ttagccccat ggcgatcccg accggaatac cgatcaccac cgccgcgagt
     2641 agcgccagca caatgcgcgt caggctggct gccagatgct gccacagcgt ggcgtccata
     2701 aatccctgcg gtccggcaat ggtgagcagt tttgccagta cttgttgcgg cggcggcaaa
     2761 aacagcgggc taatcagttg cagcgccgcc accgtccacc atacagtgag taaaaccgct
     2821 aacgtgccaa tgcttaaggt cacctgacgc gagagcggcc agcgccattt cagccgccgc
     2881 gaatgcagtt tttcattaat gagcacactc atgagaacgc ctcccgttgc tcaaatacgc
     2941 ggcttaaaac atattcgcgc atggcgatga attgtggatc ggacttgatg ctgcggctcg
     3001 actctcccgc aacaaagcgg cgagcaaagt tgagcggcag ccgctccagc acgcggccag
     3061 ggccgggtga aagcagaacc agttcagtcg ccataaacac cgcttcttct atatcgtggg
     3121 taatcaacag cacctgcttg cccgtctcct gccagagttt cagcagcagg gtttgcatct
     3181 ggtcgcgggt gaaggcgtcc agcgcaccaa acggttcgtc gagtaataac agctggggat
     3241 tcgccgccag cgcgcgggca atccccaccc gctgacgttg accaccggaa agctgccaga
     3301 tatagcgttt ttccgcccct tcaaggccga cttttttcag catctggtgc gcgatttcca
     3361 gtcgctgcat tttctctata cctgccaatt gcaggccgaa cgccacgttg tcctgtacat
     3421 tgcgccacgg tagcagccct tcattctgaa aaactacgcc acgctctgct cccggtccct
     3481 caatacgctt acccgccagt agaatgctgc catgctgata aggcacaaaa ccggcaatca
     3541 gattcagcaa cgtggtttta ccgcagccgg acggccccag caccaccagt agctcgccgc
     3601 tttccagcgt caggttgata tcttccagtg ccggtttgcc gccataatcg gcgtaaagat
     3661 gagagatttg cagcatctgc gcctcctttt attgcacgaa gcgcgaggta acgtactggc
     3721 tgtaatcatt cgctacagcc gggaccttgc cctgctcttt caaaaactgc gcggtgtcga
     3781 tgatcgcttt gttcaccggt ccggtcagtt ctgccgtttg ttgctgcggc gtcagatagg
     3841 tattcccctt caccagcccc ggaacgtcac cttcaggcac gccgcttaaa cgcgccagtt
     3901 tgctgatgtt ttccggctgt ttcagccaca cgtctgggtt agcaatgtac ggttgctgag
     3961 catcgatggc gcttttagcg aacgctttca cgacctcagg atgtttctcg gcaaaatctt
     4021 tgcgcaccac ccagacgtcc agcgttggcg cgccccactg cccgacctgt tcagaatcgg
     4081 tcaacacctt gccgtctttt tccagggcgt taaccgccgg tgcccagaca taagcaccat
     4141 caatatctcc ccgctgccag gcagcgataa tcgcgggcgg ctgcaggttc acaatctcca
     4201 cttgcccggg tttaatgccc cagtgtttca gcgccgccag caggctgtag tgggtggtgg
     4261 agataaacgg tacggcgatg cgtttgccaa tcagatcttc cggtttgctg atagttttct
     4321 ttaccaccag cgcttcggag ttacccagtt ttgacgccag caagaagact tcaatcggca
     4381 cctgttggct ggctgcaacc gctaacgggc tggaaccgag gttgccgatt tgcacatcgc
     4441 ctgaagccag cgcccgcacg atgctggctc cgctgtcaaa cttacgccag tccacgctcg
     4501 ccccgctctc tttagcaaag gtgttgtcgg cctgagccac tttcgccggt tcggctgagg
     4561 tttgataggc cacggtgacg ttcaccgcct gtgcctgaaa agcgatgaat gccagtgcgg
     4621 ctagaagtgt gttacgcgat gaaattgcca tgattgtctg ctcccctgtc ttgttatggg
     4681 agcagtattc aggaataaaa acattcatta aaagaattag tcgttatcgc acagatgatt
     4741 ttattcttag caaaaaaacg gctaaggaaa gattaaatat gcaattactc cgttctaa
//
LOCUS       DAZFRN010000265         4679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFRN010000265 DAZFRN010000000
VERSION     DAZFRN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.267"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..415
                     /locus_tag="ACHKUB_004943"
     CDS             <1..415
                     /locus_tag="ACHKUB_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6657835.1"
                     /translation="IAFTCLSAFSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSAPWRL
                     KDISSRLYLSESLLKRKLKEEGVSFSKIILDERMQMAEYLLSTRCYPISKVAKVCGYA
                     SISYFTYVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            complement(457..909)
                     /locus_tag="ACHKUB_004944"
                     /pseudo
     CDS             complement(457..909)
                     /locus_tag="ACHKUB_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            973..1119
                     /locus_tag="ACHKUB_004945"
                     /pseudo
     CDS             973..1119
                     /locus_tag="ACHKUB_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1119..2276)
                     /gene="intS"
                     /locus_tag="ACHKUB_004946"
     CDS             complement(1119..2276)
                     /gene="intS"
                     /locus_tag="ACHKUB_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO6657836.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2438..2512)
                     /locus_tag="ACHKUB_004947"
     tRNA            complement(2438..2512)
                     /locus_tag="ACHKUB_004947"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2477..2479),aa:Arg,seq:cct)
     gene            complement(2588..3520)
                     /gene="yfdC"
                     /locus_tag="ACHKUB_004948"
     CDS             complement(2588..3520)
                     /gene="yfdC"
                     /locus_tag="ACHKUB_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO6657837.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3814..4569
                     /gene="mlaA"
                     /locus_tag="ACHKUB_004949"
     CDS             3814..4569
                     /gene="mlaA"
                     /locus_tag="ACHKUB_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO6657838.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1130 a         1118 c         1094 g         1337 t
ORIGIN      
        1 tattgctttt acatgtctct ctgcattttc ttctgaaaaa caatttggag tatttctgtt
       61 tggatgtttg aaaagcacag gggctaaagt taaaacgatt attcatacgg atttatctgc
      121 accatggcgt cttaaggata tatcatcaag attatatctc agcgaaagtt tactaaagag
      181 gaaattgaaa gaagaggggg tatcattcag taagatcata cttgatgaga ggatgcaaat
      241 ggctgaatat ttactcagca ctcgttgtta tcctattagt aaagtagcta aggtctgtgg
      301 ttatgccagt atctcatact ttacttatgt atttagacgt tattttggtg tttctccaag
      361 tcaatactct cagaggagtt cagaaagtaa aattcttact caccagggaa tctgaacatt
      421 gttcttgccc ccttatttcc agacaggggg tgtatcttaa gttaacgtta cccgctgacg
      481 tcgatattct cgcggagagc gataacccaa cgcactgtgc ggatggtttt cattgtaatg
      541 ttcgatcgcc actgcaagat tatgcaatgc cgttcttaca tttggtttcg gcatgaacgc
      601 gatgtagtct tccttcatcg ttttcacgaa cctttctgcc attccattac tctgaggact
      661 gctgattgcc gttgtgcagg gctccagatt caactctctg gcgaactgcc gtgtttcatg
      721 cgctctatac gctgaaccgt tatctgtcag ccactggatg gactgttccg gtactttatc
      781 gccaaagcgt ttttctatcg ctcctaacat gacatcctgc actgtcgctt tatcgtaacc
      841 tcccgtgctt gcggcccagt ctatggcttc acgatcgcaa cagtccaggg cgaacgtaac
      901 acgcagtttt gaatcgccac gggtttaaca gacacctcag agtcatttaa gatggcttaa
      961 agagaagtgc ccatgagcgg taagcgttat cccgaagagt ttaaaactga agcagtcaaa
     1021 caggttgttg atcgcggtta ttctgttgcc agcgttgcaa cacgtctcga tatcaccacc
     1081 cacagccttt atgcctggat aaagaagtac ggtccggatt actccacttt ttcatcaagc
     1141 caatccgccc accactgcat catttctctg cgcttatcga gatactgagc atggttgtaa
     1201 ataccgcgca cagatccgcc gttggcatgt gccagctgca cttcaatagc gtcagcaggc
     1261 cattcgtgct cgttcataat cgtgctgaat tcatgcctga atccgtgacc gctttccaga
     1321 ccttcatagc cgatttgttt gatcacaagc aataccgcgt tctcgcagat tggcttcttc
     1381 ttatcgttgc gcccggcaaa aacaaactct gagactggtt tggtgattga gcttagcgta
     1441 gtgagaagtt caaccacctg gtctgacatc gggaccacat gaattttgcg tcccttcatc
     1501 acactggcgt cgatggtgat aatcctgttt tcaaaatcga cgttcttcca tagcatggaa
     1561 cgaagctctt tcgttcttag ggctgtataa cgtaaaactt ttgtcgcaat gagcgatacg
     1621 atacttcctg aaaatgttgc cagtgctttg ttgaatgcag gcatctggtc tgctggaagg
     1681 aacgggaagt tcttcttgcg gtatcctttc atggcgtctg caaggtcagg tgccgggtta
     1741 tatttagccc ttccggtgac aatagcgtaa cgaaaaacct cgccgcatct tctgcgggct
     1801 ttgttggctc gctccattgc accgcgatct tcaaatctgc ggattacttc cagcagttgc
     1861 atcggctcaa tatcctgaat ctcaagaccg ccgatgatgg gtaaaatgtc gtcatcaaac
     1921 attttggcaa gttcagttgc atagcctact gaccagactt gcttcttgtg ctcgtaccat
     1981 tccttgtaaa tcgcactaaa tgaattgttg ttagacgaag cctttttcgc ctttaccgga
     2041 tcgatgccaa ccgagatgtc tttcctcgca gtccatgcct tatcccttgc ttcctgcaaa
     2101 gtcataagcg gatattttcc gacagtcagg attttctcct taccgtcaat cttgtagcga
     2161 agctgccata cctttttccc tgatacaggg acataaaggt acaggccatt accatcgagt
     2221 aggcggtatg gtttttcttt cggctttgct gcttcaatct gcttaacggt gagcatgggt
     2281 aaaaatccgg tgggtaaaat tattttatcc actttttacc cgtcatggag tgcggctgtc
     2341 aacgatctga cgcgaaccat gacgaaccgt gaatatacgg aaggcttgat attcagggga
     2401 ttttgcggac tggtacggat gggagcgaac tgataaatgg tgtcccctgc aggaatcgaa
     2461 cctgcaatta gcccttagga ggggctcgtt atatccattt aactaagagg acaatgcggc
     2521 atgagtatac ccgctaatgg actgcggggt aagtacgctg ccgctcgatt gcttaaaccc
     2581 tcgccattta tgctgggttt ttatcatttt tcttaatgtt ttccgcacgt tctgcttttt
     2641 ggcgtgcttc tgctttacgc ttgttgctca tgtcgttacg aatctgtgca tgactcatta
     2701 acgcgaagat aaaggtgccg ccgcagatgt tccccgctaa agtaggtagt gcgaagggcc
     2761 agatgaaatc gctccagtgc agcgtgccgt taaacaccag atagaggatt tcaacagaac
     2821 cgacaacgat gtgggtggtg tcacccagtg caataagcca ggtcatcaat ataatcacca
     2881 caatctttgc cgcacccgca gcaggaaaca tccaaaccat agtggcgatc agccagccgg
     2941 aaatgatcgc gttggcaaac atctcgctgg gggtgttctt catcacatcc atgccgattt
     3001 tgacaaatgc atcgcgagtt tcttcattga agataggcat atattcaaat gcccatgcag
     3061 caatacctgt cccgagaata ttacccagca gcacgacgcc ccataatcgc ataagtaagc
     3121 cgacgttgct cattgtcggt ttttgcatga cgggtagtac cgcagtcacg gtgttttcgg
     3181 taaataattg ctggcgggcc ataatgacga taataaaacc aaaggtataa ccgagattct
     3241 ccagtaagaa gctgcctggc actccttcca gttcgacatg aaatatccct tttgccagta
     3301 gcgaagcgcc catcgacaga cccgccgcaa tggctgacca cagtagcgcc attgcgtcgc
     3361 gttccagctc tttttcacca tcctggcgga tatgctcatg aattgccatc gcccgggagg
     3421 ggagtcgatc ttcatctatt tctatttttt tgccgcgctc tttttcttcg ctctcaactt
     3481 caatttcgtc gctgtgttga tcaattttgt cgttgtccat ggtctcttct cttgaattag
     3541 cacgtatagc taaagcgtag cggctttttt gctcgcaact ggcgggagtt actctgaaaa
     3601 tgtagaaaag gctgcgtttg cctttttctg tttctataga atcaagtagc ctacagggcg
     3661 gcgattacca ggctatgatc aaatcagcaa atcagggcgt ctggacatca gttgacgtgc
     3721 tgttacaatc gcccacacct aaacaggcgg atacggtatc gttccgtcat ggatggcaaa
     3781 ctgcataagc cataaaaaaa cagggagaca tttatgaagc ttcgcctgtc ggcgcttgct
     3841 ctgggaacta cgcttctggt ggggtgtgcg agttccggta cagatcagca agggcgttct
     3901 gacccgttag aagggttcaa ccgcaccatg tacaacttca acttcaatgt attagacccg
     3961 tatattgttc gaccggtcgc tgtcgcctgg cgtgattatg ttccgcaacc ggcgcgtaac
     4021 ggtttgagca actttactgg caaccttgaa gaacctgcgg tgatggttaa ctacttcttg
     4081 cagggcgacc cttatcaggg gatggtccac tttacccgct ttttcctgaa caccattttg
     4141 gggatgggcg gttttattga tgttgcaggg atggcgaacc cgaaactgca acggactgaa
     4201 cctcaccgct tcggtagtac gcttggtcat tatggcgtgg gttatgggcc ttatgtccag
     4261 ttaccgttct acggtagctt cacgctgcgt gatgacggtg gtgatatggc ggatggtctt
     4321 tacccggttc tttcctggct gacctggccg atgtctgtgg gtaaatggac gcttgaaggg
     4381 atagaaactc gtgcgcagtt gttggattcc gatggtctgc tgcgtcagtc gtccgatcct
     4441 tatattatgg tgcgcgaagc gtacttccag cgtcatgatt tcatcgctaa tggcggcgaa
     4501 ctcaaaccgc aggaaaatcc gaacgcgcaa gcgattcagg atgatttaaa agatatcgat
     4561 tctgaataag aaacaaataa aaaaggtgag tcgcaatact cacctttttt gttgttatcc
     4621 ctcaattacc acaagtaaaa ggagataaac cgacaatgac ccggacgtaa tgatgtaaa
//
LOCUS       DAZFRN010000266         4585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFRN010000266 DAZFRN010000000
VERSION     DAZFRN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.268"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..1424)
                     /locus_tag="ACHKUB_004950"
     CDS             complement(<1..1424)
                     /locus_tag="ACHKUB_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6657839.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNN"
     gene            complement(1756..2004)
                     /gene="ymgC"
                     /locus_tag="ACHKUB_004951"
     CDS             complement(1756..2004)
                     /gene="ymgC"
                     /locus_tag="ACHKUB_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO6657840.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(2117..2383)
                     /gene="ariR"
                     /locus_tag="ACHKUB_004952"
                     /gene_synonym="ymgB"
     CDS             complement(2117..2383)
                     /gene="ariR"
                     /locus_tag="ACHKUB_004952"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO6657841.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(2412..2684)
                     /locus_tag="ACHKUB_004953"
     CDS             complement(2412..2684)
                     /locus_tag="ACHKUB_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO6657842.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIISDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(2727..2963)
                     /gene="ycgZ"
                     /locus_tag="ACHKUB_004954"
     CDS             complement(2727..2963)
                     /gene="ycgZ"
                     /locus_tag="ACHKUB_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO6657843.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            3277..4488
                     /gene="bluF"
                     /locus_tag="ACHKUB_004955"
     CDS             3277..4488
                     /gene="bluF"
                     /locus_tag="ACHKUB_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO6657844.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT         1364 a          962 c          909 g         1350 t
ORIGIN      
        1 atattatttt ggttcagata gtcaagttgt tctttcgttt cgacaccttc agcgacgata
       61 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
      121 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
      181 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
      241 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
      301 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
      361 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
      421 aaaccttccg gcaatttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
      481 ctttgcgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
      541 gatatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
      601 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
      661 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
      721 gcggcgcagg cgattaacag aataaatatt aaaattccaa agcctttatc gataagtctg
      781 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
      841 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
      901 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
      961 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gttctgatag
     1021 agcaggatag gtaatctatt aacggtgtcg attgctggag ccaacagtaa attactgtcc
     1081 gggaaaacag gaatacgaga caacaggacc cgattcccag gcagggatgt acaccagact
     1141 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     1201 agctgatatt gctcctcgag gttgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     1261 gcttgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct cgctcccgcc
     1321 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     1381 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     1441 aagggtttaa tacatgaaaa tgccattcat tcgctcgcgc tggcatctac agagagcatg
     1501 gtagagagca taagaaatat ccacgccgaa gataaaattt tctccatctt gatctaaccc
     1561 attaattatt gagcataatg taggcagtac aaaataagtt aggcgggata tcaggcgtca
     1621 agaatggaga gcgagaactc tccattcttg acacctgata ttgcggacat aataagaaag
     1681 cataacgcct gaaatgctca ctttgcatca gcatggtgat acagctgatg tttattctaa
     1741 aaccttactc aagttctaag agagcacgga ttccctgtca ttaataatga tatccgttag
     1801 tgtagtctta tgcatttcaa tgtactgcaa tatttgcaga tagagatcag aaagaatcac
     1861 actggtttca tcttttaaac gcaagcgtaa agcctgatca ataatatgac tatcaacata
     1921 cccatgcgtc atgaacacct ccctctcgag atttttaaac aaagcacatt gaaaaataaa
     1981 tctctctggg attgaattat tcatgtcagc ctcgtagaaa tattaaccca ataaaattat
     2041 gctgtgtata gtctaaagcg acagacaaaa ataagatgaa tgagttttct ataaacttat
     2101 ccttaataat tagaagttac atatcatcag ctgtgtatcg caacacgatt tccagtgttt
     2161 ttctaatcac atcagctttg agaatatcac ttgttgtttc cagggagtgt atcagactta
     2221 agataatatt tttattatta acattatcgc ctgaaagcat taaattggtg acagcctgcc
     2281 ctaacactgc tgactcttct tctaacaaat tacccgaact gcgaaagtaa cttgctaacg
     2341 ctttatcagt tattgcatta tgaattgtag tatcttcaag catggtaacc tctcatctta
     2401 cttatgaaat tttaatgtat tctgtttatt ttcttaccat tgaacatact tttattgtca
     2461 gctgtcgctg ttcttcggtt tggccctcct gaatcactat tgctaaaatc actaattatg
     2521 ctgtcaatta aatcgcgtcg gaaagcatct tcctcagccg atttcattac gagtaattgc
     2581 ttaataagag tctgcaatga aaaatttatc ttcatgcgca gtatctggat caccgcctgg
     2641 ccagtaattt catattttat acgttcatta tcagatgtct tcataacgac ttcatagcta
     2701 acctgttaaa taatcaggct gttgcattat tcaaaaagca acccaattag tgcattataa
     2761 tgtttctgtt cctcttcccc agtcgcgtgt tccagtcggc aaagaagttt ggcacaaagc
     2821 gactttcgac tcagattacg tccatctttc aaaatctcag tcacaatttc cccgagagtt
     2881 tcctgctgag taggcaagtt agcttttgca aagtaacgag tgattgctcc cgcagaatct
     2941 aaagtcactg aattttgatg catgctacgc ctctgttaaa aatgttaatc aatgttaagt
     3001 gaggtaacaa tagcaaactt gtacgaaata tgtgtacaga atgataaatt aattttttgc
     3061 aattagtgct aatgcatata aaacaatgtg ttatgataaa acatattgca tcatttaact
     3121 gacaatctat acaagatttt tcgccaacta gtgttcaact ttttccattt tttatcaatg
     3181 taaagaaacg gttatgtttt ggtattaaaa gaaagacaca agaagatatg tctgttaccg
     3241 tcttactctc gcctcaccca ttaccctgga ttgttaatgc ttaccaccct tatttatcgt
     3301 agccatatac gtgacgacga acctgtcaaa aaaatcgaag aaatggtttc gatagcaaat
     3361 cgcaggaaca tgcagtctga cgtaacaggg atcttactgt ttaatggttc tcattttttc
     3421 cagcttctgg aaggtccgga agaacaggtt aaaatgatat atcgggctat atgccaggat
     3481 ccacggcact ataatattgt tgagctgctg tgcgattacg cgcctgctcg ccgttttggc
     3541 aaagcgggaa tggaattatt tgatttgcgc ctgcacgagc gagatgacgt tttacaggcc
     3601 gtattcgaca aaggcacatc aaaatttcag ctaacttatg atgacagagc gctacaattt
     3661 tttcgtactt ttgtccttgc aaccgaacaa tcaacctatt tcgagatccc tgccgaagac
     3721 tcctggcttt ttatcgctga cggatctgat aaagaacttg attcctgtgc cctttcacca
     3781 actataaacg accactttgc ctttcatcct attgtcgatc ccttatcgcg gcggataatc
     3841 gcttttgaag ccattgtgca aaaaaatgaa gatagcccat cagccatagc ggttgggcag
     3901 cgtaaagacg gggaaatcta cacagcggat ctcaaaagta aggcgcttgc attcacgatg
     3961 gcacacgcac ttgagctcgg tgataaaatg atttcaatca atctattacc tatgaccctg
     4021 gttaacgaac ctgacgcagt ctctttttta cttaatgaaa taaaggccaa tgctctggtg
     4081 cctgaacaaa tcatcgttga atttactgaa agtgaagtca tatctcggtt tgatgagttt
     4141 gccgaagcga ttaaatcgct aaaggctgct ggtatcagtg tagcaattga tcattttggc
     4201 gcaggttttg ctggtttgtt actcctgtca cgcttccagc ctgacagaat taaaatcagt
     4261 caggaattga ttaccaatgt tcataaaagt gggccacggc aggcaattat tcaggcgatc
     4321 ataaaatgct gtacatcact tgaaattcaa gtcagtgcta tgggcgtggc aacaccagaa
     4381 gagtggatgt ggcttgaatc cgcaggaatt gagatgtttc agggagatct gtttgctaaa
     4441 gctaaattga atggtatccc ctccatagcg tggccagaga aaaaataatt ttcagcacat
     4501 tctttcacat gatttcagta aatcatcgtg aaaatgaata cgttacgttt aaatttaatc
     4561 cacattaaaa ataatgtttc atcca
//
LOCUS       DAZFRN010000267         4326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFRN010000267 DAZFRN010000000
VERSION     DAZFRN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.270"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(32..817)
                     /gene="ygcW"
                     /locus_tag="ACHKUB_004956"
     CDS             complement(32..817)
                     /gene="ygcW"
                     /locus_tag="ACHKUB_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6657845.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            1136..2413
                     /gene="yqcE"
                     /locus_tag="ACHKUB_004957"
     CDS             1136..2413
                     /gene="yqcE"
                     /locus_tag="ACHKUB_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO6657846.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQFSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2440..3918
                     /gene="ygcE"
                     /locus_tag="ACHKUB_004958"
     CDS             2440..3918
                     /gene="ygcE"
                     /locus_tag="ACHKUB_004958"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO6657847.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTLAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         1163 a          974 c         1021 g         1168 t
ORIGIN      
        1 aataagagaa aacagaaacc accgttatgg attagcgcac taaataacca ccatcgacca
       61 ctaataaatg cccattgaca taatttgaag ccggacttgc gaggaataca gctgcgccca
      121 ttaaatcctg agtatcgccc caacggtttg ccggaatatg atcaagaacg cgctgattgg
      181 tttctggatt actgcgtgtc gccagcgtaa tatctgttgc ataatagcca ggggcaatac
      241 catttacctg aatattatat tgacctagtt catcacaata agctttggtg aatccggcaa
      301 gagcatgttt agtggcagaa tatgcaggtg accattgtcc acctaagtaa gagaacaatg
      361 aacagatatt aataattttg ccgcttttct gcgggatcat aatttttgcc gcttcatagc
      421 ttaactcgaa tgcggcggtc aggttcacat caatcatcgg atcccagtcg gcacgaccga
      481 agtccagcac cttattcagc ttacaaatac cggcattgtt taccagaata tcaactgtac
      541 cgaaacgctc acagcaagca gcgataatct tctgcggcgc gccttctgcg gtgatatcca
      601 cctgcatgaa gtccacctca acaccctgtt tttcaatcat ttcctttgtt tcgccgttat
      661 ctttgacgaa actaggaata aagatatttg cgccagcttt ggccaacgcc atggcaaatg
      721 cctggcctaa accgctattc ccaccggtaa caattgcggt tttacctttc agtgagaaaa
      781 aatccattga gaacgcattg agagattcga ttgacatagt tagctccaat ttccgtaagg
      841 caaaaaaaaa gaaaggtaac ttcccccgcg agagggaagt tacctttctc attatctctg
      901 gtaactggag ataattttta gcataacggc ataactgcta atgtgatcgt aatcacagtg
      961 tgatattcgt attgtcaaat agttttaaaa agcagaagcg tgattactct cacacaacac
     1021 ctctgctttc catgttattt cttattgcaa ttactagaga cctgagaaaa gtaatccacc
     1081 tgaaagggcg gcttactttt ctcttttttt tgtctattac aaatgggaat acgatatgca
     1141 acacaactca tatcgccgtt ggataaccct cgcgataatt agttttagcg gcggcgttag
     1201 tttcgacctg gcttatttac gttatattta tcaaattccc atggcgaaat ttatgggatt
     1261 cagcaatacc gagataggtt taataatgag tacctttggt attgcggcta ttattcttta
     1321 tgcccccagc ggcgttattg ccgataaatt ttcacaccgc aaaatgatta cttccgcgat
     1381 gatcattacc ggattactgg gtctgttaat ggcaacgtat ccaccgctgt gggtaatgct
     1441 ctgtattcag gtcgcctttg cgataacgac gattttaatg ctgtggtcgg tgtcgattaa
     1501 agccgcctcg ttgctgggcg atcatagtga gcaagggaaa attatgggct ggatggaagg
     1561 gctgcgcggc gtcggtgtaa tgtcgctggc ggtgtttacc atgtgggtct tttctcgctt
     1621 tgcaccggat gacagcgcca gcctgaaaac agtcattatc atctacagtg tggtttacat
     1681 cttgttgggg attctgtgct ggttttttgt tagcgataac aacaacctgc gcagtgccaa
     1741 taacgaagaa aaacagtcat tccagtttag cgacatcctt gccgttttgc gtatcagcac
     1801 cacctggtat tgcagcatgg tgatttttgg tgtcttcaca atctacgcca ttctgagtta
     1861 ctccaccaac tatctgaccg aaatgtacgg catgtcgctg gtggcagcga gctacatggg
     1921 gattgtgatc aacaaaatct tccgcgcgct gtgcggccca cttggcggca ttatcaccac
     1981 ctacagtaaa gtgaaatccc ctacccgcgt gatccaaatc ctttccgtac tcggcctgct
     2041 ggcgttaact gccctgctcg tcacgaactc taacccgcaa tcggtcgcga tggggattgg
     2101 cctgatttta ctgctgggat tcacctgtta cgcctcacgc gggctgtact gggcctgccc
     2161 tggcgaagcg agaacaccgt cttacattat gggcaccacg gtaggtattt gttcggtgat
     2221 tggattcctg ccggatgtct tcgtttaccc gattatcggc cactggcaag acaccctgcc
     2281 cgcagcagaa gcctaccgca atatgtggct gatgggaatg gcggcgcttg ccatggtgat
     2341 tgtctttacc tttttgctgt tccaaaaaat tcgtactgct gatagcgccc ccgcaatggc
     2401 tagcagcaag taagccagga aaattacagg agaaaaatca tgtcgaaaaa atacatcata
     2461 gggattgatg gcggaagtca gagcacaaaa gtggtgatgt acgatctgga aggtaacgtg
     2521 gtttgcgaag gcaaaggctt attacagccg atgcacacgc cagatgccga tactgcagaa
     2581 catcctgacg acgatttatg ggcatcatta tgttttgccg gtcacgattt gatgagtcag
     2641 tttgccggga ataaagaaga tattgtcggt attggtctgg gatccatccg ttgctgtcgt
     2701 gcgttattga aagccgatgg cacgcttgct gcgccgttga ttagctggca ggatgcacgc
     2761 gttacacgcc cttacgaaca cactaatcct gacgtggcat atgtcacctc tttttcgggt
     2821 tatctgacgc atcgcttaac cggcgagttt aaagacaata tcgccaacta ttttggtcag
     2881 tggccggtgg attataagag ctggacatgg agcgaagatg ctgcggtaat ggataagttt
     2941 aatatccccc gtcatatgct gtttgatgtg caaatgcctg gcaccgtcct cggacatatc
     3001 acaccacaag ccgcactggc gacacatttc ccggcaggac tgccggttgt ttgtaccacc
     3061 agtgataaac cggtagaagc tctgggggcc ggattactgg atgatgaaac tgcggtaatt
     3121 tctcttggca cttacatcgc attgatgatg aacggcaaag cactgccgaa agatccggtg
     3181 gcgtactggc cgattatgtc ttctattccg caaacattgc tgtatgaagg ttacggtatt
     3241 cgcaaaggta tgtggacggt gagctggctg cgtgacatgt taggcgagtc gttaattcag
     3301 gatgccaggg cgcaggatct ttcaccggaa gatttactca acaagaaagc ctctagtgtg
     3361 ccacctggct gtaatgggct gatgacggtg ctggactggc tgaccaatcc gtgggaaccg
     3421 tacaaacgcg ggattatgat cggctttgat tccagcatgg attacgcatg gatatatcgt
     3481 tcgatattgg aaagcgtggc gctaacgctg aagaacaatt acgacaatat gtgtaatgaa
     3541 atgaatcact ttgcgaagca tgtgatcatt actggcggcg gttcgaacag cgatctgttt
     3601 atgcaaattt ttgccgacgt gttcaacctt ccggcacgcc gtaacgccat taacggttgt
     3661 gcaagtctgg gagcagcgat taatacagcg gtaggtctgg ggctataccc ggattacgca
     3721 acggctgttg ataagatggt tcgcgtgaaa gatatcttta taccgattga gagcaatgcc
     3781 aaacgctacg acgcgatgaa taaaggcatt ttcaaagacc taaccaaaca tactgatgtg
     3841 atcctgaaaa aatcgtatga agtgatgcat ggggaattgg ggaatgtgga ttcgatccag
     3901 agctggtcga atgcgtaagt gaggaaggcc gggcgggaaa ctgcccggcc tgaacatacc
     3961 tgaatggtta tccccgctga cgcggggaac ataagttcga caacttaaaa aatgataaca
     4021 agtccggttt atccccgctg atgcagggaa cataataaaa actttaacta gttcggttta
     4081 tccctgtttt tacaaggaat attaactaca ctcgatgtct ttagaataaa agacaatacg
     4141 aatcgcactt tatcaatttt cacttaaagt atgaactgga tatctttatt cataagaact
     4201 attcatcaac agcgtataga ggcggttatg taaaaccact cattagcctc aaaacaaaga
     4261 caaacttcca gatttattac ttaaccaatg aataacaagc ttaaaaaaca tactgttatt
     4321 ctaaag
//
LOCUS       DAZFRN010000268         4320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFRN010000268 DAZFRN010000000
VERSION     DAZFRN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.271"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..1084)
                     /locus_tag="ACHKUB_004959"
     CDS             complement(<1..1084)
                     /locus_tag="ACHKUB_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO6657848.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDDSSWASPGTASASETSST
                     AVGAWARAGKAGTALGSASYAHAPFSTSVGMSAYVNPKASNGVAVGSWAKADALNSVA
                     LGTGAQATHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGLLSQRDVLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQ
                     EMATENRAAVSRNRQVGERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQ
                     SAALAGLFQPYSVGKFNATAAVGGYSD"
     gene            complement(1346..3214)
                     /locus_tag="ACHKUB_004960"
     CDS             complement(1346..3214)
                     /locus_tag="ACHKUB_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657849.1"
                     /translation="MGLFNYKSYASTQIRELVSGSYNLAIYSNTGKVLGLGSFLASRL
                     FAKHPDRVDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPCPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAVSNG
                     LSGEDVIVTGYSLGGGLTNIMARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDYEYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKG
                     DDSRERPVFIIGNDGNNLLAGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLS
                     DVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDH
                     RYLLKWRNKNVDYGKHTEGTNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGND
                     YLHGTSGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKS
                     EEDFLFLSKDIVTSTDEFISCLKQTGNNVEAGIAGGRIYFVNTALEDINHNSVYIV"
     gene            complement(3277..>4320)
                     /locus_tag="ACHKUB_004961"
     CDS             complement(3277..>4320)
                     /locus_tag="ACHKUB_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657850.1"
                     /translation="FNNAKVHLEKALNTVIQPNDDLYAVYKLAFGKPVGNPEESTTTW
                     REVLNQNGVSGFSVDKNYGGEFRYTYNGLEGSDIISTGYSIGGTARTMISRNDMVINT
                     NGGDDIIAVGMDVGRSYSAGYTDKKYLTSTGDGDDILIVGLGNNNVGIHLKDDASLRI
                     SDRDDVISDGELLLPSSSYDHGSGGIISSTTITMGDGDDTLLALGYEGFGNAIVNTDI
                     DLGAGNDTIHVNGDVKAVSIKGGSGMDILAISHGTLSTKDFSGFEVIDLGDNNGGVNI
                     IASDLVKSGVDAIEGGTLKIIGSSNSGVNLDGDDWKSDGVQSEDGVNYNVYTHEAAPD
                     IKILIDEHITNVM"
BASE COUNT         1259 a         1149 c          746 g         1166 t
ORIGIN      
        1 tgtcactgta cccgcccaca gccgctgtgg cgttaaattt acccacactg tatggctgga
       61 aaagaccggc cagcgcagct gactgggctg ctgcccgctt catttcctca tgattctcat
      121 taatctgacg ctgctgactg ttcatacgac gctcatgcgc ctgcagggta cgtgcctgag
      181 aagcaatggc gcgactgttg cgttcaccca cctgacggtt acggcttacc gcagcacggt
      241 tttctgtggc catttcctgt gtacgtacca gcccttcctg tacagtaatt cgttcaccgg
      301 ttccctcacc gcgcgtcaca taaccatgac tgtccacagc aacggtactt ccggtattta
      361 tctgctgttg ctggctgttc aggcgctctt catgcgtctc cagcacgtct ctctgggaaa
      421 gcaaccccct ggtgttacgg tttatggcca ccgtgttccg gataatattc tccctgttct
      481 ctgcagcaac cgtatcggta tggtcacgcg ccttaagtgc ttccgctgca atccgtccac
      541 tgacgtcact gtacacctca tccgccttcc ggttcagttg cccgacagtg gctgcatccg
      601 tatcccgtgt accatcactg acaccaccga gaacccgggt tttatccggt acacgcggac
      661 tggccgggtt tgtgacacct gagtcatatc cgatgtagac ttcattttca cgggttgtgc
      721 cggactgctg gccaagagcc actgaatatc tgtgcgttgc ctgcgcaccc gtacccagtg
      781 ccaccgaatt taaagcatca gcctttgccc agctacctac tgccacacca ttagacgcct
      841 tcggatttac gtaagctgac atccccacgg atgtggaaaa cggcgcatgc gcatacgatg
      901 cagagccaag agctgtccct gcctttccag ccctcgccca tgcacctaca gccgtggagc
      961 tggtctctga tgcagaggct gtgcccggag acgcccagga actgtcatcc tgcgcaaacg
     1021 ccggagcacc ggcaatcaga aaggaaataa gcgataaagt aataccgtgt cgatttattt
     1081 tcataaacaa cctcattgat atacacaata tactgacaca gagttaaatt aatatataat
     1141 caggagaaaa gtgaaagagc acaggtgcga aaatgaatta atctgagcgg atttaacaat
     1201 aaatgaagtg caatgaatta aaattaaaaa caaaaatcac ttgcagtata ttttttgcga
     1261 cacccatcgc aaccgcacac aaaaccaaac gcacaatgca ttgattttaa acaaaaaaac
     1321 aaaacagaaa ctcaacacat aaaaatcaga caatataaac agaattgtga tttatatcct
     1381 caagcgctgt attaacgaaa tatatccgtc ctcctgcaat acctgcctca acattatttc
     1441 cggtttgttt aagacatgaa ataaactcat ctgtcgatgt aacaatgtcc tttgataaaa
     1501 acagaaaatc ttcttcactc ttatgtttat taaaatccat gataaatgaa ttcccttcac
     1561 aagcagcatc aaagacgaag acatcatgac cagtaccacc atacagataa tcattacctc
     1621 ccccaccata taaccagtcg ttaccttctg ccccgtacaa ttcatcatta ccagaagtcc
     1681 catgaagata atcattcccg cgacctccgt gtaaaagact tactccttct ccccctgcac
     1741 tatgtaatat atcatttccg tcaagaccaa aaataatatt tccgaacaga ctatcatctc
     1801 cgtttgtacc ttctgtatgt tttccgtaat caacattttt attacgccac ttcagaagat
     1861 acctgtggtc tgtaatctgc tcttcttcta tctgataagg gcttagttta ctcctccagt
     1921 caccagaaaa agagatacct tcaatacctg ttgcctcagt cagaccggct ccccgttctg
     1981 aatggaaaaa cagcgtccca tcactgaccc tgtatatatt ccagtcatcc attacatcgc
     2041 taagaagcag cgtgtcatac ccactgccac catcaaccct gtttgctccc ttacctggtt
     2101 caatcttatc attccctcca gccccctcta tatagtcacc accctgcccg cctgccagca
     2161 gattattgcc atcattaccg atgatgaata caggtctttc acgactgtca tcccccttgt
     2221 catgaaccca tgaataccag gatgtccagc cactctgctc atccacaatg actgtactgt
     2281 ccggtgacat gtagtcgtaa aactgagacc ctaaaattct ttcgaaggcg tcagtttttg
     2341 aacccgcaaa atgcgcactc cagcttccca gcgggagtgc aagcacagaa aaggggataa
     2401 tattccagag gaatgaacca taaaggtctg tgtataaaac aatattatca ggagaagagt
     2461 catattcata atccggggtt gtcaagcccg gccccattgc tttaactgcc tcccagaacc
     2521 cggattcatc tcccagcaca cgaaataccg gatcgttttc aaaaccgata tttaatacct
     2581 tcccatcccc gtcaaatacc agcggggaag cccaggctat atagtcagaa ttagcataaa
     2641 aaccatcagc cagagactca ctgagtctgg ccataatgtt agtcaacccg ccccccaggc
     2701 tatagcctgt gacaataaca tcttcaccac ttaaaccatt actgactgca aagtctctga
     2761 tactttgaag caggttttct gcttcagaca gcgcttttcc attattgagc tgtgtataat
     2821 caacaatatc gcagatatca tttgttccac agaatgatat tgcaattttc tgtagattct
     2881 gttgagcatc aaacaaccca acaactttca actgaggacc aaatcctgac gcagggtttc
     2941 ctgtaaccgg acagggaaag cgataaaaac ctcttgaatc gacatattcc gaagataacc
     3001 ggagttcttc cactgtaata tcacgccagc cttcattaag aacatcggta gaataatcaa
     3061 ctctatctgg gtgctttgca aatagcctgg atgccagaaa acttcccaat ccaagaacct
     3121 ttccagtgtt ggaatatata gcaagattat aagagccaga aacaagctct ctaatttgtg
     3181 ttgacgcata agatttgtaa ttaaataacc ccatacaaac accttgatgt tgaaaaaact
     3241 ggatagaaag agccccatag gggctctttt ataagattac attacattcg taatgtgttc
     3301 atcgattaat attttaatgt ctggcgctgc ctcatgtgta tacacattat agttaacacc
     3361 atcttcagat tgaactccat cagacttcca gtcatctcca tctaagttta cacccgagtt
     3421 agatgaccca ataattttaa gtgtgcctcc ctctatagca tcaactccac ttttcacaag
     3481 gtcagatgca attatattca cccctccatt attatcacca aggtcaataa cttcaaaccc
     3541 agagaaatct ttagttgaaa gggttccatg actaatggcc agaatatcca taccagagcc
     3601 acctttaata cttaccgctt tcacatcacc attaacatga atagtgtcat ttcctgcacc
     3661 taagtcaata tcggtattaa caatagcatt accaaatccc tcatagccta aagcaagaag
     3721 tgtatcatca ccatcaccca tagttatggt ggtagacgat ataataccgc cactaccatg
     3781 atcatatgaa cttgatggta ataacagttc tccatcacta atcacatcat ctctatcact
     3841 aatacgtaaa cttgcatcat ctttcagatg tataccaaca ttattattac ctaggccgac
     3901 aatcagaatg tcatcaccat ctcccgttga tgtcaaatat tttttgtcag tatagccagc
     3961 actatagctt ctaccaacat ccataccaac agcaataata tcatcgccac cgttagtatt
     4021 aataaccatg tcattacgac ttatcattgt tctggcagtg ccaccaatag aatagcctgt
     4081 tgatatgata tcgctgccct ccaatccatt ataagtgtat cggaattctc caccataatt
     4141 cttatcaaca ctgaagccag aaacaccatt ttgattaaga acttcacgcc atgtagtagt
     4201 gctttcttct ggattaccta caggcttacc aaaagcaagt ttatatacag cataaaggtc
     4261 atcatttggc tgaatgacag tatttagtgc cttttccaaa tggacttttg cattattaaa
//
LOCUS       DAZFRN010000269         4267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFRN010000269 DAZFRN010000000
VERSION     DAZFRN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.272"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            144..4259
                     /gene="pic"
                     /locus_tag="ACHKUB_004962"
     CDS             144..4259
                     /gene="pic"
                     /locus_tag="ACHKUB_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFO6657851.1"
                     /translation="MNKVYSLKYCPVTGGLIVVSELASRVIKKTCRRLTHILLAGIPA
                     VYLYYPQISQAGIVRSDIAYQIYRDFAENKGLFVPGATDIPVYDKDGKLVGRLDKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYQSVSFGNGKNTYSLVDRNNHSSVDFHAPR
                     LNKLVTEVIPSAITSEGTKANAYKDTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDEQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLPPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIVLKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGTVVLNQ
                     QADTAGNVQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSRLLLDLKAQDTNVSVPIGSISPFGGTGTPGNLYSMILNGQTR
                     FYILKSASYGNTLWGNSLNDPAQWEFVGTDKNKAVQTVKDRILAGRAKQPVIFHGQLT
                     GNMDVTIPQLPGGRKVILDGSVNLPEGTLSEDSGTLIFQGHPVIHASVSGSAPVSLNQ
                     KDWENRQFIMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRVFVDKNDGTGNYV
                     ILEEGTSVPDTVNDRSQYEGNITLDHNSTLDIGSRFTGGIEAYDSAVSITSPDVLLTA
                     PGAFADSSLTVHDGGHLTALNGLFSDGHIQAGKNSKITLSGTPVKDTANQYAPAVYLT
                     DGYDLTGDNATLEITRGAHASGDIHASAASTVTIGSDTPAELASAETTASAFAGSLLE
                     GYNAAFNGAITGGRADVSMHNALWTLGGDSAIHTLTVRNSRISSEGDRTFRTLTVNKL
                     DATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMKDPAQGQSLNIPLVTAPAGTS
                     AEMFKAGTRMIGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYKN
                     FMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELDG
                     VDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDYT
                     GNFAGLGTKHYNTHSWYAGAETGYRYHLTEETFIEPQAELVYGAVSGKTFRWKDGDMD
                     LSMKNRDFSPLIGRTGIELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEKR
                     IKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
BASE COUNT         1163 a          977 c         1138 g          989 t
ORIGIN      
        1 ggagcggtaa ttttgtaagc aaacgaaaag tatttcacta tgtaacagac atcacaaaaa
       61 tacattaatg cagtcactat attttccgcc cgggtgataa cgtctggtta tcattagtac
      121 ttaaacatca tggagagtcc gcagtgaata aagtttattc tcttaaatat tgccctgtca
      181 ccgggggact tattgttgtc tctgaacttg ccagcagggt aataaaaaag acatgccgaa
      241 gattaacgca tattcttctg gctggcattc cagcagtcta tctgtattac cctcagatat
      301 cccaggcggg cattgtccgt tccgatattg cctatcagat ttatcgtgat ttcgctgaaa
      361 acaaagggct ttttgtacct ggtgccacag atattccggt atatgataag gacggaaaac
      421 ttgtggggag actggataaa gccccaatgg ccgatttcag cagtgtgagc tcaaatggcg
      481 ttgctacgct tgtatcacct cagtatatcg tcagcgtaaa gcataacgga ggatatcaga
      541 gtgtgagctt tggtaatggg aaaaatacat attcccttgt tgaccgtaat aaccactctt
      601 ctgttgactt ccatgctcca cgtctgaata aactggttac agaagttatt ccctcagcga
      661 taacatcaga aggaaccaaa gccaatgctt ataaagacac tgaacgttac accgcttttt
      721 atcgggtggg tagtggtacg cagtacacta aggacaagga cggaaattta gttaaggttg
      781 ccggcggata tgcttttaaa acaggaggaa ccacaggagt tcctctgata tctgatgcaa
      841 caatagtctc taatcccggg caaacctata atcctgtaaa cggaccttta cctgactatg
      901 gtgcccctgg ggacagtggt tctcctttgt ttgcttatga tgaacaacaa aaaaaatggg
      961 ttattgttgc tgtattaaga gcatatgcag gtattaatgg tgctacgaac tggtggaatg
     1021 tcataccaac agattatctg aaccaggtta tgcaggacga tttcgatgcc cccgtggact
     1081 ttgtttccgg actgcccccc ctgaactgga catacgacaa aacatcaggc acaggcaccc
     1141 tgagccaggg cagtaaaaac tggaccatgc acgggcagaa agacaatgat ctcaatgccg
     1201 gtaaaaatct ggtattcagc gggcagaacg gtgcaattgt cctgaaagac agtgtgactc
     1261 agggggccgg ttatctcgaa tttaaagaca gttacaccgt atctgctgaa tccggaaaaa
     1321 cctggacggg tgccggcatt attactgaca aggggacgaa tgtgacctgg aaggtcaacg
     1381 gggttgccgg tgacaacctg cataaattgg gggaaggaac cctgaccata aacggaacag
     1441 gtgtaaaccc gggaggactg aaaacgggag acggtaccgt tgtacttaac cagcaggcag
     1501 acactgcagg taatgttcag gccttcagtt ccgtgaacct cgccagcgga cgaccgaccg
     1561 tggtgctcgg agatgcccgt caggtcaatc cggataacat ttcatgggga taccggggag
     1621 gtaagcttga ccttaatggt aatgccgtta ccttcacccg actgcaggct gccgattacg
     1681 gggcggtgat tacaaataat gcacagcaaa aatcccggct tttactggat cttaaggctc
     1741 aggatacaaa tgtcagtgtt ccgattggca gtatatcccc ctttggcggt accggcacac
     1801 cgggaaacct atacagcatg atactcaacg gccagacccg cttctatatt ctgaaatctg
     1861 ccagctatgg taacaccctg tgggggaaca gcctgaatga cccggctcag tgggaatttg
     1921 ttggcacgga caaaaacaaa gcagttcaga cagtaaaaga ccggatcctg gccgggcggg
     1981 caaaacaacc cgttatcttt catggtcagc tgaccgggaa tatggatgtc accattccac
     2041 agctgccggg gggaagaaag gtcatccttg atggtagcgt gaacctgccg gaaggtaccc
     2101 tgagtgagga cagtggcacc ctgatattcc aggggcatcc ggttatccac gcctccgtca
     2161 gtggcagtgc gccggtcagc ctgaaccaga aagactggga aaaccgccag ttcataatga
     2221 aaacactgtc gctgaaagat gctgacttcc atctttcacg taacgcctcg ctgaacagtg
     2281 acattaagtc ggataacagc catatcacac tgggaagtga cagggtattt gtggataaaa
     2341 atgacggaac aggaaattat gtcattctgg aggaaggtac ctctgtcccg gacaccgtga
     2401 atgacaggag ccagtatgaa gggaatatta cgctggacca taactcaacc ctggatatcg
     2461 gcagccggtt caccggaggg attgaagctt atgacagtgc cgtcagtatc acctctccgg
     2521 acgtcctgtt aacagccccg ggtgcttttg ccgacagttc actgacagtg catgatggcg
     2581 gtcatcttac agcactgaac ggtcttttca gcgacgggca tattcaggcc ggtaagaaca
     2641 gcaaaatcac cctgagcggt acaccggtta aagatacggc taaccagtat gcccctgctg
     2701 tatatctgac ggacggatat gacctgaccg gcgataacgc aacactggaa attacccgtg
     2761 gagcacatgc ttccggtgat attcatgcct ctgcggcatc aacagttacc atcgggtctg
     2821 acacgccggc agaactggct tctgcggaaa cgactgcatc ggcgtttgcc ggcagtcttc
     2881 ttgagggcta taacgcagca ttcaatggtg ccataaccgg tggcagggct gatgtcagta
     2941 tgcataatgc actgtggact ctgggtgggg actccgccat ccacactctt accgtcagaa
     3001 acagccgtat cagttctgaa ggagaccgta ctttccgtac cctgacggtg aataaactgg
     3061 atgcaacagg cagtgatttt gttctgcgta cggacctgaa aaatgccgat aaaattaatg
     3121 tgactgaaaa agccacgggt tcagataaca gcctgaacgt cagctttatg aaggatccgg
     3181 ctcagggaca gtccctgaat attcctctgg tcacggcacc ggcgggaact tcagcagaga
     3241 tgtttaaggc cggcacccgg atgataggtt tcagtcgggt gactccaacc ctgcatgttg
     3301 acaccagtgg tggcaatacg aagtggatac tggacggttt taaagcggag gctgataaag
     3361 ccgctgccgc gaaggctgac agtttcatga atgccgggta taaaaacttc atgacggaag
     3421 tcaacaatct gaacaaacgt atgggtgacc tgcgtgacac aaacggtgat gccggagcct
     3481 gggcgcgcat catgagtggt gccggttctg cagacggtgg ttacagtgat aattacaccc
     3541 atgttcaggt cggctttgac aaaaaacatg aactggacgg agtggacctg tttaccggtg
     3601 tcacgatgac ctataccgac agcagtgcag acagccatgc gttcagcggc aagacgaaat
     3661 cggtgggggg cggtctgtat gcttcagcat tgtttgagtc cggtgcctat atcgatttga
     3721 ttggtaaata tattcaccat gacaatgatt acacaggtaa ctttgccggt ctgggaacga
     3781 aacactacaa cacccattcc tggtatgccg gtgctgaaac gggttaccgc tatcacctga
     3841 cagaggaaac gttcattgag ccgcaggctg aactggttta cggcgccgtg tccgggaaaa
     3901 cattccgctg gaaagacggt gatatggacc tgagcatgaa gaacagggac ttcagtccgc
     3961 tgattggaag aacagggatt gaactgggca agaccttcag tggtaaggac tggagtgtga
     4021 cggcccgtgc cgggaccagc tggcagtttg acctactgaa taatggtgag acggtactgc
     4081 gtgatgcgtc cggggagaaa cggataaaag gcgagaaaga cagccggatg ctgtttaatg
     4141 ttggtatgaa tgcgcagata aaggacaata tgcgctttgg tctggagttt gagaagtcag
     4201 cctttggtaa atataacgtg gataatgcgg taaacgcaaa tttccggtat atgttctgat
     4261 gtggggg
//
LOCUS       DAZFRN010000270         4219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFRN010000270 DAZFRN010000000
VERSION     DAZFRN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.273"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHKUB_004963"
     CDS             complement(<1..209)
                     /locus_tag="ACHKUB_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6657852.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHKUB_004964"
     tRNA            complement(324..399)
                     /locus_tag="ACHKUB_004964"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHKUB_004965"
     tRNA            complement(406..480)
                     /locus_tag="ACHKUB_004965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHKUB_004966"
     tRNA            complement(597..681)
                     /locus_tag="ACHKUB_004966"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHKUB_004967"
     tRNA            complement(690..765)
                     /locus_tag="ACHKUB_004967"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKUB_004968"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKUB_004968"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO6657853.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKUB_004969"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKUB_004969"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO6657854.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKUB_004970"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKUB_004970"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO6657855.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHYVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1094 a         1047 c          941 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt tcaggggagc taatgctccc
     2101 ctatcttatt tttccgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatccc caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgc tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtatgagca tggccgccaa cgtattacga tcgagattga ttcccgcttc ctgcagcgtg
     2401 atccaccctt gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggca
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtta gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttaa cacgaacttt atcagcaccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataacgggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc gggacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcaccag agtgaaattc gccattcgct aacagcgcga ttaatttcag cggaacggta
     3061 ttatccttca tgaaatcgtc tccactgcgc taacttcacc tgatgcacca ataaagcgaa
     3121 cttcgggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca taatgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gaggactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatctgcct ggggataatt tggtgctgtt ggaaattgtg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcacct
     3661 gcttgcctgt cgccagttca acacagtcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtaccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tttgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 cggggggatg gcttgtagat atgatgacag gaagagtttg tagaaacaca aaaaggccca
     4201 gtcttccgac tgagccttc
//
LOCUS       DAZFRN010000271         4185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFRN010000271 DAZFRN010000000
VERSION     DAZFRN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.274"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..1972)
                     /gene="speA"
                     /locus_tag="ACHKUB_004971"
     CDS             complement(<1..1972)
                     /gene="speA"
                     /locus_tag="ACHKUB_004971"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO6657856.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLED"
     gene            complement(1981..2112)
                     /gene="yqgB"
                     /locus_tag="ACHKUB_004972"
     CDS             complement(1981..2112)
                     /gene="yqgB"
                     /locus_tag="ACHKUB_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO6657857.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2206..2349)
                     /gene="yqgG"
                     /locus_tag="ACHKUB_004973"
     CDS             complement(2206..2349)
                     /gene="yqgG"
                     /locus_tag="ACHKUB_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO6657858.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2460..2711)
                     /gene="yqgD"
                     /locus_tag="ACHKUB_004974"
     CDS             complement(2460..2711)
                     /gene="yqgD"
                     /locus_tag="ACHKUB_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO6657859.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2767..3921
                     /gene="metK"
                     /locus_tag="ACHKUB_004975"
     CDS             2767..3921
                     /gene="metK"
                     /locus_tag="ACHKUB_004975"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO6657860.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1061 a         1135 c         1009 g          980 t
ORIGIN      
        1 catcttcaag ataggtataa ccgtacaaac ctgcctcgaa ctcttcaagg aactgttgtt
       61 gcagttcagc atcaagatcg gttttcttca cttgatcacg gaactgagtt aacagcgttt
      121 tcggatcgag ctgtacatat tgcagcatgt ccgccacggt atcgccttcg tcagacagtt
      181 ccacttcgac actaccgtca gggaacacga acacgtcaac cgcttcggta tcaccgaaca
      241 ggttgtgcat gttgccgagg atctcctgat atgcgccgac cataaagaaa ccgagcatcg
      301 gcggattctc tggatcgtac tccggcatag gcattgtcgt ggcaataccg tcaccatcaa
      361 tatagtggtc gatagcaccg tcagagtcac aggtaatatc cagcagcaca gcgcggcgtt
      421 ccggcacttg atccagccct tccagcggca gaaccgggaa caactggtcg atcccccacg
      481 catccggcat cgactggaac agcgagaagt tgacgtacat tttgtccgcc atacgttcct
      541 gcagctcgtc gataatcgga cgatgagcac ggttttgcgg atccagctgc ttttgcactt
      601 catggcacat gctcaaataa agctgctcag cccatgcgcg ttcttgcagg ctaaaggtgc
      661 cggaagagta gccgatatga atgtcgtgca gatccatctg actgtcgtgt aaccattcac
      721 gcagagaacg gcgagttccc ggttcgtgca tctcctgcca ggtttcccac atgctttgca
      781 gcgcacgcgg cgcatcttct gcaggcgcgg tcggcaccgt gtattcgtta cgttccacgc
      841 cgatgatatt agacaccagc acggtgtgat gcgcagtcac tgcacgaccc gattcagtga
      901 ttaccgtcgg atgcggcaga ccattttctt cacacgcatc gccaatcgcc cagatgatgt
      961 tgttggcgta ttcattgagg ccatagttta cggagcagtc agactgcgaa cgggtgcctt
     1021 catagtccac gcccagaccg ccgccgacgt cgaagcactg aatattgacg cccagcttgt
     1081 gcagttccac atagaaacgc gccgattcac gaacgcctgt ggcgatatcg cgaatattcg
     1141 ccatctgcga accgaggtgg aagtgcagta gttgcaggct gtcgagacgc ccggcttcac
     1201 gcagggtttc aaccagttgc agtacctgag tcgcagccag gccgaacttc gatttttccc
     1261 cgccggagga ctgccattta cccgagccct gcgaagccag acgtgcacgc acgcccagac
     1321 gaggaacgac attcagacgt tctgcttcat ccagcacaat ggcgatttct gacatcttct
     1381 caatgaccag atagaccttg tgccccatct tctcgccaat taatgccagg cggatatatt
     1441 cgcggtcttt ataaccgttg cagacgatga cgctacgggt catgccagca tgcgccagca
     1501 ccgccatcaa ctctgctttc gagcctgctt ccagccccaa cggttcgcca gaatgaatca
     1561 gggactcaat cacgcggcgg tgctggttaa ctttgatcgg ataaacaagg aagtaatcgc
     1621 cgttatagcc gtaggattcc cgcgcacgtt tgaacgcggc gttaatggaa cgcaaacggt
     1681 gctgcaggat ctgtgggaaa cagaacagtg caggcagacg ctgaccctgt gcttcacgag
     1741 ttttcactaa ctgcgcgaga tcgacgcgag cttccgggac gtccgggtcc gggcacacgc
     1801 tgatgtggcc cagctcgtta acgtcatagt agttattgcc ccaccaggca atattgtaag
     1861 tacgcagcat cttgctggct tcctgggagc tcattgcaac ctcctgcatg gagcgtagta
     1921 caccgtgttc gcccgctgac gaaggcaaac ccatagacat gtcgtcagac atagcgaacc
     1981 tcaaattatt ttattaagtg taaaacagtt aacgactatc gcagcctgga actgcgataa
     2041 caacccataa gcacatggat tttccagcag tgaatgctga cgctccaact gcgcaaccgg
     2101 tttctttttc ataacattat taagcacata accgaacgta agtgtgacag ttcggcgaaa
     2161 ccacgagaaa actcttgttt ttacaagagc gcccttgttc agtcctcagt aactgtaacc
     2221 agctcttgaa tcctgagaag cgccgagatg ggtataacat cggcaggtat gcaaagcaga
     2281 gatgcagagt gcggggaacg aatcttcacc agaacggtga gacaggttaa gcagcagaca
     2341 acggttcatt atttcgtatc acctccacgg ccgcctgtta agacgaaccc acaagccaaa
     2401 actctgattt caacccggct ggaagtggca acacgaaaga aacgtcgtgt gctttttatt
     2461 taagccgcgc gccgcgtttt ataccccaca atggcagaaa attgcaaaag ataaatacgc
     2521 agaatgccgg cattgtcagg aaaaatttcc agccacgttt ttaacagaat gagacacgat
     2581 tcaaaaaaaa gtggaaatcg ggtgaagaat tgacctaaaa tagccatcca gatgttaatc
     2641 catccatacc gattaacact cagactgcca gtgtttttaa cttgcagagt cgtggtagga
     2701 tccgctacca cagaaaatcc acacaacagt ttgagctaac caaattctct ttaggtgata
     2761 ttaaatatgg caaaacacct ttttacgtcc gagtccgtct ctgaagggca tcctgacaaa
     2821 attgctgacc aaatctctga tgccgtttta gacgcgatcc tcgaacagga tccgaaagca
     2881 cgcgttgctt gcgaaaccta cgtaaaaacc ggcatggttt tagttggcgg cgaaatcacc
     2941 accagcgcct gggtagacat cgaagagatc acccgtaaca ccgttcgcga aattggctat
     3001 gtgcattccg acatgggctt tgacgctaac tcctgtgcgg ttctgagcgc tatcggcaaa
     3061 cagtctcctg acatcaacca gggcgttgac cgtgccgatc cgctggaaca gggcgcgggt
     3121 gaccagggtc tgatgtttgg ctacgcaact aatgaaaccg acgtgctgat gccagcacct
     3181 atcacctatg cacaccgtct ggtacagcgt caggctgaag tgcgtaaaaa cggcactctg
     3241 ccgtggctgc gcccggacgc gaaaagccag gtgactttcc agtatgacga cggcaaaatc
     3301 gttggtatcg atgctgtcgt gctttccact cagcactctg aagagatcga ccagaaatcg
     3361 ctgcaagaag cggtaatgga agagatcatc aagccgattc tgcccgctga atggctgact
     3421 tctgccacca aattcttcat caacccgacc ggtcgttttg ttatcggtgg cccgatgggt
     3481 gactgcggtc tgactggtcg taaaattatc gttgatacct acggcggcat ggcacgtcac
     3541 ggtggcggag cattctctgg taaagatcca tcaaaagtgg accgttccgc tgcgtacgca
     3601 gcacgttatg ttgcaaaaaa catcgttgct gctggcctgg ccgatcgttg tgaaattcag
     3661 gtttcctacg caatcggcgt ggctgaaccg acctccatca tggtagaaac tttcggtact
     3721 gagaaagtgc cttctgaaca actgactctg ctggtacgtg agttcttcga cctgcgccca
     3781 tacggtctga ttcagatgct ggatctgctg cacccgatct acaaagaaac cgcagcatac
     3841 ggtcactttg gtcgtgaaca tttcccgtgg gaaaaaaccg acaaagcgca gctgctgcgc
     3901 gatgctgccg gtctgaagta atctttcttc acctgctttc aaaggccagc ctcgcgctgg
     3961 cctttttctt ttacctcacc gcggtggata ggcattcacg ccgcatccgg caaaaagctg
     4021 cccgcacaat aacatcattc ttcctcaaca cgttttatcg caaattatca tcactactga
     4081 aaccgattac accaataaca ccaaccaaat atttgtaata ttttcatatt attattcagt
     4141 tttcacagtt gttacatttc ttttcagtaa agtcttaatt gcaga
//
LOCUS       DAZFRN010000272         4131 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFRN010000272 DAZFRN010000000
VERSION     DAZFRN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.275"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            227..835
                     /locus_tag="ACHKUB_004976"
     CDS             227..835
                     /locus_tag="ACHKUB_004976"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO6657861.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="ACHKUB_004977"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="ACHKUB_004977"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO6657862.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4131
                     /gene="selB"
                     /locus_tag="ACHKUB_004978"
     CDS             2321..>4131
                     /gene="selB"
                     /locus_tag="ACHKUB_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO6657863.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
BASE COUNT          928 a         1044 c         1218 g          941 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtggcgc aatttaaccc
      361 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgttggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaagcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caaccttgga cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcaa ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg
     1381 tcaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg
     1441 tggtagtatc tcgcggcgaa ttggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggttgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gtttctccgg cgacaagttg ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgcgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggcg cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaagg ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg
     2701 ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt tgccggaacg
     2821 cgagcacgcc agccaacata gtttccgcct cgctattgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcgc aaaaccagcc
     3001 aacagaaacc gccaatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgtg ccgccagagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtca cgggacgcgt ttcactgctg gaagataacc ttgccgagct
     3241 ggtgttcgac acgccgctgt ggctggcgga taacgatcgc ttagtgctgc gcgatatctc
     3301 ggcccgcaac acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaagccg gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga
     3421 tgcgttatct gttcatctgg aacgcggcgc ggttaacctt gcggatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaatt gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggttg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt t
//
LOCUS       DAZFRN010000273         4099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFRN010000273 DAZFRN010000000
VERSION     DAZFRN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.276"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..603
                     /locus_tag="ACHKUB_004979"
     CDS             <1..603
                     /locus_tag="ACHKUB_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657864.1"
                     /translation="DFLNGDPDQPLIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            631..1092
                     /locus_tag="ACHKUB_004980"
     CDS             631..1092
                     /locus_tag="ACHKUB_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO6657865.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1112..3707
                     /locus_tag="ACHKUB_004981"
                     /pseudo
     CDS             1112..3707
                     /locus_tag="ACHKUB_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470092.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
     gene            3740..4000
                     /locus_tag="ACHKUB_004982"
     CDS             3740..4000
                     /locus_tag="ACHKUB_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657866.1"
                     /translation="MLEHFGAEASVLDMTIIVRSNPSKAAILEEFLHGTQEKLGIAEK
                     LGRYGLGSAETHVKDFMIRHKKMLGLSDEDVAILKILKDKGL"
BASE COUNT         1038 a         1068 c         1194 g          799 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg ctcattatgg ggcgcaccta ccaccaggaa
       61 aaccgcaccc ccggcagcct gccgggaaca aagacgcaga tgaccatccg ctccaaaacg
      121 tacatgggca gcggatttaa tgagctgaag tttgatgatg cgacagggag agaacaggtc
      181 tacatccacg cgcagaagaa catggatacc gaagtgctca acgaccgtac caccaccgta
      241 aaacacgatc accgcgaaac cgtaaaaaat gaccagacgg tcacgatcca ggaaggtaac
      301 cgccttctta cggtggaaaa aggccacaag atcaccggag tactgaaagg gtctttatct
      361 gaggatgtct ttcaggacag aggcacgatt gccggttcgg tgcatgttga cgctgtaaac
      421 aatggtggcg aaggcgacgg tatacaggct tatacggcga ttaaggaaat tttgctggcc
      481 gtggaggaaa gcaaaattgc gctgacgccg gatggcattc agctacaggt cggggaatcg
      541 acggtaatca ggctgtcgaa ggatggcatc accatcgtgg gcggttctgt tttcatcaac
      601 tgattcactc agattatcag ggagtatttt atgcagttca cttttaatga aggccacatt
      661 caactgccgt ctcaatggca ggatcagtca atgcaggtcc tggtgtccac agataacagt
      721 ggcatcaacc tggtcattac ccgcgaagcc gttccacagg gaaccctgac accagagctg
      781 taccaggaaa cgctggcgct gtaccagggc aaacttgatg ggtacaccga acatgcctgc
      841 cgggaaatca ctctggcaga ggccccggcc tggctgctgg attattcctg gaacggtccg
      901 gaagatgagg ggaatcaggg gcggattagc cagatagccg tattccagcg gcgtggagat
      961 acgctgctga ccttcacttt cagcacctcc ttatccctga agaacagtca aaagacgatg
     1021 ctgctggagg tgataaagag cttcaccccg ttgccaccgg agaatgacat ccaaaaggac
     1081 cagccgcgct gacataaacg agggataaca catgagtgaa ggaccaggcg ggccacaggg
     1141 agcgaccgca ggcggtacgc tggcaatgcg aatgctgtca cagcaggcga tggtcgccag
     1201 ccagatgaaa cgggcagcca acgacaaagc cattgcacag atgctggcag caaagaagtc
     1261 cggcccacct gccgccaggc tgggcgatga aattcagcat aaaagttttc tgggggcact
     1321 ggcaggggcc gtgctggggg cgatagtgac catcgcagaa ggttgcctga ttatggccgc
     1381 ctgcgccacc ggcccttatg cgctggttct ggtgcctgcg ctgatgtatg ccagctataa
     1441 ggcgagtgat tatgtggagg agaaacagaa ccagcttgag tcatggataa acagcttttg
     1501 tgacacggac ggcgccatca ataccggttc tgaaaatgta aacattaacg gaaagcccgc
     1561 tgccagggcc gccgtcaccc ttccccctcc tcccccacct ggagcaatac ctgaagtccc
     1621 acagggggaa ccctcatggg gtgatattgc cactgacctg cttgaatcgg cagcggaaaa
     1681 agcagtacca ctggcgaagg cctgggggaa cgctgttatc accctgacgg acagcaatgc
     1741 cggttttatg gatcgcgtca gcgccggcgc atcgcttctg tttcccgccg gtccggtatt
     1801 aatggagttt gccaccatgg tgggcgggcg tggcgaaatc aaaaaagatg tggatttccc
     1861 ggaagccggt gaggacacgg cgctctgcga caaggagaac aaaccaccga ggatagccca
     1921 gggcagcagc aacgtcttta tcaacaatca gcctgccgcg cgcaagggcg acaaactgga
     1981 gtgcagtgcg gcaatcgtgg aaggttcgcc ggacgtcttt attggcggtg agcaggtcac
     2041 atatctggat atccagctgg agttcccgcc atggcagaga atgatcctgg gaggaataac
     2101 gatagccagc tatcttctgc cgccagcagg actgctggga aaactgggga atctggcgag
     2161 actcggcaaa ctgggaaacc tgctggggaa aagcgggaag ctgctgggcg caaagctcgg
     2221 cgcgttgctg gggaaaacag gtaagtcgtt aaaaagtatt gccaataaag tcatcagatg
     2281 ggtaacagat cctgtcgatc cggtaaccgg cgcatactgc gacgaacgta ccgactttac
     2341 gctgggccag accctccccc tctccttcac ccgtttccac agttctgtac tgccgctgca
     2401 tggcctgacc ggcgtgggct ggagcgactc ctggagcgaa tacgcctggg tgcgtgaaca
     2461 gggaaaccgg gtggatatca tcacccaggg agccacgctg agatttgcct tcgacggtga
     2521 cagtgatacg gcggttaacc cgtatcacgc ccagtacatt ctgcgccgcc tcgacgatta
     2581 cctggagctg ttcgacaggg atgcactgag cagccgcttc ttttatgacg cctttccggg
     2641 aatgcgtctg cgccacccgg tgactgacga taccagcgat gaccgcctgg cacacagccc
     2701 cgacgaccgg atgtatatgc tgggcgggat gagcgacacc gccagcaacc gcatcacgtt
     2761 tgagcgcgac agccagtacc gcatcacggg tgtcagtcac accgacggga tccggcttaa
     2821 actgatgtac cacgccagcg actacctgaa agccattcac cgcacggata acggcataca
     2881 gacgctggcg acctacgaac aggatgcgcg ggggcggctg acagaagcgg atgcgcggct
     2941 ggactaccac ctgttttatg agtacgacgc tgcggaccgg atcatccgct ggtccgataa
     3001 cgaccagacg tggagccgtt tcacctacga tgcacagggc cggtgcgtga ccgtcaccgg
     3061 ggcggagggc tattacaacg ccacgctgga ctatggtgac ggctgcacca ccgtgacgga
     3121 cggcaagggc actcaccgtt attactatga tcctgacggc aatattctgc gggaagaagc
     3181 gccggacggc agtaccacca cgtatgaatg ggatgaattc catcacctgc tggcccgcca
     3241 ctcccctgcc gggcgggtgg agaagcgacc tgacgggccg tccgctgatg ctctttaaca
     3301 gtgaaggtaa aaccgtctgg cggccgggac agaccagcct gtgggggctg gcactcagcc
     3361 tgcccgcaga caccggctac ccggacccgc gcggggagtg ggacccggaa gccgaccccg
     3421 gcctgctgta tgcgggacag tggcaggatg cggaatccgg gctgtgctat aaccggttcc
     3481 ggtattacga gccggaaacc ggaatgtacc tggtgagtga tccgctgggg ttgctgggcg
     3541 gggagcagac ttaccggtat gtgccgaatc ctttaagatg gatagatccc ttaggattaa
     3601 ataaaggagc ttcattatct aaaatgatga atagctccag tgatctcatg gggttgagaa
     3661 ggcagcccca gaacttctgg cggctatatc gcggaaaaga catttaataa tagcattacc
     3721 aggctcagat gaattcagaa tgcttgaaca ttttggagcc gaagcttcgg tattagacat
     3781 gacgattatt gttcgttcaa atccgagtaa agccgctatt cttgaagagt ttttacatgg
     3841 tacacaagag aaactaggaa tagctgaaaa attaggacgt tatggactag ggagtgcaga
     3901 aacgcacgtt aaggatttca tgataagaca caaaaaaatg cttgggttat ctgatgagga
     3961 tgtagcaata ctcaaaatat tgaaagacaa ggggttgtga tgaatataat tctgccgata
     4021 aaagatatat ttattattgc gggaaacata gtttttagcg ttttatacaa tgaacatctt
     4081 gagttcccat gccgctgcc
//
LOCUS       DAZFRN010000274         4063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFRN010000274 DAZFRN010000000
VERSION     DAZFRN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.277"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..2146
                     /gene="sab"
                     /locus_tag="ACHKUB_004983"
     CDS             <1..2146
                     /gene="sab"
                     /locus_tag="ACHKUB_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin Sab"
                     /protein_id="HFO6657867.1"
                     /translation="IAVNAGKGGLTDAAGTTKTRLTYAPDGTLPAEELATMNDGLSFE
                     GDDGKIIHKKLNEKLTIKGGAGDNEDVTDSNIRVDANEKGDGLLVRMTRNLRDLASAV
                     FGTGGQITTVNDKGITITTPAAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALAD
                     AGGDVLNNAANIGDLKNALRDSSAALVNTGFGLQADDGSKVNTSLGKHITVGGDGQNI
                     STTVQNGSLTVRLADNIRLADDGSVSVGGTRMDKNGLTIKDGPSVTVNGIDAAGRVIS
                     GVKDGVNDQDAVNVSQLNREISQATAATTWALKTESGDEAVAVSSQTVEVRHGQNTRV
                     SAISKDDKGNYSYEIDVTGIPVEYTDSQGNPLVNIGGRFYTQTEDAATGKLTMTPAEP
                     ARVRISSEQPLVLTNVAAGNVSPVSTDAVNGSQLASAARIAGGDNVVWKDGKAALAPD
                     TFSELTTAGGGKTTVNGGVHKTPDNVADAVNMLNREGTKYFKAASSGQAAKAEGRDSI
                     AVGQGAVSRGQNSIAMGNGAEVTRQAAAGSVAVGSHARAGRANTGTYALNGQAVAGRT
                     GSDTAVVSFGQPGQERQLQSVAPGVLSANSTDAVNGSQLHATNRQVAGNTQAINTLGN
                     KFSQLSYRVEELNRDIRGVGASAAAMSGIPQAYLPGKSLMGLGVGGYGGESAIAIGVS
                     RISDNGKMIMKLNAGQNTRGNFSVGAGVGWQW"
     gene            2171..2944
                     /locus_tag="ACHKUB_004984"
     CDS             2171..2944
                     /locus_tag="ACHKUB_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6657868.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
     gene            3095..3920
                     /locus_tag="ACHKUB_004985"
                     /pseudo
     CDS             3095..3920
                     /locus_tag="ACHKUB_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1064 a          881 c         1209 g          909 t
ORIGIN      
        1 cattgctgtt aatgcaggca aaggtggatt aacagatgct gccggtacca cgaagacacg
       61 tctgacttat gctccagatg gaacattacc ggcagaagag ctggcgacca tgaatgacgg
      121 tctttctttt gagggtgatg acggaaagat tattcataaa aaactgaatg aaaaactgac
      181 catcaaaggt ggtgctggtg ataatgagga tgtaacagac agtaacattc gtgttgatgc
      241 aaatgaaaag ggagatggtc ttctcgtccg gatgaccaga aacctccgtg atttggccag
      301 cgcggttttt ggtacaggtg ggcagataac aacagttaat gataaaggca tcacaatcac
      361 aacacctgct gcgggcggtg gtcaggggaa tacggtttcc cttaccggct ccggcctgaa
      421 taacggtgga aatcagataa ccagtgtggc cagtggtctg aacggcactg cgcttgctga
      481 cgccggtgga gatgttctga ataatgcggc caatatcggt gacctgaaaa atgctctccg
      541 tgacagcagt gcggcactgg tgaataccgg ctttggtctg caggcggatg acggaagcaa
      601 ggtgaacacg tctctcggga agcatatcac ggttggtggt gacgggcaga atatcagcac
      661 gactgttcag aacgggagcc tgacggtcag actcgctgat aacattcgcc tggcagatga
      721 cggcagcgtt tctgtcggcg gaacccggat ggataaaaac gggctgacca tcaaggatgg
      781 cccgtcagtg actgtgaacg gtattgatgc ggctggccgg gttatttccg gcgtgaaaga
      841 cggggtgaat gaccaggacg ccgttaacgt ttcacaactg aaccgtgaaa tcagtcaggc
      901 aacggcagca acgacctggg cactgaagac tgaatccggt gatgaggctg ttgctgtttc
      961 ttcccagaca gttgaagtcc gccacggaca gaatacccgg gtttcagcca tcagtaagga
     1021 tgacaaggga aattacagct atgagattga tgtgacgggg attccggtgg agtacaccga
     1081 cagccagggg aacccgcttg tcaatattgg tggtcgtttt tacacacaga ccgaagacgc
     1141 tgcgaccggg aaactgacaa tgacgccggc tgaaccggcc cgtgtcagaa taagcagtga
     1201 gcagcctctt gttctgacaa atgtggcggc cggaaatgtg tcaccggtat ccacggatgc
     1261 ggttaacggg agccagctgg catctgcagc cagaattgct ggcggtgata atgttgtctg
     1321 gaaagacgga aaagcagcgc tggctcctga caccttcagt gagctgacaa ctgcaggtgg
     1381 cggtaagaca acggtgaatg gtggtgtaca taaaacaccg gataatgtgg ctgatgcagt
     1441 gaatatgctg aaccgtgaag gcacgaagta ttttaaagca gcctcttccg gtcaggcagc
     1501 aaaagcggaa ggacgtgaca gtattgctgt cggacagggg gccgtttccc ggggacaaaa
     1561 cagcattgcc atgggtaatg gtgctgaagt cacgcgtcag gctgctgccg gctctgttgc
     1621 agttggcagc catgcccgtg ctggccgcgc gaataccggg acttatgccc tgaacggtca
     1681 ggctgttgcg ggacgtaccg gaagtgatac tgcggtggtt tcttttggtc agccgggaca
     1741 ggagcgtcag ctgcagtcag tggcaccggg tgtgttgtct gcgaattcaa cggatgccgt
     1801 caatggcagt cagttgcatg caactaaccg tcaggttgcc ggaaatacac aggcgataaa
     1861 cactctcggg aataaattca gtcagctgag ctaccgggtg gaagagctta accgggatat
     1921 ccgtggtgtt ggcgcatcgg ctgcggccat gagcgggata cctcaggcct atctgccggg
     1981 taaaagtctg atggggctgg gtgtcggtgg atatggtggt gaaagcgcca ttgcaatagg
     2041 tgtgtcccga atctccgata acggtaagat gataatgaaa ctgaatgcag gtcagaacac
     2101 ccggggtaat ttctccgtcg gtgccggtgt gggctggcag tggtaacagg gaaaagcaag
     2161 gagaaatgta atgttattta gtcgaatgat gatgacgggt gtgctgtccc tgatgcttgc
     2221 aggatgtgga aatctgagtc atgtcacgga tgaaggaacg acggaagaag tggtatggcc
     2281 ttctccggag aaggtgatgt ttaatcatga cggaacacag aaaggttcgt ggccactgcc
     2341 agaaaagtta cgtcagataa aggcgggtat gaataaggac cagatttatc agctggcggg
     2401 gagaccacac tttgctgaag ggatgtctgg tgtaagggaa tgggattacc tgttcaactt
     2461 taccggaccg gagggggatt atacctgcca gtacaagata ctgtttgacc ggaacatgga
     2521 tgcccgctct ttctactgga agccggaaaa ctgtctgccg gaggcagaga cagcagaata
     2581 cagggagtca gtccccggtg atatattatt tgattttgat tcatggactc tgacagagag
     2641 ggggcgtgat gcactttccc gtgtggcaga gaagtttcag gcgggtcatg ttgtcggggt
     2701 gaagatagaa ggatttacgg accatcttgg cgggcaggcg tataaccggc tcctgtcaga
     2761 gaaacgggcg gaatcagtca ggggatggtt tattgcccgg ggattcaggg ctgaaaggat
     2821 acatgcaacg ggccgtggat atgatgcacc agtggtgttc tgcccggatg tggagggcga
     2881 agcactgaag gaatgtctgc gccccaatcg cagggttgaa ataaccggac agagcgttcg
     2941 ttaatatgca gtgtagcccc ggaatgtccg gggctttttt gtgcacagcc cccagctagg
     3001 ctctaggctg taatgtcgat cagttagtcg aacaatggcg acaactacct tgaaaaatgc
     3061 cgcgaatcgg tgaacgattc agatgcgatt atgtattgaa gcgatgtgtc gggtacttgg
     3121 tgtatacagg atcggttatt acgactgagt acagcatgac cctcagacag aaaacaaagt
     3181 gatgagcggc taaaactgga gattaaggtg gcacatatcc gcacacgcga aacatatgga
     3241 acctagctgc tccagacgga gctggcagat aacggaatta tcgtcggtcg tgacagactg
     3301 gcacgtcttc gtaaggagct gaggctgcac tgtaagcaga aacgcaagtt cagggcgaac
     3361 acgaactctg gtcacaatct gccggttgcg ccgaatctcc tgaatcagaa ctttacgcct
     3421 acagcaccta atcaggtctt ggttgcagat ataacgtatg ttgccccccg gaaggatggc
     3481 tgtatatcgc tggcgtcaaa gatgtttata cgagcaaaat tgtcggttac gccatgggag
     3541 agcgcatgac gaaatagttg acgggtaaag ccctgtttat ggcgctcagg aaccagcgct
     3601 cacctaccgg gctaatccac cactccgatc gtggttcaca gtactgcgca tacgattaca
     3661 gggccataca ggagcagttt ggtctgaaaa tatcaatgtc gcgtaaagtt aactgttacg
     3721 acaatgctcc gatggaaagc ttctggggaa cgctgaaaaa tgaaagtctg agccactatc
     3781 gttttaaaag ccgggatgag gccatctcag taatacggga atacattgag attttctaca
     3841 atcgtcagcg tcgtcactct cgtctgggga atatctcccc gacagccttc aggataaaat
     3901 attatcagat ggctgcttag aaaaagaaca aatggttatg tccgctattg cctgtacccc
     3961 tcactccggc gaaacaccag atgacttttc ctgccaggta aatagattct tctgaaaaat
     4021 tggagactaa tgtgaaatct ctggaataca ttaattttgt taa
//
LOCUS       DAZFRN010000275         3937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFRN010000275 DAZFRN010000000
VERSION     DAZFRN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.278"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            136..829
                     /locus_tag="ACHKUB_004986"
                     /pseudo
     CDS             136..829
                     /locus_tag="ACHKUB_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1052..1348
                     /locus_tag="ACHKUB_004987"
     CDS             1052..1348
                     /locus_tag="ACHKUB_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6657869.1"
                     /translation="MPDQSGYCWTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1656..1772
                     /locus_tag="ACHKUB_004988"
     CDS             1656..1772
                     /locus_tag="ACHKUB_004988"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657870.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1991..3424
                     /locus_tag="ACHKUB_004989"
     CDS             1991..3424
                     /locus_tag="ACHKUB_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO6657871.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     FSSACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLHKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDENLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNPALTVPVRLIPEELELFNWIKSYCIEKGIPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT         1185 a          751 c          868 g         1133 t
ORIGIN      
        1 aactggtttt catttttgaa tcaatatttt ctgaacaggg taatagtaca cgtattaaca
       61 gattatgtca gaaggccccg gtgatgcctt ctgacgatag attaatgtgg tattctattg
      121 gtatctggag accatatggc aacagttaca gtacattctc cccgctgtaa ttcctataaa
      181 gtgtaccatt atgatcacag cacctcagat catgagcgtt tccagtgcta atcctgcaaa
      241 cgtgtgtatt tcaactgcat tacatctacg aaacccgaaa accaggcatt aaagagcaaa
      301 ttgttgaaat ggttcataac ggagtcgggg ttcgcgatag ctccagaacg ctgaaggtgg
      361 atatcaacac cgtcattctc actttataaa acgctcacca cgcaaagtaa ccacatctcc
      421 agtcgccagt gccgatgtga cgcttatctg cgaacttgat gaacaatgga gttttgtcga
      481 aaataaagct cgtcagcagt ggcactggta tgcgtataac accaaatctg gaggtgtgct
      541 ggcttacact tttggtcctc gcactgatga aacatgtcgt gagttgccgg aactcctgaa
      601 gccattctcc acaggcatga taaccagaga caaccggagc agttatacca gagaaatgcc
      661 acaggataag catcttgtcg gcaagatatt tacccggcgc attgagcgca acaatctgac
      721 atttcgtact cacatcaaac gaccagcccg gaaaacgatt tgcttttcac gctccctgga
      781 aattcacgaa aagccattgg ttcatttgtc gaaaacactg tactactgac tggagtcatt
      841 accagggcct cttttgctgt tttttaccaa tgaatgataa tgagaatcga cagcgataag
      901 gagaaagtaa aataacaata atgctgttgt tttctatcgt attgattttt aattatttat
      961 tgtctttttg ctcagcccat ttttttacgc agacaaattt acccggggaa tacaaactgt
     1021 aacgacagtt caccacacta tgggggataa aatgccagat caatccggat actgctggac
     1081 tccttcacgc atcttacgga tgcctgaact gagccaattg ttaggtatat ctcgttcgac
     1141 aatttacgaa aaaatgaatc cgttatcgaa atactacgat gcaaccttcc ccaggccggt
     1201 acgtcttggt agtggttctg tgggctggcg ttcatcagct attgacgaat ggcttacttt
     1261 acatactgta cctgccagat cagcagtcaa aggtgttaat gacgaagtgc gacatacgct
     1321 ttcttcagat atgaccatga gcagataagt ttttgtcatc acgtgatcac ctcaggagga
     1381 aaaagaagca taagaacaat ttagccagaa gtttaattga taaacagaat ttccagcatt
     1441 cattgttgaa agagcagcta ttgtcgctat tgtagcggta attgctgcta taaccgaaac
     1501 gtattacgaa aaaggaagca tttatgtctt acatcaaacc ggatatgccg tattcagtat
     1561 atgcgtgacc atcaatgata gggaaggcaa taattaaagc tcaatacaat aaacaagtac
     1621 ctctgactct ggttgcaatg tcagtattaa cagcgatgtc aattgcctgt cagaatcagg
     1681 ttgacgtatg cagccccgga aatttacgtg gggccgttaa tatttacact atggttctgg
     1741 agaaagggaa aagaccgggg gaaaggtatt agtgaaataa tttcaggagg tattaagaat
     1801 gagaaatatg aatgatattt cgtggtaaat aatacagtct gcactgaatt aaccttatcg
     1861 gtttattaaa catcagatct acaaatgctc tgtatttctc agagcatcag gtctgaatag
     1921 cagctatttt cgctattgta gcgggatttg ctgctatttt actaacccat tactgaaaag
     1981 agaggctttt atgtgcttac ttgcaccaga aaatccttat ccgatatatg cactaccacc
     2041 gctggtgaga aatgcaataa ttgaaactca aaaaaataca caggcacctt tggctatggt
     2101 ggcgacatcc gcattaacag cgttctcaag tgcctgtcag aatcagattg acgtgtgcag
     2161 acctggaaat ttacgtgggc ctgttaatct ttactctctg attctggctg attccggtga
     2221 aaggaaaacg accgtggata aagtgtttat gaaagcattt tatctcaggg atgaagccct
     2281 ggcggaagaa tacgcgaaac tggttgagaa ttacagtaca gaaaaggaaa tatgggagca
     2341 aaagcaaaaa gcactggagt caaaattgca taaagaaatt cgcgccggta aagattgtaa
     2401 ggcaacagag tcagagctta aaagacatct gaataaatgt cctgttccgc cgcagatacg
     2461 ccggacaatt ttcaatgaga caacgataga gggaatgtta aaatattatt ccgacagcaa
     2521 tcgctctttt gctcttgtat ccagtgaagg aggaataatt tttgatggcc gggcaatgag
     2581 taaacttgga atacttaact ctttgtggga tggtggttct cttttcatcg acaggaaatc
     2641 atctcccgga attattttga aggatccaag actgacggta tcggtgatga ttcagcctga
     2701 tgtttaccag aaaggttttt gtacgcgaaa aaaagaactt gtgaaaacgt caggacatca
     2761 tgcaaggttt ttgatgtgtc aaccaacatc aacgcagggg acaaggataa taaccggaga
     2821 taattattca tcacagtatc aggatttgtt tgaacagagg atcaatgagc ttattgatga
     2881 aaatctggcg atgagtggtg aacgacgttg ccttcacttt tctcctcagg ctgccagaat
     2941 ctggacggat tattacaacg atgtggaatc gaagctgggg ggattggggc ctttaagaca
     3001 ttgccgggaa tatgctgcta aaaatgcaga gtatatggca agactggcag gacttattca
     3061 ccattcgagc ggtgaagagg gggacatttc cccttacact gcagaaatgg cgagagaatt
     3121 agcaatatgg tacggtaatg agtatgtgcg gttgtctaat ccgttaactt ttgacaaccc
     3181 tgccctgacc gtacctgtgc gactcattcc agaggagctt gaacttttca actggataaa
     3241 aagctattgc attgagaagg ggatcccctg tatgaaaaaa aatgatattt tacagcgtgg
     3301 tccgaaccgt ttccggaaaa aggataaaat caactggtta ctggatttac tgtatgaaca
     3361 aaacagagtt gtaccggtta ttgagggaaa aacgttgtgt gtcgcaccta actttgacct
     3421 ctgaacgact taatcgttga gtaacatata gtctcctctg ctacttgcgg aggagactaa
     3481 tgcctttacc acgtcagcgt tgttgtatta gaaaactctg gtacaggtca gtcagtaaat
     3541 ataactggtg taaccactat ttactattgg atagagtgtt gctcaggata ttgtcggctt
     3601 tagtctgagg tgaggtggga ccttcattgt tttcgtgttc aataaataat ttaggttaat
     3661 tagtattatt aatgggagtt atgttatctg ttatgttatc tgttatgtta tcttatctct
     3721 ctttctttcg taatatcact cctctttatg ctaagtattc tgtatgtgcg taatacatat
     3781 atctccatac actacagtgt ggtggtgtat ggagtttagt tatgtgtaaa ttatgttact
     3841 acactcatag cgtaatattg agaaagttaa tccactgaat gtatgtctgg tttgtattta
     3901 acactagtta cgtaccggaa cactattaga gtatatt
//
LOCUS       DAZFRN010000276         3896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFRN010000276 DAZFRN010000000
VERSION     DAZFRN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.279"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            47..1705
                     /gene="dpiB"
                     /locus_tag="ACHKUB_004990"
     CDS             47..1705
                     /gene="dpiB"
                     /locus_tag="ACHKUB_004990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO6657872.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1674..2354
                     /gene="dpiA"
                     /locus_tag="ACHKUB_004991"
                     /gene_synonym="citB"
     CDS             1674..2354
                     /gene="dpiA"
                     /locus_tag="ACHKUB_004991"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO6657873.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2395..3780)
                     /gene="dcuC"
                     /locus_tag="ACHKUB_004992"
     CDS             complement(2395..3780)
                     /gene="dcuC"
                     /locus_tag="ACHKUB_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO6657874.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1061 a          932 c         1012 g          891 t
ORIGIN      
        1 taattacatc cgtcaaagag gctcgggaca acccgtaagg aaaacaatgt tgcagcttaa
       61 cgagaataaa cagtttgcat ttttccaaag actggcattt ccgctgcgta tctttttgct
      121 gattctggtg ttctcaatat ttgtcattgc agccctggcg caatatttta cggccagttt
      181 tgaggactat ttaacgcttc atgtacgcga catggcaatg aatcaggcga aaattattgc
      241 ctccaatgac agtgtcatct ctgcggtgaa aacgcgtgac tacaaacggc tggcgaccat
      301 cgctaacaaa ttacaaagag ataccgattt tgattatgtg gtgattgggg accggcactc
      361 gatccgcctt taccatccta atccggagaa aattggttat cctatgcagt tcaccaaaca
      421 gggcgcgctg gagaaagggg agagctactt cattaccggg aaagggtcaa tggggatggc
      481 gatgcgcgcc aaaacgccaa tctttgatga cgatggaaaa gtcatcggcg tggtgtcgat
      541 tggctacctg gtgagtaaaa tcgatagctg gcgggctgag tttttattac cgatggcagg
      601 cgtgtttgtc gtgctgttag ggattctgat gttgctgtcg tggttcctgg cagcgcatat
      661 ccgtcggcaa atgatgggca tggagccaaa gcaaatcgca cgcgtggtcc gtcagcaaga
      721 ggcgctgttt agttcggttt atgaagggct gattgcggtg gatccgcatg gttacattac
      781 cgccatcaat cgtaacgcaa gaaagatgct ggggctgagc tctcccggac ggcaatggtt
      841 gggtaaaccc attgctgaag tggtcaggcc cgccgatttc tttaccgaac agattgatga
      901 aaaacgtcag gatgtggtgg ctaactttaa cggtctgagc gttattgcca atcgggaagc
      961 tattcgttca ggtgatgatt tgctgggggc cattatcagc tttcgtagta aagacgaaat
     1021 ttccaccctc aatgcgcaac tgacgcaaat aaaacaatac gtcgagagcc ttcgtacatt
     1081 gcgacacgag catctcaatt ggatgtcgac gctcaatggt ctgttgcaga tgaaagagta
     1141 tgatcgcgtg ctggcgatgg tgcaggggga gtctcaggcc cagcaacagc ttattgacag
     1201 cctgcgtgag gcgtttgccg atcgccaggt ggcggggctg ctttttggta aagtgcagcg
     1261 cgcccgggaa ctggggctaa aaatgatcat tgtccccggt agccagcttt cgcaactgcc
     1321 gccaggactg gatagcaccg agtttgcagc cattgtgggc aatttacttg ataacgcctt
     1381 cgaagccagc ctgcgtagcg atgaaggaaa caagatcgtt gaattattcc tcagcgatga
     1441 aggcgatgat gtggtgattg aagtcgccga tcagggctgc ggcgttccag agtctctacg
     1501 agacaaaata tttgagcagg gggtcagtac gcgtgctgac gagcccggtg aacatggcat
     1561 tgggttgtac ttgattgcca gctacgtaac gcgctgcggt ggtgttatca ctctcgaaga
     1621 taatgatccc tgcggtacct tgttttcaat ctatattccg aaagtgaaac ctaatgacag
     1681 ctccattaac cctattgatc gttgaggacg aaacgccgct ggcagagatg catgcggaat
     1741 atattcgtca cattcccgga ttcagtcaga tattactggc gggaaacctg gcgcaggccc
     1801 gaatgatgat cgagcgtttt aagccggggc taatcttgct cgataactat cttcctgacg
     1861 gcagagggat taatttattg catgaactgg tgcaggcgca ttatcccggc gacgtggtgt
     1921 ttaccactgc agccagcgat atggaaacgg tgtctgaagc cgtacgttgt ggtgtatttg
     1981 attatctcat taagcccatt gcctatgaac ggctggggca aacgctaacc cgtttccgcc
     2041 agcgtaaaca tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt
     2101 ttaatgccta tgctcgcggt gaacctaaag acgaactgcc gaccggcatt gatcccttga
     2161 cgctaaacgc ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg
     2221 tggcgcaggc actgaccatc agtcgcacca ctgccaggcg ttatcttgaa tattgcgcca
     2281 gccgccatct gattattgct gaaattgttc acggcaaagt tggcagaccg caacgcatat
     2341 accacagtgg gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt
     2401 gcctgtgacc gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac
     2461 caaaccaaca agcaccggta ccgaggtgcg ttttacgact tcaaacggcg agatcttcgc
     2521 catcccggca accgcaacga ctacgccaga aacgggcgaa agggtacggc ccaggtttga
     2581 cgcctgcagc atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt
     2641 cgggatcatc tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc
     2701 cagcatggtc agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt
     2761 agcgatagaa atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc
     2821 agcggcaacc agcagcatca ccacgttagc aaacgcatct gccattccgc gataagccac
     2881 ttccagacca gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc
     2941 aatcagcata caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc
     3001 aaaaatcagt acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg caggagcagt
     3061 ggtggtgatt tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata
     3121 acgttgccag aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag
     3181 cgtcgttttg aaggcgaagt caatcagcga catttcggaa gcttgcgccg ccagcaccac
     3241 atcccctgaa gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagcagctgc
     3301 gccacgactg ataccaacgt ttaccatcac cggaaatagg gttgccatca gcaaaacacc
     3361 cagaccggtt gcggaagaga cggccagaga catcagacag gcgacaaaat aggcggcaat
     3421 catcagcagg taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat
     3481 atcattcgcg ccgatatggg tcatgtaagc ggcaaatcca cacagcatca taatcatcat
     3541 gccgaggtcg ccgccgcggc tcattagtaa tattttaacg tattcaacga tatccgtggc
     3601 gctgtagcct gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat
     3661 aatcagcaat aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat
     3721 gtagcgagct acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat
     3781 aatttttcct gtctccaggc cccaaagtaa ataataaaaa attcctaaag cttaaggaaa
     3841 aaatatgccc aataaattgg cgatgaatgc tgattaaaat caagaaaaac tgccat
//
LOCUS       DAZFRN010000277         3820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFRN010000277 DAZFRN010000000
VERSION     DAZFRN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.280"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>3820
                     /locus_tag="ACHKUB_004993"
     CDS             <1..>3820
                     /locus_tag="ACHKUB_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFO6657875.1"
                     /translation="KPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPKKKDSPN
                     YGNPVNPVLGAKVLPGETDIALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEVSYSRSESLWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRFEYG
                     ERRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYRLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLTAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQTAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDLTATVSPDGKRSTI
                     EYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGDPAEQWQYDEHGWLTTISH
                     TSDGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTSTYTLT
                     GQLQSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDID
                     IPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGV
                     IRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTG
                     WMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHR
                     SLAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEA
                     EYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRLPG
                     QQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHV
                     NGDPSDFNQAV"
BASE COUNT          820 a         1044 c         1369 g          587 t
ORIGIN      
        1 aaaccggcgg cgcggcaggg cgacatgacc cgcaagggac tggatattgt gcagggttca
       61 gcaggggtgc tgataggcgc gccgacgggg gttgcctgct cggtgtgtcc taaaaaaaag
      121 gacagtccaa attatggcaa cccggtgaac ccggtgctgg gtgcaaaggt gctgccgggc
      181 gagacggaca ttgcgctgcc cggcccgctg ccgttcatcc tttcccgcgc ctacagcagt
      241 taccggacca ggacgcccgc gccggtaggg gtgtttggtc ccggctggaa agcgcccttc
      301 gatatccgct tacagatacg cgatgaaggc ctgatactca acgacagcgg cggcaggagc
      361 attcactttg agccgctgtt tcccggcgag gtaagctaca gccgcagcga gtcactctgg
      421 ctggcgcggg gcggagtagc ggcgcagcac agttcgcagc cgctaagcgc gctctggcag
      481 gtgctgccgg aagatgttcg cctgagtccg catgtgtacc tggcgacaaa cagccttcag
      541 gggccgtggt ggatactcag ctggccggag cgggtgccgg gggcggacga ggtgctgccg
      601 ccggagccgc ccgcataccg ggtgctgacg ggcgtggtgg acggcttcgg gcggacgctg
      661 gcctttcacc gggcggcgga gggtgacgtg gcgggcgcgg tgacgggggt gacggacggc
      721 gcgggccgcc gttttcacct ggtgctgacc acacaggcac agcgggcgga agtattccgt
      781 aaacagcgcg ccacgtcttt atcttcccct gccggtcccc gttctgcttc ctcttctctg
      841 gttttccccg acacgctgcc cgccggtaca ggatacggca cggataacgg tatccggctg
      901 gaggcggtgt ggctgacaca cgacccggca tacccggatg aacagccgac tgcgccgctg
      961 gcgcgctaca cgtacacggc cagcggagaa ctgcgggcgg tgtatgaccg cagcgggacg
     1021 caggttcgcg ggtttactta tgatgcggag cacgccgggc ggatggtggc gcaccattat
     1081 gcggggaggc cggagagctg ctaccggtat gatgataccg gtcgggtgac ggagcaggtc
     1141 aacccggagg ggctggacta ccgctttgag tacggagagc gccgggtaat catcacggac
     1201 agcctgaacc ggcgggaggt gctgtacacg gaaggcgagg gtggcctgaa gcgggtggtg
     1261 aagaaggagc atgccgacgg gagcatcacc cgcagcgagt atgatgaggc ggggaggctg
     1321 aaggcgcaga cggatgcggc ggggcggcgg acggagtacc ggctgcatat ggcgtcgggc
     1381 gcggtgacag cggtgacggg gccggacggc aggacggtgc ggtatggcta taacagccag
     1441 cggcaggtga cgtcagtgac gtacccggac gggcttcgca gcagccggga gtatgatgag
     1501 aggggaaggc tgacggcgga gacctcgcgc agcggtgaga caacgcggta cagctatgat
     1561 gacccggcga gtgagctgcc gacagggata caggacgcga cgggcagtac aaaacagatg
     1621 gcatggagcc gttacggtca actgctggcc ttcacggact gctcggggta cacgacgcgg
     1681 tatgagtacg accggtacgg tcaacaaacc gccgttcacc gggaagaagg catcagcact
     1741 tacagcagtt ataacccgcg cggtcaactg gtcagtcaga gggatgcgca ggggcgggag
     1801 acccgttatg agtacagcgc cgcaggcgac ctgacagcaa cggtatcacc ggacggtaaa
     1861 cgcagcacca tcgaatatga taaacgcggg cgtccggtga gtgtgacgga aggcggtctg
     1921 acgcgcagca tggggtatga cgctgccggg cgcatcactg tgctgaccaa cgagaacggc
     1981 agccagtcca cgttccggta tgacccggtc gacaggctga cggagcagcg cggctttgac
     2041 ggccggacgc aacgttacca gtatgacctg accggaaaac tcacgcagag tgaagatgag
     2101 gggcttatca ccctctggca ttacgatgcg tcggaccgta tcacccggcg cacggtgaac
     2161 ggcgacccgg cggaacagtg gcagtatgat gaacacgggt ggctaaccac catcagccat
     2221 accagtgacg gccaccgagt gtcggtccac tacggctatg acgataaagg ccgcctgacg
     2281 ggcgaacggc agacggtgga gaacccggag acgggggaga tgctgtggga gcatgagacg
     2341 gggcatgcgt acagcgaaca ggggctggcg acccgtcagg agccggacgg tctgccgccg
     2401 gtggagtggc tgacgtatgg cagcggttat cttgcgggga tgaagctggg cggaacaccg
     2461 ctggtcgagt acacgcggga ccggctgcac cgtgagacgg tgcgcagctt cggcagcatg
     2521 gcaggcagta atgcagcata tgaactgacc agcacataca ccctcacagg gcagctacag
     2581 agcaggcacc tgaacctccc gcagcttgac cgggactacg actggaacga caacggacag
     2641 ctaatccgca tcagcggccc gcaggagagc cgggagtacc gttacagtga cacgggaagg
     2701 ctgacgggcg tccacaccac ggcggcgaac ctggatatcg atatcccgta tgcgacggac
     2761 ccggcaggaa accggctgcc ggacccggaa ctgcacccgg acagcacgct cacggcgtgg
     2821 ccggataacc gcatcgcgga agatgcgcac tatgtctacc gctacgatga atacggcagg
     2881 ctggcggaga agacggaccg catcccggaa ggggtgatac ggatgcacga cgagcggacc
     2941 caccactacc actacgacag ccagcaccgt ctggtgttct acacgcggat acagcacggt
     3001 gagccgcagg tggagagccg ctacctctat gacccgctgg gccgccggac gggaaaacgg
     3061 gtgtggcggc gggagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa accggaggag
     3121 acctggtacg ggtgggacgg tgacaggctg accactgtac agacccaaca gacaagaatc
     3181 cagacggtat accagccggg aagcttcacg ccgctcctga gaatcgaaac agagaatggt
     3241 gaacaggcga aggcgcggca ccgtagcctg gcggaggtgt tgcaggagga cacgggtgtg
     3301 acgctaccgg cggagctggc ggtgatgctg gggaggctgg agcgggaact gcggcagggc
     3361 agcgtgagtg aagaaagcca gcagtggctt gcgcagtgcg ggctgacggc ggaacagatg
     3421 gccgcgcaac tggaggcgga atacatcccg gagaggaaac ttcatcttta ccactgtgac
     3481 caccgtgggc tgccgctggc gcttatcagc ccggaagggg aaacggcgtg gcagggggag
     3541 tatgacgagt ggggaaacct gctgggcgag gaaagcgcgc agcaccttca gcagtcactc
     3601 cgtctgccgg gacagcagta tgatgaggag tcggggctgt actacaaccg caaccggtac
     3661 tatgatccgt tgcaggggag atatatcacc caggacccga tagggctgag aggtgaatgg
     3721 aatctgtata aatatccatt aaaccctgtt cggtttatag attctttggg tttaaaattc
     3781 catgttaacg gtgatccaag tgattttaat caggctgttg
//
LOCUS       DAZFRN010000278         3785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFRN010000278 DAZFRN010000000
VERSION     DAZFRN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.281"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(130..555)
                     /locus_tag="ACHKUB_004994"
     CDS             complement(130..555)
                     /locus_tag="ACHKUB_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6657876.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLYALSEQQKAACVVLNPEKSHPYIEDMEMFLSPLPVVLEWHCMMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFQDCGGAST"
     gene            complement(552..935)
                     /locus_tag="ACHKUB_004995"
     CDS             complement(552..935)
                     /locus_tag="ACHKUB_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6657877.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASRHCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(1307..1906)
                     /locus_tag="ACHKUB_004996"
     CDS             complement(1307..1906)
                     /locus_tag="ACHKUB_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6657878.1"
                     /translation="MKTYNTNPGYEMDPQLLTHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEILRLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTGGDGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYALSYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLGCTLPETYSLV"
     gene            complement(2138..2287)
                     /locus_tag="ACHKUB_004997"
     CDS             complement(2138..2287)
                     /locus_tag="ACHKUB_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO6657879.1"
                     /translation="MEKVISHARYKLTPEEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
     gene            3353..>3785
                     /locus_tag="ACHKUB_004998"
     CDS             3353..>3785
                     /locus_tag="ACHKUB_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657880.1"
                     /translation="MTTRITFNMTSDETLRIVDEYCHTHKLSRSKVIDALLSATAPVL
                     NDINCYYQLAGKLQSRLLNGVYQRDLPHKRNVVSAEKYCLEIWENKLFTKRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRRIKDICEYQMQLSGEKA"
BASE COUNT         1075 a          886 c          863 g          961 t
ORIGIN      
        1 attcaggtaa gcaggactgc ggatatccgg tgtcgcgcct ttgtgtcacg aacggggtgg
       61 gtgcgaaaca ccagataaaa tgcaggttcc gatacctctg aacgccctga gcaggtcggg
      121 catttcggat taagtactcg cacctccgca gtcctgaaac aagtctggct ggtagctgta
      181 aacagacttc gtacataccg ctctgcaata aatccccgta ccacaggcct cacagaattt
      241 cttcccatga aaaaactgct cacacatcat acaatgccac tccagcacga ccggtaatgg
      301 cgataaaaac atctccatat cctcaatgta agggtgggac ttttccggat tcagcaccac
      361 gcaggctgcc ttctgttgct cgctcagggc atataaatca tgctcaaacc atgccccctg
      421 agcatctgtc tgcaaaatca accggccatg acaggaaagg cagaaacact gccggatatt
      481 tctgctgagt gcagaatcag cactgattat gtgttcaccc ggcgtgatcc ccagccccgt
      541 ttttataccg ttcattctgc caccccctcc tcactgaagt gcccggtatg gcagtgcgtg
      601 cagtaccgct ccccataata atcgtgatga cattgtctgc agtgccggct ggctttacgc
      661 accacgggta aggcatccgg tacgaattgc tgcagacgct taatcagttg tatctccctg
      721 cgttccggtc tgacataagg gcactgctga ccgtgctccg tcagcccgtc gtcagtgtgt
      781 tcaaaccagg gacgctcagt gtcgtattgc gggtggtatc tgagcgcact gccgcaaagg
      841 tggcaagtgt agcgatcgta aggtgcagtc tgtgcggtac gggcacccgt cagacgcccg
      901 ttgccatcaa atgcaataaa ggattttgcg tacatcgtca tattcctcag ccgccagacg
      961 acacgcaggc aacagctgtc ccggtacggg cagtgtggtc agggggtgaa tggcggttca
     1021 gttaaaatct ggactggagt ttgggtaaag caggctggtt gctgttacag cactgtggtt
     1081 gtcacacaat ggcccaccgg cgtaaaaaac tgtcagtagg cagggatgga catcggggga
     1141 tgcgctgtat cggttgccgg aaggaatggt aaagaatgtg ccatggttaa atctccgtat
     1201 ggttcatacg gaatagccat gtcgtaaaaa atgcgcagag tccctgaagt ggcctccgga
     1261 accgaccgta acaattagcg ccagcccgta gttgtcgttc agggtcttac accagagaat
     1321 aagtctccgg cagggtgcag cccagtcgcg gtcgaccccg gttacttttt tcaggaagcc
     1381 cgctgcattt caggatgatg ccacattctt tctgttctcg tttggccagg tagctgaggg
     1441 catagcgtaa ggcattcacc gctttgtgat ctgaatagtg aatgacgtgg ttaatgttta
     1501 ccggataatt tttattaaac cggcaccagt ggtagtaacc atcccccccg gtaacccgcc
     1561 tccagatatc tcccatcaat ctggatacca gatacgaact cctgtggctc tgaccgttca
     1621 gatagcccac aaaatgaatg tgaagccctt tacgttccgt atattccatt acccaggcat
     1681 acccgaccag tttctctccc acttcgttaa tcagtcgcag tatctccgca cacatggcgt
     1741 acctgcaggc acacctgtag ctgagggtgt tttttcggta agcaaaatcc attctgaacg
     1801 gtaacagttt cgaatatacc ccgaataatg aatccagatg ctggttaaag tgtgtcagga
     1861 gctggggatc catttcgtaa ccgggattgg tgttgtaagt cttcatgttt cttcctcgtg
     1921 ttctgtaaca caggaagaag cgctggcgta atttttgaag aaaacgttat cacacgaaga
     1981 cgtgcaggtt cacaactccc cgggcgccac agagaaagtg ggaccacacc cgggtatcag
     2041 aaccatacag agaacggcat acgtgacagt caatcacagc gcagataata tctgaagtga
     2101 tgtcattgtg acgactgtac ttcatcagaa acatttatca ggtaacatat ttccagactg
     2161 aagcgggaac acgatcgtaa agtttgttca tggtcagttc tgccagccgg tgatcgaccg
     2221 cagagttgaa ggcttccatc tcctctggag taagcttata acgcgcgtgg gaaatcactt
     2281 tttccagtga acaacagtgg aactactctg gtttaaaatt ccattaccct ctcgttatct
     2341 tgtactgtga ttaacgtaat atcgtcataa tggcctgacg ttatcccgaa agcctttcca
     2401 cttccctgtt tctccggtaa aagatgtttt taatactgaa ggcataacgc cataaccaga
     2461 tattacaatt aacgtagctt tccgcaacgt actacatgag taatagaggt acagggttaa
     2521 acaacactcc ggaagacttt ttaatgacat gaggcatact cttttacgta ttactaccaa
     2581 aactcatcag gtgagctgca ggaacagatc attaacagta attccttaat caccgttatc
     2641 cacagtatca acactggcga agtcatactc ttagctataa aggtgatgac gggttcagaa
     2701 tagttattac accatacaac aaaatacaga aaacaacagg aaaaataaca gaaaaaaata
     2761 gcagacatta cttaacaaag cacgttacag tcagtgtgtg aacattacag atttatattc
     2821 aggtgtggcg ggactacatt actgaagaga gagttaaggg cagagttaca agtactacta
     2881 cagagaatat cgctgcacag gaatattgtt cgatgagaca gacttctgca ggacttggtt
     2941 acaaggtgta tttacgaggt agaattacag gatagaatta caaggtacaa tgacagagtt
     3001 catttacacg gtagaattac ataaacatac ttctataaat aatactaatt aacctttatt
     3061 attttttaat ggtacatgaa ctgtttttag ctgtaacgat aaacaaatcg cccaaaacga
     3121 aaaatactgg ttatcattgc tcagaaaact cgtacgagtg gcagcttccc ccctgagcaa
     3181 tgcttcattt tcggtggttc tgtgtgatag agttatgctg actacagtgg cagtaacact
     3241 actttccacc acacgaggat aaaaccagtc aaaaaatcaa ttatatgaat taaaagtatt
     3301 ttttatgaaa tgttatgcat atcaaccagt atgattaaca gaaaatttca tcatgacaac
     3361 acgtataact ttcaatatga ccagtgatga gacgctgcga attgtggatg agtactgtca
     3421 tacccacaaa ctttcgcgct ctaaggtaat cgacgcatta ctcagcgcca cagcacctgt
     3481 ccttaacgat attaactgtt attatcaact tgcaggcaaa ctgcagtcac gcttattaaa
     3541 tggagtgtat caacgggatc ttcctcacaa acgcaatgtc gtcagtgcag agaagtattg
     3601 tctggagatc tgggaaaaca aacttttcac caaaagaatc ctggagtttg acagttcaaa
     3661 tggtgttctg tacgcgctga aacataaaag acactatcgc cgggataaga tgattggacg
     3721 ggttgaaagc cggcgtatca aagacatttg cgaataccag atgcaactgt ccggggagaa
     3781 ggcaa
//
LOCUS       DAZFRN010000279         3779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFRN010000279 DAZFRN010000000
VERSION     DAZFRN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.282"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            7..1254
                     /locus_tag="ACHKUB_004999"
     CDS             7..1254
                     /locus_tag="ACHKUB_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO6657881.1"
                     /translation="MKRKHLLLLLLFSFSTNSAPLYSLIREAVMHDPIVMEARAELTS
                     AQSRIEQASSAHWPVVTATGSKLLSQSHRYSYDYDTEDILPGIRGEVNIFASGAIEAD
                     VRRSESEAEYYHYKMEETKEETIHSFVSLYLDALREKQSIAVLEQSLSRHNAILNDLN
                     TISIHDTGRESELVQAEARRLMVRQQINSRSRVLKTTLGKLSTWTKNPVTEADLENPF
                     SRMTEAKLLTDFTQAPQKGNPSWLASQADVESKKAALKAQELARYPRVDLTGSVTRDD
                     QQIGVNLSWDLFNRNASYGVTEKAAQIVAATGRLDSVARMIDETGRLSLITVRQSRGE
                     METLRRQEQASARVVDFYRLQFQVARKTLIELLNAENELYSVGLSRVQTEDQMLHGML
                     DYLYSQGMLLKWSGVNLSGEEEK"
     gene            1279..3426
                     /locus_tag="ACHKUB_005000"
     CDS             1279..3426
                     /locus_tag="ACHKUB_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO6657882.1"
                     /translation="MKSIFEGLSQITALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRSPEDIPSLAVPALLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDALMAVYSGYCWFVQPQAKQDRRSELPEYDMSGAWFWRVLWRFRAYYGQVIVA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVITAILFECAAKLIRSYLTDV
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFISVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIISFSTAIQQINTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNEI
                     VRRPVERESQTHYLTVKDIQGEFHFSAVTFFYKPELPAALKGMSVCIRPGEKVGIIGT
                     IGCGKSTLLKMMVGLYRQNKGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPCGLDMQIGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTENIVLKGLNHWSRGRTMVVVTHRLQVLNIVDRIIV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3487..>3779
                     /locus_tag="ACHKUB_005001"
     CDS             3487..>3779
                     /locus_tag="ACHKUB_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin secretion protein D"
                     /protein_id="HFO6657883.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKTLEVREGD"
BASE COUNT          951 a          788 c         1031 g         1009 t
ORIGIN      
        1 ataaaaatga agcgtaaaca tttgttatta ttattgttgt tttcattttc cactaacagt
       61 gcgcctcttt actccttaat tagggaggca gttatgcacg atcccatagt aatggaagcc
      121 cgggcggagt taacttcggc acaatcccgc atagagcagg caagctctgc acattggcca
      181 gttgtcacag ctacaggaag taaactcctt tcacaaagtc accgttattc ctacgattat
      241 gacactgaag atattttacc cggtattcgt ggtgaagtga atatatttgc ttcaggggct
      301 attgaggcgg atgtgcgtcg gagtgagtca gaagccgaat attatcatta taaaatggaa
      361 gaaacaaaag aggaaacaat tcactctttt gtttcattat atcttgatgc actcagggaa
      421 aaacaatcca ttgcggtact tgaacagagc ctttcccggc ataacgcaat tcttaatgac
      481 ctgaatacca tcagtattca tgataccggg cgggagtctg agcttgttca ggccgaagcc
      541 agaaggttga tggttcggca gcagataaat tctaggagca gagtacttaa aaccacgctg
      601 ggaaaactgt ccacttggac aaaaaatccg gtaaccgaag ctgatcttga aaatcctttt
      661 tctaggatga cagaggccaa attattaact gattttacac aggctccaca gaaaggtaac
      721 ccgtcgtggc ttgccagcca agctgatgtt gagagtaaaa aagcggcact gaaagcacag
      781 gagcttgccc ggtaccctcg ggtggattta acggggtctg taacccggga tgaccagcag
      841 ataggtgtca atctgtcttg ggacctcttt aaccgtaatg ccagttatgg tgttacagaa
      901 aaagctgcgc aaatagtggc agctaccgga cgactggact ctgtcgcccg aatgattgat
      961 gaaaccgggc gattatctct gataacagtc agacaaagtc gaggggaaat ggaaacgctc
     1021 agacgtcagg aacaggcttc agccagagtt gtggactttt atcgtcttca gtttcaggtg
     1081 gcaagaaaaa cactgattga attactgaat gctgaaaacg aactgtacag tgtcggactc
     1141 tcccgggttc agacggagga tcagatgctc cacggtatgc tggattatct gtattcccag
     1201 ggaatgctcc tgaaatggag cggagtgaat ctttctggtg aagaagaaaa ataacggggt
     1261 tgaagggttg gtaattaagt gaagagtatt tttgaaggat tatcacagat aacggcgtta
     1321 ctgggtacac ctgtatctgc tgagactctg gctgccggta ctgtccgtac agatgtatcc
     1381 gggattgatt ttcgttctgt tggcgagttt cttcgtagtg aaggatttga taatcatctt
     1441 tcccggcgat ctccggaaga tatcccgtca cttgcggttc cggcgcttct tttattgaac
     1501 gctcaggaag ctattgtggt agtgcgtatt gagggggccg gtctggcaag acagtatcat
     1561 gtgcttcagc ctggaggtct ccgtaagaca ctttctcacg atgcattaat ggccgtttat
     1621 tctggttatt gttggtttgt tcagccacag gcaaagcagg atcgtcgctc ggagctgcca
     1681 gagtatgaca tgagcggggc atggttctgg cgggtactgt ggcgattcag ggcatattac
     1741 ggacaggtta ttgttgcgag ttttatcatt aactttcttg ctctggtcag ttccctgtat
     1801 gtaatgaacg tctatgaccg ggttatacct aatcgttcct gggaaacgtt atggatgctg
     1861 agtatcgggg tgattacagc aattctgttt gagtgtgctg caaagttaat ccggagttat
     1921 ctgacggatg ttgcgggtaa gaaggcagac ctgattatca gctctgctct gctacgtcgg
     1981 gtgatggcgg taaaaatgtc tgacaggccg gcatcctctg gatcatacgc cagcaatttg
     2041 cgggaatatg agtctgtcag ggagtttatg accagcgcta cgttactggc ttttgttgat
     2101 ctgcctttta ttctgctttt catcagtgtt atcgggctcg tgggaggctg gcttgcggtt
     2161 gtacctctta cgctggtccc gctggttatt ctggcaggaa tattactgca gcgaccgctt
     2221 tcccgtcata ttaatgcctc catgcgggaa tcatcgcagc gtcagggatt acttgtggag
     2281 gctctggacg gaatagaaac cctgaagctg aataatgctg ttaactgggt tcagcgacgc
     2341 tgggatggat atactgcaag tgtcgcccgt tcgtccattc gggtaaaaaa taccagtaat
     2401 gtcataatca gcttttccac agcaattcag caaattaata ctgttctgct ggtcattctg
     2461 ggaacgtacc ttattcacag tgatgaccca cataaccgta tcaccatggg ggcactgatt
     2521 gcagccgtta ttctttccgg ccgcgcgctt gcaccgcttg gccagatagc atcactggcc
     2581 acccgtttcc agcaggcccg tctggctatg cagggggtga atgaaatagt tcgccgcccg
     2641 gtggaacggg aaagtcagac acattatctg accgtgaagg atatccaggg ggaattccat
     2701 ttcagcgcag tcacgttttt ctataagcct gaattacctg cagccttgaa aggtatgtct
     2761 gtctgtatcc ggcccggaga aaaggtgggc attattggca ctatcggatg tggtaaatcg
     2821 acgctactga aaatgatggt agggctatac cgtcaaaata aaggacaaat tacccttgac
     2881 ggggttgcta tgcagcaact ggatccctgt tttgttcggg ataaggtttt cctgcttgag
     2941 cagaatcccc ggctttttct gggaactctg cgtgaaaatc tgcagctttc gcgtcttccg
     3001 ggaagcctgt cggatgtgga tatgattaat gcagccggac gtttcgggtt tgaccgtttt
     3061 attcgtcatc acccatgcgg gcttgatatg cagatagggg aaggaggtca ggggctgtca
     3121 ggaggacagc gtcagatggt cgctctgacg agaatggtac tgggttctcc ccgggtagtt
     3181 ctgcttgatg aacccacgac gggtcttgat ctgcagacag agaacattgt tctgaaaggg
     3241 ctgaatcact ggagccgtgg acgcacaatg gtggtggtga cgcatcgtct gcaggtgctg
     3301 aatattgttg accgtattat cgtgatgaat gaaggccggg taatgatgga tggtccccgc
     3361 gatgaaatac tgaaaaaact gtcagggaaa cagcagagtg gggagagcag agcgataact
     3421 tcatgagcgt caagcggata agaaataaag agcatatatg tttaacagtt tgtggaccgc
     3481 aggggtatga cagaaaaaac ggaacggaat aaagcgtcgt cagaggtggt taggcagtca
     3541 gacctggccc tgttgacaga tgtacaggca gcccttcagc aggaaaagca tcatgccctt
     3601 ttcctgatgg tgatttttct gctggtactg gtagtgacgt ttgttatctg ggcatggaac
     3661 agtcctctgg atgaagtgac ccgtggtcag gggagtatta ttcccggcag cagggagcag
     3721 gttatacaga cccttgaccc gggtattttg aaaacacttg aggtcaggga aggagatat
//
LOCUS       DAZFRN010000280         3751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFRN010000280 DAZFRN010000000
VERSION     DAZFRN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.283"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            35..1093
                     /locus_tag="ACHKUB_005002"
     CDS             35..1093
                     /locus_tag="ACHKUB_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6657884.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHQRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTAKAAMALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            1134..1209
                     /locus_tag="ACHKUB_005003"
     tRNA            1134..1209
                     /locus_tag="ACHKUB_005003"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1167..1169,aa:Ile,seq:cat)
     gene            1219..1295
                     /locus_tag="ACHKUB_005004"
     tRNA            1219..1295
                     /locus_tag="ACHKUB_005004"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1253..1255,aa:Arg,seq:tcg)
     gene            1309..1385
                     /locus_tag="ACHKUB_005005"
     tRNA            1309..1385
                     /locus_tag="ACHKUB_005005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1343..1345,aa:Arg,seq:tct)
     gene            complement(1601..1680)
                     /locus_tag="ACHKUB_005006"
                     /pseudo
     CDS             complement(1601..1680)
                     /locus_tag="ACHKUB_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2220..>3751
                     /locus_tag="ACHKUB_005007"
     CDS             2220..>3751
                     /locus_tag="ACHKUB_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6657885.1"
                     /translation="MAFKHYDVVRAASPSDLADTLAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGILLVPCCRGASAFTTGADGTYSESAGASENSLRWG
                     VGKPLYQDLVSRTKAALAKNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFRT
                     ELAGQASQSTGGSASAVPWICGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLMT
                     DENGANVQTNNPAEDPDLEAVGYYGSKWRNDQKTWTSVDRASHFSSWARRGIISDRLA
                     TAILVHAGRTAEFITGKQPDTVKPTGPSGEGPEREPEAPVSNRTLMSLLASGEDLASQ
                     GWRYYHKPASGDSVNKNIAEAVVSDEGATGGKALQLNKPESHIWFLEHDAAGQGAELL
                     KKG"
BASE COUNT          905 a          892 c         1123 g          831 t
ORIGIN      
        1 agaatgagcc tatcagagac aggcttaatg atccatgctt aacactgtaa aaatatccag
       61 ttgtgagtta atcaacgccg actgcctgga atttatccgg tcgttacccg aaaattctgt
      121 tgacctgata gtcacggatc cgccgtactt taaagtgaag cctgagggct gggataacca
      181 gtggaagggc gacgatgatt acctgaagtg gctggaccag tgtcttgcgc agttctggcg
      241 ggtgctgaaa cctgccggaa gtctttacct gttctgtggc catcgcctgg catctgacat
      301 tgaaatcatg atgcgtgaac gcttcagtgt gctgaaccat attatctggg cgaagccgtc
      361 cggacgctgg aacgggtgca acaaggaaag cctgagggcg tatttccccg ccacagagcg
      421 cattctgttc gcggaacatt atcaggggcc gtatcgtccg aaagatgccg ggtatgaggc
      481 gaagggcagg gcactgaaac agcatgtgat ggctccgctg attgcttact ttcgtgatgc
      541 gcgtgctgcc ctggggataa cggcaaaaca gatagtggat gccacaggaa agaaaaacat
      601 ggtgtcgcac tggttcagtg ccagtcagtg gcaactgccg aacgaaagtg attatctgaa
      661 attacaggcg ctgtttgccc gggtggcaga agagaagcat cagcggggtg aactggagaa
      721 gccccaccac cagctgctgg agacgtatac ttcactgaac cggcagtatg cggaactgca
      781 gagtgaatat aagcatcagc ggcggtattt tggcgtgacg gcgcaggtgc cgtacaccga
      841 tgtgtggacg cataaaccgg tgcagtacta tcccgggaaa catccgtgcg aaaaaccggc
      901 agaaatgctg cagcagataa tcagcgcgag cagtcgtccg ggggacctgg ttgcagattt
      961 tttcatgggg tcgggttcga cagccaaagc cgcgatggca ctggggcgtc gtgcaactgg
     1021 tgttgagctg gagactgaac gttttgagca gacggtcagg gaagttcagg atttagccag
     1081 tcagaacgga tgatattgca ggattagtta cgtaccgtta ttatcctgca ctcggccctt
     1141 tagctcagtg gtgagagcga gcgactcata atcgccaggt cgctggttca aatccagcaa
     1201 gggccaccat atcacatacc gccattagct catcgggata gagcgtcagc cttcgaagct
     1261 ggttgcgcgg ggttcgagtc ctcgatggcg gtccattatc ggtattcagc gttgttagct
     1321 cagccggaca gagcaattgc cttctaagca atcggtcact ggttcgaatc cagtacaacg
     1381 cgccacactt attttttctg tctcactttg gtggacctgt ttgttgctaa aaaaggaagc
     1441 atcaggtggt tagccgggta tcagtcatgc cccgaaaatt ttaaatgtct cacaattcag
     1501 tcagttgaca gttgcctgtc agactgagta tttgttaaaa gaaactggca tggtgaatcc
     1561 ccctgtgcgg aggggcatat cagcgacagg tggttggtta tatcccttat ctggtgcggg
     1621 ttcaggtgct gatactgaac tcaccgggag gcacccggca ccatgcgcat gatgatacag
     1681 atacgcggct ttagcccctc tccggagggg ctttttaatg aggggaaggc accgggcagt
     1741 gaaatgttaa atcactcaca attcagtcag ttgacggttg cctgtctggc tgagcatttg
     1801 ttaaaaaaat cctgcatgat gaatccccct gagcggaggg gcataatgac agatgtttgg
     1861 ttgcgtattg tataggcaag ttgcggattc tgtctggtca ttgcagaatt caccgggagg
     1921 cacccggcat catgctgtat acagagatta ggcatatatc caggctcctc atcgcaggag
     1981 cctttttaca tgcaaaaaaa gcccgcgccg ggagacgcgg gcagcaagga ataaacaaca
     2041 aaacgtgaag taatcaattt ttcagctggc gaataatacc cgacagtaat cactctgcgc
     2101 aactgcgcgg cctttttcgt attgcgggct gtagtcttcc ttctgtcatt gtcctgtaac
     2161 ttccggactt cagcccgccc cttatctgac tcacaacatt atcccggccg ggaggattca
     2221 tggcatttaa acactatgac gtggtcaggg cggcgtcgcc gtcagacctt gctgatacac
     2281 ttgctcaaaa aattcgtgaa ggatggcagc catacggcgg gccgttttct tcgtatacgg
     2341 atgatggcgc agcacttatt caggcgattg tcgcagaagg tgatgtgagc acacctgtgg
     2401 tggtgaagcc gtcggatgga gaaggcacag taatcagcgc caccagcgac ccggagtatt
     2461 actttgttgt ggttctggca gggcagtcaa acggcatgtc gtatggtgaa ggccttccgc
     2521 tgccggagac atatgaccgt ccggacccgc gtattaagca gctggcgcgc cgcagtacgg
     2581 tgacaccggg cggtgccgcc tgtaagtata acgacattat tccggcggac cattgtctgc
     2641 atgatgtgca ggacatgagc cgtcttaacc atccgaaagc ggacctgtca aaggggcagt
     2701 acggaaccgt ggggcagggg ctgcatatcg ccaaaaaact gctgccgttt ataccggcga
     2761 atgcgggcat tctgctggtt ccgtgctgcc gtggtgcttc ggcattcaca acgggtgcag
     2821 acggcacata cagcgaatca gccggtgcat cggaaaattc actgcgctgg ggtgtgggta
     2881 agccgctgta tcaggatttg gtgagccgga ctaaagccgc actggcgaag aacccgaaaa
     2941 accgtctgct tgcggtggtg tggatgcagg gagaaggtga tgcggcagtg gggacgcatg
     3001 cgcagcatcc ggggctgttt agtgcgatgg tgaatcagtt cagaacggag cttgccggtc
     3061 aggcatcaca gagcacagga ggcagcgcat cagcggtacc gtggatttgc ggggacacaa
     3121 cgtatttctg gaagcagcgt catgcggaag ggtatgcatc agtatacgga gggtataagg
     3181 ggaaggagtc gcagaacatt ttctttgtac cgttaatgac ggatgagaat ggtgcgaatg
     3241 tgcagaccaa taacccggca gaagacccgg acctggaagc cgtcggttat tacggttcaa
     3301 agtggcgtaa tgaccagaag acctggacat ctgtggacag ggccagccat ttcagttcat
     3361 gggctcgccg tgggattatt tccgaccgtc tggcaacggc gattctggtg catgccggga
     3421 gaaccgctga attcattacc ggaaaacagc ctgatacggt gaagcccacc ggaccttccg
     3481 gtgaaggccc ggagagagag ccggaagccc cggtcagtaa ccgaaccctg atgagtctgc
     3541 tggcgtccgg tgaagacctg gcatcacagg gctggcgcta ttatcacaaa ccggcgagcg
     3601 gagacagtgt taataaaaac atcgctgagg ctgtggtcag tgatgagggt gccaccggtg
     3661 gtaaggccct gcaactgaac aaaccggaaa gccacatctg gtttctggag catgatgccg
     3721 ccgggcaggg agcagagttg ctgaagaaag g
//
LOCUS       DAZFRN010000281         3486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFRN010000281 DAZFRN010000000
VERSION     DAZFRN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.284"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            35..1102
                     /locus_tag="ACHKUB_005008"
     CDS             35..1102
                     /locus_tag="ACHKUB_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6657886.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELDT
                     GRFEQTAQEIRDVFSGSGLQK"
     gene            1166..1891
                     /locus_tag="ACHKUB_005009"
     CDS             1166..1891
                     /locus_tag="ACHKUB_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6657887.1"
                     /translation="MDVACSVVLIRYPIDIFLEKEKVSLDAGSVMLVARNIRGLFCAY
                     ADRVKMADISNSVVNQYLERECELTEYTPTKMPLYLMSDIYNTELAEALITQHSESSD
                     TLKDFSVITAFSCISLFATDRRLPLFLSGAVNNISCKVRAIIQTDISAGWMLGVIALQ
                     LHMSESLLKRKLKDEGYSFSRLLLEERMRVAVNLVCFQRECGQAIAEKCGYSSKSYFI
                     SVFHQYYGAPPESYAYLQKADVI"
     gene            3224..>3486
                     /locus_tag="ACHKUB_005010"
     CDS             3224..>3486
                     /locus_tag="ACHKUB_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO6657888.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQS"
BASE COUNT          889 a          703 c          957 g          937 t
ORIGIN      
        1 agaatgagcc tatcagagac aggcttaatg atccatgctt aacactgtaa aaatatccag
       61 ctgtgagtta atcaacgccg actgcctgga atttatccgg tcgttacccg aaaattctgt
      121 tgacctgata gtcacggacc cgccgtactt taaagtgaag cctgagggct gggataacca
      181 gtggaagggc gacgatgatt acctgaagtg gctggaccag tgtcttgcgc agttctggcg
      241 ggtgctgaaa cctgccggaa gtctttacct gttctgtggc catcgcctgg catctgacat
      301 tgaaatcatg atgcgtgaac gcttcagtgt gctgaaccat attatctggg cgaagccgtc
      361 cggacgctgg aacgggtgca acaaggaaag cctgcgggcg tatttccccg ccacagagcg
      421 cattctgttc gcggaacatt atcaggggcc gtatcgtccg aaagatgccg ggtatgaggc
      481 gaagggcagg gcactgaaac agcatgtgat ggcaccgctg attgcttact ttcgtgatgc
      541 gcgcgctgcc ctggggataa cggcaaaaca gattgtggat gccacaggaa agaaaaacat
      601 ggtgtcgcac tggttcagtg ccagtcagtg gcaactgccg gacgaaagtg attatctgaa
      661 attacaggcg ctgtttgccc gggtggcaga agagaagcat cagcggggtg aactggaaaa
      721 gccccaccac cagctgctgg agacgtatac ttcactgaac cggcagtatg cggaactgca
      781 gagtgaatat aagcatctgc ggcggtattt tggcgtgacg gcgcaggtgc cgtacacgga
      841 tgtgtggacg cataaaccgg tgcagtacta tcccgggaaa catccgtgcg aaaaaccggc
      901 agaaatgctg cagcagataa tcagcgcgag cagtcgtccg ggtgacctgg ttgcagattt
      961 tttcatgggg tcgggttcga cagtcaaagc cgcgatggcg ctggggcgtc gtgcaactgg
     1021 cgttgagctg gataccgggc gttttgaaca gactgcgcag gaaatcaggg atgtatttag
     1081 cgggagcggt ttgcagaagt aatctcccaa aatgccattt ttggaatata ctggtattaa
     1141 gtctttttgc tcaggttaat tcaaaatgga tgttgcctgc tctgtggtcc tgattcgcta
     1201 tccgatagat atttttcttg aaaaagaaaa agtttcactc gatgccggaa gtgttatgct
     1261 tgttgccaga aatataagag ggcttttctg tgcttatgcc gacagagtga aaatggcaga
     1321 tataagtaac agcgtcgtca atcagtatct ggaaagggag tgtgaactga cggagtatac
     1381 accgacaaaa atgcctcttt atttgatgtc tgatatttac aatacggaac tggcggaggc
     1441 tttaataacc cagcatagtg aatccagtga tactttgaag gatttctctg ttataaccgc
     1501 attttcatgt atctccttat ttgcaacaga caggcgtctg ccattatttt tgtccggtgc
     1561 tgtaaataat atcagctgta aagtaagggc tataattcag accgacatat cggcaggctg
     1621 gatgctaggt gtgattgcat tacaattgca tatgagcgaa agcttgttga agcgaaaact
     1681 taaggatgaa ggttatagct ttagtcggtt gttgctggag gagcgaatgc gtgttgctgt
     1741 taatcttgta tgtttccagc gagagtgtgg acaagcgata gcggaaaaat gtggttactc
     1801 aagtaagtcc tatttcattt ctgtatttca ccagtattat ggtgcaccac ctgagagcta
     1861 tgcatatttg cagaaagcag acgttatctg atgttttttc atcaggcata tattgattga
     1921 ctgtattaga tatagtctga atgtgttcag cataaagagt tataatcatt tggtgtttgc
     1981 gtgaatattg atgagaaaat cagatgtagc caactgttac agcgtattga agccgtcaat
     2041 gtggaacgaa caaaggtctt cagtcgtctg acagttttat tttgtacccc tgatcgacgt
     2101 tcaggtcagg agatggttct tctggatgta gatgcacttc ggaacgtctg tgaggagttc
     2161 accgcggcta attcggagtt actgagtctt gttcaggagt acaacagaat tgcaggtagt
     2221 aacggttttg atgaaatcaa aattatatcc cgtggataaa acatatcctg tatatttatg
     2281 ttgttacata caggcgcttt tagtatctgc cgcacggcag ttgtacttca gaataagatg
     2341 cttttgtgac tggtggtgtt gtatctgggg ttagccagga ggcaggttca gagagtccgg
     2401 tggtaattat gtgtatgcag tggttgttgc tgtaccgtgg gcaagttctg gaactggctt
     2461 cgtccattgt agatgattgt tagctggata tcagttacct catggaaatg ttaaatgact
     2521 cacaattcgg ttgatggact attgtctgat cgatggaagt cttgtaaaaa cgagccagca
     2581 tagtgaatcc ccctgtgcgg aggggcgtct gacatggtct ccatagcgat ggtcataata
     2641 cgcagattca gtagtcaggc tgaatctacc gggaggcacc cggcactatg ctgcttatgg
     2701 tgtatacata gcgatcggcc cctctccgga ggggcttttt tatgcaggga aaaggctgtg
     2761 ttgtgaaatg ttaaattcct cacaattcat gaggttgatg attgtctgat tgatgaggag
     2821 tttgttaaaa aaaacgggca tggtgaatcc ccctgtgcgg aggggtgata tcgtatcatt
     2881 tcctgtgtgg ccgactgata tcacggacat ttgttcaccg ggaggcaccc ggcaccatgc
     2941 agtatacaga gattaggcat acatccaggc tcctcatcgc aggagccttt ttacatgtaa
     3001 aaaaaccgct cctgggaaga gcggttggca aggaagcaag gaataaataa caaaacgtga
     3061 agtaatttat tcagcaccct aataatacct tagagtaatc accttgcgca actgtaaggg
     3121 cgtatttctt ttttgcgggc tgtttttctg tgtgactttt gtgtttccgg aggtcagccc
     3181 gtgccttctc tgactcagaa cattatcccg gccgggagga ttcatggcat ttaaacacta
     3241 cgatgtggtc agggcggcat cgccgtcaga ccttgctgaa cgactgacac aaaaactgaa
     3301 ggagggctgg cagccatttg gcagtccggt ggccatcacg ccttataccc tgatgcaggc
     3361 cattgcggcg gaaggtgatg tcaccacacc agtggcggtg accggtaatg agggtaaggc
     3421 ggtggctgtc agtgccacca gcgacccgga gtattacttt gttgtggttc tggcagggca
     3481 gtcaaa
//
LOCUS       DAZFRN010000282         3462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFRN010000282 DAZFRN010000000
VERSION     DAZFRN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.285"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            524..732
                     /locus_tag="ACHKUB_005011"
                     /pseudo
     CDS             524..732
                     /locus_tag="ACHKUB_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            719..908
                     /gene="tnpB"
                     /locus_tag="ACHKUB_005012"
                     /pseudo
     CDS             719..908
                     /gene="tnpB"
                     /locus_tag="ACHKUB_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(911..1099)
                     /locus_tag="ACHKUB_005013"
     CDS             complement(911..1099)
                     /locus_tag="ACHKUB_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO6657889.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            1634..1775
                     /locus_tag="ACHKUB_005014"
                     /pseudo
     CDS             1634..1775
                     /locus_tag="ACHKUB_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            1998..2735
                     /locus_tag="ACHKUB_005015"
     CDS             1998..2735
                     /locus_tag="ACHKUB_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6657890.1"
                     /translation="MIVCYVILVNRPFETEIAGKKIFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGFIESRIAIK"
BASE COUNT          952 a          772 c          704 g         1034 t
ORIGIN      
        1 ttctttctca agtgttattt cttgaccttt atcacagaac catctccctg gatatcccca
       61 tcacattgca tgaaaagcaa gttttaccaa tcttgatttt cagtgttaaa taagattaat
      121 aactgtaatt ctgcaaaaaa aagaacacca tatgtagtat gactacgtat tccactctgt
      181 tcgttcacgg actggcatcg aagtctgagg tgtactggca atagcggata cagtcatttg
      241 ttcttttttt aagcattcat ccgatgatac ttttcctgaa tgctaccgga gtggtattcc
      301 cctgccgtga gggagggcgc tgacggttat aaaaatcctg atatataccc attcccgtat
      361 gacagcgatg gcgtcacatc tctgttctac catcttcaca gaaatatttc gttcattagc
      421 attccggtat tcgtataacc accttcggtg gagaaagaat aaacaatccc tggaatgcga
      481 tgagcgcttg accagaaaag catcatattg cttcatggcg catcagcgac cacaggatgc
      541 cgcaaaagcc gaaaaacgga ccagagcgcc tgcaatcatt cctcgcctca gtatctgttg
      601 cccatggagt ggccgataac agatcattgt cggatgagcc ttatcccctt ccttcccggt
      661 ggtatccgga tgtgctgcca gccttctcag ttaaccgatg ttttccgggc tttttgccat
      721 actgaggact gataacgtat tcctggtcgt aaaacccgtc aatatgcgcc ggggcatcga
      781 caaaactgac acagtacgtc cggaaagaac ttaacgctgc atggcacgac aatgctgcct
      841 ttgtctttac caacaaggct cgttcccgca taaaagttct acgccgggat aaacatggcg
      901 tatggctgcg tcaggccttc actacttcac accggaagtc ttccaccctg acacgaatat
      961 accgtccggg ctctttcgca ggtgcaaaca gtttttcaca ttcggcgaat gtcatttctg
     1021 aatcctggta acgcaaccac tgaaccggca tattaccggg tttgattatt gtcgcagtta
     1081 ttttatatcg tttccccata tcgcctctca tcctgacaac aaaattaccg gctgagccat
     1141 cactccggta aagcatgcga aaaaaagtga gcgtctcagc aatggctcat gcgatgattc
     1201 tgtacgggca ccgatggcac ctcagaatac aacaaagata tcagagtaac atgtctctct
     1261 cgttattttt cagagttctt gacaccctcc ttctgccaca ttgtcaacgc cagtggagac
     1321 ttctgctgtc agttttttca tccatacaga gtgacattaa ttatcctgaa caaaacgaaa
     1381 tatcctgaat atatttattt tctcctggtg ccggagacat gatgatgtac aggctcaggt
     1441 gcggtgtgaa tgtaaaatgg tatgttaatt attcatttct gaatgcaaac tgaatgcaaa
     1501 cttattccga attgaattct cctgacacca catgataaaa atgaggatat aataaaatac
     1561 ttctcttctg gatgtgccat tacctgccgt tgctgtaata cggcgggtat tttttactga
     1621 catatcggga agccagcaga cgttcgtagc tacatccccg aaagtggcac actgcaaact
     1681 ataatacgag taaaacatcg atcaccgtag ctgatgtgtt caacgatcgt gcactagcgt
     1741 tctgcgattc tcagggactg cctgttctga gaatcggcga gtccgcgatc ggatacgatg
     1801 cgatttcgca gtgactcagg gattgcatct gaaaaccctc cggctatgtc ggtaaatatt
     1861 tatttctatg aaccgtacga ttcagatagc attcgcccgg ccccgtaaga ataatctccg
     1921 gacaaataag atttttcctg aaaaagcgtc tagtcttttg atatcgttgt ttttatgcta
     1981 atcagggagt atgtgtgatg attgtctgtt acgttattct tgttaaccgt ccgtttgaaa
     2041 cagagattgc cgggaaaaag atattttttc agaaaaatga cgtcctgttg acagataaca
     2101 tcagccagtc ctttttcctg cgccatcccg gtaatgttac tgagctccgg attgatgaat
     2161 gtattgtcag aaaatatatg gaaagtgccg acaggatcca gcactctgat agtgctgttt
     2221 ctgttccgtt cattaaaaca accttctctc acatcgctct gttacataat gtcatgcaga
     2281 atctcagtct gcaaaagaca ttgccacaac aattttctga gcagatgttt tttgcctgcc
     2341 tttccatttt ttccacacac agaggtttca tgtttctgct attcagatgc ataaactctg
     2401 ttgcgcttaa agtcaggtat attatctgtt ccagtccatc acgggcatgg agagttaaag
     2461 atatcagtaa ttctctgtac atgagcgaga gtttactgaa aagaaaactt caggaggaaa
     2521 aagtttcttt ctcccaaatc ctgctggata tacgcatgca taacgcccga caacttattt
     2581 ccagtaaact gtcagtgaat caaatcgcaa tacggtgcgg ttatgccagc acaccgtatt
     2641 ttatcagcgt tttcagagaa tattttggca taacacctca tcagatgatg attaatcagt
     2701 ccggatttat tgagagcaga atcgcaatta agtaaagtat gtcagtcagc gcataataaa
     2761 agtattctta tgcttcatta tcagtttctg tttatcactc cggagcactg tagtaactac
     2821 ggccccggaa aacagatggt aatgagttga ctgactgatg atattgtggc gacattttta
     2881 cggatgcgag caaagaatgt attggtccgt ttgattatgc agtgattaac aaaactccgg
     2941 gaagatgaat gagtatacct ctcctgcaat ccgtttcctg agaaaacagg tggtaacaca
     3001 atattcttaa tatgttactg tttcaccaca caaccaggtt tacggttgtg tggtgaacta
     3061 tagtaatccc gggtaacaat ccggagctgt tccttttata ctcttcaggc tgtgtactca
     3121 tccacgccat ccgtttttca gtctcagtgt gtgaataata aattacactt tgaactgaac
     3181 gccagtcagg accatgtcgt atttccgggt ggatgagcat atatcagaaa ctacaataac
     3241 tacagataac ggttttcagt aattctgtca taacttgccg gaatgcaatg tttttgtgac
     3301 atggttctta ttttttatac acaggcaggt tttgtcggga tggaatggat aatgaactct
     3361 tatcattata aggcggtaac ctctttttta tgtgattaac tttcattatt accacttctg
     3421 tttatcgatt cagctctgga gatacagaaa aattcagtgc ag
//
LOCUS       DAZFRN010000283         3455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFRN010000283 DAZFRN010000000
VERSION     DAZFRN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.286"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(338..2380)
                     /locus_tag="ACHKUB_005016"
     CDS             complement(338..2380)
                     /locus_tag="ACHKUB_005016"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO6657891.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLTRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGSLITAMKNASSGVT
                     DPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKPGDVGDKLN"
     gene            complement(2383..3087)
                     /locus_tag="ACHKUB_005017"
     CDS             complement(2383..3087)
                     /locus_tag="ACHKUB_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO6657892.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            3133..>3455
                     /locus_tag="ACHKUB_005018"
     CDS             3133..>3455
                     /locus_tag="ACHKUB_005018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657893.1"
                     /translation="MRITDNKKTNQAEYTFNAAYFLIAVATGNTAHFLTSVATGSTAH
                     FSISVATGSTAHFLISVATGNAAYFLITVATGNTAHFLITVATGNTAHFLITVATGST
                     AHFSIS"
BASE COUNT          820 a         1020 c          734 g          881 t
ORIGIN      
        1 aagcgacatg tagccacccg aaggcgctcc tctcaggcca cttgtggagc attggtggtc
       61 gccggagcga cgttatgtgc ccggacagac tgaaatcact gatggaaaaa gtcaacagct
      121 aaccaaaaac aaaaaagcac cggcaagccg gtgcttcagg gggataccaa atcccggtgt
      181 tgattctcat cttctccgga gagagcgact cccggcacgg ttttcacgga gtgcatgtgc
      241 cttgtaaccc cgccgtgtgc ataacgggtt accggacctc tcagggctgt cagccccact
      301 gtggtcaaca gggtgatacc agaactcgcc cgcaatttta attcagtttg tctccgacat
      361 caccaggttt ttgtttatga gaaaaattaa tttttctttt cctgcccctg ctgtttccct
      421 gctcaccagc aattcccctg caatcgggat aacgggagca tccccagaaa aaaccgcttt
      481 ttcccttccg tttcaccgtc ggaccgttac agagtggaca tgaaggacct gattccactg
      541 acagattaac cgtctggtta cgtccggata acaccaggtg cctgatccac tgatgctgtt
      601 tctccataaa cgcctccagt gagattttcc cccgggcaac atcatccagc gcctgttccc
      661 ataaagccgt catacccgga tttgtcaggg agtccggcaa tgccgccacc agttctcttc
      721 ccagttgcgt ggaacggata tgttttcccg ctctctcaat atagttacgc gcaaataacg
      781 tttccaggat ccccgcgcgt gtggcttccg ttcccagtcc cgcattctcc ctcaggatct
      841 ttttcagcgc cggatccgtt acaccagacg aagcattctt catcgcagta ataagactgc
      901 cttcagtaaa aggacgggga ggtacggttt tcagctcctt ctgttcagca ccatccagag
      961 cacagagttc tccctttatc aaagggggca atatgtttcc actttcatca ctgtcaggct
     1021 tttcttcatc tgcatgattt tccggtggaa ataactgttt ccacccgata atcacattca
     1081 ctcttccgcg ggaacggaat aactgccccc caatcacaaa tgttgcctca gtaacatcgg
     1141 cttcctgctc cggcagaaac tgtgccagat agtgctgacg aatcagttga tataccttca
     1201 gttcatcacc gttcagtgag ctgaggtcaa aaacctgccg ggtcggaata atggcatggt
     1261 gagccgtaat ttttttatca ttccagatac gggatacccg tcctgattcc agttcagcca
     1321 gttgtggaag cacggacgga tctgaacgcc cgacagcctc aagcacttca gaactctcct
     1381 catgcatcga ggcaggcaga taaccacagt ctgtcctggg ataagttgtt gctttgtgag
     1441 tctcataaag ggactgtgcg atatccagaa cggtctgagc ccccatgcca aacttccggg
     1501 aacaggcttc ctgcaacgtt cccagcgaaa aacacaaagg tggcggtgtt ttctccctct
     1561 tctgtacaac atcctgtaca acagcagagg actgctgacg gcacagaccg agaacagcac
     1621 cggccgcctg cacattcaca caacgtccct catcatcgca gtactgttca gctgccaccc
     1681 agttactgcg gaaacggagc ccctcctttt ccagccagac aatcacctgc caccaggaaa
     1741 ccggcacaaa tgtggcgatc tcctgatccc gtctgaccac cattgccagc gtgggtgtct
     1801 gcacccggcc cactgacaaa accccagaaa ttccacgttc ccgtgccttc agggtataca
     1861 accgggttag attcatccca gtcagccagt cagcccgggc acgcccctta ccggcttcac
     1921 tgagaagcgc agtctgttcg cccgggagaa tattattcag cgccttcctg atactggcct
     1981 catccagtgc agataaccac agacgcctga cacgccctct gtactgacag tactccagca
     2041 actctctggc tatcacttct ccttcacgat cggcatctgt ggcaataacc acttcagaag
     2101 cgcgttttaa cagcccccgg atcacaataa gttgtttttt caccttcggc ttcaccacca
     2161 tctgccattg ttccggtatc agcggcaaca catcagaacg ccatggggaa ccgaactgtt
     2221 caccgtaagc ttccggctca gccagttcca gcaggtgtcc tatcgcccag gtgactgcaa
     2281 tattgtcatc tccgatgtat ccgtcgcccc gcttactgag cgccagttca cgggcgatat
     2341 ctttcgcctg agaaggtttt tcacacagaa agagttgcat actcaccccg ctttaaccgg
     2401 tggtgaataa acagaacgtt tcttaccgct catcacgtca ggatccggct cccccatgcg
     2461 gttaaccgcc tcctttccac gggctgtacc ggacaaaata tcagcacgtg taacactgac
     2521 tgatttataa ttttgaataa tcccgtaaat cctgcgtacc gcataccctc cctgattcag
     2581 caactcatcc cgttttgtgc gggaaataag tccgtaatga aatacctgaa ataccttcat
     2641 gaccagtcga tcatatccga tcagaagcca tacacatcga taaccgaccg gagaactact
     2701 gtacacgcca atatttaccg gcgaagagga tttaatatca gaaagggaga tctgtcccgg
     2761 caggttttta aggatcccgt caagccacgc aagatgcttt tcaattgttt cagttgcctt
     2821 cgtcagggca ttttccagcc tgactaacat catatctgca taaggattat ccctggctga
     2881 atctctggtt gcctgacccg caaacgcaat agccctgggc atactgatga tgtcaggccg
     2941 cttttcctgt ttttcctcct tctgattatt cctgcgtccc tcccagagac ggatcgcata
     3001 tcgggtatga agggcaatag tcagcgatga ctgcagagca ccaacttgtt ctgattttct
     3061 gacttctccg gttttctcct ggggcattgt gtttactcca aatgatttca tgaccagaag
     3121 atagtccgca ggttgcgtat taccgacaac aaaaaaacaa atcaggcaga atatacattt
     3181 aacgccgctt attttttaat cgctgttgcc actggcaaca ccgctcactt tttaacctct
     3241 gttgccactg gcagcactgc tcatttttca atctctgttg ccactggcag caccgctcat
     3301 tttttaatct ctgttgccac tggcaacgcc gcttattttt taatcactgt tgccactggc
     3361 aacaccgctc actttttaat cactgttgcc actggcaaca ccgctcactt tttaatcact
     3421 gttgccactg gcagcactgc tcatttttca atctc
//
LOCUS       DAZFRN010000284         3389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFRN010000284 DAZFRN010000000
VERSION     DAZFRN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.287"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..560
                     /gene="lpfB-O113"
                     /locus_tag="ACHKUB_005019"
     CDS             <1..560
                     /gene="lpfB-O113"
                     /locus_tag="ACHKUB_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO6657894.1"
                     /translation="VFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            585..3107
                     /gene="lpfC-O113"
                     /locus_tag="ACHKUB_005020"
     CDS             585..3107
                     /gene="lpfC-O113"
                     /locus_tag="ACHKUB_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO6657895.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3118..>3389
                     /gene="lpfD-O113"
                     /locus_tag="ACHKUB_005021"
     CDS             3118..>3389
                     /gene="lpfD-O113"
                     /locus_tag="ACHKUB_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO6657896.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSK"
BASE COUNT          932 a          740 c          823 g          894 t
ORIGIN      
        1 ctgtatttag caaagaagaa aaaatacctt atctcatcca ggcttgggta gatccattta
       61 ataaagagga taaaagcaaa gcgccgttta ccgttattcc gccagtatca cgattagaac
      121 cttcgcaaga aaaaatatta cgcattattc acaccaaagg tgtgtcattg cctgatgatc
      181 gcgagtcagt cttctggctg aatattaaaa atattccgcc atcagcgagc aacaaagcga
      241 cgaactcgct ggaaatagca gtaaaaacgc gtatcaagct gttctggcga ccagcaaata
      301 tccgccttat tcccgaagat gctgcgccga aagtgaaatg gcgccgggaa gggcgaaatc
      361 tgatagcaga gaatcccaac ccaattcata tcagtgtgat ggatgtcatc gttgatggcc
      421 atgatgtgcc cctcaatatg atccgtcctt ttgaaacgct cacgttacct ttacctgcta
      481 attctgcggg aggccaaatg acctggcgat ttattaatga ttacggcgca gtcagtgacc
      541 cgataaaaat gacgctgtaa gcaagggata aggataggcg tattatgatg acgaccagaa
      601 tagtggttgg cctcacggca gggacgtgtc tgattttctc gcaaaacctg atggccgagg
      661 tcagtgtatt caatccggcg cttctggaaa tcgaccatca atccggagtc gatattcgcc
      721 agtttaatcg ggcaaacctg atgcccccag gtgtttatag cgttgatatt tttatcaacg
      781 gtaaaatgtt tgaacgtcag gatgtgacat ttgttcagga taatccagat gctgatctgc
      841 acgcttgctt tattgccatt aaaaaaacac tgtcctcctt tggcataaaa gttgatgcgc
      901 tcaaatcgtt caatgatgtg gatgagacgg tttgcctcga tcctgctcca cgtattgaag
      961 gctcatcctg gcagtttgac agtgataaat tgcagctgaa tatatccatt ccccaaatct
     1021 acatggacgc gatggcttat gattacatca gccccacgcg ttgggatgag gggattaacg
     1081 cgctcaccat caactacgat ttttctggtt cacatacact acgttcagat tatggttcac
     1141 aagagacaga taccagttat ctcaatctgc gcaatggact gaatattgga ccgtggcggc
     1201 tacgtaatta cagtacttta aacaccagcg atggccgtgc ggaatacaac tccattagta
     1261 cctggataca gcgcgatatt gccgcgttaa gaagccagat tatgattggt gatacgtgga
     1321 cggcgagcga tattttcgac agtacgcaaa ttcgcggcgc gcgtttgtat actgataacg
     1381 atatgctacc cgccagccag aatggctttg ctcctgtggt tcgtgggatt gcaaagtcca
     1441 acgccaccgt catcattcgg cagaatggct acgtgattta tcagtctgcc gttccacaag
     1501 gtgcttttga gatcaccgat ctcaacaccg ccagtacagg tggcgatttg gacgtaacca
     1561 tcaaagaaga agacggtagc gaacaacgat tcacccaacc ttatgcttca ttggcgattc
     1621 ttaaacgtga aggtcagaca gatgttgatg ttagcgtggg tgaattgcgc gatgaagacg
     1681 gatttacacc ggacgtcctt caggcgcaaa tacttcatgg tttttcccac gggatcactt
     1741 tatatggagg tatgcaggct gctgaaaatt atggttctgc agctctgggt gtcggtaaag
     1801 atcttggcgc tttgggcgca atttctttcg atgtgacaca tgctcgtgcg aattttagcc
     1861 atgatgatac agaaacgggt cagtcatatc gctttctcta ttcaaaacga tttgacgaca
     1921 cagacactag cttgcgcctg gttggctatc gttactccac cgagggctac tataccctca
     1981 atgagtgggc atcgcggcgc aacagccctg aagacttttg ggaaacaggt aaccgacgta
     2041 gtcgcgtgga gggaacgcta acgcagtcgt tggggagaga ttatggcaat ttatacctga
     2101 cattaagccg gcaacaatac tggcataccg atgatgtcga acgattaatg caatttggct
     2161 acagcagtag ctggaagcgt ctctcgtgga acgtctcctg gagttattcc aatactgcca
     2221 gacaggggac ggggaacaac catgccagtg ataacaccag tgagcagatc tacatgctct
     2281 ctttatctgt tcctttatcg ggctggtggg gtaatagtta cgccacctat tctgtttcgc
     2341 aaaacgataa ttccggtagc tcacatcaac tcggactcag cggtacggcg ctggaaagaa
     2401 ataacctttc atggaattta atgcagtcct ataacagtca tgatgatgag gttggcggta
     2461 atatgtccct gacctatgat ggctcttatg gcacggtgaa cggcagctat aactacagcc
     2521 aaaattccca gaggctgaat tatggtatca gagggggaat tctggcacac agcgaagggg
     2581 taacgttaag tcaggagtta ggtgaaacta ttgctcttgt taaagcacct ggggccgccg
     2641 ggttagaaat agataatatg cgcggtgctg cgacggactg gcgcggctat acggtcaaga
     2701 cacagctaaa cccttatgat gaaaatcggg tagcaatcag cgataactat ttctcgaagt
     2761 cgaatataga acttgataat accgtcgtta cgatggttcc cacgcgtggt gcagtggtta
     2821 aagcggagtt tgtgactcat gtgggttatc gcgttctctt cagagtgtta aatgcaaatg
     2881 gtaaaccggt accttttgga gccattgctg cgatacaaga tgcaagtttg gcagattcag
     2941 gaattgtcgg tgaccgtggc gaactttatc tttctggtct accagaaaaa ggacaggtta
     3001 cgttatcctg gggagaaaac gcctcaacaa aatgcatctt caattattca ctttcgacac
     3061 cagaaagtga gagcggatta attgaacagg gtgtgacatg tcattaaaag gatttttatg
     3121 aacaagtata tcaaacagtg gtgctttgct gtgtttatgc tctcgttaag tagtgtagca
     3181 cttgcagctc ctaaaggtat ctgtacccct gataatggcg tatttcacag tacgcttgat
     3241 ttctcgggat atcttattac cgcgaacgaa aataaggttg gaacgacatt taacaccact
     3301 gtaactaatg gatcctcata tcctggacgc tgtcattgtg atactggtaa cgtaggagaa
     3361 ttcccctata tctattatac gtcaaaaat
//
LOCUS       DAZFRN010000285         3350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFRN010000285 DAZFRN010000000
VERSION     DAZFRN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.288"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            78..1229
                     /gene="yiaY"
                     /locus_tag="ACHKUB_005022"
     CDS             78..1229
                     /gene="yiaY"
                     /locus_tag="ACHKUB_005022"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO6657897.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1394..2932
                     /gene="aldB"
                     /locus_tag="ACHKUB_005023"
     CDS             1394..2932
                     /gene="aldB"
                     /locus_tag="ACHKUB_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO6657898.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3151..>3350)
                     /locus_tag="ACHKUB_005024"
     CDS             complement(3151..>3350)
                     /locus_tag="ACHKUB_005024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657899.1"
                     /translation="FQQDEVYHIEIVELLAKLMNNSSKTSTVQIKRIKPSIIYRLLLI
                     GLGSPMVIYGLVRPLTIETRD"
BASE COUNT          822 a          826 c          900 g          802 t
ORIGIN      
        1 taataataat gatcatgttt tcgaatgaaa atcctcagta agctgcccac ccttttttac
       61 actttcagga gtgtgttatg gcatcttcaa ctttctttat tccttctgtg aatgtcatcg
      121 gcgctgattc attgactgat gcaatgaata tgatggcaga ttatggattt acccgtacct
      181 taattgtcac tgacagtatg ttaacgaaat taggtatggc gggcgatgtg caaaaagcac
      241 tggaagaacg caatattttt agcgttattt atgatggcac ccaacctaac cccaccacgg
      301 aaaacgtcgc cgcaggtttg aaattactta aagagaataa ttgcgatagc gtgatctcct
      361 taggcggtgg ttctccacac gactgcgcaa aaggtattgc gctggtggca gccaatggcg
      421 gcgatattcg cgattacgaa ggtgttgacc gctctgcaaa accgcagctg ccgatgatcg
      481 ccatcaatac cacggcgggt acggcctctg aaatgacccg tttctgcatc atcactgacg
      541 aagcgcgtca tatcaaaatg gcgattgttg ataaacatgt cactccgctg ctttctgtca
      601 atgactcctc tctgatgatt ggtatgccga agtcactgac cgccgcaacg ggtatggatg
      661 ccttaacgca cgctatcgaa gcgtatgttt ctattgccgc cacgccgatc actgacgctt
      721 gtgcactgaa agccgtgacc atgattgccg aaaacctgcc gttagccgtt gaagatggca
      781 gtaatgcgaa agcgcgtgaa gcaatggctt atgcccagtt cctcgccggt atggcgttca
      841 ataatgcttc tctgggttat gttcatgcga tggcgcacca gctgggcggt ttctacaacc
      901 tgccacacgg tgtatgtaac gccgttttgc tgccgcatgt tcaggtattc aacagcaaag
      961 tcgccgccgc acgtctgcgt gactgtgccg ctgcaatggg cgtgaacgtg acaggtaaaa
     1021 acgatgcgga aggtgctgaa gcctgcatta acgccatccg tgaactggcg aagaaagtgg
     1081 atatcccggc aggcctacgc gacctgaacg tgaaagaaga agatttcgcg gttctggcga
     1141 ctaatgccct gaaagatgcc tgtggtttta ctaacccgat ccaggcaact cacgaagaaa
     1201 ttgtggcgat ttatcgcgca gcgatgtaat catcatttcc acaacggctg gcaaattgtc
     1261 agccgctttt tcaactatct ctgtaaccct tgcccgtaaa ttcgtgatag ctgtcgtaaa
     1321 gctgttaccg actggcgaag atttcgccag tcacgtctac ccttgttata cctcacaccg
     1381 caaggagacg atcatgacca ataatccccc ttcagcacag attaagcccg gcgagtatgg
     1441 tttccccctc aagttaaaag cccgctatga caactttatt ggcggcgaat gggtagcccc
     1501 tgccgacggc gagtattacc agaatctgac gccggtgacc gggcagctgc tgtgcgaagt
     1561 ggcgtcttcg ggcaaacgag acatcgatct ggcgctggat gctgcgcaca aagtgaaaga
     1621 taaatgggcg cacacctcgg tgcaggatcg cgcggcgatt ctgtttaaga ttgccgatcg
     1681 aatggaacaa aacctcgagc tgttagcgac agctgaaacc tgggataacg gcaaacccat
     1741 tcgcgaaacc agtgctgcgg atgtaccgct ggcgattgac catttccgct atttcgcctc
     1801 gtgtattcgg gcgcaggaag gtgggatcag tgaagttgat agcgaaaccg tggcctatca
     1861 tttccatgaa ccgttaggcg tggtggggca gattatcccg tggaacttcc cgctgctgat
     1921 ggcgagctgg aaaatggctc ccgcgctggc ggcgggcaac tgtgtggtgc tgaaacccgc
     1981 acgtcttacc ccgctttctg tactgctgct aatggaaatc gtcggtgatt tactgccgcc
     2041 gggcgtggtg aacgtggtca acggcgcagg tggggaaatt ggcgaatatc tggcgacctc
     2101 gaaacgcatc gccaaagtgg cgtttaccgg ctcaacggaa gtgggccaac aaattatgca
     2161 atacgccacg caaaacatta ttccggtgac gctggagctg ggcggcaaat cgccaaatat
     2221 cttctttgct gatgtgatgg atgaagaaga tgcctttttc gataaagcgc tggaaggctt
     2281 tgcactgttt gcctttaacc agggtgaagt ttgcacctgt ccgagtcgtg ccttagtgca
     2341 ggagtcaatc tacgaacgct ttatggaacg cgccatccgc cgtgtcgaaa gcattcgcag
     2401 cggtaaccca ctcgacagcg tgacgcaaat gggcgcgcag gtttctcacg ggcaactgga
     2461 aaccatcctc aactacattg atatcggtaa aaaagagggc gcagacgtgc tcaccggcgg
     2521 gcggcgcaag ctgctggaag gtgaactgaa agacggctac tacctcgaac cgacgattct
     2581 gtttggtcag aacaatatgc gggtgttcca ggaggagatt tttggcccgg tgctggcggt
     2641 gaccaccttc aaaacgatgg aagaagcgct ggagctggcg aacgatacgc aatatggcct
     2701 gggcgcgggc gtctggagcc gcaacggtaa tctggcctat aagatggggc gcggcataca
     2761 ggctgggcgc gtgtggacca actgttatca cgcttacccg gcacatgctg cgtttggtgg
     2821 ctacaaacaa tcaggtatcg gtcgcgaaac ccacaagatg atgctggagc attaccagca
     2881 aaccaagtgc ctgctggtga gctactcgga taaaccgttg gggctgttct gatataagaa
     2941 gctggtcgca ttgggtattc attgcctgat gcgacgctta cgcgtcttat catgcctacg
     3001 ggaacctgac cgtaggccgg ataaggcgta cacgccgcat ccggcataaa caaagcgcac
     3061 gttgttacaa tttacgtttt aaacctacgt gcgcaataac ccagactacg ccaacagaag
     3121 tgcgtttctg gggcgtgttt tgtcaggagt ttaatctcgc gtttcgatgg tgagcgggcg
     3181 aaccaggccg taaatcacca ttggtgatcc gaggccaatc agcaataaac ggtagataat
     3241 tgaaggttta atacgcttaa tctgtactgt ggatgtttta ctggagttat tcattagttt
     3301 ggccaatagt tccactattt caatatgata aacctcgtcc tgttgaaaaa
//
LOCUS       DAZFRN010000286         3323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFRN010000286 DAZFRN010000000
VERSION     DAZFRN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.289"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            338..1654
                     /gene="shiA"
                     /locus_tag="ACHKUB_005025"
     CDS             338..1654
                     /gene="shiA"
                     /locus_tag="ACHKUB_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO6657900.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1756..3210
                     /gene="amn"
                     /locus_tag="ACHKUB_005026"
     CDS             1756..3210
                     /gene="amn"
                     /locus_tag="ACHKUB_005026"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO6657901.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          759 a          821 c          806 g          937 t
ORIGIN      
        1 ctcaacaatg atgctaaaaa catacctaac ctcgcctccc tactggttat aatacaatgc
       61 agtctatcag actcatcagg gtgccatttt gtgcatatgc ggacttttat gtttcatatc
      121 tctaacctgt gggtcctctg cttaatcctt aaacaacacc agcaactcct gcgctttcat
      181 cttccatcga atttttcatg ttgccgctaa tcagccataa aaacatttgc agatgcgctc
      241 tgtcgaggta gtctcataag gttcgtttat agatcgacgg caatgtgagt taccttttcc
      301 atactaatta taaaaagaca gtacaaacag gatcattatg gactccacgc tcatctccac
      361 tcgtcccgat gaagggacgc tttcgttaag tcgcgcccga cgagctgcgt taggcagctt
      421 cgctggtgcc gtcgtcgact ggtatgattt tttactctat ggcatcaccg ccgcactggt
      481 gtttaatcgc gagtttttcc cgcaagtaag cccggcgatg ggaacgctcg ccgcatttgc
      541 cacctttggc gtcggattcc ttttccgtcc gctcggcggt gtcattttcg gtcactttgg
      601 cgaccgactg ggacgtaagc gcatgttaat gctgaccgtc tggatgatgg gcatcgcgac
      661 agccttgatt ggtattcttc cttcattctc gaccattggg tggtgggcac ctattttgct
      721 ggtgacactg cgtgccattc agggatttgc cgtcggcggc gaatggggag gcgcggcgtt
      781 gctttccgtt gaaagtgcac cgaaaaataa aaaagccttt tacagtagcg gtgtacaagt
      841 tggctacggt gtaggtttac tgctttcaac cggactggtt tcattgatca gtatgatgac
      901 gactgacgaa cagtttttaa gctggggctg gcgcattcct ttcctgttta gcatcgtact
      961 ggtactggga gcattgtggg tgcgcaatgg catggaggag tccgcggaat ttgaacaaca
     1021 gcaacattat caagctgccg cgaaaaaacg catcccggtt atcgaagcgc tgttacgaca
     1081 tcccggtgct ttcctgaaga ttattgcgct acgactgtgc gaattgctga cgatgtacat
     1141 cgttactgcc tttgcactta attattcaac ccagaatatg gggttaccgc gcgaactttt
     1201 cctcaatatt ggtttgctgg taggtggatt aagctgcttg acaattccct gttttgcctg
     1261 gcttgccgat cgttttggtc gccgcagggt ttatatcaca ggtgcgttaa tcggaacgtt
     1321 gagcgcattt cctttcttta tggcgcttga agcacaatct attttctgga tagttttctt
     1381 ctccataatg ctggcaaaca ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt
     1441 taccgaaatg tttggtgcca gttatcgcta tagtggtgct ggagtcggtt atcaggtcgc
     1501 cagtgtggtt ggcggtggat ttacaccttt tattgccgct gcactcatca cttactttgc
     1561 cgggaactgg catagcgtcg ccatttattt gctggctgga tgtctgattt ccgcaatgac
     1621 cgctttgttg atgaaagaca atcaacgcgc ttgatagtct ggcgaagatc atccgatctt
     1681 cgccttacac ttttgtttca cattcctgtg acatactatc ggatgtgcgg taattgtatg
     1741 gaacaggaga cacacatgaa taataagggc tccggtctga ccccagctca ggcactggat
     1801 aaactcgacg cgctgtatga gcaatctgta gtcgcattac gcaacgccat tggcaactat
     1861 attacaagtg gcgaattacc tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca
     1921 tcactgaccg taacctggga cggtagcaca accaatcccc ccaaaacgcg cgcatttggt
     1981 cgttttactc acgcaggcag ctacaccacc acgattactc gccctactct ctttcgttcg
     2041 tatctcaatg aacaacttac gttgctgtat caggattatg gtgcgcatat ctcggtgcaa
     2101 ccctcgcagc atgaaatccc ctatccttat gtcatcgatg gctctgaatt gacacttgat
     2161 cgctcaatga gcgctgggtt aactcgctac ttcccgacaa cagaactggc gcaaattggc
     2221 gatgaaactg cagacggcat ttatcatcca actgaattct ccccgctatc gcattttgat
     2281 gcgcgccgcg tcgatttttc cctcgcgcgg ttgcgccatt ataccggtac gccagttgaa
     2341 cattttcagc cgttcgtctt gtttaccaac tacacacgtt atgtggatga attcgttcgt
     2401 tggggatgca gccagatcct cgatcctgat agtccctaca ttgccctttc ttgtgctggc
     2461 gggaactgga tcaccgccga aaccgaagcg ccagaagaag ccatttccga ccttgcatgg
     2521 aaaaaacatc agatgccagc atggcattta attaccgccg atggtcaggg tattactctg
     2581 gtgaatattg gcgtgggacc gtcaaatgct aaaaccatct gcgatcatct ggcagtgcta
     2641 cgcccggatg tctggttgat gattggtcac tgtggcggat tacgtgaaag tcaggccatt
     2701 ggcgattatg tacttgcaca cgcttattta cgcgatgacc acgttcttga tgcggttctg
     2761 ccgcccgata ttcctattcc gagcattgct gaagtgcaac gtgcgcttta tgacgccacc
     2821 aagctggtga gcggcaggcc cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg
     2881 gtaaccacag atgacaggaa ctgggaatta cgttactcag cttctgcact tcgttttaac
     2941 ttaagccggg ccgtagcaat tgatatggaa agtgcaacca ttgccgcgca aggatatcgt
     3001 ttccgcgtgc catacgggac actactgtgt gtttcagata aaccgttgca tggcgagatt
     3061 aaacttcccg gccaggctaa ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt
     3121 ggcattcggg cgatcgattt gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta
     3181 cgaaccttta atgagccgcc gttccgataa taagttctga accttcttat cagaaataga
     3241 tgtgaaggag taagtaagac acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt
     3301 ccttgggcgg atacgtgttt tat
//
LOCUS       DAZFRN010000287         3310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFRN010000287 DAZFRN010000000
VERSION     DAZFRN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.290"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(123..1610)
                     /gene="yeeO"
                     /locus_tag="ACHKUB_005027"
     CDS             complement(123..1610)
                     /gene="yeeO"
                     /locus_tag="ACHKUB_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO6657902.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            1771..1846
                     /locus_tag="ACHKUB_005028"
     tRNA            1771..1846
                     /locus_tag="ACHKUB_005028"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1804..1806,aa:Asn,seq:gtt)
     gene            2008..3282
                     /locus_tag="ACHKUB_005029"
     CDS             2008..3282
                     /locus_tag="ACHKUB_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6657903.1"
                     /translation="MSLNDSKIRKFKPSSRPVKLSDSHGLYLLVNPGGSRIWYLKYRF
                     NGKESRVSLGAYPLVSLAEARQQRDGIRKLLAQNINPAQQRMAEKAACSPEKCFKAVA
                     LAWHKTNKKWSADYAGRILASMENHIFPAIGHLPVATLKTQDFTALLRVIEDKGFLEV
                     ASRTRQQLSNIMRYAVQQGLTDSNPAQHLEGVTASPVKNHYPALPLERLPELLGRIGD
                     YQQGRELTRLAVVLTLHLFIRSSELRFARWSEIDFRHKIWTIPATREAIDKVRFSGRG
                     AKMRTPHIVPLSRQAIAILKQIQELSGHLDLVFPGDHNPYKPMSENTTNRALRLMGYD
                     TKTEICGHGLRAMACSALVESDLWSRDTVERQMSHQERNSVRAAYVHKAEHLEARKAM
                     MQWWSDYLDVCREGYVAPWIYAKQQSVSVDIL"
BASE COUNT          911 a          893 c          777 g          729 t
ORIGIN      
        1 ctaccgactg aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca
       61 taatgtcaaa gcctgttttt taaatttgaa atcgtttgct gaaataatct gcattttgtc
      121 gtttattccg acacagctgg ctttttttca cacttttgcg gctcggatcg agggtatttc
      181 catagccaac gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtcg
      241 gcaaacattc ccatccagac accaaccaca ccccagccaa gcatgatgcc cagcacataa
      301 ccgacgacaa cccggcaacc ccacatgctc aacatcgaaa cccacatggc gtaacgggca
      361 tcacgagcac ctttaaatcc agcgggtagc acccatgagg cggaccaaat aggcataaat
      421 aaagcattta gccaaatcag aatcacaacg acatgtttaa cctgtggatc ctgggtgtaa
      481 aacgatgcca taagcccggc aaagggagcc gttagccagg cgatggccgt taatccaaga
      541 gtggacagcc agaacacatg ccgcaactga atctctgctt gcgctatctg ccctaccccc
      601 aaccttcggc ctgtaatgat cgtagaagca gagccgagcg cacttccggg taagttgata
      661 agagccgcaa ttgaaaacgc gataaaattt ccggcaataa cactggtccc catcccggca
      721 acgaacattt gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgctgga
      781 ataccaatcc ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt
      841 aacgaaatcc ttagcgcagg attaaaacca atcgccagca cccacaaaat tgcaactgcg
      901 ccaatataac gagaaatggt taaacccaac ccagccccga caaatcccag tcccggccag
      961 gagaaaaggc cgtaaatcaa tatgccgcta ataataatat taagaatatt caggctaccg
     1021 ttaatcagta gcggtatttt cgtattccct gcaccacgaa gtgccccgct accaatcaaa
     1081 gtgatggcag ctgctggata actgagtacc gtcagctcca gataagtcaa cgccagtgct
     1141 ttaacttctg tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc aaaatgatga
     1201 ataagcgttg ccaacagtac ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc
     1261 accctcgctc gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac agtagtacca
     1321 agatcgatag ctgcaaaaaa agccataatg accatattga agctgtccgc caatcccacg
     1381 ccggccatcg catcttttcc cagccagctg accagaaaag tgctcaggac acccatcaac
     1441 aggacacagg cattctccat gaagatagga acagcaagcg gggttatctc gcgccagaac
     1501 aacactttgt agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac ctggcgtaaa
     1561 gcagaggaga tattcaaagc cgaccttaat tgcagaaagt gaaaccacat ttcaaataat
     1621 gagggagaat cagcaaagct gcaaagattt tcgccaacaa attgtctgca aatgcaacaa
     1681 actgttgata gaaacggcaa acagttgggg aatttaaaaa tcgggtttga caaaagattt
     1741 ttcgccgtta agatgtgcct caacagcgat tcctctgtag ttcagtcggt agaacggcgg
     1801 actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt caagttttca
     1861 tgcttatcta tgcatctcta tttgtttacg attcagtaag ttagcgtgaa aattcttccc
     1921 gatgcgtatc tggttttctc tccgcatcgg gataatttgg tggtcagatc tggggtcagt
     1981 ttggttcgat tctggagtga cccccaaatg tctttaaacg attctaaaat ccgcaagttc
     2041 aaaccctctt cccgtccggt aaaactctcc gactctcacg gtctgtatct gctcgtcaat
     2101 ccgggcggtt cacgcatctg gtatctcaaa tatcgtttca acggaaaaga atccagagtc
     2161 agtcttggcg catacccgct tgtctcactg gcggaagcca ggcagcagcg cgacggtatc
     2221 cgtaagctgc ttgcgcagaa tatcaacccg gcgcagcagc gcatggcaga gaaagccgcc
     2281 tgctcccctg aaaagtgctt taaagcggtg gcgctggcct ggcacaaaac caacaaaaag
     2341 tggtcggctg attatgccgg acgtattctc gccagtatgg aaaaccatat cttcccggcg
     2401 attggtcatc tgcccgttgc tacgcttaaa acgcaggatt tcacggcttt gttgcgggtt
     2461 atcgaggata aaggctttct ggaagtcgcg tcccgaaccc ggcagcaact cagcaacatc
     2521 atgcgttatg ccgttcagca gggacttacc gacagtaatc cggcgcagca tctggaaggt
     2581 gtaactgcct cccccgtcaa gaatcactat cccgctttac cgctggagcg attgcccgaa
     2641 ctgcttggcc gcatcggtga ctatcagcag ggccgggagc tgacccggct ggcggtggtg
     2701 ctgacgctgc acctgttcat ccgttccagc gaactgcgtt tcgcccgctg gagcgagatc
     2761 gatttcaggc acaaaatctg gaccatcccc gccacccgcg aggccatcga caaagtgcgg
     2821 ttttccggtc gtggcgcaaa aatgcgtact ccgcatatcg tgccgctctc ccgccaggcg
     2881 attgccattc tgaaacagat acaggaactt tccggccatc tggatctggt gtttcccggc
     2941 gaccataatc cgtacaagcc aatgagtgaa aacaccacca accgggcgct gcgtctgatg
     3001 gggtatgata cgaaaactga aatctgcggg catggactca gggcaatggc ctgtagcgcc
     3061 ctggtggagt cggatctgtg gtcacgcgat acggtggagc ggcaaatgag ccaccaggag
     3121 cgcaacagcg tgcgtgcggc atacgtgcat aaggcggagc atctggaagc ccgtaaggcc
     3181 atgatgcaat ggtggtctga ttatctggat gtgtgccgcg aggggtatgt tgcgccctgg
     3241 atatatgcca aacaacaaag cgtctctgta gatattctat aaaaccactg agtaaataat
     3301 attatttgaa
//
LOCUS       DAZFRN010000288         3288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFRN010000288 DAZFRN010000000
VERSION     DAZFRN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.291"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            182..472
                     /locus_tag="ACHKUB_005030"
     CDS             182..472
                     /locus_tag="ACHKUB_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657904.1"
                     /translation="MSKIFRQCMPYGIRKTAEGAWEAFNRDYQPLGEAFFFKRALTQA
                     TRDVLAPPPVTQRDDCVWLYSDIEHPMHSEAYWKAYCQRLKHLSNLKMKDER"
     gene            498..920
                     /locus_tag="ACHKUB_005031"
     CDS             498..920
                     /locus_tag="ACHKUB_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6657905.1"
                     /translation="MRRDMQLVLERWGRWAASEEYCSMVDWPSMSVTSRDVTDSGKPG
                     CSDEDGAVIDTCIAHMSMVCPYDDLLILGLRFIGGLSTRAIAEAIDRSHLSVRTSLKA
                     SEAFLEGALTLQGVRLDMDPEVVLPERVVCAQKNMLWL"
     gene            1000..1104
                     /locus_tag="ACHKUB_005032"
                     /pseudo
     CDS             1000..1104
                     /locus_tag="ACHKUB_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            1090..1166
                     /locus_tag="ACHKUB_005033"
     tRNA            1090..1166
                     /locus_tag="ACHKUB_005033"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1124..1126,aa:Arg,seq:tct)
     gene            1258..2217
                     /gene="stxA2b"
                     /locus_tag="ACHKUB_005034"
     CDS             1258..2217
                     /gene="stxA2b"
                     /locus_tag="ACHKUB_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO6657906.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFMIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            2230..2493
                     /gene="stxB2b"
                     /locus_tag="ACHKUB_005035"
     CDS             2230..2493
                     /gene="stxB2b"
                     /locus_tag="ACHKUB_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO6657907.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            3017..>3288
                     /locus_tag="ACHKUB_005036"
     CDS             3017..>3288
                     /locus_tag="ACHKUB_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO6657908.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATSDPGYYFVVVLAGQS"
BASE COUNT          875 a          717 c          833 g          863 t
ORIGIN      
        1 tgtattatgc gatctcttat gtatatagaa ctaaaactat ctgccacccg ccacccaaat
       61 agcccggcac cttacgtggt gtgcgtttca ggaggtggca gatagctatt tgagtgccac
      121 ctaactgcca cccggaaatt aacttgaaaa aattagcaaa taactaaatg aggatacgac
      181 gatgtcaaag atattccgcc aatgtatgcc ttacggtatc cgcaaaacag ctgagggggc
      241 ttgggaagca ttcaacagag actaccagcc cttaggggag gcttttttct tcaaaagagc
      301 actaacgcag gccactcgag atgtgttagc gccaccgccg gtaactcagc gagacgactg
      361 tgtatggctg tatagcgaca tcgagcaccc gatgcactct gaggcttact ggaaagcgta
      421 ttgccagcgg cttaaacatc tttctaacct caaaatgaaa gatgaacggt aagtagcaaa
      481 acgtagaggc cagcgttatg cgtagagata tgcaacttgt tctggagcgg tgggggcgat
      541 gggcggcgag cgaggaatac tgttcaatgg ttgactggcc ctcaatgtcg gttacttccc
      601 gggacgtaac cgacagtggc aaacccggtt gctctgacga ggacggagcg gtgattgata
      661 cctgcatcgc gcatatgagt atggtttgcc cgtacgatga tttgctgatc ctggggttac
      721 gttttatagg cggcctctca acgcgtgcaa ttgctgaggc gatagaccgt agtcatctta
      781 gcgtacggac atccctgaag gcgtctgaag catttctgga aggagctctg actctgcagg
      841 gagtaaggtt ggatatggat cctgaagttg tactacctga aagggttgtg tgcgcacaaa
      901 aaaatatgtt atggttataa cgtctataat tgtatgctta ctgctgaaag gttccgataa
      961 caccggaacc ttttttcttg tcttctgctg ggaaaaatta ataaaacagg aaagaaaaac
     1021 atgacatcgc actggttcag tgtcagccag tggcagttac caaacgagga tgattacaga
     1081 aaacttcatg cgttgttagc tcagccggac agagcaattg ccttctaagc aatcggtcac
     1141 tggttcgaat ccagtacaac gcgccatact tattttttct ggctcgcttt tgcgggcctt
     1201 ttttatatct gcgccgggtc tggtgctgat tacttcagcc aaaaggaaca cctgtatatg
     1261 aagtgtatat tatttaaatg ggtactgtgc ctgttactgg gtttttcttc ggtatcctat
     1321 tcccgggagt ttatgataga cttttcgacc caacaaagtt atgtctcttc gttaaatagt
     1381 atacggacag agatatcgac ccctcttgaa catatatctc aggggaccac atcggtgtct
     1441 gttattaacc acaccccacc gggcagttat tttgctgtgg atatacgagg gcttgatgtc
     1501 tatcaggcgc gttttgacca tcttcgtctg attattgagc aaaataattt atatgtggct
     1561 gggttcgtta atacggcaac aaatactttc taccgttttt cagattttac acatatatca
     1621 gtgcccggtg tgacaacggt ttccatgaca acggacagca gttataccac tctgcaacgt
     1681 gtcgcagcgc tggaacgttc cggaatgcaa atcagtcgtc actcactggt ttcatcatat
     1741 ctggcgttaa tggagttcag tggtaataca atgaccagag atgcatccag agcagttctg
     1801 cgttttgtca ctgtcacagc agaagcctta cgcttcaggc agatacagag agaatttcgt
     1861 caggcactgt ctgaaactgc tcctgtgtat acgatgacac cggaagaagt ggacctcaca
     1921 ctgaactggg ggagaatcag caatgtgctt ccggagtttc ggggagaagg gggtgtcaga
     1981 gtggggcgaa tatcctttaa taatatatca gcgatactgg gcacagtggc ggttatactg
     2041 aattgccatc atcagggggc gcgttccgtt cgcgccgtga atgaagagat acaaccagaa
     2101 tgtcagataa ctggcgacag gccagttata aggataaaca atactttatg ggaaagtaat
     2161 accgcagctg cttttctgaa tcgcagggcc cactctttaa atacatccgg agaataacag
     2221 gagttaaata tgaagaagat atttgtagcg gctttatttg cttttgtttc tgttaatgca
     2281 atggcagctg attgtgcaaa aggtaaaatt gagttctcta agtataatga gaatgataca
     2341 ttcacagtaa aagtggccgg gaaagagtac tggactaacc gctggaatct gcaaccgcta
     2401 ctgcaaagcg cacagttaac aggaatgacg gtaacaatca aatcaaatac ctgtgcgtca
     2461 ggttcaggat ttgctgaagt gcagtttaat taatatcaga agcattgctg gtttcgtggt
     2521 gtgcagcaat gtagttacag tgtaatcaat gtcacaattc agtcagttga aggttgtctg
     2581 cccgactgag aatttgttaa aaaaaatcct gcatggtgaa tccccctgag cggcggggca
     2641 tatcagcgtc acaggtgttt ctgttttacc tctatccttt ctgtgcgggt tcaggtgctg
     2701 atactgaact caccgggagg cacccggcac catgcatgaa cggtacatag cgcatacatc
     2761 agcccctctc cggaggggct ttcttgtggg caaaaaaaaa agcccgcgcc gggagacgcg
     2821 ggcggcaagg aataaacaac aaaacgtgaa gtaatatttc agctggcgaa taatacccga
     2881 cagtaatcac tctgcgcaac tgcgtggcct ttttcgtatt gcgggctgta gtcttcctcc
     2941 tgtcattgtc ctgtaacttc cggacttcag cccgctcctt atccgactaa caacattatc
     3001 ccgaccggga ggattcatga catttaaaca ttacgatgtg gtcagggcgg catcgccgtc
     3061 agaccttgct gatgcacttg cgcaaaaaat tcgtgaagga tggcaaccat acggtgggcc
     3121 gttttcttcg tatacggatg atggtgcagc acttattcag gcgattgtcg cagaaggtga
     3181 tgtgagcaca cctgttgtgg tgaagccgac aggtggagaa ggtgcagtaa tcagcgccac
     3241 cagcgacccc gggtattact ttgttgtggt tctggcaggg cagtcaaa
//
LOCUS       DAZFRN010000289         3261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFRN010000289 DAZFRN010000000
VERSION     DAZFRN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.292"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(55..1551)
                     /locus_tag="ACHKUB_005037"
     CDS             complement(55..1551)
                     /locus_tag="ACHKUB_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO6657909.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGS
                     GNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDV
                     QGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVG
                     SEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVD
                     VEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNE
                     EPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKSLAR
                     EIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADRDALVNAWRSRDAQDIANK
                     LGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMTLLS
                     AIIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINNKLVHFTH"
     gene            1790..2080
                     /locus_tag="ACHKUB_005038"
     CDS             1790..2080
                     /locus_tag="ACHKUB_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657910.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            complement(2072..2350)
                     /locus_tag="ACHKUB_005039"
     CDS             complement(2072..2350)
                     /locus_tag="ACHKUB_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657911.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRASLAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            2471..2893
                     /locus_tag="ACHKUB_005040"
     CDS             2471..2893
                     /locus_tag="ACHKUB_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO6657912.1"
                     /translation="MRSAVARLLLIPLITATIVLTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGVKDKAAGVSAETAQQRIKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
BASE COUNT          969 a          813 c          571 g          908 t
ORIGIN      
        1 ttggttccta taggtcgttt aaagagaagt gatatgatca cctctctttt tctatcaatg
       61 agtaaaatga accaatttat tatttatttt ttcagccaca cttgcatcaa tatatgatgc
      121 aaggtaacta attcccataa tacctgcaat atttattgca gttaatgcta acgccggtag
      181 tgtgaatgat ccaattatag cactaagcaa tgtcatggca acgcttgcgg ccataccact
      241 gagaacccaa gactcaacct ccaacatcag cggcccccag tttccagttt catatccctc
      301 gatgcttttc tccttgacct tctcaatctt cataattaca tcggcagctt tgaaagcctt
      361 actgatattc cccagtttat ttgctatatc ctgagcatca cgacttcgcc aggcgttcac
      421 cagtgcatca cggtctgctc tgttaacttt aaaactagga ttagacaaaa ttttatttaa
      481 tgactccatt gcatcatcgt agctacggat ggttttcccc tggaaatttt tgatatcatc
      541 agcaatttct cttgctaacg acttataaga ttcaccaaga taatcaccga tcttgttacc
      601 catatcagat ataagttcag ctgctttatt tagcacatca ctctgaatcc ggtcacggtt
      661 catactgaaa gaataaaata catcataagc agctttttta gcttgattct gtcgttgagg
      721 ctcttcatta ttaaatccta atgcctcgta tatctcttcc ggggtcttat ggtcgacttt
      781 atccataata ccagccttag ctttttcact ccctacataa ggacgaacag ctgttctagt
      841 tacagataaa acattaccat aatcatcaag caccactaaa tagtggaact gttcaacatc
      901 aacatcaaat tttctcacat taggaggcaa acgatcccac gcatcttcag cctgttgttt
      961 tgctttttcc tgccgctgag attcatcttt attaaaacct aacgcctcat atatctcctc
     1021 aggtgtttta tgattgattt tttccataat acccgcctta gctttttcac tccctacgta
     1081 aggacggaca cctgttcgaa gaataccgat agctctacca ttatcattaa gcgttacacg
     1141 atatttaaaa ccatcgatac tcacatcaat ctctcgcggt aagttgtttt tatcccactt
     1201 acgagcaact tcatcattag gattttcagg aatatagccc ccctgaacat cactatttcc
     1261 gccagcctca ggaactccat atacaggcgt ttttaccaga ccatttgtaa tggtaatgcg
     1321 cacctgccca ttatcatcaa cataaggcgg cacatccgga ttattggttt tactccatcc
     1381 ccaccccgtc tgacttccat gttcaccacc tccattacca gagcctgaac caccacccca
     1441 gtgaacccca ctattactat tattatttcc accttgctga ttcagattcg cccctgttcc
     1501 ccccatagac tcaccagcag ttgggccata tccacttagt tctttagcca taaattcctc
     1561 tttgataatt aaaacaataa attaaaaaca acacactgta catataacca ctggttttat
     1621 gtacagaaaa acctactact caccattgtc catgtcaaga gcatggattt tcatttttgc
     1681 aataaggatc acactatggg gaggcaggca ttgagaacgt cgaaacagaa caccggagca
     1741 aatcaggatg agatataaaa ctgttggatc atgaaaaacg gagaacgatg tgagcaaatc
     1801 accccgccat aaactgaaca aaacagacaa acgacttctc gacacccttg ttgctgccgg
     1861 atatgagcat gacaaagccc gtgacctcat ccagaaacag gtttacacgc tgacactggc
     1921 tgatcagcgc catgtggtca gtgaaatcag taacggtgtg aatcccaccc aggcttactc
     1981 ggcggtatac caggcaagac gcattcgcct cgcccgtaaa tatctgaacg gtaaaaaggt
     2041 tatggaagaa accggggaaa atacgccccc atcagcgtaa ggatttcttt tgccgctcca
     2101 gagactccag ttttttacgc aaatcctctc ttttttgggc atctctggtg ccagccagag
     2161 atgccctcaa ctcatcgatc tgaagttgta tcttcagtct gttactgaac attttctgtc
     2221 tggcattaac atccgcaaca atgccatttt ttgtcttctc ggccttttgt tgaaaaacat
     2281 tgctgtccgc atgactggca accgaagcag aaagaacact aaaaagcagg actggcacac
     2341 attttttcac gggattattc ctgactcatt gaccatcaaa tcacattggg gtaaaccgac
     2401 gtatgataag agatactctt cggaaatata actccctgag tatcaagatt aaaaacgcaa
     2461 ggagatgttt atgagatctg ccgttgccag gctgcttctg atacctctga taacagcaac
     2521 aatcgttctt acaggatgca caccaaagac cagcctggaa cgacataccc ggcattatgt
     2581 ttatgcttca gatgatggat ttgatcctaa cttctacacc cagaaagcag acaccatacg
     2641 tatgatgctc ccgttctttc agcagttccg ggatatgggg gtgaaagaca aagcagccgg
     2701 agtatcagca gaaacggcac agcaacgtat aaaagaattc cactcagaaa aattttttca
     2761 ctcactccgg agcacaacaa cctttgctgg cagaaaatac acaaacagcg atatgccgtc
     2821 gccgaaaaaa atgaaactaa tggcagacac catttctgcg gtttatctcg atggatacga
     2881 gggcagacag taaggggatt taccataatc ccttaattgt acgtaccgct gaaacgctct
     2941 catacgcgtt ctgacgcaaa aacgcaaaaa gacaaggaaa ccccaggcta aaccgaaaaa
     3001 atgcgtcaga atcgttttca ggtacttgca taactattca caaaagcgat gcgcgatcga
     3061 tttcgactat tccgacggac gaacaggtta aaatgataag tttcctccct ccggatggaa
     3121 ggttcagcac ggggattttt acccccttcc atcaacctga atatgcatct gggagtaccc
     3181 attagtttct actcaaacac tatcgtagtg ctatctcaaa atataaaaca taccgatggc
     3241 gtaagcattt taaatatgat a
//
LOCUS       DAZFRN010000290         3255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFRN010000290 DAZFRN010000000
VERSION     DAZFRN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.293"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            1..149
                     /locus_tag="ACHKUB_005041"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHKUB_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163699.1"
                     /note="catalyzes the formation of glutamate from
                     glutamine; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ACHKUB_005042"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ACHKUB_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO6657913.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /gene="digH"
                     /locus_tag="ACHKUB_005043"
     CDS             1828..3147
                     /gene="digH"
                     /locus_tag="ACHKUB_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO6657914.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSLLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          788 a          812 c          796 g          859 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg acaatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaacccac gtcaatgaaa
      841 tgagtaaccc agggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc tctggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taagtcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact agttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcggctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgcgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgcc gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatacggag tcaccgggtt cactgctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggtgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccattctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttacc ctcttgaact atcccactac taatcatgct tactaaagtc
     3241 aaattaacca cgcaa
//
LOCUS       DAZFRN010000291         3211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFRN010000291 DAZFRN010000000
VERSION     DAZFRN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.294"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHKUB_005044"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHKUB_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO6657915.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHKUB_005045"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHKUB_005045"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO6657916.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="ACHKUB_005046"
     CDS             1252..1521
                     /locus_tag="ACHKUB_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO6657917.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="ACHKUB_005047"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="ACHKUB_005047"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO6657918.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..>3211
                     /gene="dsbA"
                     /locus_tag="ACHKUB_005048"
     CDS             2601..>3211
                     /gene="dsbA"
                     /locus_tag="ACHKUB_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO6657919.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYL"
BASE COUNT          807 a          775 c          785 g          844 t
ORIGIN      
        1 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagcttagag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      661 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctgct tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
      901 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatggcgatt
     1021 ggcattaacc acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agccacgcaa gctgtttgag gacgctacac tgatgccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2161 ctggcgggaa gacttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctgc gacaaacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttacaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgtccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt cactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggtgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gcatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgataca gtgaaatatc t
//
LOCUS       DAZFRN010000292         3178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFRN010000292 DAZFRN010000000
VERSION     DAZFRN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.295"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..165)
                     /locus_tag="ACHKUB_005049"
     CDS             complement(<1..165)
                     /locus_tag="ACHKUB_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657920.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIE"
     gene            complement(383..817)
                     /locus_tag="ACHKUB_005050"
     CDS             complement(383..817)
                     /locus_tag="ACHKUB_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657921.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTALPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
     gene            complement(1039..2139)
                     /locus_tag="ACHKUB_005051"
     CDS             complement(1039..2139)
                     /locus_tag="ACHKUB_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hsdR"
                     /protein_id="HFO6657922.1"
                     /translation="MFDVDDTAPDTPAPRELSQDVQALINNGLDFLDKAREELEASKP
                     KFSVVSFWTAVEILLKVPLAHEHWSLVCSPKKPIKKQAYLAGDFQSVTYEETRERLKD
                     VLERPLDRETDSAFDKVRKHRNRVVHFYHPTFTEAEQRQILKEQADAWFALNRLLRDE
                     WKVIFGVKHNWKLAFGETRLIRGNKFYAEVRFKQVKPELEQLSEKNIQIGNCNECHQH
                     ATVTGTETTGNENRKLEVTRCKVCTSAVRQITLVCPDCEIPQLLPEGDSDFECEHCDY
                     TSDRYKLLDEELFHSVDEQLLSVFPAGCTNCMTPESVCKFGDGYLCTQCFIYYTELHV
                     CGCCGHLSDSVPELSHIRGCEFCDGDQRYFDD"
     gene            2538..3140
                     /locus_tag="ACHKUB_005052"
     CDS             2538..3140
                     /locus_tag="ACHKUB_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6657923.1"
                     /translation="MNTSPWNKDRIIGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGSSVSSRATVLQQKTGSPVQFEITKGTREAVSALIKL
                     GNLRSKDYLFRSRVGTNQHISTRQYNRIFHGWVAKLGLEDSLYSTHSLRRTKPYLIYK
                     KTKNLRVIQLLLGHKKLESTVRYLGIEVDDALEISESIEV"
BASE COUNT          856 a          768 c          735 g          819 t
ORIGIN      
        1 ctcaattctt ttcagtgctt tctgtttatc aaaaagcagt ctgttttcgt tcttactgtc
       61 agataacaca tctggctcct caaccatttg ccggagcaga ttaatgttca gttgcaaaag
      121 tcggtcggct tcatcacaaa gcagggcgat gttcttttcg tgcattattt aaccttcctg
      181 agacgcattt tcatgacaac cacggaggag gtcatttact acaaacgatc gtttataacg
      241 atcgatatga tagtaacgat cgatgtattc tattaacttg ggaagcacaa cgcaagggga
      301 caccccgggt atggctgcgt ttgcatagtg ttttcatgga gttaaaaatc tgtaacaaca
      361 gcctggtaca tcaccaccgg gttcagaaga aaatccagtt ccataccgcg cgggcaacag
      421 agaccaccgt gtcacgaact gcgcgcaggg ccgtacggac aacagaggca acacagacac
      481 tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg gtctgagtgc
      541 ggacagactc cgtgcgcagt tcgtcctgca ggcgtgtcat cagagggatg gcggcatgtc
      601 cgggaagcgc tgtcatgagt gcactgaccg gggtgacctc cccctgaaac agtgcattac
      661 agagtgagga tttaccagcc ccgcttttac ccataatgcc aggcacgggt tcgtggctgg
      721 tgagtttgcg cagatgttcc aggatgtgac gggaaagtga gtaaggcagg gaggagagcg
      781 gtttttcaat tgcctcaatg gcatcagacg gattcataca tgtattcccg gtgaaaaaag
      841 acaaaacccc gcgcaccgga aaggtggcgg ggggattcac ggagataata tctgcctgaa
      901 attatttgga atgcaggtat atgtggctga tttgtatcag ctaatgagta caggtctgaa
      961 tctaatctga cagtccgctc tgtgccagga gcggactgtc caacccatcc ctgaagagcc
     1021 tagattatgt atggtaattc agtcatcaaa ataacgttgg tcaccatcac aaaattcaca
     1081 tcctcggata tggctcagtt caggaacgct atcgctcagg tgaccgcaac aaccacatac
     1141 atgaagctcg gtgtaataga tgaaacactg agtgcaaaga tagccgtctc caaatttaca
     1201 tacgctttca ggagtcatgc aattggtaca tccagcagga aatacggaga gtagctgttc
     1261 atccacactg tggaacaact cctcatccag cagtttatac ctatccgaag tgtagtcgca
     1321 gtgttcgcat tcaaaatcac tatctccctc aggtagcaat tgaggtattt cgcagtctgg
     1381 acaaactagg gtaatttgcc ttacagcact ggtgcaaacc ttacaccgcg taacttcaag
     1441 cttacggttt tcattaccgg tcgtttcagt ccctgttacc gtcgcatgct gatggcactc
     1501 attgcagtta ccgatttgaa tattcttttc agagagctgc tcgagttcag gcttaacctg
     1561 tttaaaccgc acctcggcat aaaatttgtt tcccctaatc agacgagttt caccgaaagc
     1621 cagcttccaa ttatgtttga caccgaagat aactttccat tcatccctta ataaccggtt
     1681 cagggcaaac caggcgtcag cttgttcttt gagtatttga cgttgttcag cctcggtgaa
     1741 agtggggtgg tagaaatgaa ctacgcggtt gcggtgcttt ctgaccttat caaaagcact
     1801 gtccgtttct ctgtctaggg gtctttcaag tacgtcctta agcctttcac gggtttcttc
     1861 gtaggtcacc gactgaaaat caccggctag gtaagcctgc ttttttattg gctttttcgg
     1921 gctgcagacc aacgaccagt gttcgtgcgc cagcggaact ttcagtaaaa tctcgaccgc
     1981 agtccagaaa ctgacgacgg agaacttcgg cttcgaggcc tccaactctt cacgggcttt
     2041 atccagaaaa tccagaccat tgttgatcag cgcctgcaca tcctgactga gttcacgggg
     2101 ggcgggagta tcgggtgcag tatcgtcaac atcgaacatc attatctcct ttaatcagct
     2161 tccaccacgc agttgctaca gcgcgttagt tgtatccagt gtaaactgga cttggtagct
     2221 ggcagccaca tcgagcgcaa aaaaccgtaa attaacttct gtagactcgt acttttcgtg
     2281 acccatgcgt acccgcagga tcttcgggtg cacgtgacca taaagcaggt gcctaccaaa
     2341 gctgtggcga ttcagatccc taaactgatg gggtcccaaa aagtatgcca tagcaagtgt
     2401 aagtatccct aaaatctgca ctattctctt tttataaatc ctctgacatg tgaattagat
     2461 tgccctaaag tgagcttccg cgcctcgctc ataaggacaa ccatactcaa atctcccaca
     2521 ttgcaggaga tttgagtatg aacacgtcac cgtggaacaa agaccgtatc ataggccaaa
     2581 aaagaccact tcagatatct catatctggg gtatccgaat ccgacttgaa ctggaaggta
     2641 aaacacgcga tttagctctg ttcaacatgg ccttggacag taagcttcga ggctgtgatc
     2701 tagtcaaact caaagtatct gatgttgcat atggtagctc tgtttcaagc agagctacgg
     2761 ttctacaaca gaaaaccggc agccctgtcc aatttgagat aaccaaaggg acaagagaag
     2821 ctgtttctgc attgataaag cttggcaatt tacgcagtaa agactatttg ttccgatctc
     2881 gggtcggtac taaccaacac atatcaaccc ggcaatacaa ccgaattttt catgggtggg
     2941 tagcaaaact tggtctcgaa gattcgcttt acagtacaca ttccttgaga agaacaaaac
     3001 cttacctgat ttacaagaaa actaagaatc tcagggtgat ccaacttctg ttaggccata
     3061 agaaactgga aagcacagtc cgttatctgg gcattgaagt cgatgatgcg ttagagatct
     3121 ctgaatcgat tgaagtctaa ggttgccagg gctgcaacag cagtcctgtg ccagatgc
//
LOCUS       DAZFRN010000293         3151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFRN010000293 DAZFRN010000000
VERSION     DAZFRN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.296"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            222..1154
                     /gene="rfaD"
                     /locus_tag="ACHKUB_005053"
     CDS             222..1154
                     /gene="rfaD"
                     /locus_tag="ACHKUB_005053"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO6657924.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            1164..2210
                     /gene="rfaF"
                     /locus_tag="ACHKUB_005054"
     CDS             1164..2210
                     /gene="rfaF"
                     /locus_tag="ACHKUB_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO6657925.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2214..>3151
                     /gene="rfaC"
                     /locus_tag="ACHKUB_005055"
     CDS             2214..>3151
                     /gene="rfaC"
                     /locus_tag="ACHKUB_005055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO6657926.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTV"
BASE COUNT          779 a          777 c          845 g          750 t
ORIGIN      
        1 cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac atgcaaaacc
       61 aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt agcatccttg
      121 cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca gacattcgtg
      181 tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg
      241 gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc atcaccgata
      301 ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctgaata
      361 tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg
      421 gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag tgggacggca
      481 agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac tgcctggagc
      541 gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc acctccgact
      601 ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca aaattcctgt
      661 ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt ggcttccgct
      721 atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc
      781 atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact
      841 ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg ttcctggaaa
      901 atggcgtttc cggcatcttc aatctcggca ccggtcgtgc ggaatccttc caggctgtag
      961 ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg ttcccggata
     1021 agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg cgcgcggcgg
     1081 gttacgacaa accgttcaaa accgttgctg aaggtgtaat ggaatacatg gcctggctga
     1141 atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct tgggttggcg
     1201 acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa
     1261 tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg
     1321 aagctatccc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg
     1381 gtcatagcct gcgtgaaaag cgctacgacc gcgcctacgt cttaccaaac tccttcaaat
     1441 ctgcattagt gcctttcttc gcgggtattc ctcatcgcac tggctggcgc ggcgagatgc
     1501 gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg
     1561 aacgctatgt cgcgctggcc tatgacaaag gcattatgcg taccgcacaa gatctgccgc
     1621 agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc
     1681 aattttcgct ttcatcagaa cgtccgatga ttggcttttg cccgggtgcg gagtttggtc
     1741 cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg attgatgaag
     1801 gttatcaggt ggttctgttt ggctctgcga aagatcatga agcgggcaat gagattcttg
     1861 ccgctttgaa taccgagcag caggcatggt gtcggaacct ggcgggggaa acacagcttg
     1921 atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat tctggcctga
     1981 tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtcca agtagcccgg
     2041 acttcacacc gccgctatcc cataaagcgc gcgtgatccg tctgattacc ggctatcaca
     2101 aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc
     2161 agcgcgtact ggaagaactc aacgcgctat tgttacaaga ggaagcctga cggatgcggg
     2221 ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg
     2281 atgcccagca ggcaatccca gggattaagt ttgactgggt ggtggaagaa gggttcgcac
     2341 agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata cgtcgctggc
     2401 gtaaagcctg gttctcggcc cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac
     2461 aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcactgg
     2521 tgacacgtct ggcgcatggc gtaaagcatg gattggactg gcaaaccgct cgcgaacctt
     2581 tagccagcct gttttacaat tgtaagcatc atattgcaaa acagcagcac gccgtagaac
     2641 gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc cagggcgatt
     2701 atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat
     2761 ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg cgagaattga
     2821 ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg
     2881 aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga
     2941 gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg
     3001 ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat ggaccaaccg
     3061 atccgggatt aattggtggg tatgggaaga atcaagtaga gtgtcgttct acaagtatgt
     3121 ctcttgcaga tttgccagct caaaccgttt t
//
LOCUS       DAZFRN010000294         3113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFRN010000294 DAZFRN010000000
VERSION     DAZFRN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.297"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..91
                     /locus_tag="ACHKUB_005056"
     CDS             <1..91
                     /locus_tag="ACHKUB_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657927.1"
                     /translation="GWSLCFWHVSVRMTHDVVGLLLLTDLIQQ"
     gene            complement(150..281)
                     /locus_tag="ACHKUB_005057"
                     /pseudo
     CDS             complement(150..281)
                     /locus_tag="ACHKUB_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            563..1921
                     /locus_tag="ACHKUB_005058"
     CDS             563..1921
                     /locus_tag="ACHKUB_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO6657928.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGLDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(2217..>3113)
                     /locus_tag="ACHKUB_005059"
     CDS             complement(2217..>3113)
                     /locus_tag="ACHKUB_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO6657929.1"
                     /translation="FRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLT
                     SKYAEHTPLYCQSEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALHGYVMTDGKLHA
                     DDTPVQVLLPGNKKTRTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTHLACF
                     SGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQLYA
                     IEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLFFGSGHGGERGALLYSLIG
                     TCKLNDVDPESYLRHVPGVIAD"
BASE COUNT          746 a          827 c          845 g          695 t
ORIGIN      
        1 gggatggagc ctctgcttct ggcatgtgtc ggtcagaatg actcatgatg tggttggtct
       61 gctattattg actgatctta tccagcaata atggacacgc ggctaagtga accgccccgg
      121 gtttcctgga gagtgtttta tctgtgaact caggctgcca gatcatcgtt tccgatggaa
      181 gcataataag ctttttctgc ttctgccggt gggatatggc cctgcctttc cagcaatcgt
      241 cgattgttat accagtccac ccacgtgagt gtggtcagct catcatactg acacgtgcat
      301 tacccctacc gcgataatgc aaccgtacaa tatagggcgt ttcctaaacg cagccctcat
      361 cactccaatc caacaagaca cagcattttt gatcacgaag aaagacaaat aatttccgta
      421 aatgtttccg ttgttacaaa taacaacagt gttttacatt tctttggcct caggcccgcg
      481 catgttgcca ttcgatgtca ttttcctgac tcgtttcttc tttatggtgt agtgtgatct
      541 tcactttatg gatgctaaca atatgaaaag aaaaattatt cccgtgctaa tcggttgtac
      601 gctctctttc tctgccctgg cggcacaacc tacggctgag cgctatgtcg tcagttttcc
      661 agaaggcacg cacgtgaact acgccggcgc atttgccagt gcattcccga acggccttcc
      721 ggttgggata ggttctggcc tgctgttcac cggtaaacag ggcgatgccc tgacattcgc
      781 aaccattacc gatcgcggtc cgaacgcgga ttcaccgaaa gaggggaaaa acgaaactaa
      841 aattttcgtt acgccagatt tcgcgccgct gctgatgacg attcgtgtac aaaacggcaa
      901 agcggaggcc atcgatcccc gtcctctgca tgacgataaa ggcgcgatca acggtctgcc
      961 gctggcaagc gacgtgattg gctccaccaa tgaagtggcc ttcagcgaca cgttacatag
     1021 actgaagggg gataatcgcg ggctggatac ggaagggatc actccggatg gtaaaggagg
     1081 ttactggctg tgcgatgagt atggcccgtt cctgattaac atcgacagta aagggaaaat
     1141 cctggcaatc cacggtccgc aggcggcgga aggggaaaaa gcgattgcgg gcggtttgcc
     1201 aaatatcctc aaatggcgtc aggcgaaccg cggctttgaa ggcctcaccc gtatgccgga
     1261 cgggcgtatt attgtcgccg tgcaaagtac gctggatatc gacgctaaga gcaagaaaaa
     1321 ggcgctgttt acgcgtctgg tgagcttcga tccggcaagc ggaaaaaccg cgatgtacgg
     1381 ttatcctatc gacagcgcgg cctacagcaa aaacagcgac gctaaaatcg gagacatcgt
     1441 ggcgctcgat aaccagcaca tcctgctgat cgaacaggga agagataaaa acaacagaat
     1501 gcgtaacctg atttacgagg tcgatctcaa taaggcgagc gacctgtcag gcttagacaa
     1561 gccgggtgag tatccggagt ttgatgatga gaaaaccctg tcacagcgcg gcattaccct
     1621 tgcgcagaaa acgcaggttg ttgatctgcg ctccttgggc tggcagcagg agaaggctga
     1681 agggttggcg ctgatcgaca gcaaaacgct ggcggtggcg aacgataacg actttggcgt
     1741 gaaggtggcg atgcaacatc cggtcgaggg caagacattc aaggattatc gggtgaacgc
     1801 ggaaggaaag ctgacgcttg acgacaagca ggttgaaacg accctcagag tgaagccact
     1861 ggagaaaccg gagtctgaca gtgaactgtg gattgtgacg ctgccggaag cgttgaaata
     1921 agtttataag gtacggacag cagtatcgct tagtttttca aattcatgta tgtgaacaat
     1981 attggggttc cggcgtagac ccccaatgaa agcgtcacca cgtgaaaaca tgcctgcacg
     2041 gtttacacgt catctcatgg tggcagccgg ttaactttcc tgctacacca gtggaaaaag
     2101 gccataagca gtccgaacaa aagtaagcgt acagcgaggg ccgtattgac ggggatgtgt
     2161 tattcagctg gcagtgctat gcgccacgga agcagttcgc tgacccggtt gaccggtcag
     2221 tctgctatga cgccaggcac atggcgaagg tagctttctg gatccacgtc attcagtttg
     2281 cacgtcccga tcaggctgta cagtagcgct ccccgctcac caccatggcc agagccgaag
     2341 aacagggtct tcatcttttc acgcaaccag ctttccaggg atttcaacag cggtttcgtt
     2401 tttcgctgac gttcagcaag ccgctgctct gccggcattc cccttatatc cgcctctatg
     2461 gcgtacaact gaccgatctg ctccagggct tcttccgtca gtgctgacgg gatgcggacg
     2521 tgcacatcgt ggatctttcg gcgggcatga gcccagcagg cagcttccgt tatcccacca
     2581 ttgcgataca gctcgttgaa cccggcgtac gcatccgctt gcagcacacc gctgaagcaa
     2641 gcaagatgag tctgcggatg gatgcctttt ctgtccgggc tgtaagcgaa ccacactgca
     2701 ggtgccaacg ctgacccggc attgcggtca tcacgaacat acgcccacaa ccgcccggtc
     2761 ctcgtcttct tattacccgg cagcagtacc tggaccgggg tatcatcggc atggagtttg
     2821 ccgtcagtca tgacatagcc atgaagcgcc tcttccagcg gagacagcag ccggcagcat
     2881 gcatccaccc agcccgacag cagtgaacgg ctcagctcca caccttgccg gccgtatatt
     2941 tctgactggc aatacagcgg ggtgtgctct gcatacttcg aggtcagcac gcgggccagc
     3001 agccccggtc cggcgatacc ccgctcgatg ggccgcgaag gtgcaggtgc ctgcacgatg
     3061 gcatcgcact gagtacaggc atgtttttcc cgtaccgtcc ggataacccg gaa
//
LOCUS       DAZFRN010000295         3096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFRN010000295 DAZFRN010000000
VERSION     DAZFRN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.298"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            50..1645
                     /gene="ydiF"
                     /locus_tag="ACHKUB_005060"
     CDS             50..1645
                     /gene="ydiF"
                     /locus_tag="ACHKUB_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO6657930.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            1659..2810
                     /gene="ydiO"
                     /locus_tag="ACHKUB_005061"
     CDS             1659..2810
                     /gene="ydiO"
                     /locus_tag="ACHKUB_005061"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO6657931.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2853..>3096)
                     /locus_tag="ACHKUB_005062"
     CDS             complement(2853..>3096)
                     /locus_tag="ACHKUB_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6657932.1"
                     /translation="SPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLF
                     LRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLVG"
BASE COUNT          833 a          712 c          794 g          757 t
ORIGIN      
        1 aattctccat ctggcgtagg gaaaatcaca tctgaatcag gaattaacaa tgaaacctgt
       61 aaaaccacct cgtattaatg gacgagtgcc ggtcctgtcg gcacaggaag cggtgaatta
      121 tattcccgac gaagcaacac tttgtgtgtt aggcgctggc ggcggtattc tggaagccac
      181 cacgttaatt acagctcttg ctgataaata taaacagact caaacgccac gtaatttatc
      241 gattattagt ccgacagggc ttggcgatcg cgccgaccgt ggtattagtc ctctggcgca
      301 agaaggtctg gtgaaatggg cattatgtgg tcactgggga caatcgccgc gtatttctga
      361 actcgcagaa caaaataaaa ttattgctta taactatcca caaggtgtac ttacacaaac
      421 cttacgcgcc gccgcagccc accagcctgg tattattagt gatattggca tcgggacatt
      481 tgtcgatcca cgccagcaag gcggcaaact gaatgaagtc acgaaagaag acctgattaa
      541 actggtcgag tttgataaca aagaatatct ctattacaaa gcgattgcgc cagatatcgc
      601 cttcattcgc gctaccacct gcgacagtga aggctacgcc acttttgaag atgaggtgat
      661 gtatctcgac gcattggtta ttgcccaggc ggtgcacaat aacggcggta ttgtgatgat
      721 gcaggtgcag aaaatggtta agaaagccac gctgcatcct aaatctgtcc gtattccggg
      781 ttatctggtg gatattgtgg tggtcgatcc ggatcaaagc caactgtatg gcggtgcgcc
      841 ggttaaccgc tttatttctg gtgacttcac ccttgatgac agcaccaaac ttagcctgcc
      901 cctaaaccaa cgtaaattag ttgcgaggcg tgcattattc gaaatgcgta aaggcgcggt
      961 ggggaatgtc ggcgtcggta ttgctgacgg tattggccta gtcgcccggg aagaaggttg
     1021 tgctgatgac tttattctga cggtagaaac aggtccgatt ggcggaatta cttcacaggg
     1081 tatcgccttt ggcgcgaacg tgaatacccg cgccattctg gatatgacgt cccagtttga
     1141 tttttatcac ggtggtggtc tggatgtttg ttatttgagt tttgctgaag tcgaccagca
     1201 cggtaacgtc ggcgtgcata aattcaatgg caaaatcatg ggtaccggtg gatttattga
     1261 tatcagtgcc acctcgaaga aaattatttt ttgcggcacg ttaactgcgg gcagtttaaa
     1321 aacagaaatt gccgatggca aattgcatat cgtccaggaa ggacgggtaa ataaatttat
     1381 tcgggaacta ccggaaatta cttttagcgg aaaaatcgcg ctcgaacggg aactggatgt
     1441 tcgttacatc accgaacgtg ctgtattcac gctgaaagaa gacggcctgc atttaatcga
     1501 aatcgcccct ggcgtcgatt tacaaaaaga tattctcgac aaaatggact tcaccccagt
     1561 gatttcgcca gaactcaaac tgatggacga aagattattt atcgatgcgg cgatgggttt
     1621 tgtcctgcct gaagcggctc attaacagga gtataatgat ggatttttct ttaactgaag
     1681 aacaagaact gctgctggcc agtattcgcg aactgattac gactaacttt ccggaagagt
     1741 atttccgcac ctgcgatcaa aacgggacat atccgcgtga gtttatgcgg gcgctggcgg
     1801 ataacggtat ttccatgctt ggcgtgccgg aagaatttgg tggtatccct gcggattacg
     1861 tcacccaaat gctggcgctg atggaagtgt caaaatgcgg tgctccggca tttttgatca
     1921 ccaacggtca atgtattcac agtatgcgcc gtttcggttc tgcagagcag ctacgtaaaa
     1981 cggcagaaag caccctggaa acgggtgatc ccgcctatgc cctggcgttg acggaaccag
     2041 gtgctggctc agataacaac agtgccacta ccacttacac gcgtaaaaac ggcaaggtct
     2101 acatcaacgg acaaaaaacc tttattaccg gtgcgaaaga gtacccgtat atgctggtgt
     2161 tggcgcgcga tccgcaaccg aaagatccca aaaaagcctt caccctgtgg tgggtcgact
     2221 ccagtaagcc cggcattaag attaacccgc tgcataaaat cggctggcat atgctcagca
     2281 cctgcgaagt ctatctcgac aacgtggagg ttgaagagag cgacatggtg ggcgaagaag
     2341 gaatgggttt cctcaatgtg atgtacaact ttgagatgga gcgcctgatc aacgccgcgc
     2401 gcagcaccgg ctttgccgaa tgcgcctttg aagatgccgc ccgctatgcc aaccaacgta
     2461 tcgcttttgg taagcccatt ggtcataacc agatgatcca ggaaaaactg gcgctgatgg
     2521 cgattaagat cgacaacatg cgcaacatgg tgctgaaagt ggcatggcaa gccgatcagc
     2581 atcagtcact gcgcaccagc gcggcgctgg caaaactgta ttgcgcacgt accgcaatgg
     2641 aagtcattga tgatgcgatt caaatcatgg gcggtctggg ctataccgat gaagcgcgcg
     2701 tctcccgctt ctggcgtgat gtccgttgtg aacgtatcgg cggcggtaca gacgaaatta
     2761 tgatttacgt agcaggtcgg cagatcctga aagactatca gaacaaataa tctgcaggcg
     2821 gcgcagcttc ttaacaaact gcgccgccag atttatccaa caagacttac cggttgagga
     2881 aattcagata ggatttcttg ttccgcaaaa cagtttttaa attgtctacg gtaatcgctg
     2941 ggcgaacaac cgacatggcg caaaaacagt ttggcaaagt gatcgacatt ttcataaccc
     3001 acacgccagg agatctctgc ctgtgataat tcagtattag tgagtgacca ttttgcttcc
     3061 gtcatacgcc gttgaataac atagttaata ggggaa
//
LOCUS       DAZFRN010000296         3091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFRN010000296 DAZFRN010000000
VERSION     DAZFRN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.299"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1170
                     /locus_tag="ACHKUB_005063"
     CDS             <1..1170
                     /locus_tag="ACHKUB_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6657933.1"
                     /translation="GNHAAQFGALVDKFRADLADMAGQCVGGSAGGVPWICGDTTYFW
                     KQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKW
                     RDSSATWTSQDRASHFSTWARRGIISDRLATAILIHAGRTTVKVDAPSSETGAPTPSP
                     SENEAVSTTTLLSYRASESEGRLTEQGWSAGGGKAEIVADEGATGGTAMKLSKETGVG
                     SWYLEHDAGTGAELLKNGGLISCRFKVSGELVANQYVIALYWPVSSLPQGVTLTGDAG
                     NNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGSFGAFDNEWHTLAFRFAGNNSLQVT
                     PVIDGQDGTPFTLTQSPVNAFAADKLHVTDITKSATYPVLIDSIAVEVNNADAAA"
     gene            1306..1488
                     /locus_tag="ACHKUB_005064"
     CDS             1306..1488
                     /locus_tag="ACHKUB_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6657934.1"
                     /translation="MTFIHQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SASQSAGSKPEEPITP"
     gene            1514..1762
                     /locus_tag="ACHKUB_005065"
     CDS             1514..1762
                     /locus_tag="ACHKUB_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6657935.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGEPTPDSDMMMM"
     gene            1839..2054
                     /locus_tag="ACHKUB_005066"
     CDS             1839..2054
                     /locus_tag="ACHKUB_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6657936.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            2059..2592
                     /locus_tag="ACHKUB_005067"
     CDS             2059..2592
                     /locus_tag="ACHKUB_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6657937.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIQVPLTEPQK
                     AGIASFCPYNIGPGKCLPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            2607..3056
                     /locus_tag="ACHKUB_005068"
     CDS             2607..3056
                     /locus_tag="ACHKUB_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6657938.1"
                     /translation="MIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKARELEQANAT
                     ITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGTVREATG
                     TSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCLN"
BASE COUNT          780 a          730 c          910 g          671 t
ORIGIN      
        1 ggaaatcacg cagcacagtt tggtgcgctg gttgataaat tccgtgcaga cctggcggat
       61 atggcaggcc agtgcgtcgg tggctctgct ggcggtgttc cctggatatg tggagatacg
      121 acgtatttct ggaagcagaa gaacgaatcc tcgtaccaga cggtgtacgg cagctataaa
      181 aacaaaacgg aaaagaatat ccatttcgta ccgttcatga cggatgagaa cggggtgaat
      241 gtgccgacga acaaaccgga agaagacccg gacattccgg gtatcgggta ttacggttcg
      301 aaatggcgtg acagctcagc cacctggacg tcacaggaca gggcgagcca tttcagcacc
      361 tgggcacgcc gggggattat ttccgaccgt ctggcaacgg cgattctgat tcatgcagga
      421 cgaaccacag taaaagtcga tgctccatct tctgaaactg gcgcaccgac gccatcaccg
      481 tcagaaaatg aagcggtaag cacaacaaca ctgctgtctt accgtgccag tgagtctgaa
      541 gggaggctga ccgaacaggg ctggagtgct ggcggaggta aagcggaaat tgttgccgat
      601 gaaggtgcaa caggtggtac cgcgatgaag ctgagcaagg agacaggagt ggggtcatgg
      661 tatctggagc atgatgccgg tactggcgct gaactgctga aaaatggcgg tttaatcagc
      721 tgccgcttta aggtatccgg tgaactggta gcaaatcagt atgtcattgc actttactgg
      781 ccggtttcct ctctgccgca gggtgtcacc ctgacagggg atgcagggaa taatctgctg
      841 gcagcgttct acatccagac ggatgcaaaa gacctgaatg tgatgtacca caatgcgaaa
      901 gtggcgacaa acaacctgaa actgggaagc tttggcgcat ttgataacga atggcatacg
      961 ctggccttcc gctttgccgg gaataacagc ctgcaggtga cgccggttat tgatggtcag
     1021 gatggtacac cgttcacgct gacgcagtca cctgtcaatg catttgcggc ggataaactg
     1081 catgtgacag acattaccaa aagtgcgact tacccggtgc tgattgacag cattgcggtg
     1141 gaagtgaaca acgcggatgc cgcggcatga taaaaaagcc gccagttgcc tgagacaacc
     1201 ggcggtgtga gattcatgga gaatcaagga aagataccat cactttcgtc actggcattt
     1261 ttaaacgaaa actgtttact tagtcaacca taacggtaag aaactatgac gtttattcat
     1321 cagctgatgc tgtacttctg tacggtggtc tgtgtgctgt atcttctttc gggtgggtac
     1381 agggcagtgc gcgattgctg gcgcaggcag attgataaaa gggccgcaga gaaaatcagc
     1441 gccagtcagt cagccggaag caaacccgaa gagcccatta ctccttaata acccctttca
     1501 acgagaaaat cccatgtcag aaattacatc cctggtcact gcagaggcag tgaaggaagt
     1561 cctgcgctct gaagaagtcc tgagcgcact gaaacagaaa cttcgccata acctggaagc
     1621 gcgtcttgat gcagaagtgg atgccattct ggatgaactg ctgggcgcac cggcagctcc
     1681 ggagccggaa ggcatcgcgg gtgaggggag tgcttcagat agcggtgaac ccacacctga
     1741 cagcgacatg atgatgatgt aagcatgcgc aagggaccat cggtgtgtgc cggtggtctt
     1801 tatattgttg tgagcttccg gattgcggga gacggggtat gtaccagatg gaaaaaatca
     1861 caacaggtgt gtcatacacc acgtcagcgg tgggaacggg ctactggttc ctgcagttgc
     1921 tggacagggt ttccccgtct cagtgggcgg caataggcgt gctggggagt ctgctgtttg
     1981 ggctgctgac atatctgacg aacctgtatt tcaagattaa agaagaccgt cgtaaggcgg
     2041 cgcggggaga gtaagctgat gagcaggaaa ttccgctatg gtttatcagc ggtcgttctg
     2101 gcgctgattg ctgcaggtgc ttctgcgcct gaaatcctcg accagtttct tgacgaaaaa
     2161 gaaggtaacc acaccacggc ataccgtgat ggtgcgggta tctggaccat ctgccgtggt
     2221 gccacccggg tggatggtaa gcctgtcgtc cccggcatga agttgtcgaa ggaaaaatgc
     2281 gaccaggtta acgccattga gcgtgataag gcgctggcat gggtggagaa aaacatccaa
     2341 gtgccactga ctgaacccca gaaagcgggt attgcgtcat tctgcccgta taacattggc
     2401 cccggtaagt gtctcccgtc gacgttttat aagcggctta atgccggtga tcgtaaaggt
     2461 gcctgtgagt cgattcgctg gtggattaag gacggtgggc gtgattgccg cacacgttca
     2521 aataactgct acggacaggt tattcgtcgt gaccaggaaa gcgcattagc ttgttggggg
     2581 atagatcagt gagaagagta accgcgatta tttacgctct ggttatctgc atcatcgtct
     2641 gtctgttatg ggctgttaat cattaccgcg ataacgccat cacctacaaa gcgcagcgcg
     2701 ataaaaaagc cagagagctg gaacaggcga atgccaccat tactgacatg caggtgcgcc
     2761 agcgtgatgt tgctgcgctc gatgcaaaat actcgaggga attagccgat gcgagagctg
     2821 aaaatgaaac tcttcgcgct gacgttgccg ctggtcgtaa gcgcctgcgg atcaacgcca
     2881 cctgccacgg taccgtgcgt gaagccaccg gcacctccgg cgtggataat gcaaccggcc
     2941 cccgactggc agacaccgct gaacgggatt atttcatcct cagagaacgg ttgatgacaa
     3001 tgcagaagca gctggaaggg gcgcaggaat atatccgcac tcagtgcctg aactaagttt
     3061 tgctgatgcg ccgtatcgtc gctgtattcc c
//
LOCUS       DAZFRN010000297         3068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFRN010000297 DAZFRN010000000
VERSION     DAZFRN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.300"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            75..1565
                     /locus_tag="ACHKUB_005069"
     CDS             75..1565
                     /locus_tag="ACHKUB_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO6657939.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(1687..2259)
                     /locus_tag="ACHKUB_005070"
     CDS             complement(1687..2259)
                     /locus_tag="ACHKUB_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657940.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2345..2551)
                     /locus_tag="ACHKUB_005071"
     CDS             complement(2345..2551)
                     /locus_tag="ACHKUB_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6657941.1"
                     /translation="MNTPVSLMADQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
BASE COUNT          806 a          672 c          795 g          795 t
ORIGIN      
        1 ttgatttgac tgctattttt gctattaata gccagaattg cagtgctttt atatctgtgt
       61 atagtgaggt tactatgtca ttcggtcagc cttgtgatga gtttcccctg agttcgctgc
      121 ctccactcat tcgtgatgcc gttattgaag ctcagcaaat tacgcaggct ccccttgggc
      181 ttgtggcagc gtctgccctg ggcgctgttt cacttgtctg ccagaatctg attgatgtct
      241 gcagactgaa tacactgcgg gggccggtct ctcttttttt cctgacgctt gccgagtccg
      301 gagaacgaaa aaccgcagtg gacaaattat taatgaagcc cctgtaccag caggagatgc
      361 agttatattc cagatacaaa agtgaactgg ctgtctggaa aaacaaggaa gagcttttaa
      421 aggctcagaa aaaagcattg ttgtcaaaac tgaataaaga actccgcaaa ggcgcggatg
      481 agtcagaaac cctcagacaa ctggaagtac ttcagaaaaa cagtgcggaa gaacctgtga
      541 ggtataaatt catttttaat gatgccacga ctgcggcaat taagaatcag ctttgtggta
      601 aatggcgttc agttggtatt atgtcagatg aagccggtat tatatttgat ggatatacac
      661 tgtctgagtt gccttttatt aacaaaatgt gggatggctc tgtattgtct gtggacagaa
      721 agaatgaacc cgaacagatg attgaaaatg ccagaatgac gttatccctc atggtgcaac
      781 ccggtctttt tgaccgatat atggagcgaa aaggttctgt tgccagggac agtggttttc
      841 ttgcccgttg tcttatcagt aaacctgcaa ccacgcaggg gaaacgcttt attaacggag
      901 ctgtgattcc gggaggatca ttaacggcat ttcatgaacg attaatggaa ctggccagag
      961 gcagtattga aaaatccagt gaggatgaac ggtattgtct gcatttttcg cctgaagcgc
     1021 aaaaaatatt tattgagcac tataatgttc tggagcagga tttatcccct tccggaccct
     1081 taagcccgtt tcggggacat gtttcaaaaa aaacagaaaa cattgcaaga attgcagcgt
     1141 tatttcagta tttcagctat ggtgaaggga aaatctctgc cgatatcatg acgtctgctg
     1201 tcgtaataag ttcgtggtac acagatgaat ataaaaaatt atttgcgtta cctgatgaat
     1261 ctgaactgca gctgcaggac gcccgggagc tgctcgactg gctcattgag gagtgccgcg
     1321 gagaatgccc gccccgtgtc aggaaaaatt atattctcca gtgtggcccg ggccggttca
     1381 ggaacaggaa aaagctgaac gtcctgctga acattcttgc atctcaactc aggctctctg
     1441 tcgtgcagga aggaaaaacc acctatgtcc tgctttccga aataaacaat atcacgctgc
     1501 ctgagctgaa tgcgtgctac agccagaaac tgataagatg gcatgagcaa tcctttggct
     1561 tataatgttc cctgttgctg agacggatta ttcagtggtg gtatcgtagt atgcccgtca
     1621 ccccccgctt ctgcatgagg gtgacggagg aaaagtaagt gtgcggaggc ccgggagcgt
     1681 tatgaatcaa caggcgtata acaggtactc gtcaatcatc agttccagct cgcgcttttc
     1741 ccgtttgcgc gccacagccc agttttcaag ctctgcatgt gatttatgct ttaactcttc
     1801 agtccagggc tgcatggagt gggcacacat cagatcatac cagcacagcc atatcagcgt
     1861 gtggtagaga tcacgatcgt cgaaacgccg gagcaggctg ttcagcctgt ttgccagccg
     1921 gttttcaccc agacgatgta gctcactgag taaaacgccg cccagattaa aatgtgtaag
     1981 cagcatgatt tcatgggtct gaatgctggt cattaaccct tcctgaatgg caggaatgaa
     2041 cggtggtgtt cccgggttga cgacctgata gagtccctgt ggtgccactg agatgatagt
     2101 gcctttttct ggcagaaaaa agtgtgccag ggtcaggcgg tgataaccat gaagatgcgg
     2161 agaccccatt ccatgagcga cgataagcct cgtcacgctt gtcatccatt tctgatgctg
     2221 gatgagcagc agctcacacc ggttttcaag tttttgcata atattctctc ctttagcgcg
     2281 cagcaaaatg cctccggggg ccggatcatg cctttgctgc ggttgaacgg ataacggtca
     2341 ggaattacgg acgggactgt gcaatacgcg cctgcagcca ggtttccact tcacttttca
     2401 gccagcggga actgcggccc agtttgatgg gggcaggaaa ggcaccatcc ttgatgagct
     2461 tgtaaaacca tttatcggtc aggccggtca gttgagtgat aaatgccatg tcgaccatct
     2521 ggtcagccat cagtgaaact ggggtattca tgaaaatgtt cctccggatg ttgttaatct
     2581 caaccggcgt tggtgatgaa aacaacgcac caccttcacc ggcataacaa ccggggagaa
     2641 acgtaaaaaa taatcgggaa tgtgaccgtc aggagataaa cggggggatt gcggtggctg
     2701 agccgtaccg gtatgtcggg tgattgttgc gaaacgatgc ttccgcacgc gcacgggagg
     2761 actttcgtga gacaagggga aaactggcgt tctgataatt gcagagttgt ttttgaaggc
     2821 ggttgtggtg catcagcctg tgacctgtgg gcaggcgaca ggtatcactg gtgggataag
     2881 actgtctgat tggtattata aatatataag agagctcaaa cacatgagtt catatatgtg
     2941 aaataaatct gtgagtgtga tcagcagacc agtctaccca gtagcaacag attgcgttgt
     3001 actcacaggg ttatccctga taactggcgt ataacctgcg tacataaaac gtacctgcaa
     3061 tgcacctg
//
LOCUS       DAZFRN010000298         3049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFRN010000298 DAZFRN010000000
VERSION     DAZFRN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.301"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..309
                     /locus_tag="ACHKUB_005072"
     CDS             <1..309
                     /locus_tag="ACHKUB_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657942.1"
                     /translation="PSGGTPVKAAVGNAVILLAKDVIYDEYFFDLRQSFFVSVKDLMA
                     SGAKGIPETQKYPDVYCWIPLFSINSGVVITPVYKIDPRKPVTVKKPDQITVVCNYRE
                     "
     gene            693..899
                     /locus_tag="ACHKUB_005073"
     CDS             693..899
                     /locus_tag="ACHKUB_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6657943.1"
                     /translation="MTTPVSLMDDQMVDMTFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            987..1595
                     /locus_tag="ACHKUB_005074"
     CDS             987..1595
                     /locus_tag="ACHKUB_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657944.1"
                     /translation="MKMKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIHYFQN
                     LTPGWVSFNGEKPKIAIVPRSLHRLIYGSDKRATPPLDDDLVVNLCTSEHLLVHHPML
                     EGILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTENLK
                     RKSEKELGEWIIDRQKQNAALTDLMDQYVLLAYRTTVDGNRN"
     gene            complement(1498..1698)
                     /locus_tag="ACHKUB_005075"
     CDS             complement(1498..1698)
                     /locus_tag="ACHKUB_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657945.1"
                     /translation="MAQYDDDEHALQVSMQGRGYHHFRILPHLPETTGISYGYRQPLC
                     GMPGARTDPSDPSVLRSASDGR"
     gene            2058..2210
                     /locus_tag="ACHKUB_005076"
                     /pseudo
     CDS             2058..2210
                     /locus_tag="ACHKUB_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          808 a          772 c          758 g          711 t
ORIGIN      
        1 ccatccggtg ggacacctgt aaaagcggct gttggtaatg cagtcatcct gctggcaaaa
       61 gacgtgatat atgacgagta cttttttgat ctgcggcagt catttttcgt gagcgttaaa
      121 gatctgatgg cctctggcgc aaaaggtatt ccggagacac agaaatatcc ggacgtttat
      181 tgctggatcc cgcttttctc cataaattcc ggcgtggtca ttaccccggt ctataaaatt
      241 gatccccgga aacccgtaac ggtaaaaaaa ccggatcaga tcactgttgt ttgtaattac
      301 agagaataat ctcagtcttc tccgctcacc gcaacagtgt ttacctcgct ttatcttccg
      361 ttcagtaaat acaaacctcc gctctggcag gtacagggcg tggtttatct ccggtactcc
      421 cctgcacagt aataatcatc actgaaaata ctgatcccgt cacggcaaaa cgccatccca
      481 tattccggca acacaaaatc atcttccgtt tataccggct tcactgacaa aacagacaac
      541 ctgtattgcc tgtacacaac agtttctctc ctggcaggaa tattccccta ctgatttttt
      601 acgtttctcc ccggttgtta tgccggtgaa ggtggtgcgt tgttttcatc atcaacaccg
      661 gctgagatta acaacatccg gaggagattt ttatgaccac cccagtttcg ctgatggatg
      721 accagatggt cgatatgacg tttatcactc agctgaccgg cctgaccgat aagtggtttt
      781 acaagctcat caaggatggg gcctttccgg cacccatcaa actcggccgc agctcccgct
      841 ggctgaaaag tgaagtggaa gcctggctgc aggcgcgtat tgcacagtcc cgtccgtaat
      901 ttttaaccac tggcccttcg cccacagcag acgcaatctc cggcctgccg gcggcactct
      961 gctgcctgtt atcccccgtg aggaatatga aaatgaaacc acagtatcag acccgttacg
     1021 agctgctcca cgaaagctac cagaaatggc tgaccggctt cacccggcac gccgtatcct
     1081 ggggcgtgtg tcatccgaat atccactact tccagaatct gacgcccggg tgggtgtcat
     1141 tcaatggtga aaaaccgaag attgccatcg taccccggag tctgcaccgg ctgatttatg
     1201 gctctgacaa acgggccacg ccgcccctgg atgatgatct ggtggtgaac ttatgcacca
     1261 gcgaacatct gctggttcat catccgatgc tggaagggat tctgctgtct gaatgtgaac
     1321 gtctgaggca gcgttctctg gcaaataaac tgatcagcct cttccgtcag tttggtggca
     1381 cggagttgcg cctcaaactg gtctggcttt gctggcttga tttaatgacc ggaaacagcc
     1441 ttgacgactg gacggagaac ctgaaacgga aatcagaaaa agagctgggg gaatggatca
     1501 tcgaccgtca gaagcagaac gcagcactga cggatctgat ggatcagtac gtgctcctgg
     1561 cataccgcac aacggttgac ggtaaccgta actgatgccg gttgtttccg gaaggtgcgg
     1621 caaaatacgg aaatgatgat acccccggcc ctgcatgctc acctgcagag cgtgttcgtc
     1681 gtcatcatac tgagccatga cggatgtctt aagggcatcc tgtcattgct gagctggcat
     1741 gctgcggtac atgcctcacc ctggtgacaa taagtacatg gcgacagcac tccctgcacc
     1801 gcaaaagaga tgtacacggc cggatgttat ggctgaatgt ttcttcgtca gatattcagg
     1861 aacagttgta gtaacgaatt ttaccgatct ctgtacacgg ccggacgccg tcagtgtata
     1921 cgggcggacg ttccatgctg catgactaca tccggcccgg atgtcaatga gtccgtcagg
     1981 aacgcttcgc ttcgaaaccc ggccaggata ccagcgccgg cacgccggga agaagttgat
     2041 atggtggttg ccggacaaca gcgctcctgg attgactgct gcatcactca accacgctga
     2101 atatcaaagg agagcgttac cgattaaaag agaaacggaa agctgtggtg atgacccaaa
     2161 acaacacgac aatcagtgag gatggaatgg tgaaaatcag actgcatcag tgtagaaaat
     2221 atactggctg gcatgaaaat ggcccggctc gtggcgttga catattctct cctgagcatc
     2281 ctgaatgact gcagttgctg tggaaaagta agcagtctcc acccacggca ccacttaaca
     2341 gacattcagt aactcacccc agactttcta taacgcttat cggtgcactg aaaacaggag
     2401 agtgcgctta aaaccgcaga ccgcattgcg ggtgttactg catgacagcc tggcggtatt
     2461 gtgaaatgtc ggcaggaacc gatgtctgcg cagggggaac ttccgtgatc gggataagtg
     2521 cagaagaaat cagatcacga cgtaaacctg cagtgcctga cgctgcaagt ggcattaacc
     2581 atgggacgtt gccgatcccg gattgcggaa acaggagcac tattttctgc cgcggggagt
     2641 acctctggca gtaaatccgg ctcgctcatc tgaccgtctc tctcccggaa aagaccagaa
     2701 atgagatcgt cacctcaaca gcagggaaga tgtcttgaca gactggttat attgcatacg
     2761 atttatgaag aaaaagacag caaatatgga ggctatagcg gagaacaccc tacgaaattc
     2821 ctgtgaaaaa cgattgaaaa aacgatcaga ttcgattcgt ttttatatgc tttttgggca
     2881 cgtggcgatg acgaactgtt gcatgtgcag aggagaatgc acccggaagc accagaatca
     2941 tccagaaagg cagaacgatt acgcatcaaa ccaggagaca gcaatatcag gataacatac
     3001 tgatttaaaa tgaatttaca gcaatacgtg aaaacatttt ttacttaaa
//
LOCUS       DAZFRN010000299         3010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFRN010000299 DAZFRN010000000
VERSION     DAZFRN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.302"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            302..415
                     /locus_tag="ACHKUB_005077"
     CDS             302..415
                     /locus_tag="ACHKUB_005077"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657946.1"
                     /translation="MTVVLKSLSEELTTREAAARFNISNESVIRHWVKKVF"
     gene            complement(522..2068)
                     /locus_tag="ACHKUB_005078"
                     /pseudo
     CDS             complement(522..2068)
                     /locus_tag="ACHKUB_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612334.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2432..2654)
                     /locus_tag="ACHKUB_005079"
                     /pseudo
     CDS             complement(2432..2654)
                     /locus_tag="ACHKUB_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846847.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          805 a          730 c          704 g          771 t
ORIGIN      
        1 tagcgaccaa ggcggtagtg attaaagggg gggggatata aaaatttatt gtacgtggac
       61 tgcaccccaa aacttggaga cccaactgat taatgtgcaa tttttatggc aaagccaaaa
      121 tattctctcg aaaccagatt ggttgtagtt aatcattatc tttccagaca cgatggcgca
      181 cgtcgtatcg ctaaacgttt tggtgttgaa aaaacatctg ctgggtaagg taagagactg
      241 gtaactccac gatattgatc gtatcacatg gaaaaatgac ctccacactc ctgagtttcg
      301 actgacggtt gtcctgaagt ctctgagtga agaacttacc acgcgcgagg ccgctgcacg
      361 gtttaatatc tcaaatgaat ccgttatccg gcactgggtg aagaaggtct tctgaacata
      421 aaaccaggcc ggagcaaaga catgacaaaa tctcaaaaaa cacctccact tactgatgca
      481 gaactggaaa agtggctctt ctgcaacctg ggtggagatt ctcatgccag tggactcatt
      541 accctctgct tgagtagttc aaatccggct cgaccataca tctggcgttt caacatcttc
      601 aggcgattta catgaccttc gacaacacca ttactccagc gactgctgat tgcctcacat
      661 atagccgcag catcagcttc catccccgca gccacgcacc gaagttctgc tgagccgctt
      721 tcgtggactc gagtgaacaa gctgcttagc tgtgatttat tttgggtttt cagaatacgg
      781 tagaactcga gtaccagttg ctgcgctatt ttcagctccg gttctttttc acacatcaga
      841 ctaataaatc gggaagcata attttcttca cccctgatta ttaaccagtg gcttacccgg
      901 gacactgaag gaagtctgaa ggaagtctga cggcagtagt aaccggtggg atccagcctt
      961 tacgcgattt ggcaacagca tccctgacga tggtttcact gcctgtaccc ccctggccac
     1021 catttcccgc catatcctgc tggcgttata attaccactt tctcgttgct cttttaacca
     1081 ctccctccat gggtccagaa gccctcgctt tggaggccgg gttgacattt cagggaatgt
     1141 tcctgaacgc cccctgcgac tgactgtcac acgcgacagg cctgtaatac gggatatttc
     1201 cctgaaacta catcccttgt tatgcagggc cataacctct gtccaaagct gatgccgttt
     1261 tttgcgggtt tgctgtttaa ggcgttccgg acgacggagc gatactgcag gcacagatgg
     1321 ttttgccccg ggttattcct ttggtgacaa ctaactggta acaagacgta tcagagtcat
     1381 atgtctgtac atcattcgtt caggcgcatc gccaatattg tttagcagaa gccatcgttc
     1441 agctacctgt ctggcctgag gtgcaccttc acgtgctgct gtcgcattga ttccgcagcg
     1501 atcacgcgag acaacctgta tttccggata ttttctgaac caggtcgcca gcgtatgctg
     1561 atcgcgaccg tgcaacagga cgaggggacg gtgagtatca agattgacta ttaacatacc
     1621 gcagcagtgg ccccgatgcc acgccactca tcaataccga catggggcga gcctgacagt
     1681 ttcgtctcca gggtattata agcctgcgaa gaagagtatc tgcactgcaa cggagaccca
     1741 ctgtcgttgc tgcccgtttt ccagcctcac ctccggctat taatcccatc tgacgctgta
     1801 aattttgtaa cgactgtgaa gactgctggt gtgaactggc taagggagca agcgacccgg
     1861 caaaaatttt acgtgaacaa gccgggttac ggcagtacta gtggcggacg gaaaatacaa
     1921 gccatagcgt ctggcctgtg cagggtagat gctgtatccg gcgccgggga caactatgaa
     1981 cagaatcgct acgcttaaag cattcaggac attgcgctgt tttgcgtttt ccgcggagat
     2041 ggagggttat accgtcagat ctaataattt gtcgactgca ttaccatccg caaggtaact
     2101 gtagaagtgt cttaagagag tgcattgcgt tgcccataac tgaaaacagc ttagatcctt
     2161 gttcaccaga aagtttcatc cattttcttc cacccaggtt gcggaagagc tacttttcat
     2221 ccgtcgttga caaataatga tgagatagtc acagagcagc gtttgggaag cgcacaatca
     2281 caatattgta cggttgcatt atcgcggtag agtataccgc atgctaaaaa taagttaagt
     2341 aagcgtctga atgatattac atactatatg tatggagcca aaatacattt ttgattaatt
     2401 acttgctttt tcaggacgga gacgtatatc tcatcctgga tacaactcca cagagaggag
     2461 aattgttttt atccggaaca aatctaggac cacgcaggaa acagagtaat accgcaaagt
     2521 tctagtgatt agctggacac cgctgcttat taatgagagc aatatttctg taacaaagat
     2581 aacacattct gagtcagaat aatttcatca tcaggtataa ccagcaggca agcttgcgaa
     2641 cggagcgtgc acacagccca gcttgcagcg aacgacctgt accaaaatga gataccaaca
     2701 gcgtgagcta tgagaaagcg ctacgcttcc cgaagggaga aaggcggaga ggtacgcaca
     2761 agggagcttc cagagggaaa gggagtacca gctggcgaat ttgcactcca acaaataatt
     2821 cccccttttc tactccacta cttttgacca tacgtctggc gcttaattcg ttgattgttg
     2881 gccttgcaag cgccgttaga cctgattcac catacccagg cagtcttgga aacggaaagc
     2941 ttgcgcgtta agcgcacgtt ttaactaatt caaatacata atgaattgat tcaggaataa
     3001 attgtgatgc
//
LOCUS       DAZFRN010000300         3000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFRN010000300 DAZFRN010000000
VERSION     DAZFRN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.303"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="ACHKUB_005080"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="ACHKUB_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO6657947.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            512..838
                     /gene="hdeB"
                     /locus_tag="ACHKUB_005081"
     CDS             512..838
                     /gene="hdeB"
                     /locus_tag="ACHKUB_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO6657948.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            902..1549
                     /locus_tag="ACHKUB_005082"
     CDS             902..1549
                     /locus_tag="ACHKUB_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO6657949.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLLNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1591..2121)
                     /locus_tag="ACHKUB_005083"
     CDS             complement(1591..2121)
                     /locus_tag="ACHKUB_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO6657950.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2277..2843)
                     /gene="slp"
                     /locus_tag="ACHKUB_005084"
     CDS             complement(2277..2843)
                     /gene="slp"
                     /locus_tag="ACHKUB_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO6657951.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          866 a          597 c          664 g          873 t
ORIGIN      
        1 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtaagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
      481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
      541 tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg cgctggcagc
      601 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
      661 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgataccgt
      721 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa
      781 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
      841 aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt tgcattttta
      901 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
      961 catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat taattggcat
     1021 gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga
     1081 tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     1141 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga cggcagcgga
     1201 tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg aactgggtat
     1261 ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa ccttccgcct
     1321 gatgaacaaa aattaccatt tacagctgac attactgaac ggtaatacgg tatcaatgct
     1381 cgactggttt aagcagcaaa aaataaaaac ggatttagtt tcgttgcagg aaaatgaaga
     1441 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagacctgtt
     1501 gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat ctgtgtggca
     1561 gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat gctgatggcg
     1621 taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt tatggcagta
     1681 gaacgttttt agcgatattt tcagtttctc cagaatatct tccgttgtca tttgattcgc
     1741 aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acagccgctt caggcacaga
     1801 gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa
     1861 ggtcaccggc actttcccca ggcagcgttt aatattaaag ggggagaaaa taatcacatg
     1921 gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat tattcattaa
     1981 tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt cttcgttctg
     2041 tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga acatcgtatc
     2101 cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt ataatcagtt
     2161 tgccaaaggt aattaacagg acagggctta taataataat agttagctta ctgtatatta
     2221 attttatgta atacatgatg cagatgctga gaaagcatct gcatctttcg gtggtgttat
     2281 ttgaccagct caggtgttac ctgactcacc gcattggtgt agtaaggcgc accccagccc
     2341 ggttccggcc agaatgcacc atagccgtaa tcccacaggt tataggtggt attaaccact
     2401 tctctcaaat gccacacctg gatgccctgc atattcactt ccaggaagtt atacgggact
     2461 ttattgataa agcctggttg ttcaccctga atggtaccga ggatggtaac aaagtgatta
     2521 cgatagttca ctggatcaag gaagccgctt tgtctggcga gcagtcggcc ctgatagttg
     2581 gcttcaatat caggcttcgc atagctatcc aacggtaata cagcgatttc taacaacgta
     2641 tccgttttgc cattgataac gttgataacc ttcccaccaa agcgcgcttg ttgaccaaca
     2701 tataaccccg gctggttatg aacagcaaca aaactttttt gaatatcagg ttggttattg
     2761 cctttgatat tttgcggaat tgaactacat gcggcaagca aaaatgaaag gctgaggatg
     2821 agtgcacctt ttgtcatgtt catgttacta tccttatcaa caaatattaa aaccataata
     2881 gatatttctt tatagttttc atctgattct gaggtgatat gcctgatagt aacgtaaacc
     2941 tgtctgatgt ttatatccct tctgtttagt tcaattccgt tattttgggg cgtggtatga
//
LOCUS       DAZFRN010000301         2933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFRN010000301 DAZFRN010000000
VERSION     DAZFRN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.304"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(125..2386)
                     /gene="katE"
                     /locus_tag="ACHKUB_005085"
     CDS             complement(125..2386)
                     /gene="katE"
                     /locus_tag="ACHKUB_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO6657952.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            2568..2831
                     /gene="cedA"
                     /locus_tag="ACHKUB_005086"
     CDS             2568..2831
                     /gene="cedA"
                     /locus_tag="ACHKUB_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO6657953.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          705 a          721 c          761 g          746 t
ORIGIN      
        1 aataaactgc aatacattga atttatacga ctttgtaggc cggataaggc gttcacgccg
       61 catccggcat aaacaaagcg cactatgtaa atcatttgag gcggcgcaat tgcgccgcct
      121 cccatcaggc aggaattttg tcaatcttag gaatgcgtga ccacacgcgg tgtgctgcca
      181 tcagcgttag cagttcatcc ataaaactac cgtcagcgct gtcagcttcc acaatccctt
      241 cttcaccctg gtcagcgacc ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg
      301 caatcggttt aaggtgtttg taggcttcca tcaggtagta gttggcatcg ccgttgtcag
      361 cgatatccgc gatattgccg caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac
      421 cggcaaaggt agcggctata ggcaacaccg taccgtcatc cgcagtcact tcacccattc
      481 gggagtagag cagtttggca tgaacgcctt tggccttcag ggccttgaga atggccagaa
      541 ggtctgccga tctcacttca tcattaagta aaatcgctac cacgcgacct ttcacatcac
      601 cgtcaggaat ggcgtacaaa cttaaggatg gatccttttt cagaccgttg acgtccggag
      661 gtggggtgat attcagctgg tcgtcagtca gttcgatacc gagatttttc gccaccgcct
      721 gggccagagt gagatcaata tgcgccagct ggtcaacaac gcgctcacga atatacggac
      781 gaacgacttt gcttaactca aaactgaaac catcgacaat atggcgctgc tcgaatggcg
      841 tctgacttag ccagaacaga cgcggatggg aataatattc gccaaacgat ggactgcgct
      901 cacgaacttt atttccttcc acgcgttcct gatatgattc aaaaccgccg cgtttcggtc
      961 ccggcggtgt ttcgcgcggc cagttatcgt tgatcgagtt cggttcgtaa ttcgccgggt
     1021 tagtgtcgat ccccatacga tgcatgccat cacgctggaa gttgtggtaa gggcaggtcg
     1081 gacggttaat cggaatctca tggaaattcg gcccaccaag acgactgatt tgtgtatcgg
     1141 tataggagaa caaacgtccc tgcaacagcg gatcgttggt gaagtccaga ccaggaacaa
     1201 tatgacctgg atggaatgct gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat
     1261 tgagcaccat tttgccgaca cgctgaacgg gcaccagttc ttccgggatc agtttggttg
     1321 gatcgagaag atcgaagtcg aacttgaatt catcttcttc aggaatcaac tggaagccca
     1381 gttcgtattc cggaaaatcg cctgcttcaa tggcttccca caactcgcgg cggtggaagt
     1441 ccgggtcacg tccggtgagt ttttgtgctt catcccaaac gagtgaggct ttacctgcca
     1501 gtggtttcca gtggaaacgt acaaacgttg ccttcccttc ggcattaatc aggcggaagg
     1561 tgtgaatacc gaagccttcc atggtgcggt aactgcgggg gatgccgcga tccgacatcg
     1621 cccacatcac gttgtgcaga gtttcaggtt gcagagaaac ataatcccag aaagtatcgt
     1681 gggcactttg cccttgtgga attgcccagt gcggttctgg ttttaccgca tgaacaaaat
     1741 cggggaattt atgcgcatcc tggataaaga agattggcgt gttattgcca acgaggtcaa
     1801 aaataccctc ttcagtatag aacttggtgg caaaaccacg gatatcacgc acggtatcgg
     1861 cagagccagc gccaccctga acggtagaga acctaacaaa tactggggtg attttgttcg
     1921 gatctgagag gaaatccgct ttggtgatat cgcttaagct tttatatggc tggaaataac
     1981 cgtgagctgc tgatccgcgt gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt
     2041 gggtgatttt ctcgcgcaga ataaaatctt ccagcagcgt tggaccacgg ttaccggcac
     2101 gcagtgagtt ttgatcatcg gcgatgcgca cgccctgatt agtggtcagc gcataatttt
     2161 cactgccttt gcgtacgtct tccagagaat taagtttttc gttacgtgta tcaggggctt
     2221 tcaggctccc aggggcggta ggttgtgcac ctggcggtgt tggttcagcc gctggacgat
     2281 gagatccgtc ctcaggtgcc agtgagtcca tccccggttt cgcttcgctg gaatcgtgta
     2341 gtggtgactg gtgctgatgt gggttctttt cgttatgttg cgacattgaa ctcgtctcct
     2401 taatttatta ctgaaagggc cgctgttttg gtcaaaaaac cctctaacta tagaccatca
     2461 gccagatccc gcttcggaga ctattctgga aacggtgtta tgaaatacac gcaaagtggg
     2521 acgtacaggg gctaaatcgg ctaaactaca gtttttctat ttttaatatg cgtttagtga
     2581 agccagtaat gaagaaaccg ctccgccagc aaaaccgcca gattattagc tatgtcccac
     2641 gcacggaacc cgcgccgcca gaacatgcga taaagatgga ttcgtttcgt gatgtctgga
     2701 tgctgcgtgg caaatatgtt gcgttcgtac tgatgggaga gtcatttctg cgctcaccgg
     2761 cgtttactgt gcctgaatcc gcccagcgtt gggcaaatca gatccgccag gaaggggaag
     2821 tgactgagta aagattacgt acctcccggt tttgccggga gttacatgac ctgaaattct
     2881 gtatttcatt gctacattca ttcaaaccac cagtaacaaa aagctgcctt tga
//
LOCUS       DAZFRN010000302         2925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFRN010000302 DAZFRN010000000
VERSION     DAZFRN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.305"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            92..469
                     /locus_tag="ACHKUB_005087"
     CDS             92..469
                     /locus_tag="ACHKUB_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFO6657954.1"
                     /translation="MATCTENSSGAACQKAMNERDAVGLALATGTVALLPGSAQVMWG
                     LGASANAGISYLADGTVDPANAAIAGWVNVFSMGNGLAGTVGWNAAGGALGNWIGNKD
                     PLSGAISNGIGSAAGYGIGKGIT"
     gene            506..697
                     /locus_tag="ACHKUB_005088"
     CDS             506..697
                     /locus_tag="ACHKUB_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HFO6657955.1"
                     /translation="MTNGWDPKYDPTWIKYAEVNRNDIIREGMKPSNIPGTAGDIGGS
                     IGSELGGKVTDKVSEELKK"
     gene            968..1210
                     /locus_tag="ACHKUB_005089"
     CDS             968..1210
                     /locus_tag="ACHKUB_005089"
                     /inference="COORDINATES: protein motif:HMM:NF044555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6862 domain-containing protein"
                     /protein_id="HFO6657956.1"
                     /translation="MSEKTELEIAKQTLKNSKDPAEREKVQQKYDALLEKDIASDKEV
                     IAACSNGNAGSSACASARLKVIASKEGYEDGPYKRL"
     gene            1272..2478
                     /locus_tag="ACHKUB_005090"
                     /pseudo
     CDS             1272..2478
                     /locus_tag="ACHKUB_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc39 family transposase"
BASE COUNT          832 a          587 c          775 g          731 t
ORIGIN      
        1 tcgtttttat cgttgatttg ttgttgagaa taatttactt ggcggtagtg aatatgctca
       61 tgctgcgtgg atacgccagc atggtatcga catggcgaca tgtacagaaa actcttccgg
      121 tgccgcctgc cagaaagcaa tgaatgagag ggatgctgta gggctggctt tggcaacagg
      181 tactgtggca ctactccccg gtagtgctca ggtaatgtgg ggacttggag caagtgcgaa
      241 tgctggtatc agttatctgg ctgatggtac tgtagatccg gcaaacgcag ccattgcagg
      301 ctgggtgaat gtgttcagca tgggtaatgg cctggcgggg actgtgggct ggaatgctgc
      361 aggtggtgcg ctgggtaact ggatcggcaa caaagatcct ctgtctggtg caatttcaaa
      421 tggtatcggt tctgctgctg ggtatggtat cggtaaaggt attacttagg ggacagataa
      481 gattacgaat gctgtaggaa aatgggtgac aaatggctgg gatcctaagt acgatccaac
      541 atggataaaa tatgctgaag taaatcgtaa cgatatcatt cgagaaggca tgaaaccgag
      601 caatattcct ggaactgctg gagatattgg cggttctata ggatccgagc taggtggaaa
      661 agtaacagat aaagtatctg aggaactcaa gaaatgaaat attcttttcg agtggtaata
      721 ggtaagttta ttatatgcag ttcaattctc ttattgtgtt ttattggcat gatcttagcg
      781 gccataataa ctaaactaac gtttgtagcg ttagcatggt ttcaaagcgg gatttttgaa
      841 gttttgtgga gcgatattgt ttatgctgct cagttgggtg gtctgggggg gggcatatta
      901 ggtttttgtt gggttttgtt atatcttttt agggcaaagg gctttagttg agaataacta
      961 cctgagcgtg tctgaaaaga cagaacttga gatagcaaaa cagacgctta aaaacagtaa
     1021 agatccggca gaacgtgaaa aggtacagca gaaatatgat gcattgcttg aaaaagatat
     1081 tgccagtgat aaggaggtta ttgctgcctg cagtaatggt aatgcgggga gttcagcgtg
     1141 tgctagcgca aggctgaaag ttattgcgtc gaaagaaggg tacgaagatg gcccttataa
     1201 gagactgtaa ttaaaattgt gtaattgcct gtttttgata tgttcactcc aacaacggag
     1261 acaggcaaac gatggacgaa aagaaactta aagcccttgc ggctgactgg ccaaaggcct
     1321 taaaaccgaa gccgacctca accagttttc acgcatgttg acaaagctta ccgtcgaaac
     1381 ggcgctcaat gcagaactga ctgaccatct cggtcatgag aaaaatgctc ctaaatcagg
     1441 ctctaatacc cgtaatggct actcgtcaaa aacggtattg tgcgatgatg gcgagatcga
     1501 acttaacacg ccgcgtgacc gtgaaaatac cttcgaaccg caactaatta agaagcatca
     1561 gacacgcatc acccagatgg acagccagat tttgtccctg tatgccaaag gcatgactac
     1621 gcgggaaatc gttgccacgt tcaaagaaat gtacgacgcc gatgtctcgc ccacgctgat
     1681 atccaaagtg accgatgcgg ttaaagaaca ggtctctgaa tggcaaaatc gaccgttgga
     1741 tgcactgtat cccattgttt atcttgactg tattgtggtt aaggttcgtc acggcgggag
     1801 tgtcattaac aaagcggtgt tcctcgcgct gggcatcaat accgacggtc agaaagagct
     1861 actgggtatg tggctggcag aaaatgaagg tgcaaagttc tggctgggtg tactgactga
     1921 gctgaaaaat cgtggccttc aggacatcct gattgcctgc gtggacggcc tgaaaggctt
     1981 cccggaagcg ataaatagcg tgtacccgca gacgcatatt cagctctgca tcatccatat
     2041 gtgcgcaaca gcctgaaatt cgtgtcgtgg aaggattaca aagccgtcac cagcgggctg
     2101 aaagcggtat atcaggcccc gacagaggaa gcggcgttga tggcgttgga taagtttgca
     2161 ggcgtctggg atgaaaaata cccgcaaatc agcaagagct ggcgcacaca ctgggaaaat
     2221 ctcaacacct tcttcggtta cccaccggat atccgcaagg ccatctacac gacgaatgcc
     2281 atcgaatcgc tgaacagcgt cattcggcag gcgataaaga agcgcaaggt gttcccaacg
     2341 gacgactcgg tgcgaaaagt gatttatctg gcgatacagt cggcgtcaaa aaaatggagt
     2401 atgccaatcc agaactggcg gctggcaatg agccgtttta ttatcgagtt tggtgaccgc
     2461 cttagcgagc acctttaata cggcggcagt tacacagaat tatttacagg gtcgactggg
     2521 aacatattca ggcagtgtat ctgaatccag aaaagaaaca gcataatgta attttattac
     2581 aaaaggcgac aattagcttg aaaaacgccg ttattgggtg ttctactacc atgaaaatac
     2641 gcatttttca caccgcacat tacgaaactc gcatcacgga gaatgaaagc tatcagacac
     2701 tggttgctat ttttgaccac agaaggtagt gctgtttacc gccagagctg tgctggcggg
     2761 gagcgaatcc gaaccatgtc gataattgtc accggccaga gaactatgtg gcatcaattt
     2821 tgctgaaaaa tactgcagca atcagaggac tgacgcccgg gatttttaaa caaattcagt
     2881 tttcatcgta gtatcacttt atcagccaag tcataaagtt cagct
//
LOCUS       DAZFRN010000303         2885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFRN010000303 DAZFRN010000000
VERSION     DAZFRN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.306"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            2..478
                     /gene="agaB"
                     /locus_tag="ACHKUB_005091"
     CDS             2..478
                     /gene="agaB"
                     /locus_tag="ACHKUB_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO6657957.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            517..1320
                     /gene="agaC"
                     /locus_tag="ACHKUB_005092"
     CDS             517..1320
                     /gene="agaC"
                     /locus_tag="ACHKUB_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO6657958.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1310..2101
                     /gene="agaD"
                     /locus_tag="ACHKUB_005093"
     CDS             1310..2101
                     /gene="agaD"
                     /locus_tag="ACHKUB_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO6657959.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2102..2857
                     /locus_tag="ACHKUB_005094"
     CDS             2102..2857
                     /locus_tag="ACHKUB_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO6657960.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          704 a          678 c          726 g          777 t
ORIGIN      
        1 aatgagcagt ccaaatattc tcttaacccg tattgataac cgtctggttc acggtcaggt
       61 tggcgtgacc tggacatcca ccatcggtgc gaatctgctg gtagtcgtgg atgatgttgt
      121 cgctaacgat gatattcaac agaaattaat gggtattacc gcagaaacct acggctttgg
      181 cattcgtttc tttactatcg aaaaaaccat taacgtcatc ggcaaagctg caccgcatca
      241 gaagatcttc ctgatttgcc gtacgccaca aacggtacgt aaattggtag aaggtggcat
      301 tgacctgaaa gatatcaacg tcggcaatat gcatttctcg gaagggaaaa agcaaatcag
      361 cagtaaagtt tatgtcgatg accaggatct cgcggactta cgttttatta aacaacgtgg
      421 cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa gaacaaatcc ctgactaaat
      481 ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc atgaaataac cctacttcag
      541 ggattatccc tggcggcgtt agttttcgtt ctggggattg atttttggct ggaagcctta
      601 tttttattcc gcccgataat cgtttgtacc ctaactggcg ctattctcgg tgatattcag
      661 actggcttaa ttaccggtgg tctgacagag ttggctttcg ccggattaac ccctgcaggt
      721 ggtgttcagc cgcccaaccc gattatggcg ggtctgatga ccaccgtcat tgcatggtct
      781 acgggcgttg atgccaaaac agcaattggt cttggcctgc cgtttagttt gttaatgcag
      841 tacgtcattc tgttcttcta ttccgctttc tcattattta tgaccaaagc cgataaatgc
      901 gcgaaagagg cggatacggc agcgttttcc cggcttaact ggacaacgat gctcatcgtc
      961 gcttcagcgt atgcggtgat tgctttcctc tgtacttacc tggcacaagg ggcgatgcag
     1021 gcgctggtga aagcgatgcc cgcctggttg acccacggct ttgaagtggc tggcggtatt
     1081 ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt tcaaagcgca atatatccct
     1141 tacctgatcg ccggtttcct gtttgtttgc tacatccagg tcagcaacct gttgccggtt
     1201 gccgtactgg gcgcaggctt tgcggtgtat gagtttttca atgcgaaatc ccggcagcaa
     1261 gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg actacagcaa tgggatctga
     1321 aatcagtaaa aaagatatca cccgtctggg ctttcgttcg tcgctgctgc aagcgagctt
     1381 taactatgaa aggatgcagg cgggcggttt tacctgggcg atgttgccga tcctgaaaaa
     1441 gatttataag gacgacaaac cgggcttaag cgcggcgatg aaagataacc tcgaatttat
     1501 taatacccac ccgaatctgg tcggattcct gatggggtta ttaatttcga tggaagaaaa
     1561 aggagaaaac cgcgacacca ttaaaggcct caaagtggca ctgtttggcc caatcgccgg
     1621 gattggcgat gcgattttct ggtttacttt gttgccgatt atggcgggaa tttgctcatc
     1681 atttgccagc cagggaaacc tgctggggcc gattctattt ttcgccgttt acctgcttat
     1741 ctttttcctg cgcgtcggct ggacccacgt cggttattca gtcggcgtga aggcgatcga
     1801 taaagtgcga gagaactcgc agatgattgc tcgctcggca accatcctcg ggatcacggt
     1861 aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg gtgacatcgt ttgccatcga
     1921 cagtacccac agcgtcgcgc tgcaacagga tttcttcgat aaagtcttcc cgaacatttt
     1981 accgatggcc tacaccctgc tgatgtatta cttcctgcgg gtgaaaaaag cgcatccggt
     2041 gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt tccgcattcg gcattttgta
     2101 aatggaacga ggcactgctt ctggcggtgc ctctttactt aaggaatttc atcctgtgca
     2161 aacccttcag caagttgaaa actatacggc gttaagtgaa cgagccagcg aatatttatt
     2221 ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg gcgaccggag ccacgccatt
     2281 actgacgtat cattatctgg tagaaaaaat ccaccagcag caggttgata tcagccagct
     2341 caccttcgtg aagctcgacg aatgggtgga tctgccatta acgatgcctg gcacctgcga
     2401 aactttccta caacagcata tcgtgcagcc gctggggctg cgtgaagacc agctcatcag
     2461 ctttcgctcc gaagagataa atgagacaga gtgcgaacgg gtaacgaacc tgattgcgcg
     2521 caaaggcggt ctggatttat gcgttctagg attggggaaa aacggtcatc ttgggctgaa
     2581 cgaaccggga gaaagcctac aaccggcctg ccatatcagt caacttgatg ccagaacaca
     2641 gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact cgtgggatca ccttaggcct
     2701 gaaggatatt ctcaatgccc gcgaggtttt gttattggtg actggcgaag gaaagcagga
     2761 tgcgacagaa cgttttctca cggctaaagt ctccaccgct atcccggctt cgtttttatg
     2821 gctgcacagt aactttattt gtttaattaa tacctaacca gatgaaaatt tgctcaaaat
     2881 tttta
//
LOCUS       DAZFRN010000304         2883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFRN010000304 DAZFRN010000000
VERSION     DAZFRN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.307"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            115..747
                     /gene="comR"
                     /locus_tag="ACHKUB_005095"
     CDS             115..747
                     /gene="comR"
                     /locus_tag="ACHKUB_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO6657961.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(809..1348)
                     /gene="ycfJ"
                     /locus_tag="ACHKUB_005096"
     CDS             complement(809..1348)
                     /gene="ycfJ"
                     /locus_tag="ACHKUB_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6657962.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1575..2879)
                     /gene="ndh"
                     /locus_tag="ACHKUB_005097"
     CDS             complement(1575..2879)
                     /gene="ndh"
                     /locus_tag="ACHKUB_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO6657963.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          696 a          783 c          705 g          699 t
ORIGIN      
        1 ctttaaattt cagttaaata gaaagcaatt atttttatgg gatctggact ggtgaacgga
       61 ggtgattatt tttataataa cctttcattc acagtcacca ggtacaacgg catcatggca
      121 actgactcaa cacaatgtgt aaaaaaaagc cgtggccgcc caaaagtgtt cgacagggat
      181 gccgcgcttg ataaggccat gaaattgttc tggcaacacg gttatgaagc gacttctctt
      241 gcggacctcg tcgaagcgac cggagccaaa gcgcccacgc tatacgcgga atttaccaac
      301 aaagaggggt tatttcgtgc cgttctcgac cgctatatcg atcgttttgc cgctaagcat
      361 gaagcacagc tgttttgtga agagaaaagc gtggagtctg cgctggctga ctattttgct
      421 gccatcgcca actgctttac cagcaaagac accccggcag gctgcttcat gatcaacaac
      481 tgcaccaccc tctccccaga ttcaggagat atcgccaata cgttgaaatc acgccatgcg
      541 atgcaagagc gcactttgca gcagttttta tgtcaacgac aagcgcgcgg ggaaatcccg
      601 acccactgtg acgtgacaca tctggcagaa ttccttaatt gtattattca ggggatgtcg
      661 atcagcgcac gcgaaggtgc atcgctggaa aaactaatgc agattgccgg aacgactttg
      721 cgtttatggc ccgaactggt gaaataaggc gcaaaaaaag cccctctgcc tgagcgaatg
      781 aggggccaaa ttgcagagta cagccttgtt atactttgtt attcaaaatc agttgcccat
      841 tactatctag cgggatctgc gtacccggat cgcggtccat gcggattttg ccctgctgat
      901 cgccaatctt ataggtcaca tcataaccga gcattttttc tgacttgtca tacaccgttt
      961 tacaacgctg ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc tggatctggt
     1021 taccggcata tccaccaccc agcgccccca caacagtggc gacatcttta ccacgaccac
     1081 caccaaactg atgccctatc acgccgccag caacagcgcc gagcaccgac ccggtaatgc
     1141 gattttcatc ctgcaccggt cgacgatggg tcactgtgac gttgcgacac tcctgacgcg
     1201 gtgttttaac cgtttccttg attggggttg cagaaacaac ctgagcgtat tgcgggcccc
     1261 gttcaaacac gttcagactg gccactgccg ctacgcccag cgcagctgcg acaccaatcc
     1321 cgatacccgc caacattgat ttattcacgg gaacacctcc ttctttgcag ttatgccaat
     1381 catgcaagcg aaggagaaaa gcagctatca gacagaagat gaaaagcgcg ggaatggctg
     1441 gaaatactta aggattcgga ggagtctgaa ggaatgcctg atgcgacgct taacgcgtct
     1501 tatcaggcct accgctcgta ccatttgtcc agggcggatg aggcgtttat gccacatccg
     1561 ccagtgtacg tcgattaatg caacttcaaa cgcggacgga taacgcggtt aatactcccc
     1621 accagcatca ttaatccggt tttaaagtaa ccatgcagcg caatctgatg cattcggtat
     1681 agcgagatat atacaaagcg cgcaattcgt ccttcaatca tcattgagcc gcgcgtcagg
     1741 ttacccatca ggctaccgac ggtggagaag ttcgacagcg ataccagcga accatgatct
     1801 ttatactgat aatttttcag cggcttacca ttcatctgcg ccagaatgtt gttcattgcg
     1861 caagtcgcca tctggtgtgc agcctgagca cgcggcggaa caaagccccc ttccggacgc
     1921 gggcatgacg cgcagtcgcc aatagcgtaa atgtctggat cgcgggtggt ttgcagcgtc
     1981 ggttccacca ccagctggtt gatacggtta gtttcaagac caccgatatc tttcaggaag
     2041 tctggcgctt tgatcccggc tgcccacacc atcagatcag cctcaatata ttcaccatct
     2101 ttagtgtgca ggccgccttc atcagcactg gtgaccatgg tttgcgtcag cacgcgtacg
     2161 ccaagtttcg ttagctcgtt gtgggccgca gcagagatac gtggcggtaa tgcaggcaaa
     2221 atacgttctc ccgcttctac cagcgttacg ttcagggctt cgttggtcag gcctttgtaa
     2281 ccgtagctgt gcagttgctt gaccgcattg tgcaattcag cggagagttc tacacccgtc
     2341 gcgccgccgc cgacaatcgc aatgttcact ttgccattcg cgcccaggtt ggcggagtac
     2401 ttcaggaaca aattcagcat ctcctggtgg aagcgacgcg cctggtgcgg gttatcgagg
     2461 aaaatgcagt tctctttgac acctggcgta ttgaaatcgt tagaggtgct acccagcgcc
     2521 attaccaggg tgtcataggc gattttacgt tccggaacca gcagttcacc tttctcgtcg
     2581 cgcagttctg caatagtgat tgttttcgct tcacgatcaa tatcaatgac ggaacccagc
     2641 tggaactgga aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc gacgccttca
     2701 tcaagcgagc cagtcgccac ttcgtgcagc agcggtttcc acagatggct gtggttacga
     2761 tcgaccagcg taattttggc ttttttcttg cgtcccagct tatgccccag ctgtgttgcc
     2821 atttccagcc caccagcacc gccgccgaca atcacaattt ttttcaatgg cgtagtcaac
     2881 gtg
//
LOCUS       DAZFRN010000305         2864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFRN010000305 DAZFRN010000000
VERSION     DAZFRN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.308"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..92
                     /locus_tag="ACHKUB_005098"
     CDS             <1..92
                     /locus_tag="ACHKUB_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6657964.1"
                     /translation="VMVNTGGNKRQVPAKRQNRGSRNSKDDGG"
     gene            116..418
                     /locus_tag="ACHKUB_005099"
     CDS             116..418
                     /locus_tag="ACHKUB_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657965.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            574..861
                     /locus_tag="ACHKUB_005100"
     CDS             574..861
                     /locus_tag="ACHKUB_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657966.1"
                     /translation="MSTRNIHVNTASYTILVAGREKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            980..1801
                     /locus_tag="ACHKUB_005101"
     CDS             980..1801
                     /locus_tag="ACHKUB_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6657967.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFLPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(2097..2699)
                     /locus_tag="ACHKUB_005102"
     CDS             complement(2097..2699)
                     /locus_tag="ACHKUB_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO6657968.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLMFI
                     NVTCKAGDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTGIKSSKGIRIPVTKKSLPEINSVQNN"
BASE COUNT          792 a          618 c          697 g          757 t
ORIGIN      
        1 aggtgatggt gaataccgga gggaacaaaa ggcaggtccc cgcaaaacgt caaaaccgcg
       61 gctcccgaaa ctcaaaagat gatgggggct gaaaacatca gatacaggag gaaacatgag
      121 cgcattttct tttaacaccg cttatcagcc aacaggcgat cagcagaaag acgcaatcgc
      181 acaaatcgac atcatgcaga atcgtgctgt tcaggcaaat cttgcatacc agagcagcct
      241 ggatgcggaa acgctgttga aacagttatg gcaaatcaat gatgaactgg gggaactgtt
      301 ggatagcgtg gaaaatcaca cgccagttat cagaaaaaaa gccagtgatt tgagtgcact
      361 gatgatgctt ttcagccaac aggccggtca gcctatgacc atgttgttga tttgttaatg
      421 ataatcaggg gcaggaaatt aagcgaaaga tgctctgccc cgggtttgca gcgaccggaa
      481 cggtcgcgcc acccttgtcg cgctgtggcc tgcggcggct cccgaaactc aaccgatgat
      541 gggggctgaa aaccagagag aggagaaagc aggatgagta cccgaaatat ccacgttaac
      601 accgcgtcgt acacgattct tgttgccggg agggaaaaaa acaccgggga agagtgggac
      661 gtcctggaat tcagcagcct gactgagctg aaaaaatatc gcaaaagcca cccggaaaag
      721 atggccttca gctatagcta cgcgctcagc cggggtgtgg atacgcagtt ccgccatatc
      781 aacattgcgg aagccgatca cttcaaacag ttcctgcgtc agattaagcg tgccggcctc
      841 gatatccggg cgatctgctg accggatgtg atgaatatcc ctcctgattg ttgcaatctt
      901 gttaatggag gtcgggaggg atgatattaa gcggaaaaat tttttattaa atttattctt
      961 tcataaagga gtagctgtta tgcgattagc ttcccgtttt ggtcggtata attccatccg
     1021 tcgtgaacgt cctttaacgg atgatgaatt aatgcagttc gtgccttcgg tattttccgg
     1081 tgataaacat gagtcccgga gtgaacgtta tacgtatatt ccaacaatca atatcatcaa
     1141 taagttacgt gatgaaggtt tcctgccgtt ctttgcctgt cagagtcggg ttcgtgattt
     1201 gggacgccgc gaatacagta aacatatgtt acgtcttcgc agggaagggc atattaacgg
     1261 gcaggaagtt cctgaaatta tcctgcttaa ttcgcatgat ggttcatcca gttatcagat
     1321 gatccccgga atttttcgtt ttgtctgcac aaatggcctg gtgtgcggaa ataattttgg
     1381 cgaaatccgt gttccacata aaggtgatat tgtcgggcag gttatcgagg gagcgtatga
     1441 agtgctcggt gtctttgata aggtcactga taatatggag gcgatgaaag aaattcatct
     1501 taacagtgac gagcaacatt tatttggcag agctgcactg atggtcaggt atgaagatga
     1561 aaataaaacg ccagtgacgc ctgaacaaat aattactccc cgtcgtcggg aagataaaca
     1621 gaacgatctc tggacaacct ggcagcgggt tcaggagaat atgataaaag gtggattatc
     1681 ggggcgaagt gcctccggga aaaataccag gacaagagcc attacaggta ttgatggtga
     1741 tataagaatc aacaaggcgt tatgggtgat tgccgaacag ttcagaaagt ggaagtcatg
     1801 ataatataag taaagccctg aaaatttttc ggggctttac ttatattatc attcttcggc
     1861 atcgctgtgt cgtctgatat acctcaattc catcaaccgg ctttccggtt gtctgtaaat
     1921 attgtgcccg cgaaaaatgg tctggcagga ggctgcgcag ttaccgggta tatataagat
     1981 gggttcccgc ttcgcggggc ttcggccagt cagcattgtc actcatatga tatttttgtg
     2041 tgtggccttc catgccgctt ttgcggcata accggtatct gacaatgtct gtaagattaa
     2101 ttgttctgca cgctgttaat ttccggaagt gatttcttcg tgaccggaat ccggatacct
     2161 ttactgcttt ttatcccggt ataaatccgg taaacctctg atgcgtaggc aagacgtctc
     2221 tggttctggc gttcggtctt cctgaatccg gcattgtatg caccaacggc ttcccaggag
     2281 acgccccatt ttttaaaggc tattgccaga taataagcac cggtataaat gttcatgcag
     2341 ggatctgttg tcagatgttc cggtttaatt ccatagcggg ccagttcgtt aaaatgctgg
     2401 gaatctacct gcatcagtcc gctgccatat cccgttaccg gattaatacc gatggcatta
     2461 acccggtaac gggattcttt ccatgatatt gctctcagca agtcagggtc aattttataa
     2521 tctctgccag ccagatcaaa gcaatcacca gccttgcagg tcacatttat aaacatcagg
     2581 cagatcgtca gaagccattt tctcattttc tcacctctgg tgacgtcatc aggaaataat
     2641 ttaacccact ccacaaaaag gctcaacagg ttggtggttc tcaccaccaa aagcaccaca
     2701 ccccacgcaa aaacaagttt ttgctgattt gcgttttaaa tcatgtaatt aataaatgct
     2761 atttatattt aataattctg aattattaaa tagagagtcg ttggcgatcc tgttacgacc
     2821 ctatagcgac tcttattagg atcagtactg acaattttca tgaa
//
LOCUS       DAZFRN010000306         2861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFRN010000306 DAZFRN010000000
VERSION     DAZFRN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.309"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            3..230
                     /locus_tag="ACHKUB_005103"
     CDS             3..230
                     /locus_tag="ACHKUB_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFO6657969.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            580..1155
                     /gene="dolP"
                     /locus_tag="ACHKUB_005104"
     CDS             580..1155
                     /gene="dolP"
                     /locus_tag="ACHKUB_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO6657970.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
     gene            complement(1585..>2861)
                     /locus_tag="ACHKUB_005105"
     CDS             complement(1585..>2861)
                     /locus_tag="ACHKUB_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6657971.1"
                     /translation="RKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAKA
                     NFAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRGA
                     GIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKIISAYNSQGTLSAEETKQLEEVK
                     KERADMFATYMATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQT
                     VTEKADAALDKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEH
                     KLLISENSKQISTNTLGIRENTQRIRDEGQARIRGDRETLRAATGYTDMRVNGLERNT
                     NKRFAQLKEDIDKNRKRADAGIAGAMAMTGIPSVPGKKISFGMAMAGYRSEGAVAAGF
                     HFNTSENSAVKVNAAWDTENGAGVSAGMALGW"
BASE COUNT          727 a          668 c          652 g          814 t
ORIGIN      
        1 caatgttccc ggagtacaga gaactgattt cagcattgaa aggaaatcat aaacgctttg
       61 attctttatt tgaaaagcat aataaacttg atcacgaaat aaaaagaatt gaagatgagt
      121 ttggttcagc gtattcgacg aaagtggtca ggctgaagct tgaaaaactt gagatcaaaa
      181 gagaaatcca gaaaatcctt gaagatgagt cagaaaaggg cgaacggtaa tcataaacgg
      241 tatattcaaa gccgtgtttt ctctgtttta tgaaaaaggc accgggggct ccacggtgcc
      301 ttttagcctg tgtataacga aaaaggagat cctgttcagc aaaccaggta gttaaaaaaa
      361 cagaatcttt acggcgatat caaagttttc gtgcaaaaaa catgatcaat attaaatttc
      421 agtacatcca tatccggcat aaacatttcc gtctggcaga tacagatatg ccacgtaacg
      481 tatccgctgt ttccggctgg ggcggaggag aataacagtc cgtagcttgt tttgtttcac
      541 tgaaggtaaa ctttttgtac gataaattcc gggggagata tgcgtttatt atattctgct
      601 gttgtcctta cttcagcctt attgttacag gggtgcgtgg ctgccgctgt ggtgggtaca
      661 gctgctgtcg ggacaaaggc tgcaaccgac ccacgcagtg tcggaacgca ggtggatgat
      721 gaagtgctgg agctgagggt cagcagtgca ctttcaaaag atgaacaaat cagaaaagaa
      781 gcacgcatca gtgagattgc atatcaggga cgcgtgctgc tgatcggtca gtctccggac
      841 cgtgaattat ctgcccgtgc ccgtcagata gcactcggtg tcgatggtgt tcaggaggtc
      901 tataatgaaa tccggcaggg gactcccgtc agtccggtca taatggcagg tgatgcctgg
      961 ctcacgacaa aaattcgttc tcagttactc acaagtgccc gtgtcaaatc atccagtgtc
     1021 aaggtggtga cagaaaacag cgaagtcttt ttactgggcc tggtcacaga gcaggaaggg
     1081 cgagcggcag cagatattgc cagtagggtg agtggcgtga agcgggtcac gacggcattc
     1141 tcctgtatac ggtgaaggcc gtatctgagt tgcctttagt gcaaatttac gttaaaaatc
     1201 agaagagcac gtttccgggc cgttcatgcc accacgtgaa cattcgtcgc ggaataccgg
     1261 agacgtgctc attataataa ccaatataca taattcctct ttgacacccc cctgaattcg
     1321 ggggattttt ttgcccgggt atgttcactc acagataaaa tcccggtgcg ctccggatgt
     1381 cgctgctggt aaataatctg agcttttatc catacaggtc aaagagcgtg gcgttgacac
     1441 gtgtacgtct ggctccggtg caggagccag attgtccgta ttaattacat cctgagccga
     1501 tgcgataaac tgaatcgctt cctgaatggt ggtgcagtgt cccctggata acaatatctg
     1561 cgaacggctt ctggcccgga agcattacca tcccagagcc atcccggctg aaacgccagc
     1621 tccattttca gtgtcccagg ctgcattcac tttaacagca ctgttttcac ttgtattaaa
     1681 atggaagccg gcagcaacag caccttcact ccggtatccg gccatggcca taccaaatga
     1741 gatctttttc ccgggtacgg acggtatccc cgtcattgcc atcgcacctg caatacctgc
     1801 atctgcacgt tttctgtttt tgtctatatc ctctttaagc tgcgcaaaac gtttattcgt
     1861 attacgctcc agcccgttta cccgcatgtc ggtataaccg gtcgctgcac gcagtgtttc
     1921 cctgtctccc cggattcgag cctgtccttc atcccggatc cgttgtgtat tctctctgat
     1981 accaagagtg ttggtactga tttgtttgct gttctccgaa ataagcagct tatgttcttc
     2041 tgctgtctgt gtattggctg tcactctttc atccagaatt tttgtcgttt tattaatgtt
     2101 actgatttcc tctttattac tgttaaccac ctgcagattt ttagttatcg cactggtgtt
     2161 gtttgttatg cgtttatcaa gcgctgcatc tgccttttct gtcacagtct gcaaatccct
     2221 gagctgtgca acgttaacag catccgtatc gacagaacct gctgcaacgc tagttatctg
     2281 tcttgtaaca gcatgtcgtc ctgttgtcgt tgcaccaaca cctacagata cagcatcgtg
     2341 cgttgccata tatgtcgcaa acatatcagc ccgctctttt ttcacttcct ccagttgttt
     2401 agtctcttca gcagacagcg tcccttgtga attataagcg gatattatct tatcgatttc
     2461 cataagcctg ttaagctgct tgtcagacat cctggatttt actacatccc ctggattcca
     2521 gcgctttgtc tctggcatat atgcttcaat accagcgcca cgatcagcaa cacttcctgc
     2581 gcccagagcg acagagaatg gtttcaatgt tgttgcgttt gctcctaacg cggtagagtt
     2641 attttcaata gctcttgctg atgcacctaa agaaatactt ttttctgcat ttgcctgtga
     2701 atatcgacca acagcaacag cgaaattagc ttttgccgca ctgttatcac caagttccac
     2761 gatcatttta tcaggaatac catttgcaga taagacaggt aaagaagctg agcagtagag
     2821 aaataatgat ttgcatatat aactgacact gattttacgc a
//
LOCUS       DAZFRN010000307         2850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFRN010000307 DAZFRN010000000
VERSION     DAZFRN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.310"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            272..1438
                     /gene="nhaA"
                     /locus_tag="ACHKUB_005106"
     CDS             272..1438
                     /gene="nhaA"
                     /locus_tag="ACHKUB_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO6657972.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1504..2403
                     /gene="nhaR"
                     /locus_tag="ACHKUB_005107"
     CDS             1504..2403
                     /gene="nhaR"
                     /locus_tag="ACHKUB_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO6657973.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2442..>2850)
                     /locus_tag="ACHKUB_005108"
     CDS             complement(2442..>2850)
                     /locus_tag="ACHKUB_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO6657974.1"
                     /translation="DQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVE
                     YYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSNYYSFQHPHEG
                     SNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          676 a          633 c          712 g          829 t
ORIGIN      
        1 ggcctaacaa acatcgccga ctgacaacaa attaattatt acttttccta attaatctct
       61 cagaaatcct caccttaagc tatgattatc tgggcttagg gtcactcgtg agcgcttaca
      121 gccgtcaaaa actcatctca ccgctgatgg cgcaaattct tcaatggatc gtaaaaaacg
      181 agttattcct acactataat ctgattttaa cgatgatttg tgcggggtaa aatagtaaaa
      241 acgatctatt cacctgaaag agaaataaaa agtgaaacat ctgcatcgat tctttagcag
      301 tgatgcctcg ggaggcatta ttcttatcat tgccgctatc ctggcgatga ttatggccaa
      361 tagcggcgca accagtggat ggtatcacga ctttctggag acgccggttc agctccgggt
      421 tggttcactc gaaatcaaca aaaacatgct gttatggata aatgacgcgc tgatggcggt
      481 atttttcctg ttagtcggtc tggaagttaa acgtgaactg atgcaaggat cgctagccag
      541 cttacgccag gccgcatttc cagttatcgc cgctattggt gggatgattg ttccggcatt
      601 actctatctg gcctttaact atgccgatcc gattacccgc gaagggtggg cgatcccggc
      661 ggctactgac attgcctttg cacttggtgt gctggcgctg ttgggaagtc gtgttccgtt
      721 agcgctgaag atctttttga tggctctggc tattatcgac gatcttgggg ccatcattat
      781 catcgcattg ttctacacta atgacttatc gatggcctct cttggcgtcg cggctgtagc
      841 aattgcggta ctcgcggtat tgaatctgtg tggtgtacgc cgcacgggcg tctatattct
      901 ggttggcgta gtgttgtgga ctgcggtgtt gaaatcgggg gttcacgcaa ccctggcggg
      961 ggtaattgtc ggcttcttta ttcctttgaa agagaagcat gggcgttctc cagcgaagcg
     1021 actggagcat gtgttacacc cgtgggtggc gtatctgatt ttgccgctgt ttgcatttgc
     1081 taatgctggc gtttcactgc aaggtgtcac gctggatggc ttgacctcca ttctgccatt
     1141 ggggatcatc gctggcttgc tgattggcaa accgctgggg attagtctgt tctgctggtt
     1201 ggcgctgcgt ttgaaactgg cgcatctgcc tgagggaacg acttatcagc aaattatggc
     1261 ggtggggatc ctgtgcggta tcggttttac tatgtctatc tttattgcca gcctggcctt
     1321 tggtagcgta gatccagaac tgattaactg ggcgaaactc ggtatcctgg tcggttctat
     1381 ctcttcggcg gtaattggat atagctggtt acgcgttcgt ttgcgtccat cagtttgaca
     1441 ggacggttta ccggggagcc ataaacggct cccttttcat tgttatcagg gagagaaatg
     1501 agcatgtctc atatcaatta caaccacttg tattacttct ggcatgtcta taaagaaggt
     1561 tccgtggttg gcgcagcgga ggcgctttat ttaactccac aaaccattac cggacagatt
     1621 cgagcgctgg aagagcgcct gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa
     1681 cccagcgagc tgggagaact ggtctatcgc tatgccgata aaatgttcac cttaagccag
     1741 gaaatgctgg atattgtgaa ctatcgcaaa gaatccaatt tattgtttga cgttggtgtg
     1801 gctgatgcac tttccaaacg cctggtcagt agcgtactta acgccgcagt ggtagaaggc
     1861 gagcccattc atcttcgctg cttcgaatcc acccacgaaa tgctgctgga gcaattaagt
     1921 cagcataaac tggatatgat catttctgac tgtccgatag actctacgca gcaggaaggc
     1981 ctgttctccg tgagaattgg cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca
     2041 gaaaaaccgt tcccggcttg tctggaagaa cggcgacttt tgattcctgg gcgacgttca
     2101 atgttagggc gcaaattgct taactggttt aactcccagg gattaaacgt agaaatcctc
     2161 ggcgagtttg atgatgccgc gttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc
     2221 gttgccccaa cgctttatgc atatgacttt tatgccgata aaactgtcgt agaaattggt
     2281 cgcgtcgaga atgtgatgga agagtaccat gctatttttg ctgagcggat gattcagcac
     2341 ccggcggtac agcgaatctg caatacggat tattctgcgc tttttagtcc agcggcgcgt
     2401 taatcggcac ctccccccaa agttaaggtg ggggagatag attagttgta cataaccacg
     2461 attttgactc ggctcattat ttgcccgctt gagacattat ttccatatgt acgcgggcga
     2521 ataaatagag gaatctgatt acttccttca tggggatgct gaaaagagta gtaattgctt
     2581 ttaccatact ggatttcatt cgtaccatca ttggagagtt gaaagcctac ttgacccgca
     2641 ccgtcctcac tgttttctaa ctcatttttt aatatgctgg ttcctgactc ctgtatcgtc
     2701 actggttcaa agtaatattc aacatcactg ggttcaaaaa ctaattcacc ttgctcgccc
     2761 tggatgcagt caaatcttat atgatgaaac tgataatttt cattattgat acgagacagt
     2821 acaaatcgtc cagtgtcgat ggattgatct
//
LOCUS       DAZFRN010000308         2843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFRN010000308 DAZFRN010000000
VERSION     DAZFRN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.311"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            244..969
                     /locus_tag="ACHKUB_005109"
     CDS             244..969
                     /locus_tag="ACHKUB_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6657975.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            927..1604
                     /gene="bglJ"
                     /locus_tag="ACHKUB_005110"
     CDS             927..1604
                     /gene="bglJ"
                     /locus_tag="ACHKUB_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO6657976.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERRHGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1642..2430)
                     /gene="fhuF"
                     /locus_tag="ACHKUB_005111"
     CDS             complement(1642..2430)
                     /gene="fhuF"
                     /locus_tag="ACHKUB_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO6657977.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLSTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2571..2807
                     /locus_tag="ACHKUB_005112"
     CDS             2571..2807
                     /locus_tag="ACHKUB_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO6657978.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          760 a          605 c          706 g          772 t
ORIGIN      
        1 taagtattcc taacaactta atgtagatat tttaaatgtc tccaggctat ttcaccaggt
       61 tatcatttga tgctaacctt ctgacacatg cagtggagtt gttgtgcagc aggagtatgc
      121 tgatatgaaa gtgaatgcta aggataattt attcgcttaa tctattaatt tgctggaaac
      181 atttaaggaa tgaatatcct gaataaacca tcatgatttt tagacagtgg atgtggagga
      241 aatatgttgc caggatgctg caaaaatgga attgttatca gtaaaatacc tgttatgcaa
      301 gcagggttaa aagaggtcat gaggactcac ttccctgaat atgaaataat atccagcgcc
      361 tctgcggagg accttacctt attacaatta cgtcgttccg gattagtcat tgctgattta
      421 gccggtgaaa gtgaagatcc acgttctgtt tgtgaacatt attattcttt aatctcacaa
      481 tatcgggaaa ttcactgggt tttcatggtg tcacgctcct ggtattccca ggcagtagaa
      541 ttgctcatgt gccctacggc gacgttattg tctgatgttg aacccattga gaatctggtc
      601 aagaccgtac gttccggcaa tacgcacgca gagcgtatca gcgccatgct gacctctccg
      661 gcaatgactg aaactcatga ttttagctat cgctccgtca ttctcactct ttcagagcgc
      721 aaggtactgc ggctattagg taaaggatgg ggcatcaacc agatagcttc gttgcttaag
      781 aaaagtaata aaactatcag cgcccaaaaa aacagtgcga tgcgtcgact ggcaattcac
      841 agcaacgctg aaatgtatgc atggataaat agcgcgcagg gtgcaagaga acttaacttg
      901 ccttctgttt atggagatgc cgcagaatgg aacacagccg aattaagaag agaaatgtcg
      961 cactcataga aaaatgcgtc atgagcagta tcggtattga gagtttattc agaaagtttg
     1021 cgggtaaccc ttataagctc catacctata ccagtcagga gtcatttcag gatgccatgt
     1081 cgcggatctc gtttgcggcg gtcatttttt ctttttctgc catgagaagt gagcgcagac
     1141 atggattatc ttgcctgact gaactggcga ttaagtttcc gcgtacccgg cgtttagtta
     1201 ttgcggatga tgatattgaa gctcggctga ttggttcatt gtcgccatca ccgctggacg
     1261 gtgtattaag taaagcgtca acgctggaga tttttcatca ggaactcttt ttgtcattaa
     1321 atggtgtacg tcaggcgacc gaccgactga acaatcagtg gtacattaac caaagccgga
     1381 cgctaagccc gacggagaga gaaatattgc gctttatgtc gcgtggctac tcaatgacac
     1441 aaattgccga gcagcttaaa cgcaatatca aaacgatccg tgcacataaa tttaatgtga
     1501 tgtcgaaact gggcgtcagt tctgatgcag ggttgttgga ggccgcagat attctgttat
     1561 gtatgcggca ttgcgaagca agtaatgtgt tgcatcccta ttaatccgca ttatgccggg
     1621 tttacttccc cggcagtgct ttcatttcag cgtacaatcg ccacattgct gcacatccgg
     1681 taagcgataa cgctggcagc aagtgcggcg caccagcagg ccgtcgcgca gtaccacggt
     1741 acgccagagt ggattatctt caccggtaga aagcgttttc tcaaaaaaga gggcatggcg
     1801 cagcgattca accgtcgcct cgccgagcag ttgcttcatc tcagtgagat accagttgat
     1861 caaataaccg gtattactcc agataagttt gccgttgatc tctccggtcg cttctagtgc
     1921 ctgcacaacc ggaaccagcg cctggctgat taacgtttcc attcgctgct gcggcgaatg
     1981 tggtgttgcg tttttatctt cacacacatc gacccagaaa caggcggcac ggccggtttc
     2041 gtgaaactca gcatggaaat gttccggcga cacatctaat gccttttcct gcgtcagtag
     2101 cgccagcatt aatggtggca ccatcaggcc gatataccat tgtgcccata gcgagatcag
     2161 cggtttgttc tcgcggatca tcgttggttg gttgcgatag atatgatcgg aatagaccgc
     2221 gagtagagaa cttagcgcat tcggtgatga ccattgcgcc agcgtcatgg cgttaagcgg
     2281 agcgggttca tccaggcgga taaactccag caaatgttca cgatgtttgg cgatcgtcgc
     2341 acgtacggct tgtgcaagcc cagcatcctg tggctggaga tgcgttcgcc agataacatc
     2401 ttcatagagc ggtgcggaac gataggccat aatcgggata gtaatctaaa tgataatgat
     2461 tgctaatcct agcgataggt ttatccgata gcaagggatt tctctggctt gcaaatgata
     2521 aaaattatca tatgatattg gttatcatta tcaatgaaag agatgaaacc atgttgcaac
     2581 gtacgctggg cagtggctgg ggagtgttgc tgccaggatt gctgattgca gggctgatgt
     2641 atgcggattt atcgcctgat caatggcgga ttgtcattct gatgggatta gtattgacgc
     2701 cggtgatgct gtatcacaaa cagttacggc attacgtttt gctaccatcg tgcctggcgc
     2761 ttattgctgg catcatgctg atgataatga atttgaatca gggatgaaaa atcaaggaag
     2821 aaacaagaaa ggaagtaaag ata
//
LOCUS       DAZFRN010000309         2783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFRN010000309 DAZFRN010000000
VERSION     DAZFRN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.312"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            82..2361
                     /gene="ydeP"
                     /locus_tag="ACHKUB_005113"
     CDS             82..2361
                     /gene="ydeP"
                     /locus_tag="ACHKUB_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO6657979.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2609..>2783
                     /gene="safA"
                     /locus_tag="ACHKUB_005114"
     CDS             2609..>2783
                     /gene="safA"
                     /locus_tag="ACHKUB_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO6657980.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHF"
BASE COUNT          734 a          652 c          682 g          715 t
ORIGIN      
        1 gttggcgata atccctttgt atgtactgtg catcatcgct attacaaatc ctaataattc
       61 atttccacac aggataagta gatgaagaaa aaaattgaat cctaccaggg tgctgcaggt
      121 ggttggggtg ctgttaaatc cgtagcgaat gcagtacgta agcagatgga tatacgccag
      181 gatgttattg ccatgtttga catgaataag ccagagggct ttgactgtcc gggttgtgca
      241 tggccagatc ctaagcacag tgcgtcattc gacatttgtg aaaacggcgc aaaagcaatc
      301 gcctgggaag tcacggataa gcaggtaaat gcctctttct ttgctgagaa tacggttcaa
      361 tcattactta cctggggaga tcacgagctt gaggctgcgg gacgtctcac tcagcctttg
      421 aaatatgatg ccgtcagcga ctgttacaag ccattaagct ggcaacaagc tttcgacgaa
      481 attggcgcac gccttcaaag ctatagtgat cccaatcagg ttgaattcta tacttcgggc
      541 cgcacttcca atgaagctgc ctttctttat cagctttttg cccgtgaata cgggagcaat
      601 aactttcccg actgctccaa catgtgccat gaaccgacca gtgtgggttt ggcagcgagt
      661 atcggcgtgg gtaaagggac tgtgttgctg gaagactttg agaagtgcga tttagtcatt
      721 tgcattgggc ataaccctgg tacaaaccac cctcgcatgc tgacttcgtt gcgcgcttta
      781 gtgaaacggg gagcgaaaat gatcgccatc aatcctctac aggaacgtgg cctggagcga
      841 tttaccgcac cgcaaaaccc gtttgaaatg ctgacgaact ctgagactca gttggccagt
      901 gcctactaca acgtgcgcat tggtggcgat atggcgttgc tcaaggggat gatgcgcctg
      961 ttaattgagc gcgatgatgc tgcaagtgcc gcaggtcggc cctcattgct tgatgacgaa
     1021 tttattcaaa cgcataccgt cggctttgac gagctacgcc gtgacgttct caattccgag
     1081 tggaaagata tcgaacgtat ttctggacta agtcagacac aaatcgccga actggctgac
     1141 gcatatgccg ctgccgaacg caccattatc tgttacggaa tggggatcac tcagcacgaa
     1201 catggtaccc agaacgtaca gcaactggtc aatttgctgt tgatgaaagg taacattggc
     1261 aagcctggtg cgggtatctg cccactacgt ggtcactcta atgtacaggg cgaccgaacc
     1321 gtcggtatca ccgagaaacc gtctgcagag tttctggctc gcctgggtga gcgctatggc
     1381 ttcaccccac ctcatgcacc tggacatgct gcaattgcca gcatgcaagc aatatgtacg
     1441 gggcaggctc gagcattgat ctgcatgggg ggcaactttg cactggcaat gccagatcgg
     1501 gaagcgagcg ctgtaccgtt aacgcaatta gatttggcgg tacacgtagc cactaagctt
     1561 aaccgctctc atctgctgac cgcacggcat agctatattc tgccggttct ggggcgtagc
     1621 gagattgata tgcaaaaaag cggtgcgcag gcggtaaccg ttgaggattc aatgtcgatg
     1681 attcatgcct cgcgtggcgt gttaaaaccc gccggtgtaa tgctgaaatc agagtgtgct
     1741 gtggtcgcgg gaatcgccca ggcaacacta ccccagagcg tggtagcctg ggagtatctg
     1801 gtggaagatt atgatcgcat tcgcaatgac attgaagctg tgctgccaga gttcgccgac
     1861 tataaccagc gcatccgtca tcccggtggt tttcacctga taaatgcagc tgctgaaagg
     1921 cgctggatga cgccgtcagg taaggctaat ttcattacca gcaaagggct gttagaagat
     1981 ccctcttcag cgtttaacag taagctggtc atggcgacag tacgcagcca cgatcagtac
     2041 aacacgacga tttatggtat ggatgatcgc tatcgagggg tattcggtca acgagatgtg
     2101 gtctttatga gtgctaaaca agctaaaatt tgccgtgtaa aaaacggcga aagagttaat
     2161 cttattgcgc ttacgccaga cggtaagcgc agctcacgcc gcatggatag attaaaagtg
     2221 gtcatttacc ctatggctga ccgctcactg gtgacctatt ttccagaatc gaatcacatg
     2281 ctaactcttg ataaccacga tccattaagt ggcattcctg gttataaaag tattccggtt
     2341 gaattagaac catcaaatta attcgcctcc tcatttcttc tgctgtcatc cgcacagcag
     2401 aagaatttct cattgactat tatcgcgcaa tttgctcaca ttgattaaat taaaccacac
     2461 actataagat ataaacctct gcctacagct gtaagaaact ccgctcagta ctgaagtgcc
     2521 agtcctattt cctcttttct ccagcctgtt atattaagca tactgattaa cgatttttaa
     2581 cgttatccgc taaataaaca tatttgaaat gcatgcgacc acagtgaaaa acaaaatcac
     2641 gcaaagagac aactataaag aaatcatgtc tgtaattgtg gttgtcttat tactgaccct
     2701 tacgttgata gccatttttt cggcaattga tcagctgggt atttcagaaa tgggtcgcat
     2761 tgcaagagat cttacacatt tca
//
LOCUS       DAZFRN010000310         2783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFRN010000310 DAZFRN010000000
VERSION     DAZFRN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.313"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            73..372
                     /locus_tag="ACHKUB_005115"
     CDS             73..372
                     /locus_tag="ACHKUB_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HFO6657981.1"
                     /translation="MNKMFSQWIDFDEMITPKIIKVIYWFFLFITVAGGVIGMIANPF
                     VGLLGLIMGIIGLRIGCEMIILLFNIYKQVKKIADAVPDKINEQAEDSLLSQNKH"
     gene            1475..2377
                     /locus_tag="ACHKUB_005116"
     CDS             1475..2377
                     /locus_tag="ACHKUB_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO6657982.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            2410..2733
                     /locus_tag="ACHKUB_005117"
     CDS             2410..2733
                     /locus_tag="ACHKUB_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6657983.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
BASE COUNT          771 a          491 c          690 g          831 t
ORIGIN      
        1 tttgtatttt acgcgttagc agtgttttta ttttgacatt gctgttaaca accgattgct
       61 ggagaggtga aaatgaataa aatgttcagt cagtggattg attttgatga gatgattact
      121 ccgaagatca tcaaggtaat atactggttt tttcttttta ttacagtggc cggtggtgtt
      181 ataggtatga tagccaatcc gtttgtcgga ctgcttggtc ttattatggg tattattggt
      241 ctgaggattg ggtgtgaaat gataattctt ctttttaata tttataagca ggttaaaaag
      301 attgctgatg cggtaccgga taaaataaac gaacaggctg aggatagttt attatctcag
      361 aataaacatt gatgaatggt aataacaggg actgttaata ctgtccctgt tattttttct
      421 gcagactgtt ttctgttctt cctggttatg ttctgagtga tgatgattga cgccttttat
      481 acgctctgat atgctcggaa agcattatct gtcgctttgg cgaccaccaa tgctccataa
      541 gtggtctgag aggagcgcct tcgggtgacc acatcccgct tcggtgggca ccaatgttcc
      601 gttgatctga gaggaacgcc ttcgggtgat caacaccccc gtaacctgaa aggggatgtg
      661 cagccaggat gtttctggtg gtggtaaatc tgcccacctt tgagagcaga tatcgtcagt
      721 catgatgatg aaggtttctt ttaaccgcgc cctttattac gggcaataca cagcagtggt
      781 tgtgtgatcc ttatctatcc ggtgatgccg gaatgttttt actgcgtaat ggcggtattg
      841 acttctttta ttcttttcat ttgtttgcca gcacttcctg cgactgtgcg cgctgtataa
      901 gaccatttga aatttggtcg cttacgcgtg tacctggcgt ctgaagctgc gcggcgtcac
      961 tttcgtcagc gtgtcacgat gacaaagtga cgcacgcgcg tctgcttaac gaacccgcac
     1021 gaactgccgt ttcacgtgat gttttttaac atttgaagaa gtgacccggc gtgagagtgt
     1081 tttttcaacc tcttctgcaa atttacggac gattttgtga ataacgtcca taaatggttt
     1141 tttgcagaat caattgtttt ttaaggagtt ttatgtagtg agatgtcgaa tcggcgatac
     1201 cacttattag aggtggtgtc gggggcgacc tggagacagg tacagcaaat gctgcagtcg
     1261 gtgtgcaaca ccgatttgtt acccctcaat acctaaacaa tctgcgctac cctggcgcac
     1321 aggcagtcac tacccgaagg atcgcaacgt ttaaccgtgt cgtcagggag aaatgcagat
     1381 gagtttttct gttgtgttca cggcacaaca gaaaaactca tctgccgaac gtacagctgt
     1441 ttaaggtaaa aattattatg tgcaggagaa gttgatgtcc agacgtgaaa aattactgaa
     1501 acaggatctg gttgctcaca ttcgaaaaga caaaggcagc ttcaagaagc tgagtgatta
     1561 caccaggatc atgcaccgct ttgctgaaag tctggcaaaa atgaatgtac agatcagttc
     1621 tgccgcgcag gtaaaagtcc ggcatattga gctatatatg agaagccgga gtaatgtttc
     1681 tgggcgaaca agggaaaatg aaatgtctgc aatacgggtt atgttacgtc aggcaggtaa
     1741 atataagatg gctgatcccg aacatcctcg tctcagcaat aaagcgttag gcatttccgg
     1801 ctccagtcgt aagggaacaa aaaaattaat cacggatgag cgttttcggg agattcttca
     1861 gcgtgtggaa gcaaaagata aaggggtggc agctgttgtt aaactgtcac gttacctcgg
     1921 tttgagaaat gaagaagcta tccagtcggc caaatcatta cgaacctgga aaaaagcgtt
     1981 acaacgggga gatgaaaaaa taagagtgat ttttggtacg aaagggggac gggaaagaat
     2041 gacaacggtt gttgaccggg ataacgttct tgatgctgtt aattatgcac tcaaatatgc
     2101 cagtgaacat aatggacgtc ttatagacag agataatctt aagtcggcaa tggattatta
     2161 tcttaataca ttacgtcgtt atggagaact gaaaggcgga gatgagactg cacacagttt
     2221 tcggtacagt tacgctgcaa gtgccatgaa atatcatatt atgaacggtt acagttataa
     2281 ggaggcactt gcactgacat caattgatct tggtcatggt gatggcagag gacgatatat
     2341 tgaacaggtt tattgtcagt tgttaggcta tgagtaataa ctcatggcga tttcgtacgg
     2401 gtaaatatta tggattgtac tattttgctg aaaataatct ctgtaaaaga gattacaaaa
     2461 aattcgcctt tgtggtcaca ctatctggcg cgatatgttg attccggata tcaggccgag
     2521 tttgaatgtg aaggaacagc agataaatgg gatatctatg atgaatggga catctatgaa
     2581 tccattgcgg attattttga tgtggtggga gattttaact gtgatgtcag atggtcggga
     2641 agaaaaatac tgattaaagg gacctgtgtt tttcctctgg gagaacccgt taagggaata
     2701 cataatattc ctctttggat tgatgttgaa taacttgaca ttcatctttt gtgatttaaa
     2761 aatatacagg cttatatgtc gct
//
LOCUS       DAZFRN010000311         2781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFRN010000311 DAZFRN010000000
VERSION     DAZFRN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.314"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(366..1352)
                     /locus_tag="ACHKUB_005118"
     CDS             complement(366..1352)
                     /locus_tag="ACHKUB_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6657984.1"
                     /translation="MYRYDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            1453..2187
                     /gene="nanR"
                     /locus_tag="ACHKUB_005119"
     CDS             1453..2187
                     /gene="nanR"
                     /locus_tag="ACHKUB_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO6657985.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(2223..>2781)
                     /locus_tag="ACHKUB_005120"
     CDS             complement(2223..>2781)
                     /locus_tag="ACHKUB_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6657986.1"
                     /translation="NKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFSAGGHLASTFV
                     NQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPDSRKQLLGDNSTSENIIQ
                     YSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSV
                     QWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          874 a          627 c          503 g          777 t
ORIGIN      
        1 acctgcgcca catcgactga atgttttggc cctgacagaa aaaagaacta ataggtgatg
       61 tttccgtcca gagatactct ggatggagtg tcggcgtcag tgatataaga ctcgagtgaa
      121 cactgagccg acctcgaaat gacgttgtag gtgcagacat gccataatgg gactgtcgat
      181 gatattacag ggactcacgt ccctggtgcg tgccttcgtg acacactacc gtccacatag
      241 tatcagaacc agtgatatca aacggtaacg accagctatc ttggtgttga taccgacaac
      301 tccataccac tccgtcatgt ctctcagaga atcatgtcag agctgtagat atatcctgaa
      361 cttgtctaca aataatttct tattttttgc gcataacatt cagcaataac tccccttctg
      421 gccattgaat taaagtgtgt acttctctgt gacgttgtcc agcttaattc tgtcctccat
      481 gatgacccca gatacccgac agataaatca tcggcatctt cttctggctc attagttaat
      541 tcatgtcggt tactatcctg aaagctgaga aaatgtattt catcatcagt ccgttgtctg
      601 taatgtccat atataaaatc atattctttt tgataagatt ccttccagta ccaggttgta
      661 tcaccacaca accatggaac tctttctgac gaattaccaa cacactgaga tgagtattca
      721 gataactcag acctgaaaga agttaccatc tgataaaaca agtcaggatg cttttcataa
      781 tctggtgaca taagatcaaa ctccccctgc atccagcaga cagatagcag tttattcttt
      841 ctgttatatt ccagagctac cttaacccgg gctactaagt cctcatagag tgctgttccg
      901 gtgccccacc gcattgcatc agctgttgcc cctgtatcag gtacatacat accttcagct
      961 ccaacagtaa aggcggcacc tcctctgcaa caaggaacca gtaatatccc tgcttgctca
     1021 ggtatgtatg gcagtaactt tttagctata tgcaatccct gtccgacaca accatattcc
     1081 cctttatgca aatcagcagc cggatggtga tatccactca tatcctgaac atcatgcagg
     1141 cagtgatctg ccggaataat ctcattgaat ttacactctt ttcctcctgg tgtgattgtt
     1201 tttcgacgag ccagttgttt tacacgggac tcgggtttgt cgagtgtatc tggcagggga
     1261 ataccttcac cgtaggccat accattagac tgtcctgcga gggctatcac ataccagaaa
     1321 tctatttttt cattaaaatc ataacgatac atcactcccc cctttacata atagatttaa
     1381 ttaaatattt tcattattaa aagaaagtgt taaattaaaa actcatttgc tctaatccat
     1441 ataaatcaat atatgtccga caacaaaaaa accgtgttac ctgggctaac aacgaccaga
     1501 aagcccattg ttacaaaaaa actttcagaa atcgttgccg aagagcttga aagaatgatc
     1561 cgccaagagg agataaggga aggtgaatac ctgccttctg aacgtgaact tatggcattc
     1621 tttggcgttg gaagaccatc tgtcagagag gctttatcat ctctcagaca taaagggcta
     1681 atccggtata acaagggaga aaaagccagt gtctgccgtc catcaccaga aaccatcatt
     1741 aatgggttat caggcattgt taaagatttt ttatccacag ctgatggcat caaggatttt
     1801 gaaagactac gcatcttttt tgaaaccaac cttgtaagat atgcagcaaa aaatgcaacg
     1861 cctgaggata ttcacaggct tgagaaggtg ctcagtatcc atcataactc actgagaacc
     1921 cccgaagtgt atgctcaata tgatattgaa tttcatagta tacttgctga tatacctgac
     1981 aataaagtat taaaaacaat acactcagca ttttctgaat ggctgataat ggccaaacct
     2041 ctggaaaaga ataaaatgac atatgaaaat aacaaaaaaa cccatcagca gcatattgaa
     2101 atacttacgg ccataaaaga gcatgattgt gataaagctg aacagctaat gtataatcac
     2161 ctttataaac gaattgaagc atcatagtta ctcgacgctt caccaggtag tcaccataaa
     2221 tattatttaa atccatgaga atacatccag tccataaaca gttttatcca tcctccaaca
     2281 ggaagatctg tggcccactg aacactaaag ccatgccctc ctttctcata gagatgcatt
     2341 tcagcagaaa cgcctttctc tattaggcca cggtacatga gcagactgtt ttcaattgac
     2401 acaagatcat catcacaaca atggagtaaa aacacaggag gaaaacatga tgtgacatta
     2461 agatccgcac tatattgtat tatattttct gaggtactat tgtcccccag taattgcttc
     2521 ctggagtctg gatgtgtcac atcatctttc attgatatta caggataaat taacccgaca
     2581 aaatcgggaa tagcgaaaat ctcttgttct ttactgtcag aagaacgttt gaaatcacaa
     2641 atctgattca caaacgttga ggcgagatgc cccccagcag aaaatcctaa tacaccaata
     2701 cgatgacgct gtagatgcca tttatcagca ttcttacgaa tataaagcat tgcagatatt
     2761 gcatcaataa aagttttgtt t
//
LOCUS       DAZFRN010000312         2780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFRN010000312 DAZFRN010000000
VERSION     DAZFRN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.315"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>2780
                     /locus_tag="ACHKUB_005121"
     CDS             <1..>2780
                     /locus_tag="ACHKUB_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Contact-dependent inhibitor A"
                     /protein_id="HFO6657987.1"
                     /translation="GRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATE
                     VNAALHAKDLTVTAGANRVTADGRVRALKGEGDVPKVAVDTGALGGMYARRIHLTSTE
                     SGVGVNLGNLYAREGDIILSSSGKLVLKNSLAGGNTTVTGTDVSLSGDNKAGGNLSVT
                     GTTGLTLNQSRLVTDKNLVLSSSGQIVQNGGELTAGQNAMLSAQHLNQTSGAVNAAEN
                     VTLTTTGDTTLKGRSVAGKILTVSSGSLNNGGTLVAGRDATVKTGTFSNTGAVQGNGL
                     KVTATDLTSTGSIKSGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLVSDDVL
                     TLSATQINNSGTLSGAKELVASADTLTTTEKSVTNSDGNLMLNSASSTLAGETSAGGT
                     VSVKGNSLKTTTTAQTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPS
                     LSLSAPELTSSGVLVASALNTQSQTLTNSGLLQGEASLTVNTQRFDNQQNGTLYSAAD
                     LTLDIPDIRNSGLITGDNGLTLNTASLSNPGKITADTLNVRATTLDGDGLLQGAAALA
                     LAGDTLSQGSHGRWLTAGDLSLRGKTLNTAGTTQGQNLTVQADNWANSGSVLATGNLT
                     ASATGQLTSTGDIMSQGDTTLNAATMDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTI
                     RQNGVTNSGTLTGIAALTLAARMDMASPQPALMNNGGSLLTSGDLTITAGSITSSGHW
                     QGKRVLITADSLANSGAIQAADSLTARLTGALVSTAGSKVTSNGEMALSALNLSNSGQ
                     WIAKNLTLKANSLTSAGDITGVDSLTLTVNQTLNNHASGKLLSAGVLTLKADSVTNDG
                     QFQGNATTITAGQLTNGGHLQGETLTLAASGGVNNRSGGVLMSRNALNVSTATLSNQG
                     TIQGGGGVFLNST"
BASE COUNT          688 a          804 c          809 g          479 t
ORIGIN      
        1 ggcagacctg tgatgaatgc cgacggcagc ctgcaggcgc tggaggtgac tgaaggcagt
       61 atcaccatca atggcgcggg cctggacggc acccggagcg atgctgtatc cattattgcc
      121 cgtgcaacgg aagtgaatgc cgcgcttcat gcgaaggatt taactgtcac tgcaggcgct
      181 aaccgtgtaa ctgcagatgg tcgtgtcaga gccctgaagg gcgaaggtga tgtgccgaaa
      241 gttgccgttg acaccggcgc gctcggtgga atgtacgcca ggcgtattca tctgacctcc
      301 actgaaagtg gtgtcggggt taatcttggt aacctttatg cccgcgaggg cgatatcata
      361 ctgagcagtt ccggaaaact ggtcctgaag aacagccttg ccggcggcaa taccaccgta
      421 accggaacgg atgtctcact ttcaggggat aacaaagccg gaggaaatct cagcgttacc
      481 gggacaacgg gactgacact gaatcagtcc cgtctggtga cggataaaaa tctggtgctg
      541 tcttcatccg ggcagattgt acagaacggt ggtgaactga ctgccggaca gaacgccatg
      601 ctcagtgcac agcacctgaa ccagacttcc ggggccgtga atgcagctga aaatgtcacc
      661 cttaccacca cgggtgatac cacactgaaa ggccgcagcg ttgccgggaa aatactcacc
      721 gtcagttccg gcagcctgaa caacggcggg acactggttg ccgggcgcga tgccacggtg
      781 aaaaccggga cattcagtaa taccggtgcc gtccagggga acggcctgaa agtcaccgcc
      841 actgacctga ccagcaccgg cagtattaaa agtggcagca cactcgatat cagtgcccgc
      901 aatgccacac tgtccggtga tgccggagca aaagacagcg cccgcgttac cgtcagcggt
      961 acactcgaaa accgcggcag actcgtcagc gatgacgtgc tgacgctcag tgccacgcag
     1021 ataaacaaca gcggtaccct ctccggggca aaggaacttg tggcttctgc agacacactg
     1081 accaccacag aaaaatcagt cacaaacagt gacggtaacc tcatgctgaa cagcgcgtct
     1141 tccacactgg cgggtgaaac cagtgcgggt ggcacggtgt ctgtaaaagg caacagtctg
     1201 aagaccacga ccactgcgca gacgcagggc aacagtgtca gcgtggatgt gcagaacgca
     1261 cagcttgacg ggacacaggc tgccagagac atcctcaccc tgaacgccag tgaaaagctc
     1321 acccacagcg ggaaaagcag cgccccgtcg ctcagcctca gtgcgccgga actgaccagc
     1381 agcggcgtac ttgttgcttc cgccctgaat acacagtcac agaccctgac caacagcggt
     1441 ctgttgcagg gggaggcctc actcaccgtt aacacacaga ggtttgataa tcagcagaac
     1501 ggcacgctgt acagtgctgc agacctgaca ctggatatac cggacatccg caacagcggg
     1561 cttatcaccg gtgataatgg tttaacgtta aacactgcct ccctcagcaa tccgggtaaa
     1621 atcaccgctg acacgctgaa cgtcagggcg accacgctgg atggcgatgg cctgttgcag
     1681 ggcgccgctg cactggcgct tgcaggcgac accctctcac agggtagtca cggacgctgg
     1741 ctgacggcgg gcgacctctc cctccggggt aaaacactga ataccgcagg gaccacgcag
     1801 ggacagaatc tcaccgtgca ggcggacaac tgggcgaaca gtggttccgt gttggcaacc
     1861 ggtaacctta ctgcttcggc aaccggtcag ttgaccagta ccggcgatat catgagccag
     1921 ggggacacca cgctgaacgc agccaccatg gacaaccggg gcagtctgct ttcggccggc
     1981 acgctctccc ttgatggaaa ctcactggat aacagcggca ctgtccaggg taaccatgtc
     2041 acgattcgcc agaacggtgt caccaacagt ggcacgctca ccgggatagc cgcgctgacg
     2101 cttgccgccc gtatggatat ggcatcccct caacctgcgc tgatgaataa cggaggctca
     2161 ttgctgacca gcggcgatct gacaatcacc gcaggcagta ttaccagttc cggacactgg
     2221 cagggcaaac gggtgctgat caccgcagac agtctggcaa acagcggggc gatccaggcg
     2281 gctgacagtc tgactgcacg tctgacgggt gcgcttgtca gcacagcagg tagcaaagtc
     2341 acctcgaacg gtgaaatggc gctcagtgca ctgaatttaa gcaacagcgg acaatggatt
     2401 gcaaaaaatc tgaccctgaa ggcgaactca ctgaccagtg cgggtgacat caccggtgtg
     2461 gattctctca cgctcacggt gaatcagacg ctgaacaatc acgcgagcgg aaaactgctc
     2521 agtgcaggtg tgctgacgct gaaggcagac agtgtcacaa acgacgggca attccaggga
     2581 aatgccacca ccatcacggc aggacaactc acaaacggcg ggcatctgca gggcgaaacg
     2641 ctgacgctgg ccgcctccgg cggcgtgaac aaccgttccg gtggtgttct gatgagccgg
     2701 aatgcactga atgtcagtac tgcgaccctg agtaaccagg gcacgataca gggtggtggc
     2761 ggggttttcc tgaactccac
//
LOCUS       DAZFRN010000313         2731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFRN010000313 DAZFRN010000000
VERSION     DAZFRN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.316"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..338)
                     /locus_tag="ACHKUB_005122"
     CDS             complement(<1..338)
                     /locus_tag="ACHKUB_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO6657988.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILP"
     gene            1605..>2731
                     /locus_tag="ACHKUB_005123"
     CDS             1605..>2731
                     /locus_tag="ACHKUB_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO6657989.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAV"
BASE COUNT          726 a          529 c          660 g          816 t
ORIGIN      
        1 ggcagaatgt cacggtacag ggcctcatac tctgcatccc tgtcccggct ctcgcccacc
       61 ccgggcaggt cagtgataac catgctgtga ccgtgaccac tcagacggaa gcgctgcact
      121 tcccgggtgc cggcgtgaac atcactgacc ggtgtgacct ccccctgaaa cagtgcatta
      181 cagagtgaag atttaccggc cccgctttta cccataatgc caatcacggg ttcgtgactg
      241 gtgagtttgc gcagatgttc caggatgtga cgtgaaagcg agtaaggcag ggaagaaagg
      301 ggtttttcaa ttgcctcaat ggcatcagac ggattcatac atgtattccc ggtgaaaaaa
      361 gacaaaaccc cgcgcaccgg aaaggtggcg ggggatccac ggagataata tctgtctgaa
      421 attgtttgga atcgatgggg atgttgcgta ttaacatgaa ggagggcagg agttatccgt
      481 tactgggtga tatccttcca tgaaaatacg cggttattgt taaacagcag gctgattact
      541 ccgtaaagga gaaaaatatt catcttgcag cctgattatc aggtgtgttt atgtttactg
      601 aggtgagtat ttttcagtat attgtcgggg cagtcagtgg gagatattaa atttacgttt
      661 agagttatct tgcttctgct gtgtattttt ataagagtgc gcacgtaatg atatctgttg
      721 ataccatgtg gttaagtgtt tcccttcctg gtcatgcgta atgattaaat gcttttgtga
      781 caccattctt ccggcatgaa tatttatcca gttccctttt ttatttgtgt agtgatactg
      841 tcggaatagc gttcagtaat gtgaagtgtc ctgttaaaaa tctttaataa atgtgtcatc
      901 ctggtcagga aatatctgct gagatattat tagttgctgg tgtttttact gagtaatagc
      961 tggcttttaa ttcctgaact gaaatactgt cgctcgtcag ggtttaatta aaactggatg
     1021 atgatgtgaa aatgaaggct ggtttttgtg tttgtcgaag aagtgttgtt tttatccatt
     1081 ctcatcattg gctggcgatt atacagaagt atttgaagaa aaaatggtgc attttatctg
     1141 tgccggcagg gagggggatt ttcctgatat gtcagtatta aatggttttt gttatttata
     1201 aaaatgtcat cctgtccata gaatattgta ctgagtactc agcattcagc gcaggaactg
     1261 tcatcttctt tattttattc ttcttcagga gggctgtttt ctgaaagcgt tactccgggg
     1321 aaactgttta tgtttattcc cggcaggaaa cccgtacctg caataccctg gatgatgctt
     1381 tttgctggta cgttgttgtg tgaaaattaa tcaacatgtg tgctccggag agcgcccgta
     1441 gacgcacaga ttcattctaa cattctttag cgacaaaata tcgagtggtt attattcaac
     1501 ctgaaaatcg acagaaattt ttatcctgta ttgattctgc tgctattttt gctattagta
     1561 gcaatagttg cggtacttta tgttctgtgt gttgtgaggt cattatgcca tactttcagt
     1621 atcctgatga gtttcccctg agctcgctgc ctccactcat tcgtgatgcc gttattgaag
     1681 ctcagcaaat cacgcaggct cccctggggc ttgtggcagc atctgccctg ggcgctgttt
     1741 cacttgtctg ccagaatctg attgatgtct gcagactgaa tacactacgg gggccggttt
     1801 ctcttttctt actgacgctt gctgagtccg gagaacgaaa aaccgcagtg gacaaattat
     1861 taatggagcc cctgtaccag caggagatgc tgttatattc cagacacaaa aatgaactga
     1921 cgacatggaa aaataaggaa gagcttttaa aggcgcagaa aaaagcattg ttgtcaaaac
     1981 tgaataaaga actccgcaaa ggcgcggatg agtcagaaac gctcagacaa ctggaagcgc
     2041 ttcagaaaaa cagaggggaa aaacctgtaa gatataaatt catttttaat gatgccacga
     2101 cagcagcaat taaagatcag ctttgtggtc agtggcgttc agtgggtatc atgtctgatg
     2161 aagccggtat aatatttgat gggtataccc tgtctgagct gccttttatt aataaaatgt
     2221 gggatggctc cgtattgtcg gtggacagaa agaatgaacc cgaacagatg attgaaaatg
     2281 ccagaatgac attatccctc atggtacaac ccggtctttt tgaccgatat atggagcgaa
     2341 aaggttctgt tgccagggac agtggttttc ttgcccgttg tctaatcagt aaacctgcca
     2401 ccacgcaggg gaaacgcttt attaacggag ctgtaacccc gggaggatca ttaacggcat
     2461 ttcatgaacg attaatggaa ctggccagag gcagtattga aaaatccagt aaggatgaac
     2521 gttattgtct gcatttttct cctgaagcgc aaaaaatatt tattgatcac tataatgttc
     2581 tggagcagga tttgtctcct tccggaccct taagccagtt tcgggggcat gtttcaaaaa
     2641 aaacagaaaa cattgcgaga attgctgcgt tattgcagta tttcagtcag ggtgaaggga
     2701 aaatctctgc cgatatcatg acgtctgctg t
//
LOCUS       DAZFRN010000314         2727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFRN010000314 DAZFRN010000000
VERSION     DAZFRN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.317"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..2640
                     /locus_tag="ACHKUB_005124"
     CDS             <1..2640
                     /locus_tag="ACHKUB_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6657990.1"
                     /translation="TTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAV
                     HREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAWG
                     KAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHY
                     DLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHR
                     VAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWL
                     TYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQ
                     HLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATD
                     PAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDE
                     RTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSR
                     KPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKL
                     QQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPE
                     YTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQ
                     QHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQ
                     LLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHA
                     GIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVG
                     IGAGVDASVMTLACRTWQECWVN"
BASE COUNT          661 a          705 c          865 g          496 t
ORIGIN      
        1 acgacaacgg atgcgacggg cagcacccgg cagatgacgt ggagccgcta cgggcagttg
       61 ctggcgttca ctgactgctc gggctaccag acccgctatg aatacgaccg cttcggccag
      121 atgacggcgg ttcaccgcga ggaaggtatc agcctttacc gccactatga caaccgtggc
      181 cggttaacct cggtgaaaga cgcacagggc cgtgaaacgc agtatgagta caacgccgca
      241 ggcgacctga ctgccgttat caccccggac ggcaaccgga gcgagacaca gtacgatgcg
      301 tggggaaagg cggtcagcac cacgcagggc gggctgacgc gcagtatgga gtacgatgct
      361 gccggacgtg tcatcagtct gactaacgag aacggcagcc acagcgactt cagctacgat
      421 gcgctggacc ggttggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
      481 gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcaccct ctggtactac
      541 gatgaatcgg accgtatcac tcaccgcacg gtgaacggcg aaccggcaga gcagtggcag
      601 tatgatgacc acggctggct gacagacatc agccacctga gcgaaggcca ccgtgtcgct
      661 gtccactacg gctacgacga taagggccgc ctgaccggcg aacggcagac ggtggagaac
      721 ccggagacgg gggaactgct gtggcagcat gagacaacac acgcctacaa cgaacagggg
      781 ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg agtggctgac ctacggcagc
      841 ggttatctgg cgggcatgaa gcttggcgac acgccgctgc tggagtacac gcgcgaccgc
      901 atgcaccgtg agacggtgcg cagctttggc agcatggcag gcagtaatgc cgcatataaa
      961 ctgaccagca catacacccc cgcaggccag ttacagagcc agcacctgaa cagcctggtg
     1021 tatgaccgtg actacgggtg gaatgacaac ggcgacctgg tgcgcatcag cggcccgcga
     1081 cagacgcggg aatacggcta cagcgccacg ggcaggctgg agagcgtgcg taccctcgca
     1141 ccggacctgg acatccgcat cccgtatgcc accgacccgg cgggaaaccg cctgccggac
     1201 ccggagctgc acccggacag taccctcacg gcgtggccgg ataaccgcat cgcagaggat
     1261 gcgcactatg tttaccacta tgatgaatac ggcagactga cggagaagac ggaccttatc
     1321 ccggcgggtg ttatccggac ggacgacgag cggacccacc actaccacta cgacagccag
     1381 caccgtctgg tgttctacac gcggatacag catggcgagc cactggtcga gagccgctac
     1441 ctctacgacc cgctgggacg gcgaatggcg aaacgggtat ggcggcggga gcgtgacctg
     1501 acggggtgga tgtcgctgtc gcgtaaaccg gagatgacgt ggtacgggtg ggacggcgac
     1561 cggctgacga cggtacagac cgacaccacg cgtatccaga cggtatatca gccggggagc
     1621 ttcgcgccgc tcatccgcat cgaaacagac aacggcgagc gggagaaagc gcagcgccgc
     1681 agcctggcgg agaagctgca gcaggaaggg agcgaggacg ggcacggcgt ggtatttccg
     1741 gctgaactgg tgcggctgct ggacaggctg gaggaagaaa tccgggcaga ccgcgtgagc
     1801 agtgaaagcc gggcgtggct tgcgcagtgc gggctgacgg tggagcaact ggccagacag
     1861 gtggagccgg aatacacacc ggcgcgaaaa gttcattttt accactgcga ccaccggggc
     1921 ctgccgctgg cgctcatcag cgaagacggc aatacggcgt ggcgcgggga gtatgatgaa
     1981 tggggcaacc agcttaatga agagaacccg tattacctgc accagccata ccgtctgccg
     2041 gggcagcagc atgatgagga atcagggctg tactataacc ggaaccggta ctatgacccg
     2101 ctacagggga ggtatattac acaagacccc attgggctgg cggggggatg gaacctgtat
     2161 aattacccac tgaatccgat aataaggatg gatcctttgg gtttgtataa tttatatcaa
     2221 ttattatatg atgtttggca tgatgattca tatggaacat catcaattga tattactggc
     2281 agtggagatc taatatcatt aggtggtcat gcaggacttg gcgttgcgtt tgctaaaaag
     2341 aaaggtgaaa tgttatctga tatttgtatt tatgctacag catgcggaca tgcaggaatt
     2401 ggtggtggga taaatgcggc tatcacatat tctgagacca agtctttacc tacatcggga
     2461 gtcagcaatt cagtaggtgt aacggttggc ggcggagttg gggggcattt tgcgtatact
     2521 tatgtagtgg atgttgataa tccagaatca tcgacagaat ctgttggtat cggtgcaggt
     2581 gttgacgctt cagttatgac tctggcttgt agaacgtggc aagaatgctg ggtcaattaa
     2641 caggaggaaa tatgaaaaaa tatatatttt tagttttatt tttattgtgt ggttgttctc
     2701 cagtggacat agagccagct agacaag
//
LOCUS       DAZFRN010000315         2725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFRN010000315 DAZFRN010000000
VERSION     DAZFRN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.318"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            3..1136
                     /locus_tag="ACHKUB_005125"
     CDS             3..1136
                     /locus_tag="ACHKUB_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6657991.1"
                     /translation="MLSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFR
                     QMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNL
                     HIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYN
                     ISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNR
                     NYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRP
                     DDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAV
                     VTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            complement(1177..2339)
                     /locus_tag="ACHKUB_005126"
     CDS             complement(join(1177..2078,2078..2339))
                     /locus_tag="ACHKUB_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFO6657992.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            2407..2725
                     /locus_tag="ACHKUB_005127"
                     /pseudo
     CDS             2407..2725
                     /locus_tag="ACHKUB_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT          687 a          613 c          655 g          770 t
ORIGIN      
        1 gtatacttag tttggctttt ctgagttgtt tgatttactt tgttaaaacc gtcattatca
       61 ttcagcaaat tattgagggg cgtttgacaa gcagtgttgt ccaaaatgac attgcaatct
      121 attatttgtt tcgtcagatg agtttgtgca tattaatatt tctggcattg gtgaataaag
      181 ttagtgaaaa cacaaaacag cgcaatttat tttccaaaaa aatgactttg tgcataagtt
      241 tgttttttgt ttttgggggg ccgatagttg ctcacatact ttctagtcat tatgagagct
      301 acaatttaca cattgccgag ttaaccaatg aaaatggtca ggtggtttgg aaagcctcat
      361 atgttaccat aatgattttc atgtggttaa ccttgctttc ggttaatcta tactttaatg
      421 gtttacgcta tgatatctgg aatggagtga cggtgattgc tttttgtgcc gtactgtaca
      481 atatctctct gttatttatg agtaggtata gcgtgtccac ttggtatatt agtcgcacta
      541 ttgaagtagt cagtaagtta actgttatgg taatatttat gtgccatata ttcagtgcgc
      601 tacgagtaac aaagaacatt gcacatcgcg atcccttaac caatatattt aacagaaatt
      661 atttttttaa tgaactgaca gttcaatcag catcagccaa aaaaacgcct tattgcgtca
      721 tgattatgga tatcgaccac ttcaaaaaag tcaacgacac ctgggggcat ccggttggcg
      781 atcaggtgat aaaaacagtg gtgaatatca ttgggaaaag catacgacca gatgaccttt
      841 tagcgcgcgt cggcggcgaa gagtttggcg tcttgctgac ggacatcgat actgaacgtg
      901 cgaaagcttt agcggaaagg attcgggaaa atgttgagcg tttaactggc gataatcctg
      961 aatacgctat accacaaaaa gtgacgatta gtattggcgc tgttgtgact caggaaaatg
     1021 cgttaaaccc aaatgagatc tatcgactgg ctgataacgc actttacgag gcgaaagaga
     1081 ccgggcgtaa taaggtggtt gtgagggatg tggtgaattt ttgtgagtca ccataaagcg
     1141 gcattttgat cctacccacg taatatggac acaggcctaa gcgaggttct tgttttcaaa
     1201 ttgttccgga ctgaggccgc cacaccaact gtgccgccgc caccgattgt aatcacattc
     1261 gatataatta aacaccgttg cccgcattat ttcccggctg ataaagtgtt ctccatggat
     1321 acattccact ttcagcgaat gaaagaagct ttccacgcag gcattatcgt agcagcaacc
     1381 ttttgcgctc atacttccac gcagattatg ccgcttcagt tgcgcctgat aatctgctga
     1441 acagtactgg cctccacggt ccgtgtgaac gataacgttc cggggcctct tacgccgcca
     1501 cagcgccatc tgcagggcat cgcaggccag ttgcgccgtc atgcgtggcg acattgacca
     1561 gccaataacg gcacgtgacc acaggtcaat gaccactgcc agatacagcc agccttcatc
     1621 tgtacgtaag tacgtgatgt ctcctgccca cttctggttc gggccactgg cgtaaaaatc
     1681 ctgctccaac agattttctg acacaggcag gccgtgtgcg cggtagctga ccgggctgaa
     1741 cttccgggag gcctttgccc tcagtccctg acggcgcagg cttgccgcca cggtttttac
     1801 gttaaagggg taaccctgag cacgcagttc atccgtcagg cgtggggcac cgtaacgctg
     1861 ttttgaccgg gtaaaagccg cgaggacaac gctgtcgcag tgttggcgga actgctgacg
     1921 cgtgcttatc cttgtccgcc gctgacacca cgtataccag ccgctgcggg ccacccggag
     1981 cacgcggcac attgctttga tgctgaactc agcctgatgt ttttcaataa agacatactt
     2041 catttcaggc gcttcgcgaa gtatgtcgcg gccttttgga ggatagccag ctcttcatcc
     2101 cgttctgcca gctggcgttt gagacgtgca atctcggtag acatctccag ttcacgttca
     2161 gaagacgtct gctgattttg ctgtttactg cgccagttgt agagctgtga ttcatacagg
     2221 ctgagttcac gggctgcggc agtaacaccg atgcgttcag caagcttcag ggcttcactg
     2281 cgaaattcag gcgaatgctg tttacggggt tttttactgg ttgatactgt ttttgtcatg
     2341 tgagtcacct ctgactgaga gtttactcac ttagccgcgt gtccactatt gctgggtaag
     2401 atcagtgcct ggcacacgcg tttgatggaa gacggcgttc aggcaatgcg cgattatctt
     2461 gccggactgg atatcgcttc gccagagcat caggttctga tgaacgtaac agcaaaaagc
     2521 gaggtcgctc cgtcaattat caaagaaaat ctctcattac acttaaccca tacggtgaaa
     2581 tggactgaat ctctcgatac atttctgaat atgccaaccc cggtagcttt ccttgaaata
     2641 agtaataagc cctatttggg gaatatgtta aatgattttg ctggcgtgga tcagcaacga
     2701 gttatgcatt gtcgtaaagc attta
//
LOCUS       DAZFRN010000316         2719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFRN010000316 DAZFRN010000000
VERSION     DAZFRN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.319"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(8..>2719)
                     /gene="rhsD"
                     /locus_tag="ACHKUB_005128"
     CDS             complement(8..>2719)
                     /gene="rhsD"
                     /locus_tag="ACHKUB_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO6657993.1"
                     /translation="TTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAV
                     HREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGNRSETQYDAWG
                     KAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSVFSYDALDRLVQQGGFDGRTQRYHY
                     DLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDGHGWLREISHLSEGHR
                     VAVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWL
                     TYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQ
                     HLNSLVYDRGYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATD
                     PAGNRLPDPELHPDSTLTVWPDNRIAKDAHYVYHYDEYGRLTEKTDRIPAGVIRTDDE
                     RTHHYHYDSLHRLVHYIRIQYEEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSR
                     KPEVTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKL
                     QQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPE
                     YTPARKAHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHVYQPYRLPGQ
                     QHDEESGLYYNRHRYYDPLQGRYITQDPMGLKGGWNLYQYPLNPLQQIDPMGLLQTWD
                     DARSGACTGGVCGVLSRIIGPSKFDSTADAALDALKETQNRSLCNDMEYSGIVCKDTN
                     GKYFASKAETDNLRKESYPLKRKCPTGTDRVAAYHTHGADSHGDYVDEFFSSSDKNLV
                     RSKDNNLEAFYLATPDGRFEALNNKGEYIFIRNSVPGLSSVCIPYHD"
BASE COUNT          482 a          854 c          701 g          682 t
ORIGIN      
        1 actaaaatta atcatgatac ggtatgcata ctgaactcaa tcccgggaca ctatttctga
       61 taaaaatata ttctccttta ttattaagcg cctcaaatcg tccatcaggt gttgcgagat
      121 aaaatgcttc aagattatta tctttacttc ttacaagatt tttatcgcta cttgaaaaaa
      181 attcatcaac ataatcgcca tgactatctg caccgtgagt atgataagca gcaactctat
      241 ctgtacctgt gggacatttt cttttcagag gatatgactc ctttcttaaa ttatcagttt
      301 ctgccttaga tgcaaaatat tttccattag tatctttaca gacaatacca gagtattcca
      361 tatcattaca tagagatcta ttctgcgttt ctttcaaagc atctaacgca gcatctgcag
      421 tactatcaaa tttacttggt cctattatac gtgaaagaac accacaaact ccccccgtac
      481 atgctccaga tctggcatca tcccaagtct gcaataatcc catagggtca atttgttgta
      541 gtggatttaa aggatactga tataaattcc atcccccttt caaccccatc gggtcctgag
      601 taatataccg cccctgcaac ggatcgtagt accggtgacg gttatagtac agccctgatt
      661 cctcatcatg ctgctgccct ggcagacggt acggctgata cacatgatgc gggttctcct
      721 cattaagctg gttgccccat tcatcatatt ccgcgctcca cgccgtattg ccgtcttcgc
      781 tgataagcgc cagcggcagt ccccggtggt cgcagtgata aagatgagct tttcgcgccg
      841 gtgtgtattc cggctccacc tgtctggcca gttgctccac cgtcagcccg cactgcgcaa
      901 gccacgcccg gctttcactg ctcacgcggt ctgcccggat ttcttcctcc agtctgtcca
      961 gcagccgcac cagttcagcc ggaaatacca cgccgtgccc gtcctcgctc ccttcctgct
     1021 gcagcttctc cgccaggctg cggcgctgcg ctttctcccg ctcgccgttg tctgtttcga
     1081 tgcggatgag cggcgcgaag ctccccggct gatataccgt ctggatacgc gtggtgtcgg
     1141 tctgtaccgt cgtcagccgg tcgccgtccc acccgtacca cgtcacctcc ggtttccgcg
     1201 acagcgacat ccatcccgtc aggtcacgct cgcgacgcca cacccgtttc cctgtccggc
     1261 gacccagcgg gtcgtaaagg tatcggctct cgaccagcgg ctcctcatat tggatacgga
     1321 tgtaatggac caggcggtgc aggctgtcgt aatggtagtg atgggtgcgc tcgtcgtccg
     1381 tccgtatcac acccgccggg atgcggtccg tcttctccgt cagtctgccg tattcatcgt
     1441 agtggtaaac atagtgcgca tccttcgcga tgcggttatc cggccacact gtgagggtac
     1501 tgtccgggtg cagctccggg tccggcagcc ggttgcccgc cgggtccgtg gcatacggga
     1561 tgcggatgtc caggtccggt gcgagggtgc gcacgctctc cagcctgccc gtggcactgt
     1621 agccgtattc ccgcgtctgt cgcgggccgc tgatgcgcac caggtcgccg ttgtcattcc
     1681 acccgtagcc acggtcatac accaggctgt tcaggtgctg gctctgtaac tggcctgcgg
     1741 gggtgtatgt gctggtcagt tcatatgcgg cattactgcc tgccctgctg ccgaagctgc
     1801 gcaccgtctc ccggtgcagc ctgtcccgcg tatactcgac cagcggcgtc ccgcccagct
     1861 tcatgcccgc caggtaaccg ctgccgtagg tcagccactc caccggcggc aggctgtccg
     1921 gcgtgacgcg gtttgccagc ccctgttcgt tgtatgcgtg tttcgtctca tgctgccaca
     1981 gcagttcccc cgtctccggg ttctccaccg tctgccgttc gcccgtcagg cggcccttat
     2041 cgtcgtagcc gtagtggaca gcgacacggt ggccttcgct caggtggctg atttctctca
     2101 gccagccgtg gccgtcatac tgccactgct ctgccgggtc gccgttcacc gtccggtgcg
     2161 tgatgcggtc cgacgcatcg tagtgccaga gggtgacaag cccctcatct tcactctgcg
     2221 tgagttttcc ggtcaggtca taatgataac gttgcgtccg cccgtcaaag ccgccctgct
     2281 gtaccagccg gtccagcgca tcgtaactga agacgctgtg gctgccgttc tcgttggtca
     2341 ggctgatgac acgtccggca gcatcgtact ccatactgcg cgtcagcccg ccctgcgtgg
     2401 tgctgaccgc ctttccccac gcatcgtact gtgtctcgct ccggttgccg tccggggtga
     2461 taacggcagt caggtcgcct gcggcgttgt attcataccg cgtttcacgg ccctgtgcgt
     2521 ctttcaccga ggttaaccgg ccacggttgt catagcggcg gtaaaggctg atgccttcct
     2581 cgcggtggac cgccgtcatc tggccgaagc ggtcgtattc ataacgggtc tggtagcccg
     2641 agcagtcggt gaacgccagc aactgcccgt agcggctcca ggtcatctgc cgggtgctgc
     2701 ccgtcgcatc tgttgtcgt
//
LOCUS       DAZFRN010000317         2664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFRN010000317 DAZFRN010000000
VERSION     DAZFRN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.320"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            148..1080
                     /locus_tag="ACHKUB_005129"
     CDS             148..1080
                     /locus_tag="ACHKUB_005129"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6657994.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(1121..2551)
                     /locus_tag="ACHKUB_005130"
     CDS             complement(1121..2551)
                     /locus_tag="ACHKUB_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6657995.1"
                     /translation="MIHKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT          713 a          574 c          544 g          833 t
ORIGIN      
        1 taaaaataaa gccatcccta ataattgaga taatgggatt ttttctaatg tggatagata
       61 tgaattattt ttctccttaa ggatcatccg ttatttgggt cgttttctgc taaaatctgc
      121 gccttgcgct tctttagcgc actgtctatg gctaaccttt tgaataaatt cattatgacg
      181 agaatactcg ctgcgataac ccttttgctg agtatcgtat tgaccatttt ggtcactatt
      241 ttttgttccg tcccgatcat cattgccggg attgtaaaac tattgctacc tgtgccagtc
      301 atttggcgaa aggtttcgcg tttttgtgat tttatgatgt attgctggtg tgaaggtctg
      361 gcggtattac tgcatctgaa cccacacctg caatgggaag ttcacgggct ggaagggtta
      421 agtaagaaga actggtatct gcttatttgt aaccaccgta gttgggcaga tattgtcgta
      481 ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat atttcctcaa gcaacagctg
      541 gcctgggtgc catttcttgg cctggcgtgc tgggcgctgg atatgccgtt tatgaagcgt
      601 tattcccgcg cttatttatt acgtcatccg gagcgacgtg gtaaagatgt tgaaaccact
      661 cggcgttctt gtgaaaagtt tcgcctgcat cccaccacca ttgtcaattt cgttgaaggc
      721 tcccgtttca cgcaagaaaa acatcagcaa acccactcca cttttcaaaa cttgttgcca
      781 ccaaaggcgg caggcattgc gatggcgcta aatgtactgg gtaaacaatt cgataaactg
      841 cttaatgtta cgctgtgtta tccggacaat aaccgtcagc cgttcttcga tatgttaagc
      901 ggtaaactga cgcgaattgt cgttcatgtg gatttacagc ctattgccga tgagttacat
      961 ggcgactaca tcaatgacaa aagctttaag cgccattttc agcaatggct gaactcactg
     1021 tggcaggaaa aagaccgact tctgacgtcg ttgatgtcat cacagcggca ggacaagtaa
     1081 attttgctcg cctctgaatg agcagaggcg agtgagtatt ttaatgaaca aaacgtccgg
     1141 cacgagacat aaattcttct tcgctcatct cctgtccgtt aaaaacaact ttacccggtg
     1201 tataacgcag ttgcagcgag gcggtattgt tgtcaatcgt gattaactga aacatctgac
     1261 ctaaggtcat catcccgctg atttgtttat cagcccgctt ttgcgctttt tccgcatcca
     1321 ttccttcaga taaattaagc tgttttgcgg tttctgtggc gacattaagc ggtaacttca
     1381 catcaaaatt gagcgattta atatctttgt ttgtggatga tgaagatttg gctgggtcgg
     1441 caatactgat atccagattg gcatttagtt cgccgagtgc gttcttccat tttaccggtt
     1501 gtttaatggt cggctcactt ttttgtaata acggtaaata ctctttgaac aatgcagcat
     1561 tcacttcttg cagagcaact tcatcgtttg ctaactcagg gtgtgcagca agctgcttct
     1621 gcattgcaat gttatactgg atgataaatt ggcgcaccgc tgagggatca atagattcag
     1681 caatcaatga ccactcacca ctaccgagtt gctgattccc ttttgttaac ttatcaagtt
     1741 cgtaggtcag attgacattg atataatctt tgtctttaac gcgatccagc atggtttttg
     1801 caacgaaagc atcgatttgg gcaacgtctt ccccgtgatt gataatgtta atctgattaa
     1861 atttcgactc gctttcacca accggaaatt tcttttcttc taatcgcgcc agagaatcaa
     1921 tggtaaaact ttttgccgtt actttgttca ttggtgataa ttgcagaaca atatcttcga
     1981 ctttgccttc aatatccagg ttagacaatg aactaccatc accattaaaa gtaaactggc
     2041 cctcaccaaa agaaaacttt ccatactctg caggggcaac atctaatgtc gtagagaatg
     2101 agccaccaaa cgcgaatcgc aatgtcgctt ccacgggcga tttatttttc gcggcgataa
     2161 atagcggttg tgtcagttca ttattcacta agttcacttt tgccgctgcc agtgctggga
     2221 tgacattacc atgcattaac attgtgatgg gcaacggacc gtgctcaaca tccacatcaa
     2281 atacaacttt ctggcctggc ttgatattga gatcgggtgc accgttatcg aatgtcattt
     2341 gcatttgaaa acgagaatta aaaaaacctt tgtcaaaatt tttataactt aacgtcatat
     2401 cgtaggcatg ttcacctttc tttttagcat cgttaaaatc tttaataaac ttttcgacac
     2461 caggctgaat ttgcgtacct gtgtaccatg tgccaccacc ccagattacg gctaacgcaa
     2521 caataacacc tgtagctgac ttatgtatca tataaatatt ccctgataaa acagcaaaac
     2581 tttaaatatc cgcttatgct gaaataatgt ccagcggcag gatgcattat cagaatgtat
     2641 ttatatttat taaataataa aaaa
//
LOCUS       DAZFRN010000318         2658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFRN010000318 DAZFRN010000000
VERSION     DAZFRN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.321"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            289..468
                     /locus_tag="ACHKUB_005131"
     CDS             289..468
                     /locus_tag="ACHKUB_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6657996.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            509..754
                     /locus_tag="ACHKUB_005132"
     CDS             509..754
                     /locus_tag="ACHKUB_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6657997.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLELRLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDGGEPTPDSDMMM"
     gene            831..1046
                     /locus_tag="ACHKUB_005133"
     CDS             831..1046
                     /locus_tag="ACHKUB_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6657998.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            1051..1584
                     /locus_tag="ACHKUB_005134"
     CDS             1051..1584
                     /locus_tag="ACHKUB_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6657999.1"
                     /translation="MSRKLRYGLSAAVLALIGAGASAPEILDQLLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALTWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1805..1918
                     /locus_tag="ACHKUB_005135"
     CDS             1805..1918
                     /locus_tag="ACHKUB_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658000.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIIAIAVLIWAIRWW"
     gene            1920..2414
                     /locus_tag="ACHKUB_005136"
     CDS             1920..2414
                     /locus_tag="ACHKUB_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6658001.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTVTRDYFTLRERLMTMQKQLEGAQEYIRTQCIKLSFYY
                     PEDI"
     gene            2411..2635
                     /locus_tag="ACHKUB_005137"
     CDS             2411..2635
                     /locus_tag="ACHKUB_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658002.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          676 a          600 c          777 g          605 t
ORIGIN      
        1 ccgttcacac tggtgaaatc tccggcatca ggggcggcgg acaaactgca actgacagac
       61 atatcaaagg cggcgacata tacgttgctg attgacagtg tgaaggtgga agtgaacaac
      121 gcggatgccg cggcatgata aaaaaggccg ccagcaatga aggaaaggga tgaactttgt
      181 ccactacatg aatgctggcg gctggtaaaa cgaccatgat gacaccgaag tctgcggtgt
      241 ttttctacaa aaataagtaa atcatgtcaa ctatcacggt gagaaaccat gacatttctg
      301 aaccagttaa tgctgtactt ctgtacggtg gtctgtgtgc tgtatcttct ttcgggtggg
      361 tacagggcag tgcgcgattg ctggcgcagg cagattgata aaagggccgc agagaaaatc
      421 agcgccagtc agtcagccgg aagcaaaccc gaagagccgc tcatttagcg gcaactttct
      481 taacaaccct tttcaacgag aaaatcccat gtcagaaatc acatccctgg tcactgcaga
      541 ggcagtgaag gaagtcctgc gctctgaaga agtcctgagc gcactgaaac agaaactccg
      601 ccagaacctt gagttgcgtc ttgatgcaga agtggatgcc attctggatg aactgctggg
      661 cgcaccggca gctccggagc cggaaggcat cgcgggtgag gggagtgctt cagatggcgg
      721 tgaacccaca cctgacagcg acatgatgat gtaagcatgc gcaagggacc atcggtgtgt
      781 gccggtggtc tttatattgt tgtgagcttc cggattgcgg gagacggggt atgtaccaga
      841 tggaaaaaat cacaacaggt gtgtcataca ccacgtcagc ggtgggaacg ggctactggt
      901 tcctgcagtt gctggacagg gtttccccgt ctcagtgggc ggcaataggt gtgctgggga
      961 gtctgctgtt tgggctgctg acatatctga cgaacctgta tttcaagatt aaagaagacc
     1021 gtcgtaaggc ggcgcgggga gagtaagctg atgagcagga aactccgcta tggtttatcg
     1081 gctgccgttc tggcgctgat tggtgcaggg gcttctgcgc ctgaaatcct cgaccagttg
     1141 cttgacgaaa aagaaggtaa ccacaccaca gcataccgtg atggtgcggg tatctggacc
     1201 atctgccgtg gagccacccg ggtggatggt aagcctgtga ttcctggcat gaagctgtcg
     1261 aaggggaaat gcgaccaggt taacgccatt gagcgtgata aagcgctgac gtgggtggag
     1321 aaaaacatca gagtgccgct gaccgaaccc cagaaagcgg ggatcgcgtc attctgtccg
     1381 tacaacattg gtcccggtaa gtgtttcccg tcgacgtttt acagacgaat taatgcaggt
     1441 gatcgcaggg gagcatgcga agcgattcgc tggtggatta aggacggtgg cagagactgc
     1501 cgtattcgct caaataactg ttacggtcag gtatcccgtc gtgaccagga gagcgcgctg
     1561 gcgtgctggg gaatcgacag ataagcagaa tattttgctg aaaaatgacg ttgaccaacg
     1621 cggacggata acacgaaatc ctgcgaactg gcaaaaccta agtgaataaa agtaaaaacc
     1681 ccgtttgttg gcagcaagcg gggttttgtg tttctgacct tggataaggc aagggagaaa
     1741 ttatggtgag ggaggtgctt tccctgtgag gaagtataaa agattctttc tgaggttgtc
     1801 cattatgaaa ggcattgaag tggagacgcc agccagtctg gatttaacaa gagcggcagc
     1861 ttttgccatt cgtattatag ccattgctgt tctgatctgg gcaatccgtt ggtggtgaaa
     1921 tgaaccgcgt tctgtgtgtg gtcatcattg ctctgctggt ggcctgtggt gcgcttagtc
     1981 tggggctgaa tcattaccgc gataacgcca tcacttacaa agcgcagcgc gataaaaaag
     2041 ccagagagct ggagctggca aacgcaacca ttactgatat gcaggtgcgc cagcgtgatg
     2101 ttgctgcgct cgatgcaaaa tactcgaggg aattagccga tgcgagagct gaaaatgaaa
     2161 ctcttcgcgc tgacgttgcc gctggtcgta agcgcctgcg tatcaacgcc acctgtccag
     2221 gtcccgtgcg tgaagccacc ggcaccgccc gcgtggataa tgcaaccggc ccccaactgg
     2281 cagacaccgt tacacgggat tatttcaccc tcagagagcg gctgatgacg atgcagaagc
     2341 aactggaagg ggcgcaggaa tatatccgta ctcagtgcat taagttgtct ttttattatc
     2401 cggaagatat atgaagaaat tactggtaac cgtaaagcct tttcagggaa caattccgtt
     2461 ccgtattttg cagcgtggtc gtgttcttgt tgaaggttcg ttcagtggta aatgtacgca
     2521 attacactcc cggacctttc aggtgaatgc cacgaatgaa gagctaaccg ttgagtgtac
     2581 gatgaatgcc gctaaatgcc gcatggtatc tgctgcatta cagccagtgt gttgagcgac
     2641 cttattatcc atgcgcgg
//
LOCUS       DAZFRN010000319         2652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFRN010000319 DAZFRN010000000
VERSION     DAZFRN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.322"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            7..1059
                     /gene="yhiM"
                     /locus_tag="ACHKUB_005138"
     CDS             7..1059
                     /gene="yhiM"
                     /locus_tag="ACHKUB_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO6658003.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1375..2577)
                     /gene="yhiN"
                     /locus_tag="ACHKUB_005139"
     CDS             complement(1375..2577)
                     /gene="yhiN"
                     /locus_tag="ACHKUB_005139"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO6658004.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT          620 a          675 c          627 g          730 t
ORIGIN      
        1 tttaaagtga acatatatat cgggtggctt ttcaaattaa tccctttgat tatgggatta
       61 atttgcatcg cgttaggtgg ctttgtgctg gaaagttcag ggcaaagcga gtatttcgtc
      121 gcgggtcatg tgctgatttc tctggcggcc atatgcctgg cattattcac taccgcattt
      181 attatcattt cgcagctcac gcgcggcgtt aatacgtttt acaatacatt gttccccatt
      241 attggctatg cggggtcaat tatcaccatg atatggggtt gggcactgtt agcaggcaat
      301 gatgtgatgg cagacgagtt tgtcgccggc catgttattt tcggcgttgg tatgattgcc
      361 gcctgtgtat cgacggtggc agcgtcatcc ggtcactttc tgctcattcc caaaaatgca
      421 gcggggagca agagcgatgg aacgccggta caggcttatt cttcattaat cggtaattgc
      481 ctcattgccg ttcccgtttt actcaccctg ctcggtttca tttggtctat tacgctgtta
      541 cgtagtgctg acataactcc gcattatgtc gcgggtcacg tattgcttgg gttaaccgca
      601 atctgtgcct gtctaattgg ccttgttgcc acaattgtcc atcaaacacg taatacgttt
      661 tcaactaaag aacactggct gtggtgttat tgggttattt ttctcggctc aatcacggta
      721 ctgcagggga tatacgtctt agtcagttcc gatgcaagcg cccgactggc tcccggcatt
      781 attcttattt gcctcggaat gatctgttac agcatattct caaaagtctg gctactggca
      841 ctggtatgga gacgtacctg ttcgttagcc aacagaatac cgatgattcc cgtcttcacc
      901 tgcctgtttt gccttttcct ggcatcgttt cttgcggaaa tggcgcagac cgacatggga
      961 tattttattc cttcgcgggt tttggtcggt ttaggagcgg tatgctttac gttgttctca
     1021 atcgtttcaa tattagaagc aggttctgct aaaaaataat tgcaacgtac cggataaaac
     1081 cagcgttgac catttgcgta acgctggttt ttcttaggca tcatgaaata acgcagcatt
     1141 aatgcatagt ggttaaggat aaaaaaacaa caaagttact gtttttttca acctgttcat
     1201 attttataaa gatctggcca ctgggtgagt tttcaatcac ctttccttcc accttatatt
     1261 aagcctggag ggtttcagtt cacgggctca ttagaaaata atcacaagat agccactatc
     1321 aaaattagct catttttaat gcggctctaa taattttcat ctttaggtaa caggtcagga
     1381 cgacttcgct gcaatcaaat cctgcgcaca agcccacgca ctcgaccacg cccactggaa
     1441 gttatagccc cccagccagc cggtaacgtc catcacttcg ccgatgaagt acagcccagg
     1501 cactttgcgc gcttccatcg tccgtgaaga gagttcgttg gtgtccacgc cgccgagcgt
     1561 cacttcggca gtgcgatagc cttcagtgcc gttgggttgt acgcgccagt cggtcaatgt
     1621 gctaatcagt gcctgttggt cacgcacgtt gagctgtttt agcgaaacat ccgggatttg
     1681 cccgagttgc tgtaagcgtt caaccaaccg cttcggtaga tgaaccgcca gtgtgttttt
     1741 caggctctga ttcggatgtg cgttacgctg ctcattcagg aaggtttcga gatccacatc
     1801 cggtagcaga ttgatactga caaattcacc cggttgccag tagctggaaa tctgcaacac
     1861 cgccggtcca gacaagccgc gatgggtgaa gagtaagttc tcacggaaaa cgatgccgtt
     1921 ttcagcggta atcacggaag gcaccgccac gcccgccagc acctgtaact cttcgagcaa
     1981 cggtttatgc agagtgaatg gcaccagacc cgcgcgggtc ggcagcacgt tgaggccaaa
     2041 ttgttcggca atcttataac caaacggcga cgcgcccagc cccggcattg acagcccacc
     2101 ggtcgcgatg accagctttt cgcaaccgac agtcatgccg ttcagttcaa gcgtgaagcc
     2161 tgtttcatcc ttcgccacac tcagcacttc gctacgcaat ctgaaggtta cattgccctt
     2221 ctcgcactca tccaccagca tgtcgacaat ctgctgcgcg gagtcatcgc agaagagttg
     2281 tcctaacgtt ttctcgtgcc aggcgatgcc gtgtttattg accagatcaa tgaaatccca
     2341 ctgggtaaaa cgcgcgagtg cagacttaca aaaatgcgga ttctggctca gataagcgcc
     2401 tggttcgaca taaaggttgg taaagttgca gcgcccaccg ccagacataa ggattttgcg
     2461 ccctggtttt ttaccattat cgatcagcag aacccggcgt cctgcctgac ctgccagcgc
     2521 agaacagaac atacccgccg caccagcgcc tataataatg gcatcaaacc tttccacgtt
     2581 gcgctcctct tagaaaaaac gggcgtgaat tgtaaagatt cctcagtggt cgcaccagca
     2641 tcaatattac ta
//
LOCUS       DAZFRN010000320         2619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFRN010000320 DAZFRN010000000
VERSION     DAZFRN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.323"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            94..1566
                     /locus_tag="ACHKUB_005140"
     CDS             94..1566
                     /locus_tag="ACHKUB_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6658005.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            1570..2400
                     /locus_tag="ACHKUB_005141"
     CDS             1570..2400
                     /locus_tag="ACHKUB_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6658006.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            2446..>2619
                     /locus_tag="ACHKUB_005142"
     CDS             2446..>2619
                     /locus_tag="ACHKUB_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HFO6658007.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESN"
BASE COUNT          661 a          432 c          646 g          880 t
ORIGIN      
        1 ggtcttattg gtgcaactct ttgggaccgg gattgtatta cttaaagtaa tactccagtg
       61 tattataaat ccgctattat tgtgaggttt aatatggact cctgtattca gaaaaagcag
      121 gtttggtata agcatctttc acccagacag tggaagatat tcggtgcggc atggacaggt
      181 tatttacttg atggttttga tttcgttctg atatctctgg ttcttacaga ggttcagcat
      241 gaattcggct tgacgacaat agaagcggca agccttattt ccgctgcttt tatttcccga
      301 tggtttgggg ggcttgctat tggcgcttta tcggacaaga tggggcgcag gatggcaatg
      361 gttttaagta tagtgctatt ctctctcgga acccttgcct gtggtctggc tcctggatat
      421 gcagtgatgt ttattgctcg cattgttatt gggcttggta tggcaggtga atatggctca
      481 agtgtaacat atgttatcga aagctggcct gttcatctca gaaacaaggc aagtggtttt
      541 ttaatttctg gattttctat tgggggaggt ttagctgcac aggtttatag cattgtagta
      601 ccactatggg gctggcgttc tcttttcttt gtcggcatgc ttccaatatt atttgctttt
      661 tatcttcgca agaatcttcc ggagtctgat gactggcaaa aaaggcaaca agagaataaa
      721 cccgtcagaa caatggtgga tattctgtac agagaaaaga ataaatacat aaatattctt
      781 ctgtcttgta ttgcatttgc atgtctatat gtttgttttt caggcgtcac tgcaaacgct
      841 gctttaatca cagttatggc attgtgctgt gcggctgttt ttatcagttt tatatatcag
      901 ggtatgggga aaagatggcc aacaggcatt atgttgatgc ttgtggttat gttctgtttc
      961 ctctacggat ggccactgca ggcttttctt cctacatggc ttaaggttga tatgcagtat
     1021 tcaccagaaa ctgttgctct catatttatg ctggcaggtt ttggctctgc agctggaagt
     1081 tgtataggtg gttttatggg ggactggctt ggaacccgaa aagcatatgt tatatcactt
     1141 cttatcggtc agcttgttat tatccccgtt tttcttgtgg accgggacta tgtatggtta
     1201 cttggccttc tgatctttac tcagcaggtt tttggtcagg ggattggtgc tctggtgccg
     1261 aaaataatca gtggttattt taatgtggag cagcgtgcgg caggactggg gtttatttat
     1321 aatgtaggtt cacttggtgg agcatgtgcg cctattcttg gtgcagttgt tgcgtctcat
     1381 acaagtcttg gtacagcaat gtgttctctt gcttttatct taacatttgt tgttcttgtc
     1441 cttattggtt ttgatatgcc atcacgtgta cagagatgga tccatccaga agctgcgctt
     1501 gaatatgata ctgtcgatgg aaaacctttt tatggagcaa gaaaaaagaa tgttgcagga
     1561 gaataaagga tgctatcagg gaataaaaaa atgattcatt acagggagaa tatatattct
     1621 cttgataatt atgagcgtta cataactgac aattcgctat ttgagaatac tgacgatggc
     1681 ataatatata caggtattgt gccccttatt acagttgttg agcctgttgt atctaatcaa
     1741 catagtgtgc tgattatacc tggaggtgga tacaggagga ttgtttatga aagagaggga
     1801 cttgatgtag gcagatggat agcaggtcag ggatatacgg catttattct ttcttacaga
     1861 atgccctccg ttaatggtta ttattccttt gcggatgcga agaatgccct gaactttatt
     1921 tcttactggg ccggaatgtc ggagagaaca tttaagagtg ttggatgtat ggggttttcg
     1981 gcaggtggtc atcttgctgg atatattact catctccatt atgaggaaga gacttctgtg
     2041 cgtcctgact ggatggctct gatatatcca gttatatctc ttgacaggag tattgctcat
     2101 cagggatgcc ggaaacgtct tctggatgca ggtgaaagtg aatattcagc cagtcttgaa
     2161 aatagtgtta cagagtatat gccaccagtc tttattgtgc atgcaacaga tgataaggat
     2221 gttccagtta ttcactctct gagatatttt aactctatcc gggataaggg acatcatgca
     2281 gaagctcatt tttttgataa gggagggcat ggatttggtg tatccggaaa aaatggatat
     2341 ccgatagaaa aatggcaaag cctgcttctg gaatggcttg cggaacataa gtttcattga
     2401 gaaatcagat tacaagtgat aaatatattt aatgatgagg catttatgaa aaaatattat
     2461 ccactattaa ctgtgattct tcttggttta cctttgtgct ctcaggcatt aactcttaat
     2521 ctacgtggag gatacagaag cgccagtcac gcttatgaaa cgttgattca ggttagtcag
     2581 gggtggaata atggttggtg ggcaagtttt gagtcaaat
//
LOCUS       DAZFRN010000321         2574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFRN010000321 DAZFRN010000000
VERSION     DAZFRN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.324"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..648
                     /locus_tag="ACHKUB_005143"
     CDS             <1..648
                     /locus_tag="ACHKUB_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6658008.1"
                     /translation="ALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFF
                     EASHKLTEHLLEQGYKNIGFIGAHMENRLQRQQLNGWHKAMLSHYQNTDLVITMPDAA
                     SLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDS
                     CDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1403..2026
                     /locus_tag="ACHKUB_005144"
     CDS             1403..2026
                     /locus_tag="ACHKUB_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6658009.1"
                     /translation="MHIRIDYLADKNCFTELNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYNLKA
                     SLSGGPDTFRYHLSHRIRRVVANENTSSPYQLIAREVKAPRQRLKYALETGLQVSALD
                     GLFWFGSQRIAADILRLRKYGMRIATASVDVYDNITGTTRHIPVYYS"
     gene            2248..>2574
                     /locus_tag="ACHKUB_005145"
     CDS             2248..>2574
                     /locus_tag="ACHKUB_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO6658010.1"
                     /translation="MQHRQKNILTKTSLLSRALSVPCCDMFRRGSPWICYLSLSVFSG
                     CFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTE
                     GPCFTVS"
BASE COUNT          664 a          614 c          638 g          658 t
ORIGIN      
        1 gcgttagtta tttttggttc tcagttggct gataaaactc accagatact cgagaaaaca
       61 aatataccta ccattaacgt tgtcggttcc cctttcagtc cggctcaaat taccatcgaa
      121 acagcatttt ttgaagcatc acataaactg accgaacatc tgctggagca gggttataag
      181 aatatcggat ttatcggtgc gcatatggaa aaccgcctgc aacgtcagca actgaacggc
      241 tggcataaag caatgctgag ccattaccag aacactgatc ttgttatcac aatgccggat
      301 gccgcaagtc tgcaattggg gcgttacgca ttaaacgaga tattactgcg acaaccagag
      361 ctggatgctg tgatttgcag ccacgaagag attgcgctgg gtatcatgtt tgagtgtcag
      421 cggcgtttgt taaaaatccc tggaaatatc gctgtagctt gtcttgacgg ttcagacagt
      481 tgcgatcaaa cacacccaac cttgacatcc atccgtattg actacaaaaa aatggggata
      541 gagacgggta agttactgat tggactattg aataataatc atgatgaatc agaagagtcg
      601 cgtatcgtgc aattcaacta ccagatagaa ttacgtcaga gtacatgagc ctaaaagggc
      661 actattgcaa agttagctat tgtggtaggg atagccgttt ccggctattc cccgcgcaga
      721 tccggatgtg tgctgctaac gcatccattc ctacttcagg tatttggcgt agaagcggat
      781 gctaaaaact tgccgtaaaa ccgcaatatc agcccgcagt gtcgctctcc agattatcca
      841 ttcctatatt gatgacaacg acaggtaaga ctgtgactca ccaaacttgg atgtttttct
      901 gagcttaaag ctcgttgcgt gaataaggcg agagtcagcg aatctcatcg aggcccgcag
      961 caccggctgc aatagggata tagagcagca atctaccgtc aacgtcaaaa acacggaagc
     1021 cttgcaaacg ggatgcgttc atataaacaa catggagcag caacaatacg atgcctgttg
     1081 ttgctcagtc aggacctttt ctgccatgcg cgagccagcg tcacgattga tgaagaatgt
     1141 tctcaactga ttaatttact gcaacagacg tggctggtaa taagcgctgg cggacaatgc
     1201 agggtgaaag gctgttttcg ggttgtccgg cctgatacat cagagcaata cgttgcgtta
     1261 aaaacggctg ttcagtctct gatgccacgg ggtattgcaa caatcatccg gtgatggacg
     1321 ggagaaatat cgactcttcc gttagttttt acagactgat aaagttttgc cagctgaatc
     1381 tttatacgga atgctcttca gtatgcatat acgaattgac tatctggcgg ataaaaactg
     1441 ctttacagaa ctgaatgaat ctccacgcct tcgccggcag tggcaggatg ttctggagga
     1501 gtgccggcag acagaggccg ggccagaaga acggctgcgt attgctctgc tgaatgtgga
     1561 ttacgtcacc agttttgaac tgccttttcg cttgttgctt actcgtacac cacaactgat
     1621 tgccgcgctg cgtgaagaat gggacatcag ccagaaaaat gtggtgttca acgataaacg
     1681 ctttggctgc gtgtacaacc tgaaggccag cctttcgggt gggccggata cattccggta
     1741 tcatctgtct caccgtattc ggcgggtggt tgcgaatgaa aacacatcat cgccatacca
     1801 actgattgcc cgggaagtga aagcgccccg tcaacggctg aagtatgcgc tggagacagg
     1861 attgcaggtg agcgcactgg acggcctgtt ctggttcggc agccagcgca ttgcggcaga
     1921 tatcctgagg ctgagaaagt acgggatgcg gattgcgacg gcttctgtgg acgtgtatga
     1981 caacataacc ggaacaactc gtcatatccc tgtatattac tcctgacgca aaatagtctg
     2041 tgggggccgg agcatgaccg gtaaccggaa cagggttttg ccctcgtcac agccaaaaat
     2101 acgtccttta tcacatttct tcgatttttc ctgatatttt accgggttac tgttcaaaaa
     2161 tgcttctctt ctgatgtaaa tttctttact gacacttttt gacatgaatt tacactggca
     2221 tcacacaggc cagaatacgg ggagaacatg cagcaccggc agaaaaacat tctgacgaaa
     2281 acgtcccttt tatcccgtgc gttgtctgtc ccctgttgtg atatgttccg gcgcggctct
     2341 ccgtggatat gctatctctc cctctccgtt ttttctggtt gtttcatccc cgcattttcg
     2401 tctccggcag ccatgctgtc tccgggtgac cgcagtgcaa ttcagcagca acagcagcag
     2461 ttgctggatg aaaaccagcg tcagcgtgat gcgctggagc gcagtgcgcc gctgaccatc
     2521 acgccgtctc cggaaacgtc tgccggtact gaaggtccct gctttacggt gtca
//
LOCUS       DAZFRN010000322         2481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFRN010000322 DAZFRN010000000
VERSION     DAZFRN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.325"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            52..1257
                     /gene="yedE"
                     /locus_tag="ACHKUB_005146"
     CDS             52..1257
                     /gene="yedE"
                     /locus_tag="ACHKUB_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO6658011.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1254..1487
                     /gene="yedF"
                     /locus_tag="ACHKUB_005147"
     CDS             1254..1487
                     /gene="yedF"
                     /locus_tag="ACHKUB_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO6658012.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1594..2265
                     /gene="yedK"
                     /locus_tag="ACHKUB_005148"
     CDS             1594..2265
                     /gene="yedK"
                     /locus_tag="ACHKUB_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO6658013.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(2305..>2481)
                     /locus_tag="ACHKUB_005149"
     CDS             complement(2305..>2481)
                     /locus_tag="ACHKUB_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6658014.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
BASE COUNT          522 a          643 c          675 g          641 t
ORIGIN      
        1 gtatgattcg cgactcttgt ttcaaacctt ctgagttcag aggctacatt catgtcatgg
       61 cagcaattca aacacgcctg gttgattaaa ttctgggcac ccatccctgc ggtcatcgcg
      121 gcgggtattc tctccactta ctattttggc attactggca ccttttgggc tgtcacgggt
      181 gaatttaccc gttggggcgg ccagctcctg cagctgtttg gcgtccatgc tgaagagtgg
      241 ggttatttta aaattatcca tctggaagga tcgccattaa cccgcatcga cgggatgatg
      301 atcctcggca tgtttggcgg ctgctttgcc gcagcgctgt gggccaacaa tgtcaaactg
      361 cgcatgccac gcagccgtat ccgcattatg caggccatta ttggcggtat tatcgctggt
      421 tttggcgcgc gtctggcaat gggctgtaac ctggcggcgt tctttaccgg tattcctcag
      481 ttctcgctgc atgcctggtt ctttgccatc gccactgcca ttggttcatg gtttggcgcg
      541 cgctttaccc tgctgcccat cttccgtatt cccgtgaaaa tgcagaaagt ttctgccgcc
      601 tcaccgctga cgcaaaaacc ggatcaggcg cggcgtcgtt ttcgtctcgg gatgctggtc
      661 tttttcggca tgctgggctg ggcgctgctc acggcgatga accaacccaa actggggctg
      721 gcaatgctgt ttggcgtcgg ctttggttta ctgattgaac gtgcgcaaat ctgctttact
      781 tcagcgttcc gcgatatgtg gatcaccgga cgtacccata tggcgaaagc aatcattatt
      841 ggtatggcgg tgagtgccat cgggatcttc agttacgtac agttaggcgt tgaacccaaa
      901 atcatgtggg cgggaccaaa cgcggtaatt ggtggtttac tgtttggttt tggcatcgtg
      961 ctggcaggcg gctgcgaaac cggctggatg taccgcgcgg tagaaggcca ggtgcactac
     1021 tggtgggtcg gtctgggcaa cgtgatcggc tcaacgattc tggcgtatta ctgggatgat
     1081 ttcgctccgg cgctggccac cgactgggac aaaatcaacc tgctgaaaac ctttggcccg
     1141 atgggtggcc tgctggtgac atatttgctg ttgtttactg cgctgatgtt gattatcggc
     1201 tgggaaaaac gcttcttccg ccgtgcggca ccgcagactg ctaaggagat cgcatgaaaa
     1261 atatcgttcc tgattaccgc cttgatatgg tgggtgaacc ctgcccttat ccggcagtcg
     1321 caactcttga ggcgatgccg cagttaaaaa aaggggaaat cctggaagtg gtgagcgact
     1381 gtccacagtc gatcaataat attccactgg atgcgcgtaa tcacggctat acggtgctgg
     1441 atattcagca agacgggccg accattcgtt atttaattca gaagtaatcc ctcattcccg
     1501 ctgtggccca cagcgggaac atcgttgcct ctgacacata gattttaccc gccccctctc
     1561 ctattctttt cagtatgatc tcaggaggtt tctatgtgtg gacgctttgc ccaatcccaa
     1621 acgcgtgaag attaccttgc gcttctcgcg gaagatattg aacgcgatat tccctacgat
     1681 cccgaaccca ttggcagata caacgtcgcg ccgggaacca aagttcttct gctcagtgaa
     1741 cgtgatgaac accttcatct ggatccggtt ttctggggat atgctcccgg atggtgggat
     1801 aaaccgccgc tgattaacgc ccgcgtagaa actgcggcca ccagtcgtat gtttaaaccg
     1861 ctctggcaac atggtcgggc aatctgtttt gccgatggct ggtttgagtg gaaaaaagaa
     1921 ggcgacaaaa aacagcctta ttttatctat cgcgctgatg gacaacctgt ttttatggcc
     1981 gcgataggta gcacaccgtt tgagcgtggt gacgaagccg aaggattttt aattgtcact
     2041 gctgcagcag accagggtct ggtagacatt catgaccgcc gcccactggt actgtcgcca
     2101 gaagctgcgc gggaatggat gcggcaagag attggcggta aagaagcatc agaaattgct
     2161 accaacggct gtgtgccagc aaaccagttc acctggcacc cggtatcgcg cgcggtaggt
     2221 aatgtcaaaa accagggcgc ggagttaatt caacctgttt gttgacatcc tccacgccct
     2281 gaaggacgag gatccctgct acgttcaggc tgtcgcctga atcatttcgg tgggttcctg
     2341 cttcaacggg cggcctgact gcaccatctc tccacaggca agaacggcgt gccccgccgc
     2401 taaaatgtta cgagcgccat ttacatcggc gttcgctgta tatccacata cctggcatct
     2461 gaatttactt tgtgacaggc g
//
LOCUS       DAZFRN010000323         2470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFRN010000323 DAZFRN010000000
VERSION     DAZFRN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.326"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            1..66
                     /locus_tag="ACHKUB_005150"
                     /pseudo
     CDS             1..66
                     /locus_tag="ACHKUB_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            576..2354
                     /locus_tag="ACHKUB_005151"
     CDS             576..2354
                     /locus_tag="ACHKUB_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFO6658015.1"
                     /translation="MRNARTILGIIRKRGAEGKSLDQIYRMLFNPELYIAAYAKIGSN
                     KGAMTKGVTDETVDGMSHKKIHDIISLLRNERYKFSPARRIYIKKKNGKMRPLGLPTW
                     SDKLLQEVMRMILEAYYEPQFSQFSHGFRPQRGCHTALTVIAKTWTASTWFIEGDIKG
                     CFDNIDHEILLHILREQIKDNRFIRLIEKLLYSGYMEEWKCHKTWSGTPQGGIISPLL
                     SNIYLNKLDQYVEHVLLPKYNKGKSRARNREYEVQNGIEHYNRKRGRLDISRAARKKK
                     QTLPSKDPFDPHYRRLRYVRYADDFLLGFSGPKSEAEEIKKEITLFLQNNLSLEVSQE
                     KTLITHGRTDKARFLGYDIQVGQCDTFRNRKKRRSVNGDIRLLVPADVVREHSKRYFL
                     KGRVRRRPELLEESDYTIVHNFQTRFRGLANYYQLACDRSKKLSRLKGIMEHSLMLTL
                     ANKHKTWVSRIYQKYGTRCMMTNGKSYKCLQIRIDRVNKAPLTATWGGISLARNKNYR
                     NIVLVDEKDLLFYGRVERLKAILASECAICGSTQNIQVHHIRALKDLAKRGRTPTASQ
                     RRMAARRRKTLVVCHQCHVAIHQGKL"
BASE COUNT          755 a          520 c          622 g          573 t
ORIGIN      
        1 catgacggta cctccagcta ccagatgctg ccgggttact tccggttcgt ctgccagaac
       61 gggtgcgccg tgtcggcgtg gtaagggatt cctgattcaa tcaggagtgg tcggaatgaa
      121 ataccactga gtcaacgcct tacctgaggc tgaaaggtca aggggacaat agcatggcgg
      181 taaaccggag agggccagaa aacgtgtatg gctgaactgt tccgggagac tcgcgcaata
      241 tggtgaaagc tacactgcct gaaccctaat aaccaagcgg gataaggcac tccccgtcac
      301 taccacgttt aagagtgatg ataaccggat cagacgaaat gccgttggtc gtcccaatca
      361 ttccccggtt aagccgtacc cagtgagggt accaaggtta tgagtagggc acctaagttg
      421 cgctgttacg ttccttaccg tgtaaacacg ggattgtcta aggggcgcga gccctagggc
      481 aacggagcca ccgtagtagt ccgggtccgg gaaagccgga tacatggcga agggtggcag
      541 gttacaacga tgtttaaaat tgagaggtac gcgagatgcg aaacgccaga acaatattag
      601 gcatcatccg caagagaggg gctgaaggta aatcgctgga tcagatttat cggatgcttt
      661 ttaacccgga gttatatatt gctgcctacg caaaaatcgg tagcaacaaa ggggcaatga
      721 caaaaggagt taccgacgaa accgttgatg gaatgtcgca caaaaagatc catgacatta
      781 tcagtcttct gcgtaatgag agatataagt tctccccagc cagaaggatc tatattaaga
      841 aaaagaacgg gaaaatgcgt cctcttggcc tgccaacatg gagtgataag ctgctccagg
      901 aggtcatgcg tatgatcctt gaggcctatt acgagcctca gttcagccag ttctctcacg
      961 gttttcgccc acagcgtggt tgtcatacag cactgacagt catcgccaaa acatggacgg
     1021 cttccacatg gtttattgaa ggggatatta aagggtgttt cgataatatt gatcacgaaa
     1081 tattgctgca tatacttcgg gaacaaatta aagataatcg attcatccgg ttaattgaga
     1141 aactgcttta ctccggatat atggaggaat ggaaatgcca taaaacctgg agtggaacac
     1201 ctcagggtgg aataataagc ccgttgctct ctaatatata cctgaataaa cttgaccagt
     1261 acgtggaaca cgtgttattg cctaaatata acaagggtaa atccagagca aggaatcgag
     1321 agtatgaagt tcagaatggt attgaacatt acaatcgtaa aagggggaga ctggatatat
     1381 ccagagcggc acgtaagaag aaacaaacgt tgccgtcaaa agaccctttt gacccgcatt
     1441 atcggcggtt gcgatatgtg agatatgcag acgacttcct gctcggattc tctggcccaa
     1501 aatcagaagc tgaggaaatc aaaaaggaaa taaccctgtt tcttcagaat aatctgagtc
     1561 ttgaagtttc tcaggagaaa acgcttatta cccatggaag aacagataaa gcccgttttc
     1621 ttggatacga tattcaggtt ggtcaatgtg atacgttccg taacagaaag aaaagacgca
     1681 gtgtcaatgg cgacatccgg ctacttgttc ccgccgatgt agtgagagaa cacagcaaac
     1741 gttactttct taaaggacga gtaaggagac gaccggaatt actggaggag agtgattaca
     1801 ctatcgttca taatttccag acccgttttc gtggtttagc taactattat caactagcct
     1861 gtgacagaag taaaaaatta tctcgtctga aaggcatcat ggaacattcc ctaatgttaa
     1921 ctctggctaa caaacataaa acgtgggtta gccggatcta tcaaaaatat ggaacacgat
     1981 gcatgatgac taatggtaaa tcttacaaat gtctacagat aagaatagat agagttaata
     2041 aagcaccatt gactgcaaca tggggcggaa tatctctggc ccgtaacaaa aattaccgaa
     2101 atattgtgtt agttgatgag aaagacttgc tcttttatgg gcgcgttgag cgactgaaag
     2161 ccatactggc ttctgaatgt gcaatatgtg gctcaaccca aaatatacag gttcaccaca
     2221 ttcgggcact gaaagatctt gcgaaacggg gaaggacgcc cacagcatca caaagaagga
     2281 tggcagcaag gcgacgtaag acactggttg tatgtcacca atgccatgtc gcgattcatc
     2341 agggaaaact ctaattacct tgcggatttg ttgtaactgg agagccggat gcgatgaaag
     2401 tcgcacgtcc ggttcggaga ggggctgacg gaaaagtgcc aatacagcaa ctcgtcggca
     2461 gcctacccta
//
LOCUS       DAZFRN010000324         2375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFRN010000324 DAZFRN010000000
VERSION     DAZFRN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.327"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..219)
                     /locus_tag="ACHKUB_005152"
     CDS             complement(<1..219)
                     /locus_tag="ACHKUB_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6658016.1"
                     /translation="MSELSYLEKLLDGAEVEWKTLGKVLKRTKGTKITAGQMKALHKD
                     NAPLKIFAGGKTVAFVDFKDIPEKDINRE"
     gene            complement(216..1772)
                     /locus_tag="ACHKUB_005153"
     CDS             complement(216..1772)
                     /locus_tag="ACHKUB_005153"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO6658017.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSGYMEAGDDSICYAKLDDNVITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLNLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IRLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKETNNNILTDAHIEQIMQVFSSKEDVAHL
                     AKSVTFEAIVANDYNLSVSSYVEAKDTREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            2038..>2375
                     /locus_tag="ACHKUB_005154"
     CDS             2038..>2375
                     /locus_tag="ACHKUB_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO6658018.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILP"
BASE COUNT          582 a          585 c          541 g          667 t
ORIGIN      
        1 ctctctatta atatctttct ctggtatatc tttaaagtca acaaaggcta ctgtttttcc
       61 tcctgcaaaa attttaagcg gcgcattatc tttgtgaaga gctttcatct gcccagcggt
      121 tatttttgtg cctttagttc gtttaagcac tttccctagt gttttccact caacctcagc
      181 cccatccagc aatttttcca gataactcaa ctcgctcatt tctgcacctc gcagccttca
      241 atttcagcca caatcgcatc aatatcttta cgcaactggt cgattttgct gaccgtggtt
      301 ttcagctcag cattcagctc agcaatattg ataatttcac gagtatcttt cgcttccaca
      361 tagctgctca ccgacaggtt atagtcattc gctacaatgg cttcaaacgt gacagatttc
      421 gccagatggg caacatcttc cttgctggaa aatacctgca taatctgttc gatatgagca
      481 tcggtcagaa tgttgttgtt agtctctttt ttgaacagtt cgctggcatc aataaactga
      541 actctggtat ccgttttatg tttagacagc accagtatgt ttacggcaat ggtggtgcca
      601 aagaacaggt tcggtgccag tgaaatcacg gtttcgacat agttattgtc gaccagatac
      661 tgacggattt tctgctctgc gccgccacgg taaaaaatac ccgggaagca gacaatcgca
      721 gcgcgacctc tggccgaaag atagttcagc gcatgtaata caaacgcaaa gtcagctttg
      781 gatttggggg ccagaacgcc agccggggca aaacgctcat cgttaatcag tgtcgggtca
      841 tcgctgccag tccatttcac cgaatacggc gggttagaaa cgatggcatc aaacggtttt
      901 tcatctctga agtgcggctc agtcagcgta ttgcccagcc tgatatcaaa cttgtcgtag
      961 ttgatgttgt gcaaaaacat gttcatacgc gccaggttat aggtcgtatg gttgatttcc
     1021 tgaccaaaaa agccttcttc gatgatatgg tcatcaaact gctttttcgc ctgcaacaac
     1081 agcgaaccgg aacccgctgc cgggtcgtag attttgttaa cgtgggtctg cccgtgcata
     1141 gccagttgtg caatcagctt agagacgtgc tgtggtgtaa agaactcgcc gcctgactta
     1201 ccggcatttg cggcatagtt agaaataagg aattcatagg catcgccaaa caggtcaatc
     1261 tgatgtttgt tgaagtcacc aaggtttaac ccttcaaccc ctttcagaac agcagccagg
     1321 cgggcatttt tatccttaac ggtgttaccc aggcggttac tggtggtatc gaaatcagca
     1381 aacaaaccct tgatgtcagc ttctgaagga taaccgtaag cagaactttc gatagcaacg
     1441 aagatgctgt ttaaatctgc attcagtctg tcattggtat ttgctttcgc agctacgttg
     1501 cagaaaagct gactggggta gatgaagtag cctttagtcc tgatggcatc gtctttaatg
     1561 tcatcagtaa ttacgttgtc atccagtttt gcataacaga tgctgtcatc accggcttcc
     1621 atatacccgg agaaattttc gctgataaaa cggtagaaaa gcgcgcccag aacgtattgc
     1681 ttaaaatccc atccatcgac cgaacctctg acatcgttag caatttgcca tatctggcgg
     1741 tgcagttctg ctctttgttg aatacttgtc atttttaacc tgtttttcct ggtctggaac
     1801 tgaatctccg ggggcaatac tacagcaatc ttgtggttca ggcagccccg gagcaagtca
     1861 gcagatattg tgagatacac caggttattc atgacaggga ggaggaacgg aacactgcct
     1921 ttcttttaca gtgcaccggg tacattcaaa ataatttcag acagatatta tctccgtgga
     1981 tcccccgcca cctttccggt gcgcggggtt ttgtcttttt tcaccgggaa tacatgtatg
     2041 aatccgtctg atgccattga ggcaattgaa aaaccccttt cttccctgcc ttactcgctt
     2101 tcacgtcaca tcctggaaca tctgcgcaaa ctcacccgtc acgaacccgt gattggcatt
     2161 atgggtaaaa gcggggccgg taaatcctca ctctgtaatg cactgtttca gggggaggtc
     2221 accccggtca gtgatgttca cgccggcacc cgggaagtac ggcgcttccg tctgagtggc
     2281 cacggtcaca gcatggttat cactgacctg cccggggtgg gcgagagccg ggacagggat
     2341 gcagagtatg aggccctgta ccgtgacatt ctgcc
//
LOCUS       DAZFRN010000325         2356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFRN010000325 DAZFRN010000000
VERSION     DAZFRN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.328"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..493
                     /locus_tag="ACHKUB_005155"
     CDS             <1..493
                     /locus_tag="ACHKUB_005155"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6658019.1"
                     /translation="APQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVADPNL
                     DELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGKGLAK
                     KLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCEVRML
                     REL"
     gene            complement(539..955)
                     /gene="rraB"
                     /locus_tag="ACHKUB_005156"
     CDS             complement(539..955)
                     /gene="rraB"
                     /locus_tag="ACHKUB_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO6658020.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1117..2121
                     /gene="argF"
                     /locus_tag="ACHKUB_005157"
     CDS             1117..2121
                     /gene="argF"
                     /locus_tag="ACHKUB_005157"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO6658021.1"
                     /translation="MSRFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRTLAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          597 a          564 c          571 g          624 t
ORIGIN      
        1 tgcgccgcaa tcacctgtaa tgcgtcgcct gacgcagcag gataatcccg ccatcgcccg
       61 cgtcattcgt caggtatccg ccgaatacgg tcttaccgct gataaaggct acaccgtcgc
      121 cgatccgaac ctggacgagt tgtatcaagt gtatagtcag ccgggtcatg catattgggt
      181 ggttgagtac gatggtgaag tggtcggcgg cggtgggatt gcgccattaa ccgggagtga
      241 gtcggacatt tgcgaactgc agaagatgta ttttcttccg gctattcgcg gtaaagggct
      301 ggcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct tcaaacgctg
      361 ctatctggaa acgaccgctt ttttgaaaga agcgattggt ttgtatgaac atttaggttt
      421 tcaacatatc gactatgcac ttggctgcac gggccatgta gattgtgaag tgcggatgct
      481 acgcgaactc taaaaaaatg cccgctagtg tgtgctatcg ggcattttaa tcaattaatt
      541 agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgccg tcttcgccgt
      601 taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg
      661 tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac tcgctgagga
      721 tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga tcggtcactt
      781 cgtaaccgag tttaaacgct tcaactgctg ctttttccag ggtttccaga tcgtctgcgg
      841 aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc agtaattctt
      901 caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt gccatggctc
      961 attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat tgcctccacc
     1021 tttgtgacaa agatttatgc tttagacttg caaatgaata atcatccata taaattgaat
     1081 tttaattcat tgaggcgtta gccacaggag ggatctatgt ccaggtttta tcataagcat
     1141 ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca gttagccgcg
     1201 aaactgaaag ccgataagaa aagcggcaaa gaagaagcca aactcactgg taaaaacatc
     1261 gcgctcatct tcgaaaaaga ctcgacccgt acccgatgct ctttcgaagt tgccgcatat
     1321 gaccagggtg ctcgcgttac ttatctcggc ccaagcggca gtcagattgg tcataaagag
     1381 tcgattaaag acactgcccg cgtgctgggt cgcatgtatg acggtattca gtatcgcggc
     1441 tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt atggaacggc
     1501 ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat gcaggagcat
     1561 ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc acgtaacaac
     1621 atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg tctggtcgcg
     1681 ccacaggcat gctggccgga agctgcgctg gttacggaat gccgcaccct ggcacagcaa
     1741 aatggcggga atattacgct gacggaagat gtagccaaag gagttgaagg tgctgacttt
     1801 atctataccg atgtatgggt gtcgatgggg gaagcaaaag agaaatgggc agaacggatt
     1861 gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg taacccggag
     1921 gtcaaattcc tccactgcct gcccgcgttt catgacgacc agacgacgct tggtaagaaa
     1981 atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt cttcgaatct
     2041 gccgccagca ttgtttttga tcaggcggaa aaccgcatgc ataccatcaa agcggtgatg
     2101 gtcgcgacgc tcagtaaata attgatatcg ctataaagat gccgggaaat acaaaaacat
     2161 cccggcatca cattatttta tgcagtaata gttcaattat tccatccata tttttctatt
     2221 gctatacgta aatcttccgg aggattttca tcattcttgt tctaattttt aattcattaa
     2281 ccatcttttc ttcaaaaatc ttaacgacat aacccggatt gttttttaca atttcatttg
     2341 ttttttcttc attacc
//
LOCUS       DAZFRN010000326         2334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFRN010000326 DAZFRN010000000
VERSION     DAZFRN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.329"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1105
                     /locus_tag="ACHKUB_005158"
     CDS             <1..1105
                     /locus_tag="ACHKUB_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HFO6658022.1"
                     /translation="IRLTPTDILCHRNYFYQKCFTNDGKKLIFAAEFDCNRNYYLMDL
                     VTQTAKQLTEGAGDNTFGGFLSPDDNYLFYVKNEKHLQRVDLTTLEEVTIYTVPDNWV
                     GYGTWVANTDCTEIVGIEISKTDWTPLSDWKIFLEFYHKNPRCRLITIDLETGNAEVI
                     LDRNTWLGHPIFRPNNNDTIAFCHEGPHDLVDARMWMINRDGTNERKVHEHQPGESCT
                     HEFWIPDGSALAYVSYMKGQQQRYIRKYNPDTDEDSLVMEMPACSHLMSNHNGILYVG
                     DGSGSPVDVKDTCSYKIENDPWIYILSSKNKKIHKLVKHNSSWRVQKGDRQVTHPHPS
                     FSPDNKYVLYSCDAEGEPALYMAKIPEEILTQQ"
     gene            1135..1935
                     /locus_tag="ACHKUB_005159"
     CDS             1135..1935
                     /locus_tag="ACHKUB_005159"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO6658023.1"
                     /translation="MDFINMFSLKGKNALVTGASYGIGFEIAKALSNAGATIIFNDVI
                     EENIKKGLAAYKENNINAHGYLFDITDEQSVKENIKKIESDVGIIDILVNNAGIIQRK
                     PMLETTAADYRKIIDIDLTGQFIMAKAVLPSMIKKGHGKIINICSMMSELGRETVVGY
                     ASAKGGLKMLTKNICSEFGEKNIQCNGIGPGYIATPQTAPLREIQPDGSRHPFDQFII
                     SKTPAGRWGKPDDLAGAAIFLASDASNFINGHILYVDGGILAYIGKQP"
     gene            1990..2313
                     /locus_tag="ACHKUB_005160"
     CDS             1990..2313
                     /locus_tag="ACHKUB_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO6658024.1"
                     /translation="MFVFNEDTAIEDLGNGVKRKVLAHGGNMMAVEVHFSKGAIGSLH
                     HHQHEQLTYVLSGIFEFTIGDEVRTVKTGDTLYKQPNIVHGCKCLEKGVLLDIFTPQR
                     QDFLK"
BASE COUNT          747 a          397 c          497 g          693 t
ORIGIN      
        1 tattcgatta acgccaacag atattctttg ccatcgcaat tatttttatc aaaaatgttt
       61 tacaaatgat ggtaaaaaat tgattttcgc tgctgagttt gattgtaacc ggaattatta
      121 tcttatggat ttggtaactc agacagcgaa acaactcact gaaggagctg gagataacac
      181 ctttggtggc ttcctgtccc ctgatgataa ttatcttttt tatgtgaaga atgaaaaaca
      241 tttgcagcgt gttgatttga ccacattaga agaagttacc atatacacag taccggataa
      301 ttgggtgggg tacggaacat gggttgctaa tactgattgt acggaaatcg tcggaatcga
      361 aatcagtaaa actgactgga ccccgcttag cgactggaaa atatttctgg aattttatca
      421 taagaatcca cgatgccgtt taattacgat tgatctggaa accggaaacg ctgaagtgat
      481 attggatcgt aatacttggt tagggcatcc aatttttcgt ccgaataata acgatactat
      541 cgccttttgc cacgaagggc cccatgacct ggtggatgca agaatgtgga tgattaaccg
      601 tgatgggaca aatgagcgta aagtgcatga gcaccaacct ggtgaaagtt gtacacacga
      661 gttctggatc cctgatggat ctgcactggc ttatgtttcc tatatgaaag gacagcaaca
      721 gcgttatatt cgcaaatata atcctgatac tgatgaagat tcattagtga tggaaatgcc
      781 ggcatgttcc catttaatga gtaaccataa tggtattctc tatgttggtg atggttcagg
      841 tagtccggtt gatgttaaag atacctgcag ttataaaatt gaaaatgatc cctggattta
      901 cattcttagc agtaaaaata aaaaaataca taaattggtg aaacataata gttcctggag
      961 ggtacagaaa ggagaccggc aggtaacgca cccgcatcca tcattttctc cagataataa
     1021 gtacgtttta tattcttgcg atgccgaagg tgaaccagca ctatatatgg ctaaaattcc
     1081 tgaggaaatc ttaacccagc aataactgac aaatttttca ctattggagt ttttatggat
     1141 ttcatcaata tgttctctct taaaggtaaa aatgctttag tcactggagc atcatatggt
     1201 attggttttg agatcgctaa agcactaagt aacgctggtg caacgatcat ttttaatgat
     1261 gtaatagaag aaaacattaa aaaaggcctg gcagcatata aagagaacaa catcaacgca
     1321 catggttatt tatttgatat tacagatgaa cagtctgtga aggaaaatat caaaaaaata
     1381 gagagtgacg taggcattat tgatattctg gtaaataatg cagggattat tcagcgtaaa
     1441 cctatgctag aaactacagc agctgattat cgcaaaataa tagatattga tctgacaggt
     1501 cagtttatta tggctaaggc ggtattgcct tcaatgatta aaaaaggaca tggcaagatc
     1561 attaatatct gttccatgat gagcgaattg ggtcgcgaaa ccgttgtagg ctacgcttcg
     1621 gcaaaaggcg gcttaaaaat gctgactaaa aatatctgtt ctgagtttgg tgaaaaaaat
     1681 attcagtgta atggtattgg tccaggctac attgctacac ctcaaacagc accgttacgt
     1741 gaaattcaac cagatggcag ccgccatcca tttgatcagt ttattatatc aaaaacgcca
     1801 gctggacgct ggggtaaacc agatgatctt gctggtgcag ctatattttt agcatcggat
     1861 gcttcaaact ttatcaatgg tcatatttta tatgtcgatg gtggcattct ggcctacatt
     1921 ggcaaacagc cttagttata ttcagtcata actgtatgta ctactttaaa tattttcaga
     1981 gaggtggtga tgtttgtatt taatgaagat acagcaatag aagatttagg caacggggtc
     2041 aaaagaaaag ttttggctca tggtggcaat atgatggcag tagaggtcca tttctctaag
     2101 ggggctatcg gctctttaca tcaccaccaa catgaacagt tgacgtatgt cttatcaggt
     2161 atttttgaat ttaccatcgg cgatgaagtg cgtacagtaa aaactggtga tactttatat
     2221 aagcagccca acattgtgca cgggtgcaaa tgtctagaaa aaggagtttt gcttgatata
     2281 tttacacctc aacgtcagga ttttttaaag taaataccca gtattaatat gaac
//
LOCUS       DAZFRN010000327         2276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFRN010000327 DAZFRN010000000
VERSION     DAZFRN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.330"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..358
                     /locus_tag="ACHKUB_005161"
     CDS             <1..358
                     /locus_tag="ACHKUB_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6658025.1"
                     /translation="ELASQPGACVAQIARENGVNDNVIFKWLRLWQNEGRVSRRLPVT
                     TSSDTGIELLPVEITPGEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTVLIRELTGRGR"
     gene            355..705
                     /gene="tnpB"
                     /locus_tag="ACHKUB_005162"
     CDS             355..705
                     /gene="tnpB"
                     /locus_tag="ACHKUB_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6658026.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGELVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNSLTML"
     gene            736..>2276
                     /locus_tag="ACHKUB_005163"
     CDS             736..>2276
                     /locus_tag="ACHKUB_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO6658027.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDEQLSLVEETEAFLRSAL
                     ARAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEISAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNTFKLHTDDTPVKVLA
                     PGRKKAKTGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLRPFRGILQADAF
                     NGYDRLFSAEREGGALTEAGCWAHARRKIHDVYISTKSATAEEALKLIGELYVIEHEI
                     RGLPVSERLAVRQMQSKALLTSLHKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFC
                     DNGLAEVDNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LSHIL"
BASE COUNT          565 a          580 c          649 g          482 t
ORIGIN      
        1 ggaactggca tcacaacccg gagcttgtgt tgcacagatt gcacgtgaaa atggcgtcaa
       61 tgataatgtt attttcaaat ggctcaggct ctggcagaac gaagggcgtg tttcgcggcg
      121 tcttccggta acgacctctt ctgacactgg cattgaatta ttacctgtag agataacgcc
      181 gggtgaacag aaagaacctg tggcggccat tgcgccgtct ttatccactt ccactcagac
      241 cagagtcagt gccagctcct gcaaggtgga gttccgtcac ggtaacatga cgctggaaaa
      301 tccatcgcca gagctgctca cggtgttgat ccgtgaactg accgggaggg gaagatgatc
      361 tcactcccat caggtacccg catctggctc gttgccggcg ttaccgatat gcgtaaatcc
      421 ttcaacggac tgggagaact ggtacaacat gtgctgaatg ataatccctt ctccggtcac
      481 ctgtttatct tccgtggccg acggggtgac accgttaaaa ttctttgggc cgatgctgat
      541 ggtctgtgcc tgttcaccaa acgcctggag gaaggccagt ttatctggcc tgcggtacgt
      601 gacggcaagg tatccattac ccgttcgcaa ctggcaatgc tcctcgataa gctggactgg
      661 cgtcagccaa aaacatcccg ccttaactca ctgacaatgt tgtaaaaaac tcctgaccgc
      721 attataaaaa cggtcatgag tcagaaatac ctcattcgca tcgcagagct ggaaaggttg
      781 ctctctgagc aggctgaagc cctccgtcag aaagacgagc aactgagtct ggttgaagag
      841 acggaggcct tcctgcgctc tgcactggca cgtgccgaag aaaagatcga agaagatgaa
      901 cgggaaatag aacatctgcg ggctcagata gaaaaactgc gccggatgct gttcggtacc
      961 cgttctgaaa aactgcgtcg cgaggttgaa caggctgagg ccctgctgaa gcaacgcgaa
     1021 caggacagcg atcgttacag tgggcgtgag gatgacccgc aggttccccg ccagctgcga
     1081 cagtcacgcc atcgtcgtcc gttaccggag catctgcccc gcgaaataaa ccgcctggag
     1141 cctgaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctggg ggaaatcagt
     1201 gcagaacaac tggaactggt gagcagcgcc ctgaaagtga tccgcacaga acgggtaaaa
     1261 aaagcctgta caaaatgtga ctgtattgtt gaagcaccgg cgccgtcccg cccgatagag
     1321 cgtggtatcg cgggccccgg attacttgcc cgcgtgttaa cgggaaaata ctgcgaacac
     1381 ctgccactgt atcgtcagag tgaaatcttt gcccgccagg gcgtcgaact gagccgggcc
     1441 ttactctcca actgggttga tgcgtgttgc cagttaatga cgccgctgaa tgatgctctg
     1501 taccgttacg tgatgaatac cttcaagctt cacactgacg acacaccggt aaaggtactg
     1561 gcaccgggca ggaagaaggc gaaaacagga tatatctgga cgtatgtccg ggatgaccgg
     1621 aatgccggtt cgccagaacc cccggcggtc tggttcgcct actcaccgga tcatcagggt
     1681 aaacatccgg agcagcacct tcgtcccttc cggggtatcc tgcaggcaga tgcgtttaat
     1741 ggttacgatc ggctgttcag tgccgaacgt gaaggcggcg cgttgacgga agcaggatgc
     1801 tgggctcatg cgcgacgcaa aatccacgat gtatatatca gtaccaaaag cgcgacagcg
     1861 gaagaagccc tgaaactaat cggtgaactg tacgtcatcg agcacgaaat acgcgggttg
     1921 ccggtgtctg aacgcctggc ggtcaggcaa atgcagagta aagcgctact gacgtccctg
     1981 cataagctga tgcaggagaa agaacacaca ttatcgaaaa aatgtcgtct gagagatgcg
     2041 ttccggtata tcaggaagca ctgggttgcg ttgtgcaact tctgtgataa cggtctggcg
     2101 gaagtggata ataatgccgc ggaaagagcg cttcgtgcag tctgtctcgg aaagaaaaac
     2161 tttatgttct tcggcagcga tcacggcggc gagcgtggag cactgttgta cgggctgatc
     2221 ggcacctgcc gtctgaacgg tatcgatccg gaagcgtatc tgagccatat tctgag
//
LOCUS       DAZFRN010000328         2226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFRN010000328 DAZFRN010000000
VERSION     DAZFRN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.331"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            5..1159
                     /gene="ompC"
                     /locus_tag="ACHKUB_005164"
     CDS             5..1159
                     /gene="ompC"
                     /locus_tag="ACHKUB_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6658028.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPASGKKVGEKAVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
     gene            1369..2067
                     /locus_tag="ACHKUB_005165"
     CDS             1369..2067
                     /locus_tag="ACHKUB_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6658029.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            2078..>2226
                     /locus_tag="ACHKUB_005166"
     CDS             2078..>2226
                     /locus_tag="ACHKUB_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6658030.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRIT"
BASE COUNT          520 a          456 c          661 g          589 t
ORIGIN      
        1 aatcatgaaa aaaatgacag tggcactttc tgctgtagca gtggcagtga tgtttgctgc
       61 gggggcgcag gcagcagaag tttataataa agatggtaat aaactggatc tttacgggcg
      121 tgcaaccgct ctgcattact tctcggatga taaaggtaat gacggagacc agacttatgc
      181 tcgtctcggc tttaaaggcg aaacgcagat taatgatcaa ctgaccggat ttggtcagtg
      241 ggaataccag ttctctggta ataaaacgga atctgaaggt tccgcgggaa ataaaacccg
      301 tctggcattt gcaggtctga gatttgcaga tgttggtacc attgattacg gacgtaacta
      361 tggtattgct tacgatgtcg gatcatatac tgacgtactg cctgagtttg gtggtgatgg
      421 ctggacgcag accgataact ttatgacggc ccgaacttcc ggtgtcctga cttaccgtaa
      481 tacagacttc ttcggactgg ttgatggtct gaattttgcg gcgcagtatc agggcaaaaa
      541 tgagcgtgat gaccttcaga aggctaatgg cgacgggtat ggtttctcgg ccagctatga
      601 gtttgatggt tttggttttg tcgctgcgta taccaagtca gatcgtactg ataagcaggt
      661 tcaggggttg aatggtactg cagatgttct tgatcctgca tcaggtaaaa aggttggaga
      721 aaaagcagtt gactcaggta gtgtggcaaa aggtaaacat gctgaattct ggggaactgg
      781 tcttaaatat gatgccaata acctgtatct ggcagcagtg tattctgaaa cccagaatat
      841 gacgaccttt ggtgatcaag gtgttgcgga taaagctcag aatatcgaag ccgttgttca
      901 gtatcagttt gattttggtc tgcgcccgtc ccttgcctac cttcagtccc gcggacagga
      961 tgttatggtt ggtggtgtga accatggcga tcaggatctg gttaaatata ttgatgtcgg
     1021 tgcgacttat tactttaaca agaatatgtc cacctatgtt gattataaaa ttaacctgat
     1081 tgatgaaagt gaatttaccc ggaaagccgg tgttgcgaca gataatatcg ttgctgtggg
     1141 tatgacttat cagttctgat tattgctgat aagttaataa atacgggccg tctgccctta
     1201 caggcggcct gtatcaatga aaacacagtt ttcattggtc actgcgatca gcaattgcca
     1261 tctggcatgt gctgatttaa ctttctgtta ttacctttat tggttttatt ttaaattgga
     1321 cttttattgt tcggggcgcg tctgcgcccc ttttttatgg gcggatatat gcaggatatt
     1381 cacgaagaaa gcctgaacga gtcggttaag tcagagcagt caccgcgggt ggtgctctgg
     1441 gaaatcgacc tgacggtgca gggcggtgag cggtattttt tctgcaatga gctgaatgaa
     1501 aaaggggagc cggtgacctg gcaggggcgt gaatatcagg cgtacccgat tgagggcagc
     1561 ggctttgaga tgaacggaaa gggcagcagt gcccgcccgt cgctgacggt gtccaatctt
     1621 ttcggccttg tcaccgggat ggcggaggat ttgcagagcc tggtgggtgc cacggtggtc
     1681 cgccgccggg tgtatgcgcg ttttcttgat gcggtgaact ttgtggcggg gaatccggag
     1741 gcagacccgg agcaggagct gacggaccgg tgggtggtgg agcagttatc agcgctgacg
     1801 gccatgacgg cctcgtttgt gctggcgaca ccgacggaga cggacggggc gctgtttccc
     1861 ggtcgcatca tgctggcgaa cacctgtatg tgggattacc ggggcgatga gtgcgggtat
     1921 aacggtccgg cagtggcgga tgagttcgac aaccctacca cggatatccg gaaggacaga
     1981 tgcagtaaat gcatgcgcgg gtgtgagatg cgcggcatgg cggtcaattt tggcggtttc
     2041 ctttccatca ataaactttc gcagtaaatc ccggtttatg acacagactg aatcagcgat
     2101 tctggtgcat gcccggcggt gtgcgccagc ggagtcgtgc ggcttcgtga taagcacccc
     2161 ggagggcgaa cggtaccagc cctgcgtgaa tatctccgca gagccggagg cgtattttcg
     2221 tattac
//
LOCUS       DAZFRN010000329         2159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFRN010000329 DAZFRN010000000
VERSION     DAZFRN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.332"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            156..2141
                     /gene="yjcS"
                     /locus_tag="ACHKUB_005167"
     CDS             156..2141
                     /gene="yjcS"
                     /locus_tag="ACHKUB_005167"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO6658031.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          569 a          573 c          528 g          489 t
ORIGIN      
        1 tccctcttca gcacaaatgc ctaataaaat ggctcgttcc gctcacactt ccacttatta
       61 aagtcgcaat ttcccaatta tgataacgcc agctttaaat gacgtatttt gcatggatta
      121 catatttacg acacaaatga taaatggaga tttatatgaa taactctcgg ttattccgtt
      181 tgagcaggat tgttattgcg ttaactgctg ccagcggcat gatggtaaat accgcttacg
      241 caacagatga agcgaaagcc gccactcaat atacccaaca ggttaatcag aattacgcca
      301 aatcattacc gtttagcgat cgtcaggatt ttgacgacgc tcagcgtgga tttatcgctc
      361 cgctgctgga tgaaggtatt ctgcgcgatg ctaatggcaa accatattat cgcggggaag
      421 attataaatt tgatatcaat gcgcccgcgc ccgaaaccgt taaccctagc ctgtggcgtc
      481 agtcgcagtt aaacggtatt tccggcctgt ttaaagtcac cgacagaatg taccaggttc
      541 gcggtcagga tatctccaac atcaccttta tcgaaggtga taccgggatt attgtcatcg
      601 acccgctggt gacgccaccg agcgcaaaag ccgcccttga cctttacttc caaaatcgcc
      661 cgcaaaaacc gattgttgcg gttatttata cccacagcca taccgaccac tatggcggcg
      721 tgaaaggcat tatctctgaa gctgatgtga aatccggtaa agtgcaggtt atcgcccctg
      781 ctggctttat ggacgaagcc atcagcgaaa acgtactggc gggtaatatt atgagccgcc
      841 gtgcacttta ctcctacggc ctgctgctgg cgcataaccc tcagggtaac atcggcaatg
      901 gtcttggcgt aacgctggca tcgggctacc cgagcatcat cgcaccgaac aaaaccatca
      961 ccaaaaccgg tgagaagatg attatcgacg gcctggagtt tgacttcctg atgaccccag
     1021 gtagcgaagc gccagccgaa atgcacttct atattccggc cctgaaagcc ctgtgtaccg
     1081 ccgagaacgc cacgcatacc ctgcacaact tctacactct gcgcggcgcg aaaacccgcg
     1141 acaccagcaa gtggaccgag tatctgaacg aaacgctgga tatgtggggt aacgacgcgg
     1201 aagtcctgtt tatgccgcac acctggccgg tctggggcaa taagcatatc aatgattata
     1261 ttggtaaata tcgcgatact atcaagtaca ttcacgacca gaccctgcac ctggcgaacc
     1321 agggctacac catgaatgaa atcggcgaca tgattaaact gccgcctgca cttgccaata
     1381 actgggccag ccgtggctat tacggttctg tcagccacaa cgcccgcgcg gtgtataact
     1441 tctatcttgg ctattacgac ggtaacccgg ctaacctgca tccgtatggt caggtggaga
     1501 tgggtaaacg ttacgtgcag gcgctgggcg gttctgcccg tgtcatcaac ctggcgcaag
     1561 aagcgaacaa gcaaggtgat taccgctggt cggcagaact gctgaaacag gtgattgccg
     1621 ccaacccggg tgaccaggtc gcgaagaatc tgcaagcgaa taactttgaa cagctgggct
     1681 atcaggccga gtccgccacc tggcgcggtt tctacctgac cggcgcgaaa gagctgcgcg
     1741 aaggggtgca taagttcagc cacggcacca ccggttcccc ggacaccatt cgcgggatgt
     1801 cggtcgaaat gctgttcgac tttatgtccg ttcgcctcga tagcgcgaaa gccgcgggta
     1861 aaaatatcag cctgaacttc aatatgagca atggcgataa cctcaacctg acgctgaacg
     1921 atagcgtgct taactaccgt aaaacactgc aatcccaagc tgacgcctct ttctacatca
     1981 gccgtgaaga tctgcacgcc gtgctgaccg ggcaagccaa aatggcggat ctggtaaaag
     2041 cgaagaaagc caaaattatt ggcaatggcg cgaaactgga agaaattatc gcctgtctgg
     2101 ataatttcga tttgtgggtg aatatcgtaa ccccaaatta atgtcactcg cttttgggg
//
LOCUS       DAZFRN010000330         2144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFRN010000330 DAZFRN010000000
VERSION     DAZFRN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.333"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>2144
                     /locus_tag="ACHKUB_005168"
     CDS             <1..>2144
                     /locus_tag="ACHKUB_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6658032.1"
                     /translation="ADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKVWD
                     VLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDR
                     HTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIK
                     TELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREV
                     TLPETGTSTVNLINGSGKPVRVDITAQPAPDRIQVSILPDGVATYGVWGLSLPSLRRR
                     LFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPLSGSLNSVIPPAVQHLTVEV
                     SASDGQYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLVTSAITADTEHRFSGLPLGE
                     YTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYFQITAVPVLAVYDPTVQYE
                     FWFSEKRITDTAQVETSARYLGTGSQWSVSGPHIRPGKDFWFYVRSVNLVGKSAFVEA
                     SGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQT
                     VSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQP
                     LSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSL
                     TPEGRLTARNADISGHISANSGTLNNVVIAEN"
BASE COUNT          482 a          584 c          684 g          394 t
ORIGIN      
        1 gcggacgtgg acaagtgggc actgtatgcc atcgggcagt actgtgacca gacggtcccg
       61 gatggtttcg ggggaacaga gccgcggatg actttcaatg cgtacctgtc acagcagcgt
      121 aaggtctggg atgtcctggg ggatttctgc tcggcgatgc gctgtatgcc ggtatggaac
      181 ggtcagacgc tgacgttcgt tcaggaccgt ccgtcggatg tggtgtggcc gtacacgaac
      241 agcgatgtgg tggtggatga taacggcgtg gggttccgct acagcttcag tgccctgaag
      301 gaccggcaca cggcggtgga ggtgaattac accgacccgc agaacggctg gcagacttcc
      361 actgaactgg tggaagaccc ggacgccatc ctgcgctacg ggcgcaatct gctgaagatg
      421 gatgcgtttg gctgtaccag ccgcggtcag gcccaccgtg ccggactgtg ggtgataaag
      481 acagaactgc tggaaacgca gacggtggat ttcacgctcg ggtcacaggg gctgcggcac
      541 acgcccggtg acatcattga aatctgtgat aacgactatg ccgggaccct gaccggcgga
      601 cgtatcctgt ccatcgatgc cgccagccgt accctgacgc tggaccgtga ggtgacactg
      661 ccggaaacag ggacatcgac ggtgaacctg attaacggca gcggtaagcc ggtgcgcgtg
      721 gacatcactg cacaacccgc ccctgaccgg atacaggtca gcattctgcc tgatggcgtg
      781 gcgacatacg gtgtgtgggg actctccctg ccgtcactgc gtcgtcgcct gttccgctgt
      841 gtttccatcc gggaaaacac ggacggcacc tttgccatca cggcagtgca gcacgtaccg
      901 gaaaaagaag ccatcgtgga taacggggcc cgctttgagc cgctctccgg ttcactgaac
      961 agcgtcatcc cgccggcagt gcagcacctc acggtggagg tgagtgcctc agacggccag
     1021 tatctggcgc tggcgaaatg ggacacgccg cgggtggtga agggcgtgcg cttcagtctg
     1081 cgcctgacca gtggcagtgg tgaaaacagc cgcctggtga ccagcgccat cactgccgac
     1141 acggagcacc gtttcagtgg cctgccgctc ggggaataca ccctgacggt cagggcgata
     1201 aacagctacg gccagcaggg cgaacctgcc accaccacat tccggattaa tgcaccggcg
     1261 gcaccggcca gcattgaact gacgccgggg tattttcaga taacagcagt accggtgctg
     1321 gcggtgtatg acccgacggt acagtatgaa ttctggttct cagaaaaacg catcacggac
     1381 acggcacagg tggaaacctc agcccgttat ctgggtaccg gcagccagtg gagcgtctcc
     1441 ggcccgcaca tcaggccggg gaaggatttc tggttttatg tgcgcagcgt caacctggtg
     1501 gggaaatctg cgtttgtgga agccagtggc cgggcgagca atgatgcgga agggtatctg
     1561 gactttttca gaggagaaat cgggaagaca catctggcac aggggatgtg ggagctgatt
     1621 gataacagcc agcttgatga tgagatggca gagatgaaga ccaccatcac agaaacccgc
     1681 aatgaaatca cacagacggt cagtaaaacc ctggaagacc agagtgccac catacagcag
     1741 atacagcggg tgcagacaga cacaaataac gacctggctg cgctgtacat gctgaaggtg
     1801 cagaaaacga aaaacggcat tccgtatgtt gccggtatag gtgcggggat tgaggatgct
     1861 gatggccagc ccctgagcaa tattctgctg caggcggacc gtatcgcgat gattaacccg
     1921 gagaacggca acaccacgcc gctgtttgtg gcgcagggga atcagctgtt tatgaacgac
     1981 gtgttcctga agcgactgtt tgcagtgagc atcacctcgt ccggcaatcc cccgacgttc
     2041 tccctgacgc cggagggcag gctgacggcc cgcaatgcgg acatcagcgg acatatcagt
     2101 gcgaactcgg gcacgctcaa taatgtcgtg atagcggaga actg
//
LOCUS       DAZFRN010000331         2097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFRN010000331 DAZFRN010000000
VERSION     DAZFRN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.334"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            257..711
                     /locus_tag="ACHKUB_005169"
                     /pseudo
     CDS             257..711
                     /locus_tag="ACHKUB_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
     gene            711..881
                     /locus_tag="ACHKUB_005170"
     CDS             711..881
                     /locus_tag="ACHKUB_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6658033.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            874..1164
                     /locus_tag="ACHKUB_005171"
     CDS             874..1164
                     /locus_tag="ACHKUB_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6658034.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1161..1523
                     /gene="rusA"
                     /locus_tag="ACHKUB_005172"
     CDS             1161..1523
                     /gene="rusA"
                     /locus_tag="ACHKUB_005172"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO6658035.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1520..1660
                     /locus_tag="ACHKUB_005173"
     CDS             1520..1660
                     /locus_tag="ACHKUB_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO6658036.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1746..>2097
                     /gene="quuD"
                     /locus_tag="ACHKUB_005174"
     CDS             1746..>2097
                     /gene="quuD"
                     /locus_tag="ACHKUB_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO6658037.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRL"
BASE COUNT          543 a          488 c          556 g          510 t
ORIGIN      
        1 tccatagttc tggctgtgat taggtgaaag tatacaaggt ttttaaagcg aagatcactg
       61 ttttggcttc aagatagttt tgatttatcg taatcaatcc gccataatcc tctcatcgga
      121 gcctgaacaa ctccggtgac ttctgcgcat ttaaggggac ttaaatgcga ccacaatctg
      181 aactcatcac cttgtcacag atgcagaaat gcacctgcga ttttctgcat tctgcggttt
      241 ccgttaggga ggccgtatga ctctgccagt agacggcatc aaactccatc gcggtaactt
      301 cgcggccatc ggccagcaga ttcagccatt gctggacgcc gggcaatgct tacgcctgca
      361 ggttaagccg tggcgcgaga agcgcagcct gtcgcagaac gcgctcagcc acatgtggta
      421 cacggagatc agcgaatatc tcatcgcccg cggcaagacc ttcgctacgc ctgagtgggt
      481 caaagacgcg atgaagcaca cctatctcgg ctacgaaagc aaagaccgtg tagatgtcgt
      541 gtccggcgag gtcaccaccg tccaatccct ccgccatacg tcagagcgga aaccggcgag
      601 atgtacatct tcctgtgcaa agtcgaagcc tgggcgatga atatcggctg ccacctgaca
      661 attccgcaga gctgtgaata ccagcagctg cgcgataagc aggaggcgta atggctacac
      721 cgcttattcg tgtcatgaac gggcacatct acagagtatc aaatcgtcgt aagcgtaagc
      781 ctgagctgaa gccatccgaa ataccaacac tgctcggata taccgctagc ctggttgata
      841 aaaaatggtt gcgactggca gcaaggagga atcatggctg atttgagaaa agcagcgcgt
      901 ggtcgggaat gccaggtaag aatccctggc gtatgtaatg gcaatcctga aacgtcagta
      961 ctggcacata tccggctggc tggattgtgc ggtaccggta tcaaaccgcc agacctgatt
     1021 gccaccattg catgttctgc ctgtcacgac gaaatcgacc gccgcacata ttttgtcgat
     1081 gctgagtatg caaaagaatg cgcgctggaa ggtatggcga gaacgcaggt tatctggctg
     1141 aaagaggggg ttattaaggc gtgaatacct acagcatcac attaccctgg cctccgagca
     1201 ataaccgcta ttaccgccat aatcgcgggc gcacacacat cagcgcagaa gggcaggcat
     1261 accgcgataa cgtcacccga atcattaaaa acgcaatgct ggatatcggc ctggctatgc
     1321 cagtgaaaat ccgtattgag tgccacatgc cggatcgccg tcgccgtgac ctggataatc
     1381 tgcaaaaagc cgcttttgac gcactcacca aagcaggttt ctggctggat gatgctcagg
     1441 tcgttgatta ccgcgttgtg aagatgcctg ttaccaaagg tgggaggctg gaactgacca
     1501 tcaccgaaat ggggaatgaa tgatgtttga gttttatatg gcagaacttc ttcgccaccg
     1561 ctgggggcat ctgcgcttat atcgtttccc cggttctgtt ttgaccgatt accgaatact
     1621 gaagaattac gccaaaaccc tgacaggagc aggagtatga agtcagagat aacaatcaac
     1681 taatactgtt ttgttgattt ttgcttgtaa ttggcgttct ggtctgattt ttgtggagta
     1741 agttgatgcg tgatattcag atggttcttg agcgttgggg agcgtgggcg gctaataatc
     1801 atgaagatgt gacctggtcg tccattgccg ccggttttaa gggattaatt acttcaaaag
     1861 taaaatctcg cccgcaatgt tgtgacgatg acgcgatgat tatttgtggg tgcatggccc
     1921 gtctgaaaaa gaacaacagc gatttgcacg atttattagt agattattat gtagtcggta
     1981 tgacattcat gtcactggca ggtaagcatt gctgctctga tggttatatc gggaaaaggt
     2041 tacagaaggc tgagggcata attgaaggga tgttaatggc attagatatc cggttag
//
LOCUS       DAZFRN010000332         2094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFRN010000332 DAZFRN010000000
VERSION     DAZFRN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.335"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..268
                     /locus_tag="ACHKUB_005175"
     CDS             <1..268
                     /locus_tag="ACHKUB_005175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658038.1"
                     /translation="NTFPVAFFSVYPHAIVVMTEITKKNKNNKIRGVLSGQNSFLIFI
                     GVYRQHYYEENKIIRKTEISCKADNRQPCSIITAGSFRLITEDR"
     gene            complement(294..1040)
                     /locus_tag="ACHKUB_005176"
     CDS             complement(294..1040)
                     /locus_tag="ACHKUB_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO6658039.1"
                     /translation="MKKIIAVSALAVVGMFSGQALADRGKTGFYMTGKAGASVVTQTD
                     QRFCQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARIHHKAT
                     YTDTSWNKSGEVSDFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLDAGDAS
                     VSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1830..>2094
                     /gene="subA"
                     /locus_tag="ACHKUB_005177"
     CDS             1830..>2094
                     /gene="subA"
                     /locus_tag="ACHKUB_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO6658040.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYI"
BASE COUNT          616 a          483 c          432 g          563 t
ORIGIN      
        1 caacacattc ccggtggcat tcttctcagt gtatccgcat gcaattgtgg ttatgacaga
       61 aataacaaaa aagaataaaa acaataaaat ccgtggtgtg ttgtccggac aaaacagttt
      121 tctgatattc atcggagtat atcggcaaca ttattatgaa gagaataaaa taataagaaa
      181 aacagaaata tcctgtaaag cagataacag acaaccctgc agtataatta cggcagggag
      241 tttccgttta attacagaag acaggtaatt attatctttc aggagtagtt tcgtcagaag
      301 ttataagtca caccaagcat gatgtcatga cttttaacat caacttctga tttatattta
      361 tcgccccact catccttgta actcacactg gcatcacctg catcaagata gcgatagctg
      421 aggtccagag cgatatccgg ggttacgtca tagcggacac ctgccccaaa actccatgca
      481 aaattgttat catatcccgg gtaatgcaac gcagaaaaat cgctcacctc accagattta
      541 ttccaggagg tatcagtata tgttgcctta tgatgtatcc gtgcataacc cagtccaaca
      601 gacacccatg gagtgaatgc actgctgttt ctgaagtcat aatacgtatt caccatcaga
      661 gtattcacac tgagcctgtt ctgtgtgtct tcccgaccac caccgaccat cggagagtac
      721 catgtatcca gtgtataacg ggagtccgca gctccgcggc catagaattc cacttccgtg
      781 cgcacaggaa cattgtaatg ctgataaaaa tcatagccca ccgcaaggcc ggcaccgaat
      841 accgtatcgt tcttatcgcc gcccttatac ttataaacat cgtccccaaa gtcctgacaa
      901 aagcgctggt cagtctgagt cacaaccgaa gcaccagctt taccggtcat ataaaagcct
      961 gttttgcctc tatcagccag ggcctggcct gaaaacatgc ccaccacagc aagcgctgaa
     1021 accgcaataa tctttttcat ttcagttatc tccgctatca ctttccccga acgataaacg
     1081 tggttgctta ttctaaagct atgcaaaaga cagggtaaaa agagaaaaat cgatcgaatc
     1141 gatcgacaaa atcgatcatg catgatgaag atttcttatc gaatacataa aaatagtgat
     1201 ggcaatcgtg gcaggagctt acgtcaggcc ctgaagtact gacacaggca ttgatggcag
     1261 gtgatataag gagtgctcca gggagtgact cccgactgac ccaggcactg tcccgttgtc
     1321 aacccgtatg gttactgctc tgactcattt cctgtcgtca gacgttcaac tctggaccac
     1381 tggttatgac gacaggtgtc gctcatttgt cagcactata acggtcaaag atcgtggcat
     1441 tgacacattc tccagaggta aatttctcaa gaggtaacaa ctctgccagg aggatatctg
     1501 cagagagtta cattcgtttg ggttcatatc tgtgccgcgc gttttccttc catcagtgaa
     1561 caaacattca gtcccatgac gaccgggcaa atagcgtgtt cacagatatt gctgtccagg
     1621 tacgaagtac cataatggat attggtaagt tattgggtga accccctcca tgaaaaaacc
     1681 aaatataaaa atgaatgttt cgggagagac ttcactcgct ttattgatag tgattgttta
     1741 tcttttactg aagacaggat atttcagatg tgatatccct gtaacatatt gaccagcaat
     1801 actttttatt tgtaccactg gaggatattg tgctgaaaaa tttacggctg catattctgt
     1861 tcctgctttt tatctcagtg tcagtcaggg cggaaaaacc atggtatttt gatgcaattg
     1921 gtctgacaga aacaaccatg tcgctcacag acaaaaatac cccggttgtc gtatctgttg
     1981 ttgattccgg agtggcattt gtaggaggcc tcagcgacag cgaatttgcc aaattcagtt
     2041 ttactcagga tggttcgcca ttcccggtaa aagagcctga agctttatat attc
//
LOCUS       DAZFRN010000333         2059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFRN010000333 DAZFRN010000000
VERSION     DAZFRN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.336"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            225..>2059
                     /locus_tag="ACHKUB_005178"
     CDS             225..>2059
                     /locus_tag="ACHKUB_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO6658041.1"
                     /translation="MNLRKKHICIAISLVLNPASISQVSAQADTNKLTVEVDETSLGH
                     DVYGDDWKSIEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTKEG
                     LYTGESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENN
                     YLASEAKGDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEAKGDMATAVGF
                     NALANAGNALAVGSQSSANKNDALAVGSEANAAQNETVALGHGATVTVAKGNVSIGAD
                     SRDKQAQKVTEAKVRTKRGAEVTYSGFAGNNAASVVSVGDAGKERQIVNVGAGAVSEK
                     STDAINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDG
                     DIAVTVNDKGMTIDGTTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVA
                     GDKLEISAETASLPVNGGNGKITVNNGDGDKLVTAKNIADAINQSGWKIKADKDTGGV
                     IEGNGQGTETLIKPGDMVTLKAGKNLSVKQSNGDFTFSVIDTPSFSSVQLNGNGKNGN
                     VTLSSEGNKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDK"
BASE COUNT          649 a          341 c          556 g          513 t
ORIGIN      
        1 tgtcgggtag tttacttaaa cttaatatgt tttatgttcg cattattata aatcacttta
       61 ttgccttttg tctgcttttt aatggttccc gttttgttca cttctggctt tataaaaaat
      121 ggatgctgaa gaaatgaagg cagtccacct tacataatat tcatgcatga tttttatgct
      181 attccgactg agtggaagaa aataaaggag aaaagagggt atatatgaac ctcagaaaaa
      241 aacatatctg tatagccata tcacttgttc ttaatccggc atccatatca caggtcagtg
      301 ctcaggcaga tacaaataag ttaactgttg aagttgatga aaccagcctt ggccatgatg
      361 tctatggcga tgactggaag agcattgaaa taggtacaac gaaatttaca gcaaaagatg
      421 aatcaaaggg aattaatttc gaccataaat caccggtggc tgaaggacgt aactctatcg
      481 cgattggtac cagtgcagag gctggaggtg acattagcac gaaggaaggt ttgtacacag
      541 gcgagtctat tgcgatagga gcgaaaacga aagcaactaa agaacagtca attgcaatag
      601 gtggcgatac caaagctacc ggatgggggg ctattgttat tggtggggat gatttaactc
      661 ctttgcatgg taagacttat ggtcgggaaa aaatagagaa taactatctg gcatcggaag
      721 cgaaggggga tggcagtatt gcgctgggca gcagaacttt tgctaaaggc gaattgtcag
      781 tatcgcttgg ttcggtgagt tctgctgagg ggatggcctc gaatgcaatt ggagccacgg
      841 cggaagctaa aggggatatg gcgactgcgg ttggttttaa tgcgctggct aatgccggta
      901 atgctctggc cgttggtagt cagtcctctg caaataaaaa cgatgctctt gctgttggtt
      961 cagaggcaaa tgccgctcag aatgaaacgg ttgccctggg gcatggagca accgttactg
     1021 tcgcaaaggg gaacgtgtcc atcggcgctg actccaggga taagcaggca caaaaagtaa
     1081 cagaggctaa ggtcagaact aaaagaggcg cggaagtcac atatagtggc tttgccggaa
     1141 ataacgcagc atcagtagta tctgttggtg atgcgggtaa agagcgtcag attgttaacg
     1201 tgggggcggg tgctgtatct gagaaaagta cagatgccat taacggtagt cagttatatg
     1261 cgatagcgaa gacactgaaa gatgaccttg atgccattaa tccgacgtgg acgctaaagg
     1321 atgataaggg aggttctgcg actattaatg gtgggaagga taataagaat ggtaacactt
     1381 taactgttaa aggtgatgga gatattgcag ttactgttaa tgataaagga atgacgattg
     1441 acggaacaac gctgaaaaat gaaataaaaa atgatgccat caaaattaaa ggtggagatg
     1501 gtaagacgct gacgaagagt cttggtgaca atgtcgaaat cactggcaaa ggcccaataa
     1561 aaacccaggt ggctggagat aaactggaga tttcagcaga gacagcttcg ttgcctgtaa
     1621 atggtggtaa cgggaagatt acagtaaaca atggtgatgg cgataaactt gttactgcga
     1681 aaaacattgc tgatgccata aaccagtctg gctggaaaat taaagcggat aaagataccg
     1741 gtggtgttat cgagggaaat ggtcagggaa cagaaacact gataaaaccc ggtgatatgg
     1801 ttacgctgaa agccggtaaa aatctcagcg tgaaacaaag taacggtgat tttacattct
     1861 ctgttattga tactccgtca ttcagcagtg ttcagctgaa cggtaatgga aaaaacggaa
     1921 atgtaactct ctcatccgaa ggtaataagc tgacattgtc aggcggtaat ggtgccggac
     1981 aaaatggtgt tgttatcagt ggtgtggcaa gtggactgga aggtaaggaa ctttcgacgt
     2041 taacgaatac tgataaaga
//
LOCUS       DAZFRN010000334         2056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFRN010000334 DAZFRN010000000
VERSION     DAZFRN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.337"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            8..559
                     /gene="ygiL"
                     /locus_tag="ACHKUB_005179"
     CDS             8..559
                     /gene="ygiL"
                     /locus_tag="ACHKUB_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO6658042.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVIITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            619..>2056
                     /locus_tag="ACHKUB_005180"
     CDS             619..>2056
                     /locus_tag="ACHKUB_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6658043.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYIN"
BASE COUNT          619 a          434 c          420 g          583 t
ORIGIN      
        1 gaatattatg tctgctttta aaaagtcact tcttgttgct ggcgtagcaa tgattctttc
       61 caataacgta tttgctgacg aaggtcacgg cattgttaaa tttaaaggtg aagttatctc
      121 tgctccatgt tcaattaaac caggtgacga agacttaact gtaaatctgg gtgaagtagc
      181 agataccgtt ctgaaaagcg accagaaatc tttggctgag cctttcacta ttcacctgca
      241 agactgcatg ttgagccagg gtggaaccac ttattcaaaa gcgaaggtta ctttcaccac
      301 agcgaatact atgacgggtc agactgacct gctgaaaaac accaaagaaa ctgaaatcgg
      361 tggtgccaca ggcgttggcg tacgtattct ggatagccag agcggcgaag tgactttagg
      421 tactccggta ataattacat tcaacaatac taacagctat caggaactga acttcaaagc
      481 tcgtatggaa tccccaagca aagatgctac cccgggcaac gtttacgctc aggctgacta
      541 taagattgct tacgagtaat cacattaggc cgggttcgcc cggccacatc attaacggat
      601 taatgataag tggaacagat gtataaaaaa ttaaaattaa ccacaataag cgaattgatt
      661 aaaaatattt attgttcatt atccgttctc atcattggtt gtgcgtcagc ttatgccgtt
      721 gaattcaaca aagatttaat cgaagccgaa gatcgtgaaa acgttaacct ttcccaattt
      781 gaaactgatg gccaattacc cgtaggtaaa tactcattaa acgctcttat taataataaa
      841 aggacgccaa tccatctgga cctgcaatgg gtattaattg ataaccaaac tgcagtttgt
      901 ctgactccag agcaattaac attattaggc tttactgatg agattattga agtggctcag
      961 caaaacctga tcgatggttg ttaccctatc gaaaaagaaa aacaaattac aacttatctc
     1021 gataaaggga aaatgcaatt atccatatct gcacctcagg catggttaaa atacaaagat
     1081 gcaaactgga cgcctcctga actttgggat catggtattg ctggggcatt tcttgactac
     1141 aatttatatg cctctcatta tgcaccacat cagggcgata attcacaaaa tataagttcc
     1201 tatgggcagg ctggggttaa tcttggggcc tggcgcctgc gtactgatta ccagtacgat
     1261 caatcattta acaatggcaa aagccaggcg aacaacctgg attttccgcg tatttatttg
     1321 tttcgcccaa tcccagcaat taatgcaaaa ctaactatag gtcaatacga tactgaatcc
     1381 tctattttcg actctttcca tttttctggc gtttcgttga aaagcgatga gaatatgttg
     1441 ccgcctgatc tacgtggtta cgcaccgcaa atcacgggtg ttgcacaaac gaatgcaaag
     1501 gtcactgtct cacagaacaa ccgtattatt tatcaagaaa atgttcctcc tggcccattt
     1561 gctattacta atttattcaa tacattacag gggcaactgg acgtcaaggt tgaagaagag
     1621 gacgggcaag tcacgcaatg gcaagttgca tctaatagta ttccttatct gacgcgtaaa
     1681 gggcagattc gctacaccac tgctatgggt aaaccgacca gcgttggtgg tgattcctta
     1741 caacaaccct tcttctggac tggtgaattc tcatggggtt ggctgaacaa tgtatccctg
     1801 tatggtggtt cagttttaac aaaccgtgat tatcaatctc tggctgccgg cgttggcttt
     1861 aaccttaact cattgggttc attatctttt gatgtcacac gatctgatgc tcagttgcat
     1921 aatcaggata aagaaacggg ttatagctac cgcgctaact attcaaaacg ttttgaatct
     1981 accggtagcc agctcacttt cgctggttac cgtttctctg ataaaaactt tgtgacaatg
     2041 aatgaatata tcaatg
//
LOCUS       DAZFRN010000335         2013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFRN010000335 DAZFRN010000000
VERSION     DAZFRN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.338"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>2013
                     /gene="rhsB"
                     /locus_tag="ACHKUB_005181"
     CDS             <1..>2013
                     /gene="rhsB"
                     /locus_tag="ACHKUB_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO6658044.1"
                     /translation="NPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEH
                     ADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQ
                     LTSATGPDGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTM
                     TWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQG
                     HETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLT
                     SENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRL
                     THRTVNGETAERWRYDERGWLTDISHISEGHRVAVHYRYDEKGRLTGERQTVHHPQTE
                     ALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVDFTRDRLH
                     RETLRSFGRYELTTAYTPAGQLQRQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSY
                     STTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSALSMWPDNRIARDAHYLY
                     RYDRHGRLTEKTDLIPEGGIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRYLYD
                     PLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRS"
BASE COUNT          484 a          621 c          607 g          301 t
ORIGIN      
        1 aacccggcag gcttaagcta cacgtatcag tatgagaaag accacatcac catcaccgac
       61 agcctggacc gccgtgaagt gctgcacacg cagggcgaag ccgggctgaa gcgggtggtg
      121 aaaaaggaac acgcggacgg cagcgtcacg cagagtcagt ttgatgcggt gggcaggctt
      181 aaggcacaga cggatgccgc aggccggaca acagagtaca gcccggatgt ggtgacgggc
      241 ctcatcacgc gcatcaccac gccggatggc agggcatcgg cgttttacta taaccaccac
      301 agccagttaa cgtcagccac cgggccagac gggctggaaa tgcgccgcaa atatgatgag
      361 tacggacgtc tgattcagga aaccgcccct gacggcgata tcacccgcta ccgttatgat
      421 aatccacaca gtgacctccc ctgcgcaacg gacgatgcca ccggcagccg gaaaaccatg
      481 acgtggagcc gttacggtca gttgctgagc ttcactgact gttccggtta tgtaacccgc
      541 tatgaccatg accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg gctgagtcag
      601 taccgcgcat acgacagccg tggacagtta attgccgtga aagacacgca gggccatgaa
      661 acgcggtatg aatacaacgc cgccggtgac ctgaccaccg tcattgcccc ggacggcagc
      721 agaaacggga cacagtacga tgcgtgggga aaagccatct gtaccacgca gggcggtctg
      781 acgcgcagta tggaatacga tgctgccgga cgggtcatcc gcctgaccag tgaaaacggc
      841 agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac cggctttgac
      901 ggccgcacac agcgttatca ccacgacctg accggcaaac ttatccgcag cgaggatgag
      961 gggctggtca cccactggca ctatgacgaa gcagaccgcc tcacgcaccg caccgtgaat
     1021 ggcgaaaccg cagagcggtg gcggtatgac gaacgtggct ggctgacaga catcagccat
     1081 atcagcgaag ggcaccgggt agcggtgcat tacaggtatg atgagaaagg ccgcctgacc
     1141 ggtgagcgcc agacggtgca tcacccgcag acggaagcac tgctctggca gcatgagacc
     1201 agacatgcgt acaacgcgca gggactggcg aaccgctgta taccggacag cctgcccgcc
     1261 gtggaatggc tgacctacgg cagcggttac ctggcaggca tgaagctcgg cgacacaccg
     1321 ctggtggatt tcacccgcga ccgcctgcac cgggaaacgc tgcgcagctt cggtcgttat
     1381 gaactcacca ccgcttacac ccctgccggg cagttacagc gtcagcacct gaacagcctg
     1441 cagtatgacc gcgattacac ctggaacgac aacggcgaac tcatccgcat cagcagcccg
     1501 cgccagaccc ggagttacag ctacagcacc accggcaggc tgaccggcgt tcacaccacc
     1561 gcagcgaatc tggatatccg catcccgtat gccacggacc cggcgggcaa ccgcctgccc
     1621 gacccggagc tgcacccgga cagcgccctc agcatgtggc cggataaccg tatcgcccgt
     1681 gacgcgcact atctttaccg gtatgaccgt cacggcaggc tgacagagaa aaccgacctc
     1741 atcccggaag ggggtatccg cacggatgat gagcggactc accggtacca ttacgacagt
     1801 cagcaccggc tggtgcacta cacgcggaca caatatgaag agccgctggt cgaaagtcgc
     1861 tatctttacg acccgctggg ccgcagggtg gcaaaacggg tgtggcggcg tgaacgggac
     1921 ctgacgggct ggatgtcgct gtcacggaaa ccgcaagtga cctggtacgg ctgggacggc
     1981 gaccggctga ccacgataca gaacgacagg agc
//
LOCUS       DAZFRN010000336         2000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFRN010000336 DAZFRN010000000
VERSION     DAZFRN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.339"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>2000)
                     /locus_tag="ACHKUB_005182"
     CDS             complement(<1..>2000)
                     /locus_tag="ACHKUB_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014715870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO6658045.1"
                     /translation="VNAAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGN
                     KIIADVGHDLLISSQQDTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDS
                     VAEQTGMFAGDGGFDITVGRHTQLEGAVIASTATPDKNHLDTGTLGFSDLHNEADYKV
                     SHSGISLSGGGSFGDKFQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQ
                     NLANLSRDPVHANDSISPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAA
                     QDKYGPVPADATEEQRQAYLAKLRDTPEYKKEQEKYGTGSDMQRGIQAATAALQGLAG
                     GNLAGALAGASAPELAHLLKSTEKDPAVNAIAHAILGGAVAAMQGNNVAAGAAGAATG
                     ELAARAIAGMLYPGVKQSDLSEEQKQTISTLATVSAGLTGGLTGNSTASAAVGAQSGK
                     NAVENNALSLPSGMVSYGQAVSSWNQYADANNLTPEQKQAGLDKIAKGELPEGANISK
                     VIVDGYKDGVLIAGAWYLGPAASVGKVIGGGVIAEIANGTYQWFDLSQPGNENKNWDW
                     KSSASAGITGMLAPGRTVGQNVGIAMGSAFFTDGPNAGAIGGAAAGAWAGGLFGEYAP
                     GIVNSVTGKEIPGFVYDYWGGVASEFSSGFIKDLNKPKGSSE"
BASE COUNT          401 a          622 c          435 g          542 t
ORIGIN      
        1 ctctgacgaa cctttcggtt tattgagatc tttaataaat ccagatgaaa actctgaagc
       61 aacccctccc caataatcat aaacaaaccc aggaatttcc tttccagtaa cagagtttac
      121 aattccaggt gcatattccc caaataatcc accggcccat gcacctgcgg cagcacctcc
      181 aatagctcct gcattcggtc catcagtaaa aaatgcactg cccatcgcaa ttcctacgtt
      241 ttgtccaaca gttcttcccg gtgcaagcat accagtaatg cctgcactgg cactgctttt
      301 ccagtcccag tttttgttct cattacccgg ctgactcaga tcaaaccact ggtatgttcc
      361 gttagctatc tcagcaataa ctccaccacc gatcactttc ccgacggacg ctgcaggccc
      421 cagataccag gcacctgcaa tcaatacgcc atctttataa ccatcaacta taactttgct
      481 tatattggct ccttcaggta actcaccttt ggcaatttta tccagtcctg cctgcttctg
      541 ttccggggtg agattattgg catcagcata ctgattccat gaggaaaccg cctgaccata
      601 actcaccatt cctgatggca gactgagcgc attattctca acagcattct tcccgctttg
      661 tgctcccact gctgctgatg cggtgctgtt tccggttaga ccgccggtca gccctgctga
      721 aacggttgcc agcgtactta tcgtctgctt ctgctcttca ctgaggtcag actgcttcac
      781 gcccggatac agcattccgg caatcgctct ggctgccagc tctcccgttg ctgcgcctgc
      841 tgcaccggca gcaacattat taccctgcat tgccgctact gcaccgccca gaatggcgtg
      901 agcaatggcg tttactgccg ggtctttttc tgttgatttc agcagatgcg ccagttccgg
      961 tgcagaagct ccggccagag cacccgctaa attaccaccc gccaggccct gaagtgcagc
     1021 cgttgcagcc tggataccgc gctgcatatc gctgccggta ccatactttt cctgttcctt
     1081 tttgtattcc ggcgtatcac gcagttttgc cagatatgcc tgccgctgtt cttccgtcgc
     1141 atccgccgga acaggcccat atttatcctg cgcagccttc aatgcattca gttccccctg
     1201 tgtacgcgca atatcagcca cctgactgcc tatgtcactg ataagcccca ctgtctgcag
     1261 acgcctctgc tccttctcct tgtcaaatat cgggctgata ctgtcattag cgtgcacagg
     1321 gtcacggctc aggttcgcca gattctgctt ctgattgtcc ctgtcccgga tggtgatagt
     1381 gccttctgcc actgcggcct gagtcgttcc ttccgcatgt ccgctgtgac ctccggctga
     1441 tatcatacca cccggcatgt taccctgaaa tttatccccg aagctgccac caccgctcag
     1501 actgattcca ctgtgactga ctttataatc cgcttcgttg tgaaggtcac tgaaccccag
     1561 cgttccggta tccaggtggt ttttatccgg tgtggcagtg gaggcaatca ccgcaccctc
     1621 cagttgggta tgtctgccca ctgtaatgtc gaagccgccg tcaccggcaa acatcccggt
     1681 ttgttcagca acggagtcaa agcggctctt cattttgtcc cgggaggcag cgatgtaacc
     1741 tgagccggtc atggagccaa aggtaaaact gccgccggca gccacgctgg tctgtttact
     1801 gtcgtactta ctggtgtcct gctggctgct tatcagcagg tcgtggccca catcggcgat
     1861 aattttattg ccgttgacct gagcaccgtt cagtaccgta tcccgaccac tgttgatggt
     1921 gacggtttta ccgctgtctg ttgtggtttc agtccactca gtaccgttac ctttctcgct
     1981 gccttttgcc gcattaacgc
//
LOCUS       DAZFRN010000337         1998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFRN010000337 DAZFRN010000000
VERSION     DAZFRN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.340"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1998
                     /locus_tag="ACHKUB_005183"
     CDS             <1..>1998
                     /locus_tag="ACHKUB_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658046.1"
                     /translation="LPTVSISVETTSTDVNGDGFTGIASVNGTVMDVPATIEDKDDST
                     GLVYTVSLNHVTTTDVTVTVTLGSGAGHSDAADYSDIGGAQHNGKIGLHGDTGKVTYD
                     GATTVTIVIPAGSKSVSFIVDPTLEANQDAFNAEGMEKVVATITGTSDNVTAATDIVD
                     NAGASATGVIYDGNAISLRNLDGDFTLKYSLSSSIAEKGDFGYTIGANSGENDPMVTT
                     DYNDTVYVGYYQSGKETTSYSNVANSQDNGPDGTKTDGNQSITTVDLGAGDDLMVIRG
                     NMLANTRVYTGEGNDTFTMDGMNTALRVMYAGSYIFTESGDDIVTIKRTGVTNAGQIY
                     LGSGSDTFIQGDATDNNDTTLSGLLDLGSGTKDISNMPKEYLSVYQDGSNLSLGNDNN
                     IDTATDVNTVTIYGSVSGEILGGYGSDNITVTKNLTGNISVGDNADTLTAGWIYGGST
                     VSMGDGNDTVTITDGAYNTTISLGAGDDVFDATSGVMGDSAYATVVNGEDGNDTFKLG
                     TIAKNLTIDAGAGDDIVVLTKDYDSTSSGNQGYINGGEGSDTLVLTGTISVNLAAGKN
                     EGIAGFEKVDMTVGSDLKADNAAQLVKLTASDVLGINDNSTIYISGDANDKVDLGADG
                     AGSLGTFTATATTVKATALDGIEHTYTLYSSVSGANVYIDN"
BASE COUNT          580 a          341 c          497 g          580 t
ORIGIN      
        1 gttaccaaca gtgagtatta gcgttgagac aacatcaaca gatgtcaatg gtgatggttt
       61 tactggtata gctagtgtca atgggaccgt aatggatgtt cccgcaacta ttgaagataa
      121 ggatgattcc actggactgg tttatacggt ttctctgaat catgtcacta caacagatgt
      181 aacagttaca gttacattgg gtagcggagc tgggcattct gatgctgctg actattctga
      241 tattggtgga gcacagcata acgggaaaat tggccttcat ggtgatactg gaaaagtaac
      301 ctatgatggt gcaacaaccg taacaattgt tattcctgcc ggttcaaaaa gtgtttcatt
      361 tatagttgat ccaacgcttg aggctaacca agatgccttt aatgccgagg gtatggaaaa
      421 agttgttgcg accattacgg gaacatctga taacgttacg gcagctactg acatcgttga
      481 taatgcggga gcatccgcaa caggtgttat ttatgatggt aatgccattt cattacgtaa
      541 tctggacggt gattttacac tgaaatattc actatcatct tctatcgccg aaaaagggga
      601 ctttgggtat accattggcg cgaatagtgg tgaaaatgat ccaatggtaa caacagatta
      661 caatgatacg gtttatgttg gttattacca gagtggtaaa gaaacaacat cgtatagtaa
      721 tgttgctaac tcacaggata atgggccaga cggaacaaaa accgatggca atcaaagcat
      781 tacgactgtt gatttaggtg ctggtgatga tctaatggtc attcgtggta acatgctggc
      841 taatacaaga gtatatactg gtgagggtaa cgatactttt actatggatg gtatgaatac
      901 tgcattacgt gttatgtatg cgggtagtta tatctttact gagtctggcg atgatattgt
      961 tacgatcaaa cgtactggtg taacaaatgc cggacagatt tatcttggtt caggtagtga
     1021 tacatttatc cagggggatg ctactgacaa taacgataca acattatctg gtttattgga
     1081 tcttggtagt ggtacaaaag atatatctaa tatgcctaag gaatatcttt ctgtttatca
     1141 ggatggcagt aatttaagct tgggtaacga caacaatatt gacactgcca cagatgttaa
     1201 taccgtcact atttatggct ctgtgtctgg tgaaatttta ggcggatacg gcagtgataa
     1261 tattacggta actaaaaatc tgaccggtaa tatttctgtc ggtgacaatg cggatacctt
     1321 aacggccgga tggatttatg gtggcagtac tgtctctatg ggggacggca atgatactgt
     1381 taccatcaca gatggtgcat ataacacgac tatttcactg ggggcgggag atgatgtatt
     1441 tgatgcaact tctggtgtaa tgggggattc tgcttatgcc actgtcgtta atggtgaaga
     1501 cggcaatgat acctttaaat taggcacaat tgccaaaaac ctgaccattg atgccggtgc
     1561 cggtgatgat attgttgttc tgacaaaaga ctatgactcc acctcctccg gtaatcaggg
     1621 ctatattaac ggcggggaag gcagtgatac cctggttctg acaggtacca tttctgtcaa
     1681 tctagcggcc ggtaaaaatg aaggtattgc cggcttcgag aaagtggata tgactgttgg
     1741 tagcgatctg aaagctgaca atgcagctca gttagtcaag ttaacggcat cggatgtatt
     1801 gggtataaat gacaattcaa cgatttatat ttccggtgat gccaatgaca aagttgattt
     1861 gggggctgac ggagcgggaa gtctgggtac atttacggca acggcaacaa ctgttaaagc
     1921 aactgcgtta gatggtattg agcatactta tacgttatac agcagtgtaa gtggcgcaaa
     1981 tgtgtatatt gataacaa
//
LOCUS       DAZFRN010000338         1968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFRN010000338 DAZFRN010000000
VERSION     DAZFRN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.341"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            673..1449
                     /locus_tag="ACHKUB_005184"
     CDS             673..1449
                     /locus_tag="ACHKUB_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6658047.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGIFGYIMHRTMPGISFPVFLLNGLIPFFIFNSISNRSVEAIDANQGLFNYRPVKP
                     MDTIIARTLLETLIYIIVYVLLMLIVWMSGEYFEIISLLQLVLVWTLLIIISCSVGLI
                     FMVVGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQYWTYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            1446..>1968
                     /locus_tag="ACHKUB_005185"
     CDS             1446..>1968
                     /locus_tag="ACHKUB_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6658048.1"
                     /translation="MIKIENLTKSYRTSTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDRGKIITNKTISWPIGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     DKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKERHKES"
BASE COUNT          574 a          351 c          437 g          606 t
ORIGIN      
        1 agctattaga tgtaaattac tttggtcagg tccttcacag cagaaacaga atgtcatgaa
       61 agataaattt acgcagtcac ttgatcaact gcttagtttt gcgtcttaaa ggattgtaac
      121 tatggcaact gtggagcgaa aaatcattgg gcacgggaaa agtgcggtca tatagaaaag
      181 tgctctctat tcagcatctc atggtttatc agcgaagaca gaaaaaatta taacgatgcg
      241 caggaatttg tggaggtttg ctaatataga gcgattcgag catccctcgt taaatctctc
      301 gaaccgcagt atattcagac atttttaaaa tgcgccagat gattacgaca gaaagaacac
      361 aacatataat tcatgttcgc tgttctcttg ctgaatacgg aattgtcttc aataaaggtg
      421 ctacacatta tggtgagagc taccacggtt tcttgaggat gcagaaaatg gatttaccga
      481 tatcgtgcaa ttttgtatcg gtagtatgtc cgactcatgt ctcatggata atgaaccata
      541 tattataact gcgggtagaa aaatgcgcat ctaacgactt tgcctggacg tgtcgattaa
      601 ctgttcctgg gtttgctact cttgtcagtc agacgataaa agtgagcata aatatatagg
      661 attacatatc aagtgaaaag aagtggattt gaagttcaga aggtgacagt agaggcatta
      721 tttctacgag aaatacgaac acgttttggt aagtttcgtc tgggatatct gtgggcaatc
      781 ctggaacccg ctgctcattt gctggtgctg ttaggtattt ttggctatat tatgcatcgt
      841 acgatgccag gtatctcgtt cccggtattt ttacttaatg gtttgattcc cttttttatc
      901 tttaatagta tcagtaatcg gtctgtggaa gctattgatg caaaccaggg gctttttaat
      961 tatcggccag taaagcccat ggatacaata atagccagaa ccctgcttga gacactaatt
     1021 tacattatag tttatgtgtt gcttatgctt attgtctgga tgtcaggaga atattttgaa
     1081 ataataagtt tattacaact ggtacttgta tggactcttc tgattattat ttcatgtagt
     1141 gtcggattaa tatttatggt cgttggtaaa actttccctg aaatgcaaaa gattctaccg
     1201 atatttctta aaccgctgta tttcatttcc tgtatcatgt tccctctaca ttcgatccca
     1261 cagcaatact ggacatatct actctggaac ccattagtcc atgtcgtaga gttaagtcgt
     1321 gaggcagtta tgccgggcta tatcagcgaa ggcgtgagtt tgaactatct ggcaatgttc
     1381 actctggtca ccctgttcat cggtctggcg ctataccgaa cgcgtgaaga ggcaatgctg
     1441 acatcatgat taagattgag aatctgacga agtcatatcg cacgtcaaca ggtcgacact
     1501 atgtgtttaa ggatttaaat atcgaactcc cttcaggaaa aagtgttgcc tttattggtc
     1561 gtaatggtgc aggtaaatca actttactga gaatgattgg cggtattgac cgccccgata
     1621 gaggaaagat catcaccaat aaaacgatat catggcccat tgggctcgcc ggtggattcc
     1681 agggcagttt aaccgggcga gaaaacgtaa aatttgttgc acggttatat gcgaagccag
     1741 aagaactgaa agataaaatt gagtttgtcg aagaatttgc cgaactcggt aagtactttg
     1801 atatgccgat caaaacttac tcatctggta tgcgatctcg cctgggtttt ggtttaagca
     1861 tggcatttaa gtttgattat tatattgtcg atgaagtaac ggcagtcggt gatgccaggt
     1921 ttaaagaaaa atgtgcacat tactttaaag aaaggcataa agagtcta
//
LOCUS       DAZFRN010000339         1948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFRN010000339 DAZFRN010000000
VERSION     DAZFRN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.342"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..810
                     /locus_tag="ACHKUB_005186"
     CDS             <1..810
                     /locus_tag="ACHKUB_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO6658049.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            877..>1948
                     /gene="rhsD"
                     /locus_tag="ACHKUB_005187"
     CDS             877..>1948
                     /gene="rhsD"
                     /locus_tag="ACHKUB_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO6658050.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGLWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAG"
BASE COUNT          435 a          531 c          621 g          361 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg attattatgg ggcgtaccta ccaccaggaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgaccattcg ttcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa
      841 caagcattaa aaataaacag aagaaggaat tgaatcatga gcggaaaacc agcggcgcgt
      901 cagggagata tgactcagta tggcggtcct attgtccagg gttcggcagg tgtaagaatt
      961 ggcgcgccca ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg
     1021 gtaaatccgc tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc
     1081 ccgctgccgt tcattctctc ccgcacctac agcagctacc ggaccaggac acccgcgccg
     1141 gtgggagtgt ttggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgcgat
     1201 gatgcactgg tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg
     1261 ggggaggcgg tgtacagccg cagcgagtca atgtggctgg tgcgcggtgg taaggcagcg
     1321 cagccggacg gccacacgct ggcgcggctg tggggggcgc tgccgccgga tatccggtta
     1381 agcccgcatc tttacctggc gaccaacagc gcacaggggc tgtggtggat actggggtgg
     1441 tcagagcggg tgccgggtgc tgaggacgta ctgccagcgc cgctgccgcc gtaccgggtg
     1501 cttaccgggc tggcagaccg tttcgggcgg acgctgacgt accggcgtga ggccgcaggc
     1561 gacctggccg gggaaatcac cggcgtgacg gacggtgccg ggcgtgagtt ccgtctggta
     1621 ctgaccacac aggcacagcg ggcggaagag gcccgcacct cttcgctatc ttcttctgac
     1681 agttcccgcc ctctctcagc ctcaccgttc cccgacacac tgcccggtac cgaatacggc
     1741 cccgacaggg gcatccgtct ttcggcggtg tggctgatgc acgacccggc atacccggaa
     1801 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
     1861 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
     1921 cggatggtgg cgcaccgtta cgcgggaa
//
LOCUS       DAZFRN010000340         1868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFRN010000340 DAZFRN010000000
VERSION     DAZFRN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.343"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            50..343
                     /gene="bor"
                     /locus_tag="ACHKUB_005188"
     CDS             50..343
                     /gene="bor"
                     /locus_tag="ACHKUB_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO6658051.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(434..616)
                     /gene="rzoD"
                     /locus_tag="ACHKUB_005189"
     CDS             complement(434..616)
                     /gene="rzoD"
                     /locus_tag="ACHKUB_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO6658052.1"
                     /translation="MLKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(833..1330)
                     /gene="rrrD"
                     /locus_tag="ACHKUB_005190"
     CDS             complement(833..1330)
                     /gene="rrrD"
                     /locus_tag="ACHKUB_005190"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO6658053.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(1330..1545)
                     /gene="essD"
                     /locus_tag="ACHKUB_005191"
     CDS             complement(1330..1545)
                     /gene="essD"
                     /locus_tag="ACHKUB_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO6658054.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          511 a          462 c          403 g          492 t
ORIGIN      
        1 ttctacacat acgattctgc gaacttcaaa aagcatcggg aataacacca tgaaaaaaat
       61 gctactcgct actgcgctgg ccctgcttat tacaggatgt gctcaacaga cgtttactgt
      121 tcaaaacaaa ccggcagcag tagcaccaaa ggaaaccatc acccatcatt tcttcgtttc
      181 tggaattggg cagaagaaaa ctgtcgatgc agccaaaatt tgtggcggcg cagaaaatgt
      241 tgttaaaaca gaaacccagc aaacattcgt aaatggattg ctcggtttta ttactttagg
      301 catttatact ccgctggaag cgcgtgtgta ttgctcacaa taattgcatg agttgcccat
      361 cgatatgggc aactctatct gcactgctca ttaatatact tctgggttcc ttccagttgt
      421 ttttgcatag tgatcagcct ctctctgagg gtgaaataat cccgttcagc ggtgtctgcc
      481 agtcgggggg aggctgcatt atccacgccg gaggcggtgg tggcttcacg cactgactga
      541 cagactgctt tgatgtgcaa ccgacgacga ccagcggcaa catcatcacg cagagcatca
      601 ttttcagctt tagcatcagc taactccttc gtgtattttg catcgagcgc agcaacatca
      661 cgctgacgca tctgcatgtc agtaattgcc gcgttcgcca gcttcagttc tctggcattt
      721 ttgtcgcgct gggctttgta ggtaatggcg ttatcacggt aatgattaac agcccatgac
      781 aggcagacga tgatgcagat aaccagagcg gagataatcg cggttactct gttcattgct
      841 gaccccacaa acagatttca cgctcaatct cacgacgagt catgagacct ttccattgct
      901 taccgccagc atatgtccag cgacgtagct gatcacatgc gcctttgata tcgccctggt
      961 ttattttgcg aagaagcgtc gatgttctga aattgccagc acccacgttg taaacgaatg
     1021 agtaaagagc gccgcgcgtt gtttccggta tatcgacttt gatgtacggg ttaatttgtc
     1081 tggcgacagt ggcaaggtct ttattcaaga gtgctttgca ttctgctttg gtatacgttt
     1141 taccgagcat gatgtctttt cctgtatgcc cgtgacatac agtccataca ccaacaatat
     1201 ctttatatgg tatgtagctg acaccttcca gaccatcgtc accactcgga ccagtgatga
     1261 gcacagacgc tatggcaaca gccccaccac caatagcagc tgctacagcc ttgcgtaatg
     1321 atggcgacat tattcacctc tcgcagcctt acgcttatct tctttaatct tgaaataaag
     1381 gtttgtcagg tacgtcagca ggccaaatac caggctaccc agcactccaa ttgccgccca
     1441 ctgtgagggc gtgactttat ctagcagctg taaaaaccag tacccggcac tacctgctga
     1501 ggtgccatag gcgacacccg ttgttaactt atccatggat ttcataaccc cacctcgcag
     1561 acaaagcggg tgtaaattga gggaatacta cgaaacgtaa cagactcgga gtcagtgaat
     1621 aactcaggta ttgggttatc agctaatatc gagactcaaa aaatggaaaa acccgctcga
     1681 cggcgggttt aagctgtgtg acgaagtaac cactcttaac agcataacca attttttacg
     1741 tacgtaaacc actaaatgat atttgcgaga atgctaccga gtattgaaaa caccactaca
     1801 aatacataag caaatctcaa caaacaacca acaaataatt tccagtgtta tttttagccg
     1861 atttaaat
//
LOCUS       DAZFRN010000341         1841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFRN010000341 DAZFRN010000000
VERSION     DAZFRN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.344"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..292
                     /locus_tag="ACHKUB_005192"
     CDS             <1..292
                     /locus_tag="ACHKUB_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658055.1"
                     /translation="NNIIDSRNISAPHELANILTREKILTTREKKGNLISLFDGFTLF
                     HCVGMITFLIHYLRTPEEKVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
     gene            complement(480..1682)
                     /locus_tag="ACHKUB_005193"
     CDS             complement(480..1682)
                     /locus_tag="ACHKUB_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6658056.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT          525 a          455 c          354 g          507 t
ORIGIN      
        1 aaataacatt atagacagca gaaacatatc agccccccat gaactggcaa atattcttac
       61 ccgcgaaaaa atacttacaa cacgggaaaa aaaaggaaac ctgataagcc tgtttgatgg
      121 attcacttta ttccattgtg ttggaatgat aacctttctt atccattatc tcagaacacc
      181 tgaagaaaaa gttgaaaata tatttatgtt atatggtgca gataaaaaca ataaactacg
      241 cagaagactg atttatgacg cactaggaat aattcagtct cagcaggagt gaaacgttaa
      301 aacaggcaaa ttgtttttta taaacaggag attgttgagt aatcccctgt ttataaaaat
      361 acgctttcct gacgatctga taattatcag ctcaatacat ttgacataaa agcggttcct
      421 taattactac tgccacgatc acatatcaaa tcatgatcgt acaatataca ttttcactat
      481 tatttattca caatttgaga catcacataa tccgcccatg cctgcatcat cggtacacgc
      541 tgctccagta gatcagtacg atgatatgcc gcctcaacct tatttttcag cgtatgagcg
      601 agcgcccttt ccgccagatc ccgcgaatac ccctgttcac tacaccagtc cctgaatgtt
      661 gagcgaaaac catgtgccgt ggcaactcgc cccggaatgt cactgacggc tttcttttta
      721 cgcagaaaac ttgtcaacac catatcggaa aggatctgct gctttctggg tgaagggaac
      781 accagttcat catgcaggcc acgtatattt tccagaatgt aaatagcctg ccgggataaa
      841 ggaacacgat gctgtagcct ggctttcatt ctttctgcag gtatagtcca tacccgctta
      901 tgaaaatcaa tttcagccca gcgcattccc ctggcttcgc ccgagcgagt tgctgtaagt
      961 atcaccatta ataacagtgc gcgggtaaca ttataaggtt catcactata cacactggtc
     1021 gccacaaaaa gcggtaactg cctccagggc attgctggtt ggtgttcatc acgtcctctt
     1081 gtctgctgag gaagcaaatg gtcaaccaca tcaacaggat ttgccacaca aaaaccgtgc
     1141 gcccatcccc actgcataac aacatgaatg cgctgtttaa cccggcttgc cgtttctgac
     1201 aaggttaacc agactggacg cagtgtttct gccacgtccg cagccgtaat cgaatccagc
     1261 gtttttgctc ccagttgagg aaacgcgtaa ttctcaagcg tcgataacca ctgccttaca
     1321 tgctttggat tttcccatcc aggagacagt tctgcatgta cacgcctggc tgcatcggca
     1381 aatgttggga taacgacttt ctcagattca gcctttttaa tctccagggg atcatcacct
     1441 gcagcaagtt gctctcgcat tatccgggca gtacgtgcag cttcagcaat actgacctct
     1501 gggtaagttc ccaatcctgc attacgtctt ttttgtgtca ccggacttac ataacgaaaa
     1561 acccatttcc cccgcccctt tactgaagaa ggatgaaggg tcagtccggt aattccccca
     1621 tggggcaatg gtttgtcatc aggtttgata tgtcttgctt tcgtatccgt caatactgcc
     1681 atacgctaat tcccgtcact ctggtatgcc atccagtatg ccattagtct ctcgcttcac
     1741 taagaataca ttagataata tcggacaaca aaaagtgtaa tgcattgttt ataatgaaat
     1801 tatataacac ccctggatta agtcagattt atttcaggcg g
//
LOCUS       DAZFRN010000342         1837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFRN010000342 DAZFRN010000000
VERSION     DAZFRN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.345"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1837
                     /gene="rhsB"
                     /locus_tag="ACHKUB_005194"
     CDS             <1..>1837
                     /gene="rhsB"
                     /locus_tag="ACHKUB_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO6658057.1"
                     /translation="NPAGLNYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEH
                     ADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQ
                     LTSATGPDGLEIRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTM
                     TWSRYGQLLSFTDCSGYQTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQG
                     HETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLT
                     SENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWYYDEADRL
                     THRTVNGETAERWQYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTE
                     ALLWQHETRHAYNAQGLANRCIPDSLPAVEWLAYGSGYLAGMKLGDTPLVDFTRDRLH
                     RETLRSFGRYELTTAYTPAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSY
                     STTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLY
                     RYDRYGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQY"
BASE COUNT          444 a          579 c          543 g          271 t
ORIGIN      
        1 aacccggcag gcttaaacta cacgtatcag tatgagaagg accgcatcac catcaccgac
       61 agcctggacc gccgtgaagt gctgcacacg cagggcgaag ccgggctgaa gcgggtggtg
      121 aaaaaggaac acgcggacgg cagcgtcacg cagagtcagt ttgacgccgt gggcaggctc
      181 agggcacaga cggatgccgc aggcaggaca acagagtaca gcccggatgt ggtgacgggc
      241 ctcatcacgc gcatcaccac gccggatggc agggcatcgg cgttttacta taaccaccac
      301 agccagttaa cgtcagccac cgggcctgac gggctggaaa tacgccggga atatgatgaa
      361 tccggccgtc tgattcagga aaccgcccct gacggcgata tcacccgcta ccgttatgat
      421 aatccacaca gtgacctccc ctgcgcaacg gacgatgcca ccggcagccg gaaaaccatg
      481 acgtggagcc gttacggcca gttgctgagc ttcaccgact gttccggcta ccagacccgt
      541 tatgaccatg accgcttcgg gcagatgacg gcggtgcacc gcgaggaagg gctgagccag
      601 taccgtgcat acgacagccg tggacagtta attgccgtga aagacacgca gggccatgaa
      661 acgcggtatg aatataacgc cgccggtgac ctgaccaccg tcattgcccc ggacggcagc
      721 agaaacggga cacagtacga tgcgtgggga aaggccatct gtaccacgca gggcgggctg
      781 acgcgcagta tggaatacga tgctgccgga cgggtcatcc gcctgaccag tgaaaacggc
      841 agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac cggctttgac
      901 ggccgcacac agcgttatca ccacgacctg accggcaaac ttatccgcag cgaggatgag
      961 gggctggtca cccactggta ctatgacgaa gcagaccgcc tcacgcaccg caccgtgaat
     1021 ggcgaaaccg cagagcggtg gcagtatgac gaacgcggct ggctgacaga catcagccat
     1081 atcagcgaag ggcaccgggt gacggtgcat tacgggtatg atgagaaagg ccggctgacc
     1141 ggtgagcgtc agacggtgca tcacccgcag acggaagcac tgctctggca gcatgagacc
     1201 agacatgcgt acaacgcgca gggactggcg aaccgctgta taccggacag cctgcccgcc
     1261 gtggaatggc tggcctacgg cagcggttac ctggcaggca tgaagctcgg cgacacaccg
     1321 ctggtggatt tcacccgcga ccgcctgcac cgggaaacgc tgcgcagctt cggccgttat
     1381 gaactcacca ccgcttatac ccctgccggg cagttacaga gccagcacct gaacagcctg
     1441 cagtatgacc gcgattacac ctggaacgac aacggcgaac tcatccgcat cagcagcccg
     1501 cgccagaccc ggagttacag ctacagcacc accggcaggc tgaccggcgt tcacaccacc
     1561 gcagcgaacc ttgatatccg catcccgtat gccacagacc cggcaggtaa ccgcctgccc
     1621 gacccggagc tgcacccgga cagcaccctc agcatgtggc cggataaccg tatcgcccgt
     1681 gacgcgcact atctttaccg gtatgaccgt tacggcaggc tgacggagaa aaccgacctc
     1741 atcccggaag gggttatccg cacggatgat gagcggactc accggtacca ttacgacagt
     1801 cagcaccggc tggtgcacta cacgcggaca caatatg
//
LOCUS       DAZFRN010000343         1817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFRN010000343 DAZFRN010000000
VERSION     DAZFRN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.346"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            75..1322
                     /locus_tag="ACHKUB_005195"
     CDS             75..1322
                     /locus_tag="ACHKUB_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO6658058.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            1608..>1817
                     /locus_tag="ACHKUB_005196"
     CDS             1608..>1817
                     /locus_tag="ACHKUB_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658059.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEG"
BASE COUNT          535 a          339 c          521 g          422 t
ORIGIN      
        1 gcgataactt catgagcgtc aaccggataa gaaaaaagag cagatatgtt taacagtttg
       61 tggaccgcag ggatatgaca gaaaaaacgg aacggaataa agcgtcgtca gaggtggtca
      121 ggcagtcaga cctggcgctg ttgacagatg tacaggcagc ccttcagcag gaaaagcatc
      181 atgccctttt cctgatggtg atttttctgc tggtactggt agtgacgttt gttatctggg
      241 catggaacag tcctctggat gaagtgaccc gaggtcaggg gagtattatt cccggcagca
      301 gggagcaggt tatacagacc cttgacccgg gtatactgaa atcacttgag gtcagggaag
      361 gcgatattgt tgagaaaggt caggtactgc tgacactgga tgatacccgg agctctgcca
      421 tgctgaggga aagtgaagcc cgggtgaata acctggaggc cgtcagggca agactccgtg
      481 cagaagctta ttccgaatca ctgacatttc cggataacgt tcctgaggat ttgcgggagc
      541 gggaaagtac ggtctatttt ctccggaaga cggagctggc gcagagcatt gccggtctga
      601 agcagagtaa ggcgctgctg gacagggaaa tagcaatgac gcggccaatt gtgcgtgaag
      661 gggcaatgtc ggaggtcgaa ctgttacgta tgcagcgtca gtcagcggag ctgcagttac
      721 agatggatga gaaacagaat aaatacctga cggaagccgg tgctgaactg gtgaagacag
      781 aagcagagct tgctcaggca aaagagaata tggctggcag agccgacccg gttgagcgtt
      841 cacgtctcag ggcccccctg agaggtatcg taaaaaatat acgggtaaac acactgggag
      901 gggtggtttc tgcaggacag gacataatgg aaattattcc gctggaagac cagttgctga
      961 ttgaagctta tataaatcca cgggatgttg cctatgtcag aacagggatg ccggcacttg
     1021 taaaactgac ggcttatgac tatgccattt atggtggtct tgacggcgtg gtgacactgg
     1081 taagtccgga tactttacgt gaccagaaac ggccgggaga tttaaaactg gacccgaatg
     1141 aagcttatta ccgggtactg gttaccacca gtaataatta tctgacagac aggaacggaa
     1201 aaagattgcc agttattcct ggcatgatag ccagcgtgga tataaaaacc gggcgtaaaa
     1261 cggtatttca gtatctgatt aaaccgataa ccagaatgaa gcaggcactt caggaaagat
     1321 aataaaaagg tcttccgggg aaagtatttt aaatcaattt taaacgggca gaatgatttc
     1381 actttattct gcccgggtca ttgcgcatgc ctgtatgtga tttggggctg aagataatat
     1441 aaatgttata tctgtcaggg gaaaataaat ctgaaaaaag cggaatgatt aaggaggtcc
     1501 ggtcagagaa ataaattatc tgtaatctga ttcgttttta tttaatgact ccctttagcg
     1561 gataaaacat aagagcatac tggttttatt aacctgagga tacttatatg aaaatgattt
     1621 tcaccggcaa ggtcagtgga gaaaaaacag tactgacagc cggagcccgt catacagtaa
     1681 aggcacaggc cggggaacag tatggactgg ttgatgaggt gacaggtctg gtcccggatg
     1741 gtgtggaagc ggaccgttct ggtgatgatt taattctgcg taaaaaggaa gatgatacag
     1801 aaatccggat tgaggga
//
LOCUS       DAZFRN010000344         1817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFRN010000344 DAZFRN010000000
VERSION     DAZFRN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.347"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(1099..>1817)
                     /locus_tag="ACHKUB_005197"
     CDS             complement(1099..>1817)
                     /locus_tag="ACHKUB_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6658060.1"
                     /translation="PDILVLLETNLWWEQKLSPLDEVFPYSLKCPKENLYGMHVYSRM
                     ILSDAEIQYLVDKEIPSMHMIVHMPEGQRIRLHCLHPVPPSPTESDESTDRDSELLMV
                     GKSVADSELPVILAGDLNDVAWSATTRLFMKISGLLDPRRGRGFFSTFHSHYFFLRWP
                     LDHVFHSQHFLLKSLRRLPDMGSDHFPVMAELVYAPAHGMQQESLEKEKSDEVLAQKK
                     MDMTDSTAEDVHTPGGQKKS"
BASE COUNT          533 a          423 c          370 g          491 t
ORIGIN      
        1 ttaatattat taaagattct caccatcagt attcgcagtg aataatgcag cgtctcatat
       61 tcataccctg cagagaaaac ccctccgaag aggggcgagt gtagcgtaat ggagtgttat
      121 gtagcatggt gccgggtgcc tcccggtgag ttcagtatca gcacctgaac ccgcacagat
      181 aagggatgtg accaaacacc tacgatactg atatgccccg ccgctcaggg ggattcacca
      241 tgcattattt ttttaacaaa tatcccgtaa gaccgacaat aatccactga ctgaactgtg
      301 aagtatttaa catttaccga taaaaagctg cagctgcagc tgcagtggaa aaatacgaaa
      361 tcacatcatg cccgattgct gactcgcatc ctgccaatcg aacgtcgggc gaacaactga
      421 tcgaatacat cggaatccgc agacaaaaag cactgcagtc tcacgactgt gcgttatata
      481 tgtcagcgcc tgtaatataa gacggtaatg gggtcaaaaa tcgtggcgtt ggcatataca
      541 aaacaaaacg cccctccgta gaggggctga cgctgcgtat ctgtatcatc atgcgcatgg
      601 tgccgggtgc ctcccggtga acaaaccgtt cgtgatacca gtcagcaaca taggaaatta
      661 atggtatcac cccaccgcac agggggattc accatgcctg cctttttagc aaattcatca
      721 gaaaccggac aacatgctta tttatgaaat gaggtgcttt tcacatttat ataaaatcat
      781 tataacgata atcctaaaaa tcaatgtaat tatcatcttc agtaactttc tgtattacac
      841 catacggaaa ttgatttttg acggtaaaga tacaagggga agtatgtctg taaggagtac
      901 cctgaataat ggacaggcat gcatcgtggc tgttcccctg aaattatcag tacaaccagt
      961 cagctcataa tccctgtctc tttttccggg gagccctcag ggggactttc ccttatcccc
     1021 ccagtttctg gagagacgta aaaaagaatt aatcatatgt caggtatctg aatatgtccc
     1081 gtaattaata tatttacgtc aggatttttt ctgcccgcca ggagtatgaa catcttccgc
     1141 cgttgagtct gtcatatcca tttttttctg tgccagtact tcatctgatt tttctttttc
     1201 cagactctcc tgctgcatcc cgtgagccgg agcatataca agctcagcca tcactggaaa
     1261 atgatctgaa cccatatccg gtaaacggcg taaactttta agcagaaaat gctggctgtg
     1321 aaatacatgg tcaagcggcc atcgcaggaa aaaataatgg ctgtgaaacg tgctaaaaaa
     1381 accacgtccc cggcgtggat ccagcagtcc acttattttc atgaataaac gagtggtcgc
     1441 agaccacgca acatcattca gatcccccgc taatattaca ggtaattcag aatctgcaac
     1501 acttttcccg accattaaaa gttctgagtc acgatcagtt gactcatcac tttcagtcgg
     1561 gctcggtgga acaggatgaa gacaatgtaa tcttattctc tgaccttccg gcatatgaac
     1621 tatcatatgc atggagggaa tttccttgtc cactaaatac tgtatttctg catcacttaa
     1681 aatcatcctt gaatatacat gcatgccata taaattttct ttcgggcatt tcagggagta
     1741 tggaaacact tcatccagag gtgatagttt ctgttcccac cataagtttg tttcaagaag
     1801 aacaagtata tcaggtt
//
LOCUS       DAZFRN010000345         1794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFRN010000345 DAZFRN010000000
VERSION     DAZFRN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.348"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1291
                     /locus_tag="ACHKUB_005198"
     CDS             <1..1291
                     /locus_tag="ACHKUB_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6658061.1"
                     /translation="NGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQVAYNKYLVQTA
                     TRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYNDFYDIGRKNSLSANIMQPLSNNL
                     GNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEERFNLFIS
                     IPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQN
                     NDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWSGGINITNQLSDTFAI
                     LDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQGNRQII
                     APYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIR
                     VDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1304..>1794
                     /locus_tag="ACHKUB_005199"
     CDS             1304..>1794
                     /locus_tag="ACHKUB_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO6658062.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNS"
BASE COUNT          574 a          350 c          347 g          523 t
ORIGIN      
        1 aaatggttgg aatacaccgc tgggtgccat ctctttttac gctgcccgat cgagtagtaa
       61 gttaaataat gataccagac acgaaggtac gagctatcag gtcgcctata ataaatatct
      121 agtacaaaca gccactcgat ttagcgtggc cgcctggcgt tatgcttcgc aggattacag
      181 gacattcagc gaccatcttt acgaaaatga taaacactat catcagagtg attataatga
      241 tttttatgat attggcagaa aaaatagcct ttctgccaat attatgcaac ctttatccaa
      301 taatctggga aatgtatcat taagtgcgct ttggcggaat tactgggggc gaagtggaaa
      361 tgctaaagat taccaattca gttactccaa taactggcaa cacattagtt atactttctc
      421 tgccagccaa tcttatgatg aaaataataa agaagaggag cgttttaatc tgtttatctc
      481 cattcctttc tactgggggg atgatattgc caaaacacgt caccaaatta acttatcgaa
      541 ttcgacctca ttttccaaag atggctattc ctccaacaat actggaatta ctggcatagc
      601 cggtgaacat gatcagttaa attatggtat atatgttaat cagcaacaac aaaataatga
      661 tacctcgctt ggtacgaatt taagctggag aactcccatc gccataatag atggcagcta
      721 tagtcattct aaaaacgcct ggcaaagtgg tggaagtatt agtagtggat tagttgtctg
      781 gtccggtggt attaatatca ctaaccagtt atccgataca tttgcaattc tggatgcgcc
      841 tggattagaa ggtgcacata ttaatggaca aaaatacaat cgaacaaaca gcaaaggcca
      901 ggttgtttac gacctgataa tacctcatcg tgaaaaccat ctggtacttg atatagcaaa
      961 cagtgaaagt gaaacagaat tgcagggcaa tcgtcaaatt attgcacctt accgtggagc
     1021 agtttcttat gtgcagttta caactgacca acgtaagccc tggtatatac aggcactgcg
     1081 tcccgatggt tcgccattaa cctttggcta tgacgtactg gatctccagg aaaataatat
     1141 tggagtcgtt ggccagggta gtcgcctttt tattcgcgta gatgaaattc ccactggaat
     1201 aaaagttgct ctcaatgatg aacagaactt attctgtact attacttttc aacacgttat
     1261 cgatgaaaac aaaacatata tatgccagta aaaggagttt actatgaagt ggcttttatt
     1321 aatcaccttg agtctatatt cttttattgt gcaaagtgct ccctgtgcat tgaccaatgt
     1381 cggggaacaa cgtggaacat atatactcaa gccactgagt atgaaaggta atctcaccgc
     1441 cgaaaaaatt gaaaacaccg gaaatatcat tgagtgtgca gacttacaag agacgaccaa
     1501 cgccattaaa gttcattttt taacacagaa cgctttaagg tttaaagtcg gaagtcgtca
     1561 ttatattatt aatttcccct ttgaatctga cggtaaaaaa atagagcttc caggaaggtc
     1621 atcttataat ttagaagaaa tgctatctgc gataaatttc accattccat actctatcgc
     1681 atctgtatca gatagttcca acaccgcaga tatttttcca ggaactcctt ttcctttgtt
     1741 gttcgaaatt gaagtgagtt catgtacagg gaataatact aattgtaata gcat
//
LOCUS       DAZFRN010000346         1775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFRN010000346 DAZFRN010000000
VERSION     DAZFRN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.349"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1775
                     /locus_tag="ACHKUB_005200"
     CDS             <1..>1775
                     /locus_tag="ACHKUB_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO6658063.1"
                     /translation="NPFPASLAAQNTNVVEVDETDKGHQVYGDNWKSIEIGTTKEVKK
                     KDSTDLNFDHNSPVAKGHNSIAIGTSAEAGGEIKDEKGFKTGESIAIGAKAKATKEQS
                     IAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKKIPNDYSSSEASGDGSIVVGSRSTA
                     AGDLSVSLGSISSANGTAANALGATAEANGDMATAVGFNALANASNALAVGSQASAGG
                     EDSLAIGSQSTTGKKSTVALGQGATASAAEGNVAIGADSVDKAATPVSGATIKLKNGG
                     TIEYRGFAGDKAASVVSVGDAGKERQIVNVGAGAVSEKSTDAINGSQLYAIAKTLKDD
                     LDAINPTWKLKDSNGGSKEVGSKNGGTTGNEVTVTGKGDIDVTVDGNGLSIDGTKLAE
                     NIKKDAIKIKTGDGKTLTKKLGEEVEFNDAGPIKTKINNNKLEITAETASFTPDGNGG
                     NGKITVSNGDDDKLVTAKKIADAINQSGWKIKAGSENGGKVEGDNKEELINPGDTVTL
                     KAGKNLSVKQSNGDFTFSVVDTPSFSSVQLNGKGNNGSVMLSSEDNKLTLSGGNGAGQ
                     NGVVISGVASGLNNKDLAGLKADDA"
BASE COUNT          563 a          309 c          505 g          398 t
ORIGIN      
        1 aatccgttcc ctgcatcact ggcagcacaa aacactaatg tagttgaagt tgatgaaaca
       61 gataaaggtc accaagttta tggtgacaac tggaaaagta ttgaaatagg tacaacgaag
      121 gaagtaaaga aaaaggattc aaccgacctt aattttgacc ataactcccc ggtagcgaaa
      181 gggcataact ccattgcgat tggtaccagt gcagaagcag gaggtgaaat taaagacgaa
      241 aaaggtttta agacaggaga atccatagct attggtgcca aggcgaaagc aactaaagaa
      301 cagtcaattg caataggtgg cgataccaaa gctaccggat ggggggctat tgttattggt
      361 ggcgatgatt taaccccttt gcatggtgac agattcggtg ataaaaaaat tccgaatgat
      421 tactcgtcat ctgaagcatc aggagacgga agtattgttg tgggctcaag gagcacagct
      481 gcaggagacc tgtctgtgtc cctgggctca ataagctctg ccaacgggac ggctgctaat
      541 gcactgggag ccacggcgga agctaacggg gatatggcga ctgcggttgg ttttaatgcg
      601 ctggctaatg ccagtaatgc gctggctgtt ggtagtcagg cctctgccgg cggagaggat
      661 tccctggcta tagggagtca gagtacgacg ggcaaaaagt ccacggttgc tctggggcag
      721 ggagctacag cctctgccgc ggaggggaac gtggccatcg gtgctgattc agtggacaag
      781 gcagcaactc cggtttccgg cgctaccatt aagttaaaga atggcgggac tattgaatac
      841 aggggctttg ccggagataa ggctgcatca gtagtatctg ttggtgatgc gggtaaagag
      901 cgtcagattg ttaacgtggg ggcgggtgct gtatctgaga aaagtacaga tgccattaac
      961 ggtagtcagt tatatgcgat agcgaagaca ctgaaagatg accttgatgc cattaatccg
     1021 acgtggaagt taaaagacag taacggtgga agtaaagaag tcggcagtaa aaatggcggg
     1081 actacaggta atgaagtgac cgtcaccggt aaaggggata ttgatgttac tgttgatgga
     1141 aatgggctga gtattgatgg cacaaagctt gctgaaaaca tcaaaaaaga tgccatcaag
     1201 attaaaacgg gagatggtaa aacactaacg aaaaaacttg gtgaagaagt tgagtttaat
     1261 gatgccggac caataaaaac aaaaattaat aataacaaac ttgagataac tgccgaaaca
     1321 gccagtttta cgcctgatgg aaatggtggt aacgggaaga ttacagtaag caatggtgat
     1381 gacgataagc ttgttactgc gaaaaagatt gctgatgcca taaaccagtc tggctggaaa
     1441 attaaagctg gcagtgaaaa tggtgggaaa gttgaggggg ataataaaga ggagctaatt
     1501 aatcccggtg atacagttac actgaaagcc ggtaaaaatc tcagcgtgaa acaaagtaac
     1561 ggtgatttta cattctctgt tgttgatact ccgtcattca gcagtgttca gctgaatgga
     1621 aaaggaaata acggaagtgt aatgctctca tccgaagata ataagctgac attgtcaggc
     1681 ggtaatggtg ccggacaaaa tggtgttgtt atcagtggtg tggcaagtgg attgaataat
     1741 aaggacctgg caggcctgaa agcagacgat gcgga
//
LOCUS       DAZFRN010000347         1765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFRN010000347 DAZFRN010000000
VERSION     DAZFRN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.350"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(22..390)
                     /locus_tag="ACHKUB_005201"
     CDS             complement(22..390)
                     /locus_tag="ACHKUB_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6658064.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWRLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(553..774)
                     /locus_tag="ACHKUB_005202"
     CDS             complement(553..774)
                     /locus_tag="ACHKUB_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6658065.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(837..1313)
                     /locus_tag="ACHKUB_005203"
     CDS             complement(837..1313)
                     /locus_tag="ACHKUB_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6658066.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(1329..>1765)
                     /locus_tag="ACHKUB_005204"
     CDS             complement(1329..>1765)
                     /locus_tag="ACHKUB_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6658067.1"
                     /translation="ASGNDQPQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVFGWM
                     DRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAGIAA
                     CLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT          395 a          461 c          522 g          387 t
ORIGIN      
        1 gcagaaggtt attccggtgg tttacttttt cgtttcgggc gtcggataaa cagccagata
       61 aacgtaacca ccgctaccga gagtgtcagc tttgcaggtc agaccttttg catacagcgt
      121 gacggtatgc tgatggcggg gattcagttc accgctggtg agcatgagtt ccagttgttt
      181 catcagcagc ggaaaggcct ggtccaggtg gtacgcatct gcatcgctga acaggcctct
      241 gataccggcg cggtcggcaa ggtaatgcag gcggttaccc tcctgcacca gacgggcgcc
      301 gaaacagggc gtcactgtgc agggtagtct ccaccaggga tggtcgtcat tgtcatcggg
      361 atgcgttgtc ccggaaagtg tgtctgacac gataaaatcc tcacagaaaa tcggtgaatg
      421 atagtttaac gatgacgggt cagccggcgc gcagaccggc ttcggtaaag gtgtattcgt
      481 tgatgagcag gacctcatcc acagcggcgt ctgagctcag ccagtcgtac tcgttttccg
      541 ggtgacggaa catcagttca gggtgacgct catcagccgg acatacggac caaaactgtc
      601 cttacggcgt tctgcaaata cggccagcac accgggaata tcctgcactt cacgaccggt
      661 atacgcttca gcactgccgt gccagcggta tttgccggta cagaacggaa aaagacggga
      721 tgccggatgc tgacggtgaa tacgcatggc ttcgccacgg gtgatgattt tcatagtggg
      781 atacctctga agacagaaga taaaagtgaa aacaggtgtg atgtggttgt gacgggttaa
      841 agcagaccat gttcggcaaa ggagaaaacc tggctgccac cgactatcag atggtccggc
      901 acccggatat ccaccaggcc cagtgcctgt accagacgtt ccgtgataag gcggtctgcc
      961 ttgctgggtg tgacttcacc ggacgggtga ttgtgtgcca gcaccacagc agcggcattg
     1021 tgatacaggg cgcgtttaat cacttcacgg ggatggactt ccgtgcggtt gatggtgccg
     1081 gtgaagaggg tttcaccggc aatcagctga ttctggttgt tcagatacag cacccggaac
     1141 tcttcacgct ccagtcccgc catgttcaga atcagccatt ctcgtgccgc acgggtggag
     1201 gtgaaggcca cgccgggttc atgaagatgg cggtccaggg ttttcagggc ccgttgaatg
     1261 aggctgcgct cgccgggcgt catctctccg ggcagaaagg aaagctgttg cattgtactt
     1321 ctctccattc agtcgatgat gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa
     1381 tcccgcagcc ggtaataatg gaccgtcatg gcataacact ccgtacgaca ggcatgatga
     1441 ctgtacgtca tcagacaggc agcaatgccg gcagcttccg gactcatttc agcgcggtta
     1501 ccgttcatgg cattgaacag tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc
     1561 ataaatgccc cgccgttgtt cagggtgtac agattccaga taccaccgca gtagtcttca
     1621 cacagacggt ccatccagcc gaagacacgg ggctccaggg tcacccactg tggaatgagg
     1681 ccaaagtgct gcggccagaa gctgatgcgc tgttcatcag ggacgagggt ggcaaccaac
     1741 tgaggctggt cattccctga tgcag
//
LOCUS       DAZFRN010000348         1745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFRN010000348 DAZFRN010000000
VERSION     DAZFRN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.351"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..1011
                     /locus_tag="ACHKUB_005205"
     CDS             <1..1011
                     /locus_tag="ACHKUB_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6658068.1"
                     /translation="NQYLESLRLNTYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKN
                     VSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDR
                     NNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFT
                     ATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLS
                     VDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVK
                     DKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCIS
                     QN"
     gene            1027..>1745
                     /locus_tag="ACHKUB_005206"
     CDS             1027..>1745
                     /locus_tag="ACHKUB_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6658069.1"
                     /translation="MRYLNTKNVIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNS"
BASE COUNT          559 a          367 c          362 g          457 t
ORIGIN      
        1 aaccagtatc ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac
       61 tgggacgcca gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggg
      121 ccattaaaaa acgtctccac ttctctaaca tttagccgaa taaactggga agaagacaac
      181 caggatcaac tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat
      241 ggtatgcaac gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc
      301 gatcgaaata attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg
      361 aaagcgtcac tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc
      421 gggacatcac agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg
      481 gcgactcgtc acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg
      541 atcgacgcag atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg
      601 tatggcatcg gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat
      661 acccgaaatc ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc
      721 gaaggtgcta ttggctacgc caaacttgat acccgcaagg gctatcaaat catgggggtt
      781 attcgcctgg cagatggtag tcacccacca ctggggatta gcgtaaaaga taaaaccagc
      841 cataaagaat taggactggt tgctgatggc ggctttgtat acctcaacgg cattcaggat
      901 gacagcaaac ttactttacg ctggggtgat aaatcctgtt ttattcaacc acccaatagc
      961 agcaacttaa ccaccggaac ggttatttta ccgtgtatta gccaaaatta attcaggatg
     1021 aaactaatgc gctacttaaa cacaaagaat gtcattgcag caggagtatt actcgcctgc
     1081 atgagcagca ttgcctgggg tgccatcatc ccggaccgta cccgtatcat aatgaatgag
     1141 tcagataaag gggaagcatt aaaacttact aatcaaagca aaaatctacc ctatctggcg
     1201 caaacatgga tagaagatac gaaaggcaat aaatcacgtg actttattgt cacggttccc
     1261 cctatggtac gtttaaatcc cagtgagcaa attcaaataa gaatgataag tcaggaaaaa
     1321 attgctcaac tgcctaatga cagagaaacg ctcttctatt ttaacgtgcg agaaataccg
     1381 ccaaaaacag ataaaaaaaa tgtgatgcaa gtcactatgc agcacgcatt gaaactattc
     1441 tggcgaccaa aagctattga attagaagat gatggggtaa tgtcttacga aaaagttgaa
     1501 atcatccgta gaaatgatgg ttcgatccgc tttaacaata agatgcctta tcacgttacg
     1561 ctgggttata tcggaacaaa cggaatcaca atgttgccac aaacgcaaag tttaatggta
     1621 accccttata gccatgcaga tacgcagttt aaaaatgttc cgtcagcttt tcaggttggt
     1681 tatatcaatg attttggtgg attaagtttt tatgaaataa actgcccgac ggttaataac
     1741 tcttg
//
LOCUS       DAZFRN010000349         1724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFRN010000349 DAZFRN010000000
VERSION     DAZFRN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.352"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1724
                     /locus_tag="ACHKUB_005207"
     CDS             <1..>1724
                     /locus_tag="ACHKUB_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6658070.1"
                     /translation="GQWINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNG
                     LAVNTTLDNRGEQWVHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENV
                     SSGQMVGGTAESTTINKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYV
                     HNGGTATETLINRDGWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTS
                     SAATVTGTNRLGAFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGA
                     ATTVSMGNGGVLLADSGAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATD
                     TTVNGGLFTARGGSLAGTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGR
                     VEKSGSGTLTVSNTTLIQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGA
                     IDPTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQ
                     NGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAI
                     NGATTEEGA"
BASE COUNT          435 a          461 c          539 g          289 t
ORIGIN      
        1 gggcaatgga taaacgcggg tggcacaggc agaaacacca ctgtcaccgc aaatggtcgt
       61 cagattgtgc aggcaggagg aactgccagt gatacggtta ttcgtgatgg cggagggcag
      121 agccttaacg gactggcggt gaacaccacg ctggataaca gaggtgagca gtgggtacac
      181 gggggaggga aagctgccgg tacaattatt aaccaggatg gttaccagac cataaaacat
      241 ggcggactgg caaccggaac catcgtcaac accggtgcag aaggtggtcc ggagtctgaa
      301 aatgtgtcca gcggtcagat ggtcggaggg acggctgaat ccaccaccat caacaaaaat
      361 ggccggcagg ttatctggtc ttcggggatg gcacgggaca ccctcattta cgccggtggt
      421 gaccagacgg tacacggaga ggcacataac acccggctgg aggggggtaa ccagtatgta
      481 cacaacggtg gcacggcaac agagacgctg ataaaccgtg atggctggca ggtgattaag
      541 gaaggaggaa ctgccgcgca taccaccatc aaccagaaag gaaagctgca ggtgaatgcc
      601 ggcggtaaag cgtctgatgt cacccagaac acgggcggtg cactggttac cagttcagct
      661 gcaaccgtca ccggtacaaa ccgcctggga gcattctccg ttgtggcggg taaggctgat
      721 aatgtcgtac tggaaaatgg cggacgtctg gatgtgctga gcggacacac agccactaat
      781 acccgtgtgg atgatggcgg aacgctggat gtccgcaacg gtggtgccgc caccaccgta
      841 tccatgggga atggcggtgt actgctggcc gattccggtg ccgctgtcag tggtacccgg
      901 agcgacggaa cggcattccg tatcggaggc ggtcaggcgg atgccctgat gctggaaaaa
      961 ggcagttcct tcacactgaa cgccggtgat acggccacgg ataccacggt aaatggcgga
     1021 ctgttcaccg ccagaggggg ctcgctggcg ggcaccacca cgctgaataa cggtgccacg
     1081 cttacccttt ccgggaaaac ggtgaataac gataccctga ccatccatga aggtgatgca
     1141 ctcctgcagg gaggcgctct taccggtaac ggcagggtgg aaaaatcagg aagtggcaca
     1201 ctcactgtca gcaacaccac actcatccag aaaaccgtca acctgaatga aggcacgctg
     1261 acgctgaacg acagtaccgt caccacggat gtcatcgctc agcgcggcac ggccctgaag
     1321 ctgaccggca gcactgtgct gaacggtgcc attgacccca cgaatgtcac cctgacatcc
     1381 ggtgccacct ggaatatccc cgataacgcc acggttcagt cggtggtgga tgacctcagc
     1441 catgccggac agattcattt cacctccgcc cgcacaggga agttcgttcc gacaaccctg
     1501 caggtgaaaa acctgaacgg gcagaatggc accatcagcc tgcgtgtacg cccggatatg
     1561 gcgcagaaca atgctgacag actggtcatt gacggtggca gggcaaccgg aaaaaccatc
     1621 ctgaatctgg tgaacgccgg caacagtggt acggggctgg cgaccaccgg taagggtatt
     1681 caggtggttg aggccattaa cggtgccacc acggaggaag gggc
//
LOCUS       DAZFRN010000350         1724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFRN010000350 DAZFRN010000000
VERSION     DAZFRN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.353"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1724
                     /locus_tag="ACHKUB_005208"
     CDS             <1..>1724
                     /locus_tag="ACHKUB_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6658071.1"
                     /translation="GQWIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNG
                     LAVNTTLNNRGEQWVHEGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNS
                     YTGQKVQGTAESTTINKNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYV
                     HKDGLALNTVINEGGWQVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTS
                     TAATVTGINRLGAFSVVEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGT
                     ATTVSMGNGSVLLADSGAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATD
                     TTVNGGLLTARGGSLAGTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGR
                     VEKSGSGTLTVSNTTLTQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGA
                     IDPTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQ
                     NGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAI
                     NGATTEEGA"
BASE COUNT          435 a          466 c          532 g          291 t
ORIGIN      
        1 gggcaatgga tacagaatgg cgggatagcc ggaaacacca ctgtcaccac aaatggtcgt
       61 caggtcgtgc tggagggggg aacagccagt gatacggtta ttcgtgacgg cgggggacag
      121 agcctgaacg ggctggcggt gaacaccacg ctgaataaca gaggcgagca gtgggttcat
      181 gagggcgggg ttgccaccgg tacaattatc aaccgcgacg gttaccagag cgttaaaagt
      241 ggcgggctgg caacaggaac catcatcaac accggcgcag aaggcggccc ggattctgac
      301 aactcgtaca cgggtcagaa ggtccaggga acagcagaat ccaccaccat caacaaaaat
      361 ggacggcaga ttatcttatt ttccgggata gcccgtgaca ctctcattta cgcaggtggt
      421 gaccagtcgg tacacggaag ggccctgaat accacactga atggcggtta ccagtatgtg
      481 cacaaggacg gacttgcgct gaacacggta attaacgagg ggggctggca ggttgttaag
      541 gcaggtggtg ctgtcggtaa caccaccata aatcagaacg gtgaactgag ggtacatgcc
      601 ggcggggaag ccactgcagt cacccagaac acgggcggtg cactggttac cagtacggct
      661 gcaaccgtta ccggcataaa tcgccttgga gcattctcgg ttgtggaggg taaagctgat
      721 aatgtcgtac tggaaaatgg cggacgcctg gatgtgctga ccggacacac agccactaat
      781 acccgcgtgg atgatggcgg aacgctggat gtccgcaacg gtggcaccgc caccaccgta
      841 tccatgggaa atggcagtgt actgctggcc gattccgggg ccgctgtcag tggtacccgg
      901 agcgacggaa cggcattccg tatcggaggc ggtcaggcgg atgccctgat gctggaaaaa
      961 ggcagttcat tcacgctgaa cgccggtgat acggccacgg ataccacggt aaatggtgga
     1021 ctgctcactg ccagaggggg ctcgctggcg ggcaccacca cgctgaataa cggtgccacg
     1081 cttacccttt ccgggaaaac ggtgaataac gataccctga ccatccgtga aggtgatgca
     1141 ctcctgcagg gaggcgctct taccggtaac ggcagggtgg aaaaatcagg aagtggcaca
     1201 ctcactgtca gcaacaccac actcacccag aaaaccgtca acctgaatga aggcacgctg
     1261 acgctgaacg acagtaccgt caccacggat gtcatcgctc agcgcggcac ggccctgaag
     1321 ctgaccggca gcacagtgct gaacggtgcc attgacccca cgaatgtcac cctgacatcc
     1381 ggtgccacct ggaatatccc cgataacgcc acggttcagt cggtggtgga tgacctcagc
     1441 catgccggac agattcattt cacctccgcc cgcacaggga agttcgttcc gacaaccctg
     1501 caggtgaaaa acctgaacgg gcagaatggc accatcagcc tgcgtgtacg cccggatatg
     1561 gcacagaaca atgctgacag actggtcatt gacggtggca gggcaaccgg aaaaaccatc
     1621 ctgaacctgg tgaacgccgg caacagtggt acggggctgg cgaccaccgg taagggtatt
     1681 caggtggttg aggccattaa cggtgccacc acggaggaag gggc
//
LOCUS       DAZFRN010000351         1716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFRN010000351 DAZFRN010000000
VERSION     DAZFRN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.354"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            10..1707
                     /locus_tag="ACHKUB_005209"
     CDS             10..1707
                     /locus_tag="ACHKUB_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO6658072.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
BASE COUNT          553 a          373 c          343 g          447 t
ORIGIN      
        1 gaataccata tggaacatca gttaaataaa aaactgctta actcatcaat atcacttata
       61 ttagcatctg gtctgatgat gagtactgca aatgcagcag atgatgttaa gctcaaagca
      121 acaaaaacca atgttgcctt tgcggacttc acgccgacgg aatacagcac taaaggtaag
      181 ccaaatatca ttgttttaac aatggatgat ttagggtatg gacaattgcc ttttgatgat
      241 agttcatttg accctaaaac gatggataac cgaaatgttg ttgatacata caaaataggt
      301 attgataaag caattgaagc tgcaaaaaaa tccactccaa cattagcggc cctgatggac
      361 gaaggtgtaa aattcactaa cggctatgtt gcacacggtg tctctggccc ttcacgtgcc
      421 gcaatcctga cggcgcgagc tcctgctcgc ttcggtgtat attcaaattc tgatgcacag
      481 gatggtatcc ctctggaaga aacattcctg tctgaattac ttcagaatca tggttattac
      541 actgcagcaa tcggtaagtg gcacttatca aaaatcagca acgtaggtgt gcctgaagaa
      601 aaacaaaccc gtgactacca cgataacttc accaccttct ccgcagaaga atggcaacct
      661 cagaatcgtg gcttcaacta cttcatgggt tatcacgcat ccggcgtttc actttataac
      721 tccccgtcat tgttccgtaa ccgtgaaaac attaaagctg aagggtacat tagtgatcaa
      781 ttaactgatg aagcaattgg tgtaattgac cgtgcaagca aactggaaca gccatttatg
      841 atttatctgg catacagtgc accacacctg cctaacgatc agcctgcgcc taaaaactat
      901 caggagaaat tcaacactgg tagcaagact gctgataact attatgcgtc aatttactcc
      961 gttgaccagg gggttaaaaa gatcctgaat acacttaagg aaaaaggtca gtacgacaac
     1021 accatcattc tgttcacctc tgataacggt gctgtaattg atggtccact gccactgaat
     1081 ggtgcgcagg ctggttacaa aggccaaccg tatccgggtg gtatgcatac acctatgttc
     1141 atgtggtgga atggcaagct gaaaacgggt gattatgaca aactggtttc atcaatggac
     1201 ttctatccga ccgctttaga tgccgcagat atcaagatcc ctgcttctct gaaactagat
     1261 ggtgtatcat tattgccata tgtacagaaa ggagctgata agaaatctgc agctggcgaa
     1321 cctcacaaat atctgtcctg gattacctct tattctcact ggtgggacga agagaacact
     1381 gcattctgga acggatatca taaattcgtg agaaaagagt ctgatgatta tccgcacaac
     1441 ccacatacag aagacttaag ccagtttgca tacactgtaa gaagcaacga ttattctctg
     1501 gtttacacct atgcagagaa taaactagct ctgtacgata tcaacgatct gcaacaaact
     1561 aaagatctct ctgcatccca tcctgatgta gttaagaata tgaaggctgt tatgattgac
     1621 ttcatcacgt caagtcaaaa accgatcagt gatatcaacc aagccaaatt tgagaatata
     1681 aaaaaatctt taactaacgt gaaatgataa taagca
//
LOCUS       DAZFRN010000352         1686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFRN010000352 DAZFRN010000000
VERSION     DAZFRN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.355"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(73..270)
                     /locus_tag="ACHKUB_005210"
     CDS             complement(73..270)
                     /locus_tag="ACHKUB_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6658073.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKREVEQWK"
     gene            complement(338..608)
                     /locus_tag="ACHKUB_005211"
                     /pseudo
     CDS             complement(338..608)
                     /locus_tag="ACHKUB_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(658..1224)
                     /locus_tag="ACHKUB_005212"
     CDS             complement(658..1224)
                     /locus_tag="ACHKUB_005212"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6658074.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGTIERHLGCSDF"
BASE COUNT          454 a          423 c          387 g          422 t
ORIGIN      
        1 aatactaatc ctgaacaata ctgcagcccc ccccttgatc actcgattcg ttgctacatc
       61 cactgctcta cttcacttcc actgctccac ttctcttttg taccagcggc tgctgcgccc
      121 cagtttgatg ggtttaggga aatggccttc actgatcaac ttgtaaaacc atttgtcagt
      181 cataccggtg aaactggtga tatagttcat gtcgatcagg gaattatcgg cacgcagaaa
      241 tacgctgtcg tggcttgttg tagtcaacac ggtcagaatg tcccctgtga gatttatgaa
      301 gattgtgcga atgcggggaa tctgctgagc tgtgctttca gaactggcct gttacgggat
      361 accagggatt accggcgagg taacgggctt ccggatcata cacacctcga tgatcgcgga
      421 caaaatcact gaacgcccat atcacctctt taagtatgtc ttcgcagtcc ggtaaatgac
      481 gatccagcgc cacatcccgg gtggtactgc tttgatgcgc ccggtgacac aaagcccgga
      541 ttattccaga catcctgaat caaacgcccc agattagggg cgtcgaaata tgcctctctg
      601 accattatat tccggcgtac aggtagcagg tcagaagtga caatgcgtca cctgacgtta
      661 aaagtcacta cacccaagat gacgttcaat ggtaccatgt gattcaatgt aagcccgggc
      721 cgtctgttcc agtacaccag gctcagcgtt gtatgtgtta gctgcatcaa ataccaacga
      781 cagcacttca ggatacacaa ccagatgtgt aatggagtta tcttcaccca atacttttcc
      841 ccacgcctgc tcaatcagat ttctgagaac caccacctca cgactcttac accagacatc
      901 gttattaagt agcagcacca taagataagg agtggtatcg ttagccacag cttccctact
      961 ccagagataa tataaagggg tgggctcaac agattgatct ttacgtcgct tacactgcaa
     1021 atattcagaa atgagtctat gcagttcacc agtaaaatca gccatcagag agggaatggc
     1081 cttattaata ccctgccctg gtattaattt aaattgtaat aatttaattt caggatgtgt
     1141 ggctgcagcc cgatacagag ttgcaaggac acacttttgc cagagggcgt tacaggaaag
     1201 cttaacattt gattctgtat acataataaa tcaccttaca gttacaacag gtcaaaaaac
     1261 gctgtagcca gagttacgct ggcctgatgc tttagtaccg ggcatcgtct gataatccag
     1321 acgctccaat aagcgctgat actgctcagg gaaatcagga tcatgaatat cctggatgtc
     1381 acgtccatta gcagggaaat aaacaatgca gccccctgga ttaactatgc agaaatcgtc
     1441 ctgaggcact gataaatacg gagaggactc tcgcgtatgg ttggttgaca ccacagtgcg
     1501 gattcggcga atccgcgatc acggtgcgat ttcgcaccac agtacacaat caataccccg
     1561 ggttttattc agggtaagcg tacagcgagg gccgtattga cggggatgtg ttattcagct
     1621 ggcagtgcta tgcgccacgg aagcagttcg ctgacccggt tgaccggcca gtctgctatg
     1681 acgcca
//
LOCUS       DAZFRN010000353         1675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFRN010000353 DAZFRN010000000
VERSION     DAZFRN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.356"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            123..731
                     /gene="yiiX"
                     /locus_tag="ACHKUB_005213"
     CDS             123..731
                     /gene="yiiX"
                     /locus_tag="ACHKUB_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO6658075.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGNNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            876..1551
                     /locus_tag="ACHKUB_005214"
                     /pseudo
     CDS             876..1551
                     /locus_tag="ACHKUB_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          439 a          422 c          391 g          423 t
ORIGIN      
        1 atattgcaat ttattgaatt tgcacgaact tgtaggccgg ataaggcgtt cacgccgcat
       61 ccggcataaa caacgagcac gttgtctgcg acccaccgct ttttatacat ggacgtttaa
      121 ctatgaaaaa caggctgctg atcctcagcc tgctggtttc tgtacctgcc tttgcctggc
      181 agccgcaaac tggcgacatc atctttcaaa tctctcgctc atcgcaaagt aaagcgatcc
      241 aactggcaac ccattccgat tacagccaca ccggtatgct ggtgatacgc aacaaaaagc
      301 cctacgtttt tgaagcggtc ggcccggtga aatacacccc gctcaagcag tggatcgccc
      361 atggtgaaaa gggcaaatac gtcgttcgcc gcgttgaagg cggactgagt gttgaacaac
      421 agcaaaaact ggcgcaaacg gcaaaacgtt atctcggtaa accgtatgat ttcagcttct
      481 catggagcga cgatcgccag tactgttcgg aagtggtgtg gaaggtttac cagaacgcgc
      541 tgggaatgcg cgtgggcgaa cagcagaagt tgaaagagtt tgatctcagc aacccgctgg
      601 tccaggcgaa gctcaaagaa cgctacggta ataatattcc gcttgaggaa acggtagttt
      661 caccacaagc cgttttcgac gcgccacaac tcaccacggt tgccaaagag tggccgctgt
      721 tttcgtggta atggatgatg aaaaaaataa ttcccgccgt agtctgtttg ttgctttgta
      781 gttgcacgac ctttccgcaa gctgtcgctc cggtaaacgg tgatgcctcc aattagttga
      841 attaaggata taatactcgc ttttaaggtt gtttcatggt cacagttaac cttcattgcc
      901 cccgttgcca gtccgttcag gtttaccgtc acgggcagaa cccaaaaaga catgatcgat
      961 tccgcggccg tgactgtcac cgggtatttc agctcagtta ctgttacgag ccccgtaaac
     1021 cgggagttaa agaccaaatc gctgaaatga ctttcaacgg tgccggggtt cgcgatactg
     1081 cccgtacgct gaaaatcggc attaatacag tcatccgaac tttaaaaaac tctcgccacg
     1141 aagaataacg tcatccccgg tcgctcatgc tgatgtggca ctcatctgca aacttagtca
     1201 gcaatggagc tttgtcggca gtaaagcgcg acagcactgg ctctggtacg tgtacaacac
     1261 gaaaactggt ggtgttctgg cctacacttt tggcccccgg actgacgaaa cctgtcgtga
     1321 attgcgggcg ctgcttaccc ttttaccatc ggcatgttga ccactgacga atggggtagt
     1381 tacgctagaa agttaccgaa ggagaagcat ctgactggta agatttttac tcagcacatt
     1441 gagcgtaaca atctgaccct gagaacccga atcaaacgtc tggcccgcag aaccatctgt
     1501 ttctcgcgtt ctgtagaact tcatgaaaaa gtcatcggtg cttttatcga aaatatatgt
     1561 tctactaatt ggagtcatca cccttctgat aagctttcct tggatgatta ccagactgaa
     1621 tacacagcca aagattttta cctcattctg ctcttttttc tcaaatgaca tatat
//
LOCUS       DAZFRN010000354         1638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFRN010000354 DAZFRN010000000
VERSION     DAZFRN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.357"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..1240)
                     /gene="yiaN"
                     /locus_tag="ACHKUB_005215"
     CDS             complement(<1..1240)
                     /gene="yiaN"
                     /locus_tag="ACHKUB_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO6658076.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFI"
     gene            complement(1243..>1638)
                     /gene="yiaM"
                     /locus_tag="ACHKUB_005216"
     CDS             complement(1243..>1638)
                     /gene="yiaM"
                     /locus_tag="ACHKUB_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO6658077.1"
                     /translation="RYGFQTSILSVDELSRYLFVWLTFIGAIVAFMDNAHVQVTFLVE
                     KLSPAWQRRVALVTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPT
                     SLVIAFFELRHLYQLITRSNSLTSPPQGA"
BASE COUNT          508 a          416 c          406 g          308 t
ORIGIN      
        1 gaataaaaac aaatacgact aataatgaat ataaaaccag gacgtaaggg aaaacgcctc
       61 tgactgcatc atcgaatttg agttttgcca cccctgaaat aacgttaagt acattaccga
      121 taggcggtgt aattaagccg attgagcagt taatgataaa catgacaccg aaataaatcg
      181 gatcgattcc tgcctcttta actaaaggca ttaataccgg ggtaagaatt aataccgtcg
      241 gcgttaaatc catgaccatg ccgacaatca ggatagccac cataatgacg ataaacagca
      301 gacgcggtga atcgaccaac ggttgcagta aatcagaaac catcatcggc agttcagcaa
      361 tggtaatcag ccaggcggaa acttgtgccg aggccaccag aaacataacc actgaggtgg
      421 ttttcgccgc gccaatgagt acatgccaaa gtgtggcgaa ggtcatttca cggtaaataa
      481 ctgtggcgac aaacagcgca tagaaagcag caacggcccc ggcttcggta ggggtaaaca
      541 gaccggagcg aaacccgcca ataatgatga ctggaagaaa cagcgcccag ataccggaga
      601 caaaagagtg ccagatttcc tgcatcgttg ctttttgctg gcggggtaaa ttaaggcgac
      661 tggcctgcca ccaccaggta agcatcagcg ttgcgcccat catcatgcca ggggcaatgc
      721 ccgccataaa cagcttactg atggataatc cgctggaaac gccgaagata ataaacggaa
      781 tggaaggggg aataattggc gcgataatgc cgccagaggc aatcagcccc gccgcccggt
      841 tgaccgggta gttggcgctg cgcatcatcg gcaccagtag agcggcaacg gcggcggtat
      901 ccgcaacggc agaaccagaa aggctcgcca taatcatagc tgccagcacg ccgacgtagc
      961 ccagcccgcc tggtttatgc cccaccagtt tcatcggcag gtcaacaatg cgttttgaca
     1021 agccgcccgc attcatgatt tcacccgcca gcacaaagaa cggaatcgcc agcagggaga
     1081 agctatcggc tccgttcacc agcgtttgtg ccatgatctg gacatcaaac atgtccagcc
     1141 agaacattaa cgccgccccg cacaacaaca gtgcccaggc aataggcaaa ccgatagcaa
     1201 taccacccaa cagacagccc agaaaaatca gcacagccat gattaagctc cttgcggtgg
     1261 cgacgttaga gaattgctac gcgtgatgag ttgatataaa tgacgcagtt caaagaatgc
     1321 gataacgagg ctggtgggca gacaagcggc atacatcagg ccgatgggta aaccgaggat
     1381 cggtgaataa tcgctccagt cctgaattgt ttttagcgtt gcgccccagg ccagtgcgcc
     1441 acaaataaat aagattaagg aatgggtaac cagagcgact cgtcgttgcc atgcggggga
     1501 gagtttctcc accagaaagg tgacctgaac gtgggcgtta tccataaaag ccacaatcgc
     1561 gccaataaat gttaaccaga caaataaata acgtgacaat tcatctacag ataaaatgct
     1621 tgtctgaaaa ccatatct
//
LOCUS       DAZFRN010000355         1633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFRN010000355 DAZFRN010000000
VERSION     DAZFRN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.358"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            348..962
                     /gene="evgA"
                     /locus_tag="ACHKUB_005217"
     CDS             348..962
                     /gene="evgA"
                     /locus_tag="ACHKUB_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO6658078.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            967..>1633
                     /locus_tag="ACHKUB_005218"
     CDS             967..>1633
                     /locus_tag="ACHKUB_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6658079.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISR"
BASE COUNT          517 a          327 c          319 g          470 t
ORIGIN      
        1 acacagtgca tgtgttgcac attatcccag gaacgttgga ggcctaattt aaatgaggtg
       61 caaaaaaaaa caacagagga ctaaaccgtg gcttttgcaa tacaattctt acgcctgtag
      121 gattagtaag aagacttata gtgccaactt gaaactataa atcatcggta caatccctga
      181 ttttattgtt gacatttcat ttatgccgac tatttatatg gtatacttgt cgaattatct
      241 taaaggaagc tcagattttc ttatttttat tgagaaaatg agatgacgcc ttatgtctgt
      301 attactacag ggagaaggga gatgcttcat tgcaaaggga ataatctatg aacgcaataa
      361 ttattgatga ccatcctctt gctatcgcag caattcgtaa tttattgatc aaaaacgata
      421 ttgaaatctt agcggagttg actgaagggg gaagcgccgt tcagcgggtg gaaacactta
      481 agcctgatat cgtcatcatt gatgtcgata tccccggagt taacggtatc caggtgttag
      541 aaacgctgag gaagcgccaa tatagcggaa ttattattat tgtctccgct aaaaatgacc
      601 atttttacgg gaaacattgt gctgatgctg gcgctaatgg attcgtgagt aaaaaagaag
      661 gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa tggctactgc tatttcccct
      721 tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca gcaaaaactc gactccttat
      781 cgaaacaaga aattagtgtc atgcggtata ttcttgatgg caaggataat aatgacattg
      841 ctgaaaaaat gttcatcagc aacaaaactg tcagcactta taaaagtcgc ctgatggaaa
      901 aattagaatg taaatcactg atggatcttt acacattcgc gcaacgtaac aaaatcggct
      961 aatcacatga agtttttacc ctattttttt cttctctgtt gtggtctttg gtcgaccata
     1021 agtttcgcag acgaagatta catcgaatat cgtggcatca gtagtaacaa ccgtgtcaca
     1081 cttgatccag tacgtctgag caacaaggaa ttacgttggt tagcgagcaa aaaaaatctt
     1141 gtgattgcag tacacaagtc ccaaacggct accttgttgc ataccgattc gcagcaacag
     1201 gttcgtggta ttaatgctga ttatttaaat cttttaaaaa gagcgttaaa tatcaaatta
     1261 acactccggg aatacgcaga tcatcaaaaa gcaatggacg cgctggaaga aggtgaagtc
     1321 gatatagtgt tatcacattt agttacttcg ccgcctctta ataatgacat tgctgcaacc
     1381 aaaccattga taattacctt tccggcgctg gtaaccaccc ttcacgactc aatgcgaccg
     1441 cttacctcac caaaaccagt aaatattgct cgggtagcaa attacccccc agacgaggta
     1501 attcatcaat catttccaaa agcaacaatt atctctttta caaatttata tcaggcatta
     1561 gcatccgtct cagctgggca caatgattac tttattggta gtaacatcat taccagcagt
     1621 atgatttccc gct
//
LOCUS       DAZFRN010000356         1627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFRN010000356 DAZFRN010000000
VERSION     DAZFRN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.359"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            170..>1627
                     /locus_tag="ACHKUB_005219"
     CDS             170..>1627
                     /locus_tag="ACHKUB_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolygalacturonate lyase"
                     /protein_id="HFO6658080.1"
                     /translation="MNKHTLLLTVLFLNLICTPVFAQNWQVATFGQSTDLNFSSLIDS
                     AKIGRNNAWLAGNNNFLEAGKFYTLPTDFFIESRGGKIANSHDGMTVFYTIVPVTQTF
                     RLEADLTLEQIGPEVNGKSPAGQEGAGLFVRDIIGPQRQEPQSAGTEEYPQASNILMN
                     AFITQNKKNDNLVQITSIVREGVIKTWGNEGITIKKQPIIENINFTQKRNIHMTIERL
                     PEKFILTAFDTDRKENQSWQFSDYSGFMNQLDNNSLAIGFFAARNAKLRVKNASFKPG
                     KPLVDYKQLTSRQFSRIRHKAPELFLASPQSVVRNSTTLQFLANQAGIVSIDNDKQTK
                     QVQAGELVQFPVTLQKKHNDFTVNFNVDGNISKKAIRIEQVKSNLTDPYEIYVCSDCR
                     QGARGSKNDPVDLQTAVKFVAPGGNIYLNDGQYHGITLDRELSGIPGKYKTISAINPH
                     KAIFINKTFNLDASYWHLKSVVFDGNVDNGNNKPAY"
BASE COUNT          540 a          299 c          339 g          449 t
ORIGIN      
        1 gccgaaataa ctaaagaatt agagcgtctg aaaaaaggtg gcagaaaaga agatgctaca
       61 ccggaaattc gtaagcttgt taagcaactg actggttatg aatatgacca gtgcttcggt
      121 aataacaatg ttggctttaa atttgaaaac aaagcagggg cattctagaa tgaataagca
      181 cacactatta ctgactgttc tttttctgaa tttgatttgt actcccgttt ttgctcaaaa
      241 ctggcaggtg gcgacgtttg gtcagtctac ggatctcaac ttttcatcgc tgatagattc
      301 ggccaagatc ggacggaata atgcctggct tgcaggaaac aataattttc ttgaagctgg
      361 aaaattttac actttaccaa cagatttttt tattgaaagc cgtgggggaa aaattgctaa
      421 ctcccatgac ggtatgaccg tcttttatac tattgttccg gttactcaga cattccgact
      481 ggaggctgat ttgacattag aacagattgg tccggaggtg aatggaaaat caccagcggg
      541 acaggaggga gctggattgt ttgtcagaga tattatcggt cctcagcgac aggaacctca
      601 gtcagctgga acagaagaat atccccaggc ctctaatata ttgatgaatg cctttattac
      661 acagaataaa aagaatgata acttagtaca gattacttca attgttcgtg aaggagtaat
      721 aaaaacatgg ggtaatgaag gtattacaat taagaaacag ccgatcattg agaatataaa
      781 ctttacgcaa aaaagaaata ttcatatgac gatcgagcga ctaccagaga agttcatcct
      841 gaccgctttt gataccgatc gtaaagaaaa tcagtcatgg caattttctg attactcagg
      901 ctttatgaat caactggata ataatagttt agctattggt ttttttgccg cacgaaatgc
      961 gaaactaagg gtgaaaaatg catcatttaa accgggcaag ccactggttg attacaaaca
     1021 attaacttca cgtcaattca gtcgtatccg gcataaagcc cctgaacttt ttcttgcttc
     1081 acctcaatcc gttgtaagaa actcaacaac tcttcaattt ttggccaatc aggctggaat
     1141 agtcagtatt gataatgata agcagactaa gcaggtgcag gcgggtgaac tggtacagtt
     1201 tccagttact ttgcaaaaaa aacataatga cttcaccgtc aactttaacg ttgatgggaa
     1261 tatatcaaaa aaagctatac gcatagagca ggttaaatca aacctgactg atccttatga
     1321 gatttacgta tgtagtgatt gtcgacaggg ggccagaggc agcaaaaatg accctgtaga
     1381 tttacagaca gccgtaaaat ttgtcgcacc cggcggtaat atatacctta acgatggtca
     1441 atatcatgga attaccttag atcgggaatt aagtggaata cctggcaagt ataaaacaat
     1501 ttctgccatt aatccacata aagccatttt tataaacaag acattcaatc tggatgcaag
     1561 ttactggcat ctaaaatccg tggtctttga cggcaatgtg gataatggaa ataataaacc
     1621 agcatat
//
LOCUS       DAZFRN010000357         1573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFRN010000357 DAZFRN010000000
VERSION     DAZFRN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.360"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..269)
                     /locus_tag="ACHKUB_005220"
     CDS             complement(<1..269)
                     /locus_tag="ACHKUB_005220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658081.1"
                     /translation="MWWLVTAPVRELARNALLMAVWRVSLHKTLLYYVYQRVTRLEQP
                     VQKCPRSEHGKFSPYNGFLTPLLFSFSHINDGVYSDGRVFRLCQSS"
     gene            complement(470..892)
                     /gene="subB"
                     /locus_tag="ACHKUB_005221"
     CDS             complement(470..892)
                     /gene="subB"
                     /locus_tag="ACHKUB_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO6658082.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSSVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(909..>1573)
                     /gene="subA"
                     /locus_tag="ACHKUB_005222"
     CDS             complement(909..>1573)
                     /gene="subA"
                     /locus_tag="ACHKUB_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO6658083.1"
                     /translation="RATESIMSNVFLAPGEEKIINISGGQKGISSASVWSELLSRMGR
                     NNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSVNKKQDPVIRVAALAQYRKGET
                     PVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATAIVSGVLAAMVS
                     CNPRATATELKRTLLESADKYPSLADKVTEGRDLNAEKAISMFCKKNYIPVRQGRMSE
                     EL"
BASE COUNT          415 a          405 c          329 g          424 t
ORIGIN      
        1 gaagactggc acagacgaaa tactcttccg tctgagtaaa caccatcatt tatatgagaa
       61 aaagaaaaca aaagaggggt gagaaaccca ttgtatgggg aaaacttgcc atgctcactg
      121 cgggggcatt tttgtactgg ctgttctaac cgggtaaccc gttgatacac atagtacaat
      181 aatgttttat gcaggcttac gcgccagact gccatcagta gtgcattgcg tgccagctcg
      241 cggacagggg cagtgaccag ccaccacatg agtaagcatc atttcagcgc catcaggcag
      301 ctcctgaaac tcctgcgagt tgaaaaagca tcctcctgca ttagcaatac tggcttgtca
      361 gtgccagtgt gatataagac gtaatgtcaa agttcgtggt gttgacatat ttctgtcgca
      421 accaaagtat gctcccgccc agtatatatc tgggcaggag catcatttat tagttctttt
      481 tcctgtcggg gccaaaacat tctgtcgatg tggcgcaggt tgataaccca acaagggcat
      541 tggacgttaa tgccttcaca aagggcggat acgtccatac cccgggttca taataaatcc
      601 tgaccaactg acctgttgtg taaaaatata gtgcctgatt atataatgtg gaaaacgaag
      661 gcccccagac agatgaattc ttcatcgagc aggctcttat ggaagaacca ccaggttgtt
      721 tcccttcaat acaaaaataa gggttattat ctatttgccc tgtatgaaac tgatcaataa
      781 caacgcttga aaacatgcca tcccgggcat cgccagtcca ctcagccatg gcaggattaa
      841 gggataaaca tcccataaca cctgcacata caaaaaacct cttaatcgtc atattcacct
      901 ccggaatttt acagttcttc actcatcctt ccctgacgga caggaatata atttttcttg
      961 cagaacatgc taattgcttt ttctgcatta agatctctcc cttccgtcac cttatctgca
     1021 agtgaggggt atttgtctgc agactccagc agtgtacgct tcagttctgt cgctgttgca
     1081 cggggattac atgagaccat cgctgccaga acgccggaaa ctatagctgt tgcttccgag
     1141 gttccactac cgatccccgt ttttccatct ggtctgagga atgtaatatt ctgtcccggt
     1201 gcggcaatat caacccagcc gtttccgaac cgacttccgg tgacccctcc accatgcaat
     1261 acaggtgttt ctcctttccg gtactgtgcc agagctgcca ctcttatcac aggatcctgc
     1321 tttttattca cactgctgac cggatggtaa gccgcaggcc atattctctg ctgagcactc
     1381 agttttcgta tatctgcgcc atcattaccc actgccgcaa caattaatcg ctcattattc
     1441 cggcccatac gggaaagcag ttcggaccag accgatgcgg aagatatccc cttctggcct
     1501 cccgatatat taatgatttt ctcttctcct ggtgcaagaa aaacatttga cataatgctt
     1561 tcagttgctc taa
//
LOCUS       DAZFRN010000358         1572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFRN010000358 DAZFRN010000000
VERSION     DAZFRN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.361"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(17..189)
                     /locus_tag="ACHKUB_005223"
                     /pseudo
     CDS             complement(17..189)
                     /locus_tag="ACHKUB_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
     gene            complement(224..>1572)
                     /locus_tag="ACHKUB_005224"
     CDS             complement(224..>1572)
                     /locus_tag="ACHKUB_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6658084.1"
                     /translation="AHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTT
                     DEAAEFTGMIPVYHFLVSAAIQVFMVIFWRTHRRGHRNWLALLLFVLCSINSWPLRMV
                     KGTVVGTTDTLREMQRYKQLNQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGY
                     PVPTTPWLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYS
                     TWWISNQGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSS
                     PKVIFLHMMGSHPNPCDRLHSWSNNYQERFPRKIACYLASISKLDNFLGQLDGILRRH
                     SRHFAMLYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARH
                     FAGIFQWLTGIRTENIPPFNPLTDEDNELIMVFNGDKNVPTDSLESQPLILPGKGK"
BASE COUNT          425 a          385 c          366 g          396 t
ORIGIN      
        1 aaattacttt aacgaattac tccatgaact cattaaagtt ctttcgtaat actttatcgt
       61 caaacacgtt atagctggca tcaaaaacca cagatttatc ctgccagcgg gtaatcacct
      121 ccccggagtt ttcggttggt ctggttactc ctggttatta gcaagataat tgctataaac
      181 agtggaaaac tcatcgtaca taacctggtg atgaacatta ctcttatttt cccttgcccg
      241 gaagaatcag aggctgtgac tcaaggctgt ctgtcggtac attcttgtct ccgttaaaaa
      301 ccataatgag ttcattatct tcgtctgtca gcggattgaa tggcggtata ttttcagtac
      361 gaatgccggt cagccactga aaaatacctg cgaaatgacg ggcactgatt tttctgctga
      421 cagactgatg agacgtgatg tcactggcgg taataatcag gggaacgctg tagcctccct
      481 gcacatgacc atcatgatga acaggattag cactgtcgct gaccgacaga ccatggtcag
      541 aaaagtaaag catggcaaaa tgacgggaat gccggcgaag gataccatca agctgcccga
      601 gaaagttatc cagtttactg atgctggcga ggtaacaggc aatcttccgg gggaaccgct
      661 cctggtaatt attcgaccag gagtgaagcc ggtcacacgg gttcggatga gaccccatca
      721 tgtgcaggaa tatcaccttc ggagaggatt tatccgccag tgcacgttct gtttcctgta
      781 acaacaacat gtcatccgtt ttacgggaag caaagctgcc tttcttgagg aaaacggtat
      841 gctccgcatc agaagcaata acagagatgc gtgtatcatg ctcccccagc tttccctgat
      901 tggatatcca ccatgtgctg tatcctgctt ttgctgccag cgccaccacg ttgttgccgg
      961 agtcagggtt ctgctcatag tcataaatca gtgtccggct cagggaaggt acagtactgg
     1021 ctgctgccga tgtatagccg tcaataaata aaccgggagc tgtattcagc cacggcgtgg
     1081 ttggcacggg atagccatat accgacatat aatccctgcg cacactctca ccagtgacga
     1141 taacaatcgt gtcatacaac ggtacacccg gcaggatttt ccagttgtca gccccgtgct
     1201 gattcagttg tttataacgc tgcatttcac gcaatgtgtc agttgtcccc acaacagttc
     1261 ctttaaccat ccgcaacggc cagctgttta ttgagcataa tacgaacagc agcagtgcca
     1321 gccagttacg gtgaccacgg cggtgtgttc gccagaaaat caccatgaat acctgaatcg
     1381 cggcactgac cagaaaatga taaacaggaa tcatcccggt aaactccgct gcctcatcag
     1441 ttgtggtctg caacaacgcg acaataaaac tgttgttgat tttaccgtac gtcataccgg
     1501 caggcgcata cagtgcacaa cagaacagaa ataacagcgc tgtaatggat gtgagggtat
     1561 ttctgtgtgc aa
//
LOCUS       DAZFRN010000359         1572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFRN010000359 DAZFRN010000000
VERSION     DAZFRN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.362"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..746)
                     /gene="tssI"
                     /locus_tag="ACHKUB_005225"
     CDS             complement(<1..746)
                     /gene="tssI"
                     /locus_tag="ACHKUB_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO6658085.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQ"
BASE COUNT          445 a          373 c          351 g          403 t
ORIGIN      
        1 tcctggtact ggccttcctg atcaaaacgc cctgcccagc cgggtcgttt aaaggtgtag
       61 tccttggtca ccacggaaga agggcggatt tgtgcactgt agcggaactg gctgatgcag
      121 agggtgctca cctcggtacg ggtgttcgga ttccagggga tctcaaagga ctccggcaga
      181 taacgcacgg tgtcgcacag gaccaggctc tggtcggtac ttttttgcgc gtgctcctca
      241 taaaagaaga tgccttcctc cgccgccatc cggcacagga aatcgtaatc ggtttcgccg
      301 tactggacac aaaactcacg ggaaggatgc ggctcgctga acagcggact ccactcggtc
      361 acaccgtttt ccttcaggat cgtagcgagg atgctttcga tgtcctcatt ctggaagata
      421 cggaagttct ggcgcagccc tgtgcgccac agcggcgggc acaccttcat gctgtacagc
      481 atctggtttt tgtcgttctc ccccagttca aaccaggtca ccacaccttt cacccggcgc
      541 tgtacgtcat cgccctgcca taccgtcagg taggccattt tgtccagcac ctgcgcaaat
      601 tcaagggaaa gaaactgctg actgaccaga gaaaggtcga gggaaaaaag tgaagagagt
      661 gactgggtga gatgaaagga aaccaccgca aacgcgtccg gtggcaggcc gtccacttca
      721 agtgtgaaac gtaatccggt tgacatagca acctccgtga aaatgaataa taatccgcac
      781 atattgcatg tacaacatgt cgggcggatg agataagcga gtcgacatct ggcgttaccg
      841 gggaatatca gaaaaatgtc atggttatgt gatgatttag tctgggattt tacttcagag
      901 aaacgggtgt ctgtttagtc aacaaaataa tcacaaacgc aacggatggg ttctcgacgt
      961 tattcacgac aggctcgtac attacagcga gtaataaaaa acctgatatg caaagtcaaa
     1021 ccaatattca taatgtcttt ttattcacaa caataaataa ttcaataaca atcattgaaa
     1081 tgaatggtga tttgtattag gaatatacgc taccgcccca ccctgataac atgatctgca
     1141 cattgccatc ttaacgtcgt taaacactgg cttaatagtg gatatttcaa attaggaatt
     1201 tattaaattc acctgatgaa tatcataagc aaatagcata gaaaataatg acaattcccc
     1261 cgaggggacc atttccaaag cactgcacca ccaccaggtg cccggatgat cttgcttcag
     1321 tggtaagagt acctgattca ttgttcccaa aatgatgttt tccccggccc cgccaataat
     1381 gtgcagctct cacgtgataa tctattgatt aggttatatt aagaatcaat atttaaaata
     1441 catccatttt cttgaataaa tcaacatgtt tatttatcaa ttgctgtttt agctctgact
     1501 catcaagctc tccatatttg tcctacagat aattgcgaat aaatacagag aacgccattt
     1561 atccttttag ca
//
LOCUS       DAZFRN010000360         1570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFRN010000360 DAZFRN010000000
VERSION     DAZFRN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.363"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(183..1376)
                     /gene="tyrB"
                     /locus_tag="ACHKUB_005226"
     CDS             complement(183..1376)
                     /gene="tyrB"
                     /locus_tag="ACHKUB_005226"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO6658086.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT          426 a          404 c          378 g          362 t
ORIGIN      
        1 ccaaaaaaac aggataagga attccagata attaggataa gcttaacaat aaaagaacta
       61 ataacgttta ttatctctca cgtagaacga tggcatcaaa aaaatcatct ataaagaaag
      121 agtgaaagcg acgacagttt aatttcactg caggctgggt agctccagcc tgctttcctg
      181 cattacatca ccgcagcaaa cgcctttgcc acacgttgta catttgccgt atttaacccg
      241 gcgacacaca tgcgaccgct ggcgatgaga tagacaccaa attcttcacg tagtcggtca
      301 acctgagcgg cacttaaacc ggtataactg aacatgccgc gctgattaag cagataatcg
      361 aaattgcgtt ctggcatctc tgtgcttaat accttcacca attcctgacg cattgccaga
      421 atgcgagtac gcatctcttc tacttccgcc agccagctgg ctttcaatgc ctcgtcattc
      481 agcactgcag ccaccacctg cgcaccaaaa ttcggcgggc tggagtagtt gcggcgaact
      541 gttgctttca attgccccag tacgcggcca gcggcttcgg catcttcaca cagaacagaa
      601 agtccgccga cgcgctcgcc gtaaagggag aaaattttcg agaacgaatt gctcaccaga
      661 gcgggtaatc cagcgctggc aatggcgcga atagcgtagg catcctcttc cataccggca
      721 ccaaatcctt gataggcaat atcgaggaat ggaataagct cgcgggcttt gagaatttca
      781 atcaccgcat cccactgatc attagtgaga tcggcacccg ttgggttgtg gcaacatgga
      841 tgcagcaaca caatactgcg ggcaggtaat gttttcagcg tcgccaacag gtcattaaag
      901 cgcacgccgt tagtcgcttc gtcataccag gggtaagtac tcacttcgaa tccagccccg
      961 gcgaatattg ctacgtggtt ttcccaggta ggatcgctga cccagacgcc tgattccggg
     1021 aagtagcgtt tcaggaaatc cgcgcccact ttcaatgccc cggagccgcc aagggtttga
     1081 atggttgcta cgcgctgttg tttcagtacc ggatggtccg caccaaacag cagcggcgca
     1141 atggcatggc gatagctgtt aagcccttcc atcggtaaat aaagcgaagc gccatgaggc
     1201 tgcgcattca ggcgcgcttc cgcctccgcc acggctttca gttgtggaat aattccgtct
     1261 tcgttgtagt acagaccgat acttaaattc actttgtcgc tgcgagggtc ttctttaaaa
     1321 cgctccataa gcgtaagaat cgggtcgcca gcgtaggcgt caactttttg aaacacgcga
     1381 tggttctcca ggtttacggg caggtggtta aaacacaata aaccggaaga aggcgaagat
     1441 cgagtggatg ttcaggagcg aacggcaatt agcaacagag tgagactcat gacaaacgta
     1501 catccgccag aggcacgacc tttataagag catgaaagaa catcaactta ttgaattttt
     1561 aggattttat
//
LOCUS       DAZFRN010000361         1556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFRN010000361 DAZFRN010000000
VERSION     DAZFRN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.364"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            1..527
                     /locus_tag="ACHKUB_005227"
                     /pseudo
     CDS             1..527
                     /locus_tag="ACHKUB_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
     gene            569..1024
                     /locus_tag="ACHKUB_005228"
                     /pseudo
     CDS             569..1024
                     /locus_tag="ACHKUB_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578540.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1153..1512
                     /locus_tag="ACHKUB_005229"
     CDS             1153..1512
                     /locus_tag="ACHKUB_005229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658087.1"
                     /translation="MTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKEL
                     HTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSG
                     HPISNMQGTPEKPSDIR"
BASE COUNT          475 a          298 c          372 g          411 t
ORIGIN      
        1 tgcgttcgag ctacggcaat cgagtcaacg gctcagcgat gcaagaaaaa ccaaatttat
       61 tcacctaaaa tagtagaaca atctcaaatt actgcttttg ataaatcgac taatgtcaga
      121 cgtgctacag catttgctat ttctgttatc aatgataaag caacaattcc actattgatt
      181 aatctgttaa aagatccaaa tggagatgtc agaaactggg ccgcatttgc aataaatatc
      241 aataaatatg ataatagtga tattagggat tgttttgtgg agatgcttca ggataaaaat
      301 gaggaagtcc gtattgaagc aataatcgga ctttcctaca gaaaagataa aagggtttta
      361 tctgttttat gcgatgagtt aaaaaaaata ctgtttatga tgatatcatt gaagctgcgg
      421 gtgaattagg tgataaaacg ctacttcctg ttttagatac tatgttgtac aagtttgatg
      481 acaatgaaat tataacttcc gctattgata agctgaagcg ttcatagcgt gagttgccta
      541 tgcacagtgg gggattcccg tcggcacggt gcagatgctc gaccggctgg aaagtgaaat
      601 cctggctgac cgggtgagtg aggaaagccg ccgctggctg gcatcgtgcg gcctgaccgt
      661 ggagcagatg aaaaaccaga tggacccggt gtacacgccg gcgcgaaaaa tccacctgta
      721 ccactgcgac catcgcggcc tgccgctggc gctcatcagc acggaagggg caacagcgtg
      781 gtgcgcagaa tatgatgaat ggggcaacct gctgagtgat gagaacccgc atcatctgca
      841 gcagctcatt cgcctgccgg gtcagcagta tgatgaggag tccggcctgt attacaaccg
      901 ccaccgctat tatgacccgc tgcaggggcg gtatatcact caggatccga ttgggctgaa
      961 ggggggatgg aatttttatc agtatccgtt gaatccggtc ataaatgtag atccgcaagg
     1021 tttggttgat ataaatttat accccgaaag tgatcttatc cattctgtag ctgatgagat
     1081 taatatccca ggcgttttca caatcggggg catggtaccc ccacatctat tgaatccgca
     1141 acgcgcagta tcatgacagc taaagatcta gcatatctaa ttaaatttga tgggaattat
     1201 aaagatggga tgacagtttg gttattttct tgtaatacag gtaaaggaca aaattcattt
     1261 gctagccaat tagctaaaga gttacataca aatgtaatag gacctgacac gctatggacg
     1321 tggtgggggc gaggaactaa tggtaagtta aaaatggata cagtgctaac agcaccaacg
     1381 aaccttaatt caaataagga tctaatggct ataacaacaa aagaccttgg taattggata
     1441 acatatgggc catctgggca ccccatttct aatatgcaag gtacgccaga aaaacccagt
     1501 gatataagat aggttgtaga tgtatgaaag catgcttgtt actatttttt tatttt
//
LOCUS       DAZFRN010000362         1521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFRN010000362 DAZFRN010000000
VERSION     DAZFRN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.366"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..125
                     /locus_tag="ACHKUB_005230"
     CDS             <1..125
                     /locus_tag="ACHKUB_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6658088.1"
                     /translation="VKNANRIIVMDNGFISEDGTHKELISKKDSLYAYLYQLQA"
     gene            129..>1521
                     /gene="ehxD"
                     /locus_tag="ACHKUB_005231"
     CDS             129..>1521
                     /gene="ehxD"
                     /locus_tag="ACHKUB_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO6658089.1"
                     /translation="MRFYMKGLWDLICRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVIWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGAFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNARGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYL"
BASE COUNT          535 a          226 c          320 g          440 t
ORIGIN      
        1 ctgtaaaaaa tgcaaacaga ataattgtta tggataatgg atttatttct gaagatggca
       61 cacataaaga gcttatctcc aaaaaagaca gtttatatgc atatttatat cagttgcagg
      121 cataacgtat gcgcttttac atgaaaggtt tatgggattt gatttgccgt tataagacgg
      181 ttttttctga tgtctggaaa attcgtcaca cacttgatgc tcctgtgaga gaaaaagatg
      241 agtatgcatt tcttcctgct caccttgaac tcattgaaac accagtatcc agacgttctc
      301 attttgttat atggagtatt ttattatttg taattatatc tcttctctta tctgttcttg
      361 ggaaagttga agtggtctca gtagcaaatg gtaagtttac tcatagtgga agaagcaaag
      421 aaataaaacc gatagagaat gcgattgtag aaaaaataat ggtgaaagat ggcgcttttg
      481 taaaaaaaaa tgatccatta gttgaattga cggtgcctgg tgttgaatct gatattttaa
      541 aatcagaagc atctttgttg tatgaaaaaa cagaacaata tcgctacgca atcctttctg
      601 aatcaataca acggaatgag cttcctgaaa taaggataac agattttcct ggcggagaag
      661 ataatgcaag aggtgaacat tttcagaggg ttagctcatt aataaaagag caattcatga
      721 catggcagaa cagaaaaaat caaaagcagc tcacattaaa taaaaaaata gttgaacggg
      781 atgcagcgct tgctcgtgtt agcctgtatg agcatcaggt atcacaggaa ggaagaaaac
      841 ttaatgattt taagtatttg ttgaataaaa aagctgtttc tcaacattca gttatggagc
      901 aggaaaatag ctatattcag gcaaaaaatg aacatgcagt ctggcttgca caggtttctc
      961 aacttgaaaa agaaatagaa cttgtgcggg aagaactggc actggagacg aatatcttca
     1021 gaagtgaaat tatcgagaag cacagaaaat caacagataa cattgtgtta ctggagcatg
     1081 agcttgaaaa aaacaggcag agaaaagcat catcttttat taaagctcct gtgagtggta
     1141 ctgttcagga gttaaatata catacagaag gtggtgttgt cacaacagca gaaacgctga
     1201 tgattattgt ccctgataat gatattctcg aagtaacagc atctgtactt aacaaggata
     1261 tcggttttat acaacctgga caggaggtcg ttattaaagt agatgcatat ccatatacac
     1321 gtcatggtta ccttacaggg aaagtaaaaa atataactgc agattctgtt tctgttccgg
     1381 atacaggact tgtatttaac gtgattatat ctgttgatcg gaatgatata cagggagaaa
     1441 gaaaaaaaat tcctgttaca gctggaatga ctgttatggc agaaataaaa acaggagtgc
     1501 gtagtgttat cagctatctt c
//
LOCUS       DAZFRN010000363         1500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFRN010000363 DAZFRN010000000
VERSION     DAZFRN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.367"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            180..995
                     /gene="cma"
                     /locus_tag="ACHKUB_005232"
     CDS             180..995
                     /gene="cma"
                     /locus_tag="ACHKUB_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HFO6658090.1"
                     /translation="METLTVHAPSPSTNLPSYGNGAFSLSAPHVPGAGPLLVQVVYSF
                     FQSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAY
                     HFAKPAPSQYDYRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKI
                     NQIKDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYN
                     GVVRSYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(1045..1398)
                     /gene="cmi"
                     /locus_tag="ACHKUB_005233"
     CDS             complement(1045..1398)
                     /gene="cmi"
                     /locus_tag="ACHKUB_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO6658091.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPVCYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
BASE COUNT          434 a          327 c          273 g          466 t
ORIGIN      
        1 tttaatggag tgtacagata tatatcttat atatggtagt tcaggtgctt ccctaccatt
       61 tttaacggca tatccggcaa acaatgttat ggggagcctc tctcatgggg ttcgttcgcc
      121 ggtgtaagcg ttagccagcc gcgaactctg ttatcttatt aacttataag gagttatgta
      181 tggaaacctt aactgttcat gcaccatcac catcaactaa cttaccaagt tatggcaatg
      241 gtgcattttc tctttcagca ccacatgtgc ctggtgctgg ccctctttta gtccaggttg
      301 tttatagttt tttccagagt ccaaacatgt gtcttcaggc tttaactcaa cttgaggatt
      361 acatcaaaaa acatggggcc agcaaccctc tcacattgca gatcatatcg acaaatattg
      421 gttacttctg taacgccgac cgaaatctgg ttcttcaccc tggaataagc gtttatgacg
      481 cttaccactt cgcaaaacca gcgccaagtc aatatgacta tcgctcaatg aatatgaaac
      541 aaatgagcgg taatgtcact acaccaattg tggcgcttgc tcactattta tggggtaatg
      601 gcgctgaaag gagcgttaat atcgccaaca ttggtcttaa aatttcccct atgaaaatta
      661 atcagataaa agacattata aaatctggtg tagtaggtac attccctgtt tctacaaagt
      721 tcacacatgc cactggtgat tataatgtta ttaccggtgc atatcttggt aatatcacac
      781 tgaaaacaga aggtacttta actatctctg ccaatggctc ctggacttac aatggcgttg
      841 ttcgttcata tgatgataaa tacgatttta acgccagcac tcaccgtggc attatcggag
      901 agtcgctcac aaggctcggg gcgatgtttt ctggtaaaga gtaccagata ctgcttcctg
      961 gtgaaattca cattaaagaa agtggtaagc gataatataa gggccggggt tccggccttt
     1021 ttatgcagcc tttataacag tgtattagtc attcgcatat tcaacatatc cctcctcaca
     1081 atggtacatg ccataataca ttctcttacc ctccgggcct tcagctttaa atgggacaag
     1141 aagtgttttt tcatcttcaa catctgtcaa acttctctca atcttttccc atgtaatttt
     1201 cgggatttct gtaccaagaa gttgtacatc gttgtcccag cgtttcattc tttgcaggta
     1261 atcatttttt acaaacgttc tggcctgttc atcgctgacc tgatagcata ccggaccttt
     1321 atcttcagac cagaaaaaaa cagcagcaag agcaataatc gttaaaataa aaataaattt
     1381 catgctaatt actttcataa aatctccaca acttctgtcg ttcattctat ataaaaacac
     1441 attacgaata tatccgtaca atatgagcat actgtgaagt ggctttaaca attatgaaat
//
LOCUS       DAZFRN010000364         1489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFRN010000364 DAZFRN010000000
VERSION     DAZFRN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.368"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1489
                     /locus_tag="ACHKUB_005234"
     rRNA            <1..>1489
                     /locus_tag="ACHKUB_005234"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          410 a          316 c          469 g          294 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcacgcttag gcgtgtgact
      181 gcgtaccttt tgtataatgg gtcagcgact tatattctgt agcaaggtta accgaatagg
      241 ggagccgaag ggaaaccgag tcttaactgg gcgttaagtt gcagggtata gacccgaaac
      301 ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg
      361 actaatgttg aaaaattagc ggatgacttg tggctggggg tgaaaggcca atcaaaccgg
      421 gagatagctg gttctccccg aaagctattt aggtagcgcc tcgtgaattc atctccgggg
      481 gtagagcact gtttcggcaa gggggtcatc ccgacttacc aacccgatgc aaactgcgaa
      541 taccggagaa tgttatcacg ggagacacac ggcgggtgct aacgtccgtc gtgaagaggg
      601 aaacaaccca gaccgccagc taaggtccca aagtcatggt taagtgggaa acgatgtggg
      661 aaggcccaga cagccaggat gttggcttag aagcagccat catttaaaga aagcgtaata
      721 gctcactggt cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat gcaccgaagc
      781 tgcggcagcg acgcttatgc gttgttgggt aggggagcgt tctgtaagcc tgcgaaggtg
      841 tgctgtgagg catgctggag gtatcagaag tgcgaatgct gacataagta acgataaagc
      901 gggtgaaaag cccgctcgcc ggaagaccaa gggttcctgt ccaacgttaa tcggggcagg
      961 gtgagtcgac ccctaaggcg aggccgaaag gcgtagtcga tgggaaacag gttaatattc
     1021 ctgtacttgg tgttactgcg aaggggggac ggagaaggct atgttggccg ggcgacggtt
     1081 gtcccggttt aagcgtgtag gctggttttc caggcaaatc cggaaaatca aggctgaggc
     1141 gtgatgacga ggcactacgg tgctgaagca acaaatgccc tgcttccagg aaaagcctct
     1201 aagcatcagg taacatcaaa tcgtacccca aaccgacaca ggtggtcagg tagagaatac
     1261 caaggcgctt gagagaactc gggtgaagga actaggcaaa atggtgccgt aacttcggga
     1321 gaaggcacgc tgatatgtag gtgaagtgat ttactcatgg agctgaaatc agtcgaagat
     1381 accagctggc tgcaactgtt tattaaaaac acagcactgt gcaaacacga aagtggacgt
     1441 atacggtgtg acgcctgccc ggtgccggaa ggttaattga tggggttag
//
LOCUS       DAZFRN010000365         1437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFRN010000365 DAZFRN010000000
VERSION     DAZFRN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.369"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..102)
                     /locus_tag="ACHKUB_005235"
     CDS             complement(<1..102)
                     /locus_tag="ACHKUB_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6658092.1"
                     /translation="MTQTESVILAHARRCAPAESCGFVISTPEGERYQ"
     gene            complement(113..811)
                     /locus_tag="ACHKUB_005236"
     CDS             complement(113..811)
                     /locus_tag="ACHKUB_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6658093.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(811..1140)
                     /locus_tag="ACHKUB_005237"
     CDS             complement(811..1140)
                     /locus_tag="ACHKUB_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6658094.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1137..>1437)
                     /locus_tag="ACHKUB_005238"
     CDS             complement(1137..>1437)
                     /locus_tag="ACHKUB_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6658095.1"
                     /translation="EFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          297 a          524 c          320 g          296 t
ORIGIN      
        1 ctggtaccgt tcgccctccg gggtgcttat cacgaagccg cacgactccg caggcgcaca
       61 ccgccgggca tgcgccagaa tcactgattc agtctgtgtc ataaaacggg atttactgcg
      121 aaagtttatt gatggaaagg aaaccgccga aattagccac catgccgcgc atctcacacc
      181 cgcgcatgca tttactgcat ctgtccttcc ggatatccgt ggtagggttg tcgaactcat
      241 ccgccactgc cggaccgtta tacccgcact catcgccccg gtaatcccac atacaggtgt
      301 tcgccagcat gatgcgaccg ggaaacagcg ccccgtccgt ctccgtcggt gtcgccagca
      361 caaacgaagc cgtcatggcc gtcagcgatg acatctgctc caccacccac cggtccgtca
      421 gctcctgctc cgggtcggcc tcaggattgc ctgccacaaa gttcaccgca tccagaaaac
      481 gcgcatacac ccggcgacgg accaccgtgg cacccaccag gctctgcaaa tcctccgcca
      541 tcccggtgac cagcccgaaa agattggaca ctgtcagcga cgggcgggca ctgctgccct
      601 ttccgttcat ctcaaagccg ctgccctcaa tcgggtacgc ctgatattca cgcccctgcc
      661 aggtcactgg ctcccctttt tcattcagct cattgcagaa aaaataccgc tcaccgccct
      721 gcaccgtcag gtcgatttcc cagagcacca cccgcggtga ctgctctgat ttaaccgact
      781 cgttcaggct ttcttcgtga atatcctgca tcagttcacc acctgcttaa actccgcgct
      841 gaactcaacg cgcaacatcc cgacccgcgc agaccacccg gcacaggtca cctttatctg
      901 ccggtatgca tagggtggct tccacaaaaa tgccttccag cccccgtgct ctgccaggaa
      961 cgcttccaga tgccgggcct cctcccgggt cacggaaagc gtcacccggt atgttttcag
     1021 gtcagcattc agccctgccg ccatacgctg tgagtacccg tcaccaaaac gcacttcacg
     1081 caccgacggc tgcgagttca cctccatatc cggcttcact ttccagcgaa aggttttcat
     1141 cgcccgctcc ctgataacat accgccatca cgcaactgca gccggagttc atcctgcgcc
     1201 cccttacggg ccatgtcata caccgccttc atcagctgcg gccccgcctg tccgttgata
     1261 ccgtcgttct gaatcaccac gtgattgttc tgattaaaat taataccttc cgcccgccgc
     1321 atctgcgccg gacttccggc accacccaca taaccccctt ccgcatagcc gcgcatcagg
     1381 cggtaaagat tccccacacc tatccggctg gttgcctcct tcgtgaagac aaactcc
//
LOCUS       DAZFRN010000366         1430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFRN010000366 DAZFRN010000000
VERSION     DAZFRN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.370"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            487..714
                     /gene="ykgL"
                     /locus_tag="ACHKUB_005239"
     CDS             487..714
                     /gene="ykgL"
                     /locus_tag="ACHKUB_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO6658096.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(749..889)
                     /gene="ykgO"
                     /locus_tag="ACHKUB_005240"
     CDS             complement(749..889)
                     /gene="ykgO"
                     /locus_tag="ACHKUB_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO6658097.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(889..1152)
                     /gene="ykgM"
                     /locus_tag="ACHKUB_005241"
     CDS             complement(889..1152)
                     /gene="ykgM"
                     /locus_tag="ACHKUB_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO6658098.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
BASE COUNT          409 a          281 c          328 g          412 t
ORIGIN      
        1 tgcattttat attggtcagg aactttgccc tgaagtgtta ggaatagtct taatgcgctg
       61 tgtgatggac tagtaaaaaa acaagtttta agggtaaggg atagaatata aaactataat
      121 gagctgtcat tgaagatgat ttgccaatca gtaagaaatt ttaatggatt aacatgccgc
      181 acaaaatggt tataggtgtt cattacaata agtgatggaa aattgaacgg gcgtatgtac
      241 gtcatctgtt cggtaagccc ccaagagcca gattaagtga agttaaacga aggctgccag
      301 ctgtcacagg aacgatggcc tctttgtcag ggcactgagt gcagttatct gtgcgcgtta
      361 ctctaactgg cactcgaacg tcggcgacgg ggttaccggt ggatcagact gttcctgtgg
      421 cgtaaacgtt tttatgttag taccaacaga tttaccacat ttatcatctt aacagtttga
      481 gtgaaaatgc aggctgcgcc gcaaagggag gagactgagt ggcgtgttca gtctaaaaga
      541 gagttgatgc cagcatacag gggcgaagct ggtcaacaag tcaacattaa gataatggaa
      601 tattcggaga gaaacgtcag gcagttggcc tcaaatgaac aggaggaata tatccccagg
      661 aaaattaatg ttggtgttat aaacacacca acattaatcc gatctgatta ttaatcagac
      721 aaatatcgtt aatccgtcga attttgaatc aacgtttttt cttacgaccc tgaacggcct
      781 taaaacgtgg attagattta caaatcacat atagccgtcc ttttcgcttc acaatctgac
      841 agtctggatg gcgttctttt gcggtacgca gagagttaag aactttcatc acgacccctt
      901 tttcgtgcta acaaaacgac caaaacgttg ggtgaatcgt gcaacatttc cttctgatgc
      961 cactgttctc agcttccctg tatagaacgg gtgcgattta gaagagacat caattgtcac
     1021 gtatggatac gttacgccat ccagctcaat ctcacggtct gttttgatag tcgagccgat
     1081 tttaaagtac tcatcaacac tggtgtcgtg gaacaccaca gtacgatact caggatggat
     1141 attgggcttc atcattttta cctgttatgt tataacataa ccataaagat acatgctcat
     1201 ccggatcatt tcaacaccca atccctgtga ctctttgtgg atgtggaatt ttcgcaacgg
     1261 ggaactctgg cattgttagc agccattggt gacgctccca taaacgcctg gatgagtgat
     1321 tgatgatggt tttgctgaga gtatggattt ttcgacaatg gaggttatct ccactttact
     1381 ggccgatgat ttatcgcagt atgattgatt acctgacagc gtgtattcct
//
LOCUS       DAZFRN010000367         1413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFRN010000367 DAZFRN010000000
VERSION     DAZFRN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.371"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1413
                     /locus_tag="ACHKUB_005242"
     CDS             <1..>1413
                     /locus_tag="ACHKUB_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Contact-dependent inhibitor A"
                     /protein_id="HFO6658099.1"
                     /translation="APELTSSGVLVGSALNTQSQTLTNSGLLQGEASLTVNTQRLDNQ
                     QNGTLYSAADLTLDIPDIRNSGLITGDNGLMLNAVSLSNPGKIIADTLSVRATTLDGD
                     GLLQGAGALALAGDTLSQGSHGRWLTADDLSLRGKTLNTAGTTQGQNITVQADRWANS
                     GSVLATGNLTASATGQLTSTGDIMSQGDTTLKAATTDNRGSLLSAGTLSLDGNSLDNR
                     GTVQGNHVTIRQNSVTNSGTLTGIAALTLAARMASPQPALMNNGGSLLTSGDLTITAG
                     SITSSGHWQGKRVLITADSLANSGAIQAADSLTARLTGELVSTAGSKVTSNGEMALSA
                     LNLSNSGQWIAKNLTLKANSLTSAGDITGVDTLTLTVNQTLNNQANGKLLSAGVLTLK
                     ADSVTNDGQLQGNVTTITAGQLTNGGHLQGETLTLTASGGVNNRSGGVLMSRNALNVS
                     TATLSNQSTIQGGGGVSLNAT"
BASE COUNT          346 a          410 c          415 g          242 t
ORIGIN      
        1 tgcgccggaa ctgaccagca gcggcgtact tgttggttcc gccctgaata cacagtcaca
       61 gaccctgacc aacagcggtc tgttgcaggg ggaggcctca ctcaccgtta acacacagag
      121 gcttgataat cagcagaacg gcacgctgta cagtgctgca gacctgacgc tggatatacc
      181 ggacatccgc aacagcgggc ttatcaccgg tgataatggt ttaatgttaa atgctgtctc
      241 cctcagcaat ccgggaaaaa tcatcgctga cacgctgagc gtcagggcga ccacgctgga
      301 tggtgacggc ctgttgcagg gcgccggtgc actggcgctt gctggcgaca ccctctcaca
      361 gggtagtcac ggacgctggc tgacggcgga cgacctctcc ctccggggca aaacactgaa
      421 taccgcaggg accacgcagg gacagaatat caccgtgcag gcggacagat gggcgaacag
      481 tggttccgtg ctggcaaccg gtaaccttac tgcttcggca accggtcagt tgaccagtac
      541 cggcgatatc atgagccagg gggacaccac gctgaaagca gccaccacgg acaaccgggg
      601 cagtctgctt tcggccggca cgctctccct tgatggaaat tcactggata accgcggcac
      661 tgtccagggt aaccatgtca cgattcgcca gaacagtgtc accaacagtg gcacgctcac
      721 cgggatcgcc gcactgacgc ttgccgcccg tatggcatcc cctcaacctg cgctgatgaa
      781 taacggaggt tcattgctga ccagcggcga tctgacaatc accgcaggca gtattaccag
      841 ttccggacac tggcagggca aacgggtgct gatcaccgca gacagtctgg caaacagcgg
      901 ggcgatccag gcggctgaca gcctgactgc acgtctgacg ggtgagctcg tcagcacagc
      961 gggcagcaaa gtcacctcga acggtgaaat ggcgctcagt gcactgaatt taagcaacag
     1021 cggacaatgg attgcaaaaa atctgaccct gaaggcgaac tcactgacca gtgcgggtga
     1081 catcaccggt gtggatactc tcacgctcac ggtgaatcag acgctgaaca atcaggcgaa
     1141 cggaaaactg ctcagtgcag gtgtgctgac gctgaaggca gacagtgtca caaacgacgg
     1201 gcaattacag ggaaatgtca ccaccatcac ggcaggacaa ctcacaaacg gcgggcatct
     1261 gcagggcgaa acgctgacgc tgacagcctc cggtggcgtg aacaaccgtt ccggtggtgt
     1321 tctgatgagc cggaatgcac tgaatgtcag tactgcgacc ctgagtaacc agagcacgat
     1381 acagggtgga ggcggggttt ccctgaacgc cac
//
LOCUS       DAZFRN010000368         1369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFRN010000368 DAZFRN010000000
VERSION     DAZFRN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.372"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..903
                     /locus_tag="ACHKUB_005243"
     CDS             <1..903
                     /locus_tag="ACHKUB_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO6658100.1"
                     /translation="LKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGWED
                     IEDFGETHPDSTMHSLVLGQIKTDEKSNEITAIPELLNMMDIKGKIITTDAMGCQKDI
                     AEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLH
                     IVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFA
                     TAIRNHWHVENKLHWRLDVVMNEDDCRIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAGSGLS"
     gene            933..>1369
                     /locus_tag="ACHKUB_005244"
     CDS             933..>1369
                     /locus_tag="ACHKUB_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658101.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGFRNVQNGTTRNVVKDQQLLKQLRKV"
BASE COUNT          443 a          226 c          369 g          331 t
ORIGIN      
        1 cttaaaaaat tgatggaaca tatttctatt atccccgatt acagacaagc ctggaaagtg
       61 gaacataaat tatcggatat tctactgttg actatttgtg ccgttatttc tggtgcagaa
      121 ggctgggaag atatagagga ttttggggaa acacatcccg actcaacaat gcacagtctg
      181 gtcctcggac agatcaagac ggatgagaaa tccaatgaga ttacagccat tcctgaactt
      241 cttaacatga tggatattaa aggaaaaatc atcacaactg atgcgatggg ctgccagaaa
      301 gatattgcag aaaagataca aaaacaggga ggtgattatt tattcgcggt aaaaggaaac
      361 caggggcggc taaataaagc ctttgaggaa aaatttccgc tgaaagaatt aaataatcca
      421 gagcatgaca gttacgcaat gagtgaaaag agtcacggca gagaagaaat ccgtcttcat
      481 attgtttgcg atgtccctga tgaacttatt gatttcacgt ttgaatggaa agggctgaaa
      541 aaattatgcg tggcagtctc ctttcggtca ataatagcag aacaaaagaa agagcccgaa
      601 atgacggtca gatattatat cagttctgct gatttaaccg cagagaagtt cgccacagca
      661 atccgaaacc actggcacgt ggagaataag ctgcactggc gtctggacgt ggtaatgaat
      721 gaagacgact gcagaataag aagaggaaat gcagcggaat tattttcagg gatacggcac
      781 attgctatta atattttgac gaatgataag gtattcaagg cagggttaag acgtaagatg
      841 cgaaaagcag ccatggacag gaactatctg gcgtcagtcc ttgcggggag cgggctttcg
      901 taatcttgcc ctgggtagga ggccctccag ggatgttagt cggtgcggca gttggagcgg
      961 tggttattgg tggtggtata gtaatataca acaatcgcga ggctgttggg gatgcgattg
     1021 agaaaagtat ggagatgcgg ggtgaagccg aattattaaa tgcacaaagt caaatggaat
     1081 tctggcgaga tccctatggt gctttttcca gcgtgtttgg aaatgatgat tcatcaaaag
     1141 agaatccaaa tgtcgggaag gatttgacgg atgaagagaa gaactgtttg ggcagtggca
     1201 gtacaggagg ttcgggaggg tgggagccag atgaggatgg cgaaataaga aatacctaca
     1261 ggtctattaa ggatgctcct cagtatccca gagggtttag gaatgtccaa aatggcacta
     1321 caaggaatgt tgtaaaggat cagcagttat tgaaacaatt acgtaaagt
//
LOCUS       DAZFRN010000369         1369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFRN010000369 DAZFRN010000000
VERSION     DAZFRN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.373"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..348)
                     /locus_tag="ACHKUB_005245"
     CDS             complement(<1..348)
                     /locus_tag="ACHKUB_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO6658102.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYA"
     gene            complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHKUB_005246"
     CDS             complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHKUB_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO6658103.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYTAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          351 a          317 c          308 g          393 t
ORIGIN      
        1 ggcgtagtaa ctgcgtttgt tatgattaac atcagtaacc caccggaaaa cgcccgcgcc
       61 tgctagtgtt gaacagtatt cccgaaatgt agattttccg caaatatgaa gcaatgcggc
      121 ctcttttatt ttagcagggt tcttggtcgt actaactttt aacaggttcc tggttcctct
      181 taataacaaa accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata
      241 aatgtaatgt gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc
      301 ttttctcact tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac
      361 ctcttgttag ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc
      421 tgtcctttgg ctgtgatatg tacagagtgt gatagaggga atatctgaat tctcccggtg
      481 agcattttgc aacggaccag ctccggtaca aacgctgttg tgggttcaga ttataacatt
      541 ctgtctaagg ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt
      601 gctcacccca gtgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga
      661 caatcaatgt ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa
      721 cagaaatgtg ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct
      781 cttcaacatt ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa
      841 gcgaggctgc tgtgtattca attaatgcgc cgtcaaacat actgctcata atgcgggagg
      901 tgttgtgatc aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat
      961 ctactgggta ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca
     1021 tgcgaagaat tgacatcatc tcttcttgca ggcggctcca gtcatcttcc gtatcctggc
     1081 tggtggtttc cagtaatgct ttaactttgc ctacagggac gccattactt atccaacgct
     1141 tgatctcttc gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc
     1201 gctgtggttt taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc
     1261 cgcaacgttc agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcaca
     1321 aatctccgtc gcctgtacac gacccaataa tactttgtac aatatacgc
//
LOCUS       DAZFRN010000370         1356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFRN010000370 DAZFRN010000000
VERSION     DAZFRN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.374"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..628)
                     /locus_tag="ACHKUB_005247"
     CDS             complement(<1..628)
                     /locus_tag="ACHKUB_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6658104.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQK"
     gene            636..1061
                     /gene="tnpA"
                     /locus_tag="ACHKUB_005248"
                     /pseudo
     CDS             636..1061
                     /gene="tnpA"
                     /locus_tag="ACHKUB_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          362 a          338 c          297 g          359 t
ORIGIN      
        1 tcttctggtt tttctgaaaa ctgtttacag gctcaaagac tgtgccatct gacagcgtgg
       61 cgagtttagc cacgccagca tccagcccga ccattgatgc tgaagggtga accggagttg
      121 atacttcact ttctgtctga atactgatgt accacttacc gcaggactgg ctgacagtga
      181 catttttcac aacacccgtg acctgacggc tgttccggta gcgcatccag cccagtttcg
      241 gcagaaaaat acggctgttt tcctgatcga gcttaacgcc ctgcgggtag cggaatacat
      301 cattctgtcc ccgctttttg aatcggggaa aagcagcccg cttccggaag aagtttttgt
      361 aagcccgctc aaggtctttc agtgactgtt gcaatggctg tgacggggca tctttaagcc
      421 attgcgtttc agtggcattt ttccactcaa ccagccagga agccattttc ccgtaaggga
      481 tgtatttatt accgacctca tgattctcat tctgacgtgc cagagcacga ttgaaaacga
      541 aacgacatgc gcctgcgaag cgcctcattt cacgctcctg ttgaccaccg ggtcttaact
      601 ggaatttaaa tgcctgtaat cgtttcacag gccaaatgat actttggtct gtgaaaaaag
      661 aaaccgatat ccggcgtgga aggcattgtg ttttcctgat gcatgttcac ctggtctttg
      721 tcaccagata ccgacgtcag atttttgacc atgacgcgac agaaaaacta cgcacttact
      781 tttcaaatgt atgtgctgat tttgaagctg aactggttga aatggatggc gaaccagatc
      841 acgtccattt gttaattaac tatcctccca aactggcgat atccagtctg gtaaacagcc
      901 tcaaaggcgt atcgggtagg tgactgcgac gagatcgacc agatattgca gtcaggtatt
      961 actacaaagg cgttttgtgg agtgctggct attttgccag tagctgcgga ggtgcgccaa
     1021 tatccgtcat ccaccaatac attgaacaac agcaaacacc tggtcaggtg gaaaaccgcg
     1081 ccttatatcc ctgccctgaa ggacggggtt ttacggcgca ccggataagc ctggagatta
     1141 tctacaccag catggacatc attgtactat gccccgtaca tgccccatca catcctcaac
     1201 atcaaagtat tagagccttg caaaccatta tgcgtctcag tttgtctcat ctatcttacg
     1261 caaaaaaaat agaactaata tcaagcggcg ctatattgcg tattaaataa aaccacctca
     1321 attacattaa taaatccaaa caaacatcat gcaaat
//
LOCUS       DAZFRN010000371         1354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFRN010000371 DAZFRN010000000
VERSION     DAZFRN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.375"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..366)
                     /locus_tag="ACHKUB_005249"
     CDS             complement(<1..366)
                     /locus_tag="ACHKUB_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6658105.1"
                     /translation="MGVVLGIPFKRFSFNAWRFSHSGVGPFELQIGTFEFLSQNTSHT
                     EREHSHFDLKAKKYAGLMIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIF
                     GYIGITQSSNKELKDKSGRE"
     gene            302..1354
                     /locus_tag="ACHKUB_005250"
                     /pseudo
     CDS             302..1354
                     /locus_tag="ACHKUB_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
BASE COUNT          457 a          269 c          241 g          387 t
ORIGIN      
        1 ctctctcccc gacttatctt tcagctcttt attactggat tgagtaattc caatataacc
       61 aaaaatcctt ctattagacc aataatatcg ccctgcattc catgaacgtc tttcttctat
      121 ctggaagaaa tcattatcta ctcgaccata aggtaatact ctcaagctat ccctaaaaat
      181 cattagtcct gcatactttt tggccttaag atcaaagtgg gaatgttcac gttcagtatg
      241 tgatgtattt tgtgaaagga attcaaacgt tcctatctga agctcaaatg ggccgacccc
      301 tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaatgc ctaaaacaac
      361 acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa tgcgaaaata
      421 tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac aaatagaaag
      481 atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta acttatttaa
      541 cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa cttgtcagtt
      601 ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg ccattgattt
      661 ttcattgctc atgaaaacga taaaagcctt ttcatttggt ggagatcttc aaacccttgc
      721 atcaaaaccg ggttccacaa tctcaagtat cccatcagaa agacggatat tgattaacat
      781 taaccacgat tttcctaaca atggtaatct tttcaatgac tttctattta accatcaaca
      841 agatgaacaa ttagcaatgg catatatagc cgctctgccg ttcagcaggc ctttagttta
      901 ctgggatggc caagtattaa aatcaacgac tgaaattaaa aattatgatg ggtccacgcg
      961 tgtcggcggt gaggcgtagc ttaataaagg ttgctctacc tatcagcagc tctacaatga
     1021 attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg gtgtatctgc
     1081 aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc actctcctca
     1141 tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct ggcctaacaa
     1201 attgcaacct ggaatataca aaaacatggg gagtaatagc gttaacatta ttattaataa
     1261 tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa ctctaaatat
     1321 caatattcct gggcgtagcg ctcttctttt aggg
//
LOCUS       DAZFRN010000372         1353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFRN010000372 DAZFRN010000000
VERSION     DAZFRN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.376"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            59..>1353
                     /locus_tag="ACHKUB_005251"
     CDS             59..>1353
                     /locus_tag="ACHKUB_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6658106.1"
                     /translation="MTEENRRIKAITRFSYGSGNLIGSGALAISGAWLLYFYTTFCGL
                     TVFQATVIFSIATYLDVILNPLMGFITDNFYQTKLGRRFGRRRFFILIAIPCMIIYPL
                     LWVNGMSFWYYLSTYIIFEVVYTMIMIPYNTLPVEMTKDFNQRTYLTGSKAMFGKVAN
                     FLAAAIPGIYFYFFNKDSATPFFLTGLTYAIIMITSLIMLYFNSWERPTDDVVDESTH
                     GFMEGFNKLFTDLFSTFKLKTFRTHLGMYLFGFGAEWLFTATFTYFIVFNLEQPRTFV
                     SEMNSLSSICQFFSTAFFMMWCAKRGFKRPFIIAILIVVSSVIGYVGVYYLNIPHLTW
                     LIIGITIWFGLGTGGVYYIPWSVYVFLADVDEVLTGRRREGLYAGAMVMAGKLMRATV
                     VFCLGVVLSLFGFESGIHHQSASAVNAINGVMIFGVAGMA"
BASE COUNT          337 a          238 c          279 g          499 t
ORIGIN      
        1 atactccatc ttttccagga gatggaaatg gacttttgtt gtggaagagg tatcataaat
       61 gactgaagaa aacagaagaa taaaagccat aactcgtttt tcatatggta gtggaaatct
      121 tatcggcagt ggagctctgg ctattagtgg agcatggtta ctttatttct acaccacatt
      181 ttgtggattg acagtttttc aggccactgt aatattttcc attgctactt atctggatgt
      241 catcctgaat ccactaatgg ggtttattac ggataatttt tatcagacaa aactcggtcg
      301 tcgctttggt cgtcgtcgct tcttcatatt aattgccatt ccatgtatga ttatctatcc
      361 attgctttgg gtaaatggta tgagtttttg gtattatctt tctacttata tcatttttga
      421 agtggtatat actatgatta tgataccgta taatacctta cctgttgaaa tgacaaagga
      481 tttcaatcaa aggacctatt tgacaggttc taaagctatg tttggtaaag tagctaattt
      541 cttagcagcc gctattcctg gtatctattt ttacttcttc aataaagatt cagccacccc
      601 atttttcctg accggtttaa cttatgctat tattatgatc acttcattaa taatgctcta
      661 tttcaacagt tgggaacgtc caactgatga tgtagttgat gaatcaacgc atggttttat
      721 ggagggcttt aataaacttt ttaccgatct tttctcaacc tttaagctaa aaaccttcag
      781 aacccatctt ggtatgtatc tatttggttt cggcgctgaa tggctattta cagcgacatt
      841 tacctatttc attgttttca accttgaaca accgagaaca tttgtttctg agatgaactc
      901 attaagcagt atttgtcagt tcttctctac agcattcttt atgatgtggt gtgccaaacg
      961 aggatttaaa cgcccattta tcattgctat cttaattgtt gtatcttcag tgattggtta
     1021 tgttggtgtg tactatctta atattcccca tctgacctgg ctcattattg gcatcactat
     1081 ctggtttggt ctggggacgg ggggggtata ttacattccg tggagtgtat atgtattcct
     1141 tgccgatgtt gatgaggtct taactggtcg tcgtcgtgaa ggtctttatg ctggtgcaat
     1201 ggttatggcc ggtaagttaa tgagagccac tgtagtattt tgtctggggg ttgtcctcag
     1261 tctgtttggt tttgagtcag gcattcatca tcaatcagcc agtgcagtaa atgcaattaa
     1321 tggcgtcatg atatttggtg tggctggtat ggc
//
LOCUS       DAZFRN010000373         1329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFRN010000373 DAZFRN010000000
VERSION     DAZFRN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.377"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(1..508)
                     /gene="ybfC"
                     /locus_tag="ACHKUB_005252"
                     /pseudo
     CDS             complement(1..508)
                     /gene="ybfC"
                     /locus_tag="ACHKUB_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
     gene            complement(505..>1329)
                     /locus_tag="ACHKUB_005253"
     CDS             complement(505..>1329)
                     /locus_tag="ACHKUB_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6658107.1"
                     /translation="VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAE
                     YDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKG
                     GWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPY
                     PIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYL
                     PQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          319 a          315 c          276 g          419 t
ORIGIN      
        1 tttgttcggg agatgtgctg tcattcgagc ataagttggt ttctcatctt taaaatattt
       61 taccatgatt gaattatcaa ggactgccaa caactgaact ggatcactgc tctttttttg
      121 atagataccc gctcttatat aacttctaaa ccgatcgccg agaaacgccg ttttttctgc
      181 tgtatttatc aactcctgca acgttccttt gatggcatga ttttcattaa ggcgtagtga
      241 cccgacgcga attaagagtc tcaccccgct tggtgtgaat ttattatttt tacatgtacc
      301 cagttctgtt tgccaataat actcaccgat gtcctcattc tgataccatc tccacatggc
      361 tttattattt aagacaggaa acgcggaaaa aaatagtacc cttagcgaaa atagtaaaac
      421 aatcatgatc tttagcttgt atttcgcaat cagctataac accaaaacta acaaatatca
      481 tcagcaaaaa gaacaaaact cttttcattt tcttattcct ctcgatgttc tctgtttcat
      541 cttagctaaa atttcaccca aatcatctat ccctgctgtt gctggcccat cgggtttatt
      601 aatgtctggg aataaatagg gttctgcaca agtatatcct ggtggtaatt gagttgttgg
      661 ccaatttgtc tgtggcaggt aatcacttgc tgtctttgta tccatccctg gatactggga
      721 atcatcctgt ggcgtacaag tcagatttgg ttttacccac ttcctgcaat acagtgtgta
      781 gtcgtaaggg cttaatgctc tgaagaatgt tttgaaaaca ttaggcgcac tactggcctg
      841 gtcaatagga tatggcagtc ttaattgttt cgctatgtcc ggcaatttac ttggatcggg
      901 cgtaggcggt ttgttgggtc tcgctccata tccgccatta tttaagtgtc catattttga
      961 tgccagtccc attgaatcta tatactgaac cggattcagc ggatactgat aaaaattcca
     1021 tccccccttc agcccaatcg gatcctgagt gatatacctc ccctgcagcg ggtcataata
     1081 gcggtggcgg ttgtaataca ggccggactc ctcatcatac tgctgccccg gcaggcggat
     1141 aagctgctgc agctgatgcg ggttctcttc attcagcagg ttgccccatt catcgtattc
     1201 tgcgcaccat tctgttgccc cttccgtgct gacaagcgcc agcggcaggc cgcgatggtc
     1261 gcagtggtac aggtggattt ttcgcgccgg cgtgtacacc gggtccatct ggttttgcat
     1321 ctgctccac
//
LOCUS       DAZFRN010000374         1310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFRN010000374 DAZFRN010000000
VERSION     DAZFRN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.378"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(136..570)
                     /locus_tag="ACHKUB_005254"
     CDS             complement(136..570)
                     /locus_tag="ACHKUB_005254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFO6658108.1"
                     /translation="MVDSSVDPENAIIAGWVNVITMGQGWKGTVGWNAAGGALGNWID
                     DKDPLSGALINGTGSGIGYGLGKGLSWGVNAGANWWKGGWDPKFNSELRSVTEVQGDL
                     GISKEMKPSNVPSSIGDIGGSITSEAGGKYTEYKMKQEGGGK"
     gene            complement(1034..>1310)
                     /locus_tag="ACHKUB_005255"
     CDS             complement(1034..>1310)
                     /locus_tag="ACHKUB_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658109.1"
                     /translation="SALNFLLLSEQWSYAFEYLTSNADNITLAELEKALFYFYCAKNE
                     TDPYPVPEGLFKKLMKRYEELKNDPDAKFYHLHETYNDFSKAYPLNN"
BASE COUNT          372 a          352 c          216 g          370 t
ORIGIN      
        1 gaaaaagaca ctggtattcc tttataaaaa aagaaaaaaa attccccgca tacatataaa
       61 accgcccctg ttagaaaaat tgaaaccatg ctgaatacaa taaaaaaaac agaggaagaa
      121 tgaattttag agctttcatt tgccaccacc ttcctgcttc attttgtact ctgtatactt
      181 acctccggct tctgaagtga tactaccacc aatgtcacca attgagctgg ggacattgct
      241 tggtttcatt tcttttgaga ttcccaaatc tccttggact tcagtaacac ttctcaactc
      301 agaattaaac ttcggatccc aaccaccttt ccaccagttt gcccctgcat taacacccca
      361 tgaaagtcct ttaccaaggc catagccaat acccgaaccg gtaccattga taagtgcacc
      421 tgacaatgga tctttgtcgt caatccagtt acccagtgcc cctccggcag cattccatcc
      481 aacggttcct ttccagccct gccccatggt gattacattg acccatcctg caatgattgc
      541 attttcagga tcaactgaac tatctaccat gtaaccaatt cctgcatttg cacctgtgcc
      601 aagagcccac atggcctgag caccgccagg taagaaagct acactaccag tggccaacgc
      661 tccggcatac gctttattga ctgcttctcc tcgcttacag gcttcaccgg aaggattatc
      721 actacaggag agaacatcag cgccatgctc tctggctttt tccgtctgga gccattcact
      781 gccacccagc aggttattct caacaacaac tctttccgcc gcaggttgct gtgataaaaa
      841 aagttttccc tgtgctttca gcgcgttaaa accgctttgt cctgctataa ttccggcgca
      901 acaacttttt gccctgacgc gccctttcag ccagttacac catttccccc aaatctgtta
      961 aagaatagct cccaacacac tatgaccatc atccttccat gcagtcatag acaatattcc
     1021 atggcgatag cttctaatta ttcagaggat aagcttttga aaaatcattg tacgtttcat
     1081 gaagatgata aaattttgca tcagggtcat ttttcagttc ttcatatctt ttcatcagtt
     1141 ttttgaataa cccctctggt acaggataag gatctgtctc attttttgca cagtaaaaat
     1201 agaaaagcgc cttttcaagc tctgcaagag ttatattatc cgcattgctt gtcaaatact
     1261 caaaggcgta agaccactgt tctgacaata ataagaaatt cagcgccgaa
//
LOCUS       DAZFRN010000375         1296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFRN010000375 DAZFRN010000000
VERSION     DAZFRN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.379"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(143..952)
                     /gene="pduF"
                     /locus_tag="ACHKUB_005256"
     CDS             complement(143..952)
                     /gene="pduF"
                     /locus_tag="ACHKUB_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFO6658110.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKLAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLVSIFSTYPAAAINVWQAALVEVVITSMLMGV
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT          447 a          314 c          242 g          293 t
ORIGIN      
        1 acatcatccc tgatgattta ctgacgagaa gcacaaagga caataacaaa agcctccgtg
       61 aagcgggaga ctttttaata agttcggaaa gcagatcaca aacagtaaaa aaataaatgt
      121 ttataactgc cagagtattt cgttattcgc taattccttt aggtgtaaca ctattgacag
      181 gtaaattctt tgcaatgaga tagcgataaa tcgccgcacc aacacatgcc ccaatgatcg
      241 gtgcaataat gggtacaatg aaataaggaa tattgcgtcc accagtcatc gcaatttctc
      301 cccaccccgc aagccaggtg aaaatcttag gtccaaaatc acgcgctgga ttcatggcaa
      361 aaccggttaa tggtccggta gaagcaccaa taacagcaac cagaatacca ataagtaaag
      421 gtgcaagcgg tcctttgggt acgccattac cgtcatcggt caacgccata atcaccccca
      481 ttaacatgga cgttatgacc acttcgacca acgcagcttg ccatacatta atcgccgctg
      541 ccggataagt actgaagata ctgacgagtt gtaaactttc tgcgctacca cgcaccatat
      601 tgtgagcaga ttcaaaatcg gtaaacagac tactgtatag agaatatgcc agaaccgcac
      661 caccaaaggc acccgctaat tgcgccacac aaaatggcag cactttacgg gctgggaaag
      721 aggcaaacag ccacagagca atggtaacag ccgggttgag atgagcgccg gaaatacctg
      781 ccgtaaggta aacagcaagc gaaatcccta acccccaaat gatacaaatt tcccacagtc
      841 ccagacttgc cccagcaagt ttcagggcgc acaaacaacc aatgccaaaa aagaggaaca
      901 gtccggtgcc taaaaactcg gcaacgcatt gcgccttgag tgaatcattc atagtgacac
      961 cttctttacg gagtttttct ttatcagcca actatgctct gattgttgtg tataccgagc
     1021 gcaactataa aaaattgctc tgcagaaaat attgccaaca tgcatgacag actcgtgtaa
     1081 aaaatcgtgc taacataaca aattattgtc attgccgaat catgcaacac agcgttaatc
     1141 agtgttgaaa gtaataatta ctgtcaaact ttcacaataa aaaattgcca tcatgatata
     1201 ataaaattga aacaaatatc acagttcctt cctcgaacta aactcaacga aatattactc
     1261 gcgattaaac tcactagcca acgcaattaa ttcaca
//
LOCUS       DAZFRN010000376         1296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFRN010000376 DAZFRN010000000
VERSION     DAZFRN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.380"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            62..198
                     /locus_tag="ACHKUB_005257"
     ncRNA           62..198
                     /ncRNA_class="other"
                     /locus_tag="ACHKUB_005257"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(391..>1296)
                     /gene="ycdU"
                     /locus_tag="ACHKUB_005258"
     CDS             complement(391..>1296)
                     /gene="ycdU"
                     /locus_tag="ACHKUB_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO6658111.1"
                     /translation="IGFYWKNAGFTFPSEDIGVVSGILALVLYNFIFDLKAYWAYKCV
                     TKNIDFSWFKKKQNHKIELFLTQPLVAGFLSLIMLSAMSWGLYQLLPSLYALFLISLL
                     GPLVIFLLFRMIRTSYVKQVAISVAKKVKYKSLTRYVLLSVCISTVVNLLTISPLRNS
                     DSFVTEGQWLTFKSIIALLILCGVVLAINLFFLRFSKRYAFLGRLFLQEIDLFFSSEN
                     ALSTFFAKPLWLRLFILLVIEMMWITLVSVLATLVEWRIWFEAYFLLCYVPCLIYYFF
                     HCRCLWHNDFMMACDMYFRWGHFNK"
BASE COUNT          428 a          322 c          262 g          284 t
ORIGIN      
        1 tccccccctc accgccatat ttaaagaaga gctcgtacga aagtacgggc ttttttttcg
       61 tatattgcac acaccggggg gatgagaagc cccgaccggg gttcgacaac tggcgacagc
      121 cagttggaca gaccgtgaac gcagtgagcg ggctgcccgc agggcgagcg aagcgagtca
      181 atccccccct caccgccata tttaaagaag agcccgtacg aaagtacggg cttttttttc
      241 gtatattgca cacaccgggt gcccccgccc acgtctgtta gggcgagggg aaattgtgca
      301 catcaactgt ctggttactc agtcaacaat caaaatgcgc aagatagtag agaacatata
      361 tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc aacggaaata
      421 catgtcacat gccatcataa aatcgttatg ccagaggcat cgacaatgga aaaaatagta
      481 aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc gccattctac
      541 aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataacca gcaatatgaa
      601 taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gcattttcac tggagaaaaa
      661 cagatcgatt tcctgcaaaa aaagcctgcc cagaaaagcg taccgcttgg agaagcgcag
      721 aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa ttatcgattt
      781 aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgtaacg ggctgatagt
      841 aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag tcagactttt
      901 atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg tgcggatcat
      961 ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca gcgcatataa
     1021 cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta acgacagaaa
     1081 tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct ttttcttaaa
     1141 ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct tgagatcaaa
     1201 aataaaatta tacagcacca gggccagaat cccactaacg acaccaatat cctctgatgg
     1261 aaaagtaaat cctgcatttt tccaataaaa gcctat
//
LOCUS       DAZFRN010000377         1287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFRN010000377 DAZFRN010000000
VERSION     DAZFRN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.381"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..246)
                     /locus_tag="ACHKUB_005259"
     CDS             complement(<1..246)
                     /locus_tag="ACHKUB_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO6658112.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKE"
     gene            complement(1080..>1287)
                     /locus_tag="ACHKUB_005260"
     CDS             complement(1080..>1287)
                     /locus_tag="ACHKUB_005260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658113.1"
                     /translation="AKNNISQAASNPDFYIINNACFFHHQSVNQLNIFQGYFTLARQK
                     LSGMQDTLSGRAVVNAGTCFPKVI"
BASE COUNT          396 a          265 c          279 g          347 t
ORIGIN      
        1 ctcttttacc gggaatggcg aaccatcctg agtaaaactg aatttggcaa attcgctgtc
       61 gctgaggcct cctacaaatg ccactccgga atcaacaaca gatacgacaa caggggtatt
      121 cttgtctgtg agcgacatgg ttgtttctgt caggccaatt gcatcaaaat accagggttt
      181 ttctgccctg acagacacag atataaaaag caggaacaga atatgcggcc ataaaatctt
      241 aagcacaata tcctccagtc agtacaaata aacagtattg caggtcaata tcttacagga
      301 acatcacatc tgaaatatcc tgttttcgac aaaagataaa caatcacaac caataaagcg
      361 agtgaagccc gtctcaaaaa cattaatttt tatatttatg tatccaatga attaacgctc
      421 accttcacgg taatatgcat ctggatttct gcaacaggtc agcgataaga tgctctgcct
      481 cctccttcag agcagaagta aatacagcca tgatggataa aacttaacca gaaaaaataa
      541 gaggaggcaa gtaagttgcc tggcatgttt atgacaataa aaagttccgt caggcatcat
      601 ggcgaacagg atatcacagc gccgttttac cggggcaatc agtgaatggt ggtgtggttc
      661 attggtaagc cccggatatc aggtcactga gtatattcat gaatgaccgt taaaccgggg
      721 atgcgagctg ctgcccggga aagtcgaact gaccggtcaa tagatatcaa tacggggcag
      781 tcagtccact tacaggtatc tgacaaggac accttctgag cgtgcaatgt ggacgggtaa
      841 tacaacgatg gtagcaggct ggtcagcaat cagcagtata tgcccatatt tcctgaggcc
      901 ggatatctgt ttcctgagct tgttttcgac gtttgctaat attttgtcga actaattatc
      961 agaacgatta atgataacag tgtggcaggg atctttaccg atatcaacgt aaataatgac
     1021 cttaacaggt gcgtaattgc tggattttgc catgctgtta ccctgttgat acgggttaat
     1081 taaataactt tggggaagca ggtaccggcg ttcactaccg ctctccctga taatgtatcc
     1141 tgcatgcctg ataacttttg ccgggctaat gtaaaataac cctgaaatat attaagttga
     1201 ttaacagact ggtgatggaa aaaacaggca ttatttatta tataaaaatc cggatttgat
     1261 gcggcttgcg atatattatt ttttgcc
//
LOCUS       DAZFRN010000378         1244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFRN010000378 DAZFRN010000000
VERSION     DAZFRN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.382"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            101..859
                     /locus_tag="ACHKUB_005261"
     CDS             101..859
                     /locus_tag="ACHKUB_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO6658114.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(912..1019)
                     /gene="yniD"
                     /locus_tag="ACHKUB_005262"
                     /pseudo
     CDS             complement(912..1019)
                     /gene="yniD"
                     /locus_tag="ACHKUB_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
BASE COUNT          316 a          282 c          291 g          355 t
ORIGIN      
        1 tttataattc gttacctttc cagggacgct aatctttgat ccttaagaaa gtttccggga
       61 ccgcgatggt ctcttttttt gttgtacgga ctcttgataa atgaagcttt tgaagacagt
      121 tcctgccatc gttatgttgg cggggggcat gtttgcgtca ctgaatgcag ctgccgatga
      181 ttccgttttt actgtcatgg acgatcctgc cagcgctaaa aaacctttcg aaggtaatct
      241 gaacgcagga tatcttgcac aatcaggcaa tacgaaaagc tcctcactta cggctgacac
      301 caccatgacc tggtatggac aaaccactgc ctggtcgctg tgggggaacg ccagtaatac
      361 ttcttctaat gatgagcgtt cttcagaaaa atatgcggcg ggcgggcgta gccgttttaa
      421 tttaactgat tatgactatt tatttggtca ggcaagctgg ctgacagacc gttataacgg
      481 ctatcgagag cgcgatgtgt tgaccgcggg ttatggtcat cagtttttga atggcccggt
      541 acacagtttc cgctttgaat tcggtccagg tgtgcgttac gacaaatata ccgacaatgc
      601 cagtgaaact cagccgctgg gctatgcctc cggtgcttat gcatggcagt tgactgacaa
      661 cgctaaattt acgcagggtg tttccgtctt tggcgcggaa gacacaacgc tgaactccga
      721 aagcgcatta aatgttgcta ttaatgaaca ttttggactg aaagtggctt acaacgtcac
      781 atggaactct gaacctccag agtctgcacc ggaacatacc gatcgtcgga caaccttatc
      841 gctgggctac agcatgtaaa attatctgcc ggaccgtgtt atgcggtctg gctgtcttcg
      901 tttgtcactg ctcagcccca aatcattgct gcccaacgta gcaacaacat agcgccacag
      961 attgccagta gcaccaccac catctttcag tgttttttat caaagcgttt agtcggcata
     1021 ccagtgtcct gcatatctct tgtgcgtgat aacggagtgt aacaaaaggg acaggcatca
     1081 agggattttt tgttattaca gccgtcgggt ggcgttgtac acctgatcac tgtttctgcg
     1141 ttttatgaaa aaaataaaat tatgtatcga tatacaaata tcataaatag ctggttataa
     1201 atacgttttt agttcatcag cactaaaact caggctttgg ctga
//
LOCUS       DAZFRN010000379         1241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFRN010000379 DAZFRN010000000
VERSION     DAZFRN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.383"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            133..636
                     /gene="ftnB"
                     /locus_tag="ACHKUB_005263"
     CDS             133..636
                     /gene="ftnB"
                     /locus_tag="ACHKUB_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO6658115.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(715..966)
                     /gene="yecJ"
                     /locus_tag="ACHKUB_005264"
     CDS             complement(715..966)
                     /gene="yecJ"
                     /locus_tag="ACHKUB_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO6658116.1"
                     /translation="MSQPLNADQELVSDVVACRLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1081..1167)
                     /gene="azuC"
                     /locus_tag="ACHKUB_005265"
     CDS             complement(1081..1167)
                     /gene="azuC"
                     /locus_tag="ACHKUB_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO6658117.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT          334 a          289 c          279 g          339 t
ORIGIN      
        1 tttcagatca tcgtcagaat tgactccacg atcacatttc ggaccggcag aaaggaatta
       61 ttctgcaaac agtaattatg gtgttttgat ttatcttgca cctctccact tctggatata
      121 aggatattag gtatggcaac cgctggaatg cttctcaaac tcaactctca aatgaaccgc
      181 gagttttacg catccaatct ctaccttcac ctgagtaact ggtgttctga acagagtctg
      241 aacggcaccg ccactttcct tcgcgcccag gcacagagta atgtgaccca aatgatgcgc
      301 atgtttaact ttatgaagag tgtcggcgct acccccatcg ttaaagccat tgatgttccc
      361 ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa cgatggaaga atacgagcaa
      421 cgttctagta cgttggcaca gttagccgat gaagcgaaag aactgaatga tgattcaacc
      481 gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg atggtctgtt gctgcaaacc
      541 attcttgatg aagtgcgcag tgcgaaactt gcgggtatgt gccctgtgca gaccgaccaa
      601 catgttctga atgtcgtgtc acaccagctg cattgatcat catcggcgct aatgcattgc
      661 gccgatgaag gttttgagaa accgctgcct catctgtttg aagcagcggt ttttttaatg
      721 ggattcaccc tgtggggtaa acttgagttc aataagcgcg atggcttttt ggattgcccg
      781 catggtgacc gggtctgcgg cggcgggatg gttagtaaag tcgatattct tcagctgact
      841 ggacattttt tcacgaactt caacgggcgc gattacatcg agaacatcca gaatttgttt
      901 gataaccaac cggcaagcaa ccacatcaga aaccaattcc tgatcggcat tcagcggctg
      961 ggacatcgta aactcctgat agcattttga aagccgttat agtagcgact tcacatcttc
     1021 agcgatagtc acatccaccg tcatcaggac acaaaaaaac ctgccggagc aggttttttg
     1081 ttatcggaac atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt tattgaacat
     1141 acttttcagg attttgcgca gtttcatcgc ggcactccga ccatttgtta tacaggtgtt
     1201 attgtctttg cgcttataat atgacaacca tcacaaaaat c
//
LOCUS       DAZFRN010000380         1221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFRN010000380 DAZFRN010000000
VERSION     DAZFRN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.384"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1221
                     /locus_tag="ACHKUB_005266"
     CDS             <1..>1221
                     /locus_tag="ACHKUB_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO6658118.1"
                     /translation="MGPKGEKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPR
                     GEPGPAGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKA
                     ATPKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEF
                     VAWLKEKGAFDQPYWMMKASLPAAMNKVITDVGPGKLNLGGCVIEVMGTYEAAIVRVT
                     IGEYGATGFINGTVCTCTVYGDTRYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSS
                     DTGSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGET
                     GATGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGNKQTYAP
                     QTDSTTWAVDLDIGA"
BASE COUNT          334 a          269 c          440 g          178 t
ORIGIN      
        1 tatggggcca aaaggcgaga aaggtgatcc gggaggtcct gcaggcccgc aaggtcctaa
       61 aggtgaacga ggagaagccg gaccacaggg accgatggga gcacgaggtg agcgtgggga
      121 gactggcccc cgaggtgaac ctggtcctgc aggtccgaga ggcgaacgag gagagaccgg
      181 acctcaggga cctcgtggag agccaggtcc ggcaggcagc gctgcaaatg tggctgatgc
      241 gacgacggca cagaagggaa ttgtgcagtt aagcagcgca acggacagtg atgatgaaac
      301 gaaggcagcc accccgaaag cggtgaaagc ggcaatggac aaagcggacg gatgcctgga
      361 gaaagcgaaa aacggtgacg atatcccgga taaggtgaag tttctgaaca ccgtgggggc
      421 agccagagta tacgggcggg acattcatac gggggccggt gaatggacca cgagtgagtt
      481 tgtggcctgg ctgaaagaaa agggggcatt tgaccagcct tactggatga tgaaggcatc
      541 actgcctgcg gcaatgaata aagtcatcac agatgtcgga ccgggaaaac tcaatctggg
      601 tggctgtgtc attgaggtga tgggaacgta tgaggctgcc atagtccggg tcaccattgg
      661 cgaatacggt gcaacggggt ttattaatgg cacggtctgt acctgtacgg tttacggcga
      721 cacacggtat ttccactggc gggtggatta cagcacaaaa aacaaaccgg atacggtcag
      781 ccagcgggat gccagcacga cgcagaaagg tgtggtgcag ttaagcagtg atactggcag
      841 taatgacgaa acaaaggcag ccacgccgaa ggccgtgaag gcggcaatgg atgtggcaaa
      901 tgaagcgaaa acaaaggcag aagaggctgc agcaggaggt ggtgttcccg gtccgaaagg
      961 agagaaaggc gaccccggag cacagggtcc gaaaggtgaa acgggagcaa caggtcccgc
     1021 tggtccgcgg ggagcgcagg gaccgaaagg ggagaaaggc gaccccggtc ccagaggtga
     1081 gcgtggagaa accgggccga tggggccgcc cggggcatca gacgggaaga gccgggttgt
     1141 gggtatcagg ctgggtaata aacagactta tgcgccgcaa acagacagca ctacatgggc
     1201 agtggatttg gatattggtg c
//
LOCUS       DAZFRN010000381         1214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFRN010000381 DAZFRN010000000
VERSION     DAZFRN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.385"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(19..1155)
                     /locus_tag="ACHKUB_005267"
     CDS             complement(19..1155)
                     /locus_tag="ACHKUB_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO6658119.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
BASE COUNT          360 a          292 c          215 g          347 t
ORIGIN      
        1 cgtttaaatg gatacctgtt aaaactgata catcatacca acagcaagta tattatcggt
       61 tgcaactcca gcctttttgg taaattcgct ttcatcaata aggttaattt tgtaatcaat
      121 aagaagtgac atgtttttgt tcagaaagta gtttgcgcca acatcaatat actcaacaag
      181 agactgatcg ccatagttta ctattccacc tttaacatcc atccctttgg actgaacgta
      241 agccagtgaa ggacgcaggc cgaaatcaaa catatactga gccacaactt caatattccg
      301 tgctttatct gcgatgcctg cgctaccaaa tggagtcata tttcgggttt cagagtagtc
      361 agctgccaga tacaggtcat tatcattata cttgacccca acacctgcaa actccgcatg
      421 ttttcccccg gcaatccagc gaggcgtaat aacttctttt ccttctgaat ctttcgatgt
      481 catgaaacgt ttacggcctt ctttttcctg cagactggtt ctgtcggcat tagtgtaagc
      541 ggcaacgatt ccaaaacctt catagtcata gcttacggag aaaccatatc catcaccatt
      601 agcttcaagt aaattatcac ggtcattttt accctgatat tgagcagaaa aagtaagtcc
      661 atcaacaagc ccgaagaaat ctgtattgcg ccaggtcaga actccggttg ttctgccggt
      721 catatagtta tcagagtaag tccagctgtc accaccaaag tccggaagta cgtcagtcag
      781 agcccccaca tcataggcaa caccatagtt tcgcccataa tcaagtgaac cgagtatatt
      841 gtggcgaata cctgcaaatg ccagacgatt tttatcgttg cgtacttcac tttctgtctt
      901 aaaccccagg aactggtatt cccactgacc aaagccagtc aggctctcat taatttgtgt
      961 ttcgcctttg aaaccaagac gtgcaaatgt tctgtcgcca tccatagaac catcagagat
     1021 gtaatgacgg gctttaacgc gaccataaaa atccagttta ttaccgtctt tattatagat
     1081 ttctgctgcc tgtgttgcct cacacagcat taatgcagaa acgacagagc acacaaccat
     1141 acttaatttt cgcataaaaa aatcccctgt gcacattttt agtatggttt taaaaattaa
     1201 actatggcga aaaa
//
LOCUS       DAZFRN010000382         1205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFRN010000382 DAZFRN010000000
VERSION     DAZFRN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.386"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..102)
                     /locus_tag="ACHKUB_005268"
     CDS             complement(<1..102)
                     /locus_tag="ACHKUB_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6658120.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYQ"
     gene            complement(113..811)
                     /locus_tag="ACHKUB_005269"
     CDS             complement(113..811)
                     /locus_tag="ACHKUB_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6658121.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGCSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSALTTMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(811..1140)
                     /locus_tag="ACHKUB_005270"
     CDS             complement(811..1140)
                     /locus_tag="ACHKUB_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6658122.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVSREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1137..>1205)
                     /locus_tag="ACHKUB_005271"
     CDS             complement(1137..>1205)
                     /locus_tag="ACHKUB_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6658123.1"
                     /translation="KGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          249 a          438 c          275 g          243 t
ORIGIN      
        1 ctggtaccgt tcgccctccg gggtgcttat cacgaagccg cacgactccg ctggcgcaca
       61 ccgccgggca tgcgccagaa tcgctgattc agtctgtgtc ataaaacggg atttactgcg
      121 aaagtttatt aatggaaagg aaaccgccga aattagccac catgccgcgc atctcacacc
      181 cgcgcatgca tttactgcat ctgtccttcc ggatatccgt ggtggggttg tcgaactcat
      241 ccgccactgc cggaccgtta tacccgcatt catcgccccg gtaatcccac atacaggtgt
      301 tcgccagcat gatgcgaccg ggaaacagcg caccgtccgt ctccgtcggt gtcgccagca
      361 caaacgaggc cgtcatggtc gtcagcgctg acatctgctc caccacccag cggtccgtca
      421 gctcctgctc cgggtctgcc tcaggattgc ctgccacaaa gttcaccgca tccagaaaac
      481 gcgcatacac ccggcggcgg accaccgtgg cacccaccag gctctgcaaa tcctccgcca
      541 tcccagtgac aaggccgaaa agattggaca ccgtcagcga cgggcgggca ctgcagccct
      601 ttccgttcat ctcaaagccg ctgccctcaa tcgggtacgc ctcatacttc cgcccctgcc
      661 aggtcaccgg ctcccctttt tcattcagct cattgcagaa aaaataccgc tcaccgccct
      721 gcaccgtcag gtcgatttcc cagagcacca cccgcggtga ctgctctgac ttaaccgact
      781 cgttcaggct tgcttcgtga atatcctgca tcagttcacc acctgcttaa actccgcgct
      841 gaactcaacg cgcaacatcc cgacccgcgc agaccacccg gcacaggtca cctttatctg
      901 ccggtatgca tagggtggct tccacaaaaa tgccttccag cccccgtgct ctgccaggaa
      961 cgcttccaga tgccgggcct cctcccggct cacggaaagc atcacccggt atgttttcag
     1021 gtcagcattc agccctgccg ccatacgctg tgagtacccg tcaccaaaac gcacttcacg
     1081 caccgatggc tgcgagttca cctccatatc cggcttcact ttccagcgaa aggttttcat
     1141 cgcccgctcc ctgataacat accgccatca cgcaactgca gccggagctc atcctgcgcc
     1201 ccctt
//
LOCUS       DAZFRN010000383         1198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFRN010000383 DAZFRN010000000
VERSION     DAZFRN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.387"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACHKUB_005272"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACHKUB_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO6658124.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKALDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHKUB_005273"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHKUB_005273"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO6658125.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          319 a          274 c          256 g          349 t
ORIGIN      
        1 ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca attttacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat ccctgatttc
      421 gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttccgcgggt
      481 aaaaagctga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      541 aaggacttac aaagcaaagc gcttgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaaa taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      721 attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc tttcaaacgt
      781 aacgtttttc gggtggcaac atttaaatca taatgcacca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      901 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcataggggt attcctta
//
LOCUS       DAZFRN010000384         1192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFRN010000384 DAZFRN010000000
VERSION     DAZFRN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.388"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(42..929)
                     /gene="rhtA"
                     /locus_tag="ACHKUB_005274"
     CDS             complement(42..929)
                     /gene="rhtA"
                     /locus_tag="ACHKUB_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO6658126.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT          353 a          303 c          287 g          249 t
ORIGIN      
        1 aaattctgca tggttatgca taaccatgca gaaatgtaaa tttaattaat gtctaattct
       61 tttattttgc tctctttgcg tactgtcagc gtagacccca ttgaagcggc gatgatagcg
      121 ccgagcgcca gcagctgtat gggtgtcagt gtttctccga ggaaaatcat cccggaaacg
      181 gcagccagcg ccggttccat gctcatcagc gtaccaaatg tccgtgttgg caaacgggtg
      241 agggcaatca tttccagcga ataaggcaga gcggtcgaga gaatagcgac agccagaccc
      301 aatggaataa ccgaccagtg ccagagtgct tcaccagcct gaagcgctcc aattggcacg
      361 aaaattaacg ctgcaatcaa cgaaccaatt gccaccgtcg cagggccatg ttccgctcct
      421 gcgcgttgcc cacttaaaat gtaaatagcc caacaagccc cggcccccag tgccagcgca
      481 cagccggtta aatcgacatg ggaaacgtct tgccccagcg gtagcaggaa ccacagacca
      541 agaaccgcca gcacaaccca gacgaaatct accgggcgac gagaagagaa cagcgccacc
      601 gccagtggtc cggtgaactc cagcgccacc gcaataccca gcggtactgt ctgaatagaa
      661 agataaaaaa gataattcat cccacccagc gaaacgccgt aaaacaacag cggtaaccgt
      721 tgctctttgg caaagcgcag tcgccatggc ttaaagaacg cgatgaggat cagcgttcct
      781 aatgccagac gcagcgcagt gacacccggt gcgcccacca gaggaaaaag tgacttagct
      841 aacgaggctc caccctgaat agacgccatg gcaacgagca atatgattat tggtaaccag
      901 accggcattt tacgtaatga accaggcatc ctttctccca caaatatcta gacttaagta
      961 aagcgtggag tgtactggat atacccaatg ctggttgagc atttgttgaa aaaattttcc
     1021 cccgttttga ctaaaatgcg ccaggattga tggaatcatt agtctggtga ttaggaataa
     1081 tctggatgaa tgacagggaa aacatgcgta atacttacgc agttctctga aaaagtgatt
     1141 taaatttaga tggatagcgg tgtatggaaa cgttctgtta catgaaatgg cc
//
LOCUS       DAZFRN010000385         1190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFRN010000385 DAZFRN010000000
VERSION     DAZFRN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.389"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1190
                     /locus_tag="ACHKUB_005275"
     CDS             <1..>1190
                     /locus_tag="ACHKUB_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFO6658127.1"
                     /translation="RSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTTGK
                     PVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTAGA
                     NRVTADGRVRALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARDGD
                     ITLDASGRLTVNNSLATGAVTAKGQGVTLTGDHKAGGNLSVSSRRDIVLSNGTLNSDK
                     DLSLTAGGRITQQNEKLTAGRDVTLAAKNITQDTASQINAARDIVTVASDTLTTQGQI
                     TAGQNLTASATTLTQDGILLAKSHAGLNAGTLNNSGAVQGATLTLGSTTLSNSGSLLS
                     GGPLTMNTRDFTQSGRTGAKGKVDIMASGKLTSTGLLVSDDALVLKAQDVTQNGVLSG
                     GKGLT"
BASE COUNT          286 a          332 c          363 g          209 t
ORIGIN      
        1 cgttcactgc tgcagggcta tacggaagtg gccggcaaag cggcgaatgt gatggttgcc
       61 aacccgtatg gtatcacctg tgacggctgt ggctttatca acacgccgca cgcgacgctc
      121 accacgggca aacctgtgat gaatgccgac ggcagcctgc aggcgctgga ggtgactgaa
      181 ggcagtatca ccatcaatgg cgcgggcctg gacggcaccc ggagcgatgc cgtatccatt
      241 attgcccgtg caacggaagt gaatgccgcg cttcatgcga aggatttaac tgtcactgca
      301 ggcgctaacc gtgtaactgc agatggtcgt gtcagagccc tgaagggcga aggtgatgtg
      361 ccgaaagttg ccgttgatac cggcgctctc ggtggaatgt acgccaggcg tattcatctg
      421 acctccactg aaagtggtgt cggggttaat cttggtaacc tttatgcccg cgatggcgat
      481 atcaccctgg atgccagcgg cagactgact gtcaacaaca gtctcgccac gggggccgtc
      541 actgcaaaag gtcagggcgt caccttaacc ggcgaccata aagcgggagg taacctgagc
      601 gtcagcagcc ggagagatat cgttctcagc aatggaacgc ttaacagcga caaggacctc
      661 agcctgaccg ccggcggcag aatcactcaa cagaatgaaa aactgactgc cggccgggat
      721 gtaacgcttg ccgcgaaaaa catcacacag gataccgcca gccagattaa cgcggcccgc
      781 gatatcgtga ctgtcgccag tgacacgctg acaacacagg gacagataac cgccgggcag
      841 aatctcacgg ccagcgccac cacgctgacg caggacggaa tattgctggc gaaaagtcat
      901 gcgggactca atgccggtac gctgaataac agtggcgccg ttcagggagc taccctgacg
      961 ctcggcagta caacgctcag caacagtggc tccctgctca gtggcggtcc cctgaccatg
     1021 aatacccgcg actttaccca gagcggccgc actggcgcga agggcaaagt ggatatcatg
     1081 gccagtggga aactgaccag tacaggtttg ctggtgagcg atgatgcact ggtgctgaag
     1141 gcgcaggatg tgacacagaa cggtgtgctg tccggcggca aagggctgac
//
LOCUS       DAZFRN010000386         1189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFRN010000386 DAZFRN010000000
VERSION     DAZFRN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.390"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            323..640
                     /locus_tag="ACHKUB_005276"
                     /pseudo
     CDS             323..640
                     /locus_tag="ACHKUB_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          341 a          284 c          295 g          269 t
ORIGIN      
        1 cgtcctaatt acccatatga ttataacgac aaaaatacat ggatttattt atcaaaaaca
       61 gaggggaaag tgttgtatgg ggctattccc gttgatttca atcgttcagt gatgctaaag
      121 ttggtatttg gaaaagacaa aactattgag ggtatagaat ataaacaata ataaaaaatc
      181 tgctaatgta taacagacag ggcacagcag cagataacgg gatactatat aatacctgtc
      241 agtccataga aggaagttat acgaataccg gagtgctaat gaacagaata tttctgtgaa
      301 gatgacttcc ccgtaacgac accacaaagc cattgtctat gcgctgtttc atcgcgtgga
      361 actgagccgc ttcctggaag atggtgcggt gccgctggat aataatgtgt gtgaacgggc
      421 catcaaaaac gtggtcctgg gcagaaaatc gtgtctgttc gccggttcgc agatggcggg
      481 agaacgcgcc gcgcaaataa tgagcttgct ggaaaccgcg aaacgcaacg gtctggagcc
      541 gcatgcctgg ctgacagacg tcctgatgcg tctgccggag tggccggagg agcgactggc
      601 agagttgctg cctcttgagg gatttacctt ctccgggtga gtgatacctg ccgtcaggtg
      661 ttcgtgcacc gggccataac ctgcagtcgg gagttgaacg cctgacgaca ggaaatgagc
      721 cagaccagca acctcacggg ctgacagcga ataagaacct gaatcagccc ggagtcattc
      781 ccgggtcagt acatcatcac ctgccattaa tccccgagtc agtacttcgc ggcctgacgc
      841 gatcaccctg tgcaacaaaa taaatcaacc gttatcagac cacgatcgtt cgtatgcgcc
      901 gccgatgacg cgcgcctgaa aaacgcgtca agatgagatg cccggaagca ctctgctgac
      961 tgggatcaaa cgccagaaga gcagaggtaa gcaaaaaagt ccgccaacta aggacgggat
     1021 taaatttatg ctttagggac tgatggcgga gattttctgg ctttacgttc atctatccat
     1081 gcaaagatcc atgatcttaa cccagccccc ccccctgcga tcccactcaa tgttatggct
     1141 gctttaaatt gtttttctga aaaatagaaa caacctcctt cccaaaaat
//
LOCUS       DAZFRN010000387         1186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFRN010000387 DAZFRN010000000
VERSION     DAZFRN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.391"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(142..>1186)
                     /gene="ompC"
                     /locus_tag="ACHKUB_005277"
     CDS             complement(142..>1186)
                     /gene="ompC"
                     /locus_tag="ACHKUB_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6658128.1"
                     /translation="SVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTT
                     YARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDY
                     GRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAA
                     QYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYGNNSLNASGQ
                     NAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQFDFGLRPS
                     VAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKASGVA
                     TDDIVAVGMVYQF"
BASE COUNT          336 a          265 c          255 g          330 t
ORIGIN      
        1 cccccaaaaa taaagggtaa tatataacag aaggtttata tagttagaag caaggttgtg
       61 ctcctaaagg aagtggcttg agggagccac ttatatgttg gggaggcaaa gcctcccgca
      121 acatatcttt tagtaatcaa attagaactg gtaaaccata cctacagcaa cgatatcatc
      181 ggtagcaacg ccagatgctt tcgtgaaatc gctcttatca atcaggttga ttttgtaatc
      241 aacaaaagtg gacatatttt tgttgaagta ataggttgca cctacatcaa tatattcaac
      301 caggtcctga tcaccccacg cacccaagtc ttttccttta gattgcaggt aagcaacgga
      361 cggacgcaga ccgaagtcga actgatattg tgcaactact tcgaagtttt gtgctttgtt
      421 ggcaatatgg ttattaccaa aaacagtcat gttctgggtt tcagaatagg tggtagccag
      481 atagatgttg ttcgcatcat atttcagacc agctgcccat acttcagcat tttgaccaga
      541 tgcattcagg ctgttgttac cgtagataac ctgattatta gtgcggtcag atttagcata
      601 ggttgcacct acaccgaatc cttcatactc ataagtagtg gagaaaccga aaccatcacc
      661 attagcttca gttacgtcag tgcggtcatt tttaccctga tactgagcag caaagttcag
      721 accatcaacc agaccaaaga agtcgttgtt acgataagtt gcaacaccag tggtgcgacc
      781 agtcatgaac acatctgttt gggtccaggt atcgccaccg aattctggca gaacgtcagt
      841 ccacgcaccg atgtcatatg ctacaccgta gttacggccg taatcgattg agccgtagtc
      901 cccgaatttc aggcctgcaa atgcaagacg ggttttgtct ttggaggagc cttgagattc
      961 agcgcggttg cctttgaatt catattccca ctgaccgaaa ccagtcagtt gatcgttgat
     1021 ttgggtttca cctttgaagc caagacgggc ataagtagta tcaccatcat ctgcatcatt
     1081 agaggagaag tagtgcttag cattaacttt cccgtacaga tccagcttgt tactgtcttt
     1141 attataaatt tcagctgcct gagcagacat cgccatcagt actgat
//
LOCUS       DAZFRN010000388         1176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFRN010000388 DAZFRN010000000
VERSION     DAZFRN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.392"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(434..1066)
                     /locus_tag="ACHKUB_005278"
     CDS             complement(434..1066)
                     /locus_tag="ACHKUB_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO6658129.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          311 a          250 c          244 g          371 t
ORIGIN      
        1 aaatatcgtc gtagctgcac tcgatatttt taaaatcctg tatcaagtgc tgaaaatcat
       61 ttcatcgcca cttccaattc ctttctgcat ggtaagtagt gaggacgaaa agaagaatat
      121 gataaatatt gattcgtggt aatcagagag agtcgcgaca tttgtgatgt tattcttaca
      181 actgtaagaa gcgccggtga tttacaagta agggtatgat tacgcttttt aaagcgtacc
      241 ataaaacata acataataaa tgatttgtga tgacacggga ttattgggat ttactttgag
      301 gaagataaaa tgcgtgttta tagaaccgcg agcgccaaat aattttatga ccacaacctg
      361 tcgtattgtg gtcataaaaa attgtcggct ggagggagtt aaatccttta cctcaatata
      421 ctgcctgtca gcattagttc acaaacagtg cttcgctgtc gacgatgatt ccatcttccg
      481 cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt ttctgcgcat
      541 cagcaagtgc attacgagtc tgtttaagcg gcatcagata ttggttttcc attacgccaa
      601 caccggccag gcgaagctct tccattgcgc cctttttatc ccctttcgcc attttttcat
      661 tggcaatttt tatcgcggct tctttttcgg gtgttgctac ataactctca gaaattccga
      721 cagatgcatt aatgacaata tattggtcat cattgagatt ggtcttttta cctggcttag
      781 cgaactttgc ccattcggta ctgtcatctg acaacaaagc tgaagcttca ttggtcagtt
      841 cttttgcctt ttccggatcg ccatgaaaca gagccaggcg cgctacctgc acgtcacgca
      901 tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg gtttgctgaa
      961 cttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat acagttgaag
     1021 atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt acctcattct
     1081 ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag aaatcagaga
     1141 gcaacggtca ttttgaaagg gttatcttac agttgt
//
LOCUS       DAZFRN010000389         1172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFRN010000389 DAZFRN010000000
VERSION     DAZFRN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.393"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..139
                     /locus_tag="ACHKUB_005279"
     CDS             <1..139
                     /locus_tag="ACHKUB_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO6658130.1"
                     /translation="RKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMS
                     H"
     gene            482..1012
                     /gene="yodB"
                     /locus_tag="ACHKUB_005280"
     CDS             482..1012
                     /gene="yodB"
                     /locus_tag="ACHKUB_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO6658131.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          365 a          253 c          216 g          338 t
ORIGIN      
        1 acgaaaatca tctcattttc acatttttat gggtaatgat tcacagcaat ctttattaaa
       61 tgaaatggaa aactggccaa cgtattatcc atatcagttg agtagcgaag aagtggtcga
      121 ggaaatgatg tctcattgag tattctcatg ataacgcctc gatgccgctt tagtaagtca
      181 tcataactgc cactggtcca tccacaaacg ccactgaacg caagctagct acagacacgc
      241 tcatcactat gacgtgtctg tatattaata agctaacccg cattgagtta accaataacg
      301 gattccatac acaatacggc caacagagaa agatacctgt gctcacgcca ttgcttatat
      361 tggctggtta caatgtgcac tatcaatttt ttaaataaca aattcttaaa accacatctg
      421 tataaggatt ttttaaggtt cgtgggatag cttgactgta aaaattacag gagctacaaa
      481 aatgaaccga ttctcaaaaa ctcaaattta tttacattgg ataacgctgc ttttcgttgc
      541 aataacctat gccgcgatgg aactccgtgg ctggtttcct aaaggtagta gtacttatct
      601 gctgatgcga gaaacacatt acaatgcggg tatattcgtt tgggtgttaa tgttttcacg
      661 cctgattata aaacaccgtt atagtgatcc ttctattgta ccaccgccac ctgcctggca
      721 aatgaaagcg gcttcgctaa tgcacatcat gctttatata accttccttg cacttcctct
      781 gctggggatt gctttaatgg cttacagtgg aaaatcgtgg agtttccttg gtttcaatgt
      841 gtctcccttt gttaccccaa acagcgaaat taaagcactg ataaaaaata ttcacgaaac
      901 ctgggcaaat ataggttact ttttaatcgc agctcacgct ggcgcagcac tctttcatca
      961 ctacattcag aaagataata ctctgttacg aatgatgcct cgccgcaaat aagtcgatcc
     1021 cctgacaagg aaatgtgaac catagacatc aacgcgtcat aaatgtttac gcaagtgcac
     1081 aatgcaccct ttccactatc aacaaggagg agcagaaact gtgtaaatcg tacaatggta
     1141 agacttatca aaaatacagg ttaattttgg ta
//
LOCUS       DAZFRN010000390         1163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFRN010000390 DAZFRN010000000
VERSION     DAZFRN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.394"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            33..755
                     /gene="sdiA"
                     /locus_tag="ACHKUB_005281"
     CDS             33..755
                     /gene="sdiA"
                     /locus_tag="ACHKUB_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO6658132.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(822..1046)
                     /gene="yecF"
                     /locus_tag="ACHKUB_005282"
     CDS             complement(822..1046)
                     /gene="yecF"
                     /locus_tag="ACHKUB_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO6658133.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1033..>1163)
                     /gene="yecU"
                     /locus_tag="ACHKUB_005283"
     CDS             complement(1033..>1163)
                     /gene="yecU"
                     /locus_tag="ACHKUB_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO6658134.1"
                     /translation="ADITLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT          298 a          255 c          287 g          323 t
ORIGIN      
        1 gatactcact ctcaggggcg ttgcggttta ctatgcagga tacggatttt ttcagctggc
       61 gtcgcacgat gctgttgcgt tttcagagga tggaggccgc agaagaggtc taccatgaaa
      121 tagagcttca ggctcagcag ctggagtacg attactattc gttatgtgtt cgccacccgg
      181 taccattcac gcgacctaaa gtggctttct acaccaatta ccctgaggcg tgggttagtt
      241 attatcaggc aaaaaacttt ctcgcaattg atccggtgct gaaccctgaa aactttagtc
      301 agggccattt aatgtggaat gatgacttat tcagcgaagc acagccgtta tgggaagccg
      361 cgcgcgcaca tggtttacgc cgcggtgtca ctcagtattt aatgctgcca aatcgggcgt
      421 tgggcttttt gtccttttcc cgttgcagcg ctcgcgaaat acccattctt agtgatgaac
      481 tgcaattaaa aatgcagtta ctggtgcgcg aaagtctgat ggctctgatg cgtttaaatg
      541 atgaaatagt gatgacgcca gagatgaatt tcagcaagcg cgaaaaagaa attctgaaat
      601 ggacggcgga agggaagaca tcagcagaga tagcgatgat tttgtcaatc tctgagaata
      661 cggtcaattt ccatcagaaa aacatgcaga aaaaaatcaa tgcaccaaat aagacccagg
      721 tggcctgtta cgcggccgct actggcttaa tttgatcgct tttctttctg cgtgccagat
      781 gcaaaaaccg gctgaaaggc acgctctcag ccggttttat attactgacg gtaggcttgt
      841 ttaatttgct taaccgtttt ggaaaatact gcagcctggg tttcgtcttc gatcagcgca
      901 agctgttttt ccatctttaa catcacgcgg cccgcgtcag cttgtcccat cgcctgcagc
      961 atcagcgtca gcatcgcttt caggcaggag acttcatttg ccagttcttg attattctcg
     1021 gcagtagaaa aatcaggcgt actcatttat tttcctcgtc atgttgcaat gaaaatttgc
     1081 ggtgaaaaat gttaaggcgg cggagtatac cataagcttt gctaaaaata gcagtggttg
     1141 ttttttgagc gtgatatcgg cag
//
LOCUS       DAZFRN010000391         1157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFRN010000391 DAZFRN010000000
VERSION     DAZFRN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.395"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(454..1026)
                     /gene="hdeD"
                     /locus_tag="ACHKUB_005284"
     CDS             complement(454..1026)
                     /gene="hdeD"
                     /locus_tag="ACHKUB_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO6658135.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          393 a          258 c          224 g          282 t
ORIGIN      
        1 gacttaagct ggcaattgga ttgccagctt tcttttaacg cattgaaatt acctgagtaa
       61 ataacgcatt catgtcttca ggtttttata tagcgtctgg agacacggat atttatgcaa
      121 tgtaatcaat gccagttgca aaaaaatgaa cccggattac ttatttaacc gcccatagca
      181 tcaacgctta cggaactctt tgttcacttt cccactgcac ttttaaaaca ttaaacctga
      241 ctacggaaaa tatcagccat gataatgttt gaaatggcta atttgccatg gagtgaaaaa
      301 aattagatga aattcagtag gttgaaataa tcactagcag gtaattattt caatgatagt
      361 gcgcaattga tctacaacgc tgcgtagcgg agagagtatt aatcggatca tagtcacatc
      421 aagtgactat gatccgggtg acaaccggga taattattgc tgcttaacga acaaactggc
      481 gaagctgaac aggctggcgg cgctaaatat cagttcaatt cccaccagtg tggaaaccag
      541 cgttacagac accatcggcg ttgcaccaag gaatatccag gcaatgacga tatccagcac
      601 accaataacg agctgtagcc agctgccttt cattgaacgc tgacgatacc aactcatcag
      661 gcgaataacc cctgcaacac agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc
      721 cagctccggt gcgcggatga agaaatagcc gatcaataaa taggcgactg cgacgaggaa
      781 accggataat accggccaga aattatgact gcggttgctg aataacccga caataagcgc
      841 aatgcccgag cagattaata atgcacccac tactgtgctt aaaatatcgc cagagacgaa
      901 cgggaaactg atacacagca acccgacgat aaacaacagc acggcaataa actggattgc
      961 tctgcgatgt tttttaagca tctccagatc aaacttcaaa attgttgcct tatctatata
     1021 taacatagaa ccaccctata aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa
     1081 aacgcgtcta agaatgcagt cgatttaata aaaatttcct aattacagta tctgatgcat
     1141 ctgtaactca ttgtatt
//
LOCUS       DAZFRN010000392         1138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFRN010000392 DAZFRN010000000
VERSION     DAZFRN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.396"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            127..367
                     /locus_tag="ACHKUB_005285"
                     /pseudo
     CDS             127..367
                     /locus_tag="ACHKUB_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            444..718
                     /gene="sctI"
                     /locus_tag="ACHKUB_005286"
                     /pseudo
     CDS             444..718
                     /gene="sctI"
                     /locus_tag="ACHKUB_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322788.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            715..>1138
                     /gene="sctJ"
                     /locus_tag="ACHKUB_005287"
     CDS             715..>1138
                     /gene="sctJ"
                     /locus_tag="ACHKUB_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO6658136.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTA"
BASE COUNT          373 a          194 c          241 g          330 t
ORIGIN      
        1 atgtaagatt taatgtgaat ttgaaaaatc aggacacaaa taatagttca tttagctacg
       61 gagataaccg attcgagaag tctcaaggta gcaactggac atttcaggaa taattgagga
      121 ggacttatgg cagattggaa tggttatatt atggatatca gcaatcaatt tgatcagggc
      181 gttgatgatc tgaaccagca agttgaaaaa gcgttggagg atttagccaa ccaatccctc
      241 cgacacgaaa tttcttgccg aatatcaaag tgcattagct gagtatacat tatatcgaaa
      301 tgcgcaatct aacgttgtaa aagcgtataa agatcttgat tcagcaatca tacaaaactt
      361 ccgctaaact ggataaggat tttattatgt cagtaagcaa tatgccacct attgatagag
      421 ctgagtaatc aactgctcat gaaatacagc aagcgaaagt gattgattta aatgatcggg
      481 ttctcaatct tgacaacccg gatgataaaa tgatctccgc ttttgccaac tatgctgtac
      541 aaactgaaaa ttggcagcaa aatgcgttac aagcattaag gtctgataaa gaggggttaa
      601 ctcctgaaaa attattagtc ttgcaggatc atgtgcttaa ttataatgtc gaggtttcat
      661 tggtggaaca ttagcaagaa agattgtcgc tgcagttgag acgttgaccc gctcatgaaa
      721 tatatatcat tattattgtt tatcctgctg ttgtgcggct gcaagcagca ggaattattg
      781 aaccatcttg atcaacagca agctaatgat gtccttgctg ttttacaaag acataatatt
      841 aatgctgaaa agaaggatca aggcaaaaca ggattttcaa tatttgtcga accaacggat
      901 tttgcttctg cggtggattg gctaaaaatc tacaatctac cgggtaagcc tgatattcaa
      961 atatcgcaga tgttccctgc agatgcactc gtttcatctc cacgagcgga aaaagcaagg
     1021 ctatattctg ctattgagca gcggttagag caatctctca aaattatgga tggcattatt
     1081 tcttcgagag tacatgttag ttacgatgtt gataatggcg atagtggtaa aactgctt
//
LOCUS       DAZFRN010000393         1097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFRN010000393 DAZFRN010000000
VERSION     DAZFRN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.397"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            91..960
                     /locus_tag="ACHKUB_005288"
     CDS             91..960
                     /locus_tag="ACHKUB_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO6658137.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLVRAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT          335 a          239 c          244 g          279 t
ORIGIN      
        1 tattttaaca actattacta ccaagcccat ttatctggct tataaattaa aattaattat
       61 ttaattgaga aatggttagg gagaacctac atggccataa gcatcaaggg tgttaatacc
      121 ggtgttattc gcaagagtaa taatttcatt gcactggcgc tgaaaattaa agaacctcgc
      181 aataaagaat cgttgttctt tatgtctgtc atggaactgc gcgacctgct gattgcgctg
      241 gaaagtcgta tgcatcaaaa gcataaactg gatgctgctg cacgccttca gtatgagcaa
      301 gctcgcgata aagtcattaa gaaaatggca gaaaacatcc cagaaattct ggttgatgag
      361 cttaaaaatg ccgatattaa tcgtcgggtt aacacgctgg aacttaccga taatcaaggc
      421 gaaaatctta ccttcgtatt aacgctacat gacggtagca aatgtgagtt agtcgtcaat
      481 gagttgcaga ttgaaatgct ggtgcgagca atcattcatg caattaataa tgcggaaatg
      541 cgtgaactgg cattacgtat tacctcactg ttagatttcc tgccgttata tgacgttgac
      601 tgccaggaaa atggcaatct ggaatatgac acctattccc aaccggaatg gaaacataac
      661 ctgttcgacc attatctggc ggtgctttac cgctttaaag atgaaagtgg caaagaacaa
      721 tttagcggcg cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaaat agaggcgatc
      781 actcgccgaa tgcttgattt cagccaacga ctgaaaaaac tcgcgggtgt gccatgtcag
      841 gtctatgttc gcaccgtagc ggcgaataac gcccaaccgc taacccagga tcaatgctta
      901 cgcgcgctgc atcacttacg tgttcaatcc accagcaaaa ctgcaccaca ggcgaaataa
      961 tctcatcttt gctaatgcga ggctggagcc atccagcctg aaatgactga taacgtgatc
     1021 gttggtcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct caagcattca
     1081 atcagttgca atgcctg
//
LOCUS       DAZFRN010000394         1082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFRN010000394 DAZFRN010000000
VERSION     DAZFRN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.398"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..432
                     /locus_tag="ACHKUB_005289"
     CDS             <1..432
                     /locus_tag="ACHKUB_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO6658138.1"
                     /translation="ALWLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTY
                     EERGSDGKWRKPGRGANEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQE
                     RSGETVSPELTAGAEKRRRRKKKLRTERAEDNPWITSGGWL"
     gene            429..641
                     /locus_tag="ACHKUB_005290"
     CDS             429..641
                     /locus_tag="ACHKUB_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658139.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            641..>1082
                     /locus_tag="ACHKUB_005291"
     CDS             641..>1082
                     /locus_tag="ACHKUB_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6658140.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVF"
BASE COUNT          249 a          244 c          366 g          223 t
ORIGIN      
        1 gcgctgtggc tggttcagac ggatgcgttt aaggaccgtg taaataatgc cctgtggcgt
       61 gacacaccag ggccgaatta tatccacttt cccgactggc tggggcggtg gttttacgat
      121 gagctgacct atgaagagcg cggcagtgac ggaaaatggc gaaaaccggg caggggcgct
      181 aacgaggcgt ttgacctgct ggtttatgcg gatgcgcttg ctgttctgca tggttatgaa
      241 aagatccgct ggccctccgc accggactgg gcacagcggg aaacgtggct cgtcttcccg
      301 caggagcgtt ctggtgaaac ggtatccccg gaactgacgg ccggggcaga aaaacgccgt
      361 cgccggaaga aaaaactgcg gacggagcgt gcggaagata atccatggat aacatcagga
      421 ggctggttgt gagcacagaa gaagccagag aaatgataca gcggtaccgt gaagcggaaa
      481 tggctgtact ggaggggaag tctgtcacct tcaacgggca gcaactgacg ctggaaagcc
      541 tttctcagat ccgcgccgga cgtcaggagt gggaacgcag gcttgccgcg atggtgagcc
      601 gcaggcgggg aaaaccggga tttaaactgg cgaggtttta atggcaatta ttgatgatgt
      661 gataggcgtg ttttcccccg gatggaaagc agccagactg cgttcaaggg cgttaatcat
      721 ggcctatgag gcggtgaaac cgacccggac acataaagcc cggcgggaaa atcgctctgc
      781 tgatcagctc agtaaatacg gtgcggtttc cctgcgggag caggcccgtt ttctggatat
      841 caatcatgac ctggtgattg gtgtgtttga caagctggaa gagcgggtga ttggtgccag
      901 gggaattatt gtggagcctc agccattacg aaaaaacggg gaaatggcgg cagagctggc
      961 tgcggatatc cgccggttgt gggctgaatg gtccgtgagt ccggatgtga cagggcagta
     1021 tacccgtccc gtgcttgaac gtttactgct gcggacctgg ctgcgggatg gtgaagtgtt
     1081 tg
//
LOCUS       DAZFRN010000395         1064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFRN010000395 DAZFRN010000000
VERSION     DAZFRN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.399"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1064
                     /locus_tag="ACHKUB_005292"
     CDS             <1..>1064
                     /locus_tag="ACHKUB_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Contact-dependent inhibitor A"
                     /protein_id="HFO6658141.1"
                     /translation="GRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATE
                     VNAALHAKDLTVTAGANRITADGRVSALKGEGDVPKVAVDTGALGGMYARRIHLTSTE
                     SGVGVNLGNLYARDGDITLDASGRLTVNNSLATGAVTAKGQGVTLTGDHKAGGNLSVS
                     SRSDIVLSNGTLNSDKDLSLTAGGRITQQNEKLTAGRDVTLAAKNITQDTASQINAAR
                     NIVTVASDTLTTQGQITAGQNLTASATTLTQDGILLAKGHAGLDAGTLNNSGAVQGAS
                     LMLGSTTLSNSGSLLSGSSLTVNTRDFTQSGRTGAKGKVDITASGKLTSTGSLVSDDA
                     LVLKAQDVTQNGVLSGGKGLT"
BASE COUNT          257 a          295 c          330 g          182 t
ORIGIN      
        1 ggcagacctg tgatgaatgc cgacggcagc ctgcaggcgc tggaggtgac tgaaggcagt
       61 atcaccatca atggcgcggg cctggacggc acccggagcg atgccgtatc cattattgcc
      121 cgtgcaacgg aagtgaatgc cgcgcttcat gcgaaggatt taactgtcac tgcaggcgct
      181 aaccggataa ctgcagatgg tcgtgtcagt gcactgaagg gcgaaggtga tgtgccgaaa
      241 gttgccgttg ataccggcgc gctcggtgga atgtacgcca ggcgtattca tctgacctcc
      301 actgaaagtg gtgtcggggt taatcttggt aacctttatg cccgcgatgg cgatatcacc
      361 ctggatgcca gcggcagact gactgtcaac aacagtctcg ccacgggggc cgtcactgca
      421 aaaggtcagg gcgtcacctt aaccggcgac cataaagcgg gaggtaacct gagcgtcagc
      481 agccggagcg atatcgttct cagcaatgga acgcttaaca gcgacaagga cctcagcctg
      541 accgccggcg gcagaatcac tcaacagaat gaaaaactga ctgccggccg ggatgtaacg
      601 cttgccgcga aaaacatcac acaggatacc gccagccaga ttaacgcggc ccgcaatatt
      661 gtgactgtcg ccagtgacac gctgacaaca cagggacaga taaccgccgg gcagaatctc
      721 acggccagcg ccaccacgct gacgcaggac ggaatattgc tggcgaaagg tcatgcggga
      781 ctcgatgccg gtacgctgaa taacagtggc gccgttcagg gggcttccct gatgctcggc
      841 agtacaacgc tcagcaacag tggctccctg ctcagcggca gcagcctgac cgtgaatacc
      901 cgcgacttta cccagagcgg ccgcactggc gcgaagggca aagtggatat cacggccagt
      961 gggaaactga ccagtacagg ttcgctggtg agcgatgatg cactggtgct gaaggcgcag
     1021 gatgtgacac agaacggtgt gctgtccggc ggcaaagggc tgac
//
LOCUS       DAZFRN010000396         1057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFRN010000396 DAZFRN010000000
VERSION     DAZFRN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.400"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            61..>1057
                     /locus_tag="ACHKUB_005293"
     CDS             61..>1057
                     /locus_tag="ACHKUB_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO6658142.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIDNGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDIE
                     LSKGNSALTEKVNRDYFSKPVDDYFLNNHMISYDPSKNQWKNLGTTPFPGTAGSSVIF
                     AEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIF
                     LAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVS"
BASE COUNT          335 a          167 c          249 g          306 t
ORIGIN      
        1 aataaacatg aaaatagtta ccgtgtcggt gttctgatta atttttaaaa ggtggttaca
       61 atgaaagtga aaaactttat atatcttccc ttctgcttgt ttattggaac gtcggtagcc
      121 ggagctttac cggaaattcc tgaaccattt aaatatggtg taggagggat cgataatggt
      181 aaaatatata taggactggg aagtctggga aataactggt atatgataga tacaaaccag
      241 agtgaaaaaa aatggactaa gatagcacaa tggccgacag ttcctagaga gcaggcaaca
      301 gctactatta ttgatggtaa gatctatgta tttggaggaa ttggaaaaga taaaagtgga
      361 gtcataacct tacaaaaaga tgtctattcc tatgatattg ctaaagataa atgggaaaaa
      421 ttgatgaccc gtccccctgt atctcttgcc ggtcatgttt cttttatcca taatggtcat
      481 gcggtatcta ctggtggtgt aaatgagaat atttttaatg gatacttttc agatatcgag
      541 ttatcaaaag ggaattccgc gctgacagag aaagtcaaca gggattattt tagtaaacct
      601 gttgatgatt attttttaaa taatcatatg atttcttatg acccgtcaaa aaaccagtgg
      661 aagaacctgg gaacgactcc atttccggga actgctggtt catcggtgat ttttgcagaa
      721 cagcaaattt atatactggg aggggagaga aaaccgggcc ttcgttctgt tagatcctgg
      781 acgggggagc tttcgcatga tcgaattaaa tggtctgaac ttccaccagt agcttcaccc
      841 gagggggtgt caggagcata tgcctctgtt attgatggaa atatctttct ggccggtggt
      901 gcatatttcc caggggcggc agaaaagtat agcaatggtg aatactggtc tcataaagga
      961 ctggataaag cctattcaaa agagatatat cagctaataa aaaatgactg gaaaaaagtt
     1021 ggttctttgc cggaaggact tgcttatggt gtaagcc
//
LOCUS       DAZFRN010000397         1057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFRN010000397 DAZFRN010000000
VERSION     DAZFRN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.401"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..337)
                     /locus_tag="ACHKUB_005294"
     CDS             complement(<1..337)
                     /locus_tag="ACHKUB_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO6658143.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASNKEGQK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLN"
     gene            complement(972..>1057)
                     /locus_tag="ACHKUB_005295"
     CDS             complement(972..>1057)
                     /locus_tag="ACHKUB_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO6658144.1"
                     /translation="GNIDTKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          305 a          222 c          180 g          350 t
ORIGIN      
        1 gatttagatt tttcagatat ggtcttatga tctttccctt acttaataaa tagcatgtat
       61 aggattcgga aagtccagca tctattttgt acctcattat attagataag gatgtgagaa
      121 attccttgtt atcattcgca gccatatatg ccagtccgag ttcagcctct gcggccttac
      181 aggcaaaata gcttagtgaa cacgcttttt gtccctcttt attactggct gatattagtg
      241 cccataatat tgctttgtct gccaaatcta actctctctg aaattgtctt gcagtaactg
      301 cactcgcaga aatagagaaa aaaatacaat tgttaataac cttgccatat tttttagtcc
      361 gtaactgtta atgtgctatc tggctctgaa aatattaact catttaattt ttgcatgtca
      421 ttagataaac cagttataca accctcggaa cagaatcgag gtcctaaaga cggaccaaaa
      481 gcgtttaaac aactatgtat tgaaaatcca ttgcgattat aagttcctgt attaactgac
      541 ggtactaatc gtatgccgtc aggttttcta ttgcttgctc ccgggtcatt aacattccaa
      601 ctccataggc cttgaggtat tggccatcta tttataaatg tgtacaacgg ggattatttt
      661 tacattgcat attattacca gaagcgactg gaatactaag cacatcataa aaattatcat
      721 cacatggtat atctgaaaca cacgaaagca tacctaaagt tatactgtat aaacaggtgg
      781 ctaatcccaa ggggatcgat tccagaaatc gggttcaacg gatactgata aaaattccac
      841 ctaccttcca gcccaatcgg agcctgagtg ttataccgcc cctgctgcgg ttaatgttac
      901 cgataatagt tatagtacag ccctgattca tcatcatact actgctgcgg cagtctgatg
      961 aactattgct tttacggatt ctccttattc ttccttctat ccattcgctt tagctttttg
     1021 agctcttgaa gtttgctctt tgtatcaata ttaccgt
//
LOCUS       DAZFRN010000398         1056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFRN010000398 DAZFRN010000000
VERSION     DAZFRN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.402"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..671
                     /locus_tag="ACHKUB_005296"
     CDS             <1..671
                     /locus_tag="ACHKUB_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6658145.1"
                     /translation="PERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYRAMTI
                     FDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDG
                     KILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICID
                     KSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTR
                     ICMR"
     gene            839..>1056
                     /locus_tag="ACHKUB_005297"
     CDS             839..>1056
                     /locus_tag="ACHKUB_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658146.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
BASE COUNT          399 a          174 c          183 g          300 t
ORIGIN      
        1 aaccagaacg atctgtaaga ttagctaagc aggaccaagg atataagaat cattatttat
       61 cagatgaaat gttagccgga aagaaagaac tttacgactt cacccctgaa agcatttaca
      121 gggccatgac tatatttgat agattacaaa ataaaagtga tatacagacg ctaaaaacag
      181 aatgttattg ccttctagcg gaatgccata tgtctttggc acttcatgga aaaagtgaac
      241 ttgaacttgc tgctcaaaaa gcattagagc ttttagatta tgtatcagac ataaccactg
      301 tcgatggaaa aattttagct attatgggac tgataactgg tctgtctgga caagcaaaag
      361 tatctcatat cttatttgaa caggctaaga tacactcaac tgatatagcc tctctctact
      421 actatagggc actagtccac tttcataatg aaaaaattga agaggcaagg atttgtatag
      481 acaaatcact acaactcgaa cccagaagac gaaaagcagt tgtgataaaa gaatgtgtag
      541 atatgtatgt gcctaacccg ctcaaaaaca acattaaact ctactataaa gaaactgaga
      601 gtgaaagcca tcgagttata attgacaaca ttttgaaatt aaagcagctg acgagaattt
      661 gtatgcgata aatttttatt gacgcaattt tattatttag atgtatgcaa cttgttattt
      721 aaacaacaga gataaaaata tctaagatat tcaccttatt gcaagatatt taaaatgctc
      781 tagagttaaa agtatgatgt aaagaccatt ggcctggaca attaaaaaag ggagctttat
      841 gactaatcca atcggtatca ataatttatc tcaaagttca aatatagcga atgcaacggg
      901 tgatgaagtc gtatcattag ataaacacat caacaccagt gctactgata cagatcaaat
      961 acaagcattt atagtttcaa catggatggc gccttttcaa aatgatatgt attcagaaga
     1021 taatcctatc tcaccttact ataaaattga atggta
//
LOCUS       DAZFRN010000399         1052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFRN010000399 DAZFRN010000000
VERSION     DAZFRN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.403"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1052
                     /locus_tag="ACHKUB_005298"
     CDS             <1..>1052
                     /locus_tag="ACHKUB_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6658147.1"
                     /translation="HTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVTLPEA
                     GTSTVNLINGSGKPVRVDITAHPAPDRIQVSALPDGVETYGVWGLSLPSLRRRLFRCV
                     SIRENTDGTFAITAVQHVPEKEAIVDNGARFEPLSGSLNSVIPPAVQHLTVEVSASDG
                     QYLALAKWDTPRVVKGVRFSLRLTSGNGENSRLVTSAITADTEHRFRGLPPGEYTLTV
                     RAINSYGQQGEPATTTFRINAPAAPASIELTPGYYQITAVPVLAVYDPTVQYEFWFSE
                     KRITDTAQVETSARYLGTGSQWSVSGPHVKPGKDFWFYVRSVNLVGKSAFVEASGRAS
                     NDAEGYLDFFRGEIGKT"
BASE COUNT          226 a          307 c          335 g          184 t
ORIGIN      
        1 cacacgcccg gtgacatcat tgaaatctgt gataacgact acgccgggac cctgaccggc
       61 ggacgcatcc tgtccatcga tgccgccagc cgtaccctga cgctggaccg tgaggtgaca
      121 ctgccggaag cagggacatc gacggtgaac ctgattaacg gcagcggtaa gccggtgcgc
      181 gtggacatca ctgcacaccc cgccccggac cggatacagg tcagcgccct gccggatggc
      241 gtggagacat acggtgtgtg gggactctcc ctgccgtcac tgcgtcgtcg cctgttccgc
      301 tgtgtttcca tccgggaaaa cacggacggc acctttgcca tcacggcagt gcagcacgta
      361 ccggaaaaag aagccatcgt ggataacggg gcccgctttg agccgctgtc cggttcactg
      421 aacagcgtca tcccgccggc agtgcagcac ctcacggtgg aggtgagtgc ctcagacggc
      481 cagtatctgg cgctggcgaa atgggacacg ccgcgggtgg tgaagggcgt gcgcttcagt
      541 ctgcgcctga ccagcggaaa cggtgaaaac agccgcctgg tgaccagcgc catcactgcc
      601 gacacggagc accgtttcag aggcctgccg cccggggaat acaccctgac ggtcagggcg
      661 ataaacagct acggccagca gggcgaacct gccaccacca cattccggat taatgcgcct
      721 gcagcaccgg ccagcattga gctgacgccg gggtattatc agataacagc agtaccggtg
      781 ctggcggtgt atgacccgac ggtacagtat gaattctggt tctcagaaaa acgcatcacg
      841 gacacggcac aggtggaaac ctctgcccgt tatctgggga caggcagtca gtggagtgtc
      901 tccggcccgc acgttaagcc ggggaaggat ttctggtttt atgtgcgcag cgtcaacctg
      961 gtggggaaat ctgcgtttgt ggaagccagt ggccgggcga gcaatgatgc ggaagggtat
     1021 ctggactttt tcagaggaga aatcgggaag ac
//
LOCUS       DAZFRN010000400         1052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFRN010000400 DAZFRN010000000
VERSION     DAZFRN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.404"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1052
                     /locus_tag="ACHKUB_005299"
     CDS             <1..>1052
                     /locus_tag="ACHKUB_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HFO6658148.1"
                     /translation="HTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVILPEA
                     GTSTVNLINGSGKPVRVDITAHPAPDRIQVSVLPDGVETYGVWGLSLPSLRRRLFRCV
                     SIRENTDGTFAITAVQHVPEKEAIVDNGATFEPLTGSLNSVIPPAVQHLTVEVSASDG
                     QYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLVTSAITADTEHRFSGLPPGEYTLTV
                     RAINSYGQQGEPATTTFRINAPAAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSE
                     AKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRAS
                     NDAEGYLGLFREKIGKL"
BASE COUNT          224 a          305 c          329 g          194 t
ORIGIN      
        1 cacacgcccg gtgacatcat tgaaatctgt gataacgact acgccgggac cctgaccggt
       61 ggacgcatcc tgtccatcga tgccgccagc cgtaccctga cgctggaccg tgaggtgata
      121 ctgccggaag cagggacatc gacggtgaac ctgattaacg gcagcggtaa gccggtgcgc
      181 gtggacatca ctgcacaccc cgcccctgac cggatacagg tcagcgtcct gcctgatggc
      241 gtggagacat acggtgtgtg gggactctcc ctgccgtcac tgcgtcgtcg cctgttccgc
      301 tgtgtttcca tccgggaaaa cacggacggc acctttgcca tcacggcagt gcagcacgta
      361 ccggaaaaag aagccatcgt ggataacggg gccacctttg agccgctcac cggttcactg
      421 aacagcgtca tcccgccggc agtgcagcac ctcacggtgg aggtgagtgc ctcagacggc
      481 cagtatctgg cgctggcgaa atgggacacg ccgcgggtgg tgaagggcgt gcgcttcagt
      541 ctgcgcctga ccagtggcag tggtgaaaac agccgcctgg tgaccagcgc tatcactgcc
      601 gacacggagc accgtttcag tggcctgccg cccggggaat acaccctgac ggtcagggcg
      661 ataaacagct acggccagca gggcgaacct gccaccacca cgttccggat taacgcaccg
      721 gcagcaccgg ccagcattga actgacgccg gggtattatc agataacggc ggtcccgcat
      781 cttacggtgt atgacccgac ggtacaattt gagttctggt tttcggaggc aaaaatcgca
      841 gacacatctc aggtggaaac ctctgcccgt tatctgggga caggcagcca gtggagtgtc
      901 tccggcccgc acattaagcc ggggaaggat ttctggtttt atgtgcgcag cgtcaacctg
      961 gtggggaaat ctgcgtttgt ggaagccagt ggccgggcca gcaatgatgc ggaagggtat
     1021 ctggggctgt ttcgggaaaa gataggaaaa ct
//
LOCUS       DAZFRN010000401         1045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFRN010000401 DAZFRN010000000
VERSION     DAZFRN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.405"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1045
                     /locus_tag="ACHKUB_005300"
     CDS             <1..>1045
                     /locus_tag="ACHKUB_005300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658149.1"
                     /translation="NGWDIKDADGAEVKIVDSAGNVKSGNLYNIARLYVDMNTNKAIT
                     LDSAYNIIGSQKEGTDPAKALIMSDDNDWVYIGGGISPHIGDQNPGSDNNIDMAGGDD
                     ILSSVGAVLDGANINMGDGDDKLYTQDGFASSGTRNIIMGDGNDVISVDNSFGGKNTI
                     SLGEGNNLFIVGNYVNSIAENDITAGSGDDRIEIGTNLDGKNKIDLGDGDNTIQVGGY
                     ITNSHTITGNSGDDIIYVATNIDGSGSFNLGDGNNNFIVGGYIQGKNTIEMGSGDDTV
                     SVSTRIADNVKIQLNAGDDSVYAGGLLNKAIVDLGDGDDVVTLSGISDNGKRNNMEEL
                     VSTNAMLTGGEGN"
BASE COUNT          329 a          144 c          265 g          307 t
ORIGIN      
        1 taaatggctg ggacatcaag gatgcagatg gcgctgaagt aaaaattgtt gattctgctg
       61 gtaacgttaa atcaggaaac ttatataata tcgcgcgttt gtatgtggat atgaatacaa
      121 acaaagctat aacgctggat tcggcttata atataattgg ctctcagaaa gaaggaaccg
      181 atcctgctaa agctcttatc atgtcagatg ataatgactg ggtgtatatt ggtggaggaa
      241 tatctcccca tataggggat cagaatccag gctctgacaa caacattgat atggctggcg
      301 gtgatgatat tctgtcatct gttggtgcag ttttggatgg agccaatatt aatatgggtg
      361 atggagatga taaactatat acgcaggatg gtttcgcttc ttcaggtacc cgaaatatta
      421 ttatgggaga cgggaatgac gttatttctg ttgataattc atttggtgga aaaaatacca
      481 tttctttagg agagggaaac aacttattta ttgttggtaa ctatgtaaat agtattgctg
      541 aaaatgatat tacggctggt agtggtgatg acagaataga aattggtaca aatctggatg
      601 gtaaaaacaa aattgatctt ggggatggtg acaacactat tcaggttgga ggatacataa
      661 ccaactcaca taccattact gggaatagtg gtgatgatat tatctatgtc gctacaaata
      721 tagatggttc tggctcattt aatttaggtg acggtaataa caactttata gtcggaggat
      781 atatccaggg gaaaaacaca attgagatgg gttccggtga tgatacggtt tctgtttcca
      841 ctcgtattgc agataatgtt aaaatccaac ttaatgccgg ggatgacagt gtgtatgcag
      901 gtggattgtt aaacaaggca attgttgacc tcggggatgg ggatgatgtc gtcacattga
      961 gtggaatatc agataatggt aagcgtaata atatggagga gttagtatcc actaatgcaa
     1021 tgcttacagg aggggaagga aacga
//
LOCUS       DAZFRN010000402         1041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFRN010000402 DAZFRN010000000
VERSION     DAZFRN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.406"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            104..>1041
                     /gene="tdcA"
                     /locus_tag="ACHKUB_005301"
     CDS             104..>1041
                     /gene="tdcA"
                     /locus_tag="ACHKUB_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO6658150.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT          291 a          194 c          241 g          315 t
ORIGIN      
        1 catggaaagt attgaaagtt aatttgtgag tggtcgcaca tatcctgttc atttcatttt
       61 gatacacttc atgccgtcaa tgaggtaatt aacgtaggtc gttatgagca ctattcttct
      121 tccgaaaacg cagcacctgg tagtctttca ggaagtcatt agaagtggct ctatcggctc
      181 ggctgcaaaa gaattagggt taactcaacc ggccgtcagt aaaatcatta acgatattga
      241 agattatttt ggtgtggaat tagtggtgcg gaaaaatacc ggtgtaacat taacacctgc
      301 cggtcaattg ttactctccc gttccgaatc cattacccgt gaaatgaaaa atatggttaa
      361 tgagataagc ggtatgtctt ctgaggcggt ggtggaagtc tcatttggtt ttccttcatt
      421 gattggtttt acttttatgt cagggatgat caacaagttc aaagaggtgt tcccgaaagc
      481 gcaggtttct atgtatgaag cgcaactgtc ttcgttctta ccggcaatcc gcgacggtcg
      541 actggatttt gcgattggta cgttaagtgc agaaatgaag cttcaggatt tacatgttga
      601 gccgctgttc gagtccgagt ttgtgctggt agccagtaag tcccgaacat gcaccggcac
      661 caccacgctg gagtcgttga agaacgaaca gtgggtgttg ccacaaacca atatggggta
      721 ctacagcgag cttcttacta cgttacaaag aaatggcatc agtattgaaa acatcgttaa
      781 aaccgactca gtcgtgacaa tttataatct tgttctcaat gctgatttct taactgtaat
      841 tccttgtgat atgacgtcac cttttggttc aaatcaattt attactattc cggttgaaga
      901 aacattacct gtggcacaat atgccgcggt atggtcgaaa aattatcgta ttaaaaaagc
      961 agcatcggtt ttggtggaat tagccaaaga gtattcatct tataatgggt gtagacgaag
     1021 gcaattaata gaagttggtt a
//
LOCUS       DAZFRN010000403         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFRN010000403 DAZFRN010000000
VERSION     DAZFRN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.407"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(1..514)
                     /locus_tag="ACHKUB_005302"
                     /pseudo
     CDS             complement(1..514)
                     /locus_tag="ACHKUB_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(474..593)
                     /locus_tag="ACHKUB_005303"
                     /pseudo
     CDS             complement(474..593)
                     /locus_tag="ACHKUB_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(606..>1024)
                     /locus_tag="ACHKUB_005304"
     CDS             complement(606..>1024)
                     /locus_tag="ACHKUB_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658151.1"
                     /translation="NYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAK
                     SAAIVYKESRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSG
                     TLHEAPSPIIPLNWSKASMTLMLKEMSNLINDEGIK"
BASE COUNT          273 a          223 c          169 g          359 t
ORIGIN      
        1 tccaattaca aaatgaatgg accaatataa tgattttttg tctaacactg gagcgtttcc
       61 gtattggcag actgtaactt tatctaacca aagattcaat tcccctgttc atattcttat
      121 tagtggttga gcagggctca atactccatc aaatacttta cctacgtgca aacgattata
      181 ttttagttca ggatgcttta gatttaacgg ggaactggat aaactgagcc attcccctat
      241 taacctttcc gtttctggca ttaaagccat cccagcgagc tcagatgaaa ttaagggaaa
      301 tatttctcca agcaataccc atccagacca gctgccactc tcttctgcta tcggtaacca
      361 tttagcacta aattcgggca ttaataatgc aacatcttcg ccattttctt tccaattttg
      421 aaaagtggtt tcaaacgtat gagtgtttcg atttactttc ctcagaccga acattaactt
      481 tcatccttct gatcgttttc gaatttgctt tcattttctt gctcctcata aggctgatgt
      541 cgttgtcctt gtcgttcatc ttgctcagtc aatacccaac gttgtgctgt aactcattat
      601 gaaaactatt taattccctc atcattgata aggttggaca tctctttcaa catgagagtc
      661 attgaagctt tcgaccaatt tagcggaatg atagggcttg gcgcttcatg caaagttccc
      721 gacttgtgga tatagttata gctttcggga agtgccattt caggtacact cctgccattt
      781 gcaccaatca tattctcgcc tgttatttgg gccaatgatc tattcataaa ttgcactgag
      841 tcattcaaat actcttcctt tcttgattct ttataaacaa ttgctgctga ttttgcatac
      901 catgagtcaa aaaaccattc tgcttctgtt gatggaatga agctcttttc tcttttcgca
      961 tgtgaatttt gatcggtatc ggttttaata tcattgaacc aaaaatttgc cgactgataa
     1021 ttat
//
LOCUS       DAZFRN010000404         1016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFRN010000404 DAZFRN010000000
VERSION     DAZFRN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.408"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            1..>1016
                     /locus_tag="ACHKUB_005305"
     CDS             1..>1016
                     /locus_tag="ACHKUB_005305"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO6658152.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREG"
BASE COUNT          235 a          269 c          276 g          236 t
ORIGIN      
        1 atggaccaga agctgttaac ggatttccgc tcagaactac tcgattcacg ttttggcgca
       61 aaggccattt ctactatcgc ggagtcaaaa cgatttccgc tgcacgaaat gcgcgatgat
      121 gtcgcattcc agattatcaa tgatgaatta tatcttgatg gcaacgctcg tcagaacctg
      181 gccactttct gccagacctg ggacgacgaa aacgtccata aattgatgga tttgtcgatc
      241 aataaaaact ggatcgacaa agaagaatat ccgcaatccg cagccatcga cctgcgttgc
      301 gtaaatatgg ttgccgatct gtggcatgcg cctgcgccga aaaatggtca ggccgttggc
      361 accaacacca ttggttcttc cgaggcctgt atgcttggcg ggatggcgat gaaatggcgt
      421 tggcgcaagc gtatggaagc tgcaggcaaa ccaacggata aaccaaacct ggtgtgcggt
      481 ccggtacaaa tctgctggca taaattcgcc cgctactggg atgtggagct gcgtgagatc
      541 cctatgcgcc ccggtcagtt gtttatggac ccgaaacgca tgattgaagc ctgtgacgaa
      601 aacaccatcg gcgtggtgcc gactttcggc gtgacctaca ccggtaacta tgagttccca
      661 caaccgctgc acgatgcgct ggataaattc caggccgaca ctggtatcga catcgacatg
      721 cacatcgacg ctgccagcgg tggcttcctg gcaccgttcg tcgccccgga tatcgtctgg
      781 gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag gccataaatt cggtctggct
      841 ccgctgggct gcggctgggt tatctggcgt gacgaagaag cgctgccgca ggaactggtg
      901 ttcaacgttg actacctggg tggtcaaatt ggtacttttg ccatcaactt ctcccgcccg
      961 gcgggtcagg taattgcaca gtactatgaa ttcctgcgcc tcggtcgtga aggcta
//
LOCUS       DAZFRN010000405         1007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFRN010000405 DAZFRN010000000
VERSION     DAZFRN010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.409"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>1007
                     /locus_tag="ACHKUB_005306"
     CDS             <1..>1007
                     /locus_tag="ACHKUB_005306"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO6658153.1"
                     /translation="KQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQIIN
                     DELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVA
                     DLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQ
                     ICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQ
                     PLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGL
                     APLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGRE
                     G"
BASE COUNT          237 a          266 c          271 g          233 t
ORIGIN      
        1 aagcaagtaa cggatttaag gtcggaacta ctcgattcac gttttggtgc gaagtctatt
       61 tccactatcg cagaatcaaa acgttttccg ctgcacgaaa tgcgcgacga tgtcgcattc
      121 cagattatca atgacgaatt atatcttgat ggcaacgctc gtcagaacct ggccactttc
      181 tgccagacct gggacgacga aaatgtccac aaattgatgg atttatccat taacaaaaac
      241 tggatcgaca aagaagaata tccgcaatcc gcagccatcg acctgcgttg cgtaaatatg
      301 gttgccgatc tgtggcatgc gcctgcgccg aaaaatggtc aggccgttgg caccaacacc
      361 attggttctt ccgaggcctg tatgctcggc gggatggcga tgaaatggcg ttggcgcaag
      421 cgtatggaag ctgcaggcaa accaacggat aaaccaaacc tggtgtgcgg tccggtacaa
      481 atctgctggc ataaattcgc ccgctactgg gatgtggagc tgcgtgagat ccctatgcgc
      541 cccggtcagt tgtttatgga cccgaaacgc atgattgaag cctgtgacga aaacaccatc
      601 ggcgtggtgc cgactttcgg cgtgacctac actggtaact atgagttccc acaaccgctg
      661 cacgatgcgc tggataaatt ccaggccgat acaggtatcg acatcgacat gcacatcgac
      721 gctgccagcg gtggcttcct ggcaccgttc gtcgccccgg atatcgtctg ggacttccgc
      781 ctgccgcgtg tgaaatcgat cagtgcttca ggccataaat tcggtctggc tccgctgggc
      841 tgcggctggg ttatctggcg tgacgaagaa gcgctgccgc aggaactggt gttcaacgtt
      901 gactacctgg gtggtcaaat tggtactttc gccatcaact tctcccgccc ggcgggtcag
      961 gtaattgcac agtactatga attcctgcgc ctcggtcgtg aaggcta
//
LOCUS       DAZFRN010000406          989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFRN010000406 DAZFRN010000000
VERSION     DAZFRN010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.410"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..342
                     /locus_tag="ACHKUB_005307"
     CDS             <1..342
                     /locus_tag="ACHKUB_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6658154.1"
                     /translation="EMPDFHREDDWWRNGQNLYLDNLAENGFCRVSPSCAQAGDILLC
                     CFGSSVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRRTHSVWRHRHWSASAFTGI
                     YNDLVAASVCM"
     gene            339..878
                     /locus_tag="ACHKUB_005308"
     CDS             339..878
                     /locus_tag="ACHKUB_005308"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6658155.1"
                     /translation="MNTAAEAIRALSLQVPGFRQKLYEGWYQLRIAGRDVSEQELHSR
                     VCEPLNNGDVIHLVPRTEGAKNGGVLQVVAGAVLAVVGYVFSWTGIGAVIGNLGVAMM
                     VGGITQMLTPRAKTPSATTTDNGKRNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQ
                     DISTRDEGGDGKVVVIGRG"
BASE COUNT          238 a          223 c          304 g          224 t
ORIGIN      
        1 gagatgccgg attttcatcg cgaggatgac tggtggcgca acggccagaa cctgtacctg
       61 gacaatctgg cggaaaacgg cttttgccgg gtgtctccgt cctgtgcaca ggcaggcgat
      121 attctgctgt gctgctttgg ttcatcggtg ccgaatcatg ccgccattta ctgtggcaac
      181 ggtgaactgc ttcaccatat acctgaacaa ctgagtaaac gggagaggta ttctgaaaaa
      241 tggcaacgac gaacgcattc tgtctggcgt caccgccact ggtccgcatc tgccttcacg
      301 gggatttaca acgatttggt cgccgcatca gtctgtatgt gaacacggca gcggaggcca
      361 tccgtgccct gtcgctgcag gtgccgggat tcaggcagaa actgtatgag ggatggtatc
      421 agttacgcat tgcgggcagg gatgtatctg aacaggagct tcattccaga gtatgtgaac
      481 cgttgaataa cggggatgtt atccatctgg tgccccggac ggagggggca aaaaacggcg
      541 gtgttcttca ggtggtggcc ggtgctgttc tggctgttgt gggatatgtg ttcagctgga
      601 ccggtattgg tgctgtcatc ggtaatcttg gggtggccat gatggtgggg ggtatcacgc
      661 agatgctcac ccccagggcg aaaacaccgt ctgccacgac aacagataat ggtaaacgga
      721 acacgtattt ctcctcactg gacaacatga ttgcccaggg taacccgatg ccggtgcctt
      781 acggtgaaat gctggttggt tcacgacgga tatcccagga catcagcacc cgtgatgagg
      841 gcggtgacgg gaaagtggtg gttatcgggc ggggatgaaa ataaaaaaat cccgcagtgt
      901 tgcggagctg cgggaagaga tacgaagatt aactgtaagg aattattatg tcacagcaaa
      961 aaaaacggta acgcagagaa attataagc
//
LOCUS       DAZFRN010000407          984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFRN010000407 DAZFRN010000000
VERSION     DAZFRN010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.411"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..432
                     /locus_tag="ACHKUB_005309"
     CDS             <1..432
                     /locus_tag="ACHKUB_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO6658156.1"
                     /translation="ALWLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTY
                     EERGSDGKWRKPGRGANEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQE
                     RSGETVSPELTAGAEKRRRRKEKLRTERAEDNPWITSGGWL"
     gene            429..641
                     /locus_tag="ACHKUB_005310"
     CDS             429..641
                     /locus_tag="ACHKUB_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658157.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            641..>984
                     /locus_tag="ACHKUB_005311"
     CDS             641..>984
                     /locus_tag="ACHKUB_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6658158.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWA"
BASE COUNT          231 a          230 c          332 g          191 t
ORIGIN      
        1 gcgctgtggc tggttcagac ggatgcgttt aaggaccgtg taaataatgc cctgtggcgt
       61 gacacaccag ggccgaacta tatccacttt cccgactggc tggggcggtg gttttacgat
      121 gagctgacct atgaagagcg cggcagtgac ggaaaatggc gaaaaccggg caggggcgct
      181 aacgaggcgt ttgacctgct ggtttatgcg gatgcgcttg ccgttctgca tggttacgaa
      241 aagatccgct ggccctccgc accggactgg gcacagcggg aaacgtggct cgtcttcccg
      301 caggagcgtt ctggtgaaac ggtatccccg gaactgacgg ccggggcaga aaaacgccgt
      361 cgccggaagg aaaaactgcg cacagagcgt gcggaagata atccatggat aacatcagga
      421 ggctggttgt gagcacagaa gaagccagag aaatgataca gcggtaccgt gaagcggaaa
      481 tggccgtact ggaggggaag tctgtcacct tcaacgggca gcaactgacg ctggaaagcc
      541 tttcccagat ccgcgccgga cgtcaggagt gggaacgcag gcttgccgcg atggtgagcc
      601 gcaggcgggg aaaaccggga tttaaactgg cgaggtttta atggcaatta ttgatgatgt
      661 gataggcgtg ttttcccccg ggtggaaagc agccagactg cgttcaaggg cgttaatcat
      721 ggcctatgag gcggtgaaac cgacccggac acataaagcc cggcgggaaa atcgctctgc
      781 cgatcagctc agtaaatacg gtgcggtttc cctgcgggag caggcccgtt ttctggatat
      841 caatcatgac ctggtgattg gtgtgtttga caagctggaa gagcgggtga ttggtgccag
      901 gggaattatt gtggagcctc agccattacg aaaaaacggg gaaatggcgg cggagctggc
      961 tgcggatatc cgccggttgt gggc
//
LOCUS       DAZFRN010000408          983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFRN010000408 DAZFRN010000000
VERSION     DAZFRN010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.412"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..746)
                     /gene="tssI"
                     /locus_tag="ACHKUB_005312"
     CDS             complement(<1..746)
                     /gene="tssI"
                     /locus_tag="ACHKUB_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO6658159.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQ"
BASE COUNT          247 a          245 c          263 g          228 t
ORIGIN      
        1 tcctggtgct ggccttcctg ttcaaaacgt ccggcccagc cggggcgttt aaaggtgtag
       61 tctttggtca ccacggaaga agggcggatt tgtgcgctgt agcggaactg gctgatgcag
      121 agggtgctca cctcggtacg ggtgttcggg ttccatggga tttcaaaaga ttcgggcaga
      181 tggcggactg tgtcgcacag caccaggctc tggtcggtac ttttgtaagc atgctcctca
      241 taaaagaaga tgccttcctc cgccgccatc cggcacagga aatcgtaatc agtctcaccg
      301 tactggacac aaaactcacg ggaaggatgc ggctcgctga acagcggact ccattcggtc
      361 accccgtttt cctgcaacat cgtgccgagg atgcttttga tgtcctcgtt ctggaagata
      421 cggaagttct ggcgcagacc ggcacgccac agcggcgggt gcaccttcat gctgtacagc
      481 atctggtttt tgtcgttctc cccgagttca aaccaggtca ccacgccttt cacccggcgc
      541 tgtacttcat cgccctgcca tatcgtcagg taggccattt tgtccagcac ctgcgcaaat
      601 tcaagggaga gaaactgctg gctgaccagg gagagatcga gggaaaagag cgaagagagt
      661 gactggttca gatgaaagga aaccaccgca aaagcatccg gcggcaggcc gtccacttcc
      721 agtgtgaaac gtaatccggt tgacatagca acctccagta aaatgaataa tgatccgtac
      781 cgtacaactg cggataatgt gataagcggg acgacttcag acgttattag ggaaggaaca
      841 gaaaatgcta tggttatgtg agaattttgt ttaaatatta ctttacagaa acgggtgtct
      901 gttcagtcaa aaaaataatc acaagctcaa cctcttgttt cttggcgtta ttcaggacag
      961 gtacgcatat cacaacgagt aac
//
LOCUS       DAZFRN010000409          972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFRN010000409 DAZFRN010000000
VERSION     DAZFRN010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.413"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..299
                     /locus_tag="ACHKUB_005313"
     CDS             <1..299
                     /locus_tag="ACHKUB_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO6658160.1"
                     /translation="PVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSFSV
                     VVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            296..>972
                     /locus_tag="ACHKUB_005314"
     CDS             296..>972
                     /locus_tag="ACHKUB_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6658161.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCG"
BASE COUNT          221 a          238 c          293 g          220 t
ORIGIN      
        1 tgcctgtaga taacgaggaa atggatcctc atgcgcgtaa agcatggtac cagtcagaac
       61 gtgaccgact gaaatttgag caggaaactg gtcagcttgt gccagtcagt gatgtcaggc
      121 ggtccttttc tgtcgtggtg aaagcgatag ttcaggtact ggaaacctgg cctgaccggc
      181 tggagaggga cagggggtgg accgcatcac aactgaatga agtacagatt gtggttgatg
      241 agatccgcga cacactggaa aaggcagtca ttgactgctg tgatgaggcc gatatgtgaa
      301 tcaggtgaac gagagccata gccgcgcatc cgatatctgg cgcgaagtgg cctcgctgtt
      361 tcgcccacct ggccggttac cagtagcgga agccatcagg cgttatatgc gggtaccacg
      421 gggagccaat acttccggtc cgtgggagtc atcgctgacg ccctatatga tagaccccat
      481 taatacatta tcagcccgtg aatatgacgc ggtggtgttt gtcggacctg cgcgaaccgg
      541 gaaaaccgaa gggctgattg atggctggat tgtgtacggc atcatctgtg atccggcgga
      601 tatgctggtg gtgcagatga ctgagacgaa ggcgcgtgag cattccagaa cgcgtctttc
      661 caggacgttt cgccacagtc cggaggtcag caagcgcctc agtccttccc gtaatgacaa
      721 caacgtccac gataaaatgt ttcttgacgg ctccttcctg aagattggct ggccgtcgat
      781 cactgtattt tcctcttcgg attaccgtcg tgtggcgctg acggattatg accgtttccc
      841 tgaaaacgtg gacggggaag gggatgcctt cacgctggcc tcaaagcgta ccaccacctt
      901 tatgtcctcg gggatgaccc tggtcgagag ttcaccgggg cgggatatca ctgacaccaa
      961 atggcgttgt gg
//
LOCUS       DAZFRN010000410          966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFRN010000410 DAZFRN010000000
VERSION     DAZFRN010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.414"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(181..852)
                     /gene="queE"
                     /locus_tag="ACHKUB_005315"
     CDS             complement(181..852)
                     /gene="queE"
                     /locus_tag="ACHKUB_005315"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO6658162.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          258 a          239 c          209 g          260 t
ORIGIN      
        1 accaaacttt accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag
       61 agaatgctaa tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca
      121 atataaatga gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa
      181 tcaggcaata tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca
      241 ggtttcaatg cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac
      301 tcgcggttta tcatcggtca gcgttgccag tagttcatcc agtgcttcaa tatcacgtac
      361 gcgccccact ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacttcata
      421 gccgccgcgc atgttcagct ttggtgatac ggtaacccag gtattcggtg tgcagcgtac
      481 ttcatgagtt ccactggttt cgatctggca gctaaaaccg ttcttttcga gcaggtcagt
      541 cagtggcagc aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt
      601 atatccctgc cgaccaatga cagccagcaa atcttcactg ctcgccgccc cccacttatc
      661 actctctttg gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc
      721 ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa
      781 aatggcgggg acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat
      841 cgggtactgc atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgccg
      901 caacaatcat gtcttattaa cattctgtta catgcagatt taaagaaaaa tgcgaaacat
      961 atcgtt
//
LOCUS       DAZFRN010000411          954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFRN010000411 DAZFRN010000000
VERSION     DAZFRN010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.415"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..837)
                     /locus_tag="ACHKUB_005316"
     CDS             complement(<1..837)
                     /locus_tag="ACHKUB_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO6658163.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQIRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESLWLVRGGKATQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSELVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQRTASLSSPDTPRPL
                     SAS"
BASE COUNT          189 a          314 c          271 g          180 t
ORIGIN      
        1 tgaggctgag agagggcggg gtgtgtcagg ggaagataag gaagcggtgc gctgtttacg
       61 ggcctcttcc gcacgctgcg cctgcgtggt cagcaccaga cggaactccc gcccggcacc
      121 gtccgtcacg ccggtgattt ccccggccag gtcaccggcg gcctcacgcc ggtacgtcag
      181 cgtccgcccg aagcggtctg ccagcccggt aagcacccgg tacggcggca gcggcgctgg
      241 cagtacgtcc tcagcacccg gcaccagctc tgaccacccc agtatccacc acggcccctg
      301 tgcgctgttg gtcgccaggt aaagatgcgg gcttaaccgg atatccggcg gcagcgacgc
      361 ccacagccgt gccagcgtat ggccgtccgg ctgcgttgcc ttaccgccac gcaccagcca
      421 cagggactcg ctgcggctgt acaccgcctc acccggcagc agcggctcaa agtgaatgct
      481 ccgtccgccg ttgtcgttga gtaccagtgc atcatcgcgt atctgtaagc ggatatcaga
      541 aggcgctttc cagccggggc caaaaatccc caccggtgca ggcgtcttcg tccggtagct
      601 gctgtaggtg cgggagagaa tgaacggcag cgggccgggc agcgcaaggt ccgtctcgcc
      661 gggcagcacc ttcgccccca gcagcggatt taccgggttg cccgaagtca tcccgcccgg
      721 acacaccgag cacgccacgc cagttggcgc gccaattctt acacctgccg aaccctggac
      781 aatgggaccg ccatactgag tcatatctcc ctgacgcgcc gctggttttc cgctcatata
      841 aagtgccctt ttagttattg ttcatatccc tgtgttagca acaaaaaaaa taaaatgaca
      901 cacaagacca gctaatgtat ttatatattt ttcacataaa cttatttgtt ctca
//
LOCUS       DAZFRN010000412          952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFRN010000412 DAZFRN010000000
VERSION     DAZFRN010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.416"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>952
                     /locus_tag="ACHKUB_005317"
     CDS             <1..>952
                     /locus_tag="ACHKUB_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO6658164.1"
                     /translation="SVSATLPDGANKGDKVEVTFTDEEGNEQKVTLEKGDGNWSSDKP
                     ELIPDSTDNKVTVPADKVKNNTEVTATAKDPGGNESAPVTVTSKTDGVSDAPVLTIPE
                     VADSVANAAELKDGLQAEVKLPAGTVEGAVITLTVTHPDKTTRTETHTVSKDEAADGT
                     VSMVVPKGSVVDGQNSVSVSLTQGSNPAKAGNKVEFVVDGQVPGDTNGDGVADVTPAV
                     AIPEATGGVNAKELKDGVQAEVTVPAGSAEGDTVTLTVTKPDGKTETVSHKLTAEEVK
                     DGKANVGIPADKVTQDGEYTVKAEITDPAGNTSGQGKATQF"
BASE COUNT          278 a          194 c          305 g          175 t
ORIGIN      
        1 ttctgtatca gcgactctgc cagatggtgc gaacaaaggt gacaaggtgg aagtgacctt
       61 tacggatgaa gagggtaatg agcagaaagt cacgctggag aaaggggatg gcaactggag
      121 ttccgacaag cctgaactga tcccggacag caccgataat aaagtgacag ttccggcaga
      181 caaagtgaag aacaacacag aggtgacggc aacagcgaaa gatccgggtg gtaatgaaag
      241 tgctccggtc actgtgacgt caaaaactga cggtgtgtcg gatgctcctg tcctgaccat
      301 tccagaagtg gctgatagtg tggccaatgc ggctgagctg aaagatggtt tacaggcaga
      361 agtcaagttg ccggcaggaa cagtggaagg tgctgtcatt acactgacag tgactcatcc
      421 ggataaaacc accagaaccg agacgcatac tgttagcaaa gatgaggctg cagacggtac
      481 tgtgtcaatg gttgttccga agggaagtgt tgttgacggt cagaacagtg tgagtgtcag
      541 cctgactcag ggcagtaacc cggctaaagc ggggaataaa gtggagtttg tggttgacgg
      601 gcaggttccg ggtgacacca atggtgatgg tgtggcggat gtcacaccgg cagtggccat
      661 tccggaagcg acgggcggag tgaatgccaa agaactgaag gatggcgtac aggcagaagt
      721 gacggttccg gcaggcagtg ctgaaggtga tactgtgacg ctgacggtca ccaaaccgga
      781 cgggaaaaca gaaacggtca gtcacaaact tactgccgaa gaagtaaaag atggtaaagc
      841 gaatgtgggc attcctgcgg ataaagtgac gcaggatggg gaatacacag ttaaggctga
      901 aatcacagac ccggcaggca acaccagtgg tcagggtaaa gctacccagt tt
//
LOCUS       DAZFRN010000413          949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFRN010000413 DAZFRN010000000
VERSION     DAZFRN010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.417"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..276
                     /locus_tag="ACHKUB_005318"
     CDS             <1..276
                     /locus_tag="ACHKUB_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO6658165.1"
                     /translation="DPHARKAWYQSERDRLKFEQETGLLVSVSDVRRSFSVVVKAIVQ
                     VLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            273..>949
                     /locus_tag="ACHKUB_005319"
     CDS             273..>949
                     /locus_tag="ACHKUB_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6658166.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCG"
BASE COUNT          213 a          238 c          282 g          216 t
ORIGIN      
        1 gatcctcatg cgcgtaaagc atggtaccag tcagaacgtg accgactgaa atttgagcag
       61 gaaaccggtc tgctcgtgtc agtcagtgat gtcaggcggt ccttttctgt cgtggtgaaa
      121 gcgatagttc aggtactgga aacctggcct gaccggctgg agagggacag ggggtggacc
      181 gcatcacaac tgaatgaagt acagattgtg gttgatgaga tccgcgacac actggaaaag
      241 gcagtcattg actgttgtga tgaggccgat atgtgaatca ggtgaacgag agccatagcc
      301 gcgcatccga tatctggcgc gaagtggcct cgctgtttcg cccacctggc cggttaccag
      361 tagcggaagc catcaggcgt tatatgcggg taccacgggg agccaatact tccggtccgt
      421 gggagtcatc gctgacgccc tatatgattg accccattaa tacattatca gcccgtgaat
      481 atgacgcggt ggtgtttgtg ggacctgcgc gaaccgggaa aaccgaaggg ctgattgatg
      541 gctggattgt gtacggcatc atctgtgatc cggcggatat gctggtggtg cagatgaccg
      601 agacaaaggc gcgtgagcat tccagaacgc gtctttccag gacgtttcgc cacagtccgg
      661 aggtcagtaa gcgcctcagc ccttcccgta atgacaacaa cgtccacgac aaaatgtttc
      721 ttgacggctc attcctgaaa attggctggc cgtcgatcac tgtattttcc tcttcggatt
      781 accgtcgtgt ggcgctgacg gattatgacc gttttcctga gaacgtcgac ggtgaagggg
      841 atgccttcac cctggcctca aagcgtacca ccacctttat gtcctcgggg atgaccctgg
      901 tcgaaagttc gcccgggcgg gatatcactg acaccaaatg gcgctgtgg
//
LOCUS       DAZFRN010000414          943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFRN010000414 DAZFRN010000000
VERSION     DAZFRN010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.418"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(718..>943)
                     /locus_tag="ACHKUB_005320"
     CDS             complement(718..>943)
                     /locus_tag="ACHKUB_005320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658167.1"
                     /translation="LMQLILSIYHVICSVTSKINIKNSAKFNIRSTSRLTYDIISQLH
                     LPQNGYFMERSNFLVAIIMLILRSLAIIPG"
BASE COUNT          291 a          166 c          188 g          298 t
ORIGIN      
        1 acttactcaa tccattttac ccagagtcat aaaatagaat gtctgttgat ctcattttca
       61 tattcctttc agactgaagt gcgttttcat cacccgatga gaaagaactg aagtgatgtt
      121 tgccatagat tgcgagatgc cctgtctcat tgactatctt ggtgcacctt tatacctgtt
      181 ataccagatc aaaaatcacg caatccatac aactaaacca gatttgcaat tcgtgtcaca
      241 aaatatgtcg atatttttct aagaagaaga tgccatgtga agccagacga acacttgcgg
      301 aggccttcac aaaccaaaga ttgttgttta attatctgtt ttataagtaa tttatttaaa
      361 gtataaacaa ggaaagattt gcaagttaac tcagtgagcg tggtgaaaat cagtgtttac
      421 ccgccatcag gctgagcata attctcatca tgaaatatgt ttcctggttt gtggcttgta
      481 actggtcact tctgaagtcg atctggagag gcttgttgat gttggtgttt tcaggatgat
      541 gtttcactta gtttgtttgc cgtatcgccc ggcgaatggc tgtgattgag gaaggtttaa
      601 gtcgtagtga ccaaagctat atttaccaac gaatgtagat gaaaaaatca tctcctgcgt
      661 tccccatatc tccaggataa aaaggaatgt aacaatctca tggcgtaagc tgacgaatca
      721 gccaggaata atcgctaggg acctaagaat tagcatgata atagccacta agaaattact
      781 gcgctccatg aaatagccat tttgtggcaa atggagttga ctaataatgt catatgtgag
      841 acggctagtt gaacgaatat taaattttgc tgaatttttt atgttgattt tacttgttac
      901 agaacatatc acatgatata tagataagat tagttgcatt aat
//
LOCUS       DAZFRN010000415          937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFRN010000415 DAZFRN010000000
VERSION     DAZFRN010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.419"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>937
                     /locus_tag="ACHKUB_005321"
     CDS             <1..>937
                     /locus_tag="ACHKUB_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO6658168.1"
                     /translation="FRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLT
                     SKYAEHTPLYRQSEIYGRQGVELRRSLLSGWVDACCRLLSPLEEALHGYVMTDGKLHA
                     DDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTHLACF
                     SGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQLYA
                     IEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPA
                     LTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGS"
BASE COUNT          202 a          252 c          297 g          186 t
ORIGIN      
        1 ttccgggtta tccggacggt acgggaaaaa catgcctgta ctcagtgcga tgccatcgtg
       61 caggcacctg caccttcgcg gcccatcgag cggggtatcg ccggaccggg gctgctggcc
      121 cgcgtgctga cctcgaagta tgcagagcac accccgctgt atcgccagtc agaaatatac
      181 ggccggcaag gtgtggagct gaggcgttca ctgctgtcgg gctgggtgga tgcatgctgc
      241 cggctgctgt ctccgctgga agaggcgctt catggctatg tcatgactga cggcaaactc
      301 catgccgatg ataccccggt ccaggtactg ctgccgggta ataagaagac gaagaccggg
      361 cggttgtggg cgtatgttcg tgatgaccgc aatgccgggt cagcgttggc acctgcagtg
      421 tggttcgctt acagcccgga cagaaaaggc atccatccgc agactcatct tgcctgcttc
      481 agcggtgtgc tgcaagcgga tgcgtacgcc gggttcaacg agctttatcg caatggtggg
      541 ataacggaag ctgcctgctg ggctcatgcc cgccgaaaga tccacgatgt gcacgtccgc
      601 atcccgtcag cactgacgga agaagccctg gagcagatcg gtcagttgta cgccatagag
      661 gcggatataa ggggaatgcc ggcagagcag cggcttgctg aacgtcagcg aaaaacgaaa
      721 ccgttgttga aatccctgga aagctggttg cgtgaaaaga tgaagaccct gtcgcgacac
      781 tcagagttgg cgaaggcgtt cgcgtacgca cttaaccagt ggccggcact gacgtactat
      841 gcgaacgatg gctgggtgga aatcgacaac aacatcgctg aaaatgccct gcgggcggtc
      901 agtctgggtc gtaaaaactt cctgttcttc ggctctg
//
LOCUS       DAZFRN010000416          935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFRN010000416 DAZFRN010000000
VERSION     DAZFRN010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.420"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(95..325)
                     /gene="ynfN"
                     /locus_tag="ACHKUB_005322"
     CDS             complement(95..325)
                     /gene="ynfN"
                     /locus_tag="ACHKUB_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO6658169.1"
                     /translation="MKKIIVFFNSEPAMVVPVMTGVNTIMREYPNGETTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAMRLLN"
     gene            complement(357..395)
                     /locus_tag="ACHKUB_005323"
                     /pseudo
     CDS             complement(357..395)
                     /locus_tag="ACHKUB_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(398..586)
                     /gene="ynfQ"
                     /locus_tag="ACHKUB_005324"
     CDS             complement(398..586)
                     /gene="ynfQ"
                     /locus_tag="ACHKUB_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO6658170.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSHLSLDLRK"
     gene            complement(597..809)
                     /gene="cspI"
                     /locus_tag="ACHKUB_005325"
     CDS             complement(597..809)
                     /gene="cspI"
                     /locus_tag="ACHKUB_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO6658171.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVTL"
BASE COUNT          314 a          184 c          157 g          280 t
ORIGIN      
        1 gatgaaacat cgcaataatc aacatacgac ggtctaaatt tcacacaaac agataaagat
       61 gattatcatt tattatcaat gcattagaat caaatcaatt caagagtctc attgctgctt
      121 ccagaatttc ttctgaagtt acatgtcgat ccgctgctac ataaatgact ttatgatctc
      181 cggtcagaga tggaaaccct gcagccatta cagtgaggtg tgttgtttcg ccatttggat
      241 attcacgcat gatggtgtta actccggtca tcactggcac taccattgct ggttcagagt
      301 taaaaaaaac tattattttt ttcataatgt taccgtaata tgtgagtatc catcgactag
      361 acactaagca aaaaagctcc cgaaggagcc ttcattttca ctttcttaaa tctaacgaca
      421 gatggctaac atttaagtat tgtgaaatat tatcaaatgt aatcatcatt gatttacaaa
      481 agatacattt tgccccgaaa ggatttctgt cagaaacatc aaaagatgat gttctatact
      541 gagaaccatg gcaacacggg catctaaagt gaatatggtt tgtaatattt tctacctcaa
      601 agtgtcacta catgaacagc tgcagggcct ttaggtccgt tctcaatacc aaattcgact
      661 tcctgattct cagttaacgt tttgaaatcg ttgctctgaa tagctgagaa atgcacaaac
      721 acatctttgc taccatcttt cggcgtgatg aaaccaaaac ctttttcagg gttaaaccat
      781 ttcactaaac cagtcatttt gttagacatc attattacct tttgaagaag ttagcccttg
      841 ggcagaatgg tccgaaaaaa atatcagaga gaaaaaccaa caaggaaatc tcaagaggta
      901 caaataataa aattataaca atgactgctt cagat
//
LOCUS       DAZFRN010000417          905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFRN010000417 DAZFRN010000000
VERSION     DAZFRN010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.421"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..782
                     /gene="gadX"
                     /locus_tag="ACHKUB_005326"
     CDS             <1..782
                     /gene="gadX"
                     /locus_tag="ACHKUB_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO6658172.1"
                     /translation="KYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTL
                     SLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRAL
                     IFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLL
                     KKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYG
                     MTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          260 a          173 c          205 g          267 t
ORIGIN      
        1 ataaatatat tctcaccatg gttaatggtg aatatcgcta ttttaatggc ggtgacctgg
       61 tttttgcgga tgcaagccaa attcgagtag ataagtgtgt tgaaaatttt gtattagtat
      121 caagggatac gctttcatta tttctgccga tgctcaagga ggaggcatta aatcttcatg
      181 cacataaaaa aatttcttca ttactcgttc atcactgtag cagagatatt cctgtttttc
      241 aggaagttgc gcaactatcg cagaataaga atcttcgcta tgcagaaatg ctacgtaaaa
      301 gagcattaat ctttgcgttg ttatctgttt ttcttgagga tgagcacttt ataccgctgc
      361 ttttgaacgt tttgcaaccg aacatgcgaa cacgagtttg tacggttatc aataataata
      421 tcgcccatga gtggacacta gctcgaatcg ccagcgagct gttgatgagt ccaagcctgt
      481 taaagaaaaa attgcgcgaa gaagagacat catattcaca gttgcttact gagtgtagaa
      541 tgcaacgtgc tttgcaactt attgttatac atggcttttc aattaagcga gtcgcagtgt
      601 cctgtggata tcacagcgtg tcgtatttca tttacgtctt tcgaaattat tatgggatga
      661 cgcccacaga gtatcaggag cgatcggcgc agggattgcc gaaccgtgac tcggcggcaa
      721 gtattgttgc gcaaggaaat ttttacggca ctgaccgttc tgcggaagga ataagattat
      781 agaattttac tcagacataa aaaaaacccg gcatagggga ccgggaagag gatagtctgc
      841 cgtctccaga ctaataaacc gttataacac tccctgttgg cacgggaaac tttgtgctct
      901 cagta
//
LOCUS       DAZFRN010000418          902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFRN010000418 DAZFRN010000000
VERSION     DAZFRN010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.422"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..511
                     /locus_tag="ACHKUB_005327"
     CDS             <1..511
                     /locus_tag="ACHKUB_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658173.1"
                     /translation="FEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGNDPHHVRLS
                     PHRLIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSIFEGDKTLPVVDYMVVLN
                     HGTTVDGEEKNIISAVFLSNMDGNVQNVPVRLKLKSQLRTGYAERYQAIASQYSSRIG
                     VDYVSMMLP"
     gene            complement(583..>902)
                     /locus_tag="ACHKUB_005328"
     CDS             complement(583..>902)
                     /locus_tag="ACHKUB_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658174.1"
                     /translation="VLMNPSADNIAKLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQG
                     DIGPTIGTATLAVYLVYAFHFLSENPEALNLKLLTNSGFIYSTTLLLFSLECMKLRRL
                     KQK"
BASE COUNT          282 a          143 c          174 g          303 t
ORIGIN      
        1 ttttgaagat ggtcatattt ctactggcac tattttcttt gatacagagt cgaacaaatg
       61 gtatttatgt gtatctgcgg catgtgattt ggttcctact cagggtaacg accctcacca
      121 tgtaagatta agtccgcaca ggctcattaa agttctggag ctttttaacg ccagtcagag
      181 taaagcattg ccatttgctg aacattcgaa atatatatat gtaatgcata aaaatcaaag
      241 aaaatatctc tctattttcg aaggggataa aacgcttcct gttgttgatt atatggtggt
      301 gttgaatcat ggaacaacag ttgatggcga agaaaaaaat attatttctg ccgtgttttt
      361 aagtaatatg gatggcaacg tgcaaaatgt tcctgtccga ctcaaactta aatctcaact
      421 gagaactggt tatgcagaaa gatatcaggc tatagcgtct cagtatagct caaggattgg
      481 tgtggattat gtatcaatga tgctaccata attattatat tttaggcgtg gtgatttttt
      541 ttcgccatgc ctattttata tttatcatca caatgatgtt gtttattttt gtttaagtct
      601 tctaagcttc atgcattcta atgagaataa taatagagtt gtgctgtata taaagccaga
      661 gttagttaat aactttaaat ttaatgcttc aggattttca ctgagaaagt gaaaggcata
      721 aactaagtat acagctaaag tagccgttcc aattgttggt ccaatatcgc cttgatcatg
      781 aggttcaagg tttacattga acttgaagat tagccattca aatccccaag taaaaaaaac
      841 aactaatatc atggctgcga ataatttcgc gatgttatct gctgatggat tcatcagaac
      901 ag
//
LOCUS       DAZFRN010000419          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFRN010000419 DAZFRN010000000
VERSION     DAZFRN010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.423"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..475)
                     /gene="sctT"
                     /locus_tag="ACHKUB_005329"
     CDS             complement(<1..475)
                     /gene="sctT"
                     /locus_tag="ACHKUB_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO6658175.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDASELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWP"
     gene            complement(477..736)
                     /locus_tag="ACHKUB_005330"
                     /pseudo
     CDS             complement(477..736)
                     /locus_tag="ACHKUB_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
     gene            complement(746..>900)
                     /locus_tag="ACHKUB_005331"
     CDS             complement(746..>900)
                     /locus_tag="ACHKUB_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6658176.1"
                     /translation="SSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYF
                     DLSINP"
BASE COUNT          313 a          203 c          153 g          231 t
ORIGIN      
        1 aaggccataa attataactt tgccaaagag tttccagaat aaagttcatt ccacctttag
       61 taagataaac cgcagcagag aaaaggttaa ataatcttgc taattccgag gcatcgacac
      121 ctgtagccgg atccaaagta ctacttaatg ttgcaccgcg ttggttatcg ataaagctac
      181 ctatagcaag gaacatccaa aacggggatg ctaataagca tgcaataaac agccctatga
      241 taatttctct ggcagcgaac aaagcaatat cgatttcatt taagttcaat aattcatagt
      301 ggtaatgctg aactaatgca catgatacca ctattattat tgggtgacga actactgttg
      361 gtatattccc actggcgaaa aatggtaaca aatagaaagt tggtgccaat cggcaaaaac
      421 cgagcgctgt agcagccagc agactatgca actgatagag tatcgcctcc cccatgctat
      481 cctctcgcga atgctaaatt cagcatttcg ataccaaatg aatataattt ttcaccatac
      541 cacccgctca ttaaaaaaaa gcaaatactt acgcacaata atttcacgcc aaatggcaac
      601 gtctgttctt gcaactgcgt taccgtttga aaaagccaac caataaacca acaaatgtcg
      661 caactgcaat aggtcctgca gacataacta aaatcagata cagcgcccta ttccccgcaa
      721 aaacaatatc gtccatattt tttcctcagg gattaatgga taggtcaaaa tattgtaaaa
      781 tgagcccttt tgataacatt gtccaaccgt ccatcgcgac aaacaaaatt aattttatcg
      841 gtgtagatat ggttaccgga ctcatcatca tcatacctaa cgttaacaga acgctggata
//
LOCUS       DAZFRN010000420          896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFRN010000420 DAZFRN010000000
VERSION     DAZFRN010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.424"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            4..765
                     /gene="ydeO"
                     /locus_tag="ACHKUB_005332"
     CDS             4..765
                     /gene="ydeO"
                     /locus_tag="ACHKUB_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO6658177.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          271 a          178 c          176 g          271 t
ORIGIN      
        1 aaaatgtcgc tcgtttgttc tgttatattt attcatcatg ccttcaacgc taacatttta
       61 gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc tgttcgtacg
      121 cattttgaac cttatgagcg gcattttaaa gagatcggat ttactgaaaa taccattaaa
      181 aaatatctac aatgcactaa catccagaca gtgacggtgc ctgttcctgc gaagttttta
      241 cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct caactcggaa
      301 gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc tatttttgcc
      361 gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc cggaaaagtg
      421 agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat ttgtgactgc
      481 ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac gacattctca
      541 cagattctat tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca
      601 gtcaataaaa ttgccgaaca atgtggttat gccagtacat cttattttat ttatgcgttc
      661 cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg tcaaagtcac
      721 acgggtatga atacgggcaa cacgatgagt gctttagcta tttgattatt tgctaacgag
      781 tagtcaacca cgcacgctac gtaagaatta tatggggcag ccattccctg ccccgcgttg
      841 tttttaggcg atatatttat tgaaataaat aagtgacatc catcacatat ttatgc
//
LOCUS       DAZFRN010000421          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFRN010000421 DAZFRN010000000
VERSION     DAZFRN010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.425"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>883
                     /locus_tag="ACHKUB_005333"
     rRNA            <1..>883
                     /locus_tag="ACHKUB_005333"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          208 a          201 c          279 g          195 t
ORIGIN      
        1 agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat tccttgtcgg
       61 gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac ccgagactca
      121 gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa agaccccgtg
      181 aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata ggtgggaggc
      241 tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc tttaatgttt
      301 gatgttctaa cgtggacccg taatccgggt tgcggacagt gtctggtggg tagtttgact
      361 ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat cctggtcgga
      421 catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac ggcgcgagca
      481 ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat cgctcaacgg
      541 ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat cgacggcggt
      601 gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc caagggtatg
      661 gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag acagttcggt
      721 ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg agaggaccgg
      781 agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg tagctaaatg
      841 cggaagagat aagtgctgaa agcatctaag cacgaaactt gcc
//
LOCUS       DAZFRN010000422          880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFRN010000422 DAZFRN010000000
VERSION     DAZFRN010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.426"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>880
                     /gene="rfaL"
                     /locus_tag="ACHKUB_005334"
     CDS             <1..>880
                     /gene="rfaL"
                     /locus_tag="ACHKUB_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO6658178.1"
                     /translation="LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSP
                     DAGASMKEIFKAIIENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELIT
                     YYKDYQQGIMPFADYRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLIL
                     GTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQ
                     TNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHN
                     FALFIWFGTGLLGLVSL"
BASE COUNT          256 a          158 c          163 g          303 t
ORIGIN      
        1 tcttatgaca ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact
       61 atttaaaaca tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact
      121 tcttcaatcc cctgatgcag gtgctagcat gaaggaaatt ttcaaagcta ttattgagaa
      181 tactttacta tgcacaatag caataccgat catattgaga gacgagaaaa gagaagatgt
      241 cgaaaaaatc gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt
      301 gattacctat tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg
      361 tagcatttct gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa
      421 atcagcaaaa taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt
      481 aggaacttta tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct
      541 gatgtttaaa caatggaagt tactattagt aggagtaatg gttgccatca ttgcattgtc
      601 ggttattttc acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa
      661 tagttcttat cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa
      721 tcctgttatt ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt
      781 cattgattat ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt
      841 catctggttt ggcactggtt tattagggct ggtaagtctt
//
LOCUS       DAZFRN010000423          874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFRN010000423 DAZFRN010000000
VERSION     DAZFRN010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.427"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..219
                     /locus_tag="ACHKUB_005335"
     CDS             <1..219
                     /locus_tag="ACHKUB_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6658179.1"
                     /translation="QMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEM
                     LVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          277 a          163 c          239 g          195 t
ORIGIN      
        1 cagatgctgg ccccgaaggc aaagacgccg gagtataaaa gtacggataa cggtaaacag
       61 aacacgtatt tttcgtcact ggacaacatg attgcccagg gtaacccgat gccggtgcct
      121 tacggtgaaa tgctggttgg ttcacgacgg atatcccagg atatcagcac ccgtgatgag
      181 ggcggagacg ggaaagtggt ggttatcgga cggggataaa aataaaaaaa tcccgcagag
      241 ttagcggagc tgcgggaaga gatacgaaga ttaactgtaa ggagttatta ttatgtcaca
      301 gcaaaaaaaa cggtaacgca gagaaattat aagcgccact gggattttgt gaaaatgtga
      361 agaatttcag aaattttttg ctgcagaggt gcagggagct tgccgggtat ctgtggcaga
      421 agacagaaaa catcatgccg gggttaaggg ataaaaaaat cccgcagagt ccggagactg
      481 cgggacagaa ccgatgaaga ttaacgttca aggagttttg tttttattga cccgaaaaaa
      541 ctgtaacgcc ccggaatgat atctgccacg ggaacgtaca gaaaatgtga agaaattcag
      601 actttttctg tcgcaggttt acagaacgga tgaccgccag ggtaccagtg gcgtaaatga
      661 gcaggatgtt gtggtgtggc tgaagcaaaa aaatctcccg taatatcgcc ggatactgac
      721 gggagacgaa tagtcacctt aaggaggtct gataattatt gcttttattg agcaaaaaaa
      781 ctgtaacgct gcgtcattat agtggctaca ggcaattgcc ggaaatgtga agaatttcag
      841 aaattttatt ccgtcatgac acaggcaccc tccg
//
LOCUS       DAZFRN010000424          866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFRN010000424 DAZFRN010000000
VERSION     DAZFRN010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.428"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..96)
                     /gene="ykgR"
                     /locus_tag="ACHKUB_005336"
     CDS             complement(<1..96)
                     /gene="ykgR"
                     /locus_tag="ACHKUB_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO6658180.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHF"
     gene            complement(570..659)
                     /locus_tag="ACHKUB_005337"
                     /pseudo
     CDS             complement(570..659)
                     /locus_tag="ACHKUB_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          253 a          151 c          189 g          273 t
ORIGIN      
        1 gaaatggaga aataaatagg cgtattctac aattgcgaca aaaacaacga tattaatcag
       61 tttatgactg atttgttgta ctttattctc tttcattggt acttcctcgc tttaaaaaag
      121 agcgcacttc gtaagtgccc ttatataaat aacgagtttg gtcaaccaat tttttgacat
      181 gtatcacaaa tttgaataga tgtattacat cggctatctt ttattgcacc aacgtcattg
      241 atatatgtcg cctgaagtca gttccgggaa tgagtctgat ctcaagactg gcccagtccg
      301 ggcgttgatt ggcgctgagg agcatatcgc atctcatcat aatgtcgtat ctcctggggt
      361 gttatacaag atatcgttgt tggtgacctg ggagaggaat tgagttctat taaaccgtca
      421 actatgccgg atacatactg gattacactg caggcacgcc ttacgagaga acgtgccgca
      481 gtgacgggtt aattatctga aagaatttgt gaggctgtat cggttactca ttgatttgat
      541 agttttactc tcgggagagt aatagatatt taatctatta acggaaacca gccagttcct
      601 ttcgatgcct gaatttgatt ccatagttta tccggaatgg ttaattcatg aataagttgt
      661 gttctcgttc atgagaagca taacgtaaag ggaaaagctc gattagacgg caggatttgt
      721 caggggttat gaacgaaatt cataaatctg tttgagtgtt gcgatgggta gtgcaagttc
      781 gaaatctccg caatttacag tccgatgaag gaaaatgaat atccataaaa aatatatcga
      841 tttattctgg cgtatatacc tacttc
//
LOCUS       DAZFRN010000425          848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFRN010000425 DAZFRN010000000
VERSION     DAZFRN010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.429"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>848
                     /locus_tag="ACHKUB_005338"
     CDS             <1..>848
                     /locus_tag="ACHKUB_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFO6658181.1"
                     /translation="IVEKGQVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSE
                     SLTFPDDVPADLRERESTVYRLRKTELAQSIAGLKQSKALLDKEIAMTRPIVREGAMS
                     EVELLRMQRQSAELQLQMDEKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSR
                     IRAPLRGIVKNIRVNTLGGVVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPAL
                     VKLTAYDYAIYGGLDGVVTLVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDR
                     NGKILPV"
BASE COUNT          253 a          165 c          244 g          186 t
ORIGIN      
        1 attgttgaga aaggtcaggt actgctgaca ctggatgata cccgaagctc tgccatgctg
       61 agggaaagtg aagcccgggt gaataacctg gaagcagtca gggcaagact ccgtgcagaa
      121 gcttattccg aatcactgac atttccggat gacgtgcctg cagatttgag ggagagggaa
      181 agtactgttt atcgactacg aaagactgaa ctggcacaaa gcatcgccgg ccttaagcag
      241 agtaaggctc tgctggataa ggaaatagca atgacccggc caattgtgcg tgaaggggcg
      301 atgtcggaag tcgaactgtt acgtatgcag cgtcagtcag cggagctgca gttacagatg
      361 gatgagaaac agaataaata cctgacggaa gctggagctg aactagtgaa gacagaagca
      421 gagcttgcac aggcaaaaga gaatatggct ggcagagccg acccggttga gcgttcacgt
      481 atcagggctc ccctgagagg catcgtaaaa aatattcggg taaatacttt gggaggcgtg
      541 gtttctgcag gacaggacat aatggaaatt attccgctgg aagaccagtt gctgattgag
      601 gcttatataa atccacggga tgttgcctat gtcagaacag ggatgccggc acttgtaaaa
      661 ctgacggctt atgactatgc catttatggt ggtcttgacg gagtggtgac actggtaagt
      721 ccggatactt tacgtgacca gaaacggccg ggagatttaa aactggatcc aaatgaagca
      781 tattatcgag tactggttac taccagtaat aattatctga cagacaggaa cggaaaaata
      841 ttgccagt
//
LOCUS       DAZFRN010000426          848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFRN010000426 DAZFRN010000000
VERSION     DAZFRN010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.430"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(278..>848)
                     /locus_tag="ACHKUB_005339"
     CDS             complement(278..>848)
                     /locus_tag="ACHKUB_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6658182.1"
                     /translation="LAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNPELIYCWE
                     QLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEPGTK
                     WTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIAREN
                     GYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          247 a          173 c          157 g          271 t
ORIGIN      
        1 ccggatatct aatgtcataa gcatcccttt aattatcccc tcaacctctt gtaactttcc
       61 cactcttgaa ctccacatag tccaaacgcc ggatgcaaaa aaatcacacg tgcatttctg
      121 aaattgtgat ggaggacgat agtatcaatt atatgtgctt ataaacgtcc ccgattagcg
      181 cagaagtcac cggattgttc aggctccaat aggttgagta tgccgtattt attttccaaa
      241 ataaaaatcg atgctgttgc tccttcataa aatactttta ctctctggac gcttttctta
      301 gctctcttgg tgttatctta aaacgattat ggaacctttc agtaaaacga gaaggacact
      361 tgtaaccatt ttccctggca atctcgctga tgggttttac tgtcgtttgt atagcagaca
      421 acgcattatt taacctcacg tcgtccaata tactttgaaa acttactccc tcgcttgcca
      481 gacggcgatg caatgtggaa acagaaatgt agagatatct cgctaccttg tttgctgtcc
      541 attttgttcc aggttcggat agcattaggt tataacaacg acttattaat gattgtttac
      601 tatctgataa aagtaaacaa ttaacatgat gtgctcctaa tgaaagtaga acgcccattg
      661 ctaagtgttc ctgaatttgc gttgagaagc ctcgggaaat agatgttttt agttgctccc
      721 aacaatatat taactcggga ttctgaggta agcagaaacc tgtcgtgtta cgaatttgat
      781 cggttatcgc ataaagattt tggaactttt caattagatc aatgggataa taaagcattt
      841 ctgcaaga
//
LOCUS       DAZFRN010000427          835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFRN010000427 DAZFRN010000000
VERSION     DAZFRN010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.431"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            197..799
                     /gene="fimB"
                     /locus_tag="ACHKUB_005340"
     CDS             197..799
                     /gene="fimB"
                     /locus_tag="ACHKUB_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO6658183.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          242 a          158 c          188 g          247 t
ORIGIN      
        1 aagagcatgg cgtttgtatg gcaacgttat tataattaac tgttgctact ccatttaagt
       61 tcactcagaa gaactggtcc acttacgtta gttattaagc aaacgttcgc ttttataaac
      121 ataatcagga taaaaatgtt ggattattgc taacccagca cagctagtgc gcgtctgtaa
      181 ttataaggga aaaacgatga agaataaggc tgataacaaa aaaaggaact tcctgaccca
      241 tagtgaaatc gaatcactcc ttaaagcagc aaataccggg cctcatgcag cacgtaatta
      301 ttgtctgact ttgctttgtt ttattcatgg tttccgggcg agtgaaattt gtcgattgag
      361 gatttcggat attgatctta aggcaaagtg tatatatatc catcgattaa aaaaaggctt
      421 ttcaacaacg catccgctat tgaacaaaga agttcaggct ttaaaaaact ggttgagtat
      481 ccgtacttcg tacccgcatg ctgagagcga gtgggtattt ttatctcgta agggaaatcc
      541 gctttctcgg caacagtttt accatattat ctcgacttcc ggtggtaatg ccgggttgtc
      601 actggagatt catccgcaca tgttacgcca ttcgtgtggt tttgctttgg cgaatatggg
      661 aatagatacg cgacttatcc aggattatct tgggcatcgc aatattcgtc atactgtctg
      721 gtataccgcc agcaatgcag ggcgttttta cggcatctgg gatagagcca gaggacgaca
      781 gcgtcacgct gttttataga gatagttgag ataccaggga tggtgttaaa aacta
//
LOCUS       DAZFRN010000428          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFRN010000428 DAZFRN010000000
VERSION     DAZFRN010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.432"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>834
                     /gene="yfdF"
                     /locus_tag="ACHKUB_005341"
     CDS             <1..>834
                     /gene="yfdF"
                     /locus_tag="ACHKUB_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO6658184.1"
                     /translation="PESINENNNDEVNGLVQELKNLFNGKEGISTCIKHLLELIKNAI
                     RVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPANYKDKELVRTIINDN
                     IVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTW
                     YGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARSAS
                     AWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRR
                     D"
BASE COUNT          314 a          145 c          158 g          217 t
ORIGIN      
        1 cccagaatcc attaatgaaa ataacaatga tgaagttaat ggattagtac aagagctcaa
       61 aaaccttttt aatggtaagg aaggaataag cacctgtatt aaacatctac ttgagcttat
      121 aaaaaacgcc atacgagtaa acgacgatcc ttatagattt aatattaata attcctcagt
      181 tacttatatt gatattggct ccaatgatac agaccatatt actattggta tcgacaacca
      241 agaaccaata gaattacctg cgaactataa agacaaagaa ctcgtccgta ctatcattaa
      301 tgacaacata gttgagaaga ctcatgatat caataacaag gaaatgatct tcagcgcatt
      361 aaaagaaata tatgatggag atcctggttt tatcttcgat aagatatcac acaaactcag
      421 acatacggta acggaatttg atgagagcgg gaatagcgaa ccaacggact tatttacctg
      481 gtacggtaaa gataaaaaag gcgactctct cgctattgta attaaaaata aaaacggaaa
      541 tgattactta tctctcggtt actacgatca ggacgactac cacattcaaa gaggaattcg
      601 tattaatggt gatagtctca cccaatattg tagtgaaaac gccaggagtg cttcagcgtg
      661 gtttgaaagc agcaaagcta tcatggcaga atcatttgca acaggttccg atcatcaggt
      721 tgtaaacgag ctcaacgggg aaagactgag agaacctaac gaagttttta aacgtttagg
      781 tcgagcaata agatataatt ttcaagtgga cgatgcaaaa tttagacgcg ataa
//
LOCUS       DAZFRN010000429          833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFRN010000429 DAZFRN010000000
VERSION     DAZFRN010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.433"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            46..>833
                     /locus_tag="ACHKUB_005342"
     CDS             46..>833
                     /locus_tag="ACHKUB_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6658185.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLS"
BASE COUNT          274 a          165 c          161 g          233 t
ORIGIN      
        1 aaactatgtg atctccattt cgattgattt agtgtttatt gacgtatgta ctggattatt
       61 aacgataata tcgagttctg gcctgagcac cgaaaattaa tatcggtaca taacgccgat
      121 cttaacgtcg ttctgacaac gccagccagt cgatgtttat cacttctact tgaagctttt
      181 cctgatgtgg ttgcacaaca agattttttc accagagtct gggaagaaga aggtatgcgt
      241 gtgcctacta acacgttata tcagaacata tccattatca gacgcggatt tcgcgctgtt
      301 ggtgatacta cccactcgct aattgcaacc gtgccgagaa gaggattcaa gatccataat
      361 gacattaaca tacaaaatca tgtaataaac tcgtcaacag acgcacatac acacaatgcc
      421 ccacctgcca taaaagttaa tgcgggatac aaagagagca ttggtggcgc aaagaatttc
      481 aataacaaaa tcctcaaaca tataaaaagt catctaatta tgttgagcgc atttgtcata
      541 ggcgcatatt ctgcatattg gctatggaat aataatcaac cgaagccatt tttcaaggat
      601 tacaagactg ttgcggaaat taatggctgc cattttaatg taacagaaga tacaattgac
      661 gggttgaaag agttcgataa atataaaaca cggatactgg attcggggat taactgtaaa
      721 aaacatccgt ggttatactt ccctcttgca aaatcctcac cggggatgat tgttatggca
      781 tgcaataaaa attataacca acatgaagtg gcagactgtt taaccctgtc tta
//
LOCUS       DAZFRN010000430          830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFRN010000430 DAZFRN010000000
VERSION     DAZFRN010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.434"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..152
                     /locus_tag="ACHKUB_005343"
     CDS             <1..152
                     /locus_tag="ACHKUB_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO6658186.1"
                     /translation="EEEKPYLITELPDSWVVEGAKLPYEVAGGVFIIEINKKNGCVLN
                     FLHSK"
     gene            155..434
                     /locus_tag="ACHKUB_005344"
                     /pseudo
     CDS             155..434
                     /locus_tag="ACHKUB_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            408..>830
                     /locus_tag="ACHKUB_005345"
     CDS             408..>830
                     /locus_tag="ACHKUB_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6658187.1"
                     /translation="MNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTYEDMKRLNL
                     GGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRKVKLSHSEM
                     IEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKTI"
BASE COUNT          266 a          129 c          225 g          210 t
ORIGIN      
        1 cagaagaaga aaaaccatat ttaattacgg aactaccaga tagttgggtt gttgagggag
       61 caaagttacc ttatgaagtt gcgggtggtg tatttattat agaaattaat aagaaaaatg
      121 gatgtgtttt gaatttccta catagtaaat aatgctggcg ctgatggatg cggatggaaa
      181 cattgcgtgg agcggggagt atgatgagtg gggcaaccag ctgaatgaag agaacccgca
      241 tcacctgcac cagccgtacc ggctgccggg gcagcagtat gataaggagt cggggctgta
      301 ctacaaccgg aaccggtact acgatccgtt gcaggggcgg tatatcactc aggacccgat
      361 agggctggag ggggggatgg agtctgtatg cgtacccgct gaatccggtg aatggtattg
      421 atccattagg gttaagtccc gcagatgtag cgctaataag aagaaaagat caactaaacc
      481 atcaaagagc atgggatata ttatctgata cttatgaaga tatgaagaga ttaaatttag
      541 gtgggactga tcaatttttc cattgtatgg cattttgtcg agtgtctaaa ttaaatgacg
      601 ctggtgttag ccgatcggcg aaagggctgg gttatgaaaa agagattaga gattacgggt
      661 taaatctgtt cggtatgtac ggcagaaaag taaagctatc ccattctgaa atgattgaag
      721 ataataaaaa agacttggct gtaaatgacc atgggttgac atgtccatca acaacagatt
      781 gctcagatag atgtagtgat tatattaatc cagagcataa aaaaacgata
//
LOCUS       DAZFRN010000431          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFRN010000431 DAZFRN010000000
VERSION     DAZFRN010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.435"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..258)
                     /gene="ybfB"
                     /locus_tag="ACHKUB_005346"
     CDS             complement(<1..258)
                     /gene="ybfB"
                     /locus_tag="ACHKUB_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO6658188.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIII"
     gene            complement(258..>803)
                     /locus_tag="ACHKUB_005347"
     CDS             complement(258..>803)
                     /locus_tag="ACHKUB_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO6658189.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMG
                     GTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEA
                     AGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETC
                     KMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          253 a          184 c          121 g          245 t
ORIGIN      
        1 tattattatc gccataatat acttactatc aatccttacg ccaataatgt ctgatacagt
       61 ccctataaat attagagcaa taaagaatac tatgcctgtt ggaaaacata ttaccatcat
      121 tccataagac atgagactaa ttaactcgct cacatcacga gtggagtcta atagtgaaag
      181 tctatgcatg gaaaagaata gtattaaaag cgataaagcc agccatatag ctctgaataa
      241 aaaaataata tatttcatta catcactccg gtgaatacaa atctaataaa acgtccaatg
      301 ttatttccgg agcacttcat cttacatgtt tctaaagaat cattagacca gtcttggcag
      361 tttttaaata gccctaaatt tataggtgtg aatatatcag gcaatacaga ataacgcggc
      421 cttacctctt taaacttccc ttttaaacag ttctccacac aactattatc ggtttcaacg
      481 aggtcacaac ggccagcagc ttcttttgta tcattacttg ctttacctgg cccccataga
      541 ccatctcctt ttgattctcc ccgttggtct tggccaccac aagtatagtc ccctttacca
      601 tctgggacac aaagatattg atgatagaac ggattccccc atatatctgg accgcttctt
      661 tcaccagttc cgcccattga atgcagtggc ttaatgcatt ttagtgtttc taatcctaat
      721 ggatctatat ttgagatcgg attcaacgga tactgataaa aattccatcc ccccttcagc
      781 ccaatcggat cctgagtgat ata
//
LOCUS       DAZFRN010000432          802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFRN010000432 DAZFRN010000000
VERSION     DAZFRN010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.436"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(26..709)
                     /locus_tag="ACHKUB_005348"
     CDS             complement(26..709)
                     /locus_tag="ACHKUB_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6658190.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT          210 a          147 c          156 g          289 t
ORIGIN      
        1 cttgttataa gcgtactaac tgcatttatt ttttgcttct tttctgccga ttttcagcag
       61 agggaagatt gtcgaactga atattttcca tgttgatggc taacttcaga tatcccaggt
      121 tattgggcga tacacccttc agggtcaaat taatgtcttg ccattgacct ggtaataatt
      181 tcaatgtgga tttcattgat gtgttgaaaa gtaattgttg ccaccgggca aactctttgg
      241 catcgacagg tttttcacca ccccatgtcg cattaaaacc aatgtttgag ataggtgtac
      301 ttaataagtt gaccagtgag atatgaataa cactaccatt ctctgtggcg tcaatggata
      361 acactcgacc cgctaaagcc accgaaccgt tgttcaggat gaaatacttc gtttttgctg
      421 ggttaaactc ggtaaaaata aaagtatttt gttggtcttt aacggattta attacgtgag
      481 ttaaagtctt tgtactgttt attatgccag tttgttgttt ttgaatagtt tcgagttgac
      541 tctttagctg tgcttgttgt ttttcaagta gcacaatttt attattatgt tcttcttgtt
      601 gtttcagtaa actactttct ggcttgcctt cttcaatgta gtcacatccc gttaatgcgc
      661 agagaactga aacagtgaca gcactgaaaa aaatcttctt caagttcatg taaaaatcct
      721 tattgcctcc gaccagcgat caatgccggt agagttgtcc attgtattat tggtgatcta
      781 tgttgcttta cattaatgac ag
//
LOCUS       DAZFRN010000433          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFRN010000433 DAZFRN010000000
VERSION     DAZFRN010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.437"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            85..717
                     /gene="yqeH"
                     /locus_tag="ACHKUB_005349"
     CDS             85..717
                     /gene="yqeH"
                     /locus_tag="ACHKUB_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6658191.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          252 a          133 c          158 g          248 t
ORIGIN      
        1 gcaaaattac aaaatcatgg tgtattgaca tcatcaatca ccaatgaaat tatgcaatta
       61 tatacggata gggaggttct taacatgggg ctttgtagtc gttataaaag tcttacatgc
      121 aatagttgct caatgcattg ccaaataatg ccagaagagt caccgcgttt gcagtactgc
      181 gcaaactcgt gtttttgtat gtggcccgaa gaaagttcat attttaatcg tggtgtggta
      241 gaaggtatct taacaaaaaa ccacaatgcg agattaagcg gatatatttt tgtcgatttt
      301 tcagtaagtt ttttacgtct attccttgaa aaagactgga ttgactatct tgcaagtact
      361 gacatgggga ttgttttagt cagcgaccgc aatatgcaat cgttagctaa ttactggcga
      421 aaacacaact cagcaatatc tgctgtcata tataatgatg atggtcttga tgttgccaat
      481 gaaaagatca gacaactatt tattggtcgt tatttatcat ttaccagagg taacacatta
      541 actcagatgg aattcaccat aatggggtat atggtttctg gttataatcc atatcagatt
      601 gctgaagttc tggatatgga tatccgtagc atctacgcgt acaagcaacg aatcgaaaag
      661 agaatgggtg gtaaaataaa cgaattattt attcgttcac attcggtcca acattgatac
      721 tcaacgacca gccagaatca tactctgttt atacgggcat ttattgaaat gtccgtattt
      781 tataaatagg c
//
LOCUS       DAZFRN010000434          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFRN010000434 DAZFRN010000000
VERSION     DAZFRN010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.438"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            66..>781
                     /locus_tag="ACHKUB_005350"
     CDS             66..>781
                     /locus_tag="ACHKUB_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6658192.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIA"
BASE COUNT          254 a          168 c          142 g          217 t
ORIGIN      
        1 tatatacgca aaaaacattg attaagtgaa tataccatgg aagaaaaata taaccggagt
       61 agtgtatggc caatctctac gacttgaaaa agttcgactt aaaccttctt gtcatatttg
      121 agtgtattta ccagcattta agcattagca aagctgctga atcactatat atcaccccat
      181 cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct ttattcattc
      241 gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga
      301 aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa
      361 actttattat ttacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct
      421 gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga
      481 atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga
      541 tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta
      601 ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca
      661 cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt
      721 ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaatcatt aatagcattg
      781 c
//
LOCUS       DAZFRN010000435          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFRN010000435 DAZFRN010000000
VERSION     DAZFRN010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.439"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            148..>780
                     /locus_tag="ACHKUB_005351"
     CDS             148..>780
                     /locus_tag="ACHKUB_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO6658193.1"
                     /translation="MKIALMNEFSQASKNAIIFNELVAASAHQGHTIFNVGMCDDNDH
                     RLTYVHLGIISAILLNSRSVDFVITGCGTGQGALMALNTFPGVTCGYCIEPTDAYLFA
                     QVNNGNALSLPFAKGFGWGAELNMRYIFEKAFDGEKGLGYPAERRESQNANAIILSNM
                     KEAVSKPLMDALQAIDPELLKQALGGEKFQKCFFNNSKDKELVNYVKNLLD"
BASE COUNT          249 a          124 c          170 g          237 t
ORIGIN      
        1 gtgatcgaga gcacacaaca ataaaaatga aatgatgttt tgaattttca aatacaatgt
       61 tgcaagtaat gcttgtaatg tattatcata tcgttcatgt tgttatgcta tgaagtttaa
      121 cagtatgatt ttaaatgagg aattcgtatg aaaatcgcat tgatgaatga atttagccag
      181 gcatctaaaa acgcaataat ttttaatgag ttagttgcag caagcgcaca tcaaggccat
      241 actattttca atgtaggaat gtgtgatgac aatgatcatc gtctgactta cgttcatctc
      301 ggcattattt cggctatctt gttaaacagc agatctgttg atttcgttat tactggctgt
      361 ggtacaggac agggggcatt gatggcactg aacacattcc cgggagttac ttgtggttat
      421 tgcatcgaac caacagatgc ctacctgttt gcacaggtaa ataatgggaa tgcactgtcc
      481 ttgccatttg caaagggttt tggctggggg gcagaattaa acatgcgcta tatttttgaa
      541 aaagcatttg atggcgaaaa gggattaggc tatccggcag aacgtcgtga atcacaaaat
      601 gcgaatgcca ttatactgag taatatgaaa gaagcagtta gtaagccact catggatgct
      661 ctccaggcca tcgatccgga gttacttaaa caagctcttg gtggcgaaaa attccagaaa
      721 tgcttcttta ataatagtaa agataaagaa ttggttaatt acgttaaaaa tttattagat
//
LOCUS       DAZFRN010000436          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFRN010000436 DAZFRN010000000
VERSION     DAZFRN010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.440"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            18..518
                     /locus_tag="ACHKUB_005352"
     CDS             18..518
                     /locus_tag="ACHKUB_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6658194.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLNPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            520..>775
                     /locus_tag="ACHKUB_005353"
     CDS             520..>775
                     /locus_tag="ACHKUB_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO6658195.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTIENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMY"
BASE COUNT          274 a          139 c          132 g          230 t
ORIGIN      
        1 agagtacagg ttggcatatg caagtctttt ccagtgatgt ctacttcact gtaggcacta
       61 atgcattatt agcatcccaa aaagaatatt acagcgattt agttgcgttg gttgatcttg
      121 ggcattcgtt cgttgttatt gatgagcatc aacatcgaaa tctcaatcca aatacagaac
      181 ctgtaaacat ccttttaagc aacaatttca tcaggatcaa taagaatatt acattatctg
      241 atttaacgca tttcctgatt tcgaatcttc atacacaaaa tgtgtattcc acacaagaac
      301 cacttaccca tgatgagata gatatcctgc ggttatgtgt ttcttactcg ttaaaacaaa
      361 tagccattat taaaggaatt gattataaaa caatatcata tcacaaaata agagccctaa
      421 ataaacttaa cataaaaggt actgttgagt tgtttatagc actctgtgaa tgggataaac
      481 attattttaa actccaatct tgcgtaagag aaagttgata tgtcattagt aggatataat
      541 agtaacatca taataaccat cgcgctgata tcgttgctct ctgcatgcac catagaaaat
      601 tcattaaagg agaatcaaaa taaaagaagt ggcgaatccc aagcgatagt agatgcaaga
      661 cagttcttta gcgaacaccc ggaatatata tcttcagcag aactggagca aaagctatat
      721 cgcgaatttg cacaggttac tacagttcca gcttataaag agatgagtat gtatc
//
LOCUS       DAZFRN010000437          764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFRN010000437 DAZFRN010000000
VERSION     DAZFRN010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.441"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(1..711)
                     /locus_tag="ACHKUB_005354"
                     /pseudo
     CDS             complement(1..711)
                     /locus_tag="ACHKUB_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT          253 a          130 c          154 g          227 t
ORIGIN      
        1 gaaagtaaaa aagaaggaaa atacagcaat ggaaaaaatt ttatcggaga acaggaataa
       61 atctaaaaga tagattgctg aaatatagac tccactttta catgcgcgac aaaaattact
      121 gccttgatga aaaaaccaga aagggaagaa catcactgca aatacccaaa aaatagcaaa
      181 gtaataatga tcactggaag acgctgattc actttctgaa ggattagtcg gatattggtg
      241 ttgtgaagtg agttggttat tcaaagcgtt tatagctagc tctaatccct gagcaaattt
      301 ttgttgttta aatgcgggta tcatgttgct acggatgata tctctagcca gagcatcggt
      361 taccttttct tccagcccat aacctacctg gatgcggaca gtacgatctg accaggcaac
      421 aatgatcagt atcccatcat tacgtttggc atctcctaaa cgccaattgt caaaaactct
      481 tgtagcatat tgttcaattg tctcgtcttt ggttgtggga acaattaata cagcaatttg
      541 cacttgggta ttattgaaaa tagattctaa cttgtgcgtc agttctttct tttcttgtga
      601 gtttaagcta ctcgtaagat ccgtaacttg ttactgtaat tgtgggacaa tagttggttg
      661 ggctgcaaca aaggaacata cgaaagtgaa cattagtatg aaatagcgca ttataaattt
      721 ctcattaaat ctggataaat aaattgacgg cggcatcggt aatt
//
LOCUS       DAZFRN010000438          754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFRN010000438 DAZFRN010000000
VERSION     DAZFRN010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.442"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(190..732)
                     /gene="fimA"
                     /locus_tag="ACHKUB_005355"
     CDS             complement(190..732)
                     /gene="fimA"
                     /locus_tag="ACHKUB_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6658196.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          232 a          155 c          153 g          214 t
ORIGIN      
        1 aaaaataaga aatataaaga aatgattgct ttggtttctt acaacacatc attccctttt
       61 catttaataa agcggcatga tacaaagtga acatgatttc gatttacatt gccaccatac
      121 cgcttcagac aaccggtaca ttactttgtt ataagacgtg atatataatt caaaacaacg
      181 tggttttgat tattcgtatc tcataataaa agtcgcgtca gcatttgctt gccctgctga
      241 tgcacttgta ccggtgcctt tataacgtgc agaaaaatga agaacgttgg tcccggggat
      301 caagttctgg ttggttgaga agctattccc atcaggtttg agaattgcgg atgtattatc
      361 aagtatttca ataccgacac ccgttgctgt tgttgtattg gtactggatg caatggccaa
      421 taaattatca ttgattgcat cagcctgacc agaaaatgca acggcagcat tagcggcaac
      481 aacaggatcg cagtcgttca actgaatggt gaatggaatt aatgcagagg tattaccaac
      541 agcattgaaa atatcggtac gatattgacc gagtgtgaca acctggtctg ccgagtcagt
      601 attcactgaa caggcagcat tcaccagttc gccttcaaag tgaattgtac cgccatctac
      661 aacggcagca taactacccg tagcggcgaa tagtgcggca gccagcgcag acgaaataaa
      721 tcttaatttc atatatattc cttcaatctc attt
//
LOCUS       DAZFRN010000439          753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFRN010000439 DAZFRN010000000
VERSION     DAZFRN010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.443"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..233)
                     /locus_tag="ACHKUB_005356"
     CDS             complement(<1..233)
                     /locus_tag="ACHKUB_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO6658197.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSG"
     gene            414..566
                     /locus_tag="ACHKUB_005357"
                     /pseudo
     CDS             414..566
                     /locus_tag="ACHKUB_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          241 a          134 c          152 g          226 t
ORIGIN      
        1 ccggaacttg aggcactaaa agcaacatta atagaacctg ggccagggaa atggtattta
       61 ataagtctgt aaggatcaac ttttgctgag atatcgctta ggccgagtga gagaaagtct
      121 tgttctacca tatcaccata ttgcatatca tcagcagaaa aatcatcgaa taaactaata
      181 gtttcgaaaa gtgtacaggg aatattaatt ggcggaagta atatgttatt catttgcatt
      241 tgccttggtt atatttaagc tcaatgtcaa ttcaagaaca aatgctaggg taaaaaaata
      301 tttaaaaaag aagtacattt cacatttttt cattacatct ctgaagagaa cctcgttaat
      361 gattggtctt gctcattgtt aataacaaaa tgttgaattt accgattagg aaactttttc
      421 ctacgcggat tgcctccagc tatggcatga actttgagtt tatgccagaa ctgaagtgga
      481 gctttggcta ccctggcgcg attatcttta tgatccttgc gggcctgaca ccgtatctgt
      541 actttaagcg taagaactgg ctgtaactat acgatgtcgc tgtgagtaag ttagtgtgct
      601 gacagcgaca ttttagccga gggaatcgca gataatataa ttgttgtgaa taatatccat
      661 gccacgttga gttggacgct taatgactca catctcaccc cttcaattgc tgcagagata
      721 atagaaaaaa gtgtcaaacc caatgaaatc aat
//
LOCUS       DAZFRN010000440          749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFRN010000440 DAZFRN010000000
VERSION     DAZFRN010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.444"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..193
                     /locus_tag="ACHKUB_005358"
     CDS             <1..193
                     /locus_tag="ACHKUB_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO6658198.1"
                     /translation="PEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIA
                     PNERGDKKGVPGLSCFQPH"
     gene            455..>749
                     /locus_tag="ACHKUB_005359"
     CDS             455..>749
                     /locus_tag="ACHKUB_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658199.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAH"
BASE COUNT          257 a          120 c          151 g          221 t
ORIGIN      
        1 acctgaagat atcttcaaat gtggacagac gctcaaaaaa actaaacaga aatatggcca
       61 ttatggatta ggggtagatc agcatcaaaa aattgataat tataccatgg ggctaaattc
      121 ctggaagata gctcctaacg aacgtggtga taaaaaaggt gtcccagggc ttagttgttt
      181 tcaaccacat taaaactcca tgagtgctgt ggtatatctt tttaatcctg ccagtatttc
      241 ataggttttc ttaatcgatt gtagaggcat gttctgatga cgcctctaca atctttaggg
      301 actttctctg ctatgagata agaaatttca tattatgctt taaggctgaa tgatttgtgc
      361 aaaatataat tatttttcca tcgaacatga ggtgtatgtc ataagattaa atgttaacta
      421 tgcattcctc gcggtgaatt atatgaggta aaagatgaaa attggaactg tggcaggcac
      481 taacgacagt acaacgacaa tcgcaacaaa tgatatggtg caagagcatg ttactaactt
      541 cactaaagaa ctgtttgggt atattgcaaa tggtataggt gatgatatat caagtattgc
      601 caggacaatg ctcggtgaag ttgtggaaaa aattgatgat tggcaaatag aacgtttcca
      661 acaatctatt cgagatgaca aaatatcatt taccatacaa acgaatcatt cagaaaaata
      721 tagtatgcta tcggacatgc gcgctcata
//
LOCUS       DAZFRN010000441          748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFRN010000441 DAZFRN010000000
VERSION     DAZFRN010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.445"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            14..301
                     /locus_tag="ACHKUB_005360"
     CDS             14..301
                     /locus_tag="ACHKUB_005360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6658200.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSISADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            314..>748
                     /locus_tag="ACHKUB_005361"
     CDS             314..>748
                     /locus_tag="ACHKUB_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFO6658201.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTG"
BASE COUNT          186 a          151 c          205 g          206 t
ORIGIN      
        1 ctgaggaaca tttatgaaaa ttacagtggt ttgcggtaac ggcttaggca ccagcctgat
       61 gatggaaatg agcattaaaa gcattttgaa ggatttgtcc atcagcgctg atgttgatca
      121 tgtcgacctg ggctccgcga aagggacgcc gagtgatatc tatgtcggca ccaaagatat
      181 tgccgagcag ttagtcgcgc agtctgtcgc aggcaaaatc gtcgccctgg aaaatatgat
      241 tgataagaaa gccatgagag aacgtttgtc cgtggcatta accgaattag gcgcactgta
      301 agctgggggc gatatggatt tctttcgttt tttgatgagc gatgtacttt ccgaacccgc
      361 ggtgctggtc ggtttaatcg ccctgattgg cctgattgca cagaaaaaac cggtaaccga
      421 atgcattaaa ggtaccgtca agaccattat ggggtttgtg attttaggcg caggggcggg
      481 tttagttgtg tcgtcactgg gtgacttcgc aaacattttc cagcatgcct ttggtattca
      541 gggtgtcgtg ccgaacaatg aagccattgt ttccgtagcg cagaaaagct tcgggaaaga
      601 aatggcgatg attatgttct ttgcgatggt tatcaacatt atgattgccc gtttcacacc
      661 gtggaaattt atcttcctga ccggtcatca cacgttgttt atgtcgatga tggtagcggt
      721 aattctggca acagcaggca tgaccggc
//
LOCUS       DAZFRN010000442          748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFRN010000442 DAZFRN010000000
VERSION     DAZFRN010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.446"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            14..301
                     /locus_tag="ACHKUB_005362"
     CDS             14..301
                     /locus_tag="ACHKUB_005362"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6658202.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            314..>748
                     /locus_tag="ACHKUB_005363"
     CDS             314..>748
                     /locus_tag="ACHKUB_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFO6658203.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLSDFANIFQYAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTG"
BASE COUNT          190 a          149 c          203 g          206 t
ORIGIN      
        1 ctgaggaaca tttatgaaaa ttacagtggt ttgcggtaac ggcttaggca ccagcctgat
       61 gatggaaatg agcattaaaa gcattttgaa ggatttgtcc gtcagcgctg atgttgatca
      121 tgtcgacctg ggctccgcga aagggacgcc gagtgatatc tatgtcggca ccaaagacat
      181 tgccgagcag ttagtcgcgc agtctgtcgc aggcaaaatc gtcgcactgg aaaatatgat
      241 tgataagaaa gccatgagag aacgtttatc cgtggcatta accgaattag gcgcactgta
      301 agctgggggc gatatggatt tctttcgttt tttgatgagc gatgtacttt ccgaacccgc
      361 agtgctggtc ggtttaatcg ccctgattgg cctgattgca cagaaaaaac cggtaaccga
      421 atgcattaaa ggtaccgtca agaccattat ggggtttgtg attttaggcg caggggcggg
      481 tttagttgtg tcgtcactga gtgacttcgc aaacattttc cagtatgcct ttggtattca
      541 gggtgtagtg ccgaacaatg aagccattgt ttccgtagcg cagaaaagct tcgggaaaga
      601 aatggcgatg attatgttct ttgcgatggt tatcaacatt atgattgccc gtttcacacc
      661 gtggaaattt atcttcctga ccggtcatca cacgttgttt atgtcgatga tggtagcggt
      721 aattctggca acagcaggca tgaccggc
//
LOCUS       DAZFRN010000443          748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFRN010000443 DAZFRN010000000
VERSION     DAZFRN010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.447"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>748
                     /locus_tag="ACHKUB_005364"
     CDS             <1..>748
                     /locus_tag="ACHKUB_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6658204.1"
                     /translation="LEGDLLRTEVAGMSVTAGIYGAAGHSSVDVKDDDASRAGTVRDD
                     AGSLGGYLNLTHTSSGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSI
                     TDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGYGSAQHVRAGFRLGSHNDMTFGEGTS
                     SRDTLRDSTKHGVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSL
                     DLQAGLEARVRENITLGVQAGYAHSVSGSSA"
BASE COUNT          154 a          210 c          232 g          152 t
ORIGIN      
        1 ctggagggtg acctgctgag aacagaggtt gccggtatgt ctgtgaccgc agggatatat
       61 ggtgctgcag gccattcttc cgttgatgtt aaggatgatg acgcctcccg tgccggcacg
      121 gtccgggatg atgccggcag ccttggcgga tacctgaatc tgacacatac atcctccggc
      181 ctgtgggctg acattgtggc cctgggaacc cgccacagca tgaaagcgtc aacggacaat
      241 aacgacttcc gcgcccgggg gtggggctgg ctgggttcac tggaaaccgg tctgcccttc
      301 agtatcactg acaatctgat gctggagccg caactgcagt acacctggca gggactctcc
      361 ctggatgacg gtaaggataa cgccggttat gtgaagttcg ggtatggcag tgcacaacat
      421 gtgcgtgccg gtttccgtct gggcagccac aacgatatga cctttggtga aggcacctca
      481 tcccgtgaca ccctgcgcga cagtacaaaa cacggtgtga gtgaactgcc tgtgaactgg
      541 tgggtacaac cttctgttat ccgcaccttc agctcccggg gggacatgag catgggtaca
      601 gctgcagccg gcagtaacat gacgttctca ccgtcccgga atggcacgtc actggacctg
      661 caggccggac tggaagcccg tgtccgggaa aatatcaccc tgggcgttca ggccggttat
      721 gcccacagcg tcagcggcag cagcgctg
//
LOCUS       DAZFRN010000444          734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFRN010000444 DAZFRN010000000
VERSION     DAZFRN010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.448"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..66
                     /locus_tag="ACHKUB_005365"
     CDS             <1..66
                     /locus_tag="ACHKUB_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658205.1"
                     /translation="EQIEPNLLGVYAGLLLAMKNK"
     gene            complement(102..>734)
                     /locus_tag="ACHKUB_005366"
     CDS             complement(102..>734)
                     /locus_tag="ACHKUB_005366"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6658206.1"
                     /translation="DLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQS
                     FEKLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHC
                     YYPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMT
                     ADSISSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          221 a          157 c          126 g          230 t
ORIGIN      
        1 gaacaaattg aaccaaattt attaggcgtt tacgccggct tattattagc gatgaaaaac
       61 aagtaaaaaa taaaaaaggc tgcataatgc ggccttttta tttattcaaa ccaattatga
      121 ataacctctt cgaatctttg agttacgcca tcccaactgt agtgttcaaa cacaaagtcc
      181 tgaccttgct ttgcaactgc tgttaattca ggattcgcta acgtttttaa aatatcgctg
      241 cttatggagt ctgcagtcat aggttctttt aaatggaatc ccgtagtatt ttctttgaca
      301 aattccgtca tccccccacg cgtactcact aaaacaggct taccagcacc catagcttca
      361 attgccacca tgcaaaatgg ttcctggaac tgggaaggaa tgacgactaa atctgccagt
      421 gggtaataac aatgcatctt ttcaggcggt atgcttccta acataatgca acgatccttc
      481 aaacgttttg caatttccct gacattgcgt tgatatgcac ctttgtcact tttactcatt
      541 tctgtataat caccaatcac aactaatttt aaatttttgt gagcagcggc caatttttcg
      601 aatgattgca tcaacagcaa tatgcctttg tcagggacga tacgcccagc ataaaaaata
      661 attttctctt caggggagat attaagttcg cttctagtga tcggctgaaa attagattga
      721 taggtttcta aatc
//
LOCUS       DAZFRN010000445          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFRN010000445 DAZFRN010000000
VERSION     DAZFRN010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.449"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(175..714)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKUB_005367"
     CDS             complement(175..714)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKUB_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO6658207.1"
                     /translation="MASLMLAGHALAEDGVVHFVGEIVDTTCEVTSDTADQIVPLGKV
                     SKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGTPLTAGNP
                     GDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIARYVATNA
                     TVTAGTANADSQFTVEYKK"
BASE COUNT          201 a          176 c          154 g          185 t
ORIGIN      
        1 caccaatgac aacgcctgca taagttaatg ctggttggcc taaaaaaacg ctgagtataa
       61 tcgcggtaaa taacttacat gaaactgttg ttttaaagag cctgattaaa caactttttc
      121 tggacataac gactccctgg ccaaagccaa ctgaataaaa aggcccttga taaattattt
      181 cttatattcg acagtaaatt gtgaatcagc gttcgccgta ccagccgtta cggtcgcatt
      241 ggttgccaca tagcgtgcaa taaacttcat ttcagccttg ccttcagcat cgatagcggt
      301 ataagcagag tcgttataaa gtttaacctg cgaattatcg gactggttaa aaatacgaat
      361 accaacgccg gttgccgcaa tcgcttgtcc tgtaccggta aaatcaccag ggttgcctgc
      421 tgtaagcggc gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg taccatcaaa
      481 acgaacggct gcttgagtgt acgttgccgg gcaattttca agcttaatac tgaacttctg
      541 tggcgacgcc agactaccta cacctgaaaa tgcattttta ctgactttac ccagtgggac
      601 aatttgatcg gctgtatcgg aggtaacttc acaagtagtg tcgacaattt caccgacgaa
      661 gtgaacaaca ccatcttctg ccagtgcatg cccggccagc attagagatg ccagag
//
LOCUS       DAZFRN010000446          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFRN010000446 DAZFRN010000000
VERSION     DAZFRN010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.450"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..316)
                     /locus_tag="ACHKUB_005368"
     CDS             complement(<1..316)
                     /locus_tag="ACHKUB_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFO6658208.1"
                     /translation="MITCVFIFTAILLAVITFIPVSGNTSWVVRVWDFPRLQILLLCT
                     LNVFLSVVFLDSFAELFVVIISLICVAYQLVWIIPYTVIGKKQVKEIKDSDGFPGLSI
                     LVF"
     gene            complement(579..>716)
                     /locus_tag="ACHKUB_005369"
     CDS             complement(579..>716)
                     /locus_tag="ACHKUB_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO6658209.1"
                     /translation="GRPLTGIFCLVLQITLLGWIPATIWAVYALSQYKTEQKIREATG
                     K"
BASE COUNT          234 a          191 c          117 g          174 t
ORIGIN      
        1 tgaataccag tatacttaaa cccggaaatc catcagagtc ctttatttcc tttacctgct
       61 tcttgccaat tactgtataa ggaattatcc ataccaactg gtaagcgaca caaattaaac
      121 ttattatcac aacaaacaac tctgcaaatg agtcaagaaa aacaacagac agaaaaacat
      181 tcaaagtaca cagcaaaagt atctgtagtc ggggaaaatc ccataccctg acaacccatg
      241 atgtattacc ggaaacaggg ataaaagtta tgactgccag aaggatagca gtaaaaataa
      301 aaacacaagt tatcacaaat cactccttgt tctgaaccgg cacacaaaac tgtcatatac
      361 gtttcaaaag taaaaacaca ctgctgccac aagattcacg acgtaaccgg gcagcatatc
      421 ctgattacgg acaatccatg aaaccgcctc gccagaagcg tccatcacat ccggtttttc
      481 cctgttttat attccacgaa ccattttatt ttcaggaatc tccgggcctt tatcccgcat
      541 catatacaca gctatgacaa cccgctcttc tgcagtcatc atttccccgt ggcttcgcgg
      601 attttctgtt ccgtcttata ctgcgaaagc gcatacacgg cccatattgt tgccggtatc
      661 cagcccagca gcgttatctg taacaccaga caaaaaatcc ccgtcagagg ccggcc
//
LOCUS       DAZFRN010000447          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFRN010000447 DAZFRN010000000
VERSION     DAZFRN010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.451"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            1..240
                     /locus_tag="ACHKUB_005370"
                     /pseudo
     CDS             1..240
                     /locus_tag="ACHKUB_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            279..>709
                     /locus_tag="ACHKUB_005371"
     CDS             279..>709
                     /locus_tag="ACHKUB_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO6658210.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALES"
BASE COUNT          252 a          149 c          119 g          189 t
ORIGIN      
        1 ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg taataaactc
       61 gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg tgacgatggt
      121 gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt
      181 ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa cggctcgcgc
      241 ggcaagaatc tctgattgag ttaagtgccc atcacaatat gatcactgac cttatattac
      301 acaatcatcc caggatgaaa acaatcactt taaacgacaa ccatattgca catttaaacg
      361 ccaaaaacac tacaaaactg gaatatttaa acttaagcaa taacaattta ctgccaacca
      421 atgacattga tcaactaata tcatcaaaac atctttggca tgtattagtt aacggcatca
      481 acaatgatcc acttgcacaa atgcagtact ggactgcagt aagaaatata attgatgaca
      541 ctaatgaagt gaccattgat ttatcaggac ttaatttaac cactcaacca ccagggctgc
      601 aaaacttcac atctatcaat cttgataata accaactcac acattttgat gcaaccaact
      661 acgatagact cgtaaagcta agtctgaata gtaatgctct tgagtcaat
//
LOCUS       DAZFRN010000448          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFRN010000448 DAZFRN010000000
VERSION     DAZFRN010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.452"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>698
                     /locus_tag="ACHKUB_005372"
     CDS             <1..>698
                     /locus_tag="ACHKUB_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO6658211.1"
                     /translation="LVVGVAMVFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIG
                     SKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNW
                     FMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAP
                     NAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAIL
                     GAFAQGLLITFLPV"
BASE COUNT          131 a          160 c          190 g          217 t
ORIGIN      
        1 ctttggttgt tggcgtggca atggtctttt tcccggctat tgcgcatccg tacatgaaga
       61 aagtgaccgg ttctgatgac gttgccatcg gccacttctc aacattatct tatgtattgg
      121 ccggtttcat cggcagcaag tttggtaata aagagcactc gactgaagac atgaatgtgc
      181 cgaaaagtct gctgttcttg cgcgacacgc cggttgctat ctcttttacc atgagcataa
      241 ttttcctggt gacgtgcctg tttgcgggtg cagatgcggt gaaagagctt agcggtggta
      301 aaaactggtt catgttctct atcatgcaat ccattacctt cgcagctggc gtgtacatca
      361 ttctgcaggg tgtgcgcatg gtgattgcgg agattgtccc ggcattcaaa ggtatttcag
      421 acaagctggt tccgaatgcc agacctgctc tcgattgccc ggtcgtcttc ccttacgcac
      481 ctaacgctgt actggttggt ttcctgagta gctttgctgc aggtttaatc ggtatgttca
      541 cgttgtatct gctgaacatg atcgtgatta ttcctggtgt ggtgccgcac ttcttcgtag
      601 gtgcagctgc aggggtattc ggtaatgcaa cgggtggacg tcgtggtgcg attttgggtg
      661 ccttcgctca gggcttgctt attaccttcc tgccagta
//
LOCUS       DAZFRN010000449          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFRN010000449 DAZFRN010000000
VERSION     DAZFRN010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.453"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>691
                     /locus_tag="ACHKUB_005373"
     CDS             <1..>691
                     /locus_tag="ACHKUB_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO6658212.1"
                     /translation="EENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDP
                     IGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDS
                     TAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEK
                     VSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEIT
                     NRKGLPNVCRVH"
BASE COUNT          229 a          129 c          161 g          172 t
ORIGIN      
        1 tgaagagaac ccgcatcagc tgcagcagct tatccggctg ccggggcagc agtatgatga
       61 ggagtccggc ctgtattaca accgccaccg ctattatgac ccgctgcagg ggcgatatat
      121 cactcaggat ccgattggac tgaagggggg atggaacctg tatggatatc aattgaatcc
      181 gatatcagac atcgaccccc tgggtttatc tatgtgggag gatgcaaaat cgggggcatg
      241 tactaatggt ctttgcggca cactatccgc tatgataggt ccagataaat ttgattctat
      301 agatagcacc gcatatgacg ccttaaataa aataaatagc caatctattt gcgaagataa
      361 agagttcgct ggtttaatat gtaaggataa tagtggcaga tatttctcaa cagcacctaa
      421 ccgaggagaa agaaaaggat catatccatt caatagccct tgccctaatg gtactgagaa
      481 agtatcagct tatcatactc atggtgcaga tagtcatgga gaatattggg acgaaatatt
      541 ttcaggtaaa gatgagaaaa tagttaaaag taaagataac aatatcaagt cattttattt
      601 aggtacgccc agtggtaatt ttaaagcaat agataaccac gggaaggaaa taacaaacag
      661 aaaaggatta cctaatgtct gcagagttca t
//
LOCUS       DAZFRN010000450          690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFRN010000450 DAZFRN010000000
VERSION     DAZFRN010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.454"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(248..>690)
                     /locus_tag="ACHKUB_005374"
     CDS             complement(248..>690)
                     /locus_tag="ACHKUB_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6658213.1"
                     /translation="QRVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRL
                     FRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDGLRTESVRSQAREQF
                     TGAVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          166 a          186 c          193 g          145 t
ORIGIN      
        1 gaggtccggc tgacggaagt ttctgaattg tggttctgtt gttccctgaa tctcatgtct
       61 gtgtgtatct gcgcacgttg ctaacaggag ttattattag cataacgatc gatattaacg
      121 atcggtgaaa taggtatcga tcgttttatt ctattgtcaa gctattgcac ggacaagaga
      181 gacagtaacg aaaacagcgc ttttgtgagc agacccagag gcagggagga catcatccac
      241 gacaggttca gaagaaaatc cagttccata ccgcacgggc aacagagacc accgtgtcac
      301 gaacagcgcg cagggccgta cggacaacag aggcaacaca gacgctctcc gctgtgtcaa
      361 atatccggtc caccgcaccg gtaaactgtt cacgggcctg agagcggaca gattccgtgc
      421 gcagcccgtc ctgcagtcgg gtcatcaggg gactggcggc atggtcggga agcgctgtca
      481 tgagcgcact gaccagcgta tccagttccc agccggtgcg ggccgatacg gccacaaccg
      541 gatgtacggg ccggaacaga cggaataccg cctccgtttt ctcccgaatg ttctgtgcct
      601 gtgcgggaga aggctgaata ccggccatat cccattcatg gcagggctcc gttttatcgg
      661 cctgcgtcac cacaaacagt acccgctgat
//
LOCUS       DAZFRN010000451          680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFRN010000451 DAZFRN010000000
VERSION     DAZFRN010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.455"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>680
                     /locus_tag="ACHKUB_005375"
     CDS             <1..>680
                     /locus_tag="ACHKUB_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6658214.1"
                     /translation="WANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELNTSLK
                     FVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSNVNRT
                     IANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRDVTEE
                     EGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVDAL
                     EDDDDVQK"
BASE COUNT          224 a          120 c          152 g          184 t
ORIGIN      
        1 atgggccaat attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc
       61 aaaattcggt gtagaaatct atgctgctgc taaacaaggt gaacccgatc cagaattaaa
      121 cacatcttta aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat
      181 tgataaagca attgataaag ccaaaggcgg cggagatgaa acgttcgtgc agggacgtta
      241 tgaaggcttt ggtcctaatg gctcaatgat tatcgccgag acattgactt caaatgttaa
      301 ccgtacgatt gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc
      361 aggttctgtc agctatatgt ttgacaatac gggtgtgatt gtatttaaag gatcagaccc
      421 tgaccatatt tttgaaattt tacttgaagc tgaagttgat gttcgtgatg tgactgaaga
      481 agaaggtaac attgttattt ataccgaacc tactgacctt cataaaggaa tcgcggctct
      541 aaaagcagct ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga
      601 agttgagctt tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga
      661 tgacgacgat gtacaaaaag
//
LOCUS       DAZFRN010000452          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFRN010000452 DAZFRN010000000
VERSION     DAZFRN010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.456"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..572)
                     /gene="fimE"
                     /locus_tag="ACHKUB_005376"
     CDS             complement(<1..572)
                     /gene="fimE"
                     /locus_tag="ACHKUB_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO6658215.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLIN"
BASE COUNT          199 a          171 c          139 g          162 t
ORIGIN      
        1 tcgtttatga gattatttct ttcccatagt ccggcaaaac gagcagcatt actggcggta
       61 taacgtacag tatggcgaat atttcgatgc ccgagataat cctgaattaa acgggtatcg
      121 gcaccacgct ccgccagttc ataaccacaa gcatgcctta acatatgagg atgagtctgc
      181 gttacggttc cagcttcaat accggcatcg cgaataatgc gataggcctg ctggcgagaa
      241 agccgactcc cgcggcgaga aataaatata gcgtcggtcc ggtcagcgcc tttccagtta
      301 gcacgttcct gggtccagcg ttccacggct tcacgctcat caaaacgtaa cgggtgaacg
      361 gtagaaaatc cgttcttcag tcggcgaata tttattcgac cttcattaag gtcaagatcc
      421 tgataatgca gatcaagcag ttcactaata cgcatcccat gccgatatgc caacagaata
      481 agacaataat ctctggctcc cgttgccccg taacaaaccg cctgcatcat ggcctgaact
      541 tctttaccgg taagataacg acgtttactc acaatagtag tactcctaac tgagatatat
      601 ttgaatgaat acctatagga aacctcaatc ggtcaaaatt agcccaatag ataaaaagat
      661 aaccacacac g
//
LOCUS       DAZFRN010000453          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFRN010000453 DAZFRN010000000
VERSION     DAZFRN010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.457"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>657
                     /locus_tag="ACHKUB_005377"
     CDS             <1..>657
                     /locus_tag="ACHKUB_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFO6658216.1"
                     /translation="LYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLT
                     DIAGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVD
                     LPFILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLL
                     VEALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVL
                     L"
BASE COUNT          142 a          149 c          186 g          180 t
ORIGIN      
        1 ctgtatgtca tgaacgttta tgaccgggtc atacccaatc gctcctggga aacgttatgg
       61 atgctgagta tcggggtgat tgcagccatt ctgtttgagt gtgctgcaaa gttaatccgg
      121 agctatctga cggatattgc gggtaagaag gcagacctga ttatcagctc agcactgtta
      181 aggcgggtga tggcagtgaa aatgtcggac aggccggcgt cttccggttc atacgccagc
      241 aatttgcggg aatatgagtc tgtcagggag tttatgacca gcgccacgct gctggctttt
      301 gtggatttgc cgtttatcct gcttttcatc ggtgtcatcg ggcttgtggg gggctggctt
      361 gctgttgttc ctcttacact ggttccgctg gtgattctgg caggaatatt actgcagcga
      421 ccgctttcgc gtcatattaa tgcctccatg cgggaatcat cgcagcgcca gggattactt
      481 gtggaggcac tggacggaat agaaaccctg aagctgaata atgcagttaa ctgggtccag
      541 cgccgctggg atggatatac agccagtgtc gcccgctcgt ccatacgggt aaaaaatacc
      601 agtaatgtca taatctgctt ttccacagca gttcagcaac tgaatactgt tctgctg
//
LOCUS       DAZFRN010000454          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFRN010000454 DAZFRN010000000
VERSION     DAZFRN010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.458"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            67..>657
                     /gene="acrS"
                     /locus_tag="ACHKUB_005378"
     CDS             67..>657
                     /gene="acrS"
                     /locus_tag="ACHKUB_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO6658217.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNV"
BASE COUNT          192 a          139 c          150 g          176 t
ORIGIN      
        1 tgatagatta accttcgcta ttttctcact ctgtgtcgaa tatatttatt tcctgaataa
       61 ttaatcatgg caaaaagaac caaagccgaa gctctgaaga cccggcaaga actgattgaa
      121 actgccatcg cccagtttgc gcagcatggc gtaagcaaga cgacactcaa cgacattgcc
      181 gacgctgcta acgttacgcg tggcgctatc tactggcact tcgaaaacaa gactcaactg
      241 tttaatgaga tgtggttgca acagccttca ttgcgagagt taatccagga tcacttgacg
      301 gctgggttag agcatgaccc atttcaacaa ttgcgtgaaa aattgattgt cggcttgcaa
      361 tatattgcca aaattccccg ccagcaggcg ttgctgaaaa tcttatatca caaatgtgaa
      421 tttaatgatg agatgctggc cgagggagtg atacgcgaaa agatgggctt caatccgcag
      481 actctccgcg aagtattgca ggcgtgtcag caacaaggtt gtgtagcaaa taacctcgat
      541 ttagatgttg tgatgattat tattaatggt gccttcagcg gaattgttca aaactggtta
      601 atgaatatgg cgggttatga tctttataaa caagcccccg ctctggtcga taacgta
//
LOCUS       DAZFRN010000455          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFRN010000455 DAZFRN010000000
VERSION     DAZFRN010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.459"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            70..>656
                     /locus_tag="ACHKUB_005379"
     CDS             70..>656
                     /locus_tag="ACHKUB_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6658218.1"
                     /translation="MKKPYRISDSHGLYLEISSSGSRLWYFRFRFNGKQNRISLGHYP
                     QTTLVDARRKRDAARRLLASGESPAEHRKSERATASKARKFQHIALGWHTSCLNLWSD
                     VYANKILVCLQRYVFPYIGDTNIAKIETFHLAEVIRNIDNKGVHDVAIRTRQYLTKIM
                     RYAIQKGIIYYNPAFDLEGVIPPANTRHYPALPLN"
BASE COUNT          213 a          147 c          123 g          173 t
ORIGIN      
        1 ctaacactta gtaaaaaaag agatactacc atgccgttaa atgactatat aattcttaat
       61 gcagctccaa tgaaaaaacc ataccgaatt tctgattcac atggtctata cctcgaaata
      121 tcaagtagtg gctcacgctt atggtatttt cgattccggt tcaatggtaa gcaaaatcgt
      181 atatcgcttg gacattaccc tcaaacgaca cttgtggatg ctcggaggaa acgagatgca
      241 gccagaagat tgctggcatc aggagaatcc cctgccgaac atagaaagtc cgaaagagct
      301 acagccagca aagcacgaaa atttcaacac attgctcttg gatggcacac cagttgttta
      361 aatctttggt cagatgtata tgctaataaa atccttgtat gtctccagcg gtatgttttc
      421 ccatatattg gggatacaaa tatagctaaa attgaaacct ttcatttggc ggaggtcatc
      481 agaaacatag ataacaaggg ggtacatgat gtcgctattc gaacacgtca atacctaaca
      541 aaaataatgc gttacgctat acagaaaggc attatatatt acaatcctgc ttttgatttg
      601 gagggcgtca tccccccggc aaatacccgt cactatcctg ccctccccct aaacca
//
LOCUS       DAZFRN010000456          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFRN010000456 DAZFRN010000000
VERSION     DAZFRN010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.460"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>653)
                     /locus_tag="ACHKUB_005380"
     CDS             complement(<1..>653)
                     /locus_tag="ACHKUB_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO6658219.1"
                     /translation="AAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPR
                     SATEQRKSELLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERW
                     IVDRYNIRHSLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAM
                     AVDTGGEAGVTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARR
                     "
BASE COUNT          142 a          214 c          156 g          141 t
ORIGIN      
        1 cggcgggcac ttctgccggt attatccggg aaggtacggt taatcagcct gtcacggcga
       61 agaccatccc ccttgaacag aaacaccctg ttgcccagtc cgtcactccg gcaacgacgc
      121 cagaaacgat aggcgttatc tgtcaccccg gcttcccctc ccgtatccac cgccatggcc
      181 atcagacgca tgcgcacatc cggatcagaa gccagcggcc atgttttatg gaacacatcc
      241 gtcagcaaca aatcccagtc ctccggatat gccgccggat caaccggcag actttcaccg
      301 ttgggactgc agcgcagtga atgccggatg ttgtagcgat caacaatcca gcgttccccc
      361 tgctctccgt atccggtgat ctgcacaaca aaacggcgat ttttaccgcc ctgtacgtca
      421 accgttgcct caataaaacg cacaccatcc ggcacagatc gccggggaaa cggctcggca
      481 cgctgttcaa gcagttcact tttacgctgt tccgtggctg aacggggcag atagggtcgt
      541 ccgatatcgg tgttcaccac cgctttcagg gtctcttcac tgccggttcg ctcatactct
      601 tcttctgccg ccagcagttt aaaaatcagt tgttcccagg tctgaaacgc cgc
//
LOCUS       DAZFRN010000457          645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFRN010000457 DAZFRN010000000
VERSION     DAZFRN010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.461"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
BASE COUNT          161 a           95 c          156 g          233 t
ORIGIN      
        1 cggtctgaat atttatcccg ttctttttta tttgtgcagt gatactgtcg gaagagaatt
       61 cagtaatgtg aggtgtcctg gtcaggataa tatctgctga gatattatta gttgagtgtg
      121 ttttactgag caaaagctgg cttttaattc ctgtactgaa acactgtcgc tcgtccgggg
      181 ttaactgaaa ctggatgatg atgtaaaatg aaggctgttt ttgtgtttgt cgaagaagtg
      241 ttattttaat ccattctcat cattggctgg cgattataca gaagtatttg aagaaaaaat
      301 ggtgcatttt atctgtgtca gcagggaaga agtattttat tgatgtatca gtattaaatg
      361 gtttttgtta tttataaaaa tgtcatcctg tccataaaat attgtactca gcattcagtg
      421 caggaactgt agtcttcttt attttattct tcttcaggag ggggggtgat tcctgaaggc
      481 gttattcccg gtgttctgtt tatgattctt cccggcagga aaccggtatc tgcaataccc
      541 gggatgatgc tttttgttcg tacgtggttg tgtaaaattt gaatagtgga tgtgctccga
      601 agggaaggag gggacacaca gattcatttt gtctttcttc agcag
//
LOCUS       DAZFRN010000458          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFRN010000458 DAZFRN010000000
VERSION     DAZFRN010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.462"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>642
                     /locus_tag="ACHKUB_005381"
     CDS             <1..>642
                     /locus_tag="ACHKUB_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658220.1"
                     /translation="SEGRRFVSFHHVDELRICASCGLTEVHHAPENHKPDPEWYCSSL
                     CRETETLCQEIYERPYNSFISDATANGLILMKLPETWSTNEKMFASGGQGHGFAAERG
                     NHIVDRVRLKNARILGDNNARNGADRLVSGTEIQTKYCSTAARSVGAAFDGQNGQYRY
                     MGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASRLIRRGHLTYT"
BASE COUNT          176 a          155 c          169 g          142 t
ORIGIN      
        1 tttcagaagg tcgccgtttt gtatcattcc atcacgtgga tgagcttcgt atctgtgcct
       61 cctgcgggct gacagaagta catcatgccc cggaaaatca taagccggat ccggaatggt
      121 actgctcctc tctttgtcgc gaaacagaaa cactgtgtca ggaaatttat gaacgccctt
      181 acaacagctt tatttccgat gcaacggcga atggtctgat tctcatgaaa ctgccggaaa
      241 cctggagtac aaatgagaaa atgtttgctt ccggagggca gggacatggg tttgccgctg
      301 aacggggaaa ccatattgtc gacagagtcc gtctgaaaaa cgcacggatc ctcggtgata
      361 ataatgccag aaatggagca gacagactgg tcagcggaac agaaatccag acgaaatatt
      421 gttcaactgc agcccgtagc gtcggtgcgg cattcgacgg acaaaacgga cagtatcgtt
      481 acatgggaaa taatggcccc atgcaactgg aagtcccccg tgatcagtat gccggcgctg
      541 ttgaaaccat gaggaataag atccgcgaag gtaaagtacc cggtgtaacc gatcccgcag
      601 aagcgtcccg gctgattcgt cggggacatc tgacttatac tc
//
LOCUS       DAZFRN010000459          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFRN010000459 DAZFRN010000000
VERSION     DAZFRN010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.463"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>628
                     /locus_tag="ACHKUB_005382"
     CDS             <1..>628
                     /locus_tag="ACHKUB_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO6658221.1"
                     /translation="FIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDS
                     LYLLKNTEGIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGP
                     LVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHD
                     LLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKL"
BASE COUNT          191 a          117 c          138 g          182 t
ORIGIN      
        1 ttcatcatta actatcaact ggtatcggaa cgcgcggtaa aacgtgccga tagccgcttt
       61 gaacttattc agaaaaacgt tggctatttc tttaaagata ttgaacgttc ggccctgaca
      121 ttaaaggact cactgtattt attaaaaaat acagagggga ttcaacgcgc cgtgattctg
      181 aaaatggaaa tgatgccatt tttagactcg gtgggactgg tacttgatga taataaatat
      241 tatctctttt cacggagggc gaatgataaa atcgttgttt atcatcagga acaagtaaat
      301 ggaccgcttg tcgacgagtc agggcgggtt atttttgccg attttaaccc atcgaaacga
      361 ccgtggtcgg tggcttcaga tgactctaac aacagctgga atccggcata caattgcttt
      421 gatcgtccgg gtaaaaaatg tatctctttt acgctacgta tcaacggcaa agatcacgat
      481 ttgttagcgg tagataaaat tcatgtcgat ttaaactggc ggtatctgaa cgagtatctt
      541 gatcaaatca gcgctaatga tgaagttcta tttttgaaac aaggtcatga gatcattgcc
      601 aagaatcaac tcgctcgtga aaaactga
//
LOCUS       DAZFRN010000460          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFRN010000460 DAZFRN010000000
VERSION     DAZFRN010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.464"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            8..571
                     /gene="fimA"
                     /locus_tag="ACHKUB_005383"
     CDS             8..571
                     /gene="fimA"
                     /locus_tag="ACHKUB_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6658222.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSVKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          154 a          124 c          162 g          184 t
ORIGIN      
        1 gagaattatg aaactcaaac atgttggtat gattgtcgtt tctgtgttgg cgatgtcgtc
       61 tgctgcggta agcgcagccg agggtgatga atcagtaacg accactgtta atggcggtgt
      121 tattcatttt aaaggtgaag tggtaaatgc cgcttgtgca attgattcag aatcaatgaa
      181 ccaaacggtt gagctgggtc aggttcgttc ttctcgcctg gctaaagcgg gtgacctcag
      241 ctccgccgtt ggcttcaata tcaagctgaa tgattgtgat accaatgttt ccagtaatgc
      301 agctgttgca ttcctgggta ctactgtcac cagtaatgac gatacgttag cgctgcagag
      361 ttcagcggca ggctctgccc aaaatgtcgg tattcaaatt ttggaccgta cgggtgaggt
      421 attaatactt gatggggcca cttttagtgt taaaaccgac ttgattgatg gcacgaatat
      481 actgccattc caggctcgtt atattgctct cgggcagtcc gtagctggta ctgcaaacgc
      541 tgatgcgacc ttcaaagttc aatatctata attcaatctt aaggtggcta tacgtcatct
      601 gaaattgaaa cagggtgtgt gtgg
//
LOCUS       DAZFRN010000461          618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFRN010000461 DAZFRN010000000
VERSION     DAZFRN010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.465"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>618
                     /locus_tag="ACHKUB_005384"
     CDS             <1..>618
                     /locus_tag="ACHKUB_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO6658223.1"
                     /translation="YQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRYV
                     AACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTARLRIKNTTEGCTHYVVSVYD
                     PNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHIINQSVAIIPALPKEQLL
                     MLKGSVDEITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRT"
BASE COUNT          206 a          120 c          122 g          170 t
ORIGIN      
        1 tatcagaatg ttgattacaa accccatcaa gatgattact cttcagctga aaaatttctc
       61 acgcacttca acaaaaaatg caaaaaccag actttggcgt tggtttccag ccgtcctgag
      121 gggcgttacg ttgctgcctg tggtgatttc gggctagtta tgaaggcata tttcgacaag
      181 atggaatcaa atggcatcag tgttatggca gccatattat tggtggataa ccatgctttg
      241 acggcgcggc taagaataaa gaacacaact gaaggatgta cccattacgt ggtttcggtt
      301 tatgatccta atgtcactaa cgataaaata agaattatga gcgaaagcaa agaggatatt
      361 aaacactatt ctctgatgga ttttatgaat gtagattata gcctcctgaa atggtcaaat
      421 gatcatatta ttaaccaatc tgttgcaata attccagcac ttccgaaaga acagctattg
      481 atgttaaaag gatctgtgga tgaaataacc cctccattat cacctgcaac gatgaatttg
      541 ctaatggcaa ttggtcagaa tcaccaactt acgcaactga tgattcaact ccagaaaatg
      601 ccagaactac atagaaca
//
LOCUS       DAZFRN010000462          601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFRN010000462 DAZFRN010000000
VERSION     DAZFRN010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.466"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(190..408)
                     /gene="ymgF"
                     /locus_tag="ACHKUB_005385"
     CDS             complement(190..408)
                     /gene="ymgF"
                     /locus_tag="ACHKUB_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO6658224.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
BASE COUNT          202 a          125 c          111 g          163 t
ORIGIN      
        1 taacaaataa ctcttcgaca tcaaaattgt taatagttgc gatgaggatt atttcggtga
       61 tatataaaaa caaccgtgat aacaccaggg ttgacaacta tttgcgcaat aatgcctttc
      121 cttcaatgat ccaggcgttc actgccagca acccattcag ccgcatactg acagtgagaa
      181 actgaaagat tattgctgat ccctgtggtt gacgagtctt tctcttaatc gattaaccag
      241 attgatcgcc agccaaacaa gggcattgag caaatttatc gtcacaagca cttctcccag
      301 cccggtactg ggttttgcaa taaggatgac caggagggtg atcagcataa atgcaatgga
      361 aaagatgata tagagagtga aataatccag attattacta ttgttcatgc tactaatggc
      421 aatcctgtca aaactttcaa acaattatag caaacaaaat aatgaacaat agtaacaaga
      481 ggcaacgagc taattagctg tctataatta caccgcacta tggccttact gatcaatttt
      541 cactcaacca caaccttcac cttcggttta gaaaggataa ttttgaccag gtcgatgtat
      601 g
//
LOCUS       DAZFRN010000463          595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFRN010000463 DAZFRN010000000
VERSION     DAZFRN010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.467"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(146..>595)
                     /locus_tag="ACHKUB_005386"
     CDS             complement(146..>595)
                     /locus_tag="ACHKUB_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6658225.1"
                     /translation="VPGTIGLLIRQFYYELTLTSCGKNLRVFWGAYIVYPDVMIGDNC
                     TIEEFCIISKCRLGNDVILAARVSIMSGSKHHDINDITKTFGESKAEYRTIHLGNNLW
                     IGTHAVIMNDIGDHTAIGAGSVVTRAIPEMVVAAGVPARIIKHRGKL"
BASE COUNT          219 a          130 c           84 g          162 t
ORIGIN      
        1 aagcctatta atataaaaaa accaaaccca ataataaaaa aattaacagg atagaaagga
       61 aataacccaa agagagaaaa cagagttaaa actggagaaa cgacaagtat gataaatata
      121 tatatttttg aaaatacttc tgacattata gcttcccacg atgttttatt attcgagcag
      181 gaacaccagc agcaacaacc atttcaggaa tcgccctcgt tacaacagat ccggcaccta
      241 tagccgtgtg atcgccaata tcattcataa tgacagcgtg tgtaccaatc cataggttgt
      301 ttcctaaatg gattgtgcga tactcagctt tgctttcacc aaaagtttta gtgatatcat
      361 ttatatcatg atgtttcgaa ccgctcatta ttgataccct agctgcaagt ataacatcat
      421 ttcctagcct acacttgctt ataatacaaa actcctcaat cgtacaatta tccccaatca
      481 taacgtcagg ataaacaata tatgctcccc aaaacactct taaatttttc ccacaagatg
      541 ttaaggtcag ttcatagtaa aactgcctaa tcaacagacc aatagttcca ggcac
//
LOCUS       DAZFRN010000464          591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFRN010000464 DAZFRN010000000
VERSION     DAZFRN010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.468"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            73..>591
                     /locus_tag="ACHKUB_005387"
     CDS             73..>591
                     /locus_tag="ACHKUB_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6658226.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVT"
BASE COUNT          174 a          115 c          138 g          164 t
ORIGIN      
        1 attattgtta ttattaagtc taatgtcatt taaaggcatt atcatattta cgaagagatc
       61 aggatgatga cgatgaaaaa aagtgtattg acggcgttta taactgtggt atgtgcaacg
      121 tccagcgtta tggcagctga tgataatgct atcacggatg gcaaagtaac atttaatggt
      181 aaagttattg ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg
      241 ccaaatgtca gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat
      301 gtaccaattg ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt
      361 agtggtacgg ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca
      421 gcgacgaatg ttgctctaca aatgtatctg ccagatggtt ctacatcggt aactcccggt
      481 accgaaacgt cgaatattca gttagcggat tctgctgagc aaacagtgac ctttaaagtt
      541 gattatatcg ctacggggaa agcgacttct ggtaatgtga atgcggtaac a
//
LOCUS       DAZFRN010000465          589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFRN010000465 DAZFRN010000000
VERSION     DAZFRN010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.469"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>589
                     /locus_tag="ACHKUB_005388"
     CDS             <1..>589
                     /locus_tag="ACHKUB_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658227.1"
                     /translation="LETVWMQISSTLKGALDINSLIIDYDNEDVQSYWEDVVLPNLND
                     NGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMIAEYAVSRNAII
                     SSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTLNDSLIEWKPSY
                     YQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNE"
BASE COUNT          207 a           86 c          122 g          174 t
ORIGIN      
        1 ttagaaacgg tttggatgca gatatcatcg actctcaaag gtgctctgga tatcaacagc
       61 ttgatcattg actacgataa tgaagatgtc caaagttatt gggaagacgt tgtattaccg
      121 aacttaaatg ataatggtaa taaagctctc acaagagatg aaacaatagc ctatattaaa
      181 gacagtaagc cttgtagaag aattaaaaga ttaatacatg atgatgctgt gttggaggat
      241 caaaaggata aaaacttcat tgcaaaaatg attgcagaat atgctgtgtc tagaaatgca
      301 attatttcta gcaacacatc tggcaatgtc attcggggtg atgaaagcgg agtaaaatgg
      361 attcaatgtg gtaatatctt tgtctcccta tttcataagg ttcaagatga tcatgaaaac
      421 gatggagata ggatttggca aactctcaat gattctctca ttgaatggaa accatcttat
      481 tatcagttaa taaaatctga aattcagaat gcaatcgaag ctgaggcttt atcttttgta
      541 aatcatttgg ctaacgatca ttacggtcaa gctgcgtggt taaatgaga
//
LOCUS       DAZFRN010000466          579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFRN010000466 DAZFRN010000000
VERSION     DAZFRN010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.470"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..450)
                     /locus_tag="ACHKUB_005389"
     CDS             complement(<1..450)
                     /locus_tag="ACHKUB_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO6658228.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRKLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFE"
BASE COUNT          175 a          111 c          105 g          188 t
ORIGIN      
        1 ttcgaataag taacgaacgc ctttcttgcc tgatttatag gtgagtattt tgtatccatc
       61 gtaatcatat ttacaggatg ttgtttcatt atttctatgg aattcaacaa tgccgtcctc
      121 aatgccaatc atctcgatat ctgttgcata acctttgtga taataatctt taatttcagc
      181 aaatgtttta gttttatctg catccgcttt cttctgaaat acagggtcaa gtttgccact
      241 ttgcagtaaa ggataaacgg attgccagac tccatcccag tcactgagct ttcggttttg
      301 tacattggca tcatcaaaaa caccattagc agctttttgt tcgacctctg ttaagggttt
      361 gccgtgtgag tgatgaccat gcgaaaaagc aggagcgcta acaataaaga cacctaaagc
      421 aacagccagt ttgtgaagac gaatcgccaa aacagtttcc tccagagtca ttgtatatga
      481 tatgtaatgt tatattgtaa catatgacaa aatgcaacaa ctcttaggag acaagagaat
      541 agttctctca ggtgtgatta tctatcttat tgaaataaa
//
LOCUS       DAZFRN010000467          577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFRN010000467 DAZFRN010000000
VERSION     DAZFRN010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.471"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            183..>577
                     /locus_tag="ACHKUB_005390"
     CDS             183..>577
                     /locus_tag="ACHKUB_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO6658229.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALR"
BASE COUNT          135 a          147 c          153 g          142 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca
      481 agactgatac cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag
      541 cctgggaagc gcctcacccg cttgaacgcg cgttgcg
//
LOCUS       DAZFRN010000468          563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFRN010000468 DAZFRN010000000
VERSION     DAZFRN010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.472"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            186..307
                     /locus_tag="ACHKUB_005391"
                     /pseudo
     CDS             186..307
                     /locus_tag="ACHKUB_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            335..563
                     /locus_tag="ACHKUB_005392"
                     /pseudo
     CDS             335..563
                     /locus_tag="ACHKUB_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
BASE COUNT          159 a          118 c          142 g          144 t
ORIGIN      
        1 aaaaatgaaa aggcaatatt aaaaaaaata aaaaaactca aacgtaatcc taaagctttt
       61 cttattgact ctaagcacag gtctttgaat ttgttagcca gatttctgtt ctaattttgg
      121 atagcagaat atgcaatgtt atttttcgaa tcctccataa ctgatatatg tgctgttagt
      181 ggagtacaag aataagctcg cgttagcagt ctgtcagtac tgcgacagcg ttgtctggga
      241 gggctttacc cggtcaaaac agcattattg tgcaccccat gtgacggatg aactgtgtgc
      301 tcagggggca ttttactgta aaaaccgtgc tgcggtcagc cgtaaccgtc aggtgggtga
      361 acgcaacagt cgcgccattg cttttcaggc acgtaccctg caggcgcatg agcgtcgtat
      421 gaacagtcag cagcgtcaga ttaatgagaa tcatgaggaa atgaagcggg cagcagccca
      481 gtcagcagcg ctggccggtc ttttccagcc atacagtgtg ggtaaattta acgccacagc
      541 ggctgtgggc gggtacagtg aca
//
LOCUS       DAZFRN010000469          563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFRN010000469 DAZFRN010000000
VERSION     DAZFRN010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.473"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            125..508
                     /gene="traM"
                     /locus_tag="ACHKUB_005393"
     CDS             125..508
                     /gene="traM"
                     /locus_tag="ACHKUB_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO6658230.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSELERFFQKNDEE"
BASE COUNT          183 a           73 c          128 g          179 t
ORIGIN      
        1 ccggatcgca tttttttata ggatgatcct agaggcgctg ctaggagtgt cttttttact
       61 tgtaaacact atatgcatat ttatagtgaa aagttcattg cttaataatg aaaggtttta
      121 tcttatggct agagtaattt tgtatatcag taatgatgtc tatgataaag taaatgcgat
      181 agttgaacag cgtcgtcagg agggggcaag agataaagat ataagtgttt cgggaacagc
      241 ttcaatgctt cttgaactgg ggcttcgtgt atatgaggct cagatggagc gtaaagagtc
      301 tgcatttaat cagactgagt ttaataaatt gcttcttgaa tgcgttgtaa aaacacagtc
      361 aacagttgcc aagattttag gtattgagtc actcagtcct catgtatcag gaaatccaaa
      421 gtttgaatat gcaaatatgg tcgaagatat cagggagaag gtgtcatctg agctggaaag
      481 atttttccaa aaaaatgatg aggaataaaa aagaattaaa agttgatttg cttcaaataa
      541 caggattttg atctggttca att
//
LOCUS       DAZFRN010000470          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFRN010000470 DAZFRN010000000
VERSION     DAZFRN010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.474"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            1..551
                     /locus_tag="ACHKUB_005394"
                     /pseudo
     CDS             1..551
                     /locus_tag="ACHKUB_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598008.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          144 a          136 c          117 g          154 t
ORIGIN      
        1 tttaccaacc ctgggtcact gcattatcgc caacgccccg acacgtcagt cctgagcaat
       61 tagaaaaaac agttcgttat cttacacaaa ctgtgcatcc acgtaatgcc gacaatattg
      121 ataatctgaa taggtccgta gagtatataa aagagttctt tgtcagtagc ggtgccagag
      181 ttaccgcgca gggcgtcccc gattggctat tacgactctg cgcctggtag ccaggattac
      241 ccttacccgc aatgtcctgg ctttatcccg atcgggggga ctttattgcc tggtcggcag
      301 aatacaggat atcaacgccg ttcgtcaggt aaaaacggca ttgttatcat cccgggattt
      361 atctgtttat tctatgaatg ccccatggtt tattcccggt attgatttct ctgaccacct
      421 gaattattgg caacacgata ttcccgccgt aatgattact gacaccgctt tttatcgtaa
      481 taaacaatac cacttgcccg gtgatacagc agacagattg aattatcaga aaatggctca
      541 gatggcgtta t
//
LOCUS       DAZFRN010000471          541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFRN010000471 DAZFRN010000000
VERSION     DAZFRN010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.476"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            21..>541
                     /locus_tag="ACHKUB_005395"
     CDS             21..>541
                     /locus_tag="ACHKUB_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658231.1"
                     /translation="MKMIFTGKVSGEKTVLTAGGRHTVKAQPGEQYGLIDEVTGLVPD
                     GVEADRSGDDLILRKKEEDTEVRIEGFWEECQPGETQCTAVFNIVGEDGQVTEAVLTQ
                     DGPVLDDITAGQSGTLSDDDRGGFIWLGGLAFGGGLAAMALAAGGGGSKHRHENDDSD
                     TTAPSSPALKAED"
BASE COUNT          142 a          101 c          174 g          124 t
ORIGIN      
        1 aattagccta aggattttat atgaaaatga tttttaccgg taaggtcagc ggagaaaaaa
       61 ccgtgctgac tgccggaggt cgtcatacag ttaaagcaca gcccggggag cagtatggac
      121 tgatcgatga ggtgaccggc ctagtgcctg acggtgttga ggcggatcgc tccggtgatg
      181 atttaattct gcgtaaaaag gaagaagata ctgaggtccg gatagaggga ttctgggaag
      241 aatgccagcc cggggaaaca cagtgtacgg cggtatttaa tattgttggg gaagatggac
      301 aggtcaccga agccgtactg actcaggatg gcccggtgct ggatgatatt actgccggac
      361 aatcgggaac cttgtcagat gatgatcgtg gcggttttat atggttgggc ggtctggcat
      421 ttggtggggg actggctgct atggctttag ctgcaggtgg cggtggaagc aaacatagac
      481 atgaaaatga tgactctgat actacagccc catcgtcacc agcactgaag gcagaggatg
      541 a
//
LOCUS       DAZFRN010000472          535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFRN010000472 DAZFRN010000000
VERSION     DAZFRN010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.477"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            18..>535
                     /locus_tag="ACHKUB_005396"
     CDS             18..>535
                     /locus_tag="ACHKUB_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6658232.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAG"
BASE COUNT          163 a           98 c          112 g          162 t
ORIGIN      
        1 tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt acgccgcttt
       61 tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct atggctgttt
      121 ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat gaagttcagt
      181 acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa aatgaagttg
      241 agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattt gctttaccag
      301 ccggtagtag caaaaccgta cgttttgttg cgatggaacc agagcaaaaa gagaaaaatt
      361 atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat aaaaaagatc
      421 tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt ccacctcagc
      481 aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca gggaa
//
LOCUS       DAZFRN010000473          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFRN010000473 DAZFRN010000000
VERSION     DAZFRN010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.478"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..256)
                     /locus_tag="ACHKUB_005397"
     CDS             complement(<1..256)
                     /locus_tag="ACHKUB_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6658233.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHML"
     gene            complement(434..505)
                     /locus_tag="ACHKUB_005398"
     CDS             complement(434..505)
                     /locus_tag="ACHKUB_005398"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658234.1"
                     /translation="MFIQDDPGMHFRRVIRNAEGQRR"
BASE COUNT          114 a          137 c          149 g          128 t
ORIGIN      
        1 gcagcatgtg ttttgtgtat ccccggcggc ccgggtcgcg cacacgggtc tggcaggcga
       61 aaaagggctg gaagccttcc cgctgcagac tttccagtac ggtgatggtg gggatgtacg
      121 tatagttctg gcttcgggat gtgtgtttct cttccccgaa aatccccggc acatggtgca
      181 tcagctcttc atgtgtcagc ggacggtcac ggcgtatctg gtttgcagca taaccaaaac
      241 gactgactaa tcgcatactt tgctccttat cggtggttaa gatttactgg tgcaataaat
      301 gaaaaagccg cgtctcccgg agaagacgcg gcctgatgga cgaaatcaat gacgtttatt
      361 gtctgagaag ccctccactg gcgagccacg cattaagctg ttttccggca tcagcttcag
      421 agttccggca gcgtcagcgt cgctgacctt ccgcgttgcg gataacccga cggaaatgca
      481 ttcccgggtc atcctggata aacacattgc ggtaagcggt tatgacag
//
LOCUS       DAZFRN010000474          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFRN010000474 DAZFRN010000000
VERSION     DAZFRN010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.479"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..401)
                     /locus_tag="ACHKUB_005399"
     CDS             complement(<1..401)
                     /locus_tag="ACHKUB_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658235.1"
                     /translation="MSDVNICTADLQSLIRMDPEFRGMVQRKLAADKNIFVKQLYEDL
                     DDAIQNLENDKHFYQDAKWGEDELTASIKNFLKGRFYDVEHDTQHGGHVDLLVKHQFG
                     KFEWIGEAKLWRGPKSIHNGWIQLTERYGTGT"
BASE COUNT          155 a          109 c           89 g          170 t
ORIGIN      
        1 gtacccgtgc cgtaacgttc ggttagctga atccagccat tatgaataga tttggggcct
       61 ctccataatt ttgcttcacc gatccattcg aattttccga attgatgttt aactaataaa
      121 tcaacgtgac cgccatgttg agtatcatgt tcaacatcat aaaacctacc tttcaggaaa
      181 tttttgatcg acgctgttag ctcgtcctct ccccacttag catcttgata gaagtgtttg
      241 tcattttcta aattctgaat agcgtcgtct aaatcttcat agagttgctt tacaaaaata
      301 tttttgtctg cagctaattt tctttgaacc attcccctaa attcaggatc cattctaatt
      361 aatgattgaa ggtctgcagt acagatgttt acatcactca tgcagactct ccaaaagact
      421 cgaaggagta aagtatggat aaagatactg gctaaaatta tcaaccaatt cgccagtttc
      481 tggatggtaa aaacttcctg tgtctaaagc ataggaaata aga
//
LOCUS       DAZFRN010000475          515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFRN010000475 DAZFRN010000000
VERSION     DAZFRN010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.480"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>515
                     /locus_tag="ACHKUB_005400"
     CDS             <1..>515
                     /locus_tag="ACHKUB_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFO6658236.1"
                     /translation="NACLKLYNLAHAATNSPLKPAGPDNSEDLLFKKLFSPSKLMTII
                     GDEIPLISEKQSLSKVLLNDENNELSDGTNFWDKNRQLTTDEIDCYLQKIAANAKNTE
                     VNYPTGLYVPYSTRTHLEDALNENIKSDPSWPKAVQLFPINTGGHWILVSLQKIVNEK
                     NNTQQIKCVIF"
BASE COUNT          183 a          111 c           84 g          137 t
ORIGIN      
        1 aaatgcttgt ttaaaattat ataatttggc acatgccgca accaactccc cccttaaacc
       61 agctggtccg gataattcag aggatctcct gttcaaaaag ctattctccc cttcgaaatt
      121 aatgacaatt atcggtgatg aaattcctct tatatctgaa aaacagtcgc tttcgaaggt
      181 gcttttaaat gatgagaata atgaactgag tgatggtaca aacttctggg ataaaaatcg
      241 tcaattaacc acagatgaaa tagattgcta tcttcagaag atcgccgcta atgcaaaaaa
      301 tactgaagtc aattatccta ctggtctcta cgtcccctac tccaccagaa ctcacctgga
      361 agacgctctc aatgaaaata ttaagagcga tccatcatgg ccgaaagcag tccagttatt
      421 ccccataaat actggcggac actggatatt agtttcgcta cagaaaatag taaatgaaaa
      481 aaataataca caacaaataa aatgcgtcat attca
//
LOCUS       DAZFRN010000476          509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFRN010000476 DAZFRN010000000
VERSION     DAZFRN010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.481"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>509)
                     /gene="tssI"
                     /locus_tag="ACHKUB_005401"
     CDS             complement(<1..>509)
                     /gene="tssI"
                     /locus_tag="ACHKUB_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO6658237.1"
                     /translation="VYDYPGRFKGAHGQNFARWQMDGWRNNAETARGMSRSPEIWPGR
                     RIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENHFAVIPADRTWRPQP
                     LLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPADQDSSCWIRVAQAWAG
                     TGFGHLAIPR"
BASE COUNT           76 a          185 c          137 g          111 t
ORIGIN      
        1 cgcggtatcg ccaggtggcc aaaaccggtg cctgcccacg cctgtgccac acggatccag
       61 cacgaactgt cttggtctgc cgggttataa cggtcccagt tgaacttcac ccgcacgcga
      121 ccatgttcgt cgcagaagat ttcctcgcct gccggtcctg tcacgacagc gctctgcggg
      181 ccgtcgacca gcggtttcag caacggctgt ggtcgccatg ttctgtctgc cgggatcacc
      241 gcaaaatggt tctccagcgc cgtccccgct ccctgccgtc ctggcaccgc ctgtggctgt
      301 tcgccgtgca gttcacttgc caccacctgc cattcccggt tcaggttcgc ctgcggatgc
      361 cccgtcagca caattcgtcg tcccggccat atctccggcg agcgactcat tccccgcgcg
      421 gtttctgcat tgtttcgcca gccgtccatc tgccagcggg caaagttctg cccgtgggca
      481 cccttgaaac gtccggggta gtcatacac
//
LOCUS       DAZFRN010000477          502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFRN010000477 DAZFRN010000000
VERSION     DAZFRN010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.482"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..226)
                     /locus_tag="ACHKUB_005402"
     CDS             complement(<1..226)
                     /locus_tag="ACHKUB_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO6658238.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNG"
     gene            complement(228..>502)
                     /locus_tag="ACHKUB_005403"
     CDS             complement(228..>502)
                     /locus_tag="ACHKUB_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6658239.1"
                     /translation="SNNRLIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGH
                     GVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQFNGVEF"
BASE COUNT          164 a          102 c           79 g          157 t
ORIGIN      
        1 aaccattgtc aacatcacaa atcatatttt tagattgaaa aaagctctca agagtagcgg
       61 ctcgtttaga tgagtcaatt ttgttgtagt ctaaatcaac ttcgttattc aacgccctga
      121 ttgattcaga atacgtcaga aaatcgtcat caatcatgac aacggaacga attgcatttt
      181 cgcagaaagt tttctggaca agagaattat aatttgccac tgtcatatta gaactccact
      241 ccattgaact ggatcacaaa attagcgcca tcttttatta aatagttatc gccttcatca
      301 ggttctgaat accatatttt atgatgtgca acagcaaggt tttctcgaca tagatacaga
      361 cctaccccat gtccatttgc tcttttgcta taaaatagtt caaatagtcg cgggatatca
      421 tcggtatcaa ttgccggacc agaatttgct atgataacca aagaattcac aaaaccaatc
      481 tttatgagcc tattatttga ca
//
LOCUS       DAZFRN010000478          502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFRN010000478 DAZFRN010000000
VERSION     DAZFRN010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.483"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>502
                     /locus_tag="ACHKUB_005404"
     CDS             <1..>502
                     /locus_tag="ACHKUB_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFO6658240.1"
                     /translation="VFNVVGENGQVTEAVLTQDGPVLENFTAGQSGTLSDDDRGGFIW
                     LGGLAFGGGLAAMALAAGGGGGSKHRHENHEADTTAPSAPALTAGDNGSVSVELPDDA
                     NKGDTVEITFVNENGDGQKVTLEKGDNGWTSDNSALIPDSQGDTATIAPDTVKDNSEV
                     TAVAKDP"
BASE COUNT          137 a          102 c          149 g          114 t
ORIGIN      
        1 gtatttaatg ttgtgggtga aaatggtcag gtaactgaag ctgtcctgac ccaggatggt
       61 cctgtactgg agaatttcac tgcaggacaa tccgggacct tgtcagatga tgatcgtggc
      121 ggttttatat ggttgggcgg tctggcattt ggtggggggc ttgctgctat ggctttggct
      181 gcaggtggtg gtggcggcag caagcacaga catgaaaacc atgaggcaga tacaacagca
      241 ccatcagcac cggcattaac ggccggagat aatggttcag tttctgttga actgccggat
      301 gatgcgaata aaggcgatac tgttgaaatc acctttgtaa atgaaaatgg tgatggacag
      361 aaagtcacac tggagaaagg ggacaacggc tggacatccg acaattctgc actgatcccg
      421 gacagccagg gtgatacagc cacaatcgcc ccagacactg ttaaagataa cagtgaagtc
      481 acagcagttg ctaaagatcc gg
//
LOCUS       DAZFRN010000479          500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFRN010000479 DAZFRN010000000
VERSION     DAZFRN010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.484"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>500
                     /locus_tag="ACHKUB_005405"
     CDS             <1..>500
                     /locus_tag="ACHKUB_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658241.1"
                     /translation="IDFSTASPTVRQALQKGTGAGNISALNKVMKGTLVTSLALVAVT
                     TGPDMIKMLRGRISGTQFIRNLAVASSGVAGGAVGSVAGGILFSPLGPFGALTGRVAG
                     GVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWLAGSFLLTGHEIENLNENLAR
                     VIDQNAL"
BASE COUNT          108 a          113 c          161 g          118 t
ORIGIN      
        1 attgattttt cgactgccag cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca
       61 ggaaatatca gtgccctgaa caaagtgatg aagggtacgc tggtgacatc tctggcactg
      121 gtagctgtca caaccggccc tgacatgatc aaaatgttgc ggggacggat ctccggtacg
      181 cagttcatca ggaatcttgc cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca
      241 gtggcgggcg gaatactgtt cagtccactg ggaccatttg gtgcactgac agggcgtgtg
      301 gctggcggtg ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg
      361 ctggttgaag aagatcgcgt caaaattctg gcaatgattc aggagcaggt gacatggctt
      421 gccggcagtt tcctgctgac cggacatgag attgaaaatc tgaacgagaa tctggcccgt
      481 gttatcgatc agaatgctct
//
LOCUS       DAZFRN010000480          489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFRN010000480 DAZFRN010000000
VERSION     DAZFRN010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.485"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>489
                     /locus_tag="ACHKUB_005406"
     CDS             <1..>489
                     /locus_tag="ACHKUB_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO6658242.1"
                     /translation="QELNRTETAREVQRPSIDAHLLWLEAELKRLEKQIKDLTDDDPD
                     MKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRYESGSSVRGA
                     SRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGAMMRKLAQVA
                     YGV"
BASE COUNT          122 a          112 c          162 g           93 t
ORIGIN      
        1 caggaactga atcgcactga aacggcgcgg gaagtccaga gaccgagcat tgatgctcac
       61 cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc agataaaaga cctgacagac
      121 gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa gcatcccggg tatcggagag
      181 aaaacatctg cggtattgct ggcttatatc ggtctgaagg accgcttcgc ccatgccaga
      241 cagttcgccg cttttgcggg tctgacacca cggcgttatg aatcaggtag cagtgtgaga
      301 ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc gcagggcgtt gtatatgccc
      361 gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt tccgcgaccg tctggcggct
      421 aatggcaaga aaggaaaggt gattctcggc gcgatgatgc gcaagctggc acaggtggcg
      481 tatggagtg
//
LOCUS       DAZFRN010000481          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFRN010000481 DAZFRN010000000
VERSION     DAZFRN010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.486"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>485
                     /locus_tag="ACHKUB_005407"
     rRNA            <1..>485
                     /locus_tag="ACHKUB_005407"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZFRN010000482          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFRN010000482 DAZFRN010000000
VERSION     DAZFRN010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.487"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>476
                     /locus_tag="ACHKUB_005408"
     rRNA            <1..>476
                     /locus_tag="ACHKUB_005408"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZFRN010000483          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFRN010000483 DAZFRN010000000
VERSION     DAZFRN010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.488"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..153)
                     /locus_tag="ACHKUB_005409"
     CDS             complement(<1..153)
                     /locus_tag="ACHKUB_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFO6658243.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSK"
     gene            complement(165..>459)
                     /locus_tag="ACHKUB_005410"
     CDS             complement(165..>459)
                     /locus_tag="ACHKUB_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658244.1"
                     /translation="SLFCSTELNSKSQSPALGLGHEFAHAQYYLLDKENFMALLSRTD
                     KKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
BASE COUNT          142 a          115 c           67 g          135 t
ORIGIN      
        1 cttgctaagc ggaactaacg tggtaacata cgatggaaga ttaattatct ctatcgtttt
       61 cacgctatct attccacaca attcattcac tcgccccatc cccagtaccg aaccagaaaa
      121 tagagacaat aaaaccatca aatatttcat cataaaaaac ctcactattc tggcgtaccc
      181 gttattttca tcgtctgcaa cggtccatcc acccgataaa aaggcagtcc actatgagct
      241 cctctggtac actcacctaa agttttcgcc gcacgcgatt caattattgt aataaccctt
      301 gcctcttctt tattctcata tttcttatct gttcggctta aaagagccat gaaattttcc
      361 ttatcaagaa ggtaatactg cgcatgagca aactcatgtc ccaaacctaa tgcaggcgat
      421 tgtgatttcg agtttaattc agttgaacaa aataaagat
//
LOCUS       DAZFRN010000484          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFRN010000484 DAZFRN010000000
VERSION     DAZFRN010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.489"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
BASE COUNT          131 a           88 c          118 g          120 t
ORIGIN      
        1 tctggaaagc agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta
       61 ctactttgat gttaagttac tgatagtgca acatggcatc tcaacgaacg aattataacc
      121 acctctggac agtgttgcaa ttaagataat gagatttata atcctgactg ttgggaagtt
      181 gcgccggatc gaacccattt caagtcctgg ctaccggacg ggtggtgccg caggtagtgc
      241 tctgatccgg agtgcaacat ggcgactcaa caaacgtaaa ccagtatcga caggcagtgt
      301 aagggtaagg tgctggtgtt gcgctcctta ataaggagag cggatggaca cgctgacaca
      361 aaagttaacc gtgctcattg ccgtactgga gctattagtg tctctgttac ggttgattga
      421 tttgttgaag taatgggcaa attgaaacgc aactaag
//
LOCUS       DAZFRN010000485          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFRN010000485 DAZFRN010000000
VERSION     DAZFRN010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.490"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>457
                     /locus_tag="ACHKUB_005411"
     CDS             <1..>457
                     /locus_tag="ACHKUB_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO6658245.1"
                     /translation="YIQLKGAFLSAIHHQIIQNQLDTETITISYEFILCQHLIANTEF
                     SYLALPENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLG
                     GDAIKGYDQILNEKTAGIAIATASILSTKRSNVDTYTEINSYLGKLRGQQ"
BASE COUNT          172 a           83 c           77 g          125 t
ORIGIN      
        1 ttatatacaa ctaaaaggcg cttttttatc ggctattcat catcagatca ttcaaaatca
       61 actggataca gaaacaataa ctattagtta tgaatttatc ctctgtcaac atcttatcgc
      121 aaataccgag ttcagctatt tggcactccc tgaaaattat aaccgtttgt ttttaccaaa
      181 ttcaaaaaac caaacaaata atcgtttcaa aacgttaaac agcaaagcta ttggcaggct
      241 acttgctgct ggtggcgtat acaatgggaa cattgaagga ttcagagata ctgcggaaaa
      301 actgggtgga gatgcaataa aaggctatga tcaaatacta aatgaaaaaa cagcgggcat
      361 agcgatagca acagcatcta ttctttcaac aaagcgttct aatgttgata catatacaga
      421 aataaatagt tacttaggca aacttagagg tcaacaa
//
LOCUS       DAZFRN010000486          450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFRN010000486 DAZFRN010000000
VERSION     DAZFRN010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.491"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..127)
                     /locus_tag="ACHKUB_005412"
     CDS             complement(<1..127)
                     /locus_tag="ACHKUB_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658246.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVS"
     gene            complement(383..>450)
                     /locus_tag="ACHKUB_005413"
     CDS             complement(383..>450)
                     /locus_tag="ACHKUB_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO6658247.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          130 a           91 c           90 g          139 t
ORIGIN      
        1 tggagacaat ttctgcaaac tttcttttta ccgtccaata atcaatattt ctattgtgat
       61 tgttaaccac tgcgtgcatt ggcgggattg aagggtgaaa aatatgtgca attgggaagc
      121 ctttcattga tgcctgcctt gtcgtcatga caaacaataa tagatgctta ttttcctcgc
      181 agggttcttt cgcactatca aaaaacgctc atttacccgg cgttacagta aacgaagcaa
      241 cattctgtgg cctacgacga tcaaaagctc tgtgaaaatt tcccttgttg tctttgtctg
      301 accttatcgc ggcgatcagc aattattcgt tttgtgatcc tcaacacaaa atgctattcg
      361 tcgcctatga ggggagaaga atttatggaa aacgattgca ttcacaaaat gacgtcctgt
      421 cagaagagaa aaagttgacg ttaatcaatt
//
LOCUS       DAZFRN010000487          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFRN010000487 DAZFRN010000000
VERSION     DAZFRN010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.492"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(321..>448)
                     /locus_tag="ACHKUB_005414"
     CDS             complement(321..>448)
                     /locus_tag="ACHKUB_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO6658248.1"
                     /translation="RLVNRNCYLDKYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          138 a           74 c           93 g          143 t
ORIGIN      
        1 acggttgtta gatctataca aaaagtatct tttacagtgg cttcgctatt ttaaaaagga
       61 attcatataa ggaaaaatat aattatgtta tttattggca gattgagttg acagggggca
      121 gaactctgag ggtaagaaaa gtcagcaaca acaatccact acatcaacag accagcaatc
      181 gcgtgccgag atacgctgga aatacccgaa gattgaactg gcggatggtg tgactgacat
      241 cagtcagtga tgactgttta gcaaactatg ttcgatcagt cagcatattt gctgactggt
      301 cgaattaatt aacaatgatg ttaactcact cttttgccgg atgctctatt tttgggttgt
      361 gagtgtttta tttctaattg tgtataagaa gcattgattc gtgcatcgcc atatttatct
      421 aaataacaat ttctgtttac cagtctat
//
LOCUS       DAZFRN010000488          446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFRN010000488 DAZFRN010000000
VERSION     DAZFRN010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.493"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            318..>446
                     /locus_tag="ACHKUB_005415"
     CDS             318..>446
                     /locus_tag="ACHKUB_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO6658249.1"
                     /translation="MANRKQQRARAERLHTRTEINRRLHRAASVTFIMQSNVLHRLN"
BASE COUNT          103 a          140 c          105 g           98 t
ORIGIN      
        1 ttctccatgc acacacaaaa aaaccgcttg ccagcgttcg cctggtcggg ttacagtttt
       61 cccgcacctc ataaaacggg tgccgggatt gcaacccccg ctgatcaaca aagcgcacaa
      121 ccgcgccagc ggtttttttg tgcgtaccgt atcgtcacat ctttttcgca ccgaaattat
      181 ggtggggcgt acggggccga cttcggtcgg gccgggttct ttgttgaccg gtggttgcaa
      241 ccccgtacgt ctcgccaccc agaattgcaa cctctggatg gtgagttttc agaacttacc
      301 aacaaagagg ccacaccatg gcaaaccgca agcaacagcg cgcccgcgct gagcgtcttc
      361 acacccggac tgaaatcaac cgcagacttc atcgggctgc atctgtcacc ttcatcatgc
      421 agtcaaacgt attacaccga cttaac
//
LOCUS       DAZFRN010000489          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFRN010000489 DAZFRN010000000
VERSION     DAZFRN010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.494"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>443
                     /locus_tag="ACHKUB_005416"
     CDS             <1..>443
                     /locus_tag="ACHKUB_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658250.1"
                     /translation="GRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAGLK
                     RVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKF
                     YYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGETVRYRYD"
BASE COUNT          103 a          114 c          170 g           56 t
ORIGIN      
        1 gggcgggtgg tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag
       61 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
      121 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
      181 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
      241 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
      301 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
      361 agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
      421 acagtacgct accgctacga tga
//
LOCUS       DAZFRN010000490          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFRN010000490 DAZFRN010000000
VERSION     DAZFRN010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.495"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     repeat_region   76..409
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=137..165
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaactc"
BASE COUNT          101 a          116 c          123 g           97 t
ORIGIN      
        1 gtgttacgtg gatatgttgc ttattacaag tactgctaat ataaaaactt gagaaagaga
       61 taacgggtta tatggtggtt tatccccgct ggcgcgggga actcgcctga cattgcagac
      121 gttacaaatt gagaggcggt ttatccccgc tggcgcgggg aactcctcaa gatagagcct
      181 ccgggtcgag ccgcgatcgg tttatccccg ctggcgcggg gaactcttga actgttgatg
      241 ttatcaaaat atcagctccg gtttatcccc gctggcgcgg ggaactcggc gaacaaccga
      301 cccctgttgg acccgacgac ggtttatccc cgctggcgcg gggaactcgc ggaaaacagc
      361 caaaataacg caattaccgc cggtttatcc ccgctggcgc ggggaactca gcagcaacaa
      421 tatttcccgc agtgctg
//
LOCUS       DAZFRN010000491          435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFRN010000491 DAZFRN010000000
VERSION     DAZFRN010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.496"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>435
                     /locus_tag="ACHKUB_005417"
     CDS             <1..>435
                     /locus_tag="ACHKUB_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO6658251.1"
                     /translation="RQQINTRTRVLKTTLGKLSTWTTNPVTEADLENPFSRMTESRLL
                     TDFTQTPQKGNPSWLASQADVESKKAALKAQELARYPRVDLTGSVTRDDRQIGVSLSW
                     DLFNRNASYGVSEKAAQIAAATGRLDSVARMIDETGRLSLIT"
BASE COUNT          115 a          108 c          122 g           90 t
ORIGIN      
        1 tccggcagca gataaatacc cgcacccgag tgctcaaaac cacgctggga aaactttcga
       61 cctggaccac aaatccggta accgaagccg atcttgaaaa tcctttttcc cggatgacag
      121 agtccagatt gttaactgat tttacgcaga cgcctcagaa agggaatccg tcgtggcttg
      181 ccagtcaggc tgatgtagag agtaaaaaag cggcgctgaa agcgcaggag cttgcccggt
      241 atccccgggt ggatttaacg ggatcagtga cccgggatga caggcagata ggtgtcagtc
      301 tgtcctggga cctgttcaac cgtaatgcca gttatggtgt cagtgaaaag gccgcgcaga
      361 tagctgcagc taccggacga ctggactctg ttgcccgaat gattgatgaa accggacgat
      421 tatcgctgat aacgg
//
LOCUS       DAZFRN010000492          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFRN010000492 DAZFRN010000000
VERSION     DAZFRN010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.497"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..90)
                     /locus_tag="ACHKUB_005418"
     CDS             complement(<1..90)
                     /locus_tag="ACHKUB_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658252.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDT"
     gene            108..299
                     /gene="yfgG"
                     /locus_tag="ACHKUB_005419"
     CDS             108..299
                     /gene="yfgG"
                     /locus_tag="ACHKUB_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO6658253.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          105 a          109 c           82 g          132 t
ORIGIN      
        1 tgtatccact gacgcttttt tacataacga agaattgacc attttgtcct gttgtgcctt
       61 aatgtaagta ccgtccacag cgtgggacat acttcaagga accttttgtg agtcaggcaa
      121 ccagtatgcg aaaacgacac cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca
      181 gctttatctt cttctttggc cgttttatct actcgtccgt cggtgcctgg cagcaccatc
      241 agagcaaaaa agaagctcag caatccacac tctccgtcga atcaccggta caacgttagc
      301 ggttaccttc tccactttca cagaacataa cggcacttcg ctgtcggatg cttttgctgt
      361 ttgggattat caaagcggca gatattcttt catcttaaat tttacgtctt tatcctgact
      421 gatgttta
//
LOCUS       DAZFRN010000493          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFRN010000493 DAZFRN010000000
VERSION     DAZFRN010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.498"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(189..>413)
                     /locus_tag="ACHKUB_005420"
     CDS             complement(189..>413)
                     /locus_tag="ACHKUB_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO6658254.1"
                     /translation="VNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFRWVTN
                     IHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
BASE COUNT          113 a           76 c           92 g          132 t
ORIGIN      
        1 aatgtatgtc aggtgcgatg aatcgtcatt gtattcccgg attaactatg tccacagccc
       61 tgacggggaa cttctctgcg ggagtgtccg ggaataatta aaaacgatgc acacagggtt
      121 tagcgcgtgc acgtattgca ttatgccaac gccccggtgc tgacacggaa gaaaccggac
      181 gttatgattt agcgtggaaa gatttgtgta gtgttctgaa tgctctcagt aaatagtaat
      241 gaattatcaa aggtatagta atatctttta tgttcatgga tatttgtaac ccatcggaaa
      301 actcctgctt tagcaagatt ttccctgtat tgctgaaatg tgatttctct tgatttcaac
      361 ctatcatagg acgtttctat aagatgcgtg tttcttgaga atttaacatt tac
//
LOCUS       DAZFRN010000494          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFRN010000494 DAZFRN010000000
VERSION     DAZFRN010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.499"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            22..>401
                     /locus_tag="ACHKUB_005421"
     CDS             22..>401
                     /locus_tag="ACHKUB_005421"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6658255.1"
                     /translation="MANDLNMLLLGRILQAIGAGAGPVISKAIAKESFPPLKLKRALS
                     DISSTSAIIPLIAPLAGALILDYYNWNVIFIVMGAFSILTILLSPKETNKQEKKTHQD
                     NVKFVTKDFICGTILVSLMLSSLF"
BASE COUNT          136 a           72 c           70 g          123 t
ORIGIN      
        1 attggcagta ttgtatgttt tatggccaat gatttaaata tgcttctttt gggtagaatt
       61 ctacaggcaa taggtgcagg tgccggacct gtaatatcaa aagccattgc taaagagtct
      121 tttccaccac taaagctaaa aagagcactt tctgacatat catcaacaag tgcgataata
      181 ccattaatag cacctctggc cggagcatta atacttgatt attataactg gaatgtaata
      241 tttattgtaa tgggtgcatt tagcattcta accatactcc tgtcacctaa agagactaat
      301 aaacaggaga aaaaaacaca tcaagataat gtaaaatttg tgactaaaga tttcatctgc
      361 gggacaatac tcgtttcact aatgctaagc tcactatttt g
//
LOCUS       DAZFRN010000495          400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFRN010000495 DAZFRN010000000
VERSION     DAZFRN010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.500"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>400)
                     /locus_tag="ACHKUB_005422"
     CDS             complement(<1..>400)
                     /locus_tag="ACHKUB_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO6658256.1"
                     /translation="VFTRGMPWFGAESDHGKRGDLPVNQFRKWSVSASFQRPVTDRVW
                     WLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRNELSWSLFSLPYV
                     GTVRAVAALDGGWLHSDSDDPYSSGTLWGAA"
BASE COUNT           89 a          135 c          103 g           73 t
ORIGIN      
        1 cagcagcacc ccacagcgtg ccggacgagt acgggtcatc gctgtcagag tgcagccagc
       61 cgccgtccag tgcagccact gcacggacag ttcccacata tggcagggag aacagagacc
      121 aggacagctc atttcgcaga taaccgccgt tattaccgga gatatactgc tccttaaagc
      181 cacgcactga actctcaccc ccgaggctca gttgttccac accatgaaga cggtccggtg
      241 accactgggc ataagcgctg gtcagccacc acaccctgtc cgtgacgggg cgctgaaaac
      301 tggcactcac cgaccatttc cggaactgat ttacgggcag gtctcccctt ttcccgtggt
      361 cgctttctgc gccgaaccag ggcatccccc gtgtgaatac
//
LOCUS       DAZFRN010000496          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFRN010000496 DAZFRN010000000
VERSION     DAZFRN010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.501"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>398)
                     /locus_tag="ACHKUB_005423"
     CDS             complement(<1..>398)
                     /locus_tag="ACHKUB_005423"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6658257.1"
                     /translation="DISELTKIIENYSAIITIRVIKQRTKTNASVTRNIGIKNSIGEI
                     ICFLDADDIWRENKLEVVAKVFDDFKNESTIVFHQSLRGTLNDINAGKGKIVPETGPA
                     TNNIVDYLMNEHGVIQTSTISINRKSAEIL"
BASE COUNT          121 a           77 c           61 g          139 t
ORIGIN      
        1 caaaatttca gcagactttc tatttatact aatagttgaa gtctggataa caccatgttc
       61 gttcatcaaa taatcaacga tattgttggt ggcaggacct gtctcaggta caatttttcc
      121 ttttccagca ttaatatcat tgagggtacc tctcaatgat tgatgaaaaa caatcgtcga
      181 ttcgtttttg aaatcgtcaa aaaccttagc aaccacctca agcttatttt ctctccagat
      241 atcatcagca tcaaggaaac aaattatttc accaattgag tttttgattc ctatattcct
      301 ggtgacagag gcattcgttt tagtgcgctg cttaataact cgaattgtaa taattgctga
      361 atagttttca atgattttag tcagctcact aatatcat
//
LOCUS       DAZFRN010000497          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFRN010000497 DAZFRN010000000
VERSION     DAZFRN010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.502"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..204)
                     /locus_tag="ACHKUB_005424"
     CDS             complement(<1..204)
                     /locus_tag="ACHKUB_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6658258.1"
                     /translation="MATTCSVILILESFDVCFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLR"
BASE COUNT          162 a           84 c           52 g           97 t
ORIGIN      
        1 ccttaagtta ttagctttca aatagcgact aataaactca gcgccaaaat ccgccactat
       61 cactctttca ggatatgtca ggaaaaaatc tctagagcta gagtcgacaa gtacagatga
      121 acctctctcc agaaacacac tctcttttcc gaaacaaaca tcaaaggact ccaaaatcaa
      181 tataactgaa catgtagttg ccatatcatc cacccaattt aactgaaacc aggatgaagt
      241 atatgcatat aaagttcact ttgccaaact tcagtataaa aaaccacata aaaaataggg
      301 tgtgataaaa aataccgtaa caataaagca aaggattata aattccgtta cagttacaaa
      361 tgatactcaa gaaacaattc cttaggaaaa actta
//
LOCUS       DAZFRN010000498          384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFRN010000498 DAZFRN010000000
VERSION     DAZFRN010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.503"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..283)
                     /locus_tag="ACHKUB_005425"
     rRNA            complement(<1..283)
                     /locus_tag="ACHKUB_005425"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           77 a          105 c           82 g          120 t
ORIGIN      
        1 ctgattcagg ctctgggctg ctccccgttc gctcgccgct actgggggaa tctcggttga
       61 tttcttttcc tcggggtact tagatgtttc agttcccccg gttcgcctca ttaacctatg
      121 gattcagtta atgatagtgt gacgaatcac actgggtttc cccattcgga aatcgccggt
      181 tataacggtt catatcacct taccgacgct tatcgcagat tagcacgtcc ttcatcgcct
      241 ctgactgcca gggcatccac cgtgtacgct tagtcgctta acctcacaac ccgaagatgt
      301 ttctttcgat tcatcatcgt gttgcgaaaa tttgagagac tcacgaacaa ctctcgttgt
      361 tcagtgtttc aattttcagc ttga
//
LOCUS       DAZFRN010000499          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFRN010000499 DAZFRN010000000
VERSION     DAZFRN010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.504"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..293)
                     /gene="tnpB"
                     /locus_tag="ACHKUB_005426"
     CDS             complement(<1..293)
                     /gene="tnpB"
                     /locus_tag="ACHKUB_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6658259.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLL"
BASE COUNT           82 a          116 c          106 g           79 t
ORIGIN      
        1 tccagcagca tcgccagctg cgcctgcgta aggaacactt tgccatcacg ggctgacggc
       61 caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc accggtggac
      121 cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg gccggacatg
      181 ggatcgtctt tcagcgccgt ttgtactttc gcagccaggc cgttgaagcc atttctcata
      241 tcggtgatac cggcaaccag ccaaattttg gtcccggaag gtaacgggat catcgcttca
      301 gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag cggagcgtcc
      361 cgtgccggaa cgttacctca cag
//
LOCUS       DAZFRN010000500          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFRN010000500 DAZFRN010000000
VERSION     DAZFRN010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.505"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>381
                     /locus_tag="ACHKUB_005427"
     CDS             <1..>381
                     /locus_tag="ACHKUB_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658260.1"
                     /translation="PGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQ
                     GLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLY
                     KDLIGRTKAALKKNPKNVLFAVVW"
BASE COUNT           88 a           95 c          118 g           80 t
ORIGIN      
        1 caccgggcgg tgtcgcctgt aaatataacg acatcattcc ggcggaccat tgtctgcatg
       61 atgtgcagga catgagccgt cttaaccatc cgaaagcgga cctgtcaaag gggcagtacg
      121 gtaccgtggg gcaggggctg catatcgcca aaaaactgct gccgtttata ccggcgaatg
      181 cgggcattct gctggttccg tgctgtcgtg gtggctctgc gttcaccacc ggagctgatg
      241 gcacatacag tgacgcgagt ggtgcctcgg agaattcaac ccgctggggt gtggacaagc
      301 cgctgtataa ggaccttatc ggtcgaacaa aagcagcact gaagaagaac ccgaaaaatg
      361 tgctgtttgc cgtggtgtgg a
//
LOCUS       DAZFRN010000501          379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFRN010000501 DAZFRN010000000
VERSION     DAZFRN010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.506"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>379)
                     /locus_tag="ACHKUB_005428"
     CDS             complement(<1..>379)
                     /locus_tag="ACHKUB_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658261.1"
                     /translation="TGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLP
                     GYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSF
                     TYDDKYRGRMVAHRHTGRPEIRYR"
BASE COUNT           66 a          128 c          115 g           70 t
ORIGIN      
        1 aacggtaacg gatttccggc cggcccgtgt gacggtgcgc caccatccgg ccccggtatt
       61 tatcatcgta agtaaagctg cgcacctgtt tgccactacg gtcatacacc accgccagtt
      121 cgccgcgtgg cgtccagcca tagcgcacca gcggcgcggc aggtaaattc tccgggtatt
      181 ccgggtcgtg cgtcagccac acggcagaca gacggatgcc gttgtcccgg ccatattcgg
      241 tgtaacccgg cagggtatca ggaaaagcgg acggttccgt cccgccggaa atggcctgct
      301 gccgggcttc ttctgcccgc tgcgcctgcg tggtcagtac cagccggaag tgacgcccgg
      361 caccatccgt cacgccggt
//
LOCUS       DAZFRN010000502          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFRN010000502 DAZFRN010000000
VERSION     DAZFRN010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.507"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..323)
                     /locus_tag="ACHKUB_005429"
     CDS             complement(<1..323)
                     /locus_tag="ACHKUB_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658262.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RDINE"
BASE COUNT          119 a           79 c           55 g          125 t
ORIGIN      
        1 ttctcattaa tatctcttat ccgaaactca ctgccataaa gattattaat tatcactcta
       61 tatttaacat tgtcctgcag atacgtatca aaactaagtg ctggcgctcc tggctttgag
      121 gcatcatagt taatgttaga catataggca atcatacgat tgaagttcga ctctgctatt
      181 tcatcgcagt cacattgacc cgttgatata aagaaagtga gtgccgccat ggtagactca
      241 agcctttctt taaaagagtc gaaagccagg cttttaatat ttaaatcacc ataattactc
      301 tgtattaagt ttgtagaaaa catctcccgc cccatattgt tcacaaaatt attatctcat
      361 ttaaatctaa gtcactta
//
LOCUS       DAZFRN010000503          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFRN010000503 DAZFRN010000000
VERSION     DAZFRN010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.508"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            33..263
                     /locus_tag="ACHKUB_005430"
     CDS             33..263
                     /locus_tag="ACHKUB_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO6658263.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWYYPNSP
                     KNDKTALHPLHYWLHEGSEQKKNPFPIWFYPR"
BASE COUNT          100 a           87 c           81 g          110 t
ORIGIN      
        1 ttttttctgg taaggaaaat cagagttacg ctatgtggat tttgaacgta tttcaggtgc
       61 ataaatctgt cagaatctac atagcgcatt tttcagtttt caattgtcac ttacatccat
      121 ccattgatag atcattagcg tcctggtatt accccaattc tcccaaaaac gataaaacag
      181 ccctacaccc gcttcattac tggcttcatg aaggatctga gcaaaaaaag aatccttttc
      241 caatttggtt ttacccgcga taatggtaag cgtacagcga gggccgtatt gacggggatg
      301 tgttattcag ctggcagtgc tatgcgccac ggaagcagtt cgctgacccg gttgaccggc
      361 cagtctgcta tgacgcca
//
LOCUS       DAZFRN010000504          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFRN010000504 DAZFRN010000000
VERSION     DAZFRN010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.509"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>376
                     /locus_tag="ACHKUB_005431"
     CDS             <1..>376
                     /locus_tag="ACHKUB_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6658264.1"
                     /translation="YALWPGDVDAGNIDGSIHRWAKNEVIDNDREDWKRISASMRKQP
                     DALRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGRNQSATDTLSSP
                     VPETDAVETAIPDNEKTECKVEV"
BASE COUNT          115 a           94 c           93 g           74 t
ORIGIN      
        1 tacgctctct ggcctggtga tgtggatgcc ggaaacattg acggcagcat ccatcgctgg
       61 gcaaaaaatg aagttatcga caacgaccgc gaagactgga agcgtatctc ggcatcaatg
      121 cgcaaacagc ctgatgccct tcgctacgac cgccagacta tttttggcct tgtccgtgaa
      181 cgtccgatcg acattcacaa agaccctgtg gcactgaaca aatacattac tgaatacctg
      241 actacaaagg gcgtgtttga agatgaagga agaaatcaga gcgcaactga tactctctcg
      301 tcgccagtac cagaaactga tgcagtggaa acggcaattc cggacaacga aaaaaccgaa
      361 tgcaaagtgg aagtcg
//
LOCUS       DAZFRN010000505          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFRN010000505 DAZFRN010000000
VERSION     DAZFRN010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.511"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>375
                     /locus_tag="ACHKUB_005432"
     CDS             <1..>375
                     /locus_tag="ACHKUB_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO6658265.1"
                     /translation="YAIRLLSGPLNGCEYEILNGRLLVIIGNDVSLGRSDAFSELPEN
                     TIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLNQQIEVLGLKFAVKEK
                     NEVWQYSLPGIIENNIISTKQH"
BASE COUNT          127 a           57 c           75 g          116 t
ORIGIN      
        1 cttatgccat tcgtttgttg agcggaccat tgaatggatg cgagtatgaa atacttaatg
       61 ggcgtctact tgttatcatt ggtaatgatg tttcgttagg aagaagtgat gctttttctg
      121 agttaccaga aaacacaata gtcgttcctt atggcgagct tacaggtagt tttgagataa
      181 taattactac cgaccctgat ctggtagtaa cattcagaga attaacagca caagaacctg
      241 aagatagaac gttaacattg aatcagcaaa tagaagtttt aggtctcaaa tttgctgtaa
      301 aagaaaaaaa tgaagtttgg cagtattcat tgccaggcat tattgaaaat aacattattt
      361 ctacaaaaca acatt
//
LOCUS       DAZFRN010000506          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFRN010000506 DAZFRN010000000
VERSION     DAZFRN010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.512"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            35..>371
                     /locus_tag="ACHKUB_005433"
     CDS             35..>371
                     /locus_tag="ACHKUB_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO6658266.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSSKSFPASCNNVENASQ
                     LHEVWQKGAD"
BASE COUNT          113 a           79 c           91 g           88 t
ORIGIN      
        1 aacaatccgc tatgctttga aaaaggagaa agaaatgatg aaaaagttta tcgccccctt
       61 gttggcttta ctggttagcg gatgtcagat tgatccttat actcacgcgc caaccttgac
      121 cagcaccgac tggtatgatg ttggtatgga agatgcgata tcgggcagcg ccataaaaga
      181 tgacgatgca tttagcgatt cacaggcgga tcgcggtcta taccttaaag gatatgccga
      241 aggacaaaag aaaacttgcc agaccgattt tacttatgcc cggggacttt ccagtaaaag
      301 ctttcctgcc agctgcaaca atgttgaaaa tgccagccaa ctgcatgaag tatggcaaaa
      361 aggggctgat g
//
LOCUS       DAZFRN010000507          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFRN010000507 DAZFRN010000000
VERSION     DAZFRN010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.513"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            1..363
                     /locus_tag="ACHKUB_005434"
                     /pseudo
     CDS             1..363
                     /locus_tag="ACHKUB_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
BASE COUNT          119 a           50 c           79 g          115 t
ORIGIN      
        1 atcaaatgta aaaataatgg gtggaaatca tcctttgaat cgttttacta ctcacatgat
       61 gacttataac ggtgaatttg ataagtttgc tatgagtgaa tttgaacgta gctggacatt
      121 gaaaccattc attaccaaac ctgaaaatcc gattatagga aatgatgtct ggataggaaa
      181 tgatgttgta ttgaaaggtg gcatcgcaat aggtgatggt gctgttattg ctgccaattc
      241 cgttgttacc aaagatgtgc cccatatgcc atagttgcag gagttcctgc aaaaataatc
      301 agatttaggt ttgattcaaa tgttattgat gaactgttga gaataaaatg gtggaattat
      361 aac
//
LOCUS       DAZFRN010000508          354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFRN010000508 DAZFRN010000000
VERSION     DAZFRN010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.514"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            239..354
                     /locus_tag="ACHKUB_005435"
                     /pseudo
     CDS             239..354
                     /locus_tag="ACHKUB_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           78 a          105 c           67 g          104 t
ORIGIN      
        1 tgatgatctt tccctaatct cacataggat cagatctcgt ctgatcgttc cccctgcgct
       61 cactaccatg gctctttacc acagcagccg gggttgtttg ggaccgactt ccgacagcag
      121 atcccggtgg gcctgccacc atctcttcac aagcttatgc ttattacatg caaaacgtcc
      181 cgttttgcat ctttgtgcct acggcacacc gatgatcttt ccctgtgatc tttccctgaa
      241 tctgcccctg agaacctgaa tcaaacgtct gactcgcaga accatctgtt tctcgcgctc
      301 tgtagaactt catgaaaaag tcatcggtgc ttttatcgaa aaatacatgt tcta
//
LOCUS       DAZFRN010000509          350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFRN010000509 DAZFRN010000000
VERSION     DAZFRN010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.515"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>350
                     /locus_tag="ACHKUB_005436"
     CDS             <1..>350
                     /locus_tag="ACHKUB_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658267.1"
                     /translation="PGETQCTAVFNVVGENGQVTEAVLTQDGPVLENFTAGQSGTLAE
                     GINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEGEQKPVQPEGGQEAGKTDVL
                     STGSTEAGSGSSSD"
BASE COUNT           84 a           69 c          121 g           76 t
ORIGIN      
        1 ccgggtgaaa cacagtgtac agccgtgttt aatgttgtgg gtgaaaatgg tcaggttact
       61 gaagctgtcc tgacccagga tggtcctgta ctggagaatt tcactgcagg gcaaagtggc
      121 acactggctg aaggtatcaa taacgggatt atctgggggg ggattgcttt tggtgcaggg
      181 ctggcggcta ttgcactggc tgccggaggc ggtggtggaa gcagtcgtca taagcgtgca
      241 actgaaggtg agcagaagcc ggttcagcct gaaggcggtc aggaggctgg aaaaacagat
      301 gtattatcca caggctcaac tgaggcggga agtggaagct cttccgacca
//
LOCUS       DAZFRN010000510          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFRN010000510 DAZFRN010000000
VERSION     DAZFRN010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.516"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(26..178)
                     /gene="hokA"
                     /locus_tag="ACHKUB_005437"
     CDS             complement(26..178)
                     /gene="hokA"
                     /locus_tag="ACHKUB_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO6658268.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
BASE COUNT          100 a           81 c           67 g          101 t
ORIGIN      
        1 gggaataact tcccgccgaa gactcttact cttttaaatt gcaggctaaa aatgccgcca
       61 gctcataact cccctgttta atatgcaatt cacacagtga atctcttatc atccaggtga
      121 aaaataaaag cgtgaaacaa atcactatta aagaaagtaa tctatatttc tgcggcattc
      181 ccagccccct gtgttgattt ccaacgagta agaggctaac cttatggtgt tcaggcatag
      241 ggtagcctca ctttgattta tagtcaggtg gggcttttct ctgtctgcct ttcggtgaat
      301 acctgagaca aacagtctca agcacccgtg gctattctag cttaataag
//
LOCUS       DAZFRN010000511          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFRN010000511 DAZFRN010000000
VERSION     DAZFRN010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.517"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>348
                     /locus_tag="ACHKUB_005438"
     CDS             <1..>348
                     /locus_tag="ACHKUB_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFO6658269.1"
                     /translation="DAPVLSIPEVTDGYANADELKDGLQAEVTLPAGTAEGAVITLTV
                     TRPDKTTENVTHTVTKDEVAAGKVSMDIPKDAVIDGQNSVSVTLTQGSNPAKPGNVVD
                     FAADTQIPGDTDGD"
BASE COUNT           88 a           82 c          107 g           71 t
ORIGIN      
        1 gatgccccgg ttctgagcat tcctgaagtg actgacggtt atgccaatgc tgatgagctg
       61 aaagacggtt tgcaggcaga ggtcacgttg ccggcaggca cggcagaagg tgcagttatt
      121 acacttaccg tgacccgtcc ggacaaaacc acggagaacg tgacacatac cgtgactaaa
      181 gatgaagtgg ctgccggtaa agtatccatg gatattccga aggatgcggt gattgacggg
      241 cagaacagcg tcagtgtcac tctgactcag ggcagtaacc cggcgaaacc cgggaatgtg
      301 gttgattttg ctgccgatac ccagatcccg ggggataccg atggtgat
//
LOCUS       DAZFRN010000512          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFRN010000512 DAZFRN010000000
VERSION     DAZFRN010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.518"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>345
                     /locus_tag="ACHKUB_005439"
     CDS             <1..>345
                     /locus_tag="ACHKUB_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6658270.1"
                     /translation="CRPLIENGSIEPRYVDAIYHSHDTIGPYYVVGPGIAMPHARPEE
                     GANKLSLALTLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAKLFDNEKDIQ
                     AILTAKTTQDIL"
BASE COUNT           90 a           85 c           83 g           87 t
ORIGIN      
        1 catgccgacc gttgattgaa aacggttcta tcgaaccgcg ttatgttgat gcgatctatc
       61 actcgcacga cactatcggt ccgtactatg tggttggccc tggtattgcg atgcctcatg
      121 cgcgcccgga agagggggct aacaagctgt cattagcact gaccctgatc ccttcaggtg
      181 tgaattttga tgccgatgaa aacgatccag tgaaattact gattgtgctg gctgcaaccg
      241 acagtaccag ccacattgaa gcgatttctc aactcgccaa gttattcgat aacgaaaaag
      301 acatacaggc tattttgacg gccaaaacga cgcaggacat tttgt
//
LOCUS       DAZFRN010000513          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFRN010000513 DAZFRN010000000
VERSION     DAZFRN010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.519"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>344
                     /locus_tag="ACHKUB_005440"
     CDS             <1..>344
                     /locus_tag="ACHKUB_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6658271.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZFRN010000514          337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFRN010000514 DAZFRN010000000
VERSION     DAZFRN010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.520"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..331)
                     /locus_tag="ACHKUB_005441"
     CDS             complement(<1..331)
                     /locus_tag="ACHKUB_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6658272.1"
                     /translation="MNTAAEAIRALSLQVPGFRGQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGL
                     TATTMLFS"
BASE COUNT           71 a          122 c           86 g           58 t
ORIGIN      
        1 gtgagaacag catcgtggtg gcagtcagcc ctccggcact cagggctgcg ccccatgccg
       61 ccagtgttgc accaccagtg aaaaatgacc cgacaatcgc tgcaaccccc agcaccacct
      121 gaaaaacacc atttcccccg gctccggcca gtcgcggcac aatgtggatg accgctccct
      181 cacccagttg ttcgtgaaga cgggcgtaca ccgcctccgg tgcggtatcc tcaccggcaa
      241 tacgtatctg ataccagcct tcgttcatct gaccgcggaa tcccggcacc tgcagcgaca
      301 gggcacggat ggcctccgct gccgtgttca catacag
//
LOCUS       DAZFRN010000515          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFRN010000515 DAZFRN010000000
VERSION     DAZFRN010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.521"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>335)
                     /locus_tag="ACHKUB_005442"
     CDS             complement(<1..>335)
                     /locus_tag="ACHKUB_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO6658273.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIA"
BASE COUNT           78 a           96 c           91 g           70 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg ccccagtttg
      301 gcgatttcat ccgccagata agcggcaacc tggta
//
LOCUS       DAZFRN010000516          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFRN010000516 DAZFRN010000000
VERSION     DAZFRN010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.522"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>331
                     /locus_tag="ACHKUB_005443"
     CDS             <1..>331
                     /locus_tag="ACHKUB_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO6658274.1"
                     /translation="DGVNAEELKDGVQTEVTVPKGSAAGDTLTLTVTKPDGTTDTVEH
                     TLTADEVAAGKADVTIPADKVTADGQYSVTAEITDPAGNTSGQGQPADFAVDTVAPSA
                     PVLKAED"
BASE COUNT           88 a           83 c          110 g           50 t
ORIGIN      
        1 cagatggtgt gaatgccgaa gagctgaagg atggtgtaca gaccgaagtc acggtaccga
       61 aaggcagcgc tgccggtgat accctgacgc tgacggtcac caaaccggac ggcacgacgg
      121 acacggtgga gcacacgctg accgcagatg aagtggctgc aggtaaagct gatgtgacca
      181 ttccggcgga taaagtgacg gcagatggtc agtattctgt aaccgcggaa atcacagacc
      241 cggcaggtaa caccagtggc cagggccagc cggctgactt cgcagtggat acagtggcgc
      301 cgtcggcacc ggtattaaaa gcagaggatg a
//
LOCUS       DAZFRN010000517          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFRN010000517 DAZFRN010000000
VERSION     DAZFRN010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.523"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>327)
                     /locus_tag="ACHKUB_005444"
     CDS             complement(<1..>327)
                     /locus_tag="ACHKUB_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658275.1"
                     /translation="VRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLR
                     VTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLLV
                     VVMKKST"
BASE COUNT           79 a           80 c           73 g           95 t
ORIGIN      
        1 gtggattttt tcatcaccac aaccagcaga tcgctgatac tggttgttgg tgtccagtta
       61 ttcgctcctg atgaagatac ggtgaatgtc agtgtcagcg tcccctgtcc ggcaggcata
      121 tctataactg aggaaaatac gccctgagca tccgtcgtgg actgattaaa aatctcctga
      181 ccattgcggg tcactcttaa ccggcaggtt gaataccagt atgactgttg gttattactg
      241 ttgaaattct catgcttacc accgcggaat aacactggcg gtatcatgac ctgccggtca
      301 aatttctgat catcactgat tcttacc
//
LOCUS       DAZFRN010000518          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFRN010000518 DAZFRN010000000
VERSION     DAZFRN010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.525"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>322
                     /locus_tag="ACHKUB_005445"
     CDS             <1..>322
                     /locus_tag="ACHKUB_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6658276.1"
                     /translation="EITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKN
                     GIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFL
                     KRLFA"
BASE COUNT           89 a           79 c           94 g           60 t
ORIGIN      
        1 atgaaatcac gcagacggtc agtaaaaccc tggaagacca gagcgccacc atacagcaga
       61 tacagcgggt gcagacagac acaaataacg acctggctgc gctgtacatg ctgaaggtgc
      121 agaaaacaaa aaacggcatt ccgtatgttg ccggtattgg tgcggggatt gaggatgctg
      181 atggccagcc cctgagcaat atactgctgc aggcggaccg tatcgcgatg attaacccgg
      241 agaacggcaa caccacgccg ctgtttgtgg cgcaggggaa tcagctgttc atgaacgatg
      301 tgttcctgaa gcgactgttt gc
//
LOCUS       DAZFRN010000519          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFRN010000519 DAZFRN010000000
VERSION     DAZFRN010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.526"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            45..>322
                     /locus_tag="ACHKUB_005446"
     CDS             45..>322
                     /locus_tag="ACHKUB_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HFO6658277.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGP"
BASE COUNT           65 a           84 c           95 g           78 t
ORIGIN      
        1 taccggcttt tttattcacc ctcactctga ttaaggaaat gctgatgaaa cgacatctga
       61 atacctgcta caggctggta tggaatcaca ttacgggcgc tttcgtggtt gcctccgaac
      121 tggcccgcgc acggggtaaa cgtggcggtg tggcggttgc actgtctctt gccgcggtca
      181 cgtcactccc ggtgctggct gctgacatcg ttgtgcaccc gggtgaaaca gtgaatggcg
      241 gaacactggt aaaccatgac aaccagtttg tatccggaac agctgatggc gtgactgtca
      301 gtaccgggct tgagctgggg cc
//
LOCUS       DAZFRN010000520          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFRN010000520 DAZFRN010000000
VERSION     DAZFRN010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.527"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>320)
                     /locus_tag="ACHKUB_005447"
     CDS             complement(<1..>320)
                     /locus_tag="ACHKUB_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO6658278.1"
                     /translation="FVSGGRSSDFSVSHDARNFAAGVSLPYGYTLVDYTYSWSDYLST
                     IDNRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGSSR
                     KLTS"
BASE COUNT           79 a           94 c           81 g           66 t
ORIGIN      
        1 aatgaagtga gtttacggct gctgccctga agcagaacat catccagata attgtgaata
       61 atgcggtgct gcagacctcc ggtcagtgct gtcttcatgt ccccgttacg gaacaggaca
      121 tgcgacagtc ccagccggtg agtctgcagg tctcccgtgg aacgccaccg ccagccccgg
      181 ttatcaatgg tgctgaggta gtcactccat gaatacgtgt aatccaccag ggtatagcca
      241 tacggcagac tgacaccggc ggcaaaattc ctcgcatcat gtgacaccga aaagtcactg
      301 ctccgtcccc cgctgacaaa
//
LOCUS       DAZFRN010000521          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFRN010000521 DAZFRN010000000
VERSION     DAZFRN010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.528"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>314
                     /locus_tag="ACHKUB_005448"
     CDS             <1..>314
                     /locus_tag="ACHKUB_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO6658279.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZFRN010000522          312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFRN010000522 DAZFRN010000000
VERSION     DAZFRN010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.529"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>312)
                     /locus_tag="ACHKUB_005449"
     CDS             complement(<1..>312)
                     /locus_tag="ACHKUB_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658280.1"
                     /translation="AGVSFISMMILFRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWF
                     QFSVYVMRKYYRHILVRGKETETISERLIREADTREYELWRPVRRFFSILFLIVLLVC
                     IH"
BASE COUNT          113 a           65 c           59 g           75 t
ORIGIN      
        1 atgaatgcaa actaatagaa caatgagaaa aagaatggag aaaaagcgac ggaccggacg
       61 ccatagttca tattctcgtg tatcagcctc acgtatcaac ctttctgaaa tggtttcggt
      121 ttcctttcct cgcaccagaa tatgcctgta atatttcctc atgacatata ccgaaaactg
      181 aaaccagatt aatatggatg gacacacaac acataaactt ctgatgatat cacccgggaa
      241 agtatcttgg gcgggtaaaa gaaagtcata tactctgaat aaaatcatca tcgagataaa
      301 tgagacgcca gc
//
LOCUS       DAZFRN010000523          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFRN010000523 DAZFRN010000000
VERSION     DAZFRN010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.530"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>301)
                     /locus_tag="ACHKUB_005450"
     CDS             complement(<1..>301)
                     /locus_tag="ACHKUB_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6658281.1"
                     /translation="VERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIR
                     PGERVGIIGSIGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLL"
BASE COUNT           82 a           93 c           61 g           65 t
ORIGIN      
        1 tccagaagaa acactttatc ccggacaaaa caggggtcca gttgctgcat cgcaaccccg
       61 tcaagagtaa tttgtcccct gttctgacgg tacagcccca ccatcatttt cagcagcgtc
      121 gatttaccac aacctattga gccaataata cccacccgct ctccgggccg gatacagagc
      181 gacagaccgc acaatgcgtc gggtaatccg ggcttataag aaaaggagac atcactgaaa
      241 cgaaactctc cctggatatc cttcactgtc agataatgtg tctgactttc ccgctccacc
      301 g
//
LOCUS       DAZFRN010000524          300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFRN010000524 DAZFRN010000000
VERSION     DAZFRN010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.531"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>300
                     /locus_tag="ACHKUB_005451"
     CDS             <1..>300
                     /locus_tag="ACHKUB_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658282.1"
                     /translation="KEAEAALTQAKADNKITQQEHDDLAAKNDAVTAAKADAAKAVEG
                     LPAGDAKDGLNGRLAKVDGIDVPAVDENGNGKPDAEEAAEAVNAATAKVAEAEAK"
BASE COUNT           91 a           62 c          113 g           34 t
ORIGIN      
        1 aaaaagaagc agaagcggcg ctgactcagg cgaaggctga caataagatc acccagcagg
       61 agcatgatga tcttgcggcg aagaacgatg cggtaacggc ggcgaaagca gatgcggcga
      121 aagcggtgga aggtctgccg gccggagatg cgaaggacgg tctgaacggg cgtctggcta
      181 aagttgacgg tatcgatgta ccggcagtgg atgaaaacgg caacggcaag ccggatgcag
      241 aggaagcagc agaggcggtg aatgcagcga cagccaaggt agcagaagcg gaagcgaagg
//
LOCUS       DAZFRN010000525          296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFRN010000525 DAZFRN010000000
VERSION     DAZFRN010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.532"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>296)
                     /locus_tag="ACHKUB_005452"
     CDS             complement(<1..>296)
                     /locus_tag="ACHKUB_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6658283.1"
                     /translation="TTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDFV
                     AAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIAS"
BASE COUNT           56 a           99 c           81 g           60 t
ORIGIN      
        1 gacgcaatcc gggaatttcg tggcgttccg gtgatactgc cgtccgccgc cgcccgttca
       61 ccttcacgaa gccagatccc ctggttattc agttcacgct tctgttccgg agcaatcagg
      121 ccgcggcagt gcggacacat caggcgggcc gcctgcccgg cagccacaaa atcagggtta
      181 ttccggtaac cggtcatgtt atccatcacc ggctgaaaat attccccgca gtgcggacac
      241 ggccagtacc accggcggcg gtctccccgg ttatacagtg acaggatccc cgttgt
//
LOCUS       DAZFRN010000526          288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFRN010000526 DAZFRN010000000
VERSION     DAZFRN010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.533"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>288
                     /locus_tag="ACHKUB_005453"
     CDS             <1..>288
                     /locus_tag="ACHKUB_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658284.1"
                     /translation="ADLKTAIDGVSSSMQGAGFALKDSDDKEVKQSLGQAIKVSGDSN
                     ITTTAITNGDKGLKISLNNDLTVGDKAPGSIKVNGADGKEAVSLNGQDGTIK"
BASE COUNT           87 a           56 c           85 g           60 t
ORIGIN      
        1 gcagacctga aaacggcaat tgatggtgtg tcctccagta tgcagggtgc aggtttcgca
       61 ctgaaagact ctgatgataa ggaagtgaaa cagtctcttg ggcaggcgat taaagtatcc
      121 ggggacagca atatcaccac cacagccatc accaatgggg ataaagggtt aaaaatatcc
      181 ctgaataatg acctgactgt gggggataag gctcccggca gcatcaaagt taacggggct
      241 gatggtaagg aggctgtgag tctgaatggt caggatggca cgattaaa
//
LOCUS       DAZFRN010000527          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFRN010000527 DAZFRN010000000
VERSION     DAZFRN010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.534"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(95..>287)
                     /locus_tag="ACHKUB_005454"
     CDS             complement(95..>287)
                     /locus_tag="ACHKUB_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658285.1"
                     /translation="KQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLILTQ
                     LVMAGLADYQFGCYRRLQS"
BASE COUNT           70 a           80 c           67 g           70 t
ORIGIN      
        1 ctgaggtttg cttgtgatcc agacgcatga gcagatggct ggtgagtgcc cctaacaccc
       61 accagccgcc catttaccac aaataaaaaa gccttcatga ctgaaggcgt ctgtaacaac
      121 cgaactgata gtctgccaga cccgccataa caagctgggt cagtattaac tggcagcgtt
      181 cgcgtgaaag gtaagtattc tgcgcaatct ccccgactgt cgccggttcg gtgacgctta
      241 attcattaaa caccactctg gcggtttcgg tcatatcctg ctgtttc
//
LOCUS       DAZFRN010000528          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFRN010000528 DAZFRN010000000
VERSION     DAZFRN010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.535"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..195
                     /locus_tag="ACHKUB_005455"
     CDS             <1..195
                     /locus_tag="ACHKUB_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO6658286.1"
                     /translation="FGDSNATRTTDAFLETECVENVATTEIIKATEESNGHRVSLPLS
                     VFNPQDYHPLLITVSGKNVN"
BASE COUNT           80 a           70 c           64 g           68 t
ORIGIN      
        1 tttggtgaca gtaatgccac gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa
       61 aatgtcgcca caaccgaaat catcaaagca actgaagaaa gtaacggcca tcgtgtcagt
      121 ctgccgttat cggttttcaa tccccaggac taccatccac tgctgattac cgtttccgga
      181 aaaaacgtta actgatccct gaagtcagtt tcatgcttca gcctctgtca caacgcgtgg
      241 cagaggcttt ttatttatca ggagaaaaca gatatgccgg gt
//
LOCUS       DAZFRN010000529          279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFRN010000529 DAZFRN010000000
VERSION     DAZFRN010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.536"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..201
                     /locus_tag="ACHKUB_005456"
     CDS             <1..201
                     /locus_tag="ACHKUB_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO6658287.1"
                     /translation="FGDSNATRTTDAFLETECVENVATTEIIKATEESNGHRVSLPLS
                     VFDPQDYHPLLITVSGKMLTDP"
BASE COUNT           79 a           69 c           64 g           67 t
ORIGIN      
        1 tttggtgaca gtaatgccac gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa
       61 aatgtcgcca caaccgaaat cattaaagca actgaagaaa gtaacggcca tcgtgtcagt
      121 ctgccgttat cggttttcga tccccaggac taccatccac tgctgattac cgtttccgga
      181 aaaatgttaa ctgatccctg aagtcagttt catgcttcag cctctgccac aacgtatggc
      241 agaggctatt tatcaggaga aaccagatat gccgggtta
//
LOCUS       DAZFRN010000530          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFRN010000530 DAZFRN010000000
VERSION     DAZFRN010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.537"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
BASE COUNT           83 a           56 c           55 g           84 t
ORIGIN      
        1 gtgacaatta cgcatagttt cccgataaag acgcgacagc tacatcccgc ataaggcatt
       61 tttctcttta tctttgtacg gtacttcatg gaacagagtt tttgaccttg cgaatcgtga
      121 tgtctgttgg gggcaacttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca
      181 gaaatcaaaa aacccggtca cttttttaca aggtaaccgg gtaaaaataa tttttatttt
      241 ttaactgttt tgagactcat agagatgtct caaaacta
//
LOCUS       DAZFRN010000531          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFRN010000531 DAZFRN010000000
VERSION     DAZFRN010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.538"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            32..>270
                     /locus_tag="ACHKUB_005457"
     CDS             32..>270
                     /locus_tag="ACHKUB_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO6658288.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVT"
BASE COUNT           74 a           70 c           61 g           65 t
ORIGIN      
        1 gtcatcgtca tgactcagag aggtaaatac catgattcac ctgttcaaaa cctgcatgat
       61 taccgccttc attctggggt taacgtggtc tgccccactc cgggcacagg atcaacgtta
      121 catcagtata cgcaatacag atacgatatg gctcccggga aatatttgtg cttaccagtt
      181 ccggctggat aatggcggaa acgatgaagg atttggcccc ctcaccatca ctctgcaact
      241 caaagacaaa tatggtcaga cgctggtgac
//
LOCUS       DAZFRN010000532          269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFRN010000532 DAZFRN010000000
VERSION     DAZFRN010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.539"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            17..>269
                     /locus_tag="ACHKUB_005458"
     CDS             17..>269
                     /locus_tag="ACHKUB_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6658289.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCP"
BASE COUNT           64 a           78 c           66 g           61 t
ORIGIN      
        1 caggaaaacc ttcagtatga aaacattacc cgatacacac gtacgggagg catcgagctg
       61 cccgtctccc gtcaccatct ggcagactct gctctcccga ctactgggcc agcattacgg
      121 ccttacactg aatgacacac cgttcgccga tgaacgtgtg attgagcagc atattgaagc
      181 tggcatttca ctgtgtgatg cggtgaattt tctcgtggaa aaatacgcgc tggtgcgtac
      241 cgaccagccg ggattcagca cttgtcccc
//
LOCUS       DAZFRN010000533          264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFRN010000533 DAZFRN010000000
VERSION     DAZFRN010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.540"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>264
                     /locus_tag="ACHKUB_005459"
     CDS             <1..>264
                     /locus_tag="ACHKUB_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HFO6658290.1"
                     /translation="SVATQTFDGIAQNMAAMLTGSEQNWRGFTRSVLSMMTEILLKQA
                     MVGIVGRIGSAIGGAFGGGASASTGTAIEAAAANFHFATGGFTG"
BASE COUNT           49 a           66 c           93 g           56 t
ORIGIN      
        1 aagtgtggcc acgcagacct ttgacggtat tgcacagaat atggcggcga tgctgaccgg
       61 cagcgaacag aactggcggg gattcacccg ttcggtgctg tccatgatga cagaaatcct
      121 gcttaaacag gccatggtgg gcattgtcgg gcgtatcggc agcgccattg gtggtgcttt
      181 cggtggtggg gcgtctgcct ccacggggac ggccattgag gctgcggcgg cgaacttcca
      241 tttcgcgacc ggaggattta cggg
//
LOCUS       DAZFRN010000534          263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFRN010000534 DAZFRN010000000
VERSION     DAZFRN010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.541"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(<1..>263)
                     /locus_tag="ACHKUB_005460"
     CDS             complement(<1..>263)
                     /locus_tag="ACHKUB_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6658291.1"
                     /translation="KVPGALVPNQFALGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTD
                     ATNLNAMYHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPV"
BASE COUNT           57 a           83 c           63 g           60 t
ORIGIN      
        1 accggcgtca cctgaatgct gttgtttccg gcaaactcaa aagccagtgt gtgccatccg
       61 ttatcaaaga ccccgaacgt atccagcttc gcattctgct tcttgtggta catcgcgttc
      121 aggttcgtcg catccgtctg caggaagaag gacatcagca tgtcgttgcc ttcctctgac
      181 agcgtcactc cctccggcag ggacgacaac tgccagtaaa tacccagggc aaactgattc
      241 ggcaccagtg cgcccggcac ctt
//
LOCUS       DAZFRN010000535          262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFRN010000535 DAZFRN010000000
VERSION     DAZFRN010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.542"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>262
                     /locus_tag="ACHKUB_005461"
     CDS             <1..>262
                     /locus_tag="ACHKUB_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658292.1"
                     /translation="KILSGSNLTLTAQVLANTGSGLVQAATLLLDVVNTVNGGRVLAT
                     GSADVKGTTLNNTGTLQGADLLVNYHTFSNSGTLLGTSGLGVK"
BASE COUNT           58 a           78 c           79 g           47 t
ORIGIN      
        1 aaaatcctct ccggcagtaa cctcacgctg acggcgcagg tgctggcgaa caccggcagc
       61 ggactggtac aggctgccac cctgctgctg gatgtggtga atactgtcaa cggcggacgc
      121 gtacttgcca ccggcagtgc cgacgttaaa ggaaccacgc tgaataatac cggtacgctt
      181 cagggtgcgg acctgctggt gaattaccac acattcagca acagcggtac cctgctggga
      241 acctccgggc ttggcgtcaa gg
//
LOCUS       DAZFRN010000536          259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFRN010000536 DAZFRN010000000
VERSION     DAZFRN010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.denovo.543"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            <1..>259
                     /locus_tag="ACHKUB_005462"
     CDS             <1..>259
                     /locus_tag="ACHKUB_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO6658293.1"
                     /translation="FGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQ
                     TRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAE"
BASE COUNT           58 a           80 c           74 g           47 t
ORIGIN      
        1 acttcggcag tcgttccgaa aaagtctccc gccgtatcgc acaaatggaa gccgatctga
       61 accggcttca gaaagagagc gatacgctga ctggtagggt gtatgacccg gcagtacagc
      121 gtccgttgcg tcagacccgc acccgtaagc cgttccctga atcactaccc cgtgacgaaa
      181 agcgactgtt gcctgcggcg ccgtgctgcc cgaactgcgg cggttcactg agctatctgg
      241 gcgaggatac cgccgaaca
//
LOCUS       DAZFRN010000537        17282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.guided.101, whole genome shotgun sequence.
ACCESSION   DAZFRN010000537 DAZFRN010000000
VERSION     DAZFRN010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.guided.101"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(50..595)
                     /gene="orn"
                     /locus_tag="ACHKUB_005463"
     CDS             complement(50..595)
                     /gene="orn"
                     /locus_tag="ACHKUB_005463"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO6658294.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            690..1742
                     /gene="rsgA"
                     /locus_tag="ACHKUB_005464"
     CDS             690..1742
                     /gene="rsgA"
                     /locus_tag="ACHKUB_005464"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO6658295.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            1839..2807
                     /gene="asd"
                     /locus_tag="ACHKUB_005465"
     CDS             1839..2807
                     /gene="asd"
                     /locus_tag="ACHKUB_005465"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO6658296.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGEKDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            2829..6152
                     /gene="mscM"
                     /locus_tag="ACHKUB_005466"
     CDS             2829..6152
                     /gene="mscM"
                     /locus_tag="ACHKUB_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO6658297.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6303..7805)
                     /gene="yjeM"
                     /locus_tag="ACHKUB_005467"
     CDS             complement(6303..7805)
                     /gene="yjeM"
                     /locus_tag="ACHKUB_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO6658298.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTLHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8024..9001)
                     /gene="epmA"
                     /locus_tag="ACHKUB_005468"
     CDS             complement(8024..9001)
                     /gene="epmA"
                     /locus_tag="ACHKUB_005468"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6658299.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9326..11134
                     /gene="frdA"
                     /locus_tag="ACHKUB_005469"
     CDS             9326..11134
                     /gene="frdA"
                     /locus_tag="ACHKUB_005469"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6658300.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11127..11861
                     /gene="frdB"
                     /locus_tag="ACHKUB_005470"
     CDS             11127..11861
                     /gene="frdB"
                     /locus_tag="ACHKUB_005470"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6658301.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            11872..12267
                     /gene="frdC"
                     /locus_tag="ACHKUB_005471"
     CDS             11872..12267
                     /gene="frdC"
                     /locus_tag="ACHKUB_005471"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6658302.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12278..12637
                     /gene="frdD"
                     /locus_tag="ACHKUB_005472"
     CDS             12278..12637
                     /gene="frdD"
                     /locus_tag="ACHKUB_005472"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6658303.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            12700..13833
                     /locus_tag="ACHKUB_005473"
     CDS             12700..13833
                     /locus_tag="ACHKUB_005473"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO6658304.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPTWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            13922..14455
                     /gene="blc"
                     /locus_tag="ACHKUB_005474"
     CDS             13922..14455
                     /gene="blc"
                     /locus_tag="ACHKUB_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6658305.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14452..14769)
                     /gene="sugE"
                     /locus_tag="ACHKUB_005475"
     CDS             complement(14452..14769)
                     /gene="sugE"
                     /locus_tag="ACHKUB_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6658306.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(14944..15090)
                     /gene="ecnB"
                     /locus_tag="ACHKUB_005476"
     CDS             complement(14944..15090)
                     /gene="ecnB"
                     /locus_tag="ACHKUB_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6658307.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15201..15326)
                     /gene="ecnA"
                     /locus_tag="ACHKUB_005477"
     CDS             complement(15201..15326)
                     /gene="ecnA"
                     /locus_tag="ACHKUB_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6658308.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15378..15944)
                     /gene="efp"
                     /locus_tag="ACHKUB_005478"
     CDS             complement(15378..15944)
                     /gene="efp"
                     /locus_tag="ACHKUB_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6658309.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            15986..17014
                     /gene="epmB"
                     /locus_tag="ACHKUB_005479"
     CDS             15986..17014
                     /gene="epmB"
                     /locus_tag="ACHKUB_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6658310.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4302 a         4543 c         4474 g         3963 t
ORIGIN      
        1 atcacgccgc cgcaagtgga ttccggcgct tacgtgacca ggaaaaattt tacagcttga
       61 taaaatgctc gcggtagtaa gccagttccg ccaccgattc acggatatca tccatcgcct
      121 gatgcgtccc ctgcttggta aaaccatcca gaatttccgg cttccagcgg cgcgccagct
      181 ctttcagggt gctgacatcg agataacggt agtggaagta ggcttccagc tccggcatgt
      241 atttaaacag gaaacgacgg tcctgtccga tgctgttacc gcaaatcggc gattttcccg
      301 caggcaccca ctgttttaag aattcgagcg ttgccagttc agcttcccga tcgcccatcg
      361 tgctcgcttt cacgcgctct accagcccgc tggcggtatg ggtgcgcacg ttccagtcat
      421 ccatcagcgc cagctgttca tcggactgat gtactgcaat ggtcggccct tctgccagaa
      481 tattcaggtt ggcatcggtc accagcgtgg caatctcaat aatgcgatcg cgctcgggat
      541 ccagaccggt catctcaaga tcgatccaaa tcaggttgtt ttcattggca ctcatgctat
      601 tttccaccca ttcacagtga ctatattgat ccacaattgc gtgtatcata gatgttttgc
      661 ccatcagggg cgaccaggag tctgtacgat tgagtaaaaa taaactctcc aaaggccagc
      721 agcgccgcgt gaacgccaat caccagcgtc gtcttaaaac gtctaaggag aagcccgact
      781 acgacgacaa tctgtttggc gagcctgatg aaggtatcgt catcagccgc tttggtatgc
      841 acgctgatgt ggaatccgcc gatggcgacg ttcaccgctg caatattcgc cgtaccatcc
      901 gttcgctggt gaccggcgac cgcgtagtct ggcgtccggg taaaccggcg gcggaaggcg
      961 taaatgtcaa agggatcgtg gaagcggtgc atgagcgcac ctcagtgttg acgcgtccgg
     1021 atttctacga cggcgtgaaa cctattgccg ccaacatcga ccagattgtc attgtctccg
     1081 ccattttgcc ggagctgtcg ctcaatatta tcgaccgtta cctggtggcc tgcgaaacct
     1141 tgcagattga gccgattatt gtgctcaata agatagacct gctggacgac gaaggcatgg
     1201 cgttcgtcaa cgagcagatg gatatctacc gcaacattgg ttatcgcgta ttgatggttt
     1261 ccagccatac tcaggatggg ctaaaaccgc tggaagaggc gttgaccggg cgcatcagca
     1321 tttttgccgg ccagtctggc gtcggcaaat ccagcctgct gaacgcgttg ctggggctgc
     1381 aaaaagagat cctgaccaac gatgtctctg acaactcggg tctcggtcag cacaccacga
     1441 ccgccgcacg actgtatcac ttcccgcacg gcggtgatgt gattgattcc cctggtgtgc
     1501 gtgagttcgg cctctggcac ctggagccgg aacaaatcac tcagggcttt gtcgaattcc
     1561 atgactattt aggtctgtgt aaatatcgcg attgcaaaca cgataccgat ccgggctgcg
     1621 ctatccggga agcggttgag gaaggaaaaa tcgcggaaac ccgtttcgaa aactatcacc
     1681 gtattctgga aagcatggcg caggtaaaaa cgcgtaaaaa cttttctgat acggatgact
     1741 gacaattaag ctaaccgtcg ttagaatcgt cccctttttt tcaggatccg gcatgtaggc
     1801 cggatcagga acgacaaaac aatggcctgg aggctacctt gttaaattca tttaaacttt
     1861 cgctacagta cattctgccg aaactatggc ttactcgcct ggcgggttgg ggcgcaagca
     1921 agcgggcagg atggctgaca aaactggtta tcgatctgtt cgttaaatac tacaaggtcg
     1981 acatgaaaga ggcgcaaaag ccggacaccg ccagctaccg cacctttaac gaattctttg
     2041 tccgtccgct gcgtgacgaa gtacgcccaa tcgataccga tccgaatgtc ctggtcatgc
     2101 ctgccgatgg cgttatcagc cagttgggta aaatcgaaga agataaaatc ctgcaagcca
     2161 aaggccacaa ctacagcctc gaagccctgc tggcaggcaa ctatctgatg gcggacctgt
     2221 tccgcaacgg tacgtttgtg accacttacc tctccccgcg tgactaccat cgcgtacaca
     2281 tgccgtgcaa cggtattctg cgtgagatga tctacgtacc gggcgatctc ttctccgtta
     2341 accatctcac ggctcagaac gtgccgaatc tgtttgcccg taacgaacgc gtgatttgcc
     2401 ttttcgatac cgaatttggc ccgatggcgc agattctggt cggggcgacg attgtaggca
     2461 gcattgagac ggtctgggcg ggcaccatta cgccgccgcg cgaaggtatc atcaagcgct
     2521 ggacctggcc tgccggggaa aaagacggtt ctgtggcact gctgaaaggc caggaaatgg
     2581 ggcgctttaa actcggctct accgttatca acctgtttgc accaggtaaa gtgaacctgg
     2641 ttgagcaact ggaaagcctg tctgttacga aaattggtca gccgctggca gtatctaccg
     2701 aaacctttgt tacgccagac gctgaaccgg ccccgcttcc tgctgaagag atcgaagcag
     2761 aacacgacgc cagcccattg gttgacgata aaaaagacca ggtctaatcc atcaaaggaa
     2821 acgctgacgt gcgcctgatt atcacttttc tgatggcctg gtgcctcagt tggggggcgt
     2881 acgccgcgac ggcccccgat agcaaacaaa tctcacaaga actggagcag gcaaaagcgg
     2941 cgaaacccgc gcagccggaa gtcgtagagg cgctccagtc cgccttaaat gcgcttgagg
     3001 aacgaaaagg ttcccttgag cgcatcaagc aatatcaaga agtcattgat aattacccga
     3061 aactttccgc tactctgcgc gcacaattaa acaatatgcg tgacgaaccg cgcagcgtgt
     3121 cgccggggat gtctaccgac gcgctgaatc aggaaattct ccaggtcagc agccagttgc
     3181 tggataaaag ccgtcaggcc cagcaagagc aggagcgcgc ccgcgagatt gccgattcgc
     3241 tgaatcaact gccgcaacag caaaccgacg cccgccgtca gttaaatgag atcgagcgcc
     3301 gcctgggaac gcttactggc aataccccgc tcaatcaggc acaaaatttc gcgttgcagt
     3361 ctgactccgc acgcctgaaa gcgctcgttg atgaactgga actggcgcag ctgtctgcca
     3421 ataaccgtca ggaattagcg cgcttacgct cagagctggc ggaaaaagag agccagcaac
     3481 tggatgcgta tttgcaggcc ttacgtaatc agttgaacag ccaacgtcag cttgaggcgg
     3541 agcgggcgct ggaaagtacc gaattgctgg cagaaaacag cgccgatttg ccgaaagaca
     3601 tcgtcgcgca attcaaaatt aaccgcgaac tatcggcggc actgaatcaa caggcgcagc
     3661 ggatggatct cgttgcctcg caacagcgcc aggccaccag ccagacgtta caggttcggc
     3721 aggcgctgaa tacgctgcgt gaacagtcgc aatggctggg atcgtccaac ctgctcggcg
     3781 aagcgttgcg ggcgcaggtg gcacggctgc cggaaatgcc gaaaccacaa cagcttgata
     3841 ccgaaatggc gcagttgcgt gtgcaacggt tacgttatga ggatctgctt aataaacagc
     3901 cgctgctacg gcaaattcat caggccgacg gtcagccgct gaccgccgag caaaaccgta
     3961 ttctggaagc acaactacgc actcagcgtg agttgctgaa ctcattgttg cagggtggcg
     4021 acacgctact attggaactg accaagctga aagtctccaa cgggcaactg gaggatgcgc
     4081 tgaaagaggt gaacgaagca acgcaccgct atctgttctg gacctctgac gtgcgcccga
     4141 tgaccatcgc ctggccgctg gaaatcgccc aggatctgcg tcgtctcatt tcgctggaca
     4201 ccttcagtca gttgggcaaa gccagtgtga tgatgctgac cagcaaagag acaattttgc
     4261 cgctgtttgg cgcgttgatt ctggtcggtt gcagtattta ctcgcgccgc tatttcaccc
     4321 gttttcttga acgttcggcg gcgaaagtcg gcaaagtgac tcaggatcac ttctggctga
     4381 cgttacgcac tcttttctgg tcgattctcg tcgcgtcacc gttgccggtg ctgtggatga
     4441 cgctgggtta cggcttgcgc gaggcgtggc cttatccgct ggcggtcgcg attggcgatg
     4501 gtgtaacggc caccgtgccg ctgctgtggg tagtgatgat ttgcgccacc tttgcccgcc
     4561 cgaacggctt gtttatcgct cattttggct ggccgcgcga acgtgtttcc cgtgggatgc
     4621 gctactacct gatgagcatc gggcttattg tgccgctgat tatggcgctg atgatgttcg
     4681 ataacctcga cgaccgtgaa ttctccggtt cgctgggacg gctttgcttt atcctcattt
     4741 gcggtgcgct ggcggtggtc accctcagcc tgaaaaaggc cgggatccca ctgtatctca
     4801 acaaagaggg cagcggcgac aacattacca accatatgct gtggaacatg atgattggcg
     4861 cgccactggt tgccattctg gcgtcggcgg tgggttatct ggcaacggca caggcgctgt
     4921 tagcgaggct tgaaacctcg gttgccatct ggttcctgct actggtggtt tatcacgtta
     4981 tccgccgctg gatgctgatc caacgccgca ggctggcgtt tgaccgggcg aagcatcgcc
     5041 gggcagagat gttagcgcaa cgtgcgcgtg gcgaagagga agcacatcat cacagtagcc
     5101 cggaaggggc aattgaagtc gatgaaagcg aagtcgatct cgatgccatc agtgcgcaat
     5161 ccttgcggct ggtgcgctca attttgatgt tgatcgccct gctttcggtc attgtgctgt
     5221 ggtcagaaat ccattccgct ttcggcttcc tcgaaaatat ttcgctgtgg gatgtcacct
     5281 ccacggtaca gggcgtagaa agtctggagc caattaccct cggtgcggtg ctgattgcca
     5341 ttctggtgtt tatcatcacc acgcagctgg tgcgcaacct gcccgcgctg ctggaactgg
     5401 cgattttgca gcacctggat ttaacgccgg gtacgggtta cgccatcacc accatcacca
     5461 aatatctgct gatgctgatt ggcgggctgg tcggcttctc aatgattggt attgagtggt
     5521 cgaaattgca gtggctggtt gccgcgctcg gtgttggtct cggttttggt ttacaggaaa
     5581 ttttcgccaa ctttatctct ggcctgatca tcctgttcga aaaaccgatt cgcattggcg
     5641 atacggtaac gatccgcgat cttactggta gcgtgacgaa aattaatacc cgcgcaacca
     5701 ccatcagcga ctgggaccgt aaagagataa tcgtgccgaa caaggcgttt attaccgagc
     5761 agtttatcaa ctggtcgctc tctgactcgg tcacgcgcgt ggtgttgacg attccggccc
     5821 ctgccgatgc caacagtgaa gaagtgacgg aaatcctgct caccgcagcg cgtcgctgct
     5881 cgctggtgat cgacaacccg gcaccggaag tcttcctggt ggatctgcaa caagggattc
     5941 agattttcga gctgcgtatt tacgccgctg agatgggtca ccgtatgccg ctacgccatg
     6001 agatccacca gctgattctg gcaggcttcc atgcccacgg tatcgatatg ccattcccgc
     6061 ccttccagat gcgtctggaa agcctgaatg gtaaacaaac ggggagaaca ttaacttctg
     6121 cgggcaaagg tcgtcaggcg ggaagtttgt aatgcatgaa gctgccaaat gatgtcaacg
     6181 tggctcattt agcagcttaa catcatcttc agttatcacc ggccctggag agtatcatcc
     6241 atacgcatta cttcctgaca actcatcgcc ggagagacga tgagttgcag gatttcatat
     6301 gatcagtcga gagctaactc aggtttattc gccatgcgac tgcaatagtt ctggtaaatc
     6361 gccatcgcta acagcgagaa gaagacaggg ccgccaatca tccacaatgt gctgtcccag
     6421 tctccagctt caaccacagg ttgaatgatg gtgaagacgt tcgcaaatgt caccaccagt
     6481 acgacaacca ctgttgcaat cattgccgac atatgcgttt tgaaaatcac aaatggtctg
     6541 tcgagatcct gacgtgcttt aaaaaatggg aaagccagcg cgaggaacag gtaaggaagc
     6601 gtcatagaca cgttcgccat cagcgtcagc ttgttaaaga acgccgatgc ggtaccgcca
     6661 ccaaacgaaa ccagcaggat gaagacagta accaacccgc actgcatcca catggcgata
     6721 gacggcatcc ccatcgcatt caggcgcgtc atcggttccg gccacaacgc tttcggcgtc
     6781 ccctggatga tggctttcag cggtgaatag caaagcgtaa agaacgcacc ggtataagcg
     6841 aggaacatcg aaagtccggt aatacgcgca aaccatacgc ccagcgacaa tgacgcttca
     6901 ggtgacaaat gcagtgcgtt acccagcgtc accccgaggc tcttcatcag cacataggta
     6961 atattgccaa ggttaacgga accattactt aatacctgct gccagtttgt gctgacgccc
     7021 cataaaaata ttgccagcga ataaccgatt gaaataacaa tagcggcaaa aacaatacct
     7081 ttggcaaagt tcttttctgg attttccgtt ttatcgacca gaccaccaac cgcttcaatt
     7141 ccgccatagg caaaaatagc aaataccaca aacgatagca ttgccagacc ggactgataa
     7201 cccgggttcg gtgatgcaag gaaattaata tcctgcgcga aatgcccacc atttaataac
     7261 aaaatagtaa tgcttactaa cagcaatact aaattcagac acattactgc aataccgccc
     7321 accgcagtaa tgcgggcaat tttattaatc ccctttgaag caacgacggt gaccagaatc
     7381 atccatgcta ctgccagcag accaaccacc tgcgtaggct ccagtccggc aatacgccag
     7441 tgcagggtca tgtcgctacc atagaggaat gttgagaacg gtacccaaac tttcgcggag
     7501 gtactcacca tccagatgat ataagaggaa aaccacataa acgtaccaat gaaggcaaaa
     7561 cgtggtccga cactattatt catccaggaa tagataccac cttcttcttt gcgataagca
     7621 gctcccattt cagccatcat taaggcgaat ggaataaaga ataacaatgc agaaaatata
     7681 taaaagggaa tcgcactata acccattaag taataagccg atgggctatt ggcaaatcca
     7741 aatacggaag taaagatcat caatatgagt cctatcagac tcatcttttt tatcgtgtga
     7801 ggcatgaaac catccttcat ttcagcgcgt gtataacacg tgcccactga actgcactgc
     7861 gcaacattga gcaggcagcg taaaaaccaa tacagaattg atacctggat gatatcaaat
     7921 atccagtctt aaattgtggc ctttaacgga aaaatgaaaa catatgctac agaatggcgc
     7981 ttatcacgcc attcttcgct gttaattcag taatttttca gaattatgcc cggtcaacgc
     8041 taaaggcgat gacttcagcc agtgtctccg cgcccagcgc caacatcacc agacgatcaa
     8101 cacctaatgc cacgccggaa cagtcaggca taccgacttt caaggcttca atcagattct
     8161 ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc
     8221 gttgttgctg ctcacgggca tccgtcaatt catggaaacc attcgccagc tcaatacctt
     8281 tataataaac ctcaaagcgt tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg
     8341 atgcctggct ggctggaaag tggtacacaa aggtcggttt ttctttgcca atatttggct
     8401 ctacgccaaa ggtaaacagc aattgtagca gcgtgtcgcg gtcttcttcg gtatcagcaa
     8461 cattgctcaa atccagtttc gctgcgactt cccgcagttg cgttttgtcg gcagagagcg
     8521 ggtcaatttc cagataacgc aagaaagctt gttgataaga aaggctttct gctgccgggc
     8581 agtccagcac ctgttgtaag agatcgtcca cctcgttcat caaccggtac atatcatagt
     8641 gcggtcgata ccactccagc atagtgaact cagggttgtg ataacgcccc atctcttcat
     8701 tacggaagct gcggcacagc tggaataccg gcccacaacc ggcaaccagc aggcgtttca
     8761 tatggtattc cgggctggtc attaaccaga gattcatccc ctgcgaatgc ccggggccaa
     8821 cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac cgtcgcctgg ctcatacaag
     8881 gcgtctccac ctccagcact ccacgatcgg caaagaaacg acggatctcc gccataatcg
     8941 ccgcgcgttt taataagtta ggaatggatg cgctcggctg ccaggatgcc gtttcgctca
     9001 tagttaaatc tccagttttt gacaagggca cgaagtctac tcgcaacgcg acggcgagac
     9061 aaattttacg caggaatcaa acagcggtgg gcagtgacta aaaaaagcac gatctgatgg
     9121 tttagtaatt aaattaatca tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat
     9181 cgatctcgtc aaatttcaga cttatccatc agactatact gttgtaccta taaaggagca
     9241 gtggaatagc gttcgcagac cgtaactttc aggcacttac cctgaagtac ggggctgtgg
     9301 gataaaaaca atctggagga atgtcgtgca aacctttcaa gccgatcttg ccattgtagg
     9361 cgccggtggc gcgggattac gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa
     9421 aatcgcacta atctcaaaag tatacccgat gcgtagccat accgttgctg cagaaggggg
     9481 ctccgccgct gtcgcgcagg atcatgacag cttcgaatat cactttcacg atacagtagc
     9541 gggtggcgac tggttgtgtg agcaggatgt cgtggattat ttcgtccacc actgcccaac
     9601 cgaaatgacc caactggaac tgtgggggtg cccatggagc cgtcgcccgg atggtagcgt
     9661 caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc tggttcgccg ccgataagac
     9721 cggcttccat atgctgcaca cgctgttcca gacctctctg caattcccgc agatccagcg
     9781 ttttgacgaa catttcgtgc tggatattct ggttgatgat ggtcatgttc gcggcgtggt
     9841 agcaatgaac atgatggaag gcacgctggt gcagatccgt gctaacgcgg tcgttatggc
     9901 tactggcggt gcgggtcgcg tttatcgtta caacaccaac ggcggcatcg ttaccggtga
     9961 cggtatgggt atggcgctaa gccacggcgt tccgctgcgt gacatggaat tcgttcagta
    10021 tcacccaacc ggtctgccag gttccggtat cctgatgacc gaaggctgcc gcggtgaagg
    10081 cggtattctg gtcaacaaaa atggctaccg ttatctgcaa gattacggca tgggcccgga
    10141 aactccgctg ggcgagccga aaaacaaata tatggaactg ggtccacgcg acaaagtttc
    10201 tcaggccttc tggcacgaat ggcgtaaagg caacaccatc tccacgccgc gtggcgatgt
    10261 ggtttatctc gacctgcgtc acctcggcga gaaaaaactg catgaacgtc tgccgttcat
    10321 ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt aaagaaccga ttccggtacg
    10381 tccgaccgca cactacacca tgggcggtat cgaaaccgat cagaactgtg aaacccgcat
    10441 taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct
    10501 gggctccaac tccctggcgg aactggtggt cttcggccgt ctggccggtg aacaagcgac
    10561 agagcgtgca gcaactgccg gtaatggcaa cgaagcggca attgaagcgc aggcagctgg
    10621 cgttgaacaa cgtctgaaag atctggttaa ccaggatggc ggcgaaaact gggcgaagat
    10681 ccgcgacgaa atgggtctgg ctatggaaga aggttgcggt atctaccgta cgccggaact
    10741 gatgcagaaa accatcgaca agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat
    10801 caccgacacc tccagcgtgt tcaacaccga cctgctctac accattgaac tgggccacgg
    10861 tctgaacgtt gctgaatgta tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc
    10921 acaccagcgt ctggacgaag gttgcaccga gcgtgacgac gtcaacttcc tcaaacacac
    10981 cctcgccttc cgcgatgctg atggcacgac tcgcctggag tacagcgacg tgaagattac
    11041 tacgctgccg ccagctaaac gtgtttacgg tggcgaagcg gatgcagccg ataaggcgga
    11101 agcagccaat aagaaggaga aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg
    11161 tgcgctataa cccggaagtc gataccgcac cgcatagcgc attctatgaa gtgccttatg
    11221 acgcaactac ctcattactg gatgcgctgg gctacatcaa agacaacctg gcaccggacc
    11281 tgagttaccg ctggtcctgc cgtatggcga tttgtggttc ctgcggcatg atggttaaca
    11341 acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta caccgacggt atgaaggttg
    11401 aagcgttagc taacttcccg attgaacgcg atctggtggt cgatatgacc cacttcatcg
    11461 aaagtctgga agcgatcaaa ccgtacatca tcggcaactc ccgcaccgcg gatcagggta
    11521 ctaacatcca gaccccggcg cagatggcaa agtatcacca gttctccggt tgcatcaact
    11581 gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa cccagagttc atcggtccgg
    11641 ctgccattac gctggcgcat cgttataacg aagatagccg cgaccacggt aagaaggagc
    11701 gtatggcgca gttgaacagc cagaacggcg tatggagctg tactttcgtg ggctattgct
    11761 ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa
    11821 gttcgaaaga ctttcttatc gcgaccctga aaccacgcta aggagtgcaa catgacgact
    11881 aaacgtaaac cgtatgtacg gccaatgacg tccacctggt ggaaaaaatt gccgttttat
    11941 cgcttttaca tgctgcgcga aggcacggcg gttccggctg tgtggttcag cattgaactg
    12001 attttcgggc tgtttgccct gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt
    12061 ttacaaaacc cggttatcgt gatcattaac ctgatcactc tggcggcagc tctgctgcac
    12121 accaaaacct ggtttgaact ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa
    12181 atgggaccag agccaattat caaaagtctc tgggcggtaa ctgtggttgc caccatcgta
    12241 atcctgtttg ttgccctgta ctggtaagga gcctgagatg attaatccaa atccaaagcg
    12301 ttctgacgaa ccggtattct ggggcctctt cggggccggt ggtatgtgga gcgcaattat
    12361 tgcgccggtg atgatcctgc tggtgggtat tctgctgcca ctggggctgt ttccgggtga
    12421 tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct
    12481 gttcctgatg atcgttctgc cgctgtggtg tggtttacac cgtatgcacc acgcgatgca
    12541 cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc tacggtctgg ctgctatcct
    12601 gacagttgtc acgctgattg gtatcgttac aatctaacgt atcgccaatg taaatccggc
    12661 ccgcctatgg cgggccgttt tgtatggaaa ccagacctta tgttcaaaac gacgctctgc
    12721 gccttattaa ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt
    12781 gtgcatcgca caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg
    12841 gtaatttatc agggtaaacc ttattacttt acctggggct atgcggacat cgccaaaaag
    12901 cagcccgtca cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc
    12961 gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cacaacaaaa
    13021 tactggcctg aacttaccgc taaacagtgg aatgggatca cactattaca tctcgcaacc
    13081 tacactgctg gcggcctgcc attgcaggtg ccggatgagg tgaaatcctc aagcgacttg
    13141 ctgcgcttct atcaaaactg gcagcctaca tgggcgccag gaacacaacg tctgtatgcc
    13201 aactccagta tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag
    13261 caggcgatgc aaacacgtgt cttccagcca ctcaaactca accatacgtg gattaatgta
    13321 cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt
    13381 tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt cgaccattga agatatggcc
    13441 cgctgggtac gaagcaatat gaatccccgt gatatcaacg acaaaacact tcagcaaggg
    13501 atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg cctgggctgg
    13561 gaaatgctgg actggccggt aaatcctgac agcatcatta acggcagtgg caataaaatt
    13621 gcactggcag cacaccctgt aaaagcgatt acgcccccaa ctcctgcagt acgcgcatca
    13681 tgggtacata aaacaggggc aaccggcgga tttggtagct atgtcgcgtt tattccagaa
    13741 aaagagctgg gtatagtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc
    13801 gccgcctggc agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac
    13861 cttttctgcg cttttccttg ctgtcatcta cacttagaaa aaaaccagta aggaaacatt
    13921 tatgcgcctg ctccctctcg ttgccgcagc gacagctgca tttctggtcg ttgcctgcag
    13981 ttctcctacg ccgccgcgtg gcgtaacagt agtgaataat ttcgacgcaa aacgttatct
    14041 cggtacctgg tatgagattg cccgttttga tcaccgtttt gaacgtggac tggaaaaagt
    14101 caccgcaaca tacagtctgc gtgatgacgg cggcctgaat gtcatcaata aaggctataa
    14161 ccctgacaga gaaatgtggc agcagagtga agggaaagcg tactttaccg gcgcaccaac
    14221 acgcgctgcg ctgaaagtgt cattctttgg tcctttctat ggcggttata acgtcattgc
    14281 acttgatcgg gaataccgcc atgcgctggt ttgcggcccg gaccgcgact acctgtggat
    14341 actctcccgc acgccaacca tttctgacga agtgaaacag gagatgctgg cagtcgcgac
    14401 ccgggaaggg tttgatgtca gtaagtttat ttgggtacag cagcccggta gttagtgagt
    14461 gctgagtttc agaccaataa tccccaatac gattaacgcc agactcgcca ggcgcatcgg
    14521 gttagcggac tcaccgagca gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc
    14581 cgtccacacg gcataagccg tccctaccgg taacgatttc atcgcccagg caagtagcgc
    14641 catactgact atcatcgccg tcacggtaat aacactcggc gtcaaacggc taaagccgtg
    14701 ggtatatttc aggccaacgg cccataccac ttccagcaga ccagcaataa ctaagataat
    14761 ccaggacata tcaggctccg gagaattggg gccgtccccg gtgaaaagag gcgtttgcag
    14821 gtcgtcctgc aaagctgatg gttgaacgga tattttgcca ggtggcaggg gaatttcaac
    14881 tattaccttt gtgactatcg caaaaaaaca aacggcacga cataactgtc gtaccgttat
    14941 tgcttattgc tgcgctttcg ttgctgcacc agaaatcgcg ttaccgccat cagaaatgtc
    15001 ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact gttgaaagca ccagaacaga
    15061 aaagatcgct gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa tgttatttat
    15121 gtttgcctat agcaaattaa taatagacaa catcgccaaa aatgatggaa atctgatgat
    15181 ttttcggaag agaagaaaaa ttagctggca gcgcgggaga tggagttgcc gagatgtttg
    15241 atgtcttcgc cgaaaccgcg agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa
    15301 acaagaacga taaggcgttt catcatctct gacctgaata agtgatggtg cagcctgcag
    15361 gccgcaccac aaccgcatta cttcacgcga gagacgtatt caccagagcg ggtatccact
    15421 ttgatgactt cgccgatttg tacgaacagc ggaactttaa ccacagcgcc agtagacagg
    15481 gtagccggtt tgccgccagt acctgcggta tcacctttca ggcccggatc ggtatcaacg
    15541 atttccagtt caacgaagtt cggcggagta acggagatcg gctgaccatt ccacagagtt
    15601 acgatacact ctgcctgatc cagcagccat ttagcgttgt cgccaattgc ttttgcatca
    15661 gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac
    15721 aggtaagtca ggttcatatc gacaacatca gcgccttcag cggaatcagt agatttgaag
    15781 gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg
    15841 cctttacccg gttttacgaa ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg
    15901 attttaagac cagcacgaaa atcgttgcta tagtacgttg ccataaggcc ctctgaaatt
    15961 tgttaattgg tagctaagcc acaaaatggc gcatattgta accctaaata ccccatccag
    16021 agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct
    16081 tttgaatata gacgcggacg aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc
    16141 cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa ggcaatccgg acgatcctct
    16201 tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc gcgtccggat tctccaccga
    16261 cccgctggaa gaacagcaca gcgtagtgcc tggtttgttg cataaatacc acaaccgggc
    16321 gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc gtcgccactt
    16381 cccctatgcc gaaaatcagg gcaacaagcg taactggcag actgctcttg agtatgttgc
    16441 tgcgcatccg gaactggacg agatgatttt ctccggcggc gatccgctga tggcgaaaga
    16501 tcacgagctg gactggttgc tcacacaact ggaagccatc ccgcatatca aacgtctgcg
    16561 gattcacagc cgtctgccga ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg
    16621 ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt
    16681 agatgaaacg ttccgtcagg cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa
    16741 ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa acgctggcaa acctgagtaa
    16801 tgcgttgttc gatgccggcg taatgccgta ttacctgcat gtgctcgata aagtacaggg
    16861 cgcggcgcat tttatggtga gtgatgacga agcacggcag attatgcgtg agttgctgac
    16921 actggtgtcg ggatatctgg tgccgaaact ggcgcgagaa attggcggcg aacccagcaa
    16981 aacgccgctg gatctccagc tacgccagca gtaactgatt aaacagggaa gttaaacatt
    17041 agcctgatgc gctacgctta tcaggcctac attgtgcatt gaaattgaat ggatttgcaa
    17101 tgaactgtag gccggataag gcgtttacgc cgcatccggc aagagcaaca aacgcaagta
    17161 cagcaacctg tcattaaact gggaaattta atctttgccc gttgcataac ctttaattat
    17221 tcatatacat tgctgttgtt actctttata tagccaacac caataattaa cctttctttt
    17281 ct
//
LOCUS       DAZFRN010000538        16891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.guided.105, whole genome shotgun sequence.
ACCESSION   DAZFRN010000538 DAZFRN010000000
VERSION     DAZFRN010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.guided.105"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            complement(80..1792)
                     /locus_tag="ACHKUB_005480"
                     /pseudo
     CDS             complement(80..1792)
                     /locus_tag="ACHKUB_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            1764..2549
                     /gene="lafU"
                     /locus_tag="ACHKUB_005481"
     CDS             1764..2549
                     /gene="lafU"
                     /locus_tag="ACHKUB_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO6658311.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            2620..3675
                     /gene="dinB"
                     /locus_tag="ACHKUB_005482"
     CDS             2620..3675
                     /gene="dinB"
                     /locus_tag="ACHKUB_005482"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO6658312.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELECRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3672..4124
                     /locus_tag="ACHKUB_005483"
     CDS             3672..4124
                     /locus_tag="ACHKUB_005483"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6658313.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            4314..5453
                     /locus_tag="ACHKUB_005484"
     CDS             4314..5453
                     /locus_tag="ACHKUB_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6658314.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFVELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            5450..6064
                     /gene="prfH"
                     /locus_tag="ACHKUB_005485"
     CDS             5450..6064
                     /gene="prfH"
                     /locus_tag="ACHKUB_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6658315.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(6121..7578)
                     /gene="pepD"
                     /locus_tag="ACHKUB_005486"
     CDS             complement(6121..7578)
                     /gene="pepD"
                     /locus_tag="ACHKUB_005486"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6658316.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            7839..8297
                     /gene="gpt"
                     /locus_tag="ACHKUB_005487"
     CDS             7839..8297
                     /gene="gpt"
                     /locus_tag="ACHKUB_005487"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6658317.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            8389..9633
                     /gene="frsA"
                     /locus_tag="ACHKUB_005488"
     CDS             8389..9633
                     /gene="frsA"
                     /locus_tag="ACHKUB_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6658318.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            9691..10092
                     /gene="crl"
                     /locus_tag="ACHKUB_005489"
     CDS             9691..10092
                     /gene="crl"
                     /locus_tag="ACHKUB_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6658319.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(10131..11186)
                     /gene="phoE"
                     /locus_tag="ACHKUB_005490"
     CDS             complement(10131..11186)
                     /gene="phoE"
                     /locus_tag="ACHKUB_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO6658320.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINYQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            11474..12577
                     /gene="proB"
                     /locus_tag="ACHKUB_005491"
     CDS             11474..12577
                     /gene="proB"
                     /locus_tag="ACHKUB_005491"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO6658321.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            12589..13842
                     /gene="proA"
                     /locus_tag="ACHKUB_005492"
     CDS             12589..13842
                     /gene="proA"
                     /locus_tag="ACHKUB_005492"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO6658322.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNSSTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            13957..14032
                     /locus_tag="ACHKUB_005493"
     tRNA            13957..14032
                     /locus_tag="ACHKUB_005493"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13990..13992,aa:Thr,seq:cgt)
     gene            complement(14159..14497)
                     /locus_tag="ACHKUB_005494"
                     /pseudo
     CDS             complement(14159..14497)
                     /locus_tag="ACHKUB_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(14548..15504)
                     /gene="paoD"
                     /locus_tag="ACHKUB_005495"
     CDS             complement(14548..15504)
                     /gene="paoD"
                     /locus_tag="ACHKUB_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO6658323.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(15515..>16891)
                     /gene="paoC"
                     /locus_tag="ACHKUB_005496"
     CDS             complement(15515..>16891)
                     /gene="paoC"
                     /locus_tag="ACHKUB_005496"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO6658324.1"
                     /translation="IPNNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPET
                     AVQQSELLYAGANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDP
                     VEFRILNDTQVDPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHG
                     VAAGFRNNLLEKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVA
                     VHLGDSSFPVSAGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITN
                     GTRSATLHEATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRML
                     AVCAAGRILNPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHAD
                     IPKQEVIFLDDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKL
                     LDKLPDVV"
BASE COUNT         3961 a         4411 c         4532 g         3987 t
ORIGIN      
        1 taggccggat aaggcgttca cgccgcatcc ggcggttgtg taccggtgcc agatgcgacg
       61 cagacgcgtc ttatcatgcc tatatcagcg cccccattat cttcaactcc agctcatccg
      121 gcacttcgtt atacgacagc acatgcagcc ccggcgcaaa caaccttgcg tagcgcgcca
      181 gcaaggggcg cagctgcggc ggcaccagca gcaccggatc tttccccgcc gctttcatct
      241 gctccttcac ctgaggcatc gtgctctgga actggttgag catattcgga tccactggca
      301 cgctatcaag catcactttg ccggcctgtt gcgcctgatt caccacgttg gtcagcagat
      361 tttccagctc attattcagc gtatacaccg tcagctcctg cttgcgaacg aacggatggg
      421 taatgctgcg ccgcagcgcc aggcgcacat cggccgccag cagaatatga tctttagtca
      481 ccgcactact ggcgaccaac acggtggcga tggtgacgat atcgcgcagg gaaacgcctt
      541 cagtcagtag cgcccggtac actttcagca actggctgta attgagcgcc gcgctcaaat
      601 cttccgccag tcgcggtgcc atcgacgaca aacggttatg cagctgagta atatcgtcat
      661 agttaaacaa atcaggaata tagctgcgca caatcttatt cacatgggtg gcaattacgc
      721 tggcgctgtc gatcacctga taccccatat tcagcgcctt cgctttctgc gcgggctgaa
      781 tccaggtgac cggcatcccg tacgccgggt cgttccccag cacgccgtct atctcgccgt
      841 aggtttcgct ggagggcagg gccatcagtt tatctgccgg aatatccgct tcatcagcct
      901 taatgccgtt gatgaaaatg gcgtactggc tgggcttgag gcggaagttt tcccgaatac
      961 ggatctccgg cagcagcacg ccgttgccgt cggaaatcac ctgacgtacg ccgcgaatac
     1021 tctgggtgag cgggttgccc tgcgctttat ccaccagcgc caccagtttg tagccgaggc
     1081 ttaaactgat aggttcgatc agcggaatag tttcccaact gacctgttgc tcgctggttt
     1141 ctgtcatcgt acgggttagc gtttcgaggc ttttctcttc cgcctccgcc gcttgcgggc
     1201 gcttgctcat ccgccagccg gtaaaaccaa gcagggcgct gaacatcagg aacgggaaat
     1261 gcggcattcc cggcaccacc gccagcacga acataattcc agtggcggtg tagagaaccg
     1321 acgggcttgc cagcaactga ttacgcacgt catgggcgat atcaccgtta tcgctgacgc
     1381 gggtgacgat aatcgccgcc gcggtggaga gcagcaggga agggatttgc gccaccagcc
     1441 catcgccgat agtcatcagc acatattgct ggaaggcggc gtcggcgctc aggttgtatt
     1501 tgaagatgcc gatacagacg ccgccgatca ggttgattgc cagaatcatc atcccggcga
     1561 tggcgtcccc gcgcacaaat tttgatgccc cgtccattgc gccgtagaaa tcggcctcgc
     1621 tggcgacatc tttacgccgg gtttgcgcct gcgtctggtt gatcaacccg gcgttaagat
     1681 cggcgtcaat cgccatctgt ttgccgggca tcgcgtccag ggtaaagcgg gcagaaacct
     1741 cggaaatacg ctcggccccc ttggtgacga cgataaagtt gattgtcaac agcgtcagta
     1801 aatccgagcg tgaaagcatt atcgccgcgc tgcacgggca gtcgattttc aatggtggcg
     1861 ggctgtcgcc gttaaataaa atcagcccgt ctcatccgcc aaaacctgcc accgtggcgg
     1921 tgccggaaga gacggagaaa aaagcgcgcg acgtcaacga gaaaacggcg ctgctgaaga
     1981 agaaatccgc caccgagctt ggcgagctgg caaccagtat caataccatc gcccgtgatg
     2041 cgcatatgga agcgaatctg gagatggaga ttgttcccca gggattacgc gtgctgatta
     2101 aagacgacca gaaccgcaat atgtttgaac gcggcagcgc gaagattatg ccgttcttta
     2161 aaacgctgct ggtggagctt gcgccggtgt tcgactctct cgataacaaa attattatta
     2221 ccgggcatac cgatgcgatg gcctacaaaa acaatatcta caacaactgg aacctttcgg
     2281 gcgatcgcgc gctttcggct cgtcgggtgc tggaagaggc cggaatgccg gaagataaag
     2341 tgatgcaggt aagcgcaatg gcggaccaga tgttgctgga tgccaaaaat ccgcaaagcg
     2401 cgggcaaccg gcgcattgag attatggtgc tgaccaaaag tgcgtccgat acgctgtatc
     2461 aatactttgg tcagcatggg gataaagtgg tgcagccgct ggtgcaaaag ctggataagc
     2521 agcaggtgct ttcgcagcga gcgcgttaaa tgctgaatct ttacgcattt ctcaacccct
     2581 gaaattactg tatactttac cagtgttgag aggtgagcaa tgcgtaaaat cattcatgtg
     2641 gatatggact gctttttcgc agcggtggag atgcgcgata atcccgccct gcgcgatatc
     2701 cctattgcta ttggcggcag ccgcgaacga cggggggtga tcagcaccgc caattatccc
     2761 gcgcgtaaat ttggcgtacg tagcgctatg ccgacaggga tggcgctcaa attatgcccg
     2821 catctcacct tgcttccagg gcgctttgac gcctacaaag aagcctcaaa tcatatccgc
     2881 gaaattttct cgcgctatac ctcgcgcatt gaaccgttat cactggatga agcctatctc
     2941 gatgtcaccg atagcgtcca ttgccacggt tctgcgaccc tcatcgccca ggaaatccgc
     3001 cagacgatct tcaacgagct gcaactgacg gcgtctgcgg gcgttgcgcc ggtcaagttt
     3061 ctcgccaaaa ttgcttccga catgaataaa cccaacggtc agtttgtgat tacgccggca
     3121 gaagttccgg catttctgca aaccttaccg ctggcaaaaa ttcccggcgt cggcaaagtc
     3181 tcggcggcaa aactggaagc gatggggctg cggacctgcg gtgatgtgca aaagtgtgat
     3241 ctggtgattc tgcttaaacg ctttggcaaa tttggccgca ttttgtggga gcgtagtcag
     3301 gggattgacg agcgcgacgt taacagtgaa cggttgcgaa aatccgtcgg cgtggaacgc
     3361 acgatggcgg aagatattca tcactggtct gaatgtgaag cgattattga gcggctgtat
     3421 cctgaacttg aatgccgtct ggcaaaggta aaacctgatt tactgattgc tcgccagggg
     3481 gtgaaattaa agttcgacga ttttcagcaa actactcagg agcacgtctg gccgcggctg
     3541 aataaagctg atctaatcgc caccgcgcgt aaaacctggg atgaacgtcg cggcgggcgc
     3601 ggtgtgcgac tggtggggct gcatgtgacg ttgcttgacc ctcaaatgga aagacaactg
     3661 gtgctgggat tatgaataac atacaaataa gaaactatca gcctggtgat tttcagcaac
     3721 tatgcgctat tttccttcga gctgttacga tgacggcgag tcagcactat tctccacagc
     3781 aaattgccgc ctgggcgcag attgacgaat ctcgctggaa ggagaaactc gcgaaatcac
     3841 aagtgcgggt tgcagttatt aatgcacaac cggttggttt tattacccgc attggacatt
     3901 atatcgatat gttatttgtt gaaccggaat acacccgacg cggagttgcc agcgctttgt
     3961 taaaaccatt gattaagtct gaatccgaac ttacggtgga cgccagcata accgcaaaac
     4021 ccttttttga acgttatggt tttcagacag ttaagcagca gcgcgttgaa tgccggggaa
     4081 cgtggtttac taatttttat atgcgatata aaccgcaaca ttaaatccag cttgcaatga
     4141 aaataacgcc cgtctagtat gtgcgcgttt tcttattcca caaactgcaa ggaggtaaat
     4201 catgacaaat cctttatcaa tgactctttg cagacctttc caggattaat tcttttttct
     4261 tgccctggat tcgtctgcca tttcctgatt tttattcata aatggaatgg tacatgggca
     4321 aatatattcg tcccttatcc gatgcggtat ttaccatcgc atctgatgac ctgtggatcg
     4381 agagtttagc gatccaacaa ttacacacca cggcaaattt acccaacatg cagcgcgttg
     4441 tggggatgcc agatttacat cccggacgcg gctacccgat tggcgcagcg ttcttctctg
     4501 ttggtcgttt ttacccggca ctggtcggca atgatatcgg ctgcggcatg gcgctatggc
     4561 aaacagatat tctcgctcgc aaatacaacg ccgataagtt tgaaaagcga ttatcagatc
     4621 tggatgacgt tgctgaagaa agctggctgg aggaaaacct gccgtcagca tttgcacagc
     4681 atccgtggcg caactcgctt ggctccatcg gtggcggtaa ccacttcgta gaactgcaac
     4741 agattgatca aattatcgac gcggaactgt ttgcactggc tggtctggat gcgcagcatc
     4801 tgcaactgct ggttcatagc ggctcgaggg gattggggca gtctatttta cagcggcata
     4861 ttgcctcgtt ttcgcatcat ggtttgcctg aaggcagtga cgacgcgcta cggtatattg
     4921 cggaacatga cgatgcgctg gcgtttgcgc gtattaatcg ccagctgatc gctttgcgca
     4981 taatgcaaca agttaaggcc accggaagtc cggttctgga tgtggcgcat aactttgtta
     5041 gcgcgtgcca aatcggcgat caacagggct ggttgcatcg taaaggcgcc acgccggatg
     5101 acaacggtct ggtgattatt cccggttcac gcggcgatta ctcctggctg gttaaacctg
     5161 ttgcgaacga aaaaacgctc cactcgctgg cgcatggggc ggggcgtaaa tgggggcgca
     5221 ccgagtgtaa agggcgtctg gcagcgaaat acacggcgac gcaactctcg cggactgaac
     5281 ttggcagtcg ggtaatttgt cgcgataaac aactcatctt tgaagaagcg ccacaagcct
     5341 ataaatcggc tgaaagcgtg gtgcaatgtc tggtgctggc tgggttaatt attcctgtcg
     5401 cgcgactgcg cccggtgctg acgctcaaaa acagtggagg gaaaaaagga tgatcttgct
     5461 acaactctcc tctgctcagg ggccggaaga atgttgtctc gcagtgagaa aagcactgga
     5521 caggctgatt aaagaagcta cccgacagga cgtcgcggta acggtgctgg aaacagaaac
     5581 gggtcgctac tctgacacac tgcgttcggc gctgatttct ctggatggcg ataacgcatg
     5641 ggctctaagc gaaagctggt gcggcactat tcagtggatt tgtccgagtc cgtatcggcc
     5701 tcatcatggg cgcaaaaact ggtttctggg cattgggcgt tttaccgctg atgagcagga
     5761 acaatcggat gcaatccgtt atgagacgct gcgttcgtcg gggccgggcg gtcaacatgt
     5821 caataaaacc gactcggcgg tacgcgccac gcatctggca tccggtatta gcgtgaaggt
     5881 tcagtcagag cgcagtcagc atgctaacaa acggctggcg cgattactga ttgcctggaa
     5941 gctggaacaa cagcaacagg aaaatagcgc ggcgctgaaa tcgcagcggc gaatgttcca
     6001 tcaccagatt gaacgtggca acccgcgacg aacgtttaca gggatggcct ttatcgaagg
     6061 ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca aatcaaataa
     6121 ttacttcgcc ggaatttctt tcagcagttc agtcagcagt gtccagtaat ggccaacgct
     6181 ttcgatatga acttgctcat ccggagagtg tggaccggtg atggttggcc cgatagacac
     6241 catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg cgtggataat
     6301 ctggatgttc ggcgttttgt tgaacagacg ctgataggtt tcacgtacca gatgcattac
     6361 cggagagtta gcgtccggct gccagccagg atatgcgcct tttgcttctg ttttcgcacc
     6421 tgccagttta cccagtgaat ccagcatgct cactacgtag tctttaccgc tgtcgatcag
     6481 agaacggatc aggcagtgaa tttctacgtt attgtcggtc atggtcacca caccgacgtt
     6541 cagggaggtt tcgaccacac cttttgccac gtcggagttg cggatcacac cgttcggggt
     6601 ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag ctttatcgtt
     6661 cgcaacagag tccagcagca atgccagatt tttctctttc tctgccagtt cgtttttcag
     6721 gatctcctga taggtatcca ccagagattt cagggcgtcg actttatcag ctgcgacagc
     6781 aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt tgaaatcaat
     6841 aagacgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccaaca gtttgttggc
     6901 attacccagg ccaacgtgga tttcaccgcc ggagtgaccc cctttcagac cttttaaggt
     6961 taacttgaag gtttcaaaac cagctggaac cgcttcacga tctaaatgca ggttggaggt
     7021 gaagtcgata ccccccgcac aacccatgta gatttcacct tcttcttcgg agtcggtgtt
     7081 aatcagaata tcagcctgca accagttgct ctgtaagccg aacgcaccgt ccataccggc
     7141 ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag
     7201 aaccgccagc gcagacgcca taccaatgcc gttatctgca cccagcgtgg tgccgcgcgc
     7261 tttaacccat tcgccatcaa tataaggctg gataggatct ttcgtgaagt catgcacggt
     7321 gtcgttattt ttctgcggca ccatatcgag gtgggcctgt aagacgaccg gtttacgatt
     7381 ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac
     7441 atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata
     7501 ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa
     7561 ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg
     7621 tcacagggtt agcagaaaat tttgtcaaca caagacaggc ttgcgagata tgtttgagaa
     7681 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     7741 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     7801 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     7861 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     7921 aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg ttactggcgc
     7981 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     8041 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     8101 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     8161 actttgtcac catctttgca aaaccggctg gtcgtccgct ggttgatgat tatgttgttg
     8221 atatcccgca agatacctgg atcgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     8281 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     8341 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     8401 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     8461 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg ctcttgatgg taaaaccatc
     8521 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggtat tgacccacgt
     8581 gaaatcctag acgtccaggc acgtattgtg atgagcgatg ctgaacgtac cgacgatgat
     8641 ttatacgata ctgtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccacccag
     8701 gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag tggtcgacac
     8761 tggctgcatg cggctacgtt gtacaacatt gccgcctatc ctcatctgaa aggagatgac
     8821 ctggccgagc aagcgcaggc tttatcaaac cgcgcctatg aagaggccgc ccagcgtctg
     8881 ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt
     8941 ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat
     9001 gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg
     9061 atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct cacccaggac
     9121 tccagcctgt tgcatcagca cgtcttaaag gcgctgccta acgtaccgtg ggtggatcac
     9181 actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatac
     9241 cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc cggtagttca taccctgttg
     9301 agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct ggcgagtcgt
     9361 ttggggatgc atgatgcttc cgacgaagcg ttgcgcgtgg agttgaatcg ctattcatta
     9421 aaagtgcaag gattgctggg acgtcgctgc ccaacgccaa tgttatcagg ctactggaag
     9481 aacgatccgt tcagcccgga agaggactca cgcttaatca cctcatcatc tgctgacggt
     9541 aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg tcttcaggaa
     9601 atcaccgact ggatcgaaaa acgtttgtgt taaaaatttg ctaaatttta ccaatttggt
     9661 aaaacagttg catcacaaca ggagattgca atgacgttac cgagtggaca cccgaagagc
     9721 agattgatca aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa gtgcgaagat
     9781 aatcgattct ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc accggaagtg
     9841 cgtgagttct ggggctggtg gatggagctg gaagcgcagg aatcccgttt tacctacagc
     9901 taccagtttg gtctgttcga taaagcaggc gactggaaga gtgttccggt aaaagatact
     9961 gaagtggttg aacgactgga gcacaccctg cgtgagtttc acgagaagct gcgtgaactg
    10021 ctgacgacgc tgaatctgaa gctggaaccg gcggatgatt ttcgtgacga gccggtgaag
    10081 ttaacggcgt gagtgaaatg tgccggatgc atcacatccg gcaatatcta ttaaaactga
    10141 taggtcatgc caaccgcgac aatatcatca ttattaatat tcaatttgtt atcgctatcc
    10201 agttggttga ttttataatc aacaaacgct gacatatttt tgttgaaata atacgtagca
    10261 ccgacgtcga tataattgac cagatcttca tcaccgatgc cctcaatatc tttccctttc
    10321 gataagacat aacccagcga tggacgcaga ccaaagtcga attgatattg tgcgaccgct
    10381 tcaaagttct gagttttatt ggcaaagccg ccagatattg gcgtcatttt gcgtgtttca
    10441 gaataaaaag ttgccagata aatattattg gcatcgtatt tcagacctgt tgcccatgct
    10501 tctgcacgct tgcctgtgcc acggctttgc aggttctgct cgttggtgcg atcggagttg
    10561 gtataggccc cactaatggc gaaatcgctg ccgccaaagt catatgtcaa tgacgtgccg
    10621 aagccatcgc cgttttgctt tttaacatcg cggttttcgt ttttcccttg atattgcagg
    10681 gttaagttca gaccatcgat aacgccgaag aagtcggtgt tccgatacgt cgccagaccg
    10741 ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg agtcgccacc aaattccggg
    10801 aacatatcgg tccaggcttc cacgtcatac aacgcgccca ggttacgacc ataatcgaaa
    10861 gaacccaaat ctttatattt caacccggca aaagcgagac gcgttttttg ctgtgcagta
    10921 tcactctccg ctttatttcc ggcaaactct gcttcccaac ggccataacc agtcagttga
    10981 tagttaattt gtgtttcacc tttaaaacca aaacggatat aactctggtc gccatcttta
    11041 ctgtcgttat cactcatata atgcatggct ttaactttgc catagacatc cagtttatta
    11101 ccgtctttgt tatatatttc tgcggcctgt acggatgcag atgccacaat gcccatcacc
    11161 actaatgcca gagtgctctt tttcattttc attcctgatt ttaattaacg cgcgaatatt
    11221 cagcgggaga gtcccgttga aaacaggaaa gtttttaacc tgagattgtt aaagatatat
    11281 tacagattaa taatattctt aaaatgtggt aatttattaa atctgtaata aaagcgtaaa
    11341 caactgccgc taggcttgct gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg
    11401 ttggcaaccg acgacagtcc tgctaaaacg ttcgtttgat atcatttttc ctaaaattga
    11461 atggcagaga atcatgagtg acagccagac gctggtggta aaactcggca ccagtgtgct
    11521 aacaggcgga tcgcgccgtc tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc
    11581 gcagttacat gccgccgggc atcggattgt tattgtgacg tcgggcgcga tcgccgccgg
    11641 acgtgagcac ctgggttacc cggaactgcc agcgactatc gcctcgaaac aactgctggc
    11701 agcggtaggg cagagtcgac tgattcaact gtgggaacag ctgttttcga tttatggcat
    11761 tcacgtcggg caaatgctgc tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa
    11821 cgcccgcgac accctgcgtg cgttgctcga taacaatatc gttccggtaa tcaatgagaa
    11881 cgatgctgtc gctacggcag agattaaagt cggcgataac gataaccttt ctgcgctggc
    11941 ggcgattctg gcgggtgccg ataaactgtt gctgctgacc gatcaaaaag gtttgtatac
    12001 cgctgacccg cgcagcaatc cgcaggcaga actgattaaa gatgtttacg gcattgatga
    12061 cgcactgcgc gcgattgccg gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac
    12121 caaattgcag gccgctgacg tggcttgccg tgcgggtatc gacaccatta ttgccgcggg
    12181 cagcaagccg ggcgttattg gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca
    12241 tgcccaggcg actccgcttg aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg
    12301 tgaaatcacg gtagatgaag gggcaactgc cgccattctt gaacgcggca gctccctgtt
    12361 gccgaaaggc attaaaagcg tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg
    12421 caacctcgaa ggtcgcgata tcgcccacgg cgtcagtcgt tacaacagcg atgcattacg
    12481 ccgtattgcc gggcaccact cgcaagaaat tgatgcaata ctgggatatg aatacggccc
    12541 ggttgccgtt caccgtgatg acatgattac ccgttaagga gcaggctgat gctggaacaa
    12601 atgggcattg ccgcgaagca agcctcgtat aaattagcgc aactctccag ccgcgaaaaa
    12661 aatcgcgtgc tggaaaaaat cgccgatgaa ctggaagcac aaagcgaaat catcctcaac
    12721 gctaacgccc aggatgttgc tgacgcgcgt gccaatggcc ttggcgaagc gatgcttgac
    12781 cgtctggcac tgacgcccgc acggctgaaa ggcattgccg atgatgtgcg ccaggtgtgt
    12841 aacctcgccg atccggtggg gcaggtaatc gatggcagcg tactggacag cggcctgcgt
    12901 cttgagcgtc gtcgcgtgcc gctgggggtg attggcgtga tttatgaagc gcgcccgaac
    12961 gtgacggttg atgtcgcttc gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt
    13021 ggcaaagaaa cctgtcgcac taacgcggca acggtggcgg tgattcagga cgccctgaaa
    13081 tcctgtggct taccggcggg tgccgtgcag gcgattgata atcctgaccg tgcgctggtc
    13141 agtgaaatgc tgcgtatgga taaatacatc gacatgctga tcccgcgcgg cggggctggt
    13201 ttgcataaac tgtgccgcga acagtcgaca atcccggtga tcacaggtgg tataggcgta
    13261 tgccatattt acgttgatga aagcgcagag atcgctgaag cattaaaagt gatcgtcaac
    13321 gcgaaaactc agcgaccgag cacatgtaat acggtagaaa cgttgctggt gaataaaaac
    13381 atcgccgata gcttcctgcc cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta
    13441 cacgcagatg cagctgcgct ggcgcagttg cagacaggcc ctgcgaaggt ggtggcggtt
    13501 aaagccgaag agtatgacga tgaatttctg tcattagatt tgaacgttaa aatcgtcagc
    13561 gatcttgacg atgccatcgc ccatattcgt gaacacggca cacaacactc cgatgcgatc
    13621 ctgacccgcg atatgcgcaa cgcccagcgt tttgttaacg aagtggattc gtccgctgtt
    13681 tacgttaact cctctacgcg ttttaccgac ggcggccagt ttggtctggg tgcggaagtg
    13741 gcggtaagca cacaaaaact ccacgcgcgt ggcccaatgg ggctggaagc actgaccact
    13801 tacaagtgga tcggcattgg tgattacacc attcgtgcgt aaataaaacc gggtgatgca
    13861 aaagtagcca tttgattcac aaggccattg acgcatcgcc cggttagttt taaccttgtc
    13921 caccgtgatt cacgttcgtg aacatgtcct ttcagggccg atatagctca gttggtagag
    13981 cagcgcattc gtaatgcgaa ggtcgtaggt tcgactccta ttatcggcac cattaaaatc
    14041 aaagagttac cccatattta aatacaccac gtttcctcct gtgccgcatt tgtgccattg
    14101 taaccttggc aattcatcaa aatactgttc tgacatcagg cagtgcaggt gcagctattt
    14161 aaaccaattg ctgccgccat tctttgacgt agtcaatcag ggcgcggagc tttggtgcaa
    14221 tattgcgacg ctgtgggaaa tacagataga agcccggaaa ttgtggaaga aagtcatcaa
    14281 gcagcgatac aagcttaccg ctttcaatat atggcctgaa agtttcctga gtggcaattg
    14341 ttattcctcc gccggcaaga gccagcctca acatcagacg cagatcatta gtcgtaatct
    14401 gcggttcaat cgcaaggtcg aaagttctcc cgttttcttc aaatggccag cgataaggcg
    14461 caacctccgg ggactgacgc cagccgatac acttatgggt atttcccccg gaggcgagaa
    14521 agcgctctcc acgcccggcc gcaaggatca ggacgacggg ggcaggcatg aatcctcctc
    14581 ctgatggaga cgtacagagg cgacttctgc cagcacggag agtgccagag tatgcgcatc
    14641 ccgggctttg gggaatatcc cgacgggtgc ccggatttgc gttgtttcct ccctggacca
    14701 tcccagctcg tggagctttt gcagacgtaa cgtgtgggtt cgatagctgc ccaatgcgcc
    14761 gagataaaag ggttttgctt ctcgcgcggc ctgcaacact ggcagctccc ggttgagatc
    14821 atggcacagc aaaatgaccg ccgtatcggt atcgatctga gcgctggctg aggccggaaa
    14881 aagatcgaag atatggctgt catagcctgt ggctgctgca agactcgcag ttgcctgcgc
    14941 ctcaagagaa cgtccgtaaa tcatcagcct gacgcatggc ctgaacccca cctcaaagcc
    15001 attgagattc cagcccgtcc gggtttgcgt gggcaggcac accagcgatt gtgcttgcgg
    15061 atcgtagcgc agccccgccg gttttctctg ttccagacgg ttcagcacgg cgagcagagg
    15121 ctgtgccgag cgtagtttat ggagcgtcag cgtgatccca ccgccgcagg gcagaacgat
    15181 gtcaaaccac ggcgaacctt cgccatagcg aatttcgcga tcgcggcctg agcccatcat
    15241 ctccagcgct tcaaaagcgg cagcggcctc cacgcagccg ccagagacaa aaccgcagta
    15301 acgaccatct tcgcgaacca ccatctgcgc cccgagcggg cgcgctgcgc cgccgcgtat
    15361 ctccaccagc gtcaccagca ccgctccttt accggacatt agcgcctcta cggcgaagcg
    15421 cagaatggtc cggtggtcat cggtgagaaa cgcctgctct ggcttatgcc agtgctcgtc
    15481 tttgtcaaaa agcgggtatg acattgtttt ctccttaaac cacatccggc agcttatcga
    15541 gcagcttatc cagagtgatg ggataatccc gtacccgaat accggtggcg ttatacaccg
    15601 cgttggcgat agccgcgctc acgccgcaca ggcccagctc accgacacct ttggccttca
    15661 tcggggagga tatggggtcg gtatcatcca ggaaaatcac ctcctgtttt gggatatccg
    15721 catggaccgg cacctcatac cccgccatat cgtgattaac gaagtagccc aaacggtcat
    15781 ccaccgccag ctcctccatc agtgccgcgc ccatgcccat agtcattgcg ccaatgacct
    15841 ggctgcgcgc agttttcgga ttcaggatgc gtcctgcagc acacacagcg agcatacgcc
    15901 ggacccgaac ttctcccgtc gcgctatgca cgccgacctc cacaaaatgc ccggcaaagg
    15961 tcgactgctg gtactctttg ctcagtgttc cgaattcaat gctctcttcc gctgtcagtc
    16021 tgccgcctgc ggtggcttca tgtagcgtgg cgcttcgggt accgttggta atcttgccgt
    16081 cggcaaactg cgactgctca ggatcaaacc cgactgccga ggcaatcatt tcgcgaagct
    16141 tcatacaggc ggcgtaaacg ccggaggtgg aggtattcgc gccccattgt ccaccagaac
    16201 ccgcagaaac cgggaaactg gaatcgccga ggtgaaccgc aacctgctcc agcggtacgc
    16261 caagcatttc cgctgccgtc tgggccagaa tggtgtagct gccggtgcca atgtcggtca
    16321 tgtccgtttc tacggtaacg gtgccgtttt gttcgaggtg aacccgagca cccgattttt
    16381 ccagcagatt attgcgaaag cccgccgcaa caccgtggcc gactagccac tccccgtcgc
    16441 gcacctgtcc gggtgtggcg ttgcgctgct tccagccaaa tttatccgct ccggtgcgca
    16501 agcactcgat aagctgacgg cgagagaagc agcgcgtcgg gtcggcgggg tcaacctgag
    16561 tgtcattcag gatgcgaaac tcgacgggat cgatgcccgc tttttccgcc agttcgtcga
    16621 tcgcgatttc gagcgccatc agaccggggg cttcgccggg cgcacgcatg gcgttccctt
    16681 ccggcaaatc aagcgtggcg agccgcaggc cggtatgacg attcgccccg gcgtagagta
    16741 attcgctttg ctgtaccgcc gtttccggcg tgccgccggg caggtttcca gaccagcttt
    16801 catgtgagat agcggtgatt ttcccgctct ggtcggcacc gatacgcaag tgctgaaggg
    16861 tggcggggcg gtgcgtggtg ttattgggaa t
//
LOCUS       DAZFRN010000539         4450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.guided.269, whole genome shotgun sequence.
ACCESSION   DAZFRN010000539 DAZFRN010000000
VERSION     DAZFRN010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.guided.269"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            18..620
                     /locus_tag="ACHKUB_005497"
     CDS             18..620
                     /locus_tag="ACHKUB_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFO6658325.1"
                     /translation="MEHDGKQWFTAATLASALEYSRGDKITQIYNRNSDEFTPCMSIN
                     LKMRFNGINNSLREKDVRIFSLRGAHLVAMFATTPVAKEFRRWVLDILDREAEKSAIP
                     LQPHIQPQFTAEDIILLCYVQLWMEKAQQLSKQLYPVMRELNSDYYGKLFDLAYETRH
                     ITNRTRDTLLREAAKLDPANYMVHRARSMLAQLRARQFEF"
     gene            675..1085
                     /locus_tag="ACHKUB_005498"
     CDS             675..1085
                     /locus_tag="ACHKUB_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO6658326.1"
                     /translation="MNQTIFLRSKQQQQFAINAILATTLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMVADISRQVQWCGRWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTSWAVEQGVRFTDRRYE"
     gene            1082..1273
                     /locus_tag="ACHKUB_005499"
     CDS             1082..1273
                     /locus_tag="ACHKUB_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6658327.1"
                     /translation="MRRQRRSITDIVCENCNYLPTKRSRNKPRPIPNESDVKTFNYTS
                     HLWDVRWLRRRARFNSHSG"
     gene            1297..1587
                     /locus_tag="ACHKUB_005500"
     CDS             1297..1587
                     /locus_tag="ACHKUB_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6658328.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGRGMK
                     PDDLLAAWACSDCHNEIDRRTRILDNNDARLYHLEGVIRTQAILLKEGKIKS"
     gene            1584..1946
                     /locus_tag="ACHKUB_005501"
     CDS             1584..1946
                     /locus_tag="ACHKUB_005501"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6658329.1"
                     /translation="MNEYEFVLPWPPTVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LRLDIFTKSRLRITIIAEPPDSRRRDLDNILKGLLDSLIYAGFAEDDEQFDDIRVIRG
                     VKVPGGRVGIKITELENI"
     gene            1946..2140
                     /locus_tag="ACHKUB_005502"
     CDS             1946..2140
                     /locus_tag="ACHKUB_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO6658330.1"
                     /translation="MNAKIQTIPELLICTRGNQTEVARILNCNRATVRKYIDDKDAKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            2133..2567
                     /locus_tag="ACHKUB_005503"
     CDS             2133..2567
                     /locus_tag="ACHKUB_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6658331.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            3075..4022
                     /gene="stxA1c"
                     /locus_tag="ACHKUB_005504"
     CDS             3075..4022
                     /gene="stxA1c"
                     /locus_tag="ACHKUB_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1c subunit A"
                     /protein_id="HFO6658332.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSVNAILGSVALILNCHHHASRVARIVP
                     NEFPSMCPVDGRVRGITHNKILWDSSTLGAILIRRAISS"
     gene            4033..4302
                     /gene="stxB1c"
                     /locus_tag="ACHKUB_005505"
     CDS             4033..4302
                     /gene="stxB1c"
                     /locus_tag="ACHKUB_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1c subunit B"
                     /protein_id="HFO6658333.1"
                     /translation="MKKILLIAASLSFFSASVLAAPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT         1261 a          912 c         1096 g         1181 t
ORIGIN      
        1 cccacgttct tgaaacgatc gaacacgacg gcaaacagtg gttcactgcc gcgactttgg
       61 cttcagcact ggagtattcg cgcggcgata aaatcaccca gatatacaac cgcaattcgg
      121 atgagtttac tccttgtatg tcaatcaacc tcaaaatgag gttcaacggg atcaacaata
      181 gcttacgaga gaaagatgtg cgtattttct ctctacgcgg tgcccacctg gtagcgatgt
      241 tcgcaacaac tccagtagcc aaagagttcc gtcgctgggt attggatatt ctggaccgtg
      301 aagctgaaaa aagcgctata ccgctacaac ctcatattca gccgcaattc acagcagaag
      361 acatcatcct actctgctac gttcaactct ggatggaaaa agcacaacaa ctcagtaagc
      421 agctataccc cgtcatgaga gaactgaact ctgattacta cgggaaactg tttgatctgg
      481 cctacgagac tcgtcatata accaaccgga ctcgcgacac attgctccgc gaagcagcaa
      541 aacttgatcc cgccaactat atggtgcata gggccagaag catgctggcg caactacggg
      601 caagacaatt tgaattctga aactaaagga gcttcggctc cttttttgtt gggaaaatcc
      661 aatgagaggg aataatgaac cagactatct tcctccgaag taagcagcag caacaattcg
      721 ccattaacgc catccttgca acaactctcg ataaagacaa acccgttacg atccgcatca
      781 ccgattacaa gcggaatctc gatcaaaatg ccaaatttca cgcgatggtc gctgatatct
      841 cccgacaggt tcagtggtgc ggcagatggc taaaaccaga acagtggaaa gttttgttaa
      901 tcagcggtca tgccgtggcg acaaaacagg aagctgatgt tttgccaggt cttgaaggtg
      961 aatacgtcaa tatccgcgaa agcagtgcgc agatgagcgt gaagcgtatg gcaagcctga
     1021 ttgagtacac aacttcctgg gctgtggagc agggtgtcag atttaccgac aggaggtatg
     1081 aatgagacga cagcgacgaa gcattaccga tatagtctgt gaaaactgca attaccttcc
     1141 aacgaaacgc tcccgaaata aacccaggcc aatccccaac gaatctgatg taaaaacctt
     1201 caattatacg tctcacctgt gggatgtccg gtggctcaga cgtcgtgcaa gatttaatag
     1261 tcactccgga tagttcaatg tacgaggaat aagatgatgg caaacctacg caaagaagcg
     1321 cgtggcagag aatgtcaggt acggatttac ggcgtatgca atggcaatcc tgaaactaca
     1381 gttttggcgc attaccgaat ggctggaatt tgcggaaggg gaatgaagcc agatgacctg
     1441 ttggctgcat gggcctgtag tgactgccat aatgaaatcg atcgccgtac tcgcattctc
     1501 gacaacaacg acgccagact ttaccacctg gaaggcgtga tcaggacgca ggcgatactg
     1561 ctgaaggagg ggaaaattaa gtcatgaatg aatatgagtt tgtgcttccc tggccgccga
     1621 cggtgaatac ctactggcga agacggggaa gccagtacta catcagcgat aaaggccaga
     1681 aataccgaaa agacgtacag caaatcatcc ggcaactcag attagacatt ttcactaaat
     1741 cacgacttcg catcacaatt attgctgaac caccagattc ccgtcgtcgc gacctcgata
     1801 acatcctgaa aggtttactc gactctctta tctacgccgg atttgcggaa gacgacgagc
     1861 aattcgatga cattcgcgta attcgcggcg tgaaagtgcc tggcggtaga gtggggataa
     1921 aaatcaccga actggagaac atttgatgaa tgctaaaatt caaacgatac ctgaattact
     1981 gatctgcacc aggggaaatc agacagaagt cgccagaata ctgaactgca atcgtgccac
     2041 agtcagaaaa tacattgatg ataaagatgc gaaaaagcac gccgtcgtca atggcgtcct
     2101 tatggttcat cgcggatggg gtaaagatac tgatgcgtga tatccggcag gttcttgagc
     2161 gctggggtgc atgggcggca aataactatg aggatgttac atggtcgccc attgctgccg
     2221 gatttaaggg actgatcccc gaaaaagtaa aatcacgtcc acagtgttgt gacgatgacg
     2281 cgatgattat atgcgggtgt atggctcgcc ttaacaggaa caacagtgat ctgcatgact
     2341 tgctggttga ttattacgtg ttgggggaga cgttcatggc gctggcacgg aaacatgggt
     2401 gctctgacac ctgtataggt aaacgccttc acaaagcgga ggggattgtt gaaggcatgc
     2461 tgatgatgct gggagtgagg cttgagatgg atcggtatgt tgagcgtgaa ttgccgggag
     2521 ggagaacctc tgtattttat cagcgaaaaa atagtttacg atcgtaaaaa tctgcatatc
     2581 atgataagag tggttacatt gctacgctgc ttaacccgcc gatgcgcggg tttttttgta
     2641 cccagaatcc tgtgagctat acggaaagta cacagaaagg aaggtgcgac cgtaattaat
     2701 aacaaaatct taaaaatcgc atatagcact attagttttc taaatattgt atattttaag
     2761 tattgcagga tgaccctgta acgaagtttg cgtaacagca ttttgctcta cgagtttgcc
     2821 agcctccccc agtggctggc tttttttatg tccgtaacat cctgtgtatc aataaatgtt
     2881 gttgtctacg tacgtcaagt agtcgcatga gatctgacca gatatgttaa ggttgcagct
     2941 ctctttgaat atgattatca ttttcattac gttattgtta cgtttatccg gtgcgccgta
     3001 aaacgccgtc cttcagggcg tggaggatgt caagaatata gttatcgtat ggcgctcaag
     3061 gagtattgtg taatatgaaa ataataattt ttagagtgct aacttttttc tttgttatct
     3121 tttctgttaa tgtggttgcg aaggaattta ccttagattt ctcgacagca aagacgtatg
     3181 tagattcgct gaatgtcatt cgctctgcaa taggtactcc attacagact atttcatcag
     3241 gaggtacgtc tttactgatg attgatagtg gcacagggga taatttgttt gcagttgatg
     3301 tcagagggat agatccagag gaagggcggt ttaataatct acggcttatt gttgaacgaa
     3361 ataatttata tgtgacagga tttgttaaca ggacaaataa tgttttttat cgctttgctg
     3421 atttttcaca tgttaccttt cctggtacaa ctgcggttac attgtctggt gacagtagct
     3481 ataccacgtt acagcgtgtt gcggggatca gtcgtacggg gatgcagata aatcgccatt
     3541 cgttgactac ttcttatctg gatttaatgt cgcatagcgg aacctcactg acgcagtctg
     3601 tggcaagagc gatgttacgg tttgttactg tgacagctga agctttacgt tttcggcaaa
     3661 ttcagagggg atttcgtaca acacttgatg atctcagtgg gcgttcttat gtaatgactg
     3721 ctgaagatgt tgatcttacg ttgaactggg gaaggttgag tagtgtcctg cctgactatc
     3781 atggacaaga ctctgttcgt gttggaagaa tttcttttgg aagtgttaat gcaattctgg
     3841 gtagcgtggc attaatactg aattgtcatc atcatgcatc gcgagttgcc agaattgtac
     3901 ctaatgagtt tccttctatg tgcccggtag atggaagagt gcgtgggatt acgcacaata
     3961 aaatattgtg ggactcatcc actctggggg caattttgat acgcagggct attagcagtt
     4021 gaggggggta aaatgaaaaa aatattatta atagctgcat cactttcatt tttttcagca
     4081 agtgtgctgg ctgcgccaga ttgtgtaact gggaaggtgg agtatacaaa atataatgat
     4141 gacgatacct ttacagttaa agtgggagat aaagaattat ttactaacag atggaatctt
     4201 cagtctcttc ttctcagtgc acaaattact gggatgacgg taaccattaa aactaatgcc
     4261 tgtcataatg gagggggatt cagcgaggtt attttccgtt gactcagaat agcccggtga
     4321 agagaacagg cggagattta tcaatgttaa atacatctca attcagtcag ttgattgttg
     4381 cctgtctgat aatagatgtg ctacaaattt ctgcatggtg aatccccctg agcggcgggg
     4441 catatcagcg
//
LOCUS       DAZFRN010000540         1522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306S61014
            SAMN43283753-rid23079033.guided.365, whole genome shotgun sequence.
ACCESSION   DAZFRN010000540 DAZFRN010000000
VERSION     DAZFRN010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283753
            Sequence Read Archive: SRR30325467
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351729.1
            Genome Coverage       :: 131x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:27:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 94
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 71 of 168
            Pseudo Genes (incomplete)         :: 106 of 168
            Pseudo Genes (internal stop)      :: 37 of 168
            Pseudo Genes (multiple problems)  :: 38 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283753-rid23079033.guided.365"
                     /serotype="-:H19"
                     /isolate="2306S61014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx1c; stx2b"
     gene            132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHKUB_005506"
     CDS             132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHKUB_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO6658334.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFMIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHKUB_005507"
     CDS             1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHKUB_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO6658335.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          416 a          310 c          367 g          429 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca tacttatttt ttctggctcg
       61 cttttgcggg ccttttttat atctgcgccg ggtctggtgc tgattacttc agccaaaagg
      121 aacacctgta tatgaagtgt atattattta aatgggtact gtgcctgtta ctgggttttt
      181 cttcggtatc ctattcccgg gagtttatga tagacttttc gacccaacaa agttatgtct
      241 cttcgttaaa tagtatacgg acagagatat cgacccctct tgaacatata tctcagggga
      301 ccacatcggt gtctgttatt aaccacaccc caccgggcag ttattttgct gtggatatac
      361 gagggcttga tgtctatcag gcgcgttttg accatcttcg tctgattatt gagcaaaata
      421 atttatatgt ggctgggttc gttaatacgg caacaaatac tttctaccgt ttttcagatt
      481 ttacacatat atcagtgccc ggtgtgacaa cggtttccat gacaacggac agcagttata
      541 ccactctgca acgtgtcgca gcgctggaac gttccggaat gcaaatcagt cgtcactcac
      601 tggtttcatc atatctggcg ttaatggagt tcagtggtaa tacaatgacc agagatgcat
      661 ccagagcagt tctgcgtttt gtcactgtca cagcagaagc cttacgcttc aggcagatac
      721 agagagaatt tcgtcaggca ctgtctgaaa ctgctcctgt gtatacgatg acaccggaag
      781 aagtggacct cacactgaac tgggggagaa tcagcaatgt gcttccggag tttcggggag
      841 aagggggtgt cagagtgggg cgaatatcct ttaataatat atcagcgata ctgggcacag
      901 tggcggttat actgaattgc catcatcagg gggcgcgttc cgttcgcgcc gtgaatgaag
      961 agatacaacc agaatgtcag ataactggcg acaggccagt tataaggata aacaatactt
     1021 tatgggaaag taataccgca gctgcttttc tgaatcgcag ggcccactct ttaaatacat
     1081 ccggagaata acaggagtta aatatgaaga agatatttgt agcggcttta tttgcttttg
     1141 tttctgttaa tgcaatggca gctgattgtg caaaaggtaa aattgagttc tctaagtata
     1201 atgagaatga tacattcaca gtaaaagtgg ccgggaaaga gtactggact aaccgctgga
     1261 atctgcaacc gctactgcaa agcgcacagt taacaggaat gacggtaaca atcaaatcaa
     1321 atacctgtgc gtcaggttca ggatttgctg aagtgcagtt taattaatat cagaagcatt
     1381 gctggtttcg tggtgtgcag caatgtagtt acagtgtaat caatgtcaca attcagtcag
     1441 ttgaaggttg tctgcccgac tgagaatttg ttaaaaaaaa tcctgcatgg tgaatccccc
     1501 tgagcggcgg ggcatatcag cg
//
